LOCUS       DASWBY010000001         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_86, whole genome shotgun sequence.
ACCESSION   DASWBY010000001 DASWBY010000000
VERSION     DASWBY010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="VF870_00005"
     CDS             <1..1162
                     /locus_tag="VF870_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9250590.1"
                     /translation="LFYPPDPTETNCYIGGTVATNASGAKTFKYGPTRDYVIGLQIVF
                     PEGEVLDLERGKQKAIGYDLSLKTHSGKEVNLQIPDYTFPAVKNASGYFVKPDMEAID
                     LFIGSEGTLGIITKIKLKLLPQPEDTISCVLFFNDETTALNFLEEARDTSYQNKKNNI
                     SDNINALALEFFDERALKFLSKDFSAIPVNAQAAIWFEQESTSETFDAILEAWNNLMQ
                     NHNVNEETAWFAFSDADKEKIKDFRHAISWKVNEYVARNNFRKLGTDVAVPDNFFRDF
                     YFTLQNWAKQSNIDFVAYGHFGNSHIHLNFLPKNDDEFNEGKKLYKQICEEAVRLGGT
                     ISAEHGIGKAKRDYLLMMYGETNIRKMAALKKSLDPNLVLGIGNIFDERFLL"
     gene            complement(1224..>2626)
                     /gene="cysS"
                     /locus_tag="VF870_00010"
     CDS             complement(1224..>2626)
                     /gene="cysS"
                     /locus_tag="VF870_00010"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX9250591.1"
                     /translation="LYNTLTKKLDEFIPIDPNEVKFYVCGPTVYDYFHIGNARSFVMA
                     DIVRKYLEYKGYKVKFVMNLTDVDDKIIKKANEEKRKPDEVTNDYINAFFEDLTKLKI
                     KKANVYPKATEHMDEIIGMIKSLVDKKFAYNIDGNVFYDVKHFDQYGKLSGKNIEDLE
                     SGARIEINEEKSNPLDFALWKKAKEGEPYWESPWGKGRPGWHIECSAMSCKHLGETFD
                     IHAGGNDLIFPHHENEIAQSEAATGKPFVKYWLHFGFLNINEEKMSKSLGNFFTARDV
                     LKRYSAEAIRMFFAQAYYRGPLNFTDDLLAGAEKGLEKITNLVDKVNDELKKNNSGTK
                     PEFDFKSFYHRFEEAMDDDLNTSQAVAVIFDFVKEVNRIIASSENVDVSFYHDVKTFL
                     TKTAENVLGIVDFNKTTAGDDKLVNDLIGLLITLRTEAKQNKNYQLSDTIRDRLKELG
                     VVLQDSKEGTIYKIGR"
BASE COUNT          834 a          474 c          426 g          892 t
ORIGIN      
        1 tcttttttat ccaccggatc caaccgaaac taattgttat atcggcggaa cagttgcaac
       61 aaatgcttcc ggtgcaaaaa cttttaaata cggaccaaca cgtgattacg ttattggttt
      121 acaaatcgtt tttccagaag gagaagtgct tgatcttgaa cgtggaaaac aaaaagcaat
      181 tggatatgat ctatcattaa aaactcattc aggcaaagaa gtcaatttgc aaattccaga
      241 ttatactttt cctgcagtta aaaatgcatc gggttatttt gttaagcctg atatggaagc
      301 aattgattta tttatcggat ctgaaggcac actgggaatt attactaaaa ttaaacttaa
      361 acttcttccg cagccagaag atacaatttc ctgcgttctc ttctttaatg atgaaaccac
      421 tgcattaaat tttcttgaag aagcaagaga tacttcttat caaaacaaaa agaataatat
      481 tagtgataat ataaacgcac ttgctcttga attttttgat gaacgagcat taaaattttt
      541 gtctaaagat ttttctgcaa ttccggttaa tgcacaagcg gcaatttggt ttgagcagga
      601 atcaacttcc gaaacatttg atgcaattct tgaagcatgg aataacttga tgcaaaatca
      661 taatgtaaat gaagaaactg catggtttgc ttttagtgat gcagataaag aaaagattaa
      721 agattttcgt cacgcaattt cctggaaggt taatgaatat gtggcgcgca ataattttag
      781 aaaacttggg actgatgtgg ctgtccctga taattttttc cgtgattttt attttactct
      841 gcaaaactgg gcaaagcaaa gcaatataga ttttgttgct tatggtcatt tcgggaattc
      901 tcacattcac ttaaactttt taccaaagaa tgatgatgaa tttaacgaag gtaaaaagct
      961 ctacaaacaa atttgtgaag aagcggtaag acttggagga acaatctccg ctgaacacgg
     1021 aattggaaaa gctaaacgcg attatctttt aatgatgtat ggtgagacta atattcgcaa
     1081 aatggcagca ttaaaaaaat ctcttgatcc aaatcttgta cttggaattg gaaatatatt
     1141 tgatgagagg tttttactgt agcgcaggaa tcctttcctg cgattggtgt taaaaaagca
     1201 cgagaggatg cgcgctctac aagttatctc ccaatcttat aaatcgtacc ttctttacta
     1261 tcctgcaaaa caacgccaag ctcttttagt ctatcacgaa ttgtatctga aagctggtag
     1321 tttttatttt gttttgcttc tgttctaagt gtaataagta atcctatcaa atcatttaca
     1381 agtttatcat caccggcagt agttttgtta aaatcaacaa tgccaagaac gttttctgct
     1441 gttttagtta aaaatgtttt tacatcatga taaaaactta catcaacatt ttctgaggaa
     1501 gcaattattc ggtttacttc ttttacaaaa tcaaaaataa ccgcaactgc ctgtgatgta
     1561 tttaaatcgt catccatcgc ttcttcaaat cgatgataga aagatttgaa atcaaattca
     1621 ggtttagtgc cggaattgtt tttcttcaac tcatcattaa ctttatcaac aagattggta
     1681 atcttttcca gacctttttc tgctccggct aaaagatcat cagtaaaatt taaaggtccg
     1741 cgataataag cttgggcaaa aaacattctt attgcttcgg cagaatatct ttttaacaca
     1801 tctcttgctg taaaaaagtt gccaagagat ttagacattt tttcttcgtt gatattaaga
     1861 aaaccaaaat gcagccagta ctttacaaaa ggttttcctg ttgcagcctc actctgcgca
     1921 atttcatttt cgtgatgagg aaaaattaaa tcattgccgc cggcgtgaat atcaaatgtt
     1981 tcgccaagat gtttgcagct cattgcagaa cactcaatat gccagccggg tcttcctttt
     2041 ccccagggac tttcccagta aggttcgcct tcttttgctt ttttccacaa agcaaaatcc
     2101 agtggattag atttctcttc attaatttct attcgtgcac cggattcaag atcttcaata
     2161 ttttttccgc tcaactttcc atattgatca aaatgtttta catcataaaa aacatttcca
     2221 tcaatgttgt atgcaaattt tttatccaca agacttttaa tcattccaat tatttcatcc
     2281 atatgctcgg ttgctttagg ataaacatta gcctttttga tctttaactt agtgaggtct
     2341 tcaaagaaag catttatgta atcgtttgta acttcatccg gttttcgttt ttcttcattt
     2401 gccttcttaa tgatcttatc atcaacatca gtaagattca taacaaactt tactttataa
     2461 cctttatact ccaaatactt tcgcacaata tccgccataa caaacgatcg tgcattacca
     2521 atatgaaagt aatcgtacac agttggtccg catacataaa atttcacttc atttggatca
     2581 attggaataa attcatctaa tttttttgta agagtgttgt agagtt
//
LOCUS       DASWBY010000002         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_451492, whole genome shotgun sequence.
ACCESSION   DASWBY010000002 DASWBY010000000
VERSION     DASWBY010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451492"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VF870_00015"
     CDS             complement(<1..1176)
                     /locus_tag="VF870_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEX9250592.1"
                     /translation="MIKYLVVFTIMLSINSFAQVRVNADAIEFPQILTSLTDTSSFYI
                     TNESEQPAQIELNNLKSVYSMSDTSLIIPANDSSIVWIKYLPDQNVIDNDLIFITSDD
                     STIGNIISINGSAKYGDTYDLTTFNLYDNQLKNALTQLVTGHTSLGYNLARDKMFMEI
                     DNQKVNGQGAADNTLECVYTGRLAVGYTSRTVAQNPPYNFNTEHTWPQSNFGEAEPMK
                     SDLYHLYPTDITANGIRANYPFDEVVSNITWQVGGSKLGNNSLGQIVFEPRDVHKGDV
                     SRSMFYFITRYPVNYGGFFTQIQESVFREWNKLDPVGVVESNRNNAIASLQLKHNPFI
                     DHPEFVDRIYSFATSNVHPTFAELNVLPLKVEFDSTLINTSSSSQIYIANSGSAQLNI
                     "
     gene            complement(1254..1640)
                     /locus_tag="VF870_00020"
     CDS             complement(1254..1640)
                     /locus_tag="VF870_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9250593.1"
                     /translation="MSRKNISSGAKWEDIVGYSRAVRIGSIVEVSGTAPVDGDKVIGI
                     GDAYAQTKFCLQKIEKALIEAGAKLKDVIRTRMFVTDITLWEEYGKAHGEFFKDIKPV
                     TSMIEVKSLIDPQMLIEIEASAVIED"
BASE COUNT          536 a          310 c          261 g          583 t
ORIGIN      
        1 aatatttaat tgtgcactgc cgctgtttgc aatgtaaatc tgacttgatg atgaagtatt
       61 tattaaagtt gaatcaaact caactttaag cggaagcaca tttaactctg caaaagtggg
      121 gtgaacatta ctcgtggcaa aactataaat cctatcaaca aattccggat gatcaataaa
      181 aggattatgt ttcaactgca aacttgcaat tgcattattt ctatttgatt caactactcc
      241 taccgggtca agtttattcc actctctgaa aacactctcc tgaatttgag taaaaaatcc
      301 tccgtagtta acaggataac gagtaataaa ataaaacata gagcggctta catcaccttt
      361 atgaacatct cttggttcaa aaacaatttg ccctaaacta ttattaccaa gtttacttcc
      421 gccaacctgc caggtaatgt ttgaaacaac ttcatcaaat ggataatttg ctctgatacc
      481 atttgcagta atatcagttg gatacaagtg ataaagatct gatttcattg gttctgcttc
      541 accaaaattt gattgcggcc aggtatgttc agtattaaaa ttatatggtg gattttgtgc
      601 aacagttcga ctggtgtaac caacagcaag tcttccggta tatacacact cgagtgtgtt
      661 gtccgcagca ccttgtccgt ttaccttttg attatcaatt tccataaaca ttttatcacg
      721 agccaggtta tagcctagcg aagtatgtcc cgtcactaat tgtgttaaag cattttttaa
      781 ttgattatcg taaagattga aggttgtcaa gtcgtaggtg tctccgtatt tagcagagcc
      841 gttaatacta ataatgtttc ctattgtact gtcatcacta gtaataaata ttaaatcatt
      901 atcaattaca ttttgatcag gtaaatattt tatccaaaca atactgctat catttgccgg
      961 gataattaaa gaagtatcgg acatagaata aacgcttttc aaattattta attcaatttg
     1021 tgcaggctgt tcagattcat ttgttatata aaatgaagag gtatctgtta aacttgtaag
     1081 tatttgtgga aattcaatcg catcggcatt aactctaacc tgagcaaatg agttaattga
     1141 aagcataatg gtgaaaacaa ctaaatattt aatcatatat atagcgaata attatttaag
     1201 taaaattttg aggcataaat ttacaaccga attctgtaat aacaagactt atactaatct
     1261 tctattacag cactagcttc tatttcgata agcatttgcg gatcgatcaa tgatttaact
     1321 tcaatcatag aagttacagg ttttatgtct ttaaagaatt ctccgtgggc tttcccatat
     1381 tcttcccaga gtgtaatatc ggttacaaac attctggttc taataacatc ttttaatttt
     1441 gcacctgctt caattaatgc tttttcaatt ttctgtaagc agaattttgt ttgtgcataa
     1501 gcatcgccaa tcccaatcac tttatcacca tctacaggtg ctgtacctga tacttcaact
     1561 atgctgccaa ttcgtactgc acgtgagtaa ccgacaatat cttcccattt tgcaccggat
     1621 gaaatatttt ttcgactcat atttttcctt ccaaatatga tttacgtttt ctctcaggaa
     1681 acttttcaat
//
LOCUS       DASWBY010000003         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_150543, whole genome shotgun sequence.
ACCESSION   DASWBY010000003 DASWBY010000000
VERSION     DASWBY010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150543"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..755
                     /locus_tag="VF870_00025"
     CDS             132..755
                     /locus_tag="VF870_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250594.1"
                     /translation="MKKLFVKIFSVDLAIIFFFFAFMFYSGCSCKPCSEQEESKIPLS
                     ILKIADQFIASKTGEDFFKKYITVDFSQSKHIAPNYLMVYRFFMPEKPFVDELIRFNV
                     DSIGNVLTQYEVVGIPDCSGDPMHCDFVVDEKIAKQIAVENGLPKGIKDWKVDFIWEA
                     KYNKYIWRILSTSKETNLQDHNIAEGELIIIDPSNAMVIEKESWRVN"
     gene            759..>1897
                     /locus_tag="VF870_00030"
     CDS             759..>1897
                     /locus_tag="VF870_00030"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9250595.1"
                     /translation="MIVKEEKIKRKERKRERMLEVAAELFSKKNYHEVMMDDVARLTD
                     VAKGTVYNYFSSKEELYFTIMSSKLENLNTSLKNKIASEISVIDSLHTYVIHLYMFMM
                     KYKNFFLMYQKEYMNAQNEFCDELRAMSDELKSILSDIIYKGKRENQFRDVDESFSVK
                     LVLGSIFGAVQRGVENKYTGDKLIEEREKLFDFILHGLYSGFNNSKVRPLKNKTIVLT
                     RTVEQSKESAAIFSELGADVIVFPTLEIVPPGSWEKFDDLILSQEKIDFIVFTSAHAV
                     TMFIKRTNELKIQIDFERIKVVAVGNKTKSVCEENNIKVDILPKKFSGEGIVEELSKH
                     DLKDKLIFIPRSAIGREDLPKGLEELGAKITAVPVYNVSLPSKESV"
BASE COUNT          682 a          254 c          374 g          587 t
ORIGIN      
        1 ataacttatc gtaaaaaatt acgcggcagc aaaaatgatc ccgaaaaatt gggcaaaact
       61 cttgccaagg atttattaaa agccggtgcg aaagaaattc taaaagaagt ttatcaaact
      121 gcgagagcta aatgaaaaag ctgtttgtaa aaattttttc agttgattta gcaatcattt
      181 tctttttttt tgcatttatg ttttacagtg gctgttcatg caaaccatgt tccgagcaag
      241 aagaatccaa gattccactt agtattctta aaatagcaga tcagtttatc gcttctaaaa
      301 ccggtgaaga tttctttaaa aagtatatca ctgtagattt ttcacaatca aagcatatcg
      361 caccaaatta tttaatggtt taccgatttt ttatgcccga aaaaccattc gttgatgagt
      421 taataagatt taatgttgat agcatcggta atgtgttaac ccaatacgaa gtggttggaa
      481 ttccggattg tagcggagat cctatgcact gtgattttgt tgttgatgag aagatcgcaa
      541 aacaaattgc tgtagaaaat ggattaccaa agggcattaa agattggaaa gtggatttta
      601 tttgggaagc taagtacaat aaatacatat ggagaatatt gagcacgagc aaagaaacaa
      661 atttacaaga ccacaatata gctgaaggtg aattgataat aattgatcct tcaaatgcaa
      721 tggttattga aaaggaatcc tggagagtta attaggttat gatagttaaa gaagaaaaaa
      781 taaagcgcaa ggaaagaaaa cgcgagcgaa tgcttgaagt tgccgccgaa cttttttcta
      841 aaaagaatta tcacgaagta atgatggatg atgtagcccg cttaactgat gttgcaaagg
      901 gaacagtata caattatttc agttcgaaag aagagctata ttttactata atgagctcta
      961 aacttgagaa tcttaacaca tcactaaaaa ataaaattgc gtcagaaata agtgttattg
     1021 attcactgca cacgtatgtg atacatctat atatgtttat gatgaaatat aagaatttct
     1081 ttttgatgta tcagaaagaa tatatgaatg cacagaatga attttgtgat gaacttcgtg
     1141 caatgagtga tgagttgaaa tctattttaa gtgatattat ttataaaggc aaaagagaaa
     1201 atcaatttcg tgatgttgat gaaagtttta gtgtaaaact ggtgcttgga agtatatttg
     1261 gcgcggttca acgcggagtt gaaaataaat acaccggaga taaattaatt gaagaacgcg
     1321 aaaaactttt tgatttcatt cttcatggtt tgtattcagg ttttaataac agcaaggtaa
     1381 gaccgctaaa aaataaaact attgtactta caagaacggt tgagcaatct aaagaatctg
     1441 ctgcaatatt tagcgagctc ggtgccgatg taatagtttt tccgacatta gagattgttc
     1501 caccaggcag ttgggaaaag tttgatgatt taattttgtc tcaggagaaa attgatttta
     1561 tagtcttcac ttctgcacac gcggttacaa tgtttattaa gaggactaat gaattaaaaa
     1621 ttcagatcga ttttgagaga ataaaagttg ttgcggttgg aaataaaaca aaatcggtat
     1681 gtgaggaaaa taatatcaaa gttgatatct tacctaaaaa attttctggc gaggggattg
     1741 ttgaagaatt atctaaacac gatttaaaag ataagcttat ctttattccg cgttctgcaa
     1801 tcggaagaga agatttgcca aaaggtcttg aagaacttgg agcaaaaatt acagctgttc
     1861 ctgtttataa tgtttcactt ccgtcaaaag aatctgt
//
LOCUS       DASWBY010000004         7251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_75344, whole genome shotgun sequence.
ACCESSION   DASWBY010000004 DASWBY010000000
VERSION     DASWBY010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75344"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VF870_00035"
     CDS             complement(<1..822)
                     /locus_tag="VF870_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HEX9250596.1"
                     /translation="MIFYAGINLGFGSGYKSQVNEAMLQAACYNLISDLRNLNYNNEY
                     DAGIIKPDSEKKKNEALSITDNSFSPDSPFAAIDDSIFSPEDSSGLSNLLDSTKVTGK
                     DSLKTKVKVNQDSLRLVQMALDSTARLQYFRYQREDVPYTTLKQKKKSKFFVEPSPTF
                     KSRIISIDSTGKYVEIKEIVAGKPNKIVLRIPIDEYIKTRLEVEERKKWEALGYSYEL
                     KDSKLGLGDFMKSLTDFEIPLPSVGVLSIFGTPKISLKIGGSVQIHGAWRSETTEG"
     gene            complement(1085..1585)
                     /locus_tag="VF870_00040"
     CDS             complement(1085..1585)
                     /locus_tag="VF870_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9250597.1"
                     /translation="MNQKAKYYIEKLQLIKHPEGGYFREVYRSGEIITIEAPKKNLKR
                     NFSTSIYFLLEGSQVSKFHRLKSDELWHFYDGGSVKLYVIGEKGKLTEIILGKKTEDG
                     EVFQTVIKKNNWFAAEVINKRSFSLIGCTVSPGFDFSDFELANRHWMIEYFPKHKSLV
                     SKFTNP"
     gene            complement(1609..2892)
                     /gene="hpnE"
                     /locus_tag="VF870_00045"
     CDS             complement(1609..2892)
                     /gene="hpnE"
                     /locus_tag="VF870_00045"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEX9250598.1"
                     /translation="MNRCLIIGGGLAGLSAAAILSSKKFNVTLIESSPKLGGKTYSFF
                     DKESNSEIDNGQHIMMGCYKETLSFLKLIGAENNFDYQKNLFLKFIDRNKNQYQLDAS
                     FSFYPFNLLFAVVNYDVLNPIEKLSFILFVLRLPFLSKNNLKKMTVLEWLEKENQNPN
                     IIKSFWEILCVGALNTNLEKASAFVFYEVLIQIFFKGNFASTIILPKYGLSKSIIDQA
                     SLFIEKNNGKIILSETVKEVVVKNQKVVCLKSDLKTYDDFDYVISAVPLYSLERIIDK
                     KSLDINVDLNYSTILNIHIWLKENKLKEKFYGLLDSPLHWIFVKETHINIVISDADYL
                     IDKSKEDIFSFVVDELTIYTSIINDDILNYKIIKEKRATFVPTVETISKRPNSNTGIN
                     NLFLAGDWINTGLPSTIESAVKSGSIAAKIVLKKS"
     gene            complement(2892..3734)
                     /gene="hpnD"
                     /locus_tag="VF870_00050"
     CDS             complement(2892..3734)
                     /gene="hpnD"
                     /locus_tag="VF870_00050"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX9250599.1"
                     /translation="MQESAKEISKNSKSSFYYAFNLLPEDKRDAMNTVYAFCRKTDDI
                     VDENSDSTDLKYEKLRKWRIEFEKSFSGHSDFALLNKLGTTISKFNIPLDPFFELIKG
                     MEMDLQKDRYKSFEDLQLYCYRVASTVGLMCIEIFGYKHPSTKKFAVDLGIALQMTNI
                     LRDIGKDAKHGRIYLPQEDLIRFNYSEQEILSLLYNNNFRDLMVYESARAKQYFNSAT
                     ANLDLDDKKTMFAARAMQHIYYKMLENIIAADYDVFNNDIKVSKFEKVGIALGVWAKY
                     NLVY"
     gene            complement(3706..4578)
                     /gene="hpnC"
                     /locus_tag="VF870_00055"
     CDS             complement(3706..4578)
                     /gene="hpnC"
                     /locus_tag="VF870_00055"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX9250600.1"
                     /translation="MDNKSNTSGKKSSILIKDHYENFPVASFLLPKSYRKDIVIVYWF
                     ARTADDFADEGNAKTEERLDELNTFEIEFQKSLNGKSENFHFNQLAGTISNRKLSPKY
                     FIDLLSAFKQDVVKKNYANYDEVLDYCERSANPVGRIMLELFNIKDEQAIISSDKICT
                     ALQLTNFYQDTIIDIEKGRNYYPQDEMKMYNVTQKMFELKKNNPNIKVLVKHNVERTQ
                     TLFDEGKSLLKYLDGRFKIEIKWTIAGGEKILDKIRKNNYDVFTNRPKLSKMDFTLLL
                     VKSFFNARISKRNI"
     gene            4805..5209
                     /locus_tag="VF870_00060"
     CDS             4805..5209
                     /locus_tag="VF870_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9250601.1"
                     /translation="MRAAAEDIEMLLLNDVAIEVVHLSRATYKEAGQLKKRLDELIKS
                     NQKKIIVDISQCEFIDSTFLGVLVLSLKSIAKISGDLRLVKPHKIVKTLIDKAGTLNV
                     FNVYNSTEDAINSFNVNGDQNYYLGNGASILN"
     gene            complement(5297..>7251)
                     /locus_tag="VF870_00065"
     CDS             complement(5297..>7251)
                     /locus_tag="VF870_00065"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEX9250602.1"
                     /translation="GYRSRYSHQTEEAHPGYYKVKLEDDNVLAELTATKRVGFHKYSF
                     PKTDSAHIMLDLTSGIYNYGGKNVWSFVRVENDTLVTGYRQTNGWARTRLLYFVMTFS
                     KPIQSYGLVNKEEMIYKGFWRKFNQNKNFPEQAGHEIVNYFSFKTQDGESILIKFALS
                     AVSTKNAIENLKAEIPDWDFSKVVENAKTKWNNELSKITIEADEERKVNFYTSMYHSF
                     MSPVIYNDINGEYRGLDQNIYKADGFTNYTIFSLWDTYRALHPLYTIIQQQRTNDIVN
                     SMLAHYDQSVHKILPIWSHYSNENWCMIGYHAVPVIVDAYMKGLRNYDVKKALDAVLS
                     SSNYELYDGIGNYKKYGYVPEDLNSNSASKTLEYAYDDWTICKFAEALGKKQIADEYF
                     KRAQSYKNLFDEKTNFIRAKNSDGKFKEPFDALSTANQGYIEGNAWNYSLYIPHDING
                     FINLLGGKDKLISWIDSLFTMQVSDESFAHSEDIDRAGMIGNYVHGNEPSHQVPYMYC
                     YAGVPWKTQERIHQIVNLMYKNKPDGLSGNDDCGQMSAWYIFSVLGFYPVAPGSNQYV
                     IGSPCINNAVINLENGNKFKIETENYSEKNIYIKEIYLNGDLLNRTYIKHDEIVEGGT
                     LKFVMSSTPNMKLGSEDKFYPYSLTR"
BASE COUNT         2364 a         1237 c         1047 g         2603 t
ORIGIN      
        1 tccttctgta gtttcacttc tccatgcacc atgaatttgt actgatccgc caatttttaa
       61 actaatttta ggcgtaccaa aaatgctaag tactcctacg cttggtaatg gtatttcaaa
      121 gtcggtaagg cttttcataa aatcgcctaa acctagttta gagtctttta attcatatga
      181 gtaaccaaga gcctcccatt ttttacgttc ctcaacttcc agccttgtct taatgtattc
      241 atcgatagga attctaagta caatcttatt aggcttacct gcaacaattt cttttatctc
      301 aacatatttg ccggtggaat caattgaaat tattcttgat ttaaaagtcg gggaaggttc
      361 aacaaaaaac ttggattttt tcttttgctt taatgttgtg tatggaacat cttcacgctg
      421 ataccggaag tattgtaatc ttgctgtaga gtctaatgcc atctgcacta accttaaaga
      481 atcctgattt accttaactt tagtttttaa ggaatctttg ccagttactt ttgtggaatc
      541 aagaagatta ctcaaacctg aagaatcttc cggagaaaag attgagtcat caatagcagc
      601 aaagggtgaa tccggtgaga atgaattatc agtaattgat aaggcttcgt tttttttctt
      661 ttcagagtcg ggtttaatta tgccagcatc atactcatta ttataattta aattccgtaa
      721 atcggagatt aaattataac aagcagcctg aagcatagct tcattaacct gagatttata
      781 gccggagcca aacccaagat ttatacctgc gtaaaatatc actaaagaaa ccagaactaa
      841 tttagcagtt tcaataatga tgttttttat tttgcgttta atgcagaatt cctagtaaag
      901 tgaaacaaaa taaatcgtag agtatattct atatggctcc aatattattt ataatttgcg
      961 gcaaaaatta gacaaaatcg tctaataatt caattaaaaa cttaatactt ttaaggtatt
     1021 acaagctatt caaataacta acataaatga aggtattttt ttttcgcaaa cagctgagtc
     1081 acttttatgg attcgtgaat tttgaaacta aacttttgtg ttttggaaaa tattctatca
     1141 tccaatgtct atttgctaac tcgaagtcag agaaatcaaa tccaggtgat actgtacaac
     1201 ctattaatga gaatgatctt ttattaatta cttcagcagc aaaccaatta ttttttttga
     1261 taacagtctg aaatacttct ccatcttctg ttttcttacc caaaataatt tccgtcaatt
     1321 ttcctttttc acctatcacg taaagcttaa cagaaccgcc atcataaaaa tgccatagtt
     1381 catctgattt caatctatga aactttgaaa cctgcgaccc ctcaagtaaa aaataaatcg
     1441 atgttgagaa atttcttttt agattttttt ttggagcttc aattgtgatt atttctcctg
     1501 aacgataaac ttctctaaaa tatccgcctt ccggatgctt aatcaactgt aatttttcta
     1561 tataatattt tgctttttga ttcataatct ttccaatatt tttaagtact agcttttctt
     1621 taatacaatc ttagccgcta tacttccgct tttaaccgcg ctttcaattg tggaaggtag
     1681 tccggtattt atccaatcac cggccaggaa taaattatta attcctgtat tactgtttgg
     1741 tcttttagag attgtttcaa ctgtcggcac aaaagtagct cttttttctt ttatgatttt
     1801 ataattaagg atatcatcat taatgattga agtatatatg gttaactcat caacaacaaa
     1861 actaaatata tcttctttac ttttatctat aagataatca gcatcactga ttacaatatt
     1921 aatgtgtgtt tcctttacaa atatccaatg taaaggagaa tctaacaatc cataaaattt
     1981 ttcctttagt ttattttctt tcaaccagat atgtatgttt aaaattgtgg agtaattcaa
     2041 atcaacattg atatccaaag attttttatc gataattctt tctaaagagt aaagcggaac
     2101 tgctgaaata acataatcaa aatcatcata agtctttaaa tcagatttta aacaaacaac
     2161 tttttgattt tttacaacaa cttcttttac agtttcagat aaaataattt taccattatt
     2221 tttttcaata aataacgatg cctgatcaat tatgctttta cttagtccat atttcggaag
     2281 aataattgtc gaggcaaaat tacctttaaa gaaaatctgg atgagcactt cataaaaaac
     2341 aaaagccgaa gctttttcca aattagtatt taatgcacca acacagagta tttcccaaaa
     2401 acttttaata atgtttgggt tctgattttc tttttccagc cactccaata cagtcatctt
     2461 tttcaaatta tttttactaa ggaatggaag tctcaacaca aataagataa aagataactt
     2521 ctcgatagga ttaagtacat cgtaatttac tacagcaaat aaaagattga atggatagaa
     2581 tgagaatgaa gcatctagct gatactgatt tttatttcta tcaataaatt tcagaaaaag
     2641 atttttctga taatcgaaat tattttcagc accaattagt tttaggaatg aaagtgtttc
     2701 cttgtaacag cccatcataa tatgctgacc gttatcaatc tcagaattcg attctttatc
     2761 aaaaaaggaa taggtttttc cgcctaattt tggcgatgat tcaattaaag ttacattgaa
     2821 tttttttgaa gataggattg ctgcggcact taatcccgct aaaccgccgc cgattatgag
     2881 acaccgattc attaataaac taaattatac tttgcccaaa ctcccaaagc tataccaact
     2941 ttttcaaact ttgagacttt tatatcatta ttaaaaacat cgtagtctgc ggcaataata
     3001 ttttctaaca ttttgtaata gatgtgctgc attgctcgtg ctgcaaacat ggttttttta
     3061 tcatccaaat ccaggtttgc agttgcagaa ttgaagtact gttttgctct ggcagactca
     3121 taaaccatta agtctctaaa attattgttg tagagtaacg ataaaatttc ctgctcacta
     3181 taattaaatc taatcagatc ttcctgaggc aaatatattc ttccgtgctt agcatctttt
     3241 ccaatatcac gcagaatatt agtcatctgc agggcaattc caagatcaac agcaaacttt
     3301 tttgtagatg gatgtttgta tccaaaaatt tctatgcaca ttaatccaac agttgaagca
     3361 acccggtaac aatagagctg aaggtcttca aatgatttat aacgatcttt ctgtagatcc
     3421 atttccatcc cttttataag ttcaaagaat ggatcgagcg gaatattgaa ttttgaaatt
     3481 gtagttccaa gtttgtttaa taaagcaaaa tcagaatgac ccgaaaaact tttttcgaat
     3541 tcaattcgcc atttccttaa tttctcgtat ttaagatctg tggaatcaga attttcatca
     3601 actatatcat ccgtcttgcg gcaaaatgca taaacagtat tcattgcatc gcgtttatct
     3661 tcaggtagaa gattaaaagc ataataaaaa ctgcttttgc tatttttaga tatttctttt
     3721 gctgattctt gcattaaaaa aacttttaac taacaacaat gtaaaatcca ttttactgag
     3781 tttcggtcga ttagtaaaaa catcataatt attttttcta atcttatcca gtattttttc
     3841 accaccggca atcgtccatt tgatttcaat tttaaatctg ccatctaaat attttagcag
     3901 acttttgcct tcatcaaata gtgtttgggt tctttcaaca ttatgtttta ctaacacttt
     3961 aatattagga ttatttttct ttaactcaaa cattttttga gtgacattgt acattttcat
     4021 ttcgtcctga ggataataat tccgaccttt ttctatatca attatagtat cctgataaaa
     4081 atttgtaagc tgcagtgcag tgcaaatttt atctgaactt atgattgcct gttcatcttt
     4141 aatattaaat agttcaagca taattcttcc aaccggattt gcagatcgtt cacagtaatc
     4201 caaaacttca tcgtaattag cataattttt ttttacaaca tcctgcttaa aagcagatag
     4261 taaatcaata aaatattttg gagataattt tctattgcta attgtcccag ctaattgatt
     4321 aaaatgaaaa ttttctgatt tcccatttag agatttctga aattcgattt caaacgtgtt
     4381 taattcatcc aacctttctt ccgttttggc atttccttca tcggcaaaat catctgcagt
     4441 tcgtgcaaac caatatacaa ttacaatatc cttacgataa cttttgggta ataaaaatga
     4501 ggcgacagga aaattttcgt aatgatcttt aattaaaatt gaagactttt tacccgatgt
     4561 atttgattta ttatccaaag aatttctggt caatattatt gaattttgta attccaaaat
     4621 ggaataaaca tgcgctttca aatatcataa tgaggcaaag attttaattt tactttgaaa
     4681 tttaacaaaa ttgataatta aaactatgga acaaatattg tcctgtttaa ctcgatgcgt
     4741 gcttaaacta tttcaaataa aaggggaaat ttaggatgtt caagggaaca agagaggaga
     4801 tttcatgagg gcagcggcag aagatattga aatgcttctt ttaaatgatg tggcaattga
     4861 agttgtccat ttaagccgtg ctacgtataa ggaagcaggt caacttaaaa aaagactcga
     4921 tgaattaatt aaaagcaatc agaaaaaaat catcgtagat ataagtcaat gtgaattcat
     4981 tgattcgaca tttcttggtg tgcttgtatt atcattaaaa tccatagcaa aaattagcgg
     5041 tgatttacga ttagtaaaac cacacaagat tgtaaaaact ttaatagata aagctggtac
     5101 tttaaatgtt tttaacgttt ataattcaac agaagatgca ataaacagtt ttaatgttaa
     5161 tggcgatcaa aattattatc ttggtaatgg tgcttcgata ttaaattaaa acaatgaaag
     5221 ccaattttgg gaattgtcct tcgttggtta gagaacctct cgtaattgag aggtttttta
     5281 ttttaaatct ataattttac ctggttaaag aatatggata aaatttatct tcacttccca
     5341 atttcatatt aggagttgaa gacataacaa attttaaagt tcctccttca acaatttcat
     5401 cgtgtttgat gtatgtgcga tttagtaaat caccattaag ataaatttct tttatgtaaa
     5461 tatttttttc agaataattt tctgtttcaa ttttaaattt atttccgttt tccaggttga
     5521 ttacagcatt gtttatacat ggacttccaa tcacatactg attactgcca ggtgcaaccg
     5581 gataaaagcc taaaacacta aaaatgtacc aggcggacat ttgtccgcaa tcatcattcc
     5641 cggataatcc atcaggttta tttttgtaca ttaagtttac tatctgatga attcgttcct
     5701 gagttttcca tggaactccg gcataacaat acatataagg aacctgatga cttggttcat
     5761 tcccatgaac ataatttcca atcataccgg cacgatcaat atcttcgcta tgagcaaagc
     5821 tttcgtctga aacttgcatt gtaaaaagcg aatcaatcca actgatcagt ttatctttgc
     5881 cgccaagcag atttataaaa ccatttatat cgtgcggaat ataaagtgaa taattccagg
     5941 cattaccttc aatgtaacct tgattcgcag tacttaatgc atcaaatggt tctttaaact
     6001 taccatcaga gttttttgct ctgatgaaat ttgttttttc atcaaaaaga tttttatacg
     6061 attgtgcccg tttaaaatac tcatccgcta tttgtttttt tccaagtgct tctgcaaact
     6121 tgcagatagt ccaatcatca taagcatatt ctagtgtttt agaggctgag tttgagttta
     6181 aatcttccgg cacatagccg tattttttat agttaccaat tccatcatac aattcataat
     6241 tagaacttga tagaacagca tccagtgctt ttttaacgtc gtagtttcta agtcccttca
     6301 tataagcatc aacaataacc ggaacagcgt gataaccaat catacaccaa ttttcatttg
     6361 agtaatgaga ccagatgggt aaaattttat gaacactttg atcataatgt gctaacattg
     6421 aatttacaat atcatttgta cgttgctgct gaataatagt atatagcgga tgaagggcgc
     6481 gatatgtatc ccacaaagaa aagattgtat aattcgtaaa tccatcagct ttgtaaatgt
     6541 tttgatcaag accgcgatat tctccattaa tatcattgta aattacggga ctcatgaaac
     6601 tatgatacat tgaagtgtag aaatttactt ttcgttcctc atcggcttcg attgttatct
     6661 tcgaaagttc gttgttccat ttggtctttg cattttctac aactttagaa aaatcccaat
     6721 caggtatttc tgctttgaga ttttctattg cattttttgt acttactgcg gataaagcaa
     6781 attttattag tattgattct ccatcttgag ttttaaagct aaaataattt acgatttcat
     6841 gtccggcttg ttcaggaaaa tttttattct gattaaactt tctccaaaag cctttataga
     6901 tcatttcttc tttattaacc aaaccataag attgaattgg ttttgaaaaa gtcattacaa
     6961 agtaaagaag tcgtgtcctt gcccagccgt tcgtctgtct gtaacctgta acaagcgtgt
     7021 cattttcaac ccttacaaaa gaccaaacat tttttccacc ataattataa atgcctgaag
     7081 ttaaatcgag cataatatgt gctgaatcag tcttagggaa actgtattta tgaaatccaa
     7141 ctcttttagt tgcagttaat tcagcaagaa cattatcatc ctctaatttt actttataat
     7201 aaccgggatg tgcttcttct gtttgatgtg aataacgaga tcgataacct g
//
LOCUS       DASWBY010000005         9543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_338609, whole genome shotgun sequence.
ACCESSION   DASWBY010000005 DASWBY010000000
VERSION     DASWBY010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9543
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338609"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VF870_00070"
     CDS             <1..552
                     /locus_tag="VF870_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX9250603.1"
                     /translation="YEISMGFSIGGVILLSESLRPMAIVESQSGLMWNAFIQPIGFLT
                     FLVSAFAETNRLPFDLPEAEPELVGGYHTEYSSMKFAAFFLAEYANMLVASTLIVTLY
                     LGGWQIPYIETFGLSSGLVLALQVAGFFVKVGVLLFFFIWVRWSIPRFRYDQLMNLGW
                     KVMFPLSLFNIIWVAVLIMIFKL"
     gene            604..1113
                     /gene="nuoI"
                     /locus_tag="VF870_00075"
     CDS             604..1113
                     /gene="nuoI"
                     /locus_tag="VF870_00075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX9250604.1"
                     /translation="MSGNNIPPKKRIKDLTFGEKIYIPEIVKGLSLTLRTMFKPKYTM
                     EYPEVKFIPPQSYRGRPVLVEEKNGEERCVACGLCSRVCPALAIEVQAAETDREKERY
                     PEKFEINMLRCIFCGFCEEVCPEEAIVMSKDYELAFSNREDAIYGKDKLLVPVDQLQD
                     RIDFLRNYR"
     gene            1221..2759
                     /locus_tag="VF870_00080"
     CDS             1221..2759
                     /locus_tag="VF870_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX9250605.1"
                     /translation="MLSKTLTSATYGIDAYLVEVETHVEKQIPGFIIVGLPDNAVRES
                     RERVIAAIKNSDIKFPLKKFTINLAPADIKKEGSSFDLAIAIGILAAEELVSDKFLSD
                     SVFLGELSLDGTLRHIKGALPIAVESRKKGIKRIIVPKESANEAAIVDGIDVYGFENL
                     TEVISFLNEDSIPEKTITDKNDLFSSINSYLHDFSDVKGQENVKRALEVAAAGAHNIL
                     MIGPPGSGKTMLAKRLPSILPPLTFDEALETTKIHSIAGILPKHKAIITERPFRSPHH
                     TVSDAALVGGGSFPKPGEVSFAHFGVLFLDELPEFKKNVLEVLRQPLEDARVTVSRSK
                     LSLEFPANFMLAAAMNPCPCGFYTDPNKECTCAPPQIQRYMAKISGPLLDRIDIHIEV
                     PAVKYKELSSKSFGEKSEDIRKRVIVARQIQLERFKDHKNVFNNGDMGSKEVRTFCKL
                     DEQGEELLKMAMTKLGLSARAYDRILKVSRTIADLENSQNILPQHISEAIQYRSLDRE
                     LWKH"
     gene            3037..3120
                     /locus_tag="VF870_00085"
     tRNA            3037..3120
                     /locus_tag="VF870_00085"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3072..3074,aa:Tyr,seq:gta)
     gene            3186..3259
                     /locus_tag="VF870_00090"
     tRNA            3186..3259
                     /locus_tag="VF870_00090"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3220..3222,aa:Gly,seq:tcc)
     gene            3321..3393
                     /locus_tag="VF870_00095"
     tRNA            3321..3393
                     /locus_tag="VF870_00095"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3354..3356,aa:Thr,seq:ggt)
     gene            3475..3639
                     /gene="rpmG"
                     /locus_tag="VF870_00100"
     CDS             3475..3639
                     /gene="rpmG"
                     /locus_tag="VF870_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX9250606.1"
                     /translation="MAKSNIRQIIVLESTAGTGYRYSTTKNKRTHPARVEYKKYDPVV
                     RKHVIFKETK"
     gene            3645..3717
                     /locus_tag="VF870_00105"
     tRNA            3645..3717
                     /locus_tag="VF870_00105"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3678..3680,aa:Trp,seq:cca)
     gene            3738..3920
                     /gene="secE"
                     /locus_tag="VF870_00110"
     CDS             3738..3920
                     /gene="secE"
                     /locus_tag="VF870_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX9250607.1"
                     /translation="MKDKIIAFFQDVVKEMKKVTWPTKEELIESTKIVIVVCIVLSLF
                     TYVIDMIINQAFKGIF"
     gene            3932..4468
                     /gene="nusG"
                     /locus_tag="VF870_00115"
     CDS             3932..4468
                     /gene="nusG"
                     /locus_tag="VF870_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX9250608.1"
                     /translation="METRWYVVRTFSGHETKVKNLLEAGLVDHENLRAKISDILVPME
                     KVFEVKDGKKKSKTKNFFPGYILVNADLDNQVKDFILSTPSVMGFLGTGNNPNPLQAE
                     EVRRIVGRLSQDSTTERMETIFRSGDLVKIIDGPFNNFSGTIEEVNEEKMKIKVMVSI
                     FGRKTPVEIDFTQAEIEK"
     gene            4495..4920
                     /gene="rplK"
                     /locus_tag="VF870_00120"
     CDS             4495..4920
                     /gene="rplK"
                     /locus_tag="VF870_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX9250609.1"
                     /translation="MAKKIDSYIKLQIPAGKANPSPPVGPALGQKGVNIMEFCKQFNA
                     RTQDKDGLIIPVVITVFSDKSFTFITKTPPASVLLKRAAKLEKGSAESHRTKVGKVSS
                     KQVKEIAEVKMPDLNAFDIDHAMSMISGTARSMGITVEE"
     gene            4964..5662
                     /gene="rplA"
                     /locus_tag="VF870_00125"
     CDS             4964..5662
                     /gene="rplA"
                     /locus_tag="VF870_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX9250610.1"
                     /translation="MQTTKRNKEILKSVNVDKEYTLPEAISILKDQSKVKFVESLDCA
                     IRLGVDPRHADQMVRGTVSLPHGTGKTVKVLVIAKGAKIQEALDAGADHAGFEDYLEK
                     IKGGWADVDVIVSTPDSMAELGKLGKILGPKGLMPNPKSGTVTMDVAKAVKEVKGGKI
                     EFRVEKTGIVHTSLGKLNFETDKLVDNTKAFLNTIIKMKPSSSKGQYVKSLFLSSTMG
                     PGLKISKDELSLNV"
     gene            5737..6258
                     /gene="rplJ"
                     /locus_tag="VF870_00130"
     CDS             5737..6258
                     /gene="rplJ"
                     /locus_tag="VF870_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX9250611.1"
                     /translation="MNKNEKAELIAEAKELIQNATALYLTDYSKINVADISDIRNQFR
                     KEGVKYRVFKNTLFKRALVESGKFEKLADHLEGMTGFAFASTNPVAPAKIIKKYNDAS
                     QKFPLKACYIETQYYDGSKLKELAELPTKEEVIAGILGSLNSPASGIVGSIAAVIRNL
                     VSVVDEVSKKKAA"
     gene            6292..6666
                     /gene="rplL"
                     /locus_tag="VF870_00135"
     CDS             6292..6666
                     /gene="rplL"
                     /locus_tag="VF870_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX9250612.1"
                     /translation="MSEKVAQALELIKGMSLLEASDLKKALEEEFGVTASAPVMMAGP
                     AAASAAPVEEQTEFDVVLQAAGEKKINVIKVVRAHTGLGLKEAKDLVDGAPKTVKEQA
                     SKDEAEKIKKELEEAGATVTLK"
     gene            6802..>9543
                     /gene="rpoB"
                     /locus_tag="VF870_00140"
     CDS             6802..>9543
                     /gene="rpoB"
                     /locus_tag="VF870_00140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9250613.1"
                     /translation="MDNNRISFGRIPSVIDIPDLLGIQTETFEDFVQLDTHPLKRENK
                     GLQQVFLTNFPIFDNKENYRLDFLEYYVEKPRFSVEECLERGLTFAAPLKAKLRLSTK
                     DPETEEFINTVEQEVYLGNLPFMTEKGTFVINGAERVVVSQLHRSPGVAFAQTVHPNG
                     TPIYSARIIPLRGSWVEFATDINYVMYVYIDRRKKFPATTLLRALGFQSDEEILKLFD
                     LVENVSVKKAKLENYLGRMIASDIFDMSTGEIFLTRDSILSEEDLERINDAEVDVLKF
                     IKSDSSNEQNLVVNTLRKDTSHTREEALYAIYRQLRSGEAPDLTTAETLIEKLFFNDK
                     RYDLGDVGRHRMNHKLELNIPETTTVLTVEDIISIMKYIIDLKEGAVSVDDIDHLGNR
                     RIRTVGEQLGQQFNVGMARMARTIKERMNMRDTENFTPQDLVNARTISSVINAFFGTN
                     QLSQFMDQTNPLAEMTHKRRMSALGPGGLTRERAGFEVRDVHYTHYGRLCPIETPEGP
                     NIGLISSLTIYARVNRYGFLETPYRKVVKGRVTEDVEYLTAEHEDAFTIAQANAPITE
                     SGKFINERVKSRLKGEFPIVHPEDLQFMDVAPAQIVSAAAALIPFLEHDDANRALMGS
                     NMQRQSVPLLKPEAPIVGTGMEGKIAHDSRALLLAEDNGVVEYVDANKIIVKYDINPN
                     SFEALTSFNEIREVTYTLTKFHGTNQETCINQVPIVKEGQKLKRGDVLADGAATDGGE
                     LALGRNVLVAFMPWRGYNFEDAIIISERVVSEDVYTSIHIEEFELQVRETKRGEEELT
                     REIPNVSEEAVKNLDEDGIIREGAEVKEGDILIGKITPKGETDPTPEEKLLRAIFGDK
                     AGDVKDASLKAPPGLKGTVIKTRLFSRKKRDNDSKKLEKIAFDKLEQEFKNKLHDHYQ
                     "
BASE COUNT         3307 a         1480 c         1892 g         2864 t
ORIGIN      
        1 tatgaaattt cgatgggatt ttctatcggc ggtgtaattc ttttatcaga atcattaaga
       61 ccgatggcaa tagtagaatc acaatccggt ttaatgtgga acgcatttat acaaccaata
      121 ggatttttaa cttttcttgt atcagcattt gcagaaacta acagattacc atttgatcta
      181 ccggaagcag aaccagaact tgtaggtggc tatcacactg aatacagcag tatgaagttt
      241 gctgcattct ttttagcaga gtacgcaaac atgctggttg caagcacact tattgtaacg
      301 ctgtatcttg gcggatggca aattccttac attgaaactt ttgggttatc atcaggatta
      361 gttttagcac tccaggttgc agggtttttt gtaaaagttg gagtattact attcttcttt
      421 atctgggtaa gatggagcat tccaagattt aggtatgatc agctaatgaa tttaggatgg
      481 aaagtgatgt tcccattatc gttgtttaac attatttggg tcgcagtttt aatcatgatc
      541 tttaaattat aatttaatat tgttcaatta tgaataagct agaaaatatt aacaggagtt
      601 ttgatgtcag gtaataatat tcctcctaag aagagaatta aagatttaac atttggcgaa
      661 aagatttaca tcccggaaat tgttaaagga ttatcgctta ctttaagaac gatgttcaaa
      721 ccaaagtaca ctatggagta tcctgaagta aagttcattc cacctcaatc ttatcgcggc
      781 agacctgtgc ttgttgaaga aaagaatggt gaagaaagat gtgttgcttg cggattatgt
      841 tcacgtgttt gccctgcact tgctattgaa gttcaggctg cagaaaccga tagagaaaaa
      901 gaaagatacc ctgaaaagtt tgagataaat atgctgcgct gcatcttttg cggcttctgt
      961 gaagaagttt gtccggaaga agcaatcgtt atgagtaaag attatgagtt agctttctca
     1021 aacagggaag atgcaattta tggaaaagac aagcttttag tccctgttga tcaattgcag
     1081 gatagaattg attttttaag aaattataga taaaattttt ttaattattt tttcttacat
     1141 ttaagattta ttgcagcttg gggtggacaa tctcgcctca attacacata ttaaatccaa
     1201 taataaacct ggattaaatc atgttatcca aaactctgac aagtgcgaca tacggaattg
     1261 atgcctatct cgttgaggtt gagactcatg ttgaaaaaca gattcccgga tttataattg
     1321 ttggtttgcc tgataacgct gttagagaaa gccgtgaaag agttattgca gcaataaaaa
     1381 attcagatat aaaattcccg ctcaaaaaat ttacaatcaa tcttgcacct gctgatatta
     1441 aaaaagaagg aagttctttt gatcttgcca tcgcgatagg aattcttgca gcagaagaac
     1501 tagtctcaga taaattttta tctgattcag tttttcttgg tgaactttct ttagacggaa
     1561 ctttaagaca cataaaaggt gctctaccca tagctgttga atcaagaaaa aaaggaatta
     1621 aaagaattat tgtccctaaa gaatctgcaa atgaagctgc tatcgttgat ggaattgatg
     1681 tttatggatt tgaaaattta accgaagtaa tttcattttt gaatgaagac tccattcctg
     1741 aaaaaactat tactgataaa aatgatttgt tctcatcaat aaattcttat ttgcatgatt
     1801 tttcagatgt gaagggacag gaaaatgtta agcgcgcatt ggaagtcgct gctgccggtg
     1861 cacacaatat tttaatgata ggtcctcccg gctcaggtaa aacaatgctt gcaaaaagat
     1921 taccttctat acttccgccg cttacatttg atgaagcact tgaaacaaca aagattcatt
     1981 caatcgccgg catcttaccg aaacacaaag caattattac agaaagacca tttagaagtc
     2041 cgcaccacac tgttagcgat gcggctttag ttggtggcgg atcatttcca aaaccaggtg
     2101 aagtttcttt tgcacatttt ggagttttat ttttagatga attaccggag ttcaaaaaaa
     2161 atgttttaga agttttgcgt caaccgcttg aagacgcaag agtaacagta agtcgttcaa
     2221 aattatcttt ggaatttcct gcaaatttta tgttagccgc tgcgatgaac ccctgtccat
     2281 gcggttttta tactgatcca aataaagaat gcacctgtgc accaccgcaa attcaaagat
     2341 acatggcaaa aatttccgga cccttgttag atagaataga tattcatatt gaagttccag
     2401 cagttaagta caaggaattg tcctcaaaat catttggaga aaaatctgaa gacattagga
     2461 aacgagttat tgttgcaaga caaattcaat tagaaagatt taaagatcac aaaaatgttt
     2521 ttaacaatgg cgatatgggc tcaaaggaag ttagaacttt ttgcaagtta gatgagcaag
     2581 gcgaagaatt attgaaaatg gcaatgacaa agttgggatt atctgcaaga gcatatgata
     2641 gaatactaaa agtcagcaga actatcgcgg atcttgaaaa ctcacaaaat attttacccc
     2701 agcatatcag tgaagcaata caatatcgaa gccttgatag agaactttgg aagcactaaa
     2761 attataagcc tatgctaaag tatattttta atatgttttc aattataaga ataagttgca
     2821 aaatctatca caaatcggtt ttttaggcta aatttagaat ttcatcactc attctcattt
     2881 ccaaataagt agttaaaacc cggatattta agagtcggta ataataacga gatctgtttt
     2941 gttaattaat cgcagaatta gttataatat aaaatatttg catttttgtt tgaataaaaa
     3001 tatatttgtc gctcgctttt attttagcat tattctgggc ggacgccgga gttggagagc
     3061 cggggcggac tgtaaatccg ttggcatgcg cctttggggg ttcgaatccc tcgccgccca
     3121 catatagatt caacattagt ttcttcttag aaactttttt gttcttaagg atttttgaaa
     3181 tttttgcggg agtaactcag ttggctagag tcacagcctt ccaagctgtt ggtcgcgggt
     3241 tcgaatcccg tctcccgctc tagacaaatt agacaaaatt gctgattttg ttctttaatt
     3301 taatgtgtta tcacatctcc gctgacgtag ctcagttggt agagcacatc cttggtaagg
     3361 atgaggtcac cggttcaatc ccggtcgtca gctcaataag tttaatgagg tcattccggt
     3421 ataatcggaa taaacagctt aatagaaaag gtttgaatag tttaaggaaa tcagatggca
     3481 aaaagcaata ttagacaaat cattgttttg gaaagcacag caggaacagg ttacagatat
     3541 tcaactacaa agaataaaag aacccatcca gcacgtgtag agtacaaaaa atatgaccct
     3601 gttgtcagaa aacacgtaat ttttaaggaa accaaataat taatacgtca gtggctcaat
     3661 tggtagagca gcggtctcca aaaccgcagg ttgggggttc gagtccctcc tgacgtgctg
     3721 aaatttgaaa ggttattatg aaagataaaa ttattgcatt ctttcaggat gttgtaaaag
     3781 agatgaaaaa agttacctgg cctacaaaag aggagcttat tgaatctact aaaattgtaa
     3841 tagttgtttg tattgttcta tctttattta cttatgtaat tgatatgatt attaatcagg
     3901 cttttaaagg gatattttag gtgcgtaact aatggaaacc agatggtatg tagttagaac
     3961 attttcagga catgaaacta aagttaaaaa tctcttagag gcaggtttag ttgatcacga
     4021 aaacctaaga gctaaaattt cagatatttt agtgccgatg gaaaaggttt ttgaagtaaa
     4081 agatggcaag aaaaagagca aaacaaagaa cttttttccg ggatatattt tagttaatgc
     4141 agatttagac aatcaggtta aagattttat actaagtact ccttccgtaa tgggattttt
     4201 aggtacaggt aataatccta atccgctgca agctgaagag gttagaagaa tagtgggcag
     4261 actttctcaa gacagtacaa cagaaagaat ggaaacaatc ttcagaagtg gcgatcttgt
     4321 taaaattatt gatggtccgt tcaacaactt ctcgggtacg attgaagaag tgaacgaaga
     4381 aaaaatgaaa ataaaagtaa tggtttcaat ttttggaaga aaaactccgg ttgaaattga
     4441 ttttactcag gctgaaatag aaaaataatt attggttatt aattaggttg taaaatggct
     4501 aaaaaaattg attcatacat aaaattacaa attcctgctg gtaaagctaa tccttcacct
     4561 ccagttggtc cggcacttgg tcaaaaaggt gttaatatca tggagttctg caagcaattt
     4621 aatgcaagaa cccaggataa agacggattg ataattcctg ttgttatcac agtatttagt
     4681 gataaatctt ttacttttat aactaaaacc ccaccggctt cagttctttt gaaaagagca
     4741 gcaaaacttg aaaaaggatc tgcggaatct cacagaacaa aagttggaaa agtatcttct
     4801 aaacaggtta aagaaattgc agaagtaaaa atgccggatc ttaatgcatt tgatattgat
     4861 catgcaatga gtatgatttc cggaacagct agaagtatgg gaataactgt agaagaataa
     4921 ataagtattt atttgatgtt tttaaaattt atcggtgatt aagatgcaaa caacaaaaag
     4981 aaataaagag attttaaaat ctgttaacgt agataaagaa tatactctac ctgaagcaat
     5041 ttcaatctta aaagatcagt caaaagtaaa atttgtggag tctttagatt gcgccatcag
     5101 attaggcgtg gatcccagac acgctgacca aatggtaaga ggtaccgttt ctttaccgca
     5161 cggaactggt aaaacagtta aagttcttgt tattgctaaa ggtgcaaaaa tacaagaagc
     5221 tttagatgcc ggagccgatc acgcaggatt tgaagattat ttagaaaaga ttaagggtgg
     5281 atgggcagat gttgatgtaa tcgtttcaac acctgattct atggctgagc ttggaaaact
     5341 tggtaagatt ttaggtccaa aaggtctgat gccaaatcct aagagtggta cagttactat
     5401 ggatgtggca aaggcagtta aagaagttaa aggtggaaaa attgaattca gggttgaaaa
     5461 aaccggcata gttcatactt ctttaggaaa actaaacttt gaaacagata aacttgttga
     5521 taatacaaaa gcttttctta acactatcat caaaatgaaa ccttcctcat cgaagggaca
     5581 atatgttaag agtttgtttt tatcaagtac aatgggaccc gggcttaaaa tctctaaaga
     5641 tgaattaagt ctaaacgtat aaaaagaatt tacgcgctca taataaataa tagcttctaa
     5701 ccgacccgaa ataataaaat gtgatacggg agaaaaatga ataagaacga aaaagcagag
     5761 ttaattgccg aagctaagga attaatccag aacgcaacag cactctatct aactgattat
     5821 tctaaaatta atgttgctga cattagcgat attagaaatc agtttagaaa agagggagtt
     5881 aaatacagag tatttaaaaa taccttattt aaacgcgctc ttgtagaatc tggtaaattc
     5941 gaaaagctgg cagatcatct tgaaggtatg accggatttg catttgcttc aacaaatcct
     6001 gttgctccag ccaagataat taagaagtac aatgatgctt cacaaaaatt tcctcttaaa
     6061 gcttgctata tcgaaactca atattacgat ggaagtaaac ttaaagagct agcagaattg
     6121 cctacaaaag aagaagtgat tgccggtatt ctcggtagtc taaattcacc tgcgtcaggc
     6181 atagttggat caattgccgc tgtaattaga aacttagtta gcgtagttga cgaagtgtct
     6241 aagaaaaaag cagcctaaat aaattagaaa ttaaattcag gagaaatgaa aatgtcagaa
     6301 aaagttgcac aagcactcga actaataaaa ggaatgtctc ttctagaagc ttcagattta
     6361 aagaaagcac ttgaagaaga atttggtgtt acagcatctg caccagttat gatggctgga
     6421 ccagctgctg catccgctgc accggttgaa gaacaaacag aatttgatgt tgttctgcaa
     6481 gctgcaggcg agaaaaaaat taacgtcatt aaagttgtta gagctcatac aggtctaggc
     6541 ttaaaagaag ctaaagatct ggttgatggt gctccaaaaa cagttaaaga acaagcatca
     6601 aaagatgaag ccgaaaaaat caagaaagaa cttgaagaag ccggcgcaac tgtaacttta
     6661 aagtagttat taaaattaag tttatatgat cttttttaag tgataagacg gaaaatgccg
     6721 tcttatcgct tttgttttat atagaaacgt tgtttaggat gctcatttta aagcatttga
     6781 taatttttag gaggttctca attggataat aaccgtattt cctttggccg tattccatca
     6841 gtcatagata tacccgatct tttaggaatt caaactgaaa catttgaaga ttttgttcag
     6901 cttgatactc atcctcttaa aagagaaaat aaagggttac agcaggtttt cttaaccaat
     6961 tttccgattt ttgataataa agaaaattac agacttgatt ttttagaata ttatgtagaa
     7021 aaaccaagat tctctgttga agaatgtttg gagagaggtc ttacttttgc agctccgctt
     7081 aaagcaaagc taagactttc aacaaaagat cctgaaactg aagaatttat taatactgtt
     7141 gaacaagaag tttatcttgg caacctgcct ttcatgaccg agaaaggaac ttttgttatc
     7201 aacggtgcag agagagtagt tgtatcacaa ttacatagat ctcctggtgt tgcttttgca
     7261 cagactgtac acccgaatgg tacccctatt tactcagcac gaatcattcc tttaagagga
     7321 tcgtgggttg agtttgctac tgatataaat tacgttatgt acgtttatat cgatcgccga
     7381 aagaaatttc cggcaacaac attattacgt gcgcttggtt ttcaatccga cgaggaaatt
     7441 ttaaaattat ttgatttggt tgaaaacgtt tcagttaaaa aagcaaagct ggaaaattat
     7501 ttaggtagaa tgattgccag tgatattttt gatatgtcaa ccggtgaaat tttcttaaca
     7561 cgcgattcaa tattatctga agaagatctt gaaagaataa atgatgctga agttgatgta
     7621 ttaaaattta tcaaatctga ctcatccaac gaacaaaatc tagtagtcaa tactttaaga
     7681 aaagatactt cacacactcg tgaagaagct ttgtatgcta tttacagaca gttaagatcc
     7741 ggtgaagctc ctgatttaac taccgctgaa actctaatcg aaaaattatt ctttaatgat
     7801 aaacgatatg atttaggtga tgttggtcgc catagaatga atcataaact ggaattaaat
     7861 attccggaaa caacgacggt tttaactgtt gaagatatta tttctattat gaaatatatt
     7921 atcgatctta aagaaggtgc agtttcagtt gatgatattg atcatcttgg taatagaagg
     7981 attaggactg ttggtgaaca attaggacag cagtttaacg ttggtatggc tcgtatggca
     8041 agaaccataa aagaacgaat gaacatgcgt gataccgaaa attttactcc gcaggattta
     8101 gtaaatgcca gaaccataag cagcgtaata aatgctttct ttggaacaaa tcagctttca
     8161 caatttatgg atcaaacaaa tccattagct gagatgacgc ataaaagaag aatgtctgca
     8221 ttaggacctg gcggtcttac aagagaacga gctggatttg aagtgcgtga cgttcattac
     8281 acacattacg gccgtttatg tcctattgaa actcctgaag gtccgaacat cggtcttatt
     8341 tcctctctta caatttatgc aagagtaaat agatatggat ttcttgaaac accttataga
     8401 aaagttgtaa aaggaagagt aacagaggat gttgaatatt taactgctga acatgaagac
     8461 gcatttacaa ttgcacaggc aaatgcacct attactgaat ctggaaaatt cattaacgaa
     8521 agagttaagt caagattaaa aggtgagttt ccaattgtgc atcctgaaga tttgcaattt
     8581 atggatgttg cacctgccca gattgtaagt gctgcggctg cattgattcc attccttgag
     8641 catgatgatg ctaacagagc attgatgggt tctaacatgc agcgtcaatc agttccattg
     8701 ttaaaaccag aagctccgat tgtcggaact ggtatggaag gaaaaattgc tcacgattca
     8761 agagcattat tattagcaga agacaatggt gttgttgagt atgttgatgc taataaaata
     8821 attgtaaagt acgatattaa tcctaatagc tttgaagcat taacaagctt taatgaaata
     8881 agagaagtaa cttatactct tacaaaattc cacggcacaa accaggaaac ttgtataaat
     8941 caggttccta tcgttaaaga gggccagaaa cttaaaagag gtgatgttct tgctgatggt
     9001 gcagccactg acggcggcga attagcattg ggaagaaatg ttttagtagc atttatgcct
     9061 tggagaggtt ataattttga ggatgctatt attataagcg aaagagttgt aagtgaagat
     9121 gtttatactt caatacacat tgaagaattt gaactacagg ttagagaaac gaaacgtggt
     9181 gaagaagaat taactcgtga aattccaaac gtaagtgaag aagctgtaaa aaatcttgat
     9241 gaagatggta ttattagaga aggcgctgaa gttaaggaag gtgatattct tataggaaaa
     9301 attactccta aaggagaaac cgatcctaca cctgaagaaa aattattacg cgcaatcttt
     9361 ggagataaag ccggtgatgt gaaagatgct tctttgaaag cgcctcctgg acttaaagga
     9421 acagttatta aaactcgttt attcagtcgt aagaaacgag ataatgattc aaagaaatta
     9481 gaaaaaatcg cttttgataa acttgaacaa gaattcaaaa ataagcttca tgatcattat
     9541 caa
//
LOCUS       DASWBY010000006         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_225829, whole genome shotgun sequence.
ACCESSION   DASWBY010000006 DASWBY010000000
VERSION     DASWBY010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225829"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VF870_00145"
     CDS             complement(<1..417)
                     /locus_tag="VF870_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX9250614.1"
                     /translation="MKLKVLVADKFPDKYIQQMKDLDLEVIYNPKLGEKDLPEAAQDV
                     DIVVVRSTVVNEETINHSKKLNLIIRAGSGVNNINIAAANKKGIYVTNCPGMNAVAVA
                     ELAIGLMISIDRSIPDNVSDFDKGVWNKDKYSKGKGL"
     gene            complement(554..898)
                     /locus_tag="VF870_00150"
     CDS             complement(554..898)
                     /locus_tag="VF870_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250615.1"
                     /translation="MNKRFHFSFEMTLLIILFLSVTSLSQDKSKIDFETCTYKDFPLY
                     GKVKFVESFPDIKVQIVDAFPDLKVQLVNSFPDDCGKWQIVESFPDVKIKIVDSFPDI
                     KIKFVESFPGKP"
     gene            complement(1031..>1524)
                     /locus_tag="VF870_00155"
     CDS             complement(1031..>1524)
                     /locus_tag="VF870_00155"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559651.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX9250616.1"
                     /translation="IDMAQKKVHFQGLPARICWLGYGERAKMGKIFNQLVAEGKVKAP
                     IVIGRDHLDCGSVASPNRETEGMIDGSDAIADWPILNALLNAIGGASWVSVHHGGGVG
                     IGKSIHAGMVVVADGTKEAEARLERVLTYDPGMGVMRHADAGYQQAIDNAKKWDVKIP
                     MLK"
BASE COUNT          448 a          335 c          226 g          515 t
ORIGIN      
        1 taatcctttg cctttggaat atttatcttt gttccaaact cctttgtcaa aatcagaaac
       61 gttatcagga atcgatcgat cgatcgagat cattaatcca atcgcaagtt ctgcaaccgc
      121 caccgcgttc atacccggac agttagttac ataaattcct tttttatttg ctgcagctat
      181 gttgatattg ttcactcccg aaccggcacg aattattaaa ttaagtttct tactgtggtt
      241 aatagtttct tcatttacaa cagtagatcg aactacaacg atatcgacat cttgtgctgc
      301 ttcaggaaga tccttttctc caagttttgg gttgtagatt acttcaagat caagatcttt
      361 catttgctgg atatatttat ccggaaattt gtctgctact aatactttga gtttcatatt
      421 aacctctttg taattattta tattcaattg tcatcctaag cctgtcgaag gacgactcac
      481 agttttatta gactaaattc aaacttcctc cctctgcgct cgggataacg ttactatgtt
      541 gctgtgtcta atgctaaggt ttacccggaa acgattctac aaattttatt tttatatctg
      601 gaaaagaatc cactattttt attttgacat ccgggaatga ctcaacaatc tgccatttac
      661 cacaatcatc cggaaaagaa tttacaagtt gaactttaag atcagggaat gcatccacaa
      721 tttgaacttt tatatccggg aatgattcta caaacttaac ttttccataa agcggaaaat
      781 ctttataagt acaagtttca aaatcaattt tgcttttgtc ttgagatagg gaagtaacac
      841 ttaaaaataa aataattaat aaagtcattt cgaacgaaaa gtgaaatctt ttgttcaatt
      901 ttgcaaatag gcttttatat cgaggaatct cttcaactga cataataatt tatttcatct
      961 tacttttctg aatcagactt cttattaaaa ataaatgtca ctcttcgact tcgctcagag
     1021 tgacgtaaat ttattttaac atcggaattt ttacatccca tttttttgcg ttatcaatag
     1081 cctgctgata acctgcatca gcgtggcgca taacacccat accaggatca taagtaagca
     1141 ctctttctaa tctggcttct gcttcttttg ttccgtctgc aactacaacc attccagcgt
     1201 gtatagattt accaatccca actccaccgc cgtgatgcac agaaacccag cttgcaccac
     1261 ctattgcatt tagcaaagca tttaatattg gccaatcagc tattgcatca ctgccgtcaa
     1321 tcattccttc agtttctctg ttcggtgaag caacagatcc gcaatcaaga tgatctcttc
     1381 caatcacaat tggagctttt acttttcctt cagcaacaag ctgattaaaa atctttccca
     1441 tctttgcacg ttcgccataa ccaagccagc aaatccgggc aggcaatccc tgaaagtgaa
     1501 ccttcttctg tgccatatca atcc
//
LOCUS       DASWBY010000007         4226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_263410, whole genome shotgun sequence.
ACCESSION   DASWBY010000007 DASWBY010000000
VERSION     DASWBY010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263410"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="VF870_00160"
     CDS             complement(<1..1370)
                     /locus_tag="VF870_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX9250617.1"
                     /translation="MEPNTLNLIDGAPIAILTFNKNGEIDYANKSFADLELLYGFEYP
                     ANLMGSSIFGKELFPKFTITTELKDILSGLPFEKELNFVETKTQGLIHLIIKGSPIFD
                     GEEIIGGLIIVEDLKILSKTKKESAIRNDFIENAIHNVSDFFLVVDKLERVQFFSKNI
                     LHYINVSVNLIQNVSLGSLFDLETSKVVKEYFEYAKKNRIVLTEKLSYEIRGIRKVFE
                     CRFVPQENFRKEISFVYLFFKDITVQEREIDNLYKEVGTLELYQTITNKSSEAVFVIN
                     DDNIIEYWDDCAEKLFEVSKAEALGVTAQNVVSILSDDFLDSLKSKLIDKEIHKIILS
                     YFDKNRDKKTYETYFTFLDEKKKRIVVKSVDITDKLHFEEGLKLSLKGLHEIISKSPL
                     MICNIDKDGLILFANFAFQKILGYTEEELLSKSFYDLIEPSYFKSNIFDLQSFTGDEI
                     KSLNLPL"
     gene            1692..2465
                     /locus_tag="VF870_00165"
     CDS             1692..2465
                     /locus_tag="VF870_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9250618.1"
                     /translation="MISAAFEKLGNRTLAFLQEFGQISNLFFGIIKSFPQIPKSKKLI
                     LFQMEHIGVNSLPLVLIIAVFTGAVSAWQAAYQLKGIAPLSFLGGATSRAIITELGPV
                     LTGIVIAGRVGASIAAELGTMKVTEQIDALETMAINPVRYLAMPRFLAAIIMMPVLVI
                     FANTIAVFGAYLVSNYFLGVSFAVFFNSVSRFFDFGDLISGLIKTIFFGGVTSLLGCH
                     IGFRTEGGAEGVGLATIRSFVISAALILILDYVLWMLIF"
     gene            2578..3666
                     /locus_tag="VF870_00170"
     CDS             2578..3666
                     /locus_tag="VF870_00170"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX9250619.1"
                     /translation="MEQIKYIIKERSEKVILPSKLGFGQIFTDHIFEMDYTKDKGWHN
                     PTIKPIENLQMHPAISVIHYGQSIFEGLKAFKTIDDEVVIFRPDLHMQRLNNSAKRIC
                     MPEVDVDFALKALKELVSIDSDWIPVNRGEALYIRPFMFGTDPALGVRPSFDYKLVFL
                     LSPVGAYYPEGFKPVKILVQDDYVRAVRKGLGECKTSANYAASLLAAQEASKKGFTQV
                     LWLDGVEQRYLEEVGTMNIFVQFKDEIATPKLSGSILPGVTRRSVIQILKEWGMNVTE
                     RAISIDEVISAYDKGNLVGLFGTGTAAVISSIGWLTYKDKNMTFNNGQPGELDLKLFN
                     ELTSIHRREKEDMHNWLFKVEKKAVEVE"
     gene            3666..>4226
                     /locus_tag="VF870_00175"
     CDS             3666..>4226
                     /locus_tag="VF870_00175"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX9250620.1"
                     /translation="MLIRSVVILFFVAFNFISAQNAKPKNIIILIGDGMGINYVGASL
                     LQDPNSPFKEFKSIGLSITCSADKLITDSAAGATAIATGYLTNNKYISVDTSGAPLRT
                     LFEQAEKLGLSTGIVVTASVAHATPGAFLGHSISRYDQTLIASQMVEQNFDVIIGGGL
                     KYFLPKSLSGERDDNKNLLDVLTSKNY"
BASE COUNT         1402 a          666 c          712 g         1446 t
ORIGIN      
        1 agaggaagat ttagggattt aatttcatca ccggtaaagc tttgcaggtc aaaaatatta
       61 cttttgaaat atgatggctc aattaaatca taaaaacttt ttgaaagtaa ttcctcttca
      121 gtatagccta atattttttg aaaagcaaaa ttagcgaata atatcaatcc atctttatca
      181 atattgcaaa tcattaatgg tgattttgaa attatttcgt gcaatccttt taatgatagc
      241 tttaatcctt cctcaaaatg taatttatct gttatatcaa cactcttaac aacaattctt
      301 tttttctttt cgtcaaggaa agtaaaataa gtctcataag tttttttatc tctgttctta
      361 tcaaagtatg agagaattat tttatgaatt tctttatcga ttagtttaga tttcaatgaa
      421 tcaagaaaat catccgagag aatgcttaca acattttgtg cagtaactcc aagtgcctcc
      481 gctttagaaa cctcgaaaag tttttcagcg caatcgtccc aatattcaat tatgttatca
      541 tcgttaatta caaaaacagc ttcgctggat ttatttgtta tggtttgata gagttcaaga
      601 gtaccaacct ctttataaag attatcaatt tctctctcct ggactgttat atccttgaaa
      661 aataaataaa caaaactgat ttctttccta aaattttctt gcggaacaaa acggcactca
      721 aatacttttc gtattcccct tatttcataa gaaagttttt cggtaagtac aatgcggttt
      781 ttcttagcat attcaaaata ctctttaaca acttttgaag tctcgagatc aaataaactt
      841 ccaagggaga cattttgaat taaattaacc gaaacattta tataatgtaa aatgttttta
      901 gaaaaaaact gtacacgttc cagtttatct acaaccagaa aaaaatcgga aacattgtgt
      961 atagcgtttt caataaaatc atttcttata gcagattctt tttttgtttt ggatagaatt
     1021 tttaaatctt caacaattat taatccgcca ataatttctt caccatcaaa aattggggag
     1081 ccttttataa ttaaatgaat taacccctgc gtttttgttt caacaaagtt tagttctttt
     1141 tcaaaaggaa gtccggataa aatatctttc agttcagtag taattgtgaa ttttggaaaa
     1201 agttctttcc cgaaaatact tgaacccatc aggttggctg gatattcaaa cccatataat
     1261 aattcgagat cggcaaaaga tttattggca taatcgattt cgccattctt attaaaagta
     1321 agaattgcaa ttggggctcc atcgataagg tttagagtat ttggttccaa gttttattaa
     1381 tcatttttta aaaaactaaa cagctaaaaa atacggtcat taagcggaac atatctattc
     1441 ccgttaaaat cgctgattgt gataataact ataatgccat agtattattg gtttaagtta
     1501 aaaattacca caattcagcg agaaaataac atctaaaaat ttgtttgatg tctcgttttg
     1561 aataaatgta ttgatataaa tattttctta tgaataccgc gtgaaaaatt tcgtgcaatt
     1621 ttatcattgc taaaaacaat ttttccttca tttttcttta agtttgtaca tcaataatta
     1681 gaaaatttgt tttgatatcc gcagcatttg aaaaacttgg aaatagaaca ttagctttcc
     1741 tgcaggaatt tgggcagatc tctaatctat tttttggcat aattaaaagc ttccctcaaa
     1801 ttcccaagag taaaaaactt atcctttttc agatggaaca tattggtgta aattcacttc
     1861 cgctagttct tattattgct gtttttactg gtgctgtttc cgcatggcaa gcagcttatc
     1921 aattaaaagg aattgctcct ttatcgtttt taggaggggc aacctcccgc gcaattataa
     1981 cagaacttgg tccggtttta acgggaatag taattgctgg cagagtcggt gcttcaattg
     2041 ccgcagagct tggcactatg aaggtaacag aacaaattga tgcacttgaa actatggcga
     2101 tcaatcccgt tagatattta gcaatgccca gatttttggc tgcaataata atgatgcctg
     2161 tgcttgtaat ctttgcaaac actattgcgg tttttggggc atatttagtg tctaattatt
     2221 ttcttggagt atcatttgct gtattcttta actctgtcag cagatttttt gattttgggg
     2281 atttaatttc gggattaatt aaaacaattt tttttggtgg agtaacgtcc cttttaggat
     2341 gtcatatagg gtttagaaca gaaggcggag cagaaggagt cggtttggca actataagat
     2401 catttgtaat ttctgcagca ttaattttaa tattggatta tgtcctctgg atgctgatct
     2461 tttaaatgct aaaaaaataa taaaaatttt aaattgcttc ttaattaata acatatatat
     2521 tataattcaa ataatttaaa atcagaacta ccttcaataa aaaggaagga acgaataatg
     2581 gaacaaatta agtacatcat taaagaaaga tcagaaaaag ttatacttcc ctccaaatta
     2641 ggttttggac aaatatttac cgatcacatt tttgaaatgg attatacgaa agataaggga
     2701 tggcataacc caactataaa acctattgaa aatcttcaga tgcatcctgc aatatcagta
     2761 attcattatg gtcaatctat ttttgaaggc ctaaaggcat ttaaaacaat agacgatgaa
     2821 gttgtaattt tccgtcctga tcttcatatg caaaggctaa ataattctgc aaaacgaatt
     2881 tgcatgcctg aagttgatgt tgattttgct cttaaagcat tgaaagaact tgtttcaatt
     2941 gatagtgatt ggattccggt aaatagaggt gaggcactct acatcagacc gtttatgttt
     3001 ggtacagatc ccgcacttgg tgtaagacca tcatttgatt acaaattagt tttcttactt
     3061 tctccggttg gtgcttatta tccggaagga tttaaaccag ttaagatttt agtacaggat
     3121 gattatgtcc gtgcagtcag aaaaggttta ggtgaatgta aaacatctgc aaattatgcc
     3181 gcaagtttgt tagctgcaca ggaagcaagc aagaaaggat ttactcaggt tctttggctt
     3241 gatggtgttg agcaaagata tttagaagaa gtcgggacaa tgaatatttt tgtccaattt
     3301 aaagatgaaa ttgctactcc aaaattaagc ggatcaatcc tgccgggagt tacaagacga
     3361 tcagtaattc aaattttaaa agagtgggga atgaacgtta ccgaaagagc aatctcaatt
     3421 gatgaagtta tatcagctta tgataaagga aaccttgttg gattatttgg caccggcaca
     3481 gcagcagtta tatcttctat tggctggctt acatataaag ataaaaatat gacttttaat
     3541 aacggacagc ccggtgaact tgacctgaaa ttatttaatg aacttacatc gattcaccgc
     3601 agagaaaaag aagacatgca caattggtta tttaaagtag aaaaaaaggc tgttgaagta
     3661 gaataatgct gatacgttct gttgttatac ttttttttgt agcatttaat tttataagtg
     3721 cacagaatgc aaaacctaaa aatattatta tcctaatcgg agatggaatg ggcatcaact
     3781 atgttggtgc ctctttattg caggatccga actcaccatt taaagaattt aaatcaattg
     3841 gactctcaat aacctgttct gcagataaat taattactga ttctgcagcg ggtgcaactg
     3901 ccattgcaac aggatatctt acaaacaata aatacatttc tgttgataca tcgggtgctc
     3961 ctcttcgcac attatttgaa caagcagaaa agttaggttt atctactggt atagttgtaa
     4021 ctgcttctgt ggcgcacgct acacctggag cttttctagg gcattcaatc agcagatatg
     4081 atcaaactct tattgcctct caaatggtag aacaaaattt tgatgtgata atcggcggcg
     4141 gattaaaata ttttttgccc aaaagtttat ccggtgaaag agatgataac aaaaatcttt
     4201 tagatgtttt aacttctaaa aattac
//
LOCUS       DASWBY010000008         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_564362, whole genome shotgun sequence.
ACCESSION   DASWBY010000008 DASWBY010000000
VERSION     DASWBY010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564362"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VF870_00180"
     CDS             <1..858
                     /locus_tag="VF870_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9250621.1"
                     /translation="ASTDVSVLIEGETGTGKELIARAIHKNSSRKDKPFIKVNCASIP
                     ENLIESELFGHERGAFTGATGKRIGRFEIANGGTIFLDEIGELPINLQPKLLTVLQQG
                     EFERIGSSKTIKTDVRVIAATNKTLTDEIKEGNFRSDLYYRLNVFPITVAPLRERKED
                     IPPLIEHYVNLYSKKLNKPIEIIPKSAMQRLIDYSWPGNVRELENIIERAIIISTSKT
                     LNVESLPKTENKSSKQLTLEENEINHIVETLEKTDWKISGPNSASELLKINPNTLHSR
                     MRKLRIKRK"
     gene            1046..1321
                     /locus_tag="VF870_00185"
     CDS             1046..1321
                     /locus_tag="VF870_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250622.1"
                     /translation="METITISASISEKKKVEFFQTMESLTRFVKSQCKDLEIRVKDDN
                     SLIIKIIFDGKDQMERNFYSKEFTIIKGSVKSLCKDVIIKVNGSSEL"
     gene            1403..>3008
                     /locus_tag="VF870_00190"
     CDS             1403..>3008
                     /locus_tag="VF870_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX9250623.1"
                     /translation="MIDELNTVKLPDVSKFHCFKSARKKGPDSLSWLLRSINLISLLI
                     GLVAVTAISDGFAQDSQRRPNIVIILGDDLGYSDMGSFGSEIQTPNLDKLANEGVRFN
                     NFYTHASCSPTRSMLLSGVDTHLNGLGNMNEWTAPNQTGVPGYEGHLSSQVTTLPLLL
                     KQAGYHTYMVGKWHMGKSPELIPSARGFERDFSLLDGAGSYWDMTNFTATCPKSVYTE
                     DGKYLTELPDDYYATKTYTDKLIGYIEDNRKDGKPFFAYVSHQAPHDPYHLPKDWRNR
                     HVGEYDKGWDQVRQDRLKKQIELGIMPAGTELAERMWFLPDPLTLAPASKAILGKKME
                     LYAGMVENMDYHIGRLIDYLKEIGEYENTIFIVFGDNGAEGTDVFKMIAGTPGTRDFL
                     FAAKNWSETDPNAWGDPGTYVAYGPMWAQVSMTPFSQYKGWLGEGGIHNALIVSGPVV
                     KRPAGSINNGQMHVADIMPTLLEIANAKYPKTLENGHELAPLIGKSWLPMLSGKTESP
                     RTEKDYLAWEIFGNRALVQGDWKIRWE"
BASE COUNT         1044 a          518 c          605 g          841 t
ORIGIN      
        1 gcatcgaccg acgtttccgt acttattgaa ggtgaaacgg gcactggtaa agagttaatt
       61 gcaagagcta tccataaaaa tagtagtaga aaagataaac catttattaa agttaattgt
      121 gcttcaattc ctgaaaacct aattgaaagc gaattgttcg gacacgaaag aggggctttt
      181 actggcgcaa cagggaaacg aatcgggaga tttgaaatag caaacggagg aacaatattc
      241 cttgatgaaa ttggagagct gccaatcaat cttcaaccaa aacttcttac ggtattgcag
      301 cagggtgaat tcgaaagaat tggaagttcc aaaacaatta aaactgatgt tagagtaatt
      361 gctgccacaa ataaaactct tacagatgaa attaaagaag gaaattttag aagcgatctg
      421 tattacagat taaacgtatt tccaattact gtagcacctc ttagagaaag aaaagaagat
      481 atacctccat tgattgagca ctatgttaat ttgtattcta aaaaacttaa caagccaata
      541 gagattattc ctaaatcagc tatgcagcga ttaattgatt attcctggcc aggcaatgtt
      601 agggaacttg aaaacataat tgaaagagcg ataattatat cgaccagcaa aactttaaat
      661 gtagaatctc ttcccaaaac tgaaaacaaa tcaagtaaac aacttacact tgaagagaat
      721 gaaataaatc atatagtaga gacacttgaa aaaacagact ggaaaatcag cggccccaac
      781 agtgcttcag agttacttaa aatcaatccg aatacacttc attcacgtat gcgaaaactc
      841 agaattaaac gtaaatagtt cataaaaata ttctgttata tttaacaaca ttgttttatt
      901 taacaagcat tcacttccct gattcaatca aatactttca aaataatcac tattcaaata
      961 atttacattt ggctgattat tgggtaacaa tagtttaggt tagctaagat taaatcgatg
     1021 accatatcca gaaagcaaat tatttatgga gactataaca attagtgcaa gtatcagtga
     1081 gaagaaaaaa gtagaatttt ttcaaacaat ggaatcactt acaagatttg ttaaaagcca
     1141 atgcaaagat ttagaaataa gagttaagga tgataatagc ctgatcatca aaataatttt
     1201 cgacggaaaa gatcaaatgg aaagaaattt ttatagcaag gagtttacca taattaaagg
     1261 ttcagtgaaa agtctttgta aagacgtaat aattaaagtg aatggctcta gtgaattata
     1321 attagcaggc aaatttctga tttggtgatt ttgaaaaaaa taaaaaaagt taataaccct
     1381 caacaaacga aggagttcaa tcatgattga tgaattgaat acagtgaaac ttcctgatgt
     1441 ctctaagttt cattgtttta aatctgcaag aaaaaaagga ccggacagtt taagctggtt
     1501 actcagatca attaatctaa tttctcttct tataggactg gtcgcagtta ccgcaatatc
     1561 tgacggcttc gcacaagatt cgcagcgcag accaaacata gtaataattc ttggagatga
     1621 tttgggatat tctgatatgg gttcttttgg cagtgaaatt caaaccccaa atttggataa
     1681 actggcaaat gaaggagtac gctttaataa tttctatact cacgccagtt gttcccctac
     1741 aagatctatg ttgttaagcg gtgtagatac acatttaaac ggtcttggca atatgaatga
     1801 atggaccgca ccaaatcaaa ctggtgttcc aggttatgaa ggtcatctca gctctcaggt
     1861 tacgaccctt ccgttattgc tgaagcaagc cggttatcac acttatatgg tcggcaaatg
     1921 gcatatgggt aaatcaccag agcttatacc ctcagccaga ggatttgaaa gagacttctc
     1981 acttcttgac ggagccggga gttattggga tatgacaaac ttcaccgcta catgtccaaa
     2041 atctgtttat accgaagatg gtaaatatct tacagaactt cctgatgatt actacgctac
     2101 taaaacttac actgataagt taattggata tatagaggat aaccgaaaag atggtaaacc
     2161 atttttcgcg tatgtttctc atcaggcacc gcacgaccca tatcatcttc cgaaagattg
     2221 gcgcaatcgg catgtgggtg aatacgataa aggttgggat caggttcgtc aggatcgtct
     2281 gaaaaagcaa attgagcttg gtattatgcc tgctggtaca gaactggctg aaagaatgtg
     2341 gtttctgccc gatccactta ctttagctcc agcgtcaaag gcaattctcg gtaagaagat
     2401 ggaactctac gccggtatgg ttgagaatat ggattatcac attggcagat taatcgatta
     2461 ccttaaagaa attggagagt atgaaaatac tatattcatc gttttcggtg ataatggtgc
     2521 cgaaggcact gacgtcttta aaatgatcgc aggtacacca ggcacacgtg attttttgtt
     2581 tgcggcaaag aactggtccg aaaccgatcc gaatgcttgg ggtgatccgg gaacttatgt
     2641 tgcttacggt ccgatgtggg cacaggtatc aatgacgcca ttcagtcagt ataaaggctg
     2701 gctaggtgaa ggtggtatac ataacgctct tattgtaagt ggtcctgttg taaaaagacc
     2761 cgccggaagt attaataatg gtcagatgca cgttgctgat attatgccga ccttacttga
     2821 aattgctaat gccaaatatc ctaagacatt agaaaatgga catgaactag cccctcttat
     2881 tggtaaatcg tggctgccga tgttatcagg taaaacagaa tcaccaagaa ctgaaaagga
     2941 ctaccttgct tgggaaatat tcggtaaccg tgcattggtg caaggagatt ggaaaattcg
     3001 ctgggaat
//
LOCUS       DASWBY010000009         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_526758, whole genome shotgun sequence.
ACCESSION   DASWBY010000009 DASWBY010000000
VERSION     DASWBY010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526758"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VF870_00195"
     CDS             complement(<1..441)
                     /locus_tag="VF870_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HEX9250624.1"
                     /translation="MALRLSSNEKLRCFPNILKKIQTLLVIGLVILPTNIYAQSNDDC
                     LMCHSDNELTLEKKGKEISLFVDENVFKGSTHSKLSCVSCHKGFDPESMPHAEKIQEI
                     NCVSCHQKVLTQHSFHPQILKSNGLGKTTDTNCKSCHGIHDVVSP"
     gene            complement(469..804)
                     /locus_tag="VF870_00200"
     CDS             complement(469..804)
                     /locus_tag="VF870_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9250625.1"
                     /translation="MKNILIYLGLVITIVALYGFAFTIANQPGDDGKTIFVNNKCNMC
                     HTVTSAGIESKKSDAVDLSNVGKDKTVEFLTKFLKKEAKLNDKDHKASFKGTDEELTK
                     LVEWLSTLK"
     gene            complement(1100..1459)
                     /locus_tag="VF870_00205"
     CDS             complement(1100..1459)
                     /locus_tag="VF870_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250626.1"
                     /translation="MKNIVIYSPDFSLCYSLLMYLQTQYKVVATTDMEVVSSLICSGS
                     ADLVIMDTEPNLELLTKCEQFKKCKSEVPLILTYVFNNRIKESEGKIKKFVNEIFYKP
                     FDLNEISSKIPSLLPSI"
     gene            complement(1678..>2045)
                     /locus_tag="VF870_00210"
     CDS             complement(1678..>2045)
                     /locus_tag="VF870_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9250627.1"
                     /translation="KYRKELNKNIKGIDNEAMRALLSHEWKGEVRELENIIERAVIFC
                     KGDMITVQELPASFKPKMDDSDFSFNGSLDDSVRKFERDFILRALENNDNNKEKTADA
                     LRVGLSTLYRKLKELDIKI"
BASE COUNT          636 a          328 c          312 g          769 t
ORIGIN      
        1 aggagaaaca acatcgtgaa tgccgtgaca acttttacaa ttagtatctg tagtttttcc
       61 taatccattc gattttaaaa tttgtggatg aaaagaatgt tgtgttaata ctttttgatg
      121 acaactgacg caattaattt cctgaatttt ttcagcatgc ggcatacttt caggatcaaa
      181 tcctttatga caggaaacgc agcttaattt agagtgagtt gaacctttaa aaacattttc
      241 atcaacaaac agggaaattt ctttcccctt tttttccaaa gtgagttcat tatcactatg
      301 gcacatcaaa caatcatcat tcgattgagc atatatattt gtgggtagaa taactaatcc
      361 gattacaagg agggtttgta ttttttttaa gatgtttggg aagcatctta atttttcgtt
      421 tgatgataat ctaagtgcca tagatgataa acttttgcct ttatattcct atttaagagt
      481 tgataaccat tcaaccaatt ttgtaagttc ttcgtcagtc cctttaaatg atgctttatg
      541 atctttgtca tttaacttag cttctttttt caagaacttc gttaagaact caacagtttt
      601 atcttttcca acattagata aatctactgc atcagatttt ttagattcga ttccggcaga
      661 tgtaactgta tgacacatgt tacatttatt atttacaaat attgttttac cgtcatcgcc
      721 tggttgattt gcaatagtaa aagcaaaccc atataaagct acgattgtta taaccaatcc
      781 aagataaatc aatatatttt tcatgttttt atatcctttt aatttgaatt ttttagttat
      841 tgattttgtg cccttatgtt acaattagga taccactctt ttaatgtgaa ttatttgcta
      901 aaaatccaat attagctttg ttcagatttg tcaaatttct cagctttggg aagtcttttt
      961 agaacatatt cgcaagaatt ggaatgattt ctaaaattat tgcgcaggta gactaattga
     1021 atttgatgta taaaaaacta tactaactaa atgatttaat gaacacagaa ggatgttata
     1081 aaaatactaa tttaatttat taaatactgg gcagcaggga agggattttt gaagaaattt
     1141 cgttaagatc aaatggcttg taaaaaattt catttacaaa cttcttgatc tttccttcgg
     1201 actctttaat tctattgtta aagacataag ttagaattag aggtacttct gatttacatt
     1261 ttttaaattg ttcgcatttg gtaagaagtt ctaaattcgg ttccgtatcc ataataacta
     1321 aatctgcaga accgctgcat attagtgatg acacaacttc catatcggtt gtagcaacaa
     1381 ctttatattg tgtttgcaag tacatcagca agctgtaaca caaactgaaa tcggggctat
     1441 atataactat atttttcatt tactttattg tgattctttt cccctgaatg gagaagcagg
     1501 cataaatttg ctatttgtgt gccaaaattg attgtggagc tattgagcgg gtaatggttg
     1561 gtttttggaa ttattgcgca aaaaagcgta ttgttctatt ctcgcaattg aaaatcaata
     1621 aagctgtatt ttaattattg agaatatatc caaataaggg cagtgtgctt tactgtatta
     1681 aattttaata tccagctctt ttaattttcg atataaagta gataagccca cccttaaagc
     1741 atcggcagtt ttttctttat tgttatcgtt attctcgagc gcccttaaaa taaaatcgcg
     1801 ttcaaacttt cttacagaat catctagcga accattaaaa gagaaatctg aatcatccat
     1861 tttaggttta aatgatgccg gaagttcctg cactgtaatc atatctccct tgcaaaaaat
     1921 tacggcacgt tcaataatat tttctaattc tcttacttcg cctttccatt cgtgactcaa
     1981 taaagctctc atagcttcat tatcaatacc cttgatgttc ttgtttaatt cttttctgta
     2041 cttgt
//
LOCUS       DASWBY010000010         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_564624, whole genome shotgun sequence.
ACCESSION   DASWBY010000010 DASWBY010000000
VERSION     DASWBY010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564624"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VF870_00215"
     CDS             <1..328
                     /locus_tag="VF870_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX9250628.1"
                     /translation="SIVIPSLFLIYPFIFFKSKLSQTQSDFTKFVLGFPKVFSGSASL
                     VGPGLSQTSRDNFLGKTGLTGYWYIENGNPEELEKLNFYYAKNQSIWLDLEILAKSIN
                     KMWSKK"
     gene            331..1281
                     /locus_tag="VF870_00220"
     CDS             331..1281
                     /locus_tag="VF870_00220"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX9250629.1"
                     /translation="MAKTILDFEKPVYELESKLDEMKKFSDNLDIEKDVLRLEEKVRQ
                     LKEELYRDLSRWQRVQLARHPDRPYTLDYIYAMTESFVELHGDRSFKDDKAVVGGLAQ
                     IGQHKVVILGQQKGRDTKSNVYRNFGMMNPEGYRKALRLFKLAEKFNRPVITLIDTPG
                     AFPGLEAEERGQAEAIARNLFEMSKLKVPIIVVIIGEGASGGALGIGVGDRILMLENC
                     WYSVISPESCSSILWRSWEYKEQAAEALKLTAPDLLEQGIIDRIIKEPFGGAHKNHKE
                     AADNLKAVLIEELDGLIKIKPDKLVDNRIEKFGKMGVYTE"
     gene            1369..1779
                     /locus_tag="VF870_00225"
     CDS             1369..1779
                     /locus_tag="VF870_00225"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9250630.1"
                     /translation="MLKHYTELRVRYADTDQMRFAYNGKYFEYFEVGRTEMMREVGLP
                     YEVIEKSGFFMPVIETKIHFFSPAFYDELLEIETRVEKLPTSKVHIDHIIKAKERDVV
                     ICEGYVELAFLDAKSNRPTRAPKIFIDAVKKYYE"
     gene            1772..2620
                     /gene="nadC"
                     /locus_tag="VF870_00230"
     CDS             1772..2620
                     /gene="nadC"
                     /locus_tag="VF870_00230"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX9250631.1"
                     /translation="MSKIYKEVIKLINNALREDVKNGDITTKATILKSKKAVGKFLIK
                     ANGVIAGLEIAKAVFKQVDPKIKFEINIKDGSIVKYGDVAAIVSGKAQSLLTAERTAL
                     NFLQRMSGIATSANLFAEKVNHTKAKIIDTRKTVPGFRTLDKLAVKLGGCANHRIGLY
                     DMFLIKDNHIEVAGSITKAVEACVKYNKKHLTKFKIEVETKNLKEVEEALATKADVIM
                     LDNFEIEDMREAVKLINGKCKVEASGGVNLKTVKQIAETGVDFISVGALTHSVKALDI
                     SLEITI"
BASE COUNT         1003 a          381 c          563 g          839 t
ORIGIN      
        1 ctcaatagtt attccttctt tgtttttgat ttatcctttc atatttttca aaagcaaact
       61 ctcgcaaacg cagtccgatt tcacaaaatt tgttttaggg tttccaaaag tatttagcgg
      121 aagcgcaagt ttagttggtc ctggtttatc tcaaacatcc agggataatt ttttaggtaa
      181 aacaggttta accggatatt ggtatattga aaatggaaat ccggaagaac tggaaaagtt
      241 aaatttttat tacgcaaaaa atcaaagcat ttggctcgat ttagaaatac ttgctaaatc
      301 gataaataaa atgtggagca aaaagtaaag atggcaaaaa caattttaga ttttgaaaaa
      361 ccagtttatg agttagaatc aaaattggat gaaatgaaaa aattctccga taaccttgat
      421 atagaaaaag atgtattaag gctcgaggag aaagtaagac aattgaaaga agaattatat
      481 agagatcttt caagatggca gcgtgttcaa ttggcacgtc atccggatag accttatacg
      541 ctggattata tttatgcgat gactgaatct tttgtagagc ttcatggcga tagatctttc
      601 aaggatgata aagctgtcgt tggcggactt gcacaaattg gtcagcataa agttgtaatt
      661 ctgggtcagc agaaggggcg agacacaaag tccaacgttt atagaaattt tggaatgatg
      721 aatccagaag gttatcgtaa agcattacgt ttatttaaac ttgctgaaaa gtttaatcgg
      781 cctgttatta ctttaataga tactcccggc gcattcccgg gattagaagc tgaagaacgc
      841 gggcaggcag aagcaattgc cagaaatctt tttgagatga gcaaacttaa agttcctata
      901 attgttgtga taattggtga aggtgcaagc gggggcgcat tagggatcgg cgttggagat
      961 agaattttga tgctggaaaa ttgctggtat tcagttatca gtcctgagtc ttgctcttct
     1021 attttatgga gaagctggga atataaagaa caggctgctg aggcacttaa acttactgct
     1081 ccagatttat tagagcaggg gattatcgat agaataatta aagaaccttt tggcggtgct
     1141 cataaaaatc ataaagaggc ggcagataat ttaaaggcag tattgattga agaacttgat
     1201 ggactgatca aaataaaacc ggataaactt gttgataacc gtatagaaaa atttggtaag
     1261 atgggcgttt atactgaata atatgagata aatcgatttt aaattttaaa atcccgaata
     1321 gctcgggatt tttttatttt aactcatcaa aaataaaatt aaaattaaat gctaaaacat
     1381 tacacagaac ttagagttcg ttatgcagat acggatcaaa tgagatttgc atataatgga
     1441 aaatattttg agtactttga agtcggaaga acagaaatga tgcgcgaagt tggattgcct
     1501 tatgaagtaa tagaaaaaag cggattcttt atgccggtaa ttgaaactaa aattcatttt
     1561 ttttctcctg ctttttatga tgaactgttg gaaattgaaa ctcgcgttga aaaattaccg
     1621 acaagcaaag ttcatattga tcatataata aaagctaaag aaagagatgt tgtaatttgc
     1681 gaaggttatg ttgagttagc attccttgat gctaaatcaa atcgtcctac ccgggcacct
     1741 aaaatattta ttgatgcggt gaaaaaatat tatgagtaaa atctataaag aagtaataaa
     1801 gcttattaat aatgctttgc gggaagatgt aaagaatgga gatatcacaa ctaaagctac
     1861 aatattaaaa tcaaaaaaag cagtcggtaa atttctaata aaagcaaatg gagttattgc
     1921 cggattagaa attgcaaaag cagtatttaa acaagttgat cctaaaataa agtttgaaat
     1981 taatattaaa gacggttcta tagtaaaata tggtgatgtc gccgcaattg tttctggaaa
     2041 agctcaatca ctacttacag ctgaacgaac agcattgaat tttttacagc ggatgagcgg
     2101 tatcgcgaca tcagcaaatt tatttgcaga aaaagttaat cacacaaaag caaaaattat
     2161 tgataccaga aaaactgttc ccggttttcg cacgcttgat aaacttgctg ttaaacttgg
     2221 aggatgtgca aatcatcgta taggtttgta tgatatgttt ttaataaaag ataatcatat
     2281 tgaagttgcc ggctcaataa ctaaagcagt cgaagcttgt gtaaagtata ataaaaaaca
     2341 tctcaccaaa tttaagattg aagtggaaac aaaaaatcta aaagaagttg aagaagcttt
     2401 agcaacgaaa gcagatgtaa ttatgttaga taattttgaa atcgaagata tgagagaagc
     2461 cgtaaaactt attaatggta agtgtaaagt tgaagcatct ggcggagtga atttaaaaac
     2521 agtaaagcaa attgccgaaa cgggtgttga ttttatttct gtcggtgcat taacacacag
     2581 cgtaaaagct ctggatatct cacttgagat aacaatctag attagaatga aagaaaaatt
     2641 aaaagagtta acaaaggata cggctattta cggcatcagc acgatgctgg gcaggttttt
     2701 aaattttctt cttgttccat tttttacaaa tgtatttttg ccctcagagt atggtgttgt
     2761 taacatacta tattcttata tcgcaa
//
LOCUS       DASWBY010000011         3743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_565, whole genome shotgun sequence.
ACCESSION   DASWBY010000011 DASWBY010000000
VERSION     DASWBY010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF870_00235"
     CDS             complement(<1..387)
                     /locus_tag="VF870_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9250632.1"
                     /translation="MFKKILIANRGEIAVRIIKACRELGITSAAIYSDADLTSLHTRM
                     ADEAYHIGASAASESYLNKNKIIDLAKSIGADAIHPGYGFFSENASFIEEVEKSGITF
                     IGPSSKSVKMMGSKTAARTLMSKNNVP"
     gene            complement(396..2741)
                     /locus_tag="VF870_00240"
     CDS             complement(396..2741)
                     /locus_tag="VF870_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX9250633.1"
                     /translation="MIDERILEKLEFEKILNQVSKYSSTENGKNLILKLYPSSDLKFI
                     MTEGLLVEEAKEILNKAGHPPIDFLSDLNNSLSESRIEGAILSSNKVLEILKLAKTSR
                     LLIQFLKSELKDDSKLKEDLGSLFSDKLFEHHIEKVINEQGEVKENASTVLAQIRRDI
                     NSRRNELVKSINRIIKTLKDDDIVREDYLTLRDGRMVIPIKAEHKRHIRGFIHSESST
                     GQTVYIEPEETLDLNNDIISLSFAERREIERILRELTKMIGQSSELLKTSLEAIAYVD
                     SIFARAKYSIEIIGAFPQVDNSKPIFISEARHPILLKKLSRQKTVPLSLELKDDKVVI
                     ITGPNAGGKTVVLKTIGILSLMLQSGIHIPANSNSNFHIFENILIDIGDQQSIEDDLS
                     TFSSHLKNLKSIIENAGEDSLILLDEIGTGTDPAEGSALAAAILSTLLEKGSTVFAST
                     HHGSLKVFAYNIDGMQNAAMEFDHTNLSPTYKFNLGVPGSSYAFEIAKRTGLDDEVLE
                     KAKQNIDSDKHNLEKFLSELEEKSNKLNKKLNALEIENSRLEGLSALYKKSYEKIEKE
                     KKEILKKVKYDGELYLENVNKKIERVIKDLRESNASKDVIKESKKIIQEIKSENKELF
                     IEKIEKTSDKKNFAIDDFVGINNSSTTGKIIELNQNKGKATILSGSIKMQVNISDLFE
                     TKEKKIPVDNSFSSYKISDVNYRLDIRGEKPEVAEFQIIKFLDDAYQSSLERVEILHG
                     KGTGVLKKTVWEILKQHEKVKNFYFAAIEFGGEGITIAELK"
     gene            complement(2738..3259)
                     /locus_tag="VF870_00245"
     CDS             complement(2738..3259)
                     /locus_tag="VF870_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX9250634.1"
                     /translation="MNFIDIIIVLAIAIGFLLGFKDGFVRKLVGIIGFIAAVIIVILF
                     SSSVGKIIESFFGIEFYLAEIMGGLLLFVAIMIVTTILKRVIHPFDKVNNLINQLVGG
                     FVGILQLLFFLSAIFLLLNIFDFPDKKTQNSSVLYKYAYGFIPTSIDFLKDYTPSTED
                     VIKDYINQKDSIQ"
     gene            complement(3262..>3743)
                     /locus_tag="VF870_00250"
     CDS             complement(3262..>3743)
                     /locus_tag="VF870_00250"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX9250635.1"
                     /translation="FACLFQFIEGMMNLKDKINQDLKDAMKSGDKIRLSVVRSIRALI
                     LEFEKSGAAKELNPEEELKMLTTAAKKRKDSIEQFRNAGRTELAETEEAELKILMNYL
                     PKQMNEEEIKNEITRLAAEIGAKTKADFPKLMPLVMRELKGKADGKLVKSIVESYLV"
BASE COUNT         1151 a          684 c          534 g         1374 t
ORIGIN      
        1 cggaacatta tttttactca tcaaagttct tgctgcagtt ttacttccca tcatcttaac
       61 agattttgaa gaagggccga tgaatgttat tccacttttt tctacttctt caataaatga
      121 agcattttca gaaaagaatc cataacccgg atgaattgca tctgcaccaa tggattttgc
      181 aagatcaata atcttatttt tatttagata agattcagaa gctgcagatg ctccaatatg
      241 ataagcttca tcagccattc tggtatgtaa cgaagtcaga tcagcatcag aataaattgc
      301 tgcagatgta attccaagtt ccctgcaggc tttaattatt ctaacagcaa tttcacctcg
      361 gttagcaata agaattttct tgaacaaata aactcctatt ttaattctgc aatagtaata
      421 ccttcaccgc caaattcaat agcagcaaag taaaagtttt tcactttttc atgttgtttt
      481 aaaatttccc agactgtttt tttaagaact cctgtcccct taccgtgaag aatctcgacc
      541 ctttctaagg aactctggta agcatcatct aaaaatttaa ttatttgaaa ctccgcaacc
      601 tcaggttttt caccacggat atctaacctg taatttacat cagaaatttt ataagaagaa
      661 aaactattat ccactggtat tttcttttct tttgtttcaa aaaggtctga aatattaacc
      721 tgcattttta ttgagcctga taatatggtt gcctttcctt tgttttgatt cagctcaatt
      781 atttttcctg ttgtggatga attatttatt ccaacaaaat catcgatagc aaaattcttt
      841 ttatcacttg tcttttcaat tttttcgata aaaagctctt tgttttcaga ttttatttcc
      901 tggataattt tttttgattc ctttattaca tctttgcttg cattggattc acgtaaatct
      961 tttatcaccc tttcaatttt tttattcaca ttttcaagat aaagttcacc atcatactta
     1021 acttttttaa gaatttcttt tttctctttt tctatttttt cataactctt tttataaagt
     1081 gcagataagc cttccagtct tgaattttca atctcaagtg cattcaactt tttattcagc
     1141 ttgttggatt tttcctcaag ttcggataag aatttttcaa gattgtgttt atccgaatcg
     1201 atattttgtt tagccttttc caaaacctca tcatccaaac ctgtgcgctt tgcaatttca
     1261 aacgcataac ttgatcccgg tactccgaga ttaaacttat acgttggtga aagatttgtg
     1321 tgatcaaact ccatagctgc attctgcatt ccatcaatat tatatgcaaa tacttttaaa
     1381 ctaccgtgat gagtggatgc aaaaacagtt gatccctttt ctaataaggt tgataaaatt
     1441 gctgcggcca gagcagaacc ttctgcagga tcagttccgg ttccaatttc atccaatagt
     1501 atcagtgaat cttcgccggc attttcaatg atacttttaa gatttttcag atgagaacta
     1561 aatgtggata aatcatcttc aatagattgc tgatccccaa tgtcaattaa aatgttttcg
     1621 aatatgtgaa aattagagtt tgaattggca gggatatgaa ttccagactg aagcatcaaa
     1681 cttaaaattc caatagtttt aagtacaact gttttaccgc cggcatttgg gcctgtaatt
     1741 ataacaacct tatcatcctt aagttcaagg ctaaggggaa ccgtcttttg tctgcttagt
     1801 ttctttaaaa gaattggatg tctggcttca gaaataaata ttggttttga attatccact
     1861 tgcgggaatg caccgattat ctctatggaa tattttgctc gcgcaaagat agaatcaaca
     1921 taagcaatcg cttcaagtga agttttaagc agttcactcg attgacctat catctttgta
     1981 agctctctta agattctttc aatctcacgt ctctcagcaa aagaaagaga aataatatca
     2041 ttatttagat ccagcgtttc ttcaggttcg atataaacag tttgtcccgt tgaagattct
     2101 gaatgaataa atccgcgtat atgtctttta tgttcagctt taattggaat taccattcgc
     2161 ccgtcgcgca aggtcaaata atcttctctt acaatatcat catctttaag agttttaatg
     2221 atgcgattta tggattttac cagttcattt cttctggaat taatatccct tctaatctga
     2281 gcaagaacag tgcttgcatt ctctttaact tcgccttgtt cattaattac tttttcgata
     2341 tgatgttcaa ataatttatc agagaataaa gaacccaaat cttctttaag ttttgaatca
     2401 tcttttaatt ctgatttcag aaattgaatt agtaatcttg atgttttagc tagcttaaga
     2461 atttccaata ctttattact ggataatatt gctccttcta ttctgctctc agataaggaa
     2521 ttatttagat cagatagaaa atcaatcgga gggtgaccag ctttattcag tatctctttc
     2581 gcttcttcaa ctaaaagtcc ttcagtcatt ataaatttta gatctgaaga aggatatagt
     2641 tttaagataa gatttttgcc gttttcggta gaagaatatt tagaaacttg atttagaatt
     2701 ttttcaaact ctaatttttc taaaatccgc tcatcaatca ttggattgaa tctttttgat
     2761 ttatataatc tttaattaca tcctctgtgc ttggagtgta atccttaaga aaatcaattg
     2821 aagtgggaat aaaaccgtag gcatatttat aaagtacaga ggaattctga gttttcttat
     2881 ccgggaaatc aaaaatattc agtaacaaaa aaattgcact cagaaaaaat agcagttgta
     2941 gaattccaac aaatcccccg actaattggt tgataagatt attaacttta tcaaaaggat
     3001 gaattactct ttttaaaatt gtggtaacaa tcattatagc aacaaacagt aaaagcccac
     3061 ccataatttc agcgaggtaa aactcaattc caaaaaatga ttcaattatt tttccaacag
     3121 atgaagaaaa taatattaca attattactg ctgcaataaa tccaattatt ccaacaagtt
     3181 ttcttacaaa gccatcttta aatccaagta aaaaaccgat tgcaattgca agtactatta
     3241 taatatcgat gaaattcaaa atcagaccag gtaactttca acaattgatt taactaattt
     3301 tccatcggct ttacctttta attccctcat aaccagcggc ataagcttag gaaaatctgc
     3361 tttagtctta gccccgattt cagcagctaa tctagtaatc tcatttttaa tttcttcttc
     3421 gttcatttgt ttaggaagat aattcataag aatttttagt tctgcttctt ctgtttcagc
     3481 aagttcggtt ctacctgcat tacggaactg ctcaatagaa tcttttcttt tctttgctgc
     3541 agtagtaagc attttcaatt cttcttccgg attaagttct ttagcggcac cgcttttttc
     3601 gaattcaaga attaatgctc tgatagatct tacgacgcta agtctgattt tatcgccaga
     3661 cttcattgca tctttcaaat cctgatttat tttatctttt agattcatca taccctcgat
     3721 aaattgaaaa aggcaggcga ata
//
LOCUS       DASWBY010000012         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_564669, whole genome shotgun sequence.
ACCESSION   DASWBY010000012 DASWBY010000000
VERSION     DASWBY010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564669"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VF870_00255"
     CDS             <1..1446
                     /locus_tag="VF870_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250636.1"
                     /translation="ASVDVVSSQSNLQPTAGFNLEGEGLQLENVDSKETSLAGDIDAP
                     SISAMGAALKGVNLNKLEFIPALTEPAIYYHSYDGKKDLKGSKLLDEILSDIRQSKGV
                     NVEKESVDYIELADGSLLSAFLDGPVACVNLTDNLAGYFGKKFFKVPTIKSADTALAY
                     YVAKRKKFFPDDQSLIVYIGKEYSKLIFLQGRKLKHIGTTIDIGTTNLHTYDVYFSKI
                     LLEMENGGITSLDNIIVCGEDDSENLSLSFYGTFPEANVSRLEFDDVDMSSIDEETKA
                     KFSSYSVPLAVATEYFDELAKEHKGINLLPKYISEEQKLFQFSWHSFAVLPFLFLAAF
                     LFTLKVLQNTKELSRLDEEIKQKTLIIRQNQETLSKISEIEGKISSFGQTQAILDSAA
                     VGTGVWKSVLKDVAGFCGSKQNIWISSIVRDNTNTIKIEGYSLSKYSPTDMAYSLQNA
                     RLNSMMNEALREKNAYKYNLTFDITSYQNKK"
     gene            1443..>2036
                     /locus_tag="VF870_00260"
     CDS             1443..>2036
                     /locus_tag="VF870_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250637.1"
                     /translation="MNKKLRSTLVLLALLFVILIAGGAYLYFVQSKKLDEKRGKLQEL
                     QAKALDPQELLARYQELLVESNRLDSILANREFNIPQNLSSIKFYNFVNNITNGFSDK
                     AQVNIEFLDQKQEKEFFYHEYKLTGFGYFNEVYNLIFAIENSKELKKVTSVSLGNLVQ
                     TKADEVPVYLVNFTLNVKTYFSSDNRFAPATFVENNLG"
BASE COUNT          682 a          354 c          387 g          613 t
ORIGIN      
        1 gcatctgttg atgtggtctc atcacaatct aatttgcagc caacggcggg atttaatctg
       61 gaaggcgaag gacttcaact ggaaaatgtt gacagcaaag aaacttcact tgccggagat
      121 attgacgcgc caagcattag cgctatgggt gcggcattaa aaggagtaaa tcttaataaa
      181 ctggaattta ttcctgcatt aacagaaccc gcaatttatt atcactctta tgatggaaag
      241 aaggatttaa agggcagtaa actgctcgat gaaattcttt ctgatatccg gcaatctaag
      301 ggtgtaaatg tggaaaaaga aagcgtggat tatattgagc ttgcagatgg ctctttatta
      361 tctgcttttc tggatggacc ggttgcctgc gttaatctaa cggacaattt agccggatac
      421 tttggtaaaa agtttttcaa ggttcccacg atcaaaagtg cggataccgc tttggcatat
      481 tatgttgcca aacgaaaaaa attctttccc gatgatcaga gtttaatcgt ctacattggt
      541 aaagaataca gcaaacttat ctttctgcaa ggcagaaaac ttaagcatat tggaaccact
      601 atagatatcg ggacaaccaa tcttcacaca tacgatgttt atttctccaa gattctgctt
      661 gaaatggaaa acggaggaat aacttctctg gataatatta ttgtctgcgg tgaagatgac
      721 tctgaaaacc tgagtctttc tttttacgga acattccccg aagctaatgt aagcagatta
      781 gaatttgatg atgttgatat gtcatcaatt gatgaagaaa caaaagcaaa attttcttca
      841 tacagcgttc ctcttgctgt tgctaccgag tactttgatg aattggcaaa agagcacaaa
      901 ggtataaatc tattaccgaa atatatttca gaagaacaaa aattatttca gttctcctgg
      961 catagttttg cagttcttcc tttcttattt cttgctgctt ttctttttac gcttaaagta
     1021 ctgcaaaaca ctaaagaact ttccagactc gatgaagaga taaaacaaaa gactttaata
     1081 atcagacaga atcaggaaac acttagtaaa atttcagaga tcgaaggcaa gatcagcagt
     1141 ttcggtcaaa cacaggcaat tctggattcc gccgctgttg gtaccggtgt ttggaaatct
     1201 gttctaaaag atgttgccgg cttttgcgga agtaagcaga acatctggat atccagtatc
     1261 gttagagata acaccaatac aatcaagatt gaaggatact cgctttcaaa atactcccct
     1321 actgatatgg cttacagttt acaaaatgca agacttaaca gtatgatgaa tgaagctctt
     1381 agagaaaaga acgcttataa atataattta acatttgata ttacttcata tcagaataaa
     1441 aaatgaataa aaaattacga agcactttag ttttattggc tctcctattt gtaatcctca
     1501 ttgcaggagg agcatatctc tatttcgttc aaagtaaaaa attggatgag aagagaggaa
     1561 agctgcagga actacaggca aaagcattag acccgcaaga attgctggcg cgatatcagg
     1621 aacttttagt tgaatctaat cgtcttgatt caattcttgc caaccgggaa tttaatatac
     1681 cgcagaattt atcatccatt aagttttaca actttgtaaa taatattact aatggatttt
     1741 ctgataaagc ccaggttaat attgaatttc tagatcaaaa acaggaaaaa gaatttttct
     1801 atcacgagta taagctcacg ggcttcggtt actttaacga agtatacaat cttattttcg
     1861 caatagaaaa cagtaaggag ttaaaaaagg taacaagcgt tagtttaggc aatttggttc
     1921 agactaaagc tgatgaggtt ccggtttatc ttgttaactt tactctaaat gtaaaaacat
     1981 acttttcctc ggataaccgt tttgcaccag caacatttgt agaaaataat cttggt
//
LOCUS       DASWBY010000013         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_150985, whole genome shotgun sequence.
ACCESSION   DASWBY010000013 DASWBY010000000
VERSION     DASWBY010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150985"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VF870_00265"
     CDS             <1..697
                     /locus_tag="VF870_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HEX9250638.1"
                     /translation="GEVEASKTLEHFLKNKFENYHELRNDPTKEYQSNLSPYLHFGQI
                     SAQRIALDTERFNGNPESQKVFLEELIVRKELSDNFCYFNKKYDSFEGFHDWAKTSLN
                     EHRKDEREFVYTLEQFEQAKTHEDLWNAAQLEMIETGKMHGYMRMYWAKKILEWSKSP
                     EEALKISIYLNDKYELDGRDPNGYTGMAWSIGGIHDRPWFERPVYGKIRYMNRNGAEK
                     KFDIKSYIGKYLK"
     gene            897..>1912
                     /locus_tag="VF870_00270"
     CDS             897..>1912
                     /locus_tag="VF870_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9250639.1"
                     /translation="MKSRSQKLQLKLTIQFALFFIIVAGFIYFYFTDKFEGQVNDKYK
                     YKADVFVNFFTQNPQIFSDRKFNDPEIITKLLELNNGVYLVVENSKGELLDAINLEIA
                     EKSFYILAKSDDNRISKDEKVYRVELPIKTEETYGKIYVGFESGDDAKNIFKNRLLTA
                     LFSLCILLAGIVFTYFMSSISFRPLSKIIKALDSGLVNDGADKSKIYKKDELGIIEDR
                     VKTLMSELDKSTSEVEYLNKKLHDVFKDKISELNFEINQRKKAELSLQKSEEQFRLVF
                     QNAPIGIVIISTEGKIISVNKSFCDTIGFERDEIIGIPIKYLFEQNDLEGFSEGTLDN
                     NGNPV"
BASE COUNT          712 a          262 c          348 g          590 t
ORIGIN      
        1 cggagaagtt gaagcttcaa aaactttaga gcattttttg aagaacaaat ttgaaaatta
       61 tcacgaatta agaaacgatc ctacaaaaga atatcaatca aatctttcgc cttatttgca
      121 cttcggacaa atttctgctc agagaattgc actcgataca gagaggttta acggaaatcc
      181 agaatctcaa aaagtttttc tggaagaatt aattgtcaga aaagagcttt cagataattt
      241 ttgttatttc aataaaaaat atgattcctt cgaaggattt cacgactggg caaaaacatc
      301 tttaaatgag catagaaaag atgaacgtga atttgtttat acacttgagc aatttgagca
      361 ggcaaaaact catgaagatt tatggaatgc tgcgcagttg gaaatgattg aaacaggaaa
      421 aatgcatggc tatatgcgca tgtattgggc aaaaaaaatt cttgagtgga gcaaatcacc
      481 tgaagaagct ttaaaaatat caatctactt aaatgacaag tatgaacttg atggacgaga
      541 tccaaacgga tatacgggaa tggcgtggtc tataggcgga attcacgatc gtccgtggtt
      601 tgaaagaccg gtttacggca aaatcaggta catgaatcgc aatggtgctg agaaaaaatt
      661 tgatataaaa tcatacatcg gtaaatatct gaaatgacct ttattcgttc agattgtgta
      721 aaattatcgt aaaaccgtat tattttctca aattaaaaca tataaagctg cattatatta
      781 gaaaaacaga tgtttcttgt ttttagaaca acggcactta acttgatcgt aattgatcga
      841 ttaaataata gaggattaaa attctttgta tcaaaaaatg aggaaactta actgaaatga
      901 aatcccgctc acaaaaactt cagttaaagt taacaattca atttgccctt ttctttatta
      961 tcgttgccgg gtttatctac ttttatttta cggataaatt tgagggacag gtaaatgata
     1021 aatacaagta caaagctgac gtttttgtaa acttttttac acagaatcct cagatatttt
     1081 ctgatcgtaa attcaacgat ccagaaataa tcacaaaact tttagagtta aataatgggg
     1141 tttacctcgt tgtggaaaac tctaaaggag aactccttga tgcaataaat ttagaaattg
     1201 ctgaaaaaag tttttatatc cttgccaaat ctgatgataa tagaatttca aaagacgaga
     1261 aagtatatcg tgtggagttg ccgataaaaa cagaagaaac ttatgggaaa atatatgttg
     1321 gatttgaatc aggtgatgat gccaaaaata tttttaagaa tagattattg actgcacttt
     1381 tcagtctatg tattctgcta gcaggaattg tttttactta ctttatgagt tcaatttctt
     1441 tccgtccatt atctaaaatt attaaagcac ttgattccgg acttgttaac gatggagcgg
     1501 ataaatctaa aatttataaa aaagatgaac tcggaataat cgaagatcgt gtaaaaacac
     1561 ttatgtctga gttagataaa tctacttctg aagttgagta tttaaataaa aagttacacg
     1621 atgtatttaa ggataaaata tctgaactga attttgaaat caatcaaaga aaaaaagctg
     1681 agttatcact gcaaaaaagt gaggaacaat ttagactcgt atttcagaat gcacctatag
     1741 ggattgtgat tatatccaca gaaggaaaaa ttataagcgt taataaatca ttttgtgata
     1801 caataggatt tgaaagagat gaaattattg gaatacctat taagtatctg ttcgagcaga
     1861 atgatttaga aggctttagt gagggtacgc tggataacaa tggaaatcct gt
//
LOCUS       DASWBY010000014         4806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_339129, whole genome shotgun sequence.
ACCESSION   DASWBY010000014 DASWBY010000000
VERSION     DASWBY010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339129"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VF870_00275"
     CDS             complement(<1..1024)
                     /locus_tag="VF870_00275"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9250640.1"
                     /translation="MKVVLKNVNLLNPHQKLNEKSVSILIENGKFKKIGNVTEAEIKD
                     AKVFELDGKYAVPGLFDMHVHLREPGREDEETVVTGCNSAANGGFTGIACMPNTDPAI
                     DSAEVVTLIKKQSANHLVDVYPIGAATVGRKGEVLSPMAELKDAGVVGFSDDGIAIKT
                     ASILKRSLEYAKMFNLPIIEHCEDESLAGGAMNESVNSTLLGLPPIPSVAEDLIVMRD
                     ILMAEYTDAKIHIAHISSKKSVEMVREAKKKGIKVTAEVAPHHFTLTDDAVKTYDTNV
                     KMNPPLRTKADVDEIIKGLKDGTIDCIASDHAPHSLEEKEMEFEYAPNGIIGLETQVG
                     LAFNELY"
     gene            complement(1045..1965)
                     /locus_tag="VF870_00280"
     CDS             complement(1045..1965)
                     /locus_tag="VF870_00280"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX9250641.1"
                     /translation="MPLSSRHLLGLKDVPREDIQLILDTATTFREILERPIKKVPTLQ
                     GKTIVNLFYESSTRTRISFELAEKRLSADSINFAVSGSSVSKGETLKDTVKNIEAMKV
                     DMIVVRHACAGVPLMITKISDSNVINAGDGIHEHPTQGLLDMYSIREKLGRLEGLKVC
                     IVGDVAHSRVALSNIYGLKTMGAKVSVCGPSTMIPKFLDELGVDVIHNIDEAIQENDV
                     LNVLRIQLERKAREYFPSIREYSQYFGIDRERLEKNGKDILILHPGPINRGVELSSEI
                     ADGNSQIILQQVTNGVAVRMAVLYLLGTLN"
     gene            complement(1971..2516)
                     /gene="pyrR"
                     /locus_tag="VF870_00285"
     CDS             complement(1971..2516)
                     /gene="pyrR"
                     /locus_tag="VF870_00285"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX9250642.1"
                     /translation="MDVKSKIIDAEGFDRILTRIAHEILEKNKGSHNLVLMGMRTRGE
                     FLGKRIAEKIKQIESIDIPFGVLDVTLYRDDFRTRLKQPEVSVSNITFDINEKDIILV
                     DDVLFTGRTVRSALNAIMDMGRPSSIQLCILIDRGHRELPIRADYVGKNIPTSHNEEI
                     KVKIKEHDGEDAIYIVESQEK"
     gene            2729..2803
                     /locus_tag="VF870_00290"
     tRNA            2729..2803
                     /locus_tag="VF870_00290"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2764..2766,aa:Pro,seq:cgg)
     gene            2824..3321
                     /locus_tag="VF870_00295"
     CDS             2824..3321
                     /locus_tag="VF870_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX9250643.1"
                     /translation="MAQNHSFDIVSEIDFQEADNAVNQALKEIHQRYDLKDSNTQLEL
                     NKKDKLITMNTKDDYSRKSAIDILETKFIRRNISIKALKFNEPEAAAGGRLKQVINLQ
                     SGISKENAKIITKMIKESKLKVNTQIQDEQIRVTAPKIDDLQAVITLVKETELDFPTQ
                     FVNMK"
     gene            complement(3625..>4806)
                     /locus_tag="VF870_00300"
     CDS             complement(3625..>4806)
                     /locus_tag="VF870_00300"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX9250644.1"
                     /translation="SGKMRSHSAMQKIIEKYFSSCKNKGLSDTQAKELWRQIESFAGY
                     SFCKAHSASFALLSYQVAFLKSHYPAEFMASVLSNNGGFYSAAVYVWECKRLELKILL
                     PSINKSNYEYTGKKDEIQIGLMAIKNLSYNSCQKILDERKKDGDFYSLADFLIRTKLA
                     FEETSILIKCGAMGCLKKTRPELLRLLDIYIHKRKILNESYNDLFLHETFAMEDEVKT
                     NINFSVEEICKAEYETFDYMVTRHPLYFYRSFTEHYLIVKAKDLYKHRGKNVQLIGWY
                     MTSKRIKTRKGDIMKFLSLEDTTGTFEVVIFPREYEKFAEQTMSMGPYIIEGKVDEND
                     ATNVVVSNLSVLSVASVKVADQKDSVDNKYFGDVEKVSEEDFMLLEGIDKEKLRLAYA
                     S"
BASE COUNT         1443 a          953 c          723 g         1687 t
ORIGIN      
        1 gatacaattc attaaatgct aacccaacct gggtttctaa tccaataatt ccatttggag
       61 catattcaaa ttccatttct ttttcctcca aagaatgtgg cgcgtggtct gatgcaatac
      121 aatctattgt accgtctttc aatcctttaa taatttcatc aacatctgct tttgttctta
      181 atggcggatt catcttaaca tttgtgtcat aagttttaac ggcatcatcg gttaaagtga
      241 aatggtgtgg tgcaacttct gctgtaactt ttattccttt cttctttgct tcacgaacca
      301 tctccacaga ttttttactg ctgatatgtg ctatatgaat ttttgcatct gtatactcag
      361 ccattaaaat atcacgcatc actatcagat cttcggctac agatggaatt gggggaagac
      421 caagtaatgt tgagttaaca ctttcattca tagcgccacc ggcaagtgat tcatcctcac
      481 aatgctcaat tattggaaga ttaaacatct tagcgtattc taaagatctt tttagaattg
      541 aagcagtttt aattgcaatt ccgtcatcag aaaaaccaac cacgccggca tcttttaatt
      601 ccgccatcgg agaaagaact tcaccttttc ttccaacagt tgcggcacca atcggataaa
      661 catcaaccag atgatttgca gattgttttt taattaaagt tactacttcc gcggaatcaa
      721 ttgccggatc agtatttggc atacaggcaa ttccggtaaa tccgccgttt gcagcactgt
      781 tgcatcctgt aaccactgtt tcttcatctt ccctgcccgg ttctcttaag tgaacatgca
      841 tatcaaataa gccgggcaca gcatatttcc catccaattc aaatactttt gcatccttaa
      901 tttctgcttc agttacattt ccaatttttt tgaacttacc attttcaatc aaaatactta
      961 cacttttttc attaagcttt tgatgtggat ttagaagatt tacattcttt aaaactactt
     1021 tcattttcaa tactttctga ttttctaatt gagtgttcca agtaaataaa gcactgccat
     1081 tcttacagca accccatttg taacctgttg taaaattatt tgtgaatttc catcagcgat
     1141 ttccgaagat aattcaacac ccctgtttat agggcccgga tgaagtatca aaatatcttt
     1201 accatttttc tctaatcttt ccctgtcaat tccaaaatat tgcgagtact ctctaataga
     1261 agggaaatac tcccgggctt ttctttccaa ttgaattctt aaaacattaa gtacgtcatt
     1321 ttcctgtatt gcttcatcaa tattgtgaat tacatccacg ccaagttcat ccagaaattt
     1381 aggtatcatt gtagatggtc cgcaaacaga aacttttgca cccatcgtct tcaatccata
     1441 aatatttgag agcgcaacac gactgtgtgc aacatctcca acaatacaaa cttttaatcc
     1501 ttcaagtctg ccaagttttt cacggatcga atacatatca agcaaacctt gcgttgggtg
     1561 ttcatgaatt ccatcgccgg cattaataac atttgaatct gaaatttttg ttatcataag
     1621 tggtacaccc gcacaggcat gtcgaacgac aatcatatca actttcatcg cttcgatatt
     1681 ttttacagta tcctttaacg tttcaccttt gctaacactg cttccagata ctgcaaagtt
     1741 tattgaatca gccgataatc ttttttctgc cagctcaaaa gaaattcttg tacgggtaga
     1801 actttcataa aaaagattaa ctattgtctt accttgaagt gtaggaactt ttttaatcgg
     1861 tcgttcaagt atttcacgga aagttgttgc ggtgtctaaa attaattgga tatcctctct
     1921 gggaacatcc tttaatccta acaaatgtct gctagataat ggcatattta ttatttctct
     1981 tgtgattcaa caatgtagat ggcatcttca ccatcatgtt ctttaatttt tacttttatt
     2041 tcttcattgt gtgaagttgg aatatttttt ccaacataat ccgcacgaat tggcagctcg
     2101 cgatgtcccc ggtcaatcaa tatgcacaat tggatagaac ttggtcgccc catatccata
     2161 atggcattta gcgcagaacg aactgttctt ccggtaaaca aaacatcatc aaccagaata
     2221 atatctttct catttatgtc aaaggtgatg tttgaaactg aaacttcggg ctgttttaat
     2281 cttgttctaa aatcatcacg ataaagagtt acatcaagca ctccaaacgg aatatcgatg
     2341 ctttctatct gtttgatttt ttctgcaatt cgtttaccca aaaactcacc gcgtgttctc
     2401 attcccatta aaacaagatt gtgagaacct ttattttttt caagaatttc gtgagctatt
     2461 cttgtaagga ttcggtcaaa tccttctgca tcaattattt ttgattttac atccatatac
     2521 aaaaaagcct cttcggatta attatcctca gaggttacct tatttttagt ttacttttat
     2581 tttctttcat ccttttccat ctcacgggat agagttaaag gttaatttta tttgattcaa
     2641 acttaagggt attaattttt aaagtaaaga ataaaacacc tttgattaaa aatattttta
     2701 tatttgtttt gtagataaaa cactttttcg gggtgtagcg cagcccggct agcgcgcttc
     2761 gttcgggacg aagaggtcgt aggttcgaat cctatcaccc cgactaactt ttttaggtta
     2821 gtcatggcac aaaatcactc attcgatatt gtttctgaaa ttgattttca ggaagcagat
     2881 aatgctgtta atcaggcatt aaaagaaatt catcaacgtt atgatttgaa ggattctaac
     2941 actcagcttg agcttaacaa aaaagataag cttatcacca tgaatacaaa ggatgattac
     3001 tcccgtaaat ctgcaattga tattttggaa acaaaattta tccgtagaaa catttcgatt
     3061 aaggctctaa agtttaatga acctgaagct gctgcaggcg gaaggcttaa acaggttatt
     3121 aatcttcaaa gcggaatctc caaagaaaat gctaagatca ttacaaagat gattaaagaa
     3181 tccaagctta aggttaatac acagatacag gatgaacaaa ttagagtaac agctccaaag
     3241 attgatgatc tgcaagcagt tattacgttg gttaaagaaa ctgaattgga cttccctacc
     3301 cagtttgtga atatgaaata attagctgaa ataacattat gcaaataagt gtatataatt
     3361 tcattctttt atttgttttc ttgctttttg ttgtccactg gattgcaact cttattgttt
     3421 gtattcaaaa acaaaaatct aataaattat tttggtttct tatcatattt tttacctgtc
     3481 ccattggtca attctattat aggttcaaga ataatttacc aattcttggt ttttaaattt
     3541 ctataaattg atatctcgtt atttttatga atttgttttg attgcacctt cctcaacctt
     3601 aaggttaagg ctgcataatc aatatcaact cgcataagcc aatcttaact tttctttatc
     3661 aattccttcg agcagcataa aatcttcttc actaaccttt tcgacatcac caaaatattt
     3721 gttatcaacg ctgtctttct ggtctgcaac ttttacagat gcaacagata acacacttaa
     3781 attacttaca acaacatttg tagcatcgtt ctcatcaact ttgccttcaa taatatatgg
     3841 acccatagac attgtttgct cagcaaactt ctcatactca cgtggaaaga tcactacctc
     3901 aaaggttccc gttgtgtctt caagcgaaag aaacttcatt atatcacctt tgcgcgtttt
     3961 tattcttttc gatgtcatat accatccaat aagctgcaca ttttttccgc gatgtttata
     4021 taagtctttt gctttaacaa ttaaataatg ttcggtaaaa cttctataaa aataaagagg
     4081 gtgacgcgta accatataat caaaagtttc atattcagct ttacaaattt cttcaacgct
     4141 aaagttgatg tttgttttta cttcatcttc catcgcaaaa gtttcgtgta aaaaaagatc
     4201 gttataactt tcattaagaa tttttctttt gtggatgtaa atatcaagca aacgtaaaag
     4261 ttccggcctg gttttcttta agcatcccat agctccgcat ttgataagaa ttgatgtttc
     4321 ttcaaaggca agttttgtgc ggataagaaa atctgccagt gaataaaagt ctccatcttt
     4381 ttttctttca tccaaaattt tctggcagct attgtaagaa agatttttaa ttgccatcaa
     4441 tccaatttga atttcatctt tctttcccgt gtactcgtaa ttgcttttgt tgattgatgg
     4501 aagcagaatt tttaattcta atcttttgca ttcccacacg taaaccgcag cggaataaaa
     4561 tccaccatta ttgcttaaga cacttgccat aaactccgca ggataatgtg atttaagaaa
     4621 tgcaacctgg tacgaaagca aagcaaatga tgcactgtga gctttgcaga atgaatatcc
     4681 tgcaaaagat tctatctgtc tccataactc ttttgcttgt gtatcgctaa gccctttatt
     4741 cttacaagat gaaaaatatt tctcaattat tttttgcatt gccgaatgtg aacgcatctt
     4801 accgct
//
LOCUS       DASWBY010000015         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_639994, whole genome shotgun sequence.
ACCESSION   DASWBY010000015 DASWBY010000000
VERSION     DASWBY010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639994"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF870_00305"
     CDS             <1..460
                     /locus_tag="VF870_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9250645.1"
                     /translation="KIEKKFFEEQSELMATFIRILGIFITIIFSFGAIIGAMITMYSS
                     VANRTVEIGTLRALGFKRRSILSAFMAESLLVSIFGWIIGILLASALSFFKISTLNIG
                     SFSELEFSFALSPSIIILSLIFALLMGILGGFLPSVRAARLNIVKALRAG"
     gene            complement(531..1379)
                     /locus_tag="VF870_00310"
     CDS             complement(531..1379)
                     /locus_tag="VF870_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9250646.1"
                     /translation="MIKSKIDSKSIGIAFGAGGARGIAHLLMIEALDELGVKPSIISG
                     SSIGAVVGAFYAAGFSSKEMREILNQLINPKSDSVFDFLLKSDIVKMFTMFDPQFIKS
                     GFIKGEKFQRYMETHLKVSRFEELKIPLKIVATDYWKKEEVVFEKGELIQPIKASYSM
                     PGLFTPVNIKNRILIDGGTVNPLPFDLIKNDCDITIAIDVTAVKAQSNNDIPPTFDSV
                     FTTYQTMQNSIINERLKFLRPDIYIRPEIYDVRVFDFVKADSIFKQAQPAKEELKRQI
                     DILLNK"
     gene            complement(1438..>2053)
                     /locus_tag="VF870_00315"
     CDS             complement(1438..>2053)
                     /locus_tag="VF870_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEX9250647.1"
                     /translation="TKYLVEMLEEGLTDYILDGQTFDLEGVRSMRENANHVNTSPFTS
                     YNFHGKGNFASIVDTAVLGATEVDVKFNANVVTHSDGYLLHGIGGWQNCLYSKCTILA
                     IPSFRDRIPVIVDEVTTICGPGELIDVIITERGIAINPRRKDLLKAVKGSGLPIKDIK
                     QIKKEVDEICGGAPAKPKLIKDKVVAIVKWVDGTVLDSVFQVEK"
BASE COUNT          643 a          367 c          319 g          724 t
ORIGIN      
        1 aaaaattgaa aagaaatttt ttgaagagca atctgaattg atggcaacat ttattcgaat
       61 acttggaatc tttatcacaa ttatttttag ttttggcgct atcattgggg caatgataac
      121 tatgtattca tctgttgcaa acagaactgt tgagattgga acgttacgtg cacttggatt
      181 taagagaaga agcattcttt cggcatttat ggctgaatca cttttagtct cgatctttgg
      241 atggatcatc ggaatccttt tagcatcagc gttatcattc tttaaaatat caacattaaa
      301 tataggatcg ttttcagaat tggaattttc atttgctttg tctccatcta taataatttt
      361 atcattaata tttgcgctgt taatgggaat actaggcgga tttttacctt ctgttcgcgc
      421 tgctagatta aatattgtga aagctttaag ggctggataa gaattatgtt tgttattccc
      481 gcggaagcag gaaactactt aatgattaga gaatggaatt cattaatttt ttatttattc
      541 aaaagaatat ctatctgtct ttttaattct tctttagcgg gctgagcttg tttaaaaatt
      601 gaatcagctt taacaaaatc aaaaaccctt acatcataaa tttctggtcg aatataaata
      661 tctggtctta aaaattttaa tctctcattt ataatagaat tctgcattgt ctgataggta
      721 gtaaatacgg aatcaaatgt tggcggaata tcattattgc tttgtgcttt aacagcagta
      781 acatcaatag caatcgttat atcacaatca ttttttatta aatcaaatgg caagggattt
      841 acagtccctc cgtcaataag aattcgattt ttgatattta caggagtgaa aagtccaggc
      901 attgaatagc ttgcttttat tggctgaatt aattcacctt tttcaaaaac tacttcttcc
      961 tttttccaat aatctgtagc gacaattttt aatgggatct ttaattcttc aaatctggaa
     1021 acctttaaat gagtttccat atagcgctga aatttctccc ctttgatgaa accagatttg
     1081 atgaactgcg gatcgaacat tgtaaacatt tttacaatat cagatttaag taagaaatca
     1141 aaaacagaat cacttttagg gtttataagt tgattgagaa tttctctcat ctcttttgaa
     1201 gaaaatccag cagcataaaa tgccccaaca acagcaccaa tacttgaacc agaaataatc
     1261 gaaggtttaa caccaagttc atccagagct tcaatcatta ataaatgtgc aattccacgc
     1321 gcaccaccag caccaaatgc aatacctatg gattttgagt caatctttga ttttatcatt
     1381 taatgttccc gatcagtgta aatctgttca atcagtgtca tccgtgtccc atcgctctta
     1441 tttttcaact tgaaaaacag aatcaagaac agttccatca acccatttca cgattgctac
     1501 aactttatct ttaattagtt ttggttttgc tggtgcaccg ccgcaaattt catcaacttc
     1561 ttttttgatt tgctttatgt ctttaattgg tagtcctgaa cctttaactg cttttagtaa
     1621 atcttttctt cttggattaa ttgcaattcc tctttctgta attataacat caatcaattc
     1681 tccgggtccg caaattgttg taacttcatc aacgataaca ggaatacgat ctctgaatga
     1741 gggaattgca agtattgtgc attttgaata taaacaattc tgccagccgc caataccatg
     1801 taataaatat ccatcggaat gagtaacaac atttgcatta aacttcacat caacttcagt
     1861 cgcaccaaga actgcagtat caactatcga agcaaaatta cctttaccat gaaaattgta
     1921 acttgtgaat ggggaagtgt ttacgtgatt tgcattttct cgcatacttc taacaccttc
     1981 aagatcaaaa gtttgtccgt cgaggatgta atctgttaat ccttcttcga gcatttcaac
     2041 caaatatttc gta
//
LOCUS       DASWBY010000016         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_301630, whole genome shotgun sequence.
ACCESSION   DASWBY010000016 DASWBY010000000
VERSION     DASWBY010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301630"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1068
                     /locus_tag="VF870_00320"
     CDS             121..1068
                     /locus_tag="VF870_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX9250648.1"
                     /translation="MGREKKKNKITGGIIVATGKTAGKTAGKNGLYPLLKIGSITAVK
                     RIVLTFQQVGITPIVVVTGHKAEEIERDLADYGVIFLRNEQYENSQMFDSAKIGLDYL
                     YNKCDQVIFNPVNIQMFSPGTIKRMMECDEKLVSPSYHGKAGHPLLVSTKLIPRILKY
                     DGNMGMRGAIQNIGIQKKLMDIEDEGILHDTDDIDRLDQLLKKHNQHILHPFVRISIE
                     KESLFFNSRTKLLLILIQETQSVRNACKHIALSYSKAWNMLNQLEQELGYAVVERKHG
                     GSNGGKTYLTKEGIEFLKKYQQFEENVRKYAKDEFDRLF"
     gene            1263..>1588
                     /locus_tag="VF870_00325"
     CDS             1263..>1588
                     /locus_tag="VF870_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX9250649.1"
                     /translation="MQVNGVIVAAGLSSRMGEFKPLLKLTEKTMIEHSIDSMFNAGVN
                     QVIVVLGYRALEVEVLLRNKYDCSRLVFTHNLRYAETDMLESVKIGICALDTCDAFYL
                     LPGDMPA"
BASE COUNT          563 a          217 c          339 g          469 t
ORIGIN      
        1 tatttgggac tgcgggcata attggtggtc caataggaag tggtgcctct gcaaggccta
       61 gcccgcccac cgcagggcca ctagtactaa aattgacact acacggacag atgaggagat
      121 atggggaggg aaaaaaagaa aaataaaata acaggtggca taattgttgc cacaggaaaa
      181 acagcaggaa aaacagcagg aaaaaacgga ttatatccac tattgaagat cggttccatt
      241 actgcagtaa aaagaattgt tttaactttt caacaggttg gaattactcc aattgttgta
      301 gttactggac acaaagctga agaaattgaa cgcgatttag ctgattatgg ggttatattt
      361 ttgcgaaatg agcagtatga aaattcgcaa atgtttgatt cagcaaaaat tggactggat
      421 tatttgtata ataaatgtga tcaggtaata tttaatccag tgaatattca aatgttttca
      481 ccagggacca ttaagcggat gatggaatgt gatgaaaagc tagtttcacc atcttatcat
      541 ggaaaagctg gtcatccact tttggtatct accaaattaa ttcctagaat tttaaaatat
      601 gatgggaata tgggaatgcg tggagctatt caaaatattg gaattcaaaa gaaattgatg
      661 gatatagaag atgaaggtat tctccatgat acagatgaca ttgatagatt ggatcaactt
      721 ttaaaaaagc ataatcagca tatactgcat ccttttgtta gaataagtat tgaaaaagaa
      781 tctctgtttt ttaactcaag aacaaaattg cttttgattt tgattcagga aacacaatct
      841 gtacgaaatg catgtaaaca tattgcgttg tcttatagta aagcatggaa tatgctcaac
      901 cagttggaac aagagctagg gtatgcagta gttgaaagaa aacacggtgg tagcaatggt
      961 ggaaaaactt atttgacaaa agaaggcatt gaatttttaa aaaaatatca gcaattcgaa
     1021 gaaaatgtac gtaagtatgc aaaagatgaa tttgatagat tattctaaga atttagaatt
     1081 ttatgcaaag caagttcttt tgacaaagaa tttgataaaa agtacagtac cttttataat
     1141 aattataaca ataaaatgtt taattgctat aaaatggcaa attgcttctg caaaagatgg
     1201 ttgcagtgaa ggggcatgtg aatcactgta gcacatccat atttagaaag cgaggtgtgt
     1261 gattgcaagt taatggcgta atagtagctg ctggtttatc tagtcgtatg ggagaattta
     1321 aaccactttt aaaacttaca gaaaaaacta tgattgagca tagcattgat agcatgttta
     1381 atgctggtgt aaaccaagtc attgtggtat tgggatatcg tgcactggaa gttgaagttc
     1441 tgctgagaaa taaatatgat tgctcgcgat tagtttttac acacaacctt agatatgcag
     1501 aaacggacat gttggaatct gttaaaattg ggatttgcgc acttgatacc tgtgatgcct
     1561 tttatttact tccaggggat atgccagc
//
LOCUS       DASWBY010000017         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_113588, whole genome shotgun sequence.
ACCESSION   DASWBY010000017 DASWBY010000000
VERSION     DASWBY010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113588"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1615
                     /locus_tag="VF870_00330"
     CDS             266..1615
                     /locus_tag="VF870_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015078275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9250650.1"
                     /translation="MFNIISRLQCLKTCLSSTAISQLNVIVTALLAMTGRVTMLGISR
                     WAGEGGSYRTVQRFFNTPIAWVKVHWCLIRHCFLSPDDVFIMAGDETVVTKSGKITHG
                     IDRFFSSIYEKVVPGLSFFTFSLISTKHRASFPIMIQQNIRSEEKTICTEDLSSTEHS
                     KEDQNRNGPKKKIGRGRPKGSKDKNKENVDLSPYLCFIQSMLKKVLLIIGNRTILTYM
                     VLDGAFGNNNAFQTVKQCGLHLISKLHRNSALYFPYSGKYSGRGRMKKYGDRVDYDNI
                     PKDYLKETTCEESIQTETYQMTLLHKLFAQKLNVVIIVKTNLRTLARSHVILFSSDLD
                     LPYGKLIDYYSLRFQIEFNFRDAKQYWGLEDFMNVKETPITNAANLAFFMVNISHALI
                     DEIRKHNPLFGIQDLKAYFRAGKYLYETLKLIPEKPDPILIQQLFAEITKIGSINTP"
BASE COUNT          585 a          346 c          329 g          572 t
ORIGIN      
        1 tgcaccctct tgcatggatg tcacaagcac tcttacccct gccacaatac agaatttctc
       61 ctttacagtc gtgagggtaa ctcgggcaat cagggcagat gcatttattc ctattttcct
      121 cagtatcttt aactatcata accccaatat tttaatacta tccaaagatc aataccttcg
      181 ccacattgct atatctataa gataagatga attaattctg ggatatatca cgggttctat
      241 tctatgatat atcccaggaa cttgtatgtt caatattatt tcacgtcttc aatgtttaaa
      301 aacctgtctg agttcaacag ctatttctca gttgaatgtc attgtgactg cactattagc
      361 catgaccggt cgagttacca tgcttggtat ttctcgttgg gctggagaag gtggaagtta
      421 tcgtactgtt cagcgatttt tcaacactcc tattgcatgg gttaaagttc actggtgtct
      481 aattcgtcat tgtttcctat caccagatga tgtttttatt atggcaggtg atgagaccgt
      541 agttactaaa tcgggcaaaa taacccacgg catcgatcgt tttttctcat ctatatatga
      601 gaaagtggta cctggattat cttttttcac attttcgttg atcagtacaa aacatcgggc
      661 atcctttcca ataatgattc aacaaaatat tcgttctgaa gaaaaaacaa tatgcacaga
      721 agacttgagt tctacagaac atagcaaaga agatcaaaat aggaatggac ccaagaagaa
      781 aattggcaga ggtcggccaa aaggcagtaa agacaaaaac aaggagaatg ttgatctatc
      841 accatatctc tgttttattc agagcatgct gaaaaaagtt ctgctgatca ttggaaatcg
      901 cactatcctc acttatatgg ttctagatgg tgcgtttgga aataataatg cctttcaaac
      961 ggtcaaacaa tgcgggttgc acttgatttc caaacttcat cgtaactctg cactatattt
     1021 tccttattca ggtaaatata gcggtcgtgg taggatgaaa aaatatggcg atagagtcga
     1081 ttatgacaat attccaaaag attatttgaa agaaactacc tgcgaagagt caattcaaac
     1141 cgaaacatat caaatgacat tgctgcataa gctattcgct cagaagctaa atgttgtcat
     1201 tattgttaaa acgaatctca gaacccttgc aagatcccat gtaattttat tcagcagcga
     1261 tctggatttg ccctacggga agttgattga ttattacagt ctgagatttc agattgagtt
     1321 taatttccga gatgcaaagc aatattgggg attggaagat tttatgaatg tcaaggaaac
     1381 gccgatcact aacgcggcca acctggcatt tttcatggtg aacatatctc acgctttgat
     1441 cgatgagatt cgtaaacata acccactatt tgggattcaa gatctcaaag catattttag
     1501 agcagggaaa tacctctatg aaacattaaa attgataccg gaaaagccag atccgatttt
     1561 aattcagcaa ttgtttgcag aaataacgaa aataggtagt attaatactc cttaatggcg
     1621 aaggtattga aatatagttg atatttcgta agtaacacta ccgagttgca ctttcataga
     1681 ttgcgcaacc aaccaataga atcctactat aatcgctaaa atgcctgttc ccgagaaaat
     1741 ctccctgtct ttcataataa tttgtatcca tttcatattt ttcagagata ttaaaaaatc
     1801 ttatttcaat tgataaatta tgacgtcctt at
//
LOCUS       DASWBY010000018         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_301740, whole genome shotgun sequence.
ACCESSION   DASWBY010000018 DASWBY010000000
VERSION     DASWBY010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301740"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VF870_00335"
     CDS             complement(<1..275)
                     /locus_tag="VF870_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX9250651.1"
                     /translation="MSWHEMLNVILLNLRTIVKVTILSTIFLFIILLFIYPRTYQATV
                     SILPPEQENSLGGFGALLGNGDFSNLLGGGLSSASSQLFVEILKSRTA"
     gene            complement(279..>1808)
                     /locus_tag="VF870_00340"
     CDS             complement(279..>1808)
                     /locus_tag="VF870_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX9250652.1"
                     /translation="SFAQLFPTYPERSFNSFSDSSFNKSMQASEGIINPAEYKVGPGD
                     NIFISISGVEERDFNLLINHEGYLYIPRIGAVDLRNKTLEEAKVLIRSTLEKNFKNVD
                     IYIALGEVRKIKVSLIGNVKNQSTIVLTSNSRLQDLLKSTAGINSSSDIRNIKIISKD
                     GSTGNYDLLSFFRKGDLKQNPYLKDGDAVIIEKVNKIVSIMGLVLYPATYEYRENETV
                     NDLINLAGGLTYKARKDSIELIRFDEDGINQFSQYFSYDFIQQNPIELKFSDMVIVRE
                     ITTYYDEQWVSIEGKIKYPGLYKIVKNKTKLTEVINQAGGFLEDASLVDATLFRSDAD
                     SSYDPEFERIKLIPRADMTDDEYDYFKAKSRQRYGKVVVDFKALFRGGQLSEDVILKR
                     KDVINIPERKNYISIIGQVVNPGNLIYDPNLKVNDYIQLAGGFSWRAIEGDVRVIKVN
                     TGEWVDADDIVRLDPGDTIWVLEDPPGPKFWDVFLTSLTVLAQLAAIVAASVAVVVAT
                     R"
BASE COUNT          575 a          360 c          261 g          612 t
ORIGIN      
        1 gccgttctgc ttttaagaat ttcaacaaaa agttgagaac ttgcggatga cagaccaccg
       61 ccaagcagat ttgagaaatc gccattacct aataatgcac cgaagccacc taaactgttt
      121 tcctgctcag ggggaagaat tgaaacagta gcctgataag ttcttggata aatgaataat
      181 aaaattataa ataaaaaaat agttgacagg atggttactt taactattgt cctcaaattc
      241 aataaaataa catttaacat ttcatgccag ctcatttatt acctcgtagc tactactaca
      301 gcaactgatg cagcaacaat tgcagcaagt tgtgctaata ctgttaaaga tgttaaaaat
      361 acatcccaga atttaggacc tggtggatct tctaaaaccc atattgtgtc cccaggatct
      421 aaacgaacta tatcatctgc atctacccac tcacctgtat ttacttttat tactctaaca
      481 tctccctcaa tggctctcca actaaaaccg ccggctaatt ggatataatc gttaaccttg
      541 agatttggat cgtaaatcaa attaccagga ttaacaacct ggccaataat agaaatataa
      601 ttttttcttt caggaatgtt tataacatct tttcttttta agattacatc ttcacttaac
      661 tgaccacctc tgaacaaagc cttaaagtct acaactacct taccgtatcg ttgtcttgat
      721 tttgctttaa agtaatcata ttcatcatcc gtcatatctg cacggggaat taatttaatt
      781 ctttcaaatt ctgggtcgta agatgaatct gcatcgcttc taaaaagagt tgcatccaca
      841 agtgaagcat cttctaaaaa tcctccagcc tgatttataa cttccgtaag cttggtttta
      901 ttttttacaa ttttatacaa acctggatac tttattttgc cttctatact aacccattgc
      961 tcatcataat aagtcgtaat ctcacgaact attaccatgt cgctgaattt taattcaatt
     1021 gggttttgtt ggataaaatc ataagaaaaa tattgactga actgattgat gccatcttca
     1081 tcaaatcgga ttaattcaat tgagtctttt ctagctttat atgttagtcc cccagctaga
     1141 ttaatcaaat cattaactgt ttcattctcc cggtattcat acgttgccgg ataaaggaca
     1201 agacccatga tggaaacgat cttatttact ttttcaataa taaccgcatc cccatctttt
     1261 aaatatggat tctgtttcaa atcccctttt ctaaaaaacg aaagcaaatc ataattgccg
     1321 gtactgccat ccttagaaat aattttaata tttctgatat ctgatgaaga gtttataccc
     1381 gctgtgctct ttaagagatc ctgcaatctt gaatttgatg ttaaaacaat tgtcgattga
     1441 ttttttacat taccgatcag cgaaactttt atctttctaa cctcaccgag cgctatataa
     1501 atatcaacat ttttaaagtt tttttctaaa gttgatctta tcaaaacttt tgcttcttca
     1561 agtgttttat ttcttaaatc aacggcaccg attcttggga tgtataaata tccttcgtgg
     1621 ttgatcaaca aattaaaatc tctttcctca actccgctaa tagaaataaa tatattatct
     1681 cccggcccaa ctttatattc ggcgggatta attattcctt cggatgcctg catggatttg
     1741 ttgaaggaac tatcagaaaa ggaattgaat gatctttcgg gataagttgg gaataattga
     1801 gcaaaact
//
LOCUS       DASWBY010000019         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_565107, whole genome shotgun sequence.
ACCESSION   DASWBY010000019 DASWBY010000000
VERSION     DASWBY010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565107"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VF870_00345"
     CDS             complement(<1..689)
                     /locus_tag="VF870_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX9250653.1"
                     /translation="MESSLTLKHSILKKFKFNYDDCAIFENYIVELLGYDVDNVPEPV
                     LETIKFLLDRIPEKVSLQSGYKIFNPKKVKLNGDSFFIDNRLFNCGKIIYSSLKNSET
                     IAFLISSIGEEVELWSKHFMDNNEMLKGYLIDKIASELVEQLADKTELMLEDELKEIE
                     LEATNRYSPGYCGWSVADQQNLFSLLPAKFCGVSVNDNSMMIPIKSVSAVIGVGKNVE
                     KKNYECSICEI"
     gene            complement(695..1366)
                     /locus_tag="VF870_00350"
     CDS             complement(695..1366)
                     /locus_tag="VF870_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX9250654.1"
                     /translation="MNEILQQLSLCIEKGRVNKDSNYPPDMKGMPGAVELTHQALDNG
                     IPAQDVLNLGFLPGMKIVGDKFKAGTIFLPEVLISAKAMSSSMELLKPYFKSGEINYK
                     GKVIMGTVAGDLHDIGKNIVKMVLEGGGWQVVDLGINVSAEKFVEAIKTNSAKVVGLS
                     ALLTTTMQNMNEIIRRIKSETNDVLVIVGGAPVTKAFADSINADEYFVDPQGMLDYLN
                     NKTEL"
     gene            1559..>2487
                     /locus_tag="VF870_00355"
     CDS             1559..>2487
                     /locus_tag="VF870_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9250655.1"
                     /translation="MLKPKTELVRGLTLTAAIMIVAGSMIGSGIFRKPATMAAQLMSP
                     ELLLIVWLVAGIITFIGALCNAEVAGMIDATGGQYVYFQKMYGNFVSYLYGWSVFAVI
                     QTGAGAAIAYVFGEYLGYFIKYPQLPESLQKFSIFIPIVGNIFPFAEFGAKASAILCI
                     LFLTGINYVGVIFGGAVQSFVTFIKIASIMLLSFLLLASGSGSLANISTGFSMPTSST
                     SNIISLFGLAIAGAFWAYDGWNNLTFVAGEIKQPQRNVPLGLLFGTLIVVIVYMLVNV
                     AYLYVLPIEKMAQSPLVAATAAEFVFGSKGAIL"
BASE COUNT          763 a          478 c          385 g          861 t
ORIGIN      
        1 tctatttcac aaatagaaca ttcataattt ttcttttcaa cattttttcc aactccgatt
       61 actgcactaa cagattttat gggtatcatc atggaattat catttactga tactccacaa
      121 aactttgctg gcagcagcga aaataaattc tgctgatctg caacactcca tccgcaataa
      181 cccggactgt agcgatttgt ggcttcgagt tctatctcct taagttcatc ttctagcatt
      241 aattctgttt tatcagcaag ttgttcaacc aattcagagg caattttatc aatcaaatat
      301 ccttttaaca tttcattatt atccataaaa tgtttggacc aaagttctac ttcctcaccg
      361 atggaagaaa taaggaaagc aatagtttca gagtttttta aagatgaata aataattttc
      421 ccgcagttaa atagcctgtt atctataaaa aaactatcac cgtttaattt tacttttttt
      481 ggattgaata ttttgtaacc gctttgcaag ctaacttttt ccggaattct atcaagtaaa
      541 aatttaatag tttctaaaac aggttccggg acattatcaa catcataacc caataattct
      601 acaatataat tttcgaaaat agcacaatcg tcataattga atttgaattt cttcaggata
      661 gaatgtttta aggttagtga actttccata aggattataa ttcggtttta ttattaagat
      721 aatcaagcat tccttgcgga tcaacaaagt actcatcagc atttatggaa tcggcaaatg
      781 cttttgtaac aggtgcacct ccaacaataa ccaaaacgtc atttgtctct gatttaatcc
      841 ttcttattat ttcattcata ttttgcattg ttgtagtaag taatgcactt aatccaacaa
      901 ctttagcaga attagttttt atagcttcaa caaatttttc agcacttaca ttaattccca
      961 aatccacaac ctgccagcct ccgccttcca aaaccatttt aacaatgttc ttcccaatat
     1021 cgtgaagatc accggcaacc gtacccatta taactttacc cttgtaattt atttcacctg
     1081 atttgaaata tggttttagc aactccattg aactgctcat agcttttgct gagatgagaa
     1141 cttcaggaag aaagatagtg ccggctttaa atttatcacc gactattttc attccaggta
     1201 aaaacccaag attaagtaca tcctgagcag ggataccatt atcaagagcc tggtgagtta
     1261 attcaacagc tccgggcatt cctttcatat ctggtggata gtttgaatct ttgtttactc
     1321 ttcccttttc aatacaaaga ctaagctgct gaagaatttc attcattgtt aaaatctatc
     1381 gttaattgat ttttaatcgt aaccaaaaat aaacaattgt attgagatag agaattaaca
     1441 tattttttta ctaagataat tgttcctttt ataaaactta tttatgttca ctcgcaaata
     1501 attcttaaga ttttaatcaa aggaaaaact atttacatca caaacaatat cctgccagat
     1561 gcttaaacca aaaactgaac ttgtccgggg tctaacatta actgcagcta ttatgatcgt
     1621 tgcaggctca atgatcggat caggaatttt tagaaaacct gcaacgatgg ctgctcaatt
     1681 aatgtcaccg gaattgcttt taattgtttg gctggttgcg ggcataatta catttattgg
     1741 agctctttgc aatgcagaag ttgcaggcat gatcgatgca accggcggac aatacgttta
     1801 cttccaaaaa atgtacggca actttgtatc atacctttac ggctggtctg tttttgctgt
     1861 tatccaaaca ggcgctggag ccgcaattgc atacgtattt ggagaatacc ttggttactt
     1921 tattaaatat ccacaactac ctgaatcgct gcaaaaattt tcaatattta ttccaattgt
     1981 tggaaacatt ttcccctttg cagaatttgg agccaaagca tcagcgattt tgtgtattct
     2041 ttttttaacc gggatcaatt atgtaggagt aatttttggt ggtgcagttc aatcatttgt
     2101 taccttcata aaaattgcct cgataatgct tttatcattc ttattattag caagcggctc
     2161 cggcagtctt gcaaatatct ccaccggttt ttcaatgccc acatcatcaa cttcaaatat
     2221 tatttctttg ttcggtctag ctattgcagg tgcattttgg gcttacgatg gatggaataa
     2281 cttaaccttt gttgcaggcg aaataaaaca accccaaaga aatgttccgc tgggactatt
     2341 atttggaaca ttaatcgtag taattgttta tatgcttgta aacgttgcat atctatatgt
     2401 tttgccgatt gaaaaaatgg cccaatctcc tcttgttgca gctactgcag ctgaatttgt
     2461 tttcggatca aagggagcaa tattaat
//
LOCUS       DASWBY010000020         8149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_640251, whole genome shotgun sequence.
ACCESSION   DASWBY010000020 DASWBY010000000
VERSION     DASWBY010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640251"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VF870_00360"
     CDS             <1..462
                     /locus_tag="VF870_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9250656.1"
                     /translation="DQSITYVRRAIANGRIALFQGIFSAPGTVNDVKKDCEKAIQLGN
                     GDNYVQALAHYVLGRTHLKVCENSYFVRLPLGLGWGDTEEALRLLETANKLKPNFRMF
                     MYELAKAYVEEDEYDKAKELLKKVEKAPKIDEDDDIVLADAKKLYEEIKNE"
     gene            571..1296
                     /locus_tag="VF870_00365"
     CDS             571..1296
                     /locus_tag="VF870_00365"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX9250657.1"
                     /translation="MNFSPAFLNKLSESNKIVFFTGAGISAESGIPTFRGEGGIWNKL
                     KPEELANFNAFMRNPQMVWEWYNHRKKIIHEAKPNAGHLAIAQMQNLFNEVTVVTQNI
                     DNLHRRAGSKNIFELHGNIERNYCINCKTFYNEELDFSKGVPKCKCGGLIRPDVVWFG
                     EYLPEDQFEGGELAALNSDMLFVVGTSAVVYPAAGLVHAAKRAGSFIVEVNVVETEIS
                     SICDESFFGEAGKVLPEIVNQIK"
     gene            complement(1363..1635)
                     /locus_tag="VF870_00370"
     CDS             complement(1363..1635)
                     /locus_tag="VF870_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX9250658.1"
                     /translation="MFDIILLLIIAGITGGIGRSLVGFKRGGCIISIIVGFIGAYIGT
                     ILAREFNFPDFWSFEIRGINYPIVWSIIGAVVFTAILSLIVPKKNN"
     gene            1767..2705
                     /locus_tag="VF870_00375"
     CDS             1767..2705
                     /locus_tag="VF870_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9250659.1"
                     /translation="MNRRRFLRNISLASTGLLFVNEKLHAKQPPSLPNFKPDPTSWEN
                     DEINISWIGHATVLINFYGTTILTDPVLFERVGVNIFGMTFGPSRFTYPALTVDEIPK
                     PDIMLLSHAHMDHMDYETLSTIVSKYPNQIDCITAYNTADVIADFEWKSLKEIDWNEE
                     TELPGIKFKALEVKHFGWRYPWEKDRSKGYMSDGRSFNAVLIEKNGKKILFGGDTAYH
                     ELFLPLKNENIEIAIMPIGAYEPWRKNHCNPEEALIMSSEHVGAKYFIPIHTKTFKQG
                     SEPIDEPLTWLKNSVVNYDIKLGLDDIGKTFTLPNL"
     gene            2726..3292
                     /locus_tag="VF870_00380"
     CDS             2726..3292
                     /locus_tag="VF870_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9250660.1"
                     /translation="MQSIQQLKDNFKADTETVIKDLEKFKNLSINQINWKPFAEGWGI
                     AECIDHLFVTNKLYLTEFEKQFAQKQITTDCANTKSKHKLLSKFIIKSVNPANVKKVK
                     TFPVFMPSKSNHSHDVFEEFKRIQTDLINIVLTYKDLDFNRYVMSSPAAKIIKENFCD
                     VLEIIRLHDRRHFNQAERVLNHSNFPEN"
     gene            3296..3829
                     /locus_tag="VF870_00385"
     CDS             3296..3829
                     /locus_tag="VF870_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX9250661.1"
                     /translation="MSKLEKGYIQIYTGNGKGKSTAAIGQAVRAAGYGLKTYIAQFMK
                     EYPYNELKSLENLKEWITIEQFAGDDFVYRKEMPSKVEMDKARRGLETAKAKMLKGQY
                     DLIILDEILVSIHFGLFNDEEVMTFMKMKPENVELILTGRYCPDKILEAADLVTEMKV
                     IKHYYQEGVLARKGIES"
     gene            3958..4593
                     /locus_tag="VF870_00390"
     CDS             3958..4593
                     /locus_tag="VF870_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX9250662.1"
                     /translation="MKEIKQSHFLGFYNALLEGDRDKCNHLVQSLIDEGADLKDIYVE
                     LFQKSLYRIGKLWDHNKISIPEEHLATQIIESLISRFAPVGKSDQQKKVVVTCIDKEF
                     HNIGAKMVSNVFELKGWKTYYLGASVPTKEIVKYVKTIEPEIVALSWSLYLNLGRFLE
                     VVDHLTRFFPTRKIIVGGQALAENSDKVLKKYKNVKYIESLDSLELYLKKG"
     gene            4690..5355
                     /locus_tag="VF870_00395"
     CDS             4690..5355
                     /locus_tag="VF870_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX9250663.1"
                     /translation="MDNISQEDYLTTIYRNLDEDGEIKPNLLASKLEISNAAVTDMLR
                     KLSRDGFVDYQKYKGIKLTSEGELYAKNMLRRHRIWEVFLHQILAMPWDKVHEEAEKL
                     EHSSSDELINLLEEFLEYPEVDPHGYPIPDKNGKIKKSKSVVAITELSENDLAKVVRV
                     NDAVKNLLSYVTKIGISLGKEIMIKDKLEYDGSVLLMLDNKEVNISNKIASNIFVEKM
                     KQV"
     gene            5357..6379
                     /locus_tag="VF870_00400"
     CDS             5357..6379
                     /locus_tag="VF870_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HEX9250664.1"
                     /translation="MNEPLMFLIFGSITILIFVLLFYPEKGIIAVWKKSIYANKKVLI
                     EDALKYLYNCEYNNTTCSLNGIAGNLSISADDATDIISRLESMGLVSAKKDELTLTSD
                     GRSYALRVIRVHRLWEKYLADETSVTEKEWHQQAEELEHKLTLEQADQLAAQIGNPVF
                     DPHGDPIPTASGVLPVKKGKTLTEMKVGEFGNIIHVEDEPHAIYSQILAEGLYPGMQI
                     RMMEVSDRRLKFVANGEECVLSPLIAKNITVGVLKLEKQVQGKFKTLSSLKIGEEGTI
                     LGIAKSLRGQQRRRLMDLGIVPGTKIEAELKSFTGDPVAYRVRGTTVALREQQTEKIY
                     LLNDEK"
     gene            6383..7186
                     /locus_tag="VF870_00405"
     CDS             6383..7186
                     /locus_tag="VF870_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HEX9250665.1"
                     /translation="MINTELNNCANCPVHNMGNLKKLGIDMSSFDFVVALAGNPNTGK
                     STVFNYLTGLRQHTGNWPGKTVTRAEGGFEYGEKKFKLVDLPGTYSLLSTSTDEEVAR
                     DFILFGQPDVTVIVVDATRVERNLNLVLQILEITDRAVLCLNLMDEAKRNGIKVDERT
                     LSKELGIPVIPASARQGEGMNELLNTIHDVATGKYICKPYRIKTKSAELNSAIEKLSK
                     EIELEYPGLPNLRWVALRLLEGDQSIIDAIRSGDLGNLQKNFILSEVEG"
     gene            7197..>8149
                     /locus_tag="VF870_00410"
     CDS             7197..>8149
                     /locus_tag="VF870_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX9250666.1"
                     /translation="MNDNLKRESILATANNLRWDVGDNLHDTLMESIYENATSISSKV
                     VTYPDKKPAFSLDRTLDKILTSRYLGFPIMFLILAVVFWITIVGANVPSGLIANLLVD
                     TLHPALKTLANSIGFPWWLSGVLIDGAYLAMAWVVSVMLPPMAIFFPVYTLLEDFGYL
                     PRVAFNMDPLYKRVGAHGRQALTMAMGFGCNAAGVVATRVIDSPRERLIAIITNNFSL
                     CNGRWPTQILIATIFIGGAAPAYLAGIASAGAVVFIAVLGIVFSLIVSWGLSKSLLKG
                     EASAFSLELPPYRPPRILQTLYTSLIDRTIFVLWRAIVFAVP"
BASE COUNT         2861 a         1207 c         1595 g         2486 t
ORIGIN      
        1 gatcaatcta taacttatgt tagaagagca attgcaaatg gaagaatagc tttatttcaa
       61 ggaatcttct ccgcgccggg gacagtaaat gatgtaaaaa aagattgtga aaaagccatt
      121 caattaggaa atggagataa ttatgtgcag gcacttgctc attatgttct gggtagaact
      181 cacctcaaag tttgtgaaaa ttcttatttc gttagactgc cattaggtct tggatggggt
      241 gatacagaag aagcattaag acttcttgaa actgctaata agcttaaacc caatttcagg
      301 atgtttatgt atgaactagc aaaggcttac gttgaagaag atgaatatga taaagcaaaa
      361 gaacttttaa aaaaggttga aaaagcacct aagattgatg aagatgatga tatagttttg
      421 gctgatgcaa aaaaacttta tgaagaaatt aaaaacgagt aatgttctta gagtttctta
      481 gtgtcctttg tgtcttagtg gtgtgtgttt ttaccactaa gacactttga acacttggtt
      541 taacttagaa aaaaaatcag gaattctaaa atgaatttca gtccggcatt tcttaataaa
      601 ctttccgaat cgaataaaat tgtttttttt acaggtgcag gtatttccgc tgaatcagga
      661 attccgacgt ttcgcggtga aggcggaatc tggaataaat taaaacctga agaacttgca
      721 aactttaatg catttatgcg caatcctcag atggtgtggg aatggtacaa tcatagaaaa
      781 aagattatac acgaagcaaa acctaatgca ggacatttag caattgcgca gatgcaaaat
      841 ctgtttaatg aagttacagt agtaactcaa aatattgata atcttcatcg cagggcaggg
      901 agtaaaaata tttttgaact tcatggaaat attgaaagaa actattgtat taattgcaaa
      961 acattctaca atgaagaatt ggatttttca aaaggagtac ccaaatgtaa atgcggcggc
     1021 ttgatccgtc ctgatgttgt ttggtttggg gaatatcttc ctgaagatca atttgaaggc
     1081 ggggaacttg cagcacttaa tagcgatatg ttgtttgttg taggtacctc agcagtagta
     1141 tatcccgcag caggattagt tcatgcggca aaacgtgcag gttcatttat tgttgaagta
     1201 aatgttgtag agacagaaat ttcatcaatt tgtgatgaat ctttttttgg tgaagcggga
     1261 aaagtgttac ctgaaattgt aaatcaaata aaatgatttt tttaaatcat tgcgaactta
     1321 ttttgtaaaa gagtagaagc ttaaacaatt ttaacttgat atttaattat tcttcttcgg
     1381 aacaatcaag cttaaaatag ctgtaaaaac cacagcaccg ataatagacc aaacaatagg
     1441 gtaattaatt ccgcgaattt caaacgacca gaaatcaggg aagttaaatt cacgagccaa
     1501 aattgttccg atgtaggcac caataaatcc aacaataatt gaaataatac aaccaccccg
     1561 tttaaatccg acaagtgaac gcccgattcc gccagttatt ccggcgataa ttagtaaaag
     1621 tattatatca aacatgattt gcctcaaact aaatttgtgt ggtagaaaaa taggttttat
     1681 tctgataaaa ttcttgtaat atttaaatta gaactattat tagaaatatt tggttatatg
     1741 attgtaaata ataacgagga ctgattttga atcgcagaag atttttaaga aatatttcac
     1801 ttgcatcgac gggcctttta tttgttaatg aaaagctgca cgctaaacaa ccgccatcat
     1861 tgccaaattt caaaccagat ccaacaagct gggaaaatga tgaaattaat atttcctgga
     1921 tcgggcatgc cactgtacta attaactttt atggaacaac tatccttact gatcctgttt
     1981 tgtttgaacg ggttggagtt aacatcttcg ggatgacatt cggaccgagc agatttacat
     2041 atccggcatt aaccgtagat gaaattccta aaccggatat catgttactc tctcacgctc
     2101 atatggatca tatggattat gaaacacttt ctacgattgt tagtaaatat cctaaccaaa
     2161 tcgattgtat aactgcttat aacactgccg atgtaattgc tgattttgaa tggaaatcat
     2221 tgaaggaaat tgattggaat gaagaaaccg aattacctgg aataaaattc aaagccctgg
     2281 aagtaaaaca ttttggctgg cgttaccctt gggagaagga tcgatctaaa ggttatatga
     2341 gtgacggcag aagttttaat gcagtgctta ttgaaaaaaa cggaaagaaa attctttttg
     2401 gcggagatac agcttatcat gaattattct tgcctctaaa aaatgaaaat atagaaattg
     2461 caataatgcc gattggtgca tatgagccgt ggagaaaaaa tcattgtaat ccagaggaag
     2521 ctttaataat gtcaagtgaa cacgtagggg caaaatattt tattccaatt catacaaaaa
     2581 catttaagca gggcagtgag ccaattgatg aaccgttaac ctggttaaaa aactcagtag
     2641 taaactacga tattaaactt ggtctggatg atattggaaa aacgtttaca cttcccaatc
     2701 tttaatcgat aaattaaatt tcattatgca atctattcaa caattaaaag ataattttaa
     2761 agctgatact gaaactgtta taaaagattt agaaaaattt aaaaatcttt ctataaatca
     2821 gattaactgg aaaccatttg ctgaaggctg gggtattgcc gaatgtatag atcacctttt
     2881 cgtaactaat aaactttatc taacagaatt tgaaaagcaa tttgctcaaa aacaaataac
     2941 cacagattgt gcaaacacaa aatccaaaca caaattgttg agtaagttta taattaaatc
     3001 agtcaatccg gcaaatgtta aaaaagttaa aacattccct gtttttatgc catctaaaag
     3061 caatcattca catgatgtat ttgaggaatt taaaagaatc caaactgatt tgataaatat
     3121 tgtactcact tacaaagatt tggattttaa tagatatgtt atgtcatctc ctgcagcaaa
     3181 aattattaaa gaaaactttt gtgacgtact tgaaataatt cgtttgcacg ataggaggca
     3241 ttttaatcaa gctgaacggg ttctgaatca ttctaacttt cctgaaaatt aatatatgag
     3301 caaattagaa aaaggttaca tccagattta cacgggtaat ggtaaaggta aatcaactgc
     3361 cgcaataggg caggctgtaa gagctgcagg ttatggactt aaaacctaca tcgcacagtt
     3421 tatgaaggaa tatccctaca acgaattaaa aagtttggaa aatttaaaag aatggattac
     3481 gatagaacaa tttgcaggag atgattttgt ttatcgtaaa gaaatgccaa gcaaagttga
     3541 gatggataaa gcaagacgcg gacttgaaac agcaaaagca aaaatgttga aaggacaata
     3601 tgatttaata atccttgatg aaattttagt ttcaattcat tttggattat ttaatgatga
     3661 agaagttatg acctttatga aaatgaaacc cgaaaatgtt gaattgattt taaccggaag
     3721 atattgtccc gataaaatat tggaagccgc tgatcttgtt actgaaatga aggttataaa
     3781 acattattat caggaaggtg ttctagcaag gaaaggaatt gaatcataat tttatatcaa
     3841 ttttttagat ctgattttgt tcggacaaag tgtacatata aaacataaaa attagatttt
     3901 ggaacttttt taagagaaaa tgaattatta gtttatactt agtctattgg aaacactatg
     3961 aaagaaataa aacaatctca ttttcttggc ttttacaatg ctctgcttga aggagataga
     4021 gataaatgta accatcttgt tcaatcctta attgacgaag gagctgatct aaaagatatt
     4081 tatgtagagc tttttcaaaa atctctttac agaataggaa aactttggga tcataataaa
     4141 atatcaatcc ctgaagaaca tttggcgact caaattattg aatctcttat cagtcggttt
     4201 gctccggttg gaaaatcaga tcagcagaaa aaagtggttg ttacttgtat tgataaggaa
     4261 tttcacaaca ttggtgctaa aatggtgtcc aatgtttttg aacttaaggg atggaaaaca
     4321 tattatttag gcgcttctgt tcccaccaaa gaaattgtaa aatatgtaaa gacaattgaa
     4381 cctgaaatag ttgcactttc ctggagttta tatttaaatc ttggaagatt tttggaggta
     4441 gtagatcact taaccaggtt ttttccaacc aggaaaatta ttgtaggcgg ccaggcttta
     4501 gctgaaaatt ctgataaagt attgaaaaag tataaaaatg ttaagtacat agaatcgctg
     4561 gattctctag aattatatct taaaaaaggt taatgtgatt caatttacag gcaaagtttt
     4621 aagctttgcc ttttctttta ttgttttata aatttaagta cacttaaaaa tataattaga
     4681 ataacttaaa tggataatat ttcacaagaa gactatctaa ctactattta ccgtaacctg
     4741 gatgaagatg gagaaattaa gcctaatctt ttggcttcta aattagaaat ttcaaatgcc
     4801 gcggtaactg atatgctgcg aaagctttct cgggatggtt ttgtagatta tcaaaaatat
     4861 aagggcatca agcttacaag cgaaggggaa ttgtacgcga aaaatatgct tcgccgccat
     4921 agaatttggg aagtatttct tcatcaaata cttgcaatgc catgggataa agttcatgag
     4981 gaagctgaaa aattggaaca ttcatcttcg gatgaattga taaatctgtt ggaagaattt
     5041 ttggaatatc cggaagttga tccgcatggt tacccaattc ctgataaaaa cgggaaaata
     5101 aaaaaatcaa aatctgtagt agccataaca gaacttagtg aaaatgattt agcaaaagta
     5161 gttcgtgtaa atgatgctgt aaaaaatctt ttatcatatg taacaaaaat cggaatatca
     5221 cttggtaaag aaattatgat aaaagataaa ctcgaatatg acggatcggt tttgttgatg
     5281 ttggataaca aagaagtaaa catcagtaac aaaattgcat caaatatttt tgttgaaaag
     5341 atgaagcagg tataaaatga atgagccatt aatgtttttg atatttggat caataacaat
     5401 tttaattttt gtgttattgt tttatccgga aaaagggata atagctgttt ggaaaaaatc
     5461 tatatatgca aataaaaaag tattaataga agatgccctt aaatatttgt ataactgtga
     5521 atacaataac acaacctgtt cattaaacgg aattgccgga aatctttcaa tctctgcaga
     5581 tgatgcgaca gatattattt caagacttga atcaatggga ctggtttctg ctaaaaagga
     5641 tgaactgact ctaacctcgg acggaagatc gtatgcactt agagtaataa gagtgcatcg
     5701 tttgtgggaa aaatatctgg ctgacgaaac aagtgtaact gaaaaagaat ggcatcaaca
     5761 ggcagaagaa ttagaacaca aattaacact tgaacaagca gatcagttgg cagctcaaat
     5821 tggtaatcct gtctttgatc cgcatggtga tccaattcct acagcatcag gtgttcttcc
     5881 agtaaaaaag ggaaaaacat taacagaaat gaaagttgga gaatttggaa atatcattca
     5941 cgtagaagat gaaccgcatg caatttacag tcagattttg gctgaggggc tgtatcccgg
     6001 tatgcaaata agaatgatgg aagtttccga taggcgatta aaatttgtag caaatggtga
     6061 agaatgtgtg ctgtctccac ttatagccaa gaacataacg gttggtgttt tgaaacttga
     6121 aaaacaggtt cagggaaagt ttaaaactct ttcatcactt aaaattggtg aagagggaac
     6181 tatacttgga atagctaaat ctttgcgcgg tcagcagaga aggagactta tggatttagg
     6241 aatcgtaccc ggaacaaaaa tagaggctga gcttaaaagt tttaccggtg atccggttgc
     6301 gtacagagta agaggaacta cagttgcatt acgtgaacag caaacagaga aaatttattt
     6361 attgaatgat gagaaataat ttatgataaa cactgagcta aataattgtg caaactgtcc
     6421 cgtgcacaac atgggtaatc ttaaaaagct tggtattgat atgagcagct ttgattttgt
     6481 tgttgctcta gcaggaaacc caaacacagg taaaagcaca gtctttaatt atcttacagg
     6541 attaagacaa cacacaggca actggccggg aaaaaccgtc actcgtgcag aaggcggatt
     6601 tgaatacggc gagaaaaaat ttaagcttgt tgatcttcct ggaacatact cactgttatc
     6661 tacaagcaca gatgaggagg ttgcgcgcga ttttatttta ttcgggcagc cggatgtaac
     6721 agtaattgtt gtggatgcaa cacgagttga aagaaacctg aatctagttt tacagatttt
     6781 agaaattacg gatcgtgctg tattgtgtct taacctgatg gatgaagcta aaagaaatgg
     6841 aattaaagtt gatgagagaa ctttatcaaa agaattgggt attccggtta ttcctgcttc
     6901 tgcaaggcag ggagaaggaa tgaatgaact attaaataca attcacgatg ttgcaacagg
     6961 aaaatatatc tgcaaacctt ataggataaa aacaaaatcg gctgaattaa attctgcgat
     7021 tgaaaaatta agtaaggaaa ttgaattaga gtatcccgga ttaccaaacc ttcgttgggt
     7081 tgctttacgt ttacttgaag gtgaccagag cattattgat gcgatacgca gcggcgatct
     7141 aggaaattta caaaagaatt ttatattgag tgaagttgag ggatgaaaca aataaaatga
     7201 atgataattt aaaacgtgaa tcaattttag caaccgcaaa caacttgcgc tgggatgttg
     7261 gagataattt gcatgacaca ttaatggaat ccatttatga aaatgctaca tctatttcca
     7321 gcaaagttgt aacctatccg gataaaaaac ctgcatttag tttagataga actttggata
     7381 aaattcttac cagcaggtat cttggatttc ctataatgtt tttaatactc gctgtggttt
     7441 tctggataac aattgttggc gcaaatgttc cttcgggctt aatagccaat ttacttgtag
     7501 atactttaca tcctgcatta aagactcttg ctaactcaat tggttttccc tggtggttaa
     7561 gcggggtatt aattgatgga gcatatcttg ctatggcctg ggttgtaagt gtaatgcttc
     7621 caccaatggc aatatttttt ccggtataca ctttattaga agattttggt tacttgcccc
     7681 gcgttgcttt taatatggat ccgctttaca aaagagttgg cgcacacggc agacaggcgc
     7741 ttacaatggc gatgggattt ggatgtaatg ctgcaggagt tgtagctaca agagtaatcg
     7801 actctcctcg tgaaagatta attgcaatca ttacaaataa tttttcttta tgcaacggaa
     7861 gatggccaac gcaaattctt attgcaacaa tttttattgg aggcgcagca ccagcatatc
     7921 ttgcaggtat cgcatcagcg ggagcagtag tgtttattgc tgttttaggt attgtattta
     7981 gtttaatcgt ttcctgggga ttatcaaaaa gtttattaaa gggggaagca tccgcattta
     8041 gtttagagct gcctccttat cgccctccaa gaattttgca gacactttat acatctttaa
     8101 ttgatagaac tatatttgtt ttgtggcgtg caattgtctt tgctgtacc
//
LOCUS       DASWBY010000021         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_38677, whole genome shotgun sequence.
ACCESSION   DASWBY010000021 DASWBY010000000
VERSION     DASWBY010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38677"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VF870_00415"
     CDS             <1..850
                     /locus_tag="VF870_00415"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX9250667.1"
                     /translation="AFLAFHDFNNKELLGYGLSGGISFTYFFGLPSDYNIHLKGKLGA
                     SYLTKPFDKEKHSNNMAYSTHLNYLISLGTGITFKLSKQFELQLEGSMNHQSNAALLE
                     PNGGINYWAASVSTNYFLYQPIFTERSIPDPYLTSEKMTRWDLSFSYGISSMPYPMPG
                     QVPMYGITLLRSWQTWRFLAFTLAAELERNGRAVEITRRLNPEKDVDPLRASVLGGVE
                     FLMGRIIFSVQIGVYFYRPFAEQDDIYQRYGLVYNLFRNFYAGINFKSYRNSADHLSL
                     RFTVSF"
     gene            complement(935..1477)
                     /locus_tag="VF870_00420"
     CDS             complement(935..1477)
                     /locus_tag="VF870_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX9250668.1"
                     /translation="MNRLISITTVNDIAAEYRETPLGLLLEYHNLNHDFKNYSEAQLL
                     IGMCMDNRKHLHIPDNFAFIIRSGGANLRYSEFKVSFAISVGGVKHIALIGHSNCGMV
                     NLASNKDIFIQRLSENAGWTVEQAEEHFNNYAPMFEINNEIDFIISETQRLRLRYPKV
                     QIAPMYYKVEDNLLYMIKED"
     gene            complement(1478..2908)
                     /locus_tag="VF870_00425"
     CDS             complement(1478..2908)
                     /locus_tag="VF870_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX9250669.1"
                     /translation="MNKQPHLKRVLGLSDLSFFLIAALVNLNSVPVVASAGPVALVLW
                     LAGFFLFFIPQAIAVLEFTKRAPQEGGIYVWNKKAWGDFHGFVSGWAYWVNNIFYVPT
                     LLFYIIGFILYIGGGTTASLASNPFFMMSVSLGLLWVITYLNIRGFGVGKWIQNLGAS
                     GTFITAATILVIGIITAFTRGSSNDISIAAIIPSLGNWQFLALLSIVCLNYVGLELGS
                     VIADEIKNPVKNIPRAVMVAGASTILLFLVVTSSLLIAIPYKEIGIIEGILQGIEHAA
                     SAIGFGWIVFPMAILMIMNAAGNTSAWLSGAARIPFVIGIDKYLPAALGTIHRKHNTP
                     HVALIVQGIASSFVIVIAAIGSSVGDMYLFLLQTTVVLQLIPYVYMFAALIKVRTTYG
                     SENAYFKNKIITLAAGFIGGGMTILGIAFAFVPAETVSSVFIYEIKLVFSCVAFIIPA
                     LVFYYLNNKRAKLKETAEVAVKKVSI"
BASE COUNT         1041 a          567 c          515 g          935 t
ORIGIN      
        1 cgccttttta gcatttcatg attttaataa caaagaactt cttggttacg gattatcagg
       61 cggaatatcc tttacatatt tttttggtct tccatccgat tataatattc acttaaaagg
      121 taaattaggt gcttcatatc ttacaaagcc atttgataaa gaaaaacact cgaacaatat
      181 ggcctactct actcatctaa attatttaat atcacttgga acgggaataa cctttaaact
      241 aagtaaacag tttgaacttc aacttgaagg aagtatgaat catcaatcca atgcagcgct
      301 gctggaacct aacggcggaa taaattattg ggcagcatct gtttcaacaa attatttttt
      361 ataccaacca atttttactg aacgcagcat tcctgatcca tatttaacgt cagaaaaaat
      421 gacaagatgg gatttgtctt tttcatatgg aatatcatca atgccttatc ctatgccggg
      481 ccaggttccc atgtatggaa taactctctt aagaagctgg caaacctgga ggtttttagc
      541 ttttacactt gcagccgaac ttgaaagaaa cggcagagct gttgaaatta ccagaaggct
      601 taatcccgaa aaagatgttg atcctctccg cgcttctgtt ttaggcgggg tagaattttt
      661 aatgggcaga ataattttta gtgttcagat cggagtatat ttttatcgcc cttttgctga
      721 acaggatgat atatatcaga gatatggatt ggtttataat ttatttagaa atttttatgc
      781 aggtataaac ttcaaatcat accggaattc tgcagatcat ttaagcttaa gatttaccgt
      841 ttcgttttaa ttcaaaagga atttcgttaa ataattttat gcttattaag aatatttagg
      901 gttctgcttg ttctaccgcc ttaatcaata aaatttaatc ctctttaatc atataaagta
      961 aattgtcttc aaccttataa tacatcgggg caatctgtac cttgggatag cgcaggcgta
     1021 atctttgagt ttcgctaata ataaaatcaa tctcgttatt gatttcaaac attggtgcgt
     1081 agttattaaa atgttcttca gcttgttcaa cagtccagcc ggcattttca gaaagtcttt
     1141 gaataaaaat atctttgttt gaagcaagat taaccattcc gcaattgctg tgcccaatta
     1201 aagcaatgtg cttaaccccg ccaacagaaa ttgcaaaaga tactttaaac tcgctgtaac
     1261 gcaaattagc tccaccggaa cgaattataa aagcaaagtt atccggtata tgaagatgtt
     1321 ttctgttatc catacacatt ccaataagca attgtgcttc ggaatagttt ttaaaatcat
     1381 gatttaaatt gtgatattct aaaagcaatc ccagaggcgt ttctctatat tcagcagcga
     1441 tatcattgac tgtagttata gaaataagtc tgttcattta aattgatact ttctttactg
     1501 caacttctgc agtttcttta agttttgctc ttttgttgtt tagatagtaa aaaaccaagg
     1561 ccggaattat aaaagcaaca caactaaaaa ctaatttaat ttcatatata aaaacagaag
     1621 acacggtttc tgccggaaca aatgcaaatg caattcctag tatagtcatt ccaccaccga
     1681 taaatcctgc tgccagggtg ataattttat ttttaaaata agcattttcg cttccgtagg
     1741 ttgtacgcac ctttattaat gctgcaaaca tataaacata aggaataagc tgtaacacaa
     1801 cggtagtctg aagaagaaac aaatacatat caccaacaga tgagccgatt gctgctataa
     1861 caattacaaa gcttgaagca atcccctgaa ctattaatgc tacgtggggg gtgttgtgtt
     1921 tgcgatggat tgtaccaaga gcagcaggca aatatttatc tatcccaatt acaaatggaa
     1981 ttcttgctgc gccggataac caggcagaag tatttcctgc agcattcata atcataagta
     2041 ttgccatcgg aaaaacaatc cagccaaaac ctattgcgct tgcagcatgc tcaatgccct
     2101 gcaaaattcc ttcgataatt ccaatctctt tatatggaat tgcaattaac aaagaggaag
     2161 taacaacaag aaacaataat atcgtagatg ctcctgcaac cattactgca cgcggaatat
     2221 ttttaaccgg attttttatt tcatcggcaa taacggaacc aagttcaaga ccaacatagt
     2281 ttaaacaaac aatgctgagc aatgcaagaa actgccagtt acctagtgag ggaataattg
     2341 cagcaataga aatatcgttt gatgagcctc gtgtaaatgc ggtaataatt ccaataacta
     2401 atattgttgc cgcagtaata aatgttcccg atgccccaag attttgtatc catttgccca
     2461 ctccaaagcc gcggatattt aaatatgtaa taacccagag cagcccgagt gatacactca
     2521 tcataaaaaa tggatttgaa gcaagagatg ctgttgtgcc gcctcctatg tataatataa
     2581 atccgataat gtaaaataaa agagtaggaa cataaaaaat attattaacc caataagccc
     2641 atccggaaac aaatccatga aaatcacccc atgctttttt attccaaaca taaatgccgc
     2701 cttcttgtgg tgctctcttg gtaaactcta aaacagcaat tgcctgtgga atgaagaaaa
     2761 gaaaaaatcc tgcaagccat aacaccagag caacaggacc agcgctcgct acaaccggaa
     2821 cactatttaa attaactaaa gcggcgatta gaaaaaatga aaggtcggat aatccaagta
     2881 cccgtttaag gtgaggttgt ttattcaatt cctgtgtaat tctccggatt ttttttgtaa
     2941 atattattca atcagagttt aataacaata ggaaaaaaat atttatttgt taatcagata
     3001 aagtgagcga gaagaattta taaatgcgta aataatctta aacttaaacc tgaaacat
//
LOCUS       DASWBY010000022         3972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_452970, whole genome shotgun sequence.
ACCESSION   DASWBY010000022 DASWBY010000000
VERSION     DASWBY010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452970"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..382)
                     /locus_tag="VF870_00430"
     tRNA            complement(310..382)
                     /locus_tag="VF870_00430"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(347..349),aa:Thr,seq:cgt)
     gene            complement(589..1296)
                     /locus_tag="VF870_00435"
     CDS             complement(589..1296)
                     /locus_tag="VF870_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9250670.1"
                     /translation="MPKFCNECGYKFEKEYKFCPECGTKVSGSKNNKSKNIESKVSPN
                     VGDKTLNPKVLYGILIGGFAVIAIIILTSGMLDSASVNTEVMNQNQVQTNTNSGVNLN
                     NMQMINDLEAKIKQNPEDYNSLLQLAHLKNDSGLFEAAIQNYKTYLEKNPKDADARVD
                     MGVCYFNLQDYPNAIKEMETALKYAPDHQIAHLNLGVVNLSAGNIEKSKEWLKKAYDI
                     DPTSEVGKKAEQLLNNH"
     gene            complement(1303..1602)
                     /locus_tag="VF870_00440"
     CDS             complement(1303..1602)
                     /locus_tag="VF870_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9250671.1"
                     /translation="MTKADIVDKVAAGTGLTKLETEAIIEGFFSTVIEALKGGHGIEI
                     RGFGSYKVKKKNARNARNPKTGEKVFVDEHYVPTFKFSKEFKDIVNVGMNEQKKG"
     gene            1852..2193
                     /locus_tag="VF870_00445"
     CDS             1852..2193
                     /locus_tag="VF870_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250672.1"
                     /translation="MQLIEIIQLALLIFIALGAVILFFSYIGYRSKSKVKDSSLSKEV
                     EIIEDFPVTKVVEDPDKSTEKVITRKSIKQNPKFEIFKPADDDQTNLDMKKSNQHKLH
                     SPKTLIIKHKS"
     gene            2302..2499
                     /locus_tag="VF870_00450"
     CDS             2302..2499
                     /locus_tag="VF870_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX9250673.1"
                     /translation="MKTVIKIDGMSCNHCVMAVKKEIQKLNVQNLEVKIGEASVEFDE
                     NKINLENIKQAIEEAGYTVIK"
     gene            2509..>3972
                     /locus_tag="VF870_00455"
     CDS             2509..>3972
                     /locus_tag="VF870_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9250674.1"
                     /translation="MKQIATKKIALPVEGMTCASCVARVEKAVAKFDGVTSVNVNLAT
                     EKVSMEYDPSVVDLKKLAETIEDIGYKLDLSALEKSNKSSEEKSGYVDTHLKELKSEL
                     TIAIILSVPIVIINMGMMWKGFFINNFLDFEQINKILLLLTTPVLFVSGKRFYKIFWN
                     NLKHFTADMNSLVAIGTGAAYLYSLILTLFPEILPKNNQNIHVYFDTTAVIITLILLG
                     RWLESRAKSKTGSAIKKLIELQPKTAIVKEQNVEVEKQIDELKLGDIIVIKPGGKIPA
                     DGIITSGSSSVNEAMITGESLPVEKMIGSKVIGGTINTSGYFEFEITMIGKNSVLGQI
                     IKLVEDAQGSKAPIQNLADKVASIFVPVVVLLAILTFIVWLILRPDDLSIALINFVAV
                     LIIACPCALGLATPTALIVGMGKSAQNGILFKYGESLEEIHRADTIVFDKTGTITEGK
                     LSVQDIYFNGISEDEFLSVISSLESKSEHPIARALTDH"
BASE COUNT         1317 a          664 c          661 g         1330 t
ORIGIN      
        1 gatgagtgtc atcccgagcg gagacgaggg acgcagttat cctccctccc actcgccacg
       61 cttcgcctgt cgcagcaggg tagctcccct cctatcttcc acagctaaaa caaaaatacc
      121 gccttcgcaa attatgcgga aggatcacat ttagtgctca tttccaagca cttaaattca
      181 ctttttcttt ttaattaatt gcaactaaaa aactgcccaa acactgccca aaattgtaaa
      241 tactctgatt agtttaggaa aattgggaaa caaaaaagcc ctaaaacatt tgattttagg
      301 gcttttttga gccaactgcc ggaatcgaac cggcgaccta ctcattacga atgagttgct
      361 ctaccagctg agctaagttg gcttatacat tcacaattca agataagaaa caagaaggcg
      421 aagatataac tcttcgcctc ttaataaata agctaaagat aaaaaactat cttactcttt
      481 ttttatgtct gttcttacgt agtctttttt ttcttttgtg tgtagacatt ttatggcgtt
      541 ttcttttttt accacaaggc atcggcgttt tctcctttaa gaaatttact aatgattatt
      601 caataactgt tctgcttttt ttccaacttc gctggttgga tctatatcat aggctttttt
      661 tagccactct tttgattttt ctatattacc tgctgaaaga ttaactacac caagattaag
      721 atgagcaatc tgatggtccg gggcatattt taatgctgtt tccatttctt ttattgcatt
      781 cggataatcc tgcaggttaa aataacaaac tcccatatca actctggcat cagcatcttt
      841 aggatttttc tctaaatatg ttttatagtt ttgtatggct gcttcaaata aacctgaatc
      901 gtttttaaga tgcgctaact gaagcaggct attataatct tccggatttt gtttgatctt
      961 ggcttcaaga tcatttatca tttgcatatt gttaagattt acacccgagt ttgtatttgt
     1021 ctgcacctga ttctgattca taacttcggt attaaccgat gcagaatcaa gcattcccga
     1081 tgttaaaatg ataatggcaa taaccgcaaa tccaccgatt aaaattccat acaatacttt
     1141 aggatttaaa gtcttatccc caacgttagg tgaaactttt gattcgatat tttttgattt
     1201 attatttttt gaacctgata cttttgtacc gcattccgga caaaatttat attctttttc
     1261 aaacttgtat ccgcattcat tacaaaattt tggcatcaac aattaacctt tcttctgttc
     1321 attcattcca acattaacaa tatctttaaa ttcttttgag aatttaaatg taggcacata
     1381 atgttcatca acaaaaactt tttcaccagt tttaggatta cgagcatttc tggcattttt
     1441 ctttttaacc ttataactgc caaatccacg tatttctatt ccatgacctc ctttaagagc
     1501 ttcgattaca gtggaaaaaa aaccttcaat aattgcttcc gtttcaagct ttgtaagccc
     1561 ggtacccgca gcaactttat ctacaatatc cgccttagtc atgtaaaacc ttgataatta
     1621 ataatgtcta tttattttgg ccgggaaaat tatcaaagat ttgtgctaaa atcaacttaa
     1681 ctgcttattt tataatatat tagataaaca accgggagta caagaatggt aaaatcttct
     1741 cattttggat aaaattctct taattgaaat aggaataatc cttttatgat caattttatt
     1801 tatttttaat ggtacgtgta ttgctctgtt aagatcttat aggagagaaa attgcagctt
     1861 attgaaatta tacaattagc acttttgatt tttatcgctt taggagcggt aattttgttt
     1921 ttttcatata ttggatatcg ttcaaaatct aaagtcaaag attcgtcttt atcaaaagaa
     1981 gtagaaataa tagaagattt tcctgtgaca aaagttgttg aggatccgga taagagcaca
     2041 gaaaaagtga taacccgtaa atcgataaaa caaaatccaa agtttgaaat tttcaaacct
     2101 gccgacgatg atcaaacaaa tttagatatg aaaaaatcta atcagcataa attacactcg
     2161 cctaaaactc taataattaa gcacaaatct taacagcctg gttcgcttca tttttaattt
     2221 gtgaccgatg tttcagttaa taagcttatc gttcattata tttaaagaaa taaaaattat
     2281 tcaaataaaa ttataagtaa tatgaaaaca gtaattaaaa ttgatggaat gtcttgcaat
     2341 cattgtgtga tggcagtaaa aaaagaaatt caaaaactta atgttcaaaa tcttgaagtt
     2401 aaaattggcg aagcatcagt tgagttcgat gaaaacaaaa tcaatctgga aaacattaag
     2461 caggcaatag aagaagcagg atatacagtt attaaataat ggtttaatat gaaacagata
     2521 gcaacaaaaa aaattgcatt gccggttgag gggatgacct gtgcaagttg tgttgcgaga
     2581 gttgaaaaag cagtagcaaa atttgatggt gtaacatctg ttaatgtaaa tcttgcaacg
     2641 gaaaaagtgt caatggaata tgatccttct gttgttgatt taaaaaaact tgctgagaca
     2701 attgaagata ttggttataa attagattta tccgcattgg aaaaatcaaa taaatcttct
     2761 gaagaaaaat ccggttacgt tgatacacat ctgaaggaat taaaaagcga actaactatt
     2821 gcaatcattc tttctgttcc aattgttata atcaatatgg gtatgatgtg gaaaggattt
     2881 ttcattaaca atttcctgga ttttgagcag ataaataaaa ttcttttgct gttaacaact
     2941 cctgttctgt tcgtctctgg taaaagattt tacaaaatat tctggaataa tcttaagcat
     3001 tttactgctg atatgaattc acttgttgcg attggaaccg gtgcagctta cttgtatagt
     3061 ttgatactga ctttattccc tgaaatctta cctaaaaata atcaaaacat tcatgtttac
     3121 tttgatacaa cggcagtaat aataacactt atacttttag gaagatggtt ggaaagcaga
     3181 gctaaatcca aaaccgggtc tgcgattaaa aaattgattg aacttcagcc aaagacagct
     3241 attgtaaaag aacaaaatgt ggaagttgaa aagcagattg atgaacttaa gcttggcgat
     3301 attatcgtga taaaaccagg aggtaaaatt cctgcagatg gaattatcac atcaggttca
     3361 tcttctgtca acgaggcaat gattactgga gaaagtttac ccgttgaaaa aatgattgga
     3421 tcaaaagtta ttggcgggac aataaataca tcaggttatt ttgaatttga aataacaatg
     3481 atcggaaaaa attcagtact tggacaaata ataaaattgg ttgaagatgc tcaaggttca
     3541 aaagcaccga tacagaattt agctgataaa gttgcttcga tatttgtacc tgttgttgtg
     3601 cttttagcaa ttttaacatt cattgtttgg ttgatcctaa gaccagatga tttaagtatt
     3661 gcgctaataa attttgttgc tgtgcttatt atagcctgcc cctgcgccct tggattagcc
     3721 acaccgaccg cattgattgt tgggatgggg aaatcagcgc aaaacggtat actatttaaa
     3781 tatggagaaa gtcttgagga gattcatcgt gcagatacaa ttgtattcga taaaaccgga
     3841 actattacgg agggtaaatt atcagttcag gatatctatt ttaatggaat ttctgaagac
     3901 gaatttctat cagtcatatc ttcacttgaa agtaaatcag aacatccaat tgctagggct
     3961 ttaactgatc ac
//
LOCUS       DASWBY010000023         6089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_602484, whole genome shotgun sequence.
ACCESSION   DASWBY010000023 DASWBY010000000
VERSION     DASWBY010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602484"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /gene="gatD"
                     /locus_tag="VF870_00460"
     CDS             <1..828
                     /gene="gatD"
                     /locus_tag="VF870_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HEX9250675.1"
                     /translation="HHTAAALSFMVQNTPVPIVMVGSQRSSDRPSSDAASNLIHAVKT
                     ASESDIAEVMVCMFGPTSDMYGLLHRGTRVRKMHSSYRSTFRTIGDIPIAMVSHEKIT
                     PLREDYKRRRDDKEVIINPVFEEKVSIVYYYPNMQPDIIDSLVDNGYKGIVIAGTGLG
                     HVNKPMYPALKRAREKNVAVYMTVQTLWGYVQMYVYDTGRDMMELGVVPAANMLPEVA
                     YVKLGWALGQTNDLEKVKEIMLTPINGEITEREPSNGYLIYQGGLPEVEEFIKQYRK"
     gene            922..2193
                     /locus_tag="VF870_00465"
     CDS             922..2193
                     /locus_tag="VF870_00465"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9250676.1"
                     /translation="MNRFTILLLLVILFSTNSLPQSPHKYSDIFFLDDHNGWILSTNG
                     YLWKTTNAGTDWIRIYDSRIDTSGKITFVDEQNGWLSLRNTLLRSSNGGLNWVQEYEF
                     PLAFYGYDIEFANDSIGFADDSGRLYKSVDQGQNWELVTDTLGQIYDISFYKDSIGYL
                     CFSHSSPNYITQTRYIYKTTDQGETWIRSYFEGGGLNESVYYDKICIINKTDCIISAF
                     LESPIGGFSSLIKTTDAGSTWELLSDFGVLDLAFVSPQKGWAINGYSIVKTTDGGLLW
                     DTLQTFATWDDVFTNFEFFNPNISYGINQNHIYRTIDGWVTYSIVDSIVTGLNEQQIT
                     ATEFILEQNYPNPFNPNTVIKYQIPITSQVTLKVYDILGNEIATLVNEEKSEGNYSYS
                     FTPSPTLSSGIYFYQLKTDNFIHTKKMLLLK"
     gene            complement(2236..3921)
                     /gene="ettA"
                     /locus_tag="VF870_00470"
     CDS             complement(2236..3921)
                     /gene="ettA"
                     /locus_tag="VF870_00470"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX9250677.1"
                     /translation="MSAEVNKIIYSMVGVSKFYNNKPILKDIYLSYFYGAKIGVIGLN
                     GSGKSSLLKILAGVDKNINGETVISPGFTIGYLEQEPQLDDTKTVREIVQEGVQEVVD
                     LLKEYDEINAKFAEPMDDDKMTKLLERQGEVQEKLEAMGGWDLDARLEMAMDALRCPP
                     SDTLCKVLSGGERRRVALCRLLLQKPDILLLDEPTNHLDAETVAWLEHHLQKYEGTVI
                     AVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKQERLRVEEKRESDRQKTLQR
                     ELEWIRMSPKARHAKSQARITSYENLLKEEKEKGQKDLELYIPVAERLGDIVIETEKL
                     NKAYGDNILIDDLTFSIPPGAVVGIIGPNGAGKTTLFRIITGQEKPDSGNIKIGSTVK
                     MSYVDQSRDALNPDKSIWELISNGDDTIMLGSRQVNSRAYVAQFNFSGADQQKKVGML
                     SGGERNRVHLAMMLKQGANVLLLDEPTNDLDVNTLRALEEGIEKFAGCVLVISHDRWF
                     LDRIATHILAFEGESKVVWFDGNYSEYEEKRKERLGIEADQPHRIKYRHLTRS"
     gene            4252..4755
                     /locus_tag="VF870_00475"
     CDS             4252..4755
                     /locus_tag="VF870_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250678.1"
                     /translation="MLKHSLVLIVFLFSISTLNAQYLGFDDFIKILDLAEERVEAEKF
                     LKLNGFAPYNFEYLTDSEDYEEGDTTSYLIDYYKKLPGDEYFVRVLGYTNDDMYTVME
                     FSSDEDRSFYFVDILSQAGLEPVDEWDDGDGNEGFKFETKKYMMTLEKSPDDDGAMRY
                     QFSISKY"
     gene            complement(4999..5388)
                     /locus_tag="VF870_00480"
     CDS             complement(4999..5388)
                     /locus_tag="VF870_00480"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX9250679.1"
                     /translation="MNKEIKSYNAKQSSEDKKICNMLSAEIDSGLPDAENKVWHAQPV
                     WFLDGNPIVGYSKQKASIRLMFWSGESFEEEKLKPGTGKFKDASIFFTSAEQINFKDL
                     KRWLKKARNIQWDYKNIIKRKGRLVRL"
     gene            complement(5425..6000)
                     /locus_tag="VF870_00485"
     CDS             complement(5425..6000)
                     /locus_tag="VF870_00485"
                     /inference="COORDINATES: protein motif:HMM:NF025432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HEX9250680.1"
                     /translation="MSKTKNTPKELSYKQCEELLNILKSRFEKNINRHKGIEWTKVEA
                     KLKKNTDTLLSLNEMERTGGEPDVVRYDKKTDEYIFYDCSVESPNGRRSLCYDAEALA
                     SRKENKPKDSAVNKAAEMGIELLTEEQYRELQKLGEFDLKTSSWVKTPDNIRKLGGAI
                     FCDRRYDTVFVYHNGADSYYAARGFRGLLKV"
BASE COUNT         1821 a         1134 c         1066 g         2068 t
ORIGIN      
        1 caccatacag cggcagcctt atcctttatg gttcaaaaca ctccggtacc aatagtaatg
       61 gtcggttctc agcgatcaag cgaccgccct tcttctgatg cagcatcaaa tcttattcac
      121 gctgttaaaa cagcatcaga aagtgatatt gccgaagtga tggtttgcat gttcggccca
      181 acatctgata tgtatggact gcttcatcgc ggaacacgag tgcgtaaaat gcattcaagc
      241 tatcgttcta cttttagaac cattggggat attcccattg cgatggttag tcatgaaaaa
      301 atcaccccgc ttcgggaaga ttataaacga agaagagatg ataaagaagt aataataaat
      361 ccggtttttg aagaaaaagt ttcaatcgtc tactactatc caaatatgca gccggatata
      421 attgattcac tggttgataa tggctataaa ggaattgtaa ttgccggtac tggacttggg
      481 catgtgaata aaccaatgta tcctgcatta aaaagagcta gagaaaaaaa tgttgctgtt
      541 tatatgacgg tacagactct ttggggttat gtgcaaatgt acgtttacga tacgggaaga
      601 gatatgatgg aactcggagt tgtacccgca gctaatatgc tgccagaagt tgcttatgta
      661 aaacttggct gggcacttgg acaaacaaat gatttggaaa aagttaaaga gataatgctc
      721 actccaatta atggagaaat aactgaacgt gaaccaagta acggttactt aatttaccaa
      781 ggcggattac cagaagttga ggagtttata aagcaatata gaaagtaggt tcttgactgt
      841 taacattgca tcggtgtttg ttatcttaat tatagcgtct tagaatttat ttagtagcct
      901 aacattttaa cagagaataa aatgaatcgg tttacaattt tattattact tgtaatatta
      961 ttcagcacaa actccttacc acaatctcca cacaaataca gtgatatatt ttttctagac
     1021 gatcacaacg gctggatttt aagcacaaac gggtatttgt ggaaaacaac aaatgcaggt
     1081 acagattgga ttagaattta tgattcgcgg atagacactt caggaaaaat tacgtttgtt
     1141 gatgaacaaa atggctggct tagtttgagg aatactcttc ttcgttcaag taatggtgga
     1201 ttaaattggg tacaagaata tgaatttccc ttagcctttt acgggtatga tattgaattt
     1261 gcaaatgatt caattggttt tgctgacgat agtggtcgtt tatataaaag tgttgatcaa
     1321 gggcagaatt gggaattggt aactgataca ttaggacaaa tatacgatat ctctttttat
     1381 aaagattcaa ttggatattt atgttttagt cattcaagtc cgaattatat aacacaaact
     1441 cgttacattt ataaaacgac tgatcaagga gaaacttgga tccgttctta ttttgaaggg
     1501 ggtggtttaa acgagtctgt gtattatgat aaaatatgta ttattaataa aactgattgc
     1561 ataattagtg cttttttaga aagtccaatt ggaggttttt cctcacttat caaaaccact
     1621 gatgcgggat ctacgtggga attgttatct gattttggag ttttagacct tgcttttgtt
     1681 tcaccccaaa agggatgggc aataaatgga tatagtatcg taaagacaac agatggcggt
     1741 ttgttatggg atactctcca aacatttgct acatgggatg atgtatttac aaacttcgag
     1801 ttttttaatc ccaatatttc gtatggaata aatcagaatc atatttatcg gacaatcgat
     1861 ggatgggtca catattcaat tgtggattcg atagtaactg ggcttaatga acaacaaatc
     1921 acagcaacag aatttatttt agaacaaaat tatccaaatc catttaatcc aaatactgta
     1981 ataaagtatc aaataccaat aactagccag gttactctta aggtttatga tattctggga
     2041 aatgaaattg caactttagt taatgaagaa aaatctgagg ggaattattc ttatagtttt
     2101 actcctagtc caacattatc aagcggcatt tatttttatc aacttaaaac cgataatttt
     2161 attcatacaa aaaaaatgtt gctgctaaaa taatttcacc ctcttagcac tgttgtaaga
     2221 acacaactat tccatttaac tccttgttaa atgccggtac tttattcgat gaggttgatc
     2281 tgcctctatg cccagtctct ctttacgttt ttcttcgtat tcagaatagt ttccgtcaaa
     2341 ccaaacaact ttgctttcgc cttcaaatgc taaaatgtgt gttgctattc tatcaagaaa
     2401 ccatctgtcg tgactaataa ccagaacaca tcctgcaaat ttttcaattc cttcttcaag
     2461 tgcgcgcaat gtgtttacat caagatcgtt tgtcggttca tcaagcaata gaacatttgc
     2521 cccctgcttt aacatcattg caagatgcac gcggtttctt tctccgcctg agagcatacc
     2581 aactttcttc tgctgatctg ccccggaaaa attaaactgt gcaacataag cccgcgagtt
     2641 aacctgtctg ctgccaagca ttattgtatc atcgccatta gaaattaatt cccagatact
     2701 tttatctgga tttaatgcat cccgggattg atccacataa gacatcttta ccgtagaacc
     2761 gattttaata tttccgctgt ccggtttttc ttgtcccgtt atgattctga acaaagttgt
     2821 tttacctgca ccatttggcc ctatgattcc aacaactgct ccgggcggaa ttgaaaaagt
     2881 taaatcatca attaaaatat tatcgccgta tgctttgtta agtttttctg tttcgattac
     2941 aatatcaccc aaacgttctg caactggaat gtaaagctca agatcttttt gtcctttttc
     3001 tttttcttcc ttaagcagat tttcataaga agtaattctt gcctgcgatt ttgcgtgacg
     3061 tgctttggga ctcattctta tccattcaag ttcgcgctgt aaagtttttt gcctgtcgct
     3121 ttcacgtttt tcttcaacac gcaatctttc ctgtttttgt tcaagccacg atgaataatt
     3181 tcctttccac ggaattcctt cgccgcgatc aagctcgagt atccatcccg caacattatc
     3241 aagaaaatat ctgtcgtgtg ttactgcgat aaccgttcct tcatactttt gtaaatgatg
     3301 ttctaaccat gcaactgttt cagcatcaag atgatttgtt ggctcatcga gtaaaagaat
     3361 atccggtttt tgaagaagca atctgcaaag agcaactcgt cttcgctctc cgccagaaag
     3421 aactttacac aaagtgtctg acggcggaca gcgcaaagca tccatcgcca tttcaagacg
     3481 tgcatcaaga tcccatccgc ccattgcttc cagcttttcc tgtacttcac cctgacgttc
     3541 aagaagcttt gtcattttat catcatccat cggttcagca aactttgcat ttatttcgtc
     3601 gtactcttta agaagatcaa caacctcctg aacaccttcc tgcacaattt ctctaactgt
     3661 ttttgtatcg tcaagctgcg gttcctgctc aagataccca attgtgaatc cgggagatat
     3721 aacggtttct ccgtttatat tcttatcaac tccggcaaga atttttaaca gtgatgattt
     3781 acctgatcca tttaaaccga taacaccaat ctttgcacca tagaagtacg ataggtaaat
     3841 atcttttagt ataggtttgt tattatagaa tttgcttaca ccaaccatgg agtaaataat
     3901 tttgtttact tctgcgctca tttaatcttt cttttgataa tgtaatgtgt aaaaataagg
     3961 aaagagaaat taaagttttc aatcagcagt tggcagccgg taattcgcaa agaaagtcga
     4021 ttatttaacg aattggtttg tgggaaccta aatcaggatt tgttgacagg atatgacagt
     4081 tgtaacatac gaaagaattt aagttaaatc attctttgtt attataagaa caaaaactaa
     4141 tttttagtat caattataat tattttttaa ttatgtgctg tttctgcggt gaggatagtc
     4201 ttttaaaaca catcaaaaac tcatttcaat tattttaagg gagaatctac aatgttaaaa
     4261 catagtcttg ttcttattgt gttccttttc agcattagca cattaaatgc tcagtatctt
     4321 ggctttgatg attttattaa aattcttgac cttgcagaag aaagagttga agcagaaaag
     4381 ttcttaaaac taaatggttt tgctccctac aatttcgaat atcttactga ttctgaagat
     4441 tatgaagaag gggataccac cagttacctt atagactatt ataaaaaatt acccggtgat
     4501 gaatatttcg tccgtgttct tggttatacc aacgacgata tgtatacagt aatggaattt
     4561 tccagcgacg aagacagatc gttttatttt gttgatatac tttcgcaggc cggacttgag
     4621 cctgttgatg aatgggatga cggggacggc aatgaaggat ttaaatttga aactaaaaag
     4681 tatatgatga ctctagaaaa aagtcccgat gatgatggcg caatgagata ccaattttct
     4741 atttcgaaat attaattgtt ataaaatatt actatgattt taatgccgct ttttctgcgg
     4801 catttttatt tcagtccttt gctaaattca tcgcacattt ttaaaaagca aacatatttt
     4861 tgattgcttc tttaccccta attctttgat ttgcaacctg atgattaatt gcgtcatttg
     4921 aataaagctc ggacaacaaa tttgcttccc ccttattgaa catctcaacc cattgccgaa
     4981 aaatttcttc tggctaaatt acaacctgac caatctacct tttcgtttta taatgttttt
     5041 ataatcccat tgaatattac gcgctttttt cagccagcgt ttaagatctt taaagttaat
     5101 ctgctcagca gaagtgaaaa atattgaagc gtctttaaat tttccggtac cgggttttaa
     5161 tttttcttct tcaaaacttt caccgctcca aaacataaga cggatgctgg ccttctgttt
     5221 actgtatcca acaatcgggt taccgtctaa aaaccaaaca ggctgagcat gccagacttt
     5281 gttttctgca tcaggcaggc cgctgtcaat ttctgctgag agcatattgc aaatcttttt
     5341 gtcttctgat gattgttttg cgttgtaaga ttttatttct ttattcataa gcccccttaa
     5401 tttattctaa tatggatgtg atctttatac tttcagcaag ccacggaaac ctcttgcagc
     5461 atagtaagaa tcggccccgt tgtgataaac aaaaactgta tcatagcggc gatcgcaaaa
     5521 aattgcgccg ccaagttttc tgatattatc cggagttttt acccagcttg atgttttaag
     5581 gtcgaattca ccaagttttt gaagttcgcg gtattgttct tctgttaaaa gctcaatgcc
     5641 catttcagca gccttattaa cagcgctgtc ttttggttta ttctcttttc ttgatgcaag
     5701 agcttcggca tcgtaacaaa gacttctgcg gccatttggg ctttccacag agcaatcata
     5761 aaaaatgtat tcatcggttt tcttgtcata tctgacaaca tcaggctcac cgccggttct
     5821 ttccatttca ttaagcgaca agagtgtgtc ggtattcttt tttaacttcg cctcaacttt
     5881 agtccattca atacctttat ggcgattaat gtttttttca aatcgtgatt tcaaaatgtt
     5941 aagcagttct tcacattgct tataggataa ttcttttggg gtgtttttag ttttgctcat
     6001 aattttttgc ctcttttaaa taaaaattct taaatcatta attaaaatca agaaaaaatt
     6061 cttttttaag atttcttttt cataataat
//
LOCUS       DASWBY010000024         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_226806, whole genome shotgun sequence.
ACCESSION   DASWBY010000024 DASWBY010000000
VERSION     DASWBY010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226806"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="VF870_00490"
     CDS             complement(<1..1508)
                     /locus_tag="VF870_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX9250681.1"
                     /translation="MNNSRALIVIISMFVFFIALVIKLVNIQIVHAEEYSYYAQRQQT
                     GIEKIAADRGLIYDRNNNLLVYNRSDVSFYVDLRMIKQKHKNEIAEIFSKKFNKSKNY
                     YLNLMKGSKKTVCLEKKAPTEIASDLKNIKRSGLFYREEPTRVFHYNNLASHVLGYLD
                     NENKGVMGISEYYEDALNGEDGSRLIQKNAVGEIVTVDDEEINPAVPGDDIYLTIDKS
                     YQLILEEELRKGISDYGAVSGTGVIMNPNTGEILALANIDDFNPNEYWKYNDFQRRNR
                     AITDTYEPGSTFKSFTIASLIDQKLCRLNEKFNLENGTYKFQNVRIKDSHPMKNLDVV
                     GILEQSSNIGVAKLVQRIDDEKYFKYLRGFGFGNSTSLTLPGETSGRLRKPNEWSKVS
                     KTYLSFGYEISVTPVQIVSGYCALINGGVLYEPQLVQRQISKAGKLVYEFSPKEVRRV
                     ISSETSDTMRDLLHGVVKNGTGKKAYSELISIGGKTGTSQKLVDGTYSKQHY"
     gene            complement(1501..1827)
                     /locus_tag="VF870_00495"
     CDS             complement(1501..1827)
                     /locus_tag="VF870_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250682.1"
                     /translation="MKKSSKPTIFLLITLLLIVTAFALVSIGLKLKYEQFLLQKDKAE
                     KTYKEESQRKIKLTAEYQTVTAEERIVAASTELGLIRNVEPAFIIKYDSNKVEEINEI
                     LKEKYE"
     gene            complement(1824..2747)
                     /gene="rsmH"
                     /locus_tag="VF870_00500"
     CDS             complement(1824..2747)
                     /gene="rsmH"
                     /locus_tag="VF870_00500"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX9250683.1"
                     /translation="MSEHHVPVMLKESLDLLITDRSGIYFDATLGFAGHSSEILKRLN
                     SHGRLIASDVDLDAFEFSRKKFAKESGVSLYNFNYSYIDVIAKIESLQHYDGVLADLG
                     VSSFQLDNPDSGFTYRSDAFLDLRMDKTKKITAADIVNTFTEEDLANVFYQFGEEKNS
                     RKISRTIVEKRSSKKINTTGQLVSIISELVPQNFQRKTLSRIFQALRIYVNDELGNLK
                     LFLENSLTVLKEGGRIVVITYHSLEDRIVKDFFKAETIVNLSPKEDPLGLIKREARLK
                     LITRKPMLPTDEEIRNNYRARSAKLRAAERI"
     gene            complement(2744..>3150)
                     /gene="mraZ"
                     /locus_tag="VF870_00505"
     CDS             complement(2744..>3150)
                     /gene="mraZ"
                     /locus_tag="VF870_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX9250684.1"
                     /translation="KGRIAIPAKLRKHVSADANETFVMTRGLSNCIDLYPLDEWQKIE
                     ESLLNLNSFQPDDARFIRMFVQFAAEDVMDGQSRIMIPSTLITYAKIEKEVLILGALK
                     KIEVWNPKVYEEYLLQSPQTYEEIAAKVMASK"
BASE COUNT          961 a          608 c          451 g         1130 t
ORIGIN      
        1 ttgtaatgct gtttagaata agttccatct actaatttct gagaagtacc tgtcttacct
       61 ccaatagaaa ttaattctga ataagctttt ttgcctgtac catttttaac aacaccatgt
      121 aaaagatctc tcattgtatc agaagtttct gaagaaatta ctcttctaac ttccttaggt
      181 gaaaattcgt ataccaattt tccagccttt gatatttgac gctgcactaa ttgcggttca
      241 tataaaactc cgccattaat taatgcacaa taacccgata caatttgaac tggtgttacg
      301 gaaatttcat atccgaaaga aagatatgtt ttggaaactt tagaccattc attaggcttt
      361 cttagtctgc cagatgtttc accgggaaga gttaatgatg ttgaatttcc aaatccaaaa
      421 cctcttaagt atttaaaata tttttcatca tcaattcttt gaacaagttt tgcaacgcct
      481 atgttgcttg actgttctaa tattccaact acgtccaaat ttttcattgg atgggaatct
      541 tttatcctta cattttgaaa tttataagtt ccgttttcaa gattaaattt ttcatttaat
      601 ctgcataact tctgatcgat aagtgaagca attgtaaatg atttaaaagt tgaaccaggt
      661 tcgtatgtgt ctgttattgc gcggttcctt ctctgaaaat cattatattt ccaatattcg
      721 tttggattaa aatcatcaat gtttgcaagc gcaagaatct ctccggtatt tggattcata
      781 ataacacctg taccggaaac agcaccgtaa tcactaatac cttttcttaa ttcctcttcg
      841 agaatgagct gataactttt atcgatagtt aaataaatat catctcccgg aacagcagga
      901 tttatttcct catcatccac agttacgatt tcaccaacag catttttctg aattagtctg
      961 gaaccatctt ctccgtttaa agcatcttcg taatattcag aaatgcccat cacccctttg
     1021 ttttcattat ccagatatcc aagaacatgc gatgcaagat tattgtaatg aaatactctt
     1081 gttggttctt ccctataaaa tagacctgag cgtttaatat tctttaaatc agaagcaatt
     1141 tccgttggag ctttcttttc caggcaaact gtcttttttg aacctttcat cagattcaaa
     1201 taataatttt tactcttgtt aaacttcttt gaaaatattt ctgcaatttc atttttatgc
     1261 ttttgtttga tcatccttaa atcaacataa aaagaaacat cggatcgatt ataaactaaa
     1321 agattattgt tgcggtcata aatcaatcca cgatctgcag ctattttttc tatgccagtt
     1381 tgttgtctct gtgcataata agaatactcc tctgcgtgca caatctgaat attaaccagc
     1441 ttaatcacca atgcgataaa aaagacaaac attgatatta ttactattag tgcgcgcgag
     1501 ttattcatac ttctctttta gaatctcgtt tatttcttca actttattgc tatcatattt
     1561 tataatgaaa gccggttcaa catttcttat taatccaagt tctgttgacg cagcaacaat
     1621 tctttcttca gcagttacag tctgatattc tgcagtaagt tttatttttc tttgactttc
     1681 ttctttgtaa gttttttccg ctttgtcttt ctgcagaaga aattgttcat atttaagttt
     1741 tagaccgata ctaacaagtg caaacgctgt tacaatcagc aacaaggtta taagcaaaaa
     1801 aattgtgggc ttagaacttt ttttcatatt cgttcggctg cccttagttt tgcacttctt
     1861 gcccgatagt tatttctaat ttcctcatca gtcggaagca ttggttttct ggtaattaat
     1921 tttaatcgtg cctctcgttt aattaatcca agcggatctt cttttggact taagttcact
     1981 atagtttcag ctttaaaaaa atctttcaca attctgtctt caagcgaatg ataagttatc
     2041 acaactatcc gtccgccttc ttttaatacc gtaagtgaat tctcaagaaa aagtttaagg
     2101 ttacccaatt catcatttac ataaattcta agagcctgaa aaattcttga taaagttttt
     2161 cgttgaaaat tttgtggaac cagctcactt atgattgaaa ctaactggcc cgttgtatta
     2221 attttttttg aactgcgttt ttctacaatt gttctggaaa tctttcggga atttttctcc
     2281 tcgccaaact gataaaaaac attcgcaaga tcttcttcag taaaggtatt aacaatatca
     2341 gcggcagtga tctttttagt tttatccatt cgtaaatcaa gaaaggcgtc actacgatat
     2401 gtaaacccgg aatccggatt atctaattga aatgatgata ctcctaaatc agcaagcaca
     2461 ccatcgtagt gttgtaaaga ttctattttt gcaatcacat ctatataaga gtaattaaaa
     2521 ttgtataaac taactccaga ctctttagca aatttttttc ttgaaaattc aaatgcatct
     2581 aaatctacat cagatgctat aagccgacca tgactattta atctttttag aatttcactt
     2641 gaatgcccgg caaatcctaa agttgcatca aagtaaattc cagagcgatc agtaattaaa
     2701 agatccaaac tttctttaag catcacagga acatgatgct cactcattta gatgccatta
     2761 ctttagccgc aatctcctca taagtttgcg gtgattgcag caggtactct tcataaactt
     2821 ttggattcca gacttcaatt tttttaagag caccaagtat caaaacttct ttttctattt
     2881 tggcataggt aattaatgtt gatggaatca taattcttga ttgaccatcc ataacatctt
     2941 ccgcagcaaa ctgcacaaac attctaataa aacgtgcatc atcaggctga aaagaattta
     3001 agtttagaag tgactcttca atcttctgcc attcatccaa aggatacaaa tcgatacaat
     3061 tggacagacc ccttgtcatc acaaaagttt cgtttgcatc tgcagaaaca tgctttctaa
     3121 gtttagccgg aattgcaatc cgacctttag
//
LOCUS       DASWBY010000025         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_453087, whole genome shotgun sequence.
ACCESSION   DASWBY010000025 DASWBY010000000
VERSION     DASWBY010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453087"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1289
                     /locus_tag="VF870_00510"
     CDS             129..1289
                     /locus_tag="VF870_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9250685.1"
                     /translation="MKVLKLIFKNSLRHKLRTSLTILGIAIAVIAFVLLRTVVTAWYA
                     GVEASAADRLIVRQAVSFIFPLPYAYKEKIENISGVQTVSYANWFQGVYIDKDQFFAR
                     LAVDAETFFDVYPEFVLTKQELAKFKAERNGCVVGEDIAKKYNLKLGDVMTIDGDIYP
                     GQWEFVIRGIYKPRDKTTDATQMVFHWKYLDERMREESPIRGGDVGWYIIKIKDPNQS
                     GSISEQIDALFKNSRAETKTETERAFQQGFVQSTSAIITSMNVLSFVIIGIIMLVLGN
                     TMIMSARERTREYAVFKTIGFSGNHIIGLIMGESFLISAFGAAVGLILTFPIVASFQK
                     FIPKGFFPVFEIETITIVFAVLSALLIGIAASIFPIQRALKTSIVEGFRFVG"
     gene            1293..2462
                     /locus_tag="VF870_00515"
     CDS             1293..2462
                     /locus_tag="VF870_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9250686.1"
                     /translation="MKIPIKYTIKNFSSRKLTTAITLVGISLVVFVFAAVLMMAYGIQ
                     KTLIATGSTDNVMVVRKSANGEISSLVDGDTQNVIRTLPHIKTAADGSQIISNEPVVI
                     INLARKGGGVSNVTVRGVSPSVQLLRPQVKIVEGRMFTFGLKELIAGRSIKERFKGAS
                     IGETVNFAGDKWTVIGIFDTDGSGFDSEMWGDSQQLLSSFNRGNTVSSVTLKLDNVSN
                     FESFKRAFEVDRRLQQYEAKIEKKFFEEQSELMATFIRILGIFITIIFSFGAIIGAMI
                     TMYSSVANRTVEIGTLRALGFKRRSILSAFMAESLLVSISGWIIGILLASTLSFFKIS
                     TLNLGSFSELEFSFALSPSIVISSLIFAVLMGVLGGFLPSVRAARLNIVKALRAG"
BASE COUNT          816 a          382 c          518 g          835 t
ORIGIN      
        1 acactgatgg aaagattgaa taaagaattt caaaagacca taataatggt aactcacgat
       61 ccgcacgctg ctgaaagggc ttcccataca cggcatcttg ataaaggcga tttggtttag
      121 gtacaattat gaaagtatta aaattaattt ttaagaattc attaagacac aagcttagaa
      181 catctctaac aattcttgga atagctattg ctgtaattgc atttgtgctt ttaagaacgg
      241 tagttacagc atggtatgca ggtgttgaag cttcagctgc cgatcgactt attgttaggc
      301 aagcggtctc ttttattttt ccattgccct atgcctataa agaaaaaata gaaaacattt
      361 caggagtgca gactgtttct tacgcaaact ggtttcaggg tgtgtatata gataaagatc
      421 aattttttgc cagacttgct gtagatgcag aaactttttt tgatgtttac cctgaattcg
      481 ttcttactaa acaagaacta gcgaagttca aagccgaaag gaatggttgt gttgttggtg
      541 aagacattgc aaaaaaatat aatttaaaat taggcgatgt tatgactatt gacggtgata
      601 tatatcctgg ccaatgggaa tttgttattc gtggaatata taagccaaga gataaaacaa
      661 cagatgcaac tcaaatggtc tttcattgga agtatctgga tgagcgtatg agggaagaat
      721 caccaatccg tggtggtgat gttggctggt acatcattaa aataaaggat ccaaatcaat
      781 caggctcaat ttcagaacaa attgatgcgc ttttcaagaa ttctagagcg gaaactaaaa
      841 ctgagaccga gcgggctttt cagcagggat ttgttcaatc aacaagtgct atcattactt
      901 caatgaatgt tctttcattt gtgataattg gtattataat gctggtgctt ggaaatacga
      961 tgataatgtc agcaagagaa aggaccagag aatatgctgt attcaaaacc attggttttt
     1021 ctggtaatca cataataggt ttgataatgg gtgaatcatt tttaatttca gcttttggtg
     1081 cggcagtagg attaatttta acatttccaa ttgtggccag ttttcaaaaa tttattccca
     1141 aaggattctt ccctgtattt gaaatagaga caataacaat agtatttgct gtactttctg
     1201 ctttattaat cggaatagca gcttcaatat ttccgattca aagagcatta aaaacctcca
     1261 ttgtagaggg attcagattt gtaggataat ttatgaaaat acccattaaa tacactatca
     1321 aaaattttag ttcaagaaag cttaccacag caattacttt ggtcggtatt tcacttgtcg
     1381 tttttgtgtt tgccgctgta ttaatgatgg cctatggaat tcagaaaaca ttaattgcaa
     1441 ccggatcaac tgataatgta atggtggtgc gcaaatctgc aaatggtgaa atttcaagtc
     1501 tagtcgatgg cgatactcaa aatgtaatca gaacattacc gcatattaaa actgcagctg
     1561 atggaagtca gattatttct aacgaacctg ttgtaattat aaatcttgca aggaagggag
     1621 gaggagttag taatgttaca gtgcgtggtg tttctccctc tgtgcaacta ttaaggcctc
     1681 aagttaaaat agttgaagga aggatgttta cttttggttt aaaagaatta atcgctggaa
     1741 gatctattaa agaaagattt aaaggtgcaa gcataggcga aacagttaat tttgccggcg
     1801 ataaatggac ggttattgga atatttgata ctgatggtag tggttttgat tctgaaatgt
     1861 ggggcgattc tcagcaactg cttagttcat ttaatagagg aaatactgtt tcttcagtta
     1921 cattgaaatt agataatgtt tcaaactttg aaagcttcaa aagagctttt gaagtggatc
     1981 gaagacttca gcaatatgaa gcaaaaattg aaaagaaatt ttttgaagag caatctgaat
     2041 tgatggcaac atttattcga atacttggaa tctttatcac aattattttt agttttggcg
     2101 ctatcattgg tgcaatgata acaatgtatt catcagttgc aaacaggact gtagaaattg
     2161 gaaccttacg tgcgcttggg tttaaacgaa gaagcatact ttcagctttt atggcagagt
     2221 cacttttagt ttcaatctcc gggtggataa ttgggatcct tctggcgtca acattatcat
     2281 tctttaagat ttctacacta aaccttggct cattctctga actagaattc tcttttgcac
     2341 tctcaccatc gatagtaata tcatctctaa tttttgcagt tttgatggga gtgttaggcg
     2401 gatttttacc atcggttcgt gcagcaagat taaatattgt taaagcttta agagctggtt
     2461 aagaataatg tttgttattc ccgcggaagc aggaatttac ttaatgatta gagaaaggta
     2521 tattaatttt ttatttattc aaaagaatat c
//
LOCUS       DASWBY010000026         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_453183, whole genome shotgun sequence.
ACCESSION   DASWBY010000026 DASWBY010000000
VERSION     DASWBY010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453183"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF870_00520"
     CDS             complement(<1..189)
                     /locus_tag="VF870_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250687.1"
                     /translation="MKPAIIFFSTIFFVLAGLIFIESGFIATDPVSITPINNTDVIKD
                     KISNSKFTGLEFSILNSTD"
     gene            complement(216..1172)
                     /locus_tag="VF870_00525"
     CDS             complement(216..1172)
                     /locus_tag="VF870_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250688.1"
                     /translation="MNDIEKIIELLDKDSITSDEKKILDGITEQNPEAKKISQIYLKL
                     KMSLKQNDHIDEELMGEYVLYKNNNSSERVIILLSKMIEDHIRKCNQCENLFKELNAE
                     YTEVDEFIIKSVSDQIIQNKQSKNKTFSLFNNFGAVKYAFASLTVFIALYLGLFMISS
                     FTTPDYKKAFIEDEDFYTTRGRTSELFQRGLDAIDKKDFESAIQYLNDDINKNPNDES
                     IFYTHFVLGITYVNKAETNFLGLFKSFDRAEVQKGIVNFEESIELNKSGRFDNLNLDA
                     QYFIGKSYLMIDDKASAKEHLQLVVDGKGSYYKKAEDLIKYL"
     gene            complement(1235..1777)
                     /locus_tag="VF870_00530"
     CDS             complement(1235..1777)
                     /locus_tag="VF870_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9250689.1"
                     /translation="MDFLNFSKTELESLLISSKEGNSEAFNKISRYVREISYSYFLSK
                     YRAHKIVNKDDVDDLATNVYISFAEQYHKIDIIENWLRRVLFLTFVRWYKQNSARKTV
                     ELNEEITADDSNLKTDEGFDVNAILKVLDSLSEEKREIVRLRFWGDLKFAEIAEKLNK
                     NEAAIKKMFYRTIEEIKNKL"
BASE COUNT          639 a          321 c          270 g          747 t
ORIGIN      
        1 atctgtactg tttaatatag aaaattccag ccctgtaaac ttagaatttg aaattttgtc
       61 ttttattaca tcagtattgt ttattggagt aattgaaacg gggtcagttg caataaaacc
      121 agattcaata aaaatcaaac cagccagaac aaaaaatatc gtagaaaaaa atataattgc
      181 aggtttcatc atttttattt gtcatccgaa caatattata aatatttaat aaggtcttct
      241 gcttttttgt aataactgcc tttaccatca acaactaatt gaagatgctc ttttgcagat
      301 gctttatcat cgatcattaa ataagacttg ccaataaaat attgcgcatc aagattaaga
      361 ttatcaaacc tgccgctctt attaagctcg attgattcct caaaattcac aattcctttt
      421 tgaacttcgg ccctatcgaa tgacttaaag agaccaagga aattagtttc tgctttgttt
      481 acatatgtaa tgcccaaaac aaagtgagta tagaaaattg attcatcatt cggattttta
      541 ttaatatcgt cattaagata ttgaattgct gactcgaagt ctttcttatc aattgcatct
      601 aatccgcgct ggaataattc agaggttctg cccctggtgg tgtaaaaatc ttcatcttct
      661 ataaaagctt ttttgtaatc gggagttgta aatgaagaaa tcataaagag acctaaatat
      721 agagcaataa atacagttag agatgcaaat gcatatttta ccgcgccaaa attgttaaat
      781 aaagagaatg ttttattctt agattgtttg ttttgtataa tttggtcgga aaccgattta
      841 atgataaact catcaacctc tgtgtattca gcatttaatt ctttaaataa attttcgcac
      901 tgattacatt tacgaatatg gtcctcaatc atctttgaaa gaaggattat tactctttcc
      961 gatgaattgt tatttttata caatacgtat tcgcccatta gctcttcatc aatatgatcg
     1021 ttttgcttga gcgacatttt taattttaaa tatatctgac ttattttttt tgcttcagga
     1081 ttctgttcag taattccatc aagaattttt ttttcatcgc tagtaattga atccttgtcc
     1141 aataattcaa ttattttttc aatatcattc atttataaat acctttttgt gcttcttaac
     1201 cattagtaat gacaatcatt taaaagtgac attattataa tttatttttt atttcttcaa
     1261 tagtacggta gaacattttc ttaattgctg cttcattttt attcagcttt tctgcaattt
     1321 cagcgaactt caaatcaccc cagaatctta accttactat ttcacgtttt tcttcactta
     1381 agctatctaa tacttttaag attgcattta catcaaaacc ttcatcagtc ttgaggtttg
     1441 aatcatctgc agtaatttcc tcgttaagtt caacagtttt cctagcacta ttttgcttat
     1501 accatctaac aaaagtcaaa aaaagtactc tgcgaagcca gttctcgata atgtcaattt
     1561 tatgatattg ttcggcaaaa gatatatata catttgtagc taagtcatca acatcatcct
     1621 tgtttacaat tttgtgtgcg cggtatttcg ataaaaaata ggaataggat atttccctga
     1681 cataacggga tattttattg aatgcttcag aattcccttc ttttgaagaa attagaagag
     1741 attcaagctc agttttggaa aaatttagaa agtcgatttt tgtctctctt tatattgtca
     1801 ctatttttat attattagta ctaagataaa taaactcgtg actatgaata aatattttat
     1861 tacaatttta tttttgataa cactggcagg cagccttttt gcccagccaa atataagaat
     1921 cgaacctaga aatattcgat ttgtagatgt ttttaacaga tatgattatg cgataat
//
LOCUS       DASWBY010000027         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_715703, whole genome shotgun sequence.
ACCESSION   DASWBY010000027 DASWBY010000000
VERSION     DASWBY010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715703"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1691
                     /locus_tag="VF870_00535"
     CDS             <1..>1691
                     /locus_tag="VF870_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250690.1"
                     /translation="GIIRGTNEPLVTTASIDAASISATRGGGFEYTNGVGEKVIERSS
                     LFDSPFYQPSAISHQDFIMDYTDTNTVYLNGEPITDHHPLGIVVHQESYAWNFSFADF
                     FVIMNYTIRNVTNKYIDSVFVGLWTDTVVRNTKITGRPSGSAFYSKGGNGYNDSIKIA
                     YEFDATGDPGYTDSYIGIQYLGSEPKLPDKILMDGDSVNSVSFVSWTHNGSDPSFFTP
                     SDDNQRFNKMRGYFDLGNSQRWKDPISAATLKTPSNRSMLISNGNFSSIAPGDSINVV
                     FAIVCAKKYGDDPAYMDTDEQKTNLYINADWALRAYFGEDRNRNGVLDPGEDLDGNGE
                     ITRYILPTPPTSPKVKVVPDNQKATIYWDMRAENSIDPISGKKDFEGYKLYRTQAGFD
                     LSQTQNLQNSLVVLAEFDSTGNSVGFNTGFSAVRLDEPVTFPNDTTKYFYKFELPNLL
                     NGWQYVFTVTAFDEGDPENELDILESSSISNYERIVPGTPAVEDENVEIGVYPNPYYG
                     SAVWDGNSERLRKIYFYNLPAECEITVYTLAGDIVKKLTHDAASNGADTRWFENYA"
BASE COUNT          544 a          268 c          337 g          542 t
ORIGIN      
        1 ggaattatta gagggactaa tgaacctttg gttactactg cttcaattga tgctgcttcg
       61 atttctgcta caagaggcgg aggttttgaa tatactaatg gtgttggtga aaaagtaatt
      121 gaacgctctt ctttatttga ctctccattt tatcagccat ctgcaatttc tcatcaagat
      181 tttataatgg attatacaga tacaaatact gtttatttaa atggcgaacc tataacagat
      241 catcatcctc ttggtattgt tgtgcatcag gaatcttatg cctggaattt ttcatttgca
      301 gatttttttg tgattatgaa ttatacaatt aggaatgtca caaataaata tatcgatagc
      361 gtttttgtgg gactttggac agatacagtt gttagaaaca ctaaaattac cggaagaccc
      421 agcggatcag cattctattc aaagggtgga aatggttaca acgattcaat aaaaattgcg
      481 tatgaatttg atgctactgg cgaccccgga tatactgata gttatatcgg gattcaatat
      541 cttggttcag aaccaaaatt accagataaa attttaatgg atggtgattc agttaattct
      601 gtaagttttg ttagttggac tcacaatggt tcagatccat cattctttac tccatctgat
      661 gataaccaaa gattcaataa aatgcgcgga tattttgatt taggtaattc gcaaagatgg
      721 aaagatccga ttagtgcagc tacgcttaaa acaccttcaa acagatctat gttaattagt
      781 aatggtaatt tttcatcgat cgcgccagga gattccatta atgttgtatt tgcaattgta
      841 tgtgcaaaaa aatatggaga tgatcctgcg tacatggata cggatgaaca aaaaacaaat
      901 ctttatataa atgctgattg ggctttgcgt gcatactttg gtgaagatag aaatagaaat
      961 ggtgttctcg atccgggtga agatttggat ggtaatgggg agattacacg atatatactt
     1021 ccaactccgc caacatcgcc aaaagtaaaa gtagtacctg ataatcaaaa agccactatt
     1081 tattgggata tgagggccga gaattctata gatcctattt ccgggaaaaa agattttgaa
     1141 ggttataaac tttacagaac acaggctgga tttgatttat cacaaacaca aaatttacaa
     1201 aactctttag tagtacttgc tgagtttgat agtaccggaa attctgtcgg atttaataca
     1261 ggcttttccg cagttcgatt agatgaaccg gttacatttc caaacgatac aactaaatat
     1321 ttttacaaat ttgaactacc aaaccttctg aatggctggc aatatgtttt tacagtaacc
     1381 gcgtttgatg aaggtgatcc tgaaaatgaa ctcgatattt tagaatcgag ttccatttca
     1441 aactatgaaa gaattgtgcc cggaacgcct gcagttgaag atgagaatgt tgaaattggt
     1501 gtctatccaa atccatacta tggaagtgca gtttgggatg gaaattctga aagattacgt
     1561 aaaatttatt tttataacct tcctgcagag tgtgaaatta cggtttatac gcttgccggt
     1621 gatatagtta aaaagttgac tcacgatgct gcaagcaatg gtgccgatac acgatggttt
     1681 gaaaattatg c
//
LOCUS       DASWBY010000028         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_38896, whole genome shotgun sequence.
ACCESSION   DASWBY010000028 DASWBY010000000
VERSION     DASWBY010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38896"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..1190
                     /locus_tag="VF870_00540"
     CDS             357..1190
                     /locus_tag="VF870_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9250691.1"
                     /translation="MNKDFRLKTNDGDFINFSSFGLENIKSAPCLILVHGFKGFKDWG
                     FFPYTAKYFADKGFFVLSFNFSHNGIDDQGSYFNRLDKFAKNTVSLEVSELVQVIAAY
                     NNGFFEENVFGKVCLIGHSRGGGVAILSSLVEKVDAYIVWASVERFDRYTDRQKAEWR
                     KQGFVEVLNSRTNQMMRMNVELLEDVETNKNGSLNIEKAVKSLGKPLMIIHGTEDLTV
                     PIAEGEQIYNWSDKSITQFEKLAACGHTFDIVHPFEGSNKKFDLVLSKTEEFLRKVFK
                     I"
     gene            1339..>2875
                     /locus_tag="VF870_00545"
     CDS             1339..>2875
                     /locus_tag="VF870_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX9250692.1"
                     /translation="MELKNIIFIIVFLTVFGFFAYSVNNLINYLKVAKKKDDRFDNIP
                     TRLKRVWNIAFAQTKLLRDPVAGTLHFLIFWGFVLFIFAVIETIFQGFYSKFSLSSLG
                     PIYTLITLVQDLFGLLVILSIIISLYRRFVKKVPRLIVDKHGMMDAAFILSLIMFIVL
                     SMYGQNAAGYANNGMAYHESELRPVSAFISGLLFTGQTSSTIFMYELFWWMHILLIFG
                     FLNYLPYSKHLHVLSSIPNVFFANLDPVRNTIKKLNLDDENAETFGAADIDQFSWKQI
                     LDGYSCTECGRCTSVCPANTVGKSLSPRDIIVDIRKRTLDKAPLIVEGKSEGELFEKT
                     LVHNYITDTALWECTTCMACVQECPVMIEHVDSIVDMRRNLVLTESEFPANLNPVFKS
                     LETNFTPWAFNPADRAEWAEGMNIKSMAEDKDGEILFWVGCAGSFDDRYKKVSKAFAT
                     IMQKAGVDFRILGTEEKCNGDTARRLGNEYLAQMMMQENVETLNNYGVKKIVTACPHC
                     FHSL"
BASE COUNT          947 a          380 c          551 g          997 t
ORIGIN      
        1 ataactaaat gaagtgagaa agtaaaagaa caacgactgt tataattttt gcagatcata
       61 tttccaaacc tgaattatat taaagaattt ctataatctt caaattctca aaactacagt
      121 tgccccgaac aattgaaatt gaagagcatt atttagttgt tagctttaga tttatcttag
      181 gttttagatg tgttgtacgt tacgtaaata gtattattgt gagaatagtt ggagcagaat
      241 taaataaaaa catcgaaaac agaataaaaa ccaagtatca cgtttgccaa gataatattg
      301 cctcaaaatc tttaaaaata ttgacttttc taacttaatt gttcatattt caaacaatga
      361 acaaagattt taggctaaaa acgaatgatg gggattttat aaacttcagt tcatttggat
      421 tagaaaatat aaaatcagca ccttgcttaa ttttagttca tggttttaag ggttttaagg
      481 attggggttt ctttccatac actgcaaagt attttgcaga taaaggtttt ttcgttctat
      541 cttttaattt ttcgcataat ggaattgatg atcaaggatc atattttaat cgtttagata
      601 aatttgcaaa aaacacagtt tcactcgaag tttcggaact cgttcaagta atagctgctt
      661 acaataatgg attttttgaa gaaaatgttt ttggtaaggt ttgtttgatt ggtcatagtc
      721 gtggtggtgg ggtagcgatt ttaagtagtt tagttgaaaa ggttgatgca tatattgttt
      781 gggcgtcagt tgaaagattt gatagatata ctgataggca aaaagctgaa tggcgaaaac
      841 aaggatttgt tgaggtttta aactcaagaa caaatcaaat gatgcgtatg aatgttgaat
      901 tgttagaaga tgttgagaca aacaaaaatg gttcattaaa tattgaaaaa gcagttaaaa
      961 gtttaggcaa acctttaatg atcattcacg gtaccgaaga tttaactgtt cccattgcgg
     1021 aaggtgagca gatttataac tggagtgata aatcaattac acaatttgaa aaacttgcag
     1081 cttgtggtca tacatttgat atcgtgcatc cgtttgaagg aagtaataaa aagtttgatt
     1141 tggtgctttc aaagacggaa gagtttctaa gaaaagtgtt taaaatttga gctaagtata
     1201 ttttgtcatt tcgaaggagt cttcgactga gaaatctcca accattaaac ggacagattt
     1261 ctcgctttgg tcgaaatgac aattaaattc gttttactat aaagataaaa gtacataaag
     1321 tttattaaat agttttatat ggaattaaag aacatcattt ttatcatagt ttttttaacg
     1381 gtatttggat tttttgcata cagtgtaaat aatctgatta actaccttaa agttgctaaa
     1441 aagaaagatg atagatttga taatattcca accagattaa aacgtgtttg gaacattgca
     1501 tttgcccaaa ctaaactatt acgtgatcca gttgcgggga ctttacattt tttaattttc
     1561 tggggatttg ttctttttat ttttgcagtg attgaaacaa tctttcaagg gttttattca
     1621 aaattttcac tttcgtccct tggtcctatt tacacactga ttactttggt gcaggattta
     1681 tttggattgc tggttattct atcaataatt atttcgcttt acagaagatt tgttaagaag
     1741 gtaccaagat taattgttga taaacatggc atgatggatg cggcatttat tctttcttta
     1801 ataatgttta ttgttctttc gatgtacgga caaaatgcag ccggatatgc taataatgga
     1861 atggcatatc atgaatcgga gttaagacct gtttcagctt ttataagtgg actattattt
     1921 acaggacaaa cctcatcaac aatttttatg tatgagcttt tttggtggat gcacatttta
     1981 ttgatttttg gttttctgaa ttatttgccg tattcaaagc atcttcatgt tttaagttca
     2041 attccaaatg tgttctttgc aaatctggat cctgttagaa atacaattaa gaaacttaat
     2101 cttgatgatg aaaatgctga aacattcggc gctgcggata ttgatcaatt tagctggaag
     2161 caaattttag atggttattc ctgcacagaa tgcggaagat gtacatctgt ttgccctgca
     2221 aatactgttg gtaaatcatt atcaccaaga gatataattg tagatattcg aaagcgaact
     2281 ttagataaag ctcctttgat agttgaaggg aaatctgagg gtgaactatt tgagaaaact
     2341 cttgttcaca actatataac agatactgct ttgtgggaat gcacaacctg tatggcttgt
     2401 gttcaggaat gtcctgttat gattgaacat gtagattcga ttgttgatat gcgcagaaat
     2461 ttagttctga ctgaatcaga atttccggca aacttaaatc ctgtgttcaa gagtttggaa
     2521 actaacttta ctccctgggc ttttaatcct gcagacagag cagaatgggc agaaggaatg
     2581 aatatcaaat ctatggcaga agataaagat ggtgagatat tattttgggt tggctgcgca
     2641 ggatcttttg acgatagata caaaaaagtt tctaaagctt ttgctactat aatgcagaaa
     2701 gcaggagttg attttagaat tcttggtacc gaagaaaaat gtaacggcga tactgcacga
     2761 agactgggta atgaatatct tgctcaaatg atgatgcagg aaaacgttga aacattaaat
     2821 aattatggtg ttaaaaaaat tgttactgct tgtccgcatt gctttcattc attaa
//
LOCUS       DASWBY010000029         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_264404, whole genome shotgun sequence.
ACCESSION   DASWBY010000029 DASWBY010000000
VERSION     DASWBY010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264404"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VF870_00550"
     CDS             complement(<1..269)
                     /locus_tag="VF870_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9250693.1"
                     /translation="METTINKVLVPIDFSDYSKSALKYAVNFAKSFNADIILIYVVEP
                     IIYPPDFSMGQIAMPSINTEWDDRAKEELQKLAKNEITGVKNVKT"
     gene            complement(253..810)
                     /locus_tag="VF870_00555"
     CDS             complement(253..810)
                     /locus_tag="VF870_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9250694.1"
                     /translation="MKNEKVVHSSFWVNFFNSPTEETDLKNSLKTIPLFNELNKSDLS
                     SLIKIIHNRTFVSGELIFHQGDPGIGLYIIREGEVEIERENDHQVKKILATFSKGDFF
                     GELALVDGEKRSASAIAKTACKLSVIFKPDLDEFIEKYPRKGIKILTGISSMIALRLR
                     KLNEDYFDLQYNIPEEKGKQDGNND"
     gene            complement(819..1583)
                     /locus_tag="VF870_00560"
     CDS             complement(819..1583)
                     /locus_tag="VF870_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEX9250695.1"
                     /translation="MNPIKKHLLVIPSIDIQNRKTVRVVQGIPELDCKEYGDDPVEMA
                     MIWRAENAKMLHVVDFNGVFDHTKENYGIIKNLCSSVIIPIEFAGGIRSIEDAEAVFD
                     LGVHRIAINTMALENRAEFIKLFEKYGPSKIVLSIDIINGELVTRGRKKTSGLNYLSF
                     TKEMVDLGIDRCIVTDVSRNGVMQGPNIALSKEIAEHSKIKVTHSGGLRNKDELLDLQ
                     MLIPIGVDSVIIGRAFYENRFPCQKLWRVAESGLFN"
BASE COUNT          690 a          365 c          289 g          740 t
ORIGIN      
        1 attgttttaa catttttaac tcctgtgatt tcatttttgg caagtttttg caactcttct
       61 ttagctctgt catcccactc agtatttatg gaaggcattg caatttgtcc catactaaaa
      121 tcaggcggat aaataatagg ctcaacaaca taaatcaaaa tgatatctgc attaaaagat
      181 tttgcaaaat taaccgcata ctttaatgcg cttttagaat aatcagaaaa atcaatcggc
      241 acaagaacct tattaatcgt tgtttccatc ttgttttcct ttctcttctg gtatattgta
      301 ttgaagatca aaataatcct catttaattt tctcaatctt aaagcaatca tgctggaaat
      361 cccagttaat attttaattc cttttcttgg atatttttcg ataaactcat caagatcagg
      421 tttaaagata actgaaagct tacaagcggt ttttgcaatt gctgaagctg atcttttttc
      481 gccatcgact aaagctaatt caccaaaaaa atcgccttta gaaaaggtag ctaaaatctt
      541 tttaacttga tgatcatttt ctctttcaat ttcaacttca ccttctcgaa ttatgtacaa
      601 tccaattccg ggatcgcctt ggtgaaatat taattctccc gaaacaaaag tgcggttatg
      661 aataatctta atcaaagatg atagatcact tttatttaat tcattaaaaa gcggaattgt
      721 ctttaatgaa tttttcaaat ctgtttcttc agtaggtgag ttgaaaaaat ttacccaaaa
      781 actactgtgt actacttttt catttttcat aatatccttt aattaaataa acctgattca
      841 gcaacgcgcc aaagtttttg gcagggaaat ctgttttcgt aaaatgctct gccgatgatt
      901 acagaatcaa ctccaatagg aattaacatt tgtaaatcta aaagttcgtc tttatttctt
      961 agtccgccgg aatgagtaac tttaatttta ctatgctctg caatctcttt tgaaagtgca
     1021 atgtttggtc cctgcataac accgttacga ctaacatctg taacaatgca tctgtcaatt
     1081 ccaagatcaa ccatttcttt agtaaatgat aaataattta atcctgatgt cttttttctt
     1141 ccacgagtaa ctaattcacc attaattata tcaatggaga gcacaatttt agaaggccca
     1201 tatttttcaa acagttttat aaactctgca cgattctcca aagccattgt atttattgca
     1261 attctgtgta cacccagatc aaaaactgct tctgcatctt caatgctgcg tattcctcct
     1321 gcaaattcta tcggaatgat tacagaactg caaaggtttt taattatgcc ataattttct
     1381 ttggtatgat cgaatacgcc attaaaatct acaacgtgaa gcatctttgc attttctgca
     1441 cgccatatca ttgccatttc aacaggatcg tcgccgtatt ctttacaatc gagttccgga
     1501 attccctgaa caactctaac agttttgcgg ttttgaatat caattgaagg aattactaaa
     1561 agatgttttt ttataggatt catagcgtta agaaataatg atgtttattc ctgttaaaaa
     1621 ataactaaat gaagtgagaa agtaaaagaa gaacaactgt tataattttt gcagatcgta
     1681 tttccaaacc tgaattatat taaattattt atataatctt caaattctca aaactacagt
     1741 tgccccgaac aattgaaatt gaagagcatt atttagttgt tagctttaga tttatcctag
     1801 tttttagatg tgttgtaagt tacataaata gtattattgt gagaatagtt ggagcagaat
     1861 tcaataaaaa catcgaaaac agcatataaa ccaagcatca cgtttgccaa gattatattt
     1921 cctcaaaatc tttaaaaata ttgacttttc taacttaatt gttcatattt caaacaatga
     1981 acaaagattt taggctaaaa acgagtgaag gggattatat aaacatcaat tcatttggat
     2041 tagaaaatat aaaatcctca ccttgcttaa ttttagttca tggt
//
LOCUS       DASWBY010000030         3271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_377310, whole genome shotgun sequence.
ACCESSION   DASWBY010000030 DASWBY010000000
VERSION     DASWBY010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377310"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VF870_00565"
     CDS             complement(<1..480)
                     /locus_tag="VF870_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250696.1"
                     /translation="MLNKTLFILITFLIADIGFSQVSTQTQKYRDEPKGNIDFRKEGI
                     MDGNQIRTLFYNNGEVGHWPDQPSGEWPKGTGYSYLDGVAVLIASEITAPGNNQIVHP
                     LQTSYREWMDKDPSTGEIWGLEPVPGYSNPNQVNEYPNERNSPAINTNPHTWPDKWPA
                     "
     gene            complement(502..>3271)
                     /locus_tag="VF870_00570"
     CDS             complement(502..>3271)
                     /locus_tag="VF870_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9250697.1"
                     /translation="ILRFFIPLVVLLLTVPLFAGTTGKISGTVTDQETGEPIVGANII
                     IEGTYLGASADLEGYYSISNIPPGEYRVIFSAVGYQKIIVEKVRVNIDLTTNLDMKLS
                     SSVIQLNQEVVVTSQRPLVQKDLTSTSVTISSNEIKMMPVESIGQIVNLQAGVIDGHF
                     RGGRSNDVAYLIDGVAVTDAFNGGFGVQVENSSVRQMEVISGTFNAEYGQALSGVVNI
                     VTQSGSDKFEGYATGYIGSYFTNSSDIFKNLDDPLNLAQKNLELTLSGPISPVKNLYF
                     FITGRYFTNEGYLYGERVFNVTDDIPYFPNPADQTFWIPQNTGDGKYISMNPYTKYST
                     NAKITYALPEINITYSLFYDDNENRYYDHGFSLTPDAIMTHYRTSLIHNLQFNYFPSS
                     NTFHSLKFSGNFYDYEGYLYPDAPIDTNVFSPTYNPYGIDPRYVNPDQGIPTSPYTFR
                     QGGNQVGRYNRHTNTLIAQYSYSSQISKEHKIGAGIEGKYYEIYSMNKDIINLTDGQV
                     DSMGNVIFTPGYPNKGTITDKGSYIEYTRNPFEFAAYVQDKMEYDILIINAGVRFDYF
                     NSNASLPADLRNPRHNPLYPGAVEDPNNPGNYMLEMVKADPKFQVSPRLGASFPITDQ
                     GIIRFSYGHFFKIPSFENLYNNPDFFVQPSSNVSSTTGNPNLNAEKNVIYEIGLQQVL
                     FENFALNFSLYYRDIRNWLGMEIINTYEGFKYARYINRDYANVKGFILTLDKRFANYF
                     GVKVDYTYQIAQGNASDPRAVFNNNQTNPPIEETKSVVPLDWDQRHTLNVNLNVGVPG
                     DWTVGMIFQYGSGQPYTEDPKISQGVRFENGGVKPTFYNVDLRADKIFDFNGFRINTY
                     LLVYNLFDIRNEFGVYTTTGRANVDLNANYYTQSDIIGLNTIPEYVNNPSMYSSPREI
                     RLGLGFGF"
BASE COUNT         1012 a          619 c          552 g         1088 t
ORIGIN      
        1 agcgggccac ttatcaggcc aggtatgtgg attagtatta attgcaggac tatttcgttc
       61 atttggatat tcatttacct gattgggatt tgaataaccc ggaactggtt ccaatcccca
      121 gatttctcct gtgctgggat ctttgtccat ccattcacga tatgaagtct gaagcggatg
      181 tacaatctga ttgttaccgg gggcggtaat ttccgacgct attaaaactg caacaccatc
      241 aagatacgaa tagccggtac ccttaggcca ctcaccactt ggctgatcag gccaatggcc
      301 aacctctcca ttattataaa aaagtgttct gatctgattg ccgtccataa taccctcttt
      361 acggaaatca atatttcctt taggctcatc tctatatttt tgagtttgag tgctcacctg
      421 tgagaaacct atatcagcaa tcaaaaaagt aataagaata aaaagtgttt tgtttaacat
      481 tattttctcc ggttatattc tctaaaaacc aaatcctaat cccagtctaa tctcacgtgg
      541 agacgagtac atactaggat tattgacgta ttctggtatc gtgttaagtc caattatatc
      601 gctttgggtg taataatttg cgttcaaatc aacatttgct ctgcctgtag ttgtataaac
      661 tccaaactca tttctgatat caaacagatt ataaactaac aggtatgtgt taattctgaa
      721 tccgttgaaa tcaaagattt tatctgctct aaggtcaaca ttataaaagg ttggttttac
      781 tcctccgttt tcaaatctta ctccctgtga tatttttgga tcttctgtgt aaggctgtcc
      841 tgatccatat tgaaatatca ttccaactgt ccagtcgccg ggcacgccaa cattaagatt
      901 aacatttaaa gtgtgtcttt gatcccaatc taatggcacc acactttttg tttcttcaat
      961 aggaggatta gtctgattat tattaaatac agcacgtggg tctgatgcat taccttgtgc
     1021 aatctgataa gtataatcga ccttcactcc aaaataattt gcaaatcgtt tatcaagcgt
     1081 taagataaat ccttttacat tagcataatc tctgtttata tatcttgcat atttaaaacc
     1141 ttcataggta ttaattattt ccatacctag ccagtttctt atatcgcgat agtaaagaga
     1201 aaagtttaat gcaaaatttt caaatagtac ctgctgcaaa cctatttcat atattacgtt
     1261 cttttcagca ttcaaattag gattgcctgt agttgatgaa acattactgc tgggctgtac
     1321 aaaaaagtcc ggattattat ataaattttc aaatgatgga attttaaaga agtgcccata
     1381 ggaaaatctg ataattccct gatctgtaat agggaaagaa gcacccaacc gcggactaac
     1441 ctgaaacttt ggatccgctt ttaccatttc taacatataa tttccaggat tattagggtc
     1501 ttctacagca ccgggatata gtggattgtg ccttggattt cttaaatctg ctggtaaaga
     1561 agcatttgaa ttaaaataat caaatcttac tccggcatta ataatcaata tatcatactc
     1621 cattttatcc tgcacatatg cggcaaactc aaatggattt cttgtatact caatataaga
     1681 acccttatcg gttatagttc ctttatttgg atagcccggc gtaaatatca cattacccat
     1741 gctgtctacc tgaccatcag ttaaatttat tatgtcttta ttcatactat aaatttcata
     1801 atactttcct tcaattcctg caccaatttt gtgttcctta gaaatttgtg acgagtagga
     1861 atattgagca atcagagtat ttgtatgacg gttatatctg cctacctgat taccaccctg
     1921 tctaaaagta tatggagaag tcggaattcc ttggtcgggg tttacatatc ttggatcaat
     1981 tccgtaagga ttgtaagttg ggctgaatac atttgtatca ataggtgcgt ctggatataa
     2041 ataaccttca taatcataaa agttacccga aaactttaat gaatgaaaag tgtttgaact
     2101 tggaaaataa ttaaactgta aattatgtat taagcttgtt ctgtaatgcg tcattatagc
     2161 atccggggtt aatgaaaaac catgatcata gtatctgttt tcgttatcat cataaaacaa
     2221 actgtatgtt atattaattt ccggaagagc ataagttatt tttgcattcg ttgaatattt
     2281 agtataagga ttcatagaaa tatatttgcc atcacccgtg ttctgtggaa tccagaaagt
     2341 ctgatcagca ggattaggaa aataaggtat atcgtcggta acattaaaaa ctctttcgcc
     2401 gtataaatat ccttcgttag taaaatatct tcctgttata aaaaagtata aatttttaac
     2461 gggggaaata ggtccgctta aagtaagttc taaattcttt tgtgcaagat taagcggatc
     2521 atctaaattc ttaaaaatat ctgaactatt agtgaaataa cttccaatat aaccggtagc
     2581 gtaaccttca aacttatcag atccgctttg tgtaactata ttaacgacac cggaaagcgc
     2641 ctgtccatat tcagcattaa aagtaccgct tataacttcc atctgtcgaa cggatgaatt
     2701 ttcgacctgt acgccaaatc cgccgttaaa tgcatctgta actgcaacac catcaatcaa
     2761 ataagcaaca tcattagatc tgccgcctct gaagtgacca tcaataaccc ctgcctgcag
     2821 atttacaatt tgtccgatgc tctcaacagg catcatttta atttcgttag aagatattgt
     2881 aactgatgtt gaggtaagat ctttttgaac gagtggtctt tgtgaggtaa caacaacttc
     2941 ctgattcaat tgtataacag agctgcttag tttcatatca agatttgtag taagatctat
     3001 atttacgcga actttttcta caatgatttt ttgatatccg actgcgctga atataacacg
     3061 atattcaccg ggtggaatat tgctgatgga ataatatcct tccagatcag cagaggcacc
     3121 cagatatgtt ccttcaataa taatgtttgc accaacaatg ggttcaccag tttcctggtc
     3181 tgtaacagta ccggaaattt tgcctgttgt tcctgcaaac aatggaacgg ttaataatag
     3241 cactactaac ggaataaaaa atcgtagtat t
//
LOCUS       DASWBY010000031         4330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_490379, whole genome shotgun sequence.
ACCESSION   DASWBY010000031 DASWBY010000000
VERSION     DASWBY010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490379"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VF870_00575"
     CDS             complement(<1..658)
                     /locus_tag="VF870_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250698.1"
                     /translation="MKNLILIIIFSVAFLYPQNITVDNNKDFEKALSLYKSKKYDDAF
                     YLFKNVASRTENNTKNTASAFFVSKILVEKKKYTDAEKSITNFLKNYPQSKYADEVKN
                     LLIKTYVDKAEFEKAFETSLNFIESSGSIVFKKETKYIAEKIALGNLNSSDVEKYIDK
                     YSSLKPFLILLTGKLYLAEGDNSDAVKKFDEITSQYLTSEEYVEALNLKKTQIDSQTN
                     S"
     gene            complement(668..1537)
                     /locus_tag="VF870_00580"
     CDS             complement(668..1537)
                     /locus_tag="VF870_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HEX9250699.1"
                     /translation="MEEDRIKFKIKLASVFISEGKNLHAIQVYKNLIEDTDREEFYFQ
                     LAELYEDMGFVDAGKNILSELLIKNNDDKDAKFYFGQYLLRNSRWIEAINTLNSISAL
                     TPEALFLIAYSYMMLNEFELAKEYFSNFIASDEKNDLKQEANLYLAKIEYELHNYNSA
                     LNYATNAQYFYSDFWELNLILAKVYYSLDMFTHAVNPIQKALKLNHKDPSVQEFAGKI
                     YFQLQDYKKAEKFFSEFIELSSEVSAEIYTLLAKSFVKQRKIDEANLFFELALQIDPA
                     YQPAISGKGDLNK"
     gene            complement(1538..3079)
                     /gene="guaA"
                     /locus_tag="VF870_00585"
     CDS             complement(1538..3079)
                     /gene="guaA"
                     /locus_tag="VF870_00585"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX9250700.1"
                     /translation="MKQKEKILIIDFGSQFTQLITRRVREAQVYSEIHSHKITFEEIK
                     KYNPAGIILSGGPMSVYDTDAPDLDERILKMNVPVLGICYGLQLISKKLGGKVEPAKD
                     REYGKAILNVVGTSPLLNNVQKDSVVWMSHGDFLTELPKGFSIIANSDHSPIGAAANE
                     SEKIYGVQFHPEVAHTVEGKKIIHNFLFDICKCAGDWTSQNFIENSIAEIKEKAGNAK
                     VICALSGGVDSTVAAVLVKKAIGDNLICIHIDTGLMRKNESYEIGKIFNEKLDLKHIH
                     INASEKFISKLKGISDPEQKRRIIGNTFIEIFEEEAKKIHDAQFLVQGTLYPDVIESV
                     SVKGSSVTIKTHHNVGGLPEKMNLKLIEPFRSLFKDEVRNVGRELNIPEEFIERHPFP
                     GPGLAVRILGAVDQEKLDILKEADDIYIKAIKSAGIYNKIWQAFAVLLPVQSVGVMGD
                     ARTYEFVLALRAVTSVDGMTADWYNFEHNFLSDVSNKIINKVRGINRVVYDISSKPPA
                     TIEWE"
     gene            complement(3158..3538)
                     /gene="gcvH"
                     /locus_tag="VF870_00590"
     CDS             complement(3158..3538)
                     /gene="gcvH"
                     /locus_tag="VF870_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX9250701.1"
                     /translation="MSVPNNLKYTKEHEWVLVEGSTGTIGVTDYAQGELGDVVYVDID
                     PSLAEVKKGESIGTIEAVKTVSDIFAPYSGKVIEINQAVRDAPEVVNIDPYGNGWMIK
                     VEISDASELSDLLDASAYQALIGQ"
     gene            complement(3570..>4330)
                     /locus_tag="VF870_00595"
     CDS             complement(3570..>4330)
                     /locus_tag="VF870_00595"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559676.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9250702.1"
                     /translation="GDVYIEKYLENPRHIEFQVMGDSFGNVLHYGERDCSVQRRHQKL
                     IEESPSPALDEQLRTKMGEAAVLGAKSVNYLGAGTIEFLLDKHKNFYFMEMNTRIQVE
                     HPVTESVYSVDLVRQQILVAAGKKVDVAPQKPFGHSIEFRINAEDPDHDFRPSPGKIT
                     SLNFPGGFGVRVDSHIYQSYTIPPYYDSLVAKLIVWGRTREKALARGKRALEEFVIEG
                     IKTTIPFHLKVLQDPRFISGNFDTGFLEKFNADN"
BASE COUNT         1350 a          824 c          625 g         1531 t
ORIGIN      
        1 cggaatttgt ttgcgaatca atctgtgtct tttttagatt aagtgcttca acatattctt
       61 cagaagtaag atactgtgat gtaatctcat cgaatttttt gacagcatca ctgttatcac
      121 cctcagctaa atataactta cctgtcaata gaattaagaa aggcttaagc gatgaatatt
      181 tatcgatata tttttcaaca tctgaactat taaggttacc caacgcaatt ttttctgcga
      241 tgtacttagt ctccttctta aagacgattg aaccagatga ctcaataaaa ttaagagaag
      301 tttcaaaagc cttttcgaat tctgctttat caacataagt ttttattaaa agatttttaa
      361 cctcatccgc atatttgctt tgaggataat tctttagaaa attagtaatg gacttttcgg
      421 catcagtata ctttttcttt tcaactaaaa ttttagatac aaaaaaagct gaagcagtat
      481 tttttgtatt attttccgtc ctgcttgcaa cgtttttgaa aagatagaac gcatcatcgt
      541 actttttaga cttatataag ctgagtgctt tttcaaagtc cttgttatta tctacagtaa
      601 tgttttgcgg atataaaaaa gcaacagaga agattattat taaaatcaaa tttttcatat
      661 caatagatta tttattcaga tcacctttac cggaaattgc gggttgataa gccggatcaa
      721 tctgtaacgc caattcaaaa aatagattag cttcatcaat ttttctttgc ttaacaaagg
      781 atttggctaa cagagtatat atttctgcag aaacttcaga acttaattca ataaattctg
      841 aaaaaaactt ttctgctttt ttataatcct gcagctgaaa ataaatcttt ccggcaaact
      901 cctgcaccga tggatcttta tgattaagtt taagtgcttt ttgtattgga ttaactgcgt
      961 gtgtaaacat atctaaactg taataaactt ttgcaagtat taaattcagt tcccaaaaat
     1021 ccgaataaaa atactgagca tttgttgcat aatttaaagc cgaattataa ttgtgcagtt
     1081 cgtattctat cttagcaagg tagagatttg cttcctgttt cagatcgttt ttttcatctg
     1141 aagcaataaa atttgaaaaa tattcttttg ctagttcaaa ttcatttagc atcatataag
     1201 aatatgctat taaaaacaat gcctcaggtg ttaatgctga tatactgttt aatgtattta
     1261 ttgcttcaat ccatcttgaa tttctaagaa gatattgccc aaagtaaaat ttagcatctt
     1321 tgtcatcgtt atttttaata agcagttcag atagaatatt ttttccggca tcaacaaatc
     1381 ccatatcttc atatagttca gcaagttgaa aataaaactc ttctctatca gtatcttcaa
     1441 tcagattttt ataaacctga attgcatgaa gattcttccc ttccgagata aaaactgaag
     1501 ctagttttat tttaaatttt atcctgtctt cttccactta ttcccattca attgttgcag
     1561 gcggcttaga gctaatatca taaacaactc tgttgatacc acgcacttta ttaatgattt
     1621 tatttgaaac atccgataaa aaattatgtt caaaattata ccagtctgca gtcatcccat
     1681 caacagaagt tacagcgcgc agggctagca caaattcgta agttcttgca tcacccatta
     1741 ctcctactga ttgaaccgga agcagcactg caaatgcctg ccaaatttta ttgtaaattc
     1801 cggcactttt aatagcttta atataaatat catctgcttc ttttaaaata tcaagttttt
     1861 cctgatcaac tgcaccaaga attcttactg ctaaaccggg accaggaaat ggatgtcttt
     1921 caataaattc ttcaggaatg tttagttctc tgccgacatt ccttacttca tctttaaaca
     1981 aggaacgaaa gggttcgatt aatttgaggt tcatcttttc cggtaaaccg ccaacattat
     2041 gatgtgtctt gattgttacc gatgatcctt ttacagaaac ggattctatt acatcgggat
     2101 agagagttcc ctgaacaaga aattgtgcat catgaatctt tttagcttct tcttcaaaaa
     2161 tttctataaa ggtgttgcca attattcttc ttttttgttc cggatccgaa atacctttta
     2221 atttactaat aaacttttct gaagcgttga tatgaatatg tttaagatcc aatttttcat
     2281 taaatatttt tcctatttca taactttcat ttttgcgcat caatcctgta tcaatatgaa
     2341 tacaaatcag attatcacca atggcctttt taacaagcac tgctgcaaca gttgaatcaa
     2401 caccaccact taacgcacaa ataacttttg cattgcctgc tttttccttt atttcagcaa
     2461 tactgttttc gataaaattt tgagatgtcc aatcaccggc gcacttacaa atatcaaata
     2521 aaaaattatg tattatcttt tttccttcaa ctgtatgggc aacttcagga tgaaattgga
     2581 caccataaat tttttcagat tcatttgctg ctgctccaat aggtgagtga tcagaatttg
     2641 caattatgct gaatcctttt ggaagttctg taagaaaatc accgtgactc atccaaacta
     2701 ctgagtcctt ttgaacatta ttaagtaagg gagaagtccc gacaacattt aaaatagctt
     2761 ttccatattc tctatcttta gctggctcaa ctttgccgcc aagtttttta ctaattagct
     2821 gaagtccgta acaaatacca agcacaggaa cattcatttt aagtattcgt tcatcaagat
     2881 caggtgcatc agtatcataa acactcattg ggccaccgga tagaatgata ccagctggat
     2941 tgtacttttt tatttcttca aaagtgattt tatgagaatg aatctccgag taaacctggg
     3001 cttctcttac ccttcttgtt ataagctggg taaactgtga gccaaaatct attattagta
     3061 ttttctcttt ttgtttcatc tagttcttct ggtttaaaaa taaaaaaaca aaactcagtc
     3121 acccgaattt tgtttttaat tttttcaaag aaaattatta ttgaccgatt aacgcctgat
     3181 aagcagatgc atcaagcaaa tctgataatt ctgaagcatc gcttatttca actttaatca
     3241 tccagccatt tccataagga tcgatattta caacttcagg cgcgtctctt acagcttgat
     3301 taatttctat cactttgccg ctataaggcg caaaaatatc acttacggtt tttacagctt
     3361 cgattgttcc gattgattct ccctttttaa cttcagctaa agaaggatcg atatcaacgt
     3421 aaacaacatc gcccaactca ccttgagcat aatcggttac accaatcgtg cctgtgctgc
     3481 cttcaacaag aacccactca tgttcctttg tatattttag attatttgga acgctcattt
     3541 tatcctctta aaattatctg aataaaccat taattatctg cattaaattt ttcaagaaat
     3601 cctgtatcaa agtttccgct aataaaacgg ggatcctgaa ggactttcaa gtgaaaaggt
     3661 atcgttgttt taattccttc aattacaaat tcttcaagtg cacgtttacc tcgtgctaaa
     3721 gctttttctc ttgttcttcc ccagacaatt aattttgcaa ctaaagaatc atagtatggc
     3781 ggaatagtgt aagattgata aatgtgtgaa tcaactctaa caccaaaacc tcctggaaaa
     3841 tttaaagagg taatttttcc tggtgaaggt ctaaaatcgt gatccgggtc ttcagcatta
     3901 attctaaact caatactatg tccaaaaggt ttttgcggag cgacatcaac cttttttcct
     3961 gctgctacta aaatctgctg tcttacaaga tcaacgctgt aaactgattc tgttacagga
     4021 tgctcaacct gaattcttgt attcatttcc ataaagtaaa aattcttatg tttatctaat
     4081 aagaattcaa ttgtgcccgc accaagataa tttactgact ttgcaccaag tacagctgct
     4141 tcacccattt ttgttctaag ttgttcatca agagctggtg atggtgattc ctcaattaat
     4201 ttttgatgcc gcctttgcac agaacagtca cgttctccat aatgcaatac atttccaaaa
     4261 ctatccccca ttacctgaaa ttctatatgt cggggattct ctaagtattt ttcaatataa
     4321 acatcgccat
//
LOCUS       DASWBY010000032         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_715851, whole genome shotgun sequence.
ACCESSION   DASWBY010000032 DASWBY010000000
VERSION     DASWBY010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715851"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..822
                     /locus_tag="VF870_00600"
     CDS             58..822
                     /locus_tag="VF870_00600"
                     /inference="COORDINATES: protein motif:HMM:NF018659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HEX9250703.1"
                     /translation="MFRNILQKFFILQLFIISIYIGCSRENPESITNPTSIQKIYPIN
                     VYALTIPEPSGVAYNSKNNSLMVVSDGNPDIFEIDFTGITLNTIIASGSDMEGITLSK
                     NCDTIYVVEEKKKLVTTFDLNGKKLNSFSINVATSDNNALEGITINTTSNQLFIINEK
                     NPRMIIGYSNLTEFWRKSIDYALDISDIYFEDSTNCFWLISDESQKIMKLSSTGDLLK
                     EWEIPFTKGEGITIVGDKIYVVNDLDGKLYVFQKPI"
     gene            864..>1704
                     /locus_tag="VF870_00605"
     CDS             864..>1704
                     /locus_tag="VF870_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX9250704.1"
                     /translation="MKKNYLLLFVFLTAFFMLQSGFAQTLVMNEIYSRGVSGNLDWIE
                     IYNPALTPIDISDYKIYDSGGQSGSKPKKLFPSGTIIPAQGFYVIITDTADFDGDLSG
                     FGLSSSGEEVWLENASGTVIDNVTFPAMAVTESYGRLPDGGTWQLLSTITRGASNFVV
                     EVNYTLMNELFSRGTSAEPDWIEIYNSSNTEKDISGYKIYDSGGQAGTKPKKEFPSGS
                     IIPANGYLVIVVDDADPSGFGLSSNGEWVWFENASGTVIDSINLVAVTDTAASYGRLP
                     DGSD"
BASE COUNT          582 a          256 c          327 g          539 t
ORIGIN      
        1 tataaattat aagagaacca gactaagctc tggttctttt tatttttagg aatataaatg
       61 tttagaaata ttctacaaaa atttttcata ctgcaattat ttattatttc catctatatc
      121 ggatgttcaa gagaaaatcc ggaaagtata actaatccta cttctattca aaaaatatat
      181 ccgataaatg tatatgctct aactattccg gaaccatcgg gcgttgctta caattctaaa
      241 aataattctt taatggtagt ctctgatgga aatccagata tatttgaaat tgacttcact
      301 ggaattacat taaatacaat tattgcctct ggatcagata tggaagggat aacactttct
      361 aaaaattgtg atacaattta tgtggtggaa gaaaagaaaa agctagtaac aacttttgac
      421 ttaaatggaa aaaagttaaa ctcatttagc attaatgttg ccacaagtga taataatgca
      481 ttagaaggga taacaattaa tacaacatct aatcaattgt ttataattaa tgaaaaaaat
      541 ccgcgaatga ttattggata cagtaattta acagaattct ggcgcaaatc gattgattac
      601 gcattggata tttctgatat ttactttgaa gattcaacaa attgtttttg gctgattagc
      661 gatgaatctc aaaaaattat gaagctatcc tccacagggg acttacttaa agaatgggaa
      721 atcccattta caaaaggcga gggaattaca atcgtaggtg ataagatata tgttgtcaat
      781 gatttagatg gcaaattgta tgtgtttcaa aaacctattt aactagtagt agatcattat
      841 gtttatttta taaaggagtt cttatgaaaa aaaattattt acttttattt gttttcctaa
      901 cagcattttt tatgctgcaa agcgggttcg cccagacact agtgatgaat gaaatatact
      961 ctagaggagt ttccggcaac cttgattgga ttgaaattta taatccagca ttaaccccaa
     1021 tagatataag cgattataaa atttatgata gcggtggaca aagcggctca aaaccaaaaa
     1081 agttatttcc atctggaaca ataattcctg ctcaaggttt ttatgtgatc attactgata
     1141 cagcagattt tgatggagac ctcagtggat tcggcttatc cagcagtggt gaagaggttt
     1201 ggctggaaaa tgcttccggg acggttatcg acaatgtcac ttttcccgca atggcagtta
     1261 ccgaatctta cggacgatta ccggatggtg gtacctggca gcttctaagt acaattacaa
     1321 ggggggcctc aaattttgtt gttgaagtta actatactct aatgaatgaa ttattttcca
     1381 gaggtacatc ggcggaacca gattggatag agatttataa ttcatccaat actgaaaaag
     1441 atatttcagg atacaagatt tatgatagcg gtggacaggc tggaacaaaa cccaaaaagg
     1501 aatttccttc aggaagtatt atccctgcta atggttacct tgttatagtt gtagatgatg
     1561 cagatccaag cgggtttggg ttgtcaagca acggagaatg ggtatggttt gaaaacgctt
     1621 caggaacagt tatagacagt attaatcttg ttgcagtaac tgatacggct gcctcttatg
     1681 gaagattacc agacggaagt gata
//
LOCUS       DASWBY010000033        14984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_339732, whole genome shotgun sequence.
ACCESSION   DASWBY010000033 DASWBY010000000
VERSION     DASWBY010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 14984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14984
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339732"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..568)
                     /gene="rpsI"
                     /locus_tag="VF870_00610"
     CDS             complement(182..568)
                     /gene="rpsI"
                     /locus_tag="VF870_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX9250705.1"
                     /translation="MAEKIFIGRRKTAVARVILRSGNGKITVNDKEFEHFFPQLLSRE
                     DILLPFRATDTLGKFDVHANVNGGGTTGQAQSVRLGIARALLEMNPEYRVQLKAEGLL
                     TRDPRMVERKKYGQPKARKRFQFSKR"
     gene            complement(568..1017)
                     /gene="rplM"
                     /locus_tag="VF870_00615"
     CDS             complement(568..1017)
                     /gene="rplM"
                     /locus_tag="VF870_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX9250706.1"
                     /translation="MKQEKLTKSVKTEDANHKWFLVDAEGQVLGRLASKIAMIIRGKN
                     KPIFTTNTDTGDFVVVINAEKVKLTGKREELKEYKRHSGYPGGQTTTSYREMLAKKPE
                     FVLESAVRGMLPKTKLGNKLIKKLKVYAGTNHPHAAQQPEAISLMEK"
     gene            complement(1163..1891)
                     /locus_tag="VF870_00620"
     CDS             complement(1163..1891)
                     /locus_tag="VF870_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250707.1"
                     /translation="MNKQEFELENINDNKNWIRNFSISIIIHLVVVAVFIFLYGLKDD
                     KARINSMFFTIDTKEFVDPNTNLNDDQKNNSTSQAQVLPEKQTSETNQELKSISYSDI
                     TADTTNLDQIYKEPTLNVSIKYPKGWTFIDQKKKKKLDGVTFWASDGQYNPPPYIHLE
                     VVSSDMFIEKRFSYKMELEKYQAFYNDPEEMQGYITQIIYIRTGNEEDFKLKLMMKGK
                     TEFLSFQPKFWAILKSFNFGSNLF"
     gene            1972..3009
                     /locus_tag="VF870_00625"
     CDS             1972..3009
                     /locus_tag="VF870_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9250708.1"
                     /translation="MKYLITGGAGFLGINLVRYLHSKGHEITSLDIVEFDYPDMNDKI
                     KIITGDIRDKAKVKEAVNNQDIIVHTAAALPLYKPEDIFSTDIDGTRNLLEAADENNI
                     KRFIHISSTAVYGIPDHHPLLENDKLDGVGPYGKAKILAEEECIKYRNKGMCVPILRP
                     KSFIGPERLGVFDLLFDWAQDGKGFPMIGNGKNRYQLLDVEDLCLAIYLTATLDEKIV
                     NDTFNIGAKEFTTMREDYQAVLDYAGFGKKIVGLPEKPIIYTLKFLEALKLSPLYKWV
                     YETASKDSFVSIEKAEKILGYKPKFSNKDALLRNYKWYVEHRNEFINKDGVSHRVPWK
                     QGILRFAKIFF"
     gene            3089..4132
                     /locus_tag="VF870_00630"
     CDS             3089..4132
                     /locus_tag="VF870_00630"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX9250709.1"
                     /translation="MAEKKDYLKEIVKHIDIKQHNVVPLVDAMEDMAFTARDLNRAAN
                     ILDTMINDKDCAVILTLAGSLFSAGLKKVVYDMIMNNMVDAIVSTGAIMVDQDFFEAL
                     GFKHYKGTKWVDDNEMRELHIDRIYDTFIDEDELRICDDTTALIFDSMKPGAYSSREF
                     LWEFGKYLDENGGPKVDDSVVYAAYKKNVPIFIPAFSDCSAGFGFVMHQHKNPDNHVS
                     CDSGKDFLELTKIKLHSKDTGILMIGGGVPKNFTQDIVVAADILQEDAEMHKYAIQIT
                     VADERDGALSGSTLKEASSWGKVSTSSEQMVYAEATVAMPLIVGYAYHKGGWKNRKAR
                     EFQKIYSKENAIA"
     gene            4410..5753
                     /gene="trkA"
                     /locus_tag="VF870_00635"
     CDS             4410..5753
                     /gene="trkA"
                     /locus_tag="VF870_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEX9250710.1"
                     /translation="MRIIIAGMGDVGYQLAKQLSNESHDIIAIDTNLERLSYSDQMAD
                     ILTIKGSSTSIKILNEAGVSKADLLVAVTSSEEVNIATSIIAKKLGAKKTIARISNAE
                     YLDPTTKINFVEIGVDFMIYPEELAAIETVSLINRTAATDVLEFEDGKLAVIGLRLDK
                     NAPIANKKLAEIANEFQNTDFRVVAIQRNFKTIIPKGNDKFLPNDQVFVITKPEGHDQ
                     ILKLAGKENIKFDNIMVLGGGKIGRQIASLLSDKMQVKLIESDPDKTFELADQLPDTL
                     VIKGDGRDIDLLAQEGIIDVDAFIAVTDDAETNIISCLLAKHLGVKKVIALVDKVDYI
                     PLTQTIGLDSLINKKLIAANNISRFIHKTDVIVIATLEGIDADVLEFNVQPNSAITKN
                     QISEIDFPKGSIVGGFIRGDEVSIATGDSRINAGDKVVVFSLPDSVSKLEKIFKR"
     gene            5762..7210
                     /locus_tag="VF870_00640"
     CDS             5762..7210
                     /locus_tag="VF870_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX9250711.1"
                     /translation="MNYKIIINIIGFLIILTGIFMMLGLPFSIYYKSNDIPVILFSGI
                     TTSSIGFTAWFTTRKTDRHEVGKREGYIIVTLGWILMSAFGAIPFVIHGSISSYTDAF
                     FETVSGFTTTGASILPKVEVLPYGLMFWRSMTHWIGGMGIIVLSLAILPLLGIGGMQL
                     YQAEVAGPTKDKLHPRVRETAKRLWAIYFFLTSAEVILLLFGNMNLFDSLCHSFGTLA
                     TGGFSTRTQSIAYYHSAYIEYVILFFMFLGGTNFTLHYLALHGKINSYFKDDEFKFYL
                     SLIIITVTALTIYLILADGTSLESTFRKVAFSVLSILTSTGFATADYELWAPFSSMLF
                     LIILLLGACAGSTSGGVKMIRYQLLIKNSITEIRKLLHPNAIVPVRHNGRSVPGDIIA
                     KVSAFVLTYLAIFVIGSLMLSITGLDLKTSMGSVAACMANIGPGLGLTGPAGNYSQVS
                     DFGKWLLSALMLLGRLELFTVLIIFTPYFWKK"
     gene            7327..9462
                     /gene="ppk1"
                     /locus_tag="VF870_00645"
     CDS             7327..9462
                     /gene="ppk1"
                     /locus_tag="VF870_00645"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX9250712.1"
                     /translation="MNTKELLKKYSKPENFINRDLSWVEFNKRVLEEALNPELPLLEK
                     IKFISIFCSNLDEFYMIRIAGIKEQIAANVEEPSIDGLTPIEQLRKIEKTLKPLLKNL
                     DNYWTNYIIPSLKENSVKLLTMDDFSEGEKTKLREYFKREIYPVLTPLAFDPGRPFPY
                     ISNLSLSLAILIRKPNGENHFARVKVPSILPRLLQIDNIIEPNKSPGVNGNFTATYIW
                     LGDLIKENLPLLFPGMEIVEAHRFRITRDTDIELQEDEADDLLSVIEENIKQRRFGSV
                     VRLEVGHPVPDFMLDTLIDNLQITKDDVHVSDGPLGLSDVMSLFKLPLHQLKEKPFYP
                     VIPKVFEDEEDYFSIIRQKDILLHHPYHSFSPIIDFIKQAALDPDVLAIKQTLYRVGP
                     DSPIVRCLIEAAEMGKQVAVLVELKARFDEQNNIYWARALEKVGVHVVYGLVGLKTHA
                     KMTLVVRKEYDGVKRYVHLATGNYNATTAKLYTDLGFFTSDDDICSDVSDIFNFLTGY
                     SKQKEYKKLFVSPVNMRERFIDLINREIDNALAGKEAKIIMKLNSLVDPAIISALYEA
                     SNSGVEIKLIVRGVCSLIPGVKNLSENIEVRSIVGRYLEHSRLFYFYNDGTEEIYLSS
                     ADMMQRNLDRRVEVAFPIENQKLKDELIKTLIKVALKDNVKARILQPDGSYIMAEEKE
                     STKKISSQEWLMNHALKAMGVRSKKPFGK"
     gene            9503..10648
                     /gene="apbC"
                     /locus_tag="VF870_00650"
     CDS             9503..10648
                     /gene="apbC"
                     /locus_tag="VF870_00650"
                     /inference="COORDINATES: protein motif:HMM:NF008669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX9250713.1"
                     /translation="MSFSLFKKKQESKMFEITKSTVINSLKNISNDRINTGLFTLNSI
                     RNFEINNDDLSLEISVNADNTEELKELIKNQLKKDFSGLKNINLTMKTKSSSTVSTHK
                     DARKDAILPGVKNTIAIASGKGGVGKSTVAVNLAVALAKDGASVGLIDADIYGPSIPI
                     MLGSKDKPKVYQAENSVKMIPLENHGIKFMSIGLLVDDKDPIIWRGPMASGAIKQFMT
                     DVDWGELDYLIFDLPPGTGDIQLTLVQTIPLTGAVIVTTPQEVSLIDARKALKMFERV
                     NVPVLGVVENMSYFIAPDTGNKYDIFGSGGGENICKDLNTTFLGGLPINPGIRQGGDS
                     GVPMVFGIPDSNETKILMDISRKLTEQVNIRNSNASDKIEILLGDED"
     gene            10722..10946
                     /locus_tag="VF870_00655"
     CDS             10722..10946
                     /locus_tag="VF870_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX9250714.1"
                     /translation="MKNEVQKALNNIRPYLQADNGDVELIDVSDDGIVKVKLLGACEI
                     CPLSIMTLRAGIERAIMREVPAVKRIEAVS"
     gene            10954..12003
                     /locus_tag="VF870_00660"
     CDS             10954..12003
                     /locus_tag="VF870_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9250715.1"
                     /translation="MDLLNKLNIRFFLLLLAIVVLFYFSYLLINVLVLIAISVLLGFI
                     FAPFVRTVEGQGFSRTISTLIVFIIFAFLLYLGLSFVIPKFYSQMDQVIISLKDFSLN
                     EELNDLEKKILSVFPLFNKGELSQKVQSIISTSFNDSINHITEYVSSVVSVAAFLVIV
                     PFITFYLLKDSAVIQKGLIHIVPNKYFEMSYWILKRISLQLGRFVRGWIFDATFVGIA
                     IGFGFYFIGLPNALPLGVIAGIGHLVPYLGPVIGGIPAIVLSIIQTGDLSQVPLIIMI
                     VALTYTLDNGFVQPYVFSKSVDMHPIIIILLIIIGSELFGIIGMLLAVPTATVIKTAA
                     KEIYFAYKNYSIAKI"
     gene            complement(12015..13013)
                     /locus_tag="VF870_00665"
     CDS             complement(12015..13013)
                     /locus_tag="VF870_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9250716.1"
                     /translation="MNELSSVNNDVQLVEQLNKKVTEIKSEIAKVIVGQDEIINQLLI
                     SLLSRGHCLLVGVPGLAKTLLIKTLAEVMDLKFSRIQFTPDLMPSDITGTEILEENLA
                     TKKRNFRFISGPIFANIILADEINRTPPKTQAALLEAMQEHKVTAAGQTYQLPEPFFV
                     LATQNPIEQEGTYPLPEAQLDRFMFNLWLDYPSVEEEIKVVQTTTSEYKATLKKVVSS
                     DELIMFQDLVRKVPVAPNVIEFAVKVSNMTRPMNGNAPKYVKDWVTWGAGPRASQYLV
                     MAAKTRSIIQGRFTPNIDDIKASMLPVLRHRIITNFSAEAEGISSIDVINRVETEA"
     gene            complement(13030..14301)
                     /locus_tag="VF870_00670"
     CDS             complement(13030..14301)
                     /locus_tag="VF870_00670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9250717.1"
                     /translation="MKIKIAALILLFAGVISPQKVLDKIVAVVDNEIILQSELDFQVG
                     IFASQRQIDPNTPGLKEQLLNTMIEEKLIYAQADLDSITVTEEEINQRIDYQINSFTQ
                     QYGSVANIEKIYGMSIDRIKRELRDEVRKSLMSQRLQEKNFGKVQATRREVEDFFSTY
                     KDSIGNIPEKVTIFHIFQNPKASEKLKKKFYDKALALLDSLKAGKDFAELAKKYSDDP
                     GSAAQGGDLGFVKKGVFYPEFEEAAFKLNVGELSGVVESPVGFHIIQMIEKRGESIHT
                     RHILIKIKADENADLQTIDFLSAIRDSILQGKGTFQEFAKKYSEDKETAPFGGELGNF
                     YISQLDKALLDAVGKLKEGDIGFPRRLEYAPGTYGYHIVWLKSRVPQHKANLTDDYDE
                     IKKLADEHKKQLEYTKWIEEIKSKIYWEVRI"
     gene            complement(14298..>14984)
                     /locus_tag="VF870_00675"
     CDS             complement(14298..>14984)
                     /locus_tag="VF870_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9250718.1"
                     /translation="FASLADTSNLNSAQKEQLIKNWIYQEILFQAADKEGITKRKDYL
                     SIIKKSSNELAASMLIDDYLANEEIKYSDDDINNYYESSKNYFRLNSDSYLINKVTFN
                     SEDNAIKFRTLALESEWKKAVSVFVNDSSLVKNVLSDLVEESNIYPMQLSRIVKDFYP
                     EEISIVITEKPGYYSVVQLLGKYGKQSIPPFEIIKPTVEKRFLAEKKKLLVEEYLRDL
                     YSRNEIEIKK"
BASE COUNT         4830 a         2457 c         2600 g         5097 t
ORIGIN      
        1 gttagatcaa cttttttcat attgctcctg tttatcttct actttcttca actttacctt
       61 ccatcccttt tattatggga cacaggtggt aaatcggaaa gtatgattat taaataaaag
      121 ttcaagttta agatcaagtt caagaaaaat cttgaacttg aacccatact taaactcgtg
      181 attatctttt agaaaactga aatctttttc ttgcttttgg ctgtccgtat tttttacgtt
      241 caaccattct tggatctctt gttaataaac cttctgcttt taattgaact ctgtattcag
      301 gattcatttc aagcaatgct cttgcaattc ctaatcttac ggattgtgcc tgacctgttg
      361 ttccgccacc gttaacattt gcatgaacat caaatttgcc tagtgtgtca gtggctctaa
      421 atggcagcaa aatatcttca cgacttaata actgtggaaa gaaatgttca aactctttat
      481 catttacagt aatttttcca ttaccgcttc ttaatattac gcgggcaact gcagtttttc
      541 ttcttccaat aaatattttt tcagccatca tttctccatt aaactaattg cttcgggttg
      601 ctgtgctgca tgcggatgat tagtacctgc atacactttt aattttttaa tcaacttatt
      661 tcctaatttg gttttaggca acattcctct aactgcactt tctaaaacaa attcaggttt
      721 ctttgctaac atctcacgat atgaagttgt tgtttgaccg ccaggataac ctgagtgtct
      781 tttgtattcc ttcaactctt ctctttttcc agtcagttta accttctcag catttatcac
      841 aactacaaaa tcgccggtat cagtattagt agtaaagata ggtttatttt tacctctgat
      901 aatcatagca atttttgaag ctaatcttcc taaaacctgt ccttcagcat caactaaaaa
      961 ccatttgtga ttggcatctt cagtttttac tgattttgtt aatttttcct gtttcacgta
     1021 attttcctcc gaaaacctta aatcgtttca aataaaggat ggaaaaatat aggtaagatg
     1081 atgaaaagtc aagaatattg tagtttatta ataatcgcta acttaatgtg tgatatttaa
     1141 tctaaaaacg acattaagaa tactaaaaca aattgcttcc aaagttgaat gatttaagta
     1201 ttgcccagaa ctttggttga aatgacaaaa attcagtttt ccccttcatc atcagcttaa
     1261 gtttaaaatc ttcttcattt ccggttctta tgtaaattat ttgagtaata tatccctgca
     1321 tttcttcagg gtcgttataa aaagcctggt atttttccag ttccattttg tatgaaaatc
     1381 ttttttcaat aaacatatca ctgctaacta cttccagatg aatatacggc ggcggattgt
     1441 attgcccgtc gcttgcccaa aatgtaaccc catcaagttt tttctttttc ttttgatcta
     1501 tgaacgtcca gcctttggga tattttatcg aaacattcaa ggttggttct ttataaatct
     1561 gatctaagtt agtggtgtca gcagttatat cagaataact aattgatttt aattcctgat
     1621 ttgtttcaga agtttgtttc tcaggtaata cctgtgcttg acttgtagaa ttattctttt
     1681 gatcatcatt taaatttgta ttaggatcta caaactcctt tgtatcaatt gtaaaaaaca
     1741 tggaatttat tcttgcttta tcatctttca atccatataa aaagataaat actgcaacaa
     1801 caaccagatg aataattatt gaaatagaaa aatttcttat ccagttttta ttatcattta
     1861 tattttcaag ttcaaattct tgtttgttca tatacactat aaagctaagt ttgatgcctg
     1921 aatcaaaatt actaattttg gacaaacatt attaacaaat tatagcgcaa aatgaagtat
     1981 ctaattacag gtggagctgg atttcttgga ataaatctgg taagatattt acactccaaa
     2041 ggtcacgaaa taacttctct tgatattgtt gaattcgatt atcccgatat gaacgataaa
     2101 attaaaatca taaccggaga tataagagat aaagcaaaag ttaaagaagc agtaaacaat
     2161 caagatataa ttgtgcacac tgcagcagct cttccgcttt ataaaccgga agatattttt
     2221 tccaccgata ttgacgggac aagaaactta cttgaagctg ctgatgaaaa taatattaaa
     2281 agatttattc acatttcatc aactgctgtt tatggtattc cggatcatca tccattattg
     2341 gagaatgata aacttgatgg ggttggtcct tatggaaaag caaaaattct tgctgaggaa
     2401 gaatgtatta agtatagaaa caaaggaatg tgtgtcccaa ttcttcgtcc taaatctttt
     2461 attgggccgg aaagactcgg tgtatttgac ttactttttg attgggcaca agacggcaaa
     2521 ggtttcccaa tgatcggaaa tggaaaaaat cgttatcagc ttttggatgt tgaggacctt
     2581 tgtctggcga tctatctaac tgcaacactt gatgaaaaaa ttgtaaatga tacatttaat
     2641 attggcgcaa aagagtttac tactatgagg gaggactatc aggcggtttt agattatgcc
     2701 ggttttggta aaaagattgt cggtcttcct gaaaaaccaa tcatctacac tttaaaattt
     2761 ttagaagcat taaagctttc tccgctttac aaatgggtat atgaaaccgc ttcaaaagat
     2821 tcttttgtat caattgaaaa agcagaaaaa atcttagggt acaaaccaaa gttttcaaac
     2881 aaagatgctc tgctgagaaa ttataaatgg tacgttgagc acagaaacga atttataaac
     2941 aaagacggtg tatcgcatag agttccatgg aaacaaggaa ttttgcgatt tgctaaaatc
     3001 tttttctaat taagtggaat gtgattaaag ttaatcatat ttttttgcaa ctaatccaga
     3061 aagaaaataa atcattaagg agtaaaaaat ggctgagaaa aaagattatc taaaagagat
     3121 cgttaaacat atagatatca agcaacacaa tgttgttcca cttgtagatg ctatggaaga
     3181 tatggctttt acagcaaggg atttaaatcg tgctgcaaat attttagaca caatgataaa
     3241 tgataaagat tgcgcagtta ttttaaccct agcaggaagt ctatttagtg cgggtttaaa
     3301 aaaagttgtt tatgatatga ttatgaacaa catggttgat gcaatcgttt caaccggtgc
     3361 tattatggtt gatcaggatt tctttgaagc actcgggttc aaacattaca aaggaacaaa
     3421 atgggttgat gataatgaaa tgagagaatt acacattgat agaatttacg atacgtttat
     3481 tgatgaagat gaactaagaa tttgtgatga taccactgcg ctaatttttg acagtatgaa
     3541 acccggagca tattcgtcaa gagaattttt gtgggagttt ggaaaatatc tagatgaaaa
     3601 cggcggacca aaagttgatg attctgttgt ttatgcagcc tacaaaaaaa atgttccaat
     3661 ctttattcct gcattctcag attgttctgc tggatttgga tttgtaatgc atcagcataa
     3721 aaatccggat aatcatgttt cttgtgattc cggaaaagat ttcctggaat taacaaaaat
     3781 aaaattacac agcaaagata ccggcatctt gatgattggc ggtggtgttc ctaaaaactt
     3841 tacccaggat attgtagtag ctgcagacat tctacaagag gatgctgaaa tgcataagta
     3901 tgcaatacaa attactgttg cagatgaaag agatggcgca ttatcaggat caactttaaa
     3961 agaagcaagt tcctggggta aagtttccac atcttctgag caaatggtat atgctgaagc
     4021 aaccgtcgct atgcctttaa ttgtgggata cgcttatcat aaaggcggat ggaaaaatag
     4081 aaaagccaga gagtttcaaa agatttattc taaagagaat gcaattgcat aataaactaa
     4141 aaacgcctca agtgaggcgt ttttaattat ctccaattat tcttaggtct cccccaggga
     4201 cctggcacta gaataaccct gggatctatt ttacggagca aatctaaatt ttagttgcac
     4261 tgtaataaat agttaatttg cactatttta gttatgaaaa atgcgatttt gataaatctt
     4321 taattgacat cggacaatta tttgtactga gtacaatatt ttttactttt attaataatc
     4381 tttgaacaat aaataatgtt gaatttgata tgagaattat tattgctgga atgggagatg
     4441 ttgggtatca gttagcaaaa caactttcaa atgaatcaca tgatattatt gcaattgata
     4501 cgaatctaga gcgtctttct tattccgacc agatggctga catacttaca attaaaggtt
     4561 cttccacttc tataaaaata ttaaatgaag caggagtttc aaaggccgat ctgcttgttg
     4621 ctgttacttc atctgaagaa gtaaatattg ccacatcaat aattgcaaaa aaacttggag
     4681 caaaaaaaac tattgcaagg atttcaaatg cggaatattt agatccaacc acaaaaataa
     4741 atttcgtaga aattggtgtg gattttatga tctaccctga agaattggct gcgattgaaa
     4801 ccgtaagctt gataaacaga actgctgcta ctgatgttct agaatttgaa gatggaaaac
     4861 ttgccgttat cgggttaagg cttgataaaa atgcacccat tgcaaataaa aagcttgcag
     4921 aaattgctaa tgaatttcaa aacacagatt tcagagtcgt ggctatccag aggaacttca
     4981 aaactattat accaaaagga aatgataagt ttcttcctaa cgatcaagta tttgtaatta
     5041 caaaaccgga gggacacgat cagatattaa aacttgccgg taaagaaaac atcaagtttg
     5101 ataatattat ggttctcggc ggcggtaaaa ttggcagaca gattgcaagt ttattaagtg
     5161 ataaaatgca ggtaaaatta attgagtcgg atccagataa aactttcgaa cttgctgacc
     5221 aattgccgga tactcttgta attaaaggtg atggaagaga tatcgatctt cttgcacagg
     5281 aaggtattat agatgttgat gcttttattg ccgtaaccga tgatgcagaa accaatatta
     5341 tttcctgttt gcttgcaaaa catcttggtg taaaaaaagt aattgcactt gttgataaag
     5401 tggattacat acctttaact caaactatcg gacttgattc gcttattaat aaaaaattaa
     5461 ttgcagctaa taatatttca cgctttattc ataaaaccga tgttattgtt attgccacgc
     5521 tggaaggtat tgatgcagat gtacttgaat ttaatgttca gccaaattct gcaattacta
     5581 aaaatcaaat ttctgaaatt gattttccta aaggctccat agttggcgga tttataaggg
     5641 gtgatgaagt ttcaatagct accggggatt ccaggattaa tgcgggtgat aaagttgttg
     5701 tattctcctt gccggattca gtaagcaaac ttgaaaaaat ttttaaaaga taaacctgta
     5761 agtgaattat aaaataatta ttaacataat tggttttctc ataatattaa caggcatctt
     5821 tatgatgctt ggacttccat tctctattta ctataaaagt aatgatatac ctgttatatt
     5881 attttccgga attacaacct cttcgattgg ttttacagcc tggttcacaa cccgaaaaac
     5941 tgatagacac gaagtgggta aaagagaagg atacataatt gtaactctcg gctggatttt
     6001 aatgtcggcc tttggagcaa ttccatttgt tattcacggt tctatatcaa gctatactga
     6061 tgcttttttc gaaacagttt caggttttac aaccaccggt gcttcaattc tccccaaagt
     6121 agaagtactg ccatatggat taatgttttg gcgaagcatg actcattgga ttggtggtat
     6181 gggtattata gtactttcac ttgccatatt gcctttgtta ggtattggag gaatgcaact
     6241 ttatcaggca gaggttgcgg gacctacaaa agataaattg catccccggg taagagaaac
     6301 tgcaaaaaga ctgtgggcaa tttatttctt tctaacatct gctgaagtta tacttttact
     6361 gtttggcaac atgaaccttt ttgattcatt atgccactcg tttggtacat tagcaacagg
     6421 cggtttttcg accagaacac agagcattgc atactatcac tcagcctata ttgagtatgt
     6481 aatattgttt tttatgtttt tgggcggcac aaattttacc ttacattatc ttgcgcttca
     6541 tggcaaaatc aattcttatt ttaaagatga cgaattcaaa ttttatttaa gtttaataat
     6601 aattacagta accgcactta ccatttatct tattcttgct gatggtactt cattagaaag
     6661 cacttttcgt aaagttgcgt ttagtgttct ctcaatactg acttctactg gatttgcaac
     6721 tgctgactat gaactttggg caccattttc atcaatgtta tttcttatta ttctgctgct
     6781 tggtgcttgt gcgggttcta caagcggagg agtgaaaatg atcaggtacc agctgctgat
     6841 taaaaacagt attactgaaa taaggaaatt acttcatcct aatgcaattg tacctgttag
     6901 acataatggt agatccgtac ccggagatat tattgcaaag gtttctgcat ttgttcttac
     6961 ttaccttgca atatttgtaa tcggttctct gatgctctca attacaggat tagatctaaa
     7021 aacttctatg ggttctgttg ctgcttgcat ggcaaatatt ggtcccggtt tagggcttac
     7081 aggacctgct ggtaattatt cacaagtttc tgattttggt aaatggttac ttagcgctct
     7141 tatgctgctt ggcagactag aactttttac agttctaata atttttactc cctacttctg
     7201 gaagaaataa ctttcttttc tgataatatt cagttttctt cataatttat tacaaatgtc
     7261 cgtcgtttta gcacggcaaa tcttttgtaa ttagcttata tttgggcccg attggaattt
     7321 aattatatga atacaaaaga actattaaaa aaatacagca agccggaaaa ttttataaac
     7381 agggatttaa gctgggttga atttaataaa cgtgttttgg aagaagcact aaatcctgaa
     7441 ttgcctcttc tagaaaaaat taaattcatt tccattttct gctctaatct tgatgaattt
     7501 tatatgatta gaattgcagg tataaaagaa caaatcgctg caaatgttga agaaccttca
     7561 attgatggat tgactccgat tgaacaattg cgaaaaattg aaaagacttt aaaaccgctt
     7621 ctaaaaaatt tagataatta ttggaccaat tatataattc cctctttaaa ggaaaatagt
     7681 gtcaaacttc tcactatgga tgactttagt gaaggagaaa aaactaaact tagagaatac
     7741 tttaaaagag aaatttatcc ggtacttact ccacttgcat ttgatccggg tagacctttt
     7801 ccatacatat caaacttaag tttaagtctt gcaatattaa ttagaaaacc taatggtgaa
     7861 aatcattttg caagagtaaa agtgccaagt attcttccaa gactgctcca gattgataac
     7921 attattgaac caaataaatc accgggagtt aacggcaatt ttaccgcaac atatatttgg
     7981 cttggcgatc ttatcaaaga aaaccttcct ttactttttc ccggtatgga aatagtagaa
     8041 gcacatcgat ttagaattac acgcgatact gatattgaac ttcaggaaga tgaagctgat
     8101 gacctcttaa gtgtgattga agaaaatatt aagcagagaa gatttggaag tgtagtacga
     8161 ttagaagttg gtcatcctgt gccagatttt atgctggaca cattaattga taatcttcaa
     8221 attaccaaag atgatgttca cgtctctgat ggtcccctgg gtttaagtga tgtaatgagt
     8281 ttattcaaac tgccgcttca tcaattaaaa gaaaagccat tttatccagt tattccaaaa
     8341 gtatttgaag atgaagaaga ttacttttct atcatcaggc aaaaagatat tttgcttcat
     8401 catccttatc attcgttttc accaataata gattttatca aacaagctgc actcgatccg
     8461 gatgtacttg caatcaaaca aactctttac agagtggggc cggattctcc aattgtcaga
     8521 tgtttgattg aagctgctga aatgggcaag caggtagctg tactagtaga attaaaagca
     8581 agatttgatg agcagaacaa catctattgg gcacgtgctc ttgaaaaagt gggtgtgcac
     8641 gttgtttatg gtttggttgg attaaagaca catgcaaaga tgaccttagt agttagaaaa
     8701 gagtatgatg gtgtaaaaag atatgtgcac cttgcaaccg gaaactacaa cgcaactact
     8761 gcaaagctat atactgatct tggtttcttt acttctgatg atgatatatg cagtgatgtt
     8821 tcagatatat ttaatttcct aacaggatat tcgaaacaga aagaatataa aaagttattt
     8881 gtttcacctg ttaatatgcg tgaaagattt attgatttaa ttaatagaga aattgataat
     8941 gctcttgcag gtaaagaagc aaagatcata atgaaattaa attcactggt agatccggct
     9001 attatatctg ctttgtatga agcatccaac tccggcgttg agatcaaatt aattgtaaga
     9061 ggagtttgca gcctaatacc gggagtaaaa aaccttagtg aaaatattga ggttcgtagc
     9121 attgttggaa gatatcttga gcacagcaga ctattttatt tttataatga tggcaccgaa
     9181 gaaatatatt taagcagtgc agatatgatg cagagaaatt tagatagaag agttgaggtt
     9241 gcatttccga ttgagaacca gaaacttaaa gacgaactaa ttaaaacttt aataaaagtt
     9301 gcattaaaag ataatgttaa agcaaggata ttacagcctg atggttctta tattatggcg
     9361 gaagaaaaag aaagtactaa aaaaataagt agccaggaat ggttaatgaa tcatgctcta
     9421 aaggctatgg gagttagatc aaaaaagcca tttggtaaat aatttgaact tatttaccaa
     9481 ataacaattt tagaaatcat tgatgtcatt tagtttattt aaaaagaaac aggaaagtaa
     9541 aatgttcgaa atcacaaaaa gcacagtcat aaattcatta aaaaatatca gtaatgatag
     9601 aataaataca ggattgttca ccttaaattc tattagaaat tttgaaatca ataatgacga
     9661 tttatcatta gagatttctg ttaatgctga taacacagaa gaactaaaag agctaataaa
     9721 aaatcaactt aaaaaagatt ttagcggatt gaaaaatatt aacttaacta tgaagacaaa
     9781 atcatcttca actgtttcaa cacataaaga tgcacgcaaa gatgctatac tgcccggagt
     9841 taaaaacaca attgcaattg caagcggtaa aggcggggtt ggtaaaagca ctgtagcggt
     9901 taatcttgcg gtcgcactcg caaaagatgg cgcaagtgtc ggtttgattg atgcagatat
     9961 ttacggcccg agtattccaa taatgcttgg atcaaaagac aaaccgaagg tttatcaagc
    10021 cgaaaattct gttaaaatga ttccacttga aaatcatggg ataaaattta tgtctatagg
    10081 tcttcttgtt gatgataaag atccgattat ctggcgtgga ccaatggcaa gtggtgctat
    10141 aaaacagttt atgactgatg ttgattgggg tgaattagat tatttgattt ttgatttgcc
    10201 tcccggcaca ggagatattc agcttacatt agttcaaaca attcctttaa ccggagcagt
    10261 aattgtaact actccacagg aagtttcttt aattgatgca cgcaaggcac ttaaaatgtt
    10321 tgagcgggta aatgtgcctg tacttggtgt tgttgagaat atgagttact ttattgcacc
    10381 tgataccgga aacaaatatg atatttttgg ttcaggcggc ggagaaaata tttgcaagga
    10441 tcttaacact acattccttg gtggcctgcc aatcaacccg ggaataagac aaggtggaga
    10501 tagcggcgta cccatggtat ttggtattcc cgattcaaat gaaactaaaa tactgatgga
    10561 tatctcaaga aaacttactg aacaagtaaa tataagaaac tcaaatgctt ctgataaaat
    10621 tgaaattctt cttggggatg aagattaatt ttgatttagt taataattaa aacgatatta
    10681 gtaataaatt tggttctatt tttactaaag attccttaat aatgaaaaac gaagtccaaa
    10741 aagctctaaa caacatcaga ccctacttgc aggctgataa tggtgatgtt gaactaattg
    10801 atgtttcgga tgatggaatc gttaaagtaa aattacttgg ggcttgtgaa atttgtccgt
    10861 tatcaattat gacgttaaga gctggaattg aaagagcaat tatgcgcgaa gttcctgcgg
    10921 taaagagaat tgaagcagta agctgatagt attatggatt tattgaataa actaaatatt
    10981 cggttctttc ttttattatt ggctattgta gtactttttt acttttcata tttacttatt
    11041 aatgtattag tcttaatagc catttcagtg ctgttgggct ttatctttgc accatttgta
    11101 agaactgtcg aggggcaagg attttccagg acaatttcta cgctaattgt ttttattatt
    11161 tttgcttttt tattatatct tggcctttca tttgtgatac ctaaattcta ctctcagatg
    11221 gatcaggtta taatatcttt aaaggatttt tctttaaatg aagaacttaa cgatctggaa
    11281 aaaaagattc taagcgtctt ccctctcttt aataaaggtg aactttcgca aaaagtacaa
    11341 agtattattt caacctcatt caatgattca ataaatcata taacagagta tgtaagcagc
    11401 gttgtttctg ttgctgcctt tttagtgatt gttccattca taacttttta cctgctaaaa
    11461 gatagtgctg ttatacaaaa aggattgatt cacattgttc ccaataaata ttttgaaatg
    11521 tcttactgga tattaaagag aataagtctt caacttggca gatttgttag aggttggatt
    11581 tttgatgcaa catttgttgg cattgccata ggttttggat tttattttat tgggttgcca
    11641 aacgcccttc cattaggagt aattgctggc atcgggcacc ttgttcctta ccttggacct
    11701 gtaataggcg gaatccctgc aatagtacta tccataatac aaacagggga tttatctcag
    11761 gttccactaa taataatgat agttgcttta acttacacat tggacaacgg atttgttcaa
    11821 ccttatgtct tttctaaaag tgtggatatg catccaataa ttattatact tctaattatt
    11881 atcggtagtg aattgtttgg gataatagga atgttactag cagtgccaac tgcaacagta
    11941 attaaaactg ccgcaaaaga aatttatttt gcttataaaa attactctat tgctaaaata
    12001 taaatagctt ttagttacgc ttctgtttca actctattaa tcacatcaat agaagatatt
    12061 ccctcagctt cagcactaaa atttgttata attctatgtc gtaaaacagg cagcatggag
    12121 gcttttatat catctatatt tggtgtaaat ctgccctgaa taatagaacg tgtttttgcg
    12181 gccataacta agtactgtga tgctcttggc ccggctcccc aggtaaccca atccttaaca
    12241 tacttgggtg catttccgtt cattggtctt gtcatattag acactttaac agcaaactca
    12301 atcacatttg gtgcaactgg tacttttcta accagatcct gaaacattat taattcatct
    12361 gaagaaacaa ctttttttaa tgttgcttta tattcacttg tagttgtttg tactaccttt
    12421 atttcttctt caacagaagg ataatcaagc caaagattga acataaaccg atccagttgt
    12481 gcttcaggaa gaggatatgt tccttcttgt tcaattggat tttgagttgc aagaacaaaa
    12541 aatggttctg gtaattgata agtctgaccg gcggcagtta ctttatgttc ctgcattgct
    12601 tctaataatg ccgcctgagt tttaggcgga gttctgttta tctcatcagc aagaataata
    12661 tttgcgaaaa ttggacctga gataaaccta aaatttcttt ttttagttgc tagattttct
    12721 tcaagtattt ctgttccggt aatatcgctg ggcataagat cgggtgtaaa ttgtattctg
    12781 ctgaacttta gatccatcac ttctgcaaga gttttaatca gaagcgtctt tgctaaacca
    12841 ggtacgccaa ccaataaaca gtgtccacga gacaaaagcg aaattaagag ttgattgata
    12901 atctcatcct gcccaacaat tactttggca atttctgatt ttatttctgt tactttttta
    12961 tttagctgtt caaccaattg aacatcatta tttactgaac ttaattcgtt cacttaatat
    13021 cctataaatt taaattctaa cttcccaata aattttactt tttatctctt caatccactt
    13081 tgtgtattct aattgtttct tgtgttcatc agctagtttt tttatttcat cataatcatc
    13141 tgttaagttt gctttatgct ggggaaccct tgatttaagc caaacgatat gatatccata
    13201 ggtgcccggc gcatactcta atcttcttgg aaagccaata tcaccttcct taagtttacc
    13261 aacagcgtcc agtaatgctt tatctaattg gctgatataa aaattaccaa gctcgccgcc
    13321 aaagggcgct gtttctttgt cctcgctata tttttttgca aattcctgga atgttccttt
    13381 gccctgcaga atgctgtctc tgatagcaga aaggaaatca attgtttgca gatctgcatt
    13441 ttcatcagcc ttaatcttta taaggatatg tcttgtatga attgattctc ctcttttttc
    13501 aatcatttgt atgatgtgaa aaccaacagg agattctaca acacctgata attcaccaac
    13561 atttaattta aatgccgcct cttcaaactc gggatagaac acaccttttt ttacaaaacc
    13621 cagatcaccg ccttgagcag cgctgcctgg gtcatcagaa tatttttttg caagttcggc
    13681 aaaatcttta ccggctttta atgaatccaa aagtgcgaga gctttatcat aaaatttctt
    13741 ttttaatttt tcgctggctt taggattctg gaagatatga aagattgtta ctttttccgg
    13801 aatattgcca atgctgtctt tataagtact aaaaaaatct tctacttctc ttctggttgc
    13861 ctgaactttg ccaaagtttt tttcttgaag tcgttgagac atcaagcttt tacgaacttc
    13921 atcacgtaat tctcttttaa tcctgtctat actcatccca taaatttttt ctatgttagc
    13981 aacagagcca tattgctgag taaaagagtt tatttgataa tcaattcgtt gatttatttc
    14041 ttcttcagtt acggtaatcg aatcaagatc agcttgagca taaataagtt tttcctcaat
    14101 catcgtattt aaaagctgtt cttttaaacc cggtgtattt ggatcaattt gtctttgtga
    14161 agcaaaaatt ccaacttgaa aatcaagttc actttgcaga ataatttcat tatcaacaac
    14221 agcaactatt ttatcaagta ctttttgggg tgatataact ccggcaaata ataaaatcaa
    14281 tgcagcaatt tttattttca tttttttatc tcaatttcat tacgggaata aaggtctcta
    14341 agatactctt ccactaatag tttcttcttc tcagctaaaa atcttttttc aaccgttggt
    14401 ttaataattt caaaaggtgg aatagattgt ttgccatatt tacctaacaa ttgcaccacg
    14461 gaataatatc caggtttttc tgtaattaca atactaattt cttccggata aaaatccttc
    14521 acaattctag ataactgcat agggtagata ttactttctt ccactaaatc tgataaaaca
    14581 tttttgacta gagaagaatc gtttacaaaa acacttactg cttttttcca ttcactttct
    14641 aaagcaagcg ttctgaattt tatagcatta tcttcactgt taaatgtaac tttgtttatc
    14701 aagtaagaat cggagttcaa tcggaaataa tttttgctag actcataata attgttaata
    14761 tcatcatcag aatatttaat ttcttcatta gccagataat catcaattaa catagaagcg
    14821 gccaattcat ttgatgattt tttaattata ctcaaataat ctttgcgttt agttattcct
    14881 tctttatcgg cagcttgaaa taaaatttcc tggtatatcc aatttttaat cagttgttcc
    14941 ttttgtgcag aattcagatt agaagtatct gccagggaag caaa
//
LOCUS       DASWBY010000034         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_39133, whole genome shotgun sequence.
ACCESSION   DASWBY010000034 DASWBY010000000
VERSION     DASWBY010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39133"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..538
                     /locus_tag="VF870_00680"
     CDS             47..538
                     /locus_tag="VF870_00680"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9250719.1"
                     /translation="MEQIFNTIQKAGTFLFLLLVIFTMGCQQPQEAKPETPEYFYLRP
                     EVEKTYGYTHAVKIGNDIKISGAVSMDDAGNPTAVGDLLQQMKNCYSDLDKVLKHYGC
                     TFDDVVVENVFTTNMPEFLNQAGYRNTIYKKQFPTGSWLGVKELALPEFMIEIELEAH
                     KAN"
     gene            638..1936
                     /locus_tag="VF870_00685"
     CDS             638..1936
                     /locus_tag="VF870_00685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9250720.1"
                     /translation="MSENTFLRYFSFAALYVAQGIPEGLLWYAIPAWLAINGKTPGEI
                     GSYVAVIGIPWSLKIINAPIMDRFTYLSMGRRRPWILFGQFGLIASFLSMSLIIDPLN
                     NLSALMILGFIVSFFEVSQDIAVDGMAIDILPVDQQARANGLMWGSKTLGISASVAAG
                     SWIMSHYGYFFAIVSFSLVISIIILVPLLLRERPGEKLLPWTDGEASKFAEKIQLHSW
                     KGIFKSLYKVFFLPVSFMMGVAAFSYSVGRGLIDTVLPVFTVQQLGWADTQYSQIFAT
                     ANLISGLLGMVIGGFLIDYFGKVKMMSVYLSLLILIAAAMSFLKSFWQNNIFVTGFII
                     CFYILLTFTNIAIFASAMQLCWKRVSATQFTLYMAISNLGLAAGAALLGPLKDLFNWE
                     YVILSYIVFALGMLVLIRFINFDKHQLRVEILEEKYLEQN"
     gene            2036..2680
                     /locus_tag="VF870_00690"
     CDS             2036..2680
                     /locus_tag="VF870_00690"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9250721.1"
                     /translation="MFKSLVIAGLCLCLCSNIFSQGFSLPSKGNQVFDFKDSQERNQA
                     TFLSEARYENITGLTNDVWGKVSFEAKDIKSTLAGEITISASSIKTGIEKRDEHLQSV
                     SWLNSEKYPTITFKIKEVKRVELVEDNELKIVVLGEFNLRGKTNLVYANATMKYTPEN
                     DVTKTIMQGNLLSIIANFEINLSDFGITNSLIDNRVSDKIKITANLLGSDSYQK"
     gene            complement(2640..>2846)
                     /locus_tag="VF870_00695"
     CDS             complement(2640..>2846)
                     /locus_tag="VF870_00695"
                     /inference="COORDINATES: protein motif:HMM:NF027338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEX9250722.1"
                     /translation="HIKVLENISKMKFKCDDAIGNFYKCGDETEFKHYGMWSLIENST
                     PDFHLPEYFGKIIFGKNQNLEDLL"
BASE COUNT          913 a          421 c          542 g          970 t
ORIGIN      
        1 agtataatca aaacatattt atcattatta tcagggagat gctgaaatgg aacaaatatt
       61 taacaccata caaaaagctg gaactttttt atttctcttg ctcgtcatat tcacaatggg
      121 atgtcagcaa ccgcaggaag caaaacctga aacacctgaa tatttttatc tccgtccgga
      181 agttgaaaaa acttacggtt atacacacgc tgttaagatt ggtaatgata ttaaaatatc
      241 cggcgcagtc agcatggatg atgcaggcaa tccaactgct gttggtgatt tattacagca
      301 gatgaaaaac tgttattcag atttagataa agtattaaaa cattacggct gtacttttga
      361 tgatgttgtt gtagaaaatg tttttactac aaatatgcca gaattcctaa atcaagctgg
      421 gtatagaaat accatttaca aaaagcaatt tccgacaggt tcgtggttag gcgttaaaga
      481 attagccttg cctgagttta tgatagagat tgaattggaa gctcataagg caaactgaat
      541 attatattaa aaaattattc gatacttaga aacagaggag aatcagctag tgctttttgt
      601 agaatctgaa aaaattaaat caaagccact tccctctttg tcagaaaata catttttaag
      661 gtacttctca tttgcagcat tatatgttgc acaaggaatt ccagaaggat tattgtggta
      721 tgcaattcct gcatggctgg caatcaacgg aaagactcca ggcgaaattg gaagttatgt
      781 tgcggtaata ggaataccat ggagtttaaa aataattaac gctcccatta tggatagatt
      841 tacatattta tcaatgggtc gaagaagacc atggatttta tttggtcagt ttggattgat
      901 agcaagtttt ttgtcaatgt cactaataat tgatccgctt aataacctat ctgctctaat
      961 gattttggga tttatagtaa gcttttttga agtatcacag gatattgctg tagatggaat
     1021 ggcaattgat attttgccgg ttgatcagca ggcaagagca aacgggttaa tgtggggttc
     1081 aaagacatta gggatttccg cctcagtagc tgcgggtagc tggataatga gtcattatgg
     1141 atacttcttt gcaattgttt cattctcatt agttatttct ataattattc ttgttccttt
     1201 attacttaga gaacgaccag gagaaaaatt gcttccctgg acagacggag aagcctcaaa
     1261 attcgcagag aaaattcaat tacacagttg gaaaggtatc tttaaaagtt tatacaaggt
     1321 tttctttctg cctgtaagtt ttatgatggg tgttgctgct tttagctatt cagttggtag
     1381 agggttaata gatactgtat tgcctgtatt tacagtacag cagctaggct gggctgatac
     1441 gcaatattca caaatctttg cgaccgcaaa tttaatttca gggctacttg ggatggttat
     1501 cggcggtttt ctgatagatt attttggtaa agttaaaatg atgtctgttt atttgagtct
     1561 gctaatttta attgcggctg caatgtcttt cttaaaaagt ttttggcaga ataacatttt
     1621 tgtaacgggt ttcataatct gtttttatat ccttttaact tttacaaata ttgcaatttt
     1681 tgcttctgca atgcagcttt gctggaagcg ggtttccgca actcagttta ctctttatat
     1741 ggcaatttca aatcttggtc ttgcagcagg tgccgcattg ctcggccctt tgaaagattt
     1801 atttaactgg gagtatgtaa ttctgagcta tatagttttc gcgttgggaa tgttagtgct
     1861 tattcgtttc ataaattttg ataaacatca attgcgtgtt gagatattag aagaaaaata
     1921 tctggagcag aattgattta taatattcaa actttaatat tcttttagta gttatttttt
     1981 tatttattaa attagaatat gctgtttcaa tcaaataaac gtaaaaaggg atgttatgtt
     2041 taaatcatta gtaattgctg gtttgtgttt atgtttatgt tcaaatatct tttctcaagg
     2101 ttttagttta ccttcaaagg gcaatcaggt ttttgatttc aaagatagtc aggaaagaaa
     2161 ccaggccaca tttcttagtg aagctcgtta tgaaaacatt accggattga cgaatgatgt
     2221 atggggcaaa gtatcttttg aagcgaaaga tattaaatcc actttagccg gggaaataac
     2281 aatttcagcg tcatcaatta aaaccggcat agaaaaaaga gatgagcatt tacaaagtgt
     2341 ttcctggctt aactcagaaa agtaccctac tattacattc aaaattaaag aagtgaagcg
     2401 agttgaatta gtagaagata atgaattaaa aattgttgta ttaggagaat ttaatcttcg
     2461 cggaaaaact aatcttgttt atgcaaatgc aactatgaag tataccccgg aaaacgatgt
     2521 aacaaaaaca attatgcaag gtaatctctt aagtattata gctaattttg agatcaatct
     2581 ttctgatttt ggaataacca attcattgat tgataacagg gttagtgata aaataaaaat
     2641 tacagcaaat cttctaggtt ctgattctta ccaaaaataa tcttcccgaa atattcaggg
     2701 agatgaaaat ctggtgtgga gttttctatt aaactccaca taccgtagtg tttaaattca
     2761 gtctcgtcac cgcatttata aaaattgcca attgcatcat cacatttaaa cttcatttta
     2821 gaaatatttt ccaatacttt gatgtg
//
LOCUS       DASWBY010000035         5213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_1382, whole genome shotgun sequence.
ACCESSION   DASWBY010000035 DASWBY010000000
VERSION     DASWBY010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2409)
                     /locus_tag="VF870_00700"
     CDS             complement(<1..2409)
                     /locus_tag="VF870_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9250723.1"
                     /translation="MANLKPLKHKELKPAQLRWKCDPNIFSFKSTEDIDPIEGILGQE
                     RALKAIRMGVDLRSPGYNIYIAGLSGSGKATTVKKMLEKISADCPRLYDYAYVNNFRD
                     PDQPLLIKFPKGQAREFKQDLNAAIEVLKQRIPIALESDLYLSRKKNIVDEYNQKEQE
                     LMNSFDKELRKKGFSLGQIRVGEQIRPDIIPLIDGQPVPVFQLEELIKQNKLTKEDAQ
                     QIILDYNGHQPKLQIIFKKGLKISQEFQEKLQQLEKESAEVIVRGIFEGLKEKYNSNS
                     ALEHLNLVEDNILDNIQIFKGIKPEGEMTQEGMMIDYFRDYDLNIILDNSETVECPVI
                     VETNPTYINLFGTIEKVNDGKGNWYSDFTNIKSGSILRANGGYLVLNVMHLFEEPGVW
                     KSLKRILTYCKLEIQESPYHFSMSSTSLKPEPIDIDTKVILIGSQMVYAYLSEREYDF
                     KKMFKVKADFDYEIIRNNKVVIEYAQVIKKLIKDEKLKEFDKSAIAYLLEISAILAGR
                     QNKLTLRFSQIADIAREASFWAADDGFNVVSAAHIEKAYQLARERHGMLEDKVTEMFE
                     NKTFLIDTDGERVGQINGLAVYNADFYSFGRPTRITATVSLGSGTIINVEREAGMSGR
                     HYNKGMLIISGYFRETFGQDQPLSFNANLVFEQSYGMVDGDSASCTEIFALLSTLSGL
                     PIKQSIAVTGSLNQKGDVQPIGGVNEKIEGFFDICKLNGLTGNQGVIIPIQNIDDLML
                     RDDIIEAVRKNIFHIYPIKRVEEGIEILTGTKAGKKSAKGYEEGTVFYRVEKKIQELF
                     EKSRA"
     gene            2501..3628
                     /locus_tag="VF870_00705"
     CDS             2501..3628
                     /locus_tag="VF870_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9250724.1"
                     /translation="MNKTEVRNNFPYLDNGIIYFNHASTGPISSLVVNRLTSLLKEKS
                     ENKIDDYPSFLKVVEETKILLAELINCNIDRTAFIDNTSNGLNILAQSIQWKKGDRIL
                     LNDIEFPANVYPFLNLKRFGVEVDFVKSENGIVTADQIINAIKPQTKLVSVSFVQFLS
                     GYKVDIEKIGNYCRSNNIIFCVDGIQGIGAVTIDVKKCKIDFLSCGTQKWLLGMQGLA
                     FVYIDEELQRKIIPANVGWLSVENAWSLLDYKLDLKTSANVFQGGTLNAFGIYIFNTS
                     LKLFKDFGFENIELEVLSNTKYFISKLKSVGLDCSLSNCSDAELAGIVTIKPRDAANV
                     FEELEKKKIFCSIREGFIRFAPHFYNTHHEIDKVVEELQKI"
     gene            3661..4203
                     /locus_tag="VF870_00710"
     CDS             3661..4203
                     /locus_tag="VF870_00710"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9250725.1"
                     /translation="MKRFYSIVILLFAFAFITSSTQAQNNKKDLRISPKAEVMQVVGF
                     TEVRITYSRPGVKGRTIWGKLVPYDAVWRAGANEATKITFSTDVTIEGKKLKKGSYSF
                     FAIPGKNDWTLIFNKVADQWGAFEYNESEDALRVKVKTEKAVWQEWLSYTINKASDTS
                     AVIRLEWEKIKVPFKVEVKL"
     gene            complement(4207..>5213)
                     /locus_tag="VF870_00715"
     CDS             complement(4207..>5213)
                     /locus_tag="VF870_00715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9250726.1"
                     /translation="FFFLFYALWAKIFGSSIENLRIITLIISFITWQFVYYLFQLFVK
                     KESHALLLSFLVVANPYFFGTSVFVFTDMLTILLSLAAIISFRINKLLQFIIFSALAI
                     LCRQYAVIIPAVVIIYSLLNYKNDRLNNRNYVIGSFLTFLPLVILFGVWKNIAPASGI
                     EKWVVPNSSFYNIDYINTYITFSVIYLFPLAIIFLKKIKLSYSSLFIAFLLTVILSFF
                     PIKSSIATLDFTDYKSVGFVHQGLINVFGYESIVLKIVLGIFLLAGCYISAELMKRFY
                     LHLKIKSFDLSIISTLLWVLFLLIMPFSYQVWEKYLTMILPFLILSIYLVLYPVEANK
                     "
BASE COUNT         1747 a          855 c          845 g         1766 t
ORIGIN      
        1 tgcacgagat ttttcaaaga gctcttgtat tttcttttca actctataaa aaacagttcc
       61 ttcttcgtaa ccttttgctg attttttccc agcttttgtt ccagtaagaa tttcaatccc
      121 ttcttctact cgttttatcg gatatatatg aaatatattt tttctaacag cttcaataat
      181 atcatcacga agcataagat catcaatatt ctggatagga attattactc cttgatttcc
      241 agttagtcca ttcaacttac aaatatcaaa aaagccttct atcttttcgt taacaccgcc
      301 aattggctga acatcacctt tttgatttaa tgatcctgta actgcaattg attgttttat
      361 tggtaatcct gatagtgtag atagaagagc aaaaatttct gtgcaggatg cgctgtctcc
      421 atcaaccatt ccgtaagatt gttcaaaaac aagattagca ttaaaagata atggttgatc
      481 ttgtccgaat gtttctctga aatagcctga aattattagc atgcccttat tatagtgcct
      541 gccgctcatt cctgcctcgc gttctacatt aataattgta ccgcttccaa gagaaacagt
      601 agctgtaatt cgtgttggcc ttccgaagga ataaaagtcg gcgttataaa cagcaagtcc
      661 atttatttgt ccaactcgct caccatctgt atcaattaaa aaagttttgt tctcaaacat
      721 ctcagttact ttatcctcaa gcattccgtg tctttcacga gcaagttgat aagctttttc
      781 tatatgagct gccgatacga cattaaatcc gtcatcagcg gcccaaaaac ttgcttcgcg
      841 cgcaatatct gctatttgtg aaaaacgcaa cgtaagtttg ttctgtcgcc ctgccagtat
      901 tgcgcttatt tctaaaagat atgctatcgc agatttatcg aactccttta atttttcatc
      961 tttgattaat tttttgatta cttgtgcgta ctcaattaca actttgttat tacgaataat
     1021 ttcataatca aaatcggctt ttactttaaa cattttctta aaatcatatt cgcgttcact
     1081 tagatatgca taaaccatct gactgccgat taaaataact tttgtatcaa tatcaatcgg
     1141 ttcaggtttt aatgaagttg atgacataga aaaatgatat ggtgattcct gaatttctaa
     1201 tttgcaataa gtgagtattc tttttaagga tttccatact cccggttctt caaacaaatg
     1261 catcacatta agaactaaat atccgccatt tgcacgcaga atagaaccgg attttatatt
     1321 tgtaaaatca ctgtaccaat tacccttgcc atcatttact ttttcaatcg tgccgaataa
     1381 atttatatat gttggatttg tttcaacgat aactggacat tctactgttt cagaattatc
     1441 aagaataata tttaaatcgt aatcgcggaa gtaatcaatc atcattcctt cttgagtcat
     1501 ttcaccttct ggtttgatac ctttgaatat ttggatgtta tccagaatat tatcttctac
     1561 aagattaaga tgttctaaag ctgaattgga attatatttt tcctttaagc cttcaaaaat
     1621 tccacgtaca attacttctg cactttcttt ttcgagctgc tgtaattttt cctgaaattc
     1681 ctggctgatc tttaatcctt ttttaaatat gatctgcagt tttggctgat gcccattata
     1741 atcaagaata atttgctgcg catcttcttt tgttaactta ttttgtttga taagttcttc
     1801 gagctgaaaa acaggaaccg gctgtccatc aatcaatgga ataatatccg gtcttatctg
     1861 ttcaccaact cttatctgtc caagtgaaaa acctttcttt cttaattctt tatcaaaaga
     1921 attcattaac tcctgttctt tttgattata ctcatcaaca atattttttt tgcgggaaag
     1981 gtaaaggtca ctttcaagtg cgatgggaat tctttgcttt aaaacttcga ttgctgcatt
     2041 caaatcttgt ttaaattctc ttgcttgtcc tttaggaaat ttaattaaca gcggctgatc
     2101 aggatcgcgg aaattattta cataggcgta atcataaagt ctgggacaat ctgcacttat
     2161 tttctcaagc attttcttta cggttgtagc tttacctgaa ccagaaagac cagctatata
     2221 aatattataa cccgggctgc gtagatcgac acccattctt attgctttta atgcgcgctc
     2281 ctgtcctaat attccttcga taggatctat atcctcagta gatttaaatg aaaaaatatt
     2341 tggatcacat ttccatctta attgagcagg ttttagttct ttatgtttga gaggttttag
     2401 gtttgccatt ttttacctat ttatatttga tagtagaaga gatataattc attcttattt
     2461 ttgtattgaa aactaattaa actataagcg aatttcaaag atgaacaaaa cagaagtacg
     2521 taataatttt ccttatctgg ataatggaat tatatatttt aatcacgctt caacgggtcc
     2581 aatctcatca cttgttgtaa atcgtttaac aagtcttttg aaagaaaaaa gcgaaaacaa
     2641 aattgatgat tatccatctt ttcttaaagt tgtagaagaa actaaaattc ttctagcaga
     2701 acttatcaat tgcaatattg atcgtactgc ttttattgat aacacatcaa acggattaaa
     2761 catacttgcg caaagtatcc agtggaagaa aggcgataga atattattaa acgacattga
     2821 gtttcctgca aatgtttatc cgtttcttaa tcttaaaaga tttggagttg aagtcgattt
     2881 tgtaaaatct gaaaatggaa tcgtaactgc tgaccaaatt attaatgcaa taaaacccca
     2941 aacaaaatta gtttccgtaa gttttgttca gttcctttca ggatataaag ttgatattga
     3001 gaaaatcgga aattactgca gatcaaataa tattattttt tgtgttgatg gaatacaggg
     3061 tatcggggca gtgactattg atgttaaaaa gtgcaaaata gattttcttt cctgcggcac
     3121 tcaaaagtgg ctgctgggta tgcagggttt ggcctttgtt tatattgacg aagaactgca
     3181 aaggaaaata attcccgcaa atgttggatg gctttccgtt gaaaatgcct ggagccttct
     3241 ggattataaa ttggatttaa aaacctctgc taatgttttt cagggtggaa ccttaaatgc
     3301 ttttggtatt tatatattta atacttcatt aaaattattt aaagattttg gttttgagaa
     3361 tatcgaatta gaggttttat caaatacaaa gtattttata agtaagctta aaagtgttgg
     3421 tttagattgt agcctgtcaa attgttctga tgctgaatta gcagggatcg taactatcaa
     3481 accgagagat gctgctaacg tattcgagga attagaaaag aaaaagattt tttgttcaat
     3541 tcgtgaagga tttataagat ttgcaccgca tttctacaac acacatcacg agattgataa
     3601 agtagttgag gaattacaaa aaatttgaat atctttaaat aaaaaaatta ggatcggaat
     3661 atgaaaaggt tttatagtat tgtaattctg ttgtttgcat ttgcttttat tacaagttca
     3721 actcaggcac agaataataa aaaagattta cgcataagtc ctaaagcgga agtaatgcaa
     3781 gtagttggtt ttactgaagt tcgtattact tacagcagac caggagtaaa aggaagaacc
     3841 atttggggta aacttgttcc atatgatgca gtttggcgcg caggtgcaaa tgaagcaaca
     3901 aaaatcactt tctcaactga tgtaacaatt gaagggaaaa aattaaaaaa aggttcctac
     3961 agcttttttg ctattcccgg aaaaaatgat tggacattga tattcaacaa agttgcagat
     4021 cagtggggtg catttgagta taatgaatcc gaagacgcac taagagttaa agtaaaaacg
     4081 gaaaaagcag tctggcagga atggttatct tatacaatta ataaagcatc ggatacttct
     4141 gcagttatta gacttgagtg ggagaaaatt aaagttccat ttaaggttga agttaaattg
     4201 tagcagttat ttattcgctt ctacgggata tagaaccaga taaatactca gaattaagaa
     4261 gggaagaatc attgttagat atttttccca cacctgataa gaaaacggca ttattaacag
     4321 gaataaaacc caaagcaaag tagatattat gctcagatcg aatgatttta tttttagatg
     4381 taaataaaat cttttcatca attctgcact aatgtaacac cctgccaaaa gaaaaatccc
     4441 caaaacaatt tttaatacaa tactctcata tccaaataca tttattaatc cttgatgaac
     4501 aaatcccacg gatttgtaat cagtaaaatc taaagttgca attgatgatt taatcggaaa
     4561 aaaagacagg ataactgtaa gtaagaatgc aataaaaaga cttgagtaac tgagctttat
     4621 cttctttaaa aatataattg ctaatgggaa aagataaata actgaaaatg tgatgtaagt
     4681 gtttatgtaa tcaatattgt agaaagatga atttggaacg acccactttt caattccact
     4741 tgcaggcgca atgttcttcc acactccaaa taaaattact aaaggtaaaa aagttaaaaa
     4801 cgaaccaata acataattcc tgttatttag tctatcattt ttataattca gcaaagaata
     4861 aattatcacc acggctggaa taataactgc atattgtctg cacagtattg ccaatgcaga
     4921 aaaaatgatg aactgtaata atttatttat tctaaatgat atgattgcag caagcgatag
     4981 taaaattgtc agcatatcag taaaaacaaa aacactggtt ccaaagaaat aaggattggc
     5041 aactactaaa aatgaaagta aaagcgcatg agattctttc ttaacaaata attgaaacag
     5101 gtaatacaca aattgccatg ttatgaaaga aattataagt gtaattattc ttaggttctc
     5161 gattgaagag ccaaatattt ttgcccaaag cgcataaaac aaaaagaaaa agg
//
LOCUS       DASWBY010000036         7040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_715945, whole genome shotgun sequence.
ACCESSION   DASWBY010000036 DASWBY010000000
VERSION     DASWBY010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715945"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VF870_00720"
     CDS             complement(<1..1039)
                     /locus_tag="VF870_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX9250727.1"
                     /translation="MLLKNKLILSIIFELVFLPSVLFGQEGFRTEKDLLGEKQIPNDA
                     YYGVQTLRALENFQISGVTTKDYPELVNAFVLVKLAAARANYDSGALPKYVLDAVEKA
                     CTEVLAGKYHDQFLVDLYQGGAGTSANMNVNEVLANIALELSGKQKGDYAYIEPHDHL
                     NMSQSTNDSYPTSLHVAIILMNQKLLPELEALIKSFEAKGKEFENILKMGRTELQDAV
                     PMTLGQEFNAFASQLKASIDELKQAESYLYVINMGGTAIGTKLTAQKGFVENCAKHLG
                     ELLKKKIVPAKDLIAATSDLSGFVNYSSALNTLSVRLSKIASDLILLTSGPRTGLFEI
                     NLPALQPGSSIM"
     gene            complement(1058..2389)
                     /locus_tag="VF870_00725"
     CDS             complement(1058..2389)
                     /locus_tag="VF870_00725"
                     /inference="COORDINATES: protein motif:HMM:NF006927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HEX9250728.1"
                     /translation="MMWIELLILFTCIVIGARLGGIGLGTMGGLGLVIFVFLFGLPPG
                     SPPGIVLGMIIAVITALAAMQSAGGLDYLISLATKVMRKKPQYITFVAPFVTYFLILA
                     SGTQHVIYALLPVIAEVSTKAGIRPERPLSISVTASMYGVISSPISAATVALLGALVS
                     YNVSLLQIMMVIIPASIFGLFVGTLSVAWRGKDLKDDTEYQERLKSGKIQESNVAVEM
                     AKEKIGNARNSLLIFLVSIVIVVFIGIFPELRPSYEILSGKGSVIEKLEMGDAIMIIM
                     LAAAGIMMIFFKAKPSEAVKGSIMTGGVVAVISILGVSWMGSSFFEGNKTEIVGGISG
                     VIQQAPWVLALGLFVLSMLLFSQAATVVTLMPVGVALGMPLWVLIAVYPAVNGFFFLP
                     TYGTVLAAVSFDRTGTTKIGKYLLNHSFMLPGVVCVIATTLFAFIISSFVL"
     gene            complement(2440..3489)
                     /locus_tag="VF870_00730"
     CDS             complement(2440..3489)
                     /locus_tag="VF870_00730"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HEX9250729.1"
                     /translation="MKRLLSFFIVLAVAFTLNAQKLPNIVILATGGTIAGAAPTEVQA
                     GYQSGQVGVDILINAVPQLKEIANVTGEQISNIGSQNMSDQVWLKLAKRCNELLAKDD
                     VDGIVITHGTDTMEETAYFLNLVIKSKKPVVLTCSMRPSTALSADGPLNIYNAVAVAG
                     SKESWDRGVMVVANDLIHSGRAAIKGNTTAVETFFSPLVGPLGTVNYGEINYVDVPDK
                     KHTYQTEFNVDNLTSLPRVDVIAIYEDAPGDLIKAAVDFGAKGIVTSGLGNGNLTDAC
                     VEALKYARSKGVVVVRGSRVITGFVGRNVELDDDALGFDASYELIPSKARVLLRLALL
                     KTNDPVKIQEYYETY"
     gene            complement(3525..5141)
                     /locus_tag="VF870_00735"
     CDS             complement(3525..5141)
                     /locus_tag="VF870_00735"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX9250730.1"
                     /translation="MQELKAYSLLYNPRKNKGTAFTKQERLEYGLNGILPDVIESMET
                     QIMRVQEQIENLNKPINKYIYLTGLLDTNETLFFKTIISDPAKYLPLVYTPTVGEACQ
                     RLGHIARRPRGLFISIKHKNKIESILRNWPVEDIRFIVVTDGQRILGLGDLGVCGIGI
                     PIGKLALYTSCAGVPPEHTLPIVLDAGTDNEKFLNDPLYPGIKQKRISGKKYDEFIHA
                     FVKAIEKVYPKVCIQWEDFAGVNAIRILDKYRDKICTFNDDIQGTAAIAVAGFIAVSR
                     LLNKPFKEQKFLFLGAGAAAFGIAEMLVKKFQRDGLSKEESYNRCWMFDVNGLLVKSR
                     TDIAEYQKEFAHDSEPSIDFAGSISKIKPTAIIGVSTVGGAFTQQVIENMSAINDRPI
                     IFPYSNPTSHSECTAEQAYTWSKGKAIFASGSPFEPVTYNGKTFTPGQGNNVFIFPAL
                     GLAIYATEAKRVTDEMLLTASEAVAEQITEEDFNNGLIYPPIKNIREVSVNVAVKIAE
                     EIFKSGLARVKRPKNIRKFIKSKMYEPIYK"
     gene            complement(5191..6873)
                     /gene="aspT"
                     /locus_tag="VF870_00740"
     CDS             complement(5191..6873)
                     /gene="aspT"
                     /locus_tag="VF870_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0070906 - aspartate:alanine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HEX9250731.1"
                     /translation="MNNFIEILQQHPELALFLVLALGFAIGKISIGNFKVGAVLGTLF
                     AGVLIGQFHIEIAPVVKVIFFDLFLFATGYKVGPQFFRGLKKDAVPQLILTLVICVTC
                     LITAFVMSKLMSFDVGTAAGMLAGAFTESTVIGTASESIQRLQIPDAEKTILLNNIPV
                     AYAVTYLVGTTFLVWFLSSLAPKLLKIDLRSASKELEKKLLGKSEDEEGIASAFEDWR
                     LRAFKITNDKWFGMSVQDIQSISTTSRIFIHRIRRNGKIIEPNAGTILQSGDIIAVMA
                     RYNVLFQKFYDIGPEVMDRELLDFPIAYRDLIITNKKLAGKSLSEIGTKYGQGVKLHK
                     LIRTGQEIPFSAETIINRGDTLKISGLLPDVERAAKLIGYIDRVTAETDMIFVGLGIL
                     LGGLVGLLAVTIGGLSISLTTSGGALVMGLIFGWLRSKTPKFGRIPEAALWIFDNVGL
                     ATFIGIVGLSAGPSFISGLSQTGIGLLFAGLIVAITPHIVGLLFGHYVLKINPVILLG
                     AQSGAGTTTAGLKALQDAAGSKLPVLGYTVPYALGNILLTAWGPVIVAMMNF"
BASE COUNT         2185 a         1460 c         1161 g         2234 t
ORIGIN      
        1 gcataattga agaacctggt tgtaatgccg gtagatttat ttcaaacaaa ccggttcttg
       61 gacctgaagt taataaaatt aaatcgctcg caatttttga aagtctaact gaaagtgtat
      121 ttaaagcact ggagtaattg acaaagcctg aaagatcaga tgttgctgca atcaaatctt
      181 ttgctggaac gattttcttt ttcagaagtt ctccaagatg ttttgcacaa ttctcgacaa
      241 atcccttttg tgcagtgagt ttggttccaa ttgctgttcc acccatatta atcacataaa
      301 gataagattc agcttgtttg agttcatcaa tagaagcttt aagctgagaa gcgaatgcat
      361 tgaattcttg tccgagtgtc attggaacag catcctgtaa ttcagttctt cccatcttca
      421 aaatattttc aaactctttt ccctttgctt caaatgattt aattaatgct tcaagttcag
      481 gaagcagttt ttgattcata agaataatcg caacatgaag cgaagtcgga taagaatcat
      541 tcgttgattg agacatattt agatgatcat gaggctcaat atatgcgtag tcaccttttt
      601 gtttaccgct gagctcaagt gcaatgtttg caagtacttc attaacattc atatttgcag
      661 aagtccctgc accaccctgg tacaaatcta ctaaaaattg atcgtgatat ttccccgcaa
      721 gtacttccgt acaggctttt tcaactgcat caagaacgta ttttggtaat gcacctgagt
      781 cataatttgc tctagcagca gcaagcttta caagaacaaa tgcatttact aattcaggat
      841 aatctttagt agttacacca ctgatttgaa aattttctaa agcgcgtaaa gtttgaacgc
      901 catagtatgc atcatttgga atctgttttt ctccgagaag atctttttcg gttctgaatc
      961 cttcctgtcc aaaaagtaca gaaggcaaaa acactaactc aaagatgatc gataagatta
     1021 atttattttt taataacata attgactcct ataaaattta taatacgaat gaactaatta
     1081 taaaagcgaa gagcgttgtt gcgattacac aaacaacacc tggaagcata aagctgtggt
     1141 tcaacaaata cttcccgatt tttgttgttc cggttctatc aaatgaaaca gcagcgagca
     1201 cagttccata agttggtaaa aagaaaaaac cgttaacagc aggataaact gcaattaaaa
     1261 cccataatgg cattcctaaa gcaacgccga caggcatcaa tgtaacgact gtagccgcct
     1321 ggctgaataa aagcatggaa agcacaaata aaccaagtgc aagcacccaa ggagcttgtt
     1381 ggataacacc tgatatacca ccaactattt cagttttatt accttcaaag aatgatgagc
     1441 ccatccatga cacaccgagt attgaaataa ctgcaactac accaccagtc ataatactgc
     1501 ccttaacagc ctcactaggt tttgctttaa agaagatcat cataattcca gcggctgcta
     1561 gcattatgat cattatagca tcacccattt ctaatttttc aattaccgat cctttccctg
     1621 ataatatttc ataacttgga cgtagttcag gaaagattcc aatgaacacg acaatcacaa
     1681 tcgatactaa aaaaattaat aatgaattac gagcgtttcc aattttttct tttgccattt
     1741 caacagcaac atttgattcc tgaattttac ctgattttaa tctctcctga tactcagtat
     1801 catccttaag atcttttcct ctccatgcca cagatagtgt tccaacaaat aatccaaata
     1861 ttgaagctgg aataattacc atcattatct gaagtaaact tacattatat gatacaagtg
     1921 cacctaataa agcaacagta gcagcactga ttggtgatga gattacacca tacattgaag
     1981 ccgtgacact gattgacaat ggtcgttcgg gacgaattcc cgccttagtt gaaacttcag
     2041 caattactgg aagtaaggca taaataacat gctgcgtacc actagcaaga attagaaagt
     2101 atgtaacgaa aggtgcaaca aatgtgatgt actgaggttt tttcctcatt acttttgtgg
     2161 caagagagat aagatagtcc aaaccacccg ctgattgcat tgctgccaat gctgtaatca
     2221 ctgcgataat cattcccaga acaattcccg gaggagaacc cggtggaaga ccgaaaagaa
     2281 aaacaaatat tactagacct aaaccaccca ttgtacctaa tccaatacca ccaagccttg
     2341 cacctatcac tatgcaggta aatagtatga gaagttcaat ccacatcatt tttgcaccta
     2401 taaaaattta atctatttta ttcaaccttt aattagatac taatatgttt cataatattc
     2461 ttgaatcttc accggatcgt tagtcttcaa taacgcaagt cttagcagaa ctcttgcctt
     2521 agatggaatt aattcgtaag aagcatcaaa accaagagca tcatcatcaa gttccacatt
     2581 ccgtccgaca aaaccggtaa taactcgtga acctcgaacc acaaccacac ccttacttct
     2641 agcatacttc aatgcttcaa cgcaggcgtc tgttaaattt ccatttccaa gaccgctggt
     2701 tactatacct tttgcaccaa aatctactgc cgctttgatc aagtcgccag gagcatcttc
     2761 ataaatagca ataacatcaa ctcttggtaa actagttagg ttatctacgt taaactcagt
     2821 ctggtaggta tgttttttat ccggaacatc tacataattt atttcaccat aatttaccgt
     2881 tcccaaggga ccaactaatg gcgagaagaa tgtttcaact gctgtagtat ttcccttgat
     2941 agctgcacga cctgaatgga tgagatcatt agcaactacc ataactcccc gatcccatga
     3001 ctctttactt cctgcaactg caactgcatt gtaaatattt aatggaccat ctgcgcttag
     3061 agctgtagaa ggacgcattg aacaagttag cactaccggt ttctttgatt taataacaag
     3121 atttaagaag taagcagttt cttccattgt atctgtgccg tgagttataa ctattccatc
     3181 aacatcatcc ttagctaata actcgttgca gcgttttgcc aatttcaacc agacctgatc
     3241 gctcatattc tggcttccga tatttgaaat ctgctcacca gtgacattag caatctcttt
     3301 cagctgggga accgcattaa ttaatatatc cactccaacc tggccggatt gataacccgc
     3361 ctgaacttca gttggtgccg ctcctgctat tgttccacct gttgcaagaa taacgatgtt
     3421 aggcagtttt tgtgcattta aggtaaatgc aactgcaaga acgataaaga aactaagtaa
     3481 ccttttcata actttactcc ttataatgat taatgactta aaatttattt ataaataggt
     3541 tcatacattt tgctcttaat aaacttcctg atgttctttg gtcttttaac gcgtgctaat
     3601 ccgcttttga aaatttcttc tgcaatttta acagctacat ttacagagac ttctcttata
     3661 ttttttattg gtggataaat tagtccattg ttaaaatctt cttctgttat ttgttcagct
     3721 actgcttcgg atgcagttag aagcatttcg tcggtaactc ttttagcttc agtagcataa
     3781 attgctaaac ccaatgcagg gaatatgaaa acattattgc cctgccccgg tgtaaatgtt
     3841 ttaccattgt aggttacagg ttcaaaagga cttccacttg caaagattgc tttgccctta
     3901 ctccaggtat aagcttgttc ggcagtacat tctgaatggg aagttggatt tgagtaaggg
     3961 aagataatcg gacgatcatt gatcgcactc atattttcaa ttacctgctg agtgaaagca
     4021 cctccaacag tgcttactcc aatgattgca gttggtttga tctttgatat actcccagca
     4081 aaatctattg atggttcact atcatgtgcg aattcttttt gatactcggc gatatctgtt
     4141 cttgatttta caagtaatcc attaacatca aacatccagc agcggttgta agattcttct
     4201 ttacttaatc catccctctg aaacttctta acaagcattt ctgcaatacc aaaagctgcc
     4261 gctccggcac caagaaaaag aaatttttgt tctttaaagg gtttattcaa caatcgactt
     4321 actgcgataa atcctgcaac tgctatggct gctgtaccct gaatatcatc attaaaggtg
     4381 cagattttgt ctcgatactt gtctaaaatc ctgattgcat ttacacctgc aaaatcttcc
     4441 cactgaatgc agacttttgg ataaactttt tcaatagctt taacgaaagc atgtatgaat
     4501 tcatcatatt ttttcccgct gatccttttc tgcttaatgc cgggatagag aggatcattt
     4561 aaaaactttt cgttatcagt tcctgcatct aaaacaattg gtagagtatg ctctggagga
     4621 acacctgcac aacttgtata caatgctaat tttcctatcg ggatacctat accacatacg
     4681 cctagatcac caagtcctaa aattctttgt ccatctgtta ctacaataaa tctaatatcc
     4741 tcaacaggcc agtttcttaa aatcgattct attttatttt tatgcttaat tgagatgaaa
     4801 agacctcttg gacgtcttgc aatatgacct aatctctgac aagcttcacc aacggtaggg
     4861 gtataaacaa gcggtaaata ttttgcaggg tcgcttataa ttgttttaaa aaataaagtc
     4921 tcattggtat caagcaatcc tgttagataa atatatttat tgataggctt attcaagttt
     4981 tctatctgtt cctggacacg cataatttgt gtttccatgg attctattac atcaggtaaa
     5041 attccattta aaccatattc tagtctttcc tgttttgtga atgccgttcc tttatttttg
     5101 cgtggattat atagcaacga atatgctttt agttcttgca tcttgtttaa tcattcattt
     5161 tattattaaa ttattttaag cataatattt ttaaaagttc atcatcgcaa caattaccgg
     5221 tccccatgct gtcaacaaaa tatttccaag tgcataaggg actgtataac ccagaaccgg
     5281 cagtttactt cctgcagcgt cctgtaaggc ttttaaccct gcggttgtag ttcctgcacc
     5341 ggattgagca cctagtagta tgactggatt aatttttaaa acataatgac caaataaaag
     5401 ccctactatg tgcggagtta ttgctacaat aagtccggca aaaagtaatc cgatacctgt
     5461 ttgacttaaa ccagaaataa aacttggacc tgcagaaagt ccaactatac caataaatgt
     5521 tgcaagccca acattatcaa aaatccataa agctgcttca ggtatgcggc caaactttgg
     5581 tgtttttgaa cgaagccaac cgaatattaa acccattaca agtgcaccgc cgcttgtagt
     5641 aagtgaaatt gaaagacctc cgattgttac agcaagtaat ccaaccaaac ctcccaataa
     5701 aattcccaaa ccaacaaata tcatatcagt ctcagctgta acgcggtcaa tgtagccaat
     5761 tagtttagct gcacgttcta catcaggtaa taatccggaa atcttaagag tatcaccacg
     5821 attgataatt gtctcagcac tgaaaggaat ttcctgacct gtccggatta atttatgaag
     5881 ctttactccc tggccgtatt ttgttcctat ttcagagaga gattttcctg caagtttttt
     5941 gtttgtaata attaaatctc tgtatgcaat tgggaaatct aaaagttcac gatccattac
     6001 ttcagggcct atgtcataga atttttgaaa caaaacattg tatctggcca tcaccgcaat
     6061 tatatctccc gattgaagaa ttgtaccggc atttggttca attatttttc cattacgacg
     6121 tattctgtga ataaaaattc ttgatgtagt ggaaatactc tgaatatcct gaacactcat
     6181 accaaaccac ttatcattgg ttattttgaa tgctcttaat ctccaatcct caaatgctga
     6241 ggcaatacct tcctcatctt ccgattttcc taaaagtttt ttctcaagct ctttgctggc
     6301 agatcgtaaa tcaattttta gaagttttgg agctaatgag gaaaggaacc atacaagaaa
     6361 agttgtacca acaagataag ttactgcata agcaacaggt atgttgttta gaaggattgt
     6421 tttttctgca tcaggaattt ggagacgttg aattgattca ctcgcggtac cgataacagt
     6481 tgattcagtg aatgcgccgg caagcattcc ggctgctgtt ccaacatcaa agctcatcag
     6541 ctttgacatg acgaaagctg taatcagaca ggttacacaa ataacaagtg taagaatcaa
     6601 ctgaggcaca gcatctttct ttagtcctct gaaaaattgc ggaccaacct tgtaaccggt
     6661 tgcaaataaa aacaaatcga agaagattac tttaacaacc ggggcgatct ctatgtgaaa
     6721 ctgaccaatt agaactccag caaacaaagt tccaagcaca gccccaactt taaaattacc
     6781 aatactgatt tttccgattg caaatcctaa tgcaagcacc agaaaaagtg ccagttcagg
     6841 atgttgctga agtatttcga taaagttgtt cattatttaa tctctttcgg aattatagaa
     6901 attgttttgt gttttttact gaatccatta cctttttctt ccaacgcttc tgttcatact
     6961 tcttgaagta tttgatctat gctgatttta agtttgagtt ctttggtttt ctctttttct
     7021 ggatatttgc tgcttgttca
//
LOCUS       DASWBY010000037         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_151831, whole genome shotgun sequence.
ACCESSION   DASWBY010000037 DASWBY010000000
VERSION     DASWBY010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151831"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VF870_00745"
     CDS             <1..194
                     /locus_tag="VF870_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9250732.1"
                     /translation="VYNILGQRVITLVDEEKPAGNYEVKFNASGLASGVYLYRLQAGD
                     PSKGSGQSFVETKKMLLLK"
     gene            378..890
                     /locus_tag="VF870_00750"
     CDS             378..890
                     /locus_tag="VF870_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250733.1"
                     /translation="MKNYKLPQIVLVLLFTVLFFYACSSDSENPVDPNNVTNSTATLT
                     LNGAGYSNRTVTLGNGIAGYSISDSLTAVQFSGKVDNDSLYFSVVFKGNQTGTINWND
                     ENGAIIYRTTSGGNYFYIGITQGTTTVSSYGAVSGKIEGTLSGKLIEASSQAELNISG
                     SFSAVRLPDM"
     gene            1174..2352
                     /locus_tag="VF870_00755"
     CDS             1174..2352
                     /locus_tag="VF870_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX9250734.1"
                     /translation="MYDYQKNNIYFAQVPGMMEELCEHELIELGAKNTKVSYRGIYFE
                     ADHSTLYRINYTSRTISRVLAPLKSFYCNNANAIMKTAEQIEWEKIFSLDQTFSITSS
                     VTKSTINNSLYASQVLKDGIADYFRSKFGKRPDVNTVNPDVRFNLFIEKDKAILSLDT
                     SGESLHKRGYRLLAGEAPMQETLAAVIIRLSKWDGEKPFLDCMCGSGTIICEALMHYC
                     RIPAQYLRKNFGFYHLPDFDESLWIKIKNEADNKIRPLPKELIYGSDKSQITLKVAME
                     NLSRLPFSDSIELAAQPFQHIKQFENGVLITNPPYGIRLGQRSDVEVLYKELGDFIKT
                     KCKGTTAFIYTGEPTLRKHIGLKTSKRIPLVNGKLEGVLLQIDSYEGSKKAKYQKKEE
                     "
BASE COUNT          850 a          371 c          441 g          783 t
ORIGIN      
        1 ctgtatacaa tattcttggt caaagagtta ttactcttgt tgatgaagaa aaacctgctg
       61 gaaattatga agtaaaattt aatgcttcag gcttagcaag cggggtttat ttgtatagat
      121 tgcaggctgg agacccttcg aaaggctcag ggcaaagttt tgttgagact aagaaaatgc
      181 tgcttctaaa ataagttcag aaaatattgt tgcgttcagt tataaaaaga gtaaaagcac
      241 gatcataata ttaacagcgg taataaaagc ctaaatctat ttaccatctg tttcccgtgc
      301 tgcttataat tattctgatt taatatttgg attaaataat aattagataa atcttttaat
      361 aaataaatgg agataaaatg aaaaattata aacttcctca aatcgttctt gttcttttat
      421 ttacggtttt gtttttttat gcatgctcta gtgattcgga gaatcctgtt gacccaaata
      481 atgttacaaa ctcaactgca acattaactc taaatggtgc tggttatagt aatagaactg
      541 taacacttgg taatggaatt gcaggatatt caatatcaga tagtttgact gctgtacagt
      601 tttccggaaa agtggataat gattcactgt atttttctgt ggtatttaaa ggcaatcaaa
      661 ccgggacaat taattggaat gatgagaatg gtgctatcat ttatcgaact acatcgggtg
      721 gaaattactt ttatatcgga attactcaag gaacaactac tgtaagcagt tacggggcag
      781 tcagtgggaa aattgaaggc acactaagcg gtaaattaat tgaagcatct tcacaagcag
      841 aattaaacat aagtggttca ttttctgcag taagacttcc ggatatgtaa taattaacca
      901 tattctggca ggaagctgtt ttacttatct atttatatta taaataaaac agcttctgac
      961 ttttttgaat taaaaatcga ctcttctaca atatcgctca ctgattctat taatctgagt
     1021 caacataatt tttattttcg cagaatttcc tatctctgac taaaattggt gcatagtatt
     1081 taaaattata tacttactgc atcgttccaa tttagacata actatatttg ttatgaaaat
     1141 taataacgaa tcaaataatt gaacggttta acgatgtacg attaccaaaa gaataatatc
     1201 tactttgcgc aggttcccgg aatgatggaa gaattgtgtg aacatgagtt gattgaactt
     1261 ggagcaaaaa acactaaagt atcttatcgt ggaatttatt tcgaagctga tcattctaca
     1321 ctttacagaa taaattatac ctcaagaaca atttcgcgtg tattggctcc gctaaaatct
     1381 ttctattgta ataatgctaa cgcaataatg aagactgctg aacaaattga atgggaaaaa
     1441 atattttcac ttgatcaaac attttcaatc acttcatcag ttacaaagag tacaattaat
     1501 aattctttgt acgcttcgca ggtattgaaa gatggaattg cagattactt ccgttcaaag
     1561 tttggtaaaa ggccggatgt taataccgta aatcctgatg tgcgttttaa tctgttcatt
     1621 gaaaaagaca aggcgatttt aagtcttgat acttccggcg aatcattgca taaaagagga
     1681 tacagacttt tagcagggga ggcaccgatg caggagactc tggctgcagt aattataaga
     1741 ttgagtaaat gggatggtga aaaaccattc ttggattgta tgtgtggttc aggcactata
     1801 atctgcgaag ctttaatgca ttactgcagg attcctgctc agtaccttag aaagaacttt
     1861 ggattttatc acttacctga ttttgacgaa tcactttgga taaaaattaa aaatgaagct
     1921 gataataaaa ttagaccttt gcctaaagaa ttgatttatg gaagtgataa atcacagatt
     1981 acacttaaag ttgcaatgga aaatttatcg cgtcttccat tttcagactc aattgaatta
     2041 gctgcacagc cattccagca tattaagcag tttgaaaatg gtgtattaat tactaatcca
     2101 ccttatggta tcagactcgg acaaagaagc gatgttgagg ttctctataa agaacttggc
     2161 gattttataa aaacaaagtg taaaggaaca acagcattca tttacaccgg tgaaccaaca
     2221 ctgagaaaac acatcggatt gaaaacatcc aaaaggattc cattagtaaa tggaaaactt
     2281 gaaggagtgc ttttacagat tgatagttat gaaggaagta aaaaagccaa gtatcaaaaa
     2341 aaagaggagt agagtattta aaattcaaaa actcaagaat taagattatt ttctacactc
     2401 gtatttattc gacaactgaa ccctctactg tgttttctta ttagg
//
LOCUS       DASWBY010000038         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_39188, whole genome shotgun sequence.
ACCESSION   DASWBY010000038 DASWBY010000000
VERSION     DASWBY010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39188"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VF870_00760"
     CDS             complement(<1..1041)
                     /locus_tag="VF870_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9250735.1"
                     /translation="MKAKLDNIVLNQVIMDSMPGLAYIFSRDGQLVAWNKRAEEIFGY
                     STEELHARKNPEFAQESDREKLRAAFIETLVKGSGSLEFTIETKSGKKIPVLTRARAC
                     EIEGEVYLIGLSIDISDLVSARAKNIELTDHITKLNEILNAENIYLKEQIRTDSHQFE
                     IVGESESLKYVLYRIQQVASTDVSVLIYGETGTGKELVARAIHKNSKRSKKPFIKINC
                     ASIPENLIESELFGHEKGAFTSAFEKRIGRFELADGGTIFLDEIGELPVSLQSKLLNV
                     LQQGEFERIGSSKTIKTNVRVIAATNKILEEEIKKGNFRSDLYYRLNVFPISITPLRE
                     RKSDIITLAEYYT"
     gene            1381..2427
                     /locus_tag="VF870_00765"
     CDS             1381..2427
                     /locus_tag="VF870_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250736.1"
                     /translation="MKNSLFALLIFSISFLTFPVFSQTGSDTTSLGLPGDNLDLFAVL
                     DLFQKSKTIEDFEKSLNEEKTGINNLDLNLDNKVDFIKVVTKQDGNDFTFILQVDVTE
                     KETQDVAVILVSKDKNDKVTMQIVGDVDLYGKDYVIEPKPPVTPNPGYVENKTDTVKV
                     SSPTTVVVVEQTPIVQYVYSPVYVPYYPPYHYAYYPPYFAAFTVVAIGVYRSNHYYYH
                     APYHGGYGNTVVIHNSYNYNNYNNYRNRSNTVNHYNSNGNYNRNNVSNNRNNVSNNKN
                     NVSNNKGNVSNNKSKGTSTTTNRPTTTNQTKTPSTTTRKQTSTNQSMNSSRSRSGGNY
                     SSGSSRGGGGGRRR"
BASE COUNT          858 a          463 c          403 g          882 t
ORIGIN      
        1 tgtataatat tctgccagtg taattatgtc tgattttctt tctcttagcg gagtaattga
       61 aatagggaac acattaagtc tataataaag atcgctgcgg aaatttcctt ttttaatttc
      121 ttcctcaaga attttatttg ttgcggcaat aactctaaca tttgttttta tagttttaga
      181 acttccaatt ctctcaaact ctccctgctg cagaacattt agaagtttag attgaagcga
      241 aactggtaat tcgccaattt catctaaaaa tattgtaccg ccatcggcca gttcaaatct
      301 gcctattctt ttttcaaaag cacttgtaaa tgcgcctttt tcatgaccaa atagttcact
      361 ctctattaaa ttctcaggaa tagacgcaca atttatttta ataaatggtt tcttacttct
      421 tttactattt ttatgaatag ctctggcaac taattctttg ccggttccgg tttcaccata
      481 aataagaact gaaacatcag tagaggcaac ctgttgaatt ctatatagaa catatttaag
      541 tgattcactt tctccaacaa tttcaaactg gtgggaatct gttctaatct gttctttcag
      601 gtaaatattt tctgcattta aaatttcgtt aagctttgtt atatgatccg tcaattcaat
      661 attttttgca cgagcagaaa ctaagtcact aatgtcaata gataacccga ttaaataaac
      721 ctcaccttca atttcacaag ctcttgctct tgtaagaacg ggtattttct ttccggattt
      781 agtttcaatg gtgaattcta atgagccgga accctttaca agagtctcaa taaatgccgc
      841 tcttaatttt tccctatcgc tttcctgtgc aaactccgga ttttttcttg catgtaactc
      901 ttcagtggaa taaccgaaaa tttcttcagc tcttttattc caggcaacta attgcccatc
      961 cctagagaaa atgtatgcaa gcccgggcat gctatccatt ataacctggt taagaactat
     1021 attatctaat tttgctttca taccttggtg ataaatcttt atatctaaaa taataaagag
     1081 tagataatta taacagcata taattaagga tgcatttcaa aaatatttgt tattaatgaa
     1141 acgaaattat tcactacgct ttaattctgt tttactctca gtttataatc agcaacttta
     1201 cagttgttaa tgaatggagg gggatccact ctcatttttt tttcagttct gttttgtatt
     1261 aatagtttta tgtttacaaa ttaaaagcac ccattaacag atatgctgcc taaaacactt
     1321 cgttgattac tgtagataat ttttgtaaac tttgatatta aattcagaag atatagagtc
     1381 atgaaaaata gtttatttgc attgttaata ttctcaatca gctttttaac ttttcctgtt
     1441 ttttctcaaa cgggtagtga cacaacatct ctaggtttgc ccggcgataa cctggatctt
     1501 tttgccgtgt tggatctttt tcagaaatcc aagacaatag aagattttga aaaatcatta
     1561 aatgaagaaa aaaccggaat caataatctt gatttaaatc ttgacaacaa agttgatttc
     1621 ataaaggttg taacaaagca agatggcaat gattttactt ttattcttca ggttgacgta
     1681 acggaaaaag agacgcagga cgttgccgtt attctggtgt cgaaggataa aaatgataaa
     1741 gttactatgc aaattgtagg ggatgtagat ctttatggta aagattatgt tatagaacct
     1801 aagcctccgg taactcctaa tcccgggtat gtcgaaaaca agactgatac tgtaaaggtt
     1861 tcttcaccaa ctactgtagt tgtagtagaa caaactccaa tagtgcagta tgtgtactca
     1921 cctgtttatg tgccctatta tcctccttat cattatgcat attatccgcc ctattttgcg
     1981 gcatttaccg tagtggctat cggcgtttat cgttcaaacc attattatta tcatgcccct
     2041 tatcatggag ggtatggcaa tacagtagtt attcataatt cgtataatta taacaattac
     2101 aataattaca gaaatagatc caataccgtg aaccattata attctaacgg taattataat
     2161 agaaataacg tatcgaataa caggaacaac gtttcaaata ataaaaataa tgtttcgaat
     2221 aacaagggca atgtttcaaa taataaatca aagggaactt caaccacaac gaatagacct
     2281 acaacgacaa atcagacaaa aacaccatcc actactacaa ggaagcagac atcaactaat
     2341 cagtcgatga attcatcccg ttcgagaagt ggaggtaatt atagtagcgg aagttccaga
     2401 ggtggtgggg gaggcagaag acgataatta aattcattat atatgaaaat gacacaataa
     2461 aaacgaaaca ccttaaaagg tttttgtcaa cctgaatcta tttcaggtac tcttttcttc
     2521 tcaaagtcga aaaattatat tacgaaataa aactatctcc ccatatgcaa aattcgtaat
     2581 aataggatta aaacagtttc tttcaa
//
LOCUS       DASWBY010000039         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_302332, whole genome shotgun sequence.
ACCESSION   DASWBY010000039 DASWBY010000000
VERSION     DASWBY010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302332"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VF870_00770"
     CDS             complement(<1..264)
                     /locus_tag="VF870_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250737.1"
                     /translation="MKTLTIFFLLIVITSLGNISAQVNARMLQYPDVSATQITFTYGG
                     DVWVVAKEGGTAYKLTSAKGTELLARFSPDGKQIAFSGNYDGNV"
     gene            complement(565..1230)
                     /locus_tag="VF870_00775"
     CDS             complement(565..1230)
                     /locus_tag="VF870_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9250738.1"
                     /translation="MGTFLGLLSAGVAIALKDPLVNMVSWAFILIRQPFKVGDRIQIG
                     EVAGDVIDIRLFQFSIMEIGNWVDAEQSTGRIIHIPNGIVFTVPQANYTAEFQYIWNE
                     IPVLVTFESDWKKAKQILTDIINKQGVLFSEGAERQLIEASKKFLIFYSKLTPIVYTL
                     VKDSGVMLTMRYMCNPRERRMTEEKIWEDVLNKFAECDDIDLAYPTQRFYNNSTGGKS
                     KKS"
     gene            complement(1621..>2090)
                     /locus_tag="VF870_00780"
     CDS             complement(1621..>2090)
                     /locus_tag="VF870_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9250739.1"
                     /translation="APIQYAAVCAFRGGISIERYLNNTRRVLKSLGLSIHKKLDKAGI
                     SAPKPEGGFYLFCNFNKHAAKLKKVGITGGSQLVQKLLEDTGVALLPGSVFGRPENEL
                     TARLAYVDFDGARALAAAETLPPYKIPDDDFLNTNCSNIIKAVDLICEWVKKN"
BASE COUNT          690 a          407 c          319 g          674 t
ORIGIN      
        1 aacgttacca tcataattac cactgaaagc aatttgtttt ccatcgggag aaaatcttgc
       61 cagcaattcc gtaccctttg cagatgtaag tttgtatgcg gttccgcctt cttttgcaac
      121 tacccaaaca tctccgccat aggtaaaggt aatctgagtt gcagacacat caggatactg
      181 cagcattctt gcattaacct gtgcagaaat attaccaagc gaggtaataa ctattagcag
      241 aaaaaagatt gttaatgttt tcataattcc tcgaaataga ttataaataa aattaaacgg
      301 agctaaattg tttttagaga ataaatgata ctttaaagct aaaatcacca gtaaaattag
      361 ttaaaccatc gcttattatt tttaacggta tggtaaacgg tggaatgttt ttataaacgg
      421 caggtaaaca agaaaaagag aagtacgctc tattatcatt aatccagcag taaatcttca
      481 gcaaataaat ttctgaatat tgttgaaaaa aaaacaaata ctgagtaaca accgtaaaat
      541 atttagttta acagaattta ggtattatga tttttttgat ttaccccccg tagaattatt
      601 ataaaatctt tgagttggat aagccaaatc tatatcatca cattctgcaa atttgttaag
      661 cacgtcttcc catattttct cttcggtcat tcttctttcc cgcgggttac acatatatcg
      721 catggttaac attacgccgc tgtcttttac taaagtataa acgataggtg taagcttact
      781 ataaaatatc agaaattttt ttgaggcttc aattaattgt ctttcagccc cttcgctaaa
      841 gagaaccccc tgtttattaa taatatctgt tagaatttgt ttcgctttct tccagtcgct
      901 ttcaaaagta acaagcacag gtatttcgtt ccaaatgtat tgaaattctg ccgtgtaatt
      961 tgcctgcggt actgtaaaaa ctatgccatt gggaatatgt attattctgc cggtgctttg
     1021 ctccgcatca acccagttac caatttccat aatagaaaac tgaaataatc tgatatcaat
     1081 tacatctcct gctacttctc caatttgtat tctgtcccca actttgaaag gttgacgaat
     1141 caatatgaat gcccatgaga ccatgttcac caatggatct tttagagcga ttgcaactcc
     1201 tgctgatagc agacctaaaa aagtccccat cgagccgaaa agttctaacc aaagattagt
     1261 cagggaaatg attataagaa aaacagccag atataaggat atcttctgcc attggtagcg
     1321 tacttttaaa tcctctatcc ggctgataat taatttttta agaagcctct gaattatcaa
     1381 aattaaaaca atgatgataa tcgacacaat gatcttgtat tgaagatccg gagagatgcc
     1441 taaattattt tctaagaatt gctgcataaa atattaattc gatagagtta tatattctta
     1501 cctgattaaa aacactataa tttatttcat ttctatagaa ccatttctta caaagtcttt
     1561 cttctgcaaa actaatttaa tatacttcct cgtatggaaa aatttttcaa caacccaaga
     1621 ttaatttttc tttacccatt cacaaatcaa atcaacagct ttaataatat tagaacaatt
     1681 tgtgttaaga aaatcatcat caggtatttt ataaggcggc aatgtttctg ccgccgctaa
     1741 agccctggca ccatcaaaat caacataggc aagacgagca gttaattcat tctctggtct
     1801 tccaaacacg ctgcccggaa gcaacgcaac accggtatct tctaaaagct tttgcacaag
     1861 ttgtgagcca cctgtaattc caactttttt aagcttggcg gcatgtttat taaagttaca
     1921 aaacaaataa aatccaccct caggttttgg tgcactaatt ccggctttat ccaatttttt
     1981 atggattgat aaacccaggg attttaaaac tctccttgta ttgttaaggt atctttcaat
     2041 actaattcca cccctaaagg cacataccgc agcatattgg atgggtgcac
//
LOCUS       DASWBY010000040         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_39220, whole genome shotgun sequence.
ACCESSION   DASWBY010000040 DASWBY010000000
VERSION     DASWBY010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39220"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VF870_00785"
     CDS             <1..920
                     /locus_tag="VF870_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX9250740.1"
                     /translation="NEVDNYFESFDKIAIQERENIFEKARKIKRPLILDGAMGSILQE
                     KKLTSNKRVWSAKANDDSQNEVITLHKDYIRAGADIITTNTFRTNPYSLISSGVTDIV
                     GSVSKAVDLAKKARGKSAVLIAGSNPPVEDCYQVERTISQKDLEWNHKVHIDALMEAG
                     CDFIMNETQSHFDEIKFISKYCGENHIPFVMNFFFPDKPKLLSGENLTDAIDYVLKFH
                     PLAIGFNCVTFEALENAVKRLRPEIIWGFYLNCGSGKLTDKNIECAISPTEYVNKAKQ
                     YLKYNPSFIGSCCGSTPEHTKKIKHLIDE"
     gene            913..1752
                     /gene="ispE"
                     /locus_tag="VF870_00790"
     CDS             913..1752
                     /gene="ispE"
                     /locus_tag="VF870_00790"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX9250741.1"
                     /translation="MNDIVVKSPAKINIGLNIINKRDDGFHNLETIFYPLNLFDEIKF
                     IKSDKFSFDSNDENLKKEHSNLIIKSKELLEKEFGIQLLVDIYLNKNIPIGAGLGGGS
                     SNAASTLKALIKLFDLKVDTKHLADLALLLGSDVPYFLNPIPSFAESRGEVLIPINFK
                     INKYLLIVNPGIHVATKWAFGLITPIKPDESLKSLIHKSKIEIDEIMNLASNDFEKIV
                     FNQFPEIKEIKEKMLDFEAIHSMMTGTGSTVWAMFDDEEAAYQSELYFKCKNYFTFIQ
                     NPI"
     gene            complement(1745..2686)
                     /locus_tag="VF870_00795"
     CDS             complement(1745..2686)
                     /locus_tag="VF870_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX9250742.1"
                     /translation="MTLHKPKKLNKQDVIGIISPASSPDEFIRVERGVKYLESLGYRV
                     KIGNNVGKNHGYLAGTDQERVDDIHSMFKDKTVKAIFTLRGGYGAFRLLDKIDYKVIK
                     NNPKIFVGYSEITSLQMAFFEKAGLMTFAGPMVAVDFYDEVSDYTNELFWATITSNKK
                     LGKLKYPENQKLPFLQKGIASGRLIGGNLAVFTALLGTPYFPNLTGKILMIEDIGELP
                     YRVDRLLNQLRLSGAFKKVKGIILGRFVDCHEHDPNKKTLTLGEVISDYIGTLKIPSI
                     YTFPHGHIKDFVTIPFGLKVNLNATKGTVEFAESAVK"
     gene            2944..>3348
                     /locus_tag="VF870_00800"
     CDS             2944..>3348
                     /locus_tag="VF870_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250743.1"
                     /translation="MKKSKILFIGGSINQTTMMHKISKELPECDAYFTPYFASGPLWL
                     MTKLKMLEFTIMGGQFKQKTLEYIKANNLKLDYEGKNDDYDLVFTSQDLIVPRSIKKF
                     KTILVQEGMTDPPDWRYHVTRFLGLGRWCASTS"
BASE COUNT         1167 a          528 c          547 g         1106 t
ORIGIN      
        1 ttaatgaagt tgataactac tttgaaagct ttgataaaat tgctatccag gaaagagaaa
       61 acattttcga aaaagctagg aagataaagc gaccattaat attagatgga gcaatgggaa
      121 gcattcttca agagaaaaag ctcacatcaa ataaaagagt atggagtgcc aaagctaatg
      181 atgattcaca aaacgaagtg ataactcttc acaaagatta tattagagct ggtgcagata
      241 taattacaac aaatactttt agaacaaatc catattctct tatatcttca ggagttacag
      301 atatagttgg aagtgtttct aaggctgttg atcttgctaa gaaggctcgt ggaaaatctg
      361 ctgttttaat tgcaggttca aacccaccgg ttgaagactg ctatcaggtt gaaagaacaa
      421 tctctcagaa ggatcttgag tggaatcata aggtacatat tgatgctttg atggaggcag
      481 ggtgcgattt tataatgaat gaaactcaaa gtcattttga tgagattaaa tttatctcaa
      541 aatactgcgg tgaaaatcat attccttttg ttatgaattt tttctttcct gataaaccaa
      601 aactgctttc cggtgaaaat cttactgatg caattgatta tgttcttaaa tttcaccctc
      661 tggctattgg ttttaactgc gttacttttg aggctttaga aaatgcagtt aaaagattaa
      721 gaccggaaat aatatgggga ttttatctga actgcggcag tggaaaactt acagataaaa
      781 atatcgagtg tgcaatttct ccaacagaat atgttaataa agcaaaacaa tatttgaaat
      841 ataatccttc atttataggt tcttgctgcg gttcaactcc tgaacataca aagaaaatta
      901 agcatttaat tgatgaatga tattgtagta aaatctcctg caaaaataaa tattggtctt
      961 aacatcatta ataaaagaga tgatggattt cataatcttg aaacaatatt ctatccacta
     1021 aatctttttg acgagataaa atttatcaag tcagataaat tttcatttga cagtaatgat
     1081 gaaaatctga aaaaagaaca ttccaacctt atcataaaat caaaagagtt acttgagaaa
     1141 gaatttggaa ttcagttgct agttgatatt tatttaaata aaaatattcc aattggcgca
     1201 ggacttggcg gtggaagttc taatgcagct tcaacactaa aagctcttat taaactcttt
     1261 gatctaaaag ttgatactaa acatttagcg gatttagcgc ttttgcttgg ctccgatgtg
     1321 ccttatttct taaaccctat tccttctttt gctgaatctc gcggagaggt tttaatccca
     1381 ataaatttta agataaataa atatcttctt atagttaatc caggaattca tgttgcaact
     1441 aagtgggcat ttggtttaat aacaccaatt aaaccggatg aaagtttgaa atctttaatt
     1501 cataaatcaa aaattgaaat tgatgagatt atgaatctgg caagcaatga ttttgaaaaa
     1561 attgttttta accaatttcc agagataaaa gagattaaag aaaaaatgct ggattttgaa
     1621 gcaatacatt caatgatgac aggtacaggt tcaactgtat gggcaatgtt tgatgatgaa
     1681 gaagcagctt atcaatcgga attatatttt aaatgtaaaa attattttac ttttatacag
     1741 aatcctattt gaccgcgctt tctgcaaatt ccaccgtgcc ttttgtagca tttaggttta
     1801 cttttaaccc gaaaggaatt gttacaaaat cttttatatg accatgagga aaagtataaa
     1861 tggaaggaat tttaagcgtc ccgatataat cagaaattac ctcgccaaga gttaaagttt
     1921 ttttatttgg atcgtgttca tggcaatcga caaatcttcc cagaattatt cccttaactt
     1981 tcttaaaagc tcctgacaat cttaactgat ttaacaatct atctacacga tacgggagtt
     2041 cgcctatatc ttcgatcatt aaaatttttc ctgtaagatt tggaaaatat ggtgtgccaa
     2101 gcaaagcagt aaaaacggct aagttaccac caattaatct tcctgaagca attccctttt
     2161 gaagaaacgg aagtttttga ttttcgggat attttaactt acctaatttt ttatttgaag
     2221 ttatagttgc ccagaaaagt tcgttcgtat aatcacttac ctcatcataa aaatccacgg
     2281 caaccattgg tcctgcaaaa gtcatcagcc cggctttttc aaaaaatgcc atttgaagag
     2341 aagtaatctc actgtatcca acaaaaattt tgggattatt cttaataact ttataatcaa
     2401 ttttatccaa taatctgaat gcgccgtagc cgccgcgcag agtaaaaatt gcttttacag
     2461 ttttatcttt aaacattgaa tgaatatcat caactctttc ctgatctgtg ccagctaaat
     2521 aaccatgatt ttttccaaca ttattgccaa ttttgacacg ataacctaaa ctttcaagat
     2581 atttaacacc tctttcaacc ctgataaact cgtctggaga agatgctgga gaaataatac
     2641 cgataacatc ctgtttattc aattttttag gtttatgaag tgtcataaga aatcctataa
     2701 ttagtctaat aatgtgtgca atttaatgaa acattgaaat tagcacaacc ttagttttaa
     2761 cgataaaatc ggctttatct tatcattatg cagttaagca ttttgatata aatcactctt
     2821 tgtttaggtt ttaattaagt ttttgcaaat ttagggcgtg ttatttttaa cacaaaaaaa
     2881 gttaaattat ttcatactat tttttccgat tttgagcatt aatcaatcta aaaaacattt
     2941 agaatgaaaa aatctaaaat acttttcatt ggtggttcaa taaatcaaac cactatgatg
     3001 cataaaatct caaaagaact ccccgaatgt gacgcttact ttactccata ttttgcaagt
     3061 ggacctttat ggttaatgac gaaacttaag atgcttgagt tcacaattat gggtggtcag
     3121 tttaaacaaa aaactctcga atatataaaa gcaaacaatc ttaagttaga ttatgagggt
     3181 aaaaatgatg attacgatct ggtattcact tctcaggatt tgattgtccc cagaagcatt
     3241 aaaaaattta agacaatttt agttcaggaa ggaatgactg atccaccgga ttggcgttat
     3301 cacgttacaa gatttttagg acttggacgc tggtgtgcaa gcacatca
//
LOCUS       DASWBY010000041         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_76841, whole genome shotgun sequence.
ACCESSION   DASWBY010000041 DASWBY010000000
VERSION     DASWBY010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76841"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1048
                     /locus_tag="VF870_00805"
     CDS             260..1048
                     /locus_tag="VF870_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9250744.1"
                     /translation="MEILAYIFAVLIGVVLGLLGGGGSILTVPILVYLLSIPAYEATA
                     YSLFIVGVSSLAGSINYMKQKLVDFKIALLFSVPSFIGVILTRKLLLPNLPDLLFTLD
                     NFEVSKNLVIMVLFSILMIMASISMIKKNNFDKDEKAESKIRLIIKFFFVGVLLGMVG
                     AGGGFLIIPSLVIFAKLDMKTAVGTSLLIIAVNTLIGFIGDLTTSMEFNWYLLISFTL
                     LGLIGIFIGIRISKFVDGKKLKPIFGWFVLVMGIYIIVKELILN"
     gene            1071..1409
                     /locus_tag="VF870_00810"
     CDS             1071..1409
                     /locus_tag="VF870_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9250745.1"
                     /translation="MHADLNITNSVINLDSESFAEKFRNDSKAVLLDVRTLQEFNAGH
                     IPNSKLIDIYLPSFPEKISELDKENNYYIYCRSGNRSYHAGVFMLQQGFKTVYNLADG
                     ILDWHEPLER"
     gene            1422..>2037
                     /locus_tag="VF870_00815"
     CDS             1422..>2037
                     /locus_tag="VF870_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9250746.1"
                     /translation="MFFKHIYETGLAQASYLVGCQATGQAIVIDPKRDIDTYLEIAEQ
                     ENLKITHIAETHIHADFLSGSRELAKSTGAEIFLSDEGGNDWQYQFPHTGLKDKDTFK
                     VGNLQFKVMHSPGHTPEHISFILTDTARSNHPSMIFSGDFVFVGDVGRPDLLEKAAGI
                     KGTQEIGAHQMFNSLKKFKALPDHLQVWPAHGAGSACGKALGAVP"
BASE COUNT          649 a          327 c          384 g          677 t
ORIGIN      
        1 gctacgtatc ctcctggccc accgcctaaa atagctaatt gatatttatt gggcataaaa
       61 agtattcctg attagttaaa ttttgtagcg aaatataaaa agtcacctaa gaaaataaaa
      121 tgtagtatat cacaattatg acttaccagt cagacttttt gtaaatgaaa ttagttgccg
      181 gcaatcaaat atgaatccct gttaaagata cagcatcata aaaggcaaaa tctacaaatt
      241 ttttaatgaa ttacactatt tggaaatatt agcatacata tttgcagtac ttattggagt
      301 ggtacttggg ttacttggtg gaggtggttc aattttaact gtgccgatct tagtttatct
      361 tttatcaatt cctgcttatg aggcgaccgc atactccctt tttattgttg gagtttcaag
      421 tttggctggc tcaataaact atatgaagca gaaactagtt gattttaaaa ttgctttgtt
      481 gttttcagtt ccatcattta taggggtgat tttaactcgg aaacttctac tccccaatct
      541 cccggattta ttattcactt tggataattt tgaagtttca aaaaacctgg taataatggt
      601 tttgttttca attttgatga taatggcatc aatctcgatg attaagaaaa ataattttga
      661 taaggacgaa aaagcagaaa gcaaaattag attgataatt aagttcttct ttgtcggcgt
      721 tttacttgga atggtaggtg ctggtggagg atttcttatt atcccttctt tagtaatttt
      781 tgctaaactg gatatgaaaa cagctgtcgg cacctcactt ttaataattg cggttaatac
      841 tttaatagga tttattggcg acttaactac ttcaatggaa tttaattggt atttattaat
      901 ttcatttaca ctgcttggct tgataggaat cttcatcggt attagaattt cgaaattcgt
      961 tgatggaaaa aaactaaaac caatttttgg atggtttgtt cttgtgatgg gtatttatat
     1021 tatagttaaa gagttaatac ttaattgagt ttaattaaat aggagaaaca atgcacgctg
     1081 acttaaacat aacaaattct gtgattaatc tggactcaga atcctttgca gaaaaattca
     1141 ggaacgattc taaggcagtt ctattagatg ttagaacctt acaggaattt aatgcaggtc
     1201 atatcccaaa ttctaaatta attgatatct atctgccttc atttcctgaa aaaatatctg
     1261 aacttgataa ggaaaataat tattatatct actgtagaag tggtaacaga agttaccatg
     1321 caggagtatt tatgcttcag caaggattta aaacagtgta taaccttgct gatggaattc
     1381 ttgactggca tgaacctttg gaaagatgat aaggaggaga tatgtttttt aaacacattt
     1441 atgaaaccgg attagcacaa gcaagttatt tagtcggatg ccaggcaaca ggtcaggcaa
     1501 ttgttatcga ccccaaaaga gatatcgata cataccttga aattgccgaa caagaaaact
     1561 tgaagataac acacattgct gaaacacata ttcatgccga ttttcttagc ggctcgcgag
     1621 aattggcaaa atccaccggt gcagaaattt ttctttctga tgaaggtggt aatgattggc
     1681 aatatcaatt ccctcacact ggattgaagg ataaagacac atttaaagtt ggcaatcttc
     1741 agtttaaagt tatgcattca cccggccata ctccggaaca catttctttt attttaactg
     1801 ataccgcaag aagcaatcat ccaagtatga ttttcagtgg tgattttgtt tttgttggtg
     1861 atgttggaag acctgatctt ctggaaaaag ccgctggaat taagggcaca caggaaatcg
     1921 gcgctcatca aatgtttaat tcattaaaaa aattcaaagc attacccgac catctgcagg
     1981 tttggcctgc gcacggtgcc ggttcagctt gcggtaaagc tcttggtgca gtgccga
//
LOCUS       DASWBY010000042         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_1579, whole genome shotgun sequence.
ACCESSION   DASWBY010000042 DASWBY010000000
VERSION     DASWBY010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..656
                     /locus_tag="VF870_00820"
     CDS             195..656
                     /locus_tag="VF870_00820"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX9250747.1"
                     /translation="MSKNFIYKYLSEEELKSISAKIGEIEKITSGELVITLKEKRNLL
                     EKHKTVRKLAEKEFVSAKIGKTKGSTGILFFIIFNVKGFSILADKGINEKVDQSVWDE
                     IAKSISDNFKQKKYYNGLVTGIEQAGKILAAHFPIQPGDINELSNEVRILD"
     gene            complement(659..>1820)
                     /locus_tag="VF870_00825"
     CDS             complement(659..>1820)
                     /locus_tag="VF870_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HEX9250748.1"
                     /translation="LKDVSCTEAWLQLTTNNIQLPATINLLKNNSVTQTFSLSTKDSL
                     LYIDSLLTNQSYKIIATMQQCNNVSNELSVTTMDTASHNFTWQTFEFGQHSSSVLYDV
                     AIIEENNIWAVGEIYMNDSLGNPDTKIYNAVRWNGLNWELKRIPILLNGTPFYPVIKS
                     IFAFNENDIWFEAGIHWDGITFKQIPFNIQWNGNVNKLWGSSSNDLYAVGKNGNIAHY
                     NGTIWSKIESGTTSILNDAWGITKEQNKTIVYCAASSFFVPGDKKILKIVDGKVDSVN
                     WGKDVRLYSAWTNNENFLYVCGEGVFVNKFGTWEQITLPPITTNSIRGNDINDVFAVG
                     SLGTIFHFNGISWQVISSYTQKENYRVDFKNNIVAICGYYNGKGFIEIGRRD"
BASE COUNT          556 a          325 c          305 g          634 t
ORIGIN      
        1 ttttttttga tttgtcatca cgagtcccgg gccatcggga cgaagtgatc tggaaatatt
       61 taagattgct tcgtcgaaga ctcctcgcaa tgacttttct tgttttttgt ttctcgtctt
      121 tcgcgtcttg ttttcagttt gactttctaa tcttaataag ggaactttgc acaagctttt
      181 gaattattaa aaaaatgtct aaaaatttta tctacaaata cctgagtgaa gaagaactaa
      241 aatcaatttc ggcaaaaatt ggtgagattg aaaaaataac ctccggtgaa cttgtgatta
      301 ctttgaagga aaaacgcaat cttcttgaaa agcacaaaac agttcgcaaa cttgctgaaa
      361 aagaatttgt ctctgctaag atcggaaaaa caaaaggatc aacaggcatt ttatttttta
      421 taatctttaa tgtaaaagga ttttctattc ttgctgataa agggattaac gaaaaagttg
      481 atcaatccgt ttgggatgag attgcaaaaa gcattagtga taattttaaa caaaaaaaat
      541 attataacgg cttagtaacc ggaatagaac aagccggaaa aattcttgcc gctcactttc
      601 ccatccagcc aggagatatt aatgaacttt ctaatgaagt taggatcttg gattagattt
      661 aatctcttct tccaatttct ataaatcctt taccgttata ataaccgcat attgctacaa
      721 tattattttt aaagtcaact ctatagtttt ctttttgagt ataactactt attacctgcc
      781 aactaattcc attaaaatga aaaatcgttc ccaaagagcc cactgcaaaa acatcattaa
      841 tatcgttacc acggattgaa tttgttgtaa ttggtggtaa agttatttgt tcccaagtac
      901 cgaacttatt tacaaaaaca ccctcaccac atacgtaaag aaagttttca ttgtttgtcc
      961 aagccgagta tagtcttaca tctttacccc aatttactga atcaactttt ccatcaacta
     1021 tttttaatat ctttttatcg cccgggacaa agaaagatga agcagcacaa tatactattg
     1081 tcttattttg ttcttttgtt attccccagg catcgtttag aattgatgtt gtcccactct
     1141 ctatcttact ccagattgtg ccgttgtagt gagcaatgtt gccattttta cctaccgcat
     1201 acaaatcatt actgctgcta ccccagagtt tgttaacatt accattccat tgaatattaa
     1261 atggaatttg tttgaacgtt attccatccc agtgaattcc tgcttcaaac cagatatcgt
     1321 tttcattgaa ggcaaaaatg gatttaataa caggataaaa aggtgttcca tttaataaaa
     1381 ttggtattct ttttagctcc cagtttagtc cattccatcg cactgcatta tatattttgg
     1441 tgtctggatt acccagcgaa tcattcatat aaatctcacc aacagcccaa atgttgtttt
     1501 cctctattat tgcaacatcg taaagtacac tgctgctatg ttgcccaaac tcaaaagttt
     1561 gccaggtaaa gttgtgggag gcggtgtcca ttgttgtaac ggacaattca ttgcttacat
     1621 tgttgcattg ttgcattgtt gcaataattt tataggattg gttggtgagt aaggaatcga
     1681 tatagagtag agagtcttta gtgctcagac tgaaggtctg agttacggaa ttatttttta
     1741 atagatttat tgttgccggt agttgtatgt tatttgttgt taattgcagc caggcttcgg
     1801 tgcagcttac atctttaagt
//
LOCUS       DASWBY010000043         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_716114, whole genome shotgun sequence.
ACCESSION   DASWBY010000043 DASWBY010000000
VERSION     DASWBY010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716114"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /gene="mtnA"
                     /locus_tag="VF870_00830"
     CDS             complement(<1..781)
                     /gene="mtnA"
                     /locus_tag="VF870_00830"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX9250749.1"
                     /translation="MNNYYSIKFLDDHLSFLDQTKLPLKEVYVDTDDYERIAVAIEKL
                     ELRGAPLIGISAAFALALSFKNVSSNHTEHFNKVYKRLASTRPTAVNLFYALDRMKKA
                     FQLITDYSDTYQTLLNEAKAIFEFEEHCSGKIAEHGFNIFTKKSNVLTHCNTGALATA
                     GFGTAFAVIKKAFDEGYVNHVYADETRPLLQGLRLTSYELEKNSIPFTVQTDSMAAVL
                     MQQNKIDLVITGADRIALNGDSANKVGTYNLAVLCNYHNIPF"
     gene            complement(933..>1662)
                     /locus_tag="VF870_00835"
     CDS             complement(933..>1662)
                     /locus_tag="VF870_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9250750.1"
                     /translation="HNVDVEGLQIVEGGKTFKWSGKYHYDLNVRDTLLTELNVFQDFN
                     PIIPDNFKKSKYICLGNIDPVLQAKVLDQMEDPQFIVCDTMNFWIEGKKDELLQLLPK
                     VDVLIINDSEARLLAHEPNLIRAARKIRELGPKILIIKKGEHGALLFTEETVFSAPAY
                     PMETIFDPTGAGDSFAGGFTGYLYKTRDLSPESLKRAVIYGSTMASFCVEKFSTKGLE
                     DLSYLQIQDRYREFMMLSRFDEQE"
BASE COUNT          516 a          313 c          269 g          564 t
ORIGIN      
        1 aaaatggtat gttatgatag ttgcaaagca cagcaagatt gtatgtgccg actttattcg
       61 cactatcccc attaagagca attctatcag caccggtaat aactaaatca attttattct
      121 gctgcattaa tactgctgcc atagaatctg tttgaactgt aaaaggaata ctattttttt
      181 caagttcgta tgaagttaaa cgtaatcctt gaagcaaggg tctcgtttca tcagcgtaaa
      241 cgtgatttac atatccttca tcaaatgctt ttttaattac tgcgaacgct gtcccaaatc
      301 ctgctgttgc aagcgcacct gtgttgcagt gcgttaacac attagatttt tttgtaaaga
      361 tgttaaaacc atgttcagca attttaccgg aacagtgctc ttcaaattca aaaattgctt
      421 ttgcttcatt taataatgtt tgataagtat cagaataatc agtgattaat tgaaatgctt
      481 ttttcattct atccagcgca taaaaaagat ttactgcagt tggtcttgtt gatgctaatc
      541 ttttataaac tttattaaaa tgttctgtat gatttgatga aacatttttg aatgaaagtg
      601 caagtgcaaa cgctgcagat attccaatta acggagcacc gcgcaattcc agtttctcaa
      661 ttgccacagc gatacgttca taatcatcag tatcaacata aacctctttc aacggaagtt
      721 tcgtttgatc cagaaatgaa agatgatcat ctaaaaattt tattgagtaa taattattca
      781 ttggtctttt ttgaattaaa attcgtactg aattatttaa acgtcatttc gaaggagtga
      841 aacgactgag aaatctgttt aaatatgtca aagatttctc atccgacaag tcgggttcga
      901 aatgacacta ctacctttgt ttattgactt ttctattcct gttcatcaaa tcttgaaagc
      961 atcataaatt ctctgtagcg atcctgtatc tgcaggtagg ataaatcctc taaaccttta
     1021 gtgctgaatt tttcaacgca gaaagaagcc attgtacttc cgtaaataac tgcacgtttt
     1081 aaactttctg gagaaagatc acgggttttg tacaaatatc ctgtaaatcc tcccgcaaaa
     1141 gaatcacctg ctcctgttgg atcaaaaatt gtttccattg gatatgctgg cgcagagaaa
     1201 acagtttctt cagtaaacaa caatgcaccg tgttcgcctt tttttatgat aagaatttta
     1261 ggaccaagtt ctcttatctt tctggcggcg cgaattaaat ttggttcgtg tgcaagcaat
     1321 cttgcttcgc tgtcatttat aatcaataca tcaacttttg gcagtaactg aagcaattca
     1381 tctttctttc cttcaatcca gaagttcatt gtatcgcaaa caataaactg cggatcttcc
     1441 atctgatcca gtacttttgc ctgcagtaca ggatcgatat tgccaagaca aatgtatttt
     1501 gattttttaa aattatcagg aatgatagga ttaaaatcct gaaaaacatt taattcggtt
     1561 aaaagtgtat cacgtacatt caaatcataa tgatatttgc cggaccactt gaaagttttt
     1621 ccgccttcaa caatctgcaa accctcaaca tctacattat gc
//
LOCUS       DASWBY010000044         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_302496, whole genome shotgun sequence.
ACCESSION   DASWBY010000044 DASWBY010000000
VERSION     DASWBY010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302496"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VF870_00840"
     CDS             <1..273
                     /locus_tag="VF870_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250751.1"
                     /translation="FSNHIFLKYPLIVKNRKLFFELAKNSRITLGDWFLSPLHPVKVD
                     LERWQLVRHNYPVADSISKKIVNLPTDIRTNKVVIKFLNQNLDLIE"
     gene            512..1672
                     /locus_tag="VF870_00845"
     CDS             512..1672
                     /locus_tag="VF870_00845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9250752.1"
                     /translation="MNKDYLLFHKPFISEEEIDEMVDTLRSGWLSMGPKTIKFEEEFN
                     KYIGSKRSVAVNSWTAAGHLTLEAFGIEKGDEVILPTMTFPATAEIVCYFGAKPVIVD
                     VDEDTLNISLTEIEKAITPKTKAIIPVHYGGQPCDLDEIQEIARAYNLKVLEDAAHSL
                     PATYKGKKIGTISDVTCFSFYATKTLSTGEGGMICTNNEEIAERCAIMRLHGINRDAW
                     KRYSESGSWYYEVVAPGYKYNFTDLQASLGLPQLKKVDVMWEWRKKIAARYFEGLKNL
                     DMITLPTVKSDRESSWHLFPIRLNLDMITKNRAQIIDELKNNNVGVGVHFMPVHQHLY
                     YSETFNLDDKNYPVASAAFPRLMSLPIYPGMTDQSIEKVIRAVIEILNKYRR"
     gene            1684..2259
                     /locus_tag="VF870_00850"
     CDS             1684..2259
                     /locus_tag="VF870_00850"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX9250753.1"
                     /translation="MGKQGSFIKRFTDIVVSLFSLFIFSPVFLISAVAIKIDSKGPVF
                     FLHERTGLNGIPFKMIKFRGMIDNALSFGPGITKLNDPRITKVGKFLRRTSIDELPQL
                     INVIRGEMSLIGPRPEIISITSNYDEYQKNVFQFLPGITGISQINGRQMLTPDERTAI
                     EIKYYSNETFLGDLKIFFKTFWVIVSNKGNI"
     gene            2384..>3091
                     /locus_tag="VF870_00855"
     CDS             2384..>3091
                     /locus_tag="VF870_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HEX9250754.1"
                     /translation="MKSVLFYAASIYFIILSISVNIFPQDYKVLQSDDEHVLLEFNFE
                     GKFNINDFIIDGIKFTKITDFNYPLQNPGDPFLPTRFYEIGIPQYSKAVLSIIDVERE
                     VIKDKFIISTPDSADQPYKTLNYNQNVYGSNALFPLDQAQINSHATYRYIKTASLSVA
                     PFQFNPVERTLVFNKRIKVRIDYKQDENFIDMVLPISDKMTEEVIYSNVINPTEALTF
                     QGKVMSVSDSPQENYWYN"
BASE COUNT         1092 a          481 c          525 g          993 t
ORIGIN      
        1 ttctcaaatc atatttttct aaaatatcct cttattgtaa aaaacagaaa actatttttt
       61 gaattggcta aaaattccag aataaccctg ggggattggt ttttatctcc tttacatcca
      121 gtgaaagtgg atttggaaag atggcagtta gttcgccata attatccagt agcggattca
      181 atttcaaaaa agatagtcaa tctaccaaca gatatccgaa ccaataaggt cgttattaag
      241 tttttaaatc agaatttgga cttaatagaa tgatattatt aaaaatgcct tcttggagtt
      301 cgtctaagat agaaagatta gaacaatagt tagtattatg aaaggtagca ttcattgata
      361 ataaaaactt tcaatacatt ttgaaagatt aaatatatta ttatctttca cctcaatttt
      421 gataattctt ggtaataacc aagatgtaat ttttacttac gtattcttat tattaaattt
      481 gaaagtcaaa accggcaaat aataattata tatgaataaa gactatttac tctttcacaa
      541 gccctttatt tctgaagaag aaattgatga aatggtggac accttgcgtt ccggctggtt
      601 gagcatggga cccaagacta tcaaatttga agaagagttt aataaatata ttggatcaaa
      661 acgatctgtc gctgtaaatt cctggactgc agccggacat ttgactttgg aagcttttgg
      721 tatagaaaaa ggggacgaag ttatcttacc aactatgact tttcctgcca ccgctgaaat
      781 tgtttgttat tttggagcta aaccagttat tgttgatgtt gatgaagata cactgaatat
      841 ttcattaaca gaaattgaaa aagcaattac acccaaaact aaagcaataa tacccgttca
      901 ctatggcggt caaccatgcg atcttgatga aattcaggaa attgcgagag cctacaactt
      961 aaaagttctt gaagatgctg ctcattcact accggcaacc tataaaggga aaaaaatagg
     1021 aactatctct gatgtaacct gctttagttt ttatgctaca aaaacactat ctacgggtga
     1081 aggtggaatg atatgcacta acaatgaaga aattgctgaa cgttgtgcaa taatgcgtct
     1141 gcatggcatt aatagagatg cctggaagcg atatagtgaa tcgggctcct ggtattatga
     1201 agttgttgca ccgggataca aatataattt tacagattta caagcatcac ttgggctgcc
     1261 tcagttaaaa aaagttgatg taatgtggga gtggcgaaaa aaaattgcag ccagatattt
     1321 tgaaggctta aaaaatttgg atatgataac tttacctact gttaaatctg atagagaatc
     1381 atcctggcat ttattcccca ttcgacttaa tcttgatatg attacaaaaa atcgtgcaca
     1441 gattattgat gaattaaaaa ataataatgt cggagtaggt gtacatttta tgcctgtgca
     1501 tcagcattta tattacagcg aaacatttaa tttggatgac aaaaattacc cggtagcttc
     1561 ggctgctttt ccgcgtctga tgtcattgcc aatttatcct ggaatgaccg atcaaagtat
     1621 tgaaaaagtt atcagagctg taatagaaat attgaataaa tatagaagat aatactcctt
     1681 aaaatgggta agcaaggaag ttttatcaaa cgttttactg atatcgtggt gagccttttt
     1741 tcactcttta tattttcccc tgtattttta atatctgccg ttgcaatcaa gattgattcg
     1801 aagggtccag tattcttttt gcatgaaagg acaggactaa atggaattcc atttaagatg
     1861 ataaaattta gaggcatgat agataatgct ttaagttttg gaccaggtat tacgaaatta
     1921 aatgatccaa gaataactaa ggttggaaaa tttctgagaa gaacaagcat cgatgagtta
     1981 ccacaactta taaatgtaat aagaggtgaa atgagcctaa ttggtccaag accagaaatt
     2041 atcagtatca cttcgaacta cgatgaatat cagaaaaatg tatttcagtt tttacctggt
     2101 atcaccggta tatcccaaat aaacggaagg caaatgctca ctcccgatga aagaacagca
     2161 atagagataa aatattattc caatgaaact ttcttgggcg atctaaaaat attctttaaa
     2221 actttttggg tgattgtaag caataaaggc aacatctaaa actaagtcta cagattataa
     2281 aacagtatta tatacctctt ttttttaccc ttgtttaact tattgttaca atttaacttg
     2341 aataacaaac atataagaaa ttcaatcacc cgggatgttt agtatgaaaa gtgtactgtt
     2401 ttatgctgct agtatatatt ttattatcct ttcgatttca gtcaatattt tccctcaaga
     2461 ttataaagtt ttacaatctg atgatgaaca tgttttgctt gaatttaatt tcgaaggaaa
     2521 attcaatata aatgatttta taatagacgg aattaagttt actaaaataa cggatttcaa
     2581 ttatccgctt caaaatcccg gcgatccatt tttaccaact agattttatg aaattggaat
     2641 tccacagtat tcaaaagcag tcttgagtat tatcgatgta gaacgagaag ttataaaaga
     2701 taaatttatt atttcaacac cggattctgc agatcagccg tataaaacct taaattataa
     2761 tcagaatgtc tatggcagca atgctttatt tcctcttgat caagctcaaa taaattctca
     2821 tgctacttat agatatataa aaactgcatc actctcagtt gctccatttc agtttaaccc
     2881 tgttgaaaga actctcgttt ttaataaaag gataaaagtt cgtatagatt ataaacaaga
     2941 tgaaaacttt attgatatgg tgttaccaat atcggataaa atgacagagg aagtgatata
     3001 ttcaaatgta ataaatccaa ccgaggcact aacctttcaa ggtaaggtaa tgtcggtatc
     3061 agactcccca caagaaaatt attggtacaa c
//
LOCUS       DASWBY010000045         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_415481, whole genome shotgun sequence.
ACCESSION   DASWBY010000045 DASWBY010000000
VERSION     DASWBY010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415481"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..1087)
                     /locus_tag="VF870_00860"
     CDS             complement(350..1087)
                     /locus_tag="VF870_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250755.1"
                     /translation="MKNILILLLISSAQIFSQIEDKSFYSFKQQAISEISLMKDLDQQ
                     FAGDEMKQKKSTGLAIVYSLLLPGMGELYADAYDSGVYFTIADGVLWGTYIGMNVYAN
                     WQKDRYISYAQTNASITTENKDGDYYATIGEYLSIDQYNDQMAFERNFNEMYNTEQYF
                     WKWNTSEERKAYREMWVSSEQTFNDVRFVVWGLLLNRVVSAINAVRLVSRYNNNLSQE
                     VGWNVSLGVQNNPDNSSSYNINFITSF"
     gene            complement(1104..2438)
                     /gene="mtaB"
                     /locus_tag="VF870_00865"
     CDS             complement(1104..2438)
                     /gene="mtaB"
                     /locus_tag="VF870_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX9250756.1"
                     /translation="MPQTVALLTLGCKLNFSETSTIGNQFLKNGFSVVNMKNKADVYV
                     FNTCTVTENAEKECRQLVRRVLRNNPNAYVIVTGCYAQLRADEISAIEGVDAVLGSNE
                     KFEIFSLLKDFDKKELSCIYVSPNDELNKFGLASSTNADSRTRAYFKIQDGCDYKCTF
                     CTIPLARGKSRSMNPELVVEEFTNLVKQGYKEIILTGVNVGDYGKSFDLNFYGLLKKL
                     IQVKGDFRIRISSIEPNLLTEEIIKLTAESEKLCNHFHIPLQSGSNTILRSMQRRYTA
                     EDYKNVILRAKELIPDAGIGVDVIVGYPGETEKEFMETYNFLLDLPISYLHVFTYSER
                     PDTKALTISESVEPSERKRRNKMLRILSDKKKNYFYNQMIGKNLDVLFESKDEDGSIK
                     GFSSNYIRLYNKFENDIDNKICDVFVEGIENGMAYGKIKEIKNSVALEISSI"
BASE COUNT          807 a          450 c          335 g          895 t
ORIGIN      
        1 attgattcca taaataatgt tgaagagttc aaaaagcttt ttgaagttga tgactaaaaa
       61 aatgctttaa ccaatacctt tttaaaaata atttcaatta tctaaatgcg ttttttatat
      121 ctaacaatct gtttgtgtta gtgaatattt attgtgtcag aattattcca atattttttt
      181 cttgcacgat gattaattat agaatttaga atttctcttt ttacttatca tctccagcgg
      241 aatctacttt tacaaactta atattaagag tagagaagta gttaagaaaa tgattataat
      301 gaaatgatga ttaaaaaaaa tatccatcaa atcgatggac atttttaatt taaaaagatg
      361 ttataaaatt aatattgtaa gaagaagaat tatcagggtt attctgaaca cccagagaaa
      421 cgttccatcc aacttcctga ctaagattat tattatacct ggatactaat cttactgcgt
      481 tgatggcgct tactacacga tttaacaaca gaccccaaac aacaaatcta acatcattaa
      541 aagtctgttc tgacgaaacc cacatctctc tataagcttt tcgttcctcg gaagtattcc
      601 atttccaaaa atattgttct gtgttgtaca tttcattaaa attacgttca aacgccattt
      661 gatcattata ctgatcaata cttaaatatt caccaatcgt agcgtaataa tcaccatcct
      721 tattctctgt agttatgctt gcattagttt gtgcataaga tatatatctg tccttttgcc
      781 agttagcata cacattcata cctatatatg ttccccacag cactccatct gcaatagtaa
      841 aataaacgcc actgtcataa gcatccgcat ataactctcc cattccaggt aacaacaaag
      901 aatagacaat tgctaagccg gtactctttt tttgtttcat ctcatcacca gcaaactgct
      961 gatctaaatc tttcatcagc gatatctcag atattgcttg ctgtttgaat gaataaaatg
     1021 atttgtcttc aatttgcgaa aatatttgtg ccgaggaaat gagaagtagt attagaatat
     1081 ttttcatttt ttttacttga atattagatt gaggatattt ctaatgcaac ggaatttttt
     1141 atctctttta ttttaccata tgccatgccg ttttcaattc cttctacaaa aacatcgcaa
     1201 attttgttgt ctatatcatt ttcaaactta ttataaagcc ttatataatt tgaggaaaaa
     1261 ccttttattg atccatcctc atctttggat tcgaacagca catcaagatt ttttccaatc
     1321 atctgattgt aaaaataatt tttcttttta tcacttaaaa ttcttagcat cttgtttcgt
     1381 cgttttcttt cagatggctc cactgattca gaaattgtta aagcttttgt atcaggtctt
     1441 tctgaataag tgaaaacatg taagtaagaa ataggcaaat caaggagaaa attataagtt
     1501 tccataaatt ctttttcagt ctctccggga tatccaacga ttacatcaac accaatacca
     1561 gcatccggaa ttaattcctt tgctctaaga ataacatttt tataatcttc cgctgtatat
     1621 cttctttgca tagatcgcaa aatggtgttg ctgccgcttt gcagagggat gtgaaaatga
     1681 ttacataatt tttccgattc ggcagtaagc tttattattt cttcagtcaa aaggtttggt
     1741 tcgattgaac taattctaat tctaaaatct cctttaactt gaatcaattt ttttaacaaa
     1801 ccataaaaat tcaaatcaaa agatttacca taatcaccaa catttacgcc ggtaagtata
     1861 atttctttat agccctgctt aacaagattt gtaaactctt ctactacaag ttcaggattc
     1921 atacttcggc ttttaccacg cgcaagtgga attgtgcaaa aagtgcattt gtaatcacag
     1981 ccatcctgaa tcttaaagta agctctggtt ctgctgtcag cattagttga acttgctaaa
     2041 ccaaatttgt taagctcatc attaggagaa acatagatac aggaaagctc ttttttatca
     2101 aaatctttta gaagtgaaaa tatctcaaac ttttcattac tgccaagcac tgcatcaaca
     2161 ccttcgattg ctgaaatttc atcagctctt aactgtgcat aacaacctgt aacaattacg
     2221 tacgcatttg gattatttct aagaactctt cttaccagct gcctgcattc tttttcggca
     2281 ttttctgtta ctgtgcaagt gttgaataca taaacatctg ctttattttt catattcaca
     2341 acagaaaatc cattctttaa aaattgattt ccaatagtag atgtttctga aaaattaagt
     2401 ttacatccta atgtaagtaa tgctactgtt tggggcattt aagtttttaa ttgatataaa
     2461 aagagcggga tgttttcccg ctcctaa
//
LOCUS       DASWBY010000046         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_152114, whole genome shotgun sequence.
ACCESSION   DASWBY010000046 DASWBY010000000
VERSION     DASWBY010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152114"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VF870_00870"
     CDS             <1..289
                     /locus_tag="VF870_00870"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX9250757.1"
                     /translation="FEADVDIYTDFSEAAKKDSLNQTIDYHKVYKVLYQLALEQKYYL
                     IEAVASKIADELLTKFAAIEKIAVRVRKNNPPLGGVVDCVEVEVIKERKDI"
     gene            293..832
                     /gene="folK"
                     /locus_tag="VF870_00875"
     CDS             293..832
                     /gene="folK"
                     /locus_tag="VF870_00875"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX9250758.1"
                     /translation="MKNDGLNIAFVGIGTNEGNKISNIKSAIGLISSLKNCKIEKISS
                     VYETLPFGDIKQDNFLNAVIKISTAFNPQELFAELKNIEQKLGRIVREKWGPREIDLD
                     ILFFNDLIFSDEIITLPHKGIIYRDFVLIPLIEIEPDLIHPVFNKKIVDFVLDLKTKN
                     IINKLSEPLFTEENYLGIK"
     gene            819..1478
                     /locus_tag="VF870_00880"
     CDS             819..1478
                     /locus_tag="VF870_00880"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX9250759.1"
                     /translation="MASNSEIKFIAVEGVIGAGKTALAKKLKSRLESKLILEQFEANP
                     FLENFYNDRKRFAFQTQMFFLINRFKQQEELNQEDLFTNFIVCDYIFDKDKIFAYLNL
                     SAEELKLYENVFPLLSRNLRKPDLVIYLQSSLDRLMFNIKKRGRAIERNLTRSYIEEL
                     SDAYNHFFFRYNSTPLLIVNSTDIDFVNNDEDFDELFRQIFREDRGVIEYFKPEARSL
                     G"
     gene            1484..1678
                     /locus_tag="VF870_00885"
     CDS             1484..1678
                     /locus_tag="VF870_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250760.1"
                     /translation="MLRFILYFIIFFILFRLLKSVISNFAKGRNSPAIKNEPQKKKSK
                     FEDVEDAKYTEIKPEDEKKN"
     gene            1685..>1942
                     /locus_tag="VF870_00890"
     CDS             1685..>1942
                     /locus_tag="VF870_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase"
                     /protein_id="HEX9250761.1"
                     /translation="MSEKSIPSNILNYIFKKLFSIKENTDKSLGDVKRILIIRQHNQL
                     GDMLAGVSLFRAVKEKYPQSHLTLLSSKDNYYGVIKNKLIDE"
BASE COUNT          723 a          255 c          321 g          643 t
ORIGIN      
        1 atttgaagca gatgtagata tttatacgga cttttcagaa gcagcaaaga aagattcact
       61 aaatcaaaca atcgattatc ataaagttta taaagtgctt taccagcttg cacttgaaca
      121 aaaatattat ctcattgaag cggttgcatc caaaattgct gatgagcttt taaccaaatt
      181 tgcagctatc gaaaaaatag ccgtgagagt tagaaaaaat aatccgcctc taggaggtgt
      241 agttgattgt gttgaagttg aagtgattaa agaaagaaaa gatatttagg aattgaaaaa
      301 cgatggccta aatatcgcct ttgttggtat tggtacaaat gaaggaaata aaatatctaa
      361 cataaaatct gcaatcggtt taataagtag tttaaagaac tgtaaaattg aaaaaatttc
      421 ttctgtctat gagacactgc catttggtga tattaaacag gataattttt taaatgctgt
      481 tattaaaata tcaactgcat ttaatccgca agaattattt gctgaattga aaaatatcga
      541 acaaaaactt ggaagaattg tacgtgaaaa atggggacca cgggaaattg atctggatat
      601 tttgtttttt aacgatttaa ttttttcaga tgaaattatt actttaccac ataaaggtat
      661 aatttataga gattttgttc taattccttt gattgaaatt gaacccgatt tgattcatcc
      721 ggtttttaat aaaaaaattg ttgattttgt tttagatctt aaaacaaaaa atattattaa
      781 taaattatct gaaccattat ttacggaaga aaattatctt ggcatcaaat agcgaaataa
      841 aatttattgc cgttgaaggt gtaattgggg ccggtaaaac tgctttggca aaaaaactta
      901 agtccaggtt ggaatcaaaa cttatacttg aacaatttga agctaatcct tttctggaaa
      961 atttttataa tgatagaaaa agatttgcat tccaaacaca aatgttcttt ttaataaaca
     1021 ggtttaaaca acaggaagaa cttaaccagg aagatctgtt tacaaatttt atcgtctgtg
     1081 attacatttt tgataaagat aagatatttg cttacctaaa tctttcagcc gaagaactta
     1141 aattatacga gaatgttttt ccgctgcttt caagaaattt aagaaaacct gatttggtta
     1201 tttatcttca atccagcctt gacagattaa tgttcaatat taaaaaacgg ggcagagcaa
     1261 ttgaaagaaa tcttacgcga agttatatag aagaattaag cgacgcttac aaccatttct
     1321 tttttagata taattctaca ccacttctta ttgttaattc aacagatata gattttgtaa
     1381 ataacgatga agattttgat gagttgttcc gtcagatatt cagggaagat cgtggagtaa
     1441 tagagtactt taaaccagaa gcaagaagtt taggttaata atcatgctgc gtttcatttt
     1501 atactttata atatttttta ttctctttcg tttattaaaa tcagtgattt ctaactttgc
     1561 taaaggcaga aacagccctg ctattaaaaa tgagcctcaa aagaaaaaat caaagtttga
     1621 agatgttgag gatgctaagt acactgagat caaacctgaa gatgaaaaga agaactgatg
     1681 taaaatgtca gagaaatcaa ttccatctaa catattaaat tatattttca aaaaattatt
     1741 ttctattaaa gaaaataccg ataaaagcct tggtgatgta aaacgaattt tgataatccg
     1801 tcagcataac cagcttggag atatgcttgc aggggtttca ttgtttaggg ctgttaaaga
     1861 aaaatatcct caatctcatc ttacactttt gtccagtaaa gataattatt acggtgttat
     1921 aaaaaataaa ttaattgatg ag
//
LOCUS       DASWBY010000047         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_1627, whole genome shotgun sequence.
ACCESSION   DASWBY010000047 DASWBY010000000
VERSION     DASWBY010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VF870_00895"
     CDS             <1..1447
                     /locus_tag="VF870_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX9250762.1"
                     /translation="EGKDACMLLLREILITKEKQHLLDNTILLIIPVLNVDGHERISP
                     FNRPNQNGPKKMGWRTNALNLNLNRDYLKADSSEIKSFLNLFNEWLPDFMIDNHTTNG
                     ADYQYHVTYGIETHQNIDRGLLIFIEKKYLPNLLQKVEDDGFIIGPYMEFKTGTIESG
                     IVDLPAPPRLSHGYCATQNRICLLVETHSLKPFANRVFSTKSMMEHSISFVNKNHKEL
                     ISLNKQADKETVKNYLSTKKKFPLVLKGNGKFDKYLFKGFEWYDEYSELTGSTVRKYT
                     SEPIEIEIPIFNKANSEKKISVPAAYLIPQQFSNIIDVLKTHHINFNILKSTKNVKVE
                     KYRFENVQFAPRPYEGRQLPTFKCYAFTETVAVEAGTLIVYTNQRQLRVIVNLLEPEA
                     PDSFVKWGFFNAFFERKEYAEAYVMEPYAKQMIKSDPDLRSEFYKKLNEDEPFRNNEG
                     ERLDFFYKCSPFYDKGENVYPIMRLINKTDI"
     gene            1581..2777
                     /locus_tag="VF870_00900"
     CDS             1581..2777
                     /locus_tag="VF870_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9250763.1"
                     /translation="MLTILCISSYEKGFDFMREAKTQGCRVILLTSKSLENADWPRES
                     LDEIFYIPDKNKDWNMQDVIYGVSYLARTEQIDRIVALDDFDVERAASLREHLRIAGM
                     GDTTARHFRDKLAMRMVAKEKGIPVPEFCHILNHKKINEFADTVPYPYMIKPRLLAGS
                     YGLKKVNNKQEMWDRINYLADEQSFFLMERFVPGNIYHVDTIINEREVIFGLASKYGT
                     PPFEVAHQGRVFTSQTLDSKSDEAKEVLELNKKVLKALGLLRGVSHSEFIRAEDGTLY
                     FLETSARVGGANLSSLVEAATGINLWREWAKIEIFNGEKPYQLPEVKNNHAAILTSLS
                     KQEWPDLDAYNDPEVYWRLRKEYHAGLIIKSSNKERISQLLDEYTKRFYNDFFATAKM
                     GERPSD"
     gene            2977..>3507
                     /locus_tag="VF870_00905"
     CDS             2977..>3507
                     /locus_tag="VF870_00905"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEX9250764.1"
                     /translation="MEILIKENDSLQSETSNKTEVLNQLEGKVKEWMEDHVDKRKLWF
                     SSDFLPADEKMNDDQESNLKILRERVLGVKDEVRVAVALNLLTEEGLPHFHRLIAQYL
                     GDRSFWSKWNNMWTAEEDRHGAVIRDYVRDARLFSFREVELMQFHYIESGFNPDWDKD
                     PYKVFVYTTLQERATQF"
BASE COUNT         1246 a          550 c          667 g         1044 t
ORIGIN      
        1 agagggtaaa gatgcttgta tgctgctgct cagggaaatt ctcatcacaa aagaaaagca
       61 gcatctttta gataacacaa ttttattaat aattcctgtc ctaaatgttg atggacacga
      121 aagaataagt ccatttaaca gaccgaacca aaatggaccg aagaaaatgg gctggcgtac
      181 caacgccttg aacttgaatt tgaatcgtga ttatttaaaa gcagattcat ctgaaataaa
      241 atcattccta aatttattca atgaatggct accggatttt atgattgata atcacaccac
      301 aaacggcgct gattatcagt accatgttac ttatggaatt gaaactcatc aaaatataga
      361 tagaggctta ttaattttta ttgaaaaaaa atatcttcca aaccttttac aaaaggttga
      421 agatgatggg tttattattg gtccttatat ggaatttaaa actggcacaa ttgaaagtgg
      481 aattgttgat ttgccggcac cgccaagatt atctcatgga tactgcgcaa cacaaaacag
      541 aatttgcttg ctcgtcgaaa ctcatagctt aaaacctttt gccaatcgtg tgttctcaac
      601 caaatctatg atggaacatt cgataagttt tgtcaataag aatcataaag aattaatatc
      661 gctaaataaa caagctgata aggaaacagt taaaaattat ttatcaacta aaaagaaatt
      721 tcctttggtg ctaaaaggaa atggaaagtt tgataaatat ttatttaaag gatttgaatg
      781 gtatgatgaa tatagcgaac taaccggctc aacggttaga aaatacacca gtgagccaat
      841 agaaattgaa attccgattt tcaacaaagc aaactcggaa aagaaaatta gtgtgcctgc
      901 cgcttattta attccgcagc agttttcgaa tattattgat gtgcttaaaa ctcatcatat
      961 aaattttaat attctaaaat caacaaaaaa tgttaaagta gaaaagtaca gatttgaaaa
     1021 tgttcagttt gctccaaggc cttacgaagg aagacaactt ccaacattta agtgttatgc
     1081 atttactgaa acagttgccg ttgaagccgg aactttaatc gtttatacaa atcaaagaca
     1141 gctgcgtgta attgtaaatc tacttgaacc tgaagcacct gattcgtttg ttaaatgggg
     1201 attctttaat gcgttctttg aacgaaagga atatgcagaa gcctatgtaa tggaaccata
     1261 tgcaaagcaa atgatcaaaa gcgatccaga tttacgatct gaattttaca aaaaattaaa
     1321 cgaagacgag ccttttagaa ataacgaagg ggaaagattg gatttctttt ataaatgctc
     1381 cccgttttat gataaaggag aaaatgttta tccaataatg aggctaataa acaaaaccga
     1441 tatttgattt tgatgaaatt aagtttaaaa tttccagtgc aataaactaa gttaaactta
     1501 gtgttctaag tttcttggtg gtaaattctt cttcaccact cagacactga gagcatacag
     1561 aaccactaag gagaaaagtt atgctaacaa ttctgtgtat ttccagttat gaaaaaggtt
     1621 ttgattttat gcgcgaagcc aaaactcaag gctgtcgtgt aatattatta acctctaaaa
     1681 gtttagaaaa tgctgattgg ccaagagaaa gtcttgatga gattttttac atcccggata
     1741 aaaacaaaga ttggaatatg caggatgtaa tttacggtgt tagctatctt gcacgtactg
     1801 agcagattga tagaattgta gcacttgacg attttgatgt tgagcgtgcc gcttctttaa
     1861 gagagcattt aagaatagcc ggaatgggtg atactactgc aaggcacttc cgtgataagc
     1921 tagcaatgag gatggttgca aaagaaaaag gaattcctgt ccctgaattt tgtcatatat
     1981 taaatcataa aaagataaat gaatttgcag atactgtccc ctatccttat atgataaaac
     2041 cacgtttact tgccggatca tacggattaa aaaaagtaaa caacaagcag gaaatgtggg
     2101 atagaattaa ttatctcgca gacgagcagt cattcttttt aatggaacgc tttgttcccg
     2161 gcaacatcta tcatgttgat acgataataa atgaaagaga agttattttt ggattagcaa
     2221 gcaaatacgg aacaccacct tttgaggtcg cacatcaggg aagagtgttt acaagccaaa
     2281 cactggatag taaatctgat gaagcaaaag aagttttaga acttaataaa aaagtactaa
     2341 aggctttagg attattacga ggtgtatccc actctgaatt tattcgtgct gaagatggga
     2401 cactttattt ccttgaaaca tctgcacgag tcggcggagc taatctttcc tctcttgtag
     2461 aagctgctac tggaattaat ctttggagag agtgggcaaa gattgagata tttaatggtg
     2521 agaaacccta tcaacttcca gaagttaaaa acaaccacgc tgctattcta acttctctat
     2581 caaaacaaga gtggccggat cttgatgctt ataatgatcc tgaagtttat tggaggttga
     2641 ggaaagaata tcatgcggga ttgatcataa agtcatcaaa caaagagaga attagccaat
     2701 tacttgatga atacacaaaa aggttttata atgatttctt tgcaactgca aagatgggcg
     2761 aaagaccaag cgattagtat tcaatttatg attttaattc tgtcacactt agcttgtcga
     2821 agtgtgacag atttttccat attacagttt ttaacgattt gtaaatttaa tgtaaaacct
     2881 catttggggg tgttcaatac ctttctagtt attcaaatca tttttattat caatcagtaa
     2941 aactaaacga ttaaacaaaa taacatttac ctttttatgg aaattttaat aaaagaaaat
     3001 gattcactac aatctgaaac atcaaataaa actgaagttc taaatcaact tgaaggcaaa
     3061 gtaaaagaat ggatggaaga tcacgttgac aaaagaaaat tatggttctc aagcgatttc
     3121 cttcctgccg atgagaaaat gaacgacgac caggaaagta atcttaaaat acttcgcgag
     3181 cgtgtacttg gagtgaaaga tgaagttcgt gttgcagtcg cattaaatct tttaacagaa
     3241 gaagggttgc cgcattttca cagattaatc gcacagtacc taggcgatag aagtttttgg
     3301 tctaaatgga ataatatgtg gacggcagag gaagacaggc acggtgctgt tatcagagat
     3361 tatgttcgtg atgcacggtt gttcagtttt cgtgaagttg aattgatgca gtttcattac
     3421 atagagagcg gatttaatcc tgattgggat aaagatcctt acaaagtttt tgtttacaca
     3481 acacttcaag aaagagcgac tcaattc
//
LOCUS       DASWBY010000048         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_415686, whole genome shotgun sequence.
ACCESSION   DASWBY010000048 DASWBY010000000
VERSION     DASWBY010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415686"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VF870_00910"
     CDS             <1..363
                     /locus_tag="VF870_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250765.1"
                     /translation="VECIDDINSAYYNTIVSRDKIEKVDWQSSEKMFFADIWYEQGII
                     VEQNTNPVVDGAGSCIFLHNWSTPNETTAGCTEMEPARLKEIIYWLDSSANPVLVQLT
                     KQLFIEYQQSWELPSPQN"
     gene            546..>3720
                     /locus_tag="VF870_00915"
     CDS             546..>3720
                     /locus_tag="VF870_00915"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HEX9250766.1"
                     /translation="MSSKIILLFIIAVTFLSNSQNKSQNILSSDKKIEIKNNDFENLS
                     GSSSVLNWQFSNNSTTSVDGSISESGLNSILIFHNDWNQSEVISDPVELTIGHLYKLS
                     AWIKTEDAVTDPIDRYPTSVAACISMFSLPFTNNSQSVGATTKWTKIELSFIATQKSD
                     QIRLQFGNNGKAKGKVWFDNIELFKVADISDYIPAETVRWFNNGFRYDDQGWIFLHIE
                     GAPYDRGYQYGNLVAEELVEYINKIGIQQNKDIPSKGWDNLKFVADAMMLRKYETEYL
                     TEMKGIADGVNNTGVKLFNRKLDLLDIVTLNSAIDIDYSKDAMGVTPNQLSGHSFLKS
                     EDELNISNRLHKCSSFLANKSSTTDGRIVFGQIFMWGGYTGPHWNIICDVVPTEGNRL
                     VYQTFPGGIHSGSDFYINSSGIMIGETTVGQTPFNVDGSPQSNRIRKAAQYSNSIDDV
                     VKILTTDNNGMYTNDWLIGDTKTDEIAVLLLGTKKHKLWRSSNNEFYGDTKDFYWCNN
                     NNKDPEVRKEYITNPNNAPYDLIFSPWNRDIAFNDFYKEQKGKIDAIAGVNLWASSPI
                     NRPHACDGKVTTSDMAENLVFLAHYGKVTLREMFVGENGRIPDLPGAFPRLSLGYSVI
                     SPIFITDKIKEQRNQKIINVNKKEFKINYNEVIDKYSYDEKKLWMNTVYPASESENWF
                     VSGSAAYWSILKGIPKSKNDVLKYFKDQFAELNARYLYTVNKEGSISPINAKRIYTGY
                     NNYQIPRIKGTYILHQLRLLLGNDVFSKLMLTTHEKYREKEMKTEDFISLANQISGKD
                     LTSFINQWLKRDDLPDPEIKSVALSSEGKNKLQIEIDQLNKQYNFLCTVELKSDSEKT
                     YKLLEVTDLKQSFSFDIAGDNSGIEFNSFYDIPIKHENYYTWSNIFDDWKTAKIVYGT
                     KRQIEANHTLALRFSTTLADRFTEDLIPIIKDCELDEEQLRNNDLIVMGNVEDNSIMK
                     LFCDQLNIKVGKNFFEWNDKTYGRSDEGLFLTLPNPYNNKKIIYLFITNSALELHHMT
                     KIVNRRSQWALFRWDKIIEQGYYNPNNQ"
BASE COUNT         1334 a          542 c          671 g         1173 t
ORIGIN      
        1 gttgaatgta tagacgatat taactccgct tattataata cgattgtaag tagagataaa
       61 attgaaaagg ttgactggca atcatcagaa aaaatgtttt ttgctgatat ttggtatgag
      121 cagggtatta ttgtggaaca aaacacaaac cctgttgtcg atggagccgg ttcttgtatt
      181 ttccttcata attggtccac acccaacgaa accactgctg gctgcacgga aatggaaccg
      241 gcaagactaa aagaaataat ttattggctt gattcttcag ctaaccctgt tctcgtgcaa
      301 ttaacaaaac aactttttat agagtatcaa caatcttggg aactaccttc ccctcaaaat
      361 taataaggtt tagtagcccc atataaagaa ggtagtttgt ttctctttaa ttttttcttt
      421 caatcctatt gaatatctga gttgcttttt ttgatgtttg tatccagttc ttagtctttt
      481 acagaaagtt aaattatttt ttagttaaga ttataaaatt atccatcaat atgaggattt
      541 ccggtatgtc atctaaaatc attttgcttt ttattattgc agtaacattt ttatctaact
      601 cgcaaaacaa atctcagaat atattgtcat cggacaaaaa aattgaaata aagaataatg
      661 attttgaaaa tctttcaggt tcctcgtctg ttctaaactg gcaattctct aataattcta
      721 caacctctgt ggatggaagc atttccgaat cgggattaaa cagcatttta atatttcata
      781 atgattggaa tcaaagtgag gtaatttctg atccggttga gttaacgatt gggcatcttt
      841 ataaacttag tgcgtggatc aaaaccgagg atgctgtaac agatccgata gatcgttatc
      901 ctacctctgt agctgcatgc atatcaatgt tctcacttcc attcactaat aattctcaat
      961 ctgttggagc aactacaaaa tggacaaaga ttgagctttc ttttatagca acacaaaaat
     1021 ctgatcagat aagattacag tttggaaata atggcaaagc taaaggaaaa gtttggtttg
     1081 ataacatcga gctctttaaa gttgcagata tatctgatta tatacctgca gaaacggtca
     1141 gatggttcaa taacggattt agatatgatg atcagggatg gatattttta cacattgaag
     1201 gcgcgcctta tgatagaggt tatcaatatg gaaatcttgt agcagaagaa ttagttgaat
     1261 acataaataa aataggtatt cagcaaaaca aagatattcc ctctaagggt tgggataatc
     1321 tcaaatttgt tgccgacgca atgatgctga gaaaatatga aacagaatat cttaccgaaa
     1381 tgaaaggtat tgctgatggt gtaaacaata ctggagtaaa gctttttaac cgtaaacttg
     1441 atttactgga tattgtgaca ttaaactcag caattgatat tgattattca aaagatgcga
     1501 tgggtgttac accaaatcaa ttatcaggac atagttttct taaaagtgaa gatgaattga
     1561 acatttcaaa ccgtcttcac aagtgctcaa gttttcttgc taataaatca tcaacaactg
     1621 atggtagaat tgtattcggt cagattttta tgtggggtgg atatactggt cctcattgga
     1681 atattatttg cgatgttgta cctacggaag gtaatcgatt agtttatcaa acatttccag
     1741 gaggaattca ttcaggttca gatttttata taaattcttc cggtataatg ataggcgaaa
     1801 cgactgttgg tcaaacacca tttaatgttg atggttcgcc tcaatccaac agaatcagaa
     1861 aagcagcaca atattcaaat agtatagatg atgttgttaa gattctcact actgataata
     1921 acggaatgta tacaaacgat tggctgattg gtgatactaa gaccgatgaa attgctgtac
     1981 tgttgctggg aacaaaaaaa cataaactct ggaggagcag caataatgag ttttatggtg
     2041 atacaaaaga tttttattgg tgcaataaca ataataaaga tccagaggta cgtaaggaat
     2101 acataacaaa tccaaacaat gccccgtatg atttaatatt ttcaccctgg aacagagata
     2161 ttgcgttcaa tgatttttac aaagagcaaa aaggaaaaat tgatgcaata gcgggagtaa
     2221 atctttgggc aagttcgccg attaacagac cgcacgcttg tgatggaaaa gttacaactt
     2281 ccgacatggc tgaaaatctt gtattccttg ctcattatgg taaagtgact ctaagagaaa
     2341 tgttcgttgg tgagaatgga agaattcctg atctgcccgg agcatttcca cggttatcat
     2401 taggatattc agtgataagt cctattttca ttactgataa gattaaagag caaaggaatc
     2461 aaaaaattat aaatgttaac aagaaagaat ttaaaatcaa ttacaatgaa gttattgaca
     2521 aatattctta tgatgaaaaa aaattatgga tgaatactgt ttatccggca tctgaatcag
     2581 aaaattggtt tgtcagtggt tctgcagctt attggtcaat acttaaagga attccaaaat
     2641 caaaaaatga tgttttaaaa tactttaaag atcaatttgc agaacttaat gcaagatatc
     2701 tttacacagt aaataaggaa ggatcaatta gtccgatcaa tgcaaaaaga atttataccg
     2761 gatataataa ttatcagata cccagaataa aaggcactta tattttacat cagttaagat
     2821 tgcttttagg aaatgatgtt ttttcaaaat tgatgctgac cacacacgaa aaatatcgtg
     2881 aaaaggaaat gaagactgag gattttattt ctttagcaaa tcaaataagc ggtaaggatt
     2941 taacatcttt tatcaatcag tggttaaaac gagatgatct gcccgatccc gaaattaaat
     3001 cagttgctct tagctctgaa ggtaaaaata aactgcaaat agaaattgac caactaaata
     3061 agcaatacaa ctttttgtgc actgtggaac ttaaatcaga tagcgaaaaa acatacaagt
     3121 tgttggaggt tacagattta aagcaatctt ttagctttga tattgccggt gataattctg
     3181 gaattgagtt taactcattt tatgatattc caataaaaca tgaaaattat tacacctggt
     3241 caaacatttt tgatgattgg aaaacggcaa agatagttta tggaaccaaa agacaaattg
     3301 aagctaatca taccttggca ttaagattta gcacaacgtt agctgataga tttactgaag
     3361 atttaattcc aataattaaa gattgtgagt tagatgaaga acagttaaga aataatgatt
     3421 taattgtgat gggtaatgtt gaagataaca gcattatgaa attattttgt gatcagctta
     3481 atattaaagt tggaaagaat tttttcgaat ggaatgataa aacatatggc cgatcagacg
     3541 aaggattatt tttaacacta ccaaatccat acaataataa aaaaattatt tatttattta
     3601 taacaaacag tgcactggaa ttacatcaca tgactaaaat agttaataga aggtctcagt
     3661 gggccttatt tagatgggat aaaattattg agcaaggata ttataatcca aataatcaat
//
LOCUS       DASWBY010000049         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_265134, whole genome shotgun sequence.
ACCESSION   DASWBY010000049 DASWBY010000000
VERSION     DASWBY010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265134"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..726
                     /locus_tag="VF870_00920"
     CDS             34..726
                     /locus_tag="VF870_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX9250767.1"
                     /translation="MKKVHFTIFIILSIIFMEQTLLAQNSDWANFGKYKEDNLKIGLP
                     LPDENRVVFMGNSITQGWGEINPEFFKDKPYVNRGISGQTTPQMLVRFRADVVNLKPK
                     VVVILAGTNDIAGNTGPSTLEMIEDNISSMVEIAAANNIKVILCSVLPAYDYPWKPGL
                     EPAQKIVALNKWIKEYADKNEIIYVDYFTPMADERSGLKKEYSEDGVHPNLAGYRIME
                     PLVEKAIVDALR"
     gene            902..>1646
                     /gene="secA"
                     /locus_tag="VF870_00925"
     CDS             902..>1646
                     /gene="secA"
                     /locus_tag="VF870_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561135.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX9250768.1"
                     /translation="MLNLIKKIFGDKHEKDLKVLWPIVNEINEHYETIKNLTNDELVN
                     KTKEFKEKIQAHTEETRKQIHELQTRLQSDEDFDRNAAYDELDELEEKLNDEYEEILD
                     KLLPEAYAVVKSTCQRLVGTSWTVAGNKLTWDMVPYDVQLIGGIVLHQGKIAEMGTGE
                     GKTLVATLPMYLNSLTGRGVHLVTVNDYLAKRDSEWMGEIFRFHGLTVGVILNTMDSA
                     QRQQQYACDITYGTNNEFGFDYLRDNMSVD"
BASE COUNT          574 a          259 c          320 g          493 t
ORIGIN      
        1 ttggtttatt aaacaagtat tggattgata attatgaaaa aagttcattt tacaatcttc
       61 ataattttat ctataatatt tatggaacaa actctacttg cacaaaacag cgactgggca
      121 aactttggca aatacaaaga agataactta aaaatcggat tgcctttacc agatgaaaat
      181 cgggtagtgt ttatgggtaa ttcaattaca cagggatggg gagagattaa tcctgaattc
      241 tttaaagata aaccttacgt taacagagga atcagtggtc aaacaactcc tcaaatgctg
      301 gtaagattta gagcagacgt tgtaaacctt aaacctaagg ttgtggtaat tttagcgggc
      361 actaatgata tcgctggaaa caccggacct tcaactttag aaatgattga ggataatatt
      421 tcttcaatgg ttgaaattgc agcagctaac aatattaaag ttatactttg ctcagtgctg
      481 cctgcatatg attatccttg gaaaccgggg cttgaaccgg ctcaaaaaat tgtcgctctt
      541 aataaatgga taaaagaata cgctgataaa aacgaaatta tttacgttga ttattttact
      601 ccaatggcag atgagcgcag tggtttgaag aaagaatatt ctgaagatgg tgttcatcct
      661 aatttagctg ggtatagaat catggaacct ttagttgaaa aagctatagt agatgctctc
      721 agataggaac tactcaaaat ctgtctgcta taaatgatgt aatctaggct aaatactcga
      781 tgttttaccg cacttactgc aaatatcgat caaattcttt atccaattat tcttaaattg
      841 catctcaaat catctatctt taatttttca atgtaattac taacggagaa atttacgcgc
      901 aatgttaaat ttgattaaaa aaatatttgg cgataaacac gaaaaagatc ttaaagttct
      961 ttggccgatt gttaatgaaa ttaatgagca ttatgaaacc atcaaaaatc ttacaaatga
     1021 tgagttagtt aacaaaacaa aagagtttaa agaaaaaatt caggctcata ccgaagaaac
     1081 aagaaaacaa atacatgaac ttcaaacccg cttacaatcg gatgaagatt ttgatagaaa
     1141 tgcagcatac gatgaacttg atgaactgga agaaaaactt aacgatgagt acgaagaaat
     1201 actggataaa cttttacctg aggcttatgc tgttgtaaaa tccacttgcc agagattagt
     1261 tggcacaagc tggaccgttg cgggtaataa gcttacctgg gatatggttc catacgatgt
     1321 acagcttatc ggcggaatcg tacttcatca aggcaagatt gctgaaatgg gaactggtga
     1381 aggaaaaact cttgttgcaa ctttgccaat gtatcttaat tcattaactg gtcgcggtgt
     1441 gcatcttgtt actgttaatg attacttagc taaacgcgat agtgaatgga tgggagagat
     1501 attcagattt cacggtttga ctgttggtgt aatattaaat acaatggatt cggcacagcg
     1561 ccagcagcaa tacgcctgcg atattacgta cggaacaaac aatgaatttg gtttcgatta
     1621 tcttcgtgat aatatgtcgg ttgata
//
LOCUS       DASWBY010000050         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_152424, whole genome shotgun sequence.
ACCESSION   DASWBY010000050 DASWBY010000000
VERSION     DASWBY010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152424"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..>1847)
                     /locus_tag="VF870_00930"
     CDS             complement(13..>1847)
                     /locus_tag="VF870_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX9250769.1"
                     /translation="GYGPEGDILEGTKKVKASDEVDMKLLLTGAALCSNARLLPPNEE
                     SARYTVLGDPTEACLGVVAKKSGINIPKQAELTPRLRELPFESRRKRMTTIHELEQPI
                     DGTRRIAYVKGAPKEVMKLSESIRVNGEVQLMTEERRKEIMEANDGYARDGLRVLAVA
                     YRLLHKDDDIPIAMSTYTPEIIEQDLVFVGLIVMADPPRPEVSDAVEECRRAGIRIVM
                     ITGDYGLTAESIAKRIGIVKGASPRVVSGLELEEISDDQLKEYLKDEIIFARVAPEQK
                     LRVVSNLQEMGEIVAVTGDGVNDAPALKKADIGVAMGIAGTDVAKEAADMILTDDNFA
                     SIVHAIEEGRTVYSNIRKFLLYILNSNVPEAIPSAMFLFSRGAIPLPLTVMQILTIDL
                     GTDMLPALGLGTENPEKGIMDQPPRNQKESLLNKKLVVKAFLWYGLLGSITSAISYFF
                     INVHNGWPKVSLASGTDPIYIKATAMTLAAIVFSQIGAVYNCRTEKQSLFKVGLFSNK
                     QVNFGIIFEIFLIAALVYLPSLQSIFHTAALDLSDCLLLCVWPPLILLIEEARKAWIR
                     RKDVKNHHKKYRGKGHESYCSRLRKNGNRIITEPYEKGSSSIRD"
BASE COUNT          552 a          400 c          296 g          599 t
ORIGIN      
        1 tcaggattag agtcaatcac ggatacttga tgaccctttt tcataaggtt cagtgataat
       61 cctattccca ttcttcctaa gcctactaca ataactttca tgccctttcc ccctatattt
      121 tttatgatga tttttaacat cttttctacg aatccatgct tttcttgctt cttcaatcag
      181 caagattaac ggtggccaaa cacataataa caaacaatct gataaatcga gtgccgctgt
      241 atggaaaata gattgtaatg atggtaaata aactaaggcg gcaatcaaga aaatttcaaa
      301 gataattcca aagttcactt gtttattact aaataatcca actttaaaga gggattgttt
      361 ttcagtacga caattatata ctgcaccaat ttggctgaat acaattgctg caagtgtcat
      421 agcagttgct ttgatgtaaa taggatcagt tccgcttgca agtgaaactt tcggccatcc
      481 attgtgtaca ttgataaaaa agtaagagat tgctgaagtg atagagccta acaacccata
      541 ccataagaat gctttaacaa ctaatttttt gtttaacaat gattcctttt ggttacgtgg
      601 cggttgatcc ataattcctt tttcagggtt ttcagttccg agtcccaatg ctggtagcat
      661 atcagttcct aagtcaatgg ttaggatttg cataactgtt aaaggcaatg gtatagcccc
      721 acgagaaaat aggaacatgg cagacggaat tgcttcaggc acatttgaat ttaaaatgta
      781 taacaggaat ttacgaatat tgctataaac tgtacgtcct tcttcaatag catgaacaat
      841 agaagcaaaa ttatcatctg tcaaaatcat atccgccgct tcttttgcta catctgtacc
      901 agcaataccc atagcaactc caatatctgc tttcttaagg gccggtgcat catttacacc
      961 gtcgcctgtt acagcaacaa tttcccccat ttcttgtaga ttggatacaa ctcgtaattt
     1021 ctgttctggt gctacacggg caaaaataat ttcgtctttt aaatattcct ttaactggtc
     1081 atctgatatt tcttctaact ccaatcctga tactacgcga ggactagcac ctttcacaat
     1141 tccgatacgt tttgcaatac tttcggctgt taaaccataa tctccggtaa tcatgacaat
     1201 acgaataccc gcacggcggc attcttcaac agcatctgat acttctggcc gtggaggatc
     1261 agccataaca atcaaaccaa caaaaactaa gtcttgctca ataatttcag gagtataagt
     1321 actcatagct attggaatgt catcatcttt atgaagcaaa cgatatgcga cagctaagac
     1381 acgtaaccca tcacgcgcat atccatcatt agcttccatg atttctttac gtctttcttc
     1441 tgtcatcaat tgaacttctc catttacacg gatagattca cttaatttca tcacctcttt
     1501 aggagctcct tttacatatg caatgcgtct agttccatca ataggttgtt ctaattcatg
     1561 aattgttgtc attcttttac ggcgtgattc aaatggcaat tcccttaaac gaggtgttaa
     1621 ttctgcttgt tttggtatat taatacctga tttcttagct acaactccta aacaagcttc
     1681 tgttggatca cctaatacag tgtaacgagc agattcctca tttggtggta atagacgagc
     1741 attactgcat aaagcagccc ctgttaacaa gagtttcata tctacctcat cactcgcctt
     1801 aacttttttg gtgccttcta aaatatctcc ttcaggacca tatccaa
//
LOCUS       DASWBY010000051        17186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_39695, whole genome shotgun sequence.
ACCESSION   DASWBY010000051 DASWBY010000000
VERSION     DASWBY010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 17186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17186
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39695"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1872
                     /gene="fusA"
                     /locus_tag="VF870_00935"
     CDS             <1..1872
                     /gene="fusA"
                     /locus_tag="VF870_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9250770.1"
                     /translation="QINIIDTPGHVDFTVEVERSLRILDGAVALFCAVGGVEPQSETV
                     WRQADKYGVPRLAFINKIDRTGADFYHALEMMKERLGANAIPINLPIGEGDMFVGVID
                     LIEFNARMFQEEAFGSTFDDMPIPQDLLEIATKYRTLMLEAVSDVDDTLLEKYLDGKE
                     ITADEIKRVLRQATIDLKIIPVLCGSAFKNKGVQKLLDGVVDFLPSPIDSQEIEAHHI
                     GLNDSVKRKISEDEHFTALAFKIMNDPFVGKLTFFRVYAGSLKSGSYIFNSISGKKER
                     ISRLLQMHANHREEISEVKAGDIAAAVGLKFTRTGDTLCSEDDPVVMERIVFPEPVIQ
                     IAIEPKTKADQDKLSDALSKLSDEDPTFKVKVDEETGQTLISGMGELHLEILVDRMRR
                     EFKVEANIGKPQVAYRETITQSVQAEGKFVKQSGGRGKFGHVWVELTPNEVGKGFEFI
                     NGIVGGVVPKEYIPAVSHGIQDAMKNGVIAGFPMVDIKAKLYDGSYHDVDSDEISFRV
                     AGSMAFQAGAKKANPVLLEPIMSVEVVTPEEYLGDVMGDLNSRRGKIEGFSARKDAQV
                     IKALVPLSEMFGYATVLRSMSQGRAIYSMQFDHYSSVPKSIAEEISEKTLGKKSAAN"
     gene            1918..3123
                     /gene="tuf"
                     /locus_tag="VF870_00940"
     CDS             1918..3123
                     /gene="tuf"
                     /locus_tag="VF870_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX9250771.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHVDHGKTTLTAAITMALARKGLSAI
                     RTFDSIDNAPEERERGITIATAHVEYSTANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPKMVVFLNKVDMVDDPELIELVEEELRDLLTE
                     YEFPGTEIPIIKGSALQALEAGASNADISDPRYNCIWELMDAVDSYIPVPERSTDKPF
                     LMPVEDVFSITGRGTVATGRVERGQVKIQEEVELIGLGLHKKTVVTGIEMFRKELDAA
                     MAGDNAGILLRGVDKNEIERGMVLAKTGSITPHTNFEGSVYVLSKDEGGRHTPFFNGY
                     RPQFYFRTTDVTGVATLPEGTEMVMPGDNVNLSIELIAPIAMEEKLRFAIREGGRTVG
                     SGVVTKIIK"
     gene            3209..3517
                     /gene="rpsJ"
                     /locus_tag="VF870_00945"
     CDS             3209..3517
                     /gene="rpsJ"
                     /locus_tag="VF870_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX9250772.1"
                     /translation="MPGQKIRIKLKSYDHILIDKSTEKIIKTVRSTGAVVSGPIPLPT
                     KKTIYTVLRSPHVDKKSREQFETRAHKRIIDIHNSNNKTVDSLSKLDIPAGVDIEIKL
                     "
     gene            3535..4158
                     /gene="rplC"
                     /locus_tag="VF870_00950"
     CDS             3535..4158
                     /gene="rplC"
                     /locus_tag="VF870_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX9250773.1"
                     /translation="MSGIIGKKLGMSNIFGKDGEIIPVTVIQAGPCMVVNIRTKEKNG
                     YEAFQLSFGERKEKHTSKPLAGQYKSSGVKPARLLKEFRNFDAGDYKVGDQIGADFFV
                     EGEKIKVRGKNKGKGFQGVMRRHNFGGVGGTTHGQSDRLRAPGSIGASSYPSRVFKGQ
                     RMAGRMGYENVTIQGLKVVKVIPEQNIIFVKGAVPGAVNTILELIKK"
     gene            4172..4819
                     /gene="rplD"
                     /locus_tag="VF870_00955"
     CDS             4172..4819
                     /gene="rplD"
                     /locus_tag="VF870_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX9250774.1"
                     /translation="MTLDVLKIDGSKSGDKIDLSDSIFAIEPNDHAIYLSVKAYLANQ
                     RQGTSKSKERGEVRGGGKKPWKQKGRGGARAGTSRSPLWVGGGTIFGPRPRDYRQDLP
                     KKVRRLARKSAFSYKAKDAQLLVVEDFNIEQPKTKEFSKILNALKVNGKKILLLTEAN
                     NPNVYKSGRNIPKVKVLEASKASTYDLLNNQILILQKSAVKEIEKTFAAGAEVVS"
     gene            4822..5118
                     /gene="rplW"
                     /locus_tag="VF870_00960"
     CDS             4822..5118
                     /gene="rplW"
                     /locus_tag="VF870_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX9250775.1"
                     /translation="MNNILVRPLITEKMTNISADQAGKYGFLVSPKANKIEIKKAIEK
                     KFDVHVVEVRTINHPGKVKSQFRKSGRFEGRTARYKKAIITLKEGEKIELFEQV"
     gene            5140..5964
                     /gene="rplB"
                     /locus_tag="VF870_00965"
     CDS             5140..5964
                     /gene="rplB"
                     /locus_tag="VF870_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX9250776.1"
                     /translation="MGIKKLKPTTPGSRFRSNSTFEEITKTTPEKSLTGVIKKSGGRN
                     NLGHITTRFIGGGHKRRYRIIDFKRNKPGIPAKVFSIEYDPNRTCRIALLHYADGEKR
                     YILAPEGLKVGSKIVSGPGSDIIIGNALPLKEMPLGSFVHNVEMKPGKGGQLGRSAGA
                     SLQVMAKEGDFAQLKMPSGEVRLIRLECMATYGVVGNSEHENLSQGKAGRNRWRGIRP
                     SVRGVAMNPVDHPMGGGEGKTSGGGHPVSPWGQKAKGLKTRKRKKESNKHIIKRRK"
     gene            5982..6266
                     /gene="rpsS"
                     /locus_tag="VF870_00970"
     CDS             5982..6266
                     /gene="rpsS"
                     /locus_tag="VF870_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX9250777.1"
                     /translation="MPRSVKKGPFLDYKLAEKIKKLNDTNQKKIIKTWSRSSTIFPDF
                     VGHTIAVHNGNKMIPVYVTENMVGHKLGEFAPTRIFRGHPGTKAEKASKG"
     gene            6286..6630
                     /gene="rplV"
                     /locus_tag="VF870_00975"
     CDS             6286..6630
                     /gene="rplV"
                     /locus_tag="VF870_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX9250778.1"
                     /translation="MEARAINRYIGSSPRKMRLVIDLIRGMRVDKALEVLHFSSKHSS
                     KDAEKTLRSAVANLLNSDEATRVEPQDLYVKEVFVDQGPTIKRISPAPMGRAYRIRKR
                     SCHLTVIVASKV"
     gene            6647..7288
                     /gene="rpsC"
                     /locus_tag="VF870_00980"
     CDS             6647..7288
                     /gene="rpsC"
                     /locus_tag="VF870_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX9250779.1"
                     /translation="MGQKTHPVGFRVGVIRGWDSNWYENKSYAFKLKEDDKLRTYIRS
                     RLKKAGISRIVIDRTSKNIILTIHTSRPGVVIGKSGKEITQLEEELKRVTSKDVKVQI
                     SEIKRPELDAYLVAENIANQLAGRVSFRRAMKTSITAAMRMGAEGIRIMCAGRLGGAE
                     MARTEQYKEGRIPLHTIRADIDYANGRAETIYGSIGIKVWICRGEILGKRVTE"
     gene            7312..7728
                     /gene="rplP"
                     /locus_tag="VF870_00985"
     CDS             7312..7728
                     /gene="rplP"
                     /locus_tag="VF870_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX9250780.1"
                     /translation="MLMPKRVKYRKSQRGRRAGKAYRGSSVAFGDFGLKAMEPAWITS
                     RQIEACRVALTRKMKRDGKVWIRIFPDKPVSKKPLETRMGKGKGAPEFWVAVIKPGRV
                     MFEVAGISKELASEALKLCSHKLPIKTKVVSRPDIE"
     gene            7746..7979
                     /gene="rpmC"
                     /locus_tag="VF870_00990"
     CDS             7746..7979
                     /gene="rpmC"
                     /locus_tag="VF870_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX9250781.1"
                     /translation="MKLYEIKEMSSEELVKRIEEEEKNLIDLRFSHQLKQLNNTSKLA
                     LAKKDIARMKTILTERQKADKAAQTDKKEGAKA"
     gene            7983..8240
                     /gene="rpsQ"
                     /locus_tag="VF870_00995"
     CDS             7983..8240
                     /gene="rpsQ"
                     /locus_tag="VF870_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX9250782.1"
                     /translation="MEQRTLRKTRIGVVVSNKMDKTITVAIERKVPHPIYKKYFKKTT
                     KLMAHDEKKECGLGDKVKIMETRPLSKNKRWRLVEIVEKAK"
     gene            8255..8623
                     /gene="rplN"
                     /locus_tag="VF870_01000"
     CDS             8255..8623
                     /gene="rplN"
                     /locus_tag="VF870_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX9250783.1"
                     /translation="MIQEETNLIVADNSGAKRVRCIRVLGGSDRKYATVGDTIVVSVK
                     TAIPNGTVKKGEVSRAVIVRTKKEVRRKDGSYIRFDENAAVLINAQNEPKGTRIFGPV
                     ARELRERQFMKIVSLAPEVI"
     gene            8637..8963
                     /gene="rplX"
                     /locus_tag="VF870_01005"
     CDS             8637..8963
                     /gene="rplX"
                     /locus_tag="VF870_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX9250784.1"
                     /translation="MKIRKNDSVMVIAGNDKGKTGKVLKVFPKTSRIIIEGINLRKRH
                     TKPTQKSPQGGIQEKEAAIHSSNVMILDPKSNEPTKIGARIILDEKSGKKKIARVSKI
                     SGEMIP"
     gene            8994..9641
                     /gene="rplE"
                     /locus_tag="VF870_01010"
     CDS             8994..9641
                     /gene="rplE"
                     /locus_tag="VF870_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX9250785.1"
                     /translation="MAEKKTKKSDKEPQKKGSTKEAQSAQEKEIKITPRLGEIYKNDI
                     VPALVKTFSYKSVMQVPKLDKIVVNMGVGAAVADSKIMDEAVKELETITGQKVSVRKS
                     KKAISNFKLRENMNIGAMVTLRKERMYEFLDRLINVALPRVRDFKGLSDKSFDGRGNY
                     TLGVKEQIIFPEINVDKITRVMGMDVTLVTTAKTDNEAFELLKAFGIPFRKKEVK"
     gene            9660..9929
                     /gene="rpsN"
                     /locus_tag="VF870_01015"
     CDS             9660..9929
                     /gene="rpsN"
                     /locus_tag="VF870_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX9250786.1"
                     /translation="MARLSIIARDAKRRKLNEKYKKLREELKEKGDYDALQKLPRNSS
                     PVRLKNRCMFTGRGRSYYRKFGVSRLVLREMALRGEIPGLKKSSW"
     gene            9942..10337
                     /gene="rpsH"
                     /locus_tag="VF870_01020"
     CDS             9942..10337
                     /gene="rpsH"
                     /locus_tag="VF870_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX9250787.1"
                     /translation="MSVTDPISDFLLRIRNASKAKKLRVEIPASKMKVGLAEILKKES
                     FIHDYSIVEDNKQNVIKIQLKYRDGSSAITGMKRISKPGLRVYKDASQLPRVLNGLGT
                     AVISTSKGLLTDKEARSQSAGGEVICYIW"
     gene            10360..10899
                     /gene="rplF"
                     /locus_tag="VF870_01025"
     CDS             10360..10899
                     /gene="rplF"
                     /locus_tag="VF870_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX9250788.1"
                     /translation="MSRIGKKIIEVPKGVTVTLGGNIVKVKGPKGELQRACHPNMKIE
                     MKDAQIEVKRPDDLKQNRALHGLTRALIQNMILGVTEEYKKDLDIVGVGYKVELKGTN
                     LLLNIGYSHPIYFIPPTGIKIEATTPTHIIVSGIDKQLVGQVAAKLRSIRKPEPYKGK
                     GIKYSNEQIVRKAGKTAGK"
     gene            10922..11287
                     /gene="rplR"
                     /locus_tag="VF870_01030"
     CDS             10922..11287
                     /gene="rplR"
                     /locus_tag="VF870_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX9250789.1"
                     /translation="MIKRNTNSRLRSKTRIRKKISGTTEVPRLTIYRSLNQVYAQIID
                     DSTGSTLVSASSLSKELADEIKNSKGKVSKSKLVGSLVAKKALEQKISKVVFDRNGYR
                     YHGRVQAIAEGAREAGLKF"
     gene            11292..11365
                     /locus_tag="VF870_01035"
     tRNA            11292..11365
                     /locus_tag="VF870_01035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11324..11326,aa:Gln,seq:ttg)
     gene            11414..11926
                     /gene="rpsE"
                     /locus_tag="VF870_01040"
     CDS             11414..11926
                     /gene="rpsE"
                     /locus_tag="VF870_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX9250790.1"
                     /translation="MKAVKSTDTEGLKEKVVHINRVAKVVKGGRRFSFNAIVVVGNGN
                     GVVGVGLGKANEVTDAISKGIDDAKKNVVKVSLIKGTVAHEIIGKFGAGKVLLKPASP
                     GTGLIAGGGVRAVLEAAGITDILTKSLGSSNPHNQVKATLDGLLKQIDARKMASKRGI
                     TIKDLFIRSN"
     gene            11936..12121
                     /gene="rpmD"
                     /locus_tag="VF870_01045"
     CDS             11936..12121
                     /gene="rpmD"
                     /locus_tag="VF870_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX9250791.1"
                     /translation="MAKIKITQTRSIIDRPKTQKATIEALGLGRPNWVKIHNDTPQIR
                     GMVKKVIHLVKVEELKD"
     gene            12134..12592
                     /gene="rplO"
                     /locus_tag="VF870_01050"
     CDS             12134..12592
                     /gene="rplO"
                     /locus_tag="VF870_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX9250792.1"
                     /translation="MDILSNLKYAAGSKKNRKRVGRGEGSGHGNQSTRGMNGQMSRSG
                     AKHKAWFEGGQMPLQRRVPKFGFTNIFKVEYQVVNLISLQKLVDAKVIKDQTLNAEEL
                     KKLGLVSSSKKPIKVLGKGDVKSKLNLAVSDFSKSAQQKIEAAGGTITKV"
     gene            12603..13937
                     /gene="secY"
                     /locus_tag="VF870_01055"
     CDS             12603..13937
                     /gene="secY"
                     /locus_tag="VF870_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX9250793.1"
                     /translation="MSKFTDTFRNIFKIHELRQRILYTLALLFIVRLGSHLTIPGVDA
                     TLLAQSMKNSSSDNLFGLYDLFVGGAFSNAAIFALGIMPYISASIILQLMGAVVPYFQ
                     KLQQEGEEGRKKITQLTRYGTVLISAMQAWGVTVKLLNTQFQGLSIVPQPVQGIGWVL
                     STIIILTSGTMFMMWMGEQITDKGIGNGISLIIFIGIIARFPHSIFDEIRLISAGQRS
                     IIIEIIIFAFMFFIIAGVVLVTQGTRKIPVQYAKRVVGRKVYGGVTQYIPLRVNTAGV
                     MPIIFAQSIMFIPTTVLSFFPDNEFLQLVGGWFQYRSPIYSTVYALMIIFFTYFYTAI
                     AFNPKDVADNMKKQGGFIPGIRPGKQTSEFIDNILTKITLPGSVFLAIVAILPAFVSA
                     WGVSGSFAQFFGGTSLLIIVGVALDTLQQIESHLLMRHYDGFMKSGKIRGRR"
     gene            13942..14703
                     /gene="map"
                     /locus_tag="VF870_01060"
     CDS             13942..14703
                     /gene="map"
                     /locus_tag="VF870_01060"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX9250794.1"
                     /translation="MILIKSKKEIDYIRESCRIVAETLQLLKSNVRLGITTLELDKIA
                     EDYIRSNNAIPAFKGYSQGGAPGFPGSICSSVDDEVVHGIPGSRVLKDGEIISLDIGV
                     IKDGYFGDAALTVAVGNISDEKKKLMEVTDRSLQLGIEQAVAGNKVHDISNAVQVYVE
                     ANGFSIVRDLCGHGVGKYLHEDPSIPNFGRKGTGPKLKNGMTLAIEPMVNAGKYDVVT
                     AKDGWTILTADGSTSAHFEHTILINNNSPEILTVC"
     gene            14704..14922
                     /gene="infA"
                     /locus_tag="VF870_01065"
     CDS             14704..14922
                     /gene="infA"
                     /locus_tag="VF870_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX9250795.1"
                     /translation="MAKQGPIKIDGIITETLPNATFRVKLDNGHEIHAHISGKMRMHF
                     IKILVGDKVSIELSPYDLTKGRITYRYK"
     gene            14934..15047
                     /gene="rpmJ"
                     /locus_tag="VF870_01070"
     CDS             14934..15047
                     /gene="rpmJ"
                     /locus_tag="VF870_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX9250796.1"
                     /translation="MKVRASVKKICDKCKVIKRKGVVRVICKNPKHKQRQG"
     gene            15066..15449
                     /gene="rpsM"
                     /locus_tag="VF870_01075"
     CDS             15066..15449
                     /gene="rpsM"
                     /locus_tag="VF870_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX9250797.1"
                     /translation="MARIAGVDLPKNKKVLYGLQYIYGIGEHTASTILEKAKVSPVKK
                     VSDLTEEEVAEIRSVITAEYRVEGALRSEVQQNIKRLMDIGSYRGVRHRKSLPARGQR
                     TRTNSRTRKGKRRTVAGKKKAPSKK"
     gene            15476..15862
                     /gene="rpsK"
                     /locus_tag="VF870_01080"
     CDS             15476..15862
                     /gene="rpsK"
                     /locus_tag="VF870_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX9250798.1"
                     /translation="MAKTVKKVKKKTHVDSNGVAHIKATFNNVLVTITDIYGNTVSWS
                     SAGKNGFKGSRKNTPFAAQVSAEAAAKEAYDLGMRKIEVFVKGPGSGREAAIRALHTA
                     GLTISSIKDVTPIPHNGCRPPKKRRV"
     gene            15882..16508
                     /gene="rpsD"
                     /locus_tag="VF870_01085"
     CDS             15882..16508
                     /gene="rpsD"
                     /locus_tag="VF870_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX9250799.1"
                     /translation="MARYTDSVCKLCRRERQKLFLKGQKCYTEKCPIEERNYPPGQHG
                     LSRRAKVSEYGVQLREKQKIKRSYGLLETQFRNYFEKATSQKGRTGENLIKLLERRLD
                     TVVYRLGFASSRKQARQLITHRHIIVNNVPVDIPAYLLKAGDVIQVKEKSKKLDAIHN
                     SLKRVKDNTYGWLTVDKATLSGTFVQIPERADVPLNANEQLVVELYSK"
     gene            16541..>17186
                     /locus_tag="VF870_01090"
     CDS             16541..>17186
                     /locus_tag="VF870_01090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX9250800.1"
                     /translation="MSVSSLKMPEAVVLDEANYTNSFGRFTLQPLERGYGVTLGNSLR
                     RVLLSSLQGAAITSVKFSGVLHEFTTIEGVVEDVSEIILNLKQVRMKFLSKKPNKIDI
                     SLSGQGEWTAADIQKASNEVEILNPELHIATLNKSAKLDIEIRVGKGVGYVPAQENIV
                     PDQTIGVIPIDSIFTPIKLVKYDVENVRIGDKNDFEKLTLEITTDGSITPDDALT"
BASE COUNT         6199 a         2441 c         3624 g         4922 t
ORIGIN      
        1 caaataaata ttattgacac acccggacac gttgatttta cggttgaagt tgaaagatca
       61 ctcagaatat tagatggtgc tgttgcactt ttttgtgctg tgggtggagt tgaaccacaa
      121 tctgaaacag tttggcgtca ggcggataaa tatggtgtac ctagactagc ttttatcaat
      181 aagattgata gaactggtgc agatttttat catgctcttg aaatgatgaa ggaaagattg
      241 ggtgcaaatg caatcccgat taatcttcct attggtgaag gagatatgtt tgtcggcgtt
      301 attgatttga ttgaatttaa tgccaggatg tttcaggaag aggcttttgg atctactttt
      361 gatgatatgc caattccaca agatttgtta gagattgcta caaaatatag aactcttatg
      421 ttagaggctg tatcggatgt tgatgataca cttttagaaa aatatcttga tggaaaagaa
      481 attactgctg atgaaataaa aagagtacta agacaggcaa caattgatct aaaaattatt
      541 cctgttttat gcggatcggc atttaagaac aaaggtgttc aaaaattatt ggatggagtt
      601 gtagattttt taccatcacc aattgattcg caagaaattg aagctcacca cattgggctt
      661 aatgattcgg taaaaagaaa aattagtgaa gatgaacatt ttactgcatt agcatttaag
      721 atcatgaatg atccatttgt tggaaaactt acatttttca gagtctatgc aggttcgtta
      781 aaatccggat cctatatttt taactctatt agtggtaaga aggaaagaat aagccgactc
      841 ttacaaatgc atgctaatca tagagaagaa atttcagaag ttaaagcagg agatatagca
      901 gcagcagttg ggctaaagtt tacaagaact ggtgatactc tttgttctga agatgatcct
      961 gttgttatgg agagaatagt atttcctgaa ccagttattc aaattgctat cgaaccaaaa
     1021 acgaaagctg atcaagataa attatctgat gcattgtcaa aattatctga tgaagatcca
     1081 acatttaaag ttaaagttga tgaagaaacc ggacagactt taattagcgg aatgggagaa
     1141 cttcatttgg aaattcttgt agatagaatg agacgtgaat ttaaagttga agcaaatatt
     1201 ggtaagcctc aggtagcata cagggaaaca ataactcaaa gcgtacaagc tgagggtaaa
     1261 tttgttaagc agtctggtgg ccgaggtaag tttgggcatg tttgggttga gttaacacct
     1321 aatgaagttg gtaaaggttt tgaatttatt aatggtattg taggcggagt tgttcctaaa
     1381 gaatatatac ctgctgtttc gcatggtatt caggatgcga tgaagaatgg agttatcgct
     1441 ggatttccaa tggttgatat caaagcaaaa ttatatgatg gttcatatca cgatgttgac
     1501 tcagatgaaa tatcatttag agtagctggc tcaatggcgt ttcaagcagg cgctaaaaaa
     1561 gctaatcctg ttttactaga acctattatg tctgtagaag ttgttacacc tgaagaatat
     1621 ttaggtgatg ttatgggtga tttaaactcc agaagaggaa agatagaagg attttctgcc
     1681 agaaaagatg cacaggtaat taaagcactt gtacctttgt cagaaatgtt tggatatgct
     1741 actgtcttaa gatcgatgag tcagggcaga gccatatact caatgcagtt tgatcattat
     1801 tcatctgttc caaaatctat tgcagaagaa atttctgaaa aaacattagg aaaaaaatca
     1861 gcagcaaact aataactgga ttaaaaaact taaaaaaaat tattaaagga gaattcgatg
     1921 gctaaagaaa aatttgatag gagtaaacct cacgttaacg ttggaacaat tggtcacgta
     1981 gatcatggca aaaccacatt aaccgctgcc atcacaatgg ctctcgccag aaaaggatta
     2041 tcagcaataa gaactttcga tagtattgat aatgcacctg aagagagaga aagaggtatt
     2101 acgatcgcaa cagctcacgt tgaatattca acagctaacc gtcactatgc acacgtagac
     2161 tgtcctggtc acgcagatta tgttaaaaac atgatcacag gtgctgccca gatggacgga
     2221 gctatattag ttgtagcggc tacagatggt ccgatgcctc aaacaagaga gcacattctc
     2281 cttgctcgac aggtaggtgt accaaagatg gttgtatttt taaataaagt tgatatggtt
     2341 gatgatccgg aattaattga attagttgaa gaagaattaa gagatttatt aactgaatat
     2401 gaatttccag gtactgaaat tcctatcata aaaggttctg cacttcaggc acttgaagca
     2461 ggtgcatcaa atgcagatat cagcgatcca agatacaatt gtatttggga actaatggat
     2521 gctgttgata gctatattcc ggttcctgaa agaagtacag ataaaccatt cttaatgcct
     2581 gttgaagatg tattttcaat tacaggtcgt ggtacagttg ctacaggtag agttgaaaga
     2641 ggccaggtaa aaattcagga agaagttgaa ttgattggtt taggattaca caagaaaaca
     2701 gttgtaaccg gaatcgaaat gtttagaaaa gaattagatg ctgctatggc aggtgataat
     2761 gctggtatct tgttaagagg tgttgataaa aatgaaattg aaagaggtat ggttctggca
     2821 aaaaccggtt ctataactcc tcatacaaat tttgaaggat cagtttatgt tctttcaaaa
     2881 gatgaaggtg gaagacacac tccattcttc aatggttaca gaccacagtt ttatttcaga
     2941 actacagatg ttaccggtgt tgctacattg ccagaaggta ccgaaatggt tatgcctggt
     3001 gataatgtta acttatcaat tgagttaatt gctcctatcg ctatggaaga gaaattaaga
     3061 tttgctattc gtgaaggcgg tagaacagta ggttcaggtg ttgttacaaa gattattaaa
     3121 taattagttt aaaattattt agtgttgctc ttgaaaaggg agttaaaact cccttttctt
     3181 atctaattta atataaggag ttgattaagt gcccggtcaa aaaataagaa ttaaattgaa
     3241 atcgtacgat catattttga ttgataagtc taccgagaaa attattaaaa cggttagaag
     3301 tactggtgcg gttgtatccg gaccaattcc tcttccaact aaaaaaacta tctatacagt
     3361 tttacgttca cctcacgtag ataagaaatc cagagagcaa tttgaaacaa gggcgcacaa
     3421 aagaataatt gatatccata actcaaacaa caaaaccgta gactctttaa gtaaactgga
     3481 tattcctgcg ggcgttgata ttgagattaa gctataatga atattggaga taagatgtct
     3541 ggcataattg gtaaaaaatt gggcatgtct aatatcttcg gtaaagacgg agaaattata
     3601 cctgttactg ttatacaagc aggaccatgt atggttgtaa atattagaac taaagaaaaa
     3661 aatggctatg aagcttttca attatctttt ggtgaaagaa aagaaaaaca cacttcaaaa
     3721 ccattggctg gtcaatacaa aagcagcgga gtcaaacctg caagactttt aaaagaattt
     3781 agaaattttg atgctggtga ttataaagta ggcgatcaga ttggtgccga cttttttgtt
     3841 gaaggtgaaa aaattaaagt ccgaggtaag aataagggta agggatttca gggtgttatg
     3901 agacgtcata attttggtgg tgttggtgga acaactcacg gccaaagtga tagacttaga
     3961 gctcccggtt ctataggtgc aagttcgtat ccttcaagag tttttaaagg ccagagaatg
     4021 gcaggcagaa tgggttatga aaatgtaacc attcagggat taaaagtagt taaagttatt
     4081 ccagaacaaa atataatttt tgttaaaggc gcagttcctg gtgccgtgaa tacaatttta
     4141 gaattgatta agaagtaatt cggttaacaa aatgacgctt gatgttttaa aaatagatgg
     4201 atcaaaatcg ggtgataaaa tagatttatc cgatagcatt tttgcaattg aacctaatga
     4261 ccacgctatc tacttatctg ttaaggctta cttagcaaac caaagacaag gtacatccaa
     4321 gtctaaggaa agaggagaag ttagaggcgg tggaaaaaaa ccctggaagc aaaaaggtcg
     4381 tggtggtgct cgtgccggta cgtcccgttc tccattatgg gttggcggcg gaaccatttt
     4441 tggaccacgc ccaagagatt accgacagga tcttcctaag aaggttagaa gattagctcg
     4501 taaatctgct tttagttata aagcaaagga tgcgcaactt ttagttgttg aagattttaa
     4561 tatcgaacag ccaaagacaa aagaattttc taaaattctt aacgctttaa aggttaacgg
     4621 caaaaaaata cttttactta ctgaagcaaa caatccaaac gtttataaat caggaagaaa
     4681 tattcctaaa gtaaaagtgt tggaagcatc aaaagcatca acatatgatt tgttaaataa
     4741 tcagatattg attttacaaa agagtgctgt taaagaaatt gaaaaaacat ttgctgcagg
     4801 tgcagaggtg gtaagttaat tatgaataat atcttagtaa gacctttaat aacagagaaa
     4861 atgactaaca tcagtgcaga tcaagctggt aagtatggct ttttagttag tccaaaagca
     4921 aataaaattg aaattaaaaa agcaattgaa aaaaagtttg atgttcatgt tgtggaagtt
     4981 agaactataa atcatcctgg caaagttaaa tcacagttta gaaaaagcgg acgatttgaa
     5041 ggtagaactg cacgatataa aaaagccatc attactctta aagaaggcga aaaaattgaa
     5101 ctttttgagc aagtttagca tagaaaacag gattatataa tgggaattaa aaaacttaaa
     5161 ccaactactc caggctctag atttagatct aactctactt ttgaggagat tactaaaact
     5221 acgcctgaaa aatctcttac cggcgtaatt aaaaaatccg gcggaagaaa taatcttgga
     5281 catattacaa caagatttat tggtggtgga cataaaagaa gatatagaat tattgatttc
     5341 aagagaaaca aaccaggaat tcctgcaaaa gttttctcta tcgaatatga tccgaacaga
     5401 acttgcagaa tagctttgct gcattatgct gacggtgaaa aaagatatat tcttgcccct
     5461 gaaggtttaa aagttggaag taaaatagtt tcaggccctg gtagtgatat aataattggc
     5521 aacgctcttc cattaaaaga aatgcctctc ggaagttttg ttcataacgt tgaaatgaaa
     5581 ccaggtaaag gcggacagtt gggaagaagc gccggagcat cactacaggt tatggctaaa
     5641 gaaggagact ttgcacaact taaaatgcca tcaggtgaag taagattgat aagattagag
     5701 tgcatggcaa cttatggtgt tgttggtaat tccgagcatg aaaatttaag tcagggtaaa
     5761 gccggtagaa acagatggag aggaattaga ccaagtgtta gaggtgttgc gatgaaccca
     5821 gttgatcacc caatgggtgg tggtgaaggt aaaacttcag gcggcggtca tccggtttct
     5881 ccttggggac aaaaagcaaa aggtttgaaa accagaaaaa gaaaaaaaga atctaataaa
     5941 cacattatta aaagacgcaa gtagtaatta gagagtaact tatgccaaga tccgtaaaaa
     6001 aaggtccttt tctagattat aaattagctg agaaaattaa aaagctaaac gatactaatc
     6061 agaaaaaaat tattaagacc tggtcacgtt catcaactat ttttccagat tttgttggac
     6121 atacaattgc agttcataat ggtaataaaa tgattcctgt ttatgttact gaaaatatgg
     6181 ttggtcataa gcttggtgaa tttgcaccta caagaatatt cagaggtcat cctggtacaa
     6241 aagcagaaaa agcatctaaa ggataaataa taggatttga aattaatgga agctagagca
     6301 ataaacagat atataggttc atcaccacga aaaatgagat tagttattga tcttatcaga
     6361 ggaatgcgtg ttgataaagc tttggaagtt ttacactttt catcaaagca ttcgtcaaaa
     6421 gatgctgaga aaactttaag atcagccgtt gccaatttac ttaattctga tgaggctacg
     6481 cgagttgaac cacaagattt gtatgttaaa gaagtatttg tagatcaggg acctacaatt
     6541 aaaagaattt caccggcacc aatgggacgt gcatacagaa tcagaaaaag atcctgtcat
     6601 ctaactgtca ttgttgcaag taaagtataa ttaaggagat agatttttgg gacaaaaaac
     6661 acaccccgta ggttttagag ttggagtcat cagaggttgg gattctaact ggtatgaaaa
     6721 taaaagttat gcattcaaat taaaagagga tgacaaactt cgtacttata taagaagcag
     6781 attaaagaag gcgggaatat caagaatcgt tattgatagg acatcgaaga atattatttt
     6841 aacaattcat acttcaagac ccggagttgt tattggtaaa agtggtaaag aaattactca
     6901 gcttgaagaa gaattaaaaa gagttacaag taaagatgta aaagtccaga tatcagaaat
     6961 caaaagacct gaattagacg cttacctggt tgcagaaaat attgcaaatc agcttgccgg
     7021 aagagtttct tttagaagag ctatgaaaac ttctataact gctgcaatga gaatgggtgc
     7081 tgaaggtata agaattatgt gcgccggaag acttggcggt gcggaaatgg ccagaactga
     7141 acagtataaa gagggaagga ttccgcttca tacaattaga gctgatatag attacgcaaa
     7201 tggtagagcc gaaacaatat atggttctat tggaattaaa gtttggattt gccgtggaga
     7261 gattcttggc aaaagagtta ctgaataaaa tcgcttagga gtttttttct tatgttaatg
     7321 ccaaaaagag ttaagtatag aaaatcgcag cgcggaagaa gagccggtaa agcttatcga
     7381 ggtagttccg ttgcatttgg agattttggt ttaaaagcta tggaaccagc ctggattaca
     7441 agcaggcaga ttgaggcttg tcgtgttgct cttaccagaa aaatgaaaag agatggtaag
     7501 gtttggataa gaatatttcc agataaaccg gtttctaaaa aaccgcttga aacaagaatg
     7561 ggtaaaggaa aaggagcacc agagttttgg gttgctgtta ttaaacctgg aagagtaatg
     7621 tttgaagtag ctggtatctc aaaagagttg gcaagtgaag ctttaaaatt atgctcacat
     7681 aaacttccta ttaaaactaa agttgtaagc agacctgata tagaataaaa agagaatcat
     7741 agaaaatgaa attatacgaa ataaaagaaa tgtcaagtga agagcttgtt aagagaatag
     7801 aagaagaaga aaaaaactta attgatcttc gtttctcgca tcaacttaaa cagttgaata
     7861 acacttctaa acttgctctt gctaaaaaag atatagctag aatgaaaact attttgactg
     7921 aaagacagaa agcagacaaa gcagctcaaa cagataaaaa ggaaggagct aaagcttaaa
     7981 agatggaaca acgtacttta agaaaaacca gaatcggtgt tgtcgtaagc aataaaatgg
     8041 ataaaactat tacggttgct atcgaaagaa aagttcctca tcctatctac aaaaagtatt
     8101 ttaagaaaac tactaagttg atggctcatg atgaaaagaa ggaatgtggt ttaggtgata
     8161 aagttaaaat tatggaaact cgccccctta gcaagaataa gagatggcgc cttgtagaaa
     8221 ttgttgaaaa agccaagtaa ggagatttta taaaatgatt caagaagaaa ctaatttaat
     8281 tgttgcggat aattccggcg caaagcgagt aagatgtatc cgtgtgcttg gcggtagtga
     8341 tagaaaatat gctactgttg gtgataccat agttgtgtca gtaaaaaccg caattccaaa
     8401 tggtacggtt aaaaaaggtg aagtttctcg cgcagtgatt gttagaacta aaaaagaagt
     8461 tagaagaaaa gacggatcat atataagatt tgatgaaaat gctgcagtgt taattaatgc
     8521 tcagaatgaa cccaaaggaa ctcgtatttt tggccctgtt gcaagagagt tgagagagag
     8581 acagtttatg aaaatagttt ctttagcacc agaagttata taaggatttt ggataaatga
     8641 aaattagaaa aaatgatagt gtgatggtta ttgccggaaa tgataaaggc aaaaccggaa
     8701 aagttttaaa agtatttcct aaaacttctc gtattattat tgaggggatt aatcttagga
     8761 aaaggcatac aaagcctact caaaaatcac cacaaggtgg aatccaggaa aaagaagcag
     8821 cgatacattc ttcaaatgtt atgattcttg atcctaaatc gaacgaacca actaaaattg
     8881 gtgcaagaat tattttagat gaaaaatcag gtaaaaagaa aattgccaga gttagtaaaa
     8941 tttctggtga aatgattcct taagtaaatt taatttatta gagttgtata gaaatggctg
     9001 aaaagaaaac caaaaaatca gataaagaac cgcagaaaaa aggatctaca aaagaagcac
     9061 aatctgcgca agaaaaagaa attaagatca caccgagact tggtgagatc tacaaaaatg
     9121 atatagttcc tgctttagta aaaactttta gttataaaag tgtaatgcag gttcctaagc
     9181 ttgacaaaat agttgttaac atgggtgtgg gtgctgctgt agctgattca aaaattatgg
     9241 atgaagcagt taaagaatta gaaacaatta ctggtcaaaa agtaagtgta agaaaatcga
     9301 agaaagctat ttcaaacttt aagttacgcg aaaacatgaa tattggtgca atggtaactt
     9361 taagaaaaga aagaatgtat gagtttcttg acagactgat taacgttgct ttacctcgtg
     9421 ttagagactt taagggtctt tcggataaat catttgatgg ccgaggaaat tatactttag
     9481 gtgttaaaga acaaattatt tttccggaaa ttaatgttga caaaattaca agagttatgg
     9541 gtatggatgt tactcttgtt acaactgcaa aaactgataa cgaggctttt gagttattga
     9601 aagcattcgg aattccattt agaaaaaaag aagttaaata aggatttaaa ggagatttaa
     9661 tggcaagact tagcataatt gcccgtgatg caaaaagaag aaagcttaat gagaagtaca
     9721 aaaagcttag agaagaatta aaagaaaaag gtgattacga tgcattgcaa aagctgcccc
     9781 gcaactcatc tccggttagg ttaaaaaatc gttgcatgtt tactggtaga ggaagatcat
     9841 actaccgtaa atttggcgta tctagattag tcctcagaga gatggctctc agaggtgaga
     9901 ttccaggatt aaaaaaatca agttggtagg agttaataat tatgtccgtt actgatccga
     9961 tttcagattt tttgctaaga attagaaatg cttctaaggc aaagaaatta agagtagaaa
    10021 ttcctgcttc aaaaatgaag gtgggattgg ctgagatttt aaagaaagaa agttttattc
    10081 acgattattc tatagttgaa gataacaaac aaaacgttat taagatccag ctaaagtatc
    10141 gtgatggctc aagcgcaata accggaatga aaagaataag taaacccgga ttacgtgttt
    10201 ataaagatgc atcacaactg ccaagagttc ttaatggttt aggaactgct gtaatttcaa
    10261 cttctaaagg gctgctgaca gacaaagaag ctagaagtca atctgcaggc ggtgaagtaa
    10321 tttgttatat ctggtaatta ttttgatgga gatttaaaag tgtcaagaat tggaaaaaaa
    10381 ataattgaag tcccaaaagg tgttactgtt actttgggtg gaaatattgt aaaagtaaaa
    10441 ggcccaaaag gtgagcttca aagagcatgt catccaaaca tgaaaattga aatgaaagat
    10501 gctcagattg aagtaaaacg accggatgat ctgaaacaaa acagagcatt acacggatta
    10561 actcgcgcat tgattcaaaa tatgattctt ggcgttactg aagagtataa aaaagattta
    10621 gatattgtcg gagttggata taaggttgaa ttaaaaggaa caaacctttt acttaacatt
    10681 gggtactcac atccaatata ttttatacct ccaaccggaa ttaagattga agctacaacc
    10741 ccgactcata ttattgtttc aggtattgat aaacaattag ttggtcaggt tgcagcaaaa
    10801 ttaagatcga taagaaaacc agaaccttat aaaggcaaag gtattaaata ctctaacgag
    10861 cagattgtaa gaaaagccgg caagacagca ggtaaataat tctaatggag tttagactaa
    10921 aatgattaag cgaaatacaa attcaagatt aagatcaaag acaagaataa gaaagaaaat
    10981 ttctggaaca acagaagtac ctagacttac tatttacaga agtcttaatc aggtttatgc
    11041 acaaataata gacgattcaa ccggtagcac attagtttct gcatcttcat tatcaaaaga
    11101 acttgctgat gaaattaaaa actcaaaagg caaagtttca aaaagtaagc ttgtaggaag
    11161 tttagtggca aagaaagctt tggagcaaaa aatatccaaa gtagtttttg atcgtaatgg
    11221 ttatagatat cacggacggg ttcaagccat tgctgaaggc gctcgtgaag ccggattaaa
    11281 attttgataa ttgccggatc gtctaatggt aggactacgc cctttggagg cgtctgtaga
    11341 ggttcgaatc ctcttccggc aaccaaagtg ttaagaaaaa atttatttaa ttaaagattg
    11401 tttggagatt tatttgaaag ctgtaaaatc aactgatact gaaggactta aagaaaaagt
    11461 tgttcacatt aacagagtgg ctaaagttgt taaaggtgga cgtagattca gcttcaatgc
    11521 cattgttgtt gttggtaacg gaaatggtgt tgttggagta ggtcttggta aagctaacga
    11581 agttaccgat gcaatttcta aaggaattga tgatgcaaag aaaaacgttg ttaaagtttc
    11641 tcttataaaa ggaactgtag ctcacgagat tataggaaag tttggagccg ggaaagtttt
    11701 attaaaacca gcttcaccag gtacaggatt aattgcaggc ggcggagtaa gagcagtttt
    11761 agaagctgct ggtataactg atattcttac aaagtcactt ggctcaagca atccacacaa
    11821 tcaggttaaa gcaacacttg atggtctgtt aaaacagatt gatgcaagaa aaatggcttc
    11881 aaagagaggc attactatta aagatttgtt tattcgctcg aactgaggat aagaaatggc
    11941 aaaaattaaa attactcaaa caagaagtat tattgataga cctaaaaccc agaaagcaac
    12001 tattgaagct ttaggattag gaagaccaaa ttgggtaaaa attcataacg atactcctca
    12061 gataagagga atggtaaaaa aagtaattca cttggttaaa gtagaagaat taaaagatta
    12121 gtaaggtttt ataatggata ttttaagcaa tcttaaatac gcagcaggtt caaagaaaaa
    12181 tcgtaaaaga gttggacgtg gtgaaggttc tggacacggt aatcaatcta ccagaggtat
    12241 gaatggccag atgtcaagat cgggcgcaaa gcataaagcc tggtttgaag gtggtcaaat
    12301 gccgctgcag agaagagttc ccaaattcgg atttacaaat atttttaaag ttgaatatca
    12361 ggttgttaat ttaatttcgt tgcaaaaatt agttgatgca aaagttatta aagatcagac
    12421 actaaacgct gaagagttaa agaaattagg attagtttcc agctcaaaga aaccaattaa
    12481 ggttttgggc aaaggagatg ttaagtctaa attgaactta gctgttagtg atttcagtaa
    12541 atcagctcag caaaaaattg aagcagctgg tggaactata accaaagtat agcataaaat
    12601 agatgtcaaa atttacagat acattcagaa acatttttaa gattcacgaa cttagacagc
    12661 gtattttata cactttagct ttgctgttta tagttaggtt aggatcacat ttaaccattc
    12721 ccggtgttga tgcaacattg cttgcacaaa gtatgaagaa ttcttcatcc gataatcttt
    12781 ttggattgta cgatcttttt gttggcggtg ctttttcaaa tgcagcaatt tttgcattag
    12841 gaattatgcc gtacatctca gcatcaatta ttcttcagtt gatgggcgct gttgttcctt
    12901 atttccagaa acttcagcaa gaaggtgaag aaggaagaaa aaagattact cagttaaccc
    12961 gttatggaac ggtacttata tcagcaatgc aggcttgggg cgttactgta aaattgttaa
    13021 acactcagtt tcagggttta tcaattgttc ctcagcctgt gcaaggtatc ggctgggtat
    13081 tatctacaat aattatcctg acttccggaa caatgtttat gatgtggatg ggtgagcaga
    13141 ttactgataa aggaattggt aatggtattt cattgataat ttttattgga attattgcac
    13201 gttttcccca ttccattttt gatgagatta gactgatttc agccggacaa agatctatta
    13261 ttattgaaat aataatcttt gcttttatgt tctttattat tgcaggtgtt gtacttgtta
    13321 ctcagggaac aagaaaaatt cctgttcagt atgctaagag agttgttgga aggaaagttt
    13381 atggtggtgt aactcaatac attccactaa gagtaaatac tgcaggagta atgcccataa
    13441 tatttgctca atcaattatg tttatcccaa ctactgttct atcttttttc cctgataatg
    13501 aatttttgca attggtagga ggttggtttc aatatagatc accaatttat tctactgtgt
    13561 atgctttgat gattattttc tttacatact tttataccgc aattgctttt aatcccaaag
    13621 acgttgcaga taatatgaaa aagcaaggtg gatttattcc tggaataaga cccggaaaac
    13681 aaacatctga gtttattgat aatattttaa caaagatcac tttacctgga tcagtattct
    13741 tagcaatagt tgcaatatta ccagcatttg taagtgcatg gggagtctcg ggatcatttg
    13801 ctcaattttt tggtggaaca agtttattga ttattgtagg tgttgcacta gatactttac
    13861 agcaaattga atctcacttg ttgatgaggc attatgatgg ttttatgaaa tcaggtaaga
    13921 taagaggtcg cagataagat tttgatttta ataaaatcaa aaaaagagat tgattacata
    13981 agagaaagct gcagaattgt tgcagaaaca cttcagcttc ttaaatcaaa tgttagattg
    14041 gggattacaa ctttagagtt ggataaaatt gctgaagatt atattagaag caataatgca
    14101 ataccagcat tcaaaggtta ttcgcagggt ggtgctccgg gatttcccgg ctcgatttgt
    14161 tcttcagttg atgatgaagt tgtgcatggg attccaggtt caagagtact aaaagacggg
    14221 gagattatct ctttggatat tggagtcatt aaggatggtt attttggaga tgcagcctta
    14281 actgttgcag taggaaacat ctctgatgaa aagaaaaagc tgatggaagt tactgatcgt
    14341 tctttacaac ttggaattga gcaagcggtt gctggaaaca aagtccacga tatttcaaat
    14401 gctgtgcagg tttatgttga ggcaaatgga ttttcaattg ttagagatct ttgcggacac
    14461 ggtgtcggaa aatatctaca tgaagatcct tccataccaa actttggcag aaaaggaaca
    14521 ggaccgaagt tgaagaatgg gatgacatta gcaattgaac cgatggtaaa tgctggtaaa
    14581 tatgatgtgg ttacagcaaa agacggttgg acaattttaa cagcagatgg ttcaacttca
    14641 gcacattttg aacatacaat tttgataaat aataattcgc cggaaatatt aacagtttgt
    14701 taaatggcta aacaaggtcc gattaaaata gatggtataa ttacagaaac attaccaaat
    14761 gctacgttta gggtaaaact tgataacgga catgaaatcc atgctcatat atccggaaaa
    14821 atgagaatgc actttataaa aattttagta ggagataaag tttcaattga actttctcct
    14881 tatgatctga ctaagggcag aattacttac aggtataaat aggaataatt aaaatgaaag
    14941 ttagagcatc tgttaagaag atttgcgata aatgtaaagt tattaaacgc aagggtgttg
    15001 taagagttat ttgcaaaaac ccaaaacaca aacaacgtca aggttaaaaa caaaggagta
    15061 tatcattggc tcgtatagct ggagttgatc taccaaaaaa taaaaaagtg ttgtatggtt
    15121 tacagtacat ctatggaata ggtgagcata ctgcctcaac aattttagaa aaagctaaag
    15181 ttagtcccgt taaaaaagtt tccgatctta ctgaagaaga ggttgctgaa attagatcgg
    15241 tgataactgc tgaatacaga gtagaaggag ccttgcgttc agaagttcag cagaatatta
    15301 agagattaat ggatatcggt tcttatcgcg gtgtaagaca tcgtaagagt cttcctgcac
    15361 gtgggcagag aacaagaact aattccagaa ccagaaaagg caaaagaaga acagttgccg
    15421 gtaagaaaaa agcaccttct aagaaataac atttgataat acggagattt aagaattggc
    15481 taaaactgta aaaaaagtta agaaaaaaac acacgttgat tctaacggtg ttgcacatat
    15541 aaaagcaact tttaacaacg ttcttgttac tattacagat atttatggaa acacggtttc
    15601 ctggtcatct gcaggtaaga acggttttaa aggatcaaga aaaaatacac catttgccgc
    15661 acaggtttca gcagaagcag ccgcaaaaga ggcgtatgat ttaggaatga gaaaaatcga
    15721 agtttttgtt aagggacctg gttccggaag agaagcagcg ataagagctt tacacactgc
    15781 tgggttaacg atttcatcta ttaaagacgt tacacctatt cctcacaacg gatgcagacc
    15841 acctaagaaa agaagagttt aaaatattgg agattcacta aatggcaaga tacactgatt
    15901 cagtttgtaa attatgtaga agagaaagac aaaagctttt ccttaaagga cagaaatgtt
    15961 atacggaaaa atgtcctatc gaagaaagaa actatcctcc aggacagcac ggattatcta
    16021 gaagagctaa ggtatcagaa tacggagttc agttaaggga aaagcaaaaa attaaaagat
    16081 catatggtct gcttgagact caatttagaa attattttga aaaagcaacc agtcaaaaag
    16141 gtagaacagg cgaaaacctt attaaacttt tggaaagacg acttgatact gttgtttaca
    16201 gacttggatt tgcatcatcc agaaagcaag ccagacagct aattacgcac agacatataa
    16261 ttgttaataa tgttcctgtt gatattccag cttatttatt aaaagctggt gacgttattc
    16321 aggtaaaaga aaaaagtaag aaattagatg caattcacaa ttcacttaag agagtaaaag
    16381 ataacacata cggttggctt actgttgata aagccacact ttccggtacc tttgtacaaa
    16441 ttccggaaag agcagatgtt ccattgaatg ctaatgaaca attagtagtt gagctttact
    16501 cgaaataaaa tttttaatta gaataagata gaggataata atgagtgtat catctttaaa
    16561 gatgccggaa gctgttgttc ttgatgaagc caattacacg aactcgtttg gaagatttac
    16621 attgcagcct cttgaaagag gttacggtgt tactttgggt aattcacttc gcagggtgct
    16681 tttatcatcc ctgcaaggag cagcaataac ttcagttaag tttagcggag ttttgcatga
    16741 gtttactaca attgaaggag ttgtagaaga tgtttctgag attattttaa atctcaaaca
    16801 agttagaatg aaatttcttt ctaaaaaacc aaacaagatt gatatttcat tatccggaca
    16861 gggtgagtgg actgcggctg atattcagaa ggcaagtaat gaagttgaaa tattaaatcc
    16921 tgaattacat atcgcaacac taaacaaaag tgctaaactt gatattgaaa ttcgggttgg
    16981 taaaggtgtt ggctatgttc cggctcaaga aaatatagtt ccggatcaga caattggtgt
    17041 tattccaatt gattcaattt tcactccaat taagttagtt aagtatgatg ttgagaatgt
    17101 tagaattggt gataaaaatg attttgaaaa attaacttta gaaatcacta ctgatggttc
    17161 aattacacct gatgatgctc ttacgc
//
LOCUS       DASWBY010000052         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_378086, whole genome shotgun sequence.
ACCESSION   DASWBY010000052 DASWBY010000000
VERSION     DASWBY010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378086"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..495)
                     /locus_tag="VF870_01095"
     CDS             complement(145..495)
                     /locus_tag="VF870_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEX9250801.1"
                     /translation="MNKIIVYEKPTCTTCRKVSKLLYEQGVDFEKVNYYIEPFTKSKL
                     KDLLIKMKMKPSELLRKNDIVYKKLRLEIEKLSEDEVLDMMVINPDLVQRPMIEKGNK
                     AILARPPEKITELF"
     gene            656..>3260
                     /locus_tag="VF870_01100"
     CDS             656..>3260
                     /locus_tag="VF870_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9250802.1"
                     /translation="MKYFLALVFTLTLLITVNAQNFKQVKIYIQSLQDIAELQNAGLE
                     FDHPQITKENAVIVFIDDNDFQKLQMTNFRYEVQIDDWFEYYSKQPQLTESEKASLIQ
                     QSKDNYGVEGFGFGSMGGFYTLAEVISKIDSMRLLYPTLITSKVSIGNSVENRPIYMV
                     KISDNADIDENEPEILYTALHHAREPQSMMQMIYFMYYLLENYNTNPSVKYLVDNREM
                     YFIPVLNVDGYEYNRTTYPGGGGMWRKNRKNNGGSYGIDLNRNYGPYTYWNAPNGGSS
                     TDPASDTYRGPAPFSEPETAAVRDFLTTRYFKNALNYHTYSNLLIYPYGALEHETPDS
                     AAFREFARDMTAYNGYTYGLDQQTVGYSTRGNSDDYFYDGDILLNHGKILAMTPEVGS
                     SGFWPSQAEIFPLAIENLMPNLYYAYVAGEYVTAINPYFSQEFFNPGDNVELIVPQLK
                     NKGLSNASNISLSLSSDNPLVTITSGPINIGDIAARTSVNNNQNFSFTIGSAMPADVN
                     VKMLLTISTAGTPMFTDTLSFITGTPIMVFADTTNDPLLLWNITATPTTPKWEATNLS
                     FHSSPTSFTDSKTGNYLSNATVTMTLKNAIDLSANAHPRLNFWTKYDIESGWDYGQVE
                     VSTNNGTTFTPLSGIYTKSGSGSFQPNGEPLYDGVQSNWVNEVMDLTNYNTDQVKLKF
                     ELKTDNSVTKDGWYVDDIAVIIYGIIPVELSSFTAIAQDEIVMLNWVTSTETNNMGFD
                     IERRSTASNSGWQKLGFVSGKGTTTEKSAYSFADNNPVEGKSYYRLKQIDFDGSSKIY
                     NSVEVDFGVVKEYSLSQNYPNPFNPGTEINFSLAKSGNITLKVYNLLGSEVATLANGF
                     MESGKHSVKFNA"
BASE COUNT         1074 a          551 c          562 g         1073 t
ORIGIN      
        1 ctcataatca aacagacaaa ttatgatgat tattctcttt tactgtgcaa ttgttattgt
       61 gggatagctt agaaaccaga tgtataaaat tattcccatt agtaaggggg gttactaaga
      121 attattatgc tcacataacg ttcattaaaa taattctgtt atcttttcag gcggcctggc
      181 aagaattgct ttattaccct tctcaatcat tggtctttga accagatcag gatttattac
      241 catcatatcc agtacctcat cttcacttaa cttttcaatt tccaatctta actttttata
      301 aacaatatca tttttcctta acaactcaga aggcttcatt ttcattttta ttaaaagatc
      361 tttaagctta ctttttgtaa aaggctctat gtaataattg actttttcaa agtctacccc
      421 ttgctcatag agtagtttag aaacttttct gcaagttgtg catgtcggtt tctcataaac
      481 tattattttg ttcatatagt tttccttttc tgttgattca atcacaatgg ttgaaattac
      541 aaatcaattt ttgtacaaca tcttaaataa aaaaattatg tttatctaca aataatttaa
      601 ataaaacttg taatttggag ctaatcaaaa acattatttt ctaaaaaggt accatatgaa
      661 gtatttttta gctctcgtgt ttacacttac attattaata accgtaaatg cgcaaaactt
      721 taaacaagta aaaatatata ttcaaagtct gcaagatatt gctgaactgc aaaatgcagg
      781 attagaattt gatcatcctc agattacaaa ggaaaatgct gtaattgttt ttattgatga
      841 caatgatttt cagaaacttc aaatgacaaa ctttaggtat gaagttcaga tagatgactg
      901 gtttgaatat tattctaaac agccacagtt aactgaatct gaaaaagctt cacttataca
      961 gcagagcaaa gataattatg gtgttgaggg atttggtttc ggctcaatgg gtggatttta
     1021 cacacttgct gaagttatat ccaaaattga tagtatgcga ttactttacc cgactcttat
     1081 aactagtaaa gtttcaattg gtaattcggt tgaaaacaga cctatctata tggtaaaaat
     1141 ttctgacaat gcagatattg atgaaaacga accggaaata ctttatactg ctcttcatca
     1201 tgcacgtgaa cctcaaagta tgatgcagat gatttatttt atgtattacc tgctggaaaa
     1261 ctataataca aatccatctg ttaaatatct cgttgataat cgggaaatgt attttattcc
     1321 cgtacttaat gttgatggat atgaatataa ccgcactaca tatcctggcg gaggcgggat
     1381 gtggagaaag aaccgtaaaa ataatggtgg aagttatgga atagatttaa acagaaacta
     1441 tggaccttac acttactgga atgcaccaaa cggaggctca agtactgatc cggcaagtga
     1501 tacttacaga gggccagctc ctttttcaga acctgagact gctgcggtaa gggattttct
     1561 tacaacaaga tattttaaaa atgcacttaa ctatcacaca tacagtaatt tattaattta
     1621 tccttatggg gcgcttgagc acgaaactcc tgattctgca gccttccgtg agtttgcacg
     1681 ggatatgact gcttataacg gatatactta tggactcgat cagcagactg ttggatacag
     1741 cacacgcggt aacagcgatg attattttta tgatggtgat attcttctta accacggaaa
     1801 aatacttgca atgacacccg aagttggcag ttcaggtttt tggccaagtc aggctgaaat
     1861 atttcctttg gcaatcgaaa atcttatgcc gaatttatac tatgcgtatg tagcaggaga
     1921 atatgttaca gctataaacc cttatttctc acaggagttc tttaatcccg gcgataatgt
     1981 tgaactcata gtaccacaat taaaaaataa aggtctaagt aatgcctcta acatttctct
     2041 ctctctaagt tctgataatc cgttagttac aattacatct ggaccaatta atattggtga
     2101 tatagctgca cgtacttctg ttaataacaa tcagaacttc tcctttacaa ttggaagtgc
     2161 tatgcctgca gatgtaaatg ttaagatgct tcttactatc tcaacagctg gcacaccaat
     2221 gtttacggat acacttagtt ttattactgg tacaccaatt atggtttttg cggatacaac
     2281 aaatgatcct ttgcttttat ggaatattac cgcaacacca acaacaccaa agtgggaagc
     2341 aacaaattta agttttcact cttcgcccac ttcttttaca gatagtaaaa ccggtaatta
     2401 tttaagcaat gcaactgtaa ctatgacatt aaagaatgcg attgatcttt ctgctaatgc
     2461 tcacccaaga ttaaattttt ggactaagta tgatattgaa agcggttggg attacgggca
     2521 ggtcgaagtt tcaactaata atggaacaac atttactccg ctttccggta tttatacaaa
     2581 atcaggcagc ggatcttttc aacctaatgg tgagcctctt tacgatggcg tacaatcaaa
     2641 ctgggtaaat gaagtaatgg atttaacaaa ttataacaca gatcaggtta aacttaagtt
     2701 tgagttgaag actgataatt ctgttacaaa agatggatgg tatgttgatg atattgctgt
     2761 tattatttat ggcattattc cagttgagtt aagttcgttt acagcaatag cccaggatga
     2821 aatagtaatg ttgaactggg ttacatctac agaaacaaat aatatgggat ttgacattga
     2881 aagaagatca acagcatcaa attccggctg gcaaaaatta ggatttgtaa gtggtaaagg
     2941 gacaacaaca gaaaaatctg cttactcttt tgctgataac aatcccgttg aagggaaatc
     3001 atattataga ttaaagcaaa tagattttga tggttcatca aaaatttata actcagttga
     3061 agtggatttt ggagttgtta aggagtattc gttatctcag aattatccca atccatttaa
     3121 cccaggtact gaaattaatt tctcgcttgc taagtcagga aacataactt taaaagttta
     3181 caaccttctt ggttcagaag ttgcaactct tgcaaatgga tttatggaat caggaaagca
     3241 ctccgtaaag tttaatgctt
//
LOCUS       DASWBY010000053         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_566309, whole genome shotgun sequence.
ACCESSION   DASWBY010000053 DASWBY010000000
VERSION     DASWBY010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566309"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VF870_01105"
     CDS             complement(<1..188)
                     /locus_tag="VF870_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250803.1"
                     /translation="MSTFTIVGIAQNHVPSFEEVISLHSVGTVEISADGKSVAFTVQK
                     TDWNENRYDTEIWLSKDG"
     gene            complement(1018..1272)
                     /locus_tag="VF870_01110"
     CDS             complement(1018..1272)
                     /locus_tag="VF870_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250804.1"
                     /translation="MLVLFFVTSCAPVGMTKEEYGFFYGIVHGFISPFVLIAKLLGAH
                     IGLYAENNAGTLYWLGFILGVVLLLGGGGGSYATRSRWYD"
     gene            complement(1458..1661)
                     /locus_tag="VF870_01115"
     CDS             complement(1458..1661)
                     /locus_tag="VF870_01115"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX9250805.1"
                     /translation="MAVLKVIEIMTSSPKSWEDATTIAVKTAGETVKGIRSVYIHHLS
                     ALVEDNKISEFRVNAKITFEVMN"
BASE COUNT          573 a          349 c          282 g          561 t
ORIGIN      
        1 tttccatctt ttgaaagcca gatctcagta tcatagcgat tttcgttcca atcagtcttc
       61 tgaacagtaa aggcaactga tttgccatct gctgagattt ctacggtgcc tacactatgc
      121 aaagaaatga cctcttcaaa agaaggtacg tgattctgag caatcccaac gattgtgaat
      181 gtgctcaaaa gcacaagcag taattttttc ataacctcgt ttttataaat gggtaaaagt
      241 agaatgtact atggttcatt atcaaaatta aaccattgcc aggctaaatg caaaactttc
      301 ttcttaaggc agaaaatcat tataaaacct ctgcaggtca actaattcca ccatgtctct
      361 gattaagctt aggttagcta tttcaaacat cttgtctaca aagaggatca tgaaataatc
      421 ctccctccgt tactaataca aaataaaaac ccttgtaaat taataactta caaggatttc
      481 ttttttatcc agtgtctcat tcagtgtctt aatattaaat ctaacttctt aattaagaac
      541 tatttcggtc agttaacact ccaaggtgga gtaaaatatg gtggtgtata cttaataagt
      601 attgtgtcac tctttattgg gctttttgca ggagtggcac ttttaaatta atcccgtcct
      661 cctgtgcgat caggatgaca gctggttgac atagttttga actacatttt ttcatacatc
      721 tacttgaaag gccaaaacac ctaaattttg tccaaaacct ggcaccggac caaccgtaaa
      781 gaaattgccc tgtggcaatt ttaaggaggg gccagattgt atggcggggt ttgataccag
      841 ctgaaaagat tatgtgtcag agtttgattt tcttcaaaat ccgtccctct ctgacaactt
      901 caaaagcagg tctgataaca cttaacatac ataacaaaac gacaaggatt taactccaaa
      961 ttctggtggt caatatgatg cagccaacta tggataaggt cttttacgat ttctgcttta
     1021 gtcgtaccat ctacttcttg ttgcataact accgccacca ccgccaagaa gaaggacaac
     1081 acccaaaata aatcccaacc aatataacgt acctgcatta ttttcagcat acaatcctat
     1141 atgcgccccc aatagttttg ctatcaatac gaaaggggaa atgaatccgt gaactatacc
     1201 ataaaagaaa ccatactcct ccttggtcat gccaacagga gcacaggaag taacaaaaaa
     1261 cagaaccagc aataataata atccaataaa tattcttttt gttttcatat aaattaattt
     1321 aaaaatgttt ttactcgtta agtgattata atttaatgaa cagataagcc actcttctta
     1381 tggttacagc tttcaatact atagctgaaa gagtagccca taatagaaca atttatttga
     1441 ttagtacttt taaccggtta attcataacc tcaaaagtga tttttgcatt gacccggaat
     1501 tcagagattt tattatcttc cactaatgcg ctcaggtgat gaatgtaaac tgagcgtatt
     1561 ccttttacag tttcccctgc agttttgacg gcgattgttg ttgcatcctc ccagcttttg
     1621 ggcgatgatg tcatgatttc aataacttta aggacagcca tgatgtgata attttaaatt
     1681 aactatttga actttcgtat tttatgaatg cagaacctat tgcaattcca aatatcttat
     1741 tagttacata taataattca gatac
//
LOCUS       DASWBY010000054         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_2231, whole genome shotgun sequence.
ACCESSION   DASWBY010000054 DASWBY010000000
VERSION     DASWBY010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VF870_01120"
     CDS             <1..203
                     /locus_tag="VF870_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX9250806.1"
                     /translation="IEELIHDLKQKYTIVIVTHNMQQAARVSDMTAFFYIGELIEFDR
                     TTKIFTNPSIKQTEDYITGRFG"
     gene            276..1004
                     /gene="phoU"
                     /locus_tag="VF870_01125"
     CDS             276..1004
                     /gene="phoU"
                     /locus_tag="VF870_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX9250807.1"
                     /translation="MERLLDEHLEKLKTRVIKMCSLVDEQVQFAIKAVEDDNLELAQS
                     VIDRDKKVNKYDVKIDKICQKIFALAQPVAMDLRYIMSSLTINSNLERIGDIAVNISE
                     NILMIKKKPEFYHDTKLEEMFKLTKRMLKNSIDAFINGYPELAKEVILTDDIVDNLNA
                     ENHQILKSIMKKTPDNIESAVALLVISRELERLADHATNIAEDVFFIVEAQLIKHKYE
                     KFIFDEDEADSETDSDENKEGKIS"
     gene            complement(1084..1659)
                     /locus_tag="VF870_01130"
     CDS             complement(1084..1659)
                     /locus_tag="VF870_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9250808.1"
                     /translation="MANDRKQQIIKAAAKRFARHGLSKTTLDEVARDIRIGKATIYHY
                     FTSKDDLYFSVLRWECDLLIEQVKNIFENESGTINQKLSDYIGLKDIISENNKLIYEA
                     LLAFFYDKALDQETEVIKLLIVKESELIKHFLTKHFAQKIKKASSSLPYFIVVNSWGM
                     LFGNKLNSVIEPSKSAQTKELFIESLEAMLD"
     gene            complement(1727..>2650)
                     /locus_tag="VF870_01135"
     CDS             complement(1727..>2650)
                     /locus_tag="VF870_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250809.1"
                     /translation="ITQSRMGLLVNGLVKIPMQFIILFIGAMVFVFFQFVTPPLFFNP
                     VETKKVKAGSYSEEYSNLEKEFDNLQQIKRVQLRQMLDESSGSSLSSSVNENSAGIVK
                     KELDDLSKREQSIRTSAKEIIKKNNSEADTNDTNYIFITYIINYLPVGLIGLLLAAII
                     AASMSSTSAELNSLASTSLIDIYKRLINKNASDKKYLIFSKYATVGWGIYAIIFALLA
                     SELGSLIEAVNILGSLVYGTILGIFLVAFYTKKISGNSVFTSAIIAESVVLYCFLFTS
                     IPFLWYNVIGCLLVVFLSLALNPIFSQKEAN"
BASE COUNT          920 a          402 c          433 g          895 t
ORIGIN      
        1 aaattgagga gctgattcac gatttaaaac agaaatatac aattgtaatc gtgactcata
       61 atatgcagca ggcagccaga gttagtgata tgactgcatt tttctatatc ggtgagttaa
      121 tagaattcga tcgtacaacc aaaatattta ccaatccttc tattaaacaa acggaagact
      181 atataaccgg aagatttggt taataaattt tagtcattct gaacttgttt cagaatcaag
      241 taaagtttta ttttcatgga gattaaggag aacaaatgga aagattatta gacgagcatc
      301 ttgaaaaatt aaaaacacgc gttataaaaa tgtgcagcct tgttgatgaa caggttcagt
      361 ttgcaattaa agctgtggaa gatgataatt tagaattagc acaatctgtt attgatcgtg
      421 ataaaaaagt taacaagtat gatgttaaga ttgataagat ttgtcagaag atatttgcac
      481 tggctcagcc ggttgcaatg gacttaagat acattatgtc atcgcttacg ataaacagta
      541 atcttgaaag gattggtgat atagctgtta acatctctga gaatattttg atgattaaaa
      601 agaaaccgga attttatcac gatactaaac tcgaagaaat gtttaagctt acaaaacgaa
      661 tgttgaaaaa ttcaattgat gcttttatta acggttatcc ggaacttgct aaagaagtga
      721 ttttaactga tgatattgtt gataatttaa atgcagaaaa tcatcagata ttaaaatcta
      781 ttatgaagaa aactcctgat aatattgaat cagccgtggc attattagtt atctcgcggg
      841 agttggaaag attagcggat catgctacaa atattgctga agatgttttc tttatagttg
      901 aagcgcagct tattaaacat aaatatgaaa agtttatttt tgatgaagat gaagctgata
      961 gtgagaccga ctctgatgaa aataaagagg ggaaaatttc ttagagtttg atttttattc
     1021 tctttctatt tcattctgtt aaaagtatgt tcaccggcag gatagttgaa gcctggcata
     1081 tttctaatct aacattgcct ctaaagactc aatgaacaat tcttttgtct gagcagactt
     1141 tgaaggttca atcacagaat taagtttatt accaaacaac attccccagc tattaactac
     1201 aataaaatat ggcagagatg aactggcttt tttaattttt tgtgcaaaat gttttgtaag
     1261 aaaatgcttg attagttctg attctttgac gattaataat tttataacct cagtttcctg
     1321 atctaaggct ttatcataaa aaaaagctaa tagagcctca tagattagtt tgttattttc
     1381 ggaaataata tcctttaatc ctatataatc agataacttc tggttaattg ttcctgattc
     1441 attctcgaaa atatttttta cctgttctat taataaatcg cattcccatc tcagtacgga
     1501 aaaataaaga tcatcttttg aagtgaagta atgataaata gttgctttcc cgatacgaat
     1561 atcacgagca acttcatcga gtgtagtttt gcttaaaccg tggcgcgcaa atctttttgc
     1621 tgctgctttt attatctgtt gttttctatc gtttgccatg atttataaaa ttattcaatt
     1681 gaaaaataat gactagactt ttaacaagta agtatttgag agataattaa ttagcttctt
     1741 tttgtgaaaa gattggattg agtgccaggc tcaaaaacac aacaagtaaa cagccaatca
     1801 cattatacca taaaaaggga attgatgtga aaagaaaaca ataaagcact accgactctg
     1861 caattattgc acttgtaaac acagaattac ctgaaatctt ttttgtgtaa aatgcaacaa
     1921 gaaaaattcc aagtatcgtt ccgtaaacaa gcgatccaag aatatttact gcttcaatca
     1981 atgagcctaa ttcacttgct agtaaagcaa atatgatcgc ataaattccc cagccaactg
     2041 tggcatattt agaaaagata agatattttt tatcactagc atttttattt attagtcttt
     2101 tataaatatc aataagcgaa gtagatgcta atgaatttaa ttcagcagat gttgatgaca
     2161 tagaagcagc gataattgcc gccagtaata atccaattaa tccaacaggt agataattta
     2221 ttatgtatgt tatgaaaata taatttgtat cgtttgtatc ggcctcagaa ttatttttct
     2281 ttataatctc tttagcactt gttctaattg attgttctct ctttgataaa tcatccaatt
     2341 cttttttaac aatcccggct gaattttcat ttacacttga acttaaactt gaacctgaac
     2401 tttcatcaag catctgtctt aattgcactc gcttaatctg ttgcaaatta tcaaactctt
     2461 tttcaaggtt tgaatattct tcagaataag aaccggcttt tactttttta gtttcaacag
     2521 gattaaaaaa cagtggggga gttacaaatt gaaaaaacac aaataccatc gcaccgataa
     2581 atagaataat gaactgcatc ggaatcttta ccaagccgtt tacgagcaat cccattctgc
     2641 tttgtgtgat
//
LOCUS       DASWBY010000055         8104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_378185, whole genome shotgun sequence.
ACCESSION   DASWBY010000055 DASWBY010000000
VERSION     DASWBY010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378185"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VF870_01140"
     CDS             complement(<1..284)
                     /locus_tag="VF870_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250810.1"
                     /translation="MKRIQASFIIIILSFLFYSTTFAQLINIQRFIGRSQLDLINELG
                     QPVHFDNSNPSMMLMSYNLFSLSCIADQNGIYKVELKRSYTSEKAAYDDI"
     gene            complement(421..750)
                     /locus_tag="VF870_01145"
     CDS             complement(421..750)
                     /locus_tag="VF870_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250811.1"
                     /translation="MVLILWQLLSASLFNGYGVNEQCGRNRNNNTIKIINDIHSETLF
                     TIKDVKGTQNLFPYLEKQKYRHDTKYNLHQAKLLLIGCLIEKKEFYVNKVEKGNLNPR
                     SPPQYYI"
     gene            complement(847..2661)
                     /locus_tag="VF870_01150"
     CDS             complement(847..2661)
                     /locus_tag="VF870_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9250812.1"
                     /translation="MFKKIILGLILILLITNHLRAQDISAGVKLIRNEKFNSAKKYFY
                     SLLTTDKKAESYFYLGQIFYLTEDYDSAKISYLNGITADPEQPLNYAGLVKINLLSNE
                     ISEADKNIKNALELGNEKSPLVYITLSEAYSNSKVKDYSKASELISNAIKIEPTYIDA
                     YISLSNVDLLKGDGTEAIKNSEEILNIDNKNSEALTIKAEVYILINKNNEAIGLLNDA
                     IKYDSSYSPAFNELAELYADLKDYSKAAEYYEKYIQASEVTIEKQKRYASILYVNKEY
                     EKTINILEDVLRTENNSASTIRIIAYSYLRLGNVDKSKYYFEKLFQIKSAEFLPTDYE
                     NYADLLSKTGNEIQALDYLAKVIELDSTRKDIYGKMSVIYFKNKNWDGVISSLTEKGS
                     LTAQEYFDLSKAYIFKGDGNITNALQSLTSKVKMEIEQIDKTRQILLYYQGDLFKAKG
                     DTQKIVEAKSKTIQSVEALLNNNQKKDWNLAKDEWSNLIESTISTEYAKADSCLNILV
                     TKAPNLTIAYVWQARVKTDFDPDSENGLAKPFYEKFIQLANPESDKFKKELIESYSYL
                     GYYYYLQKDNSKAKSYWQEVLKLDPENKQANDVIKQLK"
     gene            complement(2673..3590)
                     /locus_tag="VF870_01155"
     CDS             complement(2673..3590)
                     /locus_tag="VF870_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9250813.1"
                     /translation="MKNSLIVLFFFLQLTVFFACNKQDSPDDTPTTGKLTICADETYK
                     PLIDSEIATFESIYKYSDISVKYKAETEAFNDLMNDSVRLIIVSRNLNKEELKQFEKW
                     EIVPKINKVAYDAIALIGTKDIKDSIYTISKINSLINSDVKDRKLKNTKVVFDNNNSS
                     TIRFIKEKTGVKNFSSNCYALSSSKAVLDYIAKEKNCIGIIGVNWISDHDDSTYISFL
                     KSVKVLEVAIDEKSESYKPYQAYIAQKTYPLYREMYIISKEAYTGLGTGFTAFVASER
                     GQRIVLKFGLVPATMPIRLVELINDDITN"
     gene            complement(3580..3783)
                     /locus_tag="VF870_01160"
     CDS             complement(3580..3783)
                     /locus_tag="VF870_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250814.1"
                     /translation="MTVKEISNIVMAIVYLGSGGFLVLGENIFNFSELQKIGLGVLLI
                     LYGLFRVYSFIKKLKERISKNEE"
     gene            complement(3861..4652)
                     /locus_tag="VF870_01165"
     CDS             complement(3861..4652)
                     /locus_tag="VF870_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX9250815.1"
                     /translation="MADQIVFKANIDELIFAKKNKEYGAYKLRKAYKTYLSAAMWIAI
                     LIVFFATTGPTIYRMLNPENVESKKLKKVIITDLAPPPSIGDEQKVEQIEAPPPLKST
                     IKFTPPVVKADEQVKDEYIPTVDELKTVDPGMKTQQGTEGGVDYSLIEVEEKVVEEKK
                     EEAPKYFVAVEEMPEPIGGIAAIQQLITYPEIAKRAGVEGKVYVLAFVDETGRVTGAK
                     IIKGIGAGCDEAALDAVRQTQFKPGKQRGTPVKVQVSIPIVFKLQ"
     gene            complement(4665..5192)
                     /locus_tag="VF870_01170"
     CDS             complement(4665..5192)
                     /locus_tag="VF870_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9250816.1"
                     /translation="MAELEQKQAGAKKGKKKGRKKMSTRIDLTPMVDLGFLLVTFFML
                     TTTFSKPQTMEINLPVKPKGEIKEEDQNALKASKAFNIILGKDNKLFWYIGLANEPID
                     GPNKTDYSADGIRKVLLEKNSTIKDMVVLIKPTDDAVYKNVVDILDEMNISNIKRYAL
                     VDIAKEELDLIKNLN"
     gene            complement(5196..5816)
                     /locus_tag="VF870_01175"
     CDS             complement(5196..5816)
                     /locus_tag="VF870_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9250817.1"
                     /translation="MPKVKIPRKSTLIDMTAMCDVAFLLLTFFMLTTQFKSDDSVIVD
                     TPSSISEIKLPDTDILNITITLDGKVFFSIDNKNFTREKLLAKISEKHPELQFDAAEQ
                     KAFVLTGSIGVPLNQLKGYLALDEADRKMYSQPGIPVDSINNELGDWIMQGRLSNPGV
                     RITVKGDQGCPWPIVKKVMNTLQDKNVNKFNLITDLEVDPNKLKGI"
     gene            complement(5847..6653)
                     /locus_tag="VF870_01180"
     CDS             complement(5847..6653)
                     /locus_tag="VF870_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9250818.1"
                     /translation="MKTKFELKSAFAGFAAIAIPVLFVVALVLYFFIMGNPENFQGNN
                     VNNLPKPGNYLGVIYKGGIIVPLLMTILMMVITFTIERFITITKAKGKGSIASFVVRV
                     YQKLSSQDIDKAIMECDGQRGSVANVLKAGLERYKSVTESSEMNKEQKIASIKQEIEE
                     ATSLELPILEQNLVILATIAPLATLMGLLGTVLGMIKAFSALATAGAPDAVALATGIS
                     EALINTAFGIGSSALAIIFYSYFTTKIDKMTYSIDEAGVAIVQNFSSHHK"
     gene            complement(6855..7538)
                     /locus_tag="VF870_01185"
     CDS             complement(6855..7538)
                     /locus_tag="VF870_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250819.1"
                     /translation="MTGLAYPTGGILETPEKLGSIDQTNLLKSGSSGKTIFGSAGSHA
                     QVNRSIPSANWVERGIQKMHIEHAIIRLNGKINLKFGQLFFDYFDLYLIREKKMLSTF
                     NKYIIRSSAIVLIIVTLLQLISSRLQFTSNDQKNPNNNETILVVSNQIQSSPCKTENN
                     VKGFDRSSGSETSLLKIIGQEKILYSEDVARNSNIKLLYTIKILNEINILKFTTNHLD
                     LRSPPIFFS"
     gene            complement(7619..7891)
                     /locus_tag="VF870_01190"
     CDS             complement(7619..7891)
                     /locus_tag="VF870_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX9250820.1"
                     /translation="MVNNFKNPKEYDDFTIIEVWRKAIPYKRFELYKKDCFGSIIFFD
                     DYLIETENGWIIDYIVPIEKGGTNEIENLRPVHWKNIGHISHNYGG"
     gene            complement(7907..>8104)
                     /locus_tag="VF870_01195"
     CDS             complement(7907..>8104)
                     /locus_tag="VF870_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX9250821.1"
                     /translation="PDNFAGKTLKELKLRSKYDVLVIGVNEILNDKYILMPEPDYKFK
                     PDVVITLLGRKSSLDNFRLSK"
BASE COUNT         2457 a         1406 c         1151 g         3090 t
ORIGIN      
        1 cttatatcat cataagctgc tttttcagat gtatagcttc tttttaattc tactttatat
       61 attccgtttt gatcagcaat acagcttagc gaaaataagt tataagacat caacatcatt
      121 gatggattgc tattatcaaa atggaccggt tgacctaact catttattaa atctaactga
      181 gatcttccaa taaatctttg aatgttaata agttgagcaa aagtagtact gtagaaaaga
      241 aacgacagaa ttatgattat aaaacttgct tgaatccttt tcatgatctt tactccttta
      301 atgatgcgac atagataaat ttaaacttcc tattattatt tttagactta attttttaat
      361 cccatattca aaagaatctg cttgcaaata gtagaatatt ttgaatgctt ttattaaaaa
      421 ttagatataa tattgaggag gacttcgggg gtttaaatta cctttttcaa ctttattaac
      481 ataaaattct tttttttcaa taaggcaccc tatcagcaat aacttagcct gatgcaagtt
      541 gtattttgta tcgtgcctat atttttgctt ctctaaataa ggaaataagt tttgtgttcc
      601 tttaacatct ttaatcgtaa ataaagtttc agaatgaata tcattaataa tcttaatagt
      661 attattattt ctgtttcttc cacattgttc attaactcca tacccattaa ataatgatgc
      721 acttaataac tgccagagta ttagcaccat tattaaaata atctttaaca aattttttat
      781 atggaatttt agattaaaca ttttttattg taataataac attttataac tataattcag
      841 caataatcat ttaagttgtt taattacgtc attggcctgt ttgttttccg gatctaattt
      901 taaaacttcc tgccagtaac ttttagcttt tgaattatcc ttctgcaagt agtaataata
      961 accgagatat gagtatgatt ctataagttc ttttttgaac ttatcgcttt caggatttgc
     1021 aagttgaatg aatttttcat aaaaaggttt tgctaatcca ttttcagaat cagggtcaaa
     1081 gtcagttttt actcttgcct gccatacata tgcgatagtt agatttggtg cttttgtaac
     1141 tagaatgttt aaacaagaat cagcttttgc atactcagtg ctaatcgttg attctattaa
     1201 attactccat tcgtctttag ccaggttcca atcttttttc tgattattat ttaacaatgc
     1261 ttccacagat tggatagttt tggatttagc ttcaactatt ttttgcgtgt cacctttagc
     1321 tttaaataaa tctccctggt agtatagaag tatttgtcgg gttttatcaa tttgttcaat
     1381 ctccattttt actttactag ttaaagattg taaagcatta gttatatttc catcgccctt
     1441 aaaaatgtag gccttactta aatcaaagta ttcctgcgct gttaaacttc ccttttctgt
     1501 taaagaactg ataactccat cccaattttt atttttgaaa taaataacgg acatttttcc
     1561 gtaaatatct tttcttgtac tatcgagttc gattactttt gccaggtaat ctaaagcttg
     1621 tatttcattt ccggtcttac ttagtaaatc ggcataattc tcataatctg tcggcaaaaa
     1681 ttctgcagat ttaatttgaa ataacttttc gaaatagtat ttacttttat caacattgcc
     1741 cagtcgtaaa taagagtatg caattatccg gatagtggat gcagaattat tttcagttct
     1801 taatacatct tcaagaatat taattgtctt ttcatactcc ttattcacat ataaaataga
     1861 cgcatatctt ttctgttttt caattgtaac ttcagatgcc tggatatatt tttcataata
     1921 ctctgctgct tttgaataat cctttaagtc tgcataaagt tctgctaatt cattaaatgc
     1981 tggtgagtat gaagagtcgt atttaattgc atcatttaat aatccaatcg cttcattatt
     2041 tttatttatt aggatgtaaa cttctgcttt aattgttagc gcttcggaat tcttgttatc
     2101 aatatttaaa atttcttcgc tgttttttat agcttcagtg ccatcccctt ttaataagtc
     2161 cacattgctt aagcttatat aggcatcaat gtatgttggt tcaattttaa tagcatttga
     2221 tatcagttcg gaggcttttg aataatcttt tacttttgag tttgaatatg cttcagacaa
     2281 tgtgatgtaa actaacggac ttttttcatt acccaattcc aatgcgtttt tgatattttt
     2341 atccgcttca gaaatttcat tcgaaagtaa attaattttt acaagccctg cataattaag
     2401 cggttgctca ggatcagccg ttattccgtt tagataagat atttttgcag aatcgtaatc
     2461 ctctgtaaga taaaatattt gcccaagata aaagtatgat tcagcttttt tatcagttgt
     2521 tagaagtgag taaaaatatt tttttgcgct attgaacttc tcattccgga ttagttttac
     2581 ccctgcggaa atatcctgag cccgtaaatg attagtaatc aataaaatta agattaatcc
     2641 cagtattatt ttcttaaaca tttttacacc atttaatttg ttatatcatc attaataagt
     2701 tctaccaatc ttattggcat tgttgcgggt acaagtccaa atttaagtac gattctttgt
     2761 cctctttcgc tggcaacaaa agcagtaaat ccggtcccca gtcccgtata agcctctttg
     2821 ctgattatat acatttcccg atacaatgga taagttttct gtgctatata agcctggtaa
     2881 ggtttatatg attctgattt ttcatctatt gccacttcca gaactttaac actctttaag
     2941 aaactaatat acgtcgaatc atcatgatca cttatccagt tcacacctat tattccaata
     3001 caattttttt ctttcgctat ataatcaagt acagccttac tagagctaag cgcataacaa
     3061 tttgatgaga aattttttac acctgttttc tcttttataa atcttattgt acttgaattg
     3121 ttattatcaa aaacgacttt tgtgttctta agttttcgat ccttaacatc tgaatttata
     3181 agagagttga tctttgaaat tgtatagata gaatctttaa tatcctttgt tcctattaat
     3241 gcaatcgcat cataagcgac tttattaatt tttggtacta tctcccattt ctcaaattgc
     3301 tttaattctt ctttattcag gtttcgcgaa actattatga gcctaacgct atcattcatt
     3361 aaatcattaa aggcttcagt ttctgctttg tactttacag aaatgtctga atatttataa
     3421 atagattcaa aggtcgcaat ctccgaatca atcagcggct tatatgtttc gtctgcacat
     3481 atagttaatt ttcctgtagt tggtgtgtca tccggactgt cttgtttatt acaggcaaag
     3541 aacactgtta gctgcaagaa aaaaaacagt actattaaac tattcttcat ttttactaat
     3601 tctttcttta agttttttaa taaaactgta cactcggaat aaaccataca gtattaacaa
     3661 aacacccaaa ccgatttttt gtaattcaga aaaattgaat atattttcgc ccagaacaag
     3721 aaagccacca cttcctaaat aaacaattgc cattacaata tttgaaattt ctttaacagt
     3781 catatcaaac agtcaattaa tattgttttt gtaaagtaca tattcaattt atcttcattg
     3841 atatactgaa cctcaaaaga ttattgtagt ttaaatacaa ttggtattga tacttgtact
     3901 tttacaggtg ttcctctttg cttacccggt ttaaattgcg tctgtctcac tgcatcaaga
     3961 gcagcttcat cacaaccggc accaatacct ttaataattt tagctccagt aacccttcct
     4021 gtctcatcaa cgaatgcaag cacataaact tttccttcta cgccggcacg cttagcaatt
     4081 tcaggataag ttattaattg ttgtattgct gctataccac ctataggttc aggcatttct
     4141 tcaacagcaa caaagtattt aggtgcttct tccttctttt cttcaacaac cttttcttca
     4201 acttcaatta aactgtaatc tactccgccc tcagtacctt gctgtgtttt cattcctgga
     4261 tcaacagttt ttaattcatc caccgttggg atatactcgt ctttgacctg ttcatcagct
     4321 tttacaacgg gaggagtaaa ttttattgtc gactttaatg gcggtggtgc ttcaatctgc
     4381 tcgactttct gttcatcacc aatagatggt ggtggtgcta aatctgttat tatgaccttt
     4441 tttaattttt ttgattcaac attttccgga ttaagcattc tataaatcgt tggtcctgtg
     4501 gtagcaaaga atacaattaa gattgcgatc cacatagctg ccgaaagata agttttatat
     4561 gctttgcgaa gcttgtaagc tccatattct ttattctttt ttgcaaatat caattcatcg
     4621 atattggctt taaacacaat ttgatctgcc atttttacaa atccttaatt tagatttttt
     4681 ataagatcaa gttcttcctt tgcgatatca actaaagcat atcgtttaat gtttgatata
     4741 ttcatttcat caagtatatc aactacattt ttatatacag catcatcagt gggcttgatt
     4801 aatacaacca tatccttaat agtagaattc ttttctaaca agactttcct gataccatcc
     4861 gcagaataat ctgttttatt tggtccgtca attggttcat tagcaagtcc gatgtaccag
     4921 aataatttat tgtctttacc aagtattatg ttaaacgctt ttgaggcttt taatgcattt
     4981 tgatcttctt cttttatttc gccttttggt tttaccggta aattaatttc cattgtttgc
     5041 ggcttactaa aagttgttgt aagcataaaa aaggttacaa gtaaaaatcc aagatctacc
     5101 attggtgtaa ggtcaatccg ggtggacatt ttcttcctgc cttttttctt accttttttt
     5161 gcgccagctt gtttttgttc taattcagcc attgattaaa tccctttcaa tttgtttgga
     5221 tccacttcaa gatcggtgat aaggttgaat ttattaacgt ttttatcttg tagtgtattc
     5281 ataacttttt taacaattgg ccaaggacag ccttgatcac cttttacagt gattctaaca
     5341 cctggatttg ataatcttcc ttgcattatc caatctccca attcgttgtt tattgaatcg
     5401 accggtatac ctggttgaga atacattttt ctatcggctt catccagagc taaataccct
     5461 tttaattgat ttaggggtac tcctatgctt cctgtaagaa caaaagcttt ttgctccgca
     5521 gcatcaaatt gaagttctgg atgcttttca cttattttcg ctaaaagttt ttcacgggtg
     5581 aagtttttat tatctatact gaagaaaact tttccatcaa gagtaatcgt gatatttaat
     5641 atatctgtat caggtaattt aatttcagat attgatgaag gtgtatcaac tataacagaa
     5701 tcatcagact taaattgtgt tgttaacata aaaaatgtca gcagcaggaa agcaacgtcg
     5761 cacattgcag tcatatcaat caaagtactt tttctaggaa ttttaacttt aggcattttg
     5821 ttacaccatt atttacttat taatcattat ttatgatgtg atgaaaaatt ctgaactatt
     5881 gctactccag cttcatcaat gctgtaagtc attttatcaa ttttagttgt aaaataacta
     5941 tagaaaataa ttgctaaagc agatgaccca ataccaaaag ctgtgttaat caatgcttca
     6001 gaaattcctg tcgcaagtgc taccgcatct ggtgcacccg ctgttgccaa agcagagaat
     6061 gctttgatca ttccaagtac tgtcccaagt aaacccataa gcgttgctaa tggtgcaatt
     6121 gtagccagta ttacaagatt ctgttcaaga attggtaact ctaaggaggt ggcttcttca
     6181 atttcctgct taattgaagc tatcttttgt tctttgttca tttcactgga ttcagtaaca
     6241 ctcttgtatc tttctaaacc tgctttaaga acatttgcaa cggatcctct ttgtccatca
     6301 cattccatta ttgctttatc aatatcttga cttgaaagct tttgataaac acgaactaca
     6361 aaggaagcaa tgctcccttt accttttgct tttgtaatag ttataaacct ttcaatagta
     6421 aaagtgataa ccatcattaa aattgtcatt aataaaggga caattattcc acctttgtaa
     6481 ataaccccaa gataatttcc aggtttagga agattattaa cgttgtttcc ttgaaaattt
     6541 tcaggattac ccattatgaa gaaataaagt acaagagcaa caacaaataa tactgggatt
     6601 gctattgctg cgaaccctgc gaatgccgat ttaagttcaa atttggtttt caattttttt
     6661 ctcctgacat ataatatttt atgattatta attattagag ctaacagata ttttataatg
     6721 caaataaata attcataatc cttgtagagc tttaagcaga aagctcaatc tggattgatt
     6781 gataaaattt ttgagagtac aagtcagtac agaatctaat tagatccttt tactgagaat
     6841 tattttattt gtttttaaga aaagaatatg gggggacttc gaaggtcaag atgattggtt
     6901 gtaaatttta atatatttat ttcattgaga atttttattg tatataataa ttttatattg
     6961 gaatttcttg caacatcttc cgaatataga atcttctcct ggcctataat ctttagtaag
     7021 gatgtttcac tgcctgatga acgatcaaac cctttcacat tgttctctgt tttacaagga
     7081 gaactctgta tctgatttga tacaactaat atagtttcgt tgttgtttgg attcttctga
     7141 tcatttgagg taaattgtaa acgtgaactg attagctgaa gaagagtgac aatgattaat
     7201 acaattgccg aagaccttat tatatattta ttaaacgttg ataacatttt tttttctctg
     7261 attaaatata aatcaaaata atcaaaaaac aactgaccaa atttcaggtt tattttccca
     7321 tttaatctga taatagcatg ttcaatgtgc attttctgga tgccacgttc aacccaattc
     7381 gcagacggaa ttgatctatt cacttgggca tgagaaccag cagatccaaa tattgtcttt
     7441 cctgatgaac ctgatttaag cagatttgtt tggtctattg acccaagttt ttcaggtgtt
     7501 tctaaaatgc ctcccgtagg atacgcaaga ccagtcactc ataatcaatt atgatgaatt
     7561 aaagcctgaa ttgcagaagg agcattagat aactaaccag aaaataatat taaccgcatt
     7621 acccgccata gttatgacta atgtgaccaa tatttttcca atgaacaggt cgcaggtttt
     7681 caatttcatt cgttcctcct ttttcaattg gaacgatata atctattatc cacccattct
     7741 cagtttcgat taaataatca tcaaagaata ttatagaacc gaaacaatcc tttttataaa
     7801 gttcaaatcg tttatatgga atagctttac gccagacttc tataattgta aagtcatcat
     7861 attcttttgg atttttaaaa ttatttacca tccggatcgc atttttttat tttgacaatc
     7921 taaaattatc taagctggat tttctaccga gcaatgtaat tactacatct ggtttaaatt
     7981 tgtagtcagg ttccggcata agaatatatt tatcatttaa aatttcgttt acaccgataa
     8041 ccagtacatc atattttgat ctaagtttta attcttttaa tgtttttcct gcaaagttat
     8101 cagg
//
LOCUS       DASWBY010000056         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_566384, whole genome shotgun sequence.
ACCESSION   DASWBY010000056 DASWBY010000000
VERSION     DASWBY010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566384"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VF870_01200"
     CDS             complement(<1..1125)
                     /locus_tag="VF870_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX9250822.1"
                     /translation="MKYFIFPFLLFIGLLSINMNAQTMNNDNPFFAEYTTPFQTPPFD
                     QIKPEHYLPAFNEGIKIKRAELDAIINNPDKPTFENTIAAIEKSGELLTKVSRVFFNI
                     SGSNGNDETQKIAETITPDLSKLSNDIYLNENLFQRIKSINDEKEKLNLTAEQLKVLD
                     NYYTDFVRNGIGLDEEKKTRLKAINEELSMLSLKFGDNLRKETNALGLVIDQKEDLVG
                     LPDAVIQGAAELAKQNGLEGKWAFNLQRPSFTPFLQYSEKRDLREKLYKAYLMRGNNN
                     NEYDNKEIIKKMIALRNEKVNLLGYKTYADFKLEKNMAKNPATVNKFLADLWTPALKK
                     SEQEASDMQKIIDADGGNFKLAGWDWWYYAEKVKKEKYALD"
     gene            complement(1179..2702)
                     /locus_tag="VF870_01205"
     CDS             complement(1179..2702)
                     /locus_tag="VF870_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9250823.1"
                     /translation="MSEHKITRNPWAWIPTLYFTQGIPYVMVMSVSVMMYKNLGVSNT
                     DIAFFTSLLYFPWFLKFAWGPFIDMFKTKRFWTVTMQFIVAVGLLGIAFSLHTAIYWQ
                     LTLIVFALMAFASATHDIAADGFYMLSLDQSQQAAFVGVRSTFYRIATIVGSGVLVVI
                     AGQLSSSLGFKNAWSVVFMITAGFFVMLFLYHKIILPYPKEDKGTLLGKKLSGTQIFL
                     LAGGFILFAGVVYLSFLILGFILSLFKIQSPWDTIVSTILLVVIFVILFRTYVATFVD
                     KFEKSEIKNETLLPFIEFLRAFVIFMQKKDIWNILGFLLFFRFAEAQLVKLVQPFLLD
                     PIDKGGLGLTTSEVGIVYGTVGIIALTAGGLIGGYVISRQGLKWWLWPMVIIMHTPDL
                     AFVYLSHVQPTNFVLINLAVAAEQFGYGFGFTAYMMFMIMVSQGEHKTAHYAICTGIM
                     ALGMMLPGMFSGALQEAIGYPGFFEWVLISTIPGFIVAALVKIDPEFGKKKEEPAKV"
     gene            complement(2830..3744)
                     /locus_tag="VF870_01210"
     CDS             complement(2830..3744)
                     /locus_tag="VF870_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX9250824.1"
                     /translation="MKYLIGMDGGGTKTKCVLTDFNLNKIYETAGSASNFLVIGTETV
                     SETILTLINDCASSQKISIGDIEAIVLGTTGGGRRNDAELLEKQIFADAKQKSIPINK
                     FRVESDARIALEGAFSGKEGSILIAGTGSIMFGKDEAGVIHRVGGFGRFIGDEGSGYR
                     IGRIGLNAVARYYDGRAKSTMIADLLEQEFSISSSEELITEVYRNNFNIASAAPLVFE
                     AAESGDKTAQRILEDEATELLLHVSAMKTKLNVEILKVSLIGSILTNPNYFSYLFNEM
                     VVRRFNDVKIMEAEHSPEYGAALMAKQL"
BASE COUNT         1237 a          772 c          616 g         1249 t
ORIGIN      
        1 atcaagtgca tatttttctt tttttacttt ctcagcgtaa taccaccagt cccagccagc
       61 caatttaaag ttgccgccgt ctgcatcgat tattttttgc atgtcagaag cttcctgctc
      121 tgattttttt aatgctggtg tccagagatc cgctaaaaat ttattaactg ttgcgggatt
      181 cttagccata ttcttttcaa gtttaaaatc tgcataagtt ttatatccaa gcagatttac
      241 tttttcatta cgcagcgcaa tcatcttttt aattatttct ttgttgtcat attcattatt
      301 gttattgccg cgcatcaaat aagctttata aagtttttct ctcagatctc ttttttcaga
      361 gtattgcaga aacggagtaa aacttggtcg ttgaagattg aaggcccact tcccttctaa
      421 tccattttgc tttgcaagtt ctgctgcacc ctgaattaca gcatcaggta gtccaaccaa
      481 atcttctttt tgatcaataa ctaatcccag tgcattggtt tcttttcgga gattatcacc
      541 gaactttaag cttagcatac taagctcttc attaatcgct tttaatctgg ttttcttttc
      601 ttcatccagt ccgattccat tacgaacaaa atcagtataa taattatcaa gcacttttag
      661 ctgttcagct gtaagattta atttttcctt ttcatcgttg attgatttta tccgttgaaa
      721 taagttttca ttcaaataaa tatcattgct tagctttgaa agatccggtg taattgtttc
      781 agcaatcttt tgtgtctcat catttccatt agatccgctg atgttaaaaa atacacgtga
      841 tacttttgtg agaagttcac cgctcttttc aattgctgca atcgtatttt caaaagttgg
      901 tttatccgga ttgttgatga ttgcatcaag ttcagcacgt ttgattttta ttccttcatt
      961 aaatgcaggc aggtaatgtt caggttttat ctgatcgaac ggcggagttt gaaatggagt
     1021 tgtatattct gcaaagaatg gattatcatt attcattgtt tgcgcattca tattaattga
     1081 taacaatcca ataaataata gaaatggaaa gatgaagtat ttcatttggt tttctccctt
     1141 gattagaact aatttttatc ataaacgaaa ttaaaaattt aaactttggc tggttcctct
     1201 tttttcttac caaattcagg atcaatctta actaaagcgg caacaataaa tccaggaata
     1261 gttgatatca aaacccattc aaaaaatccc gggtaaccaa tagcttcctg caaagcacct
     1321 gaaaacattc ccggcagcat catacccagg gccataattc cggtacaaat tgcatagtgc
     1381 gcagttttat gttcaccttg cgataccata atcataaaca tcatataagc agtaaaacca
     1441 aatccatatc cgaattgctc tgcagctact gcaagattaa tcaatacaaa atttgtcggt
     1501 tgaacatgcg ataaataaac aaacgcaaga tcaggagtgt gcataattat taccatcggc
     1561 cataaccacc attttaatcc ctgtctggaa attacgtagc cgccaattaa gccgcctgct
     1621 gttaatgcaa taataccaac tgtaccataa acaataccca cttctgaagt tgtaagtcca
     1681 agaccgcctt tatcaattgg atcaagcaaa aacggctgca caagtttaac aagttgtgct
     1741 tctgcaaacc ggaagaaaag aagaaaccca agaatattcc agatatcttt tttctgcata
     1801 aatattacga aggctcttaa aaattctata aatggaagaa gggtttcgtt tttaatctct
     1861 gatttttcaa acttatctac aaaagttgca acataagttc taaaaagaat tacaaatatt
     1921 accaccaaaa gaatagttga aacgattgta tcccacggcg attggatttt aaataaggaa
     1981 agaataaaac caagaattaa aaatgagaga taaacaactc ccgcaaataa aataaatccg
     2041 cctgcaagca aaaagatttg agtcccgctt aattttttac caagtaatgt tcctttatct
     2101 tctttaggat aaggaagaat aattttatga taaagaaaaa gcattacaaa aaaacctgct
     2161 gtaatcataa acacaacaga ccaggcattt ttaaaaccta aacttgagga taattgccct
     2221 gctatcacaa caagtacacc tgaacctaca attgttgcta tgcgataaaa tgttgagcgt
     2281 acacccacaa aagctgcttg ctgggattga tcaagactaa gcatgtaaaa tccatcagca
     2341 gcaatatcgt gtgtagcgga tgcgaatgcc atcagagcaa agactattaa agttaactgc
     2401 cagtaaattg ctgtgtgcag actaaacgca attcctaaaa gaccaacagc gacaataaat
     2461 tgcatcgtaa ctgtccagaa tcttttagtc ttgaacatat caataaatgg tccccacgca
     2521 aactttagaa accacgggaa gtagagcaga cttgtaaaaa atgcaatatc agtgttggat
     2581 acaccaagat ttttatacat cattactgat acagacatta ccattacata agggattcct
     2641 tgtgtaaaat acaaggttgg aatccaggcc catggatttc ttgtaatttt atgttcagac
     2701 attaaagctc cattttttcc agtcattgcg aacgagaagt atgtcggaag actggtcttc
     2761 gactgaagca atctatcaaa ctttagattg attcgtccca acaggtcggg actcgcaatg
     2821 acattctgtt tataattgtt tagccattaa agcagcacca tactcaggag aatgttctgc
     2881 ttccataatt tttacatcat taaatcttct tacaaccatt tcgttaaata agtaagaaaa
     2941 ataatttgga tttgtaagta tgctgccgat taaagatact tttaaaattt ccacatttag
     3001 ttttgttttc atagctgata cgtgaagcaa tagttcagtt gcctcatcct ccagaattct
     3061 ttgagcagtt ttatctccgc tttcggctgc ttcaaataca agcggagcag cagaagcaat
     3121 attaaaatta tttctgtaaa cttctgttat taattcttcg gatgacgaaa tagaaaattc
     3181 ctgttcaagt aaatcagcaa tcatcgttga tttagctctg ccgtcgtaat atcgtgcaac
     3241 agcatttaat ccgattctac cgattctgta accgcttcct tcatcaccaa taaatcgccc
     3301 gaaaccacca acacgatgaa taacgcctgc ttcatcttta ccaaacataa ttgaaccggt
     3361 gcctgcaatt aaaatacttc cctctttacc ggaaaaagcg ccctccaatg caatgcgcgc
     3421 atcgctttca acccggaatt tattaatagg aatagatttt tgtttagcat cggcaaagat
     3481 ttgtttttct aacagttcag cgtcattacg ccgaccgcct ccggtggtcc caagaacaat
     3541 tgcttcaata tctcctatcg aaattttttg agaagaagcg cagtcattta taagagtaag
     3601 gatagtttca gaaacagttt cagttccgat tactaaaaag tttgatgcgg atccagcagt
     3661 ttcatagatc ttatttaaat tgaaatcggt aagaacacat tttgtttttg ttcccccgcc
     3721 gtccattcct attaaatatt tcactgtggt cacctgataa tttttataaa attcttctgc
     3781 aagataaagt ttttaattcc tttaataaac ttattgacca actaaacatt ctatggtttg
     3841 catttaaatc gccaagattc cgggtcaggc tcgg
//
LOCUS       DASWBY010000057        10307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_302978, whole genome shotgun sequence.
ACCESSION   DASWBY010000057 DASWBY010000000
VERSION     DASWBY010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 10307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302978"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VF870_01215"
     CDS             complement(<1..326)
                     /locus_tag="VF870_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9250825.1"
                     /translation="MSQSEKRSVLIIDDDKTIRKLINHHLQLNNFIPFEAENADQAFD
                     ILKKEKIDLVLSDVTMDGLDGFEFCKKVRENENHRFLPFIFVTAKNTLEDKSKAVESG
                     GDDIIT"
     gene            complement(406..744)
                     /locus_tag="VF870_01220"
     CDS             complement(406..744)
                     /locus_tag="VF870_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9250826.1"
                     /translation="MEFNREEKGDVVVITVNLKRATMVEAEEFKQVLVSDIQRDFKKI
                     VVDLSSCEFIDSTFLGSLVVSLKKLTGLGGDLRLVGFQPAVHSMFELTRMYRVFESFK
                     SAEEAVQSFN"
     gene            complement(758..2140)
                     /locus_tag="VF870_01225"
     CDS             complement(758..2140)
                     /locus_tag="VF870_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9250827.1"
                     /translation="MEKLIFIVDDEQAISKLLSYWAKDKWKYQVESFANAEDALKNLN
                     KKPDVILLDIMLPGMDGIETLKRIKQFDEHIPVIMLSAQGSIEVAVEALRYGAFDYFT
                     KPIDQQKLELAIKNSIKSYDLTRELQSLKDNVKKEYSFDNIISSDGKMQDVFKLVTKV
                     LHNDITVLIYGESGTGKELIARAIHYNGQRKDKPFVVVNCASIPRELLESELFGHEKG
                     SFTGAHARKLGKFEIAKGGTIFLDEVGELEMLLQAKLLRVIQQKEFERVGGSELIKTD
                     VRIISATNRDLKKAVENKEFREDLYYRLNSFPISIPPLRHRKSDILILSEHFIQKFSA
                     KLQKSTKGFSKRALKLIYEYNWPGNVREIENTIERCLIITDKDIIDVEDLPAHLRTGE
                     SSNGVEYTGPLFSDETVIPFEKLKEEAIRHALSITNGNIVEAAKRLQLGRATIYRLMD
                     KYKIENRSSE"
     gene            complement(2159..3388)
                     /locus_tag="VF870_01230"
     CDS             complement(2159..3388)
                     /locus_tag="VF870_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9250828.1"
                     /translation="MEEIIEETSNSGSIENIDLEKLLKLGDWKELFTGLFNVARELLF
                     VLDENGSIISINQFGASSLDFSVEELIGKHFMDLIDPTHIQLVNASINLALKNDTSFF
                     EASLINKYEKINRYQISIRTVRKDDRIIGLIGVGKDVSLQRKIENELIDLKPKLVEAE
                     RLIALERARSSHQKMMLDELNKMKSEFVSYISHELRTPLASIVGFSETIASDPNMPNE
                     MRNEFNLIILNEGKRLAKLINDVLDLSRMETGRVAINKSKVNIVKLIHRTIENNRNFI
                     DSKNLILTLEVPNEEIIIEVDEERLLQALDSLMDNAIKFTKEGGRIKVMLNNLYREVE
                     IIVSDTGIGIPVKDLPYLFQKFYRVNRPESDIPGAGMGLVFVKQIVDLHKGLINIQSE
                     LNKGTSVLIKLLKSNRD"
     gene            complement(3501..6884)
                     /locus_tag="VF870_01235"
     CDS             complement(3501..6884)
                     /locus_tag="VF870_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEX9250829.1"
                     /translation="MNTSFYKEIISKLELLIKKEYFSLFLLGLFTALIIIISAFTLFS
                     LFEFVTYSKVIIRTILFFIFLLIALGSISYLIIVPLLKYFNLFGIRDYFYSANKVGKY
                     FPEIKDDLLNAMQLVSSDKTKTIYSIELLDAAFKNVYERSKPIKFESIADFNKVKIIA
                     KYFVLIIVFCALVFALVPGLQAASNRLLSFNQEFIPPAKFYFEVYPGNSEVTKGENVD
                     FVIRVKGEIPQKVFLLTRELSQTNFEENELKLDSLGNYRFTIQQLRSSLLYFAEAENV
                     QSEEFEIKVIDYPVLRSFDVKVLSPGYSGIPIAEQKDNGNISALVGSNVEISLLSNKE
                     LKSARIEFEDSTSQDLQVQNNFAKGSFKVRKDNSYTVLINDLSGNKNLQPIKYYVKAL
                     YDSNPTIELIYPKQDINLANDSRVPIEIKVNDDYGFSKLNLNYKLSSSRYEPAHDNFS
                     IIELAVDKKIKEQIIDYIWNLTKLNPAVNDIYSFYLEIFDNDNISGPKSAKSQIINVR
                     VPSLDEILAKADDTQENVQDEMQETLKQAEELKKELEKIDKDLKQDKQQLTWEEKEKI
                     EQTLDKFEKLQEKMESASEQLKQMQNELQQKQLLSEETLEKYMELQKLMDEMTSEEMK
                     KAMKQMQDALEKMNRNQTQDAMTQMKMDEEKFKKSIERTMNLLKRIQIEQKMDEMIKR
                     TENIEQKQNELNEQTQKSDQSDQKQSDELSKKQDEVTKELDRLQEKMDELAEKMKELK
                     DMPNEEMEKLQEEMDKQKNEEMSEEASKDLKQNQKQEAQKNQQKLSKNMQQMKSSMQQ
                     MQEQMQQENQMQTFTDMMRILDNILSLSKQQEDLKNKLQNVDPSSAQFEQNLQKQNEI
                     KKNLNKLLEQMSELSQKTFAISPEMGKALGNANQKMDQSMQSMQNRNGSMATLSQGDA
                     MMHLNEAANMMKSSLESMMQGGSGNGGMMSLMQQLQKMSGQQMDLNNMTQMLQQMQQG
                     QLSPEQQGQMQRLAQQQQMIQKSVEQLNKEAKQSGVSKKIPADLDNIVNQMREVVSDM
                     NTEKLSDDLIQKQERILSKLLDAQKSINERDFEKERKSESGNNFTRNSPGELNLRNQQ
                     GKEKLKDELNRAVQEGYNKDYEELIKKYYEALQQERIKN"
     gene            complement(6893..7558)
                     /locus_tag="VF870_01240"
     CDS             complement(6893..7558)
                     /locus_tag="VF870_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX9250830.1"
                     /translation="MIIKKTIFLLIILFYSVCSYSQNETGFQIARLKYSGGGDWYNDP
                     SAEVNLLKFVQANTNIKVNAEYKFVDIASDEIFSYPFLFLTGHGNIVLSTEEAKRLRT
                     YLGNGGFLYIDDDYGLDKAVRREMKKVFPENDFIEVPYSHKIFNIFYKFENGTPKTHE
                     HDKKPPQSFGIFLGTRLAVLYTYESNPSDGWADPEVHNDPKDKREEALKFGTNIIIYA
                     LSK"
     gene            7644..8108
                     /locus_tag="VF870_01245"
     CDS             7644..8108
                     /locus_tag="VF870_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX9250831.1"
                     /translation="MKKYWLVKSEPETFSLDDLQKSKNQTTCWDGVRNYQARNYLRDE
                     MKKGDQVLFYHSGAENLAVMGICEVVKEGYPDHTQFDPDNDHYDPKADPKNPTWIMVD
                     VKFVKEFKTPVTLDAIKSNLKLKNMKLIARGNRLSVMPVTKDEFDEINKMGR"
     gene            8114..8186
                     /locus_tag="VF870_01250"
     tRNA            8114..8186
                     /locus_tag="VF870_01250"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8147..8149,aa:Ala,seq:cgc)
     gene            complement(8194..8625)
                     /locus_tag="VF870_01255"
     CDS             complement(8194..8625)
                     /locus_tag="VF870_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9250832.1"
                     /translation="MIVRPPKTPEEFERYRDLRWRILRAPWNQPRGSEVDELESKAFP
                     IMVYEVDGIPVGVGRVHFNSETEAQIRSMAVEEEWRGKGIGSIVVKELEKIAIEKGAK
                     KIILHARDNAVKFYERNGYKVVKQSHTLFGVIPHFLMEKEV"
     gene            complement(8622..9980)
                     /gene="rmuC"
                     /locus_tag="VF870_01260"
     CDS             complement(8622..9980)
                     /gene="rmuC"
                     /locus_tag="VF870_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX9250833.1"
                     /translation="MEIIYLIIGLIIGLVIAFFFFKSKKTVSIDEVNKLNEQINSSKV
                     DVGKLTERTKLLEVDKVNLQTEIRTEREKSEKLTSENSSLKSDYANLQEKLSEQKSEV
                     EKLQEKFTKEFQNLANKILDDNSKKFSEQNKEKLSEILNPLKEKISDFEKKVEETNKE
                     SIKGHASLKEQLQMLKEMNQQITQEAKNLTTALKGQSKTQGNWGEFILESILEKSGLV
                     KGREYVVQESLTAESGKRYQPDVIINLPENKSIVIDSKVSLVAYERFFSTEEKDEKER
                     ALKEHIISIRAHVKNLSSKGYQDLYQLHTLDFVIMFIPIESALALAVQNDPNLSTEAF
                     EQRILILSPSILLAALKIFSSIWQQEKQNQNALDIAKQSGALYDKFVGFIEDLKLVGD
                     RMDQAKTSYVNAMSKLVDGSGNLVGRVEKIKKLGAKANKSLPPNILNRADIDDDNLLE
                     NE"
     gene            complement(10099..>10307)
                     /locus_tag="VF870_01265"
     CDS             complement(10099..>10307)
                     /locus_tag="VF870_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine ammonia-lyase"
                     /protein_id="HEX9250834.1"
                     /translation="EFMAAAQALDFRENVHGKGVAKSKEVIRKHVEFLDIDRPLYADH
                     NKMKTLVKSCEILEEVEKEIGSLE"
BASE COUNT         3016 a         1945 c         1509 g         3837 t
ORIGIN      
        1 tttgtaatta tatcatcacc gccggactct acagcttttg acttatcttc aagagtattt
       61 tttgcagtta caaatatgaa aggaagaaat ctatgatttt cattttcacg aactttttta
      121 caaaactcaa atccatccaa accatccata gtaacatctg aaaggacaag atcgattttt
      181 tctttcttta aaatatcaaa tgcctggtcc gcattttcag cttcaaacgg gatgaagtta
      241 ttcaattgca ggtgatgatt aataagtttg cgaattgtct tatcatcatc aattattaga
      301 acagatcttt tttcagattg ggacatttat aagcagctta atatataatt atagattatg
      361 aatcctttta tcatttccgg aaaagcgcct taagaaacaa aaatgttaat taaaactttg
      421 gacagcttct tctgcagatt taaaggactc aaacacacga tacattcttg ttaattcaaa
      481 cattgaatga actgcgggtt gaaaaccaac caagcgaaga tcgccgccaa gtcctgttaa
      541 tttttttaat gaaacaacaa gacttccaag aaaagttgaa tcgatgaatt cacaactgga
      601 aagatcaaca acaatttttt taaagtcccg ttgtatatcg ctaaccaaaa cttgcttaaa
      661 ctcttcagct tctaccattg tagcacgttt taaattaaca gtaattacta caacgtcacc
      721 cttttcttct cgattaaatt ccatattatt tttacctcta ttccgatgat cggttttcga
      781 ttttgtattt atccattagc ctgtagattg ttgctctgcc aagctgtaat ctttttgctg
      841 cctctacaat attaccatta gtaatactaa gtgcatgtct aattgcttct tcttttaatt
      901 tttcaaacgg aattactgtt tcatcactaa ataatgggcc agtatattca actccatttg
      961 aactttcgcc ggttcttaaa tgtgccggaa gatcttcaac atcaattata tctttatcag
     1021 tgataatcaa acatctttca atagtatttt caatctcgcg aacatttcca ggccaattat
     1081 attcatatat aagttttaaa gcccgtttag aaaaaccttt agtgcttttt tgaagcttag
     1141 cgctgaactt ctgaataaaa tgttccgaaa gaattaatat atcgctttta cgatgtctta
     1201 aaggtggtat tgaaattggg aaagagttca atctataata taaatcttct ctaaattctt
     1261 tattctctac agcttttttc aaatccctat tagttgctga tatgattcta acatcagttt
     1321 taattagttc tgatccgcct actctttcaa attctttttg ctgaattaca cgaagaagtt
     1381 ttgcctgaag cagcatctct aattcaccga cttcatccag gaatattgtt ccgccttttg
     1441 ctatttcaaa cttaccaagc tttcgtgcgt gcgcgcctgt aaaagatcct ttttcgtggc
     1501 cgaacaattc actttcaaga agttcacgtg gtattgatgc acaattaacc acaacaaaag
     1561 gtttatcttt tctttgtccg ttataatgga ttgcacgcgc aattagttct tttcctgttc
     1621 cactttcgcc atatattaat acggtaatat cattatgcaa aacttttgta accagcttaa
     1681 atacatcctg cattttccca tcggaggaaa taatattatc aaaactgtat tcttttttaa
     1741 cattatcttt taagctttga agctcacgtg taaggtcata actctttata gagtttttga
     1801 tggcaagctc taacttctgc tggtcaatcg gtttggtaaa ataatcgaaa gctccatatc
     1861 taagagcttc aaccgcaact tcaatgctgc cctgtgcaga aagcataatt accggaatat
     1921 gttcatcgaa ctgtttgatt cttttcagag tttcaatacc atccatcccc ggcagcataa
     1981 tgtctaaaag aattacatct ggttttttgt taagattttt taacgcgtct tctgcattag
     2041 cgaacgattc tacctgatac ttccatttat ctttagccca atagctaaga agttttgaaa
     2101 ttgcttgttc atcatctact ataaagatca gtttttccac ttttctatcc tattgtttct
     2161 aatctcggtt actctttaat aatttaatta aaacagaggt gcctttattt agttcgctct
     2221 gaatattgat taaacctttg tgcaaatcta caatttgttt tacaaaaacc aatcccatgc
     2281 ccgccccggg aatgtcagat tctggcctat ttaccctgta aaatttctga aacagatacg
     2341 gtaaatcctt tacaggaatg ccgattccgg tatcagaaac tataatttca acttcacgat
     2401 aaagattatt tagcattacc tttattcgtc cgccttcttt agtaaacttt attgcattat
     2461 ccatcaaaga gtctaaagcc tgcagcaatc gttcttcatc aacctcaatt ataatttctt
     2521 cattaggcac ttctaaagta aggatcaaat ttttggaatc aataaaattc ctgttattct
     2581 caatagttct gtggattaat ttaacaatat taacttttga tttatttata gccactctgc
     2641 ctgtttccat tcgggaaaga tcaagaacat catttataag tttagctaat cttttacctt
     2701 cgttaagaat tattagatta aactcgtttc tcatttcatt cggcatattt ggatccgaag
     2761 ctattgtttc ggaaaaacca actatcgatg caagcggagt gcgtaactcg tgagaaatat
     2821 aagaaacaaa ttcacttttc attttgttta gttcatccag catcattttt tgatgagatg
     2881 atcttgctct ttctaaagcg attaatcttt cagcctctac taattttggt ttaaggtcaa
     2941 ttaactcatt ttcaattttt cgctgcaacg atacatcttt tccaacacca atcaacccta
     3001 ttatccgatc atctttcctg acggttctta tgctgatttg atagcggttt atcttttcat
     3061 atttattaat tagagatgct tcaaaaaatg aggtatcgtt ttttagagca agatttatgg
     3121 atgcatttac aagctggatg tgtgtgggat ctattaaatc cataaaatgc ttgccaatta
     3181 attcttcgac agaaaaatct aaggaagatg cgccaaactg atttatggaa attattgaac
     3241 cgttttcatc aagaacaaaa agcaattccc ttgcaacatt aaaaagtcct gtaaaaagtt
     3301 ctttccaatc tccaagcttt agtaattttt caagatcaat attttcgatt gatcccgaat
     3361 ttgatgtttc ttctattatc tcttccaaca tcttatgaaa tgaattacgt ttaaaatcat
     3421 gatgaataat ggcatagaaa atagcatgat tttgattgga aatctatcat tacggaaaca
     3481 ataaaatatc aatagtaagt ctaattctta attctttcct gttgcaaagc ttcataatat
     3541 tttttaatca attcctcgta atccttattg tacccttcct gaacagccct attcaattca
     3601 tctttcagtt tttctttgcc ctgctgattc cttagattta attctccagg actattacga
     3661 gtaaaattat ttccactttc agactttctt tctttttcaa aatctctttc gtttatagat
     3721 ttctgagcat ccaggagttt tgataaaatt ctttcctgct tctgaattag atcatcactt
     3781 aatttttcag tattcatatc gctgacaact tctctcatct ggttgacaat attatcgaga
     3841 tctgcaggaa tttttttaga cacgccgctt tgcttggctt ctttgttcaa ctgctcaaca
     3901 gatttttgaa tcatttgctg ctgctgtgct aatctttgca tctgtccttg ctgctcgggt
     3961 gataattgcc cctgctgcat ttgctgaagc atttgagtca tattgttcaa atccatttgc
     4021 tggccagaca ttttttgcaa ctgctgcatc aaagacatca ttccaccatt tccgctgccg
     4081 ccttgcatca tcgattccag tgaactcttc atcatatttg cagcctcatt caaatgcatc
     4141 atcgcatcac cttgcgaaag agttgccata cttccatttc tgttttgcat cgactgcatt
     4201 gattgatcca ttttctgatt tgcattgcca agtgctttgc ccatttctgg tgagattgca
     4261 aatgtctttt gtgatagttc agacatttgt tccaaaagtt tatttagatt ttttttgatc
     4321 tcattctgct tttgtaaatt ctgttcaaac tgagctgaac ttggatcaac attttgaagc
     4381 ttatttttaa gatcttcctg ctgttttgat aacgataaaa tattatcaag aatgcgcatc
     4441 atatcagtaa aagtctgcat ttgattttcc tgctgcatct gctcctgcat ttgctgcatt
     4501 gatgatttca tctgctgcat gtttttagaa agcttctgct ggtttttttg agcttcctgt
     4561 ttttgatttt gctttaaatc tttagatgct tcctcagaca tttcttcgtt tttctgttta
     4621 tccatttctt cctgcagttt ttccatttcc tcatttggca tatccttcaa ctctttcatc
     4681 ttttctgcaa gctcatccat tttttcctgt aagcgatcaa gctctttagt aacttcatct
     4741 tgttttttac tcagttcgtc agattgcttc tgatctgatt gatcgctttt ttgtgtttgt
     4801 tcattaagtt cattctgctt ttgttcaata ttttcagttc gtttaatcat ctcatccatt
     4861 ttctgttcaa tctgaattct ctttaacaaa ttcatcgttc gttcgatact ctttttaaac
     4921 ttttcctcat ccatcttcat ttgggtcatt gcatcctgag tttgattacg gttcattttc
     4981 tcaagagcat cctgcatttg cttcatcgct tttttcatct cttcacttgt catctcatcc
     5041 atcaacttct gcaactccat atacttctca agagtttcct cagaaagcaa ttgtttctgc
     5101 tgaagttcat tctgcatttg ttttaattgc tcgcttgcgc tctccatttt ttcctggagt
     5161 ttttcaaatt tatccagcgt ttgttctatc ttttcttttt cctcccacgt taattgctgt
     5221 ttatcctgtt ttaaatcttt atcaatcttt tctaattctt tttttaattc ctcggcttgt
     5281 tttaatgtct cctgcatctc atcctgaaca ttttcctgcg tatcatcggc ttttgcaagt
     5341 atttcatcaa gtgaaggaac gcgaacatta attatttgtg actttgcaga ttttggccca
     5401 cttatattat cgttatcaaa tatttcaaga tagaatgagt aaatatcatt aactgcgggg
     5461 tttagttttg ttagattcca gatataatca atgatctgct cttttatttt tttatctacg
     5521 gctaattcta ttattgaaaa attatcgtga gctggttcat atcgagagga gcttaattta
     5581 taattaagat tcagttttga aaaaccatag tcatcattta ccttaatttc tataggaact
     5641 ctgctgtcat tggcaagatt tatatcctgt ttgggataaa tcaattcaat tgtgggattc
     5701 gaatcataca gtgctttaac atagtatttt attggctgaa gatttttatt gccggataga
     5761 tcattaatta aaacggtgta ggaattatct tttctaactt taaatgatcc ttttgcaaaa
     5821 ttattttgaa cttgaagatc ctgagaagtt gaatcctcga attctattct tgctgatttt
     5881 aattctttat ttgaaagcag gctgatctca acattacttc caactaatgc agaaatgtta
     5941 ccgttatctt tttgttcagc tattggaatg cccgaataac ccggcgataa aacttttaca
     6001 tcaaaagaac gtaacaccgg ataatcaatt acttttattt caaattcttc gctttgtaca
     6061 ttttcagctt cagcaaagta cagaagtgaa gatcgaagtt gttgtattgt aaatctataa
     6121 ttcccaagtg aatctaattt caattcattt tcttcaaaat tagtttgaga taattcccgt
     6181 gttaataaaa atactttttg gggaatttct ccttttaccc gaattacaaa atcgacattt
     6241 tctccctttg ttacttcaga atttccagga taaacttcaa aataaaattt tgcgggcgga
     6301 ataaattcct gattgaaact taataatcta tttgaggccg cctgcaatcc gggaaccaac
     6361 gcaaacacca aagcacagaa gacaataatt aagacaaaat actttgcaat aatttttact
     6421 ttattaaaat cagctatgga ttcaaactta attggcttag atcgttcata aacattttta
     6481 aaagcagcat ctaacagttc tattgagtat atagtttttg ttttatcaga tgaaacaagc
     6541 tgcattgcgt taagaagatc atctttgatt tccgggaaat actttccaac tttatttgca
     6601 gagtaaaagt agtctcttat tccaaataaa ttaaaatact ttaataatgg aacgatgatg
     6661 agataactaa ttgaaccaag tgcaatcaaa agaaaaataa agaaaagaat tgttctgata
     6721 atcactttgg aataagtaac gaactcaaag agactaaata aagtaaatgc actgataatt
     6781 attattaatg cggtaaataa tcccagcagg aaaagtgaga agtactcttt tttaataagt
     6841 agttctaatt tgcttattat ttctttataa aaacttgtgt tcacttgtta actcatttac
     6901 tcaaagcata aatgattatg ttagttccaa acttaagtgc ttcttctctt ttgtctttag
     6961 ggtcgttatg cacttctgga tcagcccagc catcgcttgg gtttgattca taagtgtaaa
     7021 gtactgctaa tcttgttcct aaaaatattc caaaactctg cggtggtttt ttatcgtgct
     7081 catgtgtttt gggtgtgccg ttttcaaatt tgtaaaagat attaaaaatc ttgtgagaat
     7141 aaggaacttc aataaaatca ttttctggaa atactttttt catttctcgt cttactgctt
     7201 tatccaaacc atagtcatca tctatataca gaaaccctcc atttccaaga taagttctta
     7261 atctttttgc ttcttcggtt gataatacaa tattcccgtg cccggtcaaa aacaaaaaag
     7321 gataggagaa gatctcatct gaagcaatat caacaaattt atattcagca ttaactttaa
     7381 tatttgtgtt agcctgaaca aacttaagca aattaacttc tgcagatgga tcattatacc
     7441 aatctcctcc gccgctgtac ttaagacgcg ctatttggaa tcctgtttca ttctgtgagt
     7501 aactgcatac agaataaaaa agaataatta acagaaatat tgttttctta ataatcattg
     7561 tgatttaata tatttacaca taaagactat agcaatttag aaaattaaca agaataatca
     7621 gtaccaaaat ttggagttta aacatgaaaa aatattggct tgtaaaatca gaaccagaaa
     7681 cattttcctt agatgatctt cagaaaagca aaaaccaaac aacttgttgg gatggagtta
     7741 gaaattacca ggcaagaaat tatttacgcg atgaaatgaa aaaaggtgac caggttttgt
     7801 tctatcatag cggtgcagaa aatcttgcag taatgggaat ttgtgaagtt gtaaaggaag
     7861 gatatccgga tcatacacag tttgatcccg ataacgatca ttatgatcca aaagctgatc
     7921 ctaaaaatcc aacctggatt atggttgatg taaagtttgt taaagaattt aaaactcccg
     7981 ttactcttga tgcaatcaaa tcaaatttaa aactaaagaa tatgaaattg atcgctcgtg
     8041 gtaatcgctt atcagttatg ccggtaacca aagatgagtt tgatgagata aataaaatgg
     8101 gaagataaaa atcggggctg tagctcagtt gggagagcgg ttcgttcgca acgaacaggt
     8161 cgtcggttcg atcccgatca gctccacaaa gaattaaact tctttctcca ttaaaaaatg
     8221 cggaattact ccaaacaaag tgtgcgattg ttttacgact ttgtaaccat ttctttcgta
     8281 aaacttaacc gcattatctc gagcatgcag aattattttc tttgcacctt tttcaattgc
     8341 aattttttct aattccttaa ctacaattga accaattcct ttccctcgcc attcttcttc
     8401 aacagccata gaacggattt gcgcctcagt ttcagaattg aaatgaactc ttccaactcc
     8461 aaccgggatc ccatctactt cataaaccat aattggaaat gctttggatt caagttcatc
     8521 aacttcactt cctcttggct gattccatgg ggcgcgaaga attcgccatc tcaaatctct
     8581 gtaccgttca aattcttcag gagtttttgg cggtctaact atcattcatt ctccaacagg
     8641 ttatcatcat caatatcagc gcgattaaga atattcggtg gtaaagattt gtttgctttt
     8701 gctccaagct ttttaatttt ttcgactctt ccgactaaat ttcctgatcc atcaactaat
     8761 ttactcatcg cattcacata actagtttta gcttgatcca ttctatctcc aactaatttt
     8821 aagtcctcga taaatccaac gaatttatca tataaagcac cagattgttt agcaatatcg
     8881 agcgcatttt gattttgttt ttcttgctgc caaatgcttg aaaatatttt taatgccgct
     8941 aaaagaattg aggggcttag aataaggatc ctttgttcaa atgcttcagt tgataaattt
     9001 gggtcatttt gtacagccaa ggctaaagca gattcaattg gtataaacat aattacaaag
     9061 tctaatgtat gaagctgata tagatcttga tatcctttag agctaaggtt tttgacatgt
     9121 gctctgatag agataatatg ttctttcaat gctctctctt tttcatcctt ttcttcagtg
     9181 gagaaaaatc tttcatatgc aaccaaagat actttcgaat caataactat acttttattt
     9241 tcaggaagat taattatcac atcaggttgg tatcttttgc cggattcagc agttaaactt
     9301 tcctgtacaa catattctct tccttttacc aagccggatt tctcaagaat actttcaaga
     9361 ataaactcac cccaattacc ctgagttttg ctttgtcctt ttaaggcagt tgtcaaattc
     9421 tttgcttcct gagttatctg ctgattcatt tcctttagca tttgaagctg ttcttttaac
     9481 gatgcgtgtc ctttgatgct ttctttgttt gtctcttcaa ccttcttttc aaaatcagaa
     9541 attttttctt ttaatggatt taatatctca ctcaactttt ctttgttctg ctcagaaaat
     9601 tttttactgt tatcatcaag aatcttattt gcgagatttt gaaattcttt tgtgaatttt
     9661 tcctggagtt tttctacttc ggatttttgt tcagataatt tttcttgaag atttgcgtaa
     9721 tcagatttaa gtgatgaatt ttcagaagta agcttttcag atttttctct ttctgttctt
     9781 atttctgtct gaagattaac tttatcaact tctaaaagtt ttgttcgttc ggtaagtttt
     9841 ccgacatcaa cttttgatga gttgatttgc tcgttaagtt tatttacttc atcgattgaa
     9901 actgtttttt tgctcttgaa aaagaaaaaa gcaattacta atccgattat taaaccaatt
     9961 attagataaa ttatttccat tttttgaacc taaatttttt taattacgat agaacacgga
    10021 taatacggat tgctctgatt cccagggatc taatcagcga taacccgtat gatttgtgta
    10081 atccgcgttc aatttttttt attccaaact accaatttct ttttcaactt cctcaagaat
    10141 ttcacaggat tttacaagtg ttttcatctt gttatgatca gcataaagcg gtcgatcaat
    10201 atcaagaaac tcaacgtgtt tacgaattac ttcttttgat tttgcaactc cttttccatg
    10261 aacattttca cgaaaatcaa gagcttgagc agcagccata aattcta
//
LOCUS       DASWBY010000058         3535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_603710, whole genome shotgun sequence.
ACCESSION   DASWBY010000058 DASWBY010000000
VERSION     DASWBY010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603710"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1655
                     /locus_tag="VF870_01270"
     CDS             <1..1655
                     /locus_tag="VF870_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9250835.1"
                     /translation="KRLIEFNAMDFDDLLLKPIELFNEKKHILQKYKSRFEYLLVDEF
                     QDTNKAQYELLKLLVSRNGLISVVGDDAQSIYSWRGANIGNMRDFGKDFPKYKLFKLE
                     QNYRSTKMILKAADSVIKNNKDQVIKTLWTENEEGEPVMLLKCADEKDEASQISKYIK
                     HELTKKKLIFNDFAILYRTNSQSRAMEDIFRREKIPYTIIGGVEFYKRKEVKDVVAYL
                     RVISNQNDEESLLRIMNFPQRGIGSTTVTRMIAFARKHEITLFQTMGRVFEVIDIKER
                     IQKNVKQFKTLLDKYIGLKDKLSVGELTRAMVDDLGLIRMFKEEGTQEALQRMENISQ
                     LLASLSEFSSANSDAKLEQYLEEVSLIADIDNYSESKNCVTLLTVHAAKGLEWPIVFV
                     SGCEEEIFPIASKFLSDASVDEERRLFYVALTRAKKKAYITHARSRYRFGEVAYQSRS
                     RFIDELDPTAYKEVNGGLGRKANRKTKKEMYYEYFEKVEYESFDQDGVELRVGSRVMH
                     DKFGLGKVVDVVGTGEQTKATVHFEGNNVKQLMLKFAKLKVL"
     gene            1707..3074
                     /gene="pfp"
                     /locus_tag="VF870_01275"
     CDS             1707..3074
                     /gene="pfp"
                     /locus_tag="VF870_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEX9250836.1"
                     /translation="MSIAPKKLAILVGGGPAPGINSVIGAATIRAAVEGVDIIGIKDG
                     FKWLMQGDITHVKELNIQNVSRIHFRGGSYIGISRANPTKDPKHLENAVTSLLRLNVD
                     KLITIGGDDTAFSALKVNEMAHGRIKVVHVPKTIDNDLDLPHGIPTFGFQTARHIGVD
                     IVKNLMVDAQTTSRWYFVVSMGRKAGHLALGIGKATGATLTLIPEEFANKQIKFGHIV
                     DILVGAIIKRLSYGKPDGVAIIAEGLVEHLDPADLESLVEVERDAHDNIRIAEINFGE
                     ILKSKVQDRLKEFGLKSTIVAKNIGYELRCADPIPFDMEYTRDLGFSAAQFILDGGSG
                     AMVSIQNAKFVPMYFKDIIDPKTLKTKVRMVDPASESFYIARKYMLRLNQADFDDPHE
                     LAKLAATAGMSIEEFQSKFGYLIENDLLYQFIKDGKFAINSPESNLAHRMQAENEKSE
                     GYDED"
     gene            complement(3242..>3535)
                     /locus_tag="VF870_01280"
     CDS             complement(3242..>3535)
                     /locus_tag="VF870_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HEX9250837.1"
                     /translation="FMWVFGADKAWKEMNQGGDIQIPIIFYYIMKYVTPLILMVLMIW
                     WFIQSAVPTLLLQDAAPENVPYIWGARILMVAIAVILMLLVKKAWENNYSEVK"
BASE COUNT         1236 a          528 c          699 g         1072 t
ORIGIN      
        1 aaaaaagatt aattgaattt aatgcaatgg attttgatga tctgctgctt aaaccaattg
       61 aactgtttaa tgaaaagaaa catattttac agaagtataa atccagattt gaatatctgt
      121 tggtagatga atttcaggat accaacaaag cccagtacga attattaaaa ttactcgttt
      181 caagaaacgg attaatttca gttgtcgggg atgacgctca aagtatttac agctggcgcg
      241 gagctaacat agggaatatg agagattttg gaaaagattt tcctaagtac aaactgttta
      301 aacttgagca gaattacaga tcaacaaaaa tgattttgaa agcagcagat tctgttatta
      361 agaataataa ggatcaggtt atcaaaactc tttggactga aaatgaagag ggcgaacctg
      421 taatgctcct taagtgtgcg gatgaaaagg acgaagcatc gcagatttct aagtatataa
      481 aacatgaact gaccaagaaa aaattaatct ttaacgattt tgcaatttta tatcgtacta
      541 attcccaatc ccgtgcaatg gaagatatct ttcgaagaga aaaaattcca tatacaataa
      601 ttggcggtgt agagttttat aaaagaaaag aagttaaaga cgtagttgct tacttgcgcg
      661 ttatctccaa ccagaacgat gaagaaagtt tgttaagaat aatgaatttt ccacagcgcg
      721 gcattggaag cacaactgtt accagaatga ttgcttttgc acgaaagcat gaaataactc
      781 ttttccaaac tatgggtaga gtttttgaag taattgatat taaagaacga attcaaaaaa
      841 atgttaaaca gtttaaaact ttgcttgata agtatattgg tttaaaggac aaactttctg
      901 taggtgaatt aacccgtgca atggttgatg accttggatt gattagaatg tttaaagaag
      961 agggcacaca agaggcctta cagagaatgg aaaacattag tcagttatta gcctcgttaa
     1021 gtgaattttc ttctgcaaat tcagatgcta aacttgagca gtatctggaa gaggtttctt
     1081 tgattgcgga tattgataat tattcagaat caaaaaattg tgtaacactt ttaactgtac
     1141 atgccgctaa aggattggaa tggccgatag tatttgtaag tggatgcgaa gaagaaatct
     1201 ttcctattgc aagtaagttt ttgtccgatg cttcggtaga tgaagaacga agattatttt
     1261 acgtggctct gaccagggca aagaaaaaag cttatattac tcatgcacgt tcgagataca
     1321 gatttgggga agtagcatat caaagcagat ccagatttat agatgaactt gatccaacgg
     1381 cttataaaga agtgaatggc ggacttggca ggaaagcaaa tcgtaagact aaaaaagaaa
     1441 tgtattacga gtattttgaa aaagtggaat atgaaagctt tgatcaggat ggagttgaac
     1501 tgcgtgtagg cagcagggta atgcacgata aatttggtct tggaaaagta gttgatgtcg
     1561 ttgggacagg agagcagact aaagcaactg tgcactttga gggaaataat gttaaacaat
     1621 tgatgctaaa gtttgctaag cttaaagtac tctaataatt ttcaattaaa gtaatcataa
     1681 ttttttattt aggagttaaa aattaaatgt ctattgcacc caaaaaatta gccatattag
     1741 tcggaggagg acctgcacca ggaattaata gtgtcattgg tgcggctaca attcgtgctg
     1801 ctgttgaagg tgttgatatt attggaataa aagatggttt taagtggtta atgcaagggg
     1861 atattacaca cgttaaagaa ttaaatatcc aaaacgtaag ccgtattcac tttaggggtg
     1921 gttcttatat tggaatatca agagcaaatc ctaccaagga tcctaaacat ttagaaaatg
     1981 ctgttacatc tttattaaga ttaaatgttg acaaactaat aactatcggt ggtgatgata
     2041 ccgcatttag cgctttaaaa gttaatgaga tggcacatgg cagaataaaa gttgtccacg
     2101 ttcctaaaac cattgataat gatcttgatc ttcctcatgg tattcccaca tttggatttc
     2161 aaacagcgag acatattgga gttgatattg taaaaaatct tatggttgat gctcaaacaa
     2221 cttcccgatg gtactttgtg gtttcaatgg gaagaaaagc aggacatctt gcacttggaa
     2281 ttggaaaagc gaccggcgca acacttacac taattccgga agagtttgcc aataaacaaa
     2341 ttaaatttgg tcatattgtt gatatacttg tcggcgctat tattaaaaga ttaagctatg
     2401 gtaaaccgga tggcgttgcg ataattgctg aaggtttagt tgaacattta gatcctgctg
     2461 atcttgagtc tttggttgag gttgagagag atgcacacga taacattagg atcgctgaaa
     2521 taaattttgg tgaaatttta aaatctaaag tacaggatag actaaaagag tttggattaa
     2581 aatcaacaat cgttgcaaag aatattggat acgaactacg ctgcgctgat ccgattcctt
     2641 ttgatatgga atatacaaga gatctaggtt tttcagctgc gcaatttatt cttgatggtg
     2701 gaagcggagc gatggtttcg attcaaaatg caaaatttgt tcctatgtat tttaaagaca
     2761 ttattgatcc taaaacttta aaaactaaag ttagaatggt tgatcctgca tcggaaagtt
     2821 tttacatcgc aagaaaatat atgttaagat taaatcaagc agattttgat gatccgcatg
     2881 aacttgctaa acttgctgct acagcaggaa tgtctattga agagttccag agtaaatttg
     2941 gttatctgat tgaaaacgat ttactatatc agtttattaa ggatggaaaa tttgcaatca
     3001 actcaccgga gtctaatctt gcacatagaa tgcaggcaga gaatgaaaag tctgaagggt
     3061 atgatgaaga ttagtttctt aaactaaaaa tttctcaaaa aaaacccgag ctataaactc
     3121 gggttttttt attaattctt tttctgtgtt ttaaaaactt tatagaatga aattattact
     3181 aatgaaacta taattcccca ggctaatgct aaaaaaatta taccgtttgt tgttaatccc
     3241 attatttaac ctcactgtaa ttattttccc aagccttctt aaccaataac attaaaatta
     3301 ccgcaattgc aaccattaaa attctagcac cccaaatgta tggcacattt tccggagccg
     3361 cgtcttgcaa aagcaatgta ggtactgcac tctgtataaa ccaccagatc attaatacca
     3421 tcaaaataag cggagtaaca tatttcatga tatagtagaa gattatcgga atttgaatat
     3481 ccccaccttg attcatttct ttccatgctt tatcagcacc aaacacccac ataaa
//
LOCUS       DASWBY010000059         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_152691, whole genome shotgun sequence.
ACCESSION   DASWBY010000059 DASWBY010000000
VERSION     DASWBY010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152691"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VF870_01285"
     CDS             <1..1300
                     /locus_tag="VF870_01285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX9250838.1"
                     /translation="IEDRVKTLMSELDKSTSEVEYLNKKLHDVFKDKISELNFEINQR
                     KKAELSLQKSEEQFRLVFQNAPIGIVIISTEGKIISVNKSFCDTIGFERDEIIGIPIK
                     YLFEQNDLEGFSEGTLDNNGNPVADINTEKMLLKKEGKEIHVIVKSVSVLDENGKVKH
                     YVMQLLDISEIKRVQRELVDALNKAEESDRLKSAFLAQMSHEIRTPLNLILTSIPLIA
                     DEISSEDEDLKIILDSVKSAGRRLQRTIDMILSMSSVQSGNYKATFEKFDIVPDLQNM
                     VKEFKSLSDDKGLKLKFSQSDDSAFITADRYTVNQVFQNLINNAIKYTLKGYVEVYVK
                     NLQNDKVKIEIRDSGIGMSEEYLQRMFTPFSQEDTGYKREFEGNGLGLALVKKYIELN
                     RAEISVESEKNIGSVFSVVFDREVTFTTPVESEKRYKSYN"
     gene            1420..>2283
                     /locus_tag="VF870_01290"
     CDS             1420..>2283
                     /locus_tag="VF870_01290"
                     /inference="COORDINATES: protein motif:HMM:NF001135.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEX9250839.1"
                     /translation="MSKYKVISFYKYINIEIPEELAKEHLNWCLENGIKGKVYFAKEG
                     ISGSIFGDNEVTRKYKNHLKSHNIFEDIWFKETPTDRVAFNKMHVRVKNEIVNSGLAG
                     TSLEHTAPRLTPEQLLKFYENKKEFVIVDARNWYESKIGKFKNAITPQITHFREWPKV
                     VESLSEYKDKTIVTYCTGGISCEKASAYMREQGFKDVYQIDGGILNFIQQFPDTYWEG
                     GMFVFDDRRVFEANTKEELKYTAKCHFCGKPTSYHINCHNIDCDKIIVCCHDCKVEKE
                     YCCSDECRSSSN"
BASE COUNT          859 a          306 c          428 g          690 t
ORIGIN      
        1 aatagaagat cgtgtaaaaa cacttatgtc cgagttagat aaatcgactt ctgaagttga
       61 gtatttaaat aaaaagttac atgatgtatt taaggataaa atatccgaac tcaattttga
      121 aatcaatcaa agaaaaaaag ctgagttatc actgcaaaaa agtgaggaac aatttagact
      181 cgtatttcag aatgcaccta tagggattgt gattatatcc acagaaggaa aaattataag
      241 tgttaataaa tccttttgtg atacaatagg atttgaaaga gacgaaatta tcggaatacc
      301 tatcaaatat ctttttgagc agaatgattt agaaggcttt agtgagggta cgctagataa
      361 caatggaaat cctgttgctg atattaatac tgaaaaaatg ctgcttaaaa aagagggtaa
      421 ggaaattcat gtaattgtaa aatcagtttc tgttcttgat gagaatggaa aagtaaaaca
      481 ttatgttatg cagctgcttg atatttccga gattaaaaga gtacaacggg aattggttga
      541 tgctcttaat aaggcagaag aatcggatag acttaaatca gcctttcttg ctcaaatgtc
      601 acatgaaatc aggactccgc ttaatttaat attaacttca ataccattga ttgcagatga
      661 aatttccagt gaggatgagg atctaaaaat tattttagat tctgtaaaaa gtgctggcag
      721 aaggttgcaa agaacaatag atatgatatt aagtatgtca tctgttcaaa gcggcaacta
      781 caaagctact tttgaaaaat ttgatattgt tcctgactta caaaacatgg ttaaagaatt
      841 caaatcttta agtgacgata aaggattaaa gcttaaattt tcgcagagcg atgattcagc
      901 atttattact gccgacagat atacagtcaa tcaggttttc caaaatctta tcaataatgc
      961 tatcaagtac actttaaaag gatatgttga agtgtatgtt aaaaatctac agaatgataa
     1021 agttaaaatc gagatcagag atagcggcat tggtatgagc gaggaatatc ttcaaagaat
     1081 gtttactccg ttttcacagg aagataccgg atacaaacgc gaatttgaag gtaacggctt
     1141 gggtttagca ctcgttaaaa aatacataga acttaaccgt gctgaaataa gcgttgagag
     1201 tgaaaaaaac attggttctg tgttttcggt tgtatttgat agagaggtaa cctttacaac
     1261 acccgtagaa tctgaaaaaa gatataaatc ttacaattaa aaattattca actattctta
     1321 tttcaattcc cttctaaatt aattttatcc ttaaaaactt ttttataaaa gattatattt
     1381 gtgttacaat aaataccaca aaaaattatg cgaaattaaa tgagcaaata caaagttata
     1441 tcgttctata aatacataaa cattgaaatt ccggaagaat tggctaagga gcatcttaat
     1501 tggtgtttag aaaatggaat taaaggaaaa gtttatttcg ctaaagaagg gattagtggc
     1561 tctatttttg gtgataatga agttacacgt aaatataaga atcatttaaa aagtcacaat
     1621 atctttgaag atatttggtt taaagaaaca ccaactgacc gggtagcatt taataaaatg
     1681 catgttcgag ttaaaaacga aattgtaaat tcgggattgg caggaacttc acttgaacat
     1741 accgcaccca gattaactcc ggagcagctg ttaaaatttt atgaaaacaa aaaggagttt
     1801 gtaatagttg atgcacgaaa ctggtatgaa agtaagattg gaaaatttaa aaatgctatc
     1861 actccgcaaa taacacattt tagagaatgg cccaaagtag ttgagtcatt aagtgaatac
     1921 aaagataaaa caatagttac ttattgcacc ggtggaataa gttgcgaaaa agcttctgct
     1981 tatatgcgcg aacagggctt taaagatgtt tatcaaatcg atggcggaat tttaaacttc
     2041 attcagcagt ttcctgatac atattgggaa ggcggaatgt ttgttttcga tgatcgcaga
     2101 gtttttgaag cgaacacaaa agaggaattg aagtacacgg ctaaatgcca tttttgtggt
     2161 aaaccgactt catatcatat aaattgtcac aacattgatt gtgataaaat tattgtttgc
     2221 tgccacgatt gtaaagttga aaaagaatat tgctgtagcg atgaatgcag atcaagttca
     2281 aat
//
LOCUS       DASWBY010000060         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_2440, whole genome shotgun sequence.
ACCESSION   DASWBY010000060 DASWBY010000000
VERSION     DASWBY010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2440"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VF870_01295"
     CDS             <1..1085
                     /locus_tag="VF870_01295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9250840.1"
                     /translation="SQYFILGPEVSAMEKEFCNYLGCKHALGVSSGTDALLLALMAID
                     IKPGDEVIVPTYSFFATAGVVSRLFATPVLVENDPVTFNIDPKDFEKKITPKTKAVIP
                     VHLYGQSAELDEIVNIAKSHNIIVIEDAAQAIGTQYKDGRFVGTIGDIGCFSFFPSKN
                     LGCYGDGGLVTTNNDELAKILTIKRVHGGEPKYYHKVIGGNFRIDALQAAVLRVKLPH
                     LDGWSEKRRHNANRYTQLFIEAGLAEETGKISFDANNKVLLPKAVYEKSGAKNYHIYN
                     QYIVRVDKRDEMRDFLTKSEIGTEIYYPVPFHLQECFKDLGHKKGDFPISEFSADTSI
                     ALPIYPELSDEQLIYIVETFKKFFNQ"
     gene            1091..2746
                     /locus_tag="VF870_01300"
     CDS             1091..2746
                     /locus_tag="VF870_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250841.1"
                     /translation="MILKKIFLFLVFSSIAFAQVVYEPLHRDVYKFLSRLSQKSVIVF
                     DDQIRPLSRKYIAQKLIEAAEKSFELTKLEKDELGFYSKDFKFEFDMINNVKIDSSEI
                     TIVGYDAGDRLRLFGYRNNFFSLNLSPILGYKMGSRDNQKLTHFWNGIYTYGYIDKYI
                     GFSFDFRDNTEAGNTIDKTKEFTPVTGVEAKSSLTAPLYANDKMEYSESKAVLATDWS
                     WGSFAIGKDFLEWGYGESGLLVLSQKPPSYGFIRLDIKPVDWLKFNYMHGWLSSGVID
                     SNSIFYNTSGGISFSFIDKFIATHSLIITPVSGIDISIGESIIYDDKLEFLYLMPIMF
                     FRLADHQISEQENAAGGNAQFFLGVSSKGQIKNTHLYGTLLIDEISLNGLFDPETQRY
                     QMGFSLGGSITDLPIENLTAKIEYTKIYPFVYQHYVQTTDYKSADYILGHWMGHNADL
                     IYGSLNYRFLRGLLATVWGQYIRKGEDGNVQQQYNTIPQPPFLFGLRKNYSYIGAEIK
                     YEYTHELFAKFNFQTTLSSIQQEDLSFIDTTLNEFYFSIYYGL"
     gene            2752..3708
                     /locus_tag="VF870_01305"
     CDS             2752..3708
                     /locus_tag="VF870_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX9250842.1"
                     /translation="MDDLINKKVYLAGHTGMVGSAIFRELENCGYKNFIYKDFTELDL
                     RSQSEVLNFFSQTKPELVIIAAAKVGGILANNTYRAEFLYDNLMIEANLIHASYLNNV
                     EKVVFLGSSCIYPKLAPQPLKEEYLLTDTLEFTNEPYAIAKIAGIKLCENYYRQYGCN
                     YISAMPTNLYGRNDNFNLETSHVLPALIRKFHEAKVGKKESVIIWGSGKPLREFMYVE
                     DLANAIVFMMENIEAKELYEKGITHLNVGTGKDITIAGLSVLISEIIGYNGKIEYDSS
                     KPDGTPRKLMDVTRINSLGWKYKTELKEGIIKTYNFFKQKHL"
     gene            3715..4839
                     /gene="gmd"
                     /locus_tag="VF870_01310"
     CDS             3715..4839
                     /gene="gmd"
                     /locus_tag="VF870_01310"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX9250843.1"
                     /translation="MKALITGITGQDGSYLTEILLEKGYEVHGIIRRSSSFNTGRIDH
                     LYENPEILNKKLFLHYGDLVDTSNLNRLLEKIQPDEIYNLAAQSHVKVSFEVPDYTAQ
                     VDALGTLRFLDAIREVGLRKVKFYQASTSELFGKVQEVPQSEKTPFYPRSPYGVAKLY
                     GYWIIVNYREAYDLFACNGILFNHESPKRGETFVTRKITRAASRIAAGLQSDLSLGNL
                     DAKRDWGYAPEYCEGMWRILQYKKAEDFVLATGQTNTVREFVELSFKHLGVDLEWKGK
                     GVNEKGVISKIDFEKAKSLIEFKKNSKLYKFDFTNKLKKGNTVVTVNHNYYRPTEVEL
                     LIGNPTKAKKLLGWKAKTKFEDLVQIMIKSDMDKVLRRGY"
BASE COUNT         1686 a          745 c          950 g         1538 t
ORIGIN      
        1 aatcccagta ttttattttg ggaccagaag tatctgcaat ggaaaaagaa ttttgtaatt
       61 atcttggatg taaacatgca ctcggagttt cttcgggtac agatgcttta ctattagctt
      121 tgatggcaat agatataaaa cccggcgatg aggttattgt tccaacttat tctttctttg
      181 ctacggcagg agttgtttca agattattcg ctactcccgt tttagttgaa aatgatcctg
      241 ttacttttaa tatagatcca aaagattttg aaaagaaaat aacacctaaa actaaagcag
      301 ttattccagt tcatttatat gggcaaagtg ctgagttaga tgaaatagtc aatattgcaa
      361 aatcacataa cataatcgta attgaagatg ccgcacaggc aatcggtaca caatacaaag
      421 atggaagatt tgtcgggaca attggtgata ttggatgttt ctcgttcttt ccaagtaaaa
      481 accttggctg ctatggtgat ggcggattag ttacaacaaa caacgatgag cttgcaaaaa
      541 ttcttacaat aaaaagagtc cacggcggcg aacccaaata ttatcataaa gtaatcggtg
      601 gaaattttag aattgatgct ttacaagccg cagtacttcg ggttaaactt ccccatttag
      661 atggctggtc agaaaaaaga agacataatg caaatcgtta tacgcagctt tttattgaag
      721 cgggtttagc tgaggaaacc gggaaaataa gttttgatgc aaataataaa gttctgcttc
      781 caaaagcagt ttatgaaaaa tccggtgcaa aaaattatca catctacaat caatatatag
      841 ttcgtgttga taaacgtgat gagatgagag actttttaac aaagagtgaa atagggacag
      901 aaatttatta tcctgttcct ttccatctac aggaatgttt taaggatctt ggacacaaga
      961 aaggcgattt cccgatttca gagttttcgg ctgatacatc aattgcttta cctatttatc
     1021 cggaacttag cgatgaacag ttaatttata ttgttgaaac ctttaaaaag ttctttaatc
     1081 aataagttaa atgattttaa aaaagatatt tctgtttctt gtttttagct ccattgcatt
     1141 tgcgcaggtt gtttacgaac ctttacacag agatgtttat aaattcctct caaggcttag
     1201 tcaaaaatct gtaattgttt ttgatgacca gattagaccc ttaagcagaa aatatatcgc
     1261 acaaaaactt attgaagcag cagaaaaatc ttttgaatta accaaactgg aaaaagatga
     1321 gcttggtttt tatagcaagg attttaaatt cgaatttgat atgattaata atgtcaaaat
     1381 agactcctca gaaattacaa tagttggtta tgatgcagga gatcgactta gattatttgg
     1441 ctatcgaaat aattttttca gtttgaattt aagtccaatt ttaggatata aaatgggttc
     1501 ccgtgataac cagaagctaa cacatttctg gaacggaatt tacacttatg gctacattga
     1561 taaatatatt ggctttagct ttgatttcag ggataacact gaagctggaa atacaattga
     1621 caaaaccaaa gagtttactc ccgttactgg cgttgaggca aaatctagtt taactgctcc
     1681 gctatatgct aacgataaaa tggagtacag tgaatcaaaa gcggtattgg ccactgactg
     1741 gagctggggt tcgtttgcga ttggtaagga ttttttagaa tggggttatg gtgaaagcgg
     1801 actgttagtg ctttcacaaa aaccaccatc ttatggattt ataaggctcg atattaaacc
     1861 ggttgattgg ctaaaattta attatatgca cggttggctt tcttcgggcg taatcgattc
     1921 aaattcaatt ttttataata cctccggtgg tatcagtttt tcttttattg ataaatttat
     1981 tgccactcac tcattaatta ttactccagt ttcagggata gatatatcta taggagaatc
     2041 aattatttac gatgataaac ttgagttttt atatcttatg ccaattatgt tttttagact
     2101 ggccgatcat caaattagcg aacaggaaaa tgccgcaggt ggcaatgcgc aatttttctt
     2161 gggtgtcagt tcaaaaggac aaataaaaaa tacacatctg tatggaacat tattgataga
     2221 tgaaatttca ttaaacggac tatttgatcc tgaaactcaa cgatatcaaa tgggatttag
     2281 ccttggggga tcaattaccg atttaccaat tgaaaatctt actgcaaaaa tagagtacac
     2341 taaaatttat ccgtttgttt accagcatta tgtacaaact accgattata aaagcgctga
     2401 ctacattctt gggcattgga tggggcataa tgctgatctg atttatggtt ctctcaatta
     2461 ccgatttctg cgaggtttgc tggcaactgt ttggggacaa tatattcgca aaggtgagga
     2521 tgggaatgtt caacagcaat acaacactat acctcagccg ccctttttat ttgggctgag
     2581 aaaaaattat tcatacattg gtgcagaaat taagtacgag tatacacacg aactttttgc
     2641 taaatttaat tttcagacaa cactttcttc aatacaacag gaagatttaa gttttattga
     2701 tactacgttg aatgaatttt atttttcaat ctattatgga ttataatttt aatggatgat
     2761 ttaataaaca aaaaagttta tttagcaggt cataccggaa tggttggatc tgcaatattt
     2821 agagaattag aaaattgtgg atataaaaat tttatctaca aagattttac tgaactcgat
     2881 ttacgcagtc aatcagaagt tctaaatttc tttagtcaaa caaaaccaga attagtaatt
     2941 attgcagcgg caaaggttgg gggtatactt gcaaacaata cttatcgcgc agaatttctt
     3001 tacgacaact tgatgattga agctaatctt attcacgcat cgtatctgaa taatgttgag
     3061 aaagtggttt ttcttggaag ctcctgcatt tatccgaagc ttgccccgca gccattaaaa
     3121 gaagaatatt tgcttacgga tacgcttgag tttaccaatg aaccatacgc cattgctaaa
     3181 atagcaggaa taaaactctg cgagaattat taccgtcagt atggatgtaa ttacatttcg
     3241 gctatgccga caaacttata tgggcgaaat gataatttta atcttgaaac atctcatgtt
     3301 cttcctgcgt tgatcagaaa atttcacgaa gcaaaagttg ggaaaaagga gtcagttata
     3361 atctggggat caggaaaacc tttacgtgag tttatgtacg tagaagattt agccaatgca
     3421 attgttttta tgatggagaa cattgaagca aaagagcttt atgaaaaagg gattacacat
     3481 ttaaatgttg gaacaggaaa agacattacg atagcaggtt tatccgtatt aatttcagaa
     3541 attattgggt ataatggtaa gattgaatat gattcatcaa agccggacgg aacaccaaga
     3601 aaacttatgg atgttacaag aataaactcg cttggctgga agtacaaaac tgaattgaaa
     3661 gaaggaatta ttaaaacgta taatttcttt aaacaaaaac atttataggg aaatatgaaa
     3721 gctttaataa ctggaataac cggacaggat ggcagttact tgactgaaat tcttcttgaa
     3781 aaaggatatg aggttcacgg aattataaga agaagcagtt cttttaacac aggaagaatt
     3841 gatcatcttt atgaaaatcc tgaaatactt aataagaaat tattccttca ttatggcgat
     3901 cttgttgata caagtaactt aaatcgttta cttgaaaaaa ttcaacctga tgaaatttac
     3961 aatcttgcag cacaaagcca cgttaaagtt tccttcgaag ttccggatta cacagcacag
     4021 gttgatgcac ttggtacttt gaggtttctt gatgcaatcc gtgaagttgg attaagaaaa
     4081 gtaaaatttt atcaagcatc aacaagtgaa ttgtttggaa aggtgcagga agttccgcaa
     4141 agtgaaaaaa ctccttttta tccccgctca ccttatggtg ttgccaagct ttacggctat
     4201 tggataattg taaactatcg tgaggcatat gatctttttg cctgtaacgg aatattgttc
     4261 aatcacgaat caccaaagcg cggtgaaaca tttgtaacaa gaaaaataac tcgtgctgct
     4321 tcaagaattg cagcaggact tcagtcagat ctttctctcg gtaacctgga tgcaaaaagg
     4381 gattggggtt acgcgcccga atactgtgaa gggatgtgga gaattcttca gtacaaaaaa
     4441 gcggaagatt ttgtgttagc aacaggacag acaaacaccg taagggaatt tgtagaatta
     4501 tcgttcaagc atcttggtgt tgatttggaa tggaagggca aaggcgtaaa tgaaaaaggg
     4561 gttatcagca aaattgattt tgagaaagcc aaatcactta tagaatttaa aaagaattca
     4621 aagctgtata agtttgattt tacaaataaa cttaaaaaag gcaatacagt tgtaacagta
     4681 aatcataact attatcgtcc tacagaagtt gagttgttaa ttggtaatcc aacaaaagca
     4741 aaaaaacttt tgggttggaa agcaaaaaca aagtttgaag atcttgttca gataatgatt
     4801 aaatctgata tggataaagt tttaagaaga ggttattaga tagtaaaata agttttattc
     4861 aataaacgtt agaggtaaac atgttcgatg caaattataa attcacctgt gtagataga
//
LOCUS       DASWBY010000061         3558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_265575, whole genome shotgun sequence.
ACCESSION   DASWBY010000061 DASWBY010000000
VERSION     DASWBY010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265575"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VF870_01315"
     CDS             complement(<1..362)
                     /locus_tag="VF870_01315"
                     /inference="COORDINATES: protein motif:HMM:NF027451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HEX9250844.1"
                     /translation="MKKILIVLSLFGFIYLINGCDSSKKPATGFEDEIYVVADSSEYE
                     ELKVALQAAFEVEINTPMPEKLFTLKRISSNLIEKVKNKKNIVIVAPLNSNSYTSQYI
                     KSIVDSAIQKRLETENDF"
     gene            complement(365..1033)
                     /locus_tag="VF870_01320"
     CDS             complement(365..1033)
                     /locus_tag="VF870_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichol kinase"
                     /protein_id="HEX9250845.1"
                     /translation="MTHIDNGTIHYRDELDRKLIHLFSLSIPVIYYFIPSSTSITILI
                     CLTVFALIIDGGRFISKSFANIFYKTFGFLLRKHELDKEKKNLTGATYVLLSALICAL
                     IFPKVIFVTAFTILIISDTMAALIGRKFGKRKFLRKSFEGTLSFFISASIVVFLTPKV
                     GNFTMEYMIGFIAAFVGAIVENISYGFADDNLSIPISVGLTMWVLYLLIFPNLDLILQ
                     NVPR"
     gene            complement(1020..3065)
                     /gene="ftsH"
                     /locus_tag="VF870_01325"
     CDS             complement(1020..3065)
                     /gene="ftsH"
                     /locus_tag="VF870_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9250846.1"
                     /translation="MSDNEKKSGNKNPKDNKPNRPDDNFDWSKVIRMVFGWGAVIVAA
                     VIVMQVFRTSAETYTEIPYGQYEILLENPENISSASITKADLNDYTFKAELKKETNIT
                     IKGKSVSVKAVSVYIPEPMIRDQEAIWKAKGIQYSFDKESNEWLNILIGFLPWVLIIA
                     VWVIIMRRMQGQGGGSRGIFSFGKSKAKLITPSSKRVTFKDVAGTDEAKMELQEIIEF
                     LKEPTKFQKLGGKIPRGVLLLGPPGTGKTLLARAVAGESGVPFFSISGADFVEMFVGV
                     GASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNALLVEMDGFE
                     QNSGVIIIAATNRPDVLDPALLRPGRFDRQVVVDRPDVKGREGILKVHTRNIPLGETV
                     DVEVIAKGTPGLAGAELANLVNEAALLAARKNKKKVEMIDFEEAKDKVMMGMERKSLI
                     ISEKEKKTTSYHEIGHVLVARMIPEADPVHKVTIIPRGRALGVTSYLPIDEKHTYSKE
                     YLEAMITYALGGRAAEKLVFNHYTTGAGNDIEKATNIARKMVCEWGMSDKLGPLAYGA
                     KEEEIFLGREIQKHRDYSEKTAIEIDDEIRGIINSAFERAVKILSDNMELLHRLSEEL
                     LEREILDAEEIDRIIRGENLPPIQKEIKTAEEAVPDHVKKLMEQRQQQDTAPKNDSH"
     gene            complement(3107..>3558)
                     /gene="hpt"
                     /locus_tag="VF870_01330"
     CDS             complement(3107..>3558)
                     /gene="hpt"
                     /locus_tag="VF870_01330"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX9250847.1"
                     /translation="ISQDYVGKIPIFIGVLNGAFIFLSDLIKNISIDCEIDFFKLSSY
                     GDSKISSGNVRLLKELNCDVNGRDIIIVEDIVDSGLSIKYIEEIFVKYTPSSMKVCSL
                     LMKPESIKYNVKIDYIGFNIPGKFVIGYGLDYAQKFRNLRSIYTLSE"
BASE COUNT         1105 a          707 c          512 g         1234 t
ORIGIN      
        1 ataaaatcgt tttctgtttc taatcttttc tgaattgcac tgtcgacaat tgatttgatg
       61 tattgtgaag tgtaggaatt agagtttagc ggagctacaa ttacaatgtt ttttttgttt
      121 ttaacttttt caataaggtt agatgaaatt ctctttaaag taaaaagttt ttcaggcatc
      181 ggcgtgttta tttcaacttc aaatgcagcc tgcagtgcta cttttagttc ctcatattcc
      241 gatgagtctg caactacata aatttcatct tcaaatccag tagctggttt ttttgatgaa
      301 tcacatccat ttattaaata tataaagcca aataacgaga gaacaattaa aatctttttc
      361 ataattacct cggaacattt tgtaagatca aatctaaatt gggaaatatt aacaaatata
      421 aaacccacat agtcaatcca acggagattg gaattgataa gttgtcatca gcaaatccat
      481 atgaaatatt ttcaactata gctccaacaa atgcagcaat aaaaccaatc atgtattcca
      541 tcgtaaaatt accaaccttg ggtgttagaa aaaccactat gcttgcacta atgaaaaaag
      601 ataaagttcc ttcaaaactt tttcgcagaa attttctttt gccaaacttt cttccaatta
      661 atgcagccat tgtatcactt ataatcagga tagtaaaggc agtaacaaaa atcactttag
      721 gaaaaattaa agcgcaaatt aatgctgata agagcacata agtagcacct gttaagtttt
      781 tcttttcttt atccagttca tgttttctta acaaaaatcc gaaagtttta taaaaaatgt
      841 ttgcaaatga tttagagatg aaccttccac catcaatgat aagagcaaat acagttaaac
      901 agataagaat tgttatcgat gtagaggatg gaataaaata atagataaca ggaatagata
      961 aagaaaagag atgaattagt tttctatcta attcatctct gtaatgaatt gttccattat
     1021 caatgtgagt catttttcgg tgctgtatcc tgttgttgcc tttgttccat tagttttttt
     1081 acatggtcag gaacagcttc ttccgctgtt ttaatttctt tttgaattgg tggtaaattt
     1141 tcaccacgaa taattctgtc tatttcttca gcatcaagaa tctctctttc aagaagttct
     1201 tcagacaacc tatgaagtaa ttccatgtta tcactaagta tttttacagc tctttcaaaa
     1261 gcggaattaa ttatacctcg aatttcatcg tcaatttcaa ttgctgtttt ttcgctatag
     1321 tctctgtgct tttgaatttc tctgcctaaa aatatttctt cttcctttgc accataagca
     1381 agaggaccaa gtttatcact cattccccat tcacaaacca tcttacgtgc aatgtttgtt
     1441 gctttttcaa tatcgtttcc ggcaccggtt gtgtaatgat tgaataccaa tttctcggca
     1501 gctcttccgc ccaatgcata tgtaatcatc gcttctaggt actcttttga ataagtatgc
     1561 ttttcatcga taggcagata acttgtaaca ccaagtgctc ttccgcgtgg aatgatagta
     1621 actttgtgca caggatctgc ttcaggaatc attcttgcaa caagtacgtg accaatttca
     1681 tgataagagg tagttttctt ttctttttca gaaattatca aactctttct ttccattccc
     1741 atcattactt tatctttagc ttcttcaaag tcgatcattt caactttttt cttattcttt
     1801 ctggcagcta atagtgcggc ctcattaaca aggtttgcta attctgcacc cgctaatcct
     1861 ggcgttcctt tagctatcac ttctacatcc acagtttcac cgagaggaat atttcttgtg
     1921 tgaactttaa gaattccctc tcttccttta acatcaggac gatctacaac aacctgtcta
     1981 tcaaatcttc ccggtctcaa taatgccgga tcaagaacat caggcctgtt tgtagctgcg
     2041 ataattatta caccgctgtt ttgctcaaat ccgtccatct caacaagtaa cgcattcaga
     2101 gtttgttcac gttcatcatg tccgccgcct aaacctgcac cacgatgtct tccaaccgca
     2161 tcaatttcat caataaaaat tatacaagga gcattctttt ttccctgttc aaataaatca
     2221 cgaactcggc ttgcaccgac accaacaaac atctcaacaa aatctgctcc tgaaatcgaa
     2281 aagaatggga caccggattc tcctgctaca gcacgagcta ataaagtttt acctgttccg
     2341 ggaggtccta ataataaaac tcctctcgga attttaccac caagcttttg aaacttggtt
     2401 ggctctttaa gaaactcaat aatctcctgg agttccattt tagcttcatc agtaccggca
     2461 acatctttaa atgtaactcg tttgctggat ggagttatta gctttgcttt actttttcca
     2521 aaagaaaaaa tacctcttga tccgccgcct tgtccctgca ttcttctcat tattataacc
     2581 caaacagcaa tgataagtac ccacggcaaa aatccaatta aaatattcag ccattcattt
     2641 gattctttat caaaagaata ttgaattcct ttagctttcc aaattgcttc ctgatcacga
     2701 atcataggtt cgggaatgta aactgaaaca gctttaaccg aaaccgattt gcctttgatt
     2761 gtaatgttag tttctttttt aagctcagct ttaaaagtgt aatcatttaa atctgctttt
     2821 gtaatgcttg cggatgaaat attttccgga ttctcaagaa gaatttcata ttgaccatat
     2881 ggaatttctg tataagtttc tgcgctagtt ctaaaaacct gcataacaat tacagcagct
     2941 actataactg caccccagcc aaaaaccatt ctgataacct ttgaccaatc aaaattatca
     3001 tcaggtctgt ttggtttgtt atctttaggg tttttattac ctgatttttt ctcgttatca
     3061 ctcatattat catttctaga attttattca acaattaatt tttattttat tcacttagag
     3121 tgtaaataga cctaagattt ctgaattttt gggcataatc taagccatag ccgattacaa
     3181 atttacctgg aatattaaac ccaatataat caattttaac attatattta atgctttcgg
     3241 gcttcattaa cagcgagcaa accttcatac tactgggtgt gtatttcaca aaaatttctt
     3301 ctatatactt aatagagagt cctgaatcaa caatatcctc aacaataata atatcacgtc
     3361 cgttcacatc gcaatttagt tctttgagta agcgcacatt acctgatgat attttagaat
     3421 cgccataact ggagagctta aaaaagtcta tttcacaatc gattgaaata tttttaatca
     3481 aatcggatag aaaaataaaa gcaccgttaa gaactcctat aaagattgga atttttccaa
     3541 cataatcctg tgatattt
//
LOCUS       DASWBY010000062         5605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_416237, whole genome shotgun sequence.
ACCESSION   DASWBY010000062 DASWBY010000000
VERSION     DASWBY010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416237"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VF870_01335"
     CDS             complement(<1..736)
                     /locus_tag="VF870_01335"
                     /inference="COORDINATES: protein motif:HMM:NF036497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX9250848.1"
                     /translation="MNNLKRFFLKYRLNIASGLTILLAFVAVLSIYFSLEVRLQSNDE
                     CLWLPKKVVKDSTIIIFDKVKVDGVSWNAGIRNGDQLLQINSIPLKNTLQAQEILNKY
                     QSGELADYVVKKKSGEILQTKVYVKKLVQFGSLASAISGLIFLLIGFIVYSAKPDGLA
                     QRIFYSLGVFTVLSAAVVLFPLDDLFFMKFITEQPKTALITGSIVIISQVFVQFLVLY
                     FVWSFPVQFKFASKKSVKIALIASAII"
     gene            complement(769..2448)
                     /locus_tag="VF870_01340"
     CDS             complement(769..2448)
                     /locus_tag="VF870_01340"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX9250849.1"
                     /translation="MQDNDSLKVKRNLTALVEFSRVINSSLDLNFILNNVLLTCLGKF
                     LAIRGIIALNINGKLKIKSYKGVAEENILLFPDMNASEDCIENDELKKFFVDNNLIAS
                     EKISSSNECVGIVCLGEKLNKQDYSKDDLDFLKTILNISASAIQNSLIVEELKFVNRE
                     LDSKIQRLSSLFELSKEFGLFSESTKVTRLLIFSLIGQFLVQKYALLRFEGNDIEVIE
                     PKLPVEELLSNLRKIDYHRIDSSLNKEQVALLFPALYEMGIEVIVPMQIQGKTKGLII
                     LGRRINNIDYTDADIEFIFSVGSLAIISMENRRLFREALEKQKMEEELDLAREIQRNL
                     LPQKIPVYNNFNIAAVNVSSKQVGGDYYDIIELDENRCCVAIADVSGKGVPASLLMAN
                     MQAFLQVICRQNISLDIATGLINDLISKNTADGKFITFFWGILNNADKTFKYVNAGHN
                     LPLLIRDGKIIKLGIGGMILGVMKTIIPYRSETVQLLKDDVIVMFTDGISEAMNKKGE
                     EFSDESLEKLSLESTGDTSENIIKKIQGAVQKFTDGANQSDDITLVVVKVK"
     gene            complement(2438..2875)
                     /locus_tag="VF870_01345"
     CDS             complement(2438..2875)
                     /locus_tag="VF870_01345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9250850.1"
                     /translation="MKTKQLKVKSKTENLSVIRDFISTSAAEAGVATDTVENIILAVD
                     EACTNIIRHAYKSFPDGELIIKTKSTLSRFIVSITDYGKSFAPEMIPEPDLQKYYRQR
                     RVGGLGMYLMKTLMDDVKYVSVPGKYNEVLLSKNIKVVRSNAG"
     gene            complement(2877..3230)
                     /locus_tag="VF870_01350"
     CDS             complement(2877..3230)
                     /locus_tag="VF870_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9250851.1"
                     /translation="MAEFNTSIKNQGAVSIINLKGYLDAHTAPILENNFVDLIDKNCY
                     QIVVDFKDLAYISSAGLGVFMAYIEKIRDSKGDIKLSGMSDKVYNIFDLLGFPLLYEI
                     FKSEEEAVKKFSETK"
     gene            complement(3188..5131)
                     /locus_tag="VF870_01355"
     CDS             complement(3188..5131)
                     /locus_tag="VF870_01355"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX9250852.1"
                     /translation="MKLLARKNPHPSALVLNIGIICAFIFLVIMFAENILNVQIQKLS
                     DKPVNQGIFENLVNTFYGLMFLGIASYWFASLKELYFYKQYRRSSTYFITMSVFIAIS
                     AVSNLLFRSTEYEFIYNTFLIISIILISFNSLKISWIAFINKKEKISLLILSIIISAL
                     FIINLINSGKTDFNGTILMSFSPTTFQFFNLILLYGGIYFAVLFFTTLFHIPTAEAYD
                     RKANEVSSLQYFSKLITQVLDIEDLAETITEITTKVSSADAAWIILKENSDRKILANK
                     NIALVDADLINQFLLESGICENITETKICSLQKFINKSQLSEKFGSIAVSPLRSYNEI
                     KGYLIAARKNELIFYEEDKTAINTFSDYASVAIENSLLLEQSIEKERLAKELDVARDI
                     QKKILPAKDPVFEDLSITSVFIPAFEVGGDYYDFFEISKNKLGFIIADVSGKGISAAF
                     IMAEVKGIFSSLSRMIENPKDILIKANEILESTLNKKNFVSALYGIIDFDNNVLSFSR
                     AGHCPALLVRNNSIETFKPSGIGLGLANDTLFRNHLEEIKIDLKQNDTLVFYTDGITE
                     AKNKELEDFGEQRFADILIQYSSESVNEIANKVIKDVTLFSRNHSQYDDITLVILKWN
                     KKNNLNGVKEWQSSTPQLKTRVL"
BASE COUNT         1894 a          974 c          790 g         1947 t
ORIGIN      
        1 agataattgc tgaagcaatt aaagcaatct ttacactttt tttggatgca aatttaaatt
       61 gtacaggaaa ggaccacaca aagtatagaa ctaaaaactg cacaaatacc tgactgatga
      121 taacaatact tcctgttatt aatgcagttt tgggttgttc agtaataaac ttcataaaga
      181 ataaatcatc gagcggaaat aaaacaactg ctgccgatag tactgtaaat acgccaaggg
      241 aataaaatat tctttgggcc aagccatccg gctttgcact gtaaacaata aatccaatta
      301 aaagaaaaat taatccactg atggctgaag caagtgagcc aaattggaca agttttttta
      361 catagacttt tgtttgtaat atctctccac tttttttctt aacaacatag tcagctaatt
      421 caccagattg atatttgttt aatatctctt gagcctgaag agtattttta agaggaatag
      481 aatttatttg taaaagttga tcaccattcc gaattccagc attccatgaa actccatcaa
      541 ctttaacttt atcaaaaata attattgtag aatcctttac tactttctta ggaagccata
      601 agcattcgtc atttgattgt aatctaactt ccagagaaaa ataaattgaa agtactgcta
      661 caaaagccag aagaattgtt aatcctgaag caatgtttaa tctatattta agaaaaaatc
      721 ttttaaggtt attcatcgta ttcttacttt gattttaagt ccacaattct atttaacttt
      781 aacaactact aaagtaatat catcagattg atttgcgcca tcagtaaatt tttgaactgc
      841 gccctgtatt ttttttataa tattttcaga tgtgtccccg gttgattcca aggataattt
      901 ttccaatgat tcatcagaaa actcttcacc ttttttattc atagcttcag aaatcccatc
      961 agtaaacatc acaattacat catcttttaa caattgtact gtttcagatc tatagggaat
     1021 tatagttttc ataacgccca gaatcattcc gccaatacca agtttaataa tctttccatc
     1081 gcgaataagt aaaggaagat tatgtcctgc gtttacatat ttaaaagttt tatcagcatt
     1141 gttcagaatt ccccagaaga atgttatgaa ttttccatcc gcagtatttt ttgatataag
     1201 atcatttatt aatccggtag caatatcaag tgaaatattc tgtctgcata ttacttgtaa
     1261 aaacgcctgc atattagcca tcagcaaaga agccggaaca cctttacctg atacatccgc
     1321 gatagcaacg caacatctat tctcatcaag ttcaataata tcgtaataat ctccgccaac
     1381 ttgttttgat gagacattaa cagcagctat attaaaatta ttataaactg gaatcttttg
     1441 tggtaaaaga tttctttgaa tctcacgtgc aagatccagt tcttcttcca tcttttgttt
     1501 ttctaatgct tctctaaaaa gtcttctgtt ttccattgaa atgattgcaa gacttcctac
     1561 tgaaaaaata aattcaatat cagcatctgt gtaatcaatg ttgttaattc ttctaccaag
     1621 gataataagc ccctttgtct ttccctgaat ttgcattggg acaatgactt ctatccccat
     1681 ttcatataat gccggaaata ataatgcaac ctgttcttta tttaaagagc tatcaattct
     1741 atgataatca atttttctaa gattgcttaa cagttcttca acaggtaatt tgggttcaat
     1801 tacctcaata tcatttcctt caaatcgaag tagggcatat ttttgaacta gaaactgacc
     1861 gattaaagaa aagataagta aacgggtaac tttagtcgat tctgaaaata gcccaaactc
     1921 tttacttagt tcaaacaaag aacttaacct ttgtattttt gaatcaagtt cacgattaac
     1981 aaatttcaat tcttcaacta ttaaagagtt ttgaattgca gatgcagaga tatttaaaat
     2041 tgttttaaga aaatcaagat catcttttga ataatcctgt ttgttaagct tttcacccaa
     2101 gcaaacaata ccaacacact catttgatga actgattttt tcagaggcaa tcagattatt
     2161 atcaacaaag aattttttta attcatcatt ttcaatacaa tcttcacttg cattcatatc
     2221 agggaaaagt aaaatatttt cctcggcaac tcccttatat gatttgattt taagtttgcc
     2281 attgatattt aaagcaatga ttcccctaat agcaaggaat ttgccaaggc aggttaacag
     2341 aacattattg agaataaaat taagatcaag gcttgaatta attaccctgc tgaattcaac
     2401 aagagcggta agattacgtt ttacttttaa actatcatta tcctgcattg cttcgtacta
     2461 cttttatatt ttttgatagc agcacttcgt tatattttcc aggaacagac acatatttaa
     2521 catcatccat aagtgttttc attaaataca ttcctaaacc accgactctt ctttgtcgat
     2581 aatacttttg caaatctggc tcggggatca tttcaggtgc aaaagatttt ccataatcgg
     2641 taatgctaac tataaaacga gaaagtgtag attttgtttt gataatcaat tccccatcag
     2701 gaaaagattt ataagcatgt ctaataatat tagtacaagc ttcatccaca gcaagaatga
     2761 tattttctac agtatcagtc gctacccctg cttcagcagc agaagtgctt ataaaatctc
     2821 tgatgaccga aagattttca gttttgcttt ttacttttag ttgttttgtc ttcactttat
     2881 tttgtctcag aaaatttctt aactgcttcc tcttcagatt taaaaatctc gtaaagcaaa
     2941 ggaaatccta aaagatcaaa aatgttataa actttatcac tcatacccga aagcttaatg
     3001 tctcccttgc tgtctctgat tttttctatg tatgccataa atactcctaa accagcactg
     3061 cttatgtaag caagatcctt aaaatcaacc acaatctggt aacagttctt atcaataaga
     3121 tctacaaaat tgttttcaag gattggcgca gtatgtgcgt caagatatcc cttaaggttt
     3181 attatactta cagcaccctg gtttttaatt gaggtgttga actctgccat tctttaactc
     3241 cattcaaatt atttttttta ttccacttta atataaccaa agttatatca tcatactgag
     3301 agtggttacg tgaaaataat gttacatcct taataacttt attagcaatt tcatttactg
     3361 attcagaaga atattgtatt aatatatcag cgaatctctg ctcgccaaaa tcttctaact
     3421 ctttattttt tgcttccgta atgccatcgg tataaaatac taaagtgtca ttctgtttca
     3481 gatcaatttt aatttcttct aaatggttac ggaatagagt atcatttgct agccctaacc
     3541 caattccaga aggtttaaat gtttcaattg aattatttcg gaccaataat gcgggacagt
     3601 ggcctgctct tgaaaagctc aggacattat tatcgaaatc aataattcca tacaaagcgc
     3661 ttacaaaatt ctttttattt aatgttgatt ctaaaatttc attagccttg attaaaatat
     3721 cctttggatt ttctatcatt cgagacaagg agctgaaaat tcctttcact tcagccatta
     3781 taaatgcagc agaaattcct ttacctgaaa catctgcaat aataaacccc aatttgtttt
     3841 ttgatatttc aaaaaaatca tagtagtctc ccccaacctc aaaagcagga atgaaaacag
     3901 aagtaattga aagatcttca aatacaggat cttttgcagg caatattttc ttttgaatat
     3961 cacgtgcaac atccaattct tttgcaagtc tttctttttc gatcgactgc tctagcaaca
     4021 aagaattttc tattgcaacc gatgcataat cagaaaaagt attaatagct gttttatctt
     4081 cctcataaaa tatcaattcg ttttttctgg cggcgattaa atatccttta atttcattat
     4141 agcttcttaa tggagatact gcaatgcttc caaatttttc cgaaagctga gatttattta
     4201 taaacttttg aagactgcaa atttttgtct cagtaatatt ctcacaaatc ccgctttcaa
     4261 gtaaaaactg atttattaaa tcagcatcga ccagagcaat atttttattt gccagaattt
     4321 ttctatcact attttctttc agaataatcc aggccgcatc tgcgctgctg acttttgttg
     4381 taatttcagt aatagtctca gcaagatctt caatatctaa aacctgtgtt ataagcttac
     4441 taaaatactg taatgatgaa acttcatttg cttttctatc ataggcctct gccgttggga
     4501 tgtggaataa ggtagtaaaa aacagcacag caaaataaat tcctccataa agcagtataa
     4561 gattgaaaaa ctgaaaagtg gtcggcgaaa agctcatcaa aattgtacca ttaaaatctg
     4621 ttttaccgct gttaatcaga tttattatga aaagtgctga aattataata gaaaggataa
     4681 gcagtgaaat tttttctttc ttatttatga atgctatcca cgaaattttt aatgaattaa
     4741 atgagataag aattattgag attattaaaa atgtattgta tatgaattca tattctgtgc
     4801 ttctgaaaag taaatttgaa actgcagata tagcaataaa tactgacatt gttataaagt
     4861 aagtgctgct tcttcgatac tgtttgtaaa agtacaattc ctttagagat gcaaaccagt
     4921 aacttgcaat acctaaaaac attaaaccgt aaaaagtatt aacaagattt tcaaatattc
     4981 cctgattaac cggtttatca ctgagttttt ggatttgaac attaagaata ttttccgcaa
     5041 acattatcac taaaaaaata aatgcacaga taataccaat gtttaaaacc agggctgaag
     5101 gatgcggatt ttttctggcc aataatttca taagatataa agtcagaacg attattagca
     5161 ttaaggaaag aagatcattt atcagtaaga tgagatatga attgatggga aaaaagaata
     5221 atcggtaaat aattatactg ccaaccaggg atgcaataat ccacaaaatc aggttaacgt
     5281 taatttttat actgctcaag ttcaaaataa tccgatttat gttaattgta tgggtttcgg
     5341 ccattaaaat aaatcaaatt tatgatcatt taaagaagag tattatgaat ggataatggt
     5401 ttgaaatgcg gtaaaataaa tggacaggcg gctcttaata ttttatattg agtgtgagtt
     5461 ttgagaaaat tatctaaccg ggatcatatc tcaaaaatat cttcatcgtt tgaggtatct
     5521 tttttaatag caccatcatt atttttcatc tgtggatctt tgatttcctc cctgaatttc
     5581 tgcagttctt tcctcaattt atcca
//
LOCUS       DASWBY010000063         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_2572, whole genome shotgun sequence.
ACCESSION   DASWBY010000063 DASWBY010000000
VERSION     DASWBY010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VF870_01360"
     CDS             complement(<1..433)
                     /locus_tag="VF870_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250853.1"
                     /translation="MKTFMAFWFLTISFFLYGQGTVKVTFDTGDKDINTHLSEVNAYA
                     ISNMDLFRKDLNEIFGATKEDLDRYLVKERRQPADVYYGYNLSKSTGKSFPTIMKMYT
                     DKKGWGSIAKDLGIKPGSDQFHALKDNTLKKIDKDKMNKAKK"
     gene            complement(525..863)
                     /locus_tag="VF870_01365"
     CDS             complement(525..863)
                     /locus_tag="VF870_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250854.1"
                     /translation="MKNVFKLLSILFLIITFVACSDDPPSFRVKNERATKANVQIKTV
                     ANTENINDVQPSTVTNYKEVAEGKVDVSAEIQNETDEPTNTFNAEKNNKYTIVIANTT
                     PPTIRIDVTD"
     gene            1088..2245
                     /locus_tag="VF870_01370"
     CDS             1088..2245
                     /locus_tag="VF870_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX9250855.1"
                     /translation="MHRRDFVKLGSIIAAAATPFNLFAADKFVKQYDNSSRQIDFIYD
                     GLSFSPKEYSDLLMKLADEGKIKPDYYSNGGVVEELENKFAKLLGKESAVFMPTGTMA
                     NHIAVRHLANTNRRIIVQEQSHFYNDSGDCAQTLSGLNLIPIGEDSVEFSLNEIEDII
                     SKTKTGRVETKVGVILIETPVRRQHDRMFMYDNQKVITNYAKNNEIKTHLDGARLFVQ
                     SVHTNLEPARYGELFDTVYTSLYKCFNSASGAILAGSKTFTTNLFHERRMFGGGLPSV
                     WPFAAVALHFADSFLDDYKTALQNAEKFISLIQKEDKFNVIKFENGSHIIRLNIKNAN
                     LKKFSESLSKKDIHLNAPDENGFLLKVNPSMNRETPQYLADSFIESLKDSE"
     gene            2381..2959
                     /locus_tag="VF870_01375"
     CDS             2381..2959
                     /locus_tag="VF870_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9250856.1"
                     /translation="MFSSLKKHINSRVLLTEEEFDICAKYFVSKKLKKHQFLLNEGDV
                     CRYIGFVNSGCLREYKIDNKGTEHILQFAIEDWWVSDLHSFLSGLPATYNIDALQDSE
                     VLLLERSAREELLDKCPKMERFFRLLIEASNVAAQQRVADSLSTSAEERYLKFIKTYP
                     QLFEQVPQNQIASYLGITPQSLSRIRKELSQK"
     gene            3114..>3621
                     /locus_tag="VF870_01380"
     CDS             3114..>3621
                     /locus_tag="VF870_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9250857.1"
                     /translation="MTTWKIDAAHSEVNFKVKHMLISTVRGNFDNFDATIETNKEDFS
                     DAVIKFEADVNTINTNNERRDTHLKSADFFDAENNQKMVFESTSVKKTSDFEMQVKGN
                     LTLRGVTKEVTLDVIYNGIVSGFGDSTIAGFEVRGKINRFDYGLKWNALTEAGGVVVS
                     NEVKIEILA"
BASE COUNT         1233 a          576 c          622 g         1190 t
ORIGIN      
        1 cctttttcgc tttattcatt ttatctttat caatcttctt caaagtgtta tccttaagtg
       61 cgtgaaattg gtcagaaccc ggcttaatcc caagatcctt tgcaatgctg ccccagccct
      121 ttttatctgt gtacattttc ataattgtcg gaaatgattt gccagtagat tttgaaagat
      181 tatatccata ataaacatcc gcaggctgtc ttctttcctt gaccaggtat cggtctaaat
      241 cttcttttgt tgctccaaat atttcattca aatcttttcg gaataaatcc atattagata
      301 ttgcatatgc attaacctct gagagatgag tgttaatgtc cttatcacct gtatcaaatg
      361 ttactttaac tgttccctga ccatagagaa aaaatgagat tgttaaaaac caaaaagcca
      421 taaatgtttt catatcataa cctttaacta atattataat tgtttattta aaattaagaa
      481 aggcggtaag taaaaaccta ccgcctttta atctaattaa ttatttaatc ggtaacatca
      541 atccgtattg tgggcggtgt tgtgttagca attactatag tatatttatt attcttttct
      601 gcgttaaagg tattagtcgg ctcatcggtt tcattctgaa tttctgctga gacatcaacc
      661 ttaccttcag cgacttcttt ataatttgta actgtactag gctgaacatc attaatattt
      721 tctgtattgg ctactgtttt aatctgaaca ttagctttag tggctctttc gtttttaact
      781 ctaaaagatg gcggatcatc tgaacaagca acaaaagtga taattaaaaa tagaattgaa
      841 agcaacttaa aaacattctt catttagagc tcctctgatt gttaataaaa tattacgata
      901 cataatgtat cgagtaagag gaacaattaa gatgccatta ttaaagccct tataattaaa
      961 tatttgagga atgattttta atatttttga agtgacattc agatttaatt aaattatgtt
     1021 gcagttaaag atataattac atttgttcta aaattatttg ttaacataat atatcccccc
     1081 gatcactatg cacagaagag actttgtaaa actgggcagc attatagcag cggcagcaac
     1141 accatttaac ctttttgctg ctgataaatt tgtgaaacaa tatgacaact ctagcagaca
     1201 aatagatttt atttatgacg ggttatcgtt ttctcccaaa gagtactctg acttactaat
     1261 gaagttggct gatgaaggga aaattaaacc tgattactat tctaacggtg gtgttgttga
     1321 ggaattagaa aacaagtttg ccaaactatt ggggaaagaa tctgccgtat ttatgcctac
     1381 aggaactatg gcaaatcata ttgctgtacg tcatttggca aatacaaatc gcagaataat
     1441 agtacaggaa caaagtcatt tctataacga ttcaggtgat tgtgcccaaa cacttagcgg
     1501 actcaatcta attccaatag gtgaagattc ggtagagttt agcttaaatg aaattgagga
     1561 tattataagc aagacaaaaa ctggtcgggt cgaaacaaaa gtgggcgtaa ttttaattga
     1621 aactcctgtt cgaaggcagc acgatcgaat gtttatgtat gataatcaga aagtaataac
     1681 caactacgca aaaaataatg aaataaaaac acatctggat ggagccaggc tctttgtaca
     1741 atcagttcat acaaacctgg agcctgccag atatggagaa ttatttgata ccgtttatac
     1801 ttctctatat aaatgcttta actcagcttc tggtgcaata ctggctggct caaagacttt
     1861 tacaacaaat ttatttcacg aaagaagaat gtttggagga ggcttaccat ccgtgtggcc
     1921 atttgctgca gttgctttgc attttgcaga tagtttttta gatgattata aaacagcttt
     1981 acaaaatgct gaaaagttta tttctttaat acaaaaagaa gataaattta atgtcatcaa
     2041 atttgaaaac ggttctcata ttatcaggtt gaatattaag aatgctaatt tgaaaaaatt
     2101 ttctgagtct cttagtaaaa aagatattca tttaaatgca cctgatgaaa atggattttt
     2161 attaaaagtt aatccttcta tgaacagaga aactcctcaa tatttggccg actctttcat
     2221 tgaatcatta aaagattctg agtaaaagta taagttaatt tgtttggttg tgagtaatta
     2281 tattgctatt gaaagcaaga tataatctgg aatgatctta ataaatcagc tgacaccttt
     2341 tattatattt aagtatgata ttatttaaat acttataaaa atgttttctt cactaaaaaa
     2401 acatatcaac agtagagtgc ttcttacaga agaagaattt gatatctgtg ctaaatattt
     2461 tgtttctaag aagctgaaaa agcatcaatt tcttttaaac gaaggtgatg tgtgccggta
     2521 tattggattt gtaaattcag gctgcctgcg cgaatataaa attgataaca aggggacgga
     2581 gcatatatta cagtttgcaa ttgaggattg gtgggtttca gatttgcata gttttctttc
     2641 aggtttacct gcaacttata atattgatgc tttgcaggat tctgaagttc ttttactaga
     2701 aagatctgca agagaagaat tgcttgacaa atgcccgaaa atggagcgct tttttcgtct
     2761 gctaatcgag gctagcaacg tggccgcaca acaaagagtc gctgattcac ttagcacatc
     2821 tgcagaagaa agatatctta aatttattaa aacttatccc caactttttg aacaagtacc
     2881 gcaaaatcaa attgcatcat atctcggtat cacaccgcaa tcattaagta gaatccgaaa
     2941 agagctatca cagaaatagt ttttagtaat cttttttttc tttatccgat ttaaaaagtt
     3001 accctatgtt aacttccttt tttaccctat gtgaatgtgc cagtgaactt ttcgacttaa
     3061 gtttgggtta gtattaaaca attaactaca aacaaaaata aaggaacaaa acaatgacaa
     3121 catggaaaat cgacgcagca cattctgaag tcaactttaa agtaaaacat atgctgattt
     3181 caactgtaag aggaaacttt gataatttcg atgcaacaat cgaaacaaat aaagaagact
     3241 tcagcgatgc agtaattaaa tttgaggctg atgtaaacac catcaacaca aataacgaac
     3301 ggcgtgatac tcacttaaaa tctgctgatt tctttgatgc agaaaataat caaaaaatgg
     3361 tttttgaatc aacatctgtt aagaaaacat ctgattttga aatgcaggta aaaggaaact
     3421 taacccttcg cggagttacc aaagaagtta cgcttgacgt tatctacaat ggaattgttt
     3481 ccggatttgg cgatagtaca attgcgggat ttgaagtacg cggaaagata aaccgttttg
     3541 attatggtct taagtggaat gcattaaccg aagctggtgg agtagtagta agcaatgaag
     3601 taaaaattga aattcttgct g
//
LOCUS       DASWBY010000064         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_340913, whole genome shotgun sequence.
ACCESSION   DASWBY010000064 DASWBY010000000
VERSION     DASWBY010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340913"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /locus_tag="VF870_01385"
     CDS             complement(<1..1897)
                     /locus_tag="VF870_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250858.1"
                     /translation="MKALITAFLLLLFSSQIIYSTGGQRLLSEPDPDIYKDKIVFTYE
                     NDLWLVSSNGGTATRLTSFPGNEFSAKFSPDGKSIAFTAGYDGTYSVYVMPSEGGEPV
                     RLTYTPSTAQSIAWTPDGKRIVFRSYWENYISRDPNLYFVNKEGSAPEKLPLDRGTLC
                     SFNSDGSKILYNRKGNEEYYWKRYKGGQHTDIWMYDFKQNQFTAISDYVGKNAYPIWI
                     GNEMYFISDRTNGISNLYVQDLNTKSIKEITSYSDFDVMCPETDGESIVYIQDGYINV
                     YDIKSNQSKQISVTIPSDRWALRDRVINPKDYIHSINISNDGKNAVIESRGDVFIIST
                     ENGNTKNISNTPGTREMHPEISPDGKWIAFFSDKTGEYQLYMQKTEGGEWIQLTSVLD
                     KFVYHLKWSPDGKKILFGNKEFAIFYVDVDSKKLVEIDRSNQLKNDEFYWEESDYSWS
                     PDSKWITYSFTNFNRNNVIYVYSLDQKKKYALTDDFYDNLYPSFDANGKYLYYVSSRN
                     FDIHMDFYEDNHIIATPQQVMVVQLRGNETPPFEDPLNSSDEKKTDEQFKIEVDGITA
                     RTYPLPIDPGNYFYLKAGNGKVLWCSVEYFTEDEYEEIFKPGSKTKWKLNIFDMSSES
                     LVALDDKI"
     gene            complement(2086..>2294)
                     /locus_tag="VF870_01390"
     CDS             complement(2086..>2294)
                     /locus_tag="VF870_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9250859.1"
                     /translation="VPPIGILAAYAYYQQGYVDLKIAGLICLGFVVGGYIGAKFAVSL
                     PTYILQKIFAISLFLIALKMFFAK"
BASE COUNT          732 a          416 c          363 g          783 t
ORIGIN      
        1 tgatcttatc atcaagtgct accaacgatt ctgaagacat atcaaaaata ttaagtttcc
       61 attttgtttt ggatcccggt ttaaatatct cttcatactc atcttcagta aaatattcta
      121 cagagcacca aagcactttt ccattgcctg ccttaaggta gaaataattt ccgggatcga
      181 tgggaagggg ataagttctt gcagtgattc catcaacttc tattttgaac tgttcatcag
      241 tttttttctc atcgctgctg tttaaagggt cttcaaatgg tggagtttca ttaccgcgta
      301 actgaacaac cattacctgc tgtggggttg caatgatgtg attatcttca taaaaatcca
      361 tatgaatatc aaagttcctg ctggatacat agtaaagata tttcccgttg gcatcaaaag
      421 atgggtagag attatcataa aaatcatccg ttaaagcata cttttttttc tgatccaaac
      481 tataaacata aataacattg ttacgattga aatttgtaaa gctgtaagta atccatttgc
      541 tatcgggcga ccagctgtaa tcactttctt cccaataaaa ctcatcattt tttaattggt
      601 tcgatctgtc aatctcaaca agttttttgg aatctacatc aacataaaaa attgcaaact
      661 ctttgttgcc aaaaagtatt tttttgccat caggtgacca tttaagatga taaacaaatt
      721 tatcaagcac ggatgttaat tgaatccact ctccgccttc agttttttgc atatagagct
      781 gatactcacc ggttttatct gaaaagaatg caatccattt tccatcaggt gaaatctcag
      841 gatgcatttc tctagtgcca ggtgtattag agatattttt tgtgtttccg ttttcagttg
      901 aaattataaa aacatcaccg cgagattcaa taactgcatt ctttccgtca ttcgaaatat
      961 ttattgaatg aatataatct tttggattta taactctgtc tcttagtgcc catctgtcag
     1021 atgggattgt aacggaaatc tgttttgact gatttgattt tatatcataa acattgatat
     1081 atccatcctg tatataaacg atactttcac catctgtttc agggcacatt acatcaaagt
     1141 cagagtaact ggttatttct tttattgatt ttgtatttag atcctgcaca taaagattag
     1201 atattccatt agtgcgatct gatataaaat acatttcatt tccaatccat atcggataag
     1261 catttttacc tacataatca gaaatagctg taaactgatt ttgtttaaag tcatacatcc
     1321 agatatcagt atgttgtccg cccttatatc ttttccagta atactcttcg ttgccttttc
     1381 gattatacag tattttactt ccatcagaat taaagctgca gagcgttcct ctgtccagag
     1441 gtaatttttc aggggcagaa ccttccttat tcacaaaata taaatttgga tcacgtgata
     1501 tataattttc ccagtaggat cggaaaacaa ttcttttacc atcgggagtc caggcgattg
     1561 attgtgcggt acttggtgtg taagttaatc taaccggctc gccgccttcc gaaggcatta
     1621 cataaacgct gtatgtgcca tcatatcctg cagtaaatgc aatcgattta ccatcaggag
     1681 aaaattttgc agagaattcg ttgccgggaa aactcgttaa tcttgtggca gtacctccat
     1741 tggagcttac aagccacaaa tcattttcat aagtaaaaac aattttgtct ttatagatat
     1801 ccggatcagg ttcagacaat aatctttgcc cgcctgttga ataaattatt tgagacgaga
     1861 agagtagcag taaaaatgca gtgatcaatg ctttcatatt actttcctaa taaaattgat
     1921 atttaaaatt acataaaatt aaatagtgat tgaattgaat taagtttcaa aacggttgtt
     1981 aagattacta tatatcgatt caagatatct taagttcaaa atctcagtta tctatcctgg
     2041 aataacttag tagtacaatc cgaacaaata ataatctctc attgattact ttgcaaaaaa
     2101 catttttaaa gcaattagaa aaagagaaat agcaaatatt ttttgaagaa tatatgtagg
     2161 aagactaact gcaaattttg ccccaatata tccgccaaca acaaaaccta aacaaatgag
     2221 tcctgctatt tttagatcaa catatccttg ttgataataa gcgtatgcag caagtattcc
     2281 tattggcgga acca
//
LOCUS       DASWBY010000065         3365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_303359, whole genome shotgun sequence.
ACCESSION   DASWBY010000065 DASWBY010000000
VERSION     DASWBY010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303359"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /gene="gyrB"
                     /locus_tag="VF870_01395"
     CDS             <1..1833
                     /gene="gyrB"
                     /locus_tag="VF870_01395"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX9250860.1"
                     /translation="DNSIDEALGGYNDRVIVTIKKDGSVSVEDRGRGIPVDMHPVEKR
                     SALEVVMTVLHAGGKFDKDSYKVSGGLHGVGVSVVNALSEKMTAEVRRDNKIYFQEYH
                     KGIPVEKVKQIGTYKGDQTGTTITFKPDAEIFKTTKFNFETVAERLRELAYLNKDVTM
                     ILKDENDGQEETYRFKGGLIDFVKYLDEQRTAMHKPVYIEGEKENTPVEISFEYSDSY
                     SENVHSYVNNINTIEGGTHLVGFRTALTRTLNNYAYKNGLIKENNKITLTGDDFREGL
                     TAVISVKVMEPQFEGQTKTKLGNSEVKSIVETIVGEKLSEYLEENPSVGKKIIEKCMR
                     AAEAREASRKARDLVRRKNALDSMHLPGKLADCSINDPEHCEIYIVEGDSAGGSAKQG
                     RDRRFQAILPIKGKILNVEKAKLNKILENQEIQAMIAALGAGIGEEFNPEKSRYGKII
                     LMTDADVDGSHIRTLLLTFFYRHMKELITSGRVYIAQPPLYKVKKGKEEHYAFDDEER
                     DKVLKQFRKDMKTKAGVVDEDEEVDEEGQPKGVTISRYKGLGEMNPEQLWSTTMNPET
                     RTVLQVNVESAAAADKIFETLMGDAVEPRREFIEKHAKYANLDI"
     gene            1871..>3365
                     /locus_tag="VF870_01400"
     CDS             1871..>3365
                     /locus_tag="VF870_01400"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEX9250861.1"
                     /translation="MTTIFEKVVPRTLEDEMKSSYIDYAMSVIVARALPDVRDGLKPV
                     HRRVLFGMHELGLPHNRPYKKSARIVGEVLGKYHPHGDTAVYDSMVRMAQEWSLRYPL
                     VEGQGNFGSVDGDSPAAMRYTEARLARIADEMLRDLDKNTVDFANNFDESLQEPTVMP
                     SYLPNLLINGASGIAVGMATNIPPHNISEVIDGCVALIENPKATAADLMKYVKAPDFP
                     TGGIIYGYEGVKEALLTGRGRVVIRAKANVETLKNDRENIIITEIPYQVNKAVLIEKI
                     ADLVREGKITEISNIRDESDRDGMRVVIELKRDAQPAVVLNQLFKHTQMQVTFGVIML
                     ALVHGAPKVLNLQEMIQHFLDHRMEVLIRRTKYELDAAEKRAHILEGYIIALDNIDAV
                     IETIKKSKDTETAKNNLMKKFKLSEIQAKAILDMRLQRLTGLERKKIEDEYKETIKLI
                     ERLRGILDNERTRKKIIKDELLALKDKYGDERRTEIIKDYKEFALEDI"
BASE COUNT         1234 a          551 c          743 g          837 t
ORIGIN      
        1 gacaatagta ttgacgaagc attaggggga tataatgacc gcgttatagt aaccattaaa
       61 aaagatggaa gcgtgtcagt tgaagatcgc ggaagaggaa ttcctgttga tatgcatcct
      121 gttgaaaaac gttctgcatt agaagttgtt atgaccgttc ttcacgcggg aggaaagttt
      181 gataaggatt catataaggt ttcgggagga ttgcatggtg tgggtgtttc ggttgtaaat
      241 gctttatcag aaaaaatgac tgcagaagta cgaagagaca ataaaattta ttttcaggaa
      301 tatcacaaag gaattccagt cgaaaaagta aaacagatag gaacttataa gggcgaccaa
      361 acaggaacca caattacttt caaaccggat gcagaaattt ttaaaacaac aaaatttaat
      421 tttgaaactg tagcggaaag attgcgagaa ctggcgtatc tgaacaaaga cgttacaatg
      481 attcttaaag atgaaaacga tggtcaggaa gaaacataca gatttaaagg cggactaata
      541 gactttgtta aatatctgga cgaacaaaga accgcaatgc ataaacccgt ttacattgaa
      601 ggagaaaaag aaaacactcc tgttgaaata tcttttgagt acagcgattc ctattcagag
      661 aatgttcact cttatgtaaa caacatcaac acgatagaag gcggaacaca cttagttgga
      721 tttagaactg cacttaccag gacactaaac aactacgcat ataaaaacgg attgatcaaa
      781 gagaataata aaataaccct aaccggcgat gattttcgtg aaggccttac tgctgtaatt
      841 tcggtaaagg ttatggagcc acagtttgaa ggacaaacaa aaacaaaact gggtaacagc
      901 gaggtaaagt cgatagttga aactattgtt ggcgaaaaat tatcagagta tttagaagaa
      961 aatccttcag tcggtaaaaa gattattgaa aaatgtatgc gcgccgccga agcaagagaa
     1021 gcttcgcgca aagcacgaga ccttgtaaga agaaagaatg ctttagattc gatgcacctg
     1081 ccgggtaaac ttgcagattg ttcaattaat gatccagaac attgcgaaat ttatatagtt
     1141 gaaggcgact ctgcgggcgg atctgcaaag caagggcgcg atagaagatt ccaggcgata
     1201 cttccaatta aaggaaaaat tcttaatgta gaaaaagcaa agcttaataa aattttagaa
     1261 aaccaggaga tccaggcaat gatagctgct cttggcgctg gtattggcga ggagtttaat
     1321 cccgaaaagt cgcgttacgg aaaaattatt ttaatgacag atgcagacgt tgatggaagc
     1381 catattcgta cactactttt aacattcttc tacaggcaca tgaaagagct cataacatcg
     1441 ggaagagttt atattgcgca accgccgttg tataaagtta aaaaaggaaa agaagaacat
     1501 tatgcttttg atgatgaaga aagagataaa gttttaaagc aattcagaaa agacatgaaa
     1561 acaaaagccg gtgttgttga tgaagacgaa gaagttgatg aagaaggaca accaaaggga
     1621 gtcacaattt cccgttacaa aggattagga gaaatgaatc ccgaacaact ctggtcaaca
     1681 acaatgaatc ctgaaacaag aaccgtactg caggtaaatg ttgaaagcgc agcagctgca
     1741 gataaaatat ttgagacact tatgggtgac gcagttgaac caagaagaga atttatagaa
     1801 aagcatgcta agtatgctaa tcttgatata tagtttaaaa gtttatttat ttaagtaatt
     1861 atagaaatat atgaccacaa tatttgaaaa agtagtaccc agaacattag aagatgaaat
     1921 gaaatcatct tacatcgatt atgcaatgag cgttattgtt gcgcgtgcac tgcccgatgt
     1981 aagagatgga ttaaaacctg ttcaccgtag agttttattt ggtatgcacg aactgggttt
     2041 gccgcacaac agaccataca aaaagtcagc aagaatagtt ggagaggtgc tcggtaagta
     2101 tcacccgcac ggagatactg ctgtctacga ttcaatggtt cgtatggcgc aggaatggtc
     2161 tttgcgttat ccacttgttg aaggacaggg aaactttgga tcagttgatg gcgattcacc
     2221 cgcagcaatg cgttataccg aagcaagact tgcaagaatt gcagatgaaa tgcttcgcga
     2281 tcttgataaa aacactgttg attttgcaaa caactttgat gagtctttgc aagagccaac
     2341 cgttatgcct tcttatcttc caaatcttct aataaacggg gcaagcggaa tagcagtcgg
     2401 aatggcaaca aacattccac ctcataacat tagcgaagtt attgatggat gcgttgcttt
     2461 gattgaaaac ccaaaagcaa ctgcggctga tttgatgaaa tatgttaagg cacctgattt
     2521 tccaacaggc ggaatcatct atggatatga aggagttaaa gaagctttgc ttacgggaag
     2581 gggccgcgtt gttatccggg caaaggcaaa cgttgagacc ctgaagaacg atagagaaaa
     2641 tattattatt actgaaattc cttatcaggt aaataaggcg gtgctaattg aaaaaattgc
     2701 tgaccttgtt cgtgagggaa agataacaga aatatcaaac ataagagacg aatctgaccg
     2761 cgacggtatg cgcgttgtga ttgaacttaa acgtgacgca cagccggcag ttgttctcaa
     2821 tcaattattt aagcacaccc aaatgcaggt tacgtttggc gtaattatgc tggcacttgt
     2881 tcatggagca ccaaaagtac ttaatcttca ggaaatgatt caacacttct tagatcatag
     2941 aatggaagtg cttatacgca gaaccaaata tgagctggac gctgcagaga aacgcgcaca
     3001 cattttagag ggctatataa ttgctcttga taatattgac gcggtaattg aaacaattaa
     3061 aaaatccaaa gacacagaaa cagctaaaaa taacttgatg aaaaaattca agttaagcga
     3121 gatacaagca aaagcaattt tggatatgag attgcagcgc ttaaccggat tagagcgcaa
     3181 aaaaatagaa gacgaataca aagaaactat taaactgatc gaaagactta gaggaattct
     3241 ggataacgaa agaacaagaa agaaaattat taaagacgaa cttcttgcac ttaaagataa
     3301 atatggtgat gagcgcagaa cagagatcat aaaagattat aaagaatttg ccctagaaga
     3361 tatta
//
LOCUS       DASWBY010000066         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_454606, whole genome shotgun sequence.
ACCESSION   DASWBY010000066 DASWBY010000000
VERSION     DASWBY010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454606"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VF870_01405"
     CDS             complement(<1..332)
                     /locus_tag="VF870_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HEX9250862.1"
                     /translation="MIGSIFLSTALVFSIIAMVMYYLSFKGYKNTLNIARYSYHGMAI
                     FVIAASTFLWYAILTHQYQYKYIFSYSNNSLSTGLLFSSFWGGQEGSFMLWLLLTSII
                     GVILQSYSS"
     gene            complement(462..851)
                     /locus_tag="VF870_01410"
     CDS             complement(462..851)
                     /locus_tag="VF870_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX9250863.1"
                     /translation="MKNKYIFGGFIIVLFLGIMAYLFTQSNIKYESDFANVKASDKTV
                     KATGSWVKEKNYDMNREQNTFSFFMKDQSGNEMKVIYEGAMPNNFESATSVVVTGSYR
                     NGYFHAKDILTKCPSKYEEQKVQSSSL"
     gene            complement(841..990)
                     /locus_tag="VF870_01415"
     CDS             complement(841..990)
                     /locus_tag="VF870_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HEX9250864.1"
                     /translation="MEQFLSQNAIYIVMIIVLIVWAGIFGYMFSLDKRIKKAEKELLG
                     EKNEK"
     gene            complement(992..1696)
                     /locus_tag="VF870_01420"
     CDS             complement(992..1696)
                     /locus_tag="VF870_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEX9250865.1"
                     /translation="MIWKIFLFLLLAFVSIAGIAFPIVEKPSAWYEFPLIPGLEENAK
                     IIFFHVPTAWLTVVAFFMSTIYSFKYLRYKNLDDDVKSYAAAQLGMIFCILATVTGAV
                     WAKFAWGSYWSWDPRQTSIFALLLIYGAWFALRSSLESEEKRATLSSVYSMIAFVTVP
                     FFIFIMPRIMTGLHPGSADDSNAGPVVDFKMNGNMQLIFFLSLIGFTILYFWMWNVGY
                     RSILLKEKLDKKLIRG"
     gene            complement(1729..2394)
                     /locus_tag="VF870_01425"
     CDS             complement(1729..2394)
                     /locus_tag="VF870_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX9250866.1"
                     /translation="MNSKSYNLFLKDFKSELRTRYAINALAMFIIVAISVILFSVGNE
                     KISTSLTGGLFWVVIFFTAMSGLSRVFVSEEERGTALTLQLIAAPSTVFTGKLIFNIA
                     LVFTMNTIIAVLYSAIFEAFIIKNFGLFLASFVLGNIGLAAASTIIAAIIAKAGAKGT
                     LYPVLSFPILLPLILTSVQLTLFSLDGISFERAKFELAIVVSYDIIMLTASYMLFDFI
                     WKE"
     gene            complement(2395..>2889)
                     /locus_tag="VF870_01430"
     CDS             complement(2395..>2889)
                     /locus_tag="VF870_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9250867.1"
                     /translation="LVKIIAGIIGASKGKIVHKLSDKEISEEHLHDHIGFVSPYLVLY
                     EEFSTYENLILFSDIRGISFNKERVDYLLNKFLLYKRKDDLLKTYSSGMKQRVKFIFA
                     LMHSPELLILDEPTSNLDDEGKNSVYELVKEEAQKNIVLIASNEKHDLELCGEIVYLE
                     KYKN"
BASE COUNT          998 a          512 c          440 g          939 t
ORIGIN      
        1 gatgagtatg attgaagtat aactccgatt attgaggtaa gaagaagcca taacataaag
       61 cttccctcct gcccgcccca aaaagatgaa aataataatc cggttgataa tgaattattg
      121 ctgtaactaa aaatatattt gtattgatac tgatgtgtta aaattgcgta ccacaaaaaa
      181 gtggaagcag caattacaaa aatagccatt ccgtgataac tatagcgtgc aatgtttaat
      241 gtattcttat aacccttaaa ggaaaggtaa tacattacca ttgcaattat actgaaaaca
      301 agagcggtac ttaaaaaaat acttccgatc atattacctc gctaagcgca ttgtgcctgg
      361 tgcaaagagc agtgcacgat tacaatgctt tttattttaa ttagtttcta aaagaaaaac
      421 tttgaaaagg cgctatgctc tttgcgcctt gctctttgga cttataaact tgaagactga
      481 accttttgtt cttcatactt gcttggacat tttgttaaaa tatctttagc gtggaaatac
      541 ccgttgcggt atgaaccggt tacaactaca cttgtagcac tttcaaaatt atttggcatt
      601 gcgccttcgt aaataacttt catttcattt cccgattggt ctttcataaa gaacgagaaa
      661 gtattttgtt cacgattcat atcataattt ttttctttaa cccagcttcc agttgcttta
      721 acagttttat ctgaagcttt aacatttgca aagtcactct catacttgat gttactttgt
      781 gtaaacagat atgccattat tcccagaaac agaacgatga taaatccacc gaagatatat
      841 ttatttttca tttttttctc ctaataattc tttttcagct tttttaattc ttttatcaag
      901 gctaaacata tatccaaaaa ttcctgccca aactatcagg acgattatca tgacaatgta
      961 aatagcattt tgtgatagaa attgttccaa cttaacctct gattaatttt ttatctaatt
     1021 tttcttttaa taagattgat ctgtaaccga cattccacat ccagaagtaa aggattgtaa
     1081 atccaattaa agatagaaag aatatcaact gcatatttcc attcatttta aaatctacta
     1141 ccggacctgc attagaatca tctgctgaac cgggatgaag tcctgtcata attcttggca
     1201 taataaaaat aaaaaacgga actgttacaa aagcaatcat agaataaaca gaagataatg
     1261 tagctcgttt ttcttcagat tctaaagatg aacgaagtgc aaaccaggca ccgtaaatta
     1321 ataataatgc aaatatactt gtttgtctgg gatcccaact ccaatatgat ccccaggcaa
     1381 actttgccca aactgcgccc gttacggttg caagaatgca gaagatcatt ccaagctggg
     1441 ctgcggcata agattttaca tcatcatcca aatttttata tcgtaaatac ttgaagctat
     1501 aaattgttga cataaaaaat gctacaactg taagccaggc cgttggaacg tggaagaaaa
     1561 taatttttgc gttttcttca agtcccggaa ttaaagggaa ctcataccag gcacttggtt
     1621 tctctacgat gggaaatgcg attccagcaa tagagacaaa ggccagtaat aaaaataaaa
     1681 atattttcca gatcataatt ttttttataa tagtttagtt aaaaaatgct attctttcca
     1741 aataaaatca aacaacatat aagaagcggt caacataatt atatcgtaac tcacaacaat
     1801 tgccaattca aacttagctc tttcaaagga tatgccatct aatgagaaca atgttaattg
     1861 aacagaagtt aaaatcaaag gcaataaaat gggaaatgat aacacaggat acaaggttcc
     1921 tttagcaccg gcttttgcga ttatggcggc aattattgtt gaggctgctg ccaaaccaat
     1981 attacccagt acaaaagagg ctaaaaaaag tccgaaattt tttattataa aagcctcgaa
     2041 tattgctgaa tagagtacag caataattgt gttcatagta aagaccagcg caatgttaaa
     2101 tatcaattta cctgtaaaaa ctgttgatgg tgcggcaatt aattgtaatg ttaatgctgt
     2161 gccgcgttct tcctcagaaa caaaaactct tgataaacct gacattgctg taaaaaatat
     2221 tacaacccaa aataatcccc cggttaaact tgtacttatt ttttcattcc ctactgaaaa
     2281 taaaatgaca ctaattgcaa caataatgaa cattgcaaga gcattaattg cataccgggt
     2341 acgcaactca gatttaaagt cttttagaaa taagttgtaa gattttgagt tcatttaatt
     2401 tttatatttt tccagataaa caatttcgcc gcacaattct aaatcatgtt tttcgtttga
     2461 agcaattaat acaatgtttt tttgggcttc ttctttcact aattcataaa ctgaattttt
     2521 tccttcgtca tcaaggtttg atgttggttc atcaaggatt aataattcag gcgagtgcat
     2581 taatgcaaag ataaatttaa ctctttgttt cattcctgat gaatatgttt tcagcaaatc
     2641 atcttttctc ttatacaata aaaatttgtt aagaagataa tcaacccgtt ctttgttaaa
     2701 tgatattcct ctaatatctg aaaacagaat aaggttttca taagtcgaaa actcttcata
     2761 taaaacaaga taaggcgaaa caaatccgat gtgatcgtgt aaatgctcct cagaaatttc
     2821 tttatcacta agcttgtgaa cgattttgcc ttttgaggca ccaattattc ctgcaataat
     2881 ttttactag
//
LOCUS       DASWBY010000067         6513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_153063, whole genome shotgun sequence.
ACCESSION   DASWBY010000067 DASWBY010000000
VERSION     DASWBY010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153063"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1062
                     /gene="lpxK"
                     /locus_tag="VF870_01435"
     CDS             10..1062
                     /gene="lpxK"
                     /locus_tag="VF870_01435"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX9250868.1"
                     /translation="MKFLKILLAPFVFLYGSIINVRNLFFDKAVFKSEKVNAKVISVG
                     NINVGGSGKTPLVIYVTNLLKNSGYKVGVLSRGYGRKSSGYKLVSKGDTILTSVEECG
                     DEIYHTALECKVAAAVSENRVKGANKLIEETKIDSIVLDDAFQHRWIDRDIDLVIIDQ
                     SFINNKSFFTHNLLPAGDLREGFESLKRADAIIINRKFLDKEEINSEMKNHFDGRNIF
                     TSYYKAISFVDAMRKIEYSLDDFKGQESLVVSGIANPQSFLNVLGKVHVNTQNKMIFR
                     DHKNYTSKEVQQIRKQFYTTNSHSVVTTEKDAVKLSRFNREFDDVDIFYLKINLCMDD
                     EESFNKYLINKLKSAS"
     gene            1108..3918
                     /gene="ppdK"
                     /locus_tag="VF870_01440"
     CDS             1108..3918
                     /gene="ppdK"
                     /locus_tag="VF870_01440"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX9250869.1"
                     /translation="MAQTKYVYYFGGKKAEGKAEMKALLGGKGANLAEMVNIGLPVPA
                     GFTITTEVCTAYYKNKKKYPKELEKQVKDALAKVEKQMGAKFGDLENPLLLSIRSGAR
                     ASMPGMMETILNAGLNNQTREALIKKTGNPRFVYDSHRRLIQMYSDVVMEKAAGIEPE
                     EGKGVRQQLEKELHKMKEARGAHADTDLTADDLKELIEIYKAKVQEVLGKPFPEDPME
                     QLWGAVSAVFQSWMGKRAISYRRIEGIPDDWGTAVNVQSMVFGNMGDSSATGVAFTRN
                     PATGENYFYGEWLTNAQGEDVVAGIRTPNPINEVGKSEHTEHLESLETAMPQTYKQLH
                     NIQRSLERHYHDMLDIEFTIQDGTLYMLQCRVGKRNGPAAVKMALDMYKEKIISKEEA
                     VTRVQPSQLDELLHPIIDPAVEIKTKAVAKGLPAGPGGAAGQVVFSANDAVAWAKQGK
                     KVILVREETNPEDIEGMRAAQAILTARGGMTSHAALVARGWGKCCIVGAGSIKMNYDE
                     KYFTVGDVTIKEGDWLTLNGSKGTVYLGELPMIKAAEENPDFQAFMKLCDDVRKLKVR
                     TNADTPEDAARARAFGAEGIGLFRTEHMFYGKNSEEPLFKLRKMIHSKTEAERISALN
                     ELFPYVKKDVKETMSAMDGYPVTFRTLDPPLHEFVPQRVEEREKLALSLGISLEELNN
                     RADALHENNPMMGHRGVRLGITYPEITEMQVRAIFEATAELLKEGKKPFPEIMIPVTC
                     HVNEINHQYEIVTRVHAEVCAKFGLKKIAHLTGTMIEIPRACLTADKIAETAQFFSFG
                     TNDLTQMGFGFSRDDIGGFLPEYLEKKILPEDPFQSIDQDAIGKLMEIAVKGGRGTRP
                     DLKIGICGEHGGEPKSVEFCHKIGLNYVSCSPFRVPIARLAAAQAAVKDMKNTKPAKA
                     SSKKTAAAKKTVVVKKKVSKKK"
     gene            4070..5104
                     /gene="queA"
                     /locus_tag="VF870_01445"
     CDS             4070..5104
                     /gene="queA"
                     /locus_tag="VF870_01445"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX9250870.1"
                     /translation="MKLSDFKYNLPKTSIAKYPVSPRDKSKLLVVHKDTGELEDKKFT
                     DVIDYMEKGDVLVVNETRVFQARLFGKKEKTQAKIEVFLLRELNKEDCIWDVIVDPAR
                     KVRIGNKIYFDNNLWCEVIDNTTSRGRTVRFNQPGNVFHAVEKIGLTPLPPYIKREPE
                     PKDKENYQTVYAKVDGAVAAPTAGLHFTTKLLEKIEKKGIHIVPVVLHIGLGTFRPVE
                     VEDLTKHKMDSEYYEVSAHTAEVVNKAKHAKHSVIVVGTSACRALETCTTADGFLRQG
                     RGWTDKFIYPPHVFHITEKLITNFHAPESTLLMLTSALGDTELIMKAYKKALKDKYRF
                     LSYGDAMMIV"
     gene            5101..5775
                     /gene="ispD"
                     /locus_tag="VF870_01450"
     CDS             5101..5775
                     /gene="ispD"
                     /locus_tag="VF870_01450"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX9250871.1"
                     /translation="MMTIAIIPAGGKGLRSGSATPKQYLKVKSKEIIVYTLQIFQKNK
                     SIDKIIIAAEPDYFNLLIKLIKEYKLSKVKLIVEGGKTRQDSVYNAALSSGADDNDLL
                     VVHDAARALLPENTLTNAIETAKQKGSALVCIKAKDTLIKGKKFVDGYLERDEVYYVQ
                     TPQIFKYKDLYRALIKAEKENFIGTDESMLVKRIGKKVNIVEGSVFNFKITTSEDVEL
                     FKKLVK"
     gene            complement(5777..6244)
                     /gene="ybaK"
                     /locus_tag="VF870_01455"
     CDS             complement(5777..6244)
                     /gene="ybaK"
                     /locus_tag="VF870_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEX9250872.1"
                     /translation="MNKTNAIRILESHKIKYETVSYEVDESDLSGDTVALKISADPET
                     VFKTLVCIGDKTGHIVFCIPVTQELNLKKAAAASGNKKVEMIKLKDLLPLTGYIRGGC
                     SPVGMKKLFPTYLDETAQIFESIFVSAGVRGTQMKLSPDKLLRIIGGNYADLI"
     gene            complement(6231..>6513)
                     /locus_tag="VF870_01460"
     CDS             complement(6231..>6513)
                     /locus_tag="VF870_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250873.1"
                     /translation="QDVEAQLGILNLIKKLKEEFNLTIICISHDLNILKHLSDEIVIM
                     YNGSIVESGQTKTVIDNPTNDYTKFLLSAQSLNLTEADIHTFHNTYEQN"
BASE COUNT         2277 a         1011 c         1310 g         1915 t
ORIGIN      
        1 gttgattaaa tgaaattttt aaagatttta ttagcaccat ttgtgttttt atatggatcg
       61 ataattaacg ttagaaattt gttctttgat aaagctgttt ttaaatctga aaaagttaat
      121 gctaaagtaa tttccgttgg aaatataaat gttggaggat caggtaaaac tccacttgta
      181 atttatgtta ctaatctttt aaagaactca ggttacaaag ttggtgtttt aagtcgtgga
      241 tatggaagaa aatccagcgg ttacaaactt gtatccaaag gcgatacgat tttaacttca
      301 gtcgaagaat gtggcgatga aatttatcac actgctttgg aatgtaaagt tgctgcagct
      361 gtttctgaaa atcgtgttaa aggtgccaat aaattaattg aagaaacaaa aatagattca
      421 atagttttgg atgatgcttt tcagcatcgc tggattgaca gggatattga tctggttatt
      481 attgatcaga gttttataaa taataaaagt ttttttacgc ataatctgct acctgcaggc
      541 gatttgaggg aaggatttga atctttaaaa agagctgatg caataattat taacagaaaa
      601 tttctcgata aagaagaaat aaattctgaa atgaaaaatc attttgatgg cagaaacatt
      661 tttacatcct attataaggc gataagtttt gtagatgcaa tgcgaaagat cgaatacagc
      721 cttgatgatt ttaagggaca ggaaagttta gtggtctcgg gaatagccaa tccacaatca
      781 ttcttaaatg tactgggtaa agttcatgtt aatacgcaga ataaaatgat atttagagat
      841 cacaagaact atacatctaa agaagttcag cagatacgta agcaatttta tacaactaat
      901 tctcattcgg ttgttacaac tgaaaaagat gcagttaagc tttcaagatt taacagagaa
      961 tttgatgacg tagatatttt ctatttaaaa ataaacttgt gtatggatga tgaagaatca
     1021 ttcaacaagt atttaattaa taaactaaaa agcgcttcgt aaaaagataa tttaattaga
     1081 taatcttata acggaggaaa ggtaattatg gctcaaacaa aatatgttta ctacttcggt
     1141 ggtaaaaaag cagaaggtaa agctgagatg aaggctttac ttggaggtaa aggtgcaaac
     1201 cttgcagaaa tggttaacat tggacttcct gtacctgcag gatttacaat tacaacagaa
     1261 gtgtgtacag catactacaa gaacaagaaa aaatatccca aagaacttga aaaacaagtt
     1321 aaagacgctc ttgctaaagt tgaaaaacaa atgggtgcta agtttggcga tttagaaaac
     1381 ccgctcttac tttcaattcg ttcaggtgct cgcgcttcca tgccgggtat gatggaaact
     1441 attcttaatg ctggattaaa caatcaaacg cgtgaagcct tgattaaaaa aacaggtaat
     1501 ccaagatttg tttatgattc acatcgccgc ttaatacaga tgtacagcga cgtggttatg
     1561 gaaaaagcag caggcatcga accggaagaa ggtaaaggcg ttagacagca attggaaaaa
     1621 gaactccaca agatgaaaga agcccgcgga gcgcacgctg acacagacct gactgcagat
     1681 gatttaaaag aattgattga aatctacaaa gcaaaagttc aggaagtttt aggaaaacct
     1741 tttccggaag atccaatgga acaactttgg ggcgctgtat cagcagtatt ccagagctgg
     1801 atgggtaaac gtgcaatttc ttacagaaga attgaaggaa tccctgatga ctggggaaca
     1861 gctgtaaatg ttcaatcaat ggtttttgga aatatgggtg atagttctgc aactggtgta
     1921 gcattcacgc gtaatcctgc gaccggtgaa aattattttt acggtgaatg gttaaccaat
     1981 gcacaaggtg aagatgttgt tgccggaatc agaacaccga atccaattaa cgaagttgga
     2041 aagagtgaac atactgaaca tcttgaatct cttgaaacag ctatgccgca gacttacaag
     2101 caattgcata acatacaaag atcgcttgaa agacattatc atgatatgct tgatattgaa
     2161 tttacaattc aggatggaac actttatatg ctgcaatgcc gagtaggaaa aagaaacgga
     2221 ccggctgctg taaaaatggc gcttgatatg tacaaagaaa aaattatctc aaaagaagaa
     2281 gctgtaacac gcgttcagcc ttctcagctt gatgaattat tacatccaat tattgatcct
     2341 gctgttgaaa taaaaacaaa agcagttgct aaaggattac ctgcaggacc aggcggtgcc
     2401 gccggacaag ttgtattttc cgccaatgat gctgttgcat gggctaagca aggtaaaaaa
     2461 gtaattcttg ttcgtgaaga aacgaatcct gaagatattg aaggtatgcg tgctgcacaa
     2521 gcaattttaa ctgcccgtgg tggaatgaca tcacacgctg cacttgttgc tcgcggttgg
     2581 ggtaaatgct gtatcgttgg tgccggatca atcaaaatga attatgatga aaaatatttt
     2641 accgttggtg atgttacaat taaagaaggt gattggttaa cacttaacgg ttcaaaagga
     2701 actgtttact taggtgaatt gccaatgatt aaagctgcgg aagaaaatcc tgacttccag
     2761 gcatttatga aattgtgtga tgatgttcga aaattaaagg taagaactaa tgctgatact
     2821 cctgaagatg cagcacgtgc aagagctttt ggtgcagaag gtatcggact tttcagaaca
     2881 gaacacatgt tttacggaaa aaattctgaa gaaccattat tcaaacttcg taagatgatt
     2941 cattcaaaaa ctgaagccga aagaatatca gcgttaaatg aattattccc ttacgttaaa
     3001 aaagatgtta aagaaacaat gtcagcaatg gatggatatc ctgtaacatt cagaacactt
     3061 gatccgccgt tacatgaatt tgttcctcaa agagttgaag aaagagaaaa acttgcttta
     3121 agtcttggaa tatctcttga agagttgaat aaccgtgctg atgcattaca tgaaaacaac
     3181 ccaatgatgg gacatcgtgg tgtaagatta ggaattactt atccagaaat tactgaaatg
     3241 caggttcgag ctatctttga agcaaccgcg gagttattaa aagaaggaaa gaaacctttt
     3301 ccggaaataa tgattccggt tacctgtcac gtaaatgaaa tcaatcatca gtatgaaatc
     3361 gtaacgagag ttcatgcaga agtttgtgca aaatttggat tgaagaaaat tgcacactta
     3421 accggaacta tgattgaaat tccacgtgct tgtttaactg cagataagat cgctgagact
     3481 gcacaattct tctcattcgg cacaaacgat cttacacaaa tgggatttgg attttcacgt
     3541 gacgatatag gtggattttt accagaatat cttgaaaaga aaatccttcc tgaagatcca
     3601 tttcaatcaa tagatcagga tgcaattggt aagctgatgg aaattgcagt taaaggcgga
     3661 cgcggtacaa gacctgattt aaaaattggt atctgcggag aacatggcgg tgaaccaaaa
     3721 tcagttgaat tctgtcataa gatagggttg aattatgtaa gttgttctcc attccgtgtt
     3781 ccgattgcaa gattagctgc tgcacaggct gctgttaaag atatgaagaa tacaaaacct
     3841 gctaaagctt catcaaaaaa aacagctgca gctaaaaaga cagtagtagt aaagaaaaaa
     3901 gtatctaaga aaaaatagta agtaaatgag gttgtcttaa agatgtcatt ccgaacttgt
     3961 ttcggaatct aaaatatacg aattcttgaa gaagctgaaa taaattcagc ttgactttaa
     4021 aatttttaag acaaccgata attaaaataa taagagaagg tagagataaa tgaagctatc
     4081 agatttcaag tataacttgc caaaaacttc catcgcaaag tatccggtta gtccgcgcga
     4141 taaatctaaa ttgttagttg tccataaaga taccggcgag ttggaagata aaaagtttac
     4201 tgatgtaatt gattatatgg aaaaaggtga tgtacttgtt gttaatgaaa caagagtatt
     4261 tcaggcacga ttgtttggaa agaaagaaaa aacccaagct aagattgaag tttttctttt
     4321 gagagaattg aataaagaag attgcatctg ggatgtaatt gttgatcctg caagaaaggt
     4381 tagaatcggc aacaagattt attttgataa taatctttgg tgcgaagtta ttgataatac
     4441 aacttccaga ggtagaacgg taagattcaa tcagccgggt aatgtatttc acgctgttga
     4501 aaaaatcggg ttaaccccgc ttccgcctta tattaagcgt gagccggaac caaaggacaa
     4561 ggaaaattat caaacagttt atgctaaagt tgatggtgca gtagccgccc caacagcggg
     4621 attgcatttt acaaccaaac tgcttgaaaa aattgagaaa aaaggaattc atattgttcc
     4681 tgttgttctt cacatcggcc tgggaacttt tagaccggta gaagttgaag atctaaccaa
     4741 acataaaatg gattctgagt attacgaagt atcagcacat actgcggaag ttgtaaacaa
     4801 ggcaaagcac gctaaacaca gcgttattgt tgttggtaca agtgcttgtc gtgctttaga
     4861 aacatgtaca acagcagatg gtttcttaag acaaggcaga ggctggacag ataaatttat
     4921 ttatcctcct cacgtttttc atattacaga aaagcttatt acaaactttc atgcaccgga
     4981 atcaacttta cttatgctta caagtgcatt gggtgatact gagttgataa tgaaagcata
     5041 caagaaagca ttaaaagata agtatcgctt tttaagttac ggcgatgcta tgatgattgt
     5101 atgatgacaa ttgcaattat tcctgctggt ggaaaaggtt tacggagtgg ctcggccact
     5161 cctaaacaat atctaaaagt aaaaagcaaa gaaataattg tttacactct tcaaatcttt
     5221 cagaaaaaca aatcaataga taaaataatt attgctgcag aacctgatta cttcaatctt
     5281 cttataaagc ttattaaaga atataagcta agtaaagtaa aactgattgt agagggcgga
     5341 aagacacggc aggattctgt ttacaatgcg gcgctttcat caggtgctga tgataatgat
     5401 ttattagtag ttcatgatgc tgcacgggca ttattacctg agaatacatt aactaatgct
     5461 atagaaactg caaaacaaaa aggcagtgct ttggtttgta taaaagcaaa agatacatta
     5521 atcaaaggca aaaaatttgt tgatggttac ctggaacgag atgaagttta ttacgttcaa
     5581 actcctcaga tatttaaata taaagatctt tacagagctt taattaaagc cgaaaaagaa
     5641 aattttatag gaacggatga atcaatgctt gttaagagaa ttggtaaaaa agtaaatatt
     5701 gttgaaggtt ctgtttttaa ttttaagata actaccagtg aagatgttga gttgtttaaa
     5761 aaattagtaa agtaattcaa atcaaatccg cataatttcc tccaataatt ctcaacaatt
     5821 tgtccgggga aagtttcatt tgggtgcctc gaactccagc acttacaaaa atactttcaa
     5881 atatttgcgc agtctcatcc aaataggttg gaaagagctt tttcattcca accggagaac
     5941 atccgccgcg aatatatcct gttaatggga gcagatcttt caattttatc atctcaacct
     6001 ttttattacc gcttgcagcc gcagcttttt ttaaattaag ttcctgtgta acgggaatgc
     6061 aaaaaactat atgccctgtt ttgtctccaa tacaaacaag tgttttaaat actgtttcag
     6121 ggtcagcact gatttttaat gcaaccgtat ctccgcttaa atcgctttca tcaacttcgt
     6181 acgaaacagt ttcgtatttg attttgtgag attcaagaat ccttattgcg ttagttttgt
     6241 tcataagtat tgtggaaagt atgaatatct gcttcggtaa gatttaatga ctgcgcactt
     6301 aataaaaact ttgtgtaatc gttggtagga ttatctatta cagttttagt ttgtccgctt
     6361 tcaacaatgc ttccgttata cataattact atttcatcag aaagatgttt tagaatatta
     6421 agatcgtgtg aaatacatat aattgtaagg ttaaattctt cttttaattt ttttataaga
     6481 ttgagtatgc ccaattgagc ttcaacatcc tgc
//
LOCUS       DASWBY010000068         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_416384, whole genome shotgun sequence.
ACCESSION   DASWBY010000068 DASWBY010000000
VERSION     DASWBY010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416384"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..899
                     /locus_tag="VF870_01465"
     CDS             156..899
                     /locus_tag="VF870_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9250874.1"
                     /translation="MNNETVIKTDKLTKKFGDFTAANELTFEVYKGEIFGFLGANGAG
                     KTTAMKMLIGISKPSSGKANIAGFDVYKDTESIKRSIGYMSQKFSLYEDLTIKENITL
                     FGGIYGLSDKDIETKCNDLLEKLNLQNDANKLVGSLPLGWKQKLAFSTAIFHTPKIVF
                     LDEPTGGVDPITRRQFWDMIYEAAHHGITVFVTTHYMDEAEYCDRVSIMVDGVIKALD
                     SPAGLKRQFNAKSMDEVFVKLARGAKRGE"
     gene            1118..>1641
                     /locus_tag="VF870_01470"
     CDS             1118..>1641
                     /locus_tag="VF870_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9250875.1"
                     /translation="MKQLITFVKKEFHHILRDKRSMLVLLGLPIVQLLIFGFAITTET
                     RNCNIAILDNSKDEATQNIITRIENSTYFDIYRTLSSNDQIEKEFKTGRIKLAIVFPP
                     NFQNDLLHTNKAQIQIITDASDPNQATTLSNYVSAIVLEYQQELNQNKDLPYTINTEI
                     RMLYNPQLKGEFSSV"
BASE COUNT          603 a          260 c          290 g          488 t
ORIGIN      
        1 aattttgatc cggaggaact caaaacatat ttgaaaaatc aaactaatct ggaaatcaaa
       61 aagatagaac cgggaatcga ggattgcttt atgagtttaa tctcaaataa aaataaaact
      121 ctacaagatt taccggcata aactggctta agaaaatgaa taatgaaact gtaattaaaa
      181 cagataaact cacaaaaaaa tttggagatt tcaccgcagc caatgaattg acttttgaag
      241 tttataaagg cgagatcttt ggttttctgg gtgctaacgg tgctggcaaa accacagcaa
      301 tgaaaatgct tatcggtatt tctaaaccaa gctccggaaa agcaaacata gccggattcg
      361 atgtttataa agacacagaa agcattaaaa gaagtatcgg ctatatgagt caaaaatttt
      421 ctctctatga agatttgacc atcaaagaaa atataacctt gtttggcggt atatatggat
      481 taagtgataa agatatcgag acaaagtgta atgatctgtt agagaaatta aatttacaaa
      541 atgatgcaaa taaattagtt ggttccttac cgcttggctg gaaacaaaaa cttgccttta
      601 gcacggcaat atttcacaca cctaaaatag tttttcttga tgaaccaacc gggggagttg
      661 atccgattac cagaagacag ttttgggata tgatttatga agcggctcac catggaatta
      721 ctgtttttgt aacgacacat tatatggatg aagctgagta ctgcgatcgt gtttcaatta
      781 tggttgatgg agtaattaaa gctttggatt caccagctgg actgaagaga caatttaatg
      841 caaaatctat ggatgaggtt tttgtaaagc ttgctcgcgg agccaaaaga ggagaatgat
      901 tttttaatca tcattccgaa cctgtcttta ccggtaggca ggctcgtttc ggaatctctc
      961 tttcgtatta tttagagatg ctgaaacaag ttcagcatta ctaatgctta aaattagttt
     1021 taaacttaaa tatgataaaa taaagagtac tatttttata tagttggaga ttgcttctcc
     1081 cgacttcgtc gggctcgcaa tgacataaaa gaacaatatg aaacaattaa taacatttgt
     1141 aaaaaaagaa ttccatcaca tcctccgcga taaaagatct atgcttgtgc ttttaggttt
     1201 acctattgta cagctgttaa tatttggatt tgcaattaca actgaaaccc gtaattgtaa
     1261 cattgcaatt ttagataact caaaggatga agcaacacaa aatattatca caagaattga
     1321 aaacagcact tactttgata tttacagaac actttcatca aatgatcaaa ttgaaaagga
     1381 atttaagaca gggcgaataa aattagcaat tgtttttccg ccaaattttc aaaacgattt
     1441 attgcatacc aataaggcac aaattcaaat aataacagat gcttctgatc caaatcaggc
     1501 aactacttta tctaattatg taagtgcaat tgtattggag tatcaacagg aattaaatca
     1561 aaataaagat ttgccataca caatcaacac ggaaataaga atgctttata atcctcaatt
     1621 aaaaggcgag ttttcttctg t
//
LOCUS       DASWBY010000069         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_190693, whole genome shotgun sequence.
ACCESSION   DASWBY010000069 DASWBY010000000
VERSION     DASWBY010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190693"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..384
                     /locus_tag="VF870_01475"
     CDS             184..384
                     /locus_tag="VF870_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9250876.1"
                     /translation="MRNTIKVERAKKNITQADLAEAIDVSRLTIHSIETGKFNPSILT
                     ALKMARFFDIPVDELFMLEETD"
     gene            420..1325
                     /locus_tag="VF870_01480"
     CDS             420..1325
                     /locus_tag="VF870_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX9250877.1"
                     /translation="MEKKKGILPAFIAISAGIITVFIAVSDNEPSDCFTWKVKINESV
                     FYLTGSIHGAGEANYPLPETYLKSYKKADKVILELKDDFETLENKIFLYAEKDKLPDG
                     VNLGDSLKSESIYKLREIIGSDKLNKCLGYEAWLLNMFIAGNKSKLCGYDPELAIDKY
                     FHDLATRDKKEIIGLDEIQTQLALFDFDLPIKIQIQILEKAISEMAMKAKGEEELFKA
                     YFENNLVKFEKEFLKPYDFKNPQMKKMYDMVFTKRNTSWVEQFIELSRGNPGSYFVLV
                     GSGHYFGPDNILELLESKGYTIEKI"
     gene            1580..>1765
                     /locus_tag="VF870_01485"
     CDS             1580..>1765
                     /locus_tag="VF870_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250878.1"
                     /translation="MKKLILSISLLLILLQIVAQEIKSFKTSDGETLYFRTVGKGPKI
                     IFLSGGPGFGADGLNVWA"
BASE COUNT          632 a          268 c          317 g          548 t
ORIGIN      
        1 tagagattgt actgattttt tatcagctcc ttttcagata ctgtcttgcc acgaatcctg
       61 actggatatt taaagaaaca accagaagta aatttgctgt ttactatttg tttttttcct
      121 tactattcct ggttggctgg attatttatg tagtaattca atataaaata taagattccc
      181 cttatgcgaa atacaataaa agttgaaagg gcaaaaaaaa acattaccca ggctgattta
      241 gctgaagcta ttgatgtttc aagattaaca atccactcta ttgaaaccgg taaattcaat
      301 ccatcaatat tgactgcact taaaatggca cgcttttttg atatcccggt tgatgaatta
      361 tttatgcttg aggaaaccga ctaaattaaa ataatgataa caaacgtaaa cttagtatta
      421 tggaaaaaaa gaaaggtata ctaccggcat tcattgcaat atcagcaggt ataatcactg
      481 tatttattgc cgtatctgat aatgaaccct ccgattgttt tacatggaaa gtaaaaataa
      541 atgaatctgt tttttattta acaggatcta ttcacggggc tggtgaagcg aattaccctc
      601 ttccggaaac atatcttaaa agttataaaa aagcagataa ggttattctt gaattaaaag
      661 atgattttga aacactggaa aacaaaatct ttttgtatgc agaaaaagat aaattacctg
      721 acggagtgaa cctgggagat tccctgaaat ctgagagtat ttataaatta agagaaatta
      781 tcgggagtga taaattgaat aagtgtttag gatatgaagc atggttacta aacatgttta
      841 ttgcaggaaa taaatccaaa ttatgcgggt atgatccaga acttgccatt gataaatatt
      901 ttcatgactt ggcaaccaga gacaagaaag agatcatagg tttagatgaa attcaaaccc
      961 agttagctct gtttgatttt gacttgccaa ttaagattca aatacaaatt cttgaaaaag
     1021 cgatttctga aatggcgatg aaagcaaaag gtgaagaaga attatttaaa gcatactttg
     1081 agaataatct ggtaaaattt gagaaggagt tcttaaagcc ttatgatttt aaaaatccgc
     1141 aaatgaaaaa aatgtatgat atggtattca caaagagaaa taccagttgg gttgaacaat
     1201 tcatagaatt atccagggga aatccgggtt catattttgt tttagtagga tcaggtcatt
     1261 attttggacc agataatatt ctggaattac tggaaagcaa aggatacact attgaaaaga
     1321 tctaagccag gatatccttg tatggggaga aatattacca actcattttt cgatcaaaac
     1381 accccatatt cgtccaaaac ctggcaacgg accgaccgtt aagaaatcac tcggtaaaat
     1441 ccttccttct ttgaccacat ttcaaacagg tctgataata tttaacatat attggaaaac
     1501 aataaggata taactctagc ttgcatcagt ataatttact cacagatatt ttagtcagtt
     1561 atggatcaga aataattaaa tgaaaaaact catcctgtca attagtctcc tgttgatttt
     1621 actgcaaatt gtcgctcagg agattaaatc attcaaaact tcagatggtg aaacattata
     1681 cttcagaacg gttggtaaag gtcctaagat catattttta tccggaggac caggctttgg
     1741 tgcagatgga ttgaatgtct gggca
//
LOCUS       DASWBY010000070         7317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_265955, whole genome shotgun sequence.
ACCESSION   DASWBY010000070 DASWBY010000000
VERSION     DASWBY010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265955"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VF870_01490"
     CDS             complement(<1..315)
                     /locus_tag="VF870_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250879.1"
                     /translation="MILSEGFFRLSAMAGFLIILLVLLFGYLFFEPFLTEETITIKVT
                     NKAQFGNEPGKYFVFTPDEVFYNSDNYYHSKENADELDKQIYSGATYKVKVVGIYMPW
                     LPR"
     gene            421..1179
                     /locus_tag="VF870_01495"
     CDS             421..1179
                     /locus_tag="VF870_01495"
                     /inference="COORDINATES: protein motif:HMM:NF018659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HEX9250880.1"
                     /translation="MKNIIKNCATILVVIFIIFSQCKCNTNREESPIPANLSILKKTP
                     IKVPEPSGLALSYDESCFWSVGDSDSKVYKLDLNGNIIQSFSIKGEDLEGITIIDSIH
                     LAVILERTREVVVVDTLGKEIKRNKFNLKGNLNEGLEGICFNPKTKDFYFLNEKNPGL
                     LIKTDSSFNEIFRKEIKIAKDYSDIFFSKEDSTLWILSDESKKIIQTDLNGMKIKEFR
                     IDVEQPEGLVVDYKNKKVFVVSDKKEELYEFNLP"
     gene            complement(1186..2583)
                     /locus_tag="VF870_01500"
     CDS             complement(1186..2583)
                     /locus_tag="VF870_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX9250881.1"
                     /translation="MIVNKKSIYTSRVISDLLLLNLSFILAAILAQPFEILLARNYMF
                     ILLLLLNIIWFVNTNLSGFYLDFFTRSFSFQFFNILKLVFFQVGLTVLFIFLTKEDLF
                     TRNFIVFYGLILTLSISLRAAIFKKILISLRRKGKNIRNLLIIGAGEVGKNFKETITH
                     NPDFGYQFSGFLDNVIKDDEVIGNLDQLDKKLKQKEIDEVVIALSSQPTDLLDEIIRI
                     CNINAVRIHIIPDYFRFLSSRFQISSIGNFPIITARQEPLEEANRRFFKRSFDIVFSF
                     LVIALLLSWLYPIIAFLIKMNSKGKVLFIQKRVGAKNEMFECYKFRTLTSESSKETEV
                     FKPVLEGDTRVTGIGNFLRKSNIDELPQFFNVLKGDMSVVGPRPHAIPYQDMYGKIFE
                     EIKMRHNVRPGLTGWAQVNGLRGDVEDEDENRRRTLLRMKYDLWYIENWTMRLDIQII
                     LMTIWQMIKGDTKGV"
     gene            complement(2570..3724)
                     /locus_tag="VF870_01505"
     CDS             complement(2570..3724)
                     /locus_tag="VF870_01505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9250882.1"
                     /translation="MNSNKTAIVHEWLVNYAGSEKVVESFTNIWPDADVYTLVDFLDD
                     DLREIILKGKHAKTSFIQHLPYAKKQHRKYLPLFPKAIEFFNLKNYKLIISSSHSVAK
                     GVRKRKNQLHISYCHSPMRYAWDEADYYLQGANLSSGIKGKIAKVALNYLRKWDVKSA
                     KSVDYFIANSNHIAAKIKRIYNRHADVIYPPVDTQKFSLVTEKEDYYLTASRLVPYKR
                     IDLIIDTFANMLDKKLVVIGSGPEKEEIKAKATPNVDVIGYQDFESLKGYMEKAKAFV
                     FAAEEDFGIIVVEAMACGTPVIAGNYGGTAESVIDGVTGILFPEQTVESIIQAVKRFD
                     VISHSINYKEIRLHSEKFSRSTFEKNIKEYVNDKLKSFQHAKSKKQNDRK"
     gene            complement(3781..4905)
                     /locus_tag="VF870_01510"
     CDS             complement(3781..4905)
                     /locus_tag="VF870_01510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9250883.1"
                     /translation="MTASQKHIKILHLTLRSDWGGAPKHIDILYKNINPDFLIYFAAP
                     ISEPYGTSWYKSVGKERFFELEHRKFSILKLVQLKLFIKNNGIKIIHAHGKGAGLYGR
                     LLKILLPKSIKVIFTFHGLHIDKYSPLKKKLYILYEQFFSRFTDLFISVSNGEKDNCI
                     RHKIFNSQKSKVVYNSIPELINQKSKTDLRVELDLPIEKFIVLSIVRFSFAKNIEDTI
                     AIADLLKPDQRFLIVLVGDGETRAEIESEIKNRKLNNILLPGFKNNPLDYIATSDVYL
                     STSRWEGLPYSLIEASMMGLPSVASDVVGNNEVVKNDFNGKLFKPGNLKAAAQSIIEI
                     CTDKRLSEFYSMNSKKFYNDNFSVDKMITQMQNIYNEIIN"
     gene            4972..5778
                     /locus_tag="VF870_01515"
     CDS             4972..5778
                     /locus_tag="VF870_01515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9250884.1"
                     /translation="MKISVITPTFNSEKTILKNVESIINQSYKYFEHIIIDNRSSDKT
                     IAIIKNLYKDLPSNLRIISEKDSGISEAFNKGIMCAKGDIITILNSDDYYYSGSIFAI
                     VIDALKESQNLIFHGDVLFMDPKYGSYVRRPYGLNKLNTMPLNHPTMFIKKEVYDSIG
                     LYDLNYRYSMDFEFYCRLYKNYPNLGENLCYFDKNPVVIMSSGGESWANEIKSILEVK
                     SAVKKHNLINFNLWLQLQLRLFRTYLKIIFNKIGLTFIVKLWRKIIHSPN"
     gene            5792..7165
                     /locus_tag="VF870_01520"
     CDS             5792..7165
                     /locus_tag="VF870_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9250885.1"
                     /translation="MKIPNIKYSPSELFSFKNSSRTEKAKKNIVLLFILQVFNFFSLM
                     AIVPVTLNYLGKTEYGIWLTLASILSWLINLDFGLGNGLRNKLAEALALNDIKLARIY
                     VSTAYAVFSVGILAAFIIYFTVHGFLNWADLLQAPTEYTALLNTLVLYVIILFLVQFL
                     LKLITSVINADQRPALNGGLSLITNSLTLLVVYLLSLYVKSSLTIFALVSSGIPVVVF
                     LIASLFLFSGHFRNISPSFNYINLKYSSSLVKLGMQFFVIQISSLIVFTTGNIIITQL
                     YGPQSVVTYNVAFKYFYMVPLVFNVALAPFWSAFTEAYVKQEFDWIKNSIRKLVYIWG
                     ILSVIAIVMVIASDFVYKIWVGPQVQVPFTLSVASGVFVIIANWNNIFGYFINGVGKV
                     RLQLFYSIIIAFLNIPLSIFLAKNIGLGVTGVMVSTNICLIFASVWSPIQYHKLISGT
                     AKGIWNK"
BASE COUNT         2449 a         1146 c         1106 g         2616 t
ORIGIN      
        1 tcgcggcagc cagggcatat aaattcccac tacttttact ttgtaagttg cacctgaata
       61 aatttgttta tccagttcat ctgcattttc tttgctatga tagtaattat cagagttata
      121 gaatacttca tccggtgtaa agacaaaata tttacccggt tcatttccga actgagcttt
      181 attagtaact ttaatcgtaa tcgtttcttc ggtaagaaaa ggttcaaaaa aaagatatcc
      241 gaataaaagc acaagaagta taattaaaaa accggccatt gctgataatc ggaaaaatcc
      301 ttcgctaagt atcatttatt tatttctcct tcaaaaaact taatcgtaaa cattatattt
      361 agaaacctta taaataaatt ttataaataa ttacataaaa tccatacata atcgatttat
      421 atgaaaaaca ttatcaaaaa ttgtgcaaca atattagttg ttatttttat aatattctca
      481 caatgcaaat gtaatactaa cagggaagaa tcgccaatac cggcaaattt atccatccta
      541 aaaaaaacgc ctataaaagt tcctgaacca tcgggtttag cattatccta tgatgaaagt
      601 tgtttttggt cggttggcga tagtgatagt aaggtttata agctggattt aaatggtaat
      661 attatccaat ctttttctat aaaaggtgaa gatcttgagg gaataacgat aattgactca
      721 atacatcttg ctgttattct tgaacgaacg agagaagttg tagtagttga tactttagga
      781 aaagaaatta agcgaaataa atttaattta aagggaaatc tcaatgaggg cttagagggt
      841 atttgtttta atcctaagac aaaagatttc tatttcttaa atgaaaaaaa tcccggtttg
      901 ctgattaaaa cagacagcag ttttaatgag atatttagaa aagaaattaa aattgccaaa
      961 gattattcgg atatcttttt ttctaaagaa gacagtacgc tctggatttt aagtgatgaa
     1021 tcaaaaaaga ttatccagac ggatttaaat ggaatgaaaa ttaaagaatt cagaatagat
     1081 gttgaacaac cagaaggatt agtagttgat tataagaata aaaaagtatt tgtggtttcg
     1141 gataaaaagg aagagctgta cgaatttaac ttaccttaaa aatgattaga cacctttagt
     1201 atcaccttta atcatttgcc agatagtcat tagaataatc tgaatatcaa gtcgcatagt
     1261 ccagttttct atataccaaa ggtcgtactt cattcttaga agagttctgc gtctattttc
     1321 atcctcatct tcaacgtcac cgcgtaaccc atttacttgt gcccagccag ttaaacccgg
     1381 gcgaacatta tgccgcattt taatttcttc aaatattttt ccatacatat cctggtaggg
     1441 gattgcgtgc ggtcttgggc caactacaga catatcacct tttagtacat taaaaaactg
     1501 aggaagttca tctatgttag attttcttaa gaagtttcct ataccggtaa ctcttgtatc
     1561 tccttctaaa accggtttaa aaacttccgt ctcttttgat gattcggatg taagagttct
     1621 aaatttgtag cattcaaaca tttcgttttt agctcctaca cgtttttgaa tgaacaatac
     1681 tttgcctttt gaattcatct taattaaaaa tgcgattatc ggataaagcc agctaagtag
     1741 caaagctata actaaaaatg aaaacacaat atcaaatgaa cgcttaaaaa atcttctgtt
     1801 agcttcttct aatggttctt gccgtgcggt gattatagga aaattgccta tagagcttat
     1861 ttgaaatctg cttgataaaa accgaaaata atccggaatg atatgaattc ttactgcatt
     1921 tatgttgcaa attcttatta tctcatctaa taaatccgtt ggttgagatg aaagtgcaat
     1981 tacaacctca tcaatttctt tttgctttaa ctttttatcc agttgatcaa gattgccgat
     2041 tacttcatca tctttaatta cattatcgag aaatccagaa aactgatatc cgaaatcagg
     2101 gttgtgagtt atagtttctt taaaattctt tccaacttct cctgctccga taattaaaag
     2161 atttcttatg tttttacctt ttcttcggag tgatattaga atttttttga agatagctgc
     2221 acgcagactg attgatagtg tgagaattaa tccatagaac actataaaat ttcttgtaaa
     2281 aagatcttct tttgttagga agataaataa tactgtcaat ccaacttgaa agaaaacaag
     2341 ttttagaata ttaaaaaatt gaaatgaaaa tgatctggtg aaaaaatcta aataaaatcc
     2401 cgaaagatta gtgtttacaa accaaattat gttcagtaaa agcaaaagta taaacatata
     2461 gttgcgcgcc agtaatattt caaaaggctg agcaagtatt gctgccagga taaatgacaa
     2521 attgagcagc agcaaatcag aaattactcg tgatgtataa attgattttt tatttacgat
     2581 cattttgttt ttttgatttt gcgtgttgaa aactttttaa tttatcgttt acatattctt
     2641 tgatgttttt ttcgaaagta gatctgctaa atttttctga gtgcaatctg atttctttat
     2701 aattaattga atgagatatt acatcaaatc ttttaactgc ttgaattata gattctaccg
     2761 tttgttcagg aaaaagaatt ccggttacac cgtcaataac agattccgcg gtaccgccat
     2821 aattacctgc aattactggt gttccgcatg ccattgcttc aacaacaata atcccaaaat
     2881 cttcttctgc tgcaaagaca aaagcttttg ctttttccat atagcctttt agactctcaa
     2941 aatcctgata tccgattaca tcaacatttg gtgttgcttt agctttaatt tcttcttttt
     3001 caggaccgct tccgataaca acaagttttt tatcaagcat atttgcaaag gtatcaatta
     3061 tcaaatcaat tcttttgtaa ggtacaagtc tggaggcggt taaataataa tcttcttttt
     3121 ctgtgactaa ggaaaatttc tgagtatcaa caggtggata aattacatct gcatgccggt
     3181 tgtaaattcg ttttatttta gctgcaatat gatttgaatt tgcaataaag taatcaacac
     3241 tctttgcaga ttttacatcc cattttctta aatagtttaa tgcaactttg gcaatcttac
     3301 ccttgattcc tgaagacaaa tttgcacctt gtaaataata gtctgcttca tcccaggcat
     3361 atctcatagg cgaatgacaa taactaatat gcaattgatt ttttcttttt cttactcctt
     3421 ttgcaacaga atgtgagctc gaaataatca acttataatt ttttaagtta aaaaattcaa
     3481 ttgctttagg aaacaaagga agatacttac gatgttgttt tttggcataa ggcaaatgct
     3541 gaataaaaga agtctttgca tgtttccctt taagtataat ttctcgtaaa tcatcgtcta
     3601 agaaatcgac taaagtatag acatcagcat ccggccaaat attcgtaaaa gattctacaa
     3661 ctttttcaga acctgcataa ttaacaagcc actcgtgtac aattgctgtt ttatttgaat
     3721 tcattatttt attcgattat taaatcaact gtatcgttta atttaaaaat tataaattat
     3781 ttagttaata atttcattat agatattttg catttgtgta atcattttat ccactgaaaa
     3841 attatcatta taaaatttct ttgaattcat actgtagaat tcagataatc ttttatcagt
     3901 acaaatttca ataatactct gtgctgctgc ttttagatta cctggcttaa ataactttcc
     3961 attaaaatca tttttaacaa cttcattatt gccaaccaca tctgaagcaa ctgaaggcag
     4021 acccatcatc gatgcttcaa ttaaagaata aggcaatcct tcccatctgg aagtagataa
     4081 ataaacatcg ctggtagcaa tataatccag cgggttattt ttaaacccgg gaagtaaaat
     4141 attatttagc tttctattct taatttcact ttcaatttcc gctctcgttt cgccgtcgcc
     4201 aaccaaaaca ataagaaatc tttgatcggg tttaagcaga tcggctatag caattgtgtc
     4261 ctcaatgttt ttagcaaatg aaaatcgaac tatggataat acaataaatt tctcaatagg
     4321 taaatcaagc tcaactctta aatctgtttt tgatttctga tttatcaatt cgggaattga
     4381 gttataaaca acttttgact tttggctatt aaatatttta tgccttatac aattatcctt
     4441 ttcgccattt gatacactaa taaaaagatc agtaaatctt gaaaaaaact gttcatataa
     4501 aatatataac tttttcttaa gtggactata tttatctatg tgtaaaccat gaaaggtgaa
     4561 tatcacttta atacttttag gtaaaagtat ttttagtaat ctgccatata atcccgctcc
     4621 tttaccatga gcatgaataa tttttattcc gttattcttt ataaataatt ttaattgaac
     4681 aagttttaat attgaaaact tacgatgttc aagctcaaaa aaccgttcct tgccaacaga
     4741 tttataccaa ctagtcccgt aaggttcact tattggtgcg gcaaaatata ttagaaaatc
     4801 aggattaata tttttgtaca aaatatctat atgttttggt gcaccgcccc aatccgacct
     4861 gagagttaaa tgcagtattt taatatgttt ttgagaagca gtcatcagtt atagaatcaa
     4921 acgatatttg tatatttatg cgcaaataaa tttctggttt caatattatc tatgaaaatt
     4981 tctgtaatta caccaacatt caattctgaa aaaaccattt taaagaatgt tgaatcaatc
     5041 ataaaccaat cctacaaata ttttgaacat attattatag ataatcgaag cagcgataaa
     5101 actattgcga ttataaaaaa tctttataaa gatttaccct caaatttaag gattattagt
     5161 gagaaagata gcggaatatc agaagccttt aataaaggaa taatgtgtgc aaaaggtgat
     5221 attataacaa ttcttaattc agatgattat tattattccg gtagcatttt tgcgattgta
     5281 attgatgcgc tcaaagaatc tcagaatcta atttttcatg gagatgttct gtttatggat
     5341 cccaaatacg gatcctatgt aagaagaccc tatggactta ataaactaaa cacaatgccg
     5401 cttaatcatc caactatgtt tattaaaaaa gaagtgtatg attcaattgg attgtatgat
     5461 ttaaattatc gttactcgat ggattttgag ttttattgcc ggttatataa aaattatcct
     5521 aatcttggtg aaaacctttg ttactttgat aaaaatcctg tggtaataat gagttccggc
     5581 ggagaatcct gggcaaatga aatcaaatct attctggaag taaaaagtgc agttaaaaaa
     5641 cataatctta ttaattttaa tttatggctg cagcttcaat taagattatt cagaacttat
     5701 ttaaaaatta ttttcaacaa aatcggcctg acttttatag ttaaattatg gagaaaaatt
     5761 attcacagcc ctaattaatc taatatttaa tttgaaaata ccgaacataa aatattcacc
     5821 atcagaacta tttagtttta agaattcttc cagaactgaa aaagcaaaaa agaacatcgt
     5881 actgcttttt attttacaag tatttaactt tttttcattg atggcaatag tgcctgttac
     5941 tcttaattat cttggtaaaa cagaatacgg gatttggctt acacttgctt caattttaag
     6001 ttggttgata aatcttgatt ttggtcttgg caacggatta agaaacaaac tggccgaagc
     6061 ccttgcgctt aacgatataa aattagcccg aatttatgta agcacagctt atgcagtttt
     6121 ttcagttgga atattagctg catttataat ttactttaca gtccatggtt ttcttaattg
     6181 ggctgatctt ttgcaggcgc caacagaata tacagctttg ctgaatactt tagttttata
     6241 tgtaattatt ttatttcttg ttcagttttt attgaaactt ataacctcag taattaatgc
     6301 agatcaaaga ccggcgctta atggaggatt aagtttaata actaactcgc ttactttgtt
     6361 ggttgtttat cttctttcgc tttatgttaa atcatcatta acaatatttg cattggtttc
     6421 aagcggaatt cctgtcgtag tttttttaat tgcttcgcta tttcttttta gcggtcactt
     6481 cagaaatatt tctccatcgt tcaattacat taatcttaag tactcatcaa gtctggtaaa
     6541 acttggaatg caattttttg ttattcaaat atctagctta atcgttttta caactggcaa
     6601 tattattatc actcaactct atggaccgca atcagtagtt acttataatg ttgcatttaa
     6661 atatttttat atggttccat tagtttttaa tgttgctctt gcacctttct ggtcggcttt
     6721 tacggaggct tatgttaagc aagaatttga ttggataaaa aattctatac gaaaattagt
     6781 atacatctgg ggtattttat cagtaatagc tatcgttatg gttattgctt cagattttgt
     6841 ttataaaatt tgggttggac ctcaagtgca agttccattt acgctttctg ttgcctccgg
     6901 agtatttgta atcatagcaa actggaataa tatatttggt tattttatca atggagttgg
     6961 taaagttcga ctgcaacttt tctattcaat cataatagca tttttaaata ttcctctttc
     7021 aatatttctt gcaaaaaaca taggacttgg tgttacgggt gttatggttt caacaaacat
     7081 ctgtttgata tttgcttctg tttggtcgcc aatacaatat cataaattaa tttcaggaac
     7141 tgcaaaagga atatggaaca aatgatttgt gaaatttgta attcaaaggc acagcaggta
     7201 tttacttcta aagtgttaag aaagcacgac gttaaatatt ttaaatgtga taattgcggg
     7261 catttattta cggaggaacc attctggctg gatgaggcct attcaagaag tataaat
//
LOCUS       DASWBY010000071         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_78100, whole genome shotgun sequence.
ACCESSION   DASWBY010000071 DASWBY010000000
VERSION     DASWBY010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78100"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VF870_01525"
     CDS             <1..572
                     /locus_tag="VF870_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX9250886.1"
                     /translation="KNINHILVAGKNGLDIGNQCGGWTISWQGSSGSITPGTTVYQGI
                     KNTVPGSTTVSYSFNGSNTQGADVAVIVVGETPYAEGNGDRNDLSLSSEDITTINNIK
                     NAGVPYVIVLISGRPMIITDVLNDCNAFIAAWLPGTEGQGIADVLFGDYNFKGKLSHS
                     WPRTMNQIPINSGDANYDPLFPYGFGLTY"
     gene            605..2842
                     /locus_tag="VF870_01530"
     CDS             605..2842
                     /locus_tag="VF870_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9250887.1"
                     /translation="MKKYFITLFSILVIASSGFINLPNHQSDEQKISARVDSVLSLMT
                     LDEKIGQLNQLSYGIGWGPTVKVSVPDEYKQMIKDGKIGSFLNAIGAEFTYELQKIAV
                     QESRLKIPLLFGLDVIHGFKTTFPVPLGEAASWQPEIVELSAHYQALESSSAGIHWTF
                     SPMVDIARDPRWGRIMEGSGEDTYLGSLMSAARVRGYQGNLSNENIIACVKHYAGYGG
                     AEGGRDYNTVDFSDRTLRDIYLPPFKAAVDAGVETVMASFNEIGGIPSSGSKYLLTDI
                     LRDEWKFKGFVVSDWNSIGEMINHGFAADLKNAGEISINAGLDMDMESRSYITYLKEL
                     VEEGKVKESVVDESVKRILRVKFLLGLFDDPFRYCSKEREQNNIMTTEIKNASLDVAK
                     RSFVLLKNKNSILPLKKDYNKIAVIGPLADSKIDPLGEWNALGIKEDVISVLEGLKNY
                     VGDKSEILYAEGCKITDPSKDGFKDAVEKANNSDVIILCVGESRSMTGEAYSRSSLDL
                     PGVQEDLVKELLKTGKPIIAVLMNGRPLSINWLNDNVDGILETWFAGTMTGDAVSQVL
                     FGDYNPSGKLPVTFPRSVGQVPIYYNHKNTGRPPNAKERYTSKYFDISITPLYPFGYG
                     LSYTTFEYSDISLNKRELTNKDSILVSVKVRNTGDYDGEEVIQLYIQDLFGSVTRPVK
                     ELKGFKKVMLNRGEEKTIDFYIYEKDLRFTGADMNFKSEPGMFKVYVGTNSADLKESQ
                     FELIQ"
BASE COUNT         1045 a          496 c          629 g          949 t
ORIGIN      
        1 caaaaaatat aaatcatatt cttgtagcag gtaaaaatgg acttgatata ggaaatcaat
       61 gtggcggatg gacaatttcg tggcaaggga gcagtggaag tataactcct ggaacaacag
      121 tctatcaagg gatcaaaaat actgttccag gttctacaac tgtaagttat tcatttaacg
      181 gcagcaacac tcaaggtgct gatgttgctg ttatagtagt cggagagaca ccttatgccg
      241 aaggtaatgg cgatcgaaac gatctttcct tatcatctga ggatattact actataaata
      301 atattaagaa tgcaggagta ccttacgtaa ttgttttaat atctggcaga ccaatgataa
      361 ttaccgatgt gcttaatgat tgcaatgcat ttattgctgc gtggctgccg ggtacagaag
      421 gacaaggaat tgccgatgtg ctttttggcg actataattt taaagggaaa ttatcacaca
      481 gctggccaag aactatgaat caaattccga taaattcagg tgatgctaat tacgatccac
      541 tatttcctta tggatttgga ttaacttatt aattaataaa aaaatattta ttgcagagta
      601 tataatgaag aaatacttta tcactttatt tagcattctt gttatcgcat ccagcggatt
      661 tattaattta cccaatcacc aatcagacga acaaaaaatt tcagcaaggg tcgactcagt
      721 tttatcttta atgacacttg atgaaaaaat cggacagctt aaccagctct cttacggcat
      781 aggctggggt ccaacagtaa aagtttctgt acccgatgaa tataagcaaa tgattaaaga
      841 cgggaagatc ggttctttcc ttaatgctat cggcgcggag tttacttatg agttgcagaa
      901 aattgctgtt caagaatccc gactaaaaat tcctttatta tttggtttgg atgttattca
      961 cggatttaaa actactttcc ccgttccact tggagaagcc gcatcatggc agccggaaat
     1021 tgttgaactc tccgctcact atcaggcgtt agaatcatca tcagctggaa ttcactggac
     1081 attttctcca atggttgata ttgcaaggga tccccgctgg ggaagaatta tggaaggttc
     1141 cggtgaagat acgtatctcg gttctttgat gtccgccgcc cgtgtacgtg gttatcaagg
     1201 taatttatca aacgaaaata ttatcgcctg cgtaaaacat tatgcaggtt atggcggtgc
     1261 agaaggcgga agagattaca acacggttga tttttcagat agaactctac gtgatattta
     1321 tcttccacca tttaaagccg cagttgatgc cggtgttgaa acagtaatgg cttcttttaa
     1381 tgagataggt ggaattcctt cttcaggcag taagtatctg ctcacagata ttttaagaga
     1441 tgaatggaaa tttaagggat ttgtggtaag cgactggaat tcaattggag aaatgattaa
     1501 tcacggtttt gctgctgatt taaaaaatgc aggtgaaatt tcaattaatg ccggactgga
     1561 tatggacatg gaatcacgtt cttatatcac ttatcttaaa gaacttgttg aagaaggaaa
     1621 agtaaaagaa tctgttgttg atgaatctgt aaaacgaatt ctcagagtta aattcttgct
     1681 tggtttattt gatgatccat ttcgttactg tagtaaagaa cgtgaacaaa ataatataat
     1741 gacaacggag attaagaacg catcgcttga tgttgccaaa cgatcatttg ttttattaaa
     1801 aaataagaat agcatccttc ctcttaaaaa ggattataat aagattgctg ttattggccc
     1861 gcttgccgat tctaaaattg atcctcttgg agaatggaac gcgctaggta tcaaagaaga
     1921 tgtgatatct gttttggaag gattaaaaaa ttacgttggt gataaatccg aaatacttta
     1981 tgcagagggt tgtaaaatta ctgacccatc aaaagatggt tttaaagatg cagttgaaaa
     2041 agcaaataat tccgacgtta taattctgtg tgttggggaa agcagaagta tgacaggtga
     2101 ggcatacagc agatcttcac ttgatttacc gggagttcag gaagatcttg taaaggaatt
     2161 gctgaaaaca ggtaaaccaa ttattgcagt tttaatgaac ggaagacctt tgtctattaa
     2221 ctggctgaat gataatgttg atggaatttt agaaacctgg tttgcaggaa caatgacagg
     2281 agatgctgtt tctcaggttt tatttgggga ctacaacccc tcaggtaaac tgcctgttac
     2341 tttcccaaga tcagttggtc aggttccgat ctattataat cataaaaata ccggaagacc
     2401 cccaaatgca aaagaaagat atacatcaaa atattttgat atttcgataa ccccacttta
     2461 cccttttggg tatggattaa gttatacaac ttttgagtat agtgatattt ctttaaataa
     2521 aagagaatta acaaataaag attcaatact tgtatctgta aaagtcagga ataccggtga
     2581 ttatgacggt gaagaagtta ttcaattata tattcaggat ttatttggaa gtgttacacg
     2641 tcctgttaaa gaattaaaag gatttaaaaa agtaatgcta aatcgcggtg aagaaaaaac
     2701 aattgacttt tatatttacg aaaaggattt aagatttact ggcgctgata tgaattttaa
     2761 atcagaaccc ggaatgttta aggtttatgt tggaacaaat tcagcagatc taaaagaaag
     2821 tcaatttgaa ttaattcaat aagggatttg attaaaattc atcccgagcg aaaaagaagc
     2881 atgacatcat accggacata actaaagtca cacttcgact tcgctcagta tgacgaagaa
     2941 aactgttagg acattatatg aaaagaaaaa tattatctaa aattattata atgctattcg
     3001 cactaagctc aatttcattt acgaactgca gcaaggatga ttcaacaaca actccggata
     3061 ataatgtaat tcctccaatc cccggttatt ctttagtatg gaacgatgag tttgaaaat
//
LOCUS       DASWBY010000072         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_642224, whole genome shotgun sequence.
ACCESSION   DASWBY010000072 DASWBY010000000
VERSION     DASWBY010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642224"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VF870_01535"
     CDS             complement(<1..567)
                     /locus_tag="VF870_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9250888.1"
                     /translation="MDKQPHLKRVLGLSDLSFFLIAALVNLNSVPVVASSGPVALVLW
                     LAGFFLFFIPQAIAVLEFTRRAPQEGGIYVWNKKAWGDFHGFVSGWAYWVNNIFYVPT
                     LLFYIIGFILYIGGSTTASLASNPFFMMSVSLGLLWVITYLNIRGFGVGKWIQNLGAS
                     GTFITAATILVIGIITAFTRGSSNEISIA"
     gene            complement(702..1412)
                     /locus_tag="VF870_01540"
     CDS             complement(702..1412)
                     /locus_tag="VF870_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9250889.1"
                     /translation="MSDEVFDQAKILLVEDEENLALGLEYNLTAEGYDVKWAKDGKEA
                     LKFFDEGKFDLIVLDIMLPYFNGFEIAKHIRENHPQMPILMLTARTQTEDRVKGLELG
                     ADDYLTKPFHLKELLLRIKGMLKRKSWYQKVVIDNPIYKFGDCEINFENFKCRKGKID
                     FQLTSYEAMIMKYLIENKDKVVNRKELLENVWNMNPDIETRTVDNFIARLRKHFEDDP
                     SDPKYIVSVRSAGYMFQV"
     gene            complement(1409..>2365)
                     /locus_tag="VF870_01545"
     CDS             complement(1409..>2365)
                     /locus_tag="VF870_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9250890.1"
                     /translation="VIFILAQLVWLAVLLLWIYWYVSNNIIFEQVGEKVAPQIVYDAP
                     SVFPFVGGIVLLTGLSVSIVLIFRHLNIQIKLNVLYDNFIANVTHELKSHLSSIQLYL
                     ETLNSRDVPEEKKKEFYTLMMRDSERLKNLINSILEIASMDKNKFKSDFEVYKADETI
                     KRIILESAEQFKLSPDTIKFSGAAGCDVRLNRTSIKTLFDNLVDNSIKYSTSALQINI
                     LFKRNSKKIEIDFSDNGIGIPHEQIKKIFQRFYRIYDNDIPNVKGTGLGLYVVREIVK
                     NHKGKITAYSEGKGKGSRFKIELPIYIEKKKAISEKKKDLIK"
BASE COUNT          746 a          463 c          360 g          796 t
ORIGIN      
        1 agcaatagaa atttcgtttg atgagcctcg tgtaaatgcg gtaataattc caataactaa
       61 tattgttgcc gcagtaataa atgttcccga tgctccaaga ttttgtatcc atttgcccac
      121 tccaaagccg cggatgttta aatatgtaat aacccagagc agcccgagtg atacactcat
      181 cataaaaaat ggatttgaag caagagatgc tgttgtgctg cctcctatgt atagtataaa
      241 tccgataatg taaaataaaa gtgttggaac ataaaaaata ttattaaccc agtatgccca
      301 tccggaaaca aatccatgaa aatcacccca cgctttttta ttccacacat aaatgccgcc
      361 ttcttgtggt gccctcctgg taaactctaa aacagcaatt gcctgtggaa tgaagaaaag
      421 aaaaaatcct gcaagccata acaccagggc aacagggcca gagcttgcta caaccggaac
      481 actatttaaa ttaactaaag cggcgattag aaaaaatgaa aggtcggata atccaagtac
      541 ccgtttaaga tgaggttgtt tatccaattc ctgtgtaatt ctccggattt tttttgtaaa
      601 tattattcaa tcagagttta ataacaatag gaaaaaatat ttatttgtta atcagataaa
      661 gcgagcgaga agaatttata aatgcgtaaa taatcttaaa cttaaacctg gaacatataa
      721 ccggcacttc taacacttac aatatattta ggatctgatg gatcgtcttc aaagtgcttg
      781 cgcaatcttg caataaaatt atcaacagtg cgggtttcaa tgtcgggatt catattccag
      841 acattttcta ataattcttt tcgatttaca accttgtctt tattctcaat aagatatttc
      901 attatcattg cttcgtatga tgtaagctga aaatcgattt tgccttttct gcatttaaag
      961 ttttcaaaat ttatttcaca gtcgccaaac ttatatatag gattatcaat cacaactttt
     1021 tggtaccagc tttttctttt cagcattccc ttaatcctca gtaacagttc tttaagatga
     1081 aaaggctttg taagataatc atcggctccc agctcaagtc cttttacacg atcttccgtt
     1141 tgtgttctgg ctgttagcat tagtatcggc atctgcggat gattttctct tatgtgtttg
     1201 gcaatttcaa atccattaaa gtatggcagc ataatatcaa gcacaatcaa atcaaatttt
     1261 ccttcatcaa aaaatttgag cgcctctttc ccatcctttg cccacttaac atcatatcct
     1321 tcagcagtaa ggttgtattc caaacccagc gcaagatttt cttcatcttc caccaaaaga
     1381 atttttgcct gatcaaaaac ttcgtcgctc attttattaa atcctttttc ttttctgata
     1441 tagctttttt cttttctata tatataggca actcaatttt aaacctggag cccttcccct
     1501 tgccttcgct ataagcggtt attttcccct tgtggttttt aactatttca cgaaccacat
     1561 agagtcccaa tcccgttcct ttaacatttg gaatatcgtt atcataaatg cggtaaaatc
     1621 tttggaatat tttttttatt tgttcgtgcg gaatcccaat gccgttatca gaaaaatcga
     1681 tttcaatttt ctttgaattt cgcttaaaca aaatatttat ttgcaaagcc gatgtgctgt
     1741 attttatgga attatcaaca agattatcaa acaaagtttt tatagatgtt ctattcagtc
     1801 taacatcgca gccggcagct ccgctaaatt ttatagtatc aggacttagc ttaaattgtt
     1861 ctgctgattc taagattatt cgttttattg tttcgtctgc tttataaact tcaaaatcac
     1921 tcttgaactt attcttatcc atcgacgcaa tttcaagaat tgagtttatc aaatttttta
     1981 atcgttccga gtcccgcatc attaaagtat aaaactcttt tttcttttct tcgggcacat
     2041 cgcgggagtt tagtgtttct aaataaagct gtattgatga caaatgagat tttaattcgt
     2101 gagtgacatt agcaataaaa ttatcataca gtacattcaa ttttatttga atatttaaat
     2161 gacgaaagat taaaacaatg ctaacagata atccggtgag taaaacaatt ccgcccacaa
     2221 aaggaaaaac acttggtgcg tcatatacaa tttgtggagc aactttttca ccaacctgct
     2281 caaatattat gttgtttgat acgtaccagt aaatccagag cagtaaaaca gccagccaca
     2341 caagctgtgc aagtataaaa atcac
//
LOCUS       DASWBY010000073         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_78136, whole genome shotgun sequence.
ACCESSION   DASWBY010000073 DASWBY010000000
VERSION     DASWBY010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78136"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..>1884)
                     /locus_tag="VF870_01550"
     CDS             complement(106..>1884)
                     /locus_tag="VF870_01550"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX9250891.1"
                     /translation="KQLLKSAAIFIAAVVVGLGIQSDNFTQVWEYSPYSTRGTESILD
                     KESGSKQKTETSFYDYATSWSFSPGEVLTWIVPSYYGFGKSVYNGPLSQNRDVEVNTY
                     FGQMPFVDVAMYMGVIVFFLAIFSMIINWKDPFVRYLTILAVIATLISFGRTFPLFYD
                     LMFHYFPFFDKFRVPSMILVLVQLSLPILAGLGIAKLISLKKENDKKFDTLVRNIFFG
                     LGSIFVLSILLASPIKSWFINRIIESGEKGTQLQQIHDYMAEMFLNDTRLAFFFSAAV
                     FGLIYAYLKSFVSKDLMITAIIVLSLIDIFRINHRGETLKDSSNIEQLFQKPEYVSAI
                     KALNDKSTFRILNLKKDRSLGSVQQNSNYNAYFLLQDLYGYSGIKPRAYQDYMDVLGS
                     PANQTLWRMTNTKYIILDNQVNLAGLVPIYSGNKTFVYENINALPRAYFVNSVEKASA
                     MEILNKVKNNQFDPREVAFVEDAEIKVDRPDSTASVKIEKYQDENIYLNVNASGKNFL
                     FLGDTYVGKGWKAYVDGNATTIYKANHNFRGIIVPKGNHKIHFEYLPESFVITKNVAL
                     VLSSLVVLGLFISVGLQFKKKKEVSE"
BASE COUNT          624 a          347 c          284 g          629 t
ORIGIN      
        1 tttttcaaac agcatccacg aagtatttga aaaatatttc ttaaacgagt ctgatcgaca
       61 attaattttc gtatctattt tattttctac ctccaaagat attttctact cgctaacttc
      121 ttttttcttt ttaaattgta atccgactga aataaataat cccaaaacaa ctaacgaact
      181 caaaactaag gcaacatttt tagttattac aaacgactct ggcaaatact cgaagtggat
      241 tttgtggttt ccttttggaa cgattattcc tctgaaatta tgatttgctt tataaattgt
      301 ggttgcattt ccatcaacat aagccttcca acctttgccg acataggtat caccaagaaa
      361 cagaaagttt tttccgcttg cgtttacatt taaataaata ttttcatcct gatatttttc
      421 aattttgacg gatgcagtac tgtctggtct atcaacttta atttctgcat cttcaacaaa
      481 cgcaacttct ctgggatcaa attgattgtt ttttacttta ttcagtattt ccattgcact
      541 ggctttttcg actgagttaa caaagtaagc tctcggcaat gcattaatat tttcataaac
      601 aaaagtttta ttacccgaat aaatgggaac caatcctgcc aggtttactt gattatcgag
      661 aattatatat tttgtgtttg tcattctcca caaagtttga tttgccggac ttccaagtac
      721 atccatataa tcctgataag ctcttggttt aattccagaa taaccatata aatcctgcag
      781 caagaagtat gcattgtagt ttgaattttg ttgaacagat ccaagagatc tatctttttt
      841 aagatttaaa atcctgaaag tagatttatc atttaaagct ttaattgcag aaacatattc
      901 gggtttttga aataattgtt cgatatttga gctatccttt agtgtttctc cgcgatgatt
      961 tattctaaag atatctatca aagaaagaac tatgattgct gttatcatta aatctttgct
     1021 tacaaaagac tttaaataag cgtagatcaa tccaaaaact gcagcggaaa agaagaatgc
     1081 caatcttgta tcattcaaaa acatctctgc catataatcg tgaatttgct gcaactgagt
     1141 tcccttctca cccgattcaa ttatcctgtt aatgaaccag ctttttatag gcgaagcaag
     1201 taaaatagat aatacaaaaa tcgaaccgag cccgaagaaa atatttctaa caagggtatc
     1261 aaacttttta tcattctctt tttttaatga gattaatttt gcaatcccaa gtccggcaag
     1321 tattggtaaa cttaattgaa caagaaccaa aatcattgat ggaactctga acttatcaaa
     1381 gaatgggaaa tagtgaaaca tcaaatcata aaataaggga aatgttcttc caaaggatat
     1441 aagtgttgct attacggcaa gaatagttaa gtatctaaca aatggatcct tccaatttat
     1501 aatcatcgaa aagattgcga ggaagaaaac tataacaccc atgtacatcg caacatcaac
     1561 aaagggcatc tgcccaaagt aggtatttac ctctacatca cgattttggg aaagcgggcc
     1621 attgtaaact gactttccaa atccatagta agacggaacg atccaggtta aaacttcacc
     1681 aggtgaaaat gaccagcttg ttgcgtaatc ataaaatgat gtttctgttt tttgcttaga
     1741 tccggactcc ttgtccaaaa tactttctgt acctcgtgta gaatatggcg aatactccca
     1801 cacctgagta aagttatctg attgtattcc taatcctaca actacagcag caataaatat
     1861 tgcggctgat tttaataact gctt
//
LOCUS       DASWBY010000074         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_40557, whole genome shotgun sequence.
ACCESSION   DASWBY010000074 DASWBY010000000
VERSION     DASWBY010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40557"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF870_01555"
     CDS             <1..411
                     /locus_tag="VF870_01555"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX9250892.1"
                     /translation="RIATSAKNQNFAGTDFSYDDMESKPFSAKYTPKLIETGANVYTL
                     ELTPQAQSDYSKIIVTINKTNFYPETMDYYDKGNTKIKSAKYVFSKVGNYWNAQEIEM
                     TDFKKNHKTKMQMSDVKYDTGLTDDDFTVRKLIQ"
     gene            440..994
                     /locus_tag="VF870_01560"
     CDS             440..994
                     /locus_tag="VF870_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9250893.1"
                     /translation="MKTISGYLLKDVLGWKIKGEFPDIKKSITIFAPHTAHIDALYGK
                     LGIAQMGVRYLFLSKKELFFFPMNIVMKKFGSMAVRGVKDENAIYKVVKILNENDELH
                     VVISPEGSINRVTNWNKGFYYMAQKANVPIVVTSLDYKKKEMEVKGVIYDLNDYVTVI
                     KQINQMYNGVTGKVHQNFSLSSET"
     gene            998..1870
                     /locus_tag="VF870_01565"
     CDS             998..1870
                     /locus_tag="VF870_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9250894.1"
                     /translation="MNKSVALVLSSGGSKGLAQIGVCYALEKHGFQISSIAGSSIGSV
                     IGGTYSMGKLPEFTAWVKSLDRKTVWGLMDFTISTNGLLKGARVLEKMKTFIPDMNIE
                     EMGIPFSAIAVDILNEKVEVFNSGSFYEAIRASIAIPTLMTPVISGDKILVDGGVLSP
                     IPIEYVTRQPEDILVVVNLYGDKKNDLPDTLSTVNKQHKVIPNNLLKIITKANDTGDK
                     KSMWYYSLLKATTSEMIHKIAKQTIELFKPDILINIPYDSSDTFDFHKAEELIKIGEN
                     AANEAISNYLNHEL"
     gene            1867..2550
                     /locus_tag="VF870_01570"
     CDS             1867..2550
                     /locus_tag="VF870_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9250895.1"
                     /translation="MKSSVVICAYNEDKTIRDVLVSVSDCKIFDEIIVVNDGSADNTK
                     EIIIDCKNELDIIDIHLPENKGKGFAMATGIEHASNELIVFCDADLSNLKREHFLQLI
                     KPIIRSDAKMVLGQPTETLINFHINPFKVLTGQRAVLRSDVLPIVEDMKTSKFGVETL
                     INLYYEAQNKKVKHVKLKGLKHPTKFNKTNLSGAMKAFLFEGIQIALIVFNNLNLITK
                     IISNRINNL"
     gene            2683..3960
                     /locus_tag="VF870_01575"
     CDS             2683..3960
                     /locus_tag="VF870_01575"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEX9250896.1"
                     /translation="MTDERFLLTDKKDWAWNENRLTLKLDKKISNRSKFYSEVWLRNI
                     GLPNITSSADLYNKGIVDPINFELREAYVQIFGFLSKNLDITIGRQRIVWGTADKLNP
                     TDNLNPYDLEDILDFGRHRGSDAVSLNYYINNDFSLQGVYIPIFQPANMPIGIYADAL
                     SPEMDLPQGMVLNEFSDTILMPRYNLAESSTAGLKFKGFVKGVDFSLSYVWGYDGLPF
                     ATRNTFIPVDAFGGININSQLSFSRTHILGADMATSIGGFGLWAEVAAFIPDKDIIMT
                     NDFSAFYPASPVPVTVDSLLLESSKPYVRFVIGGDYNFSNSSYLNFQYMHGFINERGE
                     GNLNDYFFVRYEKKFFNEKLRVAPIGGGFIVTDWNKIKDNYTLLFVPEVAYQATDNIE
                     MSLSAAIIEGKGSGVFGNLNDYDMLMFKLKYSF"
     gene            complement(3991..4524)
                     /locus_tag="VF870_01580"
     CDS             complement(3991..4524)
                     /locus_tag="VF870_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9250897.1"
                     /translation="MNLIRNLCRDGDYRISLLVSFGSFWGLRISDILSLKWHQVYDMD
                     EFELVEKKTQKCRVIKINGQLKKHINDCYQKIKPCSVEESIFISRKGSVYTVQRINVI
                     LKDLKVRYNLKIKNFSSHSLRKCFGREVFCRSDSNAELALVKLSQLFNHSSPSITRRY
                     LGISQKELMETYEILSF"
BASE COUNT         1564 a          749 c          872 g         1457 t
ORIGIN      
        1 agaattgcca ccagtgcaaa gaatcagaat tttgcaggta cggatttctc ttatgatgat
       61 atggaatcaa aacctttttc agcaaaatac acgcctaaat taattgaaac cggagcaaat
      121 gtttatacgc tggaactgac accacaggct caatctgatt attccaagat aattgtaacc
      181 atcaataaaa caaacttcta cccggagacg atggattatt atgacaaagg aaacacaaaa
      241 attaaatctg caaaatatgt gtttagtaag gtaggtaact actggaatgc tcaggaaatt
      301 gaaatgactg actttaaaaa aaatcataag acaaaaatgc aaatgtcaga cgtgaaatat
      361 gatacaggac taaccgatga tgattttacg gtaagaaaat taattcagta ggtactgtta
      421 attgactaaa cctcacacgt tgaaaacgat atcaggatat ttactcaagg atgttttggg
      481 gtggaaaatc aaaggtgaat ttccagacat aaaaaagtca atcacaatat ttgccccgca
      541 tacagcgcac attgatgccc tctatgggaa attaggtata gctcaaatgg gtgtcagata
      601 cttattcctt tcgaaaaagg aacttttctt tttccccatg aatattgtta tgaaaaaatt
      661 tggttcaatg gcggttagag gtgtcaaaga cgaaaatgcg atatataagg ttgttaagat
      721 tttaaatgaa aatgatgaat tacatgtcgt aatttctccc gaagggtcga ttaatagggt
      781 aacaaattgg aataaaggat tttactatat ggcacagaag gctaatgtac ctattgtggt
      841 tacatctctg gactacaaga aaaaagaaat ggaagtcaaa ggtgtcatct atgaccttaa
      901 tgattatgtt actgtaataa agcaaattaa tcagatgtat aatggtgtta caggcaaagt
      961 tcatcagaat ttttcccttt catcagaaac ctgaaatatg aataaatcag ttgcattagt
     1021 attatccagt ggaggttcaa agggtcttgc acagattggc gtttgttacg cacttgaaaa
     1081 gcatgggttt caaatatcct ctattgcggg ttcttctata gggtctgtta ttggcggaac
     1141 ttattcaatg ggtaaactgc cggaatttac tgcatgggta aaatcattgg acagaaaaac
     1201 agtttgggga ttaatggatt tcactatttc aactaacggg ttgttaaaag gagcacgagt
     1261 actcgaaaaa atgaaaacat tcatccctga tatgaacatc gaagagatgg gaataccgtt
     1321 ttctgcaatt gccgttgata tattaaatga aaaagtagag gtctttaatt caggtagttt
     1381 ttatgaagct atcagagcat ctatagcaat cccgactttg atgacaccag taatatccgg
     1441 ggataaaatt ttagttgatg gtggcgtact aagtcctatc ccaattgaat atgtaactcg
     1501 tcagcctgaa gatattcttg tggttgtgaa tttatatggg gacaagaaaa atgatttacc
     1561 tgatacatta tccactgtta acaaacagca taaagttatt cctaataatt tgttaaaaat
     1621 tattaccaaa gccaatgata ctggtgataa gaaaagtatg tggtattatt ctcttttaaa
     1681 agccactacc tctgaaatga ttcataaaat tgcaaaacaa accattgaat tattcaaacc
     1741 tgatattctg attaatatcc catatgattc ttctgataca ttcgatttcc ataaagcaga
     1801 ggaattgata aaaattggag agaatgctgc aaatgaggca atttcaaact atttgaatca
     1861 tgaattatga aatcttcggt agtgatatgt gcttataacg aagataagac aatcagagat
     1921 gtgttggttt cagtgtctga ttgcaaaata tttgatgaaa ttattgttgt caatgatggt
     1981 tctgccgata acaccaaaga aatcataatt gattgtaaga atgaattgga tattattgat
     2041 atccatctgc ctgaaaataa gggaaaaggc tttgccatgg caacaggtat tgaacatgcg
     2101 agtaatgaat tgattgtttt ttgtgatgct gatttatcca atttaaaaag agaacatttt
     2161 ctacaactta ttaaaccaat tattagaagc gatgcaaaaa tggttcttgg tcaaccaact
     2221 gaaaccttga ttaattttca catcaatcct ttcaaagttc tcacaggtca gagagcagta
     2281 ttaagaagtg atgtactgcc aatcgttgaa gatatgaaaa catcaaagtt tggcgtagag
     2341 acccttataa acctttatta tgaggctcaa aacaaaaaag tgaaacacgt caaattaaaa
     2401 ggattaaagc atcccacaaa atttaataaa acaaatctat caggagcaat gaaggcattt
     2461 ctgtttgagg gaattcaaat tgctcttata gtttttaata atttaaactt aattacaaag
     2521 attattagta acagaatcaa taacttataa cgtatgaaac tattaaaact aacttcagtg
     2581 ataattatac ttttagcgaa ctcttatatt gtcagagcgc aagaaagtag ctttaccgca
     2641 ccggaacaag ctgaaagcag gctgaatatc acaggttcgc tattgactga cgaacgattt
     2701 ctgctgactg ataaaaagga ttgggcatgg aatgaaaatc gtcttactct taaattggat
     2761 aaaaaaatca gcaacaggtc aaaattttac agtgaggtct ggcttcgtaa tattggttta
     2821 ccaaatatta cttcatctgc tgatttatat aataagggga tagttgaccc gattaatttt
     2881 gaattaaggg aagcatatgt tcagatattc ggcttccttt caaaaaactt agacattaca
     2941 ataggtcgtc aaagaatagt ctggggtact gctgacaaac ttaatcctac agacaatctg
     3001 aatccttatg atttggagga cattcttgat tttggacggc atcggggttc agatgctgta
     3061 agtcttaact actatataaa caatgatttt tctcttcagg gtgtctacat tcccattttc
     3121 caacctgcaa acatgccaat tggtatttat gcagatgcat tatcacctga aatggattta
     3181 ccacaaggta tggtattgaa cgaattttca gatactatac ttatgccaag gtacaatctg
     3241 gcagaaagtt cgactgctgg cttgaagttt aaaggttttg tcaagggagt cgatttttca
     3301 ttgagctatg tttggggata tgatggatta cctttcgcaa cacgaaacac atttatccct
     3361 gtcgatgcat ttggaggtat aaatataaac tctcagcttt ccttttcaag aactcacatt
     3421 ctgggggctg atatggcaac aagtataggc ggatttggat tgtgggcaga agtggcagct
     3481 tttattcctg ataaagacat tataatgacc aatgattttt cggcatttta tcctgcctca
     3541 cctgttccgg tgactgtgga ctcgttgtta ttggaatcat cgaagccata tgtaagattt
     3601 gtaattggtg gtgattacaa tttctcaaac agttcgtacc tgaatttcca gtatatgcac
     3661 ggttttatta atgaacgggg ggaaggaaac cttaacgact atttttttgt acgatatgaa
     3721 aaaaagttct ttaatgaaaa actaagagtt gcccctatag gtggcggttt tatagtgaca
     3781 gactggaata aaataaaaga taattacacc cttttatttg taccggaggt ggcttatcag
     3841 gcaaccgaca atattgaaat gagtctgtct gctgcaatta ttgaaggtaa agggagtgga
     3901 gttttcggaa acttgaacga ctatgatatg ctaatgttta aactcaaata tagtttctaa
     3961 tccccgacct gattcagaac tctattttta ttaaaaactc agaatttcat aagtttccat
     4021 cagttccttt tgacttatgc caaggtatct ccttgtgatt gaaggacttg aatgattaaa
     4081 aagttgtgac aacttaacta atgccagttc tgcattcgaa tcagaacggc aaaacacttc
     4141 cctgccgaag cattttcgta gtgaatggct gctgaaattt ttaatcttca gattatatct
     4201 gactttcaag tctttaagta tcacattaat tcgctgaact gtgtaaacag accctttcct
     4261 gcttataaaa atagattcct caacagaaca gggtttgatt ttctggtaac agtcattaat
     4321 atgctttttt aactgcccgt taattttaat cacccggcat ttttgagttt tcttttcgac
     4381 caattcgaat tcatccatgt cataaacttg atgccatttt aatgaaagaa tgtccgaaat
     4441 tctaagtccc cagaatgaac caaaggaaac aagcaatgaa atccgataat cgccatcccg
     4501 gcacaggttt cttatcaaat tcattgattg actccattcg aggaaatcgg aagtcgtgtt
     4561 tgaaaatttt gcactcataa tttatttcag ttttgtttat tatctatcga aagtgaatgt
     4621 gatatttccc ggatttcatt at
//
LOCUS       DASWBY010000075         5711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_717334, whole genome shotgun sequence.
ACCESSION   DASWBY010000075 DASWBY010000000
VERSION     DASWBY010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717334"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VF870_01585"
     CDS             <1..710
                     /locus_tag="VF870_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250898.1"
                     /translation="ITELSMLAIRLNEIYNSLSQNPIDFLKISDQFKEHSRYLIKDLG
                     HMLDITTPAVFKTAIEKLNTSIVFPKEEIKIDLSKRIPEDEPFIKSETDEIKEKIIFS
                     EDVDNDEELIQKFEPTILGPIKPLDNLLKKISNNEIDYEELIKFSQIMEDNAVLSRKI
                     GFEILSGMHRIVSKALLLIKNRELMPGKEVIENLRACLIVIVAVVRGKDVDINNYLNK
                     AEEFGNKLATIKSKETY"
     gene            710..1777
                     /locus_tag="VF870_01590"
     CDS             710..1777
                     /locus_tag="VF870_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX9250899.1"
                     /translation="MKLYAVIMAGGVGSRFWPRSKKKTPKQLLKIFGDHTMIEATVNR
                     LSGLIEKENIFVITNQLQRPEVINQLPQVPAENIIEEPFGRNTAACIGLASVIIKAKD
                     PNAITIVLPADHIIKDEEKFKQVLENASKFANDSKGLVTIGITPTRPETGYGYIQIND
                     KEVAENIFQVYTFAEKPNYATALRFVESGDFLWNSGMFIWRADVILDEIKTLMPDLYE
                     GLVAIEKSLSSPNFKEELKSVYAQLKKVSIDYGIMEKSSKVFLTKGSFNWSDVGSWEE
                     VYQLSEKNENGNAAIGKVYTNMVSDSYIYSPDKVTAVIGLDNVIVINHHDTVLICRRD
                     KAQDVKEIVDYLKMNKMDEYL"
     gene            1897..2658
                     /locus_tag="VF870_01595"
     CDS             1897..2658
                     /locus_tag="VF870_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX9250900.1"
                     /translation="MFIVNSPIVETKQVHENIFIQKIYSPVIARLIKPGQFLNIRVSE
                     NTFPLLRRPFSICDVDGDNIFLMFNILGEGTKILSHKHPGDLVDILGPLGNGFNLEGD
                     YKTAIIVAGGLGAAPFPFVTREIKDKKNILSFVGGRTKQDVIEYGFQNLILSSDDGSI
                     GFMGNVVQSLESKIDKIDIASSKVFACGPNAMLRVLKEFTLKHNLDCEVSTECAMACG
                     FGICQGCPIESTSNSDKYLLVCKDGPVFNVKDVVI"
     gene            2655..3572
                     /locus_tag="VF870_01600"
     CDS             2655..3572
                     /locus_tag="VF870_01600"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX9250901.1"
                     /translation="MSKVDLSVKIGSLLLRNPIMLASGTVGYGNEISEFSDLNKLGAI
                     VTKSLSLKPRKGNPPQRIVETPAGMLNAIGLANVGVEEFLKEKIPFLKKYDVPLICNI
                     AASTVEEYVECTRILNSEDTIKAFEINVSCPNVKDGGLQFGNDKNAVGNVTSKVRAVT
                     NKPLIIKLSPNVSYISDFAQVVKDEGGDAVSAINTLVGTAFNIFTRKPKIFNVNGGLS
                     GPAIKPVALAKVLEISRKVDISIIGIGGIMNWKDVIEFMIVGASAVQIGTLNFIDPTA
                     PEKMIKELEDYCIASKVEKLSDITASYII"
     gene            3573..4856
                     /locus_tag="VF870_01605"
     CDS             3573..4856
                     /locus_tag="VF870_01605"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX9250902.1"
                     /translation="MDIQESLNRLFALHTFGVKLGLENIQKFLKDIGNPQGKLKTIHI
                     AGSNGKGSTASFIASILIENGYKVGLYTSPHFVKFNERISINGKYISDDFIANFIDKY
                     QKQIDDLKLTFFEVTTAMAFDYFLFSDVDYAVIETGLGGRLDATNVLNPLACVITSIS
                     LEHTAILGDKLEQIAFEKSEIIKSGSKTFIGLLPEEAIKVIEKKCLSVKSPLSCLEEY
                     IIERDDKIELYTEELEFDDWEVPLIGKHQKYNAALASLCVAKTFDIDDPAIITRGIKN
                     VVKNTKLQGRYEIISEKPRIILDSAHNPEGISCLINQFKKEKNNYNVCSLLFGAMSDK
                     NIDEMLLLVKDSFNAIYFYGINYERAASIALLSERAAKHNIKFSVVDNLGEFIKEQSN
                     GDKLNCLVIAGSMYLLGEIKSILPKLNLDNINVSM"
     gene            4987..>5711
                     /gene="rho"
                     /locus_tag="VF870_01610"
     CDS             4987..>5711
                     /gene="rho"
                     /locus_tag="VF870_01610"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX9250903.1"
                     /translation="MPMDISELKSKKIVELNELAKDLNIAGYSDLRKQELIFKILEAQ
                     SVKDGLTFSKGVLEVLPDGYGFLRSADYNYLPSPDDIYVSPSQIKKFSLRTGDFVSGQ
                     VRPPKEGERFFALLRVEAVNGLSPDHIRNRTLFDNLTPVYPTKKLLLESVPGEYSTRI
                     MDMLAPIGKGQRGLIVSPPKSGKTVLLQKIANSITRNHPEVKLLILLIDERPEEVTDM
                     ERSVKAEVISSTFDEPADRHVQVA"
BASE COUNT         2013 a          832 c         1092 g         1774 t
ORIGIN      
        1 agataactga attgtctatg ctggctattc ggttaaatga gatttataat tcactctctc
       61 aaaatccaat tgattttctt aaaatatcag atcagtttaa agagcatagt agatatctca
      121 ttaaagatct tggccacatg cttgatatta caacgcctgc tgtatttaaa acagcaattg
      181 aaaaacttaa tacttcaatt gttttcccaa aagaagaaat taaaattgat ttatctaaac
      241 gcattcctga agatgaacca tttataaaat ctgagactga tgagattaaa gaaaaaatta
      301 tcttcagtga agatgttgat aatgatgaag agttgattca gaaatttgaa ccgacaattc
      361 ttggacctat aaaaccgctg gataatcttc ttaagaaaat atccaacaac gaaattgatt
      421 atgaagagct gattaagttt tctcaaatca tggaagacaa tgcagtgtta tcacgcaaaa
      481 taggatttga aattctatct ggtatgcatc ggattgtttc taaagcatta ctattaatta
      541 aaaacaggga attgatgccc ggtaaagaag tgatcgaaaa tttacgtgcc tgcttaattg
      601 ttatagttgc agtagtgcga ggcaaggatg tggatataaa taattattta aacaaagcag
      661 aagagtttgg aaataaatta gcaacaataa aaagtaagga aacttattga tgaaattata
      721 tgcagtaata atggcgggcg gggttggctc acgattttgg ccgagaagca agaaaaaaac
      781 accaaaacaa ttattgaaaa tatttggcga tcatacgatg attgaggcta ctgtaaatcg
      841 tttatcaggt ttaatagaaa aagaaaatat ttttgttatt acaaaccagt tacagcgacc
      901 tgaagttata aatcaattac ctcaggttcc tgcagaaaat ataattgaag aaccatttgg
      961 aagaaatact gccgcttgta ttggtttagc ttcagtaatt attaaagcta aagatccaaa
     1021 cgcaataaca atagtgcttc ctgcggatca tattataaaa gatgaagaga agtttaaaca
     1081 agttttagaa aacgcatcaa agtttgcaaa cgactctaag ggattagtta caatcggaat
     1141 tacaccgaca agaccagaaa ccggttatgg ctatattcag ataaatgata aagaagttgc
     1201 tgaaaatatt tttcaggttt atacttttgc cgaaaaacct aactatgcaa cggctttaag
     1261 atttgttgaa agcggcgatt ttctttggaa tagcggaatg tttatctggc gggcagatgt
     1321 tattctggat gagattaaaa ctcttatgcc ggatttgtat gaaggtttgg ttgcaattga
     1381 gaaaagttta tcgagtccca attttaaaga agagttaaaa tctgtttatg ctcagttaaa
     1441 aaaagtttct atagattatg gtatcatgga aaaatcatca aaagtatttc ttaccaaagg
     1501 ttcgtttaac tggagcgatg ttggaagctg ggaagaagtt tatcagttat cagaaaaaaa
     1561 tgaaaatggt aatgctgcca tcggaaaagt ttatactaac atggtctccg attcttatat
     1621 ctattcaccg gataaagtta ctgctgtaat tggtctagat aatgttattg taataaatca
     1681 tcacgacaca gttttgattt gtagaagaga taaagctcag gatgttaaag aaattgtaga
     1741 ttatttgaag atgaataaaa tggatgaata tttatagttt attcggaaat ctcttcagaa
     1801 gataatagtt ttgtaaggaa gattttaatt tatttacctt aaccttcttt tgtgtctgta
     1861 tccaaaacct tgtaattgat tttaagagga atcagattgt ttattgtaaa ttctccgatt
     1921 gttgaaacta agcaagttca cgaaaatatt tttatccaga aaatttactc accagtaatt
     1981 gcaaggttaa taaaaccagg acaatttcta aacattcgtg tatctgaaaa tacattccct
     2041 ttgttaagaa ggccattcag catttgcgat gttgatggcg ataatatttt tctgatgttt
     2101 aatattttag gtgaggggac aaaaatactt tctcataaac atcccggaga tttggtagat
     2161 attcttggtc cgcttggaaa tggatttaat cttgaaggag attataaaac agcaattatt
     2221 gttgcaggcg gattgggtgc agcaccattt ccatttgtaa caagagaaat aaaagataaa
     2281 aaaaatattc tgtcatttgt cggcggcaga accaaacagg atgttattga atacggattt
     2341 caaaacttga tcttatcctc tgatgatggc tcgattggtt ttatgggaaa tgttgttcaa
     2401 tccttagaaa gtaaaattga taaaattgat atcgcttcat ccaaagtttt tgcctgcgga
     2461 ccaaatgcga tgctgagagt gttaaaagaa tttaccctta aacataatct tgattgtgaa
     2521 gtatcaacag aatgtgcaat ggcctgcgga tttggaattt gccagggctg ccctattgaa
     2581 tcaacatcaa actcagataa atatctttta gtttgcaaag atggtcccgt atttaatgtt
     2641 aaggatgttg tgatatgagc aaagtagatt tatcggtaaa gatcggctca ttactactgc
     2701 gtaatccaat aatgctcgca tccggtacag ttggatatgg aaatgaaatt tcagagttct
     2761 ctgatctaaa taagcttggt gcaattgtta caaaatcttt aagtctaaaa ccaagaaaag
     2821 gtaatccacc gcagcgtatt gttgaaactc ctgcaggaat gttaaatgca attggtttgg
     2881 ctaatgttgg agttgaagaa tttttaaaag aaaaaatccc ttttctaaaa aagtatgatg
     2941 tgcctctaat ttgtaatatt gctgcaagca ctgttgaaga gtacgttgag tgcacaagaa
     3001 ttcttaattc tgaagacaca ataaaagcat ttgagattaa tgtttcctgt cccaatgtaa
     3061 aagatggcgg attgcaattc ggcaatgata aaaatgcagt tggcaatgtt acttcaaaag
     3121 tacgtgcagt aactaataaa cctctcataa taaaattatc accgaatgtt tcctacattt
     3181 cagattttgc gcaagttgtt aaagacgagg gaggcgatgc agtttctgca attaacacac
     3241 tagttggaac agcttttaat atatttacga ggaaaccaaa aatatttaat gtaaatggcg
     3301 gactttcagg tccagctatt aaaccggttg ccttagctaa ggttttagaa atatcacgca
     3361 aagttgatat ttctattatt ggtattggcg gaattatgaa ctggaaagat gtgatagagt
     3421 ttatgattgt tggagcttcg gcagtgcaga ttggtacatt aaattttatt gatccaactg
     3481 ctccggaaaa aatgattaaa gaattagaag attattgtat cgcaagcaaa gttgaaaaat
     3541 tatcagacat aacagcatcc tatattattt aaatggatat tcaagaatca ttaaatagat
     3601 tgtttgcact tcacactttt ggagttaaac ttggattaga aaatatccag aagtttttaa
     3661 aggatattgg aaatccacag ggtaaattaa aaactatcca cattgccgga tcaaacggaa
     3721 agggcagtac cgcatctttt attgcaagca tacttataga aaatgggtac aaggttggat
     3781 tatacacttc accccatttt gtaaagttta acgaaagaat ttctataaat gggaagtata
     3841 tctctgatga ttttattgca aactttatcg ataaatatca aaaacagatt gacgacttaa
     3901 aactgacttt ctttgaagtt acaacggcaa tggcttttga ttattttctt ttttctgatg
     3961 ttgattatgc tgttatagaa acaggattag gcggaaggtt agatgcaact aatgttctca
     4021 atccgcttgc ttgcgtaatt acatctataa gtttggagca tactgctatt cttggagata
     4081 aacttgagca aatcgcattt gaaaaaagtg aaataataaa gagcggctca aaaaccttta
     4141 tcggattgtt acccgaagag gctatcaaag taattgaaaa aaaatgcctg tcagtaaaat
     4201 cacctctatc ttgtttggag gagtacatta ttgaaagaga tgacaagata gagttatata
     4261 ccgaagagtt agagtttgat gattgggaag ttcctcttat tggtaagcat cagaaatata
     4321 atgctgcatt agcttcactt tgcgtagcta aaacttttga tattgatgat ccagcaatta
     4381 tcacacgcgg aattaaaaac gtagttaaga atacaaagct tcaaggcaga tatgaaatta
     4441 tttcagaaaa accccgaatc attttagatt ctgcacataa tccagaggga attagctgtt
     4501 taattaacca gtttaaaaaa gagaaaaaca attacaatgt ttgctcatta ttgttcggtg
     4561 ctatgagcga taagaatatt gatgagatgc tgttattagt taaagatagt tttaatgcga
     4621 tttactttta tggaattaat tatgaacgtg cagcaagcat tgccttatta agtgaacgtg
     4681 ctgcaaagca taatataaaa ttttcggttg ttgataatct tggggagttt attaaagaac
     4741 aatcaaatgg agataaactt aactgtctgg taattgcagg gagtatgtac ttgctgggtg
     4801 aaattaaatc aatattaccc aaattaaatc ttgacaatat aaatgtaagt atgtaagttt
     4861 ggttaagacc tcgagttcct ttcagacaat ttaagaaata ccacgagagc ttatcaggat
     4921 ttaaccaatt tgataacaat tttaatctaa aaaatcattc taacaaacaa aatataggta
     4981 ccccaaatgc cgatggacat ttctgaactc aaatcaaaga agattgttga attaaatgaa
     5041 cttgcaaagg atttaaatat tgcgggttat agtgatctaa gaaaacagga attaatcttc
     5101 aagatattgg aagctcagag cgttaaagat ggattaacat tttcgaaggg agttttagaa
     5161 gtacttccgg atggatacgg atttttacga tctgcagatt ataattactt gccttcacca
     5221 gatgatatat atgtttcacc ttcacaaata aaaaagttca gccttagaac aggtgatttt
     5281 gtaagcggac aagtaagacc gccaaaggaa ggagaaagat tctttgcact gcttagagta
     5341 gaggcggtaa atggtctgtc tcccgatcat atccgaaacc gcactttgtt cgacaacctt
     5401 acgccagttt atccaactaa aaaattatta cttgagtcag tgcctggtga atattcaacc
     5461 agaataatgg acatgctcgc acccataggt aaagggcaaa gaggtttaat agtgtcgcca
     5521 cctaaaagcg gtaaaactgt tttactccag aaaatagcaa actcaattac acgtaatcat
     5581 ccagaagtta aacttttaat cttgcttata gatgaaagac ctgaagaagt aaccgatatg
     5641 gaacgatctg taaaagcaga agttataagt tcaacttttg atgagcctgc tgacagacac
     5701 gtacaggttg c
//
LOCUS       DASWBY010000076         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_40656, whole genome shotgun sequence.
ACCESSION   DASWBY010000076 DASWBY010000000
VERSION     DASWBY010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40656"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VF870_01615"
     CDS             complement(<1..489)
                     /locus_tag="VF870_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250904.1"
                     /translation="MSEEEKTYKKLLDDLKNLPKIDAPKNFETELLRKINSSKEDKRE
                     SFWDKLLSPGKLTPAAVAIASAGIIFFVVDINSEKVEDPLNIAPKLREDIVVIQSLDE
                     IPVEQLKKSARVKEESVDRNEGMIESKEVETPVQPNNVFEEQSEMKSKGVDQSLKDEM
                     VSG"
     gene            complement(590..1177)
                     /locus_tag="VF870_01620"
     CDS             complement(590..1177)
                     /locus_tag="VF870_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9250905.1"
                     /translation="MTDRNLSEFTDEELILEFQSNGTEKAFEILVQRFKNPLTNYVYR
                     FLGDYDSCVDVVQDTFVKVYRYKDNYSSVAKFSTWIYTIAGNLARTEYQRKRRRNFFS
                     INSFGEEDKSFDIPDHSARPDVAADMNMKNEIIQKALLKVRDSYREAVILRDVQELSY
                     EEIAEIMKIEVGTVKSRINRGRTELQKYLKDIYND"
     gene            complement(1270..2289)
                     /gene="holA"
                     /locus_tag="VF870_01625"
     CDS             complement(1270..2289)
                     /gene="holA"
                     /locus_tag="VF870_01625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX9250906.1"
                     /translation="MAKSKAPAIQDIEKQLKQKKILPVYYLFGEDTYSVELTFEAIEK
                     AVQPFITSDFDKEVLYGENQSFAGIIGLASTFPFGSEKKLIIVKQAEKLKEKKEKKEI
                     VSYFESPTDFTVLVFLHEGTITNPASEPYKTLAAQGFLFEAKELKGKSLIDWLISTVE
                     KNGKTISYDNAQLLTDISGENRNTLESQLEKIFIYIGDNKEITIESIRGLSTSLKQYT
                     IFDLQNALGKKNKSSALKVAFNLLKNGMEPIQIIAMLNKYFTSLARLTELTAANTNEF
                     QVARIMSTHPFYLKDYHAARRMYSDKQLTTAFSALLKADLSIKTTSLDDYTLISLLIA
                     EIIPD"
     gene            complement(2472..3041)
                     /locus_tag="VF870_01630"
     CDS             complement(2472..3041)
                     /locus_tag="VF870_01630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9250907.1"
                     /translation="MKKLFFAAAIIGLILSSNIFSQKKETKKMSDSTTVAIIKTNMGT
                     IELELFAKETPKTVENFVGLANKGYYNGVIFHRVIDGFMIQGGDPTGTGRGGESFWGK
                     KFNDEIVKDLVFDKEGILAMANAGPNTNGSQFFITLAPTKWLNGNHTIFGKVINGMDV
                     VKAIGKVQKGPQDRPVKDVVMESVTIEKR"
     gene            complement(3050..>3625)
                     /locus_tag="VF870_01635"
     CDS             complement(3050..>3625)
                     /locus_tag="VF870_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX9250908.1"
                     /translation="NSKLGWMIILGTIPIVVIGLLFKDMIEGALTKNLYVISGSLIAL
                     AIILAVAEKTAKFKKNIEDITLLDSILIGFAQAVALIPGSSRSGTTITGGLFLGLKRD
                     VAARFSFLLSVPAVFASGMLQLKESLAYINYDMAVSMIVATIVSGISGYLAIYFLLKF
                     LKKNTTFVFIFYRIGLGVLILFLLYSNIIKP"
BASE COUNT         1099 a          682 c          522 g         1322 t
ORIGIN      
        1 gccagaaacc atctcatcct ttaatgattg atcgactcct ttggatttca tttcagattg
       61 ttcttcaaaa acattattgg gctgtacagg tgtctcaact tcttttgatt cgatcatccc
      121 ctcattgcga tctacagatt cttctttaac tcttgctgat ttttttagtt gctcaacggg
      181 tatttcatct aatgattgaa taactactat atcttcacgc aattttggtg cgatattaag
      241 aggatcttca actttttctg aattaatatc aacaacaaaa aagattattc ctgccgatgc
      301 tatagccact gcggcaggtg ttaattttcc gggcgacagt agtttatccc aaaaactttc
      361 tctcttatct tcttttgatg aatttatttt tcttaaaagt tctgtttcaa aatttttggg
      421 tgcatctatc ttaggaagat tttttagatc atctaataat tttttgtatg ttttttcttc
      481 ttcgctcatt ttattatact taaatcattg ttccgtcacc ctgaacttgt ttcagggtct
      541 tcataactaa tatgagatgc tgaaataaat tcagcatgac tttttaacct taatcattat
      601 aaatatcttt taaatatttc tgcagctctg ttctgccgcg atttatcctt gattttacag
      661 tgccgacttc aattttcatt atctctgcaa tttcttcata agataactcc tgaacatctc
      721 taagtataac agcctcacga tatgagtcac gcacttttaa taatgctttc tgaataattt
      781 catttttcat attcatatct gctgcaacat ccggacgagc agagtgatca ggaatatcaa
      841 aagatttatc ttcttctcca aaactgttta tcgagaaaaa atttcttctt cgttttctct
      901 ggtattctgt tcgtgccaga ttacccgcaa ttgtataaat ccatgtagaa aactttgcta
      961 ctgaactgta attatcctta tatcgataaa cttttacaaa agtatcctgc acaacatcaa
     1021 cacaagaatc ataatcacca agaaacctgt aaacataatt tgttaacgga ttcttaaatc
     1081 tctgaactaa aatttcaaaa gctttttcag taccattact ctgaaattca agaatcagct
     1141 cttcgtcagt aaattcgcta agatttctat ccgtcaacgg tattatactt tattagttgg
     1201 atgtttgttt caattatcag acaaaattta caaaaacttt agaaaagctt ttgtggattt
     1261 ttttcttatt taatcgggta ttatttctgc aattagtaga cttataagcg tataatcatc
     1321 aagagatgtg gttttgattg ataaatcagc ttttaatagt gccgaaaaag ctgtggttaa
     1381 ctgtttatca gaatacattc ttctagcggc atgataatct ttaagataaa atggatgagt
     1441 actcataatt cttgcaacct ggaattcgtt tgtattagcc gcagttaatt cagttaacct
     1501 tgcaagacta gtgaagtact tatttagcat tgcaattatc tgaattggtt ccattccatt
     1561 tttaagaaga ttaaaagcaa cttttagtgc tgaagattta ttctttttac cgagtgcatt
     1621 ttgtagatca aaaattgtgt attgttttaa tgaagtagat aaaccgcgaa tgctttcaat
     1681 ggtaatttct ttattgtccc caatgtaaat aaatattttt tcaagctgcg attctaaagt
     1741 gtttcggttt tcaccggaaa tatctgtgag cagctgagca ttatcatacg aaatagtttt
     1801 accatttttt tctacagtag aaattaacca atcaattaaa ctcttgcctt ttaattcttt
     1861 ggcttcgaat agaaatcctt gtgcggccag cgttttataa ggttcggatg cgggatttgt
     1921 aattgttcct tcgtgaagga aaacaagaac cgtaaaatca gtgggtgatt caaaataact
     1981 aactatctct tttttttctt ttttctcttt tagtttctcg gcttgcttaa ctataataag
     2041 ttttttttct gaaccaaaag gaaatgtaga agctaacccg atgataccgg caaatgattg
     2101 gttttcaccg tacaatactt ctttatcaaa atcagaggta ataaatggct gaactgcttt
     2161 ttcaattgct tcaaaagtaa gctccacact atatgtatct tcgccgaaca aatagtaaac
     2221 aggtaaaatc tttttctgtt ttaattgctt ttcaatatcc tgaattgccg gtgctttact
     2281 tttagccatt gttaactttt aaattgaaga ggctgtatca aaaggctcgg tttgtcaccc
     2341 tgaactcgtt tcagggtctg aatttaattc aaaatcgcac cgttagattc cgaaataaac
     2401 ctgtctgcag acaggtaaga ttcggaatga cacttttgag acagcctcat taataattca
     2461 atataatcta tttatctttt ttctatagta acactttcca tcactacatc tttaaccgga
     2521 cgatcttgtg gtcctttttg aactttaccg atcgctttaa ctacatccat tccgttaata
     2581 acttttccaa aaattgtatg atttccattt aaccattttg ttggtgctaa tgttataaaa
     2641 aactggcttc catttgtgtt aggaccggca tttgccattg ccagaattcc ttctttatca
     2701 aacacaagat ctttaacaat ttcatcatta aactttttgc cccaaaaact ttcaccgcct
     2761 cgtcctgttc ctgttggatc accgccctga atcataaatc catcaatcac tctatgaaag
     2821 attacgccgt tatagtaacc cttgtttgcc agtccaacaa aattttctac tgtttttgga
     2881 gtttctttag caaataattc taattctata gtgcccatat tggttttgat aatcgcaacc
     2941 gttgtgctgt cactcatttt ctttgtttcc tttttttgtg aaaaaatatt cgatgataaa
     3001 attaatccaa ttattgccgc agcaaaaaat agttttttca tttaacctcc tatggtttta
     3061 ttatgtttga ataaagtaaa aataaaatta aaacgccgag cccaattctg taaaagataa
     3121 aaacaaatgt tgtattcttt tttaaaaact tcagaagaaa ataaattgcc agataaccgc
     3181 ttataccgga tacaattgtt gctacaatca tactaacggc catatcataa tttatatatg
     3241 ccaaagattc ctttaactga agcattccgc ttgcaaaaac tgccggaaca cttaacagaa
     3301 atgaaaatct tgcggccaca tctcttttta accctaaaaa taatccgcca gttattgtcg
     3361 tacccgatct tgaggaaccg ggaatcaatg caaccgcttg tgcaaatcca attaaaattg
     3421 aatcaagcaa agtaatatcc tcaatatttt tcttgaactt tgcggttttt tctgcaacag
     3481 caagaatgat tgcaagcgca attaaactac cgcttattac atacagattt tttgttaatg
     3541 ccccttcaat catatcttta aataacaggc caataacaac aattggaatt gtaccaagta
     3601 taatcatcca gccaagtttt gaatt
//
LOCUS       DASWBY010000077         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_153511, whole genome shotgun sequence.
ACCESSION   DASWBY010000077 DASWBY010000000
VERSION     DASWBY010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153511"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VF870_01640"
     CDS             complement(<1..833)
                     /locus_tag="VF870_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9250909.1"
                     /translation="MFLKNHNKPALILKDHVIDYELLINKVHAFSTLLPSQNLSKVAI
                     FSENRFEWVYAFYSVWKKKAIAVPIDFMSSAEDVSFILNDCKPEVIFYSNGTKEACDK
                     ALALLNHKIEKINLDEITIEETITKDTFPDPDPNETAVIIYTSGTTGSPKGVMLSYDN
                     LMVNIEAVTSDVQIYKSEDRVMILLPLHHIFPLVGTMVAPLSVGGTVVFSPSMASEDI
                     MATLQHGITIIIGVPRLYNLIRKGIRDKINKSGVAKLLFKIAEKKNSLTFSRKLFGSV
                     Q"
     gene            1025..1258
                     /locus_tag="VF870_01645"
     CDS             1025..1258
                     /locus_tag="VF870_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250910.1"
                     /translation="MEYTREQLIAQKKIQLKVIDLALGILVGMLYGYAIDNLMVGVFI
                     GIGVGLFKWINSIPFWGVFALTIVYWFIIIFIK"
     gene            1272..1661
                     /locus_tag="VF870_01650"
     CDS             1272..1661
                     /locus_tag="VF870_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9250911.1"
                     /translation="MSEKQKPEIGSITWCDLTVPNAEEVKDFYAKVVGWQPEGLSMGD
                     YDDFTMIAPESGKVAAGVCHNRGSNAKLPPQWLIYITVEDVDKSAEYCIQLGGKVIVD
                     PKNMPGYGRYCVIQDPAGAVCALFKPE"
     gene            1724..2620
                     /locus_tag="VF870_01655"
     CDS             1724..2620
                     /locus_tag="VF870_01655"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9250912.1"
                     /translation="MFRNRLMTFAIIILLLIACKNDDSNSTQAPDPTPQITKSPKRGL
                     AYDLKNAADLDTLKSGVSWWYNWYHSTSAQNNYYSTYGMEFIPMLWGGNTSTNDMTAV
                     KSFILTHPEIKYLLVMNEPNLVDQANRTPVQAAADWIKYEQVVADLAAQQRTIEIVGP
                     AMNWGTMTNYSDPVVWLDDFYAAYKSANGGRIPKIDYLAFHWYDYGLAAQLDRLQKYG
                     KKIWITEMANWNPQINSYQKQAEQMEEMVNICETRSDVFRYAWFIGRGSLPDSKFTYL
                     IDQNPEQLNDLGKLYLSLPITK"
BASE COUNT          920 a          450 c          507 g          899 t
ORIGIN      
        1 ctttgaacag aaccaaaaag ttttcttgaa aaagttaacg agtttttctt ttcagcaatt
       61 ttaaaaagca gttttgcaac tccgctttta ttaatcttat cacgaatccc ctttctgatt
      121 agattatata aacgcgggac accaattata atcgtaattc catgctgcaa tgttgccata
      181 atatcttcgg aagccattga aggggaaaag accacagtac cgccaacact taagggggcg
      241 accattgtac caactaaagg gaaaatatga tgcagcggta aaagtatcat aacgcgatct
      301 tcgctcttat aaatttgcac atctgatgta actgcttcaa tattaaccat caggttatca
      361 taagaaagca taactccttt tggcgagccc gttgttcctg aagtataaat tattaccgcg
      421 gtttcatttg ggtctggatc gggaaaggtg tctttagtaa tcgtttcttc aattgttatt
      481 tcatcaagat ttattttttc aattttatga tttaacagag caagggcttt gtcacatgct
      541 tcttttgttc catttgaata aaatatcact tcaggtttgc aatcgtttag aataaaagaa
      601 acatcttcag cagatgacat aaaatctatg ggaactgcga tagctttttt cttccacaca
      661 gaataaaaag catacaccca ttcaaatcta ttctcagaaa agattgcaac ttttgaaaga
      721 ttttgagatg gaagcagggt tgaaaaagcg tgtactttat tgattaacaa ttcgtaatca
      781 attacatgat cttttaagat cagtgctggt ttgttatggt tctttaaaaa caatctttta
      841 ccaataattt aatgaagtga taattcaaaa atattaaaat aaatggttaa aaactttatt
      901 gataaccctc attgaagttg tactatgggc caaataaatt atacccaatg ttaaaatatc
      961 ttgctcggca attgtattct tcaaatattt ttcttaagta ttatctatat catttgagga
     1021 aattatggaa tacacccgcg aacaattaat tgctcaaaaa aaaatacaac taaaagttat
     1081 cgatttagct ctgggaatat tagttggaat gctttacgga tatgcgatag ataatttaat
     1141 ggttggagta tttattggca tcggtgtggg attattcaaa tggataaaca gtattccatt
     1201 ttggggagtt tttgcgctta ctattgttta ctggtttata ataattttta ttaaataatt
     1261 ttggcagcac tatgagtgag aaacaaaaac ctgaaatagg ttctataacc tggtgtgatc
     1321 ttaccgttcc aaatgctgaa gaagtaaaag atttttatgc aaaggttgtt ggatggcagc
     1381 cggaaggatt atcaatgggt gattatgatg actttacaat gattgccccc gaaagcggta
     1441 aagttgcagc cggagtttgc cataaccgtg gatcaaatgc aaaacttcct ccgcagtggt
     1501 taatttatat aacagttgaa gatgttgata aaagtgcgga atactgcatt caacttggcg
     1561 gaaaagttat tgttgatccc aaaaatatgc cgggttatgg aagatattgt gtaattcagg
     1621 atcctgcagg tgcagtttgt gctttgttca aacccgaatg attttaagtg tattcttttc
     1681 aacagaaatt tttaaaatat gataaataag gagagtataa attttgttta gaaatagact
     1741 aatgactttt gcaataataa ttcttttgtt aatagcctgt aaaaatgatg actcaaattc
     1801 aactcaagcg cctgacccaa cgccgcagat tacaaaaagt cccaaacggg gacttgctta
     1861 cgacttaaag aatgccgctg atcttgatac actcaaaagc ggagtttcat ggtggtataa
     1921 ttggtatcat agcacaagcg cccaaaataa ttattactca acttacggaa tggaatttat
     1981 cccgatgtta tggggcggca atacaagtac aaatgatatg acagctgtaa aaagttttat
     2041 tcttacacat ccggagataa aatatttact tgtgatgaat gaacctaatc tcgttgatca
     2101 ggcaaataga actcctgttc aggcagcagc agactggatt aaatatgagc aagtagttgc
     2161 tgatcttgct gcacaacagc gtacaattga aattgttggc cctgcaatga attggggaac
     2221 aatgacaaat tattctgatc ctgttgtttg gcttgatgat ttttatgccg cttataaatc
     2281 tgcaaacgga ggtagaattc caaaaattga ttaccttgca ttccattggt acgattatgg
     2341 tcttgccgct cagcttgata gattacaaaa gtatggcaaa aaaatctgga taaccgaaat
     2401 ggcaaattgg aatcctcaga taaactctta ccaaaaacaa gccgaacaaa tggaggagat
     2461 ggtaaacatt tgtgaaaccc gatctgatgt atttagatat gcctggttta ttggcagagg
     2521 aagtctgcct gatagtaaat tcacttattt gattgatcag aatcccgaac aattaaatga
     2581 tttgggaaaa ctttatctca gtttacctat tactaagtaa ataattaatt atttacctga
     2641 aaatatttta atatgatttg tattaaagcg caggaaagga ttcctgcgat actaatatca
     2701 cttacacaat ccaacttcct ttaagcattt tttaattcgc tcgtaagttt ttttaatatc
     2761 attatccagt ccaatt
//
LOCUS       DASWBY010000078         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_3238, whole genome shotgun sequence.
ACCESSION   DASWBY010000078 DASWBY010000000
VERSION     DASWBY010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3238"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..375)
                     /locus_tag="VF870_01660"
     CDS             complement(4..375)
                     /locus_tag="VF870_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX9250913.1"
                     /translation="MSAFLILEVMKRYNKHIFICENKRPEGHPRGCCSEKGSVEIREY
                     FKLRVKELGLNSEVRANAAGCLDACEFGVSVVVYPEQIWYGAVTKNDVDEIIQSHIIN
                     NNPVERLKITHPRYNKDASKA"
     gene            complement(397..470)
                     /locus_tag="VF870_01665"
     tRNA            complement(397..470)
                     /locus_tag="VF870_01665"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Glu,seq:ctc)
     gene            complement(550..622)
                     /locus_tag="VF870_01670"
     tRNA            complement(550..622)
                     /locus_tag="VF870_01670"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(587..589),aa:Gln,seq:ctg)
     gene            756..1601
                     /locus_tag="VF870_01675"
     CDS             756..1601
                     /locus_tag="VF870_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX9250914.1"
                     /translation="MKKIFILYIVISSTLFAQSFNYTESIGKFKKASSFYITANGLIY
                     VSDSGSDELILLDTLGNTLKTFGGYGWDQNSFDDPADVFADPLTIYVADKNNHKIKRF
                     DKNLNFIYALYTRDSDIQEEQFGYPLSCATSNQGDLYFIDSDNKRIMKFDIFGNFIMN
                     FGGFDAGNYQLSKPTQLAVSSSNNIFVIDGSDIVIFDHFGNGIRKINLENKIKSIRIL
                     FDQLVICVDNKIYHTYLKSPDSVLKEIPITGYDIDKQIVSALILNTKLYVLTSDELLI
                     YNKNN"
     gene            2078..2596
                     /locus_tag="VF870_01680"
     CDS             2078..2596
                     /locus_tag="VF870_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250915.1"
                     /translation="MNFKKYLPTLVCGFGAAVLATVPGIKNFSCCLIVPGAAAISILL
                     DKKINNYNEKVVLGKSLGFGFLTGLFATIFITAFEMLVTYFTRSNDFIQSLPQTEILM
                     RQWNLGPLVDESLKLIKNVSKEIQKSGFSFLYLLMILSSNFIINSIFGMIGGALGMSL
                     ANRKSREDDHQV"
     gene            2593..2892
                     /locus_tag="VF870_01685"
     CDS             2593..2892
                     /locus_tag="VF870_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250916.1"
                     /translation="MITAFIFFVHLIFGLVIFTKKWQDENLTAGFLNITLILILFAVG
                     WAITGIVAKLVMEPKGFGMQFDRDTFSLTLLTIIEFFFYKFYYQEDPIAADKEKQ"
BASE COUNT          920 a          470 c          465 g         1052 t
ORIGIN      
        1 ttactaagct ttgcttgcat ctttattata tctcggatgg gttattttta atctttcaac
       61 gggattatta ttaatgatgt gggattgaat aatttcatca acatcatttt tagttacagc
      121 accataccaa atttgttcgg gatacacaac aactgaaacc ccgaattcac aagcatccaa
      181 gcatcctgct gcatttgctc taacttcaga gtttaatcct aattctttca ctcgtaattt
      241 aaagtattca cgtatttcaa cacttccctt ttcagaacaa caacctcgtg gatgtccttc
      301 aggcctttta ttttcacaaa taaaaatgtg tttattatat cttttcatta cttctaaaat
      361 aagaaaagcg gacatcaagt ccgctaattt attttgtagc gcgtacggga ttcgaacccg
      421 tgatccccgc cttgagaggg cggtgtccta agccactaga cgaacgcgcc aatgtaaaat
      481 gaaatttaca atgtacaatt aaaaatgccc taattgtaaa ttctaaattt ttcattgtac
      541 attgatttgt tgtcccgtca ggtttcgaac ctgaactctt ctgatccaga gtcagacgtg
      601 ttgccaatta caccacggga caatttatta cttatttttg atgccaaata tatctgaatt
      661 caattctaaa tgcaatcaga tttaagatgc cgaaattact tgagattggt aaaaatctta
      721 acttaatttg agctaacaat taagatttat tcagaatgaa aaagatattt atactatata
      781 tagtaatttc atcaacattg tttgctcaaa gttttaatta taccgaatcg attggaaagt
      841 ttaaaaaagc ttcctccttt tatattactg caaacggatt aatttatgtt tcggattccg
      901 gaagtgatga acttatttta ctcgacacat taggaaatac tttaaaaact tttggtggtt
      961 atggttggga tcaaaattct tttgatgatc cggccgatgt ttttgcggat cccctgacaa
     1021 tatatgttgc agataaaaat aatcataaaa taaaaagatt tgataaaaat cttaatttca
     1081 tttatgcact ttatactcgc gacagtgaca ttcaggaaga acaatttggt tatccgttaa
     1141 gttgtgcaac ctctaaccag ggcgatctat attttatcga ttctgataat aagcggataa
     1201 tgaagtttga tatctttgga aattttataa tgaattttgg cggcttcgat gccggcaatt
     1261 atcagctttc aaaaccaact caacttgcag tctcatcatc caacaatatt tttgttattg
     1321 atggttctga tattgtaatc tttgatcatt tcgggaatgg aatcaggaaa ataaatctgg
     1381 aaaataaaat taagagtata agaatactct ttgatcaatt agttatttgt gttgataata
     1441 agatttatca cacttattta aaatcccctg attcagttct taaggagatt ccaattacag
     1501 gatatgatat cgataaacaa attgtatcag cgctaatact aaacacaaaa ctttatgtcc
     1561 ttacttcaga tgagttgctg atttataata aaaataattg agattgattt gttaaatcac
     1621 ctgattattc aaagaagatt cttgcgtttt ttaatattat ttattgctgc tttttgggtt
     1681 attggaataa tcagtccctg ttttaatttt gattttactc acgctgctta tccatttctg
     1741 aaacaatgct attcaactgt atgtcaccag aataatgcaa aatcttttac ttgtaataat
     1801 attttctttt tagtttgtgc aagatgcacg gggatatacg tcgggacttt aatttctgcc
     1861 ttcatactta tgtttaattc taaacaattt atctttaaaa ctaaatattt gataatgctt
     1921 tcactgccaa tgctattgga tgttatttta ttgaacctgg gtatttataa ttataatcgt
     1981 ttaatatcag cgttcacagg tttacttttc ggttcctcag tatttctata tattttaagt
     2041 gcaattgaaa atttactttt taacaaacca aacaataatg aactttaaaa aatatcttcc
     2101 aaccttagtt tgcggatttg gtgctgctgt tcttgcaaca gtaccgggaa taaaaaactt
     2161 cagctgttgc cttatagttc cgggtgccgc tgcaatatct attctgcttg ataaaaaaat
     2221 caataattac aatgaaaaag ttgttttagg aaaatcatta ggatttggtt ttttaacagg
     2281 attatttgca actattttca taacggcgtt tgagatgctt gtaacttatt ttacaaggtc
     2341 aaatgatttt atccaatcgc ttccacaaac agaaatatta atgcgccaat ggaatttagg
     2401 accattggtc gatgaatctt tgaagttgat aaaaaatgtt tcaaaagaga ttcagaagag
     2461 cggtttttca tttctttatc ttctaatgat tctttcaagt aactttatta ttaattcaat
     2521 ttttggaatg attggtggtg cacttggtat gagcctggct aaccgaaaat cgcgggagga
     2581 cgatcatcaa gtatgattac tgcatttatt tttttcgtcc atctgatatt tggattagta
     2641 atctttacaa agaaatggca agatgaaaat ctaactgccg gatttttgaa catcacacta
     2701 attttaatat tatttgcggt cggatgggca attaccggaa ttgttgcaaa acttgttatg
     2761 gaaccaaaag gctttggtat gcagtttgac agggatactt tttcattaac actgcttact
     2821 ataatagaat ttttctttta taaattctat tatcaggaag atcctattgc agccgataag
     2881 gaaaaacaat aaccgcagtt tggtcgc
//
LOCUS       DASWBY010000079         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_78407, whole genome shotgun sequence.
ACCESSION   DASWBY010000079 DASWBY010000000
VERSION     DASWBY010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78407"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VF870_01690"
     CDS             complement(<1..526)
                     /locus_tag="VF870_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEX9250917.1"
                     /translation="MVRSEKKHRIIFIDLMRAFAVFQMVQGHTIDALLAPEFRSEQFP
                     VYFLWNFMRGMTAPIFMFSAGTVFTYLFRLVDEPFVINPRVKKGFFRFLLLVSIGYLL
                     RYPTYTIFDFSNVTKDRLDIFFAVDVLQLIGFGILFVMVCAYIAEKLKLSDTIVFTFF
                     ALCFFLATPFSVKIN"
     gene            complement(649..>1824)
                     /locus_tag="VF870_01695"
     CDS             complement(649..>1824)
                     /locus_tag="VF870_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX9250918.1"
                     /translation="LRKDAMALQRLKEAAEKAKIELSTSQSTDVNLPFITATQDGPKH
                     LNLNLSRAKFEQLIDDLVKNTKGPCEQAMKDAGVTPKDINEVILVGGSTRIPMVQQLV
                     KDLFQREPHKGVNPDEVVAIGAAIQGGVLAGDVKDVLLLDVTPLSLGIETLGGVMTRL
                     IESNTTIPTKKSEVFSTAADNQPSVEIHILQGERPMAADNRTLGRFHLDGIPPAPRGM
                     PQIEVTFDIDANGILHVAAKDKATSKEQSIRITSSSGLDKSEIEKMKNAAKEHAAEDK
                     QKKESVEVRNQADSLVFQTKKQMEELKEKVPADVKSRLEAEMKKVEDALAANNTQEIK
                     SATESLSKVWNEVASQLYSQAGPQGQPAPDQQQENKAADDKGDVQDASYEVVDDDKK"
BASE COUNT          570 a          359 c          294 g          601 t
ORIGIN      
        1 aattaatttt tacagaaaac ggagtggcaa gaaaaaaaca tagagcaaaa aatgtaaata
       61 caattgtatc gctaagtttc aatttttctg cgatgtaagc acataccatt acaaacaata
      121 ttccgaatcc tattaactgt aaaacatcta cagcaaaaaa gatatctaat ctatccttag
      181 ttacgtttga gaaatcaaaa attgtgtacg ttggatatct aagtaagtat ccgattgaaa
      241 caagcagtaa aaatctaaaa aaaccttttt taactctcgg attaatcacg aatggttcat
      301 cgacaagccg aaaaagatat gtaaaaactg tacccgctga aaacataaat ataggagcag
      361 tcatgccacg cataaaattc cataagaaat aaacaggaaa ttgttctgaa cgaaattctg
      421 gtgccagtaa tgcatcaatt gtatgacctt gcaccatttg aaatacagca aatgcacgca
      481 taaggtcaat gaatattatt cggtgtttct tttcgcttcg aaccatgagt gatggaatat
      541 tatctttagg tctaaaataa ccaaatgaac tgtaaacaaa aaagctccgg ttactaatta
      601 accggagctt taattgatct taaataaaca aaaatatttt aagctaaatt attttttatc
      661 atcatcaaca acttcataag aagcatcctg aacatcaccc ttgtcatctg cagctttatt
      721 ttcctgctgc tgatctggtg caggctgacc ctgaggacca gcttgagaat acaattgaga
      781 tgcaacttca ttccatactt tacttaaaga ttcagtagca gatttaatct cttgagtatt
      841 attggcagct aaagcatcct caactttttt catttctgct tctaatctgg attttacatc
      901 agcaggtact ttttctttta attcttccat ctgtttcttt gtctggaata ccaaactatc
      961 cgcctgattt ctgacttcaa cagattcttt tttctgttta tcttctgcag cgtgttcttt
     1021 agctgcattt ttcatctttt caatttcact cttatccaat ccgcttgaag aagtaattct
     1081 aatactttgt tccttactcg ttgccttatc ttttgctgca acgtgtaaaa tgccattcgc
     1141 atcaatatca aaagtaactt ctatctgagg catacctcgt ggagcaggtg gaataccatc
     1201 aagatgaaat cttcctaatg tcctgttatc tgcagccatt ggtctttcac cctggaggat
     1261 gtgaatctca actgaaggtt gattatcagc agcagtagaa aatacttcac tcttctttgt
     1321 aggaattgtt gtgttagatt caattaatcg ggtcataaca ccaccgagcg tttcaattcc
     1381 aagggaaagc ggagtaacat ccagtaaaag gacatcctta acatcaccgg ccagaacacc
     1441 gccttgtatt gcagcaccaa tagctactac ttcatcagga ttaacgcctt tatgcggttc
     1501 tctttgaaat aaatctttta ctaactgctg taccattggt attcttgtag atccgccaac
     1561 aagaataacc tcattaatat ctttgggtgt aacaccggca tctttcatag cttgttcgca
     1621 aggaccttta gtatttttga ctaagtcatc gataagttgc tcaaattttg cacgactaag
     1681 atttaagtta agatgtttag gaccatcttg tgtagctgta ataaatggca gattgacatc
     1741 tgttgattgt gaagttgata gttcaatctt tgctttttca gcagcttctt ttaatctctg
     1801 aagtgccatt gcatcttttc taag
//
LOCUS       DASWBY010000080         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_40797, whole genome shotgun sequence.
ACCESSION   DASWBY010000080 DASWBY010000000
VERSION     DASWBY010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40797"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF870_01700"
     CDS             complement(<1..231)
                     /locus_tag="VF870_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX9250919.1"
                     /translation="MVDLSFKYKVLLDFLKDESPFKPDLSIILGSGLGDFAEQLQIHK
                     SISTLDIPGYPESTVVGHKGKIHFAEYENKKII"
     gene            complement(231..>1511)
                     /locus_tag="VF870_01705"
     CDS             complement(231..>1511)
                     /locus_tag="VF870_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX9250920.1"
                     /translation="KRKINWRLVISGFGLQIIFAILILRGDSLRNYFSPLGCPKDFFN
                     SVSYIFVLILNFTTEGAKFVFGSLALAPGNEGNLGFFFAFQVLPTIIFFASLMSVFYY
                     LGIMQKIVQGMAWVMAKVMGTSGAESLSCTANIFVGQTEAPLMIKPFIKGMTQSEMLT
                     IMIGGMATIAGGVMAAYIQMLGSSYAASHGIPLIEAQQMFATQLLGASVMAAPGAMMI
                     GKILIPETSESETKGTIKVSIEKNSSNVIEAAANGAGDGLQLALNVGAMLIAFIAFIA
                     LINYLLSGFGDLVGINSYLISEFGQPLSFQLMIGLVLQFVAFGIGVPWQDSLTFGSLL
                     GTKIVVNEFVAYSDMGNLVQAGKLVNEKSILMATYALCGFANFSSIAIQIGGLGPLAP
                     NRRSDIAALGIKAVIGGVLTTLLTATLAGLFFSF"
BASE COUNT          512 a          306 c          250 g          443 t
ORIGIN      
        1 aattattttt ttgttttcat attcggcaaa atgaatttta cctttatgtc caacaactgt
       61 tgattcggga taacctggga tatctaaagt tgaaatagat ttatgtattt gcaattgctc
      121 agcaaaatct ccaagtccgc tgccaagaat tattgataga tctggtttga atggagattc
      181 gtcttttaag aaatctaaaa gtactttgta tttaaaagat aaatctacca tcagaatgaa
      241 aagaataatc cggctaaagt tgcagttaat aaagttgtaa gtacaccgcc gataacagct
      301 ttaattccaa gagcggcaat atctgatctt ctatttggtg caagtggtcc aagtccgcct
      361 atctgaattg cgatggaaga aaagtttgcg aatccgcata aggcataagt tgccataaga
      421 attgattttt cgttcacaag tttaccagct tgaacaagat ttcccatatc tgagtatgca
      481 acaaattcat ttaccacaat tttggtccca agtaaacttc caaatgttaa actatcctgc
      541 catggaacac cgattccaaa tgcaacaaac tgcaaaacca gaccaatcat aagttgaaaa
      601 cttaatggct gtccaaattc tgaaatcaaa taagaattaa ttccaactaa atctccaaaa
      661 ccacttaaca gataatttat caaagcaatg aaagctataa atgcaattaa catggcgcca
      721 acatttaatg ccaactgcaa accatcacct gcgccattgg cggcagcttc aattacatta
      781 cttgagttct tttcaattga aactttaata gttccttttg tttcggattc agaagtttcc
      841 ggaattaaaa tttttccgat catcatagca cccggagcag ccattacgct ggctcccaga
      901 agttgtgtag caaacatttg ctgtgcttca ataagaggaa ttccatgtga tgctgcataa
      961 gaacttccca gcatctgtat gtaagcggcc ataactcctc ctgcaattgt agccattccg
     1021 cctatcataa ttgtcagcat ctcactttgt gtcattcctt taataaaagg ttttatcatt
     1081 aaaggagctt cagtttgccc gacaaaaata tttgctgtgc aggataagga ttccgcacca
     1141 cttgttccca taacttttgc cataacccag gccataccct gaacaatttt ctgcatgata
     1201 cccaggtaat aaaatacaga catcaaactt gcgaagaaga ttattgtagg aagaacctga
     1261 aatgcaaaga agaacccgag attgccttca ttaccggggg cgagtgcaag actaccaaaa
     1321 acaaattttg cgccttcagt cgtaaagttt aatatcaaaa caaaaatata actaactgaa
     1381 ttaaaaaaat ctttaggaca tccaagcggg gaaaaatagt ttcgtagtga atcaccacga
     1441 aggataagaa ttgcaaaaat tatttgaagc ccgaatcctg atataactaa tcgccaattt
     1501 atttttctct t
//
LOCUS       DASWBY010000081         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_303963, whole genome shotgun sequence.
ACCESSION   DASWBY010000081 DASWBY010000000
VERSION     DASWBY010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303963"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VF870_01710"
     CDS             <1..566
                     /locus_tag="VF870_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9250921.1"
                     /translation="NRVLEVKSIDFQKISDTKSPQGIAAVFNKNYPKKNLNELKDKLV
                     VLIDNISDPGNLGTIIRTCDWFGVYNILISDQSVEYLNPKVIRATMGSLFHLNIYDEI
                     YENDLANLKSNGYQIVCADTKGKNIFSYKTNKKTVVAFSNESLGPSEMIKSLSDDFIT
                     IPGKGRAESLNVSSAAAIILSRLAIDI"
     gene            complement(580..1794)
                     /locus_tag="VF870_01715"
     CDS             complement(580..1794)
                     /locus_tag="VF870_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX9250922.1"
                     /translation="MKRIKIFGREIIDEKSIAQIERCTSENDIGVLTADAHYGYGHPI
                     GGAVAYKNKISLSGVGFDIACGNKAARTDIKADEIQLPKIMDEIYNQISFGIGRKNNQ
                     PVDHEVLDKISSAEFKPQRKLISLAKEQLGTVGSGNHFVDLFKDDDGFLWIGVHFGSR
                     GFGHRTTMGFIALSQNLSFEDKAKEGSMDAPPILFDIHSEIGQAYLEAMHLAGEYAYA
                     GRDKVVELVLRILGNPQKTFEVHNHHNFAWREEHFGEKYWVVRKGCTPAFPNQLGFIG
                     ANMMDDSVIVEGIDSQLSKEALYSTVHGAGRTMSRNEAAGKKKWIRDKDGIKKPVTVS
                     RGKVNFEKVKSTVRQKGIELRGSGADEAPECYKNLDKVLEFMGNTIKVLYRLHPIGVA
                     MAGSDVYDPYKD"
     gene            complement(1935..>2245)
                     /locus_tag="VF870_01720"
     CDS             complement(1935..>2245)
                     /locus_tag="VF870_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX9250923.1"
                     /translation="EQNYPNPFNPSTVIRYAVPNITQGGIEESRVILKIYDILGREIK
                     TLVNQEQTSGVYEVIWNGDDELGNKVSTGVYFYRIDAGYPSSGSGQSFVQTKKMMLIK
                     "
BASE COUNT          668 a          449 c          368 g          760 t
ORIGIN      
        1 caaacagagt actggaagta aagtcaattg attttcaaaa aatatcagac acaaaatctc
       61 cccagggaat agcggcagta ttcaacaaga attatcctaa aaaaaatctt aacgaactaa
      121 aagataaact ggttgttctt attgataata tatcggatcc cggtaacctt ggtacaataa
      181 taaggacctg cgattggttt ggtgtttaca atattctaat ttcggatcaa tcggttgagt
      241 atcttaatcc aaaagtaatt cgcgctacaa tgggatcgct ttttcatctt aacatttatg
      301 atgaaatata tgaaaatgat ctggctaatc ttaagagcaa tggttaccag attgtttgtg
      361 ctgatacgaa aggcaaaaat attttttcat acaaaaccaa caaaaaaact gttgttgctt
      421 tttcaaatga atctttaggg ccttctgaaa tgattaaaag tctttcagat gatttcataa
      481 caattcctgg taaaggcaga gcggaatctt taaatgtttc atcggctgct gcaataattt
      541 tatccagact agcaattgat atataaaaaa taaaatcttt taatctttgt aaggatcata
      601 aacatctgaa cctgccattg caacacctat tggatgaagt ctgtaaagta ctttgatagt
      661 atttcccata aattctaaaa ctttatcaag gtttttgtag cattccggag cttcatctgc
      721 tcctgaacca cgcagctcaa ttcccttttg ccgaacagtc gattttactt tttcaaaatt
      781 aactttacct ctgctaactg ttaccggctt tttaattcca tctttgtctc ttatccattt
      841 tttctttccg gcagcttcgt tgcgggacat tgttcttccc gcaccatgta cagttgagta
      901 caatgcttct ttagaaagtt gtgaatcaat accttcaaca attacggaat cgtccatcat
      961 attggcaccg ataaagccaa gctgatttgg aaaagcaggt gtacatcctt tgcgcactac
     1021 ccaatatttt tcaccaaaat gttcttcccg ccaggcaaag ttatgatgat tatgtacttc
     1081 aaatgttttt tggggattac cgagaattct taagaccagt tctacgactt tatctcttcc
     1141 agcataagca tattcgcctg caagatgcat cgcttctaaa taagcttgtc ctatttcgga
     1201 atgaatatca aacaaaatcg gcggcgcatc catcgatcct tctttggctt tatcttcaaa
     1261 tgataaattt tgtgaaaggg caataaatcc cattgttgtt ctgtgcccga atccacgcga
     1321 accgaaatga acaccaatcc acaaaaatcc atcatcatct ttgaacagat ccacaaagtg
     1381 attaccgctt ccaaccgttc ctaattgttc tttggcgagt gaaataagtt tacgctgagg
     1441 tttaaattct gctgaagata ttttatctaa tacttcgtga tcaactggct ggttattttt
     1501 tctgccgatg ccgaatgata tttgattata aatctcatcc ataattttag gcagttgtat
     1561 ttcatctgct ttaatgtctg ttcttgcagc tttgttgccg cacgcaatat caaatccaac
     1621 accggataga gaaattttat ttttatatgc aaccgcccca ccgattggat gaccatatcc
     1681 gtaatgagca tcagcagtaa gcacaccaat gtcattttca gatgtgcatc tctcaatctg
     1741 tgcgattgat ttctcatcaa ttatttctct gccgaagatt tttatacgtt tcataatatt
     1801 cgatacaaat gacttaatta attataagtt ttttatttaa ctgtgtcatt atataaaaaa
     1861 aggcacgatt gctcgtgcct ttaattattt tgatgaagtc acacttcgac tgcgctcagt
     1921 gtgacgatta ttaattattt aatcagcatc atcttttttg tttgcacaaa actctgtcct
     1981 gagcctgacg aaggataacc ggcatcgata cggtagaaat aaactcctgt gctaacttta
     2041 ttgccgagtt catcatcgcc attccaaatg acttcataga ctccgcttgt ttgttcctga
     2101 ttaacgagtg tttttatttc cctgcctaaa atatcatata ttttaagaat cactcgggac
     2161 tcttcgattc ctccctgggt gatattcggt actgcatatc ttataacagt acttggatta
     2221 aatggattgg ggtagttttg ttcaa
//
LOCUS       DASWBY010000082         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_228866, whole genome shotgun sequence.
ACCESSION   DASWBY010000082 DASWBY010000000
VERSION     DASWBY010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228866"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VF870_01725"
     CDS             <1..1563
                     /locus_tag="VF870_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX9250924.1"
                     /translation="GAIGAAVLNSLRETDIRFGQFISVGNKADVNENDLLEYWQDCKD
                     VSVITYYLESFVDGEKFIKYFIDEKISKPVIVLKGGRTSSGIKAASSHTGALGSSDKV
                     VDAILKQFGIIRADDLNDMFNTAKGFEDFPAPKGNRVAVVTNAGGPAILTVDTLEKNN
                     LTLADLSLETKLNLKDFVHPEGSVNNPVDLLPGGTAEQFKAVNEILVKDKNVDAVISV
                     FVEPIMVKALPVIESINDIKSEKPIFQVVMPLPEFWQEYRTESKTKRALFKRPEDPAV
                     VINNLLKFRNKAGNAGRIKPKQSTKDFKLNEKPGRFLSTNEVAELCNYYKLPLIKDEV
                     LSFDQLMKKHINFPVVLKAVGEKIVHKTDLKGVFLNIETKDDLIKTAEEMIWNFSAKN
                     IQLDSFLVQPFLKTKFELLVGAFRDPSFGPMIMFGSGGKYVEYLDDTVMRSAYLTDND
                     IEGMINSTKIGKIIQGVRGEESADMTSIKFSVKNLAQMMVNHKEITEVDLNPLLITSD
                     NKIFAVDVRIKC"
     gene            1652..>1908
                     /locus_tag="VF870_01730"
     CDS             1652..>1908
                     /locus_tag="VF870_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250925.1"
                     /translation="MNRKKFITSLSVASFGATVFPYETLAKPSGSGSRNPESLFKDEP
                     PVIKPPRLKAGDKLAIVAPGSYISQEELQDSIKNLNALGFET"
BASE COUNT          667 a          284 c          386 g          571 t
ORIGIN      
        1 ggagcgattg gtgcggcagt attgaattca ttgcgcgaaa ctgatatacg ttttgggcaa
       61 tttataagcg ttggaaacaa agctgatgta aatgaaaatg atttattaga atactggcag
      121 gattgtaaag atgtaagtgt aataacttat tatttagaaa gttttgttga tggtgaaaag
      181 ttcatcaaat attttattga cgaaaagata tccaaaccgg ttatagtgct taaaggcggc
      241 agaacatcaa gcggaatcaa ggccgcttca tcgcatacag gcgcgctggg cagcagtgat
      301 aaagttgtgg atgcaatttt aaaacagttt ggaattattc gtgctgatga tttaaatgat
      361 atgtttaata ccgcaaaagg atttgaggac tttccggcac caaaaggaaa tcgtgttgct
      421 gttgttacaa acgcaggcgg acccgccatt ttaactgttg atacacttga gaaaaacaat
      481 ctcactcttg cagatttaag tctggaaaca aaattaaacc ttaaggattt tgtacatccg
      541 gaaggaagcg tcaataatcc tgttgattta cttccgggcg gaacagcgga gcagtttaaa
      601 gctgtaaatg aaattcttgt taaagataaa aatgttgatg cggtaatttc agtttttgta
      661 gaaccgatta tggttaaagc cctgccagtt atagaatcca taaatgatat taaatccgaa
      721 aaaccaattt ttcaggttgt tatgccgctt cctgaatttt ggcaggagta cagaactgaa
      781 tcgaaaacaa aacgagcctt gtttaaaaga cctgaagatc cggcagtggt aattaataat
      841 ttgcttaagt tccgaaacaa agcaggtaat gcagggagaa ttaaacctaa acaatcaaca
      901 aaggatttta agctgaatga aaagccggga agatttttat ctacaaatga agtagcagaa
      961 ttatgcaatt attacaagct gccattaatt aaggatgaag ttttaagttt tgatcaattg
     1021 atgaaaaagc atatcaactt tccggtagtg ctcaaggcag tcggagaaaa gatagttcat
     1081 aaaactgatt taaaaggtgt attcttaaat attgaaacaa aagatgattt gataaaaaca
     1141 gcagaagaaa tgatctggaa ctttagtgct aaaaatattc agttggattc atttttagtt
     1201 cagccatttc taaaaacaaa atttgaatta ttagttggtg cttttcgtga tccaagcttt
     1261 ggtccgatga taatgtttgg ttcaggcggc aaatatgttg aatacttaga tgatactgtt
     1321 atgcgatcag cctatctaac tgataatgat attgaaggaa tgattaactc cacaaagatt
     1381 ggaaagataa ttcaaggggt gcggggtgaa gaatcagcag atatgacttc aataaaattc
     1441 tcagtaaaaa atcttgcaca aatgatggtt aatcataaag aaattacaga agttgatttg
     1501 aatccattac tcattacatc agacaataaa atctttgctg tggatgtaag gattaagtgc
     1561 taattaattt gttaacaaac ccagttcctt taagaatttg ggttcgtaag tttcctctcg
     1621 cattttctaa ttatcattcc taaattaaac tatgaacaga aaaaaattta taacatcgtt
     1681 atctgttgca tcatttggag caacagtttt tccttatgaa actcttgcaa aaccttcggg
     1741 atcgggaagc aggaacccgg aaagtttatt taaagatgaa ccaccagtaa tcaaaccgcc
     1801 aagattaaaa gctggtgata agcttgctat tgttgccccg ggaagttata tctctcaaga
     1861 ggaacttcag gattcaatta aaaatcttaa tgcattagga tttgaaac
//
LOCUS       DASWBY010000083         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_604788, whole genome shotgun sequence.
ACCESSION   DASWBY010000083 DASWBY010000000
VERSION     DASWBY010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604788"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VF870_01735"
     CDS             complement(<1..680)
                     /locus_tag="VF870_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250926.1"
                     /translation="MKKNKLVIPFFVVVLLLQVVTLAQYAPRQDAVWARTVPAGTITL
                     DGILNEAAWSQAESFEINYGQDGPLPTSGWRAEFQPDTVTDPTHATVKFLVSSDNQLW
                     LAFDIPDSSVGGNKDWARWDGILASIRSLGADNMAYAAEFFYTYWTAGRQFPDSVKVD
                     GGPYFVSGNTNNAFATWGVDDRTPEQIANWDAKTVIVGGHSNDAGRDEGWVVEMRFNL
                     AALGYDVT"
     gene            complement(740..3115)
                     /locus_tag="VF870_01740"
     CDS             complement(740..3115)
                     /locus_tag="VF870_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HEX9250927.1"
                     /translation="MEQFSTKYGHFSSDGNEYIITNFRTPKPWINLISNGKYGLVISQ
                     TGGGFSWDTHSEFNRLNRWHQDLVQDNWGKYFYVKDNQTGEVWSPTWMPVKTELESYQ
                     IAYGFGYARFISEYKGVKIVLTVFIPVGENLEVWNFNIENKSTQKKSLSIYSYLEWCL
                     GSSSDHHREFHKTFLETHFDSKLNSMTATKRLWEIPLGDRGHWNIEYPYLGFISCSRK
                     ITDYDGDKDTFVGQYGSLQNPIGLSAKSLSKKLGKWNDSIASVKTQMEIESSETKNVN
                     FFIGIKENRNAIASSLKKFNTQKSIDSALFDVKLFWHKMFDTLVIETPDKAMNLLVNK
                     WLRYQAIAGRLLGRTAYYQQSGAFGFRDQLQDSMVFLPIDPKRTEDQIRLHARHQFED
                     GTVLHWWHPISETGLPTKMTDDLLWLPFVLMNYIDETGNYKILSTKEPYYDNKNKKDT
                     LFDHSVAAIEKVLTRLSKRGLTLIGAGDWNDGLSAVGLDMKGESIWLTEFFYLILRRF
                     AELSKEIGNDKLYKRYVKKANELKKAFDKYAWDGDWFWRATKDDGNKVGSKTSPEGKI
                     FLNAQTWSVISGIAESNKQKKAMDSVSKYLLKKNGCLLLSPAYTKPDKMIGYLSRYAP
                     GRRENGGVYTHAATWAVWAYALLKDNDQAFKSYKNLCPVYNGMKPDEYVAETYVTPGN
                     IDGPDSPNYGMGGWTWYTGSAAWYQKVIVDWILGIRASKDGLIIDPCIPDDWKEFSVK
                     RLFRGVTYKIDVINKSKTNKIKKHITVNGKLINSNVIPIIKKGEVKVEVYL"
     gene            complement(3137..>3634)
                     /locus_tag="VF870_01745"
     CDS             complement(3137..>3634)
                     /locus_tag="VF870_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9250928.1"
                     /translation="ATEYLIKKKKFKKIAHITGPAENYDAYLRKKGFIDACKKNGVSI
                     NKSFIIDGNFTIDSGYHGFIQLYGLPEKPEAIFAANDMMALGCYDAANYFNVKIPDDI
                     GLIGFDDIFMSKYISPSLTTVRVQIEEVGKAAARLLIERLNSTNGKTHSLIKIPTELI
                     IRNSC"
BASE COUNT         1100 a          730 c          557 g         1247 t
ORIGIN      
        1 tgggtgacat cataccccag agcagctaaa ttaaatcgca tctcaacaac ccatccttca
       61 tctctgcccg catcgtttga atgtcctcca acgataactg tttttgcatc ccaattagct
      121 atttgttcgg gcgttctatc atcaacgccc caagttgcaa atgcattatt agtattgccg
      181 cttacaaaat atggaccacc atctactttt acactatctg ggaactgcct accggcagtc
      241 caataagtat agaaaaattc agcagcataa gccatattat cagcgcctaa tgaacggata
      301 gatgctaaaa ttccatccca tcttgcccaa tccttattac ctcccactga tgaatcggga
      361 atatcaaagg ctaaccaaag ttgattatca cttgaaacta gaaatttaac agttgcatga
      421 gtagggtcag taactgtatc tggttggaat tcagcacgcc atccactagt aggcagtgga
      481 ccatcctgcc cataattaat ttcaaatgat tctgcttgcg accaggcagc ttcatttaga
      541 ataccatcca gggtaatggt tcctgcggga actgttcttg cccagacagc atcctgtctt
      601 ggggcatatt gtgcaagtgt tacaacttgc agtagcaata ccactacaaa aaatggaatt
      661 accagtttgt tttttttcat agcctctcct aaaggtttgt tttattaatt atgtgtatta
      721 gtgaaaattt tgactgattc tataaataaa cttctacttt tacttcgcct tttttaatta
      781 tcggtattac attactgtta attaatttac cattcactgt aatatgtttt ttaattttat
      841 tagtctttga tttattaata acgtctattt tatatgttac tcctctaaac aatcttttaa
      901 ctgaaaattc tttccagtca tctggaatac agggatcaat tattaatcca tctttgctag
      961 ctctaattcc aagtatccaa tcaacaataa ctttttgata ccaggctgct gaacctgtgt
     1021 accacgtcca accgcccatt ccataatttg gggaatccgg tccatcaatg tttcccggag
     1081 tcacgtaagt ttcagcaaca tattcatcgg gcttcattcc attatatacc gggcataaat
     1141 ttttatatga tttgaatgcc tgatcattat ccttaagcaa tgcataagcc caaacagccc
     1201 acgttgcagc gtgagtatat acgccgccat tttctcttct gccgggagca taacgtgata
     1261 agtaaccgat cattttatcg ggtttagtat aagcaggact taaaagcaag catccatttt
     1321 tcttaagtag atattttgaa acagaatcca ttgctttctt ttgcttattt gattctgcta
     1381 ttccacttat tacagaccag gtttgtgcat taagaaatat tttaccctca ggtgaagttt
     1441 tactaccgac tttgttacca tcatcttttg tagctcgcca gaaccaatct ccatcccagg
     1501 catatttatc gaaagctttt ttaagctcat tagctttttt aacgtacctc ttataaagtt
     1561 tatcattacc aatttctttt gaaagttcag caaatcgtct caagatcaaa tagaaaaatt
     1621 ccgttagcca gatagattct cctttcatat ctaaaccaac agcactaagt ccatcattcc
     1681 aatcaccggc accaattaaa gttaaaccgc gcttgcttaa tcttgtcaac actttttcaa
     1741 ttgctgctac agagtgatca aatagagtat cttttttatt tttattatca tagtaaggtt
     1801 ctttggttga taggatttta taattaccag tttcatctat gtaattcatc aatacaaatg
     1861 gaagccaaag aaggtcatct gtcatttttg taggcaatcc tgtttcagaa ataggatgcc
     1921 accaatgcag aacagtgcca tcctcaaatt gatgcctggc gtgcaagcga atttggtctt
     1981 ctgttctctt aggatcaatt ggaagaaaaa ccatactatc ctgcaattga tctctgaatc
     2041 caaaagcacc gctttgctga tagtatgcag ttcgtcccaa aagtcttccg gcaattgcct
     2101 gatatcttaa ccacttgtta accaggagat tcatggcttt gtcaggagtt tcaataacaa
     2161 gtgtatcaaa cattttgtgc caaaataatt ttacatcaaa tagagcacta tcaatacttt
     2221 tttgagtgtt aaatttttta agagatgaag cgattgcatt tctattttct ttaattccaa
     2281 taaaaaagtt tacgtttttt gtttcacttg attcgatttc catttgggtt ttaactgaag
     2341 cgattgaatc attccatttg cccaattttt tacttaaact ttttgcagat agtcctatgg
     2401 gattttgtaa agacccatat tgcccaacaa aagtatcttt gtcaccatca taatctgtaa
     2461 tttttcttga gcatgaaata aatccgagat aaggatattc gatattccag tgccctctat
     2521 caccaagtgg aatttcccac aatcgttttg tggcagtcat actgtttaat ttagaatcga
     2581 aatgagtttc taaaaacgtt ttatgaaact ctctatgatg atcagatgaa gaaccaaggc
     2641 accattcaag gtaagagtaa attgaaagag attttttttg agttgattta ttttcaatat
     2701 taaaattcca cacttcaagg ttttcaccaa cgggaatgaa aaccgttaat acaattttaa
     2761 cacctttata ttctgagatg aatcttgcat atccaaaacc ataagcaatc tgatatgact
     2821 caagttcagt tttaacaggc atccaggtag gagaccatac ctcacctgtt tgattatctt
     2881 ttacataaaa atattttccc caattatcct gcactaaatc ctgatgccag cggtttagtc
     2941 tattaaactc ggaatgagta tcccaactaa atccaccgcc agtctgtgaa ataactaatc
     3001 catatttacc attggatatt aaatttatcc agggcttggg agtcctgaaa tttgtaataa
     3061 tatattcatt cccatcagaa gaaaagtgtc catactttgt agaaaattgt tccatcaagt
     3121 ttccatttat aaaatattaa caggaatttc taataattaa ttcagttgga attttaatta
     3181 gagaatgggt cttgccgttt gtactattta gtctttcaat taaaagtctt gcagcagcct
     3241 ttcctacttc ttcaatttga actcttacag tagtaagact gggacttata tattttgaca
     3301 tgaagatatc atcaaatcca attaaaccta tatcatcagg aattttgaca ttgaaatagt
     3361 ttgcagcgtc atagcagcca agcgccatca tatcattagc tgcaaaaatt gcttcgggtt
     3421 tttctggtaa accatacagt tgtataaatc catggtaacc ggaatcaatc gtaaaatttc
     3481 catcaataat aaaagattta ttgatgctta caccattttt tttgcaggca tcaataaatc
     3541 ctttttttct aagatatgca tcatagtttt cagccggacc ggttatgtga gcaatttttt
     3601 taaatttttt ctttttaatc aaatattctg ttgc
//
LOCUS       DASWBY010000084         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_116116, whole genome shotgun sequence.
ACCESSION   DASWBY010000084 DASWBY010000000
VERSION     DASWBY010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116116"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VF870_01750"
     CDS             <1..694
                     /locus_tag="VF870_01750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9250929.1"
                     /translation="GIDVQGKQIIDIGCGTGIISLLALKMGAKKAVCGDISEYMLKLA
                     QENYTTAGYDSTRVNLCQLDAESLPFDANSFDAAITGMSFGLFPDQKKAVNEMFRVLR
                     PGGLISLGAHGPEHYWEAIDTTIRAVNKRNVLGYRFEFWPRTEKQIRNIMKNSGFINV
                     QTYRYIWRNLFKTPADACDFFAAVTSNWWYAKIPESKRLHEYQKAKHLFEAREIRQVT
                     DDVIIGYGEKPE"
     gene            complement(801..1301)
                     /locus_tag="VF870_01755"
     CDS             complement(801..1301)
                     /locus_tag="VF870_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9250930.1"
                     /translation="MKKNYFLLFVVAIIIVSCVPKTKTVPVDTAAVKAEVTTVLDKWH
                     AAYQGKDLPVLMSLFSDDLLFCGPAPNEFWDKSTLSPLFERPFADTSFVISYSIIERN
                     IKVVKDGTSAISTEHFFLDKASKKIQLRQVCHLVKSEGIWLIDYVSVAYIPNREDMGK
                     INKALE"
BASE COUNT          498 a          270 c          306 g          467 t
ORIGIN      
        1 tggaatagac gtgcagggca agcaaattat cgacataggt tgcggtacag gtattatttc
       61 acttcttgca cttaaaatgg gtgccaaaaa agcagtatgt ggagatatct cggaatatat
      121 gctaaaacta gcccaagaaa attatacaac ggctggttat gattcaaccc gcgtcaatct
      181 ctgccagtta gatgctgaat ccttaccttt cgatgctaat agttttgatg cagcaataac
      241 agggatgtca tttggattat tccctgatca gaaaaaagca gtcaatgaaa tgtttcgcgt
      301 tctgagacca ggaggtttaa tttccttagg agctcatggc ccagaacatt attgggaggc
      361 tattgatact actattcggg ctgttaataa aaggaatgtt ctgggttatc gtttcgagtt
      421 ctggccaaga accgaaaaac agatccgtaa cataatgaaa aattctggat tcataaatgt
      481 ccagacatat agatacatct ggcgaaacct gttcaagaca ccagctgatg cctgtgattt
      541 ttttgcggct gttacttcaa actggtggta cgcaaaaatt cctgaaagta agagattgca
      601 tgaatatcag aaagcaaaac atttatttga agccagggaa atcaggcagg ttacagatga
      661 tgtgattatt ggctacgggg aaaaaccaga ataagaaact cccaacagga aattattaaa
      721 ttacatattt cttttcagac cttcttttga agtggtcaaa gaaggatgga ttttgaagaa
      781 aattaatttg atattgaaaa ttactccaat gccttattga ttttgcccat gtcttccctg
      841 tttggaatat aagcaacgct aacataatca attaaccaaa ttccttctga cttaacaagg
      901 tgacatacct ggcgtaattg aattttcttg ctagccttat cgagaaaaaa atgttcagta
      961 gaaatagctg aagtaccgtc tttcacaacc tttatgttac gctcaattat tgaataactt
     1021 atcacaaagg aggtgtcagc aaaagggcgt tcaaataaag gggataaagt cgacttgtcc
     1081 cagaattcat ttggtgcagg gccgcaaaag agtaagtcat cagaaaacaa agacattaaa
     1141 acggggagat cctttccctg gtatgcagca tgccatttgt ctaacacggt ggttacttca
     1201 gcttttactg ctgctgtgtc tacaggaact gttttagttt tgggtacgca cgatacgatt
     1261 attatcgcta ccacaaacaa taagaaataa ttctttttca taaattattg gtattaaagt
     1321 ggttcattgc atacaattta aggtatttaa ccgaaacaaa cattatttac taaagtttgc
     1381 cataaaccca gagagctttt catcagctca atttgattga gctttgtctc agtggtttgc
     1441 gcttgtatcc cattcataca gattataaag aataaaacag ctgcggtagt tgaatatgct
     1501 ttcttcattt cattggattt tagtggatgt taaaagctta a
//
LOCUS       DASWBY010000085         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_3611, whole genome shotgun sequence.
ACCESSION   DASWBY010000085 DASWBY010000000
VERSION     DASWBY010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3611"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VF870_01760"
     CDS             complement(<1..691)
                     /locus_tag="VF870_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9250931.1"
                     /translation="MSTLMVSISGIRGIIGDGLDPEVIVKYTNAYADFVGTGKVVVGR
                     DARITGEMVNQIVTGTLLAKGLDVIDIGICPTPTVQYTVKSLNAQGGIAISASHNPNQ
                     WNALKLLNSTGQFMSPDEHQQMKKYLDIQNNFVDWTKIGKRTVYSEGLTNHINAVLAL
                     PIIDVNKIKSKKFKVLADCVNGAGAYCIPDLLRTFGCEVIEMNCEKTGIFPRLPEPLP
                     ENLIETMNAVKK"
     gene            757..1464
                     /gene="nth"
                     /locus_tag="VF870_01765"
     CDS             757..1464
                     /gene="nth"
                     /locus_tag="VF870_01765"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9250932.1"
                     /translation="MKNKIQKINSLLIEHFGIPPRRKKLPSPLDAIIGTILSQNTNDK
                     NSYQAYRNLKDNFKNWDELTLLKASEIEKYIRVAGLGRQKSNAIYQLLQSLKRRQKSV
                     SLNYIKTKPDVEILEELTSYNGVGVKTASCVLLFSLDRNICPVDTHVHRTLNRIGLVK
                     TNSPEKTFYEILNKIPDGTAHSFHTNLIRLGREICKSTNPNCSYCPLLKICKYEKKNL
                     NPHKTQKTIDFMLLDNL"
     gene            1554..>1884
                     /locus_tag="VF870_01770"
     CDS             1554..>1884
                     /locus_tag="VF870_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9250933.1"
                     /translation="MSNNLGLIGYDFVEFYVGSAKMWAYWHAKAMGLHVKAYLGPETG
                     NRDRVSYYLEKNNVRIVLTSAVKPSTYDVASFVEKHGDGVKRWSVRVNDVSKSFEASL
                     QNGGIPVR"
BASE COUNT          640 a          339 c          301 g          604 t
ORIGIN      
        1 atttttttac agcattcatt gtctcgatta aattctccgg gagtggttca ggcaggcgag
       61 ggaagattcc tgttttttcg caattcattt caattacttc acaaccaaac gttcttagca
      121 aatccggaat acaataggct cctgcaccat taacacaatc agctagtact ttaaactttt
      181 tggatttgat tttattaaca tctatgatag gcaaagctaa aacagcattt atatgattcg
      241 tcaatccttc agaataaaca gtccttttac caatcttcgt ccaatcaaca aaattatttt
      301 gaatatccaa atattttttc atctgctgat gctcatcagg agacataaac tgtccggtac
      361 tgttcaacaa tttcaaagca ttccattgat ttggattatg actcgctgag attgcaatac
      421 cgccctgtgc atttaaagat ttaactgtgt actgcacagt tggagtcggg caaattccaa
      481 tatcaattac atccaatcct tttgcaagca atgttccggt cacaatttga tttaccattt
      541 ctccggttat tcttgcatca cgcccaacca caacttttcc ggttccaaca aaatctgcat
      601 aagcgttcgt gtatttaaca attacttctg gatcgagacc atctccaata attcctcgga
      661 ttccggaaat actaaccata agagttgaca ttaaatctcc aaaaattttc ttctaaaata
      721 atgatttaat tgaatatctt tgtaaaaata ttttgtatga aaaataaaat ccaaaaaata
      781 aacagccttt tgattgaaca ttttggtatt ccgccaagac ggaaaaaact tccatcaccc
      841 ctggatgcga ttatcggaac aatattatct cagaacacaa atgataaaaa ttcatatcag
      901 gcatatagaa atctaaaaga taattttaaa aattgggatg agcttacgct tcttaaagca
      961 tcagaaattg aaaaatatat tagggtagcc ggtcttggga gacaaaaatc aaatgctatt
     1021 taccagctgt tgcaatcact taagagacgc caaaaaagtg tttccttaaa ttatatcaaa
     1081 acaaaacctg atgtagaaat tcttgaagag ttaacttcat ataatggagt aggagtgaaa
     1141 actgcaagct gcgttttact attctcatta gacagaaata tctgtccggt tgatactcac
     1201 gttcatcgta cattaaatag aataggattg gtaaaaacaa attcacccga aaaaacattt
     1261 tatgaaattc taaataaaat tcctgacggt acagcccatt catttcacac aaatttaata
     1321 agattaggca gagagatttg taaatctaca aatccaaatt gttcatattg tcctttactc
     1381 aaaatttgca agtatgaaaa gaagaatcta aatccacata agacacaaaa aacaatagat
     1441 tttatgcttc tggataatct ttaattaaga ttcatattct acttttatct aaaaactatt
     1501 tctcttaact ttgaaataat tattaacccc aataactaat cggagatttt tgaatgtcta
     1561 acaatttagg tttaattggt tatgattttg ttgagtttta tgttggctca gctaagatgt
     1621 gggcttactg gcatgcaaag gctatgggct tgcatgtaaa ggcttatctt ggaccagaga
     1681 cgggtaacag ggacagagtt tcgtattacc ttgaaaaaaa taatgtaaga attgttctta
     1741 cttcggcagt aaaaccatct acttatgatg ttgcttcatt tgttgagaaa catggagatg
     1801 gagtgaaacg ctggtcagtc cgtgttaatg atgtaagcaa atcttttgaa gcatctttgc
     1861 agaacggcgg tattcctgtc cgat
//
LOCUS       DASWBY010000086         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_304055, whole genome shotgun sequence.
ACCESSION   DASWBY010000086 DASWBY010000000
VERSION     DASWBY010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304055"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VF870_01775"
     CDS             complement(<1..321)
                     /locus_tag="VF870_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX9250934.1"
                     /translation="MKEYAVIGLGSFGFNIAKKLASIGNKVVAVDINPEKVDEIKNFV
                     HDAVIGDIKNKTFLQEFIDDKIDGVVISIGSNMVDSILAVHYLKAMNVNNLIVKANNE
                     VHAEL"
     gene            complement(336..1679)
                     /locus_tag="VF870_01780"
     CDS             complement(336..1679)
                     /locus_tag="VF870_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX9250935.1"
                     /translation="MLKSIEKVLSPIQLLSLGYVVIILLGAIILMLPISSTNNSYQFF
                     VDSLFTSASAVSTTGLVVVDTGTYYSTFGEIVILILIQIGGLGYMLFFVVFTVLLTEK
                     LSISGKKYLREAVTRNQSVDLIKFVKLTLIYTLVIEGIGTLGYYLVWVNKYPAVYALY
                     SSFFHSVSAFCTAGFGLYPDSLSSYSKSIIINFNTFFLVITGGIGFFVLYDIINFAKQ
                     KIKKNNIARLSLHSKLVLSFTFSLFVFGTVIILISEPYNLSENILQNILNAFFQSVSA
                     STTVGFNTVDITIMSNASLFVMIILMFIGASPGSTGGGIKTTSFAASVLSSIASLKGK
                     NAVTIFKRTISHVSINKANAQIFVALTSTIIITLILSITEKFSFIKILFEATSAFGTV
                     GLSAGITSGLSTIGKLLITILMIIGRVGPLAIGLFFVNKNGNNNYAYPEEEIFTA"
     gene            complement(1869..>2171)
                     /locus_tag="VF870_01785"
     CDS             complement(1869..>2171)
                     /locus_tag="VF870_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9250936.1"
                     /translation="VVNLLSNAIRYSSKKGTISIEATQENQFIRISVKDDGPGISVQE
                     QNRIFEKYVKSKTEPAKGTGLGLAIAKEFVEVQGGIIGVKSKPGEGSTFYFTLPIA"
BASE COUNT          725 a          363 c          324 g          759 t
ORIGIN      
        1 caactctgca tgaacttcat tatttgcttt tacaattaga ttgttaacat tcattgcttt
       61 cagatagtga acagccagga tactatcgac catatttgaa ccaatgctta ttacaacacc
      121 atctatttta tcatcaataa attcctgtaa aaatgttttg tttttgatat caccaattac
      181 agcatcgtga acaaagtttt ttatttcatc aactttctct ggatttatat ctaccgctac
      241 aactttatta ccaattgaag caagtttttt agcgatgtta aatccaaaac tacctaatcc
      301 tataactgcg tactctttca ttttatctcc tgttattatg ctgtaaatat ttcttcttcc
      361 ggataagcgt aattattatt tccatttttg tttacaaaga ataaacctat tgccaatggt
      421 ccaacccgac cgataatcat caatattgtt attaacaatt ttcctatcgt acttaatcct
      481 gatgttattc ctgcagataa tcccacagtt ccaaatgcgg atgttgcttc aaaaagtatt
      541 tttataaaac taaatttttc cgttattgag agtataagcg taattattat tgtggaggtt
      601 agtgctacaa atatctgagc atttgcttta ttaatactga catgacttat tgttcgctta
      661 aaaattgtaa ctgcattttt accttttagc gatgctattg aggaaagtac gcttgctgca
      721 aacgaagttg tcttaattcc acctccagtg cttccgggtg atgcgccgat aaacattaat
      781 ataatcatta caaataaact ggcattcgac attattgtta tatctacagt gttgaaccca
      841 actgttgtgg atgcagagac agattgaaag aaggcgttta atatattttg taaaatattt
      901 tcagataaat tataaggttc tgaaattaag attattacag taccgaaaac aaaaagtgaa
      961 aaagtaaatg ataaaacaag tttcgaatga agtgacaatc gagcaatatt atttttttta
     1021 attttttgct tggcgaaatt tataatatca tatagaacaa aaaatccgat cccacctgtg
     1081 attacaagaa agaaagtatt aaaattaatt attatgcttt tgctgtaaga gctaaggcta
     1141 tctgggtata atccaaaacc agctgtacaa aacgctgata ctgaatgaaa gaaagatgaa
     1201 taaagtgcgt aaacagcggg atatttgttt acccaaacta aataatatcc taacgtacca
     1261 atgccttcaa taacaagagt atatattaaa gtaagtttaa caaactttat taaatcaaca
     1321 ctttgatttc ttgtgacagc ttcacgcaaa tacttcttcc cgcttatcga tagtttctct
     1381 gttaaaagca cagtaaacac gacgaagaaa agcatatatc ccagtccacc gatttgaatt
     1441 agaattaata ttactatttc tccaaatgtg gagtaataag tcccagtatc aacaacaact
     1501 aaacctgttg tactcacagc agaagctgaa gtgaaaagag aatcaacgaa gaattgatat
     1561 gagttgtttg ttgatgaaat tggcagcata agtataattg ctcccaacaa aataattact
     1621 acatacccaa gacttaataa ctgtatagga ctgagtactt tttcaattga ttttaacatt
     1681 ttagtctaat ttagatttaa ttattacttc taaatgctta ttagacaatt actattccaa
     1741 ttaattgtta gtaattggaa gttgaaatga taagtaatta aggattaaat gcgatcagat
     1801 tctgtttatc tagtgattga gatgcaggca tttttcagag tggaaaaatg attttcaaaa
     1861 tggaaatact atgctattgg gagtgtaaaa taaaatgtgc ttccttctcc gggtttgctt
     1921 ttaacaccga ttataccgcc ttgcacttca acaaattctt tggcgattgc taatccaagt
     1981 ccggttcctt ttgcgggttc agtttttgat ttaacatatt tttcaaatat tctgttttgt
     2041 tcctgcacag atatacccgg tccatcatct tttacagaaa tacgtataaa ttgattttct
     2101 tgtgttgcct cgattgaaat cgtccctttt tttgatgaat atcttatggc gttacttaat
     2161 aaatttacaa c
//
LOCUS       DASWBY010000087         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_78538, whole genome shotgun sequence.
ACCESSION   DASWBY010000087 DASWBY010000000
VERSION     DASWBY010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78538"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VF870_01790"
     CDS             <1..286
                     /locus_tag="VF870_01790"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559617.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEX9250937.1"
                     /translation="KHRTKFKIEVETKNLNEVEEALATKADVIMLDNFEIEDMRKAVK
                     LINGKCKVEASGGVNLETVKQIAETGVDFISVGSLTHSVKALDISLEITI"
     gene            293..1747
                     /locus_tag="VF870_01795"
     CDS             293..1747
                     /locus_tag="VF870_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX9250938.1"
                     /translation="MKEKLKELTKDTAIYGISTMLGRFLNFLLVPFYTNIFLPSEYGV
                     VNILYSYIAIFNIIFIYGMDSAFLKYAAFKDIGDEKENFSTPYISVLISSVLLVALIL
                     LFKNPIAVFLDVGESQTYLIFLSALILFFDSNVVIPFLKLRLERKAKFFSLLRVANIF
                     INIILNVYLIVKLKWGIEAIFISNLAASVFTLLFIFPTIVKNFRFTFHKVLFKRLLKF
                     GLPYLPAGIAVMLVQVIDVPILQKLTDLKTVGIYKANYKLGIFMMLFVNMFQFAWQPF
                     FLNNAKEENAKEIFSKVLTYFTIVGSLMLVLLSLFISDIVKINIAGFSIIGEKYWAGL
                     YFVPVILLAYLFNGLYVVFSAGIYIEEKSIYAPVVAGAGAFTNVVSNYILIPQLNIMG
                     AALATLLSYAVMAAGYYLVTQKYYKINYDYYNLFKIAIGVTFIGIVYYLLMYGGFLNI
                     YYKLILALIFISYILMNVFEKNEMNFIKTKLLRR"
     gene            1748..2206
                     /gene="dut"
                     /locus_tag="VF870_01800"
     CDS             1748..2206
                     /gene="dut"
                     /locus_tag="VF870_01800"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX9250939.1"
                     /translation="MSDEIKIKVKRLSEDYLDVPLPHYATTGSAGMDVRAAIKNDVII
                     EPGKVELIPTDISVEIPSGYEIQVRPRSGLAANHSIGILNSPGTIDSDYRGEVKIILM
                     NFGKEDFKVSKGDRIAQLIVSKVYSAKIEEVNDLNSSHRGEGGFGHTGKK"
BASE COUNT          787 a          324 c          406 g          804 t
ORIGIN      
        1 aaagcatcgc accaaattta agattgaagt ggaaacaaaa aatctgaatg aagttgaaga
       61 agctttagca acgaaagcag atgtaattat gttggataat tttgaaatcg aagatatgag
      121 aaaagccgta aaacttatta atggtaagtg taaagttgaa gcttctggcg gagtaaattt
      181 agaaacagtt aagcaaatag ccgaaacggg tgttgatttt atttctgtcg gctctttaac
      241 acacagtgta aaagctcttg atatctcact tgagataact atctagattc gaatgaaaga
      301 aaaattaaaa gagttaacaa aggatactgc tatttacggc atcagcacga tgctgggcag
      361 gtttttaaat ttccttctcg ttccatttta tacaaatata tttttacctt cagagtacgg
      421 tgttgttaac atactatatt cttatatagc aattttcaat atcatcttta tctacgggat
      481 ggattctgca tttttaaagt atgcagcatt taaagacatt ggtgatgaaa aggaaaattt
      541 ttcaacacct tacataagcg ttcttataag ttcagttctt ttggttgcac taattttact
      601 tttcaaaaat cccatagcag tatttcttga tgtcggagaa agtcagacct atttaatttt
      661 tctatcagcg ctgatactat ttttcgattc caatgttgta attccgtttt taaaattaag
      721 acttgaacgc aaagcaaagt ttttttcact gttaagagtt gcaaatattt ttattaatat
      781 cattcttaat gtatatctga ttgtaaaatt aaaatgggga atagaagcaa tattcataag
      841 taatctggcc gcttcagttt ttactctgct ttttatattt ccaacaatcg ttaaaaattt
      901 tagatttaca tttcataagg tactctttaa acgattatta aaattcggct tgccatatct
      961 tccagcgggt attgctgtaa tgttagtgca ggttatcgat gtaccaatac ttcagaagct
     1021 tacagatcta aaaacggttg gtatttataa ggccaattat aagctcggca tttttatgat
     1081 gctttttgta aatatgtttc agtttgcctg gcagccgttt tttcttaaca acgcaaaaga
     1141 agaaaatgca aaagaaatat tttcaaaggt gcttacttac tttacaatcg ttggaagttt
     1201 gatgctggtt cttctctcac tcttcatttc cgatattgta aaaataaata ttgctggatt
     1261 ttctataatc ggagaaaaat attgggccgg tttgtacttc gtacctgtga tattgctggc
     1321 ttatttgttt aacggtttat acgtggtttt ttcagctgga atatacattg aggaaaaaag
     1381 tatttatgct ccggttgtag caggtgcagg tgcatttaca aacgttgtat ctaactatat
     1441 tctgatccca caactaaata ttatgggtgc agcgctagca actcttctaa gttatgccgt
     1501 tatggctgcg ggatattatc tcgttacaca aaagtactac aaaatcaatt atgattatta
     1561 taatctcttt aagattgcaa taggggttac atttatcgga atagtttatt acttattaat
     1621 gtatggcgga tttttgaata tctattataa attaattctt gcactcattt ttatctctta
     1681 catattaatg aatgtgtttg agaaaaatga aatgaatttt ataaaaacaa aattattacg
     1741 gagataaatg tctgacgaga tcaaaataaa agtaaaaaga ttaagtgaag attacttaga
     1801 tgtcccgctt ccgcattacg caaccacggg aagcgcagga atggatgttc gtgcagcaat
     1861 aaaaaatgat gtaattattg aaccaggcaa agttgaatta attccgactg atatttctgt
     1921 tgaaattcct tctggatatg aaatacaagt aagaccaaga agcggattgg cagcaaatca
     1981 tagcatagga attctaaact ctcccggaac tattgattct gattatagag gcgaagtgaa
     2041 aataatttta atgaattttg gcaaagaaga ttttaaagta tcaaaaggtg atagaatcgc
     2101 tcagttaatt gtgtcaaaag tttattctgc aaaaattgaa gaagtaaatg atttaaattc
     2161 atcacatcgt ggtgaaggtg gatttgggca tacggggaaa aaataatttt aaaattaaaa
     2221 gatgaaaaaa ttgaaaaatt ctaattttta ataaatcaaa ttctatcctt gtttgcttta
     2281 aaattatttt actgtcaaga gctttataaa taaaattaat t
//
LOCUS       DASWBY010000088         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_78625, whole genome shotgun sequence.
ACCESSION   DASWBY010000088 DASWBY010000000
VERSION     DASWBY010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78625"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..583)
                     /gene="greA"
                     /locus_tag="VF870_01805"
     CDS             complement(107..583)
                     /gene="greA"
                     /locus_tag="VF870_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX9250940.1"
                     /translation="MSAGNFVYLTRERLLELEKELQEMKTNGRKKMAAKIAEARAHGD
                     LSENAEYDAAKEEQGLFELKISKMEDTLSRARVIDTSKMATDEVHLLSTVKIKNLKSK
                     KIVEYLLVSPEEADFQEGKISITSPVGQGLIGAKLGDKVKVKAPAGLLEFEIMEIK"
     gene            923..1681
                     /locus_tag="VF870_01810"
     CDS             923..1681
                     /locus_tag="VF870_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9250941.1"
                     /translation="MKPAIKLILKNILIFLFIAGLSSTTLYAQNNEQQLSKSNLKIFV
                     EYRLAKENLLNNNNIKVEITGNKIILSGTVPTLFDKNRAEAEAHSVDENYIIENNLNV
                     EVPIVADSVLSSNIMNKIQSNLFYSIFDWITVTSNNGLVTLNGWVHLPWLRNQFQSEI
                     EKIPGVKSINNKIQNTFGPGELGVRAARLIYNDPMFHGLQYSVNPPIHIIVNNGTVIL
                     DGTVDSEAESSWAANIINFHTDAFSVENNLQIIN"
     gene            1731..>2157
                     /locus_tag="VF870_01815"
     CDS             1731..>2157
                     /locus_tag="VF870_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250942.1"
                     /translation="MKKLKALALLFILSFYAAYANPIISPDNTLLKYYSALLTLNLNH
                     AMDASRIIYHLSMEPSFDKNFLENELNKIQQNVEYANTNIANIILNTLDSKKQEIDKP
                     LKNIDKHLAQVLVDIKEIRIKLNDSQSVTRDLSDIYFQIN"
BASE COUNT          769 a          359 c          318 g          711 t
ORIGIN      
        1 acaagcggat ttggactaac gctgccttcg cctttttttg ctatctcaat cgtaagttgt
       61 atgtatgatt cgtcagccaa aattgtaaca attaattata aaataattat ttgatctcca
      121 taatttcaaa ttccaataaa ccggcagggg ctttaacctt aactttatca ccaagtttag
      181 cgcctatcaa tccttgccca acaggcgatg taatagaaat cttaccttcc tgaaaatctg
      241 cttcttcggg tgatactaaa agatattcaa ctattttctt tgattttaga ttcttaatct
      301 taaccgtgga aagcaaatgc acttcatccg ttgccatttt ggatgtatca ataactctag
      361 ccctggaaag tgtatcttcc attttgctga tctttaactc aaataaaccc tgctcttctt
      421 tagctgcatc atattcagca ttttcagaaa gatcaccgtg tgcgcgtgct tcggcaattt
      481 ttgcggccat tttctttctg ccgttagttt tcatttcctg cagttctttt tcaagctcaa
      541 gcagtctttc tcttgttaga taaacaaaat ttccagcgga cataataaat ctctgtttgt
      601 taatgattta aaaaaaattg ccaccgttcc gataaatcag aacagtggcg ggacaaaaat
      661 aagaattgaa tgcttggatg tcaagaaagg aatattttaa taggctttat ttcttaaaat
      721 tatgcttcct tataaatcaa ccaaccaata attaaaaaat cagttctatt gttttaacca
      781 acagattaat tagctgcgat atgctaaaaa tcacattatt aagtaaactt atatactcta
      841 aggtatgtct aaatcagcaa atcttaatta ataattaacc acaatacatt tataatgaat
      901 aaactaattt aaagtgaata aaatgaaacc cgcaattaaa ctgatattaa agaatatctt
      961 aatttttctt tttatagcag gtctttcctc cacaacctta tatgctcaaa ataatgagca
     1021 gcaattatcc aaaagtaatt tgaaaatttt tgttgaatac aggcttgcaa aagaaaattt
     1081 acttaataat aacaacatca aagtcgagat aaccgggaat aaaattattc tttctggtac
     1141 agtcccaact ttatttgata aaaatcgcgc tgaagctgaa gcgcattctg ttgacgaaaa
     1201 ttacataatt gaaaacaatc ttaatgtaga agttccaata gtagcggatt cagtactctc
     1261 cagcaacatt atgaataaaa tccagtccaa tcttttttac tcaatttttg attggataac
     1321 agttacaagc aataatggtt tagtaacttt aaatggatgg gtccatctgc cgtggttaag
     1381 gaatcaattt caatctgaaa ttgaaaaaat tccaggggta aaaagtatta ataataaaat
     1441 acaaaacacg tttggaccgg gtgaacttgg cgtaagagca gcaaggttaa tatataatga
     1501 tccaatgttt catggattgc aatattctgt aaatccaccg atacatatta ttgtaaataa
     1561 tggaacagta attttagatg gcacggttga ttcagaagca gagagcagtt gggctgcaaa
     1621 tattattaat tttcatactg acgcattttc agtagaaaat aatttacaaa taataaatta
     1681 agatttatgc ggataacact ttaataaaaa ttatttcaag gagatcaatc atgaaaaagt
     1741 taaaagcatt ggcattactt tttattctaa gtttttatgc agcttatgcg aatccgatta
     1801 tttcaccaga caatacgctt ttaaaatatt actctgcatt actcacactg aatttaaatc
     1861 acgcgatgga tgcttccaga attatttatc atctttctat ggagccatca tttgataaaa
     1921 attttttaga aaatgaatta aataaaattc aacaaaatgt tgagtatgca aacactaata
     1981 tcgcaaatat tattttgaac actttggaca gcaagaagca ggagatagac aaaccattaa
     2041 aaaatataga taaacacctg gcacaggttc ttgttgatat taaagagatc agaattaaat
     2101 taaatgattc ccaaagcgtt accagggatt tatcagatat ttactttcaa attaata
//
LOCUS       DASWBY010000089         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_116361, whole genome shotgun sequence.
ACCESSION   DASWBY010000089 DASWBY010000000
VERSION     DASWBY010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116361"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VF870_01820"
     CDS             complement(<1..442)
                     /locus_tag="VF870_01820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9250943.1"
                     /translation="MEVNDPNPIWLNQSHPNYLRWKRARELSIERGKFVKTIVNQEIN
                     TERLSILDLGSGEGGTAEVFSENNFVVSLDLSLIRLQRQISFVISKEGSYPTEKSSTI
                     NETRFLSKFIYISSGFEMTNPELVNGSALQLSFPDQTFDLIIIQD"
     gene            complement(433..1416)
                     /locus_tag="VF870_01825"
     CDS             complement(433..1416)
                     /locus_tag="VF870_01825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9250944.1"
                     /translation="MTFKPKLSLIIAAKNEEKNINSLLDSLEQLNYPIENYEVIFVDD
                     NSIDKTAEFIQLRIADKKNFILIKADKKEIDGKKGALSIGIKKAANNFIVITDADCKA
                     ESNWLNEIASALDYGYDFVFGVAPIRAGKALVEKLSAFENLRNTYLTIAAVGLNIPYS
                     AAARSFAFRKTSFERIGGYSNTTETLSGDDDLLLREGVKRKMLIGTIIEPDALVYSSA
                     PKTFNEYFQQKKRHLQTAFHYLFKQKIFLGFWHLINLISLLSIVIGIISPILALPFAV
                     KLIYDFFVVIKHQNKLGHNFKFYEIFYLQFLFEIFIVINFFNSLSGNNKWK"
BASE COUNT          642 a          317 c          252 g          660 t
ORIGIN      
        1 catcttgaat tataattagg tcaaaagtct gatctggaaa ggagagttga agagcgctgc
       61 cgttaactaa ttctggattt gtcatttcga atcccgaaga tatgtagata aacttcgaga
      121 gaaatctggt ttcattaata gttgaagatt tctcagtcgg gtaggaaccc tctttcgaaa
      181 tgacaaaact gatttgtctc tgcaagcgaa ttaaactcaa atccagactt acaacaaaat
      241 tattttcaga aaaaacttct gctgttccac cctccccgga acctaaatcc aaaattgata
      301 atctttcagt attaatttct tgattaacaa ttgtctttac aaacttacca cgctcaatcg
      361 aaagttctct tgccctcttc catcttaaat aatttgggtg ggattgattt agccagatcg
      421 gattaggatc attcacttcc acttgttgtt acctgataag gaattgaaaa aattgataac
      481 aataaaaatc tcaaacaaaa attgcaaata aaatatttcg taaaacttaa agttatgtcc
      541 taatttattt tgatgtttta tcacaacaaa aaaatcataa ataagtttaa cggcaaatgg
      601 caaagccaat attggcgaga taataccaat cacaatcgaa agtagtgaaa tcaaattaat
      661 caaatgccaa aatcccaaaa atattttctg cttgaaaaga taatgaaacg ctgtctgtaa
      721 atgtctcttc ttctgttgaa aatattcatt aaaagttttt ggtgcagatg aataaacaag
      781 tgcatcaggt tcaatgattg tcccaataag cattttcctt ttaacacctt ctcttaaaag
      841 taaatcatcg tcaccggata gagtctctgt ggtgtttgaa tatccgccga ttctttcgaa
      901 agatgttttt ctaaatgcaa aacttcttgc agctgctgaa tatggtatat tcaaacctac
      961 tgctgctatt gttaggtaag tgtttcttaa attttcaaat gcagataatt tttcgactaa
     1021 tgcttttcct gcccgtatag gtgctacacc aaaaacaaaa tcgtatccat aatccaatgc
     1081 gcttgctatt tcatttaacc aattgctttc tgctttgcaa tcagcatctg ttataacaat
     1141 aaagttattg gctgcttttt ttatcccgat acttagagca ccctttttac catctatttc
     1201 tttcttgtca gcttttatca aaataaagtt ttttttatct gctatcctta attgaataaa
     1261 ttcagcagtt ttatctatcg aattatcatc tacaaaaatt acttcataat tctcgattgg
     1321 ataatttaat tgttcaagcg aatccaaaag tgaattgata tttttttctt cgttctttgc
     1381 tgcaataata agagacaatt ttggcttgaa tgtcaattta ctttttcggt ttaattgata
     1441 gacaaatccc ttataaatgt taattgcaaa aagcaaaaag aaaattatta taatgtatgt
     1501 cagatttgac gcatccaaaa atttaagagc ctttataaga aaaatttaaa atattttaat
     1561 acacatatag taaattaaat tattttcatt taagttaagc caaaataaat actaaacttt
     1621 tacatacaga aaaaattgct gaatattcga agcgtgattt caattctatt tatcacttct
     1681 acgatatatt gtagctaaga aaaaatattt gatgatttta actaaaccct actgtaatca
     1741 aaaattaatt tttcccctaa ctaatcataa ttattcacct agaatggagt tataatgaaa
     1801 aaagcctttc tattattgct tgtttctatt tttgttcttg gactcttttc agcatcaaca
     1861 aatgctcaag t
//
LOCUS       DASWBY010000090         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_41126, whole genome shotgun sequence.
ACCESSION   DASWBY010000090 DASWBY010000000
VERSION     DASWBY010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41126"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="VF870_01830"
     CDS             <1..1761
                     /locus_tag="VF870_01830"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9250945.1"
                     /translation="LMKNLLLIFTLLLMFVGCKQQPEKAAATIYYGGDIVTMEGDSPQ
                     YAEAVAVKDGKILFVGSKDEAMKFEGRDTELLDLQGKTMFPGFIDGHCHFGGFGAQAI
                     AANLLASPDGQVNDIDALVNTLKEWAKGPDLERIGWILGMGYDDAVLKEGRHPTKEDL
                     DKVSTEIPVIVVHISGHFAVMNSAGLKAVGITAETKNPEGGVIRRMPGSNEPNGVLEE
                     LAAIPIMMKIITPKTEENVFYFLDKAQDLAMQFGYTTAEEGRAFGNHEDFMNYAEKKG
                     FKIDVVSYLDYSMENKVKGLSCSADMEIHEVTGESFQRMESPWTGKKYTNGYRVGGMK
                     ITLDGSPQGRTAWSTQPYLIPPDGQKEGYRGYPAIPDDKIVQRLFEKAFQQNWQVEVH
                     TNGDAAIDQLIRTMRPAAEKYGNNDRRIVMIHGQFLRRDQYKDLKELKIIPSMFTMHT
                     FYWGDWYKKIIGPERAQLICPAKSLIDDGFKITIHTDAPVALPNLMQIVWASVNRVSR
                     SGDVMGPDERITPYQAMQAITIWSAWQHFEEDKKGSIKQGKLADLVILSDNPLKINPM
                     KINKISVEATIKGGKTVYKK"
     gene            1854..2372
                     /locus_tag="VF870_01835"
     CDS             1854..2372
                     /locus_tag="VF870_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9250946.1"
                     /translation="MKNSNQTQRKSIRLKEYDYSFPGWYYVTICTKDFIPWFGKINNG
                     KVEYNSLGNIAVKFFEEIPKHFKNTEIDEYIVMPNHVHGIIIINDVVGTRDRVSLRKF
                     GSTDKCSLSIIMNQYKGSITRIAHKNGFDKFAWQPRFYEHIIRNDIDLQRIRTYIRNN
                     PLKWELDVYNNT"
     gene            2856..>3067
                     /locus_tag="VF870_01840"
     CDS             2856..>3067
                     /locus_tag="VF870_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250947.1"
                     /translation="MKSTLDFIKTMIIGGIIFFIPLIILIVVIQKAFQIASVLVAPII
                     NLLNITDIFGIGAEIVISIIIMLFLL"
BASE COUNT         1062 a          468 c          612 g          925 t
ORIGIN      
        1 ttaatgaaaa acctactatt aatttttaca ttattattaa tgtttgttgg ttgtaaacaa
       61 caacctgaaa aagctgctgc cacgatatac tatggcggtg atattgttac tatggaaggt
      121 gactctccac agtatgcaga agcagtagca gttaaagacg gcaaaatact ttttgttggc
      181 tcaaaagacg aagctatgaa atttgaagga agggatactg agctacttga ccttcaggga
      241 aaaaccatgt tccccggttt tatcgatggc cattgtcatt ttggtggttt tggtgcacag
      301 gctatagcag caaatttatt ggcttcacct gatggacagg taaatgatat tgatgctcta
      361 gtcaatacat taaaagaatg ggcaaaagga cctgatctgg aacgaatcgg ctggattttg
      421 ggaatgggtt atgatgatgc tgttttaaag gaaggcaggc atcctacaaa agaagatctg
      481 gataaagttt caaccgaaat tccggttatt gttgttcata tttccggaca ttttgccgta
      541 atgaatagtg cgggattgaa agcagtcggc ataactgctg aaacaaaaaa tcctgaaggt
      601 ggtgtgatca gaagaatgcc gggcagtaat gagccaaatg gtgttttgga ggaacttgcc
      661 gctatcccga ttatgatgaa aataattaca cctaaaactg aagaaaatgt tttctatttt
      721 cttgataagg ctcaggacct cgccatgcaa ttcggctaca cgactgcaga ggaaggacgt
      781 gcttttggaa accacgaaga ttttatgaac tatgctgaaa agaagggatt taaaatcgat
      841 gtagtttcat atctcgatta ttcaatggaa aataaggtta aaggattgag ctgctctgct
      901 gatatggaaa ttcatgaggt aacaggagaa tcctttcaga gaatggagag tccctggaca
      961 ggtaaaaaat atacaaatgg ttatcgtgtg ggaggaatga aaatcactct tgacggttca
     1021 ccacagggaa gaacagcatg gtccactcag ccttatctga ttccaccaga cggacaaaaa
     1081 gaaggttaca gaggttaccc cgcaattccg gatgacaaaa ttgtgcaaag attatttgaa
     1141 aaagcatttc agcaaaactg gcaggttgag gttcacacca atggagatgc tgcaatagac
     1201 cagctgataa gaacaatgag accggctgct gaaaaatatg gaaacaacga ccggagaatt
     1261 gtaatgatac acgggcagtt tttaagaaga gatcaataca aggatttaaa agaattgaaa
     1321 attattcctt cgatgtttac catgcataca ttttactggg gtgattggta caaaaaaatc
     1381 attggtcctg aaagagctca gctaatctgt ccggcaaaat cgctgatcga tgacggtttc
     1441 aaaatcacta ttcacaccga tgcacctgta gccctaccca acctgatgca gattgtttgg
     1501 gcaagtgtta accgggtttc gcgatctggt gatgtaatgg gacctgatga aagaatcact
     1561 ccttaccagg caatgcaggc aattactata tggagcgcct ggcaacactt tgaggaagat
     1621 aaaaaaggaa gtattaaaca aggtaaactg gctgacttag tcatattatc agacaatccg
     1681 ttaaaaatta atccgatgaa aataaataaa attagtgttg aagcaacaat aaaaggaggt
     1741 aagacagttt ataaaaaata attgtattgt ttaaatattt ctggaaagtt attgatataa
     1801 aaaatgattg gaataaaatt tatccataaa atactatatc taatcatatt gtagtgaaga
     1861 attcaaatca aactcaacgt aaatccatta gattgaagga atatgattat tcttttccgg
     1921 gttggtatta cgttactatt tgcacaaaag attttattcc gtggtttgga aaaattaata
     1981 atggtaaagt tgagtacaat tcattaggaa atattgcggt taaatttttt gaggagattc
     2041 caaaacattt taagaacact gaaattgatg aatatattgt tatgccaaac cacgtgcacg
     2101 gaattataat tatcaacgat gttgtaggga cacgcgatcg cgtgtcccta cgaaagtttg
     2161 ggagtacgga taaatgttca ctttcaatca taatgaatca atacaagggt tctattacaa
     2221 ggatcgctca caaaaatggt ttcgacaagt ttgcatggca gccccgtttc tatgagcata
     2281 taataagaaa tgatattgac cttcagcgta taagaactta tataagaaac aatcccctca
     2341 agtgggaact tgatgtatat aacaatacct aattccgagg gctcgactgt tgttgatgtg
     2401 aaaaatgatt ggtataaatt ttatccagag aaatagttac atacgagttt attgatcgga
     2461 atatgatgtc cgagattaga aatttattta aaggcgttga agtgattcag cgcctttttt
     2521 attttccccc aaaaaaacaa ctatatcata aactaatctg taattaatgc tgtatttttg
     2581 ttttgaataa ttaacgaatt aaatgaagac tatattattc gaaaattact tatctagctc
     2641 aagcctggca tctgcgccaa tacaagaatt ttatatcacc taaaaactaa taccaattaa
     2701 tggagaggaa gaaataaaat tattttgact gcttcaaaaa ttattataaa attttttaca
     2761 cctgttctta aagttttaac tttttaagtt gagtttgctc gtaataaata aaattgttag
     2821 aattaaaaat attttgattg aaaggactgt tagttatgaa aagtacatta gattttatta
     2881 agactatgat aattggcgga ataattttct ttataccact aattatctta attgttgtaa
     2941 ttcagaaagc atttcagata gcatctgtcc tggtagctcc gattattaat ttattaaaca
     3001 taacagacat ttttggaatt ggagctgaaa ttgtaatctc aattattatt atgctttttt
     3061 tacttta
//
LOCUS       DASWBY010000091         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_191491, whole genome shotgun sequence.
ACCESSION   DASWBY010000091 DASWBY010000000
VERSION     DASWBY010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191491"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VF870_01845"
     CDS             complement(<1..1589)
                     /locus_tag="VF870_01845"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX9250948.1"
                     /translation="MVDEATMVKPNMVHDVHCLRHTKSKKLRTISRLLRAINIISLLI
                     GLVAITSISDSFAQDSQRRPNIVIILADDMGYSDMGSFGSEIQTPNLDKLAYSGVRFT
                     NFYTHATCSPTRSMLLSGVDTHLNGLGNMDEWSAPNQNGVKGYEGHLNKEVTTLPLLL
                     NQAGYHTYMVGKWHLGKAPDLIPAARGFERDFALLDGAGSYWDMTNLAALTPKSVFTE
                     DGKYLTELPDDYYATKTYTDKLIGFIEDNRKDGKPFFAYVAEQAPHDPYHLPRDWRNR
                     HVGEYDKGWDQVREERLKKQIELGIVPANTQLAERMWFLPDPFVLAPAAKAILGKKME
                     LYAGMVENMDYHIGRLIDYLKQIGEYENTIFIVFGDNGAEGTDLFKMIAGTPGSLNYL
                     FAATNWSETDPNAWGDPGSYVAYGPMWAQVSMTPFSQYKGWMGEGGIHNALIVSGPVI
                     KRPAGSINNGQMHVLDIMPTLLEIANVKYPEKSEDGSELHPLIGKSWVNVLTGKSESP
                     RTKQDYLAWEIFGNRALIQGD"
     gene            complement(1711..1992)
                     /locus_tag="VF870_01850"
     CDS             complement(1711..1992)
                     /locus_tag="VF870_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250949.1"
                     /translation="METVTINSRIKKNNKTELYQTLESLKPYIKSHCKDLELKINPDY
                     SLNMHITFDDQESLENNFYNYEFKILKGTLKSLCEDVVIKINDTVVIDN"
     gene            complement(2182..>2973)
                     /locus_tag="VF870_01855"
     CDS             complement(2182..>2973)
                     /locus_tag="VF870_01855"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9250950.1"
                     /translation="RAIHKNSKRSKKPFIKINCASIPENLIESELFGHEKGSFTSAFE
                     KRIGRFEIANGGTIFLDEIGELPVSLQSKLLSVLQQGEFERIGSSKTIKTNVRVIAAT
                     NKILEDEIKKGNFRSDLYYRLNVFPISITPLRERKSDIITLAEYYTRIYSEKFGKSIK
                     AISKKSIKQMMDYSWPGNVRELENVIERAIITSQNKILNIEYLPALEIPTNEFISLDE
                     NERIHILKALEKTHWKINGKGGAAELLKKNPQTLRARIKKLKIER"
BASE COUNT          858 a          587 c          485 g         1043 t
ORIGIN      
        1 caatctccct gaattaatgc acggttccca aaaatttccc aggcaagata atcctgcttg
       61 gttcgaggtg attctgattt accagttaat acatttaccc acgatttacc gatgagtgga
      121 tgcagttcac tgccgtcttc agatttctcg ggatatttaa catttgcaat ttcgagtaaa
      181 gttggcatta tatctaaaac gtgcatctgc ccgttgttaa tgcttccggc aggtcgttta
      241 atcacaggac cgctaacgat tagagcatta tgaataccac cttcacccat ccaacccttg
      301 tactgactaa atggcgtcat tgatacctgt gcccacatcg gaccataagc aacatagctg
      361 ccaggatcac cccatgcatt cggatcagtt tctgaccaat tcgttgctgc aaaaagataa
      421 ttaagactac ccggtgttcc ggcaatcatt ttaaaaagat cagttccctc agctccatta
      481 tcgccaaaca caatgaagat agtgttctca tactctccaa tttgtttaag gtaatctatt
      541 aatctgccta tgtgataatc catattttca accataccgg cgtagagttc catcttctta
      601 ccaagaattg cttttgctgc cggagcaagt acaaaaggat ccggaaggaa ccacattctt
      661 tcagcaagct gagtattggc agggacaatg ccaagttcaa tttgcttttt cagacgttct
      721 tctcgaacct gatcccagcc cttatcgtat tcaccaacat gccgattgcg ccagtctctc
      781 ggaagatgat atgggtcgtg cggtgcttgt tcagcaacat aagcaaaaaa tggtttgcca
      841 tcttttctgt tatcctcgat aaatccaatt aacttgtcag tgtaagtttt ggttgcgtag
      901 taatcatcag gaagttctgt aagatatttt ccatcctccg taaaaactga ttttggagtg
      961 agtgctgcta agtttgtcat atcccaataa cttccggctc catccagcaa tgcaaagtca
     1021 cgctcaaaac ctctggctgc aggtatcaga tcaggagctt ttcccagatg ccatttgcca
     1081 accatatatg tatgataacc agcttggtta agcaaaagag gcaaagtagt aacttctttg
     1141 ttcaggtgac cttcataacc tttcacacca ttctggttag gtgcagacca ttcatccata
     1201 tttccgagac catttaagtg agtgtcaaca ccgcttaaca gcattgatct tgttggtgaa
     1261 caagttgcgt gtgtatagaa gttagtaaag cgtacacctg agtatgccag cttatctaaa
     1321 ttcggagttt gaatttcact gccaaatgaa cccatatcag aataacccat atcatcagca
     1381 agaattatca ctatgtttgg tctccgctgc gaatcttgtg cgaaactatc agatattgag
     1441 gtaattgcga ccagtcctat aagaagagaa attatgttaa ttgctctgag taaccggcta
     1501 atggttctca gtttttttga tttggtgtgc ctcaaacaat gaacatcgtg taccatatta
     1561 ggcttcacca ttgttgcttc atcaaccata ttagaactcc ttcttttatt gagcttgatt
     1621 taatactatc atttgtaata ctactattta acattttttt cgagcaatta taattttcta
     1681 attcaattat ccatttctaa tcattaattt ttaattatca attacaacag tgtcatttat
     1741 tttaattaca acatcttcac aaagactttt aagtgtaccc ttaagaattt taaactcata
     1801 gttataaaag ttgttttcca aactttcctg gtcatcaaag gtgatatgca tattcagact
     1861 ataatccgga tttatcttta actcaagatc tttgcagtga gattttatgt acggcttaag
     1921 tgattccaat gtttgatata attcagtttt attatttttt tttatccggg aattaatggt
     1981 aactgtttcc attgcaacac ctgccaaata ttgatgatat tattattttg gttctaaaat
     2041 gattaatatg caataacaaa ccgctggcca gatataaata acttgaaaat aatgtaaatt
     2101 ctttgttttt atataatttt agaaaagaat attaccaaat atggagaata tcgctaaata
     2161 tggagagatt ttatgaatgc cttaacgctc gatcttaagc ttttttatcc ttgctcgtaa
     2221 ggtttgagga ttctttttta ataattcagc agcgccgcct ttaccgttaa ttttccaatg
     2281 agttttttcg agggctttta aaatatgaat tctttcattc tcgtcaagtg aaataaattc
     2341 attcgtggga atctctaacg caggtaaata ctcgatgttt aatattttat tctgtgatgt
     2401 gattatagct ctctcaataa cattttccag ttcacgtacg tttcccggcc aggagtaatc
     2461 catcatttgt tttatagatt tttttgaaat tgcctttatt gatttgccaa atttttccga
     2521 atagattctt gtataatatt ctgccagtgt aattatgtct gattttcttt ctcttagcgg
     2581 agtaattgaa atagggaaca cattaagtct ataataaaga tcgctacgga aatttccttt
     2641 tttaatttca tcttcaagaa ttttattggt tgcggcgata actcttacgt tcgtttttat
     2701 cgttttagaa cttcctattc tttcaaattc accctgctgc aaaacgctca atagtttaga
     2761 ttgaagagaa actggtaatt cgccaatttc atctaaaaat attgtaccgc catttgctat
     2821 ttcaaatcta cctattcttt tttcaaaggc acttgtaaat gagccctttt catgaccaaa
     2881 taattcactc tctattaaat tctcaggaat agatgcacag tttattttaa taaatggttt
     2941 cttacttctt ttactatttt tatgaattgc tct
//
LOCUS       DASWBY010000092         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_717856, whole genome shotgun sequence.
ACCESSION   DASWBY010000092 DASWBY010000000
VERSION     DASWBY010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717856"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VF870_01860"
     CDS             complement(<1..899)
                     /locus_tag="VF870_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9250951.1"
                     /translation="MSDLEYLKQRKSYDEKAKSAKTNGMKFTTVSGQDINVFYGPDDI
                     ENINYLSEIGFPGEYPYTRGIHPNGYRGKIWTMRQFAGFGSPEDTNERFHYLLNHGQT
                     GLSVAFDLPTLMGWDADAPISDGEVGICGVAISSLQDMEILFNKIPLDKVSTSMTINS
                     PAAMIFAFYLAVAQKQGVDFKNLRGTLQNDILKEYIAQKEFIYPPNPSMRIIVDMIEY
                     CKNEVPQWNPVSVSGYHIREAGSTSVQELAYTLADGFAYIEACIERGMDVDEFAPRIS
                     FFFNSHLDFFEEIAKFRAARKIYA"
     gene            complement(946..>1668)
                     /locus_tag="VF870_01865"
     CDS             complement(946..>1668)
                     /locus_tag="VF870_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEX9250952.1"
                     /translation="RERTAVTDEDIDLVFSYLTKDIKPSSGIGAGYEQTNNKFDKQIE
                     HSASNEIETSFLIQLHNKYILSQIKSGLMIIDQHVAHERILYEKVLSRLDANMPFIQQ
                     LLFPIKIQFDVASYEILKELNEIITKLGFKLKYLPKSYVLIEGVPDDIKSGSEEKILR
                     EFISEYLNNQTEKQLEEKDNIAKSYSCKAAIKAGDKLSEKEMRLLIDQLFATSMPYVC
                     PHGRPIVIKISLEEFDRRFGRT"
BASE COUNT          521 a          319 c          250 g          578 t
ORIGIN      
        1 gcataaattt ttctagctgc tctgaatttt gcaatttctt caaaaaaatc cagatgagaa
       61 ttaaagaaaa aagaaattcg tggagcaaat tcatctacat ccattccccg ttcaatgcag
      121 gcttcaatat aagcaaatcc atctgcaagt gtgtatgcaa gttcctggac tgatgttgag
      181 cctgcttcgc gaatatgata tccgctgact gaaacaggat tccattgagg cacttcgttt
      241 ttacaatatt caatcatatc aacaataatc ctcattgaag gatttggagg atagataaac
      301 tctttttgtg cgatatactc tttaagaata tcattctgaa gtgtgccgcg taaattttta
      361 aaatcaactc cctgtttttg ggcaactgca agataaaatg caaaaatcat cgccgcagga
      421 gaattgattg tcattgatgt tgaaacttta tctaatggaa ttttattaaa aagaatttcc
      481 atatcctgta aagatgaaat cgcaacaccg caaattccta cttcaccgtc gctaattgga
      541 gcatcggcat cccagcccat taatgttgga agatcaaatg cgactgataa acctgtttgt
      601 ccgtgattta ataaataatg aaatctttca ttcgtatctt ccggtgaacc gaaacctgcg
      661 aactgtcgca tagtccaaat tttaccacga taaccatttg gatgaattcc acgagtgtag
      721 ggatactcac caggaaaacc gatctcactt aaataattta tattttcaat atcatccggc
      781 ccgtaaaaaa catttatatc ctgcccgctt acagtggtaa acttcattcc atttgtttta
      841 gcagatttag ctttttcatc atagcttttt ctttgcttca gatattctaa atcagacata
      901 gatttccttt ttattgatta agtaaaattg attcctaaat ataaactaag ttcttccaaa
      961 tcttctgtca aattcttcta aagatatttt tatcacaata ggtctgccat gcggacaaac
     1021 gtacggcatt gatgttgcaa ataactgatc aattaataat ctcatttcct tttcggaaag
     1081 tttatccccg gctttaattg cagctttgca ggaatacgat tttgcaatgt tatctttctc
     1141 ttctaactgt ttttccgttt gattgtttag atattcagaa ataaactcgc gtaatatttt
     1201 ttcttctgat ccgcttttaa tatcatccgg aacaccttct atcaacacat aactttttgg
     1261 caaatatttc aatttaaatc caagtttagt gataatctca ttcagttctt ttagtatttc
     1321 ataactggca acatcaaact gaatctttat cggaaaaagt aactgctgaa taaatggcat
     1381 atttgcatca aggcgagata aaactttctc atataatatt ctctcgtgag caacatgctg
     1441 atcaataatc ataagtccgc ttttaatttg agatagaata tatttgttat gaagctgaat
     1501 taaaaagcta gtctcaattt catttgatgc agaatgttca atctgtttat caaatttatt
     1561 attggtttgt tcataacctg ctcctatgcc tgaacttggc tttatatcct ttgttaaata
     1621 actgaaaacc agatcaatat cttcatcagt tactgcagta cgttcccg
//
LOCUS       DASWBY010000093         6912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_529744, whole genome shotgun sequence.
ACCESSION   DASWBY010000093 DASWBY010000000
VERSION     DASWBY010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529744"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VF870_01870"
     CDS             <1..587
                     /locus_tag="VF870_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250953.1"
                     /translation="TVEEKLDASNYSYLKVSEDGNSYWIAVPTMDVKPGEKINYSKFM
                     EMQNFKSETLGRTFESVLFVDDASKGESGQNIASPHSKISTGKDASIKVEPLKDGYSI
                     EQIYNQKSSLVNKVVKVKGVVVKINKGIMGTNWIHIQDGTGKDGTHDLLVTSDTVPEM
                     GNTIIAEGKVIVDKDFGAGYFYSVLLENSKITIE"
     gene            complement(676..1290)
                     /locus_tag="VF870_01875"
     CDS             complement(676..1290)
                     /locus_tag="VF870_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9250954.1"
                     /translation="MSENNILIKIESYVKELFKNRSAAENIYHNILHTTEVVNVAAKI
                     AESENLSAEDTEILLIAAWFHDTGYFHCCKGHEEQSSEYARDYLVREGYPEEKILRII
                     ECIKATQIPHAPTNKLEEIICDADLHHLGMIDIKERGELLRKEFEMNGLKKLSDIEWL
                     KNSLNFFNKHHFFTEYAKRKFGVQKNLNILKMKEQLEILEGLNN"
     gene            1453..1938
                     /locus_tag="VF870_01880"
     CDS             1453..1938
                     /locus_tag="VF870_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9250955.1"
                     /translation="MKNLILLRHAKSSWKDTSLDDFDRPLNNRGERDAPKMADRVARE
                     KIQIDLIISSSALRTSDTAKIFAEIIGYKSRIIFDDNLYEASYKKILDLINLIEDKYK
                     GVLLVCHNPGITNLANYLSDHFIDTIPTTGIVGLSFNCSWKELNENSCSLLFYDYPKK
                     K"
     gene            complement(1991..3928)
                     /locus_tag="VF870_01885"
     CDS             complement(1991..3928)
                     /locus_tag="VF870_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9250956.1"
                     /translation="MQRNGIRMLAAVMFTDMVGYTAMMQEDEEHANSLRNRHRKVLED
                     LTLDHQGRILQYFGDGTLTIFGSAVEAVLCAMKVQIELQKDPKVNLRIGIHSGDIVYD
                     DNGIFGDCVNIASRIEASAVGGSILISRKVYDELSNHKEISAVSLGYHEFKNVKNPIE
                     VYAIKNEHLNIPESTSLETLVGLKKESIAVLPFINMSSDADNEYFSDGMTEEILNALA
                     KVDKLHVTSRTSSFAFKGKNVDIREIGKQLAVKTVLEGSVRKVGNRVRVTAQLIETKS
                     GYHKWSETYERDIEDIFAVQDEIARTISKNLAENLTSADQKKTLVKSSTRNMEAYNLY
                     LKSSHFWKKWTPLDIRKSMEYLEQAIQLDENFAKAYSALSGCYVYLGAFGQMPATIAY
                     PKAKEYANKALQLDHSLPESHLSIAMVEFFDWNWDAAYKSFFKTLELNPNSAEAHQYF
                     AYYMFAIGNNKKAVSESEKALEIDPLSITINTSLAETYAHCGMFDQSKQQFLKTLELD
                     HNFRAALNGLGWTHYFLGECELAIKRLKQSQDLLGDPLKSNAALGYIYAKLGMKSELE
                     NCLKKLEERAKNEKDVSFLFDYAIINMGMKNYDKAFEYLDLAFEQKIGGLIFIRGRYW
                     KEIQDDKRFKKLILKMNLPYN"
     gene            complement(4070..6325)
                     /gene="pta"
                     /locus_tag="VF870_01890"
     CDS             complement(4070..6325)
                     /gene="pta"
                     /locus_tag="VF870_01890"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEX9250957.1"
                     /translation="MKLTKEESLQYHSSERKGKIEVIATKPCLTARDLSMAYTPGVAE
                     PCREIHADVENVYKYTAKGNLVAVLSNGTAVLGLGDIGAEAGKPVMEGKGVLFKRFAD
                     IDVFDIEVNSHDPKEIIRLTQMLEPTFGGINLEDIKAPECFEIEETLKATMNIPVFHD
                     DQHGTAIISCAALINAVEVAGKKLDEVRIVVNGAGASAISCCKHYVRAGAKKENIYMF
                     DSKGCITKDRKDLNKYKDGFAQDKGFPSLEAAFKDADVFIGLSIGNVVKKEWVKTMAP
                     NPIIFAMANPDPEIMYDDAISVRKDLIMATGRSDYPNQVNNVLGFPFIFRGALDVRAT
                     QINDEMKMAATLALAKLAKEKVPEMVLQAYGGEDFEFGKEYIIPKPFDPRVLWYVAPA
                     VAKAAIETGVAKVKEMDWVLYEEQLKERLGLSKEIVRVMIHKAMKNPKKVVYPEGEEE
                     NIIRAAHTVYSDGVANPILLGNEKIIKSEIERIGYDVSEFQIIDPETSEKCDTYALEF
                     FKKRQRRGATLWDAKNLIHKPNYFGAMMVEVGAADALISGQTTSYPNTIKPALQCIGV
                     KEGFKIVSGMYIVITKKETYFFADCTVNVNPNAEQLAEIAISAASVVKAFDVVPKIAM
                     LSFSNFGSAAYPESIKVKEAVKIVKSKMPELIIDGEMQADTAVVPEILDKIFPFTSIK
                     GGANVLVFPSLDAANIAYKLMARIGQATVIGPILMGMKKPVHVLQRGATVDDIYNMTA
                     IAVVDAQTTKK"
     gene            complement(6635..>6912)
                     /locus_tag="VF870_01895"
     CDS             complement(6635..>6912)
                     /locus_tag="VF870_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX9250958.1"
                     /translation="SFTGSSKIWLRATGSTLVSQLVDSFVVLFIAFYLGAGWDLKLVL
                     VIGLVNYIYKFIIAIIVTPALYLLHAIIDKYLGKELSEHLQAEAMTK"
BASE COUNT         2195 a         1278 c         1083 g         2356 t
ORIGIN      
        1 taactgttga ggaaaaactt gatgccagta attatagtta tttaaaagtt tctgaagacg
       61 gaaattcata ctggattgct gtaccaacta tggatgttaa gcccggagaa aaaataaatt
      121 attctaaatt tatggaaatg caaaatttca aaagtgaaac actcggcaga acctttgaat
      181 cagtcttgtt tgttgatgac gccagtaaag gtgaaagcgg acaaaacatt gcctccccgc
      241 actcgaaaat ttccacagga aaggatgctt caattaaagt tgaacctctt aaagatggtt
      301 actcaattga acaaatttat aatcaaaaat catctcttgt taacaaggtt gttaaagtaa
      361 aaggtgttgt agtaaaaatt aataaaggaa taatgggtac aaactggatt cacattcagg
      421 atggaaccgg caaggatggt acacacgatt tgttagtaac atctgacact gttcctgaaa
      481 tggggaacac tattattgct gaaggaaaag ttattgtaga taaagatttt ggtgcaggat
      541 atttttattc cgtactgctt gaaaattcta aaatcacaat tgaataaagt tgataaaaaa
      601 acccgaagaa tatatgatct tcgggttttt gttttttaca agcattagaa ttttcttagt
      661 ggtaaatttt aatttttagt tattaaggcc ttccagtatt tctaactgtt ccttcatctt
      721 tagaatatta agatttttct gaactccaaa ctttctttta gcatattcag taaagaagtg
      781 gtgcttatta aaaaagttta aagaattttt tagccattcg atatctgata atttttttag
      841 accgttcatc tcaaattctt ttctaagtaa ttcccctctc tctttaatat caatcattcc
      901 taaatgatgc aaatcagcat cacagataat ttcttcaagc ttatttgttg gtgcgtgtgg
      961 aatctgagtg gcttttatac attcaattat ccttaaaatt ttttcttctg gataaccttc
     1021 tctaactaaa taatcgcggg catattcaga actttgttcc tcgtgtcctt tacaacaatg
     1081 aaagtatcct gtatcatgaa accaggctgc tattaaaagt atttcagtat cttcagcaga
     1141 taagttttct gattcagcaa ttttagcggc aacattcact acttcagtcg tatgcaaaat
     1201 attatgataa atattctctg cagcggatct atttttaaac agttctttta cataactttc
     1261 aattttaata agtatattat tttcagacat tatatttcct tgcaatagca ctattataca
     1321 ttatcttttt cgattaaatt caacataatt tttacttatt ttatctttaa tttcgatgcg
     1381 aaactatact aattcttaag aaggaatggt gacaattatc acagttgtga tttgaaaaat
     1441 agggcacaaa taatgaaaaa tttaatcctc ttaagacacg caaaatcaag ctggaaggat
     1501 acttcacttg atgattttga cagaccttta aataatcggg gagaacgcga tgcgcctaaa
     1561 atggccgaca gagtagccag ggaaaaaatc cagatcgatt taattatttc gagcagtgct
     1621 ttgcgaacat ctgatacagc aaaaatattc gcagaaatta taggttataa atccagaatt
     1681 atatttgatg ataatcttta cgaagcatcc tacaaaaaga ttttagattt gataaatctg
     1741 atagaggata aatataaggg tgtgcttctt gtctgccata accctggtat tacaaatctt
     1801 gcaaattatc tttcagacca ttttatagat accataccaa caacgggaat agttggcctt
     1861 tcttttaatt gcagttggaa agaattaaat gagaatagtt gttctttact tttctatgat
     1921 tatccaaaga aaaaataatt tgtaaatgaa ttgtttaaaa ctggtaaaaa tttaagttta
     1981 aaagtaaaca ttaattgtaa ggtagattca ttttgagtat taacttttta aaccttttat
     2041 catcctgaat ttccttccag tatcttccgc gaataaatat cagtccaccg attttttgtt
     2101 caaaagcaag atctaaatat tcaaacgctt tatcgtaatt tttcataccc atattaataa
     2161 tcgcgtagtc gaaaagaaaa gaaacatctt tttcattctt tgctcgttct tcaagttttt
     2221 tcaagcaatt ttccagctcg cttttcatac caagcttagc ataaatataa ccaagagcag
     2281 cattagattt taatggatca cctaataagt cctgcgattg tttcaatctt tttatggcaa
     2341 gttcacattc tcctaaaaaa taatgagtcc agcccaaccc attaagcgcg gctctaaaat
     2401 tatgatctaa ttccagagtt ttaagaaatt gttgttttga ttgatcaaac attccgcaat
     2461 gagcataagt ttctgcaaga gaagtattga tagtaataga aagcggatcg atctctaaag
     2521 ctttttctga ttctgaaact gcctttttat tattgccaat tgcgaacata taataagcaa
     2581 agtattgatg tgcctcggca ctatttggat taagctcaag agttttaaaa aaggatttgt
     2641 aagcagcatc ccagttccaa tcaaaaaatt caaccattgc aattgataga tgtgactcag
     2701 gtaatgaatg atcaagctgc agcgctttat ttgcatattc ttttgccttt ggataggcaa
     2761 ttgttgcagg catctgacca aatgcaccaa gataaacata gcagccagaa agtgcagaat
     2821 aggcctttgc aaagttctca tcaagttgaa ttgcttgttc taaatattcc atagatttgc
     2881 gaatatccag cggggtccat ttcttccaga aatgtgatga ctttaaatat aaattgtaag
     2941 cctccatatt tcttgttgaa gattttacta aagttttctt ttgatcagca gatgttaagt
     3001 tttctgcaag atttttagaa atagtgcgag caatttcatc ctgaacagca aaaatatctt
     3061 caatatcccg ttcataagtt tcagaccatt tatgataacc actttttgtc tcaattaact
     3121 gagcggttac ccgaaccctg tttccaactt ttcttacact gccttctaaa actgttttta
     3181 cagctaactg tttgccaatt tctcttatat caacattttt gcctttgaat gcaaaagaag
     3241 acgttcttga ggtgacatgt aatttatcaa cttttgctaa agcatttaat atttcttcag
     3301 tcattccgtc agaaaaatat tcattatccg catctgaact catattaata aaaggtagta
     3361 cggcaatgga ctcttttttt aatccaacaa gtgtctctag tgaagtgctt tccggaatgt
     3421 tcaggtgctc atttttaatt gcatatactt caattggatt cttaacgttc ttaaattcgt
     3481 gataaccaag tgatacggca gaaatctctt tatggttact taattcatca taaacttttc
     3541 tggaaattaa aatcgatcca ccgactgctg aagcctctat tctagaagct atgttaacac
     3601 aatctccaaa aattccgtta tcatcataaa ctatatctcc ggaatgaatc ccaattctaa
     3661 gatttacctt tggatctttt tgtaattcaa tctggacttt cattgcacat aaaaccgcct
     3721 caacggcact tccaaatatt gttaaagtac catctccaaa atactgtagt attctgccct
     3781 gatgatcaag agttaaatct tccagaactt tacgatgtcg attacgaaga ctatttgcat
     3841 gctcctcatc ttcctgcatc attgcagtat agccgaccat atcagtaaac attacagcgg
     3901 caagcattct gatcccgttt cgttgcattt aaaagtcttg aatattattg attaaaaata
     3961 ttgagcaaaa ttagttgcca aggataaaaa agacaattgt tcaatgatgt tcggtgaaat
     4021 tttagggtga acagatcgct caccctaaag agaagaatta cttaaaattt tatttttttg
     4081 ttgtttgtgc atcaacaact gcaattgcag tcatgttata aatatcatct acagtcgcac
     4141 ctcgctgtaa aacatgaacc ggttttttca ttcccattag tattggtcca attacagtcg
     4201 cttgtccaat cctggccata agcttataag caatgttagc tgcatccaaa cttgggaata
     4261 ctagcacatt tgcaccacct ttaattgaag taaacgggaa aattttatca agaatttctg
     4321 gaacaactgc agtatctgcc tgcatctcac catcaattat aagctcaggc attttagatt
     4381 tgactatctt aactgcttct ttaaccttaa tcgattcagg ataagccgca ctgccaaagt
     4441 tactgaatga tagcatagct atttttggaa caacatcaaa tgctttaacc acgctggctg
     4501 cagatatagc tatttctgct aactgctcag catttggatt aacatttaca gtgcaatcag
     4561 caaaaaagta agtttctttt ttagtaataa caatgtacat accagaaaca attttaaatc
     4621 cctctttaac accaatacat tgcagagcag gttttattgt atttggataa cttgttgttt
     4681 gaccacttat tagtgcatct gcggccccca cttctaccat catcgcgcca aagtagttcg
     4741 gtttgtggat aagatttttt gcatcccaaa gagtagctcc tctcctttgg cgttttttaa
     4801 agaattctaa agcgtaagta tcacattttt ctgaggtttc aggatcgatt atttggaatt
     4861 cactaacatc gtaaccgatt ctttcaatct cactctttat aatcttttca tttcccaaaa
     4921 gaattggatt tgctactcca tcactataaa cagtgtgtgc agcacggatt atattttctt
     4981 cttcaccttc cggataaaca actttcttag gatttttcat cgctttgtga atcataaccc
     5041 tgacaatttc tttggagaga ccgagtcttt ctttaagctg ttcttcatat agaacccaat
     5101 ccatttcttt aactttggca actccggttt ctattgcggc ttttgctaca gcaggagcaa
     5161 cataccaaag cactcttgga tcaaaaggtt ttggaatgat atattctttt ccgaattcaa
     5221 aatcttcacc gccataggcc tgtaaaacca tctcgggaac tttttctttt gctaattttg
     5281 caagtgccag ggtagctgcc attttcattt catcatttat ttgtgttgct ctaacatcta
     5341 aagcaccacg gaatataaat ggaaatccta aaacgttatt aacctggttt ggataatcgc
     5401 tgcgaccggt agccataatc aaatcttttc gaacagatat tgcatcatca tacataattt
     5461 caggatcagg attagccatt gcaaagatga ttggatttgg ggccattgtt ttgacccatt
     5521 cttttttcac aacatttcca atggaaagcc caataaatac gtctgcatct ttaaaggctg
     5581 cttccagtga tgggaaacct ttatcttgtg caaagccatc cttgtattta tttaaatcct
     5641 ttctatcctt tgtaatgcat cccttcgaat cgaacatata aatattttct tttttcgctc
     5701 cggcacgaac ataatgttta cagcaagaaa ttgctgaagc acctgctccg ttaacaacga
     5761 ttctgacctc gtctaatttc tttccggcaa cttcaactgc gtttattaaa gcagcacacg
     5821 aaataattgc agttccgtgc tgatcatcat ggaaaacagg aatgttcatt gttgctttta
     5881 atgtttcttc gatctcaaag cattctggcg ctttaatatc ctcgagatta attccaccaa
     5941 aagtaggttc taacatttgc gtaagtctga taatctcttt tggatcgtgc gaatttactt
     6001 cgatatcaaa aacatctata tctgcaaatc ttttaaataa aactccctta ccttccatca
     6061 ctggttttcc tgcttccgcg ccaatatcgc ctaaacctaa taccgcagta ccattagata
     6121 aaactgcaac taaatttcct tttgcagtgt acttgtaaac attctccaca tcagcatgga
     6181 tttctctgca aggctcagca acaccgggtg tgtaggccat tgaaagatcc cttgctgtta
     6241 aacatggttt tgtcgcgatt acttcaattt tacctttacg ttcactgcta tggtattgta
     6301 aagattcttc tttagttaat ttcataaggt actcctttag attaaggaca gattattttt
     6361 taaaaactgg ttaattactt tgtttgatat tttatgcaag tactgttgat tgaatttttt
     6421 taagtattta ggaatttcca gtgaagagaa aatcagccga agctggatta aaaaattaaa
     6481 tgtatcgaag taaatcaaaa tgttcattaa agtagcacta aaaacaatta aataaatcag
     6541 taagaattag taatactaat atttgttacc ccaaaattag ggaaaatttg attgttgtgc
     6601 taaacaattt ttaacttgat ttgaataatc gagcttattt tgtcatagct tcagcttgca
     6661 gatgctccga taattcctta ccgagatatt tatcgataat tgcatgaagc agatatagcg
     6721 ctggagtaac tattatggca attataaact tgtagatata atttacaagg cctattacaa
     6781 gtacaagttt caagtcccat cccgcaccta aataaaatgc tatgaaaaga acaacaaaac
     6841 tatccactaa ctgcgatact aaagtagaac ctgtagcgcg caaccagatt ttagaggaac
     6901 cagtaaaaga tt
//
LOCUS       DASWBY010000094         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_4102, whole genome shotgun sequence.
ACCESSION   DASWBY010000094 DASWBY010000000
VERSION     DASWBY010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4102"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1650)
                     /locus_tag="VF870_01900"
     CDS             complement(4..1650)
                     /locus_tag="VF870_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9250959.1"
                     /translation="MPQRLIFSIIILSLLSSATLLSQTITKENIENAEKLIDLNFTNA
                     ERDSMLGYLEEQKNNYDKIRNVELNNSVMPSLLFNPIPVGFKFPQKLADVKFSDYSNT
                     KMPVNKDELAFYTIGQLAELIRTKQITSTELTKFFIERLKKYDPALHCVITFTEERAL
                     HQAKIADEEIAAEKYRGLLHGIPFGVKDLLATKDYKTTWGAMPYKDQMIDEDATVITK
                     LENAGAVLVAKLTMGALAWGDVWFGGITRNPWDTTKGSSGSSAGSASSVSAGLLPFAI
                     GTETYGSIVSPSTVCGTTGLRPTYGRVSRTGAMALSWSMDKIGPICRSAEDLAIVFNA
                     IEGVDGVDQTLYDIPFSYRPLSNLKGLKIGYLKKDFEKQYDFHNNDSITLTKLKELGA
                     ELIPIELPQFPVNDLAIMLSAEAGAAFDELTRSNKDDLLVRQIKNAWPNSFRASRFIP
                     AVEYINASRVRTLLIQEMQKLMSTIDLFIAATNEGDSNLLTNLTGHPCVVLPNGFSDK
                     ETPTSITFIGRLFDEGRLIVFARLYQDATGYHKKHPDMFK"
     gene            complement(1829..>2227)
                     /locus_tag="VF870_01905"
     CDS             complement(1829..>2227)
                     /locus_tag="VF870_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250960.1"
                     /translation="GISQFAQAQEVGIRFGDAWGGHYAVDGVFGLGQFSRIHADVSFG
                     NDGVGVEALWDLLYKPLEKNLYWYVGVGPSAYLGDPFALAVSGEIGLEYRFDFPMVIG
                     ADWRPSFIIIEETDFEAGWFGLNIRYVFGK"
BASE COUNT          685 a          457 c          381 g          704 t
ORIGIN      
        1 ttttcattta aacatatcgg gatgcttttt atgataacct gttgcatctt gatatagcct
       61 tgcaaataca attaatcttc cttcatcaaa taaccttccg atgaaagtaa tacttgtcgg
      121 tgtttcctta tcagaaaaac cgtttggaag aacaacacat gggtggcccg ttaaatttgt
      181 cagtagattg ctgtcgcctt catttgttgc tgcaataaat aaatctattg tgctcataag
      241 tttttgcatt tcctgtataa gcagagttct aacgcggctt gcatttatat attctactgc
      301 aggaataaat cgcgatgcgc gaaatgagtt tggccaggca tttttaattt gtcttaccag
      361 taaatcatct ttgttgcttc ttgttaattc atcaaaggca gcgcctgctt ctgcagaaag
      421 cattatcgca agatcattta ccggaaactg cggaagctca ataggaatta attccgcacc
      481 aagttcttta agcttagtaa gggtaattga atcgttatta tgaaaatcat attgtttttc
      541 aaaatctttt ttaagatatc cgattttcaa tcctttaaga ttgcttaaag gtctatagct
      601 aaaaggaatg tcgtataagg tttgatcaac accatcaacc ccttcaatag cattaaacac
      661 aattgcaaga tcttctgctg atctgcagat cggaccaatt ttatccatag accagctaag
      721 agccattgca ccggttctgc tcactcttcc gtaagtcggt cttaatcccg ttgtaccgca
      781 aacggtagag ggcgaaacta ttgaaccgta ggtttctgta ccaattgcga atggtaaaag
      841 tcccgcagaa acggaagatg cagatcctgc agaagaccca cttgagcctt ttgttgtatc
      901 ccatggattt cttgttattc cgccaaacca cacatcgccc caggcaagcg ctcccatcgt
      961 taattttgca acaagaacag ctcctgcgtt ttcaagtttt gttattacgg ttgcatcttc
     1021 atctatcatc tggtctttat aaggcattgc gccccaagta gttttgtaat cttttgttgc
     1081 cagaagatct ttaactccaa acggaatacc atgcagcaag ccgcgatatt tttccgctgc
     1141 aatttcttca tcagcaattt tagcctggtg caatgcacgt tcttccgtaa atgttataac
     1201 acagtgaagt gcgggatcat atttttttaa ccgctcaata aaaaattttg tcaattcggt
     1261 tgaagtaatt tgttttgttc ttatcagctc ggcaagctga ccaattgtgt aaaatgcaag
     1321 ttcatcttta ttgacgggca tttttgtatt tgagtaatca ctaaacttta catctgccaa
     1381 tttctgagga aacttaaacc caacagggat tggattaaaa agcaatgatg gcattacgct
     1441 gttgttaagt tcaacatttc taattttgtc ataattattt ttttgctctt caagatatcc
     1501 aagcatggaa tcacgttcag catttgtaaa attaaggtct attaattttt ctgcgttctc
     1561 tatgttttct tttgtaatcg tttgtgataa aagcgttgca gaactgagca gggataatat
     1621 gataattgaa aaaattaatc gctgaggcat aataatctcc ttaatgtata aataaaatta
     1681 tttctaaaag atgaataaaa ctaatttgaa aacgatatac ttacattaaa agaaggaata
     1741 aactcctcac gaataaaatt tataaataca ctaaaacaaa aaaaagacgc acattggtgc
     1801 gtcttctttt tacagaggtc aaaatatttt acttgccaaa aacatatctt atgtttaatc
     1861 caaaccatcc tgcttcaaaa tctgtttctt caataattat aaacgacgga cgccaatctg
     1921 caccaattac cattggaaaa tcaaatctgt attccagccc gatttcaccg cttacagcaa
     1981 gtgcaaaggg atcgccaaga taagcagaag gaccaacacc aacataccag tataaattct
     2041 tttctaatgg cttgtaaaga agatcccata atgcttcaac accaacgccg tcatttccga
     2101 aagaaacatc agcatgtatt ctgctgaact ggccgagtcc aaaaactccg tctacggcat
     2161 agtgtccgcc ccaggcatca ccaaaacgga taccaacttc ctgggcttgt gcgaactgag
     2221 agatacc
//
LOCUS       DASWBY010000095         4471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_154060, whole genome shotgun sequence.
ACCESSION   DASWBY010000095 DASWBY010000000
VERSION     DASWBY010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154060"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VF870_01910"
     CDS             <1..236
                     /locus_tag="VF870_01910"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX9250961.1"
                     /translation="LKSKDIPASAAFPYLTATSENLMKDSDPLSGPVIRDDKKTINAH
                     LKVLNNHPLKNVYISFLEAYDKMKKEKILENNK"
     gene            220..1008
                     /gene="panB"
                     /locus_tag="VF870_01915"
     CDS             220..1008
                     /gene="panB"
                     /locus_tag="VF870_01915"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX9250962.1"
                     /translation="MKTINEFVIKKNKNETISLVTCYDYWSAVIINETDIDAVLVGDS
                     AAMVMHGFDTTVNAEVEMMAYHLASVKRGLIEKFLIVDLPFLAHRKGNKYVMDVVDKF
                     LKLGANAVKIEGAGSNIKTIKHIIDSGVPVMGHIGLTPQSVNSIGGFLLQGKNDITAK
                     KLIDDAKQLEDAGCFSIVLELIPAQLAKDITESIKIPSIGIGAGVYTSGQVLVLQDLL
                     GLTKSFNPKFLRKYLNGFELIKDALNNYDREVKSKVFPNEKESY"
     gene            1008..1772
                     /gene="panC"
                     /locus_tag="VF870_01920"
     CDS             1008..1772
                     /gene="panC"
                     /locus_tag="VF870_01920"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX9250963.1"
                     /translation="MTRVIKRLPEWYKVRRSKIFFHKTVGLVPTMGALHQGHIALIKK
                     SIKQNDITVVTIFVNPTQFNNLEDFKKYPDTIKDDLKILKELKVNFLLNPTYNDIYPD
                     NYEYKIVESNLSKILCGKFRPGHFEGVLTIVMKLLNIVKPDHAYFGEKDFQQLKLIDG
                     MVKAFFIDTKIISHPTVRTKEGLALSSRNLRLSDEDKIKAIEFPKLLSSTLSDDKIKA
                     LLKSKGFKVEYIEQINNRRFGAVYLGNVRLIDNVKI"
     gene            1759..2982
                     /gene="coaBC"
                     /locus_tag="VF870_01925"
     CDS             1759..2982
                     /gene="coaBC"
                     /locus_tag="VF870_01925"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX9250964.1"
                     /translation="MLKSKILFKISGSIAAYKSAYLISKLVQNGYEVQTAATQAALKF
                     IGKASLEGLTGKPVLIDQYEDGKMMNHINLMKWADLIILCPASANTINKMANGISDNL
                     ITSLFLAHNWDKPYLIAPAMNTAMYNHPATKESLRKLKDWGVEILPTDSGYLACGDIG
                     EGKLLEPDIIYDSIVSSLQNNAVNNGKTVLITAGATRESIDGVRFVTNLSTGNTAAAI
                     AEHLSALGYNITYLHGTNTKIPKNKSDKIQFSDFDNLHNVISDLLSRNHYDAVIHLAA
                     VSDYKIDKILLSENEIDSVSGNKLKSDINGLIIKLKPTSKIVDQIKTLSKNKNVILFA
                     FKFSGSSSLDESKKEVDNLFRHSNADYVILNHLQDRTKDGIQSKFYFYNKSDLLKNVT
                     NSTQLADAVEQIIRK"
     gene            2995..3777
                     /locus_tag="VF870_01930"
     CDS             2995..3777
                     /locus_tag="VF870_01930"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX9250965.1"
                     /translation="MLLALDIGNTQIFGGVFDEEKIILQFRKSSRLTSSSDEIGIIFR
                     DVLRENKINPESITEIAMCSVVPDAVYSIRNGCIKYFGIDPFILQAGVKTGLKIKYLN
                     PQEVGSDRIANAISAIQIYPNKNIVVIDFGTATTFDVISKEKEYLGGAILPGIRISME
                     ALESKTAKLPAIEIAFPENAIGKTTKDSIQAGLYYGNIGAMKEIIQRISQEAFDGKKP
                     FVIGTGGFANMFNKTGLFDVIDPELVLRGIYYSKKMNSLKEK"
     gene            3779..4129
                     /gene="panD"
                     /locus_tag="VF870_01935"
     CDS             3779..4129
                     /gene="panD"
                     /locus_tag="VF870_01935"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX9250966.1"
                     /translation="MILTVLKSKIHRATVTGADLNYEGSIAIDKKLIEAANFNKFERV
                     EIYNISNGERFATYVIEGKDGEISLNGAAARLVQKGDLIIIACYAQIDEKEIKSFLPK
                     IVLVDKKNKVINKA"
BASE COUNT         1652 a          634 c          791 g         1394 t
ORIGIN      
        1 atttgaaatc gaaagatatt cccgcatcgg cagcattccc ctacctgacc gctacttcag
       61 aaaatttgat gaaagattca gatccattat caggtcccgt aattagagat gataaaaaaa
      121 caataaacgc tcacttaaaa gtattaaata atcatccgct taaaaatgtt tacatatcat
      181 ttttagaagc ttacgataaa atgaaaaagg aaaagatact tgaaaacaat aaatgaattt
      241 gtaataaaga aaaacaaaaa tgagacaata tcattggtta cctgctacga ttattggtct
      301 gcagtaataa ttaatgaaac tgatattgat gcagtattag ttggcgatag tgcagcaatg
      361 gttatgcatg gatttgatac tactgttaat gcagaagttg aaatgatggc ttatcatctt
      421 gcttctgtaa agagaggact gattgaaaaa tttttaattg ttgatttgcc attccttgcc
      481 cacaggaaag gtaataaata tgttatggat gttgtggata aattcttaaa gcttggggct
      541 aacgctgtta aaatagaagg agcaggttct aatattaaga caattaaaca tattattgat
      601 tccggtgtgc ctgttatggg gcatattggg ctgactcctc aatctgttaa ttcaatcggt
      661 ggatttttac tgcagggtaa aaatgatatc acagctaaaa aattaattga tgatgcaaaa
      721 caattagaag atgcaggatg tttctccata gttcttgaat taattcccgc tcagttggcc
      781 aaagatatta cagagtcaat caagattcct tcaatcggca taggtgcagg ggtttataca
      841 tccgggcaag tacttgtact gcaggattta cttggattaa caaaatcatt taatccgaag
      901 tttcttagaa aatatcttaa tggatttgag cttatcaaag atgcactcaa taactatgat
      961 agagaagtta agagtaaggt gtttcccaat gaaaaggaaa gttattaatg accagagtaa
     1021 taaaaagatt acctgaatgg tataaggtaa gaagaagtaa aatctttttt cataaaacag
     1081 tcggactggt tcccactatg ggagccttgc atcaggggca tatagcgtta attaaaaaat
     1141 ccattaaaca gaatgatata acggtagtaa caatttttgt aaatccaaca caattcaata
     1201 atctggaaga cttcaaaaaa tatccagaca ccattaagga tgatctcaaa atactaaaag
     1261 aactaaaagt aaattttctg cttaatccaa cttacaatga tatttatcct gataattatg
     1321 aatacaaaat agttgagtca aatttaagta aaatactctg cggcaaattt cgcccgggtc
     1381 attttgaagg tgtgctaact attgtaatga aacttttaaa tattgttaaa ccagatcatg
     1441 catattttgg tgagaaggat tttcagcaat taaaattaat tgatggaatg gttaaagctt
     1501 tttttataga tacaaagatt atttcacatc caactgtcag aacaaaagaa ggtcttgcac
     1561 ttagttccag aaacctaaga ctttcggatg aagataaaat aaaagcaatc gagtttccta
     1621 aattactatc atcaactttg tctgatgata aaataaaagc tttactaaaa tctaagggat
     1681 ttaaagtaga gtatattgaa caaattaata acagacgatt tggagctgtt tatcttggga
     1741 atgtgaggtt aatcgataat gttaaaatct aaaatactgt tcaaaatatc cggatcaata
     1801 gccgcatata aaagtgcgta tttaatttca aagctggtac agaatggata tgaggttcaa
     1861 acagcggcaa cacaagcagc tttaaaattt attggaaaag cttctttaga aggtttaact
     1921 ggtaaacccg ttttgattga tcaatatgaa gacggtaaaa tgatgaatca tattaatctt
     1981 atgaaatggg ctgatttaat tattctttgt ccagcaagtg caaatacaat taataagatg
     2041 gcaaatggaa tctcggataa tcttattact tccctgttcc ttgctcataa ctgggataaa
     2101 ccttatttaa ttgctcccgc tatgaacaca gcaatgtaca atcatcctgc taccaaggaa
     2161 tcattaagaa agttaaaaga ttggggcgtt gaaattctac ctactgattc cggttattta
     2221 gcctgcggag atattggcga aggtaaacta ttagaacctg atattattta tgatagcatt
     2281 gtgagttcgc ttcagaataa tgctgttaat aatggcaaaa ctgttttaat tacggctggt
     2341 gccacaaggg aatctattga tggtgtaagg ttcgtaacta acttaagcac gggcaataca
     2401 gctgcagcta ttgcagaaca cctttcagcc ttaggataca atatcactta tctgcatgga
     2461 actaatacta agattccaaa aaataaatcc gataaaattc agtttagtga ttttgataat
     2521 cttcacaatg tgatttctga tttgttaagc cgcaatcact atgatgctgt tattcatcta
     2581 gctgctgtca gtgattacaa gatcgataaa atattattat cggagaatga aattgattca
     2641 gtaagtggaa ataaattaaa atcagatatt aacggcttaa ttataaaact aaaaccaact
     2701 tctaaaatcg ttgatcagat aaaaacatta tcaaaaaata aaaatgttat tttatttgca
     2761 ttcaagtttt cgggaagttc cagtttagat gaatcaaaaa aagaagttga taatttattt
     2821 agacattcca atgctgatta tgtgatatta aatcatttac aggatcggac taaagacgga
     2881 atacaatcaa aattttactt ttataataaa tctgacttat taaaaaatgt tactaactca
     2941 actcagcttg cagatgcagt tgaacaaata atacgaaaat aaacaggagc aaaaatgtta
     3001 cttgctttag atataggtaa tacacaaatc tttggtggtg tttttgatga agaaaaaatt
     3061 attttgcaat ttcgcaaatc atcacgacta acatcatcat ccgatgaaat cggaataatt
     3121 ttcagagacg tattacgaga aaataaaata aatccggaat ccatcactga aatagcaatg
     3181 tgctctgtgg tgcctgatgc ggtttattca attcggaatg gatgcattaa atattttggg
     3241 atcgatccat ttatccttca ggcaggagtt aaaaccggat taaaaataaa atacttaaat
     3301 ccacaggaag ttggcagtga tagaattgca aatgcaattt ctgcaattca gatttatcca
     3361 aataaaaata tagtagttat tgattttgga acagctacaa cttttgatgt catctccaaa
     3421 gaaaaagaat atctgggcgg agcaatctta ccgggaatta gaatttcaat ggaggcatta
     3481 gaaagtaaaa cagcaaaact tcctgctatt gaaattgcat ttcctgaaaa tgctattggc
     3541 aagacaacta aagacagcat ccaggctggt ttatattatg gaaacatcgg cgctatgaag
     3601 gaaattattc aaagaatatc tcaggaagca tttgatggaa aaaagccatt tgtaattggc
     3661 actggtggtt ttgcgaatat gtttaataaa actggtttgt ttgatgtgat tgacccagaa
     3721 cttgttttac ggggtattta ttattcaaaa aaaatgaatt cactaaaaga gaaatgaaat
     3781 gatcttaaca gtattaaaat ctaaaattca tagagcaacc gtaaccggtg ccgatttaaa
     3841 ttatgaagga agcatagcga tcgataaaaa attaatcgaa gctgctaact ttaataaatt
     3901 tgaaagagtg gaaatttata atattagcaa cggtgaaaga tttgcaactt atgttattga
     3961 aggtaaagat ggagaaattt cccttaatgg tgctgccgca aggttagtgc aaaaaggtga
     4021 tttaattatc atcgcttgtt atgcccaaat tgatgaaaag gaaataaaat ccttcttgcc
     4081 aaaaatcgta ttagttgata aaaaaaataa ggtaatcaat aaggcttaag agtctagaaa
     4141 aatatttggt taactgattt ttaaatctaa agtatatttt ttaagcttga tttaaacatg
     4201 atttcaaacc ttctgttgcc tgcagagagg gtgattacat tattgcaatt acttttctta
     4261 ttacgcacca ttaacaaatt aaaattttaa tcaaatttta agaaaaatat tagagtattt
     4321 taagaagcag aaagtaagtt aagattaaaa aaaggagaaa ttatgttaga tctaactcac
     4381 ttacatccaa tgattgttca ttttccaata gcacttttaa tagttggctt tttagctgat
     4441 gcaattggat taatattgaa aaaggatttt t
//
LOCUS       DASWBY010000096         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_266689, whole genome shotgun sequence.
ACCESSION   DASWBY010000096 DASWBY010000000
VERSION     DASWBY010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266689"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VF870_01940"
     CDS             complement(<1..640)
                     /locus_tag="VF870_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX9250967.1"
                     /translation="MKSSLGRGLDALINPNRFNESDEQKTPDYSNIKFDDGKQVDVLA
                     KIAVDFISPNPFQPRLNFDPVTLEELKKSIIANGLIQPITVRRIAGNQYQLVSGERRL
                     RAYTDIGYKEIPAYIIKVDSDEIMLALALIENIQRETLNPIEVSKAYKRLMDECHLTQ
                     EQIADKVGKDRTTIANSIRLLKLPDQIQKCLVDESISAGHARALINLPGEDLQ"
     gene            complement(654..1427)
                     /locus_tag="VF870_01945"
     CDS             complement(654..1427)
                     /locus_tag="VF870_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9250968.1"
                     /translation="MAKIISIANQKGGVGKTTTTINLSSLLAAAEKRTLLVDIDPQSN
                     SSSGLSVTNHTPSVYEVLVGTRKIQDVIINTFMPFLDMLPSNINLVGAEIEMVDIENR
                     ETLLRSALAEISDKYDYILIDCPPSLGLLTLNSLTASNSVLIPVQCEYFALEGLGQLL
                     NTINIVKKHYNKDLAIEGVLLTMFDTRLRLSHQVAEEVRKYFGDKVYNTVINRNVRIS
                     EAPSYGKPIILYDAISSGAQNYMALASELLEKNSGILNN"
     gene            complement(1495..2172)
                     /locus_tag="VF870_01950"
     CDS             complement(1495..2172)
                     /locus_tag="VF870_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX9250969.1"
                     /translation="MKLRYFGHSAFQITTKSGIKILIDPFLDDNPVSPVKSKDVNAEF
                     IILTHGHGDHVGDGLKIGKRCNSLFICVNELAEWIKSKGLNAHNMHIGGAHNFDFGRV
                     KFTIAHHGSLSPDNQYTGEASGVLVTIEGKTIYHTGDTGLFYDMKLIGEMNPIDYMLL
                     PIGDNYTMGIKDAVKAVELANPKTAIPIHYNTFPVINSDPNEFKKLVEANGTKCKVLN
                     FGEEIEL"
BASE COUNT          705 a          415 c          334 g          839 t
ORIGIN      
        1 gctgtaaatc ttctccgggt aaattaatta acgctctggc gtgccctgct gaaatacttt
       61 catcaactaa acatttttga atttgatccg gtagtttaag taatcgtatt gagtttgcga
      121 ttgtagttct atctttccca actttatcag caatttgctc ctgagttaag tggcattcat
      181 ccatcaatct tttgtaagct ttacttactt caatcggatt taatgtttct ctttgaatat
      241 tctcaatcaa agccagagcc agcattattt cgtctgaatc aactttaatt atgtaagccg
      301 gaatttcttt gtagccaata tctgtataag cacgcaatcg tctttcacct gaaactaact
      361 ggtactgatt acccgcaatt cttctaacgg ttattggttg aattaaacca ttagcaatta
      421 tcgatttctt taattcttca agtgtaacgg gatcaaaatt taatcttggt tgaaatggat
      481 ttggcgagat aaagtcaaca gcaatcttag ctaacacatc aacttgtttg ccatcatcaa
      541 acttaatatt ggaataatct ggtgtctttt gttcatcact ttcattaaat ctgttgggat
      601 tgattaatgc gtccaatcct cttcctaaac ttgatttcat tttattcata tctttaatta
      661 tttaaaatac ctgaattttt ttccagcaat tctgacgcaa gtgccatata attttgtgct
      721 ccgcttgaaa tagcatcata aagaattatt ggtttgccgt aacttggtgc ttctgaaatt
      781 ctaacgtttc tgttgatcac agtattataa actttatccc caaaatactt tcttacttct
      841 tcagcaacct gatgtgataa tcttaatctc gtatcaaaca ttgtaagcag tacgccttca
      901 atcgccaaat ccttattata atgtttttta acgatattga ttgtattaag taattgccct
      961 aatccttcta atgcaaagta ctcgcactga actggtataa gaacagaatt tgatgctgta
     1021 agtgaattta gagtgagtaa acccaaagac ggcggacaat ctattaatat ataatcgtac
     1081 ttgtcgctta tttctgccaa agcacttctt aaaagcgtct cacgattttc aatatcaacc
     1141 atttctattt ctgctcctac aagatttatg tttgatggga gcatatcaag aaaaggcata
     1201 aaagtattta ttataacgtc ctgtattttt cttgttccaa ctaaaacttc atagaccgaa
     1261 ggagtatgat tcgttacact caatcctgat gaagaatttg attgcggatc aatatctact
     1321 aataaagtac gtttttcggc agcagccagc agtgaagata aattaatggt agtagttgtt
     1381 tttcccactc cacccttctg atttgctata gaaattattt ttgccatcta attagaatta
     1441 ttaaaaaatt gagttctaag ataatcgatt gtaagctata tataaacaat aaaattataa
     1501 ttctatctct tcaccaaaat ttaaaacttt acactttgtt ccgttggctt caacaagttt
     1561 tttaaattca tttggatcag aattgattac aggaaaagta ttataatgta tcggaattgc
     1621 agttttagga ttcgccaact caacagcttt tacggcatct tttataccca ttgtataatt
     1681 atcaccaatt ggtaaaagca tataatcgat aggattcatc tcaccaatta atttcatatc
     1741 ataaaataat cctgtatcgc cggtatgata aattgtttta ccttcaatag taactaaaac
     1801 tccagatgct tctccagtat attgattgtc cggtgataaa gatccatgat gtgcgatagt
     1861 aaattttact cttccaaaat caaaattatg tgctccgcca atatgcatat tatgtgcgtt
     1921 aagtcctttt gatttaatcc attcagccag ttcattaaca caaataaata gcgagttgca
     1981 gcgtttacct atttttagtc catctccaac gtgatcacca tgaccgtgag taagaataat
     2041 aaattctgcg ttaacatctt ttgattttac ggggctgaca ggattatcat ccaaaaatgg
     2101 atctattagt atttttatgc cgcttttagt tgttatttga aaagctgaat gcccaaaata
     2161 tctaagtttc atggcgtttc tcctctgtta agatagttgt ttgataaaat ctttaagaac
     2221 attacttaag gtaaatttca ttaactctaa tttaaatttt tcttctaata acaatttctt
     2281 ttcaattgct tta
//
LOCUS       DASWBY010000097         7897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_41264, whole genome shotgun sequence.
ACCESSION   DASWBY010000097 DASWBY010000000
VERSION     DASWBY010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41264"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VF870_01955"
     CDS             <1..887
                     /locus_tag="VF870_01955"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX9250970.1"
                     /translation="LQDKDSKQEAVGENLFIVRDYNKDISLSDILMISKYDSSQGRNE
                     IIPNISRNIPTSKDRVKFYYEVYSKDSTDVNRTFEYTVFNSEKNVIHKEVEDRNIKPG
                     KNKVLYSLAEFPFELGIYSLTVSLLDENSKVIETVSKAFYSHWKGLPGVITDIDKAIS
                     QTVYIATPDEMNYMEEGETIQEKTKRFLEFWKKKDPSPNNDENEIFDEYFRRISYSND
                     NFSNYIEGWRSDRGMVYTILGAPNNVDRHPFEYDSKPYEVWEYYDLNRSFVFLDQTGF
                     GDYRLITPLTGDMYRYRY"
     gene            909..1880
                     /locus_tag="VF870_01960"
     CDS             909..1880
                     /locus_tag="VF870_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9250971.1"
                     /translation="MILIITLNPLLERRFNYKQVTSGTVNRNAITKNSAGGKGINVSR
                     QLKKFGIKSYNYFFSGGTNGKIYRDLLKSEELDFSFISTKSETRHAAVITSEKDKAVS
                     SYFSEDPKISQTEVEEFKSKMDKMIQNCEIVIFSGSSPSRETDSIFPFGIELANKYDK
                     VSICDTYGKHLQNCIDTEPTIIHNNLFEIKNSIDVKLNAEKSIVDFLNQLYNKNIKRA
                     YLTNGSNNFYASNFDYIYKIKPLEIQEIDATGSGDTFVASIVKGWINSDLFEDSLKFA
                     TAAAGLNAAAFEVADVNPEDAIKFKDKVEVLPVGKKTKTIDDSPREI"
     gene            1858..3624
                     /locus_tag="VF870_01965"
     CDS             1858..3624
                     /locus_tag="VF870_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9250972.1"
                     /translation="MIRRAKFSLVIVLFISSFLSAQKLNSFEVNGNINFEDSDYQKWA
                     ELSLNQPIYNGIIDSVKSKIARNILLQGYYFFNFKNTELIVSNDSTKYDIVIEINEGD
                     PVIINNIYISGIDTSADISIIKQFDYLKGQIFNKQEIESNIEQLLTKFENAGYPFAKI
                     QINSVSVYRDSIAEENFADVYLNIISGEESKIDRVEIRGNTSTKDYVIIRELRIDSAE
                     PYMQNKIEEFPKRLNRLRFFEPVPLPQFYINSKKEGVLLVEVKEKNTNNFDGIIGYIP
                     PGKNENSGYITGLVNISLRNLFGTGRAASIRWNKYNRNSQELDLRYLEPWFLNFPLNI
                     NLGLYQRVQDTTYVQRKITGSLEYLATEDISASVLIASETVVPTIRTVPVFTVYNSSY
                     ISTGANLKIDTRDDPYSPTEGVLFINTYSFSIKTINGPEEYFTPTLNTSVNLQRFESS
                     FYFFYELFLRQIIAIGVNGRELRGSTFENSDLYRLGGTNSLRGYREEQFLGSRIFWSN
                     LEYRALLTRRSYGFVFFDTGYYLRPEEPDRNIPKSEEFLYGFGLGLNLETGLGVLRVS
                     YALGKGDTFSDGKIHFGVLNEF"
     gene            3694..4539
                     /locus_tag="VF870_01970"
     CDS             3694..4539
                     /locus_tag="VF870_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HEX9250973.1"
                     /translation="MNLSKQIFAYLKITRPLNVALTFFVVVVAILISKQDDVNWLIIF
                     LASLSAAFTTASGNIINDIFDIETDRISHPNRALVLGVLTKKSAAIEYLLLNLFAIII
                     AGYLSIKLMLIVLISIIILFLYSYSFKKKPLIGNVTVAFLTGLAFIYGGIAAENPVAA
                     IVPAVFAFLINLIREVVKDMQDVEGDIKAGVSTFPIKFGFQNSKIIIVISTIILIFCT
                     LYPFITQLYKIEYFLIVMLFVNPLLILCIKYLFDVNKENKLSTVSNILKINMVLGLIA
                     IYLGK"
     gene            4536..5162
                     /locus_tag="VF870_01975"
     CDS             4536..5162
                     /locus_tag="VF870_01975"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX9250974.1"
                     /translation="MKNFDKKYLSEKIKIIAGTDEAGRGPLAGPVVAAAVILPNDFYD
                     ERIDDSKKLSANMREELFNVIRKNALSYAYTIISQNKIDEINILKASLLAMKRSVEKL
                     KLKPDLVLVDGNKTFIYDGEVIPIIKGDSKSLSIASASIIAKVVRDRTMMKLAREYPI
                     YGWETNKGYPTKVHIEAVLKFGACKIHRKTFLTKIFERSDQTELSFID"
     gene            5171..5536
                     /locus_tag="VF870_01980"
     CDS             5171..5536
                     /locus_tag="VF870_01980"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX9250975.1"
                     /translation="MAEKNKRDIGKEGEDIAAKYLTEKGFEIIARNYHYSHGEIDIIA
                     NDKQQLVFIEVKTRLNLEYGEPEYAINPKKVKQIKKMAELYLFDKEIEEAECRFDVVA
                     IILCDSSNPIITHYENAFY"
     gene            5648..6454
                     /locus_tag="VF870_01985"
     CDS             5648..6454
                     /locus_tag="VF870_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9250976.1"
                     /translation="MNNSNGSNIKISHFGKQVSEFFIMIAGLWQFQWQFFKQVFIPPY
                     EIEQIRKHMDELGIKTLPIVSVTGFIIGLVITMQMHPVMVRFGAEAFLPGSVGISIVR
                     ELGPVITALIFAGRVSSGIGAELGSMRVTEQIDAMEVSGVNPFKYLVVTRIFACTFIL
                     PILTIYVIFIAFVGSYIAVVLVQNMSYVYYLDSVIGGVEFGDAIPGILKTFVFGFIVG
                     IVGSYKGYTATNGTEGVGKAATTAVVVSSLMILIFDMVLVKVTLWLWPTF"
     gene            6465..7223
                     /locus_tag="VF870_01990"
     CDS             6465..7223
                     /locus_tag="VF870_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9250977.1"
                     /translation="MKTVVDIVNVSKAFGSHVVLDNVSLTVEEGENLVVFGQSGTGKS
                     VLLKCIVRLLEPDSGQIYIDGNNVLQLNPKELNNVRKNLGFLFQGAALYDSMSVRENL
                     EFPLIRHFNFTPREREEKIKSVLEKVSLEEAIDKMPSELSGGMKKRIGLARSIISEPK
                     LMLYDEPTTGLDPITSKEISSLILELQKSLQMTSIIVTHDLLCAEIIADRAIVLNEGI
                     VQHSGTITELTTSSDPFLRNFFSHEIVEDQNRRN"
     gene            7223..>7897
                     /locus_tag="VF870_01995"
     CDS             7223..>7897
                     /locus_tag="VF870_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9250978.1"
                     /translation="MFKNLANAKLGLFIFLGSTLLVVLIFLLGNKDQLFTSTFTVKTY
                     FQNTGGLRNGASVRFGGIDVGAVKDVKIVNDGTGRIEVSMRIKDEIKPFLKKDSRASI
                     ETEGLVGNKVIVISMGSDKSEGISDGGVILSTEPLSFANIIEETQGIMAYTKDMTKDL
                     AEIVNKVNNGEGTIGKILNDDQLYNAATNLTKSADKSMVSLTDDMKGVIDLFDELGKG
                     VQDVVSN"
BASE COUNT         2730 a         1111 c         1473 g         2583 t
ORIGIN      
        1 tgcttcagga taaagattcc aaacaggaag cagttggaga aaatctattt atagtcagag
       61 attataataa agacatttca ttaagcgata tcttaatgat ttcaaaatat gatagttcac
      121 aaggcaggaa tgaaatcatt ccaaacatca gcagaaatat tccgacatca aaagaccggg
      181 taaaatttta ttatgaagtc tattcaaaag acagtactga tgtaaataga acttttgagt
      241 acacagtttt taattctgaa aagaatgtaa tacataaaga agttgaagat agaaatatta
      301 agccgggtaa aaataaagtt ttatactcgc tggcggaatt tccttttgag ctcggtattt
      361 attcattaac agtttctctg ctggatgaaa attctaaagt aattgaaact gttagcaagg
      421 cattttattc tcattggaaa ggcttacccg gtgtaattac tgatattgat aaagcaattt
      481 cacaaacggt ttacatagct actcccgatg aaatgaatta tatggaagag ggtgaaacca
      541 tacaggaaaa aacaaaaaga tttttagagt tttggaagaa aaaagatcct tcaccaaaca
      601 atgatgaaaa tgaaatattc gatgagtact ttagaaggat atcatattca aatgataatt
      661 tttcaaacta catcgaaggc tggagatcag atcgcggaat ggtttatacg attcttggcg
      721 ctcccaataa tgtggatagg catccgtttg agtatgattc taaaccatac gaagtttggg
      781 agtattatga tttgaacaga agttttgtat ttcttgatca gactggattt ggtgattaca
      841 gattaattac tccgttaaca ggtgatatgt atcgttacag atattaattt ttttcgaact
      901 tttagattat gatcttaatc atcacgctaa atccgctgtt agaacgaaga tttaattata
      961 aacaggttac gtcaggaact gttaatagaa atgcaattac aaaaaattct gctggtggaa
     1021 aaggaataaa tgtaagtcgc cagttaaaaa agtttggcat aaaatcttac aattattttt
     1081 tttcaggggg aacaaatgga aaaatttatc gtgatttatt aaaaagtgaa gaacttgact
     1141 tttcttttat ctcaacaaaa tcagaaacaa gacacgctgc cgtaattact tcagaaaaag
     1201 ataaagcagt ttcatcatac ttttctgaag acccaaaaat ttctcaaact gaagttgaag
     1261 aattcaaatc taaaatggat aagatgattc agaattgtga gatagttatt ttctcgggaa
     1321 gttctccatc tcgcgaaaca gattcaatat ttccttttgg tattgagctg gcaaataaat
     1381 atgataaagt ttcaatatgt gatacttatg gtaagcattt gcagaattgt attgatacag
     1441 aacccacaat aattcataat aatctcttcg aaatcaaaaa ctcaattgat gtaaagctaa
     1501 atgctgaaaa atcaatcgta gattttttaa atcagctcta taataaaaac ataaaaagag
     1561 cctatttaac aaatgggagt aataattttt acgcttctaa tttcgattac atttataaaa
     1621 ttaaaccatt ggaaattcag gagattgatg caactggaag cggcgatact tttgtagcat
     1681 ccattgttaa aggttggatt aattctgatt tgtttgaaga ttcattaaaa tttgcaacag
     1741 cagctgcagg attgaatgcg gcagctttcg aggttgctga tgttaaccct gaagatgcaa
     1801 ttaaatttaa agataaagta gaagttttac cggttggaaa aaaaacaaag acaatcgatg
     1861 attcgccgcg cgaaatttag cctagtaata gttcttttta tctcttcttt tttatcagca
     1921 caaaagctta attcgtttga agtgaacggt aatattaatt ttgaggattc agattatcaa
     1981 aagtgggctg agcttagttt aaaccagcct atttacaatg gaataattga ttcagtaaaa
     2041 tctaagattg caagaaatat tttattgcag gggtattact ttttcaattt taaaaacaca
     2101 gaattgattg tttccaatga ttcaacaaag tatgatatcg tcatagaaat aaatgaaggc
     2161 gatcctgtta ttattaataa tatctatata agtggaattg acacctctgc agatataagt
     2221 attattaaac aatttgatta tttaaaagga cagattttca ataaacagga aattgaatcc
     2281 aacatagagc aattgttgac caagttcgaa aacgccggtt atccttttgc aaaaattcag
     2341 ataaattcag taagtgttta tagagattct attgctgaag aaaattttgc agatgtctat
     2401 cttaatatta ttagcggtga agaaagtaaa attgatagag ttgaaatccg cggtaatact
     2461 tcaacaaaag attacgttat tattcgtgaa cttcgtattg attccgctga accatatatg
     2521 caaaataaga ttgaagaatt tcctaaaaga ttaaatcgcc ttcgtttttt tgagccggtt
     2581 ccattaccgc agttttatat caactctaaa aaggaaggtg tactgcttgt tgaagtgaaa
     2641 gaaaaaaaca caaacaactt cgatggaatt attggatata tcccgccggg taaaaatgaa
     2701 aattcaggat atataaccgg gcttgtaaat atttccctgc gaaatttatt cggcaccgga
     2761 agagccgctt ccatcagatg gaataaatac aacaggaatt cgcaggagtt ggatttaaga
     2821 tatcttgaac cttggttttt aaattttcct ttaaatatta atcttggact gtatcaaagg
     2881 gtgcaggata cgacttacgt gcaaagaaaa attacaggct cgttagaata tcttgctact
     2941 gaagatatct cagcatccgt tttaatagca tcagaaactg tggtgcctac aataagaaca
     3001 gttccggtgt ttacagttta taattcatca tacatttcaa caggagcaaa tttaaaaatt
     3061 gatacgcgtg atgatccata ttctccgaca gaaggagtat tatttataaa tacatattca
     3121 tttagtataa agactattaa tggtccggaa gaatatttca ctccaactct gaatacatca
     3181 gtaaatcttc aaagattcga atcaagtttc tacttttttt acgagttatt tttacgacag
     3241 attattgcaa ttggtgtaaa cggaagagag ctgaggggat caacttttga aaacagtgat
     3301 ctttacagac ttggtggaac aaactcatta cgcgggtata gagaagagca gtttttagga
     3361 tcaagaatat tttggtctaa tctggaatat cgtgcattgt taacaagaag atcctacggc
     3421 tttgtttttt ttgataccgg gtattatttg cgtccggaag aacctgatag aaatattccc
     3481 aaatcagaag aatttcttta tggttttggt ctgggattaa acctggaaac cggacttgga
     3541 gttttaagag taagctatgc gcttggtaaa ggcgatactt tcagcgatgg taagatccat
     3601 tttggtgttt taaatgagtt ctaatataaa ttttcaaaag tttcactttt agtggtgctt
     3661 caattatttt ggagcagcat tttttatttc aagatgaacc tttcaaaaca aatttttgca
     3721 tatttaaaaa taacccgacc attaaatgtt gcgttaacat tttttgttgt tgtagttgct
     3781 atcctgattt caaaacagga tgatgtaaat tggttaataa tttttttagc atccctttcg
     3841 gctgctttta caactgcatc tggaaatata ataaacgata tttttgatat agaaactgat
     3901 cggatttccc atccaaacag agcattagtt ttgggtgtat taacaaaaaa gtctgcagca
     3961 attgaatatc tactacttaa tctttttgca attattattg ctggttattt atctatcaag
     4021 cttatgttga ttgtattaat ctcgataatc atattgtttt tatattccta ttcattcaag
     4081 aagaaaccac tgattggtaa tgtaaccgtt gcctttttaa caggactggc attcatttat
     4141 ggaggaattg ctgctgaaaa tccggttgcc gcaatcgttc ctgctgtgtt tgcatttcta
     4201 ataaatctaa ttcgtgaagt tgtgaaggat atgcaggatg ttgaaggtga tataaaagca
     4261 ggtgtgtcaa ctttcccaat taaattcggt tttcaaaatt caaaaattat aattgtgata
     4321 agtaccatca tccttatttt ttgtacccta taccccttca tcactcaact ttacaaaatt
     4381 gaatactttt taattgtgat gttatttgta aatcctttgt taatcctttg tataaaatat
     4441 ttattcgatg ttaacaagga aaacaagcta tccactgtta gtaatattct aaagataaat
     4501 atggtactgg gtttaatagc aatttatctt ggtaaatgaa aaattttgat aaaaaatatt
     4561 tatctgaaaa gataaaaata attgcaggaa cagatgaagc aggcagaggt ccgcttgcag
     4621 gtcctgttgt ggcagcagca gttattttac ccaacgattt ttatgatgaa agaattgatg
     4681 attcaaaaaa actttcggca aatatgcggg aagaactttt taatgtgatt agaaaaaatg
     4741 ctttgagcta tgcttacaca ataatatcac aaaataaaat tgatgaaata aacattctta
     4801 aagcatcatt actagcgatg aaaagatcgg tggaaaaatt aaaattaaag ccggatttgg
     4861 ttcttgttga tggcaataaa acttttattt atgatggaga agtgatccct attattaaag
     4921 gagattcaaa atcgttatca atcgcttcgg catcaattat agcaaaagtt gtacgggata
     4981 gaactatgat gaaacttgca agagagtacc caatttatgg ctgggaaaca aataaagggt
     5041 atccgacaaa agtgcatatt gaagcggtcc taaaatttgg tgcctgcaaa attcatagaa
     5101 aaacattttt aacgaaaata tttgagcgct ccgatcaaac agaattatct tttattgatt
     5161 gaggttttct atggctgaaa aaaacaaaag agatattggt aaagaaggtg aagatattgc
     5221 tgccaaatat ctaactgaaa agggatttga aattatcgcg agaaattatc attatagcca
     5281 cggtgagatt gatataatag ctaacgataa acagcaattg gtttttattg aagttaaaac
     5341 gcggctgaac cttgaatacg gtgaacctga atacgcaatc aatcctaaaa aagttaagca
     5401 gataaaaaaa atggctgaat tatacttgtt tgataaggaa attgaagaag cagaatgcag
     5461 gtttgatgtt gtagcaataa tcctttgtga tagctccaat ccaattatta ctcattatga
     5521 aaatgcgttt tattaaaacc ggtgtgaatt gtttcacaat ttggaaaaat cgctaaaaat
     5581 tattcctaaa attttcttag tttgaaatca acaaataaaa aacttatcta aaaaagtgac
     5641 atcatcaatg aataattcta acggttcaaa tattaagatc agtcattttg gtaagcaggt
     5701 atcagaattt ttcataatga tcgcgggtct ctggcaattt caatggcaat tctttaaaca
     5761 ggtttttata cctccatatg aaattgaaca aattcgcaag catatggatg aacttggaat
     5821 taaaacactt ccaattgtta gtgtaacagg atttattatt ggattagtaa tcacgatgca
     5881 aatgcatccc gttatggtaa gatttggtgc tgaagctttt ttgcccggtt ctgtaggaat
     5941 ttcgattgta agagagctgg gacctgtaat tactgcatta atatttgctg gaagagttag
     6001 ctcaggtatt ggtgcagagc ttggctctat gcgtgttacg gagcagattg atgctatgga
     6061 agtctcaggc gtaaatccat tcaaatatct tgttgttacc agaatttttg cctgcacatt
     6121 tattcttcct atactaacca tttatgttat ctttatagct tttgttggat cttatatagc
     6181 tgtagtactt gtacaaaata tgtcatatgt ctactatctg gattctgtaa taggaggagt
     6241 tgaatttggt gatgcaattc ctggtatttt aaaaacattt gtgtttggat ttattgtagg
     6301 tatagttgga tcttataagg gatacactgc aactaacggt actgaaggtg tcggtaaagc
     6361 cgcaactacc gctgtagttg tttcttcgtt aatgatttta atttttgata tggtgcttgt
     6421 aaaagtaact ttatggttat ggcctacatt ttaaaaagta taaaatgaaa acagttgttg
     6481 atatagtaaa tgtttctaaa gcgtttggtt cacacgtcgt gcttgataat gtttcgctta
     6541 cggttgagga aggcgagaac ctggttgtat ttggccaaag cggaacaggc aagagtgttt
     6601 tattaaaatg tattgtaaga ttattagaac cggattccgg tcaaatttat atagatggaa
     6661 ataatgtgct ccaattaaat cccaaagaat taaataatgt tcgtaaaaac ttgggatttt
     6721 tatttcaagg tgcggccttg tatgattcta tgagtgtaag ggaaaacctg gagtttccat
     6781 taataagaca ttttaatttt acgccaaggg aaagagaaga aaaaattaag tcggttcttg
     6841 aaaaagtttc tctggaagaa gcaattgata aaatgccttc tgaactttcc ggtggaatga
     6901 aaaagagaat cgggttagca agatctatta tttctgaacc aaagcttatg ctctacgatg
     6961 aaccaactac gggacttgat ccaataactt caaaagaaat cagttctttg atactagaat
     7021 tgcagaaatc attacaaatg acttctataa ttgttactca cgatttatta tgtgcagaaa
     7081 ttattgcaga tcgtgcaatt gttttaaatg aaggtattgt tcagcattca ggtacaataa
     7141 cagagcttac aacctcaagt gacccttttt tgcgaaattt ctttagtcat gaaatagttg
     7201 aagatcagaa caggagaaat taatgtttaa aaatctagct aatgccaaac ttggattatt
     7261 catctttctt ggaagcacat tacttgtggt tcttattttt cttttaggaa ataaagacca
     7321 gctttttact tctaccttta cagttaaaac ttatttccag aacaccggag gtttgcgtaa
     7381 cggagcttct gtaagatttg gcggtattga tgttggtgcg gttaaagatg ttaagattgt
     7441 taatgatggt accggcagaa ttgaagtatc gatgcgtata aaagatgaaa taaaaccgtt
     7501 tctaaaaaaa gactcacgtg ctagtattga aactgaagga ctagttggta ataaagttat
     7561 tgttatatca atgggttctg ataaatcaga aggaatttcg gatggaggag taatactatc
     7621 aactgaacca ttaagctttg cgaatattat cgaggaaaca cagggtataa tggcttatac
     7681 aaaagatatg actaaagatc ttgcagaaat agtgaacaaa gtaaataatg gtgaaggaac
     7741 gattggaaaa attttaaatg atgatcagtt atacaacgct gctacaaatc ttacaaagtc
     7801 agcagataag agtatggtct ctttaaccga tgatatgaaa ggtgtaattg atctttttga
     7861 tgaattagga aagggtgttc aggatgtagt tagcaat
//
LOCUS       DASWBY010000098         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_4161, whole genome shotgun sequence.
ACCESSION   DASWBY010000098 DASWBY010000000
VERSION     DASWBY010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4161"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..729)
                     /locus_tag="VF870_02000"
     CDS             complement(163..729)
                     /locus_tag="VF870_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX9250979.1"
                     /translation="MELILSIFIGVGLAAAVGFRIFIPFLITSIAAYTGNLELSTYFS
                     WIGSLPALILFSVATVVEIFSYYIPWVDNFLDAINHPLAIFAGIILSGAVVTDFPPLI
                     KWSLALIAGGGIAGTIHAATGLIRLKSSALTGGAGNPVVSTAEAGSSVALSVIAIFIP
                     AVAVLIVGIMIVYFSFMIKKKFFSPPTE"
     gene            complement(770..1798)
                     /gene="cydB"
                     /locus_tag="VF870_02005"
     CDS             complement(770..1798)
                     /gene="cydB"
                     /locus_tag="VF870_02005"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX9250980.1"
                     /translation="MEFTFDLNTIWFFLIGILLVGYAILDGFDLGVGALHLLVKDDLE
                     RRIMLNSIGPVWDGNEVWLVTGGGALFAAFPHVYATVFSGFYSALMMLLFMLIFRAVA
                     IEFRSKRPMKWWRQMWDVAFSVASIFIAFLAGVSIANLITGVPLGPDKEFIGTFWGLI
                     NPYTILVGITTVALFMMHGAIYGVMKTEGELHKKLRGWVNNTIIFFIICYITTTMATL
                     IYYPHMIQHFKEFPALFVLAILNMLAIANIPREISKGRDFFAFLSSCASIAALLSLFA
                     FGLFPNFVLASNNSEFSLNIYNSASSQKTLNIMLIIAMIGVPFVLAYTISIYWIFRGK
                     VKLNNMSY"
BASE COUNT          691 a          359 c          301 g          504 t
ORIGIN      
        1 acaagctgga tagagttaaa aatatctgaa ggtaaaaaca gacaaattag aaggatgaca
       61 gcagcagttg gatatccgac attaagatta gtacgtttct caataggcga ttggacgata
      121 gagaatctaa accctgggaa tcatattgtt atttaaagat tactattcag ttggcggtga
      181 aaaaaacttt ttcttaatca taaaagaaaa gtatacaatc ataatcccaa caattaaaac
      241 cgcaactgca ggaatgaata tagcaattac agaaagcgcc actgagctgc ctgcttctgc
      301 agttgaaact actggatttc ctgccccgcc ggtgagcgca gatgatttca atctaatcaa
      361 tcctgtagct gcatgaattg ttcctgctat tccgcctcca gcaattaaag ccaatgacca
      421 tttaatgagc ggaggaaaat cagttactac agcaccactt aaaattatcc ccgcaaatat
      481 tgcaagcgga tgattgattg catctagaaa attatccacc catggaatgt agtagctaaa
      541 aatctcaaca acggttgcta cagaaaaaag aattagtgct ggtaatgagc caatccatga
      601 aaaataagtt gataactcca ggttacctgt gtatgcagca attgatgtaa taagaaaggg
      661 aatgaaaatt ctaaaaccaa cggcggcagc aagtccaacc ccaataaaaa tactaagtat
      721 taattccatt aatgctttct cattttatga ttcttaattt ttaattgcct caataactca
      781 tattattcag cttaactttt ccccggaata tccaatagat acttatagtg tatgcaagaa
      841 caaaaggaac accaatcata gcaattataa gcataatatt taaagttttt tgagaagatg
      901 cggaattata aatattcaaa ctaaattctg agttattaga agcaagcaca aaattgggaa
      961 ataagccaaa tgcaaacaac gaaagtaatg cagctattga tgcacaggaa gaaagaaatg
     1021 caaaaaagtc gcgtccttta gaaatctctc ttggaatatt agcaattgct aacatattta
     1081 aaatcgcaag tacgaacaac gcaggaaatt ctttaaagtg ctgaatcatg tgagggtaat
     1141 aaattagtgt tgccatagta gtagtgatgt aacaaataat aaaaaatata attgtattat
     1201 ttacccatcc tcttaatttt ttatgcagct cgccttcagt tttcataaca ccataaattg
     1261 caccgtgcat cataaacaaa gccacggttg ttatcccgac taaaattgtg tatggattta
     1321 tcaaacccca gaatgttcca ataaattctt tgtctggtcc aagaggaaca cctgtaatta
     1381 aatttgcaat cgaaactcct gcaagaaaag caataaaaat acttgcaacg ctaaaagcaa
     1441 catcccacat ttgtctccac catttcatag ggcgtttact tctaaactca attgcaacag
     1501 cacggaaaat aagcatgaat aacaacatca ttaatgcaga gtaaaaacca gaaaacacag
     1561 tcgcgtaaac gtgtggaaaa gctgcaaaca aggcaccgcc tccggttacc agccagactt
     1621 cattcccatc ccaaacagga ccgattgaat taagcattat tcttcgttca agatcatctt
     1681 ttacaagaag atgtaatgct ccaactccaa gatcaaatcc atcaaggatt gcatatccaa
     1741 ccaataaaat tccaattaag aaaaaccata ttgtatttag atcaaaagta aattccatat
     1801 tactccttaa actcttttat gcctttggat gtccaatttc agaaggtatc gaatc
//
LOCUS       DASWBY010000099         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_41350, whole genome shotgun sequence.
ACCESSION   DASWBY010000099 DASWBY010000000
VERSION     DASWBY010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41350"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2739
                     /locus_tag="VF870_02010"
     CDS             <1..>2739
                     /locus_tag="VF870_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9250981.1"
                     /translation="FDKTLLKFVEEFPPLPVYQNKVKAIEYLKGKTENKDNREILLEE
                     LIILNLENINPATHQLKELYTDEKLVKETKYKEIIEHTELFFENEPKIGGFNLISFLR
                     KPIIQSPYNIEEQLDFIRANWSSFIDETLLSKILSGKDLIHEDYKLFVQHGGGEKGTP
                     PVPAYEFDYKYFESLKLKLKEGQKLSPEEYEYYQLEVNRFTVDIDWMPRVVMIAKNAF
                     VWLHQLSKKYGREIKTLDQIPDIELDTLARWNFTALWLIGIWERSSASRKIKQMMGNP
                     EAASSAYSLYDYVIANELGGESAFENLKARAWQRGIRLSSDMVPNHTGIYSKWVTEKP
                     EYFIQNKVPPYPNYKFTGPNLSDDDRVEVRVEDQYYSRTDAAVVFERLDRLTGDRTYI
                     YHGNDGTNMPWNDTAQLDLMKAEVRESLIQTIMHVARKTPIIRFDAAMTLAKKHYQRL
                     WFPIPGSGGAIPSRADYAMTRSQFDAIMPEEFWREVVDRINAEMPDTLLLAEAFWLME
                     GYFVRTLGMHRVYNSAFMHMFMKEENEKYKLLIKNTLAFNPEILKRYVNFMSNPDEET
                     AINQFGKGDKYFGVATMLVTLPGLPMFAHGQIEGLSEKYGMEYKRAYYDEFTDDNLVA
                     RHEKEIFPLTKKRYLFSQVNNFELYDFMDPNGICNENVFAFTNKDGNEVSLVVYNNSY
                     SEAIGTINLSCERAYGEEGRTSTTTIDKALGINYGYGFYYIFKDYIQQLEFIVSGSEI
                     SSTGFFFHLMGYQRKVLLDFREVYDTDGRYTVLYQQLNGRGVNSIERALSEMNLAPFH
                     NSLVHFFTSINFSRLLLSLDFYNKDEIEIPEVDEELTRIIKSLNKIKSIETSLSELTA
                     QIQSDISDISRFNKLFLEISSTKRPAKWIGDVKNILYPQNDIERKRLLSFLLLNKII"
BASE COUNT          958 a          440 c          516 g          825 t
ORIGIN      
        1 tttgataaga ctttactgaa atttgttgaa gagtttccgc ctctccctgt ttatcaaaat
       61 aaagtaaaag ctattgaata ccttaaaggg aaaactgaaa ataaagacaa ccgcgagata
      121 cttcttgaag aacttataat attaaatctt gaaaatatta atcctgctac tcatcaatta
      181 aaagagctat acacagatga aaaacttgtc aaagaaacta agtacaaaga gataattgag
      241 cacacagaat tgttttttga gaatgaacct aagataggcg gatttaacct aatatccttt
      301 ctccgtaagc ctattattca aagtccatat aacattgaag agcagcttga tttcattcgt
      361 gcaaactgga gttcattcat cgatgagaca ttattaagta aaattctttc cggcaaagat
      421 ctgatacacg aagattataa gttgttcgta cagcacggcg gcggagaaaa aggcacaccg
      481 cccgttcccg cttacgaatt tgattataaa tattttgaat cattaaaatt aaaacttaaa
      541 gaaggtcaaa aactttctcc tgaagaatac gagtattatc aactcgaagt aaatcggttt
      601 acagttgata ttgattggat gccccgggtt gtgatgattg caaaaaatgc ttttgtatgg
      661 cttcatcagc tatctaaaaa atatggcaga gaaataaaaa ctttggatca gattccagat
      721 atagagcttg atacacttgc tcgatggaat tttaccgcac tttggttaat aggaatttgg
      781 gaacgaagtt cggcatcaag aaaaataaaa caaatgatgg gtaaccccga agctgcatca
      841 tcagcatatt ctttatatga ttatgtaatt gcaaatgaac ttggcggtga atctgccttt
      901 gagaatttaa aagctcgggc ctggcaaaga ggaataagat tatcgagtga tatggtaccg
      961 aatcatacag gaatttactc aaaatgggtt actgaaaaac cagaatactt tattcaaaac
     1021 aaagttccac cctatccaaa ctataaattt accggaccaa atctttctga tgatgatcgt
     1081 gttgaagtaa gagttgaaga ccaatattat tcaagaactg acgcagcggt tgtgtttgaa
     1141 agacttgatc gtttaaccgg agaccgcact tacatttatc atggtaacga tggaacaaat
     1201 atgccgtgga atgacactgc acaattggat ttgatgaaag ctgaagttcg tgaatcactt
     1261 attcagacaa ttatgcacgt tgcacgcaaa actccgatta ttagatttga tgctgcaatg
     1321 acacttgcga aaaaacatta tcaacgatta tggtttccaa ttcctggttc cggcggagca
     1381 attccatcac gtgctgatta tgcaatgaca cgatcgcagt ttgatgctat tatgccggaa
     1441 gaattctggc gtgaagttgt ggatagaata aatgcagaaa tgcctgatac acttttgcta
     1501 gcagaagctt tctggctgat ggaaggatat tttgttcgca cacttggtat gcatcgtgtt
     1561 tataatagcg catttatgca tatgtttatg aaagaagaga acgaaaagta taagttgctt
     1621 atcaaaaaca ctctggcatt taatcctgag attttaaaac gttacgtcaa ttttatgagc
     1681 aatcccgatg aagagactgc aataaatcaa tttgggaaag gtgataaata ttttggggtt
     1741 gcaacaatgc ttgtaacatt gcccggtttg ccaatgtttg cacacggaca aattgaaggg
     1801 ctttccgaaa aatatggaat ggaatacaag cgggcttact atgacgaatt tacagatgat
     1861 aatttggttg ccagacacga aaaagaaatt tttcctctaa ctaaaaaaag atatttgttt
     1921 agtcaggtta ataattttga gttgtatgat tttatggacc caaatggaat ttgcaacgaa
     1981 aatgtttttg catttactaa caaggacggc aatgaagtat cactcgttgt ttacaacaac
     2041 tcatacagcg aagcaatagg aacaataaat ttaagctgcg aacgtgctta tggtgaagaa
     2101 ggcagaacga gcacaactac tattgataaa gcacttggaa taaattacgg ttatggattt
     2161 tactacatct tcaaagacta catacaacag cttgaattta tagtatccgg ttcagaaata
     2221 agttcaactg gatttttctt tcacctgatg ggttatcaac gaaaggtact tctggacttt
     2281 agagaagttt acgatactga tggaagatat acagtacttt atcaacagct taatggaaga
     2341 ggcgtaaatt ctattgagcg tgcattaagc gaaatgaacc ttgcaccatt tcataattcg
     2401 cttgtacatt tttttacatc aatcaatttc agcagattac ttttatcttt ggatttttat
     2461 aataaggatg agattgaaat accggaagta gatgaagagt taacgagaat aattaaatcg
     2521 ttaaacaaaa ttaaatcgat agaaacatca ttatctgaac tgacagctca aattcaatct
     2581 gatatttctg atatcagtcg ctttaataaa ttatttttag aaatctcatc aaccaaacga
     2641 ccagctaaat ggattggtga tgttaaaaat attttatatc ctcaaaatga tattgagcga
     2701 aagaggttat tatcttttct attgttaaat aaaataata
//
LOCUS       DASWBY010000100         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_342102, whole genome shotgun sequence.
ACCESSION   DASWBY010000100 DASWBY010000000
VERSION     DASWBY010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342102"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /gene="secD"
                     /locus_tag="VF870_02015"
     CDS             <1..1390
                     /gene="secD"
                     /locus_tag="VF870_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX9250982.1"
                     /translation="RNRVDQYGVSEPSISRQGSRRIIVELPGVAKEEEAKQLLQGTAL
                     LEFRLVKDAELAYPIMQRIDDVLAKRNKSGVKDSLGNEIAALDTTKKVTDTTSASDTT
                     KQLSGEEFKAQHPFFSAAVINPQSPYADAYVSKDDRNKITYWLSLPEVQKVIPDNVEF
                     VFSAKPINSKDGKQIFIMYLVNKTPELTGGVVTNAQANIDPNTSSPIVNMEMNSEGAT
                     DWARITGANVGKRIAIMLDGVVFTAPNVKGKIPGGRSQIEGSENIEEAKLLEIVLKAG
                     ALPAPVDVIEERIVGPSLGQDSISAGFSSAMIGYLAVGLFMIFYYRQAGTVSALTLVL
                     VILFILGILAGFSATLTLPGIAGIVLTIGMAVDANVLIFERIREEMATGKTIKASIDS
                     GFSRAYSAIIDSNITTFFTGIILYQFGTGPVQGFALTLMIGIAASLFGALVITRLAID
                     ILVSRGTKLSIG"
     gene            1418..2560
                     /gene="secF"
                     /locus_tag="VF870_02020"
     CDS             1418..2560
                     /gene="secF"
                     /locus_tag="VF870_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX9250983.1"
                     /translation="MRIFHNLNVDFLSKRKFFYVFSLSLFLIGVISVIFKGLSFGIDF
                     KGGSEIVLQFQKQINVAEVRKDIENIGLGNVEVRTFGGETGALIRTELQQLPENVYPN
                     VVKAIESEIKIIVPNVAFNVVEKTPTSITYSFPNADTANLVADKLFEAGFQSGKVSEE
                     PDNTQIDVSVGISEWIKENLREKIQDNPFKVVKEDRVGPKIGDELKRDAVIAVFLSLV
                     VILIYLGFRFKFVFAVGAVAALFHDVLITLGLYAALYGVIPGLNLDIDLSVVAAFLTL
                     VGYSINDTVIVFDRVRENMKIHKTLPLLEVINKSINQTMSRTIITAFTTLITVFILLI
                     LGGDVLRAFAFTLFFGIIIGTYSSIFVASAFVLEYAERTKKKVQFS"
     gene            complement(2651..>2835)
                     /locus_tag="VF870_02025"
     CDS             complement(2651..>2835)
                     /locus_tag="VF870_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250984.1"
                     /translation="CCGGFFIDINDSTYRFFTIPEGSNLSLENPVFPIYVKLDWAKAD
                     TLCLGDEIKVLRIEKR"
BASE COUNT          920 a          477 c          535 g          903 t
ORIGIN      
        1 aagaaacaga gttgaccagt acggtgtttc tgaaccatca atttccagac aaggaagtag
       61 aagaattatt gtagaactcc ccggtgttgc aaaagaagaa gaagcaaagc agcttttgca
      121 gggcacagca cttttggaat tcagacttgt aaaagatgct gagttagcct atccaattat
      181 gcaaagaatt gatgatgttc tggctaagag aaataagtca ggtgttaaag attcacttgg
      241 aaacgaaatt gctgcgttag atactacaaa aaaggtgact gacactactt cagcatctga
      301 cacaactaaa caattatcag gagaagaatt taaagcacaa catcctttct tttctgctgc
      361 ggtgataaat ccacaaagcc cttatgctga tgcatatgtt agtaaggatg acagaaacaa
      421 gatcacatac tggttaagcc tacctgaagt acaaaaagta attcctgata acgttgaatt
      481 tgtattttca gctaaaccaa ttaactcaaa ggatggcaaa cagattttca taatgtattt
      541 ggttaacaaa accccggaac ttactggtgg tgttgtaacg aatgctcagg ccaatattga
      601 ccctaataca tcttcaccta ttgtaaatat ggaaatgaat tctgaaggtg caaccgattg
      661 ggccagaata actggcgcaa acgtcggaaa aagaattgca ataatgcttg atggtgttgt
      721 atttactgca ccaaatgtaa aaggcaaaat tcccggaggt cgttcacaga tagaaggatc
      781 agaaaatata gaagaagcaa aactgcttga gatcgttctt aaagccggtg ctctgcctgc
      841 ccctgttgat gtaattgaag aaagaatcgt tggtccatca ttaggacagg attctatatc
      901 agctggattc agttctgcta tgataggtta ccttgctgtc ggcttattta tgattttcta
      961 ttacagacaa gcaggaacag tttctgctct tacccttgtg cttgttatac tttttatact
     1021 tggtatttta gcaggattta gtgcaaccct aacattaccc ggtattgcag gtattgtgct
     1081 cacaattggt atggcagtgg atgcaaatgt tttgatcttt gaaagaatac gagaagagat
     1141 ggcaacaggt aaaacgatca aagcctctat tgacagcggt ttttccagag cttattcagc
     1201 tattatcgac tcaaatatca caacattttt tactggaatt attctttatc agtttggtac
     1261 cggcccggtt caaggttttg cacttacact aatgattggt attgcagctt cattatttgg
     1321 agcattagtt attacaaggc ttgctataga tatactagtt tcaagaggaa ctaaactcag
     1381 cataggttaa attttttatt aaggagttaa tttttcaatg cgaatatttc ataatttaaa
     1441 tgtggatttt ttaagtaaga gaaaattttt ctatgtgttt tctctttctt tatttttaat
     1501 cggagtaatt agtgtaatct ttaaaggatt aagctttgga atcgatttta aaggcggatc
     1561 cgaaattgtt ctgcagtttc aaaaacaaat caatgttgca gaagtaagaa aggatattga
     1621 aaacataggc ttgggtaatg ttgaggttag aacatttggc ggtgaaaccg gagcgttaat
     1681 tagaacagag cttcagcaat taccagaaaa cgtttacccc aatgttgtta aggcaattga
     1741 aagtgaaata aaaattattg ttcccaacgt tgcttttaac gtagttgaaa aaacaccaac
     1801 ctcaattact tactcattcc caaatgcaga tacagcaaat ttagtcgccg acaaactttt
     1861 tgaagccggt tttcagtcgg gtaaagtttc cgaagagccg gataacactc agattgatgt
     1921 aagtgttggt atttctgaat ggattaaaga aaatctaaga gaaaaaattc aagataatcc
     1981 atttaaagta gtcaaagaag atcgtgtggg tcctaaaatc ggagatgaat taaaaaggga
     2041 tgccgttatc gccgtttttc tatcactggt agttatactt atttatctcg gatttagatt
     2101 caaatttgtt tttgctgttg gtgcagttgc tgcacttttc catgatgtat tgattacact
     2161 tggactttac gcagctttat acggagttat tcctggatta aacctggata tagatctttc
     2221 agttgttgca gcttttctaa cacttgttgg ttactcaatc aatgatactg taattgtatt
     2281 cgatcgtgtt agagagaata tgaaaattca taaaaccttg cctttacttg aagtaataaa
     2341 taaaagcatc aatcaaacga tgagcagaac tattattact gcttttacaa cattaattac
     2401 tgtatttatt cttctaattt tgggcggcga cgttttaaga gcatttgctt ttacactttt
     2461 cttcggtatc atcattggta cctactcttc aatcttcgtt gcaagtgcat ttgtacttga
     2521 atatgctgaa agaactaaga agaaagttca gttttcataa gcgcaatttc ttaaaaattc
     2581 aaaagccgat ggagcaattc accggctttt ttaattttaa actgttccta aaaaaacaat
     2641 taaatattgc ctaccgcttt tcaattctaa gcacttttat ctcatcaccc aaacaaaggg
     2701 tatcggcttt tgcccaatct aatttaacat aaattggaaa gacaggattt tccagagata
     2761 aattacttcc ttcaggaatt gtaaaaaacc tataagttga atcatttata tcaataaaaa
     2821 atcccccgca gcaac
//
LOCUS       DASWBY010000101         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_117006, whole genome shotgun sequence.
ACCESSION   DASWBY010000101 DASWBY010000000
VERSION     DASWBY010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117006"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF870_02030"
     CDS             complement(<1..234)
                     /locus_tag="VF870_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250985.1"
                     /translation="MKVKELILIVVFFSSSVFSQTEISLGGYLGGGSFSGNSVSVGGF
                     TTSIFVEANLPLFEEVFPRVSLIYTKDFNAILPN"
     gene            301..657
                     /locus_tag="VF870_02035"
     CDS             301..657
                     /locus_tag="VF870_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX9250986.1"
                     /translation="MEKILYHYKAKVINVYDGDTCTVDIDLGMHIWNKGEKLRLNRIN
                     APELRVNEKAKGLKSRDFLRSLILDKEVYIETIKDKKEKYGRYLAEIWLEDEKNNFIN
                     INDLLVKGGFAKYQKY"
     gene            680..1018
                     /locus_tag="VF870_02040"
     CDS             680..1018
                     /locus_tag="VF870_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HEX9250987.1"
                     /translation="MSRTSKINFIIQLDDKKMPEKIEWYADDADFEGMKEAKTMMLSL
                     WDKEDKVTLGIDLWTNDMLVDDMNLHYYQMILKLGDTYRKATSNPDTAKMFDEFASSF
                     ADKIKLFDKK"
     gene            1134..2492
                     /locus_tag="VF870_02045"
     CDS             1134..2492
                     /locus_tag="VF870_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX9250988.1"
                     /translation="MDVEILSRLQFALTIAFHYIYPPLSIGIAIVLVIMEGMYLKTKD
                     KFFENMTKFWVKVFALTFAIGVATGIVMEFEFGTNWATYSRFVGDVFGSALAAEGIFA
                     FFLESGFLAVLVFGWEKVGPKMHFFSTIMVSLGSMFSAVWIVVANSWQQTPAGFHIVG
                     EGINARAEITDFWAMVFNPSSMDRLSHVLSGCWLAGAFLIISVSAYYLLKDRHIRFAK
                     ASIKIALVVVLIASLFQLFTGHRSAVGVSKNQPAKLAAMEAVFDDQTNAPLYLFGWVD
                     SENQKVNFSIAVPGMLSYLIGFDTDTKVTGLNSFKKEERPPVNTVFQAYHLMVAIGFT
                     LIGISLLGIFFWWRGTLFKQRWMLWIFVFSVLLPQIANQIGWITAEVGRQPWIVYGLL
                     KTSEGLSKAVEAGQVWFSLILFTVIYIGLFILFIYLLNEKIQHGPEDVDSIPSEIGHP
                     KA"
BASE COUNT          866 a          358 c          502 g          868 t
ORIGIN      
        1 atttggcaga attgcgttaa aatcttttgt gtaaataaga cttactcttg gaaagacttc
       61 ttcaaaaaga ggaagattag cttcaacaaa tatggaagta gtaaatccac caacagaaac
      121 tgagttacca ctgaatgatc caccaccaag atagcctccg agggatattt cagtctgtga
      181 aaacactgat gatgaaaaga atactacaat taggattaat tcttttactt tcaagtttta
      241 tccctttaaa ttagatgtaa caaaaatata ggcaagcgat acaaatcgaa aggaatgaat
      301 atggaaaaaa ttctttatca ttataaagca aaagttatta atgtttacga tggcgatacc
      361 tgcactgttg atattgacct tggtatgcat atctggaata agggtgagaa gcttagatta
      421 aatagaatta atgcacctga attaagagtt aatgaaaaag caaaaggatt aaaaagtcgt
      481 gattttttaa gatcactaat tcttgataaa gaagtttata tagaaacaat taaagacaaa
      541 aaagaaaagt atggaagata tctggctgaa atctggttag aggacgaaaa aaataatttt
      601 atcaacatca atgatttgtt ggtaaaaggc ggttttgcaa aatatcagaa gtattaaatt
      661 ttaattacaa ggaaacaata tgagtagaac atcaaaaatt aattttatta tacagcttga
      721 tgataaaaaa atgccggaaa aaattgaatg gtatgcagat gacgctgatt ttgaaggaat
      781 gaaagaagca aaaacaatga tgctttctct ttgggataaa gaagataaag ttactttagg
      841 catcgatctc tggacgaatg acatgcttgt tgatgatatg aatctccatt attaccagat
      901 gatattaaag cttggtgata catatcgaaa agcaacaagc aacccggata cagcaaaaat
      961 gtttgatgaa tttgcctcaa gttttgcgga caaaattaag ttgtttgata aaaaataatt
     1021 ttcttgttta attgattgtc tcaatgatca aaaaacattt aaatcaatat tctttttatt
     1081 cattttcagt ttagctaatt taaaattagt atctaaaaaa ttttaggttt aatatggatg
     1141 tagaaatcct ttcccgactt caattcgctt taactatagc tttccactat atctatcctc
     1201 cacttagtat tggtatagca attgtacttg taataatgga agggatgtat cttaaaacaa
     1261 aagataaatt ctttgaaaat atgaccaagt tttgggttaa agtttttgct ctgacttttg
     1321 caattggtgt tgcaactgga attgtaatgg aatttgaatt cggaacaaac tgggcgacat
     1381 actcgcgctt tgttggagat gtttttggaa gcgcactagc tgcggaagga atatttgctt
     1441 tctttttaga atcaggtttt cttgctgtgt tggtttttgg gtgggaaaag gttggaccca
     1501 aaatgcattt cttttcaacc ataatggtct cactcggatc aatgtttagc gctgtttgga
     1561 ttgttgttgc aaactcctgg cagcaaactc ctgcaggatt tcatatcgtt ggcgaaggaa
     1621 taaatgcccg agcagagata acggattttt gggcaatggt ttttaatcct tcctcaatgg
     1681 atagactttc acatgttctt tcgggatgct ggttagcagg tgcatttctt attataagtg
     1741 taagtgctta ctatctttta aaggatagac acattcgttt tgcaaaagct tcaattaaaa
     1801 ttgctctagt tgtagtatta attgcatctt tgtttcaact ttttacagga caccgaagtg
     1861 cagttggagt aagtaaaaat cagcctgcta aacttgctgc aatggaagct gtttttgatg
     1921 atcaaactaa tgctccgctc tacctgtttg gctgggtcga tagcgaaaat caaaaagtaa
     1981 attttagtat tgctgttccg ggtatgttaa gttatttgat tggatttgat actgatacaa
     2041 aagtgacagg gttaaattca tttaaaaaag aagaaagacc acctgtaaat actgtctttc
     2101 aagcttatca tttaatggtg gctattggtt tcactttaat tggaataagt ttgcttggaa
     2161 tatttttctg gtggcgggga accctattca agcaaagatg gatgctttgg atttttgtct
     2221 tctcagtttt acttccgcaa attgcaaatc aaattggatg gataaccgca gaagttggaa
     2281 gacaaccatg gattgtttat ggattactca agacttcgga aggtttatca aaagctgttg
     2341 aagctgggca agtatggttt tcattaatcc tttttacagt aatatacatc ggtttattta
     2401 ttttatttat ttacttgctg aatgaaaaaa tacaacatgg cccggaagat gttgattcga
     2461 taccttctga aattggacat ccaaaggcat aaaagagttt aaggagtaat atggaattta
     2521 cttttgatct aaatacaata tggtttttct taattggaat tttattggtt ggatatgcaa
     2581 tccttgatgg attt
//
LOCUS       DASWBY010000102         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_680417, whole genome shotgun sequence.
ACCESSION   DASWBY010000102 DASWBY010000000
VERSION     DASWBY010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680417"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VF870_02050"
     CDS             <1..1018
                     /locus_tag="VF870_02050"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX9250989.1"
                     /translation="VRVMKFLGVKTLVVSNACGGMNPDYRKGDLMLMSDHINLLGDNP
                     LIGKNEDELGPRFPDMSEPYSLDLISKINETLEVIRKHTADNYPVGIILGTGLGGLVK
                     EINIEHQIDYADLPHFPLSTVESHHGKLIFGTINGQKVVAMQGRFHYYEGYTMQQITY
                     PVRVMKFLGVKTLVVSNACGGMNPDYRKGDLMLMSDHINLLGDNPLIGKNEDELGPRF
                     PDMSEPYSLDLIKLAEEIATENNIKVHKGVYVAVPGPNLETKAEYKFLREIGADVVGM
                     STIPENIVANHMGMKVLGMSIITDECFPETLKPVNVEEIIAAAMKAEPKMTLIMKEVI
                     KRIK"
     gene            1015..1635
                     /locus_tag="VF870_02055"
     CDS             1015..1635
                     /locus_tag="VF870_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250990.1"
                     /translation="MKFSPKKMLVNYGAAILLAAFLFISNFLNTNLFNFGQLNFAVWF
                     VLSIFSFSCGWFINRVLGWHHGGKIVFAIIIAVTIVSLFVIIFFNEYFAASELLTENI
                     ILYSLRNIMLGAMGFFGMALQEVLGSERESVILKEKIKVYEQTMLDAKKEAELTLREA
                     KVAAQKLINDAELHAKNTILKKERIEKELKEFIHTERELIKKYEEL"
     gene            1664..>2937
                     /locus_tag="VF870_02060"
     CDS             1664..>2937
                     /locus_tag="VF870_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9250991.1"
                     /translation="MFKQNLEKIGYPKLEQEVLKFWQSNKIFEKSISTRDEKKSWTFY
                     EGPPTANGKPGIHHVMARTLKDLVCRYKTLQGYRVNRKAGWDTHGLPVEIEVEKALGI
                     KHKSEVLDYGVEKYNQKCRESVFTYLDLWEKMTTRMGYWIDLKSAYITLDNNYIESVW
                     WALKTLFDKGLLYKDYKIVPQDPKSETVLSSHELALGYRETKDPSVYVLFKLINADEH
                     FLVWTTTPWTLISNVALAVGSDIDYVKIKTEHATMILAKERLSVIDKEYEILAEMKGS
                     ELAGIEYEQLMEYCDVDKKAFYVVEGDFVSTADGSGIVHIAPAFGADDYEVSKKYGLP
                     MLQPVTRGGLFTDDVTDFAGQFVKDADAGIIVKLKHEGKLYRKETILHTYPFCWRHPE
                     VPVIYYARESWFIRTTSIADRMVELNKQINWQP"
BASE COUNT          982 a          448 c          597 g          910 t
ORIGIN      
        1 tgttcgtgtg atgaaatttc tcggagtaaa aactcttgtt gtctcaaatg cttgcggcgg
       61 aatgaatcct gattaccgca aaggtgattt aatgctgatg tctgatcata ttaatcttct
      121 tggtgataat ccgcttatag gaaaaaatga agatgaactc ggtccaagat ttcctgatat
      181 gagtgaacca tattcgctgg atttgataag taaaataaat gagactttag aagtaatcag
      241 aaaacatact gctgataatt atcctgttgg aattattctt ggtactggtt taggcggact
      301 tgtaaaagaa atcaatatcg aacatcaaat tgattatgct gatcttcctc attttccatt
      361 atccactgtt gaatcgcatc atggcaaatt aatttttgga actataaacg gacaaaaagt
      421 tgtagcgatg cagggaagat tccattacta cgaaggctac acgatgcagc agattactta
      481 tcctgttcgt gtgatgaaat ttctcggagt taaaactctt gttgtttcaa atgcttgcgg
      541 cggaatgaat cctgattacc gcaaaggcga tttgatgctg atgtctgatc acataaatct
      601 tcttggtgat aatccgctta tcggaaaaaa cgaagatgaa cttggtccaa gatttcctga
      661 tatgagtgaa ccatattcac tggatctgat aaagcttgct gaagaaatcg cgacagaaaa
      721 taatatcaaa gttcataaag gtgtttatgt tgcagtgccg ggcccaaatc ttgaaacaaa
      781 agccgagtac aaatttttaa gagagattgg tgctgatgtt gtgggaatgt caaccattcc
      841 tgaaaacatc gttgcaaatc atatgggaat gaaagtgctt ggtatgagca ttataacaga
      901 cgaatgtttt cctgaaactc taaagcctgt aaatgttgaa gagattatcg cagcagcaat
      961 gaaagctgaa cctaaaatga ctttaataat gaaagaagta attaagagaa taaaatgaaa
     1021 ttctcaccaa agaaaatgtt agttaattat ggtgccgcaa ttttgctggc agcatttctt
     1081 tttatttcaa attttttaaa cacaaacttg ttcaattttg gtcagcttaa ctttgctgtc
     1141 tggtttgtgc tttcaatttt tagtttttcc tgcgggtggt ttataaatcg tgtattaggc
     1201 tggcatcacg gcggtaaaat tgtttttgca ataatcatag ctgttacaat tgttagtttg
     1261 ttcgttataa ttttctttaa tgaatatttt gcagcctcag aattgcttac agaaaatatt
     1321 atactataca gccttagaaa cattatgctt ggcgctatgg gattttttgg tatggcactt
     1381 caggaagtgc ttggttctga aagagaaagt gttatactta aggaaaaaat aaaagtttat
     1441 gaacagacaa tgctcgatgc aaaaaaagaa gctgagttaa ctttgagaga agctaaagtt
     1501 gctgctcaaa aattaattaa tgatgcagaa cttcatgcaa aaaatacgat tttgaaaaag
     1561 gaaagaattg agaaagaact taaagagttc attcatactg aaagagaatt aattaagaag
     1621 tacgaagaat tatagtctac ttattatacc acagcagtta aaaatgttta agcagaattt
     1681 agaaaaaata ggttatccga agttagaaca ggaagtatta aagttctggc aatccaataa
     1741 aatatttgaa aaaagtattt ctacccgtga tgaaaaaaaa tcatggacat tttatgaagg
     1801 tccgcctacc gctaatggaa aacccggtat tcatcatgtt atggcaagaa ctttaaaaga
     1861 tcttgtttgc cgttacaaaa ctttacaggg atacagagta aacagaaaag ccggttggga
     1921 tacacacgga ctccctgttg aaattgaggt tgaaaaagct cttggtataa aacataaaag
     1981 tgaagtgctc gattacggcg ttgagaagta caatcaaaaa tgccgtgaat cagtttttac
     2041 ttatcttgat ttatgggaaa agatgacaac ccgtatgggt tattggattg atctaaaatc
     2101 cgcatacata actcttgata ataattatat agaatcagtt tggtgggcgc taaaaacttt
     2161 atttgataaa ggtcttctct ataaagatta taaaattgtt ccgcaggatc ctaagtctga
     2221 gacagtttta tcttcgcacg aacttgcgct aggctaccgc gaaacaaaag atccatctgt
     2281 ctatgttttg tttaaactta taaatgctga tgaacatttt cttgtctgga caacaactcc
     2341 atggactttg atttcaaatg ttgcgttggc tgttggttcc gatattgatt atgtaaaaat
     2401 caaaacagaa catgcaacaa tgattcttgc aaaagaaaga ttgtctgtaa tcgataaaga
     2461 gtatgaaatt cttgctgaga tgaaaggcag cgaacttgca ggaattgaat atgaacagtt
     2521 gatggaatat tgtgatgttg ataaaaaagc tttttatgta gttgaaggtg attttgtaag
     2581 taccgctgat ggatcaggaa ttgttcacat cgcaccggca tttggtgctg atgactacga
     2641 agtttctaaa aagtatggtt tgccgatgct gcagcctgtt actcgtggcg gattatttac
     2701 agatgacgta actgattttg ccggacagtt tgtaaaagat gcagacgctg gaattattgt
     2761 taagcttaaa cacgaaggta aactttatag gaaagaaaca attcttcata catatccatt
     2821 ctgctggaga catccggaag ttcctgtaat ttattatgca cgcgaatcat ggtttatccg
     2881 cacaacttct attgcagata gaatggtcga actgaataaa cagatcaact ggcagcc
//
LOCUS       DASWBY010000103         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_266868, whole genome shotgun sequence.
ACCESSION   DASWBY010000103 DASWBY010000000
VERSION     DASWBY010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266868"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VF870_02065"
     CDS             complement(<1..405)
                     /locus_tag="VF870_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250992.1"
                     /translation="MKHYNIIAAVILLLVILFQIGCVQYHDQPQHKDEKEKLVLDLSG
                     RWKFSLGDDQRWKDVRYDDRNWEKIYAPSSWENQGFHGYDGYAWYRNSFKLTSDLKTK
                     DFYLVLGFVDDVDQTFINGNLIGVSGGFPPDYR"
     gene            569..1777
                     /locus_tag="VF870_02070"
     CDS             569..1777
                     /locus_tag="VF870_02070"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX9250993.1"
                     /translation="MKNLYISFLAVVFIGNAFPQNQTVDSTLWKKAEQLCKKFTLVDT
                     HIDLPDWLYDEWFDVTQPGKGEIDYPRAIVGGLDVPFMSVYTSPGLEGTGKSKIKADS
                     MISLVHKMAVKWPDKFTFVQSTSDIENNLNKDKILLTIGMENGSPIESNLNNLKEFYD
                     KGVRYITLAHYKWNHICDSANDPERKWNGLSPFGENVVSEMNRLGIMVDISHVSDSTF
                     YDVIKLTKAPVIASHSCCRIFTPGYERNMSDEMIKVLAENGGVIQVAFASFFLRNDIY
                     QQYTDGEERIKEYIKQNNLKSGSDSAWQYEEKYWKENPLPNATVKDAADHIDHIKNLV
                     GIDYVGLGSDFDGTGGLLPVDLNDVSKYPNLVYELLVRGYTNEDIKKVLGENLLRVWK
                     RVEDVSKKLN"
     gene            complement(1928..2368)
                     /locus_tag="VF870_02075"
     CDS             complement(1928..2368)
                     /locus_tag="VF870_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9250994.1"
                     /translation="MKTTFLHKAEKLYFLLAAILFVLLVTDSFGQTQKISEVSKHKFA
                     LQNLEMGIQSENEGVRESSIYLAGQYRFIDTEDALIKQLKVEKNSDIKVLIGLALFRM
                     NSGKGMNELQRLASIDENSRVRRMSYAIYNEYQVNNSNRTAIVK"
     gene            complement(2547..3233)
                     /locus_tag="VF870_02080"
     CDS             complement(2547..3233)
                     /locus_tag="VF870_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9250995.1"
                     /translation="MPKILVVDDEQNMRTGLKDNLEFEGFDVETANDGEQGLKKILEN
                     TYNLIILDVMMPKKSGFDVCKEVRKTGITTPIILLTAKGEEIDKVVGLEIGADDYVTK
                     PFSLRELLARVKAILRRGENLVMNETDREVKIGRLDVNFNGYKASSKNIDVPMSHKEF
                     EILNYLWKHRNSTVSRDDLLTEIWGYDENPNTRTVDNFILKLRQKVEIDSNHPQVILT
                     VHGIGYKLVL"
BASE COUNT         1099 a          559 c          505 g         1143 t
ORIGIN      
        1 tcgataatcg ggaggaaatc cacctgaaac accaatcagg tttccattaa taaaagtctg
       61 atcaacatca tcaacaaaac ctaaaaccag ataaaagtct ttcgttttaa gatcagaagt
      121 caatttaaaa gaatttcggt accaggcata accatcataa ccatgaaatc cctgattttc
      181 ccaagaagaa ggggcataaa ttttttccca attcctgtca tcatatctta cgtctttcca
      241 tctctgatca tcaccaagac taaatttcca tcgtccggat agatctaata ccaatttttc
      301 tttttcatct ttatgctgcg gttgatcatg atactgaacg catccaatct gaaaaagtat
      361 aactaataat agaataacag ccgctatgat attataatgt ttcatataca tattttgttt
      421 ttttctagaa caaacctaat tgattcattc tttaacttca tcacaattaa gtaaacattt
      481 gtaaaaaatt gtcatgattt ccatttgaga gatagttgaa attaactatt ttctaattga
      541 tttatataca tattcttaat gagcataaat gaaaaatctt tatatttctt ttttagccgt
      601 agtttttatc ggaaatgctt ttccacaaaa tcaaactgtt gattctacac tttggaaaaa
      661 agcagaacaa ctatgcaaaa aatttacatt ggttgatact cacattgatt taccggattg
      721 gctttacgat gaatggtttg atgtaacaca acctggtaaa ggtgagattg attatccgcg
      781 cgcaatagta ggcggacttg acgttccatt tatgtcagtt tatacttcgc cagggttaga
      841 aggaactggt aaatcaaaaa ttaaagctga ttcaatgatt agtcttgttc ataaaatggc
      901 tgtaaaatgg ccggataaat ttacttttgt tcaatctact tctgacatag aaaataatct
      961 taataaagac aaaatcttat tgacaatagg aatggaaaat ggctcaccta ttgaaagcaa
     1021 tcttaataac ttaaaagagt tttatgataa aggtgttcga tacataactc ttgcacatta
     1081 taaatggaat cacatttgtg attctgctaa tgatcctgaa agaaaatgga atggcttaag
     1141 tccatttgga gaaaatgttg taagcgaaat gaatcgttta ggaattatgg ttgatatttc
     1201 tcacgttagc gacagcactt tttacgatgt gattaaatta acaaaagctc ccgttatagc
     1261 ttctcattca tgttgtagaa tatttacccc tggttatgaa cgaaatatga gtgatgagat
     1321 gataaaagta ttagctgaaa atggcggagt aatccaggta gcatttgcaa gcttttttct
     1381 tcgcaatgat atttatcagc aatacacaga tggagaagaa cgaataaaag aatatataaa
     1441 acaaaataat ttaaaatctg gttctgacag tgcctggcaa tacgaagaaa aatattggaa
     1501 ggaaaatccg ctccctaatg caactgtaaa agatgctgca gatcatattg atcatataaa
     1561 aaatttggtg ggaatcgatt atgtgggcct tggttctgat tttgatggaa ccggtggtct
     1621 gcttccggtt gatttaaatg atgtatccaa atatccaaat cttgtttatg agcttttagt
     1681 tcgtggttac actaatgaag atattaaaaa agttttgggt gaaaacttgt taagggtctg
     1741 gaaacgggtt gaagatgttt cgaaaaaact gaattagtag taattgtaat ttcgaaagat
     1801 ccgctgtggc ggattcgagt gaagcaatct aaattttact agcaaatttc aatcacttca
     1861 tcccgattga ctatttttta aggcattatc gggactcgtg atgacaatat tataatacaa
     1921 cttctcatta cttaacaatc gcagttctat ttgaattatt aacctgatac tcattataaa
     1981 ttgcatagct cattcttctt actcttgagt tttcatctat cgaagcaagt ctttgcaatt
     2041 cattcatacc cttcccgctg ttcattctga agagtgctaa accaattaat acttttatat
     2101 cagaattttt ttcaacttta agttgcttaa ttagagcatc ttcagtatcg atgaatctat
     2161 attgtcctgc aaggtaaatc gaactttctc tgactccctc attttcagac tgaattccca
     2221 tctcaagatt ttgcagagca aatttgtgtt tacttacttc agatatcttc tgggtctgac
     2281 caaaagaatc tgtaaccaat aaaacgaaaa gtatagctgc taataagaaa tataactttt
     2341 ctgctttgtg taaaaatgtt gttttcattt tttgtacctc ttttaattaa gtaataaata
     2401 caattaagtt tctggtacaa acgtacatca atgaattgta agcggtgtca tctcaaagta
     2461 aagagatgta aagaaatgtc atgataatga aaaatgtaga atttagaatt gaatacaaaa
     2521 gatatattta ttgaatattg atcattttat aaaaccaact tatatccaat cccatgaact
     2581 gttaaaatta cctgcggatg attcgaatcg atttctactt tttgcctaag ctttaaaata
     2641 aaattatcta cagtccgggt atttggattt tcatcatacc cccaaatttc tgttaataaa
     2701 tcatctctgc tcactgttga attgcgatgt ttccataagt aattaagtat ctcgaattct
     2761 ttatgagaca ttggcacatc tatatttttt gatgaagctt tgtagccatt aaaattaaca
     2821 tccagtctgc caattttaac ttcccgatct gtttcgttca taacaagatt ttcacctcgt
     2881 cgtaaaattg cttttactct ggcaagtaat tcccttaagc tgaaaggttt tgtaacataa
     2941 tcatctgcgc ctatttccaa tccaacaact ttatctattt cttcaccttt agctgtaagt
     3001 aaaataattg gagttgttat tccagttttg cgtacttctt tgcaaacatc aaaaccggat
     3061 ttcttaggca tcataacatc aagaataatc aagttgtaag tgttttcaag aatttttttc
     3121 aaaccttgct caccatcatt tgcagtttca acatcaaatc cttcaaattc aagattatct
     3181 tttaaccctg ttcgcatatt ttgttcatca tcaacgacta aaattttagg catttttatc
     3241 tccattaatt aactgagaat ttttaggaaa cactaactta aaactacttc cttcaccgat
     3301 tttact
//
LOCUS       DASWBY010000104         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_266876, whole genome shotgun sequence.
ACCESSION   DASWBY010000104 DASWBY010000000
VERSION     DASWBY010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266876"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1604
                     /locus_tag="VF870_02085"
     CDS             78..1604
                     /locus_tag="VF870_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9250996.1"
                     /translation="MKNKNSDIELLSAYVDGELSAAEKKYIEAKIKSSLELQKELSDL
                     KKLKDLTANSYDQISESPFFETRLFANLNSDSKSKFNIKKWIPISALTLLTLALMAVL
                     KFNPNLIDNLIKQQESNLVGFYKENLRPLLYAADLTNEDIFNFAVNQELPLDNTNNQI
                     LKLGYDVKGKEYFEIKKAKQITETNNLQKFVAALDFDKDETKKIDSIINSYSNQISAL
                     VLVNDKNAVAINPNIWNTRKLILADILAFAEKHASPNFNKMIPNQVMQFDDHSVAKWV
                     NKAKSTRDDQYIFCTPDSIFTENFVFDMSEFEKNMEKMTENLTELNEQKGVLREYHFK
                     VDSSNNRAKKDANWTKQFQVSVDTNLIKVSIQNFAVDIPEFNIPNFDSIANVILEATQ
                     NVRSVRPPAPPITVGNNSYNYDYNTVIPKKKGRTDIDLDSLMHQKNIQNEKNRIEQKE
                     RAKNDSLILMQNQELKNEMDKLRKELQKFREEIKDPQMKSNDGAIKKDTSNDADIFEI
                     "
BASE COUNT          649 a          240 c          253 g          509 t
ORIGIN      
        1 actgtaaaat caagaatttt cagagcaaga gaatctataa aaaaaatact taaacctctt
       61 gaaaaagagt tgatgttatg aaaaacaaga atagcgatat cgaattactt tcagcttatg
      121 tagatggtga attatctgca gctgaaaaaa aatatattga agcaaaaatt aaatcttctt
      181 tagagttaca aaaagaatta tctgatttaa aaaaacttaa agatcttact gcaaattcat
      241 atgatcagat ttccgaatca ccattttttg aaacaagatt atttgcaaat cttaattcag
      301 attccaagtc aaaatttaat atcaaaaagt ggatccctat ttctgctctt actctgttaa
      361 ctttagctct tatggctgtt ttaaagttta atccaaacct gatagataat cttattaaac
      421 agcaggaatc aaatcttgtt ggattttaca aagaaaattt acgtccttta ttatatgcag
      481 ccgatcttac taatgaagac atttttaact ttgctgttaa tcaggaactt cctttagata
      541 acacgaataa tcaaatttta aaacttggtt atgatgttaa gggtaaagaa tactttgaaa
      601 tcaaaaaagc aaaacaaatt actgaaacta acaatctgca aaaatttgtt gctgcacttg
      661 attttgataa agatgaaact aaaaaaattg actcaatcat aaattcttat tcaaatcaga
      721 tatctgcttt ggttttagtt aatgataaaa atgctgtagc aataaatcca aatatctgga
      781 atacaagaaa attgatttta gctgatatcc ttgcgttcgc agaaaagcat gcatcaccaa
      841 actttaataa aatgatccct aaccaggtta tgcagtttga tgatcactcc gttgcaaaat
      901 gggtaaataa agccaaatca acgagagatg atcagtacat tttctgtaca cctgattcca
      961 tctttacgga aaactttgtt tttgatatgt ctgaatttga aaaaaacatg gaaaaaatga
     1021 ctgagaattt aactgaattg aatgaacaaa aaggggtttt gcgtgagtat cattttaaag
     1081 ttgattcatc gaataacaga gcaaaaaagg atgcaaattg gacaaaacaa ttccaggttt
     1141 ctgttgatac taatctgatt aaagttagta ttcagaattt tgctgttgat attcctgaat
     1201 ttaatattcc aaattttgac agtatagcca atgtaattct tgaagccacc caaaatgtca
     1261 gatctgttcg tccaccggct cctccgatta cggttggtaa taatagttat aattacgatt
     1321 acaatactgt tattccaaag aaaaaaggca gaacagatat tgatctcgat tctttaatgc
     1381 atcaaaaaaa tatacaaaat gaaaaaaatc gtattgaaca aaaggagaga gcaaaaaatg
     1441 atagtttaat acttatgcag aatcaggaac ttaaaaacga aatggataaa ttgaggaaag
     1501 aactgcaaaa attcagggag gagatcaaag atccacagat gaaaagtaat gatggtgcta
     1561 ttaaaaaaga cacctcgaac gatgcagata tttttgagat atgatcccgg ttatataatt
     1621 ttctcaaaac tcacactcag tataaaatat t
//
LOCUS       DASWBY010000105         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_154310, whole genome shotgun sequence.
ACCESSION   DASWBY010000105 DASWBY010000000
VERSION     DASWBY010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154310"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VF870_02090"
     CDS             complement(<1..1230)
                     /locus_tag="VF870_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9250997.1"
                     /translation="MKSNMPNDLKSKELLTRGVKSSEILTGNSNLLHPEKGNKFLNPS
                     KIEILSVKMFSLEESLQSQKRTYLLQFDQPRTSYIAAEIALKNPDSNNKESVLRGVTI
                     WYLEDEEVGRNNFNLEIKKDWELVEFVQSWGTPLPGFWKQGEGRLEVILENNLVLKQV
                     FYIGNSEIFDFDSEENAVAKISNKLSTLQPKKIEQLNKSFLENSSLPSLFEEFDNYVG
                     LKNLKQSLKDFITYLDFISERKKNGIETDESISANCIFLGNPGTGKTSIARLLGKFFK
                     AIGLLENGHVIEVDRADLVGEYIGETAQKTDKVINKALGGILFIDEAYTLKRENAGND
                     FGQEAIDIILKRMEDYKNKFFVIAAGYPALMQNFLESNPGLKSRFTHYFTFDDYSPKE
                     LTEIFRIFASKEKYIISK"
     gene            complement(1368..>2398)
                     /gene="groEL"
                     /locus_tag="VF870_02095"
     CDS             complement(1368..>2398)
                     /gene="groEL"
                     /locus_tag="VF870_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9250998.1"
                     /translation="TDTESMETILEDPFILIHDKKISAMKDLLPVLEKVAQQGKAMLI
                     ISEDLEGEALATLVVNKLRGTLKVAAVKAPGFGDRRKEMLQDIAILTKGVVISEEQGY
                     KLENATLEYLGKAKKVVIDKDNTTIVEGAGKTDTIKKRINEIKAQIDKSTSDYDKEKL
                     QERLAKLSGGVAVLKIGASTEVEMKEKKARVEDALHATRAAVEEGIVAGGGVALVRAS
                     NVLDKLTGENLDQTTGIKIVQKALEEPLKQIVENAGLEGSVVLWKVKEGKDDFGFNAA
                     TEKYENLIKAGVIDPTKVTRTALENAASVSSLLITTEAVVFEKKEKEPAMPPMPHGGG
                     MGGMDGMY"
BASE COUNT          702 a          470 c          343 g          883 t
ORIGIN      
        1 ctttgaaata atatactttt ctttacttgc aaaaattcta aatatctcgg ttaactcttt
       61 aggggagtag tcatcaaaag taaaataatg agtaaatctt gattttaaac ctggattaga
      121 ttctaaaaaa ttttgcatta aagcgggata accggcggca ataacaaaaa atttattttt
      181 ataatcttcc attcgtttta agattatgtc aattgcttcc tggccaaaat catttccagc
      241 gttctccctc tttagagtgt aagcttcatc aataaacagt attccgccta aagctttatt
      301 gataacctta tctgttttct gtgcagtttc gccgatatat tcaccaacaa gatcagcacg
      361 atcaacttca atcacatgcc cattttcaag aagccctatt gctttgaaaa actttcccag
      421 caatcttgct attgaagttt ttcctgtccc gggattacct aaaaatatac agtttgcaga
      481 gatgctttcg tcagtttcta taccgttttt ctttctttca ctgataaaat caagataggt
      541 tataaaatct tttaatgatt gttttagatt ttttaatccg acataattat caaactcttc
      601 aaataaagag gggagtgaag aattttcaag gaaactttta ttaagctgtt caattttttt
      661 tggctgcaga gtacttagct tatttgagat tttagcaact gcattttctt cactatcgaa
      721 atcgaatatc tcagaattgc caatataaaa aacttgttta agtaccagat tattttctaa
      781 tatgacttct aatcttcctt caccctgttt ccagaatccc ggcaaaggtg taccccagct
      841 ttgtacaaac tcaacaagtt cccaatcttt tttaatttca agattaaaat tatttcttcc
      901 gacttcttca tcttccaaat accaaattgt cactcctctt aaaacagatt ctttattatt
      961 agagtctgga tttttaagag ctatttcggc cgcgatataa cttgttcttg gttgatcaaa
     1021 ttgtaataga taagttcgct tttgagattg taaactttct tcaaggctaa acatcttgac
     1081 tgatagtatt tcaattttac ttgggtttag gaatttattt cctttttcag gatgcaatag
     1141 atttgaattt cctgttaaga tttctgaaga ttttactccc cttgtgagta attctttaga
     1201 tttcaaatca ttcggcatat ttgatttcat ttatttaata ccagtatgaa tgacaaaaat
     1261 cattttttta cagatatttc aaataccttg ccatccaaaa acatttgatt taaaacaaaa
     1321 aaggcgagga aaaacctcgc cttgtaaaat taacagagtt aaatctatta atacattccg
     1381 tccataccgc ccattccacc gccgtgtggc ataggaggca ttgctggttc tttttctttc
     1441 ttttcaaata ctacagcttc ggttgtaatt aacaatgatg aaactgaagc agcattttca
     1501 agagcagttc ttgttacttt tgtaggatcg ataacaccag ctttgatgag gttttcatat
     1561 ttctcagtag ctgcgttaaa tccaaaatca tctttacctt ctttaacttt ccagagaact
     1621 actgaacctt caaggcctgc attttcaaca atttgcttta atggttcttc aagagctttc
     1681 tgaacaattt taattccggt tgtctgatca agattctcac ctgtaagttt atcaagcacg
     1741 ttagaagctc taactaatgc aacgccgcct ccggcaacga taccttcttc aactgctgca
     1801 cgtgttgcgt gtaaagcatc ttcaactctt gctttctttt ctttcatttc aacttcagta
     1861 gaagccccga tttttagaac tgcaacacca ccggaaagtt ttgccaatct ttcctgtaat
     1921 ttttctttat cgtaatcaga agttgattta tcaatttgag ccttaatttc atttattctc
     1981 ttcttgatag tatcggtttt accagcaccc tcaacaatgg tagtattgtc tttatcaata
     2041 actacttttt tggcttttcc taaatactca agagttgcat tttctaattt ataaccttgt
     2101 tcttcagata ttacaacacc tttagtaagg atagcaatat cctgaagcat ttcttttcta
     2161 cgatcaccaa aacccggagc ttttacagcg gcaaccttta acgtaccgcg tagtttattt
     2221 actactaaag ttgccaatgc ttcgccttcc agatcttcag agattattaa cattgctttg
     2281 ccttgctgag caactttttc aagtactggc aaaagatctt tcattgcaga gattttttta
     2341 tcgtggataa gaataaaagg atcttctaaa attgtttcca ttgattcagt atcagtta
//
LOCUS       DASWBY010000106         3443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_117161, whole genome shotgun sequence.
ACCESSION   DASWBY010000106 DASWBY010000000
VERSION     DASWBY010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117161"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF870_02100"
     CDS             <1..360
                     /locus_tag="VF870_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9250999.1"
                     /translation="MSFQIRDDILDYEGKSNLIGKPVGGDIKEKKITLPLIYSLNNVP
                     VNEASRIRKILKNGNDKTKVKVVMQFVHENNGIDYALKVAKKYSDAAKESLNIFPDSQ
                     IKISMQALVDFVTERKN"
     gene            670..3435
                     /locus_tag="VF870_02105"
     CDS             670..3435
                     /locus_tag="VF870_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9251000.1"
                     /translation="MQTNHSKEVSCKALDPIFYYAKQNNVDLNNLVAGTPYNLSFLLN
                     KRERVEWSVWCKIISNARNYFSSSEFEEIGRQFVNSGSYQIGLLPAFFLFSSNKLVRI
                     LAKSIIKSGTTMFSCISQQLEFVNANNVRITVVLDEGHEFCPEFFIISKGYWGELGAQ
                     LGHKNFNLMLQIKDMKGIFDASWNEESYYFKIKRGLRWLFNIRKALQEMTESHEELLH
                     QYDKLDISKRLLQKQTTQLKTAFKISNSIRQSLNINETLKAITDTLVEVAEYSAASIE
                     LCKNMDDDEINIKYESGLTNKLISPIQKDLIVENKKIGELMVYPKPEIDYKETIELLE
                     YLIPIISIAVHDALLFNSIVDYKSNLEQKIKDRTIELQKAHQTQKRFFTNISHEFRTP
                     LTLILGPSRELREELKDEKLKTKADLIHRSASNLNKLVDELMEISKIESGELKLIASP
                     LNIVSVVNDPTLPFYSLAERRKITFNVRSDEEEIITYIDKTKFEKILTNVLSNAFKFT
                     PEGGSVDVAIHIHPEFISGSQSVSTASANIKIPKLFRDGRFVEISIKDTGVGIAEDQF
                     DKIFDRFYQVDSSHTREHEGTGIGLALTKELVELHKGIIEVESEEGKGSTFRLLLPLG
                     KDHLKPDEVKVEEKDCEDDPENEKQIPWLDAFLDSNVKHKVNVESFDKPSLLIVEDNP
                     EVRKYITGILDKYYLVLEAKDGEEGLDKSFKSIPDLIISDIMMPNLDGFQFCSKLKND
                     SRTSHIPVIMLTAKATMQDKISGLAIGADDYIMKPFEAAELKARIKNLLDQRKRLHDH
                     FQKYGLVELEGKNITSLDQKFLQRAIDIISKHISDTNFGVEVLAENLAVSRSLLLKKT
                     EALFGETPNDLIRRIRLNKAARLIEKEFGNISEIALEVGFNNPSYFADCFKKQFGVTP
                     SHYNLKK"
BASE COUNT         1262 a          517 c          608 g         1056 t
ORIGIN      
        1 atgtctttcc agattcgtga tgatattctt gattatgaag gcaaatcaaa tttaattggt
       61 aaacccgttg gcggtgatat aaaggaaaag aaaattacat taccgctaat ctattcttta
      121 aacaacgtcc ctgttaatga agcttccagg attagaaaga ttttaaagaa tggaaatgat
      181 aaaaccaaag ttaaagttgt aatgcaattt gttcacgaaa ataacggaat agactacgca
      241 cttaaagttg caaaaaaata ttctgatgct gcaaaagagt ctttaaacat tttccctgat
      301 tctcaaatta aaatttcaat gcaggctctt gttgattttg taactgaacg aaaaaattag
      361 acctaatcat tttcacgaaa ttgtctgggc tgtcagacac ctttctcatt tccagcatta
      421 aactattttc ttcttgcaac tctcattaat tattgacaat tttactcagg ctaaaaaaga
      481 ctgggctata aatgagagtg caattttact atttcacttt cctctatctg ctgattttaa
      541 ttttgtcaca tcagacaact cctcaagcta ataccctaat atgcaattta aatgataatt
      601 tacttatgca aaaccgacta ggctgcttcg atagtgataa aaaaaataat attatcgtgg
      661 ggatcatgaa tgcagactaa tcattcaaaa gaagtatcct gtaaagcatt agatccgatc
      721 ttctattatg caaaacaaaa taacgttgat cttaataatc ttgttgctgg aactccttat
      781 aacctttcgt ttttactaaa taaaagggaa agagttgagt ggtcagtctg gtgtaaaatt
      841 atttcaaatg ccaggaatta tttttcttct tcggagtttg aagaaatcgg aagacagttt
      901 gttaatagcg gatcctatca aataggtcta cttccggcat ttttcctttt ctcatctaat
      961 aaattggtaa gaattttagc aaaatcaata attaaatccg gcacaacaat gtttagctgt
     1021 attagtcagc aattagaatt tgtgaatgca aataatgtaa ggattacagt tgttttagat
     1081 gaagggcatg aattctgtcc cgaatttttt attataagta aaggatactg gggagaactt
     1141 ggtgctcagc ttggtcataa aaatttcaac ttaatgctcc aaattaaaga tatgaaagga
     1201 atttttgatg cttcatggaa tgaagaaagt tattatttta aaattaaacg gggattgcgc
     1261 tggttattta atataagaaa agcgcttcaa gaaatgactg aatcacatga agaattattg
     1321 catcagtatg acaaattaga tatatccaaa agactattgc aaaaacaaac cacacagctt
     1381 aaaacagcat tcaaaatatc aaattctata agacaaagtt taaatattaa tgaaactttg
     1441 aaagcaatta ccgatacgtt ggtagaggta gctgaatatt cagctgcaag tattgaatta
     1501 tgtaaaaata tggatgatga tgagatcaac ataaaatatg aatcaggctt gacaaataag
     1561 cttatatctc caatccaaaa ggatttaata gttgaaaata agaagattgg tgagctgatg
     1621 gtctacccta aacctgaaat agattataaa gaaaccatcg aacttttgga atacctcatc
     1681 cccataattt ctattgcggt ccatgatgct cttttattca atagtatagt tgattataaa
     1741 agtaatcttg aacaaaaaat taaagacaga actattgaat tacaaaaggc tcatcaaact
     1801 caaaaacgat ttttcacaaa catctcacac gaattccgca cgccacttac attaattctt
     1861 ggcccttcca gagaattaag agaagaatta aaagatgaaa aattaaaaac aaaagcagat
     1921 ctaatccatc gtagtgcaag taatttaaac aaattagttg atgagctgat ggaaatatca
     1981 aaaattgaat ccggtgaatt gaaacttata gctagtccgt taaatatagt ttcagttgtt
     2041 aatgatccga ctcttccttt ttattcactt gctgaaagaa gaaagatcac atttaatgtt
     2101 cgttcggatg aagaagagat cataacctat attgataaaa ctaaatttga gaagattctt
     2161 actaatgttt tatcaaatgc atttaaattt acacctgagg gtggttcggt tgacgttgca
     2221 atacatattc atcctgaatt tatttcagga tctcaatctg tttcaactgc ttcagctaat
     2281 ataaagatcc ccaaattatt tcgggatggc aggttcgtcg aaatatccat caaggatacg
     2341 ggagttggta tcgctgaaga tcagttcgat aaaatatttg accgctttta tcaggtcgat
     2401 agcagccata caagagaaca tgagggtaca ggaataggat tagcattaac aaaagaatta
     2461 gtcgaacttc ataagggtat tatcgaagtt gaaagcgagg aaggaaaggg atcaactttc
     2521 agattattat tacctttagg taaagaccat ttgaaaccgg atgaggtaaa ggtggaggaa
     2581 aaagattgtg aggatgatcc agagaatgaa aagcagattc cctggctgga tgcgttctta
     2641 gacagcaatg ttaagcacaa agttaatgtt gaatcatttg ataagccttc attacttata
     2701 gtagaagata atccggaagt aagaaaatat ataactggta tacttgataa gtattatctt
     2761 gttcttgaag ctaaagatgg tgaggaagga cttgataaat catttaaaag tattcctgat
     2821 ttaattataa gtgatattat gatgccgaat ttggatgggt ttcagttttg cagtaaacta
     2881 aaaaacgatt cacgcacaag ccatattccg gtcataatgt taaccgcaaa agcaacaatg
     2941 caggataaga taagcggact ggcaattggc gctgatgatt atataatgaa accatttgag
     3001 gccgcagaac tgaaagcaag aattaaaaac cttcttgatc aaagaaaaag attgcacgac
     3061 catttccaaa agtatggatt agttgaactg gaaggaaaaa atattacgtc tcttgatcag
     3121 aaatttttac aaagagctat tgatattata agtaaacaca tttcggatac taattttgga
     3181 gttgaggttc tggcagaaaa tttagctgta agcagatctc ttttgcttaa gaaaactgaa
     3241 gcattatttg gtgaaacacc aaatgattta atacgaagaa taagattaaa caaagcagca
     3301 agattaattg agaaagaatt tggtaatatt tcagagatag cactagaagt tggtttcaat
     3361 aacccttctt actttgctga ttgctttaaa aaacaattcg gcgtaacacc ctcgcattat
     3421 aatcttaaga aatagtttac aat
//
LOCUS       DASWBY010000107         5008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_680526, whole genome shotgun sequence.
ACCESSION   DASWBY010000107 DASWBY010000000
VERSION     DASWBY010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680526"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VF870_02110"
     CDS             complement(<1..238)
                     /locus_tag="VF870_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HEX9251001.1"
                     /translation="MITKQTKDPRTLDFLAGQVILIDKPVNWTSFNVVSRVRRLTKVK
                     KVGHAGTLDPLATWLLIVCTGKKTKEISEYQAQRK"
     gene            complement(201..572)
                     /gene="rbfA"
                     /locus_tag="VF870_02115"
     CDS             complement(201..572)
                     /gene="rbfA"
                     /locus_tag="VF870_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX9251002.1"
                     /translation="MAYRIDKVEHLIKQEISLIFLHKLSHVDLGFITITNVRVSPDLK
                     LAKIYLSVFEKEKRELVLSKIEDKKGFIRSELAHRIRIKFIPELKFFIDDTLDYVEKI
                     EGLIKKIHEDDNKTDEGSPDS"
     gene            complement(599..3232)
                     /gene="infB"
                     /locus_tag="VF870_02120"
     CDS             complement(599..3232)
                     /gene="infB"
                     /locus_tag="VF870_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX9251003.1"
                     /translation="MSETKSPKIRLYKFASEINLSSENLIEFLKTKGHVVKSHQSILT
                     EEMISEINAHFKKDIEKAEQHYKKIAEFNKKRADKSEKDETVVKIEQEPVQEEAAREI
                     VITDVPVEEEVQEVIEPGVVEEVEQKTEVVEKEPEETVPEKPETPKAPEKFVSPKSKE
                     EGRKRGLTVVGKMDLGEDKKPKPQVKRLPVTQKAPEGKLETTESAADAVKKKKRPKTK
                     KKTSTEVTPEETPVAKKRKRVKKFEIDEKDVKAAIKKTMLSMDDTATTNRASSRKKKR
                     REREAEEERLEELRTLDKNIIRVNEFIAVSELANLMNVPVGDVISKCISLGLMVSINQ
                     RLDVENITLVADEFGFEVEFQKEYTSEVLEDVKDKEEDLQPRSPVVTIMGHVDHGKTS
                     LLDYIRRTNVVAGESGGITQHIGAYRVDIGDGKQITFLDTPGHEAFTAMRARGAQLTD
                     IVVLVVAADDAVMPQTVEALNHAQAAGVPIIIAINKIDKPGANPDKIKQQMADRNVLV
                     EDWGGKYQSVQLSAKTGLNVDLLLEKIILEADLLDLKANPNRLTRGAVVEAELDKGRG
                     VTGTILVQKGTLRIGDPFVAGIHHGKVRAMFDERGNKVKEAPPSTPVLVLGFEGAPQA
                     GDTFIVLESEREAREISLKRQQLKREQDQKQIHHITLDELAKQISIGGVKELALIVKG
                     DVDGSVEALSDSLMKLSNQEVIVRVIHKGVGGISESDVLLAAASNAIIIGFRVRPNIN
                     ARKLAENQKVDVRLYSVIYDAINEVKSALEGLLSPIISEEVTGTIEIRDTFKVPKSGT
                     VAGCYVLDGKITRSNRIRLIRDGIVIHEGSIGNLRRFKDDVKEVDAGYECGLNIENFN
                     DIKVGDIIEAFKIVETKKTLA"
     gene            complement(3255..4502)
                     /gene="nusA"
                     /locus_tag="VF870_02125"
     CDS             complement(3255..4502)
                     /gene="nusA"
                     /locus_tag="VF870_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX9251004.1"
                     /translation="MNYDIVESFAQMVREKGIDKDVLGGILEEIFGLLVKKKYGEEAK
                     FDVVVNMDRGDIEIFLEREIVDVVEDPNTQISLEEVNKKGNEDDLEVGEDFVEKIELA
                     SLGRRLVVLAKQSLNQKIRELEKEIIYNEYNELLGEIVVGDIYQIRKNDILVNHNKNE
                     LILPRNEQIPFEKYKKGETIRAIVKEVRKGNSGPVIVISRSDNMFLRRLFEIEIPEIY
                     DGIIEIKSIAREPGERAKIAVESQDARIDAVGACVGMKGVRIHAIVRELNNENIDVVS
                     YSDDPIIFIQKALAPAKLKKIELDEDEKKAVVTADSDQVSLIVGRNGVNIRLAMRLTG
                     YEIEILREEKPFEEYEEDIELIDIKEELGNEVYEILINHRYDTAIEVLTAGPEKLKEI
                     EEFDDAKIEEILEIIKSQFEEEE"
     gene            complement(4519..4974)
                     /locus_tag="VF870_02130"
     CDS             complement(4519..4974)
                     /locus_tag="VF870_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251005.1"
                     /translation="MDINKENIVRICNEIAEKLNFFVIDINFRGDNRKKIIEVFVDAE
                     KNVDADNLAEISREINSVIEEQDIIKQAYRLDVSTPGVDRPLKFLKQFPKHINRNFEV
                     TYKTGEEVKIITGKLLSVEREELTFLSDKKEILIEFKNITTAKVIISFS"
BASE COUNT         1374 a         1070 c          687 g         1877 t
ORIGIN      
        1 tttttctttg tgcctggtat tcagaaatct cttttgtttt ttttcctgtg caaacaatca
       61 acaaccaggt tgcaagcgga tcaagagttc ctgcatgccc cactttttta acttttgtta
      121 accttctaac acgacttaca acattaaacg aagtccagtt aacaggttta tctataagaa
      181 taacctgccc tgctaaaaaa tcaagagtcc ggggatcctt cgtctgtttt gttatcatct
      241 tcgtgtattt tcttaatcaa gccttcaatc ttttcaacat aatcaagagt atcatctata
      301 aaaaacttta gttcaggtat aaatttaatt cttattctgt gcgctaattc ggaccttata
      361 aatccttttt tgtcttcaat tttactaaga acaagttctc ttttttcttt ttcaaaaact
      421 gataaatata tttttgccag ttttagatca ggactaactc ttacatttgt aattgttata
      481 aaacccagat ctacatgaga aagtttatgc agaaaaataa gactaatctc ttgcttaata
      541 agatgttcaa ctttatcaat tctgtaagcc atttattttc cacacgatct aaaaataatt
      601 acgctaatgt tttctttgtt tcaacaattt taaatgcttc aataatatcg ccaactttta
      661 tatcattaaa gttttcgatg tttaatccgc attcataacc tgcatcaact tctttaacat
      721 catctttaaa tcttcttaaa ttaccgatac tgccttcgtg aattacaatt ccatcacgaa
      781 ttaatctaat cctattgctt cttgtaattt ttccatcaag cacataacag ccggcaactg
      841 ttcctgattt aggaactttg aaagtatcgc gtatttcaat cgttcctgtt acttcttcag
      901 aaattattgg tgaaagcaat ccttccaacg ctgatttaac ttcgttaata gcatcataaa
      961 taacgctgta aagcctaaca tctacttttt gattttcagc taactttctt gcattgatat
     1021 tcgggcgaac acggaaacca attataatag catttgatgc ggctgcaaga agaacatcac
     1081 tttcagaaat accgccaaca cctttatgta taacgcgtac aataacttcc tgattgctga
     1141 gtttcattaa tgaatcggaa agagcttcaa cagaaccatc cacatcacct tttacaataa
     1201 gtgcaagctc tttaactccg ccgatgctaa tttgtttagc aagttcatca agagtgatat
     1261 gatgaatttg tttttgatct tgttcacgtt ttaactgctg tctcttcaaa cttatttcgc
     1321 gtgcttcacg ctcagattcc agaacaataa aagtatcgcc agcttgcggc gcaccttcaa
     1381 atccaagtac aagtaccggt gttgatggag gagcttcttt tactttattg cctctttcat
     1441 cgaacatcgc tctaactttt ccatgatgaa ttccggcaac aaacggatca ccaattcgta
     1501 atgttccttt ttgtacaaga atagttccgg ttacaccacg gcctttatca agttctgctt
     1561 ctacaactgc accacgagta agtctatttg gatttgcttt cagatcaagc aaatcagctt
     1621 caagaattat tttttcaaga agcagatcga cattcaatcc ggtttttgca gaaagctgca
     1681 ctgactggta tttgccaccc caatcttcta caagaacatt tctatcagcc atttgctgtt
     1741 ttattttatc aggatttgca cctggtttat caattttgtt tatcgcaata ataatcggca
     1801 cgcctgcagc ctgggcatga tttaatgctt caactgtttg gggcataaca gcatcatccg
     1861 cagcaacaac aagcacaaca atatctgtta actgagcacc gcgtgctctc attgccgtaa
     1921 atgcttcgtg acccggcgta tcaagaaaag taatttgttt tccatcgcca atatcaaccc
     1981 tgtatgcacc aatgtgctga gttattccgc ctgattcacc tgcaacaaca tttgttcttc
     2041 ttatataatc aagaagagat gttttaccat gatcaacgtg acccataatt gtaacaaccg
     2101 gtgatctggg ttgaagatct tcctctttat ctttgacgtc ttccaaaact tcagaagtat
     2161 attccttctg aaattcaact tcaaaaccaa actcatcggc aacaagggta atgttttcaa
     2221 catcaagtct ttgatttatt gaaaccatta aaccaagact aatacattta gaaatcacat
     2281 caccaaccgg aacattcatc aggtttgcaa gttcgcttac ggcgataaac tcattaactc
     2341 tgataatatt tttatctaat gttcttaatt cttctaatct ttcttcttca gcttctcttt
     2401 cacgtctttt cttttttctg gatgaagctc tgttagttgt agctgtatca tccatactta
     2461 acattgtttt ctttatagcg gccttaacgt ccttttcatc tatttcaaac tttttaacac
     2521 gttttctttt tttagctaca ggtgtctctt cgggcgtaac ctctgttgag gttttctttt
     2581 tagtctttgg tcttttcttt ttctttacag catcagcagc agattcagtt gtttcgagtt
     2641 ttccttcggg tgctttttgt gtaaccggaa gtcgtttaac ctgtggcttg ggttttttat
     2701 cctcaccaag atccatttta ccaacaactg ttagccctct ttttctgcct tcctctttgg
     2761 atttaggaga aacaaatttt tccggagcct tgggagtttc cggtttctcg ggaacagttt
     2821 cttcaggctc tttttcaaca acttctgttt tttgttctac ttcttcaaca acaccaggtt
     2881 caataacttc ctgaacttct tcttcaacag gaacatcagt aattacaatt tcccttgctg
     2941 cttcttcctg aacgggctct tgttcaatct taacgactgt ttcatctttt tcagatttat
     3001 ctgcacgctt tttattgaac tctgctattt tcttgtaatg ctgctcagct ttttctatat
     3061 cttttttaaa atgagcatta atctcagaaa tcatttcttc tgtaagaatt gattgatgag
     3121 atttaactac gtgtccttta gttttcagaa attcaataag attttccgaa gagagattga
     3181 tctcagacgc gaacttatat aatcttattt tgggcgattt tgtttcagac atttattagt
     3241 taccttttct taggctattc ctcttcttca aactgcgatt taattatttc taatatctct
     3301 tcaatctttg catcatcaaa ctcttcaatc tcttttagtt tttccggtcc cgcagtcaga
     3361 acttctatag cggtatcgta tctgtgattg ataagtattt cataaacttc attaccaagc
     3421 tcttctttaa tatcgattag ctcaatatct tcttcatatt cttcaaatgg tttttcttca
     3481 cgtaagattt caatttcgta gcctgtaagt ctcattgcta atcttatgtt aacaccattt
     3541 cttccaacta taagtgaaac ctgatcgctg tctgctgtta ctacagcttt tttctcgtct
     3601 tcatcaagtt ctattttctt tagctttgca ggagctaaag ctttttgaat gaaaattata
     3661 ggatcatcag agtaactgac aacatctata ttttcattgt tcagctcgcg tacaattgca
     3721 tggattctaa cacccttcat accaacgcag gcaccaacgg catcaattct tgcatcctgt
     3781 gattcaacag caatctttgc tctttcgcct ggttcacgtg ctattgattt tatttcaata
     3841 ataccgtcat aaatttcagg aatttcaatt tcaaacaatc ttcttaaaaa catattatcc
     3901 gatcttgata taactataac cggaccgctg tttccttttc ttacttcctt aacgatagct
     3961 ctaattgttt ctcccttttt atatttctca aaaggaattt gctcgttacg cggaagtata
     4021 agttcattct tgttatgatt aaccagaata tcattttttc ttatttgata aatatcgcca
     4081 acaacaattt caccaagcag ttcattgtac tcgttgtaga taatttcttt ttcaagttct
     4141 cttatttttt gattcagact ttgtttagca agcacaacaa gtcttcttcc taaagaagcc
     4201 agttctatct tttcaacaaa atcttctcca acttccagat cgtcttcatt gcctttttta
     4261 ttaacttctt caagacttat ttgcgtgttg gggtcttcaa caacatcaac aatttctctt
     4321 tcaagaaaaa tttcgatatc gcctctatcc atattaacaa ctacatcaaa ctttgcttct
     4381 tcaccatatt ttttctttac aagcaatccg aatatttcct caagtattcc accgagtacg
     4441 tctttatcaa tgcctttttc ccttaccatc tgtgcaaagg attcgacaat atcgtagttc
     4501 atttactttc tcctctttct aagaaaaact gattataact tttgcagttg ttatgttttt
     4561 aaattcaatt aaaatttctt ttttatctga aagaaaagtt aattcttctc tttctacgga
     4621 caataatttg ccggtaatga ttttcacttc ttccccggtt ttataagtaa cttcaaaatt
     4681 tctgttgata tgctttggaa attgttttag gaatttaagc ggtctatcaa caccgggagt
     4741 agaaacatct aatctgtaag cttgttttat tatatcctgt tcttcaataa cggaattaat
     4801 ttcccggctt atttcagcaa gattatcggc atcaacgttt ttttcagcat caacaaaaac
     4861 ctcaattatc ttttttctgt tgtcgcctct aaaattaata tcgatcacaa aaaagtttag
     4921 cttttctgct atttcattac aaattcgaac tatattttct ttatttatat ccatactaac
     4981 aaaaaacgcc ctcaacgggg cgaatcac
//
LOCUS       DASWBY010000108         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_342300, whole genome shotgun sequence.
ACCESSION   DASWBY010000108 DASWBY010000000
VERSION     DASWBY010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342300"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VF870_02135"
     CDS             <1..341
                     /locus_tag="VF870_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856062.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HEX9251006.1"
                     /translation="GMRVLGANFGDYKHGVFTDQVGKLTNDFFVNLLDMGTVWKPISE
                     AADTFEGRDRKTGKQKWTGTRVDLIFGSNSQLRALAEVYALDDAKEKFVKDFVAAWNK
                     VMNLDRFDLK"
     gene            complement(512..1507)
                     /locus_tag="VF870_02140"
     CDS             complement(512..1507)
                     /locus_tag="VF870_02140"
                     /inference="COORDINATES: protein motif:HMM:NF027594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HEX9251007.1"
                     /translation="MGMFSEDKLILKLVDENNFSDAAKRLFELQSEEWSMLNTGFKSL
                     ETVRLKSFQFEGYKIKAQFNAGRMTSTSAKVDPKSISERKCFLCKESLPSEQRGVLYN
                     NEYIILCNPFPIFPTHFTLTHKEHQPQRIIDTFPDMLDLSKDLSKHYTVIYNGPRCGA
                     SAPDHLHFQAGNKFFMPIDDDFHQIKNEYGKKIFEDDDLSFYAIDDKVRKFISIESID
                     KKIIINTFNRFYETYSELIDEAQEPMMNLVSFYEEEYGWRVIIFLRAKHRPAVFFAED
                     ENKMLVSPAAIDLGGVCVFPREEDFNRITKKQITEIFKEVFIDEKKLKSLVVKLS"
     gene            complement(1513..>2115)
                     /locus_tag="VF870_02145"
     CDS             complement(1513..>2115)
                     /locus_tag="VF870_02145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9251008.1"
                     /translation="KADRNKVLKEFNNSDEEIVIGMIARFSPGKGHEEFLQSAKTLSS
                     KFTNLKFFIVGEASRGEDEYANSIKGLAEKLGLRNIIFAGFRSDTPDVLSAFDIFIFP
                     SHAEAFGIALIEAMAMERASVCSNSDGVLDIAVDGETNLLFQNKNAEDLAQKTEKLII
                     DEDKRIRMGKAARKRVLEKFDIEIITDRTIEFYNNFINKN"
BASE COUNT          663 a          383 c          342 g          727 t
ORIGIN      
        1 gtggtatgcg agtcttaggc gcaaactttg gtgactataa acatggtgtg tttacagatc
       61 aggttggtaa actaacaaat gacttctttg ttaatctgct tgatatggga acagtttgga
      121 aacccatttc ggaagcggct gatacttttg aaggacgcga tcgcaagaca ggaaaacaaa
      181 aatggacagg aacgcgtgta gatctgatct ttggatcaaa ctcccaatta cgtgctctag
      241 cagaagttta tgcgctggat gatgcaaaag aaaagttcgt aaaagacttt gtcgcagcat
      301 ggaacaaagt aatgaatctc gatcgctttg atttgaagta aacaaagcat ctgtcattct
      361 aagacctgat taaatcggga cgaataatct taagttctaa acattaaggg gcatcttcac
      421 gatgcccttt ttttatgaag agaataatat tagtagtgaa tttcatatgt tgttagtctt
      481 attagtcgga ttggaggttt gataggtagt actaacttaa ttttactacc agacttttca
      541 attttttctc atcaataaaa acttctttaa atatttctgt aatttgtttt tttgtaatgc
      601 gattaaaatc ttcttcccgt ggaaaaacac aaactccgcc taaatctatc gcagcagggc
      661 ttactaacat tttgttttca tcctctgcaa agaaaacagc aggacgatgt ttagctctta
      721 gaaatattat tacacgccag ccatattctt cttcataaaa agaaacaaga ttcatcattg
      781 gttcctgtgc ttcatcaatc aattcagaat aagtttcgta aaacctgtta aatgtgttta
      841 taattatttt tttatcaatg ctttcgattg aaataaattt tctcacttta tcatcaattg
      901 cataaaagga taaatcatca tcctcaaata tttttttgcc gtattcattt tttatctgat
      961 ggaaatcatc atcaatgggc ataaagaatt tattgcctgc ctgaaaatgc agatgatcgg
     1021 gagcagaggc tccacatctt ggaccgttat aaataacagt gtaatgttta gaaagatctt
     1081 tactcaaatc aagcatatcg ggaaaagtat caataattct ctgcggctga tgttctttgt
     1141 gagttaatgt aaagtgggtt ggaaatatcg ggaaaggatt acacaatatt atatactcat
     1201 tattgtataa aactcccctt tgttcagagg gcagactttc tttgcataaa aaacattttc
     1261 tctcgcttat agatttagga tcaacttttg cggatgtaga agtcattcgc cctgcattaa
     1321 actgcgcttt aattttatat ccctcaaatt gaaatgactt taatcgcaca gtttcaagtg
     1381 atttgaatcc ggtatttagc atcgaccatt cttcgctctg cagttcaaac aaacgcttag
     1441 ctgcatcaga aaaattattt tcatcaacaa gttttagaat taatttatcc tctgaaaaca
     1501 ttcccataac tatcaatttt tattaataaa attattataa aactcaattg ttctgtcggt
     1561 tataatttca atatcaaatt tttcaagcac acgctttctt gctgctttac ccattctaat
     1621 tcttttgtct tcatcaataa tcaacttttc tgttttttga gcaagatctt cagcattttt
     1681 attttggaat aacaaatttg tttcgccatc aacagcaatg tcaagcactc catcagaatt
     1741 agaacaaact gatgctctct ccattgccat tgcttcaatc aatgcaatac caaaagcttc
     1801 tgcgtgcgat ggaaatataa aaatatcaaa agcagaaagc acatcggggg tatcactgcg
     1861 aaatcccgca aagatgatat ttctaagacc gagtttttcc gccaaacctt ttatgctatt
     1921 tgcatattca tcttcaccac gactggcttc accaacaata aaaaatttta ggttagtaaa
     1981 ttttgaggaa agtgtttttg ctgattgtag aaattcttcg tgacctttac ctgggctaaa
     2041 tcttgcaatc attccgatta caatttcttc atcactgtta ttaaactctt ttagtacttt
     2101 atttcgatct gcttt
//
LOCUS       DASWBY010000109         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_492999, whole genome shotgun sequence.
ACCESSION   DASWBY010000109 DASWBY010000000
VERSION     DASWBY010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492999"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..674)
                     /locus_tag="VF870_02150"
     CDS             complement(246..674)
                     /locus_tag="VF870_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251009.1"
                     /translation="MAGFANAQSYTEEVDLLQAAVGMDKKDMVAGFLQLAPENPFWTV
                     YDEYEAKRKELGKKRIDLLSRYAENYMNLTDAEIDAYMKEVKSLKASSDKLIDTYYGK
                     VKKASGSKLAAQFYEIENYILSTIRVEVLDNIPFFGELDN"
     gene            679..822
                     /locus_tag="VF870_02155"
     CDS             679..822
                     /locus_tag="VF870_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251010.1"
                     /translation="MAAIIKMFFFIILKFKIDNCIDVKFGHVSPAPWFEPSGIQPEVK
                     KEK"
     gene            complement(861..1253)
                     /locus_tag="VF870_02160"
     CDS             complement(861..1253)
                     /locus_tag="VF870_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251011.1"
                     /translation="MKNLLTLTAVIEFITGLLLVLLPSKFVSFLFGSALDSPVALMVA
                     NIAGVALMALGITCWIARNDNQSSAVKGVIIGLTVYNAGVFALLAYAKIGLGLSGIGL
                     WAAGLVHFIMTIWYVLSLLDLSRRSIRI"
     gene            complement(1528..>1730)
                     /locus_tag="VF870_02165"
     CDS             complement(1528..>1730)
                     /locus_tag="VF870_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251012.1"
                     /translation="DPFERAQIDSESYPEWAMDRAFVLVPAQAYVGQFLMTFKDYPQR
                     QEVGSFSLDRVMEKLQESSPKN"
BASE COUNT          508 a          371 c          295 g          556 t
ORIGIN      
        1 aaaggcacat accagaaacg aattgcattt tttgctgatt gaataattga cttgttcata
       61 atgcttaaat ttagttaatt tttattaatt gtttcgttta aaaatttaat ccaaacctga
      121 ttaccgtatc tacattgcat ataaagcgat ctttatgtgc tgatcgcgat aaagtgttcg
      181 aaaatcatct ttgaagaatt ggttcttttg aataaaaaac ctaaggatat tgcattgctc
      241 acgctttaat tatccaactc cccaaaaaaa ggtatgttat ccaacacttc tacacgaatc
      301 gtgctgagaa tatagttttc aatttcatag aactgagccg ccaatttgga gcctgaggct
      361 tttttcacct taccataata ggtgtcaatc agcttatcgc tacttgcttt taaactcttc
      421 acctctttca tgtacgcatc tatttcagcg tcagtgaggt tcatgtaatt ttccgcataa
      481 cgggaaagca gatcaatgcg ctttttacct aattccttgc gcttggcttc gtattcatca
      541 tacacagtcc aaaacggatt ttcgggtgcg agctgtaaaa agccggccac catgtctttt
      601 ttatccatgc caaccgctgc ttgcaacaga tcaacttctt ctgtgtagga ttgggcattg
      661 gcgaatcctg ccattagaat ggctgcaata attaaaatgt tctttttcat aattttaaaa
      721 tttaagattg ataattgtat agatgtaaag tttggtcatg tttcacctgc tccgtggttt
      781 gagccttcag gaatacaacc ggaagtgaaa aaagaaaagt aagtgctatc cgcttcttca
      841 taggagatga aaagtaattt tcaaattcta atagaccttc tacttaaatc caaaagactc
      901 aatacatacc agatggtcat aataaagtgt accagtcctg ccgcccatag gccaatacca
      961 gacaaaccta atcctatttt tgcataggcc aataaagcaa aaacacctgc attataaacg
     1021 gtcaatccaa tgattacacc tttcacagcg ctgctctggt tgtcgttacg tgcgatccag
     1081 caggtaatcc ccaatgccat taatgctact cctgcaatgt tggcgaccat caacgcaacc
     1141 gggctgtcga gcgcagaccc aaataaaaat gaaacaaatt ttgatggaag caaaaccagt
     1201 agcaggccgg ttataaattc aatgactgcg gttaatgtta ataagttctt catgttttac
     1261 ctttttattt gttattgcgt tttttaaatg cattttgtaa tgcagttagg attacttaat
     1321 tcatttattt attcccatcg ccaattctaa atccggctat ttcttaatgt gtcaatgttg
     1381 gctttacccc ctgctgggaa aaaagaggat ctttcgatcc ccttttttcc aaactcccgc
     1441 ttaaagtgtc aatggtggct ttaccccctg tttggcaaaa agaggatctt tcgatcccct
     1501 tttttccaaa ctcccgatta agaaaactta attctttggg ctactttcct gtaacttttc
     1561 catgacccgg tcaaggctaa aactacccac ttcttgtctt tgtggataat ctttaaacgt
     1621 catcagaaat tgaccaacat aggcctgtgc aggaaccaat acaaaagctc ggtccatcgc
     1681 ccactctgga tatgattcag aatcaatctg tgctctttca aatggatcgg
//
LOCUS       DASWBY010000110         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_229503, whole genome shotgun sequence.
ACCESSION   DASWBY010000110 DASWBY010000000
VERSION     DASWBY010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229503"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VF870_02170"
     CDS             complement(<1..608)
                     /locus_tag="VF870_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251013.1"
                     /translation="MKNYLHIILTSVFMLIFFSSVVEAQVTFGTGKINVRVGQYGEIR
                     IFTTEGVDTIQHINRISVIAAGNTDQVIDYWKDLEVNVPTTLIGNPAISDYEISGTYD
                     NTYSLAPPDFLLEQNVYGWSNESYCLGKCTITNRETTQIPILAGLDIVQYVDFTWEND
                     KIFFDATNQVLTQFDIHYVGIKLLSEPMTSAQVFMWYDGYQDS"
     gene            complement(656..>1503)
                     /locus_tag="VF870_02175"
     CDS             complement(656..>1503)
                     /locus_tag="VF870_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEX9251014.1"
                     /translation="QGIAEVPLFSGIGYRIISWAISTTVAILFLLWYVKRLSKNPKVS
                     PTYLQDEERRRNEHFDDVYNQENHFSIRHKAVLITFALCLIMIVVGVIELGWYIEEIS
                     AAFFIMGVVVGIIGGLKIDEFTKAFIEGAKDLVGTALIVALARATLVISRDGQIIDTV
                     LHNLSPYIQSSSPIFASQKMFVVQAIINFFVHSGSGQAALTMPIMAPLSDLAGVSRQT
                     AILAFQFGEYTNIIIPTSAVTMGALSMAKVPWEKWAKWVVPLMVILFVLGFILLIPPN
                     LIGWQ"
BASE COUNT          503 a          298 c          224 g          478 t
ORIGIN      
        1 gaatcctgat aaccatcata ccacataaat acctgggcag atgtcattgg ctcagataac
       61 aatttgattc caacataatg aatatcaaat tgagttagta cctgattagt tgcatcaaag
      121 aatattttat cattttccca agtaaaatcg acgtattgaa caatatctaa tccagctaaa
      181 attggtattt gtgttgtttc tctattagta atagtacatt tgcccaagca ataactttca
      241 ttgctccatc cataaacatt ttgctcgagc aaaaaatcag ggggtgcaag tgaataagta
      301 ttgtcatatg ttcctgaaat ttcataatcg ctaattgcag gattacctat caaagtagtt
      361 ggaacattaa cttctaaatc cttccaatag tctattacct gatctgtatt cccagcagca
      421 attactgaaa ttctattaat atgctgaatt gtatcaacac cttcggtggt gaaaattctt
      481 atctcgccat actgtccgac tctaacatta atttttcctg ttccaaatgt aacttgtgct
      541 tcaactacac tgctgaaaaa aatcagcata aaaacacttg tgagaattat atgtaaataa
      601 tttttcataa cgcacctctt aattaattat tgaatgatta ttaattatca atatattatt
      661 gccatcctat taagtttggc gggattaaaa gaataaatcc cagcacaaat aaaattacca
      721 ttaatggaac aacccatttt gcccactttt cccatggtac ttttgccata gaaagtgcac
      781 ccattgttac agcagaagta ggaattataa tatttgtata ctctccaaat tgaaaagcca
      841 aaattgctgt ttgtctggag actcctgcta aatctgaaag cggagccata ataggcattg
      901 ttaaagctgc ctgtccgctg cctgaatgaa cgaagaaatt aattatagcc tgcacaacaa
      961 acattttttg cgatgcaaaa atcggggaag aggattgaat gtaaggagac agattatgca
     1021 aaaccgtatc aataatttgt ccatctcttg atattactaa cgttgctcta gctaaggcaa
     1081 ctatcaatgc tgtaccaact aaatctttag ctccttcaat aaaagctttt gtgaattcat
     1141 ctattttcag tccgccgatt attccaacaa ctactcccat tataaaaaag gcggcagaaa
     1201 tttcttctat gtaccagcca agttcaatta caccaaccac tatcataatc aggcacaatg
     1261 caaatgtaat caatactgct ttatgtctta tggaaaaatg attttcctga ttgtaaacat
     1321 catcaaagtg ttcgtttctt ctccgctctt catcctgaag atatgttgga cttactttgg
     1381 gattttttga aagccttttc acataccaca ataaaaacaa aatcgctact gttgtagaga
     1441 ttgcccagct aataattcta taaccgatac cagagaataa cggaacctca gcaatgcctt
     1501 gtg
//
LOCUS       DASWBY010000111         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_380007, whole genome shotgun sequence.
ACCESSION   DASWBY010000111 DASWBY010000000
VERSION     DASWBY010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380007"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VF870_02180"
     CDS             complement(<1..782)
                     /locus_tag="VF870_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9251015.1"
                     /translation="MKLPFIIIVDDDEHVLRAIQRDIRNKYHSEYRISATDSANEAIE
                     LIKELKLKNETVAIFISDQRMPEMEGVVFLEKAKEIYPEAKMVLLTAYSDIEVAIRAI
                     NNLKLDYYLLKPWNPPDEKLFPVIDDLLDEWQAQYKPDHEGIRIIGFQWSPKSHRLKE
                     FLSGNLIPYLWMDVETNADAVKYLVSANVKLEGLPLVVLKDGTSLIDPDLPDLAQHIG
                     LRQSASEKMYDVLIIGGGPAGLAASVYGSCEGLKTILIEKSNP"
     gene            complement(800..>1539)
                     /locus_tag="VF870_02185"
     CDS             complement(800..>1539)
                     /locus_tag="VF870_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9251016.1"
                     /translation="SAAQRLKNEDELMYWLEKNHLPVNLQVVETFIDAGFSGADLETL
                     HNSVPKEELAQILLWIENLLSSKRIIKDLGEASARISNLVGAIKSHVHMDRTNELQPT
                     DIHNDIENTLTLLGYKLREKNITVKKVFCENLSNVSAFVGELNQVWTNIIDNAIYALD
                     KEGEITIETTCDSKNINIRIIDNGPGIPSEIQSRIFDPFFTTKKVGEGTGIGLDLVNR
                     IVKHHNGEIKVYSKPGRTEFHVCLPLL"
BASE COUNT          444 a          315 c          254 g          526 t
ORIGIN      
        1 ggattgctct tttcaattag aattgttttc aatccttcac atgaaccata caccgaagca
       61 gctaaccctg caggaccgcc cccgataata agaacatcgt acattttttc ggatgctgat
      121 tgcctcagac ctatatgctg agcaagatcc ggaaggtcgg gatcaatcaa actggttcca
      181 tctttcaaca caactaatgg caatccttca agttttacat tagcactgac taaatatttt
      241 accgcgtcag cattagtttc gacatccatc cataaatatg ggattaaatt tcccgaaagg
      301 aattctttga gacgatgcga tttgggtgac cactgaaaac caattatacg gataccttcg
      361 tggtcaggtt tgtactgtgc ctgccattca tccaataaat catcaattac tggaaaaagt
      421 ttttcatcgg gagggttcca gggtttcagt aaataataat caagctttaa attgttgata
      481 gctcttattg ccacctctat atcggaataa gctgtgagca gaaccatttt agcttcagga
      541 tagatctctt ttgctttttc taaaaataca acgccttcca tttcaggcat acgctggtcg
      601 gaaatgaata tcgcaaccgt ttcgtttttg agttttaatt ctttgataag ttcgatggct
      661 tcatttgctg agtcggtcgc actaattcta tactcactat gatacttatt tcgaatatca
      721 cgttgaattg cacgaagcac atgctcgtca tcatcaacaa ttattataaa tggtaatttc
      781 atcttgttaa ttaattttat tataaaagcg gcagacaaac atgaaattct gttctaccag
      841 gtttcgaata aactttaatt tcaccattgt gatgtttgac tattctgttc acaagatcca
      901 gtcctatccc tgttccttca ccaactttct ttgttgtgaa aaatggatcg aatatcctcg
      961 attgaatttc agacggaata cccggaccgt tatcaatgat tcttatgttt atattttttg
     1021 aatcacatgt tgtttctatt gtaatctcac cttctttatc aagagcataa attgcattgt
     1081 caattatatt cgtccagact tgattcagtt cgccaacaaa tgcagaaacg ttagaaagat
     1141 tttcacaaaa tactttttta actgtaatat ttttttccct cagcttatag cctaacagcg
     1201 ttaaagtatt ttcaatatca ttgtgaatat cagtaggctg taattcattc gtccgatcca
     1261 tatgtacatg gctcttgatg gcacctacca gattagatat ccttgcagaa gcttctccca
     1321 aatctttaat gattcttttt gaacttagaa gattttcaat ccaaagaagg atttgagcaa
     1381 gttcttcctt tggaacacta ttatgaaggg tctcgaggtc tgcaccggag aaccctgcat
     1441 ctataaaggt ttcgactacc tgtaaattaa ccggaagatg gtttttttca agccaataca
     1501 tcagttcatc ttcatttttt aatcgctgtg ctgcactta
//
LOCUS       DASWBY010000112         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_229568, whole genome shotgun sequence.
ACCESSION   DASWBY010000112 DASWBY010000000
VERSION     DASWBY010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229568"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="VF870_02190"
     CDS             <1..1162
                     /locus_tag="VF870_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9251017.1"
                     /translation="THSEGGFYSAEDADSEGEEGKFYLWDVDELRSVLDKKESDFAIK
                     VFNLADDGNWIDESKGMMPGTNILHLTKSNKELANEFNISEDSFSKMSESIRKKLFDY
                     REKRVHPHKDDKILTDWNALMISAFAKAAQALGDNRYAEAAVNSYSFIEKYLIEKDGR
                     LIHRFRDGESGLPAYIDDYAFMINALIDLYETTFDIKYLDRAIELNEILIKEFWDEQN
                     SGFFFSSSTSEKLIARQKDVYDGAVPSGNSVALLNLIRLSRFTANNYFEEKASELTKY
                     FSGYILRSPSAFSMFMCGLDFLFSSSTEIVILCEQKDDLVIDGINVIRRIFNPNKIVI
                     LILNNIDIDFKKLLSFTNDMKMKNNSTTFYVCRDFACNQPVNSIKELEKLLL"
     gene            1248..>2144
                     /locus_tag="VF870_02195"
     CDS             1248..>2144
                     /locus_tag="VF870_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9251018.1"
                     /translation="MSSDNKFVRSLIENAKQGNNAAIEQLFQMNLGKIYAFALRLTAN
                     KSIAESITKETFIEAWKKMNLVRSDASFLKWLSAITVYQTIDSLRSNKNKTKVDHNQL
                     KELESKGELDKHIFELPDQDRMIFVLNKIEGYSIEEISDMMGIKKDQVTVHLGMAISK
                     LASSEPTLSDEAVLKEKIAKVVPELQPSTEVRNGIFSYIMDVKLREQKEQEKIAQALS
                     AEEKQGKDEEEIPIEKEKYVEEEKVKPKKKFKFNPQLFKKLGYTVLVLIAIIVVYIIL
                     TSPSSGWEVISISGQPKVNNSPM"
BASE COUNT          780 a          313 c          402 g          649 t
ORIGIN      
        1 gactcattct gaaggcggat tttattccgc tgaagatgct gatagtgaag gagaagaagg
       61 taaattttat ttgtgggatg tagatgagtt aagaagtgtt ttggataaaa aggaatccga
      121 ttttgctata aaagttttta atcttgcgga tgatggcaac tggattgacg aatcaaaagg
      181 aatgatgcca ggcacgaaca ttttgcactt gactaagtcc aataaagaac ttgctaatga
      241 atttaatata agcgaagata gtttttcaaa aatgtcagaa tcaatccgta aaaaactttt
      301 tgattacagg gagaaaagag ttcatccgca caaagacgac aagattttaa cagactggaa
      361 cgctttgatg atatctgcat ttgcaaaggc tgcacaggct ttgggtgata atagatatgc
      421 tgaggctgca gttaattcat attctttcat tgaaaagtat ctgattgaaa aagacggcag
      481 actaattcat cgtttccgtg atggtgaatc cggtttaccc gcatatattg atgactatgc
      541 ttttatgata aatgctttga tcgatcttta cgaaaccact tttgatatta aatatcttga
      601 ccgtgccatc gaactaaatg aaatattaat taaagaattt tgggatgaac aaaatagcgg
      661 attttttttc agctcatcaa caagtgaaaa acttattgca agacaaaaag atgtttatga
      721 tggtgccgtt ccttcgggaa attctgtggc attgttaaat cttataagat tatcacgatt
      781 tacagcaaac aattattttg aagaaaaggc atccgagctt actaaatatt tttccggata
      841 cattttaaga tcgccgtcag ctttctctat gttcatgtgc ggattagatt ttctattttc
      901 ttcatcaaca gaaattgtaa ttctatgtga acaaaaagat gatctggtta ttgatgggat
      961 taatgttatt agaagaattt ttaacccaaa taaaatcgta atattaattt taaacaatat
     1021 tgatattgat ttcaaaaaac ttttatcgtt tacgaatgat atgaaaatga aaaataatag
     1081 cacgacattc tacgtttgtc gtgattttgc ctgcaatcag cctgtaaact ctataaaaga
     1141 gttagaaaaa ttacttttat aacaactaat tattatcgct taagtagata tttaattata
     1201 gttagactaa ttttgctgta ctaccttaaa taaagctgga gaaacctgtg agttcagata
     1261 ataaatttgt aagatcatta atcgaaaatg ccaaacaagg caacaatgcc gcgatcgaac
     1321 aactatttca gatgaatctt ggaaaaattt atgcgtttgc actgcgttta acagccaata
     1381 aatccatcgc tgaaagcata actaaagaaa catttattga agcatggaaa aagatgaatc
     1441 ttgttagaag tgatgcttca ttccttaaat ggctaagtgc tataactgtt tatcaaacca
     1501 tagattctct ccgctcgaat aagaacaaaa caaaggtaga ccacaatcaa ttaaaagagc
     1561 ttgaatcaaa aggtgaatta gataaacata tttttgaatt acctgatcag gatcgaatga
     1621 tatttgtgct taacaaaatt gaaggttatt cgattgaaga aatttctgat atgatgggaa
     1681 ttaaaaaaga tcaggtaacg gttcatttag ggatggctat ttctaaactc gcaagcagcg
     1741 aaccaacact ttccgatgag gcagtgttga aagaaaaaat tgctaaggtt gtaccggaac
     1801 ttcaaccatc gactgaagta cgcaatggaa tattttcata cataatggat gtaaaacttc
     1861 gcgaacagaa agaacaggaa aaaatagcac aggccttaag cgctgaagaa aagcagggaa
     1921 aagacgaaga agagattcct atagaaaaag agaagtatgt tgaggaagaa aaagtaaaac
     1981 ctaaaaagaa gtttaagttt aatcctcaac tatttaaaaa acttggttac acagttttgg
     2041 tgctgatagc aatcatagtc gtttacatta ttttaacatc accaagcagt ggttgggaag
     2101 taatttctat ctcaggtcag cccaaagtaa ataatagtcc gatg
//
LOCUS       DASWBY010000113         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_117791, whole genome shotgun sequence.
ACCESSION   DASWBY010000113 DASWBY010000000
VERSION     DASWBY010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117791"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1064)
                     /gene="gpmA"
                     /locus_tag="VF870_02200"
     CDS             complement(318..1064)
                     /gene="gpmA"
                     /locus_tag="VF870_02200"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX9251019.1"
                     /translation="MYKVVLLRHGESTWNKENRFTGWTDVDLSEKGLAEAKKAGEVLK
                     KDGYKFDIAYTSVLKRAIRTLWFTLDGLDLMWIPVIRHWRLNERHYGALQGLNKAETA
                     QKFGEDQVKIWRRSYDTPPPALEKSDDRYPGKDPRYADLNENELPLTECLKDTVDRFV
                     PYWLGTIAPMVKSGKRVLITAHGNSLRALVKYLDNIPDNEIVELNIPTGIPLVYELDE
                     NLKPIKHYYLGDQDEIVKAAAAVANQGKVK"
     gene            complement(1169..1516)
                     /gene="mnhG"
                     /locus_tag="VF870_02205"
     CDS             complement(1169..1516)
                     /gene="mnhG"
                     /locus_tag="VF870_02205"
                     /inference="COORDINATES: protein motif:HMM:NF009314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEX9251020.1"
                     /translation="MIEVISGIVILSGSFFILISAIGIIRMPDLFTRMSATTKASTLG
                     VGLVLLGTALFWQDIGISARAIIIITFLFLTAPVAAHIIGRAAYFDKVPLWEKTKVDE
                     LKGKYDEKTHDLK"
BASE COUNT          526 a          310 c          237 g          528 t
ORIGIN      
        1 aaaaccatag atcgtacgag aaactgatgc aaattctacc aaggctaaca attgtcccat
       61 tactacacaa actcctgcag agagtggaag ttcaaatcgt attaatctaa aaagtccttt
      121 taattttttc atttttatta atgattaaaa ataataatag aaaaatttaa caacggagaa
      181 atccaagtca aaccaaatac ggagctttat aagatctaag aaatttattc ttttttaaaa
      241 agaaattatt aaatagtgtt aaaaagaaaa ccctgatttt ttactatcag ggtttttagt
      301 ctaaaagtcg taagtattta tttcactttt ccttgattag caacagccgc cgccgctttt
      361 acaatttcat cctgatcgcc caaataataa tgcttaattg gttttaagtt ttcatccaat
      421 tcataaacta agggaattcc tgttggaata tttaactcaa caatttcatt atcaggtatg
      481 ttatcaagat attttacaag tgcacgtaaa ctgtttccat gtgcagtaat caaaacacgc
      541 ttaccggatt ttaccattgg tgctattgta cctaaccagt atggtacaaa acgatcaaca
      601 gtatctttta aacattcggt aagaggtaat tcattttcat ttaaatcagc ataacgaggg
      661 tctttgccag gatatctatc atcagatttt tctaatgccg gcggcggagt atcataactt
      721 cttctccaaa tctttacctg gtcttcacca aatttttggg cggtttctgc tttgtttaat
      781 ccttgcaaag cgccataatg tctttcattt aatctccagt ggcggataac aggaatccac
      841 attaaatcca atccatcaag agtaaaccat aaagttctaa tcgctctttt aagaacagaa
      901 gtatacgcga tatcaaattt atatccatct ttcttcaaaa cttctccagc tttttttgct
      961 tcagctaaac ctttttcaga aagatcaaca tcagtccagc cggtaaatct attttcttta
     1021 ttccaggtgc tttcgccatg acgtaataaa actactttgt acattttatc tccgattctt
     1081 taaatttatt tatcaaaagt tcatttcaaa aatagggaag cagatttttt agtgaaagtc
     1141 aaattccaag gaaaagggaa taataatgtt atttcagatc gtgagttttt tcgtcgtact
     1201 ttcctttaag ttcatctacc ttcgtttttt cccagagagg aaccttatca aagtaagctg
     1261 ctcttccaat tatatgtgca gcaacaggtg ctgtaagaaa aaggaaggtt attataatta
     1321 ttgctcttgc tgaaattccg atatcctgcc agaataatgc tgtccctaat aaaactagcc
     1381 caacacccaa tgtcgatgct tttgttgttg cagacattcg tgtaaaaaga tccggcatac
     1441 ggattatgcc gattgctgag atcaaaatga aaaacgaacc ggaaagaatt actattccgc
     1501 ttatcacttc aatcatttcc gtgttctcct ttcgaggtaa tacgcaaatg caactgtacc
     1561 aagaaatgct aacaatgcca caataattcc aacatccaaa a
//
LOCUS       DASWBY010000114         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_79484, whole genome shotgun sequence.
ACCESSION   DASWBY010000114 DASWBY010000000
VERSION     DASWBY010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79484"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /gene="upp"
                     /locus_tag="VF870_02210"
     CDS             complement(<1..348)
                     /gene="upp"
                     /locus_tag="VF870_02210"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX9251021.1"
                     /translation="MNKIFFLIEHPIIKRDVTILRDSKTDSETFRAALQRVSNILAVE
                     LSKDFQLTETDVETPLEKTKGAKLTHNVILVPVLRAGLGMVNGFLQIIPEAKVGHIGL
                     QRDEETLKPIEYYY"
     gene            complement(362..1672)
                     /gene="der"
                     /locus_tag="VF870_02215"
     CDS             complement(362..1672)
                     /gene="der"
                     /locus_tag="VF870_02215"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9251022.1"
                     /translation="MKIPLIAIVGRPNVGKSTLFNRLIGKRDAIVDDQSGVTRDRNYG
                     EGDWSGKRFRLIDTGGYVPESSDLFEIAIREQVEVAVAEADSIIFLVDVKNGVNPIDK
                     VIIDLLRQSGKKYFLVVNKVDAQVHAAGAAEFYSLGVDMVYDISALSGRQTGDLLDDI
                     TKDFPTEDIIEDDKRLKIAIVGRPNVGKSSLTNALLGEERSIVTDIPGTTRDSLDSVL
                     KYYGEEIILVDTAGLRKKKKVEESIEFFSNLRTFKAISESDVTVLMLDVQTGFEKQDQ
                     KIIDEVVRWRKGLVIAINKWDVMEKETNTARDYEVAIKEKLGSVDYAPIIFISALTKQ
                     RIYKLIDLCKKVQSERTKKIPTSDLNDAILPEIEKNPPPSSPTGKEVRIKFVNQVGDY
                     YPIFLFFSNYPAHIPDHYRKFLERTIRRIYGFEGVPITVSFRQK"
     gene            complement(1808..>2290)
                     /locus_tag="VF870_02220"
     CDS             complement(1808..>2290)
                     /locus_tag="VF870_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251023.1"
                     /translation="HNWSVVKYAEWLNQYEGASIPINPDQNLPPWERVSWDDVNAAET
                     GSHKLPPYGTQQYYELIGKYHQYSSGWNDFSGGGNKDQISPNFTYYSGMRGDANDFYS
                     VASTAVIGIYVNHFLSALDAVWSATQFNKDLAIKVRLENIQFANRVEFYPKIYFTYSF
                     "
BASE COUNT          701 a          456 c          354 g          779 t
ORIGIN      
        1 gtaataatac tcgataggct ttagtgtttc ttcatctctc tgtaagccaa tgtgcccgac
       61 ttttgcttca ggaattatct gaagaaaacc gttaaccatt cccaatcctg cacgtaaaac
      121 agggactaat attacattgt gggttagttt tgcaccttta gttttttcaa gtggagtttc
      181 aacatcagtt tcggttaatt gaaaatcttt agatagctcc actgctaaga tgttagatac
      241 tctttgtaat gcagcacgaa atgtttctga atcggtttta ctatctctta agattgtaac
      301 atcacgttta attatcgggt gttcaatcag aaaaaaaatt ttattcataa tatttactaa
      361 attatttttg cctgaaactt acggtaatcg gaacgccttc aaatccatat atccttctga
      421 tagttctttc taaaaatttt ctgtaatgat ccggaatatg agcaggataa ttactaaaga
      481 acaaaaaaat cggataataa tcaccaacct gattaacaaa ttttattctt acttcttttc
      541 ctgtcggcga tgatggtggt ggattttttt cgatctccgg taaaatagca tcatttagat
      601 ctgatgttgg aattttcttt gtcctttcac tttgaacttt tttacacaga tcgataagct
      661 tgtaaattct ttgcttagtt aaggcagata taaaaataat cggagcgtaa tcaacagagc
      721 ctaacttttc ttttattgca acttcataat cacgggcagt attggtttct ttctccatca
      781 catcccattt atttattgcg ataactaatc cttttctcca gcgtaccact tcatcaataa
      841 ttttctgatc ctgtttttca aatcctgttt gaacatcgag catcaacaca gtaacatcac
      901 tttcgctaat cgctttaaat gttcttaagt ttgaaaaaaa ctcgatactt tcttctactt
      961 tctttttctt tctcaaaccg gcagtatcaa caagaataat ttcttcacca taatatttta
     1021 ataccgaatc aagactgtca cgtgtagttc cgggaatatc ggtaacaatg cttctttctt
     1081 caccaagtaa cgcatttgtt agtgatgatt taccaacatt aggtcttcca acaatagcga
     1141 tttttaatct tttgtcatct tcaattatat cttcagttgg aaaatctttt gtgatatcat
     1201 caagaaggtc tccggtttgt cttccgctta atgcagaaat atcataaacc atatccacgc
     1261 caagcgagta aaactcagcg gcaccggcag cgtgaacctg tgcatcaact ttattaacta
     1321 ctaaaaaata ttttttgcct gattgccgga gtagatcaat tataacttta tcaattggat
     1381 ttacaccatt cttaacatct accagaaaaa taattgaatc cgcttctgca actgcaactt
     1441 caacttgttc ccggattgca atttcaaata aatcagatga ttcagggaca tatccgccag
     1501 tatcaatcaa acgaaatctt tttccactcc aatcgccttc tccgtagttt ctatcacgtg
     1561 taactccgct ttgatcatca acaatggcat cccgcttgcc tataagtctg ttaaaaagcg
     1621 ttgatttgcc tacgttaggt cttcctacaa ttgctattaa tggtattttc atatctgaaa
     1681 aataaggaat tatggtgtgg gaatgaaaga tattaccagc attccataga aaacctgcct
     1741 tgtaatgcaa gaaatctcgg ggaaatgaat gaactaaaat tataaacttt cagacgatag
     1801 gttttaacta aaaactataa gtaaaataga tttttggata gaactctacc ctatttgcaa
     1861 actggatatt ttcgagtctt actttaatag caagatcttt attgaactga gtcgcagacc
     1921 agacagcatc caatgcactt aaaaagtgat taacataaat acctattacg gcagttgatg
     1981 caactgaata aaaatcattg gcatcaccgc gcataccgga ataatatgta aaattaggtg
     2041 aaatttgatc tttattaccg ccgccgctaa aatcgttcca gccggatgaa tattgatgat
     2101 atttcccaat cagctcataa tactgttgtg taccataggg tggtaattta tgagaaccgg
     2161 tttctgcagc atttacatca tcccaactta ccctttccca cggcggcagg ttttggtcag
     2221 gattaatagg tatgcttgca ccttcatatt gatttaacca ttctgcatat ttaacaacac
     2281 tccagttatg
//
LOCUS       DASWBY010000115         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_267193, whole genome shotgun sequence.
ACCESSION   DASWBY010000115 DASWBY010000000
VERSION     DASWBY010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267193"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1518)
                     /gene="hslU"
                     /locus_tag="VF870_02225"
     CDS             complement(118..1518)
                     /gene="hslU"
                     /locus_tag="VF870_02225"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX9251024.1"
                     /translation="MEKKPFDMKMFTPSQIVKELDKYIIGQTEAKRAVAIAIRNRWRR
                     QQVKDILREEIMPNNIILIGPTGVGKTEIARRLAKLSQAPFVKVEASKFTEVGYVGRD
                     VESMVRDLVEVGVNLVKAEKAVEVQAKAEKMADERVLDLLIPPVKKTQTTPAVQETEE
                     QPANNEENEAYQNQKTREWMKEKLKNGEMDDRMIEFDSQAQSSIGMQVLGPFGMDDMG
                     INIQEIMGNIMPKKRKKRKTTVKEARQILAQEEMQKLIDMDVVQKEAIERIENSGMIF
                     IDEIDKVAGGGKGQGPDVSREGVQRDLLPIVEGSNVNTKYGVVRTDHILFIASGAFHV
                     SKPSDLIPELQGRFPIRVELNSLTEDDFVQILTTPQNALLKQYGALLETEGVKLSFSK
                     DGIKEIARIATEVNEQVENIGARRLHTILTTLLDEILFDVPDKIPSDTVKIDAKFVKD
                     KLEKIVKDRDLSKFIL"
     gene            complement(1521..>1936)
                     /gene="hslV"
                     /locus_tag="VF870_02230"
     CDS             complement(1521..>1936)
                     /gene="hslV"
                     /locus_tag="VF870_02230"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX9251025.1"
                     /translation="KIANGKVLCGFAGASADAFTLMERFEDKLEQYRGNVNRAAVELA
                     KDWRTDKYLRKLEAMLAVISKDTALVISGTGDVIEPDDQIVAIGSGGMYALAAAQMLK
                     KHSKLSSKEIVHEALKTAADICIYTNDKINVEVIE"
BASE COUNT          536 a          419 c          302 g          679 t
ORIGIN      
        1 gtcattgcga atcccaacgg gatgaagcaa tctgcaaact ggtaagagct ataaattttg
       61 attcgagatc acttcgtccc gaataatcgg gactcgtgat gacattctaa agatactcta
      121 taaaataaac ttacttaaat ctctgtcttt aacaattttt tcaagtttat cttttacaaa
      181 ttttgcatca atttttactg tgtcggatgg aattttatcg ggtacatcaa aaagtatttc
      241 atcaagtaaa gtggtaagga ttgtgtgcag tcttcttgca ccaatgtttt caacctgttc
      301 attaacttca gttgcaatgc gggcaatttc ttttatgccg tctttgctaa aacttaattt
      361 cactccttct gtttcaagaa gcgcaccgta ttgttttaac aatgcatttt gcggagtagt
      421 taatatctga acaaaatcat cttcggttaa actgttaagt tcaacacgaa ttggaaatct
      481 tccctgcagt tccggaatta aatccgatgg tttagaaaca tgaaaagctc cggatgcaat
      541 aaacaaaatg tgatcagttc ttacaactcc gtatttagta tttacatttg agccttcaac
      601 aattggaaga agatcacgct gtacaccttc acgactaaca tcaggtccct gtcctttacc
      661 accgccggca actttatcaa tttcatcaat aaatatcatt cctgaatttt caatacgctc
      721 aatagcttct ttctgaacaa catccatatc aataagcttt tgcatttctt cctgagcaag
      781 aatttgtctg gcttctttta cagtagtctt tcttttcttt ctcttcttcg gcattatgtt
      841 gcccataatt tcctggatat taattcccat atcatccata ccaaatggtc caagcacctg
      901 cattccgatt gatgattgtg cctgagaatc aaattcaatc atcctgtcat ccatctcgcc
      961 gttttttaat ttttctttca tccactcgcg ggttttttgg ttttgatagg cttcgttctc
     1021 ttcattattt gcaggctgtt cttcagtctc ctgaacagcg ggagtggttt gtgttttttt
     1081 aaccggaggg attaataaat caagtactct ttcatctgcc atcttttcgg ctttagcttg
     1141 aacctcaaca gctttttctg ctttaacaag attaacgccc acttcaacaa gatcacgcac
     1201 cattgattcg acatctcttc caacataacc aacttctgta aattttgatg cttcaacttt
     1261 tacaaatgga gcttgtgaaa gctttgctaa tctgcgtgca atttctgttt taccaacacc
     1321 ggtaggacca ataaggataa tattattcgg cataatttct tcgcgaagaa tatctttaac
     1381 ctgctgtctt ctccagcgat tacgaattgc aattgcaaca gcacgttttg cttctgtctg
     1441 gccaattata tatttatcaa gttccttaac gatctgagaa ggagtaaaca ttttcatatc
     1501 aaaaggtttc ttttccataa ctattctata acctctacat taattttatc atttgtgtat
     1561 atgcaaatat cagctgcggt tttaagtgct tcatgtacaa tttctttaga agaaagtttg
     1621 ctgtgcttct ttaacatttg tgcagcagca agagcataca ttccgcctga tccaatagca
     1681 acaatttgat catccggttc aatcacatct cctgtacctg atattacaag tgctgtatct
     1741 ttagaaataa ccgcaagcat tgcttccagc tttcttaaat atttatctgt gcgccaatct
     1801 tttgcaagct ctacagcggc acgattaaca ttgccgcgat attgttcaag tttatcttca
     1861 aatctttcca taagtgtaaa tgcatcagcg gaagcaccag caaaaccgca aagcactttt
     1921 ccgttagcaa tttttc
//
LOCUS       DASWBY010000116         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_456157, whole genome shotgun sequence.
ACCESSION   DASWBY010000116 DASWBY010000000
VERSION     DASWBY010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456157"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2090)
                     /locus_tag="VF870_02235"
     CDS             complement(<1..2090)
                     /locus_tag="VF870_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX9251026.1"
                     /translation="MKNILISLLIIFSLVPFSDISSQGHEKGINLIPQPKSIVINEGT
                     FNLNAATKIYYQKEAKQIAEYLNEIISPSTGFNFSLSEWNNKVDGNSIIISLSNSSRD
                     LGKEGYTLVVNSNNVMIEAAELNGLFYGVQTLRQLLPPEIESKTKVENVNWEIPSVVI
                     LDEPEFSWRGLNLDCCRHFMTKEFVKRYIDLLAYHKFNTLHWHLTEDQAWRIEIKKYP
                     ELTKKGAFRTYDDGSVYGGFYTQDDIKEIVKYAASRFINVVPEIEMPGHSTAAISCYP
                     EISCTGGPFEVGTLWGIYYDIYCAGNEKTFQFLEDVISEVVELFPYKYIHVGGDEAPK
                     NRWKECPKCQKRIKDEGLADEHELQSYFIKRMEKFINSKGKQIIGWDEILEGGLAPEA
                     TVQSWRGVKGAIEAAKQGHDVIVSPTSHCYLDYPVDITDMQKVYLFDPVPPELTADER
                     KHVLGSEGNMWSEYAPQELIDDRLFPRMLALSEVVWTYPEERNFDEFSQRVQEHYNRL
                     EVLGVNYGLETKPFEIIKSFNTETNAFDLQITQLQKDLDLYVFSKQRGPIMSNPIGVE
                     RFDIQVPQTEELEFSFLRNQTSVGKIFRHSFTFNKATRKNVELTYPFSEKYSAGGVAA
                     LTDGMRGSDSFRGGDKSWQGYQGTDFEGKIDLGEVTDINKISIGFFQASSSWVVFPKY
                     VEFFTSSDGIEYQSA"
     gene            complement(2117..3088)
                     /locus_tag="VF870_02240"
     CDS             complement(2117..3088)
                     /locus_tag="VF870_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9251027.1"
                     /translation="MKYRKLGKSDVEVSEISLGCWTMGGLNWVNGTPNGWANVDEKEI
                     SQAINYAIDHGVNHFDNADVYGNGRAERMLARILDKRTNNFIIATKIGWFPGTAEHAY
                     EPKHIRHQCEQSLINLKRDHIDLYYFHHGDFGENDKYLDDAIDVMYKLRDEGKIRLIG
                     QSAYTHEDFVKLIPKVKPDVIQSFASALDDRFVQDNSPTRKMLDQYKISFVAFGPIAQ
                     GLLLGKYHQGNPPQFDAGDHRANAERFKPENLAKLEPKIEKLKNKFGPSNQELSRAAL
                     QYLLYYNQVGSVIPGFRNLDQVKSNLSGMDNPLTEEEFNFIKKVFAE"
BASE COUNT         1049 a          661 c          524 g         1160 t
ORIGIN      
        1 gcactctgat attctatacc atcagatgat gtaaagaatt caacatactt tggaaaaaca
       61 acccacgatg atgaagcctg aaaaaatcca atagaaattt tatttatatc cgtaacctct
      121 ccaaggtcaa tctttccttc aaaatcagtt ccctggtaac cttgccagga tttatcacct
      181 ccacgaaatg aatccgaacc acgcattcca tcagttaatg cggcaactcc acctgcagaa
      241 tatttttcac taaacggata agttaattcg acattttttc tggtcgcttt gttaaaagta
      301 aaactatgtc tgaatatttt tccaacacta gtttgattcc gcagaaaact aaactctaat
      361 tcttcagttt gaggaacctg aatatcaaat ctttccactc caattggatt agacattatc
      421 ggaccgcgct gtttgctaaa cacataaagg tccaaatcct tttgaagctg agtaatttgc
      481 aaatcaaagg cattcgtttc tgtgttaaat gattttatta tttcaaaagg ttttgtttcc
      541 aatccataat taactcccag tacttccaat ctgttataat gttcttgaac tctctgactg
      601 aactcatcaa aatttctttc ttccggatag gtccaaacga cttccgataa tgctaacatt
      661 cgtggaaaca atctgtcatc gattaattcc tggggagcat attcgctcca catatttcct
      721 tcacttccaa gcacatgttt tctttcatct gcagttaatt caggtggaac aggatcaaac
      781 agataaactt tctgcatatc agttatatca acaggataat ccagataaca atggcttgtt
      841 ggagaaacaa ttacatcgtg cccttgttta gcagcctcaa tcgcgccttt aactccacgc
      901 catgattgaa cagttgcttc gggtgcaagt cctccttcaa gaatttcatc ccagccaata
      961 atttgttttc cttttgagtt gatgaatttt tccattcgct tgataaaata actttgtaat
     1021 tcatgctcat cagctaagcc ttcatcctta attcttttct gacactttgg acattccttc
     1081 catctatttt taggtgcctc atctcctcca acatggatat acttataagg aaatagttca
     1141 actacttcgc tgataacatc ttctaaaaac tgaaaagttt tttcatttcc cgcacaataa
     1201 atgtcgtaat aaattcccca aagcgtgcca acttcaaatg gaccgccagt gcaagaaatt
     1261 tcgggatagc aggaaattgc agccgtagaa tgcccgggca tttcaatttc gggaacgaca
     1321 tttataaatc tgcttgcagc atacttaaca atttctttaa tatcatcctg agtataaaat
     1381 ccgccataaa ccgaaccatc atcataagtt ctgaatgcac ctttttttgt aagttctgga
     1441 tattttttta tttcaattcg ccaagcctga tcttcagtaa gatgccagtg aagtgtatta
     1501 aacttgtggt aggcaagaag atcaatatat ctttttacaa attccttagt cataaaatga
     1561 cggcagcagt caaggtttaa tcctcgccag gaaaattcag gctcatcaag aattacaaca
     1621 gaaggaattt cccaattaac attctcaact tttgtttttg attctatttc aggtggaaga
     1681 agctggcgaa gtgtttgaac accataaaat aatccgttta actcagcagc ttcaatcata
     1741 acattgtttg aattaaccac cagtgtataa ccttctttac ctaaatcacg tgaagagttt
     1801 gagagagaaa ttatgattga attgccatca actttattat tccactcact caaactgaaa
     1861 ttaaatcctg ttgaaggact gattatttca tttaagtatt ccgcaatttg ctttgcttct
     1921 ttttgataat agatttttgt ggcagcgttt aagttaaaag taccttcatt aataacgata
     1981 cttttaggtt gtggtattaa gtttatcccc ttttcatgac cctgtgatga aatatcacta
     2041 aagggaacta atgaaaagat aatcagcaga gaaatgagaa tatttttcat agtatttttt
     2101 tatttgtgat aaaatattat tcggcaaaaa cttttttaat aaaattaaat tcttcttcag
     2161 ttaatggatt atccattccg ctcaaatttg atttaacctg atcaagattt ctaaaacctg
     2221 gaattaccga acctacctga ttgtaatata aaagatattg caatgcggct cttgaaagct
     2281 cctgatttga cggaccgaat ttatttttaa gtttttcaat ttttggttca agctttgcaa
     2341 gattttcagg tttaaatctc tctgcatttg ctctatgatc accagcatca aattgcggcg
     2401 ggttgccctg gtgatattta ccaagtaata acccttgtgc gatagggcca aacgcaacga
     2461 atgaaatttt atactgatca agcattttac gtgttggcga attatcctgt acaaaccggt
     2521 catcaagtgc acttgcaaaa ctttgtatta catcaggttt tacttttggt atgagtttaa
     2581 caaaatcttc atgcgtataa gcagattgac caatcaatct tatcttacct tcatcacgaa
     2641 gtttgtacat aacatctatt gcatcatcaa gatatttatc attctcccca aaatccccgt
     2701 gatgaaaata atacagatcg atatgatctc ttttaagatt tataagtgat tgttcgcatt
     2761 gatgtcggat atgtttgggt tcgtaagcat gctcagctgt tccagggaac cagccaattt
     2821 ttgtagctat tataaaattg tttgttcgtt tgtcaagaat tcgggccagc attctttctg
     2881 ctctgccatt tccataaaca tcagcattat caaaatggtt aactccgtga tcaatggcat
     2941 aattgatggc ttgggatatt tctttttcat ccacattagc ccaaccgttt ggcgttccgt
     3001 taacccaatt aagtccgccc atagtccagc agccaagact aatttctgat acttctacat
     3061 cggatttacc aagttttctg tatttcattt tatttccctc ttaaatgtat ttgtaaaaat
     3121 ataaaagaat tggaggatat tgaactctaa ttaacaataa ttaatagttt aagattttgg
     3181 atagatttga attagtgctt tgggaagtaa aaattaaata atataacatg aaaattattg
     3241 gcaggctaaa aagctttatt cttaacctgc cgttaggaga gataacacat tttatttaac
     3301 taaaattaaa aatctataag atattctgtg ccatgcagct tgtgataact gtagtataag
     3361 tttttgcaaa ttttactaac tctttcatta tcat
//
LOCUS       DASWBY010000117         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_154676, whole genome shotgun sequence.
ACCESSION   DASWBY010000117 DASWBY010000000
VERSION     DASWBY010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154676"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..376)
                     /locus_tag="VF870_02245"
     CDS             complement(143..376)
                     /locus_tag="VF870_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX9251028.1"
                     /translation="MKEFIEFIVKNIVDKPDNISVEENTPNDHTIEFKLKVDESDLGK
                     VIGKQGKNVNAIRTLITAVGAKTHHRITLQLIE"
     gene            complement(478..1038)
                     /locus_tag="VF870_02250"
     CDS             complement(478..1038)
                     /locus_tag="VF870_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251029.1"
                     /translation="MKYTYAVISMLLFFQSLNHSQNYDYWKDAPVEGTLIFSIYFVNS
                     QVGYAMSTNTEIFKTSDSGLTWKIEQNNANAKLPDEKNIYWSAEIYCSAMKTEDGGNS
                     WTPYSEKAQEHFCKVYFKDPNVEYKIASEFLYTISEKVSSLLKTNKIETLIGRPQQCT
                     EYYSNEEEGWAVGWCLKNFNQYKNTK"
     gene            complement(1121..>2229)
                     /locus_tag="VF870_02255"
     CDS             complement(1121..>2229)
                     /locus_tag="VF870_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251030.1"
                     /translation="DSLIAWGIQLESSQNTVIENNNVQNLKSTVNGSDIVQPGINSYN
                     GFGDTIRNNIVHNLRANAGYTTVGILLSGWAGMIGSNNLVYNNMIYDINSTSTSYDSR
                     VTGIQMWHQNDPKIYYNSVFLSGIGSNKYGSAALYVYLGTANADIRNNVFVNTRDESP
                     YCASAIRAYSTYSTFTSDYNDLYYEFSPYNCLVTTGNNDYHTLVDWQVTGKDLNSVNE
                     MPNFISPDLHIFAGPSYLESNATPMLEITEDFDGNPRHAITPDIGADEFNGVAVGVKY
                     EEMLPTQFALLQNYPNPFNPSTKISWQVPVGGLQTLKLFNVLGNEIETLFNDYKPAGK
                     YEVQFNASTLSSGVYFYQLKAGNYIEIKKMILIK"
BASE COUNT          693 a          404 c          344 g          788 t
ORIGIN      
        1 taatgagcaa aaacaatttg aaaaagaact tctgcatagg aacctcctaa taatttgatg
       61 attaaataat cagggtgttc agtcttgagt gttaacaata tgattgaaca ctctaaatgc
      121 ttaaagtaaa agacaaatac aatcattcaa ttagttgaag ggttattctg tggtgcgttt
      181 tagcaccaac tgcagtaatt aatgttcgga ttgcatttac atttttaccc tgtttcccga
      241 taactttgcc aagatcgctt tcatctactt taagtttgaa ttcaattgtg tgatcgtttg
      301 gtgtgttttc ttctacgctt atgttatccg gtttatcaac aatatttttt actatgaatt
      361 caataaattc tttcattttt atatcctttc aatataagtg tactgtaata ctgtgttttg
      421 gaaataagat ctcagccaat aaaatccata gtaacctcct gttatatttc ataaaaatta
      481 ttttgtgttt ttatactgat tgaaattttt caagcaccat ccgactgccc agccctcctc
      541 ttcattgctg taatactccg tacactgttg cggtcttccg attagtgttt caatcttatt
      601 ggtttttaat aatgagctga ctttctctga aattgtataa aggaattctg aagcgatttt
      661 atattcaaca tttggatctt tgaagtaaac tttacaaaag tgctcctgtg ctttttctga
      721 gtaaggagtc cagctgtttc caccatcttc cgtcttcatt gctgagcagt aaatttcagc
      781 tgaccaataa atatttttct catcaggtaa ttttgcattt gcattattct gttcgatctt
      841 ccaagtaaga ccagaatcag acgttttaaa aatttcggta tttgtcgaca tcgcgtatcc
      901 cacctgtgaa ttgacgaagt atattgaaaa aatcaatgtg ccttctacag gagcatcctt
      961 ccagtagtca taattttgag aatgatttaa cgattgaaaa aataggagca ttgaaataac
     1021 tgcatatgta tatttcattt tataactcct caaaatcttc taaatactta tgagaaatca
     1081 tttgcttact tatcttctaa ccaggataag ttcgataaat ttattttata agtatcattt
     1141 tctttatttc gatataatta ccagctttta actgatagaa atatactccg ctggatagag
     1201 tagaagcgtt aaactgaact tcatacttac cagctggttt gtaatcatta aatagagttt
     1261 caatttcatt tcccaaaaca ttaaacaact ttagtgtctg caagccgcct accggtactt
     1321 gccaactgat ttttgtgctt gggttaaatg ggtttggata gttttgcaac agtgcaaatt
     1381 gtgttggaag catctcttca tatttaacgc ctactgcaac tccattaaac tcatctgccc
     1441 ctatatccgg tgttatagcg tggcggggat ttccatcaaa atcttcagtg atttccagca
     1501 ttggtgttgc attcgattca agataagaag gaccagcaaa tatatgaaga tcaggactaa
     1561 taaagtttgg catctcgttt acactattta gatcttttcc agttacctgc caatctacaa
     1621 gcgtgtgata atcattgttt ccagttgtta ctaagcaatt ataaggactg aattcataat
     1681 acaggtcatt ataatccgat gtgaaagtag aatatgttga atacgcacga attgcagaag
     1741 cacagtaagg agattcatct ctcgtattca caaatacatt atttctaata tctgcattag
     1801 cagtacccaa ataaacatac aaagcagcag aaccatattt attagatcct ataccactca
     1861 agaaaacaga gttataataa attttgggat cattctgatg ccacatctga atacccgtaa
     1921 ctctgctatc atatgatgtt gaagtactat taatatcata gatcatatta ttataaacta
     1981 gattattact gccaatcata cctgcccacc ctgataataa tattccgaca gttgtatatc
     2041 cagcattggc tctaagattg tgcactatat tattacgtat agtatcacca aaaccattat
     2101 aagaatttat tccaggttgt actatatcac taccattcac agttgatttt aagttttgaa
     2161 cattattatt ctcaatgaca gtgttttggg aactttctaa ttgaataccc caggcaatta
     2221 agctatcgg
//
LOCUS       DASWBY010000118         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_118171, whole genome shotgun sequence.
ACCESSION   DASWBY010000118 DASWBY010000000
VERSION     DASWBY010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118171"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VF870_02260"
     CDS             complement(<1..855)
                     /locus_tag="VF870_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9251031.1"
                     /translation="MVALQAVLNPQEEVLFPAPYWVSYPDMARMIGAIPVAALPEDGT
                     FYPNIKDIIDRVGSYTKAIIINSPNNPTGAFYSEDFIAEVVEFCEKKDIWLIMDDIYH
                     RLLFDGKKPINCFKYAKKKDENSKLIVINGVSKQYAMTGFRIGWAVGNKKVIEAMSNI
                     QGHQTSGPSVLLQKAAVAAINGIQSGVESLRATLENNRNVMVDLLRSFEGVKVSKPDG
                     TFYCLADFSAYEKDSNKLSEFLIDKVQVLTVPGKEFGLDGHLRLSYCGSIKDVQDGIE
                     RMKWALDPN"
     gene            complement(865..1164)
                     /locus_tag="VF870_02265"
     CDS             complement(865..1164)
                     /locus_tag="VF870_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251032.1"
                     /translation="MSLSLIAKSIKASPTLKLNEKFAILKEKGDPVIHLGGGEPKSKV
                     PMDAILATVVHVNTGEVRYAPADGIPALKQAVIRYTEEFYERKVRPENVIASGGA"
BASE COUNT          489 a          308 c          252 g          524 t
ORIGIN      
        1 gtttggatca agagcccatt tcattctctc aattccatcc tgaacatctt ttattgatcc
       61 gcaatagctt aatcttaaat gtccgtctaa tccaaattcc ttacctggaa ctgttaatac
      121 ctgaacttta tcaattaaaa attccgataa cttgtttgaa tctttttcat aagcagaaaa
      181 atctgcaaga cagtaaaatg ttccatcagg tttagaaact tttacacctt caaacgatct
      241 tagcaaatca accataacat ttctgttatt ttctaaagtt gcacgtaaac tttccacacc
      301 ggattgaatt ccgttgattg cagcaacagc agctttttgt aaaagtacag aaggacctga
      361 agtctgatgt ccctgaatgt tagacatcgc ctcaattact tttttatttc caacagccca
      421 gccgattcta aatccagtca tggcatactg tttagaaact ccgttaataa caataagttt
      481 agaattttca tctttctttt ttgcatactt aaaacagtta ataggtttct ttccatcaaa
      541 caacaagcga tgataaatat catccataat taaccagata tctttcttct cacagaattc
      601 aacgacctct gcaataaaat cctcactgta aaaagcgccg gtaggattat tcgggctgtt
      661 gattataatt gcttttgtat aagagccgac tctgtctata atatctttaa tgttcggata
      721 aaaagttcca tcttcaggaa gtgccgcaac tggaattgcc ccgatcattc ttgccatatc
      781 tggataactt acccaatatg gagcggggaa aagaacttct tcctgcggat ttaatactgc
      841 ctgaagcgct accataattg cctgctatgc accgcctgat gcaataacgt tttccggtct
      901 tactttcctt tcataaaact cttcagtgta tctgatgaca gcttgtttaa gtgcaggaat
      961 accatctgca ggcgcataac gcacttctcc agtgttaacg tgtacaacgg ttgcaagaat
     1021 tgcatccatc ggtaccttac tttttggttc gccgccgcca agatgaatta cagggtcacc
     1081 tttttctttt aagattgcaa acttttcatt tagctttaaa gtgggcgagg ctttaataga
     1141 ttttgcgata agactgagac tcatttagaa cctctgattg tgtgaataat aattattaaa
     1201 attaacactt ctaaattatt cgtgatggcc tagagttaca agaattttaa tatctttttc
     1261 aaaaatggtc acaaaaaatt aagatattta tttatcaagc ggaatatatt actatcaact
     1321 aagagcaggg gattatgttg aaactaagaa gatgattctt ttgaaataaa aaagtcacga
     1381 atttgtttgt gactttcttc tctgttatac taaaaacttt ttcttcaaag catcatgtac
     1441 atttggaggt acaaaactgc taacatctgc atgaagagat gcaagatttc taatcacagt
     1501 agagttaagg taagtatatc tttcgtgagg cattaaaaat attgtagtaa tatcaccgga
     1561 cagttttctg ttc
//
LOCUS       DASWBY010000119         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_718812, whole genome shotgun sequence.
ACCESSION   DASWBY010000119 DASWBY010000000
VERSION     DASWBY010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718812"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VF870_02270"
     CDS             complement(<1..275)
                     /locus_tag="VF870_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251033.1"
                     /translation="MKTFICVITFIFLALPLLAQQKDLTGIWTGKLALPNSMELTIVF
                     NLSQNDSGIYSSTLDSPDQGAKGIPTESTTLNGDSILIKIPVVQGFYA"
     gene            complement(397..609)
                     /locus_tag="VF870_02275"
     CDS             complement(397..609)
                     /locus_tag="VF870_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX9251034.1"
                     /translation="MTNTQKILIFLGVVIIIIGFTWPWLSKIPFGKLPGDIVVDKPNF
                     KFFFPITSMIIVSLVISFIIWLVKKF"
     gene            complement(606..1001)
                     /locus_tag="VF870_02280"
     CDS             complement(606..1001)
                     /locus_tag="VF870_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9251035.1"
                     /translation="MNGAGIILLNSNNQVLLLLRDDKVDIPFPGMWDIPGGKVEKNES
                     PEDAVRREMNEELGLKDLGEIELFRIFTSENLTDYVFWKRLDLNPEEIDLKEGQKIEY
                     FSQDQIKETKLAFNYNEVLEEFFSELLKR"
     gene            1150..>1909
                     /locus_tag="VF870_02285"
     CDS             1150..>1909
                     /locus_tag="VF870_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9251036.1"
                     /translation="MKNQNSKTAIIIGSGLGGLSTALRLSTEGYDVTLIEKHSKPGGR
                     LNQLKLDGFTFDVGPSFMSMSWELEELFKSNGLKNPIQLKELDPLYQVYFEGENKPYR
                     IWKTLNNLEEEFKDIEPNLAAKVEKYLARAGEFFHDTEDKVVKSNYKNVADYILKLTR
                     VPKKHLPYLFRNMWSEVEKNFESEQVRVIFSLVAFFLGSTPFHTPAIYSLLNYTEMKH
                     DGYWKVKGGMYKIVEELMKILEARGVKFVFNTEIV"
BASE COUNT          667 a          303 c          311 g          628 t
ORIGIN      
        1 gcataaaacc cctgtacaac aggaattttt ataagaatag aatctccatt taatgttgta
       61 ctttcagtag gtatgccttt agccccttga tcaggactat ccagggttga tgaataaatt
      121 ccagaatcat tttgtgataa attaaataca atagttagtt ccattgaatt cggtaaagca
      181 agtttacctg tccatattcc agttaaatct ttttgttgag ctaataaagg aagagcaaga
      241 aaaataaacg tgataacgca gataaaggtt ttcatattaa ctccatatcg ataaaaatat
      301 tttttgctaa ccaaaattaa gtaataaaga atttctttta cagattatca gccagatgga
      361 aataaataca aatacacggt tattttattg tttcaatcaa aattttttta cgagccagat
      421 tataaacgag ataaccagac tgactataat catagaagta ataggaaaga aaaatttaaa
      481 gttaggttta tcaactacaa tatcgccagg taattttcca aaaggtattt tagataacca
      541 cggccaggta aatccgatta ttattattac aacgcctaaa aagattaata ttttttgtgt
      601 gttagtcatc gtttcagtaa ctctgaaaaa aactcttcaa gaacttcatt gtagttaaat
      661 gctaatttag tttctttaat ctgatcctga ctgaaatatt ctattttttg tccttccttc
      721 aaatcgattt cctccgggtt cagatctaat cttttccaga aaacataatc agtaagattt
      781 tccgatgtaa aaatcctgaa cagctcaatt tcacccaaat ctttaagccc caattcttca
      841 ttcatttccc ttctcacagc atcctcaggt gattcatttt tttcaacttt tccaccagga
      901 atatcccaca tgccagggaa agggatgtca actttatcat cacgcaataa tagtaaaact
      961 tgattattgc tgttaagaag gattattccg gctccgttca agaatccaat tccttagttt
     1021 gtgtatcata cgaaataaga ttattataag atattaaaat tctactctcc agggcgaaaa
     1081 tctattattt aagtaaaaat attcctgctt ttaggaacta aatataaatt atttccgttt
     1141 gatatatata tgaaaaatca aaattcaaaa acagcgatta taataggaag cggacttggt
     1201 ggattatcta cggctttacg tttatctaca gaaggttacg atgtaacatt aattgagaaa
     1261 cactcaaaac caggcggcag attaaatcag ctaaagctgg atggatttac ttttgatgtt
     1321 ggtccttctt ttatgagtat gagctgggaa cttgaagagt tatttaaaag taacgggtta
     1381 aaaaatccaa ttcaacttaa agaacttgat ccgctttatc aggtttattt tgagggtgaa
     1441 aataaaccat acagaatctg gaaaacttta aacaatcttg aggaagaatt taaagatatc
     1501 gaaccaaatc ttgctgcaaa agttgaaaag tatttagcac gcgcaggaga gtttttccac
     1561 gataccgaag acaaagttgt taaatcaaat tataaaaatg ttgctgatta tattttgaag
     1621 ttgacgcgtg ttccaaagaa acatcttcca tacttattta gaaatatgtg gagtgaagta
     1681 gaaaagaatt ttgaatcaga acaggttaga gtgatatttt ctttggttgc atttttcctg
     1741 ggttcaactc catttcatac tcctgctata tactctttac ttaactatac agaaatgaag
     1801 cacgatggct actggaaagt taaaggcgga atgtacaaaa ttgttgaaga gttgatgaag
     1861 attctagagg ctcgcggagt aaaatttgtt tttaacactg agattgttg
//
LOCUS       DASWBY010000120         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_229893, whole genome shotgun sequence.
ACCESSION   DASWBY010000120 DASWBY010000000
VERSION     DASWBY010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229893"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VF870_02290"
     CDS             <1..839
                     /locus_tag="VF870_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEX9251037.1"
                     /translation="KGFKLKHFDPSDTLDLKSEDKPKAQSALRDGIQLLSDLQDKLYA
                     QDKWAVLLIFQAMDAAGKDSAIKHVMSGINPQGCQVFSFKSPTSEDLDHDFMWRCLKR
                     LPERGRIGIFNRSYYEETLVVKVHKEILDKQKIPENLITKHIWDERYQDISNFEKYIN
                     RNGILVRKFFLYVSKEEQKKRFLERIDNPEKNWKFSSADVAERGHWDEYMKAYEDMIR
                     NTATKDSPWYVVPADNKWFTRLVVAAAVIDSLNSLELNYPEVGEEKLKELAGVKTKLV
                     SE"
     gene            883..1968
                     /gene="cax"
                     /locus_tag="VF870_02295"
     CDS             883..1968
                     /gene="cax"
                     /locus_tag="VF870_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX9251038.1"
                     /translation="MKNFFRPGLNWLLLFIPISFVLEAAHSSEPLIFFSAALSIIPIA
                     KLIVHSTEQLSTYTGDAIGGLLNATFGNFPELIIAVVALKAGLYTMVLASIVGAILSN
                     LLLALGLSFFIGGTKFHTQEYNPISARVYASMMFMAVISLAVPSSFNSLFGTQDVFKQ
                     ESLLNIGLAIVLLLGYGLYLFFMIKTHPEFFKSNKDEAGEQHAEPWSKGLSILMLVIA
                     SVGAAFMSEVLVGAAEGTGQALGMSATFIGIVFLAVVGGAAESFSAIAMAQKNKMDLS
                     VGILLGSCIQITLFVAPVLVLLSLFVGTEPLNLSFPRSQIGALFFAVIIGSLISGDGK
                     SNWFKGVQLIAIYIIIALMFFFIPDAI"
     gene            1979..>2577
                     /locus_tag="VF870_02300"
     CDS             1979..>2577
                     /locus_tag="VF870_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251039.1"
                     /translation="MSEINFWKKIQDEAGSILDPISRISEVLFGLIMVLSFTGSISVL
                     SDGKAEIKDLLWAALGCNLAWGIIDAVFYLMSRVFEKGHGYSVLRKLKSTNDKAEGRK
                     LITNELPPVISAILKPEEVDQLNDRIVKLDDAPKTAIISLTDLRAAFMILLLVFTCTF
                     PVALPFIFINNTALALRLSNGIALLILFFGGFSVGKFAG"
BASE COUNT          787 a          417 c          529 g          844 t
ORIGIN      
        1 gtaaaggatt taaactcaag cactttgatc catctgatac attggactta aaatccgagg
       61 ataaacccaa agctcaatca gcattaagag atggtattca actgctatct gatttacaag
      121 acaaacttta cgctcaggat aaatgggcgg tgcttctgat ttttcaggca atggatgctg
      181 caggaaaaga tagtgcgatt aaacatgtaa tgtctggaat taatccgcaa ggatgccagg
      241 ttttttcttt taaatcacca accagtgaag atcttgatca cgattttatg tggagatgtt
      301 tgaagcgatt gccggagcgc ggaagaattg gaatttttaa tagaagttat tacgaagaaa
      361 ctctggttgt aaaagtacac aaagaaattc ttgataaaca aaaaattcct gaaaatctta
      421 taactaaaca catttgggat gaacgttatc aggatattag taattttgaa aaatatatta
      481 acaggaatgg aattttagtt agaaaatttt tcctctacgt ttcaaaggaa gagcagaaaa
      541 aaagatttct cgaaagaata gataatcctg aaaagaactg gaagttttct tccgctgatg
      601 tagcagaacg tggtcattgg gatgagtata tgaaagcata tgaggatatg atccgaaata
      661 cggctacaaa agattcaccc tggtatgtgg ttcctgcaga taataaatgg tttacacgac
      721 tggttgtcgc ggcagcggta atagattctc ttaactcact ggaattaaat tatcctgaag
      781 ttggtgaaga aaaattaaaa gaacttgctg gagtaaaaac aaagcttgta agcgagtagt
      841 tcaggtaaat ttattttttt ataaagtaga aggtaaaccc aaatgaaaaa ttttttcaga
      901 ccaggactca attggctgct actctttatc ccaatatcgt ttgtactcga agctgctcac
      961 tcttcggagc cattaatttt cttttcagca gctttatcaa ttataccaat tgctaaactt
     1021 attgttcatt ctacagaaca gttatcaacc tatacaggtg atgctattgg aggattgctg
     1081 aatgcgacat ttggtaactt ccctgaatta ataattgcag ttgtagcatt aaaagctggt
     1141 ctttatacga tggtattagc ttcaatagtt ggagcaatac tttcaaatct tctgcttgct
     1201 ttaggtttgt cattttttat tggcggtaca aaatttcaca ctcaggaata taatccaatt
     1261 agcgcacggg tttatgcttc tatgatgttt atggctgtaa taagtttggc tgttcccagc
     1321 tcgtttaatt cactcttcgg tacacaggat gtttttaaac aagaaagttt actgaatatt
     1381 ggattagcaa ttgtgcttct tcttggatat ggcctttatc ttttcttcat gataaaaaca
     1441 caccccgagt tttttaaaag taataaagat gaggcaggtg agcaacatgc agagccttgg
     1501 tcaaaaggtc tctccattct tatgcttgtt attgcatctg tcggagcagc atttatgagt
     1561 gaggtactcg ttggagctgc agaagggaca ggtcaagctc ttggtatgtc agctaccttt
     1621 ataggaattg tgtttcttgc agtggttggc ggtgcggctg aaagcttttc agctatagct
     1681 atggcacaga aaaataaaat ggacttgagt gttggaattt tattaggaag ctgcattcaa
     1741 ataactttat tcgttgcacc tgttctggtt ttattaagtc tttttgtggg aactgaacca
     1801 ctgaatcttt catttccacg atcacaaatt ggagctttgt tctttgcggt aataattggt
     1861 tctttgatct ccggagacgg caaatcaaat tggttcaaag gagttcaatt aattgcaatt
     1921 tatataataa ttgctttgat gttcttcttt atacctgatg cgatttaagg aaaattgtat
     1981 gtcagaaata aatttttgga agaaaatcca ggacgaagca ggaagcattt tagatccaat
     2041 cagcaggatt tctgaagtac tttttggatt aattatggtg ctttcgttta ccggctccat
     2101 tagtgttctt tcggatggca aagcagaaat taaagatcta ttatgggcag ctttgggatg
     2161 caatctcgcc tggggaatta ttgatgctgt tttttatttg atgagcagag tgtttgaaaa
     2221 aggtcacggg tattcagttt tgaggaaact caaatcaaca aatgataaag ctgaaggcag
     2281 aaagttgatt acgaatgaac tacccccagt catatcggca atattaaaac ccgaggaagt
     2341 tgatcagctt aacgatagaa tagttaaact tgacgacgca cccaaaacag cgataatttc
     2401 cttgacggat ttacgagctg ctttcatgat tttactgctt gtatttacat gtactttccc
     2461 ggttgcactt ccatttatat tcataaataa tactgcatta gctttacggc tttcaaatgg
     2521 aatcgcactg ctcattctat tctttggtgg attttcagtt ggtaaatttg ctggctt
//
LOCUS       DASWBY010000121         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_718861, whole genome shotgun sequence.
ACCESSION   DASWBY010000121 DASWBY010000000
VERSION     DASWBY010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718861"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VF870_02305"
     CDS             complement(<1..1340)
                     /locus_tag="VF870_02305"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX9251040.1"
                     /translation="MKNIIINSKLDHPILIVISMLIVISFAFIPSKPDEGMFPLSDIK
                     NINLNEKGLKISVDEVYNPNGVSLVDALVKIGGCTGSFVSNEGLIITNHHCVFGIVSN
                     LSTVENNYLENGFLADTRVKELPAKGLTCRITESYEDVSAEVLEAANTAEDISQRTTA
                     ISKKIKEIVEREEKNDSTIKAEVSEMFVGEQYVLFRYKTINDVRLVYVPPRTIGEFGG
                     ESDNWVWPRHNGDFSFVRAYVAPNGSSAKYSEKNIPYQPKKFLQVNPNGVNEEDFVFI
                     LGYPGRTFKHQPSQFLMMQEKYQLPYIQDLYSWLINLYEERGKDDPEFALEIVGQIQG
                     LANTEKNYRGKMQGLERLKLIQKKQEEEKELQAYINSDPQLKEKYGTVLSEIDEVYKD
                     AFNSGRRSLVLMMLSRNVDYYRLAEIFIDYKKEMQKPESERKSLYKEDKKADFY"
BASE COUNT          454 a          309 c          220 g          550 t
ORIGIN      
        1 tcataaaaat cagctttctt atcctcttta tataatgatt ttctttctga ttcaggtttc
       61 tgcatttctt ttttataatc aataaagatc tctgctaaac ggtaataatc cacatttcgg
      121 ctaagcatca ttaatacaag acttcttcta ccactattaa atgcatcttt ataaacttca
      181 tctatttcgc ttaatactgt tccgtatttt tcctttaact gcggatcgga atttatatat
      241 gcctgaagtt ctttctcttc ttcctgtttt ttttggatta acttcaatct ttctaggcct
      301 tgcattttgc cgcgataatt tttttctgtg ttggctaatc cctgaatttg accgactatt
      361 tctaatgcaa attccggatc gtctttacct cgttcttcat aaagatttat taaccaggaa
      421 tataaatcct gtatatatgg taattgatat ttttcctgca tcattaagaa ttgtgagggc
      481 tgatgtttga aagttctgcc cggataaccg agtatgaaaa caaaatcttc ttcatttact
      541 ccgtttggat ttacctgcaa aaactttttc ggctgataag gaatattctt ctcagagtat
      601 tttgcagacg aaccattggg tgccacataa gcacgtacga aagaaaaatc accgttgtgt
      661 cttggccaca cccagttatc agattcaccg ccaaactcac caattgtcct aggaggcacg
      721 taaaccaatc ttacatcatt tatagttttg tatctgaaca gaacgtactg ctcaccaaca
      781 aacatttcag aaacttcggc ttttattgtg ctatcattct tttcttctcg ttcaacaatt
      841 tcttttatct ttttagatat tgctgtagtg cgctgagaaa tatcttctgc agtgtttgca
      901 gcttctaaaa cttctgcact aacatcctca tatgattctg taattctgca ggttaatcct
      961 ttggccggta attcttttac gcgtgtgtca gctaaaaacc cattttcaag ataattattt
     1021 tcaacagtgc tcaggtttga aactattcca aaaacgcaat gatgatttgt tataattaat
     1081 ccttcgttgg atacaaatga accagtgcac ccacctatct ttacgagcgc atcaacaagg
     1141 ctaactccat ttggattata aacttcatca acacttattt ttaatccttt ttcatttaag
     1201 ttgatgtttt taatatcact taacggaaac attccctcat ctggtttgga tggaatgaat
     1261 gcgaatgaga ttacgattag cattgaaatt acgattagaa ttggatgatc aagctttgaa
     1321 tttataatga tgtttttcat ttgtattcct taaatattta actgtccaaa tataaaaaaa
     1381 caggctcggt ttcccgagcc tgaaaagaag tccatccaaa ccttccctaa aggaaggttt
     1441 agaaaaaatt aaataatgat tacaacaaag attctttcaa tcttctagca actgcaacaa
     1501 agaaaccaag caccattaca attacaccta tcc
//
LOCUS       DASWBY010000122         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_192505, whole genome shotgun sequence.
ACCESSION   DASWBY010000122 DASWBY010000000
VERSION     DASWBY010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192505"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VF870_02310"
     CDS             <1..660
                     /locus_tag="VF870_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX9251041.1"
                     /translation="TFIDEDELRICDDTTALIFDSMKPGAYSSREFLWEFGKYLDENG
                     GPKVDDSVVYAAYKKNVPIFVPAFSDCSAGFGFVMHQHKNPDNHVSCDSGKDFLELTK
                     IKLHSKDTGIFMIGGGVPKNFTQDIVVAADILQEDAEMHKYAIQITVADERDGALSGS
                     TLKEASSWGKVSTSSEQMVYAEATVAMPLIVGYAYHKGGWKNRKAREFQKIYSKENAI
                     A"
     gene            937..1437
                     /locus_tag="VF870_02315"
     CDS             937..1437
                     /locus_tag="VF870_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX9251042.1"
                     /translation="MRIIIAGMGDVGYQLAKQLSNESHDIIAIDMDQQRLSYSDQMAD
                     ILTIKGSSTSIKVLNEAGVSKADLLVAVTSSEEVNIATSIIAKKLGANKTIARISNAE
                     YLDPTIKINFAEIGVDFMIYPEELAAIETVSLINRTAATDVLEFEDGKLAVIGLRLDK
                     NAHIAN"
BASE COUNT          608 a          265 c          344 g          538 t
ORIGIN      
        1 acgtttattg atgaagatga actaagaatt tgtgatgata ccactgcgct aatttttgac
       61 agcatgaaac ccggagcata ttcatcaaga gaatttttgt gggagtttgg gaaatatctt
      121 gatgaaaatg gcggaccaaa agttgatgat tctgttgttt atgctgcata caaaaagaac
      181 gtcccaattt ttgttcctgc attttcagat tgttcagcag gattcggatt cgttatgcat
      241 caacataaaa atccggataa tcatgtttcg tgtgattccg gaaaagattt tctggagtta
      301 acaaaaataa aattacacag caaagatact ggcatattta tgatcggtgg tggtgttcct
      361 aaaaacttta ctcaggacat tgttgtggct gcagacattc tacaggagga tgctgaaatg
      421 cacaagtatg caatacaaat tacagttgca gatgaaagag atggtgcact ttcaggctca
      481 actttaaaag aggcaagttc ctggggtaaa gtatctacat cttctgagca aatggtatat
      541 gctgaagcaa ccgttgctat gcctttaatt gttggatacg cttatcataa aggtggatgg
      601 aaaaatagaa aagccagaga gtttcaaaag atttattcca aagagaatgc aattgcataa
      661 aaactaaaaa cgcctcaaat gaggcgtttt taatttaatc caattactct taggtctccc
      721 ccagggacct ggtagtagaa taaccctggg atcaatttta catagcaaat ctaaatttta
      781 gttatactgt aataaatagt taatttgcac tattttagtc atgtaaaacg cgattttaat
      841 gagtttttaa ttgacatcga acaattattt gtactgagta aaatatttta tacttttatt
      901 tataatcttt gaacaccaaa tactattgaa tttgatatga gaattattat tgccggaatg
      961 ggagatgttg ggtatcagtt agcaaaacag ctttccaatg aatcacacga tattattgca
     1021 attgatatgg atcagcagcg tctttcttat tccgaccaga tggctgacat acttacaatt
     1081 aaaggttctt ccacttcgat aaaagtttta aatgaagccg gagtttcaaa agctgatctg
     1141 cttgttgctg ttacttcatc tgaagaagta aatattgcta catcaataat tgcaaaaaaa
     1201 cttggagcaa ataaaacaat cgcaaggatt tcaaatgcag aatatttaga tccaaccata
     1261 aaaataaatt tcgctgaaat tggtgtggat tttatgatct acccggaaga attggctgcg
     1321 attgaaacag taagcttaat aaatagaacc gctgctactg atgttttaga atttgaagat
     1381 ggaaaacttg cggttatcgg gttaaggctt gataaaaacg cacacattgc aaattagaat
     1441 cttgcagaga ttgcgaatga atttaaaaac acagatttca gagtcttagc tattcatagg
     1501 aacttcaaaa ctattatacc aaaaggaaat gataggtttc ttgctaacga tcaagtattt
     1561 gtaattacta aaccggaggg acacgaacaa attctaaaac ttgccggtaa ggaaaatctt
     1621 aattttgata atattatggt gcttggcgga ggcaagatag gcagaagagt tgctagtttg
     1681 ctaagtgata aaatgcaggt aaaattaatt gaatcagatc ctgaaaaagg gtttgaactt
     1741 gcagatcaat tacca
//
LOCUS       DASWBY010000123        10704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_493485, whole genome shotgun sequence.
ACCESSION   DASWBY010000123 DASWBY010000000
VERSION     DASWBY010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 10704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10704
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493485"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VF870_02320"
     CDS             <1..1158
                     /locus_tag="VF870_02320"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX9251043.1"
                     /translation="NMTLSTNAQTVKPFWWTIIGSDQGFLNAPLSQRELLIAPGERYD
                     VVIDFSDPALLGQTIIVRNNAKKPFPNGVAVNPNTTGQIMAFRVGTTPVVDPVVLPAT
                     LRQSPVAALTPSNTRKLMLFEGMDEFGRMQPMLGTIDPTNPDPFLDGTLMWNDPTTEF
                     PLLGSTEVWEIYNATVDAHPMHLHLVQFQILGRQKFTATVTAKDNMAHDGSMSMGGKL
                     SNIKLKGKLTKPGLYESGWKDTGIMYPGEVTSVIAKFEREGEYVWHCHILSHEDHEMM
                     RRFEVVPALLPKNGEEETIAAINGFELEQNYPNPFNPSTTIKFSLPKNSFVSLKVFNS
                     LGQEVGTLINQHIPEGNHRVEFNAGDLPSGMYLYTLATNDYKETKKMLLMK"
     gene            1350..3182
                     /gene="ftcD"
                     /locus_tag="VF870_02325"
     CDS             1350..3182
                     /gene="ftcD"
                     /locus_tag="VF870_02325"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX9251044.1"
                     /translation="MNEQLIECVPNFSEGQRPEIIKQITDEIEKVEGVKLLDVDPGYD
                     MNRTVVTFIGNAKGVKEAAFSSIKKAAELIDMSKHKGSHPRMGATDVCPFVPVNGITT
                     EECIELSKEVAKRVGEELGIPVYLYEKSAAKLERENLAKIRQGEYEALEEKLKNPEWK
                     PDYGPTKFNAKAGGTVIGVREFLIAYNINLNTRESKYATDIAFELREKGRSAREGGDG
                     PFYFKSENILKYEKDKYPCGSCDFTGKKIDETIKHCKEVHNYDLIELLDLNGIDYKRP
                     EGQSVKIPGKFKHCKAIGWMVEKYDRAQISINLTNYKVTSMHHVFDETEKLAAERGLR
                     VTGSEIVGMVPFPALLETGKHYLRKQHRSTGVPVKDILNTAVQSLGLNDVSKFKIEER
                     VLGLPKNLDTALVEMKLTDFVDEVSRETPAPGGGSIAALAGALGAALSSMVANLTANK
                     RGSDPDTDKILNEAANKCQEIKNTLVKAVDDDTNAFNDYMAARRLPNKTPEEKKARET
                     AMQNGLKQAVRVPLNTAQLSYQAIEISQTVAKYGNPNSITDVGVGAQSAYTGVLGGIY
                     NVLINLKDIKDEKFNSEMRKTCAELKDKAQKKLKEVLEIVEGKL"
     gene            complement(3366..5558)
                     /locus_tag="VF870_02330"
     CDS             complement(3366..5558)
                     /locus_tag="VF870_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX9251045.1"
                     /translation="MKSLVQYILSVLIIATNLFADGFIVIPRPNPLPNPFPLEVIYHK
                     VDVKIDGQTAVTKIDQSFYNPSQYQLEGFYIFPIPKGAVVNNFTIIINGEETKAELLD
                     ADKARKIYEDIVRQMRDPALLEYSEQNIFKLRIFPIEPRSEKKISISYSQVLESDNNL
                     FEYIYPLNTEKFSARPLKNVSVKIDLKSDEKIKNIYCPTHEIDIVDKSDNHSIISYEA
                     ENVKPDKDFKLYFSKNFSSVGLSLLTYKSGKNDGYFLLSASPSIEIDKSNIEKKDITF
                     ILDVSGSMSGEKLDQAKKALLYCINNLNSGDQFNIIRFSTEAYSLFNSLKSADKQNIN
                     EAKRFIDELQAIGGTNIEEAFNLAFNNYKASSRPHFIIFLTDGKPTIGEMNDEKLIKR
                     VLNMNKNNSRIFTFGIGNEINTHLLDKLTDATKAWRTYVSADEDIEIKVSNFYDKIQS
                     PVLSDLKLDFDNVEVYQTYPKDLPDLFKGSNILVFGRYKGKANTKITLSGKLNGKQKG
                     FTLQGNFTSDDDKYNFIPPLWASRRIGYLLDLIRLNGESKELVDEVTSLAREHGIITP
                     YTSYLIMEDEEFRLRGGRLVDGLQTLPPRPELKKENEADYFRMKEKSGSGSIRVSEEF
                     QSLNSAINYNQTQQGNERLSYTDSEGNKRNLSQQVKNVMGRAVYQQDKFWVDSELQER
                     QTKNVKRIQFNSDEYFALLNKEPETAQFLALGQNVRFYYKDVFYEVYE"
     gene            complement(5657..6781)
                     /locus_tag="VF870_02335"
     CDS             complement(5657..6781)
                     /locus_tag="VF870_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX9251046.1"
                     /translation="MKSLFILTFVFIYSIFSYSNQMVDKKPAKNSSIQLALLLDTSGS
                     MDGLIDQAKSQLWKIVNELATSKKNGRPVNLNVALYEYGKQSIPADEGYLRNIVPFTG
                     DLDKISDELFKLRTNGGDEYCGKVILNAVENLQWNKSNDNLKIIFIAGNEPFTQGDID
                     YKTACQNAIKKGIVVNTIFCGNYDEGVQTMWKDGADLADGKYMNIDHNAAIIHIDAPQ
                     DAEIIRLGQELNKTYIAFGSAGREKKEMQAEQDANSMSLSPGVMVERSVTKSGGQYKN
                     SGWDLVDAKKDGSVKIEDLKDEDLPVEMKEMTVQERKSYVDKMEKDREQIQNQINKLN
                     DERSKYIAQKMIQNKNENTLDAVMIKTIREQAKQKNYKFE"
     gene            complement(6866..7609)
                     /locus_tag="VF870_02340"
     CDS             complement(6866..7609)
                     /locus_tag="VF870_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX9251047.1"
                     /translation="MKKLFLIISITLALTHPNFNQEIPDGFVEIREVIPDIILDLRYT
                     TNHNFLGVPVQGYISQKCYITKAAADSLSKVQAELRHFNLSLKIYDAYRPQQAVDHFV
                     RWAKDLSDTLTKKEFYPTVDKSRLFIDGYIAEKSGHSRGSTVDLTVVPIPLPQQPEFD
                     IDNQCECFESFDKRFKDNSIDMGTGFDCFHKLSHTENPKLTPQQRANRLLLKSLMDKY
                     GFKNLSEEWWHFTLRNEPYPKTYFNFIVE"
     gene            complement(7627..9123)
                     /locus_tag="VF870_02345"
     CDS             complement(7627..9123)
                     /locus_tag="VF870_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX9251048.1"
                     /translation="MKKIFPPYVYNPITLIGAAIAALSSGLIIFLFILDFFSTEENTY
                     MGILTFIVIPTFLIIGLLLIAYGIFRERRRERKGLIRGKYLPVIDLNDPKKRAMFLTF
                     SIGTIVLMLFSAFGSFKAYEYTETDEFCGTICHKVMEPEYTAYLDSPHSRVGCVGCHI
                     GSGTNWYVKSKISGAYQVYSVLFNKYSRPIPTPVEALRPAIGTCEQCHTPALFYNEKK
                     VDYSYYLSDEQNTKSNISMLIKIGGGKSELGSIEGIHWHANKDNVIKYIYTDERRLVI
                     PWVKVKSKNGKETIFRDKNSNFDEKKFNKDNFRTMDCIDCHNRPSHIYHQPDKMMNEM
                     LSKNRVDETIPYIKSISVEVLEDKYSTTQQALESIEQRITDFYNSNYPEIYNSKREAI
                     KTSIDVVKKIYKRNYFTYMNANWKHFPDNIAHTYTPGCFRCHDGNHVSDDGKVISNDC
                     NSCHKIISQTDNTGNTRVDLNGLKFQHPVNLGESIENHLCTDCHWKQE"
     gene            complement(9158..10057)
                     /locus_tag="VF870_02350"
     CDS             complement(9158..10057)
                     /locus_tag="VF870_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251049.1"
                     /translation="MKIKMKTENITTANNLEIDNETVTQEKQVLRFTRLNRGLHFFMI
                     LSFLNLALSGMTLKFSYTGWARFLSSLYGGFESAGYLHRFSALVMVTVFIIHIVDLIK
                     NKRKEAGSFKALIIGPRSMMFNKKDLNDFIGSIKWFLGKGERPEYGRWTYWEKFDYFA
                     VFWGMIVIGSTGFILWFPEFFTLFIPGFMINVATIIHSDEALLATGFIFTVHFFNTHL
                     RPEKFPMDVCIFTGRVPLEEFKLDRPKEYEEIVRNGVLNKYLIEAYPPIVLKAIKLFG
                     WTVLIIGFSMIIWIIYAMIFAYR"
     gene            complement(10035..>10704)
                     /locus_tag="VF870_02355"
     CDS             complement(10035..>10704)
                     /locus_tag="VF870_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251050.1"
                     /translation="VTATCTNCHTAHRELPPDDPMSSVNQANIAKTCGNCHHGIEEKF
                     EKSIHSPLINKTDKKLPVCSTCHSAHSITRADAEGFKLTIMSQCGQCHEKISETYFDT
                     YHGKVSQLGYTKTAKCYDCHGAHDILGVNDPKSRLSRKNIVETCKSCHPSANRQFAGY
                     FTHATHHDPEKYPVLFWTFWGMTGLLLGTFILAGLHTLLWLPKSLQWRRELKKKHSEN
                     KNED"
BASE COUNT         3384 a         1863 c         1844 g         3613 t
ORIGIN      
        1 aatatgactt tgagcaccaa tgctcaaaca gttaaacctt tctggtggac aattattggt
       61 tcagatcagg gatttttgaa tgcgcctcta tcacagcgtg aacttcttat tgcaccgggt
      121 gaacgttatg atgtggttat agatttttct gatcctgcat tacttggtca aacaattatt
      181 gtacgtaata atgcaaaaaa acctttccca aacggtgttg cagttaatcc aaatactaca
      241 gggcaaatta tggctttcag ggttggaact acacctgttg tagatccggt tgtattacca
      301 gcaacattaa gacagagtcc tgttgcagca ttaacaccga gtaacactcg caaattaatg
      361 ttatttgaag gaatggatga gtttggcagg atgcagccta tgcttggtac aattgatcca
      421 actaatccag atccttttct tgatggaact ttgatgtgga acgatccaac tactgaattt
      481 cctctactag gttcaaccga agtttgggaa atctacaatg caacggtaga tgcacatcca
      541 atgcacttgc atttggttca gttccagata ttaggaagac agaaatttac tgcaacagta
      601 acagccaaag ataatatggc acacgatgga tcaatgtcaa tgggcggcaa gctttcaaat
      661 attaaattga aaggaaagtt gaccaaacct ggattgtatg aatctggctg gaaagatact
      721 ggaataatgt atcctggaga agtgacaagc gttattgcca aatttgaaag agaaggtgag
      781 tatgtatggc attgccacat tctttctcat gaagatcatg aaatgatgag aagatttgaa
      841 gtggttcctg cattattacc taaaaatgga gaagaagaaa ccattgctgc aattaacgga
      901 tttgaattgg aacaaaatta tccgaatccg tttaatccaa gtactacaat taaattttct
      961 cttcccaaaa attcgttcgt tagtttgaaa gtttttaaca gtcttggtca ggaagttggt
     1021 actttaataa atcaacacat tcctgaaggc aatcataggg tagaatttaa tgccggagat
     1081 ctgccgagcg gaatgtatct atatacttta gctacaaatg attataaaga aacaaagaaa
     1141 atgttactta tgaagtaatt ctcttaaaaa taggtatata tcaaaggcga ttctctgaat
     1201 cgcctttttt aatttaaatg aaaatttaga tgagattggg ctctatttgt tttaattttg
     1261 taacaatatt ttttgtgaat agtgtcatac tgagcggagt cgaagtataa ccctttcaac
     1321 aattcgatca aaatttattt gaggatcaaa tgaacgaaca acttattgaa tgcgtaccaa
     1381 acttctcaga aggtcagcga cctgaaatta ttaaacagat aacagatgaa attgaaaaag
     1441 tagaaggtgt aaaactttta gatgttgatc ccggttatga tatgaaccgt acggttgtaa
     1501 cttttatagg aaatgccaaa ggtgtaaaag aagcagcatt tagttcaatt aaaaaagctg
     1561 cagagcttat tgatatgagt aagcacaaag gttcgcatcc aagaatgggc gctacagatg
     1621 tttgtccttt tgttcctgta aatggcatta caaccgaaga atgtatcgag ctttcaaaag
     1681 aagttgcaaa aagagttggt gaagaacttg gaattccggt ttatctctat gaaaaatctg
     1741 cagctaaact tgagagagaa aatcttgcaa agataagaca gggtgagtat gaagcacttg
     1801 aagaaaagtt aaagaatccc gaatggaaac cagattatgg tccaactaag ttcaatgcaa
     1861 aagcaggcgg aaccgtgatt ggtgtgaggg aatttcttat cgcttataat atcaatctga
     1921 atacaagaga atccaaatac gcaactgata tcgcttttga gttaagagaa aagggaagaa
     1981 gcgcacgtga aggcggcgat ggtccctttt attttaaaag tgaaaatatt ttaaagtatg
     2041 aaaaagataa atatccttgc ggcagctgtg attttaccgg aaagaaaatt gatgagacta
     2101 tcaaacattg taaagaggtt cataactatg atttaattga gcttctggat ttgaatggta
     2161 ttgattataa aagaccagaa ggacaatcag ttaagattcc cggcaaattt aagcattgta
     2221 aagctattgg ctggatggtt gaaaaatatg atcgtgcaca gatttcaatc aatcttacaa
     2281 attataaagt tacatcaatg catcacgtat ttgatgaaac agaaaagctt gctgctgaaa
     2341 gaggattgcg agtcacaggt agtgaaatag tcgggatggt tcctttcccc gctttgcttg
     2401 aaacaggaaa acattattta agaaagcaac atcgctcaac aggagtgcca gttaaagata
     2461 ttttaaacac agcagttcaa tctttgggtt tgaacgatgt ttcgaaattt aagattgagg
     2521 agagagtttt aggtttacca aaaaatcttg atactgcact tgttgaaatg aagcttacag
     2581 attttgttga tgaagtatca agagaaactc ctgctccggg cggaggttcc atcgctgcac
     2641 ttgcaggagc gctcggcgca gctttatcat caatggttgc aaacttaaca gcaaacaaac
     2701 gcggtagtga tcctgatact gataaaattc ttaatgaagc tgctaataaa tgccaggaaa
     2761 taaaaaacac tttagtaaaa gctgtggatg atgatacaaa tgcttttaat gattatatgg
     2821 ctgcacgcag attaccaaat aaaactccgg aagaaaagaa agcccgagaa acagcaatgc
     2881 agaatggatt gaagcaggca gttcgtgttc ctttaaatac tgctcaatta agttaccagg
     2941 caattgaaat ttcacagacg gttgcaaagt atgggaatcc aaattctatt acggatgttg
     3001 gggttggagc acaaagcgct tacacgggtg ttctcggcgg aatttataat gtgcttataa
     3061 atcttaaaga tattaaagat gaaaagttta attctgaaat gcgaaaaacc tgcgcggagt
     3121 taaaagataa agcccagaaa aaacttaaag aagttttaga gattgttgag ggaaagttgt
     3181 aataatttta acaaacacag tttctttgtg ggtcagttgc ataacagcga aatggtcatt
     3241 tcgaaggagg cttcgactga gaaatctttt gatcatgtta cgaaaaagat ttctcccttc
     3301 ggtagaaata acaattattt agcttgcaca acagaccctt tgtggaactg ggtttgtggt
     3361 ttaaattact catatacctc ataaaataca tctttataat aaaatcgtac gttttgtcct
     3421 aatgctaaaa actgtgctgt ttccggttct ttatttaaca aagcaaaata ttcatcagaa
     3481 ttaaactgta tccgttttac gtttttcgtt tggcgttcct gcaactcact atcaacccaa
     3541 aacttatcct gctgataaac tgctctgccc ataacatttt taacctgctg agaaagattt
     3601 ctcttattgc cttcagaatc tgtatatgat aaccgttcat taccttgctg agtctgatta
     3661 taattaattg ctgagttcag gctctgaaat tcttcactta ctcttatgct gccgctgccg
     3721 cttttctctt tcattctaaa ataatccgct tcattttctt tttttaattc tggtctgggt
     3781 ggaagagttt gcaaaccatc aacaagtcga cctcccctta atctaaactc ttcatcctcc
     3841 attattaaat aacttgtata tggagtaata attccgtgct cacgtgcgag tgatgtaact
     3901 tcatcaacca gttccttact ttctccattc aaccttatca aatcaagtaa gtaacctatt
     3961 cttcttgaag cccataaagg aggaataaaa ttatatttat catcatcaga agtaaaattg
     4021 ccttgtaaag taaatccttt ttgcttgccg tttaattttc ctgacaaggt gatctttgta
     4081 tttgcttttc ctttgtatct tccaaagacc agaatgttgg atcctttaaa caaatccggg
     4141 agatcttttg gatatgtttg atacacctct acattatcaa aatctaattt taaatcacta
     4201 agaacaggcg actgaatttt atcataaaag tttgatactt taatttcaat atcttcatca
     4261 gcgcttacgt aagtccgcca tgcttttgtt gcatcagtca gtttatcgag aagatgagta
     4321 tttatttcat tgccgattcc aaaagtaaat atcctagaat tgtttttatt catattcaaa
     4381 actcgtttaa tcaatttctc atcattcatc tcaccaattg ttggtttgcc atccgttaaa
     4441 aaaataataa aatgaggacg agaagatgct ttgtaattat taaaagccag gttaaatgct
     4501 tcctctatgt tcgtcccgcc gatcgcctga agttcatcta taaatctttt tgcttcattg
     4561 atgttttgtt tatctgctga tttcaatgag ttgaacaatg aatatgcttc agtagaaaaa
     4621 cgaatgatgt tgaactgatc gccgctattt aaattattaa tacaatatag taatgctttt
     4681 tttgcttgat ccaatttttc tccgctcata ctgccggaaa catcaagaat aaaagtaatg
     4741 tctttttttt caatattact tttatcaatc tcaattgatg gtgaagcgct caataaaaaa
     4801 tagccatcat ttttgccgga cttataagtt aaaagagata atccaactga agaaaaattt
     4861 tttgaaaagt aaagtttaaa gtctttatct ggtttaacat tttcagcttc atacgaaatg
     4921 attgaatgat tatcactctt atcgactata tcaatttcgt gagttggaca gtaaatgttt
     4981 tttatttttt catcagactt caaatcaatc tttacagaaa catttttaag tggccttgca
     5041 gaaaattttt ctgtgttaag tggatagatg tattcgaaca aattattatc agattcaagt
     5101 acttgtgaat agctgattga tattttcttt tcacttcttg gttcgatagg aaagatgcga
     5161 agcttaaaaa tattttgttc actatactct aacaaagcgg gatcgcgcat ttgccttacg
     5221 atatcttcgt aaatttttct tgctttatcg gcatctaata actctgcttt tgtttcttca
     5281 ccatttatta taattgtaaa gttgtttact actgctccct ttggaattgg gaatatataa
     5341 aacccttcaa gctgatactg acttggatta taaaatgatt gatcgatttt tgtgactgca
     5401 gtttgcccgt caatctttac atccactttg tgatatataa cttctaaggg aaatggattt
     5461 ggtaatggat tagggcgggg aattactata aatccatcag caaataaatt tgttgcgata
     5521 attaaaacac tcaagatata ttgaactaaa cttttcatac gattctcata ttaaaatatt
     5581 tattttctta gcggctttgc gtctctgcgt gaacttttat cttatttctc gcaaagactc
     5641 taagtcgcaa agtgatttat tcaaacttat agttcttttg ttttgcttgt tcacgaattg
     5701 tcttaatcat aacagcatcg agtgtgtttt catttttatt ttgtatcatt ttttgagcta
     5761 tgtacttgct acgttcatca tttaatttat ttatctgatt ctggatttgt tctctatctt
     5821 tttccatctt atccacataa gactttcttt cctgaactgt catctctttc atttcaacgg
     5881 gaagatcttc atcctttaag tcttcaatct tgactgagcc gtctttcttc gcatctacaa
     5941 gatcccaacc ggaattttta tactggccac cggatttggt gacagatctt tccaccataa
     6001 ctcccggact cagactcata gaattagcat cctgctcagc ctgcatttct ttcttttctc
     6061 tgccggcaga gccgaatgcg atataagttt tatttaattc ctgtccaagc cttattattt
     6121 ctgcatcttg tggtgcatca atatgaatga tagctgcatt atgatcaatg ttcatatatt
     6181 ttccatctgc taaatcagcc ccatctttcc acatcgtctg aacaccttca tcataattcc
     6241 cacagaaaat tgtgtttaca acaatccctt ttttgattgc attctggcat gcggttttat
     6301 aatcaatgtc accttgagta aatggttcat tcccagcaat aaaaattatt tttaagttat
     6361 catttgattt attccattgc agattttcta ctgcatttaa tattactttg ccgcagtatt
     6421 catcaccgcc atttgttcga agcttgaaaa gttcatcaga aattttatca aggtccccgg
     6481 tgaatggtac tatgtttctc aaatatccct catctgcagg aatgctttgc ttgccatatt
     6541 catataaagc tacattcaga ttaacaggcc gcccgttttt cttagaagtt gcaagctcgt
     6601 tcactatttt ccataattgg ctcttcgctt gatcaataag tccatccatg ctgccgcttg
     6661 tatctaatag taatgcgagt tgaatcgaac tatttttagc tggtttttta tcaaccattt
     6721 gattggagta agaaaaaatt gaatagataa aaacaaatgt caggataaat aaagatttca
     6781 ttacagcctc cgtaaaagga taattgattt ttgaaactga ggcttaaact gttgtgagaa
     6841 gaattttgtt ccagaataaa aagtattatt ctacaataaa attaaaataa gtttttgggt
     6901 agggttcatt tctcagtgta aaatgccacc actcttcaga aaggtttttg aaaccatatt
     6961 tatccattaa tgattttagc aataatctat tagctctttg ctgtggggtt agtttggggt
     7021 tttctgtatg cgaaagttta tgaaagcaat caaatccggt tcccatatca atactgttgt
     7081 ctttaaatct tttatcaaaa gattcaaaac attcacactg attatcaata tcaaattccg
     7141 gctgttgagg aagtggaatg ggaacaacgg ttaaatcaac tgtgctgccg cgactatgtc
     7201 ctgatttttc cgctatgtag ccatcgataa ataatctgct tttatctact gtaggataaa
     7261 attctttttt agtaagcgta tcagaaagat ctttagccca tctcacaaaa tgatctactg
     7321 cttgttgcgg gcgataagca tcataaattt ttagtgataa attaaaatgg cgtagttctg
     7381 cttgcacttt tgataaggaa tctgcagcgg ctttagtaat atagcacttt tgtgaaatgt
     7441 atccctgaac tggaacacca aggaaattat gatttgttgt atatcttaaa tctaaaataa
     7501 tatcaggtat tacttcacgg atttctacaa atccatcagg aatttcttga ttgaaatttg
     7561 gatgagttag agcaagagta atagaaataa ttaaaaataa ttttttcatt actcaaagat
     7621 aaaaaattat tcttgcttcc aatggcaatc agtacaaaga tgattttcta ttgattcgcc
     7681 taaattaaca ggatgctgga attttaatcc attaagatca acccgtgtat tgccggtatt
     7741 gtctgtttgc gagattattt tatgacagct gttgcaatcg tttgaaatta ctttgccatc
     7801 atcactaaca tgatttccat cgtggcatct aaaacaacca ggcgtatagg tgtgtgctat
     7861 attatccgga aaatgtttcc agttcgcatt catgtatgta aagtagtttc ttttataaat
     7921 cttttttact acatcaatac ttgttttaat tgcctctctt ttggaattat aaatttcagg
     7981 atagttggag ttgtaaaaat ctgtaattcg ctgttcaatg ctttcaagag cttgttgcgt
     8041 agttgaatat ttatcttcca gaacttctac agaaatactt tttatgtaag gtatggtctc
     8101 atcaactctg ttttttgaaa gcatttcatt catcatttta tcaggttgat gataaatatg
     8161 tgaagggcga ttgtggcaat cgatgcaatc cattgttctg aaattatctt tgttaaattt
     8221 cttttcatca aaatttgaat ttttatctct gaatatagtt tctttaccat tttttgattt
     8281 tactttaacc cagggaatta caagtcttct ctcgtcagta taaatatatt tgattacatt
     8341 atctttattt gcgtgccagt gaattccttc aatagaaccc aattcacttt taccaccacc
     8401 aattttaatc agcatactga tatttgattt tgtattctgt tcatccgaga gataataact
     8461 ataatcaact ttcttttcat tataaaaaag tgcgggagtg tggcactgtt cgcaagttcc
     8521 gattgccggt cgcagtgctt ctactggcgt tggaatcgga cgtgaatatt tattaaataa
     8581 tacagagtaa acctggtaag caccggatat ttttgattta acgtaccagt ttgttcccga
     8641 tccaatatgg caaccgacac aacccactct ggaatgtggt gaatctaaat atgcagtata
     8701 ctcaggctcc attaccttat gacatatagt tccgcaaaat tcatcggtct ctgtatattc
     8761 ataggcttta aaacttccga acgcggaaaa taacatcaat acgattgtac cgatagaaaa
     8821 ggttaaaaac atagctcgtt ttttcggatc attaagatca ataacaggaa gatatttccc
     8881 tcttattaat ccttttcgtt ctcttcttct ttcacggaag attccataag caattaataa
     8941 taagcctata atcaaaaatg ttgggattac aataaaagta agtattccca tataagtatt
     9001 ttcttctgtg ctgaagaaat caagaataaa caaaaatatt attaacccag aactaagagc
     9061 agcaattgca gccccaatta aggtaatagg attataaaca tatggaggaa aaatcttctt
     9121 catttaactt tttcatttat taaagttaag gagttaacta tctatacgca aagatcatag
     9181 catagattat ccatataatc atactgaatc caattattaa gactgtccat ccaaataatt
     9241 ttattgcttt cagaacaatt ggaggataag cttcaataag atatttattg aggacgccgt
     9301 ttcttacgat ctcttcatat tctttcggcc tgtctaactt aaattcctca agaggaactc
     9361 ttccggtaaa aatacaaaca tccataggaa atttttcggg tctaagatgt gtattaaaaa
     9421 agtgtacagt aaaaataaaa ccggtggcta ataaagcttc atcactatgg ataatagttg
     9481 caacatttat catgaatcct ggaatgaata gtgtgaaaaa ctctggaaac caaagaataa
     9541 aaccagtcga accaattact atcatacccc agaatacagc aaaatagtca aatttttccc
     9601 agtatgtcca tctaccatat tcaggacgtt caccttttcc tagaaaccat ttaatggacc
     9661 cgataaaatc gtttagatct tttttattga acatcattga gcggggtcct ataatcaaag
     9721 ctttaaaact tcctgcttct tttcttttat ttttaataag atcaacaata tgaataatga
     9781 aaacagttac catcactaaa gcagaaaatc tgtgaagata gcccgcagat tcaaaccctc
     9841 catataaact tgaaagaaag cgtgcccaac cggtatatga aaattttaaa gtcattccgc
     9901 tcaacgcaag atttaaaaaa cttaatatca taaaaaagtg gaggccgcga ttcaatcttg
     9961 taaatcttag tacttgtttt tcctgagtta ctgtttcatt atcaatttct aagttatttg
    10021 ctgtcgttat gttctcagtc ttcattttta ttttcactgt gtttcttttt taattctctt
    10081 cgccattgca atgatttagg taaccataaa agtgtatgta gtccggccaa aataaaagta
    10141 ccaagcaata atcctgtcat tccccaaaat gtccagaaaa gaacaggata tttttccggg
    10201 tcatggtggg tagcatgggt aaagtatcct gcaaactgtc tattagctga tggatgacag
    10261 ctcttacagg tttcaacaat attttttcta cttaaacgtg actttggatc gttaacacca
    10321 agaatatcat gggcaccatg acagtcataa cattttgctg tctttgtata acctaattga
    10381 gaaacttttc catgataagt atcaaaatat gtttctgaaa ttttttcgtg acattgtcca
    10441 cattgactca ttatggttaa tttaaatccc tcagcatctg ctcttgtaat actatgcgca
    10501 gagtggcaag tggagcatac tggtaatttt ttatctgttt tatttataag aggggaatga
    10561 atgctttttt caaacttttc ctctattcca tgatgacaat tcccgcaagt ctttgctata
    10621 tttgcctgat taacgctgct cataggatca tcaggtggaa gttctctgtg ggctgtatgg
    10681 caattagtgc aagtagctgt aact
//
LOCUS       DASWBY010000124         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_456500, whole genome shotgun sequence.
ACCESSION   DASWBY010000124 DASWBY010000000
VERSION     DASWBY010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456500"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VF870_02360"
     CDS             complement(<1..335)
                     /locus_tag="VF870_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9251051.1"
                     /translation="MQEKYIVSVDMGGTKILASVLNSKKGIIARQKKPTNISSGTKVY
                     VKDIAEIIFKVVSSSKLKKANIAAVCLGVPGSVNPKTGIIGFAPNLGIMNYKMKSELE
                     KLIPYPVLL"
     gene            464..1243
                     /gene="rsmA"
                     /locus_tag="VF870_02365"
     CDS             464..1243
                     /gene="rsmA"
                     /locus_tag="VF870_02365"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX9251052.1"
                     /translation="MNKVLPLKRFGQNYLQDQNIIKKIISEIDPSENDLIIEIGPGQG
                     AITQRLLESKANLTAIEIDKRVIDDLQNRFKDLHLLQSDFLKLDLNQFVNSSGQKLRV
                     AGNIPYNITSPILFKLFENNTIVKDAVFMVQYEVAKRMTAKMGSKDYGILSVLLEYFG
                     DTKLAFKVSPNVFYPKPNVHSAVVHINFNDKRNDSEFNLMFKSIVKSSFGNRRKTLKN
                     SLSNGIFTDVNFSDCGIDLSLRAEQLNVVDFIKLAEFASNK"
     gene            1272..2222
                     /locus_tag="VF870_02370"
     CDS             1272..2222
                     /locus_tag="VF870_02370"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9251053.1"
                     /translation="MNTIKSFIKKLKAFDVVVIGFSLFLVFLHIVFHSKIENSLTWIF
                     VNLVVICIAFGISYLEALNDQKVWRIIHYWYIAPIILLTFRQLFFMVKPIRVYDYDDW
                     FIAIDRWMFGTDPTHFLYQFSNPILTEILQIVYGMFYLLPIILCLSLLKKDRFIALDF
                     ALFSIIYGFFLSYLGYFSLPGIGPRFTLHDFNTINEMLPGLSLTNFLREMTNAGEGIP
                     AGTLNPAEVVQRDVFPSGHTMITLIVMYLSVRLRSRSRYFFIPIGTLLIFSTVYLWYH
                     YVIDLIGGLIFMIFSMWSCKLFFNWWRKKIGRELFEYEKH"
     gene            2228..>2855
                     /gene="ubiE"
                     /locus_tag="VF870_02375"
     CDS             2228..>2855
                     /gene="ubiE"
                     /locus_tag="VF870_02375"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX9251054.1"
                     /translation="MSEKKKQVKLMFDNIAGRYDFLNHFLSFGLDFYWRKKALKLTGL
                     NSQSILLDVACGTGDVAIQAKKMGVQKIYGADFSYNMLSLFDKKSEWVDGKLVQMVAE
                     KIPFKDNSVTNITVAFGVRNFYDIQEGFNSFYGILKPKGKATVIEFRMPSNKIIRTFY
                     KFYFKNILPVLGGIISGDKAAYTYLPDSVEEFDEKVNLKKLLTNSGFKE"
BASE COUNT          934 a          429 c          480 g         1012 t
ORIGIN      
        1 tcaagcagta ccggataagg aattaatttt tcaagttcag atttcatttt gtaattcatt
       61 attccaagat tcggtgcaaa gccaattatt ccagttttag gatttacaga tccgggtaca
      121 cctaaacaaa cggctgcaat atttgctttt ttaagttttg agctggaaac aactttaaaa
      181 attatttctg caatatcttt cacataaact ttggttccgc tgctaatgtt agttggtttt
      241 ttctgacgtg caataatccc tttttttgag ttaagtacag aggcaagtat tttggttcca
      301 cccatatcca cgcttacaat atatttttct tgcactgttt tctccttgaa tattggaata
      361 gaagtttaca ttggaaaact aaaagttatt tttttcttaa acaaacagat ttaaaatttt
      421 acttaagaaa attactatct tgttacagtt tttaaaaatg attatgaata aagttttacc
      481 actaaaaaga tttggtcaga attatctgca ggatcagaat attattaaaa agataatttc
      541 tgaaattgat cctagcgaaa atgatttaat aattgaaatt ggaccgggac aaggtgcaat
      601 tactcaaagg cttctagaaa gtaaagcaaa tttgactgca attgaaattg ataaacgagt
      661 tattgatgat ctgcaaaatc gttttaagga tttacattta cttcaatccg attttcttaa
      721 acttgatcta aatcaatttg taaattcatc cgggcaaaag ttaagggttg caggaaatat
      781 accctataac attacctccc caatattgtt taaactcttt gaaaataaca caattgttaa
      841 agatgccgtt tttatggttc agtatgaggt tgcaaaaaga atgactgcaa aaatgggatc
      901 aaaagattac ggaattcttt cagtacttct ggagtatttt ggagatacta aattagcatt
      961 taaagtttca ccgaatgttt tctatccgaa gccaaatgtc cattctgcag ttgtgcatat
     1021 caactttaat gataaaagaa atgattcgga atttaatttg atgttcaaat caatcgtaaa
     1081 atcttctttc ggaaatcgcc gaaagacact aaaaaattcg ttgagcaatg gtatatttac
     1141 cgatgtaaat ttcagcgatt gcggtatcga tctatcttta cgcgctgaac agcttaatgt
     1201 agttgacttc attaaactag ctgagtttgc aagcaataaa taaatccgtt tgtttcactt
     1261 aattctaatt aatgaataca ataaaatcct ttataaaaaa actaaaagct tttgatgttg
     1321 tagttatcgg cttttcactg tttttagttt tcctccacat agtctttcac agtaagatag
     1381 aaaattcatt aacatggata tttgtaaatc ttgttgtgat atgtattgct ttcgggattt
     1441 catatttaga agcattgaac gatcaaaaag tctggcgcat aatccattat tggtatatcg
     1501 caccaataat tttacttacg ttcagacaat tgttttttat ggtaaagcca atccgtgttt
     1561 atgattatga tgattggttt atcgcgattg accgctggat gtttggaact gatcccacac
     1621 attttcttta tcagttttca aatccgattc ttacagaaat actgcaaatc gtgtatggaa
     1681 tgttttatct tcttccaatt attctatgtt tatccttatt aaagaaggat agatttatcg
     1741 cactggattt tgctttgttc tcaattatct acggattttt cctttcttac ttaggatatt
     1801 tttctctgcc gggaattggt ccaagattta cacttcatga ttttaataca ataaatgaaa
     1861 tgcttccagg cttatcactt actaatttct taagggaaat gacaaacgcc ggagaaggta
     1921 tcccagctgg aacattaaat ccagcagaag ttgttcagcg agatgttttt ccaagcggcc
     1981 atacgatgat aacattaatt gtaatgtacc tttctgtcag attaagaagc cgcagcagat
     2041 attttttcat tcccatcgga accctattga ttttctcaac cgtgtacctt tggtatcatt
     2101 atgttattga tttaatagga ggattaatct ttatgatttt ttcaatgtgg agttgtaaat
     2161 tatttttcaa ctggtggaga aaaaaaatcg gcagagaatt atttgaatat gaaaagcatt
     2221 gatttatatg agtgagaaaa agaaacaagt aaaattaatg tttgataaca ttgccggcag
     2281 atatgatttc ttaaatcact tcttaagctt tggtttggat ttttattgga gaaaaaaagc
     2341 tttaaaactt accgggctga attctcaatc tattttgctt gatgttgcct gcggaacggg
     2401 cgatgtagca attcaggcaa agaagatggg agtccaaaaa atttatggtg cagatttttc
     2461 ttataatatg ttaagcttgt ttgataaaaa atctgaatgg gttgatggaa aacttgtaca
     2521 aatggttgct gaaaaaattc catttaaaga caattctgtt acaaacataa ctgtagcttt
     2581 tggtgttaga aacttttacg atattcagga aggttttaac tcattctatg gcatcttaaa
     2641 accaaaaggt aaagcaactg ttattgaatt cagaatgccc tcaaataaaa ttataagaac
     2701 gttttacaag ttttatttca aaaatattct gcctgttctt ggtggaataa tttccggaga
     2761 caaggctgct tatacttact tgcccgattc tgtagaagag tttgatgaaa aagtaaatct
     2821 taaaaaactt ttaacaaatt cgggatttaa agaaa
//
LOCUS       DASWBY010000125         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_681536, whole genome shotgun sequence.
ACCESSION   DASWBY010000125 DASWBY010000000
VERSION     DASWBY010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681536"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VF870_02380"
     CDS             complement(<1..929)
                     /locus_tag="VF870_02380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9251055.1"
                     /translation="MSDKIKLTVIGSGPGGYAAAFLAADLGMDVTLVDLDKNPGGVCL
                     YRGCIPSKALLHVAKLIHEAEEAKNWGIEFGEPKIDINKLRDFKNKVVDKLTSGLGQI
                     SKQRKINFIQGRASFINSSALKIEKVDGQTIEHTFEKAIIATGSVIATIPSLDIKSKR
                     LLNSTSALDLPEIPKSLLVVGGGYIGLELGSVYQALGTKVSVVEMMPGLLPGADRDLV
                     NYLSMRIKKSFAEIMTNSKVLEMKEVKDGISVKILDDKGNVADKVYDYVLMSIGRKPE
                     TRGLGLENTKVAVTDRGWIKVDKQLRTIDPNIFA"
     gene            complement(1033..>2356)
                     /locus_tag="VF870_02385"
     CDS             complement(1033..>2356)
                     /locus_tag="VF870_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX9251056.1"
                     /translation="AAPPAKSSGKKEILSFLLPDLGENIASADIVNVLVKAGDVIEID
                     QAILEIETDKATIEVPTNVAGKVVDVLVKAGSKVKVGDVVIKVETGAAAAEESKPEEV
                     KKEEIAESSIVEVKKETTPNLTGERASMQAAELDNQPPILKGAAPAAPSVRRIAREIG
                     VDINKVPGTGPNGRISMDDVKAFSKKLHESYSQGGGTGLNIKIESLPDFTKFGSINKV
                     EMTNIRKKTADHLGYAWATIPHVTQFDKADITLLEKTRKELNKNSEVKLTVTAILVRI
                     IVEALKKFPQFNSSIDMEKKEIIYKNYFNIGVAVDTEFGLIVPVIKNSDKKSLTEISV
                     DMNALAEKARNKKIGLDDLQGGSFTISNLGGIGGTYFTPIVNSPEVAILGVSRGNLEP
                     VWNGYGVFEPRLMLPLSLSYDHRIIDGADAIRFLRFVVEALEQPLRLL"
BASE COUNT          663 a          498 c          383 g          812 t
ORIGIN      
        1 gcaaaaatat ttggatcaat tgttctgagc tgtttgtcaa cttttatcca gccgcgatca
       61 gtaacagcaa cttttgtatt ctctaatcct agtcctcgcg tttcaggttt tcttccaatt
      121 gacatcaaca cataatcata aactttatct gcaacattac ctttatcatc cagtatctta
      181 acactaatac catcttttac ttctttcatt tcaagcactt tgctgtttgt cataatttca
      241 gcgaaagatt tcttaatcct cattgaaaga taatttacta gatcacgatc ggctccgggt
      301 aaaagtccgg gcatcatttc aacaacagaa actttagtcc caagtgcttg ataaaccgaa
      361 ccaagttcaa gtcctatata tccgccgcca acaaccaaaa gtgattttgg aatttcagga
      421 agatcaagtg ctgatgttga gttcaataat ctttttgatt ttatgtccag agacggaata
      481 gttgcgatta cagaaccagt cgcaataatc gctttttcaa aagtatgttc gattgtttgt
      541 ccatcaactt tttcaatctt taaagctgat gaatttataa agcttgctct gccttgaata
      601 aagtttattt ttctctgttt tgatatttgt cctagtccgc ttgtgagttt atcgacaact
      661 ttatttttaa aatcacgaag tttgtttata tcaatctttg gttcaccgaa ttcaattccc
      721 caattttttg cttcttcagc ttcgtgaata agctttgcaa catggagcaa agcttttgat
      781 ggaatgcatc cacggtaaag acaaacacca ccgggatttt tatccagatc aacgagagta
      841 acatccattc caagatcagc agcaaggaac gctgcagcgt aacctccggg cccgctgccg
      901 ataactgtta attttatttt atcactcatt ttaaattcct ttagaagaat agaacgcgga
      961 taacacggat ttaacggata aagaccgatc agtgaaaacc cgcgtgatct gcgttatccg
     1021 tgttctattg gtttataata atctaagagg ctgctcaagt gcttcaacaa caaatcttaa
     1081 aaatctaatc gcatctgccc catcaataat tctgtgatca taagacaatg ataagggaag
     1141 cattagtctt ggttcaaaaa ctccgtagcc attccaaaca ggttctaagt ttccacggga
     1201 tactccaaga attgcaactt ctggtgagtt aacaatcgga gtaaaatatg ttccaccaat
     1261 tccaccaaga ttggaaattg taaaacttcc tccctgcaaa tcatctaatc ctattttttt
     1321 attacgagct ttctccgcaa gcgcattcat atcgacagaa atttccgtta aagatttttt
     1381 atccgagttt ttaatgactg gaacaatcaa tccaaactca gtatcaacag caacaccaat
     1441 gttaaaataa tttttgtata tgatttcctt tttctccata tcaatactgg aattgaattg
     1501 aggaaacttc tttaacgctt caacaattat tctaacaaga attgccgtta cagttaactt
     1561 cacttcagaa tttttattta actcttttct tgttttctca agcaaagtaa tatcagcttt
     1621 atcaaattgt gtaacgtgcg ggattgtagc ccaggcataa ccaagatgat ccgcggtttt
     1681 tttgcggatg tttgtcatct caactttgtt gattgaacca aactttgtga aatcaggaag
     1741 agattcgatt ttaatattta atcctgttcc gccgccctgt gaatatgatt cgtgtaattt
     1801 cttggaaaat gccttaacat catccattga aattctgccg tttggtcccg tgcccggcac
     1861 tttattaata tcaacaccaa tttctcttgc aattcttctt acggatggag ctgccggagc
     1921 ggcgcctttt aatattggcg gttgattatc caattctgct gcctgcattg aagcacgctc
     1981 acctgttagg tttggtgtag tttctttttt tacttcaact attgacgatt ctgcaatttc
     2041 ttctttttta acttcttccg gctttgattc ttcagctgct gctgcacctg tctcaacttt
     2101 aataacaaca tcgccgacct ttactttact tccggctttt accaaaacgt caacaacttt
     2161 tcctgcaaca ttagttggaa cttcaatagt tgctttgtct gtttcaattt caagtattgc
     2221 ctgatcgatt tcaatgacgt cgcctgcttt tacaagtaca tttacaatgt ctgcagatgc
     2281 aatattttcg cctaagtcgg gtaatagaaa acttaaaatt tcttttttgc cggatgattt
     2341 ggctggtggt gcagct
//
LOCUS       DASWBY010000126         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_118490, whole genome shotgun sequence.
ACCESSION   DASWBY010000126 DASWBY010000000
VERSION     DASWBY010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118490"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VF870_02390"
     CDS             <1..1300
                     /locus_tag="VF870_02390"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX9251057.1"
                     /translation="FQISGVTTKDYPELVNAFVLVKLAAARANYDSGALPKYVLDAVE
                     KACNEVLAGKYHDQFLVDLYQGGAGTSANMNTNEVLANIALELSGKQKGDYAYIEPHD
                     HLNMSQSTNDSYPTSLHVAIILMNQKLLPELEALIKSFEAKGKEFENILKMGRTELQD
                     AVPMTLGQEFIAFASQLKASIDELKQAESYLYVINMGGTAIGTKLTAQKGFVENCAKH
                     LGELLKKKIVPAKDLIAATSDLSGFVNYSSALNTLSVRLSKIASDLILLTSGPRTGLF
                     EINLPALQPGSSIMPGKVNPVIPELVNLVAFRVMGNDVAVTIASKTGQLQLNAYEPLA
                     ISCILESQKLLTNTMKTFRTSCVDGITANEDVMTKYINQSVGIVTALNPVLGYEKTTE
                     LAKEALETGKGILELIREKKLLTEDQIKELLDPKKMTGQE"
     gene            1397..>1943
                     /locus_tag="VF870_02395"
     CDS             1397..>1943
                     /locus_tag="VF870_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251058.1"
                     /translation="MKDDLSQKDLTQITISVITILLLIVGSVWVFMPFVAALLWASII
                     VISTYNFMLRLQKLMWNKKGLAVTVMVLIILMIILVPIAVVFGAIAMNMSEISLWFGS
                     LSEIKLQSAPEWMAGIPIVGSQLANGWNQLASLNNDEIIKRIIPFLDDGLKWFVAKAG
                     GFGLFMIQFLITVIICGILYSK"
BASE COUNT          662 a          312 c          370 g          599 t
ORIGIN      
        1 ttttcaaatc agtggtgtaa ctactaaaga ttatcctgaa ttagtaaatg catttgttct
       61 tgtaaagctt gctgctgcta gagcaaatta tgactcaggt gcattaccaa aatacgtcct
      121 tgatgcggtc gaaaaagcct gtaatgaagt tcttgcagga aaatatcatg atcaatttct
      181 agttgatttg taccagggtg gtgcaggaac ttcagcaaat atgaacacaa acgaagtgct
      241 ggctaatatt gctcttgaac taagcggaaa acaaaaagga gactatgctt acattgaacc
      301 gcacgatcat ttaaatatgt ctcagtcaac caatgattcc tatccgactt cgctccatgt
      361 tgcgattatt cttatgaatc aaaaactact tcctgaactt gaagcattaa taaaatcttt
      421 tgaagcaaaa ggaaaagaat ttgaaaatat tttgaagatg ggaagaactg aattacagga
      481 tgctgttcca atgacactcg gacaagaatt cattgctttt gcttcacagc ttaaagcctc
      541 tattgatgaa ctcaaacaag cagaatcata tctttatgtc attaatatgg gtggaacggc
      601 aattggaacc aaactcactg cacaaaaggg atttgtcgaa aattgtgcaa aacatcttgg
      661 agaactcctg aaaaagaaaa tcgttccagc aaaggatttg attgcagcaa catctgatct
      721 ttcaggcttt gttaattact caagcgcttt aaatacactt tcagtaagac tttcaaaaat
      781 tgccagcgat ttaattttat taacttcagg tccaagaacc ggtttgtttg aaataaatct
      841 gccggcacta caaccaggtt cttcaattat gcctggaaaa gttaatccgg taattccgga
      901 attagtaaat cttgtggcat tcagagttat gggtaatgat gttgcggtaa ctattgcttc
      961 gaaaacaggt caacttcaat tgaatgctta tgaaccactc gcaatatcct gtatacttga
     1021 atcacaaaaa cttctaacaa atacaatgaa aactttcaga acaagttgtg ttgatggaat
     1081 tacggcaaac gaagatgtaa tgacaaaata tattaatcaa agtgttggta ttgtaacagc
     1141 actaaatccg gtgcttggtt atgaaaaaac aactgagctt gcaaaagaag ctcttgaaac
     1201 agggaaaggg attcttgaac tcatcagaga aaaaaaatta ttaacagaag atcagataaa
     1261 agaattgctc gatcctaaaa aaatgacagg gcaagaataa tatttaaatt gaagtattat
     1321 atggcaccgg agtgaaatca ggttgaatta acagtcaatt agaaaattat ttgtacattt
     1381 ttttgagagt aaacttatga aggatgatct ttcgcaaaaa gacctaaccc agattacaat
     1441 cagtgttata actattcttc ttcttattgt aggaagtgtt tgggtattta tgccatttgt
     1501 tgctgctttg ctatgggcat caataatagt tatttcaaca tataatttta tgttgagatt
     1561 gcagaagctg atgtggaata agaaaggact tgcagtaact gtaatggttt taataatcct
     1621 gatgattatt ctggttccga ttgctgtagt gtttggagca atagctatga atatgtctga
     1681 aatttcgctt tggttcggtt cattgtcaga aattaaactt caatcagcac ctgaatggat
     1741 ggcaggaatt cccatagttg gatcacaatt agcaaacgga tggaatcaac ttgcttccct
     1801 aaacaatgat gaaattataa aacgtattat tccgtttctt gatgatggat taaaatggtt
     1861 tgtggctaaa gcaggaggat ttggactatt tatgatccaa ttcctgatta ccgttataat
     1921 ttgtggtata ctttactcca aag
//
LOCUS       DASWBY010000127         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_42230, whole genome shotgun sequence.
ACCESSION   DASWBY010000127 DASWBY010000000
VERSION     DASWBY010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42230"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1456
                     /gene="fumC"
                     /locus_tag="VF870_02400"
     CDS             62..1456
                     /gene="fumC"
                     /locus_tag="VF870_02400"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX9251059.1"
                     /translation="MDFRIEKDTMGEIQVPADRYWGAQTQRSKENFKIGGDRMPVEII
                     RAFGILKKAAAQANAELGVLSEEKAKIISQVCDEIIEGKLEDHFPLVVWQTGSGTQSN
                     MNVNEVIANRAHVLLGGKLGEGKHFIHPNDDVNKSQSSNDTFPTAMHVAACDVITHVT
                     IPGVTKLLNTLKKKAVEFEGIIKIGRTHLMDATPLTLGQEFSGFAAIVEHGLRAIRYS
                     MTHLHQIALGGTAVGTGLNTPKGFDVLVAKKIADLSPCNFVTAENKFEALSAHSSIIE
                     ISGALKLLATGLTKIANDIRFYASGPRSGIGELLLPENEPGSSIMPGKVNPTQCEALT
                     QVCTQVVGNDITITMAGMQGHFQLNVFKPVMILNLLNSARLLGDACVSFNDNCAIGIK
                     PNLERIKFNLDNSLMLVTALNPVIGYDNAAKVAKKAHKENKTLREAALELGLLTAEKF
                     DEVVRPEKMIGPNK"
     gene            complement(1523..1948)
                     /locus_tag="VF870_02405"
     CDS             complement(1523..1948)
                     /locus_tag="VF870_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251060.1"
                     /translation="MKKRVLLLFVFAVSFFPGIINTVFAQNEPVLYFCEKYDSKKGEI
                     GVSDRFTTGYLTVMVKSDNELGLEDVHIQFDKLNERSKNFEFYKKFDYVIDPEMSYVF
                     FAKNSDSDLKFEEPGFYRVFLLDDNDKTVASALIEIVSK"
BASE COUNT          777 a          369 c          407 g          714 t
ORIGIN      
        1 gtagtatttt aaaatcatta gatcgtaatt aatttttatc ataaatattc aggagaaaaa
       61 aatggacttc agaattgaaa aagatacaat gggtgaaatt caagttcctg ctgataggta
      121 ttggggggca cagactcaaa ggtctaaaga aaattttaaa attggtggag atagaatgcc
      181 ggtggaaatt atccgtgcgt ttggaatatt aaaaaaagca gctgctcaag caaatgctga
      241 attaggtgta ctgtcagaag aaaaggctaa gataatcagt caggtttgtg atgaaataat
      301 agaaggaaag ttagaagatc attttccgct ggtagtatgg caaaccggtt caggtacgca
      361 atccaatatg aacgttaatg aagtaattgc taaccgtgct cacgtattac taggtggaaa
      421 acttggggaa ggaaaacatt ttatccatcc aaatgatgat gttaataaat ctcaatcttc
      481 aaatgacacc ttcccgacag caatgcatgt tgcagcctgt gatgttatta cgcatgtaac
      541 aattcccggt gttaccaaac ttttaaatac acttaaaaag aaagctgtgg aatttgaagg
      601 tataattaaa attggcagaa cacatttaat ggacgcaact cctcttactt tgggtcagga
      661 attttccggt tttgcagcta ttgttgaaca cggattgaga gcaatcaggt attccatgac
      721 acatttacat caaatagcac ttggcggaac agcagttggt actggtttaa atacaccaaa
      781 ggggtttgat gttttagttg caaaaaaaat agctgatctc tcaccatgta attttgttac
      841 tgctgagaac aaatttgaag cactttctgc ccatagttca attatagaaa tttcaggggc
      901 acttaaactt ttagcaacag gtttgaccaa aattgccaat gatatccgtt tctatgcatc
      961 tggtccaaga tcaggtatcg gagaactgct acttcctgaa aatgaaccag ggtcttcaat
     1021 tatgcctgga aaagttaatc caactcaatg tgaagcctta acacaggttt gcacacaggt
     1081 tgtaggcaat gatattacaa tcacaatggc tggaatgcaa gggcatttcc aactaaatgt
     1141 ttttaaacct gttatgattc ttaatctgct taactcagcc agattattag gagatgcctg
     1201 tgtttcattt aatgataatt gcgccatagg cattaagcca aacctcgaaa gaattaagtt
     1261 taatcttgat aattctttaa tgctggtaac tgctttaaac ccggtaattg gttatgataa
     1321 cgcagcaaag gttgccaaaa aagcacacaa agaaaataaa actcttagag aagcagcttt
     1381 agaacttgga ttactgaccg cagaaaaatt tgatgaagta gtaagacctg aaaaaatgat
     1441 tggaccgaat aaataagtta aatgtttctt taaaattaaa aacggcagcc ttttgactgc
     1501 cgtttttgtt taatgcttaa agttatttag aaacaatttc aattaatgca cttgctactg
     1561 ttttatcgtt gtcatccagt aggaacactc tgtaaaatcc cggctcttca aattttaaat
     1621 cactatcaga atttttagcg aagaaaacgt aactcatttc cggatcaatt acataatcaa
     1681 attttttata gaattcaaaa ttcttgctgc gttcatttaa tttatcaaac tgaatgtgaa
     1741 catcttcaag gcctagttca ttatctgatt ttaccattac ggtcaaataa ccggttgtaa
     1801 atctatcact tacgccaatc tcaccttttt tagaatcata tttttcacag aaatagagaa
     1861 ctggttcatt ctgagcaaat actgtattta ttattccggg aaagaaagaa actgcaaaaa
     1921 caaaaagtaa caaaaccctt tttttcatct agatatcctt tataattagt gtggagtaac
     1981 ttaccgtaac ccgattccat taaaaaataa ttttacttca attaaagggt ggaaattata
     2041 gcattattgc tataggaaat cattattttt ggcttcaaaa atttggaatg cttttggaaa
     2101 cctggaatgg aataatatca ctacttatag cttgcattga atttctttta cttttcaatc
     2161 tattaatatt tattgagaag aacagaataa acatcattgc aatgattatg attacgttgc
     2221 ttgcatctta ccagacgatg gaatttttaa tgtgccaggt tgaattg
//
LOCUS       DASWBY010000128         4661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_605993, whole genome shotgun sequence.
ACCESSION   DASWBY010000128 DASWBY010000000
VERSION     DASWBY010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605993"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VF870_02410"
     CDS             complement(<1..243)
                     /locus_tag="VF870_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251061.1"
                     /translation="MNKKLHLIYVGSFFVVGVAVLVLLAINGYQYYSTPIEERFFNPS
                     HAALKPSGNWGHGFGIIGTLMMIFGVAIYMIRKRSRK"
     gene            complement(336..641)
                     /locus_tag="VF870_02415"
     CDS             complement(336..641)
                     /locus_tag="VF870_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251062.1"
                     /translation="MSKVLFTIQYEIVPEKREDYLKVVKELKNLLKAEGLESYDVYEV
                     KGKAVGFQELYTFTSMEAYEAFDDLNDERINILVDKISGMSVDKTTKYNTLVQLADE"
     gene            complement(656..1522)
                     /gene="dapF"
                     /locus_tag="VF870_02420"
     CDS             complement(656..1522)
                     /gene="dapF"
                     /locus_tag="VF870_02420"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX9251063.1"
                     /translation="MQKIFFKKMSGAGNDFIFIDKNQNPGLELTAEKIKDLCNRRNGI
                     GADGVITIEDKADYSYKMVYYNADGSTGSLCANGARCSIWFAEKTLRLKNGIAKFISN
                     DAEFSGEVLDDELIKFNLNSPKQIKYNFKIKAFGQMITSNFADTGSPHVVIKISDVLK
                     DAANPKSFFKNILDFPVFNLGKEIRYCSDFKPGGTNVNFVDVIDDVIHIRTYERGVED
                     ETLACGTGSVAAALICFVTDKLKPPIKLKTYGGDYLTVNFELENQKVKNLSLTGPAKI
                     IFEGSIDEKLFI"
     gene            complement(1669..2022)
                     /locus_tag="VF870_02425"
     CDS             complement(1669..2022)
                     /locus_tag="VF870_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEX9251064.1"
                     /translation="MPNIFQLLPSLEGEEMSYAQSLINDMTDAQAQQFAMAYSARRKD
                     PTTILILALVGFVGFAGLHRFMLNQIGMGILYFLTGGLCFIGTIIDLVNHRKLAFEYN
                     STVAQQVAMMVKQLK"
     gene            complement(2032..2388)
                     /locus_tag="VF870_02430"
     CDS             complement(2032..2388)
                     /locus_tag="VF870_02430"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX9251065.1"
                     /translation="MNNIDQIRLAEFNKIPLSIIGYIRLIGLEGIIWITALIYLAFFI
                     NPFETHFTICPLANAGFEHCPGCGLGNSISLFFHGYFKESINTHVLGIPALFIILHRI
                     YSIIRFNLKKVKTTIQ"
     gene            complement(2546..2905)
                     /gene="ssrA"
                     /locus_tag="VF870_02435"
     tmRNA           complement(2546..2905)
                     /gene="ssrA"
                     /locus_tag="VF870_02435"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2938..3486)
                     /locus_tag="VF870_02440"
     CDS             complement(2938..3486)
                     /locus_tag="VF870_02440"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX9251066.1"
                     /translation="MKIYTKTGDKGETGLFGGERVSKNNLRLNAYGTIDELNSFLGLA
                     IIEVTCGEIKDVLIDLQNKLFVLGADLATPETEKNSKLNITRLPELYITEAEQAIDKF
                     DAQLDELKNFILPGGSKGSALLHICRTISRRAEREVVALKNTEHIGENIVIFLNRLSD
                     LFFVLSRFENKYSNIPDTKWIP"
     gene            complement(3492..>4661)
                     /locus_tag="VF870_02445"
     CDS             complement(3492..>4661)
                     /locus_tag="VF870_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX9251067.1"
                     /translation="LIFAHGIFPLSYPKKQKIYNGVIMFELTNTNSDMLSFFVSLGAK
                     NELINGVTHIQSYTSLEDEVYSLNNGVALKFMNTSSIIELKGTDSLDFLHRISTNSMK
                     NLNKEEVKKTIFTSEKGRIIGVSTVMNFESYILLITGIYSKQKTMSWINKYIIGDDVK
                     LSDASHRFNLFEIVGPQSESFLSLFIGDALNAVPDNSFKVVSADGVLFFLARLIDENG
                     LQKFWILAEQENGKKLVGNMFENKGPYDFNLVGEDAYNVYKIENGIPSDPNELNDLYN
                     PHEAKIINLVDFKKGCYIGQEVIARLDTYDKVQKHLVELCFPEAIETNEKFTLLDDQM
                     NEVGVVTSISYSPKIKKNIALGYLKKSLAVHGTKILAKNETKSMEVMIHDLPFKK"
BASE COUNT         1561 a          836 c          681 g         1583 t
ORIGIN      
        1 ttttctggat cgcttacgaa tcatataaat tgccacccca aaaatcatca ttagtgtgcc
       61 gataattcca aaaccatggc cccagttgcc gcttggtttt aatgcagcgt gtgatggatt
      121 gaaaaatctc tcttcaattg gagttgagta gtactgatag ccattaatag ctaatagtac
      181 taaaacagct acgccaacaa caaaaaaact tccaacataa attagatgta gttttttatt
      241 cattcgagtt aaatggatat aattagtaag tgatttaaat taagaaaagt aacattgaaa
      301 aaaaatgaag gggtctggtt agagacccgc taaatttatt catctgcaag ttgaacaaga
      361 gtgttgtatt ttgtggtttt atcgactgac ataccactta ttttatctac aaggatattt
      421 atgcgttcat cattcaagtc atcaaaagct tcataagctt ccattgaagt gaaagtgtat
      481 agttcctgaa aaccaacggc ttttcctttt acctcgtaaa catcgtatga ttccaggcct
      541 tctgctttaa gaagattttt taattcctta acaactttta aataatcttc tcgtttctca
      601 ggaacaattt catactgaat tgtgaataga actttagaca tttttactcc aataattaaa
      661 taaataattt ctcatctatt gaaccttcaa aaataatttt tgcaggtccg gttaaagaca
      721 gatttttaac tttttgattt tcaagttcaa aattaactgt aagataatca ccaccataag
      781 tttttaattt aattggcggt ttaagcttat cagtaacaaa acaaattaaa gcagctgcaa
      841 cagaaccagt tccgcaagca agtgtttcat cttcaacacc gcgctcataa gttctgatat
      901 gaataacatc atcaataaca tctacaaaat ttacatttgt gccgccgggt ttaaaatcac
      961 tgcaatatct aatctctttg ccaagattaa aaacaggaaa atccaaaata tttttaaaaa
     1021 atgatttagg atttgcagca tctttaagaa catctgatat tttgataaca acatgcggtg
     1081 atcctgtatc tgcaaaattt gatgttatca tctgcccaaa agctttgatc ttaaaattgt
     1141 atttaatctg ctttggtgaa ttaagattaa acttaattaa ctcatcatcc aaaacttcac
     1201 cggaaaattc tgcatcattt gatataaatt ttgcaattcc attttttaac cgtaaagttt
     1261 tttctgcaaa ccaaattgaa catcttgccc cgttagcaca aaggctgcca gtcgaaccat
     1321 cggcattata gtaaaccatt ttgtaactat aatctgcttt gtcttcaatg gtaattacac
     1381 catccgcccc aattccgttt ctccggttgc aaagatcttt aatcttttct gctgttaatt
     1441 ccagtcccgg attctgattt ttatcaataa agataaaatc atttcctgca ccgctcatct
     1501 ttttaaaaaa aatcttttgc attcaactgc taatttagtt taatgcttta agcttttcaa
     1561 taaaaattat tgttaaatat aagttattta ttaagaattg ataagaaaat attagaggaa
     1621 tacctgcaga catttttcaa tctgcaggaa aaaaatgccg aatagaattt atttaagttg
     1681 cttaaccatc atggcaacct gctgtgcaac agttgaatta tattcgaatg caagttttct
     1741 gtggttaacc agatctatta ttgtacctat aaaacataaa ccaccggtta gaaaatatag
     1801 tatacccata ccgatttggt taagcataaa tctgtgcaga ccggcaaatc caacaaaccc
     1861 aaccaatgcc aaaattaaaa tagttgttgg atcctttctg cgtgcactgt aagccattgc
     1921 aaattgctgt gcctgtgcat cagtcatgtc attaataagg ctttgagcat aagacatttc
     1981 ttcgccttca agacttggta ataattgaaa aatatttggc ataattactc cttattgtat
     2041 tgtggttttt acttttttaa gattaaatct gatgattgaa taaattctat gaagaatgat
     2101 gaagagtgca ggtattccta aaacgtgagt gttaatagat tccttgaaat atccatgaaa
     2161 gaacaacgaa atagaattgc ctaatccgca gccaggacaa tgttcaaatc ccgcattggc
     2221 taaaggacaa attgtaaaat gtgtttcgaa cggattaatg aaaaaagcca gataaataag
     2281 agctgtaatc cagataattc cttctaaacc aattaagcga atgtaaccta taatgctcaa
     2341 aggaatttta ttgaattcgg ctaaccttat ttggtctata ttgttcatca tcaccatgaa
     2401 ttgaattact atttaaaact tacttaataa ttaaaaccaa atcaatttca ggatgatgaa
     2461 cggtatgtat ttatcggtga gtgaaactta aaaaaggatt tattttagat ataattttac
     2521 tgaatttgaa ataagtactc caaatttgtg gaggtggcgg gagtcgaacc cgcgtccgaa
     2581 attatgatcc aaagaacgtc tacatacata gtctgtttca tttttgactt taagctgcca
     2641 aacagacaaa gatcacttaa agcctacttg ctgttatttc gcctttcctg cgcaagagac
     2701 agaatcagct attgtatcta aacgtcgttc tatcctagcc cgatacaaga agataagcag
     2761 aacggctgtt aactattaat taagcagcta aggcgtaatt agattcgcca gttattgttt
     2821 tttcccaatt catttacgtg tattcgggga acacggtacg cagttctaag tctctttaat
     2881 cccgtcgaaa ccaaaattca cccccgtaat cagtctgcag tctgcaagat tataaaatta
     2941 cggaatccat tttgtgtctg gaatgtttga gtacttattt tcaaaacgtg aaagaacaaa
     3001 aaacagatca gataatctgt ttaaaaatat aacaatgttt tcaccgatat gttcggtatt
     3061 ttttaatgcc acaacttctc tttcagcacg acgtgaaatt gtgcggcaaa tatgcaaaag
     3121 agctgaacct ttacttccgc caggtagaat gaagttttta agttcatcta attgagcatc
     3181 aaacttatca atagcctgtt cagcttcagt aatatataat tcaggtaacc gggtaatatt
     3241 taattttgaa tttttttctg tttccggagt tgcaagatct gctcctaaaa caaataattt
     3301 attttggaga tcgataagta cgtcttttat ttcaccgcaa gtaacttcaa taatcgctaa
     3361 acccaaaaaa gaatttaatt catctatagt gccgtatgca tttaatctta aattattttt
     3421 tgaaactctc tcaccgccaa ataatcctgt ttcaccttta tcaccagtct ttgtatatat
     3481 tttcatcttt tttatttctt gaatggaaga tcatggatca taacttccat agatttagtt
     3541 tcatttttcg ctaaaatttt tgttccatga acagccagtg atttttttag ataacctaaa
     3601 gcaatattct ttttaatttt aggagaatat gatattgatg taacaacacc aacttcgttc
     3661 atctgatcat ctaacaaagt aaacttctca ttagtttcaa ttgcttcagg gaaacataat
     3721 tcaactaaat gtttttgaac tttatcataa gtatctaatc ttgctattac ttcctgccca
     3781 atataacaac cttttttaaa gtcaaccagg ttaatgattt ttgcttcgtg cggattatat
     3841 aaatcattta attcattggg atccgaaggg ataccattct caatcttata aacattgtaa
     3901 gcatcttcac caactaaatt aaaatcatag ggacctttat tttcaaacat attgcccact
     3961 aatttcttac cattttcctg ttcggcaaga atccagaatt tttgcaatcc gttttcatca
     4021 ataagtctgg caagaaaaaa taatactcca tctgcactta ctactttaaa tgagttatca
     4081 ggaactgcat tcaatgcatc gccaataaac aaagacaaaa atgattcaga ttgaggacca
     4141 acaatctcaa acaaattaaa tcgatgactt gcatcgctaa gtttcacatc atcgccaata
     4201 atgtatttgt taatccagct catagttttt tgtttgctat atatgcctgt tatcagaaga
     4261 atataactct caaaattcat tacggtagaa acgccaatta ttcttccttt ttctgatgta
     4321 aaaattgttt tctttacttc ttctttatta agatttttca ttgaattagt agaaatcctg
     4381 tgtagaaaat caagagaatc cgttcctttt aattcaataa tagaagaagt attcataaac
     4441 tttaacgcaa ccccattatt aaggctataa acttcatcct caagtgaagt ataactttgt
     4501 atatgagtta caccattaat caattcgttt ttagccccaa gggaaacgaa gaatgataac
     4561 atatctgaat ttgtattagt caactcgaac attattactc cattgtatat cttttgtttt
     4621 ttcggataag aaagtggaaa aatcccgtgt gcaaagataa g
//
LOCUS       DASWBY010000129         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_418588, whole genome shotgun sequence.
ACCESSION   DASWBY010000129 DASWBY010000000
VERSION     DASWBY010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418588"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VF870_02450"
     CDS             complement(<1..632)
                     /locus_tag="VF870_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9251068.1"
                     /translation="MDKITEKKANKTNGFIALFIVIALFALDIFLLVTGVRTNDDSIL
                     WFFIPLVIISFISFGGFTIVQPNESKVLILFGKYIGTVREDGFWWVNPFTLKKHVSLR
                     IRNFNSDKIKVNDLHGNPIEIAAVIVWRVVDSARAIFDVQNYEQFVAIQAETAIRTLA
                     SEYPYDTNEGEKESLRSSPTEVAENLQKELQKRLEVAGVTIVEARITHLA"
     gene            795..2252
                     /locus_tag="VF870_02455"
     CDS             795..2252
                     /locus_tag="VF870_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9251069.1"
                     /translation="MKILLTGATGYIGKRLLPVLLEKGHEVVCCVRDKNRFPTEGIYK
                     QQNVSVFEVDFLKDTSNSGSSLSIIKNIDAAYYLIHSMSSNVKDFGLLEETSANNFIK
                     LVKQTSVRQIIYLGGITNEEKLSKHLASRKKVEEILSKSGIPLTSIKAGIIVGSGSAS
                     FEIIRDLVEKLPAMITPKWLNTKHQPIAIRNILEYLTGVLLKPETFNRSFDVGGPDVL
                     SYKQMLLQLAEVRGLKRFIFTVPVMTPRLSSYWLYFVTSTSYMLAINLVNSMKIEVVA
                     KDNELEKMLGIKPISYKEAVQLAFQKIEQNNVVSSWKDSLVSSYVDNSLLEHINVPTN
                     GCFVDKREKEIATTVNQVLDNFWSIGGERGWYYADWLWHIRGFLDKLVGGVGLRRGRT
                     NKNEIHTGDTLDFWRVLAADKQNKRLLLYAEMKIPGEAWLEFKIVEKNGKIFLQQIAT
                     FRPKGLLGRMYWYLVLPFHYFVFDGMAENIVKHSN"
     gene            complement(2348..2479)
                     /locus_tag="VF870_02460"
     CDS             complement(2348..2479)
                     /locus_tag="VF870_02460"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF015904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX9251070.1"
                     /translation="MVNELISVQGKPVDIGGYFMPDDKKAFAVMRPSAKFNKIIDEM"
BASE COUNT          820 a          443 c          441 g          787 t
ORIGIN      
        1 tatgcaaggt gagtaattct tgcttcaaca attgtaactc ctgcaacttc caatcttttt
       61 tgaagttctt tctgaagatt ctcggcaact tctgttggac tgcttcttaa agattctttt
      121 tctccttcgt ttgtgtcgta aggatattct gaagcaagtg tacgtatagc tgtttcagcc
      181 tggatggcaa caaattgttc gtaattctga acatcaaaaa ttgcacgggc agaatctaca
      241 acccgccata caattacagc ggcaatctca atcgggtttc cgtgtaaatc atttacttta
      301 attttatcgc tgttaaagtt tctaattcgt aaagaaacat gtttctttaa tgtaaaagga
      361 ttaacccacc agaaaccatc ttcccgaacg gtaccaatat atttaccaaa caggatcaac
      421 acttttgatt cgtttggttg aacaattgta aatccgccaa agcttataaa tgatattata
      481 acgagtggta taaaaaacca tagaattgaa tcatcattag ttcggacacc tgtgaccaaa
      541 agaaaaatat ccaaagcgaa aagagcgatt acgataaata aagcaataaa accatttgtt
      601 ttgttagctt ttttttctgt tattttgtcc atcttgcatt cctttataaa attgctatcg
      661 taatgatatc atattgatag caaataatca agtcctaagt gaaaaatatt ataaacaact
      721 aatatttagt cttttaactg cttttgaatt aaatatccgt acattttgtt aggaaaagta
      781 attattaagg atttatgaaa atattattaa ccggggcaac cggatacatc ggcaagcggc
      841 tcctacctgt tttacttgaa aagggacacg aagttgtttg ctgtgtgcgc gataaaaacc
      901 gatttcccac agaaggaatt tataaacaac aaaatgtttc tgtatttgaa gttgattttc
      961 ttaaagacac ttcgaactca ggctcaagtt taagtataat taaaaatatc gatgcggcat
     1021 actacctcat tcactcgatg agttccaacg ttaaagattt tggtttactc gaagagactt
     1081 ccgcaaacaa ttttataaaa ctagttaaac aaacatcagt ccggcagatc atttatcttg
     1141 gtggaatcac aaacgaagaa aagctttcca aacatcttgc atccagaaaa aaggttgaag
     1201 agattttaag taaaagcggc attccgctta catcaatcaa agcaggaata attgttggat
     1261 ccggcagtgc atcatttgaa attattcgtg atcttgtcga aaaacttccc gcgatgatca
     1321 ctcccaaatg gcttaataca aaacatcaac ccattgcaat aagaaatatt cttgaatact
     1381 tgaccggagt tttacttaaa ccggaaacat ttaacagatc atttgatgtt ggcggaccgg
     1441 atgttttatc ttataaacaa atgctattgc agctagcaga agtacgcgga cttaaaagat
     1501 ttatttttac cgttccggtt atgactcccc gactttcatc ttactggctt tactttgtta
     1561 catcaacatc atatatgctg gctataaatc ttgttaacag tatgaaaatt gaagttgtag
     1621 caaaggataa tgaacttgaa aaaatgctcg gcataaaacc aatatcatac aaagaagctg
     1681 tgcagcttgc atttcaaaag attgaacaaa ataatgttgt ttcaagctgg aaggattcac
     1741 ttgtttcgag ttacgtggat aattctttgc ttgagcatat taacgtaccg acaaatggat
     1801 gctttgttga taaaagagaa aaagaaattg ccacaactgt aaatcaggtt ttggataact
     1861 tttggtcaat tggcggtgag agaggctggt attatgctga ttggctatgg cacatacgcg
     1921 gcttccttga taagcttgtc ggcggggttg gtttgagaag aggaagaaca aacaaaaacg
     1981 aaatacacac tggcgacaca cttgactttt ggcgtgtgct tgcagcagat aaacaaaaca
     2041 aacgattgct tctttatgcc gagatgaaaa taccgggtga agcctggctt gagtttaaaa
     2101 ttgttgagaa aaatggaaaa atttttttac aacaaatcgc gacattccgc cccaaaggtt
     2161 tacttggcag aatgtattgg tacttggtat tgccgtttca ctattttgtt tttgatggaa
     2221 tggcagagaa tattgtaaag cattcaaact aatttatatc tcctaaatca tttaaaaaca
     2281 aaaacggaca aagaaatttg tctttgtccg ttactgattt caaaccattc ggttcagagt
     2341 cttttaatta catctcatca attattttgt taaactttgc acttggacgc ataactgcaa
     2401 aggctttttt atcatcaggc ataaagtaac cgccgatatc aactggttta ccctgaaccg
     2461 agattaactc attcacaatt ttttcttcat t
//
LOCUS       DASWBY010000130         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_80090, whole genome shotgun sequence.
ACCESSION   DASWBY010000130 DASWBY010000000
VERSION     DASWBY010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80090"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VF870_02465"
     CDS             <1..875
                     /locus_tag="VF870_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9251071.1"
                     /translation="GVKGGEAVYLDFKDAINRLGQKVIEERYGNLFEIYETITGDNPY
                     KSPMMIYPAPHYTMGGLWVDYNLMSNIPGLFVLGEANFSDHGANRLGASALMQGLADG
                     YFVIPYTMGNYLAGDKPTLVKTDHPEFEKVAKEVKDLTNKLLSIKGKRTVDDLHKQLG
                     RIMWDNVGMGRTEVGLKKTIDQIRQVKEEFWKNVTIPGSGDDVNQTLERAGRLVDYFE
                     LGELMALDALNRNESCGGHFREEYQFEDGEAKRDDENYSYVAAWEFAGENKWNLHKEE
                     LKYEYVKQAIRSYK"
     gene            875..1645
                     /locus_tag="VF870_02470"
     CDS             875..1645
                     /locus_tag="VF870_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX9251072.1"
                     /translation="MEAKKLNLTLNIWRQKNNKSVGSFAEYKVQVSPDASFLEMLDEL
                     NNTLELKGEEAIHFESDCREGICGTCGLVINGHAHGPLPKIATCQLHMRNFKDGETIW
                     VEPFRAKAFPVIKDLMVDRSGFDKILQAGGYVSINTGGVPDGNAIPIQKEISSLAMDA
                     AACIGCGACVASCKNASAMLFVSAKVSQYALLPQGQPERYRRVERMVKVMDELGFGSC
                     TNTYACEAECPKGISVTNIARMNRDFMVAKIKSKEVEV"
     gene            1745..>2369
                     /locus_tag="VF870_02475"
     CDS             1745..>2369
                     /locus_tag="VF870_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251073.1"
                     /translation="MFENEIKFISDFTLNKVKELGSFITIEKLLAIDIHPSIKKYVEG
                     EIDYLISEDRKKLIENSLFDYSGTEISNYFVLIGNEIKKTKKISFEDIKNIVLQAVSF
                     NANYVVRPKWSLSKLIFGNNKSVSVNELKLMLNYTYYYEYLNNVFLAYLSKKKILNIT
                     VTEFELIVNKIDRELFMLHQSKLVDNALFAIADYFSIGGLNKSTVSVE"
BASE COUNT          794 a          361 c          505 g          709 t
ORIGIN      
        1 gaggtgtgaa aggcggagag gcggtttatc ttgatttcaa agatgcaatt aatcgccttg
       61 gacaaaaagt gatagaagaa cgttacggaa acttgttcga aatttatgaa actattacag
      121 gagataatcc gtacaaatcg ccaatgatga tttatccggc tccgcattat acaatgggcg
      181 gcttatgggt agactacaat ttgatgagca acattccggg gttgtttgta cttggtgaag
      241 caaacttctc tgatcacggt gcaaaccgtt tgggtgcttc tgcacttatg cagggattag
      301 ctgatggata ttttgttatt ccttacacaa tgggaaatta tcttgccggc gataaaccga
      361 ctttagtaaa aaccgatcat cctgaatttg aaaaagttgc gaaggaagta aaagatctaa
      421 ccaacaaact tctttctata aaaggcaaga gaactgttga tgatctgcac aaacaacttg
      481 gcagaattat gtgggataat gttggtatgg gcagaactga agtgggattg aaaaaaacta
      541 tcgatcagat aagacaggta aaagaagagt tctggaaaaa tgttacaatc ccgggcagcg
      601 gtgacgatgt taatcaaaca ttagaacgtg caggaagatt ggttgattat tttgaactcg
      661 gcgaattgat ggcgctcgat gcacttaatc gaaatgaatc ttgcggcgga catttccgtg
      721 aagaatatca gtttgaagat ggcgaagcaa aacgtgatga tgaaaattat tcttatgttg
      781 ctgcgtggga gtttgccggt gagaataaat ggaatcttca caaagaagaa ttaaagtacg
      841 agtatgtaaa acaggcaatt aggagttaca aataatggaa gcaaaaaaat taaatctaac
      901 acttaatatc tggagacaga aaaacaataa atctgttgga agttttgctg aatacaaagt
      961 gcaggtttct cctgatgcat cttttcttga aatgcttgat gagttaaata atactctcga
     1021 attaaaagga gaagaagcca ttcattttga aagtgattgc cgtgaaggaa tctgcggaac
     1081 ctgcggatta gtaataaatg gtcacgctca tggtccattg cccaaaattg caacctgtca
     1141 gttacatatg cgcaacttta aagacggcga aacaatatgg gttgaaccat tcagagcaaa
     1201 ggcttttccg gttataaaag atcttatggt tgatcgatca ggatttgata aaatattgca
     1261 ggctggcgga tatgtctcaa taaacacggg cggagttcct gatggtaatg caatcccaat
     1321 tcaaaaagaa atttccagct tagcgatgga cgctgcggct tgtattggat gtggagcttg
     1381 tgttgcttcc tgtaaaaatg cttcagcaat gttattcgtt tcagcaaaag tatcacaata
     1441 tgcactttta cctcaaggtc aacctgagcg ttaccgaaga gttgaaagaa tggttaaggt
     1501 tatggatgag cttggatttg gaagttgtac aaacacctac gcctgtgaag ctgaatgtcc
     1561 taaaggaatt tctgttacca atattgcgag aatgaaccgg gattttatgg tagccaagat
     1621 aaaatccaag gaagttgaag tttagtacaa taattaagtt attttactta atactgatcc
     1681 cgattaactt taatcgggat ttttttatat ttttagccac acaatcaaac aagaagagta
     1741 ttatatgttt gaaaacgaaa taaaattcat aagtgatttt actctgaata aagttaaaga
     1801 gttggggtct ttcataacta ttgaaaaact ccttgcaatc gatattcacc catccataaa
     1861 aaaatatgtg gagggggaaa ttgattatct tatttctgaa gatcggaaga agctgataga
     1921 gaattctctg ttcgattatt cgggtacgga gatctctaac tactttgtgc ttatcggtaa
     1981 tgaaataaaa aaaactaaaa agatctcgtt tgaagatatt aaaaacattg tgcttcaggc
     2041 agtatctttt aatgccaatt atgtagttcg tcccaagtgg tcgctttcta aattgatttt
     2101 tggaaacaat aaatcagttt ctgtaaatga actaaagtta atgctcaatt acacttacta
     2161 ctacgaatat ttaaacaatg tttttcttgc ttacttatca aagaaaaaga ttttgaatat
     2221 tactgttact gaatttgaac tgatagttaa taaaattgat cgcgagttat ttatgctcca
     2281 tcaaagtaag cttgttgata atgcactttt tgcgattgca gattacttca gtattggagg
     2341 tctgaataaa tcaacagtat cagttgaag
//
LOCUS       DASWBY010000131         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_644047, whole genome shotgun sequence.
ACCESSION   DASWBY010000131 DASWBY010000000
VERSION     DASWBY010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644047"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VF870_02480"
     CDS             complement(<1..996)
                     /locus_tag="VF870_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9251074.1"
                     /translation="MEVNTLKPQPSSLLRWSALLLISLAMMGNYYIYDSISPLADLLK
                     TQLGFSDSNIGLLNAIYSFPNIIMVLIGGLLIDRIGTKTSVLIFTALIMMGSIVTAIT
                     GNIWMMSAGRLIFGLGAESMIVAITTIIARWFKGKELSFAFGLNLTVARLGSFLALNS
                     PSWGKGLYEYWQSPLWITVAGGVFAFICVVMYFFLDIYASKNYDLPKEGEQDAILLSD
                     ILRINEKTRWYIFSILAAAVIILSFVNNDNWAIYLISLIVITLVLFLKPKSFSNSFWY
                     ISALCVTFYSAMFPFQTFAIKFFQEAHGTSREVGGNLSSILTLAAMIFTPLFGLLA"
     gene            complement(1176..>2169)
                     /gene="pepD"
                     /locus_tag="VF870_02485"
     CDS             complement(1176..>2169)
                     /gene="pepD"
                     /locus_tag="VF870_02485"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HEX9251075.1"
                     /translation="KKNWLKADILINMDSEELGTIFIGCSGGKNTAATFKAKLEKSPR
                     NYSSFELKVAGLKGGHSGLEIHVGRGNAVKILNRAIWSYTQENKIRLAEINGGNKHNA
                     IPREAFAVVLVPKNDDKKFKKFVAKYNETVKAEFASVEPELVVSIDKHSTPEKVMDEK
                     TQRHLVNALYAIPHGVIKMSNDIPGLVETSTNLAVVETVGKNINIVTSQRSPVYSENV
                     DITNMVTSVFKLAGAEVSYGDGYPGWAPDINSDILKVFKSTFNQMYGKEPEVTAIHAG
                     LECGIIKEKYPDMDMISFGPTMCDVHSPAEKLQISTVPEFYNQLKTVLKNIPAK"
BASE COUNT          685 a          403 c          354 g          727 t
ORIGIN      
        1 tgcaagtaaa ccaaacaatg gggtaaagat cattgcggca agagtcaaaa tgcttgaaag
       61 atttcctcca acttccctgc tggttccgtg tgcctcctga aaaaatttaa ttgcaaatgt
      121 ctgaaacgga aacattgctg aataaaaagt aacgcataaa gctgaaatgt accagaatga
      181 atttgaaaac gatttgggtt ttaagaaaag aacaagcgta ataacaatta aactgataag
      241 gtatatcgcc cagttatcgt tgttgacaaa acttaatata attactgccg cagctagtat
      301 tgaaaatata taccatcgag ttttttcatt tattcttaaa atatcagaaa gtaatattgc
      361 atcctgttca ccttcttttg gcaggtcata attcttgctt gcataaatat ctaaaaagaa
      421 atacataaca acgcaaataa atgcaaacac tccgccagca acagttatcc acaagggcga
      481 ttgccagtat tcatataaac cttttcccca gctgggagaa ttgagtgcaa gaaatgaacc
      541 aagtctggca acagtaagat ttaaaccgaa tgcaaatgat aattcttttc ctttaaacca
      601 gcgcgcaatg atagtggtta ttgcaactat catcgattct gctccaagac cgaaaattaa
      661 tcttccggca gacatcatcc agatattacc tgtaatagct gtaactattg aacccatcat
      721 tataagagca gtaaatatta atacggaagt ttttgtacct atcctatcta tcagtaatcc
      781 gccgatgaga accataataa tatttggaaa cgagtagatt gcatttagaa gtccaatgtt
      841 ggaatcagaa aacccgagtt gagtttttaa taagtctgca agcggactga tgctatcata
      901 aatataatag ttacccatca tcgctaaact aatcagcaat aatgcgctcc atcttaaaag
      961 tgaggatggc tgtggtttta aagtatttac ttccataata tattttaact ttaaattctt
     1021 acacgctgag cctgtttaag cgtgtttgtt tttatttgat tcgccatact tcaacaagct
     1081 cggtatgacg ttgtataata acgatcgcat aataataaaa aaagcagata cttctaaagc
     1141 cgtatctgct tttaatttta taaatcaaga acaaattatt tagccggaat attttttaat
     1201 acagttttta gttgattata aaattccggt accgttgaaa tctgtaattt ttcagcaggt
     1261 gaatgaacat cacacatagt tgggccaaat gaaatcatat ccatatcagg atatttttct
     1321 tttataattc cacattctaa tcctgcatga atagccgtta cttcaggctc tttaccatac
     1381 atctggttga aagttgattt aaaaactttt aggatatcag aattaatatc tggtgcccag
     1441 cctggatagc cgtcgccata ggatacttct gctcctgcta gtttaaatac agatgtaacc
     1501 atatttgtta tatcaacatt ttctgaatac acagggctgc gctggctggt aacaatattt
     1561 atattcttac ctactgtttc cacaactgct aagttagtcg aagtttctac taatccaggg
     1621 atatcatttg acattttgat gacaccgtga ggaattgcat acaaagcatt tactaaatgt
     1681 ctctgcgttt tctcatccat aactttttct ggtgttgagt gtttatcaat cgagaccact
     1741 aattctggtt caacagaagc aaattcagct ttaactgttt cattatactt tgcaacaaat
     1801 ttcttaaatt ttttatcgtc atttttggga acaagtacaa ctgcaaaggc ttctcgagga
     1861 atggcgttgt gtttgtttcc gccattaatt tctgcaagtc ttattttatt ttcttgtgta
     1921 taactccata ttgctctgtt aagaattttt actgcgtttc ctcttccgac atgtatttca
     1981 aggcctgagt gtccgccttt taatcctgcg actttcaatt cgaatgatga ataatttctt
     2041 ggagattttt ctaacttggc tttgaatgta gctgctgtat tttttccacc ggaacatcca
     2101 atgaaaatag ttccaagttc ctcagaatcc atatttataa gtatatctgc ttttagccag
     2161 ttctttttt
//
LOCUS       DASWBY010000132         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_682003, whole genome shotgun sequence.
ACCESSION   DASWBY010000132 DASWBY010000000
VERSION     DASWBY010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682003"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VF870_02490"
     CDS             <1..437
                     /locus_tag="VF870_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251076.1"
                     /translation="NQKGDVQPIGGVNEKIEGFFDICKLNGLTGNQGVIIPIQNIDDL
                     MLRDDIIEAVRKNNFHIYPIKRVEEGIEILTGTKAGKKSAKGYEEGTVFYRVEKKIQE
                     LFEKSRAIRSRNGEEIKNKKLNESIVTSKKNIIKKPERKSKK"
     gene            434..1870
                     /gene="pyk"
                     /locus_tag="VF870_02495"
     CDS             434..1870
                     /gene="pyk"
                     /locus_tag="VF870_02495"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX9251077.1"
                     /translation="MILDADSIAKTKILATLGPATKSVDEIRQLILAGIDGVRLNLSH
                     GTYDFFEDIFNSIHTACLEEKSPLAVLVDVQGPKIRIGELEQPSINLVTGNKVEITIE
                     DIKGNENIISTSYKQLANDAQVGDQILINDGLIRLVVTQKKRKSLICDIVNGGELTPK
                     KGMNLPGMNLSTPSITEKDLKDLEFVLKHRIDYIALSFVRNANDVVELKKWLSEKGKI
                     IPVIAKIEKKEAVDNLHEIILASDGIMIARGDLGVELPPQEVPVLQKSIIRQCNALGK
                     LVITATQMLESMINSPIPTRAEASDVANAVWDGTDVVMLSAETSVGKFPFRTVQIMND
                     IIKNAEQHNEDKRNNNYITPDALQEKLFDSVGKAIVEISKQINAQAIVVFTFEGRTAR
                     IISKYNPDSKIIAISNSFSTMNNLCLRRGVTSVYREAINKEHLAIDDAKELILSSGLA
                     KEGDLIIFTAGAPYSEKSRANWLRFEVI"
     gene            1867..2898
                     /gene="amrS"
                     /locus_tag="VF870_02500"
     CDS             1867..2898
                     /gene="amrS"
                     /locus_tag="VF870_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX9251078.1"
                     /translation="MKMINQGMTEALWWHKTDRGKIMCTLCPRYCEIGEGQPGFCYIR
                     QNIDGKLYSLGYGKPTGFGIDPIEKKPLNHFLPGSTVLSFGTAGCNLGCKFCQNWSIS
                     KAKLDEVNSLTASAEDVVMLAKKYSTPSIAFTYNDPTIFGEYVIDISKIAKEEKIKSV
                     MVTAGYIDKQARKDVYKFIDAANLDLKGFTERFYYKNTFSHLEDVLGTMIWLKNETDV
                     WIELTTLLIPNENDSDEEIKNECDWILENLGENVPLHFTAFHPDFKMINKSPTPHSTL
                     LRAKKIAGNLGIKYCYVGNVNDTKNQSTYCTGCGSLLIERNWHQIKIVNLEKDKCRKC
                     GNVVAGIYS"
     gene            2918..>3427
                     /locus_tag="VF870_02505"
     CDS             2918..>3427
                     /locus_tag="VF870_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9251079.1"
                     /translation="MLNLEGKIVLITGASSGIGQACAREFAKLKTNLILAARREDRLK
                     VLANELENEHKIKVKYLEVDVRNFDDVQKKFNSLESSWRQINILVNNAGLAKGLDKIF
                     EGKLSDWDEMIDTNLKGLLNVTRVVSPGMVERQSGHIINIGSTAGHEVYTYGNVYSAT
                     KFAVKSLTQS"
BASE COUNT         1265 a          488 c          663 g         1011 t
ORIGIN      
        1 taaatcaaaa aggtgatgtt cagccaattg gcggtgttaa cgaaaagata gaaggctttt
       61 ttgatatttg taagttgaat ggattaacag gaaatcaagg ggtaataatt cctatccaga
      121 atattgatga tcttatgctt cgtgatgata ttatcgaagc cgtaagaaaa aacaattttc
      181 atatatatcc aataaaacga gttgaagaag ggattgaaat tcttactggt acaaaggctg
      241 gaaaaaaatc agcaaaaggt tacgaagaag ggactgtttt ttatagagtt gaaaagaaaa
      301 tacaagagct ctttgaaaaa tctcgtgcaa ttagatcaag aaatggcgaa gaaataaaaa
      361 acaaaaaact taatgaaagc attgtgacat caaaaaaaaa tattattaaa aagcctgaaa
      421 gaaaaagtaa aaaatgattt tagatgctga ttcaatcgcc aaaacaaaaa tactcgctac
      481 acttggtcct gcaaccaaaa gtgttgatga aataagacaa ttaattttag caggcatcga
      541 tggcgtccga ttaaatttat cacacggtac atatgatttt tttgaagata tttttaattc
      601 tattcatact gcctgccttg aggaaaaatc tccactagct gttttagtag atgttcaggg
      661 accaaaaatt agaattgggg aattagaaca accgagcata aatcttgtta caggaaataa
      721 agttgaaata actatagaag atattaaagg taatgaaaac attatttcaa catcttataa
      781 gcaattggct aatgatgcac aagttggtga ccagatacta attaacgatg gtttgatacg
      841 ccttgtggtt acgcagaaaa aacgtaaatc actgatttgt gatattgtga atggcggaga
      901 attaacgcca aaaaaaggta tgaatctacc aggaatgaat ttatcaactc cttctatcac
      961 agaaaaagat cttaaggatc tggaatttgt tttaaagcat cgtattgatt acatcgccct
     1021 gtcgtttgta agaaatgcta atgatgttgt tgaactaaaa aaatggcttt ctgaaaaagg
     1081 aaagatcatt cctgttattg ccaagattga aaagaaagaa gcagttgata atcttcatga
     1141 aataatttta gcctcagatg gaattatgat tgcccgtgga gatttaggtg ttgagctgcc
     1201 accacaagaa gtacctgttt tacagaaatc aattattagg cagtgcaatg cattaggtaa
     1261 attagtgata actgcaactc agatgctgga atcaatgatc aactcaccta ttccaacacg
     1321 agctgaggct tctgatgttg caaacgcagt ttgggatgga actgatgttg ttatgctgag
     1381 tgcagaaact tcggttggaa aattcccttt tcgcacagtt cagatcatga atgatattat
     1441 taaaaatgct gaacagcata acgaggataa aagaaataac aattatataa ctccggatgc
     1501 tcttcaggaa aaattatttg attcggttgg caaagcaatt gttgagatca gtaaacaaat
     1561 taatgctcaa gctattgttg tttttacgtt tgaaggaaga actgcaagaa ttatttctaa
     1621 gtataacccc gattcaaaaa taatagcaat ctcaaacagc ttttcaacaa tgaataatct
     1681 ttgtttacgt cgtggtgtaa catctgttta cagagaagct attaacaaag aacatctcgc
     1741 aatcgatgat gctaaagaat taatacttag ttctggtctt gcaaaagaag gtgatttgat
     1801 tatctttaca gcaggagcac cttattcaga gaaaagcaga gctaattggt taagatttga
     1861 agtgatatga aaatgatcaa tcagggaatg acagaagcac tgtggtggca caaaactgat
     1921 cgtggaaaaa taatgtgcac attgtgtccg cgctattgtg aaattggtga aggccaacct
     1981 ggtttttgtt acatcagaca aaacattgat gggaaacttt attctttagg ttatggaaaa
     2041 ccaaccggat ttggaattga tccgattgaa aaaaaaccac taaatcattt tttgcccggt
     2101 agtacagttt taagctttgg tactgcaggc tgcaaccttg gatgtaagtt ttgtcaaaat
     2161 tggtcaatca gcaaggcaaa gttagatgaa gttaattcct taacggcatc agctgaagat
     2221 gttgttatgc tggcaaagaa atattctaca ccatcaattg ctttcacata taatgatcca
     2281 acaatttttg gtgagtatgt aatagatatc tccaaaattg caaaagaaga aaagattaaa
     2341 tccgtaatgg ttactgctgg atatattgat aaacaagcgc gaaaagatgt ttataaattt
     2401 attgatgctg ctaaccttga tttaaaagga tttacagaaa gattttatta caaaaatact
     2461 ttttcacatt tagaagatgt attgggtaca atgatttggc taaaaaatga aactgatgtt
     2521 tggatagaac ttactacact cctgattccg aacgaaaatg attctgatga agaaataaaa
     2581 aatgagtgcg attggatctt agaaaatctt ggagaaaatg ttcctcttca ctttacagcg
     2641 tttcatccgg attttaaaat gataaataaa tcaccaactc ctcattcaac tttattacgt
     2701 gcaaaaaaga ttgccggtaa tcttggaatt aaatattgtt atgtgggaaa tgttaatgac
     2761 acaaaaaatc aatctactta ctgtaccgga tgtggaagtt tattgatcga aagaaactgg
     2821 catcaaataa aaattgttaa tttagaaaaa gacaaatgca gaaaatgtgg gaacgttgtc
     2881 gccggaatat attcataata attattaagg ataatcaatg ttaaatcttg aaggtaaaat
     2941 cgttttaata actggtgcat catctggaat tggacaagct tgtgcaagag aatttgctaa
     3001 acttaagacc aatttaatat tagcagctcg aagagaagac cgattaaaag tattggctaa
     3061 tgaattagaa aatgagcata agataaaggt taaataccta gaggttgatg ttagaaattt
     3121 tgatgatgta caaaaaaaat ttaattcttt ggaatcatcc tggagacaaa ttaatatatt
     3181 agtaaacaac gccggcttag ccaaaggctt ggataaaatt tttgaaggaa aattatccga
     3241 ctgggatgaa atgatcgata caaatcttaa ggggttgctg aatgttacaa gagtggtatc
     3301 tccaggtatg gtagaaagac aaagcggaca tattatcaac attggctcta ctgcaggaca
     3361 tgaagtctat acttatggca acgtgtattc cgcaactaaa tttgctgtta aatctttaac
     3421 ccaatct
//
LOCUS       DASWBY010000133         5882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_682062, whole genome shotgun sequence.
ACCESSION   DASWBY010000133 DASWBY010000000
VERSION     DASWBY010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682062"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..660
                     /gene="crcB"
                     /locus_tag="VF870_02510"
     CDS             283..660
                     /gene="crcB"
                     /locus_tag="VF870_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX9251080.1"
                     /translation="MLNYILVSAGAAIGGALRYGISSYIQKNISVIFPYGTLVVNVIG
                     SFLIGIIMFYLNEKELIGNEFRLFLTIGFCGGFTTFSTFSYETLNLFRDSEYMLAFYN
                     VILNVVLCFVGIYLAYLISKLIG"
     gene            664..1017
                     /locus_tag="VF870_02515"
     CDS             664..1017
                     /locus_tag="VF870_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX9251081.1"
                     /translation="MQTNSEAKLLRIFIGESDKLHGKSVYEEIVLKAREANLAGATVI
                     KGIMGFGANSKIHTSKVLTLSEDLPLIVEIVDTLEQIEKFIPVVNELFEKANIGGLVT
                     IEKAEVIKYSANKIK"
     gene            complement(1033..1815)
                     /locus_tag="VF870_02520"
     CDS             complement(1033..1815)
                     /locus_tag="VF870_02520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX9251082.1"
                     /translation="MSTAEITSTSLIILAALIFVMLERIFPYTKGQEILREGFFDDFA
                     MYTIAQNYVLSIIIFSGIIHFIDNTTGLSRLQLFADIPIWAQLIFFVVTHDLYIYWMH
                     RWMHKNKWLWRIHEAHHSPKKVDWLSGSRSHPLEILLNQTIEFAPIILLGAPVEVIAY
                     KGLISAIWGMYIHCNVDIQSGWLQKIINGPEMHRWHHSTGKGRNRNFATKFAFWDWIF
                     GTAFLPETKPDDYGLKTFFPAHYLKQTLFAFRSFKKSKSSSE"
     gene            complement(1987..2865)
                     /locus_tag="VF870_02525"
     CDS             complement(1987..2865)
                     /locus_tag="VF870_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEX9251083.1"
                     /translation="MSKREIKVADRFIGDGHPVFVIGEIGINHNGDIRIAKKIIDGAL
                     HAGCDAVKFQKRTPEICTPKDQWNIERDTPWGRMTYIDYRHKVEFGEQEYAEIDKYCK
                     KNGMLWFASCWDEPSVDFIEQFNPPLYKTASAVLTDFPLLDKHKHLNKPIMMSTGMST
                     MEEINKAISFMDKNNLMIAHATSAYPCKIEELNLKMIETLKKKYPDIPIGYSGHEVGL
                     APTWAAVALGACFVERHITLDRAMWGTDQAASVEVGGMERLVSSIRDIEKSLGDGVKK
                     VYDSELGQIKKLRRVK"
     gene            complement(2883..3413)
                     /locus_tag="VF870_02530"
     CDS             complement(2883..3413)
                     /locus_tag="VF870_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX9251084.1"
                     /translation="MVIDTKLRDKNLHLKEKAEKIKVILTDVDGVLTDTGIYYGKDGE
                     AFKRFSIRDGMGVERLRKYANIETIIITGENSNTVKTRAEKLKMKEFYLGVNKKEDVL
                     ESIKKKNGFEKENIAYIGDDANDFDVMQLCGFTAVPNDGMNFIKDIADYVCETSAGYG
                     AFREFAELILAFKNNS"
     gene            complement(3407..4219)
                     /locus_tag="VF870_02535"
     CDS             complement(3407..4219)
                     /locus_tag="VF870_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9251085.1"
                     /translation="MNSLDLFSLKNKVAIVTGALGLIGKNHCAALADAGANIVVCDLD
                     EVKCETFASTLSTKSIGVGVDITNKSSVENLKDRCIKEFGRIDILVNNAAINDMFENP
                     QAAAEQSMFENYPLDMWQKSLDVNVTGTFICSQIIGSEMAKAGKGSIINVASTYGLVG
                     PDQSIYKKPDGSQSFYKSPAYPATKGAVVNFTRFLAAYWGNKGVRVNTLTPGGVENNQ
                     DEYFVNNYSAKTPLGRMAYPTDYKGAIIYLASDASSYMTGANLIVDGGWTAW"
     gene            complement(4315..5649)
                     /locus_tag="VF870_02540"
     CDS             complement(4315..5649)
                     /locus_tag="VF870_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9251086.1"
                     /translation="MPNKVDLNIDYPSIKKSDEYYKIALDLIPCTTQTLAKGPQQNVK
                     GVAPKYLRRGKGSHVWDVDGNEYIDYLMAIGPLSLGYAYDKVDEAIKEQLKDGITFSL
                     MHPLEVEVAELIHKVVPNAESIRYSKVGADVTSAAVRLARAFTKRDKVLCCGYHGWHD
                     WYIAVTDRNKGIPQSIQDMTYTINYNDIQSVIDSVDEDTACVILEPYVFEEPKDNFLQ
                     KLRDVCTKNGTLLIFDEMWSGFRISIGGAQEYFGVNADLACFSKAVANGMPISILTGR
                     KDVMSLLEQEVFFFTTFGGEALSLAAVKATITEMIEKNVPAQLARQGNKLKVGYNKIA
                     EALGMSYTKCSGFDCRTIISFDASAGNPLEMKSLVQQEMIKRGILWGGFHNMSFSHSN
                     ADIEYTLKVYKEVLPILKKAVEENNVKGYLRGEPVGPVFRKVSNFNMKPKKK"
     gene            complement(5700..>5882)
                     /locus_tag="VF870_02545"
     CDS             complement(5700..>5882)
                     /locus_tag="VF870_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HEX9251087.1"
                     /translation="KRVYDELYQINNKFTLKDILDLLDRKPDIKKINEEYCGVNWYRN
                     HLNELKTISSNQTKII"
BASE COUNT         1795 a         1099 c          933 g         2055 t
ORIGIN      
        1 taaatgccgg actcggaatt agttctggtg aaggtgactt ggaatttaat gtaacttatt
       61 cccgcagctg gtgggatgaa cctggagaag gtttcggtac aaacgttcct cctgttgatc
      121 agtcaattcg tatagataat attatgacat catttacagt tcggttttaa atcgactatt
      181 tttaattttt tataaataaa taatttcaaa gtctcatagt taattctgtg ggactttttt
      241 attttaaatc atagtttatt taagaaacct aaaaaatcaa aaatgcttaa ttacattctt
      301 gtatcagcag gtgccgctat tggcggtgcg cttcgttatg gtatctccag ttacatccaa
      361 aaaaacattt cagtaatctt tccttacggt actttagtgg taaacgttat tggttctttt
      421 ttaataggta ttattatgtt ttatttaaat gaaaaagaat taattggaaa tgaattcagg
      481 ttatttctta cgataggttt ttgtggcgga tttactacct tctcaacatt ctcttacgaa
      541 acgctaaatt tgtttcgtga ttcagagtat atgctggctt tttacaatgt tattctaaat
      601 gtagttcttt gttttgttgg gatatatctg gcttatttaa tttcaaaact tataggttga
      661 tttatgcaaa caaatagtga agctaaactg ctaagaatat ttataggtga gagtgataaa
      721 ttacacggca aatcagttta tgaagaaatt gttttgaaag cacgggaagc aaatttagca
      781 ggtgcaactg ttataaaagg aataatgggt tttggtgcaa acagcaaaat tcatacatca
      841 aaagttctaa cactttctga agacttacca ttaattgttg aaatagttga tacgcttgaa
      901 caaattgaaa aatttattcc tgttgtaaat gaactatttg aaaaagcaaa tattggtggt
      961 cttgttacga tagaaaaagc tgaggtgata aaatactctg ctaataaaat aaaatagagt
     1021 aaaaataaat aatcattcag aactgctttt tgatttttta aaactcctga aagcgaataa
     1081 agtctgcttg agataatgag cagggaagaa agtctttaat ccgtaatcat caggttttgt
     1141 ctccggtaga aatgccgtcc caaaaatcca atcccaaaaa gcaaatttag tcgcaaagtt
     1201 tctgtttctt cctttacctg tagagtgatg ccagcgatgc atttcaggtc cattaattat
     1261 tttttgcagc cagccggatt gaatatcaac attacaatga atatacattc cccaaattgc
     1321 acttatcaga cctttgtatg caattacctc aactggagca ccgagtaaaa tgattggtgc
     1381 aaattctatt gtctgattta aaagtatctc cagcggatgc gatcttgaac ctgataacca
     1441 atcaactttt tttggcgaat ggtgcgcttc gtgtattctc cacagccatt tatttttatg
     1501 catccagcga tgcatccagt aaatgtaaag atcgtgggtt acgacaaaga aaattaattg
     1561 tgcccagatt ggaatatcag caaaaagctg caggcgtgat aaacccgtag tattatctat
     1621 aaaatgaatt atcccgctga agatgattat gcttaaaaca taattttgag caatcgtata
     1681 catcgcaaaa tcatcaaaga aaccttcacg aagaatttcc tgaccttttg tataaggaaa
     1741 tattcgttcc agcattacaa aaattaacgc tgcaagaatt atcaacgaag ttgatgttat
     1801 ttccgcagtg gacatagtgc ttatataatt ataaatgcta tttaatatat ctgtaaaaat
     1861 gtttgcttct gacatcaaaa taaattttta ttgcgctgat gtcatcctga gcgaagtcga
     1921 aggatgacgg tttgtggata attccagatt agtcacactt cgtcttcgct cagtgtgact
     1981 aaaatatcat tttactcttc ttagtttttt aatctgcccg agctcgctat cataaacttt
     2041 tttgacaccg tctcctaaag atttttcaat atcacgaata ctggaaacta atctttccat
     2101 tccaccaacc tccacagagg cagcctgatc agtaccccac attgcacgat ctaaagttat
     2161 gtgtctctct acaaaacaag caccaagcgc aactgcagcc caggttggtg ctaatcccac
     2221 ttcatgtccg ctgtaaccaa tcgggatatc gggatatttt ttctttagtg tttcaatcat
     2281 cttaagattt aattcttcaa tcttacaagg atatgctgaa gtagcgtgag caatcattaa
     2341 attattttta tccataaaag aaattgcttt attaatttct tccatggttg acattcctgt
     2401 agacatcata attggtttat tgagatgttt atgcttatcc aataaaggaa aatcagttaa
     2461 taccgctgag gctgttttgt aaagaggagg attaaattgt tcaataaaat caactgaggg
     2521 ttcatcccaa caggaggcaa accaaagcat tccatttttc ttgcagtact tatcaatttc
     2581 tgcatattcc tgttcgccaa attctacttt atgtctgtaa tcaatgtagg tcattctacc
     2641 ccacggagtg tcacgttcta tattccattg gtcttttgga gtacagattt ccggagttcg
     2701 tttttgaaat ttaacagcat cacaacctgc gtgcaaggcg ccatcaataa ttttttttgc
     2761 aattctaata tcgccgttat gattaatgcc gatttctccg attacaaata ccggatgacc
     2821 atcgccaatg aatctatcag cgactttaat ttctctttta ctcataaaaa tctttctgta
     2881 atctaagaat tattcttaaa ggctaaaatc aattcggcaa actcacgaaa agcaccataa
     2941 cccgcacttg tttcacaaac ataatcggca atgtctttta taaaattcat tccatcattt
     3001 ggtacggcag taaatccgca aagctgcata acatcaaagt catttgcatc atcaccgata
     3061 taggcaatgt tttctttttc gaagccattt ttctttttaa tgctttccag gacatcttct
     3121 tttttattta cgccaagata aaactccttc atttttaatt tttcagcacg agttttaaca
     3181 gtattggaat tttctccggt gataattata gtttcaatat tcgcatattt tcgtaatctc
     3241 tcaacgccca ttccatcccg aatagaaaat cttttaaatg cttcaccatc tttaccataa
     3301 tagattccgg tatcagttaa aacaccgtca acatctgtca gtataacttt tatcttttct
     3361 gctttttctt ttaggtgaag atttttatct cttaactttg tatctattac catgctgtcc
     3421 atcctccatc aacgattaaa tttgctcctg tcatataaga tgaagcatca cttgctaaat
     3481 atattattgc acctttgtaa tccgttggat aggccattct gcccaaaggt gttttagcag
     3541 aataattatt tacaaaatat tcatcctgat tattttcaac tccaccgggt gtaagtgtat
     3601 taaccctaac tcctttgtta ccccaatatg ctgcaagaaa tcttgtaaaa ttaactacag
     3661 cgcctttcgt tgcaggatag gcaggtgatt tataaaatga ttgtgatccg tcaggttttt
     3721 tatagattga ttgatcaggt ccgactaatc cgtaagttga tgcaacatta atgatactgc
     3781 ctttgccggc tttagccatt tcagatccga ttatttgtga acaaataaaa gttcctgtta
     3841 catttacatc taaagatttt tgccacatat caagtggata attttcaaac atagattgtt
     3901 cagccgcagc ttgtggattt tcaaacatat catttatagc agcgttgttt actaaaatat
     3961 caatcctccc aaattccttt atgcatctat ctttaagatt ttcaacggaa gatttattag
     4021 taatatctac accaactccg attgattttg ttgatagggt agaagcaaat gtttcgcatt
     4081 ttacttcatc aagatcacac acaacaatat ttgctccggc atcagcaagt gcggcacaat
     4141 gattttttcc gatcaaacca agtgcacctg ttactattgc tactttgttt tttagcgaga
     4201 atagatctaa tgagttcatt tcttttttct ttgtcaagct gaacttgttt cagcttcttt
     4261 agttttctca agtacaatct agagattccg aaacaagttc ggaatgacta atgcttattt
     4321 cttcttcggt ttcatattaa agttactaac ttttctgaac accgggccga caggttcacc
     4381 gcgcaaatat ccttttacat tattttcttc aacagctttt tttagtatcg gaagaacttc
     4441 tttgtaaacc tttaatgtat attcaatatc agcattagag tgtgaaaaag acatattatg
     4501 aaatccaccc cagagtattc cgcgttttat catctcctgc tggacaagtg atttcatttc
     4561 aagtggattt ccggcagatg catcaaatga tattattgta cggcaatcaa aacctgaaca
     4621 ctttgtgtaa ctcattccga gtgcttcagc aattttatta taaccaactt ttaatttatt
     4681 gccttgtctt gcaagttgtg caggaacatt tttttctatc atttcggtga ttgtagcttt
     4741 aactgctgcc agagaaagtg cttcaccgcc aaaggttgta aagaaaaaaa cttcctgctc
     4801 aagtaaagac attacatctt ttcttccggt caaaattgaa atcggcattc cattagccac
     4861 tgcttttgaa aagcaggcaa gatcagcatt tactccaaaa tattcctgtg cacccccgat
     4921 tgaaattcta aaaccgctcc acatttcatc aaagattaat agtgttccat tctttgtgca
     4981 gacatcacgc aatttttgta aaaaattatc tttgggttcc tcgaaaacat atggttcaag
     5041 aatcacacaa gctgtgtctt catcaacgga atcaataaca gattgaatgt cattgtaatt
     5101 aattgtgtaa gtcatatcct gaatcgattg tggaattccc ttattgcgat ctgtaactgc
     5161 tatgtaccaa tcgtgccagc catgatagcc gcagcataaa actttatctc gttttgtaaa
     5221 tgcacgggct aatcttacag ctgcggatgt aacatctgca ccaactttac tgtaacgaat
     5281 tgattctgca tttggaacaa ctttatgaat caattctgca acttcaactt ccaatggatg
     5341 cattaaggaa aaggtaatac cgtcttttaa ttgctctttg atggcttcat caactttatc
     5401 ataagcataa ccaagcgata gaggacctat tgccattaaa taatcgatgt attcatttcc
     5461 atcaacatcc caaacatgtg aacccttgcc tcgccgcaga tattttgggg caacaccttt
     5521 tacattttgc tgtggacctt ttgcaagtgt ttgagtggtg catggaatta aatccaatgc
     5581 aattttataa tattcatcag atttttttat cgatggataa tcaatattta aatcaacttt
     5641 attaggcata attaaacttt cttataaaaa tccgcctaag cgaatatttt ctattatatt
     5701 taaattattt ttgtttggtt tgatgatatt gtttttagtt cattaagatg atttctgtac
     5761 caattcactc cgcaatactc ttcatttatt tttttaatgt caggttttct atccaacaaa
     5821 tcaagaatat cttttaatgt aaatttatta tttatttgat aaagttcatc atatacccgt
     5881 tt
//
LOCUS       DASWBY010000134         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_606219, whole genome shotgun sequence.
ACCESSION   DASWBY010000134 DASWBY010000000
VERSION     DASWBY010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606219"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..648)
                     /locus_tag="VF870_02550"
     CDS             complement(187..648)
                     /locus_tag="VF870_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251088.1"
                     /translation="MRTKHLKALVFILAVILTSMSCSKNDEKVNISAAGLKNSHKMGK
                     NCMECHKSGGWGKGAFVVAGTVYNNGLSNTYADAVVKLYTQASGGGTLAGTIYGDANG
                     NFYTTESIDLSGGVYPSVSGTGGSVNYMQSSITVGACNSCHGNSVAKLWAK"
     gene            complement(900..1460)
                     /locus_tag="VF870_02555"
     CDS             complement(900..1460)
                     /locus_tag="VF870_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9251089.1"
                     /translation="MKLYIKNMVCDRCKMVVKTEFEKIGIHPLSVNLGEVDLNKDLNE
                     SEKLELKDKLQQLGFFLIDNKRSHLIGRIKTLIIELVHQQNSELKTNLSDFLSSHLNH
                     DYNYISTLYSEVEGTTIEKYFIAQKIERVKELLIYDEFSLSEIANLLNYSSVAYLSNQ
                     FKKVTGLTPSYFKSIKINRRIPLDQV"
BASE COUNT          610 a          362 c          301 g          765 t
ORIGIN      
        1 caagaaaaag gtcaaaggtg atttactttt tgtcgtacta ttaaacagat gtgtgttcat
       61 gaacttcctt ttttattaat taattaactt ttcatttgaa gggcttatca tcctgtgttt
      121 tgttttgaat tcatcacttt aatctcttta ttgttagact ctttctaatg aaagaaaagc
      181 taaagatcat ttcgcccaca atttagcaac agaatttccg tgacaactgt tacatgcacc
      241 tactgtaatt gaagattgca tataatttac tgaaccacct gtgccgctga cagatggata
      301 aactccgccg ctcaaatcaa tggattctgt ggtatagaaa ttgccattag cgtctccata
      361 aatggttcct gcaagcgtcc caccacctga tgcttgcgta taaagcttaa ctacagcatc
      421 agcataagta ttactgagcc cgttattata aactgttccg gcaacaacaa atgcaccttt
      481 accccaccca ccggatttgt ggcattccat gcaatttttt cccattttat gactattttt
      541 caatccggct gcactgatgt tcactttctc gtcatttttt gagcatgaca ttgatgtcag
      601 aataactgca aggataaaaa ccagagcttt taaatgtttc gttctcataa ttaaaatttt
      661 tacatttaat taaagatccg ttttagaatt aataatgtct atttgtatcc taaacctgga
      721 tccgataaaa tcagtctgta taaaatcata ccccataata ctcacttttg cctccttcta
      781 atgaaatatg aattttgtag tctttttctt tagccatatt cacaacaact aattacacta
      841 caaaattcgt cacaatacag aatcctgtgt tacaagattt ctttgaagag ttgtaatatt
      901 tacacctgat ctagtggtat acgtctgttg attttgatgc tcttaaagta gcttggtgta
      961 agtccggtta cttttttaaa ctggttgctc aaataggcta cacttgaata gttgagtaaa
     1021 tttgcaattt cactcaatga aaattcatca tatattaaaa gctctttgac tctttcaatt
     1081 ttttgagcta tgaaatactt ttcaatcgtt gttccctcaa cttctgaata taaagtgctt
     1141 atataattgt aatcgtgatt taaatgactg cttaaaaaat cggaaaggtt tgttttcaat
     1201 tcactgttct gttgatgtac taactctatt attagagttt tgattctccc gatcaaatgg
     1261 ctccttttgt tgtcgatgag gaaaaaacct aattgttgta atttgtcttt taattctagt
     1321 ttttctgatt cattcaggtc tttgtttaag tcaacctcac ctaagttaac cgaaagagga
     1381 tgaattccaa ttttttcaaa ttcggttttc accaccatct tgcaacggtc acataccata
     1441 ttttttatat aaagtttcat ctattgtttt ctgcttttgt tcaaagatat gttgatttat
     1501 ttcctcaaat taacttgatg atatttagca ctattgcctg tataaggatg agttgtccgc
     1561 tttggattga atcacattga atttcttccc tgatattatg atgtaatgat gtacaggtat
     1621 tggattcagt agctcgccta tgtaaagaat tatttactct atttggtcag ggtaaggact
     1681 ctcacttaat cttgattttt cagcattaac ctttcaataa atttaaagtt ggaaccacaa
     1741 agcttttcaa tggctcagct ttagataaat cattatccat ttacctatgg gattttattt
     1801 tcagaagggt gttacaaatt cgtcgtgatc aataaaatcc caaggaattt cttttttaaa
     1861 atttgaatac attcttccat tagtgaaagt tcgatctctt tctaaataag gatattattc
     1921 tagtttgtca atcaaccatt attggcaaag aagtatgaca ataactgtaa attaaaattt
     1981 tgatggatta ctattttcac gctttcaaca aaactgtcaa tataatgcat tgtttgaa
//
LOCUS       DASWBY010000135         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_418721, whole genome shotgun sequence.
ACCESSION   DASWBY010000135 DASWBY010000000
VERSION     DASWBY010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418721"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /gene="pgl"
                     /locus_tag="VF870_02560"
     CDS             complement(<1..667)
                     /gene="pgl"
                     /locus_tag="VF870_02560"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX9251090.1"
                     /translation="MNYKIQISETVEELSEQFAQLLIDKVNNSKDKFNIALSGGSTPK
                     NIFEYLVAHHHDTIEWHKVNFFWGDERCVPPNDSESNYKMAYEAILSRLQIPDSNIFR
                     IRGENDPQNEAKEYSEIVVKQLPQINNIPKFDLIMLGLGEDGHTASLFPNQKSLLESE
                     KIYATAVHPETGQKRITLTGRVINNALTIVFIVTGINKAKVVNEIINQKENYKYYPAS
                     SIKS"
     gene            complement(657..2174)
                     /gene="zwf"
                     /locus_tag="VF870_02565"
     CDS             complement(657..2174)
                     /gene="zwf"
                     /locus_tag="VF870_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX9251091.1"
                     /translation="MNDNNHIYVIFGASGDLTKRKLVPAIYSLFVQNMLPENFVLLGA
                     SRTEYSDDDFRKYMKSSIKEFKEIDDDTKIDDFIKKIFYTSISFDDTAAYTKLSDRIS
                     ELRKDNNIGGNAIFYLSTPPSLYGTIPQHLAKVGLNKQNDGWKRLIIEKPFGYDLQSA
                     QKLKDELLRDWEEEQIFRIDHYLGKETVQNLLVTRFSNGIFEPLWNRNYIHHVEVTSA
                     ESIGVEKRGGYYESSGALRDMVQNHLLQVVGLTAMESPSSLEPIAIRNEILKVFQSLR
                     PIKNEDVKKVAIRGQYMSSKIKGEGVLGYREEEGVNPESVTETYAALKFYIDNWRWGG
                     VPFYVRTGKRLPTRVTEVVIHFKPTPHFLFAKDEMHNACNQLIVRIQPDEGILLKFGM
                     KTPGAGFDVQNVNMDFHYSDLSNQRIPSAYERLLHDTMKGDATLFARTEEVLEAWKFL
                     TPVIECWKDDKNVPLFGYPAGTWGPENADDLIEDKNMGWRYPCKNLADDGVYCEL"
     gene            complement(2378..>3058)
                     /gene="gnd"
                     /locus_tag="VF870_02570"
     CDS             complement(2378..>3058)
                     /gene="gnd"
                     /locus_tag="VF870_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX9251092.1"
                     /translation="VDENIDSLQKLLKEDDIIIDGGNSYWKDTQKRSEKLAKYRIHYL
                     DCGTSGGVWGLQNGYCLMYGGEKNATDFVEPIFKSLAPENGYVYCGQSGTGHLVKMVH
                     NGIEYGMMQAYAEGFEILEKSPYKIDITKVADAWQYGSVVRSWLLELAVNAFKDDPKL
                     EKLKDYVQDSGEGRWTVQTAMDFDVPAHVITASLYTRFQSRQDESFAMKVLAALRNQF
                     GGHAVKIK"
BASE COUNT          890 a          634 c          470 g         1064 t
ORIGIN      
        1 gcgatttaat agatgaagca ggataatatt tataattctc tttttgattt atgatttcat
       61 taacaacttt agctttatta attccggtta caataaacac aatggttaaa gcattattta
      121 ttactcttcc tgttaatgta attctctttt gtcctgtttc aggatgcaca gcagttgcat
      181 aaattttttc tgattctaat aaactttttt ggtttggaaa taatgatgct gtatggccgt
      241 cctcaccaag tcccagcatt attaaatcaa atttagggat attgtttatt tgcggtagct
      301 gctttactac tatctctgaa tattccttcg cttcattctg aggatcattt tcacctctaa
      361 ttctaaaaat atttgaatca ggaatttgca gtctgctcaa tattgcttca taagccattt
      421 tataattgct ttcagaatca ttaggaggaa cacagcgttc atcgccccaa aagaaattta
      481 ctttgtgcca ttcaattgtg tcgtgatgat gtgccactaa atattcaaat atatttttgg
      541 gtgttgatcc gcctgataat gcaatattga acttatcttt actgttattt actttatcaa
      601 taagaagctg tgcaaattgc tcagataatt cttcaacagt ttcgcttatc tgaattttat
      661 aattcacaat aaactccatc atcagccaaa tttttgcagg gataacgcca tcccatattt
      721 ttatcctcaa tcaagtcatc tgcgttttcc ggtccccatg ttcctgcagg ataaccaaac
      781 aaaggaacat ttttatcatc tttccagcat tcaattacag gagtcaaaaa tttccaggct
      841 tctaaaactt cttcggtacg tgcaaacaaa gttgcatcac ctttcatagt atcgtggagt
      901 aatctttcat aagcagatgg tattctttga tttgaaagat cagaataatg aaaatccata
      961 ttcacatttt gaacatcaaa gcctgcgccc ggagttttca ttccaaactt taataaaatt
     1021 ccttcatccg gttgaattct gactatcaac tgattacaag cattatgcat ttcatcttta
     1081 gcaaagagaa agtgcggtgt tggtttaaag tgaattacaa cttcagtaac tcgcgtaggc
     1141 aatcgtttac ctgtgcgaac ataaaacggc acgccgcccc agcgccagtt atcaatataa
     1201 aactttaatg ccgcatacgt ctctgtcaca gattccggat taacgccttc ttcttcacga
     1261 tatccaagaa caccttcgcc cttgattttt gatgacatat attgtccgcg aatagcaact
     1321 ttttttacat cttcattctt aatcgggcgc aatgactgaa atactttcaa aatttcatta
     1381 cggatagcaa ttggttcaag ggatgaaggc gattccattg cagttaatcc aacaacctgt
     1441 agtaaatgat tctgcaccat atcacgcaaa gcaccggatg attcatagta cccgccgcgc
     1501 ttttctacac cgatactttc tgcggatgtc acttcaacat gatgaatgta gttcctattc
     1561 caaagaggtt caaatattcc gtttgaaaat ctggtaacca aaagattctg aactgtttct
     1621 tttccgagat aatgatctat tctaaatatt tgttcttctt cccaatctct cagaagctcg
     1681 tctttaagtt tttgtgctga ttgtaaatca tagccgaatg gtttttcaat tattaaacgc
     1741 ttccaaccat cattctgttt gtttaatcca actttagcaa gatgttgagg aattgttcca
     1801 tataaacttg gcggagtcga taaataaaat attgcgttgc cgccaatgtt attatctttt
     1861 ctaagctcgc ttattctgtc gcttaattta gtatatgcgg ctgtgtcatc gaaacttata
     1921 gaagtgtaaa atattttttt tataaaatca tcgatctttg tatcgtcatc aatttcttta
     1981 aactctttta ttgaagattt catatatttt ctaaaatcat catcgctata ttctgtacgt
     2041 gatgcaccca gcaaaacaaa attctcaggc agcatatttt gaacaaagag agaataaata
     2101 gccgggacta atttcctttt tgtcagatca cccgatgcgc cgaatattac ataaatgtga
     2161 ttgttgtcgt tcatattatt cctttctttt ttcttttgtc attccgagtc cttttttaat
     2221 gagaaaggaa tcttcaatct tattgattaa agatttctcc ttcagatcga aacgactaca
     2281 atgcttcttc attgcgaggc atcagaatta tcgtgagaaa atcacaattt attgtgtgag
     2341 cttgatccag cctatcgcct tcgcaatgac acttccatta ttttatcttt accgcgtgcc
     2401 cgccaaactg atttcttaat gctgctaata ctttcattgc aaatgattca tcctggcgcg
     2461 actgaaatct tgtatacagc gaagcagtaa taacgtgtgc cggaacgtca aaatccatcg
     2521 cagtttgcac agtccatctt ccctcaccac tatcctggac ataatctttt agcttttcta
     2581 atttcgggtc gtctttaaaa gcgttaacag ctaactcaag aagccacgaa cgaacaacgc
     2641 ttccatattg ccaggcatct gcaactttag tgatatcgat tttataaggt gatttttcta
     2701 atatctcaaa accttcagca taagcctgca tcattccata ttcaattccg ttatgaacca
     2761 ttttaacaag atgacctgta ccggattgac cacaataaac gtaaccgttt tcaggtgcaa
     2821 gtgatttaaa gattggttca acaaagtcag tcgcattttt ttcaccgcca tacattaaac
     2881 aatatccgtt ttgtaacccc cacacgccgc cgctagtacc gcagtctaag taatgaattc
     2941 gatacttagc taatttttct gaacgctttt gtgtgtcttt ccaatacgaa tttccaccat
     3001 caataattat atcgtcttct tttaatagtt tttgaagaga gtcaatattt tcatcaac
//
LOCUS       DASWBY010000136         3502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_380791, whole genome shotgun sequence.
ACCESSION   DASWBY010000136 DASWBY010000000
VERSION     DASWBY010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380791"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VF870_02575"
     CDS             <1..1135
                     /locus_tag="VF870_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX9251093.1"
                     /translation="PSFQYYPDYFKIIKLSGGSATVSLKDYDGDGDNDLLSGDWLGKF
                     NYFRNDGTPEKPIFNKVTTSFSNLTVNSYSSPVFIDIDKDGDYDIVSGALSGQVFLFV
                     NNNGTFSQNTTMFSSIDVGWTSIPSFADLDADGDLDLLVGGETGSESKYYTNDGSNVF
                     TENTTTFSGVTFPNYGRPTLADIDNDGDYDLIIGDGWGKVFYYRNDGNVSTPVWVRAD
                     EMFAGIEVDQNAHPGFADLDNDGRRDMIIGEYNGNFTYYKNLFSPLTSIEEENILAAP
                     TEFVLYQNYPNPFNPTTKIRFTVPNVTLNISSGAGSRGELSQLQLKVYDILGKEVATL
                     VNEEKPAGTYEVDFDASGLSSGVYIYTLKVNGVSHSKSMILLR"
     gene            complement(1144..1605)
                     /locus_tag="VF870_02580"
     CDS             complement(1144..1605)
                     /locus_tag="VF870_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251094.1"
                     /translation="MENQNKSVKYFFIYAIFIAMILLITGCDDCSDGNYKPYDCLERE
                     PAEGILRIEVTLDGLNSVVPIEVYYGDYENNAFYFADTLDSFDYEYWVPNGDYSVKAK
                     YSAMINNRLVTVYSIDGGNISSSVTNYCDGDCYSEGRLVLNAKLDLNSALQ"
     gene            complement(1605..2186)
                     /locus_tag="VF870_02585"
     CDS             complement(1605..2186)
                     /locus_tag="VF870_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251095.1"
                     /translation="MIQIKTSKNIKRLEIILFSFLLIFPTENFYAQDDGTSKPKFLIE
                     GGTAFNEDFFYLGGDMSFLILAKQDVYFVLSGYFNPTARKVLHQESSDVYLLLSEYRV
                     WSGLGLQKNLILNRTISLFVRAEVGWGWINYAGSNKDFNDPVAPVFTGGFQFSIPLKD
                     GGPSQILIKTAYQYLRFGSLDSHLALITFGVGF"
     gene            complement(2371..>3502)
                     /locus_tag="VF870_02590"
     CDS             complement(2371..>3502)
                     /locus_tag="VF870_02590"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9251096.1"
                     /translation="STFPEEVEPYFDSICIGEAHKVWAEMVNDLLSGKLKKRYDAGLA
                     PDLENIPAPRYDLVESNYFTPIVTEASRGCPHPCTYCQLNIKRAPYRTRPVEDVIRDL
                     TNTKGLPFIKRKTAMILDNNLGGSLTNAKELLREIAKLKFWAIGAQYSIECLRDDEFV
                     ELMGKANCRMSFLGMESLNEESLRDVEKKQNKVEEYKEFFYKLNKKGILTFTGFMFAL
                     DEDTPEYYETLSQKLEEVGVNVILPSISIPIYGTPLYKKIVDENRLIDNDITHYEGDH
                     VLFKHKHLTEEQIYYYYEKIIKIFYSWKNILKRWWKFASMQTFDGSIKEYLLKTVLGS
                     VIYFKLSIFQRHHAQERVFNKRRNVKSLNSLIPKSELKVKAA"
BASE COUNT         1116 a          606 c          585 g         1195 t
ORIGIN      
        1 accatctttc caatactatc ctgattactt taaaatcata aaattaagcg gaggaagtgc
       61 aaccgtttct cttaaagatt atgatggaga tggtgataat gatttattaa gcggagattg
      121 gcttggaaaa tttaattact ttagaaatga tggcactcct gaaaagccaa tatttaataa
      181 ggttacaact tcgttctcaa acttgactgt aaactcttac agctctcctg tttttattga
      241 tattgataaa gacggagatt atgatattgt ttcaggtgcg ctaagcgggc aggtttttct
      301 tttcgtaaat aacaacggta cattctctca aaatactact atgtttagtt caatagatgt
      361 tggatggaca agcataccgt cttttgcaga tcttgatgca gatggagatt tggatttact
      421 ggtcggcgga gaaacaggaa gtgaatcaaa gtattataca aatgatggaa gcaatgtctt
      481 tacagaaaat actactactt tttcaggtgt aacatttcct aattacggaa gaccaacttt
      541 agcagatata gataatgatg gtgattatga tcttataatt ggagatggtt ggggaaaagt
      601 gttttattat agaaacgatg gaaatgtcag tacaccagtc tgggtgagag ctgatgaaat
      661 gtttgccgga atagaggtgg atcaaaatgc tcatccgggt tttgccgatc ttgataatga
      721 tggaagaaga gatatgatta tcggggagta caatggaaac tttacttact ataaaaattt
      781 attttctcca ttaacaagca ttgaagaaga aaatattttg gccgcaccga cagaatttgt
      841 gctgtatcag aattatccaa atccatttaa tccaactaca aagattagat tcacagttcc
      901 gaatgtcacg cttaatatat cctctggagc aggatcgaga ggagaattat ctcaacttca
      961 gctaaaagtt tatgatattc ttggtaaaga ggttgcaact ttggttaatg aagaaaaacc
     1021 cgccggtaca tatgaagtag attttgatgc atccggatta tcaagcggtg tttatatcta
     1081 cacattaaaa gtaaatggag tttctcactc taagagtatg atactattaa gataatgtta
     1141 aacttattgt aaggcagagt ttaaatcaag ctttgcattt aaaactaatc tgccttctga
     1201 gtagcaatcg ccgtcacaat aatttgttac tgaagaagaa atgtttcccc catcgatgga
     1261 ataaacagta accaatctgt tattaatcat tgcactgtat tttgctttaa ccgagtaatc
     1321 accgtttgga acccaatatt cataatcaaa tgaatcgagt gtgtcggcaa agtagaaagc
     1381 attgttttca taatctccat agtaaacttc aataggtaca acagagttta atccatccag
     1441 agtaacttca attctaagta tgccttctgc cggttctctt tctaaacaat cataaggttt
     1501 ataatttcca tcactgcaat catcgcagcc ggttattaat aatatcatag ctataaatat
     1561 cgcataaata aaaaagtact ttactgattt attttgattt tccattaaaa tcctactcca
     1621 aatgtaatta aagctaaatg tgaatctaat gaaccgaatc taagatattg atatgcagtt
     1681 ttaatcaaaa tctgagaagg gcctccgtcc ttaagcggga tcgaaaactg aaatccaccc
     1741 gtaaatactg gtgcaacagg gtcattaaaa tctttattgg aacccgcata attaatccaa
     1801 ccccatccaa cctccgctct tacaaacaaa cttattgttc tgttaagtat taaatttttc
     1861 tgtaatccta atccgctcca aactctgtat tctgatagca gcaaatagac atcgctggat
     1921 tcctgatgta aaacctttct tgcggtggga ttaaaatatc cgcttaatac aaaataaaca
     1981 tcctgtttgg ctaatataag aaaggacata tctccaccca gatagaaaaa atcttcatta
     2041 aaagctgttc cgccttctat taaaaatttt ggttttgatg tcccatcatc ctgagcatag
     2101 aaattctcag ttggaaatat cagtaaaaaa ctaaatagaa ttatttcaag gcgtttgata
     2161 ttcttacttg ttttaatttg tatcattttt ttgctgatgc tagatttttt aaggatttaa
     2221 gttttaatgc gcagcattta acaatccaaa tattgtgcct tttaaaaata ctctttcagt
     2281 tcaaactaag ttattatttt ttaaacagtg tttaattttc tttaaaaaca tgtccaaaat
     2341 taaaaacaaa taatcaatct agtgcaaaat ttatgcggct ttcactttta attctgattt
     2401 aggaattaaa ctgtttaagg attttacatt ccttctctta ttaaataccc tttcctgggc
     2461 gtgatgtctt tgaaagattg aaagcttaaa gtagataact gatcccaaca ctgttttaag
     2521 taagtattct tttatggatc cgtcaaacgt ctgcatcgaa gcaaatttcc accatctttt
     2581 caggatattt ttccaggagt agaatatttt tataattttt tcgtagtaat agtatatctg
     2641 ttcttctgta aggtgtttat gtttgaataa gacgtgatct ccttcataat gagtgatatc
     2701 gttatcaatc agtctgtttt catcaactat ctttttgtac aacggtgttc cataaatagg
     2761 aattgatata gatggcagaa ttacattaac tccaacttcc tctaacttct gagaaagtgt
     2821 ttcgtaatat tcaggagtgt cttcatcaag agcaaacata aatcccgtaa atgttaaaat
     2881 gcctttcttg tttagtttgt aaaaaaactc tttgtactct tcgactttgt tttgcttttt
     2941 ttcaacatca cgtaagctct cttcatttag agattccatc ccaaggaatg acattctgca
     3001 atttgctttt cccattaatt ctacgaactc atcatcccgc aaacattcaa tactgtattg
     3061 tgcaccaatt gcccagaact ttagtttagc tatttctctt aaaagttctt ttgcatttgt
     3121 taaactgccg ccgagattgt tatcgagaat catcgcggtt tttcttttaa taaacggtaa
     3181 tccttttgtg tttgtcaaat ctcttattac atcttcaacg ggtcgtgttc tgtaaggagc
     3241 gcgttttatg tttaactgac agtatgtgca aggatgcgga caacctcgtg atgcttcggt
     3301 tacaataggt gtaaaataat ttgattcaac aagatcatat cttggtgctg gtatgttttc
     3361 caaatcgggg gctaatccgg catcatatct ttttttcaat ttacctgata acaaatcatt
     3421 aaccatttca gcccaaactt tatgagcctc gccgatacaa attgaatcaa aatatggttc
     3481 aacttcttcc ggaaatgtgg aa
//
LOCUS       DASWBY010000137         6567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_418855, whole genome shotgun sequence.
ACCESSION   DASWBY010000137 DASWBY010000000
VERSION     DASWBY010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418855"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3472
                     /locus_tag="VF870_02595"
     CDS             <1..3472
                     /locus_tag="VF870_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251097.1"
                     /translation="GVIAQPGAEGPRGSWKYDANGYIGDVSPVVGLRLPIRDYRRGSN
                     PLDGINDTIYSVIITPVDRPGGGEIGYDGALQSTFEPIPGFAFSGDLGVGKGVAMSHL
                     PETWPAQWPDYPDWTYSGDPIIVDGIDKTPLVDWNGFFGRGQLNADQESYFWMDDNND
                     EENYFIQGFLPDANDLSRRGHALQVSVRGFQWSNFLAQDVLFWLYNIKNDGTENYDQA
                     TFGTLVGTYVGVEAPEYNDDASFFNVRESITYTWDFDHYISPSANPKWLPDPTQVGYV
                     AYAFLESPGNGFDGIDNDGDDKDLGTSPFFVENDFKNLANQTPVAAGDKLVLIDKNTF
                     ERSLFTMPNDTVTVTSMGVKFFLKPGVTKLVEGDINAVTTDPNKNSRDGIDNDLDGLI
                     DENYQVHYRQYKKTTAVPPAQPTVLIDTLAPVQYVDFRNGYGITIPKDKMLDERRDDL
                     IDNDGDWNPEYDDLGADGKPGTGDFGEGDGIPTPGEPNFDATDIDESDQLGLTSFQYF
                     VPAGSITMSDDANMWSRITPGFFDVPTSIQNNVATRGEDGDFIYGSGYFPLLAGRTER
                     FSLALCYGSDYNAVLKTKQIAQTIYNANYNFPRPPDKPTLTVVPEDGKVTLYWDRVAE
                     ESIDPTLRVKDFEGYKIYKGTDPDLTDALSITNVYGEKVFYKPIAQFDLIDGKKGIFP
                     SSSTLLELVGGVSFNLGTDNGVQNFYVDTDVLNGRTYYYALVAYDFGDPTKDIYPSEN
                     TRFISKDALGRVSTDINTAYVIPNAPVAGYVPPPSGQSVDRVTGYSTVTPYSVVLDPV
                     RVLNASYNIEFNDSIFIKPLQREGVNISTNYTVREAGTGTILVDQAPFDPDNGVVFNG
                     VWLSIDSSYQRLDSIKLKPVGLNKEEGSFANDSSGWSTYRTKNLKFLVDQINTTSISG
                     TRYPLDYMLVFSDSYTDSSNKLTAIFGNNAPPAKAVNFRAYDITDKSAPKRVQFAFSE
                     PRPFRKDTLSFGDIVVFSDQTGNDLSWRLQFTGTDSSATIPAGGDTLFLYFYKPISGG
                     DSFTFSSSKPTYDLDGAREQLNLVRAVPNPYVVTNVFEQPLPPTVRGRGERIINFINL
                     PPKSTISIYTSSGNLVQTLQHDGDLNNGSVSWDVRTKEGLDVAFGVYFYVVEVEGLSD
                     KKMGKLAIIK"
     gene            3504..4508
                     /locus_tag="VF870_02600"
     CDS             3504..4508
                     /locus_tag="VF870_02600"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9251098.1"
                     /translation="MKKILLIILAFVFAGTVFPQTKVGTTVANFLTIPVGPRATGMGG
                     AFTAVANDATTSFWNPAGLSRIPRNEFTATTAEWLVSSRTNWVGLAVKFDDDNAIGIS
                     VNQLDYGQEEITTVQQQNGTGAYWSASDLAVSLSYSRNLTDRFSIGGTVKYISENIYD
                     VSATGFGVDVGLLFYTQLQGLSIGMNISNFGTEMQLDGKSLLQPIDVDPANAGNNSNI
                     SGQLLTESWPLPLTFAVGLAYDAINNEDWRLTLAADALIPNNQTTYGNAGLEITYANL
                     ISLRGGYNAIAKEDAQEGLTAGVGVQYDFGGFFAKFDYSYTDFGIFDPISRFALSVGL
                     "
     gene            complement(4575..6509)
                     /locus_tag="VF870_02605"
     CDS             complement(4575..6509)
                     /locus_tag="VF870_02605"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9251099.1"
                     /translation="MSKKEIKQKTVINKEKQTEKKSKKSPIWFYVVMILIPVVFIVGL
                     EVLLRFLNYGYDFQQFITTSSYHKDKIFLNPDLPRKYFFNIKSSPSVLPDGFDIQKKD
                     NAFRVFVLGESSAAGWPYVPNASFPRHIKRKLELLYPQNTIEVINCSMSAINTYTLRD
                     FVPGILEQKPDLVLIYTGHNEYYGALGVGSSVSLGYSRALVNTYIKLIDFKTTQLLQN
                     IVSSIYGLFRSSDEKGGEEGNETLMSRMIGESLISYKSDLYNYGIEQFDGNMDDILKM
                     LKEKNIPVILGTLTCNLKDQKPFVSVKDDNYPPAEDVFYQAKDFYQKGDIKKAKELFF
                     QAKELDALRFRAPQKINESINRLSKKYGYPVIDIDSAFESHSTNGIVGENLMVDHLHP
                     NLKGYKIIGEEYFKAMTNYKLLPKGTRAPLSETKVDSILTANFPFTRLDSTIAEMQLM
                     ILTGQYPFTPKGTVNYKVLNYKITDIVDSIAVQVINKDLRWETAHATLSNYYFLKKDY
                     ARCIHEMEAVIAERPYYDVPYKDLITKLVDGEKLDDAEIFLEKLYSLKADYFACKWLG
                     QVKLKKNESKDALKYLLEAVKFKEADSQTWYNLAGAYYLNKQEQKALEALQNSISLNP
                     QNRLAINFYNQLRKVLENKK"
BASE COUNT         2015 a         1136 c         1277 g         2139 t
ORIGIN      
        1 cggagttata gctcagccag gagcggaggg tccaagaggg tcctggaaat atgatgctaa
       61 cggatatatc ggtgatgtat ctccagttgt aggtttaaga ttacctatcc gtgactatag
      121 acgtggatca aatccgcttg acggaataaa tgatacgatc tacagtgtta ttatcacccc
      181 cgttgataga cccggaggag gtgaaattgg atacgatggt gcattacaat ccacatttga
      241 acctattccc ggatttgctt tttcaggcga cttaggtgta ggcaaaggtg tagcgatgag
      301 tcatttaccg gaaacctggc ctgctcaatg gcctgattat cctgattgga catactcagg
      361 agatccaata atagtggatg gaattgataa aactccatta gttgattgga atggtttttt
      421 tggccggggt caacttaatg cagatcagga aagctacttt tggatggatg ataataatga
      481 tgaagaaaat tattttattc aaggtttcct tccagatgct aacgatctat caagaagagg
      541 ccatgcattg caggtttctg taagaggatt tcagtggtca aatttcttag cacaggatgt
      601 attattctgg ctttacaata ttaagaatga cggtacagaa aattatgatc aggcaacgtt
      661 cggaacactt gtaggtactt atgttggtgt tgaagcacca gagtataatg acgatgcttc
      721 attttttaat gttagagaat caataactta tacatgggat tttgatcact acatttcacc
      781 ttcggcaaac cctaaatggc tgccggatcc aactcaggtt ggttatgtag cgtatgcatt
      841 cttagaatcg ccaggcaatg gatttgatgg aattgataat gatggcgatg ataaggattt
      901 aggcacatca ccattttttg tggaaaatga ttttaagaat ttagctaatc aaactcctgt
      961 agctgctggt gataaattag tgcttattga taaaaatact tttgagagaa gcctttttac
     1021 aatgccaaat gatacggtta ctgtaacttc catgggtgtt aaatttttct taaaacccgg
     1081 tgtaacaaaa ttagttgaag gtgacattaa tgcagtaaca acagatccaa ataagaattc
     1141 cagagatgga attgataacg atcttgacgg tctgatagat gaaaattatc aggttcatta
     1201 cagacaatac aaaaagacaa cagctgttcc acctgctcag ccaacagttc tgatcgatac
     1261 tttagctcct gttcagtatg tagattttag gaatggatat ggaatcacaa ttcctaaaga
     1321 caaaatgctg gacgaaagaa gagatgattt aattgacaat gatggtgact ggaaccccga
     1381 atatgatgat cttggtgcag atggtaaacc aggaacaggt gattttggcg aaggcgatgg
     1441 aattccaaca cctggagaac caaattttga tgcaactgat atagatgaat cagatcagct
     1501 tggattaaca agctttcaat actttgttcc tgcgggcagc atcacaatga gtgatgatgc
     1561 aaatatgtgg agtagaatta cacctggttt ttttgatgtt ccaacatcaa tccagaataa
     1621 tgttgctaca agaggtgaag atggagactt tatttatggc tccggatatt ttccactgct
     1681 ggcaggaaga acagaaagat tttctttagc actctgttat ggaagcgatt ataatgctgt
     1741 tcttaaaacc aagcaaattg cacaaacaat ttataatgca aattataact tccccagacc
     1801 tccggataaa ccaacactta ctgttgtacc agaagatggt aaagttacac tatattggga
     1861 tagagttgca gaggaatcaa ttgatcctac attacgagta aaggattttg agggatataa
     1921 aatttataaa ggtacagatc ctgatttaac tgatgctcta tcaattacaa atgtttacgg
     1981 cgaaaaagta ttttacaaac ctattgctca atttgatctg atcgacggga agaaagggat
     2041 ctttccttcc tcaagtacac ttcttgagct agtgggcgga gtttctttta atcttggtac
     2101 agataatggg gttcagaatt tttatgttga tacagatgtt ctgaatggaa gaacatatta
     2161 ttatgcactt gttgcgtatg attttggtga tccaacaaaa gatatttatc cctctgagaa
     2221 tacgcgcttt atttctaaag acgctcttgg cagagtttca acagatataa acactgcata
     2281 tgtaattccc aatgcacctg ttgcaggtta tgttccacct ccatcaggac agagtgtaga
     2341 cagagttacc ggatattcaa cggtaacacc ttattctgtg gtgcttgatc cggttagagt
     2401 tcttaatgca agttataata ttgagtttaa tgactcgata tttataaaac cgcttcagcg
     2461 cgaaggtgtg aatatttcta caaactatac agtaagagaa gcaggtactg gcaccattct
     2521 ggttgatcag gcgccatttg atcctgataa cggcgtggta tttaatggtg tctggctttc
     2581 aattgatagc agttatcaaa gattagacag cattaaactt aaacctgttg ggcttaacaa
     2641 agaagaaggc agttttgcta atgattcctc cggatggagt acttatcgga ctaaaaatct
     2701 aaagttttta gttgatcaga taaacacaac atctatttct ggcaccagat atccgctgga
     2761 ttatatgctt gtattttctg attcttatac agacagttcc aataaattaa ctgcaatatt
     2821 tggtaataat gctccacctg ccaaggcagt taattttaga gcttatgata taacagataa
     2881 aagcgccccc aagagagtac agtttgcatt cagcgaacca cgacctttcc gtaaggatac
     2941 actttctttt ggagatatag ttgttttttc agatcaaacc gggaatgatc tttcctggag
     3001 attacaattt accggaacag atagttcggc cacgataccc gctggcggcg atactttatt
     3061 cttatacttc tacaaaccaa tttccggtgg ggattcattt acctttagct catccaaacc
     3121 aacatatgat cttgatggag ccagagaaca actgaatctt gtaagagctg ttcctaaccc
     3181 atatgttgtt acaaatgttt ttgagcagcc tttgcctcca actgtaagag gaaggggtga
     3241 aaggattatt aactttatta atcttccgcc caagagcaca atttcaattt atacttcaag
     3301 cggaaattta gttcaaactt tgcagcacga tggagattta aacaatggct cagtttcctg
     3361 ggatgtcaga acaaaagaag gtctggatgt tgcctttggt gtttacttct acgtagttga
     3421 ggtagagggt ctaagcgata aaaaaatggg caaactggca ataattaaat aagcgattac
     3481 cagtgaatat tatggagatt aaaatgaaaa aaatattgtt aataatacta gcttttgttt
     3541 ttgctggaac agtttttcca caaacaaaag taggaacaac agttgcaaat ttcttaacca
     3601 ttcctgttgg tccgcgtgct accggaatgg gcggcgcatt tactgcggtt gctaacgatg
     3661 caactacatc tttttggaac ccggcaggat tatctcgaat tcctagaaat gagtttactg
     3721 caaccactgc tgaatggctg gtaagttcca gaaccaactg ggttgggttg gctgtaaaat
     3781 ttgatgatga taatgcaata ggtataagcg tcaatcaatt ggattatggt caagaagaaa
     3841 ttacaacggt tcagcagcaa aatggaacag gagcttactg gtcggcatcc gatttggctg
     3901 taagtctttc atattccaga aatcttacag atagattttc tattggcggc acagttaaat
     3961 atatctcaga aaatatttat gatgtttctg ctactggttt tggcgtagat gttggattac
     4021 tcttttacac gcagcttcag ggcttaagta tcggtatgaa tatttccaac tttgggactg
     4081 agatgcagct tgatggaaaa tcactattac agcctataga cgttgatcca gctaatgcag
     4141 ggaataactc taatatatca ggacaattac ttacagagtc ttggccactg cctctgactt
     4201 ttgctgttgg tctagcttat gatgctatca ataatgagga ttggcgtttg acattagctg
     4261 ctgatgcatt gatacccaat aatcaaacta catatggcaa tgcaggattg gaaattactt
     4321 atgctaattt aatttcattg cgtggcggtt ataatgcaat cgcaaaggaa gatgctcagg
     4381 aaggattaac agccggtgta ggtgttcaat atgatttcgg cggtttcttt gcgaagtttg
     4441 attacagtta cacagatttt ggtatttttg atcctatttc aagatttgca ttatctgtag
     4501 gactttaaaa tagaataaaa ttttactttt taagtgttag cactttaaca agccccgcat
     4561 tacagcgggg ttttttattt tttattttca agcacctttc taagctgatt ataaaaatta
     4621 atagctaatc tgttctgagg atttaaactt atactattct gtaaagcctc aagagctttt
     4681 tgttcttgct tatttaaata gtaagctcct gcaagattat accaggtttg tgaatctgcc
     4741 tctttaaatt taactgcctc caaaagatat tttagtgcat cttttgattc attcttttta
     4801 agtttcactt gtcccagcca tttacatgca aaataatcag ctttaaggga atataatttt
     4861 tcaaggaata tttcagcatc atccagcttc tcaccatcta caagttttgt gataagatct
     4921 ttatacggaa catcgtaata tgggcgttcc gcaattacag cttccatttc atgaatacat
     4981 cttgcataat cttttttaag gaaataataa tttgaaagtg ttgcgtgggc tgtttcccat
     5041 ctaaggtcct tattaataac ctgcactgct attgaatcaa caatatctgt tattttgtaa
     5101 ttcaaaactt tataattaac tgttccttta ggcgtaaatg gatattgtcc tgttaaaatc
     5161 atcagctgca tttcagctat agtcgaatcc aatcttgtaa atggaaaatt tgcagtcagg
     5221 atgctatcaa ctttggtttc acttaatggt gcgcgtgtgc ctttaggaag caatttataa
     5281 ttagtcattg ctttaaaata ctcttcacca attattttat agcctttaag attaggatga
     5341 agatgatcaa ccattaaatt ttcaccgaca attccgtttg tgctatgaga ttcgaacgca
     5401 gaatctatgt ctataacagg atatccatat ttttttgata agcgattaat cgactcattt
     5461 attttttgag gagctctgaa ccttaatgca tccagctctt ttgcctggaa aaacaactct
     5521 ttagcttttt taatatcgcc tttttgataa aaatctttag cctgataaaa aacgtcttct
     5581 gctggcgggt aattatcatc ttttacagag acaaatggtt tttgatcttt taaattgcag
     5641 gttaatgttc caagaataac gggaatattt ttttctttga gcattttaag aatgtcatcc
     5701 atgttcccat caaattgctc tatgccgtaa ttatataaat cagatttata agagattaat
     5761 gattcaccga tcattctgct cattaaagtt tcgttgcctt cctctccgcc tttttcatca
     5821 gaagaacgaa acaatccata aatacttgat acaatatttt gtaaaagttg agttgtttta
     5881 aaatcaataa gttttatata agtatttact aatgctcttg agtaacccag ggaaactgaa
     5941 gaaccaaccc ctaatgcacc gtaatactcg ttatgccccg tataaataag tacaagatca
     6001 ggtttttgtt ccagtattcc gggaacaaaa tctctgagtg tgtaagtgtt tattgctgac
     6061 attgagcaat ttataacctc gattgtgttc tgcggataaa ggagttcaag ttttcttttt
     6121 atgtgtcttg gaaatgaagc attgggcaca tacggccagc cggcagcaga actttcacct
     6181 aaaacaaaaa ctctaaacgc attatccttt ttctgtatgt caaaaccatc cggtaaaaca
     6241 gagggagaag actttatatt gaagaaatat ttccttggca ggtcggggtt tagaaaaatt
     6301 ttatctttgt gataactgga tgtagtaata aattgctgaa aatcataacc atagttcaaa
     6361 aatctaagta aaacttccaa tccgactata aaaaccactg gtattaaaat cataactaca
     6421 taaaaccaga taggagattt tttactcttc ttctcggttt gcttctcttt gttaataact
     6481 gttttttgct ttatttcttt cttgctcata taatctataa ctctacaagt tcttttttgg
     6541 taaatgttaa taactttttt ttaagat
//
LOCUS       DASWBY010000138         3887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_606609, whole genome shotgun sequence.
ACCESSION   DASWBY010000138 DASWBY010000000
VERSION     DASWBY010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606609"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VF870_02610"
     CDS             <1..400
                     /locus_tag="VF870_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251100.1"
                     /translation="SKFFLKGTHFSMDFLNNTNYIRLTNFMEIVIEHSGTIKWHFAKC
                     NHTKCNSIFLVHPEENPGDLGFICPECSRKIHTSHIVQCTSCRTVLNFVRAAPNEEKV
                     VFTVPKCSHCIGTIEDEWEIEPLYQPDSYI"
     gene            568..2724
                     /locus_tag="VF870_02615"
     CDS             568..2724
                     /locus_tag="VF870_02615"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX9251101.1"
                     /translation="MRFRFGALAKQITLSVILLSFFFSSQIFSQSSNWMNLDTVKAGK
                     FDTGKMWTFEYPPMDYFSQAYNFKPDEAWFEHVRMSALRFANYCSASFVSADGLVMTN
                     HHCARQSITQLTKEGEDLNATGFIAQTLQDERPVPGLYVDQLVLFKDVTDEVIDEINK
                     GTTDEERAKFEEDIIAKIEEREAQATGLEVSITPLYNGGKFSLYGYKRYTDVRLVLAP
                     EDQMGFFGGDPDNFTYPRFNLDCSFFRVYDENGNPLKTENFFKWSENGAAVGEPVFVV
                     GNPGNTDRLNTVAQLEYARDVQYPRIVALLKNLSDMYKEMLKKDPSRKLELQDMIFSF
                     DNSVKAYGGILEGLRDPVLMQRKKDFEKTFRASVDADPEMKAKYGDLWDKIADTRKQL
                     SEISNKNFALSMNRLSTPQYFFIADEVIAIAENLKLPESERNDLYVGEELDNTLASLF
                     PEDFDAEMNNSLLEQKIDALYMYLGANDPLVKKFTGGKQGKEAVEYVLSHSKITNLED
                     VKDLLKEGPDEVLKGDDPFIYFMMNSKDQAKEYSQKIKQITDEESSYSQQLGMALFKV
                     YGTSIPPDATFTLRLADGVVEGYPYNGTYAQPFTTFYGMYDRYYGQGKEFPWSLPERW
                     TPPPADFDLSTPFNFVSTNDIIGGNSGSPVINKNAEIVGLAFDGNIQSLPGSFIFRTE
                     ENRTVSVHSKGMWEAIDKVYKLKRLSAELKAGKITN"
     gene            2951..3379
                     /locus_tag="VF870_02620"
     CDS             2951..3379
                     /locus_tag="VF870_02620"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9251102.1"
                     /translation="MNTIKRQTIAVIALLLLFVMAVNMNVFAQPNPISNVTKNEYALD
                     NLKAAINSENTGVRKSSIYFAGKYKIAEAVKCLVERLENEEDASVRLLIAYSLYEIKD
                     EAGMKAVKQLSLNDDDIKVKKISFNLYEKYLNNGGSTAAL"
     gene            complement(3444..>3887)
                     /locus_tag="VF870_02625"
     CDS             complement(3444..>3887)
                     /locus_tag="VF870_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251103.1"
                     /translation="GSFSGNSVSVGGFTTSIFVEANLPLFEEVFPRVSLIYTKDFNAI
                     LPNTNQPYNPFIMGINFKGVTTQYFDSKIFLEEGVGLTALNDRTFSDTDIWDYGIVVS
                     LSVGYDLRNFDLKGFKIGAGAEYGITFFNTLPKYSSIHVIVQFVI"
BASE COUNT         1328 a          619 c          708 g         1232 t
ORIGIN      
        1 atcaaaattt tttttgaaag gtactcattt ttcaatggac tttttgaata acactaatta
       61 cattcgctta acaaatttta tggagatagt tatcgaacat tctggtacta ttaagtggca
      121 cttcgctaaa tgtaatcata caaaatgcaa ctcaatcttt ttagttcatc ctgaagaaaa
      181 tcctggtgat cttggtttta tttgccctga atgcagcaga aagatacata cctcacatat
      241 cgtacaatgc acaagctgca gaacggtatt aaattttgta cgtgctgcac ccaatgaaga
      301 aaaggttgtt tttacggttc ctaaatgttc acactgcatc ggtactattg aagatgagtg
      361 ggaaattgaa ccattatatc agcctgattc atacatttaa tagcttttgt gccgaaccaa
      421 ttgattttta atctattttt aagtcaattg tgaattttat ccttttattc gaccattcat
      481 aatctgtatg gcgcatagaa ggatttttta tttatttttg ttttcgtctt ttaatcaata
      541 atatttaata cataataagg tgctgctatg agatttcggt tcggagcttt agccaaacaa
      601 ataaccctga gtgttattct tctcagtttt ttcttttcat cccaaatatt ttctcaatct
      661 tctaactgga tgaatcttga tactgttaaa gccggaaaat ttgataccgg taaaatgtgg
      721 acttttgaat atcctccgat ggattacttc agccaagctt acaattttaa accggatgaa
      781 gcctggtttg aacatgtaag aatgtctgca ctccgttttg caaattattg ttctgcttct
      841 tttgtttcag ccgatggttt agttatgact aaccatcatt gcgcaagaca aagcattacc
      901 cagcttacaa aggagggtga agatttaaat gccacaggat ttatcgcaca aaccctgcag
      961 gatgaaagac ctgtaccagg attgtatgta gatcagttag ttttatttaa agatgtaact
     1021 gatgaggtta ttgatgaaat aaataaaggc acaacagatg aagaacgcgc aaaatttgaa
     1081 gaggatatta ttgcaaagat tgaagaaagg gaagcacaag caaccggcct tgaagtatca
     1141 ataactcctc tttataacgg cggtaaattt tctctttacg gatataagag atatacagat
     1201 gtaagattag ttttagcgcc tgaggatcaa atggggtttt ttggcggcga tcctgataat
     1261 tttacttatc cacgatttaa cctcgattgc agtttcttta gagtttacga tgaaaatgga
     1321 aatccgttaa agactgaaaa tttctttaaa tggagcgaaa acggtgctgc agttggtgaa
     1381 cctgtttttg ttgttggaaa tccaggcaac acagatagat taaatactgt tgcgcaatta
     1441 gaatatgcca gagatgtaca atatccaaga attgttgcat tgctaaaaaa cttatcagat
     1501 atgtacaaag aaatgcttaa gaaggacccg agcagaaaac tcgaactaca ggatatgatt
     1561 ttcagttttg ataattcagt aaaagcttat ggtggaattt tagaaggatt aagagatcct
     1621 gttttgatgc aaagaaaaaa agattttgaa aaaacttttc gtgcatcagt tgatgctgat
     1681 cctgaaatga aagcaaagta tggtgattta tgggataaaa ttgctgatac ccgtaagcag
     1741 ctaagtgaaa tatcaaataa gaattttgct ttgagcatga acagattatc aacaccacaa
     1801 tacttcttta ttgctgatga agtaattgcg attgcagaaa atcttaaatt gccggaaagt
     1861 gaaagaaatg atctttatgt tggtgaagag ctcgataaca ctttagcttc actttttccg
     1921 gaagattttg atgctgaaat gaacaattca ttgcttgaac agaaaatcga tgcattgtat
     1981 atgtatcttg gtgcaaacga ccctctggta aagaaattta caggaggcaa gcaaggcaaa
     2041 gaggctgttg agtatgtttt atcccattca aaaataacaa accttgaaga tgttaaagat
     2101 cttttaaaag aaggacctga tgaagttctt aaaggtgatg atccatttat ttattttatg
     2161 atgaattcta aagatcaggc aaaagaatat tcccaaaaga ttaagcaaat tacagatgaa
     2221 gaatcatcat attcccagca attaggtatg gcgctattta aagtttacgg aacatctatt
     2281 ccgccagacg caacatttac attaagatta gctgatggtg ttgttgaagg ttatccatat
     2341 aacggaactt acgcacagcc ctttacaacg ttctacggaa tgtatgatcg ttattacggt
     2401 cagggaaaag agttcccctg gagtttgccc gaaagatgga caccaccacc tgcagatttt
     2461 gatttatcta caccgtttaa ctttgtttca accaatgata taattggcgg aaactccgga
     2521 agtcctgtta taaacaaaaa tgcagagatt gttggattag catttgatgg caatattcaa
     2581 agtttacctg gaagttttat ctttaggact gaagaaaaca gaactgtttc tgtccattca
     2641 aaaggaatgt gggaagcaat agacaaggtt tacaaactca aaagactgag tgctgaatta
     2701 aaagctggta agattaccaa ttgaaaataa ctaaataatt agactttaat tcccttcaaa
     2761 ttctgaaggg aattttttta tctatccata atctaatcgc attactcctg ccattttttt
     2821 ctctaaaata atcatgacaa ttttttacat tcatttactt gcgagtgaca ggcgtgacaa
     2881 agtatcgttt tagatttgaa ccagagatta acgataacat ttttataaat tatttagtga
     2941 gaggttcaaa atgaatacaa taaaacgaca aacaatagca gtaatagctt tacttttatt
     3001 atttgtaatg gcagtaaata tgaatgtttt tgctcagccg aatccaattt caaatgtgac
     3061 aaaaaatgaa tatgctttag ataatctaaa agcagctatc aattcagaga ataccggtgt
     3121 aagaaaaagt tctatttatt ttgccggaaa atataaaatt gcagaagctg ttaagtgcct
     3181 tgtagaaaga ttagagaatg aagaggacgc atcagttaga ttattaattg cttactcgct
     3241 ttatgaaata aaagatgaag ctgggatgaa agcagttaaa cagttatctc taaatgacga
     3301 tgacattaaa gtgaaaaaaa taagttttaa tctttacgaa aaatatttga ataatggtgg
     3361 ttctaccgca gctttataaa attaaatgtg cctctctaat gaatcccgcg tatcctattt
     3421 agagcgcggg ttttcttttt ttatcatata acaaattgca caataacatg gattgatgaa
     3481 tattttggca aagtgttaaa aaaggttatt ccgtattccg ccccggcacc aattttaaat
     3541 cccttcaaat cgaaatttct aaggtcgtaa ccaacagaca gcgaaaccac gatgccataa
     3601 tcccaaatat ctgtatcgga aaaagttctg tcattcaaag cagtcaatcc aacgccttct
     3661 tctaaaaata ttttgctgtc aaaatactga gtagtaactc ctttaaagtt aatacccata
     3721 ataaaaggat tatagggctg atttgtattt ggcagaattg cgttaaaatc ttttgtgtaa
     3781 ataagactta ctcttggaaa gacttcttca aaaagaggaa gattagcttc aacaaatatg
     3841 gaagtagtaa atccaccaac agaaactgag ttaccactga atgatcc
//
LOCUS       DASWBY010000139         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_7008, whole genome shotgun sequence.
ACCESSION   DASWBY010000139 DASWBY010000000
VERSION     DASWBY010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7008"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..>2300)
                     /locus_tag="VF870_02630"
     CDS             complement(19..>2300)
                     /locus_tag="VF870_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9251104.1"
                     /translation="YPNKTTELDVSMEEAVIEIESVQVTGMKVQQQNDTRTSLIDLNP
                     RNAKILPGAAEDVLRTLQSLPGVLAPNDFSSQLIVRGSGPDQNLIIMDDVEIFNPYRL
                     YGVVSMFNPDAVSDVNLMSGGFPAKYGDRLSAVLDVTNREGDNSKYLKGNVNVSIVDA
                     NIVLEGKNPLGLKGSWLVNSRRTYYDLVIEPFVKSAGLVDDNTSFPNFYDFQGKLVFG
                     PFNGHKILLNGILSRDGVDVVSGKERNTPDSVGVYNITRNDIVSAAWHFAPSKNYLNK
                     VIVSWYKNNGTTDFNSEILDPSLNREDFENTIPDTLKPYLLGFKFNGDFDYRKVTIDD
                     KFTYLWNENIFEAGAGVDFMETTMNFKFDLDPALEAIFAANPQFRAALSDLKDVKKYN
                     RYRVYAQNNFKVSEKLFINPGLRFDYYDLLDKPYIAPRLSFSYAIDEITTLRGVWGIY
                     YQSPGYEKLRDQNVLFDLADVYTKPLNAEKAIHYVIGIERWITNEWSLRFESYYKDFI
                     DLIVQQKVQGTAFITEPIPGRDPRLASSWTNPVAVPGDSLTQIPVNNSFGEAYGFEFF
                     LSKRNVMNNSRLSGWISYALAYANRFEDGIKLPFRFDQTHTFNFVLNYDFNNWFNVGM
                     RWQYGSGFPYSQPVGIRPRVILADKNGDGIPETPVISTRTSFNDPNGEGEVVYDVDFG
                     DNKFNSRKPAYHRLDLRFNFLADFWNLDWVFYLDVVNVYNRKNVVNYDYYVTKDLTLG
                     RAQNNMFPILPTFGVSVKF"
BASE COUNT          757 a          449 c          359 g          735 t
ORIGIN      
        1 cattattttc agttaaatct aaaatttaac acttacacca aacgttggca ggattggaaa
       61 catattattc tgtgctctac ccagagttaa atctttagtt acgtagtaat catagtttac
      121 aacatttttt ctgttataaa catttacaac atccagataa aacacccaat caagattcca
      181 gaaatctgca agaaaattaa atcttaaatc taatctatgg taagcaggtt tacgggaatt
      241 aaatttgtta tctccaaaat ctacatcgta aacaacttcg ccttcaccat ttggatcgtt
      301 aaagcttgtt cgtgtagaaa taacaggagt ttccggaata ccatcaccat tcttgtctgc
      361 taagattact cttggtctaa ttccaaccgg ttgactgtaa ggaaatcccg aaccatattg
      421 ccagcgcata ccaacattaa accaattatt aaaatcataa tttaaaacaa aattaaaggt
      481 atgtgtctga tcaaaacgga aaggaagctt tattccatct tcaaatctgt ttgcataagc
      541 aagcgcgtaa gaaatccagc cgcttaatct gctgttgttc attacatttc ttttggatag
      601 aaaaaattca aatccgtagg cttcaccaaa agaattgtta acaggaattt gtgtaagcga
      661 atctcccgga actgcaacag gattagtcca gctgcttgcc aatcgtgggt ctcttcctgg
      721 aattggctcg gttataaaag ctgtaccttg aactttctgc tgaacgatga gatctataaa
      781 atctttatag taagattcaa agcgcagact ccattcattg gttatccatc tttcgattcc
      841 aattacataa tggattgctt tttctgcatt aagaggcttt gtgtaaacat cagcaagatc
      901 aaaaagaaca ttctgatcac gaagtttttc ataccccggt gattgataat aaattcccca
      961 aactccgcgc agtgttgtta tttcatctat cgcgtaggaa aaagaaagtc taggtgcaat
     1021 gtatggttta tctaaaagat cgtaataatc aaatcttaaa ccgggattga taaaaagttt
     1081 ttctgatact ttaaaattat tctgtgcata aactctgtaa cgattgtatt tttttacatc
     1141 ctttaaatca cttagtgcag ctctaaattg cggattggct gcaaagattg cttcaagtgc
     1201 gggatcaaga tcaaacttaa aattcattgt tgtttccata aaatcaactc cggcacctgc
     1261 ttcaaaaatg ttttcattcc acaagtaagt aaacttatca tctatggtaa ctttgcgata
     1321 atcaaaatca ccgttgaatt taaatcctaa cagataaggt ttcaaagtat ccggaatggt
     1381 gttttcaaaa tcttctctgt tcagtgatgg gtcaagtatt tctgaattaa aatcagttgt
     1441 gccgttattt ttataccagg atacgataac tttatttaaa taattttttg atggagcaaa
     1501 atgccatgct gccgatacaa tatcatttct tgtgatgttg taaacaccaa cactatcagg
     1561 agtgttgcgc tctttaccgc taacaacatc aacaccatca cgagataaaa ttccatttaa
     1621 caaaatttta tgtccattaa aaggaccaaa aactaactta ccctgaaaat cataaaaatt
     1681 gggaaaagaa gtattgtcat caaccaaacc tgcgctcttt acaaaaggct ctatcacaag
     1741 atcgtaataa gttcttcgtg aattaactaa ccagcttcct tttaaaccta atggattttt
     1801 tccttccaga acaatatttg catccacaat tgatacgttg acatttcctt taagatattt
     1861 tgagttatca ccttctcgat ttgtaacatc taaaactgca gaaagtctat cgccgtattt
     1921 tgcaggaaat ccccctgaca ttaaattaac atctgaaact gcatcgggat taaacatact
     1981 tacaacaccg taaagtctat atggattaaa aatttcaaca tcatccataa taattaaatt
     2041 ctgatcagga ccgctgccgc gaacaataag ctgcgatgaa aaatcatttg gagctaaaac
     2101 accgggcaag gattgtagag ttcttaaaac gtcttctgct gcaccgggta atatttttgc
     2161 attacgcgga tttaaatcaa tcaaacttgt gcgggtatca ttttgctgct gtactttcat
     2221 tcccgttacc tgaactgatt caatttcaat tacagcctct tccatcgaga catccagttc
     2281 agtagtttta ttaggataga
//
LOCUS       DASWBY010000140         4276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_493947, whole genome shotgun sequence.
ACCESSION   DASWBY010000140 DASWBY010000000
VERSION     DASWBY010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493947"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VF870_02635"
     CDS             <1..1283
                     /locus_tag="VF870_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9251105.1"
                     /translation="PIKSKVQKFVDKKFFRIQYNFRKELNRFTAQIKNYNDVYSLSEY
                     LIKEINTLIPVDKIAFGELDTQTGKLFIRAHINFDQIANKILRIKPETLERKWFQVAA
                     VKNKVEGEANISTIFQNTLVRWKINLVVPIKSVNDELFGFIILGNKKSGSRFTVEDVD
                     LLKDIGINTGTTIERIKLQEQLILEKLAAEKLEELNQQKSMFVSTVSHDLKTPLTSIK
                     IFAEMLLENENSLSEKSKNHLEIIEGETDRLTRLINNVLDFSKIEKGVKDYSFREVHF
                     NKIVKSVIELMQYTLKMKGFNLATDLGDFNDLICGDADAITEAIENIISNAIRFSNDN
                     KEISIATYYKDNFACVDVKDYGIGIDSADFQKIYDPFFRSEDAKLKKIEGTGLGLPIV
                     KHIVDEHKGKILIESTKGIGSTFTLCFPVLTNNK"
     gene            1291..1971
                     /locus_tag="VF870_02640"
     CDS             1291..1971
                     /locus_tag="VF870_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9251106.1"
                     /translation="MKKVLIIEDDPAIRTGLKEALTTEGYSVSEADTGTKGFEIASKG
                     NFDLIVLDLILPGKDGIEICKDLRSDGVKTPIIMVTSRKEEIDKILGLEIGADDYVTK
                     PFSLRELLARVKALIRRSTYEPGDIEEVAFANLKIDFKKQEMFKGENSVKLSATEYRI
                     LHYFIDHEGEVVSRDKFLDEVWGYDTYPTTRTVDNYILSLRKKIEDDPADPKHLLTVY
                     KVGYKFQK"
     gene            2111..3544
                     /locus_tag="VF870_02645"
     CDS             2111..3544
                     /locus_tag="VF870_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEX9251107.1"
                     /translation="MSSRRNFIKTSSLAVAGLSLSNFSFTKSFFPNTFQSKRPPISAR
                     KFVSEAIEAKIIEMKKGIKDEELAWLFENCFPNTLDTTVNYKIIDGKPDTFVITGDIN
                     AMWLRDSSAQVWPYLQFVNGDKKLKDLIAGVINRQTKCILIDPYANAFNDGPGESQWK
                     NDITEMKPELHERKWEVDSLCYPIRLAFGYWKTTNDVSVFDENWKSAMKLVLKTFKEQ
                     QRKDGKSSYRFMRETAWATDSLPIGGYGNPINPVGLIVSSFRPSDDATIFSFLVPSNH
                     FAVVSLRQLAEIFNEVINDNVFAAECNSLADEVEEALNKYAIADHLNFGKIFPYEVDG
                     FGNKLFMDDANIPSLLSLPYLNAVEKADETYNNTRKFLFSRNNPYYFEGKAGNGIGGP
                     HAGLGKIWHLSVIMRGLTSTNENEIIDCIKLLKNSHANTGFMHESFDKDDAAKFSRPW
                     FAWANTLFGELLLMVQREYPHLLQKQF"
     gene            3552..>4276
                     /locus_tag="VF870_02650"
     CDS             3552..>4276
                     /locus_tag="VF870_02650"
                     /inference="COORDINATES: protein motif:HMM:NF037611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HEX9251108.1"
                     /translation="MNLFRKTFFLFLIIAQIIFSQNYVQYVDPFIGTGLNGHTYPGAI
                     VPFGMVQLSPDNGKSGWEYSSCYHYSDSLIVGFSHTHLSGTGIGDLYDIQFMPASFSK
                     NISDNEILKANFISKFDHQNESASAGYYSVVLKDYNITTELTAALRSGIQKHTFNNNG
                     NEFVQIDLGYSKNWDTPTETFIKVINEYAICGYRLSTGWAEDQRVYFYTEFSNPILKS
                     YLIQDSLFLKNLREVKGKTTRAI"
BASE COUNT         1532 a          648 c          777 g         1319 t
ORIGIN      
        1 agccaattaa atccaaagtt cagaaatttg tagacaagaa attttttaga attcaatata
       61 attttagaaa agagttaaac agatttaccg cacaaattaa aaactacaac gacgtttatt
      121 cccttagcga atatttaatt aaggaaataa atactttaat tcctgtagat aaaattgctt
      181 tcggtgagct tgatacacaa acaggaaaat tatttatccg cgcacacatt aattttgatc
      241 agattgcaaa taagatcttg cgaataaaac ccgaaacatt agaaagaaaa tggttccaag
      301 ttgccgctgt taaaaacaaa gttgaaggtg aagcaaatat ttcaactatt tttcaaaaca
      361 ctttagtacg atggaaaata aaccttgttg tgcctattaa atctgtaaac gatgaattat
      421 ttggatttat aattctgggg aataagaaat caggttcacg atttactgtt gaagatgtcg
      481 atcttctaaa agatattggt ataaataccg ggacaactat tgaaaggata aaactacagg
      541 aacagcttat tcttgaaaaa ttagctgcag aaaaattaga agaacttaac cagcaaaaat
      601 caatgtttgt ttcaactgtc tcccacgatc tgaaaacacc gttaacatca ataaaaatct
      661 ttgcagaaat gcttcttgaa aacgaaaata gtttatccga aaaatcgaaa aatcatcttg
      721 aaataattga aggcgaaaca gatagactaa cacgcttaat aaataacgtt cttgattttt
      781 ctaaaatcga aaagggtgtt aaggattatt cttttcgtga agttcatttc aataaaattg
      841 ttaagagcgt aattgagctg atgcagtaca cattgaaaat gaaagggttt aatctggcta
      901 ctgatttagg tgattttaat gatcttatct gcggtgatgc tgatgcaatt actgaggcaa
      961 ttgaaaacat tatttcaaat gcaatcagat tctcaaatga taataaagaa atcagtattg
     1021 caacttatta taaagacaat tttgcttgtg ttgatgttaa ggattatgga attgggatag
     1081 atagtgcgga ttttcaaaaa atctatgatc cgtttttcag atccgaagat gccaaactta
     1141 aaaaaataga agggaccggt ttgggacttc ctatcgttaa acatattgta gatgagcaca
     1201 aagggaaaat attaatcgaa agcactaaag gaattggaag cacattcaca ttatgcttcc
     1261 cggttttaac aaacaataaa tgaggttaca atgaagaaag ttttaattat tgaggatgat
     1321 ccggcaatta gaaccggttt aaaagaagca ttaacaactg aaggatattc tgtttctgaa
     1381 gcagataccg gaacaaaggg atttgaaatt gcaagtaaag gtaattttga tttaatagtt
     1441 ttagatttaa tcctgcccgg taaagatggg attgagattt gtaaagactt aagaagcgat
     1501 ggagtaaaaa ctccaattat tatggtaacg agcagaaaag aagaaatcga taaaatactt
     1561 ggacttgaaa tcggggctga tgattatgta acaaagccat tcagtttaag agaacttctt
     1621 gcccgtgtaa aagcattaat tagaagaagc acttatgagc caggcgatat tgaagaagtg
     1681 gcttttgcga atttgaaaat agattttaaa aaacaggaaa tgtttaaagg agaaaattct
     1741 gtaaaacttt ctgcaacgga atacagaatc cttcactatt ttatcgatca cgaaggcgag
     1801 gtagtttcgc gtgataaatt tcttgatgag gtttggggat atgatactta cccaacaaca
     1861 agaaccgtag ataattatat tctttcgctc agaaaaaaaa ttgaagacga tccggcagat
     1921 ccaaaacatc ttttaactgt ttataaggtc ggttataaat ttcagaagtg agattttaaa
     1981 agtctccggc ttagtcagag ataattattt ttaattcaat aaaatcggtc tgataggatt
     2041 tgaagtatga atggcatctg ccattttctt gttaatcact atttttactt atacaaaaaa
     2101 gaaagtttag atgtcctcca gaagaaattt cataaaaacc agcagccttg cagtcgcagg
     2161 tttgtctcta tcaaatttct cattcacaaa atcatttttt ccaaacactt ttcaatctaa
     2221 acgaccacct atatcagcac gaaagttcgt aagcgaagct attgaagcaa aaataattga
     2281 gatgaaaaag ggaattaaag atgaagaact tgcctggctt tttgaaaact gctttcccaa
     2341 tacactcgat acaactgtta attacaaaat cattgatgga aaacctgata catttgtaat
     2401 taccggagat atcaatgcaa tgtggctgcg cgattcatca gcacaggttt ggccttactt
     2461 acaatttgta aatggagaca aaaaattaaa agatttaatc gcaggagtta taaatcgcca
     2521 aacgaaatgt attctcatcg atccatatgc aaatgccttt aatgatggac ctggcgaaag
     2581 ccaatggaaa aatgatatta cggaaatgaa gccggaactt cacgaacgca aatgggaagt
     2641 tgattcgctt tgttatccaa taagacttgc attcggatat tggaaaacca cgaatgatgt
     2701 ttcagttttt gatgagaatt ggaaatctgc aatgaagctt gttcttaaaa cttttaaaga
     2761 acaacaacga aaagatggaa agagttcata cagatttatg cgggaaacag cctgggcaac
     2821 ggatagttta ccaattggcg gatacggaaa tccgataaac ccggttggac taattgtttc
     2881 atcattccgc ccgagcgatg atgcaacaat tttttcattt cttgtcccat ccaatcattt
     2941 tgcggttgtt tcattaagac agcttgcaga aatttttaat gaagttatta atgataatgt
     3001 ttttgccgct gaatgcaatt cgcttgcaga tgaagttgaa gaggcattaa ataaatatgc
     3061 gattgcagat catttaaact ttggaaaaat atttccttat gaagttgatg gttttggtaa
     3121 caaattattt atggatgatg caaacattcc aagtctttta tctcttccct atctaaatgc
     3181 tgttgaaaaa gctgatgaaa catataacaa caccagaaaa tttttattca gtagaaataa
     3241 cccatattat tttgaaggca aagcgggaaa tggaattggc ggtccacatg caggtcttgg
     3301 aaaaatctgg catctttctg ttattatgcg tggattgacc agcacaaacg aaaatgaaat
     3361 tatcgactgt attaaactct taaagaactc tcatgcaaat accggattta tgcacgaatc
     3421 gttcgataag gatgatgctg caaaattttc tcgtccctgg tttgcatggg caaacacttt
     3481 atttggtgaa ttgctattaa tggttcaaag agaatatcca catcttttac aaaaacaatt
     3541 ttaagatatt tatgaattta tttcgaaaaa cctttttttt atttctaata attgctcaaa
     3601 taattttctc acagaattac gttcaatacg ttgatccatt tattggaaca ggattaaatg
     3661 gtcacactta tcccggagct atagttcctt tcggcatggt gcagttaagt cctgataatg
     3721 gtaaaagcgg ctgggagtat tcctcgtgtt atcattactc ggatagcctg attgttggat
     3781 tttctcacac tcatttaagc ggaaccggaa ttggtgattt gtatgatatt caatttatgc
     3841 cggcaagttt ttcaaaaaat atttctgata atgaaatttt aaaagccaat tttatatcaa
     3901 agttcgatca tcaaaatgaa tctgcaagtg ccggatatta ttctgttgtg ttaaaagatt
     3961 ataacattac aactgagtta accgccgcac ttagatctgg aatacaaaag cacacattta
     4021 ataataatgg aaatgaattt gttcaaattg atttgggtta ttcaaaaaac tgggatacac
     4081 caacagaaac tttcattaaa gttataaatg agtatgcaat ctgcggttat agattatcta
     4141 caggttgggc tgaagatcag cgggtttatt tttatacaga gttttcaaat ccaattctaa
     4201 aatcttattt aattcaagat agtcttttcc tgaaaaattt aagagaagta aagggcaaaa
     4261 caacaagagc aatttt
//
LOCUS       DASWBY010000141         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_7327, whole genome shotgun sequence.
ACCESSION   DASWBY010000141 DASWBY010000000
VERSION     DASWBY010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7327"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VF870_02655"
     CDS             complement(<1..814)
                     /locus_tag="VF870_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251109.1"
                     /translation="MSTIDWIVMCGFTGFIVFYGAWKTRKTKDVDDYLRTGKSSSWWV
                     VALSIMATQASAITFLSTPGQAYVDGMRFVQFYLGLPIAMIILSITAVPIYSKLNVYT
                     AYEYLEKRFDLKNRVLGSVLFLSQRGLAAGFTIYAPALILSVILGWELNLTIILIGIL
                     VIIYTSIGGTNAVNKTHVLQITIITIGMFAAFFMIYIHLPKDISVIDAAYIAGRMGKL
                     NAIDFSFNLSDRYTFWSGIIGGTFLMLSYFGTDQSQVARYLAAKNITQSRMGL"
     gene            complement(811..948)
                     /locus_tag="VF870_02660"
     CDS             complement(811..948)
                     /locus_tag="VF870_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251110.1"
                     /translation="MEDNSKNMIEDKPPFLGSWNKIYLLVFGELIVLIILFYLFTKAF
                     S"
     gene            complement(949..3420)
                     /locus_tag="VF870_02665"
     CDS             complement(949..3420)
                     /locus_tag="VF870_02665"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX9251111.1"
                     /translation="MKLKSILLFSIILCSIIFPQPKPSLSSSEIKLALNKLDVLGSVL
                     YIAAHPDDENTAAISYLAKGKLLRTGYLSLTRGDGGQNLIGPEQSEQLGVIRTQELLE
                     ARKRDGGEQFFTRAIDFGYTKSPDETFEFWDKEKILSDVVWVIRKFRPDFIITRFPPT
                     GQGGHGHHTASAILALEAFNLANDKNAFPEQLKYLTPWQPKRIFWNAWLPAIQNEKID
                     LSKVPTLNLGEYNPLLGKSYTEISALSRSMHKSQGFGSSGIRINTLNYFLLQKGDSVN
                     SDMFEGIDLSWARVDGGHEIHSLIQRTINEFNDENPEASLDNLILIYEKISNLKNDYW
                     KEVKLKDITELIRSCTGIWVEAISENEFVSVGDTLKVKSMIVNRSDLDLSITNVGIRN
                     LSDSKIGITKLVNGEPNTSNFSFLIPENMPISQPYWLQNDHPVGMFNITDPNLTGLPE
                     ENPKLVCDFTVNYKNVPITFSVPGFYRITDPVDGEVYKPVVITPPVTVNIEKDIYFFS
                     SNSEKEIKISVQSFKNSATGKIILNSKEGWKVEPGEIVFQFDKINQKKDYYVKVTPSG
                     NSSTSELSAKIMIDNKIYTKSLTRIEYKHFLPQTIFYESKSKLELFNFEKDGVKKIGY
                     IVGSGDKIPDFLTDLGFDVTIMDDKHFSSNNLDQFDVIITGIRAYNTRDNLAAFQKAL
                     IKYVEHGGTLISQYNTTGDLVVEPGVLPLKLSRDRVTDEHSKVEILKSDHQIFNKPFK
                     ITEDDFNGWIQERGLYFPNEWDNNYTALLSMSDKDETPKTGSLLAAKYGKGTFVYTGL
                     SFFREIPAGVEGAIKLFINLLYSGK"
     gene            3616..>4218
                     /gene="kdsA"
                     /locus_tag="VF870_02670"
     CDS             3616..>4218
                     /gene="kdsA"
                     /locus_tag="VF870_02670"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX9251112.1"
                     /translation="MQITLNQIVIGDNNPFVLIAGPCVVENEKMILETAEAISKITSD
                     VGVPFIFKSSFKKANRTNLQSFTGLGDKESIAILRKVKEKLNLSVVTDIHTESDVEIL
                     GDAVDILQIPAFLSRQTDLLIAAGKSCKIINIKKGQFLAPEDMKHAADKVVSTGNNKI
                     MLTERGTTFGYHNLVVDMRSLVIMRELGYPVVMDATHAVQL"
BASE COUNT         1360 a          768 c          683 g         1407 t
ORIGIN      
        1 gcaatcccat tctgctttgt gtgatatttt ttgcggcaag atatcgcgcc acctgcgatt
       61 gatcagttcc aaagtaactt aacattaaaa atgtaccgcc tattattcct gaccagaatg
      121 tataacgatc actaagatta aatgaaaaat ctattgcatt tagctttccc atccttcccg
      181 cgatatatgc tgcatcaata accgaaatat cctttggcaa atgaatgtaa atcataaaaa
      241 atgctgcaaa cattccgata gtaattatgg ttatttgaag aacatgagtt ttgttgaccg
      301 catttgttcc accgattgaa gtgtaaataa ttaccagtat accaataaga ataatagtaa
      361 gatttaattc ccacccaaga attactgaaa gtatcaatgc aggagcataa atcgtaaaac
      421 cggctgcaag tccgcgctga ctcaaaaaca gaacgcttcc aagtacacga ttttttaaat
      481 caaatctttt ttctaaatat tcataagctg tgtaaacatt tagtttgctg taaatgggaa
      541 ctgcagttat tgaaagaatt atcattgcaa ttggcaaacc cagatagaat tgcacaaaac
      601 gcattccatc tacataagcc tggccgggag ttgaaagaaa tgtaattgca ctagcctgtg
      661 ttgccattat cgataatgca accacccacc aggaactgct tttacctgtt cgcaaataat
      721 catcaacatc ttttgttttg cgggttttcc aggctccgta aaaaacgatg aagcccgtaa
      781 atccacacat aactatccag tcaatcgtac tcaactgaat gcctttgtga agagataaaa
      841 aagtataatt agaacaatca gttcgccaaa taccaataaa tatattttat tccacgaacc
      901 aagaaatggc ggtttatctt caatcatatt ttttgaatta tcttccacct atttgcctga
      961 gtataaaaga ttgataaata attttattgc gccttcaaca cctgccggaa tttctctgaa
     1021 gaaagataaa cctgtataaa caaaagttcc tttaccatat tttgcagcca ataacgatcc
     1081 tgtttttgga gtttcatctt tatcactcat agacaaaagt gctgtgtaat tattatccca
     1141 ttcgtttgga aaatatagac ctcgttcctg tatccagccg ttaaaatcat cttctgtaat
     1201 tttaaatggt ttgttaaaaa tctgatgatc gcttttcaaa atttctactt tggagtgttc
     1261 gtccgtaaca cgatcgcgtg ataatttaag tggtaatact cctggttcaa caacaagatc
     1321 accggttgtg ttatattgag agattaatgt tccaccgtgc tctacatatt taataagtgc
     1381 tttttgaaat gccgcaagat tatctctagt gttataagcc cgaatacctg taatgattac
     1441 atcaaactga tctaaattat ttgaactgaa atgtttgtca tccataatcg ttacatcaaa
     1501 tccaagatct gttagaaaat caggaatctt atctccggaa ccaacaatat atccgatttt
     1561 ttttacgcca tctttttcaa aattaaataa ttcaagtttt gattttgatt cataaaatat
     1621 tgtttgaggc aggaaatgtt tgtattcaat tcgcgttaaa cttttagtat aaattttgtt
     1681 atctatcata atttttgctg ataactctga agtagagcta ttgcctgatg gagttacttt
     1741 tacgtaataa tcttttttct gatttatttt atcaaactga aaaacaattt cacccggttc
     1801 aactttccat ccttctttac tattcaaaat gattttgccg gttgcagaat ttttaaatga
     1861 ttgaaccgat attttaattt ctttttcgct gttgctgcta aaaaaataaa tatctttttc
     1921 aatgttaaca gtgacaggtg gagtaataac cacaggctta tatacttctc catcaacagg
     1981 atctgtaatt ctgtaaaaac ccggaactga aaaagttatt gggacatttt tataattgac
     2041 agtaaaatca catactaact tcgggttttc ttcaggcagg cctgttaaat ttggatcagt
     2101 tatattaaac attccaaccg gatgatcatt ttgtaaccaa tacggctgtg agattggcat
     2161 gttttccgga attagaaaac taaagttaga agtgttcggt tcgccattta caagtttggt
     2221 aatcccaatt ttcgaatcac tcagattcct aatcccaaca tttgtaattg atagatcaag
     2281 atcacttctg ttaactatca ttgatttcac ttttaatgtg tctccaactg aaacgaattc
     2341 attttcagaa atcgcttcaa cccatattcc agtacaggaa cgaatcagtt ctgtgatatc
     2401 tttaagttta acttctttcc agtaatcatt ttttaagttt gatatttttt cataaatcaa
     2461 aattaaatta tctaaacttg cttcaggatt ttcgtcatta aactcattaa tagtcctttg
     2521 gatcaaagaa tgtatttcgt gtccaccatc aacccgggcc cagcttaaat cgataccttc
     2581 aaacatatct gaatttactg agtcaccttt ttgcagtaaa aagtaattta acgtgttaat
     2641 ccttatgccc gatgatccaa aaccctgact tttgtgcata gatctactta aggctgaaat
     2701 ttctgtatat gattttccaa gaagtgggtt gtattcgccc agatttaaag ttggaacttt
     2761 tgatagatca attttttcat tttgtatagc gggaagccat gcattccaga aaatcctttt
     2821 tggctgccat ggtgttaaat atttaagctg ctcaggaaac gcattcttat catttgccag
     2881 attaaaagct tccaatgcta aaattgcaga agcagtgtga tggccatgtc cgccttgtcc
     2941 ggttggagga aatctggtaa taataaaatc aggacgaaat tttctaatca cccagactac
     3001 atcacttaaa attttttctt tatcccaaaa ctcgaaagtt tcatcgggtg actttgtgta
     3061 accaaaatct attgctctgg taaaaaactg ctcgcctcca tcacgttttc tagcttcaag
     3121 cagctcctgc gttcttataa ctcctagctg ttctgactgt tccggtccga ttaaattctg
     3181 tcctccatcg ccacgggtaa gtgaaaggta gcctgtccgt aataactttc cttttgccag
     3241 gtatgaaatt gccgcagtgt tttcatcatc cggatgtgcg gcaatgtaaa gaacgctccc
     3301 aagtacatca agtttattta atgctaattt tatttctgat gaacttagtg atggcttagg
     3361 ttgagggaaa ataattgagc ataatattat gctgaagagt aaaatcgatt taagtttcat
     3421 tattattttc tgatgttatt aatcggataa aaagttgttt cgattatagc acattgaagc
     3481 taaaaatcca agataaaatg tttgaatggt aaattgaggc ataagaatta tttaataata
     3541 aaatcattca caaattactt attcataaag acctatattt ttcatatttt cacaagtaat
     3601 taaagaaaaa atcctatgca aataacatta aatcaaatcg taatcggtga taacaatcca
     3661 tttgtactta ttgccgggcc ctgcgttgtt gaaaatgaaa aaatgattct tgaaactgcc
     3721 gaagcaatta gcaaaattac atcggatgtc ggagtgccgt tcattttcaa atcaagtttt
     3781 aaaaaagcaa acagaaccaa cttacaatct tttaccggtt taggagacaa agaatctatt
     3841 gcgattctga gaaaagtaaa agaaaaatta aatctatctg tggttacaga tatccacact
     3901 gaatccgatg tagaaatatt aggcgatgct gtggatattt tacagatacc tgctttctta
     3961 tcacgccaaa cggatttatt aattgcagcg ggaaaatctt gtaaaattat taatattaaa
     4021 aagggacaat tcctggctcc cgaagatatg aaacacgcag cagataaagt tgtatcaact
     4081 ggaaacaata aaattatgct tacagaacgg ggtacgactt ttggttatca taatctggtt
     4141 gttgatatgc gatcacttgt gataatgaga gaacttggtt atccggttgt tatggatgca
     4201 actcacgcag ttcaattg
//
LOCUS       DASWBY010000142         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_119774, whole genome shotgun sequence.
ACCESSION   DASWBY010000142 DASWBY010000000
VERSION     DASWBY010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119774"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VF870_02675"
     CDS             complement(<1..1056)
                     /locus_tag="VF870_02675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX9251113.1"
                     /translation="MLIPDKISTYLNKRSSNKWKIENDHQKYFQKIIIVPSIAESNNL
                     PELIKSLEQNDELELLNTLLLIVVNNSLSSSDEVKNDNQKTLAYLRKIKSKVNISSID
                     ACTDGKEMDDKNGGVGLARKIGMDLALTKFDYLSLNKKIMICTDADCIVDTNYLSKIS
                     QDFNRNNYEAAVVNFSHDISSNDEETKAIICYEIFLRYYVLGLNFAKSDYAFHTIGST
                     MLCTPEAYVKVEGMNKRKAAEDFYFLEKLAKIYPIGEIKSTFVHPSKRGSWRVPFGTG
                     RSVDRYLSNERNEYLLYDPNCFVVLKTWLEIFYDMSLSQPELVSGSFERIGSEQEIPN
                     QVRNDNMVNKNSLIKIPK"
     gene            complement(1156..2127)
                     /gene="miaA"
                     /locus_tag="VF870_02680"
     CDS             complement(1156..2127)
                     /gene="miaA"
                     /locus_tag="VF870_02680"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX9251114.1"
                     /translation="MQYDLITILGCTATGKTKLAVQLADCFDGEIISADSRQVYRGMN
                     IGTGKDLSEYSINGKQIPFHLIDIIDPSEEFNLYSFQKGFYKSFNEIKNRNKLPFLVG
                     GTGMYLSSILQDYNLNKADFETYNQNLSSFSDDDLRAMLKVLNPSLHNSTDLNDRERI
                     VKAIAVSKSNSYIDLDQSREKSVNKKEKDFSVVSLLRNDSNKKFNSLNIGIYLSRDEI
                     KKRITARLKKRLNEERMIEEVKSLMDSGISYDKMIFFGLEYKFIAQYLKGELNKNDMF
                     QKLNSAIHAFAKRQMTWFRKMEREGVVINWIDSPDFNKAKALIEGSQ"
     gene            complement(2136..>2327)
                     /locus_tag="VF870_02685"
     CDS             complement(2136..>2327)
                     /locus_tag="VF870_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251115.1"
                     /translation="NTTIKPEMIKDVFNPHFSYNGNPSTGLSLAISKFLIEAMHATFE
                     LHIEDSGTNYRVSIPVSML"
BASE COUNT          723 a          380 c          351 g          873 t
ORIGIN      
        1 ttttggaatt ttaattaatg aatttttgtt taccatattg tcattccgaa cttgattcgg
       61 aatctcttgt tcacttccta ttctttcgaa agaacctgaa acaagttcag gttgacttag
      121 tgacatatca taaaatattt ccagccaggt ctttagaaca acaaaacaat taggatcgta
      181 aagcaaatat tcgtttcgtt catttgataa atatctatct acacttcttc cggtaccaaa
      241 aggtacgcgc cacgaacctc ttttagatgg atgaacaaat gtagatttaa tttctccaat
      301 cggataaatc tttgccagtt tctcaagaaa ataaaaatct tctgcagctt ttcgtttatt
      361 cattccttca acttttacat aagcttctgg agtacacaac atagtggagc ctattgtgtg
      421 aaatgcataa tctgattttg caaaattcaa acctaaaaca taataacgca aaaatatttc
      481 gtaacagatt attgcttttg tttcttcatc attgctgcta atatcgtggg aaaaatttac
      541 aacagcagct tcataattat ttctattaaa atcttgtgaa attttggaaa gataatttgt
      601 atcaacaata caatctgcat cagtgcagat cattattttt ttattgagtg agaggtaatc
      661 aaattttgtg agagctaaat ccatcccgat ttttcttgca agaccgacac cgccattttt
      721 atcatccatt tcttttccat cagtgcaagc atctatggat gaaatattta ctttagattt
      781 tatttttctc agataagcta atgttttttg attatcattt ttaacttcgt ccgatgatga
      841 aagactatta tttacaacaa ttaatagaag tgtgtttagt agttcaagtt catcattctg
      901 ttctaaggat ttaattagtt caggaagatt atttgattca gcaattgatg gtacaataat
      961 aattttttga aaatattttt gatggtcatt ttcaatcttc catttattgg aggatctttt
     1021 gtttaagtaa gtagatattt tatcggggat tagcatttat aaaaatcgtt ataatttatt
     1081 tttcttaaat aggtgttgta gattgcttcg ctctttcaaa gggatgccta tggcagatga
     1141 cttgctcgca atgacttatt gtgagccttc aatcaaagct tttgctttat taaaatccgg
     1201 ggaatcaatc caattaataa caacaccttc acgttccatc tttctgaacc atgtcatttg
     1261 tcgttttgca aaggcatgga ttgcagaatt taatttttga aacatatcat ttttatttag
     1321 ctcgccttta agatattgag caataaattt gtattccaat ccaaagaaaa tcattttatc
     1381 ataacttata cctgaatcca taagcgattt tacttcctca atcattcttt cttcattaag
     1441 tcgttttttt aaacgtgcag ttattcgttt cttaatttca tcacgcgaaa gatagatgcc
     1501 aatatttagt gagttgaatt ttttattact gtcatttcga aggagtgaaa cgactgagaa
     1561 atctttttct tttttattta cagatttctc tcgcgactgg tcgagatcga tataactatt
     1621 agattttgat actgcaattg cttttacgat tctttctctg tcattcaaat cagttgaatt
     1681 gtgtaatgat ggatttaata cttttaacat cgctctaaga tcatcatcgg aaaatgatga
     1741 taagttttga ttgtaagttt caaaatcagc tttgttaaga ttataatcct gcaggattga
     1801 acttaagtac attcctgttc cgcctacaag aaaggggagt ttgttcctgt tttttatttc
     1861 attaaaagat ttataaaaac ctttctgaaa tgaatacaga ttaaattctt cgcttggatc
     1921 aattatatca attaaatgaa aagggatttg cttaccgttt attgaatatt ccgacaaatc
     1981 ttttcctgta ccgatattca ttccgcgata gacctggcgg gaatcagctg agatgatttc
     2041 tccatcaaaa caatctgcaa gttgaacagc aagttttgtt ttgcccgtag cagtacagcc
     2101 aagaatagta attaaatcat attgcatgcc taaaattaca acatagaaac aggaatcgaa
     2161 acccgatagt ttgttcctga atcctcaata tgtaattcaa atgttgcatg cattgcttca
     2221 attaaaaatt ttgaaattgc taaacttaat cctgtgcttg gattaccatt gtaactgaaa
     2281 tgaggattaa aaacatcttt tatcatttca ggttttatcg ttgtatt
//
LOCUS       DASWBY010000143         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_306016, whole genome shotgun sequence.
ACCESSION   DASWBY010000143 DASWBY010000000
VERSION     DASWBY010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306016"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF870_02690"
     CDS             <1..316
                     /locus_tag="VF870_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251116.1"
                     /translation="GVTDIKTDTEIPTQYSISQNYPNPFNPSTMINYSIPNSSLVSIV
                     IYDLLGREVKTLINSEQNAGNYQVIWNGDNNYGSKVSSGTYFYSIRSSDFHQVRKMIL
                     LK"
     gene            400..>2921
                     /locus_tag="VF870_02695"
     CDS             400..>2921
                     /locus_tag="VF870_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9251117.1"
                     /translation="MFTITKYIKLLPFLLFVLISLIGSFIYAGTTGKIAGKIIDKSTG
                     EPLLGANIILLGTTLGASADIDGNYFLINIPPGEYQVKASMIGYSPITIQKVRVSVDQ
                     TTKLDFELISESIELTDVVVTAERLLVRKDLTSTEETVSGDNISMLPLEDVSSVVNLQ
                     AGVIDGHFRGGRSNEVKYLVDGVAVNDVYSGQSSLSPEINSIEEVQVITGTFNAEYGE
                     ALSGVVNQVTKIAGDKLDGNVSFYLGDYVSSNNDIFTNIDDVNPTNVYNIQGFLSGPI
                     PGIENFAKIFLSGRYNYDDGAIYGQRIFNPPDSSNFSANNSADWYVGSTGDGAYVPMN
                     FHERLSLQAKLALNVGGGKGLVLSGFYQDSKYKDYNHLYKLNPDGDYTKYQKSLLGIA
                     SYTLLLSNSAFIDFSLSGLQSEYNQYVFKDPFEMYTYYNTAGEPIHRYRYVDPARKQD
                     VSGNAFLTGGTENWHFNHTTTSITGKTDFTWQVDNINQIKTGFDLTFHTIKYEDFQII
                     IDASTNYKPKIDSGGFTYNTYQAKPFQFAYYLQDKIEIDYLIVNVGLRFDYFQPDGKY
                     LIDPNNISELDELQPPYPDSVQADATSKSQISPRIGLSYPISDQGAIHISYGHFFQIP
                     PFEYLYLNPNYRIPLTGDYPENIGTTLGNTDLKPQQTIMYEIGLQQALTDELGITITG
                     YYKDIRNLLATEIFIKNEFKKFSRLINKDYGSVQGVTLSFEKRFMGGFGASVDYTFQI
                     AKGSASDPNAAFNNAQSNPPIEVNKQLVPLDWDQTHSLNLTLTLGEPGNYIASAIGKF
                     GSGLPYTPSLQNQRTGLENSETKPIFYNVDVYITKYFELFGK"
BASE COUNT          980 a          476 c          529 g          936 t
ORIGIN      
        1 tggagttact gatattaaga cagatacaga gatcccaact cagtattcaa tatctcagaa
       61 ttatccaaat ccatttaacc ctagtacgat gataaattat tcaattccga atagttcact
      121 tgtgtctata gtgatttatg atttactcgg tcgtgaagtt aaaaccttaa ttaacagtga
      181 acaaaatgca ggtaattatc aggtaatatg gaatggcgat aataattatg gttctaaagt
      241 atcaagcgga acttatttct actcaattag atcatctgat tttcatcaag tcagaaaaat
      301 gattctctta aagtagatat ttatttagcg gggcatcttt tcaggtgccc tgcttatttt
      361 taattaaatt ttcttttcat gtaatattag gtggatttaa tgttcactat tacaaaatat
      421 attaagctac tcccattttt gctttttgtt ctcatctcat tgattggatc atttatttat
      481 gctggaacaa caggtaaaat tgcaggaaaa ataattgata agagcacagg tgaaccgctt
      541 ttaggagcta atataattct cctaggtact acacttggag cctcggctga tattgatgga
      601 aattattttc taattaacat tcctcccggc gagtatcagg ttaaagcatc aatgattgga
      661 tattcaccaa ttacaattca aaaagtaaga gtttcagtcg atcagacaac taaactggat
      721 tttgaattaa tatcagaatc aattgaattg acagacgttg ttgttactgc tgaaagactt
      781 ttagttagaa aagatcttac ttcaacagag gaaacagtca gcggcgataa tatttcaatg
      841 cttccattag aagatgtctc atcagttgta aatctccagg caggtgttat cgatggccac
      901 tttagaggag gaagaagcaa tgaagttaaa tatcttgtcg atggagttgc agttaatgat
      961 gtatactctg gtcaatcatc actttcgccg gagattaata gtatcgaaga agttcaggtt
     1021 ataaccggaa cattcaatgc cgaatatggc gaagctttat caggcgtggt aaatcaggta
     1081 acaaagattg ctggtgataa actagatggg aatgtttctt tttatttagg tgattatgtc
     1141 agctctaata atgacatctt taccaatata gatgatgtaa accctaccaa tgtctataat
     1201 atacaggggt ttttatcagg tccgattcca ggaatagaaa attttgcgaa aattttccta
     1261 tccggaagat ataactatga tgacggggcc atatatggtc aaagaatttt taaccccccg
     1321 gattcatcaa atttttctgc aaataattct gcagattggt atgtcggctc tacgggagat
     1381 ggagcatatg taccaatgaa cttccatgaa agattatctt tacaggcgaa gctagcatta
     1441 aatgttggcg gcggtaaagg gttagtgctt tctggttttt atcaggattc taaatacaaa
     1501 gattacaatc atttatataa gttaaatcct gatggtgatt acactaaata tcaaaaaagt
     1561 ttgttaggga tagcaagtta tactttgtta ttaagtaatt cggcttttat agatttttct
     1621 ttgtcgggtt tgcaatcaga gtataatcag tatgttttta aagatccatt tgaaatgtat
     1681 acatattaca atactgcggg ggaacctatt cacagatatc gttatgttga tccagccaga
     1741 aaacaggatg ttagcggcaa tgcatttctt accggcggta cagagaactg gcattttaat
     1801 catactacga caagtattac cggtaaaacg gattttacct ggcaggttga taacattaat
     1861 caaattaaga ccggatttga tcttacgttt catactataa aatatgaaga ttttcaaatt
     1921 ataattgatg catcaacgaa ctataaacca aagatagatt caggcgggtt tacttataat
     1981 acatatcaag caaaaccatt tcagttcgca tattaccttc aggataaaat tgaaatcgat
     2041 tatctgattg ttaatgttgg tttaagattt gattactttc agcctgatgg aaaatatcta
     2101 atcgatccga ataatataag tgagctggat gaactacaac ctccatatcc cgattcagtt
     2161 caagctgatg caacaagtaa atctcaaata agtccaagaa tcggtttatc ttatccaata
     2221 tcagatcaag gtgctattca tatatcttat ggtcattttt tccagatccc tccgttcgaa
     2281 tatttatatc ttaatccaaa ttacaggatc ccgttaaccg gtgattatcc cgaaaatatt
     2341 ggaacaacat taggtaatac agatttaaaa ccgcagcaga caattatgta cgaaatcggt
     2401 ttgcagcaag ctttaacgga tgaattggga ataactataa ccggctacta taaagacatt
     2461 agaaatcttt tagcaacaga gatatttatc aaaaatgaat tcaagaaatt cagcagactt
     2521 attaacaaag attatggttc ggttcaagga gtaactctat catttgaaaa aagatttatg
     2581 ggtggttttg gtgcctcagt tgattataca tttcagattg ctaagggaag cgcatcagat
     2641 ccaaacgcag catttaacaa tgctcaatca aatccaccaa tcgaagttaa caaacagtta
     2701 gtgcctttag actgggatca aacgcattcg ttaaatctga ccttaacttt aggagaaccc
     2761 ggtaattata tagctagtgc aatcggaaaa tttggttcag gattacccta tactccatcg
     2821 ctgcagaatc aaagaacagg tctggagaat agtgaaacca aacccatttt ttataatgtc
     2881 gatgtttata taacaaagta ttttgaatta ttcggaaaac a
//
LOCUS       DASWBY010000144         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_42793, whole genome shotgun sequence.
ACCESSION   DASWBY010000144 DASWBY010000000
VERSION     DASWBY010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42793"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1673)
                     /gene="malQ"
                     /locus_tag="VF870_02700"
     CDS             complement(144..1673)
                     /gene="malQ"
                     /locus_tag="VF870_02700"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX9251118.1"
                     /translation="MKFERSAGILLHPTSLPGKYGIGDLGYDAYKFIDFLEKAGQKLW
                     QVFPLGPTGYGDSPYQCFSAFAGNPNLVSPDKLLEDDLLSDHDLHNIPKHDPTSIDYG
                     EVIEYKKSLLKKAFSNFKHHQEKFEKDFNKFCKENKSWLDDFAFFMSAKEAHGGIVWK
                     DWDEGLVLRKKKSLDEWKKKLEDDILYQKFVQFIFFKQWHEIRAYANNKGIKIIGDMP
                     IFIAYDSADLWSNKELFTVDEKGNLETVAGVPPDYFSATGQLWGNPLYRWKEMEKDDF
                     LWMRNRFSQLFTMVDIVRIDHFRGFEAFWEIPGGAPTAEKGKWVKAPGHKLFKSIKKH
                     LGDVPILAEDLGVITPAVEKLRDDFEFPGMKILQFAFGTDMESKFLPHNFVQNCVVLT
                     GAHDNDTTRAYFEKAKHSDHHIYDHVHKYLNYFGDDIVYELIRLAYRSVANIVIVPMQ
                     DILNLGGEARMNFPGKLGGNWMWRFTWDQIKDDLHLKYFGLAQLYERPPKPKKEETTG
                     V"
     gene            complement(1764..>2250)
                     /locus_tag="VF870_02705"
     CDS             complement(1764..>2250)
                     /locus_tag="VF870_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX9251119.1"
                     /translation="TGFAGGLFFMYQFSDMFAIQPEAYYTMKGATDNGTIEGYSYDAE
                     MKLDYIEVPLLLKFLIPIKDSGVKPAIFAGPSIGFNMSAKTKVEAGGQTFEQDMEDVA
                     STDFGLVFGGGVGFPVGNGELGLDIRYILGLSTIDDSAAKADVKNGVINFNLYYGFNL
                     K"
BASE COUNT          716 a          465 c          359 g          710 t
ORIGIN      
        1 caattcaaac tccttttaaa ttttcactgg tttgattcaa tattttcttt gaggattcag
       61 tctaattatt atgccggact ttatccgtcg ttgatttttt aagaatcata tatttcccgc
      121 atcaagtgcg gaatggcaaa aaattaaacc cctgttgttt cttctttctt tggctttggc
      181 ggccgttcgt aaagctgggc taatccaaaa tatttcaaat gaagatcatc cttaatttga
      241 tcccatgtaa agcgccacat ccagtttccg ccgagttttc cgggaaaatt cattcgtgct
      301 tcaccgccaa gatttaaaat atcctgcatt ggaacaatga caatatttgc cactgatctg
      361 taagcaagcc ttatcaattc gtaaacaatg tcatctccaa agtaatttaa atacttatgt
      421 acgtgatcat aaatgtgatg atctgaatgc tttgcctttt caaaataagc gcgggtagta
      481 tcattgtcat gcgcaccggt aagcacaaca caattttgta caaagttatg aggcaaaaat
      541 tttgattcca tatcagttcc aaacgcaaac tgtagaattt tcattcccgg gaattcaaaa
      601 tcatctctaa gtttttcaac tgcgggagtt ataacaccaa gatcttcagc aagtattgga
      661 acatcaccaa gatgcttttt tattgattta aataatttgt gtccgggagc tttaacccac
      721 ttgccttttt ctgctgttgg tgcaccaccc ggaatttccc agaaagcctc aaatccccta
      781 aagtgatcaa tgcgaacaat atcaaccatt gtaaacaatt gtgaaaaacg gtttctcatc
      841 caaagaaaat catctttttc catttctttc cacctgtaaa gaggatttcc ccaaagctgt
      901 cctgttgctg aaaaataatc cggaggaacg cctgcaacag tttcaagatt tcctttttca
      961 tcaacggtaa ataattcttt atttgaccaa agatcagcgc tatcataagc aataaatatt
     1021 ggcatatcgc cgattatttt tatgcctttg ttgtttgcat aagctcttat ttcatgccat
     1081 tgcttaaaga aaataaactg aacaaatttt tgatacagaa tatcatcttc taatttcttt
     1141 ttccattcgt ctaaagattt tttcttgcga agcaccaggc cttcgtccca gtctttccac
     1201 acaattccgc catgggcttc tttggccgac ataaaaaaag caaaatcatc caaccaggac
     1261 ttattctctt tgcagaactt gttaaaatcc ttttcaaact tttcctgatg atgtttgaaa
     1321 tttgaaaacg ctttttttag taatgatttt ttatattcaa taacttcgcc ataatctatt
     1381 gaggttggat cgtgttttgg aatgttgtgc aaatcatgat cactaagcaa atcatcttcc
     1441 agtaatttat ccggactaac gagatttgga ttaccagcaa aggcggaaaa acattggtag
     1501 ggcgaatctc catacccagt tggaccaaga ggaaaaacct gccatagttt ttgtccggct
     1561 ttttctaaaa aatcaataaa cttataagca tcataaccaa gatcaccaat tccatattta
     1621 ccgggaagag atgtgggatg aagtaaaatg ccagcgcttc gttcgaattt cattatataa
     1681 cctttaaaaa atgtggggga aaaataagga gagagtgagc aaaaaaaaat cctgctcctt
     1741 ttacagagca ggatttaata agattatttt aaattaaagc cgtagtaaag attaaaattt
     1801 ataacaccat tttttacatc agctttagca gctgaatcat ctatcgttga taagccaaga
     1861 atatatctga tatcaaggcc caactctccg ttaccaaccg gaaaacctac tccaccacca
     1921 aaaaccaatc caaaatcagt tgaagcaaca tcttccatat cttgttcaaa agtttggccg
     1981 ccagcctcta cttttgtttt agcagacatg ttaaatccga tggaaggacc tgcgaaaata
     2041 gccggtttaa caccagaatc tttaattggg attaagaatt ttaataacaa aggaacttcg
     2101 atgtaatcaa gtttcatctc agcatcatat gaatatcctt caatagtccc attgtcagtc
     2161 gctcccttca ttgtgtagta agcttctggc tgaattgcga acatatcgct aaactgatac
     2221 ataaaaaaca aaccgccagc aaaaccagta
//
LOCUS       DASWBY010000145         2694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_80611, whole genome shotgun sequence.
ACCESSION   DASWBY010000145 DASWBY010000000
VERSION     DASWBY010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80611"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VF870_02710"
     CDS             <1..510
                     /locus_tag="VF870_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854979.1"
                     /note="catalyzed the formation of 2-ketoglutarate from
                     2-hydroxyglutarate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX9251120.1"
                     /translation="EFLVNNLIYPVPDINFPFLGVHFTRMINGGVEAGPNAVLAFKRE
                     GYKKTDFSLSDILEMKLYPGFWKMAAKFYKMGFQEFRRSFSKELFVKSLQKLIPEIQS
                     DDIEAGSAGVRAQALERDGKLVDDFRIVEAQRMVHVLNEPSPAATASLSIGKTISEMV
                     IKRFNEEVN"
     gene            517..1560
                     /locus_tag="VF870_02715"
     CDS             517..1560
                     /locus_tag="VF870_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX9251121.1"
                     /translation="MKQDLLNTISNSLDKSDLKEIKKDIFNLLDFVKKNKGKNIDMGN
                     GYASIPKEFLLGELNQISETHTIERTKYYIKRLKKSLTETKQSKINDLNLNRWKEYDD
                     IITDSLWMLNKRDNSGVHHAGYWGNFIPQIPNQLLRRYTKKGEWVLDAFLGSGTTLIE
                     SQRLGRNAVGIELSKNVLKQTKKNIAKEKNQFDIKLEFINGNSSSVDLKSVLPSLQIK
                     SFQFQIFHPPYWDIIKFSDNKSDLSNAKSIDEFLNGFGTVLDNCLPLLDKKRYAAVVI
                     SDKYSGGEWIPLGFYVMQEVLKRGMILKSTIVKNFDETTAKRNQKELWRYRALAGGFY
                     VFKHEYIFIFQKK"
     gene            1557..2201
                     /locus_tag="VF870_02720"
     CDS             1557..2201
                     /locus_tag="VF870_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251122.1"
                     /translation="MRRSLNKIESELKKRLVYPYKWGQKQNDNFDKQTNFIYHTFLFE
                     DLLEEIDNRFKSETDFDIYFNYTINRWYNFWSAQAVENIFCSLPNVKPALDSKDRLVD
                     FTIDGEAFDHKTSIFPKNFPYKIDEAIKRTDELIKWLYKHQSQQQRKHLKNRLFVVLY
                     SKDGEHWRLKSEIAWLKYRIEKYMLGFNPNYLLKYNFEKGKETISDVIWAIRDK"
     gene            2361..>2694
                     /locus_tag="VF870_02725"
     CDS             2361..>2694
                     /locus_tag="VF870_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251123.1"
                     /translation="MKKLLLFSITLIISITFLSCVDSGKDESDKTGKESTSEFQKRYG
                     IKSGVIEYIITGSQEGTKTLYFDNWGMRQAEYTRSILSVGGFTKPLNLVNIIDGEYQY
                     MINIDQNSG"
BASE COUNT         1007 a          363 c          490 g          834 t
ORIGIN      
        1 gaatttcttg taaataatct tatctatccc gttccggata ttaattttcc ttttcttgga
       61 gtccatttta ccaggatgat aaatggcgga gttgaagccg gaccaaatgc cgtgcttgct
      121 tttaaacgtg aaggctataa aaaaacagat ttttctctta gtgatatttt agaaatgaaa
      181 ctctatcccg gcttctggaa aatggcggca aagttttata aaatgggatt tcaggagttc
      241 agaagatctt ttagtaaaga gctgtttgta aaatcacttc aaaaattgat tccggaaatt
      301 caatctgatg atattgaagc aggcagtgcg ggagttagag cacaagcatt ggaacgggat
      361 ggaaaacttg ttgatgattt tagaattgtt gaagcccaaa ggatggttca cgttttaaat
      421 gaaccttctc cggcagcaac agcatcttta agcattggaa aaacaatttc tgaaatggtt
      481 attaaaagat ttaacgaaga agtaaattaa tattaaatga aacaagattt attaaataca
      541 atttcaaatt cacttgataa atcagatttg aaagaaatta aaaaggatat ttttaatttg
      601 ttggattttg ttaaaaagaa taaaggcaaa aacattgata tgggaaatgg gtatgcatct
      661 attcctaaag aatttttgct tggtgaattg aatcagattt cagaaaccca cacaatcgaa
      721 cgaactaaat attatataaa acgattgaag aaaagtttaa cagaaaccaa acaaagcaaa
      781 ataaatgatc taaatctgaa ccgatggaaa gagtatgatg atattattac agatagtctt
      841 tggatgctaa acaagcgcga taattcggga gttcatcatg cagggtattg gggaaatttt
      901 attccgcaaa tcccgaatca acttttaaga cgctatacaa agaaaggcga gtgggtttta
      961 gatgcatttt taggcagcgg aacaacgtta attgaatctc aaagacttgg cagaaatgca
     1021 gttgggatag agctttccaa gaacgttctg aaacagacaa aaaaaaatat tgccaaagaa
     1081 aaaaatcaat ttgatataaa acttgagttc ataaatggaa atagttcatc tgttgatcta
     1141 aagagcgttc taccatcatt gcaaataaaa tcatttcagt ttcagatttt tcatccacct
     1201 tactgggaca taattaagtt tagtgataat aagtctgatc tgtcaaatgc aaaatcgatt
     1261 gatgaatttt taaacggttt tggaacagtt ttagataatt gtcttccact tcttgataaa
     1321 aaacgatatg ctgccgtagt tataagcgat aaatactctg gtggtgaatg gattccgctt
     1381 ggcttttatg ttatgcagga agttttgaaa cgcggaatga ttttgaaatc aacaatagtt
     1441 aaaaactttg atgaaactac tgcaaagcgt aatcaaaaag aattgtggcg ctatcgtgca
     1501 ttagcaggag gattttatgt ttttaaacat gagtacattt ttatttttca gaaaaaatga
     1561 ggcgttcatt aaacaaaata gaatccgaat taaaaaaaag acttgtatat ccctacaaat
     1621 ggggacaaaa gcaaaacgac aattttgata aacaaaccaa ctttatttat cacacatttt
     1681 tatttgaaga tttactagaa gaaattgata atcgatttaa atcagaaaca gattttgata
     1741 tttactttaa ctatacaata aacagatggt ataatttctg gtcggcacaa gctgttgaaa
     1801 atatattttg ttctctccca aatgtaaagc cagcattgga tagcaaggac aggcttgttg
     1861 attttactat tgatggtgaa gcgtttgatc acaaaacttc aatatttccc aaaaactttc
     1921 cgtataaaat tgatgaagca ataaaaagaa ctgatgaatt aattaaatgg ctgtataaac
     1981 atcaatccca acagcagaga aagcatttaa agaacagatt atttgtagtt ctttattcaa
     2041 aagatggtga acattggagg ttgaaatcag aaattgcctg gcttaaatat agaatcgaaa
     2101 aatatatgct aggatttaat ccaaattatt tattaaagta taactttgaa aaagggaaag
     2161 aaacaatttc tgacgtaatt tgggcaataa gagacaaata ataaatttaa tagagtgtcg
     2221 cacttcgact tcgctcagtg tgatatgcta atttttcacg acaaaaatga caataaattt
     2281 ttattacttc taaattttaa ttgaagtaag attacacttt acttattttc aattagtgaa
     2341 aaacataaag aaataagaca atgaaaaaat tattattatt ttcaattact ttgatcatct
     2401 caataacttt tttaagctgc gttgatagcg gtaaggatga atctgataag acaggtaagg
     2461 aatcgacttc cgagtttcag aagagatatg gaattaaatc cggagtgatt gaatacataa
     2521 ttacgggttc gcaggaaggg acaaaaactc tttactttga taactgggga atgagacaag
     2581 ctgaatacac aaggtctatc ttaagtgtgg gcggatttac aaaaccttta aatcttgtaa
     2641 atattattga tggcgaatat cagtatatga ttaatattga tcagaatagc ggaa
//
LOCUS       DASWBY010000146         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_7519, whole genome shotgun sequence.
ACCESSION   DASWBY010000146 DASWBY010000000
VERSION     DASWBY010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7519"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1379
                     /locus_tag="VF870_02730"
     CDS             165..1379
                     /locus_tag="VF870_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9251124.1"
                     /translation="MLLTLAWRNIWRNKKRSLIIVAAIAVGLCCGLFASATMFGMWDS
                     MINTTIDRDLGHFQIHTKEFEDEKLITDTIPDFENVVAEIQKSKDVSGVSSRILIEGM
                     ASSATISNGVRIIGISPEDEEKVTSIHKQLIKGSYFGEEWKNQIVIGERLADNLGIKE
                     RSKIVLSFQGMDGSIIYGAFRVTGIFKTESSVFDKANVFVKENDLANLLNSKIISNEI
                     IVRLSSVQQLDSVYIPIKNTYPELSVKNWGELAPELKLFYDMLILEMYIFVGIILFAL
                     LFGITNTMLMSVMERVREFGVLMAVGMKRSRLFLMIMLETITLSFIGGIVGLLFALGL
                     IEYYSRAGINLSAFTEGLSQWSLGTQLYTYLPFSFYPPLALMILSTSVIAALYPALKA
                     IKLNPATAIRTF"
     gene            1456..1866
                     /locus_tag="VF870_02735"
     CDS             1456..1866
                     /locus_tag="VF870_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX9251125.1"
                     /translation="MTKKYLNELTYQIIGAAIEVHKELGPGLLESVYHRCLKQELFLR
                     KIKFYTEQVVPVNYKGIELDDELRCDLIIEDAIVVELKSVETLSPIFEAQILSYMKLL
                     QKPKGILINFNCLNIFKEGQKTFVNELFQELPEE"
BASE COUNT          617 a          266 c          388 g          601 t
ORIGIN      
        1 agcaattact gagtggggat ttgaaccaat cttaccattt gcggtttatc cgggaatgtt
       61 cttcgcacaa atctggacag tgctggcaat tgcaatggtt tctgttattt atccggtctt
      121 gttcattaga aaattaaaac cagtagaagc gcttagaggc tgatatgtta ttaacacttg
      181 cctggagaaa tatctggaga aacaagaaac gcagtttgat tattgttgcg gcaatagctg
      241 tgggtctgtg ctgcggttta tttgcaagtg caacaatgtt tggaatgtgg gattcgatga
      301 tcaacaccac tattgacaga gacctgggac attttcaaat tcataccaag gaatttgaag
      361 atgaaaaact aattacagat acaattccag attttgagaa cgttgtcgca gaaattcaaa
      421 aatcaaaaga tgtcagcggg gtgtcatcca gaattctgat tgaaggtatg gcatcttctg
      481 caacaatttc taatggagta agaataatcg ggatcagtcc tgaggacgaa gaaaaggtta
      541 cttcaataca taagcaatta attaaaggga gctatttcgg agaggaatgg aaaaatcaaa
      601 ttgttatcgg agagagactt gcagataatc ttgggataaa agaaagatct aaaattgttt
      661 tgagttttca ggggatggat gggtcaatta tttacggagc atttagagtt acgggaatat
      721 ttaaaacaga atcttctgtt tttgataaag cgaatgtatt tgtaaaagag aatgatctgg
      781 caaatttgct gaactcaaaa attatatcga atgaaattat tgtgcgttta agttctgtgc
      841 agcaattgga ttcggtttac ataccgataa aaaacactta tcctgagctt tctgtaaaga
      901 actggggaga acttgcgcca gaactaaaat tattttatga tatgctgata ttggaaatgt
      961 acatttttgt tggaataata ttattcgcgt tactctttgg tataacaaac accatgttaa
     1021 tgtctgtaat ggaacgcgtt cgcgagtttg gtgttttgat ggctgtaggg atgaaaagaa
     1081 gccgtctgtt tttgatgata atgcttgaga ctattacttt gtcatttatc ggcggtattg
     1141 tcgggttatt gtttgcattg gggctgattg aatattatag tcgtgcaggg attaatcttt
     1201 cagcatttac agaaggactt tcacaatgga gtttagggac acagctatat acttatttac
     1261 ctttttcatt ctaccctccc cttgcgttaa tgattctgtc cacatcggta attgcagctt
     1321 tgtacccagc gcttaaggca atcaaattaa atcctgcaac agcaataaga actttttaga
     1381 gcatggataa accacataga cacatagaaa acattagatt tacaatatgt taatattttt
     1441 aggaagataa ttgaaatgac aaaaaaatat cttaatgaat taacatatca aataattgga
     1501 gcagcaattg aggttcacaa agaattaggt cctggtttac ttgaaagtgt ttatcataga
     1561 tgccttaaac aagagttatt tctgcgaaag attaagtttt atactgaaca agttgtccct
     1621 gtgaactata aaggaataga gcttgatgat gaacttagat gtgatctcat tattgaagat
     1681 gcaatagttg ttgagcttaa atcagttgag actttatctc caatttttga ggctcaaata
     1741 ctttcttata tgaagttgct gcaaaagcca aaaggaattc taataaattt taactgctta
     1801 aatattttta aagaaggtca aaaaactttt gttaatgaat tattccagga attacctgag
     1861 gaatgaaaaa at
//
LOCUS       DASWBY010000147         4412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_80675, whole genome shotgun sequence.
ACCESSION   DASWBY010000147 DASWBY010000000
VERSION     DASWBY010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80675"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VF870_02740"
     CDS             <1..593
                     /locus_tag="VF870_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251126.1"
                     /translation="VTSFEVINAYKIKVSFNFSVDELSAQNTNNYIFTPDNKVTSVSI
                     DQGDSKTIYLDLTNQKPIGSIGKEYVLRITNLQSDGSSGNIPINSGAGSYLVLSSFAK
                     NLSDVYVYPNPTKAGTEIITFANLPQRAKISIWTIDGILVNELEENDGNGGVDYNLID
                     MSGNKISSGVYFYRIVQLDQSNNEGEEKLGKFAVVR"
     gene            590..1162
                     /locus_tag="VF870_02745"
     CDS             590..1162
                     /locus_tag="VF870_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9251127.1"
                     /translation="MKYSSKEIFTISNGISFLRFLLVIPLWFLLDNYELESVRYITFA
                     LCVFAAATDMLDGFLARKLNQITEVGKIIDPLADKAAMAVIVVKLFLIGEISTFFFLT
                     IILRDLIIFIGGLFVANRLGRVLPSNKLGKITVLFIGSVVLLILLGINRESFIYHFFY
                     YSSIVLMFTSLFAYIYRAFEFIKKKKNESI"
     gene            1149..2078
                     /gene="ftsY"
                     /locus_tag="VF870_02750"
     CDS             1149..2078
                     /gene="ftsY"
                     /locus_tag="VF870_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX9251128.1"
                     /translation="MSLFKNFNLDKLKSGLSKTRDKIVNSITETLTGKAVIDDLTLDQ
                     IEEVLLGSDIGFLTVERIIESVKRNLKSEKDRSGENIIEIVKQELTAVLSVSNSNGKV
                     LSGKKPYIILIVGVNGVGKTTTIGKLANNYKKIGKKVIVGAADTFRAAANEQLEIWAK
                     RADVDIIQSLKGTDPSSVVFETVKKSIDENYDIVLIDTAGRLHNKTNLMNELDKIRRV
                     IKKLLPDAPHETLLIVDGNTGQNAILQAEEFSKVTNVSGLVITKLDGTAKGGVVFQIV
                     SKQNIPVKFIGVGEGIDDLQEFDSKAFVEAIFS"
     gene            complement(2347..>4412)
                     /locus_tag="VF870_02755"
     CDS             complement(2347..>4412)
                     /locus_tag="VF870_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX9251129.1"
                     /translation="KKNIVVFFAGFKKYLEKIAFPEYTFFSVYAIIIGIAAGLSAVFF
                     HLSIDFFNKLFFEKTKEGLYFLGAAAVILLPAIGMFIQYIMIKAAPDISKKRGVLEII
                     KSVATKSGYIPFRTTLFHFFAPVISIGSGGTVGPEGPAAQIGGGVASKLATILKVSDQ
                     RRRIFTAAGSGAAIAAIFNSPLGGVFFALEIILLNDFHTPTFSALILASVTASAISRI
                     FLGNRSVFTFDVPHIGEYQYFYLFILLGLFCGFIAVMFLKFDNVTSRFFKKSIIKKIP
                     LWIVMVTVGLLVGVSGYFYIDIFGIGYSGINNILSNSNTWQVVLVLFALKFILVPLTL
                     NSGGFGGTFAPSLFMGACAGYLFSFAVTSLFGLPTDSTTYILVGMGAMLGGINSIPLT
                     AILMIFEMTREYSFILPLMLSVIISSTIVQIVNKGAYHVKKLEKQGFRLTGGKETSVL
                     KSISVESVMQSDPITINENASLEFVVSKLVESQHHIIYTTDSDGNLSGAISETQIRPL
                     ITEFDSLKGSLIASDIADNRITTIIRDQDLDYVLKVLTKGDLEELPVVSDSDDKRIIG
                     TISRHDILSTYNKESLKSDLAEGLSREINTLAEAKISRIADGYAIIEKRPHHNFIGKT
                     LSELKLRNNYGLEVLMIKKSKELFDEGENESRLVMPGHNYKIEADDILVLFGTDEKIA
                     ITKDW"
BASE COUNT         1533 a          706 c          743 g         1430 t
ORIGIN      
        1 atgttacatc gtttgaagta attaatgctt acaaaataaa agttagtttt aattttagtg
       61 ttgatgaatt atctgctcag aatacaaaca attatatttt tacacctgat aataaagtca
      121 cttccgtatc aattgatcag ggtgattcaa aaacgattta tttagattta acgaatcaaa
      181 aaccaattgg ctcaataggg aaggagtatg ttcttagaat tactaatctt cagtcagatg
      241 ggtccagcgg taatattcca ataaatagcg gcgcaggcag ttatttggtt ctttcctcat
      301 tcgcaaaaaa tctttctgat gtttacgttt atccaaatcc tacaaaagca ggaacagaaa
      361 taattacatt tgctaatctt ccgcagagag caaaaatatc aatatggaca attgatggaa
      421 ttcttgtcaa tgagcttgaa gaaaacgatg gtaatggcgg agttgattat aatcttattg
      481 atatgagcgg taataaaata tcgagcggcg tttattttta tcgcatagtc cagcttgatc
      541 aaagcaataa cgaaggagaa gaaaaacttg gcaaatttgc agtggtaaga tgaaatatag
      601 cagtaaagaa atatttacaa tttcaaatgg gattagtttt ctaaggtttt tgcttgtaat
      661 tccgttatgg tttttattag ataattacga attggaatct gtaaggtaca ttacttttgc
      721 actttgtgtt tttgccgctg caacagatat gcttgatgga tttcttgcaa gaaagttaaa
      781 tcaaattacg gaagtaggaa aaataattga tccgcttgca gataaagctg caatggctgt
      841 aatagtggtt aaattattct tgattggtga gatttctaca ttcttctttt taactattat
      901 cctaagggat ctaataattt ttattggagg acttttcgtt gccaacagac ttggcagggt
      961 tcttccatca aacaaactcg ggaaaattac agttttattt atcggctctg tagtactttt
     1021 aattttatta ggcattaaca gagaaagttt tatttatcac tttttttatt attcaagtat
     1081 agttttaatg ttcacttctt tgtttgcata tatttacaga gcctttgaat ttatcaagaa
     1141 aaagaaaaat gagtctattt aagaatttta atttagataa attaaaatca ggtttaagca
     1201 aaacacggga taagatagtt aattccatta ctgaaacctt aacgggcaaa gcagtaattg
     1261 atgatttaac cttagatcag attgaagagg tacttttagg ctctgatatt ggatttctta
     1321 cggttgaaag aattattgaa tcagtaaaac gaaatcttaa gtctgaaaaa gatcgttctg
     1381 gtgaaaatat tattgagatt gttaaacagg aattgacagc ggttttatct gtttcaaatt
     1441 ctaatgggaa agtgttatca ggcaaaaagc catatattat tttaatcgtt ggcgtaaatg
     1501 gagtagggaa aacaacaacg ataggaaaat tagccaacaa ttataaaaag attggtaaaa
     1561 aagtaattgt gggcgcagca gatactttcc gcgctgctgc aaatgaacaa ttagaaatat
     1621 gggctaagcg ggcagatgta gatattattc aaagtctaaa gggcactgat ccttcttctg
     1681 tagtttttga aacagttaaa aaatcgattg atgaaaacta tgatattgta ctgatcgata
     1741 ctgccggaag acttcacaat aagactaatt tgatgaatga acttgacaaa atcaggcgcg
     1801 taattaaaaa gttgctccct gatgctcccc acgaaacact attaatagtt gatggaaata
     1861 ccggacaaaa tgcgatattg caagccgaag aattttctaa agtaacaaat gtttccggac
     1921 ttgttattac aaaattagat ggtacagcaa aaggcggtgt tgtttttcag atcgtatcta
     1981 aacagaatat tcctgttaaa tttataggtg ttggtgaagg gattgatgat cttcaggaat
     2041 ttgactcgaa agcatttgtg gaagctatat ttagttagat aaaattatgt taatgaaaaa
     2101 accacgacac agagtttttg actatccgcc aaggttttat aaacctcagg aagatccaca
     2161 ggaaaaatta aaaaagcgat tgggttttca aagttataga aagtttaaac atagaaagaa
     2221 gagtccgatt atctggatag tgtttatcat tgctataatt tttataataa taaaattgag
     2281 cggtaagtga taaaaaatga aataagttag tttacataat atacattatc ggacaaatct
     2341 aattttctac caatctttag taatagcgat tttttcatca gttccaaata ataccaatat
     2401 atcatctgct tcgattttat aattatgccc cggcataaca agtcttgatt cgttttcacc
     2461 ctcatcaaaa agttctttag attttttaat cattaaaact tccaggccat aattatttcg
     2521 tagttttaat tcagataaag ttttcccgat aaaattatga tgaggtcgtt tctctataat
     2581 tgcataacca tcagcaattc tagatatttt tgcttcagct aaggtgttta tttctctgct
     2641 taatccttct gctagatcgc tcttcaaact ttctttatta taggttgata aaatgtcgtg
     2701 tctggaaatc gtaccaataa ttcttttatc atcactatct gataccacag gtaattcttc
     2761 taagtcacct tttgttaata ctttaagaac ataatctaaa tcttgatctc taataatggt
     2821 cgtaatacga ttatctgcaa tatcacttgc tataagcgat ccttttaatg aatcaaattc
     2881 agtaattaat ggtcttatct gtgtctccga aatggcacca gaaagatttc catcactatc
     2941 tgttgtataa attatatgat gctgcgattc caccaacttt gatactacaa actctaaaga
     3001 ggcattttca ttaattgtta taggatcact ttgcattaca gattctacac tgattgattt
     3061 taacacactg gtttcttttc caccagttaa tctaaatcct tgcttttcca atttctttac
     3121 gtgatacgca cctttattta ctatctgaac gattgtcgag cttattatta ccgatagcat
     3181 cagcggtaaa ataaatgaat attcacgtgt catttcaaaa atcattaaaa tagctgtaag
     3241 aggaatcgaa ttaatgccgc caagcattgc tcccattcca accaggatat aagttgtgga
     3301 atctgttggt aacccaaaaa gactagttac ggcaaaggaa aacaaatatc ctgcacaggc
     3361 gcccataaat aaagatggag caaaggttcc cccaaatcca ccggaattta gtgttagagg
     3421 gaccagtata aatttcagag caaataaaac aagtacgacc tgccaagtat ttgaattact
     3481 aagaatatta ttaatccctg aatatccaat cccaaaaata tctatataaa agtaaccact
     3541 aactccgaca agtaatccga cagttaccat aacaatccaa agcggtattt tttttattat
     3601 actttttttg aagaatctcg aagtaacatt atcaaatttt aaaaacatta ctgcaataaa
     3661 gccgcagaac agtcctaata aaatgaacaa ataaaaatac tgatattcac caatgtgagg
     3721 tacatcgaat gtaaaaactg atctgttgcc taaaaatatt cttgagattg cgctggcagt
     3781 tacagaagcc agaataagag cagaaaatgt tggagtatga aaatcgttaa gtaaaattat
     3841 ttcaagagca aaaaataccc cgcctaatgg agagttaaat atagcagcga ttgcagcacc
     3901 ggaaccagcg gcggtaaaaa ttcttcttct ttgatcggag actttaagta ttgttgccaa
     3961 tttacttgca actccacccc caatttgtgc tgcaggtcct tcaggtccaa ccgttcctcc
     4021 agaaccgatg gaaattactg gtgcaaaaaa gtgaaataaa gtagttctaa atggaatata
     4081 accacttttg gttgccacag actttatgat ttccaagact ccccttttct tagagatatc
     4141 aggtgcggct tttatcatta tgtattggat aaacattcca atagcaggca aaagtattac
     4201 agctgcggca cctaaaaaat ataacccttc ttttgtcttt tcaaaaaata gcttattaaa
     4261 aaaatctatg gatagatgaa aaaatacagc agaaagacct gccgctatgc caataattat
     4321 tgcgtaaaca ctaaaaaaag tatattccgg aaaagctatt ttctctaaat attttttaaa
     4381 gcctgcaaaa aaaacaacaa tatttttttt ct
//
LOCUS       DASWBY010000148         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_531318, whole genome shotgun sequence.
ACCESSION   DASWBY010000148 DASWBY010000000
VERSION     DASWBY010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531318"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1556)
                     /locus_tag="VF870_02760"
     CDS             complement(81..1556)
                     /locus_tag="VF870_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251130.1"
                     /translation="MTDMASLIPGFEYDIFISYRQKDNKYDNWVSEFVDNLKKELEAT
                     FKEEISVYFDINPHDGLLETHDVDESLKEKLKCLVFIPIISRTYCDPKSFAWEHEFKA
                     FVEQASQDQFGLKIKLPNGNVANRVLPIRIYDLDNTDIKLCEAVLGGVLRGVEFIYHS
                     QGVNRPLRQRDDDVTQTIKQSIYRDQINKVANAVKEIILGLKAVPEASVKESEQNLKT
                     VETEKKEGRRGTSDKTLKKTSKRKLLTGVLSILVLISIILIYPKIYNKEKVENSNSGK
                     ILDRAIEFYDFMNRWDNYHGKVHLTTAFPGLGLLNEEIIEIQTKEDFYQSSNISGGNK
                     SVKGIQNGECFIEIDNNRYLGKEQVNKLGKNCTDIQFYKEHHYHHFGLLMELKTSGLI
                     LENKISTIKFQGFNCLALTFTNDPEKVNNVYYNEMNSIIIYLDPSNYSMKGYKITGSR
                     NLYAVCSGILNINGIKMPLCKTYFNSSDNSFLFVDLFSAAN"
BASE COUNT          481 a          312 c          225 g          558 t
ORIGIN      
        1 tccggatttt ggcagtcaac ttatacaggc caacaacagt caggaccaaa ggtttttcca
       61 attaggtaat gtaaatactt ttaatttgca gcactgaaca agtctacaaa caaaaatgag
      121 ttatccgaac tattgaaata agtcttgcat agaggcattt taatgccatt aatgtttaaa
      181 atacctgaac agacagcgta aagattcctt gagccagtaa ttttatatcc tttcattgaa
      241 taattagacg gatcaaggta aataataata ctgttcattt cattgtaata tacattatta
      301 accttttcag gatcattagt aaatgtaagt gctaagcaat tgaatccctg gaatttaatt
      361 gtactaatct tattctcaag aatcagcccc gaagttttta attccatcaa taatccaaaa
      421 tgatgatagt gatgctcctt ataaaactga atatccgtgc aatttttacc taatttattg
      481 acctgttctt tccccagata tcgattattg tctatttcaa tgaaacattc tccgttttgg
      541 atacccttaa cacttttatt gccccctgaa atgttggaag actgataaaa atcttctttt
      601 gtttgaattt caattatttc ctcgttaagg agacccaatc cagggaatgc agttgttaaa
      661 tgaaccttcc catgataatt atcccagcga ttcatgaaat cataaaattc aattgctcta
      721 tctaagattt tcccactgtt tgaattttca actttttctt tattataaat ctttggatat
      781 atcaatatta ttgatattaa aactaaaatg ctcaatacac cagtaagtaa tttacgctta
      841 cttgtttttt ttaatgtctt atcgcttgtt ccccttctac cttctttttt ttctgtctct
      901 acagttttta ggttctgttc actttctttt acagatgctt ctggtactgc tttcaatcca
      961 agaataatct cttttactgc attcgccacc ttattaattt ggtctctata aatagattgt
     1021 tttattgttt gagttacatc atcatcccgt tgccttaaag gacgattaac tccttgagaa
     1081 tggtaaataa actctactcc ccgaagtact cctcccaata ctgcttcaca taacttaata
     1141 tcagtattat ccaggtcata aatacgaata ggtaaaactc tattagccac attcccattt
     1201 ggcaatttaa tcttcaatcc aaattgatcc tgagatgctt gttcaacaaa tgccttgaac
     1261 tcatgctccc aggcaaagga cttcgggtca cagtaggttc tggatatgat cgggataaat
     1321 acgaggcatt tcagcttctc cttcagggat tcgtcaacat catgggtctc cagaagacca
     1381 tcatgtggat taatgtcaaa gtaaacactg atctcttcct taaaagtagc ctctaattcc
     1441 ttttttagat tgtctacaaa ctctgatacc caattgtcat acttattatc cttctggcga
     1501 taactgataa aaatatcgta ctcaaaaccg gggatgaggc ttgccatgtc agtcaatcat
     1561 tgtgataaag ttataa
//
LOCUS       DASWBY010000149         8906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_120138, whole genome shotgun sequence.
ACCESSION   DASWBY010000149 DASWBY010000000
VERSION     DASWBY010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120138"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VF870_02765"
     CDS             complement(<1..386)
                     /locus_tag="VF870_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9251131.1"
                     /translation="MSLDLKEKKLPFGLTDFVPAHKPEIAFALSGGGARGLSQIGVLK
                     ALEEEGIKPDLIVGTSMGSIVGGLYAAGYTVDQLDSIAHQTDWNDLLTLNTQSDRRDL
                     FIDQKVTEDRAIFSLRLDGLNPILPTS"
     gene            complement(505..2352)
                     /gene="yidC"
                     /locus_tag="VF870_02770"
     CDS             complement(505..2352)
                     /gene="yidC"
                     /locus_tag="VF870_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX9251132.1"
                     /translation="MDKQTTIAFILIGAILVLWLYISAPDPNKQPKQKSETTQTTDSV
                     KAVTKDSIAESKTTPEQTTTENVSIFESTIPEEYLVIETQLARFELSNKGANFRKIFL
                     TKFRNWYSANKKGETNFSDYVQLIDYKKGGTYDVSFVSVDGKGINTKDITFNSDKPAG
                     KYKIDKNDSLTINYSFELEPGKTLIKTYTFFGDKYEIKSKISFDGFNNLISNNSYDLI
                     WSNGIRSIERNSVDEANYSNASVYYGDEQVIVNASSVGEKEVKEFRGRVDWLTVRNKY
                     FAAVIIPDDPQKVEGAYIEGTHEKVGKDGIHETYNARLTLPFQNKKIESQSFTLFIGP
                     VDYKELKAYGRNLVKIVDFGSFFGLKFIVRPIAEYILLPLFQFINSFIPNYGVVLIIF
                     SIIIKIVVYPLTKSSYQSMKKMQALQPMIAELKEKFKDDPQKMNKETMKLYSTYGINP
                     AGGCLPMLLQMPIFVALWGMFQSAIDLRQQPFVGWITDLSQPDVIFHLGTKLPLIGIQ
                     EISGLALLMGITTFVQQKMTVKDPKQQALIYMMPIMLTLLFMSFPSGLNLYYFMFNVL
                     SIAQQYYINHKGGTVELVPVANPNKKKGFMSKLMEAAEEQSKSHQKKKK"
     gene            complement(2457..2852)
                     /locus_tag="VF870_02775"
     CDS             complement(2457..2852)
                     /locus_tag="VF870_02775"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX9251133.1"
                     /translation="MKNFSLTRKERVKKKKDFEKIYCSDNILISSDRLIKLHFLFVDS
                     NVAGVKIAAAVSKKAGKAVWRNRVKRIIKEAYRLNKKLLLQKALEKNIQLLLVFSPNR
                     LSEASNKKIYLRDISPGVVELMSKIIQKI"
     gene            complement(2858..3004)
                     /gene="rpmH"
                     /locus_tag="VF870_02780"
     CDS             complement(2858..3004)
                     /gene="rpmH"
                     /locus_tag="VF870_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX9251134.1"
                     /translation="MKRTFQPSNRKRKNKHGFRERMKTKNGRRVLASRRAKGRKKLTV
                     SDEK"
     gene            3570..4985
                     /gene="dnaA"
                     /locus_tag="VF870_02785"
     CDS             3570..4985
                     /gene="dnaA"
                     /locus_tag="VF870_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX9251135.1"
                     /translation="MDNLKFINLETSTSAVNTDANLIWKNCLTRIKENVTLMTYNTWF
                     LPIRPVSFSDNNLKVQLPNQFFWEWIDEHFSTIVKKTIFEVLGENGKLSYIISDEQEQ
                     KEEFVKQSSLINTNVEKTIETKRPEHETNLNPRYKFENFIKGEGNQLARAAAGAISDN
                     PGGTSFNPFFIYGGVGLGKTHLIQSIGNSIIEKFPEKKVIYLSSDSFTVEFVEAIQSN
                     KVNDFSGFYRNMDVLIIDDIQFLMGKEKTQDLFFHIFNTLHQSRKQIILSSDKPPKDL
                     KGLDERLISRFQWGLSADIQPPELEMRIAILKNKAEEYGMLVSTEILEFIAGSITSNI
                     RELEGCLIKLLANASLNSKEITLELAKRTVKEIATDRKINITIDDISKVVCHYLNIPE
                     NKIREKTRKKEIVLARQLAMYFSKELTKSSLKTIGLHFGGRDHSTVIHACGSIEQMRN
                     NDLSMKELIDSIRNKIDLNYS"
     gene            5365..5775
                     /locus_tag="VF870_02790"
     CDS             5365..5775
                     /locus_tag="VF870_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251136.1"
                     /translation="MKKQLLLLFTLAVVSVIVFTSCEPFIENKITVRNEASGDVTLIV
                     AGKTYDVAANSSLVLPDFKKGTFAYSTVYSVPLGVTDASAEGDVSGNFVLNAGTEILL
                     QYTSVTVDSSYTIFGILTSSDDVNRVDPIIDSGN"
     gene            5948..7057
                     /gene="dnaN"
                     /locus_tag="VF870_02795"
     CDS             5948..7057
                     /gene="dnaN"
                     /locus_tag="VF870_02795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX9251137.1"
                     /translation="MEFKLNSKILEKLLAKIIPAVPSRTPMPILENFLFEIKDGSLTV
                     SATDLEIALRSSLNVAAEKNIKMVVPARLLYDIVRSLEDTQVSFLVDSKGKIKITTDK
                     GVYSLSYSPSEDFPEIATVSKEKEVIINGNDLKKALEQTAFAMSKEDMRPAMTGTLVE
                     FAKEGLRFVATDGHRLVKFINKSFSSEVQEQYIIPERAISVLLKQLGDADVKIYLSKT
                     YASFIINDLEFISRLIGEKYPSYSSVIPLENENFLTVNRSELLSAIKRMMLFSTSNSK
                     QVKFSIGENSLEVSAEDVDHGSDAKENIQCEYKGDPMDIGFNTTYVNDILSHLVGDQI
                     VFKLHSPTKASIIEPVKKEENEDIMMLLMPVRLNN"
     gene            7059..8168
                     /gene="recF"
                     /locus_tag="VF870_02800"
     CDS             7059..8168
                     /gene="recF"
                     /locus_tag="VF870_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558877.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX9251138.1"
                     /translation="MILSRLSLSNFRIHKNINLTFSDTLNYIVGGNGQGKTSVLEAAY
                     YLCTTKSFNAKDIEAVSFGENDFELNGFFNDITEDKIRIIFSLPENRKHYFQNDKLIS
                     RSSQIIGKFPVVILTPADHAITQGSPAERRKLVDSVISQASSTYLEILLDYNKTLKQR
                     SVVLNRLRENFTKAAVAELEAWTEKLIENGLELILHRKTFINNFNKYVSDSYKKIIGE
                     KENPTIEYSFLDDSQDDEIRNTFQSLIKEKRDEEIRRSVNLVGPHRDDFIFKINDVNL
                     RTYGSQGQHKTFQTALRFAEFFYLKDTSGKTPIFLLDDVFGELDAYRAGKISEYLKEV
                     GQAFITLTDFADFSFLQKDENDLIIKLENGNIAYA"
     gene            8161..8451
                     /locus_tag="VF870_02805"
     CDS             8161..8451
                     /locus_tag="VF870_02805"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX9251139.1"
                     /translation="MPDGFKSIKDVFNNDPALKKIRTVINENDVVNDFEKIFPEFKKV
                     VQAKKTHNTVITLKVENAAWRNELKFKEKEIIEKINSYYGEERITKIIFSAR"
     gene            8476..>8906
                     /locus_tag="VF870_02810"
     CDS             8476..>8906
                     /locus_tag="VF870_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9251140.1"
                     /translation="MAKAQNNNSNDYSAKNISVLKGLEAVRKRPAMYIGDVSSRGLHH
                     LINEVVDNCIDEALGGYNDRVIVIIKKDGSVSVEDRGRGIPVDMHPVEKRSALEVVMT
                     VLHAGGKFDKDSYKVSGGLHGVGVSVVNALSEKMTAEVRRD"
BASE COUNT         3023 a         1372 c         1495 g         3016 t
ORIGIN      
        1 gatgtgggca gaattggatt taggccatca agccgcaatg aaaaaatagc tctgtcttct
       61 gtaacttttt gatcaataaa aagatctctt ctgtctgatt gggtgttcag ggtaagtaaa
      121 tcattccagt ctgtttggtg agcaattgaa tcaagctgat caacagtata gccagcagca
      181 tacaatccgc caactatgct gcccatgctt gttccaacaa ttaaatccgg ctttattccc
      241 tcttcttcta aagcttttaa aacacctatt tgtgaaagcc ctcttgcacc tcctccgctt
      301 aaagcgaatg cgatttctgg tttgtgtgct ggaacaaaat ctgttagtcc aaaaggaagt
      361 tttttttctt ttagatctaa cgacaaaata tattttgtct ggctaaaaat atttgagata
      421 aaaaaaacga aaactattaa tattattttt atctgttttc tcatctaatt attttattct
      481 caaataacag aagttaaccg agcattattt ctttttcttc tgatgacttt ttgattgctc
      541 ttctgcggct tccattaatt tagacataaa tccttttttc ttatttggat ttgccaccgg
      601 aacaagttca accgtaccgc ctttatgatt gatataatat tgctgggcaa tggacaatac
      661 gttaaacata aagtagtaaa gatttaatcc tgaaggaaaa ctcataaaaa gaagcgtcag
      721 cataataggc atcatataaa tcaatgcctg ttgtttggga tcttttactg tcatcttttg
      781 ctgcacaaag gttgtaattc ccatcaacaa agctaacccg cttatttcct ggattccaat
      841 taagggcagc tttgtaccaa gatgaaatat tacatccggt tgagaaagat ctgtaatcca
      901 tccaacaaat ggttgttggc gcaagtctat tgccgattgg aacattcccc acagggcaac
      961 aaaaattggc atctggagta acattggcaa acatccgcca gctgggttta taccgtaggt
     1021 cgaataaagt ttcattgttt ctttattcat cttctgcgga tcatctttaa acttttcttt
     1081 cagttcggca atcatcggtt gtaaggcttg catctttttc attgattgat agctggattt
     1141 tgtaagtgga tatacaacta tctttataat tatggaaaag attatgagta caacaccata
     1201 gtttggaatg aatgaattga taaactgaaa caacggcagc aaaatatatt ctgcaatagg
     1261 cctaactata aattttaaac caaagaaact tccgaaatcc acaatcttaa ctaagtttct
     1321 gccgtatgct ttaagttctt tataatcaac cggtccaata aataatgtaa acgattgtga
     1381 ttcgattttt ttattttgaa aaggaagcgt cagtcttgca ttatacgttt catgtattcc
     1441 gtcttttcca actttttcat gtgttccttc aatgtaagcg ccttcaactt tttgaggatc
     1501 gtccggaatt attactgctg caaaatattt attccttacc gtaagccaat caactcttcc
     1561 gcggaattct ttaacttctt tttctccaac agaagatgca tttactataa cttgttcatc
     1621 gccgtaataa acacttgcat ttgagtaatt agcttcatca acagaatttc tttcaatgga
     1681 tcgaattcca ttactccaaa tcaagtcata agagttgttg cttataagat tattaaatcc
     1741 gtcaaaagat attttacttt ttatttcata cttgtcgcca aaaaaagtat atgtttttat
     1801 aagagttttg cccggttcta attcaaaaga ataattaatt gtaagcgagt cgtttttatc
     1861 aattttgtat ttgccggcag gtttgtctga attgaatgta atgtcctttg tatttattcc
     1921 tttaccatca accgagacaa acgaaacatc ataagtgccg ccttttttat agtctataag
     1981 ttgaacatag tcagaaaaat ttgtttcccc ttttttgttg gccgagtacc aatttctaaa
     2041 ttttgttaaa aatatctttc taaagtttgc gcctttattt gaaagctcaa acctggctaa
     2101 ttgtgtttca ataacaagat actcttccgg aattgttgat tcaaaaatgc ttacgttttc
     2161 tgttgtcgtt tgttccggag ttgtttttga ttcagctatg ctgtctttgg taacagcttt
     2221 aacagagtct gttgtttgtg tggtttcgct tttttgttta ggctgcttat taggatcggg
     2281 tgcgcttatg taaagccaca aaaccaaaat ggcgccaatt aaaataaatg caattgttgt
     2341 ttgcttatcc atcaataaaa actctaatat taaataacgg ttgaagaaat cttaattaat
     2401 tccggcgtgc taagaagtgt agtttttttt tgaagttttc ttttcaaaat aatctattaa
     2461 atcttttgta ttattttact catcagttca acaacaccag gagaaatatc acgcaaatat
     2521 atttttttat tacttgcttc cgataatctg tttggagaaa aaactaaaag taattgaata
     2581 ttcttttcta aagctttttg cagcaataat tttttattaa gtctatatgc ttcttttatt
     2641 attctcttta ccctgtttct ccaaacggcc ttgcctgctt ttttggatac tgcagcagct
     2701 atttttactc ccgccacgtt actatcaaca aacaaaaaat gcaattttat taaacgatct
     2761 gagctgatca gtatattatc agaacaataa atcttttcaa aatctttttt cttttttacc
     2821 ctctccttgc gggttaaaga aaaatttttc aaaattatta tttctcatca ctaacggtaa
     2881 gtttttttct gcctttcgct cttcggctgg ctaaaactct tctaccgttt tttgttttca
     2941 ttctttctct gaagccatgt ttattttttc gttttctgtt acttggctga aatgttcttt
     3001 tcattttgtt attcctgttt tacataaagt ttgcaaaaat atagatagat tcaaatttat
     3061 ccaacaaatt taagcaacca cttgctttaa atctcaaaga aatgttcgat aatactaagt
     3121 aaagatttgt attagtatac gttaccaaag cactatcatt taataaacgc tgtaattctg
     3181 ttaattttaa tacttttcac attaaatgtg aataattatg gtctatatta ctcttctaaa
     3241 ataaaattga aaaaaaatat attagaataa acttgctgat atgatgtttt gtctctattt
     3301 tttactaagc ttggtgcagt tctttttatg tttataacct gtggataact taaacttgtg
     3361 aaaacgtttc acattgtaaa gggatattaa attcaggtaa aaattaccgc cagctatttg
     3421 ataacatgaa aataatcctt ataacttatt gaaaaatcta tagttaaccg ctgttcaata
     3481 atgttaacaa cttgtgaatt tgttttttgt gcttaactaa atcaatataa tattttctat
     3541 aatgatcgct ttttacgctt ataactattg tggataacct aaaatttatc aacctggaaa
     3601 cgagcacttc tgccgtaaat acagatgcta atttgatctg gaaaaactgt ttaacccgca
     3661 ttaaagaaaa cgttacttta atgacttaca atacctggtt tttaccaatt agacctgtta
     3721 gttttagtga taacaactta aaagtccagt taccaaatca atttttctgg gaatggatag
     3781 acgaacattt cagcactatt gttaaaaaga caatatttga ggttcttgga gaaaatggga
     3841 aactatccta tataatctcc gatgagcagg aacaaaaaga agaatttgta aaacaaagct
     3901 ctttgattaa taccaatgta gagaaaacga tagaaaccaa acgcccggaa catgaaacaa
     3961 atttaaatcc ccgctacaaa tttgaaaatt ttataaaagg cgaaggcaat cagcttgcaa
     4021 gagctgccgc tggcgctatt agcgataatc ctggtggaac ttctttcaat ccttttttta
     4081 tttatggtgg tgtaggatta ggaaaaacac atcttataca atcgattgga aatagtatca
     4141 ttgaaaaatt tcctgagaaa aaagtaattt atctttcttc tgattctttt accgttgagt
     4201 tcgtagaagc tattcaatca aataaggtta atgatttttc cggcttttat agaaacatgg
     4261 atgttttaat aattgatgat atccagtttc ttatgggtaa agaaaaaacc caagatttat
     4321 ttttccatat ttttaatacc cttcaccaat caagaaaaca aattattctt tcaagcgata
     4381 agcctccaaa agatttgaaa ggtttggatg aaagattgat ttctcgtttt caatggggat
     4441 tatctgccga tatacagccg ccggaacttg aaatgagaat tgctattctt aaaaacaaag
     4501 cagaagaata tggaatgctt gtttcaaccg agatattgga gtttattgct ggcagtataa
     4561 catctaacat tcgtgaactt gaaggctgct taataaaatt acttgcaaat gcttctttaa
     4621 actctaaaga aattactctt gaactggcaa agagaacggt taaagaaatt gcaacagata
     4681 gaaaaataaa tattaccatt gatgatataa gtaaagtggt ttgtcactat ttaaacattc
     4741 ctgaaaataa gattagagaa aagacccgaa agaaagaaat tgttcttgca agacaattag
     4801 cgatgtactt ttctaaagaa cttactaaat cttctttaaa gacaataggg ctgcactttg
     4861 gcggcagaga tcattcaacc gtaatacatg cttgcggcag cattgaacaa atgagaaata
     4921 atgatctttc tatgaaagag ctgattgata gtattagaaa caagattgac cttaattatt
     4981 cctgaaattt ttaccagagt gttttatggc tcctagtgga gccttcgttt ttttgattta
     5041 ttgtgtgttt tagtgatgtt aataaggagt gaatgaatgg tatataaatt gttaacaaat
     5101 actgatttat atagactctg ttattaacct tatttttaac gagcctataa ttaactttaa
     5161 atgttggttc aatagtttag ttataatata aaaacagata ataaaaaggt tattaacata
     5221 ttaacacagt tattataatt attaatttaa aactatatat ctattaatta ttaatattgt
     5281 gttaattgat ttctaacttg aattagttaa attttgagaa acaaaaatat ctcattattt
     5341 aaaaattaaa tgagagagga aaaaatgaaa aaacaactat tacttctttt tactttggct
     5401 gtagtttctg taattgtttt tacaagttgc gaaccattta ttgaaaataa aattacggtg
     5461 agaaacgaag catctggcga tgtaacactt attgttgctg gaaaaactta tgacgttgct
     5521 gcaaattcaa gtttagtact accagatttc aaaaaaggaa cctttgcata ttcaacagtc
     5581 tatagtgttc ctttgggagt tacagatgca agtgcagaag gagatgtttc aggaaatttt
     5641 gttttaaatg ccggcacaga aattcttctt caatatacaa gtgtaacagt tgactcttca
     5701 tatacaattt ttggtattct aacatcttca gacgatgtga acagagttga tcctatcata
     5761 gattctggca attaacaata aatattttcg attaaaacag gagttgaata actttaattt
     5821 aactcctgtt tttgttatta accacttctt acaaccattc ccggtattaa aatttaatct
     5881 ctgaattttt attgaaatat gcaccttttt tagtattttt aggcgcttca aaaggagata
     5941 tttatatatg gaatttaagt taaacagcaa gattttagaa aaattattag ccaaaattat
     6001 tcccgctgtt ccatcccgta caccaatgcc tatattggaa aactttttat ttgaaattaa
     6061 agacggctca cttactgtaa gtgcaacgga tcttgaaata gcactgcgat catcattaaa
     6121 tgttgcagca gaaaaaaata ttaaaatggt agttcctgcc agattactat acgatattgt
     6181 tagatctctc gaagatacac aagtaagctt tttagtggat agcaagggaa aaattaaaat
     6241 cactacggat aaaggcgtgt atagtttaag ctactctcca tctgaagatt ttccggaaat
     6301 tgcaaccgta tcaaaagaaa aagaagtaat tattaatggt aatgatttaa agaaagctct
     6361 tgaacaaaca gcttttgcta tgagtaaaga agatatgcgc cctgcaatga caggaaccct
     6421 ggtagagttt gcaaaagaag gtttaagatt tgttgccaca gacggacaca ggcttgttaa
     6481 atttattaat aaatcttttt catcagaagt acaggaacaa tatataattc ctgaaagagc
     6541 catttctgtt ttgttaaaac aactaggaga tgccgatgtt aaaatttatt taagcaaaac
     6601 ttatgcatca tttataatta atgatcttga atttatcagc cgtttgatag gagaaaaata
     6661 tccatcctac agcagcgtta ttcctttgga gaatgaaaac tttctaacag ttaatagatc
     6721 agagttgcta tcggcaataa aaagaatgat gcttttctca acttcaaatt ctaaacaggt
     6781 taaattttca attggcgaaa attctttaga agtatcggca gaagatgttg atcatggttc
     6841 agatgcaaaa gaaaatattc agtgtgaata taaaggcgat cctatggata ttggcttcaa
     6901 cacaacatat gttaatgata ttttatcaca tcttgtggga gatcaaattg tctttaaact
     6961 tcattctcca acaaaagcaa gcattataga gcctgttaag aaagaagaga atgaagatat
     7021 tatgatgtta ctaatgccgg tgcgattaaa taattaaaat gatcctgtcg cgtttatctc
     7081 taagcaattt cagaattcat aagaatatca accttacctt ttctgataca ttaaattata
     7141 ttgtgggagg taatggacag ggaaaaacct cggtgctgga ggcggcatat tatctttgta
     7201 caacaaaaag ttttaacgct aaagatattg aagccgttag ctttggtgag aatgattttg
     7261 agttgaatgg gttttttaat gatataactg aagataagat tagaataatt ttttctttac
     7321 cagaaaaccg caagcattat tttcaaaacg ataaattgat ttcaagaagt tcacaaataa
     7381 taggaaagtt tcccgttgta attttaactc ccgcggatca tgcaattact caggggtctc
     7441 cagcggaaag aagaaaactt gttgattctg ttatatctca agccagcagc acatacttgg
     7501 aaattttgtt ggactataat aaaacgctaa aacaacgttc agtagtttta aatagattga
     7561 gggaaaattt tacaaaagca gctgttgcag aattagaagc atggacagaa aaattaattg
     7621 aaaacggatt agaattaatt ctacatagga aaacgttcat aaataatttt aacaaatatg
     7681 tttctgattc ttataaaaaa attattggtg agaaagaaaa tccaactata gaatatagtt
     7741 ttcttgatga tagccaagat gacgaaataa ggaacacgtt tcaatcgctt attaaagaaa
     7801 aacgagacga agaaataagg cgcagtgtta atcttgttgg tccgcataga gatgatttta
     7861 tttttaaaat taatgatgtg aatctaagaa catatggttc tcaggggcag cacaaaacat
     7921 ttcagacggc actaaggttt gcagaatttt tttacctgaa agatacaagc ggcaaaacac
     7981 caatttttct tttggatgat gtgtttggtg aacttgatgc ttatcgcgca ggaaaaataa
     8041 gcgagtatct taaagaagtt ggacaagcat ttataacact aacggatttt gcggatttct
     8101 cttttttaca gaaagatgag aatgatttga taattaaatt agaaaatgga aacatagctt
     8161 atgcctgatg gatttaaaag cataaaagat gtttttaata atgatccggc attaaagaag
     8221 ataagaacag ttattaatga aaatgatgtt gtaaatgatt ttgaaaagat ttttcctgag
     8281 tttaaaaaag ttgtgcaagc aaaaaaaaca cacaacacgg taattacgct taaagtagaa
     8341 aacgccgcat ggcgtaatga gttaaaattt aaagaaaaag aaataataga aaaaataaat
     8401 tcatattacg gcgaagaacg aattactaaa attatttttt cggcccgata aagaattaaa
     8461 ggaatataag cagatatggc taaagcacag aacaacaatt caaacgatta cagtgcaaaa
     8521 aatattagtg tacttaaagg actggaagcg gttcgtaagc gtccggcaat gtatataggc
     8581 gacgtaagtt ctcgcggact tcatcaccta ataaatgaag ttgtggataa ttgtattgat
     8641 gaagcattgg gaggatataa tgaccgcgtt atagtaatca ttaaaaaaga tggaagcgtt
     8701 tcagttgaag atcgtggaag aggaattcct gttgatatgc atcccgttga aaaacgctct
     8761 gcattagaag ttgttatgac agttcttcac gcgggaggaa agtttgataa ggattcatat
     8821 aaggtttcgg gaggattgca tggtgtgggt gtttcggttg taaatgcttt atcagaaaaa
     8881 atgactgcag aagtacgaag agacaa
//
LOCUS       DASWBY010000150         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_42924, whole genome shotgun sequence.
ACCESSION   DASWBY010000150 DASWBY010000000
VERSION     DASWBY010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42924"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VF870_02815"
     CDS             <1..558
                     /locus_tag="VF870_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9251141.1"
                     /translation="ILYSKGEVAVKGIRNFAVRLAGERGDAIVTLAGQAVKAVTMGVV
                     ITALIQSVLGGVALWICGVPRPGLLTAIMFILGLTQIGGLPVLLPAVIWKYTTGDPVW
                     GTVLLVLAILVGASDNFIRPYLIKRGADLPILLIFAGVIGGLIAFGIVGLFIGPVILA
                     VSYTLLKAWINEHKGDSVAETNTGS"
     gene            697..1860
                     /locus_tag="VF870_02820"
     CDS             697..1860
                     /locus_tag="VF870_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9251142.1"
                     /translation="MRKRLNVFTFLLLVAMVTVLTTACGEEEKQAGAPTEVQVVKAVK
                     MNVPITGEWVGQTLGAVDIEIRARVEGWLQSINFKEGSEVKKGALLYTIDATELREKV
                     AEAEGKVASARTLLVQAEEDVKRYTPLAEVGAVSKRDLEIAISRYEAQKGELDAALAQ
                     LNVAKTNLSYATVTAPITGLIGISAARVGDFVGRPPNAVILNTISRVDSIHVRFSISE
                     SEYLNLVRQIEREGKTNVKAKEIQMILADGSAYPNLGVISFAQRQIDPATGTLQFEAS
                     FPNKERMLRPGQFAKVRIVVDERKDAVVVPSRAIFEIQGQKNVYVVDENNKVIMRVVT
                     TGPEYNNNVVIETGVKPNETVIYEGLLKVRPDMIVTPTEAKLSEGQKKTSGEK"
BASE COUNT          587 a          294 c          430 g          572 t
ORIGIN      
        1 atactttact ccaaagggga ggttgctgta aaaggaatta gaaattttgc agtgcgtcta
       61 gcaggtgaaa gaggtgatgc aatagttaca ctggcaggtc aggcagttaa agcagttacg
      121 atgggtgttg taataactgc acttattcag tcggtattgg gtggggttgc gctctggatt
      181 tgcggagttc caagacccgg attgctgaca gctattatgt ttattcttgg actaactcaa
      241 attggtggac tgccagtgct acttccagcc gttatctgga aatatactac cggtgatcct
      301 gtttggggaa ctgtgctatt agttctagct attttagttg gtgcttctga taatttcatt
      361 cgaccgtatc ttatcaaaag aggtgctgat cttccaatac ttctaatttt tgctggtgtc
      421 atcggtggat taattgcttt tggaatagta ggattattta tcggtcctgt aatccttgca
      481 gtaagttata cattattaaa agcatggata aacgaacata aaggagattc agttgctgaa
      541 accaatactg gcagttagca aatttatttg agtaagattt tttttaatac tgatgagcta
      601 aattaaagtt ttttaactaa tttttaacat aatttaattt tttacaaaat gaatattcag
      661 tacttatttt ttataggagt aattcaaatg atgaaaatga gaaaacggct aaatgttttt
      721 acttttctgt tattggttgc aatggtaaca gttctaacta cggcttgtgg tgaagaagaa
      781 aaacaagcag gtgcaccaac cgaagtccag gttgtaaaag ctgttaagat gaatgtacca
      841 ataacagggg agtgggttgg acagacactt ggtgctgttg atattgaaat cagggcaaga
      901 gttgaaggct ggctgcagag tattaatttc aaagaaggaa gcgaagtaaa aaagggagct
      961 ttactctaca caattgatgc aactgaattg cgtgaaaaag ttgctgaagc ggaaggtaaa
     1021 gtagcttctg cccgaacttt attggtgcag gcagaagaag atgttaaacg ttacactcct
     1081 ctcgcagaag ttggcgcagt aagcaaacgt gatcttgaaa tcgccatttc cagatatgaa
     1141 gcacaaaaag gtgaacttga tgcagcactt gctcaattaa atgtagctaa gactaatctt
     1201 agttatgcaa cagtaaccgc tcctatcacc ggattaattg gtatttctgc tgcacgagtt
     1261 ggagatttcg tgggtcgtcc gccaaatgcc gttatattaa atacaatttc ccgggtcgat
     1321 tcgattcatg ttcgcttttc aatttcagag agcgaatatc ttaatcttgt acgtcagatt
     1381 gagagggaag gaaagaccaa cgtaaaggca aaagaaatcc agatgattct ggctgatggt
     1441 tctgcttatc ctaaccttgg agtaataagt tttgctcaaa gacagattga tcctgctaca
     1501 ggcacattac agtttgaagc ctctttcccg aataaagaaa gaatgttaag acccggtcag
     1561 tttgccaaag tacgcatagt ggttgatgaa agaaaagatg ctgttgttgt tccttcgcga
     1621 gcaatatttg aaattcaggg gcaaaaaaat gtttatgttg ttgatgaaaa taataaagtc
     1681 ataatgaggg ttgtaacaac tggtcctgag tataataaca acgttgtaat tgagactggc
     1741 gttaaaccta atgaaactgt tatttacgaa gggctattaa aagtgagacc tgatatgatt
     1801 gttaccccaa ctgaagcaaa gttatctgag gggcagaaaa aaacttcagg ggagaaataa
     1861 tatatggcta acttttttat acg
//
LOCUS       DASWBY010000151         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_683188, whole genome shotgun sequence.
ACCESSION   DASWBY010000151 DASWBY010000000
VERSION     DASWBY010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683188"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VF870_02825"
     CDS             <1..538
                     /locus_tag="VF870_02825"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX9251143.1"
                     /translation="AESGVHRYNTRKKIDDQSAWTYYKNNGRNFNAEGISVSVSELAF
                     VAKEIWFGTDEFITNDQPDYNVGGIYVWNRKLKWDIISKANGLGGNGVYCLARTGNYI
                     WAGVYEFKKNDKEEYGKGLYLINRLTKKVEPVDINQLNLSTSNIHTLYFDGINLWIGT
                     SDGLIRLKVGSELAHIKK"
     gene            750..1379
                     /locus_tag="VF870_02830"
     CDS             750..1379
                     /locus_tag="VF870_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251144.1"
                     /translation="MKYFLFALTILLTLNVNSTFSQIQAQSSSNINNHDGFFLRMLLG
                     FGQTDLTEEMDYGGDLKMSGVGTEFRVQIGGCVSENLILFGDLGGVVVMSPNIEWAGN
                     STSSDNTSLTISEFGGGLTYYFMPENIYVSFSAVLPVATIEFNTTKGESKMGFGFFLS
                     AGKEWWIAEQWGLGAALYGSYGSMKDKGEFADNAISTYSFGVAFSATLN"
BASE COUNT          564 a          261 c          330 g          556 t
ORIGIN      
        1 agctgagtcc ggtgttcatc gatacaatac aagaaaaaaa attgatgacc agtctgcctg
       61 gacctactat aaaaacaacg gaagaaattt taatgcagag ggcatctctg tatcggtttc
      121 tgaacttgca tttgttgcta aagaaatatg gttcggcact gatgagttta taacaaatga
      181 tcagcccgac tataatgtgg gagggattta tgtctggaat agaaaactta aatgggatat
      241 aatctccaaa gctaatggac ttggcggtaa tggcgtttac tgtcttgcac gaacgggaaa
      301 ttatatctgg gcaggggtat atgaatttaa aaaaaatgat aaagaagaat acggcaaagg
      361 attatatcta attaatcgct taacaaaaaa agttgagcct gttgatatta atcaattaaa
      421 tttatcaacc tcaaacatcc atacgcttta ctttgatggg attaatcttt ggatcggaac
      481 atctgatgga ttaataagat taaaagttgg cagtgaactt gcacacataa agaagtaata
      541 tttcacaaag attattaatt ggcaattatc aacttttaat tttttgacaa tgcccaaaat
      601 tagtttagat ttattactaa caaaataaaa ctactccgag agggctgaac attagggcca
      661 ttcttgctgt taagtcctga gacaaactcc aataactctc ccgaagcaga agcgttaaac
      721 aagtatctta aatttaccgg aggtgtaaga tgaaatattt tcttttcgct ttaactattc
      781 ttttaaccct caatgtcaat tcaacattct cacagattca agctcaatca tcctcaaaca
      841 ttaataacca cgatggattc ttcttaagaa tgctattggg atttgggcaa accgatctaa
      901 ccgaagaaat ggattatgga ggtgatctaa aaatgtcggg tgtcggtaca gaattcaggg
      961 ttcaaattgg tggttgcgtt tctgaaaatc ttattttgtt tggagattta ggtggtgtgg
     1021 ttgttatgtc acctaatatt gaatgggctg gcaattctac tagtagtgat aacacaagtc
     1081 tcacaatttc tgaatttggc ggtggtttaa catattactt tatgccggaa aatatttatg
     1141 tatcatttag tgctgtatta ccagtagcta caattgagtt taacactacc aaaggtgaat
     1201 caaaaatggg atttggattt ttcctatccg ctggaaaaga atggtggata gcagaacaat
     1261 ggggtcttgg tgccgcattg tatgggtcat atggcagtat gaaagataaa ggagagttcg
     1321 cagataatgc tatttcaaca tattcgtttg gagtagcttt ttcggctaca ctcaattagc
     1381 taattgaatt caaactattg actattcatt gagaatgaat attgaaagga ttcatttatt
     1441 taagcactgt caatttatca cttaacatta gaaaggcgat tgttaaatcg cctttttagt
     1501 actttttacc cacatttttt atagttattt ttaagccaaa tttattaact aatctggctt
     1561 ttcaatgatt aaaatttctg aaaaaagatt aaagcagtta aaagcaaatt ttaatggtaa
     1621 acgtattgcc attattggcg atatgatgct tgatatttat ttctggggtg acgtaaaaag
     1681 aatttctcca gaagccccgg ttccggtttt a
//
LOCUS       DASWBY010000152         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_306488, whole genome shotgun sequence.
ACCESSION   DASWBY010000152 DASWBY010000000
VERSION     DASWBY010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306488"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /gene="sufB"
                     /locus_tag="VF870_02835"
     CDS             <1..1265
                     /gene="sufB"
                     /locus_tag="VF870_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX9251145.1"
                     /translation="WLTMEEPKWPNVTYPPVDYQDISYYSAPKRKPALKSLDEVDPEL
                     LDTYKKLGISLDEQKMLAGVAVDAVFDSVSVATTFKSKLNEMGIIFCSFSEAIREHPD
                     LVKKYLGSVVPYTDNYYATLNSAVFSDGSFVYIPKGVRCPMELSTYFRINAANTGQFE
                     RTLIIADDNAYVSYLEGCTAPMRDENQLHAAVVELVALENSQIKYSTVQNWYPGDKDG
                     KGGIYNFVTKRGACRGTNSKISWTQVETGSAITWKYPSCILQGDNSIGEFYSVAVTNN
                     MQQADTGTKMIHLGKNTRSTIVSKGISAGRSNNSYRGLVKIGKKAEGARNFSQCDSLL
                     LGDKCGAHTFPYLEINNPTAQVEHEETTSKIGEDQIFYLNQRGISTEDAVSLIVNGYC
                     KEVFKELPMEFAVEAQKLLSISLEGSVG"
     gene            1364..2128
                     /gene="sufC"
                     /locus_tag="VF870_02840"
     CDS             1364..2128
                     /gene="sufC"
                     /locus_tag="VF870_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX9251146.1"
                     /translation="MLLEIKNLHANVEGTEILKGINLKVNAGEVHAIMGPNGSGKSTL
                     ASVLAGKEEYEVTAGEVLYNEKNLLELSPEDRAREGVFLAFQYPVEIPGVSNTNLLKT
                     AVNEIRKYRGEEELDSMDFLSLLKEKSKLVELDSKFLSRAVNEGFSGGEKKRNEIFQM
                     AVLNPTLAVLDETDSGLDIDALRIVANGVNKLKRKDNATIVVTHYQRLLDYIIPDFVH
                     VLYKGKIVKSGGKELALELEEKGYDWIKSDKAEALV"
     gene            2240..3556
                     /gene="sufD"
                     /locus_tag="VF870_02845"
     CDS             2240..3556
                     /gene="sufD"
                     /locus_tag="VF870_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX9251147.1"
                     /translation="MENKTDIKKYFINQFDEFEKSLNGEKSSDFHKIRKDAINKIAGI
                     AFPTQKDEEWKYTNISSLQKHNFSLAAIKANVSSETISKFLFDKMEHSLLVFVNGNFS
                     PELSKLIDIPKRVVIGSLAEALKEDNPIVKKHLGKYAENENYFFTTLSSAFTKDGAFV
                     YVPDGKVVEDPIHIIFITKSGSEKILTQPRNLFVAGKNSQVTIIEHYVSDEEGVYFTN
                     SVTEIVTDENAIVDHIKLQEESNKAFHIARMEVDQERSSNFSSHLISHGAEISRNDFN
                     ARFNDEGSECMLNGLFMIGNEQIFDAHTMIDHAKPYCNSHEHYKGILQDKSKGVFNGK
                     VMVRQDAQKTNAFQENNTILLSDDAVMNTKPQLEIFADDVKCSHGATIGKLNDEAKFY
                     LKSRGIGEDSATAILIHAFASDVITSIKIPALRDYLEEIISKRFNA"
BASE COUNT         1297 a          558 c          727 g         1065 t
ORIGIN      
        1 attggttaac gatggaagaa ccgaaatggc cgaacgtaac atatccgcct gtggattacc
       61 aggatatatc atactactca gcacccaaaa gaaaacccgc attaaaaagt ttagatgaag
      121 ttgatcctga attgcttgat acttacaaga aactgggaat ttctctagat gagcaaaaaa
      181 tgcttgccgg agtcgcggtt gatgcagtgt ttgattcagt ttctgttgct acgactttta
      241 aaagcaagtt aaatgaaatg ggaattattt tctgttcatt ctctgaagcg attcgtgagc
      301 atccggatct tgtaaaaaaa tatttaggaa gtgtagttcc ttacacagat aattattacg
      361 caacattaaa ctctgctgtt ttcagcgatg gttcgtttgt gtacattcca aaaggtgtaa
      421 gatgcccgat ggaactttcc acatacttta gaattaacgc cgctaacaca ggacagtttg
      481 aaagaacatt aattattgca gatgataatg cctatgtaag ttatcttgag ggctgcaccg
      541 caccaatgag agatgaaaat caattgcacg cggcagtagt tgaattggtt gctcttgaga
      601 attcacagat aaaatattcc actgttcaaa attggtatcc cggtgataaa gacggcaaag
      661 gcggaattta taatttcgtt acaaagcgtg gtgcctgtcg aggtactaac tcaaagataa
      721 gttggacaca ggttgaaacc ggatctgcaa tcacctggaa atatccaagc tgtattcttc
      781 agggagataa ttcaattgga gaattttatt ctgttgctgt aactaataat atgcagcagg
      841 cagatacagg aacaaagatg atccatcttg gaaaaaacac cagaagtaca attgtatcaa
      901 aaggtatttc tgctggaaga agtaataaca gctatcgtgg attagttaag attggaaaga
      961 aagctgaggg cgcgcgtaat ttttcacaat gtgattctct tttacttgga gataaatgcg
     1021 gcgcacatac tttcccatat ttagaaatta ataatccaac tgcacaagtt gagcacgaag
     1081 aaacaacatc taaaattggt gaggatcaga ttttctatct taatcaaaga ggaatatcta
     1141 ccgaagatgc agttagcttg atcgtaaatg gatattgtaa agaagttttt aaagaacttc
     1201 ctatggagtt tgctgttgag gcgcaaaaat tattgagcat aagtttagaa ggaagtgttg
     1261 gataaatgct cttcatcccg gacttgtttc gggatctctg gtaaaaaata agaagctgaa
     1321 acgagttcgg cttgacaagt ttgaaaataa ataaggaaat aaaatgttat tagaaataaa
     1381 aaatcttcac gcaaatgttg aaggaactga aatcttaaaa ggtataaact taaaagttaa
     1441 tgctggtgaa gttcacgcaa ttatgggtcc caacggatct ggcaaatcaa ctttggcatc
     1501 tgtacttgca ggtaaagaag aatatgaagt tactgccggc gaagttttat acaatgaaaa
     1561 aaatctttta gagctttctc ctgaagatcg tgcaagagaa ggagtttttt tagcgtttca
     1621 gtatcccgtt gaaattcccg gtgtaagcaa cacaaatctt ttaaaaacgg cagttaacga
     1681 aataagaaaa tatcgcggtg aagaagaact agattcaatg gatttccttt cgctgttaaa
     1741 agaaaaaagt aaacttgttg aacttgattc aaaattttta agtcgtgccg ttaacgaagg
     1801 gttttccggc ggtgagaaaa aacgtaatga aatttttcaa atggctgtac taaatccaac
     1861 acttgctgtg cttgacgaaa ctgattccgg tcttgatata gatgcactaa gaatagttgc
     1921 aaatggtgtg aacaaattaa agagaaaaga caatgcaaca atcgttgtaa cgcattatca
     1981 aagattactt gattatataa tccctgattt tgttcacgta ctttacaaag gtaagattgt
     2041 aaaatctggc ggaaaagaat tggcccttga gcttgaagag aaaggttatg attggattaa
     2101 aagtgataaa gctgaggcat tggtttagtt aataattcag tgtcacactg agcgaagtcg
     2161 aagtgtgaca gaaattaaat gtcagccttc gactgcgctc agtctgacaa atcaataatt
     2221 gaattagaat ttagagtaaa tggaaaataa aacagacata aaaaaatact tcatcaacca
     2281 gtttgatgaa tttgagaaat cattaaatgg agagaaatct tctgacttcc acaaaataag
     2341 aaaagatgca ataaacaaaa ttgctggtat cgcttttcca actcaaaaag atgaggaatg
     2401 gaagtacaca aatatttcat ctttgcaaaa gcacaacttc tccctcgctg ctattaaagc
     2461 aaatgtttca tcagaaacaa ttagcaagtt tttatttgat aagatggaac atagtctttt
     2521 ggtttttgta aatggaaatt tttctcctga gttatcaaaa ttaattgata tcccaaaaag
     2581 agttgtgatt ggaagtcttg cagaggcttt gaaagaagat aatccaattg ttaaaaaaca
     2641 tttaggcaag tatgctgaga atgagaatta ttttttcaca accttaagtt ctgcttttac
     2701 taaagacggt gcatttgttt atgtacctga tggaaaagtg gttgaagacc cgattcacat
     2761 aatatttata acaaagagtg ggagtgaaaa gattttaact cagccaagaa atctttttgt
     2821 tgccggtaaa aattctcagg ttactatcat cgaacattac gtttcggatg aagaaggtgt
     2881 ttattttaca aattcggtta cagaaattgt tacagacgaa aatgcaattg tagatcacat
     2941 aaaattgcaa gaagaaagta acaaagcatt ccacatagca cgaatggaag ttgaccagga
     3001 acgaagcagt aatttttcat cgcatttaat ttctcacgga gcagaaatct cccgcaatga
     3061 ttttaatgca agatttaatg atgaaggcag tgagtgtatg cttaacggtt tatttatgat
     3121 cggtaatgaa caaatttttg atgctcatac tatgatcgat catgcaaaac cgtattgtaa
     3181 cagtcacgaa cattataaag gaattttaca ggataaatcc aaaggcgttt ttaatggaaa
     3241 agtgatggtt cgccaggatg cacaaaaaac aaatgcgttc caggaaaaca acacaattct
     3301 actttctgat gatgctgtaa tgaacacaaa accccaatta gaaatttttg ctgacgatgt
     3361 taagtgctcg catggtgcaa ccattggtaa gttaaatgat gaagctaaat tttatttaaa
     3421 atcgcgcggt ataggtgaag actcagcaac agcaatttta attcacgctt ttgcaagtga
     3481 tgttataact tcgattaaaa ttcctgcttt aagagattat cttgaagaaa ttataagtaa
     3541 aagatttaac gcatagaaag taaaaatgca tactaaaaat gcagcaatca ttgagagtaa
     3601 atcaaatctt tatgatgtgc agaaaattag aaacgatttt ccaattt
//
LOCUS       DASWBY010000153         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_8062, whole genome shotgun sequence.
ACCESSION   DASWBY010000153 DASWBY010000000
VERSION     DASWBY010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8062"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..>1831)
                     /locus_tag="VF870_02850"
     CDS             complement(41..>1831)
                     /locus_tag="VF870_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9251148.1"
                     /translation="VKGTNNDGIWNEEGIALKLIITPPWWSRWWAYLLYLMFILSILY
                     LIRRYEMNRLKLKNQLKLEKVETDTLRHLDNLKSHFFANISHEFRTPLTLILGQIESV
                     LGSNIDVREKGKLQVANRNARRLLTLINQLLDLSKLEAGSMELKAEQYNIVSFLKNLL
                     FSFESLAVSKKIKLKFESVSDKIPLRFDADKMEKIICNLVSNAFKFTSENGEIILAVN
                     IVSGNVEIRVKDTGTGIPTESIPHIFNRFYQVDGSSTREQEGTGIGLALTKELVELHN
                     GHISVTSKEGVGTEFIINFPLGELKVDDNHLAKQSEDQFTFNDNFDFQESTYSESTAV
                     NNHSEFDVQRSEIILVVEDNADVRAYICEQLEKEYKLIEASNGEEGFKKAQVEIPDLI
                     ITDVMMPKLDGYRFSKLIRSDEKTSHIPIVMLTAKAGLDDKIEGLETGIDAYLTKPFS
                     AKELKATVRNLINQRIQLRKRFSKSTIIKPSEVTTISLDQEFLEKVIKVIETHYEDEQ
                     FSVDKLSEHMNMSVSQLNRKLNALIDQPPGHLIRSLRLQRAADLLKQNSGTVAEICYK
                     VGFNDQAYFSRAFKKQFGVSPTEYKSVDRD"
BASE COUNT          564 a          346 c          282 g          639 t
ORIGIN      
        1 ccgcttcatt aattaactag acataaataa catttaaaat ttaatccctg tctacactct
       61 tatactcagt gggggagacg ccaaactgtt ttttgaaggc tcttgaaaaa tatgcctgat
      121 cattaaatcc aactttatag caaatctctg caactgtacc ggaattttgc tttaaaagat
      181 ctgctgctct ctgaaggcga agtgaacgga ttaagtgtcc tggcggctga tctataagtg
      241 cattaagttt tctatttaac tgtgatacac tcatattcat atgttcggaa agtttatcga
      301 cgctgaattg ttcatcctca taatgagtct caataacttt aataactttt tctaaaaatt
      361 cctgatcaag tgaaattgtt gtgacttcag aaggcttaat aatagtggat ttactgaaac
      421 gtttccttaa ttgaattctt tgattaatta aatttcttac tgtggctttt aattcttttg
      481 cactaaaagg ttttgtaaga tacgcatcaa ttccggtttc caacccctca atcttatcat
      541 caagtccagc tttagcagta agcataacga ttggaatatg acttgtcttt tcatcactgc
      601 gaatcaattt gctgaaccga tagccatcta attttggcat cataacatca gtaattatta
      661 aatcaggaat ttcgacttgt gcttttttaa aaccttcttc accattagag gcttcaatta
      721 atttatattc tttttcaagc tgttcacata tataagcgcg cacatcggca ttatcttcaa
      781 ccactaaaat tatttcggag cgctgcacat caaattctga atgattatta accgcagttg
      841 attcagaata agttgattcc tgaaaatcaa aattatcatt aaaagtaaac tgatcttcag
      901 attgtttcgc taaatggtta tcatcaactt ttaactctcc aagcggaaaa ttaattataa
      961 attccgttcc gacaccttct ttactggtaa cagagatatg gccgttatga agttcaacta
     1021 attcttttgt aagggctaat ccgatacccg ttccttcttg ttcacgagta cttgagccat
     1081 caacctgata aaatcgatta aagatatgcg gaatactttc tgtggggatt cctgttcccg
     1141 tatcttttac tctaatctca acattacctg aaacaatatt aactgcaaga attatctctc
     1201 cattttcaga agtaaacttg aatgcatttg aaactagatt gcagatgatt ttttccattt
     1261 tatcagcatc aaatctcaaa ggtattttat cacttactga ttcaaactta agtttaatct
     1321 tttttgaaac agcaagtgat tcaaatgaga atagaagatt ttttaaaaat gaaacaatgt
     1381 tatactgctc agctttaagt tccatactgc cggcttcaag ttttgagaga tccagcaatt
     1441 gattaatgag ggtaagaagt cttctggcat ttctatttgc aacctgtagt ttgccttttt
     1501 ctctaacatc aatatttgat cctaagacac tttcaatctg accaagtatt aatgtaagtg
     1561 gagttctgaa ttcgtgtgat atgttagcaa agaaatgcga ctttaaatta tccagatgac
     1621 gcaaagtatc tgtttcaact ttttccagtt tcaactgatt tttaagtttt agcctgttca
     1681 tctcgtaacg cctgatcagg taaagtattg aaagaatgaa cattaggtac agaagatagg
     1741 cccaccatct gctccaccac ggcggagtaa taattaactt aagagctata ccttcttcat
     1801 tccaaatacc atcattgtta gttcctttga c
//
LOCUS       DASWBY010000154         6929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_571057, whole genome shotgun sequence.
ACCESSION   DASWBY010000154 DASWBY010000000
VERSION     DASWBY010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571057"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="VF870_02855"
     CDS             <1..1536
                     /locus_tag="VF870_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9251149.1"
                     /translation="TINPVYSFSVDEGVVDETLFLSLVQFEWNDESGDLTPKPMLAST
                     WEWASDSSSIIFNLRNDVVWSDGSKFTAEDVVYSFDVYSDPIVQSRYYGAFKSLYKDY
                     EEHIDINKTFEILNPYKIKINFIQGSASSILDVVYPIIPKHIYEKLDRKNISTSEINF
                     NPVSNGAYKLKKWERNQMVVLEADKKSFLYKKGMIDEIIFKIIPDYNSRITQLKKGEV
                     DFCELVKPADTKDLKNVNHLVIETVKGREYDYLGLSNIDLEKYSKNKAVYPNKLFGSK
                     KVRKALAFAINKKEILSEYLNNYGELAVTPVSSIFKLYYDKELKPIQYDVDEAKRLLE
                     EEGWVDRNNNGVIEKNGVEFTFTLYIPSGNPLREFASTVISNNLKVIGVEMKLQKLEL
                     GAFIENLYTKKMDAWMISSYIPVPLDLKTVWYSDLEKTPNNFSSYQSKDNDKIVEELE
                     KRISDAEKKDLYIKFQKNIYQDCPVVFLYWTDNIIIHNKRLGNMTIDPFGALQKMWEW
                     KIQ"
     gene            1547..2533
                     /locus_tag="VF870_02860"
     CDS             1547..2533
                     /locus_tag="VF870_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9251150.1"
                     /translation="MKKDFYKAVLKRIASSVLVLFLLVTFIFILVRLAPGDPTQKFLS
                     PQLSPQLAEKVTKSFGLDQPIYIQYYSFILNIFSGDFGISYNYRKPVFTVVKDYFLFT
                     LIFSTISFIIQLMLGYLLAKYSFNRQGKLIDKIFSKLSIILFSVPTFVLGLILVYIFS
                     VKLDFFPTSGIKSIYNDDLSALGKLEDYFAHLILPMITLSLAGVAVFYKYLRDNLESV
                     KNSAFIVNLRANGFSEKEIFKKHLIPNAIQPLISIGGVEFGILLGGALLTEVIFSLPG
                     MGRLTVSAILNRDFPLVIACSFIAGLMMILSNLAADLIKIKMDKRFIKDLIN"
     gene            2620..3813
                     /locus_tag="VF870_02865"
     CDS             2620..3813
                     /locus_tag="VF870_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9251151.1"
                     /translation="MSLFILLIQSLFVNAKNVYSQINFSLADSISALLLIIVVPVLVL
                     SSKNVKKLFSQQLTISNLILTLLIICFLFAPLITTQHPDFQKNISVTKLLPPFSSVNY
                     FELNEQSLKSVNNNSELRSLIDKTIPNSFNENIIFIDSAVVDSEFKYYQSGAQFQLEK
                     NKVKILDNQPVIKTKLFILGSDEFGRDVFTRIVYGSRISLLVGFGAVLVSFILGLSFA
                     FIAVERGGFVNLFLSRLTDLFLSFPAIFLVILIIALFGSNIFSIIIVLGFSGWMSLFK
                     VAKSEMLSVKNKEFYLSAKLIGLKNIKLLIREILPAIIIPISVNLIFQLSNVILAESA
                     LSYLGLGTGNEYPSWGAMIEAGQEYITQSWWLIFIPGLILVISLLSINEVGRNINKNL
                     NPAIK"
     gene            3810..>6929
                     /locus_tag="VF870_02870"
     CDS             3810..>6929
                     /locus_tag="VF870_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9251152.1"
                     /translation="MINKRYIVRKKLGEGRSKVFNVIDTEFPEREVAAKFLPPSASND
                     EKEFFREEYFILQRLDHPNIIKSFELSSVLVKDEDEDLEIENLSPFITMEYFSPTLLL
                     SYAGLKEERKLNQIIKQICSVLYYLHQSNYIYYDLKAENILISESNGNPTIKIIDLGF
                     ARNTLLNYDKNIQGTPYYIAPELLKNEQHNYKVDFYSLGMLLYRIVYGKFPFTSENEL
                     DIYKAHIEEEFPLDSSIYSKKLINVISKLIKKNPDDRYENALQIILDLQLPIDFEVVK
                     DLVPAKVFSDRKDAYNILNTYLKDQGSNEVFTVTGFDGSGKTSLLLEIFRQKQFSIYV
                     ENTKIKTGLEAVKYIFHKIILTEAVYSENVNEYSKIINDVFDKSSVSFLESVKRIFNT
                     LPAGVNLTVLLDDYNLYDDFTKESLTEMIRIFQVKGIKIILTESSDFDHSSSSLNNLC
                     DIQLNQFTDHQLTEFLDLSYSPLFPKQELKKYILLYSDLQPGNIKQFIKDLILLKVVK
                     FDNAKVTFESSEDIILALQSSHEELYRMRLSNLNSTELKLAQIISAFEISVEQTILAA
                     LLNVSQEILKSILNELEKKNIIDPLSLSNAPRINSFNFKKYIYSTINNRIKFHIVLAN
                     SIKKLFADFNTMELARQFELANEHEQSVEFLKKEILQAEEISAYAYKRSLLEKALRFN
                     IHEKTLSYLSLELVKTLYKLNDYKAALESIYKLNTSNYSDEDNNQLQFIKGSSLIGLR
                     KFEEGKSVLLQLQSIVNDFRLKQKIIVELAYAEFDLNNIDFAEDYCERLLEDRNLQDE
                     EKEKCYNLLGIIEIQFRNNPEKALNKFILAEKIYSSSQMANRVVRIKANIGNIYNILG
                     DRKNAEKYWNDAIEINRTIGNLSQEALILLSYGVFYQENFDFEKAKESWLRSEKIFTA
                     LGNQDKIGITLSNLGEVYLQTCDYQNAYENLIKSFNIFNELNNKEEALSALYLLGKFW
                     FVIGEIKELDKTINQYEFLLLTQETHSEKNSLNFDYLKLLFKLNTNNSFDPNYDLVPV
                     LEKANSISEL"
BASE COUNT         2490 a          951 c         1125 g         2363 t
ORIGIN      
        1 actattaatc ctgtttattc attcagcgtt gatgaagggg tagtagatga aactttattc
       61 ttgagtttag tacaatttga gtggaatgat gaatcaggtg atttaactcc caaaccaatg
      121 cttgcatcaa cctgggaatg ggcatcagac tccagttcaa tcatttttaa tcttcgcaat
      181 gatgttgttt ggagtgatgg cagcaaattt actgctgaag atgttgttta ctcatttgat
      241 gtttattccg acccaatagt acaaagcaga tattatggag catttaaaag tttatataaa
      301 gattatgaag aacatatcga tataaataag acatttgaga tactgaatcc gtataaaata
      361 aaaataaatt ttatacaggg atctgcttct tctatactgg atgttgttta tccgataatt
      421 cccaaacaca tttatgagaa gcttgataga aaaaatatct caacttcgga aattaatttt
      481 aatcccgttt ctaatggtgc gtacaagtta aaaaaatggg aacgaaatca aatggtggtg
      541 cttgaagcag ataaaaaatc ctttttgtat aaaaagggaa tgattgatga aataatattt
      601 aaaatcattc cggattataa ttcaagaatc acacaactaa agaaaggtga agtggatttt
      661 tgtgaattgg taaaaccagc tgataccaaa gatttgaaaa acgtaaacca tcttgtaatt
      721 gaaaccgtaa agggaagaga gtacgattac ttaggtttaa gcaatattga ccttgaaaag
      781 tattctaaaa ataaagctgt atatcccaat aaactttttg gcagcaaaaa agttcggaag
      841 gctttagcat ttgccattaa taaaaaagaa atactaagcg aatatttaaa taactatggc
      901 gaacttgctg ttactccggt ttcttcaatc ttcaaacttt attatgataa agaattaaaa
      961 ccaattcaat acgatgttga tgaggctaaa agattattgg aagaagaagg ctgggttgat
     1021 agaaacaata atggtgtgat tgaaaaaaat ggtgttgaat ttacttttac attatatatc
     1081 ccaagcggaa atcctttacg tgagtttgct tcaactgtta tatcaaataa cttaaaagtc
     1141 attggtgttg aaatgaaact ccagaaactt gaactgggtg catttattga aaatctttat
     1201 acaaaaaaaa tggatgcctg gatgatatca tcatatattc cggttccact agatttaaaa
     1261 actgtatggt attcggatct cgaaaaaaca cctaataatt tttcgtctta tcaaagcaag
     1321 gacaatgaca aaatagttga agaattagaa aaaagaatta gtgatgccga aaaaaaggat
     1381 ctttatataa aatttcaaaa aaatatttat caggattgcc ctgttgtatt tttgtactgg
     1441 acagataata ttattatcca caacaaaagg ttagggaata tgactataga tccgtttggt
     1501 gctctgcaga aaatgtggga atggaaaata caatagtgaa tcgtgtatga aaaaagattt
     1561 ttataaagct gtactaaaaa gaattgcaag ttcagtgctt gtacttttct tattagttac
     1621 atttattttt attcttgtaa gattagctcc gggtgatccg actcaaaaat ttctttctcc
     1681 gcaattaagt ccgcagttag cagaaaaagt aacaaaatca tttgggttag accagccaat
     1741 ttatatacag tattattctt ttattttaaa tattttttcg ggtgattttg gaatttcata
     1801 caactatcgt aagcctgtat ttacagtggt taaagactat ttccttttta ctttaatatt
     1861 ttcaacgata agttttataa ttcaattgat gcttggatat ttgctggcaa agtactcatt
     1921 caacagacaa ggtaaattaa tcgataaaat attttctaag ctttcaatta ttcttttttc
     1981 tgtaccaact tttgttttag gattaattct ggtttatata ttttctgtta agctggattt
     2041 ctttcctaca tccggaatca aatcaatata taatgatgat ttaagcgcac taggtaaact
     2101 agaagattat tttgcgcatt taatattacc gatgataaca ctttcattag ctggtgtggc
     2161 tgttttttac aaatacttaa gagataattt agagtcagta aaaaatagtg cgtttatagt
     2221 aaatttaaga gcgaacgggt tttcagaaaa agaaatattt aaaaaacatt taattccaaa
     2281 tgcgattcag cctttgattt caataggagg agttgagttt ggtattttgc ttggcggtgc
     2341 tttacttacc gaagtaattt tttctttacc aggaatgggt agattaacgg tcagtgcaat
     2401 tttaaatcgt gattttcctc ttgttatcgc ctgcagtttt attgctggat tgatgatgat
     2461 actatcaaac ctggctgctg atctcattaa aataaaaatg gataaaagat ttattaagga
     2521 tttaatcaac tagtgatatc taaaattaaa atatggcatt ttattctttt tattattgca
     2581 ctaataataa tatttagatt ttctcattta attgatttag tgagtttatt tatattattg
     2641 attcaatctc tatttgtaaa tgctaaaaat gtttattctc aaattaattt ttcattggca
     2701 gatagtattt ctgctttgtt gctaattata gttgtaccgg ttcttgtatt aagttcaaaa
     2761 aatgtcaaaa aattattttc acaacaatta acaatttcaa atttaatatt aacacttctg
     2821 atcatctgtt ttttatttgc accacttatt acgacacaac atccggattt tcagaagaat
     2881 atttcggtaa caaaacttct ccctcctttt tcatctgtaa attattttga actaaatgaa
     2941 caatcattaa aatcggtaaa caataatagt gagttgagat cgttaattga taaaacgatt
     3001 ccaaattcat ttaatgaaaa tattattttt atagatagtg ctgttgttga ttcagaattt
     3061 aagtattacc aatcaggggc tcaattccag ttagagaaaa acaaagtaaa aattttggac
     3121 aatcagccag tgataaagac aaaactattt atactgggtt cagatgaatt tggtcgagat
     3181 gtttttacaa gaatagttta tggatccaga atttctttgc ttgttggatt tggtgccgtc
     3241 ttagtgtcat ttattcttgg attatctttt gcatttattg ctgttgagcg aggtggattt
     3301 gtaaatctat ttttaagcag acttactgat ttgtttcttt cttttcctgc aatatttttg
     3361 gtcattctta ttatcgcttt atttggaagc aacatattct cgattataat tgtattgggt
     3421 ttttccggct ggatgagttt atttaaagtt gcaaagagtg aaatgctatc agtaaaaaat
     3481 aaagagttct acttatcagc taaattaatt ggattaaaaa acatcaaact acttataaga
     3541 gaaatattgc cggctataat tattccgata agtgttaact taatttttca acttagcaat
     3601 gtaattttag ctgaatccgc attgagttat ttaggtttag gaaccggaaa tgaatatcca
     3661 tcgtggggag caatgattga agcaggacag gaatacataa cacaatcatg gtggttgatt
     3721 tttattcccg gattaattct tgtaatatct cttctaagta ttaatgaagt tggcagaaac
     3781 atcaataaaa atcttaatcc tgcaattaaa tgattaataa aagatacata gtaagaaaaa
     3841 aacttggtga aggaagaagc aaagtattta atgtaattga tactgagttt cctgaaagag
     3901 aagttgcagc aaaatttctg cctccatcag catcaaatga tgaaaaggag tttttccgcg
     3961 aagaatattt tattcttcag agactagatc atccaaatat tattaaatca tttgaactta
     4021 gcagtgtttt agtaaaagat gaagatgaag atcttgagat agaaaattta tctcctttta
     4081 taactatgga gtatttttct ccaactttac ttttaagtta tgcgggatta aaagaagaaa
     4141 gaaaactaaa tcaaataata aaacaaattt gctccgttct ttattatctt catcaatcta
     4201 actacatcta ttacgatctt aaagcagaaa acattctcat atctgaatca aacggcaatc
     4261 caacaataaa aataattgat cttggatttg ccagaaacac acttttaaat tatgataaga
     4321 atattcaggg tacaccatat tacatagcac cagagctatt aaaaaatgaa cagcataatt
     4381 ataaagttga tttttattca ttaggcatgc tgctttatag gattgtgtat ggaaaatttc
     4441 ctttcacgag tgagaatgaa ttagatattt ataaagcgca tattgaagag gaatttccat
     4501 tggattcctc tatttattct aaaaagttaa ttaatgttat ttcaaaatta attaagaaaa
     4561 atcccgatga tcggtatgaa aatgcacttc aaataattct ggatttacaa cttccaatcg
     4621 attttgaagt agttaaggat ttggttccgg ctaaagtttt tagtgatagg aaagatgctt
     4681 ataatatttt aaatacctat ttaaaagatc aaggaagtaa tgaggttttt acagttaccg
     4741 gttttgatgg atctggaaaa acatcgttgc tgcttgaaat attccggcag aaacagtttt
     4801 ctatctatgt tgaaaataca aaaataaaaa caggccttga ggcagttaaa tatatatttc
     4861 ataaaataat tttaacggaa gctgtttata gtgagaacgt aaacgagtat tccaaaatta
     4921 tcaacgatgt ttttgataaa tcatcagtca gttttttaga atcagtcaaa agaatattta
     4981 atacattacc cgccggtgta aaccttacag tacttttgga tgactacaat ctttacgatg
     5041 attttacaaa agaatctctc acagaaatga taagaatatt ccaggttaaa ggtattaaaa
     5101 taattttgac agaatcatct gactttgatc attcatcctc atctttaaat aatctgtgcg
     5161 atattcaatt aaatcagttt acagatcacc agcttaccga gtttttggat ttgagctatt
     5221 ctccattatt tccaaagcag gaactaaaaa aatacatttt actgtattct gacctgcagc
     5281 ccggaaacat taagcaattc attaaagact taattctttt aaaagtagta aaatttgata
     5341 atgcaaaagt aacattcgaa tcaagtgaag atataattct tgccttacaa agctcacatg
     5401 aagaacttta cagaatgcgt ttgagtaatc tgaatagtac tgaattaaaa cttgcccaga
     5461 taatatcagc ttttgagata tctgttgaac aaacaatact tgccgcgctg ctcaatgtat
     5521 cacaggaaat actaaagtca attctaaacg aattggagaa aaaaaatata atcgaccctc
     5581 ttagtcttag taatgcacct agaataaatt cgtttaactt taaaaaatat atctattcca
     5641 caataaataa ccggattaaa tttcacattg ttctggcaaa ctcaataaag aaattgtttg
     5701 ctgattttaa cacaatggaa ttagcaagac agtttgaact tgccaacgaa catgaacaat
     5761 ctgttgaatt tttgaagaaa gaaatcctgc aagctgaaga aattagcgct tatgcttaca
     5821 aacgttcatt actagagaaa gctttacggt ttaatattca cgaaaagact ttatcatatt
     5881 taagcttaga actcgtaaaa actctttaca aactaaatga ttataaagca gcattagaat
     5941 ccatttataa gctgaacacc tctaattatt ccgatgagga taataatcaa ttacaattta
     6001 ttaaaggcag cagtttaata gggcttagaa agtttgagga gggtaaatca gtattattac
     6061 aattgcaatc aatagtaaac gactttagat taaaacaaaa aattattgtt gagttagctt
     6121 atgcagagtt cgatctaaat aatatagatt ttgcagaaga ctattgtgaa agattacttg
     6181 aagaccggaa tctacaggat gaagagaaag aaaagtgtta taatctttta ggtataatag
     6241 aaatacaatt cagaaacaat cctgaaaaag cattaaataa atttattctt gctgagaaaa
     6301 tctattcatc ctcccaaatg gcaaacagag tcgtacgcat taaggctaat attggcaata
     6361 tctataatat actaggtgat aggaaaaatg ctgagaaata ctggaatgat gccatcgaaa
     6421 taaatagaac tattggcaat ctttcacaag aagcacttat ccttctaagt tatggagtgt
     6481 tttatcagga aaattttgat tttgaaaagg cgaaagaaag ctggctgcgt tcagaaaaaa
     6541 tttttactgc tcttggaaat caggataaaa taggtattac tttaagtaat cttggggagg
     6601 tttatcttca gacgtgtgat taccaaaatg cttatgagaa tttaattaaa tcatttaata
     6661 tttttaatga gttaaataat aaagaagagg cacttagcgc cctttattta ttgggtaaat
     6721 tctggtttgt tatcggtgaa atcaaggaac tggataaaac aattaatcaa tacgaattct
     6781 tattattaac tcaagaaacg cattctgaaa aaaattcttt gaatttcgat tacttaaaat
     6841 tactgtttaa attaaatact aataattcct ttgatcccaa ttatgacttg gtacctgttt
     6901 tagagaaagc aaatagtatt tcagaactt
//
LOCUS       DASWBY010000155         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_268369, whole genome shotgun sequence.
ACCESSION   DASWBY010000155 DASWBY010000000
VERSION     DASWBY010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268369"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VF870_02875"
     CDS             complement(<1..315)
                     /locus_tag="VF870_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251153.1"
                     /translation="MKNLKYFYVSVLVLVFISCSPSQKVLSSWVNKEELKGKKYSKVF
                     VMALTQNQSSRTIVEFDLASTLKERGFESVKSIDALAGTFRENSNPTKDDILAKVREL
                     KCD"
     gene            complement(435..1784)
                     /locus_tag="VF870_02880"
     CDS             complement(435..1784)
                     /locus_tag="VF870_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251154.1"
                     /translation="MKSFLFTILVFIMLSSIYAQDSHYWNLQYGTKSTLLGGAVIGSV
                     SELSATYYNPGAVALFNDPKLILSAKVYQYEAITVVNGAGENKDLDYSSISPAPTFVA
                     FDIIIDTTGRNKLAFSLLTRQSMNFEFETRQIGIENDAIPAANFVAGGLTMNQKFDEI
                     WGGITFSHKISEIVGIGATAYVAYRNQTFSFQTVIENLDSINQISSITAIRNLKFKNY
                     RTLLKTGVGINLNPITLGLTITTPSLSLLGNGSYGYNNFINNPYDPTENVYESNYQDD
                     LKSTYKTSWSVGFGAAYWGKSLSVHFSAEWYDAVKKYNPIKLDPLYSQSREEYFTREI
                     SQQFKSIINAGIGIDYKLNDKFSLAGSFITDFSANDTNAESNISLSRWDIYHISGGSY
                     FNIGSSEITLGLSYSFGNDVIRQIADIGNPDNGTGDRVKTNSDVRFTRIKILFGFIF"
     gene            complement(1963..2544)
                     /locus_tag="VF870_02885"
     CDS             complement(1963..2544)
                     /locus_tag="VF870_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9251155.1"
                     /translation="MQLQKILIGVFLLSCVTFGQMTDTTYSLSAELGAGYSRYFTTMD
                     YNDLNKNGFTGTVRVMWNPEHLLSIGLESGYQYLYSIDISDYSTEYGNTNFSASMYTV
                     PIFIVYAMKIAPNVKLTAASGMYLLYNSGEIFGEQLKSNQISIGARAGISYTYPINNT
                     ISIGGELQYSYISKIQDQTVSIQFMFIYDFLKY"
     gene            complement(2558..>3084)
                     /locus_tag="VF870_02890"
     CDS             complement(2558..>3084)
                     /locus_tag="VF870_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251156.1"
                     /translation="EDFTWAQLRTLVTLRNGEKIPSLREALNFVLEKTTIRAVWLDTK
                     DVDVIDSSIAIQHDILQRASDMGRDLKIVIGIPADDVYEKFISQPNYQNVPSLCELST
                     DKVVQANSLIWAPRWTQGLQTAEVQQMQAQGRKVFTWTLDDANYIEEFINEGEFDGIL
                     SNYPTIVSYYHYIK"
BASE COUNT         1004 a          557 c          480 g         1043 t
ORIGIN      
        1 atcacatttt aattctctta ccttagctaa aatatcatct ttggtaggat tactattttc
       61 tctgaaggtt ccggctaaag catcaatact ttttacactc tcaaatcccc gttcctttaa
      121 tgtggaagca aggtcaaatt caacaatggt tcttgaagat tggttttggg taagtgccat
      181 aacaaaaact tttgagtact tcttcccttt caactcttct ttattaaccc acgaactaag
      241 tactttttgc gaaggactac aagaaataaa gaccaaaacc agaactgata cataaaaata
      301 ttttagattt ttcatttttc tttctaatag ataatttaaa tgtgtaaaaa aaccgccacc
      361 aatttttatt tggtggaatt aatttaaaga attaaagtat aaacaaatca gtacaaccat
      421 cataatcaat aaacttaaaa tataaaacca aacaaaattt taatgcgggt aaacctaaca
      481 tctgaattcg ttttgactct gtcccctgtt ccgttatccg gatttccgat atcggcaatt
      541 tgtctgataa catcattacc aaatgaatag gataatccaa gagtgatctc ggaactgcct
      601 atattaaaat atgaaccgcc acttatatgg tatatatccc atcttgataa agaaatattt
      661 gattcagcat ttgtatcgtt agccgaaaaa tcggtgatga aactacccgc aaggctaaac
      721 ttatcgttta acttataatc aatacctatt cctgcattta tgatactttt aaactgctgt
      781 gaaatttctc ttgtaaagta ctcttccctg ctttgagaat ataacggatc cagtttaatt
      841 ggattatatt ttttgacagc atcataccac tcagcactaa aatgtacgct taatgattta
      901 ccccagtatg cagcaccaaa tccaacagac caggatgttt tatatgttga tttaagatcg
      961 tcctgataat tgctttcata aacattttca gtaggatcat aagggttatt aataaaatta
     1021 ttatatccat aggatccatt gcctaacaga cttaagcttg gagtagtaat tgttagccct
     1081 agagttatcg gattaagatt aataccaact ccggttttaa gtaaggtacg gtaattttta
     1141 aattttagat ttctaattgc ggtgatagat gagatctgat ttatagaatc caaattttca
     1201 atgactgttt gaaagctgaa agtctgatta cgataggcaa catatgcagt tgcacctatt
     1261 ccaactattt ccgatatttt atgtgaaaaa gtaattccgc cccaaatttc atcaaatttc
     1321 tgattcatgg ttagtccgcc tgcaacaaaa ttagcagctg gaatagcgtc gttttcaatt
     1381 ccaatttgcc ttgtttcaaa ttcaaaattc attgattgtc gtgttaataa tgaaaaggca
     1441 agcttgtttc tgcctgttgt atcaataata atatcgaatg caacaaaagt tggtgctgga
     1501 gaaatacttg aatagtctaa atctttattc tcaccggctc cgttaactac agttatagcc
     1561 tcatattgat aaacttttgc agataaaatt agtttagggt cattgaataa agcaacggcg
     1621 ccgggattat aatatgtagc acttaattca ctcacgcttc caattacagc accgccgagc
     1681 agtgtagatt ttgtaccgta ttgtaaattc cagtaatgac tgtcttgcgc atatattgat
     1741 gagagcatta taaaaactaa aattgtaaat aagaacgatt tcatatttct tctctcttat
     1801 gatcgttttt ataaaaacat atttaatcac atactattta ttaaacttgt attgtatttc
     1861 aaagggaata gactgaatta ataaaaaaag cgagtttatt taactcgctt tactttattt
     1921 tcaaagacca tcagccttat tgaaaattct taattcatta atttagtact ttaagaaatc
     1981 gtagataaac ataaattgaa tggataccgt ttgatcctga atctttgaga tgtaagaata
     2041 ctgtaattca ccaccaatag agattgtatt attgattgga taagtgtaag aaattcctgc
     2101 acgcgcgcca atacttattt gattgctctt taactgctca ccaaatattt caccagagtt
     2161 gtaaagcaag tacattccgg aagcagcagt tagttttaca tttggggcta tcttcatggc
     2221 gtaaacaata aaaattggga cagtgtacat agatgctgaa aaattagtat ttccatattc
     2281 agtactgtag tcacttatat caatagaata caggtattga tagcctgatt ctaatccaat
     2341 actaagaaga tgttcaggat tccacattac tcttactgtt cctgtaaatc cattcttatt
     2401 cagatcgttg taatccattg ttgtaaagta acgcgagtaa cctgcaccaa gctcggcaga
     2461 taatgagtaa gtagtatccg tcatctgacc gaatgtaaca catgatagta gaaatacgcc
     2521 gattaatatt ttttgtaatt gcataataat tttacttcta ttttatataa tgatagtatg
     2581 atactatggt cggataattt gaaagtattc catcaaactc accttcattt ataaactctt
     2641 caatatagtt tgcatcatcc agcgtccagg tgaaaacttt tcttccctga gcctgcattt
     2701 gctgaacttc agctgtctga agcccctgtg tccatcttgg agcccatatc agagagtttg
     2761 cctgcactac tttatcagta gaaagttcac aaagagatgg aacattctga taatttggtt
     2821 gcgaaataaa tttttcatac acgtcatcag ccggtattcc aataacaatc tttaaatctc
     2881 ttcccatatc acttgctctt tgcagaatat catgctgtat tgcaattgag gagtcaataa
     2941 catcaacatc tttggtgtca agccaaaccg ctctgattgt agttttttcg agcacaaaat
     3001 tcagggcttc gcgtaaggaa ggaatctttt cgccattacg taatgttacc agagttctta
     3061 attgtgccca tgtaaaatct tcaa
//
LOCUS       DASWBY010000156         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_719749, whole genome shotgun sequence.
ACCESSION   DASWBY010000156 DASWBY010000000
VERSION     DASWBY010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719749"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..1036
                     /locus_tag="VF870_02895"
     CDS             413..1036
                     /locus_tag="VF870_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX9251157.1"
                     /translation="MKKIIFVCLFVITSGCSVFQPEEPALIQPKLLKQSELPPLTEPT
                     YSNYFEFFCEMQINANGDVEKVKLLTGSGDTKWDSLATLSLLNWKYSPAIYGGHPIKI
                     TVRRKIKVVFVEPTVFSLSEIQLDDKETADSVYQALLKGADFTSLVLKYSTSDSKTNN
                     GFIGNVNVKHFSEEISLALSQLKEDEFTKTLSYGEHYVIFKRMRLNN"
     gene            1133..1546
                     /locus_tag="VF870_02900"
     CDS             1133..1546
                     /locus_tag="VF870_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251158.1"
                     /translation="MPNKVKAVEYFYCTVEDQPGESYKLLALLAEVGVNLFAFTAVPS
                     GLLNTQFTLFPEDSNKLITEAKKASLVIHGPVPALLVQGTDELGALTEIHKKLYDYGV
                     NVVSSSAVTDGKGGFGYIIYIRTEQFNTAKAALGI"
     gene            1927..>2361
                     /gene="mraZ"
                     /locus_tag="VF870_02905"
     CDS             1927..>2361
                     /gene="mraZ"
                     /locus_tag="VF870_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX9251159.1"
                     /translation="MFRGQFTYSIDSKGRIAIPAKLRKHISADANETFVMTRGLSNCI
                     DLYPLDEWQKIEESLLNLNSFQPDDARFIRMFVQFATEDVMDGQSRIMIPSTLITYAK
                     IEKEVLILGALKKIEVWNPKVYEEYLLQSPQTYEEIAAKVMAS"
BASE COUNT          820 a          355 c          420 g          766 t
ORIGIN      
        1 agcgaaaaaa ataaattaat agtcagaaca cacagaaccc gcaaatcaac cggacgccct
       61 ttatatttaa gtaaaacaaa aagtgcaata gtctaaccac gctattgcac ttttcaattt
      121 tgtccgctct atccttcccg aagggatggc gatgccactg ggtctctttt cacaggtcag
      181 ctggacgcaa ggcaggtgaa gaatcaaggt gatccttaag atcgtccttt ttattgatgt
      241 ttaatcatac ttgctaatct gcaatcgatt cttcttgact taatcaaaaa ttaatcgcta
      301 tttacaagta gagaatttat aatttatcac aaattttgaa atgtcatcgt aattctctta
      361 aataaaaatt atttaagatg gatttgaagt cgaagtctta gagaggtata aaatgaaaaa
      421 gattattttc gtttgtcttt tcgttattac ctcgggctgt tcagtctttc aacccgaaga
      481 accagctctt atccagccga agttattaaa gcaatctgaa ttacctccat taacagaacc
      541 cacttattct aattattttg agttcttttg tgaaatgcag ataaatgcga acggcgatgt
      601 tgaaaaagta aaactcttaa ctggcagcgg agatactaaa tgggattcac ttgcaacatt
      661 atcgttgctt aactggaaat attctccggc aatttatggt gggcatccga ttaaaataac
      721 tgtgcgcaga aaaattaaag ttgtatttgt tgaaccaaca gtattttcgc tttcagaaat
      781 tcaattggat gataaagaaa ctgcagattc tgtttatcaa gctttgttaa aaggtgcaga
      841 ttttacttct ctcgttttaa aatattctac aagcgattcc aaaaccaata atggatttat
      901 cggtaatgta aatgttaaac atttttcaga agagattagc ctagcattaa gccagttaaa
      961 agaggatgag tttacaaaaa ctctgagtta tggagagcat tacgttattt ttaaaagaat
     1021 gagattgaat aattgaacgt cgtaatattc catttataga attttaaata attgtaaata
     1081 aaaattgatt agatctatta aaagtttttc aagtttttaa ggagagaaag agatgccaaa
     1141 taaagttaaa gcggtagaat atttttattg tactgttgaa gatcagccag gggagtctta
     1201 caaacttctt gccttgttag cagaggttgg agtaaatcta tttgccttta cagcggttcc
     1261 aagtggtttg ttgaacacac agtttacatt atttccagag gactcaaata aacttataac
     1321 tgaagctaaa aaagcttctt tagtaattca tggaccagta ccagcattgt tggttcaggg
     1381 aacggatgaa ttaggtgcgt tgactgagat tcataaaaag ttatatgatt acggagttaa
     1441 tgttgtttcc tcctctgcag ttactgatgg aaagggtgga ttcggatata ttatttatat
     1501 cagaacggaa caatttaata cggcaaaggc agcattaggc atttaaaata atctgcgaac
     1561 aaaaaactaa atcaaaatta aaagcagacc tcgatgaatg ctttaggagg gttttatata
     1621 aaaactaact ttgagtgatt atgaaatgac aagttgaaat aatattaatg tactcatatc
     1681 ataagcgata aaataaattt taaaattaat tgtaagaaaa ttactatcct cagtacaagt
     1741 ctatctcatt ctaaaactaa atcgtagaac tttccatcca aaatctcaat tctatttcta
     1801 agagctaacc agttaatgaa tttttataaa aatatttttt aagtttttct tgactttagc
     1861 taaatttttc cattattttg ggttgtagtg gggaaaaatg tggaattatc ccaaataaaa
     1921 aggaaaatgt ttagaggtca atttacatat tcaatagatt ctaaaggccg gattgcaatt
     1981 ccagctaaac ttagaaagca tatttctgca gatgcaaacg aaacgtttgt aatgaccaga
     2041 ggtctatcca attgtattga tttgtatcct ttggatgaat ggcagaagat agaagaatca
     2101 cttctaaact taaattcttt tcagcctgat gatgcacgtt ttattagaat gtttgtgcag
     2161 tttgctacgg aagatgttat ggatggtcaa tcaagaatta tgattccttc aacattaatt
     2221 acctatgcca aaatagaaaa agaagttttg atacttggtg ctcttaaaaa aattgaagtt
     2281 tggaatccaa aagtttatga agagtacctg ctgcaatcac cgcaaactta tgaggagatt
     2341 gcggctaaag taatggcatc t
//
LOCUS       DASWBY010000157         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_419404, whole genome shotgun sequence.
ACCESSION   DASWBY010000157 DASWBY010000000
VERSION     DASWBY010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419404"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VF870_02910"
     CDS             <1..1165
                     /locus_tag="VF870_02910"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9251160.1"
                     /translation="ELLSTPQLETSFTLPVRMDSGEVKIFQAYRIQYNYARGPAKGGI
                     RFHPEETVDTIRALACWMTWKTAVVDLPLGGGKGGVICDPKNMSDRELERLSREYVRA
                     ISPLLGVDIDIPAPDVYTNPQTMTWMMDEYETIKFKHNPGAFTDKPQQVGGTEGRRDA
                     TSRGGVISVREACNSLGFDPTKDFAIQGFGNAGQRAALLHPEILGGGRLISVSDSRGA
                     IYNKNGFDPIELVQHKLKTGTVLNFPGSKQIAHEDLLELDVEVLYPAALENAIHSKNA
                     NKIKARIVCELANGPTTPEADLILFNNGVHVIPDILGSAGGVVVSYFEMVQDKSLYFW
                     EEEEVHKRLDRKMTLAYHRVKEAIKEKKIHPRLAAMLVGVARVAEACKLRGWV"
     gene            1190..1567
                     /locus_tag="VF870_02915"
     CDS             1190..1567
                     /locus_tag="VF870_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251161.1"
                     /translation="MKYKNLNLAFLIEIFVGLGTILSISLIGPKGLAALAILAIRPFV
                     LQKEDVQDKKTYLEFSYKILSNSLSIIFIMIISIIIILQFIPLWKTKLPPVEVMFVIL
                     IPFFLLTHGVIGLINYSIFDKKE"
     gene            1674..>1873
                     /locus_tag="VF870_02920"
     CDS             1674..>1873
                     /locus_tag="VF870_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251162.1"
                     /translation="MSLTLKEISSKKDLKKFISFPYNLYKQNKYYIPPLRFDEMKTLN
                     KKENPAFDFCESKYWLVYKDNRV"
BASE COUNT          645 a          297 c          361 g          570 t
ORIGIN      
        1 agaattattg agtacaccac aacttgaaac aagttttaca cttcctgtaa gaatggatag
       61 cggagaagtg aaaatattcc aagcatacag aattcaatat aactatgcac gaggaccagc
      121 aaaaggtgga ataagatttc atccggaaga gactgtagat actattcgtg ctttggcatg
      181 ttggatgacc tggaaaactg cggtggttga tcttcctctg ggcgggggta aaggcggagt
      241 tatatgcgat cccaaaaata tgagcgacag agaattagaa agattatccc gtgaatatgt
      301 tcgtgcaatt tctccattgc ttggtgtaga tatagatatt cccgccccgg atgtttatac
      361 aaatccgcag acaatgacat ggatgatgga tgaatatgaa acgataaaat ttaaacataa
      421 tccgggtgca tttacagata aacctcaaca agtcggggga acagaaggaa gaagagatgc
      481 aacttcccgt ggtggagtta tttcagttcg cgaagcatgt aactcattag gttttgatcc
      541 aacaaaagat tttgcaatac aaggattcgg aaatgccggg cagcgcgcgg cacttttaca
      601 ccctgaaatt cttggaggcg gaagattaat ttcagtaagt gattcgcgtg gagcaattta
      661 taataaaaat ggttttgacc ctattgaatt agtacagcat aaattgaaga caggaaccgt
      721 cctgaatttt ccgggttcaa aacaaattgc acatgaagac cttcttgaac ttgatgttga
      781 agttctttac ccggcagctt tagaaaacgc cattcatagt aaaaatgcaa ataaaattaa
      841 agcaagaatt gtatgtgaac ttgcaaatgg gccaacaaca ccggaggctg atttaatact
      901 tttcaataat ggtgttcatg taattcctga tatacttggc agtgctgggg gtgtggtcgt
      961 atcatacttt gaaatggttc aggataagtc tctttatttt tgggaagaag aagaagttca
     1021 taaacgactt gatagaaaaa tgacacttgc ttatcataga gtaaaagaag caattaagga
     1081 aaagaaaata catccacgat tagctgcaat gcttgttggt gttgcacgtg ttgcagaagc
     1141 ctgcaaactt cgtggatggg tgtaattcaa taaaaagaac taatactatt tgaaatataa
     1201 aaacttaaat cttgcttttt tgatcgaaat atttgttgga ctcggaacta ttctatctat
     1261 cagtttaatt ggcccaaaag ggttggcggc tctggcaata ttagctatcc gtccatttgt
     1321 tttacagaaa gaagatgttc aggataaaaa aacttatctc gaattttcat ataaaatttt
     1381 atcaaatagt ttatcaataa tttttataat gataatttca attattatta ttctgcaatt
     1441 cattcccctt tggaaaacta aacttcctcc ggtggaggta atgtttgtaa tcctgattcc
     1501 tttttttctg cttacgcatg gtgtaattgg tttaattaat tattcaatct tcgataagaa
     1561 agaataatat ttgatttgct aataatataa gcaccgaaac attgcatttt acagccgaat
     1621 gctaaaaaat gttatattag cattaacgaa tatcaacaat aagttcctta ataatgagtc
     1681 taactctcaa agaaatatcg tctaaaaaag atcttaaaaa attcatctct tttccatata
     1741 atttatataa acaaaataaa tactatatcc cgccgctgcg ttttgatgag atgaaaacat
     1801 taaacaaaaa agaaaatcct gcttttgatt tctgtgaatc aaaatattgg ctagtttata
     1861 aagacaatag agt
//
LOCUS       DASWBY010000158         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_43072, whole genome shotgun sequence.
ACCESSION   DASWBY010000158 DASWBY010000000
VERSION     DASWBY010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43072"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VF870_02925"
     CDS             complement(<1..1059)
                     /locus_tag="VF870_02925"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9251163.1"
                     /translation="MRKILLLILCLPFFINAGTTGKLAGTIKDAQTGEPLVGANVIIE
                     GTNFGAATNVNGEYVILNISPGRYNVKFSFIGYETTIMQDVSITVDQTTILQIKLNPQ
                     TIQVEEIIVTARTPLIQKDVTSSVSVITREEIEALPVATFTELLSLQAGVSGSGSNLH
                     VRGGRSNEVAYMIDGTIVVDPLLGGLATNINNDAIQEMSLLSGTFNAEYGNSLSGVVN
                     IVTRDGADKFSAKLEARTSEFGVDQYSDLHENRVNGSISGPIVSPEYNFFVSAELNNY
                     GSYLPFGYNKASSFFSKLTTTTIPYIKISLMNRGSKGSIQNYNHSYKYIPEQYLKVRT
                     DSWQSSLNLTHTVQNNLFY"
     gene            complement(1062..1925)
                     /locus_tag="VF870_02930"
     CDS             complement(1062..1925)
                     /locus_tag="VF870_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX9251164.1"
                     /translation="MDRYKEIKEKITSKYNSLPKNQKKIADYCINNFDKIPFLNVHDL
                     SVATGASVASVVRFSQRAGFKGFSELREAITGSLQKELTNKQIFPLFEKRRVEEDLLT
                     EVANLDIKNINDTLNLIERNTFNYVIDRISKSKRVFTAGLGISYLLSEILAYQLTQVG
                     ISSSVLQHTHTVFNEHILFLNPRDLLIVFSFPPYSKETIEAAEYASKRKIEVISVTNK
                     HASPVTFFTKANLIVKSENMLFTNSFAAISVLINAIATACAIKDKQRAKKVLKESEEI
                     MINQNQVISGS"
     gene            complement(2029..>2529)
                     /locus_tag="VF870_02935"
     CDS             complement(2029..>2529)
                     /locus_tag="VF870_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251165.1"
                     /translation="YYSWLYEGTFDIDTLVTTADGGVGIIGGTPSSLQPNEAKTVYFA
                     VAVGTNETEMLANMQLAQQKYNQIVSVESDVNNIPSNFKLEQNYPNPFNPSTKISFGL
                     PQRSNVVLKVFNTLGQEVAELVNESLEAGTHSYNFDASKLTSGVYIYSLQTDAGVISK
                     KMTLIK"
BASE COUNT          794 a          438 c          426 g          871 t
ORIGIN      
        1 gtaaaataaa ttattttgta ctgtatgagt aagattcaat gaactttgcc aactatctgt
       61 tcttactttc agatactgct cggggatgta tttataagaa tgattataat tctgaatact
      121 tcctttgcta cctctgttca ttaaagaaat ttttatataa ggaattgtag ttgtagtaag
      181 tttgctaaaa aatgaacttg ctttattata tccaaatggg agataactac cgtaattatt
      241 aagttctgct gaaacgaaaa aattatattc cgggcttaca atcggaccgc tgatactacc
      301 gtttactcta ttctcatgaa gatcagagta ttgatcaact ccaaactcac ttgttcttgc
      361 ttcaagtttt gctgaaaatt tatctgctcc atccctagta acgatattta caacgccact
      421 aagtgaattt ccatattcag cgttgaacgt accgctaagt agactcattt cctgaatagc
      481 atcattatta atattagttg caagcccgcc gagcaatgga tcaacaacaa tagttccatc
      541 aatcatatat gcaacttcat ttgatcttcc gcctcttacg tgaaggtttg atccgcttcc
      601 ggaaacacct gcctgaagcg aaagtaattc tgtaaatgtg gcaacaggta atgcttcgat
      661 ttcttcacga gtaattacag atacactgct tgttacatct ttttgaataa gaggtgtacg
      721 ggcggttact attatttcct caacttgaat ggtttgcgga tttaatttga tttgaagaat
      781 tgttgtctga tcaacagtta tagatacatc ctgcattatg gttgtttcat aaccgataaa
      841 gctaaactta acattatatc tgccgggtga tatattaagt attacgtact cgccgttaac
      901 atttgtggcg gctccaaaat tcgtaccttc aattatcaca ttagcaccaa ctaaaggttc
      961 tcctgtttga gcatccttga tggtacctgc taattttccg gtagtaccgg cattaataaa
     1021 aaacggaagg caaagaatta atagtaaaat ttttctcatc attaactccc gcttattact
     1081 tgattctggt taatcataat ttcctctgat tctttaagaa ctttttttgc tctttgttta
     1141 tctttgatcg cacaggcagt agcaattgca tttatcaata cagatatggc ggcaaatgag
     1201 tttgtaaaaa gcatattctc acttttaaca ataagatttg ctttagtaaa aaaagtaacc
     1261 ggagaggcat gtttatttgt aactgagata acctctattt ttcttttgct ggcatactcg
     1321 gcagcctcga ttgtttcttt agagtaaggc ggaaatgaaa aaacaataag taaatctctt
     1381 ggatttagaa aaagtatatg ttcgttaaat actgtatggg tgtgctgcag cactgaagaa
     1441 cttattccaa cctgagttaa ttgatatgct aatatttctg aaagtaaata tgaaattccc
     1501 aatcctgcgg taaatactct ttttgatttt gagatacgat caattacata attaaatgtg
     1561 tttcgctcaa taagattaag agtgtcgtta atatttttaa tatcaagatt tgcaacttcg
     1621 gtcagaagat cttcttcaac cctgcgtttt tcaaaaagcg gaaaaatttg tttgttggta
     1681 agttcttttt gtaatgagcc tgttatagct tcacgcagtt cactaaatcc tttaaagcct
     1741 gcacgctgag agaaccttac aacagaagca acgcttgcac cagtagctac ggatagatcg
     1801 tgcacattta aaaaagggat tttatcaaaa ttattaatac agtagtcagc aatcttcttt
     1861 tgatttttcg gcaaagaatt atacttgctg gtaatttttt cttttatttc tttatatcta
     1921 tccataaaag ttatgagatt ctcagaaaat tggtatttga aaaactgtca ttcccacgaa
     1981 ggagggaatg acaaaaaatt ttaaatgtta aacaaactct tctgcctatt actttattaa
     2041 agtcattttt ttagaaatta caccagcgtc tgtctgtaat gaataaatat agactcctga
     2101 agttaattta gaagcatcaa aattatatga atgagttcca gcttcaagcg attcgtttac
     2161 aagttcagca acttcttgtc caagggtgtt aaatactttt aatacgacat tagatctctg
     2221 cggcaatcca aaagaaattt tagtagaagg attaaatgga ttcggataat tttgttcaag
     2281 tttaaaattt gatgggatgt tatttacatc agattctacg gaaacaatct gattatattt
     2341 ttgctgagca agctgcatat tggctaacat ttcagtttcg tttgtaccaa ctgcgacagc
     2401 aaaataaact gtttttgctt cattaggttg aagagatgaa ggtgtacccc ctataattcc
     2461 aacaccgcca tctgcagttg ttacaagagt gtcgatatca aaagtgcctt catacagcca
     2521 agaataata
//
LOCUS       DASWBY010000159         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_607408, whole genome shotgun sequence.
ACCESSION   DASWBY010000159 DASWBY010000000
VERSION     DASWBY010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607408"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..325)
                     /locus_tag="VF870_02940"
     CDS             complement(53..325)
                     /locus_tag="VF870_02940"
                     /inference="COORDINATES: protein motif:HMM:NF009243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HEX9251166.1"
                     /translation="MSFLEIAVNISIIMIGISLILIFIRLAIGPSIEDRIVALDLLAA
                     NAIGFVAVYAIQNKTTTFLDVGIIVALLAFLGTVAFAYYLERRTRK"
     gene            complement(322..798)
                     /locus_tag="VF870_02945"
     CDS             complement(322..798)
                     /locus_tag="VF870_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX9251167.1"
                     /translation="MKNQFLLNILLAIAWMLLSGEVTAENFIEGLIIGYLILWISKSA
                     LGGTSYFRKIPKAIGFFFYFVKELIVANLKVAFDIVTPKDYMQPGIVAVPLDAETDME
                     ITLLANLITLTPGTLSLDVSKDKKVLYIHTLYLDDTEKFRAEIKNGMEKRLLEVLR"
     gene            complement(798..>1521)
                     /locus_tag="VF870_02950"
     CDS             complement(798..>1521)
                     /locus_tag="VF870_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9251168.1"
                     /translation="IFVQDTNFTHTVLLVIAGFTMLTGVLGAASQNDMRKILSFHIIS
                     QIGYMIMGLALFSPLAIAGAIFYIIHHIIVKTNLFLVSGVVDKMKGSFQLKKIGGLYK
                     YYPLLGLLFLIPALSLAGIPPLSGFWAKFVVIKAGLELNQMFIVVVALIVGLLTLFSM
                     TKIWNEAFWKDDPSGNENILDDSFSSVSISKKVLMFSPIIVLALITIVIGFNAEPFIN
                     IANNAAAQLLNPSEYITKVLGL"
BASE COUNT          532 a          287 c          241 g          461 t
ORIGIN      
        1 atcaaaatga aaaacgaacc ggaaagaatt actattccgc ttatcacttc aatcatttcc
       61 gtgttctcct ttcgaggtaa tacgcaaatg caactgtacc aagaaatgct aacaatgcca
      121 caataattcc aacatccaaa aaagttgttg ttttattttg gattgcatac actgcaacaa
      181 atcctattgc attggcagct aacaaatcaa gcgcaactat tcggtcttca atagatggac
      241 caattgcaag tctgataaaa attagtatca gcgatatacc aatcattata attgaaatgt
      301 ttactgcaat ttcgaggaaa ctcatcgcag cacctcaaga agtcttttct ccattccatt
      361 tttaatttct gctctgaact tttctgtgtc atcaagataa agtgtatgga tgtacaaaac
      421 ctttttatcc tttgaaacat caagactgag tgtgccggga gttaatgtaa ttaagtttgc
      481 aagaagcgta atttccatat cggtttctgc atctagagga actgcaacaa ttcccggttg
      541 catataatct tttggagtta ctatatcgaa agcaacttta agattagcaa ctataagttc
      601 tttaacaaag tagaaaaaga aaccgatagc ttttggaatt ttcctaaagt agctagttcc
      661 tcccaaagca gattttgaaa tccagagaat taagtaaccg ataatcagac cttcaataaa
      721 attttcggca gtaacttctc cgcttagtag catccatgca attgccaaaa gaatatttaa
      781 taaaaactgg ttcttcatta cagtcctaaa actttcgtaa tatattcact cggatttaac
      841 aactgtgcag cagcattatt tgcaatgtta ataaatggtt cagcattaaa tcctattaca
      901 attgtaatca aagctaaaac tattatcggg ctgaacatca atactttttt ggaaattgaa
      961 accgatgaaa aagaatcatc aaggatgttt tcattcccgc ttggatcatc tttccagaaa
     1021 gcctcgttcc agatttttgt cattgaaaag agagtgagta atcctacaat caatgctaca
     1081 acgacaataa acatttgatt taattctaaa cctgctttta taactacaaa ctttgcccag
     1141 aaaccagata ggggtggaat accagcaagt gaaagcgcag gaattaaaaa cagcaatcca
     1201 agcaaaggat aatatttata tagtccgcca atctttttca gctgaaagct tcctttcatt
     1261 ttatctacaa caccgctaac aaggaataag ttagtcttta caattatgtg gtgaatgata
     1321 taaaaaattg ctccagcaat tgctagtgga gaaaacaatg caagacccat aatcatataa
     1381 ccaatctggc tgatgatgtg gaatgaaaga atttttctca tatcattttg tgaagctgca
     1441 cccagaacgc ctgtgagcat tgtaaatcct gcaattacta aaagtacagt atgggtaaaa
     1501 tttgtatcct gaacaaatat t
//
LOCUS       DASWBY010000160         3304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_81057, whole genome shotgun sequence.
ACCESSION   DASWBY010000160 DASWBY010000000
VERSION     DASWBY010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81057"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="VF870_02955"
     CDS             <1..1478
                     /locus_tag="VF870_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251169.1"
                     /translation="TILFVLIMVSVVSSRPDSNGTTPGCGGSGCHTSQTGIVTATVLT
                     GNQVRITLTGTTGNVAGELVNSIGVVVAVINSTSSNPFTLNAPSAGSYTVNAGFKNPS
                     RQWGTTTVNIVAAPDAPSLLNSNPINNTQIELSWTDNSNNEDGFKIERKTTASGTYSE
                     IAQVGPNITNYNSTGLSGGTEYCYRVRSYNSAGNSLYSNESCNFTLPDLPNVPVNLVA
                     NVIQNPLSVELVWSDNSTNEDGFIIEKEMVTEAYVVIDTVPANTASYQDLDVTYQTYN
                     YRIKAFNVTGASAYSEIAQVSVPVELTSFTASVASGNVNLIWKTATETNNSGFNIEKQ
                     KLSSPQSSVGNGEWNTISFVSGKGTTTECQIYSYKDANISAGKYLYRLKQIDFNGMFE
                     YSNAIEVIVSAPDNFSLNQNYPNPFNPSTTIEFQLPKESFVSLKVYNILGVEIATLVN
                     EQRQAGVHKINFDASGLTSGLYIYKITSGGFEQTRKMMLLK"
     gene            1576..2352
                     /locus_tag="VF870_02960"
     CDS             1576..2352
                     /locus_tag="VF870_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HEX9251170.1"
                     /translation="MNLKQKLEYHYLAFDRTKLEPDPLQFLHLFGDERDIELIGLLAS
                     IFAYGNVKQIENTLKKLILILDDNPYSFISGFSTNGDSKKLSGIKHRFYTEEDIIKLF
                     FILGKEIRKYKSIKQIFLQGYNSSDRNVKSAISNFSIHILDSFKQTFGNVSSGIKFMF
                     PLPEKGSACKRMNLFLRWMVRKDELDFGLWNEIPTGKLVIPVDTHIARICRVLNLTKR
                     KNVSWQMAEEITHNLKKFNADDPVKYDFAICHIGIRKLKF"
     gene            2384..>3304
                     /locus_tag="VF870_02965"
     CDS             2384..>3304
                     /locus_tag="VF870_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251171.1"
                     /translation="MNKINEDHFPFKYLIYFILAAIVISIAGYLYYLDRKNAIEDELY
                     RHVATIKEIKLAQIEKEQLQRKKSIASYLMLPEVKNDLKTLLTKNQSPALKNKIKKWT
                     NEIKNDFTFVSINIFNKNADLLFSTDSTDGIYDHFLKHELVVMLQKDSSALSNLYLGD
                     NKTLLQAIITPIRNGKEILGYLWTEVSFFEYLYPIISYTKKETEDVEYIILKKQSDLG
                     FVLRDIQEDKEFKIRIIPISKQDKKELESFIKGKEFFKDAKFKGSKIYASVNDIPGTD
                     WILLAKINQEKVAEYTKNTAIIVSLISFLLI"
BASE COUNT         1179 a          500 c          573 g         1052 t
ORIGIN      
        1 taacaatatt gtttgtttta attatggttt cagtggtctc atctagacca gattccaacg
       61 gaactactcc cggctgcggt ggttccggtt gtcatacttc tcagacggga atcgtgactg
      121 caacggtttt aactggcaat caagtacgga taacattaac aggaactaca ggcaacgttg
      181 cgggtgaact ggtaaattca atcggagtgg tagtggcagt tataaattct accagcagta
      241 atccttttac acttaatgct ccttcggcag gcagttatac tgtcaatgcg ggttttaaaa
      301 atcctagtcg tcaatgggga acaacgactg taaacatagt tgctgcacca gatgccccaa
      361 gccttttaaa ttcaaatcca attaacaata cgcaaatcga attaagttgg actgacaact
      421 caaataatga ggatggcttt aagatcgaaa gaaaaaccac agctagcgga acttattctg
      481 aaattgcaca agttggacca aacattacaa attataatag tactggttta tcaggtggaa
      541 ctgaatattg ttatcgtgtt cgttcttata acagtgctgg aaattcatta tactcaaatg
      601 aatcctgtaa ttttaccttg cccgatttac ctaatgttcc tgtcaatctt gtggcaaatg
      661 taattcagaa tccattatcc gttgaacttg tctggtcaga taattctaca aatgaagatg
      721 gatttataat tgaaaaagaa atggtaacag aggcatatgt tgttatagat acggtgccgg
      781 caaatactgc tagttatcag gatctggatg ttacttatca aacttataat tatagaatta
      841 aggcatttaa tgttacaggt gcctctgcat atagcgagat agcacaggtt tctgtacctg
      901 ttgaattaac aagcttcact gctagcgtag cttcgggtaa tgttaatttg atatggaaga
      961 ccgcgacaga aacaaataac tcaggattta atattgaaaa acaaaaactc tccagcccgc
     1021 aatcttcagt cggcaatggg gaatggaata caattagttt tgttagtgga aaaggaacca
     1081 caactgagtg ccaaatttac tcttataaag atgcaaatat ttctgctgga aaatatttat
     1141 atagactcaa gcagattgat tttaatggta tgtttgaata ctcaaatgct atagaggtaa
     1201 tagttagtgc tccggataat ttttcactga atcaaaatta tccaaatcca tttaacccga
     1261 gtacaaccat tgaatttcag cttccaaaag aatcatttgt ttcattaaaa gtatacaata
     1321 ttcttggagt tgaaattgca actctggtaa atgaacagag acaagcagga gttcataaaa
     1381 ttaattttga tgcttccgga ttaacaagcg gtttgtacat ttataaaata acatcaggtg
     1441 gtttcgaaca gacaagaaag atgatgctgt tgaagtaaaa tataactgct ttttagttta
     1501 taaatttcag catattaaaa gacgagccaa gtgctcgtct ttttttttat ttattccttt
     1561 agcttaattt tcaatatgaa tctcaagcaa aaattagaat atcattacct ggcttttgat
     1621 agaaccaaac ttgaacctga tccgcttcag tttttacatc tgtttggtga tgaaagagat
     1681 atcgaactaa taggtctgct tgcttcaatc tttgcttatg gaaatgttaa gcagattgaa
     1741 aacactttaa aaaaattgat tttaattctt gatgataatc cttactcatt tattagtgga
     1801 ttttctacaa atggtgactc aaaaaaacta tcaggtataa agcacagatt ttatacagaa
     1861 gaggatatta ttaagctatt tttcattctc ggaaaagaga tcagaaaata taaatctatc
     1921 aagcagattt ttttgcaagg ttacaattct tctgacagaa acgtaaaaag tgccatatca
     1981 aatttttcaa ttcatatcct ggactcattt aaacaaacat ttggaaatgt ttcaagtggc
     2041 ataaaattta tgtttccttt gcctgaaaag ggtagtgcct gcaaacggat gaatttgttt
     2101 ttacgctgga tggtaagaaa agatgaactt gattttgggt tgtggaatga aattcctaca
     2161 ggtaaacttg taataccggt tgatactcat attgcacgaa tttgtagagt attaaatctt
     2221 actaaaagaa aaaatgtaag ctggcaaatg gctgaagaaa taacgcataa tcttaaaaag
     2281 ttcaatgctg atgaccctgt aaaatatgat tttgctattt gtcatattgg aataagaaaa
     2341 ttaaaatttt aagttgaaat ataaataatg gattaaaaga gcaatgaata aaattaatga
     2401 agatcatttc ccatttaagt atttaatcta ttttatttta gccgcaattg ttatttctat
     2461 tgcaggttat ctttattatc tggatagaaa aaatgcaatt gaagatgagc tttacaggca
     2521 cgttgcaacg ataaaagaaa ttaaactagc acagattgag aaggaacaac ttcaaagaaa
     2581 gaaatcaata gcttcatatt taatgcttcc cgaagtaaaa aatgatctta aaactttgtt
     2641 gacaaaaaat caatctcctg cattaaagaa taaaattaag aagtggacaa atgaaataaa
     2701 gaatgatttt acatttgtca gcataaacat ttttaataag aatgccgatc ttcttttttc
     2761 aacagacagt acagatggaa tctatgatca ttttttaaag cacgaattag ttgtaatgct
     2821 tcaaaaggat tcttctgcac tttcaaatct ttacttaggt gataataaaa cacttttaca
     2881 ggcaattatt actccaattc gaaacggcaa agaaatttta ggctacttat ggacggaagt
     2941 ttcattcttt gagtatcttt atccaataat atcctacact aagaaagaaa ctgaagatgt
     3001 tgaatacata attcttaaaa agcagagtga tcttggattt gttttaagag atatccaaga
     3061 ggataaagag tttaaaattc ggataatacc aatcagtaag caggataaaa aagagctcga
     3121 atcatttata aaaggaaaag aattctttaa agatgcaaaa tttaaaggat caaaaattta
     3181 tgcatccgta aatgatatcc ctggaactga ttggatttta cttgcaaaaa taaatcagga
     3241 aaaagttgct gaatacacta agaacactgc tataatagtc tcattaattt ctttcttatt
     3301 aatt
//
LOCUS       DASWBY010000161         3818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_231035, whole genome shotgun sequence.
ACCESSION   DASWBY010000161 DASWBY010000000
VERSION     DASWBY010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231035"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VF870_02970"
     CDS             complement(<1..510)
                     /locus_tag="VF870_02970"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEX9251172.1"
                     /translation="MNKKLLDHLQSKKLSIEERILAGKKLRDKFPRIKQGEYKPAVNR
                     MDPVSILEEQAKTRRPDLVPVRYARMLGSPFAFLRGGAAIMAADLAVNGAPTGINVQA
                     CGDMHLANFGVFASAERNLVFGINDFDETLPGPWEWDLKRLVASVVAAGRFLGANKVK
                     CEESVMSAVS"
     gene            complement(625..3024)
                     /locus_tag="VF870_02975"
     CDS             complement(625..3024)
                     /locus_tag="VF870_02975"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX9251173.1"
                     /translation="MLNRNLFRLVKRFKYLIVLILFLLPSITILQSCTNKEEGDKTSS
                     GELDRTVLPIKEPTRQTYTELDVRNATAPPRFEVKAPEGAPNIIIILIDDMGFGVSET
                     FGGPVTTPTMDNLSKNGLIYNRFHTTSLCSPTRVALLTGYNHHSNNMGCIGEAATTFP
                     GNTSVRPQTITPMAEVLRQNGYNTAAFGKYHETPPWEISIVGPQDRWPTRSGFEKFYG
                     FIGGETNQWAPLVYDGVTLVDLPEDPNYHFTTDMTNQAISWVRYQKALAPDKPFFIYY
                     APGATHAPHQVPKKWIEKYKGKFDQGWDKMREWTLERQKKLGIIPENTKLAPKPKDIK
                     DWDKLSADEKKLFARQMEVYAGFAEQTDYEAGRLISTIKDLGVLDNTLIIFIAGDNGA
                     SAEGQMNGMFSEMTYFNAVPETVPDMLKHYNDWGGPSTYPHFSAGWAVAMDAPFSYTK
                     QVASDFGGTRNGMLIQWPAGIKVKGGLRSQFSHVIDIAPTVYELTKIPAPKIVNGIKQ
                     DPIEGTSLAYSFNDANAEERHKVQYFEMFGNRGVYQDGWMARTIHRPAWLQKPLNTLQ
                     EDKWELYNTKEDFSLANDLSSQKPEKLKEMQSLFMKEAEKYHVLPLDDRLLERTNAEL
                     MGRPTVMGDRNTVTYGEGMKGMGVDVFIDLRNSAYTITALITVEANGNGVIVCQGGRF
                     GGLSLYMKNGRPAFTYNYLGLESTTVMAPEGLKPGNYKLVYDFKYDGGGMGKGGVATI
                     FVNDKKVVEKRIEKTQPGIFSVDDLADIGVDDGTHVADYGSSSKFNGKIEYVTIERKR
                     K"
     gene            complement(3189..>3818)
                     /locus_tag="VF870_02980"
     CDS             complement(3189..>3818)
                     /locus_tag="VF870_02980"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HEX9251174.1"
                     /translation="NIDRDKERDVNINRDINVDRDINIDRDVYVRRPVAVYPRPPYSF
                     GGYHYYAFHPYHYHPYHPFYWGPAYHPWGFFVAALAVTAIIISIDNQQYHYDEGVYYQ
                     KSGSGYTVVQAPNGATIDTLPPNTQTVIINETTNNYYYGGTFYEKSDSGYTVVPPTAG
                     SVVENLPEGGEEVKIGDVTYVKIGEVYYQPIEKDGKNMYEVVNVEKVEG"
BASE COUNT         1095 a          815 c          656 g         1252 t
ORIGIN      
        1 acttaccgct gacataactg attcttcaca cttaacttta ttggcgccta aaaatcttcc
       61 ggcagcaacg acacttgcta caagtcgttt aagatcccat tcccagggac caggcagggt
      121 ctcatcaaaa tcattaattc cgaaaacaag atttcgttca gccgaagcaa agacaccaaa
      181 gtttgccaga tgcatgtcac cgcaagcctg cacgtttatt ccggtaggcg caccatttac
      241 tgctaggtca gcagccatta tagctgcccc tccgcgcagg aaggcaaatg gagaaccaag
      301 cattcttgca tacctgactg gcacaagatc cggccgtctt gtttttgcct gttcttctaa
      361 aattgaaact ggatccattc gattgacagc aggtttatat tcaccttgct taattcgagg
      421 gaatttgtcg cgcaactttt tgccggcaag aattcgttcc tcgatactca gtttttttga
      481 ctgtagatga tccaatagtt ttttattcat gctataatcc taaaaaaatt aaattggaat
      541 aaattctgta taaaatgtta tcctattgaa atcaacaacg gtcatcaaaa tgatgaccgt
      601 tatttaatat agttcattga tcatttactt tctttttcgt tcgatggtaa cgtattcaat
      661 tttaccattg aacttagaag aagaaccgta atcagcaaca tgagtaccgt catcaactcc
      721 aatatctgca agatcatcaa ctgagaaaat tccaggttga gttttttcaa tccgtttttc
      781 cacaactttt ttgtcattta cgaaaatggt agcaacacca cctttaccca ttccaccgcc
      841 atcatactta aagtcgtaaa ccaatttgta atttcccggt tttagccctt caggcgccat
      901 tacggtcgtt gattccaaac ccagatagtt atatgtaaat gcaggtcttc catttttcat
      961 atataaagaa aggcctccaa atcgaccgcc ctggcataca attactccgt ttccattagc
     1021 ttctacagta attaaagcag taatagtata agctgaattt cttaggtcga taaagacatc
     1081 aactcccata cccttcatcc cttcaccgta agtaacagtg tttctatcac ccattacagt
     1141 tggtctgccc atcaattctg cattggttct ttccaataag cgatcatcaa gtggcagcac
     1201 atgatacttt tctgcttctt tcataaatag tgattgcatt tctttaagtt tctcaggttt
     1261 ttgtgaagaa agatcattcg caagactaaa gtcttcttta gtgttatata attcccactt
     1321 atcttcctga agagtgttaa gaggtttctg taaccaggct ggtctatgga tagttctagc
     1381 catccatcca tcctggtaaa cccctctatt accaaacatt tcaaagtatt gtactttgtg
     1441 cctttcttcc gcatttgcat cattaaatga ataagccaga cttgttcctt cgataggatc
     1501 ctgtttaata ccattaacta ttttaggtgc aggtattttt gttaattcgt aaactgtcgg
     1561 cgcaatatca atcacatgac taaactgcga acgcaatcct cctttaactt ttatcccagc
     1621 aggccactga atgagcatcc catttcttgt cccaccaaaa tcagatgcaa cctgtttagt
     1681 atatgagaaa ggtgcatcca ttgctactgc ccagccggct gagaagtgcg gatatgtgct
     1741 cggtccgccc caatcattat aatgcttaag catatccgga acagtttcag gtacagcgtt
     1801 aaaataggtc atttcactga acataccatt catttgtccc tcggcacttg caccattatc
     1861 acctgcaatg aaaatgatca aagtattatc taatacacct aaatctttaa tggttgagat
     1921 aagtcttccg gcctcgtaat cagtttgttc agcaaatcca gcataaactt ccatttgtcg
     1981 ggcaaataat ttcttctcgt ctgcagaaag tttgtcccag tcttttatat cttttggttt
     2041 tggggcaagc tttgtattct caggaattat acctagtttt ttctgtcgtt ccaaagtcca
     2101 ttcacgcatt ttatcccaac cctggtcgaa tttgcccttg tatttttcaa tccacttttt
     2161 tggaacctga tgcggagcgt gtgtagcgcc tggtgcataa taaataaaga aaggtttatc
     2221 aggggctaaa gctttttgat agcgtaccca ggaaattgct tgattggtca tgtctgttgt
     2281 aaagtgataa ttaggatctt caggaagatc aaccagtgtt actccatcat aaaccagcgg
     2341 tgcccattgg tttgtctcac caccaatgaa tccataaaat ttctcaaatc ctgaacgtgt
     2401 tggccaacgg tcctgaggac cgacaattga aatttcccaa ggtggagttt catgatattt
     2461 tccaaaagca gcagtattat aaccattctg tctcaacact tctgccatcg gcgtaatagt
     2521 ttgtggtctt accgaagtat tgccggggaa tgttgttgca gcttctccaa tacatcccat
     2581 attattgcta tgatgattat atccggttag caatgctacc ctggtggggg aacaaaggga
     2641 ggtagtatga aaacggttat agattaaacc gtttttactt agattatcca tcgttggtgt
     2701 tgtaacaggt ccaccaaaag tttcggatac cccaaatccc atatcatcaa ttaaaataat
     2761 tataatattg ggcgcgcctt caggggcttt tacttcaaaa cggggaggtg ccgttgcatt
     2821 acgcacatca agttcagtgt aagtttgacg tgttggttct ttaattggca gaactgttct
     2881 gtctaattcg ccactgcttg ttttgtctcc ttcctcttta ttagtgcagg attgaagtat
     2941 tgtaatactg ggaagcagga ataagatcaa tacaattaag tatttaaatc ttttcacgag
     3001 gcgaaataga tttctgttaa gcatagtctc tcctaaataa gagtataaaa agttgagaaa
     3061 gcattttatt aattctttct taactaatca tttattgttg aagtttaaaa tatttaaact
     3121 agtgttataa attacttatt catttcattc atcacctaat acttttttag ttgatttaaa
     3181 taaaatattt atccttcaac cttctcaaca tttacaactt catacatatt tttcccatct
     3241 ttttcaatgg gttgatagta aacttctccg attttaacat atgtaacatc accaatctta
     3301 acctcttcac cgccttcagg taagttttct acaacacttc cagcagttgg cggaacaact
     3361 gtatatccgc tatcagattt ttcgtaaaaa gtacctccat agtaataatt atttgtggtt
     3421 tcatttatta taaccgtttg cgtattgggt ggaagggtgt caattgttgc tccgtttgga
     3481 gcttgaacaa cagtataacc gctgccactt ttttgataat aaactccttc atcgtaatga
     3541 tactgctgat tatctattga tataattatt gcagtgaccg ctaaagcagc aacaaaaaat
     3601 ccccacggat gatatgcagg accccaataa aatggatgat acggatgata atgatatgga
     3661 tgaaaagcat agtaatgata tccaccaaat gaatacggag gtcttggata tactgcaaca
     3721 gggcgtcgaa catacacatc cctgtcgata ttaatatctc tgtcaacatt aatatctcga
     3781 ttaatattta catctctctc tttgtctcgg tcgatatt
//
LOCUS       DASWBY010000162         5576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_644863, whole genome shotgun sequence.
ACCESSION   DASWBY010000162 DASWBY010000000
VERSION     DASWBY010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644863"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VF870_02985"
     CDS             complement(<1..590)
                     /locus_tag="VF870_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9251175.1"
                     /translation="MKKNISAFFVVIITILLISCSKDDAVTTSQSGNSTLSGQVKFLD
                     DSPASFAKIELKSNTSGRYIYDTCDVNGNFFFEFLYKDNYTLTFRSTSYDIYTSYVSV
                     SVAEDQNIVKDVYVRYNMLDDFATRIVSDSVFLIKMNPDGAKVGNNLDLINNLSGYYR
                     SNGEESISLGAKVYLIPASVDWNNPGADLTPEYIQS"
     gene            complement(614..1666)
                     /locus_tag="VF870_02990"
     CDS             complement(614..1666)
                     /locus_tag="VF870_02990"
                     /inference="COORDINATES: protein motif:HMM:NF039709.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HEX9251176.1"
                     /translation="MLKYLLVLITSSLILISCGDKNNGPEKNLTEKEKYAYDSTDLKT
                     EGMDTSGKPFLMEYKFKKGDKIMYRLTTISNNLQTIKMDTTISSGVNQKIIYILDLLV
                     KEVDEDGSTEAEINFNSIKLEAAANGQTFNFEAGKDLDSAKIHQFAEYQALYNNPFSA
                     RFSKKGDILEVYKADKISNKFLELKGAADTISVNDRNLIRQDLINGVLTPLITQIIRK
                     LSDKEVYKDSTWQIQQAPIPLMVYQINYTNTYKVESVEKLEDDRVAVINAGIDFKYSG
                     QSKVNQGNVFYNFEKPKSTAEGKIYFDVDRGIQIKSRTKTRLEISYTMEANTPQGKQK
                     GARSDVITNTNILELL"
     gene            complement(1676..2914)
                     /locus_tag="VF870_02995"
     CDS             complement(1676..2914)
                     /locus_tag="VF870_02995"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX9251177.1"
                     /translation="MKLRYNISLTTLLLLSLFSNTNKAQTVNGADLLFSENQNLIAGK
                     NIGVICNHTSLLNDGTHLVDALIEQKDVTVKAIFTPEHGLNGIAEAGEHIDYKKNLYK
                     EVPIISLYGKEKKPSKENLKDIDLLIYDIQDVGARFYTYISTMYYTLEAAGENNIPVI
                     ILDRPNPIGGSYIDGPVLDPNYKSFVGIAELPITYGMSVGELAKYFIGEKITPDWKNI
                     SLTVIACKNWKREIPNQFYSKWNSPSPNINSLETALVYPGTCLIEGTNISEGRGTIYP
                     FLQIGAPFFNADAIIEKLNLLKVEGAEFQAVEFVPEDIEEMATNPKFEGETCYGIKIK
                     VTDPNKFESVKLGVNLLYVLTKLYGDKINFNGQSFDRLAGSSILREQLKNRMSPDKIF
                     ATWQKDLTKFNKKINQYLLY"
     gene            complement(3087..3518)
                     /gene="ndk"
                     /locus_tag="VF870_03000"
     CDS             complement(3087..3518)
                     /gene="ndk"
                     /locus_tag="VF870_03000"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9251178.1"
                     /translation="MSNKTLAIIKPDAVNDNHIGEIISLINKAGFKIKALKMIKLSQD
                     AAKAFYEIHKEKPFYGELVEYMTSGPCVPIALEKENAVEDYRKLIGATDPAKAAEGTV
                     RKLYARSVQFNAVHGSDSDENAAREIAFFFSSKELIENYPG"
     gene            complement(3528..4574)
                     /gene="sucD"
                     /locus_tag="VF870_03005"
     CDS             complement(3528..4574)
                     /gene="sucD"
                     /locus_tag="VF870_03005"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX9251179.1"
                     /translation="MAIIIDKNTRLVVQGITGSEGSFHSTQMLEYGTKVVAGVTPGKG
                     GTDFQGVPVFNTVEDAVKVQKANTSVIFVPPPFAADAILEAANAGIKYIICITEGIPT
                     NDMLKVYNSIKGKDIVLVGPNCPGVISPGIAKVGIMPGFIHQKGKIGVVSRSGTLTYE
                     AVKQLSDVKLGQSTCVGIGGDPIIGSRFIDIIKMFNDDKDTDGIVMIGEIGGSAEEEA
                     AEFIKKNVKKPVVGFIAGRTAPPGRRMGHAGAIISGGKGTAAEKMAAMKKAGIHVVES
                     PAEIGVTMLKAIEKLRTKKPLVKKMAATKRTVKVVKPVKIEKPKSKKKVEKKKEDKSK
                     KHDKKSHKKKNKKK"
     gene            complement(4759..5277)
                     /locus_tag="VF870_03010"
     CDS             complement(4759..5277)
                     /locus_tag="VF870_03010"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9251180.1"
                     /translation="MKSQAEIIIRNWTKEDFPIVKNILLTTWKNTYTFIPEEDILSHF
                     EKYYSEDRLIEILNDPFSKGIIAEVNSCPAGWLKLFEDHINKKFFVSSLYVLPEYQGF
                     GIGKKLLNEAYRIAKEKQFSKVWLGVMKQNVKSLDWYTKLGFVFVEEEPFLMGSTEVM
                     HLIGYKVINSLI"
     gene            complement(5261..>5576)
                     /locus_tag="VF870_03015"
     CDS             complement(5261..>5576)
                     /locus_tag="VF870_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9251181.1"
                     /translation="RKAQKVRRLIKEDFDNAFKQVDLLITPTTPTAAFKIGEKSDDPL
                     QMYLSDIYTTSANLAGIPGMSIPIGKNSENLPIGLQILSQQFEEEKIFQLAKHIQDEI
                     SS"
BASE COUNT         1677 a         1018 c          850 g         2031 t
ORIGIN      
        1 ttcgattgaa tatattcagg cgttaaatct gcaccaggat tattccaatc aacactcgcc
       61 ggtattaagt aaacttttgc accaagggat attgattctt caccgttaga tctgtaataa
      121 ccgcttagat tgtttattag atctaaattg ttcccaactt tagctccatc cggattcatc
      181 ttaatcaaaa atacgctgtc acttacaatt ctggtggcaa aatcatcaag catattatac
      241 cttacataaa catcctttac aatattctga tcttcagcaa cacttaccga aacatagctg
      301 gtataaatat cataacttgt acttctaaat gttaaagtgt aattatcttt gtataggaat
      361 tcgaaaaaaa aatttccatt aacatcgcac gtatcataaa tgtacctgcc cgaagtgttg
      421 cttttgagtt caatttttgc aaatgaagcc ggtgagtcat cgagaaattt tacttgccct
      481 gaaagtgtgc tgttaccact ctgggatgta gtaacagcat catcttttga acacgaaatc
      541 aaaagtatag taattataac tacaaagaaa gcggaaatat tttttttcat taagtcagtt
      601 ctataaataa tacttaaagt aactctagaa tatttgtatt agtaataaca tcacttcgcg
      661 cacccttttg tttaccttgt ggtgtattcg cctccatagt gtaagatatt tctaatctgg
      721 tttttgtgcg ggatttaatc tgaatccctc tatctacatc aaaataaatt ttgccctccg
      781 cagtcgattt tggcttttcg aaattataaa aaacattacc ttgattaact tttgattgtc
      841 cggaatattt aaaatcaatt ccagcattaa tcactgcaac gcggtcgtcc tctaattttt
      901 caacactttc aactttgtaa gtatttgtat aattaatttg ataaaccatc aatggaatgg
      961 gtgcctgctg tatttgccat gttgaatctt tatatacttc tttatcggaa agctttctaa
     1021 ttatttgagt gattaaagga gtaagcactc cattgataag atcctgacgg attaaatttc
     1081 tatcatttac tgaaatagtg tcagccgcac ccttaagttc aagaaattta tttgatattt
     1141 tgtcagcctt atacacctct aagatatcac cctttttact aaatcgggca ctaaatggat
     1201 tattataaag agcctgatac tcagcgaatt gatggatctt agctgaatca agatctttgc
     1261 ctgcttcaaa gttaaaagtt tggccattgg cagcagcttc gagttttata gaattaaaat
     1321 taatctctgc ttctgttgat ccatcttcat caacttcctt tacaagaaga tcgaggatgt
     1381 aaattatttt ttgatttaca ccacttgaga tggtagtatc cattttaatt gtctgtaaat
     1441 tgtttgagat agtagtcaaa cgatacataa tcttatcacc ttttttaaat ttatattcca
     1501 tcaaaaaagg cttacctgaa gtatccattc cctcagtctt taaatctgta gaatcataag
     1561 catatttttc tttttcagtt aaattctttt caggtccgtt atttttatcg ccgcagctta
     1621 taagtattaa actggatgtg attagcacta ataggtattt taacattatt tcctcttaat
     1681 aaagtaagta ttgatttatt tttttattaa actttgttaa atctttttgc caggttgcaa
     1741 aaattttatc aggactcatc ctgtttttca attgttctct aaggatactt gaacctgcaa
     1801 gtctatcaaa tgactgtcca ttaaaattga ttttatcacc atacaattta gtaagaacat
     1861 acaatagatt tacacctaat tttactgact caaatttatt tggatcagta actttaattt
     1921 taattccata gcaggtttcc ccttcaaact ttggatttgt tgccatttcc tcgatgtctt
     1981 cgggaacaaa ttcaacggcc tgaaactcgg caccttctac ctttaggaga tttaattttt
     2041 caattatagc atcagcatta aaaaacggag caccgatttg caagaaggga tatatagtac
     2101 cgcggccttc agaaatattt gttccttcta tcaagcaggt acccggataa actaatgctg
     2161 tttccaaaga attaatattt ggtgatggag aattccattt tgagtaaaat tgatttggaa
     2221 tttccctttt ccagttttta caagcgatta cagttaaaga aatatttttc cagtcaggtg
     2281 ttattttttc tcctatgaaa tactttgcaa gttcaccaac gctcattcca tatgtaattg
     2341 gtaattctgc aataccaaca aatgatttat aattgggatc caatacaggc ccgtcaatat
     2401 aacttccacc aatgggattt gggcgatcta aaattattac aggtatattg ttttcacccg
     2461 ctgcttctag tgtgtaatac attgtagaaa tatatgtgta gaatcttgca ccaacgtctt
     2521 gaatatcata aatcagtaaa tcaatatctt ttaaattttc tttagatggt tttttttctt
     2581 tgccataaag tgaaattatg ggaacttctt tataaagatt ttttttataa tcaatatgct
     2641 caccggcttc agcaatgcca tttaatccat gttctggcgt aaagatagct ttaacagtca
     2701 catctttttg ttcaatcagc gcatcgacta aatgtgttcc atcattaaga agagaagtat
     2761 gattacaaat aacaccaata ttttttcctg caataagatt ttgattttct gaaaaaagta
     2821 aatcagctcc gttaactgtt tgagctttat tagtattcga aaaaagggat agtaaaagta
     2881 aagtcgttag actaatgtta tatcttaatt tcatcatagt tccatttcct aaactttagg
     2941 gtatcaatta ttttattgca ttgctttgtc attcacgtga aaacgtgaat ccaggactac
     3001 taaataaata gattcccact ttcgtgggaa tgacaaattt gttcagacta gtctttgaaa
     3061 agattacaat ttttaaaaaa atataattaa ccagggtagt tttcaatcaa ctctttactg
     3121 ctgaagaaaa atgcgatctc tcttgcggca ttttcatcag aatcagatcc atgaactgca
     3181 ttaaactgta cacttcttgc ataaagtttt ctaactgtgc cttcagcagc ttttgccgga
     3241 tcagttgctc caataagctt tctgtaatct tcaacggcat tttctttttc caaagcaatc
     3301 ggaacacaag gaccggatgt catatattca accaactcac cgtagaaagg cttttcttta
     3361 tggatttcat aaaaagcttt tgcggcatct tgagaaagtt taatcatttt taaagcttta
     3421 attttaaatc cggctttatt gattaaagaa attatttctc caatatgatt atcgtttact
     3481 gcatcaggtt taattattgc taatgttttg ttgctcaagt agaactccta tttcttttta
     3541 ttttttttct tatgtgattt tttatcgtgt ttttttgatt tgtcttcttt tttcttctca
     3601 acttttttct ttgattttgg tttttcaatc ttaaccggct taacaacttt aacggttctt
     3661 tttgttgctg ccattttttt gactaacggc tttttagttc taagtttttc tatagctttc
     3721 aacattgtta caccaatttc ggcaggactt tcaacaacat gaatcccggc tttcttcatt
     3781 gctgccattt tttctgctgc tgttcctttt ccgcctgaga taatcgcacc agcgtgaccc
     3841 attcttcttc cgggaggtgc agttcttcct gcaataaatc caactacagg tttctttaca
     3901 tttttcttga tgaactctgc agcttcctcc tcggcacttc cgccgatttc accaatcata
     3961 acaattccgt ctgtatcttt atcatcatta aacattttaa ttatatcaat aaatcttgat
     4021 ccgataattg gatcgccgcc aatgccaaca caagttgatt gaccaagttt tacatctgat
     4081 aattgtttta ctgcttcgta ggttaatgtg ccagaacgtg atacaacgcc aatttttcct
     4141 ttttgatgaa tgaagcctgg cataattccg acttttgcta ttccgggaga aattactccg
     4201 ggacaattag gaccaactaa aacaatgtct ttccctttaa ttgagttgta aactttcaac
     4261 atatcgttag ttggaattcc ttctgtgata catatgatgt atttgattcc agcatttgcg
     4321 gcttctaata ttgcatcagc agcaaatggc ggaggtacaa aaataacaga tgtatttgct
     4381 ttctgaactt ttacagcatc ttctactgtg ttaaaaaccg gaacaccctg aaaatctgtt
     4441 ccacctttac caggtgtaac accggcaaca actttggttc cgtattccag catttgggtt
     4501 gaatgaaatg aaccttcgct tcctgtaatt ccctgaacca caaggcgagt gtttttatca
     4561 ataattatag ccataaataa tccttttctg atttaaattt aatgaacaaa aataaggaaa
     4621 aactttggat aatgttaaat attaggtgaa ttaaggaatc aaatagataa gggaaaaaga
     4681 gtcatttcga ccgaagggag aaatctggtc tattacacat taaagatttc tcagtcgaat
     4741 actccttcga aatgactact aaataagaga attaataacc ttatacccaa tcaaatgcat
     4801 aacctctgta gaacccatta gaaatggttc ttcctcaaca aatacaaaac caagttttgt
     4861 ataccagtct aacgatttta cattttgttt cattacacca agccagacct tactaaattg
     4921 cttttccttt gcaattctgt atgcttcatt tagaagtttt ttaccgatac cgaatccctg
     4981 atattctggt aaaacatata atgatgaaac aaagaatttt ttattgatat gatcttcaaa
     5041 taattttaac cagccggcag ggcaggaatt aacttccgca attattcctt ttgagaatgg
     5101 atcgtttagt atttcaatta atctgtcctc actgtaatat ttttcaaagt gagatagaat
     5161 atcttcttcg ggaataaatg tgtaagtgtt tttccaggta gtaagtagaa tgttttttac
     5221 aatcgggaaa tcttcttttg tccagttacg aataattatt tcagcttgag atttcatctt
     5281 gaatatgttt tgctaactga aatatttttt cttcttcaaa ttgctgtgat aatatttgta
     5341 aaccgattgg aagattttct gagttcttac cgattggaat gctcataccc ggaataccgg
     5401 caaggtttgc tgacgttgtg tagatatcac tcaagtacat ttgcagcgga tcatcagatt
     5461 tttcgccaat cttaaatgct gcggttggag tagttggagt aatcaacaaa tcaacttgct
     5521 taaaggcatt atcaaaatct tctttgatta atcttctaac tttttgtgct ttgcgg
//
LOCUS       DASWBY010000163         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_494695, whole genome shotgun sequence.
ACCESSION   DASWBY010000163 DASWBY010000000
VERSION     DASWBY010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494695"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..1203)
                     /locus_tag="VF870_03020"
     CDS             complement(376..1203)
                     /locus_tag="VF870_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251182.1"
                     /translation="MAAPRNMQIVITKYLSLIFLLSGIMLCIAILEAFFIGRIYHNDY
                     IFLLISVILTVIGALIWHIPEHYRTYLIFDKQNNLLKISKKGRERDVKLDGVTKIISR
                     TVVSIGETKHHLILDKGDSKIELFNEQVAYRGSQWVVFSENLSTVIERPVTKEHWVED
                     FNGKLRQITQQEYAGSKSRSWKMMIIGVSPFAFAMAYKVDPSRETFYLYGVLNIIISI
                     CFPILLYAKKDKAWVDDLAQSKHLIIISVIVSTLKMTFFYVLFSFLFNGAKLPGIIR"
BASE COUNT          511 a          306 c          271 g          454 t
ORIGIN      
        1 attctatttt gggcaacctt tccgttgatt ccgagatgag ggcctctgat cctagcagat
       61 gcgacacatg agtcaataaa ttaaataaag cttagcaaaa gaatcgtgtt tgtgcaacta
      121 agatagggaa gtaatggtag gtgaaataag aaaggccaag cgcatgcact tgtcggagtt
      181 tttgcaagtc gcgacgaaat tgagactgca tgaatcaggg cattacagac tgaagaatac
      241 agaatataaa ttacattctt gcaagaagta gaaactcatt tgattcagga agaagccgat
      301 aacgaaaaac aataagaata attaaaactt cagctcagcg agaattacat agatacatgc
      361 tccacagcac tgtaatcatc ttataatacc aggcagttta gcaccgttaa ataaaaaaga
      421 aaataagaca tagaaaaaag tcattttaag agtggataca ataacactta taataattaa
      481 atgtttgctc tgtgctagat catcgaccca ggccttatct ttttttgcgt atagcaagat
      541 ggggaaacaa atgctaatta taatgttcaa tactccataa agatagaaag tttcccgcga
      601 aggatcaact ttgtatgcca tggcaaaagc aaatggcgat actcctatga tcatcatttt
      661 ccagctacga cttttagaac ctgcatattc ttgctgagtt atctgacgta atttcccatt
      721 aaaatcttca acccagtgtt cttttgtaac aggccgttca atgacggttg ataggttttc
      781 agaaaaaact acccattgac ttcctcgata agcgacttgc tcattgaaca attctatttt
      841 actgtcgcct ttgtctaaaa ttagatgatg tttagtttct ccgatggaca caaccgttct
      901 tgatattatt tttgttacgc catccagttt aacatctcgc tctctgcctt ttttcgatat
      961 cttaagaaga ttattttgtt tgtcaaatat cagataggtc ctatagtgtt ctggaatatg
     1021 ccatattagc gctcctataa cagtgaggat tacggatatt aacaaaaaaa tataatcgtt
     1081 gtgatatatc cgaccaataa agaaagcctc caaaatagca atgcaaagca taattccaga
     1141 gagaaggaaa attaaagaaa gatacttggt aatgacgatt tgcatgtttc tcggtgctgc
     1201 caatgggtga tccttatcaa tccttgatct aatcatcaac gcctcttcac ttcagggaca
     1261 atcctgtaat taagcgtctt taaaatttgc ccagcttggt tcgcttcaac aaacccatca
     1321 taacactgcc tacaaatacg acttcatcgt cgctattaga ggtgggtggt gttagttacc
     1381 gaacgaaata tataattctc catcgcggca acaatagtga acttcaggaa gttcttgtta
     1441 ccagaaaaac catatcatag tatccataat tcaaacagaa ttattcacct tcctgacaat
     1501 tctgaccacc cggccttcac ctacaagctt acatcgccat ct
//
LOCUS       DASWBY010000164         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_458975, whole genome shotgun sequence.
ACCESSION   DASWBY010000164 DASWBY010000000
VERSION     DASWBY010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458975"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF870_03025"
     CDS             <1..292
                     /locus_tag="VF870_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251183.1"
                     /translation="SDNYVSETSIEIQPGQTIVFHAVKGSFKVIFENCSGLFEVNSSN
                     IEGIIHQDSKWETPIFKLDSSITYPFKYKVFLLTSEIGTTRAPSKKIIAIVG"
     gene            319..>1945
                     /locus_tag="VF870_03030"
     CDS             319..>1945
                     /locus_tag="VF870_03030"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX9251184.1"
                     /translation="MTFIKSKCLVFEFIVFILSEIVFAQPNFKFQHINSEQGLSANTV
                     LCITQDSKGFLWIGTFEGLNRYDGYNFKIFKHNPDDTTSIGDNSIYSVFEDKSGTLWF
                     GTFEGGLNKFNRDTETFTRYVSKETDKNSLSNNIVYAICEDAKGYLWVGTAKGLNRFN
                     KKTDTFTRYLNNPSDNNSLSSSIISSLLYDDKANILWIGTKNGGLNKYDISKNKFTRF
                     QNNPENKYSISEDFINCLFKDASGVLWVGTYGGGLNKFDEKQNRFIVYNKRSEQHYIP
                     TDFVLSIDADNSGNLLLGTYEDGLIIFNPVTDKFISCHHNEGDLESLGDEMVYDIYKD
                     KTGLIWLGTWTNGINKLITNQKFKHYKHEPSNPNSLSGNGVSSICEDRNGIVWIGTEF
                     NGLNRFDIKSGIFTNYINDPNDKFSITSNGVSDIKEDRDGNLWIVADGYGFCRFDQKS
                     NRFIRYQNNPLDSNSLPIDQVSVLIEDKYGDLLVGTFGAGLIKFDKKKNTFTKIKYNP
                     DEPDDITAAGIYNLYEDKKGTIWIGTYSNGLFIF"
BASE COUNT          695 a          277 c          346 g          627 t
ORIGIN      
        1 ttcagataat tacgtttctg aaacttcaat tgaaattcaa cctggacaga ctatagtttt
       61 tcatgctgta aaaggatctt ttaaagtaat atttgaaaat tgttcgggac tttttgaagt
      121 caattcatcc aacatagagg gaattattca tcaggacagt aaatgggaaa caccaatatt
      181 taaacttgac tcttcaatta cttatccgtt taaatataaa gtatttctac taacttcaga
      241 gattggaact actagagctc cgtctaaaaa aatcattgct atagtaggtt agtttgtaat
      301 tagattttta ttaataaaat gacttttata aaatctaaat gcctagtctt tgaatttata
      361 gtttttattt tatcagaaat agtatttgct caacccaatt tcaaattcca gcacattaat
      421 tctgagcagg gactttctgc aaatactgtt ttgtgcatta cacaggatag taaaggattt
      481 ttatggattg gtacctttga aggacttaac agatatgatg gttataactt taaaatattt
      541 aagcataatc ctgatgacac aacaagcata ggtgataatt cgatttactc tgtatttgaa
      601 gataagtccg gtacactttg gttcggaacg tttgaaggag ggttaaataa atttaatcgt
      661 gatactgaaa catttactcg atatgtcagc aaagaaaccg ataaaaattc attaagcaat
      721 aatatagttt atgcaatctg tgaggatgca aaaggttatc tctgggttgg cacagcaaaa
      781 gggttaaata gatttaataa aaaaacagac accttcacaa gatatttaaa taatccttcc
      841 gacaataatt ctttaagctc cagcataata tcatctttat tatatgatga taaagcaaac
      901 atcctgtgga ttggaactaa aaatggcgga ctcaacaagt atgatatctc taaaaataaa
      961 tttacacgct ttcaaaataa tcctgaaaat aaatatagta ttagtgagga ttttattaat
     1021 tgtctcttta aagatgcttc cggagtttta tgggttggaa cttatggggg cggattaaat
     1081 aagtttgatg aaaaacaaaa tagatttatt gtttacaata aaagaagcga gcaacattat
     1141 attcccacag attttgtgct ttcaattgat gctgataatt ccggcaactt acttctcggt
     1201 acatacgaag acgggttaat catttttaat cccgtaacag ataaatttat ttcttgccat
     1261 cataatgaag gtgatcttga aagtctcggc gatgagatgg tttacgatat ttacaaagat
     1321 aaaaccggat tgatctggct gggcacttgg actaatggaa tcaataaact aattactaac
     1381 caaaagttta agcattataa acatgagccc tcaaatccga acagcttaag cggtaatggt
     1441 gttagttcaa tttgcgaaga tagaaatggg atcgtatgga taggtactga atttaatgga
     1501 ttaaacaggt ttgatataaa aagcggcatc tttaccaatt acattaatga tccaaatgat
     1561 aaattcagca taacttcaaa tggtgtttct gatattaagg aagacagaga tggcaatctc
     1621 tggatagttg cagatggtta tggtttttgc aggtttgatc aaaagagtaa tagatttatt
     1681 cgctaccaga acaatccatt ggatagtaac agcctgccga tagaccaggt atcagtttta
     1741 attgaagata aatatggtga tctgctcgtt ggaacttttg gcgcaggttt aataaaattt
     1801 gataaaaaga aaaacacatt cacaaaaatc aaatacaatc ctgatgagcc agatgatatt
     1861 acagcagcag gtatctataa tttgtatgaa gataaaaaag gaacaatatg gattggcaca
     1921 tatagcaatg gtctatttat ttttg
//
LOCUS       DASWBY010000165         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_231379, whole genome shotgun sequence.
ACCESSION   DASWBY010000165 DASWBY010000000
VERSION     DASWBY010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231379"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VF870_03035"
     CDS             complement(<1..571)
                     /locus_tag="VF870_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX9251185.1"
                     /translation="MAEQKPFVPFVSPETNMAEFTIRALIIGLVLAVVLGAANAYLGL
                     KAGMTIAATYPAAVIGMALIKLMKGTILEENFTRTVGSIGESIAAGAIFTLPAFFIAG
                     IWDPFFTPGNYLISAAIMIAGGILGIMFVALLRRVMVEDVELPFPESVAAAEIHKAGR
                     HGAGGSKFLFSAMGIGALVQSLGQFKLFAT"
     gene            complement(725..>1624)
                     /locus_tag="VF870_03040"
     CDS             complement(725..>1624)
                     /locus_tag="VF870_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9251186.1"
                     /translation="KDVEVIFSSINNPDGILFETNHKRKNGTIIPVEISAKLIKQGDE
                     EILLAIIRDNTERKKLELDLILAKDKAEEMDRLKTTFLSNMSHELNTPMSGIIGFSEL
                     LLSEMDNKNHREMAKIIHKSSKRLNETLNSILDLSKIESQKLDLKFSSVDLVTMIQEC
                     KYAFSDSVNKKGLKFIISFEQEKVFINSDSNIIYKVLCNIIDNSVKYTSEGEIKITLS
                     ETAENVVIKVTDTGIGIVEENLNQIFEPFRQGSEGLNRKYEGMGLGLTITRKYVELLG
                     GKLNIESKHGSGTNIEIVLPKNS"
BASE COUNT          509 a          314 c          235 g          566 t
ORIGIN      
        1 atgtagcaaa caatttaaac tgacctaaag attgtacgag agcacctatg cccattgcgc
       61 taaataaaaa tttagaacca ccagcgccat gtcttccggc tttatgaatt tctgcagctg
      121 caactgattc aggaaatggt aattcaacat cctcaaccat tactcttctg agcaatgcaa
      181 caaacataat tcccagaata ccgcccgcaa tcataattgc ggcagatatc aagtaattac
      241 caggagtaaa gaaaggatcc catataccag cgataaaaaa tgcaggtaaa gtgaagatag
      301 ctccagcagc aattgattct ccaattgaac caacagttct ggtaaagttt tcttctaaga
      361 tggtaccttt cattaatttg attaaagcca taccaattac agctgcggga tatgttgcag
      421 caatggtcat tccggctttt aatcctaagt aagcatttgc ggcaccaaga acaacggcaa
      481 gtaccaatcc gatgataaga gcacgaatag taaattccgc catattggtt tcaggtgaga
      541 caaaaggaac aaatggtttt tgctctgcca ttttttttct cctattactg agcaattaaa
      601 attgtggtta gagccacatt ttctctaaaa atagtgggct aaagttaagc aggaaagcct
      661 atcattctca acactttttt atctttttag aaagataatt taaaatactt ttgaatttat
      721 ttaatcacga gtttttcgga agtactatct cgatattagt tccactgcca tgtttgcttt
      781 caatatttaa ttttcctccg agcaactcaa catactttct ggtaatcgtt aacccaagac
      841 ccatcccttc gtatttcctg ttcagtccct cacttccctg cctgaatggt tcaaatatct
      901 ggttaagatt ttcttcaact ataccgatac cagtatctgt tactttaata actacatttt
      961 ctgcagtttc acttaaagtg atctttattt ccccttcact agtgtattta actgaattat
     1021 cgataatgtt gcaaagaact ttgtaaataa tattcgaatc agaattaata aaaacttttt
     1081 cctgctcaaa tgaaattata aacttcaatc cttttttatt aactgaatca gaaaatgcat
     1141 atttacattc ttgtatcatt gttaccagat caacactgct aaatttcaaa tcaagttttt
     1201 gtgattcgat ctttgaaaga tctaaaattg aattaagagt ttcatttaat ctcttactgc
     1261 ttttgtgaat tatttttgcc atttcacgat ggtttttatt atccatttca gaaagcagta
     1321 attctgaaaa accaattatt ccgctcatcg gagtattaag ttcgtgactc atatttgata
     1381 agaaagtggt ctttagtcta tccatttctt cagctttatc ttttgcaagt atcaaatcaa
     1441 gctcaagttt ttttctttca gtgttatcac gaattattgc caaaagaatt tcttcatcac
     1501 cctgcttgat tagtttagca ctaatttcaa ccggaattat tgtaccgttt tttcttttat
     1561 gatttgtttc gaatagaatt ccatcaggat tatttataga tgaaaatata acttcaacat
     1621 cttt
//
LOCUS       DASWBY010000166         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_268848, whole genome shotgun sequence.
ACCESSION   DASWBY010000166 DASWBY010000000
VERSION     DASWBY010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268848"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VF870_03045"
     CDS             complement(<1..845)
                     /locus_tag="VF870_03045"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX9251187.1"
                     /translation="MKLNLYKTNKQRSIFKIISIAFLSLAALLTLFFSILFILFLVYK
                     DDISKTVLSAVNPKINGKITFSDLSFTPFRHFPNASIKFYDLALHESKDSIYNSNKPP
                     VFDIKEAYISLNIVDLFSSKINVSDVTLEGGNLNIIVYPDSQTNLEKALKKITDKEKV
                     VKEETTDTVTDTTSGLNLHIDNLEIIDLELSLENQFKKNKVQLKINELQSEFSYSKNQ
                     IICSINLDTKIDSLIKNEKLLLSDQQINFESKLEVETDSIFVKLEEGKFSIGEAQFLF
                     NGIYDS"
     gene            complement(904..2025)
                     /locus_tag="VF870_03050"
     CDS             complement(904..2025)
                     /locus_tag="VF870_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9251188.1"
                     /translation="MKIFYTIILITFIATPTLFSQSEKSDTSVVTKPDTVGIGGVDWF
                     AYPYIFYSPETSLAFGGGGIVYFKLYDKPEAKSSSITPSFYYTINGQYDITIIPELFL
                     LDDNLKIWSKLNYSSYFDRYYGIGNNTPEIENEQYLQDNFQAQIKLQPKLFDDRLNIG
                     INYEIRNMTVADVKGNPFLENDTSLVGRNGGLTSGLGLAVAWDTRDNNFFPSSGGYYE
                     FFASNFFDFLGSDFNYNKKIIDLRHYFNPISDNVVAIQSYLLVENGTPPFYDLGLLGG
                     SKLMRGTILGRYRDKTYYTIQTEYRMPELIWKFGLILMAGFGDVAPSVGRMTISTVKP
                     TYGFGIRFRIDELQKVEIRMDVAFGKGSNGIYFDINQAF"
     gene            complement(2090..>2505)
                     /locus_tag="VF870_03055"
     CDS             complement(2090..>2505)
                     /locus_tag="VF870_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251189.1"
                     /translation="KTASVWVGYNIFGGDEFEYFVTPMIGGAFGRTNGITPGLEFKLS
                     YMGFELYSESEYLFDFESGENNFFYSWTDLTYSPLDWLWFGISGQRTRLYDTEVEVDR
                     GLILGCSYRNFEITGYFYNAFTDDVFFMLALSTNF"
BASE COUNT          838 a          443 c          386 g          838 t
ORIGIN      
        1 gaatcataaa tcccgttaaa aagaaattgc gcttcaccaa tggaaaattt tccctcttcc
       61 agtttaacaa atatcgaatc agtttcaact tctaatttcg attcaaaatt aatttgttga
      121 tcagataaca gaagcttttc atttttaatc agcgaatcaa ttttagtatc aagatttatt
      181 gagcagataa tttgattttt actgtatgaa aattctgatt gaagttcatt tatcttcagc
      241 tgcactttgt tttttttgaa ttgattttct aaactcaatt ccagatcaat tatttccaga
      301 ttatcaatgt gaagatttaa acctgatgtt gtgtcagtaa ccgtatcagt agtttcttct
      361 ttaacaacct tttctttatc tgttattttc tttaatgctt tctcaagatt tgtttggctg
      421 tcgggataaa cgataatatt cagatttcca ccttcaagag taacgtccga gacgtttatt
      481 ttggatgaaa ataaatccac tatattgaga gaaatgtaag cttctttaat atcgaacaca
      541 gggggtttgt ttgaattata aattgagtct ttactttcat gtaaagccaa atcataaaat
      601 tttatggaag catttggaaa atgtctgaaa ggtgtaaagg ataaatcaga aaacgttatc
      661 ttgccgttga tttttggatt gacagcagac agaactgtct tgctgatatc atccttgtaa
      721 acaagaaaca ggataaataa gatagagaag aataaggtta gtaaggcagc tagggataga
      781 aaagcgatac taataatttt aaaaattgat ctttgtttat tcgttttata aagatttaat
      841 ttcacaattt tatattgctt tgaaggcaaa gtttaaaaat tcctggccat agctatttat
      901 ttattaaaat gcctgattaa tatcaaaata aattccatta cttccttttc caaatgcaac
      961 atccattctt atctcaactt tttgcagttc atcaattctg aatcgtattc cgaatccata
     1021 agtcggtttt accgttgata tggtcattct tccaactgaa ggagcaacat cgccaaatcc
     1081 agccatcaat attaaaccaa atttccagat gagttctggc atcctgtatt ctgtctgaat
     1141 cgtataataa gttttatctc tgtatcttcc taaaattgtt cctctcataa gtttactgcc
     1201 gccaagtaaa cctaaatcat aaaaaggcgg tgttccattt tccacaagta gatatgattg
     1261 aattgctact acattatctg atataggatt aaagtaatgt cttagatcaa taattttttt
     1321 attgtagtta aaatcacttc ctagaaaatc gaaaaaattt gatgcaaaaa attcataata
     1381 tcctccgctt gaagggaaga aattattatc tcttgtgtcc caggcaacgg ctaaccctaa
     1441 tcccgaagta agtccgccat ttcgaccaac aagacttgta tcattttcca aaaaaggatt
     1501 tcctttaaca tctgccaccg tcatattcct gatctcatag tttattccaa tatttagtcg
     1561 atcatcaaac aatttaggct gaagctttat ctgtgcctga aaattatcct gcaggtattg
     1621 ttcattttca atttccgggg tgttatttcc aatcccgtaa tacctgtcga agtaagaaga
     1681 gtaatttaac tttgaccaaa ttttaagatt atcatccagc agaaagagct ctggtataat
     1741 tgtaatatcg tattggccat ttatcgtgta gtaaaacgat ggtgtaatgc tcgaagattt
     1801 tgcttccggt ttatcataca atttgaaata aacaattcca cctcccccaa aagccaggct
     1861 tgtttcaggg gaatagaata tatacggata agcaaaccag tctacgccgc caataccaac
     1921 tgtatcaggt ttggtgacaa cagatgtatc agatttttca gattgagaaa atagagtagg
     1981 agtagcgata aatgtaatca ggatgatggt ataaaatatt ttcatgacat attctggtta
     2041 tataagtgat attaaagtta gaaatatttg atcagcgatt taattaacat tagaaattag
     2101 tagataatgc cagcataaaa aaaacatcgt cagtaaatgc attgtaaaaa tacccggtta
     2161 tctcaaagtt acggtaagaa cagccaagta ttaaaccccg atcaacttca acttcagtat
     2221 catatagtct tgttctctgt ccgcttattc caaaccaaag ccaatccaga ggagaatatg
     2281 ttaagtctgt ccagctataa aaaaaattat tttcgcctga ttcaaaatca aataaatact
     2341 ccgactcgct gtataattca aatcccatat agctgagttt aaactccagt cccggagtaa
     2401 ttccattagt tcttccaaaa gcgccgccaa tcattggagt aacaaaatat tcaaactcat
     2461 caccaccaaa aatgttgtag ccaacccaga ctgaagcagt tttca
//
LOCUS       DASWBY010000167         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_459074, whole genome shotgun sequence.
ACCESSION   DASWBY010000167 DASWBY010000000
VERSION     DASWBY010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459074"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1419
                     /locus_tag="VF870_03060"
     CDS             46..1419
                     /locus_tag="VF870_03060"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX9251190.1"
                     /translation="MKKILLFLTLLIIPVFSQSNFDFEFDYAQFGYDTTSNYVEFYYS
                     FNQASLTAGPDKSHINLEGLLKIFIKNVSTGDTVVYNQWKVKHDVDTTDHSNQLLVGM
                     LGFVIPAGEYDCEFTASDLNKPEMFKDINEKLKVTPYLHKGLSVSDIQLASKMIQGSE
                     NTNSIFYKNTYEVLPIPNLVFGKSQPVAFFYTEIYNLQSELIKSDKIKMNMMLYNSKG
                     KLIKEKSKYLSRNVNTRVEVGSQILSNFPTDSYTFVIALIDSIGNQGISTAKKFFVYN
                     PDIVVVDTFETATTAVLSSTFGSMSEDELDDLFEKSKYLATKTEIDKYERLSNIDGKR
                     EFMFEFWKLKDETFGTAQGNNEFYRTYLQRIDLCNQRYSTMGKQGWKTDRGRVYLLYG
                     EPTEIERYPNQLETRPYEIWQYTDIEGGVYFVFADLTGFSDYTLVHSTKRGELRDDNW
                     QRRIVVK"
BASE COUNT          563 a          236 c          279 g          529 t
ORIGIN      
        1 tattgcataa caatagacgc ttttttattt aatgaaaata gcaaaatgaa aaaaatatta
       61 ttattcctta ccttattaat cattcctgta ttttcacagt caaattttga ttttgagttt
      121 gactatgctc agtttggtta tgataccact tcaaattatg ttgagtttta ttactctttc
      181 aatcaggcat ctttaactgc aggacctgat aaatcacata taaacttaga aggtttatta
      241 aaaatattta ttaaaaacgt ttctaccggt gatacagtag tgtataacca gtggaaagta
      301 aaacacgatg ttgatacaac tgatcatagt aatcaattgc tggttggtat gttaggattt
      361 gtaattccgg caggagaata tgattgtgaa tttactgcat cagatcttaa taaaccggaa
      421 atgtttaagg atataaatga aaaacttaaa gttacaccct atttgcacaa aggtttaagc
      481 gtaagtgata tacaacttgc ttccaaaatg attcagggta gtgaaaacac taactccatt
      541 ttttataaga acacgtatga agttctccct attcctaatt tagtatttgg taaatctcaa
      601 ccggttgcat ttttctatac ggaaatctac aaccttcaat cagagttgat aaaaagtgat
      661 aaaatcaaaa tgaatatgat gctttataac agcaagggaa aactaattaa agaaaagtcc
      721 aagtatcttt caagaaatgt taatacgcgt gtagaagtag gttctcaaat attatcaaat
      781 tttccaactg actcatatac ttttgttatt gcattgattg atagtatagg aaatcaagga
      841 atttctactg caaaaaagtt ttttgtgtac aatccggata ttgttgttgt tgatactttt
      901 gagactgcaa ccacagccgt actaagttct acgtttggtt caatgtctga agatgaactg
      961 gatgacctat ttgaaaaatc aaaatatctt gcaacaaaaa ctgagattga taaatatgaa
     1021 agactatcaa acatagatgg taaaagagaa tttatgtttg aattctggaa gctgaaagat
     1081 gaaacttttg gcacggcgca aggtaataat gaattttaca gaacttatct acaacgaata
     1141 gatttgtgca accaaagata ttccactatg ggcaaacaag gatggaaaac ggatagaggc
     1201 agagtttact tgctttacgg cgaaccgaca gaaattgaac ggtatcctaa tcagttagaa
     1261 accagacctt atgagatttg gcagtatact gatattgaag gtggagtcta ttttgttttt
     1321 gctgatttaa ctggcttttc agattacact ctggttcatt ctacaaagcg cggagagttg
     1381 agagatgata actggcaaag gcgaatagtc gttaaataaa atttaactaa ttttaaagcc
     1441 ccgcactgag gggctttttt attgacaaat atgtgattaa taaatgcact cattttacta
     1501 tttttaggca tcaaaatatt ttatgatgtg aaaaataccc tcgaatacat actctttctg
     1561 ttctttagtt ttctctgccg aatacttgga ttaagaatat caagaaa
//
LOCUS       DASWBY010000168         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_532462, whole genome shotgun sequence.
ACCESSION   DASWBY010000168 DASWBY010000000
VERSION     DASWBY010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532462"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /gene="ygiD"
                     /locus_tag="VF870_03065"
     CDS             complement(<1..664)
                     /gene="ygiD"
                     /locus_tag="VF870_03065"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEX9251191.1"
                     /translation="MNRKGFLKYLAMMPVTGLAMNLNDLNKITEPLDSTDKMPVLFLG
                     HGSPMNAIEENEFVQGFRNIGREIPKPNAVLVVSAHWETRGTFVTAMEKPKTIHDFGG
                     FPKELYEVQYPAPGNPELAKETKSIIKKTEIGLDLSWGLDHGAWSVVKHLYPNADVQI
                     IQMSLDYTQSPQYHYELAKELGSLRKKGVLIIGSGNMVHNLRMVDWLRLDEVDYGYDW
                     ALE"
     gene            complement(719..1603)
                     /locus_tag="VF870_03070"
     CDS             complement(719..1603)
                     /locus_tag="VF870_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9251192.1"
                     /translation="MSNINLIIEERATSIGKFVVGRLLPFRQKRMVGPFIYIDHMGPV
                     KLSRRENFDVLPHPHIGLSTLTFLFEGSIMHRDTLGNEVEIKQGAVNWMTAGKGIVHS
                     ERTPGYLRNTDKNMHGLQIWVALPKDLEQMEPEFFHIDQEQIPTWTEGDINFKLIAGE
                     AFGKKSAVPVYSKLYMIEIKSKTSQKVFIGEHLYGESGLYILEGGIESDGNIYQPKQL
                     LVAKESSLCQFTIQAGSTIYLFGGEPFPEERFIDWNFVSSRKELIEEAKQKWEAQSFP
                     KIKGDDTEFIPYPSLTKK"
     gene            complement(1648..>2023)
                     /locus_tag="VF870_03075"
     CDS             complement(1648..>2023)
                     /locus_tag="VF870_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9251193.1"
                     /translation="LLFLRITIGIFMLTHGTGKLIALFGGGPIQFADPIGIGPAASLA
                     LAVFAEFVCSLFLIFGVATRFAVIPLIITMLIAGFIIHAEDLFRVKEMAFLYLSTFIT
                     ISIAGAGKFSVDNLIYKKINLD"
BASE COUNT          603 a          426 c          339 g          655 t
ORIGIN      
        1 cttcgagtgc ccaatcatat ccgtaatcaa cttcatccaa cctaagccaa tcaaccattc
       61 ttaggttatg gaccatattt ccactaccga tgataagcac gcctttttta cgaagggatc
      121 ccagttcttt tgcaagctca tagtgatatt gaggagattg ggtataatcc agactcatct
      181 ggattatctg aacgtcagca tttgggtaaa gatgttttac aactgaccat gcaccatgat
      241 ctagtcccca gcttaaatcc aatccaattt ctgttttttt aatgatgctt tttgtttctt
      301 ttgcaagttc aggatttccg ggtgcaggat attgtacttc atacaattct tttggaaacc
      361 cgccaaagtc atgaatagtt ttcggtttct ccattgcagt cacaaatgtt cccctagttt
      421 cccaatgagc agacacaact aaaacagcat tgggtttggg aatttccctg ccgatatttc
      481 taaatccctg aacaaattca ttttcctcaa ttgcattcat tggactgccg tgaccaagaa
      541 acagcacagg cattttatct gtgctgtcaa gcggttctgt tattttattt aaatcattta
      601 ggttcattgc taatcctgtt acaggcatca ttgccagata ttttaaaaac ccttttctgt
      661 tcactctact tttataagtt tttctttatg gttaataatt acacttctga tgtaacgatt
      721 attttttagt gagtgatgga taaggaataa attcagtatc gtcacctttt attttaggaa
      781 aagattgtgc ttcccatttt tgttttgctt cttcaattag ttcttttcgg gatgaaacaa
      841 agttccagtc aataaagcgt tcttcaggaa atggctcacc tccaaataga taaatagtgc
      901 tgccagcctg aattgtaaat tggcataagc tgctttcctt agccactaat aattgttttg
      961 gttgataaat attcccatcg ctttcgattc caccttccaa aatgtataaa ccggattcgc
     1021 cgtacaaatg ctcgccaata aaaacttttt gacttgtctt acttttgatt tcaatcatat
     1081 agagtttgct ataaacagga acagctgatt ttttgccgaa cgcttctcca gctattaatt
     1141 tgaaattgat gtctccttct gtccaagtgg gaatttgttc ctggtcaata tgaaaaaact
     1201 ccggttccat ctgttcaagg tcctttggca atgccaccca gatttgtaag ccatgcatgt
     1261 ttttatcagt gtttcgtaaa tatccaggag tacgttcgga atgaacaatt cccttacctg
     1321 ctgtcatcca gttaacggca ccttgcttta tttccacttc gttacccaat gtatcccgat
     1381 gcataatgct accttcaaat aaaaaagtaa gtgtcgaaag accaatgtga ggatgtggca
     1441 gtacatcaaa attttcccgc ctgctcagtt taacgggacc catatggtca atgtaaatga
     1501 atggtcccac cattcgtttt tgtcggaatg gcagcaaacg cccaaccaca aacttaccta
     1561 tgctggtggc tctttcttct attatcaggt tgatgtttga cataataatt gttcattcaa
     1621 atttgtagga caaagtctaa tctgaattta atcaagattt atctttttat aaatcaaatt
     1681 atcaacagaa aattttccag cgcctgcaat tgagattgta ataaatgtac tcaggtaaag
     1741 aaatgccatt tctttaaccc gaaacaaatc ttcagcgtga ataataaaac cggcaatcag
     1801 cattgttata attaatggaa tgaccgcaaa tcttgtagca actccaaaaa ttaaaaacag
     1861 agaacataca aactcagcga ataccgccaa tgcaagagat gcagcaggtc caataccaat
     1921 cggatcagca aactgaatag gaccaccgcc gaacaacgca attaatttgc ccgttccgtg
     1981 cgtaagcata aaaataccaa tcgtaatgcg aagaaataat aat
//
LOCUS       DASWBY010000169         5624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_607803, whole genome shotgun sequence.
ACCESSION   DASWBY010000169 DASWBY010000000
VERSION     DASWBY010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607803"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VF870_03080"
     CDS             complement(<1..942)
                     /locus_tag="VF870_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9251194.1"
                     /translation="MKNDKTFWSDNHLGVLENVNKTEFHLLADDLPILLCRFKKDGTI
                     YFVNKAYSRFFHTAKENLLGSKFFIPPSELDKTDSQKHFLNQFKKQFSLKLNDDSIHW
                     IEWTITRIVEKNSDGFEYQAVGHDISEHKLLEEHLSKISVAVEQSKSTVVITDTGGNI
                     EYVNPRFSEVTGYTEQEVLGQNTRILKSGKSDKKIYADLWSTISNGKEWQGELLNKKK
                     NGDLFWELVSISPVKNLNGKITSYLAVKEDISEHKKSEKLEKALYQISHAVINNENLN
                     DLYSSIHQSLADVLPVENLFIALYDQQKNLLSFPYFVD"
     gene            complement(1196..2389)
                     /locus_tag="VF870_03085"
     CDS             complement(1196..2389)
                     /locus_tag="VF870_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX9251195.1"
                     /translation="MSLEIIKEKVAQAVDILNEKNIDMWVTFVQETKVLKDPIMDMTV
                     GDHSTWKSAFIINKDGDTTAIVGDMEMENFVKSGVYKNIVGYLKSFKEPFVEYVKKKN
                     PQTIAINFSVNSVLSDGLTHGMYLLLMEYLKGTGYENKIVSAEEIISPLRGRKSDKEL
                     AIMQEAVDETLKIFDMVTDFIKPGLSEKDVADFVLKVTKGKGFGLAWDEETCPAVFTG
                     PNPSNPHAGPTDRKIEKGHMINMDFGIYFKGYCSDLQRTWYVLKDGETKAPAEVQKGF
                     DIIRDSIQMVADALKPGITGVEMDDIARNYIVKNGYPEYPHGLGHQVGKEVHDGGAGL
                     FPRWEKYGNSPFLKLEERQVFTIEPRLPVEGYGVSTLEEEVYLTKTGCKFISKPQKEL
                     ILIKA"
     gene            2714..4054
                     /gene="gdhA"
                     /locus_tag="VF870_03090"
     CDS             2714..4054
                     /gene="gdhA"
                     /locus_tag="VF870_03090"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX9251196.1"
                     /translation="MSDYTNKLIADVKAKNPSEPEFHQAVEEVMGSLELVLQRHPEYR
                     SMKILERMVEPERVIMFRVPWLDDQGEIHINRGYRIEMNSAIGPYKGGLRFHPSVTLG
                     ILKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELQRHIGPDT
                     DVPAGDIGVGAREIGFLFGQYKRLRNEFTGVLTGKGINWGGSLIRPEATGFGVVYFAE
                     EMLKTKKTSFDGKIVAVSGFGNVAWGAVQKATELGGKVVTISGPDGYIYDPDGIKGEK
                     IDYMLKLRSSNKDIVEDYAKEFKVKFVPGKRPWEVKCDVALPCATQNELNADNAKELV
                     KNGCMCVCEGANMPTTLDGYKVFTDAKILYSPGKASNAGGVATSGLEMSQNSMRLPWS
                     REEVDKRLHEIMIRIHATCVTTAEKYGTPGNYVNGANIAGFLKVADAMLDQGLV"
     gene            4220..>5624
                     /locus_tag="VF870_03095"
     CDS             4220..>5624
                     /locus_tag="VF870_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9251197.1"
                     /translation="MENRSSKVRQHSIEDVFSSRIKKFQKLMRYKIREILLVSSIYDN
                     YLFEEDGRLYELIREEYQALNLSFAPELIHVTSGEEALEMLSGGNTFDLIITTLHIED
                     MHVIKFAQMVRQSGINLPIVLLAYDNRERKELTANYDTSIFERIFIWSGDYRLLIAIV
                     KYIEDRVNVENDTKNIGVQSIILVEDNIKFYSSYLPIIFSEVFKQSQRLVREGINLTH
                     KFLRMRARPKIILCTTYEEAWNYYEKYQEYVLGLILDINFKHHGVKDPEAGIKFARTV
                     RSQHDDIPILLQSSDARFREKAYEIGASFMHKNSPRLLQELREFMLQNFGFGDFVFVN
                     NAGQEVFRAANLSELEEALIKIPDESLVYHASRNHFSKWLKARTEFYLSLKLRPHKVS
                     DFGSVQGLRDRLLTSLQEYRKIQQSGIILDFNKNMFDPKNSFARIGGGSIGGKARGLG
                     FINTLIINYDLKNKFDNV"
BASE COUNT         1837 a          967 c          964 g         1856 t
ORIGIN      
        1 atctacaaag tatggaaagc ttaaaagatt tttttgctga tcatataatg caataaaaag
       61 attttctacc ggaagaacat ctgcaagaga ttggtgaatt gatgaataca gatcattaag
      121 attttcgtta tttataactg cgtgagatat ttgataaagt gctttttcta atttctcaga
      181 tttcttgtgt tcactaatat cttctttaac ggcaagatag cttgtaattt ttccattaag
      241 atttttaacc ggtgaaattg aaactagttc ccaaaaaaga tcaccattct ttttcttgtt
      301 tagtagttct ccctgccatt cttttccatt tgaaatcgta ctccaaagat cagcatatat
      361 ttttttatca ctcttgcctg atttaagtat gcgggtgttc tgccccagca cttcttgttc
      421 agtataacca gtaacttctg aaaatctggg attaacatat tcaatgtttc ccccggtatc
      481 ggtaatcaca actgtagatt tgctttgttc aacagcaaca gaaattttac ttaaatgttc
      541 ttcaagcagt ttatgttcag aaatatcatg tccgacagcc tgatattcaa atccatctga
      601 attcttttca actattctgg taatagtcca ttcaatccag tgaatcgaat catcgttaag
      661 ctttagtgaa aattgttttt tgaactgatt taaaaaatgt ttttgagaat ctgttttatc
      721 aagctcagaa ggaggaataa aaaatttgct gcctaacaaa ttttctttag ccgtgtgaaa
      781 aaatcttgag tacgctttat taacaaagta aattgtacca tcttttttaa atcggcataa
      841 tagaattgga agatcatcgg caagaagatg aaactcggtt ttgtttacat tttccaaaac
      901 ccctaaatga ttatcactcc aaaatgtctt atcgtttttc aaaatttctc actgtttatg
      961 agtcaataac tatgcatctt tgtttttagg atcacatttg cacaatattc attccattaa
     1021 gcaaaaagac acctctaaaa atggcttcat tttttttata attattaact tttattaaga
     1081 attgccgaag actgaaatga aattgctgta aaattgaccg aaactgtaga attgtaaaaa
     1141 atactataag taaaatattt ccggcttagt aattattacc aagccggaaa taaaattaag
     1201 cctttatcag gataagctct ttctgtggtt ttgatatgaa cttacatcct gttttggtta
     1261 aataaacttc ttcttcaagc gttgaaactc cgtatccttc aaccggcaat cttggttcga
     1321 ttgtaaaaac ctgacgctct tcaagtttta gaaatggaga gttaccatat ttttcccagc
     1381 gaggaaaaag tcctgcaccc ccatcgtgca cttctttacc aacttgatgc ccgagtccgt
     1441 gcggatattc cggatatcca tttttaacaa tataatttct tgcaatgtcg tccatttcaa
     1501 ctcccgttat acccggcttt aaagcatctg caaccatttg aatggaatca cgtatgatat
     1561 caaatccttt ttgtacttca gcaggagctt tggtttctcc atcttttaaa acgtaccaag
     1621 ttctctgcaa atcagaacaa taacctttaa agtaaattcc aaaatccatg ttgatcatat
     1681 gtcctttttc aatttttctg tctgttgggc cagcgtgcgg gttagatggg tttggacccg
     1741 taaaaacagc tggacaagtt tcttcatccc aagctaagcc aaatcctttt ccttttgtaa
     1801 ccttcaatac aaaatcagca acatcttttt cacttaaacc tggtttgatg aaatctgtaa
     1861 ccatatcgaa gatcttcaga gtttcatcaa ctgcttcctg cataattgct aattctttat
     1921 cggattttct ccctcgtaaa ggggaaatga tttcttctgc actaacaatt ttattttcgt
     1981 atcctgttcc tttaagatat tccatcaaca gcagatacat tccgtgtgtt aatccatctg
     2041 aaaggactga gttaacagaa aaatttattg caattgtttg gggatttttt ttcttaacat
     2101 actctacaaa cggttctttg aaagatttta agtaaccaac aatgttttta taaacaccgg
     2161 atttaacaaa gttttccatt tccatatcac caacaattgc agttgtatcg ccatctttat
     2221 ttatgataaa agctgatttc caggttgagt gatctccgac agtcatatcc ataattggat
     2281 cttttaaaac cttggtttcc tgaacaaaag taacccacat atcaatattt ttttcattta
     2341 aaatatcaac ggcttgcgca actttttcct taattatttc taaggacata ttaacctcac
     2401 ttataaattt agataaacag ttaattcaca tttattgatt gaaaaattct atcctcaaaa
     2461 taactccaaa aatcaaataa tactcctcta atccacctta attatttagg gaattactta
     2521 ttttctttct aattttgatg taataaaaat attgatttta acactaaatg ctaaacgcaa
     2581 cagtaatatt tcttagggga aaaagttttt tacacgtcga aatttttctg tccatttata
     2641 caattaataa aaaattattt atcacaaact taattcatca ttgatgaaca acaaacaaaa
     2701 atagaggtct tttatgtctg actacacaaa taaattaata gctgatgtta aagctaaaaa
     2761 tccttcagaa ccggaattcc atcaagcagt tgaagaggtg atgggttcac ttgaattagt
     2821 tttacagcgg catcccgaat atcgctcaat gaaaatttta gagcgtatgg ttgaaccgga
     2881 aagagttatt atgttccgcg ttccctggct cgatgatcag ggagaaattc acatcaatcg
     2941 tggttacaga attgaaatga attctgctat aggtccttat aaaggcggat taagatttca
     3001 cccgtctgtg acacttggta ttttaaaatt cttagcattc gaacaagtat ttaaaaattc
     3061 acttacaaca cttcctatgg gcggcggaaa aggcggatct gattttgatc ctaaaggaaa
     3121 atctgacaat gaagttatgc gcttttgcca aagctttatg actgaacttc aaagacatat
     3181 tggtcccgat actgacgtac ctgcaggaga tattggtgtt ggtgcaagag aaatcggatt
     3241 tttattcgga cagtacaaga gattaagaaa tgaatttacc ggagtattaa ccggtaaagg
     3301 aattaattgg ggtggatctc tcatcagacc tgaagctact ggttttggtg tagtatattt
     3361 tgctgaagaa atgttaaaaa ctaaaaaaac ttctttcgat ggtaaaattg ttgcggtttc
     3421 cgggttcggt aacgttgcct ggggtgccgt tcaaaaagct actgagcttg gtggaaaagt
     3481 agtcacaatt tcaggtcccg atggatacat ttatgatcct gatggaatta aaggagaaaa
     3541 aattgattac atgttaaaac tccgctcaag taataaagat attgtggaag attatgcaaa
     3601 agaatttaaa gtaaaatttg ttcctggtaa aagaccatgg gaagttaaat gcgatgttgc
     3661 tttaccttgc gcgacacaaa atgaactaaa cgcagataat gcaaaagaat tagtaaagaa
     3721 cggatgtatg tgtgtttgcg aaggtgcaaa catgccaact accctggacg gatataaagt
     3781 atttacagat gctaaaattc tttactcacc cggtaaagct tccaacgccg gtggagttgc
     3841 aacttctggt ttggagatgt ctcaaaactc tatgagatta ccatggagcc gtgaagaagt
     3901 tgataaacgt ttacacgaaa ttatgatcag aattcatgct acctgtgtta caactgcaga
     3961 aaaatatgga actccgggta attacgtaaa cggagctaac atagccggct tcttaaaagt
     4021 tgccgatgca atgcttgatc aaggacttgt ttaatttaga atgtataatt aaaaatgtac
     4081 aataaaggcg atctttggat cgcctttttt attagtagct ccattatttc aaaactccaa
     4141 cattcctttt agcaattact gacttctaac aatctattca ttatatttta accatcaata
     4201 aatcaatctc atcttattta tggaaaacag atcatcaaaa gttagacagc atagtataga
     4261 agacgtattc agttcacgaa taaaaaaatt tcagaaacta atgcgttata aaatccgtga
     4321 gatacttctt gtttcaagca tctatgataa ttatctgttt gaggaagacg gaagacttta
     4381 tgaattgatt agagaagaat atcaggcttt aaatctaagc tttgcgcccg agctgatcca
     4441 cgttacaagc ggtgaagaag ctttggagat gctaagcggt ggtaacacgt ttgatcttat
     4501 tatcaccact ctgcacattg aagatatgca cgttataaag tttgcacaaa tggtaagaca
     4561 atccggcatt aatctcccga ttgttttgct tgcttatgat aatcgtgaaa gaaaagaact
     4621 tacagcaaat tatgatactt caatttttga aagaatattt atctggagtg gagattaccg
     4681 actgcttatt gcaatcgtaa aatatattga ggaccgtgtt aatgttgaaa acgatacaaa
     4741 gaacattggt gttcaatcta taattcttgt tgaagataat ataaaatttt attcatccta
     4801 tctgcccata atttttagtg aggttttcaa gcaatctcaa agattggtac gagaaggtat
     4861 taatcttaca cacaagtttt taagaatgcg tgcacgccca aaaattatac tatgtacaac
     4921 atatgaggaa gcatggaatt attatgaaaa ataccaggaa tatgtacttg gattaatcct
     4981 tgatataaac ttcaaacatc acggtgtaaa agatcctgaa gccggaataa agtttgcgcg
     5041 tactgttcgt tctcagcacg atgatattcc aatcttgctg caatccagcg atgcgagatt
     5101 tagagagaaa gcttatgaaa tcggtgcttc atttatgcat aagaattctc caagattgct
     5161 gcaggagtta agagagttta tgcttcagaa ttttggcttt ggtgattttg tttttgttaa
     5221 taacgccgga caggaagttt ttagagcagc taatctatct gagttggaag aagcattaat
     5281 taaaattccg gatgaaagcc ttgtttatca tgcttcaaga aatcattttt caaaatggct
     5341 taaagcacgc actgaatttt atttgtcgct aaaattaagg ccgcataaag taagtgattt
     5401 cggttcagtt cagggattac gggatagatt gcttacttca cttcaggagt atagaaaaat
     5461 tcagcaaagc ggaataattc tagattttaa caaaaatatg ttcgatccca aaaacagctt
     5521 tgcaaggatc ggcggaggtt caataggagg caaagcaaga ggtttaggat ttattaacac
     5581 acttattata aactatgacc ttaaaaataa atttgataat gtag
//
LOCUS       DASWBY010000170         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_269003, whole genome shotgun sequence.
ACCESSION   DASWBY010000170 DASWBY010000000
VERSION     DASWBY010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269003"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..675)
                     /gene="ruvA"
                     /locus_tag="VF870_03100"
     CDS             complement(97..675)
                     /gene="ruvA"
                     /locus_tag="VF870_03100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX9251198.1"
                     /translation="MIGYLTGKLISSKPTQIILDVNGVGYLVNISISTFEKISDKETV
                     SLFIHTSVKEDSITLFGFFTQSEKEMFELLISISGIGPKVSLGILSGISVDDLKDAIA
                     NGNVSRLIAIPGIGRKTAERVVLELRNKVDAIKADGSIKITSAKDEAISALATLGYQR
                     QLAEKVMRDLLSENANYSLEELIRKALAGLNK"
     gene            complement(725..1099)
                     /locus_tag="VF870_03105"
     CDS             complement(725..1099)
                     /locus_tag="VF870_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9251199.1"
                     /translation="MKVISFEDLQVWKDSRILVKSIYQLTSDGKFSKDFGLREQIQRA
                     SVSIMNNIAEGFERNNNKEYIKFLGYSKGSAGEVRSMLYVATDLGYISQDSFNTLYQM
                     SINIITQLSNFIKYLKSSSIKK"
     gene            complement(1170..>1549)
                     /gene="ruvC"
                     /locus_tag="VF870_03110"
     CDS             complement(1170..>1549)
                     /gene="ruvC"
                     /locus_tag="VF870_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9251200.1"
                     /translation="IIKKYKPDEFAIETAFYGKNVQSAMKIGYARGVSILCALHNNVP
                     ASEYSPREVKKSVVGKGAASKDQVSFMIKAILNLKSEKIKVDESDALAIALCHAFRMK
                     SPVKKTKDWKSFIEAYPERVLNN"
BASE COUNT          502 a          271 c          215 g          561 t
ORIGIN      
        1 gcgaggagtc ttcgacgaag caatttacca cagatttgaa ataatgatta agattgcttc
       61 accttaatcg ggattcgcaa tgacatacag agagagttat ttattcaatc cagccagagc
      121 ttttcttata agttcttcta aagaatagtt agcattttct gaaagtaaat ctcgcataac
      181 tttttctgca agttgtcttt gataaccaag tgttgccaat gcagagatcg cttcatcttt
      241 tgcagatgtt attttaattg atccatcggc tttaattgca tcaactttgt ttcttaactc
      301 cagcacaaca cgctcagctg tttttctacc aataccggga atagcaatta atcttgaaac
      361 atttccattg gcaattgcat cctttaaatc atcaacagaa attccggata aaatacctaa
      421 acttactttt ggaccaattc cactaataga aattaacagc tcaaacattt ctttttcaga
      481 ttgagtgaaa aatccaaaca aagtaattga atcttcctta acacttgtgt gaatgaatag
      541 ggaaacagtt tctttgtcag aaatcttttc aaaagtgctg atagaaatgt taaccaaata
      601 accaacaccg tttacatcaa gaataatttg agttggtttg gatgaaataa gttttcctgt
      661 tagatatcct atcatagttt tttaaaatta aaagattgaa atattcaaag attcaaaaat
      721 tattttattt ttttatagaa cttgatttta agtatttaat aaagttagaa agttgagtaa
      781 taatattaat tgacatttga taaagtgtgt taaaactatc ttgtgaaata tagcccaaat
      841 cagttgcaac ataaagcatc gatcttactt ctccagctga tccttttgaa tatcccaaaa
      901 actttatgta ctctttgtta ttgtttctct caaaaccctc agcaatgtta ttcataattg
      961 aaactgaggc tctttgaatt tgttctctta gtccgaaatc tttactgaat tttccatcag
     1021 aagttagttg atagatagat ttaaccagaa ttcgactgtc tttccaaact tgtaaatctt
     1081 caaatgaaat caccttcatt taatcctcca aaagcatttg aatatttgtt tgaattttaa
     1141 tctttgaatt ttttaatctt tcaaattttt caattattaa gaactctttc cggataagct
     1201 tcaataaaag atttccaatc ctttgttttc tttacaggag atttcattct gaaagcatga
     1261 caaagtgcaa ttgcaagcgc atcgctttca tcaaccttaa ttttctctga ttttaagttt
     1321 agaattgcct taatcataaa cgaaacctga tctttacttg ctgcaccttt acccacaaca
     1381 gattttttaa cttctcttgg tgagtattca cttgcaggaa cattgttatg aagggcacaa
     1441 agaattgaaa cgccgcgtgc ataaccaatc ttcattgctg attgaacatt ttttccataa
     1501 aaagccgttt cgattgcaaa ttcatcaggc ttatattttt ttatgattt
//
LOCUS       DASWBY010000171         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_494973, whole genome shotgun sequence.
ACCESSION   DASWBY010000171 DASWBY010000000
VERSION     DASWBY010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494973"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..694
                     /locus_tag="VF870_03115"
     CDS             233..694
                     /locus_tag="VF870_03115"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9251201.1"
                     /translation="MNSKMKITGAAPVIAGTTFIYFIITVIISSMVKPSFIITEKHYD
                     VLIILGIILIIPGTLMVIRCGRKLLKSFKKGLLMTDGLYKIFRNPMYAAYMLFIIPGI
                     CLLFNSWLVLTTILVNYILFSIFIKSEHQYLQEKFGSEYELYLKNVFIKFL"
     gene            1068..>1758
                     /locus_tag="VF870_03120"
     CDS             1068..>1758
                     /locus_tag="VF870_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251202.1"
                     /translation="MKSYLLISLVLFAFILILFEGCGKSPSPQLVSSSTPTKEQLEKS
                     NFVKFVSELKFLPEDQRKNHIQGFLVSYPNSPIIEDSSIAIFYWYGKANSVLINGDIQ
                     LSWTVPDTMNRISCGYEDFFYKIYSLPADSRIDYQLIVDDSTITDPRNPIVTPSGFGL
                     HSQCAMPLFITKTVRESRNNIQHGAIDTLWFKSRQTSILPRMLKIYKPANYDSLFSLP
                     TLYVNDGFKAIE"
BASE COUNT          561 a          274 c          262 g          661 t
ORIGIN      
        1 tttatgtggt gctgtttttt atacatagtt acatttattg atttttataa taacaaaata
       61 atcaatccat caattaaatc agttaaattt tgcataatat gattatattc tttcgtgatt
      121 cctcccaaaa tcagataatt aataactaat aaattcaatt gacttgaatt gttttttaaa
      181 ttatgttttt ctgaaatgaa aattcaaaac cattaataag gaatctgtac aaatgaactc
      241 aaagatgaaa attaccggcg ctgcccctgt tattgcagga acaacattta tttattttat
      301 aataaccgta ataataagct cgatggttaa accttcattc attattactg aaaagcatta
      361 tgacgtatta attattttag gaattatatt aattattccg ggtactttaa tggtgatccg
      421 ttgtgggcgg aaattactaa aatcatttaa gaagggtttg ttaatgaccg atggattata
      481 taaaattttt agaaatccaa tgtatgccgc gtatatgctt tttattattc cgggtatctg
      541 tcttcttttt aattcctggt tagtgcttac aaccattctg gtaaactata ttcttttctc
      601 aatatttatc aaaagcgaac atcaatactt acaggaaaag ttcggcagtg aatatgaatt
      661 atatcttaaa aatgttttta tcaaattttt ataggccagc caataagctt tagttttagc
      721 ttttaattgg attatgtctt aactaaacga ttaagacata atctcttctt ttatttttat
      781 ccatttgcca ttttctatta ctgattctgc aaccgatgag attaaccggc tttgtctgcg
      841 atattgttta ctgtttttaa ttctaatcta tttgtatgct caacaaaatc tcttcagaag
      901 gttcttcgta aataattccg agagtggggt ggtacacatt tttattgatg agtggtttta
      961 tagttttgtc aatagttttg gttttgatgt tctgcacaaa ttacctttct tggtcctggc
     1021 agacttatat ttaaacaata aaaattttta tgttttgtga gcatcaaatg aaatcttatc
     1081 tacttataag cctagtctta ttcgctttta ttttaatttt atttgaagga tgcggcaaat
     1141 cgccttctcc acaattagtt tcttcatcaa ctccgacaaa agagcagctt gaaaaaagca
     1201 attttgtaaa atttgtttcc gaattaaaat tccttccgga agatcagaga aaaaatcaca
     1261 tacagggttt tttagttagt tatccaaact ctccaatcat cgaagacagc tcaatagcaa
     1321 ttttttactg gtatggaaaa gctaattctg tattgataaa tggtgatatt caattaagtt
     1381 ggacagttcc ggatacaatg aatcgaattt cttgtggata tgaagatttc ttttataaaa
     1441 tctattcttt gcctgcggat tcaagaattg attaccaatt aattgttgat gacagcacaa
     1501 ttacggatcc acgtaaccca attgtaactc caagcggatt tggtcttcac tcacaatgtg
     1561 ctatgccttt gtttataaca aaaactgtta gagaatctag gaacaacatt caacacggag
     1621 cgattgatac tctttggttt aaaagccgcc agacatcaat actgcctaga atgttaaaaa
     1681 tttataaacc agctaattat gattctcttt tttctcttcc tacactttac gttaatgatg
     1741 gattcaaggc aatagaat
//
LOCUS       DASWBY010000172         3698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_8539, whole genome shotgun sequence.
ACCESSION   DASWBY010000172 DASWBY010000000
VERSION     DASWBY010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8539"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VF870_03125"
     CDS             complement(<1..1230)
                     /locus_tag="VF870_03125"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX9251203.1"
                     /translation="MSEKKNKPADAVANIVSLAKRRGFVFQSSEIYGGLNGCWDYGPL
                     GVELLKNIKEDWWKFMTYRDNVEGLDASILMHPKVWEASGHVENFTDPMIDCKQCKAR
                     FRLDVLGDSINEKKKEKALAELKNDFKDDASILSKIEDKLKNPGDDDPFNLLLEEEQV
                     GKTLLSKLNCPQCGNANTFTDARKFNLMFKTFVGPVEDSGSVVYLRPETAQGIFVNFL
                     NVQSSARQKLPFGIAQIGKAFRNEINTKNFLFRTREFEQMEMQFFVKPDDAKEWYEYW
                     KAARIEWFKSLGMTAAKLRYHDHIKLAHYAAAATDIEYEFPFGWGEIEGIHNRTNFDL
                     SRHEEFSGKSLKYFDEEAKEKYVPFIIETSAGASRSFMAFLIDAYYEEEVNGEVRSVL
                     RFHPRLAPIKAAIFPLVN"
     gene            1389..1997
                     /locus_tag="VF870_03130"
     CDS             1389..1997
                     /locus_tag="VF870_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX9251204.1"
                     /translation="MFKKLLPKEEKYFEDFKEMITFIEEMAVHTEKIFSFDDPQTHIL
                     KMKPLEVRCDEIAGKITKRLNKTYITPFDREDIFALIKRLDDISDMLLGATIRVDTFH
                     IEKKIEHTDKLALIIREQVKELGTAIQDLKVKRVNELKAVKDLEVEADKVYQQAIKEL
                     FENEKDAIELIKKKEIIDLLERTSDRCQSTANVILSIFLKNT"
     gene            2007..2996
                     /locus_tag="VF870_03135"
     CDS             2007..2996
                     /locus_tag="VF870_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX9251205.1"
                     /translation="MEIVVLIIALALVFDFYNGMNDAANSIATVVSTRVLTPFQAVAW
                     AAFFNFAAAFIFGVHVATTIGTGIVDVKIVDNFVVLSGLVGAIILTASATHFGMPISV
                     SHAIIGGFGGAAVMKAGFGALIISGYTKVLIFIFLAPILGMLMALLFSILTIWIVKDM
                     NPRKVDKQFRRLQLLSAALYSLSHGSNDAQKTIGIITIALFTNGVLGSTFYVPTWVIF
                     LSYSFIALGTLLGGWKVIKTLGMRVTKLTPFGGFSAETSAGLTIIWATLLGVPVSTTH
                     TITGSIIGVGSVKGFSAVRWGVAKNILWAWVLTIPLSALFAMITYLIATGIFS"
     gene            complement(2993..>3698)
                     /gene="amrB"
                     /locus_tag="VF870_03140"
     CDS             complement(2993..>3698)
                     /gene="amrB"
                     /locus_tag="VF870_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX9251206.1"
                     /translation="KIFGIVSPHAGYIYSGKTAAYAYNLLKDKEYKTVIIISPSHAEY
                     FPGISIYDGDAYETPLGIVEIDNEMTDRLIENSKIIFRGRQGHRKEHALEVQIPFLQS
                     VLKNFKIVPIVMGDQGKMFVDELAEKISKVVDDETLVVASSDMSHFYSSEEADRLDSV
                     VEERINDFDFENLLIDLDYHKCEACGGGPIVAMMKAASKKNINKAVVINRSDSGDVTG
                     DKSEVVGYLSAVVYNN"
BASE COUNT         1178 a          674 c          611 g         1235 t
ORIGIN      
        1 attaaccaat gggaaaattg ctgctttaat tggggcaagt cttggatgaa atcttaaaac
       61 tgatctcact tcaccattta cttcttcttc ataataagca tcaattaaaa atgccataaa
      121 actgcggctg gctccggcag acgtttcaat tatgaacggc acatattttt cctttgcttc
      181 ctcatcaaaa tactttaaag atttgccgga aaattcttcg tgtctgctta aatcaaaatt
      241 agttctgttg tgtattcctt caatctcacc ccagccaaaa ggaaattcat attcgatatc
      301 agttgcggcg gcggcgtagt gcgcaagttt tatatgatcg tgataccgaa gttttgctgc
      361 tgtcattcca agtgatttaa accattcaat tcttgcagct ttccagtatt cgtaccactc
      421 ttttgcatca tcgggcttaa caaaaaactg catttccatc tgttcaaact cgcgtgtacg
      481 gaaaaggaaa ttttttgtgt tgatttcatt tctaaatgct tttccaatct gcgcaatacc
      541 aaaaggtagt ttttgtcttg cagaactttg tacatttaag aagttaacaa aaattccctg
      601 agcagtttcc ggtcttaagt aaacaacact tccgctgtct tcaacaggac caacaaatgt
      661 tttaaacatc aaattaaact tacgtgcatc ggtaaatgta tttgcatttc cgcactgtgg
      721 acaatttaat tttgataaaa gagttttacc aacttgttcc tcttccaata aaagattaaa
      781 tggatcatca tcaccaggat tcttaagctt atcttcaatt ttgcttaaga tagaagcatc
      841 atctttaaaa tcatttttta attccgctaa tgctttctct tttttctttt cgttgattga
      901 atcaccaaga acatcaagac gaaaacgagc tttacactgt ttacaatcta tcattggatc
      961 agtaaaattc tcaacgtgac ccgaagcttc ccaaaccttt ggatgcataa gaatggaagc
     1021 atcaagacct tccacattat cgcggtaagt cataaacttc caccagtcct ctttaatatt
     1081 ttttagaagt tcaactccca atggaccata atcccagcat ccgtttaatc cgccataaat
     1141 ttcactggat tgaaacacaa aacctcttct tttagcaaga gaaacaatat ttgcaacagc
     1201 atctgcaggt ttatttttct tttcgctcac atttcacctt aattttaatc gataatttga
     1261 ggtgtgaata tagtaaaacc aaaccaatat tttgaatata atttattggg gaattactgc
     1321 cgtttactta gttttggatt gtcgaaggca ctctttttaa aaatcatttc aaaaataatt
     1381 ggtgttttat gtttaaaaaa ttattaccta aagaagaaaa gtattttgaa gactttaaag
     1441 agatgataac tttcatcgaa gaaatggcgg ttcatacaga aaaaatattt tcattcgatg
     1501 atccgcaaac ccacatccta aaaatgaaac ctttggaagt tagatgtgat gaaattgcag
     1561 gtaagatcac taaaagatta aataaaacat atataactcc ttttgacaga gaggatattt
     1621 ttgctctgat aaaaagatta gatgatatta gtgatatgct tttaggtgct accatcagag
     1681 ttgatacttt tcatatagaa aaaaaaatag aacacacaga caaacttgct ttaatcatcc
     1741 gtgaacaggt aaaagaactc ggtactgcta ttcaggattt aaaagtaaaa agagttaatg
     1801 aattaaaagc tgtgaaagat cttgaagttg aagcagataa agtttaccag caggcaatta
     1861 aagaactatt cgaaaatgaa aaagacgcaa tcgaattgat taagaaaaaa gaaataatag
     1921 atttgctcga acgaaccagt gaccgctgcc aatcaacagc caatgtaatt ttatcaatat
     1981 tccttaagaa cacataaaaa taaattatgg aaattgttgt actgataata gcgcttgcac
     2041 ttgtttttga tttctataat ggaatgaatg atgctgcaaa ttcaattgct actgttgtat
     2101 caacacgggt gcttacacca tttcaggctg ttgcctgggc agcttttttc aattttgccg
     2161 ctgcatttat ttttggagtt cacgttgcaa caacaattgg cactggtatc gttgatgtaa
     2221 aaattgtcga taactttgtt gtcctttccg gtttagttgg cgcaattatt ttaacagcat
     2281 cagccacaca ttttggtatg cctataagcg tttcacacgc aattattgga ggttttggtg
     2341 gtgctgctgt aatgaaagca ggatttggag cattaattat cagtggttat acaaaagtat
     2401 taatatttat cttccttgca ccaatactcg gaatgttgat ggctctattg ttctcaatcc
     2461 ttacaatatg gatagtcaaa gatatgaatc cacgcaaagt ggataaacaa tttagaagat
     2521 tacaacttct ctccgctgca ctttacagtt taagtcatgg atcaaatgat gcacaaaaga
     2581 caattggaat tattacaatt gctttattta caaatggagt tttgggatcc acgttttatg
     2641 tgccgacctg ggttatattt ttaagttatt catttatcgc attgggtaca ttacttggag
     2701 gatggaaggt tatcaaaaca cttgggatgc gtgttactaa actaacaccg ttcggtggtt
     2761 ttagtgcaga aacttctgca gggttaacaa ttatctgggc aaccttatta ggagttcctg
     2821 ttagcactac tcacactata acagggtcaa taattggagt gggatcagtt aaaggatttt
     2881 ctgctgtgcg ttggggtgtt gctaaaaata ttttgtgggc gtgggtgctt actatccccc
     2941 tctcagcttt gtttgcaatg attacttatt taattgcaac aggaattttt agttaattat
     3001 tgtaaacaac tgcagataaa tatccaacaa cttcagattt atctccggtt acatcaccgg
     3061 aatcacttcg atttattaca acagccttat taatattttt ttttgatgct gctttcatca
     3121 ttgcaactat tggtccgccg ccgcatgctt cacatttgtg ataatcaaga tctataagaa
     3181 gattttcaaa atcaaaatca tttatccttt cttcaacaac tgaatcaagt cgatctgctt
     3241 cttcagaaga ataaaaatgc gacatatctg aacttgcaac aactaaagtt tcatcatcaa
     3301 ctactttaga aattttttct gcaagttcat caacaaacat cttgccttga tcgcccatta
     3361 caataggaac aatcttaaaa ttctttaaca ctgattgcaa aaatggaatc tgaacctcaa
     3421 gagcatgttc ttttctatgt ccttgccggc ctctaaaaat tattttactg ttttcgatta
     3481 atctatcagt catttcgtta tcaatttcaa caatccctaa aggagtttcg taagcgtcgc
     3541 catcatatat agagattcct ggaaaatatt ctgcgtgact aggagaaatt ataattacag
     3601 ttttatactc cttatctttt aaaagattat aggcgtatgc tgcggttttt cctgaataaa
     3661 tatatccagc atgcggagag acaatcccaa atatttta
//
LOCUS       DASWBY010000173         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_81405, whole genome shotgun sequence.
ACCESSION   DASWBY010000173 DASWBY010000000
VERSION     DASWBY010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81405"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(431..1171)
                     /locus_tag="VF870_03145"
     CDS             complement(431..1171)
                     /locus_tag="VF870_03145"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX9251207.1"
                     /translation="MITCKNCNNDFEGKYCNDCGQSADTERLDFIFLIKNLHKNFIKY
                     FHKGIFYTSRQMFTRPGHSIREYIEGKRVQHFEPIGLLLTFAAFYAVLYHTFDIYMFS
                     GLSNNALDGKLDFKSVSNWMTDHFAMVTLLFVPVYSLGSYLSFRKQEYNFYEHIYLNT
                     FLASQRLLIRIATFPLLVVLNRTENIFILKDLYILADIVLMVWSYTQFFNKVKRIKAV
                     LLSLLSYSIFFVCLLIITAVLLEIFRLI"
     gene            complement(1214..>2124)
                     /locus_tag="VF870_03150"
     CDS             complement(1214..>2124)
                     /locus_tag="VF870_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251208.1"
                     /translation="FMPAIKTGTKFILSLQPIFMKTPIPGLSQGLGEFPRFRAELGAF
                     VGFSPSMRGWLFSGGFGNGQETIGVSGGLEVAARLGIGLDGVLNESGDGLAFLDVGWR
                     QDGSSSTEVLDVEQLRAYGNVYSTIPGRSAFSARLRIPFYTLPGDLLILGPLLFFIDQ
                     EALTSVGVSAVNGGLIPWQAGIETSFGRFQFVLGREIAVYLYGRTKTPDALVTYSTGE
                     NGVEDLFVISYRSTTVEFPIVEYRPFRSFDADQRSSLFIQLYGAADFPHNVQVLESLS
                     GTTIPPDLKTVWHFGLRLIFDWRHYF"
BASE COUNT          718 a          418 c          365 g          623 t
ORIGIN      
        1 gccgtcattt ccgaaagaaa catcagcatg tattctgctg aactggccga gtccaaaaac
       61 tccgtctacg gcatagtgtc cgccccaggc atcaccaaaa cggataccaa cttcctgggc
      121 ttgtgcgaac tgagagatac caataaatat tactgctgca agaagaaatt ttttcatttt
      181 ttcccttatt tattttatga ttatgtgatt agttatacta ttagagtaat agaatttaaa
      241 gctgcagaca aagctaataa aaagacttgt cttttgtaat tattgatacc ttattaaatg
      301 ggaaaaatat ctttattcct ttcccccggc agcaaattta tgttaaatct ttccccttgg
      361 aagagtgaaa attgacggtt ttctgctggt aatcatcagt gagagaaaag aagtgaagtt
      421 agctatatcg ctatatcagg cgaaaaattt ccagcaatac tgccgtgata ataagtaagc
      481 aaacaaaaaa tattgaatag cttaataggc tgagaaggac tgctttaatt cttttaactt
      541 tattaaaaaa ttgtgtgtag ctccagacca ttaatacaat atctgccagt atataaaggt
      601 ctttgagaat aaaaatgttt tctgttctat ttaaaactac tagcagcgga aatgttgcaa
      661 tcctgattag cagcctttgg ctggctaaaa aagtatttag gtaaatatgt tcataaaaat
      721 tatactcttg tttcctgaac gacaaatacg aacctaaaga ataaaccgga acgaaaagta
      781 aagttaccat agcaaaatga tcagtcatcc aattgctcac gcttttaaag tctaactttc
      841 catcaagagc attatttgaa agtcctgaaa acatataaat gtcaaaggta tgatatagca
      901 cggcgtaaaa agcggcaaat gttaataata gacctatcgg ttcaaaatgc tgtacacgct
      961 tgccttcaat gtattcccgg attgaatgtc cgggtcttgt aaacatctgc cgggaagtat
     1021 agaaaattcc tttatgaaaa tatttaataa aatttttgtg cagatttttt ataagaaata
     1081 taaaatccag tcgttctgta tctgcgcttt gtccgcagtc attacaatac ttcccttcaa
     1141 aatcattatt gcagttttta caggtaatca tatttttgta aaattaattt aaagttgata
     1201 atagttttta tgtctagaaa taatgtctcc aatcaaaaat caatcttaaa ccaaagtgcc
     1261 agactgtttt tagatccggt ggtattgttg ttcctgaaag cgattcaagg acttgaacat
     1321 tatgaggaaa atcagcagca ccgtatagct gaataaaaag cgatgatctc tgatctgcat
     1381 cgaatgatct aaacggtctg tattctacaa tcggaaactc aaccgtagta gagcggtaag
     1441 aaataacaaa caaatcttca acgccgtttt caccagtaga ataagtaact agtgcatccg
     1501 gtgtttttgt tctgccatac aggtaaaccg caatttctct cccaagaaca aattggaaac
     1561 gaccaaaaga agtttcgatt cctgcctgcc acggaataag cccgccatta actgcagaga
     1621 ctccaacact ggttaatgct tcctgatcaa taaaaaataa taatggacca agaattaaaa
     1681 gatcacccgg taatgtataa aatggaattc ttaatctggc actgaaagca gatcttccgg
     1741 gaattgttga atatacattt ccatatgctc tgagctgttc aacatccagc acttctgtgg
     1801 atgaagaacc atcctgtctc caaccaacat caagaaaagc cagaccgtct cccgattcat
     1861 ttaatacacc gtctaaccca attcctaaac gcgccgctac ctccagtcca cctgaaacac
     1921 cgatagtttc ttgtccgttt ccaaaacctc cggaaaataa ccagccgcgc attgagggtg
     1981 aaaatccaac aaaagcaccc agctcggctc taaatctggg aaactccccc aagccttgtg
     2041 ataaaccggg aattggtgtt ttcataaata ttggttgcaa tgacaaaata aatttagtgc
     2101 ctgtttttat tgcaggcata aaat
//
LOCUS       DASWBY010000174         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_571795, whole genome shotgun sequence.
ACCESSION   DASWBY010000174 DASWBY010000000
VERSION     DASWBY010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571795"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VF870_03155"
     CDS             <1..482
                     /locus_tag="VF870_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9251209.1"
                     /translation="GVSVHQIKKWNNIFGDQIAAGRTLKIYNNTTSSSYGDNTTKNSA
                     NVNYYKIKSGDSIGSIAEKYGVKISDIQKWNNISGNKILAGSTLKIYSDANVNDIENV
                     TVKTNKNSKSNQQSYTVKSGDSLYSIASKNNTTVAKLKSLNNLTSNKIKAGQKIRLN"
     regulatory      494..597
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            648..>1622
                     /locus_tag="VF870_03160"
     CDS             648..>1622
                     /locus_tag="VF870_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX9251210.1"
                     /translation="MKKTIILFLLITFQILPQSRTLNGIVKDSETKSFLQFANVLIKG
                     SNIGTTTNDQGRFTLSGKFNESDSLLVSYVGYKSSITPLSELNSDLLVIELQRISLLS
                     QTILIEASVGKKGFTPMSFDKIAKEEIEKDYIVQDIPNYLSQLPSTTFYSENGNGIGY
                     NYLSIRGFDQRRISVSINGIPQNDPEDHNVYWLDFPDLLASTELIQVQRGAGSGSVGY
                     PAIGGSINIITSPFSEKPQINLSAAYGSFNTRKYSAAFSSGLINNKYSIYAKLSQILS
                     GGYREKSWSKLNSYHLSAARYDDKLTSQLNLFGGPISDGLAYTGIAKFA"
BASE COUNT          575 a          270 c          271 g          506 t
ORIGIN      
        1 ttggagtatc tgttcatcaa ataaaaaaat ggaataatat tttcggtgat caaatagccg
       61 ccggcagaac cttaaaaatt tacaataata caacgagttc atcatatggt gataacacaa
      121 ctaagaactc agcaaatgta aattactaca aaattaaatc aggtgattcc ataggcagta
      181 ttgctgaaaa atacggagta aaaatttctg atattcaaaa atggaataat atttccggca
      241 acaaaattct ggctggcagt actttaaaaa tttattctga tgcaaatgta aatgatattg
      301 agaacgtaac tgtaaagaca aataaaaatt caaaatcgaa tcagcaaagt tatactgtaa
      361 aaagcggtga ttccctctat tcaatagcat caaagaataa cacaactgtt gctaaattaa
      421 aatcactaaa taatcttact agcaacaaaa ttaaagcagg gcaaaagatt agacttaatt
      481 aattttttat ttactgagcc gttaggggtg ttatcccgct acccgtggga tatctgagat
      541 aataccctaa aacctgatct gggtaattcc agcgtaggaa aacgggctaa gaatgatttt
      601 ttttcaatag ccgtttctcc aacggctatt tttttaggag taatattatg aaaaaaacaa
      661 tcattctttt tttattaata acatttcaaa tcctacctca atcaagaaca ttaaacggaa
      721 ttgttaaaga ctccgaaaca aagtcctttt tacaattcgc aaatgtttta ataaaaggtt
      781 caaatatcgg aactacaaca aatgatcagg gaagatttac actctcagga aaatttaatg
      841 agtctgattc attattagtc agttatgttg gatataaaag ttctataact ccgttatcag
      901 aattaaattc tgatttatta gttattgaac ttcagagaat atcattactt tctcaaacta
      961 ttttaatcga agcaagtgtg ggcaaaaaag gttttacccc aatgtctttt gataaaattg
     1021 ctaaagaaga gatagaaaaa gattacatcg ttcaggatat cccgaattat ttatcacaat
     1081 taccttcaac aactttctat tcagagaatg gaaatgggat tgggtacaat tatttaagta
     1141 ttcgcggttt tgatcagcga agaatttcag tttctattaa tggtattcca caaaatgatc
     1201 cagaagacca taacgtttat tggttggatt ttcctgatct gctcgccagc acagaactta
     1261 ttcaggttca acggggcgca ggttctggca gtgtcggata tccggcaatt ggcggatcaa
     1321 ttaatattat tacctctcca ttttcagaaa aaccgcaaat taatctttcg gctgcatacg
     1381 gaagttttaa tacgagaaaa tattctgctg ctttttcaag cggacttata aataacaagt
     1441 attccatcta tgcaaagctt tctcaaattc ttagtggtgg gtacagggaa aaatcctggt
     1501 caaaactaaa ttcatatcat ctttctgctg cgcggtatga tgataagtta acgtcgcaat
     1561 taaatctttt tggcggacca atttctgacg gtcttgcata taccggaata gccaagtttg
     1621 ct
//
LOCUS       DASWBY010000175         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_81450, whole genome shotgun sequence.
ACCESSION   DASWBY010000175 DASWBY010000000
VERSION     DASWBY010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81450"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VF870_03165"
     CDS             complement(<1..521)
                     /locus_tag="VF870_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEX9251211.1"
                     /translation="MKKNVLVILFLISSNLLFASGNKNLEKAIRLIKTDIEIVIDGKI
                     DEAWNYADSTVNFFQLDPYYNQPTSVKTVAKVLTTEEALYCLMICYDNKKYIQANSGM
                     QDWYTGDIASIMLDTFGDKQTAYKFAVNASGVKSDSRMLDDARNRDYSWDGIWQGTSK
                     IYDWGYVVEMKIP"
     gene            complement(805..1665)
                     /locus_tag="VF870_03170"
     CDS             complement(805..1665)
                     /locus_tag="VF870_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9251212.1"
                     /translation="MKITKQFTNRESKSLDQYLQEIGKVDLLTPDDEIELAIKIKKGD
                     RLAQERLIKANLRFVVSVAKQFQNQGLSLGDLINEGNIGLIKAAERFDETRGFKFISY
                     AVWWVRQSIMQAIADQSRVVRLPLNRVGNLTKISKAYRDLEQEYERKPTTDELAQILE
                     MTSEEVAYALQIAGRQVSMDAPLKEGDDGKNSLVDILPNDEQPLPDNKLMTESLKKEV
                     ANVLSTLSEREAEVIKLYFGIDGDHSATLEEIGERFNLTRERVRQIKEKALRNLRQSK
                     RSGRLKAYLG"
     gene            complement(1808..3208)
                     /locus_tag="VF870_03175"
     CDS             complement(1808..3208)
                     /locus_tag="VF870_03175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9251213.1"
                     /translation="MQLEFIGGARTVTGSQHLLHINGKKILLECGLFQGRRSDTYDKN
                     KNFRFNPAEIDALILSHAHIDHSGNIPNLVSKGFNGLIYATSATVDLCQIMLRDSAHL
                     QEKDIEWVNKKKAKKGEEPFESLYSLDDVEKAMQHFVGVQYNRTIDLYPGIKFMFRDA
                     GHILGSAGVRLEIEEEGGKKISLGFSGDIGRPESPVIKSPDVLRDIDVLIMESTYGNR
                     LHSPAEEVEEDFVSTINPVINRGGKIIIPAFAVGRTQTIVYMLHKLFDENRIPEIPIY
                     VDSPLAVEATNIFRSHPECLDRETYRIFLQNGEDPFGFGRLKYIKKTEESKQLNDKPG
                     PMIIISASGMAEGGRILHHLVNNISNPKNLILFVGYAAEHTLARRIMNGNEKVNIFGE
                     EFEVKAQIKKMDYFSGHADQKELLDYLRLNDQNMLKNIFLVHGEEEMALPFKEKILQE
                     GYKNVQFPASGEIINI"
BASE COUNT          947 a          645 c          508 g         1133 t
ORIGIN      
        1 tatggaatct tcatttcaac aacataaccc caatcatata tcttagaagt accttgccat
       61 attccatccc agctataatc gcggttacgc gcatcatcta gcattcttga atcagactta
      121 actccggaag cattaacagc aaatttataa gcagtttgtt tatcaccaaa cgtatccaac
      181 attattgagg caatatcgcc ggtgtaccaa tcctgcattc ccgagtttgc ttgaatatac
      241 tttttattgt cataacaaat catcaaacaa taaagagctt cttcggttgt taaaacctta
      301 gctacggttt ttacagaagt tggttgattg tagtaaggat ctaattgaaa aaaattaacc
      361 gtcgaatcgg cataattcca tgcctcgtca atctttccgt caattactat ttcaatatcc
      421 gttttgatta gccgaatcgc tttctccagg tttttattcc ctgaagcaaa aagcaaatta
      481 gaagaaatta gaaataaaat taccagtacg ttttttttca taaaccaatg tcatttttca
      541 tttagatctt tttctgcatc cgaaagttac tttgtataaa gagaatatgt tgatgtttta
      601 gataagcggt taaatgaatg cataaacgac aaaaaggctt gttgtgtttt tttgtttgta
      661 tgttttaaat gaagtgtttt ttaggcaggt tcacgcaaag gttgcaaagt ttttcgcgaa
      721 gctcgctaag tagtccagtc aaaatggttt aaaaaacaaa agccccggta aatcggggct
      781 ttaaaacctg aaacgaatta ttttttatcc taaataagct ttcagtcttc cacttctttt
      841 tgattgcctt aaatttctta aagctttttc tttaatctgt ctgactcgtt cacgagtgag
      901 attaaatctt tcgccaatct cctctaaagt agctgaatgg tctccatcaa ttccgaagta
      961 taattttatt acttctgctt ctctttctga gagagttgag agaacatttg caacttcttt
     1021 tttcaaagac tcggtcatca atttattatc cggcaaaggc tgttcatcat taggaagaat
     1081 atcaactaaa gaattttttc cgtcatcacc ttcctttaaa ggtgcatcca ttgacacttg
     1141 tcttccagca atctgaagcg cataagcaac ttcttcagaa gtcatttcaa gaatctgagc
     1201 aagctcatca gttgtgggct ttctttcata ttcctgttca agatctctgt aagctttgct
     1261 aatcttggta agatttccaa ctctgttaag cggtaatctt acaactcttg attgatctgc
     1321 aattgcctgc ataatagatt gtctaaccca ccacacagcg taagagatga atttgaatcc
     1381 acgggtttca tcaaaccttt cagcggcttt tatcaagcca atatttcctt cattgatgag
     1441 atcgccgaga gataagcctt ggttttgaaa ttgttttgca acgctcacta caaatcggag
     1501 gtttgctttt attaatcttt cctgggctaa tctatctccc tttttaattt tgatggcgag
     1561 ttcaatctca tcatcgggag tcagaagatc aactttaccg atttcttgta aatactggtc
     1621 taaagattta ctttctcggt tagtaaattg ctttgtgatc ttcaagcttt atatctcctt
     1681 aattgtgatt ataattattc gatattccat aatttgattc ttcttgacgg aagaaccgga
     1741 gtgctgattt tgtaaatgac aaatataaca cttaatcgga taaaagtaag ataaaaatct
     1801 atcctgacta aatattaatt atttcgcccg aagccggaaa ttgtacgttt ttgtagcctt
     1861 cctgaagtat tttttcctta aaaggcagcg ccatttcttc ttcaccatgc actaaaaata
     1921 tgttttttaa catgttttga tcatttagac gcaggtaatc caataattcc ttctgatcag
     1981 cgtgaccgga gaagtaatcc attttcttga tttgggcctt aacttcaaat tcctcaccaa
     2041 aaatgttaac cttttcattt ccattcataa tccttctggc aagggtgtgt tctgcagcat
     2101 atccaacaaa taaaattaaa tttttaggat tactaatatt atttacgaga tgatgaagta
     2161 ttcttccgcc ttccgccatt ccggatgctg agataataat cattgggccg ggtttgtcat
     2221 taagctgttt tgattcttct gttttcttaa tgtacttcag ccttccaaaa ccgaatgggt
     2281 cttctccatt ctgtaaaaag atacgatatg tttctctatc aaggcactct gggtgcgaac
     2341 gaaaaatatt tgtggcttcc accgctaaag gagaatccac gtaaatcgga atttcaggaa
     2401 ttctgttttc atcaaaaagt ttatgaagca tgtaaacaat agtctgagtt cttccaactg
     2461 caaatgccgg aataataatc tttccgcctc tgttaataac cggatttatt gttgatacaa
     2521 aatcttcttc aacttcttct gccggcgagt gtaatctgtt tccatatgtt gattccataa
     2581 ttaacacatc aatatcccgc aaaacatccg gagattttat taccggggac tctggtcttc
     2641 caatatctcc tgaaaatcct aaactaattt ttttaccacc ttcttcttca atttctaatc
     2701 gcacacctgc agaaccaaga atatgccctg catctctaaa cataaatttt attcctggat
     2761 acaaatcaat tgttctgttg tattgaactc caacaaaatg ctgcattgct ttttctacat
     2821 catctaaaga ataaagtgat tcgaatggtt cttcaccttt ttttgctttc ttcttattaa
     2881 cccattcaat atctttttcc tgaagatgag cagaatcacg aagcataatc tgacacagat
     2941 ctactgttgc agatgttgca tagattaatc cattaaaccc tttacttaca agatttggta
     3001 tgttgccgct gtgatctatg tgagcatgag ataaaattaa agcatcaatt tccgcaggat
     3061 taaatctgaa atttttattc ttatcataag tatcacttcg cctgccctga aacaatccgc
     3121 attcgagtaa aatctttttt ccgtttatgt gaagtaagtg ttgagatccc gttactgttc
     3181 tggctccgcc aataaattct aattgcaaag gatactcctt aaattattaa cag
//
LOCUS       DASWBY010000176         7695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_607924, whole genome shotgun sequence.
ACCESSION   DASWBY010000176 DASWBY010000000
VERSION     DASWBY010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607924"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VF870_03180"
     CDS             <1..617
                     /locus_tag="VF870_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX9251214.1"
                     /translation="TQFIESISIKEKQVIYVAGFPEGSQVDILAVDNQSDIALIGRKY
                     GSQKGIFFPTFNYPLGKAKEVEWGTFVYLVGYPINFKIITKAIVSSPRRDDAGSFLVD
                     AVVNPGFSGGLVLAIRDGVPNFELIGMVQWVPEEEESLIYPENPKTKSKYNPVVPYEG
                     NLFVRKHSSIRYGIAKIIPVENIMTFLLENKSYLNKQGFYIFQE"
     gene            678..896
                     /locus_tag="VF870_03185"
     CDS             678..896
                     /locus_tag="VF870_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251215.1"
                     /translation="MTKEIKQIEINTTCPICNKTISELWVARLDSVIGIRYAYICTNC
                     KNLIKITKEKYLDTSNLHQILFSQSTTT"
     gene            complement(926..1975)
                     /gene="mnmA"
                     /locus_tag="VF870_03190"
                     /gene_synonym="trmU"
     CDS             complement(926..1975)
                     /gene="mnmA"
                     /locus_tag="VF870_03190"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX9251216.1"
                     /translation="MKVAVLLSGGVDSSVALRLLKDEGHDVTAFYLKIWLQDEFSFLG
                     DCPWEEDLEFARAVCKQADVPLETINMQTEYWDSVVSYTISEIKEGRTPNPDMFCNRL
                     IKFGEFINKIDNSFEKVASGHYAKIEKVNDKFLLKTSPDPIKDQTYFLAYLTQQQLSR
                     ALFPIGSYKKKQIRELAHNYDLPNMTRKDSQGICFLGKIKFSDFIKHHLGELEGEIID
                     IDTNKVMGKHKGFYFYTIGQRSGLRLGGGPWYVISKDIEKNIVYISKENITNRARKEF
                     KVGKYNWITGDRPDDGNYLVKIRHGEHFYNCNLNFDGDTAKVVTEKEDRGIAPGQFAV
                     FYKDDVCLGGSIILE"
     gene            complement(1972..2478)
                     /locus_tag="VF870_03195"
     CDS             complement(1972..2478)
                     /locus_tag="VF870_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251217.1"
                     /translation="MQTFTYPLFWKFIYRYGNLVISPLLLLYTLSLVALIDKNLIILI
                     PFLLALFILYFLNKSYINFYKLVPYKIEIDDEKIICTDFLFRDKSITIFIKDIESISG
                     GIFDGRYRGLMKVCDGKNKLCVGFFDRINNSSKLVTLILSKVDKKIYDKVIEQIKSIK
                     IKPDIKSK"
     gene            complement(2604..3059)
                     /locus_tag="VF870_03200"
     CDS             complement(2604..3059)
                     /locus_tag="VF870_03200"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HEX9251218.1"
                     /translation="MNKIVILFFLLLSVAAYSQTDKYGKEISLKEKTSISKILAQPEE
                     YVGKTVLVEGKIVEVCAMAGCWMDLKSDAENQKIKIKVKDGDIVFPVEAKGKDALVEG
                     TVYKVELTKEEAVDYYKHVAEEQGTEFDGATVTGPVTIYQIKGIGAEIK"
     gene            complement(3095..3913)
                     /locus_tag="VF870_03205"
     CDS             complement(3095..3913)
                     /locus_tag="VF870_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251219.1"
                     /translation="MKHINYLFIGIIVSIFFTGCLQVNTTVNLNKDGSGTIEETVLMK
                     TDVINMMKEFAMSFDTTKSEEFNMFNEKDLKDKASNFGEGVKYVSGEKFATDNYEGFK
                     AVYSFSDINKIKLNPSPEDKIPYGAELGETEVESPGDLLKFNFKKGNPSTLVINFPKP
                     QPEDKIESEDTTEFQDTTLNEEAEQKMIEMFDGMKMALYFNFKDKIVETDASFVDGSK
                     VTFLDIDFSEIIKNKDVFKKFQSSKPETLEQFKEVVGDMKGIKIEFKDQVTIKF"
     gene            complement(3931..5295)
                     /locus_tag="VF870_03210"
     CDS             complement(3931..5295)
                     /locus_tag="VF870_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9251220.1"
                     /translation="MAVKKNPASNKKTPKTFVLDTNVILHDAASINMFQENDVIIPLA
                     VIEELDHFKRGSQVINLNAREFARTLDSMTGSALFNGGISMGKGRGKLRIAISKGLAP
                     AIREAFRDDTPDHRVLSVAYEWHEKLKGKHQVILVTKDVNLRMKAKALGVLAEDYTTD
                     RVKSIEELYSGKGVIENADDDLLVKLFQPPYQVSAKPFLKKLNGEALPNKYYILRNTN
                     RSVLAHLDQNKEFIIKVDKTNIYGITPRNAEQTFAVDALINPGIQLVSMTGKAGTGKT
                     LLALASALHVKRNYRQIFVARPIVPLSNKDIGYLPGDVESKLAPYMQPLWDNIKVIQD
                     QFPENDKQHQAIDNMIKEEKLVVEPLSYIRGRSLQRIFFIVDEAQNLTPHEIKTIITR
                     AGEGAKVVFTGDIYQIDHPYLDGQSNGLSYLIDRFKGQKLYAHINLEKGERSELAELA
                     SNLL"
     gene            complement(5462..7393)
                     /locus_tag="VF870_03215"
     CDS             complement(5462..7393)
                     /locus_tag="VF870_03215"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9251221.1"
                     /translation="MLVEIGYFLSSENPNEAIKYLDEAIKLADDTKNRWSKADAVFNK
                     GVALWHLGNIDESGKYYDDAIKIYKELNDSLSLIKVFNSQAINYQMKGNVDLAFETFL
                     RSLDYAKKMGDNATVFNTLLNIGIMYDNNSNYDKCLQYYNEALKYSDYTDKSSLALLQ
                     SYIAEVYLTLKNNEKAEEYLKQALENSKFSNDTNSLIWAYSSLGRIELDKKNFAKAEN
                     YFKESLTLAKQSDFKLEIIHSLTDLGKFYSTTNSLNKAESYLIEALRLSEEMNSLSDQ
                     NVVYGELASLYYKKQDYKKAYDYHQKYKVSNDSLFVLSNSEKLLEIQAEHELKQKERE
                     TELLLNENVLQKKIIISQRIIALVIALLAIASIIFIWVLLRNRNKILKAKNLLQDKTE
                     EIESNRIEISKNNEVLVGLNATKDKFFSIIAHDLRNPIAAFVNISELLESEYDRMSDN
                     DKKEIISQMNSSSKNLIMLLENLLTWARLSNNKIDIYTERLFIKDVLESSIFPYLQSA
                     QNKKIKINVDVPVALLIKTDRFIIQTIIGNLINNAIKFSNQHSEINISVLNSNGMYRL
                     VIKDHGIGIEVSQLRNIFVLGKASTGTGTMGEGGTGLGLVLVKELAEKLNWHIEVKSK
                     INSGTEFILTIPKTDIELS"
BASE COUNT         2469 a         1366 c         1085 g         2775 t
ORIGIN      
        1 caacacaatt tatagaatcg atttctataa aagaaaaaca agttatatat gttgcaggat
       61 ttccagaagg cagtcaggta gatattcttg ccgttgataa tcaatcggat attgcattaa
      121 taggcagaaa atatggatct caaaaaggaa ttttcttccc tacgtttaac tatccattag
      181 gtaaagccaa agaagttgaa tggggtacat tcgtttatct tgttggatat ccaataaact
      241 ttaaaattat tacaaaagca atcgtaagca gtccacgcag ggatgatgcg ggctcttttc
      301 ttgttgatgc tgtagttaat cccggattta gtggcggact tgtactagcg attcgtgatg
      361 gcgtgcctaa ttttgaatta attggtatgg tacagtgggt gcctgaagaa gaagaaagtt
      421 tgatttatcc tgaaaatcct aaaacaaaat caaagtataa tcccgtagtg ccatacgaag
      481 gtaatttgtt cgtaagaaaa cactcatcaa ttcgttatgg tatcgcaaaa attattcctg
      541 ttgaaaatat tatgactttt cttcttgaaa ataaatcata tcttaacaag caaggatttt
      601 acatttttca ggaataattt taaaaattta gtaacttttt tgacattttt agcgtcttat
      661 gtataccgag attcatgatg accaaagaaa ttaaacaaat agaaattaat accacttgcc
      721 caatttgtaa taaaactatt tcagaattat gggttgcaag gttggattct gtgataggta
      781 ttagatatgc ttatatctgt acgaattgta aaaatctgat taaaataaca aaagaaaaat
      841 atttggatac ttcaaatctt catcaaattc ttttttcaca atcgaccact acatgattgt
      901 aaagtgatct tagttcactc tgaaattatt ctaaaataat gcttccgcca agacaaacat
      961 catccttata aaaaactgca aattgtcccg gtgctattcc tctgtcttct ttttctgtta
     1021 caacttttgc tgtatcacca tcaaaattta aattgcaatt atagaaatgc tcaccatgac
     1081 gaatctttac aagataattt ccatcatctg gtctatctcc ggttatccaa ttatacttgc
     1141 cgactttaaa ttcttttcta gcgcgattag taatattttc ttttgagata taaacaatat
     1201 ttttttcaat atctttactg ataacatacc acggaccacc gcccaatctt aaacctgaac
     1261 gctgaccaat tgtatagaaa taaaatcctt tatgtttacc cattaccttg tttgtgtcga
     1321 tatcaattat ttcgccttct aattcgccaa gatgatgctt aataaaatca gaaaatttaa
     1381 tcttaccaag aaagcaaatt ccctgactgt cttttctggt catatttggt aaatcataat
     1441 tatgagcaag ttctctaatt tgctttttct tataagatcc gattgggaat aaggcacggc
     1501 ttaattgctg ctgcgtgaga tatgccagga aatatgtttg atcttttatc ggatctggag
     1561 aagtttttaa aagaaattta tcatttactt tttcaatttt agcgtaatgc cccgatgcaa
     1621 ctttttcaaa agagttgtcg atcttattta taaactctcc aaactttatt aaacggttgc
     1681 aaaacatatc aggatttggt gttctgcctt cttttatttc agaaattgta taagacacaa
     1741 cagaatccca gtactcagtt tgcatattaa tagtttctaa tggaacatca gcttgtttgc
     1801 aaacagcgcg tgcaaattca agatcttctt cccacgggca gtcaccaaga aatgaaaatt
     1861 catcctgaag ccaaatcttc aaatagaaag cagtaacatc atgtccctca tctttgagca
     1921 aacgaagtgc aaccgaacta tcaacaccac cggaaagtaa tactgcaact ttcatttgct
     1981 ttttatatcc ggtttaattt ttattgattt tatttgctcg attaccttat cataaatttt
     2041 cttatctact ttgcttagaa taagcgtaac aagtttactt gaattattaa tcctgtcaaa
     2101 aaatcccaca cataacttat tttttccatc acaaactttc atcaatccac gatacctgcc
     2161 atcaaatatt ccccctgaaa ttgattcaat atcttttata aatattgtaa tggatttatc
     2221 cctaaaaaga aaatctgtac aaataatttt ttcatcatca atttcaattt tgtatggaac
     2281 aagcttataa aaattgatgt atgatttatt taaaaagtaa agtataaaaa gtgctaacaa
     2341 aaatggtatc aaaataataa gatttttatc tataagagct actaacgata acgtataaag
     2401 aagtaacaat ggactaataa ccaggttacc gtaacgataa ataaatttcc agaataacgg
     2461 gtatgtaaaa gtttgcataa atctttatta atttcttttt ataaatttac tcaagtatag
     2521 ttaaacggta aaatcaatta ttaatgcaca atcaataaaa aagggctcag aagagccctt
     2581 aaataatact ttaaattata ttactattta atttctgcac caataccttt tatctgataa
     2641 atagtaaccg gaccagtaac tgttgctcca tcaaactcgg ttccctgctc ttcagcgacg
     2701 tgtttataat aatcaactgc ttcttcttta gttaattcaa ctttataaac tgttccttca
     2761 acaagtgcat ctttgccttt agcttccacc ggaaaaacaa tatccccatc tttaacttta
     2821 atttttattt tttgattttc agcatcactt ttcaaatcca tccagcagcc tgccattgca
     2881 caaacttcaa caatcttacc ttctactaat actgttttac caacatattc ttcgggctgg
     2941 gcaagaattt ttgaaatact ggttttctct tttaatgaaa tttctttacc atatttatca
     3001 gtttgtgaat atgccgcaac tgacaataac agaaaaaaaa gaattactat tttattcatt
     3061 atatctccta tttagttttt tgttatgaaa tttattaaaa ctttatcgtt acctgatcct
     3121 taaattcaat tttgattccc ttcatatccc cgactacttc tttaaattgt tctaaggttt
     3181 ccggtttact gctctgaaac tttttaaaga catctttgtt cttaataatt tctgagaaat
     3241 caatgtcaag aaatgttact tttgatccat caacaaagga cgcatctgtt tcaacaattt
     3301 tatctttaaa gttaaagtat agtgccattt tcattccatc gaacatttct atcattttct
     3361 gttcggcttc ctcatttaaa gttgtatctt gaaattcagt agtatcttca gattctattt
     3421 tgtcttcagg ctgcggtttg gggaaattaa taaccagggt tgaaggattt ccttttttaa
     3481 agttaaactt caacaaatca ccaggtgatt caacttctgt ttcaccaagc tcagcaccat
     3541 aaggaatttt atcttccggg cttggattaa gttttatttt gttgatatca gaaaaagaat
     3601 acaccgcttt gaaaccttcg taattatctg ttgcaaattt ctcccctgag acatatttaa
     3661 ctccttctcc aaaatttgaa gctttgtctt ttaaatcttt ttcattaaac atattaaact
     3721 cttctgattt tgttgtatca aatgacatag cgaattcttt catcatgtta ataacatcgg
     3781 ttttcatcaa aacagtttct tcaatggttc ctgacccatc tttattaaga tttacagttg
     3841 tatttacctg taaacagcct gtaaaaaaaa tcgagactat gattccgata aataaatagt
     3901 tgatatgctt catttcaatt cctcttggtt ttataataaa ttacttgcaa gctcagccag
     3961 ctccgagcgc tcgccttttt ccagatttat atgtgcgtaa agtttttgtc ctttaaatct
     4021 gtctataaga taagaaagtc cgttcgactg cccgtcaaga taaggatgat caatttgata
     4081 aatatcaccc gtaaacacaa ctttggctcc ttcaccggca cgagttataa tcgttttaat
     4141 ttcatgcggt gtaagattct gcgcttcatc aacaataaaa aatattcttt gtaaacttct
     4201 tccacgtata tatgataaag gttcaacaac taatttctct tctttaatca tattatcaat
     4261 cgcctgatgc tgtttatcat tttcaggaaa ttgatcctgt ataaccttta tgttgtccca
     4321 cagcggctgc atatacggtg caagtttgct ttcaacatca cccggaagat agccaatatc
     4381 tttattgcta agtggaacaa ttggacgcgc aacaaatatt tgcctgtaat ttcgttttac
     4441 atgtaaagca ctcgccaggg caagaagtgt ttttcctgtt cccgctttac cggtcatcga
     4501 tacaagttga atgccgggat tgatcagagc atcaaccgca aatgtttgct ctgcattgcg
     4561 cggagtaatt ccataaatat ttgttttatc aactttaatg ataaattctt tattctgatc
     4621 aagatgtgcg agtacagaac gatttgtatt tcttaatata taatatttgt tcggcagggc
     4681 ttcgccattt aattttttta agaacggctt agcagaaacc tgatacggag gctgaaacag
     4741 tttaactaac aaatcatcat ctgcattttc aatcacccct ttaccgctgt aaagttcttc
     4801 aatactttta accctgtcgg ttgtataatc ttcagcaaga actccgagtg ctttagcctt
     4861 cattctaagg ttaacatctt ttgtaacaag aataacctga tgctttcctt taagcttttc
     4921 atgccattca tacgctacgc ttagaacacg atggtcaggt gtatcatctc taaaagcttc
     4981 acggattgca ggcgccaatc cttttgatat tgcaatgcga agttttcctc ttcccttgcc
     5041 catagatatc ccgccattaa acaatgctga tccggtcatt gaatcaagag tacgtgcaaa
     5101 ctcacgcgca ttaaggttta ttacctgact gcctcgttta aaatgatcaa gctcttcaat
     5161 tactgcaagc gggataataa catcattttc ctgaaacata ttaattgatg ctgcatcgtg
     5221 cagaattacg ttagtatcaa gtacaaatgt ttttggtgtt tttttgtttg atgctgggtt
     5281 ctttttaacg gccatattat taataattag ttattgaatg aatttgcaaa ttacttgcga
     5341 aaagtgattc gggtttttct tcagatagat ttgattcttt ataagtcaaa taagtaattc
     5401 agttctaaat tatgatttat atgcaaaatt cagtaataag attatcaaat tcaaataatt
     5461 attaagataa ttcaatatca gttttaggaa ttgttaaaat gaattcagtg ccagaattta
     5521 ttttgctttt tacctcaata tgccaattaa gtttttcagc taattctttt accaaaacca
     5581 accctaaccc ggttccacct tcacccattg tgcctgttcc tgtagaagct ttgcccagta
     5641 caaaaatatt tcttagctgt gatacctcta ttccaatgcc atgatctttt ataactaatc
     5701 tatacatacc attcgaattc aaaacactta tattaatttc actatgctga tttgaaaatt
     5761 taatagcatt atttatcagg ttaccaatta ttgtttgaat tataaaccta tctgttttta
     5821 ttaatagtgc cactggaaca tcaacattaa tttttatttt tttgttttgt gctgattgaa
     5881 gataaggaaa tatagaagat tcaagaacat cttttataaa gagcctttcg gtataaatat
     5941 caattttatt gtttgataat cttgcccaag tcagcagatt ttctaatagc attatcagat
     6001 tctttgaaga agaattcatc tgacttataa tctctttttt atcattatca ctcattcgat
     6061 catattctga ttcaagaagc tcagaaatat tgacaaaagc cgcgatagga tttctaagat
     6121 cgtgggcaat aatcgaaaag aatttatcct ttgtggcatt caatcctaca agcacttcat
     6181 tatttttact tatctcaatc cggttacttt caatttcttc agttttatcc tgcaaaagat
     6241 tttttgcttt aaggatttta tttctatttc ttaaaagaac ccagataaaa ataatcgagg
     6301 caatagccag caaagcaatt acaagtgcaa ttattctctg ggaaattata attttctttt
     6361 gtaaaacatt ttcattcagt aatagttccg tttcacgttc cttttgtttt agttcatgct
     6421 ctgcttgaat ttctaaaagt ttttcactgt tagaaagtac aaacaggctg tcattagaaa
     6481 ctttatattt ttgatggtaa tcatatgctt ttttataatc ctgtttttta taatagagag
     6541 aagcaagttc accatataca acattttgat cagataatga attcatctct tcactcaatc
     6601 taagtgcttc aattaaatat gattcagctt tattaagact attagtagtg ctgtaaaact
     6661 ttccaagatc agtaagtgaa tgaataatct caagtttaaa atcgctttgc tttgccagag
     6721 ttaaagattc tttaaaataa ttttcggctt tagcaaaatt cttcttatca agctcaatcc
     6781 tgcctaaact tgaatatgcc caaattagac tatttgtatc gtttgaaaat ttagaattct
     6841 ccaatgcttg tttaagatac tcttctgctt tttcattgtt ttttaaagtc aaataaactt
     6901 cagcaatgta actttgaagc aaagctaagg atgacttatc agtatagtca gaatacttta
     6961 gtgcctcatt atagtattga agacatttat cataattgct gttattatca tacataatac
     7021 caatgttaag cagagtatta aaaacagtag cattatctcc catctttttt gcataatcaa
     7081 gcgaacgaag aaaagtctca aatgccagat caacattacc cttcatctgg tagttaatcg
     7141 cctgcgaatt aaatacttta attaaactaa gcgaatcatt aagctcttta tatattttta
     7201 tagcatcatc atagtatttg cctgattcat caatattacc aagatgccat aaagcaacac
     7261 ctttattaaa aacagcatct gctttgctcc agcgattttt tgtatcgtca gcaagtttaa
     7321 tagcctcatc aagatatttt attgcttcat ttggattttc ggaagaaaga aaatatccta
     7381 tttcgaccag caattttatt ttctgttttc cttcagattt gtcaagcagt gcttttaagg
     7441 aatcgacttg agtctgagac tgaacggagt taaaaagata tatgaaacaa aggatgattg
     7501 ggattattct gattttactt ttcattttct attaaaaagt tgttacgatt tttaacacca
     7561 aatttattgg gataaataga attgataaaa ttataaaaag gctatcggat ttataataga
     7621 atatcatata tttgcaaaca aaaataagaa tgccattgct caaaaactat ttaaaaatat
     7681 ttttatgatt ttgaa
//
LOCUS       DASWBY010000177         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_43584, whole genome shotgun sequence.
ACCESSION   DASWBY010000177 DASWBY010000000
VERSION     DASWBY010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43584"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..802
                     /locus_tag="VF870_03220"
     CDS             77..802
                     /locus_tag="VF870_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9251222.1"
                     /translation="MQAMILAAGLGTRLKPITDNLPKALVELNGKTLLEIAIRNLIEN
                     DFRRIIINVHHFADKVKEFLNNNTFAADIFISDESDLLLDTGGGIKHARKFFDDSPVL
                     VHNVDIISNLNLKEFYQYHIIDDALGSLVVSNRESNRYLLFNDDNILCGWQDVKKNEK
                     IIVRDEPSLNQLGFSGIHILNPQLIESFPKEQVFSVIKAYLKIAATEDIHAYVSNDLK
                     WIDVGKVDSLKRAEDLYESISRF"
     gene            856..>1535
                     /locus_tag="VF870_03225"
     CDS             856..>1535
                     /locus_tag="VF870_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251223.1"
                     /translation="MLLIIFSLLLFFASCNTTEPDIKPPAEPDSTTQNFTFETFEFGD
                     GFESNELNDVWIFDENNIWAVGYIRDTTTNNLAVNIIRWNGQKWFGFGNNLTSSGLTG
                     IWAYDTNNVYLASGALRKYENGVIKVIDLTHLTFSTGQAINKLWGSSENNIWGIGPNG
                     TIVHYNGVAWSKIDFDINYRFDAITGSKQTGIAYASATSQEFNTIIVELKDNSATTIY
                     NSANNPNN"
BASE COUNT          547 a          225 c          280 g          483 t
ORIGIN      
        1 acattaaaaa taaatatgat ataaatgttt ttctatcaca cattgaacaa gggattaaag
       61 aagaggttat aagataatgc aggcaatgat tctcgctgca ggattaggaa caagattaaa
      121 gccaataaca gataatctgc caaaagcatt agttgaatta aacgggaaaa ccttgctcga
      181 aattgcaatc agaaatctga ttgaaaatga tttcagaaga attataataa atgttcatca
      241 ctttgcagac aaggtaaagg agtttttaaa caataatacg tttgccgcag atatttttat
      301 ttccgatgaa tctgatttac tgcttgatac aggcggtgga ataaaacacg ctagaaagtt
      361 ttttgatgac tcccctgttc ttgttcacaa tgttgatatc atttcaaatt taaatctaaa
      421 ggaattctat caatatcata ttattgatga tgcacttggc tctttagttg taagtaatcg
      481 cgaaagtaac cgctacctgc tttttaatga tgacaatatt ctctgcggtt ggcaggatgt
      541 aaagaaaaat gaaaagataa ttgttagaga tgaaccgagt ttaaaccaat taggatttag
      601 cgggatacat attcttaatc cgcaattaat tgaatccttt cccaaggaac aggttttctc
      661 tgttattaaa gcatatctta aaattgcagc gacagaagat attcacgctt atgttagtaa
      721 tgatttgaaa tggattgatg ttgggaaagt tgatagttta aaaagagctg aagatcttta
      781 cgaaagtatt tcgagatttt gatattcaaa actgagagtc tttttatgaa ttatcggctg
      841 cttaaatttg aattgatgct gctaataatt ttttctttat tacttttctt tgcctcctgc
      901 aacacaacag aacccgatat aaaaccacca gcagagccag acagcacaac acaaaatttt
      961 acttttgaaa cttttgaatt tggtgatggt tttgaatcta atgagttaaa tgatgtttgg
     1021 atatttgatg aaaataatat ctgggctgtt ggatatataa gagatactac tactaacaat
     1081 ttagctgtta atattattcg ttggaatgga caaaagtggt ttggttttgg taataaccta
     1141 acaagcagtg gattaacagg gatttgggca tatgatacaa acaatgttta cttagcctca
     1201 ggtgctttaa ggaaatacga aaatggtgta atcaaagtaa ttgatttaac tcatttaacc
     1261 tttagcaccg ggcaagccat taataaactc tggggctcaa gtgaaaataa tatatggggt
     1321 atcggaccaa acggtacaat tgttcactat aatggagttg cctggtctaa aatagatttt
     1381 gatataaact acagatttga tgcaattact ggctcaaaac aaacaggcat agcttacgca
     1441 agtgcgacaa gccaagagtt taacacaata atagtagaat taaaagacaa ctctgcaaca
     1501 actatttaca acagcgcaaa taatccaaat aattt
//
LOCUS       DASWBY010000178         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_43616, whole genome shotgun sequence.
ACCESSION   DASWBY010000178 DASWBY010000000
VERSION     DASWBY010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43616"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /gene="truA"
                     /locus_tag="VF870_03230"
     CDS             <1..780
                     /gene="truA"
                     /locus_tag="VF870_03230"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX9251224.1"
                     /translation="NYSRFKLFIEYEGTRYSGWQKQENARTIQGSILKAAQEVFGDAF
                     IDIQGSGRTDSGVHALCQVAHFDVKTVLAPEIIRLKLNDMLPHDITILEVEKTNQNFH
                     ARHDATSRSYIYQISRRRTAFGKNFVWWIKDELDFKKMEQASKLFLGMNNYSSFSDDD
                     PTEKSTKVLIDDIQLKEEGDLILIRIVGSHFIWKMVRRIVGILAEVGRGRKSEKDILF
                     YLNSKTNEPAKFTAPPSGLFLEKVTYTGESIPEELTSLIKV"
     gene            complement(890..>1790)
                     /locus_tag="VF870_03235"
     CDS             complement(890..>1790)
                     /locus_tag="VF870_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX9251225.1"
                     /translation="LDNRPMKYEEFMEMLNQVIFVSATPADYELEMSGGVIVEQIIRP
                     TGLLDPEIEVRPIKGQIDDLIAEIRNKTLLKERTLVTTLTKKMAEDLSDYLDKIGIKV
                     RYIHSDIDSLERVEILRDLRLGEFDVLVGVNLLREGLDLPEVSLVAIIDADKEGFLRS
                     ERSLMQTAGRTARNVNGKVIMYADKITESMRKTIAETNRRRKIQKEYNDKNGIIPATI
                     YKSMEDIMSSTSIADIRKKDDKESYGFSKVAEPILKYMDKEQKEDLVEQLTIEMHNAA
                     KDLEFEKAANLRDEIHKLKKMIG"
BASE COUNT          571 a          331 c          322 g          566 t
ORIGIN      
        1 aattattccc gctttaaact ttttattgaa tatgaaggaa caagatattc gggctggcag
       61 aagcaagaaa atgccaggac aattcagggg tcaattctaa aagcagcaca ggaagttttt
      121 ggtgatgcat tcatagatat acaaggttcc ggcagaacag acagcggggt acacgcactt
      181 tgccaggttg cacattttga tgtgaagaca gttctggcac cggaaattat cagactgaaa
      241 ttaaatgata tgctgccgca cgatattaca attctggaag ttgaaaagac gaatcagaat
      301 tttcatgcaa gacatgatgc cacatcacga agttatattt accagatatc aagaagaaga
      361 acagcttttg gtaaaaactt tgtctggtgg ataaaagatg aacttgattt taagaaaatg
      421 gaacaagcat caaaattatt tttgggaatg aacaattact cttcattttc tgatgatgat
      481 cctacagaaa aatccacaaa ggttctgatt gatgatatac agttaaaaga agaaggcgat
      541 ttaatactta tcagaattgt tggctctcat tttatttgga aaatggtaag aagaattgtt
      601 ggaatattag ctgaagttgg acgtggaaga aaatcggaaa aagatatttt attctactta
      661 aatagcaaaa caaacgagcc ggcaaaattc accgctccgc catcgggatt gtttttggaa
      721 aaagtaactt ataccggcga atcaattcct gaagaactta cgtcattaat taaagtctag
      781 aaaataatgc tgaactaact tcggcatctt tacaaaaatt atgtaggttc tgaaacgagt
      841 tcggggtgac ggataattaa attgccgacc gaggattgaa gaccaaaaac tatccaatca
      901 tctttttaag tttatgaatt tcatcgcgta agttggctgc tttttcaaac tcaagatctt
      961 ttgctgcatt atgcatttct atcgttaact gctcaaccaa atcttctttt tgttctttat
     1021 ccatatactt gagaataggc tcagcaactt ttgaaaaacc gtagctttcc ttatcatctt
     1081 tctttctaat atccgcaatt gaagttgaag acataatgtc ttccatggat ttgtaaatcg
     1141 tagcaggaat aattccgttc ttatcgttat actctttttg aattttacgc cttcggttag
     1201 tttcagcaat agtttttctc atcgattcag tgatcttatc cgcatacata atcactttgc
     1261 cgttaacatt acgggcggtt ctcccggctg tctgcataag tgatcgctca cttcgtaaaa
     1321 atccttcttt atctgcatcg ataattgcaa caagagaaac ctcgggtaaa tctaagcctt
     1381 cgcgtaaaag atttacaccg actaagacat caaattcacc caaacgaaga tcacgaagaa
     1441 tctcaacacg ttctaaagaa tcaatatcgc tgtggatgta tcgtactttt attccaatct
     1501 tatcgagata gtcggaaaga tcttctgcca ttttttttgt taaagttgta acaagagttc
     1561 tttctttaag tagagtttta tttcttatct ccgcaatcag atcatcaatc tgtcctttaa
     1621 tcgggcgtac ttcaatttca ggatcaagta atccggtagg acgaatgatt tgttcaacaa
     1681 ttactccgcc gctcatttcc aattcataat ctgcaggagt agcactgaca aaaattacct
     1741 ggttcagcat ctccataaat tcttcatact tcattggtct gttatcaagt
//
LOCUS       DASWBY010000179         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_194016, whole genome shotgun sequence.
ACCESSION   DASWBY010000179 DASWBY010000000
VERSION     DASWBY010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194016"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VF870_03240"
     CDS             <1..469
                     /locus_tag="VF870_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX9251226.1"
                     /translation="TLLGDGSSSRLFQAVREKLGMTYQINTFLNSYLDVSAFGVYYST
                     NEKQAGKVNEIVHREFTKLRNVEVKAKELKRVKEYIKGNTLMSLESTTNRMIRLANSL
                     LHYGKIVPIEDILNKIDSVTADDIQKLSNEVLNESTLSKIIICSKDNSVQKAA"
     gene            498..2183
                     /locus_tag="VF870_03245"
     CDS             498..2183
                     /locus_tag="VF870_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9251227.1"
                     /translation="MPKFNIDGKEIEFKPGQTIIEAAREYGIEIPHFCWHPKLSVSGN
                     CRMCLVEVEKMPKLVIACSTNAAEGMVVHTESDKSISARNAVMEFFLINHPLDCPICD
                     EAGECKLQDYAYQHSVGESRFVEEKVHKDKRVELGPNVMFDGERCISCSRCIRFCDEI
                     AKDPELTFLKRGDRVTIATFPGEQLDNPYSMNVVDICPVGALTNRDFRFKARVWDMAH
                     TNTICNGCARGCNIEVWTRNNEILRLVPRHNEDVNSYWMCDHGRLNTFKFVNAGDRVD
                     GPHLRVEGNLIKVGWDEAFAKAASSLRTFGKDEIAVLGSAYATVEDNFIAAKFAKSIL
                     GTGHLDFVRHLDPSFGDDILKNEDVTPNSKGAELAGVIPSKNGLDFNGIVKSIREGKI
                     KALYILEDDILNDNPELESVLAKLDLLIINASNFNKTSALADIVFPAAAYAEKNGTFV
                     NFSGRVQRIRPALATLDVDRALEGMSMSRLDKFGTKYDRWAQGKHYDARSSWKILTGL
                     ASAMGQKLKYNMAEEVFYDLSNAVEAFKGLDYDLLGELGVQLNSVNLKSTVKI"
     gene            2205..3260
                     /gene="nuoH"
                     /locus_tag="VF870_03250"
     CDS             2205..3260
                     /gene="nuoH"
                     /locus_tag="VF870_03250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX9251228.1"
                     /translation="MEAIGNILWSIISFYNFLLIRIVIVLVLMLMTAAYLVYFERKIS
                     AWAQNRLGPNRVGWYGLLQSFADVFKLLFKEDIVPEVANKKIHALAPMIALFVAFTTY
                     AVIPIGPGITIAGFDIPMVIADVNIGILYVLALTSIGVYAITFAGWSSGSKYSLLGGI
                     RSSAQMISYEISMGFSIGGVILLSESLRPMAIVESQSGLMWNAFIQPIGFITFLVSAF
                     AETNRLPFDLPEAEPELVGGYHTEYSSMKFAAFFLAEYANMLVASTLIVTLYLGGWQI
                     PYIETLGLSSGLVLALQVAGFFVKVGVLLFFFIWVRWSIPRFRYDQLMNLGWKVMFPL
                     SLFNIIWVAVLIMIFKL"
BASE COUNT         1066 a          483 c          684 g         1085 t
ORIGIN      
        1 tactttactt ggtgatggaa gctcttcaag attgtttcag gcagttagag aaaaacttgg
       61 aatgacttat cagataaata cttttttaaa ttcttattta gatgtttctg cgttcggagt
      121 ttattattca acaaatgaaa aacaggcagg aaaagtaaac gaaatagtgc atcgtgaatt
      181 tactaagttg agaaatgttg aagttaaagc aaaagaatta aagcgcgtta aagagtacat
      241 caaaggcaat acactgatga gccttgaaag tacaactaac agaatgatac gattagcaaa
      301 ttcacttttg cattatggta agatcgttcc gattgaagat atacttaata aaattgattc
      361 agttaccgct gatgatattc aaaagttatc aaacgaagtt ttaaatgaat caacactcag
      421 caaaataatt atttgttcaa aagataattc agtacaaaaa gcagcttaag ttttaagatt
      481 aaaatccaga gtagattatg cccaaattta atattgatgg aaaagaaata gagttcaaac
      541 caggccaaac catcattgaa gccgcacgtg agtatggaat tgaaattcct cacttctgct
      601 ggcatcctaa attatctgta tcgggtaatt gcagaatgtg tttggttgaa gttgaaaaga
      661 tgccaaaact tgttatcgct tgttcaacta atgccgcaga aggtatggtt gttcatacag
      721 aatccgataa atcaatttca gcgcgcaatg ctgtaatgga gtttttcctt atcaatcatc
      781 cgcttgattg ccctatttgc gatgaggcag gcgagtgtaa acttcaggat tatgcatatc
      841 agcacagcgt tggtgaaagc cgatttgttg aggaaaaggt tcataaagat aaacgtgttg
      901 aacttggtcc taacgttatg tttgatggtg agcgttgtat ttcgtgttca agatgtatcc
      961 gcttttgtga tgagatcgca aaggatcctg agttgacttt tcttaaacgc ggtgatagag
     1021 ttactatcgc aacttttcct ggtgagcagc ttgacaatcc ttactcaatg aatgttgttg
     1081 atatctgtcc agttggtgca cttacaaatc gtgattttag atttaaagca cgcgtctggg
     1141 atatggcgca tacaaataca atttgcaacg gctgtgcgcg aggatgtaat atcgaagtat
     1201 ggacaagaaa caatgaaatc cttcgattag tgccaagaca caatgaagat gttaatagtt
     1261 actggatgtg tgatcacgga agattaaata cttttaaatt tgttaacgca ggcgatagag
     1321 ttgatggccc tcatttaaga gttgagggaa atttaattaa agttggttgg gatgaagctt
     1381 ttgcaaaagc agcatcaagt ttaagaacat ttggtaagga tgaaatagct gttcttggtt
     1441 ctgcatatgc aaccgttgaa gataatttta ttgctgcaaa atttgctaaa tcaattttag
     1501 gaacaggaca tttagatttt gttcggcatc ttgatccatc ttttggagat gatattttaa
     1561 aaaatgaaga tgtaacccca aattctaaag gtgctgagtt agcaggtgtc attccatcaa
     1621 aaaacggatt agattttaat ggaattgtaa agtcaatccg tgaaggaaaa ataaaagcac
     1681 tttatatttt agaagatgat attttaaatg ataaccctga attggaaagt gttttagcta
     1741 aacttgattt attaataatc aatgcaagta attttaacaa aacttctgcc ctggctgata
     1801 tagtttttcc ggcagctgct tatgcggaaa aaaatggtac atttgttaat ttttccggaa
     1861 gggttcaaag aattcgtcct gcacttgcaa ctcttgatgt agatcgtgct cttgagggaa
     1921 tgtcaatgag tcgtttagat aaatttggca ctaaatacga tcgctgggca caaggtaaac
     1981 attatgatgc tagatcaagc tggaaaatat taactggatt agcttctgca atgggacaaa
     2041 aattaaaata caatatggct gaagaagtat tttatgattt atcaaatgct gtagaagctt
     2101 tcaaaggatt ggattacgat ctgttgggag aacttggtgt acaattgaat tcagtaaatt
     2161 taaaatctac tgtaaaaatt taggttataa ggaattaaaa attaatggaa gcaatcggaa
     2221 atattttgtg gtcaatcata agcttttata attttttatt aataagaatt gtaatagttc
     2281 ttgtgctgat gttgatgaca gctgcttatt tagtttactt tgaaagaaaa attagtgcat
     2341 gggcgcaaaa tcgactcggg cctaatagag ttgggtggta cggattgttg cagtcttttg
     2401 cagatgtttt taagctttta tttaaagaag atattgttcc ggaagttgcc aacaagaaaa
     2461 ttcatgctct tgcaccaatg attgcactgt ttgtagcgtt cactacttat gctgtgattc
     2521 caatcgggcc tggaattaca attgcaggtt ttgatattcc gatggttatt gctgatgtaa
     2581 atatcggaat tctttatgtt cttgctttaa catcaattgg agtttatgca attacatttg
     2641 ccggatggtc atcaggaagt aaatattctt tgttaggcgg aatcagatcc tctgcacaaa
     2701 tgatttctta tgaaatttcg atgggatttt ctatcggcgg tgtaattctt ttatcagaat
     2761 cattaagacc gatggcaata gtagaatcac aatccggttt gatgtggaac gcatttatac
     2821 agccaatagg atttataaca tttcttgtat cagcatttgc agaaactaac agattaccat
     2881 tcgatcttcc agaagcagaa ccagaacttg taggtggtta tcacactgaa tacagcagta
     2941 tgaaatttgc cgcattcttt ttagcagagt atgcaaatat gttagttgcc agcacactta
     3001 ttgtaaccct gtaccttggc ggatggcaaa tcccttacat agaaactctt ggcttatcat
     3061 caggattagt attagcactt caggttgcag gattctttgt aaaagttgga gtattactat
     3121 tcttctttat ctgggtaaga tggagcatac caagattcag atatgatcag ctaatgaatt
     3181 taggctggaa agtgatgttc ccattatcgt tgtttaacat tatttgggtt gcggttttaa
     3241 tcatgatctt taaattataa tttaatattg ttcagttatg aataagagag aaaatattaa
     3301 caggagtttt gatgtcag
//
LOCUS       DASWBY010000180         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_572117, whole genome shotgun sequence.
ACCESSION   DASWBY010000180 DASWBY010000000
VERSION     DASWBY010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572117"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2136)
                     /locus_tag="VF870_03255"
     CDS             complement(<1..2136)
                     /locus_tag="VF870_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HEX9251229.1"
                     /translation="MAFSQQKDSLSTSKNLIGKTIDSLFVSKDKISSPVDSLFDAKLK
                     GKKYDVDTTVFASAKDSLIFLVKDKKMKIYGDGSINYKTTEIKSSNIFIDFDSNNIDA
                     VGVPSDTLPGKFDGTPVLKEGAEVYAGQTMKYNFKSGQGLISLAKTEQDGSKYTGTKI
                     KKVDQETYFIEGGIFTTCDNEECPHYHFTASKMKVIQKDQLIADWIFINFGGVPLPIP
                     LPFAVFPIESGRRSGIIPPTFGSDPNYGTYFSGFGYFWAINDYTDVNLKGDYYTRGSY
                     KFDTRFRYSKRYDYSGNLEGSYGYFVKGEDTDPDRVENKEWRIAWNHNQAITPTSRFD
                     AKLEFISGINIRRDINDLNEILRNDAVSNATYFKQWEESGNSLSLSYSRNQNFESNDI
                     SEVLPNLTFSLAQSYPFRDNLGKQDWYETFGFNYSGQLQNNRNKIDGNLKTRGGIQHN
                     ITASLSPKIGYFSISPNLRYNESWYNKYVTKYTTTDTSGKYYVQTDDMQGLNAVRTFS
                     MGVAASTKFYGMFNINSLGINAIRHTVTPSISYNYSPDFSTPFWDYYASYSDSTGQFV
                     EYNKFEREIFVKPSNQEQQSINFSLGNVFDIKTNPDLTDTTSKENKFQLLNLTASMGY
                     NFAAKTFKFSDLNLSYRTQIGSLLSFSGSSTFSPYDYDEKGRIDRYLISNGGGLLRLT
                     NTNFSVSLSLAGDKLTSSSKKTNIQQDEYL"
     gene            complement(2472..>2672)
                     /locus_tag="VF870_03260"
     CDS             complement(2472..>2672)
                     /locus_tag="VF870_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9251230.1"
                     /translation="SNLLDISTFIIGGGVAGFGKPLFDSTRVTISERVLLPLRSRVLV
                     LPAKLQNEAGIKGASSLVFYNN"
BASE COUNT          857 a          496 c          413 g          906 t
ORIGIN      
        1 taaatattca tcctgttgta tgtttgtttt cttgcttgat gaagtcaatt tatctccagc
       61 aagcgataag gaaacagaaa aattagtatt tgtcaatctt aataatcccc cgccattgct
      121 tattaaatat ctatcgatcc tgcccttttc atcataatca tacggggaaa aagtagaact
      181 acctgaaaaa cttaataagc tgcctatttg agttctataa ctcagattca agtccgaaaa
      241 tttgaaagtc tttgctgcaa agttatatcc cattgatgcg gttagattta atagttgaaa
      301 tttattttct ttggatgttg tatccgtaag atcaggattt gttttaatat caaaaacatt
      361 acctaaagaa aagttaatac tttgctgctc ctgatttgat ggttttacaa atatttctct
      421 ttcgaattta ttatactcta caaattgacc tgtactatcc gaatacgaag cataataatc
      481 ccagaatgga gttgaaaaat caggagaata attataactg atagatggtg taactgtatg
      541 tcttattgca tttataccaa gcgaattaat attaaacatt ccataaaatt tagtagaagc
      601 ggccacaccc atcgaaaatg ttcgtacagc atttaatccc tgcatatcat ccgtttgaac
      661 atagtatttt ccagatgtat cagtcgttgt gtactttgta acatatttgt tgtaccagct
      721 ttcattgtaa cggagatttg gagaaatact aaaatacccg atctttggag ataacgaagc
      781 agtgatgtta tgttgaattc cacctctggt ttttaaatta ccatcaattt tgtttctgtt
      841 attctgcagc tgtcctgaat agttaaaacc aaaagtttca taccaatctt gtttacctag
      901 gttatctcta aaaggataac tctgagcaag tgaaaaagtt aaatttggta atacttcaga
      961 aatatcattt gattcaaaat tttgattgcg tgaatagctt aatgataaac tgtttcccga
     1021 ttcttcccac tgcttaaaat atgttgcatt agatacagca tcattcctta aaatttcatt
     1081 aagatcattt atgtcacgtc ttatatttat gcctgagata aattctaatt ttgcatcaaa
     1141 ccttgatgtt ggtgtgatag cttgattatg attccacgca atacgccact ctttgttctc
     1201 cactctatcc gggtcagtgt cttcaccttt aacaaaataa ccatagcttc cttcaaggtt
     1261 accggaataa tcatatcgtt ttgaatatct gaatcttgtg tcaaacttat agcttccccg
     1321 tgtataataa tcacccttta aatttacgtc tgtgtaatca ttaatcgccc aaaaatatcc
     1381 aaaccctgaa aagtacgttc cataatttgg atcgctgcca aatgttggtg gaatgattcc
     1441 tgaacgtctt cccgattcaa tcgggaaaac tgcaaatggc aaaggtattg gcaaaggtac
     1501 accgccaaaa tttataaata tccaatcagc tataagctga tctttttgaa ttaccttcat
     1561 cttacttgca gtaaaatgat agtggggaca ttcttcatta tcacaggttg taaaaatacc
     1621 accttcaata aaataagttt cctggtccac ttttttaatt ttcgttccag tgtattttga
     1681 tccatcctgc tcagtttttg caagtgaaat caatccctgt ccggatttaa aattatattt
     1741 cattgtttgg cctgcatata cttccgcacc ttcttttaaa acaggtgtac catcaaactt
     1801 accaggaagt gtatcagaag gaacgccgac tgcatcgata ttattggaat caaagtcaat
     1861 aaaaatgttg gagcttttaa tttctgttgt tttataattg atgcttccat cgccgtaaat
     1921 cttcatcttt ttatctttta caagaaagat taaagaatct ttagcagatg caaaaactgt
     1981 tgtatcaaca tcatattttt tgcctttaag ttttgcgtca aaaagtgaat caacaggact
     2041 tgaaatttta tcctttgaaa cgaaaagtga atcgattgtt ttaccgatta gattttttga
     2101 ggtagaaagt gaatcttttt gctgcgagaa agccacattg gagttaatca ataggattaa
     2161 tattaagatg caataactca cctttaatcc ctctcttaaa aaaagtagga tttctgagat
     2221 ataaattcta ttccccttct ctttgcaaga aaaggagtcg tgggatgagt tcaaagcctt
     2281 atacaatatt tatgattaat tataattaaa actaaatatc atccggagcc ctgataaatt
     2341 gggataaact ccgaagaagg atgacacatt ttcataacac aattaccact cttcatctct
     2401 cctctcggct cgaaatacca agcactcttc atcaattaat ttaaatacta caacaagtat
     2461 tatagccaag attaattatt ataaaacacc aatgatgatg cgccttttat tccggcctca
     2521 ttctgtaatt tagcgggaag aacaagcact cttgagcgca aaggcaaaag cactctttca
     2581 gaaatcgtta cgcgtgttga atcaaacaaa ggcttgccaa atcccgcaac accaccgccg
     2641 ataataaatg tgcttatgtc cagtaaattg ct
//
LOCUS       DASWBY010000181         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_232401, whole genome shotgun sequence.
ACCESSION   DASWBY010000181 DASWBY010000000
VERSION     DASWBY010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232401"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VF870_03265"
     CDS             complement(<1..565)
                     /locus_tag="VF870_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX9251231.1"
                     /translation="MALKKKKSVKEKNGKNSSNKNYFILTSENKKQILGIFLLITSVI
                     LFLSVITFDRRDEANLSNSYISDFFKSFDTKIDIQNWLGVTGAHFSLFLVKATFGYFS
                     ISIPLVLFLWGISFFKKIDFKFRLHVSNFLLLSSIILASFFGVLQVNYGLFSSTYELS
                     GFIGNHLGNFLSGLVGGIGSILILTGSF"
     gene            complement(584..1312)
                     /locus_tag="VF870_03270"
     CDS             complement(584..1312)
                     /locus_tag="VF870_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX9251232.1"
                     /translation="MKLNVFLSPVIVDELYFTGKTTVVIDVLRATSTIVTAISNGAKE
                     VVPVASVEFAVKVSGGMFGGQILLGGERNTKKIEGFALGNSPFEYSKEVVDGKTIVFF
                     TTNGTKSIAKAKYSENLYTCSFLNISAVAKHLITLNTDVEIVCAGRNNYFSIEDSVCA
                     GMLISKISKVKADSFLNDSGAAVLTLYEKYGANLIKMMRESDHGKILLENDFEADLEY
                     CSSIDILDVIPSYANGVLKKLKSN"
     gene            complement(1320..>1935)
                     /locus_tag="VF870_03275"
     CDS             complement(1320..>1935)
                     /locus_tag="VF870_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX9251233.1"
                     /translation="LTDTQINLEYYHFTKINIAGIDMIFSRTGYTGELGYELYFTGDE
                     KVAEELWNKIFEAGKEFDIQPVGLAARDSLRLEMGFCLYGNDIDQTTNPLEAGLGWIT
                     KLSKSEFIAKDILVKAKADGLNRKLVALTSEAKIFPRHGYDITFNNNKIGVITSGTVS
                     PMLDQAIAMGYVETKYSEIGSEVNLMIRGKEVPAQVVKLPFVKR"
BASE COUNT          647 a          346 c          283 g          659 t
ORIGIN      
        1 taaaagatcc ggtaagaatt agtatacttc caattccacc gactaatccg cttaaaaaat
       61 tacccaaatg atttcctata aatccagata attcataagt agaagagaat aaaccataat
      121 ttacctgaag cacaccaaaa aatgatgcca atatgattga gcttaatagc agaaagtttg
      181 aaacgtgaag tctgaatttg aaatcaattt ttttaaagaa tgaaattccc cataagaaga
      241 gtacaagtgg aattgagatt gagaaatatc caaaggttgc tttaacaaga aatagagaga
      301 aatgcgcacc tgttacaccc agccagtttt gtatatcaat tttagtatcg aatgatttga
      361 aaaaatctga aatgtaagag tttgaaaggt tggcttcatc ccttctatca aaagtaatta
      421 cgcttaaaaa aaggataaca gaagttatca ggaggaatat tccaagtatc tgttttttat
      481 tctcagacgt taaaataaaa tagtttttat tggaactatt ttttccgttt ttttctttaa
      541 cgcttttttt ctttttaagt gccattaatt tttatttaca gaattaattg ctttttaatt
      601 tttttaaaac accgttcgca taagaaggaa taacatcaag aatatctatc gaactgcaat
      661 actctagatc tgcttcaaag tcattttcta aaagaatttt tccatgatca gattcgcgca
      721 tcattttaat taaatttgca ccatactttt catataaagt taaaacggcg gctccggaat
      781 cattcagaaa agaatctgct tttaccttac taatttttga aataagcatt ccggcacaaa
      841 ctgaatcctc aatagagaaa taattatttc tgcctgcaca gacaatttcg acatcagtat
      901 ttaatgttat aagatgttta gctactgcgc tgatatttaa aaaagaacac gtgtataaat
      961 tttctgaata ctttgcttta gcgattgatt ttgttccatt agttgtaaag aacacaatag
     1021 ttttaccatc aacaacttct ttagaatatt caaatggtga attaccgagt gcaaaacctt
     1081 caattttttt tgtgtttctt tcaccgccta ataatatttg gccgccaaac attccgcctg
     1141 aaactttaac agcaaactca acacttgcaa ctggtacaac ttcttttgcg ccattactaa
     1201 tagcagttac aattgttgat gttgcacgaa gcacatcaat tacaactgta gttttgccgg
     1261 taaagtagag ttcatcaaca attactggtg aaagaaatac gtttaacttc atgttatttt
     1321 tatcttttta caaacggaag ttttaccacc tgtgccggaa cttcttttcc tcttatcata
     1381 agattaactt cagaaccaat ctcagagtat tttgtttcta catatcccat tgcaatagcc
     1441 tgatcaagca tgggacttac tgtaccgctt gtgataacac cgattttatt attgttaaag
     1501 gtaatatcat aaccatgtct tggaaatatt tttgcttcag aggttaaggc taccaatttt
     1561 ctgttaagtc catcagcttt agcttttacc aaaatatcct tagcaataaa ttcagactta
     1621 gaaagttttg ttatccatcc caggcccgct tctaatgggt ttgtggtctg atcaatatca
     1681 ttaccataaa ggcaaaaacc catttccaat cttaaagaat cacgtgcagc taatccaacc
     1741 ggttgaatat caaattcttt tccggcttca aatattttat tccataattc ttctgcaact
     1801 ttttcatcgc cggtaaaata gagttcataa ccaagttcac cggtataacc ggttcttgaa
     1861 aaaatcatat caataccggc aatattaatt ttggtaaaat ggtaatactc gagattgatt
     1921 tgtgtatctg taagt
//
LOCUS       DASWBY010000182         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_232535, whole genome shotgun sequence.
ACCESSION   DASWBY010000182 DASWBY010000000
VERSION     DASWBY010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232535"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VF870_03280"
     CDS             complement(<1..1058)
                     /locus_tag="VF870_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX9251234.1"
                     /translation="MKKIFLLFIILTSTNLFSQNWLKVDSIFAVSGVTVQSFSAPEFA
                     DLNNDGNPDLILGNIGDEADFFWNNSHSFPTTFSKDTSLFYNIYHSGQTNTNSDYPTL
                     IDLDGDGDLDLVIGGFNGLRYYKNDGTVISPDFVPADGIFDAVNSMVGTDAQPAFVDI
                     DDDGDLDLFMGIGESFYGGVTAGITMGFRNTGNSRDPIFTLDNALVTGIPDIGLNSYP
                     TFADLDNDGDYDLLLGRDLQTFVYFRNTGTAQNPVWTQNSTTFSGVETTTYWKNPTFA
                     DLDYDGDFDLVYGTDGGMLYFYRNIGTVTIPSFQYYPDYFKIIKLSGGSATVSLKDYD
                     GDGDNDLLSGDWLGKFNY"
     gene            complement(1182..1307)
                     /locus_tag="VF870_03285"
     CDS             complement(1182..1307)
                     /locus_tag="VF870_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251235.1"
                     /translation="MSKGQDSKKSTKKEPAKTMKEKKAAKRDKKNEKGGYSILNK"
     gene            complement(1617..2864)
                     /locus_tag="VF870_03290"
     CDS             complement(1617..2864)
                     /locus_tag="VF870_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9251236.1"
                     /translation="MNIKTIFNSSSSQKHPHPSVFMFLFLPFGVMGGYVSVTIGFLLT
                     KAGVPLVQVAPIIAVTLLPNIFKFVWAPLVDTTLSVKKWYLISNVITAIGILATGIIP
                     LKVEYLTLMTIVVFITSLVNTFVAMATESLLAHDTPDNLKGRAGGWLQAGNLGGLGLG
                     GGAGLWLAERLPEPWMAGAVIAFACLFCSLGLPFLSEPTSFIREKNYLKTIGNLNKDI
                     WTLIKSRMGFLALFLCFLPIGSGAASNLWSSISNDWGASADTVALLVGVVGGVLSAIG
                     CLAGGWICDLMDRKKAYILFGMIQALCAVGMAFSPATEQMFIVWTSLYALSTGLTYAG
                     FSAFVLEAIGKGAAATKYNVFASLSNAPIYYMIYMDEWSHGKWGAFGMLSTEAIMALF
                     GMILFITIFVTVNKMRPAEEIAI"
BASE COUNT         1036 a          595 c          500 g          982 t
ORIGIN      
        1 aagtaattaa attttccaag ccaatctccg cttaataaat cattatcacc atctccatca
       61 taatctttaa gagaaacggt tgcacttcct ccgcttaatt ttatgatttt aaagtaatca
      121 ggatagtatt ggaaagatgg tattgttact gttccgatgt ttctgtaaaa atatagcata
      181 ccgccgtctg taccgtaaac aagatcaaaa tctccgtcgt aatctaaatc tgcaaatgtt
      241 ggattcttcc aataagtagt tgtttctact ccggaaaaag tagttgaatt ttgtgtccac
      301 acagggtttt gcgctgttcc tgtatttcta aaataaacga atgtttgtaa atcccttcca
      361 aggagcaaat cataatctcc atcgttatct aaatccgcaa atgttggata agaatttaga
      421 cctatatcag ggatgccggt taccaaagca ttgtccagag taaaaattgg atctcttgaa
      481 tttccggtat ttctaaatcc catagtaatt ccggctgtaa ctcctccata aaatgattca
      541 ccaatcccca taaataaatc aagatcaccg tcatcatcga tatcaacaaa agctggctga
      601 gcatcggttc caaccataga atttactgca tcaaatattc catcggcagg aacaaaatca
      661 ggcgaaataa ctgttccatc atttttgtaa taacgcaaac cattaaagcc gccaattact
      721 aaatcaagat caccatcacc atctaaatca ataagagtcg gataatcaga attagtattt
      781 gtttgcccgc tgtgataaat attataaaac agggatgtgt ctttgctgaa tgtcgttggg
      841 aaggaatggg aattattcca gaaaaagtct gcttcatcac caatattgcc aagtatcaga
      901 tccggattgc catcattatt taaatcagca aattccgggg ctgaaaagct ctgaacagtt
      961 acgccagaaa cggcaaatat cgaatcaact tttaaccagt tttgtgaaaa aagatttgtt
     1021 gaagttaaga tgataaaaag taaaaatatt tttttcataa cacttcccgg ataattttta
     1081 ataacgcaaa caaaaataga tattttatta tgatgtagaa attctcagtt gaattaaatc
     1141 gaggcgatat ttgaaattat ttaatacgct gcaatactta tttatttgtt aagaatacta
     1201 taaccgccct tttcattttt cttatcacgt ttagccgctt ttttttcttt catggttttt
     1261 gccggctctt tctttgtgct cttcttacta tcctgtcctt tgctcataac attttccttt
     1321 tggtttagat attaatttct actgctctac aataatgata attaaactta atagataggc
     1381 tcaataagat atatttcgaa ataaatttcc ggattgaaat tgcatttagg tatgatggat
     1441 gaaaatcact tcatcattat ttatatgcag gattaaacaa aaccaatatt gtaactagtc
     1501 gtttgtttag tggcaggttc actatctcaa ttttcattag gtctcgtcgt tccacttgaa
     1561 acaactattg tcaagctgat attttagaaa tagtctggaa ataatggatt gcatttttaa
     1621 atggctattt cttctgcagg tctcattttg tttaccgtta caaaaattgt tatgaataag
     1681 atcatcccga aaagtgccat tattgcttct gtagaaagca taccgaatgc accccatttt
     1741 ccgtgtgacc attcatccat ataaatcata taataaattg gtgcattgga taaagaagca
     1801 aatacattgt acttagttgc tgcggcacct ttacctatgg cttcaagaac aaaagcacta
     1861 aatccggcat aggttaaacc agtgcttaat gcgtaaagag aagtccatac aataaacatt
     1921 tgttctgtgg cgggactaaa tgccatcccc accgcacaaa gtgcttggat cattccaaat
     1981 aatatatacg cttttttgcg atccataaga tcgcatatcc aaccacctgc taaacatcca
     2041 attgcagaaa gtaccccgcc aactactccg actaaaagtg caactgtatc ggcagaggca
     2101 ccccaatcat ttgaaattga agaccataaa tttgaagcag caccagatcc gattggaagg
     2161 aaacataaaa acagtgctag aaaacccata cgagatttta taagggtcca gatatcttta
     2221 tttaagtttc caatagtttt taagtaattt ttttctctaa tgaacgatgt aggttctgaa
     2281 agaaaaggta aaccaaggct gcaaaataga caagcaaatg ctataaccgc acccgccatc
     2341 catggctctg gtagtctttc agccaaccat aaaccagcac ccccgcccaa tcccagtccg
     2401 ccaagattac ctgcttgaag ccaccctcct gctctacctt ttaaattatc cggtgtatca
     2461 tgagccaata agctttccgt agccatagca acaaaagtat ttactaagct ggtaataaat
     2521 acaacaatag tcatcaatgt cagatattca acttttaatg gaataattcc cgtggccaag
     2581 atgccaattg ctgttattac attagaaatt aggtaccact ttttaacaga tagagttgta
     2641 tcaactaggg gtgcccaaac gaacttaaat atatttggca acaatgttac tgcaataata
     2701 ggagcaacct gtactaatgg gactcctgct tttgtcaaca ggaatccaat ggtaacactt
     2761 acatatccac ccattacccc aaatggaaga aataaaaaca taaaaacact tgggtgtgga
     2821 tgcttttgcg atgagcttga gttgaatata gtttttatgt tcatttaata aattcctttg
     2881 atcaattgct tttttgctta atctatttat aagaaaaacc ataggattaa ttactgttga
     2941 agataatcaa aatactttgc attacaagaa gcaaatattt acgccacgca aatgatgtga
     3001 gtcatatcgg gttggaagaa ttctattttc atttgttgtt tcttaatggt ataagaagtt
     3061 aaatgtatta agtaaaaaaa aatttattca gaactttagt cagaagtgta agt
//
LOCUS       DASWBY010000183         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_158345, whole genome shotgun sequence.
ACCESSION   DASWBY010000183 DASWBY010000000
VERSION     DASWBY010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158345"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VF870_03295"
     CDS             complement(<1..477)
                     /locus_tag="VF870_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX9251237.1"
                     /translation="MTETNVPLIIAHRGESVEAPENTLSAINLAWERGADGVEIDVHL
                     TRDNQIVVIHNSKTRIISGKSLSIKSQTLGKLKSFDVGRKIGDKWYAERIPTLDEVLS
                     TVPPDKYLFIEIKCGLEIVPQLKQVFKQTKLDTNQIKIIGFGLKKMTVIKKNFSEYE"
     gene            complement(508..1626)
                     /gene="cfa"
                     /locus_tag="VF870_03300"
     CDS             complement(508..1626)
                     /gene="cfa"
                     /locus_tag="VF870_03300"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEX9251238.1"
                     /translation="MNKDEVFVKDILSLADVKINGINPWDIQLKNNNFYKRVLSDGSL
                     GLGESYMDGWWECEDLPELFFKILTADLEHKVVPLKLLFMVLKSKIFNLQNQGRAARD
                     VGYHYNLGNVLFQNMLDKYMAYSCAYWKDADNLEAAQVNKLKLICDKIYLKPGMNLLD
                     IGCGWGSLMKYAAENFGTKSLGITLSQDQLELGSKLCKDLPIEIQIKDYRDIDGKYDR
                     IVSVGMIEHVGSKNYRTFFEIVNKCLKDDGLFLLHTIGTLNPKTANADPWTNKYIFPN
                     GELPTVQRIAKAAEGLFVMEDWHNFGVDYAKTTLAWYENFNRNWDKIKSKQYDDRFYR
                     MWKYFLLTQSGAFRSRRNNLWQIVFSKRGVPGGYTSIR"
     gene            complement(1963..2526)
                     /locus_tag="VF870_03305"
     CDS             complement(1963..2526)
                     /locus_tag="VF870_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251239.1"
                     /translation="MYTHKNIIHSSLLILFLLIAGCATSYAPDNWLPETDKVPQNNYG
                     GWITVIVNESKDETEDIVLQYGGEFIAEDSLNVYLLYDSLYTISKDKIENSIIELDQK
                     NTGSYAAWVAGGSLLTLSNGYYAAITFPLWMVAGIPATSGESFRDRYEADYPDEFYWD
                     NVKIFSRFPQGIDGINLSELRPLIYNK"
     gene            complement(2513..>2992)
                     /locus_tag="VF870_03310"
     CDS             complement(2513..>2992)
                     /locus_tag="VF870_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251240.1"
                     /translation="PLKNLIKERCNVSDESQINIRLRMAKGLWRIDYAALAELGDKVD
                     PIIIKPSSSFPQKLNSSDVVELLNNQDSVLITFPGDEYFLNYKLPPDFTEYELFMESQ
                     GYYLEWMRNEWLGEENSSKVYQMLFNPVQFYKDLAPQFKKVETDMEETFWSSKYVYP"
BASE COUNT          946 a          571 c          447 g         1028 t
ORIGIN      
        1 ttcatactcg gaaaaattct tttttattac tgtcattttc ttcaagccaa atccaattat
       61 tttaatttga ttggtatcca gcttagtttg cttgaacact tgtttaagtt gtggaacaat
      121 ctctaatcca cacttaatct cgataaaaag atatttatca ggcggtactg tagataatac
      181 ttcgtccaaa gtcggaattc tctcggcata ccatttatct ccaatctttc tgccaacatc
      241 aaagctttta agttttccta aggtttggga ttttatactg aggcttttac cggaaattat
      301 tctagtcttg ctattatgga taacaacgat ttgattatcc ctggtaagat ggacatcaat
      361 ttctacacca tctgcgcctc tttcccacgc aagattgatt gcagacaaag tattctctgg
      421 tgcttcaaca gactcacccc gatgtgctat tattaaaggt acatttgttt cggtcataag
      481 aacattgcaa ttcatgtgaa aataatatta acgtattgaa gtatatccgc caggcactcc
      541 acgttttgaa aaaactatct gccataaatt atttctgcgt gatctaaatg cacctgattg
      601 agtaagcaga aaatacttcc acattcggta aaatctgtca tcatattgtt tggatttaat
      661 tttatcccaa ttgcggttaa aattttcgta ccaggctagt gttgttttag cataatcaac
      721 tccgaaatta tgccaatcct ccatcacgaa aagtccttca gctgctttag caattctttg
      781 aaccgtgggt aattcaccat ttggaaaaat atatttattc gtccatggat cagcatttgc
      841 ggttttagga tttaatgttc ctattgtatg aagtaaaaaa agtccgtcgt cttttaaaca
      901 cttattcacg atttcaaaaa aagttctata attttttgaa ccaacatgtt ctatcattcc
      961 aactgataca atgcgatcat acttgccatc aatatcacga taatctttaa tctgaatttc
     1021 tatcggaaga tctttgcaga gtttacttcc aagttccaac tgatcctgcg atagtgtaat
     1081 accaaggctc tttgttccaa aattttctgc agcgtatttc attaaacttc cccagccgca
     1141 tccaatgtca agtaaattca ttcccggctt taaataaatt ttatcacata taagttttaa
     1201 tttatttacc tgtgcagcct cgagattgtc agcatctttc cagtacgcac aggaatatgc
     1261 catgtattta tccagcatat tctgaaaaag tacattgcca agattataat gataaccaac
     1321 atcccgtgct gctcttcctt gattttgaag gttaaagatt tttgatttga gtaccatgaa
     1381 taaaagtttt aatggaacaa ctttatgttc aagatctgct gttagtattt taaagaaaag
     1441 ttcaggaagg tcttcgcatt cccaccagcc gtccatgtaa gattcaccca gcccaagtga
     1501 tccgtcactt agtacacgct tataaaaatt attattctta agctgaatat cccacggatt
     1561 gatgccatta attttaacat ccgccaaact aagaatatct tttacgaaaa cttcatcttt
     1621 attcatgaca ttgctccttc attaaattta aatgaattag aatcataaat tgtattaact
     1681 attaagtatt aatagcttaa ctcagcttcg gtagagtatt ctggtgtatt atttctgcaa
     1741 gttatttagt ctccaaactc taaacgcttg ctctatcgga gtagtattat tttaacacct
     1801 ctaattagta attaatttta attataagca aataaattaa caatgataat tgccaagtgt
     1861 aaacaatcag caacgatctc tgcaaaacaa actgatcatg aaggaaataa ttgataatta
     1921 atttattccc aatttagaaa aatgaaaaga ttacaaatat tattatttat tataaattaa
     1981 tggcctaagc tctgataaat ttattccatc tataccctga ggaaagcgcg aaaatatttt
     2041 aacattatcc caataaaatt catcgggata atctgcttca tatctgtctc taaaagattc
     2101 accagatgtt gcggggatgc ctgcaaccat ccataatgga aaagtaatag ctgcatagta
     2161 cccatttgat aatgttaaaa gtgaaccgcc agcgacccag gcagcataag accccgtgtt
     2221 tttctggtca agttctataa tgctgttttc tattttatct tttgagattg tatataatga
     2281 atcatataac agatagacgt ttaaggaatc ttccgctata aattctccgc catattgcaa
     2341 cacaatgtcc tcagtttcat ctttagattc attaactata acggttatcc aaccgccata
     2401 gttattctga ggtactttgt ctgtttcagg cagccaatta tctggggcat atgaggttgc
     2461 acaccctgct attaatagaa ataaaatcag caacgaagaa tgaataatat ttttatgggt
     2521 atacatattt actactccaa aaagtttctt ccatatcggt ttcaactttt ttaaattgtg
     2581 gtgcaaggtc tttgtaaaat tgtactggat taaaaagcat ttggtagact ttggaagagt
     2641 tctcctcgcc aagccattca tttctcatcc attcaagata atatccctga ctttccataa
     2701 ataattcata ttcagtaaaa tcaggtggaa gtttataatt aaggaaatat tcatcaccgg
     2761 ggaaagtaat caatacagag tcctggttat ttaatagttc tacgacatcg cttgaattta
     2821 atttttgcgg aaaagaactt gaaggtttta taataatcgg atcaacttta tcgcccaact
     2881 ccgcaagggc agcataatca atcctccata atcctttagc cattcttaat cttatattaa
     2941 tttgagattc atccgaaaca ttgcacctct ctttgattaa atttttgagc gg
//
LOCUS       DASWBY010000184         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_308211, whole genome shotgun sequence.
ACCESSION   DASWBY010000184 DASWBY010000000
VERSION     DASWBY010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308211"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VF870_03315"
     CDS             complement(<1..1361)
                     /locus_tag="VF870_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX9251241.1"
                     /translation="MLQKYFPFLLFVLILFNEQNSAQIKTLDDFENSNGWSEIKADGV
                     TLNIATDAGINGNAIRFDYNFTKGTGYGGIQKFFPIDVPENYEFTFYLKAESPANNFE
                     IKFIDSTGNNVWWVNNRNYDFPKEWRKVRVKKRHINFAWGPTNDQALKRIDRIEFTIA
                     SFVGGKGTIWIDDLKFEPLPPETYDYPLPLVTAKSQPFNLEPKNVVDGSLETYWKSVG
                     VKNQSITIDFKTRREFGGLKIDWLKGYEAASFEVLLSDDGKRWEKVYSVSSNRSNVSF
                     IKLTEAESRFLRINLLKSNSEENFGISEIKVLDIKNSLTPNDFLIYTAKNSSKGDYPR
                     YFLDEASFWTVIGVNNDVKEALINEDGIVEVDKSAFSIEPMLQIGNDYFNWANVSAAQ
                     SLENGYLPIPKVTWNCSDVNLEVEAFANGEANINSTLYLKYRITNISKEQKEGKLFLL
                     VRP"
     gene            complement(1386..>2776)
                     /locus_tag="VF870_03320"
     CDS             complement(1386..>2776)
                     /locus_tag="VF870_03320"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX9251242.1"
                     /translation="EMKVINTILLVVLVFVGCKAQQDQIVFKSNGKVDYPLSNSDEKL
                     LDSIQYRSFLFFKEQSNPENGLVKDRSASWAPASIAAIGFALPSYAVGVEKNWMSRDE
                     AAKITLKTLDFFLNSVQSPDTNATGYRGFYYHFLRMNSGTREWNCELSTVDTGLLMMG
                     IIFARNYYNLDNNVENEIRLAAEKLIDRLDWEFFIMPSNGKYANSISMGWDPKEGLHH
                     MGWVGYNEALFLYIIAAGSGMKNLSAAYNSWLDSYDWRTPYPGLSHAAFPPLFGHQFS
                     HTFIDFRGLVDSYMKEKGIDYFENSRRATLVQRQYAIDNPYKWVGYDSLCWGISASDG
                     PTEKYNFDDKKFLGYAGRGTSGPNFNYFDDGTIAPYAAISSIVFAPEVVLPTIKNFNK
                     KYGAGLWREYGYVDSFNPTLNWFNKEYIGIDQGPLLLMIENFRTGLIWNYVMKDPVIQ
                     KGLTRLGFEYIK"
BASE COUNT          887 a          540 c          417 g          932 t
ORIGIN      
        1 taaggacgaa ctaataaaaa tagcttgccc tctttttgtt ctttggagat atttgtaatt
       61 ctatacttta ggtaaagtgt agaattaata tttgcttcac catttgcaaa agcttcaact
      121 tcaagattta catcactgca attccaggtg acttttggta ttggcagata tccattttca
      181 agagattgtg ctgctgaaac attagcccag ttaaaataat cattacctat ttgtaacata
      241 ggttcaatag aaaaagctga tttatcgact tcaactattc catcttcatt aattaaagct
      301 tctttaacat cgttattaac accaattact gtccaaaaag aagcttcatc caaaaaatat
      361 cttgggtaat ctcctttaga ggaattcttt gctgtatata tcagaaaatc atttggggtt
      421 aatgaatttt ttatatccag aactttgatt tcgcttattc caaagttttc ctcagaatta
      481 ctttttaaca aatttattct taaaaatctt gattccgcct cagtgagttt aatgaaactt
      541 acattgcttc tatttgatga taccgagtaa actttctccc accttttacc atcgtctgaa
      601 agtaaaactt cgaatgaagc tgcttcataa ccttttaacc aatctatttt taatccaccg
      661 aattctcttc ttgttttaaa gtcgattgta atcgattgat ttttaacgcc gacacttttc
      721 caataagttt ctaatgaacc atcaacaaca ttttttggtt ccagattaaa gggctgcgat
      781 ttagcagtaa caaggggtag cgggtaatca taggtttcag gtggaagcgg ttcaaatttc
      841 agatcatcaa tccagatagt tcccttgcct ccaacaaaag atgcaatagt aaattcaatt
      901 ctatcaattc tctttaaggc ttggtcattt gtgggtcccc atgcaaagtt tatatgtctt
      961 tttttaactc taacctttct ccattccttc gggaaatcat agttgcgatt gtttacccac
     1021 caaacattat tgcctgtgct atcgataaat tttatttcaa aattgttagc cggagattct
     1081 gcttttagat aaaatgtaaa ttcataattt tcgggtacat caataggaaa gaatttttgt
     1141 attccgccat atccagttcc tttagtaaag ttataatcaa atctaattgc attcccattt
     1201 ataccagcat cagttgcaat attaagcgta actccatcag ctttaatttc actccatcca
     1261 ttgctgtttt caaaatcatc aagagtttta atctgtgcac tgttttgttc gttaaataag
     1321 ataagtacaa aaagcaggaa tggaaaatat ttttgaagca tgataattat caaagatatt
     1381 taatattatt taatatattc aaagcctaaa cgtgtaagtc ctttttgaat gaccgggtct
     1441 ttcatcacat aattccaaat caatccggtt cgaaaatttt caatcatcaa aagtaatggc
     1501 ccctgatcaa ttccaatata ttctttatta aaccaattta atgttgggtt gaaggaatca
     1561 acgtatccat attcccgcca taaacctgca ccatattttt tattaaaatt tttaattgtc
     1621 ggtaaaacaa cttcaggtgc aaatacaata gaggatattg ccgcataagg ggcaatagtt
     1681 ccatcatcaa aataattaaa attgggccca ctggttcctc ttccggcata acctaaaaac
     1741 tttttatcat caaaattata tttttcagtt ggtccatcgc tcgctgaaat accccagcac
     1801 aatgaatcat atccaaccca cttatatgga ttatcaattg catattgtct ttgaactaat
     1861 gtggctcttc gtgaattttc aaaatagtcg atcccctttt ctttcatgta gctatccacc
     1921 agaccacgaa aatcaataaa tgtatgagaa aactgatgac caaataatgg aggaaaagct
     1981 gcatgtgaaa gaccaggata tggagttctc caatcgtaag aatccaacca ggaattgtag
     2041 gctgcactga ggtttttcat tccgctgccg gcagcaatta tgtaaaggaa taatgcttcg
     2101 ttgtatccaa cccaacccat atgatgcagg ccctctttag ggtcccagcc cattgaaata
     2161 gagtttgcat atttaccgtt tgaaggcatt ataaaaaatt cccaatcaag cctgtcaatt
     2221 aatttttccg cagctaaacg aatttcattt tcaacattat tatcaagatt ataataattt
     2281 cttgcgaaga taattcccat cattaacaag cccgtatcaa cagtggaaag ttcacaattc
     2341 cattcgcgtg ttcctgagtt cattcttaaa aagtgataat aaaatcctct ataaccagtt
     2401 gcatttgtat caggactttg aactgagttt aaaaagaaat ccagggtctt aagagtaatc
     2461 tttgcggctt catcacgcga catccaattt ttttcaacac caacagcgta cgacggcaag
     2521 gcaaacccaa ttgctgcaat gctggcgggt gcccagcttg cacttctgtc ttttactaat
     2581 ccattttcag gattagactg ctctttaaag aaaagaaagg aacggtattg aattgaatct
     2641 aaaagttttt catcactatt tgagagagga taatcaactt ttccatttga tttaaagaca
     2701 atttgatctt gctgagcctt acatccaaca aacacaagca caacaaggag aattgtatta
     2761 attaccttca tttcaa
//
LOCUS       DASWBY010000185         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_685329, whole genome shotgun sequence.
ACCESSION   DASWBY010000185 DASWBY010000000
VERSION     DASWBY010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685329"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..694)
                     /locus_tag="VF870_03325"
     CDS             complement(110..694)
                     /locus_tag="VF870_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HEX9251243.1"
                     /translation="MKSDIILSGVGGQGILSIAAVVGMAALENHLYLKQAEVHGMSQR
                     GGAVQSHLRISEREIASDLIPEGRADIIISVEPMEALRYLPWLSDNGWLVTNTTPFNN
                     ISDYPDMKELLAEIEKLPNHISINADEIARQVKSPRSSNMVVLGAASPFIDIPYQSLE
                     NGIRKIFERKGEKIVQLNIDALKAGREFSEVHKK"
     gene            complement(691..2310)
                     /locus_tag="VF870_03330"
     CDS             complement(691..2310)
                     /locus_tag="VF870_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9251244.1"
                     /translation="MEKLLLLGAEAIAQGAIDGGMSGIYAYPGTPSTEITEYVQNNKI
                     ATERKLHSLWSANEKTAMEAGLGMSYAGKRAMVCMKHVGLNVAADAFINSAITGVNGG
                     LIVAVADDPSMHSSQNEQDSRFFGKFAMIPIFEPASQQEAYDMTYEGFELSEKLKIPI
                     MMRITTRLSHSRAGIETRPLKQENKLSYPEDPIQFVLLPSNARKRYKGLLGLQSLMEQ
                     ESERSSFNTFYPARNKSLGIIACGIAFNYLMENYPDRKFPHPVLQLGQYPLPFTKVEK
                     IVNECDQVLVLEDGYPLVEEMLKGYLEKDSKIKGRLDGTIPRDGELNPDIVAKALGLQ
                     VKIGKEVPTIVANRPPELCAGCSHRDIYNALNETMKEFGKQKVFSDIGCYTLGALPPW
                     NSINSCVDMGASITMAKGAADAGVHPSIAVIGDSTFTHSGMTALLDCVYENTPVTVII
                     SDNGTTGMTGGQASHATGRLEEICIGLGVKPEHVRTLTPLPKYHDEMVNVLREEMLYE
                     GVSVIIPRRECIQTLARSKKEIKVKVEEAVQ"
BASE COUNT          752 a          539 c          432 g          867 t
ORIGIN      
        1 ctatccatct agcaggaatg aattaaatat tatcatcata caacaagcaa gttttttctg
       61 ctgagacgaa gaattaatat ctctgcggct cagcagttaa atttttaatc tattttttat
      121 gaacttctga aaactctcta cccgctttta gtgcatctat gttaagctga actattttct
      181 ccccttttct ttcaaagatt tttctaattc cgttttcaag cgattgatat ggaatatcta
      241 taaatggaga agcagcacca agcactacca tatttgatga gcgcggagat tttacttgtc
      301 ttgcaatttc atcggcatta attgaaatat ggtttggcag tttttctatc tctgcaagaa
      361 gttctttcat atccggataa tctgaaatgt tattaaatgg agttgtgttc gtaaccagcc
      421 agccattatc tgaaagccat ggtaaataac gaagtgcttc catcggctca acagaaataa
      481 ttatatctgc tctgccttca ggaattaaat cacttgcaat ctctctttca gaaattctaa
      541 gatgagattg tacagcaccg ccacgctgac tcattccgtg cacttctgcc tgctttaagt
      601 aaagatgatt ttccaaagct gccattccaa caactgcagc gatagaaaga ataccttgtc
      661 cgccgacacc tgataaaatt atatctgatt tcattgtaca gcctcttcca ccttaacttt
      721 aatttccttc ttgcttctcg ctaaagtctg tatacattct cttcttggaa ttataacaga
      781 aacaccttcg tacaacattt cttctcgtag tacatttacc atctcatcgt ggtattttgg
      841 caatggagta agcgttctaa catgctcagg ttttacacca agaccgatac agatttcttc
      901 taaccttcct gttgcgtgag aagcctgacc acctgtcatt cctgttgttc cattatctga
      961 tataattact gtaaccggag tgttttcata aacacaatca agtagtgctg tcattccgct
     1021 gtgcgtaaaa gtagaatcac caataactgc aattgaagga tgaacgccag catcggcggc
     1081 acccttagcc atcgttattg atgcgcccat atcaacacag gaattaattg aattccaagg
     1141 aggcaatgcc ccaagtgtat aacatccaat atctgaaaaa actttttgct ttccgaattc
     1201 tttcatcgtt tcattaagtg cattgtaaat atctctatga ctgcagccgg cgcaaagttc
     1261 aggtggacgg tttgcaacaa ttgtagggac ttcttttccg atcttcactt gtaatcctaa
     1321 agccttggca acgatatcag gatttaattc gccatcccgt ggaatagttc catccaatct
     1381 tcctttaatc ttgctgtcct tttctaaata tccttttagc atttcttcaa ccaaaggata
     1441 tccgtcttca agaactaaaa cctggtcaca ttcatttaca attttttcaa ctttagtaaa
     1501 cggaagtgga tactgcccaa gctgcagaac agggtgagga aattttctat ccggataatt
     1561 ttccatcaaa taattgaatg caattccgca tgctataatt ccaagtgatt tatttcttgc
     1621 aggataaaat gtattgaaag atgatctttc agattcttgt tccattaaag attgcaaccc
     1681 aagcaatcct ttatatcttt ttcttgcgtt ggatggaagc agaacaaact gaataggatc
     1741 ttcaggataa cttaacttgt tttcctgctt taaaggacga gtttcgattc ccgcacgtga
     1801 gtgcgaaagc cttgtagtaa tgcgcatcat aattggaatc tttaattttt cagaaagctc
     1861 aaaaccttca taggtcatat cataagcttc ctgctggctt gccggttcga atatcggaat
     1921 catagcaaac ttgccaaaga aacgagagtc ctgttcattc tgtgatgagt gcattgatgg
     1981 atcatctgca acggcaacaa ttaatccgcc gttaactccg gttattgccg agtttatgaa
     2041 agcatctgcg gcaacattta atccaacatg cttcatacaa accattgccc gcttaccggc
     2101 gtaactcatt ccaagtcctg cttccattgc agtcttttcg ttagcagacc ataaactgtg
     2161 tagttttctt tctgttgcaa ttttattgtt ttgaacgtat tcggttattt ctgtggaagg
     2221 cgtaccggga tatgcataaa tgccagacat tcccccgtca atagcgcctt gtgctattgc
     2281 ttctgcaccg agtaataaaa gcttttccat ttttcctctc tttctgaatc acaatggaaa
     2341 tttctaaatt tttaataatc aaaaataagg aaatgatctc tgttttctaa ttaaaaccat
     2401 ttgtgatatt tgtaaaattt aaagttaatg ttaaaatttt ctcaggatac aactaatatt
     2461 agctgctatt cttatttttg attaacatat atttatttta caatatgatt attgaagaaa
     2521 ttggtaaaca gaaaactgta aatggggtta tacatctaac cgagccggat aagtcctttg
     2581 aagatttata
//
LOCUS       DASWBY010000186         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_308220, whole genome shotgun sequence.
ACCESSION   DASWBY010000186 DASWBY010000000
VERSION     DASWBY010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308220"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VF870_03335"
     CDS             complement(<1..1276)
                     /locus_tag="VF870_03335"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX9251245.1"
                     /translation="MELTQLLNSLHVIQVTGEVQRRDVADIVYDSRKVQKNSVFVAIK
                     GYKTDGHKFIQDAINKGAVAVVVEDDRVIPDELIAHSQIAKILVSDTRKALAELSKGF
                     YKDPTSRLKLIGITGTNGKTTSTFILKNILQSVGCKTGLVGTIANYIGELKVDSKLTT
                     PESNDLNKMFYNMIEAGCSHSVMEVSSHSLFLNRVYGLDFSAAIFSNITSDHLDFHNI
                     FEEYLKAKKILFDGLKSDSVAIINSDDESSTEIIKDCKAKIVTYGMADDTDYKIGNIR
                     YDLNGTEFTITYNNFEYSISTSLIGTFNAYNAASAFATAHSLGFDADKIVDGIQSSPQ
                     VPGRFEVLGNGNKKVIVDYSHTADSLEKALQAVREIVKDKNQVVTVFGCGGDRDKSKR
                     PVMGKVASDLSDNVIVTSDNPRTENPFEIINDI"
     gene            complement(1277..>2231)
                     /locus_tag="VF870_03340"
     CDS             complement(1277..>2231)
                     /locus_tag="VF870_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX9251246.1"
                     /translation="VAKLVQRIDDEKYFKYLRGFGFGNSTSLTLPGETAGRLRKPNEW
                     SKVSKTYLSFGYEISVTPVQIVSGYCALINGGVLYEPQLVQRQVSKAGKLVYEFSPKE
                     VRRVISSETSDTMRDLLQGVVKNGTGKKAYSELISIGGKTGTSQKLVDGSYSKQHYNS
                     SFVGFFPVENPKVVCLILINSPDQGKYGGLVAAPIFKNVAERIVQNDYQEFQEYINPD
                     LMKNLKFVEDNSANDYQDKTQINPINVKEVKLSSKNVMPDLINCQVKDAIYALTKLGV
                     KYKIKGTGIITSQSIAPGQKLNGKEVCVIECSEYMVRGASF"
BASE COUNT          696 a          440 c          298 g          797 t
ORIGIN      
        1 taatatcatt tataatctca aaagggtttt cagttcttgg attatccgat gtaactataa
       61 cattatcact caagtcgctc gcaactttac ccattacagg gcgtttagat ttatctctat
      121 caccaccaca accaaaaacg gttacaacct gatttttatc ttttacaatt tctctaactg
      181 cttgcaaagc tttctctaaa ctgtctgcag tgtgagaata gtctacaata acttttttat
      241 ttccattacc aagcacttca aatcttcccg gaacttgggg cgaggattgt atgccatcaa
      301 caattttatc agcatcaaag cctaagctgt gtgcagttgc aaaagcagat gctgcattgt
      361 aagcattaaa agtaccaatt aacgaagtgc taatactata ctcaaaatta ttgtacgtaa
      421 ttgtaaattc ggttccattc aaatcatacc tgatattacc gattttataa tcagtgtcat
      481 ctgccatccc ataagtaaca atttttgctt tacaatcttt tattatttct gtactgcttt
      541 catcatcgga attaataatt gcaaccgaat cagattttaa tccatcaaat aatattttct
      601 ttgctttaag gtattcttca aaaatattat gaaaatccaa atgatctgaa gttatgttag
      661 aaaatatcgc tgctgaaaaa tctaatccat ataccctgtt taagaataat gaatgagagg
      721 atacttccat aactgaatgc gaacaaccag cttcaatcat attgtaaaac attttgttca
      781 aatcatttga ttccggtgtt gttaattttg aatcaacctt taactcacca atgtagtttg
      841 ctattgttcc aactaatcct gttttgcagc caacactttg cagaatattt ttcaagataa
      901 atgttgatgt tgttttgcca tttgttcctg ttattccaat aagttttaac ctgctagtag
      961 gatctttata aaatcccttt gataattccg ccagagcttt tctagtatca ctcaccagaa
     1021 tttttgcaat ttgtgaatgt gctattaact catctggaat tacacgatca tcttctacta
     1081 caactgcaac tgccccttta tttatagcat cctgaatgaa cttatgccca tcagttttat
     1141 agccttttat cgcaacaaaa acagaatttt tttgaacttt tcttgaatca taaactatat
     1201 ccgcaacatc tcttctctga acttctccag taacctgaat tacatgcaaa ctattcagaa
     1261 gttgagttaa ttccatttaa aacgatgctc ctctaaccat gtattcagaa cactcaatca
     1321 cacaaacttc tttaccgttt aatttttgtc ccggagctat actttgtgaa gtaattattc
     1381 ctgttccttt tattttatat ttaactccaa gttttgtaag tgcatagatt gcatctttaa
     1441 cctgacaatt aataaggtcc ggcattacat ttttagaaga aagttttact tctttaacgt
     1501 taataggatt gatttgagtc ttgtcctgat aatcatttgc agaattgtct tctacaaatt
     1561 ttaaattttt catcaaatcc ggattaatgt attcctgaaa ttcttgatag tcattttgta
     1621 caattctttc agcaacattt ttaaatattg gtgcagcaac taatccgccg tacttacctt
     1681 gatctggaga attaattaat atcaaacaaa ccactttcgg attttcaaca ggaaaaaatc
     1741 caacgaagga ggaattgtaa tgctgcttag aataacttcc atcgactaat ttctgagaag
     1801 tgcctgtctt gcctccaata gaaattaatt ctgaataagc tttcttacct gtaccatttt
     1861 taacaacccc ttgcaaaaga tctctcattg tatcagaagt ttcagaagaa attactctcc
     1921 tgacttcttt aggagaaaat tcatatacca attttcctgc ctttgatact tgacgctgca
     1981 ctaattgtgg ttcatataaa accccgccgt taattaatgc acaataacct gatacaatct
     2041 gaactggtgt tacggaaatt tcatatccga acgaaagata tgttttagaa actttagacc
     2101 attcattagg ttttcttaat ctgccagcag tttcacccgg cagagttaat gatgttgaat
     2161 ttccaaatcc aaaacctctt aagtatttaa aatatttttc atcatcaatt ctttgaacaa
     2221 gttttgcaac g
//
LOCUS       DASWBY010000187         6415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_495502, whole genome shotgun sequence.
ACCESSION   DASWBY010000187 DASWBY010000000
VERSION     DASWBY010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495502"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /gene="rpoB"
                     /locus_tag="VF870_03345"
     CDS             <1..1158
                     /gene="rpoB"
                     /locus_tag="VF870_03345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854904.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9251247.1"
                     /translation="PPGLKGTVIKTRLFSRKKRDNDSKKLEKIAFDKLEQEFKNKLHD
                     HYQKLVEKLTRITKDQTTTGIRDLDGSVVLRSGSTIKDETFSGMEDVTKLDYTLDWFE
                     TKKTNNLINTLFRNYFTIKTDIEEDFKRERIKIQSGDELPPGIVQLAKVYVAKKRKLS
                     VGDKMAGRHGNKGVVAKIVPAADMPYLPDGTPVDIILNPLGVPSRMNLGQLYETALGW
                     VGKKLGVKFATPIFDGAKVEDVDEWLTKAGLETGSKTDLFDGRSGEKMHQKVTCGYIY
                     MLKLSHLVDDKIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEGYGASHILQE
                     ILTIKSDDVAGRAKVYEAIVKGENLQEPNIPESFNVLIKELQGLGLDIKIN"
     gene            1199..5458
                     /gene="rpoC"
                     /locus_tag="VF870_03350"
     CDS             1199..5458
                     /gene="rpoC"
                     /locus_tag="VF870_03350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX9251248.1"
                     /translation="MAFRVQENAMRDINSITIGLASPDDILSRSYGEVTKPETINYRS
                     FRPEKDGLFCEKIFGPTRDWECFCGKYKRIRYKGIICDRCGVEVTQKSVRRERMGHIG
                     LAVPVVHIWFFRTQPSKIGNIIGVSLKELEKIIYYESYVVLNPGITGLSRLDLISEDQ
                     YFEVMNSLPDGNEKLDNDDPKKFVAKIGGDAVKEILKKTNVEELSKQLREAVKVETSQ
                     QKKADFLKRLRVLEAFKEEEGKVPNRPEWMVLNYIPVIPPELRPLVPLEGGRFATSDL
                     NDLYRRVIIRNNRLKRLIDIKAPEVILRNEKRMLQEAVDALFDNSRRGSAVRSDNNRP
                     LKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKDMAVELFKPFI
                     IRKLIERGHNKTVKSARKVVDRKDPVIWDILAKIIEAHPILLNRAPTLHRLGIQAFQP
                     TLIDERAIRLHPMVCTAFNADFDGDQMAVHVPLSYEAQLEASLLMLSSHNILSPQNGS
                     PIVVPTQDIVLGCYYLTKLKEGDKGEGMMFSSPEEVIIAYDNRVVGLHAKIKVRIDGE
                     MIETTTGRVIFNQIVPKEMGYINQLLIKKVFGGIIGKIFIKLGNKVTAKFLDDLKDLG
                     FRYSTAGGLSVSFGDMIIPEEKQALITKANKKVEGILNEHEMGVITDAERYNKIIDVW
                     THTTNDVARALMEKIKQHDKGFNSLHMMVDSGARGSQEQVRQLAGMRGLMMKPQKTLS
                     GQAGEIIENPIVANFKEGLSVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVAQD
                     VIISEVDCGTILGIEIKALKDVEEEREPLAERITGRFAQEDVYDPRTEEVIIEAGNII
                     TEEIAERIADASIDSVYIRTVLTCESKRGVCLKCYGRNLTTGKPVEIGEAVGIIAAQS
                     IGEPGTQLTLRTFHLGGTSSRIASQSQVESNIDGIVKFEKVSSVEKTEKDPFGGADIK
                     INVVTGRRGLIEIYDENNRQLKKYDVPYGAELLVSDGQKIKKRQPLYNHDPYNAVILS
                     DIYGRVKFVDLVDGITLQQVTDDQTGHVQKVVIESKDKNLTPSILIENEDGVKKSFNL
                     PVRAYLSVEDGENILAGTILAKISKQTTKSRDITGGLPRVTELFEARSPHESAVVSEI
                     EGFVKFGARKKGSREILVSAPDKSDEKKYNVALQKHILVQEGDEIPAGEKITDGPVNP
                     HDILAIKGTSAVQEYLVNEIQDVYRLQGVKINDKHIEVIVRQMLQKIKIVSPGDTRFL
                     EEDIVDRIEFIEENNKISNMAYIEDKGDSKLKNGQLITKSKLREFNSDLKKKEKKEIV
                     ARNAEPATFEHILLGITQAALSTESFISAASFQETTKVLANAATEAKVDHLLGLKENV
                     VMGHLIPAGTGIRKYRNIILESEEAESAEEKVEEKELLSNE"
     gene            5611..5997
                     /gene="rpsL"
                     /locus_tag="VF870_03355"
     CDS             5611..5997
                     /gene="rpsL"
                     /locus_tag="VF870_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007070674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX9251249.1"
                     /translation="MPTINQLVRKGRVKILSKNKAPALDACPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTKTNVEVTAYIPGEGHNLQEHSIVMIRGGRVKDLPGVRYHIIRGT
                     LDTSGVADRKKSRSKYGTKKPKVNKK"
     gene            5994..>6415
                     /gene="rpsG"
                     /locus_tag="VF870_03360"
     CDS             5994..>6415
                     /gene="rpsG"
                     /locus_tag="VF870_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX9251250.1"
                     /translation="MRKRRAEKKYLKPDPKFNDVLVSKFVNCVMYDGKKAVARNVIYD
                     AFQLIEEKTSKPGLEVFKKAISNTQPMIEVRSRRVGGATYQVPTEVRPERRIALAMRW
                     IKNYSRSRGEKSMSAKLASELIAASNGEGSAVKKKEDV"
BASE COUNT         2300 a          940 c         1359 g         1816 t
ORIGIN      
        1 cctcctggac ttaaaggaac agttattaaa actcgtttat tcagtcgtaa gaaacgagat
       61 aatgattcaa agaaattaga aaaaatcgct tttgataaac ttgaacaaga attcaaaaat
      121 aagcttcatg atcattatca aaagttagtt gaaaaactta caaggattac aaaagatcag
      181 acaacaactg gaatcaggga tcttgatggt agtgttgttt taagaagtgg ttcaactatt
      241 aaagatgaaa cattctcagg aatggaagat gtaaccaaac tagattatac tcttgattgg
      301 tttgaaacta aaaaaacaaa taatcttatt aatacactct ttagaaatta tttcacaatc
      361 aaaacagata ttgaagaaga ttttaagaga gaaagaatta aaatacagag tggcgatgag
      421 ctgccccctg gaattgtgca gcttgcaaaa gtttatgtgg ctaagaagcg taaactttct
      481 gttggtgata aaatggcagg gcgacacgga aataaaggtg ttgtcgccaa gatagtgccc
      541 gctgcagata tgccttattt acctgatgga actcctgtgg atattatcct taatccgctt
      601 ggtgtacctt ctcgtatgaa ccttgggcag ttgtatgaaa cagctttagg ctgggtaggt
      661 aaaaaacttg gagtaaaatt cgcaactcca atttttgatg gcgcaaaagt agaagatgta
      721 gatgaatggt taacaaaggc cggattagaa accggtagta agacagactt gtttgatgga
      781 agatccggtg agaaaatgca tcagaaagtt acctgcggat acatttatat gttaaaatta
      841 agtcacttgg ttgatgataa gattcacgca aggtcaattg ggccatactc tcttattact
      901 cagcagccac taggcggtaa agcgcaattt ggcggccaga gatttggaga aatggaagtt
      961 tgggctctgg agggttacgg agcttctcac atccttcagg aaatcttaac aataaagagt
     1021 gatgacgttg ccggtagagc aaaagtttat gaagcaatag ttaagggtga aaacttacag
     1081 gaaccaaata ttcctgaatc gtttaacgtt ctaattaaag aactccaggg cttaggtctg
     1141 gatataaaaa taaattaacg ttaaacatta tttcacttag aaattaggag atataaaaat
     1201 ggcttttaga gttcaagaaa atgcaatgag agatattaac agtatcacca taggcttagc
     1261 aagtcctgat gatattttgt caagatcgta tggtgaagta actaaaccgg aaacaattaa
     1321 ttacagatcg ttccgtcctg aaaaagatgg attattctgt gagaaaatat ttggaccaac
     1381 gcgcgattgg gaatgctttt gtggtaagta caaaagaata agatacaaag gtattatctg
     1441 cgatcgatgc ggtgtggaag ttacgcagaa gagtgttaga cgtgaaagaa tgggacacat
     1501 tggacttgcc gttccagttg tacacatttg gttcttcagg acgcagccat ctaaaattgg
     1561 aaacataatt ggtgtcagtc ttaaagaact tgaaaaaata atttattatg aatcgtatgt
     1621 tgttttaaat cccggcataa ctggattaag cagattagat cttatttctg aagatcagta
     1681 ctttgaagtt atgaattcgc tacctgatgg taatgaaaaa ttagacaacg atgatccaaa
     1741 aaaattcgtt gctaaaattg gcggcgatgc agttaaagaa atccttaaga aaacaaatgt
     1801 tgaagaactt tctaagcagc ttagagaagc tgttaaagtt gaaacatcac agcagaaaaa
     1861 agctgacttc ttaaagagac tgcgcgtact tgaagcattt aaagaagaag aaggtaaagt
     1921 tcctaacaga cctgagtgga tggtattaaa ttatatccct gttattccac cagaactacg
     1981 cccgctcgtt ccacttgaag gtggaagatt tgcaacaagt gatctcaacg atttatatcg
     2041 tcgtgtaatt attcgtaata acagactaaa aagattaatt gatattaaag ctccggaagt
     2101 tattcttcgt aacgaaaaaa gaatgcttca ggaagccgta gatgcattgt ttgataactc
     2161 gagaagaggc agtgctgtaa gaagtgataa caaccgtcct ttaaaatcat taagcgatat
     2221 gcttaaagga aaacaaggaa gattccgtca gaatctttta ggaaagagag ttgattattc
     2281 aggtcgttct gtaattgttg ttggtccaga attaaaactt catcagtgcg gacttcctaa
     2341 agacatggct gttgaacttt tcaaaccttt tatcattaga aagctgattg aaagaggaca
     2401 taacaaaaca gttaagagtg caagaaaagt tgttgataga aaagatcctg ttatctggga
     2461 tattcttgct aagataattg aagctcatcc tattttgtta aaccgtgccc ctacgcttca
     2521 tagacttggt attcaagcat tccagcctac acttattgat gaaagagcta tcagacttca
     2581 tcctatggtt tgtactgcat ttaatgcgga ttttgatggt gatcagatgg cagtgcacgt
     2641 cccgctttct tatgaagcac agcttgaagc atcattgtta atgttatcaa gtcataatat
     2701 actttcacct caaaatggta gtcctatagt ggtacccaca caggatatag ttttgggttg
     2761 ctactatctt acaaaattaa aagaaggtga taaaggggaa ggaatgatgt tctcttcacc
     2821 cgaagaagtt ataattgctt atgataacag agttgttggt ttgcatgcta agataaaagt
     2881 tagaattgat ggagagatga ttgaaacaac taccggaaga gtaattttca atcaaatagt
     2941 acctaaggaa atgggttata tcaatcagct attaattaaa aaagtttttg gtggtataat
     3001 cggcaaaata tttattaagc ttggaaataa agttaccgcc aagttccttg atgatttaaa
     3061 agatttagga tttagatatt caacagccgg aggactttcc gtaagctttg gcgatatgat
     3121 aattcctgaa gagaagcaag cattaattac caaagctaac aaaaaagttg agggtatcct
     3181 taacgaacat gagatgggtg ttattacaga tgctgaacgt tacaataaaa tcattgacgt
     3241 ttggacacat acaaccaacg atgtggctcg tgctttaatg gaaaaaatta aacagcacga
     3301 taaaggtttt aattcacttc acatgatggt ggattcaggt gcaagaggtt ctcaagaaca
     3361 agtaagacag ttagccggaa tgagaggttt gatgatgaaa cctcaaaaaa ctctttctgg
     3421 tcaggcaggt gagattattg aaaacccgat tgttgcaaac tttaaagagg gactttcagt
     3481 tctagagtat tttatttcta cgcacggtgc tagaaaagga ttagctgata cagctttaaa
     3541 aactgcagat gctggatatc ttacaagaag gctagtggat gttgctcagg atgttattat
     3601 ttctgaagta gactgtggaa ctatacttgg tattgagatt aaagctctta aggatgttga
     3661 agaagaaaga gaaccattag cagagcgtat taccggacga tttgctcagg aagatgttta
     3721 tgatcctcgt acagaagaag tgataattga agcagggaac attataactg aagagattgc
     3781 tgaaagaatt gctgatgcca gtattgattc cgtatatatc aggacagtac ttacctgtga
     3841 atcaaaacga ggtgtatgtc taaaatgtta tggaagaaat cttacaaccg gaaaaccagt
     3901 tgaaattggt gaagctgttg gtattattgc tgcacaatcg attggtgagc caggaacaca
     3961 gcttactcta agaacattcc accttggtgg tacatcttca agaattgcat cacaatctca
     4021 agtagaatca aatattgatg gtatagtgaa gttcgaaaaa gtaagttctg ttgagaaaac
     4081 ggaaaaagat ccttttggtg gtgctgacat taaaattaat gttgttacag gtcgccgagg
     4141 attaattgag atctatgacg agaataacag gcaattaaaa aaatatgatg tgccttatgg
     4201 tgctgagtta ttggttagtg atggacaaaa aattaagaag agacaacctc tttacaatca
     4261 cgatccatat aatgcagtta ttctttctga catttatgga cgagtaaagt ttgtagatct
     4321 tgttgatgga ataactttac agcaagtaac tgatgatcaa acaggacacg tgcaaaaagt
     4381 ggttattgaa tcgaaagata aaaatttaac accaagtatt cttatcgaaa atgaagatgg
     4441 tgttaaaaaa tcatttaacc ttcctgtacg agcatattta tccgttgaag atggcgagaa
     4501 tattttagct ggaactattt tagcaaaaat atcaaaacaa actacaaaga gtagagatat
     4561 tacgggtggt cttccaagag taactgaact ttttgaagca agaagccctc atgaatctgc
     4621 agttgtttcg gagatagaag gttttgtaaa gtttggcgca cgtaaaaaag gttctcgtga
     4681 aattttagtt agcgctccag ataaatcgga tgagaaaaag tacaatgttg ctttacaaaa
     4741 acatatacta gttcaagagg gtgatgaaat tcctgctggt gagaaaatta cagacggtcc
     4801 tgtaaatccg catgatatat tagccatcaa aggaactagt gctgttcagg aatatcttgt
     4861 taatgaaatt caggatgttt acagacttca aggtgttaaa atcaatgaca aacatataga
     4921 agttattgta agacagatgt tacagaaaat aaagattgtt tctcctggag atacaagatt
     4981 ccttgaagaa gatattgttg accgaattga atttatcgaa gagaacaata aaatatctaa
     5041 tatggcctat attgaagata aaggcgattc caaactgaaa aatggtcagc tgattacaaa
     5101 atctaaatta agagaattca attctgatct gaagaagaaa gaaaagaagg aaatagttgc
     5161 tagaaatgca gaaccagcaa catttgagca tatactcctt gggatcactc aggcggcact
     5221 atcaactgaa agcttcatct ctgcagcatc cttccaggaa acgaccaagg tgcttgctaa
     5281 tgcggctaca gaagctaagg tagaccatct gttgggatta aaagaaaatg ttgttatggg
     5341 tcacttaatt ccggctggta caggaatcag aaagtataga aatataattt tagagagtga
     5401 agaagctgaa agtgcagaag aaaaagttga ggaaaaggaa ttattgtcga atgaatagta
     5461 attaaatcgg taatagttgc tgtttcttaa gctaattgat aataaaaaca gttaaaaata
     5521 aaaataattt cagttttctt gacaaaattt aaactgaaaa ctatatttga aatctgaatt
     5581 tttttaaaga agtaattttt aggagatttt gtgcccacga taaatcaact tgtgagaaaa
     5641 ggtagagtta aaattttatc aaaaaataaa gctccggctt tagatgcatg tcctcaaaag
     5701 agaggtgtgt gcacaagagt ttatacaact acacctaaaa aacctaattc tgctttaaga
     5761 aaagttgcca gagttagact tactaaaaca aatgttgaag taacagctta tattccaggc
     5821 gaaggacaca atttgcaaga gcactcaatt gtaatgatac gtggcggaag ggttaaagat
     5881 ttacctggag taagatacca cattattcgt ggtactcttg atacaagtgg agttgcagat
     5941 agaaagaaaa gtcgttcaaa atatggtaca aaaaaaccaa aagtgaataa aaaatgagaa
     6001 aaagaagagc agaaaaaaag tatttgaaac ctgatccgaa gtttaacgat gttttggtat
     6061 caaaatttgt taactgtgta atgtatgatg gaaaaaaagc cgttgcaaga aatgtaattt
     6121 atgatgcttt tcagttaata gaagaaaaaa cgagtaaacc agggttggaa gttttcaaaa
     6181 aagctatttc taatactcaa ccaatgattg aggttagaag ccgcagagtc ggcggtgcta
     6241 cttatcaggt tccaactgaa gttagacctg aaagaagaat tgcactagcg atgagatgga
     6301 taaaaaatta ttcacgatct cgtggcgaaa aatctatgtc tgcaaaatta gcttctgagt
     6361 taatagccgc ttctaatggt gaaggctctg cagttaagaa aaaagaagac gttca
//
LOCUS       DASWBY010000188         2903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_420473, whole genome shotgun sequence.
ACCESSION   DASWBY010000188 DASWBY010000000
VERSION     DASWBY010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420473"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VF870_03365"
     CDS             <1..485
                     /locus_tag="VF870_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9251251.1"
                     /translation="NSTNIEDLAETVNAMINRYDEPSTPEEVKEVKDTVKILLVEDYK
                     HSQIIVTRLLKKNNFESIVVVENGLQAYEQVQQQYYDLILMDMQMPVMNGFEATQKIR
                     ELEDYKETPIIALTAFAMKGDREKCLEAGATDYIPKPIDSQEFIEKVKYYTENKVIKK
                     "
     gene            571..2028
                     /gene="gltX"
                     /locus_tag="VF870_03370"
     CDS             571..2028
                     /gene="gltX"
                     /locus_tag="VF870_03370"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX9251252.1"
                     /translation="MINETPRVRFAPSPTGYLHVGGLRTALYNYLFAKRNNGTFVLRI
                     EDTDRNRYVEGAVENLIKALNWAGLNYDEGPEAGGNYGPYMQSERLDIYKKYADELIE
                     KGKAYYCFCSPERLKTLKEEQERQKLPQAKYDKHCLNLSKEEVERNLTANIPKVVRLN
                     VGQNQTIKFDDIIREHVEFESNNVDDQVLIKSDGYPTYHLANVVDDHLMKITHVIRGE
                     EWLSSTPKHVLLYDAFNWERPVFAHLPLLLNPDRSKLSKRQGDVAVEDYRDKGFLKDA
                     LINFVALLGWNAGDDKEFYYMDELINSFSLERVNKAGAVFDLQKLNWLNAEHLRKKTN
                     EELLNLLKDEIQKSKFKDQNFSDEYLILVIEAMKERVSFVKDFINTCLYFYEAPTEYE
                     QKSVEKNWKPETPEHLNKLKEEFEAIVNPTKENFEQALAKVSEQLNVGKGKLIHPLRL
                     AVSGQSTGPGMFDLLFILGKDEVIKRIETAIKKLN"
     gene            complement(2081..>2903)
                     /locus_tag="VF870_03375"
     CDS             complement(2081..>2903)
                     /locus_tag="VF870_03375"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560628.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HEX9251253.1"
                     /translation="WDDPRMPTISAYRRRGYTPEAIQNFAEIVGVAKRDAITDLALLE
                     FAIREDLNKKAQRVMAVLDPLKIVITNYDGKEELEAVNNPEDAAMGHRKIPFSKELYI
                     EKNDFMENPVKGFHRLIPGGEVRLRYAYIIKCDEVIKDENGEVVQLNCSYDVETKSGT
                     GTSTKKVKGTIHWVSVKDAIDCEVRLYDRLLTVEDPASEKDKDYKDLINPNSLEIISK
                     AKLEPFVKNSKPGDRFQFERQGYFCVDKKHTTKEKLVFNRTVTLKDTWAKTGGKK"
BASE COUNT          999 a          470 c          512 g          922 t
ORIGIN      
        1 aaaatagtac aaatattgaa gatcttgcag aaactgttaa tgcgatgatt aacagatacg
       61 atgaaccttc aactcctgaa gaagtaaaag aggttaagga tacagttaag atattattgg
      121 ttgaagatta taaacattct cagattatcg ttacccgact tcttaagaaa aataattttg
      181 aatcaattgt ggttgttgaa aacggtttgc aggcttatga gcaggtgcag cagcaatatt
      241 acgatcttat cttaatggat atgcagatgc ccgttatgaa tggtttcgaa gccacacaaa
      301 aaatccgtga gcttgaggat tacaaagaaa ctccaattat tgcactaaca gcttttgcaa
      361 tgaaaggtga tcgtgaaaag tgtctggaag ccggagcaac tgattatatt ccaaaaccaa
      421 ttgacagtca ggagtttata gaaaaagtta aatactacac agagaataaa gttattaaaa
      481 agtagtgtct gctattctcc aatctatcct gttcattcat acaaatacat ttttgtattt
      541 ttatacattc aattttagga tatccaaaaa atgattaatg aaactccacg cgttcgtttt
      601 gcacccagcc caacaggtta tcttcatgtt ggcggcttgc ggactgcatt gtacaactat
      661 ctttttgcaa agagaaataa cggcactttt gttttaagaa ttgaagacac cgatcgtaac
      721 agatatgttg aaggagcagt tgagaattta attaaggcac taaattgggc tggattaaat
      781 tatgatgagg gtcctgaagc aggaggaaat tatggaccat atatgcaatc tgagagatta
      841 gatatttata aaaaatatgc tgacgagctg attgaaaaag gtaaagcata ttattgtttt
      901 tgctctccag aaagattaaa aactttaaaa gaagaacagg aaagacaaaa acttcctcaa
      961 gcaaaatatg acaagcattg tctgaatctt tcaaaagaag aggttgaaag aaatttgaca
     1021 gcaaatattc ccaaagtagt aagattaaat gtcgggcaaa atcaaactat taagtttgat
     1081 gatataattc gtgaacatgt tgagtttgaa agtaataatg ttgatgatca ggtattaatt
     1141 aaaagtgatg ggtatccaac ttaccattta gcaaatgtag ttgacgatca tttaatgaaa
     1201 ataactcatg tcatccgagg tgaagaatgg ttatcatcaa caccaaaaca tgttttactt
     1261 tacgatgcat ttaattggga acgacctgtg tttgctcatc ttccgctcct tttaaatcca
     1321 gatagatcaa aactttctaa acggcagggt gatgtagccg ttgaagatta tcgtgataaa
     1381 ggatttttga aagatgcact tataaatttt gtagctcttt taggatggaa cgctggtgat
     1441 gataaagagt tttattatat ggatgaactg ataaatagtt tttctttgga aagagttaat
     1501 aaagcaggag ctgtatttga tctccagaaa ttaaactggt tgaatgcaga acacttgaga
     1561 aagaagacaa atgaggaatt gttaaacctt ttgaaagatg aaattcaaaa gtccaaattc
     1621 aaagatcaaa atttctctga tgaatattta attttagtaa ttgaagcaat gaaagagcgg
     1681 gtttcatttg taaaagattt tattaatacc tgcttgtatt tttatgaagc accaactgag
     1741 tatgaacaaa aatccgttga gaaaaactgg aaacctgaaa ctccggaaca tttaaataaa
     1801 cttaaagaag aatttgaagc aatagtaaat ccaaccaaag aaaattttga acaggcactc
     1861 gcaaaagttt ctgagcaatt aaatgtcgga aaaggaaagc ttattcaccc tttaagatta
     1921 gccgtttcgg gccaaagcac cggaccagga atgtttgatt tactttttat tcttgggaaa
     1981 gatgaagtaa ttaaaagaat tgaaacagca attaaaaaat taaattaagt taaaaacact
     2041 gtcacactct aattctatga gtgtgacagc attaaaaata ttatttcttc ccgccggttt
     2101 tagcccatgt atcttttaaa gtaacagttc gattgaaaac tagtttttct ttcgttgtat
     2161 gttttttatc tacgcagaaa tatccttgtc tttcaaactg aaaacgatca cctggttttg
     2221 agttttttac aaatggctca agctttgctt tagaaattat ttcgagtgag tttggattaa
     2281 ttaaatcttt ataatcctta tccttttcac ttgcgggatc ctcaacagtt aataatctgt
     2341 cgtacaatct aacttcgcaa tcaattgcat ctttaacaga aacccaatgg attgttcctt
     2401 taactttttt agtacttgtt cctgttccgc tttttgtttc aacatcatag ctgcaattga
     2461 gctgtaccac ttccccattt tcatccttaa tgacttcatc acatttaatt atgtaggcat
     2521 aacgcaacct aacctctcca ccgggaatca atcgatgaaa tccttttaca ggattttcca
     2581 taaaatcatt tttctcaata taaagttctt ttgagaatgg aattttacga tgacccatcg
     2641 cagcatcttc aggattatta accgcttcaa gttcctcttt accatcgtaa ttggttatta
     2701 caatttttaa gggatctaga actgccataa ctctttgcgc ttttttattc aggtcttccc
     2761 ggatagcaaa ttcaagtaaa gcaagatcag taattgcatc acgtttagca actccaacaa
     2821 tctcagcaaa attctgaatt gcctctggag tatatcctct tcttcggtaa gcagaaatag
     2881 ttggcatacg aggatcatcc cat
//
LOCUS       DASWBY010000189         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_81958, whole genome shotgun sequence.
ACCESSION   DASWBY010000189 DASWBY010000000
VERSION     DASWBY010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81958"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..635)
                     /locus_tag="VF870_03380"
     CDS             complement(123..635)
                     /locus_tag="VF870_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251254.1"
                     /translation="MDEFYLIAKILSAGKEGFVKVQFVPGLNINLDSIKFLYLDFWEQ
                     KKKIELEEILNFKQSVFFKFKNFDDERDVSVLIDRKIFISVNDFEKVVVNVSLDQNII
                     GSKVYKGQDFLGIVSDYFETPANPVIEIKRDGRNNLLIPYVVSVFEKIDLENKVLILN
                     SDFGIDDDED"
     gene            complement(653..886)
                     /locus_tag="VF870_03385"
     CDS             complement(653..886)
                     /locus_tag="VF870_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX9251255.1"
                     /translation="MKEFVEFIAKQLVDNPDGVVVEEKLAEEKKVVLSLKVDANDIGK
                     VIGKQGKTAQAIRTLLTAVSAKEGKRAVLEIED"
     gene            complement(1042..1431)
                     /gene="rpsP"
                     /locus_tag="VF870_03390"
     CDS             complement(1042..1431)
                     /gene="rpsP"
                     /locus_tag="VF870_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX9251256.1"
                     /translation="MAVKLRLRRMGKKKQPIYKVVAADARSPRDGKFLEAVGIYNPLT
                     NPHTIDLKEDRINYWLDNGAQPTDTVNSLLKQKDINLKRDIAKRKLPQEKAEALLKSW
                     EANKEASAQKKSVKKKKKADTQEAKAE"
     gene            complement(1463..2677)
                     /gene="ffh"
                     /locus_tag="VF870_03395"
     CDS             complement(1463..2677)
                     /gene="ffh"
                     /locus_tag="VF870_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX9251257.1"
                     /translation="MLLDADVNYKVAKDFIENVKVKALGKEVIASISPGQLITKIIYD
                     ELTELLGGGYQELQINPSGITTILVVGLQGSGKTTFCGKLAKKLTEQKRRVLLVAADI
                     YRPAAIEQLKTLGKQIDIEVFSIEEKDARKVAAESLIYAKEKNYNTIIFDTAGRLHVD
                     EQMMLEVSDIKGIVNPNESLFVVDSMTGQDAVNSAKAFNETVNFDGIVLTKLDGDSRG
                     GAALSIRSVVGKPIKFISNGEKLENLEIFYPDRLASRILGKGDVISLVEKAQASFEDF
                     ETEELEEKLRKNKFDFDDFLKQIKVIKKMGSLSSLLGMIPGLGAKIKNAPIDDNALVK
                     VEAIINSMTKEERSSPKVLNGNRRKRIARGSGTSIQDVNRLVKQFSEMQRMMSSFSKK
                     GLPNSMRNFKFN"
     gene            complement(2656..2784)
                     /locus_tag="VF870_03400"
     CDS             complement(2656..2784)
                     /locus_tag="VF870_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX9251258.1"
                     /translation="MFEELSLKLESALKKVTGQGKLTEANISDTLREIEEFFLTRM"
     gene            complement(2818..>3662)
                     /gene="atpG"
                     /locus_tag="VF870_03405"
     CDS             complement(2818..>3662)
                     /gene="atpG"
                     /locus_tag="VF870_03405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX9251259.1"
                     /translation="GVKSTQQITKAMKMVAAAKLRRATESILNARPYAKKISVLLSHL
                     VNEDDKNSNPLFAEREVKNVAVVVVTADRGLCGAFNTNIIKEASRYVEEEIKQKGLDY
                     QLYCLGKKGSDYFSRRNYNIAATYPGIFSHIDYTTAQKLVDEIIPKYLDGSIDKVIIV
                     FNEFKSIIQQKIVVEQFLPIASGDEKEDDKHESANYIYEPDQKAIFNYLIPKHLKGQM
                     WRVLLESNASEFAARMTAMDNATTNAKELIRTLSLTYNSKRQAAITTEILEIVSGANA
                     LKEN"
BASE COUNT         1089 a          733 c          514 g         1326 t
ORIGIN      
        1 tattaaatgc ataatcgcga agattatgaa tttcaatttt aacttttttc ttgtcctgcg
       61 ctcttttaag aatgcttgta ttaagcggac ttgagaggag ctcaggtact gcagaaataa
      121 tatcaatctt catcatcatc aataccaaag tcagaattta aaatcaaaac tttattttca
      181 aggtcaattt tttcaaaaac ggagacaaca taaggaatca gcaaattatt tctaccatct
      241 cgttttattt caataaccgg atttgccgga gtttcaaaat aatcactaac aattcctaaa
      301 aaatcctggc ctttgtaaac tttactccca attatattct gatcaaggga aacattaaca
      361 acaactttct caaaatcatt aacacttata aagatctttc tgtcaattag taccgaaaca
      421 tctctttcat catcaaaatt tttgaactta aaaaaaactg attgtttaaa gttcaatatt
      481 tcttcaagtt caatcttttt cttttgttcc caaaaatcca gatataaaaa cttaatggaa
      541 tcaagattaa tatttagtcc tggaacaaac tgaactttta caaatccttc tttgccagca
      601 gaaagaattt ttgctattag atagaattca tccaattaaa atacttgaat gattaatctt
      661 caatttcaag aacggctcgc ttgccttctt ttgctgaaac agctgttaaa agtgtacgga
      721 tagcctgagc agttttgcct tgcttgccta tcactttgcc aatatcatta gcatcaactt
      781 tcaaagataa aaccactttt ttttcttcag ctaatttctc ttcaactaca acaccatctg
      841 gattgtcaac cagctgtttt gcaatgaact caacgaactc tttcataata gaagcctcct
      901 ataatgaacc cgggttaata gtttgttatg tacagtcata ataaactttg aagtttatta
      961 ttcacaattc agaagcggaa cccgtaactt ctgccgtacc tccattccat aatcaaagac
     1021 aaaaaagtaa ttgagaagtt gttattcagc tttagcttct tgagtatctg cttttttctt
     1081 tttcttcaca ctttttttct gtgcagaagc ttctttatta gcttcccagc tttttaaaag
     1141 tgcttcggct ttttcctgtg ggagtttacg ttttgcgata tctctcttaa ggtttatgtc
     1201 cttctgtttt agcaaactgt ttactgtatc agttggctgt gcaccgttgt ctagccagta
     1261 atttattcta tcttctttca aatctattgt gtgtggattt gtaagtggat tataaatgcc
     1321 aaccgcctca agaaatttac catctcttgg tgagcgtgca tctgcagcaa caactttgta
     1381 aatgggttgt ttcttttttc ccattcttcg taatcttaac ttaacggcca aattattatc
     1441 ctccggatat tatctctatt tttcaattaa atttaaaatt gcgcattgaa tttggtaatc
     1501 cttttttaga aaaactactc atcattcttt gcatttcact aaactgctta actaaacggt
     1561 ttacatcctg aattgatgta ccacttcccc ttgctattct tttacggcga ttgccgttca
     1621 aaaccttggg tgaacttctt tcttccttcg tcattgaatt gataattgct tcaactttaa
     1681 ccagtgcatt atcatcaatt ggtgcatttt ttattttagc accaagaccc ggaatcattc
     1741 caagtaagga agataaagaa cccattttct tgatgacttt aatttgctta agaaaatcat
     1801 caaaatcaaa tttatttttt cttagttttt cttctaattc ttcagtttca aagtcttcaa
     1861 atgatgcctg agccttttca acaagggata ttacatcacc tttacccaat atcctggatg
     1921 ccaatcggtc cggatagaat atttctaaat tctccaattt ttcaccatta ctgataaact
     1981 taatgggttt accaacaacg gatcttattg aaagagcagc gccacctctt gaatcaccat
     2041 ccagttttgt aagaacaata ccatcaaaat ttacagtttc attaaaagct tttgcagaat
     2101 taactgcatc ctgccctgtc atcgaatcaa caacaaacaa actctcgtta ggatttacaa
     2161 tgcctttaat gtctgaaact tcaagcatca tttgttcatc aacatgaagt ctgcctgctg
     2221 tatcaaatat tatagtattg taatttttct ctttagcata gattaaggat tcagctgcta
     2281 cttttcgagc atctttttct tcaatagaaa aaacttcaat atcaatctgc ttccccagcg
     2341 ttttcaattg ctcaatagcc gcaggtctgt atatatcagc tgcaactaac aagactcttc
     2401 ttttttgctc agttaatttt tttgcaagtt taccgcaaaa agttgttttc cctgaacctt
     2461 gcaaacctac aaccagaata gttgtaatac cagaaggatt tatctgcaat tcctggtaac
     2521 caccgccaag taactcagtt aattcatcat aaataatttt agtgattaac tggcccggtg
     2581 aaattgaagc aattacttcc ttaccaagtg ccttaacttt tacattctca ataaagtctt
     2641 ttgcaacttt gtaatttaca tccgcgtcaa gaagaattct tctatttctc gtaaagtgtc
     2701 ggaaatgtta gcttcggtta gcttaccttg accggtaact tttttaagag cagattctaa
     2761 ctttagagat aattcttcaa acataatttc aacagaccaa ctttactaaa accaaatcta
     2821 attttctttt aaagcatttg caccggacac gatttctagt atctctgtgg taattgctgc
     2881 ttgtcgtttg ctgttataag taaggctaag tgttcttatt aattcttttg catttgtagt
     2941 tgcattatcc attgctgtca tacgtgccgc aaattcagat gcgtttgatt ccaacaaaac
     3001 tctccacatc tgtcctttaa gatgttttgg aatcaaataa ttgaaaattg ctttttgatc
     3061 cggttcataa atataatttg cagattcgtg cttatcatct tccttttcat caccggatgc
     3121 aattggtaaa aactgttcta caacaatttt ttgctgtatg atcgatttaa actcattaaa
     3181 aacaattatt actttatcaa tacttccatc aagatatttg ggaataattt catcaactaa
     3241 tttttgtgct gttgtataat ctatgtggga aaatattcct gggtaagtag cagcaatgtt
     3301 ataatttctc cggctaaaat aatctgagcc tttcttaccc aaacaataaa gttgatagtc
     3361 taaacctttt tgcttgattt cttcttcaac atatcgtgac gcttctttaa taatgtttgt
     3421 attaaaagct ccgcaaagcc cgcgatcagc tgtaactaca acaactgcaa cattcttcac
     3481 ttctctctca gcaaacaaag gattggaatt tttatcatct tcatttacaa gatgtgatag
     3541 caatacagat atttttttgg cataagggcg ggcattcagt attgattcag tagcacggcg
     3601 tagctttgcc gcagcgacca tcttcatcgc ttttgtaatc tgttgcgtgc tttttacacc
     3661 tt
//
LOCUS       DASWBY010000190         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_495522, whole genome shotgun sequence.
ACCESSION   DASWBY010000190 DASWBY010000000
VERSION     DASWBY010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495522"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="VF870_03410"
     CDS             complement(<1..1335)
                     /locus_tag="VF870_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEX9251260.1"
                     /translation="MKEKSRTPVSQQPLTIEKPTLEDGIGSKVTVEINEKKVQVFFGQ
                     TILEACKENKIHVPTLCHHPDLCIAGTCRICVVEIEGMRTLQTACSFPITAPIKIKTS
                     SSMVRKARRHIIDLLLSEHYGECYSCVRNNNCELQSLANEYGVDNYTFGHIKEPIFEE
                     DLSSYAVVRDMNKCINCRRCVRTCIDLQEVGVLEAIDRGDKTHIGTFLEKPLADVICI
                     NCGQCINRCPTGALKANDPSDVIWSAIDDPTKHVVIQTAPSPRAAIGEVFGLEPGKSF
                     TGEMNTALRRIGFDVVFDTNFTADLTIMEEGTELILRLHKALVKKEQVAIPQFTSCSP
                     GWVKYLEHFYPEYIDNLSSAKSPQQMFGALIKTFYAQKKGIDPKNIVSVAVMPCAAKK
                     FECNRPEMNDSGFKDVDYGLTTRELGQMIKEAGIYLPEMPKSHFDDPFGEASG"
     gene            complement(1335..2570)
                     /locus_tag="VF870_03415"
     CDS             complement(1335..2570)
                     /locus_tag="VF870_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX9251261.1"
                     /translation="MKTQTEMNIIKTERTGPVIFSVYKRGDGVKKALKQKREDILFEL
                     KDSGLKGRGGAGFPTSTKWMLTAAAKAEKKFIVCNADEGEPGTFKDRVLLMEYPQLVF
                     EGMIIGGYTVGANVGIVYLRAEYEYMLEQLENYLSNMREDNLLGSGILGTDFSFDISI
                     RLGSGAYVCGEETALIESLEGNRGEPRNRPPFPVNTGYMGYPTSVNNVETLAAVPHII
                     MKGGGWFKKFGTDRSTGSKLFSVSGDCEKPGVYELPWGISINELLEIVEARNTKAVQV
                     GGASGTCIPKSQFDRKFGYEDIPTGGSVIIFNESRNMLHVLKNFMEFFAEESCGQCTP
                     CRIGNVKLLEGINMIERGEFTFKYINTLKELGKTMQVASKCGLGQSSPNAFISILENF
                     KDEIFHTSGNDGYNGKAGM"
     gene            complement(2579..3052)
                     /gene="nuoE"
                     /locus_tag="VF870_03420"
     CDS             complement(2579..3052)
                     /gene="nuoE"
                     /locus_tag="VF870_03420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX9251262.1"
                     /translation="MLVVEKNALSEEIEGYVIKYGKDRSSLLPVLQAIQKKHSYISDF
                     AQQEVARLLDIHPVEVFSVISFYSFLHSKPTGRNIVRLCQTISCDMANKSKIVQAVER
                     ELGIKFGETTKDNRITLEYTNCVGMCDQGPAMIINDRVYTKLDPEKAVNILNEIK"
     gene            complement(3057..3437)
                     /locus_tag="VF870_03425"
     CDS             complement(3057..3437)
                     /locus_tag="VF870_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9251263.1"
                     /translation="MAKIAIIDDDPDIVDASSLVLQSKGYDVITASNPTDGYKIVMEQ
                     NPDLIILDVMMIEPDDGFFLAQKFRKMNVDVPIIMYTSVSKSLGMEFGVSEMVPVDEF
                     VEKPISPETLIDKVEKLLAIHFKE"
     gene            4064..>4426
                     /locus_tag="VF870_03430"
     CDS             4064..>4426
                     /locus_tag="VF870_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX9251264.1"
                     /translation="MIIDSSRPHIAVVGRRNVGKSLLVNSLLEQNVTTVSDIAGTTTD
                     AIKTKYELLPYGPVVIVDTAGIDDEGELGRQRITETIKILSSSDFALVVLDARETLQN
                     KELELFAYLDRLQINYIVA"
BASE COUNT         1331 a          864 c          717 g         1514 t
ORIGIN      
        1 cccagatgct tctccaaaag gatcatcaaa atgtgattta ggcatttcag gtaaatatat
       61 tccggcttcc ttaatcattt gcccgagttc tcgagttgtt aatccgtaat caacatcttt
      121 aaagcctgaa tcattcattt caggacgatt gcattcaaac tttttagctg cacaaggcat
      181 tactgcaact gatactatat ttttaggatc aatgcctttt ttctgcgcat aaaacgtttt
      241 gatcaatgca ccgaacattt gctgtggtga ttttgcagaa cttaagttat caatgtactc
      301 gggatagaaa tgctcaagat attttaccca gcctggtgag catgaagtga actgcggaat
      361 tgcaacctgt tccttcttaa ccaatgcttt gtgaagcctt aaaattaatt ctgtaccttc
      421 ttccattatt gtaagatccg ctgtaaagtt tgtatcgaag acaacatcaa aaccaattct
      481 gcgtaaagcc gtattcattt cacctgtaaa agattttcct ggttctaaac caaacacttc
      541 accaattgca gcacgcggcg atggagccgt ttgaattaca acatgttttg ttggatcatc
      601 aattgcactc cagatcacat ctgatggatc atttgctttt aatgcgccag ttgggcatcg
      661 atttatacac tgaccgcagt taatacaaat aacatctgcc aacggttttt ctaaaaacgt
      721 tccaatatgc gttttatctc cgcgatcaat tgcttccagt acgccaactt cctgaaggtc
      781 gatacatgtt cttacgcatc ttcggcagtt gatacatttg ttcatatcac gaacaacagc
      841 ataagaagaa aggtcctctt caaaaatcgg ttctttgatg tgaccaaatg tataattatc
      901 tactccatat tcattagcta atgattgaag ttcgcaatta ttatttctta cacaggaata
      961 acattcaccg taatgttcac taagcaaaag atcgataata tgtcttcttg ctttgcgaac
     1021 catactgctt gaagttttaa tttttatagg agcagttatc ggaaatgaac aagctgtttg
     1081 cagagttctc attccttcaa tttcaacaac acaaatacga caagtgccgg caatgcaaag
     1141 atcgggatgg tgacaaagtg ttggaacgtg aattttattt tctttacaag cttcaagaat
     1201 tgtctgtcca aaaaatacct gaactttttt ctcatttatt tcaacagtta ctttacttcc
     1261 aattccatct tctaaagttg gtttctctat tgtaagtggt tgttgagata ctggagttct
     1321 tgatttttct ttcatcacat acctgccttt ccgttatatc cgtcatttcc tgaggtatga
     1381 aagatttcat ccttaaaatt ttctaatatg gatatgaacg catttggact ggattgaccc
     1441 aatccgcatt ttgaagctac ctgcattgtt tttccaagtt ctttaagagt attaatatat
     1501 ttaaaagtaa attctccgcg ttcaatcata ttaattcctt ccagaagttt tacgttaccg
     1561 attctgcaag gtgtgcattg cccgcatgat tcctcagcaa aaaactccat aaagttttta
     1621 agaacgtgaa gcatatttct tgattcatta aaaataatta ctgatcctcc ggtaggaata
     1681 tcttcataac caaattttcg atcgaactgt gatttaggaa tacaggtacc cgatgcaccg
     1741 ccaacctgaa cagcttttgt gtttcttgct tcaacaattt caagcaattc attgatactg
     1801 attccccagg gcaattcata aacgcctggt ttttcacaat caccagaaac agaaaataac
     1861 ttagaacccg tcgatctatc agttccaaat ttcttaaacc atccgccgcc tttcataata
     1921 atatgaggaa cagcagcgag tgtttcaaca ttatttacag atgttgggta acccatataa
     1981 cctgtattta caggaaacgg cggtctgtta cgcggttcac ctctgttgcc ttcaagtgat
     2041 tctataagtg cagtttcttc tccgcatacg taagcacctg aaccaagacg aatagaaata
     2101 tcaaaagaaa aatctgttcc aagaattcct gatccgagca gattgtcttc tctcatattc
     2161 gaaagataat tttccaactg ctcaagcata tattcatatt ctgcgcgcag ataaactatc
     2221 cctacattcg ctccaacagt atatccgcct ataatcattc cctcaaaaac taattgcgga
     2281 tattccatta aaagcactct gtctttaaat gtgcctggtt cgccttcatc agcgttacag
     2341 acaataaatt tcttttctgc tttggctgcg gcagttaaca tccattttgt agatgtcgga
     2401 aaaccggcgc cgcctctccc ttttaatcct gaatctttaa gttcaaaaag aatgtcttct
     2461 cttttttgct ttaatgcttt cttaactcca tctcctcttt tgtaaacgga gaatataaca
     2521 ggacctgttc tttctgtttt tattatgttc atttcagttt gtgttttcat ttctccccct
     2581 acttaatttc gttaagaatg ttaactgctt tttcgggatc gagctttgtg taaacacgat
     2641 cattaattat cattgctggt ccctgatcac acataccaac acagtttgta tactcaagag
     2701 taatacgatt atcttttgtt gtttcaccaa actttattcc aagttctctt tcaacggcct
     2761 gaacaatttt acttttgttc gccatatcgc aggagatagt ctggcaaagt cttacaatat
     2821 ttcttccggt aggttttgag tgaaggaaag aataaaaaga tattacgctg aacacttcta
     2881 cgggatgaat atcaagcaag cgggcaactt cctgctgcgc aaaatcgctt atataactat
     2941 gcttcttttg tattgcctga agtaccggca acagagatga tctgtcttta ccatatttta
     3001 ttacgtagcc ctcaatctct tcagagagag catttttttc tactacaagc atttgctcac
     3061 tccttaaaat gaattgctaa aagtttttct actttatcta ttaatgtttc aggtgaaatt
     3121 ggtttttcaa caaactcatc taccggaacc atttcgctga cgccaaattc cattcccaaa
     3181 gattttgata ctgatgtata cattatgatc ggtacatcaa cattcatctt gcggaatttt
     3241 tgagcaagaa aaaatccatc gtccggttca atcatcatta catcaagaat aattaagtcc
     3301 ggattttgct ccataacgat tttgtaacca tcggttggat tagaagctgt gataacgtcg
     3361 taccctttgg attgtaatac caaactgctt gcatctacaa tatcaggatc gtcgtctatg
     3421 attgctattt tagccatgtt tatcccctaa agataaattt atggaatatg tagttaatta
     3481 agtgttttca agtaatcatc aattaacatg ttaagtgcca tattacttta caacaaataa
     3541 ttattttaat tcatttagtg tgatgataaa agtctgttta gttttatttt tcttaaacct
     3601 aagaagacat tcttctcaaa taagatatgc tgtttattag cttttgctaa atattaaatg
     3661 atgttaaatg ggaattgaat tgcgcgatga tgaataaaaa cttaaaaagt ttttaaagag
     3721 tgtctcatca attgcgttta atatggcaat gaattttcct gatatgtttt gctgtttact
     3781 tattaaagaa cgtaaatcag atctgattat atgagcacaa atcaaaagca gccctatcat
     3841 tccaagacta aaatattctt gattcagaga aatatatttt aaaacttgaa attattacac
     3901 aattgtgaaa taatattcat caataaaaac aaatatatct ttcctttaag gggattaatt
     3961 tagtggaaca ttttttgacc ttctggtttt tcagggaagt gatatttaca taataaattt
     4021 aagtaattat caagtttagt agttttggaa agtttaatct tttatgatca tcgattcaag
     4081 ccgtccgcat atagctgttg ttggtagaag aaatgtaggc aaatctttgc tcgtaaattc
     4141 gttactcgag caaaatgtaa ccacggtttc cgatattgca gggacaacaa cggatgcaat
     4201 aaaaaccaag tatgaacttt taccttatgg tccggttgta attgtagata ctgcaggtat
     4261 tgatgatgaa ggcgaacttg gaagacagcg cattactgaa acaataaaaa ttttatcatc
     4321 ttctgatttt gctttggttg tacttgatgc ccgcgaaact ttacaaaaca aggagctcga
     4381 gctgtttgca tatctggata gacttcaaat taattacata gttgct
//
LOCUS       DASWBY010000191         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_232636, whole genome shotgun sequence.
ACCESSION   DASWBY010000191 DASWBY010000000
VERSION     DASWBY010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232636"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /gene="ugpC"
                     /locus_tag="VF870_03435"
     CDS             complement(<1..1117)
                     /gene="ugpC"
                     /locus_tag="VF870_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX9251265.1"
                     /translation="MAEVLLKNVTKIYDGGNKVVTNANFKVEDKEFVVLVGPSGCGKT
                     TTLRMIAGLEEISGGEIFIDGKLVNNLPPKDRDIAMVFQNYALYPHMTVYENMAFGLK
                     LKKVDKKEIDVRVKEAARILGLETYLDRKPKALSGGQRQRVAVGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRTEISKLHKQLGATMIYVTHDQTEAMTMGDRIVIMKDGIINQVDT
                     PLNLYNKPVNQFVAGFIGSPAMNFLSGKISEKNGLMFESNNSEFHFHLTDRQKDYLKN
                     STVEEITIGIRPEDISVEKSSEKSIELESVLDVIEPMGNESFIYFEVEKVQFIARVKS
                     LQDLKAGEKIKLYLDPEKIYLFDKIKGINIIGVLGLNK"
     gene            complement(1127..>1515)
                     /locus_tag="VF870_03440"
     CDS             complement(1127..>1515)
                     /locus_tag="VF870_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251266.1"
                     /translation="IGRIAQGLNIGQVYGPMKVNDGYSIFKLIEKKNRNDKQKSIIDQ
                     DSLKFIRLKTALTKMDNLINDKTVSLARKYKINLDEQLLQKIESSEINTFTYRFIGFG
                     GKIAAFPITVPMYKWYKQYEQNNEIP"
BASE COUNT          414 a          309 c          203 g          589 t
ORIGIN      
        1 atttattaag tcccaataca ccgataatat ttattccttt tatcttatcg aaaagataaa
       61 ttttttctgg gtccaagtac aatttaattt tttctccggc cttcaaatct tgtaatgatt
      121 tcactcgtgc aataaactga actttttcga cttcaaaata aatgaatgat tcattgccca
      181 tcggttctat tacatcaagc acactctcca gttcaattga tttttctgaa cttttctcaa
      241 ccgatatgtc ttccggtctt ataccaattg ttatttcttc tactgtacta ttttttagat
      301 aatctttttg tcggtctgta agatggaaat gaaattcaga attattactt tcaaacatca
      361 atccgttctt ttccgatatt ttcccactta ggaagttcat cgccggactg ccgatgaatc
      421 ccgctacgaa ttgatttact ggcttgttat ataagtttaa tggtgtatcc acctgattta
      481 ttatcccatc tttcattatc actatcctat cccccatcgt cattgcctct gtctgatcgt
      541 gagttacata gatcattgtt gcaccaagct gtttatgaag cttggatatc tctgttctca
      601 tctgaaccct aagttttgca tcaagattac taagtggttc atcaaacaaa aaaacctttg
      661 gtttacgaac aattgctctg ccgactgcaa ctctttgtct ttgtccacct gatagtgctt
      721 ttggttttct atcaagataa gtttccaatc cgagaattct ggctgcttct tttacacgaa
      781 catctatttc cttcttatca actttcttca actttaatcc aaatgccata ttttcataaa
      841 ctgtcatatg tggataaagt gcatagttct gaaataccat tgctatatca cgatcttttg
      901 gcggcagatt atttacaagt ttaccatcaa taaatatttc tccgccggat atttcttcta
      961 gtcctgctat cattcttaag gtagttgtct ttccgcagcc cgatggaccg acaagtacta
     1021 caaattcttt atcttcaact ttaaaatttg cattcgttac aaccttgttt ccgccatcat
     1081 atattttagt tacatttttt aatagtactt cggccataat aatcctttat ggaatttcat
     1141 tattctgttc atattgtttg taccatttgt acatcgggac tgtaattgga aatgcagcga
     1201 tctttccgcc gaatccaata aatctatagg taaaagtatt aatttcagaa gactcaatct
     1261 tttgtaacaa ctgttcatca agattgattt tgtactttct agctagtgaa acagttttat
     1321 catttataag attatccatt ttagtaagtg cagtctttaa acgaataaat tttaaactat
     1381 cctgatctat aatagatttc tgtttgtcgt ttcgattttt cttttcgatt aatttaaata
     1441 ttgaatagcc atcattgact ttcataggcc catatacctg cccgatattt aaaccttgtg
     1501 caattcttcc tatct
//
LOCUS       DASWBY010000192         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_9046, whole genome shotgun sequence.
ACCESSION   DASWBY010000192 DASWBY010000000
VERSION     DASWBY010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9046"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VF870_03445"
     CDS             complement(<1..693)
                     /locus_tag="VF870_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX9251267.1"
                     /translation="MQIGIVGLPFTGKSTLFQTITKTLLDPNELTKSEAHQAMIKVPD
                     ARLDKLTEMFVPKKKVNATIEVVDVVGLQKGDSGSTQFTGKFLGKVKTNDALVQVVRL
                     FENETVPHPDGSINMMRDINSFETEFILSDLAILEKRLETIKKQILKTQDDNLKRELP
                     VLEKCYEILQTEKPLRDASLTKDELHILKTYQLLSVKPMLIALNFGESQINDTEKYME
                     ELVKHKLGHNTKA"
     gene            1177..>2524
                     /locus_tag="VF870_03450"
     CDS             1177..>2524
                     /locus_tag="VF870_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX9251268.1"
                     /translation="MSASNKILLNESELPENYYNIQADMPNPMLPPLHPGTKQPIGPQ
                     DLAAIFPMELIKQEVSKERYIEIPDEVRKIYKLYRPTPLYRAFELEKLLDTPAKIYYK
                     YEGVSPAGSHKPNTAIAQAYYNKMEGAKGLTTETGAGQWGSALSFACQKFGLSCEIYM
                     VKISYDQKPYRKVMMNTWGATVIASPSRNTNSGRTILAQDPNSPGSLGIAISEAVERA
                     ATTEGINYALGSVLNHVLLHQTVIGQEAIKQLEMAGDFPDIVVAPFGGGSNFAGIAFP
                     FLRYNFQEGKNIRCIAVEPASCPKLTKGEFRYDFGDTVGLTPLIPMYTLGHTFVPEPI
                     HAGGLRYHGAGAIISQLLKDKLIEASAIAQLECFDAGVKFAQTEGIIPAPEATHGIAQ
                     VIREAKKAKVEGKQKTILFNLCGHGYFDMSAYQAYFNGELQDHELTDTELYTALREL"
BASE COUNT          808 a          470 c          448 g          798 t
ORIGIN      
        1 agcttttgtg ttgtgtccga gtttatgctt aacgagttct tccatatact tttcagtatc
       61 atttatctgc gactcgccaa aatttaaagc tattaacatc ggcttgacag aaagcagctg
      121 ataagttttt aaaatgtgta attcatcttt tgtaagactt gcatcgcgca atggtttttc
      181 agtctgcaaa atttcataac atttttctag tacgggcaat tctcgtttga ggttgtcatc
      241 ctgagttttt aaaatttgct ttttaattgt ttccaatctt ttttcaagaa ttgccaaatc
      301 ggataaaata aattcagtct caaatgaatt tatatcacgc atcatattta ttgagccgtc
      361 cggatgagga acagtttcat tctcaaataa tcttacaacc tgaacaagtg catcattagt
      421 ttttacttta cctaaaaatt ttcctgtaaa ctgtgttgag cctgaatcac ccttttgtaa
      481 ccctacaaca tcaacaactt caattgtagc attaactttt ttcttaggta caaacatttc
      541 agtcaattta tctaaccgag cgtcaggcac ttttatcata gcctgatgtg cttctgattt
      601 tgttagttcg tttggatcaa gtaaggtttt tgttattgtt tgaaaaagtg ttgattttcc
      661 tgtaaatggc agtccgacaa ttcctatttg catttattcc tctggttaaa attcttaatg
      721 attatgtaca aatatacgaa gattgacgtg taataagatt taattgttgt aagaaaatga
      781 aagtcattcg cgtgcaaaag cgaatccatc actattacat aaatagattc ccgccttagc
      841 gggaatgacg tactcattct agaaattttc agctttatat tttaacggat ttctctcccg
      901 aaaaatcggg accgatatga ctttcaattc tttctcccta tgaattagca gaacatatct
      961 cttgacattc aaaacatcaa attctatctt gcaacagaaa ttcaaataaa aaaacttgaa
     1021 caaaaaactt acatataact tcttttctac ctcagctacc cagacttggc gttggcgcct
     1081 ctaattattt acatattcta aaaatccgta cgatcttttt aaaacttagt taatcactaa
     1141 ttattcattc aattattaat cataaaggag tgctctatga gtgcatcaaa taaaattctg
     1201 ttaaacgaat cagaactgcc tgaaaactat tataacattc aggcagatat gccaaatcca
     1261 atgctgccgc ctctgcatcc gggcacaaaa caaccaatcg gtccgcaaga tctcgcagca
     1321 atatttccta tggaattaat aaagcaggaa gtttctaagg aaagatatat tgaaattcca
     1381 gatgaagtaa gaaaaatcta taaactttat cgtccaaccc cactttaccg tgcctttgaa
     1441 ttagaaaaac ttttagacac gccggcaaaa atttattaca agtatgaagg tgtaagtccg
     1501 gcaggttctc acaaacctaa tacggcaatt gcgcaggcat attacaataa gatggaaggt
     1561 gcaaaaggtt taacaactga aaccggtgcc ggtcaatggg gcagtgcttt aagttttgca
     1621 tgtcagaaat tcggattaag ctgtgagatt tatatggtaa agatcagtta tgatcaaaaa
     1681 ccttacagaa aagttatgat gaatacatgg ggtgcaaccg tcatcgcttc accgagcaga
     1741 aatacaaatt ctggcagaac aattctagct caggatccta attcaccagg cagcttggga
     1801 attgcaattt ctgaagcagt tgaaagagcc gcaaccaccg aaggcataaa ttatgcactc
     1861 ggcagcgtgc ttaatcatgt tctacttcat caaactgtaa ttggacagga agcaataaaa
     1921 caattagaaa tggcagggga ttttcctgat attgttgttg caccatttgg cggcggatca
     1981 aattttgcag gaattgcttt tccattttta cgttacaatt ttcaggaagg aaaaaatatt
     2041 cgctgtatag ccgttgaacc tgcatcttgt ccaaaactta ctaaaggaga gtttagatat
     2101 gattttggtg atacggttgg attgacgccg cttattccaa tgtacactct tggtcataca
     2161 ttcgtacctg aacctattca tgctggagga ttgcgctatc atggtgctgg agcaattata
     2221 agccagttgt taaaagataa attgattgaa gcatctgcaa tcgcacagct tgagtgtttt
     2281 gatgcaggtg ttaaatttgc tcagaccgaa ggaataattc ctgcaccaga agcaacacac
     2341 ggaattgcac aagttattcg cgaagcaaaa aaagcaaaag ttgaaggaaa acaaaaaact
     2401 attttattta atctttgcgg acatggctac tttgatatga gcgcttatca agcctacttt
     2461 aatggtgagt tacaggatca cgaattgacc gatacagaac tttatactgc tttgcgagaa
     2521 ttag
//
LOCUS       DASWBY010000193         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_44326, whole genome shotgun sequence.
ACCESSION   DASWBY010000193 DASWBY010000000
VERSION     DASWBY010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44326"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VF870_03455"
     CDS             <1..699
                     /locus_tag="VF870_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251269.1"
                     /translation="VRNDLQIWKENYLAQLYFNNNLDSIKLTDKDVYNYYIDELVNSS
                     NIRLLNIRLVSTKDLDEVSNIFDLLKKGKDFGDIVKSFGKTDSLVNEYGETGLKPVLL
                     LGYVGYVASDLNLNEIYGPIKRNNAYTILQVIEKQESSDTLKLSFDSIKDQLRNDLRF
                     KKLNERLKKITSDLAQQNNVKIYADVIDKIQTSQIPMFVHRLMGFGGRIAGVPLTTPF
                     SGWINEEVKQELLP"
     gene            complement(711..>1692)
                     /locus_tag="VF870_03460"
     CDS             complement(711..>1692)
                     /locus_tag="VF870_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX9251270.1"
                     /translation="GLDEYNNYVIANYTTGGIISGAMSWNRVKNLSLLTYLNSLQPGA
                     SQSVKNQIKNSLVSYANTLLSRASANGFGVTINPGEYYWGCNSDVLNKALLLVYAYEQ
                     TNNAAYYQTALMQLNYILGTNAHNLSFLTGAGSNSVMHPHHRPSVADGVAEPVPGLLA
                     GGPDQFLDDPVLQAHFNSSTPPALCYIDDVGSYASNEIAINWNAPLVFVSGYFNVEGV
                     SSVDDQGLNIVPDKFELEQNYPNPFNPITTITWQSPVSGWQTLKIFDILGNEISTLVD
                     EFRTAGRYEVTFSADQSLDGLSSKAITSGIYFYRLEIGDFFTTKKMVLLK"
BASE COUNT          560 a          304 c          303 g          525 t
ORIGIN      
        1 gtccgtaatg atttgcagat atggaaagaa aattacctgg ctcaattata ttttaataat
       61 aaccttgatt caataaagtt aactgataaa gatgtttaca attactacat cgatgagtta
      121 gtaaattcat ccaacatcag attattgaac attagattgg tttctactaa agacctggat
      181 gaagtatcaa acatttttga tttgcttaaa aaaggaaaag attttggcga cattgttaaa
      241 agtttcggca aaaccgattc tttagtaaac gaatatggtg agacaggatt aaaaccagtt
      301 ttgctgctcg gctatgttgg ctatgttgca tcagatttaa atttgaatga gatctacggt
      361 ccaattaaaa ggaataatgc atacacaata ctgcaggtaa ttgagaagca ggaaagcagt
      421 gatactttaa aactatcatt tgattcaatt aaagatcagc taagaaacga tttacgtttt
      481 aaaaaattaa atgaacgtct caaaaaaatt acatccgatc ttgcacagca gaataatgtt
      541 aaaatttatg ctgatgtgat tgataaaatc caaacatcac agatcccaat gtttgtacat
      601 cgtttaatgg gatttggcgg aaggattgcc ggagtaccgt taactactcc cttttccggc
      661 tggataaatg aagaagtaaa acaggagtta cttccttaat tttctaaaat ttatttgaga
      721 aggaccatct ttttagttgt gaaaaaatcc ccaatttcta atctatagaa atatattccg
      781 ctcgtgatgg ctttactgga tagaccatcc aaactttggt ctgctgaaaa tgtaacctca
      841 tacctccctg ctgttctgaa ttcatccacc aaagttgaaa tttcatttcc aagaatatca
      901 aaaattttta gtgtttgcca tccgctgaca ggtgactgcc aggtaatcgt agtaatggga
      961 ttaaatggat taggataatt ttgttcgagt tcaaatttgt ccggtacaat attcaatcct
     1021 tggtcatcaa ctgagctaac accttcaacg ttaaagtaac cggaaacaaa aaccagggga
     1081 gcattccaat ttatagcgat ctcgtttgaa gcataacttc caacatcatc aatataacaa
     1141 agtgccggcg gtgttgaact attaaaatgt gcctgcaaaa caggatcatc caaaaactga
     1201 tccggacctc ctgctaataa ccccggtaca ggttctgcaa ctccatctgc tactgatgga
     1261 cgatgatgcg gatgcataac agagtttgat ccagcaccag ttaaaaatga gagattatgt
     1321 gcattagttc caagtatgta atttaattgc atcaaagctg tctgatagta tgctgcattg
     1381 tttgtctgct cgtaagcata aactagcagt agggctttat tcagaacatc ggaattgcag
     1441 ccccaataat attcaccggg atttattgta acaccgaatc cgtttgcact tgccctgctc
     1501 aataaagtgt ttgcataact taccagactg tttttaatct ggtttttaac agattgtgaa
     1561 gcaccaggct gtaaactatt taaatatgtc agcaacgaaa gatttttaac tctattccag
     1621 gacattgctc ctgaaattat tccaccggtg gtatagtttg ctattacgta attattatat
     1681 tcatctaaac cg
//
LOCUS       DASWBY010000194         5510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_194514, whole genome shotgun sequence.
ACCESSION   DASWBY010000194 DASWBY010000000
VERSION     DASWBY010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194514"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /gene="lepB"
                     /locus_tag="VF870_03465"
     CDS             <1..410
                     /gene="lepB"
                     /locus_tag="VF870_03465"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9251271.1"
                     /translation="NKDLFPYNNKWSLENYGPIVVPKKGMKVELDPKNILIWQNAINT
                     DYGKKVISVEGTVITLNNQPIRDYIFEKDYYFVLGDNRKNSIDSRYFGFIPEEWIIGK
                     AFMIYWSQLPTQTSGISDYVNSIRFSRLMQSIE"
     gene            478..2451
                     /locus_tag="VF870_03470"
     CDS             478..2451
                     /locus_tag="VF870_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9251272.1"
                     /translation="MKYILILLLILSSQIFPQEKYFIYFTDKGGENSKVLNKTSASYI
                     QALQDLSPKAIERRIKNMGEDFINYDDLPITKNYIDEIEKLGIKIIRKLSWFNSVSAV
                     LTSDQINILESLPFIKSIEPVKKLYFQNDPLINSGQQLFKQADSDYGYGVSFNQMNLS
                     DVPLVHSKNINGQNVLIGILDSGFDWKFHNSLKDRNVIAEYDFIFEDSVTANQEGDSP
                     SQDSHGTYVFSIIAGFADSILIGPAFNSSFILAKTEDIRSETHIEEDNYAAALIWMES
                     YGVDITTSSLGYNIFDSGYSYTYSDMDGRTTIVTKAADLAFQRGVSTFTAAGNEGNDA
                     WKYIIAPADAFNIISVGAVDASGNYASFSSQGPTFDGRIKPEVVAQGVNTYGAVAGTM
                     DGYRFANGTSAATPIASGVVALLLSAHPYLKNTQIRSIILETSSNSKNPNNQIGYGII
                     SAKNAIEFPNLENVNDSYVLHKTFLDDNIIASSVNVVFQIADDLFEAFPMIKSGDYDF
                     TYTIPIKNAGELVEFSIIYSDSQNNSYIIPQTGKFKFNYGSDIISLNLDIETPNLNYE
                     VSDFYPNPFIPSKHKNTRINFISTGKEIFKLMLIDGTGQKVKEVSTTTFAGENYFEWD
                     GFSDRGYLCASGVYYALIQLGGKEFGKKLVLLK"
     gene            2493..3134
                     /locus_tag="VF870_03475"
     CDS             2493..3134
                     /locus_tag="VF870_03475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX9251273.1"
                     /translation="MSQILFPAQKKYLESFRKKGDDLILEMERYANEHNVPILDWHSA
                     DFIEKLILINKPDRVLEIGTAIAYTTIRIAKCLHKGSVIHSIEKSDDNIKIALENIEK
                     SGVSKKIKLLQGDALSVMPQLNKKYDFIFLDADKEDYKRLFDYSMILLRKGGVIVVDN
                     LLWHGYAASNRVPTKYGKSTKHIKDFNRLFILQQNLDSIIIPIGDGIGLGIKK"
     gene            3137..3565
                     /locus_tag="VF870_03480"
     CDS             3137..3565
                     /locus_tag="VF870_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251274.1"
                     /translation="MNKVHPNFLNDVEKFYGKSLSKKNDLTIILQAIDTDEKYKDFEN
                     LSFTGKYLNGLFRVLQNSVKIPEVENVDQIKKDLSDNMEKVISQLKEIASSTNELNKK
                     YFEENYFQLSQSAIQNLQQLVEDLDIIKKYLNHLKRNDPK"
     gene            complement(3571..4017)
                     /locus_tag="VF870_03485"
     CDS             complement(3571..4017)
                     /locus_tag="VF870_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9251275.1"
                     /translation="MELNHNAERLANLTFSLLANCQEKEVRLAEVHSLTQAEFRCLRL
                     FGSDESINNKAIAERMNLSPSRLTRIIDGLVQKEYIIREIDPNDRRNMRVSLSRKGQI
                     LVHQLNKAYIEIHKEILQDIEVDQHQPLITAMTHLLSALEKWIAKT"
     gene            complement(4109..4729)
                     /locus_tag="VF870_03490"
     CDS             complement(4109..4729)
                     /locus_tag="VF870_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEX9251276.1"
                     /translation="MLPEQIKTIKDSSQISIKEKGSEFIAIVYPVDSEESTVNQLSVI
                     RKKYFDATHHCYAYRLKSGEEKYSDDGEPKGTAGIRILNAIEHFDLCDVLAIVVRYFG
                     GTKLGVGPLGKAYYNASIDVLEHSEILTKTLHHLIQIETDFNFINHVHRIINHYSAII
                     ADSEYSNIVKFNCYIKPNDFEKINIELTNLTNGQILIKTLETYKYF"
     gene            4838..>5510
                     /locus_tag="VF870_03495"
     CDS             4838..>5510
                     /locus_tag="VF870_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9251277.1"
                     /translation="MEKDTIIIKGAREHNLKNIDVEIPRDSFTVITGLSGSGKSSLAF
                     DTIYAEGQRRYIESLSAYARQFLDMLEKPEVDLIEGLSPAISIEQKSTSGNPRSTVGT
                     VTEIYDYLRLLYARIGVPHCYNCGKPVTKQTSAQIIDTIIQNQSGKKVNVLAPVIRGR
                     KGHYRELFEEIISDGFLKVRVDGEYFEIEKGFHVDRYKIHNIEIAVDKVNASERSRGR
                     LTESVD"
BASE COUNT         1929 a          840 c          939 g         1802 t
ORIGIN      
        1 ttaacaaaga tctttttcca tacaataata aatggtctct ggaaaattat ggtccgattg
       61 tagtccctaa aaaaggaatg aaagttgaac tagatccgaa aaatattctt atctggcaaa
      121 acgctattaa tacagactat gggaagaaag ttattagtgt tgaaggaaca gtaattacac
      181 ttaacaatca accgatacgt gattatattt ttgaaaaaga ttattatttt gttttaggcg
      241 ataatagaaa aaacagtata gatagtcgtt actttggatt cattccggag gagtggataa
      301 taggaaaagc attcatgatt tattggtcac aattacccac tcaaacatca ggcatatcgg
      361 attatgttaa ttcaatcaga ttcagccgat tgatgcagtc aatagaataa aaaataactg
      421 ataaattttt aatcttttta ttgtttcagc aattattagc tttacgaaaa aaatataatg
      481 aaatacatac taatactatt attaatttta tccagccaga tctttcctca ggaaaagtac
      541 tttatatatt ttacagataa aggcggagag aactctaaag ttttaaacaa aacaagtgca
      601 tcatatattc aagctttaca agatctttct ccaaaagcaa ttgagcgtag aataaaaaac
      661 atgggagaag attttataaa ttatgatgat cttccgatca ccaaaaatta tatcgatgaa
      721 atagagaagc ttggaattaa aattatccga aaactaagct ggttcaattc cgtatccgca
      781 gttttaacat ctgatcaaat taatatcctt gaatcacttc cgtttattaa atcaatagag
      841 cctgttaaaa aactttattt tcaaaacgat cctttaataa attccggaca acaattattt
      901 aaacaagcag attcggatta tggttatggt gtttcattta atcaaatgaa tttatctgac
      961 gtccccttag ttcattcgaa gaatattaat ggacagaatg ttctaatagg aatactggat
     1021 tccggatttg attggaagtt tcataattcc ttaaaagaca gaaatgttat tgctgagtac
     1081 gattttattt ttgaagattc cgttactgca aaccaggaag gggatagtcc ttctcaggat
     1141 agtcatggaa cttatgtatt ttcaattatt gccggatttg ctgatagtat tttaatagga
     1201 cctgctttta attcatcatt catactagca aagacagaag atattcgaag tgaaacacac
     1261 attgaagaag ataattatgc ggcagcatta atatggatgg aatcttatgg agttgatatt
     1321 actacaagct cacttggtta taatatattt gactccggtt actcatatac ttactcagat
     1381 atggatggaa gaaccaccat tgttacaaag gctgctgatt tggcatttca gcgaggtgtt
     1441 tcaactttta cagcggctgg taatgaggga aacgatgcgt ggaaatatat tattgcacca
     1501 gccgatgctt tcaatatcat ttctgttgga gccgtcgatg catccggtaa ctatgcaagt
     1561 ttcagtagtc aaggtccaac tttcgatgga agaataaaac ccgaagttgt tgctcagggt
     1621 gttaatactt atggcgctgt tgccggaaca atggatggat acagatttgc taacggaact
     1681 tcagccgcaa caccaatagc aagcggagta gttgcattgc tcttatctgc acatccttat
     1741 ttaaagaata cccagataag aagcatcata cttgaaacat cttctaattc taaaaaccca
     1801 aataatcaaa ttggatatgg aattatatct gcaaaaaatg caattgaatt tccaaatctt
     1861 gaaaatgtaa atgatagtta tgttttgcac aaaacatttt tagatgataa tataattgca
     1921 agctcagtaa atgttgtatt tcaaattgca gatgatctgt ttgaagcatt cccaatgatc
     1981 aaatcaggtg actatgattt tacttatact atcccaataa aaaatgccgg tgaactagtc
     2041 gagttttcta tcatttacag cgacagccag aataacagct atataattcc tcaaaccgga
     2101 aaatttaaat tcaattatgg ttctgatata atttcattaa atctggatat tgaaaccccc
     2161 aatctgaatt atgaagtaag cgatttttat ccaaatccat ttattccatc taaacataaa
     2221 aatactcgta taaattttat ttctacaggg aaagagatat ttaaattaat gttgattgat
     2281 ggaacggggc aaaaagttaa agaggtatca acaacaactt ttgcaggtga aaactatttt
     2341 gaatgggatg gtttttcaga tcgtggttac ctatgtgcaa gcggagttta ttatgcttta
     2401 attcagctcg gaggaaaaga gtttggtaag aaattagttc tgctaaaata aaatatatcg
     2461 attacagatt tataatctaa agagtaaaaa caatgtcaca aatattattt cccgcacaaa
     2521 aaaaatattt agaatctttt agaaaaaagg gagacgattt aattcttgag atggaacgtt
     2581 acgcaaatga acataatgtt ccgattctgg attggcactc cgcagatttt attgaaaaat
     2641 taatactaat caacaaaccg gatagagttc tggaaatagg aacggcaatt gcttatacca
     2701 caattagaat tgcaaaatgt ctgcataaag gttccgtcat tcattcaatt gagaaaagtg
     2761 atgataatat aaaaattgct ttagagaata ttgaaaaatc gggtgtaagc aaaaagataa
     2821 agttactgca aggtgatgcg cttagtgtaa tgcctcagct aaataaaaaa tatgatttta
     2881 tttttctgga tgcagataaa gaagattata aaagattgtt tgactattca atgatattat
     2941 tgcgtaaagg cggggttatt gtcgttgata atcttttatg gcacggatat gcagcttcaa
     3001 atcgtgttcc taccaagtat ggcaaatcaa caaagcatat aaaagacttt aacaggttat
     3061 ttatacttca gcaaaacctt gattcaatta taattcctat tggagatgga ataggtttag
     3121 gtataaaaaa ataattatga ataaagtgca tccaaatttt cttaatgatg ttgaaaaatt
     3181 ttacggtaaa tcgttaagta aaaaaaatga tcttacaatt attcttcaag ctatagatac
     3241 tgatgaaaag tataaggatt ttgaaaactt atcttttacg ggaaagtatt taaatggctt
     3301 atttagagtt ttacagaaca gtgttaaaat tcctgaagta gaaaatgttg atcaaataaa
     3361 aaaagatttg tctgataata tggagaaagt aatttctcag ctaaaagaaa ttgcttccag
     3421 cacgaatgaa ctgaataaaa aatattttga agaaaattat tttcagttat cgcaatctgc
     3481 aatacaaaat cttcaacaac tggttgagga tttagatata ataaaaaaat atttgaatca
     3541 cctgaaaaga aatgatccta aatagttccc ttaagtttta gctatccatt tttctaaagc
     3601 tgacaataaa tgcgtcatcg cagtaattaa cggttgatgc tgatcaactt caatatcctg
     3661 aagaatttct ttatgaattt cgatgtaagc tttatttaac tgatgaacaa gaatctgacc
     3721 ttttctagat aaagagactc tcatatttct tctgtcattt gggtcaattt ccctgatgat
     3781 gtattctttt tgaacaagtc cgtcaatgat gcgggttaat ctgcttggac tcagattcat
     3841 tctttctgca atagctttat tgttgatgct ttcatcagat ccaaataatc ttaagcatct
     3901 gaattcagct tgggttaaac tatgcacttc tgctaaacgg acttcttttt cctgacagtt
     3961 tgctaacaaa ctaaaagtta agtttgctaa tctttcagcg ttgtgattta attccataaa
     4021 agtacccatt ttttaataga tgaattattg tctttaacaa atatcgataa taaaaataat
     4081 tatgtcaagc gcagtttgta gatttttctt aaaaatattt gtatgtctcc aaggtcttaa
     4141 tcaagatttg tccattggta agatttgtta attcaatatt tattttttca aaatcattgg
     4201 gctttatata acaattaaac ttcacaatat ttgaatattc agaatcagca atgattgcag
     4261 aataatgatt aatgatacga tgcacatgat ttataaaatt aaaatcagtc tcaatctgaa
     4321 ttaaatgatg aagtgttttg gtaagaattt ctgaatgctc taaaacgtca atagatgcgt
     4381 tataataagc tttccccagt ggtcccactc caagcttggt tccgccaaaa tatcgcacca
     4441 caatggctaa aacatcgcat aaatcgaaat gctcaatagc atttagaatg cggattccgg
     4501 ccgttccttt tggttctcca tcatcagaat atttttcttc accgcttttt aatctataag
     4561 catagcaatg atgagtagca tcaaaatatt tttttctgat tacgctaagc tgatttactg
     4621 tgctttcctc actatcaaca ggataaacta ttgctataaa ttcggaccct ttttctttga
     4681 tcgaaatctg gctagaatct tttattgttt ttatttgttc cggaagcata tgtgtgcgat
     4741 tgaataaacc atgctaaaat tatttgttta tgaatatcac tattcctaaa ataagtgttt
     4801 gaaaatttaa ttctaatttt tttaatttga tcgagcattg gaaaaagaca caataattat
     4861 aaaaggtgca cgagagcaca atctaaaaaa cattgatgtt gagatcccgc gagattcctt
     4921 cacagtaatt accggtttat caggatcagg aaaatcttct cttgcgtttg atacgattta
     4981 cgcagaaggg cagcgcagat acatcgagtc actttctgct tacgcgcgcc agtttttaga
     5041 tatgcttgaa aagccggaag tagatttgat tgaaggatta agtcccgcaa tttcgattga
     5101 gcaaaaatct acttcaggaa acccgcgctc tactgttggc actgttacag aaatttacga
     5161 ttacttaaga ttgttgtatg cacgaattgg agttccgcat tgctataatt gcggcaaacc
     5221 tgttacaaaa caaacttcag cccagattat tgatacgata attcaaaacc aatctggtaa
     5281 aaaagtaaat gtacttgcac cggttatccg cggaagaaaa ggacactatc gtgaactttt
     5341 tgaagaaatc atttctgatg gatttttaaa agttagagtg gatggtgaat attttgaaat
     5401 tgaaaaagga tttcatgttg accgatacaa aatccacaac attgaaattg ctgttgataa
     5461 agttaatgca agtgaaagat cgcgcggaag attgactgaa tctgttgatg
//
LOCUS       DASWBY010000195         6597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_308354, whole genome shotgun sequence.
ACCESSION   DASWBY010000195 DASWBY010000000
VERSION     DASWBY010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308354"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..90
                     /locus_tag="VF870_03500"
     CDS             10..90
                     /locus_tag="VF870_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251278.1"
                     /translation="MVMLLTNQPSIRDVVFFPQMKPEVKS"
     gene            174..1787
                     /locus_tag="VF870_03505"
     CDS             174..1787
                     /locus_tag="VF870_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9251279.1"
                     /translation="MSFKSVKIPYLLFTAIIGIALGVFLDKVFSEDNLRDSIRKFNDV
                     LTFTEKYYVEEVDTQKLVESAINGMLNELDPHSVYIPPEQFTQVEESFRGDFEGIGIE
                     FQIVNDTLTVVSAISGGPSEALGIMSGDRIVKIDDEDVIGITNEDVRKKLRGRAGTEV
                     KVSISRVGVNKTIDYTIVRDKIPLYSVDTHFMINENTGYVSVSRFSETTYDELVDALN
                     DLNKNGMKQLVLDLRGNPGGYLNQAVKISDLFISGKKKIVYTEGRRKEFDEVYYASEP
                     SRFEEIPLIILINRGSASASEIVSGAVQDWDRGLIVGETSFGKGLVQRQFDLPDNSAL
                     RLTISQYFTPSGRLIQRDYKNKKNKEDYYSENASDEEKEGDNINHTAEQDSARPEYKT
                     GKGRIVYGGCGITPDYIVKSGLMTAYTQNLLRKNIFYTFILSYLDKNTETIKQKYGDD
                     LSKFKSDFKITDAMMKSFLDFASSKEVEFVDADFQKDKDYISARLKAQIARNFWKNEG
                     WYSVLLGSDDQLNKALTLFNEANDLAKLK"
     gene            1784..2596
                     /locus_tag="VF870_03510"
     CDS             1784..2596
                     /locus_tag="VF870_03510"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX9251280.1"
                     /translation="MKKFAEAIFLFSILFFCFGFAGSNNLSSSQSGKKIIEVGEEITY
                     VVKYLSFEIGEIKLIVQKEEVDGKDTIYSAIAYINSYEGIPFVNLHQIYETKFDQNQI
                     SHYFKGSILAEDTTFTEYFFNKRKNTLRVLKGNESKNEIWTDSTIAFNRDCQDGLSLF
                     YFARMRTGKKNKVNVPVLINEKLEKTEINFYDEVEDMDIDAVDYDISCVRLDGETEFR
                     GIFGLTGYFEGWFSNDKYAVPIVAKMQVIIGSVTLELRHWKKKGWMPPKYQD"
     gene            2569..3147
                     /locus_tag="VF870_03515"
     CDS             2569..3147
                     /locus_tag="VF870_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX9251281.1"
                     /translation="MDASEISRLNYQDGERKLFPYLDLFPKVHPTVFLASGVKIVGDV
                     KIGEYTSVWYNTVIRGDVHYVKIGKMTNIQDCSMLHVTNGRYPLNIGNKVTVGHSVKL
                     HGCTLQDLCFIGIGAIILDGAVVETNSMVAAGAVVKPDFIVPSGKLVAGVPARIIRDL
                     TTEEIDDFEKSASRYKKYAEITVDSLNKINIK"
     gene            3157..3582
                     /gene="ybeY"
                     /locus_tag="VF870_03520"
     CDS             3157..3582
                     /gene="ybeY"
                     /locus_tag="VF870_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX9251282.1"
                     /translation="MKNLRIYSIARNIKKKNIHLLVKSLSDELNFSISSLEINFVSAD
                     SILSINKKYLKHHYTTDIITFNYSDSGKDIDGEIFISVDDAQINANKFKVSLSDELIR
                     LVIHGILHLLGYDDQTDADKKIMKRLENKLLSKHNFILL"
     gene            3709..4590
                     /locus_tag="VF870_03525"
     CDS             3709..4590
                     /locus_tag="VF870_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9251283.1"
                     /translation="MNINDLIVKYLAEIKNIRRYSDNTIKSYLTDLEEFLRFCDEKQK
                     TKLDDVTERFIKFYLMFLSEKDLDKKTIARKLAALRSTFKYAFQNDLISENPLSFISN
                     PKSSRKLPEVVSTDLILKIYKLADESDENPELIKVVFELLYGCALRVSELCDLKYSSL
                     EMKKAQVRILGKGNKMRIVPIGEKSLIILNEYLSKNPVNSYDDYLIRTQKNDKLYPRL
                     VHRMINKYLNKVSDIKKKSPHILRHSAATHMLDNGADLRAVKEILGHENLSTTQIYTH
                     VSIERLKSTYKKSHPKS"
     gene            4607..4909
                     /gene="raiA"
                     /locus_tag="VF870_03530"
     CDS             4607..4909
                     /gene="raiA"
                     /locus_tag="VF870_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX9251284.1"
                     /translation="MNIQITARKFKAHDTLKEYIKDELSSLKKFNDDIISADVILSYQ
                     NVANSIKKVEVVLNIPGQTLNAADQTEDYKTAVSSVTEKLRRQLETIKSKRVSKTR"
     gene            4906..5919
                     /gene="hprK"
                     /locus_tag="VF870_03535"
     CDS             4906..5919
                     /gene="hprK"
                     /locus_tag="VF870_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX9251285.1"
                     /translation="MIKIDQNTITRKESINVGFLYKNAKNICKLHLLSENNDFDKKIV
                     DQNLHRPGLALAGFVDLFSYTRVQVFGNTEMRYLEQLSEEKLSESLDRIFEFEIPCII
                     LTDDNKPFPLLLKKSNENKVPLFNTSFSTTKLVYLLSDFLDDQFAPRITLHGSFVDVY
                     GVGILFGGKSGIGKSEVALDLIERGHRLVADDVIILTKKGEGILMGSGSELGQKFMEI
                     RGLGLIDIQSMFGVRAIRFQKRLEIVVELESWDENTEYTRTGLDNKFVSIMDVDIPYI
                     KLPIIPGKNITVISEVISLNYLLKHYGHDAAKIFQDRLNERIKNKTNLESRGVDYFEH
                     DFE"
     gene            5980..>6597
                     /locus_tag="VF870_03540"
     CDS             5980..>6597
                     /locus_tag="VF870_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251286.1"
                     /translation="MKKFYYFSEKSLNFLEIKHFKEKAIAVFIVSVLVFSSILFGIFY
                     LISNLSNKDDYLRSLKKENELLKNKFSTLTERYASLESELKSLTDISNELRLAVNLTP
                     LTVEERNLGVGGTNTISKLYNELGSNVSGAIDVADLVTKKFEFEKAQYEEITSKLNEN
                     RSLFESIPAIIPTEGHYSIESFGMRIHPILKLKKMHTGIDIINNVG"
BASE COUNT         2385 a          915 c         1212 g         2085 t
ORIGIN      
        1 attgatagaa tcgtaatgtt attaactaat caaccatcaa ttcgtgatgt tgttttcttt
       61 ccacagatga aacctgaagt taaaagttga tcacggaatt atttattgac tcacaagagt
      121 cattgaaatt gtttatttac tggtaaatta ttttaagtta ttggagcaaa cgaatgtcat
      181 ttaaatctgt aaaaattcca tatttattat tcacagcaat aattggaatt gcgctaggtg
      241 tattcttgga taaagttttt tctgaagata acctccgcga tagcatcagg aaatttaatg
      301 atgtattaac atttacagaa aaatattatg tggaagaagt tgatactcaa aagctagttg
      361 aatcagctat taatggcatg ttgaatgaac tagatcctca ctctgtatat attccgccgg
      421 aacaatttac ccaggttgaa gaatcattcc gtggagattt tgaaggaata ggaattgaat
      481 ttcagattgt taacgataca ttaaccgttg tttcagcaat atcgggcgga ccgagtgaag
      541 cattaggaat tatgtccgga gatagaattg ttaaaataga tgatgaagat gttattggta
      601 tcacgaatga agatgttcgt aaaaaactga gaggaagagc aggaacagaa gttaaagtat
      661 ctataagcag agtcggtgtg aataaaacaa ttgattatac aattgttcgg gataaaattc
      721 cgttatactc agtagacacg cattttatga taaatgaaaa caccggctat gtcagcgtat
      781 caagattttc ggaaacaact tatgatgaac ttgttgatgc attaaacgac ttaaataaaa
      841 atggtatgaa gcagctcgtt cttgatctta gaggcaatcc aggcggttat ttaaatcagg
      901 cagttaaaat aagtgatctt tttataagtg gtaaaaagaa aatagtttat actgagggac
      961 gaagaaaaga atttgacgaa gtttattatg catctgagcc ttccaggttt gaagaaattc
     1021 ctttaattat tcttataaac cgcggcagtg cttctgcaag tgaaattgtt tccggtgccg
     1081 tgcaggattg ggatcggggt ttgatagtcg gtgaaacttc ttttggtaaa ggacttgtac
     1141 agagacagtt tgatttacct gataactctg ctcttcgatt aaccatctcg caatacttta
     1201 ctccaagcgg aagacttatt caacgggatt ataaaaataa aaagaacaaa gaggattatt
     1261 attctgaaaa tgccagtgac gaagaaaagg aaggcgataa cataaaccac acggctgagc
     1321 aagattctgc cagacctgaa tataaaacag gaaagggaag aattgtttat ggcggctgtg
     1381 gaataactcc tgattatatt gttaaatccg gtttgatgac cgcatataca caaaacttat
     1441 taagaaaaaa tattttctac acttttatcc tttcttatct tgataaaaat actgagacca
     1501 taaaacagaa atacggggat gatctatcta aatttaaaag tgatttcaag attacagatg
     1561 caatgatgaa aagttttctt gattttgctt cttccaaaga agtagaattt gttgatgcag
     1621 atttccaaaa agataaagat tatatttcag caagattaaa agcacaaatt gccagaaatt
     1681 tctggaagaa tgaaggctgg tattcagttt tattaggttc tgatgatcaa cttaataaag
     1741 cattaacatt gtttaatgaa gcaaatgatt tagcaaagct aaaatgaaaa agtttgcgga
     1801 agctattttt ctgttttcaa ttttattttt ttgttttgga tttgctggat caaacaactt
     1861 aagttcatca caatccggca aaaaaataat tgaagttggg gaagaaatca cttatgtagt
     1921 taaatatctt tcatttgaaa ttggtgaaat aaaacttatt gttcagaaag aagaagtaga
     1981 cgggaaagat actatttata gtgctatcgc ttacatcaat tcttatgaag ggattccatt
     2041 cgtaaacctc catcaaattt atgagactaa atttgatcaa aaccaaatat ctcattactt
     2101 caaaggctca attcttgccg aagatacaac tttcactgaa tattttttca ataaaagaaa
     2161 aaatactctc agggttctca aaggtaacga aagcaaaaat gaaatttgga ctgattccac
     2221 tatcgcgttt aatcgtgatt gtcaagatgg actttcactg ttttactttg ccaggatgag
     2281 aaccggaaaa aaaaataaag taaatgttcc tgttttaata aatgagaaac ttgaaaagac
     2341 agaaattaat ttttatgatg aagtcgaaga tatggatatt gatgcggttg attatgatat
     2401 atcgtgtgta agattagatg gtgaaacaga attcagaggt attttcggat taacagggta
     2461 ttttgaagga tggttttcaa atgataaata tgctgtgcct atcgttgcaa aaatgcaggt
     2521 aattattggc agcgtaactt tagaattaag acattggaag aaaaaaggat ggatgcctcc
     2581 gaaatatcaa gactaaatta tcaggatggt gaaagaaaac tttttcctta cctggattta
     2641 tttcccaaag tgcatccaac tgtgtttctt gcttcgggag taaaaattgt tggagatgta
     2701 aagattggtg aatacacaag tgtttggtac aacactgtta ttcgtggtga tgttcattat
     2761 gttaagattg gtaagatgac taacattcag gattgctcga tgcttcatgt aactaatgga
     2821 agatatccgt taaacattgg aaacaaagta acagtaggac attcagtcaa attgcatggc
     2881 tgcacactgc aggatttatg ttttattgga attggggcta ttattttaga tggagctgta
     2941 gttgagacca attctatggt tgctgcaggc gctgttgtta aacctgattt tattgttcca
     3001 tccggtaaat tagtggctgg tgttcctgcc agaattattc gtgatttaac tactgaagag
     3061 attgatgact ttgagaaatc tgcctcacgc tataaaaaat atgctgagat tactgttgat
     3121 tctctaaaca aaataaatat taagtaagag tatattttga aaaatttgag aatttattca
     3181 attgccagaa acattaagaa aaaaaatatt cacttattag ttaagtcttt gtctgatgaa
     3241 ttaaacttta gtatttcaag tcttgagata aactttgttt ctgctgattc tattttaagt
     3301 atcaataaga aatatttaaa acaccattat actacagata taatcacatt taattattcc
     3361 gattcaggaa aagatattga tggcgaaata tttatctcgg ttgatgatgc acaaattaat
     3421 gcaaataagt ttaaagtttc attatcggat gaactaatta ggttagtaat acacggcatt
     3481 cttcatcttc taggttacga tgaccaaacg gatgcggata aaaaaataat gaagcgatta
     3541 gaaaataaat tgctttctaa gcataatttt attttattgt agttagaaaa aaacaatata
     3601 agcaagaatc cctcttgatc ccgaaaaaga aaaataaaat agcggatcag tcctcagaaa
     3661 aatattttgg tatagataat atcatacatt ttacattaga tgattataat gaacatcaat
     3721 gatttaattg taaaatattt agctgagatt aagaatataa ggcgctattc agataatacc
     3781 ataaaatctt atctgactga tttagaagag tttcttagat tttgtgatga aaagcaaaaa
     3841 actaaattgg atgatgttac agaaagattt attaaatttt atttaatgtt tcttagtgaa
     3901 aaagatctgg acaaaaaaac aattgccaga aaacttgctg cattaagaag cacattcaaa
     3961 tatgcctttc aaaatgattt aatatctgaa aatccattat catttatttc taaccctaaa
     4021 tcatcaagaa aacttccgga agttgtttca acagatctta tacttaaaat ctacaaatta
     4081 gcagatgaat ctgatgaaaa tccggaacta attaaagtgg tttttgaatt actttacggt
     4141 tgcgctcttc gagtaagcga actgtgcgat ttaaagtatt caagtttaga gatgaaaaag
     4201 gctcaggtac ggatactggg gaaaggtaat aaaatgagaa ttgtaccgat cggtgaaaaa
     4261 tctcttataa ttctaaatga atatttatca aaaaatcctg ttaattctta tgatgactac
     4321 ttgattcgta cacaaaaaaa tgataaactt tacccaagat tagttcatag aatgataaat
     4381 aaatacttga acaaagtttc cgatataaaa aagaaaagcc cgcatattct tcggcatagt
     4441 gcagcaactc atatgttaga taatggcgcg gatttaaggg cggtaaagga gatcctgggg
     4501 cacgaaaatt tatcaacaac tcaaatctat actcacgtta gtatagaacg gcttaaatcc
     4561 acttataaaa aatcacatcc aaaatcttaa aacaacggag gataatatga atatccagat
     4621 aactgcacgc aaatttaaag ctcacgatac tcttaaagag tatataaagg atgaactatc
     4681 ctcattaaaa aaatttaacg atgatataat tagtgctgat gtaattctca gctatcaaaa
     4741 tgttgctaac agtattaaga aagttgaagt tgtgttaaat ataccaggac aaacacttaa
     4801 tgcagctgat cagacagaag actataaaac agctgtaagt tcagttacag aaaagttgag
     4861 gcgacaatta gaaaccatta aatcaaaaag agtctcaaaa accagatgat taagattgat
     4921 cagaatacaa taacccgtaa agaaagtatt aatgtcggtt ttctttacaa gaatgcaaaa
     4981 aatatatgca aattacattt gttaagtgaa aacaatgatt ttgataaaaa aattgtagat
     5041 cagaatcttc atcggccagg gttagctctg gccgggtttg ttgatttatt ttcctatact
     5101 cgtgttcagg tattcggtaa tactgaaatg agatatcttg aacaacttag tgaggaaaag
     5161 ctgagtgaat cacttgatag gatttttgaa tttgaaattc cgtgtattat tcttaccgat
     5221 gataataaac catttccgct tttattaaaa aagtcaaatg aaaataaagt tccattattt
     5281 aacacaagtt tttcaaccac aaaattggtt tacttattaa gtgattttct tgatgaccaa
     5341 tttgcaccca gaataacttt acatggatca tttgttgacg tttatggtgt tgggattcta
     5401 tttggcggaa aatcaggtat cggaaaaagt gaagttgcac ttgatttaat tgagcgtggg
     5461 catagactgg ttgcagatga tgtaattatt ctcaccaaaa aaggtgaggg tattttaatg
     5521 ggatcaggat cagaattagg acagaagttt atggaaattc gtggtcttgg tctgatcgac
     5581 attcaaagca tgtttggtgt aagggctatt agatttcaaa agagacttga aatagttgtg
     5641 gaacttgaat cctgggatga aaatacagaa tacacaagaa caggattgga taataaattt
     5701 gtttccataa tggatgttga tataccgtat attaaattac caattatacc tggaaagaat
     5761 attactgtta tctctgaggt tatcagccta aattatttgc ttaagcatta tggtcatgat
     5821 gctgctaaaa tattccagga taggcttaat gagagaatta aaaataaaac taatctggaa
     5881 agcaggggag tcgactattt tgagcacgat tttgagtgat ttttaatgaa tttagatgtt
     5941 gacaaaacaa agcaaaatct ttaagttgcc gcacaattta tgaaaaagtt ttactacttc
     6001 tcagaaaaga gccttaactt ccttgaaata aagcacttca aggaaaaagc tatagcagtt
     6061 tttattgttt ctgtgttagt atttagctcc attttattcg gcattttcta cctcatatca
     6121 aatctctcca ataaggatga ttatttacgc tcattgaaga aggaaaatga acttttaaag
     6181 aataaattct caacactgac cgaaagatat gcaagtctcg aatctgaatt aaaatcatta
     6241 acggatatca gcaacgaatt aagattagct gttaacctta ctcctttaac cgttgaggaa
     6301 agaaatttag gagtcggtgg tacaaacaca atctcaaaac tttacaacga attaggttca
     6361 aatgtttctg gtgcaattga tgttgcagac ttagttacga aaaaatttga atttgagaaa
     6421 gcgcaatacg aagagatcac atctaagctt aatgaaaata gaagtttatt tgaatctatt
     6481 cctgctatta ttcctacaga aggccattac agtatcgaaa gttttggtat gcgaattcac
     6541 ccgatcctga agcttaaaaa aatgcatact ggaatcgata ttattaataa tgtggga
//
LOCUS       DASWBY010000196         6514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_345021, whole genome shotgun sequence.
ACCESSION   DASWBY010000196 DASWBY010000000
VERSION     DASWBY010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345021"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VF870_03545"
     CDS             complement(<1..1116)
                     /locus_tag="VF870_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX9251287.1"
                     /translation="MNRFRNWFLVLLIIFISFQLVAQKKNIIGCYQSWKFQQYPELLS
                     PQNIPYDKLTIINYSFFYPLESGEIVGMDPAADLYLLKGETDSLSANIAREGSIIELA
                     KHHGTKVVLSIGGWETSNNFPQVSADPKKRANFAHWCIKHIQQYDFDGIDIDWEFPGY
                     KKHNGTEQDRENFTLLLQTIRDSLQAFEDKSGKSLLLTVSLPAAAVHLLDIEVEKISS
                     IVDFMNIMTYDLYGSWGKISNHNSALYAPAQGDSARCLDGAFKLYHETHNVPAEKITL
                     CAAFFGYSYTNCREIYGEHQGADTILFKAGEDILYSQIAEKMDMFERYWDDKAQAPYL
                     ISKSNSTLISFDDEESVALKSEYIIENNAGGLIIWPLM"
     gene            complement(1266..3434)
                     /locus_tag="VF870_03550"
     CDS             complement(1266..3434)
                     /locus_tag="VF870_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251288.1"
                     /translation="MATIKPGLHRFLIIQITFILFANYSSAQQPWKPNTDWGYWVLGQ
                     KPDLGFLEKNNMTVTFGSGAPNFDEVTRSEFDTKMVEAKKFNQSYHDKGYIVLRYLST
                     SLNGNSNSNKDIPTKEQLNYLKFYNERWQDFEDYIGPKPPEDPTTWMMIRPDGTFPYY
                     RYAPYGKETDEGFEAWGVPVNPYYVRLMEGKIRAQAETGIDGSYIDWTHIAEETSYDN
                     YSRLGFIDYLKNNLPAEVSNKKYGMSDFTEIKLPERRGDKFWMEWITYRGYQVAEFHK
                     HMRDVARQYNPNFMISGNVFGGFGYGPIAYLGAGNMEMLARDGYDDFIYSEMQEYLDA
                     APRNKDGVKITNGPALKFLTAVAHGKPVIVYATEITPPIFPNPTEKCLSAMAQINIAE
                     AIANHCIFREKRETPQGATDLYKFLAEYREYLIGAQLFSNVGILASLNQYLADEQSFA
                     FTTSRVLSDVGINHTFLVEADLASSGLNQFEVIILPYLPLMNEKNQDALINFVKEGGN
                     LFILGQTGVKNQYNLSNDHIPFLEVNALSEFPKDKLVKSLGNGNMIFIPLQIPENKFL
                     TTMEIKGDVTTFGSSMVNIFADVPEAYTRNKIHPELRQILENLANEMTNMLSDHLTIL
                     KNGSPFVELTTMRKNDKYILVHLVNYNVTVDGDITPATDVNAQISIPEGLSVKAVTYS
                     GELGEMKNLKFDSDESDGKNIVNLTFPSLNIYGLAKIELE"
     gene            complement(3446..5788)
                     /locus_tag="VF870_03555"
     CDS             complement(3446..5788)
                     /locus_tag="VF870_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251289.1"
                     /translation="MNKFISLLFFSLIIGNSAFCQFNFENPRVAIIISKSSFTHHWGV
                     TQMSAHGWGGVANLAGVPYDCISAEDLSEDMNLKKYDCLIFTQCTYLSDAVYESTVKA
                     LENYLSEGGNLIIDGLIGYFDEKTKERDHSKLNQLLDIKYIGFYGDPDYRIKVKDNDQ
                     FITKNYLKGDFVTQHIAGGLNVQTFENDGRILLELTNEKETYPFLSVRQTSKNRIVLI
                     NDFATWSSVPSFFRNVQPQVFYKNQIYNLLIESVYWSLYDSFEDPIPSLQVSNADLTA
                     IIRLDADASGNLNAQIQTINYLIDIANETGVVPVYAWVSSTAATAGWQDIAPLGKKLE
                     NVGGQIGTHSKFHNIDQNMNEQRWKEELDDAIKEIEFNMMDYGYGIGKVDCFINPGNT
                     IHMSDYNQIAKRFGFYMTHGFEQDMPIGFGNFTWFNEDQKNFVVLENTPSPDYQWFYD
                     PTWSYTTQQITAYEESIFDHLYDNIKRGVIFNQMWHDYSITSQPQYGQDRIINTNNIA
                     MYDAIKTKFKNNNIYCPEPEDLRYKICIMAQSNYSWSADGNQLNFKFDISNLGLDSAN
                     LYTGGMGIRINNSSKKIDKVFINNIEHFAFNDNLVILPNISGSIADLKIILADESNVE
                     PHLTYISKPVTKLEKNEDGLILNVLTKSKAKFSFNAPEGYLLLNADGFNYSGINPNEI
                     CGYTNTDRSVRLKKIKSNKFSLLSSTINISDISETENEITLKLKSTAADSYQMNFNTP
                     QGISRIFFDNTEVALQSIGNKYSIVLGPFDGEKDLTIKLK"
     gene            complement(5906..>6514)
                     /locus_tag="VF870_03560"
     CDS             complement(5906..>6514)
                     /locus_tag="VF870_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251290.1"
                     /translation="DAVIQICPCGTSYSFFNLHYMNQTVASDPASSWQIRLKGKTIKA
                     LMGINAPYFGDHVELSDSASDFASTVGIGGIVGTKFTYPTDRLKHKNYLLTPEKEIIW
                     QKWLDIYKENYLSKADYRGELYDIGYDKPETHAVQKGDTMYYAFYADKWNGEVELRGL
                     ENKAYKVIDYVNDKFISNVDGKNPKLNVSFNKYLLIKAVINN"
BASE COUNT         1967 a         1270 c         1078 g         2199 t
ORIGIN      
        1 catcagaggc caaataatta aaccgccggc attattttca attatatatt ctgatttgag
       61 agcaacggat tcttcatcat caaacgaaat aagtgtacta tttgatttgc tgattagata
      121 tggagcctgt gccttatcat cccagtaacg ttcgaacata tccatctttt cggctatttg
      181 cgaatagaga atgtcttctc cagctttaaa taggattgta tcagcccctt gatgctcgcc
      241 ataaatttca cggcaattag tatatgaata tccaaaaaaa gcagcacaca gggttatttt
      301 ttcggccgga acattatgcg tttcatgata taatttaaat gcaccatcca ggcagcgggc
      361 tgaatcacct tgagccggtg cgtacaatgc tgaattatga ttgctgatct taccccagga
      421 tccatataaa tcgtaggtca taatattcat gaagtccact atagaagaga ttttttctac
      481 ttcaatatcc agcagatgaa ctgcagcagc cggtaaggaa acggttaaaa gtaaactttt
      541 acctgattta tcttcgaatg cttgaaggct atcacgtatc gtttggagca gtaaggtgaa
      601 attttcacga tcttgttcgg taccattatg ctttttatat cccgggaatt cccagtcaat
      661 atcgattcca tcaaagtcgt actgctgaat gtgtttgata caccagtgtg caaaattagc
      721 acgtttttta ggatcggcag aaacttgcgg aaaattatta gatgtttccc atccaccaat
      781 tgataaaacg actttagttc cgtggtgctt ggccaactcg attatagatc cttcacgggc
      841 tatatttgct gataacgaat ctgtttctcc tttaagtaaa tagagatcag cagcaggatc
      901 catcccaaca atttcaccac tttccaaagg ataaaaaaat gaatagttta ttatagttaa
      961 tttatcataa ggtatatttt gcggactgag aagttccgga tattgttgga atttccagga
     1021 ctgatagcac ccgattatat tttttttttg agcaactagc tgaaacgata taaaaataat
     1081 aagcaaaaca agaaaccaat ttcgaaatct attcatttta atttacctat ctgttttttt
     1141 aaatcagaga ttaatttcta gaattcttga tttaaaattc ttttgatatc aaccttatga
     1201 ttatttccaa actaatcgtt acctaaacta aaacagaggc ccggtaacat tattgtaatt
     1261 tcgtactact ctaattctat ttttgccagt ccataaatgt tcaaactcgg aaaggtaaga
     1321 ttcactatat tttttccatc actttcgtcg ctatcaaatt ttaagttttt catttctcct
     1381 aactcaccac tataagtaac agcctttact gataggcctt caggaatgct aatttgcgca
     1441 ttcacatctg ttgccggagt aatatcccca tcaactgtta cattataatt gaccaggtga
     1501 accaggatat atttatcatt tttccgcatt gtggttaatt cgacaaatgg tgatccattc
     1561 tttagaatag taagatgatc ggagagcata tttgtcattt catttgcaag gttttccaga
     1621 atttgtctga gttcaggatg tattttgtta cgcgtataag cttccggaac atcggcaaaa
     1681 atatttacca ttgaagatcc aaatgttgtt acatcgcctt tgatttccat tgttgttaaa
     1741 aacttatttt ccggaatttg taatggaata aaaatcatat tcccattgcc tagagactta
     1801 acaagtttat ctttaggaaa ctcagacaat gcattcactt caagaaacgg aatatgatca
     1861 tttgaaagat tgtactgatt cttgacacct gtttgaccaa gaatgaaaag attgccgcct
     1921 tcttttacaa aattaattaa tgcatcctga ttcttctcat tcattaacgg taagtatgga
     1981 agaattatta cttcaaattg attcaacccg gatgaagcaa gatcagcctc aactaaaaat
     2041 gtgtgattta ttcccacatc agaaagaacc cgtgaagtgg tgaaagcaaa actctgctca
     2101 tccgccagat actgatttaa tgatgccaaa ataccaacat tactaaacag ctgtgctccg
     2161 atcagatact cccggtactc tgctaaaaat ttgtaaagat cggtagcacc ttgaggggtt
     2221 tctcttttct cacggaatat gcaatgattt gctatggcct ctgcaatatt aatctgggcc
     2281 attgcactca aacatttttc tgtcggatta ggaaatatcg gaggagtaat ttctgtagca
     2341 taaactatta caggttttcc gtgcgcaaca gctgttagaa actttaaggc cgggccgttc
     2401 gtaattttca ctccatcttt atttctcggt gccgcatcaa gatactcctg catttcagaa
     2461 taaataaaat catcataccc atcccgagct aacatttcca tattacccgc accaaggtat
     2521 gcaattggtc cataaccaaa tccaccgaac acattccctg aaatcataaa gttggggtta
     2581 tactgccggg caacatctct catatgttta tgaaattctg caacctgata accacgatat
     2641 gtaatccact ccatccaaaa tttatcgccg cgtctttcag gtagtttaat ttctgtgaaa
     2701 tctgacattc catatttttt gttactcact tctgcaggaa gattattttt tagataatcg
     2761 ataaatccaa gtcgcgagta gttatcataa gatgtttctt ctgctatgtg tgtccagtcg
     2821 atatatgatc cgtcaatacc agtttcagcc tgagcacgga tttttccttc cattaacctt
     2881 acgtaatatg gatttactgg aactccccac gcttcaaatc cttcatccgt ctccttgcca
     2941 taaggtgcat aacgataata tggaaatgtt ccatccggtc taatcatcat ccacgttgtc
     3001 ggatcttccg gcggtttggg cccgatatag tcttcaaaat cctgccatcg ctcgttataa
     3061 aattttaagt aattaagctg ttctttcgta ggaatatctt tattggaatt tgaattgccg
     3121 tttaatgaag tggataaata tcgaagtacg atgtagcctt tatcatgata tgattgatta
     3181 aactttttag cctcaaccat tttagtatca aattcagacc gggtgacttc atcaaaattt
     3241 ggtgcaccgc tgccaaaagt tacagtcata ttattctttt caagaaatcc taaatccggc
     3301 ttttggccca gtacccaata cccccaatcc gtattaggct tccaaggctg ttgtgcagaa
     3361 gaataatttg caaaaagaat gaatgtaatt tgaattatta aaaatctgtg tagaccgggt
     3421 ttaatagttg ccattttttc tcttattatt ttaattttat tgttaaatct ttctctccgt
     3481 caaaaggacc caagacaata ctgtatttgt ttccgattga ttgaagggcc acttcagtat
     3541 tatcaaaaaa tatccggctg ataccctgtg gggtgttgaa attcatttga taggaatctg
     3601 cggccgtgct ttttaattta agtgtaattt cattttctgt ctcagaaata tctgaaatat
     3661 ttatagtaga acttagtaat gaaaatttgt ttgatttgat tttcttaagc cttacagacc
     3721 ggtctgtatt tgtataaccg caaatctcat ttggatttat gccgctataa ttgaacccgt
     3781 ctgcattcaa aagcagatag ccttccggcg cattgaaact aaatttagct ttactttttg
     3841 ttaaaacatt tagaattaac ccgtcttcat tcttttctaa cttagtcacc ggcttggata
     3901 tataagtcag atgcggttca acgttagatt catcggctaa tataattttt agatcagcaa
     3961 tagagccgga tatattagga agtattacca gattatcatt aaatgcaaag tgctcgatat
     4021 tattaataaa tactttatca atctttttgg aagaattatt tattctaatc cccatgccgc
     4081 cggtgtaaag atttgcagaa tccagaccaa gattagaaat atcaaattta aaattcaatt
     4141 gattaccatc agctgaccag ctgtaatttg actgcgccat tatgcaaatc ttgtatctca
     4201 aatcttcagg ctcaggacaa taaatattat tatttttaaa tttagttttt atagcgtcgt
     4261 acatagcgat gttattcgta ttaataatac ggtcctgccc gtattgcggc tgtgaagtaa
     4321 ttgagtagtc atgccacatc tgattaaata tcactccccg tttaatatta tcgtacaaat
     4381 gatcaaagat gctttcttca tatgcggtta tttgctgtgt tgtataactc caggtaggat
     4441 cataaaacca ctgataatcc ggtgaaggag tattttccaa tacaacaaag tttttctggt
     4501 cctcgttgaa ccaggtaaaa tttccgaaac cgatcggcat atcctgctca aatccgtgag
     4561 tcatataaaa tccaaatcgt tttgctatct gattataatc actcatatgg attgtgtttc
     4621 cgggattaat aaaacaatcg actttgccga taccgtatcc gtaatccatc atattaaatt
     4681 cgatttcttt tattgcatca tcaagttctt ccttccatct ctgttcattc atattctgat
     4741 caatattatg gaatttgctg tgagtgccta tttgaccacc gacattctct aatttcttgc
     4801 caagcggtgc aatgtcctgc catccggctg tggcagcggt gcttgatacc caggcataaa
     4861 caggaactac acctgtttca tttgcaatat caatcaaata attaattgtc tgaatttgtg
     4921 cattcaggtt tccgcttgca tctgcatcaa gacgaataat agctgtgaga tcggcattag
     4981 agacctgaag agatggaatt gggtcttcga aagagtcata taaagaccag tatactgact
     5041 caatcaataa attataaatt tgatttttat aaaacacctg aggctgaaca ttgcggaaaa
     5101 aagacggcac gcttgaccag gtagcaaaat cgtttatcag gacaattcga tttttagatg
     5161 tttgtctgac agaaaggaat ggataagtct ccttttcatt tgttaactca agcaatattc
     5221 ttccatcatt ttcaaacgtc tgaacattta atcccccagc gatatgctga gtaacaaaat
     5281 ccccttttag ataatttttt gttatgaact gatcattgtc tttaacttta attctgtaat
     5341 ctggatcacc atagaatccg atatacttaa tatctaagag ttgatttaac tttgaatggt
     5401 cccgttcttt cgttttctca tcgaaataac ctatcaaccc atcaatgata agattcccgc
     5461 cttctgaaag atagttttct aatgctttta ccgttgattc ataaacggca tcagataaat
     5521 aggtgcactg tgtaaaaatc agacaatcat atttttttaa gttcatatct tccgataaat
     5581 cttcggcaga aatacagtca tagggcaccc cggcaagatt tgcaactcct ccccacccat
     5641 gggctgacat ctgtgttact ccccagtgat gtgtgaagct tgatttacta ataatgattg
     5701 caacgcgcgg attttcaaaa ttgaattggc aaaaagcgga gtttccaata atcagagaaa
     5761 aaaataatag acttataaac ttattcattc agttatccct taaataatct gaccgtatga
     5821 tgggttaaaa tgatctttcg aaaacttttt ttaaacgatt aaatggaaac cggttctctt
     5881 cccctgttct ttatttattt aaatattaat tatttattac ggctttaatc aataagtatt
     5941 tattaaaact gacattcaat tttggattct taccatcaac atttgaaatg aatttgtcat
     6001 tgacatagtc gataacttta taagctttgt tttctaatcc tcttagttct acctcaccat
     6061 tccatttatc cgcataaaaa gcatagtaca tagtatcccc tttttgaacc gcgtgtgttt
     6121 caggtttatc ataaccgata tcataaagtt cacctctata atcggctttt gataaataat
     6181 tttctttata gatgtcaagc catttttgcc aaatgatttc tttttcgggt gtcagtaaat
     6241 aatttttatg ttttaaccgg tcagtgggat atgtgaattt tgttccgaca attcccccta
     6301 ttcctacagt tgatgcaaaa tcagaagcac tatcgcttaa ttccacatga tcaccaaaat
     6361 atggggcgtt gatacccatt aatgctttga tcgttttacc cttcaatctt atctgccagg
     6421 aggaagccgg atctgaagca actgtctggt tcatgtaatg aagattaaag aatgaataag
     6481 aagtaccgca tggacatatt tgtattaccg catc
//
LOCUS       DASWBY010000197         5294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_82141, whole genome shotgun sequence.
ACCESSION   DASWBY010000197 DASWBY010000000
VERSION     DASWBY010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82141"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..361
                     /locus_tag="VF870_03565"
     CDS             119..361
                     /locus_tag="VF870_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9251291.1"
                     /translation="MSTKLFVGSIPLSFNNEILKKTFEKHGTVLTARVMTDRTSKKSR
                     GFGFVEMENTSDAYEAVKALNELEIEGKSIVVNETR"
     gene            complement(564..1091)
                     /locus_tag="VF870_03570"
     CDS             complement(564..1091)
                     /locus_tag="VF870_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9251292.1"
                     /translation="MKKYLFSLLLILMVTVLVHSQDMDKMSMDSKHESVYNNLLNALT
                     SGNLEMLDKYVAPDFIEHDPGPMMTKKTGIERIKEDFASYHKIFPDMKSKVHNIAVTG
                     DMLFAYITFTGTSAESFMGMPANHKMTMNMVDLVRFKGDKIAEHWGFTSNEDMMKMMP
                     QDKMSQNKMMDKEMK"
     gene            1673..2578
                     /locus_tag="VF870_03575"
     CDS             1673..2578
                     /locus_tag="VF870_03575"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX9251293.1"
                     /translation="MAIPDYQTIMLPLLKFASDQKEHSVHEAVKFLADQFKLSEDDRK
                     EMLPSGQQEVFVNRVGWARTYMKKAGLLESPQRGTFIITERGRKVLTSNPSKVDNKLL
                     TNYEEFKEFKKKKRKVEEEAEVLEFQDKTPEEAFEAAYENLRTELSSDILEHLKKSDP
                     TLFEKIVIEVLVKMGYGGSLRDAGRAIGRSGDEGIDGIIKEDRLGLDIIYVQAKRWDN
                     TVSRPEIQKFAGALQGQRAKKGIFITTSNFSKEAIDFTSKIESKIILIDGDQLADYMI
                     DFNVGVTTTSKYELKKIDLDYFVEE"
     gene            2578..3312
                     /locus_tag="VF870_03580"
     CDS             2578..3312
                     /locus_tag="VF870_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251294.1"
                     /translation="MKSFFPKPSFKIDDIISYAELVAAENANIQKGMNFQIKPEYSVF
                     LMSVRKNAPYADEWDEKTNTLIYEGHDAPINLAKEPKEADQPYTTPKETLTENGKFFT
                     AAQAYKMKLMEIPHRIKVYEKIKDGIWCYKGFFSLIDAKIVFDGVRKVFKFYLQPVEM
                     KAFNKEIILQHTRLIPTSIKVEVWARDKGQCVLCGSKENLHYDHDLPFSKGGTSLAAE
                     NVRVLCMKHNLKKSNKILTILPILFS"
     gene            complement(3454..4005)
                     /locus_tag="VF870_03585"
     CDS             complement(3454..4005)
                     /locus_tag="VF870_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251295.1"
                     /translation="MILTKLRLLFLILISLLFLSSSCEKSTENEPCENTKWDEAIDQI
                     LEVYIGVPKVFCDAPRNTLLSWNAEQMVFTGTIQKFYCGDIASGSFSMNDAFIPKSMS
                     EDQLGKIKIGQAYQFKFENDNDYLLLTGTLTAYFLDDVTWKTKEFSVKIYYKDLKYDI
                     YTVEHYYLYAIPWATVWELVPEY"
     gene            complement(4395..4973)
                     /locus_tag="VF870_03590"
     CDS             complement(4395..4973)
                     /locus_tag="VF870_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251296.1"
                     /translation="MRTLLISLLIGAALFSSGFGQERKGERKWTIDPATGDTVYTESV
                     TISETEDITPRTSMVVINPLKFLLFYNISYFYKINNGVAIGGGFQIPTPRDVTGFGVN
                     AEARFYPKGNNLKGFYFAPNVSYNNLTSGDDQQSITSVGGLVGWQWFPGDQFAMGLGI
                     GVDYYFFSGDELGFDDYDGFAPALRFDIGYAW"
BASE COUNT         1834 a          878 c          945 g         1637 t
ORIGIN      
        1 tattaagcat caaagagcgg ttaaatataa aaatcagttt atggcaggta gtttaattaa
       61 gtaatttaaa tcggttacta atccggcatt ctatttatta ttaaataaga ggtttctatt
      121 gagcaccaaa ttatttgtgg ggtcaatccc gctgtctttt aacaacgaaa tattaaagaa
      181 aacatttgaa aaacatggaa ccgtgttaac agcaagagtt atgacagacc gtacttctaa
      241 aaaatcccgt ggttttggat ttgttgaaat ggagaatact tcagatgcat acgaagcagt
      301 taaagcactg aatgaattag aaattgaagg taaaagtata gtggttaatg aaaccagata
      361 atgagattaa ttttatactg cagtttaaaa aaggcgctat acagcgcctt tgatatttta
      421 aactatttta tttttactct tgttaattcc aacaaaacag gaattaacaa gatgaaataa
      481 tatcactata cttctttgga attgtcacgt cccccgaaac gtgacaatat aattatctca
      541 ataaacaaga gcgcaactga ttgctatttc atttctttat ccatcatttt attctgcgac
      601 atcttatcct gcggcatcat cttcatcata tcttcgttag acgtaaagcc ccagtgctct
      661 gcaattttat ctcctttaaa tcggacaagg tccaccatat tcattgtcat cttatgattt
      721 gccggcatgc ccataaacga ttctgcagag gtgcctgtaa aagttatgta agcaaaaagc
      781 atatcaccgg taacagcaat gttatgaacc ttggatttca tatctgggaa tattttatga
      841 tatgaagcaa agtcttcttt gatacgttca ataccggttt tttttgtcat cataggaccc
      901 ggatcgtgct ctataaaatc cggagcaaca tatttatcca gcatctcaag attgccgctg
      961 gttaatgcat ttaacaagtt gttatagaca gattcatgtt ttgaatccat tgacatctta
     1021 tccatatcct gactatgaac cagaactgta accatcagaa ttaacagcag ggaaaaaaga
     1081 tactttttca taataagcct cctaaaaaat aattaataat tattaaacgg atattaataa
     1141 ccgtccgtgt tctgtagaaa ataaaaatta tttgattaaa atcaactata gataacaagc
     1201 aacaatcggg tgatgtagta aggcattaaa atgtttgtta aagataatta atccgaaatg
     1261 tttgccggta aacccgcccg ggtgatttga agagatgcat tgaagtggat aagtttatct
     1321 tatgaaatta ttcaaaacag tatccggcgg gaaactttta aaaacgatga aaaaagagaa
     1381 gaaatctgaa cgaaagtttt agttttttct tattcctgtt tttcatttta cattgaacat
     1441 tcaacatttt agaaatcatc ttccattaac aattaaagac tgctgattga agacttgtct
     1501 cactgcatat tgcctacctc ttacttttca tgtttccctt ttttcgtctc tcgtctttcc
     1561 tttcacgtct tacgttctta agtttgcatt gagtttatgg gttgtgtttc atcctctttt
     1621 aggatgggga gttggtgaat aacttattta caaataatat tggagtaagg ttatggctat
     1681 tcctgattac caaacaatta tgcttccact tctaaaattt gcttcagacc aaaaagaaca
     1741 ctcggttcac gaagcagtaa aatttcttgc cgatcagttt aagctttctg aagatgatag
     1801 aaaagagatg ttacctagtg gacaacaaga agtttttgtt aatcgtgtag gttgggcaag
     1861 aacttatatg aagaaagctg gattattaga atcgccccaa agaggaactt ttataataac
     1921 agaaagaggc agaaaagttt taacttcaaa tccttcaaaa gttgataata aattgttaac
     1981 taattatgag gagtttaaag aatttaagaa aaagaaacgg aaggtggaag aagaagctga
     2041 ggttttagaa ttccaggata aaacacctga agaagcgttt gaagcggcat acgaaaacct
     2101 taggacagaa ttatcgagcg atatattaga gcatcttaaa aagagcgatc ctactttgtt
     2161 tgaaaaaatt gtaattgaag ttcttgttaa aatgggttat ggtggaagtt tgcgtgatgc
     2221 gggcagagca attgggcgaa gcggagatga aggaatcgat ggaataataa aggaagatag
     2281 gctggggttg gatattattt atgttcaagc aaaaagatgg gacaatacgg ttagtagacc
     2341 agagatacaa aagtttgccg gagcactaca agggcagcga gctaaaaagg gaatttttat
     2401 tacaacgtca aatttttcaa aagaggcaat tgacttcaca tccaaaatcg aatctaaaat
     2461 cattttaatt gatggagacc agttagcaga ctacatgatt gattttaatg ttggcgtaac
     2521 tacaacctca aaatatgaat tgaagaaaat agacttagat tattttgttg aagaataatg
     2581 aaatccttct tcccaaaacc aagttttaaa attgatgata taattagtta tgctgaatta
     2641 gttgctgcgg agaatgctaa catacagaag ggaatgaatt ttcagataaa gccggagtat
     2701 tcggtgtttt taatgtcggt tagaaagaat gctccttatg cagatgagtg ggatgaaaag
     2761 acaaacacgc ttatttacga agggcacgat gcaccgataa atttagcaaa agaaccaaaa
     2821 gaggcggacc agccttacac aacacccaaa gaaacgttaa ctgaaaatgg aaaatttttt
     2881 actgcggctc aggcatacaa gatgaagttg atggagatac cgcacagaat aaaagtttat
     2941 gagaaaataa aagatggtat ttggtgttat aagggtttct tcagtttgat tgatgcaaag
     3001 attgtgtttg atggagtaag aaaggttttc aagttttatc ttcagccagt ggagatgaaa
     3061 gcatttaaca aagaaataat tttacagcat acaagactga ttccaaccag tattaaagtt
     3121 gaggtgtggg caagagataa aggtcagtgt gttttatgcg gctcaaaaga aaacttacat
     3181 tatgatcacg acttaccatt ttcaaaaggc ggaactagtt tggccgctga gaatgtgagg
     3241 gttctttgta tgaagcataa ccttaaaaag tctaataaga ttttgacaat acttccgata
     3301 ttattttctt aaaactttgt gttcttagtg gttaagaccc taaccatcaa tcttaccaaa
     3361 aaaagcagca tattataata taacacaaca ttcagtagct aatctagccg taacaaaaag
     3421 tcatcagtca aatacagctt ttatacacta ttattaatat tcaggtacaa gctcccagac
     3481 agttgcccag ggtattgcat agaggtaata atgttcaaca gtatagatat catacttaag
     3541 atctttataa taaattttaa cagaaaattc tttagttttc cacgtaacat catctaaaaa
     3601 atatgctgta agggtgccgg ttaagagaag atagtcattg tcattttcaa atttaaattg
     3661 ataagcctgt ccaattttta ttttacccag ttggtcttca ctcattgatt taggaatgaa
     3721 agcgtcgttc atactgaatg atccactggc gatatccccg cagtaaaatt tttgtatagt
     3781 accggtgaaa accatttgtt ctgcattcca tgaaagcaaa gtattacgcg gcgcatcgca
     3841 aaaaactttt ggtacgccaa tatatacctc taaaatctgg tctatagcct catcccattt
     3901 agtgttttca caaggttcgt tctccgtaga tttttcgcaa gagctgctta agaaaagtaa
     3961 agagataaga atcaaaaaca atagacgtaa tttggtgagt atcatacgca ccatccttcc
     4021 ctgaaaaaga tttgataaaa agtttagaat cagattaatt agtcatcatc ctgagctaat
     4081 cttattataa gtatcttaac aacagagatc ccctgagcaa agaaatatgg ggtacgaaat
     4141 cattatagtt acgtatcgct attacaattg cagttaaact taaatgaaat attctagaaa
     4201 ctcaattatt gattagcaaa tagtgcctat aagcaaatca tacgtagtaa tgattttttt
     4261 taagatatta agttgcagct ttacgagaat gataggaaaa acatattttt tagttaacca
     4321 accacctcag ctttcagcct tgccctatga atcgggaaga aaagaagagc tgtgtgttaa
     4381 tattatttaa tcatttacca ggcgtaacca atatcaaacc taagtgccgg tgcaaaaccg
     4441 tcataatcat caaacccaag ctcatcaccg gaaaaaaagt aataatcaac acctatacca
     4501 agtcccattg caaactgatc gcccggaaac cactgccagc cgaccaatcc gccaacagac
     4561 gttattgatt gctgatcatc tccgcttgtt aagttattgt aagaaacatt tggcgcaaaa
     4621 taaaaccctt ttaaattatt tcctttgggg taaaatctgg cctcggcatt tactccgaat
     4681 ccggtaacat ctcgcggagt tggtatttga aaacctccgc cgattgcaac gccgttgttt
     4741 attttataaa agtatgaaat attatagaaa agcagaaact ttaacgggtt aattacaacc
     4801 atacttgtac gtggtgtaat gtcttcagtt tcagaaatgg taacagattc ggtatatact
     4861 gtatctcctg ttgcgggatc aattgtccac tttctttcac ctttacgttc ctgcccaaaa
     4921 ccggatgaga acagggcagc cccgattaat aaagatatca acaatgttct cataattatc
     4981 tcctctggaa aattttttat caaatctttt tacttagcac aggacaactt tagtgccgta
     5041 tactgataaa ctaatttgca taattacatt atcaaatata catcaatttt taaataacga
     5101 ttgatacagt aagtgaagat tataaaacac aatacggatg aacattaaat attaaaaatg
     5161 ttcatttacc tgtattggtt aatgttgatt caatttggca gttaatccaa caatccattg
     5221 ctgattttta caattccatg acaacttaaa tctgtttaat atttattatt gtacaggact
     5281 aacagactac tgta
//
LOCUS       DASWBY010000198         5006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_158551, whole genome shotgun sequence.
ACCESSION   DASWBY010000198 DASWBY010000000
VERSION     DASWBY010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158551"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..2288
                     /locus_tag="VF870_03595"
     CDS             378..2288
                     /locus_tag="VF870_03595"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251297.1"
                     /translation="MKKNFTTLLFFLISQTLLFAQNGNPLPFGRTTLFAGSGLCEVCH
                     TSDGTVLTVNGQDVSMVKYWRSTMMANASKDPFWRAVVAEEVHRFPTLQQTIETTCTK
                     CHAPMGYTEAIFNGQSNYSMAQMKADPIANDGVSCTVCHQIKPDNFGTAASYSGHYQI
                     DPDSIIYGPYPDPELNAMIEMAAFLPVYTNHLNQSELCATCHTLFTPYLDNQGQIAGD
                     FPEQTPYLEWKNSIYPAQNTQCQDCHMPMITDSVDIAWIPQSHQVYRTPFWKHSFVGG
                     NVYMLNMLKDNISTLGLTASAENFDSTIARAEKNLTQKAIDLFITTTYENDSLNVFVK
                     IENKTGHKIPGGIPFRRMWIHLKVTDPSDNIIFESGKWNAQGEINGLDAGYEPHYNLI
                     NNEDDVQIYEGVMVDVDNAVTYTLLRASSYIKDNRIPPNGFTTSHPSYDTTAIFGSAV
                     FDPNFNKENITEGTGSDIVNYRLPVTNETTYKVFAEVCFQAIKPRVVDQFASISEPDI
                     TQFIGMYNALPNLPFIMKSDSLSVFVTDVGDDVNNVKGFELSQNYPNPFNPNTQISYQ
                     ISVPSKVSLRVFDVLGNEIVTLVDDYKSIGRYDVNFDASDLSSGLYFYKLEATADNKN
                     VFREVRKMILLK"
     gene            complement(2373..4211)
                     /locus_tag="VF870_03600"
     CDS             complement(2373..4211)
                     /locus_tag="VF870_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX9251298.1"
                     /translation="MKKLFILFVLFASNIFAQYITPNTGVNWNLDDLVTNSGGVVTGA
                     FPNYAISNKISVSASDRIYINPGTTVMFSGSASGFEIYGKFLAVGTVSDSIIFSSLTQ
                     DSTGAAYNGFYFRDTAVDTACIISYARIEYAYYGLRCLEASPTLTNSFLWKCRRGANL
                     SSNSSPNISHNRIERSYEYGITLTTGSSPLIEWNELVNNNSQNTSPKNQISIGTQGNN
                     SPTILNNIIRGGMFNRTGGISISTLIGGSSSSSEIAYNEIFNNSFGIAMTGSNPITCY
                     VHDNLIYNNNINPDVMVAGSGINTYGTAVVAPIITKNTIYGNWWGVTIQVGIAGQPGA
                     NPNLGNIENADTTDNGLNIIYGNIQDTIVYDLYNNTPDPIYAQNNDWGVYDSVSIEDH
                     IFHFVDDSTLGQVYFMPFSNSIPVELTAFSANVLDGNVVLNWITATELNNSGFEIQKG
                     SYTSTSLSVTEWEKIGLVSGNGTTTAKSFYSFVDKNPSQGKSYYRLKQIDFDGSYEFS
                     NIVEVDFSIPIKFSLEQNYPNPFNPSTKIKYTIPGITRNGVEGSRVILKVFGVLGNEV
                     ATLVNEEKPAGTYEIDFDASKLSSGVYLYELRAGDFVDMKKMILLK"
     gene            4458..>5006
                     /locus_tag="VF870_03605"
     CDS             4458..>5006
                     /locus_tag="VF870_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251299.1"
                     /translation="MKHKIFTLFLLMFFFSFLTVPVLAQVRTGLINDLDEFFPISEIV
                     AESNSYSDLFDPGFIVYPGFPQQGLYSVISPKTGAIYCNLDAEPEMEIIFGAGETLYA
                     VNLDGSAVTGWPKTFTQYYEAVWTVSFGDIDGDGEGEVVAGIGGPLGGHIQAFHKDGS
                     IVTGFPVNVGKYPMSPSLADLDG"
BASE COUNT         1634 a          854 c          855 g         1663 t
ORIGIN      
        1 atcttttttc tgttgtcgcc tctaaaatta atatcgatca caaaaaagtt tagcttttct
       61 gctatttcat tacaaattcg aactatattt tctttattta tatccatact aacaaaaaac
      121 gccctcaacg gggcgaatca ctaagccaaa aataggatta attattatga caaacaagaa
      181 aattgtaagc ccgtttttgt ggtaaacatt aaaaaccctt cgaattgatc aaaaataaaa
      241 gtgatggatg ctttaacatg aactaactcc aatattttta acctcaaaat aatgtgtgca
      301 aattcaatta taatcactta ttttaaaaga gaaaatcacc ttttaaaaaa atttgaaaag
      361 aaaatccgga ttccaaaatg aaaaaaaatt ttaccacttt acttttcttt ttaatcagtc
      421 aaacactttt atttgcacaa aatggcaatc cattaccatt tggcagaaca acactttttg
      481 caggttctgg cttgtgcgaa gtttgccaca caagtgatgg cactgtttta actgtaaatg
      541 gccaggatgt ttcaatggtt aaatattggc gctcaacaat gatggccaat gcaagtaaag
      601 atcctttctg gcgtgcggtt gtggctgaag aggttcatag atttccaact ttgcagcaaa
      661 cgattgaaac tacctgtaca aaatgtcatg ctccaatggg ctacacagaa gcaattttta
      721 atggtcagtc taattattca atggcacaaa tgaaagctga tccaattgcg aatgatggag
      781 taagctgtac tgtttgtcat caaataaaac cggataattt tgggacggct gcttcatact
      841 cgggtcatta tcagattgat cctgatagta taatttatgg gccatatccg gatcccgaat
      901 taaatgcaat gatagaaatg gcagcgttct tacccgttta tacaaatcat cttaatcaat
      961 ctgagttgtg tgcaacctgt catactttgt ttactcctta tttggataat caggggcaga
     1021 ttgccggaga ttttccagaa caaactccgt acctcgaatg gaagaacagt atttatcctg
     1081 ctcaaaacac acagtgccag gattgccata tgccaatgat aactgattct gttgatatcg
     1141 catggattcc acaatcacac caggtttacc gcacaccttt ctggaagcat tcttttgttg
     1201 gcggaaatgt ttatatgctg aatatgttga aggataatat ctctacgtta ggattaacag
     1261 cttcggcgga aaattttgat tctacaattg caagagctga aaaaaatcta acacaaaaag
     1321 cgattgatct ttttatcact acaacttatg aaaatgattc attaaatgtt tttgtaaaga
     1381 ttgagaataa aacaggacat aaaattcccg gtggaattcc attccgtaga atgtggatac
     1441 atttaaaagt aactgatcca tctgataata ttatttttga atccggtaag tggaatgcac
     1501 aaggagagat aaacggattg gatgccgggt atgaacctca ttataatctt ataaacaatg
     1561 aggatgatgt tcagatttat gaaggtgtta tggttgatgt tgataatgca gtaacttata
     1621 ctttgttgcg tgcttcatct tatataaaag ataatcgcat tccaccaaat ggatttacaa
     1681 cctcacatcc aagttacgat acaacagcaa tttttggcag tgctgtgttt gatcctaact
     1741 ttaataaaga aaatattaca gaaggtaccg gaagcgatat tgtcaattat agattacctg
     1801 taaccaatga aacaacctac aaagtttttg cggaagtttg cttccaggca ataaaaccaa
     1861 gggttgttga tcagtttgca agcataagcg aaccggatat aactcaattt attgggatgt
     1921 acaatgcact tcctaattta ccatttataa tgaaatcaga ttcacttagt gtttttgtta
     1981 cagatgttgg ggatgatgtt aataatgtaa aaggatttga gttatcacag aattatccaa
     2041 atccttttaa tccaaacacg caaattagtt atcagatttc tgttccaagc aaagttagtt
     2101 taagagtgtt tgatgttctc ggtaatgaaa ttgtaacgct tgttgatgat tacaaatcta
     2161 ttggaagata tgatgtaaac ttcgatgctt ctgatttaag cagtggattg tatttctata
     2221 agcttgaggc cacagctgac aataaaaatg tttttagaga agtaagaaag atgatacttc
     2281 tgaagtagtt ggaaatacat ataaacgatt aaaatcttgc agccgataaa aattaatcag
     2341 agacgggtta ggccccgtct ctgattccat tgttatttta gtaaaatcat ttttttcata
     2401 tcaacaaaat ctcccgcccg caattcataa aggtaaactc cgctggaaag ttttgaagca
     2461 tcaaaatcta tttcataagt tccagccggt ttttcttcat taaccaaagt tgcaacttca
     2521 ttgcctagaa ctccaaaaac tttaagaatc actcgagatc cttcaactcc gttccgggtg
     2581 atacctggaa tagtgtattt tatttttgta gatggattaa atggattcgg ataattttgt
     2641 tccagtgaga attttatggg tatactaaaa tcaacttcaa caatgtttga aaattcatat
     2701 gatccatcaa aatcaatttg ctttaatctg taatatgatt ttccctggct tggattttta
     2761 tcaacaaaag aataaaagga ttttgctgtt gtcgttccgt tgccgcttac taaaccaatt
     2821 ttttcccatt ccgtcacact gagcgaagtt gaagtgtaac ttcccttttg tatttcaaaa
     2881 ccagagttat ttaattctgt tgctgttatc caatttagta ctacatttcc atctaaaaca
     2941 tttgcactga atgctgtcag ttcgactgga attgaatttg aaaatggcat aaagtaaacc
     3001 tgaccaagag ttgaatcatc aacaaagtga aaaatatgat cctcaattga tacagaatca
     3061 taaacacccc aatcattgtt ctgtgcatag attggatcag gtgtattgtt ataaagatca
     3121 taaacaatgg tatcctgaat attaccataa ataatattca aaccattatc cgttgtatca
     3181 gcattctcaa tgtttccaag atttggattt gcgcctggct gaccagcaat accaacctga
     3241 atagtgactc cccaccaatt accataaatt gtgtttttag taattatagg agcaactaca
     3301 gcagttccgt aagtatttat accacttccc gcaaccatta catccggatt gatattatta
     3361 ttatagatta aattatcatg tacgtaacag gtaattggat tacttcctgt cattgctata
     3421 ccaaaactgt tgttaaatat ttcgttgtat gcaatttcag aggaagagga ggatccgcca
     3481 attaatgttg atatgcttat tccgcctgtt ctgttaaaca ttccaccacg gattatatta
     3541 tttaaaattg ttggtgaatt attcccttgt gttccgatag atatctgatt tttggggctc
     3601 gtgttttgtg aattgttatt cactaattca ttccattcaa ttaatggtga tgaacctgtg
     3661 gttaatgtaa ttccatattc ataacttctt tcaattctat tatgtgaaat atttggactg
     3721 gaattgctag ataaatttgc acctcttctg catttccata aaaaagaatt ggttaatgta
     3781 ggacttgcct caagacaacg taatccataa taagcatact caattcttgc atagcttata
     3841 atacaagctg tatcaacggc agtatctcta aaataaaatc cgttatatgc tgccccggtt
     3901 gaatcttgtg ttaatgaaga gaagatgatg gaatctgaaa cagtaccaac ggctaaaaac
     3961 ttaccataaa tttcaaaccc tgaagctgac cctgaaaaca taacagtagt gccgggattt
     4021 atgtaaattc tatcacttgc agatacgctt atcttattac taatggcata attagggaat
     4081 gcacctgtta caacaccacc ggaatttgta accagatcat caagattcca gtttactccc
     4141 gtgtttggtg ttatgtactg cgcaaaaata ttagatgcaa agagaacaaa aagtataaat
     4201 agttttttca ctatgagctc cgacttttta attaaaataa aattcaaaaa aaaattagct
     4261 attatatttt atctagtcaa tcaaaatttt ttatatatca tttatatctc aaaatattat
     4321 ttgaagattg ttaagtattg tcaaatgtaa ttcaaacata taaatctttt atttgtattt
     4381 ttaatgagcc gtttagcaaa aaacgcaaat caatactaaa ttgattaaag taaccttatt
     4441 tattgaaagg taatattatg aaacacaaaa tttttactct ttttttgcta atgtttttct
     4501 tttctttttt aactgtacca gttttagcac aagtacgaac aggacttatt aatgatctgg
     4561 atgaattttt cccaatttca gaaattgtgg ctgaatcaaa tagctatagt gatttgtttg
     4621 atcctggttt tatagtttat cccggatttc cacaacaggg attatattct gtaatttcac
     4681 ccaaaaccgg cgcaatctat tgcaatcttg atgctgagcc cgaaatggaa ataatatttg
     4741 gtgctggtga aacattgtac gcagtaaatc ttgatggctc agctgtaaca ggatggccaa
     4801 aaacttttac acaatattat gaagctgttt ggacagtttc ttttggagat attgatggcg
     4861 atggtgaggg cgaagtagtt gcgggaattg gcggtccgct tggtggacac atccaggcat
     4921 ttcataaaga tggttctatc gttacaggct ttcctgttaa tgtgggaaaa tatcctatgt
     4981 ctccatcgct tgcagattta gatggt
//
LOCUS       DASWBY010000199         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_685884, whole genome shotgun sequence.
ACCESSION   DASWBY010000199 DASWBY010000000
VERSION     DASWBY010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685884"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VF870_03610"
     CDS             complement(<1..1077)
                     /locus_tag="VF870_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9251300.1"
                     /translation="MTNEVKLGDKPDLKKDFPVLSFDDWKKQVEKDLKGESFDKKLTT
                     KTYEEINLQPLYTSNDIKDLPQIDNFPGFQNYLRGNSAAGFIGREWEIAQEYNHALPE
                     DLNEALRNDLQRGLNSINIVLDHPTMLGLDADQAKAGQVGRNGLSISGVKKMHILFKD
                     IDIREVPINISCGFSALPFTILFTSYVQEIRRSLMNIKGSIISDPYDYLVTYGKLPIN
                     LSQIFDEIKLATELMIISNSPIKTIGVSGFSYHNSGASAVQELAFSLATGVEYLHKIS
                     GKGLKINEAAKRMKFTFGVGSFYFMEIAKLRAARMLWSKILEEFDVNEENRKMFIHAK
                     TSKLNQTYFDPYVNMLRTTTEAFSA"
     gene            1246..1473
                     /locus_tag="VF870_03615"
     CDS             1246..1473
                     /locus_tag="VF870_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251301.1"
                     /translation="MKPFLSKIFYLISILGFVYSFYNAGLVVNSLFRKSREISSDSIY
                     QEAYGLIYIAAGSFAAAFIFYLISRKISRKS"
BASE COUNT          474 a          258 c          238 g          547 t
ORIGIN      
        1 tgctgaaaat gcttcggttg ttgtacgaag catattcaca taaggatcaa aataagtttg
       61 gtttaatttt gaagtttttg cgtgaataaa catttttctg ttttcttcat taacatcaaa
      121 ctcttcaaga attttactcc ataacatcct tgctgctcta agtttagcaa tttccataaa
      181 ataaaatgat cccacgccaa aagtgaactt cattcttttt gctgcttcat taatctttaa
      241 tcctttgccg gaaattttgt gtaaatattc aacaccggta gctaaagaga aggcaagttc
      301 ctgaacagca cttgcaccgg aattatgata tgaaaatccg cttacaccaa ttgtctttat
      361 tggtgaattt gatataatca ttaactcagt agctaattta atttcatcaa atatctgact
      421 taaattaata ggcagctttc catatgtaac taaataatcg taaggatcgg aaataattga
      481 tccttttata ttcattagac ttcttcttat ttcctggaca taagaagtaa ataaaatcgt
      541 aaaaggcaat gcggaaaaac cacagcttat atttattggc acttctctaa tatcaatatc
      601 tttgaagaga atgtgcattt tttttacacc tgatattgat aggccgtttc tgccaacctg
      661 cccggctttt gcctgatcag catcaagacc taacatggtg ggatgatcta aaactatatt
      721 aattgaattt aatccgcgtt gcagatcatt tcttaatgct tcattcaaat cctcaggcag
      781 agcatgattg tattcctgag caatttccca ctcacgcccg ataaatccgg cagcagaatt
      841 cccccgaagg taattttgaa aaccagggaa attgtctatt tgaggaagat cttttatatc
      901 atttgatgtg taaagtggtt gaagatttat ttcttcgtaa gtttttgtgg taagcttttt
      961 atcgaatgat tcacctttaa gatctttttc gacttgcttt ttccaatcat caaaacttaa
     1021 aactggaaaa tcttttttca gatctggttt atcaccaagt ttaacttcat ttgtcataaa
     1081 ttgactccgg aattcataaa aacaactatg taaaattact aaaaagttat tgcatttttt
     1141 tgtatcgttt gtaaataact attttgtaaa ttgttgcttc ataaaagtaa ctgctcgttt
     1201 aacttaaaat tatttaaaaa tcattgtagg cgatggataa aacggatgaa accattctta
     1261 tcaaaaatat tctatttaat ttcaattctc ggtttcgtgt attcatttta taatgcggga
     1321 ttagttgtca attcgctttt cagaaaaagc agggaaattt cttcggattc aatttatcaa
     1381 gaggcctatg gattaatata tattgccgca ggtagttttg ctgcggcatt tattttttac
     1441 ctgattagta gaaaaatttc caggaaatcc tgagtgaact atgcaattac aatttatgcc
     1501 ggcggattcc aggtttc
//
LOCUS       DASWBY010000200         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_9296, whole genome shotgun sequence.
ACCESSION   DASWBY010000200 DASWBY010000000
VERSION     DASWBY010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9296"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VF870_03620"
     CDS             <1..437
                     /locus_tag="VF870_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251302.1"
                     /translation="EGMIESKEVETPIQPNAVFEERSEMKSKAVDQLLKDEMVSGRSD
                     SDNLKSGVTENFGGSAAPSATTLASEINKADSFEREMKRERDEIVNLPVVKSDADNLS
                     KENINFMQRNLSTEEKKEVQQLKMKVQAEKGSKTQQNSNKLP"
     gene            516..764
                     /locus_tag="VF870_03625"
     CDS             516..764
                     /locus_tag="VF870_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEX9251303.1"
                     /translation="MKKGIHPEYKPVVFQDASCDFAILTRSTIRTSDTIKWTDGNTYP
                     LVKLEISSGSHPFFTGKQKLLDTAGRVEKFNKKYSKKQ"
     gene            868..1938
                     /locus_tag="VF870_03630"
     CDS             868..1938
                     /locus_tag="VF870_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9251304.1"
                     /translation="MNKNTKKVLIGITIVVLLAIVFLPKILSSAEDGSNVMAGGRPDI
                     AVPVKAHIVSLETLSNSVFTTGTILANEEVELRSEISGKIVKILFKEGSFVNKGDLLI
                     KINDADLQAQLRKAESKVKLSVEKELRQRQLRDGNLISQEEYDNTISELNVNQADYDL
                     IKAQIDKTEIRAPFSGVVGLRSVSEGSYVTTSTVMARLQNFNSLKVDFSIPERYSASV
                     KTGDDLEFKISGSSNVFKAKVYAIEPKIDPGTRTLQIRAICSSAYKELIPGAFANVEL
                     NLKQTSNAILIPTVSIVPELKGQKVYIYKSGIVVPQNVELGTREETRVQIISGLSLGD
                     TVITSGILQIRPNSKVNISEFN"
     gene            1953..>3679
                     /locus_tag="VF870_03635"
     CDS             1953..>3679
                     /locus_tag="VF870_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9251305.1"
                     /translation="MSLASISIQRPVLAIVMSIVIIVFGILGYTYLGVREFPSVDPPI
                     ITVSTNYVGANADVMESQVTEPLEESINGIAGIKSLTSVSRDGRSTVTVEFDIDVDLE
                     AAANDVRDRVSRSLFNLPPDIDIPIVAKADADATPIVFLNVQSDTRNLLELSEIANNV
                     FKERFQTISGVSSVQIWGEKKYAMRLWLDPAKLASFQLSPVDVRNALNRENVELPSGR
                     IEGDETELSIRTIGRLQTEEEFNNLIIKESAGSIVRVRDVGRAELGAENERSILKRDG
                     IPMVGVVLIAQAGANNIAIADEFYKRLEQLKKTLPADIQTGIGFDVTTYIRESIKEVE
                     QTILVAFLLVVLIIFLFLRDWRTTLIPMLVIPISLIGSFFIMYIADFSINVLTLLGIV
                     LAIGMVVDDAIVVLENIYTKIENKMDPIEAGKKGSAEIFFAVISTTVALASVFMPVIF
                     LQGLTGRLFREFGIVVAGAVIISSFVALTLTPMLSAKILKRREHHNWFYNLTEPFFVA
                     MNATYKRQLTYFISHRWLAFPVTAAAMIIIYVLFATLPTELAPLEDRSRLSITATAPE
                     GATFEYMDR"
BASE COUNT         1259 a          592 c          725 g         1103 t
ORIGIN      
        1 atgaggggat gatcgaatca aaagaagttg agacaccaat tcagccaaat gctgtttttg
       61 aagaacgatc tgaaatgaaa tccaaagcag tcgatcaatt attaaaggat gagatggtct
      121 ctggcagatc cgactctgat aatttgaaat ctggagtgac agaaaatttt ggtggaagtg
      181 cagcgccatc ggccacaact ttggcatctg aaataaacaa agcggatagt tttgaaagag
      241 agatgaaacg agaacgcgat gaaattgtaa atcttccagt tgtaaaaagt gatgcagata
      301 atctaagtaa ggaaaatatc aactttatgc agagaaatct ctcaacagag gagaaaaaag
      361 aagttcagca gttaaagatg aaagtacaag ccgaaaaagg ttcaaaaaca caacaaaatt
      421 ccaataaatt gccctaaaag gtatattgga taatactaat gaaacgtcta tatttacagc
      481 cacgatttta agaaaaacca ttatttagga tgtttatgaa aaaaggaatt cacccagaat
      541 ataaaccagt tgtatttcaa gatgcatctt gtgattttgc aattttaact cgctctacga
      601 ttagaaccag cgatactatt aaatggacag atggaaacac atatccttta gttaaattgg
      661 aaatttcaag cggatctcat cctttcttta ccggaaaaca aaaactactt gatactgccg
      721 gtagagttga aaaattcaac aagaaataca gcaagaaaca atagcaatat attttgagtt
      781 tagatttatt tttagcctcg acttttcggg gcttttttat ttttgacttt aaagaaacta
      841 agcgtttgat taggttgttt tataattatg aataaaaaca caaaaaaagt acttatcgga
      901 attacaattg tagttctgct tgcaattgtt tttttaccta aaatattatc ttctgctgaa
      961 gacggaagta atgttatggc gggaggcaga ccggatatcg ctgttccggt taaggcacat
     1021 attgtttctt tagaaacatt aagcaatagt gtttttacta caggaacaat tcttgccaat
     1081 gaggaagttg aactacgaag tgaaatttca ggtaaaattg ttaaaatact ttttaaagaa
     1141 gggtcattcg taaataaagg agatctttta ataaagataa acgatgctga cttacaagcc
     1201 caattacgaa aagccgaatc caaagtaaaa ttgtcagtag aaaaagaatt aagacaaaga
     1261 cagctgcgtg atggaaattt gattagccag gaagaatatg ataatactat tagcgagtta
     1321 aatgttaatc aagctgatta tgatttaatt aaagcacaaa ttgataagac tgaaataaga
     1381 gcaccattta gcggtgttgt tggattgcga tccgttagcg aaggaagtta tgtaacaact
     1441 tctaccgtga tggctcggtt acaaaatttt aacagcttaa aagttgattt ctcaattcct
     1501 gaaagatatt ctgcatctgt taaaaccggc gatgaccttg aatttaagat tagcgggagc
     1561 agtaatgttt ttaaagcgaa ggtttatgca atagaaccca aaatagatcc agggacaaga
     1621 accttacaaa taagagctat ttgtagttct gcttacaaag agttaatccc tggtgctttt
     1681 gcaaatgttg aattaaattt aaaacaaacc agcaacgcta tacttatacc tacagtttct
     1741 attgtgccgg agttaaaagg acaaaaagtt tatatttata aaagcggcat tgttgttccg
     1801 caaaacgttg aacttggcac cagagaggaa acccgcgttc aaataatttc cggtttaagt
     1861 ttaggagata ctgtaattac ttcaggtatc ctccaaatca ggccgaactc taaagtaaat
     1921 atttcagaat ttaattagta gtgaattcct aaatgagttt agcatccata agtattcaaa
     1981 gaccagtact tgcaattgta atgtcaattg taattattgt gtttggaatt cttggttata
     2041 cttatcttgg cgttcgtgaa tttccatccg ttgatccacc aataattaca gtttcaacca
     2101 attatgttgg cgcaaatgca gatgtaatgg aaagtcaggt tacagaacca cttgaagaat
     2161 caataaacgg cattgcagga attaaatctc ttacttctgt tagtcgtgat ggaagaagca
     2221 cagttacggt tgagttcgat atagatgttg atcttgaagc agccgcaaat gatgttcgtg
     2281 atagagtttc aagatcactt tttaacctgc ctcccgatat tgatatccca atagtagcca
     2341 aggctgatgc cgatgcaaca ccaattgtat tcctaaacgt tcaaagtgat acaagaaatt
     2401 tgcttgaact ttctgaaata gctaataacg tttttaaaga aagatttcaa accatcagcg
     2461 gtgtaagcag cgtgcagatt tggggagaaa aaaaatatgc aatgcgtttg tggttagatc
     2521 ctgcaaaact tgcatcattt caattatcac cagtcgatgt taggaacgcg cttaaccgag
     2581 aaaacgttga actgccttcc ggcagaatag aaggagatga aacagagctt tcaataagaa
     2641 caataggcag acttcaaacc gaagaagaat ttaacaattt aattattaaa gaatctgccg
     2701 gcagcatagt tagagtcaga gatgttggga gggcagagtt aggtgctgaa aatgaacgct
     2761 caattttaaa gcgcgatgga attccaatgg ttggcgttgt gcttatcgca caagcaggtg
     2821 caaacaatat tgcaatagct gatgaatttt ataaaagatt agaacagcta aagaaaactt
     2881 tacctgcaga tattcaaact ggtatcgggt ttgatgttac aacctatatt cgggaatcca
     2941 taaaagaagt tgagcaaact attcttgttg cattcctgct ggttgtgctt ataatatttt
     3001 tatttctgcg tgattggcga acaactctta ttcccatgct tgtaattcct atttctctta
     3061 taggatcatt ttttataatg tacatagccg atttttccat caatgtgtta acgctgcttg
     3121 gtattgtgct ggcaattgga atggttgtgg atgatgcaat agttgtttta gaaaatattt
     3181 acacaaaaat tgaaaataaa atggatccca ttgaagcagg caaaaaggga tctgctgaaa
     3241 tattttttgc agttatatct acaacggtgg ctcttgcatc cgtttttatg cccgtgattt
     3301 ttctgcaggg attgaccgga agacttttta gagaattcgg aattgtagta gcgggtgcag
     3361 ttataatctc atcttttgtt gcacttactt taacaccaat gctcagtgca aaaattctta
     3421 aaagaagaga acatcataat tggttttaca atctcacaga accattcttt gtagctatga
     3481 atgccacata caagagacaa ttaacttatt ttatatcgca tcggtggtta gcatttccag
     3541 tgactgccgc ggccatgatt attatttacg ttctttttgc aactctgcca acagaacttg
     3601 ccccgcttga agacagaagt agattatcta ttacggcaac ggcaccggaa ggcgcaactt
     3661 ttgaatatat ggatagata
//
LOCUS       DASWBY010000201         6288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_646193, whole genome shotgun sequence.
ACCESSION   DASWBY010000201 DASWBY010000000
VERSION     DASWBY010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646193"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /gene="menD"
                     /locus_tag="VF870_03640"
     CDS             complement(<1..1200)
                     /gene="menD"
                     /locus_tag="VF870_03640"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX9251306.1"
                     /translation="MLIKINRNYFWGNIFFDQLAECGVKYACVSPGSRSTPLAYALSQ
                     NKRIKSFVIIDERTSGFFAVGIAKQTNSPVIVVTTSGTAAAELYPSIIEAYQNRVPLI
                     ICTADRPASLRNTGANQTINQDNLYNNHTRLFYDTGLPKITLKKVEQLKDTAKNIFDI
                     CSSKNVGPVHINIPFEKPLEPNSFTDEIEEKILVESLPDEISEDTNKSSKSFEKKDIS
                     ILTDMLRKSKTGLITIGPGNFSDSFFSNLENFSIKFSLPVFADASSALRFRSTPLSNL
                     ITNYDALVRSDFFAKLFTPKFILHFGKAFTSPKLEDYYSAVKPKGFAINKFGDTHNSS
                     RRFKVIKSDEELFLEDLVNSADLKQNDISPELKKLKNIDSEIEKLKPEIFSIAKKINE
                     TGVMLNII"
     gene            complement(1184..2587)
                     /locus_tag="VF870_03645"
     CDS             complement(1184..2587)
                     /locus_tag="VF870_03645"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEX9251307.1"
                     /translation="MSNTAQISANTSNDLSENLKDPKKVFFSYAAKISNLKFTLLLEH
                     FTNIAEDVFYYSEPESNISFLSFEILNKQSFSFDNYESLAEEISILKNKLVSNHSEYE
                     KFNLPIFITSVKFPIKKDSEEWSDFNDIDFIVPKIILFQHSGSCFIIANFFSESFSHN
                     ENFNEFLERETELIYNLESRLQEGSGERTTIKNTSDGYDFPTWKNKIESVVDEIKHEN
                     IDKVVIARRVESEITSPINWNKTIGDLNLKFPTCTNFLYKSGKSIFFGTTPEILIKYS
                     GNSFSTEALAGSIMRGIDPEQDKQLEEELLKSDKNKTEHEAVIDYIKKSVFNFVDNVE
                     IVENPIVKKLPNIQHLQTSVTGTLKSKDQIFKIISSLFPTPAVCGIPKEQSLKLIEKT
                     ENFDRGLFSGVIGWFNADNYGEFFVTIRSALVKDNKLYAYAGCGIVEGSDPQEEFDET
                     KLKLKPILSLFNNADKN"
     gene            complement(2650..3597)
                     /gene="rsgA"
                     /locus_tag="VF870_03650"
     CDS             complement(2650..3597)
                     /gene="rsgA"
                     /locus_tag="VF870_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX9251308.1"
                     /translation="MISRSESKDFYIFSDADHSLIRCSLKGKFKKDFNLKKDKLFNTD
                     FAAVGDYVLYDLNDDGTGVIYEILPRKNYLSRKAIKTRGASFRGERLEQIIASNIDNI
                     FIVTSTLEPDFNNKTLDRFLVACESAHIKTSIIINKTDLADESSVKPWADLYKKIGYK
                     VFLTSTINSTGLDYLFKSLKGKKNLFWGQSGVGKSSLLNLFYPDLNLKVGEISNYTSK
                     GTHTTSTSVMIFVGEKTYIIDTPGVREIDPFGIRKEDLGHYFKEFENFITSCRFNTCT
                     HHHEPGCAVIDAVENGNISEERYDSYLRILDTVEKDMNF"
     gene            complement(3686..5143)
                     /locus_tag="VF870_03655"
     CDS             complement(3686..5143)
                     /locus_tag="VF870_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251309.1"
                     /translation="MFTKIIVRIFFIANFLTISGIAQNYNDAIRVGFPGLGPDARALG
                     MGNAFVAVGDNAAGTFFNPAGLALIKSSMLTAGFDFSNFNNDVSFLGNESSSKTNSTK
                     FNQASIVLPFPTKRGSMVVGIGYNQSKDLSGILEFNGFNSGNTSWINYFSNQNSDIPY
                     DLKLSYPLYDQNNNYIKDTTNILGSLNQSGTILNTGAINNWDFSGAIEIAKNTFIGGS
                     FTIHTGSFESNNDYYEDDTQKYYEGITAVGEPQTEDFRTFNLKNLLQWDIGGYSGKLG
                     MMYQWESLMRLGLTIQFPKIYTIEENFNVNGSSEFGTGQKYFLDTYKYSDKVKYDIQT
                     PYEFTAGAAFNLSFVIISGQINIIDYTQMEFKNPEGIDPAYFADRNSDIKKFLRVAYN
                     ANLGAELTLPLVPIKLRAGGIYQQSPFEADDASFDRKYLTAGVGFNFEGVFGIDIAYA
                     YGWWKDIADNYDVNVSRTYQDITQQTVMLTTTFRF"
     gene            complement(5149..5586)
                     /locus_tag="VF870_03660"
     CDS             complement(5149..5586)
                     /locus_tag="VF870_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251310.1"
                     /translation="MKKLISIIAVFSVMTLLQGCYTILDLPESDSSGYVTTTEYAPVI
                     IYEPIPVPPPPTYYPPSNGYDQPVKGTNPPQKERTNDNGNGNNNIRNDGNGRGSSGRD
                     TDFTSNNVTRSESYSSSNNNSSSTSNNNNRGSSDTRNSGNGRR"
     gene            complement(5592..>6288)
                     /locus_tag="VF870_03665"
     CDS             complement(5592..>6288)
                     /locus_tag="VF870_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251311.1"
                     /translation="YYYYPTYWYPNYCYGYYDSYPYYDNGYYDTYKYRTNDGYKIRNN
                     SGGRNSGGDRNLYTSTDGTSTLRDQLKTREKQIVSGRDDKNRELTGLRTSVRDTKSRE
                     TTTRTLDAINTRTTETMRKNNTVKDSRMNDVINRTDTRKNTNNTRVTDPNRKSTDKKN
                     PNTIRDNKTNVNKRPETNTNKSPNRNYNPPKSNNNTPRSYSPPSGRTSSPPRGNTNSN
                     PPRNSGSNNSGRR"
BASE COUNT         1942 a         1064 c          984 g         2298 t
ORIGIN      
        1 aataatattc agcataacac cagtctcatt aatctttttt gcgattgaaa atatttccgg
       61 tttaagcttc tcaatttctg aatctatatt ttttagtttt tttaattccg gtgaaatatc
      121 attttgtttt agatctgctg aattgacaag atcttccagg aacaactctt catctgattt
      181 tatgacttta aatctacggg aagaattatg cgtatctcca aatttattaa ttgcaaaacc
      241 ttttggttta acagctgaat agtaatcctc cagtttggga gatgtaaacg ctttaccaaa
      301 atgcaaaata aatttgggag taaataattt tgcaaagaaa tcagatctta caagagcatc
      361 gtaattagta attaagttag ataagggagt tgatctaaaa cgtaatgcgc ttgaggcatc
      421 agcaaaaaca ggcaaagaaa atttaataga aaaattttca aggttagaaa agaatgaatc
      481 actaaaattg cctggtccaa ttgttatcaa accggtttta cttttacgaa gcatatcagt
      541 gagtatgctg atatcttttt tttcaaaact tttggaagac ttattagtat cttcagaaat
      601 ttcatccggt aaagattcaa ccagaatttt ttcttcaatc tcatctgtaa aagaattggg
      661 ttcaagcggt ttttcaaatg gaatattaat atggacagga ccaacatttt tgctgctgca
      721 gatatcaaaa atattttttg cggtgtcttt aagttgttca accttcttta gagtaatttt
      781 tggtaagccg gtatcataaa ataatcttgt atgattatta taaagattat cctggtttat
      841 tgtttgattg gctcccgtat ttcttaaaga agcaggtcta tcggcagtgc agataattaa
      901 gggaactctg ttttgatacg cttcaattat tgaaggatat aattctgcag ccgcagttcc
      961 ggaagtagta acaacaatta caggtgagtt ggtttgtttt gcaattccta ctgcaaaaaa
     1021 cccggatgtt ctttcatcaa taataacgaa ggattttatt cttttatttt gtgataaggc
     1081 gtaagcaagc ggagtgcttc ttgaacctgg agaaacacag gcatatttaa caccgcattc
     1141 agcaagctga tcaaaaaaaa tatttcccca aaaataatta cgattaattt ttatcagcat
     1201 tattaaataa ggatagaatt ggttttaatt tcaatttagt ctcatcaaat tcttcttgtg
     1261 gatcagagcc ttcaacaatt ccacagcctg cgtaagcata tagtttatta tcctttacaa
     1321 gtgcagaacg gatcgtcacg aaaaattcgc cgtaattatc agcattaaac catccaatta
     1381 caccagaaaa taatcctcga tcaaaatttt cagttttctc tataagtttt agagattgct
     1441 ctttgggaat accgcaaact gcaggagttg ggaacaacga tgatataatt ttaaatatct
     1501 gatcttttga ttttaatgtg cctgtaactg atgtctgcaa atgctgaatg tttgggagct
     1561 ttttaacgat aggattttct acaatttcaa cattgtcaac aaagttaaag actgattttt
     1621 ttatgtaatc tatcactgct tcatgttcag ttttattttt atcactttta agcaactcct
     1681 cttctaattg cttatcctgt tcaggatcaa tgcctcgcat aatagatcct gccagtgctt
     1741 ctgttgaaaa gctgttaccc gaatatttaa ttagtatttc gggagttgtt ccaaagaaaa
     1801 ttgattttcc tgatttatat agaaaatttg tgcaggttgg aaattttagg tttaaatcac
     1861 cgattgtttt attccaattg ataggtgaag taatttcact ttcaactctt cttgcgatta
     1921 caactttatc aatattttcg tgttttattt catcaacaac tgattcaatt ttatttttcc
     1981 aagttgggaa atcgtaacca tcactagtat ttttaatggt tgttcgttcg cccgagcctt
     2041 cctgcaatcg gctttcaaga ttataaatca attcggtttc tcgttcaaga aattcgttaa
     2101 aattttcgtt atgagaaaaa ctttccgaga aaaaatttgc tattataaaa caagatcctg
     2161 aatgctgaaa taaaattatt tttgggacta taaaatctat atcattaaaa tcagaccact
     2221 cttctgaatc tttttttatc gggaacttta cggatgtgat aaagatagga agattaaatt
     2281 tttcatactc actatgattg gacacaagtt tattctttaa tattgatatt tcttctgcaa
     2341 gtgattcata attatcaaaa ctaaaagatt gcttatttaa aatctcaaaa gataaaaatg
     2401 agatattact ttcaggttca gaataataaa aaacatcctc tgctatattt gtaaaatgtt
     2461 ccagcagtag cgtaaatttt aaattactga ttttagcagc gtaactaaaa aaaacttttt
     2521 tagggtcctt tagattttca gataaatcat ttgaagtatt tgctgatatt tgtgctgtgt
     2581 tgctcataaa tctctattgt ttacttaagt aaaattaagt tattaggtta aaagttcata
     2641 agatgaaaac taaaaattca tatctttttc tacagtatcc agaattctca aataactatc
     2701 atatctttct tcagaaatat tgccattttc aactgcatct attactgcgc agccaggttc
     2761 gtgatgatga gtacaggtat taaaacgaca actggttatg aagttttcaa attctttgaa
     2821 ataatgtccc aaatcttctt tccttattcc aaacggatca atttctcgaa ctcccggtgt
     2881 atcaattata taggttttct cgccgacaaa aatcataaca cttgttgatg tagtatgggt
     2941 cccttttgat gtgtaattgc ttatctcgcc aactttaaga tttaaatcag gatagaaaag
     3001 atttagtaaa gatgatttac ctacgcccga ttggccccaa aaaagatttt tcttcccttt
     3061 taatgatttg aataaataat ccaagcctgt ggagtttatt gttgaagtta gaaacacttt
     3121 gtaaccgatc tttttataaa gatcagccca gggtttaaca gatgattcat cagcaagatc
     3181 tgttttattt attattatgc tggtttttat gtgcgcactt tcgcaggcga ctaaaaatct
     3241 atctaaagtt ttattattaa aatccggttc taaagtactg gttacaataa aaatattatc
     3301 aatgttggat gcaattatct gttcaagtct ttctccgcgg aaacttgccc cacgtgtttt
     3361 tattgctttg cgggaaagat aatttttacg cggaagtatt tcataaataa caccggttcc
     3421 atcgtcattc aaatcataaa gcacgtaatc accaacggca gcaaaatctg tattaaaaag
     3481 tttatccttt tttaaattaa aatccttttt aaattttcct tttagtgagc atctgataag
     3541 tgaatgatct gcatctgaaa agatataaaa atctttactt tccgatcttg aaatgatacc
     3601 ttgaattata ttctcctttt taaaataaaa aaagcaccat atcgaaaata tggtgcttga
     3661 attctttaag caataattaa aatatttaaa atcggaatgt agtagtaagc attacggtct
     3721 gttgtgttat atcctgatat gttcttgata catttacatc ataattatct gcaatatctt
     3781 tccaccagcc ataagcgtat gctatatcaa ttccaaaaac accttcaaaa ttaaatccaa
     3841 cacctgcagt taaatatttt ctgtcaaacg aagcgtcatc agcttcaaaa ggagattgct
     3901 gataaatacc accagctctc aattttatgg gaacaagagg taaagttaat tcggcaccca
     3961 aatttgcgtt atatgctacc ctaaggaatt ttttaatgtc cgagtttcta tcagcaaaat
     4021 atgcaggatc gattccttcc ggatttttaa attccatttg agtgtaatca attatattta
     4081 tctgcccgct gataatcaca aaagataaat taaaagccgc tcctgcagta aattcgtatg
     4141 gagtttgtat atcgtatttt actttatcac tgtacttgta tgtatccagg aaatattttt
     4201 gtcctgttcc aaattcactt gaaccattta cattaaagtt ttcctcaata gtgtatattt
     4261 taggaaactg tattgtcaat cctaatctca tcaatgattc ccactggtac atcattccta
     4321 attttccgct gtacccgcca atatcccatt gcaataagtt tttcagatta aatgttctaa
     4381 aatcttcagt ttgaggttca cccacggcgg tgataccctc ataatatttt tgtgtatcat
     4441 cttcataata atcattgttt gattcaaaac ttcctgtatg aattgtaaag cttccgccaa
     4501 taaaagtgtt ctttgcaatc tcaatagcac cggaaaagtc ccaattgtta atggctcccg
     4561 tgtttaaaat cgttccgctt tgatttaaac ttccaagaat attagtagta tcttttatgt
     4621 agttattatt ttggtcatag agtgggtaac tgagttttaa atcataagga atatcgctgt
     4681 tttgattgga aaaataattt atccaagaag tgttaccact attaaatccg ttaaattcta
     4741 aaataccgga taaatctttg ctttgattat atccaattcc tactaccata gagcctcttt
     4801 ttgtaggaaa tggaagcaca atgcttgcct gattaaattt tgtgctgttc gttttagaac
     4861 tcgattcatt acctaaaaaa ctaacatcgt tattaaaatt tgaaaaatca aagcctgctg
     4921 taagcattga acttttgatg agtgctaatc ctgcaggatt aaaaaatgta ccggcagcat
     4981 tgtcaccaac agccacaaaa gcatttccca ttcctaaagc ccgggcatca ggacctaaac
     5041 caggaaagcc tactcttata gcatcattat aattttgggc tataccggat atggttaaaa
     5101 aattagcaat gaaaaaaatc cgtacaataa ttttagtaaa cattttattt atctcctgcc
     5161 gtttcctgaa tttcgtgtat cagaagaacc tctgttatta ttgttgctag tacttgaaga
     5221 attattgttt gaactcgaat atgattcact tcttgttaca ttattgcttg taaaatcagt
     5281 atctctgccg gaactacctc tgccgtttcc atcatttctt atattgttat tcccattacc
     5341 attatcgtta gttctttctt tttgcggcgg attagttcct ttaaccggtt gatcatagcc
     5401 atttgatggc ggatagtatg taggtggagg cggaacagga ataggttcat agattatcac
     5461 tggtgcatac tctgttgtcg taacatatcc tgatgaatca ctttcaggta aatcaagaat
     5521 cgtgtaacaa ccctgtaaaa gtgtcatgac agaaaaaaca gcgattatag atattaattt
     5581 tttcatttta tttaccttct tccggaatta ttgcttccgc tatttcttgg aggatttgaa
     5641 tttgtgtttc ctctgggtgg tgaagaagtt ctgccactag gaggactata actccttggt
     5701 gtattattgt tcgattttgg aggattataa ttcctatttg gtgatttatt agtatttgtc
     5761 tcaggtctct tatttacatt tgttttatta tccctgatag tgtttggatt tttcttatcc
     5821 gtactttttc tattaggatc agtaactctt gtattattgg tattctttct tgtatcagta
     5881 cgattaataa catcattcat tcgggaatcc ttaacagtat tattttttct cattgtttcg
     5941 gttgttcgtg tatttatcgc atctaatgtt ctggtagtag tctctctgct ttttgtatct
     6001 cttacactag ttcttaatcc agtcaattca cgatttttat catcacgccc ggatacaatt
     6061 tgtttctctc ttgttttaag ttgatctctc aatgttgaag ttccgtcggt ggaagtatat
     6121 aaatttctat ctcctccgct gtttctgcct ccggaattat tcctgatctt atatccatca
     6181 ttcgttctgt atttataagt atcatagtaa ccattgtcat agtatggata tgaatcataa
     6241 tatccataac aataatttgg ataccagtaa gttggataat agtaataa
//
LOCUS       DASWBY010000202         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_82379, whole genome shotgun sequence.
ACCESSION   DASWBY010000202 DASWBY010000000
VERSION     DASWBY010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82379"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VF870_03670"
     CDS             <1..399
                     /locus_tag="VF870_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9251312.1"
                     /translation="SAGASRSFMAFLIDAYYEEEVNGEVRSVLRFHPRLAPIKAAIFP
                     LVNKDGMPEISREIEKDLRRNFKVFYDDKGAVGRRYRRQDEAGTPFCITVDTQTIEDK
                     TVTVRDRDSMVQERVAIDHLTRYLSDKLLS"
     gene            515..1657
                     /locus_tag="VF870_03675"
     CDS             515..1657
                     /locus_tag="VF870_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9251313.1"
                     /translation="MKKVFLSILLFNISIFAQNSNFDSRVNEGIKQIYNIKFTEAEKT
                     FRSVIADYPNQPAGRFFLAMIDWWKILLDPDNESYDEIFFQKLEDVIYQCDQLLDKNP
                     KNVDALFFKGGSIGFRGRLRAFRESWLKAADDGREALPIVEEAAALDPNNLDVQLGFG
                     IYNYYADVIPSEYPMVKPLMIFFPDGDKAKGIQQLKNTAFNGKFAKYEARYFLMTLYY
                     RYENNAIAADEFSKMLCDDFPDNPVFEKWRGRIAVKRGDYFLADSVFKDVLAKADKKY
                     FGYNTPNSLREAAYYVGNNYKNNGQIDSAKIYLERCISESKKIDEEGEESGFQVNAVL
                     FLAQMAETQNNKDEAIRLYEKLLDMNDYGRSHSIAESSLKSLKGTK"
     gene            complement(1735..>2360)
                     /locus_tag="VF870_03680"
     CDS             complement(1735..>2360)
                     /locus_tag="VF870_03680"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9251314.1"
                     /translation="YGSFEGADDFGTKTSEFSAGEFAFVLGYANQLDDNFYYGANAKF
                     IYSSIETSYSTAMAVDLGLHYAIPDKNWNFGFAVLNLGGQISKYYNTTEDLPLDVVIG
                     LSKRLENLPLRLSLDFHKLNEDRDEFIERFKAFTVGAEFTLSKVLKLRLGYDNERRSE
                     FKIGTTAGVAGFNAGLGVKVSDYQFDYGYSSLGLAGGLHRIGISTSL"
BASE COUNT          813 a          390 c          437 g          720 t
ORIGIN      
        1 tctgccggag ccagccgcag ttttatggca tttttaattg atgcttatta tgaagaagaa
       61 gtaaatggtg aagtgagatc agttttaaga tttcatccaa gacttgcccc aattaaagca
      121 gcaattttcc cattggttaa taaagacgga atgcctgaaa tttcccgcga gattgaaaag
      181 gatttacgtc gcaactttaa agttttttat gatgataaag gtgcagttgg cagaaggtac
      241 agaagacagg atgaagccgg aactccattt tgcataactg tagatacgca aacaattgaa
      301 gataaaactg ttacagttcg tgatagagat tcgatggttc aggaaagagt tgcaattgat
      361 catttaacta gatatttgag tgataaactt cttagttaat taagaattaa aaattaacaa
      421 ataacaatta ggcgatttta ataatcgcct ttttgtttgc acttgtttat atcgtcaatc
      481 atttaaaaat tgtactgtaa ttttttaagg caatatgaaa aaagtatttc tatccattct
      541 attgtttaat atttcgattt ttgctcaaaa ttctaatttt gattcacgcg ttaatgaagg
      601 aatcaaacaa atctacaaca tcaagtttac cgaagcagaa aaaacatttc gttctgtgat
      661 agcagattat ccaaatcaac ctgcaggtag attttttctt gcaatgattg actggtggaa
      721 aattcttctt gatccggata acgaatccta tgatgaaata ttttttcaaa aactggaaga
      781 tgtaatttat caatgtgatc aattgcttga taaaaatcct aaaaatgttg atgctctttt
      841 ctttaaagga ggatcaattg gattccgcgg cagacttcgt gcattccgtg aaagctggct
      901 aaaagccgct gatgatggac gtgaagcatt accaattgta gaagaagcag ccgcacttga
      961 tccaaataat ttggatgtgc agcttggttt tgggatttat aattattatg cagatgtaat
     1021 tccgagcgaa tatccaatgg taaagccatt aatgattttc ttccctgacg gtgataaggc
     1081 aaaagggatt cagcaattaa agaatacagc ttttaatgga aaatttgcaa agtatgaagc
     1141 tcgatatttt ctgatgacac tttattatcg ttatgaaaac aatgcaattg ccgctgatga
     1201 attctcaaaa atgttatgtg atgattttcc tgataatcct gtattcgaaa aatggcgcgg
     1261 aaggatagca gtaaaaagag gagattattt tttagccgat tcagtcttta aagatgtttt
     1321 agcaaaagca gataaaaaat attttggcta caacacgcca aactcacttc gggaagccgc
     1381 atactatgtc ggcaacaatt ataaaaacaa cggacagatt gattcagcaa aaatttattt
     1441 ggaaagatgt attagtgaat caaaaaagat tgatgaagaa ggtgaggaat caggttttca
     1501 ggtaaatgca gttttatttc tcgcacagat ggcagaaacc cagaataata aagatgaagc
     1561 aatcaggttg tacgaaaagt tacttgatat gaatgattat ggaagatcac attcgatcgc
     1621 ggaaagtagt ttaaaaagtt tgaaaggaac taaatagtca ttgcgaacga gtcttcgagt
     1681 gaagcaatct catatgctta aaatgcgaga ctgcttcgtt cctcgcagtg acatttaaag
     1741 agaagtggaa ataccaatcc tatgcaggcc accggctaat ccaagagaag agtaaccata
     1801 atcaaattga taatccgaaa cctttacacc aagtccggca ttaaatccag caacgccggc
     1861 agtcgttcca atcttaaact ctgagcggcg ttcgttatca taacctaatc ttaattttaa
     1921 gactttgctt aatgtaaatt cagcgccaac agtaaatgct ttaaatcttt caataaattc
     1981 atcacggtct tcatttagtt tatgaaaatc taatgataaa cgtaatggaa gattttctaa
     2041 tctttttgaa agtccaatta caacatccaa gggcaaatcc tctgttgtgt tgtaatattt
     2101 tgaaatttgt ccgccaaggt ttaacactgc aaaaccaaaa ttccaattct tatcaggtat
     2161 tgcatagtgc aaacctaaat caactgccat cgctgtggag taactggttt ctatactgga
     2221 ataaataaac ttagcattag caccataata aaaattatca tcaagctgat tggcataacc
     2281 gagtacaaaa gcaaattcac ctgcactaaa ttcacttgtt tttgttccaa agtcatctgc
     2341 accctcaaaa ctgccgtagt
//
LOCUS       DASWBY010000203        10877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_122976, whole genome shotgun sequence.
ACCESSION   DASWBY010000203 DASWBY010000000
VERSION     DASWBY010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10877
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122976"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..405)
                     /locus_tag="VF870_03685"
     CDS             complement(172..405)
                     /locus_tag="VF870_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9251315.1"
                     /translation="MDVEAKVKEIIIDKLGVEESQITPEASFTNDLGADSLDIVELVM
                     GFESAFQISIPDEDAEKIGTVGDAVKYLSEKLA"
     gene            complement(503..1279)
                     /gene="fabG"
                     /locus_tag="VF870_03690"
     CDS             complement(503..1279)
                     /gene="fabG"
                     /locus_tag="VF870_03690"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX9251316.1"
                     /translation="MTSKVVESHNKRAIVTGGTRGIGRAIVKELASRSCCGVLFSDVA
                     FVYNSCDECAEELLNEIDYPGIKIHGFKADATSMEQAQATVDAAIEKLGGVDILINNA
                     GITRDNLLLRMTEKDFDDVISANLKSVFNYTKAVIKPMIAQRYGRIVNIASVVGLIGN
                     AGQSNYSASKAGVIGFTKSMARELASRNITVNAVAPGFIQTDMTNKLTEEQVKKLVQN
                     VPMSRLGNPEDVAKVVAFLCSKDADYITGQVIAVDGGMTM"
     gene            complement(1269..1877)
                     /locus_tag="VF870_03695"
     CDS             complement(1269..1877)
                     /locus_tag="VF870_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HEX9251317.1"
                     /translation="METSSPQKFTKSINGVYIDPQIFTFKFSCRCGGECCNYGVYTDL
                     KEHEYILSIKDKIIPLLDDSQSKEVDEWFEEPEEDEDFESGVAVGTEIINGKCTFLDK
                     NGLCTLQKLAMIKGEHKWKYKPIYCVLFPLTIFEGALTIDDEHIDRLKSCSKDPIKET
                     SIYDACKEELIHFFGPEKFLELENYKNEYLSEYYSGVEKNDK"
     gene            complement(1877..2791)
                     /gene="fabD"
                     /locus_tag="VF870_03700"
     CDS             complement(1877..2791)
                     /gene="fabD"
                     /locus_tag="VF870_03700"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX9251318.1"
                     /translation="MSKKAFLFPGQGSQYIGMAKDLFDKSVEAKEMIKTADDALGVNL
                     SYIMFNGPEDQLKQTEYTQPAIFLHSVVLASLIRTLHPEASAGHSLGEYSALVSSNAI
                     QFYDAIKLVRTRGKAMQQAGIDNPGTLAAIVGLEPAKVEELCKEASSNGIVQCANFNS
                     PGQIVISGSIDGVRKAMELCKANGAKLTKELVVSGAFHSPLMQSAKDMLLDALALTPV
                     YDARIPVYANVTAKPVTIKDEIKNLLHEQVTSPVRWEETIKNMITDGFDEFYEIGPGK
                     VLQGLVKRINPDVKLFGIDKYEDLEHYL"
     gene            complement(2815..3813)
                     /locus_tag="VF870_03705"
     CDS             complement(2815..3813)
                     /locus_tag="VF870_03705"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX9251319.1"
                     /translation="MSEKRYNAIITGVGMYTPDKVLDNKYFESIVETNDEWIKTRTGI
                     SERRILENGATSDLAARAALDLLEKHNVNPEEIDAIIVATVTPDMFFPATACLVQNKI
                     GAANAWGFDVSAACSGFLFALQTGASLIESGRYKKVLVIGADKMSSIVDYTDRNICIL
                     FGDAGAAVLLEPTEDKTLGVQDSILRVDGTGKDALYMLGGGSLNPASHETVDKKFHYI
                     YQDGKAVFKVAVKGMADVSAELMEKSNLKSDDVAYLVPHQANLRIIDATAQRMGISKD
                     KVMINIDKYGNTTAATIPLCLVEYYRAGKVKKGDNLILSAFGAGYTWGATHVVWAI"
     gene            complement(3828..4859)
                     /gene="plsX"
                     /locus_tag="VF870_03710"
     CDS             complement(3828..4859)
                     /gene="plsX"
                     /locus_tag="VF870_03710"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX9251320.1"
                     /translation="MDESEAKNSKCRIIVDAMGGDYAPQNAVTGAIDAYNEKKDFELF
                     LIGKKDEILSVIKSGQLSFDEKYIIHADEVIEMGDSPTSALKAKPDSSIVRGAQLVKE
                     GKADAFVSAGNTGAMMSASTLIMGRIPGVGRPTIGAEMPNVHGICYFYDVGAGKDAKP
                     SHLFEYAVMGTIYAKEMGGIKNPTVGVLSMGEEEGKGNEVSNEAFKLLKDSKLNFIGN
                     IEGRDIMTKQVDVIVCDGFVGNIILKFGESVPKLLKHLLTKTAEDSFLDKLKVGISKG
                     TLKKALKSLDYQEHGGVPLLGVNGISIIGHGSSSPKAIKNMVLKAREMYNKDLIKKIE
                     TSIKHYSQK"
     gene            complement(4881..5060)
                     /gene="rpmF"
                     /locus_tag="VF870_03715"
     CDS             complement(4881..5060)
                     /gene="rpmF"
                     /locus_tag="VF870_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX9251321.1"
                     /translation="MPNPKRKMSKSRRDKRRTHYKAVAPSLSTCGNCGEMKLAHRACP
                     SCGYYNERSVFIPKS"
     gene            complement(5079..5585)
                     /locus_tag="VF870_03720"
     CDS             complement(5079..5585)
                     /locus_tag="VF870_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX9251322.1"
                     /translation="MIIKISNLSEGEHNFVFEDKVDVLDLDKPFYGKYKSSVLLDKLH
                     DQLILSVTSDFKVKYECDRCGTEFKTSLKSDYKMIYLMNEAPEETDSINVSYLSRDAD
                     KIDITSDVREFALLAIPMKKLCKEDCKGLCYKCGKDLNKERCTCSTEEIDPRWKPLMN
                     LKDKLNLN"
     gene            5905..6849
                     /locus_tag="VF870_03725"
     CDS             5905..6849
                     /locus_tag="VF870_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEX9251323.1"
                     /translation="MLIIFSALAAIVPMSIYLVLIWKFDRYDREPFKLVLTNYLWGAF
                     GAIVLALLGSLFLTTIASFFIRDNLQLSRFGAIVVAPLVEEFTKGLFLLITIVNKKFD
                     NITDGIVYGGAIGLGFGMTENFLYFVTYGESISNWIMLVIVRSLFSAVMHCVSTATLG
                     AFLGSAKFKSSSKKVLYALIGFFIAAMIHSIWNFSLSYEKIAPIGFLFMFISIIIFIS
                     VFSISLHGERRIIFNELKEESDNGVIPESHLVILSSPQRERKGWLDERTRIPYIKAAT
                     TLAFRKVQLRNSSGASKAYYELEVENYRDFIKKLLQNT"
     gene            6853..7548
                     /locus_tag="VF870_03730"
     CDS             6853..7548
                     /locus_tag="VF870_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251324.1"
                     /translation="MNSKAVYIFPFFASVSIELYDSFSGFSKPDVVMLSISLYLNILE
                     NLNGKEDRIDIYCLWDEKDRDYLPVELKNDNLKIIFTDISNKKIIFEKLSNKEFSRYK
                     NNLIVFSDVIDIKPSDYEQYFNLLNIEDESLVIAKSKENNIAVFGFNNYSNEIINSLL
                     LSGFDYNNFLSRIKSCAHFMHIVNDLLVVRSLINFKQLYVALSQKKSIEYCSQQMHER
                     FTHLFVEYKDLLK"
     gene            7545..8126
                     /gene="nadD"
                     /locus_tag="VF870_03735"
     CDS             7545..8126
                     /gene="nadD"
                     /locus_tag="VF870_03735"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX9251325.1"
                     /translation="MSKVGIFGGTFDPIHNGHLITAQSVKEIRNLEKIIFIPAYISPH
                     KQHAKASSAQHRLNMLKLAVDDIPFFECSDFEINQHTISYTIDTLREFKKSYDEIDLI
                     IGYDNIFQFHTWKEPDEIFRMANVVVLKRKSSHPLDFVDKYVEAAHFVETRGIEISAT
                     DIRNRVHADLPIYYLVPEKVLEYISKNKLYKDS"
     gene            8127..9050
                     /locus_tag="VF870_03740"
     CDS             8127..9050
                     /locus_tag="VF870_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9251326.1"
                     /translation="MKILVTGGAGFIASQIADAFIQEGHQVVILDDLSTGFEKNVNPK
                     AKFIKANICDKSLDKLFETEKFDVVNHHAAQMDVRRSVKDPEFDATTNILGTINLLQN
                     CVKYGVKKFMFASTGGAVYGEQDYFPADEKHNQQPKSPYGISKLAVEKYLYFYNSEHK
                     LNYTILRYANIYGPRQNPFGEAGVVAIFSTKLLKGDQPVINGHGKQTRDYVFVGDVVK
                     ANLIALRDEASDVYNIGTGIETDVNQLFNYINDITKANKEEKHGPTAAGEQLRSVITS
                     DKLFKKFGWRPSTKLEEGLKTTVDFFKNNLT"
     gene            9052..10005
                     /gene="meaB"
                     /locus_tag="VF870_03745"
     CDS             9052..10005
                     /gene="meaB"
                     /locus_tag="VF870_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9251327.1"
                     /translation="MLDLNLIENIYKEDRRSIARVISIVESNNTAASEYLKEIYKKVG
                     SAYRIGITGPPGAGKSTITNQLTKYYRNQNKKVGIIAVDPTSPYTGGALLGDRVRMTD
                     IGMDQGVFIRSMATRGSLGGLSKKAIDAADVLDAAGFDIILLETVGVGQSELDIAQAA
                     DTTIVVLVPESGDSVQAMKAGLMEIADFFVLNKSDRPGSQQAYTALQTILMIKDHDEN
                     SWLPNIIKAIASENKGIDEISTEIERHKDFMKNKNLFHQKREKQAKVRIKEIVEHKLK
                     DELWSETGENSLNSSLQKVILGNLSPYHIAEEIIEEFKNRI"
     gene            10090..>10877
                     /locus_tag="VF870_03750"
     CDS             10090..>10877
                     /locus_tag="VF870_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9251328.1"
                     /translation="MEQIPFLSGLTKNQIIIRDTIKDFAEKNIRPVIMEYDESQKFPM
                     EIMKQLGELGFLGILVPEEFGGAELGYVEYAIIIEEISKVDPSIGLSVAAHNGLCTNH
                     INLFGNESQKKKYLPDLASGRKIGAWGLTESASGSDAAGLLSTAVKNGDHYILNGSKQ
                     FTTHGTVGETYVVMAITNKEAGKKGISAFILEKGMEGLIIGKKENKLGMRASDTTQLA
                     FENCKVPAENLLGEEGMGFINSMKVLEGGRISIAACSIGLAQGC"
BASE COUNT         3539 a         1947 c         1763 g         3628 t
ORIGIN      
        1 ttaagccttg ccaaaattga ggaacatcta agccaattgg agtgatagca ccgataccgg
       61 ttattacaac tcttcttcta gccataaaac ctcatcagca taagccattt ttgagatgga
      121 aagacatata aaggtaagat agtcgggatt aaaaaatccc gaccaattta attaagctaa
      181 tttttctgaa agatacttaa cagcatctcc aactgttccg attttctcgg catcttcatc
      241 aggaattgaa atctgaaaag cactttcaaa tcccataact aattctacga tatcaagtga
      301 atcagcgccc aggtcattag taaatgatgc ttcaggagtg atttgagatt cttcaacacc
      361 taatttatca atgatgatct ctttcacttt tgcttcaaca tccatggtat tctcctatta
      421 ttggttttgt atttatttga gttattattt attggtctaa atttaacaaa attaaaatct
      481 aagaaacaac tagaattatt aattacattg tcattccgcc atctacggca ataacctgac
      541 ctgtaatata atctgcatct ttgctgcata aaaacgcaac aactttagca acatcttctg
      601 ggttgcctaa tcttgacatt ggaacatttt gcaccaactt cttaacctgt tcctcagtta
      661 atttatttgt catatctgtt tggataaatc ctggtgctac agcattaaca gttatattac
      721 gggaagcaag ttcacgagcc atcgattttg taaatccaat aactccagct tttgaagcgg
      781 agtaattaga ttgtcctgca tttccaatca atccaacaac agaagcgatg tttacgattc
      841 ttccatatcg ctgagctatc attggtttta ttactgcttt agtataatta aacacactct
      901 ttagatttgc acttattaca tcatcaaaat ctttttcagt cattcttaat aataaattgt
      961 ctcttgtaat acctgcatta tttataagga tatcaactcc gccgagcttt tcaattgctg
     1021 catcaaccgt tgcctgagcc tgttccatag acgttgcatc agctttaaat ccatgtattt
     1081 taattccggg ataatcgatt tcgttcaaca actcttcggc acattcatca cagctattat
     1141 aaacaaatgc aacatctgaa aataagactc cgcagcagct tcttgaagca agctctttaa
     1201 caatcgctct gcctatacct cttgttccac ctgttacaat ggctcgttta ttatgtgatt
     1261 ctacaacttt acttgtcatt tttttcaact ccggaataat attcggataa atattcattt
     1321 ttatagtttt caagttcgag aaacttttcc ggtccaaaga aatgaattag ttcctctttg
     1381 catgcatcat aaattgatgt ctctttgatc ggatctttac tacaagactt taacctgtcg
     1441 atatgttcgt catctatggt taaagcgcct tcaaaaattg tgagcggaaa caaaacgcaa
     1501 taaattggtt tatatttcca cttgtgttca cctttaatca ttgcaagttt ttgaagagta
     1561 cataatccat ttttatctaa aaaagtacat ttgccattta taatctcagt accaacggca
     1621 acacctgatt caaaatcctc atcttcttca ggctcttcaa accattcatc aacttcttta
     1681 gactgggaat catcaagtaa cggtataatc ttatctttaa tacttaaaat gtattcgtgc
     1741 tctttaagat ccgtataaac tccatagttg cagcattcgc cgccacatct gcaagagaac
     1801 ttaaaagtaa atatctgagg atcaatataa acaccattta tcgatttagt aaatttttga
     1861 ggtgaactag tttccattat aaataatgct ccaaatcttc gtatttatct attccaaata
     1921 atttaacatc aggatttatt cttttaacta atccttgcaa aactttacca ggaccaattt
     1981 cataaaattc atcaaagcca tcagttatca tatttttgat tgtttcttcc catctaactg
     2041 gagaagttac ctgctcgtgc agtaaattct ttatttcatc ttttatggta actggttttg
     2101 ccgttacgtt tgcataaact ggaattcttg catcgtacac tggcgtaaga gccaatgcat
     2161 caagtaacat gtcttttgct gattgcatca gcggtgagtg aaatgctcca cttacaacaa
     2221 gttctttagt taacttggca ccattagctt tacataattc cattgctttt ctaacaccat
     2281 caattgatcc tgaaattaca atttgtcctg gtgaattaaa atttgcacat tgaactatac
     2341 catttgaaga agcttcttta catagttctt ccactttagc cggctctaaa ccaacaatgg
     2401 ctgcaagtgt cccaggatta tcaatccctg cttgctgcat tgctttgcct cttgttctaa
     2461 ctaattttat tgcatcataa aactgaattg catttgaaga aaccaatgca gaatattcac
     2521 caagcgagtg ccctgcggaa gcttcagggt gaagtgttct tattaaactt gcaagaacaa
     2581 cgctatgcag aaatatagcg ggttgcgtgt actctgtttg ttttaattga tcttcaggtc
     2641 cgttaaacat tatgtatgaa agattaacac caagtgcatc atcagccgtt ttaatcattt
     2701 ctttggcttc aacagattta tcaaataaat ccttagccat accaatatat tgcgatccct
     2761 gacctggaaa taaaaatgct ttttttgaca ttaaactagt ttaagtttta ataattaaat
     2821 tgcccacact acgtgagtag ctccccaggt atatcccgca ccaaaagcag ataaaatcaa
     2881 attatcacct ttttttactt ttccagcgcg atagtattca actagacata atggaattgt
     2941 tgctgctgta gtattgccat atttatcaat attaatcatc actttatctt tactaattcc
     3001 cattctctgc gctgttgcat caataatcct taaatttgcc tgatgaggaa caagataagc
     3061 aacatcatcc gattttaggt tacttttttc cattaattct gcagatacat cggccatacc
     3121 tttaacagca actttaaaaa ctgctttgcc atcctgataa atgtaatgaa attttttatc
     3181 aactgtttcg tgtgaagccg gatttagact accgcctcca agcatataca aagcatcttt
     3241 acctgttcca tcaactctta aaatggaatc ctgaacaccc aaagttttat cttcagtcgg
     3301 ttccaataat acagcagcac ctgcatcgcc aaaaaggata caaatatttc tatcagtata
     3361 atcaacgatt gaactcattt tatctgcccc gataactaat acttttttat atctgcctga
     3421 ttcaataagt gatgctcctg tttgcaatgc gaacagaaat cctgaacaag cggcagagac
     3481 atcaaatccc caggcgttag cagcgcctat tttattttgt accaaacaag ctgttgccgg
     3541 aaaaaacata tccggagtaa cagtcgctac aataattgca tcaatttctt ccggattaac
     3601 gttatgtttt tctaacaaat ccaatgctgc ccgagcagct aaatcgcttg ttgcaccatt
     3661 ttctaaaatt cttctttcac taattccggt tctggtttta atccattcat cgttagtttc
     3721 tacaattgat tcaaaatact tattatctaa aactttatca ggagtataca ttccaactcc
     3781 tgttataata gcattatatc ttttttctga catcaatatt ccattattta tttttgtgaa
     3841 taatgtttga tagaagtttc tatcttttta ataagatctt tattatacat ctcgcgtgct
     3901 tttaacacca tattcttaat tgcttttggc gagctggatc catgcccaat aatacttatt
     3961 ccattaacgc ctaaaagtgg tactccgccg tgctcttgat aatctaagga tttcaaggct
     4021 ttttttaatg tgccttttga aattccaact tttaatttat ccagaaaact atcttcagct
     4081 gttttagtta aaagatgctt gagaagtttg ggaacagatt cgccaaattt tagaataatg
     4141 tttccaacaa atccatcaca gacaataaca tcaacctgtt ttgtcattat atctctgcct
     4201 tcaatatttc cgataaaatt cagcttggaa tctttaagca atttaaatgc ttcattcgaa
     4261 acttcatttc ctttgccctc ttcttcaccc atagataaaa ctccaactgt gggattttta
     4321 attccgccca tttcttttgc atagattgtt cccatcacag catactcaaa aaggtgcgaa
     4381 ggttttgcat ccttacctgc gccaacatca taaaaataac aaataccatg aacatttggc
     4441 atttcagctc cgattgtcgg gcgaccaaca ccaggaattc ttcccataat taatgttgat
     4501 gcactcatca ttgccccagt atttcctgca cttacaaatg catctgcttt accttcttta
     4561 actaattgtg cgcctcttac tattgaagaa tctggtttag cttttaatgc agaggtgggt
     4621 gaatcaccca tttcaataac ttcatcagca tggataatat acttttcatc aaaagagagc
     4681 tggcctgatt taattacaga cagtatctca tcctttttcc caatcagaaa aagttcgaaa
     4741 tctttctttt cattgtaagc atcaatagct ccagtaacag cattttgagg ggcatagtcg
     4801 ccccccattg catcaacaat aattctgcat ttagaatttt tagcttctga ttcatccatt
     4861 agataagtga gctataaaaa ttagcttttg ggaataaaca ctgagcgttc attgtagtaa
     4921 ccgcaggaag ggcaagcacg atgagcaagt ttcatttcgc cacaattacc acaagtgctt
     4981 aatgaaggtg ctactgcttt atagtgagtt ctgcgtttat ctctacggct cttggacatc
     5041 ttacgtttcg gatttggcat tatatcattc cattatattt aattgagatt taatttatct
     5101 ttcaagttca taagaggttt ccacctcgga tcaatttctt ccgtgctgca agtgcatctt
     5161 tctttattca aatctttgcc gcatttataa cacaatcctt tgcaatcttc tttacaaagt
     5221 tttttcatag ggattgcaag caaagcgaat tcacgaacat ctgaagttat atcaatctta
     5281 tcagcatccc tgctgagata acttacatta atagagtccg tttcttctgg tgcttcattc
     5341 ataagataaa tcattttata atcgctcttt aaactggttt taaattcagt tccacatcgg
     5401 tcgcactcat attttacttt aaaatctgag gttaccgaaa gtattaactg atcatgcagt
     5461 ttatcaagta atacagacga tttatatttt ccataaaatg gtttatctaa atccaataca
     5521 tcaactttgt cttcaaaaac aaaattgtgt tccccttcgc ttaaattaga tatttttata
     5581 atcatcttgg aaaatcacgc aaaaaacgag gctaaatata taactgaacc actatataat
     5641 caaacaatgt taaatctcta aatatttggc tttgggaaga attacgccat tttttaacat
     5701 taccggatat tattgaagcc aaatataaca caatttagtc acagggtaat gatttgataa
     5761 gaaaagtacc agcgtacaga tgcttttact gtttaacata aaagtttatt tagaaatttg
     5821 ttccaaaata attttggttt tttcactcgt aaatcaataa tttgaagcag atatttaata
     5881 atagttatca ttgccaaaaa ttaaatgtta ataatttttt cagctttagc tgcaatcgtt
     5941 ccaatgtcaa tctatttagt gttgatatgg aaatttgatc gatacgacag ggaaccattt
     6001 aaacttgttt taaccaatta tctttggggt gcatttggtg ctatagttct ggctttactt
     6061 ggcagtttat ttcttaccac aatagcttca ttttttatca gagataattt acagctaagc
     6121 aggttcggag ctattgttgt cgctccattg gtggaagaat ttacgaaagg tcttttttta
     6181 ctgattacga ttgtcaataa aaaatttgat aatataaccg atggtatagt ttacggcggc
     6241 gctattggcc taggatttgg aatgaccgaa aactttcttt acttcgttac ttatggagaa
     6301 tcaatatcaa attggatcat gctggtaatt gttagaagcc ttttctctgc cgttatgcac
     6361 tgtgtttcaa ctgcaactct tggcgcattt cttggatcag caaaatttaa atcatcttca
     6421 aaaaaagttc tttatgcact aattggattt tttatcgcgg ccatgattca ttcaatatgg
     6481 aatttcagtc ttagttatga aaagatagca ccaatcggtt ttctttttat gtttatttct
     6541 ataattattt tcatttctgt tttttctatt tcattacatg gtgaaagaag aataatattt
     6601 aatgagctta aagaagaatc cgataacgga gtgattccgg aatctcacct ggttatttta
     6661 agttctccac agcgtgagcg taaaggctgg cttgatgagc gtacaagaat accttatatc
     6721 aaagcggcta caacacttgc atttagaaaa gttcaattaa gaaactccag tggtgcaagc
     6781 aaagcttatt atgaattgga agtagaaaac tatagagatt ttattaaaaa attattacaa
     6841 aacacttaac agatgaattc aaaagctgta tatatttttc cattcttcgc ttctgtttca
     6901 atagagttat atgatagctt tagcggattt agtaaaccag atgtggtgat gctaagtata
     6961 tctctttatt taaatattct ggaaaatctt aacggcaaag aggatagaat agatatttac
     7021 tgtttatggg atgaaaaaga tagagactat cttcccgttg agttaaaaaa tgataatctt
     7081 aaaataattt ttacagatat ttcaaataag aaaataattt ttgaaaaatt atcaaataaa
     7141 gaattctcac gatataaaaa taatctgatt gttttttctg atgtaattga tataaagcca
     7201 tctgactatg aacaatattt taatttgctt aacatagagg acgaatcact cgtaatagca
     7261 aaaagcaagg aaaataatat tgccgttttt ggatttaata attattcgaa tgaaataatt
     7321 aatagtttat tacttagtgg atttgattac aataattttt taagtcgtat caaatcctgt
     7381 gcgcacttta tgcacatcgt aaatgattta ttggttgtaa gaagtctaat aaacttcaaa
     7441 caactctatg tggcgttatc acaaaagaaa agtatagaat attgttccca gcaaatgcac
     7501 gaacgattta cacatctttt tgttgaatac aaggatttac ttaaatgagt aaagttggaa
     7561 ttttcggcgg tacatttgat ccaatacata atggacactt aataaccgca caatcagtca
     7621 aagaaattag aaatcttgaa aagataattt ttatacctgc atacatatct ccacataaac
     7681 aacacgcaaa agcttcaagc gcacagcatc gattaaatat gctaaaactt gctgttgatg
     7741 acatcccttt ttttgagtgc tcagattttg agataaatca gcatactatt tcttatacaa
     7801 ttgatactct tagagaattt aaaaagtctt atgatgaaat tgatttaata ataggctacg
     7861 ataatatttt ccagttccac acctggaaag aacccgatga aatatttaga atggcaaacg
     7921 ttgtagtgct taaaagaaaa tcttctcatc cattagattt tgttgataaa tatgttgagg
     7981 ctgctcattt tgtggaaaca agaggcattg agattagtgc aacggatatt cgcaatcgtg
     8041 ttcatgctga tttacctatt tattacctcg taccagaaaa agttttagaa tatattagta
     8101 aaaataaatt atataaggat agttaattga aaatattagt taccggcgga gccggtttta
     8161 ttgcttcaca aattgctgat gcatttattc aagaaggaca tcaggttgta atacttgatg
     8221 atctttccac cggttttgaa aagaatgtaa atccaaaagc aaaattcatt aaagcaaata
     8281 tttgcgataa gagcttagat aaactttttg aaactgaaaa atttgatgtg gttaatcacc
     8341 atgccgctca aatggatgtt agaaggtcag tcaaagatcc tgaatttgat gctacaacaa
     8401 atatacttgg gacaataaat ttattacaaa attgtgttaa gtacggcgtt aaaaaattta
     8461 tgtttgcttc aacaggcggt gcggtttatg gtgagcagga ttattttcct gcagatgaaa
     8521 aacataatca gcagccaaaa tccccttacg gtatttctaa acttgcagta gaaaaatatc
     8581 tttacttcta taattcagag cacaaactga attacacgat tttacgatat gccaatattt
     8641 acgggccaag acaaaatcca tttggcgagg ctggagttgt tgcaatcttt tctacaaaac
     8701 tccttaaagg agatcagcct gttattaatg gtcacggtaa acaaacccgc gattacgttt
     8761 ttgttggtga tgtagttaaa gcaaatctaa tagcgttaag agatgaagca tcagatgtct
     8821 ataatattgg aacaggtatc gaaacagatg taaatcaact tttcaattac attaatgata
     8881 ttactaaagc caacaaagaa gaaaagcatg gcccaaccgc tgccggtgaa caattaagaa
     8941 gtgtaataac ttctgataaa ttattcaaaa aatttggatg gagaccatcg acaaaattgg
     9001 aagaaggatt aaaaacaaca gtggattttt ttaaaaataa tttaacctag tatgcttgat
     9061 ctgaatctta ttgaaaatat ttacaaggaa gacagacgct caattgcccg tgttatttct
     9121 attgttgagt caaataatac tgctgcatca gaatatttaa aagagattta caaaaaagtc
     9181 ggcagtgctt atcgcattgg aattacaggt ccgcccgggg ctggtaaaag cacaattaca
     9241 aatcaattaa caaagtacta tagaaatcaa aataagaaag tcggaatcat agcagtcgat
     9301 ccgacttctc catatacagg cggcgctctg cttggggaca gagtaaggat gacagacatt
     9361 ggaatggatc aaggtgtttt tattcgaagt atggctaccc gcggcagtct tggcggtctt
     9421 tctaaaaaag caatcgatgc tgctgatgtt ttggatgcag caggatttga cattatactt
     9481 ttagagactg ttggtgttgg tcaatctgag ctggatattg cgcaagctgc agatacaaca
     9541 attgtggtgc ttgttccgga aagtggagat tcagttcaag ctatgaaagc tggattgatg
     9601 gaaatagcag atttttttgt tttgaataag agcgataggc caggctctca acaagcatac
     9661 acagcacttc aaacaatttt aatgataaaa gatcatgatg aaaattcctg gctgcctaat
     9721 attataaaag ctattgcatc ggaaaacaaa ggtattgatg aaatatctac tgaaattgaa
     9781 cgtcataaag attttatgaa aaacaaaaat ctttttcatc aaaaaagaga gaagcaggct
     9841 aaggttagaa ttaaagaaat tgtagaacat aaattaaaag atgaattatg gagtgagact
     9901 ggtgaaaatt ctttaaattc atctttacag aaagttattc ttggcaattt atcaccttat
     9961 cacattgctg aagaaataat tgaagaattt aaaaacagaa tttaattttt taatatcacc
    10021 ctgaatttgt ttttttaaga taaatcaatt ataaacgatt gtatttagaa accattttaa
    10081 ggaatgtcga tggaacaaat accttttctt agcggactta caaaaaacca aatcatcatt
    10141 cgtgatacga taaaagattt tgccgagaaa aatattcgtc ctgttattat ggaatatgac
    10201 gaatcacaga aattcccgat ggaaataatg aagcagctag gcgagcttgg ttttcttggc
    10261 atactcgtcc ccgaagaatt tggaggcgct gaattaggtt atgttgagta cgcaatcata
    10321 attgaagaaa tctctaaagt cgatccttcg attggacttt ctgttgcagc acataatgga
    10381 ttatgtacga atcatataaa tctttttggt aatgaatccc aaaagaaaaa gtatttacct
    10441 gatcttgcca gcggaaggaa aattggtgcc tggggtttaa cagaatccgc ttccggaagt
    10501 gatgctgccg gattattaag tactgcagta aaaaatggtg atcattacat tttaaacgga
    10561 agcaaacagt ttacaacaca cggaaccgtt ggtgaaacat atgttgtaat ggcaattacc
    10621 aataaagagg ctggcaaaaa agggatctct gcattcattc ttgaaaaagg aatggaagga
    10681 ttaattattg ggaagaaaga aaataaactt ggaatgcgtg ccagcgatac aactcaatta
    10741 gcttttgaaa attgtaaagt cccggcagaa aatcttttag gcgaagaagg aatgggattt
    10801 attaattcaa tgaaagtttt agaaggcgga agaatttcta ttgctgcttg ttcaattgga
    10861 ttagcacaag gttgttt
//
LOCUS       DASWBY010000204         3690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_82456, whole genome shotgun sequence.
ACCESSION   DASWBY010000204 DASWBY010000000
VERSION     DASWBY010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82456"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..864)
                     /gene="truA"
                     /locus_tag="VF870_03755"
     CDS             complement(136..864)
                     /gene="truA"
                     /locus_tag="VF870_03755"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX9251329.1"
                     /translation="MKNYKLIIQYDGTNYSGWQIQKNSISIQQKITEELETLLKDKIN
                     LIGSGRTDSGVHAIGQAANFRTEKEIDIYRFKHSLNSMLPSDIVVTKMEEVDSEFHAR
                     FDAKKRTYLYLITQSRSPFFKNYSYFYPRKIDIEKLNSLSKNFLGEKDFTSFSRKNSE
                     IENKDCNVYKIFWRKKGELILFSIQASRYLHGMVRTVVGTLLNAQDQNNPEDFIQNIF
                     NSQNREEAFEAVPAKGLFLYKVEY"
     gene            1087..3027
                     /locus_tag="VF870_03760"
     CDS             1087..3027
                     /locus_tag="VF870_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9251330.1"
                     /translation="MKTSFKLTSEKIGGKTEVQKETLDKYRSLYENHPLIHITINFDG
                     SIISVNQNGAKELGYSVDELINTHISNLFIPKEAARLEKQIQYVLLNPEKQSSHEMKM
                     RRKNKQEFWVRETIYTTENSSLQKEIFFVFDNISYQKNAEENAKNLAQSLQNMLDCSP
                     LGVLVYRLDENDDLILISTNQSAVNILQIDVYALISKKIQDIFPALVKDNFVGKFKDV
                     LNSGIPLLNQNIIYEDIHFKGIYEFSVMKLSDKIIAIFFTDVTEKQKALEALKQSELK
                     FKTLFESANDAIFLIKEDVLTDCNEKTTQLFKTTKIDLIGKNPFLFSPTVQPDGQSSK
                     TKFKEKVAQTMEGIPQFFEWKYTSLDGSQFDAEISLKRVELQNEFYLQAIVRDITDRK
                     ISERIISEQKRELDTLMSNLPGMAYRCANNISWTMEFVSEGCYQLTGYKKDELLNDKV
                     VSFANLIHKDDQLLVYETVQNAVNNHESFTLMYRINTWQGLEKWVWEKGQAIYDDNGD
                     VICLEGFITDITERKLAEEKINILAHALKSVSECVCITNMRDKIMFINKSFSRVYGYS
                     QNEIIGKPISFIRSTKNDPAIVKKIFPETLAGGWNGELIYKRKNGEEFQAHLSTSLIT
                     DDAAKPIALASVSIDISDSKSR"
     gene            3212..3679
                     /locus_tag="VF870_03765"
     CDS             3212..3679
                     /locus_tag="VF870_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX9251331.1"
                     /translation="MSHYIHDDVVITEKELLREIVNLKEKLFELESERRNQKKIVNQY
                     KSLFPKITSPMFLTDVEGKIIDANKEFMDFLGYAIFDLKRMTIKSIVPMKNHENETKL
                     LLEELEINKHITSRWCEFFKINGEKIQAELEVFATFDENNRFDGITRIVKNIL"
BASE COUNT         1365 a          544 c          578 g         1203 t
ORIGIN      
        1 ctgctgatgc aggatttgaa ttataagtaa aagcaacaga tgcaccggct atacaaaaat
       61 attttacaca cgctgcgcca atgcctctcg atccgcctgt aataaaaatt ttctttgagt
      121 ttaggtttaa catttttaat actccacttt atataaaaat agtcccttag ccggaacagc
      181 ttcaaaagct tcttcccgat tttgtgagtt aaatatattt tgaataaaat cttcaggatt
      241 attttgatcc tgtgcattta ataaagtgcc gactactgtt ctaaccattc cgtgcagata
      301 cctgcttgct tggattgaga acaagatcag ctcacctttt tttcgccaaa agattttata
      361 cacattacaa tctttatttt ctatctcact attctttctg ctgaatgaag taaaatcttt
      421 ttcacctagg aaatttttgc tcagagaatt taatttttct atatcaattt tacgtggata
      481 aaaatatgaa taatttttaa agaaaggtga tcgggattgt gttattaagt acaaatatgt
      541 tctttttttt gcatcaaatc ttgcatgaaa ttctgaatca acttcttcca ttttagtaac
      601 tacaatgtct gaaggcagca ttgaatttag tgaatgctta aatctataga tatctatttc
      661 tttttcagtt ctaaaattag cagcttgccc aattgcatga acacctgaat cagttcttcc
      721 cgatccaata agattaattt tgtcttttag taaagtctcg agttcttctg taattttttg
      781 ttgaatagaa atagaatttt tttgaatctg ccaacctgag taatttgttc catcatattg
      841 tattataagc ttatagtttt tcaaaaacag atttccagat gtttaaattt aatatcgtaa
      901 agataacccc ggaggtaaat tataacaatt ataattttta taaggtataa attaccagta
      961 attaaatcca ctgtatacaa attattaaaa tccagcactt aacaataaaa gataatctta
     1021 ttatcttata aggtaagttt ttatgtttat ttgattctaa atcaaaaatc ataaattaag
     1081 tatcttatga aaacttcatt caaattgacc tcagaaaaaa taggcggaaa gactgaagtg
     1141 caaaaagaaa ctttagacaa ataccgatct ctttacgaaa atcatccatt aattcatatt
     1201 acaattaatt ttgatggaag tatcatatct gttaaccaaa atggcgcaaa agaattaggc
     1261 tacagtgttg atgagttaat aaatacacat atctcaaatt tatttattcc aaaagaagct
     1321 gcacggttag aaaaacaaat ccagtatgtt ttgttaaatc cagagaaaca atcttcgcat
     1381 gaaatgaaga tgcggcgcaa aaataaacag gaattttggg taagagagac aatttataca
     1441 acagaaaatt cttctcttca aaaggaaata ttttttgtgt ttgataatat ttcttatcaa
     1501 aaaaatgctg aagaaaatgc aaaaaatctt gcacaaagtc ttcagaatat gcttgattgt
     1561 tctccattgg gtgttctggt ttacagatta gatgagaacg atgatttgat tttaataagt
     1621 acaaatcaat ccgcagtaaa tattcttcaa atagatgttt atgctttaat atcaaagaag
     1681 atccaggata tttttccagc tttagtaaag gataactttg tcggcaaatt taaagatgta
     1741 cttaatagcg gcatacctct cttaaatcaa aatataattt atgaggatat acactttaaa
     1801 ggcatctatg aattttcagt aatgaaatta tctgataaaa taatagccat attctttact
     1861 gatgttaccg aaaagcaaaa agcgcttgag gctttgaagc aaagtgagct aaaatttaaa
     1921 acactttttg aatctgctaa tgatgccata tttttaataa aagaagatgt tttgactgat
     1981 tgtaatgaga aaacaactca attatttaaa acaacaaaaa ttgatttgat tggaaaaaat
     2041 ccttttctat tctctcctac tgtgcagcct gacggacagt cttctaaaac aaaatttaag
     2101 gaaaaagtag cccagacaat ggaaggtatt ccacagtttt ttgaatggaa atataccagt
     2161 cttgatggat cacaatttga cgctgagata agtttaaaaa gagttgaact tcaaaatgag
     2221 ttttatctcc aagcaatagt cagagacata actgatagaa aaatatctga aagaattatt
     2281 tcagaacaaa aaagagaatt agatacgctt atgagcaacc ttcccggaat ggcgtacaga
     2341 tgtgcaaata atatcagttg gacaatggag tttgtgagcg aaggctgcta tcaattaaca
     2401 ggttacaaaa aagatgaact attaaatgat aaagttgtca gctttgccaa ccttattcac
     2461 aaagatgatc agttacttgt ttatgaaact gtgcaaaatg ctgtaaacaa ccatgaatct
     2521 tttacattaa tgtaccgtat aaatacttgg caaggacttg aaaaatgggt atgggaaaag
     2581 ggtcaagcta tttacgacga caatggtgat gttatttgtc ttgaaggttt tataacagat
     2641 ataacggaaa gaaaattagc cgaagaaaaa ataaacatcc tggcccatgc attaaaaagt
     2701 gtatcggaat gcgtttgcat aaccaatatg cgtgataaaa ttatgtttat aaataaatca
     2761 tttagccgtg tttacggtta ttcgcaaaat gaaattattg gaaaaccaat ctcatttatc
     2821 agatctacaa aaaatgatcc agccattgtt aagaaaatat ttcctgaaac tttagctggc
     2881 ggatggaacg gtgaacttat ttataaaaga aaaaatggtg aggagttcca agcacatctt
     2941 tctacttcgc tgattactga tgacgctgct aaaccaattg cactagccag tgtaagcatt
     3001 gatatttctg attcaaaaag cagataatga ctcagtctat tgattaaaat taattactgg
     3061 tagcaactat ttgtgatggt tcaattattt tagtgcaaaa aaacgggttt catattaaaa
     3121 atagtgcaaa ctaactattt actcttccac ttaattattt ttacttatag taaataatta
     3181 aataatttaa acatttatta tggggacaaa tatgagccac tacattcacg acgacgttgt
     3241 tataactgaa aaagaattac tcagagaaat tgttaattta aaagaaaaac tatttgaatt
     3301 ggaatctgaa agaagaaacc agaaaaaaat agttaatcaa tacaaaagtc tattcccgaa
     3361 aattacaagc ccaatgtttc ttacagatgt tgaggggaaa ataattgatg ccaataaaga
     3421 gtttatggat tttctaggtt atgcaatttt tgatttgaaa agaatgacca tcaaatccat
     3481 cgtccctatg aaaaaccatg aaaatgaaac caaactttta cttgaagaac ttgagataaa
     3541 taaacatatc acctcacgct ggtgtgaatt ctttaaaatc aatggagaaa aaattcaggc
     3601 agagcttgaa gtttttgcaa cttttgatga gaacaaccgg tttgatggaa ttacaagaat
     3661 agtaaaaaat attttgtaat tatttaacat
//
LOCUS       DASWBY010000205         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_572982, whole genome shotgun sequence.
ACCESSION   DASWBY010000205 DASWBY010000000
VERSION     DASWBY010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572982"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VF870_03770"
     CDS             <1..491
                     /locus_tag="VF870_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEX9251332.1"
                     /translation="IDSIFTPIKLVKYDVENVRIGDKNDFEKLTLEITTDGSITPDDA
                     LTQAAKILREHVQLFINFDIEQEEEASVSQKDSETERIKKILLTPVDDLELSVRSHNC
                     LKAANIKNLAELVRKDEQEMLKFRNFGRKSLAELMEIVETLGLDFGMDVDKYIKEDSE
                     SH"
     gene            509..856
                     /gene="rplQ"
                     /locus_tag="VF870_03775"
                     /pseudo
     CDS             509..856
                     /gene="rplQ"
                     /locus_tag="VF870_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            1062..1646
                     /gene="yihA"
                     /locus_tag="VF870_03780"
     CDS             1062..1646
                     /gene="yihA"
                     /locus_tag="VF870_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX9251333.1"
                     /translation="MFENQKFILSAFSTAELPKEKLPEVVLCGRSNVGKSSFINSLFN
                     RKNLAKTSSTPGKTRSINYYLIDSKFYVVDLPGYGYAKISQSERKKWGLLINDFFKTS
                     NHIQLTIHLIDSRHQPTDLDIKLNDMLRSLEIPYIVLLNKSDKLKQSEFSTAKKRIIE
                     IFPELILNENLFYFSSIKGTGNKEIKKLLTALFY"
     gene            1737..>2506
                     /locus_tag="VF870_03785"
     CDS             1737..>2506
                     /locus_tag="VF870_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9251334.1"
                     /translation="MDKRQKKRILIFLIIPVLAIILFITDDLLIRVITIAVIIIYVAF
                     IIFLRDSVRFDGRYSISEKDELDDEYKTPPVSEHDESFKIVSKTSKVEVITSENYIPE
                     FKTPKTTLIPPDLRERFEEIANESLPPEIGHDGQFSFALEKMLTVIKDAYSAHTAIFF
                     WYNKKKDKLTIEKFVSSSPNEIAKRKFDIEDDILSKIVQKGEPELLSDISPAAESDVI
                     RYYDKPQGIRSFVGVPLFYEGSLIAIIAVDAKVGDSFG"
BASE COUNT          936 a          345 c          440 g          785 t
ORIGIN      
        1 caattgattc aattttcact ccaattaagt tagttaagta tgatgttgag aatgttagaa
       61 ttggtgataa aaatgatttt gaaaaattaa ctttagaaat cactactgat ggttcaatta
      121 cacctgatga tgctcttacg caagcagcta aaatattaag agagcatgtt cagttattca
      181 ttaactttga tattgaacaa gaagaagaag caagtgttag tcaaaaagac agcgaaactg
      241 agagaatcaa aaagatactc ttaactccgg ttgatgatct tgaattaagc gtaagatcac
      301 acaattgtct taaagctgct aatataaaaa acttagcaga gctagttaga aaagacgagc
      361 aggaaatgct caagtttaga aatttcggaa gaaagtcctt agcagagtta atggaaatag
      421 ttgaaactct tggtcttgat tttggaatgg atgtagataa atatattaaa gaagattctg
      481 aatcacatta atttagatag gttgctcaat gagacacagc gttaaaggaa gaaaattagg
      541 cagaactgca agtcatcgtt cagcattgct aaatgcctta acgacttctc tgttaaaata
      601 taaaagaatt agaactaccg aagctaaagc caaggaagca agaacttttg tagaaaaatt
      661 aattactaaa gctaagaaaa atgatcttca cgttcgacgg caggtaatgg cttgtattaa
      721 tgataaagaa gttgttaaag aactttttag cgaaatcatc cctaagattg gtgaaagacc
      781 gggtggttat acacgagttg taaagctagg caatcgtaat ggtgatgctg catcaatggc
      841 tattttagaa ttggtagact ataatgatgt ggctaacaaa aaagctgaag aacataaagc
      901 taaacgtgaa ttaaaggcta aagataaagc tgataaaaaa ggcggcagcg aaatagaaga
      961 agctactgtg gttgaagaaa aaaccagcaa aaaaagtaaa taaaaaatag tttatattat
     1021 tactgaaaga gtaaccggaa ttccggttac tcttttttat tatgtttgaa aatcaaaaat
     1081 ttatcctttc cgccttcagt actgctgaat tgccaaaaga aaaactacca gaagtggttt
     1141 tatgtggtag atctaatgtt ggcaaatctt ctttcattaa ctcgcttttt aatagaaaaa
     1201 atcttgcaaa aaccagttca acaccaggta aaacaagatc aataaattat tacttgattg
     1261 actcgaaatt ttatgtagtt gatttgccgg gttacggata tgcaaaaatt agtcaatcgg
     1321 aacgaaaaaa atggggtttg ctgataaatg atttttttaa gacttcaaat catattcagt
     1381 taaccatcca tttaatagat agccgccatc agccaacaga tttagacatt aaattaaatg
     1441 atatgcttcg atctttggaa attccatata tcgttttatt aaataaatct gataaattaa
     1501 aacaatctga attttctact gctaaaaaac gaattataga aatttttccg gaacttattc
     1561 ttaacgaaaa tctcttttac ttctcatcaa ttaaagggac aggaaataaa gaaattaaaa
     1621 aactacttac agcattattt tattaaatag tttatatctt tgctacaaag agtttgtaat
     1681 ttttttaaca tagatatata ttaactaagt attaatcatc tgtaaaacta cttctgatgg
     1741 ataaaagaca aaagaaaaga atacttatat ttttaattat acctgtcctg gcaattatac
     1801 tatttattac agatgatttg ctgatacggg taataactat tgccgttatt attatttatg
     1861 ttgccttcat tattttttta agagattcag ttagatttga tgggcggtat tctattagtg
     1921 aaaaagatga attagatgat gaatataaaa caccccctgt aagtgagcat gatgaatcct
     1981 ttaagattgt atctaaaact tctaaagttg aagtaataac atccgaaaac tatataccag
     2041 aatttaaaac ccctaagacc acgcttattc ctccggattt acgggaacga tttgaagaaa
     2101 tcgctaatga atcattacct cccgaaattg gacatgatgg acaatttagt ttcgcgttag
     2161 aaaaaatgtt gacggtaatt aaggatgctt actctgcaca tactgctatt ttcttttggt
     2221 acaataagaa gaaagataag ttaactattg aaaaatttgt gtcatcttct cccaatgaaa
     2281 tagccaaaag aaaatttgat attgaagacg atattttaag caaaattgtt caaaaaggtg
     2341 agcctgaatt attaagcgat atttcgccag ctgcagaatc agatgtgatt agatattacg
     2401 ataagccgca aggtataaga agttttgttg gcgttccatt gttttatgaa ggttcgttaa
     2461 ttgcaataat tgctgttgat gctaaagttg gtgattcatt tggaat
//
LOCUS       DASWBY010000206         7946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_158869, whole genome shotgun sequence.
ACCESSION   DASWBY010000206 DASWBY010000000
VERSION     DASWBY010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158869"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VF870_03790"
     CDS             complement(<1..953)
                     /locus_tag="VF870_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9251335.1"
                     /translation="MANGKKKKSKKKLYIFGGIGLLLVILLVVAFVGGNKEEIVSVQT
                     EKVEKRTITQTVAATGKINPEFKVVITPEVTGEIVELPVKEGDVVKKGQLLIRIKGDQ
                     YQAQKQRLEANLQSAEANLKMREAELTKVELDYNRVKELYSKGLSSDSELETAKSNYL
                     TTKASYEAAEANVLQSKASLRETLETLYKTTIVSPMDGVVTKLNVELGERVFGAGFSM
                     GTDIMTISDLRNIEAVVDVDENDVVLVSIGDTANIKVDAFKDQEFTGLVSEIGNSANT
                     TGLGSQNEVVNFEVKIKLLNPKTSLRPGMSCTADIETETIK"
     gene            complement(980..2326)
                     /locus_tag="VF870_03795"
     CDS             complement(980..2326)
                     /locus_tag="VF870_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9251336.1"
                     /translation="MKLFLASILLLISANSLFAQSELTLDQAIRIAIQKNSGLLTQEN
                     ELASGESGVTAAYGNFLPTLGLNAGWGWNRNDQAERTAVVDGANIKFPAGYSESRNYY
                     VGANSNVVLFDGLSNIANLSKSKNNVEAIKLSIEAKKQEVVFQTISLYYSITESYQLL
                     KVREEDVKQQQKNLETITERNRLGSVTMADVYQQQVQLGNAELEVIRANNALETAKSN
                     LLYYLGLDVLENYTYSKYLTDRELAILAQNIDTDYNRLNDLVKEALENRRDYQAQKLN
                     LNSLYDNLTIARSGHLPSLVGNINFNAPANTVPEIFDSRNLNIGLNLNIPIFSGFSVD
                     NAVQIAEVNVMNSEIELKDTERFIKQNLQKSFLDLEAAKKLLVVSEKNVKAAEENLKI
                     EQEKYNLGAGKLLDVLIANTAFQNAKTNYINAQFFYIRTSDELQYNLGVLDYLKYE"
     gene            2607..3032
                     /locus_tag="VF870_03800"
     CDS             2607..3032
                     /locus_tag="VF870_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251337.1"
                     /translation="MKSCLSNASNKILIFGLFLSLLICDLSYSQRQNKIFEDIGGGSG
                     TTNTNVESNDDYTLYIVGGLIVTGIVIYALLRDKKEKPTKDTATVSLNEDYLEKNLSY
                     NEKIANLQSQIPINITFGIQRDKALKDEKRYFVGLAYNF"
     gene            complement(3049..4464)
                     /locus_tag="VF870_03805"
     CDS             complement(3049..4464)
                     /locus_tag="VF870_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX9251338.1"
                     /translation="MNQGSEILVKEKISQLEIVNAISSTAERENVKVYAVGGFVRDII
                     LNRDRNDLDILVIGSGPDFAKSAAEKLGISNVSYFKNFGTAHFEYSGMNIEFVGARKE
                     SYDRNTRKPIVEDGTFKEDISRRDFTINTLAISLNKNEFGKLIDKYDGLNDINNKLIR
                     TPLDPLKTFDDDPLRIMRAFRFASQLNFNVDPGIMIAANEMKDRLSIVSQERITDEFL
                     KILTTPKPSIGLKLLFDSNVLGVVFPEIAVMAGVDQRRDYHHKDVFLHTLEVVDNICK
                     ETENVWLRFAALVHDIAKPPTKKFVEGIGWTFHGHEDLGAKMMKKIFHRMKLPLHQLE
                     FIQKLIRLHLRPIALAKEEVTDSAIRRFVVEAGEDLEDLIVLCRADITSKNPVKVEKY
                     LGNYERVMQKVRDVKERDQLRAFQSPVRGEEIMQICNLRPSRKVGEIKTAIEEAILEG
                     KIGNNYEEAYKYLMEIKDQFL"
     gene            complement(4494..6647)
                     /locus_tag="VF870_03810"
     CDS             complement(4494..6647)
                     /locus_tag="VF870_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251339.1"
                     /translation="MAIILINVILTQLPLTSTFGYEFASFNGLLLAIIAGLQTFNFID
                     KSEFVVKELIKNLAILFLIPFFISVIHSFLTTFCSFTDGLIFYVLIVSTSMVFGTSIA
                     MVISLIAKRFRRLFFFIIIFLIALIPILEIYLNPQVYFYSPLIGFFPGNIYDEGLTAD
                     WKLFFHQFVIIIFSLSIIYSVLNLPANVKRIRILFLAAILIVFALFQYLSPYLGFTTT
                     FSKLNSILPKQIESESLVLHYDGIDSLNAKIIALNQEFYYSELSTQLGVKPANKINVY
                     LFNNREQKKIIFGAGNADVAKPWQYSIYISAGSWQSTLKHELAHVFTAEFGSGIFKLA
                     SGFNAALIEGMAESLDGISNDISIKDLTSIAFNNGYVVDIKSLFSGLSFFKGNSSLAY
                     TYSGTFVQYLTQKYGIEKAKEFYRDGDFESVFRIKIDTAQKEFEKSIINPELSNQRLM
                     ADYYFGRLSIIQKVCPRYIGDRLSKAYKYLVENNYDESEHLFKEINNKTLNYSALIGL
                     SEIYYKQNKKDEAINLILNNYNKFKSTSYYHNLIFRLAELYALDLQYDSAAFYYNKII
                     NDNVSQQLNYLCNTRLSLIEGNKLFEYVDGSDSLKLEILISLNQKEYNYNSLPIIIDL
                     IQNQNIDYKKSLSIFNKRFIVNNIISSFAAFRLSNYMLANNDFINARKYAALSLRYKE
                     NNMFYTAMQEQFEKADWLVKNSIKFLDKFVYVSKD"
     gene            complement(6745..>7946)
                     /locus_tag="VF870_03815"
     CDS             complement(6745..>7946)
                     /locus_tag="VF870_03815"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX9251340.1"
                     /translation="AMNKSSDNNFFNAAEFKDAVNNFNLSYSQSRENNLTFPDDSEIE
                     TIHSGKKTSKGFQTVTNILLFIIFIGLGVVVFNVVKSIVLQEEAKTDSKALSYTQDYS
                     KNPNFVKESGWELIKVDTDNNLNSIAFIDDYRGFIVGDSGLTMQSSDGGNTWKKINSS
                     FQNNFNSISFIDNKLLVVGSSGFIGLLNSNSNQIKKIYSNTSEALFKIYFNNSNNGFI
                     VGSTGLILKTNDGGLTWQKIPSNVNENLFSISFADSKNGIIVGWNGTILKTEDGGLTW
                     RRQKIKTNSYLKDVLFVNEFLGFIAGGEGKIFRTENGGDSWDEIDMESNSGLYKVSFD
                     SNGEGIVLSNRGEIFYSYDAGKTWAKKTVGQPLILNDIKKLNSGNYIIVCNSGNIFKS
                     KVSPVNK"
BASE COUNT         2581 a         1423 c         1084 g         2858 t
ORIGIN      
        1 tccttaatag tttctgtttc tatatctgcc gtacaactca taccaggtct taagctagtc
       61 tttgggttaa gaagttttat tttaacttca aaatttacaa cttcattctg actacctaat
      121 ccggttgtat tagcgctatt accaatttcg gaaactagtc ccgtgaattc ctgatcttta
      181 aaagcatcaa ctttaatatt ggcagtatca ccaattgaaa ctaacacaac atcattttca
      241 tctacatcca ctacagcttc gatatttctt aaatctgata ttgtcataat atctgtaccc
      301 atactaaatc ctgcaccaaa aactctttca ccaagttcaa cattaagctt ggttacaact
      361 ccatccatag gtgaaacgat tgtggtttta tacaatgttt caagagtttc tcttaacgaa
      421 gctttacttt gtagtacatt tgcttcggcg gcttcataag aagctttggt agttaagtaa
      481 tttgattttg ctgtttccag ttcagagtcg cttgataaac ctttgctata aagttccttt
      541 actctgttgt aatccaattc aactttggtc aactcagctt ctctcatctt taaatttgct
      601 tctgcagatt gaagatttgc ttccaacctt tgtttttgtg cctggtactg atcgccttta
      661 atgcgtatta gcagttgacc tttctttaca acatcacctt ctttaaccgg cagttctaca
      721 atttcacccg tcacctcagg agttataaca actttgaact caggatttat ttttccagtg
      781 gctgccacag tttgggttat agtccttttt tcaacttttt cggtttgaac agaaactatt
      841 tcctctttat tacctccaac aaaagctact actaaaagta ttacaagcag caaccctatt
      901 ccaccaaaga tgtataattt ctttttcgat tttttctttt ttccattagc catttgagac
      961 attctcctat gattaaaatt tattcgtatt ttaagtaatc taacacgcca agattgtact
     1021 gcaattcatc agatgtacgt atgtagaaaa attgtgcatt aatataattt gttttagcat
     1081 tttgaaaagc agtatttgca attagaacat ccagtaattt gccagcacca agattatatt
     1141 tttcctgttc gatcttaagg ttttcttctg cagctttaac atttttttct gaaacaacca
     1201 ataatttttt tgcagcttca agatcaagaa atgatttttg cagattttgt tttataaatc
     1261 tctcagtatc cttcaattca atttctgaat tcatcacatt cacttcagca atttgtacag
     1321 cattatcaac cgagaatcct gaaaaaatcg gaatgtttaa attcaaacca atgttaagat
     1381 tacgcgaatc aaaaatttcc gggaccgtat ttgcaggggc gttgaaatta atattcccaa
     1441 caagacttgg aagatgtcct gatcgggcaa ttgttaaatt atcgtataat gaattaagat
     1501 ttaatttttg cgcctggtaa tcacgtctgt tttctaaagc ttcttttact agatcattta
     1561 aacgattata atctgtatca atgttctgag ccaaaatagc aagttctcta tctgttaaat
     1621 attttgaata agtataattt tctaaaacat ctaatcctaa atagtagagc agattacttt
     1681 ttgcagtctc taaagcatta tttgcgcgaa tcacttccaa ttcagcatta ccaagctgca
     1741 cttgttgctg ataaacatca gccattgtta cagatccaag tctgtttctc tcagtaattg
     1801 tttcaagatt tttttgctgc tgctttacat cttcttctct aacttttaat agctggtatg
     1861 attcggtgat tgaataatat aaactaattg tctgaaatac gacttcctgt ttttttgctt
     1921 ctatcgaaag tttaattgct tctacattat tcttactttt ggaaagattt gcaatatttg
     1981 atagtccatc aaacaaaaca acatttgaat ttgcaccaac ataatagttt cttgattcgc
     2041 tatatccagc gggaaattta atatttgctc cgtccacaac agcagttctt tcagcttgat
     2101 catttctgtt ccatccccaa ccggcattta atcctaatgt gggaaggaaa tttccatacg
     2161 ctgctgttac tccagattcg ccgctggcta attcgttttc ttgtgtcaat aaaccgctat
     2221 ttttttgaat tgcaattctt atagcctgat caagtgttag ctcactttgt gcaaataaag
     2281 aatttgctga tattaaaagt aaaatagatg ctaagaataa tttcatttgt ttctccgaat
     2341 gttggattga aaattcatgc tttctgtaaa aatacgacaa taattagatt ctatctttga
     2401 ttttgaccac tcaaaaatag tttatttacc aaccaaatga ttatataatg tataaatggt
     2461 taaataatta catctgcggt atatattagc aaataacaat aaagattgat taatagttcg
     2521 tatttactta ataatgtaat tctaggttga ttctgagaaa gtaaatggtt tatattcaca
     2581 ttgtaaaaat tctaaaagag gcatcaatga aatcttgctt atcaaacgct tcaaacaaaa
     2641 ttctaatttt cggattattt ctatcacttt taatttgtga tttaagttat agccagagac
     2701 aaaataaaat cttcgaagat attggcggag gatcgggcac tacaaatacc aacgtggaat
     2761 ccaatgacga ttatactttg tacattgttg gcggtttgat tgttaccgga attgttattt
     2821 atgctctatt gcgcgataaa aaagaaaaac caactaaaga tactgctact gtctcgctta
     2881 atgaggatta tcttgagaag aatctttctt acaatgaaaa gattgcaaac ttacagtcac
     2941 aaatccctat aaatattaca tttggaatac aacgggataa agcgcttaaa gacgagaaaa
     3001 gatattttgt tggtttggct tataattttt aacagcccgg aatcagtttt ataaaaactg
     3061 atctttaatc tccattaaat acttataagc ttcttcataa ttgttgccga ttttaccttc
     3121 aagaattgcc tcttcgatag cagtttttat ctcccctact tttcttgaag gtctgagatt
     3181 gcagatttgc ataatttctt ctccccttac aggcgattga aatgctctta attggtctct
     3241 ctcttttaca tctctaacct tttgcataac acgttcgtag ttgccgagat atttttcaac
     3301 tttaacaggg tttttactgg taatatcagc acgacataaa acgattaaat cctcaagatc
     3361 ttcacccgcc tccacaacaa atctacgtat tgctgaatct gtaacttctt ctttggccaa
     3421 agcaattggt cttaaatgca atctgataag cttttgaata aactcaagtt gatgaagcgg
     3481 taatttcatt cgatgaaata ttttcttcat catttttgca ccaagatctt cgtgaccatg
     3541 aaacgtccag ccaattcctt caacaaattt cttcgttgga ggttttgcga tatcatgaac
     3601 aagtgcagca aaccttaacc aaacattttc ggtttcttta caaatattat caaccacttc
     3661 aagtgtatgt aaaaaaacat ctttatgatg ataatctctt ctttgatcta caccggccat
     3721 gacagcaatt tcaggaaaaa caactcctaa aacattggaa tcaaaaagaa gttttaaacc
     3781 gattgatggt tttggtgtgg tcaatatttt aagaaactcg tctgttatcc tctcctgtga
     3841 tacaattgat aatctatcct tcatctcatt tgcggcaatc atgattcctg gatcaacatt
     3901 aaaattaagt tgagatgcaa aacgaaatgc acgcattatt ctcagtggat cgtcgtcaaa
     3961 agttttaagg ggatcaagcg gagttctaat taatttatta tttatatcgt ttaagccatc
     4021 atatttatca ataagttttc caaactcatt tttgttaaga gaaattgcta aagtgttgat
     4081 tgtaaagtcc cgtcgtgaaa tatcctcctt aaaagttcca tcttcaacta taggttttct
     4141 ggtattacga tcgtatgatt cttttctggc gccaacaaat tctatattca ttcctgaata
     4201 ttcaaaatga gctgtgccaa aatttttaaa gtacgataca tttgaaatac ctagtttttc
     4261 agctgcactt tttgcaaaat caggtccact gccgatcact aaaatatcca aatcatttct
     4321 atcacgattt aatattatgt cacgaacaaa cccgccaaca gcgtaaactt taacattctc
     4381 tctttctgca gtgctagaaa tagcattaac aatctccaac tgagagattt tttctttaac
     4441 tagtatttca gatccttgat tcattacaat tattaaaatg agaatttttt aatttaatcc
     4501 ttcgaaacat aaacgaattt atctaaaaac ttaatagaat tttttaccag ccaatcggct
     4561 ttttcaaact gctcctgcat tgcagtataa aacatattat tctctttgta ccgtaaactt
     4621 aatgcagcat actttctggc attaataaaa tcattatttg caagcatata attagaaagt
     4681 ctgaacgcag caaaactgct aatgatatta ttaactataa atcttttatt aaatatactt
     4741 agtgattttt tataatcaat attctgattt tgtattaaat caataataat gggtaatgaa
     4801 ttataattat attctttctg attcaatgag atcaatattt ctaactttaa tgaatcactc
     4861 ccatctacat attcgaataa tttattgcct tcaattaaac taagccttgt attgcacaag
     4921 taatttaatt gctgactaac gttatcatta attattttat tatagtaaaa agctgctgaa
     4981 tcatattgta aatcaagtgc gtacaattca gctaatctga aaatcaaatt atggtaataa
     5041 gatgtagatt tgaatttatt ataattattt aaaattaaat ttatcgcttc atctttttta
     5101 ttttgcttat aatagatttc agaaagtcca atcagtgcgg aataattaag agttttatta
     5161 tttatctctt taaacaaatg ttctgattca tcataattat tttcaactaa gtatttataa
     5221 gccttactta atcgatcacc gatgtatctt ggacatactt tttgaataat tgatagtctt
     5281 ccgaaataat aatcagccat aagtctttga tttgataatt caggattaat tatgctcttt
     5341 tcaaattcct tttgcgctgt atcaatcttt attctgaata cggattcaaa atctccatcc
     5401 ctataaaact cttttgcctt ttcaattcca tatttctgag tcagatattg tacaaaagtt
     5461 ccgctgtaag tatatgccaa agaagagttt cccttaaaaa aactcaaccc tgaaaacaaa
     5521 gattttatgt ctaccacata tccattatta aatgctattg aagttaaatc ctttattgaa
     5581 atatcattgg atattccatc aagtgattcc gccatccctt caattagcgc tgcattaaat
     5641 ccagaagcaa gtttaaatat ccccgatcca aattctgctg taaagacatg tgctaattca
     5701 tgttttaacg tgctttgcca agagcctgca ctgatataaa ttgaatattg ccatggtttc
     5761 gctacgtcag catttccggc accaaagatt attttcttct gttctcggtt attaaataag
     5821 taaacattta ttttatttgc tggtttaact cccaattgag ttgatagttc tgaataataa
     5881 aattcctgat taagtgcgat gattttagca ttcagggaat caattccatc atagtgcaga
     5941 actaacgatt cagattcaat ttgctttggt agaattgaat ttaattttga aaaagtagtt
     6001 gtaaatccta gatatggaga taaatactga aataatgcaa atacaattaa aatcgcagct
     6061 aagaataata tcctaattct ctttacattt gccggcagat ttaaaactga ataaataata
     6121 gaaagtgaaa atattattat cacaaattga tgaaaaaaca atttccaatc tgcagttaga
     6181 ccttcatcat aaatatttcc aggaaagaag ccaattagag gcgaataaaa ataaacctgc
     6241 gggtttaagt atatctctaa tattgggata agtgcaatca gaaaaattat tataaaaaag
     6301 aagagccttc taaacctttt agcaatcaaa cttattacca ttgcgattga cgtaccaaaa
     6361 accattgaag tacttacaat caagacataa aatattaatc catctgtaaa tgaacaaaac
     6421 gttgtaagaa atgaatgtat aactgagata aagaatggga ttaaaaatag gatcgccaga
     6481 ttttttataa gttctttaac aacaaattct gatttgtcaa taaagttaaa tgtctgaaga
     6541 ccggcaataa tcgcaagtaa cagtccatta aatgaggcaa attcataacc gaatgtactt
     6601 gttaagggta actgagtcag gatgacattg attagaatta ttgccaatac gtagactaat
     6661 aacccacttt tatatgataa tatttttttt aaaaacattt ttagtttcaa ttttgtagac
     6721 acggcgattt tataaatatc aaacttattt atttactggg ctgaccttac ttttaaagat
     6781 atttccactg ttgcagacaa taatataatt acccgaatta agctttttga tatcatttaa
     6841 aattaatggc tgcccaacgg ttttttttgc ccacgtcttc cctgcatcat aggaatagaa
     6901 aatctcacct ctatttgaca gcactattcc ttcgccattg ctatcaaaac ttactttata
     6961 taaaccggaa tttgattcca tatcgatttc atcccaactg tctccaccgt tttctgtacg
     7021 aaatatttta ccttcaccgc ctgcaatgaa tcccaaaaat tcatttacaa ataaaacatc
     7081 ttttaaataa gaattagttt tgattttttg tcttctccag gtcaatccac catcttcagt
     7141 tttaagaatc gtcccattcc atccaacgat aattccattt ttagaatcag caaaactgat
     7201 gctgaataaa ttttcattca cgttacttgg aattttttgc caggtcaatc cgccatcatt
     7261 ggtttttaaa atcaatccgg tgctaccaac tataaacccg ttattagaat tattaaagta
     7321 aatcttgaac agtgcttcag aagtattaga atatattttt ttgatctgat ttgaattact
     7381 atttaatagt ccaataaatc ccgatgagcc aacgacaagt aatttattat caataaaact
     7441 tatagaatta aaattatttt gaaagctgga attaattttt ttccaagtgt ttccgccatc
     7501 cgaagattgc attgttaacc cggaatctcc tacaataaat cctctataat catcaataaa
     7561 tgcaattgag ttaagattat tatctgtatc aactttaatt aattcccacc cgctttcttt
     7621 tacaaaattt ggattttttg aataatcctg agtataactt aaagcttttg aatccgtttt
     7681 cgcttcctcc tgtaagacta ttgattttac aacattgaaa actactacac ctaatccgat
     7741 aaatattatg aataacaaaa tgttagtaac tgtctgaaaa ccttttgaag tttttttacc
     7801 tgaatgaatt gtttctatct ctgaatcatc ggggaaagtt aaattatttt ctctgctctg
     7861 tgaataactt aaattaaaat tatttacagc atctttaaac tcggcagcat taaaaaaatt
     7921 attatcagaa gatttattca tcgctc
//
LOCUS       DASWBY010000207         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_123007, whole genome shotgun sequence.
ACCESSION   DASWBY010000207 DASWBY010000000
VERSION     DASWBY010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123007"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VF870_03820"
     CDS             complement(<1..837)
                     /locus_tag="VF870_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9251341.1"
                     /translation="MQNIRISDLGKFVGQEVTLQGWLFNKRSSGKIKFIILRDGSGYL
                     QCVYFKGNLTEEVFDKADKIGQESSIEVTGKVKEEPRAPGGYELDATDLKIISEAHDY
                     PITPKDHGIEFLLDNRHLWLRSSKQVAIMKIRHRIVKAIRDFFDERGFTLMDPPILTP
                     NAVEGTSTLFETPYFDLGNAYLTQSGQLYAEAGAMALGKVYTFGPTFRAEKSKTRRHL
                     TEFWMVEPEVAFNDLNDNMDLAEEFLEYIVQTILKEKSEELKVLERDITKLQNVKRPF
                     PRV"
     gene            complement(973..1620)
                     /locus_tag="VF870_03825"
     CDS             complement(973..1620)
                     /locus_tag="VF870_03825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9251342.1"
                     /translation="MIKNYKHIIWDWNGTLLNDVDYCCKIINKILVKNFLPELSLEKY
                     RQIFTFPVQDYYKGAGLDFTKTSFEILGKDFIDQYEANKLSCSLFENAIEILSAIHKK
                     GIGQSVLSAYLHDNLVKILDHYKLTKYFDNIIGLDNIYAGSKTHLGLSLVEQLNMPKN
                     EILFIGDTLHDAEVAEAMSVNSILISNGHQTKNKLLINNNIVMDNLNQLQEFFDL"
     gene            complement(1604..2278)
                     /locus_tag="VF870_03830"
     CDS             complement(1604..2278)
                     /locus_tag="VF870_03830"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9251343.1"
                     /translation="MNYLDKLEKVITQNCSIIAKELYLNSAVLIPIVFLNDTEFVLFQ
                     KRSSTVRQPGEVSFPGGHYDSNCDKDFLSTAIRETCEELGIEKSKIIVIGSIGTLVGQ
                     MGVIVEAYLGILKINSLNELKLDHKEVDRVFLIPLEYFINNKPDEYFTRLEIHPYLIK
                     EDGEKVELLPVKELGLPEKYSLPWTQGRHRVLVYKTSEEIIWGITAELIFELSNKLKE
                     KIDKKL"
BASE COUNT          740 a          424 c          322 g          830 t
ORIGIN      
        1 aacgcgaggg aaaggacgtt ttacgttctg aagttttgtt atatctcgct ctaaaacctt
       61 taactcttca gatttttctt ttaaaattgt ctgaacgata tattctaaaa actcttcagc
      121 cagatccata ttatcattta aatcgttaaa tgcaacttca ggttcaacca tccaaaattc
      181 agttaaatgt cttcttgttt ttgatttttc tgcacggaaa gtcggaccaa atgtataaac
      241 tttgcctaat gccattgcac cggcttctgc atatagctgt cctgattgcg taagataagc
      301 gttacctaaa tcaaaatatg gagtttcaaa aagagtagat gtaccttcaa cagcatttgg
      361 agtaaggatt ggaggatcca ttaaagtaaa accgcgttca tcaaaaaaat cacgaattgc
      421 ttttactatg cgatgacgga ttttcattat tgcaacttgt ttactagatc gcagccataa
      481 atgtctgtta tcaagcaaga attctatacc gtgatctttt ggtgttattg gataatcatg
      541 tgcttctgaa ataatcttca aatcagttgc atcaagttca tatccacccg gagcgcgagg
      601 ttcttctttt acttttcctg taacttctat tgatgattcc tgaccaatct tgtcagcttt
      661 atcaaaaacc tcttcggtca aattaccttt aaagtaaacg cattgaagat agcctgagcc
      721 atcgcgaagg attataaact taattttgcc gcttgagcgc ttattgaaga gccatccctg
      781 aagtgtaact tcctgcccta cgaatttccc aagatcactg attctaatat tctgcattaa
      841 tttcgttttt tctttgaaaa ttgactgcca aatatataga gagggtctaa aaaaaccaat
      901 caattacaat ttcattttac ttttgaaaaa gagaactaaa tggtttatct gattagcctt
      961 caaactaatg ttttacaaat caaaaaattc ctgcaactga ttcaaattat ccatcacaat
     1021 gttattattt atcagcagct tattttttgt ttgatgacca tttgaaatta gaattgagtt
     1081 aacactcatt gcttctgcta cttcagcgtc atgtaatgta tccccgataa ataaaatttc
     1141 attcttaggc atattcagct gttctacgag tgataatccg agatgtgttt tactaccagc
     1201 gtaaatgtta tcaagcccaa ttatattatc aaaatattta gtgagtttat aatgatccag
     1261 aatctttacc agattatcat gtaaatacgc ggatagtact gactgcccaa tacctttttt
     1321 gtggattgct gataaaattt ctatcgcatt ttcaaacaat gaacaagata atttattcgc
     1381 ttcgtactga tctataaaat ccttacctaa tatttcaaaa gaggttttag taaaatctaa
     1441 acctgctcct ttataataat cctgtacagg aaatgtaaaa atctgtctgt acttttctaa
     1501 agataactcc ggtaaaaaat ttttaacaag aattttatta ataattttgc agcaatagtc
     1561 aacatcattt aataatgttc cgttccaatc ccaaataata tgtttataat tttttatcaa
     1621 ttttttcttt cagtttgtta gataattcaa aaataagttc agcggtaata ccccaaatta
     1681 tttcttcaga tgtcttgtaa accaatacac gatgccttcc ttgagtccag ggtaatgaat
     1741 atttttcagg taagcccaat tctttaaccg gaagcaattc tactttttcc ccatcctctt
     1801 ttatcaaata tggatgaatt tcaagtctgg taaaatactc atcaggtttg ttgtttataa
     1861 aatattctaa tggaattaaa aatactctat caacttcttt atgatctaat tttaattcgt
     1921 ttaacgagtt aatttttaat attccaagat atgcctctac aattactccc atttgtccta
     1981 ccaatgttcc aatacttccg ataacaataa ttttactttt ttctattccc agttcttcac
     2041 aagtttctct aattgctgtg gaaagaaaat ctttatcgca atttgaatca tagtgtccac
     2101 caggaaaact aacttcaccg ggttgcctta cagtggaaga tcttttttgg aaaagtacaa
     2161 attcagtatc atttaaaaaa actatcggta tcaacacagc cgaattaaga tacaattcct
     2221 ttgcgataat agaacaattt tgtgttatta ctttttcgag tttatcaaga taattcattt
     2281 ccctaattca aatcattttt tatagtagga gcaaat
//
LOCUS       DASWBY010000208         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_9514, whole genome shotgun sequence.
ACCESSION   DASWBY010000208 DASWBY010000000
VERSION     DASWBY010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9514"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /gene="tsaE"
                     /locus_tag="VF870_03835"
     CDS             <1..369
                     /gene="tsaE"
                     /locus_tag="VF870_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX9251344.1"
                     /translation="EFALKLKNGMIIILNGDLGAGKTFFIKHVLKKFNVTNSNSPTFA
                     IVNEYSGDKTFYHFDFYRINKESELHDIGIEDYFNDEQSIIFIEWGNLFPDVLPKKRI
                     EISISYIDENQREFNFIEYE"
     gene            362..1036
                     /gene="tsaB"
                     /locus_tag="VF870_03840"
     CDS             362..1036
                     /gene="tsaB"
                     /locus_tag="VF870_03840"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX9251345.1"
                     /translation="MNNILPLLAIETSDSICGACVYFGEENFFSSKLVLKHSHAEKLF
                     ELINYVLKTASIAQNELKLIAVSNGPGSFTGLRIGMSAAKGVAQALSIPIIPVPTFEA
                     LALQITNFLPDSSEFFIANKVGRDEVYLAKFQIMANNYIFKEELKIVPVSELNLIPKN
                     ILVFGNAFNSNTKEAINSKSISAPDPEYIAKWAVAFGIEKIVNDIDFIEPNYLKDFIV
                     KEKKND"
     gene            1029..1745
                     /locus_tag="VF870_03845"
     CDS             1029..1745
                     /locus_tag="VF870_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEX9251346.1"
                     /translation="MIRNTAIIIIFSVISVFPQLIAPKVGVQQPNYDFGTINQGVVVS
                     HSFVVSNNGGDLLKILDVKAACGCTAAKPEKSELKPGESTNIAVSFNSKGRKGPQTKT
                     VTVTTNDSEKPQVTFTFTCNVVVKEVNESTAGAKIFFPETQHDFGKVPEGKKVEYTFK
                     FENKGTDALVIKDVKTSCGCTAAVVSNSTLKPGEVGSIKVDFDTKSRQGRNSKSITVI
                     SNDSKEPNKIITIYADVQKN"
BASE COUNT          641 a          267 c          325 g          574 t
ORIGIN      
        1 gaatttgcgt taaaattaaa gaacggaatg atcatcattt taaatggtga tcttggtgca
       61 gggaaaactt tttttataaa acatgtcctg aagaaattta atgtaactaa ttctaatagt
      121 ccgacctttg caatcgtaaa tgagtattcc ggagataaga ccttctatca ttttgatttt
      181 taccggataa ataaagaatc tgaattacac gatattggga ttgaagatta ttttaatgat
      241 gagcaatcaa taatatttat agaatgggga aatttgtttc cggatgtgct tccgaaaaaa
      301 agaattgaaa tatccatcag ttatattgat gaaaaccaaa gagaatttaa ctttatagaa
      361 tatgaataat attttaccgt tgcttgcgat tgaaacctca gattcaattt gcggtgcttg
      421 tgtttatttt ggcgaggaaa attttttttc ttcaaaatta gttttaaaac attcgcatgc
      481 agaaaagtta tttgaattga ttaactatgt tctaaagacg gcatcaattg cacaaaatga
      541 attgaaattg attgctgtat caaacggacc tggctccttt acaggtttaa gaatcggaat
      601 gtctgcagcc aaaggtgttg cacaggcatt gtcaattcca attattcccg ttccaacttt
      661 tgaagcactg gccctgcaga tcactaattt tctgccagat agttccgagt tttttattgc
      721 aaataaagtt ggcagagatg aagtatattt agcaaagttt caaattatgg ctaataatta
      781 tatatttaag gaggaattaa aaattgtacc tgtttcggaa cttaatttaa ttcctaagaa
      841 tattttagtt tttggaaatg cattcaattc aaatactaag gaagccataa actctaaaag
      901 catttccgct ccagatccgg aatatatcgc aaagtgggct gttgcttttg gaattgaaaa
      961 aattgtaaat gatatcgatt ttattgaacc aaattattta aaagatttta ttgttaagga
     1021 gaaaaaaaat gattagaaac actgcaataa ttatcatatt tagtgtgata tctgttttcc
     1081 ctcaacttat tgccccaaaa gttggtgttc aacagcccaa ttatgatttt gggactataa
     1141 atcagggtgt tgttgtaagt cattcattcg ttgtttcaaa taatggcgga gatttattaa
     1201 aaattcttga tgttaaagcg gcttgtggat gcactgctgc taaaccggaa aaaagcgaat
     1261 taaaaccagg ggagtcaacc aatattgctg tatcatttaa ctctaaaggc agaaaaggtc
     1321 cacagactaa aacagtaact gttactacaa atgattctga aaaacctcag gtaactttta
     1381 catttacctg caacgtagtt gttaaagaag ttaatgaaag taccgctggt gcaaaaatat
     1441 ttttccctga aacgcagcat gattttggta aagttccgga agggaaaaaa gtcgaataca
     1501 cttttaaatt tgaaaacaaa ggaaccgacg cgcttgttat taaagacgta aaaacttcct
     1561 gcggatgcac agccgcagta gttagtaata gtactttaaa acctggtgaa gttggatcga
     1621 ttaaagttga ttttgataca aaaagtcgtc aaggcagaaa cagcaaatca attacagtta
     1681 tttccaatga ttccaaagaa cccaacaaaa taataaccat ttatgccgat gtgcagaaga
     1741 attaattatg ccttggttta aaagatcaaa acaaaatatt gctgatagta ctcagcaaaa
     1801 agaatta
//
LOCUS       DASWBY010000209         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_158982, whole genome shotgun sequence.
ACCESSION   DASWBY010000209 DASWBY010000000
VERSION     DASWBY010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158982"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..607
                     /locus_tag="VF870_03850"
     CDS             248..607
                     /locus_tag="VF870_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9251347.1"
                     /translation="MKKTTTETRAYRTEMNDKILNSGFRDFNKFFALDNKAYIDGALN
                     AKTKELMGLSASMVLRCNDCIFYHIDRSIQEGATREELMETFNVALIVGGSIVIPHLR
                     YAFEIMDKIFEEESKTE"
     gene            607..1944
                     /locus_tag="VF870_03855"
     CDS             607..1944
                     /locus_tag="VF870_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9251348.1"
                     /translation="MIIVPKNIVTVDGKDRILNNHFVEIENGKIIRIAKSDEKYFKRY
                     KNDVYKFENLTLIPGFVQTHIHLCQTLFRGLADDLELLDWLQLKIFPYENAHNKSSLL
                     SSVRLGINELLLSGTTTLLDMGTLRHQEVIFDELQNSGMRATAGKCLIDENDLFPEFK
                     SNTKSELDEIYQLAKTYNNTSNGKIKYGFAPRFVLSCTEKLLKESFEMKKDFEGSLYH
                     THSSENKSEIAEVKRRHNKENIEYFDSIGVIDDHSVLAHCIHVNDKETNLLKKKDVRV
                     AHCPSSNMKLGSGIANIPKYLNKGISVSLGADGAPCNNNLSVFNEMRLAALIQKPIHG
                     PLAMDAKTVFKLATIEGAKALHLQNEIGSIEVGKKADLVLMDLNTKVHSISEGYESIY
                     SEIVYSSSADSVNSVMIEGEWKVLNGGSILYDQQELNLKSKEELKKLLKRAQN"
     gene            1941..2519
                     /gene="rdgB"
                     /locus_tag="VF870_03860"
     CDS             1941..2519
                     /gene="rdgB"
                     /locus_tag="VF870_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX9251349.1"
                     /translation="MKKQILLATTNKGKLHDVQEILKGIDVKILSFLNFSDYPNVEET
                     GTTFLENAELKAKAAFEKYGIPSFGDDSGLEAFQLNREPGIYSARYAGEEADDEKNNL
                     KLIKNLSELPEPHLGRFVCAAVYYDGKDFKSAFGEVRGNIIKSPRGKNGFGYDPLFIP
                     NGYEKTMAELTHEEKNKISHRLNAFKELKKYL"
     gene            2516..>2701
                     /locus_tag="VF870_03865"
     CDS             2516..>2701
                     /locus_tag="VF870_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251350.1"
                     /translation="MKKSLAVIFSLVVLLNACKESTTEPTVDVTSSDYFPNSKGNYYH
                     YNFSVYDSSGLIQSGTRK"
BASE COUNT          995 a          393 c          507 g          806 t
ORIGIN      
        1 cacaaaaata tgattgggaa gatgaagaat cagacactcc ctcaaacaag ttggaagtat
       61 cgtacgatgc tgataaaaat aaatttttag atgagttttg aaaatcttgg tcattgtgag
      121 cgagtcttcg aacaaagcaa tctcataatt ttgtcatcag aaattcagat tgcttcaccc
      181 actgaagtgg gattcgcaat gacatattaa gtgagaataa ataaattaaa aagttttagg
      241 aaagaaaatg aaaaaaacta caacagaaac gcgtgcatac cgtacagaaa tgaatgacaa
      301 aattctaaac tcaggattca gggattttaa caaattcttt gctcttgata acaaagctta
      361 cattgatggg gcactcaatg ctaagacaaa agagcttatg ggattgtctg catcaatggt
      421 tctgcgttgc aacgattgca tattctatca cattgatcgt tcaattcagg aaggcgcaac
      481 tcgtgaagaa ttaatggaaa catttaatgt cgcattaatt gttggcggct caattgttat
      541 tccacatctc cgctatgcat ttgaaataat ggataaaata tttgaggaag agagtaaaac
      601 agaataatga ttattgttcc caaaaatatt gtaacagttg atggaaaaga cagaattctt
      661 aataatcatt ttgttgaaat tgaaaatgga aagataattc gaattgcaaa aagtgatgag
      721 aaatatttta agagatataa aaacgacgtt tataaatttg aaaatcttac tttaattccc
      781 ggatttgtac aaacacatat tcatctttgc caaactctgt ttcgtggttt agcagatgat
      841 cttgagcttc ttgattggct gcaattaaaa atatttccat atgaaaatgc acataataaa
      901 agttcgcttt taagttccgt aagattaggc ataaacgaac ttttattaag cggcacaaca
      961 acattgcttg atatgggaac attgcggcat caggaagtaa tttttgatga actgcagaat
     1021 tccggaatgc gtgcaactgc tggcaaatgt ttgattgatg aaaacgattt gttcccggaa
     1081 ttcaaatcaa atacaaaatc agagcttgat gagatttatc aactagcaaa aacatataat
     1141 aatacttcaa acggtaaaat taaatatggc tttgcaccaa gatttgtttt atcgtgtaca
     1201 gaaaaacttc taaaagaatc ttttgagatg aagaaagatt ttgaaggaag tttatatcac
     1261 actcactcat ctgaaaataa aagtgaaatt gctgaagtca aacgaagaca caataaagaa
     1321 aacattgagt actttgattc aattggtgta attgacgatc attcagttct tgcacattgc
     1381 attcacgtta atgataaaga aactaatctg ttgaaaaaga aggatgtgcg tgttgcacat
     1441 tgtccttcat ccaatatgaa acttggttct ggaatcgcga atattccaaa atatttaaat
     1501 aaaggaattt cagtttctct tggggcagac ggtgctcctt gtaataataa cctaagtgtc
     1561 tttaacgaaa tgagattagc agctctgatc caaaaaccaa ttcatggacc cttggcaatg
     1621 gatgcaaaaa cagtatttaa acttgcgacc attgaaggcg caaaagcatt gcatcttcaa
     1681 aatgaaattg gaagtattga agtgggtaag aaagcagatt tggtattgat ggatttgaat
     1741 acaaaagttc attcaatatc tgagggttat gaatcaattt attctgagat tgtttattca
     1801 tcatcagcag attcagttaa ttcagttatg attgaaggag agtggaaggt tttgaatgga
     1861 ggttcaattt tgtacgacca acaagaactt aacttaaaat caaaagaaga actaaaaaaa
     1921 ctattaaaga gagctcagaa ttgaaaaaac aaattcttct tgctacaaca aacaaaggta
     1981 agctgcacga tgttcaagag attctgaaag gcattgatgt aaaaatttta tcgtttctga
     2041 acttttcgga ttatccaaat gttgaagaaa cagggacaac atttttagag aatgctgaat
     2101 taaaagcaaa agccgctttt gaaaaatatg gcattccaag ttttggtgat gattcagggt
     2161 tagaggcctt tcagttgaat cgtgaaccgg gaatttattc tgcacggtac gcaggtgaag
     2221 aggcggatga tgaaaaaaat aatttaaagt tgattaaaaa tctatctgaa ttacctgaac
     2281 ctcacctggg aagatttgtt tgtgcagctg tttactatga tggaaaagat ttcaagtcag
     2341 catttggaga ggtaagagga aatattataa aatctcctcg cggtaaaaac ggatttggtt
     2401 atgatccatt atttatacca aatgggtatg aaaaaaccat ggcagaacta actcatgaag
     2461 agaaaaacaa aatcagccat agattaaacg catttaagga attaaaaaaa tatttatgaa
     2521 gaaaagttta gcagtaatat tctctctggt tgttttatta aatgcttgta aagaatctac
     2581 aactgaacct acagttgatg tgacctcatc cgattatttc cccaattcca agggtaatta
     2641 ttatcactat aatttttcag tttacgatag ttcaggatta attcaatccg gtacaagaaa
     2701 a
//
LOCUS       DASWBY010000210         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_82641, whole genome shotgun sequence.
ACCESSION   DASWBY010000210 DASWBY010000000
VERSION     DASWBY010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82641"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VF870_03870"
     CDS             <1..541
                     /locus_tag="VF870_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX9251351.1"
                     /translation="FYPYLRDENLARPWAIPGTPDLEHRIGGLEKANIYGTVNYDPDN
                     HNKMIHLRAEKIKYIENDIPLLEVDGDQTGDLLVLGWGGTYGAIKEAVIKARSLGMNI
                     SQAHLQYVNPFPKNTGEVLRNFKQVLIPEINLGQLAKLLRSEYLVDTVQFNVVRGLPF
                     RVSDILEKIKETLGGKNGK"
     gene            531..1418
                     /locus_tag="VF870_03875"
     CDS             531..1418
                     /locus_tag="VF870_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX9251352.1"
                     /translation="MENKVEVKTAKLTAKDFASDQEVRWCPGCGDYSILAQVQRTFPE
                     IINVPKEDVVWISGIGCSSRFPYYMDTYGFHGIHGRAPILATGVKLANPKLSVWVATG
                     DGDLLSIGGNHFIHACRKNIDIKILLFNNRIYGLTKGQYSPTSEKGKVTKTTPFGSVD
                     YPFNPTMLATGSEATFVARSIDRNTKHLQEMIKRVGAHKGTAFLEIYQNCNIFNDGAF
                     SVLTEKETKDDNVLVLEHDKPMVFGKDSSKGIRLDGFTPRVVDLTSGQYSVNDLWIHD
                     EFEESYVRSAILSSFTETP"
BASE COUNT          526 a          250 c          331 g          461 t
ORIGIN      
        1 attttatcct tacttgcgtg atgaaaatct tgcaagacct tgggcaattc ctggaacacc
       61 ggatttggaa cacagaattg gtggacttga aaaagcaaat atttacggaa ctgttaacta
      121 tgatccggat aatcataaca agatgattca tttgcgagca gaaaaaatca agtatattga
      181 aaatgatatc ccgcttcttg aagttgatgg cgatcaaaca ggcgatttac ttgttttagg
      241 ttggggcgga acttacggcg ctattaaaga agcagtgatc aaagcgcgaa gtttaggtat
      301 gaatatttca caggctcatc tgcagtatgt aaatccattc cctaaaaata caggtgaagt
      361 attgaggaac tttaaacaag tgcttattcc tgaaattaat ctgggtcaac tagcaaaact
      421 tttaagaagc gaatatttag ttgacactgt tcaattcaat gttgtacgcg gattaccgtt
      481 cagagtatca gatattttag aaaagattaa ggaaacactc ggaggaaaaa atggaaaata
      541 aagttgaagt aaagactgct aagttaactg ctaaggattt tgcatctgat caggaagtaa
      601 gatggtgtcc gggttgcggt gattactcaa tccttgcaca ggttcaaaga acttttcctg
      661 aaataattaa tgtgcctaaa gaggatgtag tttggatttc cggaatcgga tgttcaagtc
      721 gttttcctta ctacatggat acttatggtt ttcacggtat tcacggcaga gcgccaattc
      781 ttgcaacagg agtaaaactt gcaaatccaa aactttctgt ttgggttgca acaggtgatg
      841 gtgacttact aagtatcggt ggaaatcact ttattcatgc ctgcagaaaa aatatcgata
      901 tcaaaattct attattcaat aatcgtattt atggattaac taaaggacag tattcaccaa
      961 catcagaaaa aggaaaagtt acaaagacaa ctccatttgg tagtgttgac tatcctttta
     1021 atccaacaat gcttgcaacc ggttctgaag caacattcgt agctcgttca atagatagaa
     1081 atacaaagca cttgcaggaa atgatcaaac gggttggtgc gcataaaggg acagcatttt
     1141 tagaaatata tcagaactgt aatatcttta atgacggtgc tttctcagtt cttacagaaa
     1201 aagaaactaa agacgataat gttttagttc ttgaacacga taagccaatg gtttttggga
     1261 aagattccag caaaggaatt agacttgatg gattcactcc aagagttgtg gatttaacga
     1321 gcggacaata ttcagttaat gatttatgga tacatgatga gtttgaagaa agttacgttc
     1381 gttctgcaat tctttcatca ttcaccgaaa caccgtgatt cccaacacca attggtgttt
     1441 tcagacaagt attcaaaccg acttatgatg aaggtttagt tgctcagatt gaggatgtta
     1501 aaaagaaaaa aggtgtaggc gagttagaaa aagtgctttt tggcggcaat acctgggaag
     1561 ttaattga
//
LOCUS       DASWBY010000211         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_573275, whole genome shotgun sequence.
ACCESSION   DASWBY010000211 DASWBY010000000
VERSION     DASWBY010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573275"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="VF870_03880"
     CDS             complement(<1..1974)
                     /locus_tag="VF870_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9251353.1"
                     /translation="MLKKLLLAIITIASLNFSQTIQDLIQPINLLQDQPTKVLVSDMF
                     YAENYNLEFTSSKNITVTYDASTNEVTFSPKNNFSGLELIPFKFNGEIYQVPVKLVKS
                     PKYLFTYKPQPGEKQVNLFGQFNSWDRQGLPMVDSNGDGILEVEIPLDPGRYEYKFYV
                     DGREVVDPVNPDKVPNGMGDFNSLRIIEESAKDKMFLHVLGSKKVKNDLKLNFYFENI
                     DKNMRVDKKSLIVLFDNKIFPSELIQINGREITLTVKGKMLAGNHSVRIAVNRMGKNS
                     NIQTVQIHDGVIAGKSDVHTLNDNIIYSMMIDRFNNGDKSNDNPIVHDSLFSPANYQG
                     GDLQGIINKMEEGYFDKLGVNAFWISPIVDNTNNAYREYPAPHRWYTGYHGYWPVSST
                     KVEEHFGDMNLVKKLIDIAHKKDSKVFLDYVAHHVHQEHWMWRDHRDWFGTYNLPNGR
                     LNLRLWDEYRLTTWFEPYMPSFDFVGSYEALEYMTDNGVWWLKVTGADGYRHDAVKHV
                     PNEFWRLLTKKIKCEIATPENKTIYQIGESFGGIDMIASYVNNGQLSAQFNFNLYDVA
                     VPTFLDEKASFKLLDYQMQKSFQVFGYNNLMGNIMDSHDKIRYMAYADGDLAINDGRA
                     GDFAWNNPPKVDDPKSYDKLKLHLTYILTIPGIP"
     gene            complement(2107..2520)
                     /locus_tag="VF870_03885"
     CDS             complement(2107..2520)
                     /locus_tag="VF870_03885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9251354.1"
                     /translation="MSEIIYTVEKSLDVSEFIEVLKKSKLAERRPIDDEQRISSMCNN
                     ANLIVTARLDGILIGVARSITDFVYCTYLSDLAVDIKYQKTGIGKRLIEETKEQTPQA
                     KLILLSAPAAIDYYPKIGMTKHNHCYYIDDVKDLK"
     gene            complement(2592..>2950)
                     /locus_tag="VF870_03890"
     CDS             complement(2592..>2950)
                     /locus_tag="VF870_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9251355.1"
                     /translation="SFEIKYIYFRFVIKQHSLIKKRGLFMAAKPMTKGQILDHMAKKT
                     GVTKKLAGQFLDELVALSYKEAKKSFTFPGLGKLVVVNRKKRMGRNPATGATMVIPAK
                     RVLKFRIAKAAKDNAL"
BASE COUNT          910 a          550 c          456 g         1034 t
ORIGIN      
        1 tggaatacca ggaatggtta atatgtaagt aagatgtaat tttagtttgt cataactttt
       61 aggatcatca actttgggtg gattgttcca tgcaaaatca cctgctctgc catcattaat
      121 tgcaagatcg ccatcagcat acgccatata acggattttg tcatgactat ccataatgtt
      181 gcccatcaga ttgttatatc caaaaacctg aaaacttttc tgcatctgat agtcaagtaa
      241 tttgaacgaa gctttttcat caagaaatgt tggaacagca acatcataaa gattaaaatt
      301 aaactgcgca cttaactgtc cgttgttaac ataggaggcg atcatatcaa ttcccccgaa
      361 cgattcacca atctgataaa tcgttttatt ttcaggagtt gctatttcac atttaatttt
      421 ttttgttagc aatctccaga attcatttgg aacatgctta actgcatcgt gcctgtagcc
      481 atcagcacct gtaactttta accaccagac accattatcc gtcatatatt caagggcttc
      541 gtaagagccg acaaaatcaa aggaaggcat ataaggctcg aaccaggttg ttaatcggta
      601 ctcatcccat agtcttaaat tcaatcttcc atttggcaga ttataagttc caaaccaatc
      661 acggtgatct ctccacatcc agtgttcctg atgaacatga tgtgcgacat aatccagaaa
      721 aacctttgaa tcttttttat gagcaatatc tattaatttt ttcacaaggt tcatatcacc
      781 aaaatgctct tcaacctttg ttgatgaaac cggccaataa ccgtgatagc ccgtatacca
      841 tctgtgagga gcgggatact cacgataagc gttgtttgtg ttatcaacaa tcggagaaat
      901 ccagaatgca tttacaccaa gtttatcaaa atatccttct tccattttat taatgattcc
      961 ctgtaaatct cctccctgat aatttgcggg actaaaaaga gaatcgtgta caattggatt
     1021 atcattagat ttatcgccgt tattaaaacg atcaatcatc atactgtaga ttatgttatc
     1081 atttaaagta tgaacatctg atttaccggc aattactccg tcatgtattt gaactgtttg
     1141 aatgttacta ttctttccca ttctgttaac tgcaattcta acagagtgat ttccagctaa
     1201 cattttacct tttacagtca gagtaatttc tcttccgttt atttgtataa gttctgatgg
     1261 aaaaattttg ttatcgaaaa gaacaataag acttttttta tcaacccgca tgtttttatc
     1321 gatgttctcg aaataaaaat ttaactttaa atcattttta acttttttcg atccaagaac
     1381 atgaagaaac attttatctt ttgctgattc ttcaattatc cttaacgaat taaaatcacc
     1441 cattccattt ggaaccttat ccggatttac aggatcaaca acttctcttc catcaacata
     1501 aaacttgtat tcatatctgc cgggatcaag cggaatctct acttcaagta tgccatcccc
     1561 atttgaatct accattggta agccctgtct atcccagcta ttaaattgtc cgaataagtt
     1621 tacttgtttt tcaccaggct gtggtttgta agtaaaaaga tatttgggac tttttacaag
     1681 tttaacagga acctgataaa tctctccatt aaatttgaat ggaatcaatt ccaatcctga
     1741 aaaattattt ttcggagaaa aggttacttc gttcgttgaa gcatcataag ttactgtaat
     1801 attcttgctt gatgtaaact ctaaattata attctcagca taaaacatat cactaaccaa
     1861 aacttttgtt ggttgatcct gcaaaagatt aattggctgt attaaatctt gtatggtttg
     1921 agaaaagttt agcgaggcaa tggtgataat ggctagtaaa agttttttta acatttttag
     1981 ttacctgtag tgttgtccaa ttattttacg ttgaatagtt ccggttggtc tatttagacc
     2041 cgaatctttt tttacaaggt aaatttacga taaagacatc aaaagattaa gtgcgaaaag
     2101 ccatgattat tttaaatctt taacatcatc aatataatag caatgattat gttttgtcat
     2161 tccgatttta ggataataat caatggcggc aggagcggat agtagaatta gttttgcctg
     2221 tggagtttgt tccttggttt cttcaattaa tctttttcct attcccgttt tctgatattt
     2281 tatatcaacg gcaaggtcag aaaggtaagt acagtaaacg aagtctgtaa ttgagcgggc
     2341 aacaccaatt aatattccat ctaatcttgc agtaacaatc aggttagcat tattgcacat
     2401 tgatgaaatt ctttgttcgt catctatcgg tctgcgttca gcaagtttgc ttttcttaag
     2461 tacttcaata aattccgaaa catcaagcga tttttcaacc gtgtaaatta tttcgctcat
     2521 aaaatatcct tataataaaa aagcgaagaa catttctatt cctcgcttta ataaaataat
     2581 cttaatccag attataaagc gttatctttt gctgctttag ctattctgaa ttttaacact
     2641 ctctttgcag gaataaccat tgtagcgcct gtagctgggt ttctgcccat tctcttcttg
     2701 cgattaacta caactaattt acctaaacct ggaaatgtga atgacttctt agcttctttg
     2761 tatgataaag ctacgagctc atctaaaaac tgaccagcta attttttggt aacgccggtc
     2821 ttctttgcca tatgatcaag aatctgacct ttggtcattg gttttgctgc cataaatagc
     2881 cctctctttt ttattaatga atgttgttta attacaaagc gaaaataaat atattttatt
     2941 tcaaatgaaa
//
LOCUS       DASWBY010000212         3449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_9625, whole genome shotgun sequence.
ACCESSION   DASWBY010000212 DASWBY010000000
VERSION     DASWBY010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9625"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VF870_03895"
     CDS             <1..985
                     /locus_tag="VF870_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251356.1"
                     /translation="FSQGINPKGKGYYGKTFYYQTPLNAKAVKSNFYDSIKKVNSFIK
                     KEKKDLLLSKTKSEICVGLYKPYFYTELTNSQLLKEKRLDVEKLGLSLDPRFVREDLF
                     FNGLLRGLQTLNFNYDISDLENTSLESLLKYKQLWIVTTEFMDSKTQLLLANYIKAGG
                     HLIMYPALPTKDLYLNPCMVLKQELKIEFSKSVSPNKVNAFGIEDLFTIFKDKQVFAD
                     NDSDVVAITESDEVCGIKKKVGKGVITLLGFAFGYSSDEHLHLYEKLVALDKIKRQAK
                     VSDSDIQFVIRKGKKSSYLFLLNYHNQKKTFTVDSKKTVLSPFSYKVIKKN"
     gene            999..2891
                     /locus_tag="VF870_03900"
     CDS             999..2891
                     /locus_tag="VF870_03900"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX9251357.1"
                     /translation="MIRKKMKFFWMISICVSIATIMTKAQIDLVAPLVPQGVQAFGYE
                     KNVDVEWYNNSEPDLAGYKIYKWNGTQYTFYTTVTKEKSYLTLNVGVLGVSYSFKISA
                     YDLNGNESDLSDSVDAITHEMTDEEFLDMVQRSTFRYFYDYGHPVSGLSRERLGSGET
                     VTSGGSGFGLMVLLVGIERGYITREQGVERMLKILNFLKNNADKFHGAFSHWLNGTTG
                     AVIPFGQYDDGGDLVETSFMIQGILTVRQYFDQVNTNEEQIRNLCTEIWEGVEWSWYR
                     RTSFSNYLYWHWSPNYGWIINFKLIGWMETMITYMLGIASPTYSVPARLFHDGWASSN
                     YTNSNTYYGYRLWVGPSYGGPLFFAHYSFLGFDPRDKKDAYCNYFMNNKNHTLINRAY
                     CIANPYNHPGYGPNNWGLTASDEPNGYSAHAPYSNDNGTITPTAAISSMPYTPTESIE
                     ALKNFYRTYGSNVWGEYGFKDAFNPRQNWFASSYIAIDQGPIIVMIENYRSGLLWEKF
                     MANPEIQPMLDSIGFVPDSVTSVDEKVKTVNDFELLGNYPNPFNPSTTIVFNLNANEN
                     VKIEIFNHLGERVSEVSNKEFSAGENKVVWNGLNDENKNVSSGIYLYKISTAQSSLYG
                     KMILQK"
     gene            2894..>3449
                     /locus_tag="VF870_03905"
     CDS             2894..>3449
                     /locus_tag="VF870_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9251358.1"
                     /translation="MRLSFRKLIVFLLPIVMVLFSCSKENESEKTIKFWAMGAEAEYV
                     IKLVPEFERLNPGIKVKVQQVPWTAAQEKLVTAFASDNTPDACQLGNTWVPQFSALNA
                     IIPLDEFIKSSNQINKEKYFTGIWETNVLDDQIFGIPWYMDTRVMFYRKDVFEKAGYE
                     NPPKTWAELLDLSIKIKAMHPKEEK"
BASE COUNT         1199 a          480 c          667 g         1103 t
ORIGIN      
        1 gttctcgcag gggataaatc caaagggtaa aggctattat ggaaaaactt tttattacca
       61 aactccgcta aacgctaaag ctgttaagtc aaacttctat gattcaataa aaaaagttaa
      121 ttcgtttatt aaaaaagaaa agaaagattt attattaagt aaaactaaat ctgaaatatg
      181 cgtcggttta tataagccct atttttatac agaacttaca aattctcaat tacttaaaga
      241 aaaaagactt gatgttgaaa agcttggtct ttctcttgat ccaaggttcg ttagagaaga
      301 tttatttttt aatggattac tgcgagggct tcagactctc aattttaatt atgatataag
      361 tgatttggaa aatacttcgc ttgagtcttt attaaagtat aaacagctct ggattgttac
      421 aaccgaattt atggatagca aaacccagct tttacttgca aattatataa aagccggcgg
      481 acatttaata atgtatccgg cacttccgac aaaggattta tatcttaatc cttgtatggt
      541 actaaagcag gaattaaaaa ttgaattttc taaatcagta agtccaaata aagttaatgc
      601 ttttggaatt gaagatttat ttacaatctt taaagacaaa caagtatttg cggataatga
      661 ttctgatgtt gttgcgataa cagaatcaga tgaggtttgc ggtataaaaa agaaagttgg
      721 gaagggagtt ataacattac tcgggtttgc atttggttac tcaagtgatg agcatctaca
      781 cttatatgaa aaattagttg ctttggataa aataaaaaga caggcaaaag tttctgattc
      841 tgacattcaa tttgtaataa gaaaaggaaa gaaaagttca tatttgtttt tattaaacta
      901 tcataaccag aaaaaaacat ttacagttga ttcaaagaaa acagttttaa gtccgtttag
      961 ttataaggta attaagaaaa attagtaaag agaaaaaagt gataagaaaa aaaatgaaat
     1021 tcttttggat gatatctatt tgtgtttcaa tcgcaacgat aatgactaaa gctcagatag
     1081 atcttgtcgc accattagtc ccgcagggtg ttcaggcatt tggttatgaa aaaaatgttg
     1141 atgttgaatg gtataataac agtgagcctg atcttgcagg atataaaatt tataagtgga
     1201 atggaacaca gtatactttt tataccacgg taacaaaaga aaaaagctat cttaccctta
     1261 atgttggagt tttgggggta agttattcat ttaaaattag tgcttatgat ttaaatggaa
     1321 acgaatcaga tcttagtgac agtgttgatg caattacaca cgaaatgact gatgaagaat
     1381 ttttagatat ggttcagcgt tcaacattca gatattttta tgactacgga catcctgttt
     1441 ccggtctatc aagagaacgt ctaggttctg gtgaaacagt tacaagcggt ggttctggtt
     1501 ttggtttgat ggtattactt gttggtattg agagaggata tattactcgt gagcagggtg
     1561 ttgagcgtat gctaaagatt ttaaactttc taaaaaataa tgcggataaa tttcatggtg
     1621 ctttctctca ttggcttaac ggaactaccg gtgctgttat tccttttggg cagtatgatg
     1681 atggcggcga tttggttgaa acttctttta tgatacaagg tatattaact gttcgccaat
     1741 attttgatca agtaaatact aatgaagaac aaattagaaa tctttgcaca gaaatttggg
     1801 agggtgttga gtggagttgg tatcgaagaa caagctttag taattatttg tactggcact
     1861 ggtcaccaaa ctatggctgg ataattaatt ttaaattgat tggctggatg gagactatga
     1921 taacttatat gctgggaata gcatcaccaa cgtacagtgt tccagcaaga ttatttcacg
     1981 acggttgggc gagttcaaat tatactaata gcaatactta ctatggctat agattatggg
     2041 ttggaccaag ttatggcgga ccacttttct ttgcacatta ttcatttcta ggattcgatc
     2101 cacgcgataa gaaagatgct tattgtaatt actttatgaa taataaaaat catacgttaa
     2161 taaatagagc ttattgtatt gcaaatcctt ataaccatcc tggatacgga cctaacaact
     2221 ggggactaac tgcaagtgat gaaccaaatg gatatagcgc tcacgctcct tacagtaatg
     2281 ataatggtac aatcacaccc actgcagcta tttcatcaat gccatacaca ccaacagaat
     2341 caatcgaggc attaaagaat ttttacagaa catatggtag taatgtttgg ggtgagtatg
     2401 gatttaaaga tgcttttaat ccaagacaaa actggtttgc aagcagttat attgcaattg
     2461 atcaggggcc tataattgtt atgattgaaa attatagaag tggattgttg tgggaaaagt
     2521 ttatggctaa tcctgagata caaccaatgc ttgattcaat aggatttgtt cctgattctg
     2581 taacaagtgt tgatgaaaaa gttaaaactg ttaatgactt tgaattgctg ggcaattatc
     2641 ccaatccatt taatccttca acaactatcg tttttaatct taatgcgaat gaaaatgtca
     2701 aaattgaaat atttaatcat ttaggtgaaa gagttagcga agtttcgaat aaagaatttt
     2761 ccgctggtga aaataaagtt gtatggaacg gattaaatga tgaaaataaa aatgtaagtt
     2821 cagggatcta tctctacaaa attagcacgg cccaaagttc tctttatggt aaaatgattt
     2881 tacagaaata atgatgaggt tatcatttag aaaattaatt gtttttcttt tacctattgt
     2941 gatggtttta ttttcttgct caaaagaaaa cgaatcagaa aaaacaatta aattctgggc
     3001 aatgggcgct gaggctgaat atgttattaa acttgttcct gagtttgaga gattgaatcc
     3061 aggaattaaa gtaaaagttc agcaagtccc gtggacagca gcacaagaaa aattagttac
     3121 agcttttgca agtgataata ctcctgatgc ctgccagctt ggtaatacat gggtgccgca
     3181 gttttccgct ttaaatgcaa tcattccgct tgatgaattt attaaatcat ctaaccagat
     3241 aaacaaagaa aagtatttta cgggaatctg ggaaacaaat gtacttgatg atcaaatttt
     3301 tggaattccc tggtatatgg atacgcgtgt tatgttttac agaaaagatg tttttgaaaa
     3361 agcaggttac gaaaatccac ccaaaacatg ggcagaactt ttagacttat caataaaaat
     3421 aaaagctatg catccaaagg aagaaaagt
//
LOCUS       DASWBY010000213         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_573333, whole genome shotgun sequence.
ACCESSION   DASWBY010000213 DASWBY010000000
VERSION     DASWBY010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573333"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1128
                     /gene="carA"
                     /locus_tag="VF870_03910"
     CDS             52..1128
                     /gene="carA"
                     /locus_tag="VF870_03910"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX9251359.1"
                     /translation="MNEQKIKAKLILEDGSEFSGYSFGYEGNANGEVVFNTGMVGYPE
                     TMTDPSYRGQILVCTYPLIGNYGIPPKEMKDGLLKNFESNNIHCRALIVTDYSFEYSH
                     WSAERSLADWMVEEKIPGIYGIDTRMLTRKLRDKGTMLGKIVVDDKSDVGFLDPNKTN
                     LVGEVSVKDVVEYKAGNQKIVLVDCGTKNNIIRAFLGRDITVIRVPYDYDFTKIEANG
                     IVISNGPGDPKLNKKTIEHARIAMQSNKPILGICLGSQILALAAGADTYKLKYGHRGH
                     NQPCNELGTKRCYITSQNHGYAVDSKTLAQDWREWFVNDNDGTNEGIIHISKPFFASQ
                     FHPEASPGPDDSEFIFDMFVRALN"
     gene            1240..>2364
                     /locus_tag="VF870_03915"
     CDS             1240..>2364
                     /locus_tag="VF870_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9251360.1"
                     /translation="MIKKGKPKKVLILGSGALQIGQAGEFDYSGSQAIKALKEDGITT
                     ILVNPNIATIQTSENFADKVYFVPIKTEFVEKIILKEQPDSILLQFGGQTALNVGVDL
                     YDKGILEKYNIKVLGTPVDTIKDTEDRLLFSDRVAEVGLKVARSKTATTVDEAIKAAE
                     EINFPVMVRIAYALGGLGSGIVNNKEELTEKARRAFSFTNQILIEESLYGWKEVEYEI
                     VRDNYDNCITICSMENIDPMGIHTGDSVVIAPVQTLSAKENFKLRSIGIKLIRHLGII
                     GECNIQYALDPNSEDYRIIEVNARLSRSSALASKATGYPLAFIATKLALGYALNEVVN
                     IITQETSANFEPALDYVALKFPRWDLQKFQQVSTQLGSEMK"
BASE COUNT          809 a          411 c          474 g          670 t
ORIGIN      
        1 ttagccactt taggagtggc tattttttta tctgaaagaa catgatgaat tatgaatgaa
       61 caaaaaataa aagctaaact aatccttgaa gatggaagcg agttttccgg atacagtttt
      121 ggatatgaag gaaatgccaa cggcgaggtg gtatttaata ccggtatggt cggctacccc
      181 gaaacaatga ccgatccatc atatcgcgga caaattcttg tctgcactta tccgctaatt
      241 ggtaattatg gaattccacc aaaagaaatg aaggatggat tattaaaaaa ctttgaatcc
      301 aataatattc attgtcgtgc attgattgtt acggattatt cttttgaata ttcacattgg
      361 agtgccgaaa gatctttagc tgattggatg gttgaagaaa aaattcccgg tatttacgga
      421 attgatacta gaatgttaac aagaaaactc cgcgataaag ggacaatgct tggaaaaatt
      481 gtcgttgatg ataaatctga tgttggtttt ctggatccaa acaaaacaaa tcttgttggt
      541 gaagtaagtg ttaaagatgt tgttgagtac aaagccggta accaaaaaat cgttctggtc
      601 gattgcggaa caaagaataa tataattcgc gcttttcttg gaagagatat taccgtcatt
      661 cgtgttcctt atgattatga ctttacaaaa atagaagcaa atggaattgt aatatccaat
      721 ggtcccggcg atccgaaatt aaataaaaaa acaatagaac acgcaaggat tgcaatgcaa
      781 tcaaacaaac caattcttgg tatttgtctc ggatcgcaaa tacttgcact cgctgccgga
      841 gccgatactt acaaattaaa atatggccac cgcggacaca accagccttg taatgagcta
      901 ggaacaaagc gctgctatat tacttcacag aaccatggat atgctgtcga ttccaaaact
      961 ttagcacagg attggcgtga atggtttgtc aatgacaacg atggaaccaa tgagggaatt
     1021 attcatatat caaaaccatt ttttgcttcg cagtttcatc cggaagcttc tcccggaccg
     1081 gatgacagcg aatttatttt tgatatgttt gttagagcac taaattaaaa catccaaagt
     1141 catcctgagc gaagtcgaag gatgacatta ttaataaaag attttaatat agtcacactt
     1201 cgacttcgct cagtgtgaca gaaatactga attcagatta tgataaaaaa aggtaaacca
     1261 aaaaaagttt taattctcgg cagcggtgct ttgcaaatcg ggcaagctgg cgagtttgat
     1321 tactccggct cacaggcaat taaagcactt aaagaagatg gaattacaac tatacttgtc
     1381 aatccaaaca ttgcaacaat tcaaacatca gaaaactttg cggataaggt ttactttgtt
     1441 cctatcaaaa ctgagtttgt tgaaaaaata attctgaaag aacaacctga ttcaattctt
     1501 cttcagttcg gaggacaaac agcactgaac gtgggagtag atttatacga taaaggaatc
     1561 ttagaaaagt ataatattaa agttcttgga actcccgttg atacaattaa ggacactgaa
     1621 gatagactct tatttagtga tcgtgttgca gaagttggat taaaagtagc acggagcaaa
     1681 acggcaacaa ctgttgatga agcaattaaa gcggccgaag aaattaattt tcctgtaatg
     1741 gtgcgcattg cttatgctct gggtgggtta ggttccggaa ttgtaaacaa caaagaagag
     1801 ttaaccgaga aagcacgaag agcattttca ttcacaaatc aaattttaat tgaagaatct
     1861 ctttacggat ggaaagaagt cgagtacgaa attgtccgtg ataattatga taattgcatt
     1921 acgatctgct caatggaaaa tatagatccg atgggaatac acactggcga cagcgttgta
     1981 attgctcccg tgcaaactct ttctgcgaaa gaaaatttta aacttcgttc aatcggaata
     2041 aaattaattc gccatcttgg aattattggc gaatgcaata tccaatatgc acttgatcca
     2101 aattccgaag attacagaat tatagaagtt aacgcgcgat taagccgaag ctctgctctc
     2161 gcatcaaaag caactggcta tccgcttgca tttattgcga caaaacttgc acttggttat
     2221 gcacttaatg aagttgtaaa tattatcaca caggaaacct ctgcaaactt tgagcccgca
     2281 cttgattatg ttgcattaaa atttccgcga tgggatttac agaaatttca gcaggtaagc
     2341 actcagcttg gttctgaaat gaaa
//
LOCUS       DASWBY010000214         4205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_646586, whole genome shotgun sequence.
ACCESSION   DASWBY010000214 DASWBY010000000
VERSION     DASWBY010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646586"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..134
                     /locus_tag="VF870_03920"
     CDS             9..134
                     /locus_tag="VF870_03920"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HEX9251361.1"
                     /translation="MNGIEVYLCWRSDENDILYYHEMDAGYLGRKLIPPQYFDED"
     gene            complement(139..564)
                     /locus_tag="VF870_03925"
     CDS             complement(139..564)
                     /locus_tag="VF870_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251362.1"
                     /translation="MKKIFLFAICFLIIAACSKKDIKFEAISSEAFAFDIGDGTAEVN
                     STVRVKGFVQTEKDKIYNAKVAYEIDLLKPDSTVEKSIFNFVQKDEESEPISDIALDA
                     QFTLDSTYKAGTYTLIYKIADKNSEKTLEAKVNFDLTWD"
     gene            complement(654..1757)
                     /gene="serC"
                     /locus_tag="VF870_03930"
     CDS             complement(654..1757)
                     /gene="serC"
                     /locus_tag="VF870_03930"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX9251363.1"
                     /translation="MEKRIYNFSAGPAILPEEVLLEAQKELFMLPGVGMSILEISHRS
                     KTFDAILADAKAGIKQLLNVPDNYEILFLQGGASLQFSMVPLNLMPPKNKADYISTGS
                     WSKKAIKEAKRVGTVNVAATTEEGEGENKFFKRIPKQSELKLDADAAYVHFTSNNTIY
                     GTQWMSEPEVGNVPLVCDASSDILHKPIDVSKYALIYAGAQKNIGPSGVVLVIIRKDM
                     LERSQDSLHTMLNYKIQAENNSMYNTPNTFGIYIIKLVTKYLLGLGGLDKMYEINKAK
                     AKLLYDAIDSSNGFYKGHAEKDSRSLMNITFNLATPDLEKKLIDEATKAGFSGLKGHR
                     SVGGLRASIYNAFPIKGVEDLVAFMSDFQKRNS"
     gene            complement(1788..3035)
                     /locus_tag="VF870_03935"
     CDS             complement(1788..3035)
                     /locus_tag="VF870_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX9251364.1"
                     /translation="MAVIRPFKALRPTKEKSQQVASVPYDVVNLEEAKQFAAENPLSY
                     LHITRSEIDLPEVKDVYSKEVYLKAKENLNQIIEQAPLMVDEKAHFYLYRLIMDGRPQ
                     TGICATFSVDDYDNDVILKHEKTRKVKEDDRTNHIITTEAQTGVVFLTYRGVKTVNDL
                     VNKTMKDVAPEYDFTAPDGIQHTIWIMPDEYYQTIENEFAKIHKLYIADGHHRAKSAS
                     RAREEKMKSNPHHTGSEEYNYFIAVLFPEDQLKILPYNRVVFDLNGLSKADFMDSVAD
                     KFTITPTTDKEPKTKNTFCMYMDKEWFELKPRNSVFASLSLEKSVGEKLDVSILQNFL
                     LNPVLGIDDPRTNNRIDFIGGIRGTKELEKLVDSGKAAVAFSLYPVGLDDLMNISDAG
                     EVMPPKSTWFEPKLRDGLLTHLI"
     gene            3219..4013
                     /locus_tag="VF870_03940"
     CDS             3219..4013
                     /locus_tag="VF870_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9251365.1"
                     /translation="MAEEIKKTRRKPGQEKDEIVGKVEYHKDVFSPILNNKRDIVVWL
                     PIGYNSQKSSNKKYPVLYMHDGQNIMDPKTAYVGKDWRVDETVMKLIKQKKIKEIIVV
                     GIYNSPDRLDEYSWTEKGQNYLKFIVTELKPFIDSKYKTLSDKDNTAIMGSSMGGLIS
                     FYAAWHYPDVFSMAGCLSSSFYFNNDRSIKQVEEYTGHKKHVKFYIDHGEDGSTRGQR
                     MFVELSKKGYVIGQDIDYFYSPGAEHNEREWAARLERPLLFFFKSK"
BASE COUNT         1331 a          725 c          685 g         1464 t
ORIGIN      
        1 ctgcaatgat gaacggtata gaagtttatt tgtgctggag aagcgatgaa aatgatattt
       61 tatattatca cgaaatggat gccggatatt tgggcagaaa attaattccg ccccaatatt
      121 ttgatgaaga ctaaaatctt aatcccacgt taaatcaaaa ttaacttttg cttctaatgt
      181 cttctctgag tttttatcag caattttata gataagagtg tatgttcctg ctttgtaagt
      241 tgaatcaagt gtaaactgag catctagagc aatatctgaa attggttcgc tttcttcatc
      301 tttctgaaca aaattaaaaa tagatttttc tactgtacta tccggtttta gtaaatcaat
      361 ttcataagca acttttgcat tgtaaatttt atctttttcg gtttgtacaa aaccctttac
      421 tctaacagtt gaatttactt ctgcagtgcc gtctccaata tcaaatgcaa aagcttctga
      481 acttattgct tcaaatttaa tatctttttt actgcatgct gcaattatta aaaaacagat
      541 tgcaaataaa aatatttttt tcatcttatt ccttcaggtc attgcgaaaa gcgaagcgat
      601 gaagcaatct gctttattat agattgcttc gcttcacgcg caattacaaa taattaacta
      661 tttctttttt gaaaatcact cataaatgct actaaatcct caacgccttt tattgggaat
      721 gcattgtaga ttgatgcacg caatccacca actgaacgat gacctttcaa tccgctaaat
      781 cctgcttttg ttgcttcatc aataagcttc ttttccaaat cgggagttgc aagattaaaa
      841 gtaatattca ttaatgaacg ggaatctttt tcagcgtgac ctttataaaa tccattactg
      901 ctgtcaattg catcgtaaag aagttttgct tttgctttat tgatctcgta catcttatca
      961 agtccgccca agcccaataa gtattttgta actagtttaa ttatgtaaat accaaatgtg
     1021 tttggagtat tgtacataga attattttca gcctggatct tataatttaa cattgtatgt
     1081 aaagaatcct ggcttctttc aagcatgtct tttctgatta taacaagcac aactccagaa
     1141 ggaccaatgt tcttttgagc gcctgcatag atcaaggcat atttactcac atcaattggt
     1201 ttgtgaagaa tatctgatga agcatcacag acaagtggaa cgttaccaac ttccggttcg
     1261 ctcatccatt gagttccata aattgtattg tttgatgtaa agtgaacata agcagcatca
     1321 gcatcaagtt ttaattcact ctgtttggga attcgtttga aaaatttatt ttctccttca
     1381 ccttcttcag ttgttgccgc aacgtttaca gttccaacgc gttttgcttc tttgattgct
     1441 tttttagacc agcttcctgt ggagatataa tcagctttat ttttgggagg cattaagttt
     1501 aatggcacca ttgaaaattg taggcttgca ccaccctgca aaaataaaat ttcataatta
     1561 tcgggaacgt ttaataattg tttaatccct gctttcgcat cagcaagaat tgcatcaaat
     1621 gtctttgaac ggtgagaaat ttccaggata gacattccaa ctcccggtaa cataaacaat
     1681 tctttttgtg cttcaagtaa aacttcttct ggtaaaattg ccggaccagc gctaaaatta
     1741 taaattcttt tttccataat atttttcctt tagtaaattt tattttttta aattaagtgt
     1801 gttaacaatc catctctaag ttttggttca aaccaggttg atttgggcgg cattacttca
     1861 ccggcatcgg aaatattcat aaggtcatca agtccaacag gataaagtga aaatgcaacg
     1921 gcagctttac ctgaatcaac aagtttttct aattcttttg tgccgcgtat accaccaata
     1981 aaatctattc tgttatttgt tctcggatca tcaatgccaa gaacaggatt taataaaaag
     2041 ttttgcagaa tacttacatc aagtttttct cccacagatt tttctaaaga taaacttgca
     2101 aaaacagaat ttcttggttt aagttcgaac cattctttat ccatatacat acagaaagtg
     2161 ttttttgttt ttggctcttt atcggttgtt ggagtgattg taaacttatc tgcaactgaa
     2221 tccataaaat ctgctttgct taatccattt aaatcaaaaa caacacggtt ataaggaagt
     2281 atctttaatt gatcttccgg aaaaagtacg gcaataaaat aattgtactc ttcacttccg
     2341 gtatgatgtg gattagattt catcttttct tcacgtgcac ggcttgcact ttttgcgcga
     2401 tgatggccat cggcaatata tagtttatga atctttgcaa actcgttttc aattgtttga
     2461 taatattcat caggcataat ccaaatcgta tgctgaattc catccggagc agtaaaatca
     2521 tattcaggcg ctacatcttt catggtttta ttaacaagat cattaacagt cttaactcca
     2581 cgataagtta aaaacacaac acctgtttgt gcttctgtgg ttataatgtg atttgttcta
     2641 tcatcttctt ttactttacg agttttttcg tgttttagaa ttacatcatt atcataatca
     2701 tcaacagaaa aagttgcaca aatacctgtt tgcggtcttc catccataat taatctataa
     2761 agataaaaat gtgctttctc atcaaccatt aatggtgctt gttcaattat ctggttaaga
     2821 ttttcttttg ctttaagata aacttctttt gagtaaacat cttttacttc tggcaaatct
     2881 atttctgatc tggtaatgtg taaataactt agtggatttt ctgcagcaaa ttgctttgct
     2941 tcttctaggt taactacatc ataaggtaca cttgcaactt gctgggattt ttcttttgta
     3001 ggtctaagtg ctttaaaagg tctgataaca gccataaaac caatctccgt ttttagaaaa
     3061 gattattaaa attccgtact tcaaaaatcc acaaaataat cgtaataaac cagttcattt
     3121 aatggacttt aactacgtca aaatttattt taattcaaat tcggggagac tcaaagaaat
     3181 tttctatctt tacagccgtt taataatatt ttaaaaaaat ggctgaagaa ataaaaaaaa
     3241 cacgaagaaa acccggacaa gaaaaagatg aaattgttgg aaaagtagaa tatcataaag
     3301 atgttttttc accaattctt aacaacaaaa gggatattgt tgtttggctg ccaatcggat
     3361 ataattcaca aaaaagttca aataaaaaat atcctgtgct ttacatgcac gatggccaga
     3421 acattatgga tcccaaaaca gcttatgtgg gcaaagactg gcgtgttgat gagacagtta
     3481 tgaaacttat caagcagaaa aagattaaag aaattattgt tgtgggaatt tataattctc
     3541 cggacagact tgatgaatat agctggaccg aaaaaggaca aaattatttg aagtttatcg
     3601 taacagaact taaaccattt atcgattcca aatacaaaac attatcggat aaagataaca
     3661 ccgctattat gggttcttca atgggtggat tgatttcttt ttatgcggca tggcattacc
     3721 cagatgtttt ttctatggcg ggatgtctat caagttcatt ttactttaat aatgatagaa
     3781 gcattaagca agttgaagaa tacactgggc ataaaaagca cgtaaagttt tatattgatc
     3841 acggtgaaga cggttctaca cggggacagc gaatgtttgt tgagctctct aaaaaaggtt
     3901 atgtaatagg gcaggatatt gattatttct attcacccgg cgcagagcat aatgaaagag
     3961 aatgggctgc aagattggaa agaccgctcc tgtttttttt caaaagcaaa taagtttgaa
     4021 ttttcattgc gaatcccgat tagggagaag caatcggtta atgactttat tgttcaattg
     4081 ttttaactct tctcacccta agcgaagtcg aagggtgaat gatcttatat aataactaaa
     4141 atgaattcta tgaaatcatt tgttttaact ttcacacttg ctctaactct atggagttgt
     4201 tcttc
//
LOCUS       DASWBY010000215         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_270572, whole genome shotgun sequence.
ACCESSION   DASWBY010000215 DASWBY010000000
VERSION     DASWBY010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270572"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1596
                     /locus_tag="VF870_03945"
     CDS             <1..>1596
                     /locus_tag="VF870_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9251366.1"
                     /translation="SFVVLIALPLSLLITFIVMKIVGLSANLMSLGGLAISIGMIIDA
                     TIIQVENVQRHLSEESINKPKLLTVLKAVLEVRKPSIFGELIIALTFIPILSLEGIEG
                     KMFGPLAKTVAIALLSSMMLSIFVIPVLCSMFLKAQPEKESIIIKYAKNLYQPILEYS
                     MKKKFTVLGIAGIFLAIALFLSTKLGSEFIPIMDEGAFDMDVALPPGVSLSKAMEVNQ
                     LAAQKMKKFDELEIIVSRTGQTGIALDTRGVDKTGYVGVLKPRSEWKRDITRDELTNE
                     MREELESIPGIGFGFSQPIQCRIDEIVAGTRAQLIIKLFGDDIEVLKKQSDEIAKTLS
                     TIEGVTDLMTEKVSGQPYLTVNINRSEIARYGLNIREVQNVIEIAIAGKSASKLYEEN
                     RSFDITVRLPEEKRNSIETIENILIPTSNGANIPLGQLADVEMIEGPVQISREDGIRR
                     IGIEMNISGRDIGSFVEEAKQKIKNNIQLPAGYYLTWGGQFENQQRAMNRLMIIGPVV
                     IGLILLLLFVTFKSIRLALLVIS"
BASE COUNT          579 a          218 c          339 g          460 t
ORIGIN      
        1 ggagctttgt agttttaatt gcactaccac tttcgttatt aataactttt attgttatga
       61 aaatcgttgg attaagtgca aatcttatgt cacttggagg acttgcaata tcaataggaa
      121 tgataatcga tgcaacaata attcaggttg aaaatgtgca acggcatttg agtgaagaaa
      181 gcattaataa accaaagtta ttaacagttc taaaagcagt tttggaagta agaaaaccaa
      241 gcatatttgg agaattgata atcgcattaa catttattcc aattctatca ttagaaggta
      301 ttgaaggaaa aatgttcgga ccacttgcca agacagttgc aatcgcactg ctttcttcaa
      361 tgatgctttc aatatttgta ataccggttt tatgctcgat gttcctaaag gcacagccgg
      421 aaaaagaaag tataatcatt aagtatgcaa agaatttata tcagcccatt cttgaatatt
      481 cgatgaagaa aaaatttact gtattaggaa tagcagggat atttttggcg attgctctgt
      541 ttctatctac aaaacttggt tctgagttta tacctattat ggatgaaggt gcatttgata
      601 tggatgttgc tctacctccg ggagtatctt tatctaaagc aatggaagtc aatcagctag
      661 cagcacaaaa gatgaaaaaa tttgatgagc ttgaaataat cgtttcgaga acagggcaaa
      721 cgggtattgc tcttgataca aggggagttg ataaaaccgg ttacgttggt gttctaaaac
      781 caagaagtga gtggaagcgt gatattacga gagatgaatt aacaaatgag atgagggaag
      841 aactggaatc aataccagga attggatttg gatttagtca accgatacag tgcaggatag
      901 atgaaattgt agcgggtaca cgagcgcagt taattataaa actttttggt gatgatattg
      961 aagtacttaa aaaacaatcg gatgaaatag caaaaactct cagtacaatt gaaggagtaa
     1021 cagatctgat gacagaaaaa gtaagcggtc agccttattt aacagttaac attaacagat
     1081 cagaaatcgc aagatatgga ttgaatataa gggaagttca gaatgttata gaaattgcaa
     1141 ttgcaggaaa atctgcgtcg aaactttatg aagaaaatag aagctttgat ataaccgtac
     1201 gattgcctga agaaaaaaga aactcgatag aaacgattga aaatattctt attccaacaa
     1261 gtaatggtgc aaacatacca cttgggcaat tagctgatgt agaaatgatc gagggacctg
     1321 ttcaaatcag ccgtgaagac ggcatacgca gaataggtat tgaaatgaat ataagtggta
     1381 gagatattgg aagctttgta gaagaggcaa aacaaaaaat aaaaaataat attcaacttc
     1441 ctgcaggata ttatttaacg tggggtggac agtttgaaaa ccaacaaaga gcaatgaata
     1501 ggctgatgat aattggaccg gtcgttatag ggttaatctt acttttgtta tttgtaacat
     1561 ttaagtcaat aagattggct ttgcttgtaa tttcaa
//
LOCUS       DASWBY010000216         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_496283, whole genome shotgun sequence.
ACCESSION   DASWBY010000216 DASWBY010000000
VERSION     DASWBY010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496283"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..>2468)
                     /locus_tag="VF870_03950"
     CDS             complement(42..>2468)
                     /locus_tag="VF870_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251367.1"
                     /translation="EGFPVSNGNFKITNSMIMPETESRELETITGKFLFEITINGLLA
                     ASVASNLGLPDLFDTNTGLSAIGRFGLMDGQSIFAYNGCFPSEPSAWEKIDLGWANAV
                     EIAPGSYDINLVTKLAATLADTVILKVPLNSSEYYLIENRQRDVFNDGAAITYKSNGN
                     IITRTFLKDTTGFYSYDTDSLSGVVLDVDEFDWALPGNGIVVWHIDQNVIDEKLAENK
                     INTDKKRRGVDVEEADGVQDIGEQFTTIFGDVVIGEGTEQDFWYADNKAELFKDIFNK
                     DSRPDTKTNTGANSLITIKDFSSLANKMSFKVEYGDSIIKPLFSSNINGVLTESSSIT
                     LIPPSESTSNLPNFAILSASGQLFINDLDQTLYKNSNFSEHKVVSTSYGSRSYILGSN
                     GSKINIVYNAATYPDTGFIFQSVDLGYQITAPLVLRKTLTEQIEVLVGISSGVVNVYD
                     LQSILDNNPVVKDIFLAGRANDDVTQISNLEDEYSFITYNKFEQPEYLGQFTDKHGYF
                     IFNNEIPTQVASTKDKDNSYLSAVLTSSNNVYLIKGNEVISTFHIPADTAISSFALAD
                     LKNDGNNYIIVNNGNIQAYNFQGSLADNFSYSDPNGMRFIGSPIVADIEGDLKSEIIS
                     LTEDGRIFAIDGGSGKLVPGFPISLGISVYGSSLIFDYENKLVYSGLVPSGLLGWSVS
                     STTGRKDWSELFGNNSNHSSLSSASNTNTINEFFPTSRAYNYPNPVYNGQTAIRYFVG
                     EDSKINIKIFDLAGDFVAELNDDAQGGLDNETVWNVSNIQSGVYLARIEANGSSGKSE
                     SVIIKIAVVK"
BASE COUNT          814 a          453 c          385 g          816 t
ORIGIN      
        1 aataataatt tcataaatag attattgagg tgaaaaagaa attatttaac tacggctatt
       61 ttaattataa cggattctga tttgccgctg gatccatttg cttcgatgcg tgcaagataa
      121 acgccgcttt gaatattact tacattccaa acggtttcat tatccagtcc gccctgcgca
      181 tcatcattaa gttcagcgac aaaatcacca gccagatcaa atatcttaat atttattttg
      241 gaatcttcac caacaaaata tctaattgct gtttgtccgt tgtaaacagg attaggataa
      301 ttataagctc gtgaagttgg gaaaaattcg tttatcgtat ttgtatttga agctgaagat
      361 agtgaagaat gatttgaatt atttccgaat aattcagacc agtcttttct gccggtcgta
      421 gaagaaacag accatcccag caaacctgat ggaactaatc cggagtaaac taatttgttt
      481 tcataatcaa aaatcagaga tgaaccataa acgctgatcc ctaaagaaat gggaaaacct
      541 ggaacaagtt ttcctgatcc cccatcaatc gcaaaaattc gcccatcttc cgttaacgaa
      601 attatttcac tttttaagtc accttcaata tctgcaacta ttggcgatcc aataaatcgc
      661 attccgtttg gatcactata agagaaatta tctgctaatg aaccttgaaa attatatgct
      721 tggatattac cgttattaac gattatataa ttgtttccat catttttcaa atcagctaat
      781 gcgaaggatg aaattgctgt atctgctggg atatgaaatg tcgaaataac ttcattacct
      841 tttatcaaat aaacattatt acttgaagta agtacagctg aaaggtaaga gttatctttg
      901 tctttagttg aagctacttg agttggtatc tcattattaa atataaaata accgtgctta
      961 tcagtaaatt gtccaagata ctcaggttgc tcaaatttat tataagttat aaatgaatat
     1021 tcatcttcta aatttgatat ctgagtaaca tcatcgttcg cccttccggc taagaaaatg
     1081 tctttgacta cagggttatt atctaagatt gattgtaaat cataaacatt aacaacacca
     1141 cttgaaatgc cgaccaaaac ttcgatttgt tcagtcaaag tttttcgcaa gactaaaggt
     1201 gcagtaattt gatatccaag atccacagat tgaaaaataa atcctgtatc aggataagta
     1261 gctgcattat aaacaatgtt aattttagag ccattagatc ctaaaatata acttctggaa
     1321 ccataggaag tgcttacaac tttatgctct gaaaaatttg aatttttata cagtgtttga
     1381 tctagatcat tgatgaacaa ctgacctgaa gcggataaaa ttgcaaagtt tggtaaatta
     1441 cttgttgatt cggatggagg gatcagtgtt atagatgaag attcggttaa aacaccatta
     1501 atatttgaac taaacaatgg tttaattata gaatcaccgt actcaacctt aaaagacatt
     1561 ttgttggcaa gtgaagaaaa atctttaatt gtgattaaac tgtttgcacc tgtatttgtt
     1621 ttagtatcag gacgtgaatc tttgttaaaa atatctttaa ataattcagc tttattatcc
     1681 gcataccaaa aatcctgttc agttccctct ccaattacta catcaccaaa aattgttgta
     1741 aactgctctc caatatcctg cactccatca gcttcctcta catcaacgcc gcgtcttttt
     1801 ttatcggtgt taattttatt ttcagccaac ttttcatcta taacattttg gtcaatatgc
     1861 cagacaacaa ttccatttcc gggtaatgcc caatcaaatt catcaacatc aagaacaact
     1921 ccggataaag aatctgtatc ataactgtaa aaacctgtgg tatcttttaa gaaagtcctt
     1981 gtaataatat ttccatttga tttataggtg attgctgcac catcattaaa aacatctctt
     2041 tgtctgtttt cgataagata atattcagaa gaatttaatg gaactttcaa aataactgta
     2101 tcagctaaag ttgctgccag tttggtgaca aggtttatat cataactgcc gggcgcaatt
     2161 tcaacagcat ttgcccagcc aagatctatt ttttcccagg cggatggttc ggaaggaaaa
     2221 catccattgt acgcaaagat cgattgcccg tccattaatc caaatctgcc gattgcactc
     2281 aatccggtat ttgtatcaaa taaatctggc agaccaagat tacttgcaac gcttgcagct
     2341 aataaaccgt taatagtaat ttcaaataaa aatttacctg ttatcgtttc taattctctg
     2401 ctctcagtct caggcattat catgctattt gtaattttga aattaccgtt tgaaaccggg
     2461 aatccttc
//
LOCUS       DASWBY010000217         2847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_721604, whole genome shotgun sequence.
ACCESSION   DASWBY010000217 DASWBY010000000
VERSION     DASWBY010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721604"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VF870_03955"
     CDS             complement(<1..621)
                     /locus_tag="VF870_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251368.1"
                     /translation="MKKIKLPLLIMTLILFKCSSVQELTLSDEFINKINLFKENLDSL
                     KQEFKLPASTKVESAVIDSSDSTIIIEFNRNFSVVPFREGNVNEIYKFVKNSFGPRFE
                     NYKYTITSMGYPIENLIPNFYLTDKSKIDSSKLPKKKTERIPIVQNASKNLKLTKGLA
                     GRNILLWHSHGWYYNNKEKRWMWQRARLFQTVEDVGPMSFTIPYLIP"
     gene            complement(744..>2847)
                     /locus_tag="VF870_03960"
     CDS             complement(744..>2847)
                     /locus_tag="VF870_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEX9251369.1"
                     /translation="DIEPWSRWLTGSQGTPPSPFYDPLQFAIDEAHKRGMELHGWFNP
                     YRAERSVGNYPLSPTHVINQHPDWILQVGTVKFLNPGLQEVRDYVTSVFMDVARRYDV
                     DGIHIDDYFYVEGITNQDAATFAAYPRGFTNLGDWRRDNVNILIKEINDSLMSVKPWI
                     KWGVSPRGIWRNGVPPGIVGNDNYSTIYCDAMAWLHDRSIDYINPQLYWAFGGGQDYG
                     KLMPWWADSAGANERHMYVGHAVYRIGNPFNATEVPRQIRLNRTDIDCQGSVLYNTSS
                     TLANPLGFYDSLKTDLYRYPALPPIMNWKDIIAPNPIQNLRFERLASGQAGLTWDLPQ
                     IASDGDSAKRYVVYRFDYSNVQPSDLENSANIYNVEGSRESIPGEPPSPTGPYYFVVT
                     SLDRNFNESTMSNVLQVNPPPVPVLALPVNNAVNIEDTVILKWNYPALASSYGLQIST
                     DPTFASGIFLNQRGILDTFKVITGLEGQQQYYWRVNSTNAGGTSSFSNSYSFTTGFPS
                     TPLLAYPVNNTGNIPVDTTIYWYSTPAAVSYDLILARSADFAPNTIVVNAQGVTDTSI
                     IVTGMNVNTFHFWKVRANNTFGTSNWSAVWRFKTVNPLGIEDEELMPEEFVLEQNYPN
                     PFNPSTNFRFKISEAGLTTLKIYNLLGQEVAEVVNEFMSPGTYDIRFDAEGLASGIYF
                     YRIRVNEFSASKKMILVR"
BASE COUNT          906 a          551 c          479 g          911 t
ORIGIN      
        1 cgggatcagg taaggaattg taaaagacat tggtccaaca tcttcaactg tctggaataa
       61 tcttgcacgc tgccacatcc agcgtttctc tttgttattg tagtaccaac cgtgactgtg
      121 ccacaacaaa atatttcgtc ccgctaatcc ttttgtcagt ttcaaatttt tactggcatt
      181 ttgaacaatc ggaatccgtt cagttttttt ctttggaagt tttgatgaat caattttaga
      241 tttatcagtt aaatagaaat ttggtattaa attctcaatg gggtatccca tagaagtgat
      301 tgtatattta taattttcaa atcgtggacc aaaagaattt tttacaaatt tataaatctc
      361 atttacattt ccttctctaa aagggacaac tgaaaagttt ctgttaaact ctattataat
      421 cgtactatca gaactatcga taacagcact ttcaaccttt gtgcttgcag gaagtttaaa
      481 ctcttgcttt aatgaatcaa gattttcctt aaataaattt attttattta taaactcatc
      541 tgataaagtc agttcttgca cagatgaaca tttaaaaagg atgagagtca tgataaggag
      601 aggtaatttt atttttttca tatcacatca gtaattctaa gttgatagtt taaaatactt
      661 ttttacatta catatttaag atgaattgaa ttgattaaaa caaaaaaccc cttcaattaa
      721 agaaggggtg ttttgtcaat ttattatctt accaaaatca ttttcttaga tgccgaaaat
      781 tcattcactc tgattctata aaaataaatc ccacttgcca aaccttctgc atcaaatctt
      841 atatcgtatg tgccgggact cataaattca tttacaactt ctgcaacttc ctgtccaagc
      901 aagttataaa tttttaaagt tgtaagccct gcttcagaaa ttttaaatct aaaattagta
      961 gaaggattaa aaggatttgg ataattctgt tcaagcacaa attcttcagg catcaattct
     1021 tcatcctcaa ttcctaaagg attgactgtt ttaaatctcc aaacagctga ccaattactt
     1081 gttccgaaag tattattcgc tctcactttc caaaaatgaa aagtatttac attcattcca
     1141 gtaacaataa tacttgtatc ggtcactccc tgagcattaa ccacaattgt attcggtgcg
     1201 aagtcagcac tgcgggctaa tatcaagtca taagaaaccg ccgcaggagt ggagtaccaa
     1261 taaatagttg tatcaacagg aatatttccc gtattgttta caggataagc aagtaatgga
     1321 gtagatggaa atcctgtagt aaaactatat gaattggaaa aactgcttgt gcctccggca
     1381 ttggttgaat taaccctcca ataatattgc tgctgtcctt ccagacctgt aataacttta
     1441 aaggtatcta aaatacccct ttgatttagg aatattccag aagcaaaagt tggatcggta
     1501 gaaatttgta aaccgtacga ggatgcaagt gcaggataat tccacttaag aataactgta
     1561 tcctctatgt tcactgcatt attaacaggt aatgctaaaa caggaacagg aggaggatta
     1621 acctgaagca cattgctcat tgtgctttca ttaaaatttc tgtcgagaga ggtaaccaca
     1681 aaatagtaag ggcctgttgg tgatggcggt tcaccgggaa tactttcacg tgaaccttca
     1741 acgttataaa tatttgctga attttctaga tcacttggtt gaacgtttga ataatcaaat
     1801 ctgtaaacaa catatcgctt ggcgctgtca ccatcacttg cgatctgtgg aaggtcccat
     1861 gtgagtccag cttgaccgct tgctaatctt tcaaatctta aattttgaat cgggtttggt
     1921 gcaataatat ctttccagtt cattattgga ggtagtgccg gatatctgta taaatcagtt
     1981 ttaagtgaat cgtaaaatcc taagggatta gctaaagttg aagaagtatt ataaagaaca
     2041 ctcccctggc aatcaatatc tgtccggttt aaacgaattt gcctgggaac ttcagtagcg
     2101 ttaaaaggat taccaattct ataaacagca tggccaacat acatgtgcct ttcattagcg
     2161 ccagcagaat cagcccacca gggcataagt tttccataat cctgtccgcc accaaaagcc
     2221 caatatagct gtggattaat ataatcaata gacctgtcgt gcagccaggc catagcatca
     2281 cagtaaatcg tactataatt atcgtttcca actatcccgg gaggaacccc atttctccag
     2341 ataccacgtg gacttactcc ccacttaatc cagggtttaa ctgacattaa actatcatta
     2401 atttctttga tcaaaatatt tacattatct ctgcgccaat cacctaagtt tgtaaatcct
     2461 cttggatacg ctgcaaatgt agcagcatcc tgatttgtaa ttccttcaac ataaaagtaa
     2521 tcatcaatat gaattccatc tacatcatat cttcttgcta catccataaa aacagaagtt
     2581 acataatctc ttacttcctg gagaccggga ttaagaaact tgacagttcc aacctgtaga
     2641 atccagtctg gatgctggtt aataacatga gtgggagaca aaggataatt tcctaccgat
     2701 ctttctgctc tatatggatt aaaccatcca tgtaattcca tacctctctt atgtgcttca
     2761 tcaattgcaa attggagtgg gtcgtaaaaa ggacttggtg gtgtgccttg tgaaccggtt
     2821 agccagcgag accagggttc aatatcc
//
LOCUS       DASWBY010000218         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_9729, whole genome shotgun sequence.
ACCESSION   DASWBY010000218 DASWBY010000000
VERSION     DASWBY010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9729"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="VF870_03965"
     CDS             <1..1567
                     /locus_tag="VF870_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX9251370.1"
                     /translation="VFDNFISTNKLEHFAFDDVDNLEITQRFVDAKNFPEDIKNKLRE
                     FLKITNCPLAVRSSSLLEDSQFLPFAGVYETYMIPNSNPDIEERLEELLQSIKSVYAS
                     TFHKKAKDYIKSTSYGLEEEKMAVVIQRLVGAQREQRFYPDFAGVAKSYNFYPIAPQK
                     STDGIALVALGLGKTVVEGGNSIRFCPKYPKHMLQFFSPKETLKNAQQKFYALDLNSK
                     LDHHPDSIEDPLVKSFNLSDSEKDGTLSPVGSTYSAENDGIFDGISRNGIRVVTFAPI
                     LKHKIFPLPEILDLLLDFGSWGMGTPVEIEFAVNLSVPAGERKEFAMLQMRPMVLSRE
                     NEELTIGEIKDEDTICYSKQVLGNGAINGLYDIVVVDYDKFDRGKSKDVAIEVSTLNS
                     KLLAERKPYLLIGVGRWGSMDPWLGIPITWDQISGASVIVEAGFKDLSVTPSQGSHFF
                     QNLTSFRVGYFTVNRNDQPSFIDWNWLADQKAIEELNFTRHLRFDKPINVRMNGHESR
                     GVILKPGVDLFEI"
     gene            1557..1925
                     /locus_tag="VF870_03970"
     CDS             1557..1925
                     /locus_tag="VF870_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251371.1"
                     /translation="MKYKNLNLAFLIEIFVGLGTIISISLIGPKGLAALAILAIRPFV
                     LQKEEIRDKKNYFEFSYKILSNSLSIIFIMIISIIIILQFIPIWKTKLPPVEVMFVIL
                     IPFFLLTHGVIGIINLSTKE"
     gene            1927..3891
                     /locus_tag="VF870_03975"
     CDS             1927..3891
                     /locus_tag="VF870_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9251372.1"
                     /translation="MPDFLIIQDIVVILLVSLPIIFLFKKINLPSIVGFLIAGMIIGP
                     YGFSLIKSVNQISVMAEIGVILLMFTIGLEFSLSQLIRIKKFLLIAGGLQLLITIIIS
                     TLIFSALGIEINLAIFFSLLVSLSSTAIVLKILSDRDELESPQGKISIGILIFQDLAI
                     VPMFLLIPILSGIGELGAADIALKLLIAFGVIAGLLLLARFLMPLIVYQLANIRSREA
                     FTIGVILLLLGTAYITHSFGLSFALGAFIAGLILSESDYNHQIVSDILPFRDSFNSIF
                     FVSIGLLLNIQFVLENVLLISSLTLGILIIKTLVIVSIVYFMKYPLRVGILAGLGLAQ
                     IGEFSFVLAQAGLNFKLIADGYYNSFLASTIFSMILTPLLIKLSPFIAGRTSTLEKVT
                     KHSESYLENLHAHVIIAGFGLNGSNLARVLKETGIKYIVTELNPDTVKKEKAKGEKII
                     YGDISKEEVLKAIKIDKASIIVYAISDPAVTRMSLKLVKKINPHIYALVRTRYVNEVD
                     ELKKLGADDVIPEEFETALQIFRKVLERYHIPLNVIMKQTTLLREESYRFLRKEGEDF
                     TSFTHLDEILAQGLTETYYVNDDNVHLNKSLTEINLRAKTEATIIAIVRDGKTISNPS
                     AKEIIKVADTLVIYGTHLSVDKAIDLLDEK"
BASE COUNT         1401 a          668 c          764 g         1465 t
ORIGIN      
        1 agtttttgat aattttatca gcacaaataa actagaacat tttgcatttg atgatgttga
       61 taatttagaa attactcaaa ggttcgttga tgcaaagaat tttccggaag atataaaaaa
      121 caaattaaga gaatttctta aaataactaa ttgcccgctt gccgtaagat catcaagtct
      181 tttagaagat tcgcaatttc ttccttttgc aggagtctat gaaacttata tgattcccaa
      241 cagcaatcct gatattgagg aacgattaga ggaattatta caatctataa aaagtgttta
      301 tgcttctacg tttcataaaa aagcaaaaga ttatataaaa tctacttcat atggtttaga
      361 agaagaaaag atggcggttg ttattcaaag attagtcgga gcacaaagag aacaacggtt
      421 ttatcctgat tttgcagggg tggcaaagtc ttataatttt tacccgatag cgcctcaaaa
      481 atctactgac ggaattgcac ttgttgccct tggattaggt aagaccgttg ttgagggcgg
      541 caactcaata agattttgcc ctaaataccc caagcacatg cttcaatttt tttctccaaa
      601 ggaaacactt aaaaatgcac agcaaaaatt ttatgcatta gatttaaaca gtaaacttga
      661 tcatcatccg gatagcattg aagatccgct tgttaaaagt tttaatctat ccgactctga
      721 gaaagatgga acactttctc ctgtcggctc aacttactcg gctgaaaatg atggaatctt
      781 tgatggaatt tctcgcaatg gaattagagt tgttacattt gctccaatct taaaacataa
      841 aatttttcca ctacccgaaa tactggatct gttattggat tttggttctt ggggaatggg
      901 aacgcctgtt gaaatagagt ttgctgtaaa tctcagcgtt cccgcgggtg aacgaaaaga
      961 attcgcaatg cttcagatgc gcccaatggt tcttagtcgt gaaaatgaag aattgacgat
     1021 cggtgaaatc aaagatgaag atacgatttg ttacagcaaa caggtgttag gtaatggagc
     1081 aattaatggt ttatacgata tcgttgttgt tgactacgat aaatttgatc gtggtaaaag
     1141 taaagatgtt gccattgaag taagcacatt aaattcaaag cttttggctg aaagaaaacc
     1201 ttatttgtta attggtgtag gtagatgggg ctctatggat ccatggcttg gtataccaat
     1261 tacatgggat caaatttcag gagcatcagt gattgttgaa gccggcttta aagatttaag
     1321 tgtaactcca tctcaagggt ctcacttttt tcaaaacctt acttcattta gagtaggata
     1381 ttttactgtt aacaggaatg atcaaccaag ctttattgat tggaattggt tagctgatca
     1441 aaaagcaatt gaagaattaa attttacccg ccacttaagg tttgataaac ccattaatgt
     1501 tagaatgaat gggcacgaaa gtcgtggcgt aattttaaaa cccggagtcg atttatttga
     1561 aatataaaaa cttaaatctt gcttttttga ttgaaatatt tgtaggactt ggtactatta
     1621 tctctatcag tttaattggt ccgaaagggt tggcggctct ggcgatatta gctatccgtc
     1681 catttgtttt acagaaagaa gaaatccggg ataaaaaaaa ttatttcgaa ttttcatata
     1741 aaattttatc aaatagttta tcaataattt ttataatgat aatttcaata attattattc
     1801 tgcaattcat ccccatttgg aaaacaaaac ttcctcctgt ggaagtaatg tttgttatcc
     1861 tgattccttt ttttctgctt actcatggtg tgattggcat tataaattta tcaactaaag
     1921 agtagtatgc cggatttttt aattatacaa gatatcgttg taattcttct tgtatctctg
     1981 ccaatcattt ttctatttaa aaaaattaat cttccaagta tagtcggatt tttaattgcg
     2041 ggaatgataa ttggcccata tggatttagc ctaattaaat ccgtaaatca gattagcgtg
     2101 atggcagaaa ttggtgttat tcttttaatg tttactatcg gattagaatt ctccctgtct
     2161 cagttaatca gaataaaaaa atttttactt atcgccggtg gtttacaact tcttattaca
     2221 attattataa gcaccttaat tttttctgcg ttaggaatcg aaattaatct ggcaattttc
     2281 ttttcgctgc tggttagttt atccagcaca gcaatagttc ttaaaatact ttccgataga
     2341 gatgaacttg aatcgccaca aggtaaaata tctattggga tactaatctt tcaggatctt
     2401 gcaattgtac caatgtttct tttgattccg atattaagtg gaattggtga attgggcgct
     2461 gcagatattg ccttaaaact tcttattgcc tttggcgtaa ttgccgggtt acttttgctg
     2521 gctaggtttt taatgccgct gattgtatat caacttgcaa atataagatc acgagaagca
     2581 tttacaattg gtgttattct gttattgctc ggcactgctt atatcactca ttcatttgga
     2641 ttatcatttg cactcggtgc ttttattgcg ggattaatac tatcggaatc agattataat
     2701 catcaaatag tttctgatat tttaccattt cgcgattcat ttaacagcat ctttttcgtt
     2761 tcgatcggtt tgcttctaaa cattcaattt gtgttggaaa atgttttact tataagcagc
     2821 cttacacttg gaatactgat aataaaaaca ctcgttattg tttccatagt ctattttatg
     2881 aagtatccat tgcgtgttgg tattcttgcg ggattaggct tagcacaaat tggcgagttt
     2941 tcatttgtgc ttgcacaagc aggattaaac ttcaaactta ttgcagatgg ttattataat
     3001 agttttcttg cttctacaat tttttctatg attcttactc cattattaat taaactttct
     3061 ccttttattg ccggaagaac atctacatta gaaaaagtta cgaaacacag tgaaagttat
     3121 ttagaaaacc tccatgctca cgtaatcatt gcagggtttg gactgaatgg aagcaatctt
     3181 gcaagagtat taaaagaaac tggaattaaa tacattgtaa cagaacttaa tcccgataca
     3241 gtgaaaaagg aaaaagcaaa aggtgaaaaa ataatttatg gcgatataag taaagaagag
     3301 gttttaaagg ctatcaagat tgataaagca agtataatag tatatgctat ttctgatccc
     3361 gctgttacca ggatgtcttt aaagctagtt aaaaaaataa atcctcacat ttatgcttta
     3421 gtaaggacaa gatatgtaaa cgaagttgat gaattaaaaa aacttggtgc tgatgatgtg
     3481 attccggaag aatttgaaac agctctgcaa atctttagaa aagttttaga aagataccac
     3541 attcccctta acgtaattat gaagcaaact actcttcttc gtgaagaatc ttaccgtttc
     3601 ttaagaaaag aaggcgaaga ttttacttca ttcactcatc ttgatgaaat ccttgctcaa
     3661 ggtttaactg aaacttatta cgttaatgat gataacgttc atctcaataa aagtctaaca
     3721 gaaataaatc tacgtgcaaa aacggaagct acaataatcg caattgtgag agatggtaaa
     3781 acaatttcaa atccatctgc aaaagaaatt ataaaagttg cagatacact tgttatttat
     3841 ggtacgcatc tttctgttga taaggcaatt gatttgctgg atgaaaaata gataagctta
     3901 ttttttcctg cctcctttaa tcatcaaatc ttaccgatca ttaagaaaac ttataatgtg
     3961 tggtaacctt aaagtatttt tgagtgtcta atggatacaa tctttaggag tttattatga
     4021 aaatagttac aaagtattta atcgctttaa ctttaatctt caccacatag tttaatcaat
     4081 tatatgcttc atcaagtcat gatgacaaaa aagtaatcca cgaaaaaaca tttcaaatat
     4141 ctccgggtaa aaaattaaat gtttcagctg atggcggcga tattgtctgt tcggtgtggg
     4201 ataaatctga agtgtatatt aaagtaattg gcaccgatga tactgccgat aaatatgatt
     4261 ttacatttga ttcgaactcc gaagaagtaa ctgttaag
//
LOCUS       DASWBY010000219         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_721716, whole genome shotgun sequence.
ACCESSION   DASWBY010000219 DASWBY010000000
VERSION     DASWBY010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721716"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VF870_03980"
     CDS             complement(<1..912)
                     /locus_tag="VF870_03980"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9251373.1"
                     /translation="MKISLRWIKEFVDLNDISTDEIVTKLTMSGLEVEDVVDQTNLYK
                     NFIVGFVKETEKHPNADKLTICKVFDGKEDLQVICGAPNVQAGQKVVFAPIGTEIPNG
                     NFVIKKAKIRGVESNGMICAEDELLLSNDHSGIMVLDEKLEAGKPIIEALNLNDVVLE
                     IAITPNRPDALSHIGVARDLAALFDRELKIPEIKINESSDDVNKLVSVVIEDNVNCPR
                     YIAKVVKDVQIKESTQWLKNRLEKIGLRPRNNIVDITNLVLHECGQPLHAFDLDKLDN
                     NKIVVKSTKSETTYTTLDSKERKLPVNT"
     gene            complement(929..>1749)
                     /locus_tag="VF870_03985"
     CDS             complement(929..>1749)
                     /locus_tag="VF870_03985"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9251374.1"
                     /translation="RNEVSTYFEEVKERISSTQSSKENEIDLTLPGKEYIVGSKHILT
                     QTLDEIKSIFKGLGFSVANGPELESDYYNFESLNFPADHPARDMQDTFFVSKDFLLRT
                     HTTPVQIRIMEKQAPPVRAIMPGRVYRNEAVSARSYCMFHQVDGIYVDTDVTFAELKG
                     TLVSFAKQFYGSDLKYRFRPSFFPFTEPSAEMDITCYLCHGKGCKICKNSGWLEILGC
                     GMVDPNVFKFVDYDSEKYTGYAFGMGIERIALLKYGITDIRIFFDNDLRFLKQF"
BASE COUNT          555 a          350 c          238 g          606 t
ORIGIN      
        1 agtatttaca ggaagttttc gttctttaga atcaagagtt gtataagttg tttctgattt
       61 tgtactctta actacaattt tattattgtc aagtttatct aaatcgaatg cgtgtaatgg
      121 ttgtccacat tcgtgcaaaa ctagattagt aatatcaaca atattatttc ttggtcgcaa
      181 accaattttt tctaatctat tctttaacca ctgagtagat tcttttatct gaacatcttt
      241 tacaacttta gcaatgtaac gaggacaatt aacattatct tcaatcacta ctgaaactaa
      301 tttattcaca tcatcgctgg attcattaat ttttatttca ggaattttta actctctatc
      361 aaacaaagca gctaaatctc ttgcaacacc aatatgagat aatgcatcag gacgatttgg
      421 tgtaattgcg atttctaaaa ccacatcatt tagatttaat gcttcgataa ttggtttacc
      481 tgcttctagt ttttcatcca gcaccattat gcctgaatga tcattactca gcagcaactc
      541 gtcttccgca caaatcattc cgttggattc aactccgcga atcttagctt tcttaattac
      601 aaaatttcca tttggaattt cagtaccgat tggagcgaaa acaacttttt gtcctgcttg
      661 cacatttggg gcaccacaaa taacctgcaa atcttcttta ccatcaaata ctttgcagat
      721 agttaattta tcagcattag gatgcttttc agtttctttt acaaatccaa caataaaatt
      781 tttgtagaga ttagtttgat caacaacatc ttcaacttcc aatcccgaca tagtaagctt
      841 agtcacgatc tcatcggtgc ttatatcatt aagatcaaca aattctttta tccaacgaag
      901 tgaaattttc attttctttt tttccgcatt aaaactgttt taagaatctt aaatcattat
      961 caaagaaaat tcttatgtct gtaatcccat actttaacaa tgcaattctt tctattccca
     1021 taccaaaggc gtaaccagta tacttctcag aatcataatc aacaaactta aaaacatttg
     1081 gatcgaccat tccgcaacca agaatttcta accatccgga attcttacag atcttacagc
     1141 cttttccatg acacaaataa caagtgatat ccatttcagc actcggttct gtaaaaggga
     1201 aaaagctcgg acgaaaacga tatttcagat cggaaccata aaattgtttc gcaaaggaaa
     1261 ccaaagttcc tttcaactct gcaaatgtta catcagtatc aacataaatt ccatctacct
     1321 gatggaacat acaataactt cttgcactta ctgcttcatt tctataaact cgtccaggca
     1381 taatcgcgcg cacgggcgga gcttgttttt ccattatacg aatttgaact ggagtggtgt
     1441 gggtacgaag cagaaaatct ttgcttacaa aaaacgtatc ctgcatatct cttgccggat
     1501 gatctgccgg aaagtttaat gattcaaaat tataataatc agattccaac tcaggtccat
     1561 tagctacaga aaatcctaat cctttaaata ttgattttat ttcatcaagt gtttgagtaa
     1621 gaatatgttt acttccaaca atatattctt tgccgggtaa agtaaggtct atttcatttt
     1681 ctttacttga ctgagttgaa gaaattcttt cttttacttc ttcaaagtat gttgaaacct
     1741 cattcctaa
//
LOCUS       DASWBY010000220         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_383570, whole genome shotgun sequence.
ACCESSION   DASWBY010000220 DASWBY010000000
VERSION     DASWBY010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383570"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VF870_03990"
     CDS             <1..1023
                     /locus_tag="VF870_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251375.1"
                     /translation="WMETEPDQNQNGISGQKLSLDGTRQWTDNAKIFKDLSVAFTTSI
                     SYLTAEMGNGRAYLFYLEGNGSGLNDKVEGFACDTNGNFLWPGNFTILSNPTSDKMQM
                     VSTVDVYKNCKLVWGDNRLDGSGIYGQDINPDGQLGNSVIPVELTSFNANVSNSKVTL
                     NWITATELNNSGFEIERSCLTERNRNITDWESVGFVNGNGTSSQTHSYSFVDDNLLTG
                     KYSYRLKQIDFDGSFTYSKEVEVDLSVPQTFSLEQNYPNPFNPSTTIKYSIPNVTLTG
                     IEASRVILKVFDVLGNEVATLVNENKTAGNYEVNFDAKNLSNGAYFYKLQAGSFVETR
                     KMILLK"
     gene            complement(1063..1980)
                     /locus_tag="VF870_03995"
     CDS             complement(1063..1980)
                     /locus_tag="VF870_03995"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX9251376.1"
                     /translation="MFKQSQDPGLGEKYFKHTKRIINKDGSFNIKRTGGGFSSINAFH
                     FLINISWPKFLLIVFVGFVSVNLLFAILYQIAGIENLANAAGKDEMQSFLNTFFFSVQ
                     TFATVGYGGIHPSGIFSNIIASIESMTGILSFALATGLLYGRFSKPSAKIIFSDKAII
                     TTHKDGKALMFRVANSRPNVMMEMEANAMMTYLDKSNNQFTRKYFPIKLEIKFIYFFP
                     LPWTIVHQIDEDSPLWGKTENDLKELEAELLVMIKGFDDSFSQTVITRNSYKYNEIDW
                     NVKFVRAFSTDETGETIVDLEKLSSTEKI"
     gene            2158..3264
                     /locus_tag="VF870_04000"
     CDS             2158..3264
                     /locus_tag="VF870_04000"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX9251377.1"
                     /translation="MNQTNSAKKPEQKFRKTIREDFKSINFTKDFSNEYKSLSRFYLD
                     SEKKSQLESMTPIQRWVHQFAWLIKSMFLHLTPLRRILVLVGVLFLFTGRTISVNNGS
                     FSTNEGLFGGFLILFVLFLELKDKLLAKNELEEGRKVQQALTPEQNPKIAGWDIWLFT
                     RPANEVGGDLVDYLGLDENKTVLTIADVAGKGLQAALMTSKLQATIRALATEINSLSG
                     FGKKINKIFHRDSLPNLFASMVFIKIDSDSGKLNFINAGHFPPLIVRDNEIKELPKGG
                     IALGLVSNAEYNEQTIVLEQNEIFIAYSDGVFEAKNEFGQFFGMERFQKLIKNSFNNS
                     PQQIGKFIVSQLEHFIGDYPPTDDISLIIMKKKS"
BASE COUNT         1140 a          544 c          605 g         1092 t
ORIGIN      
        1 tggatggaaa ctgaaccgga tcaaaatcaa aatggtataa gcggacaaaa actttcattg
       61 gatggaacaa ggcaatggac agataatgca aaaatattta aagatttaag tgttgcattc
      121 accacttcaa taagttattt aactgctgag atgggaaatg gaagagctta tcttttctat
      181 cttgaaggaa atggatccgg gcttaatgat aaagtagagg gttttgcctg tgataccaat
      241 ggaaactttt tatggccggg caattttact attctttcaa atcctaccag cgataaaatg
      301 caaatggttt ctaccgtgga tgtttataaa aattgtaaac ttgtttgggg tgataatcgt
      361 ttagacggct ctggaattta tgggcaggat ataaatcctg atgggcagct gggtaattcg
      421 gttataccgg ttgaactcac atcatttaat gctaatgttt caaacagcaa ggttactcta
      481 aactggataa ccgcaacaga actcaacaac tcgggttttg agattgaacg cagttgtcta
      541 actgagcgaa atcgaaatat aacagattgg gaatcagtcg gttttgttaa tggaaacggt
      601 acatcttctc aaacgcattc atattctttt gttgatgata atttattaac aggaaaatat
      661 tcatacaggc ttaagcaaat cgattttgat ggttctttta cttactcaaa agaagttgaa
      721 gttgatttaa gcgtgccgca aacattttca cttgaacaga attatcccaa tccatttaat
      781 ccaagcacaa ctataaaata ttccattcct aatgtcaccc tgaccgggat cgaggcgtcc
      841 cgggtgattt tgaaagtttt tgatgttctt ggaaatgaag ttgccacatt ggttaacgaa
      901 aacaaaacag ccggcaatta tgaggttaat tttgatgcaa agaatctttc aaacggagcg
      961 tacttctaca aattacaagc gggatctttt gtagaaacaa gaaaaatgat tttgctaaaa
     1021 taagtctttc cattaaagga cggctcattt gccgtccttt aattatattt tttctgtgga
     1081 actcaacttc tcaagatcaa caattgtttc tcctgtttca tcagttgaaa aagcacgaac
     1141 aaattttaca ttccaatcga tttcattata cttataagaa tttcgcgtaa ttactgtttg
     1201 agaaaaacta tcatcaaatc ctttgatcat aactaacagc tctgcttcaa gctctttaag
     1261 gtcattttct gtttttcccc aaagtggact atcctcatca atttgatgta caattgtcca
     1321 gggcaacggg aaaaagtaaa taaatttaat ctcaagttta ataggaaaat attttctggt
     1381 aaactgatta ttgcttttat caagataggt catcatcgca tttgcttcca tttccatcat
     1441 aacgttggga cgggaatttg caacacgaaa cattaacgct tttccatctt tatgtgttgt
     1501 aataattgct ttatcactaa aaataatttt ggccgaaggt tttgaaaatc tgccataaag
     1561 caagccggtt gctaaagcaa aacttaatat tccggtcata gattctattg aagcaattat
     1621 gttagaaaaa atccctgatg ggtgaattcc tccgtaacca accgtggcaa aggtttgaac
     1681 actaaaaaag aaggtgttta agaatgactg catttcatct ttgccagcag cattagcaag
     1741 gttttcaata ccagcaatct gataaagtat tgcaaacaac aaattaactg acacaaatcc
     1801 aacaaaaact attaaaagaa atttgggcca gctaatattg attaaaaaat gaaaagcatt
     1861 aattgaagag aatccaccac cggttctttt aatattaaat gaaccatctt tattgataat
     1921 acgcttggtg tgtttgaaat atttttcacc aagtcccggg tcttgtgatt gcttaaacat
     1981 attatttgga ttagttttta attattgttt gtgaagatat aaaatgaaat tgacattttt
     2041 tgtgattttt aaattttata atcatctaat tcattgtcta agccgttcat caattaaccc
     2101 ttgtctctat tgagtgtttt atctaagttt tgatagaaat tattaattac agataaaatg
     2161 aatcaaacaa actctgcgaa aaaaccggaa caaaaattca gaaaaactat ccgggaagat
     2221 tttaagagca ttaattttac aaaagacttt agcaatgaat acaaaagctt aagccgtttc
     2281 tatctggatt cagaaaaaaa atcacaattg gaatcgatga ccccaataca aagatgggta
     2341 catcagtttg catggctgat aaaaagtatg tttcttcatt taactccatt acgtagaatt
     2401 ctcgttcttg ttggcgtact gtttttattt accggaagaa ctatttcagt taacaatgga
     2461 tctttttcaa ctaacgaagg gttgtttggc gggtttttaa ttttatttgt tttatttctt
     2521 gaacttaaag acaagttgct tgctaaaaac gaacttgaag aaggcagaaa agttcagcag
     2581 gcattaacgc ccgaacaaaa tccaaaaata gcaggatggg atatctggct gtttactcgc
     2641 cctgccaatg aagtgggcgg agatcttgta gattatttgg gattggatga aaacaaaact
     2701 gttttaacca ttgcggatgt tgcgggcaaa gggcttcagg ctgcacttat gacttcgaaa
     2761 ctacaggcaa caattcgtgc actagcaaca gaaattaatt ctctttcagg ttttggtaaa
     2821 aagattaata aaatttttca tcgcgatagt ttgcctaatt tatttgcatc aatggttttt
     2881 attaagattg attcagattc aggtaaacta aattttataa atgccgggca cttccctccg
     2941 cttattgtta gagacaatga aattaaagaa ctacccaagg gtggtattgc ccttgggctt
     3001 gtttcaaatg cagaatataa tgagcagaca attgtacttg aacaaaacga gatttttatt
     3061 gcatattcag acggagtgtt tgaagcaaaa aatgaatttg gtcagttttt tgggatggaa
     3121 agatttcaaa agctgataaa aaattcattt aataattctc cgcagcagat tggtaagttt
     3181 attgtttcgc agcttgagca cttcattggt gattatcctc ccacggatga catctctttg
     3241 attattatga aaaagaaatc ttaaaaaaga attttttctt gattttaatt aatgatttaa
     3301 gaatttttat ttaaaagagg caaaaaatta tgagcaaaac taaaaacacc ccaaaagaat
     3361 tatcctataa gcaatgtgaa g
//
LOCUS       DASWBY010000221         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_686920, whole genome shotgun sequence.
ACCESSION   DASWBY010000221 DASWBY010000000
VERSION     DASWBY010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686920"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VF870_04005"
     CDS             <1..881
                     /locus_tag="VF870_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX9251378.1"
                     /translation="YTIIIHGGAGFFPKDSPEELRKQYINSLTEALNIGKNILSTGGR
                     SIDAVEKVINYLENNKLFNSGKGAVFTNEGKHELDASIMQGKDLSSGAVAGVTIIKNP
                     ISLARLVMEKTEYVLFAGKGADDLGILLGVEIVPNSYFHDSTSYQKWLKSNLPKQQGE
                     TVGCVALDQYGDITAGTSTGGRQNKMPGRVGDSPLINAGTYANNKTCGVSATGVGELF
                     IRNTVAFNISALMEYKGYTLKEACDEMIYTILPAESGGIIALDKDGNYEMPFNTNSMM
                     RAVANSNGEFKVEIWK"
     gene            906..1412
                     /locus_tag="VF870_04010"
     CDS             906..1412
                     /locus_tag="VF870_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9251379.1"
                     /translation="MRPAKEDYAEYYQPYIDLVKGDDILRILVEQNMESQNILNSISE
                     SKGNYAYAEGKWTVKEVVGHMMDVERIFAYRALCIARGEIKPLPGMDQNEYVRNGGFN
                     KRQLFDLVYEYRLIRESNILLFGSFEKQVLLNRGNASGYDVTVLALMYMTAGHEKHHL
                     NVLLERYK"
BASE COUNT          532 a          225 c          327 g          476 t
ORIGIN      
        1 aatatacaat cattattcat ggtggagcag gtttttttcc aaaagattcg ccggaagaac
       61 tacggaagca atatattaac tcacttaccg aagctctgaa tattggaaaa aatattcttt
      121 ctacaggtgg aagaagcatt gatgcagttg aaaaagtaat taattattta gagaacaata
      181 aattatttaa ttcgggtaaa ggggcagtat ttacaaatga aggcaagcac gaacttgatg
      241 cttcaattat gcagggcaaa gatctttctt ccggtgcggt tgccggtgtt acaataatta
      301 aaaatccaat ttcacttgca cgattagtta tggaaaaaac agaatatgtt ctatttgccg
      361 gaaaaggcgc tgatgatctt ggaattttac ttggagttga aatagttcct aattcatatt
      421 ttcatgattc aacaagttat caaaaatggc tgaaatctaa tcttcccaaa cagcagggag
      481 aaactgttgg ttgcgttgct cttgatcaat atggcgatat aacagctggt acatcaacag
      541 gaggcagaca gaataaaatg cccgggagag tgggcgattc acctttaatc aatgcgggta
      601 cttatgctaa caacaaaact tgtggagttt ctgcaaccgg cgtcggtgaa ttatttatcc
      661 gaaacacagt agcttttaat atttcagctt tgatggaata caagggatat acattaaaag
      721 aagcttgcga tgaaatgatt tacacaatat tgcccgcaga aagcggtgga attattgcgt
      781 tggataaaga tggtaattat gaaatgccgt ttaatacaaa cagcatgatg agagccgttg
      841 ccaattctaa tggtgagttt aaagtagaaa tttggaagta aataaaaatt atatgtggag
      901 gttaaatgcg gcccgctaaa gaagattatg ctgaatatta ccaaccttat attgatctgg
      961 ttaagggtga tgatatttta agaattctgg tcgaacaaaa tatggaatct caaaacattt
     1021 taaattccat ttcagaaagc aaaggaaatt atgcttatgc agaaggaaag tggacagtaa
     1081 aagaagtggt cgggcatatg atggatgttg aaagaatttt tgcataccgt gcactctgta
     1141 ttgccagagg tgaaatcaaa cctttgcccg gaatggatca gaatgagtat gtgcgtaacg
     1201 gcgggtttaa caaaagacaa ttgtttgatt tagtgtatga atatcgttta atccgtgaat
     1261 ctaacatctt attatttgga agtttcgaaa aacaagtttt actgaacaga ggaaatgcaa
     1321 gcggctatga tgttacagtt ttagcattaa tgtatatgac tgccgggcat gaaaaacatc
     1381 atttgaatgt tttattggaa agatataagt agctatttaa atcactttat atattgtatc
     1441 aagaattttt tgcgttgctc caatatttga ttgaacataa gatgcagaaa tatttccaag
     1501 agtagtacgt aaattttcat tttcgaataa ccttctaaga tttcgatatg cctgccgttt
//
LOCUS       DASWBY010000222         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_123660, whole genome shotgun sequence.
ACCESSION   DASWBY010000222 DASWBY010000000
VERSION     DASWBY010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123660"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2360
                     /locus_tag="VF870_04015"
     CDS             <1..2360
                     /locus_tag="VF870_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9251380.1"
                     /translation="LELENSIIDDYKNYVDGLDELPEGLSFAAYEEWMKESVPELEVA
                     NSEIEKNERVIIPADIPLEVNSYVEQWITYFTGKGSGVMRRWLERSGKYFPMMTQVFD
                     EVGAPKQLVHLSMMESGLNPTARSWASAVGLWQFIKGTGKLYGLETSFYYDERRDPVK
                     STYAAARHLKDLYNSLGDWYLALAAYNCGEGRVTRAMRKAKDNSYWSVRPHLPKETRN
                     YVPIYIAVSIIAMDPEKYGFTDINYQKPYEYDTYTVDGAIDLNFLSSSANTDLATLQD
                     MNPELIQLSTPSNYSGGYPLKIPKGSMEIFAANLKNIPESAKRTFLVHTVRKGETVTK
                     IAKKYGISKNDLADANNITTKSKLYAGLQLKIPVLTSPSGTDFSDNTDTQIAQDQNSI
                     NSSNGYVSPYESLINDNSLIAEQQTDNSDEVIEETNEENVAVTEVENVIEESSTSIIP
                     EGTVAVSYRVKNDDSLLGIADLFNSRVSDIRNWNNIPYTTTIVVGQQLKIYVPEEMKD
                     YYTSLDKTTEIETNNNLVTTKNSKTSKNSGIVYHKIRRGETLGLIAAKYGVSVNQIRD
                     WNNISGSKIISGTNLRIYPDGTPNNNIAVNESNKNVTTKNNIYKYKVKRGDNLSEISE
                     KFGVSVHQIKKWNNISGDHIAAGKTLKIYNSTTSSSYGDNTTKNSANVNYYKIKPGDS
                     IGSIAEKYGVKISDIQKWNNISGNKILAGSTLKIYSDANVNDIESVAVNTSKNSKSNQ
                     QSYTVKSGDSLYSIASKNNTTVAKLKSLNNLTSNKIKAGQKIRLN"
BASE COUNT          889 a          340 c          428 g          704 t
ORIGIN      
        1 accttgaact cgaaaattca attattgatg actataaaaa ttatgtagat ggattagatg
       61 aacttcctga aggtttatca ttcgcggctt atgaagagtg gatgaaagaa tctgttccgg
      121 aacttgaggt tgctaattct gaaatcgaga aaaatgagag agttattatt cctgctgata
      181 ttcctttaga agttaattct tatgttgagc aatggataac ttattttacc ggtaagggca
      241 gcggtgtaat gagaagatgg ttagaaagat ccggcaagta ttttcctatg atgacgcaag
      301 tctttgatga agttggtgcc cctaaacaac ttgttcattt aagtatgatg gaaagcggtt
      361 taaatcctac agcaagatca tgggcaagcg ctgttggatt gtggcaattt attaaaggta
      421 caggaaaatt atatggtttg gaaacaagtt tttattatga tgaaagaaga gatcctgtaa
      481 aatcaaccta tgctgctgcc agacatttaa aagatctcta taatagttta ggtgattggt
      541 atcttgcatt ggccgcatac aattgtggtg aaggaagagt aacgagggca atgagaaaag
      601 caaaagataa ttcttactgg tctgttagac ctcatttgcc aaaagaaacc agaaattatg
      661 taccgattta tattgctgtt agtattatag caatggatcc ggagaaatat ggatttacag
      721 atattaacta tcaaaaacca tatgaatatg atacttacac ggttgatggt gcaattgatt
      781 tgaattttct ttcttcaagt gctaacactg atttagctac tctacaggat atgaatcctg
      841 agttaattca gctttctaca ccgtcaaatt actctggtgg gtatccttta aagataccaa
      901 aaggttctat ggaaatattt gcagcgaatc ttaaaaatat acctgagtca gctaaaagaa
      961 cttttttagt acatactgtt agaaaaggtg aaactgtaac taaaatcgca aaaaaatatg
     1021 ggatctctaa aaatgattta gcagatgcta ataacattac tacaaaatca aagctttatg
     1081 ctggtttaca attaaaaatt ccggttttaa ccagtccttc aggaactgat ttctcggata
     1141 atacagacac acaaattgca caggatcaaa attctataaa tagcagtaat ggttatgttt
     1201 ctccatatga aagtcttatt aacgataatt ctttaattgc agaacagcaa actgataatt
     1261 ctgacgaagt tattgaagaa acaaatgaag aaaacgtagc tgttactgaa gttgaaaacg
     1321 tgattgaaga atcttcgaca tcaataattc cggaaggaac cgttgcagtt tcttatcgtg
     1381 ttaaaaatga tgatagtttg ttaggtatag cagatctttt taattcaaga gttagtgaca
     1441 taagaaactg gaacaatatt ccttacacta caacaatcgt ggtcggtcag caattgaaaa
     1501 tttatgttcc agaagaaatg aaagattatt atacttcttt agataaaaca accgagattg
     1561 agacaaataa caatttagtg acgactaaga attcaaaaac atctaagaat tcaggaatcg
     1621 tttatcataa gattagaaga ggtgaaacac ttggtttaat cgcagctaaa tatggtgtta
     1681 gtgtaaatca gataagagat tggaataata tctcaggcag caaaataatt tccggtacaa
     1741 atctgcgtat ctatccagat ggaactccga acaacaatat tgccgttaac gaatcgaata
     1801 aaaatgttac aacaaaaaac aatatttata aatacaaagt taaaagaggc gataatttaa
     1861 gtgagatatc tgaaaaattt ggagtatctg ttcatcaaat taaaaaatgg aataatattt
     1921 ccggtgatca catagctgcc ggcaaaactt taaaaattta caatagtaca acaagttcat
     1981 catatggcga taacactact aagaattcag caaatgtgaa ttattacaaa attaaaccag
     2041 gcgattccat tggcagtatt gcagaaaaat acggagtaaa aatttctgat atccaaaaat
     2101 ggaataatat ttccggtaat aaaatactgg ctggcagcac tttaaaaatt tattctgatg
     2161 caaatgtaaa tgatattgag agtgtagctg taaatacaag taaaaattca aaatcgaatc
     2221 aacaaagtta tactgtaaaa agcggtgatt ctctctattc aattgcatca aagaataaca
     2281 caactgttgc taaattaaaa tcactaaata atcttactag caacaaaatt aaagcagggc
     2341 aaaagattag acttaattaa t
//
LOCUS       DASWBY010000223         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_534288, whole genome shotgun sequence.
ACCESSION   DASWBY010000223 DASWBY010000000
VERSION     DASWBY010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534288"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1305)
                     /locus_tag="VF870_04020"
     CDS             complement(52..1305)
                     /locus_tag="VF870_04020"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9251381.1"
                     /translation="MKPMKNERKNVIIIGAGFGGLSAAKLLDDEHLNIIVIDKTNHHL
                     FQPLLYQVATAALSPADIAIPIRSILNNKKNTKVILDEAIDIDKSSRIVQLKNSRLNF
                     DYLIVAPGSHHSYFGKDHWANFAPGLKTLYDALTIREKIINSLEMAEKEDDQNNKSKY
                     LTFVIVGGGPTGVELAGAIAEIAKKTIIKDYKNFKPDDVKVFLIEAYPRLLNTFVEKI
                     SLKAQNYLTSMGVQVVNDTRVEDVNDNGVKTSTGFIKTSNIIWAAGNQASSFLQKLDV
                     STDKSGRILVHNDCSIPEHPEIFVIGDAAQFEDDNGKILPGTAPVAMQQGRFVARIIN
                     AELNGKPRTKFKYIDKGNMATIGKAKAVAEIRGFKFYGLFAWLMWSSIHIFYLIGFKN
                     RFRVMIEWIWYYISNKRGTRLIVGK"
     gene            1482..>2202
                     /locus_tag="VF870_04025"
     CDS             1482..>2202
                     /locus_tag="VF870_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9251382.1"
                     /translation="MQSSKIDFLGKYNELLQTAIQSSADFPNKLCDFIIKEFELEATA
                     IFKVSNGSNLELIGKSNTVKKSLSRDVQATCPHCKNIIDNNQLNEFTIQANCDINITD
                     HILNEGCILLNITDGQTALVKFAKKTAFTKSEIDNIELIGNSVKNLLKLWIGSKGILN
                     NSLSNIISNIAQELRTPTNSIMGFASLLNEENLSPYQTEYVGTLKENAFELLSIINDL
                     IDLSKLESGITKVNLTSVNFKD"
BASE COUNT          745 a          360 c          332 g          765 t
ORIGIN      
        1 ttattttttg aaaactaaat taaggtaaat gcttaatcaa ggctaaacta attatttacc
       61 cacgattaat cttgttcccc ttttatttga tatatagtac caaatccatt caatcattac
      121 tctaaatcta tttttgaatc caattaaata aaaaatatga attgatgacc acatcagcca
      181 ggcaaacaaa ccataaaatt taaatcctct aatttcagca acagcttttg ccttaccgat
      241 ggtagccata ttacctttat caatatattt aaatttggtt cttggcttac catttaattc
      301 tgcgtttata attcttgcca caaatcttcc ctgctgcatc gctaccggtg cagtgccggg
      361 caaaattttt ccattatcat cttcaaattg tgcagcatca ccaataacaa aaatttccgg
      421 gtgttcagga attgagcaat cattatgcac aagtatcctt ccggatttgt cagtagaaac
      481 atcaagtttt tgcagaaatg acgatgcctg attacctgca gcccagatta tatttgaagt
      541 tttaataaat ccggtagaag ttttaactcc attatcgttt acatcttcca ctcttgtatc
      601 attaaccacc tgaacgccca ttgaggttaa atagttttgt gctttcagcg aaattttttc
      661 aacaaaagta tttaacaatc tgggataagc ttctattaag aatactttta catcatcggg
      721 cttaaaattt ttgtaatctt ttattatagt ttttttagca atctctgcaa tcgcacctgc
      781 taattcaact cctgtcgggc ctccgccaac aataacaaag gtaagatatt ttgatttatt
      841 attctgatca tcttcttttt ctgccatttc aagcgaatta attatctttt cccgaattgt
      901 taaagcatcg tacaaagttt ttaatccggg tgcaaagttt gcccaatgat ctttaccaaa
      961 atacgaatga tgggaaccgg gtgccacaat aagataatcg aaattcagtc tggagttttt
     1021 taattgaaca attctacttg atttatcaat atctattgct tcatcaagta ttacttttgt
     1081 atttttttta ttgtttaata ttgatcgaat cgggatggca atatctgcag gtgaaagtgc
     1141 cgcggttgct acctgataaa gaagtggctg aaaaagatga tgatttgttt tgtcaataac
     1201 aattatattt aaatgttcat catctaaaag ttttgcggcg gataatcccc caaatccggc
     1261 tccgattata attacatttt ttcgttcgtt tttcataggt ttcattatta aaatattaaa
     1321 ttttagggct gattaatgat catgctaaaa agcgattatt gataaaatag taataaatat
     1381 tttagaataa tacttcagga ttgtttaatt ttggattgaa gaattttcta tttagtttaa
     1441 ttgtagccta gaagttaatt tttaagaatc gaaaaaggaa tatgcaaagt tcaaaaatag
     1501 attttcttgg taaatacaac gagttgcttc aaactgccat tcaatcatca gccgattttc
     1561 caaataagtt gtgcgatttt ataattaaag aatttgagtt ggaagcaacg gcaattttta
     1621 aggttagtaa tggcagcaac cttgaattaa taggtaaatc taatactgta aaaaagagcc
     1681 tttccagaga tgtacaagca acatgcccgc attgtaaaaa tattattgat aacaatcagt
     1741 taaatgaatt tacaattcaa gctaattgtg atattaatat cacggatcat attttaaatg
     1801 agggatgtat tctattaaat attaccgacg gacaaactgc actggttaaa tttgctaaaa
     1861 aaacagcatt cacaaaaagt gaaatagata atattgagct gataggcaat agtgtaaaga
     1921 accttttaaa attatggatt ggtagtaaag gaattttaaa taactcactt agcaacataa
     1981 tttctaacat tgctcaggaa ctgcggacac ctactaacag cattatggga tttgcttctc
     2041 ttttaaatga agaaaatctt tctccttacc agacagaata tgtaggaact ttaaaagaaa
     2101 atgcatttga acttctatct attataaatg atctgattga tctaagtaaa cttgaaagtg
     2161 gaattacaaa agtaaacctc acatcagtta actttaaaga tt
//
LOCUS       DASWBY010000224         3410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_496434, whole genome shotgun sequence.
ACCESSION   DASWBY010000224 DASWBY010000000
VERSION     DASWBY010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496434"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VF870_04030"
     CDS             <1..714
                     /locus_tag="VF870_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9251383.1"
                     /translation="FLLSFFLSVAIMTTNYSQQEKQTSHSFQKEVKINLSANYLLYLP
                     KDYDDSDKPFPLVLFLHGMGERGTDIEKVKTHGIPKLINEGKEFPFIVVSPQCPDEYF
                     WNDDVLIALLDEIESNYVVDKNRIYVTGLSMGGHGTWTLALAQPNRFAAIAPVCGWGH
                     PVEFCKLAHMPIWVFHGAKDFVVPLSLSEQIVEQLKAFGSTKVNFTIYPEANHDSWTE
                     TYNNEELYKWFLEQSLDNK"
     gene            730..927
                     /locus_tag="VF870_04035"
     CDS             730..927
                     /locus_tag="VF870_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251384.1"
                     /translation="MYIKLLDKIKLVFKNDHKTEKEKIIISQKSCPHCASRGLFIFND
                     TPKISTAINEAELKDKNQNEN"
     gene            917..2182
                     /locus_tag="VF870_04040"
     CDS             917..2182
                     /locus_tag="VF870_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEX9251385.1"
                     /translation="MKINKIDLLLIKPHFDKLSVTLVVLLSVILSLNIFSQENNFVTV
                     NGKEIVAPDGKPILLKGINLGNWLNPEGYMFKFKNVNSFRLIDNTIKELIGADEAGKL
                     WKTFRDNYITKEDISFIKSTGLNHIRVPFNFKLFLVEDHPEIWLDEGFKRLDDVIKWC
                     KEENLYVLLDLHAAPGGQTGDNIDDSWSYPFLFENEQAQQTTIALWEKLAERYKDETI
                     VIGYDLLNEPIPHYMENKEDLNLLLEPLYKKITTAIREVDNNHIIFIGGAQWNTNFKM
                     FGKPFDNKLVYTFHKYWMPPVQEEIKDYVDFSNKYNVPMYLGESGENEDEWIDKFRVL
                     LENNNIGWCFWPYKKMDSNRGMVQFPKTLEWEEIIKYAETSKNNFEEIRNTKPGKETI
                     QKAFSDLLQNIKFKNCIINNGYLKALGIK"
     gene            2213..>3410
                     /locus_tag="VF870_04045"
     CDS             2213..>3410
                     /locus_tag="VF870_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251386.1"
                     /translation="MKILLNIVIALLLLNSFLLFPQSESSTVAQIGTEKITSQEFKLR
                     FELSPYIPSDKNIDPDSIKYDFLYSLIAEKLWAKEAEDLGVTNIDKFNFVFKPLEDMF
                     MRNALFKIEVEDKVFISADDIANGIIKSQTKLNAQIITSNDSLSIFNLYQKLNANSNF
                     DSLISKTENITNNVFDITLGSLKDEEIEDSIYSLPVNGYTDPIKSEVGWAVFIVKNKI
                     STSFDLGNQQTIDNMKKVIRNRRIEKRYKEYLNELLSGITINISPESFFLLSSSIWNV
                     LKNETNINNSKKYFELSEFDFNKISLALGPDNLNKQMFSLKEKQISIEDFLSTLAFNG
                     FHVLKLDSVIVRQKLNQEAKRFVEQQLITEEAYKRELQLTPQVRNDLQIWKENYLAQL
                     YFNNNLD"
BASE COUNT         1263 a          494 c          571 g         1082 t
ORIGIN      
        1 tttctactat catttttttt atcagttgca ataatgacaa caaattattc ccagcaagaa
       61 aaacaaactt cacatagctt tcaaaaagaa gttaaaatta atttatctgc aaattacctt
      121 ctatatctac caaaagatta cgatgatagt gataaaccct ttccattagt tctttttctg
      181 catggcatgg gggaaagagg aacagatatt gagaaagtaa aaacgcatgg gattccaaaa
      241 ttaataaatg aaggaaaaga attccctttc atcgtggttt cgcctcagtg tcctgatgaa
      301 tatttttgga atgatgatgt gctgatagca ttattggatg aaattgagag taattatgtt
      361 gtagataaaa ataggattta tgtaacagga ttaagtatgg gcggtcatgg aacatggaca
      421 ctcgcattgg cacagcccaa tcgttttgct gctattgcac ctgtttgcgg ctggggtcat
      481 cccgtcgaat tctgtaagtt agcgcatatg ccaatctggg tttttcacgg ggcaaaagat
      541 ttcgtggtac cattaagtct ttcagaacaa attgtagaac aattaaaagc ttttggttct
      601 accaaagtta attttacaat ctaccctgaa gcaaaccatg atagctggac agaaacttat
      661 aataacgaag aactttacaa atggtttctt gagcaatccc tagataataa ataaaatgaa
      721 ttgtgaaaaa tgtatataaa gttacttgat aagataaaat tagtttttaa gaatgatcat
      781 aagactgaaa aagagaaaat tataatctca caaaaatcct gtccgcattg tgccagccgg
      841 ggattgttta tttttaatga tacacccaaa atttcaacag caattaatga agcagaatta
      901 aaagataaaa atcaaaatga aaattaataa aattgatctg ttattaatta agccacactt
      961 cgacaagctc agtgtgacat tggttgtttt actttccgtt atactatctt taaatatttt
     1021 ctcacaagaa aataattttg taacggtaaa tggaaaagaa attgtagctc ccgatgggaa
     1081 accaatttta ctaaaaggaa tcaatcttgg taattggctt aatcccgaag gttatatgtt
     1141 caagttcaag aatgttaatt ctttcagatt aatcgataat acaataaagg aattaattgg
     1201 agcagatgaa gcaggaaaat tgtggaaaac atttagagac aattacataa caaaagaaga
     1261 tatcagtttt atcaaatcta caggtttgaa tcatatccgt gttccgttca actttaaatt
     1321 atttttagta gaagatcatc ctgaaatctg gcttgatgaa ggctttaaaa gattggatga
     1381 tgttatcaaa tggtgcaaag aagaaaatct ttatgtattg cttgatcttc atgcagcacc
     1441 tggcggacaa acaggtgata atattgatga cagctggagt tatccattct tatttgaaaa
     1501 tgaacaagct cagcaaacaa ctattgcact atgggaaaaa cttgccgagc gttataaaga
     1561 cgaaactatt gtaattggtt atgatttatt gaacgaacct attccccatt acatggaaaa
     1621 caaagaagac ttaaatcttc ttctggaacc actttataaa aaaatcacga cagctataag
     1681 agaagttgat aataatcata taatttttat cggtggcgcg caatggaaca caaactttaa
     1741 aatgtttggc aaaccattcg ataataaatt agtttatact tttcataaat actggatgcc
     1801 gcctgttcag gaagaaatta aggattatgt tgatttctcc aataaatata atgttccaat
     1861 gtatctaggg gaaagcggtg aaaatgaaga tgaatggatt gataagttca gagttttgct
     1921 agaaaataat aatattggat ggtgtttttg gccttataaa aaaatggatt caaaccgcgg
     1981 tatggttcaa ttccctaaaa cattagagtg ggaagaaatt ataaaatatg cggaaacttc
     2041 aaagaataat tttgaagaaa taaggaatac aaaaccaggc aaagaaacaa tacaaaaggc
     2101 attttcagat ctgcttcaaa atatcaaatt taaaaattgt attattaaca atggctactt
     2161 aaaagcattg ggaatcaaat agatttaatc cagaaaagat tacatttcac ctatgaaaat
     2221 attactaaat atagttatcg cattgctgtt actaaacagt tttttattgt ttccgcaaag
     2281 tgaatcatca accgttgcac agattggcac tgaaaagatt acatcgcagg aatttaaatt
     2341 acgatttgaa ctatcgcctt atattccgtc tgataaaaac atcgatccgg attcaattaa
     2401 atatgacttt ctttattcgt tgattgcaga aaaattgtgg gctaaagaag ctgaagatct
     2461 tggagttaca aacatagata aatttaattt tgtcttcaag ccgctggaag atatgtttat
     2521 gcggaatgct ctttttaaaa ttgaagtcga agataaagtt tttatttccg ctgatgatat
     2581 tgcgaatggg attattaaat cacaaacaaa acttaacgct caaatcatta cgtcaaatga
     2641 ttctttaagc attttcaatc tttatcaaaa attaaatgct aatagtaatt ttgattctct
     2701 catttcaaaa actgaaaata ttacaaataa tgtttttgac ataacattgg gatcactaaa
     2761 agatgaagag attgaagatt cgatttattc acttcctgta aatggctata ctgatccaat
     2821 taaaagtgag gttggctggg cagtctttat tgttaaaaat aaaatatcaa cttcctttga
     2881 tcttgggaat caacaaacta tagataatat gaaaaaagtg attcgcaata gaagaattga
     2941 aaaaagatat aaagaatatc ttaatgaatt actttcgggt atcactataa atattagtcc
     3001 tgaatcattt tttcttctaa gttctagcat ttggaatgtt ctaaagaatg aaactaatat
     3061 taataattcc aaaaaatatt tcgaactctc ggaatttgat tttaacaaaa tatcacttgc
     3121 tcttggacct gataatctta ataaacaaat gttttcttta aaagagaaac aaattagtat
     3181 tgaagatttt cttagcactt tagctttcaa tggatttcat gtacttaaac tcgattcggt
     3241 aatcgtacgt caaaaactta atcaggaagc taagcgattt gttgaacagc aattgattac
     3301 agaagaagct tataaacgcg aattacaact cacaccccag gttcgtaatg atttgcagat
     3361 atggaaagaa aattacctgg ctcaattata ttttaataat aaccttgatt
//
LOCUS       DASWBY010000225        11604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_270756, whole genome shotgun sequence.
ACCESSION   DASWBY010000225 DASWBY010000000
VERSION     DASWBY010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 11604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11604
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270756"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1832
                     /locus_tag="VF870_04050"
     CDS             <1..1832
                     /locus_tag="VF870_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251387.1"
                     /translation="LYNSSIGSKYFHIGNNTNIHYDDPATIPASGIDLLANIASGPYN
                     LNRGDTLVFYTAIVAGETYDELIVAANVAQFAMDNNFDLAKPPSRPTLYSSAGEFKAN
                     LYWDDSAEQSFDKLAGYDFEGYRLYKSTDRGINWKKIADYDILDNVGNNAGLQYSFTD
                     TNLVNGIEYWYSITAYDKGDSSFESLESAIGNTLAAINTVSVIPRSEAIGRTPISPED
                     VKNLNTGSSNYILNASSIDDETLAGNEYKTTFSFVPRKEFGDLATRTTVTVTDTAATK
                     PYKYAIEFTSPSTFDLKNITLDEVIREGYGYPFGGRDLLITGHGLRVSMRDSAGTPDD
                     FRPEKGDVIAINYSMTTVRNNLDSVINKRAYQLGQVQTSTDGVSLQLVEPEIIKSVSR
                     IGGTDNVEMTFEVADASLVKEKIYIASIDGNGIIDNNGFVLLSVSETDIQLDTLYSLD
                     TFTFDGIAGQITFPTNSPPAAGNKFSLETIKPVQPNIKDSYSFKIKGAQVDPQTVASE
                     LNKIRVVPNPYLVSSIYEPEFGELRREPLRQIQFVNLPPECTIYIFTVDADLIKTLYH
                     NATNGTETWDLRTEGGRELAAGMYIYVVKTKDSEFKERFAIIK"
     gene            1851..2843
                     /locus_tag="VF870_04055"
     CDS             1851..2843
                     /locus_tag="VF870_04055"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9251388.1"
                     /translation="MKKILFFLIVIGFGNIWAQNPNLGTSGAQFLQLPVGARSEAMGG
                     AIVGLADDASAIFWNPAGIVKVNNVQAHFSYMNWFDLFNFNAASLVYNAGDIGTFGAS
                     MVLFTTDDMEITTEEQPNGTGRYFDAGDVALGITYAKYLTDRFSVGLSVKYINQTIWN
                     ETASGVAFDIGTQYRLDFQNLTIAMSMTNFGADMRFDGPDLNFTYRQDEDYPQSRLVP
                     SRLLTQDYPLPLNFQVGIGFDVFEYDFVKMIGAIDVTHPNDNAERAHFGTEFSFFDRF
                     FLRAGYKYNYDDQDFTFGAGANVPLGSSAVYFDYAYSLYDILPSVHRISVNLSF"
     gene            2845..4062
                     /locus_tag="VF870_04060"
     CDS             2845..4062
                     /locus_tag="VF870_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251389.1"
                     /translation="MTKNIIFGLLTFLISFSVVKPCTTAVVSGKATDDGRPLLLKNRD
                     ADVLQNKLVYFFDGRFKFIGLVNSTDKQNSEVWCGFNEAGFGIMNSASYNLKINDTTR
                     LSDLEGRIMKMALGQCSTLADFEKLLNELPKPLGVEANFGVIDANGGAAYYETDNFKF
                     IKYDVNDPEVAPNGYLIRTNFSFSGEDNKGYGYIRYKTAEEIFQKQYNHGKIDFEFLI
                     NEVPRCLVHSFTKTDLVKNLPDESDNKYVFFRDYIPRHSTSAAIVVQGVKGNESPSLT
                     TMWTILGFPLTSVVIPVWLLEDGAMLKVLQGDETGNAPFCDLALQLKDKVFSDQNDAS
                     ENYLNLSALMNKENTGIRQELIPIEEQVILKGKNILNNFRKNGFKDSEAKDFYNWIDN
                     NVYKEITSKFKIN"
     gene            4153..5646
                     /locus_tag="VF870_04065"
     CDS             4153..5646
                     /locus_tag="VF870_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9251390.1"
                     /translation="MTKNILWLLILSISNLLSQNIQTPLEESNFSELTSHEELKHFVQ
                     KVDENYDLIKTEVLTKSSEGRELFVVYFSKAEFGKDESKIKVLIFAQQHGNEQSGKEG
                     ALLLIKELLKPENRFLFDKIDFALVPQMNPDGSEKNQRRNGNGKDLNRNHLILTEPET
                     IALHELFDNYHFEVTMDVHEYWPFGEEWKKLGFRKNYDETIGAITNINISKELRELSY
                     KEYIPFIFKYISNKGFTAFHYLPGGPPNVDYIRYSTFDINDGRQSFGIQNTFSFIQEG
                     INGENYSTDNLEHRAKGQMTGMLGLLEFAYNHKDEIKNLVATEREKLIGNQVSNKVAI
                     QLDHFSDGNKLELPQLSYYSNKDTMVTVDDFRPIVKSIYDVNRPKGYLIPKTLTEIIN
                     WADRQELQYSDYRMEVDDRVEQYTIVGFDSMDFERDTIINPVVEVAEVENNYCGDDYI
                     FIPVKQLKNNLIVIALEPKSELGLVTYKQFEHLLKKGENFPILRVVN"
     gene            5849..6988
                     /locus_tag="VF870_04070"
     CDS             5849..6988
                     /locus_tag="VF870_04070"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEX9251391.1"
                     /translation="MNVMVLGCGLIGRPMAIDLAKDKSFNVTVADVSKQNLDRISSEL
                     QIKKIQQDLSDLDQLKYLLKDIDIVLNAVPGFMGFNTLKEIIKAKKNVVDIAFFSEDP
                     FELDELAKQNNVTAVIDCGVAPGMSNLLSGYVNSILDKTETILIYVGGLPVVREYPYE
                     YKAGFSPIDVIEEYTRPARYIENGKLITRPALSDPEFINFDEIGTLEAFNSDGLRTLS
                     QTLNVPNMKEKTLRYPGHIGKMLVLRESGFFGSEEIEVTGVKIRPVDFTSKLLFPMWE
                     LKDGDKEFTVMKIIIDGVKNNKKIRYTYNLLDRHYEKTNTHSMARTTGYTATTVLRLL
                     AKGLFDRKGICPPEYIGQKSECVDFILNELKDRGVIYNLVIEKLG"
     gene            6994..8007
                     /locus_tag="VF870_04075"
     CDS             6994..8007
                     /locus_tag="VF870_04075"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX9251392.1"
                     /translation="MNHPLNKIPTITLANYPTPLDEANRLASKIGLTKLLIKRDDLTG
                     LAGGGNKARKLEFDFAEIINGGYDVVLTAGGIQSNHARMTAAAARKLGFDMKLVLGGP
                     DFEIVKGNLLLDILFNSEIRYLVDDDTNSSLESEMQLWADELLSEGRKPFIIPIGGST
                     GLGALGYVKAMEELSHQFENDDVQIVVGVGSCGTFAGTILGANIFLPKARVIGISVSR
                     NSEAIRQRTLELIEESAKLINFNIEKQISIECYDDYFIEYGMITEKGRQAIDLCANLE
                     GILLDPIYTGKVMAGLIDLKEKGVIDRKIPVVFIHTGGLPIIFSFEKEFGNKLNCTKI
                     FPQ"
     gene            8015..9517
                     /locus_tag="VF870_04080"
     CDS             8015..9517
                     /locus_tag="VF870_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9251393.1"
                     /translation="MVAKVLIIIICFIYFSPHSFTQDEKSFEAKEVKSLIEKFFNEDP
                     WVNGEAIGKLVKIGEPAVDDLIISLQDKYENVRWCSAITLEKISPLGRRSIPFLINAL
                     TDESKNVRWCSALALGKFKSEALKSIPALQKLLYDDDYDVRWAAYVAILKIDKKSLNV
                     IPDLLEKINIVEKLTPELMSQLKVPGVSISIIHNNNIAYSKSFGVADADTKLPVDINT
                     MFEACSMSKPVFTLLVLQLVDQGKLDLDSPLSDYLPETFVSDDDYSKLITARMILSHT
                     GGMPNWRKGDEERNAPIPIYFKPGTRFNYSGEGIYYLQRVVEKITNQSLENYARENLF
                     DKLGLTSTSFIWKENFDKQIATGHNADGKCNERKKYVHSNAAYTLYTTADEYAKIIVE
                     MTNPKIENGYSLSKNMADEMLTHQTRVDTREVIDRSGRNFGLFAFRGLGWAIDSTITG
                     DVVYHSGANQTGFRCYSQFSPKDKSGIVIMTNGENGNELWRRLIKEIGDL"
     gene            9633..10736
                     /gene="menC"
                     /locus_tag="VF870_04085"
     CDS             9633..10736
                     /gene="menC"
                     /locus_tag="VF870_04085"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX9251394.1"
                     /translation="MKIEKIELRHIKMELVSPFTTSMGTEYDEEHIIVRVDGEGYTGW
                     GESVAEGTPFYSYETVTTAWHILQDFLIPAILGKDISGIDSAIASYEKVRGHMMAKAG
                     LEAALWDLFAKSKNISLSKMMGGTRNKIDVGVSIGIQSTPEVLVKKIEGYLAEGYKRI
                     KIKIAPGNDIQFVKAVRKEFPDILFQVDANSAYELKHIDLLKQMDNLNLLLIEQPLGY
                     EDIYDHSKLQKDLKTPICLDESIHSLDDTRAAIELDSCRVINIKPGRVGGFTESILIH
                     DYCASKNIPVWCGGMLESGIGRAGNVALASLPNFTLPGDISASKRYYKEDIVEPEFVV
                     NKDGTMDVPTKPGIGVEVNMKMLEKVTVKKREF"
     gene            complement(10733..11212)
                     /locus_tag="VF870_04090"
     CDS             complement(10733..11212)
                     /locus_tag="VF870_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9251395.1"
                     /translation="MKFSTQEEYGLRLLLRIGKSDSDKGMTIPELSEQEKLSEANVGK
                     ILRALRLAGFIESSRGQTGGYKLASTPNEILVGDVLTALGGKLYESTFCDLHAGVENI
                     CTHSIDCSLRSLWKTVQTMLDGLLSKITLQDLLGNEQQVELFVTNITEELENSLPKS"
     gene            complement(11368..>11604)
                     /locus_tag="VF870_04095"
     CDS             complement(11368..>11604)
                     /locus_tag="VF870_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEX9251396.1"
                     /translation="IKGFPPSSPSIALFKNGELVHFVPRLNIEGYSAEQIATNLKNTF
                     NEICSAKGPSITPEQFAEVQYAKQCGSKIPLFKG"
BASE COUNT         3969 a         1783 c         2242 g         3610 t
ORIGIN      
        1 atctttacaa ttcatctata ggaagtaaat attttcatat aggcaataac actaacatcc
       61 attatgatga tccggctact atccccgcat caggtataga tttacttgct aatatcgcct
      121 ccgggcctta caacttaaat cgcggagata ccttggtttt ttacacggca atagttgcag
      181 gtgaaactta tgatgaattg attgttgcag ctaatgtagc ccaatttgca atggataata
      241 attttgatct tgctaaaccg cctagtcgtc cgactctgta ttcttcggct ggagaattta
      301 aagcaaattt atattgggat gacagcgctg aacaaagttt tgataaactt gctggttatg
      361 attttgaggg atatagactt tataaaagta ccgacagggg aatcaactgg aaaaaaatag
      421 ctgactatga tatactagat aatgttggaa acaatgccgg gctgcaatat agttttacag
      481 atacaaatct ggttaatgga attgagtact ggtattcaat aacagcatac gataaaggcg
      541 attcatcttt tgaaagttta gaaagtgcaa tcggaaatac acttgctgca attaatacag
      601 tatctgtaat accaagatca gaagcaattg gaagaacacc aatttcgccc gaagatgtaa
      661 agaatttaaa tacaggcagt tccaattata tacttaatgc ttcctcaata gatgatgaga
      721 ctcttgcagg caatgaatat aaaaccactt tcagttttgt cccaagaaaa gaatttggcg
      781 atttagccac tcgaactacc gttacggtta ctgatacagc agcgacaaag ccatataaat
      841 atgcaatcga atttacttca ccttcaacat tcgatttgaa aaacataaca cttgatgaag
      901 ttatcaggga aggatacggt tatccgtttg gtggaagaga tcttcttatc acaggtcatg
      961 gtcttcgtgt cagcatgaga gattcagccg gaaccccgga tgatttcaga ccagaaaagg
     1021 gagatgtaat tgcaataaat tattcgatga caaccgttag aaataattta gattcagtaa
     1081 taaataaaag agcttatcaa cttggtcagg tacaaacatc aacagacggt gtttcacttc
     1141 agttagttga accggagata atcaaatcag tatcaagaat aggcgggact gataatgtag
     1201 aaatgacttt tgaagttgcg gatgcaagtc ttgtgaaaga aaaaatctac atcgcatcca
     1261 ttgatggaaa tggaattatt gataataatg gtttcgtttt attatctgta tctgaaacgg
     1321 atatacaact tgacacatta tattcattag atactttcac atttgatgga attgcaggac
     1381 agattacatt cccgacaaac tctccgccag cagcaggtaa taaattttct ttggagacaa
     1441 ttaaaccagt acagcctaat attaaagatt catattcttt taaaattaaa ggcgcacagg
     1501 tagatcctca aacagttgca agcgagttaa ataaaattag agttgttcca aatccatatt
     1561 tggtttcttc aatttatgaa ccagagtttg gtgagttgag aagggaacca ttaaggcaaa
     1621 tccaatttgt aaatcttcct cctgaatgca caatatacat ctttactgtt gatgcagatc
     1681 tgataaaaac tctttatcat aatgcaacca atggaacaga aacctgggat ctaagaaccg
     1741 agggtggaag ggaattagct gccggaatgt acatctatgt tgtaaagact aaagattccg
     1801 agttcaaaga acgatttgca ataatcaaat aagctgatgc aggagattat atgaaaaaaa
     1861 tattattttt tttaatcgtt ataggctttg gaaatatttg ggcacaaaat ccaaatcttg
     1921 gaacttccgg agcccaattt cttcaattgc cggttggtgc ccgatcagaa gcaatgggcg
     1981 gcgctattgt tggacttgct gatgatgctt ctgcaatttt ctggaatcct gccggtattg
     2041 taaaagttaa taacgtacag gcacattttt cttatatgaa ttggtttgat ctttttaatt
     2101 tcaatgctgc ctcactggtc tataacgcag gcgatatcgg cacatttggt gcgagtatgg
     2161 tgctctttac aactgatgat atggagataa caacagaaga acagccaaat ggaaccggaa
     2221 gatattttga tgccggcgat gttgctctcg gaattactta tgctaaatac ttaaccgata
     2281 gattcagtgt tggtttaagt gtaaagtata ttaatcaaac tatctggaac gaaacagcat
     2341 cgggcgttgc atttgatatt ggaacacagt atcgattgga tttccaaaac cttacaattg
     2401 ctatgtctat gacaaacttt ggtgctgaca tgaggtttga tggaccggac cttaatttta
     2461 cttatcgaca agatgaagat tatccacaaa gcagattggt gccaagcaga ttacttaccc
     2521 aggactaccc gcttccatta aactttcaag tgggaatcgg ttttgatgta tttgaatatg
     2581 attttgtaaa aatgattggt gctattgatg taactcatcc taatgataat gccgaacgtg
     2641 cacactttgg aactgagttt tctttctttg atagattctt tcttcgcgcc ggatataaat
     2701 ataattatga tgatcaggac tttacctttg gggcaggggc aaacgttcca ctgggaagct
     2761 cagcagtata ttttgattat gcatattcat tgtatgatat tcttccaagc gtgcatcgta
     2821 tttctgtaaa tctgagtttt taaaatgact aaaaatataa tctttggtct gcttactttt
     2881 ttgatttcat tcagtgtggt taaaccatgc acaactgctg tggtttccgg taaagcaacc
     2941 gacgatggga gaccgctttt attaaaaaat cgtgatgcag atgttcttca aaataaactt
     3001 gtttatttct ttgatggacg atttaaattt attggattag taaactcaac agataaacaa
     3061 aattcagaag tatggtgcgg atttaatgaa gccggatttg ggataatgaa ttcagcttct
     3121 tacaatctaa agataaatga tacaacaagg ctgtctgatc ttgaaggcag aataatgaag
     3181 atggctcttg gacagtgttc aactcttgcg gattttgaaa aattattaaa tgaacttcca
     3241 aaaccgttgg gagttgaagc aaactttgga gttatcgatg caaacggcgg tgcagcatat
     3301 tatgaaactg ataattttaa atttattaag tatgatgtta atgatccgga agtagcacca
     3361 aacggttacc tgataagaac taacttttca ttttccgggg aagacaataa agggtacggt
     3421 tatataaggt ataaaaccgc ggaagaaatt tttcaaaagc agtataatca tggaaaaata
     3481 gattttgaat ttttgataaa cgaagtacca cgttgtttag tacactcatt tacaaaaact
     3541 gatcttgtaa aaaatcttcc agacgaatca gataataagt acgttttttt tagagattat
     3601 attccaagac actcaacatc tgcagctata gttgtgcaag gtgtcaaagg aaacgaatca
     3661 ccatcattaa ctacaatgtg gactatactc ggatttccac tcacttccgt tgtaatccct
     3721 gtttggcttc ttgaagatgg agcaatgctg aaagtactgc agggtgatga aacagggaat
     3781 gcaccatttt gtgatcttgc attacaattg aaagataaag ttttttcaga tcaaaatgat
     3841 gcaagtgaaa attatctaaa cctttccgct ttgatgaaca aagagaatac agggatcaga
     3901 caggaattaa ttccaattga agaacaggtt atattaaaag gaaaaaatat cttaaataat
     3961 tttagaaaaa acggtttcaa agattctgaa gcaaaagatt tttataattg gattgataac
     4021 aatgtttata aagaaattac atcaaagttt aagatcaact gatgctttca caaattagtt
     4081 ttcaaacaat agctgccttt ttgatttttc atcacaccat tatccacaaa caaaatcaat
     4141 ggtattatta aaatgactaa aaatattctc tggttactga ttttatcaat ctcaaatctt
     4201 ttatcacaaa atattcaaac tcctcttgaa gaatcaaact tcagcgaact tacttctcat
     4261 gaagagctta aacattttgt tcaaaaagtt gatgagaact atgatttgat caaaactgaa
     4321 gtgcttacaa aatcatccga gggaagagaa ttattcgtcg tctatttttc aaaagctgag
     4381 ttcgggaaag atgagagtaa aataaaagtc ctgatctttg ctcagcagca cggtaatgag
     4441 caatccggta aagaaggtgc tttactttta attaaagaat tactcaaacc ggaaaatcga
     4501 tttctatttg ataagattga ttttgcactt gttccgcaga tgaatccgga cggatctgaa
     4561 aagaatcagc gccggaatgg aaatggaaag gatttaaatc gaaatcattt aattctgacc
     4621 gaacctgaaa cgattgcact tcacgaatta ttcgataact atcattttga agtaacaatg
     4681 gatgtacacg agtattggcc ctttggagaa gaatggaaaa agttaggatt cagaaaaaac
     4741 tatgatgaaa caataggcgc aataactaat attaatattt ctaaagaatt acgtgagtta
     4801 tcatataaag aatatatacc ttttattttt aaatatattt cgaataaagg atttaccgca
     4861 tttcattact tgccaggagg tcctccaaat gttgattata ttcgttacag tacatttgat
     4921 ataaacgatg ggagacaaag ctttggaatc cagaatacat tttcatttat acaggaaggt
     4981 ataaatggtg aaaattacag cacggataat cttgaacatc gggcaaaagg acaaatgaca
     5041 ggaatgcttg gattacttga gtttgcatat aatcataaag atgaaataaa aaatttagtt
     5101 gcaactgaaa gagaaaagtt gattggcaat caagtttcaa ataaagttgc aattcaactc
     5161 gatcattttt cagatggaaa caaacttgaa cttccccagc tttcatatta ttcaaacaaa
     5221 gatactatgg tgacagtcga tgattttcgg ccaatagtaa aatcaattta tgatgttaat
     5281 agaccaaagg gttatttaat tccaaagact ttaactgaaa tcattaattg ggctgatagg
     5341 caagagcttc aatattctga ttatagaatg gaagttgatg atagggttga gcaatatact
     5401 atagttggat ttgattcaat ggattttgaa cgggatacta ttattaatcc tgtggttgag
     5461 gttgctgaag ttgaaaataa ttattgcggt gatgattaca tttttattcc ggtaaagcaa
     5521 cttaaaaata atttaattgt aattgctctt gagcctaaat cggaacttgg attagttact
     5581 tacaaacagt ttgaacatct tcttaagaaa ggtgaaaatt ttcctatact tagagtggta
     5641 aactagtttt attatagagt cattgcgagt gattaactac aatgaattcg aatgatgcaa
     5701 tctgtaaata ttatgcaatc atcagaacaa aatatcagta atcattcagt taattttatg
     5761 aatcgattta gtggactata aaatttcttt ctctaattaa ttaacgcaac tatgttgtaa
     5821 atacaaataa tttattcgga gaagaaaaat gaatgtaatg gttttaggtt gtggtcttat
     5881 tggccgtcct atggcaatag atctcgcaaa agataaatca tttaatgtaa ctgttgctga
     5941 tgtcagtaaa caaaatcttg atagaatttc ttcagaactt caaatcaaaa aaatccaaca
     6001 agatttatca gatcttgatc aattaaaata cttgcttaaa gatatagata ttgtgttaaa
     6061 tgcggttcca ggttttatgg gattcaatac gcttaaagaa atcatcaaag cgaaaaagaa
     6121 tgtggttgat atagccttct tttctgaaga tccatttgaa cttgatgaac tggcaaagca
     6181 aaataatgta acagcagtga tcgattgcgg cgttgcaccc ggaatgagca atttgctctc
     6241 aggatatgtt aattctatac tcgataaaac agaaacaatt ttaatttatg tcggtggttt
     6301 gccggttgtt cgggaatatc catatgaata caaagctgga ttttctccaa ttgatgtgat
     6361 tgaagaatat acccggcctg cgagatatat tgaaaatgga aaactgatta ctcgtcccgc
     6421 actttcagat cctgaattta taaactttga tgaaatagga acacttgagg catttaacag
     6481 cgatggactg cgtacattat cacaaacatt aaatgttcca aatatgaagg agaaaacact
     6541 tcgctatccg ggccatattg gaaaaatgct tgtgcttcga gagtcagggt tttttggtag
     6601 tgaagaaatt gaggtgactg gagtaaaaat tagacccgtt gattttactt ctaagttatt
     6661 gtttccaatg tgggaactaa aagacgggga taaagaattt accgttatga aaatcattat
     6721 tgatggtgta aagaataata aaaaaatcag atacacatat aatttattgg atcggcacta
     6781 tgagaaaaca aatacgcatt caatggcaag aactaccggt tacaccgcta caaccgtgtt
     6841 aagattatta gccaaaggat tatttgatag aaagggaatt tgtccgcctg aatatattgg
     6901 gcagaaatcc gaatgtgttg attttatttt gaatgaatta aaggaccgtg gagtaattta
     6961 taatctggta atagaaaaat taggttgata aaattgaatc acccactgaa taaaatacca
     7021 acaataacac ttgctaatta tccaacccct ttagatgaag ctaatcgatt agcaagtaaa
     7081 attggattaa caaaattgct tattaaacga gatgatttaa cgggtcttgc aggaggcgga
     7141 aataaagcaa gaaaacttga atttgatttt gccgaaatta taaatggcgg atatgatgta
     7201 gttttaacag caggtggaat tcaatccaat cacgcaagaa tgacagcggc agcagcacgt
     7261 aaacttgggt ttgatatgaa acttgttctt ggtggaccag attttgaaat tgttaaaggg
     7321 aatctgttac tagatatttt atttaattct gaaataagat atttagttga tgatgataca
     7381 aattcaagtc tagagtcaga aatgcaattg tgggcagatg agcttttaag tgaaggaaga
     7441 aaaccattta taattccaat tggtggaagt acagggcttg gtgctcttgg ttatgttaag
     7501 gcgatggaag aactatctca tcagtttgag aatgatgatg tgcaaatagt tgttggtgtt
     7561 ggttcttgcg gaacttttgc cggaacaatt ttaggcgcaa atatttttct tcctaaggca
     7621 cgggtaattg gcattagtgt ttcaaggaat tccgaagcga tcagacaaag aacattagag
     7681 ctgattgaag aaagtgccaa gctgataaat tttaatatag agaagcaaat atcaattgaa
     7741 tgctacgatg attattttat agaatatgga atgattactg aaaaaggaag gcaagcaatt
     7801 gacttatgtg caaatcttga agggatatta cttgatccga tttataccgg gaaagtaatg
     7861 gctggattaa ttgatcttaa agagaaaggt gtaattgata gaaagattcc tgtagtattt
     7921 attcataccg gaggattgcc aattattttt tcctttgaaa aggaatttgg aaacaaactt
     7981 aactgtacaa aaatatttcc tcagtagtaa ttaaatggtc gcaaaagttt taataattat
     8041 aatttgcttt atttactttt cacctcattc attcacacag gatgaaaaat cttttgaagc
     8101 aaaagaagta aaatcattaa tagaaaagtt ttttaatgag gatccatggg taaacggaga
     8161 agcaattggt aaactcgtta aaattggtga acctgctgtt gatgatttaa taatttcact
     8221 acaggataaa tatgaaaatg ttcgttggtg ctctgcaatc actttagaaa aaatttctcc
     8281 acttggaaga agatctattc catttctcat aaacgcatta acagatgaaa gcaaaaacgt
     8341 tcgctggtgt tcagcgttag cacttggcaa atttaaatct gaagcattaa aatctattcc
     8401 ggctttacaa aaacttttat acgatgatga ttacgatgta agatgggcag cttatgttgc
     8461 tattttaaaa attgataaaa aatctttaaa tgtaattcca gatcttttag aaaagattaa
     8521 tattgttgaa aaattaactc ctgaattaat gagtcaatta aaagtcccgg gtgtatcaat
     8581 tagcataatt cacaacaaca atattgctta ttcaaaatct tttggtgtcg cagatgcaga
     8641 tactaaatta cctgttgata tcaacactat gtttgaagct tgttcaatga gcaaaccagt
     8701 ttttacgctc ctggttcttc aattagtcga tcagggcaaa ttagatttag actctccttt
     8761 atctgattat ttacccgaaa catttgtaag tgatgatgat tattcaaaac taattactgc
     8821 aaggatgatc ttatcacaca cgggtggaat gcccaactgg cgaaagggag acgaagaaag
     8881 aaatgctcca attccaatat actttaaacc cggcacaaga tttaattatt cgggtgaagg
     8941 aatttattac ttacaaagag ttgttgaaaa aattactaat caatcgttag aaaattatgc
     9001 tagagaaaat ttgttcgata aacttggatt aacttcaaca agttttatct ggaaagaaaa
     9061 tttcgataag caaattgcaa cagggcacaa tgctgatggt aagtgcaatg aaagaaaaaa
     9121 atatgttcat agcaatgctg catatactct ttacacaact gcggatgaat atgcaaagat
     9181 tattgttgaa atgacgaatc ccaagataga aaatggatat tctctttcaa aaaacatggc
     9241 tgatgaaatg ttaactcatc agactcgtgt tgatactcgt gaagtgattg atagatctgg
     9301 aagaaacttc gggttatttg catttcgtgg attaggttgg gcgattgatt caacaattac
     9361 aggtgacgta gtatatcata gcggtgcaaa tcaaacagga tttaggtgtt attcccaatt
     9421 tagtcccaaa gacaaaagtg gaattgtgat tatgactaat ggtgaaaatg ggaatgaact
     9481 atggagaaga ttgattaaag aaattggtga tctgtagaga ttaaaaaatc aaatgtcatc
     9541 ctgagcttgt cgaaggatgg cgtaatttaa ttttatggtg tcacacttcg acaggctcag
     9601 tgtgacggaa atatttaaca ttgagtgtta ttatgaaaat agaaaagatt gaactccgcc
     9661 acataaaaat ggaattggtt tctccattca caacatcaat gggaacagag tacgatgaag
     9721 aacatattat tgttcgtgtt gatggtgaag gttatactgg ctggggcgaa agcgttgcag
     9781 aaggaactcc attttactct tacgaaactg ttacaacagc atggcatatt ttacaggatt
     9841 ttttaatccc tgcaattctt ggcaaagata tttctggtat cgatagcgcg atcgcttctt
     9901 atgaaaaagt acgcggacat atgatggcca aagcaggact tgaagctgca ctttgggatc
     9961 tgttcgcaaa atcaaaaaat atttctcttt caaaaatgat gggtggaaca agaaataaaa
    10021 ttgatgttgg tgttagcatt gggattcaat caactcctga agtcctggta aaaaaaatcg
    10081 aaggttatct tgctgaaggt tataagagaa taaaaattaa aattgctccc ggtaatgata
    10141 ttcaatttgt taaagcagta agaaaagaat ttccggatat cctatttcag gtggatgcta
    10201 actctgctta tgaattaaag catatagatt tattgaagca gatggataat cttaatttat
    10261 tattgattga acaacctctt gggtatgaag atatttatga tcactcaaag cttcagaaag
    10321 atcttaaaac tccgatttgt ctggatgaaa gcattcactc gttggatgat actcgtgctg
    10381 caattgaatt agatagctgc agagttataa atattaaacc cggccgtgtt ggcggattta
    10441 cagaatcaat acttatacac gattattgtg catcaaaaaa tattcctgtt tggtgcggcg
    10501 gaatgctgga aagcggaatc ggtagagcag gaaatgttgc gcttgcatca ttaccaaact
    10561 ttactttacc tggagatatt tctgcaagta aacgttatta caaagaagat attgttgaac
    10621 cggaatttgt tgttaacaaa gatggaacaa tggatgttcc aacaaaacca ggaattggtg
    10681 ttgaagtgaa tatgaagatg ttggagaagg ttacggttaa gaaaagagaa ttctaacttt
    10741 ttggtaaact attttccaat tcttcggtaa tatttgttac aaacaattca acttgctgtt
    10801 cgttgccaag taaatcctgt aaagtgattt tacttaataa accatcaagc attgtttgta
    10861 cagttttcca caaagagcgg agtgaacaat caatcgaatg agtgcagata ttttctactc
    10921 cagcgtgaag atcacaaaaa gtagattcat aaagttttcc gccaagagca gttaaaacat
    10981 caccaacaag aatttcgttt ggtgtacttg caagtttata cccgcctgtt tgtccgcgtg
    11041 aactttcaat gaatccggca aggcgtaatg cacgcaagat cttcccaaca tttgcttcag
    11101 aaagtttttc ctgctcactc agttcgggaa tagtcattcc tttatcggaa tcacttttcc
    11161 cgattctaag taatagtctt aaaccgtatt cttcttgtgt gctaaatttc atttttcttc
    11221 tgttctattg ccccgacctt taggtcgtgg ttaaaagtag taatagtagc tcggcttcag
    11281 ccacattttt aaaatattag aatgggacta aagtccattt ggtatctttt acaaggtcca
    11341 cgacctaaag gtcgtggcaa taattaccta tcctttaaac aatggtattt ttgatccaca
    11401 ttgttttgca tactgcactt ctgcaaattg ttctggcgta attgaaggac ccttcgcaga
    11461 acaaatttca ttaaaagtat tttttaaatt agttgcaatc tgctcagcgg aataaccttc
    11521 aatgttcaat ctgggtacaa agtggactaa ctcaccattt ttaaacaaag caatgcttgg
    11581 agatgagggt ggaaaacctt ttat
//
LOCUS       DASWBY010000226         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_383612, whole genome shotgun sequence.
ACCESSION   DASWBY010000226 DASWBY010000000
VERSION     DASWBY010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383612"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VF870_04100"
     CDS             complement(<1..305)
                     /locus_tag="VF870_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9251397.1"
                     /translation="MSENSSPESTPEQNTIKSITKEITQPFIDLFHSSRALWGINLSY
                     LLEGITYFGVVGYLVIYFTDFVKLDDISAGQMVGFQTAGITLAMLFLGATVDWIGVR"
     gene            complement(432..1340)
                     /locus_tag="VF870_04105"
     CDS             complement(432..1340)
                     /locus_tag="VF870_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9251398.1"
                     /translation="MIIENPKTPLTEIFNPLFEQKQIRFFIKREDLTDEFISGNKFYK
                     LKYNLIESEKLGFKTLLTFGGAYSNHIHATAAAGKKYGFNTIGIIRGEEHLPLNPTLS
                     SAQENGMTIEYLNRKSYRNKYDENLIKSFKEKFGEFYLIPEGGSNHLAIKGCAEIIPS
                     ITTDFNFVCSACGTGGTLAGLILGLDSKSFALGFSVLKGGSFLNQNIRTLLSYYQKEN
                     LNNFQINLDYHFGGYAKTNKELNNFCKEFYEQHNIRIEPIYTGKMFYGIYDLIRNNFF
                     PSGSTIVAIHTGGLQGLKGLKERKMI"
     gene            complement(1462..1731)
                     /locus_tag="VF870_04110"
     CDS             complement(1462..1731)
                     /locus_tag="VF870_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251399.1"
                     /translation="MENIIYPKTISNPLADLISDEIWELLNSRGLINDRSVRDYIIRR
                     RFKTLRSQKVRTGDAIDTLRAEYPYLQFDTIRKIVHNPPKQISRS"
BASE COUNT          597 a          299 c          287 g          635 t
ORIGIN      
        1 tttctaaccc caatccaatc aacggtggct cccaggaaca acatcgccag agttataccc
       61 gctgtttgaa atccaaccat ttgtcctgca cttatatcat caagcttaac aaaatcagta
      121 aagtaaataa ctaagtagcc aacaactcca aagtacgtaa taccttcaag caggtaggat
      181 agatttattc cccatagagc gcgagatgaa tggaaaagat ctataaaggg ctgagttatt
      241 tctttagtga tacttttaat cgtgttttgt tccggtgtgg attctggtga tgaattttcg
      301 gacataattg ttcctgtaaa aaaaatgatt aataattatt tagatcttaa aaaatagtta
      361 caaacaaaaa agaataaata aaataacaaa gcaaagttat aaaggatacg ctattaaagc
      421 ataagatata atcaaatcat ttttctttct tttaatcctt tcaaaccttg taacccgccg
      481 gtatggattg caacaatggt gctgcctgat ggaaaaaagt tattccttat caaatcataa
      541 attccataga acattttgcc cgtataaatt ggttctatcc ggatgttgtg ttgttcataa
      601 aattctttac aaaaattatt taattcctta tttgttttag catatccgcc gaagtgataa
      661 tccagattta tctgaaagtt attaagattt tctttttgat aataagacag aagggtgcgg
      721 atattttgat tgagaaatga accacctttt aggacagaaa atcctagtgc aaaagatttg
      781 gaatctaatc ctaaaatcag tcctgcaagc gtaccgcctg taccgcatgc agaacaaaca
      841 aaattgaaat cagttgtaat actgggaata atttctgcac agcctttaat tgcaagatga
      901 tttgatccgc cttcgggtat taaataaaat tctccaaact tttctttaaa tgatttaatt
      961 aaattttcat catatttatt tctataagat tttctattga ggtactcgat tgtcattcca
     1021 ttttcttgtg cagaacttaa tgtgggatta agcggtaaat gttcttcacc gcgaatgatt
     1081 ccgattgtat taaatccata ttttttacca gccgctgcag ttgcgtgtat atgatttgaa
     1141 taagctccgc caaacgtaag aagagtctta aagccaagtt tttcagattc aataagattg
     1201 tattttagtt tataaaattt attgccggaa ataaattcat ctgtgagatc ttcccgcttg
     1261 ataaaaaatc taatctgttt ctgttcgaat aaaggattga agatttctgt aagaggagtt
     1321 ttaggatttt ctattatcat aattactttc taaatcaaaa ttaatgaaat agaagttaaa
     1381 aggactaact aatatgttta aaaaagaaaa cccgttccga ttttttgatc gaaacgggca
     1441 agggcaaact ccttaaaagt tctatgatct acttatctgt tttggagggt tgtgcactat
     1501 ctttcttatt gtatcaaatt gaagataagg atattctgcc cttaaagtat caattgcatc
     1561 accggttcta actttttgtg agcgtaatgt tttaaatctt ctgcgaataa tataatctct
     1621 tactgagcgg tcattaatta aaccgcgact gttaagtaat tcccaaatct catcgcttat
     1681 cagatcagct aatggattag aaattgtttt tggatatatg atgttttcca taacatcctc
     1741 ctctatattg gtttgtaaag tttttatcct gtcgttttta ttattcatct tttattcttc
     1801 attaaaacat tctatcaa
//
LOCUS       DASWBY010000227         4987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_309245, whole genome shotgun sequence.
ACCESSION   DASWBY010000227 DASWBY010000000
VERSION     DASWBY010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309245"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VF870_04115"
     CDS             complement(<1..717)
                     /locus_tag="VF870_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HEX9251400.1"
                     /translation="MKSFKDIYKNSENKIGGIAAAPGIVIGKVYLFTKEKIEISKAPI
                     TNIEEAIGNFEEALRQSKKELNKIFGIAREKMDEVRAAIFEAQLMILDDPILLGNIEQ
                     RIKDEMIQPEFIVADEISKYQELMIISHESYMKERAHDIEDIKNRIIRNLQKKRWQSK
                     IEKNLIVVSEALTPADTLLFSRRKSLAFVTDHGGLTSHAAIISRSLNIPAVVGTHNAS
                     TLIKDGDLIIVDGFHGYIITN"
     gene            complement(714..980)
                     /locus_tag="VF870_04120"
     CDS             complement(714..980)
                     /locus_tag="VF870_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX9251401.1"
                     /translation="MIEKIVKIVNKAGLHTRPAATIVKLAAKYKSDFYIYRDGMSING
                     KSIIGVMTLAAEIGSELKLTFDGADEQQACDEIVAYFERGFDEL"
     gene            complement(980..1975)
                     /locus_tag="VF870_04125"
     CDS             complement(980..1975)
                     /locus_tag="VF870_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9251402.1"
                     /translation="MKPADVKKYISFYERERKIIDKKLALALKRRDPLSLYQPASYIL
                     ESSGKRLRPLLVLLSAKAIGGNIKNFYNAALSVEMLHNFTLVHDDIMDNADLRRGNLT
                     LHKKYNVNTAILAGDILAAVAYEFLLKDCKVNAKQALDSFTKGLVEVCEGQSLDTEFE
                     TRKNVSLPEYIKMIEKKTAALARMCCELGAYLANGSKEDIKALSNYGKYLGIAFQIQD
                     DLLDIIADKFEFGKRIGGDLLEGKKTFLFIEALEKAKGKDKDDLLKVIANKGIKENQI
                     EYYKKLYEKLGVIDDAKIAIRSYTNKALRSINKLSNKSEVEIFHWLADSLIKRNK"
     gene            complement(1972..3012)
                     /gene="fni"
                     /locus_tag="VF870_04130"
     CDS             complement(1972..3012)
                     /gene="fni"
                     /locus_tag="VF870_04130"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX9251403.1"
                     /translation="MSKSITETSKRKREHIDLCLTDEVSFKAKTNGFDRYDFIHNALT
                     EVDISKISFQTKLFSKKINYPFLISCMTGGTTEAENINQKLAVIAEELKIPIGVGSQR
                     QALENKDYHNSYSIIRKTAKSVPVFGNIGAAQLVQSNNLKDILGLINMLEADAFVVHL
                     NPLQELLQPEGEPNFVGLLKKLEKLVLKSQVRIVIKEVGSGISKEVAIRLLNIGVNGI
                     DVAGAGGTSWAGVEILRSKSKNDFWDWGLPTSYCLKEIYPLKKKYKFTLIGSGGINSA
                     DDLSMAFALGADVVASARVILQTLMKNGDEAVIILILNWFEYLKKVMFLTGSKNLSEL
                     QNKKIIKKENLF"
     gene            complement(3178..>4987)
                     /gene="pabB"
                     /locus_tag="VF870_04135"
     CDS             complement(3178..>4987)
                     /gene="pabB"
                     /locus_tag="VF870_04135"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEX9251404.1"
                     /translation="IINTVLYTPNAAFFYTPPIYGKSESYLFLKPKEIVTIKSLRNLD
                     DNLKRIDNLIADGLVGYSLMNYEAGYLFEKTLNKYLPKNKSLIQFFFYDKKDVQTIKS
                     NKILLDSSEKFQVKNFKLNTTKNEYELSIRKIRSYIEEGDTYQVNYTVKGKFDFNGSY
                     SSLFASLVFNQSARYTALINNGKNIIISLSPELFFEMNGKKITSKPMKGTSRRGIEIS
                     SDGFKKYELKRSEKNRAENVMIVDMIRNDLGRISEYGSVKVKNMFEVEKYESVFQMVS
                     TIHSRLKKQIKLSDVLKNIFPCGSITGAPKIRTMEIINELEKEHRGIYTGSIGLIRKN
                     KITFNVPIRTLCIDKKSGKGEIGLGSGIVWDSIAEEEYEETVLKGKFLSKDEKQFEIF
                     ETMLLKDGKIFLLDEHLDRMEQTANFFLFCFDRNKIKLQLNKIISRNSKDGCRIRISL
                     DKYGKLSYKFTEIPKLMSEMKVIISKNKIHSNNRFQYFKTTNRYLYNREYKLFAAKGF
                     LDVIYFNERSELAEGAITNIFIYKDDVISTPPLNTGILPGVYRKHLLKNNSGIRERKL
                     YLDDLLEADKIVLTNSVRGEVVVDKLFLDENEFIEF"
BASE COUNT         1546 a          905 c          641 g         1895 t
ORIGIN      
        1 attggtgata atgtaaccat ggaatccatc aacaataatt aaatcaccat ctttaataag
       61 tgttgaagca ttatgtgttc caacaacagc aggaatgttt aatgatctgg aaataattgc
      121 tgcatgagaa gtcaatccgc catgatcagt tacaaaagca agtgattttc tgcgagaaaa
      181 aagtaacgta tctgcaggag ttaaggcttc gcttacaaca attaagtttt tttcaatttt
      241 agattgccat cttttctttt ggagatttct aattattcta tttttaatat cttcaatatc
      301 atgcgcacgt tctttcatgt aagattcgtg agaaattatc atcaattcct gatacttaga
      361 aatttcatcc gcaacaataa actccggctg aatcatttca tctttaattc tttgttcaat
      421 gttgccaagt aatatcggat catcaagaat catcaactga gcctcaaaaa tagctgcacg
      481 aacctcatcc attttttcgc gtgcaattcc aaatatttta ttcaattcct tttttgattg
      541 tctaagagct tcttcaaaat tgccgattgc ttcttcgata ttagtaattg gagctttact
      601 gatttcaatt ttttcttttg taaataaata tactttacca attacgattc ccggtgcagc
      661 agcaattcct ccaattttat tttcagaatt tttataaatg tctttaaatg atttcataat
      721 tcgtcaaatc ccctttcaaa ataagctact atttcatcac aggcttgctg ttcgtctgcg
      781 ccatcaaaag ttaacttcaa ttcagaaccg atttcagcgg ctaaagtcat tactccaatt
      841 atactttttc catttatact cataccatct ctataaatgt aaaaatcact tttatatttt
      901 gcagcaagct taacaatagt tgcagcggga cgtgtatgca gtccggcttt gtttactatt
      961 ttaacaattt tttcaatcat tatttatttc ttttgattaa tgaatcagcc aaccagtgaa
     1021 atatttccac ttcactttta tttgatagtt tattaattga tcttaatgct ttattagtat
     1081 aactgcgtat tgcaattttt gcatcatcaa taacaccaag tttttcataa agttttttat
     1141 aatactcaat ttgattttct tttatacctt tatttgcgat aacttttaat aaatcatctt
     1201 tatcttttcc ctttgccttc tctagggcct ctatgaataa aaatgttttt ttgccctcca
     1261 ataaatcgcc gccgattctt ttgccaaatt caaatttatc cgctataata tccaaaagat
     1321 catcctgaat ttgaaatgct attccaagat attttccgta attagataaa gctttaatat
     1381 cctctttgct tccatttgca agataagcac caagctcaca gcacattctt gcaagtgcag
     1441 cagttttctt ttcaatcatc ttaatgtatt caggcagtga aacatttttt cttgtttcaa
     1501 attctgtatc gagactctgc ccttcgcaaa cttcaaccaa tccttttgta aaggaatcca
     1561 gtgcttgttt tgcatttact ttgcaatcct ttaataaaaa ttcataagct acagctgcaa
     1621 gaatatcacc cgcaagaatt gctgtattaa cattatattt tttatggagt gtaagattac
     1681 ctcttcttaa atctgcatta tccattatgt catcatgcac aagagtaaaa ttgtgcagca
     1741 tctcaacaga aagtgcagcg ttgtaaaagt tttttatatt accgccgatt gctttagcag
     1801 atagtaaaac cagcaaaggt cttaatcgtt taccgctgct ttcaagaata tatgatgcgg
     1861 gctgataaag tgatagtggg tctcttcgtt ttaatgccaa agcaagtttt ttatcaatta
     1921 tttttctttc tctttcataa aacgaaatat atttttttac atcagcgggt ttcaaaatag
     1981 attttccttt ttaataatct ttttattctg tagttcactt agattttttg aaccagttaa
     2041 aaacataact tttttaagat actcaaacca atttaatatt aatattatta cggcttcatc
     2101 gccatttttc atcaatgttt gcaaaataac tcttgcagaa gcaacaacat cagcacctag
     2161 tgcaaaagcc atagataaat catctgctga attaattcca cccgaaccaa ttaaggtaaa
     2221 tttatatttt ttctttagtg gataaatttc ttttagacaa tatgatgtcg gtaagcccca
     2281 atcccaaaaa tcattttttg atttacttct taaaatttcc acacccgccc aactcgttcc
     2341 gcctgctcct gcaacatcaa ttccgttcac acctatattc aataatctta ttgcaacttc
     2401 tttactgata cctgacccaa cttctttgat aacaatacgt acctgagatt ttaaaactag
     2461 tttttctaat tttttcaata acccgacaaa gtttggttca ccttctggct gcaataattc
     2521 ctgtaaagga tttaagtgaa caacaaaagc atcagcttca agcatattaa tcaatcctaa
     2581 aatatcttta agattatttg attgaaccaa ctgtgcagca cctatatttc caaacacagg
     2641 aacactctta gcagttttac gaatgatact gtaagaatta tggtaatctt tattttctaa
     2701 tgcttgcctt tgacttccaa cacctatagg aattttaagt tcttcagcaa taacagctaa
     2761 tttttgatta atattttctg cttcagtcgt tccgcctgtc atgcaggaaa taagaaatgg
     2821 atagtttatt ttttttgaaa ataatttcgt ttgaaaagaa attttggaaa tatcaacttc
     2881 tgtcagtgca ttatgtatga aatcatatct gtcgaatcca ttggttttag ctttaaaaga
     2941 aacttcatcc gtgaggcaca aatcgatgtg ttctcttttt cttttcgatg tttctgtaat
     3001 ggatttagac atttctgtgt tgataattta tttgtttgca aaatatggaa aataaaataa
     3061 atatgctgtt gaatgtttac ttggaattaa tcctattagc actctttgtg ccagccatta
     3121 tgtgccttgt ggttagtctt ttttacctga aactgcacaa agcaaacaaa gtgcttatta
     3181 aaattcaata aactcatttt catcaagaaa taatttatca acaacaacct caccacgcac
     3241 tgagtttgtt agaacgattt tatccgcttc cagcaaatcg tctaaataaa gttttctttc
     3301 tcttatacca gaattatttt ttaacagatg ttttctatag actccaggta atattccagt
     3361 attcagtggt ggcgttgata ttacatcatc cttataaata aaaatattag taattgcacc
     3421 ctctgctagc tcagatcttt cattaaaata gataacatct aaaaatcctt ttgctgcgaa
     3481 taatttatat tctcgattat acaagtatcg attagttgtt ttaaagtatt gaaatctatt
     3541 gtttgaatga attttgtttt ttgaaataat tactttcatt tcactcatta attttggaat
     3601 ctcagtgaac ttatatgaaa gttttccgta cttgtctaga gaaatcctta tacggcagcc
     3661 gtcttttgaa ttacgggaaa tgattttgtt taactgtagt ttgattttat ttctatcaaa
     3721 acagaataaa aagaaattgg cagtttgctc cattctatcc aaatgttcat caagcagaaa
     3781 tatttttcca tcttttaaaa gcatcgtttc aaatatttca aattgttttt catcctttga
     3841 taaaaattta ccttttaata ctgtttcttc gtactcttct tcagcaatgc tgtcccaaac
     3901 aattccgctt cctaaaccga tctcaccttt acctgatttt ttatctatgc acaatgttct
     3961 aatcgggaca ttaaaagtaa ttttattttt tctgattaaa ccaatcgatc ctgtgtaaat
     4021 tccacggtgt tctttttcaa gctcattaat aatttccata gttcgaattt ttggtgcacc
     4081 cgtaatagac ccacaaggaa agatattttt taatacatca gaaagtttta tttgcttttt
     4141 aagcctggaa tgaattgtgc taaccatctg aaaaactgat tcatactttt caacttcaaa
     4201 catattttta acttttactg aaccatattc acttattctt ccaagatcat ttctgatcat
     4261 atcaacaatc attacatttt cagcacgatt tttttcactt cttttcagct catatttttt
     4321 aaatccatca ctcgatattt caattcctct tctcgaagta cctttcatcg gtttggaagt
     4381 aatttttttc ccattcattt caaaaaatag ttccggtgaa agtgaaataa ttatattctt
     4441 cccattattt ataagagctg tatatcgtgc agattgatta aatactaagc tagcaaacaa
     4501 acttgagtat gaaccattaa aatcaaactt gcctttaact gtgtagttaa cctggtaagt
     4561 atcaccttcc tctatataag atctgatttt tctgatagat agttcatatt cattcttagt
     4621 tgtattcagt ttgaagttct taacttgaaa cttttcagac gaatctaaca atattttatt
     4681 tgatttaatt gtttgaacat cttttttatc ataaaaaaag aattgaatca agcttttatt
     4741 ttttggtaaa tacttattta aagttttctc aaataaatag cctgcttcat aattcatcaa
     4801 agaatatcca accaaaccat cagcaataag attatctatt cgttttaaat tatcatccag
     4861 atttcttaaa gattttattg taacaatttc ttttggtttt aggaaaagat atgattcact
     4921 ctttccataa attggcggag tgtagaagaa agcagcgttg ggagtatata aaactgtatt
     4981 tatgatc
//
LOCUS       DASWBY010000228         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_123751, whole genome shotgun sequence.
ACCESSION   DASWBY010000228 DASWBY010000000
VERSION     DASWBY010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123751"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VF870_04140"
     CDS             <1..312
                     /locus_tag="VF870_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251405.1"
                     /translation="KKYFLKYLRKDDYNRNDIAQLLSFKELVKLFSKDFEIKLFTKFA
                     VLELSKRNKGIVWSNFHLKLIGFCKIVKLDWLIDKIANDKIGIINKYFTPTFYMIIKR
                     K"
     gene            474..869
                     /gene="cdd"
                     /locus_tag="VF870_04145"
     CDS             474..869
                     /gene="cdd"
                     /locus_tag="VF870_04145"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX9251406.1"
                     /translation="MDYKTLAHKAVEAKVNALPTYSNFHVGAALLTMEDKIFQGCNVE
                     NSTYGLTICAERTAIFKAISEGERKFKAIAIASDSHDYITPCGACRQIIFDHCGEIDI
                     ICTNGKEEYKVFKTSELLPFAFGDKDLNK"
     gene            872..1453
                     /locus_tag="VF870_04150"
     CDS             872..1453
                     /locus_tag="VF870_04150"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX9251407.1"
                     /translation="MEPTPHAVPRETGWIEVITGCMFSGKTEELIRRLRRAKIAKQKV
                     VIFKPNIDTRYSTNSIVSHSEQSLPSILIKDVKEILDLIEDAQVIGIDEAQFFSNDLI
                     DVCNKLADDGKRVIVAGLDMDYRGVPFEPMPQILSVAEYITKSLAICVECGNPADRTQ
                     RKTTSSERVVVGAADVYEARCRKCHYIPTEENH"
     gene            1453..>1637
                     /locus_tag="VF870_04155"
     CDS             1453..>1637
                     /locus_tag="VF870_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HEX9251408.1"
                     /translation="MNLESFIRGIFGLALLIGIAFLISNNKKKINWRLVISGFGLQII
                     FAILILRGDSLRNYFSPL"
BASE COUNT          572 a          249 c          291 g          525 t
ORIGIN      
        1 aaaaaatatt ttcttaagta tctccgcaaa gatgattata accgtaatga tattgcacaa
       61 cttttatcgt ttaaagaact cgtaaaatta ttcagcaaag attttgaaat aaaactattt
      121 acaaaatttg ctgttttaga attgtctaaa agaaataaag gaattgtctg gagtaatttt
      181 catcttaagc ttattggttt ttgtaaaatt gtaaaacttg actggcttat agataaaatc
      241 gcaaatgata aaattggaat tattaataaa tattttacac caacatttta tatgattatt
      301 aaacgcaaat gataattatt tctgccaatg gcattccttc gaaagaatga agtatcttgc
      361 atgactgagg aatctgcaga tcttttttgt ttaagacttc taactcgggt tgaaatgaca
      421 aataaattaa atttgaacta aaacatttta agaatatttt tccaagtaaa attatggatt
      481 ataaaacatt agcacataaa gcggttgaag caaaagtaaa tgcgcttcct acctattcga
      541 attttcatgt tggggccgca ctgcttacaa tggaggataa aatttttcaa ggctgcaacg
      601 ttgaaaacag tacgtatggc ttaacaattt gtgctgaacg cacagcaatc ttcaaagcaa
      661 tttctgaagg tgaaagaaaa tttaaagcta ttgccattgc tagtgattca catgattaca
      721 tcactccttg tggtgcatgc agacaaatta tttttgatca ttgcggtgaa attgatatta
      781 tctgcacaaa cggaaaagaa gagtataaag tttttaaaac atcagaatta ctgccatttg
      841 cttttgggga caaggattta aataaataat tatggaaccg acaccacacg cagtaccaag
      901 ggaaaccggc tggatagaag taataaccgg ttgtatgttc agcggaaaaa ctgaagagct
      961 aatacgaaga ttacgcagag caaaaatcgc aaaacaaaaa gttgtgattt ttaaaccgaa
     1021 catcgacaca agatattcta caaattcaat tgtatctcac agcgagcaat ctttgccatc
     1081 gattttaatt aaagatgtaa aagagatttt agatcttatt gaagatgcac aggtaattgg
     1141 tattgatgaa gctcaatttt tttctaacga tctaattgat gtttgtaata aacttgctga
     1201 tgatggtaaa cgcgttatag tcgcaggctt ggatatggat taccgaggtg ttccatttga
     1261 accgatgccg cagatacttt ctgttgcaga gtatattaca aaatcgcttg ctatttgtgt
     1321 tgaatgcgga aatcctgccg acagaaccca aagaaaaact acatcatcag aacgtgttgt
     1381 tgttggagcc gctgacgttt atgaagcaag atgccgtaaa tgtcattaca ttccaactga
     1441 agagaatcat taatgaattt agaatcattc attcgtggta tttttggttt agcattatta
     1501 ataggaatcg catttctaat ttccaacaat aagaaaaaaa taaattggag attagttatc
     1561 tcgggattcg ggcttcaaat aatttttgca attcttatcc ttcgtggtga ttcgctacga
     1621 aattattttt cccctct
//
LOCUS       DASWBY010000229         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_722095, whole genome shotgun sequence.
ACCESSION   DASWBY010000229 DASWBY010000000
VERSION     DASWBY010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722095"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF870_04160"
     CDS             <1..258
                     /locus_tag="VF870_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX9251409.1"
                     /translation="NTYELGMAAIDLGAGRKTKDDKIDPKAGIVFNYKIGDIINKGVV
                     LAKLYSDSKSGIDNANKRIESSIKISKSKPQKLKLIKTIIN"
     gene            370..1242
                     /locus_tag="VF870_04165"
     CDS             370..1242
                     /locus_tag="VF870_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEX9251410.1"
                     /translation="MIFILVIIIAVAAFFVYRNAKKYNIKQQANFALIGMVVAIFLAF
                     SQMFTVIPAGHVGVIDFFGNVSDQTLLPGVNFVNPLANVIKFDARTQELKEEMSVPSK
                     EGLSVQLEMSLLYSLSFQNANKIYKSVGEDYVEKILVPQFRSVVRGVTSRYEARSLYT
                     AEREKLAKQMEEELTALVGPRGITIESAPLRKITLPPGLTASIEEKLKAEQESQRMEF
                     ILTREKQEAERKRIEAKGIADFQEIVSKGISEQLLKWKGIEATEKLAGSNNTKIVVIG
                     SGKDGLPLILGGDK"
     gene            1316..2140
                     /gene="ccsA"
                     /locus_tag="VF870_04170"
     CDS             1316..2140
                     /gene="ccsA"
                     /locus_tag="VF870_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX9251411.1"
                     /translation="MIPAIHTINLLLPFFYIATFAIYFYDFQQGGRKFENSKRLFLFL
                     TLIFHFFYLLLRTIEFNHPPITNVFEIFTVLAFAISFSYFILELVTDIRGTGPFIIII
                     SIIFQLISTLFILDLTEVKEVLRSNLLGIHVISALIGYSGITISAVYGVLYLILYNEI
                     KLNKFGLIFNRLPSLEILERLSFIAAIIGFVFLTITIIIGIIWLPQAFPKITFLDPKL
                     IGTFAVWILYGIGLINKFLGKWRGKKLIILSIIGFTLAILSTMLTNFLAKSFHSFY"
     gene            2144..>2551
                     /locus_tag="VF870_04175"
     CDS             2144..>2551
                     /locus_tag="VF870_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX9251412.1"
                     /translation="MNLLGISINHRTAPVELREALHLSEDEIRAFIQQTKEKAFAEGI
                     IISTCNRTEIYAIPKNDKTSLIDLQNLIKNYKSSYEVKDENFQHFISRESIEHLFRVA
                     CGIDSLLVGDNQIFKQVKDSFLISEEMNFAGVLI"
BASE COUNT          881 a          375 c          456 g          839 t
ORIGIN      
        1 aacacttatg aacttggaat ggctgcaata gatttgggtg ctggtagaaa aacgaaagat
       61 gataaaattg atcctaaagc tggaatagtt tttaattata aaattggtga cattattaat
      121 aaaggtgttg tacttgctaa gttatattct gattctaaat caggaattga taatgcaaat
      181 aaaagaattg aatcatcaat taaaatttca aagtcaaaac cacaaaagct aaaactgatt
      241 aagactatta ttaattagtg ggcctttaag ttgatttcat cccgagatag actctatgga
      301 ttcactttca attcatatca ctcaatagta tattttacaa cacgaatatc aaatctttag
      361 gagtaagtaa tgatttttat cttagtaata ataatagcag tagctgcgtt ttttgtgtac
      421 aggaatgcaa agaaatacaa tatcaaacaa caagcaaatt ttgcattaat aggaatggtt
      481 gttgccatat ttttggcttt ttcacaaatg tttaccgtta tccctgccgg tcatgttggg
      541 gtaatagatt tctttggtaa tgtaagtgat caaactcttt tgccgggtgt aaactttgtt
      601 aatcctttag ctaacgtaat aaagtttgat gcaagaactc aggagttaaa agaagaaatg
      661 tcagtgccat cgaaagaggg gttaagtgtg caattggaaa tgagtttgct ttactcttta
      721 agtttccaga acgcaaataa aatttataaa tctgttggtg aagattatgt agaaaaaatt
      781 ctcgttcctc aatttcgttc cgttgtaaga ggtgtaacct ccagatacga agcgcgctca
      841 ctttacaccg cggaaagaga aaaattagca aaacaaatgg aagaagaact aactgcgtta
      901 gttggtccgc gtggtattac gattgaatcc gcaccgctga gaaaaattac tttgccgccc
      961 ggattgacag catccattga ggaaaagtta aaagccgagc aggaaagcca aagaatggaa
     1021 ttcattctaa ccagagaaaa acaggaagct gaaagaaaac gtattgaagc aaaaggaatt
     1081 gctgattttc aggaaattgt atccaaagga ataagcgaac agcttttaaa atggaaggga
     1141 attgaagcaa cagagaaatt agcaggctca aataatacaa aaattgtggt aattggttca
     1201 ggaaaagatg gattaccatt aattcttgga ggcgataaat agaccagtca gtcatttgtt
     1261 attttaggca attttagtaa atttgaactc acgagtcaat aaataattta aaaatatgat
     1321 tccggcaata catactataa atctgttact tccgtttttt tatattgcaa cttttgcgat
     1381 atatttttat gattttcagc aaggcggaag gaagtttgaa aattccaaaa gactttttct
     1441 tttcttaaca ctcatatttc atttttttta tctgctgtta agaacaattg aatttaatca
     1501 tccgccgatt acaaatgtgt ttgaaatctt tactgtgctt gcattcgcaa taagtttttc
     1561 atatttcatt ttggaattgg taacagatat aagaggaacc ggaccattta taattatcat
     1621 ctcaattatt tttcaactaa tatctacatt atttattctg gaccttacag aagtaaaaga
     1681 agttttaaga agtaacttac tgggcattca tgttatcagt gcattaattg gttattccgg
     1741 aataactatt tccgcagtgt atggagtttt atatttaatt ctttacaacg aaataaaact
     1801 taataaattt ggtttgatat ttaatcgatt accaagttta gaaattttag aaagattaag
     1861 ttttatagca gctattatcg gatttgtttt tttgacgatc acaataatca taggaattat
     1921 ttggctgccc caggcttttc ccaaaatcac atttcttgat cccaaactta ttggaacctt
     1981 tgcggtttgg atcctgtatg gaattggatt aattaataaa ttccttggca agtggcgcgg
     2041 taagaaatta ataattttat caataattgg atttacgctt gcgattttat caacaatgct
     2101 cacaaatttt ttagcaaaaa gttttcactc tttctattag aatatgaatc ttcttggaat
     2161 atcaataaat catcgaactg cccccgtaga actcagagag gcacttcact taagtgaaga
     2221 tgaaatccgt gcgttcatac agcagactaa agaaaaagct tttgcagaag ggattattat
     2281 ttccacttgc aacagaacag agatttatgc aattccaaag aatgataaaa caagcttaat
     2341 agatctgcag aatttgatta aaaattataa atcaagttat gaagtaaagg atgaaaactt
     2401 tcagcatttt atttcccgcg aatcgatcga acatcttttc cgtgttgctt gcggaataga
     2461 ttcgttgttg gtcggtgata accagatttt taaacaggta aaagattcat tccttatttc
     2521 agaagaaatg aattttgccg gagtattgat t
//
LOCUS       DASWBY010000230         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_687319, whole genome shotgun sequence.
ACCESSION   DASWBY010000230 DASWBY010000000
VERSION     DASWBY010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687319"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VF870_04180"
     CDS             <1..308
                     /locus_tag="VF870_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9251413.1"
                     /translation="IALIFNPSIIILDEPTAGLDPVASSNLKQRILEERTKGKTIILT
                     SHIMSEIEELADNILFLIDGRIVFDGEVKDLIESTGEHKLEKAIANMMTENENGTHN"
     gene            292..1080
                     /locus_tag="VF870_04185"
     CDS             292..1080
                     /locus_tag="VF870_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9251414.1"
                     /translation="MGLITKIIKFELSNTFRSKWIIVYSAFFLLLTFLLFNFEEDTSK
                     AALSLMNIVLIVIPLVSIIFGTMYVYNSLDYIQMVLCQPINRKSLFVGLYLGNSIPLA
                     LGFLFGTLLGTILSGYSFIDNKSIFILIVIGFGLTFIFTSIAFWIAIKFSDKARGLGI
                     SIIIWLMLSVVYDGLVLFVIYYFREYPIENIIIGVSLLNPIDLARIFFILDFNVSALM
                     GYTGALFNKFFGSSLGTILSIAMMFLWFVVPVLSGLKSFYKKDF"
     gene            1086..1511
                     /locus_tag="VF870_04190"
     CDS             1086..1511
                     /locus_tag="VF870_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9251415.1"
                     /translation="MNSFFSKKCEYGLQAVLYLTAQDAEFLCSAEEISSKLKIPKEFT
                     SKILQDLTEKGIIISRKGREGGFKLAIDPNQIKLIDIVEAIDGLDILKNCVMGFQNCN
                     KETKCPIHSEWMEIVNKAYRMLSEKTIDKLIVKSFNIII"
     gene            complement(1594..>2297)
                     /locus_tag="VF870_04195"
     CDS             complement(1594..>2297)
                     /locus_tag="VF870_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251416.1"
                     /translation="EFKKCIQELKLIIDELIATNDDVIVNVKDILKFGKLASNVFPNA
                     TLEEKRIILNSLGLNLLIKDRKLQIQFEKLLSLLKEISSSVKGKRVKKGRLEPAQVVI
                     KKGIYSDKDTINPLGLRKHDTDSKKNRLELPLFIAKSTNGRLKLTIDREELPNYLDLS
                     LKKCYQLTEGFYLPEIKIINNPRTKIDYKILAGEYKLLIDNGTVLNQAALARRLNVSR
                     AWVTKVCRIANNIED"
BASE COUNT          735 a          352 c          369 g          841 t
ORIGIN      
        1 acatcgcatt aatatttaat ccgtcaataa taattctgga tgaacccact gctggacttg
       61 atcctgtagc aagcagcaat cttaagcaaa gaattttgga agagcgcacc aaagggaaaa
      121 caataattct tacatctcac ataatgagtg agattgaaga acttgcagat aacattttgt
      181 tcctcatcga tggaaggatt gtattcgatg gtgaagtgaa agatttaatt gaatcaactg
      241 gtgaacataa attagaaaaa gcaattgcta atatgatgac agaaaacgaa aatgggactc
      301 ataactaaaa ttatcaaatt cgaactgagt aatactttca gaagtaagtg gataatcgtt
      361 tattctgctt tctttctgct gcttacattt cttctgttca attttgaaga agacaccagc
      421 aaagcagcac tcagtttgat gaacattgtt ttaatcgtaa tacctctagt aagcattata
      481 ttcggaacta tgtacgttta taactcctta gattacattc aaatggttct ttgtcagcct
      541 ataaacagga aatctttatt tgttggatta tatcttggta attctattcc tttagcatta
      601 ggatttttat tcggcacatt gcttggaaca atcctgagtg gatattcgtt tatcgacaat
      661 aaatcaattt ttattttaat tgtaataggt tttggactta cgtttatttt tacttcaata
      721 gccttttgga tagcaattaa gttttctgat aaagcaagag gacttggaat atcaataatc
      781 atctggctaa tgctttctgt tgtttatgat ggtttagttt tatttgtaat ctattatttc
      841 agagaatatc caattgaaaa tattataatt ggggttagtc ttttaaatcc aatcgatttg
      901 gcgagaatat ttttcatact cgatttcaat gtctcagcat tgatgggcta cactggtgca
      961 ttattcaata agttctttgg cagctcgctt gggactattc tttcaatagc tatgatgttt
     1021 ttgtggtttg tagttccagt attatccgga ctaaaaagtt tttacaaaaa agatttctga
     1081 tttttatgaa cagtttcttt tctaaaaaat gtgaatacgg cttacaggca gttttgtatt
     1141 taacggctca agatgctgag ttcttatgct ctgctgaaga aatatccagc aaactaaaaa
     1201 taccaaaaga atttacatct aaaatcttac aagatctgac agagaagggg ataatcattt
     1261 cacgcaaagg aagggaagga ggatttaaac ttgcaataga tcctaatcaa attaaactta
     1321 tcgatatagt tgaagcaata gatggattag atatcttaaa aaattgcgtg atgggttttc
     1381 aaaattgtaa taaagaaacg aaatgtccca ttcattcgga atggatggaa attgttaata
     1441 aagcatatag gatgttaagt gagaaaacga ttgataaatt aattgtaaaa tcatttaaca
     1501 taattattta attgttaata ggttaaacca aaataacatt accattttaa aaatattttc
     1561 tgctaagcaa ttctcaaaac aaagctttcc attttaatcc tcaatattat ttgctattct
     1621 acatactttt gtaacccaag ctctgcttac atttagtctt cttgcaagag cagcttggtt
     1681 taaaactgtt ccattatcaa ttaataattt gtactcacct gccaatattt tgtaatcaat
     1741 tttggttctt ggattattta ttattttgat ttcaggtagg taaaatccct ctgttaactg
     1801 ataacatttc ttaaggctga gatccaaata atttggtaat tcttctcgat caatggtcaa
     1861 tttgagtcta ccatttgttg attttgcgat aaaaagaggc aattcaagcc tatttttttt
     1921 tgagtcggta tcgtgtttgc ggagccctaa gggatttatg gtgtctttgt cactgtaaat
     1981 cccttttttt attactactt gcgcaggttc taaccgacct tttttcactc ttttaccctt
     2041 tactgaactt gatatttctt ttagtaaaga aagtagcttt tcaaattgaa tttgcaactt
     2101 tctgtccttt attaagaggt tcaaaccaag tgagttcaaa ataattcttt tctcttcaag
     2161 tgttgcattc ggaaatacat tagaagctaa tttcccaaac ttcaatatat cttttacatt
     2221 cacaatcaca tcatcattag tggcaattag ttcatcaata attaatttta attcttgtat
     2281 gcactttttg aactctg
//
LOCUS       DASWBY010000231         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_309668, whole genome shotgun sequence.
ACCESSION   DASWBY010000231 DASWBY010000000
VERSION     DASWBY010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309668"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..834
                     /locus_tag="VF870_04200"
     CDS             139..834
                     /locus_tag="VF870_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9251417.1"
                     /translation="MSLIELNQVSKIYDETIIPIHAVDNVDLKIEKGEFTAIVGPSGS
                     GKTTMLNLIGGLDQPTIGKIIVDGIDISTFKPDALINFRLHHIGFVFQAYNLIPVFTA
                     KENVEFIMLLQNIPKTEREKRAIEFLEAVGLKDRINSTPAELSGGQQQRVAVARALAS
                     TPSFVLADEPTANLDSASAESLLDLMEELNHKHNMTFVFSTHDHKVIKRAHRIITLQD
                     GKIKTDELNPLNS"
     gene            836..>1931
                     /locus_tag="VF870_04205"
     CDS             836..>1931
                     /locus_tag="VF870_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251418.1"
                     /translation="MKNKIIYIFILFSFYATAQSDSYSLGGYAKYLFSSVKYPQFNER
                     FYDHLIHTRLNTKWYPYSSLTATMELRFRTYFGGSVENYSNYEELIKSYQPLMDLDAV
                     LWSNESSIGYLEADRLYLDYYAGSLQTTLGRQRIAWGTSWVWNPTDIFNPQSVLDFDH
                     EELPGVDALRFQYYTGAVSKVEVALAPAREKELSSYGLLLSQNAFDYDFNFMGGLKRE
                     RWIIGTSWSGDILDAGFRGEITISQKPKTITQYDSIYSFFGEEAISANQNNVINFVLS
                     GDYTFPNSFYIHTELLFNSNGKTENVGLFADEAYQLGMLSAAKWSLYQEFSYDITPLM
                     RGSIFSIINPDDKSFVLVPSISYSIITNLDI"
BASE COUNT          632 a          337 c          347 g          615 t
ORIGIN      
        1 cttatttacc tttttcattc taccctcccc ttgcgttaat gattcttttc acatcagtaa
       61 ttgcagcttt gtatcctgcg cttaaagcaa tcagattaaa tcctgcaaca gcaataagaa
      121 catactagtg gagaaaatat gtctttaatt gaattgaatc aggtatcaaa aatttacgat
      181 gaaactatta ttcctataca tgccgtagat aatgttgatt taaaaattga gaaaggtgaa
      241 ttcactgcaa tagtcggtcc atccggttcc ggtaaaacta caatgcttaa tttaatcggt
      301 ggattagatc agccaacaat cggaaagatt attgtagatg ggatagatat ttcaaccttc
      361 aaacctgatg cgttaattaa cttcagatta catcatattg gatttgtatt tcaggcgtat
      421 aatcttattc ccgtctttac ggccaaagaa aatgttgagt ttataatgct gctgcagaat
      481 attccaaaaa cagaaagaga aaaaagagca atagagtttt tagaagcagt cggtttaaaa
      541 gatagaatta atagtactcc ggctgaactt tcaggcgggc agcagcaaag agtagcagtt
      601 gcacgtgcac ttgcttcaac cccctctttt gtccttgctg atgaaccgac agctaatctc
      661 gattctgcaa gtgctgaatc attactggat cttatggaag agttaaatca taagcataat
      721 atgacatttg ttttttctac tcatgatcac aaagtgatta agcgtgcaca tagaataatt
      781 acactccagg atggtaaaat aaaaaccgat gagttaaatc ctttaaacag ttaaaatgaa
      841 aaataaaatt atttatattt ttattttatt ttcattctac gcaactgcac aatctgattc
      901 atattcatta ggaggttatg caaaatatct tttcagttca gtaaaatatc ctcagtttaa
      961 cgaacgattt tatgatcact taatacatac aaggcttaat actaaatggt atccatattc
     1021 cagtctaaca gcaactatgg aattaagatt cagaacttat tttggaggaa gcgttgaaaa
     1081 ttattccaat tacgaggagc taataaaatc atatcagcct ttaatggatc ttgatgctgt
     1141 tttatggagt aacgaatcat caatcggcta tcttgaagcg gacagattat atcttgatta
     1201 ctatgcaggc agcttacaaa ctacgcttgg aagacagcga attgcatggg gaacaagctg
     1261 ggtctggaat cccactgata tttttaatcc ccaatctgtt ttagattttg atcatgaaga
     1321 attacccggt gttgatgcat taaggttcca gtattacacg ggagcagtaa gtaaagtaga
     1381 agtggctctg gcacccgcaa gagaaaaaga attatcctca tacggtttac tgctttctca
     1441 gaatgcattt gattatgatt ttaactttat gggcggattg aaacgtgaaa gatggataat
     1501 cggaacatca tggagcggag atattttgga tgcaggattc cgtggtgaga taaccatttc
     1561 tcagaaacct aaaacgatta cacaatacga ttccatctac tcattctttg gtgaagaagc
     1621 gatttctgca aatcagaata atgttatcaa ttttgtttta tccggtgact acactttccc
     1681 aaattcattc tatatacata ccgaactact atttaacagt aacggaaaaa cagaaaatgt
     1741 cgggttattt gctgatgaag cttatcagct tggaatgtta agtgcggcaa agtggtcatt
     1801 gtatcaggaa ttttcttatg acatcactcc gcttatgcgg ggatcaattt tttccattat
     1861 aaatccggat gataaatcat ttgtactcgt tccctcaatc agctattcca taataacaaa
     1921 tcttgatatt t
//
LOCUS       DASWBY010000232         1926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_159618, whole genome shotgun sequence.
ACCESSION   DASWBY010000232 DASWBY010000000
VERSION     DASWBY010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159618"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..1046
                     /gene="mltG"
                     /locus_tag="VF870_04210"
     CDS             12..1046
                     /gene="mltG"
                     /locus_tag="VF870_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX9251419.1"
                     /translation="MSIKLPNIFSKLQWALIAAVFIALVFIGYITFFTSNHFAKPAPY
                     TFEIKDGETFSSVTKRLFNEGIIPSKFNFKAAAYIYDAEKKIKAARFKIKNDLSYLDL
                     LDLLVNGPADYLRKIRINDGQTLKWLGAKVKRDVKIDSSAFVNLAKDKEFIKSLGLNV
                     STLEGYLFSKTYKIYENSSPEEAIKIFYDSFKEFFNDSLKQRAKQIGLTVHQVLTLAS
                     IIKGETDHVEEMPTIAGVYHNRLRIGMRLQADPTIQYLLPGGWRRLTYKDLQIESPYN
                     TYKYSGLPPGPINNPGKNAIMAVLYPEKNNYLYFVANGNGGHNFARSYSDHLKNVAKY
                     RRWLQTQNNQ"
     gene            1019..>1926
                     /locus_tag="VF870_04215"
     CDS             1019..>1926
                     /locus_tag="VF870_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9251420.1"
                     /translation="MVTNTKQSIKIFFIYFSIFFLAGCANQLPPGGGEIDTIPPVIIS
                     SYPENGTTNFKDDYIELEFSEYVDKRSFKDALFISPAIDGQPEISWSGKSVEINFPKG
                     LKDSLTYVVTIGTDVVDVNNKNRMANSFSFSFATGSLIDKRSIDGKVYGKNVEGTLIF
                     AYKYSDDTTKYLYKKPDYLSQIGKDGSYKLTGLAEAVYLVFAVKDEFRDLLYQADQDW
                     IGMPSKEISLTNTDSSFTGLNFFLMKVDTVKPRILGTVMTDKNHFVITLSEECDSSTY
                     LANNFQIIDSTSNQIIPVEYSYHNKS"
BASE COUNT          670 a          282 c          341 g          633 t
ORIGIN      
        1 ataactaaaa attgtcaata aaacttccta acatattcag taaacttcag tgggctttaa
       61 ttgcagcagt atttatagct ctggttttta ttggctacat cacttttttt acatcaaatc
      121 attttgctaa acctgctcca tatacatttg agattaaaga tggtgaaaca ttttcaagtg
      181 tcactaaaag actgtttaac gaaggcatca ttccgagcaa attcaatttt aaagcagctg
      241 catatattta cgatgctgaa aaaaaaataa aagcagcgcg ttttaaaata aaaaatgatt
      301 taagctattt agatttattg gatttgttag ttaacggtcc ggctgattat ttaagaaaaa
      361 tacgaattaa tgacggacaa actcttaaat ggcttggtgc taaggtaaaa agagatgtta
      421 agatagattc ttcagcgttt gtaaatcttg caaaagataa ggagtttatt aaatcattag
      481 ggttaaatgt ttctacttta gaaggatatt tgttttctaa aacctataag atttatgaaa
      541 attcttctcc tgaagaagca ataaaaatat tttacgatag cttcaaagaa ttttttaatg
      601 attcattaaa gcaaagagca aagcaaatcg gattaacagt tcaccaggtt ttaactctcg
      661 cgtccattat taaaggtgaa acagatcatg ttgaagagat gccgactata gcaggagttt
      721 atcataacag gttaagaatt ggtatgcgat tgcaggctga tcctacaata caatatttgc
      781 tgccgggcgg ttggagaaga ttaacttata aagatttaca gattgaatca ccttataata
      841 cctataaata ttccggatta ccgccgggac caattaacaa tccagggaaa aatgcaatta
      901 tggctgtgct atatccggaa aagaataatt atttgtattt tgtagccaac ggaaatggcg
      961 gtcataattt tgccagatca tattcagatc atttaaagaa tgttgctaag tacagaagat
     1021 ggttacaaac acaaaacaat caataaaaat attttttatt tatttctcaa tatttttctt
     1081 agccggatgc gcaaatcaac ttcctccggg tgggggagaa attgatacaa ttcccccggt
     1141 tattatctcc tcttatcctg aaaatggtac aacaaatttt aaagacgatt atatcgagct
     1201 agaattttct gagtatgttg ataaaagatc ttttaaagat gctttgttta tttctcctgc
     1261 aatagacggg cagccggaaa tcagctggag tggtaaatct gtagagataa attttccaaa
     1321 gggattaaag gacagtttaa cttatgttgt aacaattggg actgatgttg ttgatgtaaa
     1381 taataaaaat cgaatggcta attcattttc attttcgttt gcaacaggca gcttgattga
     1441 taagcgaagt attgatggca aagtatatgg taaaaatgtg gaaggtactt taatttttgc
     1501 ttataaatat tctgatgaca ctacaaaata tctgtataag aaaccagatt atctttcaca
     1561 aataggtaag gacggcagct ataaacttac tggtttagct gaagctgttt atcttgtgtt
     1621 tgcagttaaa gatgaatttc gtgatttact ttatcaggca gatcaggatt ggatcggaat
     1681 gccaagtaaa gaaatttcat taacaaatac tgattcatcc tttacaggtc taaatttttt
     1741 tttaatgaaa gtggatactg ttaaaccaag aatacttggt actgtaatga ctgataagaa
     1801 tcattttgtc atcactctta gtgaagagtg tgattcctca acttatctgg caaataattt
     1861 tcaaataatt gattcgacct ctaatcaaat aattccggtt gaatattcgt accataacaa
     1921 atccaa
//
LOCUS       DASWBY010000233         6789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_10325, whole genome shotgun sequence.
ACCESSION   DASWBY010000233 DASWBY010000000
VERSION     DASWBY010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10325"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VF870_04220"
     CDS             <1..961
                     /locus_tag="VF870_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9251421.1"
                     /translation="IGKRSIFVVLGYGISAIVKPLPGIFQNIPTVLISRITDRVGKGI
                     RTAPRDALLASYSDGNSGAVFGFHRGMDTLGAAIGPVAALILLNFYPNNFQLIFLVAF
                     IPSVIEVSFTLLVKDKQKSVNIKSKIKYLWFWKSAPKEYKTVLVLITIFSLLNSSDVF
                     LILKSQNISKSNSLAIFGYVFYNIIYAASSYPLGGLSDKLGKQKVFSFGLIIFSAVYF
                     GFAFVDNIDFIWVFFALYGIYAASTEGVSKAWISDLITNEQRGSAIGLLTMFSSFSVL
                     IGSFLTGVLWDKFGSSVPFIISASSSLIVSIIIITLNPKKTHR"
     gene            1072..1764
                     /gene="queC"
                     /locus_tag="VF870_04225"
     CDS             1072..1764
                     /gene="queC"
                     /locus_tag="VF870_04225"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX9251422.1"
                     /translation="MHSNKKNIAVIALSGGMDSCVTASIANLDYELAFAHINYGQRTV
                     NRELKAFDEIADHYRVKKRLIIDYTHLAKIGGSSLTDKNIEVTKADLENQNIPSSYVP
                     FRNANILSACVSWAEVLNASAVFIGAVYEDSSGYPDCRPDFFSAFEKMIDLGTKPETK
                     IKIVTPIINLSKAEIVRKGIELDAPLHLTWSCYQNEDEACGVCDSCALRLRGFQQAGF
                     EDPIKYKIRPKY"
     gene            1786..2199
                     /gene="queF"
                     /locus_tag="VF870_04230"
     CDS             1786..2199
                     /gene="queF"
                     /locus_tag="VF870_04230"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX9251423.1"
                     /translation="MNDKTKLLETFENKYPNRDYTVTHIAPEFTSLCPKTGQPDFATI
                     TLEYIPDKLCVELKSYKFYLQSYRNDGIFFESVTNKILDDLVAVTQPRWMKITAEFNT
                     RGGISSVIEAEYEDEDYWYDDEGYDEDFDDDFLKN"
     gene            2212..4785
                     /locus_tag="VF870_04235"
     CDS             2212..4785
                     /locus_tag="VF870_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9251424.1"
                     /translation="MLHLLEIKKEFLKERENLFASFNGKSDAFEFSKNYSLLLEDTIR
                     IIIGDKKISFALASAGSFSRRELSPYSDIDLMIISDSIEKDDEEIKALVTNLWDGGIE
                     ASHTVRETSDIQKYLTEDLHTFTQFFETRFISGDAILHNKWNTVLLDSINDDSKKLLI
                     TNFINDVKQRHDKYGGSPKMLEPNVKMSAGGLRDFQSIEWMMMIASKQLLNSQHELTE
                     AEIFINYLKKDKLATPAECKRLLESYKLILSIRHLLHITTKSKTDRFEFTNQLALAKI
                     LGYEETDLMGFMKNYFNATNVVFRVTHTLIKKYKVEYVNPVPDSLSYDLDEDFYIKNK
                     VIYQRKKEPLTLSDIFRVFYYRVSQNAGFDDHLRTKIIEATENAEENNWSQPESSVFF
                     REILKFPRHVGSTLAIMNELGVLGAFLPEFGDLNGFMQHGVYHCYTADEHTLITIRNL
                     EKLANQNSTYSQLYNSIKDKEILNLALLLHDIAKPIDISGHEIIGAEMASSIMMRLGY
                     GEGEIDKVCFLVKNHLMMEQIAFRRNLNDPETLNSFASRFNSIEELDLLYLITYADLS
                     AVNPAVWTSWKAELLSELYRKSKAMIQDKISGEELLYSTIYSAPKDILKHSDKITENH
                     VQDHFDSLHDDMGYVNHFSDEEIAQHVEEILKGDSVSVLFKELDDFTNVTVITKDFPS
                     LLSKLCGVFAINDANIHSAKIFTRKDGIVIDTFLVTDFRTHKRVDTNKYEKIKEDFYS
                     VINGFLELHQEVAKLKSRWWRIENKLFKRSGQIKITFEKHDRYTILDVHSPDRLGFLY
                     HVTNKMNKLGLIIYFAKISTKGDDIVDSFYVLDRNGHKVSQNDYQFIISELTDTITQI
                     L"
     gene            4803..5621
                     /gene="murI"
                     /locus_tag="VF870_04240"
     CDS             4803..5621
                     /gene="murI"
                     /locus_tag="VF870_04240"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX9251425.1"
                     /translation="MEKEKPIGVFDSGIGGLTVVKRLSSVLPRESIVYFGDTARVPYG
                     SKSNATVIDYSIQDTNFLLKKNVKAVVVACNTASSIAIPELRKKFDVPVIGVILPGAD
                     MAYKETRNGRIGVIGTLATIANKAYSNAIKEIDNTIEVFEKACPLFVPIAEEGWTHHR
                     ATYEIAEEYLKELREKNIDTLVLGCTHYPILADVIQEVIGKDVKLIDSGIATAEVVRN
                     EINRIGFETNSAVPGNLEVYVSDIPTTFKIVAELFLGRNINDVVKVDIEELHKI"
     gene            complement(5728..6699)
                     /locus_tag="VF870_04245"
     CDS             complement(5728..6699)
                     /locus_tag="VF870_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX9251426.1"
                     /translation="MKKILVVFTGGTFSMMIDEKTGGAIPRYSGTELLGMIPEATSLA
                     EIECFDFGKYPGPHVTPELMLDLSKKINAKIKSNRYDGVIVTHGTDTLEETAYFLDLT
                     ITTEIPIVVIGAMKNSSEPDWDGPKNLIDALHICLNENSKNLGVLVCLNGEINAASEV
                     TKIYSDSVESFKSLDFGTLGFVQNGRVIFNRLPRHLETIETEKINKNVDLLTVYAGMD
                     EKFFKFSVDSKVDGIIVEALGVGNVPPPAFEGIKYAVEKGIPVVLTSRCPAGETDYIY
                     SYPGAGKHLHDLGVIFSDYLNGQKARIKLMIVLGLTSKQNEIRKYFE"
BASE COUNT         2214 a         1149 c         1195 g         2231 t
ORIGIN      
        1 aatcgggaag cgctcgattt ttgttgtttt aggttatgga atttctgcta tcgtaaaacc
       61 attaccagga atttttcaaa acattcccac agttttaatt tcaagaataa cagacagagt
      121 tgggaaagga attagaacag ctccgcgtga tgcgctgctt gcaagttata gtgacggaaa
      181 ttccggggca gtatttggat tccatcgcgg gatggataca ctaggtgctg caattggacc
      241 ggtcgctgct ttaattttat taaactttta tccaaataat tttcaactga tatttcttgt
      301 tgcatttatt ccatcagtaa ttgaggtatc atttacactt ttagtaaagg acaaacaaaa
      361 atctgtaaac attaaaagta aaataaaata cctgtggttt tggaaatccg caccgaaaga
      421 atataaaacg gttttagttt taattacaat tttttcactt ttaaatagca gcgatgtttt
      481 cctgatctta aaatcacaaa atatatctaa gtccaattct ttagcaattt ttggatacgt
      541 gttttacaat ataatttatg cagcttcatc ttatccgctt ggaggattat ctgataaact
      601 tggaaagcaa aaagtttttt catttggatt gataattttt tctgcggttt attttggatt
      661 tgcttttgta gataacatcg attttatatg ggtgtttttt gcattgtacg gaatttatgc
      721 cgcatcgaca gaaggcgttt ccaaagcctg gatttcggat ttaattacaa atgaacaacg
      781 cggatcagcg attggtttac ttacaatgtt ttcaagtttt tctgttttga ttggttcatt
      841 tctcacaggt gttttgtggg ataaatttgg ttcatcggtt ccatttatca tttccgcttc
      901 ctctagtctg attgtttcta ttattatcat tactttaaat cctaaaaaaa cacaccgata
      961 atcaatactt agaatgtttt ttgaaattta acttctgatt tttgaattac tcaatctatt
     1021 cccttttttg tattttgtga tcaatatttt caaatagtta aacttttttt aatgcactca
     1081 aacaaaaaaa atattgcagt aattgcatta agcggcggaa tggatagttg tgttactgct
     1141 tcaattgcaa atctcgatta cgaacttgct tttgcgcata ttaattacgg acagcgcact
     1201 gtaaaccgtg aattaaaagc ctttgatgaa attgctgatc attacagagt aaagaaaaga
     1261 ttaattattg attacacaca tcttgcaaag atcggcggtt catcccttac ggataaaaat
     1321 attgaagtaa caaaagctga tttagaaaat caaaacatcc catcatctta tgttccgttc
     1381 agaaatgcaa acatactatc tgcttgtgta agctgggctg aagttttaaa tgcaagtgcg
     1441 gtttttattg gagcggttta tgaggattcc tccggttacc cggattgcag acctgatttc
     1501 ttttcagctt tcgaaaagat gatagatctt ggaacaaaac cggaaacaaa aattaaaatt
     1561 gttactccga ttataaactt atccaaagca gaaattgtta ggaagggaat tgaacttgat
     1621 gcaccacttc atttgacttg gagctgctat caaaatgaag acgaagcttg cggtgtttgt
     1681 gacagctgtg ctttacgtct gcgcggtttt caacaagctg gatttgaaga tccaattaaa
     1741 tacaaaatta gaccaaagta ttaattaaga aagacaatca aactgatgaa cgataaaaca
     1801 aaacttttag aaacttttga aaacaaatat ccaaacagag attatactgt aacgcatatt
     1861 gctccggagt ttacctccct ttgccccaaa accggtcagc cggattttgc aacaattact
     1921 cttgagtata ttcccgataa actttgcgtg gaattaaaat catacaagtt ctatttgcaa
     1981 tcttaccgga acgatggaat tttttttgaa agtgttacca ataaaattct tgatgatctg
     2041 gttgcggtta ctcaaccaag atggatgaaa atcactgctg aatttaatac tcgcggtggt
     2101 atttcctccg ttattgaagc cgaatatgaa gatgaagatt actggtatga tgatgaaggt
     2161 tatgacgaag attttgatga tgattttctt aaaaactagt taaaattatt tatgctgcac
     2221 ttacttgaaa taaaaaaaga gtttcttaaa gaaagagaaa atctgtttgc ctcattcaat
     2281 ggtaaatcag atgcgtttga attcagtaaa aactatagtc tgcttcttga agatacaata
     2341 agaattataa ttggcgataa gaaaatctct tttgcattag cttctgctgg tagttttagc
     2401 aggcgtgaac tctcgccata ctccgatata gatttgatga tcatttctga ttctattgaa
     2461 aaagacgatg aagaaataaa agcacttgta acaaatttat gggatggtgg aattgaagct
     2521 tcacatactg ttcgcgaaac atctgatatc caaaaatatt taacggaaga tcttcacacc
     2581 tttacacaat tttttgaaac ccgttttatt tccggtgatg caattcttca taataaatgg
     2641 aacactgttt tacttgattc aattaatgat gattcaaaaa aattactcat caccaatttt
     2701 attaatgatg taaaacagcg ccacgataaa tacggtggtt caccaaagat gcttgagcca
     2761 aatgttaaaa tgtctgcagg cggtttacgt gattttcaat ccatcgaatg gatgatgatg
     2821 attgcaagca agcagttact caactctcag cacgaactta ctgaagcaga aatttttatc
     2881 aactatctta agaaagataa actagcaact cctgcagagt gcaaacgatt acttgaaagc
     2941 tacaaattaa tcctttccat cagacatctt cttcacatta caacaaaatc aaaaacagat
     3001 agatttgaat ttacaaacca gttagcgctc gctaaaattt tgggatatga agagaccgat
     3061 ctaatggggt ttatgaaaaa ctactttaat gcaacaaatg tagtctttcg tgtaacccat
     3121 actttgatta aaaaatacaa agttgagtac gttaatcctg ttcccgattc cctttcgtac
     3181 gatcttgatg aggattttta tataaagaat aaagttattt accagagaaa aaaagaaccg
     3241 ttaactcttt cggatatctt cagagttttt tattaccggg tttctcaaaa tgcaggattt
     3301 gatgatcacc ttcgcacaaa aattattgaa gcaacagaaa atgctgagga aaataattgg
     3361 tctcaaccgg aatcttcagt tttctttaga gagatcttaa agtttcctcg ccatgtcgga
     3421 tcaacgcttg caattatgaa tgagcttggt gtgctcgggg cttttcttcc ggagtttggc
     3481 gatcttaatg gatttatgca gcatggtgtt tatcactgct acactgcgga tgagcacaca
     3541 ttaattacta ttcgcaactt agaaaaactt gcaaatcaaa attcaacata cagtcagtta
     3601 tataattcca ttaaagacaa agaaatttta aatctggctt tgctacttca cgatattgct
     3661 aagccaattg atatttccgg acacgaaata atcggggcag aaatggcatc atctattatg
     3721 atgaggcttg gatatggaga aggagagatt gataaagttt gctttctcgt aaaaaatcat
     3781 ttgatgatgg agcagattgc attccgcaga aatctaaatg atccggaaac attaaactct
     3841 tttgcctcaa ggttcaattc gattgaagaa cttgatttgc tttatcttat aacttatgct
     3901 gatctttctg ctgtaaaccc tgcagtatgg acatcctgga aagccgaatt actttctgaa
     3961 ctttatcgca aaagcaaagc aatgattcag gataaaattt ccggtgaaga gcttctctac
     4021 tcgacaattt attcagcacc aaaagatatt ttaaaacatt cagataagat tacagaaaat
     4081 catgtgcagg atcattttga ttctttacac gatgatatgg gttatgtaaa tcatttttct
     4141 gatgaagaaa ttgctcagca tgtagaagaa attttaaaag gtgatagtgt ctcggtttta
     4201 tttaaggagc ttgatgattt tacgaatgtt actgtaatta caaaagattt tccttcattg
     4261 ctttcaaaac tttgcggtgt ttttgctatt aatgatgcta acattcactc ggcaaaaata
     4321 tttacacgta aagatggaat cgttatcgac acctttctcg ttactgattt caggactcac
     4381 aaacgagttg acacaaacaa gtacgaaaaa attaaagaag atttttattc agttataaat
     4441 ggatttttag aacttcacca ggaagttgca aaattaaaat cacgctggtg gcgaatcgaa
     4501 aacaaattat ttaaacgttc cggacaaata aaaattactt ttgagaaaca tgatcgctac
     4561 acgattttag atgttcattc acctgataga ttaggatttt tatatcacgt aacaaataaa
     4621 atgaataagc ttggattgat aatttacttc gcaaaaattt ccaccaaagg tgatgatatt
     4681 gtggattcat tttatgtcct tgacagaaac ggacacaaag tttcacagaa tgattatcag
     4741 tttataattt ctgaattaac ggacactatt acacaaattt tgtaggtgct cttttgaatt
     4801 ttatggaaaa agaaaaaccg atcggagttt ttgattccgg tattggcggc ttaacagttg
     4861 ttaaaagatt atcctctgtt ttgccgagag aaagtatagt ttactttggt gatactgcgc
     4921 gagtacctta cggttcaaaa tccaacgcaa ccgttattga ttactcaatt caggatacaa
     4981 attttttgct taagaaaaat gttaaagcag ttgttgttgc ctgcaatact gcatcatcaa
     5041 ttgcaatacc tgagttaaga aaaaagtttg atgttccggt tatcggcgta attttacctg
     5101 gcgcagatat ggcttacaaa gaaacaagaa atgggagaat tggcgttatt ggaactcttg
     5161 ctacaattgc aaacaaagct tactcaaatg ctataaaaga aattgataat acgattgagg
     5221 tttttgaaaa agcttgtccg ttatttgttc caattgcgga agaaggctgg actcatcatc
     5281 gtgcaactta tgaaattgca gaagaatatc ttaaagagct aagagaaaaa aatattgata
     5341 cacttgtgct tggatgcaca cactatccaa tacttgcaga tgtaattcaa gaagtaattg
     5401 gcaaggacgt taagttgatt gactcaggaa ttgcaactgc agaagttgta cgaaatgaaa
     5461 ttaacaggat tggctttgaa acaaattcag cagtacccgg gaatttagaa gtttatgtta
     5521 gtgatattcc cacaacattt aagattgttg cggaactttt tcttggcaga aatattaacg
     5581 atgttgtaaa ggttgatatt gaagagctcc ataaaatctg atattgaatc gtcatcacga
     5641 ggatggcaaa gccagacgaa gtgatctgta gtttttctgc agattgcttt accgactaaa
     5701 gccggattcg caatgacaaa caatttatca ctcaaaatac tttcttatct cgttctgttt
     5761 actggttaat ccgagtacaa tcatcaattt tattcttgct ttttgcccgt taagataatc
     5821 tgaaaaaatt actccaagat catgcaagtg ttttccggca ccgggataac tataaatgta
     5881 atctgtttct ccggcaggac agcgggaagt cagcacaaca gggatcccct tttcaactgc
     5941 atatttaatt ccttcaaatg ctggcggagg aacattgcca acacccaaag cttcaacaat
     6001 aattccatca actttactgt caacagaaaa cttaaaaaac ttttcgtcca ttccggcgta
     6061 aacagtaaga aggtctacgt ttttattaat tttttctgtt tcaattgttt caaggtgtct
     6121 tggcaaacga ttaaaaatta ctcttccgtt ttgcacaaaa ccgagtgttc caaaatctaa
     6181 acttttaaat gactcaacag aatctgaata aatctttgtt acttcgcttg ctgcatttat
     6241 ttctccattc aaacaaacaa gcacaccaag attttttgaa ttttcattta agcaaatgtg
     6301 taaagcatcg ataagatttt tgggcccatc ccaatcaggt tctgagctgt ttttcattgc
     6361 accaataact acaatgggaa tttctgttgt aatggttaaa tctaaaaagt aagcggtttc
     6421 ttcaagcgta tctgttccgt gagttacgat cactccatcg tacctatttg attttatctt
     6481 tgcattaatt ttttttgata ggtccagcat taattcaggg gtaacatgcg gaccaggata
     6541 ttttccaaag tcaaaacatt caatctccgc caaagatgtt gcttcgggaa tcattccgag
     6601 taactcggtt cctgaatatc ttggaatagc accaccggtt ttttcatcaa tcatcatcga
     6661 aaaagtgccg ccggtaaata caactaatat ttttttcatt tatttctcaa ttttttgtga
     6721 ccaaaaatag cttttaactt ctattctaaa aagaaatcta tgctatttat catcataaga
     6781 ttttactga
//
LOCUS       DASWBY010000234         8901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_83208, whole genome shotgun sequence.
ACCESSION   DASWBY010000234 DASWBY010000000
VERSION     DASWBY010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83208"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VF870_04250"
     CDS             <1..263
                     /locus_tag="VF870_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX9251427.1"
                     /translation="HFMGYNYYVFQYSTSLIYATAFAEKIANEGTPAVDNYYKILKGG
                     GSKYAADLIRDAGIDPLSSEPFELTMKKMNKVMDQMEEILNK"
     gene            complement(376..831)
                     /locus_tag="VF870_04255"
     CDS             complement(376..831)
                     /locus_tag="VF870_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251428.1"
                     /translation="MNEVKANKDYNPEMHSAEHILNQTMVRMFNKGRSFSNHIEKKKS
                     KCDYHFDRNLTDEEVETVNKKVNEVIQQDLKVTEGFLHRDEAINNFKLTQLPDDAGNT
                     IRIIKIGDYDACPCSGVHVSSTKEIGKFQIISSSFEDGVLRVRFKLPKE"
     gene            complement(835..1344)
                     /locus_tag="VF870_04260"
     CDS             complement(835..1344)
                     /locus_tag="VF870_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9251429.1"
                     /translation="MDIQEALLNRRSIRKYKNQKISKEDLNTMLKAAMYAPSAMNSQA
                     WQFIVIDDKQILTETIKSIPYAEMLRQSAAAIIVCGASLVEKNESWLLQNCSAAIQNI
                     LLSAHGLGIGSCWIAIHGMDDVCRNIKTQFKLPENIVPVSLISLGYPDEVVTEEERFK
                     QDKIHYNKW"
     gene            1454..2440
                     /locus_tag="VF870_04265"
     CDS             1454..2440
                     /locus_tag="VF870_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9251430.1"
                     /translation="MHFMTQLKDKINKKSKINYFMAGVLFVVGLIFISTNLFFTHNTT
                     SSDDGISDKMFPQDYKIISPKIPENITIFGEGVPLENFEVYERVDREILVNTYWHSAT
                     ILAIKRAARWFPVIEPILKENNIPDDFKYLAVVESNLENVVSPAGATGFWQFIKSATK
                     QYGLEVNDEVDERYDVEKSTVAACKYLNTAYQMFGNWTMSASSYNAGMNGIDKWSGLQ
                     KTTNYWNLVLGSETSRYVSRIIAMKLIMENPSAYGYDLKEEDLYKPLRYKEVELNTSV
                     TDFAEYASSIGVNYKTLKLYNPWLRDTALKNKSGVVYKIKIPEEGSINLIKE"
     gene            complement(2478..2813)
                     /locus_tag="VF870_04270"
     CDS             complement(2478..2813)
                     /locus_tag="VF870_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9251431.1"
                     /translation="MNLESPTTYEGFLEITKSNKAVCFYLSTPECNVCKVLKPKVIEM
                     IENDFTKIDFCYVDLNEAKEISGQLSVFSVPTILVFFEGRETIRASRNVHLKELREQI
                     NRYYKMMFE"
     gene            2944..4821
                     /locus_tag="VF870_04275"
     CDS             2944..4821
                     /locus_tag="VF870_04275"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9251432.1"
                     /translation="MKSFIYTLFVIVFTLSISFGSDLKTPKELKKAYSNQTRSYDGKP
                     GIKYWQNFSDYNIKAEIDPKTRLLTGSEMIVYFNNSPDTIKNIVLKLYQDIYKKGAAR
                     NTELNTESVTDGVKISRFSIDGKTLSLNIDDKVISYQGTNLIIKLSSPLPPKSKKEIE
                     VDWSFVIPKGTDIRIGTYGESTFFVGYWYPKVAVYDDIRGWDMISYDAEHEFYFDYCN
                     MNVEIKVPKGFGVWATGMLQNPGEVLQDEYLKKYNEALTSDKLINIVAKEDSGKEVYK
                     HQNGLTNFKYSAKNISDFAFGISDFYLWDGTSVELEKGKRVACFSAYNPVSDFFSKAA
                     EMAKRSMEFYSEKMPGVIYPYAQITVFNGDGGMEFPMIVNDGQFSNPINDVYVTTHEI
                     AHMYFPFYVGTNESRYAWMDEGMAYFLPWDLQIELSQYDHRTRAAVGFSKWSGNETDI
                     PMILPSILSRDPHLSMMSYYKPAIAYEMLKSALGEDLFAKCLKEFIARWNGKHPTPWD
                     MFYTFEDVTKKDLSWFWNPWFFESHIPDLAIKDLKTENGKVKVLIENVGILPLPVDLT
                     LTLEDGTQTKLTASTSIWEKGEKEVWLDAEVKEKVKSVELLNKNIPDADVSNNKFVNI
                     Y"
     gene            complement(4927..5415)
                     /locus_tag="VF870_04280"
     CDS             complement(4927..5415)
                     /locus_tag="VF870_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9251433.1"
                     /translation="MPIEANQVVTMNFTLKDDTGNVLDSTDAGGPFSYISGTGMVLPK
                     LEEAVNGMIIGTKKQLKLEAKDGYGLYNEDAVQAVGKENFPEDFVLEAGMEYMASNPD
                     GVQMPFIITNVEDETVTIDFNHPLAGKNLNFDLELLDVRDATPEELAHGHVHGAGGHH
                     HH"
     gene            complement(5557..6447)
                     /locus_tag="VF870_04285"
     CDS             complement(5557..6447)
                     /locus_tag="VF870_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX9251434.1"
                     /translation="MKTFIYLIIAFIMLLLSVQAQDVEDTLMIYGRNRFYKTHLPLGY
                     NFQKQYPVVFVFHGGLGNPDLTEKQTGFSKKADEEEFIVIYPYGTGSFDKKLLTWNTW
                     DCCGYADKKNINDVDFIKALLKKIKSEYSIDDKRIYAAGLSNGGMMCYLLACELPEYF
                     AAVAPVSATMFDTVSCNPKNEVSLIIFNSIDDQHIPYNGGVGEKSLVDVEKLPVETVV
                     NMWVKKYDCLLMHKSESSSFHKTTYKNDNGTEIVLYKMLSGGHSWPGGQKIRMFGDTP
                     VKDVSATDLIWEFFKNNPKH"
     gene            complement(6585..6657)
                     /locus_tag="VF870_04290"
     tRNA            complement(6585..6657)
                     /locus_tag="VF870_04290"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6622..6624),aa:Lys,seq:ctt)
     gene            complement(7094..7477)
                     /locus_tag="VF870_04295"
     CDS             complement(7094..7477)
                     /locus_tag="VF870_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9251435.1"
                     /translation="MQEIPTLYEGTRTGFEDFTREVIEDILIEKVNFTRATFKEAQEF
                     KDRLVYDILMNNEKIIIDLSSCEYIDSTFLGALVVILKKMAERSGEIKYVIPQPSALY
                     LFKITGLYGVLNLYRTRDEAIESFI"
     gene            complement(7677..8270)
                     /locus_tag="VF870_04300"
     CDS             complement(7677..8270)
                     /locus_tag="VF870_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9251436.1"
                     /translation="MKRNAVFAVLVSVWSTFLLLSFAGCVQEQQKKEMTQSELVERGK
                     LLVKVGGCTDCHTPKVFGPEGPKPDPLRFLSGYPAENPLPKVDLSMIQPGKWVLFTQD
                     ITGAVGPWGASFAANLTPDNETGIGTWQPDMFINAMRTGKHLGAGRPILPPMPWQGVG
                     ELSDEDLNAIFAYLKSIKPVKNKVPEPKSLDQLTSIK"
     gene            8440..8805
                     /locus_tag="VF870_04305"
     CDS             8440..8805
                     /locus_tag="VF870_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251437.1"
                     /translation="MKKFLVFVLLVIAGYFAYDNFIKEKEVYEIKDSYNKTRALVDID
                     APALSPTDYAHYQGSIKNISDETLNNIVIEYLIDAQPSTAKIDKLEPGEEKNFTTNPV
                     MLRNMDPAHYLKSVNYDGK"
BASE COUNT         2995 a         1471 c         1505 g         2930 t
ORIGIN      
        1 ctcattttat gggatataat tattatgttt tccaatattc aacttcgctt atctatgcaa
       61 ctgcatttgc agaaaagata gcaaatgaag gaacacctgc tgtggataat tattataaaa
      121 tattaaaagg cggcggttca aaatatgccg ctgatttgat cagagatgcg ggaattgatc
      181 cgttatcatc agaaccattt gaattgacga tgaaaaaaat gaataaagta atggatcaaa
      241 tggaagagat attgaacaag taaagccata ttgtgatttg ttatttcgat cccaccagga
      301 gagaaatctg tttaactaag cagccagatt tctctctttg ttcgaaatga cttaaataaa
      361 tggaaattat tagagttatt cttttggcag cttaaatctt actcttaaga ccccgtcttc
      421 aaaacttgat gatatgatct gaaactttcc tatttctttt gtagaagaaa catgaacgcc
      481 gctgcaagga caggcatcat aatctccaat tttaattatg cgaattgtat taccggcatc
      541 atccggtagt tgggtaagtt taaaattatt tattgcttca tcacgatgta gaaatccttc
      601 tgttaccttt aaatcctgct gaataacttc atttactttt ttgtttactg tttcaacctc
      661 ttcatctgtt aagttacgat cgaaatgata atcgcattta gattttttct tttcgatatg
      721 atttgaaaat gatcttcctt tattaaacat tctaaccata gtttgattaa gaatgtgctc
      781 ggcagaatgc atttcggggt tataatcttt atttgcttta acttcattca tacattacca
      841 tttattgtag tgaattttat cttgtttaaa tctttcttcc tcagttacaa cttcatcagg
      901 ataaccaagt gaaattaatg aaaccggaac aatattttcc ggtaacttaa attgtgtttt
      961 tatgtttctg caaacatcat ccattccatg tatcgctatc cagcatgatc caattcctaa
     1021 tccgtgagca gaaagtaaaa tgttttgtat agcggctgaa caattctgca agagccagct
     1081 ttcatttttc tcaactaaag aagctccgca cactataatt gccgcggcag attgtcttaa
     1141 catttcggca tagggaattg atttaattgt ctcagtaagt atttgtttat catcaataac
     1201 aatgaactgc caggcttgtg aattcatagc gcttggggca tacatagcag ctttaagcat
     1261 tgtattcaaa tcttctttac tgattttttg atttttatac tttcttatgc ttcttctgtt
     1321 aagcaatgct tcttgaatat ccatttttcc tcaatagttt ataaaacgtg tacaaagata
     1381 acaacacttc tttttgtctt cgtaattgct ctgtattttt ttattatgca aagcaaatta
     1441 aaaaggattt ataatgcact ttatgacaca attaaaagat aagataaata aaaaatcaaa
     1501 aataaattac ttcatggcgg gagtgttatt tgtcgtggga ttaatattca tctcaacaaa
     1561 tttgtttttc acacataaca caacttcttc cgatgatgga atttctgata aaatgtttcc
     1621 ccaggactat aaaatcattt ctcctaaaat tcccgaaaat attacaattt ttggcgaagg
     1681 agttccatta gaaaactttg aagtgtatga aagagttgat cgtgaaatat tagtaaacac
     1741 atattggcac tccgcaacaa tacttgcaat taaaagagcg gcaagatggt tccctgtaat
     1801 tgaaccaata ttaaaggaaa ataatattcc tgatgacttt aaataccttg cagttgtgga
     1861 aagtaatttg gagaatgttg tttcacctgc tggtgcaaca ggcttctggc aatttataaa
     1921 atcagcaaca aaacagtacg gattagaagt taatgatgaa gtagatgaaa gatatgatgt
     1981 tgaaaaatct actgtagctg cgtgcaaata tctaaacaca gcatatcaaa tgtttggcaa
     2041 ctggacaatg tcggcttcct cttacaatgc ggggatgaac ggaattgata aatggagcgg
     2101 tttacaaaaa accactaatt actggaacct tgttcttggt tcagaaactt cccgctatgt
     2161 ttcaagaata attgcaatga aacttattat ggaaaatcct tctgcgtacg gttatgattt
     2221 aaaagaagaa gatttataca aaccacttag gtataaagaa gttgaactaa atacatctgt
     2281 aacagatttt gcagaatatg catcttcaat cggagtaaat tataaaactc ttaagcttta
     2341 taatccctgg ctgcgcgata ccgcattaaa aaataaaagc ggtgttgttt ataaaattaa
     2401 gattccggaa gaaggaagta taaatttaat taaggaatga tgagcgaaat gatggagtaa
     2461 tttataaaca catcaatcta ttcgaacatc attttataat atctattaat ttgttcgcgc
     2521 aattctttaa gatgtacatt gcgcgaagca cgaattgttt ctctgccttc aaaaaaaaca
     2581 agtatagttg gtacggaaaa aacagaaagc tgtccggaaa tttcttttgc ttcgttcaaa
     2641 tcaacataac aaaaatctat tttcgtaaaa tcattttcaa tcatttctat tactttgggt
     2701 tttaacactt tgcaaacatt acattcaggt gtgcttaagt aaaagcagac agctttgttt
     2761 gatttagtaa tctcaagaaa gccttcataa gtagtcggtg attctaaatt cattctaatt
     2821 ttgtacttaa ttgttaatag aaattttatt taaccgttca tcaaatattt ctttttactg
     2881 aacagagtgt tacaaaaatt tacaatgaaa tttagcaatc aaacgcacca aggaaaaacg
     2941 attatgaagt catttattta cacattattt gttattgttt tcacgctatc aatttctttt
     3001 ggctccgatc ttaaaacacc taaagagtta aagaaagcat actcaaatca aacaagatct
     3061 tatgatggaa aaccgggaat aaagtactgg caaaactttt ctgattataa catcaaagca
     3121 gaaattgatc cgaaaacaag attgcttacc ggcagtgaaa tgattgttta ctttaataat
     3181 agtccggaca caataaaaaa tattgtgtta aaactttatc aggatattta taaaaaaggt
     3241 gcggcaagaa atacagaact gaataccgaa agtgtgactg atggagtaaa gattagcaga
     3301 tttagcattg atggtaaaac attaagctta aatattgatg ataaagttat ttcttaccag
     3361 gggactaatc ttataataaa actttcatct ccgcttcctc cgaaatctaa aaaagaaatt
     3421 gaagttgact ggagttttgt aattccaaaa ggaacagata taagaatcgg gacctatggc
     3481 gaatcaactt tttttgttgg ttactggtac cccaaagttg cggtgtatga tgacattcgc
     3541 ggatgggata tgatcagcta tgatgctgaa cacgagtttt atttcgatta ctgcaatatg
     3601 aatgttgaaa taaaagttcc taaaggcttt ggtgtttggg cgaccggaat gctgcaaaat
     3661 ccaggcgaag tattacaaga tgagtattta aaaaaatata atgaggcact tacttcagat
     3721 aaacttatta atatcgttgc aaaagaagat agcggcaaag aagtgtacaa acaccagaat
     3781 ggattgacaa actttaagta ctcggcaaaa aatatctctg attttgcttt tggtattagt
     3841 gatttttatt tgtgggatgg aacaagtgtt gaattagaaa aagggaaacg agttgcttgc
     3901 ttttcagctt acaacccggt ctcagatttc ttttctaaag ctgcagaaat ggctaaaagg
     3961 tcaatggagt tttattctga aaaaatgccc ggagtaattt atccatatgc gcaaataacg
     4021 gtttttaacg gagatggtgg aatggagttt ccaatgattg taaatgacgg acaattttct
     4081 aacccgatta atgatgttta cgtaaccaca cacgaaatcg ctcatatgta ttttccattt
     4141 tatgttggaa cgaatgaaag ccgttatgcg tggatggatg agggaatggc atattttctt
     4201 ccatgggatt tgcagattga gctttcacag tatgatcata gaacccgtgc agcagttgga
     4261 ttttctaaat ggtcgggaaa tgaaacggat attccaatga tacttccatc aatactttca
     4321 cgcgatccgc atctaagtat gatgagttat tacaagccgg caattgctta tgaaatgctg
     4381 aaatctgctt taggggaaga tctatttgca aaatgtttaa aagaatttat tgcaagatgg
     4441 aatggcaagc atccaactcc ctgggatatg ttttatacat ttgaagatgt gacgaaaaaa
     4501 gatttaagct ggttttggaa tccatggttt tttgaatcgc atatacctga tcttgcaatt
     4561 aaagatttaa aaactgaaaa tggaaaagtt aaagtattaa ttgaaaatgt aggcatattg
     4621 cccttgccag ttgatttaac attaactctt gaagatggaa cacaaacaaa attaacagca
     4681 tcaacgtcaa tctgggaaaa gggggagaaa gaagtatggc tggacgcaga ggttaaagaa
     4741 aaagtaaaat cggttgagtt gttaaataaa aatattccgg atgctgatgt tagcaataat
     4801 aagtttgtta atatttattg atcgttagta gtataatgtt tgggtcatac ttcgactgtg
     4861 ctcagtgtga cataaaaaag aggattgaaa aaattcaatc ctcttttttt gattgatcat
     4921 tcttaattaa tgatggtgat gtccgccagc tccgtgaaca tgtccgtgag caagttcttc
     4981 aggagttgca tcccttacat caagtagttc taaatcaaaa tttaagttct tgccagccag
     5041 cggatgatta aaatcaatgg tcactgtttc atcctcaaca ttagtaatta taaacggcat
     5101 ttgaactcca tcaggattac tggccatata ttccattccg gcttctaaaa caaaatcctc
     5161 tggaaagttt tcttttccaa cggcctgaac agcgtcttca ttatataatc cataaccatc
     5221 tttagcttca agctttagct gtttctttgt cccgattatc attccgttta cagcttcttc
     5281 aagttttggc aaaaccattc cggttcctga aatatatgaa aatggtccgc cagcatccgt
     5341 ggaatctaaa acattaccag tatcgtcttt taatgtaaaa ttcatcgtaa caacttggtt
     5401 tgcttctatt ggcattgtgc ttccttatta atattaattg atgtttaata ataaataaag
     5461 tgagttaaaa ttaaaaagca aaatcaggat tgagtgatta aagcaaccct tagagatctt
     5521 tagcaagaaa atcgagttat gaataaatta aattacttag tgtttgggat tattcttgaa
     5581 aaattcccag atcaaatctg ttgcagaaac gtctttaact ggcgtatcgc caaacattct
     5641 gatcttttgt ccgccaggcc aggaatgacc gccgcttaac attttgtaca aaacaatttc
     5701 ggttccgtta tcatttttgt acgtggtttt atgaaaggat gaactttctg atttgtgcat
     5761 caataagcag tcatattttt ttacccacat gttaacaact gtttcaacag gaagtttttc
     5821 aacatcaact aaagactttt cgcccactcc gccattgtaa ggaatatgct gatcatcaat
     5881 actattaaat attatcagcg agacctcatt tttaggattg caagaaactg tatcaaacat
     5941 agttgccgaa acaggggcaa ctgccgcaaa atattccggc aattcgcaag caagcagata
     6001 gcacatcatt ccgccgtttg agagacccgc agcataaatt cttttatcat caatactata
     6061 ttccgattta atttttttta acaatgcttt aataaaatca acatcattaa tgtttttttt
     6121 atcggcatat ccacagcagt cccaggtatt ccacgttaaa agttttttat cgaaggaacc
     6181 ggttccgtaa ggatagataa caatgaattc ttcttcatct gcctttttag aaaagccggt
     6241 ttgtttttct gttaagtccg gatttcctaa tccaccatga aaaacaaaaa ccacaggata
     6301 ttgtttttga aaattatatc caagcggcag atgggtttta taaaatctat tacggccata
     6361 aatcattaat gtatcttcaa catcctgtgc ctgaacagaa agcagaagca ttatgaacgc
     6421 tattattaag tatataaaag ttttcaagat cattttctcc gatagtaaaa ggaataatga
     6481 tttattcttt caaaacctgt gcaattaaaa aattgctgta aaagtaaaaa accctcgttt
     6541 taagagggta tttttacata aaggatgtta aactttcacg ttagtacacc cggggggact
     6601 cgaacccccg accaatagat taagagtcta ctgctctacc aactgagcta cggatgcgtt
     6661 gtaaaaataa ctaaaagcgc atcttttaca aagtttggag taggttttga aaccactttt
     6721 tgtttttaaa cttaacgttt tactgaacac tctctagtct ttcgttcttg atgaaaggag
     6781 ttgtaaaatg tctgttttta agcgagattc taaatatcat cggtaatgtt ttgatgaagg
     6841 ggagaaacaa aataaaaaag attaatggga aagagtggtt aagatacttt aaaaataaaa
     6901 agaccatctt actgacggtc aataaaatct atagagctat aaatttaact taatggaaca
     6961 atttttttat ttctctcttt tcttcattaa cagctatctt ccataatgag tagcaaaatg
     7021 ctactatcac aataaagagt gtggccaaaa ttattgcttc catgtaatta tcttttatta
     7081 attattaaat aaactaaata aaactttcaa ttgcttcatc tctggttcta tataaattaa
     7141 gcacaccata taaaccggtt atcttgaaaa gatataatgc tgatggctgc ggaataacgt
     7201 atttaatttc tccacttcgc tcagccattt tctttagaat cacaaccaat gctccaagaa
     7261 aagttgaatc gatatattca caactgctaa gatcaattat tattttttca ttgttcatta
     7321 gaatgtcata aacaagccta tccttaaact cctgggcttc tttaaaagta gctctggtaa
     7381 agtttacctt ttcaattaag atatcttcaa tgacttctct tgtaaaatct tcaaagcctg
     7441 ttcttgttcc ctcatacaat gttgggattt cttgcatcaa tttttccaat aaaaatttat
     7501 aaaacttttt aaggttctat acaaataaaa ggccagacga aaaaaggctg attattgatt
     7561 ataaataaat aattttaaca ataaagttaa tcccaggtga tttagagaga ttatattatg
     7621 tttggaaatg aagcaaaaaa gaatccctgc tcaaaatgtg gcagggattc ataaaatcat
     7681 tttatagatg ttaactgatc taatgatttt ggttcaggta ctttattttt tactggctta
     7741 atggatttta gatatgcaaa tattgcattt aaatcctcat ctgaaagttc acctactcct
     7801 tgccacggca ttggcggaag tattggtcta ccagctccaa gatgtttacc cgttctcatt
     7861 gcattgataa acatatcagg ttgccaggtt ccaattccgg tttcattgtc cggggttaga
     7921 tttgctgcaa atgaagctcc ccacggacca actgcaccgg taatatcctg agtgaataac
     7981 acccatttcc cgggctgaat catactgagg tcaactttgg gtaatggatt ttcggctgga
     8041 taaccggata aaaatcttaa tggatctggt ttaggtcctt ccggtccaaa taccttcgga
     8101 gtatgacaat cagtacatcc tccaactttt acaagtaatt ttcctctttc cactaactct
     8161 gattgagtca tttctttctt ttgctgttct tgaacacatc ctgcaaaaga taacagtaaa
     8221 aatgtgctcc aaacggaaac taaaactgca aaaaccgcat ttcttttcat tttgcctcca
     8281 aataattgat tattgataat ggtgtacttt ttgttaaaaa agcaggacaa attttgcaac
     8341 gaagattgct cgttaaaaat aatttattat aggcggttat ttttaattat caatagttgt
     8401 aaaatgtaaa ccaaaaaata tttattaaaa ggaaaaacta tgaaaaagtt tttagttttc
     8461 gttctattag tcatagctgg atattttgct tacgataatt ttattaaaga aaaagaagta
     8521 tacgagatta aagactccta taacaaaacc agagccttag tagatataga tgcccctgca
     8581 ttatcaccga cagattatgc acactatcaa ggtagcataa aaaatataag cgatgaaaca
     8641 ttgaataaca tagttataga atatttaata gatgctcaac catccacagc aaaaattgat
     8701 aagcttgaac caggcgaaga aaaaaacttt actaccaatc cagttatgct tagaaatatg
     8761 gatccggctc attacttaaa gagtgttaat tacgacggaa aataattttt gctgcatcat
     8821 tctgataaag caatagaaat atttattcag gattaaaaat tttttgatgc tgatttgaaa
     8881 ctgttgttaa tgttttaaca c
//
LOCUS       DASWBY010000235         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_234216, whole genome shotgun sequence.
ACCESSION   DASWBY010000235 DASWBY010000000
VERSION     DASWBY010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234216"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VF870_04310"
     CDS             <1..644
                     /locus_tag="VF870_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9251438.1"
                     /translation="NIATYEAALDLINCKVDAIKVGIGPGSICTTRVIAGVGVPQITA
                     IAQAYSASKKYGIPLIADGGIKQTGDVPKAIAAGADSVMIGGLFAGVDESPGDKILYE
                     GRSFKSYRGMGSLSAMKTGSKDRYFQDAEDDIKKLVPEGIEGRVPYKGPLEDTVYQLV
                     GGLRASMGYTGNPNIKSLKTKSKFIRITLAGLKESHPHDVILTQESPNYQSHK"
     gene            659..1945
                     /locus_tag="VF870_04315"
     CDS             659..1945
                     /locus_tag="VF870_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9251439.1"
                     /translation="MFKVLVVAYYFPPLGLSGVQRTLKFVKYMKNYNWEPTVITSGNV
                     AYFAHDKSLSKELNDSEVRIIRVSGSSEPNSILSKFGTVKLPSEFLRNIFDKLSQTFF
                     IPDNKKSWSKLAAKKVDEILSTEKFDAIFVTCPPFSAFDEISKIKKNHDVPLFADYRD
                     LWYKSYFAFYPTPFHKIIHKNKEYHALKAADRIIVTNRKIKEKLLNTYPFLTFEDVVI
                     VSHGYDSEDFEKIPAQPKPQNKMVLMYSGIFMVYSSPVFLLKAFKQLTIERPDIAANI
                     ELHFVGFLRKENQKVIRQLKLQNFVKDHGYVNHDESIAKLKSADVLWFMVGRRQNIDA
                     ILPGKVYEYIGARKPILACVPDGAAKMAVEESGAGYICSPDNIDDIKNTILKVYDDNK
                     NNKLPSPSEEVLEKFRRDNLTEVLTKQFQLKLRAEG"
     gene            1945..3228
                     /gene="purD"
                     /locus_tag="VF870_04320"
     CDS             1945..3228
                     /gene="purD"
                     /locus_tag="VF870_04320"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX9251440.1"
                     /translation="MNVLVIGSGGREHALALKIYQSEELKSLYCIPGNPGTETLANNN
                     NINLTDHKAISRFCKENKIDLIVIGPEQPLVDGLSDHLRNEGFTVFGPSANAARIESS
                     KAFAKKIMIDAGVPTAKFVEFTSAQFDEAIKYLESINYPTVIKADGLAAGKGVLICSN
                     KNEAEFAIREIFEHKIFGDSGNKLIIEEFLEGEEASIFAITDGEKFVCLPAAQDHKRI
                     GDGDTGKNTGGMGAYAPAPIITAELSKEIETKIFKPVITQMLKTGNKFIGCLYAGLII
                     TKTGPKVIEFNCRFGDPETQVVLPILEGDFLDLLYSAAAGNINSAAVQYSGGCSVCIV
                     AASKGYPDSYEKGFVIAGLTRIDNDVIVYHAGTKKENEKVVTNGGRVLGVTSVIQNQN
                     LKLAQQKAYEAISKVSFDGMYYRKDIAEKAFKHLK"
     gene            3295..>3610
                     /locus_tag="VF870_04325"
     CDS             3295..>3610
                     /locus_tag="VF870_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX9251441.1"
                     /translation="MIYLHVHTNFSLLQGTIRIDDLINTCEEKNIKSIALTDTNAMNG
                     LIQFVKKAKENEIQPILGTCLTDPEEDKKYVILLAKNNEGYSDICRIITQRKLNDDFS
                     LIK"
BASE COUNT         1254 a          563 c          705 g         1088 t
ORIGIN      
        1 gaaatatagc tacttatgag gctgcacttg atcttataaa ttgtaaggtg gatgcaatca
       61 aagtcggcat cggaccggga tcaatttgta ctactcgtgt aattgccggt gttggtgttc
      121 ctcaaattac tgctattgca caggcttata gtgctagtaa aaaatatgga attccgctta
      181 ttgctgatgg aggtataaag cagacaggtg atgttccaaa agcaatagcc gctggagcag
      241 attctgtaat gattggcggg ctttttgctg gtgttgatga aagtcctggc gataaaatac
      301 tttacgaagg tcgaagcttt aaatcttatc gcgggatggg ctctttaagc gcgatgaaaa
      361 cgggaagtaa agacagatat tttcaagatg ctgaagatga tattaaaaaa ctggtcccgg
      421 aaggcattga gggaagagtt ccttataaag gaccacttga agatacagtt tatcagcttg
      481 ttggaggttt gcgtgcatct atgggttaca cgggaaatcc aaacattaaa tctctaaaaa
      541 caaagagtaa gtttataaga attacattag ctggacttaa agaaagtcac ccgcatgatg
      601 ttattcttac gcaagaatca ccaaattatc aaagtcataa ataggatttg tatttataat
      661 gtttaaagtt cttgttgttg catattattt tccaccttta ggtctcagcg gagttcaaag
      721 aacgttgaag tttgttaagt atatgaagaa ttataactgg gaaccaacag ttatcacttc
      781 tggcaacgtt gcatattttg cccatgataa atcactaagt aaagaattaa acgactccga
      841 agttcgtatt ataagagttt caggaagcag tgagcctaat tcaattcttt caaagtttgg
      901 aacagttaaa ctgccaagcg aatttttgag aaatatattt gataaactga gtcaaacatt
      961 ttttattccg gataataaaa aatcatggtc taaacttgct gcaaaaaaag tagatgagat
     1021 tctgtcaaca gaaaagtttg atgctatttt tgtaacgtgt ccaccatttt ctgcattcga
     1081 tgaaatatca aaaatcaaga agaaccatga tgttcctctg tttgccgatt acagggacct
     1141 ttggtataaa agttattttg ctttttatcc aactccattt cataaaatca ttcataaaaa
     1201 caaagaatat catgctttaa aggcggcaga tagaataatt gtaacaaatc gaaagattaa
     1261 agagaagctg ctaaatacat atccattttt aacatttgag gatgttgtaa ttgtatcaca
     1321 cgggtatgat tctgaagatt ttgaaaagat tcctgctcaa ccaaaaccac agaacaagat
     1381 ggttttaatg tattccggaa tatttatggt ttacagttct cctgtttttc ttttaaaagc
     1441 atttaagcaa ttaacgattg aacgacctga tatcgcggct aatatcgaat tacattttgt
     1501 tggttttttg agaaaagaaa atcaaaaagt aattcgccaa ttaaagctgc agaattttgt
     1561 taaagatcac ggttatgtaa atcatgatga atcaattgct aaattaaaat cagctgatgt
     1621 attatggttt atggttggca gaagacaaaa catagacgcg atacttccag gcaaagttta
     1681 tgaatatatt ggtgcaagaa agcccattct agcttgtgtt cccgatggtg cagcaaaaat
     1741 ggccgttgaa gaatccggtg ctggctatat ttgcagtcct gacaatatcg atgatattaa
     1801 gaacacaatc cttaaagttt atgatgataa taaaaacaat aaacttcctt ccccatctga
     1861 agaggtttta gaaaagttca gacgagataa tctgactgag gttctaacaa aacaattcca
     1921 acttaaactt cgagccgaag gttgatgaat gttttagtta tcgggtctgg cggcagagag
     1981 catgcccttg ctttaaaaat ctatcaaagc gaagaattaa aatcacttta ttgcattcct
     2041 ggaaatcccg gtacagaaac ccttgcaaat aataataata ttaatttaac tgatcacaaa
     2101 gcaatttcta gattttgcaa agaaaataaa attgatctta ttgtaatagg acctgagcag
     2161 ccattggtcg acggattaag tgatcattta agaaacgaag gctttacagt atttggacca
     2221 tcagcaaatg ctgcacgaat cgaatccagt aaagcatttg caaaaaaaat aatgatcgat
     2281 gccggtgttc caactgctaa atttgttgag ttcacatccg ctcagtttga tgaagcaatt
     2341 aaatatttag aaagtataaa ttatcccaca gtaattaagg ctgatggatt agctgcagga
     2401 aaaggtgttt taatttgcag caacaaaaat gaagctgaat ttgcaatcag agaaatattt
     2461 gaacataaga tttttggtga ttcaggaaat aaattaatta ttgaagaatt tttagaaggg
     2521 gaagaggctt ctatttttgc aattacagat ggtgaaaaat ttgtctgttt gcctgcggca
     2581 caggatcata aaagaattgg tgatggcgat acaggaaaaa acaccggcgg aatgggtgct
     2641 tatgcccctg ctccgataat cacagcagaa ttatccaaag aaattgaaac caaaatattt
     2701 aaacctgtta taactcaaat gcttaaaaca ggaaataaat ttatcggttg tttgtatgca
     2761 ggtttgatca tcactaaaac cggtccaaag gtaattgagt tcaactgcag atttggtgat
     2821 cctgaaacac aagtagtact gccgatttta gaaggtgatt ttcttgatct gctctattct
     2881 gctgctgctg gaaatataaa ttcagctgct gtgcaatatt ctggtggatg ttctgtttgt
     2941 atagtcgccg catccaaagg ttacccggat agttatgaaa agggttttgt gatagcagga
     3001 ttaactagaa ttgataacga tgttattgtt tatcacgcag gcactaaaaa agaaaacgag
     3061 aaagttgtaa ctaatggcgg cagagtgcta ggagtcactt cagtgattca aaatcaaaat
     3121 ttaaaacttg cacagcaaaa agcttatgaa gcaattagca aagtatcgtt tgatggaatg
     3181 tattatcgaa aagatattgc tgagaaagct tttaagcatc taaaataatt ttctgctaca
     3241 atcttttcat agtttgtccc tttcgttaat ttcacatcga ctaaaacgac atacatgatt
     3301 tatctacacg tacatacaaa cttttcatta ctgcaaggta ccataagaat tgatgatctt
     3361 ataaatactt gcgaggaaaa gaatatcaaa tcaatcgcat taacggatac aaatgcaatg
     3421 aatggattga ttcagtttgt aaaaaaagca aaagagaatg agatccaacc aatcctgggt
     3481 acctgtttaa cggatccgga agaagataaa aagtatgtaa tcctgctagc aaaaaacaat
     3541 gagggttatt cagatatttg caggataatc actcaacgaa aactaaatga tgatttttct
     3601 ttaattaaga
//
LOCUS       DASWBY010000236         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_10410, whole genome shotgun sequence.
ACCESSION   DASWBY010000236 DASWBY010000000
VERSION     DASWBY010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10410"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VF870_04330"
     CDS             complement(<1..963)
                     /locus_tag="VF870_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX9251442.1"
                     /translation="MKEPEVTFELAQEHGLIKEEWEKILIILGRTPTFTELGVFSVMW
                     SEHCSYKNSIAQLKTLPRSGGRLLVAAGEENAGLIDIGDDLAVAFKIESHNHPSAVEP
                     YQGAATGVGGIMRDIFTMGARPIASLNSLRFGSLKDARTRFLFDGVVRGIGDYGNSFG
                     VPTVAGEVYFDESYQGNPLVNAMAVGIVNKKHFASATSKGVGNPIMIVGSSTGRDGIH
                     GATFASEEISEKSEAKRPSVQVGDPFTEKLLLEATLEIIKNDWLIGIQDMGAAGISCS
                     TSEMSAKGESGMKINLDKVPLREKGMTAYEIMLSESQERMLCCVK"
     gene            complement(1213..>1647)
                     /locus_tag="VF870_04335"
     CDS             complement(1213..>1647)
                     /locus_tag="VF870_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX9251443.1"
                     /translation="WNSENTNVQYKGLPEKEMLESFWKVALHVDQFITFNGRNFDVPF
                     LMMRSTMLKVKPSKNLMAYRYGDEHIDLLEQFTFYSTTRKFNLDFYCHAFGIESPKSK
                     DISGMEVKTLYEAGRIKDIAVYCSKDIYATYQLFKIWEEYLR"
BASE COUNT          503 a          345 c          264 g          535 t
ORIGIN      
        1 tttaacacag cacaacattc tttcctggct ttcgctcaac ataatttcat aagcagtcat
       61 acccttttcg cgcaaaggaa ctttatcaag attgatcttc attcctgatt cgccttttgc
      121 actcatctca cttgttgaac aagaaattcc tgccgcaccc atatcctgaa ttccaatcag
      181 ccaatcattc ttaataattt ctaaagttgc ttccagtaaa agtttttccg taaacgggtc
      241 accgacttga actgacggac gctttgcttc ggacttttca gaaatttctt ctgaggcaaa
      301 agttgcaccg tgaattccat ctcttcccgt tgatgaacca acaatcataa taggattgcc
      361 aacacctttg gatgtggcag atgcaaaatg ttttttgttt acaattccaa ctgccattgc
      421 attaacaagt ggattgccct gataagattc atcaaagtaa acttctccag caaccgttgg
      481 aacgccaaaa ctatttccgt aatcaccaat tccacgaacg acgccatcaa ataaaaatct
      541 tgtgcgtgca tcttttaaag atccaaaccg aagtgagttc aacgaagcaa tcggacgggc
      601 acccattgta aaaatatctc gcataattcc accaacgcct gttgctgctc cctgatatgg
      661 ctcaaccgcg gaaggatgat tgtgactttc gattttaaat gcaactgcca gatcatcacc
      721 aatgtcgatg agtccggcat tttcttctcc ggcggcaact aataatcttc caccgctgcg
      781 tggtaaagtt tttaattgcg caatagaatt tttataactg caatgttcgc tccacattac
      841 agaaaaaact ccaagctctg taaaagttgg ggtgcgacca agaattatta aaatcttttc
      901 ccattcttct ttaattaaac cgtgctcttg tgctaactca aaagttactt cgggttcttt
      961 catcaatctt ccaattggat ttaaaatagt ttgaaaattt tacaaggaaa tataagggaa
     1021 agagggataa atattttcta ataatttgag atttaagaac ttcagtcacg aaatggttcg
     1081 tgacagaata aatatattat ttcaattcca gcactattaa aacaaacccc tcttgtattc
     1141 tctcctttcg taaaggggag aatttaaaaa gtccttcctt tttgaaaggc aaggatttag
     1201 aaagggttct tttcatctca aatactcttc ccaaatttta aacaattgat aagttgcata
     1261 tatatctttg gagcagtaca cagcaatgtc ttttattctg cctgcttcat agagagtttt
     1321 tacttccatt ccagaaatat cttttgattt tggtgattca attccaaatg cgtggcagta
     1381 aaaatccagg ttaaattttc ttgttgtact gtaaaaagta aattgttcaa gtaaatcaat
     1441 atgttcatca ccatagcgat aagccattaa gtttttgctc ggtttaactt ttaacattgt
     1501 agagcgcatc attaaaaaag ggacgtcaaa atttcttccg ttaaatgtta taaactgatc
     1561 gacgtgtaaa gcaactttcc agaatgattc cagcatttct ttttctggca aacctttgta
     1621 ttgaacattt gtgttttcag aattcca
//
LOCUS       DASWBY010000237         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_83259, whole genome shotgun sequence.
ACCESSION   DASWBY010000237 DASWBY010000000
VERSION     DASWBY010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83259"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VF870_04340"
     CDS             <1..869
                     /locus_tag="VF870_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251444.1"
                     /translation="LSNIMKLLCTIIIICTTTFSAYSQYNPGAKQISLSNSDVALSND
                     VFSLFNNPAGLSQMNWREIGVYYSPAPFGLSELANGYVAYHEPTSIGSFAIGGMSYGF
                     DLYRESKFTLGYSYNYLNKFFAGAAINYQTVSIQNYGSDGAIFLNIGGLAYISNYFRL
                     GFSIQNINRATFGDEDDQIPMIFNTGLSYDVVDELTMNFAVEKDIKYKASFQFGINYD
                     IIEYLSLRVGFSNEPSKYSAGIGINYSMFSLDYAMFTHNDLGLTHQAGVIISFGRELD
                     RNKTIRENLGLE"
     gene            871..>1920
                     /locus_tag="VF870_04345"
     CDS             871..>1920
                     /locus_tag="VF870_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9251445.1"
                     /translation="MRLLSIIFISILLSINLTYAQLDSTSIITEEVLDNILIEPETET
                     DSEELVDILEYLIRNPIDINQVDLFDLSKLPYMDPQSAQKIIDHRNKFGYFFSPSELF
                     AIRDLDKQLIEKILPFIKTSRPENQIESFESIRTESNSFFNKSKVNIRNRVTNDLQNR
                     DGFTSGKYQGSKLKTYNRLLYNYDRNYQAGVLIEKDPGELSYTDFTSFHLLVKDIGVI
                     NNFIAGDYVLEFGQGLALWSPFGFSKGADAVFPVKKRARYLRPYTSAAEYRYYRGSAT
                     TLSYDDFSLTAFYSNNTFDATLDPITGEITSVSQTGFHRFESELNKKAASKSKIIGGV
                     LDYKFAGKMNLGVIYY"
BASE COUNT          646 a          298 c          332 g          644 t
ORIGIN      
        1 aactatcaaa tattatgaaa cttctttgta caattataat aatttgcaca acaactttta
       61 gtgcatattc tcagtataat cctggcgcaa aacaaatctc tctctccaat tctgatgtcg
      121 ctttaagtaa cgatgttttc agtcttttca ataatccagc aggattatca caaatgaact
      181 ggcgagaaat tggggtttat tattctcctg ccccatttgg gctatcagaa ttagctaacg
      241 gttatgtagc ttatcatgag cctacctcaa tcggttcttt tgctattggc ggaatgagtt
      301 acggatttga tctttaccgc gaatccaaat ttaccttggg gtattcatat aattatctta
      361 ataaattttt tgccggtgca gcgattaatt atcaaactgt ttcaattcaa aattatggaa
      421 gtgatggcgc aatattttta aacattggcg gattagctta catttcaaat tacttcagat
      481 tgggattttc aattcaaaat attaaccgag ctacatttgg tgatgaagac gatcaaattc
      541 caatgatctt taatacggga ttatcctacg atgttgtaga cgaattgaca atgaactttg
      601 ctgttgaaaa ggatataaaa tacaaagcct cttttcagtt tggaattaat tatgacataa
      661 ttgaatattt atcattacgc gttggttttt caaatgagcc ttcaaagtac agtgcgggca
      721 tagggataaa ttattcaatg tttagtttgg attatgcaat gttcacccat aatgatttag
      781 gattgacaca tcaggccggt gtaataataa gttttggaag agaactggat cgcaataaaa
      841 ctatcaggga aaatctggga ttggaatagt ttgagattac tatctataat ttttatatcg
      901 attttgttat cgattaacct gacttatgca caattagaca gcacttcaat aattactgaa
      961 gaagttctcg ataacatttt gattgaacca gaaacagaaa ctgattctga ggaactagtt
     1021 gatattttag aatatctaat ccgcaatccc atcgatatta accaggtaga tctgtttgat
     1081 ttatcaaagt taccatatat ggatccgcaa tcagcacaaa agattatcga tcatcgaaat
     1141 aaatttggct attttttttc tcccagcgaa ctttttgcaa ttcgtgatct ggataaacaa
     1201 ttgattgaaa agattttacc atttattaag acatcaagac cggaaaatca gattgagagt
     1261 tttgaatcaa taagaacaga atcaaactca ttttttaata aatcgaaagt aaacattcgc
     1321 aatagagtta ctaatgattt acaaaacaga gatggattca ctagcggcaa atatcaaggt
     1381 tcaaaattaa aaacatataa ccggctatta tataattatg atagaaatta tcaggccgga
     1441 gttttaattg aaaaagatcc cggtgaattg tcttatacag atttcacttc atttcattta
     1501 ctggttaaag atattggagt tattaataat tttattgcag gtgattatgt tttggaattc
     1561 ggacaagggc tagcgctttg gagtccattt ggtttttcta aaggagctga tgctgtattt
     1621 cctgtaaaga aaagagcacg atatcttaga ccatacacaa gcgctgcaga atatagatat
     1681 tacagaggtt ctgcaactac tttatcatat gatgatttta gtttaacagc tttttattca
     1741 aataatactt ttgatgcaac acttgatcca ataaccggtg agattacttc agtcagtcaa
     1801 actgggtttc accgatttga aagtgaacta aacaaaaaag cagcatcaaa atcaaaaata
     1861 attggcggcg ttttagacta taaatttgca ggcaaaatga atcttggagt tatttattat
//
LOCUS       DASWBY010000238         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_496852, whole genome shotgun sequence.
ACCESSION   DASWBY010000238 DASWBY010000000
VERSION     DASWBY010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496852"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VF870_04350"
     CDS             complement(<1..1114)
                     /locus_tag="VF870_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9251446.1"
                     /translation="MEIANLSSFWSLSQEEILKELETNSNGISEDEAQKRIDQYGANR
                     LKAKKKSNSFTLLISQFKSPIILILIFAAILSIFLGDTTDAYIILTIIFISGFLGFWQ
                     ERGAVNAVKKLLEIVQIKSLVLRDGTEREVAIEDIVPGDVIKLTAGSVISGDSFILES
                     NDLFVDESALTGETFPVEKSASTLNKDTVLSQRTNALFMGTHVISGSCKAVIINTGID
                     TEFGKVSERLKLRPAETEFEHGIKKFGFLLLEITLVMIFIIFAFNVYLHKPIVDSFLF
                     SLALAVGLTPQLLPAIISINLSHGAKRMAKEKVIVKKLNSIENFGSMNVLCSDKTGTL
                     TEGKVKIFSLTDISGNKNDKVLLYSQLNANFQTGFVN"
     gene            1239..1934
                     /locus_tag="VF870_04355"
     CDS             1239..1934
                     /locus_tag="VF870_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX9251447.1"
                     /translation="MPSLIHVEKHFNASDTVRDIVIGMSDGLTVPFALAAGLTGAIAA
                     SNLVITAGFAEIAAGSIAMGLGGYLAAKSDYEHYFSENRREESEVKELPALERQEVSD
                     VFKEYGLSDEEIKPILDKFEKNPKNWVDFMMKYELGLEEPDTKRGLKSALTIAVSYIL
                     GGIIPLSPYFFIEDAHNGLAISAIMTMLALLIFGYIKGRFTGSKPIKSAIQTVIIGGI
                     AASVAFLLAKFIS"
BASE COUNT          700 a          348 c          360 g          720 t
ORIGIN      
        1 ggttaacaaa accagtctgg aagtttgcat ttaactggga gtataaaagc actttatcat
       61 ttttattgcc cgaaatatct gtaagagaaa aaatttttac tttgccttca gttaaagtcc
      121 ctgttttatc cgaacaaaga acattcatac tgccgaagtt ttctatagag tttaattttt
      181 ttacgattac tttttctttt gccatccttt tagcgccgtg ggaaagattt atgcttatga
      241 ttgcaggaag caactgcggt gtaagtccaa cagccagggc aagagagaac aaaaatgaat
      301 caactattgg cttatgaaga taaacgttaa acgcaaagat tataaaaatc attaccagcg
      361 taatttccaa taaaaggaac ccaaactttt ttataccgtg ttcaaattca gtttctgctg
      421 gtcgcagttt taatctttct gataccttgc caaactctgt gtcaatacct gtatttatta
      481 taactgcttt acaactgccg ctaattacat gtgtgcccat aaatagagca ttagttcttt
      541 ggcttaatac tgtatcctta tttaaagtgg aagcactttt ttctacgggg aaagtttcac
      601 ccgttagagc cgattcatcc acaaaaagat cattcgattc aagtataaaa ctatcaccgg
      661 aaataacaga acctgctgtt aacttaataa catcaccggg cacaatatct tcaattgcta
      721 cttccctttc tgtaccatca cgcaatacta aacttttaat ttgaacaatc tccagcaatt
      781 ttttaacagc attaacagca cctcgttcct gccagaatcc taaaaacccg cttataaaaa
      841 ttattgttaa gatgatgtat gcatccgttg tgtcaccaag aaaaatagag agtattgctg
      901 caaaaattag aattaggatt atcggacttt taaattggga tataagaagt gtgaatgaat
      961 tagatttctt ttttgctttc agccggtttg caccgtactg atcaattctt ttttgtgctt
     1021 catcttcaga aataccatta ctgtttgttt caagttcttt aagtatttct tcctgtgaga
     1081 gactccaaaa gctggagagg tttgctattt ccatcaattc accttttgtg ataatataat
     1141 ttaattataa aaaatcaatg ttgtattatg ataattacat tctgatctat ttagtatttt
     1201 aagagtaagg aattaaaaca tttaaagcgg agtaaattat gccaagtcta attcacgttg
     1261 aaaaacattt taatgcgagt gatacagtta gagatattgt aattggaatg tctgatggtt
     1321 taacagttcc atttgcactt gccgcaggat taacgggcgc tatagctgca agtaatttag
     1381 tcatcactgc agggtttgct gaaattgctg ctggctcaat tgctatggga cttggtggtt
     1441 atttagctgc aaagagtgat tatgaacatt acttcagtga aaatagacgt gaagaatctg
     1501 aagttaaaga attacctgcg cttgaaagac aagaagtatc agatgtgttt aaagagtacg
     1561 gattatctga tgaagaaata aaaccaatct tagataagtt tgaaaagaat cctaaaaact
     1621 gggtagattt tatgatgaaa tatgaattag gacttgaaga accagataca aaacgcggac
     1681 taaaaagtgc actaacaata gcggtatcat atatacttgg cggcataatc ccgctatcac
     1741 catacttttt tatagaagat gctcataacg gtttagcaat ttctgcaata atgacgatgc
     1801 tggcattatt aatttttgga tacataaaag gaagatttac cggatctaaa ccaattaaaa
     1861 gcgcaattca aactgtgata attggtggta tagcagcgag tgttgcattt cttttagcaa
     1921 aatttattag ttaactatat agtttatata tatcgtattt aattattact taactcttta
     1981 atacttttgt gtaaaatcat taaaattcat attaaatttt ttgaagcata taaaacttac
     2041 taaggattgc acacgatttc tttcgcatta gaacgatcgg gtttcttaat ttaaatactt
     2101 tgaattactt gtgtttctga acttctta
//
LOCUS       DASWBY010000239         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_45649, whole genome shotgun sequence.
ACCESSION   DASWBY010000239 DASWBY010000000
VERSION     DASWBY010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45649"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VF870_04360"
     CDS             complement(<1..1456)
                     /locus_tag="VF870_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9251448.1"
                     /translation="MNILLIVLVLANFTINAQQTNNNKVTDSSTNLIPDISALLRNVE
                     EQRINDSIQKEELKAQLISLKSTDKTQIEELQTKLKEIEDKENRNLEEKKQQIEFLKK
                     STKGYPVVGVLGDTLFHIYSKRGELSAKDRAEILTKKIHELYKDDYFNTDSLKVFETA
                     DNYTIQYGKTIVLSLNETDALWHDKNLSELGNEYKIKIADSIKKAKYENSLSRTLIRT
                     GLVLLVILLTWLLIWLIRLGNRRLMNSINSNKEKWLKKLAYKDYTFLNEEQKYQIILF
                     ITKLLRWFVYIVLIYISLSAIFSIFPITRGWADKLIQYILSPLKSIIIGIWNYLPNLF
                     SILAIYFVMKYFIKFVKYIFTEIDSEKLKVSGFHADWAMPTFSIVKFLLYAFMFVLIF
                     PFLPASDSNIFKGVSVFIGVLFSLGSHSSIANVVAGLVITYMRPFKVGDRIKIGDVTG
                     DVIEKTLLVTRLRTPKNEEVTIPNSSVLSGNTVNY"
     gene            complement(1602..2375)
                     /locus_tag="VF870_04365"
     CDS             complement(1602..2375)
                     /locus_tag="VF870_04365"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HEX9251449.1"
                     /translation="MAKFKLTRSLFLRSVGIGIFIYILNTSWVSAQNSSFEFWPETDI
                     WYRINPSWRLSAFIPITKYHESKYRDINYYLQADYAWGHTKRIYLSRLMDQNREQQMK
                     GWLARGGVMRGWSLGENGDYQENLVLGEIHRRMPLLNNFLLSHRLRPELRWLGENNEF
                     SYRIRYRVMIEKEFKRGNASFVPYVNAEPYWDSRYSAFSRIRLIGGTTVTWRPRFALE
                     GNLTYQYDKHYDTENLYALNLILHLYFESKKVKESENEK"
     gene            complement(2330..>2510)
                     /locus_tag="VF870_04370"
     CDS             complement(2330..>2510)
                     /locus_tag="VF870_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251450.1"
                     /translation="STIFSDRNISLTKDKLNNLPGLSALTVLNIYLHSQDLLFKRLLL
                     VWLNSNSHEVYSYDQ"
BASE COUNT          769 a          481 c          430 g          830 t
ORIGIN      
        1 tgtaattaac tgtattaccg gaaagtaccg acgaatttgg aatggttact tcttcgtttt
       61 tgggagttct taaccgcgta accaacaacg ttttctcaat cacatctccg gtaacatctc
      121 caatttttat gcggtcgcca actttaaaag gcctcatgta agttataacc aatccggcaa
      181 ccacatttgc aatggatgag tgtgaaccta acgaaaacaa aacaccgata aacaccgata
      241 ctcctttaaa tatgtttgag tcggaagcag gcagaaaagg gaaaatgagc acgaacatga
      301 atgcatacag caggaatttg acaatactga aggtaggcat tgcccaatcg gcatgaaatc
      361 ctgacacttt tagtttttct gagtctattt cagtaaaaat atatttcacg aacttaatga
      421 aatacttcat tacaaaataa atagccagaa tgctaaacag atttggcaaa taattccaga
      481 taccaataat tattgacttt aatggggaca aaatatattg aataagtttg tcggcccaac
      541 cccgtgtaat agggaaaata ctgaatattg cggataagga gatataaatc aggacaatat
      601 aaacaaacca gcgaaggagt ttggtaataa acaaaatgat ttggtatttc tgttcctcgt
      661 tcagaaaagt ataatccttg taagccagtt ttttgagcca cttctcttta ttgctgttga
      721 tggagttcat caacctgcgg tttccgagcc taattagcca tattaaaagc catgttaata
      781 aaattacaag taaaacaaga cctgttctga ttaatgttct ggataaactg ttttcgtatt
      841 ttgctttttt aattgaatcg gcaatcttaa ttttatactc atttcccagt tccgataaat
      901 ttttatcgtg ccacaaagca tctgtttcat tcaaacttaa aacgatggtc tttccgtact
      961 ggattgtata attatcagcg gtttcgaata cttttaacga atcagtgtta aaataatcat
     1021 ctttatataa ttcatgtatt ttttttgtaa gtatttctgc cctgtctttt gccgacaatt
     1081 cacctctttt tgaataaata tgaaaaaggg tatcgcccaa aactccaaca acaggataac
     1141 cttttgtgct ttttttaaga aattcaatct gctgtttctt ttcttccaaa tttctgtttt
     1201 ccttgtcttc tatttcctta agcttagtct gtaattcctc aatctgcgtt ttatcggttg
     1261 attttaaact gataagttgc gctttaagct cctccttctg aattgaatca tttatccgtt
     1321 gttcctcaac gttccgtaac aaagccgaaa tgtcgggtat caaatttgtt gaagaatcag
     1381 ttactttatt gttgttggtt tgttgagcat tgattgtgaa attggcaaga accaacacaa
     1441 tcagtaatat attcaatcta attttcttca tattttacat gtatgttatt cttataatca
     1501 acagtatacg attcttaaac ccggctcagg attgattttt gaaatatagg caaccttaat
     1561 ttctgttact atattgaact cgacaggtaa ttcacgataa attacttttc attttcactt
     1621 tccttcacct tttttgattc aaaatagaga tgaaggatta aattcaatgc atataggttt
     1681 tcggtatcat aatgtttgtc gtattgataa gttaggttac cctctaaggc gaagcggggc
     1741 ctccaggtaa cagtagtgcc tccaataaga cgtatcctgc tgaaagcaga atagcgggaa
     1801 tcccagtaag gttcagcatt tacataaggg acgaacgatg cattccctct tttaaactcc
     1861 ttttcaatca ttacccggta gcgaatccga taagagaatt cattattttc accaagccat
     1921 cgcaattcgg gtcgtaaacg atgcgaaagc aaaaaattgt taagcaaggg catcctcctg
     1981 tgaatttctc ctaagaccag attctcctgg taatctccat tttcacccag gctccatccc
     2041 cgcattacgc caccccttgc cagccagcct ttcatttgct gttcccgatt ttgatccatc
     2101 agcctggaga gatagatcct tttggtatgt ccccaggcat agtcagcctg caaatagtag
     2161 tttatatcgc gatacttgct ctcgtgatat tttgtaatgg gaataaaagc tgacagtcgc
     2221 cacgaaggat ttattctgta ccaaatatca gtttcgggcc agaactcaaa cgatgagttt
     2281 tgtgcactta cccagctagt attcagtata taaataaaaa ttcctatgcc tactgatcgt
     2341 aagaatagac ttcgtgtgag tttgaattta gccataccaa caaaagtcgt ttgaatagaa
     2401 ggtcttgtga atgtaaataa atattcaata cggtaagtgc tgaaagtcct ggtagattat
     2461 ttaatttgtc ctttgtcaaa gagatatttc gatccgaaaa aatagtacta
//
LOCUS       DASWBY010000240         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_384214, whole genome shotgun sequence.
ACCESSION   DASWBY010000240 DASWBY010000000
VERSION     DASWBY010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384214"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VF870_04375"
     CDS             <1..344
                     /locus_tag="VF870_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251451.1"
                     /translation="DAEIKNDDIDLTQESVKQLSTIERLRLEALGLESKKKKSDEIIV
                     PEKIKRDVEKKVNQTFKNGDVDFEAIDKMNVHKLRHYARAFPDFPIKGREISRANRDE
                     LVEWFRKMKND"
     gene            353..1267
                     /locus_tag="VF870_04380"
     CDS             353..1267
                     /locus_tag="VF870_04380"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX9251452.1"
                     /translation="MKNKISIIILSVLFSILIWGTVTLSDQFYSTKDFDVKVINQPTG
                     YACGVTNPEKISVKIKARGWQLLTLGLGPQTEYLVSADKDSGLITVDPFNEVDENTWL
                     SAGLSVAEISPREISFKVERLKYKRLKIEAETDLSFSDEYGLATPIRVYPDSVLVEGP
                     SSVLDKISEIKTKLVKLSALDNKIKLITDIEEPEGFKLEQNKAELTIDVQRIVEKTFE
                     NVKVIIKDIPKDRDVVLIPNTINCSLRGGINILGKINPNEITASLDYREIVYDTLGSV
                     QPNVVIPKNTQLVFIKPVRLNYIIKKFE"
     gene            1311..>1891
                     /gene="tmk"
                     /locus_tag="VF870_04385"
     CDS             1311..>1891
                     /gene="tmk"
                     /locus_tag="VF870_04385"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX9251453.1"
                     /translation="MFITFEGIDFSGKSTQVELLRDYLLEQKKNVHLIREPGGTEISE
                     KIREILLDKKNNAMLMETEIFLFSASRAQLVREVIRPYLKKGYYVISDRFHDSSTAYQ
                     GFGRGLSVDAILNIHNLAIGKTIPDITFFIDIPNNIALARKKNIAHSDLDRIEVSDDT
                     FYERVREGYLYLAKKEKRFIVIDGTRNIKQIHE"
BASE COUNT          696 a          253 c          362 g          580 t
ORIGIN      
        1 aggatgctga aattaaaaat gatgatatcg atttaactca ggaatcagtt aagcaattaa
       61 gcacgattga aagattaaga ttagaagcac ttggtttaga aagcaaaaaa aagaaatccg
      121 atgaaatcat tgtaccggaa aaaataaaaa gagatgtaga aaaaaaagtg aaccagactt
      181 ttaaaaacgg tgatgttgat tttgaagcta tcgataagat gaatgtacac aagttgcgtc
      241 attatgcaag agcatttcca gatttcccaa ttaagggaag agaaatttca cgggctaatc
      301 gagatgagtt agttgaatgg ttccgaaaaa tgaagaatga ttaattttag aaatgaaaaa
      361 taaaatttcc ataatcatat tatcagtatt gttttcgata cttatctggg gtacggtaac
      421 tttatctgat cagttttatt caacaaagga ttttgatgta aaagttatca accagcctac
      481 agggtatgca tgcggagtaa ctaatccaga aaaaatatca gtaaaaataa aagcaagggg
      541 ttggcagctg ctaacacttg gtcttggacc acaaactgaa tatttagttt ctgctgataa
      601 agatagcgga ctaattacag tggatccttt taatgaggtt gatgaaaata cctggttaag
      661 tgccggttta tcagttgctg aaataagccc aagagagatt tcatttaagg tagaaagatt
      721 aaaatacaag cgccttaaaa ttgaagcaga aacagatctt tcttttagcg atgagtatgg
      781 attagctaca ccgattagag tttatcctga ttcggtgctt gtagaagggc cttcaagtgt
      841 acttgataaa atttctgaaa tcaaaactaa acttgttaaa ctctcagcgt tagataataa
      901 aattaaactc atcactgata ttgaagaacc ggaaggtttt aaattggaac aaaataaggc
      961 tgaattaaca attgatgttc aaagaattgt tgaaaaaact tttgagaatg taaaagttat
     1021 tattaaagat attcctaaag atcgtgatgt agtacttatt ccgaatacta ttaattgcag
     1081 tttaagaggt ggaattaata ttcttggaaa aatcaatcct aatgaaatca cagcatcgtt
     1141 ggattatagg gagattgtct atgatactct cggcagtgtg cagccgaatg ttgttatacc
     1201 taaaaatact cagttagtat ttataaaacc tgttcggcta aattatatta taaaaaaatt
     1261 tgaataattt tagtcgcgat ataatcttaa aataaaggca aatcattttt atgtttataa
     1321 cttttgaagg aatagatttt agtggtaaat ccacacaagt tgaattattg agggattatc
     1381 ttttagaaca gaaaaaaaat gttcatttga tacgggagcc gggtggcacc gaaatctctg
     1441 aaaagataag agaaattctt cttgataaaa aaaacaatgc aatgcttatg gaaacagaga
     1501 tatttttgtt ttcagcaagc agagcccagt tggtaagaga ggttattcgt ccctacctta
     1561 aaaaaggata ctatgtaatt tctgatagat ttcatgattc ttctactgca taccagggat
     1621 tcgggagagg tttgtctgtt gacgctattc taaatattca taatcttgct ataggtaaaa
     1681 ctattccgga tataactttt tttattgata ttcccaataa tatcgcgctt gcacggaaga
     1741 aaaatatcgc tcattccgat ttagatagaa ttgaggtttc ggatgatact ttttatgagc
     1801 gggttaggga aggctatctt tatttagcaa aaaaagaaaa acgttttata gttattgacg
     1861 gcacacgaaa cattaaacaa atacacgaac a
//
LOCUS       DASWBY010000241         9354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_10670, whole genome shotgun sequence.
ACCESSION   DASWBY010000241 DASWBY010000000
VERSION     DASWBY010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10670"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2209
                     /locus_tag="VF870_04390"
     CDS             <1..2209
                     /locus_tag="VF870_04390"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX9251454.1"
                     /translation="FISRIDTAVDKELDKINADQLKGKIAIANGKKSYVEFEKIFQSN
                     RWKSLEKKGARVQRLLWASTGVKNPAYPDTIYVDELIGNLTVNTMPPATFNDFMDRGS
                     LGVTLDKNIEEANNQLNELKNIGIDLDKITNQLQVEGVQSFSKSFENLMKTIEEKIER
                     IKKEQKSFILYPGNYESEINNAVIELKKERIVERIWKKDFTVWSDSPNEITNRLGWLK
                     SPDVSLEMSDEIIEFVESVRKDGYTNALLLGMGGSSLAPEVFSLSFGVKEGYLDLSVL
                     DSTHPEAVLAYSKKLDPAKTLYIVSTKSGGTVETFSFMKFFYNQTLAKVGKENVGMHF
                     VAITDPGSGLETAAKQLNYRKIFLNDPNIGGRYAALSLFGIVPAALLGIDIKKLLNNA
                     QVMVCNSEGCNSPVRGDNTPAKLGVLMGELANRGRDKVTFITSNQLSYFGNWVEQLIA
                     ESTGKIGKGILPVVGEEVLSPDNYSNDRFFIYLRFEDDHKYDTKVKELIKAGHPAVEI
                     VLKDIYDLGGEYFRWEIATSIASWRTGITPFDQPNVEAAKILAREMLAEYSKEGKLTQ
                     PNPNFEKSGIKIYSNDDSKDFNSLVNEQLGKDDNDFRRKGNQYVSIQAYVKPDETVDK
                     LLHSFRTFLQKKYKSAITVGYGPRFLHSTGQLHKGDAGKGVFIQFISSSGNDAAIPDN
                     AGEEKSTMSFGTLITAQAFGDRKALINAERKVISFLFDDKNLINSIKLITDNF"
     gene            2281..2997
                     /locus_tag="VF870_04395"
     CDS             2281..2997
                     /locus_tag="VF870_04395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX9251455.1"
                     /translation="MEKTIRFEGIGDVLFRHSSRARYLNISVRPFHGVRVSIPTGMSY
                     NSATRLVNEKRLWIQQHLVKMKEFEKQQTMFDENSGYCTKHHKLYLSKANRKNISVRI
                     SRAKINVIYPTRINSDSIAVQAAIRKGIERALKVEAKQFLPDKVKHLAVKFEFNYNKL
                     TLKNIKSRWGSCSKKNNINLSVHLMRLPDHLIDYVILHELVHTVHHNHSKRFWAMLDT
                     VSGGAKILDKELGKHRIAIY"
     gene            3139..6234
                     /locus_tag="VF870_04400"
     CDS             3139..6234
                     /locus_tag="VF870_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEX9251456.1"
                     /translation="MNKKLYFKILLILLIILPPFIKSQTNNQEFRATWVITWEYISSG
                     STVEVNKARIREILDNHKKANMTSVLFQIRQSGTAYYQSSYEPWGYYAGYTYPGFDPL
                     QYAIEEAHKRGLELHAWFNTFSVSSTQPGTIPVEHPEWICRDENGNPMTSYMAASPGM
                     QAVRDYTINVAMEIVRNYDIDGLHLDYIRWNEYDTDDMLRPLSQIEQESRQDGFFTDQ
                     TINKLTSPQSTNRFLYDVEHPFSAGVPAGFNTWEDWWRWSVTEFVRTLHDSIQTVKPW
                     VRLSPAALGKYKAGGASGWNGYYVVYQDAALWFNEGYVEQLTPMHYHWLTGEEMFNAI
                     STDWEPNIQPGIQAGRLYSCGPASYRLDENNVWSNHVGIVNRMRDKTWVDGFQFFSYG
                     SWKGYDYWDEAGSTFFDNKVKVRDINIVSLPAAPTISLNQIDSLNYEIFVNPDQATID
                     NQWFAVYRSEDDVLDLNNDVIVDLHFGNSNYSIINTYTGLQNFNGTYKYFATTLNRYW
                     NESEISNQVSTEQIPSFAPIVVSTTPAENGVLNIPNNIVIEFSKTMDVASFTNALTIT
                     PSVQITNLTWNESNKVLSIVTEGHQHQTAYILTIDSSVTDINGKMLDGNGNGIEGDPF
                     VLHYQTNEEDILGPQILYSYPTDNDTSVDVGSILTIVFDEEIDAQSLSSSDIIVTNEG
                     LPVSFSFQHTKSLDGKSIICIQPLSLFYRSANFSVTISGDISDTLGNISGTESQINFT
                     TSELSYSEIKMIDNFTLPGDWKQPDYSGSTKGILTSGTHFEFTNLVYLPATNPKKSAK
                     LSYLWDENATDFLIREYLAGGTPQSIYFDTSYVLQSYVYGDGSNTRFRFCIDEYTGSA
                     WGDHEVSKWVTIDWYGWKLVEWKLNDPNSVGIWIGDGNLTGQYFRIDSYQLTKTTESS
                     FSGVIYFDDLRAVKKINAPTEVNDKHNLIPKQYLLSQNYPNPFNPETIINWQVPKGGH
                     TTLKIYDVLGNEVAILVDEFQPAGKYEIKFNASSISSGVYFYRLQSDLFIETRKMIYQ
                     K"
     gene            6294..8942
                     /locus_tag="VF870_04405"
     CDS             6294..8942
                     /locus_tag="VF870_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251457.1"
                     /translation="MSQGKVYLVLASDTAIWDAMDVSKYHCTYNLDLFTAVNSNTSVV
                     MSESFRNQITDSYGGKLKMTWYMMAGNIFRYATNNNVPINNTMTLYLMKKYFGDKVEL
                     WNDELSLHYHTFFWSDYDGDGKYWWNQSETFMESKDDFDFTLAQFLLDENVFPVSFRS
                     GWYYMDNDWQNYLNEILPYSLHNEYPNINHDFTEPIDNNFDWSQSSPEFVPFHPSSEN
                     YQLPGNDKGWNVRAKYMGSTTQQLMNEIFSKADQGTDQLVCLWSHLPDANFMNEIQNV
                     NNLAHQAELNYPNVQFKYCTAVEAYQLWLQSNDLVKPVLQLSEEINGSDYKYLISTDE
                     EIFQAQPFVAIKDRYERYFKAECELIEQNLWRTVSSYSMSEIGKVGAAVTDTVGNLST
                     SFIKYLPDDLFIDNSDPQYQELYGNWNTSTTSAWDVNSRIVNLNNGDSAKVRWNLDVG
                     QSGLYNLFIQFPEIVNPVDTIKVELFQNDQIKDTIFFTSTDKFYKWGFVATLNLDNSQ
                     NNFLEMSARNFSQDSRSLAMDVIKLTAYIRDKQLVADQEFVDAGETSIDDTLQFTIEL
                     SNIGIDELTVSDIFSTNGNIEPITTLPMIITGMDKIDLQLVFIPQQLGIVEDTIVIIS
                     DDPINSIKKIPFIVNVENYFVIIDNDDIENYSETGTWATSVAQAYGPSSRYAYIQYTP
                     NGPTAAFTFQLNRSGYYDIFEIIPQTINSANNALYKILVGDRVIDSLYLNQNEGSGNW
                     KNIGRFYLPADSSVSIKVIDSGESSSGPVIRADAFKVLLYEESPINVDEDNSIQVNEY
                     QLDQNYPNPFNPSTRISWQSPVGSHQTLKVFDVLGNEISTLVDEYEPAGRYELEFNAA
                     ILPSGVYFYRLQSGSYVETRKMIYLK"
     gene            9064..>9354
                     /locus_tag="VF870_04410"
     CDS             9064..>9354
                     /locus_tag="VF870_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251458.1"
                     /translation="MNIKRLTKSTIVLLFLTSFNLFSQNYPDQFYVLGIDSIYQNIDS
                     NVGMMLSNDGKSMMLQDGVNDGYIILKPQYSQSPFNEGLPSWNGSAADANNGF"
BASE COUNT         3232 a         1429 c         1804 g         2889 t
ORIGIN      
        1 ttttataagc agaatagata cagcagttga taaagaatta gataaaatta atgctgatca
       61 gcttaagggt aaaattgcaa ttgccaacgg aaagaaatct tatgtggaat ttgaaaaaat
      121 atttcaatca aatagatgga agtctttgga aaaaaaaggc gctagggttc aaagattact
      181 ttgggccagc acgggggtta aaaatccggc ttatccggat acaatttatg ttgatgagtt
      241 aattggcaat cttactgtta acactatgcc acccgctact tttaatgatt ttatggatcg
      301 cggaagttta ggagtcactc ttgataaaaa tattgaagaa gcaaataatc agttgaatga
      361 attaaaaaat atcggaatcg acctcgataa aattacaaat caattgcagg tagaaggagt
      421 gcagtcattt tcaaaatcat ttgaaaatct gatgaagaca attgaagaaa aaatagaacg
      481 gattaaaaaa gaacaaaaat catttattct gtatcctggt aattatgaat cagaaattaa
      541 taatgccgta atagagttaa aaaaggagcg gattgtagaa agaatctgga aaaaagattt
      601 tacagtttgg agcgacagcc cgaatgaaat tacaaacaga cttggctggt taaaaagtcc
      661 tgatgtttca ttagaaatgt cggatgaaat tattgagttt gtagaatcag ttagaaaaga
      721 tggatataca aatgcacttt tacttggaat ggggggctca agtcttgccc ctgaagtttt
      781 tagtttatcg tttggagtaa aggaaggtta tctggattta tctgttttag acagtacaca
      841 tcccgaagca gttttagctt actcaaaaaa acttgatccg gctaaaaccc tctatatagt
      901 ttccacaaaa tccggcggca cagttgaaac attttcattt atgaaattct tttataatca
      961 aacccttgca aaagtaggta aagaaaatgt tggaatgcat tttgttgcaa tcactgaccc
     1021 tggcagcgga ttggaaactg cggctaaaca attgaactac cgcaaaatat ttttaaatga
     1081 tccaaacatc ggcggaagat atgcagcgct atcgttattt ggtattgtac ctgctgcatt
     1141 acttggaatt gatataaaga aattacttaa caatgctcag gtaatggttt gcaacagtga
     1201 aggatgtaac tctccagttc gcggtgataa tactccggct aagcttggag ttttgatggg
     1261 agaacttgca aatcgcggta gagataaggt cacttttata acttcaaatc agctatcata
     1321 ctttggtaat tgggtggaac aactaattgc tgagagcacc ggaaaaatcg gaaaaggaat
     1381 tcttccagtt gttggtgaag aagttttatc tcccgataat tattcgaatg acagattttt
     1441 tatatacctg cgattcgaag atgatcataa atacgataca aaggttaaag agttaattaa
     1501 ggctggtcat cctgcggtag aaatcgtgtt gaaagatatt tatgatttag gcggcgaata
     1561 ctttagatgg gagattgcaa cttctattgc gagctggaga acaggaataa ctcctttcga
     1621 tcaaccgaat gttgaagcag caaaaattct cgcgagagaa atgcttgctg aatattcgaa
     1681 agaaggaaaa cttacccagc caaatccaaa tttcgaaaaa agtggaatta aaatttattc
     1741 taatgatgat tcaaaagatt tcaattctct tgtcaacgaa cagcttggta aggatgataa
     1801 tgattttcgc agaaagggta atcaatatgt ttccatccag gcttatgtaa agccggatga
     1861 aacagttgat aaacttcttc actcttttag aacttttctt caaaagaaat ataagtcggc
     1921 aattactgtt ggatacggac caagatttct tcattcaacc ggacagttgc acaaaggcga
     1981 tgctggtaaa ggtgtattta ttcagtttat ttcatcaagt ggaaatgatg ctgcgattcc
     2041 ggataatgcc ggtgaggaaa aatcaacaat gtctttcggt acattgataa cagcgcaggc
     2101 atttggagat agaaaagcat taatcaatgc agaacgaaaa gtgatttcat ttttatttga
     2161 cgataaaaac ttaataaact caattaaact aatcacagac aatttttaaa ttaaaatatt
     2221 ctttatgatg aaaatatatt tcacgctaat ttatttaaat aatctttcga aggaatgata
     2281 atggaaaaga caattcgctt cgaaggaata ggtgatgtat tattcagaca tagcagcaga
     2341 gcacgttatc ttaacatttc cgtgagacca ttccatggtg taagagtatc aatacctact
     2401 ggaatgagtt ataacagtgc gacccgtttg gtaaatgaga agaggttatg gatacaacag
     2461 catcttgtaa agatgaaaga atttgaaaaa cagcagacta tgtttgatga gaattccggt
     2521 tattgtacaa aacatcataa actatatttg agcaaagcaa acaggaaaaa tatttctgta
     2581 agaatttcaa gggcaaagat taatgtaatt tatccaacca gaattaattc tgattcaatt
     2641 gcagttcaag cagcaatcag aaagggaatt gagcgagcgt taaaggtaga ggcaaaacaa
     2701 ttccttccgg ataaagtaaa acatttagct gtaaaatttg agtttaatta taacaagctc
     2761 accttaaaaa atattaaatc aagatgggga agctgttcaa agaaaaataa tattaattta
     2821 agcgtccatc ttatgcgatt gcctgaccat ttgattgatt atgtaattct tcacgagttg
     2881 gtccatacag ttcatcacaa tcacagcaag cgattttggg caatgctgga tacggtttcc
     2941 ggcggtgcaa agattttaga taaagagtta gggaaacacc gcatagcaat ttattagaac
     3001 ccagcaaacc ttgtgagtcc taaatcatat caatcttcaa gcatcaacat tatgattaaa
     3061 atcttttatg agcaatttta tttgcataaa tttataacta ataaatgaga tttattaact
     3121 ctttacagat tgtgtatgat gaataaaaaa ttatacttca aaatattact tatacttctt
     3181 ataatacttc ctccttttat aaaatcccaa acaaacaatc aagaattcag ggcaacctgg
     3241 gttataacgt gggaatatat tagttccggc agtaccgtgg aagtaaataa agcacgtatc
     3301 agagaaattt tggataatca taaaaaagca aatatgactt ccgtgctttt tcaaatcaga
     3361 caaagcggaa cggcatatta tcaatcttct tatgagccgt ggggatatta tgccggatat
     3421 acttatcctg gatttgaccc acttcaatat gcaatagaag aagcccataa gaggggtctg
     3481 gaattacatg cttggtttaa tacattcagc gtttcatcca cacagccggg cactattccg
     3541 gttgaacatc cggaatggat ctgcagagac gagaatggaa atcctatgac ttcatacatg
     3601 gctgcatctc cgggaatgca ggccgtaaga gattatacaa ttaatgtagc gatggaaatt
     3661 gtaagaaatt atgatattga tggcttgcat ctggattaca ttagatggaa tgagtatgat
     3721 actgatgaca tgctgcgtcc gctttcacaa attgaacagg aatccaggca ggatggattt
     3781 tttacggatc aaactattaa taagttaaca tctccgcaaa gtacaaacag gtttttgtat
     3841 gatgttgaac atcctttcag tgccggagta cctgctggat ttaatacctg ggaagattgg
     3901 tggagatggt cggttacaga atttgtcagg actttacacg attccataca aaccgttaaa
     3961 ccctgggttc ggttatctcc ggcagcttta ggaaaataca aagccggcgg tgcttcaggt
     4021 tggaatggtt actatgttgt ctaccaggac gctgcgttat ggtttaatga ggggtacgtt
     4081 gaacaattaa cgccaatgca ctaccattgg ttaacaggtg aagaaatgtt taacgcaata
     4141 agcacagact gggaaccaaa tattcagcct ggaattcaag ctggaagact ttatagctgc
     4201 ggaccggcat cttacaggct tgatgaaaat aatgtttggt caaatcatgt cggaattgtt
     4261 aacagaatgc gggataaaac atgggttgat ggttttcaat ttttcagtta cggcagttgg
     4321 aagggttatg attattggga tgaagcagga tctacattct ttgataataa agttaaagta
     4381 agagatataa atatcgtatc cttaccagct gcaccaacaa tttcattaaa tcaaattgat
     4441 tctcttaatt atgaaatttt tgtaaatccg gatcaggcaa ctattgataa tcaatggttt
     4501 gctgtttaca gatctgaaga tgatgtgttg gatcttaata atgatgtgat agttgattta
     4561 cattttggaa attctaatta cagtattatt aatacctata ccggattaca gaattttaac
     4621 ggaacgtata aatactttgc aactacactt aacagatact ggaatgaatc tgaaatttca
     4681 aatcaagtgt caactgaaca aataccttca tttgcgccaa ttgtagtatc aacaacacct
     4741 gctgaaaatg gagtgttgaa tattccaaat aatattgtaa ttgaattttc aaaaactatg
     4801 gatgtagctt cttttacaaa tgcactaaca atcacaccat cagttcagat tactaatttg
     4861 acctggaacg aaagtaataa agtgctctca atagtaacag aaggtcatca gcatcaaact
     4921 gcttatatct taacgattga ttcaagtgtg actgatatta atggcaaaat gcttgatggc
     4981 aatggtaacg gaattgaagg cgatccgttt gtgcttcatt atcaaacaaa tgaagaagat
     5041 attcttggtc cacaaatctt atatagttat cctaccgata atgacactag cgtagatgtc
     5101 ggttctattc taacaattgt atttgatgaa gaaatcgatg cgcaatcgtt atcatcttct
     5161 gatataattg taacgaacga aggattacct gttagtttta gttttcaaca tacaaaatct
     5221 ttagatggga aaagtataat ttgcattcag ccgcttagtt tattttaccg aagtgcaaat
     5281 ttttctgtta caattagcgg agatatttct gatactttag gcaatatatc gggcacagaa
     5341 tcacaaatta attttactac atcagaatta tcatacagcg aaataaagat gattgataat
     5401 tttactctgc cgggtgattg gaaacaacct gattacagcg gctctacaaa aggcattcta
     5461 acctcaggga cgcattttga atttacgaat cttgtttatc tgccggcaac caatccaaaa
     5521 aaatctgcaa agcttagtta tttatgggat gaaaatgcaa ctgatttttt aattcgtgaa
     5581 tatttagccg gaggaacacc tcaatcaatt tattttgata cgagttatgt tttacaaagc
     5641 tatgtctatg gagatggcag caatacacgg tttagatttt gcatcgatga atacactgga
     5701 tcagcctggg gtgatcacga agtttcgaaa tgggtaacaa ttgattggta cggatggaag
     5761 ttagttgaat ggaagcttaa tgatccaaac agcgttggta tctggatagg tgatggtaat
     5821 cttaccggac aatattttag aattgatagt tatcaattaa caaaaacaac agaaagttca
     5881 ttttccggag taatttattt tgatgatctt agagctgtta aaaaaattaa cgctcctaca
     5941 gaagtaaatg ataaacacaa cctaatacca aagcaatatt tactgtcaca aaactatccg
     6001 aatccattta atcccgaaac tattattaac tggcaagttc ctaaaggcgg acatacgaca
     6061 ctaaaaattt atgatgtact tggtaatgag gttgcaattt tggttgatga atttcaacct
     6121 gcaggaaaat atgaaataaa atttaatgcc tcttcaatat ccagtggagt ttatttttac
     6181 agattgcagt ctgatctgtt cattgaaaca agaaagatga tttatcaaaa atgaaaattt
     6241 ataataaatt cagtttaata ttgttaaccg tccttttatt tgttgagaca gctatgtcgc
     6301 aaggtaaagt ttatttagta cttgcttctg atactgcaat atgggatgct atggatgtat
     6361 ccaaatatca ttgcacttac aatctggacc tttttactgc ggttaacagc aatacaagtg
     6421 ttgtaatgag tgaatcattc aggaaccaga taactgattc ttacggcggc aaacttaaaa
     6481 tgacctggta tatgatggcc ggaaatatct ttcgttacgc cactaataat aatgttccga
     6541 tcaataacac tatgactttg tatttgatga aaaaatattt tggcgataaa gtagaattgt
     6601 ggaatgatga actgtctttg cattatcata catttttctg gtcagattat gatggtgatg
     6661 gtaaatactg gtggaatcag tccgagactt ttatggaaag caaagatgat tttgatttta
     6721 cacttgcaca atttcttttg gatgaaaacg tttttcctgt ttcttttaga agcggctggt
     6781 attatatgga taacgactgg caaaattatc tgaatgaaat tctgccttat agtcttcata
     6841 atgaatatcc aaatataaat catgatttca ctgagccaat agataataac tttgattggt
     6901 cacagtcatc tcccgaattc gttccttttc atccatcttc agaaaattat cagttgcccg
     6961 gcaatgataa agggtggaac gtaagagcaa aatatatggg ttcaacaact cagcaattga
     7021 tgaatgaaat attttcaaaa gctgatcagg gtacagacca gcttgtatgt ctttggtccc
     7081 atttgccgga tgcgaatttt atgaatgaaa ttcaaaatgt taataacctg gcacatcagg
     7141 cagaattaaa ttatccaaat gttcagttca agtactgcac tgctgttgaa gcatatcagt
     7201 tatggctgca gagcaatgat ttagtaaaac cagttttgca attatccgaa gaaataaatg
     7261 gttcggatta taagtattta atcagcactg atgaagaaat atttcaggca caaccatttg
     7321 tagctataaa ggatagatat gaaagatatt ttaaagctga atgtgaatta attgagcaaa
     7381 atttatggag aacagtttca tcttattcga tgagtgaaat cggaaaagtt ggtgcagcag
     7441 ttacagatac tgttggtaac ttatccacat catttattaa atatctgccg gatgatttgt
     7501 tcatagataa tagcgatccg caatatcagg aattgtatgg aaactggaac acctcaacaa
     7561 catctgcctg ggacgttaat tcaagaattg taaacctgaa caatggtgat tcggctaaag
     7621 taagatggaa tttggatgtc gggcaatccg gcttatacaa tctctttata caatttcctg
     7681 agatagttaa tccagtggac acaattaaag tagagttatt tcaaaatgat caaattaaag
     7741 acaccatatt tttcacatcg actgataaat tttataagtg gggttttgta gcaacattga
     7801 atttagataa cagccaaaat aattttcttg aaatgtcggc tagaaatttt agccaggata
     7861 gcaggtctct ggctatggat gttattaagt taacagcata cattagggat aaacaattgg
     7921 tagcagatca ggagtttgtt gacgcaggag aaaccagtat tgatgatact cttcagttta
     7981 ctattgaatt gagtaacatt ggaatagatg agttgacggt tagtgatatt ttttcgacaa
     8041 atggaaatat tgaaccaatc actactttac caatgattat tacaggaatg gacaaaattg
     8101 atttacaact tgttttcatt cctcaacaac taggaattgt agaagataca atagtaataa
     8161 ttagtgatga ccccataaat tcaatcaaaa aaattccatt cattgttaat gtagaaaatt
     8221 actttgtgat tattgacaat gatgatatag aaaattatag tgaaacagga acatgggcaa
     8281 ccagtgtagc ccaggcttat ggaccatcaa gtagatatgc gtatatacaa tacacaccca
     8341 atggtcctac tgctgcattt acgtttcagc taaacaggag tgggtattat gatatttttg
     8401 agatcattcc acaaactata aatagtgcta ataatgcttt gtacaaaatt cttgtaggag
     8461 atagagttat tgattcattg tatttaaacc aaaacgaggg aagcggaaac tggaagaata
     8521 taggtagatt ttatttacct gcggatagtt ctgtttcaat aaaagtaata gatagtggcg
     8581 aaagctcttc gggtcctgtt ataagagcag atgcatttaa agttttgtta tatgaagaaa
     8641 gtcctatcaa tgttgatgaa gataattcta ttcaggtaaa tgagtatcag ttagaccaga
     8701 attatcctaa tccatttaat ccaagtacaa gaatcagttg gcaatcgcca gtgggcagtc
     8761 atcaaacatt aaaagtattt gatgtgttgg gaaatgagat ttcaacactg gttgatgaat
     8821 atgaaccagc aggaaggtat gagcttgagt ttaatgctgc tatactgcca agcggagttt
     8881 atttttacag attgcagtct ggttcgtatg ttgagacaag aaagatgatt tatttaaagt
     8941 agtattctga tcgctaaata agtttaacta aaaatatttc ggtaataatc tttaaagaac
     9001 cataatgttt ttataacttg atatgaatta ataaattaat acaaaacaca accaaattta
     9061 attatgaata taaaaagact gactaaaagc acaatagtat tgttatttct tacttctttt
     9121 aatttgttct cacaaaatta tccggatcaa ttttacgttt taggtattga ttcaatttat
     9181 caaaacattg actcgaatgt aggaatgatg ctaagcaatg atggtaaaag tatgatgctt
     9241 caagatggag ttaatgacgg ttatataatt ttaaaaccac aatactctca atcacctttt
     9301 aatgaaggtt taccatcgtg gaatgggagt gcggctgacg ctaacaatgg tttt
//
LOCUS       DASWBY010000242         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_722982, whole genome shotgun sequence.
ACCESSION   DASWBY010000242 DASWBY010000000
VERSION     DASWBY010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722982"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VF870_04415"
     CDS             <1..330
                     /locus_tag="VF870_04415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9251459.1"
                     /translation="GIDIINNVGTPVKATGKGKVIFAGIKGGYGLTVEIDHGFGYQTV
                     YGHLSKVTVKQGQIVNRNQIIAKSGNSGLSSGPHLHYEVLHNGQYLNPSEFFFDEYSY
                     FESNHSN"
     gene            353..574
                     /locus_tag="VF870_04420"
     CDS             353..574
                     /locus_tag="VF870_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX9251460.1"
                     /translation="MIWTVELASYLDDAPWPATKEELIEYAERIGAPYEVMENLKELE
                     DSDEPYESIEDIWPDYPSDEDFFYNDEDE"
     gene            602..1720
                     /locus_tag="VF870_04425"
     CDS             602..1720
                     /locus_tag="VF870_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251461.1"
                     /translation="MSIFLDEIAGNDKVKIILSNILQSGNIPHAFLFTGADGVGKENA
                     AICFTKALNQNIKSNEKSKSISALIDNLTEPYIKYILPLPRGKSETDQNDPYEKLNED
                     EIDLINSEFKKKSQNHFYKIQIPRANFVKISSIRDIKKFLSFNYEDIKYRVILISQAH
                     LMNEEAQNALLKNLEEPPEGVVFILCTPSPEKLRETIRSRCWNVHFQPLTQNDLSNIL
                     IEKFEMEYPIVKEVVPFSGGSVQTAISLIENNFEDLLDKTIKILRYSFGKKYHSALME
                     FEEMLSESDTLRIKLVITMIIIWLNDFQKYRLNEQNSVFFSKQIETLDKFKAKFPDVD
                     VADVANNLDRISSSFKNNINLNIAVSNIVFQLSKLTSK"
     gene            1798..2976
                     /locus_tag="VF870_04430"
     CDS             1798..2976
                     /locus_tag="VF870_04430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9251462.1"
                     /translation="MKKVVIVSAKRTSIGSFNGALSSFSSGQLGSFAIKAVLEDSKID
                     TALIDEVIMGNVLMGGQGQAPARQAAIFAGLPNKTECMTINKMCGSGLKSVMLAQQAI
                     ALGDAEIIIAGGQESMSNAPYLLPNARTGYRLGHGEIKDSIVLDGLWDVYNNMHMGNC
                     AESCARDFSFTREELDSFTIQSYKRAIEANNSGRFNDEITPVKITSRTGELIVDKDEE
                     PGKVKFDKIPTLKPVFDKNGVITAANASSINDGAAALLLMSEEKAKELGLKPLVEIIA
                     QSSAAKAPIEFTTAPADAINKVLKKAGMKTTDIGLYEINEAFAVVSLAVNKLLGLRSE
                     NVNVNGGAVALGHPIGASGARILVTLIHEMKKQKTEFGLASLCIGGGEASALIVKNYN
                     "
     gene            2991..>3082
                     /locus_tag="VF870_04435"
     CDS             2991..>3082
                     /locus_tag="VF870_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9251463.1"
                     /translation="MDIKKIAVIGGGTMGNGIAHVFAMKGFDVFL"
BASE COUNT         1089 a          471 c          588 g          934 t
ORIGIN      
        1 ggaattgata ttattaataa tgtaggaact cctgttaaag cgactggcaa gggtaaagta
       61 atctttgctg gtattaaggg tggatatggt cttacagttg aaattgatca tggatttggt
      121 tatcaaactg tatacggtca tttatccaaa gtgactgtaa aacaaggaca gattgtaaat
      181 cgaaaccaaa taatagcaaa atccggaaac tcaggtttat cctctgggcc acatttgcat
      241 tatgaagttt tacataatgg gcaatactta aatccatcag aatttttctt tgatgaatac
      301 agttatttcg aatctaatca tagcaattaa aattttggag gatttcgtat aaatgatttg
      361 gacagtggaa ctcgcctctt atttagatga tgctccttgg cctgctacta aagaagagtt
      421 aatagaatat gccgaacgta tcggcgcacc ttatgaagtt atggaaaacc ttaaggaact
      481 tgaggattct gatgaaccgt atgaaagcat tgaggatatt tggcctgatt atccctccga
      541 tgaagacttt ttctataatg atgaggatga ataagttttg gcgccttgag cgcctttttt
      601 tatgagtatt tttttagatg aaatagccgg gaatgataaa gtaaagatta ttttatccaa
      661 tattctgcaa agtggtaata tccctcatgc atttctgttt acaggcgcag atggggttgg
      721 gaaagagaat gcagcgattt gttttaccaa agcattaaat caaaatataa aaagcaatga
      781 aaaatcaaaa tcaatatctg cactaattga taatctcaca gaaccttata ttaaatatat
      841 tttaccgctt ccaagaggta aaagtgaaac cgaccagaat gatccctacg aaaaactaaa
      901 tgaagatgag attgatctca ttaattcaga gtttaaaaaa aaatcgcaaa accattttta
      961 taaaattcag attccgcgtg ctaactttgt taaaatcagc agtataaggg atatcaagaa
     1021 atttctttcc tttaattatg aagatattaa gtatcgagta atccttatct cgcaggcaca
     1081 tctgatgaat gaagaagcac aaaatgctct gttaaaaaat ttagaagaac caccagaagg
     1141 agttgttttc attctttgta cgccttcacc agaaaaatta cgtgagacaa ttagatcccg
     1201 atgctggaat gtccatttcc aaccacttac acagaatgat ctctcaaata tattaataga
     1261 aaaatttgaa atggaatatc ctatcgtaaa agaagtggta ccattttccg gcggatctgt
     1321 gcaaactgct atctctttaa ttgaaaataa ctttgaagat cttcttgata aaacaataaa
     1381 aattttacga tattcatttg gtaaaaaata tcattcagct ttaatggaat tcgaagagat
     1441 gttaagtgaa tctgatacac taagaattaa actagtaata acgatgataa tcatttggct
     1501 gaacgatttt caaaaatata gattaaatga gcagaatagt gttttctttt caaaacagat
     1561 agaaacgctt gataaattta aagccaagtt ccctgatgta gatgttgctg acgtagcaaa
     1621 caacttagat cgaatttcat cttcatttaa aaacaacatc aacctaaata ttgcagtcag
     1681 taatatcgtt tttcaattat ctaaactaac ttcaaaataa cataacttct ttctgattct
     1741 ttaatattat ttctttattt ttgttcaaaa ataaaatcat tttcattaag ggacattatg
     1801 aaaaaagtag taattgtatc agcaaaaaga acatccattg gatcattcaa cggggcatta
     1861 tcatcattta gttcaggaca actaggaagt ttcgctatta aagctgtttt agaagattct
     1921 aaaatagata ccgctctaat tgatgaagtt attatgggta atgttctcat gggtggccaa
     1981 gggcaagctc ctgcaagaca agctgcaatc tttgccggac ttccaaataa aactgaatgt
     2041 atgacaatca ataaaatgtg tggcagcgga cttaaatccg taatgcttgc tcagcaagct
     2101 attgcacttg gcgatgctga aataattatt gcaggagggc aagaaagtat gagcaatgca
     2161 ccttatcttt taccaaatgc aagaacaggc tatagacttg gtcacggaga aataaaagat
     2221 tcaattgttc tggatggact ttgggatgtt tataataata tgcatatggg aaattgtgct
     2281 gaatcctgtg ccagggattt ttcatttact cgagaagaat tagatagttt tacaatacaa
     2341 tcatacaaac gtgcaattga agcgaataac tccggtagat ttaatgatga gattacacct
     2401 gtcaaaatca cttcaagaac tggagaatta attgtagata aggatgagga gccagggaaa
     2461 gtcaagtttg ataaaattcc aactcttaaa ccagtatttg ataaaaatgg ggttataaca
     2521 gcagcaaatg catcaagtat aaatgatggt gctgccgcat tactcttaat gagcgaagaa
     2581 aaagcaaaag agctgggatt aaaacccctg gttgaaatta ttgctcaaag ttctgcagca
     2641 aaagcaccga ttgaatttac aacagcgccg gctgacgcta taaataaagt tttgaaaaag
     2701 gccggaatga aaaccacaga tattggttta tatgagatta acgaggcatt tgctgtcgtt
     2761 tcattggctg taaataaact gcttggttta agatctgaaa atgtaaatgt gaacggtgga
     2821 gcagttgctc ttggtcatcc aataggagcc agtggagcaa gaatattagt aacactgatc
     2881 cacgagatga aaaagcagaa aactgagttt ggtttagctt ctttatgcat cggtggtggt
     2941 gaggcatcag cattaatcgt aaagaattac aactaaaaga aagattcaaa atggatatca
     3001 aaaaaattgc agttattggt ggcggtacaa tgggaaacgg tattgctcat gtttttgcta
     3061 tgaaaggttt tgatgtattt ct
//
LOCUS       DASWBY010000243         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_195992, whole genome shotgun sequence.
ACCESSION   DASWBY010000243 DASWBY010000000
VERSION     DASWBY010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195992"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VF870_04440"
     CDS             <1..212
                     /locus_tag="VF870_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9251464.1"
                     /translation="KFPSGNHGYDPSNIDMHGIFYAIGPDFKSGYTCGTLNNIDIYPL
                     LAKILHIFPNNNIDGKLERIEFLLK"
     gene            315..1586
                     /locus_tag="VF870_04445"
     CDS             315..1586
                     /locus_tag="VF870_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9251465.1"
                     /translation="MNHLNLDCTKGKLKNGLEYILYEDKSLPLVSVNIWYKVGSAYET
                     KGKTGLAHLFEHMMFQGSENVPKEMHFRYIQEAGGNLNGSTSTDKTNYYEKLPSNSLE
                     LALWLESDRMGYFLPSLTQSKLDNQKDVVKNERLERYDNQPYGLAWELINTNLYPEGH
                     PYSWSTIGFLKDIESYTLDDVKNFFKKYYSPNNACLVVAGDFEKENAVQLIEKYFGSI
                     EPFESNHKPESNKLTLKENILIEHKDNVQLDRIYLAWPSDFIFGKDDSALEVAADILT
                     GSKNSRLYNKLVFDLQIAQDVTAFQYSGKFGGQFMIISTARPGTNLDELKRIILDEVN
                     TLANTAVSDKELEKSKNGIKAQFVFAMQNLDTVADYLNHYNYYLNEPNYFERDLERYT
                     DVNKISLKGAVQNYLTKPFVELRITPKETTK"
     gene            1583..>1829
                     /locus_tag="VF870_04450"
     CDS             1583..>1829
                     /locus_tag="VF870_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251466.1"
                     /translation="MIDRKNKPAVKSEINFKLPKANEFLLDNGLRVIFIQKDKLPLER
                     LNLIINAGSKYDPENKKGLSYFTSMLLDEGADGMDALQ"
BASE COUNT          675 a          288 c          303 g          563 t
ORIGIN      
        1 ataaatttcc atcaggcaat cacggatatg atccatctaa catcgatatg catggaatat
       61 tttatgccat tggtcctgat tttaaatcag gttatacctg cggtacatta aacaatattg
      121 atatttatcc acttcttgca aaaatacttc atatctttcc taacaataat attgatggaa
      181 aacttgaaag aattgaattt ttgttgaaat aaaactgaat tttcgtagcg cagacttaag
      241 tctgcgaaat taaactaaga atattaacac agactaaaga ctgtgttacg aaatatctaa
      301 agaggaaaca aaatttgaat catcttaatc ttgattgtac aaaaggcaaa ttaaaaaacg
      361 gactcgaata tattttatat gaagataaat ctttacctct tgtatcagta aacatttggt
      421 acaaggtagg ttcagcatat gaaacaaaag gcaaaacagg tttagcacac ctttttgaac
      481 atatgatgtt tcagggatca gaaaatgttc ctaaagaaat gcactttagg tacatacagg
      541 aagctggcgg aaatttgaat ggttctacct caacagataa aacaaattat tatgaaaaac
      601 ttccatccaa ttctcttgaa ctcgcactct ggcttgaatc cgacagaatg ggatattttc
      661 tgccctcact aactcaatcc aaacttgata atcaaaaaga tgtagtgaag aatgaaagat
      721 tggaaagata cgataatcag ccttatggtt tggcgtggga attaattaat acaaatcttt
      781 atcctgaagg tcatccatac agctggtcaa ctatcggttt tttaaaagac attgaaagct
      841 atacacttga tgacgtaaaa aactttttca aaaaatatta ttcgccgaat aatgcctgtc
      901 ttgttgtagc cggagatttt gagaaagaaa atgctgttca gcttattgaa aaatattttg
      961 gatccatcga accgtttgaa tctaatcaca aacctgaatc aaataaacta actttaaaag
     1021 aaaatattct gattgaacac aaagacaatg tacaacttga taggatctat cttgcatggc
     1081 cttcagattt tatttttggt aaagatgatt cagcattgga agttgcagca gatattttaa
     1141 ccggttcaaa aaattccaga ctttataaca aattagtttt tgatttacag attgcccagg
     1201 atgttactgc atttcaatat tcgggtaagt ttggcggaca atttatgatc atttcaactg
     1261 ctcgtccggg gactaatctt gatgaattaa aaagaataat actggatgaa gtaaatactt
     1321 tagcaaatac agctgttagc gataaagaat tagaaaaatc taaaaatgga attaaagctc
     1381 aatttgtatt tgctatgcaa aaccttgata ctgttgcaga ttatcttaat cactacaact
     1441 attatttaaa tgagccaaat tattttgagc gtgatcttga gcgttacact gatgtgaaca
     1501 aaatttcctt aaaaggcgca gttcaaaatt atcttactaa accattcgta gaacttcgca
     1561 tcacaccaaa ggaaacaaca aaatgatcga tagaaaaaat aaacctgctg taaaatcaga
     1621 aattaatttt aagcttccca aagcaaatga atttttattg gataacggtt taagggtaat
     1681 cttcattcaa aaagataaac tgcctttaga gagattgaat cttataataa acgcaggaag
     1741 caaatatgat cctgaaaaca aaaaaggatt gtcttatttt acttcgatgc ttttagatga
     1801 aggcgctgat ggaatggatg ctctgcagt
//
LOCUS       DASWBY010000244         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_647220, whole genome shotgun sequence.
ACCESSION   DASWBY010000244 DASWBY010000000
VERSION     DASWBY010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647220"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VF870_04455"
     CDS             complement(<1..550)
                     /locus_tag="VF870_04455"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX9251467.1"
                     /translation="MLRKLILPIQIIIILNTVSFAQGGGLIIGNNFRIFPGSTDQTEV
                     FIVKHPNDNNTLFSSANTIRFQPNFFVSEGVYVTTDAGNTWFGSDTCKGANIFFNGGD
                     PGIAIDKNGTFILTRKGTTTFQGIYSHYSTDLGITWSTQNTISTEEIERATIVSDNDP
                     NSAYYGRTYACWAKLVPNYTIGF"
     gene            complement(568..2697)
                     /locus_tag="VF870_04460"
     CDS             complement(568..2697)
                     /locus_tag="VF870_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9251468.1"
                     /translation="MSFLIAIFCSNIIAQKKDSVLYLIPIDQIDSIKYQTDEVIVTGT
                     RSDQKIIDIPYSVYRISPVQYKFDRKNSISDVLNTIPGVFMQSRYGNHDVRISIRGFG
                     SRSNTGIRGVRILLDGIPESEPDGQTRIEAIDFNAIGSTEIVKGNSSSLYTNAPGGVI
                     NFINDINFSKSFVSQHNEFGAFELRNNGLKVGIRTDKYGFMNTYTYHTYQGYRPHSED
                     YWHILNTVLESMPGDNTNLQFLGYLATGMIRLPGSLTKEEFERDPFQAAQQEVNRDVK
                     RISKKGRAGIRFTSKFGSSLNNDIEVIGYGTIKYFERTARTYRIFDRYGLGGSVKYTN
                     RSVIFDRENTLTLGGDLFYQTGPISEYNNINGLKGDNLISQIDETVGNTGLYFLDNIE
                     LYNKQLFFLFSGRYDNVYFDNVDRLNASRNDQLRYDDFTPKFALNFKFTPAVAVFTSF
                     GYSFDSPAGNELDDYPRPDRPAGMLNQDLEPQKSTNFELGLKGNLLNEQSNFFRNSFF
                     ELTFFNTTVENEIVPFEVYGDVFYRNSAKTNRMGIEAGFNTEIFNGLKSVLSYTFSNF
                     KYDEYSAVSIQLDSTGQISETAEDFSGNIVPSVPKHNFVFSLEYQHQISQNFVGFIKG
                     TYQNISGMFVNDANSDQTEAYQVLNGNLGVEFTKGGFNVLVSGGVNNIFDKTYVGFIN
                     INSANGRFYEAGEPQAFYATLKLGYQF"
BASE COUNT          918 a          501 c          405 g          951 t
ORIGIN      
        1 taaatccaat agtgtaatta ggaacaagtt ttgcccaaca cgcatatgtt cttccatagt
       61 aagcactatt tgggtcatta tccgagacaa ttgttgccct ttcaatctct tctgttgaaa
      121 ttgtattttg agttgaccag gtgattccca aatcagtaga ataatgagaa tagatacctt
      181 gaaatgtagt ggtccctttt cttgtaagaa taaacgttcc atttttatct attgcaattc
      241 caggatcacc accattaaaa aaaatatttg ctcctttaca agtatctgat ccaaaccagg
      301 tattacctgc atcagtagtt acataaactc cttcacttac aaaaaaatta ggttgaaatc
      361 ggatggtatt tgctgatgaa aataaagtat tattatcatt gggatgttta acaataaaaa
      421 cttcagtctg atctgtactt ccaggaaata ttctaaaatt atttccaata attaaaccgc
      481 ctccttgtgc aaaggagacg gtattaagga taattataat ttggatcgga agaattaatt
      541 ttcttagcat tatactttct tataaactta aaattgataa cctaacttta aagtagcata
      601 aaatgcttgt ggttcacccg cttcatagaa tctaccatta gcagaattta tattaataaa
      661 tcccacataa gttttatcaa aaatattatt tactcctccg gaaacaagta cattaaatcc
      721 accctttgta aattcaactc caagattgcc atttaatact tgatatgctt ccgtttgatc
      781 ggagttagca tcattaacaa acatgccgct aatattctgg taggtacctt ttataaatcc
      841 gacaaagttt tggctaatct ggtgttgata ttccaaagag aaaacaaaat tatgttttgg
      901 aacgctgggg actatatttc cactaaagtc ttctgctgtt tcagaaattt gtccggtaga
      961 atctaactga atagaaactg ctgaatattc atcatattta aagtttgaga aagtgtaaga
     1021 taacacagac tttaatccgt taaatatttc tgtattaaaa ccggcttcga ttcccattct
     1081 atttgttttt gcagagtttc tataaaatac atcaccatat acttcaaatg gtacaatttc
     1141 attttctaca gtcgtgttaa agaatgttaa ctcaaaaaaa ctatttctaa agaaattact
     1201 ttgctcatta agcagattac cttttaaacc taattcgaag ttagttgatt tttggggttc
     1261 caagtcctgg tttaacattc cagctggtct gtcaggtctg ggataatcat ccaattcatt
     1321 tccggcaggc gaatcaaaac tatatccaaa tgaagtaaag actgctactg cgggagtgaa
     1381 tttaaagttt aaagcaaact ttggagtaaa atcatcatat cttaattgat cgtttctaga
     1441 agcatttaat ctgtctacat tatcaaaata aacattatca tatctgccgc tgaacaaaaa
     1501 gaataattgc ttgttataca attcgatatt atcaaggaaa tataaaccag tgttgccaac
     1561 cgtttcgtca atttggctga ttaaattatc cccctttaat ccgtttatat tgttatactc
     1621 ggatattggt ccggtttgat agaatagatc accacctaag gttaaagtat tttcgcgatc
     1681 aaatattacg cttctgtttg tgtattttac agaaccgcca agcccatatc tatcaaatat
     1741 cctataagtt cttgctgttc tttcaaaata cttaattgtt ccatacccaa taacttcaat
     1801 atcattatta agagatgaac caaatttaga ggtaaatctt attcctgccc tgcctttttt
     1861 agaaatgcgc ttcacatcac gattaacttc ctgttgtgca gcttgaaatg gatctcgttc
     1921 aaattcttct tttgttaatg aaccaggcag tcttatcatt cctgttgcca gatagccaag
     1981 aaattgtaaa ttggtattat cacccggcat tgactcaaga actgtattaa gtatgtgcca
     2041 ataatcttca ctatgcggtc tatagccttg ataagtatga tatgtgtaag tattcataaa
     2101 accatattta tcagttctga ttccaacttt taatccatta tttcttaact caaaagctcc
     2161 aaattcatta tgctgcgaaa cgaacgattt tgagaaattt atgtcattaa taaagttgat
     2221 aactcctcca ggggcatttg tatataagga tgatgaattc cctttaacga tttctgtgct
     2281 tccaattgca ttgaaatcga ttgcctcaat tcttgtctgt ccatcgggtt cagattcagg
     2341 aatcccatca agtaaaattc ttactccacg aatacctgta tttgatcggc ttccaaaacc
     2401 gcgaattgaa attctaacat catgattacc ataacgcgac tgcataaata caccagggat
     2461 agtattcaat acatcgctta tagaattttt tctatcaaat ttatattgta caggacttat
     2521 tctataaacc gaataaggaa tatcaataat tttttgatct gacctggttc ctgtcactat
     2581 tacttcatca gtctgatact ttatagaatc aatctgatca atcggaatga gatacaaaac
     2641 tgagtccttt ttttgtgcaa taatatttga acaaaatatt gcaattaaaa aagacatcaa
     2701 tataaacctc ttaaaaagat tcataaatgt gacctttcaa aatttgtgta atactcaaga
     2761 gaagacaatt aataa
//
LOCUS       DASWBY010000245         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_271467, whole genome shotgun sequence.
ACCESSION   DASWBY010000245 DASWBY010000000
VERSION     DASWBY010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271467"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..>1666
                     /locus_tag="VF870_04465"
     CDS             26..>1666
                     /locus_tag="VF870_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX9251469.1"
                     /translation="MLSYAITPDKDYFQLITDKTNVVRPGKKSDEAILYDKQKVIDEL
                     GFEPKYMVDYLALVGDSSDDIPGVAGIGPKGAVPLIQKYGTIENIYKHIDEIDKAGIK
                     KKLEASRENAFLSKDLATIHCEVPIDFDFEHAKFTDPDFEKIKEILFELEFKAIYAKL
                     IKFYQTNSYEETEQVKESKEIKQEAEISFSNEAMNSFDDKKVKYQLIVKENEARKLAA
                     RLLESDLFVFDTETDGLNTFEVKLAGASFCIKPDEAYFIALNPFDDQPGLFENNLSDR
                     LPVSNFIKIFKKVFENDEIKKVCQNGKYDIAVLKSIGIEVNNFYFDTMLASYVLDADQ
                     KHGMDDLAEKYLNYSPIPISSLIGEKKDPTKIFEAEVNSLSNYAAEDADITYKLYEKL
                     DKELKKEGIGTIAYDIEFPLVPVLGDMEYEGIKIDIEALRQFSKDLQILIDNYTAEIF
                     KVAGTEFNINSPKQLQEILFTKLNLATGRKTKTGFSTDARSLENLRGEHEIIDMILNY
                     RQASKLKSTYADALPNIINKKTGRIHTSFNQTVASTGRL"
BASE COUNT          617 a          241 c          306 g          502 t
ORIGIN      
        1 agtaaaaaag gcagaagaat taggaatgtt atcttacgca ataacacctg ataaagatta
       61 ctttcaatta ataaccgata aaacaaatgt tgtaagaccc ggcaagaagt ccgatgaagc
      121 aattctatat gacaaacaaa aagttattga tgaactcggt ttcgaaccaa agtatatggt
      181 tgattatctt gctttagttg gagacagcag tgatgacatt cccggtgttg cagggattgg
      241 tccaaaaggc gcagtgccat tgatccaaaa gtatgggacc attgaaaata tctacaaaca
      301 tattgatgag atagataaag ccggaatcaa gaagaaatta gaagcaagca gagaaaatgc
      361 ttttctttca aaagatcttg ctacaattca ttgtgaagtt ccaatagatt ttgattttga
      421 acatgcaaaa tttactgatc cggattttga aaaaataaaa gaaatcttat ttgaacttga
      481 attcaaagcc atttacgcaa agttgattaa gttttatcag acaaattctt atgaggaaac
      541 agaacaagtt aaagaatcaa aagagattaa acaggaagca gaaatttctt tttcaaatga
      601 ggcaatgaat tcttttgatg ataaaaaagt taagtaccaa ttaattgtaa aagagaacga
      661 agcaaggaaa cttgctgcac gattactaga atcagattta tttgtttttg atactgaaac
      721 agatggactt aatacatttg aagttaaact tgctggtgct tccttctgta taaaaccgga
      781 tgaagcatat tttattgctt taaatccatt cgatgatcag cctggattat ttgagaataa
      841 tttatctgat cgattaccgg tttcaaattt tatcaaaatt tttaaaaagg tatttgagaa
      901 tgatgagata aaaaaagtct gccagaatgg taaatatgat attgctgttt taaaaagcat
      961 cggaattgag gttaataatt tttatttcga tactatgctt gcaagctacg tacttgatgc
     1021 ggatcagaaa catgggatgg atgaccttgc tgaaaaatat ctcaactact cccctattcc
     1081 aatttcgagt ttaattggag agaaaaaaga tccaacaaaa atatttgagg cggaagtcaa
     1141 ctctctttca aattatgctg ctgaagatgc agacataact tacaaacttt atgaaaaact
     1201 tgataaagaa ttaaagaaag aaggcattgg aacaatagct tatgatatcg agttccctct
     1261 ggtgcctgta ttgggagata tggaatatga agggatcaag attgatattg aagccttaag
     1321 acagtttagt aaagatcttc agattttgat tgataattat actgctgaaa tatttaaagt
     1381 tgcaggtact gaatttaaca tcaattctcc aaaacagctt caggaaattt tatttacaaa
     1441 acttaatctt gcaacgggta gaaaaactaa aaccggtttt tctactgatg cgcgttcgtt
     1501 ggaaaatttg cgcggcgaac atgagattat tgatatgata cttaattacc gtcaagcatc
     1561 aaaactaaaa tctacttatg cggatgcact gcctaatatc ataaataaaa aaacaggacg
     1621 tattcataca agttttaatc aaactgttgc atctactggc aggctt
//
LOCUS       DASWBY010000246         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_196077, whole genome shotgun sequence.
ACCESSION   DASWBY010000246 DASWBY010000000
VERSION     DASWBY010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196077"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1486
                     /locus_tag="VF870_04470"
     CDS             143..1486
                     /locus_tag="VF870_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251470.1"
                     /translation="MRSVLLITFLFILSSQSYTQWDKYPRPDEGVMTGGMGINWIDGQ
                     PYYRVSFRPEVSFANFGIGLDLNLDFDSQGKLRTENFNETSDYLSIIRYVRYGVKNDP
                     IFIKVGALDYYTLGHGSIMQHYNNSPTYDNRRIGLVFDLDLGKFGIESIYSSFSPQGV
                     VAVRGYVRPLQLTSATDLPIISNLEVGASFASDMNKDAGILNGTYNHATQEFNVTEDL
                     GSLSIYGFDLGLPLLNSSVTDITLYVDYAKIIDFGSGVATGVKFDLDASSLLNISAKF
                     ERRFNQGKYVPAYFNALHEINRFQVDSATGTFTSKAKLLSEFDEDTKGWYGDLLIRVL
                     NLFDIYGSYQRLDKYPKSGELNLRAAFEPEGAPIVLRAGYDKINIQDEKDAFTLDDRS
                     YLFVEAGYKPVEYILVSLLYSWTYTPIRGSDDRIISFEPQKRIEPRISFIYPFKF"
     gene            complement(1524..2168)
                     /locus_tag="VF870_04475"
     CDS             complement(1524..2168)
                     /locus_tag="VF870_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9251471.1"
                     /translation="MESRFKGIIFDVDGTLTFTNQLIFDSFNHITKKYLGRTYSDNEI
                     IALFGPTEDVILKEMCKEEYESARKDYYAYYKDNHDIARLYDGIKELIIDINKAGILL
                     SIFTGKGRTSALITLEELGLTDYFDMIVSGDDVENHKPSPEGIIMFLNKHNFNPKEVL
                     MIGDAPSDIIAANQCGVEIASVVWDSYAEDEVKKLNPKNLFHSVEELREYIFNI"
     gene            complement(2175..3308)
                     /locus_tag="VF870_04480"
     CDS             complement(2175..3308)
                     /locus_tag="VF870_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9251472.1"
                     /translation="MKPQMSIKILVGLLLLVNINVSAYKPINVFAIIDSLVQVQDSSL
                     QKVWEINNGSFSSTKLLVSPNFIYSTSDSGLVTCYNYDGSEKWAAEVLGMITNNSALY
                     KDLLLTSTQEGDLYSINSNNGEILQVVGIGENITTDLSLIEITNDNSKSIGVILGTSE
                     GNIFCYDAFTFELLWQKNISSSKLISTPIVEKDKLVFLNGDFSLYCVNAKSGSLIWKY
                     EFSDDQNNSAINFPVSNGKNIFSLSPDGKLFAIDLLLGKKSWSSNTKEMLNQIYVTSD
                     KQKLFLFDNKGFMAVYSAKDGKEISKADFKKSDLFAFTIAENQENTFIGSSDGSLFTF
                     DSKFVTRELLSPNQIPITSINVINEYEFIVKDKNGIITFYKIN"
     gene            3553..>3755
                     /locus_tag="VF870_04485"
     CDS             3553..>3755
                     /locus_tag="VF870_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX9251473.1"
                     /translation="MDIVALNEKIKNESAFIDILMNEISKVIVGQKVMVERLIVGLLG
                     NGHVLLEGVPGLAKTLAIKTLATA"
BASE COUNT         1222 a          576 c          636 g         1321 t
ORIGIN      
        1 gaatctatca attatatcta gattttcgtg agttttcata aggtatatat tttgttagat
       61 tcgaatcaaa aaaaatattg ttactaaaag aataactttt gtaaaataga attaattcta
      121 atccaaatcg acagaggtaa aaatgagatc agtactatta attacttttt tatttatttt
      181 atcatctcaa tcatatacac aatgggataa atatccacgt cctgatgaag gtgttatgac
      241 aggtggaatg ggaattaact ggattgatgg ccagccttat tatcgtgtaa gtttcagacc
      301 tgaagtttcc tttgcaaatt ttggaattgg tttggatcta aatcttgatt ttgattctca
      361 gggaaaactt agaacggaaa attttaatga gacttcagat tatttaagta taatcagata
      421 tgttaggtac ggagtaaaaa atgatcctat ttttattaag gttggtgcat tagactatta
      481 tactcttggt cacgggagta taatgcagca ctacaataat agtcctactt atgataacag
      541 aagaatcgga ttagtgtttg atcttgatct tggaaaattt ggtatagaat cgatttacag
      601 ttcgttctcg cctcaaggcg tggttgcggt tcgcggttat gttcgcccgc ttcagcttac
      661 ctctgcaacc gatctgccaa taattagtaa tcttgaagtt ggtgcttcat ttgcatcaga
      721 tatgaataag gatgcaggaa ttcttaacgg tacatataat catgcaacgc aagagttcaa
      781 tgtaacagag gatttaggat cattaagtat ttatggattt gatttaggtt taccgttgct
      841 aaattccagc gtaaccgata ttacattgta tgttgattat gcaaagatta ttgattttgg
      901 aagcggagta gcaacaggtg ttaaatttga tcttgatgcc agctctttat taaacatttc
      961 tgcaaagttt gaaagaagat ttaatcaagg taagtatgta ccggcttatt ttaatgcgct
     1021 gcatgaaata aatagatttc aggttgattc agcaacagga acttttacca gtaaagccaa
     1081 acttctttca gagttcgatg aggacactaa aggatggtat ggtgatcttt taataagagt
     1141 gttaaatttg tttgatattt acggcagcta tcaaagatta gataaatacc ccaaaagcgg
     1201 tgagttaaat ttaagagcag catttgaacc agaaggtgcg ccgatagttt tgagagccgg
     1261 atatgataaa ataaatatac aggatgagaa ggatgctttt acacttgatg acagatcata
     1321 tttatttgta gaggctggct acaaacctgt tgagtatatt ttagtctcat tactttatag
     1381 ctggacttac actcctattc gtggtagtga tgatagaatt atttcttttg aaccgcagaa
     1441 gagaatagag ccaagaattt cgtttattta tccttttaaa ttttaactaa taaaaaaaat
     1501 gggaacggtc gggataccgt tccctaaata ttaaaaatat attctcttaa ctcttcaact
     1561 gagtgaaaaa gatttttagg atttaatttt tttacctcat cttccgcata actatcccaa
     1621 acaaccgagg caatttcaac accacattga ttagctgcaa taatatctga tggggcgtct
     1681 ccaatcatca gcacttcttt cggattgaaa ttatgtttat tcagaaacat tattattcct
     1741 tccggagatg gcttgtggtt ttctacatcg tctccgctta caatcatatc gaagtaatca
     1801 gttaatccaa gctcttccag agttatcagt gctgatgttc tgcctttgcc ggtaaatatt
     1861 gaaagcaata tcccagcttt atttatatct attataagtt ctttaattcc atcatataat
     1921 cgagctatat catgattgtc tttatagtaa gcataataat ctttgcgtgc gctctcatat
     1981 tcctctttgc acatctcttt tagaataaca tcttcagtcg gaccaaataa tgcgataatc
     2041 tcattatcgg aataagttct tccaagatac ttttttgtga tgtgattgaa tgaatcaaaa
     2101 ataagttgat ttgtaaatgt taaagtacca tcaacatcaa atatgatccc tttaaatctt
     2161 gattccattt tttcttaatt gattttgtaa aatgtaatta ttccattctt gtcttttacg
     2221 ataaattcat attcgttaat tacatttatt gaagttattg gaatttggtt tggcgataat
     2281 aattctctgg taacaaattt agaatcaaat gtgaacagtg agccgtctga agatccgata
     2341 aaagtatttt cttgattttc tgctattgtg aaagcgaaca aatctgattt cttaaaatcg
     2401 gctttactaa tttcttttcc gtcttttgca gaatagactg ccataaagcc tttattatcg
     2461 aataaaaata atttttgttt atctgaagta acatagattt gatttaacat ttccttagtg
     2521 tttgatgacc aggacttttt cccaagcaaa aggtcaatcg caaatagttt tccatccggt
     2581 gacaatgaaa aaatattttt accattactt acaggaaaat ttattgcaga attattttga
     2641 tcatctgaga attcatactt ccagattaaa gaaccggatt ttgcattaac acaataaaga
     2701 gaaaaatcac catttaaaaa cactaattta tctttttcaa ctattggtgt tgaaattaat
     2761 tttgaactgg aaatattttt ttgccatagt aattcgaatg taaatgcatc ataacaaaaa
     2821 atattacctt ctgaagttcc gagtatgacc ccaatacttt tagagttatc atttgtaatt
     2881 tcaatcagcg aaagatcagt tgttatattt tcaccgattc caacaacctg taaaatttct
     2941 ccgttattag agtttattga atataaatca ccctcctgcg tagaagtcaa aagtaaatct
     3001 ttataaagtg ccgagttgtt tgtaatcatt cccaaaactt ccgctgccca tttttcactc
     3061 ccgtcataat tatagcaagt aacaagtccg ctgtctgatg tgctgtatat aaagttggga
     3121 gagactaaaa gttttgttga actgaagctg ccgttgttta tttcccaaac tttttgaaga
     3181 gaactatcct gtacctgaac aagggaatct ataattgcga aaacattaat tggtttataa
     3241 gctgagacgt taatgttaac taataatagt aacccgacta atatttttat gctcatttgc
     3301 ggtttcattt atttatatcc tcttaaaaaa attagaagac aatcaaaagg attattcctg
     3361 gcaaatgaaa tttaataaaa tttaggtaat ggtattttga gtctgttttt ttgattaagg
     3421 atttgccaaa tattgagcaa gttaatgcaa ataaatttcc ttgcattaaa aatgcaacct
     3481 ttatatgttt ggcggcgtct aatcgaatta aattaaagaa ttttagtatt taataaatag
     3541 aaaggtttta ccttggatat tgttgcatta aatgaaaaaa ttaaaaacga aagtgctttc
     3601 attgatatac tgatgaatga aatcagtaaa gttattgttg gtcaaaaagt aatggttgaa
     3661 cgattgatag ttggactgct tggcaacgga cacgttttac ttgaaggcgt tcccggtctt
     3721 gcaaaaactc ttgccatcaa aaccttagct acagc
//
LOCUS       DASWBY010000247         5045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_609704, whole genome shotgun sequence.
ACCESSION   DASWBY010000247 DASWBY010000000
VERSION     DASWBY010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609704"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="VF870_04490"
     CDS             complement(<1..1398)
                     /locus_tag="VF870_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9251474.1"
                     /translation="MIKKFLTIILFAFSLYAQQSPMDQNQFMLAESYEQKGDLNKAVE
                     IIETLNKKDPANIQYFNKLNTLYLQLKKYDESAALINSRINITPQDISLYGLLGSTYY
                     TAGDRTNAYQVWDSATEKFKTNPMTFRIIANYTIEKRDFEKAIEYLNHGKEISKDPFL
                     YSYDLGELYQITMRYREAAKEYCDLIKANPSQYQQIESKILSYANKPNALDETIEVVA
                     KYKSDNISFSYLLARLYIEKKNYDEAFDLYKDIDQKQNTKGADLFSFGDFVYQDGEYK
                     TASEVYKFLIDNYNDQQNISLAKLGFAKTQEALFIQKYNAANPEWKTFFIPAKIEEKE
                     VEPVISAYQEIIKVYQHSEVAIEANLRIGILLAHYRDDIINAEKYFSIITETYPMSKF
                     ASLAFIELGNIKMQQAKLDEAEKLFQSVIKLQRANPEDRSYANYQLARIFSFRNDFES
                     ARNNLLTVMGNLKDNI"
     gene            complement(1522..1881)
                     /locus_tag="VF870_04495"
     CDS             complement(1522..1881)
                     /locus_tag="VF870_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX9251475.1"
                     /translation="MKNTIYSLLLFFLFFGASGFAQEEKLDKYPEPIGGIEAMIKNVV
                     YPVSAKEAGVEGKVFVKAIIDEQGNVTETSIQKSVNEDCDKAAMDAIKKTKFTPGIKD
                     NKPVKAEVVIPVMFKLS"
     gene            complement(1906..2640)
                     /locus_tag="VF870_04500"
     CDS             complement(1906..2640)
                     /locus_tag="VF870_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX9251476.1"
                     /translation="MKTKLFLLILLFLIPFGCKEEKQIEIIPDYDRIYLPTNKLDEIP
                     QLLEGDSQKLLDSVMNTYVKHYPISDTLKSKPTMEYKFMISETGKIEKIFLGKNNDKS
                     IDELVLNTVKNWKYKPGVKDGKNVKSQMPMILWLESNNSINENNFATVVENMPGPIGG
                     MYAIQEKIRYPEIAKLAGIQGRVIIQAFIDESGSVVHAKVLNGIGGGCDEMAIDAVKQ
                     TKFNPGKQNGKPVKVQVTIPILFKLQ"
     gene            complement(2648..3115)
                     /locus_tag="VF870_04505"
     CDS             complement(2648..3115)
                     /locus_tag="VF870_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251477.1"
                     /translation="MSENKLLEMINLYFDGELEKSKEVYLITLLASHQTARDYFKQLS
                     VIRNAVDNSAEDFPAELEERILRSVGSKASKKTGIFSKVKTFSAISYAAALILLFLSS
                     YLFFKVSNYQERVDNLSEQMMMQTKTIQMLYNSLPGIEVRATYDNEIIIKPNS"
     gene            complement(3237..3761)
                     /locus_tag="VF870_04510"
     CDS             complement(3237..3761)
                     /locus_tag="VF870_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9251478.1"
                     /translation="MQQKENQIIEFTILFNKYKKRIYNYALKMLNDKMRADDIVQDVF
                     IKLFENLNNIHNKQSIQFWLFKTARNEMMSFLRNTKNKKFITESVDIENFEIENKTSL
                     ADEIENTELKKLILNELEMMNEDFREVFVLKEYSGLSYKEIASLLEIDEELVKSRLYK
                     ARQKLIDKISKLVE"
     gene            3946..5045
                     /locus_tag="VF870_04515"
                     /pseudo
     CDS             3946..5045
                     /locus_tag="VF870_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
BASE COUNT         1504 a          878 c          808 g         1855 t
ORIGIN      
        1 gatattgtct tttagattgc ccatcaccgt taacagatta tttcgtgctg actcaaaatc
       61 atttctaaac gaaaatattc tagcaagttg ataatttgca taactcctgt cctcaggatt
      121 tgccctttgt agcttaataa ctgattgaaa aagtttttct gcttcatcca gttttgcctg
      181 ctgcatttta atattaccaa gttcaataaa tgcaagcgat gcaaactttg acatcgggta
      241 agtttcagta attatgctaa aatatttttc tgcattaata atatcatcac gataatgagc
      301 cagaagtatt cctattctta aatttgcttc tattgcaact tcggaatgct gatacacttt
      361 tatgatttcc tgatatgcgc ttatgacagg ttcaacttct ttttcttcaa tttttgcagg
      421 gatgaaaaat gttttccatt ccggatttgc tgcattatat ttttggataa aaagtgcttc
      481 ctgagttttt gcaaatccta actttgccag agaaatattt tgctgatcgt tgtaattatc
      541 tataagaaac ttgtaaacct cagatgctgt tttatactcg ccgtcctggt aaacaaaatc
      601 accgaaggaa aaaagatctg cgccctttgt gttttgcttt tgatcaatat ccttgtaaag
      661 atcaaatgct tcatcgtaat tctttttctc gatatataat cttgcaagaa gataagaaaa
      721 acttatgttg tctgatttat attttgcgac tacttcaatc gtttcatcaa gagcatttgg
      781 tttgtttgcg tatgatagaa ttttactttc tatttgctga tactgcgatg gattggcctt
      841 gatcaaatca caatattcct tagccgcttc ccgatagcgc attgtaatct gatatagttc
      901 cccaagatca tacgagtata aaaaaggatc tttggaaatt tcttttccat gattcaaata
      961 ctcgatagct ttttcaaaat ctcttttttc aatagtataa ttagcaatga tgcgaaacgt
     1021 cattggattt gttttaaact tttcagttgc actatcccaa acctgataag catttgtgcg
     1081 atctccggca gtgtaataag tagatccgag taatccatat aaactaatat cctgcggagt
     1141 gatgtttatt cttgagttta taagcgcagc agattcatca tattttttta gctgaaggta
     1201 aagcgtatta agtttgttga agtactgaat atttgctgga tcctttttgt ttagagtttc
     1261 tattatttca acagctttat ttaaatcacc tttttgctca tagctttctg cgagcataaa
     1321 ttggttttga tccattgggc tttgctgagc ataaagcgaa aatgcaaata gaattattgt
     1381 aagaaatttc tttatcataa cttaaaaata gcaattaaat gcgggcattc aaggggagaa
     1441 aataataaca caccccttta actccccttc tcgatgaacc gggacaggct cgagaaggga
     1501 aaagaaggag tgtgtaaatt tttaagaaag cttaaacata acaggtatta caacttctgc
     1561 ttttaccggt ttgttatctt taattccggg agtgaatttt gttttcttta ttgcatccat
     1621 tgcagcttta tcacaatctt cattaacact tttctggatg gatgtttctg tcacatttcc
     1681 ctgttcatca attatggctt ttacaaaaac tttaccttca actcctgcct cttttgctga
     1741 aactggataa acaacatttt taatcattgc ttcaattcca ccaattggtt ctggatattt
     1801 atctaacttt tcttcctgag cgaatccgct tgcaccaaag aagagaaaga aaagtaaaag
     1861 tgaataaatt gtgtttttca tattgcctcc ttttttgatt tatgtttact gaagtttaaa
     1921 taatattgga atagttactt gtacttttac tggtttacca ttctgctttc cgggattaaa
     1981 cttggtctgt ttaacagcat caatcgccat ttcatcacac ccaccaccaa ttccgtttaa
     2041 tacttttgca tgtacaacgc tgccgctctc atcgataaaa gcctgaataa ttactcttcc
     2101 ttgtattcct gctagtttgg caatctcggg atatcgaatt ttttcttgaa tcgcatacat
     2161 cccgccaatt ggtcctggca tattttcaac aactgtagca aaattatttt cattaattga
     2221 gttattactt tctaaccaca atatcatcgg catctgtgac tttacgtttt taccatcttt
     2281 tacccctggt ttatatttcc aatttttaac cgtgtttaat actaactcat cgattgactt
     2341 atcattattt ttacccaaga atatcttttc aattttgccc gtttcactta tcatgaattt
     2401 atattccatt gtaggtttgc tttttaatgt gtctgaaatt ggataatgct tcacgtaagt
     2461 attcataaca ctatctaata atttttgcga atcaccctcc aatagttgag gaatttcatc
     2521 aagtttattt gttggcagat aaattctatc ataatcagga ataatctcaa tttgcttttc
     2581 ttccttacac ccaaaaggaa ttaggaaaag aagaattaat aaaaataatt tcgttttcat
     2641 tttaacctca gctatttggt tttataataa tttcattatc gtaagttgcg cgcacttcta
     2701 ttccgggtaa actgttatac agcatttgta ttgtttttgt ctgcatcatc atttgttcgg
     2761 aaaggttatc aacccgttcc tggtaattgg atactttaaa gaaaagatag ctgcttaaaa
     2821 acagcaggat taaagcagcg gcataagaaa ttgcagaaaa ggttttcact ttagaaaata
     2881 ttcctgtttt tttagatgct ttcgatccaa cagatcttaa aattctttct tccagctcag
     2941 caggaaaatc ttctgcggag ttatcaacag catttcttat aacactaagc tgtttaaaat
     3001 aatctcttgc tgtctggtgg cttgcaagca gagtaatgag atatacttct ttgctttttt
     3061 caagctcacc atcaaagtag agattgatca tttctaataa tttgttttcg ctcatatgtt
     3121 ttatccttct atgattgtca ttgcgagtcc tgattaagga cgaagcaatc tgttaaattg
     3181 ttagattgct tcgctctccc gacacgtcgg gatcgctcgc aatgacgcta atacatttat
     3241 tccactagtt ttgaaatctt atctattaat ttctgccggg ctttgtacaa cctgcttttt
     3301 acaagttctt catcaatctc aagcaaagaa gcaatttctt tatagcttaa tcccgaatat
     3361 tcttttaaca caaacacttc tctaaaatct tcattcatca tttcaagttc attcaagatc
     3421 agttttttta attccgtgtt ttctatttca tcagcgagcg aggttttatt ttcaatttca
     3481 aaattttcaa tatctaccga ttctgtaata aactttttgt tttttgtatt ccttaaaaag
     3541 ctcatcattt catttctggc tgttttgaac aaccagaatt gtatgctttg tttgttgtga
     3601 atattattaa gattttcaaa caactttatg aatacatcct gcacaatatc atctgcgcgc
     3661 attttatcgt taagcatttt taatgcgtaa ttgtaaatcc gcttcttgta tttattgaag
     3721 aggattgtga actcaattat ttgattttct ttttgctgca aaatcgattt ttgtttctta
     3781 tctaaaagat gctaaaaacg gataaaatgt ttccatactt acaaaatatt ttatcaagaa
     3841 tctcgctttt aataattatt agcgcatttc ctcatcacag caagcctaaa ttacattgct
     3901 aaacttctca ttctttggta cttttaagcg ctaaattcta agaaaatgaa gaaaatttta
     3961 attttaggtt cgtccggctc aattggtgtt aacactctta atgttataag gaattttccg
     4021 tataaatttg gtgttgttgg attaacagta aattcaagaa ttgatgtttt agaacagcaa
     4081 ataaaggaat ttaaaccgga atttgttgtt gttaccgatg aatctaaagc caaagaatta
     4141 caatctaata ttggcaattt gtgcaaagtt ctttccggtt atgatgaatt attaaatgca
     4201 gcttcgcaaa gtgattatga tatacttcta ggttctatgg ttggttttgc cggattagca
     4261 ccaactcttg aagccataaa acgtgggaaa cgaattgcgc tggcaaataa agaaactctt
     4321 gttgttgcag gtgaacttgt aacaaaactt gttttagaaa acagtgcgga aatacttccg
     4381 gttgattctg aacattcggc tatttatcag tgtttggtag gtgaaaatct taacgaagtt
     4441 gaaaagctaa ttttaacagc ttcaggcgga ccgtttttac acaaagataa atcgtttttt
     4501 gaaaatgcaa ctgttgatga agcgcttaat catcctaact ggaaaatggg aaataaaatt
     4561 accatagatt ccgctacgat gatgaataaa ggacttgaag taatcgaagc gcattggtta
     4621 tttggattgc ctgttgaaaa aattaatgtg gtcattcatc cgcaatcaat aattcattca
     4681 atggttcagt ttgttgatgg atcggtaaaa gcccagcttg gtttaccgga tatgaaattg
     4741 ccgatacaat atgcacttac ttttccggaa agattgcaga atgattttaa gagaacagat
     4801 ttaccggcaa taaataatct ctcttttttt gaaccggatt ttaataagtt tgaatgtttg
     4861 cagctggctt tgatgtaata aaatcgggcg gaactgcccc ttgtattttg aatgcagcaa
     4921 atgaaattgc cgttgacaag tttataaaca gacaaataaa attctctcag atttctgaat
     4981 tgattagtaa ggctctgaat aagattgaaa atcatattga tccggatatt gaaacaattt
     5041 ttgaa
//
LOCUS       DASWBY010000248         4137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_609732, whole genome shotgun sequence.
ACCESSION   DASWBY010000248 DASWBY010000000
VERSION     DASWBY010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609732"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VF870_04520"
     CDS             complement(<1..1078)
                     /locus_tag="VF870_04520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9251479.1"
                     /translation="MSNQIPACKNFTGYKPCFPDHNCWKDGCKENIPIGKNILIINFD
                     AMGDVLMTTAQLPAVKRKFPESKIFWVTLKIAAPLLYENHFIDQVFIYDAESISILNE
                     IEFDIVMNVDKSQRSCALLNSVKAKEKLGFGLDKNGVIIPVNQGAYYNYNLGMDDHFK
                     FKVNQRTGQDYLAETFELDYKRDDYTFNFTTEELRFIEEYKKDVRLRDTDKIIGFNTG
                     CSNLYPNKKMSVGQHIFLINKLLAKRKYKIMLLGGPEDTERNKEIADYFRGRIINTPT
                     NEGVRKGACYESIPRLIISGDSFGMHLAIALKKYVIAWFGVSCWSEIDLYDRGVKIYQ
                     EDLFCSPCWKKQCPYDLECIKNLDL"
     gene            complement(1053..2132)
                     /locus_tag="VF870_04525"
     CDS             complement(1053..2132)
                     /locus_tag="VF870_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9251480.1"
                     /translation="MKQFENWLKNILLKVLLIFNSSKNIKNQPVFNSDSNLLFIRLNR
                     IGDALVTTPLLYQIKQQIGCKIFVLADKKNYFVFSNNPSIDEVVIFEKGIKGFLQIKN
                     IISEKSIDAVVDLHDDVSTTVSFLIALANVKYKLGLRKSNSSIYTQTVKRLDSRTNHV
                     IDRILQIGLLFNLKFERKSIAINYIPSKEERIIAAERIKFMNPNKKFLIGVNISAGSE
                     ARFWGVDNYIKLSELLSNYDARVIFFTSKNESNFAKEITDEINIYPLTEAFGIFASAI
                     MQLDFLITPDTSVVHIASINKIPVFGLYVKYNTEDMIWSPYNTDFECVVTEEPTLKNI
                     SFDEVKNKLIPFLEKHLNVKSNSSL"
     gene            complement(2119..3135)
                     /locus_tag="VF870_04530"
     CDS             complement(2119..3135)
                     /locus_tag="VF870_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9251481.1"
                     /translation="MKINPFKIKKILCIKPRGIGDIVLSTIILENLIAHFPNAKIDYL
                     TEHFAKYSIKNNPLVNKVLTMHKAELVFKVAWRIRKEKYDLLIDLWSNPRSAQITFLS
                     GVKYRVGYAYRGRKYAYNILGTSDKGIHHSAEHNLELLKPLDVNIISKNIHYYIGNLE
                     KLFADDFFTENKLDAEKVFGIIPAGGWDSKRCDKEKWVEVCREAVNNFNCKILVLWGP
                     GDESDAEFIKSNLDDNCLLAPKSTLPQMASLISKCKLVIANDSGPMHISAALGVPTLG
                     IFGPTNPKAHGPYSLNSDYVIKDDLHCIICNKLICPYQHECMRDLEVKIVIEKIRALL
                     NETV"
     gene            complement(3193..>4137)
                     /locus_tag="VF870_04535"
     CDS             complement(3193..>4137)
                     /locus_tag="VF870_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX9251482.1"
                     /translation="NLRGEHEIIDMILNYRQASKLKSTYADALPNIINKKTGRIHTSF
                     NQTVASTGRLSSVDPNLQNIPIRTEMGKEIRRAFVPRDKNYKILSADYSQIELRIMAS
                     ICGDEALIKAFSEGEDIHTSTAALVFKVKPKDVTPDMRRKAKEVNFGILYGIGPFGLK
                     TRLGVTQTHAKEIIDTYFGTFKRVREFMDSSVRLAQEKGYAETLSGRRRYLKNINSNN
                     RVVRQFEERVAINMPIQGTAADMIKLAMINIHKELTKKKTKTKMILQVHDELIFDAHV
                     DEIDKLLPIIKKLMENALPLKVPVVAETGIGDNWLDAH"
BASE COUNT         1295 a          750 c          617 g         1475 t
ORIGIN      
        1 taagatcaag attttttata cattccaaat cataaggaca ttgttttttc cagcaaggtg
       61 aacaaaataa atcttcttga taaatcttta cgccgcgatc gtaaagatca atttcagacc
      121 agcagctaac accaaaccag gcaataacat atttctttaa tgcaattgcc agatgcatcc
      181 caaatgaatc accactaatt ataagtcggg ggattgattc atagcaagca ccctttctaa
      241 caccttcatt agtcggagta ttaattatcc tgcctctaaa gtaatcagca atctctttat
      301 tacgttcagt atcctcaggt ccgccaagaa gcataatctt atattttctt ttagccagca
      361 gcttattgat aagaaaaata tgctgaccaa cagacatctt tttatttgga tataaatttg
      421 aacagccggt gttaaaacca attattttat cagtatctct taagcggaca tcttttttgt
      481 attcttcaat aaaacgcagt tcttcggttg taaaattgaa agtgtagtca tcgcgtttat
      541 aatcaagttc aaaagtttct gcaagataat cttgtcctgt acgttgattt actttaaact
      601 taaaatgatc atccatccca agattgtaat tgtaatatgc tccttgatta actggaataa
      661 taactccgtt tttatccagc ccaaatccta atttttcctt agcttttact gaatttaata
      721 atgcacacga acgctgcgat ttatcaacat tcatcactat atcaaattca atttcattta
      781 atattgaaat tgattctgcg tcataaataa aaacctgatc tataaaatga ttttcataga
      841 gaagcggtgc ggcaattttt aaagtcaccc aaaagatttt tgattcagga aattttcttt
      901 ttactgccgg cagttgtgct gttgtcataa gcacatcacc catggcatca aagtttatta
      961 ttagaatatt cttgccgata ggaatgtttt ctttgcatcc atctttccag caattatgat
     1021 caggaaaaca gggtttgtaa ccagtaaaat ttttacaggc tggaatttga tttgacattt
     1081 aaatgtttct ccaaaaaggg tataagttta tttttaactt cgtcgaatga aatgtttttt
     1141 aatgttggtt cctctgtaac aacacattca aaatcagtat tatatggact ccaaatcata
     1201 tcttcagtat tgtatttaac gtataaacca aataccggta ttttatttat agatgcaata
     1261 tgaacaactg aagtgtccgg cgtaattaga aaatcaagtt gcataattgc tgatgcaaaa
     1321 ataccaaaag cttcggttaa aggataaatg ttaatttcat ctgtaatttc tttggcaaaa
     1381 tttgattcat tctttgatgt gaagaaaatt acacgagcat cataatttga taaaagttct
     1441 gataatttta tgtagttatc aactccccaa aacctggctt cgcttccggc agaaatattt
     1501 actccaataa gaaatttctt atttggattc atgaacttga ttcgttcggc tgcaattatt
     1561 cgttcttctt tcgaaggaat ataatttatt gcaattgatt tcctttcaaa ctttaaattg
     1621 aatagtaaac caatctgtaa aattcgatcg atcacatgat ttgtccgcga atctaacctt
     1681 ttaacagtct gggtgtaaat tgaagagttt gattttcgca atcctaattt atacttgaca
     1741 ttcgctaggg caattagaaa actaactgta gtggaaacat catcgtgcag gtctacaaca
     1801 gcatcaatag atttttccga tattatattt ttgatctgca aaaatccttt aattcctttt
     1861 tcaaaaatga ccacttcatc tattgaagga ttattgctaa agacaaaata atttttctta
     1921 tcggcaagca caaatatttt gcagccaatc tgctgtttaa tttggtaaag aagcggtgtg
     1981 gttaccaatg catcaccaat tctattaagg cggatgaaaa gcaaattaga atctgaatta
     2041 aataccggtt gattttttat attttttgat gaattaaaaa ttaaaagaac tttaagtaaa
     2101 atatttttta accagttctc aaactgtttc atttagcaga gccctaattt tttctattac
     2161 tatttttact tctaaatctc tcatacactc gtgctgataa ggacaaatta atttattaca
     2221 aattatacaa tgtaaatcat ctttaataac ataatcagag tttaatgaat acggcccgtg
     2281 agcttttgga tttgttggtc caaatattcc gagagttgga acgccaagtg cagctgaaat
     2341 gtgcattggg ccggaatcgt ttgcaattac aagtttacat ttagaaatca gagatgccat
     2401 ttgcggaagt gtagattttg gcgcaagcaa acaattatca tcaagattag atttaataaa
     2461 ttctgcatcg ctttcatcac cgggtcccca aagaactaaa attttacaat taaaattatt
     2521 gactgcttcc ctgcaaactt ctacccattt ttctttgtca catcgtttag aatcccatcc
     2581 gccggcggga attattccaa acactttttc agcatcgagt ttattctctg taaaaaaatc
     2641 atccgcaaaa agcttctcaa gattaccaat atagtaatgg atattttttg atatgatatt
     2701 cacatcgagc ggttttagca attctaaatt atgttcagca gaatgatgaa tacctttgtc
     2761 tgaagtccct aaaatgttgt atgcgtattt tctccctcta tacgcataac caactctgta
     2821 cttaacacct gatagaaaag ttatctgtgc acttcttgga tttgaccaaa gatctattaa
     2881 taaatcatat ttttctttgc ggattctcca ggcgacttta aatactaatt cagccttatg
     2941 cattgttaaa actttgttaa ccaacggatt attcttaatt gagtattttg caaaatgttc
     3001 agttaaataa tcaatcttgg cattcggaaa atgcgctatc aaattttcaa gaataattgt
     3061 tgataaaact atatcaccaa ttccacgcgg tttaatacag agtatctttt ttattttaaa
     3121 gggatttatt ttcatttaaa attattttga gcttaacttt atctagaact tattttgtcc
     3181 tcaccccgga ctctaatgtg catcaagcca gttatcacca atcccggttt ctgcaactac
     3241 aggaactttt aacggtaaag cattttccat caacttttta attatgggaa gtagtttatc
     3301 gatttcatcc acgtgcgcat caaaaattaa ttcatcatgc acctgcaaga tcattttagt
     3361 ttttgttttt ttctttgtaa gctctttatg aatgttaatc attgctaact taatcatatc
     3421 tgcggctgtt ccctgaatgg gcatatttat agcaacccgt tcttcaaatt gtcttacgac
     3481 tctgttattg ctgttaatat tttttagata tcttcttctt ccggataatg tttctgcata
     3541 acctttttcc tgggccaatc taacagaact atccataaat tctcgaacgc gtttaaaggt
     3601 accaaaataa gtatctatta tttctttagc gtgagtttga gtaactccta atcttgtctt
     3661 taaaccaaat ggaccaatac cgtaaagaat tccaaagtta acttctttag cttttcttct
     3721 catatcaggt gtaacatctt taggtttaac tttaaaaact aaagcagcag tacttgtgtg
     3781 aatatcttct ccttcgctaa aagcttttat caaagcttca tctccacaga ttgaagccat
     3841 aattctcaat tcaatctggc tgtaatcagc actcaatatt ttatagtttt tatcgcgggg
     3901 cacaaaagct ctgcgtattt cttttcccat ctcagttctg attgggatgt tttgcaagtt
     3961 gggatcaaca cttgaaagtc ttccggtaga agcaacagtt tggttaaagc ttgtatgaat
     4021 acgtcctgtt tttttattta taatattagg cagcgcatcc gcataagtgg attttagttt
     4081 tgatgcctga cggtaattca gaatcatatc aatgatctca tgttcgccac gcaaatt
//
LOCUS       DASWBY010000249         4233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_160485, whole genome shotgun sequence.
ACCESSION   DASWBY010000249 DASWBY010000000
VERSION     DASWBY010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160485"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1276
                     /gene="fabF"
                     /locus_tag="VF870_04540"
     CDS             20..1276
                     /gene="fabF"
                     /locus_tag="VF870_04540"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX9251483.1"
                     /translation="MSDKNRRVVVTGMGALTPIGLTVNEFWNGMMDCKSGAAMIKQFD
                     TSKIETKFACELKDFDPLNFLDKKTARRLDPFAQYALVSARQAVEDGNLQPEKLSEEE
                     KNRIGVIFGSGIGGIQTFYDQAVINREQGPGRISPFFIPMLIPDIAAGHISMQYGFRG
                     PNYCTVSACATGNNNMIDSFLLIKFGMADVMVAGGSEASISELGMGGFNASRALSTRN
                     DSPETASRPFDATRDGFVMGEGGGTVILEELEHALKRNAKIYCEIVGAGLSADAHHIT
                     APHPEGTGAILSMKMAIQQANVKPEEIDYVNMHGTSTPLGDIGETKAIKKVFGDYAYK
                     MNVSSTKSMTGHLLGAAGAVEAIASILAVVNDKIPPTINFANPDPDCDLNYTFNKPQD
                     RKVNYAISNAFGFGGHNTSVIFKKYE"
     gene            1295..1738
                     /locus_tag="VF870_04545"
     CDS             1295..1738
                     /locus_tag="VF870_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX9251484.1"
                     /translation="MKRIIYFSFILFISLSAAIFSSDGAKKRGNLIVKFNGLNSSEGN
                     VIIALCNSDENYMNHKSPFIGKSVPINNNNAIIEFDDLPFGEYAIKAFHDEDANNDLN
                     TNILGIPVEDYGFSNNARGMFGPPSWEDAKFVLSNDVKTIEIVIK"
     gene            complement(1929..2627)
                     /locus_tag="VF870_04550"
     CDS             complement(1929..2627)
                     /locus_tag="VF870_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251485.1"
                     /translation="MKLLISLLFILIQFGYSQSKDPNEIINAVITNFNKVNDYQVDVK
                     IKVDVEFIKVPETKAKIYFKQPDKVHLEAEGFAMLPKNGMEFSPSSFIKKDYTAVYEQ
                     DVDLNGFKTSIIKVIPLGNEGEVILSTLWVDQKNQVIRKVESTTKTNGTFTIDFTYDE
                     KIKYPLPVKIVFSFNMDKMNIPATISGETSTEKPDKKNKSSDSRTRGKVIVNYSNYLV
                     NKGIPDSIFEEKNK"
     gene            complement(2640..3479)
                     /locus_tag="VF870_04555"
     CDS             complement(2640..3479)
                     /locus_tag="VF870_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9251486.1"
                     /translation="MLTLIQIELYKIFKKWRTYIGFIAIGVLVPIIHTAMVFEGSHTL
                     NFMTRNIQESFVFVGNLLNGYLVSYLILTSLAVHIPFLIALVAGDLLAGEATAGTYRM
                     LITRPISRTQIITSKFLAGIIYTNLLILWLAIMSLVVGLIIFGTGELLVISNQTVIIF
                     EKSDVLWRFLLAYGFASLGMSVVASLAFLFSSLVENSIGPIVSTMAVIIVFIILSAIN
                     IDVFQTIKPYLFTNYILSWRLFFDDPVDFAELTKATLILVGHIVVFFSLTLFLFKRKD
                     ILT"
     gene            complement(3479..>4233)
                     /locus_tag="VF870_04560"
     CDS             complement(3479..>4233)
                     /locus_tag="VF870_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9251487.1"
                     /translation="KPTEGSIKLFNKTLEKNRIEILKRIGAIVEKHDFYGYLSAYKNL
                     EILGRISGKEISKNRIMEVLELVGLSKRYKSKVKTFSHGMKQRLGLGQALLHNPDLII
                     LDEPTTGLDPQGMKEIRDLILHLSKVEKKTIFLSSHILYEVELVANRMIIINKGTKKV
                     EGYVKDLLNAKKLKVSFEVNDEEKTTSLLNLSDWIHKLEYQAKNVFMFSLENDEIADL
                     NKYLVENGIAVSAVIPTRSLEDYFLKITDEVA"
BASE COUNT         1418 a          698 c          712 g         1405 t
ORIGIN      
        1 accttaaaag aggatacaaa tgtctgataa aaacagaaga gtagttgtaa caggaatggg
       61 agctttaact ccgattggat taactgtaaa tgaattctgg aatggtatga tggattgcaa
      121 aagcggcgct gcgatgataa agcaatttga tacatcaaaa attgaaacaa aatttgcctg
      181 cgagttaaaa gactttgatc ctttaaattt tctggataag aaaactgcac gtagattaga
      241 tccatttgca cagtatgctt tggtctccgc tcgtcaggca gtagaagatg gaaacttgca
      301 gcctgaaaaa ctttctgagg aagagaaaaa cagaatcggt gtaatcttcg gaagtggtat
      361 tggcggtatt caaacttttt atgatcaggc agtaataaat cgtgaacaag ggcccggcag
      421 aatttcacct ttctttattc caatgcttat tcctgatatt gcagccggac acatttctat
      481 gcagtatggt ttccgtgggc caaattactg tactgtttcg gcttgtgcta caggaaataa
      541 taatatgatt gacagctttc tgcttataaa atttgggatg gctgatgtga tggttgcggg
      601 cggaagtgag gcttctatat cagaacttgg tatgggtgga tttaatgcaa gcagagcatt
      661 atctacaaga aatgattcac cagaaactgc aagccgtcct tttgatgcaa cgagagatgg
      721 ttttgttatg ggtgaaggag gcggaactgt aattcttgaa gaacttgaac acgcattaaa
      781 acgtaatgca aaaatttact gcgaaatagt cggtgcagga ttatctgctg atgctcatca
      841 cataactgct cctcacccgg aaggtactgg tgctatttta tcaatgaaaa tggctattca
      901 gcaagccaat gttaaacctg aagaaattga ctatgtaaat atgcacggaa cttctactcc
      961 acttggtgac attggtgaaa ctaaagcgat aaaaaaagtg tttggagatt atgcatataa
     1021 aatgaatgtg tcttcaacaa aaagtatgac gggacattta ttaggtgcag ccggtgcagt
     1081 tgaagcaata gcatcaattc ttgctgttgt aaatgataag attccaccaa ctattaactt
     1141 tgcaaatcct gaccctgatt gcgatctaaa ctatactttc aataaacctc aggatagaaa
     1201 agttaattat gcaattagta acgctttcgg atttggtggt cataatacat ctgtaatatt
     1261 taaaaagtat gagtaacaat tatcaggata gttagtgaaa cgcattattt atttttcatt
     1321 catattattt attagtcttt cagcagcaat tttttcttcg gatggagcaa aaaagagagg
     1381 aaacttaatt gtaaaattta acgggctgaa ttccagtgaa ggaaatgtca taattgcatt
     1441 gtgcaattct gatgaaaact atatgaacca taagtcacct ttcatcggga aatccgttcc
     1501 aattaataac aataatgcaa ttattgaatt tgatgatttg ccatttggtg aatatgcaat
     1561 caaagccttt catgatgaag atgctaataa tgatttgaac acaaacattc ttggaattcc
     1621 tgttgaagat tatggatttt caaataatgc tcgtggaatg tttggtccgc cttcgtggga
     1681 agatgcaaaa tttgtattaa gtaatgatgt aaaaacgatt gaaattgtaa ttaagtagaa
     1741 acaactagtc atcccaattt ttttatacaa ccgcaaaatg actttattcg cctcagcaaa
     1801 tccaataaat ttatgttcaa atagttaagc ttggcggcgg tataaagaat cctcagtact
     1861 ttaagataag ttagttttcc tcaaactaag atctgaacca ccgctgaatt ttaacctgct
     1921 tatttatcct atttattttt ctcttcaaaa attgaatccg ggataccttt attaaccaga
     1981 taatttgaat agtttacaat tactttgccc ctggttctag aatcagaact tttatttttt
     2041 ttatcgggtt tttcagttga ggtctcaccg cttattgtgg caggaatgtt catcttatcc
     2101 atattaaatg aaaaaacaat ttttaccgga agcggatatt ttattttttc atcataagta
     2161 aaatctatag tgaatgtgcc attagttttt gtagttgatt caacttttct gataacctga
     2221 tttttctgat ctacccataa tgttgaaaga attacctcac cttcgttacc gagaggaata
     2281 actttaataa ttgaagtttt aaacccattt aaatcaacat cttgctcata taccgcagta
     2341 taatctttct ttataaatga tgaaggagaa aattccattc catttttagg aagcattgca
     2401 aaaccttcag cttccaagtg aactttatca ggctgtttaa aataaatttt ggcttttgtt
     2461 tcaggtactt ttataaattc aacatcaact ttaatcttta catcaacctg gtaatcatta
     2521 actttattaa agtttgtaat tacagcattt atgatttcat tgggatcttt tgattgagag
     2581 tatccaaatt gaattagaat aaataataaa ctgattagta atttcatctt atcctcacgt
     2641 tatgttagaa tatctttgcg tttaaaaaga aaaagagtta aagaaaagaa tacaacaata
     2701 tgcccgacta aaattaaagt ggcttttgta agttcggcaa aatcaacagg atcatcaaag
     2761 aataaacgcc aggaaagaat ataatttgta aataagtatg gttttattgt ctggaaaaca
     2821 tcgatattta ttgcagataa aattataaat actattatta ctgccatcgt acttacaatt
     2881 ggcccaatag aattttcaac cagtgaagag aacaaaaaag caagacttgc aactacagac
     2941 attccaagag aagcaaaacc ataagccaga agaaatcgcc aaagtacatc acttttttca
     3001 aaaattatta ctgtttgatt gctgatgact aaaagttctc ctgttccaaa aattattaat
     3061 ccaacaacta aactcattat cgccagccag aggattaaaa gatttgtgta aataattcca
     3121 gctaaaaatt ttgatgttat tatctgagtt cttgaaattg gtcttgtaat caacattcta
     3181 tatgttccgg cagttgcttc gccagcgagt aaatcacctg cgaccaatgc aattaaaaat
     3241 gggatatgca cggcaagact tgttaagatc aaataagaga ctaaatatcc attcaataaa
     3301 tttcccacaa agacaaatga ttcttgaatg ttccgcgtca taaagtttag agtatgactt
     3361 ccttcaaaaa ccatggctgt gtgaattatt ggaactaaaa ctccaattgc tataaatcca
     3421 atataggttc gccatttctt aaaaatcttg tataattcaa tttggataag cgttaacatt
     3481 atgcaacctc atcagtaatt tttaagaaat aatcctcaag tgatcttgta ggaataacgg
     3541 cactaacagc aattccattt tcaacaagat atttattaag gtccgcaatt tcgtcgtttt
     3601 caagtgaaaa cataaagaca ttttttgctt gatactcaag tttatgtatc caatcagaca
     3661 ggtttaaaag acttgtagtt ttttcttcat catttacttc aaaagaaact ttaagctttt
     3721 tagcattaag caaatcctta acgtagcctt caactttttt tgtgccttta tttataatta
     3781 tcattctatt tgcaacaagt tcaacttcat aaagtatgtg cgaagaaaga aaaattgtct
     3841 ttttttcaac cttgctaaga tgcaaaataa gatcccggat ttccttcatt ccttgcggat
     3901 ctaaaccagt agtaggttca tctagaatta tcaaatcggg attgtgaagt aatgcttgtc
     3961 ctaatcccaa tcgctgcttc attccgtgtg aaaaagtttt aactttactt ttataacgtt
     4021 tgctcaaacc aacaagttca agcacttcca taattctgtt cttacttatt tctttacctg
     4081 aaattcttcc aagaatttca agatttttat aagctgataa ataaccgtag aaatcgtgtt
     4141 tttctacaat cgcaccaatt cttttaagaa tctctattcg atttttttca agagttttat
     4201 taaataattt tatgcttcct tcggttggtt taa
//
LOCUS       DASWBY010000250         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_45943, whole genome shotgun sequence.
ACCESSION   DASWBY010000250 DASWBY010000000
VERSION     DASWBY010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45943"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /gene="atpB"
                     /locus_tag="VF870_04565"
     CDS             <1..440
                     /gene="atpB"
                     /locus_tag="VF870_04565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9251488.1"
                     /translation="LGLIPFSATFTSNIAVTATLAIFSFIVIQLGGMRNNGAFGYFKG
                     LIPHGVPVFLLPIMIVVEFLGLLAKPFALAIRLFANMTAGHIVIYALISLIFVMQSVG
                     WAGLAIPMALFIYALEILVALLQAYIFTMLSSLFIGMAVHQDH"
     gene            481..699
                     /locus_tag="VF870_04570"
     CDS             481..699
                     /locus_tag="VF870_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX9251489.1"
                     /translation="MDFAYLAAGLGAGLTIFGGALGIGKLAAAAMDASGRQPEAAGAI
                     RTSMIIAAALIEGISLFALVICFILAGK"
     gene            760..1314
                     /gene="atpF"
                     /locus_tag="VF870_04575"
     CDS             760..1314
                     /gene="atpF"
                     /locus_tag="VF870_04575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX9251490.1"
                     /translation="MIANTTFAFIALVVSETEGGSAGLLSVNGGLAFWTTVTFILLLI
                     LLTKFAWKPILSALKQREDAIKDSLEQAEKAKDEAKQILAENQNSLAKAEDESKKIIE
                     QSRIFAENLKEQMIKESKEQAQKIISDATAEIDRKKNAAFDELKNQIAEISVQAAEKI
                     LKENLDADKNKKLVDKYISDISKN"
     gene            1317..1871
                     /gene="atpH"
                     /locus_tag="VF870_04580"
     CDS             1317..1871
                     /gene="atpH"
                     /locus_tag="VF870_04580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX9251491.1"
                     /translation="MASSKVANRYAQSFLDTSIEKNILDRVTKDFELVYQSLIKSPEL
                     LRAFKSPVIKTETKQSILAEIFGKLISKDSMDFLNFVILKGREELFADILEKFENLKD
                     EHIGAVKIDVTTAFIFTEDQKNQLKDKFESHLKKTARLTFKVDQNIIGGFVAKVGDTV
                     YNASMIHQLGLLKKQLLQEGITLN"
     gene            1902..3443
                     /gene="atpA"
                     /locus_tag="VF870_04585"
     CDS             1902..3443
                     /gene="atpA"
                     /locus_tag="VF870_04585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX9251492.1"
                     /translation="MAEVRPDEISAILRKQLSGFESEVDIYDVGTVLQVGDGIARVYG
                     LSQVMASELVEFPNNVFGMVLNLEEDSVGCVLFGDTTLVKEGDTVKRTKRVASMPVGD
                     AMLGRVITPLGLPIDGKGPINTSQFLPIERKALGVVQRQPVKEPLQTGIVAVDSMIPI
                     GRGQRELIIGDRQTGKTAVAVDAIISQKFTHTEEAKALGINPVYCIYVAVGQKESTVA
                     QVVAKLQETGAMEYTTVISAAASEPAPLQFIAPYSGATLGEYFRDNGKHALCIYDDLS
                     KQAQAYRELSLLLRRPPGREAYPGDVFYLHSRLLERASKLSDAEGGGSLTALPIIETQ
                     QGDVSAYIPTNVISITDGQIYLESNLFNAGIRPAINVGISVSRVGGNAQIKAMKKVAG
                     SLKLDLAQYRELEAFAKFGSDLDKSTQRTLAKGARLVELLKQGQYAPVPVEKQVVSIY
                     YGTKGFMDDIAVKDVKRFEKEVLEFIEVKYKQIFDSIKKDKDVSKETEELIKKAGKEF
                     LSTFK"
     gene            3469..>3982
                     /locus_tag="VF870_04590"
     CDS             3469..>3982
                     /locus_tag="VF870_04590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX9251493.1"
                     /translation="MATLRDIKLRIKGVKSTQQITKAMKMVAAAKLRRATESILNARP
                     YAKKISVLLSHLVNEDDKNSNPLFAQREVKNVAVVVVTADRGLCGAFNTNIIKEASRY
                     VEEDIKQKGLDYKLFCLGKKGSDYFSRRNYNIAATYPGIFSLIDYTTAQKLVDEIIPK
                     YLDGSIDKVII"
BASE COUNT         1339 a          592 c          804 g         1247 t
ORIGIN      
        1 ttttaggatt aattcctttt tcagcaacgt ttacaagtaa tatagctgtt acagccactt
       61 tggctatttt ttcttttatt gtaatacagc ttggcggtat gagaaacaat ggagcatttg
      121 gttactttaa gggattaatt cctcatgggg ttccggtttt cttattacca attatgattg
      181 tggttgaatt tttaggatta cttgcaaagc catttgcatt ggctatacgt ctttttgcaa
      241 atatgactgc agggcatatt gttatttatg cattaataag tttgattttc gttatgcaga
      301 gtgttggttg ggctggtctg gcaattccta tggctttgtt tatatacgct cttgaaattt
      361 tagttgccct gttacaggca tatattttta caatgctgtc ttccttgttt ataggaatgg
      421 cagttcatca ggatcattaa gataattact aacattaaat ctaattaaag gagaacatta
      481 atggactttg catatttagc tgccggttta ggtgctggat taacaatctt tggtggtgct
      541 ttaggcattg gcaaattagc tgctgctgca atggatgcaa gcggaagaca accagaagcc
      601 gccggtgcta tcagaacatc tatgatcatc gctgctgcgt tgattgaagg tatttcttta
      661 tttgcattgg ttatctgctt tatcttagcc ggtaagtaat tagttaaatg gagtgtaatt
      721 acactcccat tatttaaaaa taaacttaac aataagatta tgattgcaaa tactacattt
      781 gcttttattg ctttagttgt atctgaaaca gaaggcggca gtgccggatt gctttccgtt
      841 aatggcggat tggctttttg gacgactgtt acttttattc tgcttcttat tcttcttact
      901 aagtttgcct ggaagccgat tttatcagct ttaaagcaga gagaagatgc tattaaagat
      961 tctctggagc aagctgaaaa agccaaggat gaggcaaaac aaattctcgc agaaaatcag
     1021 aatagcttag caaaagctga ggatgaatct aaaaagatta tcgaacagag cagaattttt
     1081 gctgaaaacc ttaaagaaca gatgattaaa gaaagcaaag agcaagctca aaaaataatt
     1141 agcgatgcaa ctgctgaaat cgataggaag aaaaatgctg catttgatga gttaaaaaat
     1201 caaatagctg aaatttctgt tcaggctgct gaaaagattt tgaaagaaaa tctagatgca
     1261 gataaaaata aaaaacttgt tgataaatac ataagtgata tatctaaaaa ttaattatgg
     1321 cttcatcaaa agttgcaaat agatacgctc agtcttttct tgatacttct attgagaaaa
     1381 atattctgga tagagtaaca aaagattttg aattggttta tcaaagcctg atcaaaagtc
     1441 ctgaattact cagagcattc aaaagtcccg tgattaaaac agaaaccaag cagtcaatac
     1501 ttgctgagat ttttggaaag ttaatcagca aagacagcat ggattttttg aattttgtta
     1561 tcctaaaagg cagagaagaa ttatttgctg atattttaga gaaatttgaa aatttaaaag
     1621 atgagcacat tggtgctgtt aagattgatg ttacaacagc gtttattttt acagaagatc
     1681 aaaaaaatca gttaaaagat aagtttgaat cacatcttaa aaagacagct cgtctaactt
     1741 ttaaagttga tcagaatatt attggcggat ttgtagcaaa ggttggagat acagtttata
     1801 atgcttctat gattcatcaa cttggtttat tgaaaaaaca attattgcaa gaaggcatta
     1861 cattaaatta attcaaagga atttaataaa ggaaattaca aatggcagaa gttagacctg
     1921 atgaaatatc tgcgatatta agaaaacaac tttctggatt tgagagtgaa gttgatattt
     1981 atgatgtagg aaccgtactg caggttggtg atggtatagc ccgtgtttac ggactttctc
     2041 aagttatggc gagtgagtta gttgaatttc caaacaatgt ttttggaatg gtgcttaatc
     2101 ttgaagaaga tagtgttggc tgcgtattat ttggcgatac aactttagtt aaagaaggtg
     2161 atacggttaa gcgtacaaaa cgtgtagctt ctatgcctgt tggcgatgca atgcttggac
     2221 gtgttattac tccacttgga cttccaatag atggaaaagg accaataaat acatctcagt
     2281 ttttaccgat tgaaagaaaa gcccttggcg ttgttcaaag acagcctgta aaagaaccgt
     2341 tgcaaacagg aatagttgca gtcgattcaa tgatccccat tggaagagga caaagagaac
     2401 taattatcgg tgacagacaa acagggaaaa cagctgttgc agttgatgca atcataagcc
     2461 aaaaattcac tcatactgaa gaagcaaagg ctttaggtat aaatcctgtt tattgtattt
     2521 atgttgctgt tggacaaaaa gaatctacag tagcccaggt agttgctaaa ctacaagaaa
     2581 ctggtgcaat ggaatataca acagttatct ccgctgcagc ctctgaaccg gcacctcttc
     2641 aatttatcgc tccatattct ggtgcaactt taggcgaata ttttagagat aatggtaagc
     2701 acgcactttg tatttatgat gatctttcaa aacaagcgca agcttacaga gaattatctt
     2761 tattattgag aagacctccc ggacgcgaag cttatcctgg agatgtgttt tatcttcact
     2821 caagattgct tgagcgagct tcaaagctta gtgatgctga aggtggtgga agtttaactg
     2881 ctcttccaat tattgaaaca cagcaaggag acgtttcagc atacatccct actaacgtga
     2941 tatcaattac cgatggacag atttatctcg aatcaaactt atttaatgcg ggtatcagac
     3001 cagcaattaa cgtaggtatt tcggtttctc gtgttggcgg taatgctcag ataaaagcaa
     3061 tgaaaaaagt tgctggaagt ctgaagcttg atctagctca atacagagaa ttggaagcgt
     3121 ttgcaaaatt tggttctgat cttgataaat cgacacaaag aactttagct aaaggtgcaa
     3181 ggttagtaga attgcttaag caaggacaat acgctccggt acctgttgag aaacaagttg
     3241 ttagtattta ctatggtacc aaaggtttta tggatgatat tgcagttaaa gatgttaagc
     3301 gttttgaaaa agaagtgtta gaatttattg aggtcaaata caaacagatt tttgattcga
     3361 ttaaaaaaga taaagatgta agtaaagaga ccgaagaatt gattaaaaaa gccggaaaag
     3421 aatttttatc aacatttaaa tagcatagaa tattttcttt agataataat ggcaacatta
     3481 agagatataa aacttagaat taaaggtgta aaaagcactc agcagattac taaagcgatg
     3541 aagatggtcg ctgcggcaaa gctacgccgt gctactgaat caatactgaa tgcccgccct
     3601 tatgctaaaa aaatatctgt gttgctatca catcttgtaa atgaagatga taaaaattcc
     3661 aatcctttgt ttgctcaaag agaagttaag aatgttgcag ttgttgtagt tacagctgac
     3721 cgcggtcttt gcggagcttt taatactaac attattaaag aagcatcacg atatgttgaa
     3781 gaagatatca aacaaaaagg attagattat aaactgtttt gtttaggtaa aaaaggctca
     3841 gattatttta gcagaaggaa ttataacatt gccgctactt acccaggaat attttctctc
     3901 attgattata caacggcaca aaaattagtt gatgaaatta ttcccaaata tcttgatgga
     3961 agtattgata aagtaataat tg
//
LOCUS       DASWBY010000251         3182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_11351, whole genome shotgun sequence.
ACCESSION   DASWBY010000251 DASWBY010000000
VERSION     DASWBY010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11351"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VF870_04595"
     CDS             <1..856
                     /locus_tag="VF870_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9251494.1"
                     /translation="PYEFYMLANGLPYSISILLIITFHEFGHYFAARFHRIRATLPFY
                     IPFPPIPYFINFGTMGAVIRTKSPIYSKKAMFDIGIAGPVSGFIICLGILIYGFNHVP
                     SIEYLLQIHPDYFSPDYGKEGMQLVFGDSILFSFLRFIFVNPNTFFPPMSEIYHYPFL
                     CAGWFGLLVTSMNMIPVGQLDGGHIAYTMFGDKTHHNISAISTIILLVIGFAAVLDSL
                     LGFNLGFGWSGWLFWAFILYFVVKLKHPPVPDVLPLDRKRIFMGYISFFIFVISFSPA
                     PLMLNVVG"
     gene            911..1720
                     /locus_tag="VF870_04600"
     CDS             911..1720
                     /locus_tag="VF870_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251495.1"
                     /translation="MKIKILTILSLTFILTLFIGCEQKYDNTVENFIPDYQVKRVSPS
                     DSIRYNPADSLITIKIEFNSAANIQSVYCDVYAADNSKLNSSPLSLFDNGKSENGDNV
                     ANDGSFANKFPLSEFYPNGIYNIKYFVSDKSNSTNQVALGTFKYDNGQANISPVISDD
                     IVDPDTAVVTGTTIILTSINVFDQNGLSDIEKVYFLVYRPDGTTNNNQNLMFDDGNPV
                     LHGDQNAGDGIYSLLIQISSTNDKGTYRLEFQAKDRGGKLSNIINHNLLIQ"
     gene            1717..>3182
                     /locus_tag="VF870_04605"
     CDS             1717..>3182
                     /locus_tag="VF870_04605"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX9251496.1"
                     /translation="MTKYLILIINILLISVKVSAQLSPDNYILQEDGKTLSKTNSQNP
                     LSNTVTDIITVGDTIWLGTSRGVSLSIDGGETWTNFYGQADFGNDNVASIGYNNGVFW
                     CATARSTEVTGGSSLPEGTGLKYTTNLGATWTSIPQPLDNPDSTTEQYGNNIIQALPV
                     TVAIQNLAYDIAFTPNTIWIATFAGGLRKSTDNGQTWKRVILPPDNLNSIAPTDTLDF
                     CLSPVAGSFCGQGNLNHRVFSVISTDDNTLYVGTANGINKSTDGGISWIKFNAQNQSE
                     PISGNFITALGYNNVNHTVWASTWKAEKPEEFYGVSSSSDGGQTWRTFLRDERPHNFG
                     FKNFDVMVPTDNGVFRSSDNGLTWLLPNSIVDEASKTLLKTKLFYAADSYQNKIWLGS
                     ADGLVKLQETPGEMWEGSWKICFASKPLAANDETYCYPNPFNPRQEELKIKYSTGSTE
                     ANVTIRIFDFSFNYVRTVIQNVSRNRDLESSPEFWDGK"
BASE COUNT          996 a          525 c          576 g         1085 t
ORIGIN      
        1 gccgtatgaa ttttatatgc tggctaatgg attaccttac tcaattagta ttcttctgat
       61 aattactttt cacgagtttg ggcattactt tgcagcaaga tttcatcgta taagagcaac
      121 acttccattt tatattccat tccctccaat accgtatttc attaatttcg gcacgatggg
      181 agcagttatc agaacaaaat ctccgatcta ttcaaagaag gctatgtttg atattggaat
      241 tgcaggacca gtttcaggtt ttataatttg tcttggaatt ttgatttatg ggtttaatca
      301 tgttccatca attgagtatt tgcttcaaat ccatcccgat tatttttctc cggattatgg
      361 aaaagaaggt atgcaacttg tctttggtga ttcaattcta ttttcttttc ttagatttat
      421 ttttgtaaat ccaaatacat tttttccacc aatgagtgaa atttatcatt acccatttct
      481 ttgcgccggt tggtttggat tacttgtaac ctcaatgaat atgattcctg ttggacaatt
      541 agatggcgga catattgcat acacaatgtt tggagataaa acccatcata acatttcagc
      601 tatctctaca ataattttat tagtaattgg ctttgctgct gttttggatt ctcttttagg
      661 atttaatctg gggtttggat ggagcggctg gttgttttgg gcattcatat tgtactttgt
      721 tgttaagcta aaacatccac cggtgccgga tgttctgcct ttggatagaa aaagaatttt
      781 tatgggttat attagtttct ttatctttgt aatatcattt tcacctgcac cgctaatgct
      841 taatgtcgtc ggctgaaatg gttttatttt taccttgctg aagtttagtt attaagaaat
      901 atttagctat atgaaaatta aaatattaac aattctctct cttactttta ttctcactct
      961 ttttatagga tgtgaacaaa agtatgataa tacagtcgaa aattttattc ctgattacca
     1021 ggttaaacgt gtatctccat cagattctat tagatataat ccggctgatt ccttaattac
     1081 aattaaaatt gaatttaatt ctgctgcaaa tattcaaagt gtttattgtg atgtttatgc
     1141 agctgataat tcaaagctta attcttcacc attatcttta tttgataacg gtaaatcaga
     1201 aaatggtgat aatgtagcaa atgatggatc ttttgcaaat aaatttccgt taagtgaatt
     1261 ttatccaaat ggaatttata acataaaata ttttgtaagt gataagtcaa actcaactaa
     1321 tcaggttgcc ttgggtacct ttaagtatga taatggtcaa gctaatatct cacctgttat
     1381 ttctgatgat attgttgatc cggatactgc tgttgtaacg ggaactacaa ttattctaac
     1441 ttcaataaat gtatttgatc aaaacggatt atctgatatt gaaaaagttt attttctggt
     1501 ttaccgcccg gatggaacaa ctaataataa tcaaaacctg atgttcgatg acggcaatcc
     1561 ggttttacat ggtgatcaaa atgctggtga tggaatttat tcacttttaa ttcagataag
     1621 ctcaacaaat gataaaggta cttacagatt agaatttcag gcaaaggatc gcggtggtaa
     1681 actaagtaac atcataaatc ataatttact tatacaatga cgaaatattt aattcttatt
     1741 atcaacatcc ttcttatttc agttaaagtt tctgcgcagc tttctcctga taattatatt
     1801 cttcaggagg atggcaaaac attaagtaaa acaaattccc agaatccttt aagcaataca
     1861 gtcaccgata taattactgt cggtgataca atttggcttg gcacaagccg cggtgttagt
     1921 ttatcaattg atggcggaga aacctggact aacttttacg ggcaggccga ttttggaaat
     1981 gataatgttg catcaatagg ttataacaat ggagttttct ggtgcgctac tgcgcgatct
     2041 acagaagtaa ccggcggaag ttcattacca gagggaaccg gtttaaagta cactacaaat
     2101 cttggagcaa cctggacaag cattcctcaa ccgctggata atccagacag tacaacagaa
     2161 caatatggaa ataatataat acaagctttg ccagtcacag tagccattca aaatcttgct
     2221 tatgatattg catttactcc aaatacaatt tggattgcca cttttgcagg cggactaaga
     2281 aaaagtacag ataacggaca aacctggaag agagtaatac ttcctccgga taatctaaac
     2341 tcaatcgcac caactgatac tttagacttt tgtctttcac cagttgcagg aagtttttgt
     2401 ggacagggaa atttaaatca tagagttttt tcggtaatct caacagatga taatacttta
     2461 tatgttggta cggctaacgg aattaataaa tctactgatg gtggaatcag ctggataaaa
     2521 tttaatgctc agaatcagtc cgaacctatt agcggtaatt ttataacagc actcggatat
     2581 aataatgtta atcatacagt ctgggcatct acctggaaag ctgaaaaacc tgaagagttt
     2641 tacggtgtta gttcatcctc tgatggcgga cagacatgga gaaccttttt aagagatgaa
     2701 cgcccgcata attttggatt taaaaacttt gatgtaatgg ttccaactga taatggagtt
     2761 tttcgatctt cagataatgg acttacctgg ctgcttccaa attcaattgt tgatgaagct
     2821 tccaaaactt tattaaaaac aaaattattt tatgctgctg attcatatca aaataaaatt
     2881 tggttaggtt cggccgatgg tttggttaaa ttgcaggaga cacctggtga aatgtgggaa
     2941 ggaagctgga agatttgctt tgcttcaaaa ccacttgcag caaatgatga aacttattgt
     3001 tatccaaatc cattcaatcc ccgacaggaa gaattaaaaa taaagtacag cactggcagt
     3061 actgaagcta atgttacaat ccgcattttt gattttagtt ttaattatgt aagaacagta
     3121 attcaaaacg tgtcgagaaa tcgtgatctt gaaagttcac ctgagttttg ggacggaaaa
     3181 ga
//
LOCUS       DASWBY010000252         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_160655, whole genome shotgun sequence.
ACCESSION   DASWBY010000252 DASWBY010000000
VERSION     DASWBY010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160655"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VF870_04610"
     CDS             <1..1378
                     /locus_tag="VF870_04610"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9251497.1"
                     /translation="AVGVNYYYNEDTQKIEVKFDKYSVKASARNPFLVLNEKSSNNQL
                     IIQLPTSTYLINKKVYVPLIYSLNTLGIALGKELSFEAPNKIIIGKVKEEITKTADET
                     SNKFNITGIILNEKANGTLITVKSNKRIPSYLSAFKNNVLTLTFRKVNVDIDKVKFIG
                     EEGVVKKIEAKNVDADAILNITVSKEYTTNEVMNIDNSNDIQITIHNKLFNQTNSSNK
                     LKDKWEFDVIVIDAGHGGQDAGAIGVNGVKEKDINLAIALKLGKLIEENMKDVKVVYT
                     RKTDVFIDLYKRGKIANEKGGKLFISIHCNSTPKKPSDANGFEVYLLRPGRTKEAISI
                     AEFENSVIKYEENPNRYEKLTDENFILVSMAHSSYMKYSERFAEYLHKEFSNHSTLSS
                     RGVKQAGFYVLVGASMPSVLIESGFLSNTNDAKHLSTSKGQQKFAEYVFNGIKKYRES
                     YELEMQNE"
     gene            1551..1988
                     /gene="tnpA"
                     /locus_tag="VF870_04615"
     CDS             1551..1988
                     /gene="tnpA"
                     /locus_tag="VF870_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX9251498.1"
                     /translation="MSWIRIWVHLVFSTKNREPFLNSPELRKSVFRHIKQNAEAKDIW
                     IDQICGYADHIHCLVSLGREQSISKVAQLIKGESSHWINENNLTKSKFIWQDDYWAVG
                     VSESHLDSIRQYIQDQENHHKKKSFSDEIELFMKKYGWKYLKE"
     gene            2179..>2494
                     /locus_tag="VF870_04620"
     CDS             2179..>2494
                     /locus_tag="VF870_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251499.1"
                     /translation="MKFILSQFLVLVFLLLSLSCNTEVTYPDSALGLALKDFSCTEAW
                     IEVQIGANDKTANIYIKKNDEIIQTVNVGESETLFYFDDLLPNTTYRFDAVTYDDGTE
                     IKS"
BASE COUNT          910 a          350 c          458 g          776 t
ORIGIN      
        1 tgcagtggga gttaactatt actacaacga agacacacaa aagatagaag ttaagtttga
       61 taaatattct gttaaagctt ctgcaagaaa cccgtttctg gttctgaatg aaaaaagcag
      121 taacaaccaa ttaattattc aacttccaac ctcaacttat cttataaata aaaaagttta
      181 tgtcccgctt atttatagtt taaatacact tggaatagct ttgggtaaag aactaagctt
      241 cgaggctccg aataaaatta ttattggtaa ggttaaagaa gaaatcacca aaacggcaga
      301 tgaaacatca aataaattca atataaccgg tattatactg aatgaaaaag caaatgggac
      361 cttaattaca gttaaatcca acaaaagaat tccatcctat ttaagtgcgt ttaagaacaa
      421 tgttctaact ttaactttta gaaaggtaaa tgttgatatt gataaagtta aatttatcgg
      481 ggaagagggc gttgtaaaaa aaattgaagc taaaaatgtt gatgctgatg caatacttaa
      541 cattactgtt agtaaagaat ataccacgaa tgaagtgatg aacattgata acagtaatga
      601 tattcaaatt acgattcata ataaattatt taatcaaact aattcatcaa ataagcttaa
      661 agataaatgg gagtttgatg taattgttat tgatgcagga catggcgggc aggatgctgg
      721 tgctattggt gtaaacggag ttaaagaaaa agacatcaat cttgcaatag cgttaaagct
      781 tggaaaactg attgaagaaa atatgaaaga tgtaaaagtt gtatatacac gtaaaacaga
      841 tgtatttata gatctttaca aaaggggtaa gattgccaat gaaaaggggg gtaagctttt
      901 tatctcaatt cattgcaact caacaccaaa gaaaccaagc gatgcaaatg gatttgaagt
      961 ttacttgctc cgtccgggca gaacaaaaga agctatttct atagccgagt ttgaaaacag
     1021 tgtaattaag tacgaagaaa atcccaatcg ttatgaaaaa ctgaccgatg aaaattttat
     1081 tctggttagt atggcccatt cctcttatat gaaatactct gaacgctttg cagaatatct
     1141 ccataaagaa tttagcaatc attctacact ttcttcacgc ggagttaagc aggcaggatt
     1201 ttatgtcctc gtcggtgcat caatgccaag tgttctgatc gaaagcggat tcctttccaa
     1261 tacaaacgat gcaaaacatt taagcacttc taaaggtcag caaaaatttg cagagtatgt
     1321 ttttaatgga ataaaaaaat accgcgaaag ttatgaactt gaaatgcaga atgagtaatg
     1381 aaaatatcaa tttggctaaa ttcaaaaatt ttttaatttc ttttacagtt gacgactgtc
     1441 ataaataatt attataatta aaatttagga taatctattg cagtcagctt tagctgactg
     1501 aacaatgatt taatcttaat tgtggcttta gccgcattgg aggaattagt atgagttgga
     1561 taagaatttg ggttcatctt gttttctcaa ctaagaatag agagccattt ctaaatagtc
     1621 cggagttgcg taagagtgtt ttcagacata taaagcaaaa tgcagaagca aaagatatat
     1681 ggattgatca aatttgtggg tacgctgatc atattcattg tttagtatca ttaggcagag
     1741 aacaaagcat aagcaaagtt gctcagctga ttaaaggaga atcttctcat tggataaatg
     1801 aaaataactt aacaaaaagt aaatttatct ggcaggatga ttattgggct gttggagtta
     1861 gcgaaagtca cctggattct ataagacaat acattcaaga tcaggaaaat catcacaaga
     1921 aaaagtcttt tagtgatgag attgaattgt ttatgaaaaa atatggctgg aaatatttaa
     1981 aggaatgatg tggctaaagc caaagttttt ttaattgctt ttgcagttga cttaagtcaa
     2041 ctgcaataaa tttgattttc taacacctat tgcagtcagc tttagctgac tgaataatta
     2101 attaaaagta atatggcttt agcctcattt agtcttttat taaacttgca ttgctaattt
     2161 ctataatagt gggtttttat gaagtttatt ctatctcaat ttttggtatt agtgtttttg
     2221 cttctttcat tatcctgtaa tacggaagta acatatccgg attctgcatt aggcctagcg
     2281 cttaaagatt tttcctgcac agaagcctgg attgaagtgc agattggagc aaatgataaa
     2341 acagcaaata tctacatcaa aaaaaatgat gagattatac agactgtaaa tgttggtgag
     2401 tctgaaactc tattttattt tgatgactta ttgccaaaca caacctacag gtttgatgca
     2461 gttacttacg atgacggtac agaaataaag agta
//
LOCUS       DASWBY010000253         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_723337, whole genome shotgun sequence.
ACCESSION   DASWBY010000253 DASWBY010000000
VERSION     DASWBY010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723337"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1620)
                     /locus_tag="VF870_04625"
     CDS             complement(<1..>1620)
                     /locus_tag="VF870_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEX9251500.1"
                     /translation="YQAEIAQGRLEALLNFQTAITDLTALPIANASLLDEATSAAEAM
                     IMFFNNRKKDKANSKKFFVSNEVLPQTIDVLKTRSKPLGIELVIAEHITIELTNDYFG
                     LLVQYPALNGEIYDYTELFSKAHALGIYKVVAADILSLTLLTPPGEFGADCAVGSTQR
                     FGIPMGFGGPHAAYFATKEDFKRVIPGRIIGVSVDSQGNRALRMALQTREQHIKRERA
                     TSNICTSQVLLAVIAGMYTVYHGPKNLKLIAERINNLTKVLGKGITELGLIQVNKNYF
                     DTISFKVDKNLNEKINSEALKHKINLNYFIKDHISISLSEVTTLPDVKELIQVFALAV
                     GKQPDNLNDLDIQINLISSFDEKFVRTSKYLEHPVFNSYRSETDILRYMKYLENKDLS
                     LVHSMIALGSCTMKLNGTTEMLGVTYPEFGNIHPFAPKDQVGGYLDLFRELEKDLAEI
                     TGFAGVSLQPNSGAQGEYAGLSVIKAYLEDKGDTHRNIALIPSSAHGTNPASAVMAGM
                     KVVVVNCDNHGNIDLNDLQAKAELHKNNLAAL"
BASE COUNT          508 a          306 c          255 g          551 t
ORIGIN      
        1 taaagctgca agattatttt tatgcagttc agcttttgcc tggagatcat ttaaatcaat
       61 atttccatga ttatcacagt ttacaactac tactttcatt ccggccatta ctgcacttgc
      121 aggatttgtt ccatgagctg aagaaggaat taaagcaatg tttctatgcg tatcaccttt
      181 atcttctaaa taagctttga taactgataa accggcgtac tcaccttgtg cgcctgaatt
      241 tggctgaagc gataccccgg cgaagcccgt tatttctgcg agatcttttt ctaattctct
      301 aaataaatct aaatatccgc caacctgatc tttaggtgca aatggatgaa tatttccaaa
      361 ttctggataa gtaactccca gcatttcagt tgttccattt agtttcattg tgcacgatcc
      421 aagtgcgatc attgagtgca caagagataa atctttgttc tcaagatatt tcatatatct
      481 taaaatatca gtctctgatc tataagaatt aaaaacggga tgctctaaat attttgatgt
      541 acgaacaaat ttttcatcaa acgatgaaat taaattaatt tgaatatcga gatcattcaa
      601 attatcaggt tgtttcccaa ctgccagagc aaaaacttga atcaattcct tcacatctgg
      661 gagtgttgta acttcactta aagaaatgct tatatgatct ttaataaagt aatttagatt
      721 tatcttatgt tttaaagctt cagaattaat cttttcattt aaatttttgt ctaccttaaa
      781 tgaaatagta tcaaaataat ttttgttaac ctgaattaag cctaattcag taattccttt
      841 acccaaaact ttcgtaagat tattaattcg ctcagcaata agtttaagat ttttaggacc
      901 atgataaaca gtgtacattc cggctataac agctagcagt acctgagatg tacaaatatt
      961 acttgtcgct ctttcacgtt taatatgctg ctctcttgtt tgaagagcca ttcttaaagc
     1021 tctgtttccc tgtgaatcga cagaaacacc aataattcta ccgggaatta ctcttttaaa
     1081 atcttctttg gttgcaaaat aagccgcgtg cggaccgccg aatcccatcg gtatcccaaa
     1141 tctctgagta ctgccaacgg cacaatcagc accaaattca cccggaggtg taagaagagt
     1201 aagactcaaa atatcagctg caacaacttt ataaattcct aatgcgtgcg cttttgaaaa
     1261 caattcagtg taatcataaa tttcgccatt aagtgcagga tactgtacta ataaaccaaa
     1321 ataatcgtta gttaattcta ttgtaatatg ttcagcaata actaattcga ttcccaatgg
     1381 tttagatctt gttttaagca catcgatcgt ctgcggaagt acttcattag aaacaaaaaa
     1441 tttctttgaa ttagctttat ctttttttct gttattgaag aacataatca tagcttcagc
     1501 agcggatgtt gcttcatcaa gcaaagatgc atttgctatt ggtagtgcgg taagatctgt
     1561 tatggctgtt tgaaaattaa gcaatgcttc caatcttccc tgagcaattt ctgcttgata
//
LOCUS       DASWBY010000254         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_196291, whole genome shotgun sequence.
ACCESSION   DASWBY010000254 DASWBY010000000
VERSION     DASWBY010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196291"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VF870_04630"
     CDS             <1..653
                     /locus_tag="VF870_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEX9251501.1"
                     /translation="YFILVADSSALFYYNLFNYNNKNIIGSSSLGLVNTGELILLKDV
                     RGNVIDSVYYDSKWNNRNIASTKNKSLERINPSLNGNDPLNWSTSVNSLGGTPGMQNS
                     IFAENLNQSANISVYPNPFSPDNDGFEDFTVINYNLSQATAQVRIKIFDSKGRLVRSL
                     LNNQASGSSGSVIFDGLDDENNALRMGIYIILLEALNDNSGIVETIKSTVVVARKL"
     gene            759..2006
                     /locus_tag="VF870_04635"
     CDS             759..2006
                     /locus_tag="VF870_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9251502.1"
                     /translation="MRLTTKTILVLSLFISFISHSQTEVNTVNGEIVEFDKCGFPMDD
                     SDLGQFISPEYMGYSYDSLLIDLELWRTSKYVKIDSIGLSVQGRPLWQLTVSSDPDNV
                     AGKRTVHIHARTHPQETEGFYVTEEILNILLSESELAQTARANCVFYIIPMYNPDGVE
                     LGLPRRNANNVDLEANWNTFPHQPEVAALKARFIQLMASPNPIEVMLNMHSSSLCTRY
                     FVYHHENGTSPTYAVLQQNFINGVRSYYPNAIEPYSYFVSWASGTALQYPESWCWLNH
                     GENILAITYEDKYKPSCPTSGNYDSTANAILRGVLDYMNITTDVEQIGNLAPAEFRLE
                     QNYPNPFNPCTSIQYAISKRQFVNLKVYNLLGNEIATLVDEYKPAGIHNVQFTMYNLA
                     SGIYFYQLKTENLVQTKKMILLK"
     gene            2174..3880
                     /gene="recN"
                     /locus_tag="VF870_04640"
     CDS             2174..3880
                     /gene="recN"
                     /locus_tag="VF870_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX9251503.1"
                     /translation="MIKTLEIKDYALIDHIKVEFEKGLNIITGETGAGKSILIDAMSL
                     LLGERASTEVVRKGTQKSFVEGIFDVELNKKVKSFLEENEIEFQPELIIRREISLKGS
                     NRCFVNDSPVPLNLIKELGDLLVDLHGQHEHQSLLRTETHIEFLDEFAGNQKLLEDYK
                     NLYKGLNSKQNELKDLNSKEELLKEKKEIYGFQIKEIDSINPLRDEDELISNELNILE
                     NSEKLLELTDDVYNKLYDGEPSVIDLLGETKHKLNQLANIDKSFLESEGECDSALAVV
                     KELANSLRNYKSRIDVDPKEIEEKRERLGSLNLLKKKYGGSIQKIFEHREKIGKEFEL
                     ADNFSNAISNLEKEIFGLRKSAGEAAEKISVSRKKHAVKVESEVRKVLAQLGIPDAVF
                     KVNITHNEADKDENHSVAAKSRNYSCTETGIDEVEFYISTNAGEDAKPLTKVASGGEI
                     SRIMLSLKTILAKSDKLPLLIFDEIDTGISGRIAQKVGIALKDLTSFHQIIAITHLPQ
                     IAGMANNHFAVEKKQIDNRAVSSIKKLSDDERINEIAKLISGEDLSKASIESAKQLIF
                     NK"
BASE COUNT         1417 a          600 c          730 g         1200 t
ORIGIN      
        1 agtatttcat tttggttgct gattcttcgg cattgtttta ttacaatctt tttaattata
       61 acaacaaaaa tataatcgga tcatcaagtc ttggacttgt aaacactgga gaattaattt
      121 tattaaaaga tgtaagggga aatgtaattg attctgttta ctacgacagc aaatggaata
      181 atagaaacat tgcttcaaca aagaacaaat ctttagaaag aattaatcca agcttaaatg
      241 gtaatgatcc tttaaactgg agcacatctg tcaattcgct tggcggaaca ccgggaatgc
      301 aaaatagtat ctttgcagaa aatttaaatc agtccgctaa tatttcagtt tatccaaatc
      361 cattttcacc tgataatgat ggctttgaag attttacagt tataaattat aatttatcac
      421 aagccactgc acaggtacga ataaaaatat ttgacagtaa gggcagatta gttcgttcat
      481 tattgaacaa tcaggcaagc ggttctagcg gctctgtaat ctttgatggg cttgatgatg
      541 aaaacaatgc tttgcggatg ggaatatata tcattttatt agaggcactt aatgataact
      601 caggtattgt tgaaaccata aaatccacag ttgttgttgc ccgcaaactt taagattaga
      661 gtgagttttt ttttgctgct ttaattattc tgttgataaa cgctgcaggt cacaaatcaa
      721 attcgtattt ttagatcaat tatatttaat ggattaaaat gcgcttaaca acaaaaacta
      781 tcctggttct ttcacttttt atatcattca tttcacactc acaaactgaa gtaaacacgg
      841 taaatggtga aattgttgaa tttgataaat gtggttttcc aatggatgat tcagatttgg
      901 gacaattcat ttcccctgaa tatatgggtt atagttatga tagtttatta attgatcttg
      961 aactatggcg aacaagtaaa tatgtgaaga tagattcaat agggttaagt gtgcaaggca
     1021 gaccattatg gcaattaaca gtttcaagtg atccggataa tgttgccgga aagcggactg
     1081 ttcatattca tgcaagaaca catccgcagg aaacagaagg attttatgtt accgaagaaa
     1141 tcctgaatat tcttttatct gaaagtgagt tagcacaaac ggcaagagcg aactgcgtgt
     1201 tttatataat accgatgtac aatcctgatg gtgttgaatt agggttgcca aggagaaacg
     1261 caaacaatgt tgatcttgaa gctaattgga atacgtttcc acatcagcct gaagttgctg
     1321 ctctaaaagc aaggtttata caactaatgg cctcaccaaa tcccattgaa gtaatgttaa
     1381 acatgcattc gtcttctcta tgcacaagat attttgttta tcatcatgaa aatggaactt
     1441 caccaacgta tgcagtatta cagcagaatt ttattaatgg tgtgcgatct tattatccaa
     1501 acgcaattga accttatagt tattttgtta gctgggcctc cggaacggca ctgcaatatc
     1561 ccgaaagctg gtgctggtta aatcatggag aaaacattct tgccattacg tatgaagata
     1621 aatataaacc atcttgccca acttccggga attatgattc aacagcgaat gcaatattgc
     1681 gtggagtttt agattatatg aatatcacca ctgatgttga acagattgga aatttagcac
     1741 cagctgaatt tagactagag caaaactacc cgaatccatt taatccatgc acgtctattc
     1801 agtatgcaat aagcaaaagg caatttgtaa accttaaagt atataattta cttggaaatg
     1861 aaattgcaac actggttgat gaatacaaac cagcaggtat tcacaatgta caattcacaa
     1921 tgtacaattt agcttcggga atttatttct atcagttaaa aaccgaaaat ttagttcaga
     1981 caaaaaagat gatactttta aaataacctt ggcggctttg cgtctctgcg tgaaatgaat
     2041 tataaaactt cacgcagaac tctcaaagat ttctgctaag aatgcaaaga attttagcgt
     2101 aaattttcta aacctcactc tcgctcttaa tcttaatctt ctttattttt ccgccaataa
     2161 attcaatatc aaaatgatta aaacattaga aataaaagat tacgctctta tcgaccacat
     2221 taaagttgag tttgagaagg gacttaatat tattaccgga gaaacaggcg caggtaaatc
     2281 tattttgatc gatgcgatga gtctgctcct tggtgaacgt gcttcaactg aggtagtacg
     2341 gaaaggcaca caaaaatctt ttgttgaggg aatatttgat gttgaactga ataaaaaagt
     2401 aaaatcattt cttgaagaaa atgaaattga atttcagccc gaactaatta tccgtagaga
     2461 aatatcgtta aaaggatcga atagatgttt tgttaatgac tccccggttc cattaaatct
     2521 gattaaagag cttggtgatt tacttgttga tttgcatggc cagcatgaac atcaatcatt
     2581 gctgcggaca gaaactcaca ttgagttttt ggatgagttt gccggtaatc aaaaattact
     2641 cgaggattat aaaaatctat ataaaggatt aaattctaag cagaatgaat taaaagattt
     2701 gaattcaaaa gaagaattat taaaagagaa gaaagagatt tacggatttc agattaaaga
     2761 aattgatagc attaatccat tgcgagatga agatgaatta atcagtaatg agttaaacat
     2821 tcttgaaaac tctgaaaaac ttttagagtt aaccgatgat gtttataaca agctttatga
     2881 cggtgagcca tcagttattg atttgcttgg cgaaacaaaa cataaattaa atcaacttgc
     2941 aaatattgat aaatcctttt tagaatctga aggtgagtgt gattctgctt tagccgttgt
     3001 aaaagaactt gcaaactcgc tgcgtaatta taaatccagg attgatgtcg acccaaaaga
     3061 aattgaagaa aaacgtgaga gacttggatc actaaatctt cttaagaaaa aatacggcgg
     3121 atcgattcaa aagatatttg aacacagaga aaaaatcggg aaagaatttg agcttgctga
     3181 taatttttct aatgcgataa gtaatctgga aaaagaaata ttcggattaa gaaaatcggc
     3241 aggtgaagca gcagaaaaga tttctgtatc aagaaaaaaa cacgctgtta aagtagagag
     3301 tgaagtaagg aaagtgttag ctcaactcgg aattcctgat gcagtattta aggtgaatat
     3361 cactcataat gaagctgata aagatgagaa tcattcggtt gcagcaaaaa gtagaaatta
     3421 tagctgtaca gaaacaggaa ttgatgaagt tgaattttat attagcacaa atgcaggaga
     3481 agatgcaaaa ccgttaacaa aggttgcttc gggtggagaa atatcacgca taatgctttc
     3541 actaaaaact attcttgcaa aatcggataa gcttccttta ttaatctttg atgaaattga
     3601 taccggtatc agcggaagaa ttgcacaaaa agttggcatc gctttaaaag atctgacttc
     3661 attccaccaa ataattgcta ttacacattt acctcaaata gccggaatgg caaataatca
     3721 ttttgcagtt gagaaaaagc aaattgacaa tcgagccgtc agttctataa aaaaactttc
     3781 tgatgatgaa cgtattaatg agattgcaaa actaataagc ggtgaagatt taagtaaagc
     3841 cagcatagaa agtgctaagc agcttatctt taataaataa aaatagtttt tgtatctaaa
     3901 aaaagattat aggaactttg tattagaaat tctgaatgaa aggaaat
//
LOCUS       DASWBY010000255         4797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_160744, whole genome shotgun sequence.
ACCESSION   DASWBY010000255 DASWBY010000000
VERSION     DASWBY010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160744"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF870_04645"
     CDS             complement(<1..279)
                     /locus_tag="VF870_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9251504.1"
                     /translation="MKDKVIIITGANKGIGKETAKEIAKLGAKVYMACRSLDSANEAR
                     EEIIKETKNENVFVKKLDLASLDSIKEFATSFKNEEAKLDVLINNAGIA"
     gene            complement(443..997)
                     /locus_tag="VF870_04650"
     CDS             complement(443..997)
                     /locus_tag="VF870_04650"
                     /inference="COORDINATES: protein motif:HMM:NF020136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HEX9251505.1"
                     /translation="MNFLTLTLSLIIMISSSLQNQDSLLLNASLMKTENKWRVVNDGV
                     MGGLSSSKAFVEDDKIIFSGNVSLENNGGFASLRSPVKDYNFEKYNGIEIKIKGDGKQ
                     YSISMKETYYFTGYFYTTSFDTKKDEWMVIKISFDKFKLYYYGKETNSKKKIPLSNIK
                     EVSLMIGDKQEGSFKAEIEYLGLY"
     gene            complement(1089..1970)
                     /locus_tag="VF870_04655"
     CDS             complement(1089..1970)
                     /locus_tag="VF870_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251506.1"
                     /translation="MKRFIQQLFLLLIFILLGNRLISAQSTDTTQATPKGKDRILPFY
                     SDWAKEQGIDLPLPFGISANYIFMSRDVDVTDVTVQFLNRVPESINDFTSFAVRNKTS
                     VAAVKFDAWVLPLLNLYVSIGYASTNANMNANIIIERSIAPLPPVEIDIETKTKIADP
                     YWGVGATLVAGYKSWFIMADANYGATSPDELNNSIDFTFLSARTGFAGNLGQENSIKA
                     WLGAAYLFSTTTLEITVPTETLGDVLVRIEQEPVDPWTFHSGFMISLNKRFDFMAELG
                     TNFQDASIAVLSTTFRF"
     gene            complement(2184..2990)
                     /locus_tag="VF870_04660"
     CDS             complement(2184..2990)
                     /locus_tag="VF870_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX9251507.1"
                     /translation="MSREKANQFVERKSFQNFIISLIILNAITIGMETSSEIMILFGR
                     TLLLIDKIILIIFVIEILLKLYAYGFGFFKSGWNIFDFSIVAIALLPASGPLAILRAL
                     RIFRSLRLIKNVPKLRFIVESLFHSLPSLVWIFVLLSLVFYVFSVIGTKLFGADYPQW
                     FGNLGASMFSLFQIMTLEGWAEISRSIMEKYPLANIYFILFILLASYTTLNIFIAIVV
                     NTMAEVQQKVSEDEISKIEEFIQDENEELKQNIRLIKEQVIKIEERLMKK"
     gene            3154..>4797
                     /locus_tag="VF870_04665"
     CDS             3154..>4797
                     /locus_tag="VF870_04665"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX9251508.1"
                     /translation="MSFKIIWTKIDEAPALASYCLLPILKSFTKGTIIEFEERDISLA
                     GRIIANFPENLTEEQKIPDYLAELGELTQDPDANIVKLPNISASIPQLKAAIKELQEK
                     GYKIPDYPEEPKTAEEKKLKERFAKVLGSAVNPVLREGNSDRRAAASVKKFAQKHPHK
                     MMKPWPESGSKTRVAHMSQKDFYGSETSMTMDKEDIVKIEFVDENGKSSVLKENLKLL
                     KDEVIDTAVMNVKELRKFYAEQIEEAKKDNVLLSLHLKATMMKISDPIMFGHAVSVYY
                     KDALEKHAQTLKEIGANVNNGLMDILEKLKKLPDEKRKEIENDINKVYEYQPELAMID
                     SRIGKTNLHVPNDVIVDASMPNIVRDGGKMWNKKDELQDCIAMIPDRSYATIYQTIIE
                     DVKTNGQFNPATMGAVSNVGLMAKKAEEYGSHDKTFEATGNGVIRVINSEGKTLLEQR
                     VETGDIFRMCQAKDEAIKDWIKLAVKRAKASSTPAVFWLDENRAHAKELIVKIKKYLT
                     ELDTTGLEIKILKPVDAMKYTLERTRKGLDTISVTGNVLR"
BASE COUNT         1642 a          822 c          811 g         1522 t
ORIGIN      
        1 agcaattccg gcattgttga ttagcacatc aagcttagct tcttcattct taaaagaagt
       61 cgcaaattct tttattgaat caagcgaagc aagatccagt tttttaacaa atacattttc
      121 attcttagtc tcttttataa tttcttctct tgcttcattt gcagaatcaa gagacctgca
      181 agccatataa acttttgcac caagctttgc aatttccttt gcggtttctt tcccgattcc
      241 cttattagcc ccggttatta ttattacttt atctttcatt ttttcctctt gctcttgtgt
      301 tgtatgaatt gaaacaaaag acagagtaga atcattcctt tctgtctttt aatagcttca
      361 ttaagctgac caaaagatta ttagggttag caaaatcagg tttgttaatt cccattagat
      421 ttgtttttag ttattgatcg tttcagtata gccctagata ttcaatttct gctttaaaac
      481 tgccttcttg tttatctcca atcatcaaag atacttcctt gatattgctt aggggaattt
      541 tcttttttga attggtttct ttcccataat agtaaagttt aaacttatca aatgaaattt
      601 taattaccat ccactcatct tttttcgtat caaatgatgt tgtataaaaa taaccagtaa
      661 agtaatatgt ttccttcatt gaaatgctgt attgctttcc atcacctttt atctttattt
      721 ctattccatt atacttttca aaattgtaat ctttaaccgg acttctaagc gacgcaaatc
      781 cgccgttatt ttctaacgac acattcccgc tgaaaattat tttatcatct tcaacaaaag
      841 cttttgaaga acttaaaccg cccatcacgc catcatttac aacacgccat ttgttctcgg
      901 ttttcattaa tgaagcattc agtaacaagg aatcctggtt ttgtaaagaa gaagagatca
      961 taataattaa tgataatgtg agtgttaaaa aattcatttt attgctttaa gttaatagta
     1021 tgttaaaaac tgtaaaacaa tatttaattt ttattcgttc actctgattt taaaaagaac
     1081 aaatcgattc aaaaacgaaa cgttgtactt aaaaccgcaa tagaagcatc ttgaaagttt
     1141 gttcctaact cagccataaa atcaaatcgc ttgttcaggc tgatcataaa tccggagtga
     1201 aatgtccacg ggtctaccgg ttcttgttcg attcgtacaa gcacatcgcc taatgtttcc
     1261 gtgggtactg taatttcgag agttgttgta gagaaaagat acgctgcacc gagccaggct
     1321 ttaatagaat tttcttgtcc caaatttccg gcaaaacccg tgcgtgcaga aagaaaagta
     1381 aaatcaattg agttgtttaa ttcatccggc gatgttgctc cataatttgc atctgccata
     1441 ataaaccagc ttttatatcc ggcaactaaa gttgcaccaa ctccccaata cggatcggct
     1501 attttagttt tagtttcaat gtctatttca accggcggaa gaggtgcaat tgatctttca
     1561 attataatat tcgcattcat atttgcattt gtgcttgcat atccaattga cacatataaa
     1621 tttaataatg gcagcaccca ggcatcaaac tttactgctg ctacagaggt tttatttctt
     1681 acagcaaagc ttgtaaaatc attaatgctt tccggaaccc tattaagaaa ctgaacggta
     1741 acgtctgtta cgtccacatc ccgactcata aaaatataat ttgcagaaat accaaaaggt
     1801 aacggtaaat caattccctg ttcctttgcc caatcagagt agaagggaag aattctatct
     1861 tttccttttg gggttgcttg tgttgtatca gttgattggg cagatattaa acgattacct
     1921 aataggataa aaattaaaag aagaaaaagt tgttgtataa atcttttcat aaagcaaaaa
     1981 aatttttgtg gaggatacaa aatctttaag ataaacttaa ttatttgaac ggcgagttgc
     2041 taatttttgc ggcagaaatt cgtagctgat tccttaatcg tatagttttt agttttgttt
     2101 tcactggttt tatgatttgt tataatttta cttctggcgt tggcacccac tttttgtaaa
     2161 ctgtaattaa aaaaatgaga ttttcatttt ttcatcagtc tttcctcgat ttttattacc
     2221 tgctctttga tcagacggat gttctgtttt aactcttcat tctcatcttg aatgaattcc
     2281 tcaattttgc ttatttcatc ttcacttact ttttgctgca cttctgccat agtatttact
     2341 acgattgcga taaagatgtt cagagttgta tatgaagcaa gcaggataaa tagaataaaa
     2401 taaatatttg caagcggata tttttccatt attgatctcg aaatttctgc ccatccttcc
     2461 agtgtcatta tttgaaacaa tgaaaacatt gaggcaccaa gattaccaaa ccactgagga
     2521 tagtctgccc caaagagctt ggtaccaatt actgagaaga cgtaaaacac gagtgaaagc
     2581 aaaacaaaaa tccacacaag acttggaagt gaatgaaaaa gtgattcaac tataaatcta
     2641 agtttaggta cgtttttaat cagtcttaat gaacggaata ttcttaatgc tcttaaaata
     2701 gccagcggac cggaagcagg aagcaatgca attgcgacaa ttgaaaaatc aaaaatattc
     2761 caaccgcttt taaaaaaccc aaagccataa gcatatagtt ttaataatat ttcaattaca
     2821 aaaattataa gtattatttt atctataagt aaaagcgttc ttccaaataa gatcattatt
     2881 tcactggaag tttccatgcc gatagtaatg gcattaagaa taattaaaga tataatgaaa
     2941 ttttggaaag atttcctttc tacaaactgg tttgcttttt ctctgctcat aaaatttaaa
     3001 ttgttaatgg tgaaggcaaa attaaaatat tcatgctgtt caaacaatca gaataatatg
     3061 aatatcatga gttttccttc aattattact accgtttgag tatttttaat taatagttta
     3121 aaaaattttc taaataaaaa acggatataa caaatgagct ttaaaattat atggaccaaa
     3181 atagacgaag cccccgcact tgcaagttac tgtttactgc cgatcttaaa atcttttaca
     3241 aaaggaacca ttattgagtt tgaagaaaga gacatatcgc ttgcaggaag aataattgcg
     3301 aactttcctg aaaatctgac tgaggaacaa aaaattcccg actacttagc agaacttggc
     3361 gaactgactc aagaccccga tgccaatatt gttaagcttc ctaatatcag tgcatcaatt
     3421 ccgcagctaa aggcagcaat aaaggaatta caggaaaaag gatataaaat tcctgattat
     3481 ccggaggagc cgaaaacagc tgaagagaaa aaattaaaag aaaggtttgc aaaggtactc
     3541 ggctctgctg ttaatcctgt gctgagagaa ggcaattctg atagacgtgc ggcagcttct
     3601 gttaaaaagt ttgctcaaaa acatccacat aaaatgatga aaccatggcc tgaatccgga
     3661 tctaaaaccc gggttgcaca tatgagccaa aaagattttt atggttctga aacatctatg
     3721 actatggata aagaagatat tgttaaaatt gaattcgttg atgaaaatgg aaaatcatca
     3781 gtgttaaaag aaaatcttaa gcttcttaaa gatgaagtga ttgatactgc agtaatgaat
     3841 gttaaggaac taagaaaatt ttatgcagag caaattgaag aagcaaaaaa agataatgtg
     3901 cttctttcac tgcaccttaa agctacaatg atgaaaattt ctgacccgat aatgttcggt
     3961 cacgcagttt ctgtttacta taaagatgca cttgaaaaac acgcgcaaac tcttaaagaa
     4021 ataggtgcaa acgtaaataa tggtttaatg gacatacttg aaaagttaaa aaaactccct
     4081 gatgaaaaaa ggaaagaaat agaaaacgat attaataaag tttatgagta tcagccggaa
     4141 ctagcgatga ttgattcaag aatcggcaaa acaaatcttc atgttccaaa tgatgtaata
     4201 gttgatgcat caatgcccaa catagttcgc gatggcggaa aaatgtggaa taagaaagat
     4261 gaacttcagg attgcattgc aatgattcct gatcgcagtt atgcaactat atatcagaca
     4321 attatagaag atgttaaaac aaacgggcag tttaatcccg caactatggg cgctgtttca
     4381 aatgttggtt tgatggcgaa gaaagcagaa gaatacggtt cgcatgataa gacttttgaa
     4441 gccacgggta atggggtcat ccgcgttata aactccgaag gaaaaacttt gctggagcag
     4501 agggttgaaa cgggagatat tttcagaatg tgccaggcga aagatgaagc gataaaggat
     4561 tggattaaac ttgcagtaaa aagagcaaaa gcatcttcaa ctcctgcagt tttctggctg
     4621 gatgaaaaca gagcacacgc caaagaatta attgtaaaaa tcaaaaagta tttaactgaa
     4681 cttgatacaa ccggactgga gataaaaata cttaaacctg ttgacgcaat gaagtacact
     4741 ctagaaagaa cacgaaaagg attagataca atttctgtaa ccggaaatgt tcttaga
//
LOCUS       DASWBY010000256         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_11536, whole genome shotgun sequence.
ACCESSION   DASWBY010000256 DASWBY010000000
VERSION     DASWBY010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11536"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..647)
                     /gene="ilvN"
                     /locus_tag="VF870_04670"
     CDS             complement(117..647)
                     /gene="ilvN"
                     /locus_tag="VF870_04670"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX9251509.1"
                     /translation="MKEQYTISIFTENKVGLLHRVTIIFTRRHINIESITASESEVKG
                     IYRYTIVVFETRESVEKLVKQIEKQVDVFRAFYHTTDEIIHQEIALYKIATSSVQNGL
                     GLEKIIRDHHARILSLEQDYVVIEKTGHKEDTKELFDALRPFKVLEFVRSGRVAMMKE
                     IDNFSDTLHELEKSYH"
     gene            complement(663..2417)
                     /gene="ilvB"
                     /locus_tag="VF870_04675"
     CDS             complement(663..2417)
                     /gene="ilvB"
                     /locus_tag="VF870_04675"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX9251510.1"
                     /translation="MKDVMEKKSAKIIEKEKSKTETTEKISGAEAVVKCLIEEGVDIV
                     FGYPGGAIMPVYDALFNYQENVKHILTRHEQGAVHAAEGYARSTGKVGVCIATSGPGA
                     TNLVTGIADAFIDSTPVVCITGQVASPLLGTDAFQETDVVGIVTPVTKWDIQITDANE
                     IADAFAKAFYIAKSGRPGPVVIDITKDAQFQQIDFNYEKCNKIRSYFPKPKIDLSKVE
                     AAAGLINESKKPFLIFGQGVILGHAENELRSFLEKSGIPSAWTLLGLSALPNDHPQNF
                     GMLGMHGNLAPNKLTNECDVLIAVGMRFDDRVTGDVKRYAKQAKIIHIELDAAEINKI
                     IKADVAVHADAKESLSLLKDLVNYNDYSDWINQFKELQKIEYEKIIKHELYPSENGLK
                     MSEVVRIVSEKTDGEAIVVSDVGQHQMIAARYFNHKNTRSSITSGGLGTMGFCLPAAI
                     GAKLGNPDKQVVAIIGDGGFQMTIQELGTILQTNVAVKVIILNNEFLGMVRQWQQMFF
                     DKRYSQTEMLNPDFIKIASGYGLEAQRVTQRDELESAIDEMLNSEAGYVLEVRVEKED
                     NVFPMIPTGASVSDIRLE"
     gene            complement(2441..4123)
                     /gene="ilvD"
                     /locus_tag="VF870_04680"
     CDS             complement(2441..4123)
                     /gene="ilvD"
                     /locus_tag="VF870_04680"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9251511.1"
                     /translation="MKLNKYSKHLTQSDSQPASQAMLYALGLSEADLAKPFVGIASTG
                     FDGNPCNMHLNNLALEIKKSVNSSDLVGLVFNTIGVSDGISMGSEGMRYSLPSREIIA
                     DSIETMVEAHAYDGLITVVGCDKNMPGALMAMCRLNRPSLMLYGGTIRSGCYKGEKLN
                     VVSAFEAWGKKVSGLISEEDYKNVIKNACPGPGACGGMYTANTMASAIEAMGMSLPFS
                     SSNPAESDEKQNECIKVGNAIKVLLEKNIKPSDIITKKSLENTFTLVTVLGGSTNAVL
                     HLIAIAKSAKVDFTFDDFQRITDKTPFLADLKPSGKYLMEDVHQAGGTPALMKYLLEN
                     NFLHNDCLTVTGKTISENLQSVPSLNPTQKVIVPIENPLKESGHIQILFGNLSEQGAV
                     AKITGKEGLKFIGSAKVFNSEFEANEGIEKGLVKKGDVIVIRYEGPKGAPGMPEMLKP
                     TSAIMGAGLGKDVALITDGRFSGGTHGFVVGHITPEAQLGGLIALVKDGDVISIDAEK
                     RTLSLEVEEKEIQLRRSLWKAPELKSKSGWLKKYTQLVSSASEGCVTDEFDK"
BASE COUNT         1230 a          874 c          613 g         1475 t
ORIGIN      
        1 ttcattcttt aaaacttctt tagctttagc taatgagaat tcttctctgg taactacgtt
       61 ttcttcaact ccaccaaaat taattttagc cattttttct ccttaataaa tttttcttaa
      121 tgataagatt tttctaattc gtgcaatgta tctgaaaagt tatcaatctc tttcatcatt
      181 gcaactctgc ctgaacgaac aaattctaat actttaaatg gacgaagagc atcaaataat
      241 tcttttgtat cttccttgtg ccccgttttt tcaatcacga cataatcctg ttcaagggat
      301 aaaattcttg catgatgatc acgaataatt ttttctaaac ctaatccatt ttgaaccgaa
      361 gaagttgcaa ttttataaag agcaatttcc tgatgaatta tttcatcagt cgtatgataa
      421 aatgctctga atacatcaac ctgcttttca atttgtttaa caagtttctc aacactctcc
      481 ctggtctcaa aaacaacaat cgtatatctg taaattccct ttacctcaga ttcagaggca
      541 gtaatacttt cgatgttgat gtgtctccgt gtaaaaataa ttgttacccg atgaagcaaa
      601 ccaactttgt tttctgtaaa gattgaaatt gtatattgtt ctttcattta tattattctc
      661 ctttattcaa gtctaatatc tgatacggaa gcaccagtag gaatcattgg aaatacattg
      721 tcttcttttt caactctaac ctcgagcacg tatcctgctt cggagtttaa catttcatca
      781 attgctgatt caagttcatc tcgttgagtc actctctgtg cttccaatcc ataaccagat
      841 gcaattttta taaaatcggg attcagcatt tcagtttgcg aatatctttt atcaaaaaac
      901 atttgctgcc attgtcttac cataccaaga aattcattgt ttagaattat cactttaact
      961 gcaacattag tttgtaagat agttccaagt tcctgaattg tcatttgaaa cccgccatcg
     1021 ccaataattg caacaacttg tttatcagga tttcctagtt tagctccaat tgctgcaggc
     1081 aagcaaaaac ccattgttcc taagccgccg gaggttatac tgctcctggt atttttatga
     1141 ttaaaatatc gcgctgctat catttgatgc tgaccaacat cagaaactac aattgcttcg
     1201 ccatcagttt tttcagaaac gattctaaca acttcactca tctttaatcc attttctgaa
     1261 ggatacaatt cgtgcttgat gattttttcg tattcaattt tttgcagttc tttaaattga
     1321 tttatccaat ctgaataatc attgtaatta actaaatctt taagtaacga aagagattct
     1381 ttagcatcag catgaactgc tacatcagct tttataattt tattaatttc tgccgcatct
     1441 aattcaatat gaattatttt tgcttgcttt gcgtatcttt ttacatcacc ggttactcta
     1501 tcatcgaaac gcattccaac tgcaattaat acatcgcact cattagtaag tttgtttggt
     1561 gcaagattac cgtgcattcc cagcattcca aaattttggg ggtgatcatt aggaagtgct
     1621 gataacccaa gtaaagtcca tgcagaagga attccggatt tttcaagaaa agatcttaac
     1681 tcgttttcgg cgtgcccaag aatcacaccc tgtccaaaaa ttaaaaatgg tttttttgat
     1741 tcatttatta atccagcagc agcttcaact tttgataaat caattttagg ttttgggaaa
     1801 taacttctga ttttattaca cttttcataa ttaaaatcta tctgttgaaa ttgagcatct
     1861 tttgttatat caataacaac aggacctggt ctgccggatt ttgcgatgta aaatgcttta
     1921 gcaaaagcat cagcaatttc gtttgcatca gttatttgta tatcccactt agtaaccggt
     1981 gttacaattc caacaacatc tgtttcttga aaagcatccg tgcccaacag cggagaagca
     2041 acctgaccag taatacacac tacaggagtt gaatctataa aagcatctgc aattccagta
     2101 acaagatttg ttgcaccagg accggatgtt gcaatgcaaa caccgacttt tccggttgag
     2161 cgtgcataac cttcagctgc atgaactgca ccttgctcgt gtcttgttaa aatatgtttt
     2221 acattttcct gataattgaa gagtgcatca taaaccggca taattgcccc gccaggataa
     2281 ccaaaaacta tatccactcc ttcttcaatt aggcatttta caacagcctc agctcctgaa
     2341 attttttctg ttgtttctgt tttgcttttt tctttctcaa taattttagc agattttttt
     2401 tccatcacat ctttcattca tttctcctac tggataatta ttatttatca aattcatctg
     2461 tcacacaacc ttcagaggct gatgaaacaa gttgtgtata ttttttaagc caaccgcttt
     2521 ttgattttaa ttccggtgct ttccataaag atcttcttag ctgaatttcc ttttcttcta
     2581 cttcaaggct aagtgttcgt ttttcagcat ctatggaaat aacatctcca tctttcacta
     2641 atgctattaa gccacctaat tgtgcctcag gagttatatg accaacaaca aaaccgtgtg
     2701 taccaccaga aaatcttcca tcagtaataa gcgcaacatc ttttcccaat cctgccccca
     2761 taattgcaga agtcggtttt agcatttccg gcattccagg tgcacctttg ggcccttcat
     2821 aacgaataac tataacatca ccttttttta ctaatccttt ttcaattcct tcatttgctt
     2881 caaactcact attaaaaacc tttgcagaac caataaattt taatccctct ttgccggtta
     2941 tctttgctac agctccttgc tcagaaagat taccaaacaa aatttggata tggcctgatt
     3001 ctttaagtgg attttctatc ggtacaataa ctttttgggt tggatttaag gaaggaacac
     3061 tttgaagatt ttctgaaatt gttttacctg ttactgttaa acaatcattg tgtaaaaaat
     3121 tattttcaag taagtacttc atcaatgccg gtgtgccgcc tgcctgatga acatcttcca
     3181 tcaaatactt tccacttggt tttaaatcag caagaaatgg tgttttatct gttattcgtt
     3241 ggaagtcatc gaatgtaaaa tcaacttttg ctgattttgc gattgcgatt aaatgtaaaa
     3301 ctgcattagt agaaccacca agaaccgtaa ccaatgtaaa cgtattctct aaagattttt
     3361 ttgtaatgat atctgaaggt tttatatttt tctccaacag aaccttaatt gcgtttccga
     3421 ctttaataca ttcattctgc ttttcatcag actctgctgg atttgaagaa ctaaacggta
     3481 aactcattcc cattgcttca attgcacttg ccattgtatt tgctgtatac attcctccgc
     3541 aagcaccggg accgggacag gcattcttaa ttacattttt ataatcctct tcagatatca
     3601 aaccagatac ttttttaccc cacgcttcaa aggcagaaac gacattaagt ttttctcctt
     3661 tataacatcc agaacgaatt gttcctccat aaagcattaa cgaagggcga tttaatctgc
     3721 acattgccat caaagcgccg ggcatatttt tatcacagcc aactactgta attaatccat
     3781 cgtaagcgtg tgcttcaacc attgtttcaa tcgaatctgc aataatctca cgcgacggaa
     3841 gtgaatatct cattccctct gaacccattg agattccatc actaactcca attgtattaa
     3901 aaactaatcc gactaaatca gatgagttaa ccgatttttt aatctctaat gcaagattat
     3961 tcaaatgcat gttgcaggga tttccatcaa atcctgtact tgcaatccca acgaatggct
     4021 ttgctaaatc tgcctcgctc aaacctagtg cataaagcat tgcttgagaa gcgggctgcg
     4081 aatcgctttg agttaaatgc ttactgtatt tattcaattt cataatattt tattttatga
     4141 tttagtggat aataaaaaag cccttctcgc tgtgagaagg gcttttgtta ag
//
LOCUS       DASWBY010000257         9286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_83862, whole genome shotgun sequence.
ACCESSION   DASWBY010000257 DASWBY010000000
VERSION     DASWBY010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83862"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF870_04685"
     CDS             <1..197
                     /locus_tag="VF870_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251512.1"
                     /translation="VFDENDNEIFQWGPRPIEAQNLFHKLKNEKIAKPDINKELHLWY
                     GRNRGKEVEKEISDLVKKSL"
     gene            complement(207..1634)
                     /locus_tag="VF870_04690"
     CDS             complement(207..1634)
                     /locus_tag="VF870_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9251513.1"
                     /translation="MFAKIIKYFKTTRFKTTLWYSIVFLLLEVVIGLVIYGFLKHNLY
                     KELDLSLTKQADMIYHFVEESHVNLMDFQADSVYNSPNDLIYDLIFEVFAFNTNNTFV
                     QVSYKDQTVFSTVNLKNITIKNPDSLEVKSGIYSFSDSLLSENTIRAAYLNKDDYKII
                     VAFPTYVINQTLKNLTDIYIIIAPVFFLLSLIGGAVISFRALSRIDKIIAETNAITAQ
                     NLNKIIEGSEYDDEYGRLVTTMNNMINRIKTSIEYMNHFSISASHELKTPLTILRGEI
                     ELALKSPKTPEQYRETLQSNYEETIRLINIVEHLFYLSKIDNAIVRLNKQTIELESFI
                     KSVADNFLNLAKEKNIKINLDFAGGKKIKVEADPELFKQVVINLVENAIKYGNESSDF
                     DIICKSTNDGSVSLSFLNYAEAIPKETLSRLFERFYRVESSRNRGLGGVGIGLAIVKS
                     IVDLHNWKINVKTENNVKIIFTIII"
     gene            complement(1667..2338)
                     /locus_tag="VF870_04695"
     CDS             complement(1667..2338)
                     /locus_tag="VF870_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9251514.1"
                     /translation="MRILVVEDEKKVASFIKKGLEEDYYSVDIASDGREGSRLAYSDE
                     YDLIIMDIMLPFKDGITILKEIRQEKITTPVLMLTAKDDVADKVAGLDSGADDYLPKP
                     FAFDELLARIRALLRRNNTEKNIILKISDLELDTQSHKAFRSGKEIFLTQKEYSILEY
                     LMRNKNRVISRVKLSEHVYDYHFDSDTNVIDVYINKLRNKIDKGFENQILFTVRGVGY
                     MIKEN"
     gene            complement(2406..2747)
                     /locus_tag="VF870_04700"
     CDS             complement(2406..2747)
                     /locus_tag="VF870_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251515.1"
                     /translation="MKTRSFALVLSILFLAFTVNTAMMAQSNNTKNTNTKSKTTVNKT
                     ETNKTEVKQPATTTTHHSTTTQTKESVDKVNKDVKSKNEKDMKKAESEMKNNETMTKN
                     TEKKSSEKTKK"
     gene            complement(2857..3198)
                     /locus_tag="VF870_04705"
     CDS             complement(2857..3198)
                     /locus_tag="VF870_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9251516.1"
                     /translation="MEYYNNLSQQPPKQLKFEEIFAFYYNLKGASNADDGKFDEAIKN
                     FDKAIELNPELSSALFNRATIKADLGDLKGAREDFVRVREIELKHNTDLYQNYVTTYF
                     TKKTKGRIKIF"
     gene            3648..6008
                     /locus_tag="VF870_04710"
     CDS             3648..6008
                     /locus_tag="VF870_04710"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9251517.1"
                     /translation="MKKFLLLFIVSIFILQTNVLPQSPVVQQIINSVNQDSLIYFVRE
                     LSGNVPTIINGTSQTILSRNKNQPGNALAETYIKQKLQYYGLTTSIQSFSTTGKNVYA
                     VQPGTEFPNKKYIICAHYDDMPTGTTAPGADDNASGTAAVIEAARIFSQYSFPFTIVY
                     ALWDEEEQGLIGSEYYATQAANAGDSILGVINLDMIAYDGNGDGNADVHNSAVANTST
                     LKDKMLEVNLVYGINLDLDVVPSQPYSDHASFLNHGYGAILLIEDDNDFHPQYHTVND
                     NLSYYNQPYYVKMSKLAFGTLSTLSLNLNLDIQHTPIASITVSEPILTTAFVSSGLQI
                     GTGSLAPRLYYRTRISGGTFGNFIEVIGAPTESANYTFSIPALPLGTVCQYYIAAQDA
                     NSSIVKTLPVGGGGFNPPGSTPPPSFYQFFVAPLTVAMYDEANNINNWTSTSGWNITT
                     AKYVSAPSSFTDSPGGNYVANTTSSLRYNNQVALTNVLGAVLEYDTQWDIESDWDYGQ
                     IQLSTNNGTNWISLAGQYTNTGTGSFQPPNEPLYDGTQSTWVHESIDISAYAGQQITI
                     RFYFRSDLSIQGDGWYVDNVKVSVYSGVIPVELVSFTSSVINNTVGLNWITATELNNS
                     GFEIQRSGHTGPSKSVKNWESIGFVTGNGTSTEVHSYSFLDQNPIAGTSFYRLKQIDF
                     DGTSEYSNIIEVFYGSVAEFALEQNYPNPFNPTTKINYSIKEKGNVELKIFDLLGSEI
                     ATLVNEEKAPGNYEVFFDASRLSSGVYLYTIKSGSFVQTRKMMLMK"
     gene            6161..7447
                     /locus_tag="VF870_04715"
     CDS             6161..7447
                     /locus_tag="VF870_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9251518.1"
                     /translation="MSEKQSSKTGFKSFPLNYWVVIMMEFFERGSYYGMMSILSVYMT
                     DQLSYTKESVGIIKSVIQPMLYILPILSGAIGDRFGYKRVLTFAFIFLGLGYFLTSQT
                     TEYAMVFGSLIIMAVGAGAFKPMISGTIARSTNEGNSTLGFGIFYWSINLGAFLFPLI
                     LVPYIKNTFGWEYVMMASAIATASMLIPTFLIYKEPKRPESTKTLAEVLKGAVMVLTD
                     FKFIGLIVIYSGFWILYFQMFDSVLWYVQKYVDATSLNNFVNGALGGIGININWKFDV
                     EHVTVINAGTIILLQIAVSNIVKHTKALPTMIAGIAMGTIGMAILAINTNIWVFMLGI
                     TIFSIGEMTAHPKFISYVGLIAPEDKKALYLGYSFLYGVLGSFIGGILGANLYVHFVD
                     NLNQPATLWIIFSLIGVITIIGLLLYNKFVSPRKPV"
     gene            7670..>9286
                     /locus_tag="VF870_04720"
     CDS             7670..>9286
                     /locus_tag="VF870_04720"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEX9251519.1"
                     /translation="MIEAGAKLKFILFIDALLFLLCLIGLISISQKAQLPFEITAQDS
                     FLSIQIQNSNPYGLKTGDKLISVDGFNVKLVDKTEFITDRKNIGEKISITVQTNSGIK
                     NLSVVLTNYYSAFYVTSTSIVALFFFVIALFVILKKSEMKASYIFHWASISMSIMLCL
                     TWSNLNTFGFVSKYFFRTVLHISYTLTPALFVHFSLIFPRENTYKWQKLLKTNYLIAF
                     VLVVLNSYAFFYALSVLTDNSINNYLYVFNIIRIYLIISVIISILFFILALIKETGKV
                     ERKQLKWLLFGFIIGPLSFVILWVLPILLSGEALIPEEIVMILICAIPVTFAIAIIKY
                     HLLDIDEVLNRSIVYGIVISILLILYSVTIGIFISSSHIFDQAIISAAAAVILALLFQ
                     PIKSKVQKFVDKKFFRIQYNFRKELNRFTAQIKNYNDVYSLSEYLIKEINTLIPADKI
                     AFGELDTQTGKLFIRTQINFDQIASKILRIKPETLERKWFQVAAVKNKVEGEANISTI
                     FQNTLVRWKINLVVPIKSVNDELFGFIILGN"
BASE COUNT         2982 a         1525 c         1491 g         3288 t
ORIGIN      
        1 ttgtgtttga tgaaaacgat aatgaaattt ttcaatgggg tccccgtccg attgaagcac
       61 aaaatctttt tcacaaatta aaaaatgaga aaatagcaaa accagatatt aataaagagt
      121 tgcatttatg gtacggaaga aatcggggca aagaggttga aaaagaaatt tctgatctgg
      181 taaaaaaatc tttgtaaaat tctgaatcaa ataattattg taaaaattat ttttacgtta
      241 ttttcggttt ttacattaat tttccaattg tgcaggtcca cgattgattt aacaattgca
      301 aggccaatgc caactccgcc gagtccgcgg ttgcgcgatg attccaccct ataaaatctt
      361 tcaaaaagtc ttgataaagt ttcttttggt atagcttctg cataattcaa aaaacttaaa
      421 gagacactgc catcatttgt acttttacag attatatcaa aatctgagga ttcatttcca
      481 tatttaattg cattttcaac caggttaata actacctgtt taaatagttc cggatctgcc
      541 tcaactttaa tttttttacc tccggcaaaa tctaaattta ttttaatatt tttttcttta
      601 gccagattta aaaaattatc agcgacactt tttataaatg attccaactc aatagtttgt
      661 ttatttaacc ttacaatcgc attatcgatt ttagataagt aaaaaagatg ttctacaata
      721 tttattagtc ttatggtttc ttcataatta ctttggagcg tttcccggta ttgttccgga
      781 gtttttggcg attttaatgc aagttcaatt tcaccgcgta aaattgttaa aggagtttta
      841 agttcgtgcg aggctgaaat ggaaaaatga ttcatatatt caatcgaagt tttaatacga
      901 tttatcatat tgttcattgt tgtaacaagc cgcccatact catcatcata ctcgcttccc
      961 tcaataatct tatttagatt ttgcgctgtt atagcgttag tctctgcgat aatcttatca
     1021 attcttgaaa gcgcccggaa agaaataacc gcaccgccaa ttaaagaaag taaaaagaat
     1081 accggggcta taattatata aatatctgtt aagtttttaa gtgtctggtt tataacatat
     1141 gtcggaaaag caacaattat tttgtaatca tctttattta agtacgccgc tcttatcgta
     1201 ttctcagata ataaagaatc agaaaaggaa taaatgcctg attttacttc taatgagtcg
     1261 gggtttttta tagtaatgtt tttaagatta acggtactga atacagtttg atctttataa
     1321 ctgacctgaa caaaagtgtt gtttgtgttg aatgcaaata cttcaaatat taaatcatat
     1381 ataagatcat ttggagaatt gtaaacagag tctgcttgaa aatccatcaa attaacatga
     1441 ctttcttcta caaaatgata tatcatatca gcctgtttgg tcagcgataa atcaagttct
     1501 ttatacaagt tgtgtttaag aaatccataa attacaagac caataacaac ttcaagcaac
     1561 aggaaaacaa tggaatacca caaagtggtt ttaaatctgg tggttttaaa gtatttaata
     1621 attttagcaa acattacaac agcgattgga taatctggct taacgtttaa ttttctttta
     1681 tcatataacc aacaccccgc acagtaaaaa gaatttggtt ttcaaatcct ttgtcaattt
     1741 tatttctaag cttatttata tagacatcaa taacgtttgt atcagaatca aaatgatagt
     1801 cgtaaacatg ctcggataat tttactcttg atataacccg atttttattc cgcattaaat
     1861 attcaagtat tgaatattct ttttgagtta agaaaatttc ttttccgctt ctgaatgctt
     1921 tatgtgattg tgtgtctaat tccagatccg atatttttag aatgatattt ttttcagtgt
     1981 tatttcttct aagcagggct cttatccggg ccagcaactc atcaaacgca aaaggctttg
     2041 gcaaataatc atcagcaccg gaatccaatc cagcaacttt atccgcaaca tcatcttttg
     2101 cagttaacat taaaacggga gtagttattt tttcctgccg gatttcttta aggatggtga
     2161 taccgtcttt aaaaggaagc ataatatcca taataataag atcatactca tcagaatatg
     2221 ctaacctgct gccttctctt ccatccgaag caatatcgac agaataataa tcttcttcta
     2281 atcctttttt aataaaggat gctactttct tttcatcttc aactacaaga attctcatat
     2341 ataaaattaa taaaaaaaaa ggagagaata tctctctcct tctaaaataa tctctttaag
     2401 gaaatttact ttttagtttt ttcacttgat tttttctccg tgtttttggt catcgtttca
     2461 ttattcttca tttcgctttc tgctttcttc atatcctttt cattcttgga ctttacatct
     2521 ttgttgactt tatctacact ttcttttgtt tgagtagttg tactatggtg ggtagttgtg
     2581 gttgctggtt gtttaacttc ggttttattt gtctctgttt tgtttaccgt agtcttactt
     2641 tttgtgttag tgtttttagt attgttgctt tgtgccatca ttgccgtatt aacggtaaat
     2701 gccaaaaata agattgaaag tacaagtgcg aatgatcggg ttttcatatt gagttccttt
     2761 ataatttgtt tttgaattat ttattgagtt tccaagtttc ttaaaactta cagagtaaac
     2821 ttatttttta atcattaaga ttagcttaaa tatttattaa aatattttaa tccttccttt
     2881 agttttttta gtgaaatacg tagttacata gttttgatat aaatctgtat tatgttttaa
     2941 ttctatctct cttaccctca caaaatcttc tctggctcct ttaagatcac caagatcggc
     3001 tttgatagta gcacgattaa ataaagctga acttaattca ggatttaact caatagcttt
     3061 atcaaaattc ttgatagcct catcaaactt accatcatct gcattgcttg ccccttttag
     3121 attataatag aacgcaaata tttcttcaaa cttaagttgt tttggaggct gctgggataa
     3181 gttattgtag tattccattt ttagttcctt ttgattttgg tctcactatt ttatgaagtt
     3241 ttaactcaca atcatttttt atttaccacc aaatatattt ttgttagatt aacaccagat
     3301 taatgaaaga ttaaaaaaaa ttaatgtggg atggtttttc actgatgata ataataggac
     3361 aaatcttcaa tatatatgaa gttaaaaatt ggcagcattt aaccatcaaa aaattatatc
     3421 caaattttga gaatagtctg catctatttt agcctctttt gagaaaaagc tgaatatatg
     3481 atccattatt tgatgataat aaacaaaaac acagcattta aaagttagta gattagcata
     3541 atttttgcac ttaattaacc tttagttttg cctggtaatt gttgtcaact cttatataga
     3601 aatttttatt tagttaactt atcatttaac caacttagga tactgctatg aaaaaatttt
     3661 tactgctttt tattgtctca attttcattc tgcagacaaa tgttcttccc caatcacctg
     3721 tagttcaaca aataataaat tctgttaatc aggattcact tatatatttt gttcgtgagt
     3781 tatcggggaa tgttccgaca ataattaatg gaacatcgca aacaatttta tccagaaata
     3841 aaaatcaacc cggcaatgcg ctggcagaaa cttacattaa acaaaaatta cagtattacg
     3901 gattaacaac aagcatccaa tcttttagca ctacaggtaa aaatgtttat gcagtgcaac
     3961 ccggcacaga atttccgaat aaaaaatata ttatttgtgc acattatgat gatatgccta
     4021 ccggtacaac cgcacctggg gctgatgaca acgcaagcgg aactgcagca gtaatcgaag
     4081 ctgcaagaat attttcgcaa tactcttttc cttttacaat agtttatgct ttgtgggatg
     4141 aagaagaaca aggtttaatt ggaagtgaat actatgcaac tcaggctgca aatgctggag
     4201 attcaattct tggcgttata aatcttgata tgatagctta tgatggtaat ggtgatggca
     4261 atgctgatgt acataacagc gcagttgcaa ataccagtac ccttaaagat aaaatgcttg
     4321 aagtaaattt agtttatgga attaatcttg atctggatgt tgttccatca caaccctaca
     4381 gtgatcatgc ttcattctta aatcatggtt atggtgcaat tcttttgatt gaagatgata
     4441 atgattttca tccacagtat catactgtaa atgataattt atcctactac aatcagccat
     4501 actatgtaaa gatgtcaaaa cttgcctttg gtacactttc aacattatca ctaaacctca
     4561 atcttgatat acagcatact ccaattgctt ctataacggt atctgaacca atattaacaa
     4621 ccgcatttgt ttcttctggc ttacaaatcg gaacgggaag tttagcacca agattatatt
     4681 atcgaaccag aatttctgga ggtacatttg gtaatttcat agaagttatt ggcgctccga
     4741 cagagagtgc aaattatact tttagcattc ctgctctgcc attaggaacc gtttgccaat
     4801 attatattgc agcacaggat gctaattcat caatcgtaaa aacgttacct gtaggcggcg
     4861 gtggatttaa tcctccgggc agtacacctc caccgagttt ctatcagttc tttgtggcac
     4921 ctcttacggt tgcaatgtat gatgaagcta ataatatcaa taactggact tctacaagtg
     4981 gttggaatat tacaacggca aaatatgttt ctgctccatc ctcgttcaca gattctcccg
     5041 gaggaaatta tgtcgcaaat acaacttcat cactcagata taataatcag gttgctttaa
     5101 caaatgtact tggcgctgtt ttagaatatg atactcaatg ggatattgaa tccgactggg
     5161 attatggtca aatccaacta tcaacaaaca acggaacaaa ttggatttca ttagcaggcc
     5221 agtatacaaa taccgggact ggctcattcc agccgccaaa tgaaccactt tacgacggga
     5281 cacaatctac ctgggtacac gaatcaattg atatttcagc atacgcaggt cagcagatta
     5341 ccattagatt ttattttcga tcagatttat caattcaggg tgatggctgg tatgttgata
     5401 acgttaaggt ttcagtttac agcggtgtaa ttcctgttga acttgtttca ttcacctcat
     5461 ctgtaattaa taatactgtt ggattaaatt ggataacagc aacagaattg aacaattccg
     5521 gttttgaaat acaaagaagc ggacacaccg ggccaagtaa aagtgtgaaa aattgggaaa
     5581 gtattggctt tgtaaccggc aacggaactt ctacagaagt tcactcttat tcttttcttg
     5641 atcagaatcc gattgcagga acttctttct acagattaaa acaaattgat tttgatggca
     5701 cttccgaata ctctaacatc attgaagttt tttacggatc cgttgcagaa tttgctttgg
     5761 agcagaacta tccaaatcca tttaacccga caaccaagat taattattct ataaaagaaa
     5821 aagggaatgt tgagttaaaa atatttgatt tacttggaag tgaaattgca acattagtta
     5881 acgaagaaaa agctcctggc aattatgaag tattctttga tgcttcaaga ctttcgagtg
     5941 gtgtttattt gtacacaatt aaatcaggca gttttgttca aacacgtaaa atgatgttaa
     6001 tgaaataatt tcaattgtca ctctgagcga aaccgaagag tgaataagta tatctcattt
     6061 gtaaggcacg agcaatcgtg ccttttttat tatatcttaa ctcacatttt cttagctttg
     6121 cattcaataa ataaaatctg aaaactaaaa aggacagcag atgagtgaaa agcaatcatc
     6181 aaaaacagga ttcaaatcct tcccattgaa ttattgggtt gtaataatga tggagttttt
     6241 tgaacgtggc tcctactatg ggatgatgag tatcctttct gtttatatga ccgatcaact
     6301 cagctacact aaggaaagtg taggtattat aaaaagtgtt attcagccga tgctttatat
     6361 tcttccaatt ctttcgggtg caattggcga ccgctttggg tacaaaagag tattaacttt
     6421 tgcatttatc tttttagggt tgggatattt tcttacaagc caaaccacag aatacgcaat
     6481 ggtgtttggc agtttgataa ttatggctgt tggtgccggt gcatttaaac caatgatatc
     6541 cggaacaatt gctcgatcaa caaatgaagg caacagcaca cttggttttg gaatttttta
     6601 ttggtcaata aaccttggtg cttttttgtt cccgctgatt cttgttccat atattaaaaa
     6661 tacttttggc tgggaatatg ttatgatggc ttcagcaatt gccaccgctt caatgttaat
     6721 tccaactttt cttatataca aagaacccaa acgacctgaa agcacaaaga cgcttgcaga
     6781 agttttaaaa ggtgctgtga tggtcttaac tgattttaaa tttattggat tgatagtaat
     6841 ttattcgggt ttctggattt tatatttcca aatgtttgat tccgttctct ggtatgttca
     6901 aaaatatgtt gatgcaactt ccctaaataa ctttgttaac ggtgcacttg gcggcattgg
     6961 aattaatatc aactggaagt ttgatgttga acacgtaact gtaataaatg caggtacaat
     7021 tattctgctt caaattgctg tttccaatat tgtaaagcat acaaaagcat taccaaccat
     7081 gatcgctgga attgcaatgg gcacgattgg aatggctata cttgcaatca atacaaacat
     7141 ttgggttttt atgcttggca ttacaatttt ttcaataggc gaaatgaccg cacatccaaa
     7201 gtttattagt tatgttggat tgatagcccc ggaagataaa aaagcacttt atcttggtta
     7261 ctcattttta tatggtgtat taggaagttt tattggcgga atacttggcg caaatcttta
     7321 tgtccatttt gttgacaacc ttaaccaacc agcaacactt tggattattt ttagtttaat
     7381 tggtgttata acaataattg gacttttact ttataataag tttgttagcc caaggaaacc
     7441 tgtttaaatc aataaatgaa taaaaggctg tcttataaat tggaattttt ttgtcattct
     7501 tgtaaaaacg ggaatccaat acatttaaaa caatatggat tccttatctc ctgggaatga
     7561 caaatcagat tattttcaaa atctcatttc tttacaaatg aaaaagattt acttttaagt
     7621 agtgcttaac taaaaacttg catcacaacc ttgtttaaac gaggaataaa tgattgaggc
     7681 tggtgctaaa ttaaagttta ttctttttat tgatgctcta ttatttctat tatgtttaat
     7741 aggcctaatt agcattagtc aaaaagcaca acttcccttt gagataaccg cacaggattc
     7801 ttttctttct atacaaattc agaattctaa tccttatgga ttgaaaaccg gcgacaaact
     7861 aatttccgtt gatggattta atgttaaatt ggttgataaa acagagttta tcacagacag
     7921 aaaaaatatt ggggaaaaga tttcaataac tgttcaaacc aattctggaa taaaaaatct
     7981 ttctgtggta ctcactaatt attatagtgc tttttatgta accagtacat ccattgtagc
     8041 actgtttttt ttcgttattg cattatttgt tatacttaaa aaatctgaaa tgaaagcttc
     8101 ctacattttt cattgggcaa gtatcagtat gtcgataatg ctttgtctga catggtcaaa
     8161 tctcaataca ttcggttttg tatcaaagta tttttttaga actgtacttc atatttccta
     8221 tactctaacg cctgcgctct ttgtgcattt ctcattaatt tttccaagag aaaatacgta
     8281 caaatggcaa aaactgctta agacaaatta tttaattgca ttcgtgttag tggttttaaa
     8341 cagctatgct ttcttttatg ctttatctgt tcttactgat aattcaataa acaactattt
     8401 atatgtattt aacattataa gaatctattt aattattagt gttataattt ctattttatt
     8461 ttttatactg gccttaataa aagaaacagg taaagtagag agaaagcaat taaagtggct
     8521 tctatttgga tttataatag ggccattaag ttttgtgatc ctatgggttt tgccgattct
     8581 tctttccggt gaagcactga taccagaaga aattgtaatg atattaatct gtgctatccc
     8641 ggttacattt gcaatagcta taattaaata ccatctgctg gatattgacg aagtacttaa
     8701 tcgaagcatt gtatatggaa ttgtgatttc tatccttcta attttatact ctgtaacgat
     8761 tggtatattt atttcttcat cccatatttt tgatcaggca attatatccg cggctgctgc
     8821 tgtgattctt gctctattat tccagccaat taaatccaaa gttcagaaat ttgtagacaa
     8881 gaaatttttt agaattcaat ataattttag aaaagagtta aacagattta ccgcacaaat
     8941 taaaaactac aacgacgttt attcacttag cgaatattta attaaagaaa taaatacttt
     9001 aattcctgca gataaaattg ctttcggtga acttgataca caaacaggaa aactatttat
     9061 tcgcacacaa atcaattttg atcagattgc aagcaagatc ttgcgaataa aacccgaaac
     9121 attagaaaga aaatggtttc aggttgccgc tgttaaaaac aaagttgaag gtgaagcaaa
     9181 tatttcaact atttttcaaa acacgttggt acgatggaaa ataaatcttg ttgtacctat
     9241 taaatctgta aacgatgagt tatttgggtt tataattctt ggaaat
//
LOCUS       DASWBY010000258         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_271730, whole genome shotgun sequence.
ACCESSION   DASWBY010000258 DASWBY010000000
VERSION     DASWBY010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271730"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VF870_04725"
     CDS             <1..782
                     /locus_tag="VF870_04725"
                     /inference="COORDINATES: protein motif:HMM:NF015817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HEX9251520.1"
                     /translation="KSGTSIVIANLKEKYKLNTIEKQLGKKFTILPIPSGREICEKQL
                     FHLIDKVEKVEVDTVQVEPYLPKIYEKLEWMDRNDLIQRFVSIEFNRFLEYYKNMADL
                     NSPSEGKFKDRDRERPSRGGNNNYTRYFINLGVTDGLKPTQLIGMINDFTGMRDIAVG
                     EIEILKNFSFFEADTNFQKEIIEGFKGQKLKKREINLEIAEKRRGGGSGGGSKPRFNS
                     DRKSSDGFKKSGGFSRDKKDFGDKKDFKRKSSFSRDRKSRD"
     gene            complement(914..>2663)
                     /locus_tag="VF870_04730"
     CDS             complement(914..>2663)
                     /locus_tag="VF870_04730"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9251521.1"
                     /translation="GQNYWQNKSDYNIKVNLDPKTKILQGSEDIVYYNNSPDSLSQIV
                     VRLYHNISKPNARRDFYLNENAITEGVQLKKIFVNDNEIEVSNKSQVNVTGTNLSIKL
                     AEKLVPNSTINLSFDWEIQLPSVESIRYGSYDSTSMFVAYWYPQVSVYDDIDGWDRQD
                     YAGTLEMYNDFNNYNVEFTVPAGFQIWATGVWQNPKEILNQKYFDRYNEAWKSDDVVK
                     IIEQADLAGNDIYKTTDGYQTLKFKADHVPDFAFGISDHYLWDAVSFEVDKKSGRRVY
                     CAAAYKKTSNDFVDVAYYAKETLKYFSFEMPGVLYPYPSATVFNGSGGMEYPMIVNNG
                     SENSKSGTVGLTSHELAHQYLPFYMGTNERKYPFMDEGWAVMLPYDFQERMAKGNTPR
                     IRTVKGYEDFAGNEFDLPIMVPSPTISWRSYRISAYNKPAIAYDILRKTLGDELFLKA
                     LHTYMERWNGKHPIPADFFFTFNDVTKQDLSWYWKPWFYNFSYPDLSIEKVKSGKNSL
                     AVEVKKFGSLPLPVKLQVMYKNEVLKEIYKNADVWKSGKEKIEVKIDGVKNFDAVILG
                     SQQIPDVNSINNVYFK"
BASE COUNT          889 a          468 c          448 g          858 t
ORIGIN      
        1 gtaaaagcgg aacttcgatc gtcattgcaa atcttaaaga aaaatataag ctcaatacta
       61 ttgaaaaaca gttaggtaaa aagtttacaa tcctacccat tccttcgggc cgagagattt
      121 gcgaaaaaca actttttcat ctgattgata aagtagaaaa agttgaagtt gacacagtac
      181 aggttgaacc atatctccca aagatttatg aaaaactaga atggatggac aggaatgatc
      241 ttattcaaag atttgtatcg attgagttca atagattttt agaatactat aaaaatatgg
      301 ctgacctcaa cagtccaagt gaaggaaagt ttaaagacag agatagagaa agaccatcac
      361 gaggcggtaa taataactac acaagatatt ttataaatct tggggtaacg gatggtttaa
      421 aacctacgca gttaatcggt atgataaacg attttaccgg aatgcgtgat attgcggttg
      481 gtgagattga aattctaaaa aacttttcat tctttgaagc agatactaat tttcaaaaag
      541 aaattattga aggctttaag ggtcagaaat taaagaagcg tgaaataaat ctagaaatag
      601 ctgaaaaaag acgcggtggt ggcagtggag gcggaagtaa acccagattc aattcagata
      661 gaaaaagttc cgatggtttt aaaaaatccg gtggtttctc cagagataaa aaagatttcg
      721 gtgataagaa agattttaaa cgtaagagta gtttttcaag agatagaaaa agcagagatt
      781 agtagttcat tttatgaggt tattctataa atcgatattc atctgtcatt cccgtgaaaa
      841 acgggaatgg caaatatttt taatcggatt ttaaaacatc ctcaaaaata tatttgagtg
      901 tttttaatga tccttattta aagtaaacat tatttattga gttcacatcc ggaatttgct
      961 gactgcccaa tattacggca tcaaagtttt taactccatc gatctttact tcaatttttt
     1021 ccttacctga tttccagaca tcggcatttt tataaatctc tttaagaact tcatttttgt
     1081 acataacctg taatttaaca ggtagcggca agctgccaaa ttttttaact tcaaccgcaa
     1141 gagaattttt tcctgattta actttttcga tcgatagatc cggatagctg aaattataaa
     1201 accatggttt ccagtaccag cttaaatcct gcttagttac atcattaaag gtaaagaaaa
     1261 aatcagcagg tatagggtgt tttccattcc atctttccat ataagtgtga agagctttta
     1321 agaagagttc atcccctaaa gtttttctaa gtatgtcata ggcaattgca ggtttgttat
     1381 atgcagaaat tctgtatgat cgccaactga ttgtaggtga tggaaccatg attggtaaat
     1441 caaattcatt ccccgcaaaa tcttcataac ctttaactgt acgtattctt ggtgtattgc
     1501 ctttggccat tctttcctga aaatcatagg gaagcattac ggcccaaccc tcatccataa
     1561 atggatattt tcgttcattc gttcccatat agaatggcaa gtattgatgc gcaagttcgt
     1621 gagaagttaa tccgacagtt ccggacttgg aattttcact tccattatta actatcatcg
     1681 gatattccat gccgccggag ccattaaata ctgtagcaga tggataagga taaagaactc
     1741 ccggcatctc aaaagaaaaa tatttaagag tttcctttgc gtaataggca acatcaacaa
     1801 aatcattact tgttttctta taagcagccg cacaataaac tcttcttccg gattttttat
     1861 ctacttcaaa actaacagca tcccacaaat aatgatcgct gataccaaat gcaaaatcag
     1921 gaacatggtc agctttaaat tttaatgttt gatatccatc tgtagtttta taaatatcat
     1981 ttcccgctaa atcggcttgc tcaattatct taacaacatc atcagatttc catgcttcat
     2041 tatatctatc aaaatatttt tgatttagaa tttccttggg attctgccag actcctgttg
     2101 cccaaatctg aaaaccagcg ggaacggtaa actcaacatt gtaattatta aaatcattgt
     2161 acatttccag agttcctgca taatcctgcc gatcccagcc atcaatatca tcataaacag
     2221 aaacttgcgg ataccaataa gcaacaaaca tagaggtaga atcataactt ccatatctga
     2281 tcgattctac agatggcaac tgtatttccc aatcgaaaga aagattaatt gtactattgg
     2341 gtacaagttt ttctgcaagt tttatggaaa ggtttgtccc ggttacattc acttgcgatt
     2401 tattacttac ttcaatttca ttatcattca caaatatttt ttttaattga actccctcag
     2461 taatggcatt ttcattcaga taaaaatccc gccttgcatt tggcttgcta atgttgtggt
     2521 acaatcttac aactatttgg gacaatgaat caggactgtt attataatag acaatgtctt
     2581 ctgatccttg aagaatctta gttttgggat ccagatttac tttaatatta taatcagact
     2641 tgttctgcca ataattttgt ccc
//
LOCUS       DASWBY010000259         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_160937, whole genome shotgun sequence.
ACCESSION   DASWBY010000259 DASWBY010000000
VERSION     DASWBY010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160937"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1402
                     /gene="pepT"
                     /locus_tag="VF870_04735"
     CDS             155..1402
                     /gene="pepT"
                     /locus_tag="VF870_04735"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEX9251522.1"
                     /translation="MFDANYKFTCVDRFLKYVKYDTQSDEESTSFPSSEKQKILSKDL
                     AEELKSLGLKDAHMDEWGYVMATLPSNTTKKVDPIAFIAHVDTSPAVSGKDVNPIIRK
                     NYQGGDIKLENGGWVITVDDNPDLKNMIGFDIITTDGSTLLGADNKAGVSEIIDAVNY
                     LLTHPEVKHGDIKICFTPDEEVGRGTEKIDLKKLGAKYAYTIDGSSRGELETETFSAD
                     AVVIKFYGKNIHPGYAKNIMINSIKIASLFIESLPKDGLSPETTDGREGYVHCGSFNG
                     NEELTTLKFIIRDFETNKLKEYEDFLKKLAEETVAQFPGARMEFQVIEQYRNMKEVLD
                     KHPHVNDYAIIAMERLGIKPIMHPIRGGTDGYRLSFMGLPTPNIFAGEHSFHSQLEWV
                     AIQDMEMAVKTIVEVAKVWEEKA"
     gene            1504..>2688
                     /locus_tag="VF870_04740"
     CDS             1504..>2688
                     /locus_tag="VF870_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEX9251523.1"
                     /translation="MFKSKQKPELMAPAGDWTMLRAAVSNGADAVYFGLDKINMRAKA
                     ANFSVDELPEIVSYCNEHKVKTYLTLNTIVFEEELPDLEEIIIAAKQNGVDRVICSDL
                     AVADLCHKHEFPFCISTQSSISNSLAADVYKRIGAVRIVLARECSLEEIKKIRTKTDL
                     EIEAFIHGAMCIAVSGRCFMSHHLFGKSANRGECIQPCRREYEVYDPAIDKSLIIGED
                     YIMSPQDLCTIEFIDQLIEAGIDSFKIEGRKRAPEYVAKAVSIYRQAIDLYFEGNLTK
                     EKKKELLTELEKVYNRGFSSGFYFGIPSSEEYSGVEGSKATTRKTYIGKVLNYYKEIA
                     VAHILIESGEIKISNNILIMGETTGVLDIILEKMNVNDKEYPTAIRGDEITIKVSSLV
                     RRN"
BASE COUNT          946 a          399 c          545 g          798 t
ORIGIN      
        1 aatccaacaa aagcaaaaaa acttttgggt tggaaagcaa aaacaaagtt tgaagatctt
       61 gttcagataa tgattaaatc tgatatggat aaagttttaa gaagaggtta ttagataata
      121 aataagtttt attcaataaa cgttagaggt aaacatgttc gatgcaaatt ataaattcac
      181 ctgtgtagat agatttttaa agtatgttaa gtacgataca caatcagatg aagaatcaac
      241 ttcttttcct tcatcggaaa aacaaaagat actttctaag gatcttgcag aagaattaaa
      301 atcattggga ttaaaagatg cccatatgga tgagtggggt tatgttatgg caacacttcc
      361 ttctaataca acaaaaaaag ttgatcctat tgcttttatt gcacacgttg atacctcccc
      421 tgccgtaagc ggtaaagatg ttaatccaat tatcagaaaa aattatcaag gcggggatat
      481 aaaattagaa aacggaggtt gggtaattac tgttgatgat aatcctgatc ttaaaaacat
      541 gattggattt gatatcatca caacagatgg ttcaacatta cttggtgctg ataacaaagc
      601 tggtgtttca gagattatag atgcagttaa ttatttatta actcatcctg aagtaaaaca
      661 cggcgatatt aaaatttgtt ttaccccgga tgaagaagtt ggaagaggaa cagagaaaat
      721 tgatttgaaa aaacttggtg caaagtatgc ttacacaatt gatggttcaa gcagaggaga
      781 acttgaaaca gaaacttttt ctgctgatgc agttgtaata aagttttatg gaaaaaatat
      841 tcatcccgga tatgcgaaaa acattatgat taactccata aaaattgcat cgctatttat
      901 tgagagctta ccaaaagatg gattatctcc tgaaacaact gatggacgag aaggatatgt
      961 tcattgcgga tcgtttaacg gcaatgaaga gcttacaaca ttgaagttta ttattcgtga
     1021 ttttgaaact aataaactta aagagtatga agattttctg aagaagcttg ctgaggaaac
     1081 agtagcacaa tttcctggtg caagaatgga atttcaggta attgaacagt acagaaatat
     1141 gaaggaagtt ttagataaac atccgcacgt taatgattac gcaatcatcg caatggagcg
     1201 acttggaatc aaaccaatta tgcacccaat tcgcgggggt acggatggtt accgtttatc
     1261 ttttatggga ttgccaacac cgaatatttt tgccggagag catagttttc actctcagct
     1321 tgagtgggta gctattcagg atatggaaat ggctgtaaag acaatcgtag aagttgcaaa
     1381 agtctgggaa gaaaaagctt aacacaattg cacacggatg acgcggatta aacggattat
     1441 cacagattag atcagtgtaa acccgcgtta tctgttttat ccgcgttcta tccttaggat
     1501 attatgttta aaagtaaaca aaaaccagaa ctaatggccc ctgccgggga ttggacaatg
     1561 ctgcgtgctg ctgttagcaa tggtgctgat gcagtttatt ttggtttgga taaaattaat
     1621 atgcgggcaa aagctgcaaa cttttctgtt gatgaacttc ctgaaattgt ttcgtactgc
     1681 aatgagcata aagtaaaaac ttatcttaca cttaacacta tagtttttga agaagagtta
     1741 cctgatcttg aagaaataat tattgcggca aagcaaaatg gagttgatag agttatttgt
     1801 tctgatctcg ctgttgctga tttgtgccac aaacacgaat tcccattttg tatctccaca
     1861 caaagttcaa tatcaaattc gcttgctgct gatgtttata aaagaatagg cgcagtaaga
     1921 attgtacttg ctcgcgagtg ttcgttagaa gagattaaaa aaataagaac aaaaactgat
     1981 ttagaaattg aagcatttat tcatggtgct atgtgtattg ccgttagcgg caggtgtttt
     2041 atgagtcatc atctttttgg caaaagtgca aatcgcggtg agtgtattca gccttgccgc
     2101 agagaatacg aagtttatga tcctgcaatt gataaatctt taattattgg cgaagattat
     2161 ataatgtctc cgcaggactt atgtactatt gaatttattg atcagttaat tgaagctgga
     2221 attgattcat ttaaaatcga aggaagaaaa cgcgcacccg agtatgttgc aaaagctgtt
     2281 tccatttatc ggcaggccat agatctttac tttgaaggaa atcttaccaa agaaaagaaa
     2341 aaagaattac taacagaatt agaaaaagtt tataaccgcg gattctcatc aggattttac
     2401 ttcggtattc caagctctga agaatactcg ggagttgaag gaagcaaggc aactacaaga
     2461 aaaacatata tcgggaaagt gcttaattac tacaaagaga tagcagttgc acacatttta
     2521 attgaatctg gtgaaataaa aattagtaat aacattttga ttatgggtga aacaaccgga
     2581 gttttagata tcatacttga aaagatgaat gtaaatgata aagaataccc aactgcgata
     2641 cgcggtgatg aaattacaat taaagtatct tctctggtaa gaagaaat
//
LOCUS       DASWBY010000260         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_497386, whole genome shotgun sequence.
ACCESSION   DASWBY010000260 DASWBY010000000
VERSION     DASWBY010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497386"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VF870_04745"
     CDS             complement(<1..555)
                     /locus_tag="VF870_04745"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9251524.1"
                     /translation="MQNQNPTDPYLHVANIYSHLMNFVSYKWWAKYIYSITKSNVVKN
                     PAVLELAAGNCFLADHLSKIYKDYLVTDISKSMLNHSKNKLNKVCCDMTAIPFTKKFD
                     LILTSFDSVNYLLSKKRLLKFFKEANNHLTKNGVLTFDAALESNSYKHQKTATTKGKA
                     KGFNYHRKSIYLPASNIHKNIFTIV"
     gene            complement(533..1534)
                     /gene="pdxA"
                     /locus_tag="VF870_04750"
     CDS             complement(533..1534)
                     /gene="pdxA"
                     /locus_tag="VF870_04750"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX9251525.1"
                     /translation="MTRVVFTCGDVNGIGPEIAVKAFSKIYSKKNNHQLIFVCPKNVF
                     EYYYKITKVSFNYQIVTDPTFSTSQLNVIPLTNNKIKLGYPTKVSGEIAFNSIKKSLE
                     LSDHNFADVLVTAPISKEAFNLAKLNFPGHTELLAKHENTNNYLMLFLSEKIKAGLLT
                     IHSPINKVSKLISKEKIVKAINLLNLTAKRDLKIKNPKIAVLGLNPHAGEKGLIGNEE
                     ISIIEPVIKSLQKNINVYGPFVPDAFWGNKSYKKFDMILGMYHDQVLVPFKLLNFNSG
                     VNFTAGLKLIRTSPDHGTAYDITGMNKADESSILQSYKYAINIFQNRNRSFAESKSN"
     gene            complement(1531..2811)
                     /locus_tag="VF870_04755"
     CDS             complement(1531..2811)
                     /locus_tag="VF870_04755"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX9251526.1"
                     /translation="MKKILLLTFVLVSQLLAQPDFVIERDQIQSGKFFNSEIHFFPAD
                     QGYLVYYSYKISYSQLFFEKKVDVFNAGFSVNLEIKDSAGNIIKRAFDDKKITAENFE
                     TTNSPNTFLQGVISFNLLEGNYKILSIISDQISKRERRIPTADLILNKSKIILNPIVF
                     DQGIINCDGTNSYVLSNNSASIPFNKPNDDLVIPVTDANINSINIKVKRGDKVFIDGE
                     KITESLIANPEIKLCNNKVIITPATDSASIKLFIVRNFSSKLTEGPIQLEIVPDESLK
                     QQQIFNLNVIWIQKPLSLSDPEEAIKLIEIIESKDVVSNIFSSSGDDTEKLYNYWRKQ
                     DPTPETQFNELMNEFYTRVDYCELNFRSLSGNSGAKSDRGKIYIKFGPPDSILRDTDN
                     QDKVVESWTYNKSKLKFVFIDKDGTGKFTLANGQ"
     gene            complement(2909..>4028)
                     /gene="rho"
                     /locus_tag="VF870_04760"
     CDS             complement(2909..>4028)
                     /gene="rho"
                     /locus_tag="VF870_04760"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX9251527.1"
                     /translation="VKDGLTFSKGVLEVLPDGYGFLRSADYNYLPSPDDIYVSPSQIK
                     KFSLRTGDFVSGQVRPPKEGERFFALLRVEAVNGLSPDHIRNRTLFDNLTPVYPTKKL
                     LLESVPGEYSTRIMDMLAPIGKGQRGLIVSPPKSGKTVLLQKIANSITRNHPEVKLLI
                     LLIDERPEEVTDMERSVKAEVISSTFDEPADRHVQVADMVIEKAKRLVEAKEDVVILL
                     DSITRLARAHNIVVPHSGRILSGGVDSNALHKPKRFFGAARNTEDGGSLTIIATALID
                     TGSRMDDVIFEEFKGTGNMELVLNRDLSDRRIFPAIDVNRSGTRREDLLMKEDDLAKV
                     WILRKILSDFNPVEAMEFLLDKVRGTKNNKEFLNNMNS"
BASE COUNT         1247 a          690 c          626 g         1465 t
ORIGIN      
        1 aacaattgta aaaatgttct tatggatgtt acttgccggc aaataaatgc tttttctgtg
       61 atagttaaaa ccttttgctt tcccttttgt tgtcgcagtc ttttgatgtt tataactatt
      121 actttcaagt gcggcatcaa aagtcaatac tccattcttc gtaaggtgat tatttgcctc
      181 cttaaaaaat tttagtaatc gtttcttact tagtaaataa tttacactat cgaatgaagt
      241 taaaatcaaa tcaaactttt tagtgaatgg aatggccgtc atatcacagc agactttatt
      301 caatttattc ttagaatgat taagcatgct cttggaaata tccgttacaa gataatcttt
      361 atatattttt gaaagatgat ctgctaaaaa acaatttcct gcggcaagtt caagaacggc
      421 tggatttttt acgacattag atttagttat agaataaatg tactttgccc accatttgta
      481 gctgacaaaa ttcataagat gagaataaat attggctacg tgtagatatg gatcagttgg
      541 attttgattc tgcaaaggaa cgattcctgt tttgaaatat attgattgca tatttgtatg
      601 attgcagaat gcttgattca tctgctttgt tcattccggt aatatcataa gcagtaccgt
      661 gatctggtga agtacggatt aattttaatc ctgctgtaaa atttactccg ctgttaaagt
      721 ttaatagctt aaatggaact aaaacctgat catgatacat tcctaaaatc atatcaaact
      781 ttttataaga tttattaccc cagaatgcat cgggaacaaa tggtccgtaa acattaatat
      841 ttttttgtaa tgattttatt actggttcaa taatacttat ttcttcattt ccaattaatc
      901 ctttttcgcc agcatgcgga tttagtccaa gcacagcaat ttttggattc tttattttca
      961 gatcacgttt agcagtcagg ttcaacaagt taatcgcttt gacaattttt tctttagaaa
     1021 tcagttttga aactttattt attggtgaat gtattgtaag caatcctgct tttatttttt
     1081 cagaaagaaa aagcattaaa taattattag tgttctcatg cttagctaat aactcagtgt
     1141 gtccgggaaa atttagcttt gcaagattaa atgcttcctt ggatattggg gctgttacta
     1201 aaacatctgc aaaattatga tcactcaact ccaaagattt ttttatagaa ttaaatgcaa
     1261 tttcacctga aactttcgtt ggatatccta atttaatttt attattagtt aacgggatta
     1321 cattaagctg cgaagtagaa aaggttggat cagtaactat ctgataatta aatgaaactt
     1381 ttgtgatttt gtaataatat tcgaagacgt ttttaggaca aacaaatatt agctgatgat
     1441 tatttttttt gctatagatt tttgagaatg cttttactgc tatctcagga ccaattccgt
     1501 ttacatcacc gcaagtaaaa acaacccgtg tcattgtccg ttcgctaaag taaattttcc
     1561 tgttccatcc ttatcgatga acacaaattt taatttagat ttgttataag tccaactctc
     1621 cacaacttta tcctgattgt ctgtatctct taaaatcgaa tcaggcggtc caaatttaat
     1681 ataaattttc ccgcgatcag attttgcacc gctattaccg gaaagagatc taaagtttag
     1741 ttcgcaataa tcaactcttg tgtaaaactc attcattagt tcattgaatt gtgtttcagg
     1801 tgttggatct tgtttacgcc aatagttgta tagtttctca gtatcatcac cgcttgaact
     1861 aaatatattt gatacaacat ctttgctttc aattatctct atgagcttta ttgcctcttc
     1921 gggatcagag agagacaaag gtttttgaat ccagattaca tttaaattga aaatttgctg
     1981 ctgcttcaga gattcatcag gaacaatttc taactgtatt ggtccttctg taagctttga
     2041 agagaaattt cttacaataa ataattttat gcttgcagaa tctgttgcag gtgtaatgat
     2101 cactttatta ttgcataact taatttcagg atttgcaatt aaagactcag tgattttctc
     2161 accatcaata aaaaccttat cacctctttt aactttgata tttatagagt ttatatttgc
     2221 atctgttacc ggaattacta aatcatcatt tggtttgttg aatgggattg atgctgaatt
     2281 gtttgacaga acgtacgaat ttgttccatc acaattaatt attccctgat caaacacaat
     2341 tggatttagt atgattttgg atttatttaa aatcaaatca gcggttggaa ttcttctttc
     2401 acgttttgaa atctggtcag agatgattga tagaattttg taattgcctt cgagtaaatt
     2461 aaaacttatg accccttgca aaaacgtatt tggcgaattt gtagtttcaa aattttcagc
     2521 tgttattttt ttatcatcaa atgcacgttt aatgatattg cctgctgaat ccttaatttc
     2581 taaatttaca ctgaatccgg cattaaagac atcaaccttt ttttcaaaaa ataattgcga
     2641 ataagaaatt ttatatgagt agtaaactaa atatccctga tctgcgggga agaaatgaat
     2701 ttcagaatta aaaaacttac cagattgaat ttgatcacgt tcaattacaa aatcaggttg
     2761 agctaataat tgagaaacta aaacgaacgt taataataat attttcttca atttttaaac
     2821 tcaaactttt aatagattaa ataaattcaa tcagtttaat aaaaccgaaa aaatatttgc
     2881 actggtcttc aattactgaa caattctttt agctgttcat attgtttaag aattctttgt
     2941 tgttcttggt ccctctaact ttatcaagta aaaattccat agcttcaacg gggttaaaat
     3001 cgctaagtat ttttctaaga atccaaactt ttgcaagatc atcttctttc attaacaaat
     3061 cttctcttcg tgttccggaa cgattaacat caatcgcagg gaagattctt ctgtcactta
     3121 aatctctgtt cagcactagt tccatattac ccgtaccttt aaactcctca aagataacat
     3181 catccatacg gctgcctgta tcaatcaatg cagttgcaat gatagttaag cttccgccat
     3241 cttctgtatt tctagcagcg ccaaaaaatc tttttggttt gtgtagtgcg tttgaatcca
     3301 caccaccgga aagaattcta ccgctatgag gaactactat gttgtgcgca cgagctaatc
     3361 ttgtaatact atcaagcaaa ataacaacat cttcttttgc ttcaacaaga cgttttgctt
     3421 tttcaataac catatctgca acctgtacgt gtctgtcagc aggctcatca aaagttgaac
     3481 ttataacttc tgcttttaca gatcgttcca tatcggttac ttcttcaggt ctttcatcta
     3541 taagcaggat taaaagtttc acttcaggat gattacgtgt aattgagttt gcaattttct
     3601 ggagtaacac agttttaccg cttttaggcg gcgatacaat caaacctctc tgccctttac
     3661 cgattggtgc aagcatatcc ataattctgg tagaatattc accgggtact gattcaagta
     3721 ataatttttt agttggataa actggggtaa ggttgtcgaa caaagtacgg tttctgatat
     3781 gatcggggga tagtccattt actgcttcta ctctaagcag tgcgaaaaat ctttctccct
     3841 cttttggcgg tcttacttgt ccgcttacaa agtcacctgt tctaagacta aactttttaa
     3901 tttgtgaagg tgaaacatat atatcatctg gtgaaggtaa ataattataa tctgcagatc
     3961 gtaaaaatcc gtatccatcc ggaagtactt ctaaaactcc cttcgaaaat gttaatccat
     4021 ctttaacg
//
LOCUS       DASWBY010000261         4525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_310886, whole genome shotgun sequence.
ACCESSION   DASWBY010000261 DASWBY010000000
VERSION     DASWBY010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310886"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VF870_04765"
     CDS             <1..1130
                     /locus_tag="VF870_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9251528.1"
                     /translation="VAALIVVLSVFNGFGSLVTSFLMNFDPHLRVEYKVENTENNYEK
                     VRAIIKETPDLKTFAPFVSGKVLAFNSGITQVISLKGIDLKNADELYNIKANILFGSA
                     DLNEKDETPGILIGLRLADHLQVLVGDTVTIISPAGIEKVITQFGMPNTQQFIVGGIF
                     SSQNNEYDESLAFTYLSEAQYLLGYKDNYQGFEIKLNNSDNSFKAKDFLESRLDGNIF
                     EINTWYDFHKELYTVMQIERWVAYILLSLIIAVATFNILGSLSMSVIEKKRDIGILRS
                     MGSKENSILKIFMYEGLMIGLIGTGLGVLLGYFICFLQIQYNIYPLDPTQYKIDSLPL
                     EIRFLDFFVIAGASLFLSFIASLIPAKRAAKVDALQAIKWE"
     gene            1133..1816
                     /locus_tag="VF870_04770"
     CDS             1133..1816
                     /locus_tag="VF870_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9251529.1"
                     /translation="MDIILSSENITKSYQSSKKNKLEVLKSISFEIEANKISVIVGAS
                     GAGKSTLLHLLGGLDRPDSGKVFYEQKNIFDFNNDKLAKFRNENMGFVFQFHHLLPEF
                     TAMENVSIPQMIKGVSLKSASKNSLRLLEIVGLKDRAEHKPAELSGGEQQRVAVARAL
                     ANDPKIIFADEPTGNLDSANSDSIHKLLFDLRDNFKKTFVIVTHNPELMKLADVIFEI
                     KDGVIKQNS"
     gene            complement(1916..2842)
                     /locus_tag="VF870_04775"
     CDS             complement(1916..2842)
                     /locus_tag="VF870_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX9251530.1"
                     /translation="MIIESIPTFDKYLSSHASTICSLNDKLLVAYFAGKYEGHRKNCI
                     WISTKEGNEWDIPRVLFSGKSVLGREIPCWNPVFHSTKEKLYLYFKIGKSPSLWNGFF
                     SESLDDGETWSKPKLLPAGIFGPMRSSPIRIDNYIVSGSSDEKYVSKMHFELSDDGYS
                     WKKVVPICDPALGDCIQPAIIRLSQNKLIAYARSSIGVLVSTNSQDGGNSWEQVVKTE
                     IPNPDSAIDLISVREDFHILVYNRSTKERTPLVMAISSDGVNWNDKIIIEDGKGEYSY
                     PSITISKNGIEISYTSQRKCIKHALIETSELF"
     gene            complement(2895..4232)
                     /locus_tag="VF870_04780"
     CDS             complement(2895..4232)
                     /locus_tag="VF870_04780"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX9251531.1"
                     /translation="MRVTKPSGKPDALILSKVKLDDKFDADYFRNHLAEFISTLKNEA
                     TKNLHIFIPAYSAVKKYFESEEYYYQTFAEGVLLGNYSFDKYKSKKVKSKVLDVYFYS
                     DNEKKLKNAISVADKLITSVNLTRDLQNEPSNNLTPDLFAKRVVSEVKPFGIKTTVFG
                     EDEIRKRKMGGLIAIGQGSTNKPRFIILEYKPTVKSKKNKKNKIKTIALVGKGITFDT
                     GGISLKPAKNMGEMKADMSGAAVVVGTIIAAAKNSLPVHLYGIIPSAENMVSGESIRP
                     GDIIKTSSGKTIEIEDTDAEGRVVLADALHYASQLTPDQIIDLATLTGACVVALGEFV
                     AGVFTKNDAMADNLYKSGMTTYDRVWRLPMWDDYNELIKSELADVANLGSSRWGGAIT
                     AAKFLEHFVDKNIPWTHLDIAGPAMNNSSRSYTKKYMTGFGVRLLFDYLSVRI"
     gene            complement(4248..4430)
                     /locus_tag="VF870_04785"
     CDS             complement(4248..4430)
                     /locus_tag="VF870_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251532.1"
                     /translation="MKVDFMYKINLKTGGSKIIYNPLSVSLKYLIDDKKLAKSLPNLE
                     KIFNIKFSDFQRKTFL"
BASE COUNT         1448 a          764 c          737 g         1576 t
ORIGIN      
        1 gggttgccgc tcttattgtt gtgctatctg tatttaatgg tttcggaagt ttggttacat
       61 cttttcttat gaactttgat ccgcacctta gagttgaata caaagttgaa aacactgaaa
      121 ataattatga aaaagttaga gcaataataa aagaaactcc tgatctaaaa acatttgccc
      181 catttgttag cggtaaagtg ctggcattta atagcggtat tactcaagta atttcgctta
      241 aaggaattga tctaaagaat gctgatgaac tttataatat taaagcaaac attttatttg
      301 gcagcgctga tttaaatgaa aaggatgaaa cacccggaat attaattggt ttaagattag
      361 cagatcattt gcaagtactt gttggtgata ctgtaacaat tatttctccg gcaggaattg
      421 aaaaagttat cacgcagttt ggaatgccta acacacagca atttattgtt ggtggaatct
      481 ttagttccca aaataatgag tacgatgaga gtttagcttt tacttattta agtgaagctc
      541 aatatttatt ggggtataaa gacaactatc agggttttga aattaagtta aacaattcag
      601 ataattcatt taaagctaaa gactttctgg aatcaagact tgatggaaat atttttgaga
      661 ttaatacctg gtacgatttt cacaaggagt tatatacagt tatgcagatt gagagatggg
      721 tcgcttatat tttactttcg ctaatcatag ctgttgcaac atttaatatt cttggttctc
      781 tttcaatgtc agttatcgaa aagaaaagag acatcgggat tcttagatca atgggatcaa
      841 aggaaaactc aattttaaaa atatttatgt atgaaggatt aatgatcgga ttgattggaa
      901 caggtcttgg agttttgctt ggatatttta tttgttttct ccagatacag tataacattt
      961 atcctcttga tccgacacaa tataaaattg attcacttcc tcttgaaata agatttttag
     1021 atttctttgt gattgcaggt gcttcattat ttctttcttt cattgcatct cttataccgg
     1081 caaagcgcgc cgccaaagtt gatgcattac aagcaattaa atgggaataa gtatggatat
     1141 cattttatca tcagaaaata ttaccaagtc atatcaatcc agtaaaaaaa ataaattgga
     1201 agttcttaaa tcaatttcgt ttgaaattga ggcgaataaa atttctgtta ttgtaggcgc
     1261 atccggcgca ggcaaatcca ctctgctgca tttactcggt ggtttggaca gacccgacag
     1321 cggcaaagta ttttatgaac agaaaaatat ttttgatttt aataatgata aacttgctaa
     1381 gttcagaaat gaaaatatgg gatttgtttt tcagttccat catcttctgc cggagtttac
     1441 agcaatggaa aatgtttcta ttccccaaat gataaaagga gttagtttaa aatctgcttc
     1501 taaaaattca ctcagacttt tagaaattgt aggattaaaa gatcgtgcgg aacataagcc
     1561 tgcagaacta tcaggtggtg agcagcagag agttgcagtg gcaagagcat tagcaaatga
     1621 tccaaaaata atttttgctg atgaacctac aggtaatctt gattcagcta atagtgattc
     1681 tatacataaa cttttatttg atttgcgtga caactttaaa aaaacttttg tcatcgttac
     1741 tcacaatccg gaactaatga aacttgccga tgttattttt gaaattaaag atggtgtgat
     1801 aaaacagaat agttgatttt aataattgct attaatttta ccaggtgatt tttttgctta
     1861 gcaaaattaa attattatca aatgataaag aatatgctta ataatgtgag aattattaaa
     1921 atagctcact tgtttctata agtgcatgct ttatacattt tctttgagaa gtatatgata
     1981 tttctattcc attcttactt attgtaatcg aaggatatga atactctcct ttcccatctt
     2041 caataataat tttatcattc cagttaactc catcagagga aattgccata actaacggag
     2101 ttctttcttt tgttgatcga ttatatacta aaatatgaaa atcttctctg acagaaatta
     2161 aatctattgc ggaatctgga tttggtatct ccgtttttac aacttgttcc catgaattac
     2221 caccgtcttg agaattagta ctaactaaca cgccaatgga actacgtgca tatgcaatta
     2281 atttattttg tgacaatcta attattgctg gttgaatgca atcaccaagg gctggatcac
     2341 agatagggac aacttttttc caactatatc catcgtcact tagttcaaaa tgcattttgg
     2401 aaacatattt ttcatcactg ctaccactga caatataatt atcaattctt atgggactgc
     2461 tacgcatggg accaaaaata cctgctggta agagtttagg cttactccaa gtttctccat
     2521 catcaagact ttcagagaaa aaaccattcc aaagactggg cgactttcca attttgaaat
     2581 aaagatacaa tttttcttta gtagaatgga aaacgggatt ccagcatgga atttcccttc
     2641 ctaatacact tttaccagaa aacaatactc ttggaatatc ccattcgttt ccctcttttg
     2701 tagaaatcca tatacaattt tttctatgcc cttcatattt ccctgcgaaa tatgctacta
     2761 aaagcttatc atttaaacta cagatagtag aagcatgact agagagatac ttgtcaaatg
     2821 ttggtatact ttcaattatc ataacttaaa tatgaagtaa cctatttcta gtaaagaagt
     2881 gggcttggat aaattcaaat tctaacagat aaataatcaa acaacaatct tacaccaaaa
     2941 ccagtcatat atttttttgt gtagcttctt gatgaattat tcattgcagg accagcaatg
     3001 tctaaatgcg tccagggaat atttttatca acaaaatgtt ctaagaattt agcagctgta
     3061 attgcgccgc cccatctgct gctgccaaga tttgcaacat ctgcaagttc acttttgatc
     3121 aactcattgt aatcatccca cataggtaag cgccacacac gatcgtaagt ggtcattcca
     3181 cttttataaa gattatcggc catcgcatca ttttttgtaa atacacctgc tacaaactca
     3241 ccgagtgcca caacacacgc accggttaag gtggcaagat caataatttg atccggtgtt
     3301 aactgtgaag cataatgcaa ggcatccgct aaaacaactc ttccttcagc atctgtatct
     3361 tctatctcaa ttgttttacc actggatgtt tttataatat cacccggacg aattgattca
     3421 cccgacacca tattttctgc agaaggtatg attccatata aatgaacagg caaactattt
     3481 tttgcggcag caattatagt tccgactact actgcagcac cggacatatc agctttcatt
     3541 tcgcccatat ttttggccgg tttaagtgag atgccgcctg tatcgaatgt tattcctttg
     3601 ccgaccagtg cgatagtttt aattttattt tttttattct tctttgattt tacagttggt
     3661 ttgtattcaa ggattataaa tcttggttta ttagtacttc cctgtcctat tgcaattaat
     3721 ccgcccattt ttcttttgcg gatttcatcc tcaccaaata cagtcgtttt gatgccaaaa
     3781 ggtttaactt cgctaacaac tcttttagca aaaagatcag gcgttaaatt attacttggt
     3841 tcgttttgta agtctcttgt aagattaaca gaagtaatta atttatcagc aacagaaata
     3901 gcatttttta atttcttttc attgtcagaa taaaagtaaa catcaagtac tttactctta
     3961 actttttttg atttgtattt atcaaaagaa taattgccga gtaaaacacc ttcagcgaaa
     4021 gtttgatagt aatattcttc agattcaaaa tattttttta ctgctgaata agccggaata
     4081 aatatgtgga gattttttgt cgcttcattc tttaaagtag aaataaactc tgccagatgg
     4141 tttctgaaat agtctgcatc aaatttatca tccagtttaa ctttggacag gattaatgca
     4201 tcaggttttc cggatggttt agtaacacga atttttccac cttcagctta taaaaaggtt
     4261 tttctctgaa aatcagaaaa ctttatgtta aagatctttt ccaaattagg tagactcttt
     4321 gctaattttt tatcatcaat aagatatttt agactaactg aaagtggatt ataaataatt
     4381 tttgatccgc ctgttttaag gtttatttta tacataaagt ctactttcat ttgttgagat
     4441 aaagttttgc ttttgaaaga attacaggta tcatttcctg catattattt gtaaaaaacc
     4501 tagcacctgc agcatttgta tgccc
//
LOCUS       DASWBY010000262         4324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_497395, whole genome shotgun sequence.
ACCESSION   DASWBY010000262 DASWBY010000000
VERSION     DASWBY010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497395"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VF870_04790"
     CDS             complement(<1..276)
                     /locus_tag="VF870_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251533.1"
                     /translation="MKLLVLITYLFIQTVVTQQIFIINAQDKHEIVKDCNYTFEEAVT
                     GIEIPESIINQLTLVDVEYYSFDEKLHRGQLVINKKATKDIIEIFQFI"
     gene            344..1168
                     /locus_tag="VF870_04795"
     CDS             344..1168
                     /locus_tag="VF870_04795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9251534.1"
                     /translation="MKSILIALFSVVFIFGCSNNPDSVTLKSGLKYNDIKVGTGIEAK
                     NGDLVEIHFKGWIVKDSSNLFSDWSVDSTKKADLIADSYAMNQPMKFVLGTESFIKGS
                     EEGIVGMKAGGQRASVIPSNLAYGPQGMGPIPPNTNIKVLIELVSAKEAVVAKMWDVD
                     STLFKTTASGLKYAIIQQGEGELVGKEKQVTVHYSGFLLDGTKFDSSVERDEPFTFVA
                     GVGQVIPGWDEGVQLMKKGSKARLIIPSNLAYGERDLGKIPPNSTLIFDVEVLDVK"
     gene            complement(1245..2111)
                     /locus_tag="VF870_04800"
     CDS             complement(1245..2111)
                     /locus_tag="VF870_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX9251535.1"
                     /translation="MKEITKQKIESIIDSEIISSKPVSGGCINNAEIIKTSSGNTFFV
                     KTNYDSEKDMFIKEADGLSELSKANAIRIPKVIYADYDFIIIENIIAAAKNKLFWEDF
                     GRNFAKLHKFTSNAFGFYEDNYIGSTSQINIAGEKESIDWTEFYFNKRLLFQFKLAEK
                     KGYAGDELRKYFSGLENKINIILKDCDVKPSLLHGDLWSGNFIIDDTGNSCLIDPAVY
                     YGHREADLAMTKLFGGFDSRFYSAYDEEYPLDNEYQYRENVYKLYHVLNHLNLFGSGY
                     YNQAVSLIKFYL"
     gene            complement(2083..2580)
                     /locus_tag="VF870_04805"
     CDS             complement(2083..2580)
                     /locus_tag="VF870_04805"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX9251536.1"
                     /translation="MVKVLFVCMGNICRSPAAEGIFKKLVNNANLGSLISIESAGTIG
                     YHTGELPDRRMRKRAQERGYNLDSRARQFNPKKDFEEFDYIITMDDDNYSDVVSLDNN
                     KSHSNKIFKMADFISDKNVREVPDPYYGGTEGFDFVIDILEDGTKNLLNKIKEDLERN
                     NKTEN"
     gene            complement(2727..3149)
                     /locus_tag="VF870_04810"
     CDS             complement(2727..3149)
                     /locus_tag="VF870_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX9251537.1"
                     /translation="MWIQKEITLKPRSKGFHLITDEILSYLPEIKAIKIGLLNILLKH
                     TSASITLNENADPSVRSDMKKYFDEIVPENKKYFEHISEGADDMPAHLKTSLLGNSIS
                     IPVTNGNLNLGIWQGIYLCEHRNHGGSRKIVLTMNGEN"
     gene            complement(3215..>4324)
                     /locus_tag="VF870_04815"
     CDS             complement(3215..>4324)
                     /locus_tag="VF870_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9251538.1"
                     /translation="VVSAKRESLNFIPLVELQNQIDSTLAPKSYLLESNIKASISTEV
                     IEVQGKSVNVGAYLDAANDKFKDEYMVLGAHFDHLGWGGQNSLYMGEPAIHNGADDNA
                     SGTSGLLELAEKFASIKNQLDRKIVFIAFSGEELGLLGSSYVVNNFPLPIENDITMIN
                     MDMIGRLNDKNDLIVYGTGTSSKWKNILDDKNEYDLNLTFNDEGFGPSDHSSFYGKKV
                     PVLFFFTGTHSDYHKPSDDTEKINAAGEEKVLKYVYDVALTIVNSDTRPDYISVERKD
                     TGKMTGTKVYVGTVPDFAGEVDGYKLGGVTDGSPAAKAGLQAGDIITQFGEKKISNIY
                     DFTYALGNYVPGDKVKVLVKRGEEQLTFEIELGSR"
BASE COUNT         1361 a          762 c          643 g         1558 t
ORIGIN      
        1 tatgaattga aatatctcga taatatcttt agtcgctttt ttatttataa ctagctggcc
       61 tctgtgcagt ttttcatcaa aagaataata ctcaacatcg accaaagtaa gttgattaat
      121 tattgattca ggtatctcaa ttcctgttac agcttcttca aaagtgtaat tacaatcctt
      181 aactatttca tgcttgtctt gagcattaat tatgaatatt tgttgggtta caactgtttg
      241 aataaataaa taagtaatta gcaccagtaa cttcataaca aaaattgtct atgttttatt
      301 tgaataattg aatatattcg tccctcaatt taaaggaaaa ttcatgaaaa gtattcttat
      361 tgcacttttt agtgtagtgt tcatttttgg ttgttcaaac aacccggatt cagttaccct
      421 caaatcagga cttaaataca atgatataaa ggttggaact ggtattgaag caaaaaatgg
      481 tgatctggta gaaattcatt ttaaaggttg gattgtaaaa gattcttcaa atctttttag
      541 tgactggtct gttgactcta ctaaaaaagc tgatctaatc gctgattctt atgcaatgaa
      601 tcagccgatg aaattcgttc ttggtacaga atcttttatt aaaggttctg aagaaggtat
      661 tgttggaatg aaagcgggtg gacaaagagc aagtgttatt ccatctaatc ttgcatatgg
      721 accacaaggt atgggtccga ttccgcctaa tacaaacatt aaagttttaa ttgaacttgt
      781 aagtgcaaaa gaggcagttg tggcaaaaat gtgggatgtt gattctactt tatttaaaac
      841 gacagcgtca ggtttaaagt atgcaatcat tcagcagggt gagggcgaac ttgttggtaa
      901 agaaaaacaa gttactgttc attacagcgg atttttatta gacggaacta agtttgatag
      961 ttctgttgaa cgtgatgaac cttttacatt tgttgctggt gtcggtcagg ttattcccgg
     1021 ttgggatgaa ggtgttcagt taatgaaaaa gggaagtaaa gcaagattaa taatcccatc
     1081 aaacttagcg tatggtgagc gtgatcttgg aaagattcca ccaaattcaa ctttaatttt
     1141 tgatgttgaa gttttagacg taaaataatt ctttaatcat ttcgaaattt tagcaaaaat
     1201 tttaatgcca ggctctttaa aacaagcctg gcatttttgt atttctaaag ataaaatttt
     1261 atcaaactga ctgcctgatt ataatatccg cttccaaata aatttaaatg atttagtacg
     1321 tgataaagtt tgtaaacatt ctcacgatat tgatattcat tatctaatgg atattcttca
     1381 tcgtatgcag aataaaacct gctgtcaaat cctccaaata attttgtcat tgccagatct
     1441 gcctcacgat gaccatagta aactgcagga tcaattaaac atgaattccc tgtatcatcg
     1501 attataaaat tcccactcca taaatcacca tgcagtaaac ttggttttac atcacaatcc
     1561 ttaagaataa tattaatttt attttccaaa ccggaaaaat atttacgtag ttcatcaccg
     1621 gcatagcctt ttttttctgc aagtttaaat tgaaataaca accgcttatt aaaataaaac
     1681 tcggtccaat caattgattc tttttcacct gcaatattaa tttgtgaagt tgaaccaata
     1741 taattgtctt cataaaagcc aaatgcatta cttgtaaact tgtgaagctt tgcaaaattt
     1801 cttccgaaat cttcccagaa taatttattt tttgcagcag ctattatgtt ctcaataatt
     1861 ataaaatcat aatcagcata aattactttt gggatacgaa ttgcatttgc cttcgatagc
     1921 tcactcaggc catcagcttc ttttatgaac atatcttttt cagaatcata atttgtttta
     1981 acaaaaaatg tattaccaga agatgttttt atgatctctg cattattaat acatccaccg
     2041 gaaaccggtt tacttgatat gatttcacta tcgataatcg attcaatttt ctgttttgtt
     2101 atttctttca agatcttctt ttattttatt taaaagattt tttgtcccgt cttctagaat
     2161 atcgatcaca aaatcgaaac cttcggtacc tccataataa ggatccggta cctctctgac
     2221 atttttatca gatataaaat ctgccatttt aaaaatttta ttactatgtg atttattatt
     2281 atcaagactt actacatccg aataattatc atcatccatc gtgattatat aatcaaactc
     2341 ctcaaaatct tttttaggat tgaattgtct ggcacggctg tccaaattgt agcctctttc
     2401 ttgtgcgcgc tttctcattc gtctatcagg caattctcca gtatgataac ctattgttcc
     2461 tgctgattcg atcgaaatca aacttcccaa atttgcgtta ttaactaact ttttaaatat
     2521 gccttcagca gcgggggagc ggcaaatatt tcccatgcat acaaataata ctttaaccat
     2581 tatttccttt aagatttaaa atcgatttta ggattaacac tatattgtct aattaacatt
     2641 tcaataaaat atatcattcg attaatactt tttaaatata gaattcaaag aagcagtata
     2701 gttagactta ctagttcttt acattattaa ttttcgccat tcatagtgag tacaattttt
     2761 ctgcttccgc cgtgattcct gtgctcgcaa agataaatgc cttgccatat acctaaattt
     2821 aaattcccat ttgtaacggg aattgaaatt gagttgccta acagagatgt ttttaaatgt
     2881 gcaggcatat catctgcacc ttctgatatg tgttcaaaat actttttatt ttcaggcact
     2941 atctcatcaa aatatttttt catatcgctt cttacactag ggtcagcatt ttcatttagc
     3001 gttattgaag ctgaggtatg ttttaataag atatttaata gtccgatttt gattgcttta
     3061 atttctggga ggtacgataa aatttcatcg gttatcagat gaaatccttt actgcggggc
     3121 tttaatgtaa tttctttttg tatccacata ataaaagtaa cgcggattaa ataacataac
     3181 gcagatttta aatctgcgtt atgaattaaa tattttatct ggatcccaac tcaatctcaa
     3241 atgttaattg ttcttcaccg cgctttacta aaacttttac tttatctcct ggaacataat
     3301 ttcctaatgc atatgtaaaa tcataaatgt tggaaatctt tttttcacca aattgtgtta
     3361 tgatatcgcc cgcttgtaaa cctgcttttg ctgcaggact tccatctgta accccgccaa
     3421 gtttatagcc atcaacttct ccagcaaaat cgggaacggt accaacataa acttttgtac
     3481 ccgtcatttt tcctgtatct tttctctcaa cagagatata atcaggtcgt gtatcagaat
     3541 taactatcgt taatgcaaca tcataaacat attttaatac tttttcctct ccagcagcat
     3601 taattttttc tgtatcatca gatggtttgt gataatctga gtgtgttcct gtaaagaaaa
     3661 ataataccgg tactttcttt ccataaaaag atgagtgatc gctaggtcca aatccttcat
     3721 cgttaaaagt taaatttaaa tcatattcat ttttatcatc cagaatattc ttccattttg
     3781 acgatgttcc tgttccataa acgatcaaat catttttatc atttaatctt cctatcatat
     3841 ccatatttat catagttata tcattctcaa tagggagtgg gaaattattt acaacataac
     3901 ttgatccaag tagtccaagt tcttcaccag agaacgcgat gaaaactatt tttctatcta
     3961 actgattttt tatcgatgcg aacttttcag ccaattccaa taaaccggat gtaccggaag
     4021 cattatcatc agctccgttg tggatagcag gttcacccat atacaaagaa ttttgaccgc
     4081 cccagccaag gtgatcaaag tgggcaccaa gaaccatgta ttcatcttta aatttatcat
     4141 tcgcagcatc aaggtaagcg ccaacattta cacttttacc ttgaacttca atcacttctg
     4201 tcgatatcga tgctttgata tttgattcta ataaatatga ttttggtgct agagttgaat
     4261 caatttgatt ctgtaattct accagtggaa taaaatttaa gctttctctt tttgcactaa
     4321 ctac
//
LOCUS       DASWBY010000263         5202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_196460, whole genome shotgun sequence.
ACCESSION   DASWBY010000263 DASWBY010000000
VERSION     DASWBY010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196460"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /gene="pnp"
                     /locus_tag="VF870_04820"
     CDS             <1..1723
                     /gene="pnp"
                     /locus_tag="VF870_04820"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX9251539.1"
                     /translation="AISDIPFDGPMAEVRVGRINGGLIVNPTHEQIKLGDIELVVAGT
                     ADSIMMVEGESKEVGEQEMLDALKFAQQEIKKIVELQNQLVAEAGKTKWIVEEKTVDA
                     NLKKDVYDLAEAKFKEIVYSILAKEERASKNKELSESVKQALAEKYPEQEKVIGGILH
                     DMEKDLMRERILKEGVRLDGRNTTQVRPITIELGNLPRTHGSALFTRGETQSLTNVTL
                     GTKDDEQTVDGLLEEYTKKFYLQYNFPPFSVGEVGRMTGVGRREIGHGNLAERSLKQV
                     LPADDVFPYTVRIISDILESNGSSSMATVCAGSLAMMDAGVPITKAVSGIAMGLVKEG
                     NDYAVLSDILGNEDHLGDMDFKVAGTSDGITGFQMDIKIQGISFEIMEKALHQAKEGR
                     MHILGKMNEAISKPKDTLSPYAPRLVTMNVATDQIGLIIGPGGKTIQGMQRLFGVDIN
                     IEDDGTIHIASPNKENAQQAKEYIKKLTATPEVGEVYDGVVTKIADFGAFVEILPGKE
                     GLLHISEIDNKRVNKVSDYLKVGDKVTVKLLKVEGGKFSLSRRKLLAEAAEKEAKNNP
                     PAETPAN"
     gene            2147..3706
                     /locus_tag="VF870_04825"
     CDS             2147..3706
                     /locus_tag="VF870_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX9251540.1"
                     /translation="MIKEIIINSSSNQTRVAITEDGNLADFFVDYPENRRMVGDIYLG
                     RVARVLPGIRAAFIDIGMKHDGFLHFSDIGDRTKQFQDMLGDEDSDVDLSDDDEAKPN
                     RTNGNSNPPLEPVITKLRKGEEILVQITKEPVSNKGVRVTSSVSLPGRFCVLLPFDNK
                     IGISKKISDYKERRRLRNIARGILPPNYGLIIRTVAKDQTEDAVKDDLTNLVKTWKTI
                     EATAKSEEPPSIVHQDLSTTDSVIRDLLTPDISKVFVDSKKLYKQIKGYVQLVQPGLL
                     EKIELYKSSSSIFDEFKIEEQIKTLMGRKVPLPSGGYLIIEHTEAMVVIDVNSGRYAK
                     SKEQELNSLKTDLEAAREIARQLRLRDIGGIIVIDFIDLEEDKNRKKVYDELKKEFRR
                     DRSKVSVLPMSDFGLVQITRQRIRQNIMQAMKDVCPVCGGSGFMTKESHLVYDIEDWL
                     KRFRRFSREFSLIIKCHPSDASRLQDKKFTKIQLKYLVRLRIEEDASIAMGKFRFFSK
                     KKGIDLTEKFK"
     gene            3723..4370
                     /gene="fsa"
                     /locus_tag="VF870_04830"
     CDS             3723..4370
                     /gene="fsa"
                     /locus_tag="VF870_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX9251541.1"
                     /translation="MKFFIDTANINEIKEAAALGILDGVTTNPSLVSKEGKDFRKLLD
                     EILALVDGPVSAEVISTDYEGILKEAHELSKIHKNIVVKVPLIKEGLKAVRTLSSENI
                     KTNVTLCFSASQALLAAKAGATYISPFVGRLDDISHDGMELISQIVQIYKNYNYKTEV
                     LVASIRHPLHLVESALMGADVCTMPFSVIDKLFNHPLTDIGLEKFLNDWKKTQSK"
     gene            4401..>5202
                     /gene="gcvT"
                     /locus_tag="VF870_04835"
     CDS             4401..>5202
                     /gene="gcvT"
                     /locus_tag="VF870_04835"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX9251542.1"
                     /translation="MKRTTFYNIHKNLGAKIVEFAGYEMPIQYSSIIAEHKAVRNSVG
                     VFDVSHMGEVFVKGDKALDFVQHITVNDASKLFPGRVQYSAMCYEDGGIVDDLLVYRI
                     AQNEFLLVINASNIDKDFAWMQKNNKFDVELINCSDEYSLLAVQGPNSLKTLQKLTDT
                     QINLEYYHFTQINLAGIDVIFSRTGYTGELGYELYFTGDEKVAEDLWDKIFEAGKEFD
                     IQPVGLAARDSLRLEMGFCLYGNDIDQTTNPLEAGLGWITKLSKSEFIA"
BASE COUNT         1775 a          804 c         1059 g         1564 t
ORIGIN      
        1 tgccatttca gatattcctt tcgatggccc gatggctgaa gttagagttg gaagaattaa
       61 tggtggactt atcgttaacc cgactcacga gcaaattaag ttaggtgata tcgaacttgt
      121 tgttgcgggc acagcagact ccattatgat ggttgaaggc gaatctaaag aggtgggcga
      181 acaggaaatg cttgatgcct taaaatttgc tcagcaggaa atcaaaaaaa ttgttgaact
      241 tcaaaatcaa ttagtagcag aagctggaaa aactaaatgg attgttgagg aaaaaaccgt
      301 tgatgcaaat cttaaaaaag atgtttatga tttggctgaa gcaaagttta aagaaatcgt
      361 ttattctatt cttgctaaag aagaaagagc ttcaaagaat aaagaacttt ctgaatctgt
      421 aaaacaagca cttgctgaaa aatatcctga gcaagaaaaa gttattggtg gaattcttca
      481 cgatatggaa aaagatctta tgcgtgaaag aattttaaag gaaggtgttc gccttgatgg
      541 aagaaatact acacaggtta gaccgataac aattgaactt ggtaatttac caagaacaca
      601 cggctcagca ctgtttactc gaggtgaaac acagagctta acaaatgtta cactcggtac
      661 aaaagacgat gagcagacag ttgatggatt gttggaagag tacacgaaaa aattctatct
      721 tcaatacaat tttccgccgt tcagtgttgg tgaagttgga agaatgacag gagttggaag
      781 aagagaaatc ggtcatggta atcttgcaga aagatcttta aaacaagttt tgcctgctga
      841 tgatgtattt ccatataccg ttagaattat ttccgacata cttgaatcga atggttcatc
      901 atcaatggca acagtatgtg caggttcgct tgcaatgatg gatgccggcg taccaattac
      961 taaagcagtt tccggtattg caatgggtct tgttaaagaa ggaaatgatt atgcagttct
     1021 ttctgatata cttggaaatg aagatcacct tggtgatatg gattttaagg ttgcaggaac
     1081 aagcgatggc attacaggat tccagatgga tattaaaatt cagggaatct ccttcgaaat
     1141 tatggaaaaa gctcttcatc aggcaaaaga aggaagaatg cacatacttg gtaagatgaa
     1201 tgaagcaata agtaaaccta aagatacatt atctccttat gcacctagat tagtaaccat
     1261 gaatgttgca acagaccaga tcggtttaat tatcggtcct ggtggaaaaa caattcaggg
     1321 tatgcaaaga ttatttggtg ttgatattaa tattgaggat gatggaacaa tacacattgc
     1381 atcgccaaat aaagaaaatg cgcagcaagc aaaagagtac atcaaaaaat taactgcaac
     1441 tcccgaagtt ggagaagttt atgatggtgt tgttacgaag attgcagact ttggtgcgtt
     1501 tgtagaaatt cttcctggta aggaagggct gcttcatatt tctgaaattg ataataaacg
     1561 tgttaataaa gtttctgatt atctgaaagt tggtgataag gtcacagtta agcttctaaa
     1621 agttgaaggc ggaaaatttt ctttaagtag aagaaaatta ttagctgaag ctgcggaaaa
     1681 agaagctaaa aataatccac cagcagagac accagctaat tagttatgta attattttat
     1741 aatgtcattt ctgtcgaagg cgttccttcg gaagaaggag ctttcgatga ctagaaatct
     1801 ttttaattat tgaaagaaga tttcccttgc tgattgaaat gactttacat actctgatat
     1861 tattttttta tgtcacactt ctacttcgct cagtgtgaca agttcgattt tgcagtcatc
     1921 ctgagcgaag ccgaaggatg acttgttgat atttaaataa atttttcaaa taggatttta
     1981 atgctattag cattaatata tttttttaga agtttcacaa ctcaacccct gctgttgaac
     2041 aacagccgag aaatttactg cactcagggc atacctttgc taaaattaga gttccaaaag
     2101 ttttcattaa tgaaatgttt tatttcatac aaagagtttt ttaatcatga taaaagaaat
     2161 tataataaat tcatcttcca accagacgcg cgtagcgatt acggaagatg gaaacttagc
     2221 cgactttttt gttgactatc ctgaaaacag gagaatggtt ggggacatat atcttggaag
     2281 agttgcacgt gtacttccgg gaataagagc cgcatttata gatataggaa tgaagcacga
     2341 tggcttcctt catttctcgg atattggtga tcgcaccaaa caattccagg atatgcttgg
     2401 cgatgaggat tcagatgttg atctttccga tgatgatgaa gctaaaccaa accgcacaaa
     2461 tggaaattca aacccaccgc ttgaaccagt gatcactaaa cttcgcaagg gcgaagaaat
     2521 tcttgttcag ataaccaagg aacctgttag taataaaggt gtacgtgtta cttcatctgt
     2581 ttcacttcct ggtagatttt gtgttctact tccatttgac aacaagattg gcatttcaaa
     2641 aaaaatttca gattacaaag aacgcagaag attgcgcaat attgctcgtg gtatccttcc
     2701 acccaattac ggcttgatta ttcgtactgt cgctaaagat caaactgaag acgcagttaa
     2761 agatgattta acaaatctcg tgaaaacatg gaagacaatt gaagcaactg caaagagcga
     2821 agaaccacca tcaattgtac atcaggattt aagtacaaca gatagtgtta ttcgtgattt
     2881 gttaacacct gatatttcta aagtttttgt tgattcaaaa aaactttaca aacaaataaa
     2941 aggatatgtt caattagttc aacctggttt gctggaaaaa attgagctgt acaaatcttc
     3001 gtcttcaata tttgatgaat ttaaaattga agaacagata aaaaccctaa tgggtagaaa
     3061 agttccgctg ccaagcggcg gttatcttat cattgaacat acagaagcaa tggttgttat
     3121 tgatgttaac agcggccggt atgcaaaaag taaagagcag gaacttaatt ctcttaaaac
     3181 agatcttgaa gcagctcgtg aaatcgcaag acaattacgc ctaagagata ttggcggaat
     3241 tatagttatt gattttattg atcttgaaga agataaaaac cgtaaaaaag tttatgatga
     3301 actgaaaaaa gaatttcgca gagatcgttc caaagtttct gttttaccaa tgagtgattt
     3361 tggtttggtg cagattacaa gacaaagaat ccgccagaat attatgcagg cgatgaaaga
     3421 tgtttgtccg gtttgcggcg gatcaggatt tatgactaaa gaatcgcacc tagtttatga
     3481 tattgaagac tggctgaaga gatttagaag attttctcgt gagttcagtt tgattataaa
     3541 atgtcatcca tctgatgcaa gcagattaca ggataaaaag tttaccaaaa tacaattaaa
     3601 atatttggtt agattgcgca ttgaagaaga tgccagcatt gcaatgggta agtttagatt
     3661 cttctctaag aaaaaaggga ttgatttaac agaaaagttt aaataatagt aggagtcttc
     3721 aaatgaaatt ttttattgat accgccaaca taaatgaaat caaagaagca gcagcacttg
     3781 gtatcctaga tggagttact acaaatcctt cgcttgtttc caaagaagga aaagatttta
     3841 gaaaattact ggatgaaatt ttagcattag ttgatggtcc ggttagcgct gaagttatct
     3901 ctactgatta tgaaggaata ttaaaggaag ctcacgagtt atctaaaatt cacaagaata
     3961 ttgtggttaa agttccttta attaaagaag gattaaaggc tgttcgcacc ctgtcttcag
     4021 aaaatattaa aacaaatgtt acactttgct tttcggcttc tcaggctttg cttgctgcaa
     4081 aagcaggcgc tacatacatt agtccttttg ttggaagact tgatgatata agccacgatg
     4141 gaatggaatt gatttctcaa atcgtgcaga tttacaaaaa ctacaactac aagacagaag
     4201 tacttgttgc aagcatcagg catcctttgc atcttgttga atctgcatta atgggcgcag
     4261 atgtttgtac aatgccattt tctgtaattg ataaattatt taatcatcca cttacagata
     4321 ttggtttaga aaaattctta aatgattgga aaaaaactca aagtaaataa attttagttc
     4381 aactattcgg aatagaaaaa atgaagcgca caacattcta taacattcat aaaaacttag
     4441 gtgcaaaaat tgttgagttt gccggttatg aaatgccaat tcaatattct tcaataattg
     4501 cggaacacaa agcggtaaga aattccgttg gtgtttttga tgtctcacat atgggcgaag
     4561 tttttgttaa aggagataag gctctggatt ttgttcagca cataacggtt aatgatgctt
     4621 cgaaactttt tccgggaaga gtgcagtact cggcaatgtg ttatgaagac ggaggcatag
     4681 ttgacgattt acttgtttac agaattgctc aaaacgaatt tttacttgta ataaatgcat
     4741 ccaacattga taaagatttt gcctggatgc aaaagaataa caaatttgat gtagagctta
     4801 taaattgtag tgatgagtat tctttgcttg ctgttcaggg gccaaattca cttaagactt
     4861 tgcaaaaact tacagataca caaatcaatc ttgagtatta ccattttacc caaattaatc
     4921 ttgccggtat cgatgtgata ttttcaagaa ccggttatac cggggaactt ggttatgaac
     4981 tctattttac cggcgatgaa aaagttgcag aagatttatg ggataaaata tttgaagctg
     5041 gaaaagagtt tgatattcaa ccggttggct tagccgcacg cgattcttta agattagaaa
     5101 tgggtttttg tctttatgga aatgatattg atcagaccac aaatccatta gaagcgggcc
     5161 tcggatggat aacaaaactt tctaaatctg aatttattgc ta
//
LOCUS       DASWBY010000264         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_84215, whole genome shotgun sequence.
ACCESSION   DASWBY010000264 DASWBY010000000
VERSION     DASWBY010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84215"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="VF870_04840"
     CDS             <1..1183
                     /locus_tag="VF870_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9251543.1"
                     /translation="SGYTIVEVRNDSIYFSERIPDTQTKLWYQLALQQRNYEALVTEQ
                     KRPDYSVNQKYPDVKLVWEFNSGFTIGSSAIVKDNSVFIGDASGYFYSLKLLDGSVNW
                     KYKTQNAIYSTPIIKCNLVVFGSADSSIYCLNNENGNLIWKYKTNAAVLGCPIIEDGM
                     VFIGSSDRKFRAIELETGKLVWEFSGLDGFVETRPAFYDDKILFGAWDEHFYCLDAKT
                     GKLLWKWKGDRDGIFYSPAACWPVVSNEIVFFAAPDRKLTAIDIETGNQLWRTGKYKV
                     RETVGLSEDREKLFIRTMNDTILALPVSDKLEEPLWITNCGFGYDISSAQIVEKDGVI
                     FYATKNGMIYSLDSETGKILWQHKSTNGFVNTITPISNTKIITTDFDGKVDLIKVDDT
                     E"
     gene            1515..3047
                     /locus_tag="VF870_04845"
     CDS             1515..3047
                     /locus_tag="VF870_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX9251544.1"
                     /translation="MTEKTGNANKYIEKFLGIIETIGNKLPNPTTLFALFALGVIILS
                     WIVSQFNFSVLHPGTGLEIKPVNLLSVEGLHRIILNLITNFTSFAPLGTVLVSLLGIA
                     VAEHSGLIGTVLRLLVLKSPKKLLTFVIVFAGILSNTASEIGYVLLVPLSAIIFLAAG
                     RHPIAGLAAAFAGVSGGYSANILLGTIDPLLAGLTQEAAHIIDKSYEVNAAANYYFMF
                     VSTFFIAAAGTWVTEKIVIPRLGAYQGDVKPEEIKPLSSDEKRGLWYSGIAMAIFGAI
                     IALGLIPENGFLRDPKTFSILKSPFLSGIVSFIFLGGVVAGLAYGIGAKTIKNDNDVI
                     KGMNKSMETLGSYIVLVFFAAQFVAFFNWTNLGMIVAVEGANLLKSSGLGPIPLLISF
                     IIISGFINLFIGSASAKWAIMAPVFIPMFMLLGYSPELVQGAYRVGDSVTNIISPMMS
                     YFALIIAFVGKYDSKTGIGTLIATMLPYTITFFIVWTILFIVWFLLGIPVGPGAQLFI
                     NN"
BASE COUNT          988 a          520 c          595 g         1065 t
ORIGIN      
        1 aagcggttac acaatagtag aagttaggaa tgattccatc tatttttctg aaagaatccc
       61 tgatacacaa actaaactct ggtatcaact tgctctgcag caaagaaatt atgaagcttt
      121 agtaacagag caaaagcgtc ctgattattc tgtcaatcaa aaatatccag atgttaaatt
      181 ggtttgggaa tttaattccg gctttactat tggatcttct gctattgtaa aagataattc
      241 tgtttttatt ggtgacgcgt ctggatattt ctattcatta aaattattgg atggatcagt
      301 taactggaaa tacaaaaccc agaatgctat ttactcaact ccgattatta aatgtaactt
      361 agtggtattt ggaagcgcag atagttcaat ctattgtttg aataatgaaa atggaaatct
      421 tatctggaaa tacaaaacaa acgctgcggt tctaggttgt cccattatag aagatggaat
      481 ggtatttatt ggaagcagcg atagaaaatt tagagcaata gaattggaaa caggaaaatt
      541 agtttgggag ttttcaggtt tggatggatt tgttgaaacc agacctgctt tttatgatga
      601 taaaatttta tttggtgcct gggatgaaca tttttattgc cttgatgcca aaacaggcaa
      661 acttctatgg aaatggaaag gtgatcggga tggaatattt tattcccctg ctgcttgctg
      721 gcctgttgtt tcaaatgaga tagtattctt tgcagcacct gatagaaaat taactgcaat
      781 tgatatcgag acaggaaatc agctttggcg aacaggaaaa tataaagtta gagagacagt
      841 tggactttca gaggatcgtg aaaaactttt tatccgaact atgaatgata caattcttgc
      901 cttaccggtt tccgataaac ttgaagaacc attatggatt acaaactgcg ggtttggata
      961 tgatattagc tcagcccaaa ttgttgagaa agatggagtt atattttatg caactaaaaa
     1021 cggaatgatt tattctcttg attctgaaac aggaaaaatc ctctggcagc ataaatcaac
     1081 caatggattt gtaaatacaa ttacgccaat aagtaatact aaaattatca caacagattt
     1141 tgatggaaaa gttgatctga taaaggttga tgatactgaa tgatcaataa tggaaacatg
     1201 gttaccgcca ctggcctgac cgcacatctg gaatggatga aacaatttta aaaattctgg
     1261 actcaaagat agaatcataa atttttattt ccatacttct ataattcctt caagatcaat
     1321 cattctgaac ttaaagtatg attaattatt aagcaataat tattataaac ctacaaaaaa
     1381 ctttagtttt ttaaactaat tctcctctaa tataattact cttttctgta gcatctgata
     1441 aaatattttt tttatattga actacaatcg ctagttgctt tgttacatct ctaaactaat
     1501 ccaaggataa cctaatgact gaaaaaacag ggaatgcaaa taagtatatc gaaaaatttt
     1561 tagggataat tgaaactatt ggaaataaac ttcctaatcc aaccactctt tttgctttgt
     1621 ttgcacttgg agtaattatt ctttcgtgga tcgttagcca atttaatttt tctgtgttgc
     1681 atccgggcac cggattagaa ataaaacccg taaatctcct ctctgttgaa gggcttcatc
     1741 gaattatctt aaaccttata accaacttta catcattcgc tcctctagga acggtattgg
     1801 tttcactttt gggaattgca gtagcagagc atagcggatt gattggaacg gttctccgtc
     1861 tgcttgtact caaatcacct aaaaaacttc taacctttgt tatagttttt gcaggaatac
     1921 tatcaaatac tgcaagtgag attggatatg ttttattagt gccactatca gctataattt
     1981 ttcttgcagc aggcagacat ccgattgcag gactagccgc agcttttgcc ggagtttccg
     2041 gaggatatag tgcaaatatt ttacttggta cgatagatcc tctgctggca gggcttacac
     2101 aggaagcagc acatattatt gataaatcgt atgaagttaa tgccgctgcg aattattact
     2161 ttatgtttgt atcaacattt tttattgccg ccgccggtac ctgggtaaca gaaaaaattg
     2221 ttattccaag acttggtgca taccagggtg atgttaaacc agaagagatc aaaccacttt
     2281 ccagcgatga aaaaagaggg ttatggtatt caggtattgc aatggcaata tttggtgcaa
     2341 tcatagcttt agggcttata ccagaaaatg gtttcttaag ggatccaaaa acattcagca
     2401 ttcttaaatc ccctttctta agcggaatcg tttcattcat atttcttggc ggtgttgttg
     2461 ccggtctggc ttatggtatc ggggcaaaga ctatcaaaaa tgataatgat gttattaaag
     2521 gtatgaataa atcgatggaa actttgggtt cttacatagt tcttgttttt tttgctgctc
     2581 agtttgttgc gttttttaac tggacaaacc ttggaatgat tgtagcggtt gaaggtgcaa
     2641 atcttttaaa atcttcaggg ctcgggccaa ttccactttt aatttcattc attattatct
     2701 caggatttat aaatttattt atcggtagcg cttctgccaa atgggcgata atggctcctg
     2761 tttttatacc aatgtttatg cttttgggat attcacctga gttagttcaa ggggcgtaca
     2821 gagtcggtga tagtgtaact aatataatct caccaatgat gtcttacttt gctttgataa
     2881 ttgctttcgt tggtaagtat gattctaaaa ccggaattgg aactcttatt gccacaatgc
     2941 tgccctacac aataacattc tttattgtct ggactatatt gtttatagta tggtttttat
     3001 taggaatacc tgtaggacca ggtgctcaat tgtttatcaa taactagaat aaaatattat
     3061 aatttctcat gcccctactg agaaggggct gagaaaactt ctttttactg cttacgaatc
     3121 tcaaacttct taggatcgta accctgatct tttaaaatct ggttaatc
//
LOCUS       DASWBY010000265         4841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_422667, whole genome shotgun sequence.
ACCESSION   DASWBY010000265 DASWBY010000000
VERSION     DASWBY010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422667"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VF870_04850"
     CDS             <1..634
                     /locus_tag="VF870_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9251545.1"
                     /translation="YNANAADEFKRNEIISGIKSLLNGKPLVKVIMHSLAFGTLKPFV
                     PSETEQAITKAQMEMTLDVMAHSLVYWVQDVVSNNLLADSARIFAMTSSGSHSAIPYY
                     GAVSAAKSALESHVRQLSVELGSRFGASVNSIMAGVTDTPALRKIPGNLDMIEIAQRK
                     NPHGRLTTPEDVAKVISLLCKDGGEWISGGLIHADGGEDIVNYVGQGKKS"
     gene            677..997
                     /gene="trxA"
                     /locus_tag="VF870_04855"
     CDS             677..997
                     /gene="trxA"
                     /locus_tag="VF870_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9251546.1"
                     /translation="MKPITFTNDNFEKEVLQSDKPVLVDFWAVWCGPCKIIAPYVEEL
                     AGELEGKAKVGKLDVDNNQESAIKYGVRSIPTVLIFKGGKVVDTIIGAVPKVQLKQKL
                     EAAI"
     gene            1296..4274
                     /locus_tag="VF870_04860"
     CDS             1296..4274
                     /locus_tag="VF870_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251547.1"
                     /translation="MIRKISVLFLALAFISVAFAQERGVGNEKRIVPDMNYVAPQAPT
                     STLEGTTAIGETFITTDYDYGGNNVIPKMISLADIDGTGDLDPFFVGMVRDNAAAIAR
                     YIAFGYSAFGAPIDAFRAFAPTQSLGWGTTQLCVGGALDGKALVMAHSGGVAWHSVID
                     LTNLAPVEPFPQTTFGSNFPDFVYQNDGTIWAATTDANIYKSTDVGATFSTVAPIGAG
                     DPNVTLVVANGPSENPLYGNSTGQYMATVGGWALMTVPDGNDGVYWYGTNDFGASWNG
                     LTIGVDGVYGQVANNPNLAPYFENFSQLNANVDETGTTHVVMNGYGEGINSVGDTVNT
                     FPIVYWNSNSGQWMEISLPSIAFEPNSIMATLRPGNGIGQAYPSVSVTDDGQFIFVIW
                     TAPEYTGTIGASPINIYNGTNQIFYTDLHWTTSTDGGQTWAAPQKIGEPNVSETYANV
                     ARRLEILPNGDVKAHFVFFVDAIPGTSLFAANNENALGTWQYISAIVGNYTPSTNIPV
                     TFQVDMGVQAFEGNFPAGANVVVRGDFQADAGDPGGNWQGNLFQLSDVDGDTVYTGTF
                     NMPASFAGTNYNFKYVIVNPPAGDNWESTPNRPFTLTAPATVNPVVWFNDDNVYTVIT
                     EVTNTINFTADISGILGVGVGGAFDPATDSLLVMGLDWDNLGKNVVGNRRMFNNDPFN
                     PGIYDASLTVTSGSAAANGVGDSTKWKFKAYPDGRFANTGWETTPDRWHIYQANGSTI
                     TLPTIVPSILPLFAPITSDVQITITVDMTGATNRYNGLTIPLNQLEFVGMRGAADFLG
                     SWATGGNWTPSDTTTGNMKVLTNIGGNLWRITVTAPAGQNAGQYAYKFAAMYPGALEV
                     NGGSSPLDNENPNDMNHTFILSDASPSVTLWNNWANITTDVKEIKDLVPVAFELGQNY
                     PNPFNPSTTIKFSVPEAGLVTLRVFNLLGQEVATLLNSEKTAGVYEATFDASTLSSGI
                     YFYTLEAKNFTSTKKMVLLK"
     gene            4402..>4841
                     /locus_tag="VF870_04865"
     CDS             4402..>4841
                     /locus_tag="VF870_04865"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9251548.1"
                     /translation="MKNSITKILFLFILVSLLFPALNAQTGVGKLSGKITDAATREAL
                     IGANVLLVGTSAGAASDVNGDYFILNITPGTYSVKVSYVGYAPKIIENVRIVAGITYE
                     LNVELTTDFTLDEIVVVDRKFFEEKSTNTVKVIDSDQISRLPVRG"
BASE COUNT         1541 a          831 c          965 g         1504 t
ORIGIN      
        1 ttataatgct aatgctgccg atgaatttaa gagaaatgaa ataatatcag gtataaaatc
       61 tctgttaaac ggaaaaccat tagtaaaagt tataatgcac tcacttgcat ttggaacttt
      121 aaaaccattt gtgccaagcg aaactgaaca ggcaattaca aaagcacaaa tggaaatgac
      181 tcttgatgta atggcgcact cacttgttta ttgggtgcag gatgttgttt caaataatct
      241 tcttgcagat agcgcaagaa tttttgcaat gacaagttca ggcagtcatt ccgcaattcc
      301 atactatggg gcagtatcag ctgctaaatc tgcattagaa tctcatgtaa gacaattatc
      361 agttgaactt ggaagtagat ttggtgcatc cgttaactca attatggcag gtgtaacaga
      421 tacaccggca ctccgcaaaa ttcctggcaa cttagatatg atcgaaatag cacaaagaaa
      481 aaatccgcat ggaagattaa ctactccgga agatgttgca aaagtaattt cactgctttg
      541 taaagatggc ggcgaatgga tttctggcgg cttgattcat gccgatggcg gtgaagatat
      601 agttaattat gttggacaag gaaaaaaatc ttaatcatct cataaaaaat tttatattaa
      661 tttttaagga tctgaaatga aaccaattac ttttactaac gataattttg aaaaagaagt
      721 tttgcaatct gataaacctg tgctggtaga tttttgggct gtatggtgcg ggccttgcaa
      781 aataattgct ccttatgttg aagaacttgc cggtgaatta gaaggcaaag caaaagttgg
      841 aaaacttgat gttgataaca atcaggaaag cgccataaaa tatggcgtaa gaagtatacc
      901 tacagtattg atttttaagg gtggaaaggt tgttgataca attatcggcg ccgttcctaa
      961 agttcagtta aagcaaaaat tagaagctgc tatttagtta gaggagaaga gaatttattt
     1021 ccaactaata agcccattta actaattaaa aatttaattg gttaaacccg gtttttagag
     1081 gttgaatatc atttttagag gaaaatagta tctcttgaca ttattaccaa aatatattat
     1141 ttcataagag caaataaaaa gactttagaa tcttgactaa aatcacaaac aattgtaatt
     1201 tggccgagaa ttttgatatt aattaaaata attaactgtt tagaatcaat tagaattaac
     1261 taaccacaaa acacttaaca caaaaggaga tcgttatgat cagaaaaatt tctgttttgt
     1321 ttctagcctt ggcatttatt agtgttgcct ttgctcagga acgtggtgtt ggtaatgaga
     1381 agagaatagt tcctgatatg aactatgtag ctcctcaagc tccaacttcg acccttgaag
     1441 gtactacagc tattggtgaa accttcatta caaccgatta tgattacggt ggtaataatg
     1501 ttatccctaa aatgatttca ctggctgata ttgacggcac aggcgattta gatccattct
     1561 ttgttggaat ggttagagac aacgcagccg caatagcaag atatattgct ttcggttata
     1621 gcgcttttgg tgcacctatt gatgcattta gagccttcgc accaactcag tcacttggtt
     1681 ggggaacaac tcaactttgc gttggtggag ctttagatgg taaggcatta gttatggcac
     1741 actcaggagg agttgcctgg cactcagtta ttgatttaac taacttagct cctgttgagc
     1801 ctttcccaca aactaccttt ggcagcaatt tccctgattt tgtttatcaa aatgacggaa
     1861 caatttgggc tgctacaaca gatgctaata tttataaatc tacagatgta ggtgcaacat
     1921 tttcaacagt cgctcccatt ggtgctggtg atccgaacgt tactttggta gtagctaatg
     1981 gtccatcaga aaacccactc tatggcaatt caaccggaca atatatggct acagttggtg
     2041 gctgggcatt aatgactgtc cctgatggta atgatggtgt ttattggtat ggtaccaacg
     2101 attttggcgc tagctggaat ggtttgacca tcggtgttga tggagtctat ggtcaggttg
     2161 caaataatcc aaacctagct ccttattttg aaaactttag tcagttaaat gctaacgttg
     2221 atgaaaccgg aacaactcac gttgtaatga atggttacgg cgaaggaata aattctgttg
     2281 gtgatactgt taatacattt ccaatcgttt attggaacag caattctggt caatggatgg
     2341 aaatttcttt accatcaatt gctttcgaac caaattctat aatggcaaca cttcgtccgg
     2401 gtaatggaat tggtcaagcc tatccatcag ttagtgttac tgatgatgga caatttatat
     2461 ttgtaatttg gacagctcct gaatatactg gtacaattgg tgcaagtcct ataaacattt
     2521 ataatggtac taatcaaatt ttctatacag atcttcattg gacaacatca acagatggtg
     2581 gacagacatg ggcagctcca caaaagattg gtgaaccaaa cgtttctgaa acatatgcaa
     2641 acgttgcaag aagattagaa atattaccaa atggcgatgt taaagctcat ttcgtatttt
     2701 ttgttgatgc tatcccgggt acttctctat tcgcagcaaa taatgaaaac gctttaggaa
     2761 cctggcagta tatttcagca attgttggta attatacacc tagcacaaat atccctgtaa
     2821 cattccaggt tgatatgggc gttcaggcat ttgaaggtaa tttccctgct ggtgcaaatg
     2881 tagttgttcg tggtgatttc caggcagatg ctggtgatcc aggcggaaac tggcaaggta
     2941 atttattcca attgtcagat gttgatggtg atacagttta cacaggtaca tttaatatgc
     3001 ctgcaagttt tgccggtact aattataact ttaaatatgt tattgttaat cctcctgcag
     3061 gcgacaattg ggaatcaacc ccaaacagac catttacact cacagctcct gcaactgtaa
     3121 atccagttgt ttggtttaat gatgacaatg tttatactgt aatcactgaa gtaacaaata
     3181 caataaactt cactgctgat ataagtggta ttcttggtgt aggtgttggc ggtgcattcg
     3241 atcctgcaac cgattcatta ttagttatgg gattagattg ggataactta ggtaaaaacg
     3301 ttgttggaaa cagaagaatg tttaacaacg atccatttaa tcctggaatt tatgatgcta
     3361 gcttaactgt tacatcaggt tctgctgctg ctaatggtgt tggagattct acaaaatgga
     3421 aattcaaagc atatcctgat ggaagatttg caaatacagg ctgggaaacc acacctgaca
     3481 gatggcacat atatcaggcc aatggctcta caattacttt accaactatc gttcctagta
     3541 tcttaccatt attcgcacct attactagtg atgttcaaat aacgattaca gttgacatga
     3601 ctggtgcaac aaacagatac aatggtctaa caattccatt aaatcaatta gagtttgttg
     3661 gtatgagagg cgctgcagat ttcttaggat catgggctac tggtggtaac tggactcctt
     3721 ctgatactac taccggaaat atgaaagtgc ttacaaatat tggcggcaat ttatggagaa
     3781 taacagtaac tgctccagca ggtcaaaatg caggtcagta tgcatacaaa tttgctgcaa
     3841 tgtatccagg tgctctagaa gttaacggtg gttcatctcc gcttgacaat gaaaatccta
     3901 atgatatgaa ccatacgttc atactttctg atgcttcccc aagtgttaca ctttggaata
     3961 attgggcaaa tatcactact gatgtaaaag aaataaaaga cctagttcca gttgcgtttg
     4021 agttaggaca aaattatcct aatccattca acccatcaac aacaatcaaa ttcagtgttc
     4081 ctgaagctgg attggttact ttaagagtat ttaacttgtt gggtcaggaa gtcgcgacat
     4141 tattaaacag cgaaaaaact gctggtgttt acgaagctac atttgatgct tcaacacttt
     4201 caagcggtat ttatttctat acattagaag caaagaactt tacctctacc aagaaaatgg
     4261 tattgttgaa gtaattatgg ttctttaatt aaaagcgggt ggttttctgt aaagacctta
     4321 cagtccatcc gcttttttta ttatatgatt aattcaatga gatgcaaatc aaatctctaa
     4381 aaacatatta gtggagaaga gatgaaaaat tcgattacaa agattttatt tttatttata
     4441 cttgtttctc tcctgtttcc tgcactgaat gctcagaccg gagtgggaaa attaagcggt
     4501 aaaattacag atgccgctac aagagaagca ctaataggcg ctaacgtcct gttagttggt
     4561 acttcggcag gtgctgcctc agatgtaaat ggtgactatt ttatacttaa tattacacct
     4621 ggcacatact ctgttaaagt tagctacgtt ggttatgctc ctaaaattat cgaaaatgtt
     4681 cgtatagttg caggaataac ctatgaatta aacgtagaac taactacaga ctttacacta
     4741 gatgaaattg tagttgttga cagaaaattc tttgaagaaa aatctacaaa tacagttaag
     4801 gttatagatt ccgatcaaat ttccagattg cctgttagag g
//
LOCUS       DASWBY010000266         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_610103, whole genome shotgun sequence.
ACCESSION   DASWBY010000266 DASWBY010000000
VERSION     DASWBY010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610103"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VF870_04870"
     CDS             complement(<1..822)
                     /locus_tag="VF870_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251549.1"
                     /translation="MIKILFLILFLSSSIISQQINFNSPENIKLFADYMFCDKDYLRA
                     IDEYEKYLLLVDDDSVEFKIGIAYSNINDQINACNRFILTKKSSPFYEQSKMEMLKSI
                     YLQNWDSSFYSTAELIINSQSIYSSNAYRLKNTSIFLSQNELPEKEKFLIPFKNDEKT
                     TLSGFYELKKNPPYKSEVLAGILSTLIPGSGKIYTKEYGDGITAFILTGLLGYLAYTN
                     FEHDHQTRAWIFTAIGAGFYAGNIYGSVVSAQIFNAKVNFDFDEGIKLYLEEKNYF"
     gene            complement(822..1262)
                     /gene="yidD"
                     /locus_tag="VF870_04875"
     CDS             complement(822..1262)
                     /gene="yidD"
                     /locus_tag="VF870_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX9251550.1"
                     /translation="MLLFFILLTFSVYPQTDWVRWEKSDPTYQIKNNSLEREYDLSIE
                     SVSDVIVKPVISAYWFFISDVDGDNCPFEPSCSSFFVKSVEETNILQGTIIFFDRFTK
                     DTNAFGRKEHYPKFGKNHFYDPVSLYTLDKAKINYIPTNTYLQE"
     gene            complement(1270..2415)
                     /locus_tag="VF870_04880"
     CDS             complement(1270..2415)
                     /locus_tag="VF870_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9251551.1"
                     /translation="MNFDLTEEHLMIQESAKDFALNEIAPSAVQRDKSAEFPFEIVKK
                     MGELGFMGMMVAPEYGGAGLDTISYVLAMIEISKVDASLGVIMSVNNSLVCYGLEKYG
                     TDFIKQKYLVPLAKGEKLGAFALSEPEAGSDATKQKTTADKDGDYFKLNGMKNWITNG
                     TSADYVLVMATTNKELAHKGISTFLVEKGTPGFDHGVKEDKLGIRSSDTCSLTFENCK
                     VPAENLIWEEGKGFNFAMNTLNGGRIGIASQAVGIAEASLEAAVKYAKERKAFGQTIS
                     NFQAIQFMLSDMAVKVDAAKLLTLKAAALKDAGKPYIKEGAMAKLYASKVAVQNALDA
                     IQIHGGYGYVREYLVERYLRDAKITEIYEGTSEIQKVVIARALLDNK"
     gene            2586..3701
                     /gene="rlmN"
                     /locus_tag="VF870_04885"
     CDS             2586..3701
                     /gene="rlmN"
                     /locus_tag="VF870_04885"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX9251552.1"
                     /translation="MAEQISTEESVSKTMLKGLTLNELKDFFTVAGEKRFRGDQVFEW
                     IYEHMAENFDDMSTIPNYLRKKLNLYCELNSLTLFSSEQSKRTGTKKYLFKTHDGLII
                     ESVLIPDPKRITLCISTQVGCPLDCQFCATGLMGYKRNLSSAEIIDQFLMVSRDYGKN
                     KISNIVYMGMGEPLLNYANTLKTLQIFAEGKTKEINLRRITVSTAGIAPKIIELADSG
                     IKVKLALSLHSMFEETRNKLMPINKKYSLAENLEAIRYYSKQTDTRITFEYVMLKDIN
                     DRTDDLNALIRLCKSISCKINVIPFNSISHMNPTGFAKELEPSQKFRVDEFVKKLREN
                     DIVVMVRYTQGDDIDAACGQLAYRTLNSVLSPSDQGD"
     gene            3709..4263
                     /locus_tag="VF870_04890"
     CDS             3709..4263
                     /locus_tag="VF870_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9251553.1"
                     /translation="MRKLTHDEISANRSTLESIHKVKKLPVIVVLNSIRSSYNVGSIF
                     RTSDGAMIEKLILCGYTPHPPIENKGNKDVLKTSLGSTESVKWEYIKNPVDAINELKS
                     KGFKICALELTENSKPYFDTTLYEFPLCLVVGNEITGVSQEVLNLCDYSIEIPQYGIK
                     QSLNVAVAYGIAIFELRKIFDENR"
BASE COUNT         1492 a          779 c          700 g         1455 t
ORIGIN      
        1 aaaatagttt ttttcttcta gatacaattt gattccttca tcaaaatcaa aatttacttt
       61 tgcattaaaa atttgtgctg atacaacaga accataaata tttcccgcat aaaaccctgc
      121 gccaatcgca gtaaatatcc atgcacgtgt ttgatgatca tgctcaaagt ttgtgtaggc
      181 tagatacccc aataaaccag ttaatataaa tgccgttatt ccgtccccat attcttttgt
      241 atatattttc cctgaaccgg gaataagtgt agaaaggatt cctgcaagta cttcactttt
      301 atagggtgga ttttttttta gttcataaaa accagacaaa gtggtcttct catcattctt
      361 aaaagggatt aaaaattttt ctttttcggg taattcattt tgagataaaa atattgaagt
      421 gttctttaat ctatatgcat tgctcgaata aattgattga gaatttataa tcaactcagc
      481 tgtggaataa aaacttgaat cccaattttg caagtagatt gatttgagca tttccatttt
      541 actctgttca taaaatggtg atgatttttt tgtaaggatg aatctattgc aggcatttat
      601 ctgatcgtta atatttgaat atgcaatacc tattttaaat tcaactgaat catcatcaac
      661 taacaataaa tatttttcat attcatcaat tgcacgcaga taatctttat cacagaacat
      721 ataatcggca aaaagtttta tgttttccgg tgaattaaaa ttaatctgtt gagaaatgat
      781 tgatgaactt aaaaaaagaa taaggaacaa tattttaatc attattcttg taagtaagta
      841 tttgtcggga tgtaatttat ctttgcttta tccagcgtgt ataaagatac cggatcataa
      901 aaatgattct tgccaaattt tggataatgt tcttttcttc caaaagcatt agtatctttc
      961 gtaaagcgat cgaaaaatat tattgtacct tgcagaatat tcgtttcttc aaccgattta
     1021 acaaaaaaac ttgaacacga tggctcaaaa ggacagttgt caccatctac atctgaaata
     1081 aaaaaccaat aagcactgat tacaggttta actattacat ctgatacaga ttctattgaa
     1141 agatcatatt ctctctctag agaattgttt tttatttgat aagttgggtc agatttttcc
     1201 catctgaccc aatctgtttg cggataaact gaaaaagtta acagaataaa aaataataac
     1261 aatatatgtt tacttattat caagtaaagc tctcgctatt acaactttct gaatttctga
     1321 agtaccttca taaatctctg taattttagc atcgcgtaaa tatctttcaa ccaggtattc
     1381 acgaacataa ccataaccgc catgaatttg aattgcatca agtgcatttt gaactgcaac
     1441 ttttgaagca taaagttttg ccattgcacc ttctttaata taaggtttcc cagcatcttt
     1501 aagtgctgca gcttttaatg tcagtaattt agctgcatca acttttacag ccatatctga
     1561 aagcataaat tgaatagcct gaaagttaga aatcgtttga ccgaatgctt ttctttcctt
     1621 agcatattta acagcagctt ctaatgatgc ttctgctata cccacagctt gagaagcgat
     1681 gccgattctt ccaccattta aagtgttcat tgcaaaatta aatcctttgc cttcttccca
     1741 tataagattt tctgcaggaa ctttgcagtt ctcaaaggtt aatgaacatg tgtctgaact
     1801 ccggattccg agtttatcct ctttaacacc gtgatcaaag cccggtgtac ctttttcaac
     1861 caaaaaggtt gagatgcctt tgtgagctaa ttctttgttt gttgttgcca tcactaaaac
     1921 ataatcggca gaagttccgt ttgtaatcca gtttttcatt ccatttaact taaaataatc
     1981 tccatcttta tcggcagtgg ttttttgttt tgttgcatca ctgccggctt ctggttctga
     2041 taatgcaaag gccccaagtt tttcaccttt tgctaatggt accaagtatt tctgcttaat
     2101 aaaatccgtt ccatattttt caagaccata acagaccaat gaattattaa ccgacattat
     2161 tacaccaaga cttgcatcaa ctttagaaat ctcgatcatt gcaaggacat aactgattgt
     2221 gtctaatcct gcaccgccat actcaggtgc aaccatcata cccataaaac caagttcgcc
     2281 catttttttt acgatctcaa aaggaaactc tgcagattta tctcgttgca cagcagaggg
     2341 tgcaatttca tttaatgcaa aatcctttgc ggattcttga atcattaaat gttcttcggt
     2401 gagatcaaaa ttcattttaa atcctttttt aatttgataa aaacctttta attttaaccc
     2461 gattgattta tgggtttaat ttatatcaaa aataatggaa taaaacttat gtatcaattc
     2521 gggggatgtt tgttaatatc cggaataaat attttttttg acaaatcata gtcgatggca
     2581 ttacaatggc agaacaaatt tcaactgagg aatcggttag caagacaatg cttaagggtt
     2641 taacccttaa tgaattaaaa gacttcttta ctgttgcagg ggaaaaaaga tttagaggtg
     2701 accaggtttt tgaatggatc tacgaacaca tggcagaaaa ttttgatgat atgtcaacca
     2761 ttccaaatta ccttagaaaa aaacttaatc tctactgcga acttaactcg cttactttgt
     2821 tctcttcaga acaatccaaa agaaccggaa caaaaaaata tttatttaaa acccacgatg
     2881 gattaatcat tgagtcggtt ttaatccccg atccaaaaag aattacgcta tgtatatcca
     2941 cacaagttgg atgtccgtta gattgccagt tttgtgcaac aggtttgatg ggatataaaa
     3001 gaaatctttc atctgcggag ataatcgatc aatttctgat ggtttccaga gattatggta
     3061 aaaacaagat ttctaatatc gtttatatgg ggatgggcga accattgctt aactatgcaa
     3121 atactttaaa gacacttcaa atttttgctg aaggaaaaac caaagaaatt aatttacgcc
     3181 gcataacggt ttcaactgct ggaattgcac caaaaattat tgaacttgca gacagcggaa
     3241 taaaagtaaa attggcacta tcacttcatt cgatgtttga agaaacccga aacaaattaa
     3301 tgcctatcaa caaaaaatat tcattggcag aaaatcttga agcaatcaga tattattcga
     3361 agcaaacaga cacacgaata acatttgaat atgtaatgtt aaaagatatt aacgatagga
     3421 cagacgatct taatgcgcta atcagattat gcaaatcaat ttcctgcaaa attaatgtta
     3481 taccattcaa ctcaatttca catatgaatc caacaggatt tgcaaaagaa cttgagccgt
     3541 cccaaaaatt ccgtgttgat gaatttgtta agaaactaag agaaaatgat atagtagtta
     3601 tggtaagata tactcagggt gatgatattg atgcggcctg tggacaatta gcatatagaa
     3661 ctctcaactc agtactctct ccttctgacc agggagatta agaacagaat gcgaaaactg
     3721 acccacgatg aaatttcagc aaatagaagc acactcgaat caattcataa agttaaaaaa
     3781 ctacctgtaa tagttgtatt aaactctatt cgcagctcgt acaatgttgg ttccatattc
     3841 cggacctcag atggcgcaat gatagagaag cttattttat gcggttacac accacatccg
     3901 ccaatcgaaa acaaagggaa caaagatgta ctaaaaacct cattgggttc tactgaaagt
     3961 gtcaagtggg agtatattaa aaatcccgtt gatgcaatta atgaattaaa atcaaaagga
     4021 tttaaaattt gtgcgcttga attaacagaa aacagcaaac cttatttcga tacaacctta
     4081 tatgaatttc cactttgcct tgtagtaggg aatgaaataa ccggagtgtc tcaggaagta
     4141 ttgaatttgt gtgattactc aattgaaatt ccgcaatacg gaattaagca atcgttaaat
     4201 gttgctgttg cttatggcat tgcaattttt gagttgagaa aaatattcga tgagaataga
     4261 tagaaattaa accgccgcag taaaaaactc ttctccatta tctttggtat gaatcatcaa
     4321 tagattagct cttaccattt tcactaaagt tcttgatgct cttctttcag aaatgttaac
     4381 aagggtactt aattctttca cacttattcg ttcaactttt gataaa
//
LOCUS       DASWBY010000267         4303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_124951, whole genome shotgun sequence.
ACCESSION   DASWBY010000267 DASWBY010000000
VERSION     DASWBY010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124951"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VF870_04895"
     CDS             complement(<1..309)
                     /locus_tag="VF870_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251554.1"
                     /translation="MFELTNTNSDMLSFFVSLGAKTELINGVTHIQSYTSLEDEVYSL
                     NNGVALKFMNTSSIIELKGTDSLDFLHRISTNSMKNLNKEEVKKTIFTSEKGRIIGVS
                     T"
     gene            complement(386..1216)
                     /locus_tag="VF870_04900"
     CDS             complement(386..1216)
                     /locus_tag="VF870_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251555.1"
                     /translation="MILLLLLSCEHKTKTEDSRINYNEQAAFDKNKDAEIQRYNLNLK
                     KKESKNRFPCDTIAVIEHIFDNYPPGTYLLEFDRTTTYNIPNPAIIYFTDKDGSRFVF
                     TVIATSRPGERLIEPANVIGYDESFIDLDSTKLGTPFLYLVLFECSGENLIKQWEAPI
                     PSHGGFNSISLKKWNYNSTYYLENNFHYAQGVGHINYNYFLIDNFRNYPHLLMTYSGT
                     SFKRTLANVNNDKFPDYYEHIFYNLNDRVYSKDSVAFIWSAKDTLYINTKNPKQTRRY
                     "
     gene            complement(1381..2745)
                     /locus_tag="VF870_04905"
     CDS             complement(1381..2745)
                     /locus_tag="VF870_04905"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX9251556.1"
                     /translation="MKEVIDYIENNRGRYIEELKDFLRIPSISTLAENKPDMQTAAKF
                     VADKLGDAGLNKVEIFQTEGHPLIYGEWLGAPGKPTVLIYGHYDVQPVDPIELWSSQP
                     FEPTIIGDNIFARGATDDKGQMYMHIKSVEAYFKTHGSLPLNVKFLVEGEEEIGSTNL
                     SVFLKNNTELLKCDAVLISDTALFAPGVPTLTYGLRGLCYMEIEVTGPSRDLHSGTFG
                     GGVPNPINVLTKMISQLIDKDGKIKITGFYDDVLKLSKKERENFIVLKFSEKEWGKAI
                     GLKHLSGEKGYTTLERIWARPTLDCNGIWGGFTGQGAKTVIPSKASAKISMRLVPHQD
                     PKKIEKLFTKYIQKIAPKEVSVKVTSLHGGSPVITLLDDKATIAGAKAMQLAFGKKTV
                     FMREGGSIPIVVEFADRLKASPVLMGLGLNSENLHSPNEHFNLKHYQLGIKSSAYFLD
                     EYSK"
     gene            complement(2757..3767)
                     /locus_tag="VF870_04910"
     CDS             complement(2757..3767)
                     /locus_tag="VF870_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251557.1"
                     /translation="MARILILFFIIYSNLFVFAQNQNTTLLPDDKFPLWLKSNQSRTD
                     QTSGIAFIKSGCGKKYFLLADDIGSIHLVSIKSDSIYSIDSISFINNTDKYFDSFPKK
                     DFEEIVYDKSTGFVYLSVEGNGKDFNEFVGIYKLTFNDNDILSKQVISIEKMIIKPAD
                     IFYKYTGWNIGYEGFALDNNYFYLGLEGFVNNSQFADSTLIFIANKSHLNIIKTISTK
                     SLGIYTICGLFSDEDYSLWGVDRNNRTIFHILFDTEFNIKNFSKYEGVTFIPGYHNLN
                     YLPSYESITMDDDDNIYIVDDPWKEVYIPQKDILDKLDNQTIKNFKKYIPIIFKYKII
                     NP"
     gene            3885..>4303
                     /locus_tag="VF870_04915"
     CDS             3885..>4303
                     /locus_tag="VF870_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX9251558.1"
                     /translation="MILFRYILKAHFIPFVFSLITLMCVFLLQFLMKFADRLVGKGLD
                     TWVITKLIAYNLSWMVVLVVPMASLVATLMAFGNLSQNNEVTIMKSSGISLYKMMFPP
                     FLASIVVAILLFQFNNDVLPDANHQAKILMSDISRTKP"
BASE COUNT         1385 a          738 c          659 g         1521 t
ORIGIN      
        1 ggtagaaaca ccaattattc ttcctttttc tgatgtaaaa attgttttct ttacttcttc
       61 tttattaaga tttttcattg aatttgtaga aatcctgtgt agaaaatcaa gagaatcagt
      121 tccttttaat tcaataatag aagaagtatt cataaacttt aacgcaaccc cattattaag
      181 gctataaact tcatcctcaa gtgaagtata actttgtata tgagttacac cattaatcaa
      241 ctcggtttta gccccaaggg aaacgaagaa tgataacata tctgaatttg tattagtcaa
      301 ctcgaacatt attactccat tgtatatctt ttgttttttc ggataagaaa gaggaaaaat
      361 tccgtttgca aagataagaa aaaaattaat aacgtcgagt ttgttttggg ttcttagtgt
      421 tgatgtatag tgtatctttc gcactccata tgaatgctac agaatcctta gaatagactc
      481 tatcgttaag attgtagaag atatgttcgt agtaatcagg aaatttatca ttattaacat
      541 ttgccagagt tcttttgaag ctggtaccgg agtatgtcat caacaaatgg gggtaattac
      601 ggaaattatc gatcaaaaag taattataat ttatatgccc aacaccctgc gcgtaatgaa
      661 aattgttttc cagataatat gtactgttat aattccattt ttttagcgag atgctgttaa
      721 aaccgccatg agatgggatt ggcgcttccc attgttttat taggttttca ccgctgcatt
      781 caaataaaac aagatataaa aaaggcgtac cgagttttgt actatctaaa tcaataaaac
      841 tctcatcata gccgattacg tttgccggtt cgatgagccg ttctccaggt cttgaggttg
      901 caattacagt aaacacaaat ctgcttccgt ctttatcagt aaaataaatt attgctggat
      961 ttggaatatt gtaagtggta gttctgtcaa attctaataa gtatgtaccc gggggataat
     1021 tatcaaagat gtgttcaatt actgcgattg tgtcacaagg aaatctgttt ttagattctt
     1081 ttttttttaa gttaagattg taacgttgaa tttcagcatc tttgttttta tcaaacgctg
     1141 cctgctcatt gtaatttatc ctactatctt ccgtttttgt tttatgctca cagcttaaca
     1201 gcagtaacaa aatcaaaaga ataattggat aaattagtga cgatagttta attttcttca
     1261 acatagatgc acaaataata caatatttat gcccctgcaa gaatcaaaaa aaagcctcag
     1321 aatttcatta aatcaaactc tgaggctgaa aaaaattaga ttaatcttat aataaaacag
     1381 ctatttagaa tactcatcca gaaaataagc tgagcttttt attcccaatt gataatgctt
     1441 aagattaaaa tgttcatttg gtgaatgtag attctccgaa ttcaatccca aacccatcaa
     1501 tactggtgaa gcttttaatc tgtctgcaaa ttcaactaca atcggaatag atccgccttc
     1561 acgcataaaa acagttttct ttccaaaggc taactgcatt gcttttgctc ctgcaatcgt
     1621 tgctttgtca tccaaaagtg taattacagg tgaaccacca tgtaaactag ttacttttac
     1681 gctaacttct tttggtgcaa ttttttgaat gtattttgta aaaagttttt caatcttttt
     1741 tgggtcctga tgagggacaa ggcgcatact aatttttgca gaagcttttg atggtattac
     1801 agttttagct ccctgtccgg taaatccacc ccaaattcca ttacaatcca gtgttggtct
     1861 tgcccagatt cgctccaaag ttgtatatcc tttttcaccg gaaagatgtt ttaatccgat
     1921 agctttgccc cactcttttt cagaaaattt aagtactata aaattttctc tttctttttt
     1981 ggataacttt aaaacatcgt cataaaaccc tgtaatttta attttgccgt ctttatcaat
     2041 taattgagaa atcatttttg taagcacatt tattgggttg ggaactccgc cgccaaatgt
     2101 accggagtga agatcacgtg atgggcctgt aacttctatt tccatataac ataatccgcg
     2161 caatccataa gttaatgttg gaacgccggg agcaaataaa gctgtatcgg aaattaagac
     2221 tgcatcacat tttagaagtt cggtattatt tttaagaaat actgataagt ttgtacttcc
     2281 aatttcttct tcaccctcaa ccaaaaattt tacgttaagc ggaagactac cgtgagtttt
     2341 aaagtatgct tcaacacttt tgatgtgcat atacatctga cctttatcat ccgtggcacc
     2401 gcgcgcaaaa atattatcac caataatcgt tggttcaaat ggctgtgatg accatagttc
     2461 gataggatcg acaggctgaa catcataatg cccataaatc aaaactgttg gttttcccgg
     2521 cgctccaagc cactcaccat aaattaatgg atgcccttca gtctggaaaa tttcaacttt
     2581 atttaaacct gcatcaccta acttatcggc aacaaacttt gctgctgtct gcatatcagg
     2641 tttattttca gccaacgtac ttatactggg aattcttaaa aaatctttta gctcttcaat
     2701 atatctgccc ctgttgttct caatgtaatc aattacttct ttcaattatg ttctccttaa
     2761 gggtttataa ttttatactt aaatattatt ggaatatatt ttttaaagtt ttttatagtc
     2821 tgattatcaa gtttatctaa aatatccttt tggggaatat agacttcttt ccacggatca
     2881 tcaacaatat agatattatc atcatcatcc atcgtaatac tttcgtaaga aggtaaatag
     2941 ttaagattat gataaccggg aataaaagtt acgccctcat atttagaaaa gttttttatg
     3001 ttaaattctg tatcaaaaag gatatgaaat atagttctat tgttcctgtc aactccccat
     3061 agtgaataat cttcatcaga aaataaacca catattgtat aaatgcctaa actctttgta
     3121 ctaattgttt taattatatt caaatgtgat ttatttgcaa taaaaattaa tgtgctgtct
     3181 gcaaactggg aattattaac aaatccttct agtcctaaat aaaaataatt attatctaat
     3241 gcaaaccctt cataaccaat attccaaccc gtgtacttat aaaaaatatc agccggttta
     3301 ataatcatct tttcaatcga aataacctgt ttggaaagta tatcattatc attaaaggtc
     3361 agcttataaa tacctacgaa ttcattgaag tcttttccat tgccttctac tgataaatat
     3421 acaaatcctg tgcttttatc ataaacaatt tcttcaaaat cctttttcgg aaaagaatca
     3481 aagtatttat cagtattgtt tataaaggaa atactatcaa tgctataaat tgagtcgctt
     3541 ttaattgaaa ccaaatgaat ggaaccgata tcatctgcaa gcaaaaaata ttttttaccg
     3601 catccgcttt tgataaatgc tattcctgaa gtttgatcgg ttcttgattg attacttttt
     3661 aaccagagtg gaaatttatc gtccgggagt aaagttgtat tttgattttg tgcaaataca
     3721 aagaggttgg aataaattat aaaaaataat attagtattc gcgccatcgt atagaattta
     3781 aaaatgaaat aataatttgc acaccaaata taagtataaa ccagatatca caaggtaaaa
     3841 ttgagtttct tgttaaactt ttaggtgtta gctatctttg attaatgatt ttattccgat
     3901 acatattaaa agctcatttc attccttttg tattttcatt aataacactg atgtgcgttt
     3961 ttctgcttca gtttttaatg aaatttgctg ataggttagt tggaaaagga ttagatacct
     4021 gggtgataac caaactaatc gcttacaatc tttcctggat ggtggttctg gttgtaccga
     4081 tggcttcatt agttgccaca ttaatggcat tcggaaatct tagccagaac aatgaagtaa
     4141 caatcatgaa atcttcagga attagtcttt ataaaatgat gtttccgcct tttcttgcaa
     4201 gtatagttgt tgcgattctt ctatttcagt ttaataatga tgttcttcct gatgcaaatc
     4261 atcaggcaaa aatattaatg agtgatattt cgcgaaccaa acc
//
LOCUS       DASWBY010000268         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_84345, whole genome shotgun sequence.
ACCESSION   DASWBY010000268 DASWBY010000000
VERSION     DASWBY010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84345"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VF870_04920"
     CDS             complement(<1..366)
                     /locus_tag="VF870_04920"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEX9251559.1"
                     /translation="MKKIISLSALSIFLFLCIGCAEKPKPELTEQIKNGLRQDAKEFM
                     DSLKSVLVKEMQTNGIVAAVSVCSDTAQLLTVNYGFSKGIYIKRVSLKYRNPNNAPDG
                     FESKVLNHFLQLSVEGKLDG"
     gene            476..1786
                     /locus_tag="VF870_04925"
     CDS             476..1786
                     /locus_tag="VF870_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251560.1"
                     /translation="MSTKRELTQKDIFSFWIPLAATWLMMSVEGPYLSALIARLAEPK
                     FNLAAYGIAFSLALIIEAPVIMMMSASTALVKDYHSFKQLRKFNYILSLSLTAAMIVF
                     ILPPIFYFVTEDLIGLPREVSYLTHLAVMILIPWPGAIGYRRFYQGILIRNNLTRLVA
                     YGTVIRLTSMSLTAFILFMFTDVPGVVVGASALSTAVICEAIASKLMVKKFLVKLKTH
                     VSEFQNELTMKEIIRFYYPLALTSILTLGIQPFVTFFIGQSRMAIESFAVMPVVTSFV
                     FIFRGLGLSYQEVVVALIGDNMGNFNPLKRFALKLASFVAGILMIIAFSPLAEIWFRD
                     VSGLSESLTDFAKLPLMIMSFFPAFTVLISFQRAILVKANDTKQITYGTAIEFVGIIL
                     VVAVCIKFFSLVGAVAATIAFVIGRIAACSYLTPPSIRALKIKD"
     gene            1834..2373
                     /gene="msrB"
                     /locus_tag="VF870_04930"
     CDS             1834..2373
                     /gene="msrB"
                     /locus_tag="VF870_04930"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX9251561.1"
                     /translation="MMRFMLIGVVLIAGIIFWNYSDNFTKGKNTQSKPFISDSTKKQG
                     AQMDDKIIKTDEEWKKELTPEQYRILREKGTERAFTGEYWDNHELGTYICAACGTELF
                     ESNTKFDSGCGWPSYFEPIDSSRIIYKEDKSFGMTRTEVMCAKCGGHLGHVFDDGPPP
                     TGLRYCINSGAMKFIKKEK"
     gene            2438..>3655
                     /locus_tag="VF870_04935"
     CDS             2438..>3655
                     /locus_tag="VF870_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9251562.1"
                     /translation="MSAANTNENQSVVNRSGWKFPRAFWTANIVELFERAAYYGVFIA
                     ITLYLSRIVGFDDIEAAWIGGIFSAGLYFLPPFTGALADKIGFRNAIILAFALLSLGY
                     FSLGALPYKAVVLPSLVVLMFGGSFIKSIITGTVAKTTTPETRAKGYSIFYGIVNIGA
                     FLGKTFAYPLRIEWGLTSINFFSALMSFIALIVVILFYKNVSADGEGKSVKEVWTGFV
                     KVVTNMRLLTLIFIVTGFWIIQHQLYATMPKYVLRTVGEQASPEWIANVNPAVVMALV
                     MFITHLMRNVKAVSSMAVGMFLMPVSALLMASSNLLESVTGQSVSFGGLFSAHPITVM
                     MIAGIMVQGLAECFISPRYLEYFSLQAPKGEEGLYLGFSHLHSFLSSILGFGISGYLL
                     TAYAPDPATLTPTQ"
BASE COUNT         1107 a          623 c          695 g         1230 t
ORIGIN      
        1 cccatcaagc tttccctcaa cgcttaattg taaaaaatga ttgagaactt ttgattcaaa
       61 tccatcagga gcattgttgg gatttcgata ctttaaagaa acgcgcttaa tatatattcc
      121 tttactaaaa ccatagttta ccgttagaag ctgagcggta tcagagcaaa cagaaacagc
      181 agccacaatt ccatttgttt gcatctcctt gactaaaaca gattttaaag aatccataaa
      241 ttcttttgca tcttgtctta atccattttt gatttgttcg gttagctctg gtttgggttt
      301 ttctgcacat ccaatgcaga gaaaaagaaa gattgataat gctgataatg aaatgatttt
      361 tttcataatt ctctcctgta ataaaaatta tcaattcccg ggattaattt aacaataaat
      421 atctaacgtt agacaaataa taattatgtt tgagcattaa atctgattgg atgttttgag
      481 tacaaagcgc gaacttacac aaaaagatat tttttcattc tggattccct tagccgcaac
      541 ctggttaatg atgtcggttg aaggtccata tctttctgca ttaattgcca gattagcgga
      601 acccaaattt aatctggcgg catatggaat cgcgttttca cttgccctga taattgaagc
      661 ccccgttata atgatgatga gtgcatcaac agcacttgta aaagattacc attcttttaa
      721 gcagttaaga aaatttaatt acatattaag tttatccctt actgcagcta tgattgtttt
      781 tattctgccg ccgatttttt atttcgttac agaagattta atcggtttgc cgagagaagt
      841 atcctactta acacatcttg ctgtaatgat tttaattccc tggcccggtg cgattggata
      901 ccgaagattt tatcagggga ttttaatccg taataatcta acgcgtttag tagcctatgg
      961 aactgttatc agattaactt ctatgagttt aaccgctttt atcctgttta tgtttacaga
     1021 tgttccggga gttgtagttg gcgcaagcgc attatccacc gcggttatat gtgaggctat
     1081 tgcaagtaaa ttgatggtca aaaaatttct tgtgaaatta aaaactcacg taagtgaatt
     1141 ccaaaatgaa ttaactatga aagaaattat aagattttat tatccacttg cacttacatc
     1201 gatactaacg ctcggcatcc agccatttgt aacttttttt attggacaaa gcaggatggc
     1261 aattgaatcc tttgctgtaa tgccagtagt tacatcattt gtttttattt tcagaggatt
     1321 aggcttgtca tatcaggaag ttgttgttgc tttgatcggg gataatatgg gtaatttcaa
     1381 tccgctaaaa agatttgcac ttaaactagc atcgtttgtt gccggcattt tgatgataat
     1441 agcatttagt cctcttgcag agatctggtt tagagatgta tcaggcttat cagaatcact
     1501 tactgatttt gcaaaacttc cattaatgat tatgtctttc tttcccgcct ttacagtact
     1561 aataagtttt caaagagcta ttttagttaa agccaacgat acaaaacaaa taacatatgg
     1621 aaccgcaatt gagtttgttg gaataatatt ggttgtagct gtttgtataa aatttttctc
     1681 ccttgtagga gctgtagctg caacaattgc atttgtgata ggacgaatag ctgcttgcag
     1741 ttatttaact ccaccctcca ttcgtgcatt gaaaatcaaa gattaatttt tcattataat
     1801 atcagaaaat tgaaaaaaaa ccaggaaaac attatgatga gatttatgtt aatcggtgta
     1861 gttttaatag cgggaattat attctggaat tattctgata attttaccaa gggaaagaac
     1921 actcagtcta agccttttat ttctgattca acaaaaaaac aaggagcgca aatggatgat
     1981 aaaattataa agacagatga agaatggaaa aaagaactaa caccagaaca gtatagaatt
     2041 ctgcgcgaga aaggaaccga aagagcattt accggggaat attgggataa tcatgaactt
     2101 ggaacctata tttgtgcagc ctgcggaact gaactttttg aatcgaatac taagtttgat
     2161 tccggctgcg gctggccaag ttactttgag cctattgaca gcagcagaat catctacaaa
     2221 gaggataaaa gttttggaat gacaaggacc gaagttatgt gcgcaaaatg cggcggccat
     2281 cttggtcacg tttttgatga cggccctcca ccaaccggtt tgagatactg cataaactcc
     2341 ggggcaatga agttcataaa aaaagaaaaa tagttgccta aaaagtttaa aatcatatct
     2401 tacagcttca atattcaaaa ttattaagag gtacactttg agtgcagcaa atacaaatga
     2461 aaatcaaagc gttgtaaata gatcaggctg gaaatttccc agagcatttt ggactgcaaa
     2521 catagttgag ctttttgaaa gagctgccta ttatggcgtg tttattgcaa ttacacttta
     2581 tctttcgcgt attgtaggct ttgatgatat tgaagctgca tggattggcg gcattttttc
     2641 tgcgggatta tattttctcc ctccctttac aggggcgctt gcggataaaa ttggtttcag
     2701 aaatgctatc attcttgcct ttgcgcttct atcgttagga tacttttctc ttggtgcact
     2761 tccgtataaa gccgttgttc ttccatcgct ggttgtttta atgttcggtg gatcatttat
     2821 aaaatctatt attacgggta cagttgcaaa aacaaccaca ccggaaacaa gagcaaaagg
     2881 ctattcaata ttttatggta ttgtaaacat tggggctttt ctcggtaaaa cttttgctta
     2941 tccattaaga attgaatggg gattaacttc tattaatttt ttctctgctt taatgagttt
     3001 tatagcatta attgttgtta ttctatttta taaaaatgtc agtgcagatg gagaaggaaa
     3061 atctgtaaaa gaagtatgga ccggatttgt aaaagtagta acaaatatgc gcttacttac
     3121 tttgattttt atcgttacag gattttggat aattcagcat cagctttatg caactatgcc
     3181 taagtatgtt ttaagaactg taggtgagca ggcttcacca gaatggattg ctaatgttaa
     3241 tcctgctgtt gtgatggcat tggttatgtt cataacccac ttaatgagaa atgttaaagc
     3301 cgtttcttca atggccgtgg gaatgttttt aatgcctgtc tcagcacttc ttatggcatc
     3361 aagcaattta ctggaatctg ttacaggtca atctgtttct tttggtggat tgttcagtgc
     3421 acatccgatt accgtaatga tgatagccgg gattatggta cagggtttag cggagtgctt
     3481 tatttcaccg cgttacttag aatacttctc ccttcaggca ccaaaaggtg aagaaggact
     3541 gtatcttggt tttagtcatc ttcattcatt tctttcatca atattaggat ttggaatctc
     3601 aggatattta ttaacagcct acgcacctga tcctgctact ttaactccaa cacag
//
LOCUS       DASWBY010000269         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_688845, whole genome shotgun sequence.
ACCESSION   DASWBY010000269 DASWBY010000000
VERSION     DASWBY010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688845"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF870_04940"
     CDS             <1..198
                     /locus_tag="VF870_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX9251563.1"
                     /translation="LDDSYINDFEKAVDGMTVEKANEIIKKYFPKNNLQFVLIGKADD
                     IREIAKKYGKVIEKNITDDGF"
     gene            complement(256..858)
                     /locus_tag="VF870_04945"
     CDS             complement(256..858)
                     /locus_tag="VF870_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251564.1"
                     /translation="MKHIIPLLIVLVLQSAYSQQKSDFILTPCIGNSILNSKTSEADL
                     VRIVGKKNVERVERWYAEGTERVVGSVFFKDTPQSFFIKWKDTVNFKNPDFIEIHGDK
                     SLWELDNGIKIGTELKELVRLNGKHFTFSGFDWDYGGYAVFEKGNLESDCYSIQLYYD
                     YKDLFENEWNQIVGDKTVSTKNPILSKIKIYVDEITFYFK"
     gene            complement(920..1390)
                     /locus_tag="VF870_04950"
     CDS             complement(920..1390)
                     /locus_tag="VF870_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9251565.1"
                     /translation="MKKIFLAFAFFLIAAISLSAQVSDSTLKFDPARNPFDDLKIAVD
                     SATKLNKRIILDVGGEWCIWCHRIDAFMHNTAEVKSLLEENFIVVKINYSKENKNEKF
                     LSQYPAVEGYPHFFVLDSDGKLLHSQNTGDLEKDKDYDKEKFTLFLEKWKGSKG"
     gene            complement(1461..2075)
                     /locus_tag="VF870_04955"
     CDS             complement(1461..2075)
                     /locus_tag="VF870_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX9251566.1"
                     /translation="MKILRNLYDWVLHWANTKYGAAALFLLAFAESSFFPIPPDALLI
                     ALVLGAQTKAFKFASICTVGSVLGALAGYAIGHFLWWTPSNEFTGIANFFFTNIPSFT
                     PAAFYKIQRMYEQYDFWIIFTAGFTPIPYKIITISSGAFNINIIMFIIASIISRGARF
                     FLVAFLIWKFGPQIKSFIDKYFNWLAMAFTVLLIGGFLVIKYFV"
     gene            2314..2898
                     /locus_tag="VF870_04960"
     CDS             2314..2898
                     /locus_tag="VF870_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9251567.1"
                     /translation="MKKFFSLLFLALFVGSISLSAQNFATKGTFEFGGSIGFSSTTDV
                     NNGESSDNSLSNFSFEPYVGYFVINSLELGIIPAFSTSSYGDNSTTSFGVYFAPAWNF
                     DLKSNLFPFLEGRIGYNTFSYDDGNSETDDPSNSGLAWGLRGGVKVQVGNSALVNLAL
                     SYDQITLNPENWDGDRNGANVLGINAGLTIFLGK"
     gene            3000..>3347
                     /locus_tag="VF870_04965"
     CDS             3000..>3347
                     /locus_tag="VF870_04965"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX9251568.1"
                     /translation="MKFERSAGILLHPTSLPGKYGVGDLGYDAYKFIDFLEKAGQKLW
                     QVFPLGPTGYGDSPYQCFSAFAGNPNLVSPDKLLEDDLLRDHDLQNIPKHDPTSIDYG
                     EVIEYKKSLLKKAF"
BASE COUNT         1103 a          566 c          544 g         1134 t
ORIGIN      
        1 ttagatgact cctacataaa tgattttgaa aaagcagttg atggaatgac ggttgaaaaa
       61 gcaaatgaga tcattaaaaa atattttcct aaaaacaatc tacaatttgt gctgattgga
      121 aaagcggatg acattcgtga aatagcaaaa aagtatggaa aggttatcga gaaaaatatt
      181 actgatgatg ggttttagtt tattaatgct tttgaattaa gaattaaagc acgaaagaat
      241 ttttttcgtg cttttttatt taaagtaaaa agttatttca tcaacataaa ttttaatttt
      301 acttagtatc ggattttttg tggaaacggt tttatctccc actatctggt tccattcgtt
      361 ttcaaataaa tctttataat cataatagag ttgaattgaa taacaatctg attcaagatt
      421 tcctttttca aaaactgcat agccgccgta atcccaatca aacccggaaa aagtaaaatg
      481 tttaccgtta agtcttacta attctttcaa ttccgttccg attttaattc cgttatcaag
      541 ttcccaaagt gatttatcgc catgtatttc aataaaatcc ggatttttaa aatttacagt
      601 atccttccat tttataaaaa aagactgagg agtatcttta aaaaacactg accccacaac
      661 tcgttctgtt ccttcggcgt accatctttc aacacgctca acattttttt tgccgacaat
      721 tcttacaaga tcggcttctg atgttttaga atttaaaatg ctgttgccga tgcacggcgt
      781 taatataaaa tcgcttttct gctgcgagta tgctgattgt aaaactaaaa ctatcagcaa
      841 ggggattata tgcttcatta aattttccac aatataaatt gcaaaagttt acatctgata
      901 aattaaaaat ctttttttat tatccttttg atcctttcca cttctcaaga aacaaagtaa
      961 atttttcctt atcgtaatct ttatcttttt caagatcacc ggtgttttga gagtgcaata
     1021 gctttccatc gctatcaagc acaaagaaat gcggataacc ttccactgca ggatattgag
     1081 agagaaattt ttcatttttg ttttctttac tgtaattgat tttaacaaca ataaagtttt
     1141 cttcaagtaa tgatttaact tctgcagtat tatgcataaa ggcatctatt cgatgacacc
     1201 aaatgcacca ttcaccgccg acatcaagaa ttattctttt atttaatttg gttgccgaat
     1261 cgacagcaat cttaaggtca tcaaaaggat tgcgcgccgg gtcaaacttt aaagttgaat
     1321 cgcttacctg ggcagataaa gaaattgccg ctatcagaaa gaatgcaaat gccaaaaata
     1381 tttttttcat ataagttaaa tgtgttttgt ttaataaatt tttatttagc tgttgatgtt
     1441 taattaccat tttataaacg ttaaacaaaa tacttaatca ctaaaaaacc gccaatcagt
     1501 aaaactgtaa acgccattgc cagccagtta aaatatttat caataaaaga ttttatctgc
     1561 ggtccgaatt tccatatcaa aaaggcaaca agaaaaaacc gtgcaccgcg acttattata
     1621 gatgcaatta taaacatgat aatattaata ttaaatgcac ccgaagaaat tgttattatc
     1681 ttgtaaggaa ttggagtaaa tccagcagtg aatattatcc aaaaatcata ttgctcatac
     1741 attctctgga ttttataaaa tgcggcgggc gtaaaagagg gaatgtttgt aaagaaaaag
     1801 ttcgcaattc ctgtaaactc atttgaaggt gtccaccata aaaaatgacc aattgcatat
     1861 ccggctaaag ctccgagaac agatcccact gtacagattg atgcaaactt aaaggctttt
     1921 gtctgtgcac caagaaccag tgcaattaaa agtgcatcgg gtggaattgg gaaaaaagat
     1981 gattctgcaa aagccagaag aaaaagagca gctgccccat atttagtgtt agcccaatga
     2041 aggacccaat cataaagatt tcgaagaatt ttcaagaaaa acctaattta ttttatgtaa
     2101 acataaagaa atattgtttt taattgaaaa cttatcaacc tcaaacatat taccaaccaa
     2161 tttatttaca gcaaagcatt aagtaaccac cataactccc taaaaagaaa caaattagtt
     2221 ttgcaaaaat acttgacaaa ccgagatttt tacttaattt ttgtcagcca aaatattttg
     2281 gttcttaatt attaacatta taggaggtct gttatgaaaa agtttttttc tcttttgttc
     2341 ttggctttat tcgttggaag tatttctcta agtgcccaaa attttgctac caagggaacc
     2401 tttgagtttg gtggtagtat tggtttctcc agcacaactg atgtaaacaa tggtgaaagt
     2461 agcgacaatt cgctttcaaa tttttcattt gagccatatg ttggatattt tgttattaat
     2521 agccttgaac ttggaattat tcccgctttc tcaacttcta gctatggcga taactccacc
     2581 acatcttttg gtgtttattt tgctcccgct tggaattttg atcttaaaag taatctgttc
     2641 ccttttcttg agggaaggat tggttacaat accttttctt acgatgatgg taattccgaa
     2701 acagatgatc cttcaaacag tggtttggcc tggggtttaa gaggcggcgt aaaggttcaa
     2761 gttggtaata gcgccctcgt taatcttgca ttatcttatg atcaaataac tttgaatcct
     2821 gaaaactggg acggagatag aaacggggct aatgttttag gtataaatgc tggtttaact
     2881 atttttttag gaaaataata ttttagttta attgtttaat cctgctctgt aaaaggagca
     2941 ggattttttt ttgctcactc tctccttatt tttccccaac attttttaaa ggttatataa
     3001 tgaaattcga acgaagcgct ggcattttac ttcatcccac atcacttccg ggtaaatatg
     3061 gagttggtga tcttggttat gatgcctata agtttattga tttcttagaa aaagccggac
     3121 aaaaactctg gcaggttttt cctcttggtc caactggcta tggagattcg ccctatcaat
     3181 gtttttctgc ttttgcaggt aatccaaatc tcgttagtcc ggataaatta ctggaagatg
     3241 atttgcttcg tgatcatgat ttgcaaaaca ttccaaaaca cgatccaacc tcaatagatt
     3301 atggtgaagt aattgaatat aaaaaatcat tactaaaaaa agcgttt
//
LOCUS       DASWBY010000270         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_346735, whole genome shotgun sequence.
ACCESSION   DASWBY010000270 DASWBY010000000
VERSION     DASWBY010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346735"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..305
                     /locus_tag="VF870_04970"
     CDS             129..305
                     /locus_tag="VF870_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251569.1"
                     /translation="MKIDTSILRTIPNFGKMKGLSRQRIHILLKDKRFDTLEIDGVKF
                     IILNNKALKFKAQK"
     gene            complement(566..638)
                     /locus_tag="VF870_04975"
     tRNA            complement(566..638)
                     /locus_tag="VF870_04975"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(603..605),aa:Met,seq:cat)
     gene            complement(737..1006)
                     /locus_tag="VF870_04980"
     CDS             complement(737..1006)
                     /locus_tag="VF870_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251570.1"
                     /translation="MAELEIKCQHACKNTCATLNEALRRETAMVRFYESILDECNMPD
                     VSSYMAGLVEDKRKVILSLIQKLNEIHVRSQALDGIASSFNNIDG"
     gene            complement(1032..1595)
                     /locus_tag="VF870_04985"
     CDS             complement(1032..1595)
                     /locus_tag="VF870_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX9251571.1"
                     /translation="MTKEHEIIFAGFGGQGVLSMGRLIAYAGMIEGKEVSWMPSYGPE
                     MRGGTANCIAIVSDSRISSPIISKFDTAILLNQPSIDKFEKTVKPGGLLIYEQSTAVH
                     PPTRTDIEIVCIAGNEEADKLNAKQIANMFMVGAFLEKRPILKIETIVEALKKALPPR
                     RHNLIPLNEQALLRGKELVKLADQVSA"
     gene            complement(1628..>2112)
                     /locus_tag="VF870_04990"
     CDS             complement(1628..>2112)
                     /locus_tag="VF870_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9251572.1"
                     /translation="YVFSYQGDGDLAAIGTAETIHTVNRGENILMLFINNGIYGMTGG
                     QMAPTSLIGQVTSTSPYGREAAQVGNPIKIADMLCLLPGAYFVTRQAVYNPGGVRKLK
                     KALVKAFEYQKQKKGTCFIEVVSNCPSGWKMTPVETIKYLEEQMIPMYPLGDLKVPQN
                     "
BASE COUNT          608 a          405 c          366 g          733 t
ORIGIN      
        1 tttgctacta tgcttatcga tcttgtttgt attcatttgt ggccatcggc aaagcaaccg
       61 tagaaggcat tattttttac tgcattcgtt actattgaca ttggtcaatg aaattattaa
      121 gttgaattgt gaaaattgat acatcaatat taagaacaat tccgaatttt ggtaagatga
      181 aaggattatc tcgccaacgt attcatatcc tgctcaagga taaaagattt gatactctgg
      241 aaattgatgg tgtaaagttc ataattctaa ataacaaagc attaaagttc aaagcacaaa
      301 aataatttta tttcaaaact gagaactacc acaatgaaaa acattttgaa attagagaaa
      361 gctcgagcag tttaagggtg tggtatccct tcagttctgt ttgagctttt ttgtttttaa
      421 gttcattgaa ttgatgaaaa aagtaatgac tattttgatg actattatct gattttacac
      481 tcatcaaatg gtcagcaggt gagtctgatc acatccgttt taaaacgaaa aagcccgaat
      541 acttatcata ttcaggcttt cttagtagcg ggggcaggac ttgaacctac aaccttcggg
      601 ttatgagccc gacgagctac caattgctcc accccgcgat ataaaatcta atttttaagc
      661 gataaacgct gttttctttc caaaatttag tgcgcaaata tagtcaatca gctttgcgat
      721 tgcaaatcta ctctaactaa ccatctatgt tgttaaagct tgatgctata ccatctaatg
      781 cctgagatcg gacatgaatt tcattaagct tttggataag actaagaata acttttcttt
      841 tgtcctccac caatcctgcc atataagaac ttacatcagg catgttgcat tcatccagaa
      901 tgctttcgta aaatcttacc attgctgttt ctctgcgcaa tgcttcattt aaagttgcac
      961 aggtgttttt gcaggcgtgc tgacatttta tttctaattc agccatggct tcctcaggtt
     1021 ataattatta attatgctga tacttggtca gctagtttta ctaattcttt tccgcgaagt
     1081 aatgcctgtt cgttcaaagg tattagatta tgtctgcgcg gaggcaaagc ttttttaagt
     1141 gcttcaacta ttgtttcaat tttcaatatc ggacgttttt ctaagaacgc gccaaccata
     1201 aacatatttg caatttgttt tgcgttaagt ttatctgctt cttcattacc ggcaatacaa
     1261 acaatttcta tatcggttct ggtcggcgga tgcacagcgg ttgattgttc ataaattaat
     1321 agtccgcccg gtttaaccgt tttttcaaat ttatctattg aaggttgatt aagaagtatt
     1381 gcagtatcaa attttgatat gataggtgag cttattcttg aatcagaaac tattgcaata
     1441 caatttgcag ttcctcctct catttcgggt ccgtatgaag gcatccagct tacttcttta
     1501 ccttcaatca ttccggcata agctattaat cttcccatag aaagtacacc ctgcccgccg
     1561 aatcctgcaa aaattatttc atgttctttt gtcattttaa aattccttta tcttataata
     1621 aacagattta gttttgagga acctttaaat caccaagtgg atacattggt atcatttgtt
     1681 cctcaagata ttttattgtt tctaccggtg tcattttcca gcctgatgga cagttggaaa
     1741 caacttcaat aaaacatgtt ccttttttct gtttctgata ttcgaatgct tttactaatg
     1801 ctttctttag ttttcgaact cctccgggat tataaacagc ctgccttgtt acaaaatatg
     1861 caccgggcaa taagcaaagc atatcagcaa tcttaatagg gttgccaacc tgtgcagctt
     1921 ctcttccgta aggagaagta gaggttacct gtccaattaa actcgtggga gccatctgtc
     1981 cgccggtcat tccgtaaatt ccgttgttga taaagagcat taaaatgttt tcaccgcggt
     2041 ttacagtatg gattgtttcg gctgttccaa ttgcggcaag atcgccatca ccctgatatg
     2101 agaaaacata tt
//
LOCUS       DASWBY010000271         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_84437, whole genome shotgun sequence.
ACCESSION   DASWBY010000271 DASWBY010000000
VERSION     DASWBY010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84437"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /gene="tilS"
                     /locus_tag="VF870_04995"
     CDS             complement(<1..1102)
                     /gene="tilS"
                     /locus_tag="VF870_04995"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX9251573.1"
                     /translation="MNAIEQKIIRFIKENKLLSDGEKILVALSGGPDSVFLLHILNKY
                     KKKFKIELGAVHINHLLRGKDSGRDELFCKTICEELSIPFYCLRKNVKDYSKKNKISL
                     EVAGRKIRYDFFEKISKANKYDKIATAHNVDDNAETVLLNLIKGTGIKGIAGIPVRRN
                     NIVRLILSLTKKEILNYLNENKFEYRVDESNLSNDFERNFLRNEVIPLIQNNVNPSFA
                     NTALITSLNLQRLNLVLADFVSGLKLLVRVKRNKSISIPIEFIKKNNNFILSYTIKEI
                     IDENFSVKLESIDLKKILLLAQKQSGKSEELSEKLIALKERDEILIKQKIALKNAVKK
                     IVSVGSELKLDHKILSITEVEKKEIKIYKSKTI"
     gene            complement(1102..1722)
                     /locus_tag="VF870_05000"
     CDS             complement(1102..1722)
                     /locus_tag="VF870_05000"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9251574.1"
                     /translation="MFTGLVEEKGILKEKIPTGDGFQFVIEAKIVMKDLQIGSSVAVN
                     GCCLTVVKIDGNTFAVDTIEETLNKTNLGVLKQGMKVNLERPLAADARLGGHFVLGHI
                     DTTGKVEDVKELSNSHWLTISFPEKFKQYLIYVGSVAIDGVSMTVAELKDKSFSVGII
                     PHTWKETIFADKKVGDTVNLEFDVLGKYVERIMESKSQDESSIFLN"
     gene            complement(1797..2885)
                     /gene="ribD"
                     /locus_tag="VF870_05005"
     CDS             complement(1797..2885)
                     /gene="ribD"
                     /locus_tag="VF870_05005"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX9251575.1"
                     /translation="MADESYIQLTIEIAKKGEGSVSPNPLVGCVILKDNRIIGAGYHQ
                     KFGEDHAEINAINSSVESLEGSTLYVNLEPCSHQGKTPPCVDRIIKEKIKRVVIGTLD
                     INPLVSGNGVKALKKAGIDVKVGVLEKECIELNKFFFKFITKKLPYITLKAAQTLDGM
                     IADKYNSSEWISSRESRKYVHWLRAKYDAVLIGSETAGIDNPKLTVRMTEGRNPYRVV
                     LDSSLKLKSELNLFKNNSDNKTIVITSDKSKSKLKKINQLEKLGVKVIFAKSDIQGRI
                     QLKSVLKELAKLQLISVLVEGGSKVYSSFIKKNLYDDIYLFVSPKILGGGLNTFSEMN
                     INKLGDAAKLNINHIQKIGDDLLIELVK"
BASE COUNT          907 a          542 c          382 g         1115 t
ORIGIN      
        1 caatggtttt tgatttatag attttaattt cttttttttc aacttcggta attgataaaa
       61 ttttatgatc taactttaat tcacttccaa cacttactat ttttttaacg gcatttttta
      121 atgcgatttt ctgttttatt aatatttcgt cacgttcttt taatgcaatt aatttttcgg
      181 ataactcttc agattttcca gactgttttt gtgcaagcaa taatattttt ttcaaatcga
      241 ttgattcaag ttttacagag aaattttcat caatgatttc tttaattgta tatgagagaa
      301 taaaattatt attctttttt ataaactcaa ttgggattga tatactttta ttacgcttga
      361 ctcttacaag taattttaat ccgctgacaa aatccgccaa taccaaattt aacctttgca
      421 agttaagcga ggtaataagt gcagtatttg cgaaagatgg atttacatta ttttgtatta
      481 aaggaattac ttcatttctt aaaaagtttc tttcaaaatc attcgaaaga ttcgattcat
      541 caactctgta ttcaaattta ttttcattga gataattcag gatctctttt ttagtcagag
      601 ataagattaa tctaacaata ttgtttcgtc ttaccggaat tcctgcaata ccttttatac
      661 cggttccttt gatgagattc aataaaactg tttctgcatt gtcatcaaca ttgtgtgctg
      721 ttgcaatttt atcatattta tttgctttag aaatcttttc aaaaaaatcg tagcgaattt
      781 ttcttccggc aacttccagg gaaattttat ttttctttga gtaatctttt acattcttgc
      841 gaagacaata aaacggtatt gataactctt cacaaattgt cttacaaaaa agttcatctc
      901 ttccggaatc tttaccacga agcagatgat ttatatggac agcacctagt tcaatcttaa
      961 acttcttttt gtacttattt aaaatgtgca gcaaaaaaac agagtccggt ccaccgctta
     1021 acgcaacaag aatcttttct ccgtcagaaa gaagcttatt ctctttgata aatcttataa
     1081 ttttttgttc gattgcattc atcaatttaa aaagattgag gactcgtcct gacttttact
     1141 ttccataatt cgttctacat atttacccaa aacatcaaac tcaagattta ctgtatcgcc
     1201 aactttttta tctgcaaaaa ttgtttcttt ccaggtatgc ggaattatcc cgacagaaaa
     1261 ggatttatct ttcaactcag caaccgtcat acttacacca tcaattgcaa cagaaccaac
     1321 ataaattaag tactgtttaa atttttcagg aaaagatatt gtgagccaat ggctgttgga
     1381 tagttcttta acatcttcca cctttccggt ggtatcaata tgcccaagaa caaaatgtcc
     1441 gcctaatctt gcatcagcag ccaaaggtct ttcaagattt actttcatac cctgtttaag
     1501 cacaccaaga tttgtcttat taagtgtttc ttcaattgta tcaacagcaa aagtatttcc
     1561 atcaatctta acaacggtta aacagcatcc gttaacggca acactgctgc caatctgcaa
     1621 atctttcatt acaattttcg cttcaataac gaattgaaat ccatcgccag ttgggatttt
     1681 ttctttaagt attccttttt cttcaactaa accggtaaac attttatgcc cttaattttt
     1741 tagaatggta gtacaaagtt atgaatagaa attctaagat ttaatggtag aaaagattat
     1801 ttaacaagtt cgataagtaa atcatcacca attttttgta tatgattaat gtttaatttt
     1861 gcagcatcac caagtttatt aatattcatc tcagaaaatg tatttaagcc gcccccgagg
     1921 attttaggac taacaaataa ataaatatca tcatataaat ttttctttat aaatgatgaa
     1981 tataccttgc tgccaccctc aaccaatact gaaatcaatt gtagtttagc taattctttt
     2041 aatacacttt ttaattgtat tcttccctga atatccgatt ttgcaaatat tactttaaca
     2101 cctagttttt ctaactgatt gatctttttc aatttagatt tacttttatc tgatgttatc
     2161 acaatagttt tattatcaga attatttttg aataaattta attcactctt caacttcagg
     2221 cttgaatcca gcacaactct atatggattt cttccttcgg tcattcttac tgttaattta
     2281 ggattatcaa tccctgctgt ttcagaacca ataagcacgg catcatactt tgctcttaac
     2341 caatgcacat attttcttga ttctcttgaa gatatccact cagaagaatt atacttatcc
     2401 gcgatcattc catccagcgt ttgagcggct tttaaagtaa tgtatggaag tttttttgtt
     2461 ataaatttga agaagaattt atttaattcg atgcactctt tttcaagcac accaacttta
     2521 acatcaattc ccgctttctt aagagccttt acaccgttac cactaacaag cggattgata
     2581 tccagagtac cgattacaac tcgttttatt ttttctttga taattctatc aacacaagga
     2641 ggagtttttc cttgatgact gcagggttca agatttacat ataatgttga gccttcaagc
     2701 gattctacgg aagaattaat tgcgtttatt tcggcatggt cttccccaaa cttctgatga
     2761 tatccagccc cgattattct attgtccttt aaaattacgc accctacaag cggatttgga
     2821 ctaacgctgc cttcgccttt ttttgctatc tcaatcgtaa gttgtatgta tgattcgtca
     2881 gccaaaattg taacaattaa ttataaaata attatttgat ctccataatt tcaaattcca
     2941 ataaac
//
LOCUS       DASWBY010000272         4147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_688896, whole genome shotgun sequence.
ACCESSION   DASWBY010000272 DASWBY010000000
VERSION     DASWBY010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688896"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VF870_05010"
     CDS             <1..231
                     /locus_tag="VF870_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9251576.1"
                     /translation="ISVSKYVETIKGKVGIKIFQVFKGLMKQKYWGNHFWARGYFVST
                     VGIDEEMIRRYVKYQEKEEKKEEVQNDKFILF"
     gene            691..1269
                     /locus_tag="VF870_05015"
     CDS             691..1269
                     /locus_tag="VF870_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9251577.1"
                     /translation="MDTELTDIELIQRIINRDSKALESLYDRYSPVLYALVNRIVSDK
                     EKSEDILADIFVIIWQKSSMFDINTGNLYSWLINLTRNKALDFVRREKSLITDEYNDE
                     YENAVILPNISPLIPSNDLGKIFDSREKIFSAFHNLTEAQQYVLSLAYYDGLSESEIA
                     VKLNIPLLTVKSKVRVALNSIKEFLEKEVTND"
     gene            1262..2275
                     /locus_tag="VF870_05020"
     CDS             1262..2275
                     /locus_tag="VF870_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251578.1"
                     /translation="MIDENKVIGMLQAKALGCLDAEEDKELQEFIDAGHVFPWDEFGI
                     NQNLASLLPLALPLELPDAELKDRVALKLIKLSEQLRINKILEEDKFEVEEEFEENVN
                     EFTNIPEAFIDPPIEIETEETVVEDNLAETLNSDESNFNLDEIVLPEFEGGEVANENP
                     EESVNIESPFETLVDEAQIDYPKVEEAPIDELLTEPEIQPIKKTAQEDISIKHIISEA
                     NSVKDSFKSLKTDKSEEIEISNDQPDLTKRSVAEKMFKAIEQDFDSLKYHYEESERKT
                     TRGLLFAYVVIGLLIALLIFSFFKFSSDINNLENEVKELKSKLSSSLYQEQKTNSDHS
                     FFS"
     gene            complement(2247..2774)
                     /locus_tag="VF870_05025"
     CDS             complement(2247..2774)
                     /locus_tag="VF870_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251579.1"
                     /translation="MKFFKSVNYYLLMLFIVVTLLSLLRVIPLSALEIAAYTCLFWGI
                     SFFYSSYLKQYKTGIVIGSISFLTGSILFVFTRFEILSFGTVFIPSALIIIGLSLLIS
                     NLLTETNSTSVIFSILSMFAGIWLTISRGTATVELYLTAVYDLLRSYWIIVLILAAII
                     FLTGRNFTKRKNDQN"
     gene            complement(2771..3694)
                     /gene="rocF"
                     /locus_tag="VF870_05030"
     CDS             complement(2771..3694)
                     /gene="rocF"
                     /locus_tag="VF870_05030"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEX9251580.1"
                     /translation="MIKEKQQTVTIIGFPIDLGSGRRGVDMGPSALRIAGLEGKLEQL
                     GYNVEDTGDINIQIMERQKISNPKLKYLDEILKTSKMLAARVEKVLEQNKFPLCIGGD
                     HSMALGTIAGISSYCKRNKLTLGVIWIDAHTDMNTDETTPSGNIHGMPLAASMGLGYP
                     ELVNLYGFAPKLQPENCAVIAARSIDPQERLNIKKLGVNVYTMSDVDKLGVHRIISRV
                     LKQFKEKVDHIHVSFDVDSVDPNFAPGVGTPVPGGLNYREAHSLMEAIAECGCMSSLE
                     IAEVNPILDVRNSSAVFAADLIASSMGQRIL"
     gene            3827..>4147
                     /locus_tag="VF870_05035"
     CDS             3827..>4147
                     /locus_tag="VF870_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9251581.1"
                     /translation="MLGYDSEPYISVGILTDDKIKFELYGEYSSYGFKHFFSGRYEAE
                     LVNDRIICRGDKDKIEITDEIIFEPQEPDNESFLIKDVVIGVKFHWERKEKQRFTHSL
                     KLIKD"
BASE COUNT         1490 a          628 c          728 g         1301 t
ORIGIN      
        1 atatcagtga gcaagtatgt agaaacaata aagggaaaag ttggaataaa gatattccaa
       61 gtatttaagg gtttaatgaa acagaaatat tggggcaatc atttttgggc gaggggatat
      121 tttgtcagta ctgtaggaat agatgaagaa atgataagaa gatatgtgaa ataccaagag
      181 aaagaggaaa agaaagaaga agtacagaat gataaattta tcctctttta agaggtaaga
      241 aaaaaagaaa gccaccacct atggcggtgg tagtttactt aaaaactagc aataataaaa
      301 aagatagaat tatttgtgcg taatttaaaa ttataaataa ataattttaa attatttatt
      361 ggtagtctca tataatacta ttttaaccta accattctgg ttcgctttta ataaagcctg
      421 ctgtaatctt gagcttactt caacttgtga atatgtaaga gaagcagtaa tgagtagaac
      481 gaaaaatagt ataagttttt tcaatatatc actcctttat gttttacgtt caaatttaac
      541 atgtaattat taatgcttca actttttaaa aaatttaagt tgataatttg taattgtgtt
      601 attatcaaat gagctaatga ttgctttttt gaatgcagac atatactttt agcatggtaa
      661 aatgatttag aactaaatat tattaaaata gtggatacag agttaacaga tattgaatta
      721 atccagagaa ttattaatcg tgattctaaa gcgctggaaa gtttatacga tagatacagt
      781 cctgtgttgt acgctttggt taatcgcata gtctctgata aagaaaaatc agaggatata
      841 cttgcagata tttttgtaat aatctggcaa aagagctcaa tgtttgatat taataccggt
      901 aatctttatt cctggttaat aaatcttacc agaaataaag cccttgattt tgtcagacgt
      961 gaaaaatcat tgataactga tgagtacaat gatgaatatg agaacgcagt tatactccca
     1021 aatatttctc cgcttatccc atccaatgat ttaggtaaaa tttttgacag cagggaaaaa
     1081 atattttcgg catttcataa tcttaccgaa gcacagcaat atgttttatc cttggcttat
     1141 tacgatggat taagtgaatc agaaatcgct gttaaattga acattccatt attaactgtt
     1201 aaatctaaag tacgggttgc actaaacagc attaaagaat ttcttgaaaa agaggtaacc
     1261 aatgattgat gaaaataaag ttattggaat gcttcaggcg aaagctcttg gttgtcttga
     1321 tgctgaggaa gataaagagc tgcaggaatt tattgatgca gggcatgttt ttccgtggga
     1381 tgaattcggg attaatcaaa acttagcttc tcttttacct cttgctttac cattagaact
     1441 acctgatgct gaattaaaag atcgtgttgc attaaaactt ataaaactaa gcgaacaatt
     1501 aagaatcaat aaaatccttg aagaagataa gtttgaagtt gaagaagaat ttgaagagaa
     1561 cgttaatgaa tttacaaaca ttcctgaagc ttttattgat ccgcctatag aaattgaaac
     1621 agaagaaaca gtagttgagg ataatttagc tgaaacatta aattccgatg aaagtaattt
     1681 taatttagat gaaattgttt tacctgaatt tgaaggtggt gaagtagcta atgaaaaccc
     1741 ggaagaatca gtaaatattg aatcaccatt tgaaactctg gttgatgagg ctcaaattga
     1801 ttatccgaaa gtggaagaag caccaataga tgaactatta acggaacctg aaattcagcc
     1861 aataaaaaaa acagcccagg aagatatttc aattaaacat atcatttcag aggcaaatag
     1921 cgttaaggat tcattcaaga gtcttaaaac tgataaatct gaagaaattg aaatatcaaa
     1981 tgatcagcct gatttaacta agagaagtgt tgctgaaaaa atgtttaagg ctattgagca
     2041 ggattttgat tcgttaaaat atcattacga agaaagtgaa agaaagacga cacgcggact
     2101 tttgtttgca tatgttgtaa tcgggctgct aattgcactg cttatttttt cattctttaa
     2161 gttcagctct gatataaaca atcttgaaaa tgaagtgaaa gaattaaaga gtaaactctc
     2221 atcctcttta tatcaggagc agaaaactaa ttctgatcat tctttctttt cgtaaaattt
     2281 cttcctgtta aaaaaattat tgctgctaga attaaaacta taatccagta acttcttaat
     2341 aaatcataaa ctgcagttaa atataattcc acagttgcag tgccgcgtga aatcgttaac
     2401 caaattcctg caaacatgct taatattgaa aagattacag aagtgctgtt tgtttctgtt
     2461 aacaggtttg aaattaataa acttaatcct ataataatta gtgcggaggg aataaacaca
     2521 gttccgaaac tcaatatttc aaaacgtgtg aacacaaata atattgatcc agtaagaaaa
     2581 gaaatagaac caattacaat tcctgtttta tattgtttaa ggtaagatga atagaaaaaa
     2641 ctgattcccc agaataaaca ggtgtaagca gcaatttcca atgcactgag aggaatgact
     2701 cttaacagac ttagaagtgt taccacaata aaaagcatca acaaataata attaacagat
     2761 ttaaaaaatt tcacaggatt ctttgcccca tacttgatgc aattagatca gcagcaaaaa
     2821 cagcgctgct gttacgcaca tccagaattg gatttacttc agcaatctcc agtgaagaca
     2881 tgcatccgca ttcagcaatt gcttccataa gtgagtgtgc ttctctgtaa tttaatccac
     2941 cgggtacagg cgtgccaaca ccgggtgcaa agttcggatc aacgctgtca acatcaaaac
     3001 taacgtgaat gtggtctact ttttctttga attgttttaa cactcggctt ataattctat
     3061 gcactccgag tttatccaca tcgctcattg tataaacatt tacaccaagt tttttaatat
     3121 ttagtctttc ctggggatca atacttcttg ccgctataac agcacaattt tcaggttgta
     3181 atttgggcgc aaatccataa agattaacaa gctcaggata gcctaatccc atagaggcag
     3241 caagcggcat accgtgtata ttaccggaag gggttgtttc atcagtgttc atatcagtat
     3301 gcgcgtcaat ccatataaca cccaacgtta gtttgttcct tttacagtaa gaagatatac
     3361 ctgcaattgt gccgagtgcc attgaatgat caccgccgat acagagtgga aatttatttt
     3421 gctctaaaac tttttcaact cgtgcagcaa gcattttaga tgtcttaaga atttcatcta
     3481 agtatttaag ttttggatta gagattttct gccgttccat aatctgaata ttaatatcac
     3541 ccgtatcctc aacgttgtaa ccaagctgct caagtttacc ttctaatccg gcaatgcgca
     3601 atgcagaagg tcccatatca acgcctcgcc ttcccgaacc cagatcaata ggaaaaccaa
     3661 ttatcgtaac tgtttgctgt ttttctttta tcatagaaat tttttaaata atattaaatc
     3721 attttaataa agttaaggag attgggggaa atattcatcg ttacaattct tatattttag
     3781 caacaaaaat tattctttat ttaaagacaa taaaacgggc aaaaatatgt taggatatga
     3841 cagtgaacct tatataagtg ttggaatctt aacagatgac aaaataaagt ttgaactata
     3901 tggcgagtac agttcttatg gattcaaaca tttttttagc ggcagatatg aagctgaact
     3961 tgttaacgat cggattatct gcaggggtga taaagataaa attgaaataa ctgatgaaat
     4021 tatttttgag ccgcaggaac ctgataatga atcttttctt ataaaagatg ttgtgattgg
     4081 tgtaaaattt cattgggaaa ggaaagaaaa acagcgcttt acacattcac ttaaacttat
     4141 taaagac
//
LOCUS       DASWBY010000273         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_346755, whole genome shotgun sequence.
ACCESSION   DASWBY010000273 DASWBY010000000
VERSION     DASWBY010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346755"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VF870_05040"
     CDS             complement(<1..330)
                     /locus_tag="VF870_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251582.1"
                     /translation="MKNLICALAFLLFTTSSFSQWQYRHPYIKQIDINQIKSSLSNFG
                     TLGLEYLETTFWNQLQYRDYLTGRGLNNTEIVYDQGLWMLGKINGEKHASVTEWNTHF
                     SPGPIING"
     gene            complement(401..1087)
                     /locus_tag="VF870_05045"
     CDS             complement(401..1087)
                     /locus_tag="VF870_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251583.1"
                     /translation="MSNEILKKLNYTGKFLIIVILFAIAVPFTNSNAQGIGELAPPKP
                     AEVFPPNAWGMDIMFGDAGFGLGTFYRREITTKWTAFADLSFSETKDDREFEYIDYFG
                     NVITIGKKNRVFQMPLNFGVQLRLFENSIADNLRPFVCAAAGPTLLITTPYQEEFFNS
                     FAYAQTEYAVGGYVGLGANFGLDKSSLVGITFKYYYSKILSGGVESLYGREKTEIQGF
                     FITLNLGMMY"
     gene            1194..1703
                     /locus_tag="VF870_05050"
     CDS             1194..1703
                     /locus_tag="VF870_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEX9251584.1"
                     /translation="MPKNLEIKVKLSSHKEVQEILKKNKIPFEELLLQKDIYYKVNKG
                     LLKLRIENDKQTLIFYDRNEKSKKRWSDYHLLEIDKADANKYLKRFLEVLTIVNKKRE
                     LYLYKNTRIHLDYVKGLGSFLELETRVVNGLKDAEKRFAYLMDLLKLRDKKEIRASYK
                     DLLLLKDKK"
     gene            1700..>2827
                     /locus_tag="VF870_05055"
     CDS             1700..>2827
                     /locus_tag="VF870_05055"
                     /inference="COORDINATES: protein motif:HMM:NF045064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9251585.1"
                     /translation="MILSKNKIERIDPDNLINSFISQAKLDEILIIVPTNRKIRYLKR
                     ELVSQSPGKSVSKINLHTFETFTNQIFQLDDFHSFKTLSEASSAVLLSKSFKKTELKY
                     FSNYKNEIPRGTLDRIKNVITQYKLNGISPVQINSEASNLGGSEKLKAIDIANVYKNY
                     LAYCKELDVYETGDVYSEVLLLDVKNFTERFNKSFSGIKTILINGFDEFTQLEIDIIN
                     RAAEIEGIELYVMFDYYRYNPALFSHLDSCYEKFKARGFSEVEDTSPIQFNDYQKRIR
                     EKLFLLNKNDTPKPSEIEAVKIVAQSPEEEIRLIAKEIKSLITKKNVDVDSITVAFNL
                     ISDHSSIIRDVFNEYGIPFNLTDRYALNESQPVIALINFLEI"
BASE COUNT         1053 a          435 c          440 g          899 t
ORIGIN      
        1 accattaata attggaccag gagaaaaatg tgtattccat tcagtaacac tagcatgctt
       61 ttctccattt atttttccaa gcatccataa cccttgatca tatacaatct ctgtattatt
      121 taaccctctt ccagtgagat aatctctgta ttgtaattga ttccaaaagg tagtttctaa
      181 atattctaat ccaagtgttc cgaaattact tagtgaagat tttatttggt taatatcgat
      241 ttgttttatg tatgggtgac gatattgcca ttggctgaat gatgatgttg tgaataaaag
      301 aaaggcaagc gcacatatta gatttttcat tatttccctt aaaaacctac ctgatatatt
      361 gtagaggact tacatatacc aaagctaaat tgaacaagaa ctaatacatc attcccaaat
      421 taagcgtaat aaaaaatcct tgtatttcag ttttttctct tccgtaaaga ctttctactc
      481 caccagaaag aatcttagaa tagtaatatt taaaggtaat ccccactaag cttgatttat
      541 ccaagccaaa atttgcacct agtccaacat aacctccaac cgcatattcg gtttgtgcat
      601 aagcaaagga gttaaaaaat tcttcctgat atggcgtagt aataagcagt gttggacctg
      661 cagcggcaca aacaaaaggt cttaaattat cggcaataga attttcaaaa agtctaagtt
      721 gcacaccaaa atttagaggc atctgaaaaa ctctgttctt ttttccaatt gtaattacat
      781 taccaaaata atctatatac tcaaactcac ggtcatcttt agtctcagaa aatgaaagat
      841 cagcaaaggc agtccattta gtggttattt ctcttctata aaaagtacca agtccaaaac
      901 cggcatcacc gaacattata tccattcccc aggcattagg cggaaaaact tctgctggtt
      961 ttggcggcgc aagttcgccg ataccctgag cattcgaatt cgtaaaaggt actgcaattg
     1021 caaataaaat tacgatgatt aaaaattttc ctgtataatt cagttttttc aatatttcgt
     1081 tagacatttt aatattctta tttttctatt ggtaaaataa ttattgaaat gacaatttca
     1141 atgtattatt taatcattat gtaaaaaaca ctctaaaagc agaaaacaat caaatgccta
     1201 aaaaccttga aataaaagta aaattatctt cacacaaaga agtacaagaa atacttaaaa
     1261 agaataaaat tccttttgag gaattattat tgcaaaagga tatttattat aaggtaaata
     1321 aaggattgtt aaagctgagg atcgagaatg ataaacagac tttgatattt tatgaccgaa
     1381 atgaaaaatc taaaaaacgc tggtctgatt atcatctttt agagatagac aaagctgatg
     1441 cgaataaata tctaaaaaga tttttggaag ttcttacgat cgtaaacaaa aaaagagaat
     1501 tatatcttta caagaacaca agaatccatc tggattatgt aaaagggtta ggcagttttt
     1561 tagaattgga aactcgagtt gttaatggtt taaaggatgc tgaaaaaaga tttgcctatt
     1621 tgatggattt actaaagctt agggacaaga aagaaattag agcttcttac aaagatttac
     1681 ttttactaaa agataaaaaa tgattctttc taaaaataaa attgaacgga tagatccgga
     1741 taatcttata aatagtttta taagtcaggc aaaacttgat gagattttaa taatcgtacc
     1801 gacaaacaga aagataaggt acttaaagag ggaattggtt tctcaatcgc ccggcaaatc
     1861 agtttcaaaa ataaatctcc acacttttga aacttttaca aatcagatat ttcagctgga
     1921 tgattttcat tcatttaaaa ctcttagtga agcatcttcc gcagtgctgt taagcaaatc
     1981 atttaagaaa acagaattaa aatatttttc taattataaa aatgaaattc cacgcggcac
     2041 tttagacaga attaagaatg taattactca atacaaatta aacggtattt cacctgttca
     2101 aattaattca gaagcaagca atcttggcgg atctgaaaaa ttaaaagcaa ttgatatcgc
     2161 aaatgtttat aaaaattatt tagcgtactg caaagaatta gatgtttatg aaacaggtga
     2221 tgtttattca gaagttcttt tgcttgatgt aaaaaatttt actgaaagat ttaataagtc
     2281 attcagcgga ataaaaacaa tcttaattaa tgggttcgat gaatttaccc agctggaaat
     2341 tgatataatt aatcgagctg cagaaattga aggcatcgaa ctttatgtaa tgtttgatta
     2401 ttaccgatac aatcctgcat tgttttcaca ccttgatagc tgttacgaaa aatttaaagc
     2461 caggggtttt agtgaagttg aagacacatc cccaattcag tttaatgatt atcagaaaag
     2521 aatacgtgag aaattatttt tattgaataa aaatgatacg ccaaaaccga gtgaaattga
     2581 agctgtgaaa atcgttgcac agtctcccga agaagaaata agacttattg ccaaagaaat
     2641 aaaatcactt atcacaaaaa agaatgttga tgttgattca ataaccgtag cttttaattt
     2701 aatatcagat cattcgtcaa taattcgcga tgtttttaat gaatatggaa ttccatttaa
     2761 tcttacagac agatatgcgc ttaatgaatc acagccggta attgctttga taaactttct
     2821 ggaaatt
//
LOCUS       DASWBY010000274         6506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_12220, whole genome shotgun sequence.
ACCESSION   DASWBY010000274 DASWBY010000000
VERSION     DASWBY010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12220"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VF870_05060"
     CDS             complement(<1..1341)
                     /locus_tag="VF870_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9251586.1"
                     /translation="MSEMENYFSQFRKNIVGVDTTFSTPFGEQKLIYADWIASGRLYK
                     PIEEKLCEKFGPFVGNTHSEASVTGTTMTLAYHEAHKIIKDHVNACKDDVIITTGSGM
                     TTVVCKFQRLLGLKIPEQLIDYVKLPAELKPVVFITHMEHHSNQTTWLETIADVVVIN
                     PNEEGLIDINDFKAKLEKYKNRKLKIGSFTAASNVTGIEPDYYELSKMIHQYGGYSFI
                     DFACAAPYVKIDMHPADPEAKLDAIFFSPHKFLGGPGTPGVLVFDSKLYHNRVPDLPG
                     GGTVDWTNPWGQHKFVSNIEAREDGGTPAFLQTIKAALCIKLKEEMGVDNIRARENEL
                     VRIAFAGLKKIPTLHILAGNIEHRLGAISFYIEDIHYNLIVKILNDRFGVQVRGGCSC
                     AGTYGHYLLHVDPNRSKIITDKIDQGDLSEKPGWVRMSIHPTMTDEELNIALDGI"
     gene            1460..2605
                     /locus_tag="VF870_05065"
     CDS             1460..2605
                     /locus_tag="VF870_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9251587.1"
                     /translation="MKIRIKIFAARILILALRVGAQSNAYIFNDVDEVINKAIEDKSF
                     PGAVVLVWKDGKTVYEKAFGNFTYDRSSPKVKTTTIYDLASVTKVVATTTAAMICYDR
                     NLFSLDYKVVKYIPEFGVNGKENITIKNLLIHNSGLPAWKKFYERDLNYDDVINEIYS
                     SELEYKTGEKTVYSDLGIITVGKIIEKVTGRSLDKFCEDEIFIPLKMNSTFYNPNDSV
                     KQFCAPTEIDNYWRMKTLQGEVHDETSAMLNGVAGHAGLFSTASDISKLMAMLMNKGE
                     LDQKQFIKQSTIELFTKKVSTNSTRAIGWDTKSDSGSSSGTYFSKDSFGHTGYTGTSI
                     WADPVRNLFVVFLTNRLYPTRENIKIQKVRPQLHDAVIKCLERINAK"
     gene            2595..4400
                     /locus_tag="VF870_05070"
     CDS             2595..4400
                     /locus_tag="VF870_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9251588.1"
                     /translation="MRNKLLLKNNLGFLSLVTLQFFFLFLPIVKSEVSSYPEEKTNTN
                     TEDVFNLSKEDELWVENTLESMTIYDKCAQLFMPAVFGKSLDQSSYEFKSALELVKLL
                     GIGGIVISTGDVDKTASMINELQRNSKIPLLVGADFENGIGMRMNASNTFPHSMAVGS
                     TNNINFAYQTGKTTAIEALMLGVNFNFAPVADINNNPENPVINLRSYSEDKDIVTDFC
                     KAYVEGSIEGGVIASAKHFPGHGNTKVDSHNDLPVINGDQKYLYENELKPFIELIKNG
                     VQAIMIGHLNVPAFEPDKDLPSSLSYNIITKLLKEKLEFKGLIITDALDMKAVTKYYS
                     TSEICLKAFKAGNDILLMPSNIRDGITAIYDAVNKGEITEYRLNESVRKILSAKRWLK
                     LDQAKYRENKNLPKRIRMEEHYQLSKTIAEKSVTVVKMNNELIPFDSSKYKKTFIVNI
                     TNRKSISDPHFIEAYKENFSLYSSTTLNNSSKNRDYKAAMNVAKDCELIIVASYFSIK
                     NDDNGNALSETQLKFFDDLLALKKRVIIISFENPYILSSFPQAENYICTFSNSKSSQR
                     AVVNFLKGSIEPTGRLPVSIPNTGYQIGFRWEPNS"
     gene            4423..5526
                     /locus_tag="VF870_05075"
     CDS             4423..5526
                     /locus_tag="VF870_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX9251589.1"
                     /translation="MKNIFIVLLIITFIISCNSETESQSSSIKLPPGFKIEIFASNVP
                     NARSMVLSKSGILFVGTRSAGNVYALVDNDKDFKVDEVITIASGLNMPNGVDLKDGDL
                     YVAEVSRIIKFENIENTFRNNSDYIVVRDDFPTDKSHGWKFIKFGPDGKLYVPVGAPF
                     NICLKENEDARYASITRMNADGSDFEIYARGIRNTVGFDWHPNTNELWFTDNGRDMLG
                     DNIPPDELNLAPLLGLHFGYPFLHGKNILDPEFGSQGDTSLYTKPVQELGPHVAALGM
                     EFYTGHMFPAEYQNQIFIAEHGSWNRSEKIGYRISLVKLDGNNSVSYETFADGWLNGD
                     TVSGRPVDIELMPDGSMLVSDDYANAIYRITYK"
     gene            5627..6154
                     /locus_tag="VF870_05080"
     CDS             5627..6154
                     /locus_tag="VF870_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9251590.1"
                     /translation="MIILFNKPFNVLCQFSDKEGRQTLSDFLQIKKVYAAGRLDYDSE
                     GLVVLTDDGKLQNKISDPKHKLVKTYWVQVEGIPGEESLKELRTGIMLKDGLTKPAKA
                     KLIEEPKIWERIPPIRERKNIPTSWIELIISEGKNRQIRRMTAAVGYPTLRLVRLSIG
                     DWTIENLNPGNHIII"
     gene            complement(6161..>6506)
                     /locus_tag="VF870_05085"
     CDS             complement(6161..>6506)
                     /locus_tag="VF870_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX9251591.1"
                     /translation="LDAINHPLAIFAGIILSGAVVTDFPPLIKWALALIAGGGIAGTI
                     HAATGLIRLKSSALTGGAGNPVVSTAEAGSSVALSVIAIFIPAVAVLIVGIMIVYFSF
                     MIKKKFFSPPTE"
BASE COUNT         2247 a         1040 c         1155 g         2064 t
ORIGIN      
        1 aattccgtcc agtgcaatat ttaactcttc atctgtcatg gttggatgaa ttgacattct
       61 tacccaacct ggtttttcag atagatcacc ttggtcaatt ttatctgtaa taattttaga
      121 gcgatttgga tcaacgtgca ataagtaatg tccgtaagtt cccgcacagg aacaaccgcc
      181 tcttacctgc acaccaaaac gatcatttaa tatttttaca attaaattgt agtgaatgtc
      241 ttcaatataa aatgaaattg ctccaagcct atgttcgata ttacccgcca gaatatgcag
      301 agttggaatc ttttttaatc cagcaaaagc aattcttaca agttcgtttt ctcttgctct
      361 gatattatca acacccattt cttcttttaa ttttatacat aatgctgctt taattgtttg
      421 aagaaacgca ggtgtgccgc catcttcacg ggcctcaata ttggaaacaa atttatgctg
      481 tccccatgga ttagtccaat ccacagttcc gccgccgggt aaatccggaa ctctgttgtg
      541 ataaagtttt gaatcgaaga ccaaaactcc aggtgtcccc ggtccgccta agaatttgtg
      601 tggggaaaaa aatattgcat ccagctttgc ttctggatca gcaggatgca tatctatttt
      661 aacataaggt gcggcgcagg caaaatctat aaatgaatac ccaccatatt gatgaatcat
      721 tttggataat tcatagtaat ccggctcaat gccagttacg tttgaagcgg cggtaaatga
      781 accaatcttt aattttctgt ttttgtactt ttctaatttt gctttaaaat catttatatc
      841 aattaatcct tcttcgtttg gattaattac aacaacatct gcaattgttt ctaaccatgt
      901 agtttgattt gaatggtgct ccatatgagt tataaaaact accggtttta attctgcggg
      961 aagttttaca taatctatta gttgttcggg aatttttaat ccaaggagcc tttggaactt
     1021 acaaacgacg gtcgtcattc ctgatcccgt agtaataatc acgtcatctt tgcaagcatt
     1081 aacgtgatct ttaataattt tatgagcctc atgataagct aatgtcatag tggtgcctgt
     1141 aacactagcc tcagagtgag tgttgcctac aaacggtcca aatttttcgc aaagcttttc
     1201 ttcaattggt ttatatagtc ttccgcttgc aatccagtct gcataaatta atttttgttc
     1261 accaaacggg gttgaaaaag ttgtatcaac tccaactatg tttttgcgga attgagaaaa
     1321 ataattttcc atttcactca tacataaacc tattcatata taactgaaat ataataattt
     1381 aagacgagtg aatcattgaa ttttttcgtt tattcatttt cattaaatta tagtcaaaat
     1441 tatctaaata ataaaaaaaa tgaagattag aattaaaata tttgcagcac gaattttaat
     1501 attagcatta agggtgggtg cccaaagtaa tgcttatata tttaacgatg ttgatgaagt
     1561 catcaataaa gcgatagaag acaaatcttt tcccggcgca gtggttcttg tctggaaaga
     1621 tggaaaaact gtttatgaga aagcatttgg aaattttaca tatgatagat cttctccaaa
     1681 agtaaaaaca actacaattt atgatcttgc gtcggtaaca aaagtagttg ctaccacaac
     1741 cgcagcaatg atttgctatg atagaaattt attctcgcta gattataaag ttgtaaaata
     1801 tatcccggaa ttcggtgtta atggaaagga aaacataaca attaaaaact tactcattca
     1861 caattcggga ttgcctgcct ggaaaaagtt ttatgagagg gatttaaatt atgatgatgt
     1921 aattaatgag atttattctt cggaacttga atataaaacc ggagagaaaa ctgtttactc
     1981 agatttagga atcattactg taggaaaaat tattgaaaaa gtaaccggaa gaagtttgga
     2041 taaattttgc gaagacgaaa tttttattcc cttaaaaatg aattcgacat tttacaatcc
     2101 aaacgattca gttaaacaat tctgcgctcc aacggagata gataattatt ggagaatgaa
     2161 aactctgcaa ggtgaggttc acgatgaaac ttctgctatg ctaaatggag ttgcagggca
     2221 cgccggttta ttttctactg caagtgatat ttcaaaatta atggcgatgt tgatgaacaa
     2281 aggtgaactc gatcaaaaac aatttataaa acaaagtacg attgaactat ttactaaaaa
     2341 agtttcaact aacagcacaa gagcaattgg ctgggatact aaatcagatt ctggttcttc
     2401 gtccggaacc tatttctcta aagattcttt tggacacact ggttatacag gaacttcaat
     2461 ctgggctgat cctgtaagaa atctgtttgt cgtttttctt acaaatcgtt tatatccaac
     2521 aagagaaaac ataaagattc aaaaagtaag acctcaatta cacgatgcag taataaaatg
     2581 tttagaaagg attaatgcga aataaattat tactaaaaaa taatttggga tttttgagtt
     2641 tagtaacgtt gcaattcttc tttttgtttt taccaattgt taaaagtgaa gtttcatcat
     2701 atcctgagga aaaaacaaat acaaataccg aagacgtctt taatctttcc aaagaagatg
     2761 agctatgggt tgaaaatact ttagaatcaa tgaccatata tgataagtgt gcacagttat
     2821 ttatgcctgc agtttttgga aaaagtcttg atcaatcatc ctatgaattt aaaagtgctt
     2881 tagaattagt taagcttctc ggtatcggtg gaatcgtaat ttcaactggt gatgtggata
     2941 aaaccgcatc gatgattaac gaacttcaga ggaattctaa aattcctttg ctggtaggcg
     3001 ccgattttga gaatggaatt ggaatgcgaa tgaatgcttc taatactttt ccgcatagca
     3061 tggcagttgg ttccacaaac aatattaatt ttgcttatca aacaggtaaa acaactgcca
     3121 ttgaagcctt aatgcttggt gtaaatttta attttgcacc tgttgcagat attaataata
     3181 acccggaaaa tcctgtaatt aatcttcgct cttattctga ggataaagat atagtaacag
     3241 atttttgtaa agcatatgtt gaaggatcaa ttgagggtgg agtaattgct tcagcaaaac
     3301 attttccagg tcatggcaac acaaaagttg attctcataa cgatctacct gtgataaacg
     3361 gcgatcaaaa atatttatat gaaaatgaat tgaaaccttt tatagaattg attaaaaatg
     3421 gtgttcaagc aattatgata ggccatctta atgtacctgc ttttgagccg gataaagatc
     3481 tgccatcatc gttatcatat aacatcataa caaagctctt aaaagaaaaa ttagaattta
     3541 aaggtttgat aattaccgat gcgcttgata tgaaagctgt aacaaaatac tactcaacca
     3601 gtgaaatatg tttaaaagca tttaaagcgg gtaatgatat tttgctgatg ccatcaaaca
     3661 tacgtgatgg aattacagca atttatgatg cagttaataa aggggagatt acagagtaca
     3721 gattaaacga aagtgtaaga aaaattttat cagctaaaag atggcttaaa cttgatcagg
     3781 ctaaatatag agaaaataaa aatcttccta aacgaattcg gatggaagaa cattatcagc
     3841 tatctaaaac catcgcagaa aaatctgtta ctgttgtaaa gatgaataat gaattaatcc
     3901 cttttgattc atcaaagtat aaaaagactt ttattgtaaa tattacaaac agaaagagta
     3961 ttagtgatcc tcattttatt gaagcttata aagaaaattt ttctttatac agcagcacaa
     4021 ctcttaacaa tagctcaaag aatagagatt acaaagcagc tatgaatgtt gcaaaagatt
     4081 gtgagctgat aatagtagcc tcttattttt ctattaaaaa tgatgataat ggaaacgctc
     4141 tttctgaaac acagcttaaa ttttttgatg atttattagc acttaaaaag agagtaataa
     4201 ttatcagttt tgaaaatcca tatattttat cttcgttccc tcaggcagaa aattatattt
     4261 gtacattcag taattcaaag tcatcacaaa gagctgttgt taattttttg aagggctcaa
     4321 tagaaccaac aggcagatta cctgtttcta tcccaaacac aggatatcaa ataggtttta
     4381 ggtgggaacc aaattcctga ttataaatta aatggcagct ttatgaaaaa tatttttatt
     4441 gtcttattaa taattacttt tataatttcc tgtaattcag aaacagaatc tcaatcatct
     4501 tcaattaagc ttccacccgg atttaaaatt gaaatatttg cctcaaatgt tccaaacgct
     4561 cgttcaatgg tattaagcaa aagtggaatt ctttttgtcg gaacaagaag tgctggaaat
     4621 gtatatgctt tagttgataa tgataaagat tttaaagtcg atgaagtaat cactattgca
     4681 tccggtctta acatgccaaa tggagtcgat cttaaagacg gcgatttata tgtagcagaa
     4741 gtaagccgca ttattaagtt tgagaacata gaaaatactt ttcgaaataa ttcagattat
     4801 attgttgtcc gtgacgattt tccaactgat aaaagtcatg ggtggaagtt tattaaattt
     4861 ggcccggatg gaaaacttta tgttccggtc ggagcacctt tcaatatttg tttaaaagaa
     4921 aatgaggatg cccggtatgc ttctatcaca agaatgaatg ctgatggaag tgattttgaa
     4981 atttatgcaa gaggaattag aaacactgtt ggatttgatt ggcatccaaa tacaaatgag
     5041 ctttggttta cagataacgg aagagatatg cttggagata acattccacc ggatgaatta
     5101 aaccttgccc ctttactggg attacatttc ggttatccat ttcttcatgg aaaaaatatt
     5161 cttgaccctg aatttggatc tcagggagat acttcactat atacaaaacc tgttcaggaa
     5221 cttggacctc acgttgcggc tttgggaatg gaattttata ccggacatat gttccctgct
     5281 gaatatcaaa accaaatatt tattgcagag catggaagct ggaaccgaag tgaaaagatt
     5341 ggttatagaa tttcacttgt taaattagat ggaaataatt ctgttagtta cgaaactttt
     5401 gcagatggct ggttaaatgg tgatactgtt tccggtcgtc cagtagatat tgaattaatg
     5461 ccggatggtt ctatgctggt ttctgatgat tatgccaatg ctatttacag gataacttac
     5521 aagtaaaacg agaccatttt gaatacagaa tatcgaatga tgaattttga gtaatgtagt
     5581 taactttaat tcgatgttct ttattcaaaa tttaaagtaa aaaataatga taatcctttt
     5641 taacaaacca tttaatgttc tatgtcagtt ttctgataag gaaggaagac aaactctttc
     5701 agatttttta caaattaaaa aagtttatgc tgcaggaaga ttagattacg atagcgaagg
     5761 attggttgta ttaactgatg atggtaaact tcaaaataaa atatcagatc caaaacataa
     5821 actggtaaaa acatattggg ttcaggttga agggattccg ggtgaagaat cattgaagga
     5881 acttagaaca ggaattatgc ttaaagatgg attaacaaaa ccagcaaaag ctaaattaat
     5941 tgaagaacca aaaatctggg aaagaatccc gccgataaga gaacgcaaaa atattccaac
     6001 aagctggata gagttaataa tatctgaagg caaaaacaga caaattagaa gaatgacagc
     6061 agcagttgga tatccgacat taagattagt acgtctctca atcggcgatt ggacgataga
     6121 gaatctaaat cctggtaatc atattattat ttaaagatta ctattcagtt ggcggtgaaa
     6181 aaaacttttt cttaatcata aaggaaaagt atacaatcat aatcccaaca attaaaaccg
     6241 caactgccgg aataaatata gcaattacag atagcgccac tgaactgcct gcttctgcag
     6301 ttgaaactac gggatttcct gccccgccag tgagcgcaga tgattttaac ctaatcaatc
     6361 ctgtagctgc atgaattgtt cctgctatgc ctcctcctgc aattaatgcc agtgcccatt
     6421 taattaacgg aggaaaatcg gttactacag caccacttaa aattatcccc gcaaatattg
     6481 caagcggatg attgattgca tctaga
//
LOCUS       DASWBY010000275         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_46637, whole genome shotgun sequence.
ACCESSION   DASWBY010000275 DASWBY010000000
VERSION     DASWBY010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46637"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..978
                     /locus_tag="VF870_05090"
     CDS             139..978
                     /locus_tag="VF870_05090"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX9251592.1"
                     /translation="MDLYNKTSFAISKVVTKSYSTSFSLGIAAFSPQYRDAIYGIYGF
                     VRIADEIVDTFNEFDQKQLMENFRKETEEALKTGISTNPILQAFIQTVKEYKIDYHYI
                     DAFLKSMEMDLTNSYYEPQQYNEYIYGSAEVVGLMCLQIFAGEDTKLFNDLVAPARSL
                     GSAFQKVNFLRDIKSDMEERERIYLPGVNHAIKIDDMHKQDLEKEIELEFKNALEGIK
                     KLPVGVKLGVYSAYLYYKGLFKKIKKLKVKELMNQRVRVSNPTKLALLIRGLLEIKVF
                     KLT"
     gene            1033..1704
                     /locus_tag="VF870_05095"
     CDS             1033..1704
                     /locus_tag="VF870_05095"
                     /inference="COORDINATES: protein motif:HMM:NF039186.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEX9251593.1"
                     /translation="MSTYLIINILIIAVPLILSFDKNLKFYKKVPYVLQSIAFISTAY
                     IVWDVIATKRGDWAFNPEHLVGFYILGLPIEEILFFITVPYSCIFIYETASFYVKEKK
                     FNFNYKLFLIPAISVMILGIIFYDQNYTFTVSIFTGAFFLGVVLFNNSLPNSRNFWIT
                     MLISFLPFLIVNYFLTSIPVVTYNDAAFSGKRFITIPYEDFLYSFSMISLWILFYDLA
                     KEKKK"
     gene            1701..>2079
                     /locus_tag="VF870_05100"
     CDS             1701..>2079
                     /locus_tag="VF870_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9251594.1"
                     /translation="MKKKICVIGAGLGGLSAAIRLANKGFEVDLYEQNNIPGGKAGEI
                     NENGYRFDTGPSLLTMPQVIKDLFSECDENIEDYFKINRLELICKYFYLDKSIINAYS
                     DAEKFGKEIEEKTSDNTDSLNKYL"
BASE COUNT          705 a          318 c          358 g          698 t
ORIGIN      
        1 ttggtgcatt cagacccaaa aacaaagacg aagattttcc aaatctttat tatgtaggtg
       61 cttcaacaac accaggcaca ggtttaccaa tggttatcat cagctcaaaa ttagtaacag
      121 atagaatcag gagtgattat ggatctatat aacaagactt cgttcgctat tagtaaggta
      181 gtaaccaaaa gttacagtac ttctttcagt ctgggcatcg ctgctttctc tcctcaatac
      241 cgtgacgcta tttacggcat ttacggtttt gttagaatag cagatgaaat tgtcgacacc
      301 tttaacgagt ttgatcaaaa acagttgatg gaaaactttc gaaaagaaac agaagaagct
      361 ttaaaaacag ggatatcaac aaacccaatt ttgcaagctt tcattcaaac ggttaaagaa
      421 tacaaaattg attatcacta catcgatgct ttcttaaaaa gtatggagat ggatttaact
      481 aattcctact acgagcctca gcagtacaac gaatacattt atgggtccgc agaagtcgtt
      541 ggattgatgt gtttgcaaat ttttgccgga gaggatacta agttattcaa tgatttggtt
      601 gcacccgcaa gaagtctagg ttcagctttt cagaaggtaa acttcttaag agatattaaa
      661 agtgatatgg aagaaagaga gagaatttat cttcccggtg ttaaccatgc aattaaaata
      721 gatgacatgc acaaacaaga tttagaaaaa gaaattgagc tggaatttaa aaatgcttta
      781 gaaggtatta agaaattgcc tgttggcgta aagctcggtg tttactcagc atacctttat
      841 tataaaggat tattcaaaaa aataaaaaaa ttaaaagtaa aagagctgat gaatcagcgt
      901 gtacgagtat ccaacccaac taagttggct ttgttaataa gaggtttgct tgaaataaaa
      961 gtttttaagc taacctgaaa caaaattaaa atttaattaa gttgccggta gcaataccgg
     1021 cttttctttt atatgagcac atatttaatt ataaatattt taataatagc agttcctctg
     1081 attttatcat tcgataaaaa tctgaaattc tataaaaaag taccttatgt tttgcagtca
     1141 attgctttca taagcactgc ttacatcgtc tgggatgtga ttgcaacaaa acgcggcgat
     1201 tgggccttta atccagaaca tcttgtcggc ttctatattt taggcttacc gattgaagaa
     1261 atattatttt ttataactgt tccctactcc tgcattttta tttatgaaac agcttccttc
     1321 tatgtcaaag agaagaagtt taattttaat tataaattgt ttttaatccc tgcaatatca
     1381 gttatgattt taggaataat tttttatgat caaaattata cttttacggt ttctattttt
     1441 acaggagcat tttttcttgg tgttgtttta tttaacaatt ctttgcctaa ttccagaaac
     1501 ttctggataa ctatgcttat tagttttctt ccttttctga ttgtcaatta tttccttact
     1561 tcaattcccg ttgttactta taacgatgct gccttcagtg gtaaaagatt tattactatt
     1621 ccttatgaag attttcttta ctcattttca atgatttctt tgtggatatt attttatgat
     1681 ttagcaaagg aaaagaaaaa gtgaaaaaga aaatctgtgt tattggtgct ggacttggcg
     1741 gattatcggc agccattaga cttgcaaaca aagggtttga agttgatttg tatgaacaaa
     1801 ataatatacc aggaggcaaa gccggtgaaa taaacgaaaa tggttatcga tttgacaccg
     1861 gcccatcttt gttaacaatg ccccaggtta ttaaagattt gttttcggaa tgcgatgaaa
     1921 atattgaaga ctatttcaaa ataaacaggc tagaattaat ttgtaaatat ttttatctag
     1981 ataaatcaat cataaatgct tattctgatg ctgaaaagtt tgggaaagaa attgaagaaa
     2041 aaacttctga taacaccgat tcattaaata aatacctgc
//
LOCUS       DASWBY010000276         6455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_84486, whole genome shotgun sequence.
ACCESSION   DASWBY010000276 DASWBY010000000
VERSION     DASWBY010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84486"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VF870_05105"
     CDS             <1..463
                     /locus_tag="VF870_05105"
                     /inference="COORDINATES: protein motif:HMM:NF028498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HEX9251595.1"
                     /translation="DKNLYFTEQWTGVNGVFADELKWNIRELTVGASRNWCKTMLEWN
                     LAADQNQEPHTPGGCTECLGAITIQGDQVTRNVAYYITAHASKFVRPGSKRIESNYFS
                     GLPNVAFLTPSGNVVVIVLNNSASFKTFNIKLGNNKFKTYLNAGAVGTYIW"
     gene            complement(579..1655)
                     /locus_tag="VF870_05110"
     CDS             complement(579..1655)
                     /locus_tag="VF870_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX9251596.1"
                     /translation="MKKQLIIRNLLSFVLFMLSGFLFISNAADKEKTFNVSKGDMLNV
                     SASNGNVNISIWSKDQAYIKAFNIDEDDLNDLKIEQTGNKVVVDFKGQDSDDFYLEIS
                     IPTYMHLDISSGAGNISVSGNAAGKVEIATGGGNINLNDVGDKLSVSTGGGNVSVGNI
                     NGESEISTGGGNISIGDAKNKTEVSTGGGNISIGNIGGSGEVSTAGGNISVGKVSGNA
                     ELSTAGGNINLDGASGKVETNTAGGNINLKNISGSIEANTAGGNIYAELMPTANSNSE
                     FNTASGDINLKIPSDAKVSIEATVYVAKNLSEADADKLIKSDFEPTNVDFHKNEFIKK
                     FVLNGGGSMIELNTASGKIKISKK"
     gene            complement(1657..2208)
                     /locus_tag="VF870_05115"
     CDS             complement(1657..2208)
                     /locus_tag="VF870_05115"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9251597.1"
                     /translation="METDANNSEKLRIQNINFIDSDPSDGQVEFTFDAVKSGRIKGNV
                     NDAEVQKILTYAVLNEQNPGTRLNSINVINANQIQKPDDEIKSTLIAVAKFDNNPGVR
                     IEALKSLNKLPADEEIKNTLIYVLLNDTSSGIRIEAINSLVGASKSGVSLNQKDLMLL
                     RDKIQTENNNYVKFQAKNIIKEY"
     gene            complement(2212..2589)
                     /locus_tag="VF870_05120"
     CDS             complement(2212..2589)
                     /locus_tag="VF870_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9251598.1"
                     /translation="MNHNECKELIPFYLYEELDPEKKKLFDDHLKSCKDCTNELESYK
                     NLFADISEDSKYPIDPKLLLEARLELRGAFRAQQTKTSISTSITNYFTSFIYKPVGLA
                     ISGLSVMLTGLVIGYLIFNSPVI"
     gene            complement(2613..3176)
                     /locus_tag="VF870_05125"
     CDS             complement(2613..3176)
                     /locus_tag="VF870_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9251599.1"
                     /translation="MALSDNELIINANKGDVLALEELIYRYDKMVLRLSMKYTGDSDD
                     AKDIYQEVFIRVYKSLSSFQFKSEFSTWLFRITSNVCISFKRKYKKQNHLSLNDEENS
                     GYISTITEDEVLSPDNVFVNSEHGEKINNALNTLSPNQKMSFLLKHYEGYKIREIAEM
                     MNCKEGTIKKYLFDAVRKLKTQLEKSY"
     gene            complement(3615..>6455)
                     /locus_tag="VF870_05130"
     CDS             complement(3615..>6455)
                     /locus_tag="VF870_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX9251600.1"
                     /translation="QIMDKAEKLINKDNSILSKVYVSKGSLYLLSGMYDEAEKFYKKG
                     FSSAKLTGDKREEIKALINLAILDDQSGNVEQAKQKLESALQASRSIKNQELEATALS
                     ELAVSYTYSGNVVEAKNYYEESFKMFKILNHKERLSNLCANIASLYSQTANYSSAIEY
                     YNNGLDYSGENVVSSILNLRGLGDVYSNLSNYSKSLNYYDEAKKLSQQIKDVALETSV
                     DVSFGTLYYNINKPVKALQLFIEASKKLDLDNDPYSAEDIFFKIGLAYSKIDSLSKSN
                     EALLNALSLAESLSDVYYKTLISTELGHNYALQKDFSKAESEIKKASVLSKSNGFIQI
                     LGLQNLYLGSIAVARGNLVNAIKYFETASSLATKEMDFNNILEAEYLLGKTYKTQNNI
                     IKAEEHYLNAVNLADRISESLINNAEIQISHFSGLNDCYNELAELYLEQNRNEDAFCV
                     IEKSRSRNTFQNLADLKINTFFSNKEILNHFYDLKWMVNSGLFSGNKLEEINKEYAIL
                     KEEISKANPELNSTLEKNKLFSIKEIQKSLNNKENLITLFFGKTNLFAINITRDKSTV
                     IKVDVSKDETIKFLRNIAPIYSPDSNSADLYLNQDLFSFNTKEANLFYKKIIEPVIDG
                     IPNGETLIFSMPSELAFVPIEFLVTEFNEDDSPFYYENKKFLIDNYPISYAPSASIYV
                     IQKQIIQQNENKILLVGDPQVSNKDFALSYRGSLLEADNFNARNIVLFPLKYSREEIQ
                     SLGSLLSNGLVFLSDNATERNFKENASNCRIIHLSTHSFLLKNQPLIIFSQNGDTKED
                     GFLETGEILKLKLKSDLVVLSSCRSGLGSVDNAEGVLGMQKSFFEAGAKSVVVSLWDV
                     NDKYTSLFMQSFYNFLSEGFDKSESLQKAKLFFKKNYSSNPYYWSAFVLSGDISNIQN
                     IKSVSNNYILYTLVGVIISILLIYFYLKKSRI"
BASE COUNT         2080 a         1082 c          994 g         2299 t
ORIGIN      
        1 ggataaaaat ctttacttta ccgaacaatg gacgggtgta aatggagttt ttgcagatga
       61 attaaaatgg aatattagag aattgacagt aggtgcatca agaaactggt gcaagacaat
      121 gctggaatgg aacttggccg cagaccaaaa tcaggaacct catacacccg gaggatgtac
      181 agagtgcctt ggtgccatta cgatacaggg cgatcaggtt acacgcaatg ttgcatatta
      241 tataacagca cacgcatcaa agtttgtaag accaggctcg aagagaattg aatcaaatta
      301 tttttctgga ctaccaaatg tagctttctt aacaccttcc ggtaatgttg tggttattgt
      361 attgaataac agtgcttcat tcaaaacttt taatataaag ctggggaata ataaatttaa
      421 aacttatctg aacgcaggag ctgttggaac atacatatgg taacgcagaa aatgagacga
      481 ggataaatta tgcttcgcag atttttcgag caaaagtgct atatttatag aaaccgcctg
      541 atgattattt ttatctcacc aggcggaaat ttagaaattt atttcttact tatttttatt
      601 ttaccggagg cagtgtttag ttcgatcata gagccgccgc cattaagaac aaacttttta
      661 ataaattcat tcttatgaaa atcaacatta gttggttcaa aatcagattt aattaatttg
      721 tcagcgtcag cctcactcag atttttagca acatagacag tggcttcaat gcttacttta
      781 gcatcggagg gtattttgag gtttatatcc ccgcttgcgg tattaaattc actattacta
      841 tttgcagtcg gcatcagttc agcataaata ttacctccgg ctgtattagc ttcaattgaa
      901 ccggaaatat tttttagatt tatatttcca ccggctgtat tagtttctac ttttccggaa
      961 gcaccatcta aattaatatt tccaccggca gttgataatt ctgcattgcc ggaaactttc
     1021 ccgaccgaaa tatttccacc cgccgtggat acttctccgc taccgccgat gtttcctata
     1081 gatatatttc cgcctccagt agatacttcg gttttatttt tagcatcacc aatactaatg
     1141 tttccgccgc ccgttgagat ttctgattca ccattaatat ttcctacaga aacattcccg
     1201 ccgcctgtag aaacagaaag tttatctcca acatcattta aatttatgtt accgccgcca
     1261 gtagcaattt caactttgcc ggcagcattt ccactaaccg aaatattgcc agcaccgctt
     1321 gaaatatcca aatgcatata ggttgggatt gatatctcga gataaaaatc atccgagtct
     1381 tgtcctttaa aatctacaac aactttattg ccggtctgtt cgattttaag atcatttaga
     1441 tcatcttcat caatgttgaa ggctttaata tatgcctgat ctttggacca aattgatatg
     1501 ttaacatttc catttgaagc agatacatta agcatgtcac ctttagaaac attaaatgtt
     1561 ttttctttat cagccgcatt tgagataaat aaaaatcccg ataacataaa tagaacgaac
     1621 gaaagcaaat ttctgataat taattgcttt ttcattttag tactccttta taatattctt
     1681 tgcttgaaat tttacgtaat tattgttctc tgtttgaatt ttatcgcgaa gcagcattaa
     1741 atctttttga tttaagctaa caccggattt agaggcaccg accaaactat tgattgcttc
     1801 aattctaatt cctgaactcg tatcgtttaa cagcacataa atcaaagtgt ttttaatctc
     1861 ttcatcagca ggcagtttat ttaatgactt tagagcttct attcttacac ccggattatt
     1921 atcaaatttt gcaacagcaa ttaaagtgct tttaatctcg tcatccggtt tttgaatttg
     1981 atttgcatta atgacattta tagaatttaa tctggtacca ggattttgtt catttaaaac
     2041 cgcataagtt aatattttct gtacttcagc atcattaaca tttcctttaa ttcggcctga
     2101 tttaacggca tcaaatgtaa actcaacctg tccgtcagaa ggatcggaat caataaaatt
     2161 tatattctga attcttaatt tttcagaatt attagcatct gtttctatct tttaaattac
     2221 aggcgaattg aaaatcaaat aaccgattac aagtcctgtt aacattaccg ataaaccact
     2281 tattgctaat cccacgggtt tataaataaa tgaagtaaaa taatttgtaa tgcttgttga
     2341 gatagaagtt tttgtttgtt gtgctctaaa tgcacctcgc aattctaatc gtgcttctaa
     2401 taataatttg ggatcaatag gatattttga atcttctgaa atatcagcaa aaagattttt
     2461 ataggattct aattcatttg tgcaatcctt gcaggatttt aaatgatcat caaataattt
     2521 tttcttttca ggatccaatt cttcatatag atagaagggg atcaattctt tacattcgtt
     2581 gtgattcata acttattcca ttagacaaaa tattaataac ttttttctaa ttgtgttttt
     2641 agttttctaa cagcatcaaa aagatatttt tttattgtac cctctttgca attcatcatt
     2701 tcagcaatct ctctgatttt atatccttca taatgtttta atagaaaact cattttctga
     2761 ttcggtgata aagtatttaa agcattattt atcttttcac catgttcaga attaacaaat
     2821 acattgtcag gagataaaac ttcatcttca gtaattgttg agatatatcc tgagttttct
     2881 tcatcgttta aagaaagatg attttgtttt ttgtattttc ttttaaaact tatacaaaca
     2941 ttcgaagtaa ttctgaacaa ccaggttgaa aactcactct taaactgaaa acttgataaa
     3001 cttttataca cccgaataaa aacttcctga taaatatctt tggcgtcatc cgagtcgccg
     3061 gtatacttca tagaaagtcg caggaccatt ttgtcatagc gatatatcaa ctcctcaaga
     3121 gctagcacat cacctttgtt tgcgtttatt attagttcgt tatcgctcaa tgccattaaa
     3181 atattctgtt aacaatttat tagaccaaag ttacatagaa atggttggtg ggtaaaagcg
     3241 attatttaat tgaaaaaatg aattaaggtt ttataaataa aaatttgatg cagatgacga
     3301 aacggattgg tgttatattt attaatagat agcggaatag atcttgtttt taaaagctta
     3361 aaagcctcaa gaacgcatat tttgaactta attgaggctt aattttaagt agcggagagt
     3421 cagggaattt ttgtgaggct aaaaaaagtg tctttattga ttaaaaacgc atcttttcta
     3481 aaatgtcttc taaatgtctt ttaccttaaa tatcgagttt atggtgttga aaaatatttt
     3541 gttcaggtct aaaagattag ggaagtaata tttaatttta tgcataatca aatttcattg
     3601 attaaaacaa aaagttaaat acgggatttt tttaagtaga aataaatcaa taaaatagag
     3661 attattactc caacaagggt ataaagaatg taattgttag aaacagattt aatattttgg
     3721 atattagaaa tatcaccgga caaaacgaac gcagaccagt aatatggatt tgaagaatag
     3781 ttcttcttaa agaataattt agctttttgt aatgattctg atttgtcaaa accttcacta
     3841 aggaaattgt agaagctttg catgaacaaa gatgtatatt tgtcgttcac atcccacaat
     3901 gaaaccacta cgctttttgc accagcttca aagaaagatt tctgcatacc gagcactcct
     3961 tcagcgttat caacactgcc caatccagag cggcaggagc ttagaacaac taaatctgat
     4021 tttagtttta atttaagtat ttcacctgtt tcgagaaaac catcttcttt tgtatcccca
     4081 ttctgtgaaa aaataataag cggttgattt tttaatagaa atgagtgtgt tgataaatgt
     4141 ataattcggc aatttgaagc attttcttta aagtttcttt cagtagcgtt atctgataag
     4201 aacactagtc cattagaaag caaagagcca agactttgaa tttcctctct agaatatttc
     4261 agcgggaaaa gcacaatgtt tcttgcatta aaattatcag cttcaagcaa actgccccta
     4321 taactaagag caaaatcttt attgctaact tggggatcac caacgagcaa aattttattt
     4381 tcgttttgtt gaattatctg tttttgaata acataaatcg atgcagaggg ggcatatgaa
     4441 ataggataat tatcaataag gaattttttg ttttcatagt aaaacggact gtcatcttca
     4501 ttaaattcgg ttacaagaaa ttctatcgga acgaatgcta attctgaagg cattgaaaat
     4561 ataagcgttt cgccatttgg aatgccatca attactggtt caattatttt tttataaaac
     4621 aagttagctt ccttggtgtt aaaagaaaac aaatcctggt ttaagtataa atcggcactg
     4681 ttagaatcgg gagaatatat aggcgcaata tttcttaaga atttaatcgt ttcgtcttta
     4741 gacacatcaa cttttattac agtcgattta tctcttgtta tattaatcgc aaataaatta
     4801 gttttaccaa aaaaaagagt tatcagattt tctttattgt ttagactttt ttgaatttct
     4861 ttaattgaaa acagtttgtt cttttctaag gtggagttta attccggatt tgccttagaa
     4921 atttcttctt ttaatatagc atactcttta ttaatctctt ctaatttatt acccgagaac
     4981 aatcccgagt ttaccatcca tttaaggtca taaaaatgat ttagtatttc tttgttgcta
     5041 aaaaatgtat tgattttaag atcagcaaga ttttgaaaag tgtttcttga acgtgatttt
     5101 tcaattacgc aaaatgcatc ttcatttcta ttttgttcca gatacaattc ggctagctca
     5161 ttataacaat cattgagacc tgaaaaatgc gaaatttgga tctcagcatt attgattaat
     5221 gattcggata tcctatcagc aagattgacc gcattaagat aatgttcttc tgctttgata
     5281 atattatttt gagttttata agttttccca agcagatatt cagcttctaa aatattatta
     5341 aaatccattt cttttgtggc cagtgatgaa gccgtttcaa aatattttat tgcattaaca
     5401 agattgcctc tggcaacagc aattgaaccc agatataaat tttgtaggcc caatatctga
     5461 atgaatccat tacttttaga taatactgaa gcttttttta tttcgctttc cgctttggaa
     5521 aaatcttttt gcaatgcata attatgcccc aactcagttg agattaatgt tttataatat
     5581 acatcagata aagattcagc taagctgagt gcatttagta atgcctcatt gcttttactt
     5641 aaactatcaa ttttagaata agccaatcca attttgaaaa aaatatcttc tgcagaataa
     5701 ggatcattat ccaaatcaag ttttttactt gcttcaataa ataactgaag ggcttttacc
     5761 ggcttattaa tattataata aagcgttcca aagcttacat caaccgaagt ttctaatgca
     5821 acatctttta tttgctgaga aagctttttt gcttcatcat aataattaag tgatttggaa
     5881 tagttagaaa ggtttgagta gacatctcca agtccacgca gatttaatat gctggaaaca
     5941 acattttcgc ccgagtaatc taatccattg ttataatatt ctattgcgct agaataatta
     6001 gccgtctgtg agtataatga agcaatatta gcacaaagat ttgaaagcct ttctttgtga
     6061 ttcaggatct taaacatttt gaagctttct tcataataat tcttggcctc aacaacatta
     6121 cccgagtaag tataagatac tgccaattct gataaagcag ttgcttcaag ttcctgattt
     6181 ttaatgcttc gagatgcctg caatgcagat tccaattttt gttttgcctg ctcaacattc
     6241 ccactctgat catcaagtat tgcaagattt ataagagctt taatttcttc tcttttatcg
     6301 ccagttagct ttgcactact aaagcccttt ttataaaact tttcagcttc gtcatacatt
     6361 ccagaaagca aataaagtga gcctttagat acataaactt tactgagtat tgaattatct
     6421 ttatttataa gtttttctgc cttatccata atttg
//
LOCUS       DASWBY010000277         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_272148, whole genome shotgun sequence.
ACCESSION   DASWBY010000277 DASWBY010000000
VERSION     DASWBY010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272148"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VF870_05135"
     CDS             <1..507
                     /locus_tag="VF870_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9251601.1"
                     /translation="STTRAVEDIANNHNCISFRSAVGEANVVQKMKEMNAVIGGEGSG
                     GVIYPALHYGRDALVGIAFTLQYLADSGKKLSEIKDELPQYFIAKKKIELKDVSPDDV
                     INSLIKKYSKENINTEDGLRIDYEDHWVHFRKSNTEPIVRIITESKTQKYSEQLSQKY
                     YDEINELM"
     gene            complement(499..1407)
                     /locus_tag="VF870_05140"
     CDS             complement(499..1407)
                     /locus_tag="VF870_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9251602.1"
                     /translation="MSKSFSEYKSYLDPKIISRIGSLDLRARLVVEGFMVGLHKSPYH
                     GFSVEFSQHRPYMQGDNLRSVDWRVFGKTEKYFIKQYEEETNLRSYIILDSSKSMSYS
                     SDNSISKLDYASTLVAALSYLMIKQQDAVSLTIYSDKIHKHLPPKSSNSYFQQILKEL
                     SVLETSSNTNTADSLNQVAEKIKRRGLVIIISDFFDDIDKTLKAIKHFAYQKNEVIIF
                     QILDPMERSFGFGKDAIFKDLETNEELTTQPFQIQKAYQQAMNEFTNKIKRQCLNSNF
                     DYNLLDTSTPFDKALFSYIQKRSRLH"
     gene            complement(1404..>1738)
                     /locus_tag="VF870_05145"
     CDS             complement(1404..>1738)
                     /locus_tag="VF870_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9251603.1"
                     /translation="APKALIQLAQLYQNQLVEKIKPSESFDKAEKYFMQVYEKYPDSE
                     EAPKALFMSGFILANDLKKYDEATARYKLFLEKYPSHPLAVSAKEELDNMGLSPEEIL
                     KRTETVEK"
BASE COUNT          566 a          288 c          291 g          593 t
ORIGIN      
        1 tcaacaacaa gagcagtcga agatattgct aataatcata attgcatttc attccgctct
       61 gctgttggcg aagcaaacgt tgttcaaaaa atgaaagaaa tgaatgcagt aatcggaggt
      121 gaaggaagcg gcggagtaat atatccggca ttacattatg gaagagatgc attagttggt
      181 atagcattta ctttgcaata tttggctgac tcaggcaaga aactttctga aattaaagat
      241 gaactacctc aatattttat cgcaaagaaa aaaattgagt tgaaggatgt ttctcccgat
      301 gatgtgatta atagtctcat taaaaaatat tctaaagaaa atattaatac tgaagatgga
      361 ttgagaattg actacgaaga ccattgggtg catttcagaa aatctaacac tgaaccaata
      421 gttagaataa ttacagaatc aaaaacacaa aaatattctg agcagctttc tcaaaaatat
      481 tatgatgaga ttaatgaatt aatgtagtcg tgatcttttt tgtatgtaac taaagagtgc
      541 tttatcgaat ggagttgatg tatcaagaag attgtaatcg aaatttgaat tcaaacattg
      601 tcttttaatt ttgtttgtaa actcattcat agcctgctga tatgcttttt gaatctgaaa
      661 cggttgggtt gttaattctt cattagtttc caagtcctta aatatagcat ccttcccaaa
      721 accaaaactt ctttccatgg gatccagaat ctgaaaaatt attacttcat ttttttgata
      781 tgcaaaatgt ttaatggctt ttaaagtctt gtcaatatca tcaaaaaagt ctgaaataat
      841 aattactaat cccctcctct taatcttttc cgctacctga tttaaagaat ccgcagtgtt
      901 tgtatttgat gatgtttcta aaaccgacaa ctcttttaat atctgctgga aatatgaatt
      961 tgaagacttt ggaggcaagt gtttatgaat tttatcagag tagattgtaa gactaacagc
     1021 atcctgctgt ttaatcatta agtaagatag tgcagctacc aatgttgaag cataatcaag
     1081 tttggatatt gaattatctg aagagtaact cattgattta ctgctatcca gaatgatgta
     1141 gcttcttaaa ttcgtttcct cttcgtactg cttgataaag tatttttcag tcttaccaaa
     1201 tacccgccaa tccacgcttc ttagattgtc accctgcata taaggtcgat gctgcgaaaa
     1261 ttcaacactg aatccgtgat aaggactttt gtgtaatcca accataaatc cttcaacaac
     1321 caatctggca cgtaaatcca gactgccgat acgtgaaatt attttcggat cgagataact
     1381 tttatattct gaaaaagatt tactcacttt tctacggtct cagttcgttt aagaatttct
     1441 tccggggata atcccatatt atctaattct tcttttgcgg aaactgctaa tgggtgagag
     1501 ggatattttt ctaaaaataa tttatatctg gcagttgctt catcatattt ctttagatca
     1561 tttgctaaaa taaatccaga cataaataat gcttttgggg cctcttcgct atccggatac
     1621 ttttcataaa cctgcataaa atatttttct gctttatcaa aggattcaga tggcttgatc
     1681 ttttcaacca attgattttg atataattga gccaactgaa tcaatgcttt tggggcct
//
LOCUS       DASWBY010000278         4972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_385024, whole genome shotgun sequence.
ACCESSION   DASWBY010000278 DASWBY010000000
VERSION     DASWBY010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385024"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VF870_05150"
     CDS             complement(<1..785)
                     /locus_tag="VF870_05150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9251604.1"
                     /translation="MSKLITEKKFLIDVPEGKRKERIDTFLTHHIENTTRSKVQKAID
                     AGFVKVNGVVVKSNYNVKPFDKVEAVQLISPRPEDVEAEAIPLDIIYEDEYFIIVNKP
                     AGMVAHPAYANYTGTLVNALLHHTKSLSTVNEGGRPGIVHRIDKDTSGLLVVAKDDFT
                     HAKLAAQFSKHTIEREYHAICWGKFKEKEGEISTKITRSKSDRKKFTISDKEGKDATT
                     FYKVIEQYEFTSYIKLNLKTGRTHQIRVHLSGLHKPIFGDHTYG"
     gene            complement(748..1458)
                     /locus_tag="VF870_05155"
     CDS             complement(748..1458)
                     /locus_tag="VF870_05155"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX9251605.1"
                     /translation="MKALYLSFAVVVIDQVSKLMVKGFSIPFLNINYEGMYLGQMIPV
                     FGDFFRLTFVENPGMAFGYDPGSNFKLIISIFSLVASIGLIFYLYVIRDKSWSLKIAI
                     ALILGGAVGNLIDRAFYGLLFDYAPLFYGKVVDFFDVDFFDFTLFGRSYDRWPVFNIA
                     DAAVTIGVLILVLFYKKHQDEDEKLASALPDGTQISENNLAVTDQSTLAAKNENGTDL
                     MKEDSLNEQTDNRKEISD"
     gene            complement(1523..2284)
                     /locus_tag="VF870_05160"
     CDS             complement(1523..2284)
                     /locus_tag="VF870_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HEX9251606.1"
                     /translation="MATKKTVKKSPAKKGKSVVKKEQKKKKPVKAAPAKKTVKKQAPK
                     KKEVKKVTAKKVSKTKVNKIKAAPKKVVVAKKAEVQKKQVVKPQAKPKVVKKKEEEFI
                     TPEMTAANRAAALKKIKGYGKKELDHFREIILDKRDEILEQLQNLREQILDPTTGEYI
                     NENSPYSLHMAEQGTDAMEREKTFLYAQRENKFLGYLEDALKRIDAGTYGICVECIEE
                     PQHLCPTCPLIPKARLEAVPHSQLCLPMKQKQEKK"
     gene            complement(2307..>4972)
                     /gene="ileS"
                     /locus_tag="VF870_05165"
     CDS             complement(2307..>4972)
                     /gene="ileS"
                     /locus_tag="VF870_05165"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX9251607.1"
                     /translation="ALKTLFDKGLIYKDYKIVPQDPKSETVLSMAELALGYRETKDPS
                     VYVLFKLVNADEHFLVWTTTPWTLISNVALAVGSDIDYVKIKTEHATMILAKERLSVI
                     DKEYEILAEMKGSELAGIEYEQLMEYCDVDKKAFYVIEGDFVSTADGSGIVHIAPAFG
                     ADDYEVSKKYGLPMLQPVTRAGLFTDEVTDFAGQFVKDADAGIIVKLKHEGKLYRKET
                     ILHTYPFCWRHPEVPVIYYARESWFIRTTSIADRMVELNKQINWQPPEVGSGRFGNWL
                     EENKDWALSRDRFWATPLPLWVSEDGDVFAVGSIEEVKNGFIEENGKRVLLKDLKEID
                     LHKPFVDKILFEKNGKIYKRTPEVIDVWFDSGSMPFAQYHYPFENKEEFEKNYFPSQF
                     ICEGIDQTRGWFYTLHAISTMLFDNVAFKNVIVNELILDKTGRKMSKSVGNTVDPFLL
                     FDKYGADATRWYLVTNSPPWRPTLFDEEGLAEVQRKFFGTLVNTYSFFALYANIDGFN
                     FKDSVISYKDRPEIDRWIISKLNALVEEYEQLMNDYDVTKAARAVSNYTIDQLSNWYV
                     RRSRRRFWKSEMNKEKLSAYQTLYECLETICKLTAPFAPFISEAIYLDLNNETKREKF
                     ESVHLAEFPKTTYRDTVLEEKMEVAQNVVYLTRAMRAKNNLKVRQPLKKIMVALDKSK
                     HDALSKMKDVILEEVNIKELLILTDDSEIVNKSAKANFKSIGPKFGKKVKAVAEVIKN
                     FSKEEISKIEAGSEIVLSIQDENVIITKDDVEIISTEITGWVVEAEAGITVAIDAELD
                     AALIEEGLAREFVNRIQNMRKDAGFDVTDKIIINFTASENFVKAIKNFNQYISTETLA
                     EKLSEAKNTGIGFTQEFKIGEFDCSINIEKV"
BASE COUNT         1491 a         1015 c          731 g         1735 t
ORIGIN      
        1 ccataagtat gatcaccaaa aattggtttg tgcaacccgg aaagatgcac tcgtatttga
       61 tgtgttcttc ctgttttcaa attaagtttt atataagagg taaactcgta ctgctcaatc
      121 actttataaa aagtggttgc atcttttcct tctttatcac ttatagtaaa tttttttcta
      181 tcacttttac tgcgagtaat ttttgtactt atctcacctt ctttttcttt aaactttccc
      241 cagcagatcg cgtgatattc tctttcaata gtatgctttg aaaattgagc agccagcttt
      301 gcatgtgtaa aatcatcttt ggctacaaca agcaatccgc ttgtgtcttt atcaatgcga
      361 tgtacaattc ccggtcttcc accttcatta actgtgctta aagattttgt gtgatgaaga
      421 agtgcgttca ctaaagttcc ggtataattt gcataagccg gatgagcaac cattccagca
      481 ggtttattca cgataatgaa atactcgtct tcataaatta tgtccagcgg aatcgcctca
      541 gcttccacat cttcaggtct gggagaaatc agttgaactg cttcaacttt atcgaacggt
      601 tttacattgt aatttgattt aacaacaacg ccgtttactt taacaaatcc cgcatcaata
      661 gctttttgta ctttagatct tgtagtattt tcgatgtgat gagtaagaaa tgtatctatt
      721 cgttcttttc tttttccttc aggaacatca atcagaaatt tcttttcggt tatcagtttg
      781 ctcattgagc gaatcttctt tcattaaatc tgtgccgttt tcatttttag ctgctaaagt
      841 ggattgatct gtaacagcta aattattttc tgaaatttgt gtcccatcag gtaaagctga
      901 tgcaagcttt tcatcttcat cctgatgctt tttatagaaa agaacaagta ttaaaactcc
      961 aattgtaaca gccgcatctg caatattaaa aacgggccag cgatcataac ttcttccgaa
     1021 caaagtaaaa tcaaaaaaat caacatcaaa aaaatcaact acttttccgt aaaacaatgg
     1081 tgcataatca aacaacaaac cgtaaaaagc cctatcgatt aaatttccta ctgcaccgcc
     1141 aagtataagt gcaatagcga ttttaaggct ccagctttta tcacgaataa cataaagata
     1201 aaatatcaag ccaatacttg ctacaaggga aaaaatagaa atgattaatt taaagttgct
     1261 tcctggatcg tagccaaaag ccattccggg attttcaaca aatgtgagtc taaaaaaatc
     1321 tccaaatacc ggaatcattt gcccaagata cattccttca taattaatat tcaggaatgg
     1381 aatcgagaat cctttaacca tcaatttaga aacctgatca ataacaacaa cagcaaatga
     1441 taaatacaac gctttcaact aatacaaact ccttgatatg tatgcttaaa tttttccacc
     1501 aaattataat tagataataa tactatttct tttcttgttt ctgtttcatt ggtaaacata
     1561 attgactgtg aggaacagct tccaatcttg cctttggaat aagagggcaa gtcgggcaaa
     1621 gatgctgagg ttcttcaata cattcaacac agatcccata tgtaccggca tcaattcttt
     1681 ttaatgcatc ttcaagatat cctaaaaact tattttcacg ctgtgcgtaa agaaaagttt
     1741 tttctctttc catagcatct gttccctgct cggccatatg aagcgagtaa ggggaatttt
     1801 catttatata ttcgccagtt gtaggatcaa gtatttgttc tcttaaattc tgtaattgtt
     1861 caagaatctc atcacgttta tcaagaataa tttctctgaa gtgatcaagt tcttttttac
     1921 cataaccttt aattttcttt aaagctgcgg cacggtttgc tgctgtcatt tcaggggtaa
     1981 taaattcttc ttcttttttc ttgactactt tcggtttggc ttgtggtttt acaacctgtt
     2041 tcttttgaac ttcagctttt ttggcaacaa cgactttctt tggagctgcc ttaattttgt
     2101 ttaccttagt ttttgatact ttttttgctg tcactttttt cacttccttt ttcttaggtg
     2161 cttgtttctt tacagttttc tttgcaggtg cagctttaac aggttttttc tttttctgtt
     2221 cttttttcac aaccgatttt cctttttttg caggactttt tttaacagtt ttttttgtag
     2281 ccatgatgga tctcctctaa ttaggattat accttttcta tattaattga acaatcaaac
     2341 tcaccaattt taaattcctg agtaaatccg attcccgtat tttttgcctc agataatttt
     2401 tccgcaagag tttctgtgga aatatattga ttaaaattct taatagcttt aacaaaattt
     2461 tcagaggcag taaaattaat aataatttta tcagttacat caaaacctgc atcttttctc
     2521 atattctgaa ttcgattgac aaattcgcgt gctaaacctt cttcaataag cgctgcatca
     2581 agttctgcat caattgctac tgtaattccg gcttcagctt caacaaccca accggtaatt
     2641 tcagtactaa taatctcaac atcatccttt gtgatgataa cgttttcatc ctggatactc
     2701 aggacaattt cagaaccagc ttcaatcttt gaaatttctt ccttggaaaa gtttttaatt
     2761 acttctgcaa cggcttttac tttctttcca aactttggtc cgattgattt aaagtttgcc
     2821 ttagctgatt tattaacaat ctcagaatca tctgttaaaa taagcaactc ttttatgttt
     2881 acttcctcaa gtattacgtc cttcatttta gaaagagcat cgtgcttgct cttatccaaa
     2941 gcaaccataa ttttctttaa tggctgacgg actttaaggt tattctttgc acgcattgct
     3001 cgcgttaagt aaactacatt ctgtgcaact tccatttttt cttctaaaac cgtatctcga
     3061 taagttgtct ttggaaattc tgctagatga actgactcga atttttcccg tttagtttca
     3121 ttattcaaat caagataaat cgcctctgag ataaatggag caaatggcgc tgttaatttg
     3181 catattgttt ccaaacactc atacaaagtt tgataagcag aaagtttttc tttgttcatc
     3241 tcggatttcc agaaacgtct tctgcttctt ctaacatacc agtttgatag ctgatcaatt
     3301 gtgtaatttg aaactgcacg agcggctttt gtcacatcgt aatcattcat tagctgctcg
     3361 tactcttcaa caagagcatt caacttagaa attatccaac gatcaatttc aggtctgtct
     3421 ttataagaga taacagaatc tttaaaatta aatccatcta tgtttgcgta aagagcaaag
     3481 aaagaataag tgtttactaa agttccaaag aattttctct gtacttcagc taatccttct
     3541 tcatcaaaca atgtcgggcg ccacggcggc gagtttgtaa ccagatacca acgcgttgca
     3601 tctgcaccat atttatcaaa caacaaaaat ggatcgacag tatttccaac agatttagac
     3661 attttccttc cggttttgtc taagatcaat tcgttcacaa tcacattctt aaacgcaaca
     3721 ttatcaaaca gcattgttga aatagcgtgc aaagtataaa accatccgcg cgtttgatca
     3781 atcccttcac agataaactg agatggaaaa taattttttt caaactcttc tttattttca
     3841 aagggataat gatattgggc aaacggcatt gaacctgaat caaaccaaac atcaattact
     3901 tccggagtgc gtttataaat cttgccattc ttttcaaaaa gtattttatc aacaaatggt
     3961 ttgtgaagat caatctcttt taaatctttt aataaaactc tcttaccatt ttcttcaata
     4021 aatccgttct taacttcttc aatactgcca acagcaaaaa catcaccatc ttcacttacc
     4081 caaagcggta acggagttgc ccagaaacga tcccttgata acgcccaatc tttattttct
     4141 tcaagccagt taccaaatct tccacttcca acttccggcg gctgccagtt gatctgttta
     4201 ttcaattcaa ccattctatc tgcaatagaa gttgtgcgga taaaccatga ctcgcgtgca
     4261 taataaatta caggaacttc cggatgtctc cagcagaatg gatatgtgtg aagaattgtt
     4321 tctttcctat agagtttacc ttcgtgctta agtttaacaa taattcctgc gtctgcatct
     4381 tttacaaact gtccggcaaa atcagttact tcatctgtaa acaatccggc acgagtaaca
     4441 ggctgcagca tcggcaaacc atacttttta gaaacttcat agtcatcagc accaaatgcc
     4501 ggagcaatat gaacaattcc tgatccatca gcggtactta caaaatcacc ttcaattaca
     4561 taaaaagctt ttttatcaac atcgcaatat tccatcaact gttcatactc aattcctgca
     4621 agttcgctgc ccttcatctc agcaagaatt tcatactctt tatcaattac agacaatctt
     4681 tcttttgcaa gaatcatagt ggcgtgttct gttttgattt ttacataatc aatatcagaa
     4741 ccaacagcta acgcaacatt tgaaatcaaa gtccagggag ttgttgtcca aacaagaaaa
     4801 tgttcatcag cattcacaag tttaaacaaa acataaacag atggatcttt tgtttctctg
     4861 taaccgagtg caagttcagc cattgaaagc actgtttcag attttggatc ctgcggaaca
     4921 attttataat ctttatagat gagtccttta tcaaacaaag tttttagtgc cc
//
LOCUS       DASWBY010000279         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_311330, whole genome shotgun sequence.
ACCESSION   DASWBY010000279 DASWBY010000000
VERSION     DASWBY010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311330"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1032)
                     /locus_tag="VF870_05170"
     CDS             complement(166..1032)
                     /locus_tag="VF870_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251608.1"
                     /translation="MKNIILLLLFLGLYTPSLFSQTYSDDDDWYIDMNEELKAWENKR
                     PAISINFGISNLSHTDLEAAFADNNQIGLKLGYTYQRTKYADYIEKSTFNYLLINHNS
                     TELAGGSDAASDIETNNWQYGIAFSNGFGYKVGTEAVITPYYSSSISITDVKYLNDSL
                     SANDKYIMDRYEGSSRFSSSSEFGVRGQITDLLTIEAGYEIGAVFERWLFWKWAGSSI
                     IETAANNVLDIFIKEIFKSSPSAGPIAFIVLKGALGYGLYELRKEKMNWPFPSASPIL
                     FNNVKIGLTFNL"
     gene            complement(1284..1841)
                     /locus_tag="VF870_05175"
     CDS             complement(1284..1841)
                     /locus_tag="VF870_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251609.1"
                     /translation="MKSIKFAFIAVALLVSLIFSMPILAQGIELSDAVTINATVYGIE
                     KIDRTLWLKGPEGNVVEIDISEDAKNFNQIQIGDIVNITYYQSLAIYMGTPDEQPDLE
                     SDAMIVRAPEGEAPAGVAVEMVDVSATVLGIDKENRYVTLKGPAGDTFTTDVDESVSY
                     FDKLKVGDVIHVTYTKALAVDVELQ"
     gene            complement(1959..>2890)
                     /locus_tag="VF870_05180"
     CDS             complement(1959..>2890)
                     /locus_tag="VF870_05180"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9251610.1"
                     /translation="WNVGASKQAIIDFVKNSIDSTSKGFVPVADRIACFDNDGTLWTE
                     KPLPFQLYFVLDRIKAMAPQHPEWKTQQPYKGILEGDLKSAIAGGEKALFEMIMTTHA
                     GMTTDEFDKTVKDWMATATHPKTGKHYNEMVYQPMLELLNYLRANGYKTFIVSGGGID
                     FMRVWVEQSYGIPPYQVVGSSLKTKYDTTRTPPVFIKLAELNFNDDKLGKPVGIHQHI
                     GKRPVFTAGNSDGDYAMLQYTSTGSGPHFGMIVHHTDLMREYAYDRDDKLARLNNGLN
                     DAKKYGWIIVDIKTDWKKIYSFDEKTESDVTKD"
BASE COUNT          900 a          566 c          442 g          982 t
ORIGIN      
        1 cctgcgctga taatttgcct gttagaaaca atggaatacc gaataacagc aaatagtaaa
       61 atttaaaact cactgctaat cactaatttt aaacagttta ctatcataaa gatattccgc
      121 ggtaattaaa ttttatggat atttagatac agacacatta aatatttaca aattaaaagt
      181 tagacctatt tttacgttat taaacaaaat tggcgaagca ctaggaaaag gccaattcat
      241 tttttctttt cgtaattcat ataaaccata acccaaagca ccttttagaa caataaatgc
      301 aattggaccg gcagaggggg aagatttaaa gatttcttta ataaaaatgt ccaggacgtt
      361 attagctgca gtttcaataa ttgaactgcc cgcccatttc cagaataacc atctttcaaa
      421 tacagcacct atttcatatc cggcttcaat tgtcaacaaa tcggttattt gacctcgtac
      481 accaaactca gaacttgaac tgaaccggga agaaccctcg taacgatcca tgatatattt
      541 atcatttgcg ctaagtgaat cattcagata tttgacatca gtaatactta ttgatgagct
      601 gtaataagga gtgattacag cttctgttcc aaccttatac ccaaatccat tagaaaaggc
      661 aattccatac tgccaattgt tggtttcaat atcagatgct gcgtctgaac ctccggcaag
      721 ttcagtagaa ttatgattta ttagtaagta attaaaagtg ctcttttcaa tatagtctgc
      781 atatttagtt ctttgatatg tatagccaag ttttaaccca atttgattgt tatctgcgaa
      841 agctgcttct aaatccgtgt gtgaaagatt agaaatacca aagttaatag aaattgccgg
      901 tctcttattc tcccaggctt tcaattcttc attcatatcg atataccagt catcatcatc
      961 tgaataagtt tgtgaaaaca aggatggggt atataaacct aaaaataata atagtagaat
     1021 tatatttttc attttttgat ccttaaaaaa tattttttat aattaataat tttattactc
     1081 acttgtatta ctcgaaaatc tattcgatat attttaatac cacaatgaat tgaacaactt
     1141 taaattgttt accaagatat taagatagct gaataaatat taatctgggc gatgatacct
     1201 cctcaccctc tagttgaaaa aataaaacca tattttcact ttcagaagag gaggtactta
     1261 atttatttat tttatctatt gcattattgc aactcaacat ctacagccaa agccttggta
     1321 taagtaacat ggataacatc accaactttt agcttatcaa aatagctaac tgactcatca
     1381 acatcagttg taaatgtatc gcccgctggt ccctttaatg ttacgtaacg attctcttta
     1441 tcaataccta aaacagttgc agaaacatca accatttcaa ctgcaacacc ggctggtgct
     1501 tcaccttcag gagctcgaac aatcattgca tcggattcca aatctggttg ttcatccggg
     1561 gttcccatat aaattgctaa tgattggtaa taagtgatat ttacaatatc ccctatttga
     1621 atttgattga aattctttgc atcctcgcta atatctattt ctactacatt accttcagga
     1681 ccctttagcc agagtgtgcg gtctatcttt tcaataccat acaccgtagc attgattgta
     1741 actgcgtctg ataattcaat tccctgtgct aatatcggca tactgaatat taatgatact
     1801 aacagtgcga ctgctataaa tgcaaatttt atagacttca ttttattcct catttatttt
     1861 ttttgaaaat atttaattaa acaggtcata ttttttttag ctgatcgttt tttgaggcta
     1921 cctgaccgat aagacttttc gaacctgttt ccatgatttt agtcctttgt aacatctgat
     1981 tctgtttttt catcaaacga ataaatcttt ttccagtcag tctttatatc aactattatc
     2041 catccatatt ttttagcatc atttaaacca ttatttaacc ttgcaagttt gtcatccctg
     2101 tcataggcat attcacgcat taagtctgta tgatgaacaa tcattccaaa gtgagggcca
     2161 ctaccggtac tggtgtattg aagcatagca taatcaccat ctgaatttcc ggctgtaaat
     2221 acgggacgct taccgatgtg ctgatgaata ccaactggct taccaagctt atcatcattg
     2281 aaattaagtt cagcaagttt tataaaaacc ggcggtgttc ttgttgtatc atattttgtt
     2341 ttgagagaac ttcctactac ctggtatgga ggaatgccat aagattgttc aacccatact
     2401 ctcataaaat caataccacc acctgaaact ataaaagttt tgtaaccatt tgctctcagg
     2461 tagttcaata attccagcat aggttggtaa accatttcgt tataatgttt gccggtttta
     2521 ggatgcgtgg ctgttgccat ccagtctttc accgtcttat caaactcatc tgttgtcatg
     2581 ccagcatgag tagtcattat catttcaaac aaagcttttt caccaccagc tatggctgat
     2641 ttcaggtcgc cttccaaaat tcctttgtaa ggttgttggg ttttccactc aggatgctgc
     2701 ggtgccatcg ctttaattcg atcgaggaca aaatatagtt gaaaaggcaa tggcttttct
     2761 gtccataagg ttccgtcatt atcaaaacaa gcgattcgat ctgcaacggg aacaaaacct
     2821 ttactggtgc tgtcaatgct gttctttaca aagtctataa tggcttgctt tgatgcccct
     2881 acattccatg
//
LOCUS       DASWBY010000280         1786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_12351, whole genome shotgun sequence.
ACCESSION   DASWBY010000280 DASWBY010000000
VERSION     DASWBY010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12351"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF870_05185"
     CDS             <1..196
                     /locus_tag="VF870_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX9251611.1"
                     /translation="SGVLDSDAFGAFLENGLFDQATAESFRRNILEKSGSDDPMNLYI
                     KFRGREPKPDALIDKLGFND"
     gene            complement(288..1412)
                     /locus_tag="VF870_05190"
     CDS             complement(288..1412)
                     /locus_tag="VF870_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX9251612.1"
                     /translation="MEKVNSIIQTVKEKFAPDKRTAVFSIEPTESENKIIIKGETNLT
                     EAKSELEKKMKEAGLNFQDEIEILPSKKLGDKIYGVINLSVANFRSKPDHPAELVTQA
                     ILGTPVKVYKKGEDGYYLVQTPDGYISWLDDDGFAFMTEVQWNEWKSSPKIIYTKEYG
                     FSYSEADVNSQTVSDLVAGNLLKIMGEDSDFYLVNYPDGRVAYIKKDEAKLFNDWYNS
                     LNPTGETILKTAYRFMGIPYLWGGTSTKGMDCSGFTKTVYFLNGIILARDASQQVNTG
                     ELVDTKDGWTNLQAGDLLFFGRKASGDKKERITHVAIYIGDGDFIHAAGRVRINSFNK
                     SKTYYSQYRDNAFIRAKRILTSVGKNGIEKILDNNFYKSN"
     gene            complement(1577..>1786)
                     /locus_tag="VF870_05195"
     CDS             complement(1577..>1786)
                     /locus_tag="VF870_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251613.1"
                     /translation="IKFSLPERTFVELKIYNILGIEIATIEQKELDAGNYAYTFNAEN
                     LSSGIYLYKLNAGKFSITKKMTLIK"
BASE COUNT          556 a          311 c          283 g          636 t
ORIGIN      
        1 gtcgggagtt ttagacagtg atgctttcgg tgcattttta gaaaatggat tatttgatca
       61 ggcaacagca gaaagtttta gaagaaacat tttagaaaaa agcggaagtg atgatccaat
      121 gaatctttac atcaagttcc gtggtcgtga gcccaaacct gatgctctta tagataaatt
      181 gggatttaat gattgaagtt atagtaatga gaacaatttt actttgcaca ctttgcgtga
      241 aaagactcta agtcagattc acgcaaagct cgcaaagatt attaatttta attagattta
      301 taaaaattat tatctaatat tttttcaatt ccatttttac caacagaagt tagaattctt
      361 ttagctctga taaatgcatt atctctgtat tggctgtaat acgttttgct cttattaaaa
      421 ctatttatcc gtactcttcc ggcagcatga ataaaatctc catcaccgat gtaaatcgct
      481 acgtgtgtta ttctctcttt tttatcaccg ctagcttttc ttccgaagaa cagtagatcg
      541 ccggcttgta aatttgtcca gccatctttc gtatcaacta attcaccggt attaacttgc
      601 tgagatgcat cacgggctaa aattattcca ttcaagaaat aaacagtttt tgtaaaaccg
      661 ctgcagtcca ttccttttgt agaagtgccg ccccataaat atggaattcc cataaaacga
      721 taagcagttt ttaaaattgt ttctccggtt ggattaagtg aattgtacca atcattaaaa
      781 agttttgctt catctttttt aatgtaagca actcttccat ccggataatt aactaagtag
      841 aaatcagaat cttcacccat aatctttaat agatttccgg ccacaagatc agagacagtt
      901 tgggaattaa catcagcttc tgaataagaa aatccatatt cttttgtgta gataattttt
      961 ggcgatgatt tccattcatt ccactgaact tcggtcataa aagcaaatcc atcatcgtcc
     1021 aaccaggaaa tgtatccatc cggtgtttgt actaagtaat atccatcttc acctttttta
     1081 taaactttta ccggagtgcc taatatcgcc tgtgtaacaa gctcagccgg atgatctggt
     1141 tttgatcgaa agtttgcgac ggaaagatta attactccgt aaattttatc gcctaatttt
     1201 ttagaaggaa gaatttcaat ttcgtcttga aaatttaatc cggcttcttt catcttcttt
     1261 tccaattctg attttgcttc ggtaagatta gtttcgcctt tgatgataat tttattttct
     1321 gattcggtcg gctctatact gaagactgca gttcttttat ccggagcaaa tttttctttt
     1381 accgtttgaa tgattgaatt aactttttcc attttattaa tttcttgtga ataagtatga
     1441 aatgatatta ataccaatat tagaaagatc gctgaatgct tcattaatag tcctgattaa
     1501 aggtgaatga aaatctctgc ccaaatataa aaaaggcatc cataaaagga tgccttaaat
     1561 ttagaataaa aaaaatttat ttaatcagcg tcattttttt tgtaattgaa aatttacctg
     1621 cattaagctt ataaaggtaa attccactgg agagattttc agcattgaaa gtataagcat
     1681 aattacccgc atccagttct ttttgttcga tggtcgcaat ttcaattcca agtatattat
     1741 aaattttgag ttctacgaac gttctttcag gcaaactaaa tttaat
//
LOCUS       DASWBY010000281         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_46736, whole genome shotgun sequence.
ACCESSION   DASWBY010000281 DASWBY010000000
VERSION     DASWBY010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46736"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VF870_05200"
     CDS             <1..200
                     /locus_tag="VF870_05200"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX9251614.1"
                     /translation="NRPSFIEAARPEVAIPLYEKFIKRMKMNLGDKKIKTGIFGEMMQ
                     VKIMNDGPVTIIVESKKQVNK"
     gene            197..1102
                     /locus_tag="VF870_05205"
     CDS             197..1102
                     /locus_tag="VF870_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9251615.1"
                     /translation="MKPVLMIFIDGVGIGEKNYEINPFFKYGFRIFDELFKDTPYSQN
                     LFLEKNGMYVFPTDAKLGVEGLPQSGTGQVSIFCGINAPKFVGKHFGPFPYSTTIPII
                     EEKNIFRTFKELGKSAYFANAYPKVFFDYLRSGKTRLSTTSLSCRLAGIKLNSVTELR
                     QSKALTAEITNERWNRKLKYQLKVISPETAARRLLRIAGKNDFTLYEFFHTDHLGHGR
                     LTEEFESIYDNLNRFLFTILKELNKNDMTLIMCSDHGNFEDLSVKTHTINPALTITAG
                     YQAAQLAGSIKDLTDIKPSIIKYCT"
     gene            1093..>2336
                     /locus_tag="VF870_05210"
     CDS             1093..>2336
                     /locus_tag="VF870_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX9251616.1"
                     /translation="MHLKDYPFIKFTLAFIIGILASGIIQLQLFYFPIILFIILLTLL
                     FTKLFHLKFNKALSSLLLFMLVFVSGIFIAKQNEIKIQSPLLNYQKEKNAILYAKVIE
                     VELKRSFEIVFTVSIDSLLMDDKKFTSKDKLICKFRGDSLQRNILYDNLNAGNKIHLT
                     GTYQKGRERRNPGEFDYDKYLKSNGITGLFISYATDSINILSNQKDYFKSVLHSVRKK
                     IDKSIHDLHNAQTAGLLRGLLLADRSEIDYETKNEFINSGVVHILAVSGLHVGYVLVI
                     FIFIFGRFGIYLRSALTILGLLAFMFLTGVPPSVFRATLMAVIIIIAFLSNRSTNIIN
                     SISIAAFIILILNPNEIYNPGFQLSFAAVLAIGILYPYFQKMIYKFHLKHKWVENILL
                     FVGVSLSAQIGTLPFTLAYFSK"
BASE COUNT          813 a          352 c          391 g          780 t
ORIGIN      
        1 gtaatcgtcc aagttttata gaagctgcac ggcctgaggt tgcaattccg ctttatgaaa
       61 aatttattaa aagaatgaaa atgaatctag gtgataaaaa aattaaaacc ggaatatttg
      121 gtgaaatgat gcaggtgaaa attatgaatg atggtcctgt gacaattatt gttgaatcga
      181 agaaacaggt gaataagtga agcctgtgct gatgatattt attgatgggg ttggaatcgg
      241 tgaaaaaaat tatgaaatca atccattctt taaatatgga ttcagaatat ttgatgaatt
      301 gttcaaagat actccttact ctcaaaatct ttttttagaa aaaaatggaa tgtatgtttt
      361 cccgactgat gcaaaattgg gagttgaagg attaccccaa agtggaacgg gacaggtatc
      421 tatattttgt ggaataaatg ctccaaagtt tgttgggaag cattttggtc cttttccata
      481 ctcaacaact attcctatta ttgaagaaaa aaatattttc agaacattta aggaacttgg
      541 taagtcagcc tattttgcaa acgcatatcc aaaagtattt tttgattatt tacgatcagg
      601 aaagacaaga ttaagcacaa cttcattaag ctgcaggtta gcgggaataa aattaaactc
      661 agtaactgaa ttaaggcaga gcaaagcact tacagctgag attactaacg aaagatggaa
      721 tcgtaaatta aaatatcaac ttaaggttat aagtcctgaa actgctgcaa gaagattgtt
      781 aagaatcgcg ggcaaaaatg attttacatt atatgagttt tttcatacag atcatttagg
      841 gcatggtagg cttacggagg agtttgaatc gatttacgat aatcttaata gattcttgtt
      901 tacaatttta aaagagttaa acaaaaacga tatgacttta atcatgtgtt ccgatcacgg
      961 aaactttgaa gatctttctg taaaaactca tacaattaat ccggctttaa ctataaccgc
     1021 cggataccag gccgcgcaat tagccggcag tataaaagat ttaacagata ttaaaccatc
     1081 aatcataaaa tattgcactt gaaagattat ccttttataa aatttacctt agcatttatt
     1141 attggaattc ttgcttcagg aataatccaa cttcaactat tttacttccc aattatttta
     1201 ttcatcatcc ttctgacatt attattcaca aaactttttc atttaaaatt caataaagca
     1261 ctttcttctt tgctattatt tatgctggta tttgtttcag gaatatttat cgcaaagcaa
     1321 aatgaaataa aaattcaatc cccgcttctc aattatcaaa aagaaaaaaa tgcaatactt
     1381 tatgcgaagg ttattgaagt tgaacttaaa agatcttttg aaattgtttt tacagtctcg
     1441 atagattcac tattaatgga cgataagaaa tttacatcaa aagataaatt aatttgtaaa
     1501 ttcagaggtg attcacttca aagaaatatt ttgtacgata acctgaacgc aggtaataag
     1561 attcacttaa caggcactta tcaaaaggga agagagagaa gaaatcccgg tgaatttgat
     1621 tatgacaaat atcttaaatc aaatggtata accggattat ttatttctta cgcaacggat
     1681 agtatcaata tactttcaaa tcaaaaagat tattttaaaa gtgtgctcca ttctgttaga
     1741 aaaaagattg ataaatcaat acacgatctt cacaatgcac agaccgcagg attgttaaga
     1801 ggtctcttac ttgctgacag aagtgaaatt gattatgaaa ctaaaaatga atttattaat
     1861 tctggtgttg tgcatatcct ggctgtatca ggattacacg ttggatatgt gttggtaatt
     1921 ttcatattca tctttggaag atttggaatt tatcttcgct cggcacttac catccttggt
     1981 ttattggcct ttatgttttt aactggagta ccgccatcag tttttcgggc aacgttaatg
     2041 gcagtaatta ttattattgc attcctttca aatagaagta caaacattat taattccatt
     2101 tctatcgccg catttataat cctaatatta aatccaaatg aaatttataa tccagggttt
     2161 caactttcgt ttgctgcagt gctcgcaatc ggaattctgt atccttattt ccaaaaaatg
     2221 atttacaaat ttcacttaaa acataaatgg gtggaaaata ttttattatt tgtcggagtt
     2281 tcattaagtg cccaaatcgg aactttgcca tttacgcttg cgtactttag taagtt
//
LOCUS       DASWBY010000282         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_12366, whole genome shotgun sequence.
ACCESSION   DASWBY010000282 DASWBY010000000
VERSION     DASWBY010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12366"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VF870_05215"
     CDS             complement(<1..643)
                     /locus_tag="VF870_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9251617.1"
                     /translation="MGFSTDAIHAGQIPDPTTGAVITPIYQTSTYAQYELGKSTGFEY
                     ARTHNFTRQALEKNIAALEKGKYGIAFASGLAATHALMSLVKAGDHIVISNNVYGGTY
                     RLYELNMKNYGLDFTWVDSTDLKNVENAIRPNTKMIYVETPTNPMLNLTDLKSVSELA
                     KKNKLISVCDNTFMSPYFQNPLNFGIDIVLHSTTKYLNGHSDIIGGILVTSDEK"
     gene            complement(745..1128)
                     /locus_tag="VF870_05220"
     CDS             complement(745..1128)
                     /locus_tag="VF870_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9251618.1"
                     /translation="MYEKRNAKFDLEDRLIEFAVVIIKISENLSNTRAGNHIGGQLVR
                     SGTAPALLYGEAQSAESRNDFIHKLKILLKELRETLVALKIIKRVALTKTIESVDKSM
                     IECNELISIFVKSIETARKNNEKRK"
     gene            complement(1277..>1590)
                     /locus_tag="VF870_05225"
     CDS             complement(1277..>1590)
                     /locus_tag="VF870_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9251619.1"
                     /translation="TDFVKDVLQLISKTGYSQIPVMEGRQSIGCIRENRLLTKLVENP
                     LLYNSQIKDVMEESLPILDAKTEIEEVKKYLKTNSAILVSDFSLITDIITRYDLINIQ
                     A"
BASE COUNT          503 a          269 c          212 g          606 t
ORIGIN      
        1 tcttttcatc gcttgtaaca agaattccgc ctattatatc actatgtcca tttaaatatt
       61 ttgtagtact gtgaaggaca atatcaattc caaagttcaa aggattttga aaatagggac
      121 tcataaaagt gttatcacaa acagaaataa gtttattctt tttagccagt tcagagacag
      181 attttaaatc agtgagattt aacattggat tagttggagt ttcaacataa atcattttag
      241 tatttgggcg aattgcattt tcaacatttt taagatcagt tgaatcaacc cacgtaaaat
      301 ctaatccata atttttcata ttcaattcat acaatctata agttccgcca taaacattat
      361 ttgatataac gatgtggtcg cccgctttta ctaaactcat aagagcgtgc gttgcagcta
      421 agcctgaggc aaatgctatt ccatactttc ctttttcaag tgcggcaata tttttctcaa
      481 gtgcctgcct tgtaaagtta tgtgtacgag catattcaaa tcctgtgctt ttaccaagct
      541 cgtattgcgc ataagtagag gtttgataaa tcggagtaat aactgctcca gttgttggat
      601 caggaatttg tcctgcgtgt attgcatctg ttgaaaagcc catatcttta tattcttttg
      661 ttaattatta aagagtcgtt tagaatattg aacaccaaac atggaatgat gaatttcgaa
      721 ttaggaaaaa atattaaaca aattttattt tcttttttca ttgttctttc tagctgtctc
      781 aatacttttt acaaaaattg aaatcagttc attgcattca atcattgatt tgtcaactga
      841 ctcaattgtt ttagttaaag ccactctttt tataatttta agagcaacta aagtttctct
      901 aagttctttc agaaggattt taagtttatg aataaaatca tttcttgatt ctgcactctg
      961 agcttctcca tatagtaaag cgggtgctgt tccagatctc actaactgac ctccaatgtg
     1021 gttaccagca cgagtattac tcaaattttc tgaaatcttt attataacca ctgcaaattc
     1081 aattaatcta tcttcaagat caaatttagc atttcttttt tcatacatag tttattctca
     1141 agaaaaattt caaaagctct gatttaaatt tatttttatc attcaatatt tcttattaaa
     1201 taatcgatat taaaattaaa acttcatcaa tcaatattcc ttgttcatta ttcgatattc
     1261 tcttccatat attatatcac gcttgaatat taattaaatc atatcttgta atgatatccg
     1321 ttatcaaact aaaatcactt acaagaatag ctgagttagt ttttaaatat ttttttactt
     1381 cttcaatttc ggtttttgca tcaagaatcg gaagtgattc ttccattaca tcctttatct
     1441 gtgagttata tagcaaagga ttttcaacca actttgtaag caaacgattt tctctgatac
     1501 aacctataga ttgtcttcct tccataacag gaatttgtga ataacctgtt ttacttatca
     1561 gttgaagtac atcttttaca aaatccgttt
//
LOCUS       DASWBY010000283         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_12438, whole genome shotgun sequence.
ACCESSION   DASWBY010000283 DASWBY010000000
VERSION     DASWBY010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12438"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VF870_05230"
     CDS             <1..1314
                     /locus_tag="VF870_05230"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEX9251620.1"
                     /translation="DSGDLIFAVRGKYTNLFFNSSALISFKNESEENLIKFRQEFEAK
                     FFHNSFNNPDFDVIDGKSIGEIRKRYPFIGREIENEVIARKSDGENDSEILLKVLQDI
                     YTEDPAVYSDDYSGEVHIGFNSLKIFSSFEKQLFDKIINAFNQFLIKKYQYEEKNSKL
                     KKVNAFLDRELKKISSRLNNLLIVVEKGSQETEYNKIANLLLINLNNIQSGLSEIELE
                     DIYKPGSILKIKLDSKLSAKRNVDRYFERSHDSKINFEKSNKLYLNTKQEFEKLNSYK
                     QLLEKNPTLEDIDNIMKDLKIKDEEKQHSQDDLSSKFKHYLIDKKYHVFVGKDSVNND
                     LLTTRFAKQNDFWFHARSVSGSHVVLRIDNSKEVVPKNILKKAASLAAYHSKAKTAGV
                     VPVSYTLKKYVVKRKSQPTGQVSLLKEDVLLVKPEIPCDTDYLIE"
     gene            1440..3011
                     /locus_tag="VF870_05235"
     CDS             1440..3011
                     /locus_tag="VF870_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9251621.1"
                     /translation="MKKLIYLFVIIGLVAMSQQSYAQFKDWGTKFGIRGSILFPENEF
                     ANLGFSGNDNTSFDWFKMSYLGEAFFGFELSPALELSINGGYGKYAGVAMQAEGTDFG
                     EYETTIIPISLRFKVAPWDLKGWNPYFYVGGGVMNFSVDTPPDVKSPVTTEKDGWVAI
                     IPAGIGAEFALSERVLLDFALGGAMSSTYDLDSYRSGADDYWDSYFNASLGLTFTGEN
                     CKSDKDKDGLGKCDEEKIGTDPKNPDTDGDGLLDGEEYLTYKTDPLKTDTDADGLSDY
                     QEVKSTGTNPLIADTDGDGLSDGDEVNKYKTDPLVADTDKDGLNDSYEVVTSLTNPLK
                     ADTDGDGLTDGDEVLKYKTNPLVADTDGDGLTDSQEVLTYKTDPLKVDTDGGTVDDGT
                     EVKRGTDPLNPEDDVVKVGVVYSFEDIYFDVNKSTIRPESEARLNKVLQTLETYSDIS
                     VEISGHTDSDGSAKSNQKLSEARAESVKDWLVKKGIDAKRITTVGYGEDKPVADNKTK
                     EGKQKNRRIEFKRIK"
     gene            complement(3231..3545)
                     /locus_tag="VF870_05240"
     CDS             complement(3231..3545)
                     /locus_tag="VF870_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9251622.1"
                     /translation="MEANISDREDCTIIKFNKSKLLGTEAVEFQDAILNTLDKGINSI
                     VVDLSAVEYVTSWAIGMLVHAFTTSSNRNAKFTLVGVTQKVKEILSKVKLDKIFLIRD
                     TI"
     gene            complement(3626..>4836)
                     /locus_tag="VF870_05245"
     CDS             complement(3626..>4836)
                     /locus_tag="VF870_05245"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9251623.1"
                     /translation="SAAAVFKQLINLGIEPNRIYIAIIKDEKGFCDFWITDEDGSKVS
                     SGFEANLNDNSSFKKMFEGWKNKEKSMTIDMQGEELQEYFNHLTKLGVPFKGGLLQKR
                     RLQYIAYFSNGFIGVASPDQTKPETVKLLERFAAVFNLTYTRFNDLLQAEAQNRIIQA
                     ENERKSKELEDARQLQLSMLPKELPQLPHLDIAVYMKTATEVGGDYYDYIVDSDGTLT
                     FVVGDATGHGMMSGMMVSIMKSFFISNKSNLELKTFFENANNSIKDMQLGRLMMACVG
                     VQITPDKIIATNAGMPSLIYFRNKSQKAGEFVSHNFPLGGMKGTIYSLKEIKYEKGDI
                     LLLMSDGFAELKNDKNEQYGYPRITNEFKSVVHKSSNEIVDHLNKSASKWISGTEPDD
                     DITFVVIKVK"
BASE COUNT         1670 a          746 c          836 g         1584 t
ORIGIN      
        1 gattccggtg atttgatttt tgctgttcga ggtaaataca caaatctttt ttttaattca
       61 tctgctttga tttcattcaa gaatgaaagt gaagagaatt taattaagtt caggcaggaa
      121 tttgaagcaa agttttttca taacagtttc aataatccgg attttgatgt aattgatggg
      181 aaaagtattg gagagattag aaagagatat ccatttattg gccgtgaaat tgaaaatgag
      241 gtaattgcca gaaaatctga tggtgaaaat gattctgaaa tattattaaa agtgctgcaa
      301 gatatttata ctgaagatcc tgctgtttat tcagatgatt attcgggtga agtacatatt
      361 ggttttaatt cgcttaaaat attttcaagt tttgagaaac aattattcga taaaataatt
      421 aatgcattca accaattttt aattaagaaa tatcaatacg aagaaaaaaa tagcaaatta
      481 aaaaaagtaa atgcgttttt agatagagaa cttaagaaaa tttcatcacg actgaataac
      541 ctacttatcg ttgtggaaaa gggctcgcaa gaaacagagt ataataaaat tgcaaatctt
      601 cttttaataa atttgaataa tattcagtcc ggactatcag aaatagaact tgaggatatt
      661 tataaacctg gttccatatt aaaaataaag ttggatagta aactatccgc taaaaggaat
      721 gtggatagat attttgagag atcacacgac agtaaaatta attttgaaaa atccaataag
      781 ttatatttga acactaagca agaatttgaa aaattaaatt catataaaca attattagag
      841 aaaaatccaa ctttggagga tatagataat attatgaaag atcttaaaat aaaagatgaa
      901 gaaaaacaac atagccagga tgatctttct tcaaaattca agcattattt aattgataag
      961 aaatatcatg tgtttgttgg taaagattct gtaaataatg atttattaac tacaagattt
     1021 gcaaagcaaa atgatttctg gtttcatgca agatcggttt ccggttcgca tgttgtacta
     1081 agaatagata acagtaaaga agttgttcct aagaatattc ttaaaaaagc agcttcactt
     1141 gcagcctacc atagcaaagc aaagaccgca ggtgttgttc cggtgtcata cactttaaaa
     1201 aagtacgttg ttaagcgtaa atcacaacct accggtcagg tttctttact taaagaggat
     1261 gttttattag ttaagccgga aattccttgt gatacagatt acttaataga gtaaataatt
     1321 attgaaaata ttttgtcttt tattagatta gttctatctt ttgatcaaat taatagagag
     1381 tttagctcag tattagtaat attttaaagt acatatacca taaacaaatt gaggaaacaa
     1441 tgaaaaaact tatttacttg ttcgtaataa ttggcttagt tgcaatgagt caacaaagct
     1501 atgcacaatt taaagattgg ggtacaaaat tcggtatccg tggaagtata ttatttccag
     1561 aaaatgaatt tgcaaatctg ggattcagtg gaaatgataa cacatcattc gactggttta
     1621 aaatgtccta cttaggtgaa gctttctttg gttttgaatt atcacctgca ttagaattat
     1681 ctattaatgg tggttacgga aaatatgccg gcgttgcaat gcaagctgaa ggtactgatt
     1741 tcggtgaata tgaaacgaca attattccga tctccttaag atttaaagtt gcaccctggg
     1801 acttaaaagg atggaatcca tacttttatg ttggtggcgg agttatgaat tttagcgttg
     1861 atacaccacc tgatgttaaa tcgcctgtaa ctacagaaaa agatggctgg gtagcaatta
     1921 tccctgcagg tataggtgct gaatttgcac ttagtgaaag agtactttta gattttgcac
     1981 ttggtggcgc tatgtcatct acatatgacc tggattcata tagaagcggt gcagatgatt
     2041 attgggattc ttattttaat gcatcgttag gcttaacttt cactggtgaa aactgtaaat
     2101 ctgataaaga taaagacggt ttaggaaaat gtgatgaaga gaagatcggg accgatccaa
     2161 aaaatcctga tacagacggc gatggtttat tggatggaga agaatattta acttataaaa
     2221 ctgatccatt aaaaaccgat actgatgcag atggtttatc agattatcag gaagttaaat
     2281 ccacaggaac caatccattg attgctgata ctgacggtga tggattatca gatggtgatg
     2341 aagttaacaa gtacaaaaca gatccactgg ttgctgatac agataaagac ggattaaacg
     2401 acagttatga agttgttaca agcttaacaa atccattaaa agctgataca gatggcgatg
     2461 gattaactga tggtgatgag gttcttaaat acaagactaa tccattagtt gctgatactg
     2521 atggtgatgg attaactgat tcacaagaag ttctgacata taagaccgat cctcttaaag
     2581 ttgatacaga cggaggaact gttgatgatg gtacagaagt taaacgtggt acagatccat
     2641 taaatcctga agatgatgta gttaaagtcg gtgttgttta ttcatttgaa gatatttact
     2701 tcgatgtgaa taaatccaca atcagaccag aatcagaagc aagattaaat aaagttctac
     2761 aaactttaga gacttattca gatatttctg ttgaaataag cggacatact gatagtgatg
     2821 gtagtgcaaa gagcaatcaa aaactttctg aagccagagc tgaatctgta aaagattggt
     2881 tagttaaaaa aggcatcgat gctaagagaa taactactgt tggttatggc gaagacaaac
     2941 cagtagcaga taacaagaca aaagaaggaa aacaaaagaa cagaagaatt gagttcaaga
     3001 gaataaaata attctggtct taattagaat taagggtgtc caattggaca cccttttttt
     3061 atttatatct gccaatttta tttaatgccg tgctaaatat aaaatgatta tttttagtgc
     3121 ttattgatga taattttaag aaatgactag attagaaaaa agcacataag ttctctcgga
     3181 agaagctccc cttctacaaa cgaagtaaat tattttcgat tcttataaat ttatatggta
     3241 tcacgaatca aaaatatttt atcaagtttt acctttgata aaatttcttt cactttttga
     3301 gtgacgccga ccaaagtaaa ttttgcatta cggttacttg aggttgtaaa tgcgtgaaca
     3361 agcattccta tcgcccagct tgtaacatat tctactgcag aaaggtccac tacaatagaa
     3421 tttattccct tgtctagagt attaagaata gcatcctgaa actctactgc ctcggtgcct
     3481 aaaagtttac ttttgttaaa ttttataatt gtacaatctt ctcggtcact tatatttgct
     3541 tccacaatat accctttcaa gtttgtatta tttttatatg ataattacaa aaatctttta
     3601 aacaataaaa atgaataaat aataattatt taaccttaat tacaacaaag gtaatatcat
     3661 catcgggttc tgttccgcta atccacttgg atgctgattt atttaaatga tcaactattt
     3721 cgtttgaact tttatgaact accgatttaa attcatttgt tattcgtgga tacccatatt
     3781 gctcattctt atcattcttc aattctgcaa atccatcact cattaacaat agtatatcgc
     3841 ctttttcata tttaatttcc ttcagcgaat atatagtgcc cttcatccct cccaaaggaa
     3901 aattatgact aacaaactca cctgccttct gagatttatt tctaaagtaa attaatgatg
     3961 gcattcctgc atttgtagca attatcttat ctggtgtaat ttgaactccc acacaggcca
     4021 tcattaatct tcccaactgc atatctttta ttgagttatt ggcgttctca aaaaacgtct
     4081 ttaattctaa attggattta tttgaaatga aaaatgattt cataattgaa accatcatac
     4141 cggacatcat tccatgtcct gttgcatctc cgacaacaaa agtaagggtt ccatctgaat
     4201 caacaatata atcatagtaa tcaccaccga cttctgtagc agttttcata taaacggcaa
     4261 tatcaagatg cggcagttga ggcagttctt ttggaagcat agatagctga agctgacgtg
     4321 catcttcaag ttctttggat ttacgctcat tttccgcctg aataattcta ttctgcgctt
     4381 ctgcttgtag taaatcatta aaacgagtat atgttaaatt aaaaactgct gcaaaacgtt
     4441 ccagcagctt aactgtttct ggtttcgttt ggtctggcga tgcaacaccg ataaatccat
     4501 tagaaaaata tgcgatgtat tgcagcctgc gcttttgtaa aagcccgccc ttgaaaggta
     4561 cccctagttt tgtaagatga ttaaaatatt cttgtaactc ctctccctgc atatcgatag
     4621 tcatcgattt ttctttgttc ttccatcctt caaacatttt tttaaatgaa ctgttatcat
     4681 ttaagtttgc ttcaaaacca gagcttacct tacttccatc ttcatctgtt atccagaaat
     4741 cacagaagcc cttttcatct tttataatag cgatataaat tcgattcggt tcgataccaa
     4801 gatttattaa ttgcttaaaa accgcagcag cagaat
//
LOCUS       DASWBY010000284         4724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_84865, whole genome shotgun sequence.
ACCESSION   DASWBY010000284 DASWBY010000000
VERSION     DASWBY010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84865"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /gene="arcC"
                     /locus_tag="VF870_05250"
     CDS             <1..925
                     /gene="arcC"
                     /locus_tag="VF870_05250"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX9251624.1"
                     /translation="LGGNALLRGNEIGTIQQQEKNTYDTCISLLNLLKENYNLVITHG
                     NGPQVGNIMLRNEAGYNNYKIPKMPLDICVADSQGGIGYMIERQVYNIFKDQKIKKNV
                     VTLVTQVLVDKNDPAFENPTKPVGAFYLKEEADLLARVNKFVFKEDPRKRGWRRVVPS
                     PQPIDILNKKVIKDLVNKGNLVIASGGGGVPVYRGEDKKLYGVEAVIDKDHASALLAQ
                     EIGAEAFYILTDVPKVYLRFHKPDQQALNVITADEAQKYYDEGEFAAGSMGPKILAAI
                     KFVKGGGKETVITDSTMLGNPEGGTRIVSTR"
     gene            1025..1957
                     /gene="argF"
                     /locus_tag="VF870_05255"
     CDS             1025..1957
                     /gene="argF"
                     /locus_tag="VF870_05255"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX9251625.1"
                     /translation="MAVNMKGKDLISIADLTLEEIYEIFDVSKSLKEKKYTGEPHHLL
                     EGKTLGMIFTKRSTRTRVSFEVGIYQLGGIGLYFGPNDLQLGTSESIEDTAKVLARYL
                     DGIMIRTFAHSDVTNLAKYANIPVINGLTDLLHPCQVLTDLFTILEKKRNLKGLKLAY
                     IGDGNNMAHSLLNGCSKVGMNISIASPSGYKPDKEIVENAKKFAKYMGSKVEILEDPV
                     AAVKNADIVYTDVWASMGQESEAAERRKKFMKYQVNPKLVKNAKDDYLFMHCLPAHRG
                     DEVVNEVADSVNSVIFDEAENRLHVQKAIMALVM"
     gene            complement(2011..2493)
                     /locus_tag="VF870_05260"
     CDS             complement(2011..2493)
                     /locus_tag="VF870_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX9251626.1"
                     /translation="MPNITSIVEIIQLMLAPGIMISACGLLLLGMNNKYSLVVNRIRL
                     LNEERRKAIHKTTEDKTFNYQETQRLESISMQITSLVYRVKLVRNAVLAYTTAVALFV
                     LTSLSIGFGFLFEITRLNSFITTLFLLGMISVLCGVIFAAYETYKGYEIINFEVKIDE
                     "
     gene            complement(2512..2937)
                     /locus_tag="VF870_05265"
     CDS             complement(2512..2937)
                     /locus_tag="VF870_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9251627.1"
                     /translation="MESIQDILKGREIFTVHSGSSIKETVNFMASKGVGLVPVMKDEK
                     LIGVFSERDLVKRVISKDKDLTSTLIDDVMSTSLVIAKIDESNESVLAKMKEAKTRHI
                     LIIDNEKLVGVLSLRDLLEIDLNHCKTTVEVLNNYIYSK"
     gene            complement(3060..3791)
                     /locus_tag="VF870_05270"
     CDS             complement(3060..3791)
                     /locus_tag="VF870_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9251628.1"
                     /translation="MIEIKDLYKSFGNKKVLQGVNLTIEQGETIVIIGRSGCGKSVLI
                     KHIVGLLSPDSGYVKVENKIVNDLSQNELYELRKKFGFLFQGAALFDSMTVEENVGLP
                     LVESRTEFSKNEIKKIVEEKLSLVGLEEILNLKPSELSGGMKKRVALARALVTNPSYI
                     LYDEPTTGLDPIMSDSIDQMIKDLSEKLNVTSVVVTHDMYSVKNVANRVAMMNDGILY
                     FAGKPDELINSTDKVIEDFIRRTEV"
     gene            complement(3925..4614)
                     /locus_tag="VF870_05275"
     CDS             complement(3925..4614)
                     /locus_tag="VF870_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX9251629.1"
                     /translation="MFARKYLSLFLMLVLGITVFTGCSDDEQVVAPMPSTSKVLVTHA
                     SPDAPGVDILVDNTVAATNLTFPNSVGYAELTAGTRNIKVNVTGTSTTALQANLPLEA
                     NKNYSIFAVNTVSSIEAIVLEDNLTAPASGKAHVRFIHLSPDAPAVDITTTSGAVVFG
                     NYTFKQASAFTPLDAGTYDLQVRLAGTSTVVLDLPGIALAPGKIYTVFAKGLVSGTGV
                     QALGAQIIVNN"
BASE COUNT         1605 a          786 c          836 g         1497 t
ORIGIN      
        1 acttggagga aacgcgcttc ttaggggaaa tgaaatcggc actatccagc aacaggaaaa
       61 aaacacatac gatacgtgta ttagtctttt aaatcttctt aaagaaaatt acaatcttgt
      121 aatcactcac ggtaacggtc ctcaggtggg taacattatg ctgcgtaatg aagcaggata
      181 caacaattac aaaattccta aaatgccgct tgatatttgc gtggctgatt cacaaggcgg
      241 gattggttat atgattgaaa gacaagttta caatattttc aaagatcaga agatcaaaaa
      301 aaatgttgtt actcttgtta cgcaagtatt ggttgataaa aatgatcctg catttgaaaa
      361 tccaaccaaa cctgttggtg cgttttattt gaaggaggaa gcagatcttc ttgcaagagt
      421 aaataagttt gtcttcaaag aagatccaag gaaacgaggc tggagaagag tagttccatc
      481 accacagcca atcgatatct taaataaaaa agtaattaaa gatttagtta ataaaggaaa
      541 tttggttata gcttccggcg gaggcggtgt tccagtatat cgcggtgaag ataaaaaatt
      601 atatggagtc gaagctgtaa ttgataagga tcatgcatca gctttgcttg ctcaggaaat
      661 tggtgctgaa gcgttttata ttcttacaga tgtacctaaa gtttatttaa ggtttcataa
      721 acctgatcaa caagctttga atgtaattac ggcggatgag gctcaaaaat attatgatga
      781 aggcgagttt gctgcaggaa gtatgggacc aaaaattctt gctgcaataa aatttgtaaa
      841 gggtggcgga aaagaaacag ttattaccga ttcaacaatg cttggcaatc ctgaaggtgg
      901 aacaagaata gttagcacaa gatagtttaa caattgagat tttattaaat ttttcttgaa
      961 attgaaactg aacttagact tgaactttaa tcgaacttaa actttaaaat tacaggagtt
     1021 atttatggca gttaatatga aaggcaaaga tttaatttct attgctgatc ttacacttga
     1081 agaaatttat gagatttttg atgtaagtaa atctcttaaa gaaaaaaaat acaccggcga
     1141 gccgcatcat ttacttgaag gtaaaacact tgggatgatc tttacaaaaa gatcaacaag
     1201 aacaagagtt tcttttgaag ttggtattta tcagcttggc ggtattggat tatactttgg
     1261 cccgaatgat ttacagcttg gaaccagcga aagtattgaa gatacagcaa aagttcttgc
     1321 aagatattta gatggaatta tgattcgtac ctttgcacac agcgatgtta ctaatcttgc
     1381 aaagtatgca aacattccgg ttataaatgg attgactgat ctgcttcatc cctgccaggt
     1441 tttaacagac ttgtttacga tacttgaaaa gaaaagaaat ttaaaaggat taaaacttgc
     1501 gtacataggt gatgggaaca atatggctca tagtctatta aacggctgtt caaaagttgg
     1561 aatgaacatt tcaattgcat cgccatcagg ttataaaccg gataaagaaa ttgttgagaa
     1621 tgccaaaaag tttgcaaaat atatgggcag taaagttgag atacttgaag atccggttgc
     1681 tgcagttaag aatgcagata ttgtttatac agatgtttgg gctagcatgg gtcaggaatc
     1741 tgaagctgct gaacgcagaa agaaatttat gaaataccaa gtaaatccaa agttagttaa
     1801 aaatgcaaaa gatgattatt tattcatgca ctgcttgccg gcacaccgcg gtgatgaagt
     1861 tgtaaatgaa gttgctgatt cggttaactc agtaatattt gatgaggctg aaaaccgatt
     1921 gcatgttcag aaggcaataa tggctttggt tatgtaattt actcagcgtc tctgcggtaa
     1981 aaaactcttc taaccgcaga ggcgctgaaa ttattcatca atcttcactt caaaattaat
     2041 tatctcatat cctttgtaag tttcataggc tgcaaaaatt actccgcata aaacagaaat
     2101 cataccgagt agaaaaagag tagtgataaa tgaattaagt ctggtaatct caaataaaaa
     2161 cccaaatcca attgaaaggg atgtaagaac gaataaagca acagcagtcg tataagctag
     2221 tacagcattt ctaacaagtt taactctgta aactaggctg gtaatctgca tagaaatact
     2281 ttccagcctt tgggtttcct gataattaaa ggttttatct tctgttgttt tatgaatcgc
     2341 ttttcttcgc tcttcattga gcaaacggat cctgtttaca acaagtgagt atttattatt
     2401 cattccaagt aataaaagtc cgcacgcaga aatcataatt cccggagcga gcataagttg
     2461 aattatttcg actatagagg taatgttggg catatttata aataattgtt tttacttgga
     2521 gtaaatataa ttatttaaaa cttcaacagt tgttttacaa tgattaagat caatttctaa
     2581 gagatcgcgg agagaaagga caccaacaag tttttcgtta tcaataatta aaatgtgtct
     2641 agtcttggct tccttcatct ttgcaagaac agattcattt gattcatcaa tttttgcaat
     2701 aacaagactt gtgctcataa catcatcaat aagggttgaa gtaagatctt tatcttttga
     2761 tattactctt ttaaccaaat cacgctcaga aaaaacgccg atcaattttt catccttcat
     2821 tacagggaca agaccaactc cctttgaagc cataaagttt acggtttctt ttattgatga
     2881 accggaatgg accgtaaaaa tttcacgtcc ctttaatata tcttgtattg attccatatt
     2941 taagtccttt aattattttt tgaaatataa tctttttatt aagaagtgca aagagatata
     3001 aaaacaaaac aattccaaaa gctttttttg attgattgtt taataaatag aagatagcac
     3061 tatacttcag ttcttctaat aaagtcttca ataactttat ctgtagaatt aattagctca
     3121 tcgggtttgc cggcaaagta aagaattcca tcgttcatca tagcaacgcg attagctacg
     3181 tttttaacgc tgtacatatc gtgtgtaact acaaccgagg ttacatttaa tttttctgaa
     3241 aggtccttta tcatttgatc gatagagtca gacattatgg gatcaagtcc agtcgtgggt
     3301 tcatcatata aaatatagga aggatttgta accagtgctc ttgcaagagc aacacgtttt
     3361 ttcattcctc ctgaaagttc agatggtttt aaatttaata tttcttcaag tccaaccagt
     3421 gataatttct cttcaacaat ttttttaatt tcatttttag aaaactcagt tcttgattcg
     3481 actaatggta atcccacatt ttcttcaaca gtcatagaat caaacaaagc cgcaccctga
     3541 aataagaacc caaacttttt tctcaactca taaagttcat tttgagaaag atcatttaca
     3601 attttatttt caactttaac gtaacccgaa tcaggactaa gcaaaccaac aatatgcttg
     3661 attaaaacac ttttgccgca gccgcttctt ccaattatta cgatagtttc gccttgctca
     3721 attgtaagat taacaccctg taaaactttt ttgtttccga aacttttata tagatctttt
     3781 atttcaatca tattaaataa atttattgtt gatcaaaaat aatgaaaaag atttactaat
     3841 agctctgaaa gaaatttaaa aataaaaaag ccggattttt tcaaacccgg ctttcaccat
     3901 catcatctaa tcgctaccta atcattagtt attcacgatt atctgagctc ctaaagcctg
     3961 tacaccggta ccgctaacta aacctttagc aaaaactgta tagattttac caggtgcaag
     4021 tgctatcccg ggtaaatcta aaactacggt tgaagttcca gccaatctga cttgcaaatc
     4081 gtaggttcct gcatcaagcg gagtaaatgc agaagcttgc ttaaatgtat aattaccaaa
     4141 taccacagca ccagaggttg ttgtaatatc tacagcaggt gcatcaggtg ataagtgtat
     4201 aaatctaacg tgagctttac cgcttgcagg tgcagtaaga ttatcttcca gtacgattgc
     4261 ttcaattgaa gaaacagtat taactgcaaa aatcgagtaa ttcttatttg cttctaaagg
     4321 taaattagcc tgcaaagcag tagttgatgt accagttacg ttaactttga tatttcttgt
     4381 acctgcagtt aactctgcat atccaacact attaggaaat gtaagatttg ttgcagctac
     4441 tgtgttatct accaaaatat caactcctgg cgcatctggt gaagcatgag taacaagaac
     4501 tttagaagtt gagggcatag gtgctactac ttgttcatca tcgctgcatc ctgtaaatac
     4561 agttatacct aacactaaca ttaaaaacaa agaaagatat tttcttgcga acatttgaac
     4621 ctcctgatat attttattat gatttattta caatgtctaa tgggataaca gaagtatatg
     4681 taaatgagtt cccaaagttt taggtcattt atttacccct ttaa
//
LOCUS       DASWBY010000285         4122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_422986, whole genome shotgun sequence.
ACCESSION   DASWBY010000285 DASWBY010000000
VERSION     DASWBY010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422986"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VF870_05280"
     CDS             <1..321
                     /locus_tag="VF870_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9251630.1"
                     /translation="GQKQLISFARALAYDPKILILDEATSSIDTETEQLIQSAIEKLL
                     IGRTAIVIAHRLSTIQNADKIIVMHKGEIREIGNHQELLAKRGIYYKLYQLQYKDQEL
                     KKSS"
     gene            348..1373
                     /gene="fbp"
                     /locus_tag="VF870_05285"
     CDS             348..1373
                     /gene="fbp"
                     /locus_tag="VF870_05285"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX9251631.1"
                     /translation="MERIKFNTLARHIYEEERKYPEATGELSDLLHDLSLAAKVISLE
                     VNKAGLVDIIGFTGEENVHGEKVKKLDVFANDMMIKAMDHGGHLCVMASEEEEDIIHI
                     PPEFYLGKYVLLFDPLDGSSNIDANVSIGTIFSIYRRVSPEGGPGTMEDCLQPGIKQV
                     AAGYIIYGSSTIFVYTAGNGVHGFTLDPSFGEFILSHPDIKTPKKSKIYSINEGNYLY
                     WHPGLKKYIKWLQDEDKTTDRPYSSRYIGSMVSDIHRNLLYGGIYMYPADSRNPNGKL
                     RLMYECNPMAFIVEQAGGRATNGKQRMLEIKPASLHQRIPIFIGSEEDVLLVEKFMRD
                     YDPIEVK"
     gene            complement(1526..1789)
                     /locus_tag="VF870_05290"
     CDS             complement(1526..1789)
                     /locus_tag="VF870_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251632.1"
                     /translation="MTTLPPPKVKKAPEDSLESKVYSMVETFKEFIPIPNDRNRLSYC
                     LLKYLNGEGDAPKILLKTSKINYEGISKEELAKKLDDELSKIK"
     gene            1911..2912
                     /gene="waaF"
                     /locus_tag="VF870_05295"
     CDS             1911..2912
                     /gene="waaF"
                     /locus_tag="VF870_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX9251633.1"
                     /translation="MTVEKLSKLNRLLIIRLSSLGDILLTTPFIRSLKMQFPNIIIDM
                     LIREEYADVVKLNPYIDENFLYSKTAEDSQSLIKDLNIKNYDLVIDLQNNLRSKKIIS
                     LLNIKNLKFNKRSFDKFLLVNFKINKLKEAPQIPVRYSNTIQNIKLDEHGLDLFTDKK
                     ANTKIIGKNNLIGFCPGAKHFTKRWPKEYFIDLGNKLAQNGYNIVLFGGKIDKDICEE
                     LHKIISNSINLSNDDDLLQTTADMKLCKAVLCNDSGLMHTASAVGTKVIAVFGSTVKE
                     FGFTPYNCDHLILENNLLTCRPCSHIGRSDCPKKHFNCMKSIKPEFVFEQLKNYLNN"
     gene            2912..>4122
                     /locus_tag="VF870_05300"
     CDS             2912..>4122
                     /locus_tag="VF870_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9251634.1"
                     /translation="MNLLFNIFYNFLVLPFLYAVLHSAGLFNKKIRTGIIGRKRVYEE
                     LILSATSIDKNKKLIWFHSSSLGEFEQAKPIIERLKQEKDVNVLITFFSPSGYVNSKK
                     YPHADLISYIPFDTKSNAEKFIAITNPTLSVIMRYDVWPNMITSLNNKNIPVYLVDAT
                     LRDNSPRLLPLIKSFHKILFGKFSRILTVSDSDAEQFKSFGFDDKKVSAVGDTRFDRV
                     YQRSLLAKEKRLISSNVLKDKKILVAGSTWEQDEEVIFPAFLKLANVDKSVLMIVAPH
                     EPTLLHLEKIENEFAGKLKTIRFSHLNNYSDERIIIVDSIGILLTLYTYANVAYVGGS
                     FKQNIHNVLEAAVYGTPVLFGPKIENSQEATKLAEIGGGIIINNKRQAYRNLRRLFEN
                     ENLRTTLGNISA"
BASE COUNT         1447 a          660 c          708 g         1307 t
ORIGIN      
        1 ggacaaaaac aattaatttc ttttgcaaga gctttagctt atgatccaaa aatcttaatt
       61 ttggacgaag ctacttcaag tatagataca gaaacagaac agctgattca gtctgctatt
      121 gaaaaacttc ttatcggcag aactgcaatt gtaattgcac acaggctttc aactatacag
      181 aatgcagata aaattattgt tatgcacaaa ggcgaaatca gagagattgg taatcatcag
      241 gagctgcttg caaaacgagg tatttattac aagctatatc aattgcagta caaagatcag
      301 gaactaaaaa aatccagcta acagaatttt agaatgggag atttgctatg gaaagaataa
      361 aattcaatac tcttgcccgc catatttacg aagaagaaag aaaatatccc gaagcaactg
      421 gcgaattatc agatcttctt cacgatttat ctcttgcggc aaaagttatt tcacttgagg
      481 ttaataaagc aggcctggtt gatattatcg gttttaccgg tgaagaaaat gttcatggtg
      541 aaaaagtaaa gaagctggat gtttttgcaa atgatatgat gattaaagcg atggatcatg
      601 gcggtcatct ttgtgttatg gcttctgaag aagaagaaga tattattcat attccacctg
      661 aattttattt agggaaatac gtacttttat tcgatcccct tgatggttca tcgaacatag
      721 atgccaacgt aagtatagga actatctttt ccatttacag aagagtttcc ccggaaggcg
      781 gaccgggaac tatggaagat tgtttacaac caggaataaa gcaggttgca gccggataca
      841 ttatttacgg ttccagtaca atctttgttt atacggcggg gaatggtgta catggtttta
      901 cactcgatcc atcttttggc gaatttatct tatcacatcc cgatattaaa actcctaaga
      961 aatcaaaaat ctacagcatt aatgagggta actatttata ctggcatccc ggtttaaaaa
     1021 aatacatcaa gtggctgcaa gatgaggata agacaactga cagaccatat tcttcgcgtt
     1081 atataggatc aatggtttcg gatattcacc gaaatcttct ttacgggggg atatatatgt
     1141 atcctgcaga ttcaagaaat cccaatggta aattaagatt gatgtatgag tgtaacccga
     1201 tggcgtttat cgttgaacaa gccggaggca gagcaactaa tggaaaacaa aggatgttgg
     1261 aaattaaacc agcttcattg caccagcgga ttccaatttt tattggaagc gaagaagatg
     1321 tactgcttgt agaaaagttt atgcgggatt atgatccgat tgaggtaaaa taaaaatggt
     1381 ctctcccaat aggccgttta ataaatcgat atttatctgt tattcccatg caaacgggaa
     1441 tccataaaga taaaacaaat tattttccca ctttcatggg aatgaaaatc atatattttt
     1501 tgcttttgaa acagcttttt ttgatttatt taatcttact taattcatca tcaagttttt
     1561 ttgccagttc ttctttcgaa attccctcat aatttatctt ggaagttttt aataaaatct
     1621 tcggagcatc accttctcca tttaaatatt ttaagaggca ataggatagt ctgttcctgt
     1681 cattgggaat tggaataaac tccttaaaag tttcaaccat agaataaact tttgattcaa
     1741 gtgaatcttc cggcgctttt tttacttttg gaggaggcaa agttgtcata aatcagtcct
     1801 tattaaattt tatgccgtaa aattaggcac taaatcaaaa agttcaaatc attttgtgga
     1861 tataactttt ctaaatgata aatttgcaga tagattaaac aaagatttac ttgacagtcg
     1921 aaaaattatc aaaacttaac agacttctta tcatcaggct tagctcgctt ggggatatac
     1981 ttcttactac gccatttatt cgctctctaa aaatgcaatt cccgaacatt ataattgata
     2041 tgttgataag agaagaatat gctgatgtgg tgaaactgaa tccttacatt gatgaaaatt
     2101 ttctatatag taaaacagca gaagacagcc aaagcttaat caaagattta aatattaaga
     2161 attatgatct agtaattgat ttgcagaata atcttcgttc taaaaaaata atttctttat
     2221 taaatattaa aaatttaaaa tttaataaaa gaagttttga caaattttta ttagttaatt
     2281 ttaagatcaa taaacttaaa gaagctcctc agattcctgt tagatattca aatacaatcc
     2341 aaaacataaa actagatgaa cacggacttg atcttttcac agataaaaaa gcaaatacaa
     2401 aaataattgg gaaaaataac ttaattggat tttgtcccgg tgcaaaacac tttaccaaac
     2461 gatggccaaa agaatatttt attgatcttg gcaataaact agcgcaaaac ggttataata
     2521 tagttttatt cggcggcaag attgataaag atatttgcga agaattacat aaaattattt
     2581 ctaactcaat caatctttca aatgatgatg atttattaca gaccacagca gacatgaaat
     2641 tatgcaaagc cgtgttatgt aatgattcag gattaatgca cactgcttct gcagttggaa
     2701 caaaagtaat tgctgttttt ggttcaacag ttaaagaatt tggatttaca ccctataact
     2761 gcgatcactt aattttagaa aacaatttgc taacttgccg tccgtgttcg cacattggca
     2821 gaagtgattg tcctaaaaaa cattttaatt gtatgaaatc aattaaacca gaatttgttt
     2881 ttgaacaact gaaaaactat ttaaacaatt aatgaattta ctttttaaca tattttacaa
     2941 ttttttagta ttaccattcc tttatgcagt tctgcattct gcaggattgt ttaataaaaa
     3001 aatccgtacc ggaattattg gcagaaaacg agtttacgag gaactgatat taagtgccac
     3061 atctatagat aaaaataaaa aacttatctg gttccactca tcatctcttg gtgagtttga
     3121 acaggcaaaa ccaataatcg aacggcttaa gcaggaaaaa gatgtaaatg ttcttatcac
     3181 atttttttca ccttccggat atgtgaattc aaaaaaatat ccgcacgcgg atctgatttc
     3241 ttacataccg ttcgatacaa aatctaacgc agaaaaattt attgcaataa caaacccaac
     3301 tctttcggtt ataatgcggt atgatgtctg gccgaatatg attacatctt taaataataa
     3361 aaacataccg gtttaccttg ttgatgcaac attaagagat aattcgccac gattattacc
     3421 tttaataaaa tcgtttcata aaattttatt tggaaaattt tccagaatat taactgtctc
     3481 tgattcggat gctgagcaat ttaaatcttt tggatttgat gataaaaaag taagtgcagt
     3541 tggagataca cgattcgaca gagtttatca aagaagtcta ctggcaaaag aaaaacgact
     3601 tataagctct aatgttttaa aggataaaaa gattctagtc gccggcagta catgggagca
     3661 agatgaggaa gtaatcttcc ctgcattttt aaaacttgca aatgtggata agagtgtgct
     3721 aatgattgtt gccccgcacg aacctacttt actccatctt gaaaagatag aaaatgaatt
     3781 tgcaggaaag ttaaagacaa ttcgcttttc gcatcttaat aattactccg atgaacgaat
     3841 aattattgtt gattctattg gtatcctgct tactctatat acttatgcaa acgtggctta
     3901 tgttggcgga agttttaagc aaaacattca taacgtttta gaagcagctg tttatggtac
     3961 tcctgtgctg tttggaccca aaatagaaaa ttcacaagaa gcaacaaaac ttgctgaaat
     4021 tggcggaggt ataatcatta ataacaaacg gcaggcatat cgaaatctta gaaggttatt
     4081 cgaaaatgaa aatttacgta ctactcttgg aaatatttct gc
//
LOCUS       DASWBY010000286         3918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_235827, whole genome shotgun sequence.
ACCESSION   DASWBY010000286 DASWBY010000000
VERSION     DASWBY010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235827"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..762
                     /locus_tag="VF870_05305"
     CDS             136..762
                     /locus_tag="VF870_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX9251635.1"
                     /translation="MKLLVLFTYLIINTAVNQQICIINAQDKHEIIMDCNYTFEEAVA
                     GIEIPESIINQLTLVDVEYYSFDEKLHRGQIVVNRKAAKDIIEIFDFIKTSRFPIDKV
                     IPIVKYNWNDDSSMNDNNTTAFNYRKVQGSKVLSAHSYGLAIDINPMQNPHVKGKVLH
                     PSEGKYDPKIKGTILRDSNLLQEFKERGWQWGGTWRSSNDYQHFEKKN"
     gene            complement(759..1892)
                     /locus_tag="VF870_05310"
     CDS             complement(759..1892)
                     /locus_tag="VF870_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9251636.1"
                     /translation="MLKLFVWTLFFIQLSILAQVKVEIIVISSLPQNQKVFITGNKPE
                     LGNWNPDKISLNKINDSTWSKEFEFTSGETIEFKFTKGSWNGEALNDSGKVTGNNIFK
                     VSTDTTLVYKIKEWGNSRSNITGQITGTIVYHKNFNGRKILPRDIIVWLPPDYDSAPN
                     QYYPVLYVNDGQNIFDPYTSSFGIDWQIDEVADSLIRAKSIQKIIIVGIYNTVKRGSE
                     YNDTKLGKAYLSFIIEELKPFIDRTYRTLPDSKNTAIAGSSGGGLISFILTWEYSDVF
                     SKAACLSPAFKIQNIDYVAPVKNYMGAKKNIKIYIDIGGIGLEEKLQPGVDEMLSVLK
                     EKGFMENEDLFFFKDPLAEHNEAAWSKRVYKFLEFLFPYKADY"
     gene            2009..2536
                     /locus_tag="VF870_05315"
     CDS             2009..2536
                     /locus_tag="VF870_05315"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX9251637.1"
                     /translation="MSLQFEKYQIREFQLNDADAIVNNANNIEVSRFMRDSFPYPYTK
                     ENAVQWINFVKKNYSNLSFAISDESEVIGGIGAVPQTDVHRFSAEVGFWLGQLHWNKG
                     IISKALPVFCNYLFTNFNFNRLFANVFEGNEASKKVLEKTGFILEGTLRKSVFKGNKF
                     VDHHIYGLLKEDFII"
     gene            2533..>3918
                     /locus_tag="VF870_05320"
     CDS             2533..>3918
                     /locus_tag="VF870_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX9251638.1"
                     /translation="MIANHLKLDLIRNIFLKWNGTKADFIQKLPQTASYREYYRISFD
                     DQTVIGVFNEDLKENEAFLSFSRTFNDLNLNVPKVLYTDLDNNVYLLSDLGDITLYSV
                     IHSKQHDYNSSPELITYYKKALEKLQIFQIAADKKSDYKYCYPRAKFDLQSILWDLNY
                     FKYDFLKLGRIGFDEQLLEYDFQKFSEFVASVNTDYFLYRDFQSRNIMVKDDELYFID
                     YQGGRKGALQYDVAALLYDAKAEIPNPLREELLNHYLDKLESDYNLSRTEFMKSYYAF
                     VLIRIMQAMGAYGFRGLFEKKVHLIKSILPARKNLKHLLENGKLDFDIPHLHEVFENI
                     ITSEEFNIYEDRILPNNKLTTTITSFSYKREIPIDLANNGGGYVFDCRGLNNPGRHLE
                     FKLLNGRDAEVIKFLKENSDVDDYLSHIFPIVDSTIETYLERGFKDLMINFGCTGGQH
                     RSVYCADKMYEH"
BASE COUNT         1361 a          594 c          652 g         1311 t
ORIGIN      
        1 gaatccgggt tgtttgaaca accaaaaatg aacactacac taaaaagtgc aataagaata
       61 cttttcatga attttccttt aaattgaggg acgaatatat tcaattattc aaataaaaca
      121 tagacaattt ttattatgaa attactagtg ctatttactt atttaattat taatacagct
      181 gtaaaccagc aaatatgcat aataaatgct caggacaagc atgaaataat tatggattgt
      241 aattacactt ttgaagaagc tgtagcagga attgagatac ctgaatcaat aattaatcaa
      301 cttactttgg ttgatgttga gtattattct tttgatgaaa aacttcacag aggtcaaata
      361 gttgtaaata gaaaagcggc taaagatatt atcgagatat ttgacttcat aaaaacttca
      421 cgttttccca tcgataaagt gattcctata gttaaatata attggaatga tgattcatcg
      481 atgaatgata ataatacaac agcttttaat tatagaaaag ttcagggttc taaggtgcta
      541 tcagcacatt cttacggatt ggcaattgat attaatccaa tgcaaaaccc acatgttaaa
      601 ggtaaagtac ttcatccatc tgagggtaaa tatgatccca aaatcaaagg aactatattg
      661 agagattcaa atttattgca agaatttaaa gaacgtggat ggcaatgggg aggaacctgg
      721 cgaagttcaa atgattatca gcattttgaa aagaagaatt aataatcagc tttatatgga
      781 aataagaact ccaggaactt ataaaccctc ttagaccatg cagcctcatt atgttcagct
      841 aagggatctt taaagaaaaa taaatcttca ttttccataa agcctttttc cttaagaaca
      901 ctaagcatct catctacacc cggttgaagc ttctcctcca aaccaattcc tccaatatca
      961 atataaattt taatattctt ttttgctccc atataatttt taacaggagc aacgtaatcg
     1021 atattttgta ttttaaatgc cggagataaa caggcagctt ttgaaaagac atctgagtat
     1081 tcccaggtta aaataaatga tattaagcct ccgccggaag aaccagcaat agctgtattt
     1141 ttagaatcag gtaatgttct gtaagttcta tctataaaag gttttagttc ttctattata
     1201 aagcttaaat aagccttacc aagttttgtg tcattatatt ctgaccctcg tttaacagta
     1261 ttataaattc ccacaataat aattttttga attgatttgg ctctaatcaa actatctgcc
     1321 acttcatcaa tttgccaatc aattccgaag gaagatgtat atggatcgaa tatattttgc
     1381 ccgtcattca catataaaac aggataatat tgattgggag cagagtcgta gtctggtgga
     1441 agccatacaa ttatatcacg tggaaggatt tttcttccat taaagttttt atgatataca
     1501 attgtaccgg ttatttgtcc agttatattt gatcgggaat tgccccattc cttaatttta
     1561 taaacaagtg tagtatcagt tgaaacttta aagatattat taccagtaac tttaccagaa
     1621 tcatttaggg cttccccatt ccagcttccc ttggtaaact taaactcaat cgtttcacct
     1681 gatgtaaatt caaattcttt agaccaggtt gaatcattaa ttttgttgag tgagatttta
     1741 tccggattcc aattacctaa ttcaggttta ttaccagtaa taaatacttt ttgattctga
     1801 ggtaatgatg agattacaat tatttctact ttaacttgtg caagtatact gagttgtatg
     1861 aaaaataaag tccatacaaa tagctttaac attttcatct aaaattgttt ttattttctt
     1921 caggtaaata tagtaaatta gaagtaagat ttatttctct tagaaatcaa acaagataac
     1981 tattaatata gtaagtgaac tttaatttat gtccctgcaa tttgagaaat atcagataag
     2041 agagtttcaa ttaaacgatg ctgatgcaat agtaaataat gcaaataata ttgaagtatc
     2101 aagatttatg cgggattcat tcccctatcc ttacacaaag gagaatgcag ttcaatggat
     2161 aaatttcgtg aagaagaatt actcaaattt atcttttgca atatcagatg aaagtgaagt
     2221 aataggcggc atcggggcgg taccgcaaac agatgttcac cgcttttctg cagaagtagg
     2281 tttttggtta gggcaacttc actggaataa aggaataata tccaaagcat tacctgtatt
     2341 ttgtaattat ttatttacta attttaattt taaccggctt tttgcaaatg tttttgaagg
     2401 caatgaggca tcaaaaaaag ttttagaaaa gacaggattt atcttagaag gtactctgcg
     2461 caaaagtgta tttaagggaa ataaatttgt tgatcaccac atatacggat tactaaagga
     2521 agattttatt atatgattgc taatcatctt aaacttgatc ttataagaaa tatattttta
     2581 aaatggaatg gtactaaagc tgacttcatt caaaaacttc cgcagacagc ttcatatcgc
     2641 gagtattata gaatttcttt tgatgatcag actgttatag gtgtgtttaa cgaggactta
     2701 aaggagaacg aagcatttct ttctttttca agaacattta atgacttaaa tcttaatgtt
     2761 ccaaaagttt tatacacgga tctggataat aatgtttacc tgctaagtga tcttggtgat
     2821 ataactttgt attcggtcat tcattcaaaa cagcacgatt ataattcttc gcctgaactg
     2881 ataacttatt acaaaaaagc tcttgagaaa ttgcaaattt tccagatagc tgctgataaa
     2941 aagagtgatt ataaatactg ttatccgcga gccaagtttg acctacaatc aattttatgg
     3001 gatttgaatt acttcaagta tgattttctt aagttaggca gaatcgggtt tgatgaacaa
     3061 ttattagaat atgattttca aaagttctcc gagtttgtag catctgtaaa tacggattat
     3121 tttctttatc gtgattttca atcgcgaaac ataatggtaa aagacgatga attatatttt
     3181 atcgattatc agggcggaag aaaaggcgcg ctgcaatatg atgttgctgc acttttgtac
     3241 gatgcaaagg cagaaatacc gaatccatta agagaagaat tattgaatca ttaccttgat
     3301 aaacttgaat cggattataa tttaagccgc acagagttta tgaaatctta ttatgcattt
     3361 gttttgatca gaataatgca ggcaatgggt gcttatggtt ttcgcggact atttgaaaag
     3421 aaagtacatc ttattaaaag cattttaccc gcaagaaaaa atctgaaaca tttattagaa
     3481 aacggtaagc tcgattttga tatcccgcat ttgcatgaag tatttgaaaa tataattact
     3541 tcagaagaat ttaatattta cgaagacaga atattaccaa acaataaact taccacaact
     3601 ataacaagtt tttcatacaa acgggaaatc cctattgatc ttgcaaacaa tggcggcgga
     3661 tatgtttttg attgcagagg acttaataat cccggtagac atttagaatt taaattgctt
     3721 aacgggcgcg acgctgaagt tataaaattc ttaaaagaga attctgatgt tgatgattat
     3781 ttgtcccaca tttttccaat tgtagattcc acaattgaaa cctatcttga acgcggtttt
     3841 aaagatttga tgatcaattt tggatgtacc ggcggtcagc atagatctgt ttactgtgcg
     3901 gacaaaatgt atgagcac
//
LOCUS       DASWBY010000287         3102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_347056, whole genome shotgun sequence.
ACCESSION   DASWBY010000287 DASWBY010000000
VERSION     DASWBY010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347056"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VF870_05325"
     CDS             <1..206
                     /locus_tag="VF870_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX9251639.1"
                     /translation="NIDNANSLKKDIENAGMTSFIQEKNVAGTVFNVVYIGKFETRKE
                     ADDFLPIANTRFRINGRVVEINQ"
     gene            203..985
                     /locus_tag="VF870_05330"
     CDS             203..985
                     /locus_tag="VF870_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX9251640.1"
                     /translation="MKIKFLGTGSGKTSLNRFHSSLLISSSNYNLLVDCGDGISRAIL
                     NQNIDFNSIDSILFSHFHADHFSGLPSMITQMKLLSRKNDLRIFVHSSEKSFLEDFIF
                     HSYLFKKRMTFDLSIVPFDEKDEVLLIDDFRFTSKLNSHLDKYKKASLETPSNQENKL
                     GFASLSFLFKDDGNTCIYTGDVGSEKDLYLFNQKVDWFITEVTHIKSEDSLKIIDLLQ
                     ASKVILTHIGDDFEKSISDFCQTLPENLKSRIIIAFDQLELN"
     gene            1077..2186
                     /locus_tag="VF870_05335"
     CDS             1077..2186
                     /locus_tag="VF870_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9251641.1"
                     /translation="MTREEFKYKNGIIGKTKEINDLVDIIMQVAQSDISILIFGESGV
                     GKEIFAKAIHSHSNRSNKPMVSVNCGAIPEGIIESELFGHKKGSFTGAVESRKGYFEI
                     ADNSTLFLDEIGELPLTTQVKLLRVLENKEFMKLGAETVVRVDVRIIAATNKDLQKEV
                     DARRFREDLFFRLKAVSLNIPPLRKRKPDIEELAKHFIKGYAENNKLTVPEITSDAIE
                     ILKNYTWPGNIRELKNTIETAIALNRGKTLTEEMFSELKSDYVDFDEQRNLPVFIRKS
                     PEDADRELMYRALFEIKKDVMELKDLIVYRLNIDDYNQREKDDDVLSIRELEKDAIVK
                     ALDKTKGSKRKAAKLLNISERTLYRKLKEYDIIDS"
     gene            2209..2709
                     /gene="lptE"
                     /locus_tag="VF870_05340"
     CDS             2209..2709
                     /gene="lptE"
                     /locus_tag="VF870_05340"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX9251642.1"
                     /translation="MFLNLLFIGIIAINFTSCCAYSFTGASVPEHLKTIAIPIADDRS
                     GSGEPGLREDLTQTLIEKFINDNTLQVSERTSANALLECSIVSLNDAPAIVSAGEDIT
                     SRRITIRVKVVYRDLVKRTTIFEKTFSNYSDYPASDPINGRQTAISTAIDLISEDILL
                     DTVSGW"
     gene            2732..>3102
                     /locus_tag="VF870_05345"
     CDS             2732..>3102
                     /locus_tag="VF870_05345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9251643.1"
                     /translation="MEQVVLIGYFVSLSILFIFGLHGFVMMYYHRKYGHKIPVANKDF
                     KDDVFVTIQLPLYNEMYVIERLIDSLCAIDYPKDKMEIQVLDDSTDETTSIVARAVEE
                     KKKLGYDISHIRRGSREGFKAG"
BASE COUNT         1128 a          446 c          551 g          977 t
ORIGIN      
        1 caaatattga taatgcaaat tcgttaaaaa aagatattga aaacgccgga atgacatcat
       61 tcattcagga aaaaaatgtt gcgggaacgg tttttaatgt agtttatatt ggaaagtttg
      121 aaacacgaaa agaagcagat gattttcttc caattgcaaa tacaagattt agaattaacg
      181 gcagagttgt tgagataaat cagtgaagat aaaattttta ggaacaggtt ccggtaaaac
      241 ttctctcaat cgtttccatt catcattact tatttcttct tcaaattata atttattggt
      301 tgattgcggt gatggcattt caagagctat tttaaatcaa aatattgatt tcaattcgat
      361 agattcaatt ttattttcac attttcatgc agatcatttt tccggcttgc cctctatgat
      421 aacacaaatg aaattattat caagaaaaaa tgatctgaga atttttgttc attcatctga
      481 aaaatctttt ttagaagatt ttatttttca ttcctatcta tttaaaaaac gaatgacttt
      541 tgatctgagc atagttcctt tcgatgaaaa agatgaagtt ttattgattg atgattttag
      601 attcacctca aagttgaatt cacatctgga caaatacaaa aaagcttctt tagaaacacc
      661 gtctaatcag gaaaataaat taggttttgc ttcgttaagt tttcttttta aagatgatgg
      721 aaacacttgc atttacactg gagatgtcgg tagtgaaaaa gacttatatc ttttcaatca
      781 aaaagttgat tggtttataa cggaagtaac tcatatcaag agtgaagatt cactcaagat
      841 aattgatcta cttcaagcat caaaagtaat cttgacccac attggtgatg attttgaaaa
      901 atcaataagt gatttttgtc aaacactgcc agaaaatcta aaatcccgca ttattatagc
      961 atttgatcaa cttgaattaa actagtccat ctaaatgcgt ctttaataaa atcctacatt
     1021 atgctatatt tggcatacaa attttacaat atggcagaca aatcttaaac tattgaatga
     1081 caagagaaga attcaaatat aaaaacggaa ttatcggtaa aaccaaagag ataaacgatc
     1141 tggttgatat aattatgcag gttgcccaat ccgatatttc aatcctaatt tttggtgaaa
     1201 gcggagttgg taaagagatt tttgcaaaag caattcactc acacagtaat cgctcgaaca
     1261 agccgatggt ttctgtaaac tgcggtgcta ttccggaagg aataattgaa agcgaattgt
     1321 tcggacataa aaaaggatca tttaccggcg cagttgaatc ccgcaaaggc tattttgaaa
     1381 tcgctgacaa tagcacatta tttttagatg aaattggcga acttccactg actacacaag
     1441 taaaattatt acgcgtactt gaaaataaag aatttatgaa actcggtgcc gaaaccgttg
     1501 taagagttga tgtgagaatt attgccgcca ctaataaaga tttacaaaaa gaagtcgatg
     1561 caagaagatt tagagaagat ttatttttca gattaaaagc tgtttcgcta aacataccgc
     1621 cgttaagaaa acgaaaacct gatattgaag aacttgcaaa acattttatt aagggctatg
     1681 ctgaaaacaa taaacttacc gttcctgaga taacatctga tgctattgag atcttaaaaa
     1741 attatacttg gccgggcaat attcgcgaat taaaaaatac aattgaaaca gctatcgcat
     1801 taaacagagg aaaaacttta acagaagaaa tgttttcaga attaaaatca gattatgtcg
     1861 attttgatga acaaagaaat ttacctgtgt ttattagaaa atctcccgaa gatgctgaca
     1921 gggaattgat gtaccgtgct ctttttgaaa tcaaaaaaga tgtaatggaa ttaaaagatt
     1981 tgattgttta tcgccttaat attgatgatt ataatcaaag agaaaaagat gatgatgtat
     2041 tatcaataag agaacttgaa aaagatgcca ttgtaaaagc tctagataaa acaaaaggca
     2101 gtaaacgaaa agcagccaaa ttgctaaaca taagcgaaag aacattatac agaaaattaa
     2161 aagagtatga tatcattgat tcataaaaaa agcaggctaa tattagttgt gtttttaaac
     2221 ctattgttta taggaataat tgcaattaat tttacttcgt gctgtgctta ttcttttacc
     2281 ggtgcatctg ttccggaaca tttgaaaaca atagcaatcc cgattgcgga tgatagaagc
     2341 ggttccggtg aacccggttt aagagaggat ttaactcaaa cattaataga aaaatttatt
     2401 aatgataaca ctttgcaggt ttcagaacgt acatctgcaa atgctctttt agaatgttca
     2461 attgtttcac ttaatgatgc tccggcaatc gtatctgcag gtgaagatat tacatcgaga
     2521 aggattacaa taagggttaa agttgtttat cgtgatttag tgaaacgcac aacgattttt
     2581 gaaaaaacat tttcaaatta tagtgattat cctgcaagtg atccgataaa cggaaggcag
     2641 acagcgataa gcactgcaat agatcttatt agtgaagaca ttttgttaga taccgtatcg
     2701 ggttggtaat aagagaggat ttttaattta aatggaacaa gtagttttaa taggttattt
     2761 tgtctcgctt tcgatccttt tcatatttgg actgcatggt tttgtgatga tgtactacca
     2821 tagaaaatat ggtcacaaaa ttcctgtagc caataaagat tttaaagatg atgtatttgt
     2881 tactatccag cttccactct ataatgaaat gtatgtaatt gaaagattaa ttgattcttt
     2941 atgcgcgatt gattatccaa aagataaaat ggaaatacag gtgcttgatg attccactga
     3001 tgaaacgact tcgatagttg ccagagcagt tgaagaaaaa aagaaattgg gatatgatat
     3061 ttcacacata agacgaggct cacgggaagg ttttaaggca gg
//
LOCUS       DASWBY010000288         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_85131, whole genome shotgun sequence.
ACCESSION   DASWBY010000288 DASWBY010000000
VERSION     DASWBY010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85131"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VF870_05350"
     CDS             <1..218
                     /locus_tag="VF870_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9251644.1"
                     /translation="LTPDSSRFWPLDKYEKGKSQESFDKQFVRDYLISIKFNKQPPPP
                     PLPEDIIQRTSEKYLEALEKLTGKSIS"
     gene            219..527
                     /locus_tag="VF870_05355"
     CDS             219..527
                     /locus_tag="VF870_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX9251645.1"
                     /translation="MSPVKIKLLEKKKLLIVWDDGDESIIELRELRKRCPCATCLAER
                     DKQSKMYIPIFAENQVTVKSIDQVGNYAVQITWNDGHSSGIYEYHFLKRFIEKTTIKK
                     "
     gene            524..1129
                     /gene="pgsA"
                     /locus_tag="VF870_05360"
     CDS             524..1129
                     /gene="pgsA"
                     /locus_tag="VF870_05360"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX9251646.1"
                     /translation="MTLPNQLTILRIILTPVFLYLFLSNDPLLIQISLGVFLIAALTD
                     WYDGWLARKFNYITDWGKFWDPLADKILTSAAFIGFVLVGLVELWMVVLIIFRDLIVT
                     LLRIYAESRGYNFVTSYYAKWKTVFQMVFLYYLLLLYGGLNTIEVYSGNEHLFSLLSN
                     KNLIYGIMLVITVITVHSGVTYLLKNKHLIKKLFNETNKLV"
     gene            1107..1568
                     /locus_tag="VF870_05365"
     CDS             1107..1568
                     /locus_tag="VF870_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX9251647.1"
                     /translation="MKQINWFEKLVGSGFYTGYIPIASGTFGSLAAILIYLIPDFERL
                     EIIIPAIILLFLYGLIVSSKFEKVYGKDPSQCTVDEVVGTWIALIALPKTIFIVLTSF
                     LVWRALDIIKPFPAGISEKLPGGLGIMTDDVISGFYSLIILHLIVYTFGVY"
     gene            1571..>2010
                     /locus_tag="VF870_05370"
     CDS             1571..>2010
                     /locus_tag="VF870_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX9251648.1"
                     /translation="MKAYLISIGDELLIGQTINTNVSFIGNLISDINLLIVKTAAIAD
                     DLTAILEELDLAASKADIIICTGGLGPTHDDVKRNAFVQYFKTELVHNNEVLEDIKSM
                     LKRRGRELKTTHEDQAMVPKIAQVIRNENGTAPGYWIEKDKKIF"
BASE COUNT          666 a          296 c          373 g          675 t
ORIGIN      
        1 tattaacacc tgattcttca agattctggc ctttggataa atatgaaaaa ggaaaatcac
       61 aggaaagctt tgataaacaa tttgttcgtg attatttaat ctcaatcaag tttaacaagc
      121 agcctccacc gcctcctttg cctgaagata ttatccagag aacaagcgaa aaatatttag
      181 aagctttgga aaaacttacc ggcaagagta tttcttaaat gagcccggtt aagataaaac
      241 tacttgaaaa gaaaaaactt ttaatcgtct gggatgatgg agatgaaagt ataattgaat
      301 taagagagtt aagaaaaaga tgtccctgtg caacctgttt ggctgaacgg gataagcaaa
      361 gtaaaatgta catccccata tttgcagaaa atcaggtaac cgttaaatca atagatcagg
      421 ttggtaatta cgcagtgcag ataacgtgga atgacgggca tagcagcggt atttatgagt
      481 atcattttct taaaagattt atagagaaaa caacgattaa aaaatgacac tcccgaatca
      541 attaacaatt cttagaataa tactaactcc ggtgttcttg tatcttttct tatcaaacga
      601 tccattgcta attcagatat cattaggagt atttttaatc gcggcgttaa ctgattggta
      661 tgacggctgg cttgcaagaa aatttaatta tataactgat tggggaaaat tctgggatcc
      721 tcttgcagat aaaattttaa cttcagcggc ttttattggt tttgttttag ttggattagt
      781 cgaactatgg atggttgttc ttataatttt tcgtgatttg attgttacac tattaagaat
      841 ctacgctgaa agcagaggat ataattttgt tacaagttat tatgcaaagt ggaagactgt
      901 ttttcaaatg gtgtttttgt attatctact cttactctac ggcggactaa atacaattga
      961 ggtttattct ggtaacgaac atttgttcag ccttttatca aacaaaaatt tgatttatgg
     1021 tataatgctt gtaattacag tgattactgt tcattccggt gtaacatatc ttttgaaaaa
     1081 taaacattta ataaaaaagc tttttaatga aacaaataaa ctggtttgaa aaattagttg
     1141 gctctggatt ttatactggt tacattccta ttgcatcggg aacatttgga agtttagcag
     1201 caattttaat ttatcttatt cctgattttg aaagacttga aattataatt ccggctataa
     1261 tacttttatt tctttatggc ttaattgttt catctaaatt tgaaaaagtt tatggcaaag
     1321 atccatctca atgtactgtt gatgaagttg ttgggacatg gattgcattg atagctttac
     1381 caaaaactat atttattgtg cttacatcat tcttagtctg gcgtgcactg gatattatta
     1441 aaccctttcc ggcaggaatt tctgaaaaat taccgggcgg cttggggata atgactgatg
     1501 atgtcatctc aggtttttat tccttaatta ttcttcacct aattgtttac acattcggag
     1561 tttattaaaa atgaaagctt atttaatttc cattggggat gaattattaa tcgggcaaac
     1621 gattaataca aatgtttctt ttattggaaa tctgatctct gatattaatc tattgatagt
     1681 taaaactgct gctatagcag acgatttaac agcaatctta gaggaacttg atttagctgc
     1741 atcaaaggca gatattatta tttgtaccgg cggattgggg cccactcacg atgacgtgaa
     1801 aagaaatgct ttcgttcagt attttaaaac tgaattagtg cataacaatg aagtgctgga
     1861 agatataaaa agcatgctta aacgtcgtgg aagagaatta aaaacaacgc acgaagatca
     1921 agctatggtt cccaaaattg cgcaggttat ccgtaatgaa aatggaaccg cacccggtta
     1981 ctggattgaa aaggataaaa aaatatttat
//
LOCUS       DASWBY010000289         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_497919, whole genome shotgun sequence.
ACCESSION   DASWBY010000289 DASWBY010000000
VERSION     DASWBY010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497919"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="VF870_05375"
     CDS             <1..101
                     /locus_tag="VF870_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor"
                     /protein_id="HEX9251649.1"
                     /translation="LKKYGIIKGGWLGIKRISRCHPWGGSGYDPVP"
     gene            112..846
                     /gene="mtgA"
                     /locus_tag="VF870_05380"
     CDS             112..846
                     /gene="mtgA"
                     /locus_tag="VF870_05380"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX9251650.1"
                     /translation="MAKKPSSKKISFRKRIFNTFKWILKFAVIFFVTTVLLIFAMRWI
                     NPVTSSIMVQRQISGLFSGEFELVNYHWVNYDDVSKFMPIAIVAAEDQNFPNHFGFDF
                     KQIEKALKENKRGRRVRGASTITQQVAKNLFLWEGKSFVRKGIEAYFTLLIELLWDKK
                     RILEVHMNIAEMGDKIFGVGTACVAYFKKPASKITISQAALLAAILPNPRKYSAVRPT
                     GYIRGRQNWIIRQINSLGGPDYLKDL"
     gene            1038..1730
                     /locus_tag="VF870_05385"
     CDS             1038..1730
                     /locus_tag="VF870_05385"
                     /inference="COORDINATES: protein motif:HMM:NF021554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HEX9251651.1"
                     /translation="MSEEIPYPLNYYSLNHLKKSRLDIATQSTFVNSERLMNDGTSWT
                     EYSDINYLRLSSMIGLMAATNTVAYIYQRKVWYTEETTVFHSLEFINDWNKYQQMDKF
                     GHFSDAYFTSDLTGKIYRWSGMSGNTSVWLGAITGWAWMLEIELSDAFMAEWGFSWGD
                     MLANTAGSAFYILQQFNYEALGGIHPKFSWHKSEAWKENRYNKDPQALIEDYEGMTFW
                     LTVNPHHYFPDS"
BASE COUNT          623 a          280 c          370 g          607 t
ORIGIN      
        1 cgttaaaaaa atatggaata attaaaggcg gttggttagg aataaaaaga atttcacgct
       61 gtcatccctg gggaggcagt ggttatgatc ctgtaccata aataaaattc tatggcaaaa
      121 aaaccatcat caaaaaaaat atctttcaga aaaagaatct ttaacacctt taaatggatt
      181 ttaaagtttg cagtaatttt ctttgttaca actgtgcttc ttatttttgc aatgagatgg
      241 attaatccgg ttactagctc aattatggtt caaagacaga tttccggatt gttctctgga
      301 gaatttgagt tagtaaatta tcactgggtt aattacgatg atgtttctaa gtttatgccg
      361 attgcaattg tagctgctga agatcaaaac tttccaaatc attttggatt tgattttaaa
      421 cagatagaaa aagctttaaa agaaaataaa cgcggaagaa gagttcgcgg cgcaagcacg
      481 attacacagc aggtagcaaa aaatcttttt ttgtgggaag gaaaaagttt tgtgcgcaaa
      541 ggaattgaag catactttac attattaata gaacttttgt gggataaaaa acgaattcta
      601 gaagtacata tgaatattgc tgagatgggt gataaaattt ttggagttgg gactgcttgt
      661 gttgcttatt ttaaaaaacc tgcttcaaaa attacaattt ctcaagctgc attgttagca
      721 gcaatattac caaatcccag aaaatattct gcagttagac ccactggtta tattagggga
      781 agacaaaatt ggattatcag gcaaataaat tcacttggtg gtccggatta tttaaaagat
      841 ctgtaagaaa agagtatctg gtaaattgat tcctgtttta atcaaaatat tttttgaaaa
      901 tgattattga gaatatgaat ctggcaacaa aaatattatt ttttttatta ctctttatat
      961 tttctaaagc ttttgcgcaa tattcaactt cccctactat agactacata aattactcag
     1021 ctcagttgag atctcatttg tctgaagaaa ttccctatcc gctaaactat tattcattaa
     1081 atcatcttaa aaaatctaga ctagatattg caactcaatc aacttttgta aacagtgaaa
     1141 gacttatgaa tgatggaaca agttggacag aatattcaga tattaattat cttcgcctaa
     1201 gttctatgat tggtttaatg gcggctacca acacggtggc atatatttat cagcgcaagg
     1261 tatggtatac tgaagaaaca actgtttttc attctttaga gtttatcaac gattggaaca
     1321 aatatcagca gatggacaaa ttcggtcatt tttcagacgc atattttacg agtgatctta
     1381 cgggaaaaat atatcgatgg tctggaatgt ctggaaatac ttctgtgtgg ctgggagcca
     1441 taacaggctg ggcctggatg cttgaaattg aattatctga tgcctttatg gcagaatggg
     1501 gatttagctg gggagatatg cttgccaata ccgcaggttc tgccttctat attctgcagc
     1561 aatttaacta tgaagcgtta ggtggaattc atccaaagtt tagctggcat aaatcagaag
     1621 cctggaagga gaatcgatat aataaagatc cgcaagcttt aatagaagat tacgaaggta
     1681 tgactttctg gttaacggtt aatccccacc actattttcc cgatagctag aaaaaaaatt
     1741 attctgaatg gttagcacca ttaggcctgg cagttggtgt tagtgtaaaa aatattggta
     1801 tttatccctg gggcggatat aaagaatact ttgttgggct tgatattgat ctgagaaagc
     1861 ttcccatttg ggatgattgg
//
LOCUS       DASWBY010000290         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_236403, whole genome shotgun sequence.
ACCESSION   DASWBY010000290 DASWBY010000000
VERSION     DASWBY010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236403"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VF870_05390"
     CDS             complement(<1..1294)
                     /locus_tag="VF870_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEX9251652.1"
                     /translation="MAQNGEREQWGTKIGLILAVAGNAIGLGNFLRFPVQAAQNGGGA
                     FMIPYFIFFILLGIPLMWIEWGIGRHGGKFRHGSAPGMFDVLWKNKLAKYFGSLGLFI
                     SITILIYYTYIESWTLGFSLFSILGLYSNETRETMTGFLSSYQGITTSQTFSSIWPAY
                     GLMLLTFAINFAVLYKGISKGIEKLAKIAMPLLFIFAIILAIRIFTLGTPDPAFPENS
                     VANGFAFIWNPDFSALGNPNIWLAAAGQIFFTLSVGMGTIHAYASYLKPDDDIVLSGL
                     ATASTNEFAEVILGASIAIPVAVAFFGLDATKEIAQGGAFNLGFVSMPIIFGSAHFPM
                     GELFGFMWFLLLFFAGITSSVAMGQPVVAFLEDEFGMDRKKAVTTLAIAVLIAVQFVI
                     FFLKYGFLNEMDYWAGTFGLVVFALIETVLFMWVFGADK"
     gene            1495..2130
                     /locus_tag="VF870_05395"
     CDS             1495..2130
                     /locus_tag="VF870_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX9251653.1"
                     /translation="MAKKFVSNKDETVRMFKSDFMESLSRVHPSVPLIIFVPVILYML
                     YLSIFDFKIGILSILGLVLFGLFIWTITEYLLHRFIFHLELKSKIGARIHFIFHGVHH
                     DYPSDSRRLVMPPSVSIPLAALFYFLFRLLIGDISVHPFFAGFLIGYLFYDITHYAVH
                     HFNMHSKFWLAIKNHHIKHHYQDPDRGYGVSSPLWDYVFRTNYVRKDEQKA"
     gene            2185..>3117
                     /locus_tag="VF870_05400"
     CDS             2185..>3117
                     /locus_tag="VF870_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX9251654.1"
                     /translation="MKLFYFVFIFISQIIFVNCHTSISDNIYIRSNQIGYLPGDLKTA
                     IILSKTDLKNKPFFILENNSSKIKFEGSVTDSPSDYGSFNFCYEIDFSDLNTPGTYKI
                     KLSGTESYPFQIGDSIFNNVRDSLSLFFKAQRCGPTKPILHQPCHLSDAAKIIGYKNS
                     TARDLTGGWHDAGDYVKFLKTTAYTTYMLLFSYEFDPQKFGYDLDKNDVPDILEEAKV
                     GLDWLLRCNVDNKTLVSQVQNENDHNIGWRLPENDSLQFERPAFVSIGKNTIGIYSAV
                     LALASKIWKEKFYDDEFSTKCLNTAEKFYSLRNNV"
BASE COUNT         1045 a          550 c          517 g         1005 t
ORIGIN      
        1 ctttatcagc gccaaatacc cacataaata agacagtttc aatcaaagca aaaacaacaa
       61 gaccaaaagt accggcccaa taatccatct catttaagaa tccatacttt aaaaagaata
      121 tcacgaattg aacagcaatc agtacagcaa tagctaaggt agtaacagct ttttttctat
      181 ccatcccaaa ctcatcttca aggaatgcaa ctaccggttg tcccattgca acagatgagg
      241 ttattccggc aaagaagagc agcaagaacc acataaatcc aaatagctca cccataggaa
      301 aatgtgcact gccaaatata attggcattg aaacaaaacc aagattaaaa gcaccgcctt
      361 gtgcaatctc ttttgttgca tcaagcccaa agaatgctac tgctacagga attgcaattg
      421 aagcaccaag aattacttct gcaaattcat ttgttgatgc agttgctaat cctgataata
      481 caatatcgtc atctggtttt aaataacttg cataggcatg tatcgttccc attccaaccg
      541 aaagagtgaa gaaaatttgg cccgcggcgg caagccagat attgggattt cctaaagccg
      601 aaaaatcagg gttccaaata aatgcaaatc catttgcaac tgaattttcc ggaaaagccg
      661 gatccggtgt tcccaaagtg aatattctaa tcgccagaat aattgcaaaa atgaaaagaa
      721 gaggcatagc aatttttgca agcttttcta ttccttttga aattcctttg tatagtactg
      781 cgaagtttat tgcaaaagtt aatagcatca gtccataagc tggccatatt gagctgaatg
      841 tttgtgaggt tgtaattccc tgataactgg aaaggaagcc ggtcattgtt tctcttgttt
      901 cgttggaata taaaccaagt atagaaaaaa gactaaatcc aagtgtccag gattcaatgt
      961 acgtgtaata aataagaata gttattgata taaataaacc aagtgagcca aagtattttg
     1021 ccagtttatt tttccagagc acatcaaaca taccaggtgc ggagccatgt ctaaatttac
     1081 caccgtgcct tccgattccc cattcaatcc acattaatgg aatgccaaga agtatgaaga
     1141 aaataaaata gggaatcata aaagcaccac cgccattttg tgctgcttga accggaaatc
     1201 taagaaagtt tcctaatcca atggcgttgc cggctacggc taaaatcaaa cctatttttg
     1261 ttccccattg ctctcgttcg ccattttgag ccatcaaacc tcctggaaat taaggaattt
     1321 ctgaaattta actgtgaata tgaatacttt actcattaat atcaaatata attgacagaa
     1381 taaacaagtg ttttttatca actttcagag aatcactaca aaaattttta taatcatttt
     1441 tattcttcaa ttactaatta gtaaatttgc cgcgttaaat aaataggata tgaaatggca
     1501 aaaaagtttg tttccaataa agacgaaact gtaagaatgt ttaaaagtga ttttatggaa
     1561 tcactatcac gagttcatcc ttcagttcca ctgataattt ttgttccagt tatactttat
     1621 atgttatact tgtcaatttt tgattttaaa attggaatat tatccatatt gggattagta
     1681 ctattcggat tatttatctg gaccattaca gaatatttac ttcatagatt tatctttcat
     1741 cttgaactga aaagtaaaat aggtgcaagg attcacttca tttttcatgg agtacatcac
     1801 gattatccaa gtgattcaag acgattggta atgccgccat ctgtaagtat tccacttgct
     1861 gcattatttt acttcttgtt tagactttta attggtgata tttctgttca tccattcttt
     1921 gcgggatttt taatcgggta tttattctac gatattaccc attatgctgt tcatcatttt
     1981 aatatgcaca gtaaattttg gttagctatt aagaatcatc atattaaaca tcattaccag
     2041 gatcctgata gaggttacgg agttagttct ccattatggg attatgtttt tagaacaaat
     2101 tatgttcgta aagatgagca aaaagcttaa ttagattttt agttgggcaa ttagcaggag
     2161 attataatta ttaaaaatgg atttgtgaaa cttttctatt ttgttttcat ttttatctca
     2221 caaattatct ttgttaattg ccatacctct atttccgata acatttatat ccgatccaat
     2281 caaattggtt atttacccgg tgatttaaag acagcaatta ttctttccaa aactgatcta
     2341 aagaacaaac cgttttttat attagaaaac aattcaagta aaataaaatt tgaaggcagc
     2401 gtaacagatt caccctcaga ctatggcagt tttaactttt gctatgagat tgatttttct
     2461 gacttaaaca ctccgggtac ttataaaata aaattatcag gtaccgagtc atatccattc
     2521 caaattggtg attcaatttt taataatgtg cgtgattcac ttagcctttt ctttaaagca
     2581 cagcgttgtg gtcctaccaa acctattcta catcaacctt gtcatctttc agatgctgca
     2641 aaaattattg gttataaaaa ttccacagca cgcgatttaa ccgggggctg gcacgatgcg
     2701 ggggattatg taaagttttt aaaaacaacg gcatacacaa catatatgct gttattcagt
     2761 tatgagtttg atccgcaaaa atttggatac gatcttgata aaaatgatgt acccgatatt
     2821 ctggaagaag caaaagttgg tcttgattgg ctgttgagat gcaatgttga taataaaact
     2881 ttggtttccc aggtacaaaa tgaaaatgat cataacattg gctggagatt gccggaaaat
     2941 gacagcctgc aatttgaacg cccggctttt gtaagcatcg gaaaaaatac aattggaatt
     3001 tattctgccg tgttagcttt agcatcaaaa atatggaaag agaagtttta tgatgatgaa
     3061 ttttcaacca aatgtttgaa tactgctgaa aaattttatt ctcttagaaa taatgtt
//
LOCUS       DASWBY010000291         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_236512, whole genome shotgun sequence.
ACCESSION   DASWBY010000291 DASWBY010000000
VERSION     DASWBY010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236512"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VF870_05405"
     CDS             complement(<1..950)
                     /locus_tag="VF870_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX9251655.1"
                     /translation="MSDKNRRVVVTGIGALTPIGLTVNEFWNSMMESKSGAAMIKQFD
                     TSKIETKFACELKGFDPLNYLDKKIARRLDPFAQYALVSSRQAVEDSNLQPEKLSEEE
                     KNRIGVIFGSGIGGIQTFYDQAVINREQGPGRISPFFIPMLIPDIAAGHISMQYGFRG
                     PNYCTVSACATGNNNMIDSFLLIKFGMADVMVAGGSEASISELGMGGFNASRALSTRN
                     DSPETASRPFDATRDGFVMGEGGGTVILEELEHALKRNAKIYCEIVGAGLSADAHHIT
                     APHPEGTGAILSMKMAIQQANVKPEEIDYVNMHGTSTPLG"
     gene            complement(1082..1549)
                     /locus_tag="VF870_05410"
     CDS             complement(1082..1549)
                     /locus_tag="VF870_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9251656.1"
                     /translation="MDNKIFIRNASENDVPTIFALIRELAEFEKLADQIKTTEDELRK
                     TLFGKDRFVEILLAEYEGIIVGQALFFKNFSTFLGKPGIYLEDLYVKPEMRGKGIGKA
                     LLFKIISIAKERNYGRVEWSVLDWNEPAIDFYQKIGAIPLNDWTMFRLTADKF"
     gene            complement(1554..2303)
                     /locus_tag="VF870_05415"
     CDS             complement(1554..2303)
                     /locus_tag="VF870_05415"
                     /inference="COORDINATES: protein motif:HMM:NF025115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HEX9251657.1"
                     /translation="MKKFFILFYLMSFSCLLSANNYIFTDKEFNKADTLEIKTLNYSN
                     STKDSTVIINIDYPQIEGLENAAIERKVNLFLEEEFKQSIAWFDEVKSDTAFFEDFSS
                     EMQYTFETGFQTEFNSKNFISIVLSHYQFTGGAHGNYFALGYNINLKDGKVLSLKDII
                     REDSFDLLTYECEQAILEKFEANSLIDAGLFEDEIEILEDQDFYIMPGMLVLQFDPYE
                     IGPWSMGEITAEIPFEKIKDILKENLPFPTN"
     gene            complement(2326..2487)
                     /locus_tag="VF870_05420"
     CDS             complement(2326..2487)
                     /locus_tag="VF870_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9251658.1"
                     /translation="MLISISRVYLGLHYPSDIFGGAIIGLAFGYLFASGVLYLEKYLA
                     DRKIKHSES"
BASE COUNT          799 a          480 c          383 g          838 t
ORIGIN      
        1 tcaccaagtg gagtagaagt tccgtgcata tttacatagt caatttcttc aggtttaaca
       61 ttggcttgct gaatagccat tttcattgat aaaatagcac cagtaccttc cgggtgtgga
      121 gcagttatgt gatgagcatc agcagataat cctgcaccga ctatttcgca gtaaattttt
      181 gcattacgtt ttaatgcatg ttcaagttct tcaagaatta cagttccgcc tccttcaccc
      241 ataacaaatc catctcgcgt tgcatcaaaa ggacggcttg cagtttcagg tgaatcattt
      301 cttgttgata aggctctgct ggcattaaat ccacccatac ctagttctga gatagaggcc
      361 tcacttccac cagcaaccat aacatcagcc attccaaatt ttataagcag aaagctgtca
      421 atcatattat tatttccagt agcacaagct gaaacggtac agtaatttgg cccacggaaa
      481 ccatattgca tagaaatgtg tccggctgca atatcaggaa taagcattgg aataaagaat
      541 ggtgaaattc tgccgggccc ttgttcacga tttattactg cctgatcata aaaagtctga
      601 ataccgccga tgccgcttcc gaagattaca ccgattcggt ttttctcttc ttcagaaagt
      661 ttttcaggct gcagattact gtcttcaact gcctgtcgtg atgaaaccaa agcatactgt
      721 gcaaatggat ctaatcttct tgcaattttt ttatcaaggt aatttaaagg gtcaaatcct
      781 tttaattcgc acgcaaattt tgtttcaatt tttgatgtat caaattgctt tatcattgct
      841 gcgccgcttt tggattccat catactattc cagaattcat taacagttaa tccaattgga
      901 gttaaagctc cgattcctgt tacaactact cttctgtttt tgtcagacat ttagatcctc
      961 ttttaagatg aaaaattttt agttcttatg aatattttga agcataaaaa taatgaattt
     1021 taagtaaaag cttaagcgaa atttgacaca ctaaattgat tgaaatggca acgattaaaa
     1081 actagaactt atcggcggtc agtctgaaca tagtccaatc attcaatgga attgcaccaa
     1141 ttttttgata aaaatcaatt gcgggctcat tccaatctaa aaccgaccat tcaactcttc
     1201 catagtttct ttctttagca attgatatga ttttgaagag caaagcttta ccaatccctt
     1261 taccccgcat ttccggtttt acgtaaagat cttcaagata tattccgggt ttaccgagaa
     1321 atgttgaaaa gtttttaaag aataatgctt gtccaacaat tataccttcg tactcagcaa
     1381 gaagaatttc tacaaaccta tctttgccaa atagtgtttt acgtaattca tcttctgtag
     1441 tttttatttg atcagcaagt ttttcaaatt ctgctaattc tcttatcaac gcaaaaatgg
     1501 ttggaacatc attctctgag gcattgcgaa taaatatttt gttatccata atattaattt
     1561 gttgggaatg gaagattttc tttcaagata tcttttatct tttcaaatgg aatttctgct
     1621 gtaatctcac ccattgacca gggaccaatt tcatatggat caaactgaag aacaagcatt
     1681 cccggcataa tgtaaaaatc ctgatcttca agaatttcaa tttcatcttc aaatagtcct
     1741 gcatcgatca atgaatttgc ctcaaacttt tctaagatgg cctgctcgca ttcataagtt
     1801 aatagatcaa acgagtcttc tctaataata tcttttaatg ataagacttt cccatctttc
     1861 aaattgatgt tatatccaag tgcaaaataa ttgccatgtg caccgccggt aaactgataa
     1921 tggcttaaca cgatgcttat aaaatttttg gaattaaact cagtctgaaa tccggtttca
     1981 aacgtgtatt gcatttcact ggaaaaatct tcaaaaaaag cagtatcaga tttaacctca
     2041 tcgaaccacg caattgattg tttgaattct tcttctaaaa acaaattcac tttccgttca
     2101 attgctgcat tttccaaacc ttcaatttga ggataatcta tatttataat tactgtactg
     2161 tccttagtac tattcgagta attcaaagtt ttgatttcaa gagtatcggc tttattaaat
     2221 tctttatcag taaatatgta attattagct gataacaggc agctaaaact cataaggtaa
     2281 aagagaataa aaaatttttt catttatgta taacctttta aaaaattatg attcagaatg
     2341 tttgattttt ctatcagcca aatacttttc taaatacaaa actccggaag cgaataaata
     2401 tccaaaagct aatccaatta tcgcacctcc aaaaatatcg gaagggtaat gtaaacctaa
     2461 ataaactctg gatattgata tcagcaaagc ggtgataaac
//
LOCUS       DASWBY010000292        10381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_610692, whole genome shotgun sequence.
ACCESSION   DASWBY010000292 DASWBY010000000
VERSION     DASWBY010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10381
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610692"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF870_05425"
     CDS             complement(<1..180)
                     /locus_tag="VF870_05425"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX9251659.1"
                     /translation="MQSIIRIKKATFYGYHGVMSEEQSVGGKFEADVDIYTDFSEAAK
                     KDSLNQTIDYHKVYKV"
     gene            complement(223..1140)
                     /locus_tag="VF870_05430"
     CDS             complement(223..1140)
                     /locus_tag="VF870_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9251660.1"
                     /translation="MRSTPLRIFLVYALLCFIWGSTWLAIRASLESLTPFISVGSRFL
                     LASLFVFALMKIRGVSLQTDKESIRLYFLMGFLSFVIPFGLVYWAEQFVPSGLASVLF
                     GVYPFFVALFSFFRIPDESIGKIKILGMTLGFIGIVVIFSDSFTTDLSTLFIGMVAVA
                     LSGIIQAWIAVSIKKSGKHLNPLSMNLVPMFIAGISGLVFGLLLEDISKIKIDNTAII
                     SVLYLAFFGSVVTFTSFYWLMKKINVVLLSLVAFITPIVALILGWIFYNETLTTQHII
                     GSLLVLIGLLFANLEAVFNRKQRFENQLD"
     gene            complement(1142..1876)
                     /gene="pgeF"
                     /locus_tag="VF870_05435"
     CDS             complement(1142..1876)
                     /gene="pgeF"
                     /locus_tag="VF870_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX9251661.1"
                     /translation="MYILKPYIFNNHPEIIFGFSTKSAGNAKPPFYFNLSYSVGDDKS
                     IVDKNRKEFFEAIGLSVDTVGYQRQIHSDIVQVIGCSGDNGASDALITSEKNLGLAIA
                     VADCNPIFIYDKKNGVIAAVHSGWQGTEQKILLKTLLKLQKDYLSKPENMIVYIGPSI
                     SQTNYEVGKEVAEKFDQTFVKPKGEKFILDISGINYKMLLDFKIPKNQIQKSELCTFE
                     FNSLLHSYRRDGNLSGRSLGVIAIKG"
     gene            1998..2777
                     /gene="surE"
                     /locus_tag="VF870_05440"
     CDS             1998..2777
                     /gene="surE"
                     /locus_tag="VF870_05440"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX9251662.1"
                     /translation="MRILVSNDDGIDSVGIFSLVEALQEIAEVTVVAPHKEQSAVGHA
                     ITMQTPLRVFEYQKNGKFFGYAIDGTPADCVKIGMRNLMPAPPDLVVSGINHGSNTAI
                     NIIYSGTVSAAREAAIMDVPSIAISVASHQVLDFSFASKVAKMLSLEVYKRSLPKGTM
                     LNVNVPNLPEDQIAGIVVTKQGKSKWDDIYEMRIDPYGRNYYWLTGNLMDVDQSIDLD
                     QAAIKKNYVSVSPIHFDLSDYQTYEMMKEWKIENLLNGKRD"
     gene            2761..3627
                     /locus_tag="VF870_05445"
     CDS             2761..3627
                     /locus_tag="VF870_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX9251663.1"
                     /translation="MAKEIEIFQIDAFTAKAFSGNPAGVTLSSNLTEDGKQLIAKEMN
                     LSETAFISNSNIADYKLQWFTPAKEVNLCGHATIASLHFLYEKGIISNDQIISFETLS
                     GIINCAVKNGKYFMQIPLPILSKFDGCKEEVLDALGISRKDVPDLPFIILDNGYLFIC
                     VNSLDALWKIKPDFKKLNDLSDVKKEFFDIAVFTTETIEKDSSAHLRFFAPYHGIDED
                     PVTGSACGPLLLVIMELGLIDNYVDDSIIIFEQGDVLNRTGRVEVKFYKTKNELTIAG
                     NAVTVLKGKLNF"
     gene            3627..5765
                     /locus_tag="VF870_05450"
     CDS             3627..5765
                     /locus_tag="VF870_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251664.1"
                     /translation="MFGFEKIDLSFSFNTIFFFIGIIILAVYSFYAYRFTLPPVSKAK
                     RFLLTLLRTLALILLLFIFFEPVLTLTKKNILTPLNLFFFDNSKSITIDDGTDRVNTI
                     KDLIDKTKSSSLNGNKNYYSFGSKVNVINEDSLAKLDFSESFTDFSKIFADINQTEKN
                     IASITIISDGVITEGTTPIYSAEKLGIPVFTVGIGDSTKKNDVEIKNVINNEFIYAET
                     PTTILVTVINKGFEGKTSQISLYENLQLIEQKGFVLDASGVNSVSFDYTPKRSGEKKL
                     TLKISNQAGESNLSNNQKVFYIDVLSNKINVLLIAGSPSSDLTFIKNSLSADENLKVN
                     TLTQISAGNFLEQNPQAKLDSADIIYLIEFPTNITSDDFVTRLSNKLENKNTPFFMML
                     TGEVDLKKLIRIGSLLPFTVQLIDKNYLQVQPDVQVSELSNPLLENNLISEWNNLPPI
                     SQPIVSLTLKPESKLLAKVRINNQPRNNPLIVSRSFGSKRSIAVIGKDIWKWKLQTVS
                     KNLTLFDNFIFSSARWLNAPEDQKKVRIKTSKKIYSSDEVIEFSAQVYDESFNPVNDA
                     EVKLKINSRDLKEELNFTSVGGGLYEGSLSSIKNGDYIFTGNASINGKSLGEDKGSFN
                     VGDVDIEMVDSRMNYEFLYLLAVQTKGKYFAPNQYDELLTYLSRLNADSSKEKIITSE
                     LRLWSDEWFLIIIILLFAAEWFIRKRAGML"
     gene            5762..6466
                     /locus_tag="VF870_05455"
     CDS             5762..6466
                     /locus_tag="VF870_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX9251665.1"
                     /translation="MRFLGESKYLANTFFDFILPRLCCSCNSKLPPTQDTMCSNCFSD
                     IQKSTKDRLQREFDRKFSDKKFISEFYSPFVFEKDKELQHAIHGLKYDKKFAVGVFLG
                     KVLASEIRTNHVNWKFDLIIPIPLHQLKKAERGYNQSFYIAKGVGKILRVKVSDRIVK
                     RIKYTESQTTMNLNEREANILGAFKVKNKRMVSGKNILLLDDVITTGATISECGKILL
                     EAGANKIYAASIAIAD"
     gene            complement(6468..8096)
                     /gene="argS"
                     /locus_tag="VF870_05460"
     CDS             complement(6468..8096)
                     /gene="argS"
                     /locus_tag="VF870_05460"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX9251666.1"
                     /translation="MKEYLYSAFKEAESKIPELKEISISFDVPKTIEHGDLSSNAAML
                     LTKILKKSPRVIAQEIIDSLNIDSSVIVKTEIAGPGFINFFFTPVYTSKIIKEILDKK
                     ENFGKSDKYIGKRANVEFVSANPTGPLTVGHGRNAIVGDTIANLLERIGYEVDREYYF
                     NNAGRQMRVLGDSVKMRYLEILGDKINYPEDYYQGDYIKDIAKQLYTKHGDKLRDKDP
                     ESIFKETAEQEIFLDIKKSLNNLGINHKIFFNENTLYEDGKIEELLKTFKERNLSYEK
                     DGAVWLKLSELGQDEDKVIVKNTGEPTYRLPDIAYHAIKLDRGYNLLIDVFGSDHNAT
                     YPDVMAGLKAIGYDSSTVRVLIYQFVTIMDGGEVVKMSTRKANYITLDELSDEVGKDV
                     VRYFFNMRNVNSHMVFDLGVAKKQSDENPVFYLQYAYARISSILRRVKEEQLEVSISH
                     LDLLNHEDEQALLKVLHNFKEEVLSSAENLETHKLCTYLYDLAGAYHRFNRSCRILGT
                     EKNLAEARLALAYATMIVIKNGLSILGVSAPEQM"
     gene            complement(8124..8555)
                     /gene="rsfS"
                     /locus_tag="VF870_05465"
     CDS             complement(8124..8555)
                     /gene="rsfS"
                     /locus_tag="VF870_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX9251667.1"
                     /translation="MDSKLFAEKIADLIFNKKGYDVRIIDLRSLATFADFFVICSADS
                     DTQVKAIADEIDKTLRDEGIKCWHKEGYMALSWVLLDYVDVVVHVFKKEAREFYNIEK
                     LWGDAESVEIVDPELRKKTAKPKAKTAAKTKAKTTTKKAAK"
     gene            complement(8558..9052)
                     /locus_tag="VF870_05470"
     CDS             complement(8558..9052)
                     /locus_tag="VF870_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX9251668.1"
                     /translation="MNEIENPEEFTSKNPDQEKNSNLFFIIASAILAVLAVFLIYSIL
                     EKSGIMIFDNTETAKPQNVQQLIQVEVLNGCGVTGVGDALTDVLRAKGIDVVKTGNFR
                     SFDVENTFIIDRLGKTETANRVADSLNLDKRFIITEKNKSYFLDLTIVIGKDYKNYFQ
                     KSGS"
     gene            complement(9068..9580)
                     /locus_tag="VF870_05475"
     CDS             complement(9068..9580)
                     /locus_tag="VF870_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251669.1"
                     /translation="MKKHLLIIFVLLSVSINAQFKDPVFETEKPKDGIMNNSSNSLFG
                     FINPDNFSMHHSIGLSYSSFGGYGTSLATYTNSMMYKFSDNMNMQLDASFVTSPYSSL
                     GKDFQNSIQGIYISRAAFNYRPWDDFSISVQYRSIPNYLYDPFGRYNGLYGNSFNDGF
                     FGSGYHSLMY"
     gene            9723..10289
                     /locus_tag="VF870_05480"
     CDS             9723..10289
                     /locus_tag="VF870_05480"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX9251670.1"
                     /translation="MKILVIDNYDSFTFNLVQQLGKFGCKVIVKRNDSLSLVDVHQIN
                     PHKILISPGPKRPEDARISIDIIKELGSTIPILGVCLGHQAIAICFNGKVIKATKPVH
                     GITSKIIHNEKSLFKGIPQKFEVMRYHSLIVDKNSLPSILEITAQTEDGIIMGIRHKN
                     YPIQGIQFHPESILTEYGDEILSNWLRA"
BASE COUNT         3501 a         1769 c         1662 g         3449 t
ORIGIN      
        1 cactttataa actttatgat aatcgattgt ttgatttagt gaatctttct ttgctgcttc
       61 tgaaaagtcc gtataaatat ctacatctgc ttcaaattta ccaccaacac tttgttcttc
      121 actcataact ccgtgataac cgtaaaatgt tgctttttta attcttatta tactttgcat
      181 ttttcaacct tttttatcta cacaaattta agtattaaat atttaatcta actgattttc
      241 aaaccgctgt tttcggttaa aaacagcttc cagatttgca aacaacaatc ctattaacac
      301 gagtaaactt ccgattatat gctgagttgt tagagtttcg ttataaaata tccagcccag
      361 tataagggca actattggag ttataaaagc aacaagtgat agtaaaacaa catttatttt
      421 tttcatcagc caataaaacg aagtgaaggt aacaacgctg ccaaaaaatg cgagatagag
      481 aactgatata attgcagtgt tatcaatttt tattttagat atgtcttcaa gcagcaatcc
      541 aaaaactaaa ccggatatac cggcaataaa cattggaaca agattcattg ataatggatt
      601 taaatgttta cctgattttt taatcgacac tgctatccag gcttgaatta ttccgcttaa
      661 tgcaactgca accattccta taaataaagt tgataaatct gtggtgaatg aatcagaaaa
      721 tattaccact attccgatga acccaagagt cattccaaga attttaatct ttccgatcga
      781 ttcatccgga atcctgaaaa aagagaacag cgctacaaag aatggataca caccgaataa
      841 aacggatgct aatcctgaag gtacaaattg ctctgcccaa taaaccaaac caaatggaat
      901 tacaaatgat aaaaatccca ttaaaaaata taatcgaatg gattccttgt ctgtttgtaa
      961 tgatactcct ctgattttca ttaaagcaaa aacaaataac gatgctaata aaaatcgtga
     1021 gccgacagaa ataaaaggcg taagtgattc tagacttgct cttattgcca gccaggttga
     1081 accccaaata aaacaaagca atgcgtaaac taaaaatatt cttagaggag ttgatctcat
     1141 atcagccttt tattgcaatt actccaagcg atctgcctga taaatttcca tctcttcgat
     1201 aagagtgaag taatgaatta aactcaaaag tacataactc agatttttga atttgatttt
     1261 tcggaatttt aaaatcaagc agcattttat aattaattcc gcttatgtct aatataaatt
     1321 tttcgccttt cggcttaaca aatgtttgat caaacttttc agcaacctct ttcccaactt
     1381 cataatttgt ttgagaaatt gatgggccga tgtaaacaat catattttcc ggctttgata
     1441 aataatcttt ctgaagtttt agcagagttt ttaaaagaat tttttgctct gttccctgcc
     1501 agcctgaatg gacagcagca ataactccat tttttttatc ataaataaaa atgggattac
     1561 aatccgcaac agctatggct aagccaagat ttttttcact tgtaatcaaa gcatcgctgg
     1621 ctccattatc tccgctgcat ccgataacct gaacaatatc agaatgaatc tgtctttgat
     1681 atccgacagt atcaacagat aatcctatcg cctcaaaaaa ttcttttctg tttttatcaa
     1741 ctatactctt gtcatctccg acggaataag aaagattaaa gtaaaatgga ggttttgcat
     1801 tccctgcaga ttttgtgctg aacccaaata taatttccgg gtgattatta aatatgtatg
     1861 gtttaagtat gtacatttct tgtcagcaaa tttaagcaaa gtttggttac cataattgtg
     1921 cgaaagcaaa cccaaaattg agactattta tgcttagtgc tatattgctt tagtaaaata
     1981 aataattagg aataaaattg agaattttag tttcaaatga tgatgggatt gattctgttg
     2041 gaatcttttc attagtggaa gcgctacagg aaattgcaga agtaactgtg gttgcgccgc
     2101 ataaagaaca aagtgcagtc ggacatgcga taacaatgca gactccgctt agagtttttg
     2161 agtatcaaaa gaatggtaag ttttttggat atgcaattga tggcacaccg gctgattgtg
     2221 ttaaaatcgg aatgcgcaac ctgatgcctg cgccgccgga tttagtggta tccggaataa
     2281 atcatggatc aaacactgca ataaatatta tttactctgg taccgtctca gcagccagag
     2341 aagctgcaat aatggatgtt ccttcaattg caatttctgt tgcaagtcat caggtattag
     2401 atttttcatt tgcttctaaa gttgcaaaga tgctttctct tgaagtttat aagcgtagtt
     2461 tgccaaaagg aactatgtta aatgttaatg ttccaaacct gcccgaagat cagatagccg
     2521 gaattgttgt aaccaagcag ggaaaatcca agtgggatga tatttatgaa atgcgaatcg
     2581 atccatatgg aagaaattat tattggctaa ctggtaatct aatggatgtc gatcagtcga
     2641 tagatctgga tcaggccgca ataaaaaaga actacgtatc agtctcacca atacattttg
     2701 atctttctga ttatcagact tatgagatga tgaaagaatg gaaaattgag aatctgctta
     2761 atggcaaaag agattgagat ttttcaaata gatgctttta ctgctaaagc ctttagcggt
     2821 aatccggcag gagtaactct ttccagcaat cttactgaag atgggaagca gttaattgcc
     2881 aaagaaatga atctctctga aactgcattt ataagtaatt ccaacattgc agattataaa
     2941 ctccaatggt ttactccagc taaagaagtt aatttatgcg gacatgcaac cattgcatcg
     3001 cttcattttc tatatgaaaa aggaattatt tccaatgatc aaattatttc atttgaaacg
     3061 ctttccggaa ttattaattg tgctgttaaa aatggaaagt actttatgca aatccctctt
     3121 ccaattttaa gcaaatttga tggttgcaag gaggaagttt tggatgcact tggaattagt
     3181 agaaaagatg ttcccgattt accttttatt attcttgata acggttatct tttcatatgt
     3241 gttaattcat tagatgcact gtggaaaatc aaaccggact ttaaaaaatt aaatgattta
     3301 agtgatgtta aaaaagagtt ttttgatatt gccgttttta caacagagac aattgaaaaa
     3361 gattcatctg cacatttaag attttttgct ccgtaccatg gaattgatga agatcccgtt
     3421 acaggatctg cgtgtggacc tttattactt gtaataatgg aattagggtt gattgataat
     3481 tacgttgatg attcaataat aatatttgag caaggcgatg ttctaaatcg tacgggaaga
     3541 gttgaagtta agttttacaa gactaaaaac gaattaacaa ttgccggtaa cgctgtaaca
     3601 gtattaaaag gaaagttaaa tttttaatgt ttggatttga aaaaatagat ctaagttttt
     3661 catttaatac gattttcttt tttattggta tcataatatt agcagtttat tcattctatg
     3721 cttatagatt cacactacca ccggtttcaa aagcaaaaag atttttatta acactgctta
     3781 gaactcttgc attaattctt ctgcttttta tattctttga gccggtttta acattaacaa
     3841 agaaaaatat tttaactccc ttaaatcttt tcttttttga taacagcaag tcaataacga
     3901 ttgatgatgg aaccgatcgg gtaaatacga ttaaagattt gattgataaa acaaagtcca
     3961 gctcgttaaa tggaaataaa aattattatt catttggttc aaaggtcaat gtgattaatg
     4021 aggacagcct tgctaagctt gatttctctg aatcgtttac tgatttctca aagatttttg
     4081 ctgatattaa tcaaactgaa aagaatatag catcaatcac aatcatttcg gatggagtga
     4141 taacagaagg aacgactcct atttacagtg ctgaaaaact gggaataccc gtttttacag
     4201 ttggaattgg cgatagcacc aaaaagaatg acgtggaaat aaaaaatgta ataaataacg
     4261 aattcattta tgctgaaact ccgacaacaa ttttagtcac agttataaac aaaggatttg
     4321 agggaaaaac ttcacaaata tcgctctatg aaaatctgca attgattgaa caaaaaggtt
     4381 ttgtactgga tgcaagcgga gtaaactctg tttcgtttga ttacacacca aaacggagtg
     4441 gtgaaaagaa acttacttta aagattagta atcaagctgg tgaatccaat ttatcaaata
     4501 atcaaaaagt cttttacata gatgtcttaa gtaataaaat taatgtctta ctcattgcag
     4561 gcagtccgtc ttcagattta acttttataa aaaactctct ttcagctgat gaaaatttaa
     4621 aggttaacac attaactcaa atctctgcag gtaattttct tgagcaaaat ccacaagcaa
     4681 agcttgacag tgccgatatt atttatctaa ttgagtttcc aacaaatatt acgagtgacg
     4741 attttgtaac aaggttaagt aataaattag aaaacaaaaa cactcccttt ttcatgatgc
     4801 ttacaggtga agttgacttg aaaaaattaa tccgaatagg gagtctacta ccctttactg
     4861 ttcagctaat tgataaaaat tatttgcaag tgcagcccga tgttcaggtc agtgaattat
     4921 caaatccatt gttagaaaat aatttaatca gcgaatggaa taatcttccg ccaatatcac
     4981 agccgattgt ttcgcttaca cttaaacctg aaagcaagct tcttgccaaa gttagaatta
     5041 ataatcagcc tagaaataat ccgttaattg taagcagaag ttttggttca aaaagatcaa
     5101 ttgccgttat aggaaaagat atttggaaat ggaaacttca aacggtatca aaaaatttaa
     5161 cactatttga taattttatt tttagttcag cgcgctggtt gaatgccccg gaagatcaaa
     5221 aaaaggtaag aataaaaact tcaaaaaaga tttattcttc agatgaagtg attgaatttt
     5281 ctgcacaagt atatgatgaa tctttcaacc ctgtaaatga tgcagaagtt aagctaaaaa
     5341 ttaatagcag agatttaaaa gaagaactga actttacatc agtaggcgga ggattatacg
     5401 aaggcagtct ttcatccata aaaaatggag actatatttt taccggcaat gcttcaataa
     5461 atggcaaatc tcttggcgaa gataaaggtt cttttaatgt tggtgatgtt gatattgaaa
     5521 tggttgactc aagaatgaat tatgaattct tatatctgct ggcagttcaa accaagggca
     5581 agtatttcgc tccaaatcaa tacgatgagt tattgactta tctaagcaga ttaaatgctg
     5641 attcttcaaa agaaaaaata attacatcag aattaagact ttggtctgat gaatggtttc
     5701 tgataataat tattctgctt ttcgctgctg aatggtttat tagaaagcgg gcaggaatgt
     5761 tatgagattt ttgggggaat caaaatattt ggcaaatact ttttttgatt ttatcctgcc
     5821 ccgcctctgc tgttcttgta attctaaatt acctccaact caagatacga tgtgcagcaa
     5881 ttgtttttct gatattcaaa agtcaaccaa agacagatta caaagagagt ttgatagaaa
     5941 attttcagat aaaaaattca tttcagaatt ttattcacct tttgtttttg agaaagataa
     6001 agaacttcaa catgctattc atggattaaa atatgataag aagtttgcgg ttggagtttt
     6061 ccttggaaag gttctggcgt cagaaataag gacaaatcac gttaattgga aatttgattt
     6121 aataattcct atcccgctcc atcaattaaa aaaagctgaa cggggctata accaatcatt
     6181 ttatattgct aaaggtgttg gcaaaatatt aagagtaaaa gtatctgata gaattgtaaa
     6241 aagaatcaaa tacacagaat ctcaaacaac aatgaatctt aatgaacgcg aagcgaatat
     6301 tttaggcgct tttaaagtga aaaataaaag aatggttagt ggaaaaaata ttttattact
     6361 tgatgatgtt ataacaaccg gtgctacaat ttctgagtgc ggtaaaattc ttttagaagc
     6421 cggtgcaaat aaaatttatg ctgcttccat cgcaattgcg gattgattta catttgttcc
     6481 ggggcggaga ctcccaggat acttaaccca ttttttataa caatcattgt agcatacgct
     6541 aatgccagcc gagcctcagc aagatttttt tctgtaccta atattctaca ggatctatta
     6601 aatctatgat atgcaccggc taaatcgtaa agataggtgc agagtttatg cgtctctaaa
     6661 ttttcagcac tacttaaaac ttcctcttta aaattatgaa gaactttcag caaagcttgt
     6721 tcatcttcat ggtttaataa atccaaatga ctgattgaaa cctctaattg ttcttcctta
     6781 actcttctaa gaattgaact gatccgagcg taagcatatt ggagataaaa aacaggattt
     6841 tcgtctgatt gtttttttgc tactccaaga tcaaatacca tatgcgaatt aacatttctc
     6901 atattaaaaa aatacctgac aacatctttt cccacttcat cactaagttc atcgagtgtt
     6961 atataattag ctttccttgt ggacatttta accacttccc cgccatccat tattgttaca
     7021 aactggtaaa tcaatacacg tactgttgaa gaatcgtaac cgattgcttt taatccagcc
     7081 attacatctg gatatgttgc gttatgatca gaaccaaaaa cgtcgatcaa caaattatat
     7141 ccccgatcaa gctttatcgc atgatacgca atatcaggaa ggcgataagt tggttcacct
     7201 gtattcttta caattacttt atcctcatcc tgacccagtt cagaaagttt taaccaaact
     7261 gcgccatctt tttcataaga aagatttctc tctttaaatg ttttcagcag ttcttcaatc
     7321 tttccatctt cataaagtgt attttcatta aaaaatattt tatgatttat gccaagatta
     7381 ttcaaggatt tttttatgtc taaaaatatt tcctgttctg cagtttcttt aaatattgat
     7441 tccggatctt tatcacgtag tttatctccg tgtttagtat ataactgctt cgcgatatct
     7501 ttaatataat caccctgata ataatcttca ggataattta ttttatcacc taaaatctcc
     7561 aggtatctca tcttaaccga atcacccagg acacgcatct gtcttccggc attattaaaa
     7621 taatattcgc ggtcaacttc ataaccgatc cgctcaagga ggtttgcaat tgtgtcaccg
     7681 acaattgcat ttcttccgtg tccgactgtt aaaggccctg ttggatttgc agatacaaac
     7741 tcaacgttag ctcgtttgcc tatgtactta tccgactttc caaaattttc ttttttatcc
     7801 agaatctctt ttatgatttt tgaagtgtaa acgggagtaa agaaaaaatt aataaaccct
     7861 ggtcctgcaa tttctgtttt cacaataact gaactatcaa tattaagtga atcaattatt
     7921 tcttgcgcaa ttactctggg acttttctta agaatcttag taagaagcat agcagcgttg
     7981 cttgacaaat caccatgctc tatagttttg ggtacatcga aagaaataga aatttctttt
     8041 agttctggaa ttttactttc ggcttcttta aatgcagagt agaggtattc tttcaaatga
     8101 tataaactta aattagtgaa taattatttt gcagcttttt tagttgttgt ttttgctttt
     8161 gttttagcag ctgttttggc ttttggtttt gctgtttttt ttcttaattc aggatctaca
     8221 atttcaacag attcagcgtc gccccacagt ttttcaatat tataaaactc tctggcctct
     8281 ttcttaaaca cgtgcacaac aacatcaaca taatccagaa gaacccagct taatgccata
     8341 tatccttctt tgtgccagca cttaattcct tcgtctctta aagttttatc gatctcatca
     8401 gcaattgctt ttacctgtgt atcagaatcc gcagaacata tcacaaaaaa atccgcaaat
     8461 gtagcaagac ttcttaagtc tattatacga acatcgtatc cttttttatt aaaaataaga
     8521 tcagcaattt tttcagcaaa taattttgaa tccaatatta actcccgctt ttttgaaaat
     8581 aatttttata gtcttttcca attacaattg taaggtctaa aaagtaactt ttgttttttt
     8641 ctgttatgat aaatctttta tcaagattta atgaatcagc tacacggttt gcagtttctg
     8701 tttttccaag ccgatcaatt atgaaggtat tttcaacatc aaatgaacga aaattaccgg
     8761 tttttacaac atcaatacct tttgctctta aaacatctgt taatgcatca ccaacacctg
     8821 taacgccgca tccgtttaaa acttctacct gaatcagctg ttgaacattt tgcggcttag
     8881 cagtctcagt gttatcaaat atcattattc ctgatttttc caaaatagaa taaataagaa
     8941 atactgctaa cactgctaag attgctgatg caataataaa gaaaaggttt gaattttttt
     9001 cctggtctgg attttttgat gtgaattctt ccgggttttc tatttcattc actatgcttc
     9061 ctgaaaatta atacattaat gaatgatagc cggaaccgaa aaatccatca ttgaatgagt
     9121 taccgtataa accattgtat ctgccgaaag gatcatataa ataattggga atgcttctat
     9181 actgcactga tattgaaaaa tcatcccagg gacggtaatt gaatgcagca cggcttatat
     9241 aaattccctg aattgaattt tgaaaatctt tgcctaagga gctataagga cttgtaacga
     9301 aacttgcatc aagctgcata ttcatattat ctgaaaattt atacatcata ctgtttgtat
     9361 aagttgctaa acttgttccg tatccgccaa aggaagaata ggataatcct attgaatgat
     9421 gcatgctaaa attatcagga ttaataaatc caaataagga attagatgaa ttgttcatta
     9481 ttccatcttt tggtttctca gtttcaaata ccggatcttt aaactgagca ttgatagaaa
     9541 ccgacaacaa tacaaatatt ataagtagat gctttttcat aattttctca aaatcttcac
     9601 ttcgatttta tacaaatcct tacttaatgt caatgaatgt taatgtactt tagactcctc
     9661 aaactgaatt tttgcacatt gtttagagat tatttctcaa aatattttct ttattttgaa
     9721 ctatgaaaat tttagtgata gataattacg attcctttac ttttaatctg gttcagcagc
     9781 tcggtaaatt tggttgtaaa gtcatcgtaa aaagaaacga ctctttatcg ctagttgatg
     9841 tacatcaaat aaaccctcat aaaattctta tatcacccgg accaaaaaga cccgaagatg
     9901 caagaatttc aatagatata attaaggaac ttggttctac aatcccaatt ctcggagtct
     9961 gtcttggtca tcaggcaatt gcaatctgtt tcaatggcaa agtaattaaa gctacaaaac
    10021 cggtccatgg gataacatca aaaataattc acaatgaaaa atctcttttt aaggggattc
    10081 cacaaaaatt cgaagtaatg agatatcatt cattaattgt tgataaaaat tctctgcctt
    10141 caatcttaga aatcactgca caaacagaag atggcataat catgggaatc cgtcataaaa
    10201 attatcccat tcaaggtatt caatttcatc ctgaatcaat attaactgaa tacggagatg
    10261 aaattttatc taactggctg cgagcataaa aaaatcaaaa acaaattatt caactctaac
    10321 tatcatttca atctcaacgg aagcatcaag aggcagagcc gtaactccaa ctgcactgcg
    10381 t
//
LOCUS       DASWBY010000293         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_724384, whole genome shotgun sequence.
ACCESSION   DASWBY010000293 DASWBY010000000
VERSION     DASWBY010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724384"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /gene="htpX"
                     /locus_tag="VF870_05485"
     CDS             complement(<1..835)
                     /gene="htpX"
                     /locus_tag="VF870_05485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX9251671.1"
                     /translation="MNGLKTVFLMSAMMALFLLVGYAIGGKSGMTIAFVFSLVMNFGS
                     YWFSDKIVLAMYKAQPITREQSPKFYDMIEQLAQNANLPMPKVYIINDPTPNAFATGR
                     NPQNAAVAATTGILQGLNNDELAGVMAHELAHVKHRDILISTIAATLVGTISYIAQMA
                     GWAAMFGRSDDREGGGLGGLFLIILSPIIAMLLQMAISRSREFSADAGGAEISGNPMA
                     LASALQKISRGNQVSHLRNTNPSTAHMFIISPLFGGMGKLFSTHPPVEQRIAKLQEIA
                     AG"
     gene            1093..>1686
                     /locus_tag="VF870_05490"
     CDS             1093..>1686
                     /locus_tag="VF870_05490"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9251672.1"
                     /translation="MNKVVFLALTIIMFGNFSYGQNTYDFLRLDGSARAGALGGSFVS
                     NNDDADVIFYNPAGIDLLQGNPASFSFVKHLMDINLATLSYSTEFEDVGRFGAAIKYI
                     NYGSFEGADDFGTKTGEFSAGEFAFVLGYANQLDDNFYYGANAKFIYSSIETRYSTAM
                     AVDLGLHYAIPDKNWNFGFAVLNLGGQISKYYNTTEDL"
BASE COUNT          524 a          326 c          289 g          547 t
ORIGIN      
        1 tacccgcagc aatttcctga agctttgcta ttctttgctc aaccggagga tgagtagaaa
       61 ataatttacc cattccacca aacaaaggac taataataaa catatgagcc gttgaaggat
      121 ttgtattcct taaatgacta acctgatttc ctcttgaaat tttttgcaaa gcagaagcca
      181 gcgccattgg attgccgctt atttccgcac cacctgcatc tgcagaaaac tcacgcgatc
      241 ttgaaattgc catctgcaga agcattgcaa ttattggaga aagaataatt aaaaataatc
      301 cgcctaaccc tcctccttct ctatcatcgc ttctgccgaa cattgctgcc catccagcca
      361 tttgtgcaat gtaagaaatt gttcctacta aagttgcagc aatagtactg attaaaatat
      421 ctctatgttt aacgtgcgca agttcgtgtg ccattactcc ggcaagttca tcattattta
      481 atccctgcag aattcccgtt gtagcagcaa ctgcagcatt ttgtggattt cttcctgtag
      541 caaatgcatt aggagtcgga tcatttatga tataaacttt tggcattggc agattagcat
      601 tttgtgccag ttgttcaatc atatcataaa atttgggtga ttgttctctg gtaatcggct
      661 gagctttata cattgccaga acgattttat ccgaaaacca atacgaacca aaattcataa
      721 ctaaggaaaa cacaaaagca atagtcatac ccgattttcc accaattgcg taaccaacaa
      781 gcagaaaaag agccatcatt gcagacatta gaaatacagt tttaagtcca ttcattttta
      841 cattttctcc gtttttatag ttcaaagtta tattaccaaa attgatgaat caagttaaac
      901 aggattcgcc tgttttagat gagataatca tcactaaagt tgcaaaaaat ttgattactc
      961 ttttgtaaca ttaattcata aaatacagtt caattctaaa tcctttactc aaaattctta
     1021 ataaagaaaa atatttcccc ttgcctttat tttgcgatat agttaaatag acacagaaat
     1081 tttggagaat taatgaacaa ggtcgtcttt ttagctctta ccataataat gtttggcaat
     1141 ttttcttacg gccaaaacac ttatgatttt cttcgtcttg acggaagtgc acgtgcaggt
     1201 gcccttggcg gcagtttcgt ttccaataac gatgatgctg atgttatttt ttataatcct
     1261 gcaggtatag atttactcca gggaaatccg gcctcattct ccttcgtaaa acatttaatg
     1321 gatataaatc tcgcaacact ctcttactca actgaatttg aagacgttgg aagatttggt
     1381 gcggcaatca aatatattaa ctacggcagt tttgagggtg cagatgactt tggaacaaaa
     1441 acaggtgaat ttagtgcagg tgaatttgct tttgtactcg gttatgccaa tcagcttgat
     1501 gataattttt attatggtgc taatgctaag tttatttatt ccagtataga aacccgttac
     1561 tccacagcaa tggcagttga tttaggtttg cactatgcaa tacctgataa gaattggaat
     1621 tttggttttg cagtgttaaa ccttggcgga caaatttcaa aatattacaa cacaacagag
     1681 gatttg
//
LOCUS       DASWBY010000294         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_423458, whole genome shotgun sequence.
ACCESSION   DASWBY010000294 DASWBY010000000
VERSION     DASWBY010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423458"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..336)
                     /locus_tag="VF870_05495"
     CDS             complement(1..336)
                     /locus_tag="VF870_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9251673.1"
                     /translation="MKNILIYLGLVITIVALYGFAFTIADQPGDDGKTIFVNNKCNMC
                     HTVSSAGIESKKSDAVDLYTVGKDKTVEFLTKYLKKEAKLNDKDHKASFKGTDEELTK
                     LVEWLSTLK"
     gene            complement(632..991)
                     /locus_tag="VF870_05500"
     CDS             complement(632..991)
                     /locus_tag="VF870_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251674.1"
                     /translation="MKNIVIYSPDFSLCYSLLMYLQTQYKVVATTDMEVVSSLICSGS
                     ADLVIMDTEPNLELLTKCEQFKKCKSEVPLILTYVFNNRIKESEGKIKKFVNEIFYKP
                     FDLNEISSKIPSLLPSI"
     gene            complement(1210..>1647)
                     /locus_tag="VF870_05505"
     CDS             complement(1210..>1647)
                     /locus_tag="VF870_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9251675.1"
                     /translation="VDIHLPSLKEREEDIPLLADHFLNKYRKELNKNIKGIDNEAMRA
                     LLSHEWKGEVRELENIIERAVIFCKGDMITLQELPASFKPKMDELDFSFNGSLDDSVR
                     KFERDFILRALENNDNNKEKTADALRVGLSTLYRKLKELDIKV"
BASE COUNT          526 a          262 c          237 g          622 t
ORIGIN      
        1 ctatttaaga gttgataacc actcaaccaa ttttgtaagt tcttcgtcag ttcctttaaa
       61 tgaagcttta tgatctttgt catttaactt agcttctttt ttcaagtact tcgttaagaa
      121 ctcaacagtt ttatcttttc caacagtata taaatctacc gcatcagatt ttttagattc
      181 gattccggca gatgaaactg tatgacacat attacattta ttatttacaa aaattgtttt
      241 accgtcatcg cctggctgat ctgcaatagt aaaagcaaat ccatataaag caactattgt
      301 tattaccaat ccaagataaa tcaatatatt tttcatgttt ttagatcctt ttaatttgaa
      361 ttttttagtt attgattttg tgcccttatg ttacaattag gataccactc ttttaatgtg
      421 aattatttgc taaaaatcca atattagctt tgttcagatt tgcctaattt ctcagctttg
      481 ggaagtcttt ttctgacata ttcgcaagaa ttggaatgat ttctaaaatt attgcgcggg
      541 taaactaatt gaatttgatg tattaaaaat tatactaact aaatgattta atgaacactg
      601 gaagttgtta taaaaattct aattcaaatt attaaatact gggcagcaga gaagggattt
      661 ttgaagaaat ttcattaaga tcaaatggct tgtaaaaaat ttcatttaca aacttcttaa
      721 tttttccttc ggattcttta attctattgt taaagacata agttagaatt agaggtactt
      781 ctgatttaca ttttttaaat tgttcgcatt tggtaagaag ttctagattt ggttcggtat
      841 ccataataac taaatctgca gaaccactgc agattaatga agagacaact tccatatcag
      901 ttgtggcaac aaccttatat tgtgtttgca agtacattag taagctataa cacaaactga
      961 aatcggggct atatataact atatttttca tttactttat tgtgatactt ttcccctgaa
     1021 tggaaaagca ggtataaatt tgctatttgt gtgccaaaat tgattgtgga gatattgtgc
     1081 gggtaatggt tggtttttgg aattattgcc taaaaaagct tattgttcta ttctcgcaat
     1141 tgaaaatcaa taaagttgca ttttaattat tgagaatata tccaaataag tgcaaagtgc
     1201 tttacaatat taaaccttaa tatccagttc ttttaatttt cgatacaaag tagataagcc
     1261 cacccttaaa gcatcggcag ttttttcttt attgttatcg ttattctcga gcgcccttaa
     1321 aataaaatcg cgttcaaact ttcttacaga atcatcaagc gaaccattaa acgagaaatc
     1381 taattcatcc attttaggtt taaatgatgc cggaagttcc tgcaatgtaa tcatatctcc
     1441 tttgcaaaaa attacggcac gttcaataat attttctaac tctcttactt cacctttcca
     1501 ttcgtgactc aataaagctc tcatagcttc gttatcaata cccttgatgt tcttgtttaa
     1561 ttcttttctg tacttgttaa gaaaatgatc agcaagtaaa ggtatatctt cttctctttc
     1621 tttaagagat ggaagatgga tatcaac
//
LOCUS       DASWBY010000295         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_611019, whole genome shotgun sequence.
ACCESSION   DASWBY010000295 DASWBY010000000
VERSION     DASWBY010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611019"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VF870_05510"
     CDS             <1..723
                     /locus_tag="VF870_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX9251676.1"
                     /translation="RNGLVSFGGFILNSGKSLLPVSIGRKRKIEFIGDSFVAGLGSEG
                     KTRDCVFNRDNENSYIAFGPVLARRLNADYSIIAVSGIGIIRNFGDTTFTSPKTLPHY
                     FERTCFNEDLKWDFKKWQPDLVVVRLGNNDYWHKPYPVTSEFENAYINFLKQIRKFYP
                     NTSMLVLCEPVRKDPHCDFIKNVVAEFKSKLKDKKIWYKELDVNLDKTKDYGCQEHPN
                     ESGHKKIADALEPIVRRAMGWR"
     gene            858..>1509
                     /locus_tag="VF870_05515"
     CDS             858..>1509
                     /locus_tag="VF870_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251677.1"
                     /translation="MSKLFSPLKIRNVELKNRIAVSPMCQYSCVDGVMTDWHLVHLGS
                     RAVGGAGLIFTEATAVSPEGRISPDDAGIWNDEQVQFLKRITSFIKSQNSVPGIQLAH
                     AGRKASTFSPWKGSGEVKIQNGGWKTLAPSKLQFAENFPDPKEMDEKDIKLVIDQFVN
                     AAIRSIDAGFEVIELHMAHGYLVHEFLSPLSNQRKDNYGGILENRCRIAIEIANSVR"
BASE COUNT          526 a          223 c          296 g          464 t
ORIGIN      
        1 agaaatggtt tagtttcatt tggtggattt attttaaatt ctggaaaaag tttattgcct
       61 gtaagtattg gaaggaaaag gaaaattgaa tttattggag attcgtttgt tgctggatta
      121 ggaagcgaag gtaaaacccg ggactgtgtt tttaacagag acaatgaaaa cagctacata
      181 gcttttggac cagtccttgc cagaagatta aatgcagatt attccataat tgcagtctca
      241 ggaatcggca taattagaaa ttttggcgat acgacattta catccccaaa aactttacca
      301 cattactttg aaagaacttg tttcaatgaa gatctgaaat gggattttaa aaaatggcag
      361 ccggaccttg ttgtagtaag attaggtaat aatgattatt ggcataaacc atatcctgtt
      421 acaagcgaat ttgaaaacgc atacattaat ttcttaaaac aaatccggaa attttatcca
      481 aacacaagta tgcttgtttt atgtgaaccc gtcagaaaag atcctcattg cgattttatt
      541 aaaaatgttg tcgctgaatt caagagtaaa ctaaaggata aaaaaatttg gtataaagaa
      601 ttagatgtta atttggataa gaccaaggat tatggttgtc aggaacatcc aaatgaatca
      661 ggacacaaaa agatagcaga tgcacttgaa ccaatagtcc gcagggcaat gggctggaga
      721 taatactctc catgttttag tatatttttt tcagtttaaa tgtttcattc aataatcatt
      781 gatgaatgcg agtaatattt tttttaagtt gcaattatta aaaactatta gttagtaaat
      841 ctaaaccaga aaattatatg agtaaattat tctcacctct aaaaatcagg aatgttgaat
      901 taaaaaatcg aattgcagtt tcaccaatgt gtcagtattc ctgcgttgat ggtgttatga
      961 ctgactggca tcttgttcat ttaggcagcc gtgctgttgg cggtgcggga cttattttta
     1021 ctgaagctac agcagtatcg cccgaaggaa gaatttctcc tgatgatgca ggcatttgga
     1081 acgatgaaca agtacaattt ttaaaaagaa ttacatcgtt tataaaatca cagaactctg
     1141 ttccaggtat tcaattagca cacgccggta gaaaagcatc tactttttct ccatggaagg
     1201 gcagtggaga agttaaaata caaaacggag gatggaaaac tttagctccg agcaaattac
     1261 aattcgctga aaattttcct gatccaaaag aaatggatga aaaagatatt aaacttgtta
     1321 ttgatcaatt tgttaatgca gctataagaa gcattgatgc aggatttgaa gtaatcgagt
     1381 tgcatatggc acatggttat ttggttcacg aatttctatc acctctttca aatcaaagaa
     1441 aagataatta cggaggaatc cttgagaaca gatgccgaat tgcaattgag attgcaaact
     1501 cggttagaa
//
LOCUS       DASWBY010000296         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_47404, whole genome shotgun sequence.
ACCESSION   DASWBY010000296 DASWBY010000000
VERSION     DASWBY010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47404"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..>1756
                     /locus_tag="VF870_05520"
     CDS             61..>1756
                     /locus_tag="VF870_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9251678.1"
                     /translation="MFEKAMERNNIVITTSTASGKTLSFLLPVIQEILSNPLARAIFI
                     YPTKALASDQYRAILPYLEYFGDNRISAGVYDGDTTVNERSRIRKSANIILTNPEMIN
                     AAFLANHSKFGFDFIFSNLKYVVIDELHTYRGAFGSHLANVFRRLGRVCRYYNSSPQY
                     LCSSATIANPVELAEEICGQKFIRVDRDGSPAPQKKYMLVQPPKIMGPDKKYYGQVQS
                     TSVAADLIPDLVENDNSFIAFAKSRRNVEVVLKESRDKLEMENFFGASLSDKISGYRG
                     GYTPLERKEIENKMITGVLSGLVSTNALELGIDIGKIDTTVIVGYPGTRASFWQQTGR
                     AGRSGKESTNYLILDNLPFDQYIAINPDWLFENGSENAVMDKNNLLIELAHIRAAAAE
                     IPLTLDDISIFPDLGETIPVLIRASELTNQSGKFAWSGNSFPAGDFSLRNIDKSRYKL
                     INKENNKEITEMDEMQAFREIHNGAIYMHDGVQYQVIKLDLESRTAFAIAFNGNYYTM
                     PGGITNIRIIQGGKEMDYKRVEVAFGDVNVDEIVYMFKKLQFHNHQNLGFEQLEKPL"
BASE COUNT          627 a          254 c          354 g          521 t
ORIGIN      
        1 gaattatgcg gatatctttc tcaaaatggg attatgaagt tatattgtca tcaagcagaa
       61 atgtttgaaa aagcaatgga gagaaataat attgtgataa ccacatctac cgcaagtgga
      121 aaaaccctta gctttttgct cccggttatc caagaaattt tatcaaatcc tcttgcaaga
      181 gcaattttta tatatcctac aaaagccctt gcaagcgatc agtatcgtgc tatcttacca
      241 tacttagaat acttcggaga taacaggatt tcagcaggtg tatatgatgg agatacgact
      301 gtaaatgaaa gaagcagaat tagaaaaagt gccaatatta ttttaaccaa tccggaaatg
      361 ataaatgctg catttttggc gaatcacagt aaatttgggt tcgactttat attttctaat
      421 ctgaaatacg tagtaattga tgagttgcat acttatagag gtgcattcgg ttcccacctg
      481 gcaaatgtct ttcgaagact gggaagagta tgcagatatt ataactcatc accacaatat
      541 ctatgcagtt cagcaaccat agcaaatcct gttgaattag cagaagaaat ctgtggacag
      601 aaatttatcc gagttgatag ggatggttcg ccagcaccgc agaaaaaata catgcttgta
      661 caaccgccca aaattatggg ccctgataaa aaatattatg gtcaagtaca atcaacctca
      721 gttgctgctg atttaatacc tgatttagtg gaaaatgata atagttttat tgcttttgct
      781 aaatcacgaa gaaatgtcga agtagtatta aaagaatcaa gagataaatt agagatggaa
      841 aacttttttg gagcatcttt atcagataag atatctggat ataggggagg ctatacgcca
      901 cttgaacgta aagaaattga aaataagatg attacgggag ttttgagtgg attagtatct
      961 acaaatgctt tagagctagg gatagatatt ggaaaaattg atacaacagt aattgttgga
     1021 tacccaggaa caagagcatc tttttggcag cagacaggta gagcaggtag aagtggaaaa
     1081 gagtctacaa attatttgat tctagataat ctaccttttg atcagtatat agcaattaat
     1141 ccagactggc tctttgaaaa cggaagtgaa aatgcagtta tggataagaa taatctatta
     1201 atagaactgg ctcacatacg tgcagcagct gctgaaatac ctttaacctt agatgatatt
     1261 tcgatttttc cagacctcgg agaaacaatt cctgttttaa ttagagcaag tgaattaacc
     1321 aatcagagtg gaaagtttgc atggagtggt aattcatttc cagcaggaga ttttagttta
     1381 agaaatattg ataaatccag atataaactg attaataagg aaaacaataa agaaattaca
     1441 gaaatggatg aaatgcaggc cttccgtgaa attcataatg gagcaattta tatgcatgat
     1501 ggtgttcagt atcaggttat aaaacttgat ttggaaagta gaactgcatt tgcgattgcc
     1561 tttaatggaa attattacac aatgcctgga ggcattacaa atataagaat tattcaaggt
     1621 ggtaaggaaa tggattataa gcgtgtcgaa gttgcttttg gtgatgtaaa tgtggatgaa
     1681 atagtttata tgtttaaaaa actacaattt cacaatcatc aaaatttggg atttgagcag
     1741 ttagaaaaac ccttat
//
LOCUS       DASWBY010000297         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_13229, whole genome shotgun sequence.
ACCESSION   DASWBY010000297 DASWBY010000000
VERSION     DASWBY010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13229"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VF870_05525"
     CDS             complement(<1..630)
                     /locus_tag="VF870_05525"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX9251679.1"
                     /translation="MKFLFLFICLFTTSNFTSIYSQIFSEEPVLRVRIIYTVDTLNLS
                     FLDEWNMSLSDSQQNLKIAKNDSLLIFIQNDSIALNNYSEKEISYYKNILLKTVDSAG
                     TLKIKNVPYGIGWWWQGTEDRIYEGDIEISINENNKLDVIVSLPLEKYLCGVVPYEIG
                     NDAPLEALKAQVIAARSETVSAWISGKYRGSNFDLCADVECQVFAGNNKR"
     gene            complement(765..>1857)
                     /locus_tag="VF870_05530"
     CDS             complement(765..>1857)
                     /locus_tag="VF870_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9251680.1"
                     /translation="FANMSHELRTPMMGILGFSEVLMQELKDYPDYLRMVNSINSSGQ
                     RLLETLNLILNLSKLEASKVNVELKAQNIIPVLKECFESFESAAAKKNIHYTFISEYP
                     EILCNIDKLLLTSIFNNLLNNAIKFTDSGNVTLSISIDSDFAVVSVTDTGIGISEAKQ
                     NLIWEEFRQASEGYNRGFEGTGLGLTIAKKYTDLMKGSITVKSSFGKGTTFKVSFPLS
                     LSIDSKINMDNIEKTSTAKMQSTSKILYVEDDEISIKYVKTVTKSLYTIDSASDSDEA
                     LNKINQTKYDAVLMDINLRRGMDGLELTKVIRKIDGYQSTPIVAITAFAMGHEKEEFL
                     AKGMTHYLSKPFVKNQLLNLLADIVDKKS"
BASE COUNT          590 a          324 c          267 g          676 t
ORIGIN      
        1 tcttttatta ttgcctgcaa aaacctgaca ttcgacatca gcgcaaagat caaaattgct
       61 tcctctatat ttacctgaga tccatgcaga aacggtttca gatctggcag cgataacttg
      121 agctttaagt gcttcaagag gtgcgtcatt accaatttca taaggaacga caccgcataa
      181 atatttttct aaaggaagcg acacgattac gtcaagctta ttattctcat ttattgaaat
      241 ctcaatatca ccttcataaa tgcggtcttc agttccttgc caccaccatc caattccata
      301 aggaacattt ttaatcttta aagtgccagc agaatcaact gtttttaata agatattttt
      361 ataataactt atttcctttt ctgaataatt atttaatgct attgaatcat tttgaataaa
      421 tattaataaa gaatcattct tagcgatttt taaattttgt tgtgagtcgg atagagacat
      481 attccattca tctaaaaaac ttagattgag agtatcgaca gtatagatta ttctgactct
      541 tagaacaggt tcttctgaaa atatttgtga atagatcgaa gtgaaatttg aagttgtaaa
      601 taagcagatg aataaaaaaa ggaatttcaa tttatatccc tttaactgta aagtgtttcc
      661 taaaagccaa aatcagcgtg ctgaatttat aagaattaaa ataaactttc gttgttataa
      721 tactttagtt ttaattgtga tatttaacca gtaatagttt tctgctaact ttttttatca
      781 acaatatctg ctaacagatt aagcagctga ttctttacaa atggtttcga taaataatgt
      841 gtcattccct ttgccaggaa ttcctctttt tcatgtccca tagcaaatgc agtaatagca
      901 acaattggtg tcgattgata tccatctatt tttcgtatta cttttgtaag ttcaagtcca
      961 tccatacctc gtcgcagatt tatatccatt aaaacagcat cgtacttagt ctgatttatt
     1021 ttattcaaag cctcatcgct gtctgatgca gaatctattg tatataaaga ttttgtaaca
     1081 gtctttacat actttatgga aatttcatca tcttcaacat aaagaatttt ggaagttgac
     1141 tgcatttttg ccgttgaagt cttctctata ttatccatat ttattttgct gtcaatactt
     1201 aaagacaatg gaaagcttac tttgaatgtt gtgcctttac caaatgaact cttaactgta
     1261 attgaaccct tcattaaatc tgtatacttt ttagcaattg ttaatccaag tcccgtacct
     1321 tcaaatccac ggttataacc ttcgctggct tgcctgaatt cttcccagat taaattttgc
     1381 ttagcttccg aaataccaat tcccgtatct gttactgata ccacagcgaa atctgaatct
     1441 atggatatac ttaatgtaac gtttccggaa tctgtaaact taattgcatt attcaataga
     1501 ttattaaaaa tacttgttaa aagaagctta tcaatattgc acaatatttc cggatattct
     1561 gagatgaatg tatagtgaat attttttttc gctgcagcag attcaaaaga ttcaaagcac
     1621 tcttttaaaa ccggaataat gttttgagct ttaagttcaa catttacttt tgaggcttcc
     1681 agcttggata aatttaaaat aagatttaat gtttcaagta atctttgtcc tgatgaatta
     1741 attgaattta ccatgcgtag ataatccgga taatctttta gttcctgcat aagtacttca
     1801 ctgaatccaa gtatgcccat cattggtgtt cttaactcat ggctcatgtt cgcaaag
//
LOCUS       DASWBY010000298        14052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_724668, whole genome shotgun sequence.
ACCESSION   DASWBY010000298 DASWBY010000000
VERSION     DASWBY010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 14052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14052
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724668"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VF870_05535"
     CDS             <1..478
                     /locus_tag="VF870_05535"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX9251681.1"
                     /translation="EKEKLYAINDIVIDKGGWPKMIELTVWVDGEYVTTFSADGLIVA
                     TPTGSTGYSISVGGPIVSPKTDVITLSPISPHSLTVRPLVLPSSQEIVIKADSPHKEI
                     QVSCDGQRVFDFPPPTEIKICKSDRPLKLVHTSLTTYFETLRNKLLWGIDLRIKKD"
     gene            746..1249
                     /locus_tag="VF870_05540"
     CDS             746..1249
                     /locus_tag="VF870_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9251682.1"
                     /translation="MLKYLIPLAIIITAIQIYSQDYKIIPDEKNGEPLILGYCPASAM
                     NDSVFDGTWSTEYNNYQPDFETLDSLEGKLEDVTIKIVFRSTCSDSREQLPRLFKILN
                     ELNYDVNTITLIGVNREKQGLSNETEGLEIEFVPTIIFYKNGSEIGRIIETPVESLEK
                     GFVEDRY"
     gene            complement(1302..2486)
                     /locus_tag="VF870_05545"
     CDS             complement(1302..2486)
                     /locus_tag="VF870_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEX9251683.1"
                     /translation="MELWQQMVRDSVHTVDQLVEKFGIDRKVAEELDEFFQARINPYY
                     LSLIRHPGDPIWRQCVPDKAELEDFDAAEDPLMEDAMSPVPNITHRYPDRALFLVTSQ
                     CGLYCRFCTRKRKVGDYEKISMKGLESAFKYLEEHTEIRDVILSGGDPLMLTDAMLEK
                     ILQRIRAIPHIEIIRLGSKMPCVLPHRITTKLVNMLKKYHPIYCNTHFNHPWEITPES
                     SKACQMMADAGIPMGNQMVIMKGVNDDPAVVKELMQKLLKIRVRPYYMYMADETKGAN
                     HFRTSIETGIKILEGLRGHTSGLAIPHFVIDAPGGGGKIPIIPNYVLHKDEERIILRN
                     FQHKIYTYKNYADANNPEGFGSKSKSNGKNGSNGNGNGKGKKDDIKPKKIKLPELSEI
                     EN"
     gene            complement(2496..2978)
                     /locus_tag="VF870_05550"
     CDS             complement(2496..2978)
                     /locus_tag="VF870_05550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9251684.1"
                     /translation="MIRRIKPSDEVAIENMLTRIPNFNEEEIKVAMELVNISVMNPDQ
                     TDYHLYVYENDGKIVGYHCTGKRPLTDGVYDLYWIASDPATSGKGIGKQLLEHAEEFV
                     RSNNGRWLLAETSSKESYASTQNFYLRNKYSIISEIRDFYSVGDDLLVFGKYFNNKNH
                     "
     gene            complement(2953..4014)
                     /locus_tag="VF870_05555"
     CDS             complement(2953..4014)
                     /locus_tag="VF870_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9251685.1"
                     /translation="MKTEAKILICYNSPVSVFSIYNGKPAEENSDSKDLSEHSFASNM
                     VEIENHLKTFFTDVKSLPIDKNVTRTIAEITSYNPDAILNFVESVEGITYYEYCIAGL
                     YELLGYQYTGNVPQTLGNCLDKKKAKEILYSNGINTPRAITLTPKQKFTKKDVNLTYP
                     LILKLLDEDASIGISENSVVNSYRELKKHFTFLTETYNKSLIVEEYIVGRELNCAVLG
                     DKPLPISEIDFTGLPDGLPKIVTYDGKWIEGSTYYNFTKPVCPAKIDSDIQKKVEELA
                     KKAYKALGCRDYARVDVRLSNDGIPYVIEINPNPDVSSDSGFARAAAASGKSYSELLF
                     AITSFALQRKYNDSKNKAV"
     gene            complement(4011..5066)
                     /locus_tag="VF870_05560"
     CDS             complement(4011..5066)
                     /locus_tag="VF870_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9251686.1"
                     /translation="MNVALAYNLKPESESLSLSVSPIVNNHSQSLQAQMDTYAEWDTW
                     ETINAVKDAISLYNNVTLIEADEEAYNKFRQVKPDIVFNIAEGAFGVSREAQIPAMLD
                     MLQIPYTGSDALTLAICLDKARTKEILSYYKVPTAKFFVADRIDDAENHNLDFPLIVK
                     PISEGSGKGIYASSFVHNKEELKREIKRITSEYNQSALIEEFLSGREFTVAMLGNNGD
                     LKILPAIEMRYDKYPEGTAPLYSYEAKWIFDTKENEFDVFDCPANITKELEQKINKVC
                     TDTYRVLKCRDWSRIDLRLDKNGEPNIIEINPLPGIIPDPNENSSFPKAARAAGIEYN
                     DMINTVLASAIKRYNLL"
     gene            complement(5313..7520)
                     /gene="recG"
                     /locus_tag="VF870_05565"
     CDS             complement(5313..7520)
                     /gene="recG"
                     /locus_tag="VF870_05565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX9251687.1"
                     /translation="MKSDKENILESSAQYIKSVGPKRAESFSKIGINTIRDMIFYFPS
                     RHLDRTTVLNAAKAYGYAMNGYDGEVTIIGKVADKEKRNLGRKEILKVQFRDSSGFIE
                     CVWFHGVKYFHSVFNEGDYFAISGKPEINYNKLQIVHPDFDKITEEESQSFLNTGKII
                     PFYRIPKELKQKNIGDLSLRRIINTAVESYVDNLEETLPQKIIDRHKLIDLITAVKNY
                     HYPESKELFEQAKRRFKFEEMFYIELLVAIRKNNYQTKLLGNKMEIKTNLVSDFIKTL
                     PFELTKSQLSVLSEIKIDMLAEKPMNRLLQGDVGSGKTIVALISMLIAIDNGFQTALM
                     APTEILADQHAKNISVMMSKLSSITKRKEIKVSLLLGGQKKSVRNQKLSDIELQEADI
                     IIGTHAIFEEQVKFNNLGLIVIDEQHRFGVKQRALLQSKGKTPDVLVMSATPIPRTLS
                     MTVYADLDLSVINEMPKNRIPIKTVLRGEKKLPEIYNFIIDKAKEGYQTFLVYPLVED
                     SEKLDLKAAITFYEDLKENHLKNLKLGLIHGRMNWQEKEEVMLMFQKKMFDVLVSTTV
                     IEVGIDIPDANIILINDAHRFGLSQLHQLRGRVGRSSKQAYCILITKDEIAAKQNANE
                     LELDYLSSVQLEKYKSSVRLQSMVKYLDGFKIAEIDLKLRGPGDIFGTKQSGFPDLKY
                     TNIVEDNELILEAKQSAFKLIEVDPKLEKNFSALIRKNLIKHYSENLSYAKIA"
     gene            7622..8998
                     /locus_tag="VF870_05570"
     CDS             7622..8998
                     /locus_tag="VF870_05570"
                     /inference="COORDINATES: protein motif:HMM:NF002205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX9251688.1"
                     /translation="MDWKLLLTDDRLGIEKSHSAKKDGRSQFQKDFDRIVFSPAFRRL
                     QDKTQVFPLPESDFVHTRLTHSLEVSVVGRSLGNLVGERIIAKHPKLKKDFTQFHFGE
                     IVAAACLAHDIGNPPFGHSGEDAIAEYFKNGNGKKFKEEIVDEKKWNDLIRFEGNAQG
                     FRIITKLQNQDVDGGLQLTYATLSALNKYPKESLTDLEIKHATGHKNIYKKFGFFQSE
                     KEIYKHVAEKTGLTKPVTSEKYLWHRHPLSFLVEAADDICYSIMDLEDGFRLGLLTFK
                     ETENLLLPLIHKRDLSGYKKRDENEKVGYLRAKAISDLVNLVADAFMENEKDILEGNF
                     NKELVTVISKSNTLMKIEKASIAKIYRHRSVVEREVAGYEVLGGLLDTFITAYNERSS
                     DNLTPKNRAIINLIPKRFFFAEKNNPELYQRLLRVIDFISGMTDSYAVSIFRKIRGIS
                     LPGGRVTE"
     gene            complement(9139..10140)
                     /locus_tag="VF870_05575"
     CDS             complement(9139..10140)
                     /locus_tag="VF870_05575"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX9251689.1"
                     /translation="MKISVLGAGGWGTTLGILLHYNGHQVTLWEYKKNYVKELIKKRI
                     NSIYLPGIPIPKEILITHDLKESVHQKNLLVLAVPSQFLRSVVDEISYSEIKNSILVS
                     VAKGIENKHLMTMSQMLKDVHPHLSDDQIGALSGPSHAEEVAKRIPTAVVAASTEHET
                     SKSIQAAFMNSYFRVYSSTDILGVELGGAFKNVIAIGAGIIDGAGFGDNTKAAIMTRG
                     VAEISRLGLAMGARPETFAGLSGMGDLIVTCMSKHSRNRYVGEQIGKGKKLKEVLKSM
                     DMVAEGVETSRSASRLAEKNNVETPITTEVYKILFEDKDPVKATTDLMTRDMKTERH"
     gene            complement(10140..10802)
                     /gene="plsY"
                     /locus_tag="VF870_05580"
     CDS             complement(10140..10802)
                     /gene="plsY"
                     /locus_tag="VF870_05580"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX9251690.1"
                     /translation="MFLLATIIILSYLVGSIPTSIIISKLSHGIDIREHGSGNAGGTN
                     VMRVLGWKKGLLVIILDALKGAFAVVVIARLHYGGLPFQNVSPFDDFTLVQIIAGIAA
                     VIGHIWTVFAGFRGGKGIATALGMLIMIITVDMLVAVGIFIIVVTVSRYVSLGSILAA
                     LSIPLTLIFRENILHDHIQSYGTLLPFVAAVSLLVVYTHRKNVMRLINGTESKISFKK
                     KK"
     gene            complement(10948..12432)
                     /locus_tag="VF870_05585"
     CDS             complement(10948..12432)
                     /locus_tag="VF870_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9251691.1"
                     /translation="MASVEGTKNYLSNFPEIKSKVLGKTGFEVSVCGFGSYRIDDGIP
                     NHQKALEKALLSGINILDTSSNYSDGGSEILIGKVLNKLISENKIAKENIVVVTKGGY
                     LQGSNLKLGIEREKSGKAFKDVVKCSPDLWHCISPDFLENQITLSLQRLNLDKIDVYL
                     LHNPEYFLTYSIISDPERREREYYRRIKDAFIHLEEEVKRGRISYYGISSNTFAEKET
                     KQNFTSLEKVIETANEITKQNHFAVVQFPMNLIEDGGMNNLNQQNQTKTFLQYAGENN
                     LGVLVNRPLNAISKNKLVRLADYPVTEDRNDNEIFSLIDDLNRQESNLIQKYINYMGL
                     SASEKKNVTDCISISSLLKSNYRKFDNPGNFNELKSTYLIPRANFAINEIGKSYPEDE
                     NIVRALRNYAVTTNILLDSIFSNLAKNKNLENEMFHSAINKFTNSEQKELSLSQKTIL
                     LINSVPQVTSTLVGMKSTNYVDDVQKSIKSEYIEKYADYWHNNG"
     gene            12584..12748
                     /locus_tag="VF870_05590"
     CDS             12584..12748
                     /locus_tag="VF870_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251692.1"
                     /translation="MAKVQSFADKAKGKKKVEGVTVKVIKSMKTDGGAYKFNEKFVKL
                     ADISKVTDIK"
     gene            complement(12767..13273)
                     /locus_tag="VF870_05595"
     CDS             complement(12767..13273)
                     /locus_tag="VF870_05595"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9251693.1"
                     /translation="MSETNFYSEETISGINIAVLVSSKGIKKIFLNPKKESQKFSTAT
                     KLRSDDPYLFGVFYQLNEYFAGTRKEFEVPLDIEGTDFQKKVWNELQKIPYGKTISYK
                     TLSEKLGDVKAIRAVGKANGQNPVAIIIPCHRVIGANGNLVGYAGGLDIKEKLLHLEG
                     ALNPELFD"
     gene            complement(13340..13882)
                     /locus_tag="VF870_05600"
     CDS             complement(13340..13882)
                     /locus_tag="VF870_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX9251694.1"
                     /translation="MLMVEDVDLTPNPHALKFILNEKLLNLETRQYADKESAENDPFA
                     KGIFEIDGVVSVFYMDKFVTIEKSPENNWGQIQRPFINFLKSFDKSLIPAEKEITVSD
                     EESSELLKQINNLLDQKVRPALAGDGGGLEVLGLDGYTVKIRYQGACGSCPSSISGTL
                     TAIEGLLKRDVNPSINVIPA"
BASE COUNT         4440 a         2556 c         2288 g         4768 t
ORIGIN      
        1 tgaaaaagaa aaactttatg caataaatga tattgtaatt gataaaggcg gctggcctaa
       61 aatgattgaa cttaccgttt gggtagatgg tgagtacgtt acaactttct cagccgatgg
      121 tttaattgtt gcaactccaa ccggatcaac tgggtattca atttcagttg gcgggccaat
      181 cgtttcacca aagacagatg tgattacgct ttcaccaata tcaccgcaca gtttaactgt
      241 tcgtccgttg gtgctgccta gctcgcaaga gatagtaatt aaagctgatt caccgcacaa
      301 agaaatccag gttagctgcg atggacaaag agtttttgat tttcctcctc ctacagaaat
      361 taaaatttgt aaaagcgatc gtccgttaaa attagttcac acatccttaa caacctattt
      421 tgaaacgcta agaaacaaat tgttgtgggg aattgattta agaataaaaa aagattagtg
      481 gctttttttc gtcattgcga acgatctcga ttcatcggga gagtgaagca atccaacaaa
      541 taataaaatt gagattactt cattcctcgc aatgaccatt ttagattttg acaaaaataa
      601 taattgtatc tgtcatttcg accaaaggga gaaatctgaa tgatatattt taccggattt
      661 ctcagtcgaa gacttcttcg aaatgacttt taataaatta aagacaagga ataagcgtca
      721 tagcaataaa ccgatgaaga aacatatgct caaatatctc atcccattag caataataat
      781 aaccgcaatc caaatatatt cacaggatta taaaatcatt cctgatgaaa aaaacggcga
      841 gccacttatc ttaggttact gtcctgcaag tgcaatgaat gattctgttt ttgatggcac
      901 ctggtctaca gaatacaaca actaccaacc ggattttgaa actctggata gcttagaagg
      961 aaaacttgaa gatgtaacta tcaaaattgt tttccgttct acctgcagcg atagcaggga
     1021 acaattgcca agattattta agatattaaa cgaacttaac tatgatgtta atacaattac
     1081 tcttattgga gttaacaggg aaaagcaagg tctatccaac gaaacagaag gacttgaaat
     1141 agaatttgtc ccgacgataa tcttttacaa aaatggaagt gaaattggaa gaatcattga
     1201 aacaccagta gaaagtttgg aaaaaggatt tgttgaagat cgttattgaa taaaaataaa
     1261 tcattgcaaa aaaaaccccg attcgcatcg gggttttcat tttaattctc tatctcagat
     1321 agttcaggaa gtttaatctt cttcggctta atatcatcct tctttccttt gccattgccg
     1381 tttccattac ttccgttctt accgttactc ttactcttgc ttccaaaccc ttcgggatta
     1441 tttgcatcag cataattttt atatgtgtat atcttatgct gaaagtttct gagaattatt
     1501 ctttcttcat ctttatgaag cacgtagttt ggtataatcg gaattttacc gccgccgcct
     1561 ggtgcatcaa ttacaaaatg aggaattgca agtccgcttg tatgtccgcg taaaccttca
     1621 agtatcttta ttcctgtttc gattgaagtc ctgaaatgat tagcaccttt ggtttcatca
     1681 gccatgtaca tatagtaagg acgaactcta atcttaagaa gtttctgcat caattctttt
     1741 acaactgcag gatcatcatt aacgcctttc ataataacca tctggttacc cattggtatt
     1801 ccggcatcag ccatcatctg gcaggcttta gaactttccg gtgtaatttc ccacggatga
     1861 ttaaagtgtg tgttgcagta aatcgggtga tacttcttta acatattaac aagctttgtt
     1921 gttatacgat gaggtagtac gcacggcatt ttagatccaa gacgaattat ttctatatga
     1981 ggaattgctc ttattctttg aagaattttt tcaagcattg catccgttaa cattaaagga
     2041 tcgccgccgg aaagaatcac atcacggatt tccgtatgct cttcaagata tttgaaagcg
     2101 ctttcaagtc ctttcatcga tatcttttca taatcgccga cttttctctt tcttgtacag
     2161 aagcggcaat acaatccgca ttggcttgta acgagaaaca gtgcgcgatc tggatatcta
     2221 tgagtaatgt ttggcacagg actcattgca tcttccatca aaggatcttc tgctgcatca
     2281 aaatcttcca gctcggcttt atccggaaca cattgacgcc agataggatc tccgggatgg
     2341 cgaattaagc ttaaataata tggattgata cgtgcctgaa aaaattcatc aagttcttcc
     2401 gcaactttgc ggtcgatccc gaatttttca acaagctgat caacagtgtg cacactgtct
     2461 cgtaccattt gctgccaaag ttccataacg tttcctcagt gattcttatt gttgaaatac
     2521 tttccaaata ccagcaggtc gtcacctact gagtagaaat ctcttatctc tgaaataatt
     2581 gagtatttat ttctaagata aaagttttgc gtggaagcat aactttcttt ggaagaagtt
     2641 tctgccagca gccatctgcc gttattactt cttacaaact cttcagcatg ttctaaaagc
     2701 tgcttgccga ttcctttgcc tgaggtagca ggatcggaag caatccaata gagatcataa
     2761 acaccatccg ttagcggccg cttaccagta caatgatatc caacaatttt gccatcattt
     2821 tcataaacat ataaatgata atctgtctga tcaggattca taactgaaat attcacaagt
     2881 tccattgcaa ctttaatttc ttcttcatta aagttaggaa tcctcgtcag catattttca
     2941 atagcaactt cgtcagacgg ctttattctt cgaatcattg tactttcgct gtaatgcaaa
     3001 actcgttatg gcaaataaaa gttcactata agattttcct gatgctgctg ctgctcttgc
     3061 gaaaccggaa tcacttgaca catcaggatt tggatttatt tcaattacgt aaggtattcc
     3121 atcgttagac aatctaacat caacccgggc ataatctctg caccccaaag ctttgtaagc
     3181 tttttttgca agttcttcaa ctttcttctg aatatcggaa tcgatcttag ccgggcagac
     3241 aggttttgta aaattataat atgtactgcc ttcaatccat ttgccgtcat aagtaacaat
     3301 cttaggcaag ccatctggta atcctgtaaa atctatttca gaaataggaa gaggtttatc
     3361 cccaagcaca gcacaattta attctctacc aactatgtat tcttcaacaa taagactttt
     3421 attataagtc tctgttaaaa aagtaaaatg tttttttaat tcgcggtagc tatttacaac
     3481 agaattttct gaaataccaa tgcttgcatc ttcatcaaga agttttaaga taagaggata
     3541 agtaagattg acatctttct ttgtaaattt ttgttttggt gtaagtgtaa ttgctctagg
     3601 agtattaatt ccgtttgaat ataaaatttc tttagctttc ttcttatcca ggcagttgcc
     3661 aagtgtttga ggaacgtttc cggtgtattg atagccaagc agttcatata atccggcgat
     3721 acaatattcg tagtaagtga ttccttcaac agactcaaca aagtttaata tcgcatcggg
     3781 attgtaagat gtaatttctg caatcgttct ggttacattt ttatctatcg gtaaagattt
     3841 tacatcagta aaaaatgttt taagatgatt ttcaatctct accatgttag aagcaaatga
     3901 atgttctgat aaatctttgc tgtccgaatt ttcttcagcg ggttttccat tataaatcga
     3961 aaacacactt acaggagaat tataacagat taatatttta gcttcggttt tcataaaaga
     4021 ttatatctct taattgctga tgctaaaact gtgtttatca tatcattgta ttcaattccg
     4081 gctgctcttg ccgctttagg gaagcttgaa ttctcatttg gatcaggaat aattccgggt
     4141 aaaggattaa tctctataat atttggttca ccatttttat caagccgcag atcaatcctg
     4201 ctccagtctc tgcactttag aactctgtaa gtatcagtac aaactttatt aatcttttgt
     4261 tctaactctt tagtgatatt tgcaggacaa tcaaaaacat caaactcgtt ttcttttgta
     4321 tcaaaaatcc acttagcttc ataggaatat aacggtgctg ttccttcagg atatttatca
     4381 tatcgcatct caattgccgg aagaatcttt aaatcaccat tattccccag catagccact
     4441 gtaaattctc tgccggataa aaattcttct atcaatgcag attgattata ctctgatgtt
     4501 attcttttta tttctctttt aagttcttct ttgttgtgta cgaaagatga agcataaatt
     4561 cctttaccgg aaccttcgct gatcggtttt acaattagag gaaagtcaag attatgattc
     4621 tctgcatcat caattctatc tgcaacaaaa aatttagcgg ttggaacttt gtaataactt
     4681 aaaatttctt ttgtgcgggc tttgtctaaa caaattgcaa gtgtaagagc gtcagaacct
     4741 gtgtaaggaa tttgtaacat atcaagcatt gcaggaattt gtgcctcgcg gcttacacca
     4801 aatgcacctt cagcaatgtt gaatacgata tcaggcttaa cttggcggaa tttattatac
     4861 gcttcttcgt cagcttcgat caaagtaaca ttattataaa gagagattgc atctttaaca
     4921 gcattaatag tttcccaggt atcccactct gcataagtat ccatttgcgc ttgcaaactt
     4981 tgtgaatgat tattaacaat tggagacact gatagggaga gagattcact ttctggtttt
     5041 aaattgtatg ctaatgcaac attcaacttc ttgttaaaga aggaagataa aatatttttc
     5101 tccttaataa tttttactgt tccaaatata atgtttataa aatgtttgtc aataagtttt
     5161 gaatttgtca ggaaaaatat ttttttgcgt tcatcaattt gcttttctga ttttaaaaac
     5221 ttttttttaa aatattttta agattcttaa ttcagtcaca tcaattgtaa tagctgttta
     5281 taatatcgaa acatagtcgg agtaaaaata ggttatgcga tcttagcgta agaaaggttt
     5341 tcggaataat gtttgataag atttttacgg atgagtgctg aaaaattttt ttctaatttc
     5401 ggatcaactt ctattaattt gaacgcactt tgcttcgctt caaggattaa ttcattgtct
     5461 tcaacaatgt ttgtgtactt taaatccgga aagccgcttt gcttagttcc gaaaatatct
     5521 ccgggtccgc gaagctttaa atctatctct gcaattttaa atccgtctaa atattttacc
     5581 atcgattgta atcttacaga tgatttatac ttttctaact gaacggaaga tagataatca
     5641 agctcaagtt cattcgcatt ttgcttggca gcaatttcat ctttggtaat taagatgcag
     5701 taagcttgtt tgctgcttct gccaactctt ccgcgcagtt gatgaagctg cgataaccca
     5761 aaacgatgtg cgtcattaat taaaattatg tttgcatcag gaatatcaat cccaacctcg
     5821 atcaccgttg tggaaaccaa aacatcaaac atctttttct gaaacatcag catcacttct
     5881 tctttttcct gccagttcat tcggccatga atcaatccaa gttttagatt tttaagatga
     5941 ttttctttta aatcttcata aaaagtaatt gctgctttca gatcaagttt ttcagaatcc
     6001 tcgactaggg gataaactaa aaatgtttga taaccttctt tggctttatc aataataaaa
     6061 ttataaatct cagggagttt cttttctccg cgtaaaactg tttttatcgg gattctattc
     6121 ttcggcattt catttataac ggacagatcc agatctgcat aaaccgtcat tgaaagagtt
     6181 cttgggattg gtgtagcgct cattacaagt acatcgggcg ttttgccttt actttgcagc
     6241 aacgctcttt gctttacgcc aaagcgatgc tgttcatcaa ttacaatcaa cccaaggtta
     6301 ttaaacttaa cctgttcttc aaaaattgcg tgcgtaccga ttataatatc cgcttcctgc
     6361 aattcaatat cagaaagttt ttgatttcgc acagatttct tttgtccgcc aagcagcaaa
     6421 ctaactttaa tttcttttct tttggtaata gatgaaagtt tgctcatcat tacagaaata
     6481 ttttttgcat gttgatcagc taaaatttct gttggcgcca tcaacgcagt ttgaaatccg
     6541 ttatcaatag ctattaacat tgaaattaat gcaacaatag tttttccact tccaacatct
     6601 ccctgcaaaa gtctgttcat cggtttttca gcaagcatat ctatttttat ttcactcaaa
     6661 acactcaatt gggatttagt taattcaaat ggaagtgttt taataaaatc agaaacaaga
     6721 tttgttttga tctccatctt gtttcctaaa agctttgttt gataattatt ctttcttatg
     6781 gcaacaagaa gctcaatgta aaacatttct tcaaacttaa agcggcgttt tgcctgctca
     6841 aacaattctt tgctttctgg ataatgataa tttttaacag cagttattaa atcaatcagc
     6901 ttatgccgat caataatttt ttgtggaaga gtttcctcaa gattatcaac ataactttca
     6961 acagctgtat taataattcg gcgtaaactt aaatcgccaa tatttttttg tttaagctct
     7021 ttgggaattc tatagaaagg aataatcttt ccagtgttga gaaaactttg tgattcctct
     7081 tcagtaatct tgtcaaaatc ggggtgaaca atttgaagtt tgttataatt tatttccggt
     7141 ttaccggaga ttgcaaaata atctccttca ttaaaaactg aatgaaaata ttttactcca
     7201 tggaaccaaa cacattcaat aaatccagac gagtctctaa actgaacttt aagaatttct
     7261 tttctgccaa gatttctttt ttctttatca gcaacttttc caatgatagt cacctcacca
     7321 tcataaccat tcattgcata tccataagcc ttagccgcat ttaaaactgt cgtacgatca
     7381 agatggcggg aagggaagta gaatatcata tcacgaattg tgttaatgcc aattttagaa
     7441 aatgattccg cacgtttcgg tccaacagat ttaatgtatt gagcagaaga ctcaagaata
     7501 ttttctttat cagatttcat tgcccgaaaa atagaggctt agcaaggcaa actcaacttt
     7561 acttataatt attaagcgat atttttttaa ctttccacac aacgaaataa aggaatttac
     7621 tttggattgg aaattactct taacagatga ccgtcttggg attgaaaaaa gtcattcagc
     7681 aaaaaaagat ggaagaagtc agtttcaaaa agattttgat cgaatagttt tttctccagc
     7741 attcagaaga ttgcaggata agactcaggt ttttccttta cccgaaagtg atttcgttca
     7801 tacaaggtta actcacagtc ttgaagtttc tgttgttgga agatcactcg ggaatctagt
     7861 tggagaaaga ataattgcca aacatccaaa actgaaaaaa gattttacac aattccattt
     7921 tggagaaatt gttgcagccg cttgtcttgc tcacgatatt ggtaatccgc catttgggca
     7981 ttccggtgaa gatgcaattg ctgagtattt taaaaacgga aacggtaaaa agttcaaaga
     8041 agaaattgtt gatgaaaaaa aatggaatga tttaattcgt tttgaaggta atgcacaggg
     8101 atttagaata attacaaagt tacaaaatca agatgttgat ggtggacttc aattaactta
     8161 tgctacttta tctgcattaa ataaatatcc caaagaatct ctaacagatc ttgagatcaa
     8221 acacgctaca ggacataaaa acatttacaa gaagtttgga ttctttcaat cagaaaaaga
     8281 aatatataaa catgttgccg agaaaacagg attgaccaaa cctgttactt ctgaaaaata
     8341 cttatggcat cgtcatcctc tttcttttct tgtagaagcg gcggatgata tttgttacag
     8401 tattatggat cttgaagatg gatttcgtct aggactttta acttttaaag agactgaaaa
     8461 cttattgctt ccgcttattc acaaacggga tttatctggt tataaaaaac gtgatgagaa
     8521 cgaaaaagtt ggttaccttc gtgcaaaagc tatcagcgat cttgttaatc ttgttgcaga
     8581 tgcttttatg gaaaatgaaa aagatattct tgaagggaac tttaataaag aactcgtaac
     8641 tgtaatttca aaatcaaata ctctcatgaa aattgagaaa gcttctattg caaaaattta
     8701 cagacacaga agtgttgttg agcgtgaggt tgccggttat gaagttctag gtggtttgct
     8761 tgatactttt attaccgcgt acaatgaacg ttctagcgat aatcttacac ccaaaaatcg
     8821 tgcaatcata aatcttattc ccaagaggtt tttctttgca gaaaaaaata atccggaact
     8881 ttatcaaaga cttttaagag ttatagattt tatttccgga atgacagact catatgccgt
     8941 ttctatcttt agaaaaatta gaggaatatc tttgccgggt ggaagagtaa cagaatagtg
     9001 ctatgttgtc attgcgaacg agccttcgag tgaagcaatc tattttataa gcggaaaagt
     9061 aattcatatt aggtgataag ttgtaaggca gattgcttca ccgattaaag tgggaatcgc
     9121 aatgacaaag aaaatgtgtt aatgtctctc agttttcata tcgcgggtca taagatcagt
     9181 tgttgcttta accggatctt tatcttcgaa cagaatttta taaacttcag tagtaattgg
     9241 agtctctaca ttattttttt ctgcaagcct ggaagctgaa cgactcgtct caacaccttc
     9301 agcaaccata tccatgctct ttaaaacttc tttaagtttt ttaccttttc caatttgttc
     9361 accaacataa cgattacgac tgtgcttgct catacacgta acaatcaaat cacccatacc
     9421 ggaaagacct gcaaacgttt cgggtcttgc acccattgcc aaaccaagcc gggaaatttc
     9481 tgcaacacca cgagtcataa tagccgcttt tgtattatcg ccaaaacccg caccatcaat
     9541 aattcccgca ccgatagcga taacattttt aaacgctccg ccaagttcaa caccaagaat
     9601 atcagttgaa gaataaacac ggaagtaaga attcataaat gcagcttgaa ttgattttga
     9661 agtttcatgc tctgttgatg cagcaacaac agctgtggga attctttttg caacttcttc
     9721 tgcatgcgat ggacctgaca aagctccgat ttggtcatca cttaaatgtg gatgaacatc
     9781 ttttaacatt tgactcattg tcatcaaatg tttgttctca attccttttg caacgctaac
     9841 aagaatcgaa ttttttattt cagagtagct tatctcatca actacggagc gcagaaactg
     9901 tgatggaacc gcaagcacca gcaagttttt ttgatgaact gattctttaa gatcgtgagt
     9961 gattaatatt tctttaggaa ttggaatgcc gggcaggtaa atggagttaa ttcttttttt
    10021 gattaactct tttacataat tttttttgta ttcccacagg gttacctggt gaccgttgta
    10081 atggagaaga ataccaagtg tagttcccca accaccagca ccgagtactg aaattttcat
    10141 tatttctttt tcttaaagct tattttactt tctgttccgt taatcaatcg cataacgttt
    10201 ttgcgatgag tgtaaactac aagtaaagaa actgcggcaa caaatggaag caatgttcca
    10261 taactttgaa tatgatcgtg aagtatattt tctctgaata tcagagttaa tggaattgac
    10321 agtgcggcta atattgaacc aagagagaca taacgagaaa ccgtaactac aattataaaa
    10381 attccaactg ctacaagcat atcaacagta ataatcatta ttaacatacc gagtgctgtg
    10441 gcaattcctt ttccaccacg gaagcctgca aacacagtcc agatatgacc tattacagct
    10501 gcaataccag caatgatttg aactaatgta aaatcatcaa acggagaaac gttttgaaat
    10561 ggtaatccgc cataatgcag tcttgctata acgacaacag caaacgcacc tttaagtgca
    10621 tctagaataa ttacaagcaa accttttttc cagccaagta cacgcataac atttgtgccg
    10681 ccggcatttc cgctgccgtg ttcacgaata tctatgccat gagagagttt acttattatt
    10741 atactcgttg gtattgaacc aactaagtaa gaaagaatta taattgttgc cagtaaaaac
    10801 attaaaaatc caaaaaattg ttataaaacc taatcattat ttaataaaga atcaacgata
    10861 agaaatattt gataaatgaa aaattatttt tcaaacataa gttagactta atttgttctt
    10921 aaaagattca tttaattttt ataaaattta accattatta tgccagtaat cagcatattt
    10981 ttctatatac tcacttttta tcgatttttg gacatcatct acataatttg ttgatttcat
    11041 tccaactaaa gttgaggtta cctgaggcac actattaatt aatagtatcg ttttttgaga
    11101 tagcgataat tctttctgtt cactattggt aaatttattt attgccgaat ggaacatctc
    11161 attttcaagg tttttgtttt tagccagatt actaaatatt gaatccaata aaatatttgt
    11221 tgttacagcg tagtttctta aagcccgaac aatattttca tcttcaggat aacttttacc
    11281 tatttcattt atagcaaagt ttgcacgcgg aattaaatat gtacttttaa gttcattaaa
    11341 attgccagga ttatcaaact ttctatagtt tgattttaaa agtgatgaaa tggaaataca
    11401 atcagtgacg ttttttttct cacttgcact caaacccata taattaatgt acttttgtat
    11461 caagttggat tcttgtctgt tcaaatcatc gattaggcta aatatttcat tatcgtttct
    11521 atcttctgta acaggataat cagccaatct tacaagtttg tttttactta ttgcattaag
    11581 cgggcgattt accaaaactc caagattatt ttccccggca tattgcaaaa aagttttagt
    11641 ttgattctgc tgatttaaat tattcattcc accatcttca ataagattca tcgggaattg
    11701 aacgactgca aaatgatttt gtttagttat ttcatttgct gtctcaataa ctttttccag
    11761 cgatgtaaaa ttttgttttg tctccttttc agcaaaagta ttcgatgaaa ttccgtagta
    11821 agaaattcta cctcgcttaa cttcttcttc caggtggatg aacgcatcct taattcttct
    11881 atagtactcc cgctcccttc tttcagggtc agaaatgatg gagtaggtta aaaagtattc
    11941 cggattatga agaagataaa catcaatctt atccaaattt aatctttgca aagaaagtgt
    12001 aatctggttt tcgagaaaat ctggacttat gcaatgccac aaatctggac tgcatttaac
    12061 cacatcttta aatgctttgc cagatttttc tctttcaatt ccgagtttaa gatttgatcc
    12121 ctgaagatat ccacccttag taacaacaac tatgttttct ttagcaattt tattttccga
    12181 gataagttta tttaaaactt taccgatgag aatctcactg ccgccatccg aataatttga
    12241 tgaagtatca agaatattta ttcctgaaag taaagctttc tctagagctt tttggtgatt
    12301 tggaatccca tcatcaattc tataactgcc aaatccgcaa acagaaactt caaatccggt
    12361 tttacctaaa actttggatt tgatttctgg aaaattggaa agataatttt ttgttccttc
    12421 tactgaagcc ataaatcccg taaaatcata aaattgtgct gcaaaagtat aaaaaatgga
    12481 cggattctca aggttttaat ttacaaaatg taacttaaac taaatctttt ctttaaaacc
    12541 ttaatgctat atatttacac ctcaaaattt taggattaaa aagatggcaa aagttcagag
    12601 ttttgcagat aaagcaaaag gcaagaaaaa agttgaaggc gtaactgtaa aggttattaa
    12661 aagtatgaaa accgatggcg gcgcttataa attcaatgaa aagtttgtta agcttgctga
    12721 tattagcaaa gtaacagata ttaaataatt ttagcgtttg attttttcaa tcaaacaatt
    12781 caggatttaa tgctccttct aaatgaagga gtttttcttt tatatccaac ccaccggcgt
    12841 aaccaaccag atttccgttt gctccaatta cacgatgaca tggaattatt atagcaacag
    12901 gattttgccc gtttgctttt ccaacagcac gaattgcttt tacatcacca agtttttccg
    12961 ataatgtttt gtagctgata gtttttccgt aagggatctt ttgcaattca ttccaaactt
    13021 ttttctgaaa gtccgttccc tcaatatcta atggaacttc aaattctttt cttgtaccgg
    13081 caaagtattc attaagttga taaaaaactc cgaacaggta tggatcatca cttctaagtt
    13141 ttgtagcggt tgaaaatttt tgggattctt ttttaggatt caggaatatt tttttaatcc
    13201 cttttgatga aacaagaaca gctatgttaa taccggaaat agtttcctcg gaataaaaat
    13261 ttgtttcact cataatctgt cctgataaat aaaaaaggct gctaaattca gcagcccttt
    13321 aagaatcaaa taaaaatatt taagccggta ttacatttat tgaaggatta acatcacgtt
    13381 ttaataaacc ttcaattgca gttaatgttc cgcttattga acttgggcag cttccgcaag
    13441 ccccttgata acgtatctta actgtgtagc catctaaacc taaaacttcc aaaccgccac
    13501 catctcctgc aagtgccggt ctaactttct gatcaagtaa attgtttatc tgttttagca
    13561 gctcgcttga ttcttcgtcg ctaactgtaa tttctttctc agcgggaatt aaactcttgt
    13621 caaagctctt taaaaagtta ataaacggtc tttggatttg tccccaatta ttctcggggg
    13681 atttttctat tgttacgaat ttatccatat agaaaacaga tacaacacca tcaatttcaa
    13741 aaattccttt tgcaaatgga tcattctctg cagattcctt atcagcatat tgtcttgttt
    13801 ccagatttaa taatttttcg ttcaaaataa attttagtgc atgaggattt ggagtcaaat
    13861 caacatcttc taccattaac ataattacac ctttcttgtt tatacttgta cgaaaaataa
    13921 actttcataa tttactctgc aagtcagtaa aaacttatga ttataattcg aatagatttc
    13981 tttttagaat tgaagttaaa agctattttt ggtcacaaaa atttgagaaa taaatgaaaa
    14041 aaatattagt tg
//
LOCUS       DASWBY010000299         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_273369, whole genome shotgun sequence.
ACCESSION   DASWBY010000299 DASWBY010000000
VERSION     DASWBY010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273369"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VF870_05605"
     CDS             <1..602
                     /locus_tag="VF870_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX9251695.1"
                     /translation="NRIRYDFELLQPAMQFVIDLGEKLTELSPNINAIPKIDKSIFRL
                     YRDVRFSKDKVPFKTNLGLYFWEGRGKKMECSGLYFHIEPKLFFLGAGMYVFTKDQLK
                     KYREIVSDPVKGKQLNDVLSKLLKNKKFELGGKTYKKTPRGFDPDYIYNDLLLHSGLY
                     VYYESTDFNELTKTDPVIFAYKIFKELFPVHKWFVENLI"
     gene            771..2099
                     /locus_tag="VF870_05610"
     CDS             771..2099
                     /locus_tag="VF870_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEX9251696.1"
                     /translation="MKYNSYMLHNFKNIAQIQSISNELIDAIEIVGSVLPFKTNNYVV
                     DNLIDWNNIPDDPMFKLTFPQKGMLIPEHYQRMKSVIHSGADKQTIKEVANEIRLELN
                     PHPAGQLEHNVPMIEGEKLFGMQHKYRETVLFFPSQGQTCHAYCTFCFRWPQFVGMED
                     LKFASKEADLLVKYVKAHTEVTDVLFTGGDPLIMKTKHLEAYIQPLLEANIPHLRNIR
                     IGTKALGYWPHRFLTDDDAEDLLILFEDVKRARKHLALMAHFNHPVELTGDAIAEAIR
                     RILKTGSVIRTQSPILKHINDSAENWIDMLKKQVALGCIPYYMFIARNTGAQHYFSIP
                     LIEAWNIFREAYQSVSGICRTLRGPSMSCLPGKVQILGVADVKDEKVMTFRMIQGRNP
                     TWAARPFFAKYDEYAIWYTDLRPAFEEEKFFFTDELNEILSPAEIECDFE"
     gene            2092..>3265
                     /locus_tag="VF870_05615"
     CDS             2092..>3265
                     /locus_tag="VF870_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9251697.1"
                     /translation="MSKNNNTDKNQFDISLNLNVRGAPLSATLLINEISNKLISEGRK
                     VYKLGLGQSPFPVPQMVVDELKVNAHQKDYLPVKGLKQLRQAVADYHSRRNDIRRNAE
                     NVLIGPGSKELMFLLQFVYYGDLVIPTPSWVSYAPQAKFIGRNVDWLNTTEKNKWRIA
                     PQDLENLCKRDPDKPRIVILNYPSNPTGATYSVSELKEIAAVAKKHHIVLLSDEIYGE
                     LHFEGKHISIAKFYPEGTIISSGLSKWCGAGGWRLGTFTFPSSLNWLLEAMSSMASET
                     FTSTSAPIQYAAVCAFRGGISIERYLNNTRRVLKSLGLSIHKKLDKAGISAPKPEGGF
                     YLFCNFNKHADRLKKVGITDSSQLVQKLIEDTGVALLPGSVFGRPENELTARLASVD"
BASE COUNT         1068 a          582 c          640 g          975 t
ORIGIN      
        1 aaaatcgaat tagatatgac tttgaacttc tgcagcctgc catgcagttt gtaattgatc
       61 ttggagagaa actaacagaa ctatctccta acattaatgc tattccaaaa atagataaat
      121 caatctttcg gctttatcgt gatgttcggt ttagcaaaga caaagttccg tttaaaacaa
      181 atcttggctt gtacttttgg gaagggcgag gaaagaaaat ggaatgttcc ggcctttatt
      241 ttcatatcga accaaaactg tttttccttg gtgcaggaat gtatgtgttt actaaagatc
      301 aactaaaaaa gtatcgtgaa attgtttctg atcctgtaaa aggcaaacag ctaaatgatg
      361 ttctttcaaa gcttctaaaa aacaagaagt ttgagcttgg tggaaaaact tataaaaaaa
      421 ctccgcgcgg atttgaccct gactacatat acaatgattt gcttttgcac agtggattat
      481 acgtttatta cgaaagcact gattttaatg aattaactaa aacagacccc gttatttttg
      541 cttataaaat atttaaagaa ctctttcctg ttcataagtg gtttgtagaa aacctgattt
      601 agacttgctt ttctcataaa aaaccatatc ttgcattaaa aatcatatct ttaattaggc
      661 tgtttcgtaa ttctatttat tattaatgct atcaaatgag cttgatggca aataattatt
      721 tcagtcagaa aaatctacaa acaacctagt aatctgaaag gagatgagct atgaagtata
      781 attcatatat gctgcataac ttcaaaaaca tcgcccaaat acaatctatt tctaatgaat
      841 taatcgatgc aatagaaatt gtgggatctg ttcttccatt taaaacaaac aattatgttg
      901 tggataatct tatagattgg aataacattc ccgatgatcc tatgttcaaa cttacttttc
      961 cgcaaaaggg tatgctgatt cccgaacatt atcaaaggat gaaatctgtt attcatagcg
     1021 gcgcagataa acaaacaata aaagaagtgg caaacgaaat aagacttgaa ctaaatcccc
     1081 atcccgctgg acaattagaa cataatgtcc ctatgattga gggcgagaaa ctatttggaa
     1141 tgcaacataa ataccgtgag accgttttat tctttcccag ccagggacaa acctgtcatg
     1201 cttattgcac attctgcttc cgctggcctc agtttgttgg aatggaagat ttgaagtttg
     1261 catcaaaaga ggctgatctg cttgttaaat atgttaaagc acataccgaa gtaacagatg
     1321 ttctttttac gggtggtgat ccgttaataa tgaaaaccaa gcatcttgaa gcttacatcc
     1381 agccattgtt agaggctaat atcccgcacc ttagaaatat aagaatcgga actaaggcgc
     1441 ttggttactg gccccatcgg tttctaaccg atgatgatgc tgaggatctg cttattttgt
     1501 ttgaagatgt aaaacgcgca agaaaacatc ttgcactaat ggctcacttc aatcacccgg
     1561 ttgagttaac tggtgatgct attgctgagg caattagaag aattttaaaa accggatcag
     1621 taattagaac ccaatcacct attttaaaac acataaatga ttcagctgaa aattggatag
     1681 atatgttaaa gaagcaagtt gcacttggct gtatacccta ctacatgttt attgccagga
     1741 ataccggtgc tcaacattat ttttctattc cgcttattga agcatggaac atttttagag
     1801 aagcctatca atctgttagc ggtatttgca gaacactgcg cggaccaagc atgtcttgtc
     1861 tgcccgggaa ggttcaaata ctcggtgttg cggatgttaa agacgaaaaa gtgatgacgt
     1921 ttagaatgat tcagggaaga aatcctacgt gggctgcacg cccgtttttt gcaaagtatg
     1981 atgaatatgc tatttggtac actgatctaa gaccggcatt tgaagaagaa aaatttttct
     2041 ttacagatga attaaatgaa atattatctc ctgcagaaat agagtgtgat tttgagtaaa
     2101 aacaacaata ccgataaaaa ccaatttgac atcagtttaa acctgaatgt cagaggcgca
     2161 ccactttctg caactttgct gataaatgaa atttctaaca aattaatcag cgaaggaaga
     2221 aaggtatata aacttggatt ggggcagtca ccgttcccgg tgccgcaaat ggtggttgat
     2281 gaactaaaag taaatgcaca tcaaaaagat tatctacctg taaaaggatt aaaacaatta
     2341 agacaagctg ttgcagatta tcattcccga agaaatgaca tccgcagaaa tgccgaaaac
     2401 gttctcatcg gacctggttc aaaagaatta atgttccttc tgcagtttgt gtattacggt
     2461 gatctggtta ttccaacgcc aagctgggtt tcttacgcgc cgcaggcaaa gtttattgga
     2521 agaaatgttg attggctgaa cacgactgaa aaaaataaat ggcggattgc accacaggat
     2581 ttagaaaatc tttgcaagcg cgatcccgat aaaccgagga ttgttatact taactatcct
     2641 tcaaatccga cgggggctac ttattctgta agtgaattaa aagaaatagc tgccgttgca
     2701 aaaaaacatc atatcgtttt gctttctgat gagatttacg gcgaacttca tttcgagggg
     2761 aagcatattt ctattgctaa gttttatccc gagggaacta taatatccag cggtttaagt
     2821 aagtggtgcg gtgccggtgg ctggcgtttg ggtacattta cttttccatc atcactaaat
     2881 tggctccttg aagcaatgtc atctatggca agtgaaacct ttacttcaac aagtgcaccc
     2941 attcagtatg ctgcagtatg tgcctttcgg ggtggaatta gtatcgaaag atatcttaac
     3001 aatacaagga gagttttaaa atccctgggt ttatcaatcc ataaaaaatt ggataaagcc
     3061 ggaattagtg caccaaaacc tgagggcgga ttttatttgt tttgtaactt taataaacat
     3121 gccgacagac ttaaaaaagt tggaattaca gatagttcac aacttgtgca aaaacttata
     3181 gaagataccg gcgttgcgtt gcttccgggc agcgtgtttg gaagaccaga gaatgaatta
     3241 acagctcgtc ttgcctctgt tgatt
//
LOCUS       DASWBY010000300         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_575621, whole genome shotgun sequence.
ACCESSION   DASWBY010000300 DASWBY010000000
VERSION     DASWBY010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575621"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VF870_05620"
     CDS             <1..958
                     /locus_tag="VF870_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9251698.1"
                     /translation="GNPGGLLDVAVDICDKFLKKDLLIVSTRGKELSSEKKYYALEEP
                     LVGKEKLIVLINGNSASASEIVAGAIQDHDRGVILGTQSFGKGLVQTITPLSFNTSLK
                     ITTAKYYTPSGRCIQRIDYSKDNQVIPEPDSLKQGEFSTDNKRVVYSAGGITPDTTVK
                     NGIESELLKDLLAKGIIFDFADKYYYQNVNSDFNKIKDDKILSEFKKYLIDSDYKYES
                     DVEKKINDIVKDLNEKHTANDLSKTLEKLDSEIKQIFDVEFAESTNEILSELKIELAH
                     RYFGNDAEIKEALKYDKQFNAALMLFNDDVTYSKLLSSRKLN"
     gene            1645..2217
                     /locus_tag="VF870_05625"
     CDS             1645..2217
                     /locus_tag="VF870_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9251699.1"
                     /translation="MKKSFLDYIFRNRLPVTLFVVVASFTLTFFISRAERDGVGYTPD
                     QPIAYSHKLHAGDMKIDCQYCHVGVEKSRHAMVPAVATCMNCHTVARKDRPEIIKLTE
                     YYRDGKPLRWKRIHKVPDYAYFNHSVHVNKGIDCTACHGQITQMEKVGQMNSFTMGSC
                     LNCHRNAHDKLPELKDKIKSGPEYCSACHR"
     gene            2250..2426
                     /locus_tag="VF870_05630"
     CDS             2250..2426
                     /locus_tag="VF870_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251700.1"
                     /translation="MNRKKFFTSISLGAVGFAVYNSFPMKFFSKPSAFNNNKIEVKVN
                     PLAVSRKKVGGKNV"
BASE COUNT          924 a          354 c          435 g          829 t
ORIGIN      
        1 agggaatcca ggcggattgt tggatgttgc tgtagatatt tgtgataaat ttttgaagaa
       61 ggatttatta attgtttcaa caagaggtaa agaactaagc agtgaaaaaa aatattatgc
      121 attagaagaa cccctggtcg ggaaagaaaa actaattgtt ttgataaatg gaaatagtgc
      181 ttccgcttcc gaaattgtag ccggcgctat acaggaccac gataggggag taatacttgg
      241 aacccaatct tttggtaaag gattagttca aaccataact ccattaagtt ttaacacttc
      301 gctcaaaata accaccgcaa aatattacac tcctagcgga agatgtatac agagaataga
      361 ttactcaaaa gataatcagg taattcctga accggattca ttgaagcaag gtgaattttc
      421 tacagataac aaaagagtag tttatagtgc cggtggaata acaccagata caactgtaaa
      481 aaatggaatc gaaagtgaat tattgaaaga tcttttagcg aagggtatta tctttgattt
      541 tgcagataag tattattatc aaaatgttaa ttcagatttc aataaaataa aagatgataa
      601 gattctatct gaatttaaaa agtaccttat tgattctgat tataaatatg aatcggatgt
      661 agaaaaaaaa ataaatgata ttgtaaaaga tcttaatgaa aaacataccg ctaatgattt
      721 aagcaagacc ctcgaaaaat tagactcaga aattaaacag atttttgatg ttgaatttgc
      781 tgaaagtact aatgagatat tatcagaatt aaaaattgaa cttgcacata gatattttgg
      841 aaatgatgct gaaataaaag aagcattaaa atatgacaaa cagtttaatg ctgctttaat
      901 gctttttaat gatgatgtaa cttattctaa gttattaagc agtagaaaac ttaattagtc
      961 ttttattata ttataatgtt ttatgattaa atcatttttg cgagaagcta aagcttttta
     1021 taggattaat ccgacatata atagctgtcc gtcgtgtaaa agttcgggta ctataatgcg
     1081 gtcgcacagc aggaatgtaa aagaaaaaat cataaacaaa atttcgttcc ataaatatta
     1141 tcgctgtaaa aattgtggtt ggcgtgggtt attagcaaca gtgagggtta cttccacatc
     1201 aatttctgta atgttccttt acctgttaat aatttttgtt gttggagtta ttacatatca
     1261 aattcttaaa agacttttat agccggggtt ttgaattgag catcttttga atttgataac
     1321 aaaaataaaa tattactgac taatcattaa caattttttt attatagctc attcaaaata
     1381 aaaaatattg aaaagtcttt aataaaatat cttataataa cttattcctg atattttaaa
     1441 gattcatttg atttaaaagc ataaatttat aattttaacc tttcgaaaaa gaaatagtta
     1501 gttatggtca aatagggaag tcctgctatt ttccctggtt aatccgtgca tatttaatct
     1561 aaataataat ataaatttta caggattttt cctgttttgt gtgtcttttt taatttttag
     1621 ccctaaataa ttaaagggat tctgatgaaa aaatcttttc tggattatat ttttagaaat
     1681 agattgcctg ttacattgtt tgtagttgtt gcatctttta cacttacatt ttttatctcc
     1741 agagctgaaa gagatggggt tggttacaca ccagatcaac caattgctta ttcgcataaa
     1801 ctccatgccg gagatatgaa aattgattgc caatactgcc acgttggtgt agaaaaatcc
     1861 agacacgcta tggtacctgc agtcgcaact tgtatgaatt gtcacacagt tgctcgtaaa
     1921 gacagacctg aaattattaa acttactgag tactatcggg atggtaaacc tttacgatgg
     1981 aaacggattc ataaagttcc ggattatgct tacttcaatc actcagtcca cgttaacaaa
     2041 ggaattgatt gtacagcttg ccatgggcaa attacccaaa tggaaaaagt aggacaaatg
     2101 aactctttta cgatgggaag ttgtttaaac tgtcatagaa atgctcacga taaattacct
     2161 gagctaaaag ataaaattaa atccggtcca gagtactgtt cggcatgtca cagataatga
     2221 tatttgaaaa tcaataaaat aaaattggta tgaatagaaa aaaatttttt acatcaatct
     2281 cattaggcgc agttggattt gcggtctata actcatttcc tatgaagttc ttttctaaac
     2341 cctcagcttt taacaacaat aaaattgaag ttaaagtaaa tcctcttgca gtaagcagaa
     2401 aaaaagtagg tggtaagaat gtctgagttt aaaactgacc aaacagattc attacaaaat
     2461 tcaactcctg atcctaatta ctggagaagt tttgaagcac tttatggaga tccggaagtt
     2521 gttgaagcta ataatcatga at
//
LOCUS       DASWBY010000301         4210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_423859, whole genome shotgun sequence.
ACCESSION   DASWBY010000301 DASWBY010000000
VERSION     DASWBY010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423859"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VF870_05635"
     CDS             <1..573
                     /locus_tag="VF870_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9251701.1"
                     /translation="VDSTIKYRPFLKGIRVAKQKEVLKKSLTEYDYYPNFNLALQYSF
                     RDKIESTNTPLDDFFSVMVGISLPLNYGGKISSKVEETETMQKLYDEQYNMSLQVLQS
                     SFGSSVAKLNSLQGRIKLVEEGSLIQAKENLKSTLTSYQVSQIDFLNVIDAQNSLLKI
                     ENDLYRLKTDYLNEIAELEFLTGTEFYIGE"
     gene            575..2287
                     /locus_tag="VF870_05640"
     CDS             575..2287
                     /locus_tag="VF870_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9251702.1"
                     /translation="MKKKIIIIVIAAVVILVPVYFLFITGGSSDITSGDKQLYTCGMH
                     PEIIRDKPGLCPICEMQLVPIKNKNQKSGEKKILYWRAPMDPNEIYDAPGKSKMGMDL
                     VPVYEDEAGASGIVTIDPEVQQNMNVKISRVEAKQLSAKVTTNGVLATNETSEYIVTT
                     RINGWVDKLYVNYTGQQIVKGAKLMDIYSPELVSAQQELLTALSYQNSVNASSLESIR
                     ESGNELVKNAIRKLELLEVSDAEIKRLINTKEVKTYITLYAQKSGTVLEKNIVEGQKV
                     MAGMPLLKIANLSTLWLMADIYEYELNKVKVGSKASISFNFYPDKNYVGKVSFIYPTI
                     DPKSRTVKIRIELNNSGELKPSMFANITIKGEELGKKPVVPENAVIRSGMKDIVIISL
                     GDGKFKPQEVKLGSYSDGYYQVLSGLTEGNAIVTSAQFLIDSESNLKAAINQFQTAGS
                     DTSSMKMNNMNKAVETPEKNEKTTMRMNEEQNNPSSIVHTGVIDVESIDKNKDGKLWE
                     CPMDWNVISDESGRCPLCNMKLKEYSIDEIKANLDKNGYEYKNNITTSLRGAERRSNL
                     LIII"
     gene            2356..>4210
                     /locus_tag="VF870_05645"
     CDS             2356..>4210
                     /locus_tag="VF870_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX9251703.1"
                     /translation="MITSDQNKDGLIAKLIEWSINNKFLVIIFTAALVGLGIWAVSTT
                     KVDAIPDLSDVQVIIITEYPGQGPRIVEDQVTYPLTTKMLSVPYAKDVRGYSMFGYSM
                     VYIIFEDGTDIYWARSRVLEYLNGLQGTLPAGVNPQLGPDATGLGWVYQYVLNSDKKD
                     LQELRSIQDYFLKYELSSIQGVAEVASIGGYVKQYQVNLDPIKLASYNIPLQKVKMAI
                     KQSNNDVGGRLLEMGEMEFMVRSLGYIKSKEDLEDVAIGVSPNSGSPIYLKDVADISI
                     GPELRRGLADWNGEGETVGGIIVIRYGENALAVIDRVKERLEELKKSLPADVTIDVAY
                     DRSGLINRSIDNLSNKLIEESLIVALVIIAFLLHVRSSFVAIFTLPTAVLISFLIMRL
                     QGINANIMSLGGIAIAIGAMVDASIVMVENAATHLSSENDKPPHERKSHWATILQSAK
                     EVGPSIFYSLVVITISFLPVFTLEQQEGRMFRPLAFTKTYAMAGAAILAVTIVPILIG
                     YFVRGRIRKEEDNPITKILVKIYHPIVDFVMKRRWWVMASALIIVLITILPFSKLGSE
                     FMPPLYEGDLLYMPTTLPGISITKARELLQQTDKIIKSFPEVESVFGKIGRA"
BASE COUNT         1436 a          689 c          858 g         1227 t
ORIGIN      
        1 gttgatagta ccataaagta tagaccattt ctgaaaggaa tcagagtagc aaaacagaaa
       61 gaagtactaa agaaatcttt aactgaatat gattactatc ccaattttaa tctcgcactt
      121 caatattctt tcagggataa aattgaaagt accaataccc cccttgatga tttcttttca
      181 gtgatggtcg gaatttcctt acctcttaat tacggtggta agatcagctc aaaagttgaa
      241 gaaactgaaa ccatgcagaa actttatgat gagcagtaca atatgtctct gcaagtgctg
      301 caaagctcat tcggttcatc agtagcaaaa cttaattcac ttcagggaag aattaaactc
      361 gttgaagaag gatcattaat tcaggctaag gaaaatctaa aatcaacttt aacaagctac
      421 caggttagcc aaattgattt ccttaatgtt attgatgcac agaatagttt attgaaaatt
      481 gaaaatgact tatatcgttt gaagacagat tatttaaatg aaattgcaga acttgagttc
      541 ctgacgggga cagaatttta tataggagaa taaagtgaaa aagaaaatta taataatagt
      601 aatcgcagca gttgtaattc ttgtgccggt ttatttttta tttataaccg gaggatcttc
      661 tgatattaca tctggggata aacagttgta tacctgtgga atgcatcccg aaataattag
      721 ggataaaccg ggcctttgcc cgatttgtga gatgcaattg gtgccgatca aaaataaaaa
      781 tcaaaaatcc ggtgaaaaga aaattctata ctggcgagca ccaatggatc cgaacgaaat
      841 ttatgatgct ccgggtaaat caaaaatggg aatggatctt gttcccgttt atgaagatga
      901 agccggtgca tcaggaatag taaccatcga tccggaagta cagcaaaata tgaatgttaa
      961 aatttccagg gttgaagcaa aacaactgtc agctaaagta acgactaatg gtgttttagc
     1021 aacaaatgaa actagtgaat acatagttac aacaagaata aatggatggg ttgacaaact
     1081 ttatgttaat tatacaggtc agcagattgt aaaaggtgct aaattaatgg atatatattc
     1141 gcctgaactt gtttctgctc aacaggaatt actaactgcg ctttcatatc agaattctgt
     1201 taatgcaagt tctctcgaat caattcgtga aagtggtaat gagttagtta agaatgctat
     1261 tcggaaactt gaactgcttg aagtttctga tgctgaaata aaaagattaa ttaatacaaa
     1321 agaagtcaaa acatatatca ctctttatgc tcaaaaatca gggacagtcc ttgaaaaaaa
     1381 tattgttgaa ggacagaagg ttatggcagg aatgccgtta cttaaaatcg caaacctgtc
     1441 tactctctgg ttaatggcag atatttatga atacgaacta aataaagtta aagttggatc
     1501 gaaagcttct ataagtttta atttttaccc cgacaaaaat tatgttggta aggtctcgtt
     1561 catttatcca actatcgatc ctaaatccag aacagtaaaa atcagaattg aactaaacaa
     1621 cagtggtgaa cttaagccat caatgtttgc aaacattact attaaaggcg aagagcttgg
     1681 caaaaagcct gttgtacctg aaaatgctgt tatccgtagt ggtatgaaag acattgttat
     1741 tatttctctc ggtgatggaa aattcaaacc gcaggaggtt aagctcggaa gttattcaga
     1801 tggatattat caggtgctta gcggattaac cgaagggaat gcgatagtta cttctgcaca
     1861 attcttaatt gattctgaaa gcaatcttaa agctgccata aatcagtttc aaactgctgg
     1921 atcagatact tcttcaatga aaatgaataa tatgaataaa gcagttgaaa cacctgagaa
     1981 gaatgaaaaa acaacaatga gaatgaacga agagcagaat aatccttctt ccatagttca
     2041 tacaggtgtg attgatgttg agtctattga taaaaataag gacggtaaac tttgggaatg
     2101 tccgatggat tggaatgtaa tttcagatga gtcaggaaga tgccctttgt gcaatatgaa
     2161 attgaaagaa tattcaattg atgaaataaa agctaatctg gataagaatg gttatgagta
     2221 taaaaataac ataacaacgt cattacgagg agcggagcga cgaagcaatc tgttaataat
     2281 tatttaagat tgcttcatcc cgatgaatcg ggattcgcaa tgacaatcgc aaaaaaagtt
     2341 tttcggagaa attaaatgat aacatcagat caaaataaag acggattgat tgcaaaactc
     2401 attgagtggt caatcaacaa taaatttttg gtaatcattt ttactgcagc cctcgttggt
     2461 ttgggaatct gggcagtatc aactacaaag gttgatgcga tccctgatct aagcgatgtg
     2521 caggtaatca tcatcacaga atatcccggg cagggaccac gtattgttga ggatcaggta
     2581 acatatccac ttactacaaa aatgctatca gttccctatg caaaagatgt tcgcggttac
     2641 tcgatgttcg gctactcaat ggtttacatc atctttgaag atggtactga tatctactgg
     2701 gcacgaagtc gggtgcttga atacctcaat ggattacaag ggacactgcc ggctggtgta
     2761 aatccacaat tgggtccgga tgcaactggg ctaggatggg tttatcagta tgtattaaac
     2821 tcagataaaa aagatttaca ggaacttcgt tcaattcagg attatttttt aaagtacgaa
     2881 ctctcttcta ttcagggagt tgctgaagtt gcaagcatag gcggttatgt aaaacaatat
     2941 caggtaaatc ttgatcccat taaacttgca tcatataaca tccctcttca aaaagttaaa
     3001 atggcaatca agcaaagcaa caacgatgtt ggcgggcgat tgcttgaaat gggtgaaatg
     3061 gagtttatgg ttagaagcct tggctacata aaaagcaagg aagatttaga agacgttgca
     3121 ataggagtct cgccaaattc aggttcaccg atatatctaa aagacgttgc cgatatttca
     3181 atcggacccg aactaaggag aggcctggct gactggaatg gagagggaga aactgttggt
     3241 ggaattattg taattcgtta tggcgaaaat gcccttgcag taattgacag agttaaagag
     3301 cgtttggaag aattaaaaaa atctctacct gctgatgtaa ctattgatgt tgcttatgat
     3361 cgttcaggac ttataaacag atcaatcgat aatctgtcaa acaaactaat tgaagaatct
     3421 ctcattgttg ctttagtgat aattgctttc ttgctgcacg ttagaagttc gtttgttgcg
     3481 atatttacac tgccgactgc cgttctcatt tcatttttaa ttatgagact gcagggtatc
     3541 aatgcaaaca tcatgtcgct tggcggaatt gcaattgcga taggtgcaat ggtggacgct
     3601 tcaatcgtga tggttgaaaa tgctgctacg catctctctt cggaaaacga taagcctccg
     3661 catgaaagaa aatcacactg ggcgacaatt cttcaatcgg caaaagaagt tggaccctca
     3721 atattttatt cgcttgttgt aattacaatt tcattcctgc ctgttttcac tcttgaacag
     3781 caggaaggca gaatgtttag acctcttgca ttcacaaaaa cttatgcaat ggctggtgct
     3841 gctattcttg ctgtaacaat agttccgatt ctcattggat attttgttcg aggaagaatt
     3901 cgaaaagaag aagataatcc aattacaaaa attctagtaa aaatttatca cccaattgtt
     3961 gattttgtta tgaagagaag atggtgggtg atggcgtcag cactgataat cgtacttatt
     4021 acaatacttc cattttcaaa acttggttct gagtttatgc ctccattata tgaaggcgat
     4081 ctgctgtata tgccgacaac attaccagga atatcaatta cgaaagcaag agaactttta
     4141 cagcagacag ataaaatcat aaaatcattc ccggaagtgg aatctgtttt tggaaaaatt
     4201 ggtcgggcag
//
LOCUS       DASWBY010000302         9766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_312565, whole genome shotgun sequence.
ACCESSION   DASWBY010000302 DASWBY010000000
VERSION     DASWBY010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312565"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VF870_05650"
     CDS             complement(<1..302)
                     /locus_tag="VF870_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9251704.1"
                     /translation="MSEFSQTEPRVYVTTAAKEKRKRINSFLIHIGLFFVTFITTTLA
                     GVEWTTGKLPPYEFYMLANGLPYSISILLIITFHEFGHYFAARFHRIRATLPFYIP"
     gene            complement(400..1347)
                     /locus_tag="VF870_05655"
     CDS             complement(400..1347)
                     /locus_tag="VF870_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HEX9251705.1"
                     /translation="MKNEKLKLSLQRSIQPVRLRNLALGLRSLPSVEMTGMKNKLLFA
                     IILFQLSFPSLTFAQFEHVLFPSDLNIRPFTANFLEPKAGFLFSLDSNKIRLDISTSR
                     DIVRWYDDDETISIGADLFTFTRLRSTDDFKFPVETIDYLFGLNAGYKKIVGQNNEFG
                     LRFRLSHISAHLVDGQYDAQAKEWREGREPFVFSKEFIQLFPYYRINTLRFYTGFTYI
                     FHIIPENIKTFNLQLGFDYFVTNFGNDDMAPFIAYDFKLAGNEKYVGNNIFSVGVKFG
                     KWDKGGLSLYYTYISGKSVHGEYYDLNENYSAIGFNFEL"
     gene            complement(1354..2349)
                     /locus_tag="VF870_05660"
     CDS             complement(1354..2349)
                     /locus_tag="VF870_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9251706.1"
                     /translation="MLLLCNYYVTYRCNAYCEFCHFGDHTNFKDTPYASLEDFKSNVT
                     QLAELGVKFIDLTGGEPLLHKDIAEMAKFAHDLKMQTSITSNGLLYHKFAEKLSGNIN
                     LLHFSLDSPDEEEHNKIRKVDCYKSVFKSIDIAKSLGEFPDILFTVTNDTYKKLPQMH
                     EIAQQHDLVLLVNPVFSYFGNPGLSDEATDYVDQYCDGKIDIYLNKGFMKLRRDGGND
                     INNPSCTAVSRVIVISPTNEIILPCYHFSNDKIEIDRPIKEIRQSEKIKHFMKMEGRF
                     DFCQGCTVNCYFEPSFAFPTNLYAISSLTSKFKYSYNKLIKQKIKKKFTQASNKE"
     gene            complement(2349..3440)
                     /locus_tag="VF870_05665"
     CDS             complement(2349..3440)
                     /locus_tag="VF870_05665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9251707.1"
                     /translation="MLELIFFFIMIGYFFQSFIFIIGARKKFPRIPDDRLPTATIIVA
                     ARNEEEKILRTLQSLDKLEYPEGKLQIMIVDDQSTDKTGKIIDDFIAGKSRFQKITTE
                     EHHSKLIGKMRALAYAIKEATGEIILTTDADCEVKPLWTKTMCSHYQNDVALVSGVTT
                     QTSEKWFHGMQAIDFVYLLTAGAGTTNLNVPISCIGNNMSFRKSAYDEVGGYEKLPHS
                     VTEDFTLMNAIYNLKKYKVIFPVDSDSLITSIPCRSLKSLVSQKKRWGVGGLGVPFRG
                     FIIMFWGFTANLLVLLTPFFFSSTWLTLAVFKVALDFFLLYPVHVKLGISKNLKYFFH
                     FEVYYLIYVILLPFIVLPNKKVIWKGREY"
     gene            complement(3428..4063)
                     /locus_tag="VF870_05670"
     CDS             complement(3428..4063)
                     /locus_tag="VF870_05670"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9251708.1"
                     /translation="MKYKYDPPFLVKKIFKDFSWNTENGKVLLTFDDGPNPGTTDIIL
                     KKLSDEKIKALFFCVGENVQKYPEFVQQIKDAGHTFGNHTFNHKILNKISEDEKDFQI
                     SSVNKIFKDQFDIKLKYFRPAHGRFQLSTSSFMRKYQIKNVMWSLLTYDYKNDFSIVK
                     FAVNKYLNHNSIIVLHDSNKSKNIISDSISFIIDEVRKRNYHFGEADECLN"
     gene            4150..5010
                     /locus_tag="VF870_05675"
     CDS             4150..5010
                     /locus_tag="VF870_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9251709.1"
                     /translation="MHNISEHNKGIFAVFLTAILWSSGGLFIKLISLDSMELSFFRCA
                     IAAIVFALMFRKRILKLNLLALLNSLAYAAVLILFVISTKTTTAANAIFLQSTAPIYV
                     LIFEPLLTKTKWARINIITIAVCFLGMILFFMGDLTPGDIKGNIAALLAGIAFATFFL
                     GMKKNDPQYGESSIFYGNVIVAIFCIPFVTDLTSISMQDFAMVSFLGIFQIAFAYALF
                     SYGLKRIIAVEASIISMFEPVLNPIWVFIGYGEVPSFYAIVGGIIIITAISTRTVISN
                     SDFIRTKFRF"
     gene            complement(5007..5651)
                     /gene="pdxH"
                     /locus_tag="VF870_05680"
     CDS             complement(5007..5651)
                     /gene="pdxH"
                     /locus_tag="VF870_05680"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX9251710.1"
                     /translation="MQEKNQLKNMRLNYDQDQLLESKISENPFTQFKIWFDLLLKADI
                     TEPNAMTIATSTKAGMPSARMVLLKDFDESGFTFFTNYGSRKGKELSESPFASMLFWW
                     REFERQVRIEGKIEKISRKESEEYFNLRPLKSRYGALASDQSEIVESREILEKKFADL
                     EKQFGDNPPMPENWGGYKLIPNKFEFWQGRRDRLHDRICYEKDKDCWKIYRLQP"
     gene            5788..9411
                     /locus_tag="VF870_05685"
     CDS             5788..9411
                     /locus_tag="VF870_05685"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX9251711.1"
                     /translation="MKPEKLTKKQKEELEKFGANTWFVESLYKQYEQKPDEVSEQWKN
                     FFGNVENKNKPNGDKVNESTGFLTLPKNIEMPKPGTEDEVKVIAGSSQRILDNMSSSL
                     TIPVATSQRTIAVKLLEENRIIINQHLQRKKEGKISFTHIISWAILKGISKVPSMNNG
                     FTIINGKPNIIIRKKVNLGLAIDIERKDGTRSLLVPNIKSANKLNFKEFWQAYEDIVV
                     RSRKGTIDPAEFLGTTVTLTNPGTIGTVSSIPRLMIGQGAIIATGAIQYNAEYQAMSS
                     ATISSLGISKVMNITSTYDHRIIQGAESGMFLREINELLLGKDGFYEEIFSSLNVPAR
                     PMQWETDYQPGGFDNPASTEEIIKQAKVLQLINMYRVRGHLVADLDPLGTRSVYHPEL
                     DPSSFNLTVWDLDRHFVTGGFSSLKTATLRQVINILQKTYCDKIGVEYMHIQNPAEKV
                     WLQSKMEPFQNSPKQNNEVKKNILDKLIKAESFENFVHTKFIGHKRFSLEGSETLIPV
                     LDKILNDSAENGISEIVIGMSHRGRLNVLTNILGKSYESIFSEFEDIKDKDSIQGSGD
                     VKYHLGATGMYPTNSGKELTISLASNPSHLEWVDPVVEGIVRAKQTRLRDHISHQKVI
                     PILIHGDAAFAGQGVVAETLNLSQLSGYRTGGTIHIIVNNQIGFTTTPEDSRSTTYAT
                     DVAKMIQAPIFHVNGDDPEAALWITQIALEYKLIFKKDVVIDLFGYRRHGHNEGDEPG
                     FTQPLLYDKIKSHPSVRKIYEEKLINDGVVTEEESKQMHNTFAEELTKSFDSLKRRSA
                     VFTNEAPLAVSKKELLSIQKESSTSISEEMLRDIIYALSTLPAGFNGHPKLEKFLEKR
                     KKLIDGSEMADWALAESLAFGSLLLEGTPVRLSGQDSVRGTFSQRHIAFTDIKTGKEY
                     FPLNHISDNQARLEALDSSLSEAAVLGFEYGYSIADPLALVLWEAQFGDFANGAQIII
                     DNFIVASYEKWGLPNGVVMLLPHGYEGQGPEHSSARLERFLILCAEDNMQVCNITTPA
                     QYFHILRRQTRETLQKPLVIMTPKSMLRLPEAKSPKENFISGKFEEMLDDETIRNKNS
                     INKIILTSGKVFYDLTKYRTKNNINDTAIVRIEQFYPFNKQRIIEIIKSYPNAQNIIW
                     LQEEPKNMGAWNFLSGRINELLSDNQKLICVSRPEGASPAVGSGKLSNQQQIELVENA
                     FKI"
     gene            9575..>9766
                     /locus_tag="VF870_05690"
     CDS             9575..>9766
                     /locus_tag="VF870_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251712.1"
                     /translation="MEEFQRQVANDFIIERVNLSQATISEAVRFKKHLETDIELGYNI
                     IIIDLSNCNSLDSAFIGVMV"
BASE COUNT         3266 a         1659 c         1681 g         3160 t
ORIGIN      
        1 aatggaatat aaaatggaag tgttgctctt atacgatgaa atcttgctgc aaagtaatgc
       61 ccaaactcgt gaaaagtaat tatcagaaga atactaattg agtaaggtaa tccattagcc
      121 agcatataaa attcatacgg cggaagtttt ccggttgtcc attcaactcc ggctaaagtt
      181 gtagtaataa aagttacaaa aaataatccg atatggatta gaaaactgtt aattcgctta
      241 cgcttctctt tagcagctgt tgtaacataa actcttggtt ctgtttgcga aaattcactc
      301 atatatgctt tttaatccag gtattaatca ctcttagttc atttcgaatc caacttttca
      361 gccggtcgaa atgactttac attataaaat aatttttatt tataattcaa aattaaatcc
      421 gattgcagaa tagttttcat ttaaatcgta gtattctccg tgaacacttt ttcccgaaat
      481 gtaagtgtaa tacaaactca atccgccttt atcccatttc ccaaatttta ctccgactga
      541 aaatatatta ttgccaacat atttttcatt accggctagt ttaaaatcat aagcaatgaa
      601 tggtgccata tcatcattac cgaaattagt aacaaagtaa tcaaatccta actgaagatt
      661 gaatgttttt atattttcag gaataatatg aaagatgtat gtaaacccgg tataaaatct
      721 taatgtgtta attctgtaat atggaaataa ctgaatgaat tctttactaa agacaaatgg
      781 ctctcttccc tctcgccatt cttttgcctg agcatcatac tgtccatcaa caagatgtgc
      841 gctgatatga ctcaatctaa accttaaacc aaattcatta ttctgtccga ctattttttt
      901 ataaccggca ttcaatccaa agagataatc gattgtctca acaggaaatt taaagtcatc
      961 tgtacttctt aatctggtaa acgtaaaaag atcagcaccg attgatattg tttcatcatc
     1021 atcataccat cttacaatat ctcgcgaggt tgaaatatcg agtcgtattt tgttactgtc
     1081 taatgaaaac aaaaatcctg cttttggctc aagaaagttt gctgtaaaag gtcggatgtt
     1141 taaatccgat ggaaatagta cgtgctcaaa ttgcgcaaat gttaatgatg gaaaggacag
     1201 ctgaaataaa attatagcaa aaagaagttt atttttcata cctgtcattt cgaccgaagg
     1261 taaagatctt aaacctaacg ccagatttct cagtctgaca ggttggatac tcctttgtaa
     1321 tgacaatttt aatttttcat tcttcatttt taattattct ttgttggatg cctgagtaaa
     1381 tttcttttta attttttgct ttataagctt gttataactg tacttgaact ttgaagttaa
     1441 actcgatatt gcatatagat ttgtgggaaa agcaaatgaa ggttcaaaat agcaattaac
     1501 agtgcagccc tgacaaaaat caaatctgcc ttccatcttc ataaaatgtt ttatcttttc
     1561 tgattgacga atttctttta ttggtctgtc aatttcaatt ttatcattag agaaatgata
     1621 acaaggtaag attatttcgt tagttggaga gataacaatt acccgagaaa cagctgtgca
     1681 gcttggatta ttaatatcgt tgccgccatc acgtcttaac ttcataaatc ctttgttaag
     1741 atagatgtca atcttgccgt cacaatactg atcaacataa tcagttgctt catcactcaa
     1801 gccgggattt ccgaaataag aaaaaaccgg attaaccaat aaaactaaat cgtgctgttg
     1861 ggctatttcg tgcatttgtg gaagcttttt ataagtatca tttgtaacag taaaaagtat
     1921 atcgggaaat tcacccagcg attttgcaat atcaatactt ttaaaaactg atttgtagca
     1981 atcaactttt ctaattttat tatgctcttc ttcatcagga gaatctaaag aaaagtgaag
     2041 cagattaata tttccagaca gtttttccgc gaacttatga tacaacaatc cattactcgt
     2101 aatgcttgtc tgcattttca aatcgtgagc aaacttagcc atctcagcaa tatctttatg
     2161 aagcaaaggc tcaccgcctg ttaaatcaat aaacttaaca ccaagttcag caagctgagt
     2221 tacattactt ttaaagtcct cgaggctggc atatggcgta tctttaaaat tggtatgatc
     2281 cccaaaatgg cagaactcac aataagcatt gcatcgatat gtaacgtaat agttgcagag
     2341 aagcagcatt aatattctcg acccttccag atcacttttt tattcggtaa aacaataaac
     2401 ggaagaagta ttacataaat taagtagtaa acttcaaagt ggaagaagta ttttagattt
     2461 tttgatattc caagttttac gtgtacagga tacagcagga aaaaatccaa agctacttta
     2521 aacacagcca aagttaacca tgtggatgag aaaaagaaag gtgttaataa tactagcagg
     2581 tttgcagtaa aaccccaaaa cataattata aaaccacgga acggaactcc aagtccgccc
     2641 acgccccagc gttttttctg acttaccaaa ctttttaatg agcggcaggg tattgatgtt
     2701 attaatgaat cagaatctac aggaaaaata actttgtatt tttttagatt gtaaatagca
     2761 ttcatcaatg taaaatcttc tgtaacgctg tgcggcagtt tttcatatcc gccgacttca
     2821 tcataagcag attttctaaa agacatatta tttccaatac aggaaattgg aacattaagg
     2881 tttgttgtac cggcaccagc tgttaaaaga taaacaaaat caattgcctg catgccatga
     2941 aaccactttt ctgatgtttg agttgtaact ccgcttacca aagcaacatc gttttgataa
     3001 tgagaacaca tggttttagt ccaaagaggt ttaacttcac aatctgcatc ggtggtaagg
     3061 ataatttctc cggttgcttc tttaatagcg tatgctagcg cacgcatttt tccaataagt
     3121 tttgaatgat gttcttcggt cgtgattttc tggaatcttg atttacccgc aatgaaatca
     3181 tcaatgattt ttcctgtttt atctgttgat tgatcatcaa ctatcattat ctggagtttc
     3241 ccttcagggt actcaagttt atccaatgat tgaagtgtgc gaagaatttt ttcctcttca
     3301 tttcgtgctg ctacaattat tgttgcagta ggaagtctat catcaggtat tctgggaaat
     3361 ttttttctcg ctccgattat aaagataaac gactggaaaa aatagccaat cattataaaa
     3421 aagaatatca gttcaagcat tcgtctgctt ctccaaaatg ataatttctt ttccttactt
     3481 catcaattat aaaactgatt gaatctgaga tgatattttt tgatttgttg ctgtcgtgca
     3541 aaactataat tgagttatga tttaaatatt tattgacggc aaatttaacg attgaaaagt
     3601 catttttata atcgtacgtg agcaaagacc acattacatt ttttatttga tactttctca
     3661 taaaagatga agttgataat tgaaatctgc cgtgtgccgg acggaaatat tttaatttaa
     3721 tatcaaattg atctttaaag attttgttaa ctgaagaaat ctggaaatcc ttttcatcct
     3781 cagaaatttt atttaaaatt ttatgattaa atgtatgatt accgaaagtg tgaccagcat
     3841 ctttgatctg ttgaacaaat tcaggatact tttgaacatt ttcaccgaca caaaaaaaca
     3901 gagcctttat cttttcatca gaaagctttt ttagaattat gtctgttgtt cccgggtttg
     3961 gtccatcatc aaaagttaat agaaccttgc cgttttcagt attccagcta aagtctttaa
     4021 atattttttt aactaaaaaa ggcggatcgt acttatactt catttatttg tacagactga
     4081 ttattcaatt aaatcttttt ctgtaattta gtcatagtaa aataaattca gtaatattta
     4141 tagctttacg tgcataatat ttcagaacat aataaaggca tatttgcagt tttccttact
     4201 gctattctat ggagttcagg cgggcttttt ataaaactaa tttccctgga ctcaatggag
     4261 ctttcctttt tccgctgcgc aattgcagcg attgtatttg cgcttatgtt cagaaagaga
     4321 attttaaaat taaatctatt agcgttactc aattcactag catatgccgc agttttaatt
     4381 ttatttgtaa tttcaacaaa aactaccaca gcagcaaatg cgatatttct gcaatcaaca
     4441 gcgccaattt atgttttaat atttgaacca ttacttacaa aaacaaagtg ggcaagaata
     4501 aacataatta caattgctgt ttgtttttta ggaatgattt tattttttat gggggatcta
     4561 actccaggtg atattaaagg aaatattgca gcattattgg ctggaattgc ttttgcaaca
     4621 ttctttttgg ggatgaaaaa aaatgatccg cagtacggcg aatcatcaat cttttatggt
     4681 aatgtgatcg ttgctatttt ctgtattccg ttcgtaacag atttaacttc aatatcaatg
     4741 caagattttg caatggtaag ttttcttggg atttttcaaa tcgcatttgc ttatgctctt
     4801 ttcagttatg gattaaaaag aattattgct gttgaagctt caatcatttc gatgtttgaa
     4861 ccagtgctga atccaatctg ggtttttatc ggatatggag aagtgccatc tttttatgca
     4921 atcgttggag ggataataat tataactgca atttccaccc ggacagtaat ttcaaattca
     4981 gattttataa gaacaaagtt taggttttaa ggctgtaacc tgtaaatctt ccaacaatct
     5041 ttatcttttt cataacaaat tctatcgtgt aatctatccc gcctaccctg ccagaactca
     5101 aatttatttg gaatgagttt atatccgccc caattttctg gcatcggtgg attatcaccg
     5161 aactgttttt ctaaatcagc aaatttcttt tctaatattt ctcgactctc aactatttca
     5221 ctttggtctg aagccaatgc tccatatcta cttttaagtg gacgcaggtt aaaatattct
     5281 tcagattctt tcctgcttat tttttcaatc ttcccttcta ttcttacctg tctttcaaac
     5341 tctctccacc aaaaaagcat ggatgcaaat ggactctctg aaagctcttt tccttttctg
     5401 cttccgtaat tagtaaagaa tgtaaatccg ctttcatcaa aatctttaag taagaccatt
     5461 cttgcagaag gcattccggc ttttgtcgaa gttgcaattg tcatcgcatt gggttctgtt
     5521 atatctgctt ttagaagaag atcgaaccaa attttgaatt gggtgaatgg attttcactt
     5581 attttagatt caagcaattg atcttgatca taatttaagc gcatattctt taattgattt
     5641 ttttcctgca tagatttttt gtattaatgt cttatatttt cggcactaaa tccgattaaa
     5701 ttttgaataa taaattagat agttaattaa ctatctaatt gaaaaataaa cttattagaa
     5761 tttgtaaaca aaactccagc taaaaaaatg aaacctgaaa aactgactaa gaagcaaaaa
     5821 gaagaattag aaaagttcgg tgcaaacaca tggtttgtag aatccctcta taaacaatat
     5881 gaacaaaaac ctgacgaagt ttcagaacag tggaaaaact tttttggaaa tgttgaaaat
     5941 aaaaataaac cgaatggtga taaggtcaat gaaagtacag gattcttaac acttccgaaa
     6001 aatatagaaa tgccaaaacc cggtaccgaa gatgaggtta aggttattgc cggtagttca
     6061 cagcgaatac tggataatat gagctcaagt ttaacaatcc ctgttgctac atctcaaaga
     6121 actattgctg taaaactact tgaagagaac agaattatta taaaccagca tctgcagaga
     6181 aaaaaagaag gaaagatttc atttactcac ataattagct gggcaatttt aaaaggtatt
     6241 tctaaagttc cttcaatgaa taatggtttt acaattatta atggcaaacc aaatatcatc
     6301 attcgtaaaa aagtaaatct tggtttagcg attgatattg aacgaaaaga tgggacccga
     6361 tcacttcttg ttccgaacat aaagtcagca aataaattaa actttaaaga attctggcag
     6421 gcctatgaag atatcgttgt gcgatcaaga aaaggaacaa ttgatcctgc agaattttta
     6481 ggaaccacag ttacacttac aaatcccggc acaattggaa cagtttcctc aatcccacgt
     6541 ttgatgattg ggcaaggggc aataattgca actggagcca ttcagtacaa tgctgaatac
     6601 caggcgatgt cttcagccac aatttcttct cttggaatca gtaaagtaat gaacataaca
     6661 agcacttatg atcatagaat aattcaaggt gcagaatccg gaatgttctt gcgtgagatt
     6721 aatgaattac ttttaggtaa agatggattt tacgaagaga ttttcagttc tttaaatgtt
     6781 cctgcccgcc cgatgcaatg ggaaacagat tatcagccag gcggatttga taatcctgcc
     6841 agcacagaag aaataattaa acaggcaaaa gttttacagc tcataaatat gtacagagtt
     6901 cggggacatt tagtggcaga tcttgatcca ttaggtacac gctctgttta tcatcccgaa
     6961 cttgatccat cttcatttaa tcttacagtt tgggatttgg atagacattt tgttaccggt
     7021 ggttttagct ctttaaagac agcaacgctg cgacaggtaa ttaatattct gcaaaaaact
     7081 tattgcgata aaattggtgt ggaatatatg cacatccaaa atcctgcaga aaaagtctgg
     7141 ctgcagagta agatggaacc atttcagaac tctccaaaac agaataatga agtaaagaaa
     7201 aatattctgg ataaattaat taaggctgag tcttttgaaa attttgtaca tacaaaattc
     7261 atcggacata aaagattttc tttagaagga tctgaaactc taatccctgt gctggataaa
     7321 attctaaatg attctgcgga aaatggcatc tcggaaattg taattggaat gtctcatcga
     7381 ggcagattaa acgttcttac gaatatcctt ggtaaatcgt atgaatcaat cttcagtgaa
     7441 tttgaagaca taaaagataa agactccatt caaggatcag gtgatgttaa atatcatctt
     7501 ggtgcaacgg gtatgtatcc aaccaattcc ggaaaagaat taacaatttc acttgcttct
     7561 aatccaagcc atctggaatg ggttgatccg gtcgtggaag gaatagtccg tgcaaaacaa
     7621 acacgacttc gtgaccatat atctcatcaa aaagttatac cgattttaat tcatggtgat
     7681 gcggcttttg caggacaagg agttgttgct gaaacattaa atttatcaca gcttagcggt
     7741 tatagaaccg gcggaacgat tcacataatt gtaaataatc aaattggttt tacaactact
     7801 ccggaagatt ccaggtcaac cacttacgct acagatgttg caaagatgat ccaggcacca
     7861 attttccatg tcaatggcga tgaccctgag gcagcactat ggataacaca aattgcatta
     7921 gagtataaat taatctttaa aaaagatgtt gtaatagatt tattcggata tagacgacac
     7981 ggacataatg agggtgatga gcccggcttc actcaaccat tgttgtacga taaaataaaa
     8041 tcacacccat cagttagaaa aatctatgaa gaaaaattga tcaatgatgg tgtggtcact
     8101 gaagaagaat caaaacaaat gcataataca tttgcagaag aattgactaa gtcctttgat
     8161 tcacttaaac gcagaagcgc cgtgtttaca aatgaggctc cgcttgcagt ttctaaaaag
     8221 gagttgctgt ccatacaaaa agagagcagc acttctatat cagaagaaat gttgagggat
     8281 attatttatg cattatcaac tcttcctgct ggattcaacg ggcatccgaa attggaaaaa
     8341 tttttagaga aaagaaaaaa acttattgat ggatccgaaa tggcggattg ggccttagct
     8401 gagtcgctgg cattcggtag tcttttacta gaaggaactc cggttaggtt aagcggacag
     8461 gacagcgtta gaggaacttt cagccagcgg catattgcat tcacggatat caaaacagga
     8521 aaagaatatt tccctttaaa tcatatttca gataatcaag cgagattaga agcattagac
     8581 agttcattat ccgaagctgc ggtattggga tttgaatatg gctacagcat tgctgatccg
     8641 cttgctttgg ttttatggga ggctcagttt ggcgattttg caaacggtgc tcaaataatt
     8701 attgataatt ttatagttgc atcttacgaa aaatgggggc ttcctaacgg agttgtaatg
     8761 ctgctgcctc atgggtatga aggacaagga ccggaacact ccagcgcacg tttagaaagg
     8821 tttttgatct tatgcgcgga agataatatg caggtttgta atatcacaac tccagcacag
     8881 tattttcaca tcttaagaag acagacaagg gaaactttac aaaaaccttt ggtgataatg
     8941 actcccaaaa gtatgctgag attgcccgaa gcaaaatcac caaaagaaaa ttttatatca
     9001 ggtaagtttg aagaaatgct tgatgatgaa actatcagga ataaaaattc tataaataaa
     9061 attattctta ccagcggaaa agttttttat gatttaacca agtatagaac taaaaataat
     9121 attaatgata ctgcaatcgt aaggattgag cagttttatc catttaacaa acagagaata
     9181 attgaaataa taaaatctta tccaaatgca caaaatatta tttggctgca ggaagaaccg
     9241 aaaaatatgg gagcctggaa ttttcttagc ggaagaataa atgaactatt atctgataat
     9301 caaaaattga tttgtgtaag ccggcctgag ggtgcaagtc ccgcagttgg atctggaaaa
     9361 ttatcaaatc aacagcaaat agaacttgta gaaaatgctt tcaaaattta acaattgata
     9421 aatgttcatt tcctatcgac acaacctcat tatgaggatg tatctgtcta ttgttttaat
     9481 ctgaaacaca aacttcttcg attagggcaa aaataactca tttctaatgg catattaatt
     9541 ggccttttaa cgaccaataa aattgagaat ggaaatggaa gagtttcaaa gacaagttgc
     9601 gaatgatttt ataatagaaa gagttaatct ttctcaagct acaatttcag aagcagtgcg
     9661 gtttaagaaa catcttgaaa ctgacattga acttggctac aacattatta ttatagatct
     9721 aagtaattgc aactcacttg attcggcttt tataggggtt atggtt
//
LOCUS       DASWBY010000303         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_649205, whole genome shotgun sequence.
ACCESSION   DASWBY010000303 DASWBY010000000
VERSION     DASWBY010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649205"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1357)
                     /locus_tag="VF870_05695"
     CDS             complement(92..1357)
                     /locus_tag="VF870_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251713.1"
                     /translation="MIQSESIVSAYAPPFKIVAKYFIAAIVSFVLLNFLLLLNYSEIV
                     GHHFNPKILSINHVATLGWITMIIFGAMFQLVPVVLETKLFSEKLAEIQFWIYLPGVI
                     GLVYCFWVFDTGINMTFSAVLLNLAMFIFSFNIIITMKSVKKWNVTAWYLAAAIFYLI
                     VTAIAGLLLAINLWTPYVKIDHLQYLNLHAHIAFIGWVSMVIMGVSFKLIPMFTLSHG
                     FPLTNAKLAFWILNVGLLGISTIMHYKDTTFLYYIFTGLIVLGIIFFLLQIRIIFKNR
                     IRKKYDIGIKFSVVAYLMLGLTTILGTFIAFVDYQNIINLTLIYGYMIIFGYISMLIV
                     GQMYKIVPFLVWYHKYSSKVGLEPVPMLKDMFNEKFAQIEFYLMITAVFGSLYSLAFG
                     SETGVLISFSIMFISSAIFLFNMITIFRK"
     gene            complement(1360..>1968)
                     /locus_tag="VF870_05700"
     CDS             complement(1360..>1968)
                     /locus_tag="VF870_05700"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX9251714.1"
                     /translation="SINADITETITEESTEEKMTQIEKPELLKNKSADKIQKMDVRSI
                     IDSGKDPFLEIMAKVKSLKDEEVFHLINSFEPIPLYSVMQNKGFEHWTENNGSVFNVF
                     FFKNDVNKSEVKVESNLSQVSPADFENVIELDVRELAPPEPMMKILENISRVDEKTVM
                     VVHHHREPMMLYPKLEERGYTAITNKIDENYFKVVIMKKRND"
BASE COUNT          677 a          342 c          314 g          635 t
ORIGIN      
        1 gagcccaaga tcaacgatat taattcctat ctcaggatca ataacacctt ttagaacttc
       61 caatatttct tctttgtttg ccatatagtt tttacttcct gaaaattgtt atcatattaa
      121 atagaaagat tgcagaactg ataaacatta ttgaaaatga aatcagtacc cctgtttcac
      181 ttccaaatgc taaagagtaa agagagccga aaactgccgt aatcattaaa tagaactcaa
      241 tctgtgcaaa cttttcattg aacatatctt ttaacatcgg caccggttca agcccgactt
      301 tactgctgta tttgtggtac caaacaagaa atggtacaat cttgtacatc tgtccaacaa
      361 taagcataga gatgtaaccg aagattatca tataaccgta aataagtgta aggttgataa
      421 tgttttgata atcaacaaat gcaataaaag ttccgagtat ggttgttagt ccaagcataa
      481 gatatgcaac aacagaaaac ttaataccta tatcatactt ttttctaatt ctgtttttga
      541 aaatgatacg aatttgaaga aggaagaaaa ttatccctaa aacaattagt ccggtgaata
      601 tgtaataaag aaacgttgta tccttgtaat gcataatagt agatatccca agtaagccta
      661 cgttaagaat ccagaaggct agttttgcat ttgttaaagg aaaaccatgt gaaagagtaa
      721 acattggaat aagcttaaat gaaactccca ttatcaccat agaaacccag ccaatgaaag
      781 caatgtgtgc gtgtaaattt aaatattgaa gatgatctat ctttacgtat ggtgtccata
      841 gatttatagc cagcaaaagt cctgctatcg cagttacaat caaatagaaa attgccgctg
      901 caaggtacca cgcagtaacg ttccatttct taacgctttt catagtaatg attatattaa
      961 acgaaaagat gaacattgca agattaagta gtactgctga aaatgtcata ttaattccgg
     1021 tatcgaatac ccagaagcaa tagactaaac cgataacgcc aggcagataa atccagaact
     1081 gaatctccgc gagtttttca ctgaacaatt ttgtttcgag aactacagga acaagttgaa
     1141 acatcgcacc gaatattatc atcgttatcc aaccgagtgt tgcaacgtga ttgattgaaa
     1201 gaattttagg attaaaatga tgtccaacta tttcagaata attaagaagt aaaaggaaat
     1261 ttaaaagcac aaacgaaaca atggctgcga taaaatattt tgcaactatt ttgaatgggg
     1321 gtgcataagc agagactatt gattcggatt gaatcataat taatcgtttc tctttttcat
     1381 tattacaacc ttaaaataat tttcatcaat cttatttgta attgctgtgt aacctcgttc
     1441 ctcaagtttt ggataaagca tcatcggttc acgatgatga tgaacaacca taactgtttt
     1501 ctcatcaact ctggaaatgt tttcaagtat cttcatcatt ggttcgggcg gagctaattc
     1561 acgaacatca agttcgatga cattttcaaa atcagcagga gatacctgac ttaggttcga
     1621 ttctactttt acttcagatt tatttacatc atttttgaag aagaagacat taaacacact
     1681 gccattattt tctgtccagt gctcaaaacc tttattctgc attactgaat aaagcggaat
     1741 tggttcaaac gaatttatca aatgaaaaac ttcctcatct tttagagatt ttacttttgc
     1801 cattatctca aggaaaggat cttttccaga atcgattatc gatcttacat ccatcttttg
     1861 aattttatca gcacttttgt tttttagaag ttcaggtttt tctatttgag tcattttttc
     1921 ttccgtgctt tcttcagtaa tagtttcagt aatatctgca ttgattga
//
LOCUS       DASWBY010000304         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_536665, whole genome shotgun sequence.
ACCESSION   DASWBY010000304 DASWBY010000000
VERSION     DASWBY010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536665"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3115
                     /locus_tag="VF870_05705"
     CDS             <1..3115
                     /locus_tag="VF870_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9251715.1"
                     /translation="KREEIIGVNIFESGFLTSKSAVTSAFYLSTLLMKKEVPIFEINA
                     IKKDSNLITYELRGVPIQEKDDLIGVQVILRNVTERKKTEAKLFQNEKQLSNLMSNLP
                     GMAYRCKYDQDWTMEFVSQGCLELTGYLSEELLFNSKISYAELIHPEDKENVFLTIRE
                     SIRKKAPYQIIYRIRTKEGAEKWVWEKGNAQTSRKGKVETLEGFITDISNRINAEEAL
                     KESEELYRKLIATLPDIIAITDIKGNIIFLNDIGVKFTGYNSFEEVKHQNFINFISKD
                     ERDKVTLNFKKVFLKNIGPQEYGFTNKNGENFLFEVQREILKTVDGSPYGLIFSCRDI
                     TARKKAELELSQSEEKYRTLIDSIQDGVFLIVDEIVQYVNRAFCKMIGYENNEVEGSS
                     FLKFIAPEDIELVSGNYKLRQEGKPAPSSYEWSMLHKNGGKVFVNMSARVINYQGKKA
                     TIGTIKDVTHQKKLEQTLLNQKNLFKGVADAANILLTEKDFNLAISNTLKSLGQSSDI
                     DRVYIFENSFESITGEPVMSQRYEWTNGNVSSEIDNPELQNLHYYPMFKSWYPILNEG
                     GIVNSLVKDLNTELRELLSEQNIKSILLVPILVKNNFWGFIGFDYCKADRIWSESEIS
                     ILQTTAANLSGVIEKELAKKELIEAKNTAEEMSKLKSNFLANMSHELRTPLIAILGYT
                     EILSTEIEHQEWNEMISTIMNSGKRLLETLNLILDLSKVEADKVQINYSEINISEEVN
                     DIVTLLSPIAQKKNLYLKAVVEKENILSKLDKRILHSIVTNLVNNALKYTHSGGVTID
                     LSLDKSIGKNYAVIRVIDTGIGIAKEDQETIFDEFRQVSEGYNRHFEGAGLGLTIAKK
                     FAEKMGGNISLQSEAGKGSTFTVVFPAEEETIIAEPIKNIARENNLPHSMGKSKKVLV
                     VDDDSATRKIVELFLRGEIELDSASNSKEASDMINNNFYSLILLDISLGKGISGVDLL
                     QEIKSIPAYKNVPIIAVTAHAMVGDKEKFLSSGFDDYLSKPFAKKDLVGKVHNWVSGA
                     RGNGNDY"
     gene            complement(3121..3723)
                     /gene="aat"
                     /locus_tag="VF870_05710"
     CDS             complement(3121..3723)
                     /gene="aat"
                     /locus_tag="VF870_05710"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX9251716.1"
                     /translation="MSAKEDQISDELLKPDNMLRLYATGAFPMADQESGSIEWYLPDT
                     RCIIPLNDFNIPRSTKKEIEKLNFEIRYDTDIVAVINGCADREHTWISERLIEAYKRL
                     IKRGHVHSVETWKDGMLVGGLYGVTFRGAFFGESMFSKTSQASKAALINLLYHLKEKD
                     FALLDVQYMTDHLKMFGAIEISLDEFKSHLLEAYQRICEF"
BASE COUNT         1480 a          583 c          727 g         1212 t
ORIGIN      
        1 taaaagagaa gaaattatag gcgtaaatat atttgaatct ggatttctaa cctcaaagtc
       61 tgcagtgacc tctgcgtttt atctttcaac attgttgatg aaaaaggaag tacccatttt
      121 tgaaattaat gcaatcaaaa aggattctaa tttaattact tatgaattga ggggagttcc
      181 gattcaggaa aaagatgatt taataggtgt tcaagtaata ttaagaaatg ttacagaacg
      241 caaaaaaaca gaagcaaaac tttttcaaaa tgaaaagcaa ttatctaacc tgatgtctaa
      301 tctgccgggg atggcatatc ggtgcaaata cgaccaagat tggaccatgg aatttgttag
      361 ccagggctgc ttagaattga cgggctattt atctgaagaa ttacttttta atagtaaaat
      421 ttcttacgca gaactgattc atcctgagga caaagaaaat gtttttctta cgataagaga
      481 atcaattaga aaaaaagctc cttatcaaat aatctatcga attagaacaa aggaaggtgc
      541 agaaaaatgg gtttgggaaa aaggcaatgc acaaacttca agaaaaggaa aagttgaaac
      601 attagaagga tttatcactg atatttcaaa ccggattaat gcagaagaag ccttaaagga
      661 aagcgaagaa ctttatagaa aactaattgc aaccctgccc gatattattg caattacaga
      721 tattaagggc aatataattt tcctaaatga cattggtgta aagtttacag gttacaatag
      781 ctttgaggaa gtaaaacatc agaattttat taactttatt tctaaggatg aacgtgataa
      841 agttacccta aactttaaaa aagtatttct aaaaaatatt ggaccacagg aatatggttt
      901 tacaaataag aacggggaaa attttctgtt tgaggtgcaa agagagatac taaaaacagt
      961 tgatggttct ccttatggtc tcatattttc atgtcgtgat ataaccgcaa gaaaaaaagc
     1021 agaacttgaa ctctctcaat ccgaagaaaa atatagaacg cttatcgata gtattcagga
     1081 tggtgttttc ttaattgttg atgaaatagt tcaatatgtt aacagagcat tttgtaagat
     1141 gattggatat gaaaataacg aggttgaagg cagttcattt ttaaaattta ttgcaccgga
     1201 agatattgaa cttgttagtg gaaattataa actaaggcag gaaggtaaac ctgctccatc
     1261 atcttatgaa tggagtatgc ttcataaaaa tggtggaaaa gttttcgtga atatgagtgc
     1321 aagagttatt aattatcagg gtaaaaaagc tacgataggt actatcaagg atgtaacgca
     1381 tcaaaaaaaa ttagagcaga ctttgctaaa tcaaaaaaat ctttttaaag gtgttgcgga
     1441 tgctgccaat attctattaa ctgaaaagga ttttaattta gcaatttcaa acactttaaa
     1501 atcgctagga caatcatcgg atattgatcg tgtgtatatt tttgaaaatt catttgaaag
     1561 catcaccgga gaaccggtta tgagtcagag atatgagtgg actaacggaa atgtatcttc
     1621 agaaatcgat aatccggaac ttcaaaacct tcactactat ccaatgttta aaagctggta
     1681 tccaatactg aatgaaggag gaatagttaa tagtctggtt aaggatctga atactgaatt
     1741 acgggaatta ctttctgaac aaaatattaa atcgattctt cttgttccta tattagtaaa
     1801 aaacaatttc tggggattta ttggatttga ctattgcaaa gctgatagga tttggagtga
     1861 aagtgagatc tcaattctgc aaacaactgc agcaaattta agcggagtga ttgaaaaaga
     1921 acttgcaaaa aaagaattga tagaagcaaa aaacacagcc gaagaaatga gcaaattaaa
     1981 atccaatttt cttgctaata tgagtcatga acttagaact ccattaattg caatccttgg
     2041 ctatacagag atattaagta ctgaaattga gcatcaggaa tggaatgaaa tgatttctac
     2101 gattatgaat agtggaaaga ggcttttgga aacattaaat ctgatccttg atctttcaaa
     2161 agtggaagca gacaaagttc aaatcaacta cagcgaaata aatatttctg aagaagtaaa
     2221 tgatatagtt acattgttaa gtccgattgc acagaaaaaa aatctatact taaaagctgt
     2281 tgttgaaaaa gaaaatattc tttctaaact tgacaaaaga attttgcatt caatcgtaac
     2341 caatcttgta aataacgctt taaagtacac tcacagcggt ggagtaacta ttgatttaag
     2401 cttagataaa agtatcggca aaaattatgc ggtgattaga gttatcgata caggtatagg
     2461 aatagctaaa gaagatcagg aaactatttt tgatgagttc agacaggtta gcgaaggcta
     2521 caatcgccat tttgaaggtg ctggattagg tcttacaata gctaaaaagt ttgcagaaaa
     2581 aatgggaggt aatatttcat tgcaaagtga agccggcaaa ggttctactt ttaccgtagt
     2641 gtttcctgca gaagaagaaa caataattgc agaacctata aaaaatatag caagagaaaa
     2701 taacctgccc cactcaatgg gtaaatccaa aaaagtttta gtagttgatg atgattccgc
     2761 tacaagaaag attgtagagc tttttttacg tggtgaaatt gaacttgatt cagcttcaaa
     2821 cagcaaagaa gcatcagata tgataaacaa taatttttat tcgcttatat tattagatat
     2881 aagtcttggt aaaggcatta gcggtgtgga tttacttcaa gaaataaaat ctattccggc
     2941 atataaaaat gttcccataa tcgccgtaac tgctcatgca atggttggtg ataaagaaaa
     3001 atttttatct tcaggctttg atgattattt atcgaaacca tttgcaaaga aggatttagt
     3061 aggtaaagtt cataattggg ttagtggagc taggggcaat ggaaatgatt attaatcaat
     3121 ttaaaactca caaattctct gataagcctc aagcagatgg gatttaaact catctaaact
     3181 tatttcaatt gccccaaaca ttttaagatg atcagtcata tactgaacat caagtaaagc
     3241 aaaatctttt tctttaagat gataaagaag attgattaat gctgccttgg atgcttgtga
     3301 tgtttttgaa aacatcgatt caccaaaaaa agcccctcta aatgtaacac cgtataaacc
     3361 accaacaagc attccatctt tccaggtttc cacagaatga acatgaccac gtttaattaa
     3421 tcttttgtat gcttcaataa gtctttcgga aatccaggta tgttcacggt ctgcgcatcc
     3481 attaattaca gcaacaatgt cagtgtcata acgtatttca aaatttaatt tttcaatttc
     3541 tttttttgta gatcttggaa tgttaaaatc atttagtgga ataatgcatc ttgtatccgg
     3601 taaataccat tcgattgaac cggattcctg atcagccatt ggaaatgcac ccgtagcata
     3661 aagtctcaac atattatccg gttttaacaa ttcatcagat atctggtctt ctttagcact
     3721 catttttatg acttaaatgc aaaatttcga ataatttttt atttgaaaaa taccttcttt
     3781 aagttaacaa ttcaattttt caaatgcttt gtaaacaaag ttattaaaaa agagttttat
     3841 gataaaaact gcagtagttg cacttggagg aaacgcgctt cttaggggaa atgaaatcgg
     3901 cactatccag caacaggaaa aaaacacata cgatacgtgt attagtcttt taaatcttct
     3961 taaagaaaat tacaatcttg taatcactca cggtaacggt cc
//
LOCUS       DASWBY010000305         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_649319, whole genome shotgun sequence.
ACCESSION   DASWBY010000305 DASWBY010000000
VERSION     DASWBY010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649319"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2560)
                     /locus_tag="VF870_05715"
     CDS             complement(<1..2560)
                     /locus_tag="VF870_05715"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HEX9251717.1"
                     /translation="MSIALFSSFILAQNIQSPNEDGYPLITNYLPKEYAANVQNWAVI
                     QDNRGVLYFGNVSGVLEYDGVNWRLIHVPNDVVRCFAIDNDGKIYVGGINEIGYLAPD
                     SMGALNYVSLNKYISKEKVSYGDVWSIIVHDNGLYFQTFSTLFLLESNKADEQCFFDR
                     ITQNPSLRKWESRTRMNPIFSIRKRIFVHERNIGLQEMINGKITLLPGGEEFAQDLIC
                     IMMPYPSIEKINDHSNKILIGSLRRGMFLFDGEKFEKFNNEADNYLIEKRLYFKGALL
                     RDGSYALGTQLGGIVIIDKDGKLKKIINKSIGLNNETIWNLFCDREGNLWAALDNGIA
                     KIIYPSALQIIDEKSGFEGLIQSIKSFGDKIYIATSSGIYYMQNSSTNSEVNKFIPVK
                     NISVQSWNFLSLKDKLLAATNDGVFAIKDNIASLIETDLRYTYCFCKSKSDESLIYIG
                     LHNGLGILKYENEKLTYLGSLVDFNNGITKMEEDTNGDLWCNDIAGKIIYLKTPADKS
                     DLRGYKKVQLSNENILGKKAKLFKQSDSIFYYNDKNIFSYSYQKNKFIFSDRFKEIIK
                     DSLQSFLNIYPDENNTTWYTINNQGKIQIKKVVNDVDTFYIKTHSLLGLIDEDLSSDF
                     ISIQTYVQKNQEQIIWISGVNILIRYNLVSDLSFNFSNQSRPLIRKVYLNGGENIFYG
                     YTNITDNSEEVSTNISYSSGSIAFEYSLTSFLNESGNDFQYMLEGFDKDWSGWTNQTK
                     KEYTNLSSGNYSFKVRSRNSIGQLSGEAVYIFRVLPPWYKTWWANLTGFLIVLLIINY
                     VIRFRVRYLKNKNIVLERLVSERTAKINEQKELLEKQAQKLVELDQIKSNFFANI"
     gene            complement(2723..>3142)
                     /locus_tag="VF870_05720"
     CDS             complement(2723..>3142)
                     /locus_tag="VF870_05720"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HEX9251718.1"
                     /translation="TALNNYGLAKQMDFGYVYDLNKKWNQELKAPAIPQVKDLYEYLL
                     KKGIKIIFLTGRNYTEYEVTYKNLIQAGYTKFDTLITQREEELNLKSQEFKSKKRTEL
                     TQQGYEIIGTVGDQWTDLNGPYSGIQIKIPNYLYEIK"
BASE COUNT         1000 a          531 c          445 g         1166 t
ORIGIN      
        1 atatgtttgc aaagaagttt gattttatct gatcaagctc aactaatttt tgcgcctgtt
       61 tttcaagcaa ttctttctgt tcatttattt tagctgttct ttcactaaca agtctttcca
      121 gaactatgtt tttgtttttt agatatctta ctctaaatct tataacataa ttaattatca
      181 gaagcacaat aagaaaacca gttaagttag cccaccaagt tttataccat ggaggaagta
      241 ctctaaaaat ataaacggct tcaccactca gctgtccaat ggaattacgg ctgcggactt
      301 taaatgaata attacctgat gaaagatttg tgtattcttt tttagtctga ttagtccaac
      361 cgctccaatc tttatcaaat ccttctaaca tatattgaaa atcatttccg gattcattga
      421 gaaaagatgt tagagaatat tcaaatgcta tggagccgga ggagtaacta atatttgtcg
      481 atacttcttc actgttgtca gttatatttg tatacccata gaagatgttt tccccgccat
      541 ttaaatatac ttttctaatt aatggtctac tctgattgga aaaattaaaa ctcagatccg
      601 aaactaaatt gtatcttatg agaatattta cgccgctgat ccagattatt tgttcttgat
      661 ttttctgaac atacgtctga attgaaatga aatcagaaga aagatcttca tctattaatc
      721 caagtaagga atgtgttttt atataaaagg tgtctacatc attaacaact ttctttatct
      781 gtattttgcc ctgattgttt attgtgtacc aggtagtatt gttttcatca ggataaatat
      841 ttaaaaaaga ttgtaaacta tctttaataa tttctttaaa tctatccgaa aaaataaact
      901 tgtttttttg ataagaatag ctaaaaatat ttttatcatt gtagtaaaag atgctatcgg
      961 attgtttaaa tagctttgct tttttaccca gtatattttc attactcaac tggacttttt
     1021 tataacctct taaatcactt ttgtcagcag gagtttttaa atatataatt ttccctgcta
     1081 tatcattgca ccacagatct ccattagtgt cttcttccat ttttgtaatg ccattattaa
     1141 aatccacaag agaacctaaa tatgtcaatt tttcattttc atattttaaa atacccaacc
     1201 cgttatgcaa tccgatgtaa attaaagatt catcagattt tgatttacaa aaacaatatg
     1261 tatatctaag atcggtctct attaaggatg cgatattgtc tttaattgca aacacaccat
     1321 cgtttgttgc tgcgagcaat ttgtctttga gtgataagaa attccacgat tgaactgaaa
     1381 tattttttac ggggataaat ttattaactt cagaattagt tgaagagttt tgcatataat
     1441 atatgcctga actggtagct atataaattt tgtctccaaa ggattttata gactgtatta
     1501 atccctcaaa gcctgatttt tcatctatta tctgcagtgc tgatggataa ataatttttg
     1561 caattccgtt atctagtgca gcccaaagat ttccttcacg atcacaaaat aaattccaaa
     1621 tagtttcgtt atttaagcca atacttttat taataatttt tttcaattta ccgtctttat
     1681 caattataac aataccgcca agttgtgttc caagtgcata acttccatcc ctaagtaaag
     1741 cccctttaaa gtaaagtctt ttttcaataa gataattatc agcttcatta ttaaactttt
     1801 caaatttttc cccatcaaat aaaaacatcc cccgccttaa agaaccaata agaattttat
     1861 ttgaatgatc gttgattttc tctattgatg gatatggcat cattatgcaa ataagatcct
     1921 gtgcaaactc ttcaccacct ggtaataatg taatttttcc attaatcatt tcctgcaacc
     1981 caatgtttct ctcgtgaaca aatattcttt tcctaataga aaatatcggg ttcattcgtg
     2041 ttcgggattc ccattttctt aaggatggat tttgcgttat gcggtcaaag aaacattgtt
     2101 catcagcttt attcgattcc agcaaaaaaa gagtggaaaa tgtttgaaag taaagcccat
     2161 tatcatgaac gattatactc catacatcac cataactaac cttttctttg gatatatatt
     2221 tattaagtga aacataattg agtgcaccca ttgagtcagg cgccaaatat ccaatttcat
     2281 ttataccgcc aacatatatt ttcccatcgt tatcaatagc aaaacatcga acaacatcat
     2341 tgggaacgtg aattaatctc caattaactc catcatattc aagtacacct gaaacatttc
     2401 caaaatatag aacacctcta ttgtcttgaa taacagccca gttttgaaca tttgcagcgt
     2461 attctttggg taaataattt gttattaatg gataaccgtc ttcatttggg gattggatat
     2521 tttgtgctaa aataaaagaa gaaaataatg cgattgacaa aaatattttg gaaagaatgt
     2581 tcaaactttg taactattaa tttattggtt tataatataa aaaaatttag agattaagaa
     2641 gcaggcgata tttaagctga gacgcgggtt aagcaagttg attattttat tcttggtctt
     2701 gtctgtggtt actgtaatta acttacttaa tttcataaag atagtttggg atcttgatct
     2761 gtattccgga atatgggcca ttaagatctg tccactggtc tccaacagta ccgataattt
     2821 cataaccttg ctgtgtaagt tctgtacgtt ttttactttt aaattcttgt gacttcaaat
     2881 ttagctcttc ttctctttga gtaattaatg tgtcaaactt tgtatatcca gcctgaataa
     2941 gatttttata agtaacctcg tactctgtat aatttctacc tgtcaggaat ataattttta
     3001 tacctttttt aagaagatac tcatacaagt cttttacttg aggaatagca ggcgctttta
     3061 attcttgatt ccatttttta ttgaggtcgt acacatatcc aaaatccatt tgcttggcaa
     3121 gaccgtaatt gtttagtgcg gt
//
LOCUS       DASWBY010000306         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_649322, whole genome shotgun sequence.
ACCESSION   DASWBY010000306 DASWBY010000000
VERSION     DASWBY010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649322"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1468)
                     /locus_tag="VF870_05725"
     CDS             complement(224..1468)
                     /locus_tag="VF870_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9251719.1"
                     /translation="MVEIINKACGLDIHKRFSIATILSRSGEKKQQRFSRDADGISNF
                     KNWVISEQCDVVACESTSDFWVPIYDSLIKHLPVIVGNARDMKAFTHKKTDKLDSEVI
                     AQLALNKMIQPSRVFAKNQREFRSYVRLRLTLVEKRTDLKNEAHSILASEMLHLKGVL
                     TDIFGKNGRVILSGISSGKNVDQIMENLSPAVHKKHDQIRAILDKEISQSSAFRLQIC
                     LKLIKSLDDEIEALEKEIFNYAYSNFSHEMDILMSVPGIGELSAATLIAEIGDFKDFS
                     SGDKLASWLGIVPNVYQSADKYHNGRITKRGSRVARWILTQIANAAARKKNSKLKEFF
                     NRKKKSIGHPKAIIALARKIATIIWHLVTIDEMYEDKTGYEKGEIQKRKIVQTEIFSI
                     DERIKIISGIIVIMGKEEEEST"
BASE COUNT          533 a          329 c          260 g          615 t
ORIGIN      
        1 aattttttaa ttttaaccaa aatttcaaaa tcaactaaca ttttaataat aactaacatt
       61 ttaatagtag ctaagatttt taaattaact gactttttaa attggctaaa atttcaaatt
      121 aactaagttc taaaactgac tcatctgtat tcggctaaaa tacgatctat aaaaaatgtg
      181 ttatttctgt tgatttagca tgaaagtact gaggatatct tcctcacgta ctctcttcct
      241 cctcttttcc cataattacg attattccac taattatttt gatacgttca tcaatggaga
      301 atatctcagt ctgaacaatc ttcctctttt gaatttctcc tttttcatat ccagttttat
      361 cttcgtacat ctcatcaatc gtaacaagat gccagataat tgtggcaatt ttccttgcca
      421 gggcaataat tgcctttgga tgtccaattg acttcttttt tcggttaaaa aactctttca
      481 acttactatt cttctttctt gctgctgcat tagcaatctg tgttagaatc cacctagcta
      541 ctcttgatcc tctctttgtg attctaccgt tatggtattt atctgctgat tggtacacat
      601 tcggaactat tccaagccat gaggcaagct tatctccaga agagaaatcc ttgaaatcgc
      661 caatttcagc aattaaagtt gccgcactaa gttcaccaat acctggaact gacattaaaa
      721 tgtccatttc atggctaaag ttactatagg cataattgaa aatctctttc tccaaagctt
      781 cgatttcatc gtcaagactc tttatgagtt taagacagat ctgaagccga aatgcagaac
      841 tttgagagat ttctttatcc agaatcgctc gtatctggtc gtgtttttta tgaacggctg
      901 gagaaagatt ttccataatt tggtcaacat ttttacctga agagattcca gataagataa
      961 ctctaccatt cttaccaaaa atatctgtca acacgccttt tagatgtaac atttcagatg
     1021 caagaatgga gtgagcttca ttttttagat ctgttctttt ttctacaagt gttaaccgaa
     1081 gtcgaacgta tgaacgaaat tctctctgat ttttcgcaaa aactcttgat ggctgaatca
     1141 ttttattcaa tgcaagttga gcaatgactt ctgaatcaag cttatctgtc tttttatgtg
     1201 taaatgcttt catgtctcgt gcattaccaa caataacagg tagatgtttt atcaacgaat
     1261 cataaatagg aacccaaaaa tcactggttg actcacaagc aacaacatca cattgttctg
     1321 atataaccca atttttaaag tttgaaattc catctgcatc tcttgagaaa cgttgttgtt
     1381 tcttttcacc agatctacta agaatagtag caattgaaaa acgcttatga atatccaaac
     1441 cacaagcttt gtttattatc tctaccaatt aaaatcacca aatttatttg ggcagtggaa
     1501 ttacctaaaa ggacaattta gcattcgtga tcgaagtcac agttgagcct tcgatggcaa
     1561 ttcactgatt agttttctga cggttttgat aaaccaaaat taggacaacc ttactgccca
     1621 aagtaatgag gaattcaata gcaaaaaagg tgcggtaaag tactttcatt ttgttgtgca
     1681 tgttattcga agaatatcat gtgcgttttc tatgaagctt gtgaagtttg tttcttt
//
LOCUS       DASWBY010000307         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_312786, whole genome shotgun sequence.
ACCESSION   DASWBY010000307 DASWBY010000000
VERSION     DASWBY010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312786"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VF870_05730"
     CDS             <1..805
                     /locus_tag="VF870_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9251720.1"
                     /translation="VLTVQSEIASNETQILIHEKQREAIVYYLNKLLGRDLNSKEVFA
                     LKDFEIDSLQLSQLQLEELLSDSNPTLNKMNNMIEMNNAMITANNKELIPDLMVQGML
                     MRMPRGMILTSASDLTMLDPKTEWMYSLMFSINLPFAPWSIGKFNNKEEELAAGISGI
                     EQEKNDMQREMTSQLKEALVKYNTAKELTDLYNKKVIPLYNQSTESQTTAYQNGQTNI
                     TAVIDSYRMLLMQKMNYYMSKADIQMSLAEVEMMVGANLKNLKLSEGNY"
     gene            807..>1830
                     /locus_tag="VF870_05735"
     CDS             807..>1830
                     /locus_tag="VF870_05735"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9251721.1"
                     /translation="MKDMKIKLMVSLLATLLFAFACSNSDDSNKNEKEYWTCTMHPQV
                     HKDGPGACPICGMDLIKKVVDDTADVSEKSDMEGMITLTTNKQILANVSTIKIKKENL
                     KKELTAYSYLDFVEQNRKTIPAKFNGRIEKLFVDKTGDYIKKGQPLFEIYSPDLVQAQ
                     NDYLVALSNNKNEINPLLDASKKKLELFGLTADQIDELKKSGEIKLTLTYYSPVSGTV
                     IEKKVQEGMYVNEGTVIYEVAELSTLWNIAEVNETDLSTIKLGSKVKLKLKAYPGEEF
                     MGKVTFIYPVINSQTRTVKIRSEFSSQNNKLKPQMYGETIFFDDAGTGLLVPADAVIF
                     SGNKNVV"
BASE COUNT          702 a          270 c          338 g          520 t
ORIGIN      
        1 tgttttaact gtacaaagtg agatagcctc aaatgaaact cagattttaa ttcatgagaa
       61 acaaagagaa gctatagtct attatctaaa taaactatta ggaagggatc taaattccaa
      121 agaagttttt gctttaaagg attttgaaat cgattcatta caactatccc aactgcaatt
      181 ggaagaatta ctttctgatt caaatccaac gttaaacaaa atgaacaata tgattgaaat
      241 gaataatgca atgataacgg ctaacaataa ggagttgatt ccggatttaa tggttcaggg
      301 aatgctgatg agaatgccca gaggaatgat tcttacatct gcgtcggatt taacaatgct
      361 tgatcccaaa accgagtgga tgtattcatt aatgttttct ataaatcttc cgtttgcacc
      421 ctggtcgatt ggaaaattca ataataaaga ggaagagctc gcagcgggta taagcggtat
      481 tgagcaagaa aaaaatgata tgcagagaga aatgacttca caacttaaag aagcacttgt
      541 aaaatataat actgctaaag aacttactga cttatataat aagaaagtaa ttccacttta
      601 taatcagtca actgaatctc aaacaacagc atatcaaaat ggacagacta acattacggc
      661 agtaattgat tcttatagaa tgctgcttat gcaaaagatg aattattata tgtcaaaagc
      721 tgatattcag atgtcgcttg cagaagttga aatgatggtc ggtgctaatc ttaaaaatct
      781 aaaattatct gaaggaaatt attaaaatga aagatatgaa aataaaatta atggtcagtt
      841 tattagcaac attattgttt gcatttgcct gctcaaattc ggatgattca aataaaaacg
      901 aaaaagaata ctggacatgc acaatgcatc ctcaggttca taaagatgga cctggtgcat
      961 gccctatttg cggaatggat ttgattaaaa aagttgttga tgatacagcg gatgtttctg
     1021 aaaaatcaga tatggaaggg atgataacct taaccaccaa taaacaaatt ctggcaaatg
     1081 tatctacaat taagatcaaa aaagaaaatc taaaaaaaga attgactgca tatagttatc
     1141 ttgattttgt tgaacagaat cgcaaaacta ttccggcaaa attcaacggg agaatcgaaa
     1201 aattatttgt tgataaaacc ggtgactaca taaaaaaagg tcagccgttg tttgaaattt
     1261 atagtcctga tctcgtgcag gcgcaaaatg attatttagt tgcattgagt aataacaaga
     1321 acgaaataaa tccattgctt gacgcatcaa agaaaaaact tgaattgttt ggtttaacag
     1381 cagatcaaat tgatgaacta aaaaaatcag gtgaaataaa attaacatta acatactact
     1441 cccctgtcag cggaacggtt attgaaaaga aggtacagga aggaatgtat gttaacgaag
     1501 gaactgtgat ttacgaagtg gctgaacttt caacactctg gaatattgca gaagttaatg
     1561 agactgatct ttctacaata aagttgggaa gtaaagtgaa actaaaacta aaagcatacc
     1621 ctggtgaaga gtttatgggt aaagttacat tcatctatcc cgtaattaat tcacaaacca
     1681 gaactgtgaa aatccgaagt gagttctcaa gccaaaacaa caaacttaaa ccacagatgt
     1741 atggtgaaac tattttcttt gatgatgctg gaacaggatt attagtccct gccgatgctg
     1801 taattttttc aggaaataaa aatgttgtgt
//
LOCUS       DASWBY010000308         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_126601, whole genome shotgun sequence.
ACCESSION   DASWBY010000308 DASWBY010000000
VERSION     DASWBY010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126601"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VF870_05740"
     CDS             complement(<1..677)
                     /locus_tag="VF870_05740"
                     /inference="COORDINATES: protein motif:HMM:NF027594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HEX9251722.1"
                     /translation="MVMFSEDELILKLVDKNNYSDAAKRLFELQSKEWPMLNAGFKSL
                     ETVRSKSFQFDGYKIKAQFNAGRMTSTSAKVDPKSISERECFLCAENLPIEQKGILYN
                     NEYIILCNPFPIFPTHFTLTHKGHQPQRIIDTFSDMLDLSKNLSKHYTVIYNGPRCGA
                     SAPDHLHFQAGNKFFMPIDDEFHKIKNEYGKKIFEDDDLSFYAIDDKVRKFISIESID
                     KKIIINT"
     gene            complement(683..1798)
                     /locus_tag="VF870_05745"
     CDS             complement(683..1798)
                     /locus_tag="VF870_05745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9251723.1"
                     /translation="MKILYSCLSKSWGGMEMITLIFIRKLLERNCQVELLCIEESRMQ
                     IEANNLGLIIHPVKASGYVHPGAILKIASLIKNNNYDLLHTQASKDLWLVVPALKIAS
                     SQIPLFLTKQMGSFIVKKDFMHKFLYQRVTKAFAISTVIKNNLLETTPLSEDKIELLY
                     NAVDVKKFNPVKADRNKVRKEFNISDKEIVIGMIARFSRGKGHEEFLQSAKALSSKFN
                     NLKFTIVGEASRGEDEYANTIKGLAEKLGLRNIIFAGFRSDTPDVLSAFDIFIFPSHA
                     EAFGIALIEAMAMERASVCSNSDGVLDIAVDGETNLLFENKNAEDLAHKMEELIIDED
                     KRIRMGKEARRRVLEKFDIEIITDRTIEFYNNFINKN"
     gene            complement(1832..>2267)
                     /locus_tag="VF870_05750"
     CDS             complement(1832..>2267)
                     /locus_tag="VF870_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9251724.1"
                     /translation="DYDQETKDFYRWKVEYTQAQISELIMRKSGIDFGLIKDLIPIER
                     GFSSRLVKLRIVGTKKTITIGKELEIRRILSETHLYSSAIVVEKSGAVNGVPEKFVLY
                     GAGWGHGAGLCQIGAAVMAEQGYQFDEIAIHYFKDAKLKKIY"
BASE COUNT          703 a          443 c          328 g          793 t
ORIGIN      
        1 aatgtgttta taattatttt tttatcaatg ctttcgattg aaataaattt tctcacttta
       61 tcatcaattg cataaaatga taaatcatca tcctcaaata tttttttgcc gtattcattt
      121 tttatcttat ggaattcatc atcaatgggc ataaagaatt tattgcctgc ctgaaaatgc
      181 agatgatcgg gggcagaggc tccacatctt ggaccgttat aaataactgt gtaatgttta
      241 gaaagatttt tactcaaatc aagcatatcg gaaaaagtat caataattct ctgcggttga
      301 tggcctttgt gagttaacgt aaagtgagtt ggaaatatcg ggaaaggatt acataatatt
      361 atgtattcat tattgtataa aattcctttt tgttctatgg gcagattttc tgcacacaaa
      421 aaacattctc tctcgcttat agatttagga tcaacctttg cggatgtaga agtcattcgt
      481 cccgcattaa actgtgcttt aattttatac ccatcaaatt gaaatgactt tgatcggacg
      541 gtttcaagtg atttgaatcc agcattaagc atcggccatt ctttgctctg cagttcaaac
      601 aaccgcttgg ctgcatcaga ataattattt ttatcaacaa gttttagaat taattcatct
      661 tctgaaaaca ttaccatagc tctcaatttt tatttataaa attattataa aactcaattg
      721 ttctgtcagt tataatttca atatcaaatt tttcaagcac tcgcctcctt gcttctttac
      781 ccattctaat ccttttatct tcatcaataa tcaactcttc cattttgtga gcaagatctt
      841 cggcattttt attttcgaat aacaaatttg tttcgccatc aacagcaatg tcaagcactc
      901 catcagaatt agaacaaact gatgctctct ccattgccat tgcttcgatc aatgcaatac
      961 caaaagcttc tgcgtgcgat ggaaatataa aaatatcaaa agcagaaagc acatcggggg
     1021 tatcactgcg aaatcctgca aagataatat ttctgagacc gagtttttcc gccaaacctt
     1081 ttatggtatt agcatattca tcttcaccac gacttgcttc cccaacgatc gtaaatttaa
     1141 gattattaaa ttttgatgaa agtgcttttg ctgattgcaa aaattcttcg tgacctttac
     1201 cgcgactaaa tcttgcaatc attccaatta caatttcctt atcactgata ttaaactctt
     1261 ttcgtacttt attccgatct gcttttaccg gattaaattt tttaacatcc acagcgttgt
     1321 aaagtaattc aattttatcc tctgacagcg gtgttgtttc aagcaaatta tttttaatta
     1381 ctgtgctgat cgcaaaagct ttggttacgc gttgataaag aaatttgtgc ataaaatctt
     1441 ttttaacaat aaatgatccc atctgcttgg ttaagaataa tggaatttga gatgaggcaa
     1501 ttttcaaagc gggtacaaca agccaaagat cttttgaagc ctgtgtgtgt aagagatcat
     1561 aattattatt ttttataagc gaagcaattt tcaaaatagc acccggatga acataaccag
     1621 aagctttaac gggatgaata attaatccaa gattatttgc ttcaatctgc attctggatt
     1681 cctcaataca taaaagttca acctggcaat ttctttctaa tagcttacga atgaaaatta
     1741 acgtaatcat ttccattccg ccccagcttt tagataaaca ggaataaagg attttcattt
     1801 aatcgtactc acgctcttaa cctcacactt ttcagtaaat ctttttaagc ttagcatctt
     1861 taaagtaatg aatagcaatc tcatcaaact gataaccttg ctctgccatt actgcggcac
     1921 ctatctgaca aagtccggca ccatgtcccc atcctgcacc gtacaataca aatttttcag
     1981 gaacaccatt tacagctccg gacttttcaa caacaattgc agagctgtaa agatgagttt
     2041 cagacaatat tcttctaatc tcaagttctt taccgattgt tattgttttt ttagtaccta
     2101 ctattctaag ttttaccagg cgcgaagaga atcctctttc aatcggaata agatctttaa
     2161 tcaatccaaa atcaattcca cttttacgca tgataagttc ggatatttgt gcttgtgtat
     2221 attcaacctt ccatctgtaa aaatcttttg tctcctgatc ataatcc
//
LOCUS       DASWBY010000309         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_48045, whole genome shotgun sequence.
ACCESSION   DASWBY010000309 DASWBY010000000
VERSION     DASWBY010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48045"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..>2632)
                     /locus_tag="VF870_05755"
     CDS             complement(161..>2632)
                     /locus_tag="VF870_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX9251725.1"
                     /translation="DYDLFADDILFSPQFPLLNDNVNISASIKNIGKNSLAFSLDLYE
                     DTNLDSIPDLLIETLSNLNLAAVDSAVYQFNYTIQNIQDKKAFYIKALFFQDQDTMNN
                     SVYKTIEPGYPNQTIVVNEIMFAPLGGEPEWIELFNNSDAEINLKDWAIWDVVTTPVK
                     VAIKSDLTIPANSYVVLTKDSLIINYHRFISSEILELSLPSFNNDRDGVVLKDNRGIT
                     IDSVFYTNQWGGTNGFSLERISTTNSSNNQFNWLSSIDVEQSTPGRINSVTPKEYDLS
                     VAEISFSPRFPTTGDNVSISAKIKNNGNQSAQNFITEFYIDTDSNNIADLLLSSINSS
                     DLNPGDSILITSSLQLQDLQNKILTAVRVVYESDEDTLNNYFEKSIEPGFAQNIVKIN
                     EVMYNPADGEPEWVEFINVSSDSINLKNWFISDVLTTPTKNFITNDDGFVQANEIFIV
                     AKDTSFSSAHPDSKAKVFYSNFGSLGNTFDGIVIYDFRNGIIDSLFYQSGWGGSKGFS
                     LERISLDVQNNDSTNWVTSLDESGSTPGLINSINNVPAYNRNDLIINEIMYDPETNNS
                     EYVEFYNPSPDPVNIGGWKFEDENGNINKLTETSFIIQPQEYFILIADSSALVYYNLF
                     NYNKKNIIGSSSLGLVNTGELILLKDVRGNVIDSVFYDSKWNNRNIASTKNKSLERIN
                     PSLNGNDPLNWSTSVNSLGGTPGMQNSIFAENLNQSANISVYPNPFSPDNDGFEDFTV
                     INYNLSQATAQVRIKIFDSKGRLVRSLLNNQASGSSGSVIFDGLDDENNALRIGIYII
                     FLEALNDNSGIVETIKSTVVVARKL"
BASE COUNT          883 a          431 c          405 g          913 t
ORIGIN      
        1 gaaatgaatg atataaaaat tgaaagaacc agaatagtat ttgttgttaa gcgcatttta
       61 attcgttaaa tattattgat ctaaaaatac gaatttgatt tgtgacctgc agggtttatc
      121 aacagaataa ttaaagcagc aaaaaaaact cactctaatc ttaaagtttg cgggcaacaa
      181 caactgtgga ttttatggtt tcaacaatac ctgagttatc attaagtgcc tctaagaaaa
      241 tgatatatat tcctatccgc aaagcattgt tttcatcatc aagtccatca aatattacag
      301 agccgcttga accacttgct tgattgttta ataatgaacg aactaatctg cctttgctgt
      361 caaatatttt tattcgtact tgtgcggtgg cttgtgataa attataattt ataactgtaa
      421 aatcttcaaa gccatcatta tccggtgaaa atggatttgg ataaaccgaa atattagctg
      481 attgatttaa attttctgca aagatactat tctgcattcc cggtgttcca ccaagtgaat
      541 tgacagaagt actccagttt aatggatcat taccatttaa gcttggatta attctttcta
      601 aagatttatt ttttgttgaa gcgatgtttc tattattcca tttactatcg tagaagacag
      661 aatcaattac atttcccctt acatctttta ataaaattaa ttctccagtg tttacaagtc
      721 caaggcttga tgatccaatt atattttttt tgttataatt aaaaagattg taataaacca
      781 atgcagagga atcagcaatc aaaatgaaat attcttgtgg ttgaattata aagctagttt
      841 cagttaactt attaatgttt ccattctcat cttcaaactt ccatccgcca atgtttacgg
      901 gatccggact ggggttatag aactcgacat actcactatt gttagtttcg ggatcataca
      961 taatttcatt aattattaaa tcatttcgat tatatgcggg aacgttatta attgagttta
     1021 ttaaaccggg agtgcttcca ctttcatcaa gtgaagtaac ccaatttgtg ctgtcattat
     1081 tttgaacatc taaagaaatt ctttccaaag aaaagccctt acttccgccc cagcctgatt
     1141 gataaaacaa gctatcaata atgccgtttc taaaatcata aataacaata ccatcgaatg
     1201 tatttcctaa gcttccaaag tttgaataga agactttagc tttgctatca ggatgcgcgg
     1261 aactaaatga tgtatctttt gctacaataa atatttcatt agcctgcacg aaaccatcat
     1321 cattcgtaat aaaattttta gttggagttg taagcacatc actaataaac cagtttttaa
     1381 gattgattga atctgaactt acattaataa attccaccca ttccggttca ccatcggctg
     1441 gattgtacat cacttcattt atcttaacta tgttttgtgc aaaacccggt tcaatggatt
     1501 tttcaaaata gttattcaaa gtatcttcat ctgattcata gacaactctt acagcagtta
     1561 aaattttatt ttgcagatcc tgtaattgta atgatgaagt tattaaaata gaatcacctg
     1621 gatttaggtc cgatgaattg atagaactta aaagtaaatc ggcaatatta tttgagtctg
     1681 tatcaatata aaattctgta atgaaatttt gcgctgattg gttcccatta tttttaattt
     1741 ttgcagagat tgaaacatta tctcccgtag ttgggaatct tggtgaaaaa gaaatttctg
     1801 caacggataa atcatattct tttggagtta cactattaat tcttcccggt gtactttgtt
     1861 caacatcaat actgcttagc caattaaatt gattgtttga agaattggtg gttgaaatcc
     1921 gttcaagtga aaatccattt gtaccgcccc attgatttgt ataaaaaaca gagtcaattg
     1981 taattccgcg attatctttt agtacaactc catcccgatc attattaaaa gaaggaagag
     2041 agagttccaa aatttcagat gaaataaatc tgtgataatt aattattaaa gaatctttag
     2101 ttaaaacaac ataagagtta gcaggaattg ttaaatcact ttttatagcg actttaacag
     2161 gagttgtcac aacatcccaa attgcccaat cctttaaatt gatttcagca tccgagttgt
     2221 tgaacaattc aatccattcg ggttcacctc caagaggtgc aaacattatt tcatttacta
     2281 caattgtttg gtttggatat cccggttcaa tggttttata cacggaatta ttcattgtat
     2341 cttgatcctg aaaaaataat gcttttatat aaaacgcttt cttatcctga atattttgga
     2401 tagtataatt gaactgataa actgcagaat caaccgctgc aagattaaga ttagaaagtg
     2461 tttcaattaa taaatccgga atagaatcaa gatttgtgtc ttcgtaaaga tcaagagaaa
     2521 atgctagtga gttcttccca atgtttttta tggacgcaga aatattaaca ttatcattta
     2581 gcaaaggaaa ttgcgggcta aataaaatgt catctgcaaa taaatcataa tc
//
LOCUS       DASWBY010000310         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_86619, whole genome shotgun sequence.
ACCESSION   DASWBY010000310 DASWBY010000000
VERSION     DASWBY010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86619"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VF870_05760"
     CDS             complement(<1..356)
                     /locus_tag="VF870_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9251726.1"
                     /translation="MKNTLKIFLILLAVISTRCLDDSGVPAEDIVLDDTAKILRYIES
                     TGDFANTDLAPALVSASELFNNRAQYFILDIRTPEEYSAGHIEFAVNISSANLYQVVD
                     SLYTNFPFKKIVVVSK"
     gene            complement(362..1162)
                     /locus_tag="VF870_05765"
     CDS             complement(362..1162)
                     /locus_tag="VF870_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251727.1"
                     /translation="MKHLKIISLLLVLFFAGCENEELLNPEISYQEKLVVQCQFYADI
                     NFPGVIITKTLPLEVEFDIKLAEIKDATLYLRVNGFQIIPLHYISDGLYKPLYDFRPK
                     EGDYYELFGEWNEYNFYALTKVPLKPVINNVNFNSTGYFAEAGVTAFKDEVYSALWAV
                     DLGHYKTADDFYNISVPPANSTNYTINVRSAAYPVEYQSAVYNGRRYMQIYSFDSSFD
                     AYFKTKVQNEEINNPYVQGSGNTVWNVKGENVIGMFIGLNKSNFIFIN"
     gene            complement(1165..3492)
                     /locus_tag="VF870_05770"
     CDS             complement(1165..3492)
                     /locus_tag="VF870_05770"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9251728.1"
                     /translation="MRSANLSIISIVILLGFFQSNLSQEKNISISGFIYDAVTGEALI
                     GTNILVYKDTINFNVGPVYGAAANNYGFYMLPKLNREKYYLIFRYIGYKAAIKEMDLT
                     FSESNVNYNVKLNPENIKLDEIVIEGKKIDKNVLSTVDVSPELLTKLPSFTGEVDLFR
                     SLRLVPGVNKSSELSNGLYVRGGTPDQTLTLVDGAILYNPSHIGNIASTFNSNAIRDI
                     KLIKGAFPAEYGGRLSSVLDIKLRSGTKEKETGTVGLGTINSFFSFEGPLKESATYMI
                     AGRWMYYDAWQRSFDKQSSVPRYNFYDINGKINYNVSDASAFSLSIMFSRDNMYNPPA
                     DDIIYDINWENLNVTLNWVQINSKSLFLNSSLSYVNYEFSTKIGVGNETTSSASYFSN
                     PNLTDLILRQNAEVNWEIDQKIKTGFEVSFHNYDLLYNEYYDLSYEQDPYAGDNINSI
                     EAALFFQNESEFFNSLKTNIGGRLIYFNANGQLSFEPRISLSYLMFSDFYIKGAAAVA
                     NQYLHLITRNDIALPTDLWYPATANIKPSRSTQFVLGFDKYFDQDSYQTSVEGYYRNM
                     KNLYEFKNSPEINPLNDSIEDQFVQGEGEAYGVEVFFQKRKGNLQGWIGYTLSWTKRK
                     FDDLNLGKVFYPKYDRRHDISVVATYNLTNELTFGATFTYATGMRYNSPTGQFTFNPI
                     GVSGLPQTLLDYPGLNVSQFPAYHKLDLSFNYKIKMTKVEINLFANLYNIYNRKNAYA
                     QFIVFPANTDGDQVPQLKRISLFPFIPSAGVSIIF"
     gene            3694..>3798
                     /locus_tag="VF870_05775"
     CDS             3694..>3798
                     /locus_tag="VF870_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9251729.1"
                     /translation="MKKIRNIAIIAHVDHGKTTLVDQILKQCAVFRTNQ"
BASE COUNT         1270 a          677 c          588 g         1263 t
ORIGIN      
        1 tttttactaa ccacaacaat ttttttgaat gggaaatttg tgtaaagaga atcaacaact
       61 tgataaaggt tagctgatga aatatttact gcaaattcaa tatgtccggc agaatattct
      121 tccggagttc ttatatctaa aataaaatat tgtgccctgt tattaaacaa ttccgaagca
      181 gatactaatg ccggagccag atcggtattt gcaaaatctc ctgtactttc tatatatcgc
      241 aatatctttg cggtgtcatc caacacaata tcttcagcag gaacaccact atcatcaagg
      301 catcgtgtgg aaataacagc aagcagaatc aaaaatattt taagagtatt tttcataaac
      361 attaattaat aaaaataaaa tttgatttgt ttaatccgat aaacattcca attacatttt
      421 cacctttaac attccacaca gtatttccgg atccctgaac ataaggatta ttaatttctt
      481 cattttgtac ttttgtttta aaatatgcat caaacgaact atcaaaagaa tagatctgca
      541 tataccttct gccgttgtaa accgctgact gatattccac cggataagct gcacttcgaa
      601 cattgattgt ataattagtt gaatttgccg gaggaactga tatattataa aaatcatccg
      661 cagttttata atgaccaaga tcaactgccc agagcgctga gtaaacttca tctttaaatg
      721 cagttacacc ggcttcagca aaatacccag tgctattaaa atttacatta ttaataaccg
      781 gctttagagg aacttttgtc aaagcataaa aattatattc attccattca ccaaataatt
      841 cataataatc cccttctttc ggtctaaaat cataaagagg tttatacaaa ccatccgata
      901 tgtaatgaag cggaattatt tgaaatccat taactcttaa atataaagtt gcgtctttta
      961 tctcggctaa tttaatatca aactcgactt ccaatggcaa tgtttttgtg atgataaccc
     1021 cgggaaaatt gatatcagca taaaactggc attgaactac taatttttcc tgatagctta
     1081 tttcaggatt aagtagttct tcgttttcac aaccagcgaa gaataatact aacaacaacg
     1141 atataatttt taagtgtttc ataattaaaa tattatgctt actcccgcag aaggaataaa
     1201 tgggaatagt gaaattcttt ttaactgcgg tacctggtct ccatcagtat tagctggaaa
     1261 aacaataaac tgtgcatacg cgttttttct attataaata ttgtagagat tcgcgaataa
     1321 atttatctca acttttgtca tcttgatctt ataattaaaa cttaaatcca gtttatgata
     1381 agctggaaat tgtgaaacat taaggcccgg ataatccaat aacgtctggg gtaaacctga
     1441 aactcctata ggattaaagg taaattgtcc tgttggagaa ttatatctca tacctgttgc
     1501 atatgtaaaa gtagcaccaa aagtaagttc attggttaaa ttatacgttg caactacaga
     1561 aatatcatgc cgcctatcat atttaggata aaacacttta cctaagttta aatcatcaaa
     1621 ttttcgtttc gtccatgata atgtgtatcc gatccatccc tgcagattgc cttttctttt
     1681 ctggaaaaaa acttctactc cataagcctc accttcaccc tgcacaaatt gatcttcaat
     1741 tgaatcatta agtggattaa tctccggtga atttttaaac tcgtataagt ttttcatatt
     1801 ccgataataa ccttcaactg aagtttgata ggagtcttga tcaaaatatt tatcaaatcc
     1861 taatacgaac tgtgtagatc tgctcggttt aatattggca gttgcgggat accagagatc
     1921 cgtcggcagt gcaatatcat ttcttgtaat cagatgaaga tattgattag caactgccgc
     1981 tgcacctttt atataaaaat cgctgaacat taagtatgat aatgaaatcc tgggttcaaa
     2041 actaagttgc ccgttcgcgt taaaataaat taaccttccg ccgatatttg tttttagtga
     2101 attaaaaaat tcagattcat tttgaaaaaa taatgctgct tctattgagt taatattatc
     2161 gcctgcataa ggatcctgtt cgtagctcaa atcataatac tcgttataga gaagatcata
     2221 gttatgaaaa gaaacttcaa atcccgtttt tattttctga tcaatttccc aatttacttc
     2281 ggcattctga cgaaggatta aatctgtaag atttggattt gaaaagtacg atgctgaact
     2341 ggttgtttca tttccgacac caatcttagt tgaaaattcg tagttaacat agcttagtga
     2401 ggaatttaga aataatgatt tagagtttat ctgcacccag tttaacgtta catttaaatt
     2461 ctcccaattt atatcataaa taatatcatc agccggaggg ttgtacatat tatctcgact
     2521 aaacattatg cttaaagaaa aagcgctggc atcggaaaca ttataattta tttttccatt
     2581 aatatcataa aaattatatc gcggtactga actctgctta tcaaaacttc tttgccatgc
     2641 atcataatac atccatcggc ctgcgatcat ataggttgct gattctttaa gcggaccttc
     2701 aaatgaaaaa aaagaattta tggttcctaa tcctaccgtt ccggtttctt tttctttggt
     2761 gccggatcgc agtttaatat ctaacacact ggaaagcctt ccaccgtatt cagccggaaa
     2821 ggcaccttta attaatttta tatctcttat agcgttagaa ttaaaagtac tcgcaatatt
     2881 tccgatatgc gaaggattat ataaaattgc tccatcaacc agggtaagtg tttgatccgg
     2941 tgtgccgcct cttacatata aaccatttga tagttccgaa gatttgttaa cgcctgggac
     3001 aagccggagc gaacgaaata aatcgacttc accggtaaaa ctgggcagtt ttgttaagag
     3061 ttcaggagag acatctacgg tacttaaaac atttttgtca attttcttcc cctcaataac
     3121 tatttcatct aactttatgt tctctggatt tagcttaaca ttataattaa cattcgattc
     3181 tgaaaatgtt aaatccattt cttttatcgc ggctttatag ccgatgtacc ggaagattaa
     3241 ataatatttt tctctgttaa gttttggaag catataaaag ccataattat tagcagctgc
     3301 accatataca ggtcctacat taaaatttat tgtgtcttta tataccagga tatttgtgcc
     3361 aatcagtgcc tcaccggtta cagcatcata aataaaaccg gatattgata tatttttttc
     3421 ttgagataag ttagattgga aaaatcctaa aaggattaca atggagatta ttgatagatt
     3481 agcagagcgc ataatactct gaaaattatt attaaaacta tattagaaat agataaaata
     3541 tcataaagag tcaatagtta atttgattgt ccgctatcga taggttaagg atctttctta
     3601 caattctatc ggttgtgtaa aagcacaagt cattctgact tctgaccata cttgcgtaaa
     3661 ttagcacctc aaaatttaat cacttggaga acgatgaaaa aaattagaaa catagccata
     3721 attgcccacg ttgatcatgg taagactacg cttgtggatc agatattaaa acaatgtgct
     3781 gttttcagaa caaatcag
//
LOCUS       DASWBY010000311         8140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_13823, whole genome shotgun sequence.
ACCESSION   DASWBY010000311 DASWBY010000000
VERSION     DASWBY010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13823"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /gene="pruA"
                     /locus_tag="VF870_05780"
     CDS             <1..1358
                     /gene="pruA"
                     /locus_tag="VF870_05780"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX9251730.1"
                     /translation="KEWSELPWEHRASVFLKIADLLAGPWRSTINAATMLGQSKTVYQ
                     AEIDSAAELIDFYRFNSYYMAQLMQDQPYSGSGMWNRVEYRPLEGFIFSVTPFNFTSI
                     AGNLPTAPAIVGNVSLWKPASSAVYSAYWLMKLFEEAGLPKGVINFVPGSGGQVGTPA
                     FTNPNLAGVHFTGSTEVFQNMWKTISENLKNMKYYPRIVGETGGKDFIFVHNSADVDA
                     LVVAALRGAFEYQGQKCSAASRMYIPKSLWKEFKEKYVAEVGKIKMGDIEDFTNFMGA
                     VIDKGAFKSITDHIKYAKDSKEAEIITGGNFDDSKGYFIEPTTIVTTNPKFKTMEEEI
                     FGPVMTIYVYDDNKLDETLNLCDETSPYALTGAIFAQDRNVIVKLSNRLRNAAGNFYI
                     NDKPTGAVVGQQPFGGGRASGTNDKAGSAMNIMRLMTARTIKETFDPPKDWRYQFMSE
                     K"
     gene            1504..2721
                     /gene="sucC"
                     /locus_tag="VF870_05785"
     CDS             1504..2721
                     /gene="sucC"
                     /locus_tag="VF870_05785"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX9251731.1"
                     /translation="MKIHEYQAKETLKKFGVPVQDGIAIKSMLEFDGAITQLQQRGIN
                     QFVVKSQIHAGGRGKGIVYDVRNRDVVILEGGVKFTTSPEKAAEYAEKILGNVLVTHQ
                     SGPEGKVVKTLFITEGLDYKKELYLGILLDRAVSKNVIMVSTEGGVEIEKVAEETPEK
                     IIKEWIEPGVGLQSYQARKLAFALGLEGSAFKSFIPFIKALYKAYEATDASMLEINPL
                     VITNDDRVVALDAKMNFDDNALYRHPEILDFRDLDEEDPLEIEASKYNLNYIKLDGNV
                     GCMVNGAGLAMGTMDIIKLAGGEPANFLDVGGGANKETVANGFKIILSDPNVKAILIN
                     IFGGIVRCDRVAQGVIDAATEMQVHVPIVVRLEGTNAKEAAELLDQSSLHFEVAKSLQ
                     DAAEKVTAVLRVN"
     gene            2984..4402
                     /locus_tag="VF870_05790"
     CDS             2984..4402
                     /locus_tag="VF870_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9251732.1"
                     /translation="MSFFDSEFSNEEEYDLPEENVEEELASCKKILDSGYVFDSVERI
                     EELIQVCMDIDRYEDALFLLDRFLEIFPYNSEYWLKKGIVLNGLFRFSEALLAYEQSL
                     SLNPNDTETLIDKSATEENLGLYDHSQESLEKVLELDPENDDAMFSLGLLFQRRAQLK
                     TAIEYFQKVVARDPDYTEVYYELGFCYENSAQLDKALESYDKFLELDPYNPNGWYNRG
                     IVLVKMGKLEQGINSYDLALSIRENFSSAWFNKGNALADLGRLHEAIICFKKSFELDK
                     DDETAVYNIANIYEELDDLPSAIKYYTESIKLNEEYYDAYLSRGFCYDSMGKYQLALR
                     DFNKAISLSQDSAEAWYAKADLEYSLGQIHDSIFSYREAARIEPDNYEVWFNLAETYF
                     ESGEWLEALKAFDECIRINPKDATSFYGKAKANFVLSRTQEAIECLKSAFVFDPSIRT
                     EFEKEYPDIKSSKLFKRLIGEL"
     gene            4433..5305
                     /gene="aroE"
                     /locus_tag="VF870_05795"
     CDS             4433..5305
                     /gene="aroE"
                     /locus_tag="VF870_05795"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX9251733.1"
                     /translation="MQNSFYANTELIGLIGYPIKQSFSPFIHNVAAELTGTRIIYLPF
                     EVHSSNLKNAVKGIVSLGIRGFNVTVPHKVKVLDYLNKLSEEAAVIGSVNTVVNEMGK
                     LIGYNTDVNGIVESLSPFKTQINESEVCVIGAGGSARAVIYTLIRNFKPSKIILINRT
                     EQFAETLKQHFKSKMRFDGIVTKELQQPDLKSLLNCCSLIVNCTSVGMYPASDDSILT
                     TSDVFVKDQIVFDLIYNPVKTKFLQLAESKGAVTINGLNMLVQQAAKSFTLWTGKEFP
                     TDKVYKSLLLYVTK"
     gene            complement(5307..6497)
                     /locus_tag="VF870_05800"
     CDS             complement(5307..6497)
                     /locus_tag="VF870_05800"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9251734.1"
                     /translation="MLADRVKEIGASPTMMVAAEAKKLKSEGVDVIDLSMGEPDFHTP
                     NSIKAAGINAIEENRTRYTLNQGTNELRTAISAKLKRDNNLDYNLNELIVSSGAKQSV
                     YNSILAIVNPGDEVIIPAPYWVSYPAMVMLAGGKNVVINTNEKMGFKITPEQLQSVIT
                     NKTKMLILCNPSNPTGSAYTKSELEALAEIVLKHNLYVLSDEIYEKLVYDNFEFVSFA
                     ALSPDMKKKTILVNGISKSYAMTGWRIGYTAASENIVSAINKIQSHTTSHASSISQYA
                     AIEAVAGPQYVISEMLEEFKKRREYFYNELTSIKGITCYKPEGAFYLFPSISNYLHTK
                     SDVLKIENSFDFAMHLLYDAHIATVPGSAFGSEGHIRMAYATSMDHLHEAIFRLKTTL
                     SKIH"
     gene            complement(6614..7114)
                     /locus_tag="VF870_05805"
     CDS             complement(6614..7114)
                     /locus_tag="VF870_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251735.1"
                     /translation="MIKYFIPLFFGFVLIVSCAIKDEKLQHLKEYIQKIAEEIKYEDG
                     FNTIKVLGEQRSVFRSYFLNDALVFINEEISIGNRGSSLNHYYFKDDQLVSYEQKSIL
                     MKDDSLNIKAKTMITFSMLLDNRIVLESDYWIGSAQTTVVESDVEKVIKHAELLKELA
                     VKNRPK"
     gene            7252..8097
                     /locus_tag="VF870_05810"
     CDS             7252..8097
                     /locus_tag="VF870_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9251736.1"
                     /translation="MVKLLCSVLFVFGFQLISFSQDKSCCSTGKDVSFASFSEEKEFR
                     DIHQVPAAFILKDAKGKMITFNTKDGKAANGYFVKSEKKSDKFIFVFHEWWGLNDYIK
                     KESDELQQKLGDVNILAIDLYDGNVANNREDAAKYMQSVNQERALNIISGAIDFAGKN
                     AKIGTLGWCFGGGWSLQASIMLGKQGSACVMYYGIIENTPETFKNLNAPVLGIFAEKD
                     AWVNPEVYGNLEKNIKAAGKNITVKSFNADHAFANPSNEKFAEKATKEAKELTLKFFK
                     DNLIK"
BASE COUNT         2737 a         1274 c         1523 g         2606 t
ORIGIN      
        1 gtaaagaatg gtcagaattg ccctgggaac atagagcttc tgttttctta aagatcgctg
       61 atttgttggc aggaccctgg cgttcaacaa taaatgcagc tacaatgctc ggacaatcta
      121 aaacagttta ccaggcagaa atagattccg ctgctgaact aattgatttc taccgattta
      181 acagttatta catggctcag ttgatgcaag atcaaccata ttccggtagt ggaatgtgga
      241 atcgtgtaga gtaccgacct ctggaaggtt ttattttttc tgttacacct tttaacttca
      301 catctattgc aggtaacctt ccaactgcac cggcaattgt cggtaatgta agtttgtgga
      361 aaccagcatc gagtgctgtt tactccgcat actggttaat gaaattattt gaagaagcag
      421 gattaccaaa aggagtaata aattttgttc ccggttccgg tggacaggtt ggaactccgg
      481 catttacaaa tccaaatctt gcaggtgttc attttacagg atcaacagaa gtgtttcaaa
      541 atatgtggaa aactatatca gaaaatctta agaatatgaa atactatcct cgaattgttg
      601 gtgaaactgg cggtaaggat tttatttttg ttcataactc agcagatgtt gatgctctcg
      661 ttgttgcagc attacgtgga gcatttgagt atcaggggca aaaatgttct gcagcatcaa
      721 gaatgtatat tccaaaatct ctatggaaag agtttaaaga aaaatatgtt gcggaagtcg
      781 gcaaaattaa aatgggtgat attgaagact ttacaaactt tatgggtgct gtaattgaca
      841 aaggtgcatt caaatccata accgatcata ttaagtatgc aaaagattct aaagaagcag
      901 agattataac aggcggaaat tttgatgatt caaaaggtta ttttatcgag ccaacaacca
      961 ttgtaaccac aaatccaaaa ttcaaaacaa tggaagaaga aatttttgga ccggttatga
     1021 caatttatgt ttacgatgat aacaaacttg atgaaacttt aaatctgtgt gatgagactt
     1081 ctccgtatgc acttacaggt gctatctttg cacaggatag aaatgtaatt gttaagttat
     1141 caaacagact tagaaatgca gcaggtaact tttacataaa tgataaacca actggagcgg
     1201 tagttggtca gcagccattt ggtggaggaa gagcatccgg tacaaatgat aaagctggaa
     1261 gtgcgatgaa cataatgaga ttgatgacgg caagaacaat taaagaaact ttcgatcctc
     1321 ctaaagattg gcgttatcag tttatgagtg agaaataagg cgtattcaga aacattttta
     1381 actgtcatcc cgagcggagt cgagggagga aggatgacaa attcttatac aatgcgaggt
     1441 cacacttcga cttcgctcag tgtgacataa aagtttatta aaaaaataat tcaaggaagt
     1501 taaatgaaaa tacacgagta tcaggcaaaa gaaacattaa agaaatttgg tgttcctgtt
     1561 caggatggaa ttgcaataaa aagcatgtta gaatttgatg gtgcaataac acaacttcaa
     1621 cagcgcggta taaatcagtt tgtcgttaaa tcacaaatcc atgccggcgg aagaggtaaa
     1681 ggaattgttt acgatgtaag aaacagagat gtagtaattc ttgaaggggg agttaagttt
     1741 acaacttctc ctgaaaaagc tgctgagtat gctgagaaaa ttcttggcaa cgttttagta
     1801 actcatcaat ctggtcccga aggcaaagtg gtaaaaactt tatttattac agaaggattg
     1861 gattacaaaa aagaattata tcttggaatt cttttagatc gtgcagtttc taaaaacgtt
     1921 attatggttt ctacagaagg cggcgttgaa attgaaaaag ttgctgaaga aactcctgaa
     1981 aaaattatta aagaatggat tgaaccaggt gttggactgc aatcttatca ggctcgtaaa
     2041 ttagcatttg cactcggact tgaaggcagt gctttcaaga gtttcatccc ttttataaaa
     2101 gcactatata aggcctatga agcaaccgat gcgtccatgc tggaaataaa tccattagtt
     2161 attacaaatg atgatcgtgt tgttgcactt gatgcaaaaa tgaattttga tgacaacgct
     2221 ctttatcgtc atcctgaaat tcttgacttc agggatttgg atgaagaaga tccattagaa
     2281 attgaagctt caaaatacaa tcttaattac atcaaacttg atggtaatgt tggctgtatg
     2341 gttaatggtg caggactggc aatgggaacg atggatatta ttaaacttgc aggcggtgaa
     2401 ccggctaact tcttagatgt cggcggcgga gcaaataaag aaacagtagc aaatggattc
     2461 aaaataattt tatcagatcc gaatgttaaa gcaattttaa taaacatctt tggcggaatt
     2521 gtaagatgcg atagagttgc tcaaggtgtt attgatgcag caacagaaat gcaggttcat
     2581 gttccgatcg tcgtaagact tgaaggaaca aatgcaaaag aagctgctga attacttgat
     2641 caatcgagct tacactttga agttgcgaag agtttgcagg acgccgctga aaaagttaca
     2701 gcggttttaa gagttaatta aataatttag ttcatagctc agactttagt ctgagcttac
     2761 ttctattata aagatttgaa ctttattaaa atttataatt tacgtttttc aaatttctct
     2821 ttttaggcgg aataacaaaa agggaaattc agatcaataa aaaagttgaa tttaatattt
     2881 tcaatacaaa atttttaagt aactaacaaa taatcctcac cttgattttc tattttatta
     2941 gttatagatt tttcctgaaa caattttcag ttaggagaac ttaatgtctt ttttcgattc
     3001 cgaattttct aatgaagagg aatacgatct gcccgaagaa aatgtagaag aagaattagc
     3061 ttcgtgcaaa aaaattctgg atagcggtta tgtatttgat tctgttgagc gaatagaaga
     3121 gcttatacaa gtatgcatgg atattgatcg atatgaagat gctttatttt tattagatag
     3181 gtttttagaa atctttcctt ataactcgga gtactggtta aagaagggaa tagttcttaa
     3241 tggcttgttc cgtttcagtg aagctctatt agcttacgag caatctctct cacttaatcc
     3301 aaatgataca gaaactctga ttgataaatc agcaacagaa gaaaaccttg ggttatacga
     3361 tcactcacaa gaatccctgg aaaaagtttt agaactggat cccgaaaatg atgatgcaat
     3421 gttcagtctt ggattacttt ttcagagaag agcacaactt aaaacggcaa ttgaatactt
     3481 tcaaaaagtt gttgcaaggg atcctgatta tacagaggtt tattatgaac tgggtttctg
     3541 ttatgaaaac tcagcacaac tcgacaaagc attggaatca tatgataaat ttcttgagct
     3601 tgatccttac aatccaaatg gatggtataa caggggaata gttctcgtta aaatgggcaa
     3661 acttgaacaa gggattaaca gttatgatct ggcgctttca atccgtgaaa atttttccag
     3721 tgcctggttt aataaaggta atgcacttgc agatctcggt cgcttgcatg aagcaatcat
     3781 ttgttttaaa aaatcttttg aactggacaa agatgatgag accgcagttt acaatattgc
     3841 aaatatttat gaagagcttg atgatttacc ttcggcaata aaatattata ctgagtcaat
     3901 taaactaaat gaggaatatt atgatgctta cctttcacgc ggattctgtt acgattccat
     3961 gggtaaatat caattagcct taagagattt taataaagca atttcacttt cacaggatag
     4021 cgcagaagca tggtatgcaa aagctgatct tgaatattcg cttggacaaa ttcacgatag
     4081 tatttttagt tacagagaag cggcgaggat agaacctgat aattatgaag tatggttcaa
     4141 tcttgctgaa acttattttg aatcaggcga atggctggaa gctctaaaag cttttgatga
     4201 atgcatcaga ataaatccca aagatgcaac atcattttat ggtaaagcca aagcaaattt
     4261 tgttttaagc agaactcagg aagcaattga atgtttaaaa tcagctttcg tttttgatcc
     4321 ttctatcaga acagaatttg aaaaagagta tcccgatata aaatcatcaa aactttttaa
     4381 aagacttatc ggtgaacttt gatttattct aataattatt aaattttatt ttatgcaaaa
     4441 ctcattttat gcaaatacag aattaattgg attgattggt tacccgatta aacaaagttt
     4501 ttctcctttt attcacaacg ttgcagcaga gttaaccgga accagaatta tttatcttcc
     4561 ttttgaagtt cattcaagta atttaaagaa tgcggttaaa ggaattgttt ctcttgggat
     4621 acgaggattt aatgttaccg tgccgcataa agtaaaagta ttagattact tgaataaatt
     4681 gtccgaagaa gcagccgtta tcggttcggt taatactgtt gtaaatgaaa tgggcaagct
     4741 aattgggtat aacactgatg ttaatggtat tgttgagtca ctatcgccgt ttaaaacgca
     4801 aataaatgaa agtgaagttt gtgttattgg cgccggcgga tctgcacgtg ccgtaatcta
     4861 tactttaata agaaatttta aaccatcaaa aataattctg attaatagaa ctgaacagtt
     4921 tgcagaaaca ttgaaacagc actttaaatc taagatgaga tttgatggaa ttgtaacgaa
     4981 ggaattgcag cagccggatt taaaaagttt attaaactgc tgttcgctta tcgttaattg
     5041 tacttctgtt ggaatgtatc ctgcttcaga cgacagtatt ttaactactt cagatgtatt
     5101 cgttaaagat cagattgttt ttgatttgat ctacaacccc gttaagacaa agtttttaca
     5161 acttgccgaa tcaaaaggcg cagttactat aaatggattg aatatgcttg ttcagcaggc
     5221 ggcaaaatcc tttacgcttt ggacaggaaa agaatttccg accgataaag tttataaatc
     5281 acttctgcta tacgtaacta agtaaattaa tgaattttac ttaacgtcgt ttttaatcta
     5341 aaaatagctt catgtaaatg atccattgaa gtagcgtaag ccatcctgat atgaccttca
     5401 cttccaaaag cgcttcccgg cacagtggca atatgagcat cataaagcaa gtgcattgca
     5461 aaatcaaatg agttttcaat tttcagtaca tcagattttg tgtgaaggta attagaaata
     5521 cttggaaata aatagaaagc tccttcgggt ttatagcagg ttattccttt gattgaagtc
     5581 aattcattat aaaaatattc ccgtcgtttt ttaaactctt cgagcatttc gctaataaca
     5641 tattgcggac cggcaacggc ttctattgct gcatattggg aaatcgatga agcgtgtgaa
     5701 gttgtgtggc tttgaatttt attgattgca cttacaatat tttcagaagc agcagtgtaa
     5761 ccaattctcc atcccgtcat ggcataagat tttgaaatac catttacaag gattgttttc
     5821 tttttcatat ccgggctaag agccgcaaag cttacaaact caaaattatc atagactaac
     5881 ttttcgtaaa tctcatcaga taaaacatac aaattgtgtt ttaaaacaat ttctgctaag
     5941 gcttccagtt ctgattttgt ataagcagat ccggttggat ttgatgggtt acaaagaatc
     6001 agcatttttg ttttattagt tattacagac tgcaactgtt ccggagttat tttaaacccc
     6061 atcttctcat ttgtattaat cacaacattt tttccaccgg caagcataac catcgcggga
     6121 tatgaaaccc agtatggtgc aggaataatt acttcatcac ccggatttac aattgccaga
     6181 atgctgttgt aaacactttg tttggcaccg cttgaaacta taagttcatt taaattgtaa
     6241 tctaaattat tgtcacgttt taatttagct gaaattgcag ttcgtaattc atttgtaccc
     6301 tgattaagag tgtaccgcgt tcgattttct tcaattgcat ttatgccggc tgcttttata
     6361 ctatttggag tgtgaaaatc aggctcaccc atacttaaat caattacatc cacaccttca
     6421 gactttaatt ttttggcttc cgctgcaacc atcattgttg gtgatgcacc aatctcttta
     6481 actctatcgg ctaacaatta tatctcctgt aactttaaaa ttttgagaaa agtttgtaaa
     6541 tcagatataa acaaataatg cgagtttatt ttctaacaag tacgactata actttaagaa
     6601 acaaacatta tttttatttc ggcctgttct ttactgcaag ttctttaagt aattctgcat
     6661 gtttaattac tttttcaaca tcactttcaa ctactgttgt ttgcgcactg ccaatccaat
     6721 aatcagattc aagtactatt ctattatcta gtaacattga aaatgtaatc atagttttag
     6781 ctttgatatt taaagagtca tctttcatca aaatcgattt ttgctcgtaa ctaacaagct
     6841 gatcgtcttt aaagtaataa tgattcaaag aagacccacg attaccaatg cttatctcct
     6901 cattaataaa aaccaatgca tcattcaaaa aatatgaacg aaaaacagat ctttgttcgc
     6961 ccagaacttt aattgtatta aaaccatctt catattttat ttcttcggct attttttgaa
     7021 tatattcctt taaatgctga agtttttcat ccttgattgc acaggataca attaaaacaa
     7081 atccgaaaaa cagaggtata aaatatttga tcataacaat taaaagttta tttataaatg
     7141 atgtttaatc taaacccaaa atgttttttt tacaatgaat atttcgatgg aacttactat
     7201 cttattcatg agtttatatg tttgttatat caataagaaa aaggagaaaa aatggtaaaa
     7261 ttactttgtt ctgtcctgtt tgtctttgga tttcagctga tttctttttc gcaggataag
     7321 tcttgctgct caaccgggaa ggatgtttca tttgcatctt tctcagaaga aaaagaattt
     7381 agagatattc atcaagttcc tgctgctttt attttaaagg atgcgaaagg taaaatgata
     7441 acctttaata caaaagatgg caaagctgca aatggatatt ttgtaaaatc agaaaaaaaa
     7501 tctgataaat ttatttttgt atttcatgaa tggtggggat tgaatgatta tatcaagaaa
     7561 gaatctgatg aattgcaaca aaaacttggt gacgtaaata ttttagctat tgatttatat
     7621 gacggaaatg ttgctaataa cagagaagat gccgcaaagt atatgcaatc tgttaatcag
     7681 gaacgagcat taaatattat ttcaggagcc atagattttg cgggtaaaaa tgcaaaaatc
     7741 ggaactctag gctggtgttt tggcggtgga tggtctttac aggcaagtat tatgttaggc
     7801 aaacagggaa gcgcttgtgt tatgtattat ggaattattg agaacactcc ggaaacattc
     7861 aaaaatttaa atgcacctgt gctaggaata tttgctgaaa aagatgcctg ggttaatccg
     7921 gaagtttatg gaaatcttga aaagaatatt aaagcagcgg gcaaaaatat aaccgtaaaa
     7981 agttttaatg cggatcatgc ttttgcaaat ccgagcaatg aaaagtttgc tgagaaagcg
     8041 actaaagagg ctaaagaact caccttaaaa ttctttaaag ataatttaat taaataaata
     8101 aaaaagccat ccaatttcgg atggcttttt atatcactct
//
LOCUS       DASWBY010000312         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_13939, whole genome shotgun sequence.
ACCESSION   DASWBY010000312 DASWBY010000000
VERSION     DASWBY010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13939"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VF870_05815"
     CDS             <1..447
                     /locus_tag="VF870_05815"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX9251737.1"
                     /translation="GVIGKSNGEMSWHVKEEFQHFKNTTLGFPIIMGRKTFETLGKPL
                     KGRLNIVVSKNKSYKTSYDDVVIKSSIENAIEYCKPLKTEKIFIIGGGEIYRQAILFV
                     DEMMITFMKFVAEGEVKFPKINNDDWTIEKIKDHELFEIFRYVRKS"
     gene            447..>1698
                     /gene="mutL"
                     /locus_tag="VF870_05820"
     CDS             447..>1698
                     /gene="mutL"
                     /locus_tag="VF870_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX9251738.1"
                     /translation="MASRIKILPDNLASKIAAGEVVQRPESVVKELLENAVDANATSV
                     ELIVKQAGKSLIQVCDNGTGMTEEEALLCIHKHATSKISSMEDLESIKTLGFRGEALS
                     SIASVCQLEIRTETRESEIGTLLKVESENEIITEKISVPKGTCVAVKNIFYNIPARRK
                     FLKTDNTELKHIIDTFNRIALAHPEISFKFYNAENLVFDYKIGKLEERIQQVFADNMM
                     EALIPVNQQTDYLSINGYIGKPSIFKKSRGEQYLFLNKRFVVNKNINHAVFTAFENIL
                     EKGDYPFFILFVTLDPRKVDVNIHPSKLEVKFDDEKDIYNFVLSVVRKSIGSYDLVPT
                     MSFDDATPQSEKLSFNAFTSVKQNDFSDRPNFNERERRERTAVTDEDIDLVFSSLTKD
                     IKPSSSSSLNSSAGKGYEQTENKFD"
BASE COUNT          607 a          238 c          330 g          523 t
ORIGIN      
        1 ggagtgatag gtaaatccaa tggagaaatg tcctggcatg taaaggaaga atttcaacat
       61 tttaaaaata caactctcgg ctttccaata attatgggca gaaaaacttt tgaaacatta
      121 ggcaagccgc taaaaggcag attgaatatt gttgtaagta aaaataaatc atataaaact
      181 tcttatgatg atgttgttat caaatcctca attgagaatg ccattgaata ttgtaaaccc
      241 cttaagacgg aaaaaatatt tatcattggc ggcggtgaaa tatacaggca ggcaattttg
      301 tttgttgatg aaatgatgat tacatttatg aaatttgtag ctgaggggga agttaaattt
      361 cccaaaataa ataatgatga ttggaccatt gagaaaataa aagatcacga attgtttgaa
      421 atatttagat atgtaagaaa gagttaatgg cctcacgaat aaaaatatta cccgataatc
      481 ttgcaagtaa aattgctgcc ggcgaagttg tgcagagacc tgaatctgtt gttaaggaac
      541 tcttggaaaa tgcggttgat gctaatgcga cttctgttga attgattgta aagcaagctg
      601 gtaagtctct aattcaagtt tgtgataacg gcacaggaat gacagaagag gaagctctgc
      661 tgtgtattca taagcacgca acaagcaaaa tatcttctat ggaagatctt gagtcaatta
      721 aaactctagg ttttcgcggg gaggctttaa gttctatcgc ttcagtttgc caacttgaaa
      781 tcaggacgga gacacgtgaa agtgaaatcg gaactttgct aaaagttgaa tctgaaaatg
      841 aaattataac tgaaaaaata tctgtaccaa aagggacctg tgttgccgta aaaaatattt
      901 tttataacat cccggcaaga agaaaatttc tgaaaactga taacacagaa ttaaaacata
      961 ttattgatac gtttaataga attgcgctag ctcatcctga aatatcattt aaattttaca
     1021 atgcagaaaa tcttgtcttt gattataaaa tcggtaagct tgaagaacgc attcaacagg
     1081 tttttgctga taatatgatg gaagcattaa ttcctgtaaa tcagcaaaca gattatcttt
     1141 caattaatgg atatatcggc aagccttcta tttttaagaa aagcagaggc gagcagtact
     1201 tatttcttaa taagcgattc gttgtaaata aaaatatcaa tcacgctgta tttacagcgt
     1261 tcgaaaatat tttggagaaa ggtgattatc cattctttat tttgtttgta acattagatc
     1321 caagaaaggt tgatgttaat attcatcctt caaagcttga agttaagttt gatgacgaaa
     1381 aagatattta caattttgtt ttatctgtgg ttcgtaaaag cattggcagc tatgatttag
     1441 tcccaacaat gtcttttgat gatgcaactc cgcagagcga aaaactttct ttcaatgcat
     1501 ttacttctgt gaagcaaaat gatttttctg atagacctaa ttttaatgag cgggaaagac
     1561 gggaacgtac tgcagtaact gatgaagata ttgatctggt ttttagttcg ttaacaaaag
     1621 atataaaacc gagttcaagt tcaagtttaa attcaagcgc agggaaaggt tatgaacaaa
     1681 ctgaaaataa atttgata
//
LOCUS       DASWBY010000313        18303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_725251, whole genome shotgun sequence.
ACCESSION   DASWBY010000313 DASWBY010000000
VERSION     DASWBY010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 18303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18303
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725251"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..557)
                     /gene="trxA"
                     /locus_tag="VF870_05825"
     CDS             complement(195..557)
                     /gene="trxA"
                     /locus_tag="VF870_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9251739.1"
                     /translation="MTEALTKETFLTKVFDYEKNQDWKFSGELPAIIDFWAPWCGPCR
                     MVGPVLEELSEEYKGKLNIYKVNTDEEQELGAVFGIRSIPSILFVPMTGQPKMAVGAL
                     PKESIKEAINKELLIAVS"
     gene            complement(691..1074)
                     /locus_tag="VF870_05830"
     CDS             complement(691..1074)
                     /locus_tag="VF870_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX9251740.1"
                     /translation="MKYGFSKTTDYGFEQAIEKVTEELKKEGFGILTTIDVKDTLKKK
                     IDVDFKKYTILGACNPKLAHGALQVEEELGLLLPCNVIVYEKNDKTVVSIFDPIVMTI
                     VIENPEMKSVAEEVKGKLKRVLEAI"
     gene            complement(1074..1310)
                     /locus_tag="VF870_05835"
     CDS             complement(1074..1310)
                     /locus_tag="VF870_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251741.1"
                     /translation="MERIRFEIIWKWAKRILPVVAGAGAGYSYYYFVGCNGSCPISGN
                     PYISTAYGAFAGFLFVDWKSIFNKNKKEVTKENE"
     gene            complement(1286..1675)
                     /locus_tag="VF870_05840"
     CDS             complement(1286..1675)
                     /locus_tag="VF870_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9251742.1"
                     /translation="MSTEQIILYVLIALIIYYIVKKIYLIKSIKQYTPADASAKVKKE
                     RNVVFLDVRTEKERKQSSIKGSFHIPVTSIKDKEQELKKFKDAEIICYCQTGNRSLTA
                     ASKLKKMGFNASNLSGGMVRWNVSGLR"
     gene            complement(1824..3086)
                     /locus_tag="VF870_05845"
     CDS             complement(1824..3086)
                     /locus_tag="VF870_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9251743.1"
                     /translation="MQKLKSGFQHSFWVANGMELFERLAYYGQATILSVFLRDHLKFT
                     EVEAGQLSSIFGGLIYFLPIFAGALADKFGFKKAFSFAFFVLAIGYFLIGSTGIAAFS
                     GWYTDVNLFLVLSVILIFTAIGGSFIKPSVLGTVALTTTHETKSLGYAIYYWLVNIGA
                     AIGPLLAYFVRDSFGIEFVYLVSAISCALMFFTTLFIFKEPEAKTVEKRDDIVTVAKN
                     LVTVVKNIKFMFFLMLFALYWIVFWEFFIVIPFYVSDYISPEAPIELILSTGAWTIIL
                     LQIPINRFTKNIPTPTAIMIGFVFAALSWFLLYFSQIAGITVGIVLIISTIFIFSVGE
                     QTQAPRFYEYLADLAPKGQAALFQGFAFLPIAIAWLVGGTFGGWLYQTFATTAKQPEM
                     VFLVIGLVGVAGAILMWIYNKFLNVKEV"
     gene            3442..3927
                     /locus_tag="VF870_05850"
     CDS             3442..3927
                     /locus_tag="VF870_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251744.1"
                     /translation="MKTFLITFLLILLNTINFSQNKKAVISEIKNDEVKVKLHRFIEF
                     DDSKAKSGNKFLVADISLENISSKSIAMGSDYTMTITIKDDKGNEYRSGLKGEGIVST
                     YLIRQDSEEQDTKAHNLCFSDNFPAKTKARSLLCGFEVPKDAKIVSFGLKKKNLWSAI
                     K"
     gene            4179..5510
                     /gene="rsxC"
                     /locus_tag="VF870_05855"
     CDS             4179..5510
                     /gene="rsxC"
                     /locus_tag="VF870_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEX9251745.1"
                     /translation="MKSRTFHGGVHPKEYKELTEHSAFEFMPYPEEIILPLSQHLGKE
                     ASPQVKKGDEVTPGQLVAKAEGFISAPVHTSVAGKVMSLGKEQTSSGFPKDSIVIKTN
                     GTVESEKFLFPPLNPDTITADEIRERVALAGIVGQGGAAFPTSVKLTLPKDKVIDVVI
                     LNGCECEPYLTRDYRFMIERPDDLISGLKLIMKALNVNRGVIGIEDNKPEAIELLSNK
                     VKFEPGLEVIALKTKYPQGAEKMLIKAVTGREVPPGKLPMDVGAVIQNIGTALAIHDA
                     IIKGEVLITAALTVSGKGIKNPKNLIVSVGTPIQNVIDFCGGVTEDAVKVVVGGPMMG
                     VAQFDLHAPVMKATSGILVLTKDEVAENPETPCLRCGQCVGACPLNLMPTKLARYSQL
                     NRFEDAEGAGITVCMECGTCAYTCPANIPLVQWIRLGKQKVLQMQKERMGK"
     gene            5556..6581
                     /locus_tag="VF870_05860"
     CDS             5556..6581
                     /locus_tag="VF870_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HEX9251746.1"
                     /translation="MLKRVQHDYYKEIMETTTTTEPKQKVYFDLITSPHLHSKWSTSK
                     VMWFVVAALVPSIAAALTFFGWTQIFIIITAVVFAVGTEASIQAIRKKQITWFDGSAA
                     LTGLLLALTLPPNFSLSATAIGSVIAIGLGKQIFGGLGYNIFNPALVGRAFLQAAFPV
                     QITTWTKPNFAVDSVTSATPLAALKFDKIFADTSSLAIGNIGGSLGETSAIAVLIGGI
                     LLISVGIVNWRIPLSMIIGMFVFGGIFWLINPQNPTPVFHIFAGSFLFGAMFMATDWV
                     TSPITNKGMWVFGLGISLVLVVIRIYGGLPEGVMYSILFMNGFVPLINRYTRPGIFGA
                     VKEGKKK"
     gene            6578..7141
                     /locus_tag="VF870_05865"
     CDS             6578..7141
                     /locus_tag="VF870_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX9251747.1"
                     /translation="MKVTHIVAALTLIGIVAGGSLSLVNNWAAPKIALNQKAETERAI
                     FLVHLDGKRYDEIKNTGFEVYKVFDEKDSSVGYSLVYAGNGFQGKIKLMIGLTEDLNK
                     ITSIEVLEQSETPGLGTKILEPPYKDQYNGLIPVPSIKLVKGVEPSNPNEVKAITGAT
                     ISSRSVVAIVNEGVSKLKELESKGALK"
     gene            7138..7830
                     /locus_tag="VF870_05870"
     CDS             7138..7830
                     /locus_tag="VF870_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HEX9251748.1"
                     /translation="MKKQVTLLREFTKGLWEINPTFKQILGMCPTLAVTVSAINGIAM
                     ALATTFVLFFSSLIISLIRKWIPDQVRIASYIVVIATFVTIVDLVMKAQFPDLSKALG
                     PFIPLIVVNCIILGRAEAFASKNNPLRSMLDALGNGVGFLISLFALGSIREIIGSRTI
                     LGYQVMPNGFEPWLIMILPAGAFLTLGLMMGFANLYVERKKNLEREALIAQYKRVGRE
                     EITDEVLKEAGV"
     gene            7832..8404
                     /gene="rsxA"
                     /locus_tag="VF870_05875"
     CDS             7832..8404
                     /gene="rsxA"
                     /locus_tag="VF870_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEX9251749.1"
                     /translation="MELLIIFISAALVNNFVLSYFLGICPFIGVSNKLSSAISMGMAT
                     TFVMALTAIVSWLLYNLILVPYHLDFLQIPSFILVIAALVQFVEMVIKKVSQPLYRAL
                     GIFLPLITTNCAILGLALLASMRQYNFMESVIFGVGAGAGFTLALIIMAGIREELDLA
                     DVPKPFQGAPITLIVAGILALAFMGFAGMI"
     gene            8510..9244
                     /locus_tag="VF870_05880"
     CDS             8510..9244
                     /locus_tag="VF870_05880"
                     /inference="COORDINATES: protein motif:HMM:NF018685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEX9251750.1"
                     /translation="MISRRNFLKIAGLSSVALGAGFTAGKLVGNKKSVYFAVHGFIPA
                     DEQVIANLVTAFKNKTKCNSNPVIVSKTKIGETINRFDLNARKENFKNSGSVTYRIQR
                     LDKQIDSDLIVSDANNSVYSLNDLNSSFENIRSGLKNRKADLLFTAEYKETDFLSTLL
                     NSDRKEVVIENEKGLVDRISLNNTYKNILIGGPQGKTGLKIENGTAQVHTSTCRHGIC
                     KHSVASGPGNIIACAPNKVLVKIERS"
     gene            9246..10235
                     /locus_tag="VF870_05885"
     CDS             9246..10235
                     /locus_tag="VF870_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX9251751.1"
                     /translation="MKNKIEYGVLVVVLFLIGFFIAKNSSDELKTFKRTQILLGTVVE
                     IQVRDVDEKKADDAISKAFAEVKRIDDLFTTFNDRSPVWQINSSVDTIIDVEPEIYNL
                     IVLCDSITKLSDGGFDISLYNLTKAWGFYTDNPHLPSKGAIDSALLVSGWQKINLLDK
                     NRIEKKEKVELNFGAIAKGYAVDRAIEVLKRSGITQALVNAGGEISVLGNDWIVGIQH
                     PREINSIIKKIKLDEYTVATSGDYEQYFEVDCVRYHHILDPKTGYPSRGLQSVTIINK
                     SNTFADGLATTIFVMGEEKGMQLVESLNDTEAMIIDSAGKIFYSSGFEKFLVN"
     gene            10345..11199
                     /locus_tag="VF870_05890"
     CDS             10345..11199
                     /locus_tag="VF870_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEX9251752.1"
                     /translation="MDITLIIAIATMGGLGFIFAGALAFADKKLRVEEDPKIAQVNDV
                     LPNANCGACGNAGCYDFAVKVVNGEAKISGCPVGGQEVVDEIAKILGMESTQSVKLVA
                     RILCNGGNIEAVKKEGIEYLGPQSCSVKTIVAGGDKMCLYGCLGGGDCVDACQFGAIF
                     MNENGLPTVVEELCTGCGQCVDACPRGVIEIHPEGRELFIFCKNHDDPKRSKEVCNVA
                     CFGCGICARKSDGSIEMRDFLPEIDYTKLDLSKIPIDKCKTGAIRLIHPERTTELLKE
                     ENIEVKVS"
     gene            11286..11726
                     /locus_tag="VF870_05895"
     CDS             11286..11726
                     /locus_tag="VF870_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HEX9251753.1"
                     /translation="MYKEELYEDGIVKESQNGFATIIISDSDHCIECSAKLYCKPGNS
                     NERSLTAADSYGAHPGDKVRVSISGRKILSAAFLLYGIPLILLMAGLLFGSKIFSYHQ
                     ELFSTLLAIMLISIYLIGLFFFSKHNKHNVSFHPQITFISSSTV"
     gene            11830..12381
                     /locus_tag="VF870_05900"
     CDS             11830..12381
                     /locus_tag="VF870_05900"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9251754.1"
                     /translation="MKKFQVFLFVLFITSGAAIFSIAYGEQPVFVKEFIGQMDYVQGK
                     ILQLEDAMPQDKFSWRPEEGVRSVGEVNLHMAFSNYLFIKTPGGTPPPDINMDFNKWD
                     YQTNDKKEIAAIIKRSFDDAKKYASGLTENDLNKEVDFFGNKMTVRNFMVTMLNHLHE
                     HLGQSIAYARMNKITPPWSKKGE"
     gene            complement(12440..14320)
                     /gene="pepF"
                     /locus_tag="VF870_05905"
     CDS             complement(12440..14320)
                     /gene="pepF"
                     /locus_tag="VF870_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX9251755.1"
                     /translation="MVKYVLLLLVSFLTAEGAYAQKVYENRDEIPSEYKWNLNDIYPD
                     WNDWENDLTNLKTKMDEIISYKGKLKENPENLLKVMQLNDQLGILSYKIYRYPQLHMD
                     LDTRNQQMGANLQKVQAMFAEFGVATSWINPELLEIPWDTMNKWLNSNPAFEPYRFGI
                     ETLYRQQMHVLSADKEELLSYFSRLRSSPSDIYSNLTTADVDFPTVVLSNGDSVQATY
                     GNYGRILATDKNQEDRKKIFEAHYGVFKKNENTHASIYSAVCQADWASARARGYNSSL
                     EAELDGNNIPVEVYENLVNTVKANTEPLQKYNRLRKKLLGLDKYYSFDGSIPLTDFTK
                     TYPYEEAKEWVLASVSPLGAEYKEKLSGALKGGWIDVFENKGKRAGAYSAGVYGVHPY
                     MLMNYNETLDNVFTLGHELGHTMHTLYSQENQPFATSSYTIFVAEVASTFNERLLLDY
                     MLSQTTDPKERINLLQQAIQGVTGTFYFQTLLADYELKVHTLVEKGQSVTSETLTNIM
                     SDLYDKYYGDSIEKDEFINHVWARIPHMFRTPFYVYQYATCFASSAQIYNNIFKGSES
                     EKKEAMDKYLTLLKSGGNDYPMEQLKKAGVDLTKPETIKAVISQFSELVDKLEAEINK
                     LN"
     gene            complement(14471..15403)
                     /locus_tag="VF870_05910"
     CDS             complement(14471..15403)
                     /locus_tag="VF870_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9251756.1"
                     /translation="MIEKNKIEYNTKLFPSLLVIIAASLWAVDGIILRPSLYSLPVPL
                     VVLVESAIVAVLLTPFFINKFSSLKHLTKKDWLAFFGVALLGGAVGTMAITKALFYVN
                     FVNLSVVILLQKLQPVFAILLATIFLKEKLPKEFFLWAGLAIVGAYFMTFGLSLPNFS
                     TGDKTTIAALFALLAAFSFSSSTVLSKRALRNVDYEMGTYLRFLFAAIIMLIIASTTG
                     DISSITEITTKQIIIFLIIAFTTGGAAIFLYYYGLKHISASVASICELAFPLTAIILE
                     YFVHGNILSPVQWIGAVLLLVSILMVSGIRTITE"
     gene            complement(15412..15684)
                     /locus_tag="VF870_05915"
     CDS             complement(15412..15684)
                     /locus_tag="VF870_05915"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HEX9251757.1"
                     /translation="MESYLGYLAAFCTTIAFIPQAIKVYKSKHTKDISLGMFSLLNAG
                     FVLWLWYGILILSYPIIIANAITIVIAVYIFITKLRLDVFSSDKSN"
     gene            16002..17150
                     /locus_tag="VF870_05920"
     CDS             16002..17150
                     /locus_tag="VF870_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251758.1"
                     /translation="MQDQLTKETISGKFTEDFDLDIEKTNNKNIVPAELPNWNKAETS
                     LQNKLQFVLRRVNLSKSFLDDDVSKWVNASNEPYELSALRTYLLLSCVNMIGEICRTD
                     TPMTFLSWLDTSNSVTTRSIREESISKAINSVGVIDDQKKAVKLIKSVFAQYKLTNSV
                     DSNYFSFFTDALDVETQNWIASNSWIYQDNPLEKWANLQNVKEGYANLTSAENKRLTN
                     SRKRWDGLDINQKLFEVSQFLEFLFNQYNLCQVNSPVKMDKDPVAKVWKDIDQAVSEY
                     KVNYLSNSLFNQMGADGQKFKFNEVRCVFKEEELIVFEGDVREKDRELWLKRLSEWVK
                     TKIESESAIGETIFLAPKNQALVLSGENLAALLEKWIDRAVKNILNKK"
     gene            complement(17225..17398)
                     /locus_tag="VF870_05925"
     CDS             complement(17225..17398)
                     /locus_tag="VF870_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251759.1"
                     /translation="MKKKKSKKNLQENPNQLTLEFLYKEPPIDVHIPKSATTVFNTHF
                     TTTKRLAIRLAES"
     gene            complement(17664..18029)
                     /locus_tag="VF870_05930"
     CDS             complement(17664..18029)
                     /locus_tag="VF870_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251760.1"
                     /translation="MKKFLVFVLLVIAGYFAYDNFIKEKEVYEIKDSYNKARASVDLD
                     APALSPTDYAHYQGTIKNISDETLNNIVIEYLIDAQPSTAKIDKLEPGEEKNFTTNSV
                     MLRNMDPAHYLKSVNYDRK"
BASE COUNT         6095 a         2988 c         3317 g         5903 t
ORIGIN      
        1 ctctttgatt aaagagcttc ggtgattatg gtagaaaact tttacttgtt gataaaattt
       61 tatgtgattt tttactttaa gattttgatt tacttattca atattataaa tcttaatgtc
      121 taaaaatctt taaaagaaaa aacgcatccc atttccggaa tgcgttaatt cattggggct
      181 tatgaaaaaa tattttagga cacagcaatc aatagttctt tattaattgc ttcctttatt
      241 gattcttttg gaagggcacc aactgccatc tttggctgtc ctgtcatagg cacaaaaagt
      301 attgaaggaa tacttctaat tccaaataca gcacctaatt cttgctcttc atctgtgtta
      361 actttataaa tatttaattt acctttatac tcttcagata attcttcgag aaccggacca
      421 accattctgc atggaccgca ccagggggcc cagaaatcta taattgctgg aagttcgccg
      481 ctgaatttcc aatcttgatt tttttcgtaa tcaaaaactt ttgttaaaaa agtttcttta
      541 gttaatgctt ctgtcatttg tttgcctttc ttatgattct tttattttct taataattat
      601 agcgccagtt ttcgacttct ctaaatgtga tgttattttg ctttcgcttt cgtagtcatc
      661 ctgagcgaag tcgaaggatg aggattattt ttatattgct tccaacactc ttttcaactt
      721 gcctttaact tcttctgcaa cagatttcat ctccggattt tcaattacta ttgtcataac
      781 tatcgggtca aaaatggaaa caacggtctt atcattcttt tcataaacga ttacattaca
      841 aggtaaaagt aaacctaact cttcttcaac ctgtaaagct ccgtgtgcaa gttttggatt
      901 gcaggcacca agtattgtgt acttcttaaa atcaacatct atctttttct ttaaagtatc
      961 tttaacatca atagttgtta aaataccaaa gccttctttc ttaagttctt cagttacttt
     1021 ttcgattgct tgttcaaaac cataatcggt tgtttttgaa aaaccatatt tcattattcg
     1081 ttttcctttg ttacttcttt tttattttta ttaaatattg atttccaatc aacaaaaaga
     1141 aatcctgcaa atgcgccata cgctgtactg atatatggat tacctgaaat agggcagctt
     1201 ccattgcatc ccacaaaata gtaatatgaa tatcctgcgc ctgctccagc aacaacggga
     1261 agaattcgct tagcccattt ccatattatc tcaaacctga tacgttccaa cgaaccattc
     1321 ctccacttaa attacttgca ttaaaaccca ttttttttag tttagatgca gcggtcagac
     1381 ttcggtttcc ggtctgacaa tagcaaatta tttcagcatc tttgaacttt tttaattcct
     1441 gttctttgtc tttaatcgaa gttacgggaa tatgaaatga accttttata gaactttgtt
     1501 ttctttcttt ttcagtgcga acatccaaaa aaacaacatt tctttccttc tttacttttg
     1561 ccgaagcatc agctggagtg tattgtttta ttgattttat taaataaatc tttttcacga
     1621 tataataaat gataagggca attaaaacgt ataaaatgat ttgttctgtt gacataattt
     1681 ttcttttttg tgattgattt gatatgcaaa ttaagaaaag gattcagatt ttgttgtgat
     1741 ataaactttg gagttgtttt gtgtttttgg ggatttagtg gcaaaaaaat catgccacta
     1801 aaactccaaa attctctgcc aaattaaact tcttttacat ttaaaaattt attgtagatc
     1861 cacattaaaa tagcaccagc aacaccgact aacccaatta caagaaaaac catctcgggc
     1921 tgctttgctg ttgtagcaaa tgtttgataa agccaaccac caaaagtacc gccaaccagc
     1981 caggcaattg caatcggaag aaatgcgaac ccctgaaaca atgcagcctg tcctttgggt
     2041 gcaagatcgg caagatattc ataaaatctt ggtgcttgtg tttgctcgcc aacagaaaaa
     2101 ataaatattg ttgaaattat taatacaata ccaacagtaa ttcctgcgat ttgtgaaaag
     2161 tataaaagaa accagcttaa tgctgcaaat acaaatccta tcataattgc tgtgggtgtg
     2221 ggaatgtttt ttgtaaatct attaattggg atttgtaaaa gaataattgt ccatgcgcct
     2281 gtagaaagaa ttaattcaat cggggcttca ggtgaaatgt agtctgaaac ataaaatggg
     2341 atgactataa aaaactccca gaacactatc cagtacaacg caaataacat aagaaagaac
     2401 ataaacttta tattctttac aaccgtaact aagttttttg caacagtaac aatatcatct
     2461 cgcttttcaa cagttttggc ctcgggttct ttaaagataa aaagagttgt aaaaaacatt
     2521 aatgcacagc ttattgcaga aacaagataa acaaactcta ttccaaaaga atctcttaca
     2581 aagtatgcca acaacggacc aatagcggca ccgatgttta cgagccaata ataaattgca
     2641 tatccaagag atttggtttc gtgggttgtg gttagtgcaa ccgttcccag tacacttggt
     2701 ttgataaacg atccgcctat tgccgtaaaa attaatatta ctgaaagaac taagaaaaga
     2761 tttacatcag tgtaccagcc ggagaatgca gcaatacctg ttgatccaat taaaaaatat
     2821 ccgattgcca gaacaaagaa tgcgaatgaa aaagcttttt taaatccaaa tttatcagcc
     2881 aatgcgcctg caaagattgg taaaaaatag atcaatccgc caaagattga ggaaagttgt
     2941 ccggcttcaa cttcggtaaa ttttaaatga tctctaagaa atacgctaag tattgttgcc
     3001 tgcccataat aagctaacct ttcaaaaagc tccattccat ttgccaccca gaatgaatgc
     3061 tgaaaacctg attttagctt ctgcattgtt cccgtttgtt ccacaaattt acctttccaa
     3121 aaataattcg cgcaatttag ttatgctaat ttttagtaac aaagcttttg taagttttaa
     3181 tctttctgat tagttcaatc gttgctgaac aatttacatg ttcataagtt cttcaatgtc
     3241 taatatttac ttcaataaaa acaaaaaact aatcaatggc ataaagaaaa agcttatttt
     3301 agaatagtaa caatttcatt tttcggaatg attcttgtca cgtttatagt aagttaattt
     3361 attgaaattg attattacag aaataatttt taaacatacg caagtaataa aaaaagatta
     3421 aagccggagg cttacataaa gatgaaaaca ttcctaataa cattcctgct gattttatta
     3481 aacacaatta atttttcaca aaacaagaag gctgtaatct ctgagattaa aaatgatgag
     3541 gtaaaagtaa aacttcaccg atttattgaa tttgatgatt caaaagcaaa aagcggcaac
     3601 aaatttttag tggcagatat ttccctcgag aacatttcca gcaaaagcat tgcgatggga
     3661 tctgattata ccatgacaat tacaattaaa gatgataagg ggaacgaata tcgctccggt
     3721 ttgaaaggcg aaggaattgt ttctacatat ctaataaggc aggacagtga agaacaggac
     3781 accaaggcac ataatctgtg ttttagtgat aattttccgg caaaaacaaa agcaagatcc
     3841 ttgttgtgtg gattcgaagt cccaaaagac gcaaaaattg tttcattcgg tttaaagaag
     3901 aaaaacttat ggagtgccat taagtaaatc attttcaaaa gtaaacaaac taacaaaggc
     3961 gattttaaat aatcgccttt ttcatttctt agaaatcaca attctttttc cattatttga
     4021 taaaattcat tttgtattta tcaattattt cattggttta atcgacttaa acacaacatt
     4081 tttatttcat tgtgtggcat acttttggaa aaaatgcaat gctgaatttt ttatttattc
     4141 actattgcta attattaatt tactgataac tgatacatat gaaatcaaga acttttcacg
     4201 gcggcgttca tcctaaagaa tataaagagc tgactgagca tagtgccttt gaatttatgc
     4261 cctatccgga agaaataata cttcctcttt ctcaacatct tggtaaagaa gcatctccgc
     4321 aggttaaaaa aggtgatgaa gtgacccccg gccaattggt tgcaaaggcc gagggattta
     4381 tttctgctcc cgtgcatact tctgttgctg gaaaagtaat gagtcttggc aaagaacaaa
     4441 catcaagcgg atttccaaaa gactcaattg taattaaaac aaacgggact gtcgaatcag
     4501 aaaaatttct ttttcctcct ttaaatcctg acaccattac agctgatgaa ataagagaac
     4561 gtgttgcact ggcaggaatt gttggacagg gcggcgctgc gtttccaact tcagttaaac
     4621 ttactctgcc aaaggataaa gtaattgatg ttgttattct taacggatgt gaatgtgagc
     4681 catatcttac acgcgattac agatttatga tcgaacgacc cgatgatctt atatcgggac
     4741 taaaattaat tatgaaagcg ttaaatgtta atcgcggggt aattggaatt gaagacaaca
     4801 aaccggaagc gattgaactg ctttcaaaca aagttaagtt tgaacctgga ttagaagtta
     4861 ttgcccttaa aacaaaatat ccccagggcg cagaaaaaat gttgattaag gcagtaaccg
     4921 gcagagaagt tccacccgga aaattgccga tggatgttgg agcagttatt cagaatatcg
     4981 gaacagcgct tgcaattcac gatgcaataa taaaaggcga agttttaatt acagctgcgc
     5041 ttactgtttc cggtaaggga attaaaaatc ccaaaaacct gatcgtatct gttggcactc
     5101 caattcaaaa cgttattgat ttttgcggcg gagtaacaga agatgctgtt aaagttgttg
     5161 tgggcggacc aatgatgggt gttgcccagt ttgatcttca cgctccggta atgaaagcaa
     5221 catccggaat tttagtgctt acgaaagatg aagttgctga aaatcctgaa acaccttgct
     5281 taagatgcgg acaatgtgtt ggtgcttgtc ccttaaactt aatgccgaca aaacttgcga
     5341 gatattccca gttaaacaga tttgaggatg ctgaaggtgc gggaataaca gtttgtatgg
     5401 aatgcggaac ctgtgcttat acatgtccgg caaatatccc ccttgttcaa tggataagat
     5461 taggtaaaca aaaagtttta caaatgcaga aggaaagaat ggggaaataa aaatgtcatc
     5521 ccgaacttgt ttcgggatct ttaaaacata aagagatgct gaaacgagtt cagcatgact
     5581 attataaaga aattatggaa acaactacca ctacagaacc aaaacaaaaa gtctattttg
     5641 atttaataac atcacctcac ttgcattcaa aatggtcaac atcaaaagtg atgtggtttg
     5701 ttgttgcagc attagttcca tcaattgcag cggcactaac attctttgga tggacacaga
     5761 tctttataat aattactgct gttgtatttg cagttggaac cgaagcatca attcaggcga
     5821 taagaaaaaa acaaattacc tggtttgatg gaagtgcagc tttaacggga ttacttctgg
     5881 cattaactct tcctccaaat ttttctttat ccgcaactgc tattggttcc gttattgcta
     5941 ttggattggg caaacaaatt tttggcggac tgggttataa tatttttaat cctgctttgg
     6001 ttggaagagc atttttgcag gcggcttttc ctgttcaaat aacaacgtgg acaaaaccaa
     6061 actttgctgt tgattcggtt acaagcgcaa ctccacttgc cgcattaaaa tttgataaaa
     6121 tatttgcaga tacatcatca ttagcaatag gaaatattgg cggatcactt ggtgaaactt
     6181 ctgcaatagc agttttgatt ggcggaattc ttttaatttc agttggaatt gttaactggc
     6241 ggatcccgct ttcaatgata atcggaatgt ttgtgtttgg cggcattttc tggttgataa
     6301 atccacagaa tccaacgcca gtctttcaca tctttgcagg aagttttctg ttcggagcaa
     6361 tgtttatggc aacagattgg gttacatcac caataacaaa caaaggaatg tgggtttttg
     6421 gattagggat ttcacttgtt cttgttgtta tcagaattta cggcggctta cccgaaggag
     6481 ttatgtattc tattttattt atgaacggat ttgttcctct tattaacaga tacaccagac
     6541 caggaatttt tggtgctgta aaggagggaa aaaagaaatg aaagtaacgc atattgttgc
     6601 agcattaact ttaattggaa ttgttgccgg gggctcactt tctcttgtta ataactgggc
     6661 ggcacctaaa attgcattaa accaaaaagc agaaacagaa cgtgcaattt ttcttgtgca
     6721 tcttgatgga aaaagatatg atgaaattaa gaataccgga tttgaagttt ataaagtgtt
     6781 tgatgaaaaa gattcttcag tcggatattc acttgtttat gccggcaatg gatttcaggg
     6841 aaaaataaaa ttaatgattg gcttaactga agatttaaat aaaataactt caattgaagt
     6901 acttgagcaa tctgaaacac ctggactcgg cacaaaaatt cttgaaccgc cttacaaaga
     6961 tcagtataac gggctaatac cggtaccatc cattaaactt gttaaaggag ttgagccatc
     7021 aaatccaaat gaagttaagg cgattacagg tgcaacaatt tcatcgcgtt ccgttgttgc
     7081 aattgttaat gaaggagtaa gtaagttgaa agagcttgaa tcaaaaggag cattgaaatg
     7141 aaaaagcagg taacgctcct tagagaattt acaaaaggtt tgtgggagat taatcccacc
     7201 tttaaacaaa tacttggaat gtgcccaacg cttgcagtta cagtttctgc tattaacgga
     7261 attgcaatgg cgcttgcaac aacatttgtt ttgttcttct ccagcctgat tatttcatta
     7321 ataagaaaat ggattccaga tcaggtaagg attgcttctt acattgttgt tatagcaacc
     7381 tttgtaacaa ttgttgatct tgtaatgaaa gcacaatttc cggatttaag taaagcgctc
     7441 ggtcctttta ttccgcttat tgtcgttaac tgtattattc tcggaagagc agaagctttt
     7501 gcatcaaaga ataatccttt aagatcgatg cttgatgcgc ttggcaacgg agtcggattt
     7561 ttaatttcac tttttgctct tggaagcatt agagaaataa tcggatcaag aacaattctt
     7621 ggctaccagg ttatgccaaa tggatttgaa ccatggttaa tcatgatttt acctgcgggt
     7681 gcatttttaa cgcttggttt gatgatggga tttgcaaatc tatacgttga gagaaaaaag
     7741 aatcttgaaa gagaagctct tattgcacag tacaaacgtg ttggtcgaga agaaattaca
     7801 gatgaagtat tgaaggaagc aggggtgtaa tatggaatta ttaataattt ttatctcggc
     7861 tgccttagta aacaattttg ttctatcata ctttcttggt atctgccctt ttattggggt
     7921 atctaacaaa ttaagctcgg caatatcaat gggaatggcg acaacatttg taatggcttt
     7981 aacagcaatt gtaagctggt tgttgtataa ccttattctg gttccgtatc atctggattt
     8041 tttacaaata ccatctttca ttcttgtaat tgctgcatta gttcagtttg ttgaaatggt
     8101 tataaaaaaa gtaagccagc ctttgtaccg cgcactcgga atttttcttc cattaataac
     8161 gaccaactgc gcaatattag ggttagcatt gcttgcttcg atgaggcaat ataattttat
     8221 ggaaagcgtt attttcggag ttggtgcggg tgcaggtttt acgcttgcat tgattattat
     8281 ggctggaata agagaagaac ttgatcttgc agatgtcccc aaaccattcc aaggtgcgcc
     8341 aattacatta attgttgctg gtattttagc tcttgctttt atgggctttg cgggcatgat
     8401 atgatgttag tgagtcgcag gtggttggta gtaagtagtt tatgtcacac tgagcggagt
     8461 cgaagtgtga aaagtaattg tacattgtaa attctacatt gaggaaaaaa tgatatcacg
     8521 aagaaatttc ttaaagattg caggcctatc atctgttgca ttaggcgcag gatttactgc
     8581 cggtaaactt gttggaaata aaaaatcagt ttactttgca gtgcatggat tcattcctgc
     8641 tgatgaacag gtaattgcaa atcttgttac agcatttaag aataaaacaa aatgcaatag
     8701 taatccggta attgtttcaa aaacaaagat tggcgagacg atcaatcgat ttgatttaaa
     8761 tgccagaaaa gaaaacttta aaaatagtgg ctcagttact tacagaattc aaagattaga
     8821 caaacaaata gactccgatt taattgtaag tgatgccaac aattctgttt attctttaaa
     8881 tgatctgaat tcgtcatttg aaaatatcag atccggttta aagaatagaa aagcagatct
     8941 tctttttaca gcagagtata aagaaactga ttttctatca actttgctga attcagatag
     9001 aaaagaagtt gtgattgaaa atgaaaaagg acttgttgat agaatatcct taaacaatac
     9061 ttataaaaat attcttattg gtggtccaca aggtaaaacg ggactaaaaa ttgaaaacgg
     9121 aactgcacag gttcatactt caacttgcag acatggaatt tgtaaacatt ctgttgcaag
     9181 cggacctgga aacattattg cctgtgcgcc gaataaagtt ttggtaaaga tcgagcgatc
     9241 atagtttgaa aaataaaata gaatatggtg ttcttgttgt tgttcttttt cttattggat
     9301 ttttcattgc aaaaaatagc agcgatgaat taaaaacatt caagcgaaca caaatcctgc
     9361 ttggaacagt tgttgaaatt caggtgcgag atgttgatga aaaaaaagct gatgatgcaa
     9421 tttcaaaagc atttgcagaa gtaaaaagaa ttgatgattt gttcacaact tttaatgatc
     9481 gaagccctgt ctggcaaatc aatagctcgg tggataccat tattgatgtt gaacctgaaa
     9541 tctacaactt aattgttctt tgtgattcaa tcacaaaatt atctgatggc ggttttgata
     9601 taagtcttta taatcttaca aaagcctggg gattttatac agataatcct catcttccat
     9661 ctaaaggggc aattgactct gctttgctag taagcggctg gcaaaagata aatttgttag
     9721 ataaaaaccg aattgaaaag aaagaaaagg ttgaattaaa ctttggcgct atcgcaaaag
     9781 gttacgctgt tgatagagcc atcgaagtcc ttaaaagatc tggaataaca caagcactgg
     9841 taaatgcagg cggagaaata agtgttcttg gtaacgattg gattgtcggg attcagcatc
     9901 caagagaaat caattcgatc attaaaaaaa taaaacttga tgaatacaca gttgcaacct
     9961 caggtgatta tgagcagtat tttgaagttg attgcgttcg ttatcatcac atacttgatc
    10021 caaagacagg ctatccttca agaggcttgc aaagcgttac gataataaat aaatcaaaca
    10081 cttttgccga tggattagca accacgattt ttgtaatggg agaagaaaaa ggaatgcaat
    10141 tggttgaaag tttgaatgat actgaagcta tgataatcga tagcgcagga aagatttttt
    10201 attcatctgg tttcgaaaag ttcttagtaa attgaaataa gtatatcatt gcgagtcccg
    10261 atttatcggg acgaagcaat ctttaataca gattgcttcg tcacattcgt tcctcgcaat
    10321 gacggacaag taagaaggaa taaaatggac ataacattaa taatagcaat agccacaatg
    10381 ggcgggcttg gatttatttt tgcaggtgct ttagcatttg ccgataaaaa attacgcgtt
    10441 gaggaagatc cgaaaattgc gcaagtgaat gatgtattac caaacgcaaa ctgcggtgct
    10501 tgcggaaacg ccggctgcta tgattttgca gttaaagttg ttaatggtga agcaaagata
    10561 agcggttgtc cggttggtgg acaggaagtg gttgatgaga ttgcaaaaat tcttggaatg
    10621 gaaagcactc aaagcgtaaa actggttgca cgaattcttt gtaatggggg aaatatagaa
    10681 gccgtaaaaa aagaaggaat agaatatctt ggtcctcaaa gctgttctgt aaaaacaatt
    10741 gttgcaggcg gagataaaat gtgtttgtac ggatgtctgg gcggcggcga ttgcgttgat
    10801 gcatgccagt tcggggcaat atttatgaat gagaacggat tgccaactgt tgtggaagaa
    10861 ctttgcacgg gctgcggtca atgcgttgac gcttgtcccc gaggagtaat tgaaattcat
    10921 cctgaaggca gagagttgtt tattttctgt aaaaatcatg atgacccaaa gagatctaag
    10981 gaagtttgta atgttgcctg ctttggctgc ggaatttgtg cacgtaaatc tgatggttca
    11041 attgagatga gagattttct accggagata gattacacaa aattggattt gagtaaaatt
    11101 ccaattgata aatgtaaaac cggagccata agattaattc atcctgaaag aacgacagag
    11161 ttattaaaag aagagaatat tgaagtaaaa gtaagttaag attaatattg tcattcccgc
    11221 gaaagcgggg atttaaaatc aaatattgag attccgcatt atgcgcggaa tgatagaaaa
    11281 ataaaatgta caaagaagaa ctttacgaag acggaatagt aaaagaatcc caaaacggat
    11341 ttgcaacaat aattatttca gattctgatc attgtataga atgctcggca aagctttatt
    11401 gtaaaccagg caattcaaat gaaagaagtc tgacggcagc tgattcttat ggagctcatc
    11461 ctggggataa agtaagagta tcaattagcg ggcgtaaaat actttccgct gcatttttgt
    11521 tgtatggaat tcccctgata ttattaatgg caggactttt atttggttca aagatcttca
    11581 gctatcatca agaactattc tccacattat tagctataat gcttatttca atttacttaa
    11641 tcgggttgtt tttcttttcc aaacataaca aacataatgt ttcgtttcat ccgcaaatta
    11701 cttttataag ctcatcaacc gtttaacaat atttaaattt ttaaaatatt tccttgctgc
    11761 ggggcaaaaa aattatatat attcctgtca ccaataacta attctaatta acattataca
    11821 ggaggcacaa tgaaaaaatt ccaagttttt cttttcgtat tatttataac ttctggtgcg
    11881 gccattttta gtattgctta tggcgaacag ccggtttttg taaaagaatt catcgggcag
    11941 atggattatg tacagggcaa aattttacaa ctggaagatg cgatgccgca ggataagttt
    12001 agctggagac cggaagaagg ggttcgttct gttggtgaag tcaatcttca catggctttc
    12061 agtaattatc tttttataaa aactcccgga ggcacaccac cgccagatat aaatatggat
    12121 tttaataagt gggattatca gacaaatgat aaaaaagaaa ttgcggctat aattaaaaga
    12181 tcatttgatg atgcaaaaaa gtatgcttcc ggtttaacag aaaatgacct taataaagag
    12241 gttgattttt ttggcaacaa aatgacagta agaaatttta tggtgactat gcttaatcat
    12301 ttgcacgaac atcttggtca gtcaattgct tacgcaagaa tgaataaaat cacaccaccg
    12361 tggagtaaaa aaggagaata gaaaatatta agaggctatc tcaggttaat tgatttttct
    12421 gaaatagcct cttatttaat taattaagtt tatttatctc tgcttcaagc ttatcaacaa
    12481 gctcactgaa ttgtgaaatg acagctttaa ttgtttcagg tttagttaaa tcaactcctg
    12541 ctttttttag ctgttccatc gggtaatcat ttccacccga tttaagcaat gtaagatatt
    12601 tatccattgc ctctttcttt tcagattcac ttcctttaaa aatgttatta taaatctgtg
    12661 ctgatgatgc aaagcatgta gcatactgat aaacataaaa aggtgttctg aacatatgag
    12721 gaattcttgc ccatacgtga tttatgaatt catccttctc aattgagtcg ccataatatt
    12781 tatcatatag gtcggacatt atatttgtta atgtttctga agtaacagat tgaccttttt
    12841 caaccaaagt atgaaccttt aattcataat cagctaaaag agtttgaaaa taaaaagtac
    12901 ctgtaactcc ctgtatagct tgctgcaaaa gattaattct ttctttcgga tctgttgttt
    12961 gggataacat ataatccagt aaaagtcttt cattaaaagt tgaagcaacc tctgcaacaa
    13021 atattgtgta agatgaagtt gcaaaaggct ggttttcctg tgaatagaga gtgtgcattg
    13081 tatggcccag ttcatgtccc aatgtaaaaa cattatcaag agtttcgttg taattcataa
    13141 gcatataagg atgaacaccg taaacacctg cggaataagc tcctgctcgt ttacctttat
    13201 tctcaaaaac atcaatccag ccgcccttta aagcaccgga aagtttttct ttatattctg
    13261 cgcctaacgg agatacagat gcaaggaccc attcttttgc ttcttcatag ggataggttt
    13321 ttgtgaaatc tgttaaggga attgatccat cgaaactgta gtatttatcg agtccgagaa
    13381 gttttttacg caatctgtta tatttttgaa gaggttcagt atttgcctta actgtattta
    13441 caaggttttc ataaacttca actggaatat tgtttccatc aagctctgct tcaagagaag
    13501 agttatatcc tcttgctctt gcagaagccc aatctgcctg gcagacagcc gaataaattg
    13561 aagcgtgtgt gttttcattt ttcttaaaaa ctccgtaatg agcttcaaat attttttttc
    13621 tgtcttcctg gtttttatct gttgcaagta ttcttccgta atttccataa gttgcctgta
    13681 ctgaatcacc attggaaaga actacggttg gaaaatcaac atccgcagtt gttagatttg
    13741 aatatatatc gctcggtgaa ctgcgcagcc tcgaaaagta ggaaagaagc tcttctttat
    13801 ctgcggaaag aacatgcatc tgctgtctgt aaagcgtttc aattccaaaa cggtaaggct
    13861 caaaagcagg attactattt aaccatttat tcattgtatc ccatggaatt tctagtaatt
    13921 caggatttat ccaggaagtt gcaaccccaa actcagcaaa cattgcctga actttctgaa
    13981 gatttgcacc catctgctga ttgcgtgtat caagatccat atgaagctgt ggatatctgt
    14041 aaattttgta agaaagtatt ccaagctgat cattaagctg cataaccttt aacaggtttt
    14101 caggattttc tttaagcttt cctttgtacg aaataatttc atccattttt gtttttaaat
    14161 ttgtcagatc attttcccaa tcattccagt ccggataaat atcatttaaa ttccatttat
    14221 actcggaagg tatttcatcc ctgttttcat aaactttctg agcatatgct ccttcagcag
    14281 ttaaaaacga tactagcagc agcaaaacat atttaaccat aataattctc catttttatg
    14341 tttgattatt tattaaccaa gttaaagtct tatcagttgt ttatcaaaac aatataaacc
    14401 taaactctat tctttttgat taagggctca aaattaagat taaaggatta agcttcgctc
    14461 aagaaatttg ctattcagtt atagtcctta ttccacttac cataagaatg cttactaaaa
    14521 gtaaaacagc accaatccat tgaacaggtg aaaggatatt cccgtgaaca aagtattcca
    14581 gaattatagc agtaagcgga aatgcgagtt cgcaaataga ggccactgaa gctgatatat
    14641 gttttagtcc gtaatagtaa aggaaaattg cagcaccgcc tgttgtaaaa gcaattatta
    14701 aaaatataat aatttgtttt gtggtgatct ctgtaatact agaaatatct cccgttgttg
    14761 aggctataat aagcattatg attgcagcaa ataaaaatct aagataagtt cccatctcat
    14821 aatcaacatt acgtaatgct cgcttactta atacagttga tgaactaaaa ctaaatgcgg
    14881 caagcaaagc aaatagagcc gcaattgtag ttttatcacc cgtggagaaa tttggaagag
    14941 ataaaccaaa agtcataaag tatgctccaa caattgctaa tcctgcccaa agaaaaaact
    15001 ctttaggaag tttttctttg agaaaaatag tagcaagtaa aattgcaaat accggctgca
    15061 gtttttgaag aagaataaca actgatagat taacaaagtt tacatagaaa agtgcttttg
    15121 taattgccat tgtaccaact gcaccgccga gcagcgcaac accgaagaat gctaaccagt
    15181 cttttttagt taaatgtttt agcgatgaaa atttatttat aaaaaatggg gtaagtagaa
    15241 cagcaacaat cgcgctttca actaacacaa ccagaggaac aggcaaacta taaagcgagg
    15301 gacgcaaaat aattccatct acagcccaga gacttgctgc aattattact aacagtgagg
    15361 gaaataactt tgtgttatac tctattttat ttttttcaat caagttttat ttcaattaga
    15421 tttatcggaa gagaatacat ccagtctaag tttagtaata aaaatataga ctgcaataac
    15481 tatcgtaata gcattggcta taattatcgg ataggataga attaatattc cataccaaag
    15541 ccataaaaca aatcctgcat ttaataaaga aaacattccc agtgatatgt ctttagtgtg
    15601 ttttgattta tatactttta ttgcttgcgg aatgaatgca atagtagtac aaaaagctgc
    15661 taaatagcct aaataggatt ccatatttct ataaataatt tgagccgtaa acttataaat
    15721 aaatgaataa ttatttgtta attctttgct tatagcaact cattcggcta agtatcacct
    15781 gactatctct ggtaaaaatt acccggattt attgaaacat tacttttttt tcatatcatc
    15841 gcttagaaaa tcttacagaa tctcaatagt aaattggaac aataattgca caaatctacc
    15901 caaaatagaa taatagcttt tgatgaaatt tggtgatttg tttaaaatct ttctaattaa
    15961 atcatcaatt gttttttaat aaaccaattt gaggcaatta cgtgcaagac cagctaacaa
    16021 aagaaactat ctctggtaaa tttaccgagg attttgattt agatattgaa aaaacaaata
    16081 ataaaaacat cgttccggct gaattaccta attggaataa agcggaaact tctttacaaa
    16141 ataaacttca gtttgtttta agaagagtaa atctctccaa atcatttttg gatgatgacg
    16201 tttctaaatg ggtgaacgca tccaacgagc cttatgaact ttcagcatta cgcacgtatc
    16261 ttctattaag ttgtgttaat atgattggag aaatatgccg aactgatact ccaatgacat
    16321 ttctatcctg gcttgacaca tcaaactcag tgacaacaag aagtataaga gaagagtcta
    16381 tcagcaaggc aataaattct gttggagtta ttgatgatca gaaaaaggca gttaaactta
    16441 taaaatctgt ttttgcacaa tacaaattaa ctaactcagt agactcaaat tatttttcat
    16501 tctttacaga tgcgcttgat gttgaaacac aaaattggat tgcttcaaac agctggatat
    16561 atcaggataa tcctttagag aagtgggcaa atcttcaaaa tgttaaagaa ggttatgcaa
    16621 acttaacttc tgcagaaaat aaaagattaa caaactctcg caaaagatgg gatggattag
    16681 atataaatca aaaattattt gaagtatcac aattcttaga atttttattt aaccagtata
    16741 atttatgtca ggtaaattct ccggttaaaa tggataaaga tcctgtagca aaagtctgga
    16801 aggatattga tcaggcggtt agtgaatata aagttaatta tttatcaaac tcattgttta
    16861 atcaaatggg agcagatggc cagaagttca aattcaatga ggtcaggtgt gtgtttaaag
    16921 aagaagagtt aatagttttt gaaggagacg ttagagaaaa agatagagag ctctggttaa
    16981 agagactttc agaatgggta aaaacaaaga ttgagtcaga aagtgcgatc ggtgaaacaa
    17041 tctttttagc ccctaaaaat caggctctgg tgctatccgg cgaaaatctt gccgcattac
    17101 ttgaaaagtg gattgacaga gcagttaaga atattttaaa taaaaaataa actttagaat
    17161 aggctaataa gaaaaaaacc atggtgcttc catggttttt ttatttttaa aataaaacaa
    17221 gcatttaact ttccgctaaa cgtattgcca atctttttgt agttgtaaag tgagtgttaa
    17281 aaacagtggt tgctgatttc ggaatatgaa catcaatcgg tggttcttta tataaaaatt
    17341 ctagggttaa ttggtttgga ttttcttgta agttcttttt agattttttc tttttcatat
    17401 ctgcaagtac taagaaaatt tattttattg tgttacaaaa ttcgtggtgt taaactaaaa
    17461 atattaattt ataaaaagaa gaaacatgaa aaattaataa ttttattttc ttcaaaaata
    17521 attttttcaa aaagttgtaa taagttttta caggttttta tttattagtg ttaaaacatt
    17581 aacaacagtt tcaaatcagc atcaaaaaat ttttaatcct aaataaatat ttctattgct
    17641 ttatcagaat gatgcagcaa aaattatttt ctgtcgtaat taacactctt taaataatgt
    17701 gccggatcca tatttctaag cataactgaa ttggtagtaa agtttttttc ttcgcccggt
    17761 tcaagcttat caatttttgc tgtggatggt tgagcatcta tcagatattc tataactatg
    17821 ttattcaatg tttcatcact tatgtttttt attgtacctt gatagtgtgc atagtctgtc
    17881 ggtgataatg caggggcatc caaatctacc gaagctctgg ccttgttata ggagtcttta
    17941 atctcgtata cttctttttc tttaataaaa ttatcgtaag caaaatatcc agctatgact
    18001 aatagaacga aaactaaaaa ctttttcata gtttttcctt ttataaattt tatttggttt
    18061 acattttaca actattgaca attaaaaata accgcttata ataaattatt ttcaatgggc
    18121 aattgtagtt gcgaaatttg ctatgcattt tttaacaaaa agtacaccat tatcaataat
    18181 caattatttg gaggcaaaat gaaaagaaat gcggtttttg tagttttagt ttccgtttgg
    18241 agcacatttt tactgttatc ttttgcagga tgtgttcaag aacagcaaaa gaaagaaatg
    18301 act
//
LOCUS       DASWBY010000314         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_14003, whole genome shotgun sequence.
ACCESSION   DASWBY010000314 DASWBY010000000
VERSION     DASWBY010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14003"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VF870_05935"
     CDS             <1..381
                     /locus_tag="VF870_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HEX9251761.1"
                     /translation="IRRIAAIKDGEDVIGNRTKVKIVKSKVAPPFKQTEFDILYNEGI
                     SKTGDVIDMATELGIIKKGGAWFTYGEDRIQGREQFRQKLIEFPDMYKKIEIEIKTKL
                     GLPGFKTESTTEDKIEEKPKGKKK"
     gene            405..1040
                     /locus_tag="VF870_05940"
     CDS             405..1040
                     /locus_tag="VF870_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX9251762.1"
                     /translation="MKIERVSRKDEKNVSVYFDNGERLILSEDTFYNSGLRKGDEISE
                     DRFSFFIEQNILYHIKQRALSFLARRFHSERELLIKLKSKSYEERLIKIVLSDLKEKS
                     FIDDKVFANHFIEEKLKKKRWGKNKIRAALFSKGISTSIIDESLQGFDAEQDQNATAF
                     KLVKKKFESLIKRETDNKKIKQKLISFLLSRGFEYEVSAEAVNKIIKQDFD"
     gene            complement(1021..1464)
                     /locus_tag="VF870_05945"
     CDS             complement(1021..1464)
                     /locus_tag="VF870_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9251763.1"
                     /translation="MEEINKPELEFNQSCVIPYRISNGNIELLLITSIKKQRWIFPKG
                     FIEFNLSAFESAKKEAYEEAGVIGENETVELGSFDLKKKNQTSHVKIFSMEVTKELKD
                     YPEKNLRKRKWFSVADALGNIENSDIKNFIHKLENKIKPANQNPA"
     gene            1675..2493
                     /locus_tag="VF870_05950"
     CDS             1675..2493
                     /locus_tag="VF870_05950"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX9251764.1"
                     /translation="MNKNQKEEKIVSAYEKAADDGIDPRCIPQKLEKPVPEHDEIIYP
                     NYTSEEQSNWKFLYNRQIEFLPGRACDEYIEGASKLNLTEKKIPYLKDLSHIFYKSTG
                     WKVARVPGLIHEQNFFEMLRRKIFPSTDYIRGKEELDYTPAPDLFHDIFGHMPLLTNK
                     TFASFYQMFGEAALKAEGMNRKYLETFHWFTVEFGLIKKPEGMRIYGAGIVSSLGEVQ
                     NALSDKVDVREFDPDKIVLQDYDVWHLQPILFAIESFEQLEQGFKEWTKKAGLI"
     gene            2641..3111
                     /locus_tag="VF870_05955"
     CDS             2641..3111
                     /locus_tag="VF870_05955"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9251765.1"
                     /translation="MALKIGDKAPDFTLFNQDGEPVTLSSFKGKNVVVLFFPAANTGA
                     CTTELCTFRDELKEYQNLNAQVLGISVDTHFALKMFQEKNNYNFPLLSDFNKKVIKDY
                     DVVLDVFVPGKFDYLNTAKRAAFVVDGNGNIKYFEVLANPGNQPDFEAIKKALA"
     gene            3134..3592
                     /gene="bcp"
                     /locus_tag="VF870_05960"
     CDS             3134..3592
                     /gene="bcp"
                     /locus_tag="VF870_05960"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX9251766.1"
                     /translation="MLEVGKKAPDFSLLNQDEKRISLKDFIGQKVVLYFYPKDDTSGC
                     TKEACSFSDDLPKFSKIDAVIIGVSPDSVKSHKKFSEKYKLKFNLLADEEKKVIEKYD
                     VWKEKSMYGRKYMGVERTTFIIDEKGKIIKIFNKVKVDGHNKEVLEALKD"
     gene            3594..>3796
                     /locus_tag="VF870_05965"
     CDS             3594..>3796
                     /locus_tag="VF870_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX9251767.1"
                     /translation="MIYLTRRETFAAAHRLFREGLSDKENYELFGKCSNPNWHGHNYT
                     LEVVVAGNVDPATGFVIDLKKLK"
BASE COUNT         1390 a          521 c          688 g         1197 t
ORIGIN      
        1 atcagaagaa ttgcagctat taaagatggc gaagatgtta ttggtaacag aactaaggtt
       61 aaaattgtta agagcaaagt tgcccctcca tttaaacaaa ctgaatttga cattttgtat
      121 aatgaaggta taagcaagac cggggatgtg attgatatgg caacagaact tggtataatc
      181 aaaaaaggcg gggcctggtt tacttatggt gaagatcgaa ttcagggcag ggaacaattc
      241 agacagaaac ttattgaatt cccggatatg tataagaaaa ttgaaattga aataaaaaca
      301 aaacttggtt tgccaggatt taaaactgaa tccactacag aagataaaat agaagaaaaa
      361 ccaaagggga aaaagaaata gtaagagaat tcagatcaaa ttaaatgaaa attgaaagag
      421 tttcccgtaa agacgaaaag aatgtatcgg tctattttga taacggggaa cgattaatat
      481 tatctgaaga tactttttat aatagcggtc tcaggaaagg tgatgaaatt tccgaagacc
      541 gcttttcttt tttcatcgaa cagaacattt tatatcatat caaacaaagg gctttatcat
      601 ttcttgccag aagatttcac tctgaaagag aactacttat caaattaaaa tcaaaatctt
      661 atgaagaaag attaattaag attgtcctta gcgatttaaa ggaaaaatct tttattgatg
      721 ataaagtatt tgcaaatcat tttattgaag aaaaacttaa aaagaaacgc tggggaaaaa
      781 ataaaatccg tgctgcgtta tttagcaaag gaatttcaac atccattatt gatgaatctc
      841 ttcagggttt tgatgctgag caggaccaaa atgcaactgc atttaaatta gtcaagaaaa
      901 aatttgaaag tttaataaaa cgtgaaactg ataataaaaa aattaaacaa aaattaattt
      961 cttttttatt aagcagggga tttgagtatg aagtatctgc tgaggccgtg aacaaaatta
     1021 ttaagcagga ttttgattag caggtttgat tttattttca agtttgtgta taaaattttt
     1081 tatatcgcta ttctcaatat ttcccagcgc atctgctaca ctaaaccatt tccttttgcg
     1141 aagatttttt tcaggatagt cttttaattc ttttgttact tccatagaaa atatttttac
     1201 gtgagaagtt tgatttttct ttttcaaatc aaaggaacca agctcaacag tctcattttc
     1261 tccgataaca ccggcttctt cgtaggcttc ttttttagca gattcaaatg cagataaatt
     1321 aaattcgata aatcctttgg ggaaaatcca tctttgtttt tttatagatg taattaataa
     1381 aagctcaata ttaccgtttg aaattctgta cggaattacg caggattgat taaactctaa
     1441 ttcaggttta tttatttctt ccaatattat ctttaatcag tgaacaatta aaatttaatg
     1501 aacttaaaat attttctcgg caaagataaa atttagttga tctatatgaa aatgtttttt
     1561 attggatgag tttaacattt ctaaattgat taaacataaa tctatttatt aattctcatt
     1621 gaaaatttac aaaccaatct ttaacttcaa caacaagaat aacaggaatc tgaaatgaac
     1681 aagaatcaaa aagaagaaaa aattgtatct gcatatgaaa aagccgcaga tgacggtatt
     1741 gacccaagat gcatccctca gaaattagaa aaacctgttc ctgaacacga tgagataata
     1801 tatccgaatt atacttcaga ggaacaatcc aactggaaat tcctttataa tcgtcagata
     1861 gagtttttac ccggcagggc ttgtgatgaa tatattgagg gtgcaagtaa actaaatcta
     1921 acagagaaaa aaattcctta tctaaaagac ttgagtcata ttttttataa atcaaccgga
     1981 tggaaagttg cccgtgttcc cggattgatt catgaacaga atttctttga aatgctgcgc
     2041 aggaaaattt tcccttccac agattatatt cgtggtaaag aggaactgga ttacacgcca
     2101 gcacctgatt tattccatga tatatttggt cacatgcctc ttcttaccaa taaaaccttc
     2161 gcctcttttt atcaaatgtt tggggaagct gctttaaaag ctgaaggaat gaatagaaaa
     2221 tatttagaaa catttcattg gttcacagtc gagtttggtc tgataaaaaa accggaagga
     2281 atgagaattt atggagcagg aattgtttca tctttaggcg aggttcagaa cgcactttcg
     2341 gataaagttg atgttagaga atttgatccg gataaaattg ttttacagga ttacgatgtt
     2401 tggcatttac aacctattct ttttgctata gaatcttttg agcagcttga gcagggtttt
     2461 aaggaatgga ctaaaaaagc gggtctgata taaatcaaat tgaactttat tagaataaga
     2521 ttgttatcta aatcaacatg ttttactatt tcttgaaagt gcaaagcctt ttggctaact
     2581 tgaagtagaa aaacaaaaaa ctttccgata attactaatt aaatcaaata ggagactacc
     2641 atggcactca aaattggaga caaagccccg gattttacat tatttaatca agatggcgaa
     2701 ccagtaacat taagctcatt caaaggaaaa aatgttgttg tattattttt tccggctgca
     2761 aatacgggag cctgtactac agagttgtgt acttttagag atgaacttaa agagtatcaa
     2821 aatttaaatg ctcaagtatt gggaattagt gttgacactc attttgcatt aaaaatgttt
     2881 caggaaaaga ataattataa tttccctctg ttgagcgatt ttaataaaaa agttatcaaa
     2941 gattatgatg tagtattaga tgtttttgta ccgggtaaat ttgattattt aaatacagca
     3001 aagcgtgctg cattcgttgt tgatggtaat ggaaatataa aatattttga agtacttgca
     3061 aatcctggaa atcagccaga cttcgaagca attaaaaagg cacttgccta attttttaaa
     3121 ccggagaatt aaaatgctgg aagttggaaa aaaggcacct gattttagtt tgttaaatca
     3181 ggatgagaaa agaatatctt taaaagattt tattggtcag aaagttgtcc tttattttta
     3241 tccaaaggat gatacaagcg gctgcacaaa ggaagcctgc agttttagcg atgatttacc
     3301 aaaattctct aaaattgatg cggtaattat tggtgtaagt cctgattcag ttaaatccca
     3361 taaaaagttt tctgaaaagt ataaacttaa atttaatctg ctcgctgacg aagaaaaaaa
     3421 ggttatagaa aagtacgatg tatggaaaga aaaaagcatg tacggcagaa aatatatggg
     3481 tgttgaaaga acaactttta tcatcgatga aaaaggaaaa atcataaaaa tatttaataa
     3541 agtaaaggtt gatggacata acaaagaagt gctggaagct ttaaaggatt aatatgattt
     3601 atcttaccag aagagaaaca tttgctgcgg ctcatcgatt attcagagaa ggtttaagtg
     3661 ataaagagaa ttatgagttg tttggtaaat gcagcaatcc aaactggcac ggacataatt
     3721 atactttgga agttgtagtt gccggaaatg ttgatccggc aactggtttt gttattgatc
     3781 tgaaaaaact aaaaga
//
LOCUS       DASWBY010000315         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_612431, whole genome shotgun sequence.
ACCESSION   DASWBY010000315 DASWBY010000000
VERSION     DASWBY010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612431"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VF870_05970"
     CDS             complement(<1..823)
                     /locus_tag="VF870_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9251768.1"
                     /translation="MGQPTVVGEILAGVVLGPSLLGATPYFSDLMISQSSTGTNLLEV
                     VSLIGAMLLLLITGLETDLALIKHHARSAFGTALGGLILPLIFGFLFCFFIPDSFLVD
                     PSQRTVFSLFVATAISVSAIPVIAKVLIDLNLIRRDIGQITIAAGMIDDTAAWILLSI
                     VLGLIEVGVVTAENVFISVGKVLAFLSASIIAGKWIAAKAVSFAQNSIQSRYKFLTIL
                     ILFSFGFGAIAQSLKLEAVLGAFVAGIIFSRIPAVPKEAIERIESFTFGIFAPIFF"
     gene            1287..>2093
                     /locus_tag="VF870_05975"
     CDS             1287..>2093
                     /locus_tag="VF870_05975"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9251769.1"
                     /translation="MTTIVDVFGREILDSRGNPTLEVEVYLEGGVMGRAAVPSGASTG
                     EHEAVELRDGDKSRYNGKGVLKAVENVNEKIADEIIDFDATEQVEIDNLLIELDGTEN
                     KGNLGANAMLGVSLACAKASAEFFGLPLYRYIGGVNARTLPVPMMNILNGGKHADNNV
                     DFQEFMVMPYGAPSFKEALRMGAETFHALKSVLAKKGYNTAVGDEGGFAPNLKSNEEA
                     IEVILEAIEKAGYKVGTDIGIALDPAASEFFIKEKNAYHLFKSAPNQLIPI"
BASE COUNT          770 a          344 c          393 g          586 t
ORIGIN      
        1 caaaaaagat tggagcaaaa attccaaaag taaaactttc aattctttca attgcttctt
       61 taggaactgc agggatgcgg gaaaaaataa ttcctgctac aaaagctccc agaacagctt
      121 ccagttttaa agattgggct attgcaccaa atccaaaact aaaaagaatt agaattgtca
      181 aaaacttata acggctctga atagaattct gagcaaaact gactgctttt gcggctatcc
      241 atttccctgc gataatacta gctgaaagga acgctaaaac ctttccaaca gagataaaaa
      301 cattttcggc ggttaccact ccaacttcta ttaatcccaa aacaattgaa agcaaaatcc
      361 acgctgcagt atcatcaatc attccagctg caatggtaat ctgaccaata tctctgcgga
      421 ttaaatttaa atcgattagt actttagcga taaccgggat agcagaaacg gaaattgcgg
      481 ttgcaacaaa aagtgaaaat actgtgcgtt gtgatgggtc aaccaaaaaa gaatctggga
      541 taaaaaagca aaaaaggaaa ccaaatatta atggtaagat taaaccacct aaagcagttc
      601 caaatgcact tcttgcgtga tgtttaatta aagctagatc agtttccaat cctgtaatta
      661 aaagcaagag cattgcaccg attagactca ctacttctag taaatttgtt ccagtcgatg
      721 attgcgatat cattaagtca gaaaaataag gagttgcgcc taatagtgag ggccctaaaa
      781 ctactccggc aagaatttca ccaacaactg ttggttgacc cattcgctga aacaattctc
      841 caaaaactcg ggcaaatagt agtaataaaa agagttggaa aagaagaata agaatgtcgt
      901 tatgtaatga agcttctaac atcaaaatta taaaacgtac taggatatat tattgaatat
      961 aaattgattt ctatattgta aagtaatgaa aagttagttt agaataaata ttcaatagtt
     1021 agcaagccaa ctgatgatta tcagcccaaa ttgaatggga ttataagtta ttacgataat
     1081 tcgctgtaat tactgcacaa aattttttgt tagaaatact tagctgtttt gtagtgataa
     1141 tttgtttaat aacagagtta ttatttgtcg gaatcgaata ttaaatttgt aaacacaatt
     1201 tcacttctaa agaccattta cctaattttg aaaagcaaat taaataaaaa tttgaattac
     1261 atttagaaaa atcaggagtt attaaaatga caacaatagt tgatgtattt ggaagagaaa
     1321 tattagactc aagaggcaat ccgactttag aagttgaagt ttatcttgaa ggcggagtaa
     1381 tggggagagc agcggttcca agcggtgctt ctaccggtga acacgaagca gttgagttga
     1441 gagacggcga caaatcccgc tacaacggaa aaggagttct taaagcggtt gaaaatgtaa
     1501 atgaaaaaat agctgacgag attattgatt ttgatgcaac agaacaagtt gaaattgata
     1561 atcttcttat cgaattagat ggaacagaaa acaaaggaaa tctaggtgca aatgctatgc
     1621 ttggtgtttc gcttgcttgt gcaaaagcat ccgcagaatt ttttggatta ccactttatc
     1681 gatatatcgg cggagtaaat gcccgcacac ttcctgtccc tatgatgaat attcttaacg
     1741 gaggtaagca cgctgataat aatgttgact tccaggaatt tatggtaatg ccatatggag
     1801 ctccaagttt taaagaagct ctaagaatgg gtgcggaaac ttttcatgct ttaaaatctg
     1861 tacttgcaaa aaaaggttat aacacagcag taggtgatga aggtggattt gcgccaaact
     1921 taaaatcaaa tgaagaagct attgaagtaa ttcttgaggc tattgaaaaa gccggttaca
     1981 aagttggaac agatatcggt atagcgctcg atcctgcagc aagtgaattc tttatcaaag
     2041 aaaaaaatgc ttatcatcta tttaaatcag ctccaaacca attaatcccg atc
//
LOCUS       DASWBY010000316         5359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_48621, whole genome shotgun sequence.
ACCESSION   DASWBY010000316 DASWBY010000000
VERSION     DASWBY010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48621"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VF870_05980"
     CDS             complement(<1..1090)
                     /locus_tag="VF870_05980"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX9251770.1"
                     /translation="MKNFFLTVVTVSIMLLASSNIFSQPTIEVYATGLLNPIGIEIDA
                     AGNLWVGEQGTGSGHTASVSMVTTDHQVHKFMVDLPSSAPAFEPIGVTDVYFDIDGKL
                     LIVTAQNTSGDTLGARVLKVDTTGFVPGQTPLNRRNIESIINLNTLLSNGNPYKITAG
                     FENDLFVVDAYYNKVVRWHRSDGSLSVFSSFAPISQSEAVPTCIVYNDTNFFVGNLVG
                     LPVPTGVARIYNVDLAGNNSVYQGGLTAIVDIAINPIDHSLYALQHAEFGPPWLNNKG
                     RLFRIQNGVVDTLISGMQRPSGMVFNPNGDLFITSIQTDNILKVSNLPTAVEDEVNSV
                     VNNFTLEQNYPNPFNPSTNIRYRISSISK"
     gene            complement(1363..3567)
                     /locus_tag="VF870_05985"
     CDS             complement(1363..3567)
                     /locus_tag="VF870_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9251771.1"
                     /translation="MFDDKLKKLEKENLELKEQLEHYKSVVNELQLLNEIAVATGNSQ
                     NIDQTLNTIVQKILRAVNAEQGSIMLVTDDQKDPFQTFVRKESYSTLKPHYHINISIS
                     GWVLKNEEPLLIKNLAKDSRFNSTREEQKNIKSILCSPIWFEGKIIGIMTMVNKKDKT
                     EFTENDQTLLSIISIQAGQLIKNSQLQQEYFLKTKEAELSRWESEKLKELDKIKTNFF
                     TNLSHEFRTPLTLILGPIEKLLNGKAKGDPSSQYQMMYNHATRLLQLVNQLLDLSSID
                     AGKMKLEIGKYDVVSFMKGIASSFQSFAEEKNITINFSSKNEKLEACFDKDKLQKIFS
                     NLLSNAIKFNKENGLVNITLGEKRNGAYSYLQIEVKDNGPGMDSEVQKNIFDRFYKSQ
                     SLSTVEGSGIGLALVKELIELHYAEIEVRSELGKGTEFRVIIPIDENFYNDKNVFIKK
                     NVRIEDSPGSFVKISETDFVKNETAEEAPFVLIVEDNDDMRRFIKENIQTNFKVIEAI
                     DGEDGLTKAIENIPDLIISDVLMPKLNGYEFCEKLKNDEMISHIPVILLTSKAETNSR
                     IIGLETGADDYLTKPFNSAELLLRIKNLIDQRKKLREKFSREITLEPKEIAVTSIDQK
                     FLTRVLEIVEKNVSNENFSAEDFAENVGMSKTHLNRKLNALTDTSANEFIRTYRLKKA
                     ARLLSGKSGNISEIAYEVGFSNPSYFAESFKKLFGYSPSEYLQKQISPPTIS"
     gene            complement(3580..>5359)
                     /locus_tag="VF870_05990"
     CDS             complement(3580..>5359)
                     /locus_tag="VF870_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX9251772.1"
                     /translation="YAGITPLKEFQIARGDYLVTLEKKGYSSVQRIASSYPIIKEFPV
                     PRRGINISTKMLKANEIDSNMVFVPGGDYKIVSWSLTSLPSVVLNDFYIDKYEVSNAD
                     YKEFISAGGYVKKEYWKYPFIKDGKEISWDEAMKLFVDRSQLPGPRSWVNQDYPDGMN
                     IYPVTNITWYEATAYAEFRGKSLPTVYQWEKAAKNGVPNYQGFTMPWGVINTLAKPDD
                     RANFNGRGTEPVNHYEFGLSAYGCYNMAGNVKEWCMNKSNDGYIQTGGSYEDPYYVYS
                     DFGSYPGFFSSDGLGFRCVINTVIMNNDPAAITINRSPKIPNYLPINNAGYKTLLSFY
                     NYDKKPLNSKIISKSETDFWIKEKISFDCPFGDRITAYLFLPKNAQKPYQCIVWDPHG
                     GVYFLGASADWAAEILYSGNIKSGRALYVIVPMGSSERKWNYGGNMPEYSSVLFRDRV
                     VHWVTEQRVALDYLSTRDDIDLHKLAYNAASTGANGFIVPAVDHRYSTVILTASGIDP
                     GNTKSLPEANPVNFIPHYNVPTYMFNGKYDEVVPYYYCALPLYNLLSQPKEIETVNSG
                     HIPPIEIRVPLTNKWLDKTLGPVKFK"
BASE COUNT         1581 a         1010 c          873 g         1895 t
ORIGIN      
        1 cttttgaaat gctggatatt cgatacctaa tattggttga aggattaaaa ggatttggat
       61 aattctgttc aagagtaaaa ttgttcacaa cagaattaac ttcgtcttct acggcagtag
      121 gcaaattcga aacctttaga atattatcag tttgtataga ggttataaac aaatcaccat
      181 ttggattgaa gaccattccc gatggacgtt gcatcccgct aattagtgta tctacaactc
      241 cattctgaat tctaaataat ctacctttat tatttaacca tggtggtcca aactctgcgt
      301 gctgcaatgc atataatgaa tgatcaatcg gatttatcgc aatatcaaca attgcagtta
      361 aaccgccctg gtaaactgaa ttgttccctg ctaaatccac attatatatt ctagcaacac
      421 ctgttggtac gggtagcccc actaaatttc cgacaaagaa gtttgtatca ttatatacga
      481 tacaagtagg aacagcttct gattgactaa ttggtgcaaa ggaactgaat actgataatg
      541 atccatcact cctgtgccag cgaacaactt tattatagta agcatcaacg acaaataaat
      601 cgttttcaaa tccggctgtt attttatatg gattaccgtt cgaaagaagt gtgttcagat
      661 taattattga ttcaatgttg cgtctgttca atggagtctg accaggaaca aaaccggttg
      721 tatcaacctt taagactctg gcaccaagag tatccccgga agtattttgt gcagtaacaa
      781 ttaacaattt tccatctata tcaaagtaaa catctgttac tccgatgggt tcaaatgcag
      841 gggctgaaga aggtagatcg accataaatt tatgaacttg atgatctgta gtaaccattg
      901 aaacactagc ggtatgtccg cttccggttc cctgttctcc cacccaaaga tttccagcgg
      961 catcaatctc aattcctata ggattcaaga gcccggttgc ataaacttca atggtgggct
     1021 gactgaatat attgctgctc gctaacagca ttatacttac tgtaacaaca gttagaaaaa
     1081 agtttttcat tttattctcc ttatttgatt attaaaaatt aacttccaaa ttcttttaag
     1141 ataatttgac ttattatctg ggaacaaatg tgcagaatag aggatggttt ttctaccaga
     1201 aacagaacaa agattataac aaatgaaaca tcttcaacca ttaatcgaaa ttatcagatc
     1261 tagtagtaca acaagcttac tataaacttg tcaaaatctg actctgtttg tttctttcac
     1321 acttcgactg gctaagagtg acagttaatt ttttataagc aattaagaga ttgttggtgg
     1381 agaaatttgt ttttgaagat actcggaagg ggagtaccca aaaagttttt taaatgattc
     1441 agcgaaataa gaagggttgc tgaaaccaac ttcataagca atttcagaaa tgttgccact
     1501 tttaccggaa agtaatcttg cggctttttt taatctgtaa gttcgtataa attcatttgc
     1561 agatgtgtcc gtgagcgcat ttaattttct gttgagatga gtcttgctca ttccgacatt
     1621 ttcggcaaaa tcttcagcag aaaagttttc attactgaca ttcttctcaa ctatttccaa
     1681 aacacgtgta aggaattttt gatctattga agttactgca atctcttttg gctcaagtgt
     1741 aatttctctg ctgaattttt ctctgagttt tttcctttga tcaattaaat tctttattct
     1801 tagaagcagt tctgcgctgt tgaatggttt tgtcaaataa tcatcggccc ctgtttcaag
     1861 tcctataatt ctgctgtttg tttctgcttt agatgttaaa agaataacgg gaatgtgact
     1921 tatcatttca tcatttttaa gcttttcgca aaactcataa ccattaagtt ttggcattaa
     1981 tacatcgctt ataatcaaat ccggaatatt ttctattgct ttagttaatc catcttctcc
     2041 atctatggct tcaattactt tgaagttagt ttgaatattt tcctttatga atcgtctcat
     2101 atcatcgttg tcttcaacta tcaaaacaaa tggggcttcc tctgcagttt cattctttac
     2161 aaaatctgtt tctgaaattt tcacaaatga tccaggacta tcttcaatac gaacattttt
     2221 ttttataaag acatttttgt cattataaaa attttcatca ataggaataa ttactctaaa
     2281 ttctgttcct ttccctagtt cgcttcttac ttcaatttca gcataatgta attcaattaa
     2341 ttccttaact aatgccaggc caattcctga tccttcaact gtggataaac tttgtgattt
     2401 ataaaagcga tcaaatatat ttttctggac ttcactatcc ataccaggtc cattatcttt
     2461 gacctcaatc tgcaaataag aatatgcccc gttcctcttt tcaccgagtg tgatgtttac
     2521 taatccgttt tccttgttaa actttattgc attggataga aggttgctga atattttctg
     2581 aagtttatct ttgtcaaagc aagcttcaag tttttcattc ttagatgaga agttaatagt
     2641 tatatttttc tcctcagcaa aactctggaa tgaacttgct atacctttca taaaacttac
     2701 aacatcgtac ttcccgattt ccagtttcat tttccctgca tcgatactcg aaagatcaag
     2761 aagctggttt acaagctgaa gtaatcttgt tgcgtgattg tacatcattt gatattgaga
     2821 tgaaggatcg ccctttgctt ttccattcag taatttttcg attggaccaa gtattaatgt
     2881 gagtggagtt ctgaattcgt gtgaaagatt tgtgaaaaaa tttgtcttaa ttttatcgag
     2941 ctctttcagt ttttcagatt cccatctgga taattccgct tctttagttt ttagaaaata
     3001 ttcctgctga agctgggagt ttttgattaa ctgtcccgcc tgaattgaaa tgattgaaag
     3061 cagcgtctga tcattttcgg tgaactctgt tttatctttc ttattcacca ttgtcattat
     3121 gccgatgatc ttcccttcaa accatattgg agagcacaga attgatttta tattcttttg
     3181 ttcttcccgg gttgagttga aacggctatc ctttgcaaga tttttaatta gaagtggttc
     3241 ttcgtttttt aacacccagc cagaaatgct tatgttaatg tgataatgag gttttaaggt
     3301 gctgtatgat tcttttctta caaatgtttg aaatggatcc ttttgatcat cagtcacgag
     3361 cataatagat ccctgttctg cgttaacagc tcgtaatatt ttctgtacaa ttgtatttaa
     3421 tgtttgatct atattttgtg agttaccggt agcaactgca atttcattta atagctgtag
     3481 ttcatttaca acagacttgt aatgttcaag ttgttcttta agttctaaat tttctttctc
     3541 taactttttt aatttatcgt caaacataat tttctaaatt cacttaaact ttacagggcc
     3601 aagagtttta tctagccatt tatttgttaa tggaactctt atctcaatag gtggaatatg
     3661 gcccgaatta actgtctcga tttcttttgg ctgcgataat agattatata atggtaatgc
     3721 acaataataa taaggcacaa cctcatcata ttttccatta aacatataag ttggaacatt
     3781 ataatgagga ataaaattaa ccggatttgc ttccggaagt gatttggtgt ttcctggatc
     3841 tataccggat gctgttaata ttacagtgct ataacgatga tctactgccg gaacaataaa
     3901 gccatttgca ccagtgctgg cggcgttgta ggctaactta tgtaaatcga tatcatcccg
     3961 cgttgaaagg taatccagtg caactctctg ttctgttacc caatgaacta ctctgtcacg
     4021 gaaaagtacg cttgaatatt caggcatatt tccaccataa ttccattttc tttcagatga
     4081 gcccattgga actattacat aaagggcacg accggacttg atatttccgg agtataaaat
     4141 ttctgctgcc caatctgcgg atgcccctag aaaataaact cctccatgag gatcccacac
     4201 aatgcactga tacggtttct gagcattttt aggcaggaat aaataggcag ttattctgtc
     4261 gccgaatggg caatcgaagc taattttttc ttttatccaa aaatctgttt cgcttttaga
     4321 aatgatttta gaatttaaag gttttttatc ataattataa aaagataaaa gtgttttata
     4381 tcctgcgttg tttataggca gatagtttgg aatcttagga cttcgattta ttgtgattgc
     4441 tgcaggatca ttattcatga taaccgtatt aattacacat ctgaatccta gtccgtcaga
     4501 actaaaaaat ccaggataac ttccgaaatc gctgtaaaca taatatgggt cttcataaga
     4561 tccgccggtt tggatataac catcattgct tttattcatg caccattctt ttacattacc
     4621 cgccatatta tagcatccat aagcactaag accaaattca taatgattaa caggttccgt
     4681 tcctcttcca ttaaaattcg cccggtcatc gggtttagct aatgtattta taactcccca
     4741 cggcattgta aaaccctgat aattgggaac tccgttttta gcggctttct cccattgata
     4801 aactgtgggc aaactctttc ctctgaactc cgcataggca gtagcttcgt accaggtgat
     4861 attagttacg ggataaatat tcattccatc aggataatcc tggtttaccc agcttcttgg
     4921 tccgggtagt tgtgatcgat ccacaaataa tttcatagcc tcatcccagg aaatttcttt
     4981 tccatcttta ataaatggat acttccaata ttcttttttt acataaccgc cagctgaaat
     5041 aaattctttg taatcagcat tactcacctc atatttgtcg atataaaaat catttaaaac
     5101 aacagatggc aggcttgtaa gactccagct tacgatttta taatcaccac ctggaacaaa
     5161 aaccatattt gaatctatct cattcgcttt caacattttc gttgaaatat ttattcctct
     5221 tcttggaacg ggaaactctt taattatagg ataacttgat gctattcttt gaacagatga
     5281 ataacccttt ttttctaaag taaccagata atctcccctt gcgatctgaa attcttttaa
     5341 aggcgtaata cctgcataa
//
LOCUS       DASWBY010000317         3866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_424992, whole genome shotgun sequence.
ACCESSION   DASWBY010000317 DASWBY010000000
VERSION     DASWBY010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424992"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /gene="lpdA"
                     /locus_tag="VF870_05995"
     CDS             <1..1308
                     /gene="lpdA"
                     /locus_tag="VF870_05995"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9251773.1"
                     /translation="IDKDNLGGICLNWGCIPTKSLLKNAEIFDIIKNHGKDFGIYTKD
                     LSFNFIEIIQRSRGISDKITKNVELLIKKNKIDRIRGFGKLISQNEIEILDKDGKKTE
                     TVSADNIIIATGARPKTFPNIPVDKKNIITSTEAMSLSEQPKELIVIGAGAIGIEFAY
                     FYSVLGTKVTVIEMLDNILPVEDAEVSQTLEKNFKKRGIEIYTKTMVEKAEVKGNRVL
                     VTINQNGQKKELNADKVLSAIGVTGNVEGFGLEKLGVALFKNHVKVDKKTYRTNIPNI
                     YAIGDVIGPPWLAHVASAEGIHCVEGIKGIKNPDVDYENIPGCTYCQPQVASVGLTEA
                     KAKELGYEIKIGKFPFMASGKAFAVGEREGFVKLIFDAKYGELLGGHIIGSEATELIA
                     EVALARAMEATGHSIIKTVHAHPTLSESIMEAAANAYGEAIHI"
     gene            1418..2113
                     /gene="lipB"
                     /locus_tag="VF870_06000"
     CDS             1418..2113
                     /gene="lipB"
                     /locus_tag="VF870_06000"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX9251774.1"
                     /translation="MIKDAKKLVYCDLGLIDYKDAWDIQKSVHQLRIENKIDDVLFLL
                     EHPHTYTLGKTADKENLVGDKKYLDENNISVYDIDRGGDITYHGPGQIVGYPIISLTN
                     WKQDTHKYLRAIEEVILRVCTEYNLTGSRVEKYTGVWIENRKICAIGIKVSRWITMHG
                     FAFNVNTDLKLFKGIIPCGISDKEVTSLNRELNKEIRLNDIKEKIIHHFDDIFEYDQI
                     EFKSKEEIKSLIS"
     gene            2129..>3866
                     /locus_tag="VF870_06005"
     CDS             2129..>3866
                     /locus_tag="VF870_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9251775.1"
                     /translation="MAKKISTNTKSKEKTSMKQTTGKNDSISVGKHEFTKEKLLYVLK
                     LMLSARNVDNKAMNLLRQGKTFFHIAGAGHEAVQLAIGLSLDPKKDWLFPYYRDLATV
                     LTAGVTPEEVFLGTFGKAADPASGGRQLPVHWGSSRINIPSQSSPTGTQFLQAVGTAL
                     ASVKRGDKNISYVSSGEGTTSQGEFHEAVNWASREKLPVLFVIQNNKYAISVHVSTQT
                     GGKDNSIAEMMEGFQNLHRARIDGTDFFQSYEKIQEAIEYIKSGKGPALIEADVVRLE
                     SHSSSDDQKKYRDKKELEEDLKKCPIEKFAQVLIAKGYLNQNEYDQIKKNINDEISEA
                     ADKALHSVDPSTGDAVKYIFDESGFKESLDYEGSTPSGNKIVMVDAINHALHEEMEKN
                     HEMLIFGEDIEDAKGGVFTATKGLSTRFTKKRVFNSPLAEASIMGVAVGMSLTGMKPV
                     VEIQFGDYIWPAFQQMKNEMATMRYRSNNTWSSPVVTRVAVGGYIHGGLCHSQNIEAF
                     FAHIPGIFIAYPSNAADAKGLLKTACRIKDPVLFCEHKGLYRQSSAISPEPDENYLLP
                     FGKANIIKEGNDV"
BASE COUNT         1398 a          566 c          779 g         1123 t
ORIGIN      
        1 attgataaag ataatttagg tggaatttgt ttgaattggg gctgtattcc aacaaaatct
       61 ttgctaaaga atgctgaaat atttgacatt attaaaaatc atggtaagga ttttggcata
      121 tacacaaaag atctttcgtt caatttcata gagattattc agagaagcag gggaatttca
      181 gacaaaataa ccaaaaacgt agaacttctg attaaaaaaa acaaaattga taggattcgt
      241 ggttttggta aactaatcag tcagaatgaa attgagattt tagataaaga cggtaaaaaa
      301 actgaaactg taagcgctga caatattata attgcaaccg gtgcacgtcc aaaaactttc
      361 ccaaatattc ccgttgataa aaagaacatc ataaccagta ctgaagcaat gtcgctttcc
      421 gaacaaccaa aagaattgat tgttattggt gcaggtgcaa tcggaattga gtttgcatat
      481 ttctattctg tgctaggtac caaagtaaca gttatagaaa tgcttgataa tattcttcct
      541 gttgaagatg ctgaagtttc ccagactctt gagaaaaatt ttaagaagcg cggaattgaa
      601 atctacacaa agaccatggt tgaaaaagct gaagtaaaag gcaatagagt tttagtaacg
      661 atcaaccaga acgggcagaa aaaagaatta aatgcagata aagttttaag tgcgattggt
      721 gttaccggaa atgttgaagg ttttggactt gaaaaattag gagttgcgtt atttaaaaac
      781 catgtcaaag ttgataagaa aacctacaga acaaatatcc caaatattta tgctattggc
      841 gatgtgattg gtcctccatg gctcgcgcac gttgcttctg ctgaaggtat tcattgtgtt
      901 gaaggtataa aaggaattaa aaatcctgac gttgattatg aaaacattcc cggctgtact
      961 tattgccagc cgcaggttgc aagtgttgga ttaactgaag cgaaagcaaa agaacttggc
     1021 tacgaaataa aaattggtaa gtttcctttt atggctagcg gtaaagcgtt cgcagtaggt
     1081 gaacgtgaag gatttgttaa gttgattttt gatgcaaagt atggtgaact tttaggcgga
     1141 catataatcg gaagtgaagc aactgaactg attgctgaag tagcacttgc acgagcaatg
     1201 gaagcaacgg gacattccat aataaaaact gtgcatgcac atccaacttt atctgaatca
     1261 attatggagg ctgctgctaa tgcttatggt gaagcgatac atatataacc tcagcgtaaa
     1321 atcgtcattg cgagtaattc ttcgggcgaa gcaatctaat tttatttaac agattgcttc
     1381 atccctctgg gattcgcaat gacatactaa gaatataatg attaaagatg caaaaaaatt
     1441 agtttattgt gatttgggat tgattgatta taaggatgcc tgggatattc aaaaatcagt
     1501 tcatcagctt agaatagaaa ataaaattga tgatgtactt tttctgttag aacatcctca
     1561 tacttataca ctaggtaaaa ctgctgataa agaaaatctt gttggggata agaaatatct
     1621 ggacgaaaat aatatttcag tttatgatat tgatcgtgga ggcgacataa cttatcatgg
     1681 tccgggacaa atagttggat atccaattat aagcttaaca aattggaaac aggatacaca
     1741 caaatattta agagcgattg aagaagtcat tcttagagtt tgtactgaat acaatttgac
     1801 aggaagcaga gttgaaaaat ataccggtgt ttggattgaa aacagaaaaa tttgtgcaat
     1861 aggaataaag gttagtcgct ggataacaat gcacggtttt gcgtttaatg ttaatactga
     1921 tcttaaatta tttaaaggaa ttattccttg tggaatttct gataaagagg ttacttcact
     1981 aaatcgtgag ttaaataaag aaatccgctt aaacgatata aaggaaaaaa tcattcatca
     2041 ttttgatgat atttttgaat atgatcagat tgagttcaaa tcaaaggaag aaataaaaag
     2101 tttaatttct taaacccaaa aggtttttat ggcaaaaaaa atatcaacaa acacaaaatc
     2161 gaaggaaaaa acttcgatga aacaaacaac tggaaaaaat gattctattt cagttggtaa
     2221 acatgaattt acaaaagaaa aattattata tgttttgaag ttgatgttaa gtgcccgtaa
     2281 tgttgataac aaagcaatga acttattgcg acaaggtaaa acattttttc atattgccgg
     2341 tgcaggacac gaagctgttc aacttgcaat cgggctatca ttagatccga aaaaagattg
     2401 gctatttcca tactaccgcg atttagcaac agttctaact gctggtgtaa cccctgaaga
     2461 agtatttctt ggaacatttg gcaaagctgc cgatcctgca agcggtggaa gacaattgcc
     2521 tgttcattgg ggatcttcaa gaattaatat tccctcacaa tcttcaccaa caggaacaca
     2581 atttttgcag gcagttggta cagcattggc ttcggttaaa agaggcgata aaaatatctc
     2641 ttatgttagc agcggtgagg gaactacatc gcaaggtgaa tttcacgagg cagtgaattg
     2701 ggcaagcaga gaaaaattac cagtactatt tgttattcaa aataataaat acgctatctc
     2761 agttcacgtt tctactcaaa ccggcggtaa agacaattcc atagcagaaa tgatggaagg
     2821 atttcaaaat ctgcatcgtg caagaattga tggaacagat ttttttcaat cttatgaaaa
     2881 aattcaggaa gcaatcgagt atataaaatc aggaaaaggt cctgctttaa ttgaagctga
     2941 tgttgtaaga ttagaatcgc attcatcgtc agacgatcaa aaaaaatatc gtgataaaaa
     3001 agaactcgaa gaagatttaa aaaagtgtcc gattgaaaaa tttgcacaag tgcttattgc
     3061 aaaaggatat ttaaaccaaa atgaatacga tcaaattaaa aagaatatta atgatgaaat
     3121 aagcgaagca gcagataagg ctcttcattc tgttgaccca agtaccggtg atgcagttaa
     3181 atatattttt gatgaaagcg ggtttaaaga atctttagat tatgaaggat cgactccaag
     3241 tggaaataaa atagttatgg ttgatgcaat aaatcatgca cttcatgagg aaatggaaaa
     3301 aaaccatgaa atgttaatct ttggtgaaga tattgaagat gcaaaaggtg gcgtatttac
     3361 agctacaaaa ggattatcaa caagattcac aaagaaaaga gtttttaatt cacctcttgc
     3421 agaagcaagt ataatgggtg ttgcggttgg aatgtcgctt actggaatga aacccgttgt
     3481 agaaattcaa tttggtgatt acatctggcc ggcttttcag cagatgaaaa atgaaatggc
     3541 aactatgcgt tatcgatcaa ataacacctg gtcttctcct gttgttactc gtgttgcagt
     3601 tggtggctac attcacggtg gactttgtca tagtcaaaat atcgaagcgt tctttgcgca
     3661 cattccagga atatttatag cttatcctag taatgctgcg gatgcaaaag gattattaaa
     3721 aacagcatgc agaattaaag atccggtttt attttgtgaa cataaaggat tatacagaca
     3781 aagctctgca attagtcctg aacccgatga aaattattta ttaccattcg gtaaagctaa
     3841 tattataaaa gagggtaatg atgtta
//
LOCUS       DASWBY010000318         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_499679, whole genome shotgun sequence.
ACCESSION   DASWBY010000318 DASWBY010000000
VERSION     DASWBY010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499679"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1113
                     /gene="era"
                     /locus_tag="VF870_06010"
     CDS             199..1113
                     /gene="era"
                     /locus_tag="VF870_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX9251776.1"
                     /translation="MNTKAGYAAIIGLPNVGKSTLMNALLGQKISITTSKPQTTRKRI
                     LGILSEENYQIIFLDTPGILKPAYLLQEKMMDDIDLSVKDADVFVIIYDVSSEQSFKG
                     AEENEYIQKLINEKKNPKILVLNKIDTISQEQALALVERFEKLNIYKSVIPVSANLKF
                     NLERLLNDIVESLPEGAKFYPDDIVSEESERFFVTEIIREKLFELYEDEVPYSCEVLI
                     AEFVERENSKDFISAEIVVERESQKPIIIGKAGRAIKELGEKSRRSIEEFLDREVYLE
                     LRVKVREKWRSNLNMLKSFGYVRAKDKE"
     gene            1225..>1551
                     /locus_tag="VF870_06015"
     CDS             1225..>1551
                     /locus_tag="VF870_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9251777.1"
                     /translation="MKKYTGEVALLFNTLIWGGTFALIKNAFNDISPLLFLGLRFSIA
                     SLIFLPFVYSSLKKTNKQTLIAGSVLGLFYFTGFAAQSLGLNLTSATKSGFITGTFVV
                     FIPILQL"
BASE COUNT          524 a          233 c          279 g          515 t
ORIGIN      
        1 aattttagta attaaaaaat tattgttgtg ctgagctagg ataaggtttg ttttctggca
       61 aaaatgtgct aaccaacttt taaaggatat atcatctttc gaattcgccc actatcacat
      121 tgagtttctt atctacaatc taccttaaat tccttgttca ttcgtatatt tacatatcat
      181 taaaaagaaa agataagaat gaatacaaaa gccggttacg ctgctattat tggattgcca
      241 aacgttggta aatcaacttt aatgaatgct ttgcttggac aaaaaatctc aatcacaaca
      301 tcaaaacccc aaactacccg taaaagaatt ctgggcatat tatcagaaga aaattaccag
      361 ataatttttc ttgatactcc gggaatccta aaaccggcat accttcttca ggaaaaaatg
      421 atggatgata ttgacctttc tgtaaaagat gcggatgtgt ttgttattat ttacgatgta
      481 agttccgaac aatctttcaa aggagcagaa gaaaacgagt acattcagaa attgataaac
      541 gaaaaaaaga atcccaaaat tcttgtgctg aataagatag atacaatttc gcaagagcag
      601 gcgcttgcat tggttgagag atttgaaaag ttaaatattt ataagtccgt tattccggtt
      661 tcagcaaatc ttaaattcaa tcttgaaaga ttattaaacg atatagtaga gtctttacct
      721 gaaggtgcaa agttctatcc ggatgatatt gtatctgaag aatcagaaag attttttgtt
      781 actgagatta ttcgtgaaaa attatttgag ctttatgaag atgaagttcc ttatagctgt
      841 gaagttttga ttgccgaatt tgttgaaaga gaaaattcaa aagactttat cagtgcagaa
      901 atagttgtgg aaagagaaag tcagaaacca atcattattg gaaaagcagg aagagcaatt
      961 aaagaactcg gtgaaaaatc ccgcagaagt attgaagagt ttttagaccg cgaagtttat
     1021 cttgaactga gagttaaagt aagagagaag tggcgttcaa atcttaatat gctaaaatca
     1081 ttcggttatg ttcgggctaa ggataaagaa tagcaaggct gagagttaat aacattatta
     1141 tctttccctg attatcattt tgtcacactg agcgtagtcg aagtgtgact ttactttatt
     1201 tagattttta aattttatta ttaaatgaaa aaatacactg gcgaagttgc gcttctattt
     1261 aatactttga tttggggtgg aacctttgca ctgattaaaa atgcttttaa tgacatctct
     1321 ccgttattat ttcttggatt acgatttagt atagcttcac tgatattttt accttttgtt
     1381 tattcatctc ttaagaaaac aaacaagcag actttaattg ccggatcagt tcttggattg
     1441 ttttacttta caggatttgc tgctcagtcc ctggggttga atcttacttc ggcaacaaaa
     1501 tcaggattta taaccggaac atttgttgta ttcatcccaa ttcttcagct t
//
LOCUS       DASWBY010000319         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_87444, whole genome shotgun sequence.
ACCESSION   DASWBY010000319 DASWBY010000000
VERSION     DASWBY010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87444"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1688)
                     /locus_tag="VF870_06020"
     CDS             complement(87..1688)
                     /locus_tag="VF870_06020"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX9251778.1"
                     /translation="MIRKVKLMEELEAIGIRNTREVFYNYGTPALYEQVIRRREGLLA
                     HLGPLVVRTGYHTGRSPNDKFIVREPESEKNIWWGKVNKGMSAESAERLYFKMMAYIQ
                     GKDLYVEDCYASADEKHKIGIRVVTENAWHTLFARNMFRRYKNDEELSKHKTDFTIIQ
                     MPNFHADREVDCTNSEVFILLNFAKRLVLIGGTSYAGEIKKSVFTIMNYLMPLRGVMS
                     MHCSANVGKDNDVALFFGLSGTGKTTLSADPNRKLIGDDEHGWSDDGVFNYEGGCYAK
                     VIKLSEEAEPEIYECTRKFGTILENVQIDAHTRRLDLDDETFTENTRAAYPLSHLSNI
                     VEDGKAGHPKNIIMLTADAFGVLPPISKLTTEQAMYHFISGYTAKVAGTEKGVTEPKA
                     TFSTCFGAPFMALHPSVYAKLLGEKIVKHKVNCWLVNTGWTGGPYGVGNRMKIKYTRA
                     MLTAALEGKLDNVEYVKDPFFNLMVPQTCPDVPNQVLNPQNTWADKNAYDTQAKKLAN
                     MFVEKFKEYSEGTSDVIKNSGPNKY"
BASE COUNT          515 a          359 c          270 g          616 t
ORIGIN      
        1 atgagaatca ataaatgagc agagctattt ttaatgagcg ggataaacaa aaggcttatc
       61 ccgccattaa aataaaaatt ttagaattag tatttatttg gtcctgagtt tttgattaca
      121 tccgaagttc cctcagaata ctccttgaat ttttctacaa acatatttgc aagtttcttc
      181 gcttgagtat cataagcatt tttatctgcc caggtgtttt ggggatttaa tacttggttg
      241 gggacatctg gacaagtctg cggaaccatt aaattgaaaa atggatcttt aacatattca
      301 acattatcta atttcccttc taaagctgca gttaacatcg cacgcgtata tttaattttc
      361 atacgattac caactccata aggtccgcca gtccatccgg tattaacaag ccagcaatta
      421 actttatgtt ttacaatttt ttcaccaagc agttttgcat acacacttgg atgaagtgcc
      481 ataaaaggtg ctccaaagca tgtactgaaa gttgcttttg gttctgtaac gcctttttcg
      541 gttcctgcta cttttgctgt ataaccggaa atgaaatgat acattgcctg ttcagttgtt
      601 agttttgaga ttggaggaag aactccgaat gcatcagcgg ttagcataat aatattttta
      661 ggatgtcctg cttttccatc ttcaacaatg tttgaaaggt gtgaaagcgg ataagcagca
      721 cgagtatttt cagtaaatgt ttcatcatcc agatctaatc ttctcgtgtg agcatcaatc
      781 tgaacatttt ctaaaatggt tccgaacttt cttgtacact catatatctc tggttcagct
      841 tcctcagaaa gtttaataac ttttgcataa cacccgcctt catagttgaa gactccatcg
      901 tcactccagc catgttcatc atctccaatt aattttcggt ttggatcagc agacaaagtt
      961 gttttaccgg taccgcttaa accaaagaac aaagcaacat cattatcttt tccgacattt
     1021 gcagaacagt gcattgacat cacaccacgc agcggcatta aataattcat aattgtgaat
     1081 actgattttt taatttcacc ggcataactt gttccgccaa ttagcactaa tctttttgca
     1141 aagtttaaaa gaataaatac ttccgaattt gtgcaatcaa cttccctatc tgcatggaaa
     1201 ttcggcattt gaatgatagt aaagtcagtt ttatgtttag ataattcttc atcattctta
     1261 tatcttctaa acatattacg cgcaaacaag gtatgccatg cattttctgt aacaacacga
     1321 atccctattt tatgcttttc atctgcactt gcataacaat cttctacata aagatcttta
     1381 ccttgaatgt aagccatcat tttaaagtaa agtctttctg cactttccgc agacatcccc
     1441 ttgttaactt taccccacca gatatttttt tcactttcag gttctcttac aatgaattta
     1501 tcattaggac ttcttcctgt atggtatccg gttcttacaa ccagagggcc aagatgcgcc
     1561 aataatcctt cacgtcttct gataacttgc tcataaagag cgggtgttcc ataattataa
     1621 aatacttcac gtgtatttct tatgcctatc gcctccagtt cttccatcaa ttttactttt
     1681 ctaatcataa tgttgccttt tagttattta gatttttagt aaaaaaatat tttgtgaatt
     1741 ctagtttcaa actactcagt
//
LOCUS       DASWBY010000320         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_425223, whole genome shotgun sequence.
ACCESSION   DASWBY010000320 DASWBY010000000
VERSION     DASWBY010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425223"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /gene="fabZ"
                     /locus_tag="VF870_06025"
     CDS             <1..534
                     /gene="fabZ"
                     /locus_tag="VF870_06025"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX9251779.1"
                     /translation="VEFAKQIRKLYQQKKLVKKFQFVKKEGVVFDINAIERILPHRYP
                     FLLVDKVIHLEVEKKVIGVKSVTFNEPFFAGHFPGQPVMPGVLIIEAMAQTGGILLLN
                     SLPNFAEKLVYFMKIDKAKFRKTVVPGDQLIMEVELVQKKSSTFVMAGKAYVENVLVA
                     EAEFMAGIVDRPKKAEE"
     gene            537..1313
                     /gene="lpxA"
                     /locus_tag="VF870_06030"
     CDS             537..1313
                     /gene="lpxA"
                     /locus_tag="VF870_06030"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX9251780.1"
                     /translation="MNTIHPTAIVSSKAKIGDNNVIAPFAVIEDDVEIGNDCQIGPHA
                     VLYNGARIANRVKIYQCASIAHKPQDLKFGNEATYFYVGDDTVIHEFVTLHRGTKETG
                     LSRIGKNCLMMAYSHVAHDCVIGDNVIFANGVQIGGHVHIDDWVIIGGTSAVHQFSFV
                     GKHAMIGGNCTAVKDVPPFIICGRNPLKYEGLNKVGLRRRGFSNENIEIIKRTYDLLY
                     NSGLNVSQAIIKIEAELGGNEYAQDVISFIQKSKRGIIGR"
     gene            1310..>1990
                     /locus_tag="VF870_06035"
     CDS             1310..>1990
                     /locus_tag="VF870_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251781.1"
                     /translation="MKWKFLDSGVNSGKFNMDFDVFLADNFRQGNAVLRLYRWNPFCI
                     SLGANQSFEDINLSKTRYHHIDVVKRPTGGRAILHSQELTYSVIYPIEPTSSAKNIYN
                     EINLALRKGLINFDSRLNRIDLEHSQPDFKEFYKSEISAICFAVSAKSELNYKGKKLV
                     GSAQRKLGNVILQHGSILCGAYHKKIVDYLNVADEKKNEMLNEISSTTIDLKSALNIE
                     VDYAKLRTS"
BASE COUNT          689 a          303 c          408 g          590 t
ORIGIN      
        1 gttgagtttg caaaacaaat tagaaaactt tatcagcaga aaaagcttgt gaagaaattt
       61 cagttcgtaa aaaaagaagg cgttgtattt gatattaacg caatcgaaag aatacttccg
      121 caccgttatc catttctgct agtagataag gtaattcatc tggaagtaga aaagaaagtt
      181 attggtgtta aaagcgtaac atttaacgaa ccgtttttcg caggacattt tcctggccaa
      241 ccggtaatgc cgggcgttct gataattgaa gcaatggctc aaactggcgg gatacttctt
      301 cttaattcac ttccaaattt tgcagagaaa cttgtttact ttatgaaaat tgacaaagca
      361 aagtttagaa aaaccgttgt accgggtgat caactaatta tggaagttga gttagtgcaa
      421 aagaaatcaa gcacatttgt aatggcaggt aaagcctatg tagaaaatgt cctggttgca
      481 gaggcagaat ttatggccgg tattgttgat agaccaaaga aagctgaaga ataaatatga
      541 acacaataca tcctaccgcg attgtaagca gtaaagctaa aattggtgat aataatgtta
      601 tcgctccttt tgcagtaatt gaagatgatg ttgaaattgg aaatgattgc cagattggtc
      661 cccacgccgt gttatacaat ggagccagaa ttgccaacag agttaaaata tatcaatgtg
      721 catctatagc tcataaaccc caggatttaa aatttggcaa cgaggcaaca tatttctatg
      781 taggtgatga tacagtcatt cacgagtttg ttactttgca caggggaaca aaagaaacag
      841 gtctttcacg tatcggaaaa aattgtctga tgatggcata ttctcatgtt gcacacgatt
      901 gcgttattgg agataacgta atttttgcta atggtgttca gataggtggg catgttcata
      961 ttgatgattg ggttattatt ggaggcacaa gtgctgttca ccagttttcg ttcgttggta
     1021 agcatgcaat gataggaggt aattgtactg cagtaaagga tgttccgcct tttattattt
     1081 gcggcagaaa tcctttaaag tatgaaggat tgaataaagt tggtttaaga agacgtggtt
     1141 tcagcaacga aaatattgag attattaaaa ggacatacga tctgctttat aactcagggc
     1201 ttaatgtatc acaggcaata attaagatcg aagcggaact tggaggcaac gaatacgctc
     1261 aagatgtaat ttcatttata caaaaaagca agcgtggaat tatcggtaga tgaaatggaa
     1321 atttctggac tccggagtta atagtggaaa atttaatatg gattttgatg tattccttgc
     1381 agacaatttc aggcagggta atgcagtctt aaggctatat cgttggaatc cattttgtat
     1441 ttctctgggg gctaatcaat catttgaaga tattaatctt tcaaaaacaa gataccatca
     1501 tattgatgtt gtaaaaagac caaccggcgg aagagctatt cttcattcac aagaactaac
     1561 atattcagta atttatccga ttgagccgac atcatccgca aaaaatattt ataatgaaat
     1621 aaacctcgcg ttgcgaaaag gacttatcaa ttttgattca agattaaata gaattgattt
     1681 ggaacattca caaccggact ttaaagagtt ttataaatct gaaattagcg caatctgttt
     1741 tgctgtttct gcaaaaagtg agttgaacta taaaggcaaa aaattagtcg gaagcgctca
     1801 aagaaaatta ggcaatgtaa ttcttcagca tggctcaata ctttgcggtg catatcataa
     1861 aaaaatagtc gactatctta atgttgctga tgagaaaaaa aatgaaatgc taaatgaaat
     1921 atcatccaca actatagacc taaaatctgc actgaatatt gaagttgact atgctaaatt
     1981 aagaacatca
//
LOCUS       DASWBY010000321         3893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_612794, whole genome shotgun sequence.
ACCESSION   DASWBY010000321 DASWBY010000000
VERSION     DASWBY010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612794"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VF870_06040"
     CDS             complement(<1..1010)
                     /locus_tag="VF870_06040"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX9251782.1"
                     /translation="MISAIVLVLFSNQYFAQVPEPDGWYKLKSPVNETLRRLYFVNEK
                     TGWAVSLGGKIINTKDSGNSWEIQNSTVTTPIVDIFFINPNRGWALTYPSEPPFGTSI
                     LKTTDGGNNWIKDSVFFQNEIFSTIFFVDENVGFIGGNGIKKTTDGGLTWLNSFIEPG
                     GVSTLPINKFSFYSKTFGYACGGRVDIAGVVWKTTDGGESWSSIGLSPDQIYDVYIID
                     SLNAISLSGDPELLYPLGIIRTTDAGVNWSFTNLPFFGISFAIDFLDDKNGWSAAGYK
                     FLNSTDGGDTWTEQFIFDSTVVYDLQFVDQYTGFACGENGVLLKYNSNKKNAPDKPIF
                     EIG"
     gene            complement(1156..1953)
                     /locus_tag="VF870_06045"
     CDS             complement(1156..1953)
                     /locus_tag="VF870_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX9251783.1"
                     /translation="MILTLSEIYIYPIKSLGGISVDSAIAEERGLKYDRRFLLVDENN
                     MFMTQRDFPLMALLKLSFSEHGFKVLNTKDGSHIIVPFESDSKEVIKVTIWDDICNAV
                     RVNKDLDNWFSSAINKKCSLVYMPDDEKRLAEKKYINEEHIVSFADAYPYLVIGQSSL
                     DDLNSRFAIPVPMNRFRTNFVFSGGNPYEEDGWKDFRIGAAEFKAAKPCARCVITTTN
                     QDTAERSNEPLKTLSEYRNINNKVMFGMNVVCNKTGKIFVGEKIILF"
     gene            complement(1955..3181)
                     /locus_tag="VF870_06050"
     CDS             complement(1955..3181)
                     /locus_tag="VF870_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9251784.1"
                     /translation="MWIILRVLIGVVVLALLEYYFTRKVNRVFKTLFPDYPRKKKYII
                     IYSFLVVLNLYPVILIFNAVYAAITKESVAFPENIFADYLLLYPFWFLIILILQVSVL
                     FVFIDLLKLIFYQLYKKHKEKLYRLQVKIFLALIVFFSVYIPARIIYDYNSVDVRSVD
                     YKKQNLPKELAGFKIAFISDLQADRYTDEKRLERYVDKVNDANPDLVLIAGDVITSTP
                     EYIETAGKYIGMIKSKYGTYSCVGDHDNWAYRRDYLRSLHEVEKALIDHNVEMPDNEL
                     RYIEIDSSRIGISFITHTYVSEISETTLQNIASSNKADFKIFLTHQPQQFLIDSAVKN
                     NYDLFLAGHTHGGQITLVFPFIHLTPTLVETKYIKGDYYFNKTLAIICGGLGMSLAPV
                     RYNSTPEIVLITLKNK"
     gene            complement(3201..>3893)
                     /gene="hemB"
                     /locus_tag="VF870_06055"
     CDS             complement(3201..>3893)
                     /gene="hemB"
                     /locus_tag="VF870_06055"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX9251785.1"
                     /translation="IIQRAVRALKAEVKNLLIITDVCMCEYTSHGHCGVLSGEEILND
                     ETVSLLAKEAVSHAEAGADIIAPSDMMDGRVAAIRKALDYKSFTKIPIMSYAAKYSSG
                     FYGPFRDAADSTPAFGDRKSHQMDIGNINEALREVAEDIEEGADIVMVKPAGPYLDVI
                     RAVKEKFGMPTAAYQVSGEYAMIKGAGANGWIDEERVMIESAFAIKRAGADMFLTYFA
                     KDLAKWIDKTRK"
BASE COUNT         1279 a          753 c          600 g         1261 t
ORIGIN      
        1 ccaatttcaa aaattggctt atcaggagca ttttttttgt ttgagttata ttttaacaac
       61 acaccatttt caccgcaggc aaacccggtg tattgatcaa caaattgaag atcataaaca
      121 actgtgctgt caaaaataaa ttgctctgtc cacgtatcgc cgccgtcagt tgaatttaaa
      181 aatttgtatc ctgcggcaga ccaaccgttt ttatcatcaa gaaaatcaat ggcaaatgat
      241 attccgaaaa agggtagatt tgtaaagctc cagttaacac cggcatctgt tgttctgata
      301 attccaagag gataaagtaa ctcgggatcg ccggataacg atatggcgtt aagcgaatca
      361 ataatataca catcataaat ttgatctggg cttaaaccaa tagacgacca actctctccg
      421 ccatcggtag ttttccaaac aactcccgca atatcaaccc tgccgccgca ggcatatcca
      481 aaagtcttac tgtaaaaaga aaatttattt attggcaacg ttgaaactcc acccggttca
      541 ataaatgaat ttaaccaggt taatccgcca tcagttgttt tttttattcc gttcccgccg
      601 ataaatccga cattttcatc aacaaaaaat attgtagaaa aaatttcatt ttgaaagaag
      661 acagaatcct taatccaatt attgccgcca tcagtcgttt ttaaaatgga ggttccaaac
      721 ggcggttcag atggataagt taatgcccag ccacgattag gattaataaa aaatatatct
      781 acgatagggg ttgttacggt gctgttttga atttcccagc tatttccgga gtcttttgta
      841 ttaattattt taccgccaag gctaacagcc caaccggttt tttcatttac aaaatagagt
      901 cttcttaacg tttcattaac cggtgattta agtttgtacc atccgtcagg ttcaggtact
      961 tgtgcaaaat attgattaga aaaaagaact aatacaattg cagaaatcaa aatatttgat
     1021 tttataatta acaaatacaa tcagtattta attattatgt ttttaactac aggaaattaa
     1081 gggcgttcta attaaaacca aaggaaagaa tatcttggtt aaaaaacact gtaacgattc
     1141 ataaatatat acagattaaa ataatattat tttttcacca acaaatattt tgccggtttt
     1201 gttacaaacc acattcattc cgaacataac tttattatta atatttctat attctgaaag
     1261 agtttttaac ggctcgttac ttctttctgc ggtatcctga tttgttgttg tgataacaca
     1321 tcttgcgcaa ggcttagctg ctttaaattc agcagcacct attctaaaat ctttccaacc
     1381 atcttcttca taaggattgc cgccgctaaa aacgaaattt gttctaaaac gattcatcgg
     1441 cacaggaatt gcaaatcttg aatttaaatc atccagagaa gattgaccaa taaccaaata
     1501 tggatacgca tctgcaaaac ttacaatatg ttcttcgtta atgtatttct tctcggccag
     1561 cctcttttca tcatcaggca tataaacaag tgaacacttt ttattaatcg cagaagagaa
     1621 ccaattatct aaatctttat ttacccggac agcattgcaa atgtcatccc aaatggtaac
     1681 tttaattact tctttcgaat cagactcaaa gggcacaatt atgtgtgagc catcttttgt
     1741 gtttaaaact ttaaacccgt gttcactaaa tgataatttt aacaaagcca ttagtggaaa
     1801 atcacgctga gtcataaaca tattgttttc atcaacgagt aaaaaacgcc gatcatattt
     1861 taatccacgt tcttctgcta ttgccgaatc aacacttatt ccgccaagag atttaatggg
     1921 ataaatataa atttcagaca gtgttagtat caatctattt attcttaagt gttattaaaa
     1981 caatctctgg tgtagagtta taccttaccg gtgcaagcga cattcctaaa ccgccgcaaa
     2041 tgatagcaag tgttttgtta aaataataat caccttttat atattttgtt tcaaccaaag
     2101 tgggcgtgag atgaataaaa gggaacacta atgtaatctg tcctccatgt gtgtgtcctg
     2161 caagaaagag atcataatta tttttaacag cactgtcaat taaaaattgc tgcggctggt
     2221 gtgttagaaa tattttaaaa tctgctttgt ttgatgatgc aatgttttga agggttgttt
     2281 cagaaatttc ggaaacgtat gtgtgtgtaa taaatgaaat tcctattctt gaagaatcaa
     2341 tttcaatata gcgcagttca ttatccggca tttcaacatt gtgatcaatt aatgcttttt
     2401 caacttcatg taagcttctt aaataatccc gcctgtatgc ccagttatca tggtcaccaa
     2461 cacaagagta agtcccatat ttagatttta tcattccaat atatttccct gcggtttcaa
     2521 tgtattccgg agttgaagta attacatctc ccgcaatcaa aaccaaatcg ggatttgcat
     2581 cgtttacttt atcaacataa cgctctaaac gcttttcgtc cgtgtatcta tctgcctgaa
     2641 gatctgaaat aaaagcaatc ttaaacccag ccagttcttt tggcagattt tgctttttat
     2701 agtccacgct acgtacgtct acagagttat aatcatatat gattcttgca ggaatataaa
     2761 cactaaaaaa cacaataagt gctaaaaata tttttacctg taaacgataa agtttttctt
     2821 tgtgtttttt ataaagctga taaaaaatca attttaataa atcaataaaa acgaaaagaa
     2881 cacttacctg cagtattaaa attattaaaa accaaaaagg ataaagcaac aaataatcgg
     2941 caaaaatgtt ttcaggaaat gcgacggatt cttttgttat cgcagcatat accgcattaa
     3001 agattaatat aacaggataa agattaagca cgacaagaaa agaatatata attatatatt
     3061 tcttctttct cggataatcg gggaaaaggg ttttaaagac acgatttact tttctggtaa
     3121 aatagtattc taataaagca agaaccacaa caccaattaa cacccggagt attatccaca
     3181 taaaattatt ctttcaacaa ttattttctt gttttatcaa tccattttgc aagatctttt
     3241 gcaaagtaag ttaaaaacat atctgcgccg gctcgtttta ttgcaaatgc agattcaatc
     3301 ataacccgct cttcatcaat ccagccattt gcgccggcac cttttatcat tgcatactct
     3361 ccgctaacct ggtaagcagc agttggcatt ccaaactttt ctttaaccgc tctgattaca
     3421 tctaaatagg gaccggcggg ttttaccatt acaatatctg caccttcttc aatgtcttct
     3481 gcaacttcac gtaaagcttc gttaatattt ccaatatcca tctggtgtga ttttctatcg
     3541 ccaaaagccg gagttgaatc tgcagcatct ctaaatggcc cataaaaacc ggaagaatat
     3601 tttgccgcgt aactcataat tggaattttt gtaaagcttt tatagtccaa agcttttcta
     3661 atagccgcaa ctcttccatc catcatatca gagggagcaa ttatatcagc gccggcttct
     3721 gcgtgtgata ccgcttcttt tgcaagcaga gaaactgttt catcatttaa aatttcttct
     3781 ccgcttaaaa ctccgcaatg tccgtgtgaa gtatattcgc acatacaaac atcagtaatt
     3841 attaataaat ttttcacttc ggcttttaat gcacggacgg cgcgttgtat aat
//
LOCUS       DASWBY010000322         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_499860, whole genome shotgun sequence.
ACCESSION   DASWBY010000322 DASWBY010000000
VERSION     DASWBY010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499860"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF870_06060"
     CDS             complement(<1..256)
                     /locus_tag="VF870_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251786.1"
                     /translation="MKKVLFISYFWPPSGKASLHWPLDIIRHLPKYEIEPIILTVEEE
                     TFTQKDESLLSKVDLTWKVIKSKAFEPFDLYRIFTGKKKNE"
     gene            416..811
                     /locus_tag="VF870_06065"
     CDS             416..811
                     /locus_tag="VF870_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251787.1"
                     /translation="MLIDIATASPPFKVEQSFAASELKKRMGGTAAISRMIDMVANQS
                     GIGHRFFVIKDGEENSAEKFFTKDGSHFSPDTKTRMIEYEKWSISLTKQAVSELAEKN
                     KIDFEKIDRLITISCTGFFAPGLDYELIK"
     gene            818..>1534
                     /locus_tag="VF870_06070"
     CDS             818..>1534
                     /locus_tag="VF870_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251788.1"
                     /translation="MSPNVKRTNIGFMGCAASLVGINSVWEAMQQQGNENILMVSVEL
                     CSLHLQTEPTRDNILANMIFADGAAAAYFSKNEMYDNRKLEIQFAESFLFEDSAKFMG
                     WKIGNNGFEMMLSSELPKIILNSAAPRAKEILAEKRIDVSKIKHWALHPGGRAILDSL
                     QNGLELSDEQLKASREVLLKFGNLSSVSILFVLKNILMVSVELCSLHLQTEPTRDNIL
                     ANMIFADGAAAAYFSKNEMYD"
BASE COUNT          490 a          244 c          299 g          501 t
ORIGIN      
        1 tttcgttctt ctttttccct gtgaatattc tgtacaggtc aaacggttca aaagctttcg
       61 atttaattac cttccaggtt agatcaactt tgcttaaaag actctcatct ttttgagtaa
      121 aagtttcttc ttcaactgtt aggatgatcg gctcaatttc atattttggt aaatgtcgga
      181 ttatatctaa cggccagtgg agcgaagctt tgcctgatgg cggccaaaaa tatgagatga
      241 acaaaacttt tttcatttgg atgtaaagaa attattatca cttataataa acagccggaa
      301 atctaacttc attttacttt ctaataaaat atctgctcaa atttatataa gtaatatgga
      361 atttgaaacg aatcttcgat tctttatgtt aatatgtaaa ctatttagag gaaatatgtt
      421 aatcgatatc gcaacagcgt cgccgccatt taaggttgag caaagttttg ctgcaagtga
      481 actaaaaaaa cgtatggggg ggaccgctgc tatcagcaga atgattgata tggtggccaa
      541 tcaatcagga attggccatc gattttttgt aataaaagat ggtgaagaaa attctgcaga
      601 gaagtttttt acaaaagatg gatcgcattt ttcaccggac acaaaaacgc gaatgattga
      661 atatgaaaaa tggtctatct ctttgacaaa gcaggctgta agtgaattag cagaaaaaaa
      721 taaaatagat tttgaaaaga ttgatagatt aattacaatt tcttgtacgg gattttttgc
      781 cccaggtctg gattacgaac tgattaaata atttaatatt tctccaaacg ttaaaagaac
      841 aaatattggt tttatgggat gtgctgcttc tttggttgga attaattccg tttgggaagc
      901 gatgcaacag cagggaaatg aaaacatttt aatggtctcc gttgagcttt gcagtttgca
      961 tttacaaact gaaccaacta gagataatat tcttgcgaat atgatttttg ctgatggtgc
     1021 cgccgccgct tacttttcga aaaatgaaat gtacgataac cgaaaactgg aaatacaatt
     1081 tgctgaatct tttttgtttg aagattctgc aaagtttatg ggatggaaaa tcggcaacaa
     1141 tggttttgaa atgatgcttt cttctgagct gcccaaaata attcttaatt cggcagcacc
     1201 tagggcaaaa gaaattttag ctgagaaaag aattgatgta tctaaaataa aacactgggc
     1261 acttcatccg ggcggcagag caattttaga ttcattgcaa aacggacttg aactttctga
     1321 tgagcaatta aaagcatcgc gggaagtgct actaaaattt ggaaatcttt cttctgtttc
     1381 catccttttt gttcttaaaa acattttaat ggtctcggtt gagctttgca gtttgcattt
     1441 acaaactgag ccaacccgag ataatattct tgcgaatatg atttttgctg atggtgccgc
     1501 cgccgcttac ttttcgaaaa atgaaatgta cgat
//
LOCUS       DASWBY010000323         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_725914, whole genome shotgun sequence.
ACCESSION   DASWBY010000323 DASWBY010000000
VERSION     DASWBY010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725914"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VF870_06075"
     CDS             <1..1151
                     /locus_tag="VF870_06075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9251789.1"
                     /translation="TVEEETFTQKDESLLSKVDLTWKVIKSKAFEPFDLYRIFTGKKK
                     NEKLVSSETISTENTSLTHRIALWIRLNLFVPDARVGWNFSAIKASSTFIKKEKIDVI
                     VSIGPPHSSHLIGLRLSKKYSITHIPVFIDPWVDIVYYKNLKRSALTIKLDNHFEKSV
                     LENAKQVVFVNKSTEEDYLNKYNFLKNKTNVLYWGYDEDDFNDLQIEKIKKDEKIIVH
                     AGNMFAYQNPKNFWKQIKIENEKGNRTIIRFVGSVDKEILDYLYSIGFKENVQLAGFL
                     PYKEMIREILRADILLVCSTEPRHVPGKLFEALRTGNPIIAFGDDNDEVKKIIEEANA
                     GMMFRYNESGAEFLKGFNKIKPDQNFGKKYDRAQISKEFYTILMKLYN"
     gene            1162..>1652
                     /locus_tag="VF870_06080"
     CDS             1162..>1652
                     /locus_tag="VF870_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251790.1"
                     /translation="MSKFYQILSLPYLYIRCNFFWWKADKTNLPGEHFFKFGKLVGLK
                     LLQKFILSPKLLLNPVSIVRFFEYDYTLRSYSAHNSRRDKILDISSPYLFGFYLASFF
                     EGEYNYRNPDSNDLTLVKKYSSKIKFKMQYSANSEDATKLTFSNNSFRYIISISVIEH
                     ING"
BASE COUNT          616 a          218 c          295 g          523 t
ORIGIN      
        1 taacagttga agaagaaact tttactcaaa aagatgagag tcttttaagc aaagttgatc
       61 taacctggaa ggtaattaaa tcgaaagctt ttgaaccgtt tgacctgtac agaatattca
      121 cagggaaaaa gaagaacgaa aaacttgtat catcagaaac gatttcaaca gaaaatacta
      181 gtttaactca tcgcattgct ttgtggataa gattgaacct gttcgttcca gatgcaagag
      241 taggttggaa tttttctgcg attaaagcca gtagcacatt tattaaaaaa gaaaaaattg
      301 atgtcattgt atcaattggt ccaccacata gctctcatct gattggatta agactaagca
      361 aaaaatattc cattacgcac atacctgttt ttattgatcc gtgggttgat atagtttatt
      421 acaaaaatct taaaagaagt gcattaacaa ttaaacttga taatcacttt gaaaagtctg
      481 tattagaaaa tgcaaagcag gttgtgtttg taaataaatc caccgaagaa gattatttga
      541 ataaatataa ttttcttaaa aataaaacca atgttttgta ttggggatat gatgaagatg
      601 actttaacga tttgcaaatt gaaaagatta agaaagatga aaaaattatt gttcatgcag
      661 gaaacatgtt tgcttaccag aatccaaaaa acttttggaa gcagataaaa attgagaacg
      721 agaaagggaa tagaacaata attcgttttg tgggttcagt ggataaagaa atattggatt
      781 acctttattc tataggattt aaagaaaatg ttcaattagc gggttttctt ccttacaagg
      841 aaatgataag agaaattctt cgggctgata ttttattagt ctgctcaaca gaaccacgcc
      901 atgttcctgg taaactattc gaagcattac gaacaggaaa tccaataata gcttttggcg
      961 atgataatga tgaagttaaa aagataatcg aagaagcaaa tgctggcatg atgtttagat
     1021 ataatgaaag tggtgcggag tttttaaagg gttttaataa aataaaaccc gatcaaaatt
     1081 ttggcaaaaa atatgaccgg gctcaaataa gtaaagagtt ttatactata ctaatgaagc
     1141 tatataatta atgaaagcaa aatgagtaag ttctatcaaa tattatcgct tccttattta
     1201 tatattagat gtaatttctt ttggtggaaa gcagataaaa ctaatctgcc tggtgagcat
     1261 ttctttaagt ttggaaaatt ggttggatta aaactgctgc aaaagtttat tctatcgcct
     1321 aaacttctgc ttaacccggt ctctattgta agattttttg agtacgatta tacactaaga
     1381 agttattctg ctcataattc aaggagagat aagatactgg atatttcatc gccatatttg
     1441 tttggatttt atttggcgag tttttttgag ggagaataca actacagaaa tccagatagt
     1501 aatgatttaa ctctggtaaa aaagtattcc tctaaaataa aatttaagat gcaatattca
     1561 gccaattcag aagatgcaac aaagttaact ttttcaaaca attcatttag atatatcatt
     1621 tcaatcagcg tgatcgagca tataaatggt aa
//
LOCUS       DASWBY010000324         4098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_87690, whole genome shotgun sequence.
ACCESSION   DASWBY010000324 DASWBY010000000
VERSION     DASWBY010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87690"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VF870_06085"
     CDS             <1..1572
                     /locus_tag="VF870_06085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9251791.1"
                     /translation="QECNEQKDKAKEESKVKAEIFTELMFRHWNDWRGHKRSHLFLLD
                     VATGNFKDLTEGNKEDVPPLALGSSNDYNFSPDGSEVAYALNPEFTKATSTNIEIYLA
                     GLTSPNAPKIISTSKGVDCQPVYSPDGKWLAWTSMKRAGFEADKKDLILFERKTGKMK
                     NLTEDYDRSVDEFVWAPDSKTIYFSAANEIYNSIYKLDVEEEEIEIFYKENYNTNIQL
                     SKDGKTLFFLKQRTDLPAEIFSLSTDGKNTLKRITFTNEEILSQLEMNPVETFWCDGA
                     NGDKVESILIKPPFFDPNKKYPMMFLIHGGPQGAWEDNFHYRWNLQMFAGAGYVVVAP
                     NPRGSTGYGQKFTDEISGDWGGKVYTDLMNSYDYALKNYSYIDPKNTFASGASYGGYM
                     INWIEGHTDRFSALFCHNGTFNLESMWGTTEELWFPEWDVNGTPWENREAYVKWSPHQ
                     YAKNFKTPMLIVHSAFDFRLSEEQAFQAFTTLQRLGVESKFLYFPDETHFVSKPQNAK
                     LWWNTVFDWFKSHQK"
     gene            1581..1997
                     /locus_tag="VF870_06090"
     CDS             1581..1997
                     /locus_tag="VF870_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX9251792.1"
                     /translation="MKERDLEIVTPDDEIFENLGDLGAFRTGGKTERELEEDYTERSK
                     YVEENLWSKVQRVGKKISFAKDIVALYNYMKDPYVSWHRKAIVVMGLAYFISPIDTIP
                     DIAPLIGYLDDLGVITAVIKFLGSELIPYYDSKYRR"
     gene            2089..2640
                     /locus_tag="VF870_06095"
     CDS             2089..2640
                     /locus_tag="VF870_06095"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9251793.1"
                     /translation="MTILKTMNYKLLKSEIRYKGKVFDHQVDEIEYESGNKAIREIAI
                     HPGGAVVVPVKDDGKIILVKQFRYPLQKTLIELPAGKLDKGEDPLVCATRELEEETGY
                     KAKEVKKLGEIYTAPGYCTEILHIYYASGLIPGNHNREEGERGMEILEFSLNEIEKMI
                     SSGEITDAKTIVGIHYLKNLNNH"
     gene            2703..3461
                     /locus_tag="VF870_06100"
     CDS             2703..3461
                     /locus_tag="VF870_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HEX9251794.1"
                     /translation="MKTYNHLLNTIKEKGAAYLVLLDPDKLPENKLAKFLKHCEKSGV
                     DGFLVGGSLMVNGDFETFIEKVKVNTNLPSIIFPGGINQVSSFADAILFLSVVSGRNP
                     EHLIGKHVLAAPSIKKSGIEPISTAYILVESGSTTTAIYMSGSLPIPRNKPEIAAATA
                     LASEYLGMKLIYLEAGSGADNSVPNEMVKAVSAQCSIPVIVGGGIRNPRTAREKVESG
                     ASIIVTGNYFEDENNWDLIKDFASAVHIKQGILV"
     gene            3569..>4098
                     /locus_tag="VF870_06105"
     CDS             3569..>4098
                     /locus_tag="VF870_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX9251795.1"
                     /translation="MDAQKFIIDSLNESSETKLKIKDQLSDEIIKAVDILVASYKDGN
                     KLLLCGNGGSAADCQHIATEFMIRLSHHIQRPALPAIALTTDSSNLTAGGNDIGFENV
                     FARNVEGLGVLGDVLLAISTSGNSPNILKAVEAAHAKGMKVIGLLGGTGGKLKSLVDL
                     PIVVPSSNTQRIQEGH"
BASE COUNT         1407 a          660 c          840 g         1191 t
ORIGIN      
        1 caggaatgca atgaacaaaa agataaagcc aaagaagaaa gcaaagtaaa ggcagagatc
       61 ttcactgaat taatgttccg tcactggaat gattggcgcg gacataagcg cagtcatttg
      121 tttttgcttg atgttgcgac cggaaacttt aaagatctta cagaaggaaa caaagaagat
      181 gttccgcctc ttgcacttgg gtcatcaaac gattacaatt tttctcccga tggaagtgaa
      241 gttgcatatg cattgaatcc tgaatttaca aaagcaacaa gcactaatat tgaaatttat
      301 ttagctggtt taacttctcc taatgctccc aaaattattt ctacaagtaa aggtgttgat
      361 tgtcagccgg tatattctcc tgatggaaaa tggcttgcat ggacttctat gaaacgcgca
      421 ggatttgagg ctgataaaaa agatttaata ctttttgaaa gaaaaaccgg taagatgaaa
      481 aatcttactg aagattatga ccgatcagtt gatgaatttg tgtgggctcc ggattcaaaa
      541 acaatatatt tctcagcagc taatgaaatt tacaattcaa tttataagct tgatgttgaa
      601 gaagaagaaa ttgagatttt ctacaaagaa aattataaca caaatattca gttgtcaaaa
      661 gacggcaaaa cacttttctt cttaaaacaa agaacagatt tacctgcaga aatattttct
      721 ttaagtacgg atggcaaaaa cactttaaag agaattactt ttacaaatga agaaatactt
      781 tcacaacttg aaatgaatcc tgtagaaact ttctggtgcg atggtgcaaa cggagataag
      841 gttgaatcta tcctaatcaa accccccttc tttgatccaa ataaaaaata cccaatgatg
      901 tttttaattc acggtggacc gcagggagcg tgggaagata attttcatta cagatggaat
      961 cttcaaatgt ttgctggtgc aggatacgta gttgttgcac ccaatccaag aggctcaacg
     1021 ggatatgggc aaaaatttac agatgaaata tcgggtgatt ggggcggtaa agtttataca
     1081 gatttgatga acagttacga ttacgcttta aagaattatt cttacatcga tcctaaaaat
     1141 acttttgcat caggtgcttc ttatggcgga tacatgatta actggattga aggacatact
     1201 gatagattta gtgcattgtt ctgccacaac ggaactttta atcttgaaag catgtgggga
     1261 actactgaag aattatggtt tcccgaatgg gatgtaaatg gaactccctg ggaaaatcgt
     1321 gaagcttatg taaaatggtc gccgcatcaa tatgccaaga attttaaaac accaatgcta
     1381 atcgtgcata gtgcatttga ttttagatta tccgaagagc aggcttttca ggcatttaca
     1441 acactgcaaa ggctgggagt tgaaagtaag ttcctttatt ttcctgatga aacgcatttt
     1501 gttagtaaac cacaaaacgc aaagttatgg tggaacacag tatttgattg gtttaaatca
     1561 catcaaaaat aggagaggtg atgaaagaaa gagatttaga aatagttaca cccgatgatg
     1621 aaatttttga aaaccttggt gatcttgggg cttttagaac gggtggaaaa actgagcgtg
     1681 aattggaaga agattacact gaaagaagca aatacgttga agaaaatttg tggagtaaag
     1741 ttcagcgtgt aggaaagaaa atttcttttg caaaagatat agttgctctt tacaattata
     1801 tgaaggatcc ttacgtaagc tggcatcgta aagcaattgt tgtaatggga ttggcttatt
     1861 ttatctcgcc gattgatacc atcccggata tagcgccgct tattggatat cttgatgatc
     1921 ttggggttat tactgcagta attaagtttc ttggcagtga gcttattcca tattacgatt
     1981 ctaaatacag aaggtaatat ttaatcatca ttgcgagtgc tgttttaagg acgaaacaat
     2041 tttatactta aaatttataa aattacttca ctagaagact cgttcgcaat gacaatcctt
     2101 aaaactatga attataaact cctaaaatca gaaatacgtt ataaaggcaa agtatttgat
     2161 catcaggttg atgagattga atacgaaagc ggtaataaag caattagaga aattgcaatt
     2221 catccgggcg gagcggttgt tgttcccgtt aaagatgatg gaaaaataat tttagtaaag
     2281 caattccgat atccacttca gaaaacattg attgaacttc ctgcaggaaa acttgataaa
     2341 ggtgaagacc cattagtttg tgcgacccgc gaacttgaag aggaaacagg ttacaaagca
     2401 aaggaagtaa aaaaactcgg tgagatttat acagcgccag gttattgtac ggaaattctt
     2461 catatttatt atgcaagcgg attaattccc ggaaatcata atcgtgaaga aggcgaacgt
     2521 ggtatggaaa tacttgagtt tagcttaaat gagatcgaga aaatgatttc aagcggcgaa
     2581 ataactgatg ctaaaactat tgttgggata cattatttaa agaatctgaa taatcattga
     2641 aaatccattc ttttttctca aaacctaaat ttctatattg aggcagtata taatcaagaa
     2701 ttatgaagac ttataatcat ctgctaaata ctatcaagga aaaaggagcg gcatatttag
     2761 ttctgctcga tcctgacaaa cttcctgaaa ataaactcgc taaattttta aaacactgtg
     2821 agaaatccgg agttgatgga tttttggtcg gtggcagttt aatggttaat ggtgattttg
     2881 aaactttcat tgaaaaagta aaagttaata caaatcttcc ttcaataatt ttccctggcg
     2941 gtattaatca ggtttcctct tttgcagatg ccattttatt tctttcagtt gttagcggaa
     3001 gaaacccgga acatttaatc ggtaaacacg ttcttgccgc accatcgata aagaaaagcg
     3061 ggatagaacc aatttcaaca gcttacattt tagttgaatc aggttcaaca acaacagcaa
     3121 tttatatgag cggaagttta ccgataccaa gaaacaaacc tgaaattgct gcagctactg
     3181 cgcttgcatc agaatatctt ggaatgaagt tgatttatct tgaagccgga agcggtgcag
     3241 ataattcagt cccgaatgaa atggttaaag ctgtgagtgc acaatgttcc attccggtaa
     3301 ttgttggcgg tggaataaga aacccgagaa cagcaagaga aaaagttgaa agcggtgcat
     3361 cgatcattgt aaccggtaat tattttgagg atgaaaacaa ctgggattta attaaagatt
     3421 ttgcttctgc tgtgcacatt aaacaaggta ttttagtata gcaataaaag tcattccggg
     3481 tcgccaaaag ggaagaagga atccagagag attgcttcat cagtaaaaac tttcctcgca
     3541 atgacaatca cacaataagg ataaaaaatt ggacgctcaa aaatttataa tagattcact
     3601 gaacgaaagt tctgaaacaa agcttaaaat taaagatcag ttaagtgatg aaataattaa
     3661 agctgttgat atccttgttg cttcttataa agacggaaac aaacttttat tatgcggtaa
     3721 tggcggcagc gctgcggatt gccagcacat tgcaactgag tttatgataa gattaagtca
     3781 tcatattcag cgacccgctt tgcctgcaat tgctcttaca acagattcat caaatcttac
     3841 cgcaggggga aatgacattg gttttgaaaa tgtttttgca cgaaatgttg aaggacttgg
     3901 tgttcttggg gatgttcttc tagcaatttc tacaagcggc aattcaccaa acatcctaaa
     3961 ggctgttgaa gcagcgcacg caaaaggaat gaaagtgatt ggcttgcttg gtggcacagg
     4021 cggtaaacta aaatcacttg ttgatctgcc gattgttgtc ccttcatcca acacccagag
     4081 aatacaggaa ggacacat
//
LOCUS       DASWBY010000325         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_48917, whole genome shotgun sequence.
ACCESSION   DASWBY010000325 DASWBY010000000
VERSION     DASWBY010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48917"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VF870_06110"
     CDS             complement(<1..613)
                     /locus_tag="VF870_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9251796.1"
                     /translation="MNPYKGLKGLPHDMWALFFTSLINRSGTMVIPFLALYLTKKIGV
                     SPAEAGTALLVYGAAAFIAAPLTGKLSDKLGSLKVMKFALYGSGVLFFAYSFITNYNW
                     ILVATFILAAVNESFRPANLSMITEIVAPPQRRMAFALNRLAINAGMSIGPVIGGFLT
                     LIDFHYLFYANAVASIAAGIYFSSIKWSSLAGSEKEKTTEEVSK"
     gene            complement(785..2545)
                     /locus_tag="VF870_06115"
     CDS             complement(785..2545)
                     /locus_tag="VF870_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251797.1"
                     /translation="MKIFTIFFSILLFSTLMAQSQPQISFNDLLISSEENPQMKLQAR
                     EIALKNNLPWSIYLSDKIFIEALGVENEKPVYAVITNLLNPYEGGSTAFFEEIGSKYD
                     LASARINYGNGKIINPTLGYPEIIIPTEAVSFLLFSESTNDKVWSINATTGDVINPDY
                     IPTDPVNLNTPTQARLSPGGKITISDQNNDGVFAYDTLGVPSGIFAPAGGVNLNILDN
                     VRGHNYRPNGNLLVTVAGSANSNSIAEFDGLGNYLGQFITAGSGGLNSPWDILFRTND
                     VLISASSSDAIHRYDLTGAYLDNFYQAGTTSYFPEQMQELSNSNIAVADFGVGGGVKI
                     YSNTGTPLTTLGVVTANRGVYGLPNGNFLTTNAAGLFEINGTTGDTVRQIIAGVSGRF
                     ITPYDLAIIPVELTSFVGSSSNGRVELSWTTATELNNSGFEIQRSSDGYNYIKIGFVP
                     GNGTTSEPKAYNFIDNNSGIGKQFYRLRQIDFDGTFNYSGIVEVDVAAPIKYLLEQNY
                     PNPFNPSTMISYSIPQNSFVTLKVYDIIGNEVATLVNQTQSAGKYDIRFDASNLSNGV
                     YLYTIKTDNFSSTKKMILMK"
     gene            complement(2659..>2960)
                     /locus_tag="VF870_06120"
     CDS             complement(2659..>2960)
                     /locus_tag="VF870_06120"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX9251798.1"
                     /translation="NQLKINSAVSNAKAFIAVRKEFGTFDKYIWSFVNGKPIKNNFKS
                     LKELPARTELSDFISKDLKKRGFNFVGSTIIYAHMQATGMVNDHLTSCFRYNELK"
BASE COUNT          977 a          541 c          491 g          951 t
ORIGIN      
        1 tttttgaaac ctcttctgtg gttttttctt tttcactacc agctaaagag gaccatttaa
       61 ttgaactgaa atatattcca gcagcaattg atgcaacagc atttgcataa aacagataat
      121 gaaaatcaat taatgtaaga aagccgccaa taacaggacc aatactcatt cctgcattta
      181 tcgcaagtcg gtttaacgca aaagccattc tgcgctgcgg tggagctacg atttcggtaa
      241 tcattgaaag gtttgctggc cggaatgact cgtttactgc ggcaagaata aaagttgcta
      301 caagtatcca attgtagttt gttataaaag agtaagcaaa gaagagaaca cctgaaccat
      361 acaaagcaaa cttcattact tttaaggaac caagtttatc agaaagtttt cccgttaacg
      421 gtgcagcaat aaaagcggcg gcaccataaa ccagcaatgc tgttcctgct tctgcgggac
      481 tgacgccaat tttttttgta agataaaggg ctagaaaagg aataaccatt gttccggatc
      541 gattgatgag cgaagtaaag aacagcgccc acatatcatg cggaaggcct tttaatcctt
      601 tgtatggatt cataaaaaat atttgcttaa ttattttttt agtcgcggaa agttaagaaa
      661 agttgtttac ttatttaaaa taaaaaaggc acgattactc gtgccttcat tattttgtat
      721 tctggaaatt cgttcctgaa taattatgaa agtaaacgca cgaaattatt cgtgcgccac
      781 ataattattt catcagtatc atcttcttgg tagaagaaaa attatcagtc ttgatcgtat
      841 acaaataaac accgttgctt aaattgcttg catcaaatct aatgtcatac tttccagcag
      901 actgagtttg attaacaaga gtagcgactt cattaccaat aatgtcataa actttaagag
      961 ttacaaaaga attttgtgga atactgtaac taatcattgt tgatggatta aatggatttg
     1021 gataattctg ttctaataaa tatttaatcg gggccgcaac atcaacttca actatacccg
     1081 agtaattgaa tgtcccatca aaatcaattt gtcttaagcg ataaaattgt tttccaattc
     1141 cggagttatt atctataaaa ttataagctt tcggttctga agttgttcca ttaccgggaa
     1201 caaatccgat tttaatataa ttgtatccat cggaacttct ttgtatttca aatcctgaat
     1261 tatttaattc agttgcagta gtccagctta gttcaaccct tccattacta cttgagccaa
     1321 caaacgaagt tagctcaacc ggaataattg caagatcata aggggttata aatcttccgg
     1381 aaactcctgc aataatttgt cttacagtgt caccggttgt tccgtttatt tcaaaaagtc
     1441 ctgctgcatt tgtagttaaa aaatttccat ttggtagtcc ataaacacct ctgtttgctg
     1501 ttacaacccc aagtgttgta agtggtgttc cggtatttga atatattttt acaccaccgc
     1561 ccacaccgaa atctgcgaca gcaatattag agtttgataa ctcctgcatt tgctcaggaa
     1621 aataacttgt agtacccgcc tgataaaaat tatctaaata agctccggta agatcatatc
     1681 tatgtatcgc atcgcttgat gaagcgctga taagaacatc attagtgcga aataaaatat
     1741 cccatggact atttaatccg ccagaacctg ccgtaataaa ctgacccaaa taattaccca
     1801 gtccgtcaaa ttcagcaata gaatttgaat tagcactacc tgcaactgta actaaaagat
     1861 taccattcgg tctataatta tgccctctta cattatctaa aatatttaga tttacacctc
     1921 cggcgggagc aaaaattccg gatggaacgc caagtgtatc ataagcaaaa acaccatcat
     1981 tattttgatc tgaaatagtg attttgccgc caggagatag tcttgcctga gttggtgtat
     2041 ttaagttaac aggatcagta ggaatgtagt ctggatttat aacatcaccc gttgtagcat
     2101 tgattgacca aaccttatca ttggttgatt ccgaaaacaa taaaaagctt acagcttctg
     2161 ttggaatgat aatctcagga tagccaagtg tgggatttat aatctttcca ttgccataat
     2221 tgattcgcgc agaagctaaa tcatatttag atccaatttc ttcaaaaaag gcagtgcttc
     2281 caccttcata aggattgaga aggtttgtaa ttacagcata aacaggcttc tcattttcta
     2341 ctccaagagc ttcaataaaa attttatctg ataaatagat actccaggga agattattct
     2401 ttaaagcaat ttctcttgct tgtaatttca tttggggatt ttcttcagat gaaattagta
     2461 aatcattgaa gctaatttgc ggctgactct gagccattaa ggttgagaaa agcaaaattg
     2521 aaaagaaaat agtaaaaatt ttcataccgg gcctcttatg tttattcttg aaatagttgt
     2581 tgtgcaaaac tcaattttca aacttatttc aatatgagca taaatgcact ataagtcaat
     2641 cggtgtaaag aaatattttt attttaactc gttatatcta aaacaacttg taagatgatc
     2701 attaaccatt cctgttgcct gcatatgcgc ataaataatt gttgaaccaa caaagttgaa
     2761 tccgcgtttc ttcaaatctt tacttataaa atctgaaagt tctgtacgag ctggaagctc
     2821 ttttaaagat ttaaaattat tcttaatcgg tttcccattt acaaaactcc agatatattt
     2881 atcaaaggtt ccaaattctt ttctaacagc aataaatgct tttgcattac taaccgcaga
     2941 atttatcttt aattgattac
//
LOCUS       DASWBY010000326         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_127102, whole genome shotgun sequence.
ACCESSION   DASWBY010000326 DASWBY010000000
VERSION     DASWBY010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127102"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1892
                     /locus_tag="VF870_06125"
     CDS             <1..>1892
                     /locus_tag="VF870_06125"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX9251799.1"
                     /translation="VSTYNGLNRLNPNKLEEGFHLFFASDTNKNSISDNLVWRVSQSI
                     NDKNILWICTANGLCKYEKDKDEFHRLPINPNIRLQFSKSFSWVAEQNVDGNNILWAA
                     TYGGLFRIDLNNNNQDQFVSDKKNLSGLLGNQIDQLLIDRSGVLWIGTDKGLNYYSSK
                     AQKFNKIFTQNINDQTSQELLNADVKAIINNGNNEFFIAAYDALFKLSVKDRNTEIKK
                     FNEFNNLNLWSLEKGNNNDLWIGTYGNGLIHYDLTKSQKRVINIESPVFKSSAFKFIK
                     ALLLSNDGLLWIGFWGGGLACLDTKTEKYQIWIHDESNPKSLSFNDVWTLYEDKLGRL
                     WIGTNGGGLNLYDPKNNGEFINWKFDANNSHSIINNAVMAINETPSKNSNETILLIGT
                     ENGLCKAIVKNSDSNKYDVDLSFDNYFDTDALNDRSISGMLSDDNELLWISSQFGLLR
                     FNTADKSLAVFGFSDGLQSNIFNSNAYCKSESGLMLFGSVKGPVIFNPSKINLSEYEP
                     NIIFTDFQIFNESIDPGEDSPLKTSIAVSNNINLSYDQNTITFNFASLDFNSSEHIHF
                     LYKLEGSDKDWIYSIKRRFATYNNLNPGTYYFRLKATNSDGIWGNTVKSIKVTINPPW
                     WKTGWA"
BASE COUNT          691 a          248 c          330 g          623 t
ORIGIN      
        1 gtaagcactt acaatggttt gaatagatta aatcctaata aacttgaaga aggttttcat
       61 ttattcttcg ctagtgatac aaataaaaat tcaataagcg acaatctggt ttggcgggtt
      121 agccaatcaa ttaatgataa aaatatttta tggatttgca cagcaaatgg cttatgcaaa
      181 tatgaaaaag ataaagatga attccatagg cttccaataa atccaaatat tcgcttacaa
      241 ttcagtaaaa gtttttcctg ggtagcagag cagaatgttg atggcaataa tattctttgg
      301 gctgctactt atggcggttt gttcagaata gatttgaata ataataatca ggatcaattt
      361 gtaagtgata agaaaaactt aagcggactt cttggtaatc agatagatca attattgatt
      421 gatcgttccg gagttttatg gatagggact gataaaggat tgaactatta ctcatcaaaa
      481 gcccaaaagt ttaataaaat atttacacaa aatattaatg atcaaacatc gcaggaatta
      541 ttaaatgcag atgtaaaagc aatcataaat aatggtaata atgaattttt tattgcagca
      601 tatgacgccc tatttaaatt aagtgttaaa gatcggaaca cagaaataaa aaaattcaac
      661 gaatttaata accttaatct ttggtcgttg gaaaaaggca acaataatga tttatggata
      721 ggcacttacg gcaatggttt aattcactat gatcttacta aatcacaaaa aagagtcatc
      781 aatattgaaa gccctgtttt caaaagttca gcatttaaat ttataaaggc tttactttta
      841 agtaatgatg ggttgctttg gattggtttt tggggcggcg gtttagcctg cttagataca
      901 aaaacagaaa aatatcaaat ctggattcat gatgagagta atcctaaaag tttaagtttt
      961 aatgatgttt ggactttgta tgaagacaaa ttaggcagat tgtggattgg aactaatgga
     1021 gggggtttga acctttatga tcctaagaat aatggtgaat tcattaattg gaaatttgat
     1081 gcaaataatt cacattcaat tatcaacaac gctgtaatgg caataaatga aaccccatca
     1141 aaaaacagta atgaaactat acttctaatc ggaactgaga atggattatg caaagcaata
     1201 gttaagaaca gtgattccaa taagtatgat gttgatttaa gctttgataa ttatttcgac
     1261 actgatgcat taaatgacag atctattagc ggaatgttga gtgatgataa tgaattatta
     1321 tggataagta gtcaatttgg ccttttaagg tttaatacag cagataaatc attagctgtt
     1381 tttggatttt ccgatggttt acaaagcaat atttttaatt ctaatgccta ctgtaaatca
     1441 gagagtggtt tgatgctttt tggatctgta aagggaccgg ttatttttaa tccttcaaag
     1501 atcaatcttt ctgaatatga accaaatatt atatttacag attttcaaat ctttaatgaa
     1561 tcaattgatc ccggagaaga ttcaccatta aaaacatcta ttgcagtttc aaacaatatt
     1621 aatctttcat acgatcagaa tactataaca tttaattttg cttcattaga ttttaattca
     1681 tccgaacata ttcatttttt atataaactt gaaggatcag ataaagattg gatttattcc
     1741 attaaaagaa gatttgcaac atataacaat ctaaatcctg gcacatatta ttttagatta
     1801 aaagctacga acagcgatgg tatttggggt aatactgtta aatcaataaa ggttacaata
     1861 aatccacctt ggtggaagac tggctgggct ta
//
LOCUS       DASWBY010000327         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_238069, whole genome shotgun sequence.
ACCESSION   DASWBY010000327 DASWBY010000000
VERSION     DASWBY010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238069"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VF870_06130"
     CDS             <1..567
                     /locus_tag="VF870_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9251800.1"
                     /translation="GGRYITYIQASAASTDQIDDAQEELKTIMREAHHLYPGQDDDFT
                     VRNQAELTEAATATSKILTILLASVAGVSLIVGGIGIMNIMLVSVTERTREIGIRLSV
                     GARTSDIMIQFLTEAIVLSLAGGLIGILLSIGIIFILNNYTSMAAVINPQIIFIAVSF
                     AGVIGIFFGFYPAKKAANLNPIDALRYE"
     gene            590..1915
                     /locus_tag="VF870_06135"
     CDS             590..1915
                     /locus_tag="VF870_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9251801.1"
                     /translation="MKTSYYFIFIIFCLSVFNNSYSQKLLTLDDAVTIALNQNTNLVK
                     STNSLATFQAGVKNAYGNLIPTLSLGGNFGWQRVTNSERTTQLDSLGNQITTGPTQYD
                     SRFWSVSAGGSVTLFNGLSNLAQISKSKSDFESAKLTLEKLKQDIVLQAITLYTQIIS
                     YQKLLNFQEENLKYNEGLLAQIKEKFDLKMVPISDVYSQEAQTANSKVFYLQAKNNLE
                     IAKINLLNYLSIDVSQNYQFDSTAANIKDSTLIKSNIDSLYQYALNNRKDYQSMLFEL
                     ESSQHQLTIARSGLFPNLSANYGFGSSFNDPANIFNQKVYSLGLSLNIPIFSNWSTEN
                     AIQSADVQIQNSNEDLKVLELSIKSQVKTANLNLETTKQQLDASETALTASRESWKIE
                     TETYNLGAATYLDLQLSYNNYLQAQYNKISNEFSYIISQYTLLNALGNY"
     gene            1954..>2817
                     /locus_tag="VF870_06140"
     CDS             1954..>2817
                     /locus_tag="VF870_06140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9251802.1"
                     /translation="MNKLINKISKMNRTSLIGSSILLLCFIGFIDWSTGTELSISIFY
                     LIPIAICAWFVNKWTGVVLAIISITIWFLSDYFDSSSYNHPITPYWNALIMLGIFVLV
                     SELLSALKRVIDRENILALNIQKNLLPGKNPEISDYKVFSIWEPTRVVSGDYYDFIQL
                     KENVLGISLADVCGHGFPAALLMSNVQATFRIIAGHNHAPKEVCDHLNSILISYNMPD
                     KFTSFFYGILDAENKEFTYSNAGHPPPIILRANGKIDRLSVGGFLLGVNPIAKYEQET
                     VYIESGDIILLY"
BASE COUNT          945 a          439 c          496 g          937 t
ORIGIN      
        1 ggtggaagat atataacata catacaggct agtgctgcat caactgatca gatagacgat
       61 gcacaggaag aacttaaaac tattatgaga gaagcacatc atctgtatcc aggacaggat
      121 gatgatttta cggttagaaa ccaggcagag ttaactgaag ctgcgaccgc aacatccaag
      181 atccttacta ttcttcttgc atctgtcgcc ggtgtatctt tgatagtggg tggaattggc
      241 attatgaata taatgcttgt atcggttacg gaaagaacac gcgaaatagg aattcgttta
      301 tctgttggtg cccgcacgtc agatattatg attcagtttt taactgaagc aattgtacta
      361 agtcttgccg gcggtttgat aggtattctt ttatcaatag gaataatatt tattttaaat
      421 aattatacca gtatggcagc agtaataaat ccgcagataa tttttatagc tgttagtttt
      481 gcaggggtaa ttggaatatt ctttggtttt tatcctgcaa agaaagctgc taatctaaac
      541 ccaattgacg cattaaggta tgagtaattt taagaaagag aattttgaaa tgaaaacatc
      601 atattatttt atttttataa ttttttgttt aagtgttttt aataattctt attctcaaaa
      661 attattaaca cttgatgatg cagttacaat tgcgcttaat caaaatacta atttagttaa
      721 gagtacaaat tcccttgcta cctttcaggc tggagtaaaa aatgcttatg gaaatctaat
      781 tcctacttta agtttaggtg gaaattttgg ctggcaaaga gtaactaata gcgaacgtac
      841 aactcagctt gattctcttg gtaaccagat aactaccggt ccaacacagt atgattccag
      901 gttctggtct gtctctgcgg gtggaagtgt tacactcttc aacggtcttt ctaatcttgc
      961 tcaaataagt aaaagtaaat ccgattttga atcagctaag ctaacccttg aaaaattaaa
     1021 acaagatatt gttctacagg caataacatt gtacacacaa ataataagtt atcaaaaact
     1081 attaaatttt caggaggaaa atttaaaata taatgaaggt ttgcttgcac aaataaaaga
     1141 aaaatttgat ttgaaaatgg ttccgataag tgatgtgtat tcacaagaag ctcaaaccgc
     1201 taattcaaaa gttttctatc ttcaggcaaa gaataatttg gaaatagcaa aaataaatct
     1261 gctcaattat ctctcgattg atgtttctca gaactatcaa tttgattcaa cggcagctaa
     1321 cattaaagat tcaactttaa tcaaaagtaa tattgatagt ctgtatcaat acgcattgaa
     1381 caacagaaaa gattatcaaa gcatgttatt tgagctggaa agttcacaac atcaattaac
     1441 aattgcacga agcgggcttt ttccaaatct ttcggccaat tatggatttg gttcaagttt
     1501 taatgatccg gctaatattt ttaatcaaaa agtttacagc cttggacttt ctttaaacat
     1561 tccaatcttt tcaaactgga gtacagaaaa cgcaattcaa tcagcagatg ttcaaattca
     1621 aaatagtaat gaagatctga aagttttaga actaagtatt aagagtcagg ttaaaactgc
     1681 aaatttaaat ttagaaacta caaaacagca gcttgatgct tctgaaactg ctttaacagc
     1741 ttcaagagaa agttggaaaa ttgaaactga gacatataat ctgggtgctg caacgtatct
     1801 ggatttacaa ctatcctaca acaattatct gcaagctcag tataataaaa ttagcaatga
     1861 gtttagttat ataatatctc aatacacttt gcttaatgca ttaggaaatt attaaatgag
     1921 atattttaat agtcatttat attaattgac ttaatgaata aactgataaa taaaatttca
     1981 aagatgaata gaacctctct gattggttca tcgattcttc tgctttgctt tattggattt
     2041 atcgattgga gtaccggtac agaattatct atttcaatat tttatctgat tccaattgca
     2101 atttgtgcat ggttcgttaa taagtggacc ggagtggtgt tagcaataat tagtataacg
     2161 atatggtttc tctccgatta ttttgactct tcatcatata atcatccaat tactccttat
     2221 tggaatgcat taattatgct tggaattttt gttttggttt cagagcttct ttctgctcta
     2281 aaaagagtga ttgacaggga aaacatttta gctctgaata ttcaaaaaaa tttgctgcca
     2341 gggaaaaatc ctgaaatttc agattataaa gtatttagta tctgggagcc cacaagagtt
     2401 gttagcggcg attattatga ttttatccaa ctgaaggaaa atgtattagg tatttcgctc
     2461 gctgatgttt gcggacacgg ctttccagca gctttattga tgtcaaacgt gcaagctaca
     2521 tttcgaatta ttgctggtca taatcatgca cctaaagaag tctgtgatca cctgaatagt
     2581 attctaatct cgtataatat gccggataaa tttaccagtt ttttctatgg gattttggat
     2641 gctgaaaata aagaattcac ttactcaaac gcaggtcatc cgcctccaat tatcttgcgg
     2701 gcaaatggta aaattgatcg tctttcagtt ggaggttttt tacttggagt aaatcctatt
     2761 gccaaatacg aacaggaaac ggtttatatc gaaagtggag atatcatatt gctttat
//
LOCUS       DASWBY010000328         4024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_49017, whole genome shotgun sequence.
ACCESSION   DASWBY010000328 DASWBY010000000
VERSION     DASWBY010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49017"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VF870_06145"
     CDS             <1..182
                     /locus_tag="VF870_06145"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX9251803.1"
                     /translation="VALGSDFDGIGTNVVVGLEDVSKYPALTLALLQHGYTEQEVAKF
                     LGGNFRRVFEQVCKN"
     gene            complement(248..1261)
                     /locus_tag="VF870_06150"
     CDS             complement(248..1261)
                     /locus_tag="VF870_06150"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9251804.1"
                     /translation="MKKLNLILYITLVTALFFAADAQTLKTGTTSAQILKINVGPRAI
                     GMGGAFTSIADDITSVYWNPSGTANIQSNEVYFNHSNLYLDISNDFAAISTNVSGFGS
                     IGAFVSVLSVDEMLVRTVEQPEGTGEYFDYGSIVVGLNYARFLTENFSIGFNAKYISE
                     SIWNMSATGFALDVGTLYKIPVLNELRIAASVSNFGTKMQLAGRDVTELVPAGAGGGN
                     YINSNLELGKFDLPLLFRFGISADVVKEGTSRLTVAVDAIHPNDHTEYINSGLEYAWN
                     EIIHIRAGYNSLFEKDSEKGLTFGFGLNYRIVDLIKVKLDYAYQDFGRLSEVHYFSVG
                     INF"
     gene            complement(1283..>4024)
                     /locus_tag="VF870_06155"
     CDS             complement(1283..>4024)
                     /locus_tag="VF870_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251805.1"
                     /translation="DNREFKYYPFENDTLRRGLGLQIDGRAFQWSNSLAANALFMVWT
                     ITNVSEKPLDKVFFGIYGDPDLGGQSDNDDDNGLFIKPYGDEVQNIPVYARSMVYFFD
                     NPQTPTGLNGLPVYYLGCKFLESPGNPNDGIDNDGDGLIDERQDDGIDNDGDWDPETD
                     DVGVDGIPLTLDQGEGDGVPTRGLRLSDGRPDPLAPGEPNFEYTDLDEDDQIGLTSFA
                     SSSWNTDLKVGDDEVIWNRNVPGSFSEIIQDADIVFTFGSGYISLAPGESKRISMAFL
                     FGENLSDLLTTAETVQDIYNKNYNFFRPPSLPTLSAVPGDKKVTLYWDTKSESSVDPI
                     TGEDFEGYVIYRSTRPDFSDVQTITDGRGSSFLYDPLKDTQGFEAKWDLTNGWKGYHP
                     VPYQGRGVHYYLGDDAGLRHSFVDSNNVINGQTYYYAVVAYDHGDSLGIPPTETTKKI
                     SQDPITSNLIFDLNTASVIPGPRASGYSQPSISITDVQHVQGLANGNVDFSILDDLDV
                     VNDKYKLTFKDTLFVDEEKILAKNYSVLGEKINTESFYLFGYKNTNLQNSYIIDDDNL
                     VVKDLNGVVYQKNVDYIINYEKGTIKRDSNTTMPDNSEYTISYRNYSIYQSQYLNGED
                     GNPVFNGIKLKVSDYNSLAIDLERTKWSDSNVQIPYNITLTPLGNRKKQYPGDYIITF
                     YNENSYPAKKRVPGKPLQDILVNYKVEEVSTGISIPVMTLLEERAKNDTTLSLGDEII
                     LFLPGTAGLSTDTLSWGITFTKTASGDSIVPGAGNVLLIYTTRPFTKSDVFTLNTSSG
                     FVSNEDAKSRLDNIYVVPNPYVGTNDIEPTDKLPNQNRGERRIYFENLPMKCTIRIFT
                     LSGELVTTLEHESGVDNGREFWNLLNRDGFSVSYGLYIAHIDAPEVGEKLIKFALIK"
BASE COUNT         1233 a          826 c          673 g         1292 t
ORIGIN      
        1 atgttgcttt aggttctgat tttgatggaa taggtaccaa cgtagttgta gggttagagg
       61 atgtttcaaa atatccggct ttaactttag ctttattgca gcatggttac accgagcagg
      121 aagtagcaaa atttttaggc ggaaatttta gaagggtttt tgagcaagtt tgtaaaaatt
      181 aatttttggt aaaaaaagag cggatttata aaatccgctc actaaaatta attcaaggta
      241 ttaaagttta gaaatttatt cctacagaaa agtaatgcac ttcagacaaa cgtccgaaat
      301 cctgataagc ataatcaagt tttactttta ttagatctac aattctgtaa ttcaatccga
      361 atccaaaagt caaacctttt tcagaatcct tctcgaataa cgaattatac cctgcccgaa
      421 tatggattat ttcattccag gcatattcta aacctgaatt gatatactcc gtatgatcat
      481 ttggatgaat tgcatcaact gcaactgtta agcgagaggt tccctctttt actacatcag
      541 cagatattcc aaatctgaat agtaatggta aatcaaattt accaagttca agattggaat
      601 taatgtagtt accaccaccg gcaccagctg gaacaagctc agtaacatct cttcctgcca
      661 attgcatttt tgttccaaag ttcgatacgc ttgcggctat tcttaattcg tttaagactg
      721 gaattttata cagtgtacca acatctaaag caaatcctgt tgcgctcata ttccagatag
      781 attcgctgat gtacttggca ttaaacccga ttgaaaaatt ctcagtcagg aaacgtgcgt
      841 aatttaatcc aaccactatt gatccgtagt caaaatattc tccggtaccc tccggttgtt
      901 caactgtacg aacaagcatt tcatcaacgg aaagtactga aacaaaagca cctatcgagc
      961 caaatccact aacattcgtt gaaattgcgg cgaagtcatt gcttatatcc aggtataaat
     1021 ttgaatgatt aaagtatact tcatttgatt gaatattagc agtaccactg ggattccaat
     1081 aaactgaggt aatatcatct gcgattgaag taaatgcacc acccatgccg attgcacgcg
     1141 ggccaacatt tattttcaat atttgcgctg aagttgtccc ggtcttaagt gtttgagcat
     1201 cagcagcaaa aaacaatgca gttactagag ttatatataa gattaagttt aattttttca
     1261 tgatttttct cctgcagctc ttttacttaa ttaaagcaaa cttgatcaat ttttcaccca
     1321 cctcgggtgc atcaatgtgg gcgatgtata acccgtaaga aacgctgaat ccatccctat
     1381 tcaacaagtt ccaaaattct ctgccattat caactccaga ttcgtgctcc aatgttgtaa
     1441 ccagctcacc acttagcgta aatattctta ttgtgcactt catgggtaga ttttcaaaat
     1501 agattcttct ttcgccacga ttttgatttg gcagtttatc agtcggttca atatcatttg
     1561 ttccaacata aggattaggc acaacgtaaa tattgtctaa tcttgattta gcatcctcgt
     1621 tactgacaaa accagatgaa gtattaagtg tgaacacatc actctttgtg aatggccttg
     1681 ttgtataaat taataaaaca tttccggcac cgggaacaat cgaatctccg ctggccgtct
     1741 ttgtaaaagt tataccccag gacaaagtat ctgtagaaag tccggctgtt cccggtaaaa
     1801 ataaaattat ttcatcacct aatgataaag tagtatcatt ctttgctctt tcctccagca
     1861 gggtcataac gggtattgag attccggtcg atacttcttc aaccttgtag ttaacaagaa
     1921 tatcctgcag gggtttaccg ggcactcgtt ttttcgccgg ataagaattc tcattataaa
     1981 atgtaatgat ataatcgcca ggatactgtt tctttctgtt accaagtggt gtcaatgtaa
     2041 tattatatgg tatttgcaca ttagaatcag accactttgt tctttccaga tcaatcgcaa
     2101 gtgaattata atcagaaact tttaatttga ttccattaaa aaccggattt ccatcttccc
     2161 cattaagata ctggctctgg tagatcgaat aatttctgta gctaatagta tactcactgt
     2221 tatcgggcat cgtggtatta ctatcccgct tgatagtacc cttttcataa ttaattatgt
     2281 agtccacatt tttttgatag accacaccat tcaaatcttt aactactaaa ttatcgtcat
     2341 caattatata actattttgc agattagtat tcttataacc aaacaggtaa aatgattctg
     2401 tattgatttt ttctccaagc acgctataat tttttgccag tattttttct tcatcaacaa
     2461 agagtgtgtc cttaaaagtc agtttatatt tatcattaac aacgtcaaga tcatcaagta
     2521 tagaaaagtc aacatttcca tttgcaagac cttgaacatg ttgaacatcg gtgatactaa
     2581 ttgaaggctg agaataaccg cttgctcttg ggccaggaat tactgaggct gtgttcagat
     2641 caaaaattaa atttgatgta attggatcct gagaaatctt tttagttgtt tccgttggcg
     2701 gaatgccaag cgaatctccg tgatcatatg cgacaacagc ataatagtag gtttgcccat
     2761 ttatcacgtt attagaatca acaaatgaat gtcttagacc agcatcgtca cctaaataat
     2821 aatgaacacc cctgccctga taaggaacag gatggtaacc tttccagcca ttcgttaagt
     2881 cccacttcgc ttcaaaaccc tgtgtatctt ttaatggatc atatagaaaa ctcgatcctc
     2941 ttccatctgt aatagtttga acatcggaga aatcaggacg ggtgcttctg taaattacat
     3001 atccttcaaa gtcttcaccg gttattggat caacgctgct ctctgattta gtatcccaat
     3061 aaagagtaac ttttttatct ccgggaaccg cacttaaagt aggcagtgat gggggacgga
     3121 aaaagttata atttttattg taaatatcct gtactgtttc tgcagtggta agtagatcac
     3181 ttaagttttc accgaacagg aatgccatag atattctctt tgattcccct ggcgccaatg
     3241 aaatgtagcc tgaaccgaat gtgaaaacaa tatctgcatc ctgaattatt tcgctaaagc
     3301 tgccgggaac atttctattc cagataactt catcatcacc aacttttaaa tctgtattcc
     3361 aggatgagct tgcaaaacta gtaagcccaa tttgatcgtc ttcatcaagg tcggtgtact
     3421 caaaatttgg ttcgcccggt gctaaaggat caggtcttcc atcagatagc cttaagcctc
     3481 ttgtgggaac accatcacct tctccctgat caagagtcaa aggaattcca tcaaccccaa
     3541 catcatctgt ctcaggatcc cagtctccat cattatctat tccatcatcc tgtctttcat
     3601 caattaatcc atcaccatca ttatcaatcc catcgtttgg atttccgggg ctttcaagaa
     3661 atttacatcc taaataataa acgggcagtc catttaaacc agttggagtt tgagggttat
     3721 caaaaaagta aaccatgctt cttgcataaa ccggaatatt ttgaacctca tcgccgtatg
     3781 gttttataaa taaaccatta tcatcatcgt tatcactttg gccacctaag tcaggatcac
     3841 cgtatatccc gaagaatacc ttatccagag gtttttcact aacatttgtt atagtccaga
     3901 ccataaacaa tgcatttgct gcaagtgaat tactccattg gaaggcacga ccatcaattt
     3961 gaagtcctaa tcctcttcgt aaagtatcat tttcaaaagg ataatatttg aactccctgt
     4021 tatc
//
LOCUS       DASWBY010000329         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_14720, whole genome shotgun sequence.
ACCESSION   DASWBY010000329 DASWBY010000000
VERSION     DASWBY010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14720"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VF870_06160"
     CDS             <1..1088
                     /locus_tag="VF870_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX9251806.1"
                     /translation="REMPLKYLDLVALAGAADIVPLVDENRLLVKEGLDSININPRPG
                     ILALIESSSLQPGNLNSGQIVFTIAPRINAVGRLGDAKRAVELLITKSKDEALTLAKV
                     LETENYERRKIDVDTFDNALQLVESSIDLDNDLAIVLHQEEWHPGVIGIVASRLVEKY
                     YRPTIMLTTIDGIAKGSARSITNFNIYEALEKCQDMLIHFGGHQAAAGLAVELDKFQE
                     FKDKFNQIVKETITGDDLFPEIIIDSKIKLSEITPKFLRILDQFAPFGPQNMRPVFLS
                     EGVEIFNNPRLVGNKHLVACFKQNGNDKVFDTIGFNMREYLDLLSDKKLKVDIVYTID
                     KTVREGRTYPQLRLKDLRINKTETQEN"
     gene            1091..>1719
                     /locus_tag="VF870_06165"
     CDS             1091..>1719
                     /locus_tag="VF870_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9251807.1"
                     /translation="MSGHSKWATIKRKKAVLDSKRGKIFTKLIKEITIAARDGGDPNG
                     NPRLRLAVDNAKAANMPLDNIERAIKKATGELEGVTYHELTYEGYGPAGVAVLVEVAT
                     DNKNRTVAEVRHLFSKNGGSMGETGSVAWMFDRKGVITLPAEGKKEDDIMEIVLEAGA
                     DDLTTEEDFFEVTTSIESFETVRRTLVDKNFALENASLQWIAKNMIEVKG"
BASE COUNT          598 a          257 c          369 g          495 t
ORIGIN      
        1 gacgtgagat gcctttaaag tatcttgatt tggttgcgct tgcaggcgca gcagatattg
       61 tccctcttgt ggatgaaaac agattattag ttaaagaagg tttggatagt ataaatatta
      121 atccaagacc gggaattctt gctttgatag aaagcagtag tcttcagcca ggtaatctta
      181 attccggtca gattgttttt actatcgcac caagaattaa tgcagttggc agattgggtg
      241 atgctaaacg tgctgttgaa ttattaataa ccaaaagcaa agatgaggct ctgacacttg
      301 caaaggtttt ggaaacagaa aattatgaac gaagaaaaat agatgttgat acttttgata
      361 atgcattaca gcttgtagaa agctcaattg atcttgataa tgatcttgct attgtgcttc
      421 accaggagga atggcatccg ggagtaattg gtattgttgc atcacgcctg gttgaaaaat
      481 actatagacc tacaattatg ttaaccacaa tcgatggaat tgcaaaggga tcggcgagaa
      541 gcattactaa ttttaatatt tacgaagcac ttgaaaaatg tcaggatatg ttaatccatt
      601 ttggcggaca tcaggctgcg gctggtttag cagtggaact ggataagttt caggagttta
      661 aagacaagtt caatcaaata gtaaaagaaa caattaccgg cgatgatctt tttccggaaa
      721 ttattattga ttctaaaatc aaactttcag aaataactcc aaaattttta agaatacttg
      781 atcaatttgc tccctttggt ccacaaaata tgcgtccggt atttctttct gagggagttg
      841 aaatttttaa caatccaaga cttgttggaa acaagcatct tgttgcatgt tttaagcaaa
      901 acggaaacga taaggttttt gatactattg gctttaatat gcgtgaatat ttagatttgt
      961 tgtcagataa aaaattaaaa gtggatatcg tttataccat tgataaaacg gtaagagaag
     1021 gcagaacata tccgcaactt agactaaaag atttaagaat aaacaaaaca gaaactcagg
     1081 agaattagta atgtccggtc actcaaagtg ggcaacgata aaaagaaaaa aagccgttct
     1141 tgattctaaa agaggtaaaa tttttaccaa acttataaaa gaaattacaa tcgcagccag
     1201 agacggcggt gatcccaatg gaaacccaag attaagatta gcggttgata atgctaaagc
     1261 cgcaaacatg cctttagata atattgaacg tgctattaaa aaagctaccg gcgaactgga
     1321 aggtgtaact tatcacgaat tgacttatga gggctacggt cctgcaggag ttgcagtact
     1381 tgttgaagtt gctactgata acaaaaacag aacggttgct gaagttagac atctctttag
     1441 caagaatggc ggcagtatgg gtgaaaccgg ctcagttgca tggatgttcg atagaaaagg
     1501 tgtaataact cttcctgctg aaggtaaaaa agaggatgac ataatggaaa tagttttaga
     1561 agcaggagca gatgatttaa caactgaaga agattttttt gaagttacaa cttcgattga
     1621 atcttttgaa actgtaagaa ggaccttggt tgataaaaac tttgctcttg aaaatgcttc
     1681 actccagtgg attgcaaaaa atatgattga agtaaaagg
//
LOCUS       DASWBY010000330         8016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_726128, whole genome shotgun sequence.
ACCESSION   DASWBY010000330 DASWBY010000000
VERSION     DASWBY010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726128"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VF870_06170"
     CDS             complement(<1..532)
                     /locus_tag="VF870_06170"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX9251808.1"
                     /translation="MIELQRTISKPATLSGIGLHTGTECTMTFKPAPINSGIKFIRVD
                     LGGNPEIPAIADNVVDVSRGTTIGIGEAKVYTVEHVLAAIAGLQIDNINVELNGIEPP
                     IGDGSSIAYVNALLEAGIEKQDAPKDYLIIDETVTYSDESRQVDIVALPLDKYRLTVM
                     VDYQNPALGSQHTGLFD"
     gene            complement(635..1678)
                     /gene="lpxD"
                     /locus_tag="VF870_06175"
     CDS             complement(635..1678)
                     /gene="lpxD"
                     /locus_tag="VF870_06175"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX9251809.1"
                     /translation="MKISILEIASLINGSITGNNNLEINNVSKIEEAKPGDLTFLYLS
                     SYDKYFSTTQASAIIVKTGFPKSRNDITYIEVNSPEKAFFTILKKYFTPAFPLSGIDP
                     SAFIHPTARLSDNAAIGKNVVLSSGSKIGDSTKIFHNTVISENVSIGNNCLIYPNVTI
                     RENCIIGNNVIIHSGTVIGSDGFGYTPNEKGEYEKIPHIGNVVIEDDVELGSNVSIDR
                     AAMGSTIIKRGCKIDNLVQIAHNVILGEDTVISAQTGISGSTKIGKHCILAGQAGLVG
                     HIELGDNVVITAQSGVSKSILKPGYYMGYPAREMKTTQKINAHIRNLPEYAKKLSELE
                     SEIKKLKEDLKAT"
     gene            complement(1689..2201)
                     /locus_tag="VF870_06180"
     CDS             complement(1689..2201)
                     /locus_tag="VF870_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX9251810.1"
                     /translation="MKKLLAISFLLITSSISFAQLKIGYIDSDAIMDNLPDVQDARQK
                     LDASIQEWQTELNKLEADWKAKYDDYEKRKLIMTDQTRAETEAALVQLETQIAQYREK
                     KFGTNGELFQKQDELMKPVQNKIFGALKELAEAENYDFVFDRSGDIMILFAKEKYDLT
                     SQVLEKLKLK"
     gene            complement(2307..2831)
                     /locus_tag="VF870_06185"
     CDS             complement(2307..2831)
                     /locus_tag="VF870_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX9251811.1"
                     /translation="MKSYILVLVFLVLGFTSFAQTQLKIGYVDSEVILTQFSESIKAQ
                     GDLDALTSKLSAQLDSMTLAYQQSIADYQKQAETMNDLKKTEFQQKIVKMEQDIMEFR
                     RAKFTQGTGEIYKKQEELFAPIKAKIYAAIEKVSKEENMQFVFDKSGDIILLYADSSF
                     DITFKVLDRLKRGS"
     gene            complement(2867..5368)
                     /gene="bamA"
                     /locus_tag="VF870_06190"
     CDS             complement(2867..5368)
                     /gene="bamA"
                     /locus_tag="VF870_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX9251812.1"
                     /translation="MFISSPMRLLKYFLVFFILVFSTAIFSQQRTTYKVLGITVDGNK
                     SSDANTIIVSTGLKVGDEIQVPGDKTINAIKNLWALNIFSDVQILIDKELSDGVFLLI
                     KVQEYPRLEKVVIEGNDEIDSDDIEKKITFLRGSILKPQDVAKIIQKINGLYEEEGYY
                     NTEITAQYYRYFSADTLDGDVLVRWQNINDLADEYEVEYNESEAKSSNLINKIKDRLL
                     LKYLIKEGNEVTVREIAFNNNTAFDDDDLKSEMDDTSEKVWWKFWSGAKFDPKKFKKD
                     KELIVNFYKKNGYRDAEIISDSLIYSNDKKDVKIVMDLFEGPQYKIRNIKWEGNTVYP
                     SYALNERLDFAPGDVYDLEKFEQNLRGNEKQSDVSALYLDNGYLTFNLKTTETKVAED
                     SVDITIRVEERNQFRVGRVDIYGNDKTKEKVIRRELYAIPGDFFNRGLLFRSVQNLAN
                     LQYFNVEQLYGPEGITTKLSSDSTVDIGFKVSEKSSDYLNASVGYSGSFGFSGAIGVT
                     LTNFSIAEPFRLGAGQVLSFNWQFGVGSLYRTFTLGFTEPWMFDTPTSVGVEVFDTRQ
                     QYVYDLRQSGATLRVGRRLKWPDDFFYVQGRFRYQYNNVIEGQNYYKEGVFNQFSVGA
                     LISRKNIDNPIFPSNGTSLQLDVELSGGRFLPGDVDYLKLNFLTEWYRRLFNSNRVAL
                     YTMANVGYLDEIVPGTNIQPFEFFYMGGNGLVIATTALRGYDDRSVGPKDPRTGYVIG
                     GRVMAKFGAEFRVAVTLEPIPLYLLMFAEAGNVFENFQKTDIFDLRRSVGFGARLLIN
                     PIGLIGFDLGYGFDRKMTDGKDPEWLFHFQFGKGF"
     gene            complement(5337..6164)
                     /locus_tag="VF870_06195"
     CDS             complement(5337..6164)
                     /locus_tag="VF870_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX9251813.1"
                     /translation="MNKKSSASDEQVQEQLKKSGEIPKHIAIIMDGNGRWAKKRGLPR
                     VAGHKRGVDTVKEIVEACAEIGVKFLTLYTFSTENWKRPKDEVSTLMRLLLNSLRDRV
                     NELNENDIRLTTIGNIDALPAAVRKQLEFDIERTKNNKKMVLNLALSYSGRWELLEAI
                     KQITDSAIQGKIKSEDIDENIVSSFLTTKNIPDPDLVIRTSGEFRVSNFLLWQIAYSE
                     FIITETLWPDFSKFDLYDAVKIFQKRERRFGKVSEQITKKEKEKGPENVHFLPNEIA"
     gene            complement(6319..6750)
                     /locus_tag="VF870_06200"
     CDS             complement(6319..6750)
                     /locus_tag="VF870_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251814.1"
                     /translation="MNTERRRDVDLLIEEFWKKGYLTLFRKFGTYLPEPSNIGGFEVD
                     VIARQKDNFAIGITLSDQDFKDPSFIKNKLSYLAQRQTRGSNKKVQLFVGVSLLNLKY
                     AKALLDELDPETKKNIKLFQITEKPDLSIRKESKSGKILFS"
     gene            complement(6763..7011)
                     /locus_tag="VF870_06205"
     CDS             complement(6763..7011)
                     /locus_tag="VF870_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251815.1"
                     /translation="MKTEIFETAIKNRNRLRFLYGLNEVLIEPYYITMEKSGSKVIYG
                     KVFQSSEIKKFNYKKIANIKVLEKKRFSPIIPIIPLAS"
     gene            complement(7024..7674)
                     /gene="lexA"
                     /locus_tag="VF870_06210"
     CDS             complement(7024..7674)
                     /gene="lexA"
                     /locus_tag="VF870_06210"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX9251816.1"
                     /translation="MEKKQLTDRQEDILNFIQQFLQENGYPPTLREIGKRFDISSTFG
                     VKRHLEALTKKGYLNILSNASRGITITRDEFESTAAINITEINNNSKIPIVGRVAAGS
                     PITAEENIEGSIVIDPGFLKKDVDSFALKVKGDSMIEAGIFEGDLVIISPKTNAISGD
                     IIVARIDDEVTVKIYENKNQQIRLIPQNKLYEPIVIKNKNEFSIVGKVTGVVRWLN"
     gene            complement(7787..>8016)
                     /locus_tag="VF870_06215"
     CDS             complement(7787..>8016)
                     /locus_tag="VF870_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251817.1"
                     /translation="TLVLITSDHETGGMAITKGNRDASNLELSYTTKDHTASPVGIFA
                     FGPGEERFRGIMNINEIGKKLFYLLDPAYKF"
BASE COUNT         2474 a         1510 c         1156 g         2876 t
ORIGIN      
        1 tatcaaacaa acctgtgtgc tggcttccga gtgcaggatt ttgataatca accataacag
       61 taagtctgta tttatccaaa ggaagggcta cgatatcaac ctgcctggat tcatctgaat
      121 aagttactgt ttcatcgatt attaaataat cttttggagc atcttgtttt tctataccgg
      181 cttctaataa agcatttaca taagcaatag aactaccatc accaatcgga ggttcaatac
      241 catttaactc aacattaata ttatctattt gtaaaccggc aatagccgca agtacgtgtt
      301 caactgtgta aactttggct tcaccaattc caattgttgt ccctcgtgaa acatctacaa
      361 cattgtcggc tatcgcagga atttcaggat taccgccaag atcaactctg ataaatttaa
      421 tacctgaatt tattggtgca ggtttgaaag tcattgtaca ttccgtgccc gtatgcagtc
      481 caattccgct taaggttgcc ggtttagata tggttctttg aagttctatc attattgttc
      541 cattttttta taaaattgat gaaaaaatag taagatgatg gactaaatac aattatatgg
      601 atgtgattga aaaagataag aatgaagaaa atattcaggt agcttttaaa tcttctttta
      661 attttttgat ttcactttcc agttctgata atttttttgc atactcaggc aaattgcgga
      721 tatgagcatt tattttctga gttgttttca tctcgcgtgc aggatatccc atataatatc
      781 ccggctttaa aattgatttt gagacccctg actgagcagt tataacaaca ttatcaccaa
      841 gttctatatg accaactaaa cctgcttgac cggcaagaat acaatgcttt ccgattttag
      901 tacttccgga aattcctgtt tgtgcagaaa taacagtgtc ttcaccaagt attacattat
      961 gtgcaatctg aacaagatta tctattttac accctcgttt gattatggtt gaacccatag
     1021 cagcacgatc aatagaaaca tttgaaccaa gttcaacatc atcctcaata actacattac
     1081 caatgtgtgg aattttttcg tactcacctt tttcatttgg ggtgtatcca aatccatcac
     1141 ttcctattac agttcccgaa tgaataataa cattattgcc aatgatacaa ttttctctga
     1201 tagtaacatt aggatagatg aggcagttat ttccaattga aacattttcc gaaataacag
     1261 tgttgtgaaa aatttttgtg ctatctccta ttttagatcc ggatgaaaga acaacatttt
     1321 ttccgattgc agcattatct gaaagtcttg ctgttggatg aataaatgcc gatggatcaa
     1381 taccgctaag agggaaagca ggagtaaaat attttttgag aattgtgaaa aaagcttttt
     1441 caggcgagtt aacttctatg tatgtaatat catttctgga tttaggaaat ccagtcttta
     1501 caattattgc agaagcctga gtggttgaaa aatatttatc gtaagaagag agatacaaga
     1561 aagttaaatc accgggttta gcttcttcaa ttttagaaac attattaatt tcaagattat
     1621 tattcccggt tatactgcca ttaattagag aagctatttc caggatacta atcttcattt
     1681 tgtattactt attttagttt aagtttttcc agaacctgtg aagtcaaatc atatttttct
     1741 ttagcaaaaa gaatcatgat gtcgccactt ctatcaaaaa caaaatcata attttctgct
     1801 tcggcgagtt cctttaatgc accaaaaatt ttattctgaa caggtttcat aagctcatcc
     1861 tgcttttgaa acaattcgcc attagtgcca aatttctttt cgcgatactg ggcaatttgg
     1921 gtttctaact gaacaagtgc tgcttcggtt tcggcccttg tctgatcagt cataattaat
     1981 tttcttttct cataatcatc gtatttagct ttccagtctg cttcaagctt attaagttct
     2041 gtttgccatt cctgaataga tgcatcaagt ttttgccttg catcctgtac gtctggaaga
     2101 ttatccataa tcgcatcaga atcgatatag ccgattttaa gctgcgcaaa agaaattgaa
     2161 gatgtgatta atagaaaaga tatagctagt aactttttca tcggaaaatc ctattgtttg
     2221 attttattta atcaaaatat cataaataaa aaaataaagt tctccccaaa aaaatatggg
     2281 gagaactaag aaaacttaaa cactaattag ctgcctcttt ttagtcgatc caaaacttta
     2341 aaagtaatat caaaagatga atcagcataa agaagaatta tatcgccact tttatcaaag
     2401 acaaattgca tattttcttc tttagaaact ttttctattg cagcatagat ctttgcttta
     2461 atcggagcaa aaagctcttc ctgtttttta tatatttcgc cggtaccctg agtaaatttt
     2521 gctcttctga attccataat atcctgttcc atctttacaa ttttctgttg gaattctgtt
     2581 ttctttaaat cattcattgt ttcagcttgc ttctggtaat ctgcaattga ttgctgatat
     2641 gctaaagtca ttgaatccaa ttgagctgat aatttacttg tcaaagcatc aaggtctcct
     2701 tgagctttaa ttgattcgga gaattgggtt agtataactt cagaatcaac ataaccaatt
     2761 tttaattgtg tctgtgcaaa tgaagtaaaa cctaaaacta aaaacacaag cactaaaatg
     2821 taacttttca ctattttcct cgttctttaa ttattgtaca aattatttaa aaacctttac
     2881 caaactgaaa gtggaacagc cattctggat ccttaccatc agtcattttt ctatcaaaac
     2941 cataaccaag atcaaatcct atcaaaccaa taggatttat taataacctt gcgccaaatc
     3001 caaccgatct gcgaagatca aaaatatctg ttttttgaaa attttcaaag acattacctg
     3061 cttctgcaaa cattaataaa taaagaggta ttggctctaa tgttacagca actctaaatt
     3121 ctgcaccaaa ctttgccatt actctgccgc caataacgta acctgtcctt ggatctttag
     3181 gtccaacaga tctgtcatcg taacctctaa gtgctgttgt agctattact aatccatttc
     3241 cgcccatata gaaaaactcg aatggctgaa tattagtacc gggaacaatt tcatctaaat
     3301 atccaacatt agccattgta tatagggcta ctctattaga gttaaataat cttctatacc
     3361 attccgttag aaaatttaat tttaaataat caacatcacc gggtaaaaat ctaccgccgg
     3421 aaagttcgac atctaattgt aaagaagtac cgtttgaagg gaatatagga ttgtctatat
     3481 tttttctgga tataagggct cctacagaaa attgattgaa tactccttct ttataatagt
     3541 tctgaccttc aataacattg ttatattgat atctaaatct gccctgaaca taaaagaaat
     3601 cgtccggcca ttttaatctg cgtccaactc taagagttgc tccagactgc cgtaaatcat
     3661 aaacatattg ctggcgggta tcaaacacct ctactcctac tgaggttgga gtatcaaaca
     3721 tccaaggctc tgtaaaaccc aaagtaaaag ttctataaag actaccaaca ccaaactgcc
     3781 aattaaaact taatacctgc ccggcaccaa gtctgaaagg ttctgctatt gaaaagtttg
     3841 tcagcgtaac tccaattgca ccgctaaatc caaaacttcc gctatatcca acagaagcat
     3901 taagataatc gcttgatttt tccgatactt tgaacccaat atctacagtg ctgtcgctgg
     3961 aaagttttgt ggttattcct tcaggaccat ataactgttc gacattaaag tactgaaggt
     4021 ttgcaagatt ttgtacgctg cggaataata agccgcggtt aaaaaaatca cccggaatag
     4081 catataattc tcttcgaata accttttctt tagtcttatc atttccataa atatcaactc
     4141 tacctactct aaactgattt ctctcttcta ctctaattgt aatatcaacc gagtcttctg
     4201 caacttttgt ttcagttgtt ttaagattaa aagtaagata accattatct aagtaaagcg
     4261 cagaaacatc tgattgtttt tcattgccgc gcaaattttg ttcaaacttt tcaaggtcat
     4321 aaacatcacc aggagcaaaa tctaatcttt catttaatgc atatgaggga taaacagtat
     4381 tgccttccca tttaatattt ctgattttgt actgaggccc ctcaaacaaa tccattacaa
     4441 ttttaacatc ttttttatca ttggaataga ttaaagaatc agaaataatt tcagcatccc
     4501 tataaccatt cttcttgtag aaatttacaa ttaactcttt atcttttttg aatttcttgg
     4561 gatcaaattt tgcaccgctc caaaatttcc accaaacttt ttcagaagta tcatccatct
     4621 cgctttttaa atcatcatca tcaaaagcag tattattatt aaaagctatt tctcttacag
     4681 ttacttcatt accttctttt attaaatatt ttaatagcaa tctatctttt attttattaa
     4741 taagattaga tgacttcgct tctgattcat tgtactcaac ttcatattca tcggcaagat
     4801 catttatatt ttgccatctt acaagcacat caccatcaag agtatctgca ctaaagtatc
     4861 tataatattg ggctgttatt tctgtgttgt aatagccttc ttcttcataa agaccgttta
     4921 ttttctgaat aatttttgca acgtcttgtg gttttaatat tgatcctcta agaaaagtaa
     4981 ttttcttttc gatatcatca gaatctattt catcattgcc ttctataact actttttcaa
     5041 gtcttggata ttcttgaact tttattaata gaaatacacc atcacttaat tctttatcaa
     5101 taagtatctg tacatcagag aaaatattta atgcccaaag attttttatt gcatttatag
     5161 ttttgtctcc gggaacttga atttcatcac cgacttttaa tccggttgaa actataatgg
     5221 tattcgcatc agatgattta ttcccatcaa cagtaattcc gagtacttta tatgttgttc
     5281 tctgctggga aaagattgca gtagaaaaaa ccaaaataaa aaacactaaa aaatatttaa
     5341 gcaatctcat tggggaggaa atgaacattc tccggacctt tttccttttc tttcttcgtt
     5401 atttgctcac ttactttacc gaatcttctt tctctttttt gaaatatttt aacagcgtca
     5461 tataaatcaa atttagaaaa atccggccaa agagtttctg taattataaa ctcagaataa
     5521 gcaatttgcc agagcaaaaa attacttacc ctaaattctc cacttgttct tattaccaaa
     5581 tcaggatcag gaatattttt tgtggtaaga aatgatgata caatattttc atcaatatct
     5641 tcacttttaa tttttccctg aattgcggaa tctgtaatct gttttattgc ttcaagtaat
     5701 tcccatcttc cgctgtaact taaagccaga tttaacacca tctttttatt gttttttgtt
     5761 ctttcaatat caaattccaa ttgctttcta acagcagctg gtaaagcatc aatatttcca
     5821 atggtagtca gacgtatatc attctcatta agttcattta cacgatcacg taaactattt
     5881 aacagaagcc tcattagagt tgaaacttca tctttcggtc gcttccagtt ttctgttgaa
     5941 aaagtgtaaa gggtaagaaa cttaactcca atttcagcac aggcttcaac tatttcttta
     6001 acagtatcaa cacctcgttt atgaccggca actcttggca aacctctttt ttttgcccaa
     6061 cgtccgtttc catccattat gatggcaatg tgtttaggaa tttcacccga tttttttaat
     6121 tgttcctgta cctgctcatc ggatgcagag ctttttttat tcaaaatcca ttcctgttat
     6181 tgatttagcg tgtaaagtta attttaccgt gcacgaatgt caaggtaact aaaccttgct
     6241 aatttagttt aattgttgta aagcagagta tattaggatg gacttaggct caggattcct
     6301 gagcccaagc aaagtatttt atgaaaatag aatttttcct gacttacttt ctttgcggat
     6361 actaagatca ggtttttctg taatctggaa tagtttaata tttttcttcg tctccggatc
     6421 aagttcatct aaaagtgctt ttgcatattt tagatttaat aaagatacac ccacaaataa
     6481 ttgtactttt ttgtttgatc ctcttgtttg tctttgtgca agatatgata atttgttttt
     6541 tatgaacgaa ggatctttaa aatcctgatc gcttaaagtt attccaatcg caaaattatc
     6601 tttctgcctt gcaattacat ctacttcaaa gccgccaata tttgaaggct ccggcaagta
     6661 ggtgccaaac tttctgaata atgttaaata tccttttttc cagaattctt ctatcaataa
     6721 atcaacatct cttcttcttt ctgtattcat gatttcacct ctttatgatg caagcggaat
     6781 aattgggatt atcggagaaa atcttttttt ctccagcacc tttatgtttg caattttttt
     6841 gtaattgaat tttttaattt cacttgactg aaaaactttt ccgtaaataa ccttggagcc
     6901 gcttttttcc atagttatat aatatggctc tatcaatact tcattaagcc cgtataaaaa
     6961 tcttaaacga tttctatttt taattgccgt ttcaaatatt tctgttttca tatcatgctc
     7021 cttttaattt aaccatctta caacaccagt tactttgccc acaattgaaa attcattttt
     7081 gtttttaata acaattggtt catataattt attctgtgga attaatctta tctgctgatt
     7141 tttattctca taaatcttta cagtaacctc atcatcaata cgagcaacaa ttatatcacc
     7201 gctaattgca tttgtctttg gtgatattat cacaagatcg ccctcaaaaa ttccagcttc
     7261 aatcatgctg tcgcccttaa ctttaagtgc aaatgaatca acatcctttt tcagaaatcc
     7321 tggatctata acaattgacc cttcaatatt ttcttccgct gttatgggtg agccggcagc
     7381 aactctacca actatcggaa ttttgctgtt attatttatt tcagtaatgt taattgctgc
     7441 agtggattca aattcatcgc gcgtgatggt gatgccacga cttgcattac ttagaatgtt
     7501 caaataacct tttttggtga gagcttctaa atgtctttta acaccaaaag ttgatgaaat
     7561 atcaaatcgt tttccaattt ctctaagagt tggcggataa ccattctctt gcaagaattg
     7621 ttgaataaag tttaatatat cttcctgccg atctgttaat tgtttttttt ccattttgat
     7681 cacttttaat aattattaac tagtgctcat ttgttcactt caaatatagt gtacaagtgt
     7741 tcacgtgtca agagcagata taaaaaaatt ttaacttttt ttagatttag aatttatagg
     7801 cgggatcgag caggtagaat agtttttttc caatctcatt aatgttcata atccctctga
     7861 atcgttcctc ccctgggccg aacgcaaaga ttccgactgg agaagctgta tgatctttag
     7921 tagtataaga caattctaaa ttgcttgcat cgcggttacc ttttgtaatt gccattcctc
     7981 cagtttcatg atcagaggtg attaaaacaa gcgtat
//
LOCUS       DASWBY010000331         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_425551, whole genome shotgun sequence.
ACCESSION   DASWBY010000331 DASWBY010000000
VERSION     DASWBY010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425551"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VF870_06220"
     CDS             complement(<1..558)
                     /locus_tag="VF870_06220"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX9251818.1"
                     /translation="MKTALITGITGQDGAYLSKYLLEKKYKVIGITRNLQSGNSNGLN
                     FLGVENDVQLVEANVFDLSNIIRLLEKFKPDEFYNLAAQSSVGLSFEQPIGTLEYNII
                     SVANILEAIRIVDKKIKIYQSSSSEMYGNVAQSKLPIDEDFIIHPASPYAISKAAAHW
                     TCVNYREAYNLFISSGILFNHESVLR"
     gene            1145..1393
                     /locus_tag="VF870_06225"
     CDS             1145..1393
                     /locus_tag="VF870_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251819.1"
                     /translation="MREIRAKIDNPLSELISDDIFDLLDSHGLIDEKAVRDYQIRKKF
                     KQLRSGKLSAGDAIDAIRAEYPYLQFDTIRKIVYQISK"
     gene            1542..1826
                     /locus_tag="VF870_06230"
     CDS             1542..1826
                     /locus_tag="VF870_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251820.1"
                     /translation="MAVKTKKKNVKTSVKALPSPFNIYWARTNYLLFGLGMLLIIFGF
                     YFMSLGEWDSASSLLVSPILLFIGFVIVMPASILYRKKEVAESEVVKTEN"
     gene            2066..3187
                     /gene="dprA"
                     /locus_tag="VF870_06235"
     CDS             2066..3187
                     /gene="dprA"
                     /locus_tag="VF870_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX9251821.1"
                     /translation="MGKLTFEQLTDLFLLLSVDRIGPAKIRNLLARFKKLSNILSATT
                     SELIETEGISKELASRIRKISSQKDSVQKFLEKEIKVLEKMGGRIITVWDEEFPSILK
                     KIYDPPLVFYVLGQFDEKDKYSIAVVGTRQPTNYGKIQTEKIVADLVSQNITIVSGLA
                     RGIDSVAHSSALKNDGRTIAVIGSGLDVIYPAEHKKLFNEIKDKGVIISEFNLGTLPN
                     AENFPKRNRIISGLTLGTLVIETAITGGAMQTARLALDQNREVFALPGNLGVKQSEGT
                     NLLIQRGEAELVKSAEDILTELELKLKPVLGKNIPKQQKDLTIFEEKIYNCLLNEPLH
                     IDKLAEKTDLSTSDCLVNLLSLEFKGMVKQLPGKMFMLM"
     gene            complement(3189..>3938)
                     /locus_tag="VF870_06240"
     CDS             complement(3189..>3938)
                     /locus_tag="VF870_06240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9251822.1"
                     /translation="SILNRYRDKAAMGEKSFIGNILFKKFGEPTYISRLKIVDAGIND
                     SILDVGCGKGILLHKMKESGFNNVMGIDPFIDETISYPNGLKILKKNFNELEGKFDFI
                     MFNHSFEHMENPLEVMKQSNRLLNKNKYLLLRIPVADSYAFKHYRENWCSLDAPRHLF
                     LHTKKSIEVLAKHSGFEIKKINYDSRSWQLWGSEQYSKNISLMDERSYYINPKKSIFT
                     RNQIDEFEKQVVELNKNGEGDQAEFYLQKIN"
BASE COUNT         1330 a          643 c          641 g         1324 t
ORIGIN      
        1 gcgtaaaact gattcgtgat taaacagaat accgcttgag ataaacaagt tgtaggcttc
       61 acggtagtta acacatgtcc agtgtgcagc tgctttagaa attgcgtatg gacttgcggg
      121 atgaattata aaatcttcat caataggcag cttactttga gcaacattgc catacatctc
      181 actgctggaa gattgataga tttttatttt cttatcaacg atacgaattg cttctaaaat
      241 atttgcaacg gaaattatgt tatactcaag tgtaccgatc ggctgttcga atgataatcc
      301 aacagaactt tgtgcagcaa gattgtaaaa ttcatcaggc ttaaattttt ccaacaaacg
      361 gataatgttt gaaagatcaa aaacgttagc ttcaactaat tgaacatcgt tttcaacacc
      421 aagaaaattt aatccatttg aattaccgga ttgcagattt cttgtaattc caattacttt
      481 atattttttt tctaaaagat acttacttaa ataagctccg tcctgcccgg ttattcccgt
      541 aataagtgct gttttcatta atatttttct tattaaaccg tatgtagtga attaaatctt
      601 atcaaattta caacatcttt ttaagatatg gtaattagta agtcaactta cgcagaatta
      661 ggctttttgt tattccgaac ttgtttcgga atctcaattt gttaaaattg tagatgctga
      721 aatgaattca gcatgacaac tgcgtaaggt gtcttagtaa attcaatttt aagaaatggt
      781 acactgattt aactgattta ttatgatcat aactgatttt ataaaatcat aactatcagt
      841 gtactatttg tatttgagct ttacttccga tcaaaaaagc ttgacatttt acttttttct
      901 gtcaatattt gcatcagtaa taaacaaagg aaaaatatat aaccgatttg ctacattttt
      961 aattttcgtt cgtccaaata tttaattgtt agaaaattaa ccagagtagc aaatttaccg
     1021 atttagggaa tttctctggt cggttttttg ttatattaca aacactttaa acggagattt
     1081 attattcgaa ttgatacgat acttttagat atacgcaaac actaacatat aggagaaaca
     1141 cataatgaga gaaataagag caaaaataga caatccattg tctgaattga tcagcgatga
     1201 tatatttgat cttctagatt ctcatggtct tatagatgaa aaagctgtaa gggattatca
     1261 gataaggaaa aaatttaagc agcttagatc cggaaaacta agtgccggtg atgcaattga
     1321 tgcaattcgc gcagaatatc catatttaca atttgatacc attagaaaaa tagtatatca
     1381 gattagtaaa taattaatat taaataatac ttgacaaaga acatttatca aattattttt
     1441 caatcgactc tttcaaaggg ttctcccctg ccctttgata tgaatgagtc aggcccggtg
     1501 ataccccatc accgggtttc tattttaaac ctggagtact tatggcagta aaaactaaaa
     1561 agaagaatgt aaaaaccagt gtaaaagctc ttccatcacc ttttaatatt tattgggcaa
     1621 gaaccaatta tcttttattt ggattaggaa tgctgcttat aatttttggt ttttatttta
     1681 tgagtcttgg tgagtgggac agcgcgtctt cgcttttggt atctcccatt ttacttttta
     1741 taggatttgt tattgtgatg cctgcttcta ttctgtatcg taagaaagaa gtcgcagagt
     1801 cagaagttgt aaagactgaa aattaatttt ttttactgca atttattttt ccagattgtg
     1861 gaaataattt aatgtcattt cgaatccggc ttaagtcggt gagaaatctt ctataactaa
     1921 atattcagat ttctctcttc ggtcgaaatg actaacaaag cattaatcta atctgtcttt
     1981 taggaatgga aggcagaaaa aacttttaaa ccaattaaga ttatactttc aaatacttaa
     2041 tcattaatca attcttgcta aaaaattggg caaacttact tttgaacaat taactgatct
     2101 ttttcttctg ctttccgttg atcgaatcgg tcctgctaaa attagaaatc tacttgcacg
     2161 atttaaaaaa ttatcaaata tactttcggc tacaacttca gaattaattg aaactgaagg
     2221 aataagtaaa gaactagcct cccgaatccg gaaaatttca tcacaaaaag attctgtaca
     2281 aaaatttctt gaaaaagaaa taaaagtttt agaaaaaatg ggcggcagaa taatcactgt
     2341 ctgggatgaa gaatttcctt cgatattaaa aaagatttat gatccgcctt tagtgtttta
     2401 tgtgctcggt cagtttgatg aaaaagataa atactcgatt gccgttgttg gaacaaggca
     2461 accgactaat tatggtaaaa ttcagacaga aaaaattgtt gcagatttag tgagtcaaaa
     2521 tattactatt gtaagcggcc tggcacgtgg aattgattca gttgcgcatt catcagcatt
     2581 aaaaaatgat ggaagaacga tcgcagtcat cggatctgga ttagatgtta tttatcctgc
     2641 agaacataaa aagctgttta atgagataaa ggacaaaggt gtaataatct ctgaatttaa
     2701 tcttggaact cttccaaatg cagaaaattt tcccaagaga aacagaatca tttccggctt
     2761 aacacttgga acacttgtaa ttgaaacagc aattacaggc ggggcaatgc aaacagcaag
     2821 gttggctctt gatcagaata gagaagtttt tgctctcccc ggcaaccttg gtgttaaaca
     2881 atccgaaggt actaatttgc ttatccaaag aggcgaagca gaattagtaa aaagtgccga
     2941 agatatttta actgaacttg aattgaaatt aaaacctgtg ctcgggaaaa acattcccaa
     3001 gcagcaaaaa gatttaacaa tttttgaaga gaagatttat aattgtttac ttaacgagcc
     3061 tttgcatatc gataaactag ctgagaaaac tgatctttca acttcagatt gtcttgttaa
     3121 tttactttca ttagagttta aagggatggt aaaacaattg cccggtaaaa tgtttatgct
     3181 gatgtgagct aattaatctt ctgcaaataa aactctgcct gatcaccttc cccgtttttg
     3241 tttaactcca caacctgctt ttcaaactca tcaatttgat ttcgtgtaaa gatggatttc
     3301 tttggattaa tataatatga ccgctcatcc atcaaagaaa tatttttaga gtattgctca
     3361 cttccccaca attgccagct gcgtgaatca taatttatct tttttatttc aaatccggaa
     3421 tgctttgcta acacttctat tgatttcttt gtatgaagaa acaaatgcct tggtgcatca
     3481 agtgaacacc agttttctct gtaatgtttg aacgcataag aatcagccac aggaattcta
     3541 agcagaagat atttgttttt atttaaaagt ctgttcgatt gtttcattac ttcaagagga
     3601 ttttccatat gctcaaaaga atgattaaac attataaaat caaatttgcc ttctaattcg
     3661 ttaaagtttt tcttaagaat ttttaatccg ttaggatagc taattgtttc atcaataaaa
     3721 ggatcgattc ccataacatt attaaaaccg gactccttca ttttatgaag aagaatacct
     3781 ttgccgcacc caacatcaag aatagaatca tttattcctg catccacaat ctttaaacgc
     3841 gatatgtaag ttggctcacc aaactttttg aataaaatat ttccaataaa agatttttct
     3901 cccatcgcag ctttatcccg ataacgatta agtatgga
//
LOCUS       DASWBY010000332         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_15000, whole genome shotgun sequence.
ACCESSION   DASWBY010000332 DASWBY010000000
VERSION     DASWBY010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15000"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1176
                     /locus_tag="VF870_06245"
     CDS             103..1176
                     /locus_tag="VF870_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HEX9251823.1"
                     /translation="MVFIHLYLCDSSRRPATGFEDEIYVVADSSEFEELKVALQAAFE
                     VEINTPMPEKLFTLKRISSNLIEKVKNKKNIVIVAPLNSNSYTSQYIKSIVDSAIQKR
                     LETESDFILSKYDLWAKNQLVTVLSAANMQELEFKILKNKDNLLYAYQKISDKRLKES
                     LYSPAYERKAIEGLLLRDHGWLIYVQADYKLAKNDSVNNFVWLRRSPGSDMERWIFIH
                     WIDNANPEYLNPDSIKAIRNRLTKKYYQIRDDDSAYVVMVDSFYTTSEVNFNNRYALY
                     TQGLWDLNIKGMGGPFINYTFYDEKSKRVYMLDGSIYAPKYYKRNLIQQIDVILQSFL
                     TKDEISKDRAEDLIDDIDESIKY"
     gene            complement(1162..3108)
                     /locus_tag="VF870_06250"
     CDS             complement(1162..3108)
                     /locus_tag="VF870_06250"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX9251824.1"
                     /translation="MKYLHKYYFLLTGFISFVFYIITIAPSVIQIDTGELAAVQCTLG
                     IAHPTGYPLFTILGYFFSLIQLPFSKIFQMNLLAALYCSAAISVFTFTAKLVLDNLSE
                     FRFIKNAKEKSNRKKKDSAKNAQFVTASSFELSETTKIISAIFGGLFLALSKTFWFQS
                     TSVEVYSLHLMLITIIILALIKAFILSDKDNKISKYWIIFAVTLALGFTNHMTTLLII
                     PAVAYLYFVKNGFNSKSIKQIVFMLIIFFPILILFYSYMPIRASQSPLMNWGNPIDWE
                     RIIRHISGQQYQVWLFSSKEAAAKQFNYFIGNLPKEFSFSLIVSIAGLIVSFIYARKF
                     FIFNIIVFLSTVLYSINYDINDIDSYFLLAYISLAFFTVFGIVQMILFAANKKMEIIM
                     PVAVLVLLAGIQFYSNYDDVNQRNNFLYEDYTKSLLNTLPENSIVFSYQWDYFVSAAY
                     YFQLVENFRKDVAVVDKELLRRSWYYNQLNTNHPTLLNGVRSDVDNFLEALKPFEREQ
                     QYNANLLENLFRRIMTGLVSSNIDKHDYFIAPEIVDAEMKRSEFQLPPGYTLVPHLFL
                     FKVVNTNDYVEAPLPEFKIRFNKTEDKYVTSLKGFVASMLVRRAMYELQFNKSDRAKI
                     YAKKAAAEFPNITLPPVLSNLILN"
     gene            complement(3124..>3651)
                     /locus_tag="VF870_06255"
     CDS             complement(3124..>3651)
                     /locus_tag="VF870_06255"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251825.1"
                     /translation="AFTKCRFVSGNADLFKIFYTHYIQTVIGKDSTIIRKEMFEMRKK
                     LNPAIDSLFNIKKSSGGLADIDFIVSFYLLTNLDLLIVRNEKGIYNSFELMQKVSTKE
                     INFNQLEEYYYLLKKIELVNQLIFNNRLSKIPAEETKIKKLAKELGFSNSELFLNKLS
                     SIIEQTRKQYQKIFN"
BASE COUNT         1296 a          539 c          601 g         1215 t
ORIGIN      
        1 cttgaactaa agttagtttt aaaatatttc caataattac tttatgttta aatagttaaa
       61 gtttctaaat tttttggaaa tgatatttta tgaaacacaa aattggtatt tatacattta
      121 tacttatgtg attcatcaag aaggccggct accggatttg aagatgaaat ttatgtagtt
      181 gcagactcat cggaatttga ggaattaaaa gtagcactgc aggctgcctt tgaagttgaa
      241 ataaatacgc cgatgcctga aaagcttttt acattaaaaa gaatttcatc taaccttatt
      301 gaaaaagtta agaacaaaaa aaacattgta attgtagctc cgctaaactc taattcctac
      361 acttcacaat acatcaaatc gattgtcgat agtgcaattc agaaaagatt ggaaactgaa
      421 agcgatttta ttctaagtaa gtatgatttg tgggcaaaga atcagcttgt aactgtttta
      481 tcagccgcaa atatgcagga acttgagttt aagatcttaa agaacaaaga caatctatta
      541 tatgcatatc aaaaaatatc tgataaaaga ttgaaggaaa gtttatattc tccagcgtat
      601 gaacgcaaag ctattgaagg attgttgctg cgtgatcatg gctggttgat ttatgtacag
      661 gcagattata aactagcaaa gaatgattct gtaaataatt ttgtttggct gagaagatcg
      721 cctggcagcg atatggaaag atggattttt attcattgga ttgataatgc aaatccagaa
      781 tatttaaatc ctgattcaat taaggctatc agaaacaggt taactaaaaa gtattatcag
      841 attcgcgatg atgatagtgc ctacgttgtt atggttgata gtttttatac tacaagtgaa
      901 gttaatttta ataatcgtta tgctttgtat actcaaggtc tatgggatct aaacataaaa
      961 ggcatgggcg gcccatttat taattatact ttttatgatg agaaaagtaa aagagtgtac
     1021 atgcttgatg gttcaatcta tgccccaaaa tattataaac gaaaccttat tcagcagata
     1081 gatgttattc ttcaatcctt tttaaccaag gacgaaatct caaaagacag ggctgaagat
     1141 cttattgatg atatagacga atcaattaag tattaagttt gataaaaccg gcggcagggt
     1201 aatatttgga aattctgccg ctgctttctt cgcatagatt ttagccctat ctgatttatt
     1261 aaattgtaat tcatacatcg ctcttcttac aagcatagaa gcaacaaacc cttttaaaga
     1321 tgttacgtac ttgtcttctg ttttattgaa tctaatttta aactccggta atggtgcttc
     1381 aacgtaatca tttgtattta cgaccttaaa aagaaaaaga tgcggcacta gcgtataacc
     1441 gggaggcagt tgaaactcgc ttcgtttcat ttccgcatct acaatttcgg gagctataaa
     1501 ataatcatgc ttatcaatat tgctagacac caatccggtc ataattcttc tgaaaagatt
     1561 ttcaagaaga tttgcattgt attgctgctc tcgttcaaat ggttttaatg cttcaagaaa
     1621 attatcaaca tcagatctaa caccatttaa taacgttgga tgattagtgt tcaactgatt
     1681 gtaataccat gaacggcgca ataattcttt atctacaaca gcaacgtctt ttctgaaatt
     1741 ttcaactaac tgaaaataat atgcagctga aacaaaataa tcccactgat agctaaagac
     1801 tattgagttt tcaggaagag tgtttaacaa tgattttgtg tagtcttcat ataaaaaatt
     1861 attcctttga tttacgtcat cataatttga ataaaattgt atccctgcta ataaaacaag
     1921 aacagcgacc ggcattataa tttccatctt tttgtttgca gcaaacagaa tcatttgtac
     1981 aattccgaac acagtaaaaa aagcaagtga gatgtaagct aagaggaagt atgaatcgat
     2041 atcatttata tcatagttta ttgaatacaa tactgttgat aagaaaacta taatattaaa
     2101 aatgaaaaat ttcctagcgt aaataaacga aacaattaat cctgcaattg aaacaatcag
     2161 tgaaaatgaa aattctttag gaagatttcc aataaaataa ttaaactgtt ttgcagcggc
     2221 ctcttttgat gaaaaaagcc aaacctgata ttgctgtcca gaaatgtgtc ttattattcg
     2281 ctcccaatca atcggatttc cccaattcat caatggactt tgtgaagcac gaattggcat
     2341 gtaagaataa aacaaaatta aaataggaaa aaatattatc agcataaaaa caatttgctt
     2401 tatgctcttt gagttgaatc catttttaac aaagtacaag tatgcaacag ccgggataat
     2461 taaaagcgtg gtcatatgat ttgtaaatcc aagcgctaaa gtgacagcaa agattatcca
     2521 atatttagag atcttattgt ctttgtctga aagtataaag gctttgatca atgcgaggat
     2581 tataattgta atcaacatta gatgtaaact gtaaacttct acagatgtac tttgaaacca
     2641 aaatgtttta cttaatgcaa ggaacaatcc accgaagatt gcagaaatta tttttgttgt
     2701 ttcggatagt tcaaatgatg aagcagtaac gaattgagca ttttttgcag aatctttttt
     2761 ctttcgattg gatttttctt tagcattttt gataaaccgg aattcgctga gattatctaa
     2821 aactaattta gcagtaaaag taaaaacaga tattgctgca gagcagtaaa gcgctgcaag
     2881 taaattcatc tggaatattt ttgaaaaagg aagttgaatc agtgaaaaaa aatatccaag
     2941 aatcgtaaac aatggataac ctgtgggatg tgcaatgcca agtgtgcatt gaactgcagc
     3001 tagttctcca gtatcaattt gaattactga aggtgctatt gtaattatgt aaaatacaaa
     3061 tgatataaac ccggttaaga gaaaataata tttgtgtaag tatttcatat gctgtctgaa
     3121 aaattaatta aaaatttttt gatattgctt tcttgtctgt tctattattg aactaagttt
     3181 atttaagaaa agctcggaat ttgaaaaacc gagttcctta gcaagcttct ttatttttgt
     3241 ttcttctgct gggattttag aaagcctgtt attaaatatt agttgattaa ccaattcaat
     3301 tttctttagt aaataataat attcttcgag ttgattaaaa ttaatttctt tagtggaaac
     3361 tttttgcatt agctcaaaag aattatagat ccctttttcg tttctcacaa tcaataaatc
     3421 aaggtttgtc agcaaataaa atgaaacaat aaaatcaata tctgctaacc caccggagct
     3481 ttttttaata ttaaataatg aatcaattgc cggattaagc tttttacgca tttcaaacat
     3541 ttcttttctt attattgtcg agtcttttcc aatgactgtt tggatataat gagtatagaa
     3601 aattttaaac aaatctgcat tgccagaaac aaatcggcac ttagtaaaag c
//
LOCUS       DASWBY010000333         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_15018, whole genome shotgun sequence.
ACCESSION   DASWBY010000333 DASWBY010000000
VERSION     DASWBY010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15018"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VF870_06260"
     CDS             <1..365
                     /locus_tag="VF870_06260"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9251826.1"
                     /translation="GHSLGCVTALRYILEKNIKIKGAILVSGFINENPMDEQTEGLQE
                     FVDESLDIAKIKSLIPSRIVITAKDDDIVPTEATQKLSKALDANLIILSSGKHFIARD
                     GYTDFPILLNEIQKLSNT"
     gene            634..>1566
                     /locus_tag="VF870_06265"
     CDS             634..>1566
                     /locus_tag="VF870_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251827.1"
                     /translation="MNFGDILKNVAEKTLEYGEKGAKEVAKAGQAGYGKIEETVERAK
                     ENKQLQEETKKIVADTDRNWQDAVSRLEVAVEDTQEALEALEKQRIHIIEYQGARFQR
                     LYEYSLQLEEFSATANFSLESTNRNFADVQIGEHIYDNQTNPAVGGAAAGAVAAATAV
                     GATTFFGTAGTGTAIASLHGAAAINATLASLGGGSLAAGGLGMAGGMAVLGGLLIIPG
                     IAVGGYFWDKNVRQSYQKAVDYSEQVKESILKAKDIQSKYKRVSKTIQATIYETTALN
                     GFLDGLLNVFESDIITGISSESRKLCFDALTVSKR"
BASE COUNT          554 a          232 c          344 g          436 t
ORIGIN      
        1 tcgggcatag tcttggttgt gttacggcac tgcgctatat tctcgaaaaa aacataaaaa
       61 tcaaaggtgc aatacttgtt tcaggtttca taaatgaaaa tccgatggat gaacaaacag
      121 aaggtcttca agaatttgta gatgaatcac ttgacattgc aaaaataaaa agtctaattc
      181 catcaagaat tgttattact gcaaaagatg atgatattgt tccaaccgaa gcaacacaaa
      241 aattatctaa agcgttagat gcaaatctta ttattctttc atcgggaaag cattttattg
      301 ctcgtgatgg atatactgat tttcctattt tattgaacga aatccaaaaa ttgagtaaca
      361 cttaatattc taattgttaa tttccagata caagctgcat aatattttat ttaataacct
      421 cagtcgaaga ttcttcgaat cacatctgag cgccaataaa ttaccaagta caagcacctc
      481 actaagtaaa tatttagtga ggtgtttttt ttatacttag catttacaca agttataatt
      541 cccctttaaa atcatacctt tatatggtat aacaggagat agatagtgtt agggtggcta
      601 gacatcagat aaaaacgagg gagtgaaagc aatatgaatt ttggagatat tttaaaaaat
      661 gttgcggaga aaacattaga atatggagaa aaaggtgcaa aagaagtagc gaaggctggc
      721 caggcaggtt atggaaaaat cgaagaaact gtagaaagag ccaaagaaaa taagcagttg
      781 caggaagaaa ccaagaagat tgtagcagat acagacagga actggcagga tgcagtaagc
      841 cgtctagagg tagcggtaga agatacacaa gaagcactag aagcattaga gaagcaacgt
      901 atacatatta ttgagtatca gggtgcacga tttcaaagat tatatgaata ctcattgcaa
      961 ctcgaagaat tttctgcgac tgcaaatttt tcgctagagt ctactaatag aaatttcgca
     1021 gatgtgcaaa taggcgagca tatatatgat aatcaaacca atccagctgt tggaggagcc
     1081 gcggctggag ctgtagctgc tgcaacagca gtaggggcaa cgacattttt tggaacggct
     1141 ggaactggta ctgcaattgc aagtctgcat ggcgcagcag caattaatgc tacacttgcc
     1201 agtttaggtg gtggatcatt ggcggccgga ggcttgggaa tggctggagg aatggcggtg
     1261 ttgggtggtt tacttattat tccgggaatt gctgtaggcg gttatttttg ggacaaaaac
     1321 gtaagacaat cctatcaaaa agcggtggat tattcggaac aagtaaaaga atcaatttta
     1381 aaagcaaagg atattcaatc aaaatataaa agagtttcga agacaataca agccacgatt
     1441 tatgagacaa cagctttaaa tggtttttta gatgggttat taaatgtatt tgaaagtgat
     1501 attattacag gtataagttc agaaagtagg aagctttgtt ttgatgctct gactgttagt
     1561 aaaaga
//
LOCUS       DASWBY010000334         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_613278, whole genome shotgun sequence.
ACCESSION   DASWBY010000334 DASWBY010000000
VERSION     DASWBY010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613278"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2883
                     /locus_tag="VF870_06270"
     CDS             <1..2883
                     /locus_tag="VF870_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9251828.1"
                     /translation="IFLFAITIYSVSFAQPITVDPTTPTQYDSIVIFLNTTLPGATEL
                     LNYTGTVYAHTGVTTNLGIWQHVIGSWGQTNQPALTRLGPNLYKLTVGYPRTFYNVTN
                     PSEQITQLDIVFRSADGSKQTRPDIFIDLYEPGLTIVVQNPEVSVSFGDPQRSPAFVK
                     FGETVPITVNAVELGTKVSSLTISVDGNQVAQTNTKNLTYNFVHSNFSVGAHNIKVVG
                     TDTTGSVDSTSFMMFSNPAYTNAPLPDGLKQGINYTSVTTATLALYAPQKEYVYLLGD
                     FNDWKVETNYFLKRHFVNSDSVLWWIDINVSPGTEYAFQYLVDGKIRIGDPYSEKILD
                     PWNDQYIPAASYPNLKPYPAGKTESIVSVLQSAQPEYQWQVINFQKPPKEKLVVYELL
                     VRDFSTQSNYQFLIDTLSYLKSLGVNAIELMPVNEFEGNLSWGYNPSYHTALDKYYGT
                     PNKLKEFIDLCHQNGIAVISDIVLNHAFGLNPLVKMYFDGYYTIGSNSGIKTTPNNPY
                     FNVTSPNTTYFWGADFNHESPATQYYVDNVNRYWLEEFKFDGFRFDFTKGFTNTPGDG
                     GSYDQSRINILKRMADKIWEYDSTAYVILEHFTANSEEIVLSNYDMMIWGNHNCNYNQ
                     ATMGYASGPCGSWDFSGVSYKNRGWTKPNLVGYMESHDEERLMYKNLTYGNSSGSYNI
                     KNLNVALNRMKLAAAFFITVPGPKMIWQFGELGYDISIDSMGRVGNKPVKWDYLEDER
                     RTNLYKVFQSLIGLKQNYEAFSTNNFSLNVASSVKKMNLYHSTMDVFIIGNFDVIPLT
                     SSQNFSRAGWWYDYFSGDSVNVTNLTESITLEPGDFKIYTTTKLPTPEAGILLNVEKI
                     DDQIPVEFTLEQNYPNPFNPSTVIRYAVPNFTQGEIGGSRVILKIYDILGREIKTLVN
                     QEQTSGVYEVIWNGDDELGNKVSTGVYFYRIDAGEFVQTKKMMLIK"
     gene            3024..>3359
                     /locus_tag="VF870_06275"
     CDS             3024..>3359
                     /locus_tag="VF870_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX9251829.1"
                     /translation="MKRIKIFGREIIDEKSIAQIERCTSENDIGVLTADAHYGYGHPI
                     GGAVAYKNKISLSGVGFDIACGNKAVRTDIKADEMQLPKIMDEIYNQISFGIGRKNNQ
                     PVDHEVLDKI"
BASE COUNT         1140 a          555 c          632 g         1032 t
ORIGIN      
        1 atatttttat ttgcgataac gatttattct gtatcatttg cacaaccgat cactgttgat
       61 ccaacaactc ctacacaata tgacagtatt gtaatttttc ttaatacaac tttacccggg
      121 gcaacagagt tgctaaacta cacgggtaca gtatacgcgc atacaggagt tacaaccaat
      181 cttggtatct ggcagcatgt aatcggaagc tggggacaaa caaatcagcc tgcccttaca
      241 agacttggcc caaatttgta taagctaacg gtaggatatc ctagaacttt ttataatgtt
      301 acaaatcctt cagaacaaat tacacagctg gatatagttt ttagaagcgc agatggaagc
      361 aagcaaaccc gtccggatat ttttattgat ttatatgaac cgggcttaac aattgttgtt
      421 caaaatcccg aagtttcagt ttcgtttggc gatcctcaac gttcaccagc gtttgtaaaa
      481 tttggagaaa cagttccaat taccgtaaac gcagtagaac ttggtacaaa agtttcttcg
      541 ctaactattt ctgttgatgg aaaccaggtt gcgcagacaa acacaaaaaa cttaacatat
      601 aattttgttc actctaattt ttcagttggt gcacataata taaaagtagt tggtactgat
      661 acaaccggca gtgtggattc aacttcgttt atgatgtttt caaatcctgc ttacaccaat
      721 gcccctttac cggatggatt aaaacagggt atcaattaca caagcgtaac aaccgcaaca
      781 cttgcactat atgctcctca aaaagaatat gtttatcttc ttggagattt taatgactgg
      841 aaagtcgaga caaattattt tctaaagcgt cattttgtta attctgatag cgtattgtgg
      901 tggattgata ttaatgtaag tcctggaaca gaatacgcat ttcaatatct tgtagatgga
      961 aaaataagaa taggtgatcc gtactcggaa aaaattcttg atccatggaa tgatcagtat
     1021 attcctgcag catcataccc taatttaaaa ccttatccgg ccggtaaaac cgaaagcatt
     1081 gtaagtgtac ttcaatccgc tcaaccggaa tatcaatggc aggttattaa ttttcaaaaa
     1141 cctcctaaag aaaaattagt tgtctacgaa ctgctcgtaa gagatttttc tactcaaagt
     1201 aattaccaat ttttaattga tactctgtcg tatttaaaat cactcggtgt aaatgcaata
     1261 gaattaatgc ctgtaaatga atttgaagga aacttaagct ggggatataa tccaagctac
     1321 catactgctc ttgataaata ttacggcaca ccaaacaaac ttaaagagtt cattgatttg
     1381 tgtcatcaaa acggaatagc agttatatct gatatcgtat tgaatcatgc ctttggattg
     1441 aatcctcttg ttaaaatgta ttttgatggt tactatacta ttggtagcaa ttcgggtatc
     1501 aaaaccacac ctaataatcc atactttaat gtaacctctc cgaacacaac atatttctgg
     1561 ggtgcagatt ttaatcacga aagtcctgct actcagtatt atgtagataa cgtaaatcgc
     1621 tattggctag aagaatttaa gtttgatgga tttagatttg attttacaaa aggatttaca
     1681 aacacacccg gtgatggcgg gagctatgat caatcaagaa taaatatttt aaaaagaatg
     1741 gctgataaaa tctgggaata tgattcaaca gcttatgtga tacttgaaca ctttactgct
     1801 aatagtgaag aaattgttct ttcaaattat gatatgatga tctggggtaa tcataattgt
     1861 aattataatc aagctactat gggatatgct tcgggtcctt gtggcagttg ggatttttca
     1921 ggtgtatcat ataaaaatcg cggctggact aaacctaatt tagttggata catggaaagt
     1981 cacgatgaag aaagattaat gtataaaaat ttgacttatg gaaattcatc gggtagttat
     2041 aacattaaaa atcttaatgt agcattaaat agaatgaaac ttgctgctgc attttttatt
     2101 actgtgcctg gtcctaaaat gatctggcag tttggcgaac ttggttatga tattagtatt
     2161 gattcaatgg gtagagtcgg aaataagccg gttaagtggg attatttaga agacgaaaga
     2221 agaacaaatc tgtataaagt ttttcaatca ttaatcggac taaaacagaa ttatgaagct
     2281 tttagtacta ataacttttc acttaacgtg gcttcaagtg tgaaaaaaat gaatttatat
     2341 cattcaacta tggatgtttt tattatcggt aactttgatg taattccgct tacttcaagc
     2401 caaaacttta gccgcgctgg ctggtggtat gattatttta gtggtgatag cgttaacgtt
     2461 acaaacctga cagaaagcat aacattagag ccgggtgatt tcaaaattta tacaacaaca
     2521 aaacttccaa ctcccgaagc aggaattctt ctgaatgttg aaaagattga tgatcagatt
     2581 ccggttgagt ttactcttga acaaaactac cccaatccat ttaatccaag tacggttatt
     2641 agatatgcag taccgaattt cactcagggg gaaatcggag ggtcccgagt gattcttaaa
     2701 atatatgata ttctaggcag agaaataaaa acactcgtta accaggaaca aacaagcgga
     2761 gtctatgaag taatttggaa tggcgatgat gaactcggca ataaagttag cacaggagtt
     2821 tatttttatc gtattgatgc aggcgaattt gttcaaacaa aaaagatgat gctgattaaa
     2881 taattaacaa tcgtcacact gagcgcagtc gaagtgtgac ttcatcagat taattaaagg
     2941 cacgggcaat cgtgcctttt tttatagaat gacttagtta aataaaaaac ttataattga
     3001 tcaacatatt tctatcgaat attatgaaac gtataaaaat cttcggcaga gaaataattg
     3061 atgagaaatc aatcgcacag attgaaagat gcacatctga aaatgatatc ggtgtgctta
     3121 ctgctgatgc tcattacgga tatggtcatc cgatcggtgg ggcggttgca tataaaaata
     3181 aaatttctct atccggggtt ggatttgata ttgcgtgcgg caacaaagct gtaagaacag
     3241 acattaaagc agatgaaatg caactgccta aaattatgga tgagatttat aatcaaatat
     3301 cattcggcat cggcagaaaa aataatcagc cggttgatca cgaagtatta gataaaata
//
LOCUS       DASWBY010000335         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_726588, whole genome shotgun sequence.
ACCESSION   DASWBY010000335 DASWBY010000000
VERSION     DASWBY010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726588"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..709
                     /locus_tag="VF870_06280"
     CDS             122..709
                     /locus_tag="VF870_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX9251830.1"
                     /translation="MNKIIMHITYVSIITLFLFLSKSEASNENKGNGMEKNIKQIIVK
                     DIDGKSVKLSDYKGKVLLIVNVASYCGFTKQYAGLEEIYKKYKDKGFEILAFPCNQFG
                     EQEPGTNEEIKNFCLSKFDVTFKLFDKIDVNGKDKSPLYSILTDNEVTGKSDIKWNFE
                     KFLIDKDGNVVARYPSKVEPSSKELTSAIERELSN"
     gene            complement(803..1282)
                     /locus_tag="VF870_06285"
     CDS             complement(803..1282)
                     /locus_tag="VF870_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX9251831.1"
                     /translation="MLEYLRKIFSDNQPASIQNKVDSSLNQKNKKVEIAACALFIEMA
                     KADGEFTEDERNFIISEMKSTFNLDDDYANDLMILAEERVKESVSLYEFTGVINTTFT
                     HDEKIELIESLWKLIYNDAKLNQYEDHLIKRIAATINIEHKEIINAKLWVKQQLGLK"
     gene            complement(1287..1778)
                     /locus_tag="VF870_06290"
     CDS             complement(1287..1778)
                     /locus_tag="VF870_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9251832.1"
                     /translation="MDYELNPRTRIGHVHLTVANLDKALAFYRDLLGFQVTARFGKNA
                     VFLSSGNYHHHIGLNTWAGENATPAPEGHTGLYHFAILYPSREDLAKAFKRVWESNYP
                     IEGASDHGVSESIYIKDPDGNGLELYCDKLVEQWPKDEDGNLIMVTKPLDVPDLLSEL
                     DRM"
     gene            complement(1792..>2264)
                     /locus_tag="VF870_06295"
     CDS             complement(1792..>2264)
                     /locus_tag="VF870_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX9251833.1"
                     /translation="KFALNGALTIGTMDGANVEIREEVGDDNIFIFGLLADEVVKLKS
                     NGYNPKSYYEKNAELKHVVDMIATNFFSPNEFGIFDDMIRGLMEVDYYLLFADYQAYV
                     DAQDKVAQLYKNKEEWTKKSIYNVARVGKFSSDRSVKEYSDKIWKVKPVKVNHS"
BASE COUNT          735 a          418 c          339 g          772 t
ORIGIN      
        1 ggtcattcgc ctcttacatc ctgtcttgta atggatttag tccgcaataa ttaattatat
       61 tattttatat aattgaactt tttaactcta atcttagttt tcacctaaaa gaaagaataa
      121 aatgaacaaa atcattatgc atataactta tgtttcaata attacccttt ttcttttttt
      181 atcaaaatca gaggcttcaa acgaaaataa gggaaatgga atggaaaaaa atataaaaca
      241 aataattgtt aaagatatcg atggtaaatc tgttaagctt tcagattaca aaggcaaagt
      301 tttattgatt gtaaatgttg caagttactg cggattcaca aaacagtacg ctgggctaga
      361 agaaatttat aaaaagtata aagacaaagg atttgaaatt ttagctttcc cctgtaatca
      421 gtttggtgaa caggaaccgg gaactaacga ggaaatcaag aacttctgtt tatcaaaatt
      481 tgatgtaaca tttaaattgt ttgataagat tgacgttaac ggtaaagata aatcaccgct
      541 ttattcaatt cttacagata atgaagtaac aggcaagtcc gatattaaat ggaactttga
      601 aaagttctta attgataaag atggaaatgt agttgcaaga tatccaagta aagttgagcc
      661 atccagtaaa gagcttactt ctgcgataga aagagagctt agtaattagt cattgcgagt
      721 cccgaatttt cgggatgaag tagtcttcta ttacaaaact tatagatcac ttcacccgat
      781 aaatcgggtt cgtgatgaca aactatttca accccaactg ctgtttaacc caaagcttag
      841 cattaattat ttctttatgc tcaatgttta ttgttgcagc aatccttttt ataaggtgat
      901 cttcatactg atttaatttt gcatcattgt aaatcaactt ccaaagactt tcaatcaatt
      961 caattttctc atcgtgtgta aaagtagtgt ttatcactcc tgtaaactcg taaaggctca
     1021 cactttcttt cactctttct tcagcaagaa tcataagatc atttgcataa tcatcatcca
     1081 gattaaaagt agatttcatt tctgatatga taaaatttct ttcatcttct gtaaactcgc
     1141 catcagcttt tgccatttca ataaataaag cacatgcagc aatttcaacc tttttatttt
     1201 tttgatttaa ggatgaatcc actttattct gaattgacgc tggctgatta tctgaaaata
     1261 tttttcttaa atactccagc ataaaattac atcctgtcta attcacttaa caaatccgga
     1321 acatccaaag gttttgttac cattataaga ttaccatctt catcctttgg ccactgctca
     1381 actaatttat cgcagtataa ttcaaggccg ttaccatcag gatctttaat atatatcgat
     1441 tctgaaactc catgatcaga ggcgccttca atcggatagt ttgattccca aactctttta
     1501 aaagcttttg caagatcttc tctggatgga taaagaattg caaagtgata aagtccggta
     1561 tgtccttcag gagccggcgt agcgttttca ccagcccagg tatttaatcc aatgtgatga
     1621 tgataatttc ctgatgaaag gaatacagca tttttcccaa atctcgctgt aacctgaaac
     1681 cctagcaagt ctctgtaaaa tgctaatgct ttatcaagat tagctacggt taaatgaaca
     1741 tgtccaatac gtgtgcgggg atttaattcg taatccatta acttccaatt tttatgagtg
     1801 attaactttt accggtttaa ctttccagat tttatctgaa tattctttta ctgatctgtc
     1861 gcttgaaaac tttccaaccc gtgcgacgtt ataaatagat tttttcgtcc attcttcttt
     1921 attcttatac agctgtgcaa ctttatcctg cgcatctaca taagcctgat aatctgcaaa
     1981 caagagataa taatccacct ccatcaatcc gcgaatcata tcatcaaaaa ttccaaattc
     2041 attcggacta aagaagtttg ttgctatcat atcaacaacg tgtttaagct cagcattttt
     2101 ttcataatat gatttaggat tatatccgtt ggatttaagt tttactactt catcggcaag
     2161 caggccaaag ataaaaatgt tatcatcgcc aacttcttct cttatctcta cgtttgcgcc
     2221 atccatagta ccaattgtta atgcaccgtt aagcgcaaac ttca
//
LOCUS       DASWBY010000336         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_726628, whole genome shotgun sequence.
ACCESSION   DASWBY010000336 DASWBY010000000
VERSION     DASWBY010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726628"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF870_06300"
     CDS             complement(<1..458)
                     /locus_tag="VF870_06300"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9251834.1"
                     /translation="MKDIIPAEISVPEVQRLLQGGVAPRPIALVSTISKDGINNLSPF
                     SFFNIFGANPPVIAFSPSRRGRDSSLKDTYFNLIETKECVVQAVTFDMVEQVSLASTE
                     YEKGVDEFIKSGLTPIDSDLVKPKRVKESPFQMECKLLDMMSYGEGGASA"
     gene            complement(458..1474)
                     /locus_tag="VF870_06305"
     CDS             complement(458..1474)
                     /locus_tag="VF870_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9251835.1"
                     /translation="MKLVSYKEKKTDRVGIFYEGKVYDLQKSALSLKIKLPSTMKEFL
                     EGEKKSITLATKVFNAIKKGKVKSGLKHSRIRLLSPVPYPSSLRDGYAFRQHVATARR
                     NRGVEMIPEFDQFPIFYFGNHNTVFGEGEIKVENDHLEKLDFELECAVVIGKKGKNIS
                     VEDADSYIAGYLIMNDWSARVLQMEEMKLNLGPAKGKDFATSIGPWLVTPDELEKYKI
                     KTPFGDKYDLKMIARHNGNQISEGNLKDMNWTFAELIERASYGVTLFPGDVIGSGTVG
                     TGCYLELNGTWAIEAKETGKAFNPIWIKDGDDFELEITGLGVLKNKIKKSKTSRSILA
                     KKKI"
BASE COUNT          459 a          323 c          219 g          550 t
ORIGIN      
        1 ttagccgaag caccaccttc tccgtagctc atcatatcca aaagcttaca ttccatttgg
       61 aaaggtgatt ctttaactct cttaggtttt actaaatcag aatcaatcgg agttaagccg
      121 ctcttaataa attcatccac ccccttctca tattctgttg atgcaagact tacttgttct
      181 accatatcaa aggttacagc ttgtactacg cattctttag tttcgatcag attaaagtag
      241 gtatctttta gtgagctatc tctgcctctt cttgagggtg agaatgcaat taccggtgga
      301 ttagccccaa atatattaaa aaatgaaaac ggtgataaat tattaattcc atctttagaa
      361 atagttgaaa ctaatgcaat tggtcgggga gccacaccgc cctgtaataa tctttgaact
      421 tccggtactg atatctctgc aggaataata tccttcatta tatttttttc ttcgccaaaa
      481 ttgaacggct tgttttagat tttttaattt tattttttag aactccaagt ccggtgatct
      541 ctaattcaaa atcatctcca tcttttatcc aaatcggatt gaatgcttta cccgtttctt
      601 ttgcttcaat tgcccaggtt ccattcaatt ccagatagca tcctgttcct acagttccgc
      661 tgccaatcac atctccggga aaaagagtaa ctccataaga agcacgttca atcaattcag
      721 caaacgtcca gttcatatcc ttaagatttc cctcagaaat ctgattacca ttatgtctcg
      781 caatcatttt caaatcatac ttatcgccga agggagtttt aattttgtac ttttcaagtt
      841 catctggtgt cacaagccac ggcccgatag atgtagcaaa atctttacct ttagccggtc
      901 caagatttaa tttcatttct tccatttgca atactcttgc agaccaatca ttcattatca
      961 aataaccagc aatgtaggaa tcagcatctt caacagaaat gttttttcct ttcttaccaa
     1021 taactacagc acactccagt tcaaaatcaa gtttttctaa atgatcattt tcaactttaa
     1081 tttcaccttc tccaaaaaca gtattgtgat ttccaaaata aaatattgga aactgatcga
     1141 attccggaat catctcaact cccctgtttc tccttgctgt tgcaacgtgc tgccgaaatg
     1201 catatccatc gcgtaacgat gaaggataag gaacgggcga taaaagtcta attctgctat
     1261 gctttaatcc tgattttact ttaccctttt ttatagcatt gaaaactttt gtagctaaag
     1321 ttatagattt tttttcaccc tcaagaaatt ctttcatagt tgagggtaat tttattttaa
     1381 gcgatagagc cgatttttga agatcataaa ccttaccttc ataaaatatt ccaactctat
     1441 cagtcttttt ctctttatat gatactaatt tcatttaatg ttttccaata ttgttgtttt
     1501 attataatta aaaattattc ttatattata cggtaagagg gataaggtat a
//
LOCUS       DASWBY010000337         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_726720, whole genome shotgun sequence.
ACCESSION   DASWBY010000337 DASWBY010000000
VERSION     DASWBY010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726720"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VF870_06310"
     CDS             <1..807
                     /locus_tag="VF870_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase-like
                     domain-containing protein"
                     /protein_id="HEX9251836.1"
                     /translation="NINSQVFSEKDVEICNSKFKFAVDKSLSEKPINEVIIEIGKSFL
                     GTDYIAHTLEKEGDEQLVINLTGLDCTTFLETSLTLARCIKKGKTSFEDYQNELAFIR
                     YRDGKLDQYPSRLHYFSDWIYNNQQKGIVKDITKEIGGKEINFKVNFMSENPKYYKQL
                     KENPDFIPVISKQEKEINSRQYYYIPQEDIEKLESKIHSGDLIALTTSDKGLDIGHVG
                     LAVKMDDGRIHFLHAPLSGSKVQITEMPLSDYTKKIKKHTGIIVLRVLEP"
     gene            complement(808..>1736)
                     /locus_tag="VF870_06315"
     CDS             complement(808..>1736)
                     /locus_tag="VF870_06315"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX9251837.1"
                     /translation="YTRLTFIVQNSINKNKMKILITGGSGLLGQYLNSSLSSNHNVLS
                     LYYSNVGNCKNYNSLKINVCNYNSILTAFKDFRPDAVIHAAAMSHPILPAGISSKDVY
                     DLNVNSTKNIAELCDKFNSKLIYTSTDLVYAGYRGSMLKENAKLIPVSLYAETKLMGE
                     IKIQQTFDNYIILRTAILFGLGINHSKNHFHQMYLDLQNGNPVNLFTDQYRTPLSLNE
                     AARIINEIVSFDVKSEVINFGGLGRVSRYELGERLCEIAKLNKNLLVKIKMDDVPGLQ
                     KVEDVSMNTDKLQSLGIKQKSLDEMILEIVTK"
BASE COUNT          612 a          252 c          274 g          598 t
ORIGIN      
        1 aatattaatt cacaagtgtt ttctgaaaaa gatgtggaga tctgcaattc aaaatttaag
       61 tttgctgttg ataaatcatt aagtgaaaaa ccaattaatg aagttataat tgaaattgga
      121 aaaagttttc tgggaactga ttacattgct catacacttg aaaaagaagg tgatgaacag
      181 cttgttataa atttaaccgg acttgactgc actactttcc tcgaaacttc tttaacactt
      241 gcaagatgta ttaaaaaagg taaaactagt tttgaagatt atcaaaatga attggctttt
      301 attcgttatc gagatggaaa attagaccag tatccttcac gccttcatta tttttcagac
      361 tggatttaca ataatcagca aaagggaatt gtaaaagata taactaaaga aattggcgga
      421 aaggaaatta attttaaagt taatttcatg tctgaaaatc caaagtatta taaacaactg
      481 aaggaaaatc cggattttat tcccgttata agtaaacagg aaaaagaaat taattcccga
      541 caatattatt atatcccgca agaggatatt gaaaaattgg aatctaaaat tcattcgggt
      601 gatttaatcg ctttaactac aagtgataaa ggtttagata ttggccacgt aggattagcc
      661 gttaaaatgg atgatggaag aattcatttt ttgcatgctc ctttatcggg aagtaaagtg
      721 cagataaccg aaatgccatt aagtgattac actaagaaaa ttaaaaagca tacagggata
      781 attgtactta gagtattaga accataatca tttagtgaca atttctaaaa tcatttcatc
      841 aagcgatttt tgtttaatgc ctaacgattg aagcttatct gtattcatag aaacatcttc
      901 gactttttgt aaacccggta catcatccat tttaattttc actaaaagat ttttatttag
      961 tttagctatt tcacataatc tttcaccaag ttcatagcgt gaaactctcc ctaatccacc
     1021 aaagtttatt acttctgatt taacatcaaa gctaacaatt tcatttatta ttcttgctgc
     1081 ttcatttaag gaaagcggag ttctgtattg atcggtaaaa agatttactg ggtttccatt
     1141 ttgtaaatcc agatacattt gatgaaaatg atttttagaa tggttaatcc ccaacccaaa
     1201 taatatagct gttcttagta taatatagtt atcaaatgtt tgttgaattt taatttcacc
     1261 cattaatttg gtttctgcat aaagagacac cggaatgagt tttgcatttt cttttaacat
     1321 tgatcctcga taaccggcat acaccaaatc tgtagaagta taaataagtt ttgaattaaa
     1381 cttatcacaa agttcagcaa tattttttgt tgaattaaca ttaaggtcat aaacatcttt
     1441 tgaagaaata ccagccggta aaataggatg cgacatcgct gcggcgtgaa ttactgcatc
     1501 aggtctgaaa tctttaaaag cagttaagat tgagttgtaa ttacaaacat ttatttttaa
     1561 tgaattataa ttcttacaat ttccgacatt cgaatagtat aaactaagaa cattgtggtt
     1621 tgatgaaagg gaactattta ggtattgtcc aagtaatccg cttccgcccg ttattagtat
     1681 tttcatttta tttttattga ttgaattttg cactataaaa gttaaccgag tatatt
//
LOCUS       DASWBY010000338         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_275405, whole genome shotgun sequence.
ACCESSION   DASWBY010000338 DASWBY010000000
VERSION     DASWBY010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275405"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VF870_06320"
     CDS             <1..400
                     /locus_tag="VF870_06320"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9251838.1"
                     /translation="QSNLFYSIFDWITVTSNNGLVTLNGWVHLPWLRNQFQSEIEKIP
                     GVKSINNKIQNTFGPGELGVRAARLIYNDPMFHGLQYSVNPPIHIIVNNGTVILDGTV
                     DSEAESSWAANIINFHTDAFSVENNLQIRN"
     gene            450..968
                     /locus_tag="VF870_06325"
     CDS             450..968
                     /locus_tag="VF870_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251839.1"
                     /translation="MKKLRVLAFLFIISFYAAYANPVISPDNTLLKYYSALLTLNLNH
                     AMDASRIIYHLSMEPSFDKNFLENELNKIQQNVEYANTNIANIILNTLDNKKQEIDKP
                     LKNIDKHLAQVLVDIKEIRSKLKDSQSVTRDLSDIYFQINTSENDDHQEIKRILNLKT
                     DEEPLLVVPERL"
     gene            complement(1086..>2283)
                     /locus_tag="VF870_06330"
     CDS             complement(1086..>2283)
                     /locus_tag="VF870_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251840.1"
                     /translation="GFATYGEGVITEAWYGKTGYDNFIASEMAIARNAVGSIWVQDIS
                     QVWEIFNSNRTYAKGGVVLHMLRGIVGDSVFYDIMKTYSADPNVAYGVAVTEDFQAVA
                     ENVSGLDLNYFFQQWIYGENYPKYHSWFSKSLISGNTWNLSLLITQDANTNPSFFTMP
                     IQIKVTRAIGDTIVTVFNNAASQHYNISIKGEPLSIVFDPGNWILKTHSTIVPVELTS
                     FTAELNNQKVNLHWTTATEVNNQGFEIQRKSSKTDWAAIGFSEGRGTTTEQSNYSFVD
                     DISDMTQTNLYYRLKQTDFNGDFSYSDVVNVFLFPDDYSLTQNYPNPFNPSTLIKFSL
                     GKTGYTTLKLYDVLGKEVAALVNGELEAGPHEVTFDASSLPSGTYFYTLTSGSYTETR
                     KMLFLK"
BASE COUNT          809 a          399 c          341 g          734 t
ORIGIN      
        1 ccagtccaac cttttttact caatttttga ttggataaca gttacaagca ataatggttt
       61 agtaacttta aatggatggg tccatctgcc gtggttaagg aatcaatttc aatctgaaat
      121 tgaaaaaatt ccgggtgtaa aaagtattaa taataaaata caaaacacgt ttggaccggg
      181 tgaacttggc gtaagagcag caaggttaat atataatgat ccaatgtttc atggattgca
      241 atattctgtg aatccaccga tacatattat tgtaaataat ggaacagtaa ttttagatgg
      301 cacggttgat tcagaagcag agagcagttg ggctgcaaac attattaatt ttcacactga
      361 tgccttttca gtagaaaata atctgcaaat aagaaattaa gatttatgca gataactatt
      421 ttataaaatt tatttcaagg agatcaatca tgaaaaagtt aagagtatta gcattccttt
      481 ttattataag cttttatgca gcttatgcga atccggtcat ttcgccagac aatacgcttt
      541 taaaatatta ctctgcttta ctcacactta atttaaatca tgcaatggat gcttccagaa
      601 ttatttatca tctttctatg gaaccatcgt ttgataaaaa ttttttagaa aatgaattaa
      661 ataaaattca acaaaatgtc gagtatgcaa acactaatat tgcaaatatt attttgaaca
      721 ctttagacaa caagaagcag gagatagata aaccattaaa aaatatagat aagcacctgg
      781 cacaggttct tgtcgatatt aaagagatca gaagtaaatt aaaggattcc caaagcgtta
      841 ccagggattt atcagatatt tactttcaaa ttaatacttc agaaaatgat gatcatcagg
      901 aaattaaaag aatacttaat ctgaaaactg atgaggaacc tttgttagta gtgcctgaga
      961 gactttaata actaattctt ttaaagcatt tactttaatt ggattttata atcattcaca
     1021 ctttcaacta aaaaggcgat tcacacgaat cgccttaagt aaaaatcaaa atttaatttt
     1081 aaaaatcatt tcaaaaacaa catcttacga gtttctgtat agctgcctga ggtcagagta
     1141 taaaaataag tgccgctcgg taaacttgaa gcatcaaaag ttacttcgtg cggcccagct
     1201 tctaattcac cattaactag tgcagcaact tcttttccaa gaacatcata aagttttaaa
     1261 gttgtatatc ctgttttgcc taaactaaat ttaatcagcg tgctcggatt aaatggattt
     1321 ggataatttt gagttaaaga ataatcatcc ggaaacagaa aaacattaac aacatccgaa
     1381 tatgaaaaat cgccgttaaa atctgtttgc tttaatctgt agtaaagatt ggtttgcgtc
     1441 atatctgata tatcgtccac gaacgagtaa ttagattgct cagttgttgt ccctctgcct
     1501 tccgaaaacc ctattgcagc ccaatccgtt ttacttgatt ttctttgaat ctcaaatccc
     1561 tgattattta cttctgttgc tgttgtccag tgtaaattaa ctttttgatt attcaattct
     1621 gcagtaaaag aagttaactc aacaggcaca attgtagaat gagtttttaa gatccaatta
     1681 ccgggatcaa aaacaattga aagtggttcg cctttaatag aaatattata atgctgactt
     1741 gccgcattat taaatactgt aactattgta tcaccaattg ctcttgtaac ttttatctgg
     1801 ataggcatag taaaaaatga cggatttgtg tttgcatcct gagtaattaa aagtgataag
     1861 ttccaagtat taccgctgat taaacttttt gaaaaccatg aatgatactt tggataattt
     1921 tctccataaa tccattgctg aaagaaataa tttaaatcta aacctgaaac attttctgcc
     1981 actgcctgaa aatcttctgt tactgcaaca ccataagcta catttggatc tgcagaataa
     2041 gttttcataa tatcatagaa aacagaatct ccaacaatcc ctcttaacat atgcaaaacc
     2101 acaccaccct tagcataagt tctgttacta ttaaaaattt cccaaacctg agaaatatcc
     2161 tgaacccaaa tcgatccaac agcatttcta gcaattgcca tctcacttgc aataaaatta
     2221 tcataacccg ttttaccata ccacgcttca gtgataacac cttcaccata agttgcaaaa
     2281 cct
//
LOCUS       DASWBY010000339         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_275459, whole genome shotgun sequence.
ACCESSION   DASWBY010000339 DASWBY010000000
VERSION     DASWBY010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275459"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VF870_06335"
     CDS             complement(<1..789)
                     /locus_tag="VF870_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9251841.1"
                     /translation="MRKKFSYFSIVISLFLVSAGFAQTTTTTAKPDFPPSYYDIVAMF
                     LIAIIIISFIAIVYYEGRKGVAEEKEVLQKQPSAFARFRRYITKTTPVETEHELLLDH
                     EYDGIRELDSKIPPWFLWLFYLTIAFAIFYMLDYHVFNSSPLQAEEYQLQVKQAELER
                     AALINSGAFLNEESVTLLTDPTAIDAGKQIFATNCVACHAADGGGLVGPNLTDDYWIH
                     GGGIKNVFKTIKYGVTAKGMIAWQSQLNPKQMQDVASYVISLHGT"
     gene            complement(773..982)
                     /locus_tag="VF870_06340"
     CDS             complement(773..982)
                     /locus_tag="VF870_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEX9251842.1"
                     /translation="MYKELLQSIEGISLYPIISLIVFILFFAIILIWMFKVDNNYIKK
                     MENLPLEKDEENIFNNTGDLYEKKV"
     gene            complement(991..3111)
                     /gene="ccoN"
                     /locus_tag="VF870_06345"
     CDS             complement(991..3111)
                     /gene="ccoN"
                     /locus_tag="VF870_06345"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEX9251843.1"
                     /translation="MQLEKFSYDNKIVRDFTIASLVFGVVGMLVGVLIASQLFAPDLN
                     FSIPFLTFGRIRPLHTNAVIFAFVGNAIFAGIYYSLPRLLKAPMFSTLLSRIHFWGWQ
                     LIIVAAAISLPLGLTSSKEYAELEWPIDIAITLIWVVFGINMIGTIIKRREKHMYVAI
                     WFYLATWVTVAVLHIVNSFEIPVSLFKSYSLYAGVQDALVQWWYGHNAVAFFLTTPYL
                     GLMYYYLPKAANRPIYSYKLSIIHFWALIFLYIWAGPHHLLYSALPDWAQSLGTVFSI
                     MLIAPSWGGMINGLLTLRGAWDRVREDPILKFMVVAVTAYGMSTFEGPMLSLKNVNAI
                     AHFTDWIIAHVHIGALGWNGFLTAGMFYWLIPKIFKTELYSKKMANVHFWVGTLGMVF
                     YASAMYWSGVAQSLMWKQFNDMGMLQYPNFLETTLQIIPMYMIRAFGGTLYLVGMLLM
                     VYNLSKTMMKGKLVANEEAEAAPMHIEGKEKISHRWLEGKPIYFLLASLVVILIGGIV
                     EFVPTFLVKSNIPTIASVKPYTPLELQGRDLYIREACNSCHSQLVRPFRSETERYGEY
                     SKAGEYVYDHPFLWGSKRTGPDLHREGGKYPNLWHYLHMENPRSMSPGSIMPPYPWLL
                     ENTLDISLTSKKINVMRTLGVPYPDGYENQANDDLMKQAEAISLDLQKNGVPAEPDKE
                     IIALIAYLQRLGTDIKANPVTENK"
     gene            complement(3139..3339)
                     /gene="ccoS"
                     /locus_tag="VF870_06350"
     CDS             complement(3139..3339)
                     /gene="ccoS"
                     /locus_tag="VF870_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX9251844.1"
                     /translation="MVISVIVVLIGFSLLVAIGFLIAFLWSVKSGQYSDTYTPSVRML
                     FEDEKSKKEKKTEEETKIKEEN"
     gene            complement(3324..>3854)
                     /locus_tag="VF870_06355"
     CDS             complement(3324..>3854)
                     /locus_tag="VF870_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9251845.1"
                     /translation="VVKKLSPQYQLFLLSGDNNGEKDNLLKIFDDEAQLHFKQSPEDK
                     LNFIKQLQSNQTKVLMIGDGLNDAGALSQSDVGISVTDDISNFTPACDAILDSGQLKN
                     IASFLKFSKTSLKIIHLNFLISFFYNLVGLSFAINGMLSPLIAAVLMPVSSISVVLVA
                     TLGTNFIAKKRGLLSQ"
BASE COUNT         1269 a          732 c          626 g         1227 t
ORIGIN      
        1 agtgccatgt aaagaaatta catagcttgc aacatcctgc atttgctttg gatttaactg
       61 gctctgccag gcaatcatgc cttttgcagt tactccgtac tttattgttt taaatacatt
      121 tttaattccg cctccgtgaa tccaatagtc atcggttaga tttggaccaa ctaatcctcc
      181 accatccgca gcatgacaag caacacaatt agttgcaaag atttgtttac ctgcatcaat
      241 tgccgtcgga tctgttaaaa gagtaactga ttcttcattt aaaaatgcac ctgaatttat
      301 taatgctgct ctttcaagtt cggcttgttt aacttgtaat tgatattctt ccgcttgtaa
      361 tggacttgag ttaaaaacat ggtagtctaa catataaaat attgcaaagg ctatggttag
      421 atagaacaac cacaaaaacc atggtggaat cttgctatca agttctctga tcccatcgta
      481 ttcatgatct aataacaatt catgttctgt ttcaacagga gttgtttttg taatatatct
      541 tctaaacctt gcaaatgcag agggctgctt ttgtaatact tctttttcct cagcaacacc
      601 ttttctccct tcgtagtaca caattgcgat aaaactgatt ataataattg caataagaaa
      661 cattgcaaca atatcataat atgaaggtgg aaaatcaggc tttgcagttg ttgtagttgt
      721 ttgggcaaat ccagcagaga ctaaaaacaa acttataaca atcgagaaat aactaaactt
      781 ttttctcata taaatcacct gtattgttaa aaatattttc ttcatctttt tcaagtggaa
      841 ggttttccat ttttttaata taattattat caaccttaaa catccagata aggatgattg
      901 caaaaaataa aataaataca atcaaagaaa tgatcggata aagcgaaata ccttcaattg
      961 attgtaatag ttctttatac attacattac ctatttattt tctgtaacag gatttgcttt
     1021 gatatcagta cccagtcttt gcaaataagc aattaatgct atgatttctt tatccggttc
     1081 agcaggcact ccattttttt gtaaatccag tgaaatagct tcagcttgtt tcatcaaatc
     1141 atcattggct tgattttcat atccatctgg ataaggaacc ccgagcgttc tcattacatt
     1201 aatcttttta gaagttaatg aaatatccag agtgttttct aaaagccacg gataaggcgg
     1261 cattattgaa ccaggcgaca ttgaacgtgg gttttccata tgcagatagt gccataggtt
     1321 cggatatttt cctccttcgc gatgtaaatc gggtccggtt cgtttagatc cccacaaaaa
     1381 tggatgatca taaacatatt cacctgcttt agagtactca ccgtatcttt cagtttcaga
     1441 tctgaaagga cgaacgagct gcgaatgaca actgttacaa gcttctctaa tgtaaagatc
     1501 tcttccctgc aactcaagtg gtgtataagg ttttacagat gctattgtag ggatattgga
     1561 ttttactaga aatgttggaa caaactcaac aataccgccg attaagatta caactaggga
     1621 agccaatagg aaataaattg gtttgccttc caaccatcgg tgcgaaattt tttcttttcc
     1681 ttctatatgc atcggggcag cttcagcctc ttcatttgca actaatttgc ctttcatcat
     1741 agtctttgaa aggttgtaaa ccataagcag cattccaact aaatataatg ttccgccaaa
     1801 agctctaatc atatacattg gaattatttg aagtgtggtt tccaaaaagt taggatactg
     1861 cagcattccc atatcattaa attgtttcca cattaatgat tgagcaacac cagaccaata
     1921 cattgctgat gcgtaaaata ccattccaag cgttccaacc cagaaatgga cgttagccat
     1981 ttttttagaa tatagttctg ttttaaatat ttttggaatc aaccaataga acattccggc
     2041 tgttaaaaat ccattccacc caagtgcacc aatgtgtacg tgagcaataa tccaatcagt
     2101 aaagtgcgca atggcattta catttttaag agaaagcatt ggaccttcga atgttgacat
     2161 accatatgct gttacggcaa caaccataaa tttaaggatt ggatcttctc ttactctatc
     2221 ccatgccccg cgtaaagtta aaagtccgtt tatcattcca ccccaggaag gagcaattaa
     2281 cataattgag aaaactgttc caagtgactg ggcccaatct ggtaaagcag aatataataa
     2341 gtgatgagga ccagcccaga tatataaaaa tatcaaagcc caaaagtgta tgattgaaag
     2401 tttgtaagaa taaataggtc tattggcagc ttttggcaga tagtaataca tcaaacctaa
     2461 ataaggagtt gttaagaaga atgcaacggc attatgccca taccaccact gtaccaaagc
     2521 atcctgaaca cctgcataca atgaataact cttaaataaa cttacaggaa tttcaaaaga
     2581 atttacaata tgcaatactg caacagtaac ccaggttgca agataaaacc atattgcaac
     2641 atacatatgc ttttctcttc tcttgattat agtgccaatc atattaattc caaagacaac
     2701 ccatataaga gttatagcta tgtcaattgg ccattcaagt tcagcatatt ctttactcga
     2761 tgtaagacct agtggaagtg agattgctgc ggctacaatt attaattgcc atccccagaa
     2821 atggattctg ctgagcaatg tgctaaacat tggtgcttta agaagtcgtg gaagcgaata
     2881 ataaatgcca gcaaaaatcg catttccaac aaaagcaaaa ataacagcat tggtatgcag
     2941 aggtctgatt cttccgaaag ttaaaaacgg aatactaaaa ttcagatcag gggcaaaaag
     3001 ctggcttgca ataagcacac caacaagcat acccacaaca ccaaatacca acgatgctat
     3061 agtaaagtct cttacgattt tattatcgta gctgaacttc tctaactgca taagggctcc
     3121 tttaattgaa gtggtagttt aattctcttc tttaattttt gtttcttctt cagttttttt
     3181 ttcttttttt gatttctcat cttcgaaaag catgcgcaca gatggagtat atgtatctga
     3241 gtactgcccg cttttaacac tccacaagaa agcaattaga aatcctatcg ctacaagtaa
     3301 actaaatcca attaagacaa cgattactga gataacaatc ctcttttttt agcgataaaa
     3361 tttgttccta atgttgcaac taaaactaca ctaattgaac ttactggcat cagcactgca
     3421 gcaattaacg gggaaagcat tccattaatt gcaaaactta gtccgactaa attataaaaa
     3481 aatgatatta aaaagtttag atgtataatt tttaaactgg ttttagaaaa ttttaagaag
     3541 cttgcaatat tttttaattg ccctgagtca agaattgcgt cacaagcagg agtaaaatta
     3601 cttatatcat ccgttactga aataccaaca tcgctttggc taagtgcacc agcatcgttt
     3661 aatccatcac caatcattaa tacttttgtt tggttagact ggagttgctt aataaaattc
     3721 aatttatctt caggtgattg tttaaaatgc agttgtgctt catcatcaaa gatttttaat
     3781 aagttatctt tctcaccgtt gttgtctcct gaaagcaaga aaagttgata ttgaggtgat
     3841 aattttttaa caac
//
LOCUS       DASWBY010000340         3546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_500695, whole genome shotgun sequence.
ACCESSION   DASWBY010000340 DASWBY010000000
VERSION     DASWBY010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500695"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /locus_tag="VF870_06360"
     CDS             <1..2025
                     /locus_tag="VF870_06360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HEX9251846.1"
                     /translation="KINRSLMKRIILVFTWIGFISVFAQSPLEKIAPRLQLKMQSDVQ
                     SDKYLVWVYFTDKGNNIDAYYSNPQSVVSKKSLDRRAKVLSASNIINYSDIPVNQNYI
                     TLLTARGFELKHKSKWFNAVSGYATQSQINQIASNSFVGKIDVVGAFTKNYDDVEFNS
                     NQTFVENLTQPEGTHVLNYGSSYTQLQLSNVPAVHDLGFTGLGVTICLMDAGFDNLPH
                     EVFSNMDIIAAYDFVNNDPNVGNQGDMGEGSHGTATLSLIGGFKDGQMIGPAYGSNYI
                     LAKTENTDSETPVEEDNWVAALEWADSIGVDVTSTSLGYIVYDPPFTGYTWEDMDGNT
                     ALITRAGDLAAAKGIVVVNSAGNEGNNTSHNTLGAPADGDSIFAIGSVNSSGLRSSFS
                     SVGPTVDGRIKPDFMAQGSNDYHASSFGNSYSYGSGTSFSCPIAAGICALMLQKNPNL
                     TPMEVRELLRSASSNSGSPNNQMGWGIINALSAINLVTVPVELTLFTANYEDGVVNLK
                     WVTATETNNYGFEVERKNNNSAYGSVGFVSGTGTTTNRVTYNFTDQNLSNNRYYYRLK
                     QMDFDGSFEYSNEVQVDIIDLNDFQLYQNYPNPFNPSTKIKYTIPKNSFVKITLHDIL
                     GNEIRTLVDNSVQEGTYEIQLDGSNLSSGMYFIRLSSENVQRTVKISLIK"
     gene            2143..>3546
                     /locus_tag="VF870_06365"
     CDS             2143..>3546
                     /locus_tag="VF870_06365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9251847.1"
                     /translation="MSLRISFLFAALLAFMFVSCSPEHSKIIIAQYDDSKITMDEFEK
                     AYAKNVGGIEAAVKDSFDNYKNFADLYTNFKMKLDDAKVRGYQNDPSLNDELMDYKKK
                     VGASYILEKYLVEPNIKDWYEKRKTEIRASHLMIRVDSTGEAAAAKYAQSILDSIKNG
                     KSFEEMVSKYSQDQFSKPKGGDIFYFTAGQLPFEFEDACYKTEKGTVYPEVVKTRFGY
                     HIIKVTDKKPRVPKIRASHILISYMNGAGVVDTAAAELTMDSVLTELKAGKDFGEVAK
                     RYSDDTGTKDKGGDLGFFERRMMVQEFDEAAFNLEVGEVSDVVKTNFGLHVIKVTEKE
                     EYPTFEADRENLKNLLKRSRYNDLYAELIDSYKKEYNYNVNETTIQQMVGYNDSTVIG
                     GEMKGLSEIGDKTVFSFDNHTESVNDFYSKIKSDTEFSGKKFTVDILNKGVKKLSEQL
                     LLEEKALSLEKTDAQFAD"
BASE COUNT         1239 a          518 c          686 g         1103 t
ORIGIN      
        1 aaaattaata gaagtttgat gaaaagaatt atccttgtat ttacttggat tggatttatt
       61 agtgtttttg cgcaaagtcc gcttgaaaaa attgctccaa gacttcagtt aaaaatgcaa
      121 tcagatgttc agtctgataa atatttagtc tgggtttatt ttactgataa agggaataat
      181 attgatgcat attattcaaa cccacaaagt gttgttagta aaaaatccct tgatagaaga
      241 gcaaaagttt tatcggcatc aaacataatt aattattcgg atataccagt taatcaaaat
      301 tacattactt tattaactgc aagagggttt gagcttaaac ataaatcaaa atggtttaat
      361 gcagtaagtg gttatgcaac tcagagtcag ataaatcaaa ttgcttcaaa ttcatttgtt
      421 ggtaagatag atgttgttgg tgcttttact aaaaattatg atgatgtaga attcaattca
      481 aatcaaacat ttgtggagaa tctaactcaa cctgaaggta ctcacgtttt gaattatggt
      541 tcatcctaca ctcaattaca attaagcaat gtaccggcag ttcacgattt aggatttaca
      601 ggtttaggtg ttacaatttg ccttatggat gcaggttttg ataatcttcc acacgaagta
      661 ttttctaata tggatattat tgccgcgtat gattttgtta ataatgatcc caatgtaggc
      721 aatcaaggag atatgggaga aggatctcat ggaactgcga ctttatcatt aattggcgga
      781 ttcaaagatg gccaaatgat tggcccagca tatggctcca attacatatt agcaaaaact
      841 gaaaatactg atagcgaaac tcctgttgaa gaagataatt gggttgcggc tctggaatgg
      901 gcagacagca tcggcgttga cgtaacctca acatcactgg gttatatcgt ttacgaccct
      961 ccatttactg gttatacctg ggaagatatg gatggaaata ctgctttgat tacccgggca
     1021 ggtgatcttg ctgctgcaaa aggaatagtg gttgtcaatt ccgctgggaa tgaaggaaat
     1081 aataccagcc ataatacttt aggcgcacct gctgatggtg atagtatttt tgctattggt
     1141 tctgttaata gctccggttt aagatcatca tttagctcag ttggaccaac tgttgacgga
     1201 agaattaagc cagactttat ggctcagggt tcaaacgatt atcatgcctc atcttttgga
     1261 aattcttata gttatggaag tggaacttca ttcagttgtc ctatcgctgc aggaatttgt
     1321 gctcttatgc tccagaaaaa ccctaactta actccaatgg aagtgagaga attgttaaga
     1381 tcagcatcct caaacagcgg tagtccaaat aatcaaatgg gatggggaat aataaatgca
     1441 ttatccgcta tcaatttagt aactgtgcct gttgaactga ctttatttac tgcaaattac
     1501 gaagatggag ttgtaaattt aaagtgggtt accgcaacag aaacaaacaa ctatggattt
     1561 gaagttgaaa gaaaaaacaa taactcggct tatggatccg ttggatttgt aagtggcacc
     1621 ggaactacaa cgaatagagt tacatataat tttaccgatc aaaatttatc aaataacaga
     1681 tattattaca gattgaaaca aatggatttt gatggatcat ttgaatactc aaatgaagtt
     1741 caagtggata ttattgattt aaacgatttt caactttatc agaactatcc caatccgttt
     1801 aatccatcaa caaaaataaa gtacactatt ccaaagaata gttttgttaa gataacatta
     1861 cacgatattt taggtaatga aattagaacg ttagttgata attcagttca agagggcact
     1921 tacgaaattc aacttgatgg ttctaatcta tcaagtggaa tgtattttat tagattgagt
     1981 tcagagaatg ttcagagaac tgtaaagatc tctttgatta agtagttttt aaggctgata
     2041 cgtccgaaaa gttgatgtat cagcctttaa ttaccttgat tttctaaccc tctgtttata
     2101 aaattcaaag ttaaatttgt aacaattata caggagataa taatgtcttt aaggatttct
     2161 tttttatttg ccgccttatt agcatttatg tttgtttcat gttctccgga acactcgaaa
     2221 ataataattg ctcagtacga tgattccaaa atcacaatgg atgaatttga aaaagcttac
     2281 gcaaaaaatg ttggaggaat tgaagctgct gttaaagaca gctttgataa ttacaaaaat
     2341 tttgctgatt tatatactaa tttcaaaatg aagctggatg atgctaaagt tcgcggctac
     2401 caaaatgatc cttcacttaa tgatgaactg atggattata aaaagaaagt tggagcatct
     2461 tatatacttg aaaaatacct tgttgaacct aatatcaaag actggtacga aaaaaggaaa
     2521 acagaaattc gtgctagcca tcttatgatt agagttgatt cgaccggcga agctgctgct
     2581 gcaaaatatg cacaatcaat tcttgacagc ataaaaaatg gtaaaagttt tgaagagatg
     2641 gtatcaaagt attcccagga tcaattttca aaaccaaagg gcggggatat attttatttt
     2701 actgccgggc agctgccatt cgagtttgaa gatgcatgtt ataaaactga aaaggggact
     2761 gtttatcctg aagtcgtaaa aaccaggttc ggttatcata tcattaaagt aacagataag
     2821 aaaccccgag ttccaaaaat aagagctagt catatactta tcagttacat gaatggtgct
     2881 ggtgtggttg atactgctgc tgcagaacta acaatggaca gtgtgttaac cgaattaaaa
     2941 gccggtaaag attttggaga agtagctaaa agatattcag acgacacagg aacaaaagat
     3001 aaaggtggtg atcttggatt ctttgaacga agaatgatgg ttcaggaatt tgatgaagca
     3061 gcatttaatt tagaagttgg agaagtatct gatgttgtta aaacaaattt tggtttgcat
     3121 gtaattaaag tgacagaaaa agaagaatat ccgacatttg aagctgacag agaaaatctt
     3181 aaaaatttat taaaacgatc tcgttataat gacttatacg cagaattaat tgatagttat
     3241 aaaaaagaat acaattataa cgtaaatgaa actactatcc agcaaatggt tggatacaat
     3301 gattccactg ttattggcgg tgagatgaaa ggattatctg agattggcga taaaacagtt
     3361 tttagttttg ataatcatac agaatcagta aatgattttt attctaaaat aaaatcagac
     3421 acagaattta gcggtaaaaa gtttacagtg gatattctta ataaaggtgt taagaaatta
     3481 agcgaacaat tactgctgga agaaaaagca ttatcacttg aaaaaaccga tgctcaattt
     3541 gcagat
//
LOCUS       DASWBY010000341         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_88172, whole genome shotgun sequence.
ACCESSION   DASWBY010000341 DASWBY010000000
VERSION     DASWBY010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88172"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..773
                     /locus_tag="VF870_06370"
     CDS             63..773
                     /locus_tag="VF870_06370"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX9251848.1"
                     /translation="MEENKNKVDKRVLLGSILIGLGGLFFLNSLDIFDFNFGRVIFSW
                     PFFFIVIGMYLTLNTSRKMLGGILAGLGFIFILPRIFPSINYDGSVVFAIFLIAIGSY
                     IILNQKKKTEQTDELGQITRDVIDDVAIFGGGTKIVSSDNFKGGNITAVFGGSEIILK
                     GCKLAEGTNSIDILAVFGGTTIVVPNEWNVVMNVTSIFGGFSNKSVKDPNAFVDQSKT
                     LIIKGLVVFGGGELKTYL"
     gene            783..1844
                     /locus_tag="VF870_06375"
     CDS             783..1844
                     /locus_tag="VF870_06375"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9251849.1"
                     /translation="MSINPILKSPKSILLYLLFVLAIAVLYVNLITLEGKASLTISIA
                     DAIVFNLLIAALGLSFWYASVYLSIEHNQITKVILTHFGGGLLISIVWLALGYFAVIS
                     IVNDTDMFGNFFVTTIKSRFIIGILYYFLLTAFYYIVIYYSGFQERTLKETELKNLVT
                     EAELRSLKFQINPHFIFNSLNSMSALTEIDPKKAKQMIIKLAEFLRYILANNDRQKNT
                     LNEELKNIKLYLEIEKIRFDDKFIYVEELHEECGKTEIPSMILQPLFENAIKHAVYET
                     LDTVTIKLTCSRQDDFLKITLCNNFEGESHKKGAGVGLKNIKDRLSLIYHQDNLMEIK
                     KESGKFIVSIYIPLQKVLA"
     gene            1848..2597
                     /locus_tag="VF870_06380"
     CDS             1848..2597
                     /locus_tag="VF870_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEX9251850.1"
                     /translation="MTNKIKIIIVDDESLARQITKNYLASRNDVEILAECSNGFDAIK
                     RINELKPDLIFLDIQMPKLNGFEMLELLEDPPIIIFTTAYDHFAIKAFEVSAVDYLLK
                     PFSEERFTSALQKAIAQLGDRFQQNSAIQNIIKQRDEKIEFLERVVIKDGGKITIIPV
                     EEVKWIEAQDDYVLINSEKGRFLKQKTMKYFENHLNENQFVRIHRSYIINLDFLQHLE
                     QTDTESYRLVLKNGKELPVSKTGLTKLKSTL"
BASE COUNT          983 a          369 c          456 g          928 t
ORIGIN      
        1 tatcattatc tttgtattag ttttccacca gaaaacggta tatctattta aggagcagaa
       61 aaatggaaga aaataaaaac aaagttgata aaagagtatt gcttggaagc atacttatcg
      121 gattgggcgg tttattcttt cttaattcat tagatatttt tgattttaat tttggaagag
      181 taatcttttc ctggccattc ttttttattg taattggaat gtatcttaca cttaacactt
      241 caagaaaaat gcttggcgga attcttgcag gtcttggttt cattttcatt cttccaagga
      301 ttttcccatc aattaattac gatgggtcag ttgtttttgc aatattttta attgctatcg
      361 gcagttatat aatacttaat caaaaaaaga aaactgaaca aactgatgaa ctaggacaga
      421 ttacacgtga tgttattgat gatgttgcaa tttttggcgg aggaactaaa attgtaagtt
      481 ctgataattt taaaggaggt aatattacag cagttttcgg cggatcagaa attattctta
      541 aaggctgtaa acttgctgaa ggtacaaact caatcgatat ccttgcagtt tttggcggaa
      601 ctactatagt cgttccaaat gagtggaatg ttgtaatgaa tgttacttca atctttggcg
      661 gtttctcaaa taaaagcgtt aaagacccta atgcattcgt ggatcaaagt aaaactttaa
      721 taataaaagg cctggttgtt tttggcggcg gtgaattgaa aacatatttg taataatcat
      781 atatgtctat taacccgata ttaaaaagtc ctaaaagtat tttactatac ctgctcttcg
      841 ttttagcaat tgctgtactt tatgtaaatc ttattacact tgaaggcaaa gcttcattga
      901 caatatcgat tgctgatgca attgtattta acctgttaat tgctgcactc ggtttaagtt
      961 tctggtacgc ttcagtatat ctttccatcg aacataacca aataacaaaa gttatactta
     1021 cgcattttgg cggcggatta ttaatctcaa tagtatggct tgcactggga tattttgcag
     1081 ttattagtat tgtaaatgat actgatatgt ttggcaattt ttttgttacc actattaaat
     1141 caagatttat tattgggata ctttattatt ttctgttaac tgcgttctat tatatcgtaa
     1201 tttattattc cggatttcaa gaacgaacgt tgaaggaaac tgaattaaaa aatcttgtta
     1261 ctgaagcaga attaagatcg cttaagtttc agataaaccc gcatttcatt tttaacagct
     1321 taaattcaat gagtgcgtta actgaaattg atcccaaaaa agccaagcaa atgattatta
     1381 agttggcaga atttttacga tatattcttg caaataatga cagacaaaaa aacacactta
     1441 acgaagaact aaaaaatatt aaactgtatc ttgagataga aaagatacgt tttgatgata
     1501 agtttattta tgttgaagag ttacatgaag aatgcggtaa aacagaaatt cctagcatga
     1561 ttcttcagcc gctttttgaa aatgcaatta aacatgcagt gtatgagact cttgatactg
     1621 tcacaattaa attaacttgt tccaggcagg atgatttttt aaagattact ctttgcaata
     1681 attttgaagg agaatcacat aaaaaaggtg ctggagttgg tttaaagaat attaaagata
     1741 gattatcatt aatttatcat caagataatc ttatggaaat aaaaaaagag agtggtaaat
     1801 ttatagtgag tatttatatt cctttacaaa aagtactggc ttagactatg acaaataaaa
     1861 taaagataat tattgttgat gatgaatcac ttgcaagaca aattacaaaa aactatctgg
     1921 caagtagaaa cgatgttgaa attcttgcag aatgttcaaa tggatttgat gcaataaaga
     1981 gaattaatga attaaagccc gatctgatat ttcttgatat tcaaatgccg aagttaaatg
     2041 gatttgaaat gcttgaactt cttgaagatc caccgataat tatttttact acggcttatg
     2101 atcattttgc gataaaagct tttgaagtta gtgctgtgga ttatttatta aagccatttt
     2161 cagaagaacg ttttacaagt gcattacaaa aagcaattgc acaacttggt gatagattcc
     2221 agcaaaattc agcaattcaa aatatcatca aacagcggga tgaaaaaatt gaatttcttg
     2281 aacgtgttgt tattaaagat ggtggaaaga ttactataat tcctgttgaa gaagtaaaat
     2341 ggatagaagc acaggacgat tatgtgctca tcaattctga aaaaggaaga tttctaaaac
     2401 aaaaaactat gaagtatttt gaaaatcatc ttaatgaaaa tcaatttgtc cgcattcata
     2461 gatcttatat tataaactta gattttcttc agcatcttga acaaacagat acagaatctt
     2521 atcgattagt tttaaagaat ggcaaagaat tgcccgtaag caaaaccgga ctgacaaaac
     2581 taaaatcaac attataaact catttaagtc taaagtatta ttaattaaca tatttcttga
     2641 ctttaaacaa aattcaagtt aagtttttaa cgtttgaaaa atttcaaaag ttaaaatcag
     2701 tatgataacc gaagataaaa tcaaaaaaga acttat
//
LOCUS       DASWBY010000342         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_199020, whole genome shotgun sequence.
ACCESSION   DASWBY010000342 DASWBY010000000
VERSION     DASWBY010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199020"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1149)
                     /gene="mraY"
                     /locus_tag="VF870_06385"
     CDS             complement(40..1149)
                     /gene="mraY"
                     /locus_tag="VF870_06385"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX9251851.1"
                     /translation="MLYYLFEYINKTFNPPGFDIFRFLTFRSALAAITGLFLSLYLGP
                     KIIKKLQVHQIGEAKKADGPKFHWSKVGTPTMGGLIIVLSVTIPVLLWGDLKSTYIIL
                     ILVGTLWLSAVGFLDDYLKVIKKYPNGLIARYKLMGQVLIGLVVGSVIYFSPEFHGVS
                     TLTTVPFLKNVNLDFSILYIPLVVFIITATSNAVNLTDGLDGLATGVIVIVMIALALL
                     SYFSGNTIFANYLNIIHLPGSGELTVFIAALVGAGLGFLWFNTYPAQVFMGDTGSLAL
                     GGAFGIIAVLIKKELFIPILGGIFFMETLSVIIQRLYFKYTKKKFGEGKRVFKMAPMH
                     HHFEMVGWPEPKIVVRAYIIAIILAIVTLVSFKIR"
     gene            complement(1150..>2012)
                     /gene="murF"
                     /locus_tag="VF870_06390"
     CDS             complement(1150..>2012)
                     /gene="murF"
                     /locus_tag="VF870_06390"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX9251852.1"
                     /translation="PYTAPIASPDFGLITNIGDSHLEYLKNRKGVFKEKSALLHETIK
                     RGGKVFINNNDKILKEYGKTVKNKITYSLNEKADFKVSIKGYDKVARPQIEIKNKKNS
                     LTVALPISGEKNVLNFTVAYAIASELGLTSTQILKAVKKLKSYNKRLEIKNYKKFTVI
                     NDTYNANPDSMKSSIEILNKIKSRKRKIAVLGDMFELGNESKSKHLDLANFINKTKVD
                     EVYSIGKMMKFMNQKLNGKTKVHKHFSNRESLKKYLQASVINNSVVLIKGSRGMKMEE
                     FVSVIESAKN"
BASE COUNT          662 a          364 c          274 g          712 t
ORIGIN      
        1 ccaattattg atattttttt attttttaat tctaacatct tatctgatct taaatgaaac
       61 taaagtaacg attgcaagaa taattgcaat tatgtatgca cgcacaacta tttttggttc
      121 cggccaacca accatttcaa aatgatgatg cattggcgcc attttaaaga cacgtttacc
      181 ttcaccaaat tttttctttg tgtacttgaa gtaaagtctt tgaattatca ctgacaaagt
      241 ttccataaaa aatattccac caagaatcgg aataaataat tctttcttaa taagtactgc
      301 aataattcca aatgctccgc ccagcgcaag tgatcctgta tcgcccataa aaacctgagc
      361 cggataagta ttaaaccaga gaaaccctaa accggcacca acgagtgctg caataaaaac
      421 agtcaattca ccagatccag gcaaatgaat tatgttcaaa taattagcaa agattgtatt
      481 tccactaaag tatgaaagca atgcgagtgc aatcataact ataacaatta ctccggttgc
      541 taacccatcc aaaccatcag ttaagtttac tgcatttgat gtcgcagtaa ttataaagac
      601 taccaacgga atatagagta ttgaaaaatc cagatttaca ttttttaaaa atggtacagt
      661 tgttaaagtg ctaacaccat gaaattccgg tgaaaaataa attactgatc caacgactaa
      721 tcctattaaa acctgtccca ttaatttata tcgtgcgatt aatccattgg gatatttttt
      781 tattactttc aaataatcat ctaaaaatcc tacagcactt aaccaaagtg taccgacgag
      841 aattaatatt atataggtgc tctttaaatc accccacaac aaaacaggaa ttgtaacaga
      901 cagtacaata attaagcccc ccattgtggg tgtgccgact tttgaccaat gaaacttagg
      961 tccatcagct ttttttgctt cgccgatttg atggacttga agcttcttaa taatctttgg
     1021 cccgagatag agcgaaagaa ataatcccgt tattgcagca agcgctgaac gaaatgttaa
     1081 gaatctaaat atatcaaatc ccggaggatt gaatgtttta tttatgtact caaataaata
     1141 gtaaagcatt tagttttttg ctgattcaat cactgaaaca aattcttcca ttttcattcc
     1201 gcgtgatcct ttaatgagca caacagaatt attaataaca gatgcttgca aatatttttt
     1261 taatgattct ctattactga agtgtttatg aacttttgtt tttccattca atttttgatt
     1321 cataaatttc atcatcttgc caattgagta aacttcatca actttagttt tattaatgaa
     1381 atttgccaga tctaagtgtt ttgatttaga ttcatttcct aattcaaaca tatccccaag
     1441 cacagcaatt tttctttttc gtgatttgat cttattcaag atctctattg acgatttcat
     1501 cgaatcagga tttgcattat aagtatcatt tataacagtg aactttttat agttcttaat
     1561 ttctaatcgc ttgttataag atttaagttt ctttactgct tttaaaattt gagtacttgt
     1621 taatcccaat tctgaagcaa ttgcataagc gacagtaaag tttagtacat ttttttcacc
     1681 gctgattggt aatgctacag ttaaactatt tttcttattt ttaatttcaa tctgaggtct
     1741 tgctactttg tcatagcctt taatcgatac tttaaaatca gctttttcat ttaaagaata
     1801 tgtaatttta ttcttaacag tctttccata ctcttttaag attttatcat tattgtttat
     1861 aaaaactttt ccgccgcgtt taattgtttc atgtaataac gctgattttt ctttaaacac
     1921 acctttgcga tttttcaaat attctaaatg ggaatcacca atattagtta tcaacccaaa
     1981 atcaggactc gcaatcggtg ctgtatatgg aa
//
LOCUS       DASWBY010000343         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_314167, whole genome shotgun sequence.
ACCESSION   DASWBY010000343 DASWBY010000000
VERSION     DASWBY010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314167"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VF870_06395"
     CDS             complement(<1..1194)
                     /locus_tag="VF870_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX9251853.1"
                     /translation="MKEYRFKLILILAAIALSIYLLYPTFQDYQNSKKLTTELEAKRN
                     EIKKLNPDLSKEQVEKLVTLAEDSIKIADPSILEARKKRVKLGLDLQGGMRVVLEVNT
                     GKLLEKIANNPDDVFKKVLAEAQAEALKTDESVVDILGRKFKERNIRLSRYYGTIRQD
                     DAEIMGELKKSAEDAVNRAMEIIRNRVDQYGVSEPSISRQGSRRIIVELPGVAKEEEA
                     KQLLQGTALLEFRLVKDAELTYPIMQRIDDVLAKRNQSGVKDSLGNEVAALDTAKKVT
                     DTTSAADTTKQLSEEEFKAQHPFFTAAVLNPQSPYADAYVSKDDRNKITYWLSLPEVQ
                     KVIPDNVEFVFSAKPITSKDGKQIFIMYLVNKTPELTGGVVTNAQANIDPNTSSPIVN
                     MEMNSE"
     gene            complement(1387..2208)
                     /locus_tag="VF870_06400"
     CDS             complement(1387..2208)
                     /locus_tag="VF870_06400"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX9251854.1"
                     /translation="MVDLSFKYKLLLDYLENESPFKPDLSIILGSGLGDFAEQLQLHK
                     SISTSDIPGYPESTVVGHKGKIHFAEYENKKIILFQGRIHFYEGYNINECILPVFISY
                     KLGVKNLLLTNAAGGINKNFAPGDLMLATSLNGILIKKELTDLIGLTNQSGKNNFINL
                     PDQKFNSLIKSAAKSENILLQEGVYWYTKGPTYETPAEIRFFAKYGADAVGMSTVYEA
                     VYSSYLGLKTAAISCITNFASGISSQKLSHSEVTETADRVKETFARLVKHIIKSL"
     gene            complement(2208..>2563)
                     /locus_tag="VF870_06405"
     CDS             complement(2208..>2563)
                     /locus_tag="VF870_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX9251855.1"
                     /translation="GLVLRFVAFGIGVPWQDSLTFGSLLGTKIVLNEFVAYSDMGNLV
                     QTGKLVNEKSILMATYALCGFANFSSIAIQIGGLGPLAPNRKSDIAALGIKAVIGGVL
                     TTLLTATLAGLFFSF"
BASE COUNT          784 a          465 c          418 g          896 t
ORIGIN      
        1 ttcagaattc atttccatat ttacaattgg cgaagatgta ttaggatcaa tattagcctg
       61 agcattggtt acaacaccac cagtaagttc tggggttttg ttaaccaaat acattataaa
      121 aatctgtttg ccatcctttg aggtaattgg tttagctgaa aatacaaatt caacgttatc
      181 agggattact ttttgtacct caggtaagct taaccagtat gtgatcttgt ttctgtcatc
      241 cttactgaca taagcatcag cataaggact ttgtggattt aataccgctg cagtaaagaa
      301 aggatgctgt gctttaaatt cttcttctga caattgttta gttgtatcag ctgctgaagt
      361 agtatcagtt accttttttg cagtatctaa cgcagcaact tcatttccta gtgaatcttt
      421 aacacctgat tgattcctct ttgcaagaac atcatcaatt ctttgcataa ttggataagt
      481 taactcagcg tcttttacaa gtctaaattc taaaagtgct gtgccctgta aaagctgctt
      541 tgcttcttct tcttttgcaa caccgggaag ttctacaata attcttctgc ttccttgtct
      601 ggaaattgat ggttcagaaa caccgtactg gtcaactctg tttcttataa tttccattgc
      661 acggttaaca gcatcttcgg ctgatttttt taattcaccc atgatttcgg catcatcctg
      721 tctgatggtt ccgtaatagc ggcttaatct tatatttctt tccttaaatt ttcttccaag
      781 aatatctaca acggattcat ctgtttttaa agcttcagct tgagcttctg ccagaacttt
      841 tttgaaaaca tcatcggggt tgttagcaat tttttcaagt aatttaccgg tattaacttc
      901 caataccact ctcataccgc cttgaagatc caaaccgagt ttaactctct tttttcttgc
      961 ttcaagaatt gaaggatcag ctatctttat gctgtcttca gcaagagtca ccagcttttc
     1021 cacctgttct tttgacagat ccggattaag cttttttatc tcgtttcttt tggcttcaag
     1081 ttctgtggtt aattttttac tgttttgata atcctgaaat gtaggatata gcaggtatat
     1141 gcttaaagca atagctgcaa ggattaaaat gagcttaaat ctgtattctt tcatacttta
     1201 ttaaaatttt gttactaaaa gggtctttaa aaaaatagct gtcaaactta cgggtaatgg
     1261 cttagaaagt caataaaaat taagggttta tagttacaaa catactcata aacagtggat
     1321 tttagttgag aaaagaagtt tcaccaaata tttctgcctt cagttttcaa tttatgaatc
     1381 taataattat aaagatttaa ttatgtgctt tactaatctt gcaaaagttt cttttactct
     1441 gtccgcggtt tctgttactt ctgaatgaga tagtttttga gatgatattc cggaggcaaa
     1501 atttgtaatg caggaaattg cggcggtttt cagcccaagg taagatgaat aaactgcttc
     1561 gtaaactgtg gacattccaa ctgcatcggc accatacttt gcaaagaatc tgatctcagc
     1621 aggagtttca taagttggac cttttgtata ccaataaact ccttcctgaa gaagaatgtt
     1681 ttcacttttt gcagcagatt ttataagact attaaatttc tgatcaggca gatttataaa
     1741 attatttttt cccgattgat tggttaatcc aattaaatcc gttagctctt ttttaattaa
     1801 aatgccattt aaagaagttg caagcattag atcaccggga gcaaaatttt tatttattcc
     1861 tcctgcggcg ttggttagta aaagattttt aacaccaagt ttatatgata tgaaaacagg
     1921 aaggatgcat tcattaatat tatatccttc ataaaagtgt attctgccct gaaataaaat
     1981 tatttttttg ttttcatatt cagcaaaatg aattttaccc ttatgcccaa caactgtaga
     2041 ttcgggataa ccaggaatat ctgaagttga aatagattta tgtaattgca attgctcagc
     2101 aaaatcccca agtccactgc caagaattat tgataaatct ggtttgaaag gagattcatt
     2161 ttctaaataa tctaaaagca atttgtattt aaaagataaa tcaaccatca gaatgaaaag
     2221 aataatcctg ctaaagttgc agttaataaa gttgtaagta caccgccgat aacagcttta
     2281 atcccaagcg cggcaatatc tgattttcta tttggtgcaa gtggtccaag tccgcctatc
     2341 tgtattgcaa tggaagaaaa atttgcaaat ccacatagag cataagttgc cataagaatt
     2401 gatttttcgt ttacaagttt accagtttga acaagatttc ccatatctga gtatgcaaca
     2461 aattcattta acacaatttt ggtcccaagt aaacttccaa atgttaaact atcctgccag
     2521 ggcacaccga ttccaaatgc aacaaaccgc agaaccagac caa
//
LOCUS       DASWBY010000344         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_727274, whole genome shotgun sequence.
ACCESSION   DASWBY010000344 DASWBY010000000
VERSION     DASWBY010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727274"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VF870_06410"
     CDS             complement(<1..643)
                     /locus_tag="VF870_06410"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9251856.1"
                     /translation="MKTIKNNFKNIMLLILLVLFALNSNTSAQQVTGLSGWNIFLDPG
                     HSQNENVGIYGYSEAKKNLRVALNLRQMLLDLTDIDTVYICRENDQQLVSLTQRTDYA
                     NSLGAAHYHSIHSDAATMGSPANSTLIMWGQLGIGGPEKIPHGGKKMSNIMVGLLTAG
                     MRTTTRGAMGDRDFYQVAGSTPYLHVNRETNMASELSEAGFHTNPTQNQLNMNA"
     gene            complement(658..1581)
                     /locus_tag="VF870_06415"
     CDS             complement(658..1581)
                     /locus_tag="VF870_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251857.1"
                     /translation="MKKIFLLVFISFTALITAQSNNQLLIEGTPEDAVMNPTYSPDGL
                     KIAYTKSGYQGLWVLDLKAQSTKQITDEAAAGFGYKWSSDSKSILNRVAKYEDMQRMN
                     AVKVFNIETGESNQISDYKTMMPYLPMWADVDSKVYLPLKGKDEVYATGFEKNNLITN
                     DIITFEKNNKITVMNFISNSEYNLEPIKDAQYLNLSLSPNKTKMVFEIMGGNMFVVNT
                     DGTNLIDLGKGNRPKWSFDSKKIIYMITEDDGHEFTSSDIYIINADGTGKVNVTKNET
                     VLEMSPGFSPDGKSVVFENYNEGSIYLMNIE"
     gene            1750..>2043
                     /locus_tag="VF870_06420"
     CDS             1750..>2043
                     /locus_tag="VF870_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251858.1"
                     /translation="MKNTITIFLQQGNPKENQKTIKRFSDSALVKEIILIASDEKFSS
                     FENAKVINCKNFKSSEAIKVIAKYSSTDYTLIVLTEKAVMPGQFCLDRYYQVAI"
BASE COUNT          653 a          362 c          305 g          723 t
ORIGIN      
        1 ttgcattcat atttaattga ttttgtgttg ggttggtgtg aaatcctgct tcacttaatt
       61 cggatgccat attagtttca cggtttacat gtagatatgg agttgaacca gcaacctgat
      121 aaaaatctct atcgcccatt gcaccacgag tggtagttct cattcctgca gttagtaatc
      181 caaccattat attagacatt tttttaccgc catgaggaat tttttccgga cctccaattc
      241 ctaactgtcc ccacattatt aaggtactat tagcagggct acccattgtg gctgcatcgc
      301 tgtgaattga atgataatgt gcagcaccaa gagagtttgc ataatcagtt ctttgtgtta
      361 atgaaaccag ttgctgatca ttttccctgc aaatgtatac tgtatcaatg tctgtcaaat
      421 caagcagcat ttgtctcaaa tttaatgcaa ctctcaaatt cttttttgct tccgagtaac
      481 cataaattcc aacattttca ttttgactgt gtccgggatc taaaaaaata ttccatcctg
      541 aaagtccagt tacctgctgt gcagaagtat ttgagtttaa agcaaataaa accagtaaga
      601 ttaaaagcat tatattttta aaattatttt taattgtttt cattttcatt tactccgtta
      661 ctcaatattc attaaataaa ttgaaccttc attgtagttc tcaaacacaa ctgattttcc
      721 atcgggtgaa aaacccggac tcatttcgag gactgtttca ttttttgtta catttacttt
      781 gcctgtacca tcagcattta ttatataaat atctgaagag gtaaattcat gcccgtcatc
      841 ttcggtaatc atatatataa tttttttact atcaaaagac catttaggtc tgtttccttt
      901 tccaagatca ataagattag ttccatcagt atttacaaca aacatgttac cgcccataat
      961 ttcaaatacc atctttgttt tattaggaga taacgagaga tttaaatatt gggcatcctt
     1021 aattggctca aggttatatt cagaattact tataaaattc attacagtta tcttattatt
     1081 tttttcaaaa gtaattatat catttgtgat tagattattt ttttcaaatc ctgttgcata
     1141 aacctcatct ttacctttta gaggcagata tacttttgaa tctacatcgg cccacatcgg
     1201 caggtacggc atcatagttt tgtaatcgga tatttgattt gattcgcctg tctcaatatt
     1261 aaaaactttt acagcattca ttctctgcat atcttcatac ttagcaacac gatttaaaat
     1321 tgatttggaa tcggatgacc atttatatcc gaatccggct gcagcttcat cagtaatttg
     1381 ctttgttgat tgtgctttta agtctaaaac ccataaaccc tgatatcctg actttgtata
     1441 agcaattttc aaaccgtcag gagaatatgt tggattcatt actgcatcct caggagtccc
     1501 ttcaattaaa agctggttgt tcgattgagc cgtgatgagt gcagtgaatg aaatgaaaac
     1561 aagcaagaat atttttttca tcgtaaaaaa ccttgtattt attaaatgga accagataat
     1621 gtgaatgata tttattattt acaattcact tcaaacaaaa taaaaaaaaa aataacttac
     1681 atacagtcta ctttcttatt tggtgaagtt atatttttat acagtctata aataattatt
     1741 agaactaaaa tgaaaaacac aattacaatt ttccttcagc aaggcaaccc aaaagaaaat
     1801 caaaaaacaa ttaagcgatt cagtgattct gcattggtta aagaaattat tcttattgcg
     1861 tcagatgaaa aattttcatc ttttgaaaat gcaaaagtta ttaactgcaa aaactttaag
     1921 agcagcgaag caataaaagt gattgccaag tattcgtcta ctgattacac cttaatcgtt
     1981 ctgactgaaa aagcagttat gccgggccag ttctgtctgg atagatatta tcaggttgca
     2041 ata
//
LOCUS       DASWBY010000345         4628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_501043, whole genome shotgun sequence.
ACCESSION   DASWBY010000345 DASWBY010000000
VERSION     DASWBY010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501043"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF870_06425"
     CDS             <1..379
                     /locus_tag="VF870_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX9251859.1"
                     /translation="VLKQTKLDTNQIKIIGFGLKKMTVIKKNFSEYEVFLNRRISLAK
                     VIYRKSYWDNFIIKLKKSALDGVNLSYTKSLNTRMVEKFRLNNLKIFIWTVNTPKKVL
                     HLINLAVDGFMSDHAGWIREKVT"
     gene            382..1335
                     /locus_tag="VF870_06430"
     CDS             382..1335
                     /locus_tag="VF870_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251860.1"
                     /translation="MKRESKIIIAIHGLGNKPPKEKLENWWKQAITDGLKFNNYHESN
                     FDFELIYWADILHPDPLDVNEEVKSKQLFTEIYTSVDYSNQKENLSYTNKAKEYLEKY
                     YGKFIVNEVLSLKYPTLTELFVYLHLKDLKTYFSESPVPFNGKEVKAKEAIIDRFLKC
                     LNEHKNKKILLIAHSMGSIIVQDALTEHLPEAQIDTYVTIGSPLGQKYVINKYKLERE
                     QNLKNKLVVPEQIINKWYNLSDVEDQVALNHEQTKLYELNSRKVKIEDQIVKNLFNSA
                     GIKNPHKSYGYLRTPEFSKIINSFLVQKPAGIFAWIRKIFS"
     gene            1478..2599
                     /locus_tag="VF870_06435"
     CDS             1478..2599
                     /locus_tag="VF870_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251861.1"
                     /translation="MDYEIIEIGGTNKLLKKFISFPRYIYQSNQYYVQPLLSEIKEKI
                     NPLYNPFFKYGKIKMFGVIDNNKNLVGRIVAIINPVYDKLYGKEYGFFGMFESINNKE
                     VASLLINQVKSFFNEMNYKRLIGPVNFTTNDESGILIEGFDQSPTFMCAYNEKYYQDL
                     IEGCGLVKAIDMFAYKGNVDHIYPEKFNKILNRILIDPNIKLRTFDKTIKEEDTKIIF
                     NLYNECFKDVWGFVPLSLDEANLMTGKFLPFYDPELIWIAFINSIPAGFILGLPDINK
                     VLKKINGKLFPFGIIRFYLNKNKIDSIRVLTLGVLPQFRGLGLESLLIHRLHERMKAA
                     PYSWGEFSFIMENNFKMRKVLENLGFKAYKRYRIYETNI"
     gene            2610..3929
                     /locus_tag="VF870_06440"
     CDS             2610..3929
                     /locus_tag="VF870_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9251862.1"
                     /translation="MRILLISPTALDFYGNPIKQGRIHLPGLTLPMLAAVTPPEVELK
                     LIMETTQDIPFNEHWDAVGLTGMGSGIVRAWQIASIFKENKVKVIIGGIAASLADPEL
                     ILKHCDALVIGEAEELWALVIEDLQRGTLKRIYKSDHRPDIENLPVPRYDLIRNLKIG
                     LWRPVQATRGCPFTCNFCSVASFSDRNYRKKPIHDVIRDVRAAKKYGTKYIAFIDDNI
                     CVDLDYCHDLFENLIEEKIIWMSQSSLHLTEHPELVKLAYKSGCRILSIGIESLNKSS
                     LEQIDKNWNNPERYASAFKVLRNNGIDVSTEMIIGLDGDDDSVFQNTYNFIVKNKISI
                     PRVHIITPVPGTQLFERMLSDNRITSQDFGRYSGGKVNFIPKNIKAEILQKGYWSLYK
                     ELFSWHSILKRIFINEANLDPYMQAVVWAVNLRYKYHVHHRICPGIV"
     gene            3940..>4628
                     /locus_tag="VF870_06445"
     CDS             3940..>4628
                     /locus_tag="VF870_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX9251863.1"
                     /translation="MKILLIRPPVPKYTIGLKHIMICEPLELEYVAAGLEGHEVKIID
                     LIIEKNFLKRLKKFNPDVIGTSSYINGVNEVIKICRIIKNWNPNCLTVVGGVQASLVP
                     EDFADNSINIIVLGDGTIKFREIVEHYYAGLPFDTIAGLAIVSGKNKIFRTAKSDYMT
                     DPDKLPFPRRDLLLHISHKYYYLFHQPVTLMKTTWGCWYKCNFCFNWKVTCGIPYTRS
                     PESIVKEIETIP"
BASE COUNT         1699 a          678 c          815 g         1436 t
ORIGIN      
        1 agtgttgaag caaactaagc tggataccaa tcaaattaaa ataattggat ttggcttgaa
       61 gaaaatgaca gtaataaaaa agaatttttc cgagtatgaa gttttcttga atagaagaat
      121 cagtttagca aaagtaatct acagaaaatc ctattgggat aattttatta ttaaacttaa
      181 gaaatcagca ttggatggag taaatttatc ctacacaaaa tcattaaata cgagaatggt
      241 tgaaaagttc aggctgaaca atctgaaaat ttttatctgg acagttaaca ctcctaagaa
      301 agtcctgcat ttaattaatt tagctgttga tggttttatg tcagatcacg ccgggtggat
      361 acgagaaaaa gtaacttaac tatgaaaaga gaatcaaaaa ttattattgc catacatgga
      421 cttggaaata aacctccaaa agaaaaacta gaaaactggt ggaagcaggc aataacagac
      481 ggactaaaat tcaataatta tcacgaaagc aattttgatt ttgagcttat ctattgggca
      541 gatattctgc atcctgatcc gctcgatgta aatgaggagg taaaatcgaa gcaactattt
      601 acagagatat acacttcagt tgattattca aatcagaaag agaacttatc ttatactaat
      661 aaggctaaag aatatctgga aaagtattac ggcaaattta tagtcaatga agttttatca
      721 ttaaaatatc caacactaac ggagctgttt gtttatctac atctaaagga tcttaaaact
      781 tatttttcag aatcaccagt acccttcaat ggtaaggaag taaaagcgaa ggaagcaata
      841 attgaccggt ttctgaaatg tttgaatgag cataagaata aaaagatttt gcttatcgca
      901 cattcaatgg gttccataat tgtgcaggac gcattaacgg aacatttacc tgaagcgcag
      961 attgatacgt atgttacaat cggctcacca ttaggacaaa aatacgttat taataaatat
     1021 aaacttgaga gggagcaaaa cttaaaaaat aaattagttg tacctgaaca gatcattaat
     1081 aaatggtaca atttatccga tgttgaagat caggttgctt taaatcatga acagacaaaa
     1141 ttatatgaac taaattcaag aaaagtaaaa attgaagacc agatagttaa aaacctgttt
     1201 aacagtgctg gtataaaaaa tcctcataaa tcttatggat atttaagaac tccagaattt
     1261 tcaaaaataa tcaatagctt cctcgttcaa aaaccagccg gtatatttgc atggataagg
     1321 aaaatattta gttaataaat ctttcactcg atcaaattta attgaatcta agatttatta
     1381 gttgctaact gcataccaat tctcccctat tttttacaat ttcatgacaa ctcaaaatta
     1441 aatccagctt aaattacatt aagcaattga aattgaaatg gattatgaaa tcattgaaat
     1501 aggtggtacg aataaattat taaaaaaatt tatttccttc ccacgatata tttatcaatc
     1561 taatcaatat tatgtccagc cattactgtc cgaaataaaa gaaaaaatta acccattata
     1621 caaccccttc tttaaatacg gcaaaataaa aatgttcggt gttattgata ataataaaaa
     1681 tttagtggga cgtatagttg caataattaa tccggtttac gacaaacttt atggtaaaga
     1741 gtatggattc tttggaatgt ttgagtctat aaataataaa gaagttgcat cattacttat
     1801 taatcaggtt aaatcattct ttaatgagat gaattataaa cgactgatcg ggcctgtgaa
     1861 ctttacaacc aatgatgaaa gcggaatttt aatcgaaggc tttgatcaaa gtcctacctt
     1921 tatgtgtgct tataatgaaa aatattatca ggatttaatt gaaggttgcg gtcttgtaaa
     1981 agcaatagat atgtttgctt ataaaggcaa tgttgatcat atatacccgg aaaaatttaa
     2041 taaaatcctt aaccggatct taatcgatcc aaatataaag ttacgtacat ttgataagac
     2101 aataaaagaa gaagacacaa aaataatttt taatttatat aatgaatgct ttaaggatgt
     2161 ttggggattt gtcccccttt cgcttgatga agctaatctg atgactggaa aatttctacc
     2221 attttatgat ccagaattaa tttggatcgc atttatcaat agcataccag ccggatttat
     2281 tttaggatta ccggacatta ataaagtact gaaaaaaata aatggtaaat tatttccatt
     2341 cggtattatc agattttatt taaacaaaaa taaaattgat tcaattagag ttttgactct
     2401 gggcgtgtta cctcaattca gaggattagg attagaatct ttattaatcc atagactaca
     2461 tgaaaggatg aaagcagctc catatagttg gggtgaattt tctttcatta tggaaaataa
     2521 ttttaagatg cgaaaagttt tggagaatct cggtttcaaa gcttataagc gttacaggat
     2581 ttatgaaaca aatatttaag caatcaaaaa tgcgaatact acttatttca cctacggcat
     2641 tggatttcta tggtaacccc ataaagcaag gaagaataca tttacctgga ttaactcttc
     2701 caatgctggc agcggtcact cctcctgaag ttgaattaaa acttatcatg gaaactaccc
     2761 aggatattcc attcaatgaa cattgggatg ccgttgggtt aacaggtatg ggatcaggaa
     2821 ttgttagggc ctggcagatt gcatccatat ttaaagaaaa taaagttaaa gtaataattg
     2881 gggggattgc cgccagttta gcagatccag aattaattct aaaacattgt gacgcactgg
     2941 ttataggtga agcagaagaa ttatgggctt tggtaattga ggacctgcag cggggaacat
     3001 taaagcgaat atataaatct gatcataggc ctgatatcga gaatttgcca gttccacgat
     3061 acgacttaat caggaattta aaaatcgggc tttggcgccc ggttcaagct accagaggat
     3121 gtccgtttac ctgcaatttt tgttcagtag ctagtttcag tgatagaaat tatagaaaga
     3181 aacctataca tgatgtaatc agagatgtca gagctgcaaa aaaatacggg actaaatata
     3241 ttgcctttat agacgataat atctgtgtcg atttagacta ttgtcatgat ctttttgaaa
     3301 atttaatcga agagaaaatt atctggatga gtcagtcaag tctgcattta acagaacacc
     3361 cggaacttgt taaacttgct tacaaaagtg gttgtcgaat tctttcgata ggcatagaat
     3421 cattaaacaa atcaagtctc gagcagattg ataaaaactg gaataatcct gaaagatatg
     3481 cttctgcctt caaagtctta agaaataatg gaatagatgt ttcaactgaa atgattattg
     3541 gattagatgg tgatgatgat tcagtatttc aaaacacata taattttatc gttaaaaata
     3601 agatttcaat acctagagtg catattataa ctcccgttcc cggcactcaa ttattcgaaa
     3661 gaatgctttc tgataacaga attacctctc aggattttgg acgttatagc ggcggtaagg
     3721 ttaatttcat accaaaaaat attaaggctg aaatactaca gaaaggatac tggagtttat
     3781 ataaagaatt atttagctgg cattcgatcc tgaaaaggat ttttattaat gaagcaaatc
     3841 ttgatcctta tatgcaggca gttgtttggg ctgtaaattt aagatataag taccatgtac
     3901 atcatagaat atgtccagga atagtttaga ggctgtttta tgaaaatatt actaataaga
     3961 ccacctgtcc ctaaatatac aatcggttta aagcacatta tgatttgtga acctcttgaa
     4021 ttggaatatg ttgcggcggg attagaaggt cacgaagtca agataattga tcttatcatt
     4081 gaaaaaaatt ttcttaaacg attaaaaaaa tttaatcctg atgtaatcgg aactagctct
     4141 tatataaatg gcgtaaatga agtaataaag atttgcagga taatcaagaa ttggaatcca
     4201 aattgtctta ctgtcgttgg tggagttcag gcatcacttg tgccagagga ttttgctgac
     4261 aattctataa atattattgt acttggtgat ggcacaatta agtttagaga aatagtagaa
     4321 cattattatg caggattacc atttgataca atagcgggtt tagcaattgt aagcggaaaa
     4381 aataaaattt ttaggacggc taaatcggat tatatgacag atcctgataa attacctttt
     4441 ccaagaagag atttactttt acacatttca cataaatatt attatctctt tcatcagcct
     4501 gtaacattaa tgaagacaac ctggggctgc tggtataaat gtaatttttg cttcaactgg
     4561 aaagtaactt gcggaattcc atatacacgc tcccccgaat caatagtaaa agaaattgaa
     4621 accatacc
//
LOCUS       DASWBY010000346         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_49780, whole genome shotgun sequence.
ACCESSION   DASWBY010000346 DASWBY010000000
VERSION     DASWBY010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49780"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1422
                     /locus_tag="VF870_06450"
     CDS             280..1422
                     /locus_tag="VF870_06450"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HEX9251864.1"
                     /translation="MQKSLILSAILFIIIIGCSSTPEIVPDPKQVTFLTNGIWLGMLP
                     CADCEGIDYQLNLKNDYTFKQKSVYKGKSENIFINEGTWGFVSDSVIAVSGNEDYKMF
                     IILGNDLVMLDKDGNRIESNFEERYHLSKDDGSVKETKEVKPVPHVVEMNLSYYQEKF
                     LNGIDFFARGNEPNWTLEIDLEKSMAFATMYDIKLNTPAVEGIKAQEADVTLYSAKTD
                     SGELIVTIIKDKCQDNMSGEYFPYKVRIEAKTSNDSSYKTFEGCGKYLYDIRLHDIWV
                     MKEMTESDLKKEKLMKGMPQFEFNLTDMRFAGHAGCNSISGPIDVVDNKITFGNLMGT
                     LMACPNMKVEKAVVNALNQKTVTYSIDKLKLTLVSGKTKMVFQKVD"
     gene            complement(1488..1754)
                     /locus_tag="VF870_06455"
     CDS             complement(1488..1754)
                     /locus_tag="VF870_06455"
                     /inference="COORDINATES: protein motif:HMM:NF016262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEX9251865.1"
                     /translation="MILDSNNKKPEINYPCDWHYKVIGTDADEMIKAIELAAEGLDYK
                     ISPSNISSKGNYFSLNLKVFVTSEVLRDIIFAKLKGNEFIKMVL"
     gene            complement(1760..2299)
                     /locus_tag="VF870_06460"
     CDS             complement(1760..2299)
                     /locus_tag="VF870_06460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF015020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9251866.1"
                     /translation="MDISQKILLILDLDETLIYSTKSRLYYTEDFMVDEYCVYKRPGL
                     DKFLDFISPNYDVAIWTSSTEDYAQEVVKNIFPQNYPLKFIYGRTKCTITFLWDQNEY
                     VYAKNLSKLKKKNYSLEKILIVDDSPEKLKRNYGNHIRVEPFVGSKEDIELFQLIDYL
                     DKIKSIKNVRNIEKRTWRL"
     gene            complement(2316..>2538)
                     /locus_tag="VF870_06465"
     CDS             complement(2316..>2538)
                     /locus_tag="VF870_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9251867.1"
                     /translation="SILFLGLLCSGVSYVIWAYALREMESAKVGAFLYFEPFVTVVTA
                     WILLKEDITLLMVLSGMIITFGVFLVNKD"
BASE COUNT          914 a          368 c          393 g          863 t
ORIGIN      
        1 tttttctttt aaagattttt agacattaag atttataata ttgaataagt aaatcaaaat
       61 cttaaagtaa aaaatcacat aaaattttat caacaagtaa aagttttcta ccataatcac
      121 cgaagctctt taatcaaaga gaaactttat tcgtttattt agcaaattta tcattaccaa
      181 atagattgat gacggtataa tttttgaaat ttataaatta tatgaaatct ccattggtaa
      241 tcaacaaaat gataattaat tacagctgag gaatttatta tgcaaaaaag tttaatatta
      301 tctgcaattt tatttataat tataattgga tgttcttcca ccccggagat tgtacccgat
      361 cccaaacaag ttactttttt aactaacgga atttggctgg gtatgctgcc atgcgctgat
      421 tgcgaaggaa ttgattatca gcttaatttg aaaaacgatt atacttttaa acaaaaatca
      481 gtttacaaag gtaaatctga aaatattttt atcaatgaag gaacatgggg ttttgtttcg
      541 gattcagtaa ttgcagtctc aggcaatgaa gattacaaaa tgtttattat tcttggaaat
      601 gatttggtta tgttggataa ggatggaaac agaattgaat caaactttga agaacgatat
      661 cacttaagca aagatgacgg ctctgtaaaa gagaccaaag aggttaagcc agttccgcat
      721 gttgttgaaa tgaatttaag ttattatcag gaaaaatttt taaatggaat tgattttttt
      781 gccagaggta atgaacctaa ctggactctg gaaattgatt tagaaaagtc gatggcattt
      841 gcgactatgt atgatataaa attaaatact cccgctgtgg aaggcattaa agcacaagaa
      901 gcagatgtta cattatatag tgcaaaaaca gatagtgggg aactgatcgt tacaattatt
      961 aaagataaat gtcaggataa tatgtcagga gaatattttc cttacaaagt tcggattgag
     1021 gctaaaacgt ctaatgattc aagctacaaa acatttgaag gatgtggaaa gtatttatat
     1081 gatattcgtt tacatgatat atgggttatg aaagagatga cagagtctga tcttaaaaaa
     1141 gaaaaactaa tgaaaggaat gccgcaattt gaatttaatc tgaccgatat gcgttttgcc
     1201 ggacatgcag gctgcaacag catttcggga ccaatcgatg ttgtcgacaa taaaattacc
     1261 tttggaaatt taatgggtac tctaatggct tgtccaaata tgaaagttga aaaagcagtt
     1321 gtaaatgcac ttaatcaaaa aacagttaca tatagtattg ataaattaaa actgactctg
     1381 gtaagtggaa aaactaaaat ggttttccag aaggtggatt aaataattta aagattaaaa
     1441 ggagtttaaa aacatagtca atctggtttt ggttttataa tagaacatta caataccatt
     1501 ttaataaact cattgccttt aagctttgca aatattatat cgcgaagaac ttctgatgtt
     1561 acaaatacct ttaagtttaa actaaaataa tttcctttag aactgatatt tgaaggagaa
     1621 attttatagt ctaatccttc agcagcaagc tctattgcct ttatcatttc atccgcatcc
     1681 gtcccgatta ccttataatg ccaatcgcag ggataattta tctcaggttt tttattattt
     1741 gagtctagaa tcatttactt tataatctcc aagtcctctt ttcaatattt ctaacatttt
     1801 taattgattt gatcttatcc aaataatcta ttaattgaaa tagttcaata tcctcttttg
     1861 accccacaaa tggttccact cttatatggt ttccatagtt tctttttaat ttttcaggac
     1921 tatcatcaac aataagaatc ttttccaagg aataattttt ctttttaagt tttgaaagat
     1981 tttttgcgta aacgtattca ttctgatccc agagaaatgt tatggtacat tttgttcttc
     2041 cataaataaa tttaagagga taattttgag gaaaaatatt cttaacaact tcttgcgcat
     2101 aatcctctgt cgatgaagtc caaattgcaa catcataatt tggtgaaata aaatctaaaa
     2161 atttatctaa gcccggtctt ttataaacac aatattcatc caccataaaa tcttccgtgt
     2221 aatataatcg agattttgtg gaataaatta atgtttcatc taaatcaagt ataagtaaaa
     2281 ttttttgcga gatatccatt ttatattata tggttttaat ctttgttaac cagaaatact
     2341 ccgaatgtaa ttatcattcc gcttaaaacc attaataagg taatatcttc tttaagcaaa
     2401 atccaggcgg taactacagt tacaaaaggt tcaaaataaa gaaaggctcc gacctttgcc
     2461 gattccattt cacgcaaagc atacgcccat attacatatg atacaccact gcagagtaag
     2521 cctaaaaata atattgaa
//
LOCUS       DASWBY010000347         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_538093, whole genome shotgun sequence.
ACCESSION   DASWBY010000347 DASWBY010000000
VERSION     DASWBY010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538093"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VF870_06470"
     CDS             <1..661
                     /locus_tag="VF870_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9251868.1"
                     /translation="AAFLVEPIQGEAGVFVPDDGYLKKAQELCKAKNVLLICDEVQTG
                     LARTGKMLASDYEAVKPDILILGKALSGGVMPVSAALANDEVMLAIKPGQHGSTFGGN
                     PLACKVAIASLEVLIEEKLAENAFAMGAYFRSEVQKMVNEFENLTLVRGKGLLNAIVI
                     KPAKSGKTAWDVCVALKENGMLAKPTHGDIIRFAPPLVITKDEIDFAISIIRKVLKDF
                     S"
     gene            complement(767..>2428)
                     /locus_tag="VF870_06475"
     CDS             complement(767..>2428)
                     /locus_tag="VF870_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9251869.1"
                     /translation="RDVTSEVLAQERRNRAELELKTEKEKSDALAREAMKLSTAKSRF
                     LANMSHEIRTPMNGIIGFLSLIENESYQNQDELNQFVRNAKMSAESLLDTINAVLDLS
                     KIEAGKIELENLNFNLKKIVTQAISIVSPKAKEKSLNISYQLPEDKNLNFIGDPTRIR
                     QIYLNLLSNAVKFTKEGEIKIVVKTSEAQKSKIRISTSIYDSGIGIPADKIKELFKPF
                     SQITGIEGSSLGGTGLGLVICKEFANLMEGDISVTSIKGEGSRFDFHIVVQEQVTDID
                     SKQTEDKNTLSEVEELQFTDADIKNHIPKRKGFKILLAEDNLINQKVAIKTLSSAGYD
                     VDAVMNGEQAVRAHTQNHYDLILMDVQMPDVDGFAATKMIRSLPEPKNKIPIIAITAH
                     ALIGDRERCIEAGMDEYVPKPMVAREIIRLIDHFLKINYSDDKKMKDEVYDLRVFDFE
                     RLKQISLGDVVFEKDLLGDYLVDAEQKLQLLWESHSSGEVQKIADLAHTLKGSSYSVG
                     AKLVGDESLGVELSAKNNDLGNVEERLTKLASSIRETREILKNKI"
BASE COUNT          721 a          464 c          428 g          815 t
ORIGIN      
        1 tgcagcattt ttagtcgaac cgattcaggg agaagctggt gtttttgttc ctgatgacgg
       61 ttacttaaag aaagcacaag aactttgcaa agctaaaaat gttcttctca tctgtgatga
      121 ggtacaaaca ggtttggcaa gaacaggtaa aatgcttgca tcagattatg aagcagtaaa
      181 acctgatatt ttaatccttg gtaaagcgtt atcaggcgga gtaatgcctg tatctgcagc
      241 tttagccaat gatgaagtta tgctcgcgat aaaacctgga cagcacgggt caacttttgg
      301 cggcaatcca ttagcttgta aagttgctat cgcatcgttg gaagttttga ttgaagaaaa
      361 acttgcagaa aatgcttttg caatgggtgc ttacttcaga tcagaagttc agaaaatggt
      421 aaatgaattt gaaaatctta cattagtgcg cggtaaaggg ttgttgaatg caattgtaat
      481 caaaccggca aaaagcggaa aaactgcctg ggatgtttgc gtggcattaa aagaaaacgg
      541 aatgctagca aaaccaacgc acggcgatat tatacgattt gcaccgccgc ttgtaataac
      601 aaaagatgaa attgattttg caataagtat tataagaaaa gttttgaaag attttagtta
      661 aataagaaat gtcgtcactt cgccccgatt cctaacgagg cgaagtgata ttttactaaa
      721 tcgatgagtg gattgcttcg tcgtggactc ctcgcaatga ctgtttttaa attttattct
      781 taagaatttc tcgtgtttct ctaattgatg aggcaagttt tgttaatctt tcctcaacat
      841 ttccaagatc attattcttt gcagaaagtt ccaccccgag tgactcatca ccaactaatt
      901 tagcaccaac agagtagctt gatcctttga gtgtgtgcgc taaatcggca attttttgta
      961 cttcacctga tgaatgagat tcccaaagca gttgaagttt ctgttccgca tcaaccagat
     1021 aatcaccaag taaatccttc tcaaaaacca catcaccaag actgatttgt tttaatcttt
     1081 caaaatcaaa aactctcaga tcgtaaactt catctttcat ttttttatca tccgaataat
     1141 taatcttcaa aaaatgatca atcagtctga taatttcacg cgccaccata ggtttgggaa
     1201 catattcatc cataccggct tcaatgcatc tttcacgatc accaataagc gcatgtgctg
     1261 taattgcaat aatcggaatt ttgtttttgg gttccggtaa tgatctgatc attttagttg
     1321 ctgcaaaacc atcaacatcc ggcatctgca catccatcaa aatcaaatcg tagtgattct
     1381 gggtatgcgc acgaactgcc tgttcaccat tcataactgc atccacgtca taacccgcag
     1441 agctaagagt tttaattgca actttttgat ttatcagatt atcttctgca agcagaattt
     1501 taaatccttt tcttttagga atatgatttt taatatccgc atcggtaaac tgaagttctt
     1561 cgacttcaga taaagtattt ttgtcttcag tttgttttga atcaatatct gttacttgtt
     1621 cctgcacaac gatatgaaaa tcaaatctgc ttccctctcc ctttatactg gttactgaga
     1681 tgtcgccttc cataaggttc gcaaactctt tacaaataac cagacctaat ccggtaccgc
     1741 ccaaactgct tccttctatt ccggtaatct gtgagaaagg tttaaataat tccttaattt
     1801 tatcagcggg aattccgata cctgaatcat aaatagaggt gctgattctt attttacttt
     1861 tttgagcctc actggttttt acaacaattt taatttcacc ctcttttgta aacttgacgg
     1921 cattacttaa aaggttaaga taaatttgtc ttattcttgt agggtcacca ataaaattaa
     1981 gatttttatc ttcgggtaat tgatatgaaa tatttaatga tttttctttt gctttgggac
     2041 ttacgattga aattgcctga gttacaattt ttttaagatt gaagttaaga ttttcaagct
     2101 caatttttcc tgcttcaatt tttgataaat ccagaacagc atttattgta tccagcaaag
     2161 actccgcaga cattttagcg ttgcgaacaa actgatttaa ttcatcctga ttttgataag
     2221 attcattttc aatcaaggat aagaatccaa ttataccatt cattggtgta cggatttcgt
     2281 gactcatatt agcaagaaat ctgcttttgg cggtacttaa cttcatagct tctctggcga
     2341 gagcatcgga tttttctttt tcggttttaa gttcaagctc tgctctattc cgccgttcct
     2401 gtgctaaaac ttctgaagta acgtcacg
//
LOCUS       DASWBY010000348         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_238906, whole genome shotgun sequence.
ACCESSION   DASWBY010000348 DASWBY010000000
VERSION     DASWBY010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238906"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /locus_tag="VF870_06480"
     CDS             <1..1732
                     /locus_tag="VF870_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEX9251870.1"
                     /translation="LKLEYQYTGDYLRLYPFNFIKNLGFTGQPNETFLYGVGNDAVSY
                     LLDGVSYNDRQSNSLNLNLIQSEDVDSIEMIPLPRGFLYGAYNNPVSVNFITKDFIPI
                     LPYSRIRFYQGTNRDMMFDGIFNAVVTKKLIASFDITNRIYDGTYANTDYSIWQGKIK
                     LRYLLSNAVNIIASYSYNDYNAGYSGGVNVDSIKSLGENVDNVLYDFRAAPMVYPNGE
                     LKTLTHLPRLRFLIKPTEWMKSDASLFFMFNRYEKNTSTSDYSESKTYGLNIRNDFDY
                     NVFKFQLNVDYEKRDIYSSRTFYNSVVPAQSNTLLSQFNADLFSVSGILSANLDDDTF
                     IPSVFYKTSSYKKEYSQPADFNSSNNGQGVGVDLLFKIKQSFGLYLGTSILKSYNSSK
                     YEYALIELGMKYQDEFVFADLKYFYNEYDYGFYTDTGLDILNYIRFGYVNGLGLNLKF
                     NYWKLLLESNSSYYFAIDNDKLIGVPEFQTQTGLYFKSILFNSNLDLKTGFVFYYTGT
                     NNVFTNEHGVIEVSSSYKLDFTLAGEIQKVAIVYFLWQNLLQNNYYITPYYPMPTSNI
                     RFGFAWELFN"
     gene            complement(1838..>2023)
                     /locus_tag="VF870_06485"
     CDS             complement(1838..>2023)
                     /locus_tag="VF870_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9251871.1"
                     /translation="LDERLDRKSFFRASRKHIINLKWVENIEPWLNGGLLAKLKGGHK
                     IEVSRRQAVKFKEMLSL"
BASE COUNT          644 a          316 c          346 g          717 t
ORIGIN      
        1 gcttaaactt gaatatcaat atacgggcga ttatttaagg ctatacccgt ttaactttat
       61 taaaaactta ggttttacag gacagccaaa cgaaactttc ctttacggtg ttgggaatga
      121 tgcggttagt tatcttttgg atggagtttc atataatgat agacagtcca attctttaaa
      181 tcttaatctt atacaaagtg aagatgttga ttcgatagag atgattcctt tgcctcgtgg
      241 atttctttac ggcgcatata acaatcctgt tagtgtaaat tttatcacaa aagattttat
      301 tccaattctg ccttattcac gaatcaggtt ttatcagggt acaaacagag atatgatgtt
      361 tgatggaatt tttaatgcag ttgtaacaaa aaaacttatt gcttcttttg atattacaaa
      421 cagaatttat gacggaactt atgctaacac agattattcc atctggcagg gaaaaatcaa
      481 attaagatat ttgctttcta atgccgtaaa cattatcgca tcatacagct ataatgatta
      541 taatgccgga tacagcgggg gagttaatgt tgacagtatt aaatcacttg gagaaaatgt
      601 tgataatgtt ttgtatgatt tcagagctgc tcctatggtt tatccgaatg gcgaattaaa
      661 aactttaact catttaccaa gattacgttt tttaattaaa ccaacggaat ggatgaaatc
      721 ggatgcaagt ttatttttta tgtttaatcg atacgaaaaa aacacatcaa caagtgatta
      781 ctctgaaagc aaaacttatg gcttaaatat ccgcaacgat tttgattaca atgtatttaa
      841 atttcagtta aatgttgatt atgaaaaaag agatatttat tcgagcagaa cattttacaa
      901 ttccgtagtt ccggcgcaat cgaatacatt gctgagtcaa tttaatgctg atttattttc
      961 ggtatccggc attctttctg ctaatcttga tgatgacact ttcattccgt cggtatttta
     1021 taaaacatct tcatataaaa aggaatattc acaaccagca gattttaatt ccagtaataa
     1081 cggtcaggga gtcggcgttg atttgttatt caaaattaaa caaagcttcg ggctttattt
     1141 gggaacatca attctaaagt catataattc aagtaagtat gaatatgctc taattgaact
     1201 tggcatgaag tatcaggatg agtttgtatt tgctgatcta aaatattttt ataatgaata
     1261 cgattatggt ttttataccg ataccggttt agatatcctt aattatatta gattcggcta
     1321 cgtgaacggt ttgggactga atctaaaatt caattactgg aaattgctgc tggaatcaaa
     1381 ctcatcatat tactttgcta tcgataatga taaattgatt ggtgttcctg agtttcaaac
     1441 acaaaccgga ctttatttca aaagcatatt atttaatagt aatcttgatt taaaaacggg
     1501 atttgttttt tattacaccg gtacaaataa tgtttttact aatgaacacg gtgttattga
     1561 agtttcatct tcttacaaat tagattttac actagcgggg gaaattcaaa aagtggcaat
     1621 tgtatatttc ttgtggcaga atcttttaca aaataattat tacataacgc catattatcc
     1681 tatgccgact agtaatataa ggtttggctt tgcgtgggaa ttatttaatt agagtttttt
     1741 gtcattgcaa atcccgactt gtcgggatga agcaatctat tgatttgtta gattgcttcg
     1801 ctcccaaagc ctcgctcgca atgacactta aaaagaatta cagactcaac atctctttaa
     1861 atttaaccgc ttgtcttctt gagacttcaa ttttatgtcc gcctttaagt tttgctagca
     1921 atccaccgtt aagccagggt tcaatattct caacccattt tagattgatg atgtgttttc
     1981 tgcttgcacg gaaaaatgat ttgcggtcta atctttcatc aag
//
LOCUS       DASWBY010000349         3724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_538283, whole genome shotgun sequence.
ACCESSION   DASWBY010000349 DASWBY010000000
VERSION     DASWBY010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538283"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1766
                     /locus_tag="VF870_06490"
     CDS             168..1766
                     /locus_tag="VF870_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX9251872.1"
                     /translation="MIQNLSNIDLIIILSYILIVLFIGFYFRKKNKNSVEYFLAGRNI
                     GWIAIGASLFATNISSEHFIGLAGTGSKSGLAVGHFEWLACLILLLLGWVFTPFYLKS
                     GVFTMPEFVEKRYNSASRYYLSLISIIGYVLTKISIALYAGGILLNAVVGWDMVTAAV
                     VIVLITGIYTVMGGLSAVIYTDLVQTIILIGGSIALTLIGLDKAGGWNNLVAKTPIDF
                     WSMFKPLSDPDFPWTGIVFGAPIIGIWYWCTDQYIVQRVLSAKNLSNAQSGTILAGFL
                     KILPVFILILPGIIAFYISDGTVSGDKAYAWLITNLLPSGLKGLVVAGLLAALMSSLS
                     AMFNSTSTLLTIDIYKKIKPDADEKSTVAFGRIATGAMVVLGLLWIPFIGILSDDRMY
                     VYLQSVQAYISPPIAAIFIFGIFWKRVNGKGALASLFTGLVLGAFRLVIEIQNKIEPL
                     SNSFLQNVASINFLHFAIFLFIISSAVLISVSLVTAKPSADKLEGMIYTRNVFVQKDK
                     WRTVNISFSVILVIVLITLWWIFR"
     gene            complement(1851..1928)
                     /locus_tag="VF870_06495"
     tRNA            complement(1851..1928)
                     /locus_tag="VF870_06495"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1891..1893),aa:Val,seq:tac)
     gene            complement(2323..3504)
                     /locus_tag="VF870_06500"
     CDS             complement(2323..3504)
                     /locus_tag="VF870_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9251873.1"
                     /translation="MIPKRILPAIVFAQFAGTSLWFAGNAIITDLRIPMDVGIEDVGI
                     ATSAVQLGFITGTMLFALFSIADRYSPRKLFLVCSILGAISNLLIYFIAYNLFSLLVI
                     RFITGFFLTGIYPIGMKIASGWYKKDLGNAIGLLVGALVLGTAFPHLIKSFGGSVPWE
                     KVIFIISVFSIVGGITMYVFVPDGPYISSGTKFNPNAILTIFKFKALRSSAFGYFGHM
                     WELYTFWALIPVILISYLKLNSAELNIPLWSFIIIACGTFGCVMGGVISKRIGSAKVA
                     LIQLLLSGICCLVSPLMFSTSIPLFLSFLILWGIVVAGDSPQYSAIIALSAPKELVGS
                     GLTLVNSIGFAITILSLWFVYQFLGIIDVSYALMILGVGPIFGLISMKNLVFSKLKIL
                     T"
BASE COUNT         1225 a          655 c          641 g         1203 t
ORIGIN      
        1 tgcgcttgca agcggtgttt atttttatca gctaaaatcc ggcgattttt ttcaatcgaa
       61 aaaaatgaca gttcttaaat aaaataatct aaaactgatt ccctgtttct gaggatatat
      121 caattatcag tatttcttat ctaccctaaa ttaaggtggg agttattatg attcaaaatc
      181 ttagtaatat cgatcttatt ataatcctat catatatttt aattgttctc tttattggat
      241 tttacttccg gaaaaaaaat aaaaacagtg ttgagtattt tcttgccgga agaaatatag
      301 gatggattgc aataggtgct tctttatttg caacaaacat ttccagcgaa cacttcatcg
      361 gacttgcagg aacaggctca aaatcaggtt tagccgttgg acattttgaa tggcttgctt
      421 gtttaattct gttacttctt ggctgggtat tcactccatt ctatttgaaa tcaggtgtat
      481 ttacaatgcc ggagtttgtt gaaaaaaggt ataactcagc atcgcgttac taccttagtc
      541 ttatttcaat cattggctat gtacttacaa aaatatcaat tgcactttat gcgggcggaa
      601 ttttgctaaa tgctgttgtt ggatgggata tggttactgc tgcggtcgtg attgtcttga
      661 ttacaggcat ctacacagtt atgggcgggc tctcagcagt tatttatacc gatctggttc
      721 aaacaatcat tttaattgga ggctcaattg ctcttacact tattggattg gataaagcag
      781 gtggatggaa taatcttgtt gctaaaacac caattgattt ctggagtatg tttaaacctt
      841 taagtgatcc tgattttcca tggactggaa ttgtcttcgg cgctcctatt attggaatat
      901 ggtattggtg taccgatcaa tatattgttc aacgtgtact aagtgctaaa aatttatcta
      961 atgctcaaag cggaactata cttgcggggt ttttaaaaat tcttcctgtt ttcattctta
     1021 ttcttcccgg tataattgca ttctatattt ccgatggcac tgttagcggg gataaagctt
     1081 atgcatggct tataacaaac ctgcttccat ccggattaaa agggctagta gttgccggat
     1141 tgttagcggc tttaatgtca tctctgagtg caatgttcaa ttcaacatca actcttctta
     1201 caattgatat ttataaaaaa ataaaacctg atgcggatga aaaatccaca gttgcttttg
     1261 gaagaattgc aacaggagca atggttgtat taggattatt atggatcccg tttattggaa
     1321 tattaagcga cgacagaatg tatgtttatt tgcaaagtgt tcaagcatat atatctcccc
     1381 cgattgctgc aatatttata ttcggtattt tctggaaacg agtaaatgga aagggagctc
     1441 tggcctcatt atttaccgga ttagttttag gcgcctttag attagtaata gaaattcaaa
     1501 ataaaattga acctctttca aattcatttt tacagaatgt tgcatcaatt aattttcttc
     1561 actttgcaat atttttgttc attatttcat ccgcagtttt aatatctgta agtcttgtta
     1621 cagcaaaacc ctctgcggat aaacttgaag gaatgattta cacaagaaat gtttttgtcc
     1681 aaaaagataa atggagaact gtgaacatca gtttttctgt tatactggta attgtgttaa
     1741 taactctttg gtggatattt aggtagaatt ctcttttgca aggacatata atcctgagct
     1801 tcttcaaaga ctttaaaatt aaaaaatccc tcgaatgagg gattttttat tggtgggcgc
     1861 tcagggattc gaaccccgga ccctctgctt gtaaggcaga tgctctaaac caactgagct
     1921 aagcgcccaa attgtagatc aaaatttggt ggacaaatat gcggtttttc taaaataaaa
     1981 ccaaacttaa attcttatat tttttaatat aatcgcaact tattcagaac ctttacagaa
     2041 ttactctctt caagatcaag tatttaaatg acatgtctaa tctcatttcc attattatgc
     2101 ttcaccagat tttatttgaa aagtaataaa gtgtactcaa aaaactaatc actttttttg
     2161 atgctaaatt caattcttta aagagcactt gcatacgaaa aagcccaaac ataattgaag
     2221 gaattctact tcccaaaaac ggacagggct tttaaaagtt ataaataatt aatttcttat
     2281 caggtgcttt atatttatca atggccttat tataaatgta aattaggtca aaatttttaa
     2341 tttactgaaa actaaatttt tcatagaaat taatccaaag ataggtccca ctcccaatat
     2401 catcagagcg tatgaaacat ctattatccc taaaaattgg taaacaaacc aaagacttaa
     2461 aatagtgatc gcaaatccaa tactgtttac caaagttaac cctgaaccca ccaattcctt
     2521 tggagcagaa agagcaatga tagcagagta ctgcggtgaa tcacccgcta cgacaattcc
     2581 ccaaagaata aggaatgata aaaataaagg gattgatgtg gaaaacatta gcggcgaaac
     2641 cagacagcaa ataccagaaa gtaaaagttg aataagtgct acctttgcgc tgcctattct
     2701 ttttgaaatt actccaccca taacacaacc aaaagtgccg caagctatta taatgaatga
     2761 ccataaaggg atgttaagtt cggccgaatt tagttttaag taggaaataa gaattaccgg
     2821 tattaaagcc cagaacgtgt acaattccca catatgtcca aagtatccaa atgcagagga
     2881 tctgagtgct ttgaatttga atattgtcag gattgcattc gggttgaact ttgtcccaga
     2941 agaaatatat gggccatcag gcacaaatac atacattgtt atccctccaa caattgaaaa
     3001 aacagaaata ataaaaataa ccttctccca tggaacagaa ccgccaaagc tttttattaa
     3061 atgcggaaaa gcagtcccaa gtaccagtgc acccacaagc aaaccgatag catttcctaa
     3121 atctttttta taccagccag aagctatttt cattccgatg ggatatatcc ctgtcaaaaa
     3181 aaaaccggta ataaatctaa taacgagaag tgagaacaaa ttataagcaa taaaatatat
     3241 tagcaagttt gaaatggccc cgaggattga acataccaga aataactttc tgggtgaata
     3301 tctgtctgcg atggagaaca acgcgaataa cattgttcct gttataaatc ctaactgaac
     3361 cgcagaagtg gcaattccga catcttctat cccaacatcc attggaattc ttaaatctgt
     3421 aattatcgca ttgccagcga accacaatga tgtaccggca aattgagcaa atacaatagc
     3481 aggtagaatt cttttcggta tcatcttggt taataaaatt aattagtgtg tacaaaaaaa
     3541 atacactaaa taaattgccc ctgcctaaaa tctaaagcag gggctaagca aaaaatttat
     3601 ttatccttct tacacagcag gttttatatt ctgattatat tttaacaggt tatcaggatc
     3661 gtactttttc ttgatttgtg taagacgatc gtaatttgaa ccatatgctt ttgcaacgcg
     3721 atct
//
LOCUS       DASWBY010000350         4650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_16150, whole genome shotgun sequence.
ACCESSION   DASWBY010000350 DASWBY010000000
VERSION     DASWBY010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16150"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..936)
                     /locus_tag="VF870_06505"
     CDS             complement(271..936)
                     /locus_tag="VF870_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251874.1"
                     /translation="MKVFVIAGLLVFVLMLITYTKSQPDFNGTSPGCGESGCHASEAG
                     IVSAEVLNNFQVRITVTGTTSKVGGELVNASGNVVAVINSTSSNPFTLTAPSAGTYTV
                     NAGFKNPSRKWGSTSTVINVTGIDEDLVGLRPEAFALYANYPNPFNPSTKIRYAISQT
                     AFTSLKVYSILGEEVATLINEEKTPGVYEVNFDATNLTSGTYLYKLQAGNYTETKKMI
                     ILK"
     gene            complement(1129..1920)
                     /gene="blaOXA"
                     /locus_tag="VF870_06510"
     CDS             complement(1129..1920)
                     /gene="blaOXA"
                     /locus_tag="VF870_06510"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HEX9251875.1"
                     /translation="MKQIQILLSFLVFITSTIFSQKVEERTDLKKYFDEYGHEGCFVL
                     YDLKQDLYLMYNPERCAERFIPASTFKIFNSLVGLETGAVKDEFEVFKWDSVKRFYNS
                     WNQDLDMVNAFKYSAVWFYQEIARKIGEEKMQHYISINHYGNENISGGIDRFWLDGGL
                     RISADEQIEMLKKLYHNKLQFSKRSMDLVKHIMIYYKTDEYTIRAKTGWALRVEDQIG
                     WFVGYVEKGEDVYFFAINLQSKKPEEGFVSRKEITFAILKHLGIL"
     gene            2005..>4650
                     /locus_tag="VF870_06515"
     CDS             2005..>4650
                     /locus_tag="VF870_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251876.1"
                     /translation="MFRQKFLTKDFTTKIIEKAAGKLPQNMLEDFLSSIEKETTRHYF
                     TKSSESNLLRIILNQFDIAFFINECLKYPHHIEILITLSNNSNYLTDILVRNPEYFHW
                     IINPSVLEQKIDRKYFQDSLEKTVVPFKSFASKLNAIRNFKRKEILRIGLKDIFLKED
                     LKKITSYLSNLANSISSILFDLCYKEILTKYEIEKTTRKYVLFSLGKLGGNELNYSSD
                     IDLVAFYDKNSLINKNIYYNQILSETVLLFIETASKITGAGFLYRVDFRLRPDGRNAP
                     LCGSYAEYLKYYETRGEDWERQMLIKASFLYGSKSLYDKFWHSISRFIYPVTFSVSPI
                     EQIRKLKKSIEQRNKSDENIKLVSGGIRDIEFSLQALQLLNGGKDIIVRTGNSLDAID
                     KLFNKKILSNEEAEVFIDAYNFYRKAEHYLQLMNDQQTHTIPAEGEIAEKLSHYLQFK
                     DLKSFKNYLNLSKEKVQSVYNSIIGINDLSDKKNDIDKIIFSDTKRAKSNFEFLRTGK
                     SLFDKKQFDNRTTSTFEKIADKLIEFLADSIDPDIVLENFARVIRTAQFPQIWFDEFG
                     DTKFFDLFLNLCEHSQKAIDLFAEDKILRDTFLSRVCLAPLKKDIISNLTLKDFYFRA
                     SVQLTAGIIKPESFADFYTEFLNQKIISLTNGFITERNWKDDFFIAAMGSFGSKELTF
                     ASDVDLIFVVSDIQNYAGIQKEFQKLLQIFKDNLPGIEIDCRLRPEGKSSQLVWDIED
                     YKKYFSNRARIWELQAFTKCRFVSGNADLFNSFYTHYIQTVSGKDSTTIKKEMFEMRK
                     KLYPAIDALFNIKKNSGGLADIDFIVSFYLLTNPDLLIVRNEKGIYNSFELIQKVSTK
                     EIIFNQLEENYYLLKKIELVNQLIFN"
BASE COUNT         1619 a          741 c          717 g         1573 t
ORIGIN      
        1 atttgaatca cttacgcgtg atgacgttga acatcccaga agcacgacaa ataaagacaa
       61 aatggttata aaagttttca taagattttt tatggtaatt attaatttat gtttcactaa
      121 aattatttaa caaacctgaa caactttata aaagaacata ctgaaattag tcaaaggtta
      181 aaaagactac aagaaatttt agatcagata aacaaaaaag gcgactcatt tgaatcgcct
      241 aattaaaaat agatcatcgg aagatcagat ttattttaag attatcattt tcttagtttc
      301 agtataattt cctgcctgca atttgtacaa atatgttccg ctggtaagat ttgtagcatc
      361 aaaattaact tcataaactc cgggagtctt ttcttcatta attagagtgg caacttcctc
      421 accaagaatt gaataaactt ttagtgaagt aaaagcagtt tgtgatatag cataacgtat
      481 ttttgttgat ggattaaatg ggttcgggta attagcatat agtgcaaaag cttctggtct
      541 tagtccaact aaatcttcat ctattcctgt tacattaatt actgtagatg tactacccca
      601 ttttcttgat gggtttttaa agccggcatt tacagtatat gttccggcag atggagctgt
      661 taaggtaaat ggattactgg aagtggagtt aataaccgct actacatttc cggaagcgtt
      721 aacaagctca ccaccaactt tgcttgttgt tcctgtgaca gttattctaa cctgaaaatt
      781 attaagtact tcagctgata caattcctgc ttccgaagca tggcaccctg attcaccaca
      841 tcccggggaa gtaccattaa aatccggttg tgattttgta tatgttatta acattaaaac
      901 aaaaactaat aaccctgcaa ttacaaatac tttcatcaca atctccttcg gaataatgac
      961 taaaagtttc caataactat gcctctgata aatagacaaa ttttaaaagt taaagagaat
     1021 atttttagtc atatgacaaa ttaggcgggt tatatttcca gcaggtattt tttacccttt
     1081 ctaaaaaata attttattca ttcggcttgt gcggattttt atcctgattt ataatatgcc
     1141 cagatgctta agaatagcga aagtaatttc tttccttgat acaaatcctt cctcaggctt
     1201 ttttgattga agatttatgg caaagaaata gacatcttct cccttttcaa cataaccaac
     1261 aaaccaccca atctgatcct caactcttaa tgcccagcca gttttagcgc gaattgtata
     1321 ttcatcagtt ttataataaa tcattatgtg ctttacgaga tccatacttc ttttagagaa
     1381 ctgcaatttg ttatgataaa gtttttttag catttcaatt tgttcatcag cggaaattct
     1441 gagcccgcca tctaaccaga atctgtctat tccgccggat atgttttcat ttccataatg
     1501 attaattgaa atataatgct gcatcttttc ctccccaatt tttcttgcaa tttcctgata
     1561 aaaccatact gccgaatatt taaaagcatt caccatatca agatcctgat tccagctatt
     1621 ataaaatctt tttacgctat cccatttgaa aacttcaaac tcatccttaa cagctccggt
     1681 ttccaatcct acaagcgaat taaatatttt aaatgtggat gcgggtataa acctctccgc
     1741 acatctctca ggattgtaca ttaaatataa atcctgttta agatcataaa gtacaaaaca
     1801 cccttcgtgt ccatattcat cgaagtattt tttcagatca gttctttctt caaccttttg
     1861 agagaatatt gttgaagtga taaagactaa aaaactaaga agtatttgta tctgtttcat
     1921 aatagagccg tattaagttt taagaaactt ttaattatgc ttggtttaat attagattcc
     1981 aatagtaaaa aaacaaaatt ctaaatgttc agacaaaaat ttctcacaaa agattttaca
     2041 accaagatca ttgaaaaagc tgcgggaaaa ttaccgcaaa atatgcttga ggattttttg
     2101 tcttcgatcg aaaaggaaac aacacgtcat tattttacaa aatcttccga atcaaatctt
     2161 ttaaggataa ttctaaatca gtttgatata gcttttttta ttaatgaatg tttaaagtat
     2221 ccgcatcata tcgaaattct aattactctg tctaacaaca gcaattacct tactgatatc
     2281 cttgttagaa atccagagta ttttcattgg atcataaatc catctgtgct ggagcaaaag
     2341 attgatagaa agtattttca agatagttta gaaaaaacgg ttgtcccttt caaatctttc
     2401 gcttccaaat taaatgcaat cagaaacttt aaacgcaaag agattttaag aataggatta
     2461 aaggatatct tcttaaaaga agatcttaaa aaaatcacaa gttatctttc aaatcttgct
     2521 aactccattt catcgatatt atttgattta tgctacaaag aaattttaac caagtatgaa
     2581 attgaaaaga caacgcgcaa atatgtttta ttttcactcg gtaaacttgg cggtaatgag
     2641 ttgaattata gttctgatat tgatctggtg gcattttatg ataagaattc tttaatcaat
     2701 aaaaatattt actacaatca gattctttct gaaactgttt tactttttat agaaacagca
     2761 agcaaaataa caggtgcggg atttttatat agggtggatt ttcgtcttcg tcctgacgga
     2821 agaaatgctc ctttgtgcgg tagctatgcc gagtatttaa aatactacga aactcggggt
     2881 gaagattggg aaagacaaat gcttatcaaa gctagctttc tttacggaag taaaagctta
     2941 tacgataaat tttggcattc tatttcaaga tttatttacc cggtaacttt ttcagtctct
     3001 ccaattgaac aaatcagaaa gcttaaaaag agtattgagc agagaaataa atctgatgaa
     3061 aatattaaac ttgtttctgg tgggataaga gatattgagt tttccctgca agcacttcaa
     3121 cttctaaatg gcggtaaaga tataatagta cgaacaggca attctttaga tgccattgat
     3181 aaattattta ataaaaaaat cctttctaat gaagaagcag aagtttttat tgacgcttat
     3241 aatttttaca gaaaagcaga gcattatctt caattaatga atgatcagca gacgcatact
     3301 atacccgctg aaggcgagat cgctgaaaaa ctctcccact atttacaatt caaagatttg
     3361 aaatcattta aaaactattt gaatctttca aaggaaaaag ttcagtccgt ttacaattca
     3421 attattggga ttaatgattt atcagataag aaaaatgata ttgacaaaat aatattttca
     3481 gacactaaaa gagcaaaaag taattttgaa tttttacgaa ccggaaaaag tctttttgat
     3541 aagaagcagt ttgataatcg tacaacttct acttttgaaa aaattgcaga taaattaatt
     3601 gaatttttag ctgattcgat tgatcctgat atagtattag aaaactttgc acgagtaatc
     3661 cgcacagcac aatttccaca gatttggttt gatgaatttg gagatacaaa attctttgat
     3721 ttgtttctga atctctgtga acattcacaa aaagctattg atctttttgc agaggataaa
     3781 attttgcggg atactttttt gtctcgtgta tgcctggcac cgctcaaaaa agatattatt
     3841 tcaaatctta ccttaaaaga tttttatttt agagcctctg ttcaattaac cgcaggaatt
     3901 attaagcctg aatcatttgc agatttttat acagaatttt taaatcaaaa aatcatctca
     3961 ttaacgaatg ggtttattac ggaaagaaac tggaaagatg atttttttat tgcagccatg
     4021 ggaagttttg gttctaaaga attaacattt gcttcagacg tggatttgat ttttgttgtt
     4081 agcgatatcc aaaattatgc aggcatccag aaagaatttc aaaaactatt acagatattt
     4141 aaagacaact taccaggaat tgaaattgat tgcagactaa gaccagaggg caaaagcagt
     4201 caactagttt gggatattga agattataag aaatattttt ctaaccgtgc aagaatttgg
     4261 gaactgcagg cttttactaa gtgccgattt gtttctggca atgcagattt gtttaacagt
     4321 ttctatactc attatatcca aacagttagt ggaaaagact ctacaacaat aaaaaaagaa
     4381 atgtttgaga tgcgcaaaaa actttatccg gcaattgatg cattatttaa tattaaaaaa
     4441 aactccggtg ggttagcaga tattgatttt attgtttcat tttatttact gacaaaccct
     4501 gatttattga ttgtgagaaa cgaaaaaggg atttataatt cttttgagct gatacaaaaa
     4561 gtttccacta aagaaattat ttttaatcaa ctggaagaaa attattattt actaaagaaa
     4621 attgaattgg ttaatcaact aatatttaat
//
LOCUS       DASWBY010000351         4508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_16258, whole genome shotgun sequence.
ACCESSION   DASWBY010000351 DASWBY010000000
VERSION     DASWBY010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16258"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VF870_06520"
     CDS             complement(<1..903)
                     /locus_tag="VF870_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251877.1"
                     /translation="MTVAYLNKITVLILFAFSSLVIAQQKLKTEANADLIEYTNKEGL
                     PTTNITNGIQTKDGFIWISSVEGTYRFNGYEFEEVGTDIGLPAMQNMYYDSTKNVMYF
                     ASPTKFITFDGKDFKVYTEKEGYKINGLEGQLITFVGSDSKGRIWIGSSTPFVDKNNN
                     GGLTKFENGKFTVYDSKSFPLDNATNFIETPYGDLIFNSAGRNTQTREGSYVALFKNG
                     VFKRIDESVGITLQNASMFPEENVTAIDRDGNTWMACSGVIGFSEESKNYSGVLMYDG
                     NKFYQFTEFKNQFKKEQVPIQVYYS"
     gene            complement(985..>4508)
                     /locus_tag="VF870_06525"
     CDS             complement(985..>4508)
                     /locus_tag="VF870_06525"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9251878.1"
                     /translation="DPNDSTTIPTNDCSAIAEDADGFIWIGVSGNAITRYNPKNETFQ
                     SYRIETAGVTNISYFYSALFDSKGNLWFGSSNHGMQKLNRSKNRFEQVRLDSSNNIAQ
                     WGQIYSITELKNGNILVADYGNGIKTYNEKLNIFQSYYLNTNFSPNELQVIYEDVSGN
                     IWFGGRNQLIRYSTSFYTIDNYDLFSLIKNPTSYDNVTGITQDDEGYLWAGVYSQGLF
                     RIEPKTKNIQKFDYNIYDLNNFGRNIISNIYKDRYGVIWIGIWGKGITKFDPLREPFN
                     YQKFITNDIASSNANFSTVIAGLHQSKQITVGTSEKGLFTYNLENQKSDKLNLHLIKS
                     NNSDGKINIQGLAIDNEGNKWLAFNNLGLHKIDKNNLLSSIKSPNENKTSVYFINTIK
                     IDLMGNIWMASRQGFERYNPMQNKYSLLPTIMNKPLSEDLKQKIHEISESGDPIASLL
                     KVGEASNLEKTFSLNRDQNVLIICVGEGEMNVSLGVSDAGSLLSGDGKTIWSMNDLYK
                     TFNDGGGFKNRIALGCFNLKKGNYKITYATDVGHSYGNWNAIAPPDSNWYGIQVLRLD
                     ESDFNGLNEMIEKEINSDKFMPMEVGTSIEFSKRFNNVLWLGSTISGFFKYDLASGNF
                     KQYNFDSKNIFSPNNYINCIFEDSKGIVWIATANSLLRFDPESEKIDKYDQKDGLPSN
                     QINSIIEDLEGNLWINTSAGLSKLNKTTSKDKWNFVNFDTRDGIQAFTTSKANWISKD
                     GEIFLGSIDGITYFYPGKINEVKPDIVIDDIKINEISLKSDSSIAKLEQSIMKTEELV
                     LSSSQNNISFEFASIHFSRPEKNKIIYMLEGFNDHWIAADRNFAAYTNLAPGEYTFMV
                     KGSNGDGIWNDEGKAIRIIINPPWWRTTFAYIGYFFFFVGIIFGIDRIQRRRIVNKER
                     NAAAIKEANLRAQLAEAENERKSQELEEARQLQLSMLPKVLPQLPHLDIAVYMKTATE
                     VVGDYYDFNVSLDGTLTVVLGDATGHGMRAGTMVTSAKSLFNSYAQNSDILFTFREMT
                     RCIKQMQFQSLAMSMMMLKIKNNKLMMSSAGMPPVYLFRNKHKIIEEYLIEGMPLGTM
                     DNFPYELKEMDLFSGDTILLMSDGFPELNNQQNEMFGYRRARNSFEEVAEKEADKIIE
                     YLKNEGNRWANDNDPDDDVTFVVIKVK"
BASE COUNT         1379 a          864 c          632 g         1633 t
ORIGIN      
        1 actatagtat acttgaattg gaacttgttc ttttttaaac tgatttttga attctgtgaa
       61 ctgataaaac ttattaccat cgtacatcaa aactccagaa taatttttgg attcctcaga
      121 aaaacctata acaccagagc aagccatcca tgtattccca tccctatcaa ttgcagttac
      181 gttttcctct gggaacatac ttgcgttctg taatgttatt ccaacagatt catcaattct
      241 tttaaacaca ccgtttttaa acaaagcgac ataagagcct tcacgagttt gagtgttacg
      301 accggctgaa ttaaatatca gatcaccata gggagtttca ataaagtttg ttgcattatc
      361 tagaggaaaa ctctttgagt catatactgt aaattttcca ttttcaaatt ttgtcagacc
      421 gccattatta tttttatcca caaatggtgt tgaagaacca atccatattc ttcctttact
      481 gtcggatcca acaaaagtta ttaactgtcc ttctaatccg tttattttgt aaccttcctt
      541 ttcagtatat actttaaaat cttttccatc gaatgttata aattttgtgg gcgaggcgaa
      601 atacattaca tttttagtcg aatcgtaata catattttgc attgcaggta atccaatatc
      661 agtgcctact tcttcaaact catatccatt aaacctgtat gttccttcaa cactggaaat
      721 ccaaataaaa ccatctttag tttgaattcc atttgtgata tttgtggtag gcaaaccttc
      781 tttgtttgtg tactcaatta aatctgcgtt ggcctcagtt tttagctttt gctgcgctat
      841 taccagcgaa gaaaaggcaa atagaataag gactgttatt ttatttaaat atgctactgt
      901 cataaaacgc tccgtatata agattttaaa tttcaatgac catcaagtag acgcaattca
      961 gctaaataaa ttgattacaa aaaattattt tacttttatc acaacaaatg ttacatcatc
     1021 gtccgggtca ttgtcgtttg cccagcgatt accctcattt ttcagatatt cgataatttt
     1081 atctgcttct ttttctgcta cttcttcaaa agaatttctt gcccgtctgt aaccaaacat
     1141 ttcattctgc tgattgttta gttccggaaa accatcgctc attaataaga ttgtatctcc
     1201 tgaaaacaaa tccatttctt ttaattcata tggaaagtta tccattgttc ccaatggcat
     1261 tccttcaatt aaatattctt caattatttt atgcttattt ctgaaaagat aaacaggcgg
     1321 cataccagca gatgacatca ttagtttatt gttctttatc ttcagcatca tcatactcat
     1381 tgctagcgac tgaaactgca tctgcttaat gcatcttgtc atttcacgga atgtaaaaag
     1441 tatatccgaa ttttgtgcat aactattaaa taatgactta gcggaagtaa ccattgttcc
     1501 agctctcatt ccgtgtcctg ttgcatcacc taacacaact gttaatgttc catcaagtga
     1561 aacattaaaa tcgtaatagt caccgacaac ttccgttgca gttttcatat aaacggctat
     1621 atcaagatgc ggtaattgag gcagtacttt cggaagcatt gataactgca gttgtcttgc
     1681 ttcttcaagt tcttgtgatt tgcgttcatt ttcggcttca gcaagctgtg cacgcagatt
     1741 agcttcttta attgcagccg cattacgctc tttgtttaca attcttcttc tttgaatcct
     1801 atctatccca aatattattc ctacaaaaaa gaaaaagtat cctatgtatg caaaagtcgt
     1861 tctccaccag ggcggattta taataattcg aattgctttt ccttcatcat tccaaatccc
     1921 atcaccgttt gaacctttta ccataaaagt gtattcacca ggggcaagat ttgtgtaagc
     1981 agcaaaattt ctatcagcag ctatccagtg atcattaaat ccttctagca tatagatgat
     2041 tttatttttt tctggtcttg aaaaatgaat agatgcaaat tcaaaagaaa tattattctg
     2101 agaagaagaa agaaccaact cttcagtttt cattatactt tgttccaatt tcgcaataga
     2161 agaatctgat tttaaagaaa tttcattgat cttaatatca tctatcacta tatctggttt
     2221 aacttcattt atttttccag gatagaaata agtaattcca tcaatacttc ctaaaaatat
     2281 ctctccatct ttacttatcc agtttgcttt acttgtagtg aaggcctgaa ttccgtccct
     2341 tgtgtcaaaa ttaacaaagt tccatttatc cttcgaagtc gttttattca gtttggataa
     2401 tccggcagaa gtatttatcc aaagatttcc ctcaagatct tcaataatcg aatttatttg
     2461 attggaagga agtccatctt tttgatcgta cttatctatt ttttcactct caggatcaaa
     2521 tcttaataaa ctatttgcag ttgcaatcca gactattcct ttactatcct caaaaatgca
     2581 attaatataa ttgttgggag aaaaaatatt ttttgaatca aaattgtatt gcttaaaatt
     2641 tcctgatgcc agatcatact taaaaaatcc gcttatagtt gagcctaacc agagaacatt
     2701 attgaaccgc ttactaaact caatactagt tcctacttcc atgggcataa atttatctga
     2761 gttaatttct ttttcaatca tttcattcag gccgttaaaa tcggattcat ctaatctcaa
     2821 aacctgaatt ccataccaat tagaatctgg tggtgcaatt gcgttccaat ttccgtaaga
     2881 atgtccaaca tccgttgcat atgtaatttt ataattgccc tttttcaaat taaaacagcc
     2941 tagtgcaata cgatttttaa atccccctcc gtcattaaat gttttatata aatcattcat
     3001 agaccatatt gtttttccat ctccacttaa gagtgacccg gcatctgaaa cacccaaact
     3061 aacattcatt tcaccttcac caacacaaat tattaaaaca ttttgatccc gattcaatga
     3121 gaatgttttt tctaaatttg aagcttcccc aacttttaat agtgaagcta ttggatcacc
     3181 cgattctgat atttcgtgta ttttttgttt aagatcctca cttagtggtt tattcatgat
     3241 cgtaggtaaa agtgaatatt tattttgcat agggttatat ctctcaaaac cctgtcttga
     3301 tgccatccag atattaccca ttaaatctat ttttatcgtg tttataaaat aaactgaagt
     3361 tttattttca tttggtgact taatactgct taacaaatta tttttatcaa ttttgtgcaa
     3421 accgagatta ttaaatgcca accatttatt cccctcatta tcaattgcaa gaccctgaat
     3481 atttatcttc ccatctgaat tattagattt aatgagatgt aaattcaatt tatcagattt
     3541 ttgattttct aaattgtaag taaataagcc cttctcagat gttccgactg ttatttgctt
     3601 actttgatgt aacccggcaa ttacggttga aaaatttgca ttagaacttg caatgtcatt
     3661 ggttataaac ttctgataat taaacggttc tctaagaggg tcaaattttg taataccttt
     3721 cccccaaata ccaatccaga ttactccgta cctgtcttta taaatattag aaataatatt
     3781 gcggccgaag ttgtttaagt cataaatatt gtaatcaaat ttctgaatgt tttttgtttt
     3841 cggttcaatt ctaaaaagtc cttgtgagta gactccggcc cacaagtatc cttcatcatc
     3901 ctgagttatt cctgtaacgt tatcgtaact ggtgggattt ttaattaagc taaataaatc
     3961 gtaattatcg atcgtataaa aagacgttga atatcttatt aattggtttc tgccgccgaa
     4021 ccaaatattg ccggacacgt cttcatatat aacctgtagt tcatttgggc tgaaatttgt
     4081 attcaaataa taagactgaa atatattcag tttttcgttg taagttttaa tgccgttacc
     4141 gtaatcagca actaaaatat ttccattctt tagctcagtt atactatata tttgtcccca
     4201 ttgagcaatg ttattagaag aatctagacg tacctgctca aatctgtttt tcgatctatt
     4261 caatttttgc atcccgtgat ttgaggagcc aaaccacaaa tttcctttag aatcaaaaag
     4321 agccgaataa aagtatgata tgttagtaac tccggcagtt tctatacgat agctttgaaa
     4381 ggtttcattt ttgggattgt atcttgtaat agcattcccg gaaacaccaa tccagataaa
     4441 cccatctgca tcctcagcaa ttgcagaaca atcattagtt ggtattgtag tggaatcatt
     4501 tggatcgt
//
LOCUS       DASWBY010000352         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_16321, whole genome shotgun sequence.
ACCESSION   DASWBY010000352 DASWBY010000000
VERSION     DASWBY010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16321"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..958)
                     /locus_tag="VF870_06530"
     CDS             complement(107..958)
                     /locus_tag="VF870_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9251879.1"
                     /translation="MSNKIINRQDITLSEQQQKMIVSGWGRDTLEKSVVEKITYLSDG
                     LKVSGYIAYPKDDSKKYPCIIWCRGGIGNAGAIDKFTARGIFGQLASWGYCVFASQYR
                     GNDGGEGHDDFGGDDLNDVLNLIPLADEIPQANKDMWGIEGWSRGGMMTYLALTKTNI
                     FKTAIVLGGIANLRCNSDESKFMRRLYEHTLGRYEESEFKSRCETRSIVNFPEKLSKT
                     TPMLIIHGNADNRVLPHDSLDLSYKLLDLKIPYRLVMLESGDHFLKSHRKEVDEMRRN
                     WFEQYLK"
     gene            complement(967..1605)
                     /locus_tag="VF870_06535"
     CDS             complement(967..1605)
                     /locus_tag="VF870_06535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX9251880.1"
                     /translation="MINENQSIPLVSIIMPVYNRQAYIKRAIDSVLNQSYVNWELITI
                     DDGSQDYSPYLLEIYRAYSHKIKVVYQNHQHLPAARNNGIKNSVGEFITFLDSDDEIA
                     KDHIEQRINYFAQNSEIDLVHGGVKIIGDPFVPDKDDHTKLIHLSDCTIGATFFGKRS
                     VFLDSGGFKDIPYSEDSEFLERIVKKYRVSKVDFPTYVYHRETPDSITNSIK"
     gene            1757..2314
                     /locus_tag="VF870_06540"
     CDS             1757..2314
                     /locus_tag="VF870_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX9251881.1"
                     /translation="MAKKQIKQSEQKTINKKSKLTLQKVVIAVIALAVIGFFIVNNFI
                     KHEPKVEYYTFTKEGELTFTDSTGVLKAKIDLEIADNEYERELGLMNRNEMKENQGML
                     FIFPAQRLQSFWMRNTLISLDMIFADEHKKIVTIHKNTKILSDTSYPSSQPSMYVVEV
                     IAGYTDKHNIQVGDKIDWIGTRVGL"
BASE COUNT          788 a          424 c          357 g          782 t
ORIGIN      
        1 ttggcgtgtt tactttgcga actttgcgtg aaatgcaact aaaagacttc acgcaaagaa
       61 cgacaagatt tttcgccaag atcgcaaagt caaagataag agttaactac ttcaaatatt
      121 gttcaaacca atttcgtctc atttcatcaa cttctttacg atgggatttt aggaaatgat
      181 ctccactttc cagcattacc aatctatagg gaatctttaa atccaaaagt ttgtaagaaa
      241 gatcgagtga atcgtgcggt aacaccctgt tatctgcgtt tccgtgtatg attaacatcg
      301 gcgtagtctt ggaaagtttt tcaggaaagt ttacaatcga tctggtttcg catctgcttt
      361 taaattcaga ttcttcatat ctgcctaaag tatgttcata aagcctgcgc ataaatttgc
      421 tttcatcaga gttgcagcga agatttgcaa ttccgcctaa tacaattgcg gttttaaaaa
      481 tatttgtttt agttaaggct agataagtca tcattccgcc acgactccag ccttcaattc
      541 cccacatatc tttatttgcc tgtggaattt catcagcgag tggaataaga tttagaacgt
      601 catttaaatc atctccgcca aaatcatcgt gaccttcacc gccatcgtta ccgcgatact
      661 gcgaagcaaa aacacaatat ccccagcttg caagctgtcc aaaaattccg cgggcagtaa
      721 acttatcaat cgcacccgca ttaccgattc ctcctctgca ccagataata cagggatatt
      781 tttttgaatc atcttttgga taagcaatat aacctgaaac ttttaatcca tcagaaagat
      841 atgttatctt ttcaacaact gatttttcaa gagtatctct tccccatccg ctaacaatca
      901 ttttttgctg ctgttcgctt aaggtaatat cctgcctgtt aataatttta ttactcattc
      961 taattcttat ttaattgagt ttgtaatact atctggtgtt tcccggtggt acacataagt
     1021 tgggaaatca actttactaa ctctatattt tttaactatt cgttcaagaa attcagaatc
     1081 ctcgctgtac ggaatatctt taaacccgcc tgaatcaaga aaaacacttc tctttccaaa
     1141 aaaagtcgca ccaattgtac aatcagatag gtgaattaac ttagtatgat catctttatc
     1201 tggaacaaat ggatcaccaa ttattttaac accgccatgt acaagatcta tttcagaatt
     1261 ttgggcaaaa taattaatcc tctgttcaat atgatcttta gcaatttcat catcgctgtc
     1321 taaaaaagta ataaattctc caacagaatt tttaattcca ttatttcttg ctgccggaag
     1381 atgctgatga ttttgatata ccactttaat tttgtgtgaa tatgcacgat agatttcaag
     1441 aagataaggc gaataatcct gacttccatc atcgattgtt atcagttccc aattaacata
     1501 ggattgatta agtactgaat ctatagctct tttaatataa gcttgtctgt tataaacagg
     1561 cattattata gaaacaagcg gtattgattg attttcgttt atcaaattgt tgcctatcta
     1621 taaatacttt aaaatctgtt ttaattgctg tataaattta cattaccaaa taaacatatt
     1681 ttagagacaa aagtttcaaa tatttaataa cactgtttta ttatatttga actatataaa
     1741 tatcggaaaa atgataatgg ctaaaaagca gattaaacaa tcagaacaaa aaacaattaa
     1801 taaaaaaagt aaattaactt tacaaaaagt tgtaattgca gtaattgcac tggcagtaat
     1861 agggtttttt attgtaaata attttataaa acacgagcct aaagtggaat actatacttt
     1921 tactaaagaa ggtgaactta cctttactga ttcaaccggc gtattaaaag ctaagattga
     1981 tcttgaaata gccgataatg aatacgaaag agaattgggt ttgatgaaca gaaatgaaat
     2041 gaaagaaaac cagggaatgc tttttatttt tcccgcacaa agacttcaat ctttctggat
     2101 gcgaaatact cttatttcac ttgatatgat atttgctgat gaacataaga agattgttac
     2161 aattcacaaa aacacaaaaa tactttctga tacaagctat ccttcatctc agccttcaat
     2221 gtatgttgtt gaagttatag ccggatacac cgacaaacat aatatccagg tcggtgataa
     2281 aattgattgg ataggtacca gggttggctt ataaaaatta atttcgtttt ttaattacaa
     2341 ctcttaaaag t
//
LOCUS       DASWBY010000353         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_239160, whole genome shotgun sequence.
ACCESSION   DASWBY010000353 DASWBY010000000
VERSION     DASWBY010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239160"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="VF870_06545"
     CDS             <1..1503
                     /locus_tag="VF870_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9251882.1"
                     /translation="VKMFSLTDISGNKNDKVLLDSHLNAHFQTGFVNPIDQAIINYQE
                     INLNDYKKTDEIPYDFLRKRLSVLVSTKDSNIIITKGALKNVLEVCDYAEDENGATID
                     IKLNIEKINNYYVDMSRNGYRVLGIAYKNIPPAISLKRDLEKGMTFLGFIILFDPPKQ
                     NIIETISNLSKLGVSLKIITGDNKLVAENVSKQIGLKDTKIVTGMDIRKLSDESLYRS
                     VNDINIFAEIEPNQKERIILALKKAGNVVGYMGDGINDASALHAADVGISVDSAVDVA
                     KEASDIVLLETDLNVLLSGIREGRMTFANTTKYVFMATSANFGNMFSMAGASLFLPFL
                     PLLPKQILLTNLLTDFPEMTIATDNVDPDLIEKPRRWDIKFIRKFMLIFGIVSSVFDY
                     LTFGILLWVLDANMEQFRTGWFIESVISAVLIVLVVRTSKPFYKSKPGKYLIIATTAI
                     ILFTIIIPVSPLIKLFEFISLPIIFIPYIVVIILFYIFTAEVAKKFFYRD"
     gene            1509..2117
                     /locus_tag="VF870_06550"
     CDS             1509..2117
                     /locus_tag="VF870_06550"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9251883.1"
                     /translation="MESVLHLINEYGYLGLYISMTITIIGIPIPGESILTFAGYLAFQ
                     KDLYLIPTIISASLGSFTGVTISFFLGRILSDKLLNKRKTFFSISESNLIKTKKWLEN
                     YGSWILMFGYYIPGVRHLTAIVAGSTQIKYSRFALFAYIGGFLWSLTFILLGYYVGKK
                     WMSILAQIHNHIFIITFSSLSLIILYFIFKLTYSKKNATKKF"
     gene            2160..2552
                     /locus_tag="VF870_06555"
     CDS             2160..2552
                     /locus_tag="VF870_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX9251884.1"
                     /translation="MITKNPNNIYKSILSESEKFAIWITDKVGTIGFFAIIFFWTLIW
                     LSWNMFAPRELRFDPYPAFVLWLFISNMIQIFLMPLIMVGQNLQSKHAEIRAEIDFEV
                     NVRAEKEIAELRNDLNEIKDLLNKLLLK"
     gene            2579..3439
                     /locus_tag="VF870_06560"
     CDS             2579..3439
                     /locus_tag="VF870_06560"
                     /inference="COORDINATES: protein motif:HMM:NF025123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEX9251885.1"
                     /translation="MTVKKLFKVYKKTLIIILLLVIVENVAWIIEPTVFGNVIDEFIN
                     KSYSRNLRFQSHHMQLLLMWILLYAINSISGTVRRRMEPKVFQNMNTDIAIKISEAVR
                     ENKIEPEKGAARAQLAEQIVSFFQFRIPEIIEQSITFGGAIIALTFIDWRISVVCLLV
                     GVPLIVINIIYNKKVSVLQTELHDNYENVYETFSTKNPENVKNRFNKMGSVQRKIGNW
                     SAINFGSLRLILLIIFIFVLYIAIDLDDFTTGKIYSIVAYLWSFIGTVEYLPDLMESA
                     ASLKDIKNRI"
BASE COUNT         1230 a          469 c          590 g         1202 t
ORIGIN      
        1 gtaaagatgt tttctctcac agatatttcg ggcaacaaaa atgacaaagt gcttttagac
       61 tctcacttaa atgcacactt ccagactggt tttgttaacc cgatagatca ggcaataatt
      121 aactaccagg aaattaatct gaatgattat aaaaaaacag atgaaattcc ttatgacttt
      181 ttaagaaaaa gacttagtgt actcgtttca actaaggaca gcaatattat aatcacaaaa
      241 ggcgcactta aaaatgtatt agaagtatgt gattatgcag aggatgaaaa tggagcaaca
      301 attgatatta aattaaatat cgaaaagatt aataattatt atgttgatat gagtagaaat
      361 ggttacagag ttttaggcat tgcatataaa aatatacctc cggcaatcag cttaaaaagg
      421 gatcttgaaa aaggaatgac atttttaggg ttcattattt tattcgatcc gccgaagcaa
      481 aatattattg aaacaatatc aaaccttagt aagctgggcg tttctctaaa aattattaca
      541 ggagataaca aactagttgc tgaaaatgtc agtaagcaga ttggcttgaa ggatacaaaa
      601 attgtaacag gaatggatat tcgaaaactt agtgatgaat ctctatatag aagtgttaat
      661 gatataaata tttttgctga aattgagccc aaccaaaaag aaagaataat tttagcatta
      721 aaaaaagcgg gtaatgttgt tggatatatg ggcgatggta taaacgatgc ttcagcttta
      781 catgctgcgg atgttggaat atctgtggac agtgctgttg atgtggctaa agaagcttca
      841 gacattgttc ttcttgaaac agatttaaac gttcttttaa gcggaatccg tgaaggcaga
      901 atgacttttg ccaacactac caaatatgtt tttatggcta caagtgcaaa ctttggtaat
      961 atgtttagta tggcaggcgc atcattgttt ctaccatttc ttccgcttct tccaaaacaa
     1021 atactgctta caaatttact aactgatttt cctgagatga ctatagcaac tgataacgtt
     1081 gatccggact tgattgaaaa accaagacga tgggatatta aatttataag aaaatttatg
     1141 ctgatttttg gaattgtaag ttctgtgttt gattacctga cttttggaat tttattatgg
     1201 gttttggatg caaacatgga gcaattcaga acaggatggt ttattgaatc agtgatttcg
     1261 gcagtactta ttgtactagt tgtacgaaca agtaaacctt tttataaaag taaacctggc
     1321 aaatatctta ttattgctac tactgcaatt attttattta caattataat tcctgtatca
     1381 ccattaataa aattatttga atttatttct ctgcctataa tttttattcc ttacatagtt
     1441 gttattattc tgttttacat ttttactgca gaagtagcta aaaagttctt ttatagggat
     1501 taaaaatatt ggaaagtgtt ttacatctaa tcaatgaata cggttatttg ggattatata
     1561 tctcaatgac tataacaatt attggaatac caattccagg tgaatcaatt ctcacgtttg
     1621 caggatatct cgcattccaa aaagatttgt atttaattcc tacaattata tctgcctctt
     1681 taggtagttt tacgggcgta acaattagtt tttttttagg acgaatactt agtgacaaac
     1741 tgctgaataa acgcaaaaca ttttttagta tatcagaaag caatttgata aaaacaaaaa
     1801 aatggttgga aaattatggc agttggattt taatgtttgg atattatata cctggtgtga
     1861 gacatttaac tgccatcgtg gcaggaagta cacaaatcaa atattccagg tttgctttgt
     1921 ttgcttatat cggcggattt ttatggtcat tgactttcat ccttttagga tattatgtag
     1981 gaaagaagtg gatgtctatt cttgcacaaa ttcataatca tatctttata attacgtttt
     2041 caagtctctc tttaataata ctctatttta tttttaaatt aacttattca aaaaaaaatg
     2101 ccacaaagaa gttttaagat tgataaataa ataattctaa ctaataaata ttaaacatta
     2161 tgattacgaa aaacccaaac aatatttata aatcgatatt aagtgaatcg gaaaaatttg
     2221 caatctggat aaccgacaaa gttggtacaa taggattttt tgcaattatt tttttctgga
     2281 ctttaatttg gctaagctgg aatatgtttg ctccacgtga attaagattt gatccatatc
     2341 cggcatttgt tctctggtta ttcatctcaa atatgataca gattttttta atgcctttaa
     2401 ttatggtagg acaaaatttg caatccaagc atgctgaaat tcgagcagaa attgattttg
     2461 aagtaaatgt tagagcggaa aaagaaattg cagagctaag gaatgattta aatgaaataa
     2521 aagatttgct taataaactt cttttgaagt aatagatcca aaatagttag aaataaatat
     2581 gacggttaaa aagctattta aagtatataa aaaaactctg ataataattc tattattggt
     2641 tatcgtagaa aatgttgcgt ggataattga accaacagtt tttggaaatg taattgatga
     2701 atttataaat aaatcataca gccgtaatct tcgttttcag tcacatcata tgcaattatt
     2761 gctgatgtgg atattgttat atgcaataaa ttcaatttcc gggacggtta gaagaagaat
     2821 ggaaccaaaa gtttttcaga atatgaatac agatattgca attaaaattt cggaagcagt
     2881 cagagaaaat aaaattgaac ctgaaaaagg tgcagcccgc gctcagctag cagaacaaat
     2941 tgtttctttt ttccaatttc gtatccccga aattatagaa caatctataa cattcggcgg
     3001 tgcaataatc gcactaacat ttattgattg gagaatttct gtggtttgcc tgttggttgg
     3061 tgtccctcta attgttatta atataattta caataaaaag gtttctgtat tacaaactga
     3121 gcttcacgat aattatgaaa atgtttatga aactttttca acaaaaaatc ctgaaaatgt
     3181 taaaaataga tttaataaaa tgggcagtgt gcaaaggaaa attggtaatt ggagcgctat
     3241 aaattttgga agcctcagac ttatacttct aataatattt atttttgtct tgtacattgc
     3301 tatagatctt gatgatttta caaccgggaa aatttattcg atagttgctt atctatggag
     3361 tttcattggt actgttgagt atttacccga tttgatggaa agcgccgcat cacttaagga
     3421 tattaaaaat agaatttaaa ttctgaatgc aaatattata tttaataaaa cataattaac
     3481 acttcattat t
//
LOCUS       DASWBY010000354         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_388756, whole genome shotgun sequence.
ACCESSION   DASWBY010000354 DASWBY010000000
VERSION     DASWBY010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388756"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..1681)
                     /locus_tag="VF870_06565"
     CDS             complement(320..1681)
                     /locus_tag="VF870_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9251886.1"
                     /translation="MDCSKHLAFGYVDMPPLTPFFAKLTIILLGETLQGLRFFPALLS
                     SIIVFLTGLITKEMGGKTFAQILASLTIIVSPIYLVSGTQFQTIPVDQFFWVLTCYLF
                     IRYINTNNQRLWLLIGFVVGLGLLAKYSIAFLAFAIFIGILTSKQRKLFTGKWIWFGT
                     LIGLFIFLPNIIWQIQNGFPVLEHIQALRKEESTPTLQFLIEQILIINPLTLPIWVAG
                     ILFFIFSEQVRKYRILVWIYFIPLLAFLLMKGQSYYMGPAYPILLAAGSVLLEIKLLQ
                     KQINWLKYAIPILLIVSSVITSPLWLPVLSIEKMKKFGIADISYDFREMIGWPEFVSS
                     VSTVYENLPNEDKKNTIIITGNYGEAGSINHYGSKLGLPVAASGIGSYYYWGPGNPDA
                     TTVLFVGYSEDYLKRYFSDVTVKKVFRNKYGINNEEQGILIILCRKPNKPILEMWPEF
                     KHF"
BASE COUNT          698 a          378 c          422 g          617 t
ORIGIN      
        1 cctgaccgaa cgaagtgagc gtcagggtgt cgcggtacaa ttttgatcgg agccgcaaag
       61 ttatccccgg taacaaaatt ggtagcgggc actggcgcag tgtcgcggta caaattatct
      121 cggagcctga aatttgtcaa ggtgcaggat ttaaattgcg tgtagatgag cgtgtcctgg
      181 tgcaacggcc attacgaatt tgtcttgagt gcaggagtaa aagcaaaaag tgtgacaaac
      241 aaatgacctt tattgtccgt gttagccgaa ggcctatttt tgttatttaa aagaaaatca
      301 tctgtcgggt tagaagaatt caaaagtgtt taaactcagg ccacatttct aaaattggct
      361 tgtttggttt acgacaaaga ataattagta ttccttgttc ttcgttattt attccgtatt
      421 tattcctgaa tactttcttt actgtcacat cggaaaaata cctttttaag taatcttcag
      481 aataacccac aaataataca gtcgtagcat ctgggttgcc aggtccccag taataatagc
      541 tgccaatacc acttgctgcc acaggtaaac caagctttga tccgtaatga ttaattgaac
      601 cagcttctcc ataattcccc gtaattatta tggtattctt cttgtcttcg tttggcaagt
      661 tttcataaac tgttgaaact gaactaacaa attccggcca tccaatcatt tcacgaaaat
      721 cgtaactgat atccgctatt ccaaattttt tcatcttttc aattgataaa acaggaagcc
      781 ataaaggact agtaatcact gaacttacaa tcaatagtat ggggatggca tattttagcc
      841 agtttatttg tttttggaga agttttattt ccaataatac cgaaccagca gctaataaga
      901 tcggataagc cggtcccata taatatgatt ggcctttcat taatagaaaa gcaagtaaag
      961 gaataaaata gatccatacc aatatcctgt actttctaac ttgctctgaa aatataaaga
     1021 ataaaatacc ggcaacccag ataggaagag ttaaaggatt tataataaga atctgttcaa
     1081 ttaaaaactg aagagttggt gtgctttctt ctttacgtaa cgcttgtata tgttcgagaa
     1141 cagggaatcc attttggatc tgccaaatta tattgggcag aaatataaaa agaccgatca
     1201 aagttccaaa ccaaatccac ttcccagtaa aaagttttct ttgtttgctt gttaaaattc
     1261 caataaaaat ggcaaatgcc aaaaaagcaa ttgagtattt agctaataag ccaagtccaa
     1321 caacaaatcc aatgagcaac caaagtcttt gattgttggt attaatgtat cttataaaca
     1381 gataacaggt aagaacccaa aagaattggt ccactggtat tgtttgaaac tgtgttccag
     1441 atactaaata aattggagaa acaataatag ttagggaggc aaggatttga gcaaatgttt
     1501 taccgcccat ttctttagtt atgagaccgg tcaggaatac aatgatagaa cttagcaaag
     1561 caggaaagaa tcttaaacct tgaagagtct cacctagcag gattattgta agctttgcaa
     1621 aaaatggagt taacgggggc atgtctacat aaccaaaagc aagatgttta gagcaatcca
     1681 ttaaatagag ctcgtcccgg tgaaagccat actggccatt cgtaaaaaga tgaatcagta
     1741 gatttgaaag tgatattcca agtaatatta agatatctcg gttcctaata tggagtttta
     1801 acatttccat ttgaagctat tgtcaaacat aaataatatt tctagtctaa tgcaagtttg
     1861 gtatcggctt acggctaacg gcccggcaat aaagggcgtt tgtgttggtt tgagaatgga
     1921 ggttgtgacc acaaatttgt ctaagaggca ggatcccggc acacaaccgg aatgaacaaa
     1981 ccatggcgca ctttttgcag tccgattaca aagttttatg agcatggcga gtgtcgcggt
     2041 acaagattga ttggaaacag gtaacaagtc aaggtacaca gagctttatt gagggcactg
     2101 gcgcggtcac ggtat
//
LOCUS       DASWBY010000355         4509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_199683, whole genome shotgun sequence.
ACCESSION   DASWBY010000355 DASWBY010000000
VERSION     DASWBY010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199683"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VF870_06570"
     CDS             <1..522
                     /locus_tag="VF870_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251887.1"
                     /translation="YKFWKSKKTEFINWLPFVDDEKELGIKYLQSAVKYSGYNSHLAI
                     HSLIWIYIEQKEFLKAIEVAELALKGHPQSRIFKWGLARSYENIDPAKSVLLYKEILY
                     SYPKDLNSNKINEVTLKHIIAQQLVKINKRDEALKICNEILSINGYSKYELDKLSNRL
                     DRVRDLKKELEKQ"
     gene            648..2789
                     /locus_tag="VF870_06575"
     CDS             648..2789
                     /locus_tag="VF870_06575"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX9251888.1"
                     /translation="MLDNLIKICKKNLPVVNEALIAKAFELSLEAHKNDIRASGEPYF
                     THPYEVAMIVAEEFPLDDVTIVTALLHDVVEDTEYSIEFMTREFGKEVSEIIDGVTKI
                     SGIFRGHEITKAENYRKLLLSMVKDVRVILVKFADRLHNMRTLEFVKPDKQRRIAQET
                     LEIYAPFAHRFGLGKVKWELEDLAFKYLNREAYEELARKLKAKRKDRESYIKKFAEPI
                     VKKLNEANLKIELSGRAKHLYSIYRKMVRRNKPFEEIYDLFAIRIILDTENRNDCYTT
                     LGIVNQIYLPVPDRFKDYISIPKTNNYQSIHTTVVGPLGRLVEVQIRTRQMHEVAEKG
                     VAAHWRYKENKTATDGELEAWVNWIRDIFENASRDDQKKELMESFKLNLYQDEIYVFT
                     PKGDLRRLPVGSTPVDFAFDIHSKVGFHCIGAKIDGKIVPLDTKLHSGDQVEIISSKN
                     QHPNKNWIKFVQTHKAKNEIRKWLNKEEQSIVDSGMEIWEKKIKKMKLSFTPDDVLKI
                     AHANKFDNSRQFFKAIAQSNINIDQVLTATTQKDDKEFEKELEFQKFAKISRSEIGGI
                     LVDGNKSGIMYTYAKCCNPIPGDPVIGYITIGEGIKIHRKSCVNLISLSANDSSKLVS
                     VQWPEAESSLFVAGISIRGKDRPGILNDISHSIVTYQNTNIKSLNINSSDSGFEGSVT
                     LYVNNLDHLNRIIERLKRVTGVYSVERFESN"
     gene            2789..3235
                     /gene="ruvX"
                     /locus_tag="VF870_06580"
     CDS             2789..3235
                     /gene="ruvX"
                     /locus_tag="VF870_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX9251889.1"
                     /translation="MPDIDETRIMGIDFGLKRIGIALSDSLKMFAYAHTTILNNSSSL
                     NELEKIVKEKNIIKFILGIPNEERQKKDSKTSIINDVKKFKESLEKKFNFEVELWDET
                     YTSAIAQQRILESVNKKVKRQNKDLLDMYSAAIILQEYLDSVKRNN"
     gene            3307..3588
                     /locus_tag="VF870_06585"
     CDS             3307..3588
                     /locus_tag="VF870_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX9251890.1"
                     /translation="MVYESLGVFETSSVSASLKALEGIIKEKQVNIVGKQLLGEGIVS
                     LFIKGDLGAIKRALAFGAEAIVSTNEFRSSHVIPLPHQNLFSIIGLKSK"
     gene            3591..4463
                     /locus_tag="VF870_06590"
     CDS             3591..4463
                     /locus_tag="VF870_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX9251891.1"
                     /translation="MSNSIGLIESKGIVALLEAADVILKNSPVKILGIHKLENGLVSL
                     AVIGNSDYVKAAIESGTEAGRRVGEIYASSVVENPGKEILEIFSELFPSDEISKNLPD
                     EAASLKSYSGEKNIEETSNVFASVTKQIKNESKREKSKPLQVTKVTPIQKNKKVKDKT
                     DHNDNTKLNESLENNAEDIEINNDDIDLTPESVKRLSTIERLRIEALGLESKKKDADE
                     IILPAKIKNDVEKKVKQTFKNSDVDFEAIDKMNVHKLRHYARAFPDFPIKGREISRAN
                     RDELVEWFRTMKND"
BASE COUNT         1676 a          613 c          870 g         1350 t
ORIGIN      
        1 tacaaatttt ggaaaagcaa aaaaacagaa tttattaatt ggctgccttt cgtagatgat
       61 gaaaaggaac tcggaattaa atatttacag agtgctgtaa agtattcagg atataattcg
      121 catctggcca tccactcttt aatttggatt tatatagaac aaaaagaatt tctaaaagca
      181 attgaggtag ctgagcttgc tttaaaggga catccgcaat ccagaatatt taaatgggga
      241 ttggcaagat cttatgaaaa tattgatccg gcaaagtctg ttttgttgta caaagaaatt
      301 ctttattcat atccaaaaga tcttaattct aacaagatta atgaagtgac actaaaacat
      361 ataattgcac agcagcttgt gaaaatcaat aaaagagatg aagctcttaa gatttgtaat
      421 gaaattttat ctataaatgg ttattcaaaa tatgaattag ataaattaag caatcgattg
      481 gatagagtga gagatttgaa aaaggaactg gaaaaacaat aaattatctc atttagtgaa
      541 tttctgtccc tttaactaat cattaaaaaa aactaaattg tttcggcatt aaaaattgta
      601 tcagtttaag ttaaataatg gcgatagata accctaaata tcaaaaaatg ttagacaacc
      661 ttatcaagat ttgtaaaaaa aatcttccgg ttgttaatga agcattaatt gctaaagctt
      721 tcgagttaag tcttgaagcg cataagaatg atatacgtgc ctctggtgag ccatatttta
      781 ctcatcctta tgaagttgcg atgatcgttg cagaggagtt tcctcttgat gatgttacaa
      841 ttgtaactgc gctgcttcac gatgttgttg aggatactga atactccatt gagtttatga
      901 ctcgtgagtt tggcaaagaa gtttctgaga taattgatgg tgttacaaaa ataagcggca
      961 tctttcgtgg acacgaaata acaaaggcag aaaattatcg gaaacttttg ctttcgatgg
     1021 ttaaggatgt acgtgtaatt cttgtaaagt ttgcagaccg cttgcacaat atgcgaactc
     1081 tggaatttgt taaacctgat aagcaaagaa gaattgcgca ggaaacttta gaaatttatg
     1141 ctccatttgc acatcgtttt ggattgggaa aagttaaatg ggagttagaa gatcttgcat
     1201 ttaaatatct taacagagaa gcttacgaag aacttgccag aaaacttaaa gcgaaaagaa
     1261 aagaccggga atcctatata aaaaaattcg ctgaaccgat agttaaaaaa cttaacgaag
     1321 ccaatctgaa aattgagctg agcggaagag ccaagcatct ttatagtatt tacagaaaga
     1381 tggtaaggcg taacaaaccg tttgaagaaa tttatgattt gttcgcaatc agaattattc
     1441 tggatacaga aaacagaaat gactgttata caacgcttgg aattgtaaat cagatttatt
     1501 tacctgtccc ggatagattt aaagattaca tctcaattcc taaaacaaat aattatcaat
     1561 ctattcatac gacagtagta ggacctttag gcagattagt tgaagttcaa atccgaacaa
     1621 gacagatgca cgaagttgca gaaaagggtg ttgcagcaca ttggcggtac aaagaaaata
     1681 aaacagcaac tgatggtgaa cttgaagcat gggtaaattg gattcgtgat atttttgaaa
     1741 atgcttcccg cgatgatcag aaaaaagagt tgatggagag ctttaaatta aatctttacc
     1801 aggatgaaat ttatgttttt actcctaaag gagatttaag aagattacct gtgggctcta
     1861 cgcctgtcga ttttgcattt gacattcata gtaaagttgg ttttcactgt attggagcaa
     1921 agatagatgg taagattgtt cctctagata caaaactgca cagcggtgat caggttgaaa
     1981 ttataagttc gaaaaatcaa catcctaaca agaactggat taagttcgtt cagacgcata
     2041 aagctaaaaa tgaaataaga aaatggctta acaaagaaga gcaatcgatt gtagatagcg
     2101 gaatggagat ctgggaaaag aaaataaaaa aaatgaaact ttcttttaca ccggatgatg
     2161 ttttaaaaat tgcacacgca aataaatttg ataattcaag acagttcttt aaagcgatag
     2221 cacaaagcaa tatcaatatt gatcaagtgc ttacagctac aacacaaaag gatgataaag
     2281 agtttgagaa agaattagag tttcagaaat ttgctaaaat ttccagaagc gaaattggag
     2341 gaattttagt tgatggaaat aaatcaggca ttatgtatac ttatgccaag tgctgtaatc
     2401 ctatccccgg tgatccggtg attggttata taactattgg ggaaggtatt aagatccatc
     2461 gtaaatcttg cgttaatctt atctcacttt ctgcaaatga ttcaagtaaa cttgtaagcg
     2521 tgcaatggcc tgaagcagaa agttcattgt ttgttgcagg catatcaatc cgtggaaaag
     2581 atagacctgg aattcttaat gatatttctc attcgatagt tacttaccag aatacaaata
     2641 ttaaatcatt gaatataaat tcgagtgatt ccggttttga aggaagcgtt actctttatg
     2701 ttaataacct cgatcatctg aatagaatta tcgaacgatt aaaaagggtt acaggtgttt
     2761 atagtgttga aagatttgaa agcaattaat gcctgatatc gacgaaacaa gaattatggg
     2821 gattgatttt ggattgaaaa ggattgggat agctctttca gattctctta agatgtttgc
     2881 ttatgctcat acaacaatat taaacaattc atcttcactc aatgaacttg aaaagatcgt
     2941 taaagaaaaa aatattatta agtttattct cggcatcccg aatgaggaaa gacaaaaaaa
     3001 agattctaaa acatcaatca ttaatgatgt taaaaaattt aaagagagtt tagaaaaaaa
     3061 atttaatttt gaagttgaac tttgggatga aacttataca tcggcaattg cgcaacagcg
     3121 tattttagaa tccgttaaca aaaaagtaaa aagacagaat aaggaccttc tggatatgta
     3181 ctcggcggct ataattttgc aggagtatct tgattctgta aaacgaaata actgaggtta
     3241 tttaattgac tttgctttgc atacagacta ttttttccta tcaaaatatg tatcgggagc
     3301 aaactaatgg tttatgaatc tctgggtgtt tttgaaacta gcagtgtttc tgcttcatta
     3361 aaagcattag aaggtattat taaggagaaa caggttaata ttgttgggaa acaattatta
     3421 ggtgagggaa ttgtatcctt gtttattaaa ggagatttgg gagcaatcaa acgtgctctt
     3481 gcttttggag cggaggcaat tgtttcaaca aatgagtttc gaagttcaca tgtgattccg
     3541 cttccgcatc aaaatttgtt ttcaattatt ggattaaaga gtaaataatt atgtcaaact
     3601 caattggatt aattgaatca aaagggatag tagctttatt agaagccgct gatgtaatcc
     3661 ttaaaaattc tcctgttaaa attttaggga ttcataaatt agaaaacggt ttagtttcat
     3721 tggcagttat cggcaattcg gattatgtaa aagctgcaat agaaagcgga acggaagcgg
     3781 gacgaagggt cggcgaaatt tatgcatctt ctgtagttga aaatcctggt aaagagatat
     3841 tagaaatatt tagtgaacta tttcccagtg atgaaatttc taaaaatcta cccgatgaag
     3901 ctgcttctct aaaaagttat tcaggagaaa agaatattga agaaacatca aatgtttttg
     3961 cttctgtgac aaagcagatt aaaaatgaat ctaaacgtga aaaatcaaaa ccattgcagg
     4021 ttacaaaagt tactccgatt caaaaaaata aaaaagtaaa agataaaaca gatcataacg
     4081 acaacactaa actgaatgaa tctcttgaga ataatgctga ggatattgaa atcaataatg
     4141 atgatatcga tttaactccg gaatcagtta agcgattaag cacgattgaa agattaagaa
     4201 tagaggcact tggtttagaa agcaaaaaaa aggatgcaga tgaaataatt ttaccggcaa
     4261 aaataaaaaa tgatgtagaa aaaaaagtaa aacagacttt taaaaacagt gatgttgatt
     4321 tcgaagctat tgataagatg aatgtacaca agctgcgtca ttatgctaga gcatttccgg
     4381 attttccaat taagggaaga gaaatttcac gggctaatcg tgatgagtta gttgaatggt
     4441 tcagaacaat gaagaatgat taattttaga aatgaaaaat aaaatttcca taatcatatt
     4501 atctgtact
//
LOCUS       DASWBY010000356         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_16343, whole genome shotgun sequence.
ACCESSION   DASWBY010000356 DASWBY010000000
VERSION     DASWBY010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16343"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VF870_06595"
     CDS             <1..988
                     /locus_tag="VF870_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251892.1"
                     /translation="LTSQQLSKYQAIIISANVIPSGIEQLKNYMQNGGGLILFPSSNP
                     DVHKLNEFYSQLGLALRASFVGKVNSVDLKIKFDKTDFNHPVFQNIFQNEEKKKYESP
                     ELNAYYKLTSAGNQIVSLIDGPPFLSEFKIGNGKTFVFDSSPVLSWSDFPIKSIFAPL
                     INKSVAYLSSKEREQNVFLAGEEVNINLKNTNASQIKIIKPDKTEEFINRTENSGRDY
                     LNYSNTSVAGAYKFYLGDNQIENISINTDPTESRTEYADQSVFEDYLKQINFNGKFTS
                     IDKDSNITEKIMQARFGSELWRYFLLIAIILALVEMAIARNTKKDLEGMDNK"
     gene            1091..2356
                     /gene="hflX"
                     /locus_tag="VF870_06600"
     CDS             1091..2356
                     /gene="hflX"
                     /locus_tag="VF870_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9251893.1"
                     /translation="MIEITKKVTERAMLVALDTKEYRKEVVEEHLAELEELANTAGAE
                     TVFKIIQSKSRMDSAYYIGKGKAEELAQLVELNDINIIIFDDDLTPVQVRNLSDLFKR
                     KVIDRSGLILDIFASRAKSKEAKTQVELAQLQYLLPRLTRAWTHLSKQYGGIGTKGPG
                     ETQIETDRRIIRTRISHLKEKLTEIESHRVIQSAGRKNSTRIAIAGYTNAGKSTLFNL
                     LTSSDVFAEDKLFATLDSTTRTLDFHETEKILISDTVGFIRKLPPQLVASFKSTLSEV
                     READIIFHVIDVSHPFYEDHLKVVEDTLKEFGSIDKKVVRIFNKVDLIKDKAKIDFIR
                     NTHKNSVMISAARGINISLLKSMLNDIVEDQFVEAKVTLKVSESKKVSQIHSLAEVLK
                     INYDEDKIKIHYKTSKQNSDKIKKIIHGS"
BASE COUNT          913 a          376 c          425 g          702 t
ORIGIN      
        1 gcttacatct caacagttaa gtaaatacca ggcaattatt atatcagcta atgttatccc
       61 ctctggaatt gagcagctaa aaaattatat gcagaatggc ggcggattga ttttattccc
      121 ttcatcaaat ccagatgttc ataaattaaa tgaattttat tctcagttgg gattagcatt
      181 aagagcatca ttcgttggca aagtaaacag tgtggatttg aaaattaaat ttgacaaaac
      241 agattttaat catccagttt ttcaaaatat ttttcagaat gaagaaaaga aaaaatatga
      301 atcacctgaa cttaatgctt actacaaatt aacatctgca ggcaatcaaa tagtttcttt
      361 gattgatggt ccgccatttt taagtgaatt taaaattggt aacggaaaaa catttgtttt
      421 tgactcttct cccgttttaa gctggagtga ttttcctatt aaaagtattt ttgctccttt
      481 gataaacaaa tcagttgcat atctttcatc aaaagaaaga gaacaaaatg tttttcttgc
      541 tggtgaagaa gtaaacatca atttgaagaa tacaaacgct tcgcaaataa aaattattaa
      601 gccggataaa actgaagagt ttataaatcg aactgaaaat tcaggaagag attacttaaa
      661 ttactccaat acaagtgtgg caggagctta taaattctat ttgggcgaca atcaaattga
      721 aaatatttca atcaatacag acccgactga atcaagaaca gaatatgctg atcaatcagt
      781 atttgaagat tatcttaagc aaataaactt caatggtaag tttacttcta ttgataaaga
      841 ttcaaacata accgaaaaaa ttatgcaggc aagatttggt tctgagttgt ggagatattt
      901 tttactcatt gcaattattt tagccttagt agaaatggca attgcaagaa atacaaaaaa
      961 agatttggaa ggaatggata acaagtaagt cattgcgagt cccaacaagt cgggacgaag
     1021 caatcttaac gataaacaga ttgtttcact cgaagactcg ttcgcaataa cacacaagag
     1081 aaaaaatatt atgatagaaa taacaaaaaa agtaactgaa cgagcgatgc tcgttgcact
     1141 tgatacaaaa gaatacagaa aagaagttgt tgaagagcac cttgcagaac ttgaggaact
     1201 tgcaaatact gcaggagcag aaactgtttt caagatcatt caaagtaaat ccagaatgga
     1261 ttctgcttac tacattggca agggtaaagc tgaagagctt gctcagcttg ttgaattgaa
     1321 cgacatcaac ataattattt ttgatgatga tttaactcct gtgcaggtaa gaaatctttc
     1381 tgatctattt aaaagaaaag tgattgacag aagcggcttg attctggata tttttgcctc
     1441 gcgagcgaaa agcaaagaag caaaaactca ggttgaacta gcacagcttc aatatctttt
     1501 accgcggtta acccgggcat ggacacactt atcaaaacag tacggcggaa taggtacgaa
     1561 aggtcccggc gaaacgcaga tcgaaactga cagaagaatt atccgtaccc gcatttcaca
     1621 tctaaaagaa aaactaactg agattgaatc gcatagagtt atacaaagtg ccggaagaaa
     1681 aaattcaact cgaattgcaa tagcaggcta cacaaacgca ggtaaatcaa ctctgtttaa
     1741 tttgcttaca agttcagatg tttttgctga agataaatta tttgcaacgc tcgattcaac
     1801 cacacgcaca cttgattttc acgaaacaga aaaaatattg atcagtgata cagttggatt
     1861 tattcgcaaa cttccacccc agcttgttgc atcatttaaa agtacactta gtgaagttcg
     1921 cgaggcagat attatttttc acgtgattga tgtttctcac ccgttttatg aagatcatct
     1981 taaagtggtt gaagatacct taaaagaatt tggaagcatc gataaaaaag ttgttagaat
     2041 atttaataaa gttgatttaa taaaagacaa agctaagatt gattttataa gaaacaccca
     2101 taaaaacagt gtaatgattt cggcagctcg tggaataaat atctctttac taaaatcaat
     2161 gctgaatgat attgttgagg atcagtttgt tgaagcaaag gtaacattaa aggtaagtga
     2221 atcaaaaaag gtttctcaaa ttcattctct tgctgaagta ctcaagatta attacgatga
     2281 agacaaaatt aaaattcatt acaagacaag taaacaaaat tcagataaaa ttaaaaagat
     2341 aattcatggc tcgtaaaaaa ttaatagtcg atggtaaatc actatcgtta gataaaatag
     2401 aatttttctt aaaaga
//
LOCUS       DASWBY010000357         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_727911, whole genome shotgun sequence.
ACCESSION   DASWBY010000357 DASWBY010000000
VERSION     DASWBY010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727911"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VF870_06605"
     CDS             <1..1070
                     /locus_tag="VF870_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator SirA"
                     /protein_id="HEX9251894.1"
                     /translation="EAAHRRDAEGRYASKTNENIPWQKIWRNLDWAVSHNLHTVDHLN
                     ERIAQGEFPHIVHESEALYEDDVELAANLIIERLDALRMVMISGPSSSGKTTTTIKLE
                     QKLIKKGFKFKALNVDHYFFDLEFHPKDEFGDYDFETPQALDLELINEHLVRLSNGEE
                     VMIPFYDFKTGTRTLNATPMKLAKDELLLIDSLHGLYPAFSKDISIEVKFKLYLEPLL
                     QMKGKDGRYIRWTDLRLIRRMLRDSVFRAYNPQQTLEHWHYVRGSELRNIIPYSNTAD
                     FVISSGMPYEVPIYANRMLKLFEEWKEKYKGDPLKVDALERSERVYNVLKTVTPVEDE
                     SSIPGDSVLREFIGGSTHQYH"
     gene            1246..2112
                     /locus_tag="VF870_06610"
     CDS             1246..2112
                     /locus_tag="VF870_06610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX9251895.1"
                     /translation="MKKFNHVLPILFSLILIFLFTDSIIAQNTPQKSRNSKRIYHKYF
                     DKSISSKSSAVNVEYRKDNRRNKDYSDYYESDRRVISEREIKRKNSFNNYDNEDSENY
                     FRIDKSKNSLWDGKHWEESDFPLNIFVQESSSRYYKSSYKDYVKYAMDVWRKADDRIQ
                     YKFVNSARDADIEIIFVENLGDKYEENYLGLTDYDTNRNKEIEFSKIQISLIKNGNEK
                     VSAGEIKATIVHEFGHAFGLGHSDNENDLMYSYISPKHTPEMTYDELSKGDKLAIKDV
                     INLSFDKKYVWK"
     gene            2245..>2526
                     /locus_tag="VF870_06615"
     CDS             2245..>2526
                     /locus_tag="VF870_06615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX9251896.1"
                     /translation="MARLLILGAGIAGQTAALHAKNKLGNKHEVVVVSPNSKWNWIPS
                     NIWVGVGMMKPEQVTFQLAPVYKKTGITFYQAKAVSIHPEGDQSSQSPFV"
BASE COUNT          906 a          405 c          460 g          755 t
ORIGIN      
        1 aagaggccgc acacagaagg gacgctgaag gcagatatgc cagcaagact aatgaaaata
       61 ttccatggca aaaaatctgg cgtaatcttg attgggcggt ttcacataat cttcacactg
      121 tcgatcattt aaatgagcga attgcacagg gagagtttcc acatattgtt catgaatctg
      181 aagccttata tgaagatgat gttgaacttg ctgcaaattt aataattgaa agacttgatg
      241 cgcttagaat ggttatgatc agcggcccat cttcatcagg aaaaacaaca acaacaatta
      301 agcttgaaca gaaactgata aaaaaaggat ttaagttcaa agcacttaat gttgatcatt
      361 acttttttga tttagagttt catcccaaag atgaatttgg tgattatgat tttgaaactc
      421 cgcaggcttt agatctagaa ttgattaacg aacatcttgt aagattaagc aacggcgaag
      481 aagtgatgat tccattttac gactttaaaa ccggaacgcg aacactaaac gctaccccaa
      541 tgaaacttgc aaaggatgaa ctcttattga ttgacagctt acacggacta tatccggcat
      601 tcagtaaaga catttctatt gaggtaaagt ttaaattata tcttgaacca ttgcttcaaa
      661 tgaaaggtaa agatggaagg tacattcgct ggacggattt aagattgata agaagaatgt
      721 tgcgtgattc agttttccgt gcttacaatc cgcagcaaac tttagaacat tggcattatg
      781 tacgcggcag tgagctaaga aatataattc catattcaaa cacagcagat tttgtaataa
      841 gcagcggtat gccttatgaa gttccaatct atgcaaacag aatgttaaaa ctttttgagg
      901 agtggaaaga aaaatataaa ggcgatccgc ttaaagttga tgcgctggag agatctgaac
      961 gggtttataa tgtgttaaag accgtaactc cggttgagga cgaatcatct attcccgggg
     1021 acagcgtgtt acgagaattt ataggtggca gtacccatca ataccattaa aacttgatta
     1081 tcattttaga cattccccag ggaatagatt ctaaataaga cattagctta gaataaatat
     1141 cagtttttat ttcgttttgt aactaatacc aatggaatag aatttgataa atctaatggg
     1201 ttatgtttaa actatttcaa ttctattttt agagacgatt gtttcatgaa aaaattcaat
     1261 cacgttttac caatcctgtt ttctttaatt ctaatatttc tattcaccga ttccatcatc
     1321 gctcaaaaca ctccccaaaa gagcaggaat tctaaaagaa tctatcataa atattttgat
     1381 aaaagcattt catcaaaaag ttctgcagta aatgttgagt atcgtaaaga taaccgccgc
     1441 aataaagatt attcagatta ttatgaatcc gacagaagag taatttcaga acgcgaaatc
     1501 aaaagaaaaa atagttttaa taattatgat aatgaagatt cagaaaatta ttttagaatc
     1561 gataaatcaa aaaatagttt gtgggatggt aaacactggg aagaatcaga ttttccttta
     1621 aacatttttg ttcaggaatc ttcttcgcgc tattacaaat catcgtataa ggattatgta
     1681 aaatatgcaa tggatgtttg gagaaaagct gatgatagaa ttcaatataa gtttgttaac
     1741 tctgcccgtg acgcagatat tgagattata tttgttgaaa atcttggcga caagtatgaa
     1801 gaaaattatt tgggtttaac tgattatgac actaacagaa acaaggaaat agaattttca
     1861 aaaattcaaa tcagcttaat aaaaaatggc aacgagaaag tttctgcggg agaaattaaa
     1921 gcaactatcg ttcacgagtt tggacatgct tttggacttg gacatagtga caatgaaaac
     1981 gacttaatgt attcatacat ttctccaaaa cacacgccgg aaatgacgta tgatgaatta
     2041 tcgaaaggcg ataaacttgc aattaaagat gtaatcaatc taagctttga taaaaaatat
     2101 gtctggaaat aatgtgccgg tttagattca ttctcaccca aaatcaacaa aaataattta
     2161 caaacctaat attttatcaa agtattattt aagttactgc cataattgtt tttgtgttcc
     2221 taaaaattaa tcttaggagt aacaatggca cgcttactta tacttggtgc aggaatagcc
     2281 ggacaaacag ctgcactcca cgcaaagaac aaactcggaa ataaacatga agttgtagtt
     2341 gtttcaccta acagcaaatg gaattggatt ccatcaaaca tttgggttgg cgttggcatg
     2401 atgaaacctg aacaagttac ttttcaactt gctcctgttt ataaaaaaac aggaatcaca
     2461 ttttatcagg ctaaagctgt aagcatacat ccggaaggtg accaatcttc acaatctcct
     2521 tttgtt
//
LOCUS       DASWBY010000358         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_239244, whole genome shotgun sequence.
ACCESSION   DASWBY010000358 DASWBY010000000
VERSION     DASWBY010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239244"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VF870_06620"
     CDS             <1..313
                     /locus_tag="VF870_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9251897.1"
                     /translation="FINAILSHADYSGVPRDTSLKKVLFACDEIAGFITAVTYVRPSK
                     SIDEVEVSSVKKKMKDKAFARAVNRDDMLNGAEGIGIALDEHIDFCIKAMRKNKDLLG
                     L"
     gene            310..759
                     /locus_tag="VF870_06625"
     CDS             310..759
                     /locus_tag="VF870_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX9251898.1"
                     /translation="MKIGEMVENFQLNDQFNNKFDLYENLDEDVLLVFYPKDNSLVCT
                     RQLVDYNSNLDELKKIGIKIVGINIDPVQSHNSFCTNNRIDFPVLSDPDKKVSKFFEA
                     LNPIGQNKRKLVLVGKDKLVKYQRSVLSVFFLDSKELLKDLREKQLL"
     gene            885..>3145
                     /locus_tag="VF870_06630"
     CDS             885..>3145
                     /locus_tag="VF870_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEX9251899.1"
                     /translation="MTSKIFIILLLISTPLLFSQRDIKVLSSDFNSITIEYSPKIIDS
                     SYVTVDGKTFRKVEISSGILKNYNDWGMPSIIERNLNVGVPSEVGNTITVLSSVYKEL
                     SGQILPVPYPVQDTFSVSFDYKQNSDYSGFKSIEDIVTFGEYGLLRDVPMQTINISPI
                     KFDPASNKIKLYTKIIFRIDFSNQGIISSKPADDLIDGILINYDVAKYWNKRYSDKSL
                     NKVAVENSVLASGKWVKFEAPEEGIYKISKANLALFGIDANAVDPRTIKIYNNGGKVY
                     PENITSARPIDLVENAILVVGQEDGKFDDADYILFYGRGSSFWDFDTDGSTIKRFKNP
                     YSTKNYFWITSGSTNGKRIIEKPGLTTTPALIQTSSSAYADWEIDKINLGKTGRQFFG
                     DDFSSSVTSRTYTNSLNGRINGTPINYNLRFAIGSPSGLNLTVTENGNQLFQRNLAGY
                     GSTSYTAGIANIWSFVFNGNLPDNRSVLNFSVIPSGVSAVGYLDYFTIQYEKELKAFG
                     DNLLFYSSPSGGLIEYQLNGFTSSNIRVFDITDFSNVRSVSNYVMQSGSEVRFQFDES
                     LTQRTKYYAVGSDAFKTPSNPVEVQNSNLRGEEPGAKFIIVTNKAFKEAANNLKNYRE
                     NQALVTISTYVADIDQIYNEFSGGTMDPTALRDYLKYAFDNWTIKPQYVLLFGKGTYD
                     YQNIEKYGDNFVVTWQSDESLSLLNSYTTDDFFGRLSGDDNTVDIALGRITCANPNEA
                     NAMVNKIIDYELN"
BASE COUNT         1096 a          461 c          570 g         1018 t
ORIGIN      
        1 atttataaat gcaattttat ctcatgcaga ttatagcggg gttccaagag atacttctct
       61 aaaaaaagtt ttatttgcat gcgatgaaat agctggattt ataactgcag taacttatgt
      121 tcgaccctca aaatccattg acgaggttga agtaagttct gttaaaaaga aaatgaaaga
      181 taaagcattt gcccgggcgg ttaatcgtga tgatatgtta aatggtgctg agggaattgg
      241 tattgcattg gatgagcaca tcgatttttg tatcaaagcc atgagaaaaa ataaagatct
      301 gcttggatta tgaaaattgg ggaaatggtt gaaaattttc aactgaatga tcaattcaat
      361 aataagtttg atctttatga aaatttggat gaggatgttt tactagtttt ttatcccaag
      421 gataattcac tggtatgtac cagacaatta gttgattata actcaaacct ggatgaacta
      481 aaaaaaattg gaataaagat agtcgggatt aatattgacc ctgttcaatc acataattca
      541 ttttgtacga acaatagaat tgattttccc gtattaagtg atccagataa aaaagtaagt
      601 aaattttttg aggcactaaa tccgatagga caaaataaaa gaaaacttgt tttagtgggt
      661 aaagataaac ttgtaaaata tcaaagatct gtactgtctg tgttttttct cgattcaaag
      721 gagttattaa aagatctcag ggaaaagcaa cttttataaa gacttgacta tttgataaat
      781 agatgccatt tttgttacaa aattaggaga aatttttaga gatacttgct acattagtaa
      841 atccgaatta aatagaaaat caattaaaaa ttagtttgat ttaaatgaca agcaagatct
      901 ttataatcct attactcatc agtacgcctc ttctgttttc tcagcgtgat attaaagtat
      961 tgtcatcgga tttcaattct ataaccatag agtattcccc aaaaattatt gattcttcct
     1021 atgtaacagt tgacggaaaa acgttccgaa aagttgaaat atcatcaggt attctcaaaa
     1081 actataatga ttggggaatg ccttcaatta tagaaagaaa tcttaatgtc ggtgttccat
     1141 cagaggttgg taatacgata acagttcttt cttctgttta taaagaacta tccggtcaaa
     1201 ttcttccggt tccatacccg gttcaggata cattttcagt ttcgtttgac tacaaacaaa
     1261 attctgatta ttcaggattt aaatcaattg aagatattgt aacttttgga gaatatgggt
     1321 tattaagaga tgtcccaatg cagactataa atatttctcc aattaaattt gatcctgctt
     1381 ctaataagat aaaactttac acaaaaatta ttttcagaat tgattttagt aaccagggaa
     1441 taatatcctc aaaaccggct gatgatctaa ttgatggtat tcttatcaat tatgatgttg
     1501 ccaagtattg gaataaacga tactctgata aaagtctaaa taaagttgct gtagagaaca
     1561 gtgtccttgc aagtggtaaa tgggtaaaat ttgaagcacc tgaagagggc atctacaaaa
     1621 tatcaaaagc aaatttggct ttatttggta ttgatgcaaa cgcagttgat ccaagaacaa
     1681 taaaaattta taacaacggc ggaaaagttt atcccgaaaa tataacctca gcaagaccaa
     1741 ttgatcttgt tgagaatgct attctggtag ttggacagga agatggaaag tttgatgatg
     1801 cggactatat cttattttac ggaagaggca gcagcttttg ggattttgat actgacggat
     1861 caacaattaa aagatttaaa aatccatatt caacaaaaaa ttatttctgg attacttcgg
     1921 gcagtacaaa tggaaaacga atcattgaaa aacctggatt aacaacaaca cctgctttaa
     1981 ttcaaactag ttcttctgct tatgcagatt gggagatcga taaaattaat ttaggtaaga
     2041 cagggcgcca gttttttggc gatgattttt cttcatcggt tacatcgaga acttatacca
     2101 atagtttgaa tggaagaatt aatggaacac cgattaatta caacttaaga ttcgcaatcg
     2161 gatcgccatc tggtttaaat ttgactgtta cagaaaatgg taatcaatta tttcagcgta
     2221 atctagcagg ctatggttct acatcgtaca ctgctggaat agctaatatt tggtcttttg
     2281 ttttcaatgg aaatttgccc gataacagaa gtgtattaaa ctttagtgtg ataccatctg
     2341 gagtttcagc tgtaggttat ctggattatt ttaccattca atatgaaaaa gaattaaaag
     2401 catttggtga taatttacta ttctattcta gcccttcagg aggattgatt gaatatcagt
     2461 taaatggatt tacttcatca aatatcagag tatttgatat tactgatttt tcaaatgtaa
     2521 gatctgtttc taactatgtt atgcaaagcg gcagtgaagt ccgttttcaa tttgatgaaa
     2581 gtctaaccca gagaactaaa tattatgcgg ttggaagtga tgcatttaaa actccatcaa
     2641 atcctgttga ggttcaaaac tccaatctaa gaggtgaaga gccaggtgca aaatttatta
     2701 tagttactaa taaagctttc aaagaagcag caaataatct taaaaattat agggaaaacc
     2761 aggctctggt tacaatctcc acctacgttg cagatattga tcagatttat aatgagttct
     2821 caggcggtac gatggacccg acagctttga gagattactt aaaatatgca tttgataatt
     2881 ggacaataaa accgcaatat gttttgctct ttggcaaagg tacatatgac tatcagaata
     2941 ttgaaaaata tggcgataac ttcgttgtca cctggcaatc tgatgaatca ttatcattat
     3001 taaactcata taccaccgat gacttttttg gacgtttgtc cggtgatgat aatactgttg
     3061 atatcgcttt aggtagaatt acatgtgcaa atcccaatga agcaaacgcg atggttaaca
     3121 agattatcga ttacgaatta aatca
//
LOCUS       DASWBY010000359         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_16435, whole genome shotgun sequence.
ACCESSION   DASWBY010000359 DASWBY010000000
VERSION     DASWBY010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16435"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VF870_06635"
     CDS             complement(<1..529)
                     /locus_tag="VF870_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9251900.1"
                     /translation="MKKQFSIFLFIVSLVSTSQIIAQEVDIVPYLKMIEQGKVEEVKG
                     ILIDLKTDYPKSSNLIFLEGVLTENGQDAVALYQKLVEEYPKSTYADAAIYRIYSYYF
                     ALGLYNTADKNLDKLKKDYPESPYIKMAAANVVKNDVEETTQPLPVENHNVEKEFRYT
                     VQAGAFTNVDNANSLK"
     gene            complement(574..1935)
                     /gene="miaB"
                     /locus_tag="VF870_06640"
     CDS             complement(574..1935)
                     /gene="miaB"
                     /locus_tag="VF870_06640"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX9251901.1"
                     /translation="MQKNNVYIETYGCQMNLADTEIVLGILKKQGYDVTDKAEDADVV
                     LLNTCSIRDNAEQRIYGRIGNLKTLKVNKPELVLGILGCMAERLRKDLVEDKKAVDLV
                     VGPDEYRRLPEYIDIALNGDKGIGVKLSRTETYDDIEPHREDGLSAWISVMRGCDKFC
                     TFCVVPFTRGRERSRSLQSIITELENLSGRGFKDVTLLGQNVNSYLDETSAASGDFAD
                     LLSAAAKVDRSMRIRFSTSHPQDISDKLLYTIAEHPNLCNYIHLPIQSGSNRILELMN
                     RTYTVEHYLNLIEKAKKIIPGVSFSTDIISGFPTETWEDHFATLDVLRQVRYDGAYMF
                     KYSPREGTKAHRMNDDVPEDVKSKRLQEIIDVQQQISFEKNQALISTEEIILVEGFSK
                     KSNDFIAGRTDTNKTVIVPVNDEIKTSDYIKVKINRATSGTLFGDYLSHIQPEKEGIA
                     LTA"
     gene            complement(1963..2124)
                     /locus_tag="VF870_06645"
     CDS             complement(1963..2124)
                     /locus_tag="VF870_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251902.1"
                     /translation="MFGFVKTVEKQKIIDNNDLLLEKKRKEKRFMILFAIIALSILNI
                     SLYFLTKIN"
BASE COUNT          691 a          416 c          334 g          767 t
ORIGIN      
        1 tttttaatga atttgcatta tcaacatttg taaaggcacc cgcttgtaca gtatagcgaa
       61 attctttttc cacattgtgg ttttcaactg gtagtggttg agttgtttct tcaacatcat
      121 tcttaacaac atttgcagct gccattttta tataaggcga ttccggataa tctttcttta
      181 acttatcaag atttttatcc gcggtattat ataagcctaa agcaaaatag taagaataga
      241 tccgataaat cgctgcatca gcataagtgc ttttaggata ttcttcaacc aacttttgat
      301 acagtgccac ggcatcctgt ccgttttctg taagaacacc ttctaagaaa ataagatttg
      361 aacttttggg ataatctgtt tttaaatcga tcagtatgcc ttttacttct tcaacttttc
      421 cctgctctat catcttcaga tatggaacaa tatcaacttc ctgggcaatg atttgtgaag
      481 ttgaaaccaa tgagacaata aaaagaaata tcgaaaattg ttttttcata aatctgaaac
      541 agactaaaaa ttgtttaaca caaattagtt tgtttatgct gtaagcgcaa taccttcttt
      601 ttcaggctgg atatgagata gataatctcc gaataatgtg ccggatgtag ctctgtttat
      661 tttaacttta atgtaatcgc tggtttttat ttcatcattt accggaacga ttactgtttt
      721 atttgtgtct gttctgccgg caataaaatc attagacttt ttactaaatc cttcaactaa
      781 tataatttct tctgtactaa ttagtgcttg atttttttca aaagaaattt gctgctgcac
      841 atcgattatc tcttgcaatc tttttgattt aacatcttca ggcacatcat cattcattct
      901 gtgtgctttt gttccttcac gcggagaata tttaaacata tatgcaccat cgtatctaac
      961 ctgtctaaga acgtccagag ttgcaaaatg atcttcccac gtttctgttg gaaatcctga
     1021 aataatgtcc gtagaaaaac ttactcccgg aattattttt tttgctttct caatcaaatt
     1081 aagataatgc tcaacagtat aagtacgatt catcaattca agaatacggt ttgatcccga
     1141 ctgaattgga agatgaatat agttgcatag atttggatgt tctgcaatcg tgtataaaag
     1201 tttatcagaa atatcctgag gatgcgatgt gctaaatctg attctcatgc ttcgatcaac
     1261 ttttgcagca gcagaaagca gatctgcaaa atctccactg gcagcggatg tttcatctaa
     1321 atatgagtta acattctggc caagcaatgt tacatcttta aatccacgac cagagagatt
     1381 ttctaactca gtgataattg actgaagtga tctgctgcgt tcccttccgc gtgtgaatgg
     1441 aacaacacaa aaagtacaga acttatcaca accgcgcata acagaaatcc atgcagaaag
     1501 tccatcttca cgatgaggtt caatatcatc ataagtttct gttcgggaaa gtttaacacc
     1561 aataccttta tcaccattta gtgcaatatc aatgtattca ggcaatcttc tgtactcatc
     1621 cggaccaaca actaaatcaa cagctttttt atcttcaaca agatcttttc ttaatcgctc
     1681 agccatgcag ccaagaattc caagcaccag ttcaggtttg ttaactttaa gggtcttaag
     1741 atttccaatt ctgccataaa ttctttgttc agcattatcc cgaatgctgc aggtattcag
     1801 cagcaccaca tctgcatctt ctgctttatc tgtaacatca taaccctgct tctttaaaat
     1861 accaagtaca atctcggtat cagcaagatt catctgacag ccatacgttt ctatataaac
     1921 attatttttt tgcattacca aaaattaata ctgagtgaaa ggttaattga tttttgtaag
     1981 gaagtaaaga gaaatattta atattgataa agcaatgatg gcaaagagaa tcataaatct
     2041 tttttctttc ctttttttct ctaaaagcag atcattatta tcaattatct tttgtttctc
     2101 tacagtttta acgaatccga acatttaatc ccgcaaatat tttgtgtaat tattacttaa
     2161 taataaggat aatttcaaag aaaatacagg taatacttta tcaaaaaa
//
LOCUS       DASWBY010000360         1864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_314672, whole genome shotgun sequence.
ACCESSION   DASWBY010000360 DASWBY010000000
VERSION     DASWBY010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314672"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VF870_06650"
     CDS             complement(<1..544)
                     /locus_tag="VF870_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251903.1"
                     /translation="MQSVTIEDLKKVIALRDLSDDTLQWLLKHSESMEYEDGEVVAKT
                     GDPVDWMFFIIEGSLDFYMDVNGRLIYFYHFANDDESGGVTGLLPYSRMKAYPGNSIA
                     VDKLQGLRLHKKYFQELEQLDPAFIQRCIGYMTERAKSFATKQLQLEKVNALGNLAAG
                     IAHELNNPASAINRISFELTN"
     gene            1384..1692
                     /locus_tag="VF870_06655"
     CDS             1384..1692
                     /locus_tag="VF870_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251904.1"
                     /translation="MKWFYIKFTNEELVAKRDTKFINQFLKLLQYLDHPENLGLYSLR
                     TKMNEGLVYYASTPPEVSYKLRELLIHYRSKEVSRPNLNVLRLELGKSEILEDQLIFM
                     "
BASE COUNT          644 a          315 c          275 g          630 t
ORIGIN      
        1 ggtttgttaa ctcaaaggaa attctattaa tcgcagaagc aggattattc aattcatgtg
       61 caatgccagc ggcgagattt ccaagagcat ttactttctc aagctgcagt tgctttgtag
      121 cgaatgattt agcacgttct gtcatatatc caatgcaccg ctgaataaat gccggatcaa
      181 gctgttccag ctcctgaaaa tattttttat gcaacctcaa tccttgaagt ttatcaacag
      241 caattgaatt accaggataa gctttcattc ttgaatatgg cagcaatccg gttacaccac
      301 cagattcatc atcatttgca aagtgataaa aataaatcag tctgccgttc acatccatat
      361 aaaaatctag actaccttca attataaaaa acatccaatc aacaggatca ccggttttcg
      421 ccacaacttc accatcttca tattccatac tttcagaatg cttcagaagc cattgcaggg
      481 tgtcatcaga taaatcgcga agtgcgataa cttttttcaa atcttcaatt gttacagatt
      541 gcatacttgt aaaaagtgta taagtatata aatagaagta atcattatgt tattactata
      601 attttttctc tgattaagat aattcggatt attctaatcg aagattgtta aaaaactaat
      661 aataagtcaa gtgatatact taatttattt tcaggaatat caaacttctt ctttattctc
      721 cgcatttcaa gaactgattc agtatcagtc ttccgctcca cctcaatata tcttttagtc
      781 aacttcatcg aagtaaactt aatatctaaa aaatcaggat acttttcatc aaaaatccgt
      841 cagcaagccg agtaatcagg acatcacgtt ggttttcttc ttttaagatt gaagtctgtt
      901 aaaaattgga gagatacgat gacacagttt ttgcttaagt gctgtaaaga gttttaacta
      961 tccattaata gaatcattac aatgtacttt aaatcagtcg gcaattatat tcattttgta
     1021 aaaactaaaa agaatagaaa ttgattgact ttgaaaatag tattaaatac atttgacaag
     1081 agttacgtaa agttttcagc gtaaactcct tgtagattta tttaagatcc ccgatagcct
     1141 cccacatcgg ggatttttat ttctcaatat tgaaataaaa aaataaattt ttattatatg
     1201 taataactga agctgaccaa atttgtcgtt tgccggatta atacttgaaa aaattaaatt
     1261 attaaatact acacatttgg catcgtttta gtgaggctat taaaatattg aatgtgatgt
     1321 aaatgtgttg tattggcctc tgatttactt gaattaattg attagatata gacttggtgt
     1381 tatatgaaat ggttttatat taaatttact aatgaagagc tcgtagcaaa aagagataca
     1441 aaattcatca atcaattcct caaactcctc cagtatctcg accatcctga gaacttagga
     1501 ttatattctt taaggactaa aatgaatgaa ggacttgtat actatgcctc aactccaccg
     1561 gaagtatcat ataaattgag ggagctttta atacactatc gatctaaaga agtttcgaga
     1621 ccaaacttaa atgttttaag actcgagtta gggaaaagtg aaatacttga agatcaactt
     1681 atatttatgt gatttttata gaatccgtct attaacattt tttatagttg catactgtct
     1741 tcaaaggatc attcacataa aaactaaatt atcggtaaag taaaataaaa attagagccg
     1801 tttttctccg aacttttcaa tccaatctca cccttgtgca aattaattaa ttgcttcgca
     1861 attg
//
LOCUS       DASWBY010000361         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_314874, whole genome shotgun sequence.
ACCESSION   DASWBY010000361 DASWBY010000000
VERSION     DASWBY010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314874"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2110
                     /locus_tag="VF870_06660"
     CDS             <1..>2110
                     /locus_tag="VF870_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9251905.1"
                     /translation="LLDVKNFTERFNKSFSGIKTILINGFDEFTQPEINIINRAAEIE
                     GIELYVMFDYYRYNPALFSHLDSCYEKFKARGFCEVEDISPIQFNDYQKRIREKLFLL
                     NENDTPKASEIEIVKIIAQSPEEEIRLIAKEIKLLITKKNVDVDSITIAFNLISDHSA
                     IIRDVFNEYGIPFNLTDRYALNESQPVIALINFLEIQENNFYYKNIFRALTGRWIKID
                     AVDLSNLLRVSSNLKIVSGYKNWIDSISRTIDEINYNNDDEDNRFLPIEFYIKAKDDV
                     EAIFEMLKPFREKKTIYEFKDELRTLIYTLSLPQRIVNDHEDYIEKNVKAVSVFLETL
                     DELFDLLADENGTDKKFPLSYFLSQIKNALQFTRYNIKERHASGVLITSVNEIRGLNF
                     DYVFIGGLVDGEFPTRYQPEIFLSGSYKKDEYRHILEDRYHFYQTLCCAKKRLYLSYA
                     MKDEKKEFTPSTFVNDLSRLFSVKEKSSKDYSALIYSRSELMKYVASLDLEKDKDNIK
                     TLGIDTEKLKTDLSVDKLRLENPFGETEFTGNISINVSEEAKTKLAEQAEKQYSASQL
                     EEYAKCPFQYFLKRILQLETIEEPTEELESFELGSIIHSILYEFYSSIKEKKIILSLC
                     DDVTFRQAEKLIFSIAEKKIEKLRLNSTMIFFEREKILGIAGNKKKSILYKFLEEERN
                     NSEGFIPQYFELGFGKFNNSK"
BASE COUNT          789 a          293 c          363 g          665 t
ORIGIN      
        1 ttactcgatg taaaaaattt tactgaaaga tttaataaat cgttcagcgg aataaaaaca
       61 atcttaatta atgggtttga tgagtttacc cagcctgaga ttaacataat taatcgagca
      121 gcagaaattg aaggcatcga actttatgta atgtttgatt attaccgata caatcctgca
      181 ttgttttcgc accttgatag ctgttatgaa aaatttaaag ccaggggttt ttgtgaagtt
      241 gaagatatat ctccaattca gtttaatgat tatcagaaaa gaatacgtga gaaattattt
      301 ttattgaatg aaaatgatac gccaaaagcg agtgaaattg aaatagtgaa aatcattgca
      361 cagtctcccg aagaagaaat aagacttatt gccaaagaaa taaaattact aattacaaaa
      421 aagaacgttg atgttgattc aataactata gcttttaatt taatatcaga tcactcggca
      481 attatacgcg atgtttttaa tgaatatgga attccattta atcttacaga cagatatgcg
      541 cttaatgaat cgcagccggt aattgctttg ataaattttc tggaaattca ggaaaataac
      601 ttttactata aaaatatttt ccgtgcatta acaggaagat ggataaagat agacgcagtc
      661 gatctttcaa atctgcttcg tgtttcttca aatcttaaaa tagtttccgg ctacaagaac
      721 tggattgatt caatatccag aactattgat gaaataaatt ataataatga tgacgaagat
      781 aacagatttt taccaattga attttatatc aaggccaaag atgatgttga agcaattttt
      841 gaaatgctta aaccttttag ggaaaagaaa accatttatg agtttaagga cgaactaaga
      901 acactaatct acactttaag tctgcctcag cggatagtta atgatcatga agattatata
      961 gaaaaaaatg ttaaagctgt ttcagtattc ctggaaacac ttgatgaatt atttgaccta
     1021 cttgctgatg aaaatggaac tgataaaaaa ttccctttaa gctatttttt atcacaaatt
     1081 aaaaacgctc tgcagtttac ccgctacaac attaaagaaa gacacgctag cggagtgctt
     1141 ataacatcgg ttaacgaaat ccgtggatta aactttgatt atgtctttat cggcggtttg
     1201 gttgatggtg aattcccaac tcgttatcag ccggaaatat ttttatccgg ttcatataag
     1261 aaagatgaat acagacatat acttgaggat cgttatcatt tttatcaaac tttatgttgc
     1321 gcaaagaaaa gattgtactt gtcttatgcg atgaaagacg aaaaaaaaga atttactcct
     1381 tcaacatttg taaatgattt gtcaagactt tttagtgtta aggaaaaatc atctaaagat
     1441 tattcagctc ttatttatag tagatctgag ttaatgaagt atgttgcctc tctggattta
     1501 gaaaaggata aggataatat taagacactt ggtattgata ctgaaaaact taagactgat
     1561 ttaagtgttg ataagttaag attagaaaat ccatttggtg aaactgaatt tacaggtaac
     1621 atatccatta atgtttctga agaagcaaaa acaaaacttg ctgagcaggc agaaaaacaa
     1681 tactcagcat cccagcttga agaatatgca aagtgtccgt tccaatattt cctgaaaaga
     1741 attttacaac tcgaaacaat cgaggaaccc actgaagaat tagaatcatt tgaactcgga
     1801 agtataattc actcaatctt atatgagttt tacagctcaa taaaagaaaa gaaaataatc
     1861 ctttcactat gcgatgatgt tacatttagg caggctgaaa aattgatttt cagtattgca
     1921 gagaagaaga tcgagaaatt aagacttaat tccacgatga tattttttga gagagaaaaa
     1981 attcttggta ttgcaggaaa caagaaaaaa tctattttat ataaattcct tgaagaggaa
     2041 agaaataatt cagaaggatt tattcctcag tattttgaat tgggatttgg aaagtttaat
     2101 aattcaaaag
//
LOCUS       DASWBY010000362         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_239773, whole genome shotgun sequence.
ACCESSION   DASWBY010000362 DASWBY010000000
VERSION     DASWBY010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239773"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..606)
                     /locus_tag="VF870_06665"
     CDS             complement(340..606)
                     /locus_tag="VF870_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251906.1"
                     /translation="MIALQTHISYNFNPIMWAAIDLTYYTGGQSSINDIYNDDRQNNS
                     RIGATFNFPISKLSAIKIAYSTGAIIRFGSNFSTISAAWQMAFL"
     gene            complement(774..2480)
                     /locus_tag="VF870_06670"
     CDS             complement(774..2480)
                     /locus_tag="VF870_06670"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX9251907.1"
                     /translation="MNYISISRNLLFIFSLLILFSCKPSPEKEGMQSNSNKPNILLIV
                     ADDLGFSDIEPFGSKIIKTPEIDKLARGGMLFANFHVLPTCSPTRSALLTGNDNHVAG
                     LGIMSELDYPQLRELNLPGYAGALSKQVVTIPELLRTVGYNTYMTGKWHLGEEQGENP
                     FDRGFEETFILGTGGGSHFNDERALSPLQHMDYTRNGKEVELPTDFYSTKNYTDSLLH
                     FIDMHKEDKKPFFAYLAYTAPHDPLHAPEEYIKKYKGKFDDGWDALRLSRLENLKALG
                     LMPEQLNNFAPNPMIPKWETLSKKQQQAFARDMEVYAAMVEYVDMSIGRVINYLKENG
                     MYDNTMIFFFSDNGANGAVASTYPGNEDGKYLSTFNNEMDNRGLKGSYIAMGPGWAQA
                     VSSPLRLFKTFTTEGGIRAPLIIKTPGTNNTPGKWNYSFVHVTDIMPTLLELTGATYP
                     EEFNGHKIHGLIGKSILPILTGKVQSVHDNTGMGYELFEMKAYIKGKWKILRLPQPFG
                     AGYWQLYNLEVDPGEINDVSDQHPEIKEELIEEWMHYSKTNEVFDHKGHYDSLYIRSY
                     GQ"
     gene            complement(2596..3033)
                     /locus_tag="VF870_06675"
     CDS             complement(2596..3033)
                     /locus_tag="VF870_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251908.1"
                     /translation="MKKNKSIFYAAILIIAALIASYFIFFHSSITETVEESETISTSI
                     DKNIIAGSWIRTDAEYQIKISDVSENGVMSAQYFNPRPINVSKAEWAYTEGFLKVYIE
                     LRDENYPGSNYNLTYIPERDLLVGDYFQAVEGLTFYVEFRRNK"
BASE COUNT         1061 a          590 c          549 g         1170 t
ORIGIN      
        1 ttcctttttt taatacaaaa tattttgtgg cttaaactta ttagaaatat ttgaaacatt
       61 gcttcactaa tttcttagtc tgctttattt catttagaag tttaaataac ccagtgttct
      121 aaatcggtat tattctgaaa ccgaacattt atcactgatg ttattgtatt ggcaagttac
      181 attgctaatt ctttagctga ctgcgctata gaatttattt gcaatagaat tagaagtgca
      241 tagattaatt taatcattct ttatttacaa cctttcttag tgaattacta ttaatattaa
      301 tttgaattta aaaacaatta agtgtttaca caagtggttt tataaaaacg ccatttgcca
      361 tgcggctgaa atagttgaaa agttagaacc gaatcggata attgcacctg tactatatgc
      421 aatttttatt gcgctcagtt tactaatcgg aaagttaaat gttgcaccaa ttcgcgaatt
      481 gttttgcctg tcatcattat aaatatcatt aatagaagat tgacccccgg tataatatgt
      541 aagatctatc gctgcccaca ttattggatt gaaattataa ctaatatgtg tttgtagagc
      601 aatcaagggg tcctgaatga tgagaaactg tatgaattat attcaaacag attaattttt
      661 tgatccgaat tttgtcggag atagtaaaaa agctgaagag aaagaattaa tgattccaat
      721 aaataacaaa ggggcacaac ggccccttca ttattaaaaa ttaaaataat taattactgc
      781 ccgtaacttc ttatataaag agaatcataa tgtcctttat gatcgaatac ttcattggtt
      841 tttgagtagt gcatccattc ttcaatcagt tcttctttta tttcggggtg ctgatctgag
      901 acatcattaa tttctccggg gtcaacttct aaattgtata attgccagta gcctgcacca
      961 aacggctgtg gcaatctcag tattttccat tttcctttta tgtaagcttt catttcaaac
     1021 agctcgtaac ccatgcctgt gttatcatga acagattgta ctttcccagt aaggataggc
     1081 aaaatagatt taccaataag accgtgaatc ttgtgtccgt taaactcttc aggataagtt
     1141 gccccagtca attccagcaa cgttggcatt atatcagtaa cgtgaacaaa gctgtaattc
     1201 cattttcccg gagtattatt tgtaccgggt gtttttatta ttaatggagc tctaattcct
     1261 ccttctgttg tgaaggtctt aaataatctg agaggcgatg aaacagcttg tgcccagcct
     1321 ggacccattg caatatatga tccctttagg ccgcgattat ccatttcatt gttaaatgtg
     1381 ctaagatact ttccatcttc attgccggga taagttgaag ctactgcccc gttagcaccg
     1441 ttatcagaaa agaaaaaaat catagtgtta tcgtacattc cattttcttt aaggtaattg
     1501 attacccgac cgatactcat atcaacatac tcaaccatag cagcatacac ttccatatcg
     1561 cgtgcaaatg cctgctgctg ttttttactc aatgtttccc attttggaat catcgggttc
     1621 ggagcgaaat tatttagttg ctcgggcatc aaacccaagg cttttaaatt ttccaggcga
     1681 gaaagtctaa gtgcgtccca cccgtcatca aatttacctt tatatttttt tatatactct
     1741 tcaggggcgt gaagcggatc gtgaggtgcg gtgtatgcca ggtaagcgaa aaatggcttt
     1801 ttatcttctt tgtgcatgtc aataaaatga agcaacgaat ctgtgtaatt tttagttgaa
     1861 taaaaatcag taggaagttc aacttctttt ccgtttcttg tataatccat atgttgtaag
     1921 ggagacaacg ctctctcatc attaaaatga ctgccgccgc cagttcccag aataaatgtt
     1981 tcttcgaatc ctcgatcaaa aggattttca ccttgctctt ctccaagatg ccatttaccc
     2041 gtcatataag tgttgtaacc aacagtgcgt aatagttcag gaatggtaac aacctgttta
     2101 cttaaggcac cagcatagcc gggaagattt aattctcgca gctgcggata atctagctcg
     2161 ctcataatgc caagtcccgc aacgtgatta tcatttcctg ttaataatgc cgaacgagtt
     2221 ggagagcagg taggtaatac atgaaagtta gcaaataaca ttcctcctct agcaagctta
     2281 tctatttccg gagtcttgat aattttactt ccaaaaggtt caatatcgct aaagcccaaa
     2341 tcgtcagcaa ctatcagaag aatattcggt ttattagaat tggattgcat cccttctttt
     2401 tctggagatg gtttacaact gaaaagtatt aataacgaaa agatgaacaa gagatttcta
     2461 gaaattgaaa tgtagttcat atacatcttc ctttttattt atttaaataa atatagaaaa
     2521 aaaatgtaaa cctggaaaag catttcgcta gtaattgtta ttcttttgca ttcttaattg
     2581 atagacctca atattttatt tgtttctcct aaactccacg taaaaagtta aaccctcaac
     2641 ggcctgaaaa taatcaccga caagcaaatc tctttcaggt atgtaagtta aattataatt
     2701 tgagccggga taattttcat cccgtaattc aatatatact tttaaaaatc cttcagtata
     2761 ggcccactct gctttgctga cattgattgg tcttggatta aaatattgtg cactcataac
     2821 tccattttca gaaacatctg atattttaat ttgatactca gcatctgttc ttatccagct
     2881 cccggcgata atattcttat caattgatgt ggatatggtt tcagattctt ctacagtttc
     2941 ggtgatggag ctgtgaaaaa agataaagta tgatgcaatg agtgcggcaa taattaaaat
     3001 tgctgcatag aatattgatt tgttcttttt catttatttt taattcggat gattttataa
     3061 agatttaatt atttgcaatt aaataataaa taattttcag atgatttact tatttacatt
     3121 tattcaaagc aaatgttttt aagatctttt ataaatatct tccatttacg ttgtcaaaat
     3181 tggagtagca aggatgctga aatcttattt ttaagctcct taaatgaata gatttactat
     3241 atctacatat aggaaataaa aaaataagta ttagatttaa accacaaact aaataatttt
     3301 ttttagtgct tagtaaaaaa ataactggat aaatatatga gattcatttt acgatctctt
     3361 acaacgctgc
//
LOCUS       DASWBY010000363         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_467464, whole genome shotgun sequence.
ACCESSION   DASWBY010000363 DASWBY010000000
VERSION     DASWBY010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467464"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1213)
                     /locus_tag="VF870_06680"
     CDS             complement(71..1213)
                     /locus_tag="VF870_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9251909.1"
                     /translation="MALFFIIFFTVYTSLNFYVFLRGWQVISAYPVLKPVYAVLFVIF
                     AYGYVLAKVLYKFLHPLAYDILLGVGAIWFAFLAYFILSLLLIDIVRLFNGWFHFFPS
                     IITNNYETTKRFTAIIVIALVGLIVFLGNLNKRNIEIRNLEITIPKGDSKLDELNIVM
                     ASDIHLSPIDGERLLVKIVDKMNSLNPDIILLAGDIVDDKAKVLDERGIGESFRKLKS
                     KYGVYSINGNHEFINGVDSCVRYAEKFGIKFLRDGYELIDSSFYVIGREDSSMPQFTG
                     KQRKSLEEIVKVIPSNYPKILLDHTPFKLEQAQQNKIDLQLSGHTHHGQIWPANLITK
                     MIYEISWGYKKKGNTHYYVSSGAGTWGPPVRTGSSSEIVNIKVKFE"
     gene            1391..1543
                     /locus_tag="VF870_06685"
     CDS             1391..1543
                     /locus_tag="VF870_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251910.1"
                     /translation="MTREKVFKSKNPLPVDSHSASFTRSGFSSERHPAMDKYKKSSFI
                     FAQDDD"
     gene            complement(1547..>2468)
                     /locus_tag="VF870_06690"
     CDS             complement(1547..>2468)
                     /locus_tag="VF870_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX9251911.1"
                     /translation="SEKISNIREMEDAGAGAVVLYSIFEEQIEHEQLELDYHTSAHTE
                     SFAEATSYLPEPFEYKLGPDEYLNHIRKAKEAVDIPIIASLNGKSIGGWTDYAKKMEQ
                     AGADALELNIYLLPTDVKKSGSEVEKTYIDIVKAVKSNVKIPFAVKMHPFFSSTSYMA
                     TELSNAGASGLVMFNRFYQPDIDLETLDVVPNVILSTPMAMRLPLRWIAMMYGKVNAD
                     LAATSGIYSAEDVLKMVMAGAKVTQMLSALLKFGIGHIADVTTNLKSWMEEKEYESIE
                     QMRGSMSYMNVDDPSKFERANYMKVLNSYK"
BASE COUNT          781 a          469 c          417 g          801 t
ORIGIN      
        1 taacggtgat gttttagttc aaaatagcaa acccgattca attgcagaat cgggtttgtc
       61 tgtatacttg ttactcaaat ttcactttga tatttacaat ctcagaactg cttccagttc
      121 ttactggcgg tccccacgta cctgcaccgg aagaaacgta gtaatgtgtg ttgccttttt
      181 tcttgtaacc ccagctaatt tcataaatca tttttgtaat taagttagcc ggccatattt
      241 gtccgtgatg tgtgtgtccg gaaagttgca aatcgatttt attttgctga gcctgctcta
      301 atttaaaggg agtatggtct aataaaattt tcggataatt agatgggata acttttacaa
      361 tctcctctaa agattttctt tgcttacctg taaactgtgg cattgagctg tcttctcttc
      421 caattacata aaaactgctg tcgatcaatt catatccatc gcgtaagaat ttaattccaa
      481 acttttcagc atatcttaca caggaatcaa ctccgtttat aaattcgtgg ttaccattaa
      541 ttgaatagac accatattta gattttaatt ttctgaatga ttccccaatt ccccgctcat
      601 ccagaacttt tgctttatca tcaacaatat caccggcaag taaaattata tccggattaa
      661 gtgaattcat tttatctaca attttaacaa gcaatctttc gccgtcaata ggggagaggt
      721 ggatatcaga agccataaca atgtttagtt catcaagttt gctgtcacct tttggaattg
      781 ttatttcaag gtttcttatt tctatatttc gtttgttcag atttcctaaa aaaacaatca
      841 agccaactaa agcgattact ataattgccg taaatctttt tgtggtttca taattgtttg
      901 taatgattga agggaaaaaa tgaaaccagc cattaaacag tcttacaatg tcaatcagta
      961 ggagtgacaa aataaagtaa gcaagaaaag caaaccagat tgcaccaact cctaataaaa
     1021 tatcataagc aagaggatga agaaatttat aaagcacttt tgcaagtaca tatccgtatg
     1081 caaatattac aaataaaact gcataaacag gctttaaaac cggataagca gaaataacct
     1141 gccagccgcg taaaaacaca taaaaattaa gcgatgtgta aacggtaaag aaaatgatga
     1201 aaaataaagc cattgtcctc taatatttta ttatttcaaa caaaagatag ggagataagt
     1261 tcaatttgat tttggtgaga atcacagcta tgagaactca atcttgatgg tcatttcgac
     1321 gactgaaaag aggagaaatc tatgcaaata aaaccaagaa gatttctcac cgactaacgt
     1381 cgggttcgaa atgacaagag aaaaggtttt taaatcaaaa aatccactgc cggttgattc
     1441 acattctgct tcttttacaa gatcaggatt ctcctctgag cgtcatccgg caatggataa
     1501 atataaaaag tcatccttca tcttcgctca ggatgacgat taagttttat ttataagagt
     1561 taagcacctt catatagttt gcacgttcaa acttggatgg atcatcaaca ttcatgtagc
     1621 tcatgcttcc gcgcatttgt tcaattgatt cgtattcttt ttcttccatc caggatttta
     1681 agtttgtggt aacatcggca atgtgaccaa taccaaactt taacagagca gataacattt
     1741 gggtaacttt tgctccggcc ataaccatct ttaaaacatc ttcagcggaa taaattccgc
     1801 ttgtagcagc aagatcagca tttacttttc cgtacatcat tgcaatccat cttaatggta
     1861 atctcattgc catcggagta gataaaatta cgttcggtac tacatcaaga gtttctaaat
     1921 caatatcagg ctgatagaaa cggttgaaca taaccaatcc gcttgcacct gcgttgctaa
     1981 gttcggttgc catatagctt gtggagctaa aaaaaggatg catcttaacc gcaaaaggaa
     2041 ttttaacatt tgattttaca gccttaacta tatcgatata ggttttttca acttcgcttc
     2101 cggatttttt tacatctgtt ggaagcaaat aaatatttaa ctcaagtgca tctgcaccgg
     2161 cttgttccat cttctttgcg tagtctgtcc agccgccgat tgatttacca tttaagcttg
     2221 cgatgattgg aatatcaact gcctcttttg ctttgcggat gtgatttaaa tattcatcag
     2281 gaccaagttt gtactcgaat ggttctggta agtaagatgt tgcttctgca aaactttcag
     2341 tatgagcaga agtatgataa tctaattcta actgttcatg ctcaatttgt tcttcaaaga
     2401 ttgagtaaag aaccactgct ccagcaccgg catcttccat ctcacgaatg ttagaaattt
     2461 tttctgac
//
LOCUS       DASWBY010000364         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_16883, whole genome shotgun sequence.
ACCESSION   DASWBY010000364 DASWBY010000000
VERSION     DASWBY010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16883"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /gene="hrcA"
                     /locus_tag="VF870_06695"
     CDS             complement(<1..647)
                     /gene="hrcA"
                     /locus_tag="VF870_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX9251912.1"
                     /translation="MLYEDLNDREKTILRSIVQQFILTASPVGSRNITKRFDVGVSPA
                     TVRNIMSDLEESGFINHPHTSAGRIPTDKGYRYYVDALMEIQKLKSSDKGYIDKSLDK
                     NILETDEILKIASKLLSTITKQIACVTYPTLDNGILEKIQLVILSSTRLLVVVSIKSG
                     LVKTMTLEFDTEVENEKLQKVEALLNERLNGLTFQEIRKTFKERLSDVGDNEKPI"
     gene            840..1835
                     /gene="purM"
                     /locus_tag="VF870_06700"
     CDS             840..1835
                     /gene="purM"
                     /locus_tag="VF870_06700"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX9251913.1"
                     /translation="MDQTYKSAGVDIEAGERTVDKIKGYAKSTFNKSVLADIGMFGAF
                     YEPDFSGYKNPVLVSSVDGVGTKLKIAIEMDKHDTIGQDLVNHCVNDIAVCGAKPLFF
                     LDYYATGKLNPDKAADVIKGFSIACKENECALIGGETAEMPGLYDENDYDVSGTIVGV
                     VDKSKIIDGKRIQKGDLLIGFPSNGLHTNGYSLARKVLLEKFNVNDQLDEINNVLGDE
                     LLKIHKSYLKLITALKNSVDVKGLSHITGGGIIGNTSRIIPKGLNLLIHWGAWELPAI
                     FKLIQNTGDISSEEMHKVFNCGIGLIAVISKEDKLQAFQIAHDAHEHPLLIGEVI"
     gene            1836..>2804
                     /locus_tag="VF870_06705"
     CDS             1836..>2804
                     /locus_tag="VF870_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9251914.1"
                     /translation="MFIDTHAHLFYENFKDDVDDVIKRSKENAVDYIVVPATDIKTAK
                     EALRLAEKYEQIYATVGTHPHDTKDWNESLLKEIEELAKHPKVIAIGEIGLDYYYDFS
                     PKEQQIKAFKSQLDLAIKLELPVVIHNRDSDEDMMDIILSYCGTGLKAQFHCFNGSIN
                     DAFEYMKMNHFISFTGNITYKKSDSLRETLKHINQNHLMLETDSPFMTPVPFRGKRNE
                     PAYVSYVAQQVADVHKISLEDVGRITSLNTFRFFGIGKAPKTNFTYLIGNSLYINVTN
                     RCNADCTFCRRKEDPFLNGYNLGMRKSEEPLAEVYISEIGDPKKYEE"
BASE COUNT          973 a          415 c          492 g          924 t
ORIGIN      
        1 attattggtt tttcattatc accaacgtca gataatcttt ctttaaaagt ttttctaatt
       61 tcctggaaag ttaatccgtt taatctttca ttcaacaatg cttcaacttt ttgaagtttt
      121 tcattttcaa cttctgtatc aaattctaga gtcattgttt taactaatcc ggatttaata
      181 ctaactacaa caagcaatct tgttgatgat aatataacta attgaatttt ttccaaaatg
      241 ccattatcca aagttggata agtaacacag gcaatttgtt tggttatcgt acttaacagc
      301 ttagatgcaa ttttcaatat ctcatcagtt tccagaatgt ttttgtccag acttttatca
      361 atataacctt tatcagaact ttttaatttc tgaatctcca tcagtgcatc tacataataa
      421 cgataacctt tgtcggttgg aattctacct gccgaagtgt gtggatgatt aataaaacct
      481 gattcttcta aatcggacat aatattcctt acagttgcag gggaaacacc aacgtcaaac
      541 cgtttagtaa tattccttga accaaccggc gaagcagtta agataaactg ctgcacgatt
      601 gatctcaaaa tcgttttttc tctatcattt aagtcttcgt aaagcataat tcaaacattt
      661 taacaaaaaa tttaattttc aaccatcaaa tatatcaata aatgaaaaaa tatccgcaga
      721 atcaatatgt tataaaatat caaatattta gactttttta ggttttgtat aaaatagtta
      781 ttagtggtaa atttttttgt gaaattttaa tcatctttac aagaaataga gagtgaattt
      841 tggaccagac ttataaatca gccggagttg atattgaagc aggcgagaga actgttgata
      901 aaataaaggg atatgcaaaa tctacattta ataaaagcgt tttagctgat attggaatgt
      961 ttggggcgtt ttatgaacct gatttttcag gatataaaaa tccggttttg gtcagcagtg
     1021 tggatggtgt tggaaccaaa cttaaaattg ccatcgaaat ggataagcat gatacaattg
     1081 gacaggattt ggttaatcat tgtgtaaacg atattgctgt ttgtggtgct aaaccgcttt
     1141 tctttttgga ttattacgcc acaggaaaat tgaatccgga taaagctgct gatgttataa
     1201 aaggtttttc aatagcgtgt aaagaaaacg aatgcgcatt aatcggcggc gaaactgctg
     1261 aaatgcccgg tctgtatgat gaaaatgatt atgatgtatc tggaaccata gtaggagttg
     1321 ttgataaatc aaaaattatt gacggcaaaa gaatacaaaa aggagattta ctgattggtt
     1381 ttccttcaaa tggtttgcat acgaatggat attctctcgc aagaaaagtt ttattagaaa
     1441 aatttaatgt taacgatcag cttgatgaaa taaataatgt gcttggtgat gagctactaa
     1501 aaattcacaa atcatatcta aaactaataa ccgctttaaa aaattctgta gatgttaaag
     1561 gtttatctca cataacaggc ggcggaatta ttggaaatac ttcgcgcatt attccaaaag
     1621 gactgaatct actaattcat tggggcgcct gggaattgcc agcaatattt aagttaattc
     1681 aaaacactgg cgatatttcc agtgaagaaa tgcataaagt atttaattgc ggaattggat
     1741 taatcgcagt gatatctaaa gaagataaat tacaggcatt ccagattgca cacgatgcac
     1801 acgagcatcc attattgatt ggtgaagtga tttaaatgtt tattgataca catgctcatt
     1861 tattctacga gaattttaaa gatgatgttg acgacgtaat aaagagatcg aaagaaaatg
     1921 cggttgatta tattgtggta ccagcaactg atattaaaac cgctaaagaa gcactccggc
     1981 ttgctgaaaa atatgaacaa atttatgcca ctgtaggtac tcatccgcac gatacaaaag
     2041 attggaacga atctttacta aaagaaattg aagaacttgc caaacatcca aaagtaattg
     2101 caattggtga aattggttta gattactact atgatttctc accgaaagaa caacaaatta
     2161 aagcatttaa atcccaactt gatctggcga ttaaacttga acttccggtt gtaatacata
     2221 atcgtgactc agatgaagat atgatggata ttatattaag ttattgcggt acaggattaa
     2281 aggctcaatt tcattgtttt aacggatcta tcaatgatgc ttttgaatat atgaagatga
     2341 atcattttat ttctttcaca gggaatatca cttataaaaa aagtgattct ttgcgagaaa
     2401 ctttaaagca tatcaatcaa aatcatttga tgcttgagac tgattcacca tttatgacac
     2461 cggtaccttt tcgaggtaaa agaaatgagc ctgcctatgt aagttatgtt gctcagcaag
     2521 ttgctgatgt tcataaaata tctttggaag atgttggtag aataacttca ttaaatactt
     2581 ttagattttt tggaattggt aaagcaccaa aaactaattt cacttatttg attgggaatt
     2641 ctttatacat aaacgttaca aaccggtgta atgcggattg tactttttgc agaagaaaag
     2701 aagatccatt tttaaatggt tataatcttg gaatgagaaa atctgaagaa ccgcttgctg
     2761 aagtttatat tagtgaaatc ggagatccta aaaaatatga ggaa
//
LOCUS       DASWBY010000365         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_539335, whole genome shotgun sequence.
ACCESSION   DASWBY010000365 DASWBY010000000
VERSION     DASWBY010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539335"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF870_06710"
     CDS             complement(<1..372)
                     /locus_tag="VF870_06710"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9251915.1"
                     /translation="MQKLLLISAIILLSNSLFAQDLTGYIYGKVYDASTKQAIPFANV
                     IVIGTNNGAATDENGYFKITDLPVNTYQVRASVVGFNPQTKTDVVVQTSKPAEIYFEL
                     VPQAIELQGVTVTSDYFRKDPL"
     gene            512..2077
                     /locus_tag="VF870_06715"
     CDS             512..2077
                     /locus_tag="VF870_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9251916.1"
                     /translation="MTKKKNNLTSKVSDKKNKDAFLSGITAKKLITEPQNSVLCEIAV
                     SFDDQFHYQLKTEGDKLEIFYSPSVKKVTGYTPAELIKLPSLGREMVYEEDIPDVKKK
                     LHKIENGKISSLELLYRYNRKDGKIIWLKEQVKLEVIDNQKIIRGLVLDVTNFKKSES
                     DYGEIINNLSSEITARDNFLSILSHDLRAPFSSILGFTEILLSETSLSDAERTEYLNY
                     INDSSTNQLQLVNYLLDWSNLQTGRMHMEPHRVQAQGLVFNCISALTGVAMRKNIDIK
                     VNVPDSIFIEADERLMLQVITNLVSNSIKFSEEGKTIRISVERFNNELVEFVISDEGV
                     GIPDNYHQRIFKFEKMFSTRGTRGEKGTGLGLSLVKEIIEKHKGQIWFYSKEKVGSEF
                     HFTVPSSENTILLVEHNQNDFIQLEKIIRDNFPEFKFVGTDNGFEAINVVLKQHPSLI
                     ISSHDLPLMNGIQFVKSILRGDKKYYAPIIALIDSADESLLKTYHEIGIKSVLSKPVN
                     IKQFNREVSLALN"
     gene            2158..3489
                     /locus_tag="VF870_06720"
     CDS             2158..3489
                     /locus_tag="VF870_06720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX9251917.1"
                     /translation="MNLAVIGTGYVGLVSGTCLAETGNNVICVDVDEKKIETMKNGQI
                     PIYEPGLEVLFLRNIVKKRLSFTTDLHAAVKNSSVIFLCLPTPQGGDGAADLKYVLKV
                     ADDLGQMFRDEPDLGFKVIVDKSTVPVGTSDRVRAAIKKHAPNFNFDVCSNPEFLREG
                     IAVEDFMKPERVIVGTSNEKTKKVMEQLYEPFVRTGNPVYFMDEKSAEMTKYAANSFI
                     AMKISFMNEIARLCELTGAQVDNVRLGIGSDSRIGKRYLFPGIGYGGSCFPKDVHALV
                     NTANENKYDFKILKSVIDVNYDQVKHFFIKISKHYNGNLKGKHFALWGLAFKPNTDDV
                     REAPAHYLIKLLLEAGAAIKAYDPEANNTTKAVLGDVIQYAETSGETLKDADALIVVT
                     EWKEFRNPDFDVIRKSLKAPVIFDGRNLYDLDKLNELKFTYYSIGRSDVKN"
     gene            3528..4529
                     /locus_tag="VF870_06725"
     CDS             3528..4529
                     /locus_tag="VF870_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9251918.1"
                     /translation="MLNQSELNSLSKGDSIDHFFLVKKCEIRLTKAGKEYLSLELGDK
                     SISSAANLWDDVSGFKSIKNSLALGDIVKITGSMDEYQGNSQIKIDSIRLAKQSDDVT
                     PKDFLPKSLRDLNVMKKEFTARMEKIADNDLKNLMNNILSEKRFVKYTSVPAGKMWHH
                     GYISGLIEHTLEIIKICDLMCDIHPQINRDLLISGAMLHDFGKIEELSFEPVFEYTDK
                     GKLLGHIVIAAMIVNDEINKIPDFPENLKNNLLHLILSHQGKLEYASPVVPKTLEAIT
                     LYQADELSAKVNAYKNVIANEIKSGSNWTKFISLAGTDIHSHGLVNKSDIDKKTLFD"
     gene            4552..>5013
                     /locus_tag="VF870_06730"
     CDS             4552..>5013
                     /locus_tag="VF870_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251919.1"
                     /translation="MKKILQIASILLLVTFGFTASFAQSVDELLKEGDQYVAEFNHQK
                     ALDTYLKADKLSPNNWNVLWRISRAYVDLGEKMPEKTDEQKAEQEKVYKKAFEYADKA
                     VKNGPDQSITYVRRAIANGRIALFQGIFSAPGTVNDVKKDCEKAIQLGNGDN"
BASE COUNT         1786 a          747 c          939 g         1541 t
ORIGIN      
        1 aagcggatct ttacggaagt aatcacttgt tacggtaact ccttgcagtt cgattgcttg
       61 cggtacaagt tcaaaataaa tttcggcagg tttgctcgtc tgaactacaa catcagtttt
      121 tgtttgcgga ttaaatccaa caacagatgc gcgaacctga taagtgttaa cagggagatc
      181 ggtgatctta aaatatccat tttcatctgt tgccgcacca ttgtttgttc cgataactat
      241 cacattggca aacggtattg cttgctttgt tgaagcatca taaacttttc catagatata
      301 accagttaaa tcctgcgcaa ataaagagtt ggataataga ataattgcag atattaataa
      361 taatttttgc atgttttttt tgctttattt taatttatga gtaagtaaaa acaattttgg
      421 agtgataaag ttcctttttg cttaacttat aacgttttat cccaaaaagt aaattgttat
      481 atttttaatc aagtgtttta aaaaataaaa gatgaccaag aaaaaaaata atttaacatc
      541 aaaagttagt gataagaaaa ataaagatgc atttctttcc ggtatcacag ccaagaaatt
      601 gattacagaa ccacaaaatt ctgttttgtg cgaaattgcc gtctcatttg atgatcagtt
      661 tcactaccag ttaaaaaccg agggtgataa attagaaata ttttattctc caagcgttaa
      721 aaaagttacg ggttatactc cagcagaact tattaaactt ccatcacttg gcagagagat
      781 ggtttatgaa gaagatatac cagatgtaaa aaagaagttg cataaaatag agaatggcaa
      841 aatctcaagt ctggaattac tgtaccgtta caaccgtaaa gatggaaaga ttatctggtt
      901 aaaagaacaa gtaaaattag aagtcataga taatcagaaa attattagag ggttggtttt
      961 agatgtaaca aacttcaaaa aaagtgaaag tgattacgga gagattataa ataatctctc
     1021 aagtgaaatt acagcacgcg ataatttcct gtcaatattg tctcacgatc ttagagcccc
     1081 tttttccagc atacttggct ttacggaaat tctattaagt gaaacatctt tatcagatgc
     1141 tgaacgtaca gagtacctga attatataaa tgattcatca accaatcagt tacagcttgt
     1201 aaattatcta cttgattggt caaatcttca aacaggaaga atgcatatgg aaccgcaccg
     1261 tgtgcaggcg cagggattag tttttaattg catctctgca ttaaccggtg ttgcaatgag
     1321 aaaaaatatc gatataaaag ttaatgttcc tgattctatt tttattgaag cagatgaaag
     1381 actaatgctg caggttatta ccaatcttgt gagtaattca attaaatttt ctgaagaggg
     1441 gaaaacaatt agaatttctg tggagagatt taataacgag cttgtggaat ttgtaataag
     1501 tgatgaaggg gttggaatac cggataatta ccatcaaaga atttttaagt ttgaaaagat
     1561 gtttagtaca agaggaaccc gtggagaaaa aggcacaggg ttaggattat ctttggtaaa
     1621 ggaaattata gaaaaacata aagggcaaat ctggttctac tcaaaagaaa aagttggaag
     1681 cgaatttcac tttacagtac caagttccga aaacacaata ctgttagttg aacataatca
     1741 aaatgatttt atacaattgg agaaaattat tcgggataat tttcctgaat ttaaatttgt
     1801 tggaactgac aatggatttg aagcaataaa tgttgtacta aaacaacatc catcattaat
     1861 aatttcatca cacgatttgc cgcttatgaa cggaatacag tttgttaaat ccattttgcg
     1921 cggtgataaa aaatattatg ccccgataat tgccctgata gactcagcag atgaatcgtt
     1981 attaaaaact tatcacgaaa taggaatcaa atcagtactc tctaaacctg ttaatataaa
     2041 acagtttaac agagaagttt cgttggctct taattaatca aataaatttc tgtaattcga
     2101 attgaaaaag actttcgtta tttttcttaa gccaaataat ttttatcgga ggaacttgtg
     2161 aatcttgcag taatcggaac tggttatgtc ggtcttgttt caggtacttg tcttgcagaa
     2221 accggaaata atgtaatttg cgttgatgtt gatgagaaaa aaattgaaac aatgaaaaac
     2281 gggcagatac caatttatga acccggactt gaagtattat tcctaagaaa catagttaag
     2341 aaaagattat catttacaac agatttacac gcagcagtaa agaattcaag cgtaatattt
     2401 ttatgccttc caactccgca agggggtgat ggtgctgccg atcttaaata tgtcttaaaa
     2461 gtagctgatg atctgggaca aatgttcaga gatgaacctg accttggttt taaagtaatt
     2521 gttgataaaa gcacggtccc ggttggaacg agcgacaggg ttcgtgcagc aatcaaaaag
     2581 catgcaccaa attttaattt tgatgtttgt tctaatcccg agtttttgcg tgaaggaatt
     2641 gctgttgaag attttatgaa gcctgaaaga gtaattgttg gaaccagcaa tgagaaaact
     2701 aaaaaggtaa tggaacaact ttacgagcct tttgtgcgca ccggaaatcc ggtttacttt
     2761 atggatgaaa aatctgctga gatgaccaaa tatgctgcaa actccttcat agcgatgaaa
     2821 atttctttta tgaatgagat tgcaagattg tgtgaattga caggagccca agttgataac
     2881 gttagattag gtatcggatc ggattccaga attggtaaaa gatatttatt tcccggaatt
     2941 gggtatggtg gttcttgctt tcctaaagat gttcatgcac ttgttaatac tgcaaatgaa
     3001 aacaaatacg actttaaaat tttaaaatcc gtaattgatg ttaattacga tcaggtaaaa
     3061 catttcttta ttaaaatatc caaacattat aacggaaatc ttaaaggcaa acactttgca
     3121 ctttggggat tagcattcaa gccaaacacc gatgatgttc gtgaagctcc tgctcactat
     3181 ctcataaaat tattattgga ggcaggtgct gcaattaaag cttacgatcc cgaagcaaat
     3241 aacacaacaa aagctgtact tggtgatgtt attcaatatg cagaaacttc cggagaaact
     3301 ttaaaagatg ctgatgcact tattgtagtt acagagtgga aagaattccg taatccagat
     3361 tttgatgtaa taagaaaatc gttaaaagct ccggttattt ttgatggaag aaatctttat
     3421 gatctcgata aacttaacga attaaagttt acttactatt caattggtag aagcgacgtt
     3481 aagaactaaa aattattttt tatttcagtt atgatttcaa aatagaaatg cttaatcagt
     3541 cagaactaaa ctcgttatca aagggtgatt ctatagatca tttttttcta gtaaaaaaat
     3601 gtgaaataag attaaccaag gcaggcaaag aatatctttc attagaactt ggtgataaat
     3661 caatttcttc tgccgcaaat ctctgggatg atgtttcagg attcaaatca ataaaaaatt
     3721 cattagcact tggcgatatt gttaagatca ccggctcgat ggatgaatat cagggtaatt
     3781 cacaaattaa aattgattca atccgattag caaaacaaag tgatgatgtg accccaaaag
     3841 atttccttcc caaatccctg cgtgatctta atgtgatgaa aaaagaattt acagctcgaa
     3901 tggaaaagat tgcagataat gatttaaaaa atcttatgaa taatattctt tctgaaaagc
     3961 gatttgtaaa gtatacttct gttcctgccg gtaaaatgtg gcatcatggt tatataagcg
     4021 gattgattga acacacactg gagatcataa aaatttgtga tttgatgtgc gatattcatc
     4081 cacagattaa tcgtgatctt ttaataagcg gagcaatgct tcatgatttt ggcaagattg
     4141 aagagttaag ttttgaaccg gtctttgagt acaccgataa aggtaaactg ctcggtcaca
     4201 tagtaattgc cgcaatgatt gttaatgatg agataaataa aattcctgat tttcctgaaa
     4261 acttaaaaaa caatctgctt cacttaatat taagccatca aggaaagctt gagtatgcat
     4321 caccggtggt gccaaaaact ttagaagcaa ttactctgta tcaggcagat gaattaagtg
     4381 caaaagtgaa tgcgtataaa aatgttatag caaatgaaat taaatccgga tcaaactgga
     4441 caaaatttat ttctcttgct ggtacagaca ttcattctca tggtttagtt aataaatcgg
     4501 atattgataa aaagaccttg ttcgactaat caaataaatg aaggaacaca catgaaaaag
     4561 attctacaaa tcgcttcgat cctgctgctt gttacttttg gttttacggc ttcttttgct
     4621 caatcggttg atgaattatt aaaagagggg gatcagtacg ttgcagaatt taatcatcag
     4681 aaagctttag atacatatct taaagcagat aaactttctc caaataactg gaatgtttta
     4741 tggcgtataa gcagagcata tgttgatctt ggtgaaaaga tgcctgaaaa aactgatgaa
     4801 caaaaagcag aacaggaaaa agtttataaa aaggcatttg agtatgctga taaagctgta
     4861 aaaaatggac ccgatcaatc tataacttat gttagaagag caattgcaaa tggaagaata
     4921 gctttatttc aaggaatctt ctccgcgccg gggacagtaa atgatgtaaa aaaagattgt
     4981 gaaaaagcca ttcaattagg aaatggagat aat
//
LOCUS       DASWBY010000366         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_692572, whole genome shotgun sequence.
ACCESSION   DASWBY010000366 DASWBY010000000
VERSION     DASWBY010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692572"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VF870_06735"
     CDS             complement(<1..205)
                     /locus_tag="VF870_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251920.1"
                     /translation="MIIPSIYGSEIRVKNIFYPEFFSFEIKYIYFRFVIKQHSLIKKR
                     GLFMAAKPMTKGQILDHMAKKTGV"
     gene            300..>1960
                     /locus_tag="VF870_06740"
     CDS             300..>1960
                     /locus_tag="VF870_06740"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9251921.1"
                     /translation="MFRNTLVLVIIYSVIALGQIQDSVTSEIRFYPDTLFSQPDSVIS
                     NDSLTIETKRDSIVSLYSSVLTETSFIIPNKELLKLEYQYTGDYLRLSPFNFIKNLGF
                     TGQPNETFLYGVGNNAISYLMDGISYNDRQSNSLNLNLIQSEDVDSIEMIPLPRGFLY
                     GAYNNPVSVNFITKDFIPILPYSRIRFYQGTNRDMMFDGIFNAVVTNKLIASFDITNR
                     IYDGTYDNTDYSIWQGKIKLRYLLSNAVNIIASYSYNDYNAGYSGGVDVDSIKSLGEN
                     VDNVLYDFRAAPMVYPNGELKTLTHLPRLRFLIKPTEWMKSDASLFFMFNRYEKNTST
                     SDYSESKTYGLNIRNDFDYNIFNFQLNIDYEKTKNLLEGSYYTIIDTVQPPSYQDEIK
                     FNMFSIAGIVSADLLDSTIIPSIYLKTSTYNNELYNSSLESNFKEKNSSGYGADLLLK
                     LKPNLSFYFGVSHINPYNIDDLDYNVIEIGGYFSSEFINADLKYFINNYSDDFFVYES
                     GNTSNYVPGDVNGLGLNLKFNYWKLLLESNSSYYFAIDNDKLIGVP"
BASE COUNT          655 a          282 c          320 g          703 t
ORIGIN      
        1 taacgccggt cttctttgcc atatgatcaa gaatctgacc tttggtcatt ggttttgctg
       61 ccataaatag ccctctcttt tttattaatg aatgttgttt aattacaaag cgaaaataaa
      121 tatattttat ttcaaatgaa aagaattccg gataaaaaat atttttaacc cttatttcac
      181 taccataaat tgagggtata atcaatccct aattcagccg atgctacaca aatagctttg
      241 atgcacaaat gttaatttgt tattttaatc tttaataaat aaaattgaat taatttttaa
      301 tgttcaggaa tacattagta ctggtaataa tttattctgt tatagctctt gggcaaattc
      361 aagattcagt tacttctgaa ataagatttt atcccgatac gcttttcagt caacccgact
      421 cagttatctc gaacgattca ttgacgattg aaacaaagcg agattctatc gtttctctgt
      481 attcttctgt gcttacagag accagcttta ttattccaaa taaagaattg cttaagcttg
      541 aatatcagta caccggggat tatttaagac tatcgccgtt taactttata aagaatttag
      601 gatttacagg acaaccaaat gaaacatttt tatacggagt gggaaataat gcaataagtt
      661 atttgatgga tgggatctca tataatgatc ggcagtctaa ttcgttgaat cttaatctta
      721 ttcaaagtga agatgttgat tcgatagaga tgattccttt gccgcgcgga tttctctatg
      781 gcgcatataa caatcctgtt agtgtaaatt ttatcacaaa agattttatt ccaattctgc
      841 cttattcacg aatcaggttt tatcagggca caaacagaga tatgatgttt gatggaattt
      901 ttaatgcagt tgtaaccaat aaacttattg cttcttttga tatcacaaac agaatttatg
      961 acgggactta tgataatacg gattattcga tctggcaggg aaaaattaaa ttaagatatt
     1021 tgctttctaa tgccgtaaac attatcgcat catacagcta taatgattat aatgccggat
     1081 acagcggggg agttgatgtt gacagtatta aatcacttgg agaaaatgtt gataatgttt
     1141 tgtatgattt cagagctgct cctatggttt atccaaatgg cgaattaaaa actttaactc
     1201 atttaccaag attaaggttt ttaatcaaac caacggaatg gatgaaatca gatgcaagtt
     1261 tattttttat gttcaatcga tatgaaaaaa atacatcaac aagcgattat tcggaaagca
     1321 aaacttatgg tttaaatatt cgtaatgatt ttgattacaa cattttcaat tttcaactaa
     1381 atattgatta tgagaagaca aaaaatcttc ttgaaggttc ttattataca attattgata
     1441 cggttcaacc tcctagttat caagatgaaa ttaaatttaa catgttttcg atagctggca
     1501 ttgtatctgc agatctactc gatagcacaa ttattccctc aatatatttg aagacatcta
     1561 cttacaataa tgaattatat aattctagtt tggaaagtaa ttttaaagaa aaaaatagca
     1621 gtggttatgg cgctgatcta ttgctaaaat tgaagcctaa tcttagtttt tatttcggtg
     1681 tttctcatat aaatccttat aacatagatg atttagatta caatgtgatc gaaattggtg
     1741 ggtatttttc gagtgaattt attaatgctg atctgaaata ctttattaac aattatagtg
     1801 atgatttttt tgtatatgag tcgggtaata caagcaatta tgttcctggc gatgtgaatg
     1861 gattgggatt aaatctaaaa tttaattact ggaaattgct gctggaatca aactcatcat
     1921 attactttgc tatcgataat gataaattaa ttggtgttcc
//
LOCUS       DASWBY010000367         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_578345, whole genome shotgun sequence.
ACCESSION   DASWBY010000367 DASWBY010000000
VERSION     DASWBY010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578345"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VF870_06745"
     CDS             complement(<1..1055)
                     /locus_tag="VF870_06745"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEX9251922.1"
                     /translation="MSFGTISKIIFYLLLIFFVNPQFSFPQENPYRKVNKTYYPDGTI
                     KTEGDYLYDQLDGHYKEFYPNGNLWKDWKYVNGREEGISIWYFPDGKVSIEWNYIYGK
                     KEGVSRWYYETGELWSEQQYSNGVLQGITYTYYKTGEKQGEWNYVDGVLQGISKTFYT
                     NGNVEVERKFVDGRQHGTTKVYHDDKSLALEAFFIDNKLQGDVLIYDKMKTLRVRDTY
                     INDELINRVKFDYAGKVESKEQLVKNYYPSGRLADETYMVDGLKDGPMKTFYESGFLK
                     EEWNLKKNNIAGRSFEYYENGVLAVSSDYVDGVKRGNEKSYYENGILKAETIYENNLK
                     NGICLTYHPNGNLESIG"
     gene            complement(1137..2150)
                     /gene="aroF"
                     /locus_tag="VF870_06750"
     CDS             complement(1137..2150)
                     /gene="aroF"
                     /locus_tag="VF870_06750"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX9251923.1"
                     /translation="MIVVLEKTVNDKQLENIIKHLEDFGFAIHKSTGEERMILGAIGV
                     QPNFDTRKIKILDGVEEVYRITESFKLASRSFKKDDTIIKIKDVVIGGNEVSVIAGPC
                     SVESEEQIMIIAELVKKNQVKILRGGAFKPRSSPYSFQGLGEEGLKLLRKAADEFGLL
                     VITEVLENSMIDLVYKYTDIFQVGARNMQNYSLLKELGTAKKPVMLKRGLSATVEDWL
                     MSAEYILSNGNPEVFLCERGIRTFETYTRNTFDISAIPVVHKRSHLPVFADPSHATGL
                     RDKVIPMARAAVAAGADGLMIEVHHDPEKALSDGPQALLPEQFSDMMKQIKLIAEVIG
                     RKM"
     gene            complement(2164..>2416)
                     /locus_tag="VF870_06755"
     CDS             complement(2164..>2416)
                     /locus_tag="VF870_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX9251924.1"
                     /translation="GPQGQSYYSIWFTDKTTGKETYHVGRYLDFELNDDPEFVYELDF
                     NRAYNPYCATVIFSPVLFQQKTIILILKLKPARKIFILN"
BASE COUNT          727 a          489 c          348 g          852 t
ORIGIN      
        1 ttacctatgc tttcaaggtt gccattaggg tgatatgtta agcaaatacc gtttttaaga
       61 ttattctcat aaattgtttc agccttaaga atcccatttt cgtaatatga tttttcattg
      121 cctctcttta caccatcaac ataatcagat gaaactgcta aaacaccgtt ttcataatac
      181 tcaaaagatc tacctgcgat attatttttt tttaagttcc attcttcttt tagaaatccg
      241 ctctcataaa atgttttcat cggaccatct tttaatccat caaccatata agtttcatca
      301 gctaatcttc cggaaggata atagtttttt acaagctgtt ctttggattc aactttaccg
      361 gcataatcaa atttaacacg atttataagt tcgtcattta tatatgtatc acgtactcga
      421 agtgttttca tcttgtcata tattaacaca tcaccctgca atttattatc tataaaaaaa
      481 gcttcgagcg ctaaggattt atcatcgtga taaacttttg tagtaccgtg ctgcctgcca
      541 tctacaaact ttctctcaac ttcaacattc ccattagtat aaaaagtttt tgaaatgccc
      601 tgaagaactc catccacata attccattca ccctgctttt caccggtttt ataataagta
      661 taagttattc cctgcaaaac tccattgctg tattgctgct ccgaccaaag ttctccggtt
      721 tcataatacc atctggatac tccttccttt tttccataga tataattcca ttcaatactg
      781 actttaccat caggaaagta ccagatagat attccctctt ctcttccgtt tacatatttc
      841 caatctttcc agagatttcc atttggataa aattctttat agtgtccgtc aagctgatcg
      901 tataaataat caccttcagt tttgattgtc ccatctggat aataagtctt atttactttt
      961 ctgtaaggat tttcctgcgg gaaagaaaat tgagggttta caaagaatat tagtaaaaga
     1021 taaaatataa ttttagaaat tgttccgaag ctcatttatt cctgataata attttgaggc
     1081 aaagataaag cattttgatt aaaactaaac atccaaaaag aggtaagcaa atttgattac
     1141 atttttctcc cgataacttc agcaatcaat tttatttgtt tcatcatatc agagaattgc
     1201 tctggcaata atgcttgtgg tccatctgat aaagcttttt caggatcgtg atgtacttct
     1261 atcattaatc catcagcacc ggcagcaaca gcagcacgag ccattgggat caccttatct
     1321 cttaatcctg ttgcgtgaga tggatcagca aacacaggta aatggctgcg tttgtgaaca
     1381 accggaattg cagaaatatc gaaagtgttt ctggtgtaag tttcaaaagt tcttattcct
     1441 ctttcacaaa gaaaaacttc cggatttccg ttcgataaaa tatattcggc agacattaac
     1501 cagtcttcaa cagttgcaga taatccgcgt tttaacatta ccggtttttt agcagtacct
     1561 agttctttga gtaaagaata gttctgcata tttctagcac cgacctgaaa aatatcagtg
     1621 tacttataaa ctaaatctat cattgaattt tccagcactt cagttataac cagaagcccg
     1681 aactcatctg cggctttacg taaaagtttt aatccttctt cacccagacc ttgaaaagag
     1741 tatggtgaac ttctaggttt aaatgcaccg ccgcgtaaaa ttttcacttg attcttttta
     1801 acaagttcag caataatcat tatctgctct tcactctcaa cagagcaagg gccggcaatt
     1861 acactaactt cgtttccacc gataacgaca tctttaattt ttattatcgt atcatctttt
     1921 ttaaaactgc ggcttgcaag tttaaatgat tcggtaatcc tgtaaacttc ttcaacaccg
     1981 tcaagtattt ttatttttcg tgtatcaaag tttggctgca caccaattgc accaaggatc
     2041 attcgttctt cgcctgtaga tttatgaatt gcaaatccaa aatcttcaag atgcttaata
     2101 atattttcga gttgtttgtc atttactgtt ttttcaagaa caactatcac tttgtctcct
     2161 tgtttaattc agtatgaaaa tttttctcgc cggctttaat ttcaaaatca agataatcgt
     2221 cttttgttgg aacaggacag gtgaaaagat cactgtagca caatatggat tgtacgctct
     2281 gttaaaatct aattcataaa caaattcggg atcgtcattt aattcaaagt ctagatacct
     2341 gccaacatga taagtttctt ttcctgtagt tttatctgta aaccaaatgc tgtaataact
     2401 ttgtccttgc ggtcca
//
LOCUS       DASWBY010000368         5638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_240172, whole genome shotgun sequence.
ACCESSION   DASWBY010000368 DASWBY010000000
VERSION     DASWBY010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240172"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF870_06760"
     CDS             <1..442
                     /locus_tag="VF870_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9251925.1"
                     /translation="FSEEIEHFRFNTAIASLMELINELKVLENCSKEIQSYVLQRFAS
                     MLAPVAPHLGEECWQIIGNEKSIFQNPVVFEIDNEALIEETVNIAIQVNGKLRATIPV
                     PMNSEQDAVKPLVFADEKVNKFTDGKTIVKEIFVKNKIYNIVVK"
     gene            455..1732
                     /gene="serS"
                     /locus_tag="VF870_06765"
     CDS             455..1732
                     /gene="serS"
                     /locus_tag="VF870_06765"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX9251926.1"
                     /translation="MLDIKLIRDNPEKVKQGIANKNEKNRVDELLELDKQRREIIAHA
                     DELKSKRNSVSQQVGQMKKSGQDATEIIAEMKSVGDEIAEYDTKLRTIEEEIDVILRF
                     IPNLPHSSVPVGKTAEENVEVRLWVPDGFKFENDFKVMDHVELGKKHNILDFERGAKI
                     SGSGFPVYKGKGATLERSLINFMLDYHLQNHNYSEIFPPFLVNKESMRGTGQLPKMEE
                     DMYHIEKDGLYPIPTAEVPITNIHRDEILNESDLPIKYVGYSACFRREAGSYGKDSKG
                     FLRVHQFNKVEMVKFVKPETSYDELELLVKDAEDILQALNIPYRILMLCSGDLSFSAA
                     KCYDIETWSPAENKWLEASSCSNFEDFQGRRANIRFRNEQTKKPEFVHTLNGSGLATS
                     RLMVSLLENYQTPEGKIIVPKVLHKYTGFNVID"
     gene            1943..3691
                     /locus_tag="VF870_06770"
     CDS             1943..3691
                     /locus_tag="VF870_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9251927.1"
                     /translation="MKNLLTLKKYFIKYKTKLYWGMLFILISNSMTVYLPILLKDSIN
                     ELQKNTTSQILLRYGLLIVGTSIIAGIFRYLIRQTIIVVSREIEYDIRGNFWEHIQKL
                     PLRYFQNNSTGNIMSHATNDINAVRMFVGPAVMYSIDTSIRLIIVVAIMVSINLPLTI
                     YALLPLPILSYGVYRVGKLIHEKYTKIQENFSLLTTRAQENFSGIRVIKSYVREANEI
                     VRWNGLSKDYLQKNMNLVRIQAIIMPILYMITGISIIVVIWIGGGKVISGEMNLGDIT
                     AFIVYLGILIWPMIAFGWVMNIIQQGEASMKRLNKIFDEPYEIQDLSIGGTSVNELNG
                     KIEFKNVSFKYSDSLPEVLKNINLKINVGSTLAIVGHTGSGKTSLINLIPRLYDVTSG
                     EVLIDGNNIKDIPLDVLRTNIAVVQQESFLFSDSVYGNISYGLRQVDKQRVEEVSRIA
                     NFDKDVNSFPNGYETIVGERGITFSGGQKQRASLARALAVDPKILILDDSFSAVDTHT
                     EEEILKNLKEFMKDRTSIIISHRISTVKDADKIVVLENGRIAEEGTHEELVLLNGLYA
                     ELHFKQLLEKELEELN"
     gene            3739..5529
                     /locus_tag="VF870_06775"
     CDS             3739..5529
                     /locus_tag="VF870_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9251928.1"
                     /translation="MKDNQQDDEILGKAYDAKLMKRLLTYIKPYKKYVIFAILLNILV
                     AAFGPVRPYLTKIAIDDYIVNSNYNGLILIGVILFATLILQAVVQYFLTYYTQYLGQR
                     TLYDIRTQIFEHTQKLALKFFDRTPIGRIVTRTTNDVESLSELFSSGIVMVFSDVFII
                     LWILGFMFFMDIPLSLVTLSVLPVLIYGTFLFRKKVRESYRDVRFHLARLNSYMQEHV
                     TGMSVVQIFRKEKDELKQFSSINADHREANIKSIFYYAIFFPSVELLSSIAIALIVWY
                     GGGEIIQKSLTIGVLFAFIQYTEMFFRPIRDLSEKYNILQTSMASSERIFKLLDNQTF
                     INNPENALRLNSIKGEIQFKDVWFAYENDNYVLKNINFSINPGETVAIVGHTGAGKST
                     LINILTRFYDINKGSVLLDGFDIRKVDKRDLRKYISIVLQDVYLFSGTIKSNINMDND
                     EIGIEKIIDSAKTVGADKFISNLPNKYDEEVKERGATLSVGQKQLISFARALAYDPKI
                     LILDEATSSIDTETEQLIQSAIEKLLIGRTAIVIAHRLSTIQNADKIIVMHKGEIREI
                     GNHQELLAKRGIYYKLYQLQYKDQELKKSS"
BASE COUNT         1955 a          878 c         1051 g         1754 t
ORIGIN      
        1 gtttagtgaa gaaattgagc atttcagatt taatactgca attgcttcat taatggaatt
       61 gataaacgag cttaaagttt tagaaaactg cagtaaagaa attcagagtt atgttttgca
      121 aagatttgct tcgatgttgg ctccagttgc accgcatctt ggtgaagaat gctggcagat
      181 aatcgggaat gaaaaatcta tcttccagaa tcctgttgtg tttgagattg acaatgaagc
      241 tttgatcgaa gagactgtaa atattgctat tcaggtgaat gggaaacttc gcgcaactat
      301 tccggttcct atgaacagcg aacaagatgc agttaagccg cttgtttttg cagatgagaa
      361 agtaaacaag tttaccgacg gcaaaacaat tgttaaagaa atatttgtaa aaaataaaat
      421 ttataacata gtagtaaagt aattagggac ttaaatgtta gatataaaac ttattcgtga
      481 taatcctgaa aaagtaaaac aagggattgc aaacaagaat gaaaaaaatc gtgttgatga
      541 acttcttgag cttgataaac agcgtagaga aatcatcgct cacgctgatg aattaaaatc
      601 aaaacgaaac tcagtttcgc aacaagttgg gcagatgaaa aaatcaggac aggatgccac
      661 agaaataatc gcagaaatga aaagtgttgg tgatgaaatt gcagagtacg acacaaaact
      721 cagaacaatt gaagaagaaa tcgatgtaat tttaagattt attccaaatc ttcctcactc
      781 atctgtgcca gtaggaaaaa cagcagaaga aaatgttgaa gtaagactct gggttcctga
      841 tggctttaaa tttgaaaatg attttaaagt tatggatcac gtagagcttg ggaaaaaaca
      901 taatattttg gattttgaaa gaggcgctaa aataagcggc tccggcttcc ctgtttataa
      961 aggaaaaggt gcaacccttg agcgttcatt aatcaacttt atgcttgatt atcatttaca
     1021 aaatcataat tactcagaaa tttttccacc ttttctcgta aacaaagaat ctatgagagg
     1081 aaccggccag cttccaaaaa tggaagaaga tatgtaccac attgaaaaag atggtttgta
     1141 tccaattcca actgcggaag tgccaataac taatattcac cgtgatgaga tacttaatga
     1201 aagtgattta ccaataaaat atgttggata ttcagcctgc tttagaagag aggctggttc
     1261 ttatggtaaa gattcaaaag gatttttacg ggtacatcaa ttcaacaaag ttgaaatggt
     1321 gaagtttgta aaacccgaaa catcttacga tgaacttgaa ttacttgtta aggatgccga
     1381 agatattttg caggcactaa acattccata tagaatttta atgttgtgtt caggagattt
     1441 aagtttttct gcagcaaagt gttatgatat cgaaacctgg tcccctgctg aaaataaatg
     1501 gttggaagcg tcctcttgca gtaactttga ggattttcag ggacgcagag caaatattcg
     1561 tttccgtaat gagcaaacca aaaaaccgga atttgttcac acactaaacg gcagcggttt
     1621 agccacaagc agattgatgg tttctcttct ggaaaattat caaacaccag aaggaaaaat
     1681 tattgtgcct aaagtgctgc acaagtacac cgggtttaat gtgattgatt aaagacaggt
     1741 tgaaccagca ataccgtttg aaaggctcgt tttatcattt tacgggtaca cggaagatcc
     1801 cgaaacaagt tcgggatgac agaattatta aatgattcat atcaccctga aatagcttca
     1861 gagtttttat attcgcagag cgattgagca ataaaaaaat tatcttactc taaatcaaaa
     1921 tcttacagat acacttttat taatgaaaaa ccttttaaca cttaaaaaat attttattaa
     1981 gtataaaacc aaattgtatt ggggaatgct gtttattcta atttccaatt caatgactgt
     2041 ttaccttccg attttgttaa aagattctat aaatgaactt cagaaaaata caacttcaca
     2101 aatactttta aggtacggac ttctaatagt tggtacttca ataattgcgg gaattttcag
     2161 atacttaatc cgacaaacca taattgtagt atcacgtgag atagaatacg atattcgcgg
     2221 taacttctgg gaacacattc aaaaacttcc attaagatat ttccagaata actccaccgg
     2281 caatataatg tcgcatgcta caaatgatat caacgctgta agaatgttcg ttggtcctgc
     2341 tgttatgtac tcgattgata cttcaataag attgataata gttgttgcga taatggtatc
     2401 aataaatctt ccactaacaa tctatgcttt gttgccattg ccaattttgt cttacggcgt
     2461 ttatagagtt ggaaaactta tccatgagaa atacacaaag attcaggaaa atttttcact
     2521 gctaactacg cgcgcacaag aaaatttttc cggtatccgc gttattaaat cctacgtgcg
     2581 tgaagcaaac gaaattgtta gatggaatgg actaagcaaa gattacttgc aaaaaaacat
     2641 gaatcttgtt cgtattcagg ctatcattat gccgatttta tatatgataa ccggaatttc
     2701 tatcattgtc gttatttgga tcggcggtgg aaaagtaatt agcggagaaa tgaatcttgg
     2761 agatattacc gcttttatag tttatctcgg cattttaatt tggcctatga ttgcctttgg
     2821 ttgggttatg aacatcatcc agcaaggtga agcaagcatg aagagattaa ataaaatttt
     2881 tgacgaacct tacgagattc aggatttatc aatcggtgga acttcagtaa acgagttaaa
     2941 tggaaaaatc gaatttaaaa acgtttcctt taaatattca gattcacttc ctgaagtttt
     3001 aaagaatatt aatcttaaaa ttaatgtggg ctcaacactt gcgattgttg gacacactgg
     3061 ttcaggtaaa accagtttga taaatttaat tccaagatta tacgatgtca cttccggtga
     3121 ggttttaata gatggaaata acataaaaga tattccgctt gatgttttaa gaaccaatat
     3181 tgcggttgtt caacaggaat catttttatt ttctgattct gtttacggaa atatttccta
     3241 tggattacgc caggtagaca aacagagagt tgaagaagtt tcacgtattg caaactttga
     3301 caaagacgtt aattcatttc caaatggata tgaaacaatt gttggtgaaa gaggaattac
     3361 tttttcaggc ggtcaaaaac aaagagcttc acttgcgcgt gctctggctg ttgatcccaa
     3421 aatattaatt cttgatgatt cattttccgc tgttgataca catactgaag aggaaatcct
     3481 aaaaaatcta aaagaattta tgaaagacag aacaagtata atcatcagcc atagaatatc
     3541 gactgttaaa gatgctgata aaattgttgt tcttgaaaat ggcagaattg ctgaagaagg
     3601 aacgcacgaa gaattagtat tattaaacgg actttatgcg gaattacatt ttaaacaatt
     3661 gttagaaaaa gaattggaag agttgaatta gaaagagcct catctctccc taagaagagg
     3721 gtccaggaaa attattttat gaaagataac cagcaagacg acgaaatatt aggcaaagca
     3781 tacgacgcta agttgatgaa aaggcttctt acttacatta agccttacaa gaagtacgtt
     3841 atttttgcaa tattgttaaa catacttgtt gctgcatttg gacctgtaag accatatctg
     3901 acaaaaattg caattgatga ttacattgtt aattccaact ataacggact cattttaatc
     3961 ggcgtaatac tttttgccac acttattctt caggctgtag ttcagtactt ccttacttac
     4021 tatacacagt atcttggaca aagaacttta tacgatattc gtactcaaat ttttgagcat
     4081 actcaaaaac ttgcattaaa gttttttgat cgtacgccaa ttggaagaat tgtaacccgc
     4141 accacaaatg atgttgaatc gttaagtgaa ttattttcgt ccggaattgt aatggtgttt
     4201 agcgatgtgt ttattattct atggatactt gggtttatgt tctttatgga tattccgctt
     4261 tcacttgtta cactttctgt gcttccggtt ttaatctatg gtacattttt attcaggaaa
     4321 aaagtaagag aaagttatcg cgatgtaaga tttcatctgg caagattaaa ttcctacatg
     4381 caggaacacg taaccggaat gagtgttgtc cagatattca gaaaagaaaa agatgaactt
     4441 aaacagtttt caagcatcaa tgccgatcat cgtgaagcaa atataaaatc aatcttttat
     4501 tacgctatct ttttcccgag cgttgagctg ctcagctcaa ttgcaattgc tctaattgtc
     4561 tggtacggtg gcggtgaaat tattcaaaaa tctttaacta tcggcgtgct ctttgccttt
     4621 attcaatata cagaaatgtt cttccgtccg attcgtgatt tatctgaaaa atataatatt
     4681 ctacagacat ctatggcttc atcagaaaga atttttaaac ttcttgataa tcagacattt
     4741 ataaataatc cggaaaatgc gttaagacta aattcgatta agggtgaaat tcaatttaaa
     4801 gatgtttggt ttgcttatga gaatgataat tacgttttga agaatattaa ttttagtatt
     4861 aaccctggag aaacagttgc aatcgttggt cataccggag ctggtaagtc aactttaata
     4921 aatatcctaa ctagatttta cgacatcaat aaaggttctg ttttgctcga tggttttgat
     4981 attcgtaaag ttgataagcg ggatttaaga aaatatattt caattgtttt acaggatgtt
     5041 tatctttttt ccggcactat caaatcgaat atcaatatgg ataatgatga gattggtatt
     5101 gaaaagataa ttgattctgc aaaaactgtt ggtgctgata aatttatttc taatctacca
     5161 aacaagtatg atgaagaagt aaaagaacgt ggggcaactt taagtgtggg acaaaaacaa
     5221 ttaatttctt ttgcaagagc tttagcttat gatccaaaaa tcttaatttt ggacgaagct
     5281 acttcaagta tagatactga aacagagcag ttgattcagt ctgcgattga aaaacttctt
     5341 atcggcagaa ctgcaattgt aattgcacac agactttcaa ctatacagaa tgcagataaa
     5401 attattgtta tgcacaaagg tgaaatcaga gagattggta atcatcagga gcttcttgca
     5461 aaacgaggta tttattacaa gttatatcaa ttgcagtaca aagatcagga attaaaaaaa
     5521 tccagctaac aaattttaga atgggagatt tgcaatggaa agaataaaat ttaatactct
     5581 cgcccgccat atttacgagg aagaaagaaa atatccagaa gctacaggcg aactgtct
//
LOCUS       DASWBY010000369         4903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_200580, whole genome shotgun sequence.
ACCESSION   DASWBY010000369 DASWBY010000000
VERSION     DASWBY010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200580"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..919)
                     /locus_tag="VF870_06780"
     CDS             complement(158..919)
                     /locus_tag="VF870_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9251929.1"
                     /translation="MLKEKGILFDVALCVGCGACYQACKETNNLAETNKDFLKDHLSD
                     NTFTVIEQYGELFTRKLCMHCNEPACVSVCLVGAITKSPTGLVVYDANKCIGCRYCMQ
                     ACPHKIPRYEWTSTQPRIRKCNLCTDRVKNGQLTACAEACPTEATLFGDMDQLIAVAK
                     KRLKDNPEKYYQHIYGLEEAGGGHVLVLSPVPFEQLGYMSKLPKESMPEFTMRAMEKI
                     PSVVVGGGVFLTGMYWLTKRKNQISKDEKNNSKRN"
     gene            complement(934..1605)
                     /locus_tag="VF870_06785"
     CDS             complement(934..1605)
                     /locus_tag="VF870_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251930.1"
                     /translation="MVAIVVLAMFLIFLSVDLIVLKIQGKYHPAFEPSFFQFNSSQVE
                     INNYPIPPDIFFSKGHTWLKKNNEGLISIGIDEFASSALGMISILNCADKGKQLKRGD
                     VIFEAAYGNTKVKFLSPINGLVKSVNSNLVGKRVSNPYEAWGVQIASDDSTDNKQVFY
                     SGKEAFDWIKKESIKLKDFIISHSLKPDLAGATMFDGGSLSNDAISLLVDNSVNDFEK
                     EFLSL"
     gene            complement(1627..1884)
                     /locus_tag="VF870_06790"
     CDS             complement(1627..1884)
                     /locus_tag="VF870_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251931.1"
                     /translation="MLTFLFILILVFVGVDLLVRFVIDPLASGSNKKHKKSKSYTPRF
                     DPTIKLASETMYDGGKPHDVKSSDESENADLSKDTSKKSEQ"
     gene            2524..3426
                     /locus_tag="VF870_06795"
     CDS             2524..3426
                     /locus_tag="VF870_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9251932.1"
                     /translation="MSGKSRRDFLKTSALIGAGVTGLSTTAKAGPKNILSEDRMGVLV
                     DTTVCVGCRNCEWACKDAHDLPAGNLVSYEDRKVLESKRRPDDKALTVVNEYSAGKNS
                     NLPVDVKVQCMHCDHPACVSACIVGAFSKHENGSVTWDTDMCIGCRYCMAACPFQVPA
                     FEYDKALNPKIMKCDFCIERTKEGKVPACVSICPVEALTYGSRTELVKIARDRIKRNP
                     DRYVDHIFGEYEVGGTSWMYLANKDFAELDFPNLGKNPAPGVSESIQHGIFAYFVPPV
                     SLYALLGGIMWISKRRKELDEEIK"
     gene            3427..4671
                     /gene="hybB"
                     /locus_tag="VF870_06800"
     CDS             3427..4671
                     /gene="hybB"
                     /locus_tag="VF870_06800"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HEX9251933.1"
                     /translation="MNNELHNPVPIKHKYFTPGVFALLILALNGLVFLMGRFFFGIGA
                     VTNLNNQYPWGLWIGVDVAAGVALAAGGFTTAALGHIMHKDEYHAIVRPALLTAMLGY
                     TFVAVGVFVDIGRWYFIWHPLIMWNGNSALFEVGICVMIYVTVLYIEFLPVVTERFIG
                     KVNLPGFFSGLNKIVDKFLRLLDKGLSKTMFIFIIAGVVLSTLHQSSLGTLMIIAGPK
                     MHPLWQTPILPLLFLLSAISVGFPMVIFESLLASRSLGLKPEMHILSKLGSMIAPLLG
                     IYLAFKIGDMFIRKTFVYLTDFNTASVMFTIEMLFGVIIPLRMFLSTKVLKSPLFLFI
                     ASALVVFGVLINRINNFVVAYTPPYSTHSYFPSVGEISVTVGFVAILVLIYRFIVINF
                     PVISLPGKQTAPQSKYTIRGGK"
     gene            4671..>4903
                     /locus_tag="VF870_06805"
     CDS             4671..>4903
                     /locus_tag="VF870_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251934.1"
                     /translation="MKKIFILLTVLFFAAAMSAQQSPGKDHAKLQINCKTCHTCDVPT
                     KSEPCLVLCPREKMTTVYQKPEQTPELIILDQI"
BASE COUNT         1528 a          856 c          847 g         1672 t
ORIGIN      
        1 atccataaac cccagggaac agtatccttc agatttgttg ttgccccgag tccgagtgaa
       61 aatcttaaat atgtggaaat caatcccatt gtaacaatga taaccgaaac aactttccaa
      121 aatgatggtt ttaaaaattc tcttattttc ataatctcta atttctttta ctattatttt
      181 tttcgtcttt ggatatttga ttttttcttt tagttagcca gtacatcccg gttagaaaaa
      241 ctccaccacc cactactacc gacggaatct tttccattgc ccgcattgta aattcaggca
      301 ttgattcttt aggtagtttg gacatataac ctagctgttc gaatggtact ggagataaaa
      361 ccagaacatg accaccgcct gcttcttcaa gtccgtatat atgttgataa tatttctccg
      421 gattatcttt taaccttttt ttagccacag caataagttg atccatatct ccgaataatg
      481 ttgcttctgt tggacaagct tcggcacaag cagttagttg accatttttt acccggtcag
      541 tacaaagatt gcatttgcgt atacgtggtt gggtactagt ccattcataa cgtggaattt
      601 tgtgaggaca agcctgcata caatatctgc acccaataca tttatttgca tcataaacaa
      661 ctaagcctgt aggacttttt gttattgctc caacaagaca gactgatacg caagccggtt
      721 cattacaatg catacaaagt tttcttgtaa ataattctcc atattgttca ataaccgtaa
      781 aagtattgtc tgagagatgg tcctttaaaa aatctttatt tgtttccgct aaattatttg
      841 tctctttgca agcttgatag caggcaccac atcctacaca caacgctaca tcaaagagga
      901 tccctttttc cttcaacatt tatttcccaa ttattataat gatagaaatt ctttttcaaa
      961 atcatttaca ctattgtcaa ctaataaaga gatcgcatca ttggataacg aacctccatc
     1021 aaacatagtt gcaccagcta aatctggttt gagtgaatga ctaatgataa agtcttttaa
     1081 ttttatagat tcttttttta tccagtcgaa tgcctcttta ccagaataaa acacctgttt
     1141 attatctgtt gaatcatctg aggctatctg aactccccag gcttcatatg gattggaaac
     1201 tctttttcca accaaattac tgttaactga ttttactaat ccgtttatgg gagacaaaaa
     1261 ctttaccttt gtatttccat atgccgcttc gaatatcaca tctcctcgtt ttaattgttt
     1321 gcctttatca gcacaattta aaattgaaat cattcctaaa gctgaagatg caaattcatc
     1381 tatgccgatt gaaattaatc cttcgttatt ttttttaagc caggtatgac cttttgaaaa
     1441 aaatatatct gggggaatcg gataattatt tatctcaacc tgtgatgaat taaattgaaa
     1501 gaacgacggt tcgaatgcag gatggtattt cccctgaatt tttaatacaa taagatcaac
     1561 tgaaaggaaa attaaaaaca tagccagaac aacaattgca accataataa tgtatcccta
     1621 tataaattat tgttctgatt ttttacttgt gtcttttgaa agatcggcgt tttcactttc
     1681 gtcgcttgat tttacgtcat gcggttttcc gccatcgtac attgtctcac ttgcaagttt
     1741 aattgtcgga tcaaatcttg gtgtgtaaga ttttgatttt ttgtgtttct tatttgatcc
     1801 tgaagctaat gggtcgatta caaatcgaac gagtaaatcg acaccgacaa agactaagat
     1861 aagtatgaat aaaaacgtaa gcatgatgaa ctccttttaa ctgttgattg tgttattgga
     1921 tgggcaatta aaataccagt aaatctcatt tttttattgg acatttatct tattaaaaaa
     1981 taatgtgtta tagagatgat gttggaaagt tatacgttct ggatttataa aagtgtaagt
     2041 atcctcaaca acacagtgtt gaataataca tcataatttt atagatgcag tgttgttaaa
     2101 tactacaaat ctatttgttc aagtcacaca aattttgatt gatcaattac ttgtttaaga
     2161 tccgaagagg ggcaaaaaca atttattttt ttagatagat gatcttgaga attaaagact
     2221 ggaagctatt aaaaatatac ttagtaatta caaacagtac acattcatca agcattggca
     2281 actaacaatt tattctctat cataaaaatt tttttttatt acttatcatc aatgagaatt
     2341 actttgatat caataacccc aaaaatttac ttcaccagtt gcaactcttg atgaattcaa
     2401 taatttagga cgattttcat ttctttaaat ttttgcaaaa aatggtacga tattgtattg
     2461 caaaaacagt taacaaaata taatgcaata ttaattcgta acatcttttg aaaggagggg
     2521 aaaatgtctg gcaaaagccg tcgcgatttc ctcaaaacat ctgctttaat tggtgctgga
     2581 gtcacaggac tttcgacaac tgcaaaagca ggtcccaaaa atattttatc tgaagacaga
     2641 atgggggttc tagttgatac tactgtttgt gtcggctgta gaaactgtga atgggcttgt
     2701 aaagacgctc atgatctgcc cgccggaaat ttggtttcat atgaagatag aaaagtttta
     2761 gaatcaaaac ggagacccga tgataaagct cttacggtag ttaatgaata ttctgccgga
     2821 aaaaattcta atcttcctgt agatgtaaaa gttcaatgta tgcattgtga tcatcctgcc
     2881 tgtgtttctg cctgcatagt cggagcattt agcaaacacg agaatggttc agttacctgg
     2941 gatacagata tgtgcattgg ttgccgttac tgtatggcag cttgtccatt tcaggtccca
     3001 gcatttgaat atgataaagc tctcaatcca aagattatga aatgtgattt ttgtattgaa
     3061 agaactaaag aaggaaaagt tccagcttgc gtcagcattt gtcctgttga agcactaacc
     3121 tatggaagca gaacagagct tgtaaaaatt gcacgcgata gaattaaaag aaatccagat
     3181 agatatgttg atcatatatt tggagaatat gaagttggcg gtacatcctg gatgtatctt
     3241 gcaaataaag attttgcaga actcgatttt ccaaatcttg gtaaaaatcc cgcacccgga
     3301 gtttcggaat caatacaaca cggaatcttt gcatactttg ttccgcccgt ttcgttatac
     3361 gcattgcttg gcggtattat gtggatttcc aaacgacgca aagaattaga tgaggagata
     3421 aaataaatga ataacgaatt acataatccg gtaccgatta aacataaata ttttactccg
     3481 ggagtttttg cattattaat tcttgcatta aacggtttag tttttttgat gggcagattt
     3541 ttctttggaa ttggcgcagt tacaaattta aataatcaat acccatgggg attatggatc
     3601 ggggttgatg ttgctgcggg tgttgcactt gccgccggtg gatttactac ggcagcactt
     3661 gggcatatta tgcacaaaga tgaatatcac gcaatagtac gccccgcatt attaactgca
     3721 atgcttggat atacttttgt tgcagtaggc gtttttgttg atataggccg atggtatttt
     3781 atctggcatc cattaataat gtggaatggt aattcagctt tatttgaagt tggtatttgc
     3841 gttatgattt atgttactgt tctttacatc gaatttcttc ctgtagttac ggaaagattt
     3901 atcggcaaag ttaatttacc aggattcttt tcaggactta ataaaattgt tgataaattt
     3961 ctgcgccttc ttgataaagg actttcaaaa acaatgttca tctttataat tgcgggagtt
     4021 gtactttcaa cacttcatca atcttcctta ggaactttga tgataatagc agggcctaag
     4081 atgcatccat tgtggcaaac cccaatctta ccgcttttat ttttgctttc agctatctct
     4141 gttggatttc caatggtaat ttttgagtcg ttgctagcct cgcgttcatt ggggctaaaa
     4201 ccagaaatgc atattctatc taaattagga tcgatgattg caccactttt aggaatttat
     4261 cttgcattta aaattggtga tatgtttata agaaaaacat ttgtttattt aaccgacttt
     4321 aatacagcaa gcgtaatgtt tacaattgaa atgctgtttg gagttataat accgctgcga
     4381 atgtttcttt ctaccaaagt attaaaatct cctttattcc tttttattgc atctgcctta
     4441 gttgtatttg gtgtacttat aaatcgaatt aataattttg tggttgctta cactccacct
     4501 tacagcacac attcatactt cccatcggta ggtgaaattt ctgtaactgt aggatttgta
     4561 gcaatactgg tactcatata cagatttatc gtaataaatt ttccggttat cagtttgccg
     4621 ggtaaacaaa ctgctcctca atccaaatac acaataagag gaggaaaata atgaaaaaga
     4681 tatttatact actaacagta ttgttttttg cagctgcaat gagtgcacag caaagtcctg
     4741 gtaaagatca tgcaaagctt caaattaatt gtaaaacatg tcatacgtgc gatgttccaa
     4801 caaaaagtga accttgcctt gttctttgtc caagagaaaa aatgacaacg gtttatcaaa
     4861 aaccagaaca aactccggaa ctgattattt tagatcaaat aag
//
LOCUS       DASWBY010000370         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_17331, whole genome shotgun sequence.
ACCESSION   DASWBY010000370 DASWBY010000000
VERSION     DASWBY010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17331"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..>2172)
                     /locus_tag="VF870_06810"
     CDS             complement(291..>2172)
                     /locus_tag="VF870_06810"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX9251935.1"
                     /translation="KFAASKTVKYILIICAIVFSLSGILLAASLYWTKLETLRLFNLW
                     QTIIRFTSVIIFVASFISLIVQYIKEKNPESKKPLLLFVIAFGFGMIIQLYIGTIAPA
                     ISDTVFNSPEYYLPIFLSVLVPITFAYAIFKYHLLDVSIVVRNTIIYGAAMATVAGIY
                     FLIIYLLGQGIGSFVGEESQGFVAGIFFLIFAFVFQSTKDRFQDFLTRKFYPEQFAYQ
                     KVLIQFSNDVSTVVGTDKILDLTSETFIDALKINKFGILLRDYKKDVLSLARGVGFSN
                     PNCVIEKSNLINVFKEKALISNTPVIEQQDFSNVFPDKAHRLIEEGVYSIIPMIVKSK
                     VVGALLFGLKYSGSKFGGKDLELLNAAANQIAISLENARLYQSESEKIKIERDLDLAR
                     KIQQGLLPKCIPNLSGLDICGEMIPAMQVGGDYYDLIQLSGTKLFVAVGDVSGKGLSA
                     SLYMTKLQTMIQISTKASASPKEILIEINKRLYESIERSWFITMTLALFDTEAKTVKF
                     CRAGHMPLLTANNGTVESIKTEGIGLGLEKGEVFDRTLQEVEIKIKPNQIFAFFSDGI
                     TEAMNENDELFGEDKLNEILKGKSYNRSSEIMNKVWSEVNNFRGSAQPNDDMTMVIVK
                     VS"
BASE COUNT          761 a          388 c          313 g          710 t
ORIGIN      
        1 cattcccgga aatcaatacc tgtaattacc ccattactca taaacatatt ttcatcttct
       61 gtacaggata atatgccgat gattaaaagt attccgacaa atattgtttt tttcatataa
      121 tcaccatttt taattatagg aaagatatga ggataaaatt aaaatgcaat gtacaagata
      181 aggcttagtg atgagttgaa aaggaattat tattatataa tagatattta aatgattaaa
      241 tactcaaaga aaaaattgat attacatgta aaaatattaa agaaccagta ttaactaact
      301 ttcacaatta ccatagtcat atcatcatta ggctgggcag aaccacggaa attgtttact
      361 tcactccaaa ctttattcat aatctcagat gaacgattat atgatttgcc ttttaatatt
      421 tcgtttagtt tatcttcacc aaacaattca tcattttcat tcattgcttc tgtaattcca
      481 tctgagaaaa atgcaaaaat ctggtttggt tttattttga tttcaacttc ctgtaaagtt
      541 ctgtcaaata cttctccttt ttctaaaccc aatcctattc cctctgtttt tatcgattca
      601 actgttccat tgtttgcagt aagcaaaggc atatgccctg cccggcaaaa ctttacagtt
      661 tttgcttcag tatcaaataa agctaaagtc atcgttataa accaacttcg ctcaatggat
      721 tcgtataagc gtttattaat ttcaattaat atttctttcg gacttgcact tgctttagta
      781 gaaatctgaa tcattgtttg cagtttagtc atatataatg aagctgagag ccccttacct
      841 gaaacatcgc caactgcaac aaataacttt gtacctgaaa gttgaataag atcataataa
      901 tcaccgccaa cttgcatagc aggaatcatc tcgccgcaaa tatccaatcc gcttaagtta
      961 ggaatgcatt taggcagtaa accttgctgt attttcctgg ccagatcaag gtctctttca
     1021 atcttaattt tctcagattc tgattgatat agtctggcgt tttccaatga gatagcaatt
     1081 tgatttgcgg cagcatttaa tagctcaaga tctttgccgc caaactttga tcccgaatat
     1141 tttaatccaa ataatagtgc acctacaact tttgatttaa cgatcatggg tattatggaa
     1201 taaacgcctt cttcaattaa tctatgagct ttatcaggaa aaacattact aaaatcctgc
     1261 tgttcgatta ccggtgtatt ggaaatcaat gctttttctt taaatacatt aatgagattg
     1321 gatttttcaa ttacacaatt tggatttgag aatccaactc cacgagctag agacaggaca
     1381 tcctttttat aatctcttaa aagaattcca aatttgttaa tcttaagtgc atcaataaat
     1441 gtctcgctgg tcagatcaag tattttatct gtgcctacta ccgttgatac atcattgctg
     1501 aattggataa gaactttctg atatgcaaac tgttcaggat aaaattttct tgtaagaaaa
     1561 tcctggaaac gatctttagt tgattgaaat acaaaagcaa aaatcaaaaa gaaaattccc
     1621 gcaacaaacc cctgactttc ctcaccaaca aagctgccta tcccctgccc aagtaaatag
     1681 attatgagaa aatagatacc agcaactgta gccattgcgg caccataaat aattgtattc
     1741 ctaacaacaa tacttacatc aagcaaatga tatttgaaga ttgcatatgc aaaagttata
     1801 gggactaaaa cactcaaaaa aattggcaaa taatattcgg gagaattaaa aacagtatca
     1861 gaaattgccg gagcgattgt acctatatat aactggataa tcattccaaa accaaaagca
     1921 atcacaaaaa gcagaagggg ctttttactt tcaggatttt tctcttttat atattgtaca
     1981 attagtgata tgaaagaagc aacaaaaata ataacagaag taaatcttat aatggtttgc
     2041 caaagattaa ataatctgag tgtttctaac tttgtccaat acaatgatgc tgcgaggaga
     2101 attccggata aactaaaaac aattgcacaa ataattaaaa tatattttac agttttagag
     2161 gctgcaaatt ta
//
LOCUS       DASWBY010000371         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_502560, whole genome shotgun sequence.
ACCESSION   DASWBY010000371 DASWBY010000000
VERSION     DASWBY010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502560"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VF870_06815"
     CDS             <1..1225
                     /locus_tag="VF870_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251936.1"
                     /translation="PEIFVSDSSAYVADSLKLLEDYLAKKELQDIWKGVDNLNNKNPN
                     LKDSTFIKDSVFVRDSLVKIDSLINIGRYNASDTVGLKKRMLDSLKQVHIAELKNPKN
                     IINNPSTTIKLDSVKFKKNPPQKLRITVDSAKTVLAKNTLELGNLFLTDLDVPDSSYY
                     LYKKILDDYNSPVYYPNTLYALGSYYLTINEKEKADSLFEIIYNDYQNRSIVNAAADK
                     LNKPLIDLNFDPAKDQYASAEDLMLGGNYSQSLNKFLNIYREYPKSTIAPQALYTSGW
                     ILENDLFLLDSAASVYDTLIAKYPKSVYVKNVSKKITAYKQEKVRIQKAIDDSLKALK
                     KITLDSTVVAVNTDEDSLKLVQQVFGGVIENTKIDENANADDLNNEGIDPKQQTGVVP
                     KKKLEPLWDPRRHFN"
     gene            1257..>1590
                     /locus_tag="VF870_06820"
     CDS             1257..>1590
                     /locus_tag="VF870_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9251937.1"
                     /translation="MRYRELLNLIEEGENLQVEFKRKFSTHDKIAREMIAFANTKGGY
                     VLFGVDDDKKIVGVESEKETTELVKDAALNYCEPPLEFSIEYREIEGKEVVIVKIPES
                     IKKPHRIQD"
BASE COUNT          583 a          243 c          277 g          487 t
ORIGIN      
        1 tccagaaatc tttgtcagcg attcttcagc ctatgttgcc gattcattaa aacttctaga
       61 ggactatctt gcaaagaagg aactacagga tatttggaag ggtgttgata atttaaataa
      121 taaaaatccc aatcttaaag attccacttt tataaaagat tcagtttttg ttcgtgatag
      181 tttagttaag atagacagtt tgattaatat tggtcgctat aatgcatctg atactgttgg
      241 actcaaaaaa agaatgctgg attctttaaa gcaggtacat attgctgaac ttaaaaatcc
      301 caaaaatatt attaataatc cttcaactac aattaagctt gatagtgtta agtttaaaaa
      361 gaacccgcca caaaaactta gaataacggt tgactctgca aaaacagtac ttgcaaaaaa
      421 tacacttgag ttgggaaatt tatttctaac agacttagat gttccggatt catcatatta
      481 tctttataaa aaaatattag atgattataa ttctccggtc tattatccaa atactttata
      541 tgcgcttggc agctattatt taaccattaa tgaaaaagaa aaagcagata gtctgtttga
      601 aataatttat aatgattacc aaaacagatc gatcgtaaat gctgctgcgg ataaattaaa
      661 taaaccgctt attgatttga attttgatcc ggcaaaggat cagtatgctt cggctgaaga
      721 tttaatgcta ggtggaaatt actctcagtc attaaataaa ttcttaaaca tttaccgtga
      781 atatccaaag tcaactattg cacctcaggc attgtacact tcaggttgga ttcttgagaa
      841 tgatcttttc ttactggatt cagcagcttc agtttatgat actttaattg caaaatatcc
      901 caaaagtgtt tatgtgaaaa atgtttcaaa aaaaattaca gcttataaac aggaaaaagt
      961 aagaattcaa aaagcaattg atgatagcct gaaagctctc aaaaaaatca ccctggattc
     1021 cacagttgtt gcggtaaaca ctgatgaaga ttcacttaaa cttgtgcagc aagtatttgg
     1081 cggtgttata gaaaatacta aaattgatga aaatgcgaat gctgatgatt tgaacaatga
     1141 aggaatagac ccgaaacaac agactggagt ggttcccaaa aagaaacttg aacctctctg
     1201 ggatccacgc agacatttca attaatttaa atagattaaa attaattcct tccgttatgc
     1261 gctacagaga attattaaat ctcattgaag aaggtgagaa tttacaagtt gagtttaaaa
     1321 gaaaattttc gactcacgat aagattgcac gtgaaatgat agcatttgca aacaccaaag
     1381 gcggctatgt tttatttggt gttgatgatg ataaaaagat tgtcggtgtt gaaagcgaaa
     1441 aagaaactac agaattggtt aaagatgctg ctcttaatta ttgcgaaccc ccgcttgaat
     1501 tttcaataga gtacagagaa attgaaggta aagaagttgt gattgttaag attcctgaat
     1561 caattaaaaa accacaccga atccaggact
//
LOCUS       DASWBY010000372         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_17406, whole genome shotgun sequence.
ACCESSION   DASWBY010000372 DASWBY010000000
VERSION     DASWBY010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17406"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VF870_06825"
     CDS             <1..695
                     /locus_tag="VF870_06825"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9251938.1"
                     /translation="LLKKVLIKRDIDVTLIHFLSSALRFALYLFVIIAAVGQLGVETT
                     SFIALLGAAGLAIGLALQGSLSNVAAGVMLIIFRQVKVGQFIEGAGVSGTVEKVGVFN
                     TTLLTIDNKVIYVPNSKLINDNIINYSEKDKRRIDLVVSISYSDDIDKAKKILFDLIN
                     SDSKILQDPEPVIAVGELADSSVNLFVRPWVNTPDVFEVRFRLIESIKKKFDEEQITI
                     PFPQRNVFIHNK"
     gene            711..>1845
                     /locus_tag="VF870_06830"
     CDS             711..>1845
                     /locus_tag="VF870_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX9251939.1"
                     /translation="MSSKESFIQLITDGYSFKGDSLILGTAIYNNEPQQNLHIKIPLS
                     TINRHGLIAGATGTGKTKTVQVFAEALAEKSIPVLLMDIKGDLSGIAAEGSSNPKIEE
                     RHAKIGIPYKPKNFSTEFLTLSDEKGVRLRATVSEFGPILISKILELNDTQAGLISVL
                     FKYCDDNNLALLDLKDLKKVLQFAAAEGKDSIEKDYGSISTSSLGTILRKVVELEQQD
                     ADKFFGEKSFEVNDLLRIDENGYGIISVLRLIDLQDRPKLFSTFMLALLAEIYSTFPE
                     LGDKAEPKLVIIIDEAHLIFNEASKALLNQIETIIKLIRSKGVGIYFCTQNPTDIPAS
                     VLSQLGLKIQHALRAFTAQDRKMIKLTSENYPISEYYKTDELLT"
BASE COUNT          625 a          295 c          343 g          582 t
ORIGIN      
        1 gacttctaaa aaaagtcctt ataaaaagag atatcgatgt aactcttatc cattttttat
       61 caagtgcttt acgatttgca ttatacttgt ttgtaattat tgcagctgtt ggtcagcttg
      121 gtgttgaaac tacttcattt attgctctat taggtgctgc aggacttgca attggtcttg
      181 cattacaggg ttctctttca aatgtagcgg ccggtgtaat gttgataatt ttcagacagg
      241 taaaagtcgg gcaatttatt gaaggagcag gtgttagcgg cacagtagaa aaggtaggtg
      301 tatttaatac gactttgctg actatagaca acaaagttat ttatgtccca aactccaagc
      361 tgattaatga taatattatc aattattcgg aaaaagataa acgaagaatt gatctggtcg
      421 tttctataag ctattctgat gatattgata aagctaaaaa aatattattt gatttaatta
      481 actccgattc aaagatactt caagatcccg aacctgtaat agcggtaggt gaacttgctg
      541 acagcagcgt taatctattt gtcaggccat gggtaaatac tcctgatgta tttgaagtaa
      601 gattcagatt aattgaatca attaagaaaa aatttgatga ggagcagatt actattcctt
      661 tcccgcaaag gaatgtcttt attcataata aatgatgatt ggaaaacatt atgagttcta
      721 aggaatcttt tatacaatta attaccgatg gatattcatt taaaggcgat tcattaattc
      781 tcggcactgc tatttataat aacgaacctc agcaaaatct tcatattaaa attccgctta
      841 gtacaataaa cagacacggt ttaattgcag gagctacagg aacgggtaaa acaaagaccg
      901 ttcaagtatt tgcagaagca ctagcagaaa aaagtattcc tgtattacta atggacataa
      961 aaggtgattt gagcggaata gccgccgaag gctcatccaa ccccaaaatt gaagaacgcc
     1021 acgctaagat tgggatacca tataagccta aaaatttttc gactgagttt ctaactctat
     1081 cagatgaaaa aggtgtgcga ttaagagcta cagtttctga gttcggcccc atcctgattt
     1141 caaaaatact tgaactaaat gacactcagg caggtttgat atcggtatta tttaaatatt
     1201 gcgatgacaa caatcttgcc ctgcttgatc ttaaagatct aaagaaagtt ttacagtttg
     1261 cagctgctga aggaaaagat tctattgaga aagattacgg ctcaatatca acttcttctt
     1321 taggaacaat actaagaaaa gttgtggaac tggaacagca ggatgctgat aaattttttg
     1381 gagaaaaatc ttttgaagtt aatgatcttt tacgaattga tgagaacgga tacggaataa
     1441 tttctgtctt aagactgatt gatctgcagg atcgtcctaa attgttttct acttttatgc
     1501 tggcactgct tgcagaaatt tattcaacat ttcctgagct tggagataaa gctgaaccta
     1561 aattagttat tataattgat gaagcacatt taatttttaa cgaagcttct aaggctttat
     1621 taaaccagat tgaaaccatc attaaactaa tccgatcgaa aggtgttggg atttattttt
     1681 gcacgcaaaa tccaacagac atacctgctt cagttttaag ccagcttggt ttaaaaatac
     1741 agcatgcact tagagcgttt actgcgcagg atagaaaaat gattaaacta acttcagaaa
     1801 attatccaat atcagaatat tataagacgg atgaattact gactt
//
LOCUS       DASWBY010000373         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_502680, whole genome shotgun sequence.
ACCESSION   DASWBY010000373 DASWBY010000000
VERSION     DASWBY010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502680"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VF870_06835"
     CDS             complement(<1..656)
                     /locus_tag="VF870_06835"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX9251940.1"
                     /translation="MKRREFIFKSALAGAALSAYQIFPFGVIKKVNSVGNPLRFPPEL
                     QPGESLVFKSANIEVWPGTITQILGLNNSYPAPTIRVQKGDNFSVLFENQHTEEATIH
                     WHGLLVPELMDGQPKDAILPGDSYTYSFPIFQNAGTYFYHSHAHHLTANQVYKGYAGF
                     FIVEDNAELQYGLPSGEYDIPLLIQDRHSVNQPQFTYAPTMMDKMTGYLGDVPLVNGT
                     P"
     gene            complement(709..>1708)
                     /locus_tag="VF870_06840"
     CDS             complement(709..>1708)
                     /locus_tag="VF870_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9251941.1"
                     /translation="ALSAKSCLLIRNRNAFEQARNIQAIIFDKTGTLTKGEFGITETI
                     SFDKNYGEKEILKYAASLESESEHPIAQGIVKSSEDKYELKEFNSIPGKGAEGKVNGK
                     DVKVVSPRYLEENKIESQEKDKIEKISAQGKTVVFVLIDNKLIGAIALADIIREESKE
                     TIKQLKELGIRPMMLTGDNKRVAKWVADELELEVYFAEVMPDKKAEKVKEVQSRGLVV
                     AMTGDGVNDAPALAQSDVGIAIGTGTDVAVETADIILVKSNPKDVVSLIKFAKATYSK
                     MIQNLIWATGYNVVAIPLAAGVLYNFGIVLSPALGAVLMSASTIIVAINAKFLRVN"
BASE COUNT          500 a          362 c          266 g          580 t
ORIGIN      
        1 ggtgttccgt taacgagagg aacatcaccg agatatccag tcattttgtc catcattgtt
       61 ggcgcgtaag tgaactgcgg ctgatttaca gaatgtctgt cctgaatcaa taatggaata
      121 tcatattctc cagatggaag accatattgc aactcagcat tgtcttcaac aataaaaaat
      181 ccagcataac ctttataaac ttgatttgcc gttaagtgat gtgcatgaga atgatagaaa
      241 tatgtacctg cattctgaaa aatcggaaat gagtaagtat agctgtcacc aggtagaatt
      301 gcatcttttg gttgcccatc cattaactcc ggtactagta atccgtgcca atgaatggtt
      361 gcttcttctg tatgttgatt ttcaaaaaga actgaaaaat tgtcaccttt ctgaacacga
      421 atagttggtg ctggatagga attatttaat cctaatatct gagttatagt acctggccaa
      481 acctcaatat ttgcagattt aaatactagt gactcaccag gttgcagttc tggtggaaag
      541 cgaagtggat taccaacact gtttactttt tttatcacac caaacggaaa aatttggtat
      601 gccgataaag cagccccagc caatgcagat ttaaatatga actctcttct tttcatcaat
      661 ttgcctggaa ataaataaat ttaaattatg tttaatataa aattattctt aattaacgcg
      721 aaggaacttt gcatttattg caacaataat agtgctcgca gacataagaa cagcacccaa
      781 cgcaggactt agtactattc caaaattata aagcactccc gcagcaagtg gaattgccac
      841 cacattataa cctgttgccc aaattaaatt ttgaatcatc ttactgtaag ttgcttttgc
      901 aaattttatt aatgaaacca catccttggg attactcttt acaagaatta tatctgcagt
      961 ctcaacagct acatccgttc ctgttcctat cgcaattccc acatcagatt gtgcaagtgc
     1021 gggtgcatca ttaactccgt cacctgtcat cgcaacaact aatccacggc tttgcacttc
     1081 ttttactttc tcagcttttt tatcaggcat aacttctgca aagtacactt caagctcaag
     1141 ttcatcggca acccattttg ctactcgctt attatctccc gtaagcatca tcggtctaat
     1201 tcccaattct ttaagctgct taattgtttc cttcgattct tctctgatta tatctgctaa
     1261 tgctattgcg ccgattagtt tattatcaat aagaacaaat acaacggtct taccctgagc
     1321 agatattttt tcaattttat ctttttcctg cgattcaatt ttattttcct cgagatatct
     1381 cggactcaca accttaacat ctttgccgtt tacttttcct tctgctcctt ttccgggtat
     1441 tgaattaaat tctttcaact catatttatc ctcagaagat ttaactatcc cctgagcgat
     1501 tggatgctca gattcagatt caagtgaagc agcatatttc aagatttctt tttcaccata
     1561 gtttttatcg aatgaaattg tctctgtaat tccgaattca ccttttgtta aagtcccggt
     1621 cttatcaaaa ataattgcct gaatattcct tgcctgttca aatgcgtttc tatttcttat
     1681 tagcaaacaa ctttttgcag aaagagct
//
LOCUS       DASWBY010000374         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_728966, whole genome shotgun sequence.
ACCESSION   DASWBY010000374 DASWBY010000000
VERSION     DASWBY010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728966"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="VF870_06845"
     CDS             complement(<1..1429)
                     /locus_tag="VF870_06845"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9251942.1"
                     /translation="MRLNGGTITGKVFDYDSKHSIEYANIVLLSPVDSSLINGTITDE
                     NGLFSLSGIKSGKYLVQVRFIGYKNENFNVEITEADKDIDLGEIFIRPNALELNDIVV
                     QGERSPVSYQIDKKVIDVDKIQTVMSGNAADVLRNIPSVTVDIEGNVSLRGSVNFTVL
                     IDGRPSILSAQDALQQIPASSIQNIEIITNPSAKYDPEGPAGIINILLKKNENLGLSG
                     VINANIGLNEKYGGDFLFQYRTSNLTYLLGMDYNKRKFPGTSISENTYISEGSTSYLN
                     SNGDSEWGRTGFGLRAGFEVNFTENDFINFTGRYGTREGGQNSLLNYSEWSTISPDVL
                     YYNNTTDRSRDGNYGGANFTYIHNFGGKGHEIKNEINFRYNDGDETTLTQSLNGETIL
                     EGKKTTEYGPSRDLEIKIDYTLPFLEVKRLEAGYEAELDNSDDNNALYEYDPLSDQYL
                     LQPQFSNSVNYKDNYHALYATYLDQF"
BASE COUNT          521 a          295 c          239 g          588 t
ORIGIN      
        1 caaactgatc aagataagtt gcatataaag catggtaatt gtctttataa tttactgaat
       61 tgctaaattg cggttgtaat agatattgat cgcttaaagg atcatattca tacaaagcat
      121 tattatcatc tgaattatcc aattcagctt cataaccagc ttcaagtctt ttaacttcta
      181 agaatggaag agtgtaatca atttttattt ctaaatctct ggaaggtcca tattcagttg
      241 ttttcttacc ttctaaaata gtttcaccat tcagcgattg agttaaagtc gtttcatcgc
      301 catcattata tctaaaattg atttcatttt taatttcatg tcctttgcct ccaaagttat
      361 gaatataagt aaagtttgct ccgccataat tcccatctct acttctatca gtagtattat
      421 tgtaatataa tacatcggga gaaatggttg accattcact ataattcagt aaggagtttt
      481 gtccaccttc ccttgtacca tatcggccag taaaatttat aaaatcattt tcagtaaagt
      541 taacttcaaa tccggctctt aatccaaatc cggttcttcc ccattctgaa tctccattgg
      601 aatttaaata tgaagttgag ccctcagaaa tataggtatt ctcagagatc gatgttccgg
      661 gaaattttct tttgttgtaa tccatcccaa gtaaataagt taaattgctc gtgcgatact
      721 gaaaaagaaa atctccacca tatttttcat ttaatccgat gtttgcattg atcacaccgc
      781 tcaagccaag attttcgttt ttcttaagca aaatatttat aatacctgca ggtccttctg
      841 gatcatattt tgcggatggg tttgtgatta tttcaatatt ctggattgaa cttgccggga
      901 tctgctgcaa ggcatcctgc gcacttaaaa tagatggtct gccatcaata agaacagtaa
      961 aattaacgct gccccgtaaa cttacattac cttcaatatc aacagttacg gatggaatgt
     1021 ttcttaatac atccgccgca ttacccgaca ttacagtttg gattttatct acatcgatta
     1081 cttttttatc tatctggtat gatactggtg atctttcacc ttgcacaaca atatcattaa
     1141 gttcaagagc atttggtctt ataaatattt caccaagatc aatatcttta tctgcttcag
     1201 taatttcaac attaaaattt tcatttttat aaccaataaa tcttacctgg accaaatatt
     1261 tacctgactt aattccggat aaactaaaaa gtccgttttc atcagtaatg gtaccgttaa
     1321 ttagtgatga atcaactgga gaaagtaaaa caatgtttgc atattctata gaatgctttg
     1381 aatcataatc aaacactttc ccggtgattg tgccaccgtt taatcgcatc ggatttgctt
     1441 gtggaaaaag ttgtatgttt ataattatta aggctataaa aaatattatt ttcataaaaa
     1501 taaattacta aggagaatct ttttaaaaaa gttatttaat tatcttgtga cgttgttcaa
     1561 ttgatacaaa gacaaattac tgaatattga atccggcatt tacaataagc agttttgctt
     1621 ttgaagcatc gctttcagat tca
//
LOCUS       DASWBY010000375         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_128482, whole genome shotgun sequence.
ACCESSION   DASWBY010000375 DASWBY010000000
VERSION     DASWBY010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128482"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VF870_06850"
     CDS             <1..407
                     /locus_tag="VF870_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9251943.1"
                     /translation="LHTSNETVLKVKEFASRIGKTPVEVYEYPGFITTRAIVPLINEA
                     MHILLEGIATAKDIDTALKLGYNFQYGPLEMADMMGLDEVLAWMETLWKTLGEPRYRP
                     NPLIRKLVRERRLGKKTGEGIFKYDEHGNKIN"
     gene            449..1648
                     /locus_tag="VF870_06855"
     CDS             449..1648
                     /locus_tag="VF870_06855"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX9251944.1"
                     /translation="MKVLVLNSGSSSIKYQFIDTEKKIALAKGLVDRIGMAGAVLSHQ
                     RYDGDNIKIAGEILDHQIAVEYVLGVMLSKNHGVIDDKKDIEAVGHRVVHGGETFSGS
                     VYVTDEVVKVLQDNIELAPLHNPPNIKGIQACQRILPDTPQCGVFDTAFHSHMPPKSY
                     LYGIPYELYKKYKIRRYGFHGTSHLYVSNRAAELLGKNISELKVITAHLGNGCSMAAV
                     KNGKSMDTTMGFTPLEGLLMGTRSGDLDPSLILYIMGKEGLTVGEANTLLNKHSGLIG
                     ISGESSDMREILSAVKDNQQRAKWAFEIFCYRIKKYVGAYAAAMGGLDALVFTGGIGE
                     NSKEIREEVCKELEFLGINLDDFKNQNADEIISTENSKVSVMRIPTNEELVIALDTAT
                     IVSEMQK"
     gene            1678..>2302
                     /locus_tag="VF870_06860"
     CDS             1678..>2302
                     /locus_tag="VF870_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9251945.1"
                     /translation="MDLGIKGKVALITASSTGIGKAVAEALAEEGCNIAICSRTKKVL
                     IETARDIKSKFNIEPFWGVCDLNQQKDIENFYNAVINQFGKVDILVNNCGGPIPGFFR
                     DLSEEDWDQAFKQVLLSVVRFSNLVIPKMIEQEWGRIINITSIAVKQPVHNLMLSNSL
                     RAGVTGFAKSLSNEFGSKNITINNVAPGYTLTHRLYELAVNRAKSSGK"
BASE COUNT          789 a          334 c          481 g          698 t
ORIGIN      
        1 gtcttcatac atcaaacgaa acagttttaa aagtcaaaga atttgcttca agaatcggca
       61 aaacacctgt tgaggtttat gaatatcctg ggtttattac aacacgcgcg atcgttcctc
      121 taattaatga agcaatgcat attcttcttg aaggaattgc tacggctaag gatattgata
      181 cagctttaaa gctcggttac aattttcaat atggtccgct tgaaatggca gatatgatgg
      241 gattggatga agtgttagcc tggatggaaa ctttgtggaa aacgctaggt gaaccaagat
      301 atagaccaaa tcctttgatt agaaaacttg tacgtgaaag aagacttggt aaaaaaacag
      361 gtgaggggat ttttaaatat gacgaacacg gaaataaaat aaattaattt ctttttcggg
      421 taagtagaat taaattggaa aaaatattat gaaagtattg gttcttaaca gcggtagttc
      481 ttcaataaaa tatcagttta tagacacaga aaaaaaaata gcattagcta aaggattagt
      541 agatagaatt ggaatggctg gtgctgtcct atcacatcaa agatacgatg gtgataacat
      601 aaaaatcgcc ggtgaaattt tagatcatca aattgctgtt gaatatgtac ttggagttat
      661 gttaagtaaa aaccatggtg taattgatga taagaaagat attgaagcag tcggccatcg
      721 ggttgttcac ggtggggaaa cattttccgg atcagtttat gttactgatg aagttgtaaa
      781 agtgctgcag gataatatag aacttgcacc attgcataac ccgccaaaca taaagggtat
      841 tcaagcctgc cagagaattt tacctgatac accacagtgc ggtgtttttg ataccgcttt
      901 tcattctcat atgcctccta aatcttattt gtacggtatt ccttacgaac tttataaaaa
      961 atataaaatt cgtcgttatg gatttcatgg cacatctcat ttatatgtat ctaatcgtgc
     1021 agcagaatta ttagggaaga acatttcaga gttaaaagtt ataactgctc atttgggaaa
     1081 tggttgtagt atggctgcag taaaaaatgg aaaatcaatg gataccacta tgggattcac
     1141 tccgctggaa ggactgttaa tgggtacgcg cagcggtgat cttgatccat ctttaatatt
     1201 atatattatg ggtaaagaag gattaacagt aggtgaagca aatacattac ttaacaaaca
     1261 tagcggtttg atcggtataa gcggcgagag cagcgatatg agagaaattt tatcagcggt
     1321 aaaagataat caacaaagag ccaaatgggc atttgagatt ttctgttata gaattaagaa
     1381 atatgtagga gcatacgccg ctgctatggg cggtctggat gctcttgtat ttactggtgg
     1441 aattggtgaa aattcaaaag agatacgtga agaggtttgt aaagaattgg aatttttagg
     1501 aattaattta gatgatttca aaaatcagaa tgctgatgaa attatttcta ctgaaaattc
     1561 taaagtttcc gttatgagaa ttccaaccaa tgaagaactg gttattgctc ttgatactgc
     1621 aacaattgtt tctgaaatgc aaaaataatt tttattaaca tttccatagg ctgaattatg
     1681 gatctcggaa taaaaggtaa agtagcttta attacagctt ccagcaccgg aattggaaaa
     1741 gcagttgccg aagcgctggc agaagaaggt tgtaatattg caatttgttc ccgcactaaa
     1801 aaagttctta ttgaaactgc tcgtgatatt aagagtaaat ttaatattga gcctttctgg
     1861 ggagtatgcg atttaaatca gcaaaaggat attgaaaatt tttataatgc agttataaat
     1921 caatttggta aagttgatat tttagtaaac aattgcggcg gaccaatacc aggttttttt
     1981 cgggatcttt cagaagaaga ctgggatcag gctttcaaac aagttttact aagcgtggtt
     2041 agattttcca atcttgttat tcctaaaatg atagagcagg aatggggaag aataattaac
     2101 ataacttcca ttgctgttaa gcagccggtt cataatttaa tgctttcaaa ctcattacgt
     2161 gcgggtgtaa caggattcgc aaagtcttta agcaacgagt ttggaagtaa aaacattact
     2221 ataaacaatg ttgcccctgg ctatacatta acccacagat tatatgagct tgcagtaaat
     2281 cgagcaaaat catcaggcaa at
//
LOCUS       DASWBY010000376         5248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_315612, whole genome shotgun sequence.
ACCESSION   DASWBY010000376 DASWBY010000000
VERSION     DASWBY010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315612"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VF870_06865"
     CDS             <1..1354
                     /locus_tag="VF870_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9251946.1"
                     /translation="VLDLSKMESGKMKLEFEKTDIVLLLKNRVASFDSLAEQNKILMK
                     FESKIESLLLNIDKEKIEEVIDNLLSNAFKFTPPNGNITMLLDQERSEFTANAVITIS
                     DNGIGIPEEKLVNIFDRFYQADSSSTKQYEGTGLGLAIVKEIVELHGGTITVNSKINT
                     GTSFSIYLPVEETEIETAFSEIEEIKALEKNKNEENKSLILIVEDNHDVRSFIKENLD
                     EKYRIEEATNGEEGISKAIEKIPDLIITDVMMPKVNGFELCNKLKNDHRTSHIPIVIL
                     TAKADEEDKIDGLQIGADEFLAKPFSSRELEIRVGNLVHIRQLLREKYKEISVIKSED
                     IKANPIDKDFLDKVFGLIKNHIEDQQFSVQKLADNMAMSVSQLNRKLNALINQSAGKL
                     IRSTKLDYAAKLLEKNAGNITEIGYRIGFSDLPSFTNSFKEKFGVSPSEYLKSRKENI
                     "
     gene            1469..3094
                     /locus_tag="VF870_06870"
     CDS             1469..3094
                     /locus_tag="VF870_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251947.1"
                     /translation="MKRNFTNLLTLAILTFTSLQLSAQTTYEIIDIGSGGLSAVSKNG
                     QYACGMSYPNPGYIWNEVTGRVDLNPSTEFSEAYDVSNDGIVVGRFYDPTLPAPGGNP
                     TLRAGYWENGTWNALAGLVGLVPLDEISFTHAYGISADGSRIVGMQWHPDYSVEAVYW
                     DDASLATGLGQTYGENSRANCVSSIGLIMAGWNAGPDGIPDRTAFSWDPAPHYLGGFD
                     QSYPVGESRAINSDGTIIVGGAIWPFIWSTSTGMKQIVADSSDYEQGAAQGISDNGTI
                     VGWVNPVGGGFNYKGFIKKSNWPDIVYLSTYIADSLGITGYTDWDFAWGQSISADGKI
                     IGMTAYTPLGEPRALLLRINEPVPVELISFTASANNSTVNLNWTTATELNNSGFEIQR
                     KNENSDWISIGFIKGFGTTTTQNKYSYTDQNPFVGNNIYRLKQLDFNGTFEYSKEVEV
                     GIVLSEYVLQQNYPNPFNPTTTINFNIPKEDFVNVTIFNSLGEKVATLLDGVMSAGSH
                     ALTFDANGFASGLYILKMSSGGYSNTIKMNLMK"
     gene            3122..4741
                     /locus_tag="VF870_06875"
     CDS             3122..4741
                     /locus_tag="VF870_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9251948.1"
                     /translation="MKKNLTSYLFTIVFIFLSSHLSAQTTAQLINLGYGGAAKISNNG
                     LYVCGNNYPSPGFIWSESSGRVNLGSGYTEAFGVSDNGVVAGTYLDSTLLDPYGKPTM
                     RAGYFEHNQWNALPGYPSYPPLDSMTYTVGYGISGDGTMIVGMHWVPGYKAEAVYWDS
                     QRDIHLLGRTGGGSSRANDVAVTDTGFVIVGWDGLVNGPGRRAFYWDPTPHFMGGYEP
                     SYPDGQCEGLNSDGSKIVGGSVGAPFVWTQAAGMKWITTDYLNYASYAKDISDNDIVV
                     GYVSIGVGNYQAFIKRPEWNDIMLLKNYMVDSLGITEAADWLYSFANSISADGLTIVG
                     TAYPLAGGPIAYVLKFENPVPVELTSFSASYNNNKVTLGWITSSELNNQGFEVERKTQ
                     SSEWNKIGFVNGNGTTTETNSYQFIDNSISSNKYYYRLKQVDLDGTFEYSNIVEVDIN
                     TVTEFSLNQNYPNPFNPSTKISFTLPQTANVKLSVFNLLGEKVAELVNETKGPGFYNV
                     DFNGSNLTSGMYLYRLEAGEFVSTRKMTLIK"
     gene            4781..>5248
                     /locus_tag="VF870_06880"
     CDS             4781..>5248
                     /locus_tag="VF870_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251949.1"
                     /translation="MCKKLTVFFTVVIMLLAGYSIQAQTTAYVFNSALYSYGTVDLST
                     GAFTSSNFFSPGYNMQAVTGDNYGTNEQYAIMANMAFNTFYLWDINFNTLSGDSIGIV
                     GPLAAGQTAVKAMAYNSLTDTWYVLSSNDFGTAAYLYTLNITNASLTQVAQLTG"
BASE COUNT         1787 a          818 c         1039 g         1604 t
ORIGIN      
        1 ggtattggat ttatcaaaaa tggaatcggg aaaaatgaaa ctggaatttg aaaagaccga
       61 tattgtttta ttattaaaaa accgtgttgc ttcatttgat tctttagccg aacaaaataa
      121 aatattaatg aagtttgaaa gtaaaataga atcactctta ctaaatattg ataaagaaaa
      181 aattgaagaa gtaattgata atcttctctc aaatgctttc aagtttactc ctcctaatgg
      241 aaatatcact atgttacttg atcaggaaag atctgagttt actgctaatg cagtaattac
      301 aatcagcgat aatggaattg gaattcctga agaaaaactt gtaaatatat ttgatagatt
      361 ttatcaagca gacagctctt cgacaaaaca atatgagggc actggacttg gattagccat
      421 tgtaaaagaa attgtagaat tgcatggcgg cacaataacg gttaacagca aaataaatac
      481 tggtacaagt tttagtatat atcttccagt agaagaaaca gaaatagaaa cagcattttc
      541 tgaaattgaa gaaataaagg cattagaaaa aaataagaac gaagaaaata aatcactgat
      601 attgattgtt gaagacaatc acgatgtaag aagttttata aaagaaaatt tagacgaaaa
      661 gtacagaatt gaagaagcta caaatggcga agagggtatt agtaaggcaa ttgaaaagat
      721 tcctgattta attattacag atgtaatgat gcctaaggtt aatggttttg aactttgtaa
      781 taaattaaaa aatgatcaca gaacaagcca tattcccatc gttattttaa ccgcaaaagc
      841 ggatgaagaa gataaaatag acggacttca aataggcgcg gatgaatttc ttgcaaaacc
      901 atttagttcc cgagaacttg aaatccgtgt tggaaacctt gtacacatta gacagctttt
      961 aagagaaaag tataaagaaa tatctgtcat taaatccgaa gatataaaag caaatcctat
     1021 tgataaggat tttttagata aagtttttgg cttaattaag aaccatattg aagaccagca
     1081 atttagtgta caaaaattag ctgataatat ggcaatgagt gtttctcagc tgaacagaaa
     1141 attaaatgcg cttattaatc aatcagcggg taaactaatt cgttcaacaa agcttgatta
     1201 tgccgcaaag cttcttgaaa aaaatgcagg caatattacc gaaattggtt atagaatagg
     1261 tttctcagat ttgccaagtt ttaccaacag ttttaaggaa aaatttggag tttcaccctc
     1321 tgaatatctt aagtctagaa aagaaaacat ttaattttta acaaaatcaa agcctgctca
     1381 aaaatcccaa ctatttgccc gatcagacta ataaaatctt tagatattga gttacgtttg
     1441 tctatacaaa tttaaaaagg tgaacccatt gaaaagaaat tttactaatc ttttaacatt
     1501 agcaatttta acgtttacat ctcttcaatt atctgcacaa acaacctatg aaattattga
     1561 tattgggtca ggtggcttaa gtgcagtttc aaaaaatgga caatatgcct gcggaatgag
     1621 ctatcctaat cccggatata tctggaacga agttacaggt agagttgatc tcaacccttc
     1681 gactgaattt tctgaagcct atgatgtttc caatgatggt attgtggtgg gcagatttta
     1741 cgatccaaca ttacctgcac cgggtgggaa cccaacttta agagctggtt attgggaaaa
     1801 cggaacatgg aatgccttag cagggcttgt gggtttagtt cctttggatg aaataagttt
     1861 tacacatgct tacggaattt cagcagatgg ctcaagaatc gtaggtatgc aatggcaccc
     1921 ggattattcg gttgaggccg tttattggga tgatgccagc ctggcaacgg gtttggggca
     1981 gacctacggt gaaaatagtc gtgctaattg tgtctcatca attggattaa taatggccgg
     2041 ctggaatgct ggaccagatg gaataccgga tagaacagcc ttttcgtggg atcctgctcc
     2101 tcactattta ggtggttttg atcagtcata cccggtgggc gaatcaagag caattaattc
     2161 agacggtaca atcatagttg gcggagcgat atggccattt atatggtcta catcaactgg
     2221 aatgaaacaa atcgttgctg actcttcaga ttatgaacag ggggctgctc aaggaatttc
     2281 ggataatgga acaatcgttg gatgggttaa tcctgtgggt ggtggattta actacaaggg
     2341 gtttattaag aaatctaact ggcctgatat tgtctatctt tcgacatata tcgcagacag
     2401 ccttggtatt actggttaca ctgattggga ttttgcttgg ggtcagtcaa tatctgcaga
     2461 tggtaaaatc attgggatga cggcatatac accactgggt gaaccaagag cacttctgtt
     2521 aagaattaac gaaccagttc ctgttgaatt aatttcattt acagcttctg ctaataattc
     2581 aactgtaaat cttaattgga ctactgctac agagctaaac aattccggtt ttgaaattca
     2641 aagaaaaaat gaaaattctg attggatttc gattggattt attaaagggt ttggaacaac
     2701 cacaactcaa aataaatata gttacacaga tcaaaatcca tttgttggta ataatatcta
     2761 tagattaaaa cagttagact ttaatggaac ttttgagtat tcaaaagaag ttgaagtagg
     2821 aattgttcta tctgaatatg ttttacagca aaactatcca aacccattta acccgacaac
     2881 tacaataaat tttaatatac ccaaagaaga ttttgtaaat gtaacaattt ttaattcgct
     2941 tggagaaaaa gttgcaacat tattggatgg tgttatgagt gctggttcac atgcattaac
     3001 gtttgatgca aatggttttg cgagcggctt gtatattcta aaaatgtcat ctggcggtta
     3061 ttcaaatacc attaaaatga acttgatgaa ataattctgc aaataaaatg aaagaaaagt
     3121 catgaaaaaa aatcttacta gttatttatt tactatcgta ttcatttttt tatcatctca
     3181 tttatcagca caaacaacag cccaattaat taatctggga tatggtggtg ctgcaaaaat
     3241 atcaaataat ggtttatatg tctgcgggaa caattatccc tcccccggtt ttatatggtc
     3301 ggaatcatca ggaagagtta atcttggttc tggttataca gaggcttttg gagtttctga
     3361 taatggagtg gtagccggaa catatctgga ttcaaccctt ttagatccat acggaaaacc
     3421 aacaatgaga gccggttatt ttgaacataa ccagtggaat gctttacctg gttatccttc
     3481 ttatccgcct cttgattcaa tgacttatac agtgggctac gggattagtg gtgatggaac
     3541 aatgatagtt ggaatgcatt gggttccggg atataaagca gaagcagttt actgggatag
     3601 tcaaagagat atccaccttc tgggcagaac cggcggcggc agcagccgtg caaatgatgt
     3661 tgcagtaacc gatacgggtt ttgttattgt tggttgggac ggtttggtaa atggtcctgg
     3721 aagacgtgct ttttattggg atccaactcc tcactttatg ggtggatacg aaccatctta
     3781 tcccgatgga caatgtgagg gtttaaattc tgatggaagt aaaatagttg gaggttcggt
     3841 gggagcgcca tttgtttgga cacaggcagc tggaatgaaa tggattacta ctgattatct
     3901 taactatgcc agttatgcaa aggatatttc cgataatgac atagtagttg gttatgttag
     3961 tattggtgta ggtaattatc aagcttttat aaaaagacct gaatggaacg atataatgct
     4021 gcttaaaaac tatatggttg atagcctggg cattacagag gctgcagatt ggctttattc
     4081 ctttgcaaac tcaatttcag cagatggatt aactatagtt ggtacagctt atcctttagc
     4141 cggaggtcca attgcatatg tacttaagtt tgaaaatccc gtgcctgttg agctaacctc
     4201 attttctgct tcctataata acaataaagt aactcttggc tggataacaa gcagtgagtt
     4261 aaataatcag ggatttgaag ttgagcgtaa aactcaaagc tcagaatgga ataaaatcgg
     4321 atttgtaaat ggaaatggca caacgactga aacaaattct tatcagttta tagataattc
     4381 aatctcatca aacaaatatt actatagact taaacaagtt gatttagacg gaacatttga
     4441 atattcaaat attgttgagg ttgatataaa cacagttaca gaattttcat taaaccagaa
     4501 ttatccgaat ccatttaatc catctacaaa aatttctttc actctacctc agactgcgaa
     4561 tgtgaaatta tctgtattca atttacttgg tgaaaaagtt gcggaacttg ttaatgaaac
     4621 taaaggcccg ggtttttata atgtagattt taatggttcc aatttaacaa gcggaatgta
     4681 tttatatcgt ttagaagccg gtgagtttgt ttcaacaaga aagatgacat taattaaata
     4741 atctaactaa ctctaatcaa ataaataata ggagaatcct atgtgtaaaa aattgaccgt
     4801 tttcttcaca gttgttataa tgcttcttgc tggttatagc attcaggcac aaacaacagc
     4861 ttatgtattc aacagtgcgc tatatagtta tggcacagta gatttaagta caggagcatt
     4921 tacctcatcg aatttctttt ctcctggtta caatatgcag gcagtaacag gagataacta
     4981 tggaacaaat gagcaatatg caataatggc aaatatggca ttcaacacat tttacttatg
     5041 ggacataaac tttaatacat taagtggtga tagtattgga attgtaggtc ctctcgcagc
     5101 aggacaaaca gcagtaaaag caatggcata taattcacta acagatacgt ggtatgtttt
     5161 aagttcaaat gattttggaa cagcagcata tttgtacaca ctaaacatta caaatgcatc
     5221 cttaacacag gttgcacagt taacaggt
//
LOCUS       DASWBY010000377         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_50724, whole genome shotgun sequence.
ACCESSION   DASWBY010000377 DASWBY010000000
VERSION     DASWBY010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50724"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..>2135)
                     /locus_tag="VF870_06885"
     CDS             complement(259..>2135)
                     /locus_tag="VF870_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9251950.1"
                     /translation="QILSALGLDAMTLGNHEFDLGPEVLLGSLQYAFPVANDAFPLLS
                     ANIDFSDPSIIGMQDYVKPYVIKQYGSTKVGIFGLTTPATNLTSMPSPAVVSEDIVNI
                     AVEMVNTLRTVENCDIVILLSHLGVALDQLIVSYVPGINVIVGGHDHYMYEQPITLTD
                     PLGGTTWIVQARSNYMYAGEMKLVVDNTNVQLLNYQLIPIDENIPQEPTVQAIVDGMI
                     TDIESTYGIPFFTQPFGNATAFFEEEAKDLLALGPHDTPMGNLVSDAFRSLTSTDIAL
                     QAGGSIALPLWEGTFTAGDLFRVNGYGFNTTNTLGFQLATYDLTGMDIMKGLTFGLSE
                     IEMSDEYFLQVSGMEYTYDGTKSAEERLVSVKINGNPIDPMATYSVTTNEMLLGFMSY
                     LGIVPANLNILTGVTEFEALLTYTNNQQNILTPSTNGRVSNVTSPVPVELTSFSANIV
                     DNFVNLRWVTATEINNHGFEIERSLNKSIWTIIGFKEGKGTTTETQQYSYSDNISEIS
                     SSKLYYRLKQTDFNGNFEYSDIVEIEIAPFTYLLDQNYPNPFNPSTTINYSIPEAGNV
                     EMKVYDILGNEVATLVNEAKAPGNYSAVFDASSLASGIYFYKLSANNFLQTKKMILMK
                     "
BASE COUNT          682 a          392 c          353 g          708 t
ORIGIN      
        1 tgctatacaa ttagttgaat ctaaaaaata acaatcgtaa aattcgtcag cagttgggta
       61 aggatttacc caactccatt ttgattcctg ggcaaaaaag ttagtactta gaaccagaaa
      121 tgtaagcgat agctttagta atggtttcat tatatactcc atttctactt taatcttaaa
      181 tatttatcat tttaatttga gatttacaat ttaaacagcc cccaatttta ttttagctgt
      241 ctgaaaataa acagatagtt atttcattaa aatcatcttc ttagtttgaa gaaagttatt
      301 cgcacttaac ttgtagaaat aaattccact tgccaacgat gaggcatcaa aaactgcaga
      361 ataattacct ggtgcttttg cttcatttac cagagttgca acttcattac caagtatatc
      421 ataaaccttc atctcaacat ttcccgcttc cggaattgag tagttaattg ttgttgacgg
      481 gttgaatggg tttggataat tctggtctaa taaatatgtg aagggtgcaa tttcaatttc
      541 gacaatatct gagtactcaa aatttccatt gaaatcagtt tgttttaatc tgtagtaaag
      601 ctttgatgat gatatttctg aaatattatc tgaataagaa tattgctgtg tttctgttgt
      661 tgttccttta ccttctttaa aaccgataat tgtccaaatt gatttattca aacttctttc
      721 aatttcaaat ccgtggttgt ttatttcggt tgcagtaacc catctaagat taacaaaatt
      781 atcaacaatg tttgcagaaa atgaagttag ctcaacagga actggactgg ttacatttga
      841 aactcttcca ttggtagaag gcgttaaaat gttctgttga ttatttgtat aagttaaaag
      901 agcttcaaat tctgtaaccc cagtaagaat attcaagttt gcaggtacta tcccaagata
      961 agacataaaa cctaataaca tttcattagt agtaactgaa taagttgcca ttggatcaat
     1021 cggatttcca ttaattttaa ctgatactaa tctttcctct gcggatttgg ttccatcata
     1081 tgtatattcc attccggaga cttgcaggaa atattcatcg ctcatttcta tctcagaaag
     1141 tccgaatgta agtcctttca taatatccat tcccgtcaaa tcgtatgtag caagttgaaa
     1201 accaagagtg tttgttgtat tgaatccata cccattaact ctgaagagat ctccagctgt
     1261 aaatgtacct tcccataaag gaagagctat tgatcctccc gcctgcagcg caatatccgt
     1321 tgaagttaat gatctgaaag catcagaaac taaattaccc attggagtat catgtggccc
     1381 aagtgctaaa agatctttag cttcctcttc aaaaaacgca gtagcattac caaatggttg
     1441 agtgaaaaaa ggaattccat aagttgattc tatatcggta atcattccat caacaattgc
     1501 ctggactgtg ggctcttgcg gaatgttttc atcaatagga attaattgat aatttaataa
     1561 ctgaacattt gtgttatcaa caactaactt catctcaccg gcatacatat aatttgatct
     1621 tgcctgaact atccatgtgg ttccacctaa tggatctgtc aatgttatcg gttgttcgta
     1681 catataatga tcatgcccac cgacaattac atttattcct ggcacataac tgacaattag
     1741 ctgatcaagt gcaaccccga gatgtgataa taaaattaca atgtcgcagt tttcaactgt
     1801 tcgcagagta ttgaccattt ctaccgcaat gtttacaatg tcttcgctta caactgcagg
     1861 tgaaggcatt gaagttaaat ttgttgcggg agtagttagt ccgaatattc caaccttggt
     1921 gcttccatat tgtttgatta catatggctt aacataatcc tgcattccaa taattgaggg
     1981 atctgaaaaa tctatatttg ctgaaagcag aggaaatgca tcgttagcaa ccggaaaagc
     2041 atattgcaaa gaaccgagta gcacctcagg acccaaatca aactcatgat taccaagtgt
     2101 cattgcatca agtcctaatg cacttagaat ttgaa
//
LOCUS       DASWBY010000378         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_578710, whole genome shotgun sequence.
ACCESSION   DASWBY010000378 DASWBY010000000
VERSION     DASWBY010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578710"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VF870_06890"
     CDS             complement(<1..600)
                     /locus_tag="VF870_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9251951.1"
                     /translation="MSEVLDKKIDFSLKYKVKDISLAEWGRKEIELAEVEMPGLMALR
                     EEFKGKKPLKGARIAGCLHMTIQTAVLIETLVELGAEVKWSSCNIFSTQDHAAAAVAA
                     AGVPVFAWKGMNEEEFNWCIEQTLFFGDESRPLNMILDDGGDLTNMVLDVYPELAAGI
                     KGLSEETTTGVHRLYERMEKGTLPIPAINVNDSVTKSKFD"
     gene            complement(695..1843)
                     /gene="metK"
                     /locus_tag="VF870_06895"
     CDS             complement(695..1843)
                     /gene="metK"
                     /locus_tag="VF870_06895"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9251952.1"
                     /translation="MSFFFTSESVSEGHPDKVSDAISDGILDAIYEHDPHARVACETF
                     VTTGLVLVGGEITTKTYIDVPAIVRKTVENIGYTKADYKFDSESCSVLNAIHSQSPDI
                     AMGVDTGGAGDQGIMFGYACDQTPELMPMPIVFAHKLVKKLADIRKKNAKLMPYLRPD
                     SKSQVTIEYDDNGKPLRVDAVVISTQHDGDAKQSKIKSDVIQHVIKKVIPAKYLDKNT
                     KYFVNPTGRFEIGGPHGDSGLTGRKIIVDTYGGWAPHGGGAFSGKDPSKVDRSATYAA
                     RHIAKNVVAAKLAKECLVQVSYAIGVAQPISVFVDTKGTGVIPDSEISKMIQKEVDLT
                     PKGIISRLKLRRPIYQKTSAYGHFGRNDKDFTWEHLDLVPVFKKYAKK"
     gene            1989..2948
                     /locus_tag="VF870_06900"
     CDS             1989..2948
                     /locus_tag="VF870_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9251953.1"
                     /translation="MKNRITQLLNIKYPIIQAGMVWVSGSKLASAVSNCGGLGLIGAG
                     SMKPELLKEHLQKCKTATNKPFGVNIPLLRKDANDLLQTCLGEGVKIFFTSAGNPKTF
                     TPLLKENGCTVVHVVANLKYGLKAQEAGCDAVVGEGVEAGGHNGADQLTTFCLIPQLV
                     DKLNIPVISAGGIADGRGILAAFSLGAEGVQIGTRFAASVESSAHDNYKQEIVKSGDQ
                     DTTLAFKKIGLVRMIKNDFAFRAMKAENEGWNEARLKELLGVKRERTGIFDGDLIEGE
                     LEAGQVVGLINDIPTVKDLFDRLLKEYKIAKANLRQWNADDRD"
BASE COUNT          932 a          575 c          534 g         1028 t
ORIGIN      
        1 atcaaactta gattttgtaa ccgaatcatt tacgttgatt gccggaattg gaagtgttcc
       61 cttttccatt ctttcatata aacgatgtac accggttgtt gtctcttcgg ataatccttt
      121 aatgcctgcg gctaattcag ggtaaacatc aagaaccata tttgtaagat caccgccatc
      181 atcaagaatc atatttaatg gtcttgattc atcaccaaaa aataatgttt gttcgatgca
      241 ccagttaaat tcttcttcgt tcattccctt ccatgcaaat acaggaactc cagcagctgc
      301 aactgcagca gcggcatgat cctgagtaga aaatatatta cacgatgacc atttaacttc
      361 tgcgccaagt tctactaatg tttcaattaa cacagcagtt tgaattgtca tatgaaggca
      421 tccagcgata cgcgcacctt taagcggctt ctttccttta aactcttctc tcaatgccat
      481 caaaccgggc atttccactt cagctaattc aatttctttt cttccccatt cagccagaga
      541 tatatctttt actttatact ttaacgaaaa atcaattttt ttatctaata cttcactcat
      601 tttttaacct ttactttttt taataataat ttcttgtgat tattcattat taacagaaca
      661 acaaacaatc tataaaaagt tcacattctt aagattattt ttttgcatat ttcttaaaaa
      721 ccggaacaag atcaagatgt tcccacgtaa agtctttatc atttctgcca aagtgcccgt
      781 atgcagaagt cttttgataa ataggtcttc ttagttttaa tcttgagata atccctttag
      841 gtgtcagatc aacttctttc tgaatcattt tagaaatttc agaatcagga attacgcctg
      901 ttccttttgt atcaacaaaa acagaaattg gctgtgctac tccaattgca taagaaacct
      961 gaaccagaca ttctttagca agttttgcag ctacaacgtt ctttgcaata tgtcttgcgg
     1021 cataagtagc gcttctatcc accttgcttg gatctttacc tgaaaatgca ccgccgccat
     1081 gaggtgccca tccgccgtaa gtatcaacaa taatttttct tcctgtcaat ccgctgtctc
     1141 cgtgcggtcc accgatttca aatcttccgg ttggatttac aaaatatttt gtattcttat
     1201 caagatattt tgcagggata acttttttaa ttacgtgttg gattacatcc gatttaatct
     1261 tgctctgttt tgcatctcca tcgtgttgag tagaaattac aacagcatca actcttaatg
     1321 gttttccatt atcatcatat tcgattgtaa cttgtgattt tgaatcagga cgaaggtaag
     1381 gcattaattt tgcatttttc tttctgatat cagccagttt ttttacaagc ttatgtgcaa
     1441 acactattgg cattggcata agttcaggtg tttgatcaca agcatatcca aacataattc
     1501 cctgatcacc tgcaccgcct gtatcaactc ccattgcaat atccggtgat tgagaatgaa
     1561 ttgcatttaa tacagaacaa gattcagaat caaatttata atcagcttta gtgtacccaa
     1621 tattttcaac tgtctttcta actattgcgg gaacatcgat atatgtcttt gtagtaattt
     1681 caccaccgac taaaactaaa cctgttgtaa caaaagtttc gcaagctact cttgcgtgtg
     1741 gatcgtgctc ataaattgca tcaagtatac catcggatat cgcatcactt actttatccg
     1801 gatgaccttc agaaactgat tctgatgtga agaaaaatga catgttttac tattcctttg
     1861 aatgattttt aatatgctaa tatggttttt tgagtgccaa atttaactaa tcccttcatg
     1921 attacaaaat aagtgtgtta tattttggtt acatttgata tgtatttaat cacattattt
     1981 taatgacaat gaagaataga ataactcaat tactaaatat aaaatatcca atcattcaag
     2041 cagggatggt ttgggtctcc ggatcgaagt tagcttctgc cgtttctaat tgcggaggac
     2101 tgggcttgat tggtgccggt tcgatgaagc cagaattgtt gaaagaacat ttacaaaaat
     2161 gtaaaactgc aactaataaa ccatttggag taaatattcc tttattacgg aaagatgcaa
     2221 atgatctttt acaaacttgt cttggtgaag gcgttaagat tttctttaca tcggctggaa
     2281 atcccaaaac atttactcca ttattaaaag aaaatggatg caccgttgta cacgttgttg
     2341 caaatctaaa atatggattg aaagcccagg aagctggctg cgatgctgtt gttggtgaag
     2401 gtgttgaagc aggcggacat aacggtgctg atcagttaac tacgttttgt ttaattccgc
     2461 aattggttga taaactgaac attccagtta tatctgcggg aggaatcgct gatggaagag
     2521 gaattcttgc tgccttttct cttggtgccg aaggcgtaca aataggaact cgttttgcag
     2581 catcagttga atcttccgct cacgataatt ataaacagga aattgttaaa tcaggtgacc
     2641 aggacacaac gcttgctttt aaaaaaattg gtttagtcag aatgatcaaa aacgattttg
     2701 cattccgtgc aatgaaggca gaaaatgaag gctggaatga agcccgatta aaagaattac
     2761 tcggagttaa acgtgaacgt acaggtatat ttgatggcga tttaattgaa ggcgaacttg
     2821 aagccgggca agtagttggt ttgataaatg acattccgac tgttaaagat ttgtttgata
     2881 gattgctgaa agaatacaaa attgctaaag ctaatttaag acaatggaac gcggatgaca
     2941 gggattgaat aggtttccgc tgattgtatc ctagaatatt tgtctcatat aatttctgtt
     3001 atttaattag ctaaatatat ttcaaccact tcatcaacgt ctattgctgt tggaaagaaa
     3061 gaatgaatc
//
LOCUS       DASWBY010000379         5362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_614754, whole genome shotgun sequence.
ACCESSION   DASWBY010000379 DASWBY010000000
VERSION     DASWBY010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614754"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VF870_06905"
     CDS             complement(<1..650)
                     /locus_tag="VF870_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9251954.1"
                     /translation="MHLKNLLKVAIRSILKARMRSLLTALGIIIGVAAVVVMVAIGDG
                     AQIQVEQQISALGSNLIVIFPGSSTTGGINMGAGSINRFTMEDVEKIREEATLIKAVS
                     PIVRSGGQVIGGVGNWSTQVQGVATNFLEIRDWPLASGDFFTDKDMVSRSKVAVLGQT
                     VVKQLFPNDDPIGQQIRIRNVPFRVIGVLTGKGQSSFGTDMDDIIMAPATTVLDRLT"
     gene            complement(659..1417)
                     /locus_tag="VF870_06910"
     CDS             complement(659..1417)
                     /locus_tag="VF870_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9251955.1"
                     /translation="MPKEKNIMKIVGLTKTYVIGEVEVQALKSVDLSIDEGEFVAIMG
                     SSGSGKSTLMNLIGCLDKPTSGDYFLDGVNTSTLSKNDYAKIRNQKIGFVFQGFNLLA
                     RTTALENVELPLMYDRDQRIKNPAKKAIEVLDRVGLGDRIHHEPNQLSGGQQQRVAIA
                     RALVNEPSLILADEPTGNLDSKMSVEVFSLFQQLNDEGITIVLVTHERDFAKFAKRIV
                     ELKDGVVIKDYVINDRLIGTEELKKINENVHLEN"
     gene            complement(1423..2709)
                     /locus_tag="VF870_06915"
     CDS             complement(1423..2709)
                     /locus_tag="VF870_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9251956.1"
                     /translation="MKKKILIISSIIVIALVAIYFLFFNSSEAGTNKFTFATITRGDL
                     NTTITSTGTLQAVTTVDVGTQVSGKIDRLLVDFNNNVRKGQLLAVLDTTNLALQVNNA
                     LTGLQKAQASYDVAKATYERDQTLFDKKFMSELDFITSKSNYETAIANLNSAKSSLAQ
                     AKTNLSYAYIFSPIKGKIINRNVEEGQTVAASLSAPTLFTIAEDLSKMQILTNVDESD
                     IGQIKEGQDAQFTVQTYPDKKFTGKVFQIRLGSSVVSNVVNYIVVVNAENDDNLLLPG
                     MTATVDFYVEKRTNVLMIPNSALRFQPTDQMKADFTAKMQKEIKNMSDSLKQRFQSFG
                     GNSGMQNRGNGFGSSGNKSRGSFWYVDENGNPAMGFAQLGLSDGKNTEILRSRNLKEN
                     MQVITGFEIVADNKPTQNTNILNQNQSMPRSTRRGF"
     gene            complement(2759..3106)
                     /locus_tag="VF870_06920"
     CDS             complement(2759..3106)
                     /locus_tag="VF870_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251957.1"
                     /translation="MKKIILMSSLFLFMVTFASFAQGMRTPQERLKVLNEKLSLTKEQ
                     SVKVESILLENDKQIQKLRSSDNPDRNEFRKIMENSNQEILKVLNEKQKVEYNKMLEE
                     RKKQYQQNKNKEN"
     gene            complement(3455..4693)
                     /locus_tag="VF870_06925"
     CDS             complement(3455..4693)
                     /locus_tag="VF870_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX9251958.1"
                     /translation="MNLIEWTDQLRFLVAIALGFLVGLERESRGGSLSSKLTAGVRTY
                     TLISLFGFGCAWLFRLNIEFIIPVGIVVIGAMILASYLAKQKSGAVGWTSEIAALLIF
                     VVGVLSLLADIWIPMALGIFTTLLLSEKAELEKYIEKLDKSEFLAVVKFLIVTIIILP
                     ALPDQNYTRFNLNPYKIWQIVILVSSIGFVGYFLMKKFGSKVGIWLSGLLGGIASSTA
                     VSIAMGNIAKKNELIAKQAFQATILASSVMYIRILIIIYLLNVGIAYAIWWKLCILFF
                     VGLILAATIRDKNPEISTEPISTIQNPFEIRPAVIFAFLFVLLSIVTLWVKQFFGGGG
                     VLVLSGIVGVTDIDPFILSLINKQQPVGTIIESAIIISMTSNTIIKGIYFGYLAKGLR
                     KQVALYFFIWVVMHVIILFL"
     gene            4881..>5362
                     /locus_tag="VF870_06930"
     CDS             4881..>5362
                     /locus_tag="VF870_06930"
                     /inference="COORDINATES: protein motif:HMM:NF037701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251959.1"
                     /translation="MSEVFQAVIIKGIFIFLIFVMTIVVSSSAFFHPFSHIEVDNPNI
                     EYIGRFDFSKEGVVRFDWSGVQIKTKFSGPYCGIKLKDGKNDYNIFIDGKLYRILKTN
                     SDTLYTLADNLSDNVHTLLIAKRTEARNGLVSFGGLVLNSGKGLLSVSKESKRKIEFI
                     G"
BASE COUNT         1722 a          998 c          803 g         1839 t
ORIGIN      
        1 gttagtctgt caagcactgt agttgcagga gccataatta tatcatccat atcagtacca
       61 aaagaacttt gacctttacc tgtaagaact ccaattaccc taaaaggaac gttgcgtatt
      121 ctgatttgtt gaccaatagg atcatcattt gggaaaagct gcttaactac agtttgccca
      181 agaacagcaa cttttgatct ggaaaccata tctttatcag taaaaaaatc acctgatgct
      241 aaaggccagt ctctaatttc aagaaaattt gtagcaacac cttgcacctg cgtactccag
      301 tttccaacac cgccaataac ctgtccgcct gatcttacaa taggggagac agcttttatt
      361 aaagtggcct cttcccgaat cttttctaca tcctccatag taaatctatt tatactgcca
      421 gcacccatgt ttatacctcc agtggtacta gatcctggaa agattacaat taagtttgaa
      481 ccaagagcag agatttgctg ctctacctga atctgtgcac catcaccgat agctaccatt
      541 acaacaacag cagcaacacc gattataatt ccaagtgcag tgagcaggct gcgcattctt
      601 gcttttaaaa tacttctgat cgcaactttt aaaagattct ttagatgcat cttaacctct
      661 aattctcaag gtgaacattt tcattaattt ttttcaactc ctcagttcca attaatcgat
      721 catttattac ataatcttta atcacaacac catcttttaa ttcaacaatc cttttggcaa
      781 acttggcaaa atctctttcg tgtgtaacca atactattgt gatgccctca tcattcagtt
      841 gctggaataa tgaaaaaact tcaaccgaca ttttactatc cagatttccg gttggttcat
      901 ctgcaagaat taatgaaggt tcattcacca aagctcttgc aattgcaact cgttgctgct
      961 gtccaccgga aagttgattc ggttcatggt gaattcgatc acccagacca acccgatcaa
     1021 gtacttcaat tgcttttttt gcgggatttt taatacgttg atctctatcg tacattaacg
     1081 gaagttcaac attttcgagt gcagttgttc ttgcgagaag attaaaacct tgaaatacaa
     1141 atccgatttt ttgattcctg atttttgcgt aatcattttt tgaaagtgta cttgtattta
     1201 ctccatctag aaaataatca ccggaagttg gtttatccag acaacctatt agattcatca
     1261 aagttgattt acccgaacct gatgaaccca taattgcaac aaactcaccc tcatctattg
     1321 atagatcaac agattttaat gcttgtactt ctacttcacc tatcacataa gttttagtga
     1381 gtccaacaat tttcattatg tttttttctt taggcaaacc cattaaaatc ctcttcgggt
     1441 acttcgcggc atactttgat tttggtttaa aatattagtg ttttgtgtcg gcttattatc
     1501 agccactatt tcaaatcccg taataacctg catattttct tttaggtttc tgcttctaag
     1561 tatttcggtg tttttcccat cactaagacc caattgtgca aatcccattg ctggatttcc
     1621 attctcatca acataccaaa atgagcctct tgatttattg ccagaacttc caaaaccatt
     1681 tcctctattc tgcattccgc tgttgccgcc aaaagattga aatctttgtt ttaaactatc
     1741 ggacatattc ttaatttctt tttgcatctt agccgtaaaa tctgctttca tttgatcagt
     1801 tggttgaaac cttaaagctg aatttggaat catgagaaca ttagttcttt tctcaacata
     1861 aaaatccact gttgctgtca ttccaggtaa aagcaaatta tcatcgtttt cggcattaac
     1921 tacaactata taattaacaa cgttagatac aacgctagaa cccaatctta tttgaaatac
     1981 tttacctgta aattttttat cgggataggt ttgtacagta aattgagcgt cctgtccctc
     2041 tttaatctgt ccaatatcac tttcatcaac attagtaaga atctgcattt tggataaatc
     2101 ttctgcgata gtaaataaag ttggtgctga tagacttgct gcaactgttt ggccttcttc
     2161 tacatttcga tttataatct tgcctttaat tggcgaaaaa atgtaagcat agcttaaatt
     2221 agtttttgcc tgtgctaaag aagatttagc agaatttaga tttgctatcg ctgtttcgta
     2281 attagatttt gaggttataa aatcaagctc agacataaac tttttatcaa ataatgtctg
     2341 gtcacgttca tatgtcgctt tagcaacatc atacgaagct tgcgctttct gtaatcctgt
     2401 cagtgcgttg tttacctgca aagcaagatt agttgtatca agtacagcta acagctgtcc
     2461 tttccgtacg ttgttgttaa aatcaacaag cagtctatca atcttacctg aaacctgcgt
     2521 tccaacatct acagtagtta ctgcctgaag cgtacctgta cttgtaattg tagtgtttaa
     2581 atcacctctg gtaatagttg caaaagtaaa tttatttgtt cctgcttcac tgctgttaaa
     2641 gaacagaaaa taaattgcta ctaatgcaat tacaattata gaactgataa ttaagatttt
     2701 ctttttcatt tagacttcct gaaaggaaat aattgtttat aaaaagttta agtctctatt
     2761 aattttcttt gtttttattc tgctgatatt gtttttttct ctcttccaac attttattgt
     2821 actcaacttt ttgtttttca tttaacactt ttaaaatctc ctgatttgaa ttctccataa
     2881 tttttctaaa ttcatttctg tcaggattat cggaagatct taatttctgt atctgtttat
     2941 cattttctaa aagaattgat tcaactttta cagattgctc ttttgttaag ctaagttttt
     3001 cattcagtac ttttaatctt tcttgaggtg tgcgcatccc ttgtgcaaat gaagcgaaag
     3061 taaccataaa taaaaacaag cttgacatta aaattatctt tttcattttc gtaccttttt
     3121 gttttaaata ttttagacaa cttagattat ctaatagttt aattaatttt taaattttgt
     3181 atgaattaac ttaattagat gaaaatttgc tttaatatct ttaaatcaca caaaatgttt
     3241 taccagtatt aatatgcaac cttgtattgc ttctcacaac aatcttaata attcatttta
     3301 gagccgtgtc caggacaaac tttattatag aacaaagagg gaagaaaatc atagaaaaag
     3361 agcagggcat atacgccagc ggtattaaaa ttgaaagtga ataatgagag atagttaata
     3421 ctaattacat tgtaagattt agataacttg ttcattatag aaacaaaata ataacgtgca
     3481 ttaccaccca gataaagaaa taaagcgcta cctgttttcg caaacctttt gcaagatatc
     3541 caaaataaat acctttaata atagtattac tcgtcatcga tataataata gctgattcaa
     3601 ttatagttcc gactggttgc tgcttattaa tcagagagag aataaatgga tcaatatctg
     3661 taacgccgac tattccagat aagaccagta caccgccgcc gccgaaaaat tgttttaccc
     3721 aaagcgtaac tatagaaaga agaacaaaaa gaaaagcaaa aataaccgcc ggtcttattt
     3781 cgaaagggtt ttgaattgtt gaaataggct cagtagagat ctcaggattt ttatctctga
     3841 ttgtagcagc aagaattaga cccacaaaaa ataatatgca caatttccac caaattgcat
     3901 aagcaattcc cacgtttaac agatatataa ttataagtat tcttatatac ataacactgc
     3961 ttgctaagat tgttgcttga aatgcttgct tggcaatcag ctcatttttc tttgcaatgt
     4021 ttcccatagc tatactaaca gcagtgcttg atgcaatacc acccaacaat cccgataacc
     4081 agataccgac tttactgcca aattttttca tcaaaaaata tccaacaaaa ccgattgagg
     4141 atactaatat tactatctgc caaatcttat aaggatttaa attaaaccgg gtgtaatttt
     4201 gatcgggtaa agcaggaagt atgataatag taacgattaa aaatttaact actgcaagga
     4261 attctgattt atcaagtttt tcaatatact tttctaattc cgctttttct gatagcaaaa
     4321 gcgttgtgaa aatacctaat gccatgggaa tccagatatc tgcaagaagt gataaaacgc
     4381 cgactacaaa aatcagtaat gcagcaattt cactggtcca gccaacagca cctgattttt
     4441 gcttggcaag ataagaggcc agaatcattg caccgataac tactatccct accggaataa
     4501 taaattctat attcaacctg aaaagccagg cacacccaaa cccaaataaa ctgatcagtg
     4561 tgtacgttct gacaccagct gttagtttac tggaaagaga gccgcctctg ctttcacgtt
     4621 cgagtccaac caaaaatccc aatgctattg caacaagaaa tcttagctgg tcagtccatt
     4681 ctattaaatt catttaagta gaatcataat tatcgttagc aaatatatct aaagttattt
     4741 ttaattactt ttgctcagga aaaatatttt attctataat aatttttcct aaagtaagtc
     4801 tcgattaata aacaacaagt cataattatt agtgtgctgt aacatcacta attattatca
     4861 taattataag attgttaatt ttgtctgaag tttttcaagc agtaatcata aagggcatat
     4921 ttatattttt aatttttgtt atgacaattg tcgtttcttc aagtgcgttt tttcatccct
     4981 tttcacacat cgaagttgat aacccaaaca ttgaatacat aggcagattt gatttttcca
     5041 aagaaggtgt tgtaagattt gattggtcgg gagttcagat caagaccaag tttagcgggc
     5101 cttactgcgg aataaaactt aaagatggaa agaatgatta taatattttt attgatggaa
     5161 aactttacag aatattaaaa acaaattctg atactctcta tactttagcc gataatcttt
     5221 cagataatgt tcatacttta ttaattgcta aaagaactga agcaagaaat ggtttagttt
     5281 catttggagg attagtttta aattctggga aagggttatt gtctgtaagt aaagaaagta
     5341 aaagaaaaat tgagtttatt gg
//
LOCUS       DASWBY010000380         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_316079, whole genome shotgun sequence.
ACCESSION   DASWBY010000380 DASWBY010000000
VERSION     DASWBY010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316079"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1888
                     /locus_tag="VF870_06935"
     CDS             <1..1888
                     /locus_tag="VF870_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9251960.1"
                     /translation="KYSNKNFAAMLGYRINEIAGLSLNDVISPGDIDKVLERSDNRLR
                     GEAVPSQYEIDLIHKNKTKIIHVSISVGVIGSGTDKFQFVIVKDLSEKIEIESRYIRE
                     LQLQQYFMDYLPDSIYFKDLESRFIKANKATLIKMGLSSFEDLIGKTDFDIFNNEHAI
                     LARKDEEEIIKNRTSIINKIEKETWKDGKITWASTTKIPLMDENDNVQGTFGITRDIT
                     DLKKSEIIKDALFKISSAVTSQIKISELFEFIHKTITELMRAENFYIAMYHQDTDTVT
                     FPYWVDEVDPQPGEQKAERGLTEYVLRTGCAQLITEELDMKLREAGETSLLGEPTKIW
                     LGVPLKVEGKTIGVIVVQDYNDPLTYGEEEKEILTYISEQIALAIDKKYTEEKIIEYS
                     EELKELNAAKDKFFSIIAHDLKSPFHGLLGLTRMIVEEYDSMSESEVLQYLQIIKDST
                     ESTYKLIENLLEWSRLESGKMKFNPALQNMFMIVEDTRILLNQNARLKNVNIRNKLGH
                     QSFVWGDDTMLQSLIQNLISNAIKFTPPGGSIEIFENRSDEYIEFVVSDTGIGIKEKD
                     IEKLFRIDMNFTTKGTNQEKGTGLGLALCKEIINIHGGKIFVQSKVGKGTKIIFTLNK
                     PKNN"
     gene            1895..2233
                     /locus_tag="VF870_06940"
     CDS             1895..2233
                     /locus_tag="VF870_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251961.1"
                     /translation="MIEGTKNSGRDDLSEFADYLAEFDSLTNFDNETCPVCGVNISDS
                     LSIENSPFVQYKCLDEENEILVLTKELDLKCIPYKIEKRLNTEVNDKINYLFDILVPF
                     KFLSRLEKSI"
     gene            complement(2243..2650)
                     /locus_tag="VF870_06945"
     CDS             complement(2243..2650)
                     /locus_tag="VF870_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX9251962.1"
                     /translation="MDKKIRVVVAKSGLDGHDRGAKVIAAALRDAGMEVIYTGLRQTP
                     EMIVEAAMQEDADVIGISILSGAHMTIFPKLMTLMKEKGMNDVLLTGGGIIPEDDIVK
                     LKEIGVGELFTPGATTTAIADYIKEWCKAHPRN"
     gene            complement(2643..3074)
                     /locus_tag="VF870_06950"
     CDS             complement(2643..3074)
                     /locus_tag="VF870_06950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX9251963.1"
                     /translation="MDCIFCEIASKYSEAEIVFENDKFIAFLDINPVNYGHTLVVPKK
                     HFDNFLTIPANELEEMTKLTQYLSGAIKRALKADGFNVISNNGTTAGQSVFHFHYHII
                     PRFENDFHIKPRTIEYKHAEIEVYAEKIKEVVSKYEGLLNG"
BASE COUNT         1112 a          489 c          535 g          958 t
ORIGIN      
        1 taagtactcc aataaaaact ttgccgctat gctgggttat agaataaatg aaattgccgg
       61 attaagttta aatgatgtaa tatcccctgg agatattgat aaggttcttg aaagaagcga
      121 taatcgtcta agaggtgaag cagttccttc ccaatatgaa atcgatctaa ttcataaaaa
      181 taaaacaaag attatccatg tttctattag cgttggagtt ataggttcag gaacggataa
      241 gtttcagttt gttattgtaa aagatttatc tgaaaaaatt gaaatagaat caagatatat
      301 ccgggaactt cagctgcagc aatattttat ggactatctg cctgattcaa tatactttaa
      361 ggatcttgaa agtagattca taaaagcaaa taaagcaaca ctaattaaaa tgggcttaag
      421 ttcattcgaa gatctgattg gaaaaactga ttttgatatt tttaataatg aacatgcaat
      481 acttgccaga aaggacgaag aagaaataat taaaaaccgg acttctataa tcaataagat
      541 cgagaaagaa acatggaagg atggtaaaat aacatgggca agtacaacca agattccgtt
      601 aatggatgag aacgacaacg tccagggtac atttggaatt accagagaca ttaccgattt
      661 aaagaaatca gaaattatta aagatgcttt atttaaaatt tcatcagcag ttacatctca
      721 aataaaaatc agtgagctat ttgaatttat acataaaacg ataactgaat tgatgcgtgc
      781 agaaaatttt tatattgcta tgtatcatca ggacacagat acagtaacat tcccatactg
      841 ggttgatgag gttgatccac aaccaggtga acaaaaagct gaaagaggtt taactgaata
      901 cgttttacga acaggctgtg ctcaattaat tactgaagaa ctggatatga aattaaggga
      961 agcgggtgaa acatctttac tcggggagcc aacaaaaatt tggctaggcg tacccttaaa
     1021 agttgaagga aaaacaattg gagttatcgt agttcaggat tacaatgatc cccttaccta
     1081 tggagaggaa gagaaagaaa tactaactta catttctgaa cagatcgcac ttgcaataga
     1141 taaaaagtac acggaagaaa aaattattga atattctgaa gaactcaaag aactaaatgc
     1201 tgctaaggat aagttctttt ctatcattgc tcacgattta aaaagtccct tccatggatt
     1261 gcttggtctt actcgtatga ttgttgaaga atatgattca atgagtgaaa gtgaggtgct
     1321 tcaatatctg caaataatca aagattctac tgaaagcact tataaactga ttgaaaacct
     1381 gcttgaatgg tcaaggttgg aatcgggtaa aatgaaattc aatcccgcac tgcagaacat
     1441 gtttatgatt gttgaagata caaggattct gctaaatcaa aatgcacgat tgaaaaatgt
     1501 taatatacgg aataaacttg ggcatcaaag ttttgtttgg ggagatgaca caatgcttca
     1561 atctcttata cagaatctaa tatcaaatgc aataaagttt actcctcctg gaggcagtat
     1621 tgagatattt gaaaacagat ctgatgaata catagagttt gttgtaagtg atacaggaat
     1681 aggtattaaa gaaaaagata tagaaaaact ctttagaatt gatatgaatt ttacaacgaa
     1741 aggaaccaat caagaaaaag gcacagggct gggattagca ctttgtaaag aaattattaa
     1801 tattcacggt ggtaaaattt tcgtgcaaag caaagtcggc aaaggaacaa aaataatttt
     1861 tacattaaac aaaccgaaaa acaattaaac ttctatgatc gagggcacta agaattccgg
     1921 gagagacgat ctttcagaat tcgcagatta tttagcagag tttgattcat taactaactt
     1981 tgataatgaa acctgtccgg tttgcggtgt taacatatca gattcacttt caattgaaaa
     2041 ttctcctttc gtacaataca aatgtttaga tgaagagaat gaaattttag ttttaaccaa
     2101 ggaattagat cttaaatgta ttccatataa aattgagaaa agacttaata cagaagtgaa
     2161 tgataaaatt aattatcttt ttgatattct cgttcctttt aaatttttat cccgcttaga
     2221 aaagagtatc tgaactaatt aatcaatttc ttggatgagc tttgcaccac tcttttatat
     2281 aatctgcaat tgctgttgtg gttgcaccag gagtaaataa ttcaccaact ccaatttctt
     2341 ttaactttac aatatcgtct tcaggtataa ttccaccgcc tgtaagcagt acatcattca
     2401 tccctttttc tttcatcaaa gtcataagtt ttggaaagat tgtcatatga gcaccagata
     2461 aaatactaat accaattaca tcagcatcct cctgcattgc agcttcaact atcatttcag
     2521 gagtctgtct taagcctgta taaataactt ccattcctgc gtcgcgtaaa gctgcagcta
     2581 taacttttgc acctctgtcg tgtccatcta aacctgattt agcaacaaca actcttattt
     2641 ttttatccat tcaacagacc ctcatatttt gaaacaactt ctttaatctt ttcagcataa
     2701 acttcaatct ctgcgtgctt atattcaatc gttctaggtt taatatgaaa atcattttca
     2761 aatcttggaa ttatatgata atgaaaatga aaaacagatt gcccagcggt agttccgttg
     2821 tttgaaatta cattgaatcc gtcagccttc aatgctcttt taatagcacc tgaaagatat
     2881 tgtgtcagct ttgtcatttc ttcaagttca ttggcaggga tagttaaaaa gttatcaaaa
     2941 tgttttttag gaacaacaag tgtgtgtccg taattaactg gatttatatc aagaaaggct
     3001 atgaatttat cattttcaaa aacgatttcg gcttcagagt atttcgaagc aatttcacaa
     3061 aatatacaat ccatattatg ctcgctttat attt
//
LOCUS       DASWBY010000381         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_579112, whole genome shotgun sequence.
ACCESSION   DASWBY010000381 DASWBY010000000
VERSION     DASWBY010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579112"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VF870_06955"
     CDS             <1..499
                     /locus_tag="VF870_06955"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX9251964.1"
                     /translation="SKVLVLNQSYEPLSVCNIRKAVILLYLGKAEPILNDDRKALHSV
                     GKNYPWPSVIRISKFVKLPYKKVVLTRKNILRRDAYKCAYCGRSDIALTVDHVFPRGR
                     GGDETWENLITACTNCNNRKGDRTPEEANMKMLFRPFKPSHILFIKNVVGRLDERWKP
                     YLYLS"
     gene            557..1537
                     /gene="trpS"
                     /locus_tag="VF870_06960"
     CDS             557..1537
                     /gene="trpS"
                     /locus_tag="VF870_06960"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX9251965.1"
                     /translation="MPKKRILSGMRPTGKLHLGHYVGALENWVKLQNEYESFHLIADY
                     HVLTTSLDTTDIYQNSIDMLIDWLACGLDPAKSPMFRQSQIKQHTELNLIFSMLITVN
                     RLERNPTLKDQIRDLNIEHVIYGHLGYPVLQTADILLYKGDAVPVGDDQVPHIEISRE
                     IARRFNNQYGEVFPEPDPLLTKFSRLSGLDGNAKMSKSLGNTILLSDDPETVKARMKK
                     AAIDPNKQRKGDPGNPEICVVFSYHKKFNPDEIPEIDAGCRSGALGCFDCKMNCAAKI
                     SKFLKPIIEKRKYYESRIDEVKNILHDGETKGRKVAEETMHQVHDKMKLG"
     gene            1537..2256
                     /locus_tag="VF870_06965"
     CDS             1537..2256
                     /locus_tag="VF870_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX9251966.1"
                     /translation="MYKIKLDQFEGPLDLLLFFIKRDELNIYDIPISRITGEFLEYVN
                     LIKLMDLEVAGDFILMASTLMHIKVRMLLPRELDERGEEIDPRADLIKALLEYKKYKE
                     VAEDLTYFESSQRKTSFRGDFSQDAMESPPEYEILLKNISIYDLAKAFKRAIEGIKPQ
                     PVHEVKKINVSIDEQIQYILSRVIEKGEIHFLSLVHGMKEKIRIVITFVALLELTKME
                     KIGIKESPDFNDFVIYGKSNG"
     gene            2249..>2857
                     /gene="scpB"
                     /locus_tag="VF870_06970"
     CDS             2249..>2857
                     /gene="scpB"
                     /locus_tag="VF870_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX9251967.1"
                     /translation="MDKTYTSIIEALIFSSDEPISENEIIRAIKGIDGEEIEINSSDV
                     HSAVEELNNKYEEHGNSFKVKKIANGFIFATTEINAKYVGFLSSEKSKRRLSQAALET
                     LAIIAYKQPITKPELEQIRGVNSDYILNTLLEKNLITITGRAETIGRPLLYGTTTEFL
                     KYFGLYNLSDLPKPREIEEIMKDEDFIEQKNKIMMNLVEETLE"
BASE COUNT         1029 a          426 c          546 g          856 t
ORIGIN      
        1 ttctaaagtt ctggttttaa atcaaagcta cgaaccactt tcagtttgca atatccgcaa
       61 ggctgtaatt ttactttatc ttggcaaagc agaaccaatc ctaaatgacg atagaaaagc
      121 attacactcg gtaggaaaaa attatccctg gccaagtgta attcgaatca gcaagttcgt
      181 aaagttgcca tataaaaaag tagtactaac cagaaagaat attctcagaa gagatgctta
      241 caagtgcgca tactgtggaa gatctgatat cgcattaact gtcgatcatg ttttcccaag
      301 aggacgaggc ggagacgaga cgtgggagaa tcttattaca gcttgtacaa attgtaataa
      361 tcgtaaaggc gatcgaactc ctgaagaagc gaatatgaaa atgttattca gaccatttaa
      421 accaagccat attttattta ttaaaaatgt ggttggtagg ctggatgagc gctggaaacc
      481 ttatctttat ctatcctgaa aattacacgc tttattatta tctttcgatt gaatttttta
      541 acaatattta tattaaatgc ctaaaaaacg aatattaagc ggaatgcggc cgaccggtaa
      601 attgcatctt ggtcactatg ttggtgctct ggaaaattgg gtaaaactgc aaaatgaata
      661 tgaaagtttt catcttattg ctgattatca cgttttaacc acaagtttgg atacgactga
      721 tatatatcaa aattctatag atatgctaat agattggtta gcttgtggtt tggatcctgc
      781 taagagtcca atgtttagac aatctcaaat taaacagcat actgaattga atttgatttt
      841 tagcatgctg attactgtta acagacttga acgtaatcct actttaaagg atcaaatcag
      901 agatttaaat attgaacacg ttatttacgg acatttaggt tacccagttt tgcagacggc
      961 tgacatattg ttgtataaag gtgatgcagt gcctgttggt gatgatcagg ttccacatat
     1021 tgaaatatcc agagagattg caagaagatt caataatcaa tacggagaag tatttccgga
     1081 acccgatcca ttgcttacaa aattctccag gctttcgggt ttagatggaa atgcaaagat
     1141 gagtaagtca ttgggaaata caattcttct ttctgatgat cctgaaactg taaaagccag
     1201 aatgaaaaaa gcagctattg atcctaataa gcaaaggaag ggcgatcctg gaaatccgga
     1261 gatatgtgtt gtcttttcat atcataaaaa attcaatcct gatgaaatac cagaaattga
     1321 tgccggctgc cgttcaggtg ccctcggttg ttttgattgt aagatgaatt gtgcagcaaa
     1381 gatttctaaa tttttaaagc caatcattga aaaacgaaaa tattatgaga gccgaataga
     1441 tgaggtaaaa aatattctgc atgatggcga aaccaagggt agaaaagttg ctgaagaaac
     1501 tatgcatcaa gttcatgata aaatgaagtt aggttaatgt ataaaataaa attagatcag
     1561 tttgaaggcc cattagattt acttctcttt tttattaaaa gagatgaatt gaatatttat
     1621 gatattccta tctcacgaat tacaggcgaa tttttggaat acgtaaatct tattaagctg
     1681 atggatcttg aagttgccgg tgattttatt ttgatggcat caacattaat gcacattaaa
     1741 gttaggatgc tattaccacg tgaacttgat gagcgaggag aagagattga tccaagggct
     1801 gatcttatta aagctttgct tgaatataaa aagtacaaag aagttgctga agatcttaca
     1861 tactttgaat caagtcagag aaaaacaagt tttcgaggcg atttctcaca agatgcaatg
     1921 gaatctcctc cggagtatga aatacttcta aagaatattt ctatttatga tcttgcaaag
     1981 gcttttaaga gagctatcga aggaattaag ccacagccgg ttcacgaagt taaaaagata
     2041 aacgtatcta tagatgaaca aatccagtac attttaagtc gtgttattga aaaaggcgaa
     2101 atacattttc taagtttagt acatggaatg aaagagaaga taagaattgt gataaccttt
     2161 gttgcactac ttgaattaac taaaatggaa aaaataggaa ttaaagaatc accggatttt
     2221 aatgattttg taatttacgg taaatcaaat ggataaaact tatacatcaa taattgaagc
     2281 attaatattt tcttcggacg aaccgatttc tgaaaatgaa attattcgtg ctataaaagg
     2341 aatagacgga gaagaaatag aaatcaattc ttccgatgtt cattctgcag ttgaagaact
     2401 aaacaataaa tatgaagaac acggtaattc gtttaaagtt aagaaaattg caaatggatt
     2461 tattttcgct acaacggaaa taaatgcaaa gtatgttggg tttctgtctt cagaaaaatc
     2521 taaaagaaga ttaagccagg ccgccctgga aactcttgca ataattgcat acaaacagcc
     2581 gataacaaaa cctgagttag aacaaatccg tggtgttaat tcagattata ttcttaatac
     2641 actgctagaa aagaatctta taacaataac cggtagagca gaaaccattg gcagaccttt
     2701 gctttatgga accacaacag agttcttaaa atattttggg ttgtataatc ttagcgacct
     2761 tcctaaacca cgtgagattg aagagataat gaaagatgaa gattttatag aacaaaaaaa
     2821 taaaataatg atgaatctgg ttgaagaaac tcttgaa
//
LOCUS       DASWBY010000382         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_468657, whole genome shotgun sequence.
ACCESSION   DASWBY010000382 DASWBY010000000
VERSION     DASWBY010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468657"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VF870_06975"
     CDS             <1..1156
                     /locus_tag="VF870_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9251968.1"
                     /translation="EAERFEREGNLGKVAEIRYGRITELEKKLKEETQRLAEAQKDKK
                     MLKEEVDAEDIAEIVSKWTGIPVNKMLESERSKLLRLEDELHQRVIGQDEAVSAVSNA
                     IRRSRSGLQDANRPIGSFIFLGTTGVGKTELARALAEVLFDDEHAMIRIDMSEYMEKF
                     SVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLLDEIEKAHTDVFNVLLQVLDDGRL
                     TDNQGRTVNFKNTIIIMTSNLGSHLIQDRLEAFDENNVESFMGELRQQMHDLLRKTIR
                     PEFLNRIDEVVLFKPLSRIEIREIVDLQLTRVEKMLKVKEITLDVSDEAKDWLAQLGY
                     DVTYGARPLKRVIQKYLVNPLSQELLAGNFGDGDTIKISVGESAKLVFTK"
     gene            1343..>1615
                     /locus_tag="VF870_06980"
     CDS             1343..>1615
                     /locus_tag="VF870_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9251969.1"
                     /translation="MTTWKIDAAHSEVNFKVKHMLVSTVRGNFDNFDATIETNKEDFS
                     DAVIKFEADVNTINTNNEQRDTHLKSADFFDAENHPKMIFESKSIKK"
BASE COUNT          565 a          251 c          339 g          460 t
ORIGIN      
        1 agaagctgaa agatttgagc gtgaaggtaa tcttggcaaa gttgcagaaa tccgttatgg
       61 aagaataact gagcttgaaa agaaattaaa agaagaaaca caaaggcttg ccgaagcaca
      121 aaaagataaa aagatgctta aggaagaagt tgatgcagaa gatattgccg agatagtttc
      181 aaagtggacc ggaattcctg ttaacaaaat gcttgaaagt gaaagaagta aacttttaag
      241 acttgaagat gaacttcatc aacgtgttat tggacaggat gaggctgtat ctgcagtttc
      301 aaatgcaatt cgaagatcac gctcaggttt gcaggatgca aatcgcccga ttggttcatt
      361 cattttcctt ggaacaacag gtgttgggaa gactgaactt gcacgagctt tggctgaagt
      421 tttatttgac gatgaacatg caatgatcag aattgatatg agtgagtata tggaaaagtt
      481 ttctgtatct cgtttgatcg gtgctcctcc cggatatgtc ggatacgagg aaggcggtca
      541 attaactgaa gccgtccgaa gaagacccta ttcggtcgtg ctgctcgatg aaattgaaaa
      601 agcacatacc gatgttttta atgttctgct tcaggtttta gatgacggaa gattaactga
      661 taatcaaggt agaactgtga actttaaaaa cacaatcatt ataatgacat ctaatcttgg
      721 ctctcattta atacaggata ggcttgaagc ttttgatgaa aacaatgtcg aatcttttat
      781 gggtgagctt agacagcaaa tgcacgactt gcttagaaaa acaattcgtc cagagttttt
      841 aaatagaatt gatgaagttg ttctgtttaa accattatca agaatagaga ttagagagat
      901 tgttgattta caacttactc gtgtagaaaa aatgttaaag gttaaagaaa ttactcttga
      961 tgtttcagat gaggctaagg actggctggc tcagcttgga tatgatgtga cttacggtgc
     1021 acggccgctg aagagagtaa ttcaaaaata tcttgtaaat cctctttcac aggaattact
     1081 cgccggtaat tttggtgatg gtgatacaat taaaatctct gttggggaga gtgccaaatt
     1141 agtatttaca aaataatgtc acactgagcg aagtcgaaac gtgacccgat tagttaaccg
     1201 caggaaaaaa cttctgcggt tttttagttt tttccactct ttctatcggt tttcttggaa
     1261 agaatatcca agtgaacttt tctgctcaaa tatgtgttat aacatataga acaacaaaaa
     1321 aactaaatga aaggatacta aaatgacaac atggaaaatt gacgcagcac actctgaagt
     1381 taactttaaa gtaaaacata tgctggtttc aactgtaaga ggaaactttg ataatttcga
     1441 tgcaacaatc gaaacaaata aagaagactt cagcgatgca gtaattaaat ttgaagctga
     1501 tgtaaacacc atcaacacaa ataacgaaca gcgtgatact cacttaaaat ctgctgattt
     1561 ctttgatgca gaaaatcacc caaagatgat ttttgaatca aaatctatta agaaa
//
LOCUS       DASWBY010000383         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_351594, whole genome shotgun sequence.
ACCESSION   DASWBY010000383 DASWBY010000000
VERSION     DASWBY010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351594"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VF870_06985"
     CDS             <1..1146
                     /locus_tag="VF870_06985"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9251970.1"
                     /translation="IIVLGDGTIKFREIVEHYYAGLSFDTISGLAIVSGKNKILRTPK
                     SDYMTDPDKLPFPRRDLLLHISHKYYYLFHQPVTLMKTTWGCWYKCNFCFNWKVTCGI
                     PYTRSPESIVKEIETIPTNDVYIVDDIFLINRKRLLNIAELIKQKKINKKYLVYSRAD
                     FICENEDVIKIWSEVGLSAVIVGLEATTDPELDSMNKQCTVDYNRKAIEILKRYRIDT
                     YASLIPLPGYSKADWDRLLNFIFETGLYYVNISPLTPMPGTDIWENYKDQITISTKAH
                     GLWDLSHTVLPTKMDLRDFYKNLLRVYSKTILDVKRAGKLTLRTRPPILSITYIRLLS
                     GAFRIALQFLTCYRHHLPKSIIKAEYQGPDTKELPMPVFNSSKAGMI"
     gene            1146..>1595
                     /locus_tag="VF870_06990"
     CDS             1146..>1595
                     /locus_tag="VF870_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251971.1"
                     /translation="MINFQTKYLSFDSKQVRLQSNHIKSDLSLCTYNSICEIDKQFWD
                     NLSNDVFNSWDFLLSLEKARVENSQMKYLIFFHNSTPIAIAVLSMFSISLDLLSGQFL
                     SKICSTFRKIFPGFMKLKFLFCGTPISIGKNNLRIIDESKRNQILNLL"
BASE COUNT          560 a          244 c          258 g          533 t
ORIGIN      
        1 attattgtac ttggtgatgg cacaattaag tttagagaaa tagtagaaca ttattatgcg
       61 ggactatcat ttgatacaat atcgggttta gcaattgtaa gcggaaaaaa taaaatcttg
      121 aggacgccta aatcggatta tatgacagat cctgataaat taccttttcc aagaagagat
      181 ttacttttac acatttcaca taaatattat tatttatttc atcagcctgt aacattaatg
      241 aagacaactt ggggctgctg gtataaatgt aatttttgct tcaactggaa agtaacttgc
      301 ggaattccat atacacgctc ccccgaatca atagtaaaag aaattgaaac cataccaaca
      361 aatgatgtat acatagttga tgatattttt ttaataaatc gtaagagatt actaaatatt
      421 gcagaactta taaaacaaaa aaaaattaat aaaaaatact tggtttattc acgagctgat
      481 tttatttgtg aaaatgaaga tgttataaaa atatggtctg aagttggttt gagcgctgta
      541 atcgttggtc ttgaagcaac aactgatccg gaacttgatt ctatgaacaa gcaatgtaca
      601 gttgattata acagaaaagc tattgaaatt cttaaacgat acagaattga tacgtatgca
      661 tccttaattc ctttgcccgg ttatagtaaa gctgattggg atagattact taattttatc
      721 ttcgaaactg gattatacta cgttaatatt tcacctttaa caccaatgcc gggtactgat
      781 atctgggaga attataaaga tcagataact ataagtacaa aagctcacgg tctatgggat
      841 ctttctcaca cagttcttcc aaccaaaatg gatttaaggg atttttacaa gaatttactg
      901 agggtttaca gcaaaactat attggatgtt aaaagagccg gcaaattaac tttaaggacc
      961 cggcctccta ttttatcaat tacatatata agacttttat ctggtgcttt tagaattgca
     1021 ttgcaattcc tcacttgtta ccgacatcat ttacccaaaa gtattataaa ggcagaatat
     1081 caaggaccgg ataccaaaga attgcctatg ccggtattta attcttctaa agctggaatg
     1141 atttaatgat taattttcag acaaaatacc tttcttttga cagcaaacaa gtaaggttgc
     1201 agagtaacca tataaaatca gatctttctc tctgcacata taattccata tgtgaaattg
     1261 ataagcaatt ttgggataat ctttcaaatg atgttttcaa ttcatgggat tttcttttaa
     1321 gtcttgaaaa agccagggta gaaaatagtc aaatgaaata tttaatcttt ttccacaatt
     1381 ctaccccaat agcaattgct gtgttatcta tgttttcaat ttcactggat ttattgagtg
     1441 gtcaattcct ttcaaagata tgcagtacct ttagaaaaat ttttccggga tttatgaagt
     1501 taaaatttct tttttgcgga acaccaattt ccatcggaaa aaataatttg cgaattattg
     1561 atgagtcaaa gagaaatcaa attctaaatt tatta
//
LOCUS       DASWBY010000384         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_241298, whole genome shotgun sequence.
ACCESSION   DASWBY010000384 DASWBY010000000
VERSION     DASWBY010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241298"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VF870_06995"
     CDS             <1..560
                     /locus_tag="VF870_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX9251972.1"
                     /translation="LGGAGSSGTEGTAFDEVLEKAEPQEKDITKNKNLEVKEVELPKA
                     KNTDVSDVAAAQKDKEVKKETVKQTEQRDSENLTGDKKGNLNQGTGDFGFHFEGGGLG
                     TRKIYSYVIPAYPEGVNKEIDIRLKFTIKPDGTVGSIFLLSKADTRLENAAINSLWQW
                     RFEPLSANQVQGDQTAVIVFPYRLQ"
     gene            complement(532..831)
                     /locus_tag="VF870_07000"
     CDS             complement(532..831)
                     /locus_tag="VF870_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251973.1"
                     /translation="MITAFIFFVHLIFGLVIFTKKWQDENLSAGFLNITLILILFAVG
                     WAITGIIAKLVMEPKGLGMQFDRDTFSLTLLTIIEFFFYKFYYQDDPTEADKEKQ"
     gene            complement(828..1346)
                     /locus_tag="VF870_07005"
     CDS             complement(828..1346)
                     /locus_tag="VF870_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251974.1"
                     /translation="MNFKKYLPTLVCGFAAAVLATVPGIKNFSCCLIVPGAATISILL
                     DKKINNYNEKVVIGKSLGFGFLTGLFATLFITTFEMLVTYFTRSNDFIQSLPQTELLM
                     RQWNLGPLVDESLKLIKSVSKEIQKSGFSFLYLLMILSSNFMINSIFGMIGGALGMSL
                     ANRKSREDDHQV"
     gene            complement(1336..>1589)
                     /locus_tag="VF870_07010"
     CDS             complement(1336..>1589)
                     /locus_tag="VF870_07010"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEX9251975.1"
                     /translation="YAGTLISAFILMFYSKQFIFKTKYLIMLSLPMLLDVILLNLGAY
                     NYNHLISALTGLLFGSSVFLYILSAIENLLFNKPNNNEL"
BASE COUNT          587 a          243 c          292 g          467 t
ORIGIN      
        1 ttttaggcgg tgcaggttca tcgggtacgg aaggaacagc gtttgatgaa gtattagaaa
       61 aagcagagcc tcaggaaaaa gatataacaa aaaataagaa ccttgaggtg aaagaggttg
      121 aacttccaaa agcaaagaat acagatgtta gtgatgttgc agctgctcaa aaagataaag
      181 aagtaaaaaa agaaactgta aaacaaactg aacagcgtga tagtgaaaac ctaaccggtg
      241 ataaaaaggg aaatcttaat cagggtaccg gagattttgg attccatttt gagggcgggg
      301 gattaggtac aagaaaaatt tacagctatg ttattcctgc ctatccggaa ggtgtaaata
      361 aggaaattga tataagatta aagttcacga ttaaacctga tggcaccgtc ggttcaatat
      421 ttttactttc gaaagcagat acacgattag aaaatgcagc cataaattca ttatggcagt
      481 ggaggtttga acctttaagt gcaaatcagg ttcagggtga tcaaactgca gttattgttt
      541 ttccttatcg gcttcagtag gatcatcctg ataatagaat ttataaaaga aaaattctat
      601 tatggtaagc agtgttaatg aaaaagtatc cctgtcaaac tgcattccta agcccttcgg
      661 ttccataaca agttttgcaa ttattcctgt aattgcccat ccgactgcaa ataatattaa
      721 aattagtgtg atgttcaaaa atccggctga tagattttca tcctgccatt tctttgtaaa
      781 gattactaat ccaaaaataa gatgaacgaa aaagatgaat gctgtaatca tacttgatga
      841 tcatcctctc gtgattttcg gttagccagg ctcataccaa gtgcaccacc aatcattcca
      901 aaaatagaat taatcataaa gttacttgaa agaatcatta gcagataaag aaatgaaaag
      961 ccgctcttct gaatttcttt tgaaacactt tttatcagct ttaaagattc atcaactaat
     1021 ggtcctaaat tccattggcg cattagtaac tctgtttgtg gaagcgattg gataaaatca
     1081 tttgaccttg taaaataagt tacaagcatt tcaaatgtgg taataaacag agtagcaaat
     1141 aaacctgtta aaaaaccaaa tcctaatgat tttcctataa caactttttc attgtaatta
     1201 ttaatttttt tatcaagcaa aatagatatt gtagcggcac cagggactat aaggcaacag
     1261 ctgaagtttt ttattccggg aactgttgca agaacagcag cagcaaaacc acacactaaa
     1321 gttggaagat attttttaaa gttcattatt gtttggcttg ttaaaaagta aattttcaat
     1381 tgcacttaaa atatatagaa atactgagga accgaaaagt aaacctgtta atgctgatat
     1441 taaatgatta taattataag cccctagatt cagtagaatg acatccaaaa gcattggcaa
     1501 tgaaagcatt atcaaatatt tagttttaaa gataaattgt tttgaataaa acataagtat
     1561 gaaggcggaa attaaagtgc ctgcgtata
//
LOCUS       DASWBY010000385         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_540654, whole genome shotgun sequence.
ACCESSION   DASWBY010000385 DASWBY010000000
VERSION     DASWBY010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540654"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2256
                     /locus_tag="VF870_07015"
     CDS             <1..>2256
                     /locus_tag="VF870_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9251976.1"
                     /translation="DPLAGTGFGLQLSANAIEEVEVITGGFNAEFGQATSGIVNVKTR
                     EGGDKYSGSISYKRDYLGDKSSYHVFNTDIIEGNLSGPEPITSLILPALGLKIPGELI
                     FFGSVYTGLTDGITQGYYKPTANQLNSSIFNGTRFAPKAENNWFWLAKLTYKYSPTLK
                     FYYSFNQSVNINQNSQSLQSNLEYVEPSPGYQYNFQNILDNANTYTHNNKIHSLGVTH
                     TLSSKTFYELKVNSLFTNLRADANGNDWYLYNEPKDIVNFPIEYYNLNRDTVGVIPGD
                     GFYDVGNPFTWHDHYFQEFSVRGDITNFFDEKNKFKAGFDFKFQEMQFIDIYRPWIGE
                     LGLNNDIYKVYPAVGALYAQDNINFSGMILNFGLRMDYWFPGKYVDDAVNNPDVVTIP
                     DEVREAYYEDTYAWFGDRRFKARLSPRLGISHPVSDNQTLFFSYGHFSKWPKPQFVYA
                     KLDPLNAQSSFQRFGNPNLNPETTVAYELGVKTQFTSDDVFTITAYYKDIFDYINTRT
                     AKIVSPRFSTQSFITYVNSDYARSRGVELEYRKRIGKWFNGVASFSYAIVTGKSSSAD
                     EGVLVLQGDLNESIKEEYVPWDRPITASLSTSFYVQQGEPLFGFAPGVLDDYNLYLRF
                     FFQSGKRYTPAIFTGYVDEEGRAEYETDFNNRNSKIGEDWFWVDLNFEKYFQLMNLKF
                     SLFVEVNNLLDRQNSAIINPVTGRAYEYGDDLPASLNDPRYPDLQAPITPYPYNPARY
                     LTRRNIKFGVSL"
BASE COUNT          736 a          364 c          417 g          739 t
ORIGIN      
        1 gatccgcttg caggcacagg gtttggttta cagctctcag caaatgcaat tgaagaagtt
       61 gaagttatta ccggtggatt caacgcagag tttggacaag ccacttctgg aattgtaaat
      121 gtaaaaacca gagaaggtgg agataaatat tccggttcta tttcttacaa aagagattat
      181 ctcggtgata aatcttccta tcacgttttt aatactgata ttattgaagg aaatcttagc
      241 ggccctgaac caattacctc tttaatttta ccggcgttgg gattaaagat acccggtgaa
      301 ttaattttct ttggaagtgt ttatacagga ctaactgatg gcataactca aggctattat
      361 aaacctacag caaaccaatt gaattcatca atatttaacg ggacaagatt tgctccaaaa
      421 gcagaaaaca attggttctg gcttgcaaaa ctaacttaca agtattcacc aactttaaaa
      481 ttttattact cctttaatca atcggttaac atcaatcaaa attcgcaatc cctgcagtca
      541 aaccttgagt atgttgagcc aagtcctggt tatcagtata atttccaaaa catattagat
      601 aatgctaata catatactca taacaataaa attcattcat taggtgtaac gcacacttta
      661 agttcaaaaa cattttatga actaaaagta aacagtttgt ttacaaacct gcgtgcagat
      721 gcaaatggaa atgattggta tttgtacaac gaacctaaag atatcgtaaa ctttccaatt
      781 gaatattata acctgaacag agacacggta ggagttattc ctggtgatgg cttttatgat
      841 gtaggaaatc catttacctg gcatgatcat tattttcagg aattttcggt tcggggtgat
      901 atcactaatt tttttgatga gaaaaataaa ttcaaagcag gttttgattt taagtttcag
      961 gaaatgcagt ttattgatat ttatagaccc tggattggtg agcttggttt gaataatgat
     1021 atttataaag tttatcctgc agttggtgct ctttatgctc aagacaatat aaattttagt
     1081 ggaatgatcc tgaactttgg attacgtatg gattattggt tcccgggaaa atatgttgat
     1141 gatgctgtaa ataatcccga tgttgtaact attcctgatg aggttcgaga agcttattat
     1201 gaagatacat atgcctggtt tggagacaga agatttaaag caagattgag tccacgactt
     1261 ggcatttcac atcctgtttc tgataaccag acattatttt tctcctatgg gcattttagt
     1321 aagtggccaa aacctcaatt tgtttatgct aaattagatc cgttaaatgc acaatcatca
     1381 ttccaaagat ttggtaatcc taacttaaat cctgagacaa ctgtagcata tgaacttggt
     1441 gtaaaaactc aatttacttc ggatgatgtt tttactataa cagcttatta taaagatatt
     1501 tttgattaca taaataccag gacagcaaag attgtatcac caagattttc aacacaaagt
     1561 tttataacat atgtaaattc agattatgcg cgctctcgtg gtgttgaact tgagtatcgt
     1621 aaacgaattg gaaaatggtt taatggcgta gcttcattta gttatgcaat cgttacaggt
     1681 aaaagttctt ctgccgatga aggcgtatta gttttgcaag gtgatcttaa tgaatcaata
     1741 aaagaagaat atgttccgtg ggatagacca attacagcgt cgctttcaac aagcttttat
     1801 gtccagcaag gtgaaccgct atttggtttt gcaccaggcg ttttagatga ttacaatctt
     1861 tacttaagat tctttttcca atcaggtaaa agatacacac ctgccatctt tactggttat
     1921 gttgatgagg aaggaagagc tgaatatgaa actgatttta ataacagaaa cagcaaaatt
     1981 ggagaagact ggttctgggt ggatttaaat tttgaaaagt attttcaatt aatgaatcta
     2041 aaattttctc tttttgttga agtaaataac cttcttgata gacaaaactc cgccattatt
     2101 aacccggtta caggcagagc atatgagtat ggtgatgatt tgccggcaag cttaaatgat
     2161 ccacgttatc cggatttgca agcacctatt actccttatc catataatcc tgcaagatat
     2221 ttaactcgta gaaatataaa atttggagtg agctta
//
LOCUS       DASWBY010000386         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_729803, whole genome shotgun sequence.
ACCESSION   DASWBY010000386 DASWBY010000000
VERSION     DASWBY010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729803"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /locus_tag="VF870_07020"
     CDS             <1..1642
                     /locus_tag="VF870_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9251977.1"
                     /translation="DEEQTGKGNLLQRSLFKFNHWFDRLNGRYEKALRWSLTHRKTIV
                     FGAIAIFILSFMLMGMLGSQFFPDSDQSQFNLVVEAAPGSSLEQTSLICEKAEAVIRK
                     NPEVKTILTTIGSGNDPVTKATILVKLVDTKDRKRSDKKLMSEIRKELKYIPGAKFSM
                     RVQGGPGGNEKPITLSIRGEDITEIQKIADKVEAIVKTTPGAVDVDNSLELSKPEIRI
                     NIDREKASDLAVNPFLIASTIRAMVDGAVATQFKEGDEQIDVRVRLRKDNRRNINDLS
                     LITVKSNKKIGNQDFLVPITDIVTITKDVGPSKINRYARQKEIRVDANLDGVFLGVAL
                     GKILEQTSKLELKPGYSINVIGQGEMQDESMANMLITLLLAVVFVYIVLAAQFDSFIH
                     PFSIMLALPMSIIGAVLALLIFGSSLSVMSMIGIIMLMGLVTKNGILLVDFTNVLRER
                     GMSRFDALVKAGPTRLRPILMTTFAMIFGMVPVALALGEGAEFRAPMGQAVIGGLITS
                     TLLTLFIVPVVYSLLDDLGTFSFSGFFKKLFSRRKKQN"
     gene            1651..1950
                     /locus_tag="VF870_07025"
     CDS             1651..1950
                     /locus_tag="VF870_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251978.1"
                     /translation="MKLTFLVYHDILDDRVTEALKGLEIDFYTQWEDVKGKGHNTDAH
                     LGNRPFPGFNFVRMIAFTEEDLLEKLIAEINELNKIVERDDDKIRLFQVPLERIV"
BASE COUNT          666 a          334 c          429 g          589 t
ORIGIN      
        1 agatgaagaa cagacaggta aagggaactt actacagcga tctttattta agttcaatca
       61 ctggtttgac agactgaacg gcaggtatga aaaagcatta cgatggtcgt taacccacag
      121 aaaaactatt gtatttggtg caattgcaat ttttatttta agttttatgc ttatgggtat
      181 gctcggctca caattcttcc ccgatagcga tcagagtcag tttaatttag ttgttgaggc
      241 agcaccgggc agttcgcttg aacagacaag tttaatatgc gaaaaagctg aagctgtcat
      301 cagaaaaaac cctgaagtaa aaactatact tactactatc ggatccggaa atgatccggt
      361 tactaaagca actatcctgg taaaactggt agatacaaaa gacagaaaaa ggtctgacaa
      421 gaagctaatg tctgaaatca gaaaggaact aaagtatata cccggagcta aattcagtat
      481 gcgtgtccag ggcggaccgg gcggaaatga aaaaccgata acactcagca ttcgcggaga
      541 ggatatcact gaaatacaaa aaattgccga taaggtagaa gcaattgtta aaacaacacc
      601 cggtgcagta gatgttgata acagtcttga attatcaaaa ccggaaataa gaattaatat
      661 agacagagag aaagcttctg atcttgctgt aaatcctttc ttgattgctt ctacaattcg
      721 tgcgatggtt gacggagcgg ttgcaactca atttaaagaa ggtgatgagc agattgatgt
      781 cagggttaga ttaagaaaag ataatcgaag aaacataaat gatctttcat tgatcacagt
      841 taaaagcaat aaaaaaattg gcaaccagga ttttcttgtt ccaataacag atattgtaac
      901 aatcactaag gatgtcggtc cgtctaaaat aaacagatat gcaagacaaa aagaaattag
      961 agtggatgca aatcttgatg gagtgttttt aggtgtagcc ttaggtaaga tacttgaaca
     1021 aacctccaag ctcgaattaa aaccgggcta ttcaataaat gtaattggac agggagaaat
     1081 gcaggatgaa tcaatggcaa atatgctcat tactctgctg cttgcagttg tgttcgttta
     1141 tatagttctg gcagcacaat tcgatagttt tatacatccg ttctctatta tgctcgctct
     1201 gccgatgtca ataatcggag cggtgctggc tttattaata ttcggaagct ctctttccgt
     1261 tatgtctatg atcggtatta ttatgcttat gggacttgta accaaaaacg gaattcttct
     1321 tgtggatttc acaaacgttt tgcgcgaaag aggaatgtcg cggtttgatg cactggtaaa
     1381 agcaggacca acaagattgc gtccgatact tatgactaca tttgcaatga tattcggaat
     1441 ggttccggtt gcacttgcac ttggtgaagg tgctgaattc cgtgctccga tgggccaggc
     1501 agtaatcggc ggattgatta cctctacgct gttaacatta tttattgtgc ctgttgttta
     1561 ctctttactt gatgatctag gtacattcag cttttcagga ttctttaaaa aattattttc
     1621 cagacgtaaa aaacagaatt aggtaaaaag atgaaactta catttttagt ttatcatgat
     1681 attcttgatg atagagttac agaagcttta aagggacttg aaatagattt ttacacccag
     1741 tgggaagatg taaaaggtaa agggcataac acagatgcgc atctcggtaa tcgtcctttc
     1801 ccgggattta atttcgttag aatgattgca tttaccgaag aagatttgct tgaaaaactt
     1861 atcgccgaga taaatgaatt gaacaaaata gtagaaagag atgatgataa gattcgactt
     1921 tttcaggtac cgttggaacg aatagtttga cccttcagca aataatcaat ttagttggta
     1981 tttagttttt ctgtttaaat aaaagcctct cctaaaga
//
LOCUS       DASWBY010000387         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_579453, whole genome shotgun sequence.
ACCESSION   DASWBY010000387 DASWBY010000000
VERSION     DASWBY010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579453"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1987)
                     /gene="sprA"
                     /locus_tag="VF870_07030"
     CDS             complement(<1..>1987)
                     /gene="sprA"
                     /locus_tag="VF870_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HEX9251979.1"
                     /translation="ETIRLYTHGVDSMVHFRITEFNLVGSQWQKNLPDTIERTDTVLS
                     ISVVSLEENLNYTSPPGVFQERDRTRPDEEIFRNEQALSLVLSGLEEGDKREVVKYLF
                     RPLDVFNYKQMKLFVHGDENNSPGSISYSDTLAGNYQYSSEVYFRFGGDTNNYYEYRQ
                     PVRYNPAPGSNGWDEITIKFDDLTALKQLRGDDVTIPFRQSVDGAPPGHFYVIKGNPT
                     LTTVKFLSIGVVNLNSEFNPGPLSGEVWVNELRVIEADDSPGWAYSVSSSVKLADFAN
                     VSVNFSEKNPYFHRLADRFGSRVEQRNWSVSTDVNLLKLIPLNLPESNLRLNYSHTES
                     TGKPLYLPGSDVKVDKAVENQQILYENDTTGLVKSPELLKEETETYSVSDSWSASNIK
                     LKIPTKWWLIRDSFNAITMGFNYNNAYSRSPTVFSNTSWIWNANLNYGISLSPDYYFN
                     PIDIPIVGFLFSLLKDYSGTKIYFTPQNFSLALTAKRNESTNITRARNNSPSNEVSSR
                     DFTTSRGFNFTWKLTEGGFLNLTTSYSVNINSSLAYLLTNADSAGTSRPESEIWNDIF
                     NGAFFGEDNRYQQNFDIRTAPKLPSLFDINKYFTLTASYGVSYQWNYDFRQEIVGRSA
                     GFSSKSTIGLTMRWKSLFDPFFKEEVSKDKPPVGNLVG"
BASE COUNT          632 a          378 c          329 g          648 t
ORIGIN      
        1 tccaacaaga ttgccaacag gtggtttatc cttggaaact tcttccttaa aaaatggatc
       61 gaataatgat ttccatctca tcgtcaatcc gatagtagat ttgctgctaa atcccgcact
      121 tctgccaact atctcctgac ggaaatcata attccactga taactaacgc cataagatgc
      181 tgttagtgta aagtatttgt taatatcaaa aagtgatgga agttttggcg cagttctaat
      241 atcaaaattt tgctgataac gattatcttc gccaaaaaat gcaccattaa atatatcatt
      301 ccatatttcg ctttcggggc gcgatgtacc agctgaatca gcgtttgtta gtaagtaagc
      361 gagagatgaa tttatattaa cggaataact tgtggttaga tttaaaaatc caccttccgt
      421 taatttccat gtaaaattaa atccgcgtga agttgtaaaa tcccttgatg aaacttcatt
      481 agaaggtgaa ttatttctcg cacgtgtaat attagttgac tcatttcttt ttgctgtcaa
      541 tgctaatgaa aaattctgcg gagtaaaata aattttagtt ccgctgtaat cttttaacag
      601 actaaaaaga aaaccaacaa tagggatatc tatcggatta aagtaataat ccggacttaa
      661 agatatacca tagtttaaat tcgcattcca aatccagctt gtatttgaaa aaaccgttgg
      721 gctgcggcta taagcattat tataattaaa acccattgtg atagcgttaa aagaatctct
      781 tatcagccac cacttagtag gaatttttaa tttaatattt gatgctgacc atgaatctga
      841 aacagaataa gtttccgttt cttcctttag cagctctggt gattttacta atcctgtcgt
      901 atcattttca tagaggattt gctggttttc aacagcttta tcaaccttaa catcgctgcc
      961 cggtaaatac aatggcttgc cggtactttc tgtatgagaa taatttaatc gtaaattact
     1021 ttccggaaga tttaatggaa ttaattttaa tagatttaca tcggtggaca ctgaccaatt
     1081 acgctgttcg accctgcttc caaatctatc tgctagtcta tggaaatatg gatttttctc
     1141 gctaaagttt actgaaacgt tagcaaaatc cgcaagttta actgatgagg agacgctata
     1201 agcccatcca ggcgagtcat ctgcttcaat aactcttaat tcattaaccc aaacttcacc
     1261 agaaagcgga cctggattaa actctgaatt taaattcaca acaccaatag ataaaaactt
     1321 aacagttgtt aatgttggat taccttttat gacatagaaa tgacctggcg gagctccgtc
     1381 tacagattgc ctaaatggaa tagttacatc atcgccgcgc aattgtttta atgcggtaag
     1441 atcatcaaac ttaattgtta tttcgtccca accattcgat ccaggagctg gattatatct
     1501 taccggctgc cggtattcat aatagttatt tgtatcgccg ccaaacctaa aatatacctc
     1561 agaagagtat tgataatttc ctgcaagagt atcagaataa gaaatagaac caggactatt
     1621 attctcatca ccgtgaacaa aaagtttcat ctgtttataa ttaaaaacat caagtggacg
     1681 gaataaatat ttcactactt cacgcttatc tccttcttct aaaccactta aaacaagtga
     1741 aagagcttgt tcatttctaa atatttcttc gtctggtctt gttctatctc tttcctgaaa
     1801 aacgccggga gggcttgtgt aatttaaatt ttcttcaaga ctgacgacag aaattgaaag
     1861 tactgtatct gttctttcaa ttgtatcagg aaggttcttt tgccattggc ttccaacaag
     1921 attaaattct gtaattcgga agtgaaccat gctatcaaca ccgtgcgtat ataatcttat
     1981 agtttca
//
LOCUS       DASWBY010000388         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_653143, whole genome shotgun sequence.
ACCESSION   DASWBY010000388 DASWBY010000000
VERSION     DASWBY010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653143"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VF870_07035"
     CDS             <1..1189
                     /locus_tag="VF870_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX9251980.1"
                     /translation="NTGLKVVYGDTDSLFVMLKDIQTTEHAAGGYVIEPNPGIYENII
                     VLDFKSLYPSIIQTFKIDPYSLLMKDVDTIQTLNGYKFSSSLHILPDFIDELMKLRDV
                     AKKKKDKQLSQAIKILMNSFYGVMGSYGCRFYHPDLPRAITGSGHKLLLGSKDYLENT
                     GLKVVYGDTDSLFVMLKDISVDDGETQGKKIVKELNHFWKNKLKKEFNVESYLELEFE
                     KYYRKFIITPARGADIGAKKRYAGLVTKDGKETIEFVGMEFVRSDWTKLAKEFQVGLY
                     QKVFDGVEVEDWIREEIQKLKSGKYDDKLIYRKRLRKEVEDYTKNVPPHARAAKLLNE
                     PRDIIYYAITKRGPIPIELKHNDFDYDHYIEKQLKPIADSVLSLLGKNFDDIAGNDQL
                     SFF"
     gene            1248..1757
                     /locus_tag="VF870_07040"
     CDS             1248..1757
                     /locus_tag="VF870_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251981.1"
                     /translation="MKTKNILIVVFIFLLMIVNFGNEPTPPPYYQFVISGSLFCDTLV
                     DKSNYTIGVWGKVNLHSEDFLQINNTSLGTEYPIVLSDSLGNYNLLVNNYYFLDSIKL
                     GIIQPQLPTFFSESYSIDQSTGIPEERYYNGDGGSGCNSCTTEHTAATERIIKYQYQL
                     DSTKIFLCR"
     gene            1829..2722
                     /locus_tag="VF870_07045"
     CDS             1829..2722
                     /locus_tag="VF870_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9251982.1"
                     /translation="MKLKLSNQITAILLALFVTFLWSTSFVIIKLGLKEIPPLVFAGL
                     RYSIAFVVLLPFLFTSKNKQVVKNLTKKDWLNLTLLGILFYAITQGTQFIGLSLLPAV
                     TVSLWLNFTPLIVAVLAIFLIKEIPTVLQIFGVTLFITGILTYFYPVNLSSYQTTGLI
                     VMTIGVLANAVSAVLGRSVNKKSHLNPVVITVFSMGIGSFILLSVGITLQGLPNITTE
                     NIFYLLWLAVVNTALAFTIWNYTLRTLTAMESSIINGTMLIQIAVLAWIFLGEDISFK
                     EIIGMLIASSGAVLVQLKFKK"
     gene            complement(2838..>3270)
                     /locus_tag="VF870_07050"
     CDS             complement(2838..>3270)
                     /locus_tag="VF870_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX9251983.1"
                     /translation="NFKGVERFPIDESWKLNATFETYNPPKEIEVPNVLGQISKEKSP
                     GAVVFKRDGKKYKIDAVDEGGDRLFLIIADETSGEETYGGGRFMYVNKPDSTGTIFLD
                     FNKAYNPPCMFTKYATCPMPPLQNYLKLPIKAGEKVYGKGH"
BASE COUNT         1037 a          561 c          581 g         1091 t
ORIGIN      
        1 aaacacagga ctaaaagttg tttacggaga tactgattca ttatttgtaa tgctaaaaga
       61 tatccaaaca acagagcacg ccgcgggtgg atatgtgatt gaaccaaatc cgggaattta
      121 tgaaaacata attgtattgg attttaaaag tttgtatcca tcaatcattc aaacatttaa
      181 gatcgatccg tattctttat tgatgaaaga tgttgacaca atacaaacat taaatggtta
      241 taaattttca tcatcgcttc acatacttcc tgattttata gatgagttga tgaaattgcg
      301 tgatgttgca aaaaagaaaa aagacaagca gctttctcag gctatcaaaa ttctaatgaa
      361 cagtttttac ggtgtaatgg gttcttacgg ctgtagattt tatcatcccg atcttccacg
      421 agcaattacg ggaagcggcc ataagctttt acttggaagt aaagactatc ttgaaaacac
      481 aggactaaaa gttgtatacg gggatactga ttctttattt gtaatgctta aagacatttc
      541 tgttgatgat ggtgaaactc agggtaaaaa aattgttaaa gaactaaatc acttttggaa
      601 gaacaaactt aaaaaagagt ttaatgttga atcatactta gaattagagt ttgagaaata
      661 ttacaggaaa tttattatca ctcctgcacg cggcgctgat attggtgcaa agaaaagata
      721 tgccggactt gtaacaaagg atggaaaaga aactattgag tttgttggaa tggaattcgt
      781 gcgctctgat tggacaaaac ttgcaaagga atttcaggtc ggtttatacc aaaaagtttt
      841 tgatggtgtt gaagttgaag attggattcg tgaagaaata caaaaactta aatcaggcaa
      901 gtatgatgat aaacttattt atagaaaaag attacgcaaa gaagttgaag actacactaa
      961 aaatgtaccg ccgcatgcac gtgcagctaa attattaaat gagccccgtg atataattta
     1021 ttatgcaata acaaagcgcg gtccgattcc gatcgaacta aaacataacg attttgatta
     1081 tgatcattat atcgaaaaac aattaaaacc aattgcagat tctgtgctct cgcttctcgg
     1141 caaaaacttt gatgatattg caggcaatga tcaactgagt ttcttctagt gtagcccacc
     1201 ttaaaggtgg cctacactat agtacatttt aagaaagaaa atgccttatg aaaacaaaaa
     1261 atattttaat agttgttttt atctttttat taatgattgt aaactttggc aacgaaccga
     1321 ctcccccgcc atattatcaa tttgtaattt caggttctct tttttgtgat actcttgtgg
     1381 acaaatcgaa ttacacaatt ggcgtgtggg gtaaagtaaa tcttcatagt gaagactttt
     1441 tacaaatcaa taataccagt ttaggtactg aatatccgat tgttctaagt gactctttag
     1501 gtaactataa tcttcttgtt aataattatt atttcttgga ttcaataaaa ctgggaatta
     1561 tacaaccaca gcttccaaca tttttcagcg aatcatattc aattgatcaa tcaaccggca
     1621 tcccagaaga gagatattat aatggtgatg gtggttcggg ttgtaatagc tgcacaacag
     1681 aacatactgc agcaactgag cgtataatta aatatcaata tcaacttgat tctactaaaa
     1741 tattcctatg cagataaaca atattgttgt gatatttttt gctaagcttc aactggaaaa
     1801 tccctttgct ttttttattt tagtttagat gaaattaaaa ctctccaatc aaattaccgc
     1861 aatactactt gcgctgtttg taacctttct ctggtcaaca tcctttgtga taataaagct
     1921 tggattaaaa gaaattccac cgcttgtatt tgcaggatta agatactcga ttgcatttgt
     1981 tgtgcttctt ccctttttat ttacaagcaa gaataaacaa gttgtaaaga accttacaaa
     2041 aaaagattgg cttaacttaa ctctgcttgg aattctattt tatgcaatta cacaaggcac
     2101 acaatttatc ggactctctt tacttcccgc ggttactgtt agcttgtggt tgaactttac
     2161 tccgcttatt gttgcagtgc tggcaatttt tttaataaaa gaaattccaa ccgtgctaca
     2221 gatatttggt gtaacattat ttataaccgg catactcact tacttttacc cggtaaactt
     2281 aagcagttac cagacaaccg gattgattgt tatgacgatt ggagtgcttg ccaacgcagt
     2341 ttctgctgta cttggcagaa gtgttaataa aaaatcacat cttaatcctg ttgtaataac
     2401 agtatttagt atgggcattg gttcttttat tttgttaagt gtggggatta ctctgcaagg
     2461 attacccaac attacaactg aaaatatttt ttatcttcta tggcttgctg ttgtaaatac
     2521 tgctttagct tttacaatct ggaactacac actgcgcaca ttaactgcaa tggaatccag
     2581 cataataaac ggaacaatgc ttatacaaat tgcggtgctt gcatggatat ttctcgggga
     2641 agatatttcg ttcaaagaaa tcattggaat gctgattgct tcttcgggtg cggttttggt
     2701 acaacttaag tttaagaaat gatttttcct ttgcaatttt tgctaaaaac ctttgcgggc
     2761 tttgcgtgaa atgcgcttaa ggaatttctc gcaaagggcg cagagtaaaa catgcgaagt
     2821 tctctatgaa ttatttctta gtgccctttc ccataaactt tttcaccggc cttaattggt
     2881 aacttcaaat aattctgtaa tggcggcatt gggcacgttg catactttgt aaacatacaa
     2941 ggtggattgt atgctttatt aaaatctaaa aatattgttc ccgttgagtc cggtttgttt
     3001 acatacataa atctgccgcc gccgtaagtt tcttctccac ttgtttcgtc tgcgataatt
     3061 agaaacaacc tgtcgccacc ctcatcaaca gcatctattt tatatttttt gccatcacgt
     3121 ttaaacacaa ctgcgccggg ggatttttct ttacttattt gtccaagaac atttggcact
     3181 tcaatttctt taggaggatt atatgtttca aaggttgcat taagtttcca gctctcatcg
     3241 attggaaatc tttctactcc cttaaagttt
//
LOCUS       DASWBY010000389         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_729861, whole genome shotgun sequence.
ACCESSION   DASWBY010000389 DASWBY010000000
VERSION     DASWBY010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729861"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VF870_07055"
     CDS             <1..323
                     /locus_tag="VF870_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9251984.1"
                     /translation="EKGIFVFTIFFSAFIYTAIYLLVKKIKFDKSTFYLGLILGVPNV
                     LAIHFLLAALSLLPAIIVFPIQNIGVIVITAVAAYLFWKEQINLYGKIALAVGIAAII
                     LLKL"
     gene            complement(371..1873)
                     /locus_tag="VF870_07060"
     CDS             complement(371..1873)
                     /locus_tag="VF870_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9251985.1"
                     /translation="MSKPRLSFWQIWNMSFGFLGIQFGFALQNANVSRIFETLGASID
                     SIPILWIAAPVTGLIIQPIIGHWSDKTWNRLGRRRPFFLVGALLASAAIIIMPNSPAL
                     WVAAGMLWIMDASINISMEPFRAFVGDMLPSEQRTVGFSMQSFFIGIGAIVASALPYM
                     MTNWFGIANTAAAGEIPDSVKFSFYIGGAVFFIAVLYTVFSTKEYSPEELKKFSDDEK
                     KLSASGTADSNLISAASFNKNGLIWIFVGLLGSAILHFVLHENDYGLYVLFVGSIIFG
                     LLQLAAGFFTNQNKTTGGLVAILNDLYKMPKTMKQLAVVQFFSWFALFAMWIYTTPAV
                     THHIYGATDPSSELYNQGADWVGVLMAVYNGFAAVMAFFIMWVAKLTNRKTVHMISLI
                     IGGISFASFYFIKDPNLLLVSELGIGLAWASILAMPYAILAGSLPAEKMGVYMGIFNF
                     FIVIPQITAAAILGFFVKNIVGGEAIYALLLGGASMIIAGLMVMFVDDVD"
     gene            2028..>2576
                     /locus_tag="VF870_07065"
     CDS             2028..>2576
                     /locus_tag="VF870_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251986.1"
                     /translation="MNITEIINTVLYTPNAAFFYTPPIYGKSESYLFLKPKEIVIVKS
                     FRNLDEKLNRIDDLISDGLVGYSLMTYEAGYLFEKTLNKYLPKNESLIQFFFYDKKDV
                     QTIKSNKILFNSSEKFHVKNFKLNATKNEYEQSIRKIRYYIEEGDTYQVNYTVKGKFD
                     FNGSYSSLFASLVFNQSARYTAL"
BASE COUNT          908 a          452 c          456 g          760 t
ORIGIN      
        1 cagaaaaggg gatttttgtt ttcacaatct tcttttcagc ttttatttac actgcaattt
       61 atcttttagt taaaaagatt aagtttgata aaagcacatt ttatttagga ttgatccttg
      121 gagttccaaa cgtgctagcg atacattttc tgctggccgc acttagttta ttgccagcaa
      181 taattgtttt cccaatccag aatatagggg taattgttat aacagcagtt gcagcatatt
      241 tattctggaa agaacaaata aatctttacg gtaaaattgc attggcagtt ggtattgctg
      301 caataatttt gttgaagctg taaatttaat ttggaacgga ttggagttcg ttccctataa
      361 aatatttatt ttaatccaca tcatccacaa acattaccat tagcccagca attatcatcg
      421 aggcaccgcc aagaagtagt gcgtatattg cttctccgcc gacaatattt ttaacgaaga
      481 aaccaagtat tgccgcagca gtaatttgcg gaattacaat aaagaaatta aatattccca
      541 tatacacacc cattttttct gcaggaagtg agccggcaag aattgcataa ggcatagcaa
      601 gtatagaagc ccaggctaat ccaattccaa gttcggaaac taaaagcaga ttaggatctt
      661 tgataaagta gaaggatgca aatgaaattc cgccaatgat aagtgaaatc atatgaacag
      721 tttttctgtt tgtgagtttt gcaacccaca taatgaaaaa cgccataaca gcagcaaaac
      781 cattataaac agccatcaaa actccaaccc aatcggcacc ttgattgtat aattctgaag
      841 agggatcagt cgcaccataa atgtgatgag tcactgcagg agttgtgtaa atccacattg
      901 caaataatgc aaaccacgaa aagaattgaa cgactgcaag ttgcttcatt gtttttggca
      961 ttttgtaaag atcattcagg atagcaacta aaccaccagt agttttattt tgatttgtaa
     1021 aaaatccggc agccaattga agtaatccaa aaattatcga accaacaaat aaaacataaa
     1081 gtccataatc attttcgtga agaacaaagt gaagaattgc agagccgagc aatccgacaa
     1141 aaatccatat caatccgttt ttattaaatg atgcagcaga gattaaatta gaatcagcag
     1201 ttcctgatgc agataatttc ttttcgtcat cagaaaactt ttttagttct tccggtgagt
     1261 attcttttgt ggaaaaaact gtgtaaagaa cggcaataaa aaaaaccgct ccacctatat
     1321 aaaatgagaa tttaacagaa tcaggaattt ctcctgcagc agcagtgttg gcaattccaa
     1381 accagtttgt catcatataa ggcaatgcag atgcaacgat cgcaccaatt ccaataaaga
     1441 agctctgcat tgaaaatcca accgtacgct gttctgaagg aagcatatca ccaacaaaag
     1501 cacgaaaagg ttccatggag atattgatgg aagcatccat aatccagagc attccggctg
     1561 caacccaaag ggcgggggaa ttgggcataa taataattgc tgcagatgcg agcaaagctc
     1621 cgactaaaaa gaaaggtcgt cttcttccaa gtctgttcca ggttttatcg ctccaatgtc
     1681 caattattgg ttgaataatt aaaccggtaa ccggtgctgc aatccagaga ataggaattg
     1741 agtctatgct tgcgccgagg gtctcgaata ttctgcttac attagcattt tggagtgcga
     1801 atccgaattg aataccaaga aatccgaaac tcatattcca gatttgccaa aaggaaagac
     1861 ggggtttgct cataagtgtt ctcgagtatt attaaattaa cagtggttaa aaatagtaaa
     1921 tcggtattaa ttaagataat cgaaaaaaat cttttcactt cgtgtcttgg tgactttgtg
     1981 gctatgagtt ttgccactaa gtcacaaagg ttcactaaga aaaacaaatg aatatcactg
     2041 agatcataaa tacagtttta tatactccca acgctgcttt cttctacact ccgccaattt
     2101 atggaaagag tgaatcatat cttttcctaa aaccaaaaga aattgttata gtaaaatcgt
     2161 tcagaaatct ggatgaaaaa ttaaatcgaa tagatgatct tatttcagat ggtttggttg
     2221 gatattcttt gatgacttat gaagcaggct atttatttga gaaaacttta aataagtatt
     2281 taccaaaaaa tgaaagcttg attcaattct ttttttatga taaaaaagat gttcaaacaa
     2341 taaaatcaaa taaaatattg tttaattcat ctgaaaagtt tcacgttaag aacttcaaac
     2401 tgaatgcaac aaagaatgaa tatgaacaat caatcagaaa aatcagatat tatatagagg
     2461 aaggtgatac ttaccaggtt aactacacag ttaaaggcaa gtttgatttt aatggttcat
     2521 actcaagttt gtttgctagc ttagtattta atcaatctgc acgatataca gctctt
//
LOCUS       DASWBY010000390         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_241545, whole genome shotgun sequence.
ACCESSION   DASWBY010000390 DASWBY010000000
VERSION     DASWBY010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241545"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VF870_07070"
     CDS             <1..997
                     /locus_tag="VF870_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX9251987.1"
                     /translation="VECSKCHPKEAKNSKEFIRFKGLKFANCSGCHTDVHKGAFGADC
                     KSCHTVNGFQIINQQNFDHSKTKFPLMGKHIVVKCNDCHKGADKKKPQFAKCLNCHAD
                     YHQGEFTLNGAVKDCIVCHSENGFNISSFSIEEHNKSKFQLTGAHLAVPCKNCHQQGN
                     EWKFKNVGLDCLDCHKNIHGTELSTKYLPENKCTFCHQTENWSIIKFDHNSTEFVLLG
                     KHKNATCGKCHSTKADSEQKVFKFSSLNNNCEQCHKDIHYGQFKVDKTTTCENCHTFN
                     DWKPEKFDHNKTKFSLDNAHKKVPCIRCHPSTTENGNKFFVYKITEFKCATCHTK"
     gene            973..>1904
                     /locus_tag="VF870_07075"
     CDS             973..>1904
                     /locus_tag="VF870_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251988.1"
                     /translation="MRYLSYQIIFFLFTISIFAQSPHGDSFNLDCSECHSADSWKIDL
                     TKISFDHSKTNFALVGQHINLDCKSCHQSLVFSKMDKDCFSCHKDIHQGTVGLDCANC
                     HTPATWVVKDIIGLHQKGRFPLLGAHQTADCAQCHSGYPELKFDPLNIDCYSCHKQNY
                     ESAQIPNHIAANFSTECQDCHNISALSWNTTNIDHSFFPLVGGHALPNCYSCHQRGGN
                     FSGLSSECYSCHQQTYEATQNPNHISAGLPTTCDVCHSIYGWAPAQFNHDLTAFPLTG
                     KHINTDCSNCHASGYTGTPTDCYSCHQQDYQGTT"
BASE COUNT          651 a          325 c          335 g          593 t
ORIGIN      
        1 agttgaatgt tctaaatgtc atccaaaaga agcgaaaaac agtaaagagt ttataagatt
       61 taaaggcctg aagtttgcaa attgttcagg ctgccacact gatgttcaca aaggtgcttt
      121 tggtgctgat tgtaagtctt gtcatactgt aaatgggttt caaattatta accagcaaaa
      181 ttttgatcat agtaaaacga agtttccgtt gatgggaaaa cacattgtag ttaaatgtaa
      241 tgattgccat aagggtgctg ataaaaagaa accgcaattt gcaaaatgcc tcaattgcca
      301 tgctgattat catcaaggtg aatttacttt aaatggagca gtaaaagatt gtatagtttg
      361 tcactctgaa aatggattta atatttcaag tttttcaatt gaagaacaca acaaaagtaa
      421 attccagtta acaggtgcac atttagctgt cccgtgcaaa aattgtcatc agcaagggaa
      481 tgaatggaag ttcaaaaatg tcgggttaga ctgcctagat tgtcataaaa acattcacgg
      541 tacagaactg tccacgaaat atttaccgga aaataagtgt acattctgtc atcaaactga
      601 aaattggagt attattaaat ttgatcataa tagcacagaa tttgtattat taggcaaaca
      661 taaaaatgca acttgcggaa agtgtcatag tacaaaagca gattcagaac agaaagtttt
      721 taagttctct tctctaaata ataattgcga gcagtgtcat aaagatattc attatggtca
      781 gtttaaggtt gataaaacta caacctgcga aaattgtcat acatttaatg actggaagcc
      841 tgaaaaattt gatcataata aaacaaaatt ttctttggat aatgcacata aaaaagttcc
      901 ttgtatcagg tgtcatccat caaccacaga aaacggaaat aaattttttg tttacaagat
      961 aacagaattt aaatgcgcta cttgtcatac caaataatat tttttctttt tacgataagc
     1021 atttttgcgc aatcacctca tggtgatagt tttaatcttg attgttcaga atgtcacagt
     1081 gcagatagct ggaaaataga tcttaccaaa atttctttcg atcattccaa aactaatttt
     1141 gcactagttg gtcagcatat aaatttagat tgtaaatcct gtcatcaatc tttagttttt
     1201 tcaaaaatgg ataaagattg cttttcatgt cataaagata ttcatcaagg tacagtagga
     1261 ttggattgtg caaattgcca tacaccagca acatgggttg taaaagatat tattggactt
     1321 catcagaagg gaagatttcc tctgcttgga gcacatcaaa cagccgattg tgctcaatgc
     1381 cattcaggtt atccggaact aaaatttgac ccattaaata ttgattgcta ctcatgtcac
     1441 aaacagaatt acgaatcagc ccaaattcct aatcatatcg cggcaaattt ttctactgaa
     1501 tgccaggatt gccataatat tagtgcactt tcatggaaca ctactaatat agaccatagt
     1561 ttctttccac ttgtgggcgg acatgctttg cctaattgtt attcctgtca tcagcggggt
     1621 ggaaattttt cgggactttc atcagagtgt tattcatgtc atcagcagac atatgaagca
     1681 acacaaaatc caaatcatat atctgcagga ttgccgacaa cttgcgatgt ttgtcattct
     1741 atttatggct gggctcccgc tcaattcaat cacgatttaa cagcttttcc attgacgggt
     1801 aaacacataa atacagattg ttctaattgt catgcttcag gttacactgg aacaccgaca
     1861 gattgttatt cgtgccatca acaagattat caaggcacaa caga
//
LOCUS       DASWBY010000391         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_351862, whole genome shotgun sequence.
ACCESSION   DASWBY010000391 DASWBY010000000
VERSION     DASWBY010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351862"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="VF870_07080"
     CDS             <1..1490
                     /locus_tag="VF870_07080"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX9251989.1"
                     /translation="HPVVAIPELMRILIDDENHSWDEAWNITSKTFAYTNHTVVPEAL
                     EEWSEQIFGELLPRHLQIIYEINRRFLEDVKVNYSKDQNVLDKLSIISGGKDKRVRMA
                     NLAIVGSFAVNGVAALHTNILKKRIFPDFNKIYPKKFVNVTNGITPRRWIVTANPLLT
                     QLITEKIGQEWIKDLTQIKKIEKFVNNKDFIENWQMIKLNNKKILIDYIEKEHLIKVN
                     PDSIFDVQIKRFHEYKRQLLNVFHIITLYNRIKKDPKIKIVPRTIIFGGKAAPAYQAA
                     KMVIKLINSVADVVNNDPDVGDKLKVVFLKNYSVTLAEKIIPASDLSEQISTAGLEAS
                     GTGNMKFALNGALTIGTMDGANVEIREEVGDDNIFIFGLLADEVVKLKSNGYNPKTYY
                     EKNAELKHVVDMIATNFFSPNEFGIFDDMIRGLMEVDYYLLFADYQVYVDAQDKVAQL
                     YKNEEEWTKKSIYNVARVGKFSSDRSVKEYADKIWKVKPVKVNHS"
BASE COUNT          575 a          241 c          300 g          460 t
ORIGIN      
        1 ctcatcccgt tgtagctata ccggagttaa tgcgaatact tattgatgac gaaaatcata
       61 gctgggatga agcttggaac atcacttcta aaactttcgc ctacacaaat cacactgtgg
      121 tacccgaagc tttagaggaa tggtctgaac aaatttttgg agaattgctt ccgcggcatc
      181 ttcaaataat ttatgagatc aacagaaggt ttttagagga tgtaaaagta aattattcta
      241 aagaccagaa tgttttggat aaactttcta ttatttccgg aggtaaagat aagcgcgtta
      301 gaatggctaa tctggctatt gtaggaagct ttgctgttaa tggtgtagct gcactgcata
      361 caaatatttt aaagaaaaga atctttcctg attttaataa aatttatccc aaaaaatttg
      421 taaacgttac aaacggaatt actccaagac gatggatagt aacagccaat ccgcttttaa
      481 ctcagttaat tactgaaaaa atcggacagg aatggataaa agatcttacg caaataaaga
      541 agatagaaaa gtttgtaaac aataaagact ttattgaaaa ttggcaaatg ataaagttaa
      601 ataacaaaaa gattcttatt gattacattg aaaaagaaca tctcattaaa gtcaatcctg
      661 attcaatatt tgatgtacag ataaaaagat tccacgaata caaacgacaa ctgctgaatg
      721 tttttcacat cataacgctt tataacagaa tcaaaaaaga tcctaaaatt aaaatagtgc
      781 cgcgtaccat tatctttggc ggtaaagccg ctccagcata tcaggcagca aaaatggtca
      841 tcaaattaat taattctgtt gcagatgttg ttaataatga tccggatgta ggcgataaac
      901 taaaagttgt gtttcttaaa aactattcag ttactcttgc agaaaaaatt attcctgctt
      961 ctgacttatc agaacaaatt tctactgcag gattagaggc atcgggaaca ggcaatatga
     1021 agtttgcact taacggtgca ttaacaatcg gtacaatgga tggcgcaaac gttgagatca
     1081 gagaagaagt gggcgatgat aacatattta tcttcggcct gcttgccgat gaagttgtaa
     1141 aactaaaatc gaacggatac aatcctaaaa catattatga aaaaaatgct gagcttaagc
     1201 acgttgttga tatgattgca acaaatttct ttagtccaaa tgaatttgga atttttgatg
     1261 atatgattcg cggattgatg gaggtggatt attatctctt gtttgcagat tatcaggttt
     1321 atgtagatgc gcaggataaa gttgcacagc tgtataagaa tgaagaagaa tggacgaaaa
     1381 aatctattta taatgttgca cgggttggaa agttttcaag cgacagatca gtaaaagaat
     1441 acgcagataa aatctggaaa gttaaaccgg taaaagttaa tcactcataa aaattggaag
     1501 ttaatggatt acgaattaaa tccccgcaca cgtattggac atgttcattt aaccgtagct
     1561 aatcttgata aagcat
//
LOCUS       DASWBY010000392         3863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_201817, whole genome shotgun sequence.
ACCESSION   DASWBY010000392 DASWBY010000000
VERSION     DASWBY010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201817"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF870_07085"
     CDS             <1..213
                     /locus_tag="VF870_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251990.1"
                     /translation="KAEGLESYDVYEVKGKAVSFQELYTFTSMEAYEAFDDLNDERIN
                     ILVDKISGMSVDKTTKYNTLVQLADE"
     gene            306..1181
                     /locus_tag="VF870_07090"
     CDS             306..1181
                     /locus_tag="VF870_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251991.1"
                     /translation="MNKKLHLIYVGSFFVVGVAVLVLLAINGYQYYSTPFEERFFNPS
                     HAALKPSGNWGHGFGIIGTLMMIFGVAIYMIRKRSRKLFTFGYLKHWLEFHIFLCTVG
                     PILVLYHTAFKFGGIVSVSFWSMVLVVFSGVVGRFIYVQIPRTIQGKEIDINDLASIR
                     EELTDKMKLEMLFDVRLMKELDVLASPERYKSLNMLDTLLLYFKDFFRIRSFQSKLKK
                     NLVVAGFSKFKRKEIKNKAQAEIILSRRLGMLRTMQNLFRYWHVAHLPFAIAMFVIMI
                     IHVIVTLAFGYKWIF"
     gene            1193..1792
                     /locus_tag="VF870_07095"
     CDS             1193..1792
                     /locus_tag="VF870_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251992.1"
                     /translation="MKFKPIYFYGIIAVVVVIILIVVSQTSNNEKVNVDITNKQMPMD
                     DVHKNLNSGGMQNPTGSNVTEEVKHKIEVMKKDVESNPNDTLKIREYADFLAAAHKPD
                     EALLYYQKIIDKDKKRKDIYFAITYVHYTKGNLAKAEEVTRTMLQIFPGDPMAIYNLG
                     AIEATKGNKEKAREIWTKLIKDNPNDETSELAKNSLNKL"
     gene            1873..3204
                     /locus_tag="VF870_07100"
     CDS             1873..3204
                     /locus_tag="VF870_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9251993.1"
                     /translation="MEQLLENIFAYVLVFLLLGGIFYYYLMKNKIKSVQTVSKTAKAK
                     QYGFHEPVSLHPVVDLDICIGSGACIKACPEQDILGINNGQAQTINASRCVGHGACYH
                     ACPVEAISLCIGTEKRGVELPHISKEFETNIPGIFVAGELGGMGLIKNAVEQGKQAVE
                     YYLKKSYSKSKAKYDLIIVGAGPAGISASLTAAKNNLKFLTLEQDTLGGTVFSFPRAK
                     IVMTSPMDLPLWGKVKLVETTKSELLDLWNNVLKKNNISINEYEKVTEIVKQDNMFIV
                     KTDKEHYTSNGIILAIGRRGSPRKLGVPGEDRENVFYRLLEPELIHDLDILVVGGGDS
                     AVESAMLLADEKNRVTLSYRGENFSRLKPKNLERVNDYIKKKKIKTLFNSNVKEIFKN
                     EVCLTFNDRPDEMKLKVDLTYVFAGGILPTSFLEEIGIKITKKFGDALLKH"
     gene            3212..>3863
                     /locus_tag="VF870_07105"
     CDS             3212..>3863
                     /locus_tag="VF870_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX9251994.1"
                     /translation="MKYIFIVLFFSFKLIAQISPGDLTKVHADLEGISNCTKCHEIGE
                     KVLSSKCLDCHTEIKSLINANKGYHAYSEVKKRECASCHNEHHGRNFRIVNFTETDFD
                     HSKTGFKLTGAHYNKDCKDCHQSKYITENKLKKRSKTFLGLDQRCSSCHEDYHQKTLG
                     DDCTSCHNTEKFKPAANFDHNKSEFKLAGGHLKVECSKCHPKEAKNSKEFIRFKGLK"
BASE COUNT         1375 a          567 c          712 g         1209 t
ORIGIN      
        1 aaagcagaag gcttggaatc atacgatgtt tacgaggtaa aaggtaaagc cgttagcttt
       61 caggaactat acactttcac ttcaatggaa gcttatgaag cttttgatga cttgaatgat
      121 gaacgcataa atatccttgt agataaaata agtggtatgt cagtcgataa aaccacaaaa
      181 tacaacacac ttgttcaact tgcagatgaa taaatttagc gggtctctaa acagacccct
      241 ttatttttta tcaatgatac ttttcttaat ttaaatcact tactaattat atccatttaa
      301 ctcggatgaa taaaaaacta catctaattt atgttggaag tttttttgtt gttggcgtag
      361 ctgttttagt tctattagct attaatggct atcaatacta ctcaactcca tttgaagaga
      421 gatttttcaa tccatcacac gctgcattaa aaccaagcgg caactggggc catggttttg
      481 gaattatcgg cacactaatg atgatttttg gggtggcaat ttatatgatt cgtaagcgat
      541 ccagaaaatt gtttacattt ggatatttaa aacactggct ggaatttcat atttttttgt
      601 gtactgttgg tccaatttta gtcttgtatc atactgcatt taaatttggt ggaatcgttt
      661 ctgtaagttt ctggagtatg gttcttgtgg tgtttagcgg ggtagttggc agatttattt
      721 atgtccagat tccaagaaca attcagggta aagaaattga tattaatgat ttagcatcta
      781 tacgtgaaga acttacagat aagatgaaat tagaaatgct ctttgatgtc agactaatga
      841 aagaacttga tgtacttgct tcaccggaaa gatataaatc tttaaatatg ctggatactt
      901 tattgctgta ttttaaagat tttttccgta tcagatcatt tcaatctaag cttaagaaaa
      961 atttggtagt tgcaggtttt tcaaaattca aaagaaaaga aataaaaaat aaagcacagg
     1021 cagaaattat attatcaaga agacttggta tgcttagaac aatgcagaat cttttccgtt
     1081 actggcatgt tgcacatctt ccatttgcca ttgccatgtt cgtaataatg ataattcacg
     1141 ttattgtaac tcttgcattt ggatacaaat ggatttttta ggaggtataa aaatgaaatt
     1201 caaaccaatt tatttttatg gaattatagc tgttgttgtt gtaataattc taatcgtagt
     1261 ttctcaaaca agcaacaacg aaaaggtaaa tgttgatata acgaataaac aaatgcctat
     1321 ggatgatgtt cataaaaatc ttaatagtgg aggaatgcag aatccaaccg gctcaaatgt
     1381 tactgaagag gttaagcata aaattgaagt aatgaaaaag gatgttgaat caaatcctaa
     1441 tgacacatta aagattagag aatatgcaga ttttttagct gccgctcata agcctgatga
     1501 agcattatta tattatcaaa aaattattga taaagataaa aaacgtaaag acatttactt
     1561 tgcaataact tatgttcact atacaaaagg caatcttgca aaagctgaag aagttactcg
     1621 cactatgttg caaatatttc ctggggaccc aatggccata tacaatttgg gtgcaatcga
     1681 agccacaaaa ggcaataaag aaaaagctcg tgagatttgg acgaagctga taaaagataa
     1741 tccgaatgat gagacatctg aacttgctaa gaattcatta aataagctat agtatatatt
     1801 gttgcttgcg gcaaggaaca atttttgaga ttatatagat acttaattcg ataactaatt
     1861 aaatctaact ctttggaaca attactcgaa aatatttttg catacgtact tgtattttta
     1921 cttttaggtg gaatatttta ctactacctg atgaaaaata aaatcaagtc cgtccagact
     1981 gttagtaaaa ctgctaaggc aaaacagtat gggttccacg aacctgtttc tttgcatccg
     2041 gttgttgatc ttgatatttg tattggaagt ggtgcatgta ttaaagcctg tccagagcaa
     2101 gacatattag gaataaataa tggtcaagct caaactataa atgcatcaag atgcgttggg
     2161 catggggcct gctatcatgc ctgcccggta gaagcaattt ctctctgtat tggaacagaa
     2221 aaacggggtg tggagttacc gcatatttca aaggagtttg aaacgaatat tccaggaata
     2281 tttgtcgccg gtgaacttgg cgggatgggt ttgataaaaa atgctgttga acaaggtaaa
     2341 caagctgttg aatattattt aaagaaatct tattcaaaat ctaaagcaaa gtacgatttg
     2401 ataattgttg gcgctggtcc agcaggcata tcggcatcac taacagctgc taaaaataat
     2461 cttaagttct taacattaga acaggatact ttaggaggaa ctgtttttag ttttccccga
     2521 gccaagattg tgatgacatc acccatggat ttaccgcttt ggggaaaagt taaattagtt
     2581 gagacaacaa aatcagaatt acttgacctt tggaataatg ttctgaaaaa aaataatatc
     2641 tctattaatg aatatgaaaa agtaactgag attgtaaaac aagataatat gttcattgtt
     2701 aaaactgata aagaacatta tacaagcaat ggaattatac ttgcaattgg cagacgaggt
     2761 tctccaagaa aacttggtgt gccgggtgaa gatagagaaa atgtttttta ccgattactt
     2821 gaaccagagt taattcatga tcttgatatt cttgtcgtcg gcggaggtga ttcggccgtt
     2881 gaatcggcaa tgctgcttgc tgatgaaaaa aatcgtgtta cactttctta cagaggtgag
     2941 aacttttcca ggttaaaacc aaaaaatctt gagcgagtta atgattatat caaaaagaaa
     3001 aaaataaaaa ctttatttaa ttctaatgta aaagaaatat ttaaaaatga agtttgttta
     3061 acttttaacg atagaccaga tgaaatgaaa ttaaaagttg atcttaccta tgtttttgcc
     3121 ggtggaatat tgccaacgag ttttcttgaa gaaattggaa tcaaaataac aaagaaattc
     3181 ggagatgcac tcttaaagca ttaattgact agtgaaatac atttttatcg tactattttt
     3241 ttcttttaaa ctaattgctc aaatctcacc aggcgatctt acaaaagtcc atgcagattt
     3301 agagggtatc agtaattgta caaagtgtca cgaaattggt gaaaaagttc taagttccaa
     3361 atgtttggat tgccacacag aaattaaatc cttaataaat gctaacaaag gttatcacgc
     3421 atactcagaa gtaaagaaaa gggaatgtgc atcttgccat aacgagcatc atggaagaaa
     3481 ctttagaatt gtaaacttta ctgaaactga ttttgatcat agcaaaaccg gattcaaact
     3541 tacaggcgca cactataata aagattgtaa agactgtcat cagtctaaat atataactga
     3601 aaataagtta aaaaagagaa gcaaaacttt tttaggatta gatcaaagat gctcttcctg
     3661 tcatgaagat tatcatcaaa aaactttagg tgatgattgt accagctgcc ataataccga
     3721 aaaatttaaa cctgccgcaa attttgatca caacaaatca gaattcaaat tggcaggcgg
     3781 acatttaaaa gttgaatgtt ctaaatgtca tccaaaagaa gcgaaaaaca gtaaagagtt
     3841 tataagattt aaaggcctga agt
//
LOCUS       DASWBY010000393         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_730040, whole genome shotgun sequence.
ACCESSION   DASWBY010000393 DASWBY010000000
VERSION     DASWBY010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730040"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..850
                     /locus_tag="VF870_07110"
     CDS             200..850
                     /locus_tag="VF870_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9251995.1"
                     /translation="MKKRTFITLISLFVFLLFIQQKEQWTSVFLVTDQPDVLMKGNHG
                     VTLTVDLSFGREDVDEWITKLEKPYPLLFLDADWIERSPLIIKKIKQKNIPVGLLGQE
                     GKQYEEAPDLLKKEIERFQSEFGVVPLWYRTKDHTFPKPLKKQLWDSKINMVSSSVNW
                     TTGNPPTIQNGDIVSVALHQKTRVEIKELTSFQKAHPFVSIEETLFGYKTQTKTFP"
     gene            complement(877..1602)
                     /locus_tag="VF870_07115"
     CDS             complement(877..1602)
                     /locus_tag="VF870_07115"
                     /inference="COORDINATES: protein motif:HMM:NF025455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KinB-signaling pathway activation protein"
                     /protein_id="HEX9251996.1"
                     /translation="MIQSDEELEKNGGFFVVTIRNWFIFFFKALVIGGLVTGVLGLII
                     RWQDVFAPFVASGEYAGFAVSFLWFVFLGMTMSVLSQMGFFAYLTVHQFGVNIFKSLT
                     LWNWVQLLIIALVIFDLIRFRFMPIANTQSQLWLYLGLLAILLTTGLVVAYFKAKWTK
                     KHAFISALFFMIVITTLEWLPVLMVDEKDVDRYVTILLFPLLAVNAYQLLKLPKYNAK
                     SDVDRLKLEERRKARTQATKVKA"
     gene            1703..2290
                     /gene="gerD"
                     /locus_tag="VF870_07120"
     CDS             1703..2290
                     /gene="gerD"
                     /locus_tag="VF870_07120"
                     /inference="COORDINATES: protein motif:HMM:NF040801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination lipoprotein GerD"
                     /protein_id="HEX9251997.1"
                     /translation="MRKWIILIGIVLLAGCGGAQPSSNPSYEETKKMITDTMQTEEGK
                     KVMRQMLSDPEFKELLILEQEDVKSSIENTLLSKDAEKFWKDTFKDPTFSETFAKSMK
                     EQQQDIMTDLMKDASFQKELETFFGQPDMQKQLETILQSATMRKEIEEIVQETVDSPL
                     LKTKWQELIKAAGAAEEEKSGESGGGQKKAEEKKP"
     gene            complement(2333..>3316)
                     /locus_tag="VF870_07125"
     CDS             complement(2333..>3316)
                     /locus_tag="VF870_07125"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX9251998.1"
                     /translation="TNGIKEVSIKEEKNHVSVKLAIAKINTAEQMQLQMKVVDTLKGA
                     GVRTVGIRFEELPAEILAQFRGTATASEAQDLLSPLNKVEFISIASGKGGVGKSTVSV
                     NLAVALARLGKKVGLIDADIYGFSVPDMMGIQQSPKIEGTRIIPVERFGVKVISMGFF
                     VEDNAPVVWRGPMLGKVLDQFFKDVEWGELDYLLLDLPPGTGDVALDIHQMIPSSKEI
                     VVTTPHPTAAFVAARAGAMALQTDHEVLGVIENMAWFQSKTSNEKEYVFGKGGGPKLA
                     DELRTELLGQIPLGQPDWNEEDFAPSVYSETHSTGKIYEEIALKVINKVKK"
BASE COUNT         1123 a          655 c          557 g          981 t
ORIGIN      
        1 cgtcaccgtt tcaacagcaa ctcttatatc ttaccaaaac ctaattgtca tgtcaacaaa
       61 aagttttgac ttaacatatt tgtttgtcac ttgctttagc gacttatgta tcttaccatc
      121 cttcaaacat gaatgtcaat acaatatcat aaatattaaa gagcaaaaat agactagtta
      181 acgaagggtt gtgaatccaa tgaaaaaacg tacgtttatc accttaataa gtttgttcgt
      241 tttcttacta tttattcaac aaaaagagca atggacatcc gtgttccttg taactgatca
      301 acctgacgtc ttgatgaaag gcaatcacgg cgtgacgtta actgtagatt tgtcttttgg
      361 ccgagaagac gtggatgaat ggattactaa attagagaaa ccttatcctc ttcttttttt
      421 agatgcagac tggattgaac gttctccttt aattattaaa aaaattaaac aaaaaaacat
      481 tccagttgga ttgctcggtc aagaaggcaa gcaatatgaa gaagctccag atttgctaaa
      541 aaaggaaatc gaacgttttc agtctgagtt tggtgtagtt ccactttggt atcgaacaaa
      601 agatcacaca tttccaaaac cacttaagaa acaactatgg gattccaaaa tcaatatggt
      661 gagctcttcc gttaactgga caacaggtaa tcctcctact attcaaaacg gagatatcgt
      721 ttcagttgca ttacaccaga aaactcgagt agagattaaa gagctaactt ctttccaaaa
      781 agctcatccg tttgtttcta tagaagaaac attgtttggc tataaaacac aaacgaaaac
      841 ttttccataa aaaaagcatc ccactggatg ctttttttat gcttttactt tagtagcttg
      901 agtacgtgct tttcgacgct cttccaactt taatcgatcc acatcagact ttgcattgta
      961 ttttggtaat tttaataatt gatatgcatt taccgctagt aatgggaaca ataagattgt
     1021 tacataacga tctacgtctt tttcatctac catcaatacg ggtagccatt ccaatgttgt
     1081 aataacaatc ataaaaaata gcgcagatat gaaagcatgt ttcttcgtcc atttggcttt
     1141 gaaataagct acgactaatc ctgtcgttag aagaatagcc agaagtccta aatataacca
     1201 caattgtgat tgcgtattag caatcggcat aaaacgaaat ctaattaaat caaaaataac
     1261 taatgcaata attaatagtt gcacccagtt ccacaatgtt agggatttaa aaatattaac
     1321 tccaaattga tgaactgtta aatatgcgaa gaatcccatt tgactcagaa cactcatcgt
     1381 catacctaaa aatacaaacc ataaaaaact tactgcaaat ccagcgtact ctccacttgc
     1441 aacaaaaggt gcaaatacat cttgccatcg aataattaat ccaagcacac cggtaacgag
     1501 cccgccaatt acaagtgcct tgaagaaaaa tataaaccaa tttcgtatcg tcacaacaaa
     1561 aaatcctccg tttttctcaa gttcttcatc tgattgtatc aacgaataag caaaaaatct
     1621 aatttactat tcgatatcta catattctca agaaatttgt gaacaataaa tgaaaaggtc
     1681 agtggaagaa aagaggttaa gtatgcgaaa atggataatt cttataggaa tcgtcttatt
     1741 agcaggttgt ggtggagcac aaccaagcag caatccttct tatgaagaaa caaaaaaaat
     1801 gattacggat accatgcaaa ctgaagaagg aaaaaaagta atgcgtcaaa tgctttcaga
     1861 tcctgaattt aaagagcttt taattttgga acaagaagat gtgaaaagtt cgattgagaa
     1921 tactctactg tcaaaggatg cggaaaaatt ttggaaagat acgtttaaag atccaacttt
     1981 ttctgaaacc tttgcaaaaa gcatgaaaga acaacaacaa gatatcatga ctgacttaat
     2041 gaaagatgct tcatttcaaa aagagttaga aacattcttt ggacaacctg atatgcagaa
     2101 acaattggaa accatcttgc aatcagctac aatgcgaaaa gaaatcgaag aaattgtgca
     2161 agaaaccgtt gatagcccgc ttctcaaaac taaatggcaa gaattgatta aagctgctgg
     2221 tgccgctgaa gaagagaaat ccggtgaatc tggtggtggc cagaaaaaag cagaagagaa
     2281 aaaaccgtaa aaacacaaaa ccccgcttgc acatgcaaac ggggttttac acttatttct
     2341 ttactttatt tataactttt aacgcgattt cttcatatat tttacccgtt gaatgtgttt
     2401 ctgaatacac agaaggagca aaatcttctt cattccaatc aggttgacct agtgggattt
     2461 gtccaagaag ttctgtgcga agctcatctg cgagcttcgg accgcctccc ttaccaaaaa
     2521 catactcttt ctcattagat gttttcgatt ggaaccacgc catattttct attacaccta
     2581 gtacttcatg atctgtttga agcgccatag cacctgcacg cgcagctaca aaagccgctg
     2641 ttggatgcgg agtggttaca acgatttctt ttgatgaagg gatcatttga tggatatcaa
     2701 gtgccacgtc tcctgttcct ggtggtaaat caagcaataa ataatctaat tctccccact
     2761 caacatcttt gaagaattga tctaagactt taccaagcat tggaccacgc catacaactg
     2821 gtgcgttatc ttctacgaaa aagcccatcg aaatgacttt aacaccaaag cgttcaactg
     2881 gaattatgcg tgtaccttct atttttggac tttgttgaat gcccatcata tcaggtacgc
     2941 tgaagccata aatatccgca tcgattaatc caactttttt acctagacgc gctaatgcca
     3001 ctgctaagtt aacggaaact gtagatttcc ctacgcctcc cttaccagaa gcaatggaga
     3061 taaattcgac tttatttaat ggtgacaata aatcttgtgc ttcagaagca gttgccgttc
     3121 ctcggaattg tgctaatatt tctgcaggca attcttcaaa gcgaatgccg acagtacgaa
     3181 cgcccgctcc tttcaacgta tcaactacct tcatttgcaa ttgcatttgt tcagcagtat
     3241 ttattttagc gatcgcaagc tttacgctaa catgattttt ttcttcttta attgatactt
     3301 cttttatacc gtttgt
//
LOCUS       DASWBY010000394         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_351979, whole genome shotgun sequence.
ACCESSION   DASWBY010000394 DASWBY010000000
VERSION     DASWBY010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351979"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VF870_07130"
     CDS             complement(<1..543)
                     /locus_tag="VF870_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9251999.1"
                     /translation="MTPTIKQIAQKANVSIATVSRALNNDVRVTEVTREKILRISNDL
                     NYKPNLVARNFVKKTSYAIGLILPDISDEFFTDIIRGVDEITYKNGYYTMVASSHKHR
                     TLEDSIATFSQTGLVGGVILLMSAMTDDLKNILDQAKIPVILIGAGKQERDFDIVTID
                     NYQGAFNATEYLIKKKKFKRI"
     gene            complement(612..1709)
                     /locus_tag="VF870_07135"
     CDS             complement(612..1709)
                     /locus_tag="VF870_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252000.1"
                     /translation="MRRFLLASVLLFSVALYAQPVIDFETVGNNWVWNPFDLGSTGMF
                     DIVANPSPGGLNTSDSCAMFMIAADGNPWGGVYCTEFPDMTITADNCMLKILVYKDGI
                     SPVNIKLEPPNQDHFVSNTLINQWEELTFDYSAAIGTTAITLTLIPDQKPDYPRTYAS
                     VNYIDYIRFTPQVVPVELTSFTGAFVGNDVQLKWATATELNNRGFEIQRSISGSAFAT
                     IAFVEGHGTTTEQKQYSFIDRNVTAGVNHAYRLKQIDFNGAYEYSDVVNVGTPLPVEF
                     ALEQNYPNPFNPTTNIAYAVPVKSNITLDVYNLIGQKVVTLFEGEVEAGKHSAQFNAS
                     SMSSGIYLFKLTAVGENGSQFSSSKKMTLLK"
     gene            complement(1892..2971)
                     /locus_tag="VF870_07140"
     CDS             complement(1892..2971)
                     /locus_tag="VF870_07140"
                     /inference="COORDINATES: protein motif:HMM:NF021554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HEX9252001.1"
                     /translation="MIETMNLAGKILSVVLLFMLPDTSGQDSTNSKLGYISYSVPSRS
                     HFSGEIAYLFKYHSVYQFKESKFDITALPTYLNSERVMNDGTSWTEYSDINYFRLSYM
                     IGLMAATNTVAYIYQRKVWYTEETTVFHSLEFINDWNKYQQMDKFGHFSDAYFTSDLT
                     GKIYRWSGMSGNTSVWLGAITGWAWMLEIELSDAFMSEWGFSWGDMLANTAGSAFYIL
                     QQFNYEALGGIHPKFSWHKSEAWKENRYNKDPQALIEDYEGMTFWLTVNPHHYFPDSW
                     KKNYSEWLAPLGLAFGVSAKDIGIYPWGGYKEYFVGLDIDLRKLPIWDDWGFFKFVKS
                     ELNFLRLPLPTIRFSPHGTWFGFYF"
BASE COUNT         1014 a          564 c          511 g          983 t
ORIGIN      
        1 aattctttta aattttttct ttttaatcaa atactctgtt gcattgaatg cgccctgata
       61 attatcaatt gtaacgatat caaaatcacg ttcctgttta ccagcaccaa ttagtatcac
      121 tggaatttta gcttgatcca aaatattttt taaatcatct gtcatcgcag acattaataa
      181 aataacacca cctactaatc ctgtttgcga gaatgttgca atagaatctt caagggtacg
      241 atgtttatga gaacttgcaa ccattgtgta ataaccgttt ttataagtta tctcatcaac
      301 accgcggata atatctgtaa aaaactcatc tgagatatcg ggcaggatta aaccaatggc
      361 gtatgaagtt ttttttacaa aatttcttgc aactaaattt ggtttgtagt taaggtcgtt
      421 agatatccgt agaatttttt ctctggttac ttctgtaaca cgaacatcat tattaagagc
      481 tctggaaacg gtggcaattg atacattagc cttttgcgcg atctgtttta ttgtgggagt
      541 catagtatta tttaaatcag aatggaagca gaatatttct attctgcttc caagaatgtt
      601 tagtaaaagt attacttcaa tagcgtcatt tttttgcttg atgagaactg cgatccattt
      661 tcaccaactg cggttaattt aaataaataa attcctgaag acattgatga tgcattgaat
      721 tgtgctgaat gttttccagc ttcaacttca ccttcaaaca atgtaacaac cttctgtccg
      781 ataagattgt aaacatcgag tgtaatatta gattttacag gaactgcata agcaatattg
      841 gttgttgggt taaaaggatt tggataattc tgttcaagag caaattcgac tggtaatgga
      901 gtacctacat taacaacgtc tgaatattcg tatgctccgt taaagtcgat ttgttttaat
      961 ctgtaagcgt ggttaacacc agctgttaca ttacgatcta taaaagaata ttgtttttgt
     1021 tctgtggttg ttccatgacc ttcaacaaaa gcgatagttg caaaagcaga gccacttatg
     1081 cttctctgga tttcgaatcc tctattgtta agttctgtag cagtagccca ttttaactga
     1141 acatcatttc caacaaaagc accggtgaat gatgttaatt caacaggaac aacttgagga
     1201 gtgaatcgga tataatcaat ataatttaca cttgcataag ttcttggata gtcgggcttt
     1261 tgatcgggaa ttaaagtcaa tgtaattgca gttgtaccaa tagctgcaga ataatcaaat
     1321 gtcaattctt cccactgatt aattaacgta tttgagacga agtgatcttg attaggcggt
     1381 tcaagtttta tattaacagg actgatacca tccttgtata ctaaaatttt tagcatacaa
     1441 ttgtcagcag ttatagtcat atcaggaaac tcagtacaat aaacacctcc ccaaggatta
     1501 ccatcagcag cgatcataaa catagcacaa ctatctgatg tatttaatcc accaggactt
     1561 gggtttgcaa ctatatcaaa cataccagtt gatccaagat caaaaggatt ccacacccag
     1621 ttattaccaa cagtctcgaa atcaataacc ggctgagcgt acagagcaac agagaacagc
     1681 aacactgaag ctaatagaaa tcgtctcata gcaccctcct ttaaaaggat ttgtaaataa
     1741 gttaattaga aatagaatgt aaacgtttac attgtggttt taaactaatt caatattctt
     1801 aaattgtcaa gaggttagag ggaaatatta attatttaaa gtttaaaatt gattctactt
     1861 tgagtttaaa gaaattaatt aagatgatat cctaaaaata aaatccaaac caggttccat
     1921 gaggagaaaa gcgaatggtg ggtaatggca aacgaaggaa attcaattca ctttttacaa
     1981 atttaaagaa accccaatca tcccaaatgg gaagctttct cagatcaata tcaagcccaa
     2041 caaagtattc tttatatccg ccccagggat aaataccaat gtcttttgca cttactccaa
     2101 atgccaggcc taatggtgct aaccattcag aataattttt tttccaacta tcgggaaaat
     2161 agtggtgtgg attaaccgtt aaccagaaag tcataccttc gtaatcttcg attaaagctt
     2221 gcggatcttt attatatcgg ttctctttcc aggcttctga tttatgccag ctaaactttg
     2281 gatgaattcc acctaacgct tcatagttaa attgctgcag aatatagaag gcagaacctg
     2341 cggtattggc aagcatatct ccccagctaa acccccattc tgacataaag gcatcagata
     2401 attcaatttc cagcatccac gcccatccgg ttatggctcc cagccacaca gaagtatttc
     2461 cagacattcc ggaccatcga tatatttttc ccgtaagatc actcgtaaaa tatgcgtctg
     2521 aaaaatgacc gaatttatcc atttgctgat atttgttcca atcgttgata aactctaaag
     2581 aatgaaaaac agttgtttct tcagtatacc ataccttgcg ctgataaata tatgctaccg
     2641 tgttggtagc cgccattaaa ccaatcatat aactaagccg aaaataatta atatctgagt
     2701 attcagtcca acttgttcca tcattcataa ctctttcact atttagataa gttggtaatg
     2761 ctgtaatatc aaatttagat tctttgaatt gatagactga atgatatttg aagagataag
     2821 caatttctcc tgagaaatga gatcttgaag gaactgagta gcttatgtag cctaacttgg
     2881 agttagttga atcctgccca gaagtatcgg gaagcataaa gagtaataca acagataata
     2941 tttttccagc gagattcata gtctcaatca tcattatcaa aaaatatctt gattaaaaac
     3001 agaaatcaat tttccagata ctcttttctt acagctcttt taaataatcc gggccgccga
     3061 gtgaatttat tt
//
LOCUS       DASWBY010000395         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_241750, whole genome shotgun sequence.
ACCESSION   DASWBY010000395 DASWBY010000000
VERSION     DASWBY010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241750"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2792)
                     /locus_tag="VF870_07145"
     CDS             complement(<1..>2792)
                     /locus_tag="VF870_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEX9252002.1"
                     /translation="WDLVFTNDDIYELNNGDKLPFVISVTCYTAHFDNQEIFGEIFNS
                     VPGKGSIAFWGSSGVTFWPTTANINQDLFAAIFNNKKYVIGKAIDAAKSNQNYGTMTA
                     LLTLLGDPALKLAIPTKPDFVIKSSNLSIYPTNPLVNDTVSVKLAINNLGLSFPGDTV
                     TLKLYERIISDTTLVDSISLTNFGESDTTYFNWVPKESGLITIIATINDDRRVPEDDF
                     SDNNASNSFSIFDFGEPSIVKPANGYFTSSGNIDFLLSDIGFYFDRDFNYLIQLNDSP
                     QFDGVPLLAQSPVLKANDAVVKWNPGTLNPGEYFWRAIVYDNVDTNSSVTRIFTISDK
                     SGSGFLAQKKQLDLFNNTNVNYSEQYGSLVLSTELNPPYPSPKFLLDSIYYSLPTDQT
                     KPSTFTTDGTYFYFGNLPDFNNGGKSKIYKIGTGLNGTVAGTNYGAIPNLEVYIYSHL
                     MFHDGFLYTCTGPTDNLLKIDTNTGDTSRVAIPDNLLLTVASSTQIGGVFLYSDGQFV
                     YNLGVGTSLYPTKFVLRKFDPANNWAKVGEDIICSGTTLKRVSSFIISENYLIVYENY
                     TLIKLRRYSLPNGIFEEEWWYAPKIKDYYAIAYDHVNNFVYFNTFRPGVTPYIPAFFK
                     YEGTFIDANGQITSPDIGPASKWHNLEFNIDQTNSNGTYKGYLLGKNKSSGNWVTLDS
                     LIDRNISIENIDTKQYNFIKLRFDLEDLSSGSGEPMKFNSLQVNYEYLPEISIIPKNL
                     TFTPDSVLQGISLDMTLNVNNIGYVPVDSLKLEFSDNVTDSIFYTTYISVPNDTLSSS
                     VKSIITDKLLYTAPVSPVDVKVVATLPVEEYYTFNNLTNGTFYVVRDSTNPRFNITFD
                     GSEIINGDVISSEPEVVITLEDNSPLPLDSTYFTIVHTHNNVPKVLTVPGPDITYNYT
                     PYPNSRAVITWTPKLL"
BASE COUNT          969 a          465 c          457 g          901 t
ORIGIN      
        1 cagaagtttt ggggtccagg ttattacagc acgtgagttt ggataaggtg tatagttata
       61 agtaatatcc ggtcctggta cagttaaaac tttaggaaca ttattatgag tgtgtacaat
      121 tgtaaaatag gtactgtcta atggtaaagg actgttatct tcaagagtaa ttacaacttc
      181 cggttctgaa gaaattacat caccattaat tatttcgctt ccatcaaaag taatattgaa
      241 acgaggattt gtggaatcac gaacaacata aaaggttccg tttgtcaaat tattaaaggt
      301 gtaatattct tcaacgggca atgttgcaac aactttaaca tcaactggtg atactggcgc
      361 tgtgtaaagt agtttatccg taatgattga tttgacagat gatgatagag tatcatttgg
      421 aacactaata taagtcgtat aaaaaattga atccgttacg ttatcagaaa actctaattt
      481 taaactatcc accggaacat atcctatatt atttacattc agagtcatgt ccaaagagat
      541 gccctgcaaa actgagtcgg gagtaaatgt taaattttta ggtataatag aaatctcggg
      601 cagatactca taattcactt gcagtgaatt aaatttcatt ggctcgcctg aaccggaaga
      661 taaatcttct aaatcaaatc taagtttaat aaaattatat tgtttagtat cgatattttc
      721 gatgctaata tttctgtcaa taagcgagtc taatgttacc cagtttccac tacttttatt
      781 tttaccaaga agataacctt tataggtacc attagaattc gtttgatcaa tattaaattc
      841 aagattatgc cactttgaag caggaccaat atccggtgaa gttatttgac catttgcatc
      901 gataaatgta ccctcatatt tgaaaaatgc gggaatatat ggagttacac caggtctgaa
      961 tgtattaaaa tacacaaaat tattaacgtg atcataagct atagcataat agtccttaat
     1021 tttaggtgca taccaccatt cttcttcaaa aataccatta ggtaggctgt atcgtcgaag
     1081 tttaattaaa gtatagtttt cataaactat taaataattt tcagagatga taaaacttga
     1141 aactctcttc aaagttgtac cgctgcaaat tatatcttcc ccaacctttg cccaattatt
     1201 tgcaggatca aattttctta gaacaaattt agtggggtaa agtgatgtac ccacacccag
     1261 attataaaca aactgcccat ctgagtataa aaagacaccg ccaatttggg ttgaagaagc
     1321 aactgtcagc aataaattat ctggaattgc tactctactg gtatcgccag tatttgtatc
     1381 tatttttaga agattatctg ttggaccagt acaagtatag agaaatccgt catggaacat
     1441 aagatggcta tatatataaa cttctagatt agggattgct ccatagtttg tgccggcaac
     1501 tgttccattt aaacctgttc caattttgta aatcttagat ttaccaccat tattaaaatc
     1561 tggtaagttt ccaaaataaa aataagttcc atctgttgta aaagtagatg gttttgtttg
     1621 atccgttggc agactataat aaatggaatc caataaaaat tttggacttg gatatggcgg
     1681 atttaattca gtacttagaa caagactacc atactgctct gaataattaa cattagtatt
     1741 attgaaaaga tctaattgtt tcttctgggc aagaaatcct gagccacttt tatcagagat
     1801 tgtgaaaatt cttgtaacac ttgaatttgt atcaacatta tcatagacaa tagcacgcca
     1861 aaaatattct ccgggattta atgtacccgg attccatttt acaactgcat catttgcttt
     1921 taaaaccgga gattgagcaa gtaagggcac accatcaaac tgcggagaat catttaattg
     1981 aattaaataa ttaaaatctc tgtcaaaata aaatcctata tcagataaaa gaaaatctat
     2041 attgcccgaa gaagtaaaat aaccattcgc cggtttgacg atacttggtt cgccaaaatc
     2101 gaaaatggaa aaggaattag aagcgttatt atcagaaaaa tcatcctcag gtaccctccg
     2161 atcatcatta atggttgcta taatagttat caaccctgat tctttaggaa cccaattaaa
     2221 atatgttgta tcgctttcac caaaattcgt tagggaaatt gaatcaacta aagttgtatc
     2281 agaaattatt ctttcataaa gttttaaggt aaccgtatca cccgggaatg ataggcctaa
     2341 attattgata gctaatttta cagaaacagt atcgttaact aacggattag ttggataaat
     2401 tgataagttt gatgatttaa ttacaaaatc tggtttggta ggtatcgcca atttaagtgc
     2461 aggatcacca agcagcgtta ataaggctgt cattgtacca taattttgat ttgatttagc
     2521 agcatcaatc gctttaccaa taacatattt tttattatta aaaatcgcgg caaataaatc
     2581 ctgattaata tttgcggtcg ttggccagaa agtaaccccg gaacttcccc agaatgcaat
     2641 actgccttta ccaggaactg aattaaaaat ttctccaaat atctcttgat tatcaaaatg
     2701 tgcagtataa caagttacac taattacaaa aggcaattta tctccgttat tcaattcata
     2761 aatatcatca ttagtaaaga ccaaatccca tt
//
LOCUS       DASWBY010000396         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_277526, whole genome shotgun sequence.
ACCESSION   DASWBY010000396 DASWBY010000000
VERSION     DASWBY010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277526"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1712
                     /locus_tag="VF870_07150"
     CDS             <1..>1712
                     /locus_tag="VF870_07150"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEX9252003.1"
                     /translation="LIISIYCVAQQDESWKLYDDSQVARVDITIDPAALDWIYQNVQS
                     DSEFVATFHFQNAYINETVEIIGFRLRGNTSREAAKKSFKVSFNTFVQGREFYGVDKL
                     NLNGEHNDPSIIRSKLCFDHYQSIGIKASRANHVEVYINGQYYGLYISVEHIDDEFLN
                     KNFGDDTGNLWKCLYPADLTYLGSDPNLYKNLNNNGRQAYELTTNEAQGDFSKLVRLI
                     TLLNNTPSGSLADSLESVIEVPGVLKYFAMNILVGEWDDYWSLMNNYYLYHDPTNDIF
                     TLIPYDYDNTYGIDWFNINWANANPYNFPKVASGYRPLAERLMQNAQYRNLYTHFLEF
                     FRNNVYMLSNWENRIDSLKQMITTSALNDSFRTLDYGFDANDFFNSYSATGYNNQHVK
                     YGLKQFVNLRNSSLPAQLSYLPAEPIAYKIDYEPKNPSANDSIHVYASVFDNNGLNEV
                     SIYFQETGSSSTEIYQMNFSPVSNTKKVEEADRWVGVIPPLGAGKTGKFFVYVKDTQN
                     EFQFYPRKKAIEIKTQQIISNDIVINEFMADNAATITDPSGEYDDWIELYNPTSDPIV
                     LTGR"
BASE COUNT          588 a          289 c          291 g          544 t
ORIGIN      
        1 ctcattatct caatctattg cgttgctcaa caggatgaaa gctggaaact ttatgatgac
       61 tctcaagttg ctagagtaga tattacaatt gatcctgccg ctcttgattg gatctaccaa
      121 aatgtgcaaa gcgattcaga atttgtagca acatttcatt ttcaaaacgc ttacataaat
      181 gaaactgtag aaattattgg attcagatta cgcggaaaca cttcccgcga agcagcaaaa
      241 aaatccttta aagtctcatt taataccttt gttcaaggaa gagaatttta tggggttgat
      301 aaattaaatt taaatggcga gcataatgat ccatcaatta tccgaagcaa attatgcttt
      361 gatcattacc agtcaattgg aataaaagca agccgggcaa atcatgttga agtttatatt
      421 aatggacagt attacggact gtacatatct gttgaacata ttgatgatga atttctaaat
      481 aaaaattttg gtgatgacac aggtaatctc tggaaatgcc tttaccctgc tgatttaact
      541 tatcttggca gcgatccgaa tctttataaa aacttaaaca ataacggaag acaagcttat
      601 gaattaacaa caaatgaagc tcaaggtgat ttttctaaac ttgtaaggtt aataactcta
      661 ttaaataata ctccatccgg ttcgcttgct gattcattgg agtcagtaat agaggttccc
      721 ggagttttaa aatattttgc gatgaatatt ctggttggcg agtgggatga ttactggtcg
      781 cttatgaaca attactattt atatcacgat ccaacaaatg atatttttac tttaatcccg
      841 tatgattatg ataacacgta cggtatcgat tggtttaata ttaattgggc aaatgcaaat
      901 ccttataact tccctaaagt tgccagcgga tatagacctc ttgcagaaag attaatgcaa
      961 aacgcacagt acagaaatct ttacacacac tttctggaat tttttagaaa caacgtttac
     1021 atgctttcaa attgggaaaa tcgaattgac agccttaagc aaatgatcac aacctctgcg
     1081 ctgaatgatt ccttccgtac ccttgattat ggatttgatg cgaatgattt ttttaattct
     1141 tattcagcga cgggatataa taatcaacac gttaaatatg gattaaaaca atttgtaaat
     1201 cttagaaata gttctttacc tgctcagctt tcatatttgc ctgctgaacc aatcgcttat
     1261 aaaattgatt acgaacccaa aaatccttca gccaatgatt caattcatgt ttatgcatca
     1321 gtgtttgata ataacggttt aaacgaagta tcaatctatt ttcaggaaac cggttcatct
     1381 tcaacagaaa tttaccaaat gaatttttca cctgtatcaa atacaaaaaa agttgaagaa
     1441 gcagacagat gggttggtgt tattccgcca cttggggctg gtaaaacagg aaagtttttt
     1501 gtctatgtta aggacacaca gaatgaattt caattctatc caagaaaaaa agcaattgaa
     1561 attaaaacac agcaaattat ttctaatgat attgttataa atgaatttat ggctgataac
     1621 gcagcaacaa taactgatcc ttctggagaa tacgatgatt ggattgaact ttataatcca
     1681 acatcagatc cgattgtttt aaccggaaga ta
//
LOCUS       DASWBY010000397         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_730109, whole genome shotgun sequence.
ACCESSION   DASWBY010000397 DASWBY010000000
VERSION     DASWBY010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730109"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VF870_07155"
     CDS             complement(<1..634)
                     /locus_tag="VF870_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX9252004.1"
                     /translation="MAKKNSIRRIGILTGGGDCPGLNAVIRGVTKPAEDYGMSVYGII
                     DGFEGLVEGKAKELNNSDVSGILSRGGTILGSSNKGDPFHWPVEVNGKIEILDKSESA
                     LKNYKSWGLDALIAIGGDGTMHICNKLSKMGMNIVGVPKTIDNDLEATDVTFGHDSAV
                     YVVSMALDRLHTTASSHHRVIVVEVMGRYAGWIALNGGLSGGADIILIPEI"
     gene            complement(654..1574)
                     /gene="rnhC"
                     /locus_tag="VF870_07160"
     CDS             complement(654..1574)
                     /gene="rnhC"
                     /locus_tag="VF870_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEX9252005.1"
                     /translation="MQDPFQQKALKQIDLLAQKLINAGFSVSQPDKKLYNYEIIVNIN
                     KEQAKLLVYFGKKGIKNVIQANPESKIFAELNRIVFGETLFESELKTEIAYKEYIGTD
                     ESGKGDYFGPLVIAAVYIDEKSRPELEKVGVKDSKLITDNNIKIIELKIKKIIGNNFE
                     IVQINPEKYNKLYEAFNNLNKIMGWAHSKAIENLLQRIECSNVISDKFGNEKLIKDEL
                     KKKNIDLNLYQTPKAERYTAVAAASILARAKVVDWFNIKSRELGFQIPKGGGPQVNIS
                     ARRVLNQFDDNYLMKMIKFHFKNSQSIFKK"
     gene            1654..1983
                     /locus_tag="VF870_07165"
     CDS             1654..1983
                     /locus_tag="VF870_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252006.1"
                     /translation="MNTLRLNKYLLILSLIVVVSANNLLSKTVIQDNKAITEFTSILK
                     QKVLLNNDQEAKVLTIMNELQKNISSKPENKSDYVKTAQTKVESLLDSKQKMKYDIIK
                     NDLWKNI"
     gene            complement(2035..>2350)
                     /locus_tag="VF870_07170"
     CDS             complement(2035..>2350)
                     /locus_tag="VF870_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX9252007.1"
                     /translation="NRNFLDVGTGTASFYADEFNGKKTANGEVYNMNDLTAAHPSYPF
                     NTLVKVTNLSNNKSVEVRINDRMPDFKGRVIDLSYAAAKKIEMINAGIQEVKLEVIKW
                     GN"
BASE COUNT          755 a          443 c          307 g          845 t
ORIGIN      
        1 gaatttccgg aattaaaata atatccgcac caccgctaag acctccattt aatgcaatcc
       61 agcctgcgta tcgacccatt acttctacaa caattactct atggtgtgat gatgcagttg
      121 tatgaagtct atctaatgcc atagaaacaa catacactgc tgaatcatga ccgaatgtaa
      181 catctgtagc ttcaagatcg ttatcaatcg tttttggaac gcctacaatg ttcatcccca
      241 tcttagataa tttattgcat atgtgcatcg taccatcgcc gcctatagcg ataagtgcat
      301 cgagtcccca agatttataa ttttttaaag ctgattcaga tttatcaaga atttctattt
      361 taccatttac ttctaccggc caatggaaag ggtcgccttt atttgaagag cctaaaatag
      421 ttccgcctct tgataaaatt ccggagacat ctgaattatt taattctttt gcttttcctt
      481 caactaaacc ttcaaagcca tcaataattc cgtaaacaga cattccataa tcttcagccg
      541 gttttgttac acctcttatt accgcgttta atcccggaca gtcacctccg cccgtcaaaa
      601 taccaattct tcttatacta ttcttctttg ccatttttac tttataatcc tgattatttc
      661 ttaaaaatac tttgtgaatt tttgaaatga aatttaatca ttttcattaa ataattatca
      721 tcaaattgat tcaaaacacg tctggcagaa atattaactt gaggtcctcc gcccttggga
      781 atctgaaatc ctaactctct gcttttaata ttaaaccaat caacaacttt tgccctagct
      841 aatatcgatg ctgctgcaac tgctgtatat ctttctgctt ttggagtttg atatagattt
      901 aaatcaatat tttttttctt caattcatcc ttaataagtt tttcatttcc aaacttgtca
      961 ctaattacat ttgaacattc aattctttgt aaaagatttt cgatagcctt cgaatgcgcc
     1021 cagcccatta ttttatttag attattaaat gcttcataaa gtttgttata cttttcagga
     1081 tttatttgaa caatttcaaa attattcccg attatctttt taattttcaa ttcaataatc
     1141 ttgatgttat tatcagtaat tagtttgcta tcctttactc caactttttc taattcgggt
     1201 cttgactttt catcgatata aacggcagca attaccaacg gtccaaaata atcacccttt
     1261 cctgattcat ctgtaccgat atattcttta taagcaattt cggttttaag ttcagattca
     1321 aatagagttt caccaaatac aattcgattc aattcagcaa atattttcga ttccgggttt
     1381 gcctggataa cattcttaat gcctttttta ccaaaataga ctaataattt tgcctgctct
     1441 ttattgatgt tcactattat ttcataattg tacagttttt tatccggttg agaaacagag
     1501 aacccagcat ttatcagttt ttgggccaaa aggtcaattt gttttaaagc tttttgctgg
     1561 aatggatctt gcatacaagt aattgattta agtttgtatt taataagtaa taaaattagt
     1621 tttcatagta acgagattta aaaggagaaa ataatgaata ctttaagatt aaataaatat
     1681 ttattgatat tatcattaat agtagttgtt agtgctaata atcttttatc aaaaactgta
     1741 atacaggata ataaagcaat aactgaattt acttcgatac ttaaacaaaa agtgctgttg
     1801 aataatgatc aggaagcaaa agtacttact ataatgaacg agttacaaaa aaatatttca
     1861 tcaaagccgg aaaataaatc tgattatgtt aaaacagctc aaacaaaagt tgaaagcttg
     1921 ctcgatagca aacaaaaaat gaaatatgat ataatcaaaa atgatctatg gaaaaatatc
     1981 tagcaaacta ttaaacattt tgacccggtt taacgaccgg gttttttttg agatctaatt
     2041 accccactta ataacttcca atttcacttc ctgaataccc gcattaatca tttcgatttt
     2101 ctttgcagct gcataagata agtcgataac tcttccttta aaatccggca tccgatcatt
     2161 aattcttact tcaacacttt tattatttga aagatttgtg acttttacca aagtattaaa
     2221 tgggtatgat ggatgagctg ccgtcaaatc attcatatta taaacttcac cattcgcagt
     2281 cttctttccg ttaaattcat cagcataaaa cgacgcagtt cctgtcccaa catccaggaa
     2341 atttctgttt
//
LOCUS       DASWBY010000398         4732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_241899, whole genome shotgun sequence.
ACCESSION   DASWBY010000398 DASWBY010000000
VERSION     DASWBY010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241899"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VF870_07175"
     CDS             complement(<1..764)
                     /locus_tag="VF870_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX9252008.1"
                     /translation="MKKLLLASIFIFSGIFLSCSREEPFVAPPPPPEVINAKINEIYS
                     RGTVGNEDWIEIYNPSTSAMDISGYKIYDSGGESGSKPKKEFPAGTVIPAGGFFVIVV
                     DDTTESGFGLSSNGETVWLENTSGIIDSITFPALGVDTSYARNPDGSSVWVKLSPPTK
                     GESNSSGGGETQALVMNEFFSRGADPDFDWIEIYNPNSVQVELTGYKIYDGGGNLGTK
                     PKMEFPVGAVVPANGFYVIVVDIQDPAGFGLGSGGD"
     gene            complement(781..2061)
                     /locus_tag="VF870_07180"
     CDS             complement(781..2061)
                     /locus_tag="VF870_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9252009.1"
                     /translation="MNYKKHFVLIITISLLSILGVNELYAQDDKIPGGNQEVPLGVKE
                     RNGKLLFSSDNGDFQWWFDSRIQVDGAIYNENLNEMSNGIILRRATFALKAVLWKDWQ
                     AEFDMDFGEDVSVDNQTDWRDMWIKYTFPKFNLSLQVGNFKEPFGLERLGSSRMLTFM
                     ERSTVSGITLGRRMGGSARYWTNNWQVTAALMGHELGSRVDKGQQDEGWSTTLRLTAA
                     PINNFGENLHLGIAGSYKIPDVTTELRPNTIEVSARTETYVFDPKLLHTGDINNVNYF
                     NRYGFEFMYINGPFYFQSEFIGSSIYRWYGNPTVNLQGGYAMAAWVITGETRYYYVDE
                     GEVGPIEKPKYSWGALEVAARYSVTNLNDFDTDIHGGQSNQLMLGINYYPNMNIKLQF
                     NYSYVNLDEFATRKGNLYGNDDHSFIQMRVQASL"
     gene            complement(2085..3581)
                     /locus_tag="VF870_07185"
     CDS             complement(2085..3581)
                     /locus_tag="VF870_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX9252010.1"
                     /translation="MDISVWFFISSGLFLGWSLGANHAVNVFGTAVVSKMVKFRTAAL
                     ISGIFVILGATISGEGTTKTLTDLGSINAIAGSFSVALAVGISITLMTRAKLPVSTSQ
                     AVIGGIIGWNIFTGSPTDYNSLTKILFSWIASPVIAAIFGFLIFKLIKKTVLKWKIHL
                     LRLDNYTRIGLIIVGGLASYSLGANNIANVMGMFASAPLFKDLNIANIIVISSVQQLF
                     LLGGIAIAIGIFTYGNNVMETVGTDLYKISPITGFVVVLAEFIVLWLFTSQSLETFLL
                     KNNLPSFPLVPLSTTQAFIGAVIGVGLAKDPLSINFKVLGKIGIGWVVAPLTAGLITF
                     ISLFFIQNVFEQKIIHPVPYQITTSVIKKLNDEGIATEKLNDLLNKRFTNNKTFLNEL
                     KTRYDYNSKHIYSIYKYALIDSFKIDSLLINELPENFQLSITQKSKLKELHGRSFNHK
                     IDFEEAVYSNLPEWKLNGDKLNDKAIEEKKIIIEDLFRVTNKTKTNNY"
     gene            complement(3584..4222)
                     /locus_tag="VF870_07190"
     CDS             complement(3584..4222)
                     /locus_tag="VF870_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX9252011.1"
                     /translation="MFLKKTRKLETQIDEYLDLVIKGGLIFKLGIKCYLDHQIEAFEG
                     HLKDLRKIEETADDLRRNIEIKLYTRTLIPESRGDVLGLMESCDKALNITAETLLEFS
                     VEIPNLLPELKNDYLDIAESSISCLENTVSGIRAYFKNVDAVRDYVTKVQFYKKETNK
                     IAERIKRAVFRTDNDLSFKIHTRYFTFHIERIAEQSEDVCDRLSIAIIKRFE"
     gene            complement(4303..>4732)
                     /locus_tag="VF870_07195"
     CDS             complement(4303..>4732)
                     /locus_tag="VF870_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX9252012.1"
                     /translation="VTDLPSISVNAFFDHLGIGDYTQFWVLYLLLTGVMVFSALIFQS
                     KTMRAMIFIPIAIGVANRFGYDIISLALPVAFLIEHVYVLPFNSKPAALLYETDHYSL
                     TDTFKYGFTMMVISWLFIIIAGETWFRYLGITPNGVFGIF"
BASE COUNT         1535 a          916 c          702 g         1579 t
ORIGIN      
        1 tcatctccac cacttcctaa accaaatccg gctggatctt gtatatcaac tactataaca
       61 taaaatccat ttgcaggaac aacagcacca acaggaaatt ccattttggg ttttgtacca
      121 aggtttccgc ctccgtcata tattttatat cctgtcaatt caacctgcac agaatttgga
      181 ttataaattt ctatccaatc aaaatcagga tcagcacctc ttgagaaaaa ttcattcata
      241 actagagctt gagtttcccc accgcctgaa gagtttgatt cacctttagt aggtggagat
      301 aatttaaccc aaacggaaga accgtcagga tttcttgcgt aggaagtatc aacgccgagt
      361 gctggaaatg taatgctatc aatgatgcca ctcgtatttt ctaaccacac tgtttctcca
      421 ttacttgata agccaaagcc actttcagtt gtatcatcaa ccacaattac aaagaatcca
      481 ccagccggaa taactgttcc agcaggaaat tcctttttgg gtttgctgcc gctttctcca
      541 ccactatcat aaattttata accgctgatg tccatggctg atgtagaagg attatagatc
      601 tcaatccaat cttcgtttcc gactgtacct cgtgagtaaa tttcatttat ctttgcatta
      661 atcacttccg gtggcggcgg tggtgcaaca aatggttcct ctcttgaaca ggagagaaaa
      721 atacctgaaa atataaaaat agacgctaat aataattttt tcattttata ttcctttcat
      781 ttataatgat gcctgaacac gcatttgaat aaatgaatgg tcatcattgc catacaggtt
      841 tcctttacgt gttgcaaact catcaaggtt aacatacgaa taattaaatt gtaattttat
      901 attcatatta ggataataat ttattccaag cattagctga tttgattgtc caccatgaat
      961 gtcagtatca aaatcgttta aatttgtaac gctgtaccta gctgcaactt caagtgcacc
     1021 ccaggaatat ttaggttttt caattggtcc aacttcgcct tcgtcaacat aatagtaacg
     1081 ggtttcacct gtaataaccc acgcagccat agcatatccg ccttgcaaat ttacagttgg
     1141 atttccatac catctataga tggaggatcc gataaactca gattgaaaat aaaatggtcc
     1201 gtttatatac ataaattcaa atccatagcg attgaaatag tttacattat taatatcacc
     1261 cgtatgtaat aatttagggt caaatacata agtttctgtt cttgcactta cttcgatggt
     1321 atttggcctt aactctgtgg ttacatcagg aattttatat gatcccgcaa tacctaagtg
     1381 aagattctct ccaaaattat ttattggagc agctgttaaa cgtaaggtag ttgaccagcc
     1441 ttcatcctgc tgacccttat ctactctgga tcctaattcg tgacccatta atgctgcggt
     1501 aacctgccag ttatttgtcc agtatcttgc agaaccaccc atacgtcttc ctaaagtaat
     1561 tcctgaaaca gttgatcttt ccatgaaagt aagcattcta gaactgccta atctctccaa
     1621 accaaatggt tctttaaagt tacctacctg aagtgacaga ttgaacttag ggaaagtata
     1681 tttaatccac atgtctctcc aatctgtctg gttgtctaca ctaacgtctt ccccaaaatc
     1741 catatcaaat tctgcttgcc agtctttcca gagaactgct tttaacgcaa aagttgctct
     1801 tcttaaaata attccgttac tcatttcatt caggttttca ttatatattg ctccatcaac
     1861 ctgaattctg gaatcaaacc accattgaaa atcaccatta tcagaagaaa acagtaattt
     1921 tccatttctt tctttaacac ctaatggaac ttcttgattt ccaccaggaa ttttatcatc
     1981 ctgtgcatac aattcattca ctcctagaat tgaaagcaaa gagattgtga taatgagtac
     2041 aaagtgtttc ttatagttca ttttatctcc tctaagtatg ttgtttaata attattagtt
     2101 ttagttttat tagtaactct gaatagatct tcaattataa ttttcttttc ttcgatcgct
     2161 ttgtcattta gcttatcacc atttagcttc cactcaggta aatttgaata tactgcttct
     2221 tcaaaatcaa ttttatggtt gaaagatcta ccgtgaagtt cttttaattt actcttttga
     2281 gttatagaca attgaaagtt ttcgggtaat tcatttatca gtaatgaatc aattttaaag
     2341 ctgtcaatca aggcgtattt atagattgag taaatatgct tactgttata atcatatctc
     2401 gttttaagtt catttaaaaa agttttgtta tttgtaaatc ttttatttaa aagatcattt
     2461 aatttttcgg ttgcaattcc ttcgtcattt aattttttga ttacagatgt tgtgatttga
     2521 tacggaaccg gatgaataat tttttgttca aatacattct gaataaaaaa caatgaaatg
     2581 aaggtaatca aacctgctgt caaaggagca actacccaac caattccgat tttacctaga
     2641 actttaaaat taattgataa aggatctttg gctagcccaa ctcctataac agctccaata
     2701 aatgcttggg ttgttgaaag tggaactaat ggaaaagatg gtaaattatt ttttaataaa
     2761 aatgtttcta ggctttgaga ggtgaataac cagagcacaa taaattcagc aagtacaaca
     2821 acaaatcctg taattggaga tattttatat aaatcagtac caactgtttc cattacattg
     2881 ttgccataag taaatatacc aatggcaatt gctatcccgc ctaacaagaa taattgctgc
     2941 acacttgaaa tgactatgat gtttgcaata tttagatctt tgaataatgg tgcagaagca
     3001 aacatcccca taacatttgc aatattattt gcccctagtg aatatgaggc taatccacca
     3061 acgataatta atcctattct tgtataatta tccaatcgaa gcagatggat tttccacttt
     3121 aaaactgttt tctttatgag cttaaaaatt aaaaatccaa atattgccgc aataactggt
     3181 gaagcaatcc aactaaataa aatttttgta agtgaattat aatccgtagg agatcctgta
     3241 aaaatattcc agccaattat accacctatt acagcctgtg aagttgagac tggcaattta
     3301 gctctagtca ttaatgttat agaaatacca acagctaaag caaccgagaa acttccagca
     3361 attgcattta tagaacctaa atcagtcaat gtttttgtag taccttcacc acttatcgta
     3421 gcacccagaa taacaaatat tcctgaaatt aaggcagcag ttctaaactt aaccattttg
     3481 gagaccacag cagtgccaaa aacatttaca gcatggttag caccaagcga ccaaccaaga
     3541 aatagaccac tgctaataaa aaaccaaact gaaatatcca atgttattca aaccttttaa
     3601 taatagctat cgagagtctg tcacatacat cttcagactg ttcagcaatt ctttcaatat
     3661 gaaatgtaaa atatcgagta tgaattttaa agctaaggtc attgtctgtt ctaaaaaccg
     3721 ctcgctttat tctttcagca attttatttg tttctttttt ataaaattga actttggtaa
     3781 cataatctct aactgcatca acatttttaa agtaggctct tatgccgctt acagtatttt
     3841 caagacacga tatcgagctt tctgcaatgt cgagataatc attctttagt tcgggtaata
     3901 gatttggaat ctcaacggag aactcaagta aggtttcagc agtaatattt agagctttat
     3961 cgcaactttc catcaatcct aatacgtctc ctcttgattc aggaattaga gttcgggtgt
     4021 aaagtttaat ctctatattt cttcgtaagt catctgcagt ttcctcgatc ttacgtaaat
     4081 cttttaaatg tccttcaaaa gcctctattt gatgatccag gtagcacttg attccaagtt
     4141 tgaatatcaa tcctcctttt attaccagat caagatattc atcaatctgt gtttctaatt
     4201 ttcttgtttt ttttaaaaac ataactctac tatcctttta tctaatttca gtttctttat
     4261 tttgctcttc gtcggaagag gcacaacttc aaaataaata gattaaaaaa ttccgaatac
     4321 cccgtttggt gttattccta aatatctaaa ccatgtttcg ccagcgataa ttataaacaa
     4381 ccaggatatt accatcattg taaatccata tttaaaagta tcagttaaac tgtaatgatc
     4441 agtttcataa agtagtgctg ctggtttact gttaaatggt aatacataga catgttcaat
     4501 caaaaaagcg acgggcaatg ctaagctaat gatatcatat ccaaacctat tggcaacacc
     4561 aattgcaata ggaatgaaaa tcatagcgcg catagttttg gactggaaga taagtgcact
     4621 aaaaaccatt actccggtta acaataaata aagtacccag aattgtgtat agtcaccaat
     4681 acccaagtga tcaaagaaag catttacgct tattgaagga agatcagtta ca
//
LOCUS       DASWBY010000399         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_202081, whole genome shotgun sequence.
ACCESSION   DASWBY010000399 DASWBY010000000
VERSION     DASWBY010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202081"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..132
                     /locus_tag="VF870_07200"
     CDS             10..132
                     /locus_tag="VF870_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252013.1"
                     /translation="MNFKKFPELEASWMYPWGFWIISLVVAIIMIAYFKKKKWF"
     gene            407..1426
                     /locus_tag="VF870_07205"
     CDS             407..1426
                     /locus_tag="VF870_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9252014.1"
                     /translation="MEKYQIVIVGNGEPALVCTLSARNTYSDKSIAVIKTDGQSSIIE
                     EILTSISGRFSNILNVFYGEIVKRDQNVLHLSSGKKIEFEKLVLATGSRAIQPPIDGI
                     QRDGVMLINKDPEQIRNVKFQALDAENVVIYGGGYIGVELCDELLRAGKKVTIIEKSK
                     RLMPSSFDADTSNKVKEVIENLGGKVVLNAKIKSVLGIDAVAGIKLSSDEVLDCDFLM
                     ICCGSRPNVEMAEKIGIIFDRDRGILVDEYFRTSDKNIYAVGDCAAKHDFFASDLSNV
                     LTYNSKMEEAKLLGANLYSVIFNRGKMVSYLSEKKNLKDRIRTELKQLETSNATKDFI
                     PLQRL"
     gene            1670..>2071
                     /locus_tag="VF870_07210"
     CDS             1670..>2071
                     /locus_tag="VF870_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEX9252015.1"
                     /translation="MADNKRPTWDEYFLKLAMLASERATCPRMHCGCVLVKDRFVLAT
                     GYNGSLPGQPHCEDEGCLIVDNHCVRTNHAEINALVQATKHGVNIVGSTAYITNMSCT
                     TCAKALIAAGIKRVVVFSDFHDTLATQFYSDA"
BASE COUNT          716 a          322 c          395 g          638 t
ORIGIN      
        1 gtttatggaa tgaacttcaa aaaatttccg gaattggaag caagctggat gtatccatgg
       61 ggtttctgga ttatttccct cgtcgtggcg ataataatga tagcttattt caagaagaaa
      121 aaatggtttt agttaaacca ttattagaat gactttatat ggttctctat aaaccgcttg
      181 ctattcatct taaaaagttt atattataac cagcgtataa tttctctctc ttgctaatta
      241 atttcttggg ctgatgtaaa atttattgaa accggaaaac ggttttaact taaatttatt
      301 ttgaactcat atttctcatc ccgcttcttt tgagttaaca aacctatccg ttactccggt
      361 tgtaaaacaa tagtataact atgaaaaatg tctaaaggac ctgatgatgg aaaaatatca
      421 aattgtaatt gtcggaaatg gagaacctgc acttgtttgt actttatctg caagaaacac
      481 ctatagtgat aaaagtatcg ctgtaataaa aactgacgga caaagctcaa taattgaaga
      541 gatacttact tccatctcag gtcgctttag taatatttta aatgtttttt atggcgagat
      601 cgtgaagcgg gaccaaaatg tgcttcatct gagcagtgga aaaaaaattg aatttgaaaa
      661 acttgtttta gcaacaggct ctcgtgcaat acaaccaccg attgatggaa ttcaacgaga
      721 tggggtaatg cttataaata aagaccctga acaaattcgc aatgtaaaat ttcaagcatt
      781 ggatgcagaa aatgttgtga tttacggcgg cggatatatt ggcgttgagt tatgtgacga
      841 acttttacgg gcaggaaaaa aagttacgat tattgaaaaa tccaaaaggt taatgccttc
      901 ttcatttgac gctgatacaa gtaataaagt aaaagaagtg atcgaaaatc tgggcggaaa
      961 agtagttctt aatgcaaaaa taaaaagtgt tctcggtatt gatgccgtag caggtataaa
     1021 acttagctcc gatgaagtat tagattgtga ttttttaatg atctgctgcg gctcaagacc
     1081 gaatgttgaa atggctgaaa aaattggaat aattttcgac cgagacagag gaatattggt
     1141 cgacgaatat tttagaacat ccgataaaaa tatttatgct gtaggtgatt gtgcagcaaa
     1201 gcatgatttt tttgccagtg atctttccaa tgttttaaca tataattcaa aaatggaaga
     1261 agctaagtta cttggtgcaa atctttactc tgtaattttt aatcgcggta aaatggttag
     1321 ttatctttcc gaaaaaaaga acttgaagga tagaataaga acagaactta aacaactgga
     1381 gacttcaaat gccacaaaag attttatacc tttgcaaaga ctgtaaaact gagtgggaag
     1441 taaacataac ggcaatacct gcaaattgta ttgtttgtgg aagcagtaaa gtttataaaa
     1501 gtcatcacca tcaaagagct gctaaaaaaa tgcgtagtaa agaaagatgg tcttataaag
     1561 taaaatagtt ttataaactt tttattttca tcattcgcct atctatattt gtctcgcaca
     1621 acaataaatt ttcatattaa ttcttaataa tatttttgga taaaactgaa tggcagataa
     1681 caaaagacca acatgggatg aatatttctt aaaacttgca atgctcgcat cagaacgtgc
     1741 aacttgccca cgtatgcatt gcggatgtgt gttggttaaa gatagatttg tactggcaac
     1801 cggttacaac ggctcacttc ccggtcaacc tcactgcgaa gatgaaggat gtttaattgt
     1861 agataatcat tgtgtccgca caaatcatgc agagataaat gcacttgtac aggcaacaaa
     1921 acatggtgtc aacatcgtcg gatcaacagc atatattaca aatatgtcct gtacaacttg
     1981 cgcaaaagcc ttaattgcag cgggaataaa aagagtagtt gtgttttcag attttcatga
     2041 tacgcttgca actcagtttt attcagacgc c
//
LOCUS       DASWBY010000400         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_653576, whole genome shotgun sequence.
ACCESSION   DASWBY010000400 DASWBY010000000
VERSION     DASWBY010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653576"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /gene="dnaB"
                     /locus_tag="VF870_07215"
     CDS             complement(<1..1301)
                     /gene="dnaB"
                     /locus_tag="VF870_07215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9252016.1"
                     /translation="MAKTSKTYNETSRVIPADVKPPSAPEIEAAVLGAMLIEKEAVPK
                     AIEMLTKESFYLRQHQMIFESMISLFEANEPIDTVTLYEELKKRSQVEEVGGAVYLSK
                     LSQNISSAANVEFHAKIIIEKQILRGLISSSHQIAQAAYEGNLDAFEILDDAERKIFE
                     ITESHLQRSFQGMDRAVRDALEYIEAIHSQKERKFSVPTGFYELDEMLGGFQKSDLII
                     VAARPSMGKTAFALTLARNAAIDHGVPIGIFSLEMATMQLIIRMLCAEGRLNAHLVRT
                     GKLPSEEGVKLSKNAHKLINAPLYVDDSPSQTVLEIRAKARRLKAEKNVGMIIIDYLQ
                     LMQGPPKAESREREISHISRSLKALAKELNIPVIALAQLNRAVEARTDKRPQLSDLRE
                     SGSIEQDADVVIFLNRPEYYGIDKDENGESNEGVAEIIIGK"
     gene            complement(1403..>1837)
                     /locus_tag="VF870_07220"
     CDS             complement(1403..>1837)
                     /locus_tag="VF870_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252017.1"
                     /translation="DFLFVKGEKMKKGLTIFLTALAFYLTRCTDDDSVNPYYYNTPVI
                     SKTTNALAYSLIAEYYTQTAGYNLSFTSDSLAYSLIITNYFSGMGSLRIKDTSGTIIY
                     AETLQGNKVISFTESGNGIPGKFEIDFDRFTGSVNFALAKTE"
BASE COUNT          545 a          364 c          310 g          618 t
ORIGIN      
        1 tgcttgccga taattatttc cgcaacacct tcattcgatt caccattttc atccttatca
       61 attccgtagt actcaggacg gtttaaaaag attacaacat ctgcatcctg ctcgattgaa
      121 cctgattcac gaagatctga aagctgcgga cgtttatctg ttctggcttc aacagcacga
      181 ttaagctgtg caagtgcaat aacgggaatg tttaattcct ttgccagagc ttttaatgat
      241 cttgaaatat gtgaaatctc cctttcacga ctttcagctt ttggcggacc ttgcatcaat
      301 tgaaggtaat ctataattat catcccaaca tttttttcag cttttaatct tcttgctttt
      361 gcccgtattt ccagaacagt ttgcgatgga gaatcatcaa catacaatgg tgcattaatt
      421 aacttgtgag catttttact aagcttaaca ccttcttcgc ttggcagttt tccggttctt
      481 acaagatgcg catttaatct gccttcagcg caaagcatac gaattattaa ttgcatcgtc
      541 gccatttcaa gactaaaaat tccaatcgga actccgtgat caattgcagc atttcgggcg
      601 agtgttaatg caaacgctgt ttttcccatg gatggtcttg cagcaacaat tatcaaatct
      661 gatttttgaa aaccacccag catttcatcc aactcataaa atcctgttgg aacagaaaat
      721 tttctttcct tttgtgaatg aattgcttca atgtattcaa gagcatctcg taccgcacga
      781 tccattccct ggaaagatcg ctggagatga gattctgtta tctcgaatat tttcctttct
      841 gcatcatcaa gaatttcaaa tgcatccaaa tttccttcat aagcagcttg cgcaatttgg
      901 tgtgatgaag aaatcaatcc gcgtaagatt tgcttttcta taattatctt agcgtgaaat
      961 tccacatttg cagcggaaga aatattttgt gaaagtttac ttaaataaac cgcaccgccg
     1021 acttcttcaa cctgacttct ttttttaagc tcttcgtaca aagtgacagt atcaatcggc
     1081 tcgttagctt caaacaatga aatcatagat tcaaaaatca tttgatgctg acgaagataa
     1141 aaactttcct tagtaagcat ttctattgct ttaggaacgg cttccttttc gataagcatt
     1201 gcaccaagaa cagcggcttc tatttcagga gcggatggtg gttttacatc agccggaata
     1261 acccggctcg tttcgttata tgttttactt gtttttgcca taatcaaaaa aatatcccga
     1321 ttgaagcggg atgtaaaagt aacttactta gaatttatat acaagatacg aaagggacga
     1381 attactgact ttttcgttaa ttctattcag tcttcgccag agcaaaattc acagatcctg
     1441 tgaagcgatc aaaatctatt tcaaactttc ctggaatacc gtttcctgat tctgtaaaag
     1501 aaattacttt atttccctgc agagtttctg cataaatgat tgtgccgctg gtatccttta
     1561 ttctaagtga acccatgcct gaaaaatagt ttgtaattat tagtgagtat gcaagggagt
     1621 cagaagtaaa acttaaatta taacctgcgg tttgagtata atattctgca attaaagaat
     1681 aagccaatgc atttgttgtt tttgatataa ccggagtgtt ataataatat ggattaacag
     1741 aatcatcatc tgtgcatctg gtaagataaa atgctaatgc tgttaaaaag attgttaatc
     1801 cttttttcat tttttctcct ttaacaaata gaaaatc
//
LOCUS       DASWBY010000401         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_580115, whole genome shotgun sequence.
ACCESSION   DASWBY010000401 DASWBY010000000
VERSION     DASWBY010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580115"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VF870_07225"
     CDS             complement(<1..375)
                     /locus_tag="VF870_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252018.1"
                     /translation="MKKIFILYIVISSTIFAQSFNYTESIGKFKKASSFYITANGLIY
                     VSDSGSDEIVLLDTLGNVLKTFGGYGWDQNSFDDPADVFADPLTIYVADKNNHKIKRF
                     DKNLNFIYALYTRDSDIQEEQFG"
     gene            509..581
                     /locus_tag="VF870_07230"
     tRNA            509..581
                     /locus_tag="VF870_07230"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:542..544,aa:Gln,seq:ctg)
     gene            675..748
                     /locus_tag="VF870_07235"
     tRNA            675..748
                     /locus_tag="VF870_07235"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:709..711,aa:Glu,seq:ctc)
     gene            769..1140
                     /locus_tag="VF870_07240"
     CDS             769..1140
                     /locus_tag="VF870_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX9252019.1"
                     /translation="MSAFLILEVMKRYNKHIFICENKRPEGHPRGCCSEKGSAEIREY
                     FKLRLKELGLNAEVRANSAGCLDACEFGVSVVVYPEQIWYGAVTKNDVDEIIQSHIVN
                     NNPVERLKITHPRYNKDASKA"
     gene            1124..>1528
                     /gene="nth"
                     /locus_tag="VF870_07245"
     CDS             1124..>1528
                     /gene="nth"
                     /locus_tag="VF870_07245"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9252020.1"
                     /translation="MQAKLSKDFAKQIFDILKKEYPKVKPALVYSNPFELLISTILSA
                     QCTDERVNIVTKELFKKYKKPQDFINLSQEQLEKEIFSTGFYKQKAKSIKACCDELIK
                     NHNGKVPADFDELVKLSGVGRKTASVIAGNAFG"
BASE COUNT          532 a          240 c          271 g          485 t
ORIGIN      
        1 accaaattgt tcttcctgaa tgtcgctgtc gcgcgtataa agtgcataaa tgaaattaag
       61 atttttatca aatcttttta ttttatgatt atttttatct gcaacgtata ttgtcagggg
      121 atccgcaaaa acatcggccg gatcatcaaa agaattttga tcccaaccat aaccgccaaa
      181 agtttttagt acattgccta atgtgtcgag taaaacaatt tcatcactac cggaatccga
      241 aacataaatt aatccatttg cagtaatata aaaggaggaa gcttttttaa actttccaat
      301 cgattctgtg taattaaaac tttgagcaaa aattgttgaa gaaattacta tgtaaagtat
      361 aaatatcttt ttcattctaa ataaatctta attgttagtc taaattaagt taagatttct
      421 gccaatctca agtagtttcg gcatcttaaa actgattgca tttagaattg aattcagata
      481 tatttggcat caaaattaag taataaattg tcccgtggtg taattggcaa cacgtctgac
      541 tctggatcag aagagttcag gttcgaaacc tgacgggaca acaaaccaat gtacaatgaa
      601 agatttagaa tttacaatta aagcattttt aattgtgcat tgtacattgt gaattgtaaa
      661 cttcatttta cattggcgcg ttcgtctagt ggcttaggac accgccctct caaggcgggg
      721 atcacgggtt cgaatcccgt acgcgctaca aaaaaaatag cggacttaat gtccgctttt
      781 cttattttag aagtaatgaa aagatataat aaacatattt ttatttgtga aaataaaaga
      841 cctgaaggac acccacgagg ttgttgttct gaaaagggta gtgctgaaat acgtgaatac
      901 tttaaattac gattgaaaga attagggcta aacgctgaag ttagagctaa ttctgcggga
      961 tgcctggatg cttgtgaatt cggtgtatct gttgttgttt atcctgaaca aatttggtat
     1021 ggtgctgtaa ctaaaaatga tgttgatgaa attattcaat ctcacattgt taataataat
     1081 cccgtggaaa gattaaaaat aacccatccg agatataata aagatgcaag caaagcttag
     1141 taaggatttt gcaaaacaaa tatttgatat tcttaaaaaa gaatatccaa aagtaaaacc
     1201 tgcacttgta tattcaaatc cttttgaact gcttatctct actattcttt ctgctcaatg
     1261 tactgatgag cgagttaata ttgttaccaa agaacttttt aagaaataca aaaagccgca
     1321 ggattttatt aatctttccc aagagcaatt agaaaaagaa atattttcaa ccggatttta
     1381 taaacaaaag gcaaaaagta ttaaagcttg ctgtgatgag ctgattaaaa atcataatgg
     1441 aaaagttcct gctgattttg acgaacttgt aaaactttcc ggtgtaggca gaaaaacagc
     1501 ctcggtaatc gccgggaatg catttgga
//
LOCUS       DASWBY010000402         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_730222, whole genome shotgun sequence.
ACCESSION   DASWBY010000402 DASWBY010000000
VERSION     DASWBY010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730222"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2306
                     /locus_tag="VF870_07250"
     CDS             <1..>2306
                     /locus_tag="VF870_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9252021.1"
                     /translation="IDIKSKLTRLETESELNQILKLLDEISVLAFTKSFSIRKKGYLK
                     GEIAESISNEIKSAEDLMKSIHAFQVPENHKQIELELAKFGKTLLTFFEFALNIYEDK
                     KKNEGFIDYEDILLHTKLLLENADVQKAISSTYKFIMVDEFQDTNEIQYQIFLPILDY
                     LKAGRLFIVGDEKQSIYKFRDAEIEIFNLTRSDIKNSVGEENLLILPDSFRMTPVICA
                     FCNYVFKNLFNDPDESLGEVPATDLVCARIDDIKGHVEFLVSRTDDENPGDSEAELVA
                     RKILFLLKNSNYSYKDISILVRKRKYFADLEKVFLKYDVPFTIVGGRGFYQRQTIADV
                     FNYLSFLADENNSSALVGLLRSPFFNISDSKLYEISLNKGRSFWRKLNLVKDDSEISK
                     ICELLSENISICSSVSLPDLIHKIITDRNFLSILSSRNDSDQEIANLNKLVSIARNFN
                     SVGFRNLYDFLAFIKESITNLSDESQASISSNTNAVQMMTIHQSKGLEFPVVFLYKTD
                     EAGMSSTIKSGEVKVDKKFGLLTKVPVDQNYFEEYQTAPIAIMHNYFEDRKNNAELKR
                     LLYVALTRTKDELYITSTIKKDDDFKKDSFINLLASGLKNNFSGNVIVFSTQLETLKF
                     DNDRFVNKNQKINLNIPITSDIELEINEGIEEVIDADKAEINTTSLHSHEKGEIISAS
                     KVSIYSQCPLKYLLTYEYGFGKSNTDYLNFKFLDKQKNQKYFSDSVLEDEIEDDNERF
                     ESLGSQDKNYDSALYGRLFHKAMEKSFYP"
BASE COUNT          836 a          335 c          395 g          740 t
ORIGIN      
        1 atagatatta aatctaaact tacaagatta gaaacagaat ctgagcttaa tcaaatatta
       61 aaattattgg atgaaataag tgtattagca tttaccaaga gttttagtat tcgaaaaaag
      121 ggttacttga aaggtgaaat agctgaatca atctcgaatg aaattaaatc agccgaagat
      181 ttgatgaaaa gcattcatgc ttttcaagtt cccgaaaatc ataaacaaat tgaattggaa
      241 ttggcaaaat ttggaaaaac acttctgact ttttttgaat ttgcgcttaa tatctatgaa
      301 gacaaaaaaa agaatgaagg atttatagat tacgaagaca ttcttctcca tactaaacta
      361 ctattagaaa atgcagatgt acaaaaagca atttcttcta cgtacaaatt tattatggtt
      421 gatgaatttc aggatacaaa tgaaattcaa tatcaaatat ttcttccaat tctggattac
      481 cttaaagctg gaagattgtt tattgtcggc gatgaaaaac aaagcatcta caaattccgc
      541 gatgctgaaa ttgaaatatt taatcttacc agaagcgata tcaaaaattc ggtgggggaa
      601 gagaatcttt taattcttcc ggatagtttt agaatgaccc cggtaatctg cgctttttgt
      661 aattatgtat ttaaaaattt atttaatgat cctgatgaaa gtctgggtga agttcctgct
      721 acggatttag tttgtgcccg cattgatgat atcaaaggcc atgttgaatt tttagtttcc
      781 aggacagatg atgaaaaccc tggcgattca gaagctgaat tagttgccag gaaaatatta
      841 tttcttttaa aaaacagcaa ctactcttat aaagatattt caattcttgt tagaaagaga
      901 aagtactttg ctgatcttga aaaagtattt ttaaaatatg atgtaccctt tacaattgtg
      961 ggcggaagag gattttatca aagacaaact atagctgatg tctttaatta cctgtctttt
     1021 cttgcggacg aaaataatag ttctgcatta gtcggattat tgcgctcacc attttttaat
     1081 atttcagatt ccaaactata tgaaatatct ttgaacaaag gcaggagttt ttggagaaag
     1141 ctaaatctgg taaaggatga tagtgaaata agtaaaattt gtgaattact gagtgaaaat
     1201 atttcaatct gcagttcagt tagcctgcct gatttaattc acaaaataat aacagacaga
     1261 aattttttat caattctttc atcccgaaat gacagcgatc aggaaattgc aaatcttaat
     1321 aagcttgtaa gcattgccag aaattttaat tcagttggat tccggaatct ttatgacttt
     1381 ttagcgttta taaaggaatc aattacaaat ctgtctgatg aatcccaggc ttcaatttca
     1441 tcgaatacaa atgcagttca aatgatgacg attcatcaat caaagggact ggaatttcct
     1501 gttgtgtttt tgtacaaaac cgatgaagcc ggaatgtcca gcacaataaa atccggcgaa
     1561 gttaaagttg ataaaaaatt tggccttctt acaaaagttc cggttgatca aaactatttt
     1621 gaagaatatc agactgcgcc gattgcaata atgcacaatt attttgaaga tagaaaaaac
     1681 aatgcagagc ttaaacggct tttatatgtc gcattaactc gcaccaaaga tgaactttat
     1741 attacctcaa caattaaaaa agatgatgac tttaaaaagg attcttttat aaatctatta
     1801 gcctcgggat taaaaaataa tttttcaggt aacgtaattg ttttcagcac ccaattagag
     1861 acattaaaat ttgataatga tcgctttgta aataagaacc agaagataaa tctcaacatt
     1921 cctattacat cagacataga gttggaaata aacgaaggaa tagaagaagt tattgatgct
     1981 gataaagctg aaatcaatac aacctcattg cattcacatg agaaaggaga aataatttcc
     2041 gcttccaagg tttctatcta tagccagtgt ccgttaaagt atctgcttac atatgaatac
     2101 ggttttggaa agtctaacac agattatttg aattttaaat ttttagacaa gcagaaaaat
     2161 caaaaatatt tttctgattc tgttcttgaa gatgaaattg aagatgataa tgagcgattt
     2221 gaatctctcg gcagtcagga taagaattat gattctgcgt tatacggtag gttatttcat
     2281 aaagcgatgg aaaaatcttt ttatcc
//
LOCUS       DASWBY010000403         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_352345, whole genome shotgun sequence.
ACCESSION   DASWBY010000403 DASWBY010000000
VERSION     DASWBY010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352345"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VF870_07255"
     CDS             <1..854
                     /locus_tag="VF870_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX9252022.1"
                     /translation="GGLIFNGRVSITNGKFSENFIVPKDISYENKNGKVILYFLDNSV
                     DGLGYTNKIIVGGTDSTLANDGKGPEIEIYFDDVANTNGYLVNQNPSLIVKLSDETGL
                     NTTGTGVGHKLEGILNQQLNNPIDFTNHFTGDLDAGGRTGSINYKFSALSNGDYELLV
                     KAWDVFNNFSEENTFFSVVDGSDLEIRDVYNYPNPFSDRTQFTFQQNLTQPIDVKIKI
                     YTIAGRLIKEIEKNNLNEKFAVIDWDGRDADGDPLANGTYLYKLIVKSVDGEFSKSVL
                     GKLAVIK"
     gene            960..2117
                     /locus_tag="VF870_07260"
     CDS             960..2117
                     /locus_tag="VF870_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9252023.1"
                     /translation="MKIVLRLMMISMLFGVFARVSYAQGEAAVPFLLLAPDSRAGGIG
                     ESGAGLADNSAAIFWNPAGIAFLTGTEVSITHSNWLPQFNLDLFYDYLTYRQYIEDIS
                     GSITASITYMNFGEFVRTAEDSPDPIGTFRSFDAALTLGYATKLGTDWGLGFNFRLIH
                     SRLSDQPTAEEQGSGVATSVSFDIATMWRPEKFVLPLIDEDIGGKLSIGANLSNLGPK
                     IYYIDKAQADPIPTNFRLGFAYKIIDDEFNNLVYTLDFSKLLVSRDSTGSDEFYQAII
                     SAWGDESFNNELRDIVTSMGLEYVYGTPGDFLFALRTGFFYEDPSYGNRKFLTFGAGI
                     RYDIYGFDFSYITTSVFSNGENHPLSDTLRFTVLIGWGAIPESTRGLPRGI"
     gene            2124..>2664
                     /locus_tag="VF870_07265"
     CDS             2124..>2664
                     /locus_tag="VF870_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252024.1"
                     /translation="MLKNVLTLAISLITLGSISAQSFIGKINPYPNSNSSVLADDTVK
                     IFAVMVSFQEDRDGATFGNGKFGSIYSQNYGNDILDPLPHDKDYFESHLTFVKNYFQK
                     VSNGKVNIQFTVLPDTFSVTKTMRNYSPAPGSDNFTPMGQFAEEVWTKADQMYPGLNF
                     SDYNLFVIFHAGVGRDISLP"
BASE COUNT          839 a          413 c          527 g          885 t
ORIGIN      
        1 cgggcggatt gatttttaac ggaagagtat ccataacgaa cggaaaattt tcggaaaact
       61 ttattgttcc aaaggatata tcatacgaaa ataaaaacgg taaagtaatt ttatatttct
      121 tagacaactc cgtggatgga cttggatata ctaataaaat tattgttggc ggaacggata
      181 gtactttagc aaatgatgga aaaggtccgg aaatagaaat ttattttgat gatgttgcca
      241 atactaacgg atatcttgtt aatcaaaatc ctagtttaat tgttaagctt tctgatgaaa
      301 ccggattgaa tactaccggc accggtgttg gtcataaatt agaagggatt ttaaatcagc
      361 agttaaataa cccgattgat ttcacaaatc attttaccgg tgatttggat gcgggcggta
      421 gaacaggatc gataaactat aagttctcag ctttatctaa tggtgattat gaattgcttg
      481 taaaagcttg ggatgtcttt aataactttt cagaagagaa tactttcttt tctgttgttg
      541 atggaagtga tcttgaaata agagatgttt ataattatcc taatccattt agtgatagga
      601 cgcaatttac atttcagcaa aacttaactc agcctattga tgtaaaaata aaaatctaca
      661 caatagcagg cagactgatc aaagaaattg agaaaaacaa tctaaatgaa aaatttgctg
      721 ttattgattg ggatggaaga gatgcagatg gagatccgct tgctaacgga acttatcttt
      781 ataaattgat tgtaaaatct gttgatgggg agtttagcaa gagcgtgctt ggtaaacttg
      841 cggtgattaa ataatccgct ataaaaaaat gtgttttata tatgtgttat tagttatatt
      901 tattattcaa acttgattag aattttttta attatcttat ttatctcgga ggataaaaaa
      961 tgaaaatagt tctcagactt atgatgatca gcatgctgtt tggagttttt gccagagtat
     1021 cgtatgcaca aggtgaagct gctgttccat tcttattact tgcgccggat tctcgtgccg
     1081 gtggtatcgg tgaatcaggt gccggattgg cagataattc tgctgcaata ttctggaacc
     1141 ctgcaggtat cgcattttta acaggcactg aagtcagcat tacacacagt aattggcttc
     1201 cgcagtttaa cctggattta ttttacgatt accttaccta ccgccaatat attgaagata
     1261 tcagcggaag tattacagca agtataactt acatgaattt tggtgaattc gttagaaccg
     1321 ccgaagatag tccggatcca attggcactt ttagatcttt tgatgcggca cttacattgg
     1381 gttatgcaac taagcttggt accgattggg gattaggatt taactttaga ttaattcata
     1441 gccggttatc tgatcagcct actgcagaag aacaaggcag cggcgttgca acatcagtaa
     1501 gttttgatat tgcaacaatg tggcgtcctg aaaaatttgt tcttccatta attgatgaag
     1561 atattggcgg caaactcagt atcggggcta acctaagtaa tcttggtcca aaaatttatt
     1621 acattgataa agcacaggca gatcctatcc ctacaaactt tagattaggt tttgcttaca
     1681 aaataattga tgatgaattt aataatcttg tttatacatt agattttagc aaacttttag
     1741 tcagcagaga tagtaccgga tccgacgagt tctatcaggc aattatttcg gcatggggag
     1801 atgaatcttt taataatgag ttaagagata ttgtaacatc aatgggtctg gaatatgtgt
     1861 atggaactcc gggcgatttt ctatttgcac ttcgtacagg atttttctac gaagatccat
     1921 catatggtaa cagaaaattc ttaacttttg gtgctggtat cagatatgat atttatggat
     1981 ttgattttag ttatataaca acttcagtct tttcaaatgg tgaaaaccat cctctctctg
     2041 atacacttag atttactgtt cttataggtt ggggtgcaat tccggaatca acaagaggat
     2101 taccaagggg aatctaactt cttatgctaa aaaatgttct aactctcgca atcagtttaa
     2161 taactttggg cagtatatct gcccaaagtt ttattggaaa aattaatcca tatccaaatt
     2221 ccaactcatc agtgcttgca gatgatactg ttaaaatttt tgctgtaatg gtaagctttc
     2281 aggaagatcg tgacggcgca acatttggaa atggaaagtt tggttcgatt tattctcaga
     2341 actatggtaa tgatatatta gatcctttac cacacgacaa agattatttt gaatcacacc
     2401 tgacatttgt taaaaattat ttccaaaaag tttccaacgg aaaagttaat atacagttta
     2461 ctgtattacc ggatacgttc tctgttacaa aaacgatgag aaattattct cccgcacccg
     2521 gttctgataa ctttactcca atgggacaat ttgctgaaga agtttggaca aaagctgatc
     2581 agatgtatcc tggattgaat ttttcagatt ataatttgtt tgtgattttt catgccggtg
     2641 ttggaaggga tatttcttta ccag
//
LOCUS       DASWBY010000404         1836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_277968, whole genome shotgun sequence.
ACCESSION   DASWBY010000404 DASWBY010000000
VERSION     DASWBY010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277968"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..991
                     /locus_tag="VF870_07270"
     CDS             92..991
                     /locus_tag="VF870_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9252025.1"
                     /translation="MTLQIENVTKKFGDFSAVKKLTMTVQQGSMYGFLGANGAGKTTT
                     FRMILGLLDTTEGSITWNGNPITYGNSSHIGYLPEERGLYPKMKVEEQLVYLGQLRGM
                     KKNDAKIASNEWLERFEVPHYAHKKVEELSKGNQQKIQLIASLLHKPSLIILDEPFSG
                     LDPVNVEMLKKAILDIQKEGATIVFSSHRMDHVEELCEQLCIMHHGEAIVSGSLREVK
                     RGFGRQNVRIKADFDLTFLGEMEGVNSFKSSIEGGLFQISQERTAHELLRTALTKGDI
                     RYFAIEEPSLQDIFIEKVGKEHA"
     gene            984..>1836
                     /locus_tag="VF870_07275"
     CDS             984..>1836
                     /locus_tag="VF870_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9252026.1"
                     /translation="MLDFWTIFKQSFWTKAKTKAFVITTLIVVAGIFLMANMSTIISK
                     VQEVGVFGDGANEDIIYVIDDSGSLVDQLQQQLDVKESGITLKATTDSIQDLENQITE
                     EKISSLLVLGFNDADTIQATYTSKNANDFEQPMSIREALQNVQTNLKANTLKLTGDEV
                     AQLFAPIQFEQEAVSASSKSEEELSQARGLVYVLIFVIYFAVFYYSNMIAMEVATEKS
                     SRVMEIIISSVSPVKHMFAKIAGVGTLGLLQIALFGVVGFVALKTSSSDLTSGVFSFF
                     GFSNVSIS"
BASE COUNT          623 a          289 c          391 g          533 t
ORIGIN      
        1 atatccaact taataggaat gaattgccaa ataagttgat attataaatc agtagaataa
       61 ttgtcgagat aaatttttaa ggagtgaaaa catgacatta caaattgaaa atgtgacaaa
      121 gaaatttggt gatttctcag cagttaagaa gttaaccatg actgttcagc aaggaagtat
      181 gtatgggttt ttaggagcga atggtgcagg taagacaact acatttcgca tgattttagg
      241 gttactagat actaccgaag gctctatcac ttggaatgga aaccccatta cgtatggaaa
      301 tagttcacat atcggttact tacctgagga acgaggtttg tatccgaaaa tgaaagtaga
      361 agagcaatta gtatatttag gacagctccg tggtatgaaa aaaaacgatg caaagattgc
      421 gtcaaatgag tggttagaac gttttgaagt gccacactat gctcataaaa aagtcgaaga
      481 attatcaaaa gggaatcaac aaaaaataca actcattgct tcactgttac ataagccatc
      541 cttaatcatt ttagatgaac catttagtgg gttagatcca gtcaatgtag aaatgttgaa
      601 gaaagccata ttggacatac aaaaagaagg ggcaacgatt gtattctcca gtcaccgaat
      661 ggatcatgta gaagaattgt gtgagcaact gtgcattatg catcatggag aagccattgt
      721 cagtggttcg ttacgggagg tgaaacgcgg atttggacgc caaaatgtca gaataaaagc
      781 agatttcgac ctaactttcc taggggaaat ggaaggggtc aattcattta aatcttctat
      841 tgaaggtggc ctctttcaaa tctcacaaga acgaacggca catgaactat tacgcacagc
      901 gttaacaaaa ggggatattc gttattttgc aattgaggaa ccatcgcttc aagacatttt
      961 cattgaaaag gtggggaaag aacatgcttg atttttggac gatatttaaa cagtcttttt
     1021 ggactaaagc aaaaactaaa gcatttgtca taacgacatt aattgtagtt gcaggtatat
     1081 tcttgatggc aaacatgtct actattattt caaaagtaca agaagtgggc gtttttggag
     1141 acggagcgaa cgaagatatc atttacgtaa tcgatgatag tggtagtctc gttgatcaac
     1201 tgcagcaaca gttagatgtg aaagaaagtg gaatcacatt aaaagctaca actgattcta
     1261 ttcaagatct ggagaatcaa attacagaag aaaaaatttc gagtttactg gttttaggat
     1321 ttaatgacgc tgacactatt caagcaacat acacgtcaaa gaatgccaat gattttgagc
     1381 aacccatgtc cattcgtgaa gctcttcaaa acgtccaaac aaaccttaaa gcgaatactt
     1441 taaaattaac aggggatgaa gttgcacagt tatttgcacc tatccaattt gaacaagaag
     1501 cagttagtgc ttcttcaaaa tcggaagagg aattaagcca agcacgtggt ttggtttatg
     1561 tgttaatctt cgttatttat ttcgctgttt tctactattc aaatatgatt gcgatggaag
     1621 tagccacaga gaaatcatca cgggtaatgg aaatcattat ttcaagtgtg tccccggtta
     1681 agcatatgtt tgcgaaaatt gccggagtag gaactcttgg gttattgcaa attgcattat
     1741 ttggagtggt gggttttgtc gcattaaaaa caagttcatc tgacttaaca tccggtgtgt
     1801 tttcgttctt tggtttctca aacgtcagca ttagta
//
LOCUS       DASWBY010000405         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_730527, whole genome shotgun sequence.
ACCESSION   DASWBY010000405 DASWBY010000000
VERSION     DASWBY010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730527"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1130
                     /gene="lepB"
                     /locus_tag="VF870_07280"
     CDS             186..1130
                     /gene="lepB"
                     /locus_tag="VF870_07280"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9252027.1"
                     /translation="MSFQEKLKNFFKKKEKPKPTTISGKIAEFFKQLAFAGIAALFII
                     TFIIQNTRIPTGSMEDTILVGDFVLVNKFIYGSSSPRYIPFTEIALPYFTLPAFKEPK
                     AKDIVVFEYPGDRDQLEATEKNINYVKRCIGTPGDTVEVIDKVVFVNGKQFWIPPFIK
                     YYRGQYGSYLRPVPKGVAEPRIFPKGMPWNEDNYGPLVIPKKGMKIALNIYNVEQWRT
                     IIDREYGKRVVEIKNNSVAIDGIPVSSYTFKKDYYFMMGDNRDDSLDSRFWGLVPRDA
                     VVGEAFITLFSWDRDIPFTEFFKLLGSIRPDRVLKLLH"
     gene            1377..>2736
                     /locus_tag="VF870_07285"
     CDS             1377..>2736
                     /locus_tag="VF870_07285"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9252028.1"
                     /translation="MGANVYLKTLDIGGVTNTNGEYSIDKIPGGTFEVTISFVGYVPL
                     TQNINFDGNKVFNFTLKQSPVLLQEAVVQGTRATLRETPVAFTQIEGETIEFKLASRD
                     LPQVLNVTPSVYSSPGGGGAGDANLVVRGFNQRNIAIMINGVPVNDMENGWVYWSNWA
                     GLGEVTQDIQIQRGLGASPYSVSSIGGVVNILTYGASGRKEFSKLRQEVGSDGLYKTT
                     FAFSSMLSDKVGMTALISKKVWDGYANGLYLDEFTYFLSVGGVFGDHSLELTGVGSPQ
                     EHGQRTTRLTIDEWNQRGFDYSRNYGTLFNDPYNEVVNKYHKPQWNLNWNWQLSPKSV
                     LSTIAYFSWGNGYGSGRLGSNWIYNDAGEIDFDAVWARNDASTNGSTTILRNSVNNHF
                     WTGLLSTFKTNLSDSWDLSFGVDGRYYLGQHYREIRSLLGGDYWLNTKDKNNPNQITK
                     TGD"
BASE COUNT          927 a          416 c          516 g          877 t
ORIGIN      
        1 atacagattg tatggattac ttcataagta tactatttgt catttcgacc agaggaggaa
       61 atctttttat cattgaaata cagatttctc agtcgaagac tccttcgaaa tgacaaatct
      121 aaatgtagca actaatccta atatctttgt aagaatttta aattaaaaat aatggagaaa
      181 caaaattgtc atttcaggaa aaactaaaga acttttttaa gaaaaaagaa aaaccaaaac
      241 caaccacaat aagtggtaaa attgcagagt tctttaaaca gttagcattt gcaggaattg
      301 ctgcactttt tataattact tttataatac aaaatacacg catcccaaca ggatcaatgg
      361 aagacacaat acttgttggt gattttgtgc tggtaaataa atttatttac ggatcaagct
      421 cgccacgcta tattcctttt acagaaatcg cacttcccta ttttacgctt cccgcattta
      481 aagagcctaa agcaaaagat atagttgtgt ttgaatatcc tggtgatcgc gatcagcttg
      541 aagcgacaga aaaaaatatc aactatgtaa aaagatgcat cggcactcct ggcgacactg
      601 tggaagtaat tgataaagtt gtgtttgtaa atggtaaaca attctggata ccacccttta
      661 taaaatacta tcgcggtcaa tatggaagtt atctcagacc tgttccaaaa ggtgttgcag
      721 aaccgagaat ttttcctaaa ggtatgcctt ggaatgaaga caattatggc ccattggtaa
      781 tccccaaaaa agggatgaag atagctctga acatttataa tgttgaacaa tggaggacca
      841 tcattgatag agaatacggc aaaagagttg ttgaaattaa aaacaattct gttgcaatag
      901 atggaattcc tgtttcatca tacacattta aaaaagatta ctactttatg atgggtgata
      961 acagagatga tagtttggat agccgattct ggggattagt tccaagagat gcagttgttg
     1021 gagaagcatt cattactctt ttttcatggg atagagatat tccatttaca gaattcttta
     1081 aacttttagg atcaataaga ccggatagag tcttaaaact tttacattaa attatgattg
     1141 aaatgttgat tcttaataat ttgtcgccaa atatcaaaat tattttattg taaaattatt
     1201 aaaatcaact tgtataatta gctacaaatt ctaatttttc atttgtcaaa taattataaa
     1261 aggtcataat atgaaaaaac tattttttac tgtttctact tttttcactt ccaatttttg
     1321 ctcaacagta ctctcttaag ggtaaagtta ctgatgctca atcaggtgaa gcacttttgg
     1381 gagctaatgt ttatttaaaa acattagata tcggcggtgt tacaaatact aacggcgagt
     1441 acagcattga taagattcca ggtggaacat ttgaggtaac aattagtttc gtgggttatg
     1501 taccattaac tcagaatatc aattttgatg gtaataaggt gtttaatttt actttaaagc
     1561 aatcccctgt tctacttcaa gaagctgttg tacaaggtac aagagctact ctaagagaaa
     1621 ccccagttgc cttcacacaa attgagggag aaactattga atttaaatta gcttctcgtg
     1681 atcttcctca agtattaaat gtaactccta gtgtttactc ttcaccgggt ggtggtggtg
     1741 ctggagatgc aaacttggta gtccgtggct ttaaccagag aaatatagct ataatgatta
     1801 atggtgtacc tgttaatgac atggaaaacg gttgggtata ctggtcaaat tgggctggct
     1861 taggtgaagt tactcaagat attcaaattc aaagaggact tggagcatcc ccttattctg
     1921 tttcctcaat tggcggtgtt gttaatatat taacatatgg cgctagcgga agaaaagaat
     1981 tttctaagtt gagacaagag gtcggttcag atggtcttta taaaactact tttgcatttt
     2041 catctatgct aagtgataag gttggaatga cagctttaat ttcaaaaaaa gtttgggatg
     2101 gatacgcaaa tggactatat ctagatgaat ttacatactt cttatccgtt ggtggagtat
     2161 ttggggatca ttcattagaa ttaactggtg ttggttcacc acaagaacat ggtcaaagaa
     2221 ctacaagatt aactattgat gaatggaatc aaagaggttt tgattatagc agaaattatg
     2281 gtacactttt caatgatcct tataatgagg tagtcaataa ataccataaa ccgcagtgga
     2341 acttaaactg gaattggcag ttaagtccaa aatctgtttt atctacaata gcttatttct
     2401 cttggggtaa tggatatgga tcgggcagat taggatctaa ttggatctat aacgatgctg
     2461 gggaaataga tttcgatgct gtttgggcaa gaaatgatgc aagtacaaat ggatcaacca
     2521 caattcttag aaattcagtg aacaaccact tttggacagg tttactttca acattcaaaa
     2581 caaacctttc agattcctgg gatttaagtt ttggagttga tggcagatat tatcttggtc
     2641 agcattatcg tgagatacga tctttacttg gcggtgatta ctggttaaat acaaaagata
     2701 aaaataatcc aaatcaaatt actaaaactg gtgact
//
LOCUS       DASWBY010000406        10859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_615320, whole genome shotgun sequence.
ACCESSION   DASWBY010000406 DASWBY010000000
VERSION     DASWBY010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615320"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VF870_07290"
     CDS             <1..850
                     /locus_tag="VF870_07290"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEX9252029.1"
                     /translation="EYNTGKLKKNDYWAPTYEDGLNLLAKLPGIAAFIYRLKYKNGEL
                     IDSDPKLDLGGNFAHMMGIAKPYDDVARMYFILHSDHESGNVSAHTGHLVASALSDLY
                     YSISAMTNGLAGPLHGLANQEVLAWLQGVYDKMGGKVPTEEEMRQFVWDTLKGGQVIP
                     GFGHAVLRKTDPRYMAQREFCLKHLPNDPLFKYVDLLFKVVPPVLLEQGKAKNPWPNV
                     DAQSGVIQWYYGLKEYDFYTVLFAVGRALGVVANIIWDRGLGYPIERPKSVTTAMLEE
                     AAGIKN"
     gene            1302..6083
                     /locus_tag="VF870_07295"
     CDS             1302..6083
                     /locus_tag="VF870_07295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9252030.1"
                     /translation="MTNEPVSYLTEINDKLLAGKASLEQNEIDDLLVNLRIELNRAIS
                     KKQLGSDTDTGAWQNIFDGLFSSELANPFMENMDKDLFFSFGEYLASLSNEPSERNVS
                     ITHNYLNLFRNSSFLHKIYDERRWDNLIHSLIVQSNYTFDVLFNQRVNQYKKKNLFRI
                     IKDSRTIDYSWQKTSEIVSTYRSSIHHILFEIESESKIVAFLLENSLEMVMLDLACLT
                     SGIVNAMIPANSVSEHISFILNQTKAPILFADDEKQLAKIRSIKKETPFLKTVVMLKG
                     NAAEDWVISFEEFKAMASDHSIKTDVQIKPDSLATLMYTSGTTGEPKGIMFSHTNIVY
                     KRFCRAMAIPGISDEDRYLAFLPLYHTFGRWLEMTGAIFWGAEYCFMENPSVETMIEN
                     MRHVKPSIFISIPKKWIQLYEYITARIDVEVDDDEKIKEAVVSATGGKLKWGLSAAGF
                     LPPDIFRFLQRYGIELMSGFGMTEATGGITMTPPFQYRENSLGKALPGIEIKLGEDGE
                     ILIRGPYVMLNYYGQKLEDTFDADGWLATGDVMRMDRDGFIEIIDRKKEIYKNIKGET
                     IAPQKIENLFRDFEFVKQVFLVGDHRPFNTVIIFPDIESEISPLNQMDEQQRQEYIST
                     LIVSVNNFLAPFERILDFRIITRAFDDANGELTPKGTYKRRVIEKNFDDLIRSMYIKD
                     HLSLVLDKYEIKIPNWFLREKGALSRDVFLREHTISIPKLNSALVIKNISETENIFQI
                     GNYSYGINTKQIDLQDILTNPFYWLGNVKLVEFAGPGIFRWYRKTEREKDIDFIERIE
                     SVKNDDQTRNSFLEILNANEISILGLHFAMIMLQSELIDDNNKAVKYFSEILKDSKST
                     HYKLAVEIAFRPKLIKEISIRRKMFLAAFQTLRKDNFRKLLEIYTAADCKFLNDEIIT
                     TIDNISRGDENLQEIESVIENLIASFPPETHLSNTSIPNLFELLVSYGVNHPSSYERI
                     RRFFLQYELYGSTEEIRIAALKSRTEIRNQFTMWLGQNQRVAIDPETGEEYRWDDVLI
                     FDQAISEEDQLIIRKAINEKQIIREAIFLFSGHVLISLNNILPSGVWISKYSESENRS
                     VFRVTVQTRFQGGFDLAIHLNHNEPAEMIEEEIKWKVIAGTEVNGEKLAAKLGGLWEN
                     YNLWTEEFVGVESVERFIRREYKRNDELTIEKLRNLWKFFVWNATSAYVKFWKLSDMK
                     MELTDTTPDGLIISPHDYQTGCIITSFSKRRKTESTLAFIMNFYESFVKQTEEKYPLI
                     KKASVWNAIFSGIIEAEGVDNGINLINKFRRELGNSDIQKKEDISTRIDSFIRSVKNN
                     GYLPKQLYFAVKRFHRWYNLNRSASLSAQSEMIYELYETYRLFDLEEQYPAARTRFFL
                     ETVFYNSTQRFKDVLRELVRKQRHRKISKEESLKLINALHFEFELDEKETYFITRLGY
                     PHLKPSDSAALLTIKSNITAQPNLVVQLQDDDGNIYTIRNPINPKEISKLHQLYLEAN
                     LNVNFRPEHQFLVAVSERGFIIGGTFYYRSDEDAVHMEKIVVSNRYRRKGISEGLMNE
                     LFSRVKSENIKLVTTGFFRPEYFYRFGFKIERKYSGLVKEL"
     gene            6381..7112
                     /locus_tag="VF870_07300"
     CDS             6381..7112
                     /locus_tag="VF870_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9252031.1"
                     /translation="MSREIHRWYSPNLNKEMEIVVYGHYGYALLMFPTAGADYLEYER
                     FKLIDSIGGYLHDGKLKAFSINSINNESWLNNHMHPAHKAIRHQQYNRYVVEEVVPFI
                     HNHSKGLVPIITTGASLGALHAANNFFRRPDIFSGTIAMSGSYDLKSYTKGYFDDNVY
                     FNSPVDYLPRWDDNFMFDHMRRGRIIIASGQGNFEDPEASKQLSNILNVRGVKHWLDL
                     WGHDQPHDWPTWRSMLPYFLGHIKF"
     gene            7252..7866
                     /locus_tag="VF870_07305"
     CDS             7252..7866
                     /locus_tag="VF870_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HEX9252032.1"
                     /translation="MSWKEFLKEKRNRTEVLITIILLSAVLTTLTNFLNYVEARKGVV
                     LPDPVLNMFNPIDLTWLTFALIYVSIFVAIVTLSKNPRRLLFAVQVYTLMVAVRIVAM
                     FLLPLNPPAKMIILNDPFVEFFGTGQTLTKDLFFSGHTATLLILFLVSENKIIKTVFL
                     ISTIAVAISVIMQHVHYSIDVFAAVFFAYACYKLVKKIKTNFSL"
     gene            7863..8204
                     /locus_tag="VF870_07310"
     CDS             7863..8204
                     /locus_tag="VF870_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252033.1"
                     /translation="MNEELNKEEIFSIQKFVSKDFVKSDYSSLIPSNDFEKLEEFRKY
                     LTEKMKEMLDKNYNLLINTLYRIDISEKKLAELFSSKNKESIPPKLADLIIERQIEKI
                     NFRRRYREGKL"
     gene            8361..>10859
                     /locus_tag="VF870_07315"
     CDS             8361..>10859
                     /locus_tag="VF870_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252034.1"
                     /translation="MFENKRLRELEKENEELLFKINGIAEKEDNVRNLNDILKKMRME
                     VADLNERKLSLSAEIESLREQKRQTKADIEGFNKEILNLRHLKLEEQNILLDYSSRID
                     RIKNQISNDENLKDLDLNSDNDLIISRNKEIEALNKEYDELNDKMFAAEEKIDELNSL
                     EDELNERIIEKKKELESLNNIKLKKSYKDVSNVEDKISELTDEQHRVLNEIHEKEKEI
                     TELNKKLFDLKEKENNSVNLIKELDEEVKEKTKQADFLGSKISDLYHEEETRKKQIRE
                     LVRQADEQKQKVEAIQSRISTLEEEENKKKSLLSKVFDQLKFKENLAEDKKQNLEDLD
                     KIGKERIHLIEELSREAKEQELRLEVINSEIARLSEEEISKTKKVEELNELIQTNQAQ
                     LDEKKNNVKELELLEQQRKNNVDKLSDDVILKTQEVEELNELIKVNVTQLDQKRNEFK
                     ELESLEQERNEQFAKLSQELELRTKKVEELNELINVNEAQLNRKRIEFRELELMEQER
                     IEKIDKLSKDIDLRGTKSEELKKELSVLIDEIENKKAIVLNLSDQIKQDETYHEEKVL
                     LNKNLDSEEREKVFRIEELNIEIEEKEQKVELIRENLSQLAKTEEQTKAELEKLVDKL
                     NVTESLFIEEIIPAKSELEKVTQEINNKTERLTELTNQLKNEESLLEQQKLAVNELDS
                     KQRLYMERLEKLEDEINDQEQKCDSLKSVIAQLTEEEDERRNRLDELSLALNTNQEQI
                     DKRVIRLSALEKDEEEIKLRIENLTEKLKEEEQKSEILKTEVEELTNENIQKKIKLEE
                     ISAEIDEKTASVTNRIIALENSENEKKIALEELTN"
BASE COUNT         4013 a         1614 c         2009 g         3223 t
ORIGIN      
        1 agaatataat acaggcaagc ttaaaaagaa tgattactgg gctccaactt atgaagatgg
       61 tttaaatctt cttgcaaaac ttcccggtat agctgcattc atctatagat taaaatataa
      121 gaatggtgaa ttaatagact cagatcctaa acttgatctt ggcggtaact ttgctcatat
      181 gatgggcatt gcaaaacctt acgatgatgt tgcaagaatg tattttattc ttcacagcga
      241 tcacgaaagc ggtaacgtta gcgcacatac aggacatctt gttgcaagtg cattatcaga
      301 tctttactat tctatttctg caatgacaaa cggacttgcc ggaccactac acggattagc
      361 aaatcaggaa gtgcttgcat ggctgcaagg tgtttatgat aaaatgggcg gaaaagttcc
      421 taccgaagaa gagatgagac aatttgtttg ggatacttta aaaggcggac aggtaattcc
      481 cggatttggt catgctgtat taagaaaaac agatccacgt tatatggcac agagagaatt
      541 ttgcttaaag catttaccaa atgatccatt gtttaagtat gtagatctac ttttcaaagt
      601 tgttcctcca gttctgttag aacaaggtaa agcaaaaaat ccctggccaa atgttgatgc
      661 tcaatcagga gttatacaat ggtattatgg attaaaagaa tatgatttct atacagtttt
      721 atttgctgtt ggcagagcac ttggtgttgt tgcaaatatt atttgggatc gcggtcttgg
      781 ttatccaatc gaaagaccca aatccgttac aactgcaatg ctcgaagaag ctgcagggat
      841 aaagaactga ttaatattct tattccggat caagtccgga atatttttct tttaataaac
      901 gccaccgaat agcccaataa accgggaaat aattctgtgg cgttttattt ttaaatagag
      961 aaacaattaa tatggttgat gataaagaaa gacagaacag taatcagatt aaaatgatga
     1021 gcataagaat gggtctttga gtagatttgg gtattgcaat tggggcagca atgcacaaag
     1081 tagcaatagg tcttgttata ggtattatca gcgggggaat aggcataagt atagatcgct
     1141 tgcttaaaag aaagagctaa ttgtttttat ctaacatttt atccaaatta gaggtaaatg
     1201 tttctctgag ttttttatta atgaccaact attaaaattc actccaataa attattatat
     1261 tcccaaaaac aatttttcat tctcttatta aagctttaaa aatgactaac gagccggttt
     1321 catatttaac ggaaataaat gacaaacttc ttgcagggaa agcatcttta gaacaaaacg
     1381 agattgatga tttacttgtt aaccttcgta tagaattgaa tcgcgcaata agtaaaaaac
     1441 aacttggttc agatacagat accggggcct ggcagaatat atttgatggc ctattcagca
     1501 gtgaacttgc aaatcctttt atggaaaata tggataagga tttgttcttc tcttttggtg
     1561 aatatcttgc ttccctttca aatgagccaa gcgaaaggaa tgtttcaatc acccataatt
     1621 atctcaatct ttttcgcaat tcatctttcc ttcataaaat atatgatgaa agaagatggg
     1681 ataatttaat ccattcactc atcgttcaaa gcaattacac atttgatgtt ttatttaatc
     1741 agcgcgtcaa tcagtacaaa aagaaaaatc ttttcagaat aattaaagac agccgcacca
     1801 ttgattatag ctggcaaaaa acttctgaaa tagtttcaac atatcgttca tcaatccatc
     1861 atattctttt tgaaatagag agtgaaagta aaatagttgc gttcttgctt gaaaacagtt
     1921 tagaaatggt tatgctggat cttgcctgct taacatcagg aattgttaac gcgatgattc
     1981 ccgcaaactc tgtttcagaa catatttctt ttatacttaa tcaaaccaaa gcacccatcc
     2041 tttttgctga tgatgaaaaa caacttgcaa aaataagatc cataaaaaaa gaaactccat
     2101 ttcttaaaac cgttgtaatg ctgaaaggta atgctgcaga agattgggta attagttttg
     2161 aagagtttaa agcaatggca tccgatcata gcattaagac agatgtgcag ataaaacctg
     2221 attcgcttgc aactttgatg tatacatcgg gaacaacagg cgaaccaaaa ggaataatgt
     2281 tttcccacac caatattgtt tataaaagat tctgccgcgc aatggcaata cccggcataa
     2341 gtgatgaaga tcgttatctt gcatttcttc cactatacca cacctttggc aggtggcttg
     2401 aaatgacggg cgctattttc tggggagctg aatattgttt tatggaaaat ccttcagttg
     2461 aaacaatgat tgaaaatatg aggcatgtta aaccaagtat ttttatttca attcctaaaa
     2521 aatggatcca gctttatgaa tatataactg ctcgaattga tgttgaagtt gatgatgatg
     2581 aaaaaattaa agaggctgtg gttagtgcaa cgggcggtaa gctaaaatgg ggactttcag
     2641 cagcaggatt tctcccgcct gatattttca gatttcttca gagatatggt attgaactga
     2701 tgagcggttt tggtatgaca gaagcaaccg gaggaataac gatgacccct ccattccagt
     2761 atcgtgaaaa ctcgcttggt aaagcattac ccggcattga aattaaactt ggtgaagatg
     2821 gtgagatatt aatccgcggc ccttatgtaa tgctaaatta ttatggtcaa aaacttgaag
     2881 atacttttga tgccgatgga tggctggcaa ccggggatgt aatgcgaatg gatagagatg
     2941 gttttataga aattattgat cgtaaaaaag agatttataa aaatataaaa ggggaaacca
     3001 tagcgccgca gaaaatcgaa aacttatttc gtgattttga atttgtaaaa caggtctttc
     3061 ttgtcggtga tcatcgtccc tttaatacag ttataatctt tcctgatatt gaatccgaaa
     3121 tctcaccatt aaatcaaatg gatgagcagc agagacagga gtatatttca actttaattg
     3181 taagcgtaaa taattttctt gcaccatttg aacgtatttt ggattttaga attattaccc
     3241 gtgcttttga tgatgctaac ggcgagctaa ctccaaaagg aacttataag cgcagggtta
     3301 tcgaaaagaa ttttgacgat cttatcagat caatgtatat aaaagatcat ctttcgcttg
     3361 tgcttgataa gtacgaaatt aaaattccaa actggttctt gcgtgaaaaa ggcgcattaa
     3421 gtcgtgatgt ttttctaaga gagcacacta tttcaattcc aaagcttaac tcagcattgg
     3481 taattaaaaa cattagtgaa acagaaaata ttttccagat aggaaattat tcgtacggca
     3541 taaacacaaa gcaaattgat ctgcaggata ttttaacaaa tccattttac tggcttggta
     3601 atgttaaatt agttgagttt gccggtcccg gaatattcag atggtataga aaaacagaac
     3661 gtgaaaaaga cattgatttt attgaacgaa ttgaatcagt taagaatgat gatcaaacaa
     3721 gaaatagttt tttagaaata ctaaatgcaa acgaaatttc cattttagga ttacattttg
     3781 caatgatcat gcttcaatca gaactgattg atgataataa caaagccgtt aaatattttt
     3841 cagaaatact taaagattca aaatccactc actacaaact tgctgttgaa atagcattca
     3901 ggccaaaatt aataaaagag atttctatcc ggcgtaaaat gtttttggca gcatttcaga
     3961 ctttaagaaa agacaatttc cgtaagctgt tggaaattta cacagcagca gattgcaagt
     4021 ttctaaatga tgaaataatt accacgatag ataatatcag caggggtgat gaaaatttac
     4081 aggaaataga atcagtaatt gaaaatctta ttgcttcttt tccacctgaa actcatttaa
     4141 gtaatacatc aataccaaat ctctttgaac ttcttgtaag ctatggcgtt aatcatcctt
     4201 cgagctatga aagaatacga aggttctttt tgcaatatga gctttatgga agtaccgaag
     4261 aaattcgcat agcagcatta aaatcccgaa cagaaatcag aaatcaattc actatgtggc
     4321 ttggtcaaaa tcagcgtgtg gcaatagatc ctgaaacagg cgaagaatac cgctgggatg
     4381 atgtattaat ttttgatcag gcaatttcag aagaggatca attaattata agaaaagcga
     4441 taaatgaaaa acaaataatc cgtgaagcta tatttttatt ttccggacac gttttgattt
     4501 cgctcaacaa tattcttcca tccggagttt ggattagcaa atattcagaa tcagaaaaca
     4561 gatctgtttt tcgtgttacg gttcagacca gattccaggg cggatttgat cttgcaattc
     4621 atcttaatca caatgaacct gctgaaatga ttgaagaaga aattaagtgg aaagttatag
     4681 caggtacaga agtaaatggt gaaaaacttg cagctaaact tggcggattg tgggaaaact
     4741 ataatttatg gactgaggaa tttgttggtg ttgaatctgt tgaacggttt atcagacgcg
     4801 agtacaaacg taatgatgag ttaacaatag aaaaactgag aaatctctgg aaattttttg
     4861 tatggaacgc cacctctgct tatgtaaagt tctggaagtt atctgatatg aagatggaac
     4921 ttacggacac aactccggat ggtcttatca tttcacctca cgattatcaa acgggttgta
     4981 taataacttc attttcaaag agaaggaaaa ctgaatcaac acttgctttt ataatgaact
     5041 tttatgaaag ctttgtaaaa caaactgaag aaaaatatcc actgattaaa aaagcatcgg
     5101 tttggaatgc aatattttca ggaataattg aagcagaagg agttgataac ggaataaatc
     5161 tgattaataa attcagaaga gaacttggca actcagatat tcaaaaaaaa gaggatataa
     5221 gcacgcgaat tgattctttt atccgaagcg ttaaaaacaa tggatatctg ccaaagcagc
     5281 tttattttgc ggttaaaaga ttccatcgct ggtataacct taacagaagt gcttctcttt
     5341 ccgcacaatc agaaatgatt tacgaattat atgagacgta tcgtttattt gatcttgaag
     5401 aacaatatcc tgcagcacga acacgatttt ttcttgaaac agttttttat aattcaacac
     5461 aaagatttaa agatgtctta cgtgaactcg taagaaaaca aaggcatcgt aaaatatcca
     5521 aagaggaatc actaaagctt attaacgcat tacattttga atttgagctt gatgaaaagg
     5581 aaacttactt tattacccga ttaggctatc cacacttaaa gccatccgat tcagccgcat
     5641 tgttaacaat aaaatctaat attactgcac aaccaaacct tgttgtgcaa ttgcaggatg
     5701 atgacggtaa tatttatacg ataagaaatc caattaatcc taaagaaatt tctaagttgc
     5761 atcaacttta tcttgaagca aatctaaatg taaatttccg gccggaacat caatttcttg
     5821 ttgcagtttc tgagcggggc tttattattg gcggaacttt ttattaccga agtgatgaag
     5881 atgcagttca tatggaaaaa atagtggtat ccaatcgata tcgccgcaaa ggaatcagcg
     5941 aaggattaat gaatgaattg ttcagcagag ttaaatctga gaatataaaa cttgttacca
     6001 ccggtttctt tcggccagag tacttttaca ggtttggctt taaaatagag agaaaatact
     6061 cgggtttggt taaagaactt tgatgaagtc taacacgctt tgaagtttta ttccgcttat
     6121 aaaacaaaaa tggattattt aattcaggtg aactccatat aaaaaaagtc ccagatgtat
     6181 aggtaatcat cggggtagta cagataatgt ttatgtttct catcaaaata ttttagcatc
     6241 attatgaatg ctgattaact atgttcagtt gctgttttta actttgtaca atacttaaaa
     6301 gatcgcctta ccacctattt ccaaatttta attcatttct ttactttcac acttataatt
     6361 tcaatctata aggagtttta atgagcagag agattcaccg ctggtacagc cccaatttaa
     6421 ataaggaaat ggagattgtt gtttacggac attatggcta tgctttatta atgtttccta
     6481 cagcaggagc agattatctt gagtatgaac gattcaaact tatagattca atcggcggtt
     6541 atttgcatga tggtaaactt aaagcttttt cgattaatag cataaataat gaaagctggc
     6601 ttaataatca tatgcatccg gctcataagg caattcgtca tcaacaatac aatagatacg
     6661 tagttgaaga agttgtgcct tttattcata atcatagcaa aggattggtc ccgattataa
     6721 caacaggcgc ttctctcggt gcacttcacg ctgcaaataa ttttttcaga cggccggata
     6781 ttttttcggg taccatagca atgagcggca gttatgattt aaaaagttac acaaaaggat
     6841 attttgacga taacgtttat tttaattctc cggtagatta tttgcctcgt tgggatgata
     6901 attttatgtt cgatcatatg agaagaggca gaattatcat tgcatcgggt cagggaaatt
     6961 ttgaggaccc cgaagcgtca aaacaattat caaatattct aaatgtccgc ggagttaagc
     7021 attggttaga tttgtggggc cacgatcaac cccatgattg gccaacatgg agaagtatgc
     7081 ttccttattt tttaggacat ataaagtttt agtatagatg tcatctcgaa tccactttag
     7141 tggatgagaa atctttgcta ttaatagaag aagagttatt gttgtaaagc ttctggaaat
     7201 gaccctcgaa gttttgtagg aaaataataa aacatttatt tacatgggcg tatgagctgg
     7261 aaagaatttt taaaagaaaa aagaaacagg actgaagttt taataacgat aatattgtta
     7321 tcagcagtat taacgacttt aacaaatttc ttaaactatg tagaagcccg taaaggtgtt
     7381 gtattaccgg atccggtttt aaatatgttt aatccaatag atttgacgtg gcttactttt
     7441 gcactaatat atgtgtcaat ttttgttgca atcgttacgc tttctaaaaa ccccaggcga
     7501 ttgttgtttg ctgttcaagt atacacttta atggttgcgg taaggatagt ggcgatgttt
     7561 ttacttccgt taaatccacc ggctaaaatg attattctta atgacccatt tgtggaattt
     7621 tttgggacgg gacaaacatt aaccaaagat ctttttttct caggacatac tgcaactctg
     7681 ttaattttgt ttctagtttc tgaaaataaa ataattaaaa ctgttttcct gattagtaca
     7741 atcgcagttg caatttctgt tattatgcag catgtacatt attcaataga tgtttttgcc
     7801 gccgtgtttt ttgcctatgc atgttataaa ttggttaaaa aaattaaaac taatttttct
     7861 ttatgaatga agaattaaat aaagaagaaa tattttctat tcaaaaattt gtttcaaaag
     7921 attttgttaa gagcgattat tcatctctta ttccaagtaa tgattttgaa aaacttgaag
     7981 aattcagaaa atatctcact gaaaaaatga aagagatgct tgataaaaat tataacttat
     8041 taattaacac actttacaga atagacataa gtgaaaaaaa attagcagag ctattttcat
     8101 caaagaacaa ggaatcgatt ccaccaaaac tagcagacct tattattgaa agacagatcg
     8161 aaaaaataaa ctttcgcaga cgttaccgtg aaggaaaact ttaatcattt tttcaaccaa
     8221 atccgcaagt aagatttaca tggtgtaaaa ttttgtttaa ttgaaatttt gccacgcaat
     8281 acctaatttc atcttgaata tttatacaat tatctaattt tttgataata taggcatact
     8341 taataggagc cattatgggt ttgtttgaga ataaaagact acgagaatta gagaaagaaa
     8401 acgaagaact tctctttaaa attaatggta ttgccgagaa ggaagataac gttcggaatc
     8461 ttaatgatat cttgaaaaag atgagaatgg aagttgctga tttaaacgaa cgcaagctct
     8521 ctttatctgc cgaaatcgaa tcattacgcg aacaaaaaag acaaactaaa gccgatatag
     8581 aaggattcaa taaagaaatc cttaacctgc gtcacttaaa actagaagaa caaaacatcc
     8641 tgcttgatta ttcttccaga atcgatcgga ttaaaaatca aataagtaat gatgaaaact
     8701 taaaagattt agatttgaat tctgataatg atttaatcat tagcagaaat aaggaaattg
     8761 aggctcttaa taaagagtat gatgaactca atgacaaaat gtttgccgcg gaagaaaaaa
     8821 tagacgaact aaattcgctt gaagatgaat taaacgaaag aataattgaa aagaaaaaag
     8881 aacttgaatc gttgaataac ataaaactta aaaaatctta taaagatgtt agcaatgttg
     8941 aagataaaat cagcgaatta acagatgagc agcacagagt actcaatgaa attcatgaga
     9001 aggaaaaaga aataacagag ttgaacaaga aattatttga tttgaaggaa aaggaaaaca
     9061 attcggttaa tcttataaaa gaactggatg aagaagttaa agaaaaaact aaacaagctg
     9121 attttcttgg atcgaaaatt tcagatctct atcacgaaga agaaacgcgg aaaaaacaaa
     9181 taagagaatt agtcagacag gctgatgaac aaaagcaaaa ggttgaggca atacaatcaa
     9241 gaattagcac ccttgaagaa gaagaaaata aaaagaaatc acttctctca aaagtttttg
     9301 atcaacttaa gttcaaagaa aatctggctg aagataaaaa acagaatctt gaggatcttg
     9361 ataaaattgg taaagaaaga attcatttga tcgaggaatt atcacgtgaa gcaaaagagc
     9421 aggaattaag gcttgaagta atcaattcgg agattgcaag actatctgaa gaagaaatat
     9481 caaagactaa aaaagttgaa gagcttaatg aacttattca aactaaccaa gctcaattag
     9541 atgaaaagaa aaataacgtt aaagaacttg agctattgga acaacaaaga aaaaataatg
     9601 ttgataagct ttctgatgat gtaattttaa aaactcaaga agtagaagaa cttaatgaac
     9661 ttattaaggt taacgtaacc caattagacc aaaaacgaaa tgagttcaaa gaactcgagt
     9721 cgttggaaca ggaaagaaac gagcagtttg ctaaactatc ccaggagtta gaattaagga
     9781 ctaaaaaagt tgaggaactg aatgaactta tcaatgttaa cgaagctcag ttgaacagaa
     9841 agcgaataga gttcagagaa cttgagttga tggagcagga aagaattgag aagattgaca
     9901 aattatctaa agatattgat ttacgcggaa caaaatcaga ggagttaaaa aaagaacttt
     9961 cagttttaat tgatgaaata gaaaataaaa aagccatagt attaaattta tcagatcaga
    10021 ttaaacagga tgaaacttac catgaagaaa aagttttact gaataaaaac cttgattctg
    10081 aagaacgaga aaaggttttt agaatagaag aacttaatat tgaaattgaa gagaaagaac
    10141 aaaaggtcga attaatcagg gaaaatttat cacaacttgc aaagacagaa gaacaaacaa
    10201 aggccgagtt agaaaaatta gtagataaat tgaatgttac agaatctttg tttattgagg
    10261 aaattattcc tgcaaaatca gaattagaaa aggttaccca ggaaattaat aataagactg
    10321 aaagattaac cgagttaaca aatcagttaa agaatgaaga atcattatta gagcagcaaa
    10381 aacttgctgt taatgaactg gattccaagc aaagattata tatggaaagg cttgagaaac
    10441 tggaagatga aataaatgat caggaacaga aatgtgattc attaaaatca gttattgcac
    10501 aactaactga agaagaggac gagagaagaa accgattaga tgaattatcc ctcgcattga
    10561 atacaaatca ggaacagatt gataaaagag taattagatt atcagcgctt gaaaaagatg
    10621 aagaggaaat aaaacttaga attgaaaatc tgacagaaaa gcttaaagaa gaagaacaaa
    10681 aatctgaaat tctaaaaact gaagttgaag aacttacaaa tgaaaacatt cagaaaaaga
    10741 taaagcttga agaaataagt gcagaaatag atgaaaagac agccagcgta acaaatagaa
    10801 taatagctct tgaaaacagt gagaacgaga aaaaaattgc tttagaagaa cttactaat
//
LOCUS       DASWBY010000407         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_19580, whole genome shotgun sequence.
ACCESSION   DASWBY010000407 DASWBY010000000
VERSION     DASWBY010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19580"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VF870_07320"
     CDS             <1..399
                     /locus_tag="VF870_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252035.1"
                     /translation="YIDYFGNVITIGKKNRVFQMPLNFGVQLRLFENSIADNLRPFVC
                     AAAGPTVLITTPYQEEFFNSFAYAQTEYAVGGYVGLGANFGLDKSSLVGITFKYYYSK
                     ILTGGVESLYGREKTEIQGFFITLNLGMMY"
     gene            complement(402..941)
                     /locus_tag="VF870_07325"
     CDS             complement(402..941)
                     /locus_tag="VF870_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9252036.1"
                     /translation="MSDDKLKIDSSIPNFSLTGVDDKTYSLNDFSDKKILVVIFSCNH
                     CPYVHAYEDRIMELQKEFEKDGVQIVAINSNDDVKYPDDSFAEMKKRATARGFNFPYL
                     RDKSQDIAKAFGATHTPQIFLFNSDRKLKYEGKIDDNWQEPDKVKSQYLKDAILELLN
                     ENEVSVPETFSIGCTIKWK"
     gene            999..1589
                     /locus_tag="VF870_07330"
     CDS             999..1589
                     /locus_tag="VF870_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9252037.1"
                     /translation="MKTLLLLIIIISETIMSQNYDFEQHLYQSYDRYKENSLTNRRFK
                     HSDITLLIDKLKHKSGFTVKVVGKSVEGRELKLITIGHGKKKIFMWSQMHGDEPTATA
                     ALFDIFNFLSDTSNKAFCKELFDRVTLYFLPMVNPDGAERFKRRNFYDIDLNRDASRL
                     QCPEAVILKNVFDSLKAEFGFNLHDQSHRYSVGNSF"
     gene            1638..>2149
                     /locus_tag="VF870_07335"
     CDS             1638..>2149
                     /locus_tag="VF870_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252038.1"
                     /translation="MNDVRGKAVKLIGNMYQALSQFIPGHIAKYSDEYEPRAFGDNFQ
                     KWGTSTILIESGGWKNDPEKHFIRKINFIALLSAFKSIAEKNYVNTSSEIYESIPFND
                     KYIFDVLLRNLTIKSGKEKIKIDIGINSDEFEGPNEKKIFYKSQVDDLGDLSTFYAYN
                     DYDFEGYEIER"
BASE COUNT          715 a          340 c          377 g          717 t
ORIGIN      
        1 tatatagatt attttggtaa tgtaattaca attggaaaaa agaacagagt ttttcagatg
       61 cctctaaatt ttggtgtgca acttagactt tttgaaaatt ctattgccga taatttaaga
      121 ccttttgttt gtgccgcagc aggtccaaca gtgcttatta ctacgccata tcaggaagaa
      181 ttttttaact cctttgctta tgcgcaaacc gaatatgcgg ttggaggata tgttggtctt
      241 ggtgcaaatt ttggcttgga taagtcaagc ttagttggga ttacctttaa gtattactac
      301 tctaagattc ttactggcgg agtagaaagt ctttacggaa gagaaaaaac tgaaatacaa
      361 ggttttttta ttacgcttaa tttgggaatg atgtattagt atcacttcca cttaattgta
      421 cagccaattg aaaatgtttc tggtactgaa acttcatttt catttagtaa ttctaaaatt
      481 gcatctttta aatactgaga tttaactttg tcaggttcct gccagttatc atcaattttg
      541 ccttcgtact ttaattttct atcactgtta aaaagaaaaa tttgcggtgt gtgtgttgct
      601 ccaaatgctt ttgcaatatc ctggctttta tctcttaaat acggaaagtt aaatcccctt
      661 gcagttgccc gcttcttcat ctcagcaaat gagtcatcag gatactttac atcatcgttg
      721 gagtttattg caactatctg cacgccgtct ttttcaaatt ctttttgcaa ttccattatt
      781 ctgtcttcat atgcatgtac atacggacag tgattgcagc taaagattac tactaatatt
      841 ttcttgtcag aaaaatcatt taagctgtag gttttatcat caactcccgt taaagaaaag
      901 ttgggaattg aagaatctat tttcaatttg tcatcagaca tagtaaattt tcctttagtt
      961 aaataatctt ttagttaact gaacaaaaat atcaaaaaat gaaaacactt ctcttactca
     1021 taataataat ctcagaaacc attatgtcac aaaattatga ttttgaacag cacctgtatc
     1081 aatcttatga tcgatataag gaaaatagtt taacaaacag aagatttaaa cactctgata
     1141 taactctatt aattgataag cttaaacata aaagcggatt tacagttaaa gttgtgggta
     1201 aatctgtaga gggcagagaa ttaaaactaa ttacaatcgg tcacggcaaa aagaaaatat
     1261 ttatgtggtc acaaatgcac ggggatgaac caactgccac tgcagcgtta tttgatattt
     1321 ttaatttcct ttccgataca tcgaacaaag cattttgcaa agaattgttt gatagagtaa
     1381 cgctatactt tttaccaatg gtaaatcccg atggagcaga aagatttaag cgaagaaatt
     1441 tttatgatat tgatttaaat cgtgatgcat ctcgtttgca gtgccctgaa gctgtaattc
     1501 taaaaaacgt atttgatagt ctgaaagctg aattcggatt taacctgcac gatcaaagtc
     1561 atagatattc agtcggcaac agtttttgaa ctgccacaat aagttttctt gcacccgctt
     1621 ataattatga aaaggatgtg aatgatgttc gcggcaaggc agtaaagtta attggaaata
     1681 tgtatcaggc tttatcacaa ttcattcccg gccacatcgc aaaatactcc gatgaatatg
     1741 aaccaagagc atttggtgat aattttcaga aatggggaac gagtacaatc ctgattgaat
     1801 cgggcggatg gaaaaatgat ccggaaaaac attttataag aaaaattaat tttatcgctt
     1861 tgctttctgc attcaaatca attgcagaga aaaattatgt aaacaccagc agtgagattt
     1921 atgaatcaat tccttttaat gataaataca tttttgatgt gttgctaaga aatctgacaa
     1981 taaaaagcgg aaaagaaaaa attaagattg atatcggaat aaactcggac gaatttgaag
     2041 gtcccaacga gaagaagatt ttttacaaat cccaggtaga tgatcttggt gatctttcaa
     2101 ccttctatgc atataatgat tatgattttg agggttatga aattgagcg
//
LOCUS       DASWBY010000408         3602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_504957, whole genome shotgun sequence.
ACCESSION   DASWBY010000408 DASWBY010000000
VERSION     DASWBY010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504957"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..734)
                     /locus_tag="VF870_07340"
     CDS             complement(237..734)
                     /locus_tag="VF870_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252039.1"
                     /translation="MRLFQTILFYAILITFFIHTEGHSQSVQAEIKNLSEGADMIVTG
                     KVVNQKSEWSSDKSKIYTNVTIQVDEFLKGSNNQNQIVIKNLGGEVGNVGETYSHVPT
                     FANEEDVLVFIKKSEKDQSLRVFQGDAGKMTFYQDKKTGEKVTSRNIKASEMKKEIKN
                     YVEKQ"
     gene            1255..>3602
                     /locus_tag="VF870_07345"
     CDS             1255..>3602
                     /locus_tag="VF870_07345"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX9252040.1"
                     /translation="MSLNSDNHFTRILKIDFYRIVLTILSLVLLISFPVFPQNENIRF
                     KHLSSDDGLSQNFVSCILQDQKGFMWFGTKDGLNRYDGHTFVVYQHDPFDTSSISDNF
                     ITSLFEDSRGYIWVGTLTGGLNVFDRKTETFHQINYTPNASKVTVTEEVKAIVEDSKG
                     NIWIGTRGEGVIKITFNKKNSFDITYKKFVSQSGKPGSLSNNIVSTLYHDSKGVLWVS
                     TLTGLDKFDYQTESFTHYEIQTRNPKAPPNYFDKTIYSMLESKNGNLWLGTLSGLVKF
                     DRQTGSYKLYPHHYDIFRYGWGSVMQIVEDNSENLWLATPGELMRFNPKSKSYDYFKN
                     DYYDQSTLSYNSISSLFIDRTEILWVGTSGMGINYYDPKVNRFSTLFIKSNPSSRVTG
                     FSVRSVFQENEDIIWIGTEVLLKWNRRTGAIKSYETDSNRPDDFGNTGPWSIIKSHDG
                     KLWMATIQGVYSYDPSTDKSKQYKFNPNDTLGLPQKEAYAVFEDHQKNIWVATENFFC
                     RLVDSDKGIFQSYRYQTPLPYNQRVRPVIHQDIKGIFWLGTRNGLLKFNLQNKSFYDY
                     KNDPSKPNSLNNDNINSICPDPLNPDKVLWLGTSGGGLNRFNIDEETFEHFTEKEGLP
                     NNVIYGILTDGKGNLWLSTNKGLSKFNPKEKTFRNYDVNDGLQSNEFNTGAYFKNING
                     EMFFGGIKGLNYFYPDNIKDNPFIPNIVITDIKLGDHYLSNKNDNSFLSKSISETDEL
                     VLSYAEDVITFEFASLDYSAPGKNRYAYKLENFNTDWIYSGSV"
BASE COUNT         1257 a          548 c          584 g         1213 t
ORIGIN      
        1 atctgagtta gcggcaccgg atggagaagg accatcgttt atataaacct gactggttgt
       61 tccgttaaaa agaaatgcat tattatttcc accttgagca aatgcagcat ttgaacttaa
      121 agtgatagaa agcagaacag ctataaagaa ataaatatta tttaaaaatt tcatattatc
      181 ctcaatttta ttgttattaa aaaccgttga aattaattga tttattgaat cagtcattac
      241 tgtttctcaa catagttttt aatttccttt ttcatctcac ttgctttaat atttcttgaa
      301 gttacttttt caccggtttt tttatcctga taaaaagtca ttttgcctgc atcaccctgg
      361 aatactctta aactttgatc tttctctgat ttttttataa aaaccaaaac atcctcttca
      421 ttagcaaatg tcggtacgtg gctgtatgtt tctcctacat ttccgacttc tccaccaaga
      481 tttttaatta ctatctggtt ttggttgttg gatcctttta aaaactcatc aacctgaata
      541 gtaacattcg tgtagatttt ggatttatca gaactccatt ctgatttttg attgactact
      601 ttaccggtaa caatcatatc ggctccttct gaaagattct tgatttcagc ctgaacagat
      661 tgtgaatgcc cttcggtatg aatgaaaaat gtgattagaa tagcataaaa aagtattgtt
      721 tgaaataatc tcatagcata attctcctgt aaattctgaa aatagtttta atcgcgtgca
      781 aaaataaact tttaagtctc tcatgtgtta ggacaattaa agcaaataat tggactttta
      841 ttgcattttg aaaaaatcac aaagatttcg tttttggatc gtaataaacg aaaatatatt
      901 ttttttaaat cggatttgat ataatgtagt tataataatt aatatctaca attatggata
      961 ttatcaaatg taaatagtta gaattatata aactaagaat attattgctg cgcattggat
     1021 acctggtgag ccagatagct ttaaatcttt aattcggtgt aagaaaatct taacaataaa
     1081 actttttaat aatagattaa atactgtatt ttttaggcag cattattttg agtaaaatca
     1141 tattgtcctt tttgaaaaac tctcaagctt aaatttttag atttttaaaa tgggatgtaa
     1201 aaattaaaaa tatctattta gatcaaatta aataagttaa aacattaaca actgatgtca
     1261 ttaaattcag ataatcattt cactagaata ctaaagattg atttttatag aattgtttta
     1321 actattctat cattagttct gttgatttcc ttccctgttt tcccgcaaaa tgaaaatata
     1381 agattcaaac acctttccag cgatgatgga ctttcacaaa attttgtttc ctgtattctg
     1441 caggaccaaa aaggctttat gtggtttggt accaaagatg gattaaacag atacgatggt
     1501 cacacatttg tagtttacca gcacgatcct tttgatactt caagcatttc agacaatttt
     1561 ataacttcat tattcgaaga cagtcgcggc tatatatggg ttgggacttt aaccggagga
     1621 ttaaatgtat tcgatagaaa gactgaaact ttccatcaga ttaattatac tccaaacgct
     1681 tctaaagtaa ccgttacaga ggaagttaag gctatagtag aagactcgaa aggaaacatc
     1741 tggattggaa ccaggggtga aggagtaatt aaaattactt ttaataaaaa gaattctttt
     1801 gatataactt ataaaaaatt tgtatctcaa tcaggtaaac ccggcagttt aagcaataac
     1861 attgtttcaa ctttatatca tgattctaaa ggagttttgt gggttagtac tttaacagga
     1921 ctggataaat ttgattatca aactgaaagt tttacgcatt atgaaatcca gactagaaac
     1981 cctaaagcac cgcccaatta ttttgataaa actatttact ctatgcttga aagcaaaaac
     2041 ggaaacttat ggctgggtac gttaagcgga ttagtaaaat ttgacagaca aaccggaagc
     2101 tataaattat atccgcacca ctatgatatt ttcagatatg gttggggtag tgtaatgcag
     2161 attgttgaag ataattccga aaatctgtgg cttgcaactc cgggtgaatt gatgagattt
     2221 aatcctaaat ctaaatcata cgactacttt aaaaatgatt attatgatca atcgacatta
     2281 agctataaca gcatatcaag tttatttata gatagaacag aaatactttg ggtaggtaca
     2341 tcgggtatgg gaattaatta ttatgaccct aaagtaaata gattttcaac tctttttatt
     2401 aaaagtaacc cttcatcccg tgttaccggt tttagtgtgc ggtcagtttt tcaagaaaac
     2461 gaagatataa tttggattgg caccgaggta ttgttaaaat ggaatcgcag aacaggagca
     2521 ataaaaagtt acgaaactga ttccaacaga cctgatgatt ttggaaacac gggtccctgg
     2581 tcaatcataa aatcacacga tggtaaatta tggatggcaa ctatccaggg tgtttacagc
     2641 tatgatccgt caaccgataa atctaaacag tataaattta acccaaatga tactttaggt
     2701 ttaccgcaga aagaagcgta tgctgttttt gaagatcacc aaaaaaatat atgggtcgcc
     2761 actgaaaatt tcttttgtcg gctcgttgat agtgataaag gtatttttca atcctatcgt
     2821 taccagactc cccttcctta taatcaaaga gtaagaccgg taattcatca ggatatcaaa
     2881 ggaatatttt ggctcggaac cagaaatggg ttactaaaat tcaatttaca aaataagtca
     2941 ttttatgatt ataaaaatga tccatccaaa ccaaacagtt tgaacaatga taatatcaat
     3001 tcaatttgtc ctgatccatt aaatccggat aaggttcttt ggttaggtac ttcgggaggc
     3061 gggttaaatc gttttaatat agatgaagaa acatttgaac attttactga aaaagaaggt
     3121 ttacccaaca atgtaattta tggaatacta actgatggaa aaggaaattt atggttaagt
     3181 acaaacaagg gtttatctaa atttaatcca aaagaaaaaa catttagaaa ttatgacgtt
     3241 aatgatggtc tgcagagtaa tgaatttaac acaggagcat actttaagaa cataaatgga
     3301 gaaatgtttt ttgggggaat caaaggatta aactatttct acccagataa tataaaagac
     3361 aatccattca ttcctaatat tgttattaca gatataaaac ttggagatca ttatctgtct
     3421 aataaaaatg ataattcttt cttatcaaaa tcaatttcag agactgatga acttgtcctc
     3481 tcttatgctg aagatgttat aacatttgaa tttgcctctc ttgattactc ggcaccaggc
     3541 aagaaccgtt atgcatacaa gcttgaaaat tttaatacag actggatcta ttcaggttca
     3601 gt
//
LOCUS       DASWBY010000409         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_694744, whole genome shotgun sequence.
ACCESSION   DASWBY010000409 DASWBY010000000
VERSION     DASWBY010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694744"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            499..1287
                     /locus_tag="VF870_07350"
     CDS             499..1287
                     /locus_tag="VF870_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252041.1"
                     /translation="MTKDFVITDRSLEYGPPPENTDRDNIESRQKICALENFPAYFLQ
                     YVFIIEELVGFRLAVIHQDEVKTNEFFPTLKAAKKAFADKYNKKAWEKSVIPRWTDLY
                     APDNDWLIDKQILLKNDMKRVIIEQDRRKAAQRKFRIRIGKMSKLLQTFFLYNAREFG
                     LEYFTVVKSSERIFFVCITKEPKPIMEKVQSIDEALDYFNEHFGKRAKPPVNIIQQWL
                     WLNFERKQEVQADRSGPDGAVSVESTSLKEAFGKFSDFTEVVFL"
     gene            1363..1782
                     /locus_tag="VF870_07355"
     CDS             1363..1782
                     /locus_tag="VF870_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252042.1"
                     /translation="MKTQRNLQERVVGYILTRRDNELKNLTIYELTKKIGASPGAVAR
                     MFRENLNTTFKSYKNQEIATRISLLLKKQPYILMKTVARRFAFQDEADLEKVFEDYFA
                     ISLSSYQACIKNMKRRSLKSLQGIPGFPAGEFEMARN"
BASE COUNT          788 a          423 c          480 g          593 t
ORIGIN      
        1 ttatatgaac cgaaccacca atggcttatc gataagcttg aatttttaaa gaactatact
       61 ccctaccggc aaaataataa acggcggaaa cgcccaataa aaagacagca tgaggtactt
      121 atatatgcct gaaaaattgg ggaacatacg agaaactctc attaagagaa acagcactcc
      181 tcaaacgcat gagaacagga cgcatgagtt tcctcctata tgtgatttta gtattgtcaa
      241 cggttcccag agaaaatcga cagttaatga ttctttctgt catcaagttt tcaacgtcat
      301 ggtttccatg gagacatggt tacacctcct tgcttttaaa tttttgactg ttgcccaaca
      361 aatggtcggt cggaaggaga tgctggaatc agcaactcct tccacccacc tatttccacc
      421 ctacatcaaa actaaagaaa aactaatcga atgttcatta attctgcatc cacagatttt
      481 gaaaaataga ggtgacctgt gacgaaggat tttgtaatta cagatagaag ccttgaatat
      541 ggtccgccgc cggaaaatac agatagagac aatattgaaa gccgacaaaa aatatgcgcc
      601 ctggaaaact tcccggcata cttcttacaa tatgtgttca tcatagaaga gttagtcggc
      661 tttagattgg cagtaattca tcaggacgag gtaaaaacca atgaattctt ccctactctg
      721 aaagctgcca aaaaagcgtt tgccgacaaa tacaacaaga aagcctggga aaaaagcgtc
      781 atcccaagat ggactgattt atatgctcct gacaacgatt ggcttatcga taaacagata
      841 ctcttaaaaa acgatatgaa gagagtcata atagaacaag accgaaggaa agccgcgcaa
      901 agaaaattca ggataaggat tggaaaaatg tctaaattat tgcagacctt ttttttatat
      961 aatgccaggg aattcggcct ggaatatttt acggtggtaa aatcgtcaga aagaatcttt
     1021 tttgtgtgta taacaaaaga gcccaaacct ataatggaaa aagtgcaaag catagatgaa
     1081 gcgctcgatt attttaacga gcactttggt aaaagggcga aaccacctgt aaatataatc
     1141 caacaatggc tctggctgaa ctttgaaaga aaacaggaag tgcaagcaga tcgaagcggc
     1201 cctgatgggg cagtttcagt agaatcgacc agtttgaagg aggcattcgg gaaattctca
     1261 gatttcacag aagtagtttt tttataaaaa gttttttcaa gccattaaca tcaggttggc
     1321 tgcagacgca aaagagcgat caataaaagg agtaaataac ttatgaagac acaaagaaat
     1381 cttcaagagc gagtggttgg ttatatcctg acccgccggg ataacgagtt gaaaaacctg
     1441 actatatatg aattaacaaa aaagataggc gcgagtcccg gagctgttgc ccggatgttc
     1501 agagaaaatt tgaacactac ttttaaatca tataagaacc aggaaatagc cacacgcatc
     1561 tctttgctat tgaaaaaaca accctacatc ttgatgaaga cagtggccag gcgattcgct
     1621 tttcaggatg aagcagacct tgaaaaagtt ttcgaggatt atttcgctat ctctctgtcc
     1681 agttatcaag catgtatcaa aaatatgaaa agacggtcat taaaaagttt gcagggaatt
     1741 ccgggatttc cggcaggcga gtttgaaatg gcgaggaatt agtaggatgc tcccgggagg
     1801 taaattagca caaaaacggg ttcaacatag taacttcatt gtggattgtc ccattcgatc
     1861 cttaaagcag acactccggt tgatttcaac cggtaggtca aagacgtaca aaaaagtcgg
     1921 gcgggtgaag aagtacttcg cagcctcttt acccgaccga ctcttttttt tcatgctatt
     1981 ttcgatagta aaagagggac agcatataac tggacacgca tttatgaatt tctatttaat
     2041 atggcaacgc ttttatttcc ataataaagg gtggggaaaa tattttccct accctcacca
     2101 tttatcgtct aaaacggagg caatatgagt acatggttaa aagaaaaaat atttagcgca
     2161 atcgttagtg taggctggat gatattcatc atcagtagag gtatcaggac aattttaaaa
     2221 ggtagaaaga aggatggtgt gaatttatgg atgtgcattc ataatcgatt tgaacaggag
     2281 gcaa
//
LOCUS       DASWBY010000410         5582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_167237, whole genome shotgun sequence.
ACCESSION   DASWBY010000410 DASWBY010000000
VERSION     DASWBY010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167237"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /gene="amrB"
                     /locus_tag="VF870_07360"
     CDS             complement(<1..254)
                     /gene="amrB"
                     /locus_tag="VF870_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX9252043.1"
                     /translation="MKIRHQQVAGYFYPAEKEKLQNDISLLFKVTKPEKSFNKIFGIV
                     SPHAGYIYSGKTAAYAYNLLKDKEYKTVIIISPSHAEYFPG"
     gene            complement(251..814)
                     /gene="amrA"
                     /locus_tag="VF870_07365"
     CDS             complement(251..814)
                     /gene="amrA"
                     /locus_tag="VF870_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX9252044.1"
                     /translation="MKISTEEKDILLKAARQSIGSLFDGKEPEQPDYQKFPLLKEKLG
                     AFVTLTINHQLRGCIGYIIGHEPLFDTICSAAIHAAVNDPRFSVLKKSEYDKIHIEIS
                     ILSEFVPIKSYDEIIIGKHGLYLDEGGGGLLLPQVATEHKMNREEFLTAICHKSGLYG
                     EYWKERMLKIKVFTAEVFSEEETEKTK"
     gene            complement(909..1736)
                     /locus_tag="VF870_07370"
     CDS             complement(909..1736)
                     /locus_tag="VF870_07370"
                     /inference="COORDINATES: protein motif:HMM:NF027573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4905 domain-containing protein"
                     /protein_id="HEX9252045.1"
                     /translation="MKLKKLYKHDNGKQIWRILPTDNNKVILEEREINTKEVFFSCLE
                     VESGKKIFKNYQLDEKNWIGIESIYNDIIFFHSYGKPDMPAHKSIIAFDINSQSILWQ
                     NDNYVFSFVYADKVYCFQQRFESKVFFALDYLTGNVIEDFGNDVSVINQLKEQYDAKF
                     WEQNYLFPEYFNRSGQAEKDYQKYLQKVLTDKVIKGEVSYLLIDDLLMYNYHEVNKTN
                     TLNNEFAVIDLTKNKILLKETLDKNLVNLMPESFFVKDNFLFLIVDKTKLLVYSIME"
     gene            complement(1840..3219)
                     /locus_tag="VF870_07375"
     CDS             complement(1840..3219)
                     /locus_tag="VF870_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEX9252046.1"
                     /translation="MKPKTIIEPFKIKSVEPIRFTTREEREVLLKEAGYNPFLLPADD
                     VLIDLLTDSGTSAMSAKQWAGIMEGDESYAGSKSFYRFEAVIKSITNLKFIIPTHQGR
                     AAEKILFSVVGGPGKYFPNNTHFDTTRANVEFSGAEAVDLLNEVGKHPEIRADFKGNM
                     DVEKLEAFIKEKGVENIPLCMITITNNSGGGQPVSMQNIKDVKAVCTKYGIPLFLDAC
                     RFAENAYFIKMREKGYENKTPLEIAQEMFSYADGVTMSAKKDALVNIGGFLALNDDEL
                     AMKCRNLLIVTEGFPTYGGLAGRDLEAVAQGLLEIVDEHYLQYRIRSVEYLGERLVAA
                     GVPIIEPPGGHAIYIDAKRFLPNIPADQYPGQSIVAELYLEGGVRAVEIGSVMFGRYD
                     KNGKLIPSMMELVRMAIPRRVYTQSHIDYLIEVVIEVYKNREKLKGYKITYEAPMLRH
                     FTARFEPIN"
     gene            3376..4188
                     /locus_tag="VF870_07380"
     CDS             3376..4188
                     /locus_tag="VF870_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEX9252047.1"
                     /translation="MENQDTIQSEQPTNEMEELSHTDKIVGVISEPSTLFTKLVHLNS
                     KVTDWLLPLTALIVVAIAATFIYMSNPEIKLEMQQQQEKAMREQFDKMVESGQITREQ
                     ADEQMDKTMQMMGGSGMMQVFSSIGILVIMLLWFFVFTTIAFLIAKFVFKGDGSYSQA
                     MTAMGLPLYITVLQTVILIILGMLMGKMLSGLNPAALTGMDIKSFPGFLLSRLDVFSI
                     WFYVVVGIAFAKMFKSDNVKKYIFTSVGLWLVFMFIIFGLGKVSPIFANMIR"
     gene            complement(4225..>5582)
                     /gene="priA"
                     /locus_tag="VF870_07385"
     CDS             complement(4225..>5582)
                     /gene="priA"
                     /locus_tag="VF870_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX9252048.1"
                     /translation="RRVLKGKSKIVIGARSALFAPLSNIGLIVVDEEHDASYKSADMI
                     PKYNARDAAVVLGNIFSCPVLLGSATPSIESMYNAETGKYKLLSLLTRIDDAKLPKIT
                     FVNISHEKNKIKSETVFSKILIDKIDDRLKKNEGVIILQNRRGFSTQIFCNDCGEVEM
                     CENCSVPMTFHINQNKIQCHYCGLIKPVPGACTHCGSINIKYFGTGTERVEDELQFYF
                     PNARIERIDSDSITKKSYLSNLLLGFSKGDIDILVGTQMVSKGLDFPRVTLVGVISAE
                     TTLWLPDFRADERTFQLLTQVSGRAGRSKVEGEVIIQTYNEKHFVLRKVLENDYKGFY
                     AKEKADREKMGYPPFTRIALIETKDTSMDKAKGAAYDFYKEIIKYKNFLKISDPTTAQ
                     IFKLLNNYRYHILIKSYKEKDPGGAILRKAILDAWTEFNKKSRYRDVKLYYDVDPQSI
                     M"
BASE COUNT         1707 a         1058 c          880 g         1937 t
ORIGIN      
        1 cctggaaaat attctgcgtg actaggagaa attataatta cagttttata ctccttatct
       61 tttaaaagat tataggcgta tgctgcggtt tttcctgaat aaatatatcc agcatgcgga
      121 gagacaatcc caaatatttt attaaacgac ttttctggtt tagtgacttt aaaaagtaaa
      181 gagatatcat tttgtaattt ttctttttct gccggataaa aatatcctgc tacttgctga
      241 tgcctgattt tcattttgtt ttctccgttt cttcttcaga aaatacttca gcagtaaaga
      301 ctttaatttt taacattcgc tctttccagt attcaccata taatcctgat ttatgacaga
      361 ttgctgtaag aaattcctcg cggttcattt tgtgctcagt tgctacctga ggcagtagta
      421 atcctccgcc gccttcatcc agatataatc catgcttacc aattattatt tcatcataac
      481 ttttaatggg aacaaattct gaaagtattg aaatttcaat atgaatttta tcatattcag
      541 atttctttaa gacagaaaat cttggatcgt tgactgcggc atgaatagct gctgaacaga
      601 ttgtatcaaa caaaggctca tgaccaataa tatacccaat gcatccgcgt aactgatgat
      661 tgatagttaa agtaacaaac gcaccaagtt tttctttaag caatggaaat ttttgataat
      721 cgggttgttc gggttctttt ccgtcaaaca aagaaccgat tgactgacgt gcggctttaa
      781 gaaggatgtc cttttcttct gttgagattt tcatattatt tttttctgaa gtcatcacga
      841 gcgaggcgaa gtgatctgtt cataaatcag attgcttctc ccgacttcgt cggtatcgca
      901 atgactatct attccataat cgaataaacc aacaactttg ttttatcaac aatcaaaaag
      961 agaaaattat ctttaacaaa aaatgattcc ggcattaaat taactaaatt tttatccaaa
     1021 gtttctttta acagaatttt atttttagtt aaatcaataa cagcaaattc attatttaat
     1081 gtatttgttt tgttaacttc gtgatagtta tacattaaca aatcatcaat caataaataa
     1141 ctaacttcgc ctttaataac tttatctgtt aaaactttct gcaaatactt ttgataatct
     1201 ttttcggcct ggccgcttct gttaaaatat tccgggaaaa gataattttg ctcccagaat
     1261 tttgcatcat attgttcttt tagttgatta ataacagaaa catcattacc gaaatcttct
     1321 ataacattcc cagttaaata atctaaagca aaaaaaactt tcgattcaaa tctctgctga
     1381 aagcaataaa ctttatcagc ataaacgaaa gaaaatacat aattatcgtt ttgccataga
     1441 atagattgag aattaatatc aaatgcaata atgctcttat gtgccggcat atcaggcttg
     1501 ccgtaagaat gaaaaaatat gatatcatta taaattgatt ctattccaat ccagtttttc
     1561 tcatccagtt gataattttt aaatatcttt ttaccggatt ctacttccag acagctaaag
     1621 aaaacttctt tggtattaat ctctctttct tccaaaataa ctttattgtt atctgtcgga
     1681 agaattctcc agatctgttt tccgttatcg tgtttatata gtttttttag tttcatttta
     1741 tttcttagtc attgcgagcg agtttaggag cgaagcaatc ttaatttcta tcgtagattg
     1801 cttcgtcccg atcaaatcgg gactcgcaat gacaatctct taatttatag gttcaaacct
     1861 tgctgtaaag tggcgaagca tcggagcttc ataagttatc ttataacctt taagtttttc
     1921 gcgattctta taaacttcaa ttacaacttc aattaaataa tcaatatggc tttgtgtata
     1981 aactctgcgg ggaattgcca ttcgcacaag ttccatcatt gaaggaataa gtttcccatt
     2041 tttatcatac ctgccgaaca taacacttcc aatttcaaca gcgcgaactc cgccttcaag
     2101 ataaagttca gctacaatcg attgcccggg atattgatct gcaggaatat ttggtaaaaa
     2161 tctttttgca tcaatataaa ttgcatgtcc acctggcggt tcaataatcg gaactccggc
     2221 tgcaactaat ctttcaccaa gatattcaac actacgaatt ctatattgta aataatgttc
     2281 atcaacaatt tccagcaaac cttgtgcaac agcttcaaga tcgcgccctg caagtccgcc
     2341 ataagttgga aaaccttctg taactattaa aagatttcta cacttcatcg caagctcatc
     2401 atcattcaat gcaagaaatc cgccgatgtt tacaagtgca tctttctttg cgctcattgt
     2461 tactccatcc gcataagaaa acatttcctg tgcaatctct aacggagttt tattttcata
     2521 acctttttct ctcatcttta taaagtaagc attctctgca aacctgcacg catccagaaa
     2581 aagaggaatt ccatatttag tacaaactgc ttttacatct ttaatattct gcatagaaac
     2641 cggctgtccg ccgccggaat tattagtaat ggttatcata caaagcggaa tgttttcaac
     2701 tcctttttct tttatgaatg cttcaagttt ttcaacatcc atatttcctt taaagtcagc
     2761 acggatttcc ggatgcttcc caacttcatt tagaagatca acggcctctg ctccgctaaa
     2821 ctcaacatta gcacgcgttg tatcaaagtg tgtgttgttt ggaaaatatt ttccaggtcc
     2881 accaactact gaaaataaaa ttttctctgc tgctctaccc tgatgtgtag gaattatgaa
     2941 tttaagattt gtaatacttt taataacagc ctcaaacctg taaaaacttt tggagccggc
     3001 atatgactca tcgccttcca taattcctgc ccactgtttt gcgctcattg cagaagttcc
     3061 gctgtctgtt aataaatcaa tcagtacatc atctgccgga agaagaaatg gattataacc
     3121 tgcttctttt aacagtactt ctcgttcttc acgcgttgta aatcttattg gttcaactga
     3181 tttaattttg aaaggttcga tgatggtttt gggtttcata aattatccat aaaaagaatt
     3241 aattgaaatt ttcttgaaca aatttacaaa aagatgattc ttatttctca tcatatttga
     3301 aagattatta ttgccttttg attgattcct aaatagtttt ccatagtgtc atttcaaaaa
     3361 taatcaggag tagagatgga aaaccaagat actattcaat ccgaacagcc cacaaatgaa
     3421 atggaggaac tatcgcacac cgataaaatt gttggtgtca tttcagaacc atcaacattg
     3481 ttcactaaac tggtgcactt aaattcaaaa gtaactgact ggctgctccc tcttacggcc
     3541 ctgattgtcg ttgctatagc cgcgacattc atctatatgt caaacccgga aataaaattg
     3601 gaaatgcagc agcagcagga aaaagcaatg cgtgaacaat ttgataaaat ggttgaaagc
     3661 ggtcaaatta ctcgtgaaca agctgatgag cagatggata aaaccatgca aatgatgggt
     3721 ggttcaggta tgatgcaggt cttttcatca ataggaattt tggtgattat gctgctgtgg
     3781 ttttttgttt ttacaacaat agcattttta attgcaaaat ttgttttcaa aggagatggc
     3841 agttactccc aggcaatgac agcgatgggt ttgccacttt acattactgt tttgcagact
     3901 gtaattttaa ttattctcgg aatgcttatg gggaaaatgc tatcaggact taatcccgcc
     3961 gcattgaccg gaatggatat aaaatcattt cctggatttt tattaagcag acttgacgtt
     4021 ttttcaatct ggttttatgt agtagttgga attgcatttg caaaaatgtt taagtctgat
     4081 aatgtgaaga agtatatttt tacttctgtt ggactttggc ttgtatttat gtttataatt
     4141 tttggattag gaaaggtttc tccaattttt gcaaatatga ttagatagaa ttttctgcta
     4201 gaggaacggt cgggagaccg ttccctacat tatgctctgt ggatcaacat cgtagtaaag
     4261 tttaacatcc ctgtaacgcg attttttatt aaactcagtc caggcatcta aaatcgcctt
     4321 tcttaaaatt gccccacccg gatctttttc tttataactt tttatcaaga tgtggtagcg
     4381 atagttattt aataatttaa atatttgtgc ggtagttgga tcagaaattt ttaagaagtt
     4441 tttgtatttg ataatttctt tgtaaaaatc atatgcggcc ccttttgctt tatccatact
     4501 tgtgtctttt gtctcaatta aagcaatacg agtaaatggc ggataaccca ttttttcccg
     4561 atcggctttt tcttttgcat aaaatccttt gtaatcattt tccaaaactt ttcttagcac
     4621 aaaatgtttt tcattgtatg tttgaattat aacctctcct tcaaccttgc ttcttcccgc
     4681 tctaccggaa acctgagtaa gcagttggaa agttctttca tcagcccgaa aatccggtaa
     4741 ccacaatgtt gtttctgcag aaattactcc aactaaagta acccgcggaa aatccaatcc
     4801 tttagaaacc atttgagttc caacaagtat atcaatatcg cctttgctaa aacctaaaag
     4861 taaattactc aaataagatt tttttgtaat tgaatcagaa tcaattctct cgatgcgcgc
     4921 gttcggaaaa taaaattgta attcatcttc aacacgttct gttccagttc caaaatattt
     4981 tatgttgatc gaaccgcagt gcgtgcatgc accgggcaca ggttttatta atccgcaata
     5041 atggcactgg attttatttt gattaatgtg aaatgtcatc ggcacagaac aattttcaca
     5101 catttcaact tcaccgcaat cattgcaaaa tatttgagta gaaaatccgc gcctgttttg
     5161 aagaattatt acaccttcat ttttctttaa tcgatcatca attttatcta taagaatttt
     5221 tgaaaaaaca gtttcagatt taatcttatt tttttcgtgt gatatattca caaaagttat
     5281 cttgggaagt tttgcatcat caattctagt aagaagcgaa agtagtttat attttccggt
     5341 ttcagcattg tacatgcttt caatcgatgg agttgcagag ccaagcagaa caggacaaga
     5401 aaaaatattt ccaagcacaa ctgcggcatc cctagcattg tatttgggaa tcatatctgc
     5461 gctcttataa cttgcatcgt gttcttcatc aacaactatc aacccaatat tacttagcgg
     5521 cgcaaataat gctgagcgtg caccaattac aattttagat ttccctttta aaacccgcct
     5581 cc
//
LOCUS       DASWBY010000411         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_242990, whole genome shotgun sequence.
ACCESSION   DASWBY010000411 DASWBY010000000
VERSION     DASWBY010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242990"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF870_07390"
     CDS             <1..297
                     /locus_tag="VF870_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX9252049.1"
                     /translation="SVDAILNIHNLAIGKTIPDITFFIDIPNNIALARKKNIAHSDLD
                     RIEVSDDTFYERVREGYLYLAKKEKRFRVIDGTRNIKQIHEQIINEIKSIEGVK"
     gene            298..>1545
                     /locus_tag="VF870_07395"
     CDS             298..>1545
                     /locus_tag="VF870_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9252050.1"
                     /translation="MKSRRIILISSLTIIVFFTFGFVVSKSDIYFEISKNLELFGKVY
                     KEISFNYVDDIDPQEFMRAGIKGMLSKLDPYTVFIDEKNQDNIDLLTNGKYGGVGISI
                     GIRNDKVTILEVLDGYSAQKQGIRIGDVISKIGDTKVTPEIIDDISSLVKGEPGTPVQ
                     MKILRPSVTDTLEFNLIREEVIVKNLVFADFYPENSNNVYLKLSNFSRSAGDEIRSAL
                     KRLKDKKEIKSIVFDLRGNPGGLLDVAVDICDKFLKKDLLIVSTRGKELSSEKKYYAL
                     EEPLVGKEKLIVLVNGNSASASEIVAGAIQDHDRGVILGTQSFGKGLVQTITPLNFNT
                     SLKITTAKYYTPSGRCIQRIDYSKDNQVIPDPDSLKQGEFSTDNKRVVYSAGGITPDT
                     TVKNGIESDLLKDLLAKGIIFDFA"
BASE COUNT          562 a          203 c          295 g          485 t
ORIGIN      
        1 tctgttgacg ctattctaaa tattcataat cttgctatag gtaaaactat tccggatata
       61 acttttttta ttgatatccc caacaatatc gcgcttgcac ggaagaaaaa tattgctcat
      121 tccgatttag atagaattga ggtttcggat gatacttttt atgagcgggt tagggaagga
      181 tatctttact tagcaaaaaa agaaaaacgt tttagagtta ttgacggcac acgaaacatt
      241 aaacaaatac acgaacagat tattaatgaa attaaatcca tagaaggtgt taaataaatg
      301 aagtcacgaa gaataatatt aatctcatcg ctaacaatta tagtcttttt tacttttgga
      361 ttcgttgtaa gtaaaagtga tatatacttt gaaataagta agaatcttga actatttggt
      421 aaagtctata aagaaatttc attcaactat gttgatgata ttgatcccca agagtttatg
      481 cgtgcaggta taaaaggtat gctaagcaaa ctcgatcctt acacagtttt tattgatgaa
      541 aagaatcagg ataatattga tcttttaact aatggtaaat atggtggtgt tggaatctca
      601 attggtatta gaaatgacaa agttacaata ctggaagtgc ttgatggtta ttcagcccaa
      661 aagcagggaa tcagaatcgg tgatgtaatt tcaaaaattg gagatacaaa agttactccg
      721 gaaattatag atgacatttc atcacttgtt aaaggtgaac cgggtacacc tgtacaaatg
      781 aagattttaa gaccgagtgt aacagatact ttggaattta atcttataag agaagaagta
      841 attgtaaaaa atcttgtctt tgcagatttc tatcctgaaa attcaaataa tgtatattta
      901 aagttatcga attttagccg ctctgcgggc gatgaaatta gaagtgctct aaagagactt
      961 aaggataaaa aagagattaa atcaatagtt tttgatttga gagggaatcc aggcggattg
     1021 ttggatgttg ctgtagatat ttgtgataaa tttttgaaga aggatttatt aattgtttca
     1081 accaggggta aagaactaag cagtgaaaaa aaatattatg cgttagaaga gcctctggtt
     1141 ggaaaagaaa aactaattgt tttggttaat ggaaacagtg cttctgcttc tgaaattgta
     1201 gcaggtgcta tacaggacca tgatagaggt gtaatacttg gaacccaatc atttggtaaa
     1261 ggattagttc aaaccataac tccattaaat tttaatactt cgctaaaaat aactactgca
     1321 aaatattata cccctagcgg aagatgtata cagagaatag attactcaaa agataatcag
     1381 gtaattcctg atccggattc tttaaagcaa ggtgaatttt ccactgataa caaaagagta
     1441 gtttatagcg ctggtggaat aacaccagat acaaccgtaa aaaatggaat cgaaagtgat
     1501 ttattgaaag accttttagc aaaaggcatt atctttgatt ttgct
//
LOCUS       DASWBY010000412         8019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_167272, whole genome shotgun sequence.
ACCESSION   DASWBY010000412 DASWBY010000000
VERSION     DASWBY010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167272"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VF870_07400"
     CDS             <1..395
                     /locus_tag="VF870_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9252051.1"
                     /translation="GITFVGKTDSNHWITMDGPESFGGSDAGIRPKELLMLSLGGCTG
                     SDVTSILGKKKIKLDGFEINITADSADEHPQVFTRMHLEYVFYGKNLPIKDIERAIEL
                     SLTKYCSVTAMLQKAMPIEHSYKIVEVE"
     gene            complement(458..808)
                     /locus_tag="VF870_07405"
     CDS             complement(458..808)
                     /locus_tag="VF870_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9252052.1"
                     /translation="MAEHLIAHCEIPSTDLDKSKDFYHKVLGWEFKPFGNGYLLFNNH
                     KGTMVGLRKVDEVAKGEATVFHVNMPDIDSILKKVKENGGAVKRTKTVIPAMGWYALF
                     NDPDGNTLGLYQKS"
     gene            954..1676
                     /locus_tag="VF870_07410"
     CDS             954..1676
                     /locus_tag="VF870_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252053.1"
                     /translation="MKSIILFLLVFILFSFNNSFSQQEDHASEINSSVPELFDFHEVV
                     YPLWHTAYPNKDYAMFKKLLPDVNAGVEKIYAAKLPGILRDKENEWNEGLNKLRTSVK
                     DYNKSCEENNEAAMLTSAEELHSNFEMLVRIVKPVTEEVDEFHKVLYMIYHHYGPNKN
                     TEELNKAIDDLMMRADELKNCVLPKWATDKKNDFAEAADKLYNSTKELQTLKNTKADY
                     KQINEGIEKVHTDYQKLEALFD"
     gene            complement(1764..2321)
                     /locus_tag="VF870_07415"
     CDS             complement(1764..2321)
                     /locus_tag="VF870_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX9252054.1"
                     /translation="MKRNPIILKKETTALLIIDLQERILPVIRNYENVLENTVKLIKG
                     FKAMQLPIYFTEQYPKGLGTTSSKILEELSGYTAYQKMSFSCSGAENLFEEFHKKKLS
                     QIVVCGVESHVCVQQTVLDLISNDHQVNLAADAVSSRKEIDYNISLERMRTLGAEITT
                     TESILFELLEVCGTPEFKEVSKIVK"
     gene            complement(2470..3306)
                     /locus_tag="VF870_07420"
     CDS             complement(2470..3306)
                     /locus_tag="VF870_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252055.1"
                     /translation="MSFYPQPYKYQCGPFALKYALVMLGRFESENQIGIKAGSTWWYG
                     TDEIGLAKAAKFYNCKMKYFRREKPEDAIKILIEQIKKGYPCILSVDNWEHWLTVVNF
                     QQRKFIVTDSSLDKVVTIYTPNQLLKRWKYYDEDDDDISYDGYAIIPQYKVTTKAKFT
                     LEQARFVMDTRNQKLAKKWDTYFNDMIAICHPRHANATHLISFRDFLRRFEKILVTQV
                     ANWHGSPNHRELKKILSNFQFMADVYDLVIHADEQKKALIDLSSILMMYACGKYGMDE
                     IY"
     gene            3389..4447
                     /locus_tag="VF870_07425"
     CDS             3389..4447
                     /locus_tag="VF870_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9252056.1"
                     /translation="MKAKKIKVLVLYNEAHPEFYHKPVEPAPEPDFKPYFEVDNLTPM
                     EEYGIIVKKLKRVGFDAYELNLKDNIYSLLDLIKQNPPDVIFNFAEIFKEEPRLEMNL
                     VGLYELLGIPYTGAPAITLANCQSKFLTKKLLNYHGIKTAKFQLFDKIPNEFKLETKF
                     PVIVKPAYEDASVGIENESIVYNLKGLEKRLDYVFHHFSQPALIEEFIEGREFNVSVT
                     GDLKPKVLPISEIDFSRMPDHLHNIVSYQAKWDPQHESYHKTIPICPAKISKSLEAKL
                     KYTAISAFKIMGTRDYARVDMRVTSDEEVYVLEVNPNPDLTEGAGFMRSAHAAGLSYA
                     QALKKIVMLAYERGKRTM"
     gene            4568..5218
                     /locus_tag="VF870_07430"
     CDS             4568..5218
                     /locus_tag="VF870_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252057.1"
                     /translation="MYSIKYLVIVFVFSAALVYPQSKYKVEGSLGIISPFNSSSGLTG
                     SLQINHSLNENLQLFLNGMYGQWDKYNIYYIPEFSSSEYQYNGPYKTYSADNHSLFSV
                     NLGARFFLHENKIINLLFDAQIGLSMLSYNEYDLNEFEYPTGQIVLVPDLNSAVEVNE
                     TLFALGFGPVFERKINPSLSLYLSAKINTMLNAGDTELLSKRGTYFLVSAGFTHAI"
     gene            complement(5285..5809)
                     /locus_tag="VF870_07435"
     CDS             complement(5285..5809)
                     /locus_tag="VF870_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HEX9252058.1"
                     /translation="MKKITPSFWVVTLMVFVAAFVRLLPHPPNFAPIAAMALFGGAYF
                     NKKSFAFAIPLVALFLTDLIIGIYSYAWIVYLSFALIVVLGIIMLKKVSVKNVVIASL
                     TASVSFFAITNFGVWALGTLYPKTPAGLMESYIAAIPFFQYSLIGDLFFVVVMFGVYE
                     LVKHKVPALAKVKA"
     gene            complement(5945..7930)
                     /locus_tag="VF870_07440"
     CDS             complement(5945..7930)
                     /locus_tag="VF870_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9252059.1"
                     /translation="MHKTKFLFSLFAIFLCSYFSIAQTSVIQKTDSAGYYKLNDVVIT
                     ATKTPANTLELANSISVIDSNQISNSNSNNVFDVLKNETGISFTRQGGNGTLSNLYIR
                     GANSSHTLVLIDGVEVNLTNDPSGVYDFSALTTDNIERIEVLRGPQSTLYGSDALAGV
                     INIISKKGNGSPKFSLLTEGGTYNSYKALLGLNGSLKKLNYSISLSRTGSDGFSVASE
                     KYGNTEKDSYNFNNASALIGYSFTENAEINLYTRFLKSQSDNDQFGGIYGDDPTYLTN
                     QEEFSIRGEGKFKLFDGLWNQKLGLTFFRNVRNNSYDTSSASINYPFYINDYSWAHYD
                     GRKYKIDWQNDFQVNNLNLITTGLEFEIEESASEYYALNYVPDPYSPDIESVIPMQNA
                     STFGIFIQDQFKFDESFFVTLGTRYDDHSKFGSQFTYRIAPAFMLWEINTKFKATIGT
                     GFKAPSLYYLYDPAYGNDNLNPEESFGWDFGIEQYLFAQNLSFGATYFYNKFTDMFGF
                     DYVTFKTINIKQAVTKGFEVYIEAKPINELTFKANYTFTDARDTSPDSPEFEKKLLRR
                     PESKLGFYTSYSFIPKANINAELIWVGVREDIDFSTYQRTELKGYVLINLAAHYDVFD
                     FLRLNVRAENLLDTDYEEVYGYGTAGLSFYGGIKLSL"
BASE COUNT         2695 a         1319 c         1339 g         2666 t
ORIGIN      
        1 aaggaataac ttttgtaggt aaaactgatt caaatcactg gattacaatg gatggtcccg
       61 aaagttttgg tggaagtgat gccggaattc gtccaaaaga attattaatg cttagtcttg
      121 gcggatgcac tggaagtgat gttacaagta ttcttggaaa aaagaagatt aaactcgatg
      181 gctttgaaat taatattacg gctgattctg cagatgagca tccacaggtt tttaccagga
      241 tgcatcttga atatgttttt tatggaaaga atctgccaat taaagatatt gaaagagcaa
      301 ttgaattatc tttaacaaaa tattgcagtg ttacagctat gcttcaaaag gccatgccaa
      361 ttgaacatag ttacaaaata gttgaagttg aataaataat atttgaggaa ttggagtaat
      421 gtaaagttaa attacattac tccttaaatt cttaggatta acttttctga taaagaccta
      481 aagtatttcc atcaggatca ttgaaaagcg cgtaccaacc catggcagga attacggttt
      541 ttgttctttt aacagctccg ccattttctt ttaccttctt taatatcgaa tcgatatccg
      601 gcatattaac atgaaaaaca gttgcctcac ctttggcaac ttcgtcaact tttcttaatc
      661 cgaccattgt gcctttatga ttattaaaaa gtaagtatcc gtttccaaat ggtttaaatt
      721 cccagccaag aaccttatga taaaaatcct ttgatttatc caaatctgtt gatggaattt
      781 cgcaatgtgc aattaaatgt tcagccataa ataatccttt ttaaaatttg agtttttgca
      841 taggtgaaat tacaagttag tgatttgtaa ttcaagaaaa atttgtatgc ggagaaatga
      901 aattcattca gtaattttgt atgtatttta tcacatattc tgaaggggct tttatgaaaa
      961 gcataatatt atttttactg gtatttattc ttttttcgtt caataactca ttttcacaac
     1021 aagaagatca tgcatccgaa attaattcat ctgtgccgga actgtttgat tttcacgaag
     1081 tagtttatcc actttggcat acagcatatc caaacaaaga ttatgcaatg tttaaaaaac
     1141 ttttaccgga tgtgaatgct ggagttgaaa aaatttatgc tgccaaactt ccaggaattt
     1201 tacgcgataa ggaaaatgaa tggaatgaag gtctgaataa acttcgaaca tctgtaaagg
     1261 attacaataa atcctgtgag gaaaacaacg aagcggctat gttaacatct gcagaagagc
     1321 tgcattcaaa ttttgaaatg cttgttagaa ttgtaaaacc tgttacagaa gaagttgatg
     1381 aatttcataa agtgctttat atgatttatc atcattacgg gccaaataaa aacacagaag
     1441 aactaaataa agcaatcgat gatttaatga tgcgagcaga tgagttaaaa aattgtgtcc
     1501 ttcctaaatg ggcaacagac aaaaaaaatg attttgcaga agctgccgat aagctataca
     1561 attcaacaaa agaattacaa acattaaaaa atactaaagc cgattataaa cagataaatg
     1621 agggaattga aaaagttcac acagattatc aaaaattaga agcactcttt gattaggatt
     1681 taagggttat tcgcgcgaaa gaaggaatct gagactttaa attaaaatgg atccccgcct
     1741 acgcggggat gactttttta aacttactta acaattttag aaacttcttt aaactctggc
     1801 gttccacaaa cttctaataa ttcaaaaagt attgattctg tagtagtaat ttctgcacca
     1861 agtgttctca tgcgttccaa agaaatattg taatcaattt cttttcttga tgaaactgca
     1921 tctgcagcta aattaacctg gtgatcattt gaaattaaat ccaatactgt ttgctgcaca
     1981 caaacgtgag attcaactcc gcatacaaca atctgagata atttcttttt gtggaattct
     2041 tcaaaaaggt tttctgcacc ggaacaacta aaactcattt tttgataggc agtataacct
     2101 gaaagttctt ccagaatttt ggaagaagtc gttcccaatc cttttggata ttgttcagtg
     2161 aaataaattg gcagctgcat tgctttgaaa cctttaatca gtttaacagt attctctaaa
     2221 acattttcat aatttctgat taccggaagg atgcgttcct gcaaatcaat aattaaaaga
     2281 gcagttgttt cttttttaag aatgatagga tttcgtttca tagtattttg tagttttgat
     2341 cttaaactta gactgtcagt gcgaatcccg actcgtcggg atgaagcaat ctttgttatt
     2401 tattcgattg cttcgctcga agactcactc gcaatgacca ttttttgtct taaacttgaa
     2461 cctgaacttt tagtatatct catccattcc atacttaccg caggcgtaca tcattaaaat
     2521 tgatgaaaga tctatcaatg cttttttctg ttcatcagcg tggattacaa gatcataaac
     2581 atccgccata aactgaaagt tgcttagtat cttttttaat tctctatgat ttggtgaacc
     2641 atgccagtta gcaacctggg tcaccagtat cttttcaaat cttcttaaaa aatctctgaa
     2701 ggaaattaag tgagttgcat tagcatgtct tggatggcat attgcgatca tatcattaaa
     2761 atacgtatcc cattttttag caagcttttg attgcgtgta tccattacaa atcttgcctg
     2821 ctctaaagta aactttgctt tagttgtaac cttgtactgc ggaattattg catagccatc
     2881 ataacttata tcatcatcat cttcatcgta gtacttccat cgtttcagta attggttggg
     2941 agtataaatt gtaacaactt tatcaagtga gctatcagtt acaataaatt ttctttgctg
     3001 aaagtttaca actgttaacc agtgttccca gttatcaaca ctaagtatgc agggataacc
     3061 ttttttaatt tgttctatta aaatttttat tgcgtcttcc ggtttttctc ttcgaaaata
     3121 tttcatcttg cagttataaa actttgcagc tttagctaaa ccgatttcat cggtgccata
     3181 ccaccacgta ctgccggctt ttattccgat ttgattttcg ctttcaaatc tgccaagcat
     3241 tacaagagcg tatttaagtg cgaagggtcc gcattgatat ttatagggtt gtgggtaaaa
     3301 actcatttta aattttattg ataatgaaac tatgtaaaca ttatcaaatg aagttttttt
     3361 tacaagtgat tttgggtatt ttttaggtat gaaagcaaaa aaaattaaag ttctcgttct
     3421 ttacaacgaa gctcaccccg agttttacca caaacctgtt gaaccagctc cggaacctga
     3481 ttttaagcca tattttgagg tagataatct tactccaatg gaagaatatg gaattattgt
     3541 aaaaaagcta aaaagagtgg gttttgatgc atatgagttg aaccttaaag ataacattta
     3601 cagccttctt gatttgataa agcaaaatcc gccggatgta atctttaatt ttgcagagat
     3661 ttttaaggaa gagcccagac ttgagatgaa tttggtggga ttatatgaac ttcttggaat
     3721 tccatataca ggtgctccag cgataacact tgcaaactgc cagagtaaat ttcttacaaa
     3781 aaaactgctc aattatcacg gaattaaaac agcaaaattc cagttgtttg ataaaattcc
     3841 aaatgagttt aaacttgaaa ctaaatttcc ggtaattgtt aaacccgctt atgaagatgc
     3901 aagtgtcggg attgaaaatg aatctattgt atataacctt aaaggcttag aaaaaagact
     3961 cgactatgtc tttcatcatt ttagtcagcc ggccttgatt gaagagttta ttgaaggacg
     4021 cgagtttaat gtttctgtaa caggagattt gaaaccaaaa gttttaccga ttagtgaaat
     4081 tgattttagc agaatgccgg atcacttaca taacattgta agctaccagg caaaatggga
     4141 tccgcagcac gaatcatatc ataaaacaat accaatctgc cctgcaaaaa tttctaaaag
     4201 tttggaagca aaacttaagt acacagcgat ctcagcattt aaaattatgg ggactagaga
     4261 ttacgcacgt gttgatatgc gtgtaacaag tgatgaagaa gtttatgtgc tggaagtaaa
     4321 tccaaatccg gatttaacag aaggtgccgg atttatgcgc tctgctcacg cggcaggact
     4381 gagttatgca caagctctga aaaaaattgt tatgctggct tatgaaagag ggaagagaac
     4441 gatgtaaatt gtgtgttttt tgttgacctc aaaaactttt gcacttttat ttcccttcaa
     4501 atatataact tttagttagc taaaattttt cttttgaaaa gtttttagta agctgaaagg
     4561 gacaaaaatg tatagtatta aatatttagt gattgtattt gttttttctg ctgcactagt
     4621 ctatccgcaa agcaaataca aagttgaagg tagtcttgga attatatcac catttaactc
     4681 ttcatcggga ttgacgggat ctctacagat aaaccactct ttaaatgaaa atctgcaatt
     4741 atttttgaat ggtatgtacg ggcaatggga taaatacaat atttattata tccccgaatt
     4801 ttctagctct gagtatcagt ataatggacc atataaaaca tattctgccg ataaccattc
     4861 tttattttca gtaaatcttg gagcaaggtt ttttcttcac gaaaataaaa taataaatct
     4921 attatttgat gctcaaattg gtttatcgat gttatcatat aatgagtatg atcttaatga
     4981 gtttgaatac cctacaggac aaattgtttt agttcctgat ttgaattcag ccgttgaagt
     5041 aaacgaaact ttgtttgctc ttggatttgg accagtgttt gaacgaaaaa taaatccctc
     5101 tttaagtttg tatttatcag ctaagattaa tacaatgctt aatgcaggtg atacggaatt
     5161 attaagtaag agaggcacat attttttagt ttccgctggg tttacacatg ctatctaagg
     5221 cagggatata aaacaaaaaa cccgatccat catcgaatcg ggttttctac tgtaaactat
     5281 ttctttatgc ttttactttt gcaagcgccg gtactttgtg tttaactaat tcataaaccc
     5341 caaacattac aacaacaaag aataaatctc caattaaaga atattggaaa aatggaattg
     5401 cagcaatgta gctttccatt aagcctgctg gagtttttgg atataatgtt ccaagtgccc
     5461 aaacgccaaa gtttgtaatt gcaaagaagc ttactgaagc cgttagtgat gcaatcacaa
     5521 cattctttac acttactttt ttaagcatta taattccaag cacaacaatc aaggcaaagc
     5581 ttaaataaac aatccaggca taagaatata ttccaattat caaatctgtt aaaaataatg
     5641 caacaagcgg aattgcaaat gcaaaagatt tcttattaaa gtatgcacca ccgaataaag
     5701 ccattgctgc tataggtgca aagtttggag gatgaggaag taatcgcaca aaagccgcaa
     5761 cgaaaaccat cagtgtaact acccaaaagc ttggagtaat ttttttcatt tgatcaacca
     5821 tcgttttttc ctttcaataa aaatctggta aaagatattt cttgtaataa ttagttcaaa
     5881 gataaagttt attaacgtca aatgtcaatc cgcactcggc gaatcaaatg tcaaagtttt
     5941 gtcattaaag cgaaagctta attcccccgt aaaatgataa acctgcagtt ccgtaaccgt
     6001 aaacctcttc ataatcggta tctaaaaggt tttctgctct tacattcaat cttaagaaat
     6061 caaaaacatc ataatgtgcg gcaagattta taagcacata tcctttaagt tcggttcttt
     6121 gataagtgga aaagtcaata tcttcgcgaa caccaaccca aataagttca gcatttatgt
     6181 ttgcttttgg tataaaagaa tagctggtgt aaaatccaag tttactttca ggtcggcgta
     6241 ataatttctt ttcaaattca ggtgaatcag gacttgtatc tcttgcatca gtaaaagtat
     6301 aattcgcttt aaaagttaat tcatttattg gtttggcttc tatgtaaact tcaaatccct
     6361 ttgttacggc ctgtttaata ttaatagttt tgaatgttac ataatcaaac ccgaacatat
     6421 cagtaaactt gttgtagaaa taagttgcac caaaagataa attttgagca aagagatatt
     6481 gttctatgcc aaaatcccag ccgaaacttt cttcagggtt taagttatca tttccatacg
     6541 cgggatcgta taaataatat aaagatggag ctttaaatcc ggttccaatt gtggctttaa
     6601 actttgtatt tatttcccat aacataaaag cgggagcaat tctatatgta aactgtgaac
     6661 cgaatttact atgatcatca tatctggttc caagtgttac aaaaaaactt tcatcaaatt
     6721 taaattgatc ctgaataaat attccaaacg tgcttgcatt ctgcatcggg ataacacttt
     6781 caatatccgg agaataagga tcaggcacat agtttaaagc ataatattct gatgcggatt
     6841 cttcaatctc gaattctaac ccagttgtaa taagatttaa attattaact tgaaaatcat
     6901 tctgccaatc aattttatat tttcttccat cgtaatgtgc ccaggaataa tcattaatat
     6961 aaaacggata gttaatgctt gcagatgaag tgtcataaga attattgcga acatttctaa
     7021 aaaaagttaa acccagtttt tgattccaaa gaccatcaaa taatttgaat ttaccttctc
     7081 cacgaattga aaattcttcc tgatttgtta aataagttgg atcatcacca taaattccgc
     7141 caaactgatc attatcagat tgtgatttta gaaatcgtgt ataaagattt atctctgcat
     7201 tttctgtaaa gctataccca attaaagcag aagcgttgtt gaaattataa ctatcttttt
     7261 ctgtattccc atacttttct gaggcaactg aaaatccatc acttcctgtt ctgcttaatg
     7321 aaatagagta atttaatttc tttaaagatc cattaagtcc taatagtgct ttataggaat
     7381 tgtaagtacc gccttcagtc aataaagaaa attttggtga accatttcct ttttttgata
     7441 taatatttat tactcctgca agtgcatctg agccatatag agttgattgc ggaccccgta
     7501 aaacttctat tctttcaatg ttatctgtgg tgagggcaga aaaatcataa actccacttg
     7561 gatcatttgt aagattaact tcaactccat caattaatac taatgtatgt gatgaatttg
     7621 caccgcgaat gtaaagatta gacaaagttc cattgccgcc ttgtcttgta aaactaattc
     7681 cggtttcatt tttcaggaca tcaaaaacat tatttgaatt gctgttagaa atttgatttg
     7741 aatcaattac agagatagaa tttgctaatt caagtgtgtt agctggagtt tttgttgctg
     7801 taattacaac atcattaagt ttataataac cagctgaatc agttttttgg ataacggagg
     7861 tttgtgcaat agaaaaatat gagcaaagaa aaattgcaaa caaagaaaaa agaaattttg
     7921 ttttgtgcat tgaagttact ccttaaaaaa ggttaaaagt aacttcgcgt attaaaagtt
     7981 tagataaaac gaatggagat tataaaagac catcgttaa
//
LOCUS       DASWBY010000413         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_203160, whole genome shotgun sequence.
ACCESSION   DASWBY010000413 DASWBY010000000
VERSION     DASWBY010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203160"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VF870_07445"
     CDS             <1..1327
                     /locus_tag="VF870_07445"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9252060.1"
                     /translation="FPGIDLAGIFSVRTVPDARHIREWLDRDLLNTGMNEYSGVNTIG
                     KPKNAVVVGGGFIGLEMVENLIHRGLNVTLIEKLNQVMPPLDIEMARIVKRYMEKHGV
                     KVELNDGVAGFRKAKDGNLEVLTESGKVYSAEIIILAIGVKPETALAKMAGIEIGKRG
                     GIRVNDQMRTSNPDIFAVGDAVEVKDFVTGEWTLIPLAGPANRQGRIVADVLCGRNSH
                     YRGTQGTSICKIFDAAIAQTGVSEKTLKSLGDTDYEKVYTYPNSHAGYYPGAKMLIIK
                     TLFRKSDGKILGAQVLGEDGVPKRIDSFAMAIQMACTIYDLEESELCYAPSYSSAKDP
                     VNFAGMVAADVLRGDMPISHWDSIGNSFLLDVRNPPELEVESVKGAVNIPLPMLRSRL
                     NELPKDREILIICRSAQRAYYATRILLQNGFKARNISGGMTARSHCLVD"
     gene            1380..2579
                     /locus_tag="VF870_07450"
     CDS             1380..2579
                     /locus_tag="VF870_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252061.1"
                     /translation="MEEAPKSKINISDYFVEGTISKVLRKLKLEGEDDPSIIRKIIFF
                     VSLSWLPLLILTLIDSSALNTSIKIPLLLDFVIYVRVFIVLPLLFIAERMISAIIYKS
                     LIHFIQSGIVADNNIEDYKLNLKFFGKLKDSVIVDIIIIAGSYAIVILGWGNVWKYYN
                     LVAGYTTWQFSQTNEGSLSLSGYWYAFITIPVYLYFFIKLIWKFFLWAIVLFKLSRMK
                     LNLYPTDPDGSGGLGFLGQNQIFFGLIGFIQSSIFSAEIAHKILYTNAAITEFKFTIP
                     GFVVLAAFVLMSPMFFFIRKLSTTKLSGILDYGVIAHKYVSEFDDKWVKGNNPEGEKL
                     LGSGDIQSLMDLASSYEIIKTMFPLPIDIRRILTLLLIIIIPFSPLILFVIPLNELLK
                     ALANFIF"
     gene            2617..>2858
                     /locus_tag="VF870_07455"
     CDS             2617..>2858
                     /locus_tag="VF870_07455"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEX9252062.1"
                     /translation="MEIIESLLKQSVSIVTIILEFAAVLCVATGLITAIILLIKSKQI
                     QFSPLHNRLRLKFGGWLALALEFQLASDILKTAIAPT"
BASE COUNT          894 a          454 c          571 g          939 t
ORIGIN      
        1 gtttccggga attgatttag ctggaatatt ttctgtgaga acagttcccg atgcaagaca
       61 tattcgtgaa tggcttgacc gtgatttatt gaatactggt atgaatgaat acagcggcgt
      121 aaacacaatc ggaaaaccta aaaatgcagt tgttgtcggc ggtggattta taggtctcga
      181 gatggttgaa aatttaattc atcgcggact taatgtaacg cttattgaaa aactaaatca
      241 ggttatgccg ccgcttgata tcgaaatggc gcgaatagta aaacggtata tggaaaaaca
      301 cggcgttaaa gttgaactga atgacggtgt cgcaggattc cgtaaggcaa aagacggaaa
      361 tcttgaagta cttacagaat caggtaaggt ttattctgca gaaataatca tccttgcaat
      421 aggtgttaaa ccggaaactg cattagcaaa gatggccgga atcgaaattg gtaagcgcgg
      481 aggaatcaga gtaaatgatc aaatgcgtac ttctaatcct gatatctttg ctgttggtga
      541 tgcagttgaa gttaaagact ttgtcacagg tgagtggact ttaattccac ttgcaggtcc
      601 tgcgaacagg cagggaagaa tagttgcgga tgttctttgt ggtcgaaatt ctcattaccg
      661 cggtacacaa ggtacgtcaa tctgtaaaat ctttgatgca gcaattgcac agaccggtgt
      721 gagtgaaaag acactcaaaa gtcttggaga tacagattat gaaaaggtct atacttaccc
      781 gaattcgcac gcaggatatt atcctggtgc aaagatgctt atcataaaaa ctttattcag
      841 aaaatctgat ggcaagatac tcggcgcaca agtgctcggt gaagatggtg ttcctaaacg
      901 aattgattca tttgcaatgg caatacagat ggcctgcaca atttatgatc tggaagaatc
      961 agaactttgt tatgcgcctt cctatagcag tgctaaagat cctgtaaact ttgcaggaat
     1021 ggttgctgct gatgtacttc gtggagatat gccaatttcg cattgggatt caattggcaa
     1081 tagttttctt ttagatgttc gtaatcctcc agaacttgaa gttgaatcag ttaagggtgc
     1141 agttaatatt ccattaccaa tgcttcgttc tcgcttaaat gaattaccca aagatcggga
     1201 aatattaatt atctgtcgct ctgcacaaag agcatattat gcaacacgaa ttcttttaca
     1261 aaacggattc aaggctagaa atatatccgg tggaatgacg gcacgctcac attgtcttgt
     1321 ggattaaact taaaaaggat atttatcact taacagggat aacaaaggaa aataaataaa
     1381 tggaagaggc accgaaaagt aaaatcaata tttccgatta ttttgttgaa ggaaccataa
     1441 gtaaggtttt acgaaaactt aagcttgaag gcgaagatga tccgtcaatt ataagaaaaa
     1501 taattttctt cgtctcttta tcctggctgc cgctgctgat tctcacttta atcgattcat
     1561 cggctctaaa tacttcaata aaaattcctc tgctccttga ttttgttata tatgtaagag
     1621 tgtttattgt attgccctta ctatttattg cagaaagaat gattagtgct ataatctata
     1681 aatctttaat tcattttatt cagtcaggga ttgtagcgga taataatatc gaagattata
     1741 aattaaatct aaaatttttt ggtaagctta aagattctgt aattgtcgat attattatta
     1801 tcgcagggtc ctacgctata gtgattttag gatggggtaa tgtatggaag tattataatc
     1861 tggtggctgg ttatacgaca tggcaattct ctcaaacaaa tgaaggaagc ctttctttaa
     1921 gcggatattg gtatgcattc atcactattc ctgtttacct ttactttttc attaaactaa
     1981 tttggaagtt tttcttgtgg gcaattgttc tctttaagtt atcgagaatg aaactaaacc
     2041 tgtatcctac agatccggat ggctcaggcg gattaggttt ccttggtcag aatcagattt
     2101 tctttggact aattggtttt atacaaagct caatattttc tgctgaaatt gctcataaaa
     2161 ttctctacac aaatgctgct attacagaat ttaaatttac tatccctggt tttgtagtgc
     2221 ttgcagcttt tgtattaatg tctccaatgt tttttttcat aagaaagctt tctaccacta
     2281 aattatctgg tatacttgat tatggtgtaa ttgctcataa gtacgtgagt gaattcgacg
     2341 ataagtgggt taaaggtaat aatcccgaag gtgaaaaatt attaggttct ggagatatac
     2401 agtcgctaat ggatttggcc agcagttatg aaataataaa aacaatgttt cctttaccga
     2461 tcgatatcag aagaatttta actttacttt taatcattat aataccgttt agtccattaa
     2521 tattatttgt tattccttta aatgaactac ttaaagcttt agcaaatttt attttttaac
     2581 tatttgattc tggataaata tttgcaggag aaatttatgg agataattga atcattatta
     2641 aagcagtctg ttagtattgt gactattatt ctggaatttg cagcagtttt atgcgtggca
     2701 actggtttaa taactgcgat catcctatta attaaatcaa agcaaataca attttctcca
     2761 ttgcataaca gattaaggtt aaagtttggt ggatggctgg ctctggcact agaatttcaa
     2821 ttggcatctg atatactaaa aacagctatt gcgccaac
//
LOCUS       DASWBY010000414         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_730770, whole genome shotgun sequence.
ACCESSION   DASWBY010000414 DASWBY010000000
VERSION     DASWBY010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730770"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VF870_07460"
     CDS             complement(<1..405)
                     /locus_tag="VF870_07460"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEX9252063.1"
                     /translation="MFKVGKIEIDNAILLAPMEDVTDISFRLVCRELGADVVYTEFVN
                     SEGLVRSNAKTHNKLRIIEEERPVGIQIYGSSIESMVGAARIAEAENPDMIDINAGCW
                     VKNVVGCGAGSALLKDIPYLVELVKSVVDSVSL"
     gene            complement(420..1616)
                     /locus_tag="VF870_07465"
     CDS             complement(420..1616)
                     /locus_tag="VF870_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9252064.1"
                     /translation="MKNRSALVLIFLTVFIDLLGFGILIPILPSFSVKELHIDEASIG
                     IAIAIYSFVQFLFNPVLGKISDKYGRKPVIVACLFLNAIGYIVFSFTHSYLLLLTSRV
                     IAGVGGSSIGVAQAYIADVTTRENRSKGMGLIGAAFGLGFVFGPLIGGLLSEFGYAVT
                     GYVAAGFSSVAFLLTILYLPESLKKNQDVEHPQNKIKRKLFDLAAMKKILQKPDLAIL
                     VLLFFILTFSFANIYGTFALLGLKVYGFTDMQNGYMFGIVGLTSAIVQGGLIGRINKI
                     LSKKKILMIGSLLIMISLAMIPYAGTFLGLAMVSIVLSCGTGTFQPTVLSLISEVTSE
                     TEQGITLGMNQSLSSFARVLGPLWGGFAFEYLGYPFPFITGAIFTFVIFLLTLFYLPK
                     KIKLDY"
     gene            complement(1619..2776)
                     /locus_tag="VF870_07470"
     CDS             complement(1619..2776)
                     /locus_tag="VF870_07470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX9252065.1"
                     /translation="MKIAIVTISILSFFVFNSTASFRFDESKRIINPDSSVTISISVV
                     GDLMCHSPQFEYARVSKDSFDFSPVYRNAKKYLSNSDFTFGNLETVTAGKENGGYTGY
                     PFFNSPASYLSALKEAGFDLLVTANNHSIDRKEKGILKTIDEINSRKLHYVGTYTSQQ
                     DRDSIRIFDVKGIKIAFLAYSYGTNGNPIPKGKDYLVNLIDYNLIERDILSAKVNGAE
                     LVLVHFHFGEEYKREPVQFQKDVVNKTIALGADIIIGGHPHVLQPVNFYKTNNAKLDS
                     GFVAYSMGNFISNQRDRYKDAGMILTLKIKKDFLKNNIEINEVNYLPTWVFKGNTKYG
                     SEYLIMPSENISDTTLNLSSSELLKMNQAFDDTRYLITKYTNNIKLREQRD"
     gene            complement(2842..3033)
                     /locus_tag="VF870_07475"
     CDS             complement(2842..3033)
                     /locus_tag="VF870_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1"
                     /protein_id="HEX9252066.1"
                     /translation="MEKFNQLVQFVQSLEGDFQKFYVKEQAAAGTRVRKGLSDLRKLC
                     QEIRNDVQDVKAARKAPKP"
     gene            complement(3062..>3913)
                     /locus_tag="VF870_07480"
     CDS             complement(3062..>3913)
                     /locus_tag="VF870_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX9252067.1"
                     /translation="ANLNPVFKSLETNFTPWAFNPADRADWAEGMNIKSMAEDKEGEI
                     LFWVGCAGSFDDRYKKVSKAFATIMQKAGVDFRILGTEEKCNGDTARRLGNEYLAQMM
                     MQENVETLNNYGVKKIVTACPHCFHSLKNEYPQFGGNFEVKHHTQFIEELLSENRIQL
                     KKDSEKKKVTYHDSCYLGRYNEVYDSPRKSLHQVAGIDLVEMERNKSRGFCCGAGGGR
                     MFLEDEEGGRINEERTKEALNTNADTIASACPFCMTMMTDGVKHYEKSEEVAVKDIAE
                     IILENIN"
BASE COUNT         1253 a          777 c          573 g         1310 t
ORIGIN      
        1 taaagaaact gaatcaacaa cagatttgac aagttctact aaataaggaa tatcctttaa
       61 caaagcagaa cccgccccgc agcccacaac atttttaacc cagcatccgg catttatatc
      121 gatcatatcc ggattttctg cttccgctat tcttgcagcg ccaaccattg attctatact
      181 gctgccgtat atctgaattc caaccggacg ctcttcttcg atgatcctta atttattatg
      241 agttttagca ttcgatcgaa ccaatccttc tgagtttaca aattcagtgt aaacaacatc
      301 ggcaccaagt tccctgcaaa ccaaacggaa agaaatgtca gttacatctt ccattggtgc
      361 aagtaaaatc gcattatcta tttctatttt tcctactttg aacatatcat ctaaaaattc
      421 tagtaatcca acttaatctt ttttggtaaa taaaaaagcg ttaataaaaa tattacaaat
      481 gtaaatattg cgccagttat aaatggaaat ggatagccca aatattcaaa tgcaaatcca
      541 ccccatagcg gacccaaaac tcgtgcgaat gacgaaagtg attgattcat tccaagtgta
      601 attccctgtt cagtttctga tgttacttct gaaatcaaac ttaaaacagt aggctgaaaa
      661 gttcctgttc cgcaggataa aacgattgat accattgcca gacctaaaaa ggttccagcg
      721 taaggaatca ttgcaagtga aatcattatt aacaatgatc cgatcatcaa aatctttttc
      781 ttagataaaa ttttattaat cctgccaatc aatccgccct gaacaattgc tgaagttaat
      841 ccaacgatac caaacatata tccattttgc atatctgtaa aaccataaac ttttaatccg
      901 agcaaagcaa aagttccgta aatatttgca aaagagaatg tgagaataaa aaagagcaaa
      961 acaagaattg ccaaatcagg tttttgcaaa atctttttca ttgccgccaa atcaaataat
     1021 tttcttttta tcttattttg tggatgttca acatcttgat ttttctttag tgattcaggt
     1081 aaatataaga ttgtaagtaa aaatgctact gaagaaaaac cggcagccac ataacctgtg
     1141 actgcatacc caaactcgga taacaatcca cctataagag gaccaaaaac aaatcccaat
     1201 ccaaaagctg caccaattaa tcccatccct ttgcttctgt tttcacgagt tgtaacatcg
     1261 gcaatataag cctgggcaac gccgatgctg cttccgccaa ctcctgcaat tactcttgat
     1321 gttagaagca acaagtatga atgtgtaaaa gaaaaaacta tatatccaat cgcgttcaaa
     1381 aaaagacaag ctacaattac aggtttcctg ccgtatttat cagaaatttt ccctagtacc
     1441 gggttaaata aaaattgaac aaacgaataa attgcaatag caattccgat cgatgcttca
     1501 tcaatgtgaa gttcctttac cgaaaacgat ggaagaatcg gaatgagaat tccgaagcca
     1561 agtaaatcaa taaaaactgt taaaaatatt aataccagtg ctgatctatt tttcaaattt
     1621 aatctctttg ttctctcagt tttatattgt ttgtgtactt tgtaattaga tatcgcgtat
     1681 cgtcaaaagc ctgattcatt tttagaagtt ctgagcttga taaatttaat gttgtatctg
     1741 aaatattttc tgaaggcatt atcaagtatt cgctaccata ctttgtattt cctttaaaga
     1801 cccaggttgg aagataattt acttcattta tttcaatatt attctttaaa aagtcttttt
     1861 taatctttaa agttaatatc atccccgcat ctttatatct gtctctttga tttgaaataa
     1921 aattacccat tgagtaagca acaaaacctg aatcaagttt tgcattatta gttttataaa
     1981 aatttactgg ctgcaacaca tgcggatgcc cgccaattat aatatcagct ccaagtgcta
     2041 ttgttttatt cacaacatct ttctgaaatt gaactggttc acgtttatac tcttcaccaa
     2101 aatgaaaatg aactaagact aattccgcac catttacttt tgcagatagg atatctctct
     2161 caattaaatt gtaatcaatc aggtttacta aataatcttt gccttttgga attggatttc
     2221 catttgttcc ataagagtaa gctaggaaag caattttgat tccctttaca tcgaaaatac
     2281 gaattgaatc tctatcttgt tgtgatgtgt atgttccaac ataatgcagt tttcttgagt
     2341 taatttcatc aatcgtttta agaatgcctt tttcttttct gtcaattgaa tgattatttg
     2401 cggttacaag caagtcaaat cctgcctctt ttaatgctga taggtaagag gctggagaat
     2461 taaaaaatgg atatcctgta taaccaccat tttcttttcc agcagttact gtttctaaat
     2521 ttccaaaagt aaaatcagag tttgataaat acttttttgc gtttcggtat acaggactaa
     2581 aatcaaaact atctttgcta acccgggcgt attcaaattg cggtgagtgg cacattaaat
     2641 cgccaacaac agaaatgctg attgtaacag aactatccgg attgatgatt ctcttactct
     2701 catcaaatct aaaagatgct gtagaattaa atacaaagaa agataaaatc gatattgtaa
     2761 ctattgcaat tttcatataa tacaaaataa ggctgggcta tttctaaccc agcctttttt
     2821 aagttttgct aaagaatgct cttatggttt tggagctttt cttgcagctt taacgtcttg
     2881 aacgtcattt ctgatttcct gacataattt tctgagatcg cttaagcctt tgcgtacgcg
     2941 tgtacctgca gcagcttgtt ctttcacata aaatttttgg aaatcacctt ccaagctttg
     3001 aacgaactga acgagttgat tgaatttttc catgagaacc tcctaagtgt ttgtgaatga
     3061 attaattaat gttttccagt attatttctg caatatcttt tacagctact tcttctgatt
     3121 tttcataatg ttttaccccg tctgtcatca tagtcataca aaatggacag gctgaggcaa
     3181 ttgtatcggc atttgtattt aatgcttctt tggtgcgttc ttcattaatt ctgccgcctt
     3241 cttcatcttc taagaacatt ctaccaccac cggccccgca gcaaaaacca cgactcttat
     3301 ttctttccat ttcaactaaa tcaattccag ctacttggtg caaagatttt cttggcgaat
     3361 cataaacttc attgtatctt ccaagataac aagaatcgtg ataagttact tttttctttt
     3421 cagaatcttt ttttagctgg attctatttt ctgacaataa ctcctcaata aactgagtat
     3481 gatgcttaac ttcaaaattg ccgccaaatt gcggatactc attctttaat gaatgaaagc
     3541 aatgcggaca agcagtaaca atttttttaa caccataatt atttaatgtt tcaacgtttt
     3601 cctgcatcat catttgagca agatattcat tacctaatct tcgtgcagta tcaccgttac
     3661 atttttcttc ggtaccaaga attctaaaat caactcctgc tttttgcatt atagttgcaa
     3721 aagccttaga aacttttttg tatctatcgt caaaagatcc tgcgcagcca acccagaata
     3781 atatctcacc ctctttatct tctgccatag atttaatatt cattccctct gcccagtctg
     3841 ctctgtctgc aggattaaaa gcccagggag taaagttagt ttccaaactc ttgaacacag
     3901 gatttaagtt tgc
//
LOCUS       DASWBY010000415         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_278398, whole genome shotgun sequence.
ACCESSION   DASWBY010000415 DASWBY010000000
VERSION     DASWBY010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278398"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF870_07485"
     CDS             <1..204
                     /locus_tag="VF870_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9252068.1"
                     /translation="LKQKTMKYFENHLNENQFVRIHRSYIINLDFLQHLEQTDTESYR
                     LVLKNGKELPVSKTGLTKLKSTL"
     gene            309..800
                     /locus_tag="VF870_07490"
     CDS             309..800
                     /locus_tag="VF870_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9252069.1"
                     /translation="MITEDKIKKELIQRFGDAYKAFGLSKLMGHIIALLLFSPEPLSL
                     DEITKQLGRSKGPISQLVRRLRDKHLIRKVWMPGNNRKDFYEVEPEVFEHAFRNNLEL
                     IKNNTRIAKQLKEKVKSAKNGSLHSVNQRMVEMERFYILMEDHYNNFLTAWAKERKKL
                     YKQ"
     gene            862..1605
                     /gene="fabG"
                     /locus_tag="VF870_07495"
     CDS             862..1605
                     /gene="fabG"
                     /locus_tag="VF870_07495"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX9252070.1"
                     /translation="MKRLENKVAVVTGGAQGIGKATVKRFAEEGATVIIWDVNEEKAD
                     STIKEFKTVSDNIEFQKVDVTSLDSVTAAAAKIVEKHKKIDILINNAGITRDSSLLKM
                     TAEQWKQVIDVNLTGVFNCTKAVAPFMVENKSGKIVNTSSVVGLYGNFGQTNYVATKS
                     GIIGMTKVWARELGRKGINVNAVAPGFIATEMVETVPEKVIEMLKEKTPLGKLGDPED
                     IANAYLFLVSDEAKYITGTVLSVDGGLVL"
     gene            1602..>2249
                     /locus_tag="VF870_07500"
     CDS             1602..>2249
                     /locus_tag="VF870_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEX9252071.1"
                     /translation="MIRNAQIVSSGMYVPERVVPNSFFNELLGEDVDTWLRENLTIKE
                     RRWCNENQSTADLCVEAAKVILENADIKPAELDLLIIATDTPEYISPSTASVVQYRLG
                     AVKAGTFDVNTACAGFVTALDIASKYIRSDKKYNKVLVIGAYAMSKYLNMQDKKTVTL
                     FADGAAGVLLTSIENVSEGFLTSKLYTEGQYCDWMGIYAGGTHQPITEKVFENKDH"
BASE COUNT          823 a          346 c          447 g          633 t
ORIGIN      
        1 ctaaaacaaa aaactatgaa gtattttgaa aatcatctta atgaaaatca atttgtccgc
       61 attcatagat cttatataat aaacttagat tttcttcagc atcttgaaca aacagataca
      121 gaatcttatc gattagtttt aaagaatggt aaagaattgc ctgtaagcaa aaccggactg
      181 acgaaactaa aatcaacttt ataaagtcat taaagtctaa agtattatta attaacaaat
      241 ttcttgactt taaacaaaaa tcaagttaag tttttaacgt ttgaaaaatt tcaaaagtta
      301 aaatcagtat gataaccgaa gataaaatca aaaaagaact tattcagcgt ttcggtgatg
      361 cttataaagc atttggactg agtaagctga tgggtcacat aatcgctctc ctcctattct
      421 ctcctgaacc tttatcatta gatgaaataa caaaacagct tggaagaagc aagggaccta
      481 taagtcagct tgtaagaaga ttaagggata agcatcttat tcgtaaagtc tggatgccgg
      541 gtaataacag aaaagatttt tatgaagtgg aaccggaagt ttttgaacac gccttcagaa
      601 ataatcttga acttatcaaa aacaacacac gcattgcaaa gcagttaaaa gaaaaagtta
      661 aatcggccaa gaacggatct ctccattctg ttaatcagcg gatggtggaa atggaaaggt
      721 tttacatttt gatggaagat cattacaaca attttcttac agcctgggca aaggaaagaa
      781 aaaaactcta taaacagtaa taaaaaaatt tgaagcttgc gtttgattta tttcaaacgt
      841 taaaaatgaa ggagatttaa aatgaagaga ttagaaaata aagttgctgt ggttacaggc
      901 ggggctcagg gaatcggcaa ggcaactgtt aaacgttttg ctgaagaagg tgcgactgtt
      961 attatatggg atgtcaatga agaaaaagca gactcaacaa taaaagaatt taaaactgta
     1021 agcgataaca ttgaatttca gaaagttgat gtaaccagtt tagactctgt tacagcagcg
     1081 gcggctaaaa ttgtggagaa gcataaaaaa atagatatac ttataaacaa tgcaggaata
     1141 acacgagatt cttcccttct aaaaatgact gccgagcagt ggaaacaagt tattgatgtt
     1201 aatttaaccg gagtttttaa ctgcactaaa gctgttgcac catttatggt tgaaaataaa
     1261 tcgggtaaaa ttgttaacac atcttcggtt gttggattat acggaaattt cggacagact
     1321 aattatgttg ctactaaatc gggaataatc ggaatgacaa aagtctgggc ccgggaactc
     1381 ggcagaaaag gaattaatgt taacgcagtg gcacccggat tcatagcaac agagatggtt
     1441 gaaaccgttc ctgaaaaagt tattgagatg ttaaaagaaa aaactccgct cggtaaactc
     1501 ggcgatcctg aagacattgc aaatgcatat ttgtttttag taagcgatga agcgaaatat
     1561 ataacaggaa ctgttcttag cgttgatggc ggtttggttt tatgatcaga aatgcgcaaa
     1621 tagtttcatc aggaatgtat gttcccgaaa gagtagttcc aaattctttc ttcaatgaat
     1681 tgctaggtga agatgttgat acctggcttc gtgaaaatct tacaattaaa gaacgaagat
     1741 ggtgcaatga aaatcaatca acagccgatc tatgtgttga agctgcaaaa gtaattttag
     1801 aaaatgcaga tataaaacct gctgaacttg atctgctgat tatagctacc gatactcctg
     1861 aatatatttc cccatccact gcttctgttg tacagtacag acttggagca gttaaagcag
     1921 ggacatttga cgtaaatact gcctgtgcgg gatttgttac tgctctggat atagcatcaa
     1981 aatatattcg ctcggataaa aagtacaata aagtgcttgt tataggtgcc tacgctatga
     2041 gcaaatattt aaatatgcag gacaaaaaaa ctgtgacgct ttttgccgat ggagccgcag
     2101 gggttcttct aacatcaata gaaaatgtaa gcgaaggttt tcttacaagc aaactttata
     2161 ctgaaggaca atattgtgat tggatgggaa tttatgcagg cggaactcat caaccaataa
     2221 cagaaaaagt ttttgagaat aaagatcac
//
LOCUS       DASWBY010000416         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_52114, whole genome shotgun sequence.
ACCESSION   DASWBY010000416 DASWBY010000000
VERSION     DASWBY010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52114"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(486..1124)
                     /locus_tag="VF870_07505"
     CDS             complement(486..1124)
                     /locus_tag="VF870_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9252072.1"
                     /translation="MINVAIVEDNNTIREGLAALINGTEGYKCIGAFGDVESFLPKIG
                     ALPINVVLMDIGLPGMNGIEGVKSAVLKNPDLSILMLTVYEESEFVFDALCAGACGYL
                     VKKTPPARLLEAIKDANDGGSPMSSRIARQVITAFKEGKSIIPATQDYDLSDREISVL
                     NLLSDGYNYQEIAETLFISVDTVRHHIRNIYKKLHVHSQSEAVAKAIRKKII"
     gene            complement(1117..>1622)
                     /locus_tag="VF870_07510"
     CDS             complement(1117..>1622)
                     /locus_tag="VF870_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9252073.1"
                     /translation="ISELSRQLVDNMSDIVWVVNPSRDSLHDLILRLKDTYSELLNSM
                     GILFRTKNLEKLQDVKLPMDVKQNLYLIFKEAINNSIKHSNCKQIILESNLRNDVLEI
                     SLSDDGNGFDESKIAKGNGLKNMENRATQIKGRIKIKSSLNTGTSIRFIGKPGTINKL
                     KMIFKND"
BASE COUNT          477 a          294 c          244 g          607 t
ORIGIN      
        1 gcatcacctg ttctaacttt ttgcgagcgt aatgttttaa atcttctgcg aataatataa
       61 tctcttacag agcggtcatt aattaaaccg cggctgttaa gtaattccca aatctcatcg
      121 cttattagat cagctaatgg attagaaatt gtttttggat atatgatgtt ttccataaca
      181 tcctcctcta tattggtttg taaagttttt atcctgtcgt ttttattatt catcttttat
      241 tcttcattaa aacattctat caaattctgg gataaaagtg caaaattatt gatgtgcttg
      301 caacagcata tttatgcggt tttttagttg tggtgattta agtcttttcg tttatcgcac
      361 aattattcag agatgtaaaa caatttgtca ttccgaccga agggagaaat ctgtttggca
      421 aaataaatca gatttctctg acgctttgct ccttcgaaat gacgtcctga aattatgacc
      481 aattcctaaa ttattttttt tcttattgct tttgctactg cttcagattg tgagtgaacg
      541 tggagttttt tgtagatgtt tctgatatga tgtcgaactg tatcaacgct aataaataat
      601 gtctccgcaa tttcctgata attatatcca tctgataaaa gatttaaaac actaatttct
      661 ctatcgctta aatcataatc ttgagttgca ggaataatac ttttgccttc tttaaaagcg
      721 gtgataactt gtcgtgcaat tctggaactc ataggtgaac cgccgtcatt ggcatctttt
      781 attgcttcca gtaatcttgc tggcggagtt ttttttacaa gatagccgca agcacccgcg
      841 caaagtgcat caaaaacaaa ttcactttct tcataaacgg ttaacatcag tatgcttaga
      901 tctggatttt ttaacactgc agattttaca ccttcaattc cattcatacc cggtaaaccg
      961 atatccatta gaacaacatt aataggtaat gctccaatct ttggtaaaaa ggattcgaca
     1021 tcaccaaatg cgccaatgca cttataacct tctgttccgt ttattaatgc ggcaagacct
     1081 tctcgtattg tattgttatc ttcaactata gctacgttaa tcatttttaa atatcatttt
     1141 aagtttatta attgtgccgg gtttaccaat aaatctaatt gaagttccgg tatttaaaga
     1201 tgatttaatt ttaattcttc cttttatctg tgtggctctg ttttccatgt tttttaaccc
     1261 attgcctttg gcaattttag attcatcaaa tccattccca tcatcgctta gagaaatttc
     1321 taaaacatca tttcttaaat ttgattcaag aataatttgt ttacaattac tgtgtttaat
     1381 tgaattatta atagcttcct taaaaattaa ataaagattt tgcttaacat ccattggtag
     1441 ttttacgtcc tgaagttttt caagattttt agttctgaac aaaattccca tcgagttaag
     1501 cagttcactg taagtatctt ttaagcgaag aatcagatcg tgtaaagaat cacgactagg
     1561 gtttacaacc cacacaatat cgctcatatt atcaacaagc tgccgggata actcactaat
     1621 tt
//
LOCUS       DASWBY010000417         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_52126, whole genome shotgun sequence.
ACCESSION   DASWBY010000417 DASWBY010000000
VERSION     DASWBY010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52126"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /gene="hemW"
                     /locus_tag="VF870_07515"
     CDS             complement(<1..1072)
                     /gene="hemW"
                     /locus_tag="VF870_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX9252074.1"
                     /translation="MKNSALYIHIPFCDHKCIYCDFYSIITSDNVQSFINALKKEIEY
                     FSKLYSKDRIFTSVFFGGGTPSLMTPDYLKEILHFLKMNFNVDEKAEVTMETNPGTVD
                     KLKLKKFKDAGINRISIGIQSFDEEELKFLTRIHDRQTAIQTVYNAAEVGFDNISVDL
                     IFNLPNQTKLKWINNLETAILLPIKHLSAYSLILERGTILNKMVLDNKVIMQDDDYDA
                     DLYETTIDFLLSKDFYQYEVSNFTKPGFECVHNNAYWRYRDYLSFGTSAHSFIDGKRW
                     WNYSSLKKYISEIELNNHAIMNSEIISKEQMHDEYVMLALRSSGLDLNDYRKRFSNDW
                     IEKNYSYFELLKNEQHLSIENNW"
     gene            complement(1152..1697)
                     /locus_tag="VF870_07520"
     CDS             complement(1152..1697)
                     /locus_tag="VF870_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252075.1"
                     /translation="MKKLNTLFITIIIALTSIIINGCDPFDDIYLTLSMDTEFYVQGF
                     GPDINIGQGFCLSEFEDYNDNKDNLEEIRYISSAYLTFNATTGLQGDTLILTLYEADR
                     STVLFKYVKSTFVALEYINNPLEIVLTQEEIDNINLYLSNPKVDKCFWATLQVSNVQS
                     ASQFYSLNSKIEFLTELKIKP"
     gene            complement(1709..>2388)
                     /locus_tag="VF870_07525"
     CDS             complement(1709..>2388)
                     /locus_tag="VF870_07525"
                     /inference="COORDINATES: protein motif:HMM:NF042644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6588 family protein"
                     /protein_id="HEX9252076.1"
                     /translation="EDQKTFNPNPQLPGVETAGSTATVFGNKATYYLGSNGFYTYPTG
                     LALNAVPSGIYQFSGSMFNTELMLRFFPKIKFGDTHVGVIGFGIKHEISSYFPGIPLD
                     IAVQILYNNVDFEYDDGDPVNYTKVKSNNFAFNVHASKTFSGMFIAYGGLQYESSSMD
                     LSYYFEDINHYYPESGNQIHNLTIDGENNFRFTFGGAVKLGVFVINADINLTKFTTYT
                     TGLSLDF"
BASE COUNT          815 a          397 c          360 g          816 t
ORIGIN      
        1 tccaattatt ttcaattgat aaatgctgtt cattctttaa taattcaaaa tatgaataat
       61 ttttttcaat ccaatcatta gaaaatcttt ttctataatc gtttaaatcc aaaccggaac
      121 tacgtaaagc aagcattaca tattcatcat gcatttgttc tttagaaata atctcagaat
      181 tcataattgc gtgattattc aattcaattt ctgaaatata tttttttaga cttgaataat
      241 tccaccaacg ttttccatca ataaaagaat gtgcagaagt tccgaaactt aaataatctc
      301 tgtatctcca gtaagcattg ttgtgaacgc actcaaaacc cggttttgta aaattggaaa
      361 cttcgtattg ataaaaatct tttgaaagaa gaaaatcaat tgttgtttcg taaagatctg
      421 catcgtaatc atcatcctgc ataataactt tattatccag aaccatttta ttaagaatag
      481 taccccgctc aaggattaag ctgtaggcgg atagatgttt tatcggcagt aaaattgcag
      541 tttcaagatt gttaatccat ttcagctttg tttgattggg caggttgaag atcaaatcaa
      601 cactaatatt atcaaaacca acttcagcgg cattgtacac agtttgaata gctgtttgtc
      661 tatcgtgaat gcgggtaaga aattttaact cctcttcatc aaatgattga atcccgatac
      721 ttatccggtt tattcccgca tctttaaact tttttaattt taacttatca accgttccgg
      781 gatttgtctc cattgtaact tctgcttttt catcaacatt aaaattcatt tttaagaaat
      841 gaagaatttc tttgaggtag tcaggagtca tcaaagaagg ggttcccccg ccaaaaaata
      901 ctgaagtgaa aattcgatct ttggaataaa gtttagaaaa atattcaatc tctttcttca
      961 gagcattaat gaaagattga acattatcgg atgtaatgat ggaatagaaa tcacagtaaa
     1021 tacatttgtg atcacagaag ggaatatgaa tatatagcgc actgtttttc aaaagagaag
     1081 tttcctaata aaaaaagatg atatctttaa aaaaatatca tctttaaaac tttttaccac
     1141 caaatgtttt atcacggttt gatttttaac tcagtgagaa attcaatttt gctatttaaa
     1201 gaataaaatt gacttgctga ttgtacatta gaaacctgaa gcgttgccca aaagcattta
     1261 tcaactttcg gattgctaag atataagttg atgttatcta tttcctcttg cgtaagaact
     1321 atttccaatg gattatttat atattcaagg gccacaaaag ttgatttgac atatttaaac
     1381 agtacagttg aacgatcagc ctcataaagt gttaagataa gagtgtcccc ctgtaacccg
     1441 gttgttgcat taaatgtaag atatgccgaa gaaatatatc tgatctcttc aagattatct
     1501 ttgttatcat tatagtcttc gaattcagat aaacaaaaac cttgtccaat atttatatca
     1561 ggtccaaaac cctgaacata aaattctgtg tccatcgaaa gtgtcaggta aatatcatca
     1621 aacggatcac agccattaat tataattgac gttaatgcaa taataattgt tataaataat
     1681 gtattaagtt ttttcattga ttcactcctt aaaagtctaa tgacagacct gttgtgtagg
     1741 tcgtaaattt tgtaagatta atatcagcgt taattacaaa aacgccaagt ttaacagctc
     1801 cgccaaaagt aaatctaaaa ttattctctc catctatggt taaattatgt atttgatttc
     1861 ctgattccgg gtaataatga tttatatctt caaagtaata actaagatcc attgatgatg
     1921 attcatattg caagccgcca taagctataa acattccgct aaatgtttta cttgcatgta
     1981 cattaaatgc aaaattattc gattttactt tagtgtagtt aaccgggtca ccatcatcat
     2041 attcaaaatc aacattgtta tagagaattt ggactgcaat atccagcgga ataccaggga
     2101 aataactact gatttcgtgt ttaattccaa agcctataac acctacatga gtatcgccaa
     2161 attttatttt tggaaaaaat ctaagcatta attcagtgtt aaacatactg cctgaaaact
     2221 gatatatacc tgagggaact gcatttaatg ccaaacctgt tggataagta taaaacccgt
     2281 tactgcctaa ataataagtg gctttgtttc caaacactgt ggcagtcgat ccggcggttt
     2341 caacacctgg taattgagga tttggattaa acgttttttg atcttcgg
//
LOCUS       DASWBY010000418         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_654140, whole genome shotgun sequence.
ACCESSION   DASWBY010000418 DASWBY010000000
VERSION     DASWBY010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654140"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..958
                     /gene="rpsB"
                     /locus_tag="VF870_07530"
     CDS             122..958
                     /gene="rpsB"
                     /locus_tag="VF870_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9252077.1"
                     /translation="MKKVDLTQLIEAGAHFGHLTRRWNPKMKSYIFMEKNGIHIIDLK
                     KTQAHLTESAEELSKLVADGKKVLFVGTKKQAKNVIETEARRCGQNWVSERWLGGMLT
                     NFATIRKSVKRLSNIEKQETDGTFDKITKKERLFLTREKDKLKKVLEGVETMGKLPGA
                     LFIVDIKKEDIAVQEAHRLNIPVFAIVDTNCDPDEVDYLIPANDDAVKTVEIITQYMA
                     DAVIEGDAKLKEKKAEEVAEKERVKKEKDLVKKEELKKKSEAKAKKDAEEEIKPTEKS
                     EE"
     gene            979..1863
                     /gene="tsf"
                     /locus_tag="VF870_07535"
     CDS             979..1863
                     /gene="tsf"
                     /locus_tag="VF870_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX9252078.1"
                     /translation="MKGSELTMAITAAQVNELRQKTGAGMMDCKKALTEANGDFEKAI
                     EILRKKGASVASKRADKSANEGIVVAKVSDDHKKAALIEVNCETDFVAKSDDFIKLAN
                     EIVDVTFNSELSSAQRLLESNKSLSDKVVDVMGKVGEKIEISRVLTEKADNGLIVDYI
                     HMGSKLGVLVKFDNVSGSSDELVEIGKNIAMQVAAMNPICVYREEVPTETIEKEIDIY
                     KELARKEGKPENILEKIATGKLNKFYSENCLFEQAYIKDSTSSKTVGNLIEDYNKKNG
                     FQAKISSFKRFHLGDEKK"
     gene            1938..2666
                     /gene="pyrH"
                     /locus_tag="VF870_07540"
     CDS             1938..2666
                     /gene="pyrH"
                     /locus_tag="VF870_07540"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX9252079.1"
                     /translation="MSGVKFKRVLLKLSGESLMGEQGFGISSEVLDFFADEVKKVHDI
                     GVQLGIVIGGGNIYRGLSASKQGIDRATGDQMGMLATMINSLALQNAVEKKGIPTRLM
                     SAIKMEEIAEPYIRRRAIRHLEKGRVVILGAGTGHPYFTTDTAASLRAVEIGADIIVK
                     GTRVDGVYDSDPEKNSDALRFQNISYQDILKNNLRVMDLTAVSLCQENNLPMMVFNMD
                     MPGNLLKLVTGEPVGTFINNEISK"
     gene            2694..3248
                     /gene="frr"
                     /locus_tag="VF870_07545"
     CDS             2694..3248
                     /gene="frr"
                     /locus_tag="VF870_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX9252080.1"
                     /translation="MHQLIKDASNRMDKTLEALRSELSKVRTGKATTALLDGVKVDYY
                     GSLTPISQVGNITVLDPHTLSITPWDKSMVQPIDRAILEANLGFNPISDGTNLKIPIP
                     ALTEERRKDFVKLVKKFGEDAKVAIRNVRRDANDHLKKEQKDKKMSEDELKHSEDEVQ
                     KLTDQHIKMIDEILKHKELEIMEV"
     gene            3370..>3798
                     /locus_tag="VF870_07550"
     CDS             3370..>3798
                     /locus_tag="VF870_07550"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9252081.1"
                     /translation="MDSEPEVNKNSLIEFLSNPLLKNVIFTIGAMALFISGVIVYGVI
                     LNLREIPLSEAMTQKGFRTLNDVNIVIDRKTFALNIYEDTVFIKSYRVSLGRNLSDKK
                     KLSGDGATPIGTYKICSINNDKTYYKFLKLNYPNLEDASEA"
BASE COUNT         1454 a          497 c          763 g         1084 t
ORIGIN      
        1 gaacttgatc ttaaacttga acttttattt aataatcata ctttccgatt taccacctgt
       61 gtcccataat aaaagggatg gaaggtaaag ttgaagaaag tagaagataa acaggagcaa
      121 tatgaaaaaa gttgatctaa cgcagcttat agaagctggc gcacacttcg ggcacttaac
      181 acgccgatgg aacccaaaaa tgaaatccta catctttatg gaaaagaatg ggattcatat
      241 aatcgatctg aaaaaaactc aagctcactt aaccgaatct gccgaagaac tatctaagtt
      301 agttgcagat ggtaaaaaag tactttttgt aggaacaaaa aaacaagcta agaatgttat
      361 cgaaacagaa gcacgaagat gcgggcaaaa ttgggttagt gaaagatggc ttggtggtat
      421 gcttacaaat tttgcaacaa tcagaaaaag cgtaaagaga ttaagcaata ttgaaaagca
      481 ggaaacagat ggtacctttg ataaaataac aaagaaagaa aggctattcc ttacaagaga
      541 aaaagacaaa ctgaaaaaag ttcttgaagg tgtagaaaca atgggcaaac ttcccggcgc
      601 tttatttatt gttgacatta aaaaagaaga tattgcagtt caggaagcac acagattaaa
      661 tattccggtt tttgcaatcg ttgataccaa ttgtgatccc gatgaagttg attatttaat
      721 tcctgctaat gatgatgcgg ttaaaacagt tgaaatcatt actcaatata tggcagatgc
      781 agtgattgaa ggcgatgcaa aattgaaaga aaagaaagct gaagaagttg ctgaaaaaga
      841 aagagtaaaa aaagaaaaag atctggttaa aaaagaagaa ttgaaaaaga aatccgaagc
      901 aaaagctaaa aaagatgctg aagaagaaat aaaaccaaca gaaaaatctg aagaataata
      961 ttctgataaa ataaattttt gaaaggaagt gaattaacta tggctattac ggctgcacaa
     1021 gttaatgaat taagacaaaa aaccggcgcc ggtatgatgg actgcaagaa agcgcttact
     1081 gaagcaaatg gtgattttga aaaagctatc gaaatactaa gaaaaaaagg cgcttcggtt
     1141 gcttcaaaaa gagctgataa atctgcaaac gaaggcattg ttgttgcaaa agtttctgat
     1201 gatcataaaa aagctgcact gatagaagtt aattgtgaaa cagattttgt tgctaaaagt
     1261 gatgacttta taaaattagc taatgaaatt gtcgatgtta cttttaattc tgaattatca
     1321 agtgctcaaa gactgcttga atcgaataaa agtttaagtg ataaagttgt tgacgttatg
     1381 ggaaaagttg gtgagaagat agaaatatcc cgcgtattaa ctgaaaaagc tgataacgga
     1441 ttgatagtcg attatatcca tatgggctca aaactgggag tccttgtaaa atttgataac
     1501 gttagcggta gctctgatga attggttgaa attggtaaaa atattgctat gcaggttgct
     1561 gcaatgaatc ctatttgtgt ttacagagaa gaagtaccaa cagaaacaat tgaaaaagaa
     1621 attgatatct ataaagaatt ggcgcgtaaa gaaggtaaac cagaaaatat tcttgaaaaa
     1681 attgcaactg gtaaattgaa taaattctat tctgaaaatt gtttgtttga acaagcctat
     1741 ataaaagata gtacaagctc taaaacagtt ggaaatctga ttgaggatta taataaaaag
     1801 aatggttttc aggcaaagat aagttcattt aagcgttttc atctcggtga cgagaaaaaa
     1861 tagttttaca aaaaaggtct taataatatt aagacctttt ttttatattt aattgtaaaa
     1921 aatccaaaag gttgttaatg agcggtgtaa aatttaaaag agtactactg aaattaagcg
     1981 gtgaatcttt gatgggtgaa cagggattcg gaatttcttc agaagttttg gatttttttg
     2041 ctgatgaagt taaaaaggtt cacgatattg gagtacagct tggtatagta ataggcggag
     2101 gaaatattta tcgtggtctt agtgcaagta aacaaggtat tgatcgtgct accggtgatc
     2161 aaatgggcat gcttgctaca atgataaact ctcttgctct gcaaaatgca gttgaaaaaa
     2221 aaggtattcc aaccagactt atgagtgcaa tcaaaatgga agagattgct gaaccatata
     2281 tcagaaggag agcaatacgt catcttgaaa agggaagagt agttattttg ggtgctggaa
     2341 cgggtcatcc gtattttacc actgataccg cagcatcact gagagctgta gaaattggcg
     2401 cagatataat agttaaagga actcgtgttg atggtgttta tgattctgat ccggaaaaaa
     2461 attctgatgc actgcgtttt caaaatatat cttaccagga tattttaaaa aataatttac
     2521 gtgttatgga tctgacagca gtaagtttat gtcaggaaaa taacttacct atgatggttt
     2581 tcaatatgga tatgcccgga aatttattaa aattagttac aggggaacct gttggtacat
     2641 ttattaataa tgaaatttct aaataaaaaa ttaataataa tcagcgaggt gttatgcacc
     2701 agttaataaa agatgccagt aacagaatgg ataaaacttt ggaagcgctc agatctgagc
     2761 tttcgaaagt aagaacaggt aaagcaacca ctgccttgct tgatggtgta aaagtggatt
     2821 attacggtag tctaacccca atcagtcaag tgggcaatat aacagtcttg gatccgcata
     2881 cattatctat tactccatgg gataaatcta tggttcagcc gatagatagg gccatacttg
     2941 aagctaatct tggttttaat cctataagtg atggtactaa ccttaaaatt cctatacccg
     3001 ctttaacaga agaaagaaga aaagattttg taaaattggt aaaaaaattt ggtgaagacg
     3061 caaaagtggc aataagaaat gttagaagag atgctaatga tcatcttaaa aaagaacaaa
     3121 aagacaaaaa aatgtctgaa gacgagctta aacattctga ggatgaagtg caaaagttaa
     3181 ccgatcagca tattaaaatg atagatgaaa tcttaaagca caaagaactt gagataatgg
     3241 aagtatagat attgtcgttt atgtccaaac ccaactcgat attgagttgg gttttttttt
     3301 atctttggat caactaaggt ttatttaatt tagtattttt aattgttgta ataaataatt
     3361 ttaagtaaaa tggattccga accagaagta aataaaaata gtttaataga atttttaagt
     3421 aatcctttac ttaagaacgt tatatttact atcggagcta tggcactgtt tatttccggt
     3481 gtaattgttt acggagtaat acttaatcta agagaaattc ctctttcaga agcaatgacg
     3541 cagaagggat ttagaaccct taatgatgtt aatattgtga tagatagaaa aacattcgca
     3601 ctaaatattt atgaagacac agtttttatt aaatcatatc gtgttagttt ggggagaaat
     3661 ttaagtgata agaaaaaatt aagcggagat ggagcaacac ctataggtac ttataaaatt
     3721 tgcagtatca acaacgataa aacgtactac aaatttctga aacttaatta tccaaattta
     3781 gaagacgctt cggaagct
//
LOCUS       DASWBY010000419         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_694984, whole genome shotgun sequence.
ACCESSION   DASWBY010000419 DASWBY010000000
VERSION     DASWBY010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694984"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /gene="menE"
                     /locus_tag="VF870_07555"
     CDS             complement(<1..1415)
                     /gene="menE"
                     /locus_tag="VF870_07555"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEX9252082.1"
                     /translation="MNLESPKSLFNFSSINVLSAIIKPESTVSYSDLFFQANKIASLL
                     SKNRIKKNNYIPLLIEDNFLFIKTVIGLWYLGGVPVPLNIKLLDAEINSILGDYNFKF
                     LITDKELPSHLKKTGLEIIEIDKIAIADLELKPNSLPSLEDEAVVIFTSGSTGTPKGV
                     VHTFSSLINSVQNANQILTHQKNDRWLASLPFYHIGGFQIICRSLFYGCSIILPQSLQ
                     TDDLAKSIREFSPTHISLVSTQLERFIHHKFIPVESLRVSLIGGGFVEDELMFEADKL
                     GWKPYRVYGSSETASMITSISAGEIKTKPQSAGKALNNVEIRISNESEILIKSNSLFK
                     NYLNDEKETASKIVKGFYHTGDLGFVDDDGYLFIEARRNDLIVTGGENVNPLEVEKAL
                     LKIPFIKDACVFPKQNKTWGQIVASAIVCHDSSVDGKFLREILKQNLAGYKIPKEFYF
                     VDKLPKTSLGKLERNKIRKMF"
     gene            complement(1439..>1999)
                     /gene="menA"
                     /locus_tag="VF870_07560"
     CDS             complement(1439..>1999)
                     /gene="menA"
                     /locus_tag="VF870_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HEX9252083.1"
                     /translation="LYLVYSVGLVILWIGIFSIIAGLAYTAGPFPLAYNGLGDLFVFI
                     FFGIVGTVGTYYLHAQQFTSLAFLISLPVGALITNILIVNNYRDIEEDKSAGKNTLAV
                     LLGREFSRYEYVFFILVSFFVPFFLHFKYDFNIWIFLPYLTLPLAITLVKMIYAFNGT
                     QLNKTLELSAKFSALYGLLLSIGIIL"
BASE COUNT          706 a          325 c          308 g          660 t
ORIGIN      
        1 taaaacattt ttctgatttt attgcgttct aattttccca aacttgtttt tggtaattta
       61 tccacaaaat aaaattcttt tggaatttta tatccggcaa gattttgctt taatatttct
      121 cttaaaaatt ttccatcaac agatgaatca tgacaaacaa ttgcagatgc aactatttgt
      181 ccccaggttt tattttgttt agggaacacg caagcatctt ttataaatgg gattttaagt
      241 agtgcttttt caacttcaag aggatttaca ttttcaccac cggtaacaat caaatcattt
      301 cttcttgctt caataaataa atatccatca tcatcaacaa atcctaaatc tccggtatga
      361 taaaaccctt ttactatttt tgatgcagtt tctttttcgt cattaagata atttttaaaa
      421 agactgttag atttaattaa tatttccgat tcattcgaaa ttctgatctc gacattattt
      481 aaagctttgc cagcagattg cggttttgtc ttgatttcac cagcagaaat agatgtaatc
      541 attgaagctg tttctgagga accgtaaaca cgataaggtt tccatcccag tttatcagct
      601 tcaaacatca actcatcttc tacaaatcca ccaccgatta gagaaacacg aagagattca
      661 acaggaataa atttatgatg gataaatctt tctaattgtg ttgaaacaag tgaaatatgt
      721 gtcggcgaaa attctcttat tgattttgct aaatcatctg tctgtaaaga ttgaggaaga
      781 attattgaac atccataaaa aagtgaacga cagattattt gaaatccgcc aatgtgataa
      841 aagggaagcg atgcaagcca tctatcattt ttttgatggg ttaaaatctg atttgcattt
      901 tgaacactgt ttatcaatga tgaaaatgta tgcacaaccc cttttggcgt acctgtagat
      961 ccggaagtaa agataacaac agcctcatct tctaaagacg gtaatgaatt aggttttaat
     1021 tctaaatcag caattgcaat cttatctatt tcgataatct ctaatcctgt ttttttaaga
     1081 tgagatggca attctttatc agttattaaa aacttaaaat tataatctcc taaaatggaa
     1141 ttgatttcag catcgagtaa tttgatgttt agaggaacag gcaccccgcc taaataccaa
     1201 agcccaataa cagtcttgat aaacaaaaaa ttatcttcaa tcagtaatgg aatgtaattg
     1261 ttttttttta ttctgttttt ggaaagtaag gaagcaattt tatttgcctg aaaaaataaa
     1321 tcagagtatg agaccgttga ttcgggtttt ataattgcac taagaacgtt tatagaggaa
     1381 aaattaaata agctttttgg ggattctaaa ttcatctcat aaaatcattt tatggctttt
     1441 ataaaataat tccgattgaa agaagcaatc catatagtgc agaaaacttt gcggataatt
     1501 ccaaagtttt attaagctgt gttccgttaa atgcatatat cattttaacc aatgttattg
     1561 ctaagggaag cgttaagtat ggtagaaaaa tccagatgtt aaaatcatac ttgaagtgaa
     1621 ggaaaaatgg gacgaagaaa gaaacaagaa tgaaaaacac atattcatat cttgaaaatt
     1681 ctctgccaag caaaacagca agcgtatttt tacccgctga tttatcctct tctatatcac
     1741 ggtaattgtt tacaattaaa atatttgtta tcaatgcgcc aacaggaagt gaaataagaa
     1801 atgccaaact ggtaaattgc tgtgcgtgca aataataagt accaacagtt ccaactattc
     1861 caaaaaatat aaatacaaat aaatcaccta agccgttata agccaatgga aatgggcctg
     1921 cagtgtatgc tagtccggca attatcgaga atattccaat ccagagaatt accaatccaa
     1981 ctgaataaac aagatataa
//
LOCUS       DASWBY010000420         2560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_19801, whole genome shotgun sequence.
ACCESSION   DASWBY010000420 DASWBY010000000
VERSION     DASWBY010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19801"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..679)
                     /locus_tag="VF870_07565"
     CDS             complement(8..679)
                     /locus_tag="VF870_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9252084.1"
                     /translation="MSTIINVKNVSKSYWRDSLEIPVLNNITLDVQEGEFLSLMGPSG
                     SGKTTLLNLIAGIDKPSGGNVIIAGVDISRLGESALAKWRANNVGFIFQFYNLIPVLT
                     AFENVELPLLLTKLSKAQRKKQVETALTLVGLGDRMYHYPKQLSGGQEQRVAIARAIV
                     TDPVILVADEPTGDLDRTSADEILTLMERLNKEFQKTIIMVTHDPHAAERASHTRHLD
                     KGDLV"
     gene            complement(706..1887)
                     /locus_tag="VF870_07570"
     CDS             complement(706..1887)
                     /locus_tag="VF870_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9252085.1"
                     /translation="METKNADLSSLKIDRSKKEEINSGKKNIFITLGVVFLIIAGYFI
                     ISSFLGSSTEVKLTTAVLQKPGQSSASLTASGYVVAQRKAAIASKGTGRLIFLGVVEG
                     DQVKKNQIIGRLEDNDIVAQLEQAKANLGLQEAGLKDVQNTLNRQKDLFSKGLSSQQE
                     YDLAEANYNKTLASIEVAKAGIKAAEVALENTLIRAPFDGTVLTKNAEIGEIVAPFGG
                     STTSKTAVVTIADMKSLLVEADVSESNIEKIKPDQDCEIILDAYPEKSYQGYVFKIVP
                     TADRSKATVLVKVGFKNYDSRVLPEMSAKVNFFTQKIDEALADQKPVLVIPNTAVRTE
                     NGKSYIFKKEADKAKKIEITIGQKFGDFVEVISGLNSGDQIIDSLTDKIKDGSEVKLA
                     D"
     gene            complement(2037..>2560)
                     /locus_tag="VF870_07575"
     CDS             complement(2037..>2560)
                     /locus_tag="VF870_07575"
                     /inference="COORDINATES: protein motif:HMM:NF017396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252086.1"
                     /translation="EFSFAYWQWSDGDCSKIPDNNSSLEEIWDELAAYGGVTYFDDAS
                     ISGNYPFYYQCYTEFGYYAYDIKPFKEYIKYADGQTPFFIPKGSNPKYDPRLLKDINR
                     WASNESENFIYIYGGNDPWSSTGVCLTGKTNSIKMVLPNGSHGSRIRGFSKKDKELIY
                     SRLENWLGIQIER"
BASE COUNT          777 a          500 c          389 g          894 t
ORIGIN      
        1 attataccta aaccaaatca cctttatcaa gatgccgggt atgagaggct ctttctgcgg
       61 catgcggatc gtgggttacc attattatgg ttttttgaaa ttctttattc agccgctcca
      121 tcaatgttaa tatttcatct gcagaggttc tgtcaaggtc accagttggt tcatcggcta
      181 ccagaataac cggatcagta acgattgccc gtgcaattgc aactctttgc tcctgtcctc
      241 ctgataactg ctttggatag tgatacatcc tgtcaccaag tccaactaaa gttaaagcag
      301 tttcaacttg tttttttctt tgggctttag aaagttttgt aagcaagagt ggaagctcaa
      361 cgttttcaaa agcagttagc acagggataa gattataaaa ctgaaaaata aagccgacat
      421 tgtttgctct ccattttgct aaagcagatt cgcctagcct ggatatatct actccggcga
      481 ttataacatt accacccgat ggtttatcaa ttcccgctat cagattaagc aaagttgttt
      541 ttcctgatcc cgatggaccc attaaagaga gaaattcccc ttcctgaaca tcaagcgtga
      601 tattattcaa cactggaatt tcaagactgt ctctccaata agattttgaa acatttttta
      661 cattgataat tgttgacatt taaactccac taatacataa tctttttaat cggctaattt
      721 tacttcagaa ccatctttta ttttatcggt taaagaatca ataatttgat ctccgctatt
      781 taaaccactt ataacttcga caaaatctcc aaacttttga ccaatagtaa tttcaatttt
      841 tttagcttta tccgcttctt ttttgaaaat atatgacttc ccattttcag ttctaacagc
      901 agtattggga atcactaaaa ctggtttttg atcagccaat gcttcatcta ttttttgagt
      961 aaagaaattt accttagcgc tcatttctgg tagtactcgt gaatcataat ttttaaatcc
     1021 aacctttacc aataccgtag cttttgaacg atcggcagtc ggcacaattt taaatacata
     1081 accttgataa cttttttcag gatatgcgtc caaaattatt tcacaatcct gatctggctt
     1141 tattttttct atgtttgatt cagaaacatc tgcttctacc aaaagtgatt tcatatctgc
     1201 aattgtaaca actgcagttt ttgaagtcgt actgccacca aatggagcaa ctatctcacc
     1261 aatttctgca ttcttagtta aaactgttcc gtcaaaaggt gctcttatta atgtattttc
     1321 caaagcaact tccgccgctt taattccggc tttggctact tcaattgatg caagcgtctt
     1381 attataatta gcctcggcta aatcatattc ctgttgagaa gataaacctt tactaaatag
     1441 atctttttgt cgattcaaag tattttggac atccttcaaa ccggcttcct gaagaccaag
     1501 gttagcttta gcctgctcca gctgagcaac aatatcatta tcctcaagtc ttccaataat
     1561 ttggtttttc ttgacttgat caccttcgac aactccgaga aagatcaatc tgccggtacc
     1621 ctttgatgca attgcagctt ttctttgagc aacaacatat ccgcttgctg ttagtgatgc
     1681 agaagattgt ccgggtttct gtagtactgc agtagtcaat tttacttctg tcgaagaacc
     1741 taaaaatgaa gatattatga aatagccggc aattattaga aatacaacac ctaatgtgat
     1801 aaaaatattt tttttgcctg aatttatttc ttcttttttt gatctatcaa tttttagaga
     1861 tgataaatca gcgttttttg tttccattga aatagtttag ttatttaaag tgtaataata
     1921 aataaacgat aatcttagcc caaaagttca gtgataagtg gcacaaataa aggtttagtg
     1981 aaatataaaa aagggaatat aataattccc ttttaatgca aaatatgttt gtttttttat
     2041 cgttcgattt gaataccaag ccaattttcc aatcgagaat aaattaattc tttatctttt
     2101 ttagaaaacc ctctaatcct tgagccgtgg gaaccatttg gtaaaaccat ttttatagaa
     2161 tttgtttttc cagttaagca gactccggtt gacgaccagg ggtcatttcc gccatagatg
     2221 taaataaaat tctcactttc attcgatgcc caacgattaa tatcttttaa aagtcttgga
     2281 tcatatttag gattagaacc cttcggaata aaaaatggtg tttgtccatc agcatatttt
     2341 atatattcct taaacggttt aatatcataa gcataataac cgaactcagt atagcattga
     2401 tagtaaaatg gataattgcc actgatactt gcatcatcaa aatatgttac tcctccatac
     2461 gctgctaatt catcccaaat ttcttctaat gacgaattat tatcaggtat ttttgaacaa
     2521 tcaccatcac tccattgcca gtatgcaaat gaaaattcga
//
LOCUS       DASWBY010000421         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_542537, whole genome shotgun sequence.
ACCESSION   DASWBY010000421 DASWBY010000000
VERSION     DASWBY010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542537"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VF870_07580"
     CDS             <1..982
                     /locus_tag="VF870_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252087.1"
                     /translation="WHDYSLLMGPHPKDFIDYLCLDTYYKSNTDSISGTKIYSTFCPW
                     YGQKVSFQLNQNLIKQGFEFFYNVTAKDKGLIPKYRTRPITGYYKLIYDPSPAQVLFS
                     NDSIFIPTLIDSGSVKIVNTSDHSIRIDSIISVGSFYGYWGSFSKPGFEYPFYLFQTM
                     PGFLGDTLGIIIPPHDSIIVSFFNVDLCPFCDYEVQDYFKDTLRFVFTFMDGNVYSFS
                     KSIPISGEGHPSDVEDEEILPKEFSLSQNYPNPFNPSTKISWQSPVGSWQTLKIFDLL
                     GNEVITLVNEYRNAGSYEVDFYAHKLSSGVYYCQSRAGNFVETKKMILLR"
     gene            complement(1076..>2273)
                     /locus_tag="VF870_07585"
     CDS             complement(1076..>2273)
                     /locus_tag="VF870_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9252088.1"
                     /translation="IALSRNYVDYDSRQQDSLLNPIFTNKSKEEENTLRADLVFKLSK
                     SSELNIGGTAKLIEADYDILFPTFVTTFGDTLPITDLTTSNNYTKFGIYTNYNTVLFN
                     RLTTNLGLRSEIFTALNNKVYFSPRLSLSYALTDLTNINFSTGVYHQSPSYIWLEAFE
                     SNRDLKMIQVNQYVLGFDQRISADALLKVEAFYKDYDNYPTSTVRPYLVLANTGAGFS
                     GSDDNFASFGLEPLVDAGFGKARGIELSVQKKLSDIPYYGIFSFTYSQADYTSLDGIE
                     RPGSYDQNWLLSISGGYKFNEFWEFSSKFRFASGKPYTPYNSDGTQNVADYNTHRLKS
                     SHSLDVRVDKRWYFAGWTLITYVDIQNIYNQQNQSNVRWDPQTMSVDETSSIGILPSI
                     GISAEF"
BASE COUNT          736 a          396 c          387 g          754 t
ORIGIN      
        1 ttggcatgat tattcgcttt taatgggccc acatcccaaa gatttcatag attacttatg
       61 cttagataca tactataagt caaatacgga ctctatttct ggaacaaaaa tctactcaac
      121 tttttgtcct tggtatgggc aaaaggtttc gttccaattg aatcaaaatc tgattaaaca
      181 gggatttgag tttttttaca atgtcaccgc caaagacaaa gggttgatcc ctaaatatcg
      241 gacaagacca ataacaggtt attacaaatt aatttacgat ccatctccag ctcaagtttt
      301 attttctaat gacagtattt ttatcccaac actcattgat tcaggttctg taaagattgt
      361 taatacatca gatcattcaa taagaataga ttctataatt tctgttggtt ctttttatgg
      421 ttattggggt agttttagta agcccggttt tgaatatcca ttttatcttt ttcaaacaat
      481 gcccggattt ttgggtgata cattagggat aattattcct ccacacgatt ccataatcgt
      541 ttctttcttc aatgtagatc tttgcccctt ctgtgattat gaagtgcagg attattttaa
      601 agatacttta aggtttgttt ttacatttat ggatggtaat gtttattcat tcagcaaatc
      661 aattccaata agtggagaag gacatccatc agacgtagaa gacgaagaaa ttttaccaaa
      721 agaattttct ttatctcaaa actacccaaa cccatttaat ccaagtacaa aaataagttg
      781 gcagtctcca gtgggcagtt ggcaaacatt aaaaatattt gatttacttg gtaacgaagt
      841 tataacactt gtaaatgaat atcgaaatgc gggaagttat gaagttgatt tttatgcaca
      901 taaactttca agcggagtct attattgcca gtcgagagct ggaaattttg ttgagacaaa
      961 gaagatgatt ttgctaagat aaaatttatg gtacactgat tattatgatt gcttaagatg
     1021 ctttatgatc agttttaatc atatatgatc ataaagatca gtgtaaaatt gttatttaaa
     1081 actctgcgct aattcctatc gatggaagaa taccaatcga agaagtttca tcgacggaca
     1141 ttgtctgtgg atcccatctt acattgctct ggttttgttg attgtaaatg ttttgaatat
     1201 caacataagt aatcaacgtc cagcctgcaa agtaccagcg cttatcaact ctaacatcta
     1261 aactgtgcga agattttaat ctgtgtgtat tgtaatctgc aacgttttgt gttccatcag
     1321 aattatatgg agtataaggc ttgccgctcg caaatctgaa cttagaactg aattcccaga
     1381 actcattaaa cttatatccg ccggaaatac taagcagcca attttgatca taactgcccg
     1441 gtcgttctat tccatctaat gaagtgtaat ccgcctgact gtaagtaaaa ctgaaaattc
     1501 cgtaataagg aatatcagac aattttttct gaaccgacaa ctcaatgccg cgtgctttac
     1561 caaaaccagc atcaaccaag ggctccaaac caaaagaagc aaaattatca tcgcttcctg
     1621 aaaaacctgc gcctgtattt gcaagaacaa gataaggtct aacagtactt gttggataat
     1681 tatcataatc tttataaaaa gcttctacct tcaataaagc atcagcagaa attctttggt
     1741 caaatcccag aacatactga ttaacctgta tcatctttag atcacggtta ctttcaaaag
     1801 cttccagcca aatgtaggaa ggcgattgat gataaactcc tgtgctgaag tttatgtttg
     1861 ttagatctgt taatgcatag gaaagagata gtcttggact aaaatatact ttgttattta
     1921 acgcagtaaa tatttctgaa cgtaaaccca aattagtagt tagcctgttg aacaaaacag
     1981 tgttgtagtt tgtatagatt ccaaactttg tataattgtt gcttgtcgtt agatcagtta
     2041 tgggcaaagt atccccaaat gttgtaacaa aagttggaaa caaaatatca tagtcagcct
     2101 cgattaactt tgcagttccg ccaatgttta actcagaaga ttttgaaagt ttaaagacca
     2161 aatcagcacg taaagtattt tcttcttctt ttgatttatt ggtaaagatc ggatttagta
     2221 atgaatcctg ctgtctgcta tcgtaatcga catagttacg gcttaaggca ata
//
LOCUS       DASWBY010000422         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_278589, whole genome shotgun sequence.
ACCESSION   DASWBY010000422 DASWBY010000000
VERSION     DASWBY010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278589"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VF870_07590"
     CDS             <1..646
                     /locus_tag="VF870_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252089.1"
                     /translation="LPSYPTQDGRWGTALGVVPPQALPVELSSFSASIVGNKVKLNWR
                     TETEVNNYGFEIHRQAHTSTSLSVTAWEKIGFVNGNGNSNSPKSYFYEDKNVIGGKYS
                     YRLKQIDNDGQFEYSKVIEVDLGAPKKFELSQNYPNPFNPITKIRFNLPEAGNVKLTL
                     FNILGQELKTLVNEFKESGVYTINFDASNLNSGMYIYKLESGSFVQTRKMTLVK"
     gene            796..>1719
                     /locus_tag="VF870_07595"
     CDS             796..>1719
                     /locus_tag="VF870_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX9252090.1"
                     /translation="MEEFKDDNQKEDQQTELQSISNESNPDYDPDNDLEMQVSPVGAA
                     FIGLAGAFFLYQIVGSLITVLILGMDLESVPVNSLRLMTMAGQILFILLPALLFAKWI
                     YSDVGKIIRIRKPDLKELGLFTIGIVILTPLLQAYLYIQNYYLEVFAKNSEFINSIKS
                     FFDSLNELVEKTYSNLLSASNIFEFVLVVLVVAIVPALSEETMFRGFIQRSFELRYKK
                     YYGAIITAIFFSLYHFNPYGFLPLFVLGAFFGFAAYKSKTLLIPMFLHFLNNFSAVTL
                     YHIIGDDELIKSDASVNSNELSSYVLMFFGLV"
BASE COUNT          540 a          277 c          305 g          597 t
ORIGIN      
        1 attacccagt tatccaactc aggatggtcg ctggggaacc gcacttggag ttgttcctcc
       61 tcaagccctt ccagtagaat tatcatcctt ctctgcttca attgttggca ataaagttaa
      121 attaaactgg agaacggaaa cagaggttaa taattatggg tttgaaattc atcggcaagc
      181 tcacacttcg acttcgctca gtgtgacggc gtgggagaag attggttttg ttaatggtaa
      241 tggtaacagc aactctccaa agagttattt ttatgaggat aaaaatgtca tcggaggaaa
      301 gtattcttac agacttaaac aaattgataa cgatggacag tttgaatact caaaagtaat
      361 tgaagtggat ttaggcgcac caaagaaatt tgagttaagt cagaattatc caaatccgtt
      421 taatccaatt actaaaattc gttttaattt accagaagcc ggcaatgtaa aactaacatt
      481 gtttaacata cttggtcagg aattaaaaac acttgtaaat gaatttaaag aatcaggtgt
      541 atacacaata aactttgatg caagtaatct taacagcgga atgtacattt ataaattaga
      601 atctggttcg tttgttcaga caagaaagat gacgctagtt aaataattca aaattcaaaa
      661 tataaatttc aaaagaggct cattaattga gccttttttg ttgatataat aatactaaac
      721 gcatttcttt taaatttgag tgtcactcct tatatctgta attttaatca ttattattca
      781 actcatattt atttaatgga agaatttaaa gacgacaatc aaaaagaaga tcagcaaact
      841 gaacttcaat caatttctaa cgaaagcaat cctgattacg atcctgataa tgatttggaa
      901 atgcaggttt caccggtagg ggcagcgttt attggtctgg ccggagcatt ttttctgtat
      961 cagatagtcg gctcgctgat tacggtttta atattaggaa tggatctgga atccgttccc
     1021 gtaaacagcc ttcgtttgat gacaatggcg ggacagatac ttttcatttt gcttcctgcg
     1081 ctgttatttg caaaatggat ttattcggat gttggaaaaa ttatccgtat acgcaaacct
     1141 gatttaaaag agttgggttt atttacaatt gggattgtaa tacttactcc attactgcag
     1201 gcatacctgt atatccagaa ttattattta gaagtatttg ctaaaaactc tgaatttata
     1261 aattccataa aaagtttttt tgattcgtta aatgaattag ttgaaaaaac ttacagcaat
     1321 cttctttcgg catctaacat ttttgaattt gtattggtcg ttttagtagt tgcaattgta
     1381 cctgcgctat cagaagaaac tatgtttcgt gggtttatcc agcgtagttt tgagctgcgt
     1441 tataaaaaat attacggtgc aattatcact gcaatatttt tcagtcttta tcattttaat
     1501 ccttacggat tcctcccgtt atttgtactc ggtgctttct ttggatttgc agcatataaa
     1561 agtaaaacat tgctgatccc catgttcctg cattttctaa acaatttttc cgccgttact
     1621 ctttatcata ttattggtga tgatgagtta attaaatcag atgcttcagt aaattccaat
     1681 gaactttctt cttatgtttt aatgttcttt ggattagtc
//
LOCUS       DASWBY010000423         4533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_352810, whole genome shotgun sequence.
ACCESSION   DASWBY010000423 DASWBY010000000
VERSION     DASWBY010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352810"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..682)
                     /locus_tag="VF870_07600"
     CDS             complement(152..682)
                     /locus_tag="VF870_07600"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HEX9252091.1"
                     /translation="MEIFIAPIKFYSALLLQNLVTELSKRFTSGVHVIDLKINIDDFF
                     SADRKQYFSTQIIAEAMKLTDEFDGKIILLTDVDIFVPVLTFIFGEAQLNGKHSILSV
                     CRLHEEFYSGISNEDLLLERTIKEALHELGHCYGLRHCLDWDCVMHSSPGIEEVDIKG
                     NTFCRNCITQIEGYKH"
     gene            complement(688..2034)
                     /locus_tag="VF870_07605"
     CDS             complement(688..2034)
                     /locus_tag="VF870_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9252092.1"
                     /translation="MDKKEKILIVDDEKIVRESLYHWFEDDNYEVDTAEDGETALIKY
                     EKQKYDLLLVDMKMPGMSGLDLLSKIKAIDRDALLILITAFASVPSAITALKNGAYDY
                     VTKPVDPDELAHLIKRALEQRALKIENIPLKENIDEIIKPDNLIGESFQMKKIYELVH
                     TVARTDTTVMIRGESGTGKELVAKAIHINSNRKYFPIITVNCGALAESLLESELFGHE
                     KGSFTGAQFKRKGKFEMADRGTIFLDEIGSISLKMQIELLRVVETKQFNRVGGNELIK
                     SDFRVITATNEPLEELVKAGKFREDLYYRLNVFSIAIPPLRERRQDIPLLVNFFINKF
                     SAVMNKPVKKVSKEAMEFLTNHDWPGNVRELENAIERAMVVGKTISITVDDLPFHLSN
                     NSEYNGDEKSLASFERNYILKILNENSWNISKTAQILEIDRVTLYNKISKYSLRKA"
     gene            complement(2046..3452)
                     /locus_tag="VF870_07610"
     CDS             complement(2046..3452)
                     /locus_tag="VF870_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9252093.1"
                     /translation="MLLNRREDVHQIINTIRTEIGVEVIRVYDKQGVIAYSSDSTEIL
                     SKVNLNDEACIACHNSSVPLKSLTNQDKIRIYKNSENKRVLGVINPIQNEPDCSSADC
                     HAHSPKVNVLGVLDVVVSLEHLDSIIQTSTRKVILASGFLVVIISFFAGLFITLLVNK
                     PIKQIREGIEEVGNGNLDYKIEINSKNELGQVARRFNEMSSKLDEAYKEIKNWSETLN
                     DKVSEKSEELKNIYDQVNQIEKLASLGKLSATVAHELNNPLAGILTYSKLITKKLQTL
                     QKDNEHEQIIEFLELISHESARCGKIVKDLLIFSRSERDEFAKEDLLDIIDNSVTVLN
                     HHLDIHGIKLVKDYAETPIELYCNAYKIQQALMSLLMNSIEAMPLGGKVTIKLSLDNN
                     NVILRIIDEGTGISDKDLPHIFEPFYSTKEAANGTGLGLAVTYGIITNHKGKIEVENT
                     SNQGTTFKIVLPKNEPLA"
     gene            complement(3779..4498)
                     /locus_tag="VF870_07615"
     CDS             complement(3779..4498)
                     /locus_tag="VF870_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252094.1"
                     /translation="MIEENVYISNNYEKMKCIWMASQVVEYKLCDNQFDCENCRFDKV
                     MRNLLNEKETQNTDISNIANTISNKLRSIKFDDKIIYLKNNLIAKEICHDTFYLGINP
                     ILICFLDSVSSLSVSECRKNILTDQQVIQILGEWGSVSLSAPMNFVIYDLLDFPIETL
                     LEFQWVAIIGAVNPEVLKGRFVQDEWQSMHEKALNAIEDIKSRIPQVGNTMMDGGTQI
                     KFLHQLVGKKRYINILNSIAI"
BASE COUNT         1414 a          828 c          675 g         1616 t
ORIGIN      
        1 tggaagaata cataatcttt aaactgtttt aatctttgtt gtcaaggtct ttaatacaaa
       61 ttataattaa tatctagtca tctggttttc aacgaatcac atattagaat gtattcaaaa
      121 ataatttcgt tttcctgccc gcaatacatt attaatgttt ataaccctca atctgtgtaa
      181 tacaattacg acagaaagta tttcccttga tatctacttc ctcgatacca ggtgatgaat
      241 gcattacgca atcccaatcc aggcaatgac gcagcccgta acaatgaccc agttcgtgca
      301 gtgcttcttt aattgtgcgt tcaagtaaaa gatcttcgtt tgagattcct gaatagaatt
      361 cttcgtgcag acgacagacg gaaagaatag aatgtttacc attaagttgt gcttcaccaa
      421 atataaaagt taagaccgga acaaaaatat ccacatcggt taataagatt attttaccgt
      481 caaattcatc tgtcagcttc atagcttccg caataatctg tgttgaaaaa tattgtttcc
      541 tgtctgcaga aaagaaatca tcaatattta tttttagatc aataacatga actcctgaag
      601 tgaacctttt tgataattca gtaacaaggt tttggagcag taatgcactg taaaatttaa
      661 tgggggcaat aaaaatttcc atcaatttta agctttgcgt aagctatact tactaatttt
      721 attgtaaagt gtaactctgt cgatttcaag tatctgagcg gttttagaaa tgttccagct
      781 gttctcattt aagattttta gaatataatt tctttcaaat gaagcaagac ttttttcatc
      841 gccgttatat tcagaattat ttgaaagatg aaagggaagg tcatcaacgg taatagaaat
      901 agttttacca actaccattg ctctttctat tgcattttcc aattcacgga cgttacccgg
      961 ccaatcatgg tttgtaagaa actccattgc ttctttcgag accttcttta ccggtttatt
     1021 cataactgct gaaaatttat taatgaaaaa atttacaagc agtggaatat cctgccgtct
     1081 ttcacggagc ggagggatag caatagaaaa aacattcaat ctgtagtata aatcttctct
     1141 aaacttacca gccttaacaa gttcttcaag aggttcgttt gttgcggtaa taactctaaa
     1201 atcactcttt atcaattcgt ttccaccaac ccggttaaac tgttttgttt ctacaactct
     1261 taacaactca atttgcattt ttaaggaaat agaaccaatt tcatcaagaa aaattgtacc
     1321 ccgatccgcc atttcaaatt tcccttttct tttgaattgc gctccggtaa aagacccctt
     1381 ctcgtgtccg aacaattcac tttcgagaag tgattccgct agtgcaccgc agtttactgt
     1441 aataatcgga aagtattttc tattgctatt aatatgaata gcttttgcga ccagttcttt
     1501 tcctgttccg ctttcacctc tgatcattac tgtggtatcg gtacgggcaa cagtgtgtac
     1561 aagctcataa atttttttca tctgaaagct ctcaccgatc aaattatcag gtttaataat
     1621 ttcatcaata ttttctttca acggtatgtt ttcaatttta agcgcccgct gctcaagagc
     1681 tctttttata agatgtgcaa gttcatcagg atctacgggt tttgttacat aatcataagc
     1741 accgtttttt agtgctgtaa ttgcacttgg tactgatgca aacgctgtga tgaggattag
     1801 aagggcatcc ctatcaatcg cttttatttt agataacaaa tccaagccac tcataccggg
     1861 cattttcata tcaaccagaa ggagatcata tttctgtttt tcatatttaa ttaaagcggt
     1921 ttcaccatct tcagcagtat caacttcata attatcatct tcaaaccaat gatatagaga
     1981 ttctcttaca attttttcat catcaacaat tagtattttt tcttttttat ccataaaact
     2041 tccctttaag caagtggttc atttttaggt aaaactattt taaaagtagt accctgattt
     2101 gaagtatttt ctacttctat tttcccttta tgatttgtta tgattccgta ggttacagcc
     2161 aacccaagtc cggtaccatt ggccgcttct tttgttgaat aaaaaggttc gaagatatga
     2221 ggtaaatctt tatccgaaat cccggtgccc tcatctatta tcctgaggat aacgttatta
     2281 ttatccagtg ataatttaat tgtaactttt cctccaagcg gcatagcctc aatcgaattc
     2341 atcagaagtg acataagcgc ttgttgaatt ttatatgcat tgcagtaaag ttcaattgga
     2401 gtttctgcat aatctttaac cagcttaatg ccatgaatat caagatgatg gtttaaaaca
     2461 gtaacgctgt tgtcaattat atctaataaa tcctcttttg caaattcatc cctttcggaa
     2521 cgcgaaaaaa tcagtaagtc ctttacaatc tttccgcagc gtgcggattc atgtgaaatt
     2581 aattccagaa attcaataat ctgttcgtgt tcattatcct tttgtaaagt ttgcaacttt
     2641 tttgttatca atttgctgta ggtcaaaatt cctgcaagtg gattatttaa ttcatgagca
     2701 acagtggctg aaagttttcc gagtgaagcc agtttttcaa tttgattaac ctgatcgtaa
     2761 atatttttta gttcttctga cttttcactt actttatcat ttaaagtttc cgaccaattc
     2821 ttaatttctt tataagcttc atcaagttta gaagacatct cattaaacct tcttgcaact
     2881 tgtcccagct catttttaga attaatttca attttataat ctagatttcc attacctact
     2941 tcttcaatac cttccctgat ttgtttaatt ggtttgttca ctaaaagtgt tataaataat
     3001 cctgcaaaaa aagaaattat gacgacaaga aaacccgaag ccagaattac ttttcttgta
     3061 cttgtctgta ttattgaatc aaggtgttcc agagatacta caacatccag cacaccaaga
     3121 acattaactt ttggcgagtg ggcgtgacaa tctgcacttg aacaatcagg ttcattttga
     3181 attggattaa taaccccaag aactcgtttg ttttccgaat ttttataaat tctaatttta
     3241 tcctggttag ttaaactttt aagcggtaca gaactgttat ggcaagctat gcaggcttca
     3301 tcatttaaat taactttact taatatttct gtagaatcag aagaataagc gattacacct
     3361 tgtttgtcgt aaacacgaat tacttcaaca ccgatttcag tacgaattgt atttattatc
     3421 tgatgaacat cttcacgcct gtttaaaagc atgctgtagc gagttgactt tttaattata
     3481 tcactaatat tatatgcact ctgaaatctg gtttgggtta aataaccatc aaggtttttt
     3541 ataataaagt aggtatagat cgaaaggata atgaccagaa gacatgaaat agaaagtatg
     3601 agccgaacgc taagtctatt taataccgta attttcatag aaaacaatta actaactatt
     3661 tcaaaattag acattacttt aacgacttac aatttatttg atgttttagc ttaattgaaa
     3721 tgacatgtta ccaggtgaag tcaaagagtt tatattcatt tttagttagc aagtattttt
     3781 atatggcaat agaatttaaa atattgatat atcttttttt gcctacaagc tggtgaagaa
     3841 acttaatttg agttcctccg tccatcatag tatttccgac ctgaggtatg cgtgatttaa
     3901 tatcttcaat agcgttaaga gctttttcat gcattgactg ccattcatcc tgtacaaacc
     3961 ttccttttaa aacttcggga ttgacagcgc cgattattgc aacccactga aattctaaaa
     4021 gtgtctctat aggaaagtcc agtaaatcat aaatcacaaa attcattggt gctgaaagac
     4081 taacagaacc ccactcaccc aaaatttgaa ttacctgctg atctgttaaa atattttttc
     4141 ggcactcact aactgacaat gagcttacgg aatcaagaaa acaaattaaa ataggattta
     4201 tgccaagata aaatgtatca tgacaaatct ctttagcaat tagattgttt tttaagtaaa
     4261 tgattttatc atcaaactta atacttcgca atttgttaga aatagtattt gcaatattag
     4321 aaatatctgt attctgagtt tctttttcat tcaatagatt tctcattact ttatcaaatc
     4381 ggcaattctc gcaatcaaat tgattatcgc ataatttata ttctacaact tgcgatgcca
     4441 tccagatgca cttcatcttt tcatagttat tacttatgta aacattttct tcaatcatat
     4501 tgttcactta aaattatttg tcgtgattta ttt
//
LOCUS       DASWBY010000424         6079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_581455, whole genome shotgun sequence.
ACCESSION   DASWBY010000424 DASWBY010000000
VERSION     DASWBY010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581455"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..662)
                     /locus_tag="VF870_07620"
     CDS             complement(57..662)
                     /locus_tag="VF870_07620"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9252095.1"
                     /translation="MKSSSKNKIIEALKDQQAIFGDDLFEEKANLKKSKTTASTKEEV
                     PGDLFPGTKVVKEPIAKSLTAKSGVKGDWENSESLEQLNNLICNCTKCILHKGRNKFV
                     FGSGNPNADVMVIGEGPGAEEDKQGLPFVGRAGQLLTDILKAIKFTREEVFIGNIVKC
                     RPPDNRTPLPEEMDTCIPYLAKQIELIKPKVILCLGLTAAK"
     gene            complement(787..1077)
                     /locus_tag="VF870_07625"
     CDS             complement(787..1077)
                     /locus_tag="VF870_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX9252096.1"
                     /translation="MDITPVDLREIDKRAENVYEAISVAALRARQVNTDNKIEFNAMM
                     NTIPSSGSDDEAEDVTNPIQLKMAMDFEKKPKPHLQALNELLAGEIKYKYKK"
     gene            complement(1082..1651)
                     /gene="gmk"
                     /locus_tag="VF870_07630"
     CDS             complement(1082..1651)
                     /gene="gmk"
                     /locus_tag="VF870_07630"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX9252097.1"
                     /translation="MEKKGKIIAVSSPSGGGKTSVVKRILKDFPDIVFSVSVTTRPKR
                     SNEVDGIDYFFVSDDEFEKKIENKEFIEWERFYDYYYGTLKSFVDDNINFGKSVLFEV
                     DVKGALSLKKIYPESILIFIDPPSFEELVIRLKNRKTESESDLQKRIDRAKMELSFKK
                     DFDYIFVNDELNKVYKSVKSLIKEIINKE"
     gene            complement(1652..2530)
                     /locus_tag="VF870_07635"
     CDS             complement(1652..2530)
                     /locus_tag="VF870_07635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX9252098.1"
                     /translation="MILSMTGYGKGTASNGKWQSDVEVKTINSRYLEVFLKYPPILAN
                     KEYELRELIKSKIRRGKLNVSIQIKKNGFEDEVVSLDETRLKNYIALIKKIKKSAKIS
                     DKIKLDHILMSRDILSSSVEEINDNDFEVIKKSILLSLDSLMQMKKNEGSELEKDLKK
                     RVKSIEKYLDLIEKDSQPSVGEHFAKYKEKVKNLLEDSANINNDRLEIELAMMAERAD
                     ITEECVRLRSHIKFLLSSIYKEEDPGRKLNFLCQEMNREANTISAKTLSTAITHNSVH
                     IKEEIERIREQIQNIE"
     gene            complement(2587..4683)
                     /locus_tag="VF870_07640"
     CDS             complement(2587..4683)
                     /locus_tag="VF870_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9252099.1"
                     /translation="MFKRVFIPIIFFLLSISNLSFGQSYYDFKLSSINFFGNNFFSSS
                     ELLKNIDSKESPMWFWVFLNSFTPFGDEPVYFDSSKISVDKIALKEFYRSHGFFKTKI
                     DHEVIVDSSNQSIRLNYYIKENGFLTYGKINLIGLDKLSEYDYGRLLNESYTIDSTKR
                     FSESEVQSNISLIKRFLANNGYVFGGYDSTVVIIDTIRQKTDLSVYFHTGDKYTISET
                     VINKTGKSIEEINNNLISEIADLRPGDIYDQSKVDRSEIRLLKTELFTALDINPIIKD
                     TSKLTIPVEINSTIGSLNELSPEIKADNEFNSFNLGLGIGYTRKNFFGDARKLTLSTS
                     FRLIDIPNINFGQIFKSAADRDSTYQGVIDVKLKMEQPFLFGRPILTTTEFYLRSQTL
                     IDYTENSYGVAQKFDIEMPIYTFITLFRPFISYDVAERENVNRLVSNNINYDLSQTFK
                     SLTPGIGIELGSSKTNDILFPTTGSFLFFTPELFHSRTDISYVLRQISDQVLQAETTL
                     VGSIYFYRFQTGVSNYISLNKDQTSVFASKFRIGYSQPITGSNNPDIPAESLIPPNKT
                     FYAGGSNSVRGWDARQLIPKDSVQYFGITTETDALRGGTFWFEGSFEIRNKINQYLGY
                     AVFADYGNTWNGWKSVTIPDIAVAIGMGIRVYTPIAPFRLDFGSKFYDPFDQKMIFKK
                     NFWNNITIHFGIGEAF"
     gene            complement(4765..5379)
                     /locus_tag="VF870_07645"
     CDS             complement(4765..5379)
                     /locus_tag="VF870_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252100.1"
                     /translation="MLSNNLLKGLAFLFIVLGLTSCADKTEKIPVLDFENKSQVLDIV
                     KKHYNKDTQIAFGGLFDETGKQFIVAGFEINDPDEWGIKFVFLEKSGNEFLSKYETEL
                     LNGSFKESFVDKIKLSSIDYDLVYYNSQGYFIGSGGGEVFSYLVDLENKQVYYAHLIV
                     ESESSTSLFISENTQNKELINFFTLTFKKDYPNLKIVNDDIIVE"
     gene            complement(5366..>6079)
                     /locus_tag="VF870_07650"
     CDS             complement(5366..>6079)
                     /locus_tag="VF870_07650"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9252101.1"
                     /translation="PDPSDSPRPDRPRDNHAVPGLGPPAPPSLWRIGKVCSPIGAMAL
                     FISGVIVYGVILNLREIPLSEAITQKGFRTLSDVNIVIDRKTFTLNIYEDSVFIKSYR
                     VSLGRNLSDKKKLSGDGATPIGTYKICSISNDKTYYKFLKLNYPNLEDASEALRKSLI
                     TQKQFNQIKFEYYYEECVSPNTVLGGDIGIHGIGRLNAIFKNLPFVYNWTDGSIALSN
                     ESIDEILSVIKVGTQVVIK"
BASE COUNT         1930 a         1088 c          867 g         2194 t
ORIGIN      
        1 tcatactcaa aaacctgtcc gcgcaaactt gttaaacttt cttttttctt taataatcat
       61 ttagcagcgg taagtcctaa acacaaaata acctttggtt tgattaactc aatttgctta
      121 gctaagtaag gaatgcaggt atccatttct tctggtagcg gagtgcggtt atcaggagga
      181 cggcatttta caatattgcc gataaaaact tcttcacgtg tgaactttat tgctttaaga
      241 atatctgtta aaagctgtcc tgctctgccg acaaaaggaa gaccttgctt atcctcttct
      301 gcacctggac cttcaccaat aaccattaca tccgcatttg gattaccaga accaaataca
      361 aatttgtttc ttcctttgtg caaaatacat tttgtgcagt tacaaattaa attgtttagc
      421 tgttccagag attccgagtt ttcccaatca cctttaacac cacttttagc tgttaaggat
      481 tttgcaattg gttcctttac aactttggtt ccagggaata aatctccggg tacctcttct
      541 ttcgttgagg cagtagtttt ggattttttc agatttgctt tttcttcaaa cagatcatca
      601 ccaaaaattg cttgctgatc tttgagtgct tcaataattt tattcttgga tgatgatttc
      661 atatgacgtt caaattaaaa gatgaaaaaa gattatttaa ttagaaataa tcagccataa
      721 aaagaaatgt catccttcga ctattctaag gatgacactt ttctaaaact gatcacaaat
      781 atttaactat tttttgtatt tatacttgat ttcgccagca agtaattcgt taagtgcctg
      841 tagatgcggt tttggttttt tctcaaaatc catagccatt tttaattgaa taggattagt
      901 aacatcttca gcttcatcat cagaaccgct tgaagggatt gtgttcatca tagcattaaa
      961 ttcaattttg ttatcggtgt taacctgtct tgctcttaat gctgcaacgg aaatcgcttc
     1021 gtaaacattc tcagcgcgtt tatctatctc tcttaaatca accggggtta tatccatttt
     1081 atcactcctt atttattatt tctttaatta aactttttac gcttttataa actttattta
     1141 gttcatcatt aacaaaaata taatcaaaat ccttcttaaa gctcaattcc atttttgctc
     1201 tatcaattct tttttgaaga tcagactcac tttcagtttt tctatttttt aatcggataa
     1261 ccaattcttc aaatgaagga ggatcgataa aaataagaat actttcagga taaattttct
     1321 ttaacgataa agcaccttta acatcaactt caaaaagaac agacttacca aaatttatat
     1381 tatcatcaac aaaacttttt aatgtgccgt aataataatc gtaaaatctt tcccactcaa
     1441 taaactcttt attctctatc ttcttttcaa attcatcatc gcttacaaaa aaataatcaa
     1501 taccatcaac ttcgttagat cttttgggcc ttgtagtaac agaaacggaa aacacaatat
     1561 ccgggaaatc cttcagaatc cgtttgacaa cagaagtttt tcctcctccg ctcggcgatg
     1621 atactgcaat tatctttccc tttttctcca cttattctat gttctgaatt tgttctcgta
     1681 ttctttcaat ctcttcttta atatgaacag agttatgtgt tattgctgta gaaagagttt
     1741 tagctgaaat tgtatttgct tctctgttca tttcctggca aaggaaatta agttttcttc
     1801 caggatcttc ttccttataa atactgctta gaagaaattt aatatgactt cttagtctca
     1861 cacattcttc agtaatgtca gcgcgttcag ccatcatagc taattcgatt tcaagtctgt
     1921 cgttattaat atttgcacta tcttcaagaa gattttttac tttttcttta tactttgcaa
     1981 aatgttcacc aacacttggc tgcgaatctt tttcaatcag gtcaagatat ttttcaattg
     2041 acttaacacg cttctttaaa tctttttcta attcacttcc ttcattcttc ttcatctgca
     2101 taagtgaatc taaagaaagt aaaattgatt ttttaattac ttcaaaatca ttgtcattaa
     2161 tttcttcaac tgatgatgat aaaatatctc gactcattag aatatgatct aatttaattt
     2221 tatcagaaat cttagcagac ttttttattt ttttgattag agcaatataa tttttaagtc
     2281 tggtttcatc taaagaaaca acttcatctt caaatccgtt ttttttgatt tgaatactaa
     2341 cattaagttt ccctcttctg atcttagact taattaattc tcttaattca tactccttat
     2401 tagcaagaat aggtggatat tttagaaaaa cttccagata tctgctatta atagttttaa
     2461 cttcgacatc agattgccat ttaccatttg aggcagttcc tttgccgtaa cctgtcatgc
     2521 ttaaaatcat attattcttc tgtaaaaggg ttagaaagat agcgaattat aggctcttag
     2581 ccaagattag aacgcctcgc caataccaaa atgtattgtg atgttattcc aaaaattctt
     2641 cttaaatatc attttttgat caaatggatc ataaaatttt gacccaaaat ctaatctgaa
     2701 tggagcaatt ggagtataaa ccctgattcc catgccaata gcgactgcaa tatcaggtat
     2761 tgttacactt ttccaaccgt tccaagtatt accataatct gcaaaaacgg cataacccaa
     2821 atactggttt attttgttcc ttatttcaaa tgatccttca aaccaaaagg taccacctct
     2881 taaagcatct gtttcagtag taattccaaa atactgcaca gaatctttag gtatcaactg
     2941 tctggcatcc catcctctta cagagttact accgccggca taaaaagttt tattgggtgg
     3001 tattaaactt tctgccggta tatctggatt gttagacccg gttataggct gcgaataacc
     3061 tattctaaat tttgatgcga acactgaagt ttgatcctta tttaaagaaa tgtagttaga
     3121 aactccggtc tgaaatctgt aaaaatagat gctgccgaca agcgttgtct ctgcttgcaa
     3181 aacttgatcc gatatttgtc ttaatacgta ggaaatatct gttctggagt gaaaaagctc
     3241 tggagtgaag aaaagaaaac ttcccgtagt gggaaacaaa atatcattcg tctttgatga
     3301 acctaattca ataccaatac ccggtgttaa acttttaaat gtttgagata agtcatagtt
     3361 aatattatta gaaactaatc tgtttacatt ttccctttcg gcaacatcat aactaataaa
     3421 tggacggaaa agggtaatga atgtatagat gggcatttca atatcgaatt tttgggctac
     3481 accatatgaa ttttcagtat agtctataag ggtttgagac cttaagtaaa attctgttgt
     3541 tgttagtatc ggtcttccaa ataaaaatgg ctgttccatt tttaatttaa catctattac
     3601 accctggtaa gttgaatccc tgtcagcagc tgatttaaat atttgaccaa aatttatatt
     3661 gggaatatca atcaacctga atgatgttga aagcgtaagt tttcttgcgt ctccaaaaaa
     3721 gttttttctg gtatatccaa ttcccaaacc aagattaaaa ctattaaact cattgtcagc
     3781 ttttatttca ggagagagtt catttaatga tccaatagtt gaatttattt caacaggtat
     3841 ggtaagtttt gaagtatcct taattatagg attaatatct aaagcagtaa acaactctgt
     3901 ttttagtaat cttatttcgc ttctgtcaac tttggattga tcataaatat cacccggtct
     3961 gagatcagca atctcagaaa taagattgtt gttaatttct tctattgatt ttcctgtttt
     4021 atttattaca gtttcgctta tggtatattt atcgcctgtg tgaaaataaa cactcaggtc
     4081 ggtcttttgt ctgattgtgt ctattataac tactgtgctg tcatatcctc caaaaacata
     4141 accattattc gcaagaaatc tttttattaa tgatatattg ctttgaacct ctgattcaga
     4201 aaatcttttt gttgaatcaa ttgtgtaaga ttcatttagt aatctgccat aatcgtattc
     4261 gctaagttta tccagtccaa tcaggtttat tttaccgtac gttaaaaatc cattttcttt
     4321 tatataataa tttagtctaa ttgattgatt cgatgaatcc acgataactt catgatcaat
     4381 cttcgtttta aaaaatccat gtgaacgata aaattctttt aatgcaattt tatctactga
     4441 aatttttgag gaatcaaaat aaaccggttc atctccaaaa ggtgtaaagg aattcaaaaa
     4501 cacccagaac cacattggtg attctttgct atcaatattc tttaataatt cggacgaaga
     4561 aaaaaaatta tttccaaaaa aatttataga agatagtttg aaatcataat acgactgacc
     4621 aaatgataag tttgaaatgc tgagcaaaaa aaatatgata ggtataaaaa ctcttttaaa
     4681 cattataatg tttctaaata agaatgctga taatcgaatt tttattcaaa ttaattatga
     4741 ctgtttgttt tatcgaatca aatatcattc aactataata tcatcattca caattttaag
     4801 attaggataa tcttttttaa atgttaacgt aaaaaagttt atcaattctt tgttttgagt
     4861 attctcagaa ataaataaag aagtagaaga ttccgattcc acaataagat gagcatagta
     4921 aacctgctta ttctcaagat ctaccaagta cgaaaaaact tctccgccgc cgctgccaat
     4981 aaaatatccc tgggaattat aataaaccag atcatagtca attgatgata acttaatttt
     5041 atcaacaaaa ctttctttaa aagagccgtt aagtaattca gtttcgtatt tagaaagaaa
     5101 ttcatttccg gatttttcaa gaaaaacaaa tttaattccc cattcatcgg ggtcgttaat
     5161 ttcaaatcct gcaactataa attgtttgcc tgtctcatca aacaatcccc caaaagcaat
     5221 ctgtgtatct ttattataat gttttttaac tatatccaga acctgacttt tattttcaaa
     5281 gtcaagtact ggaatttttt ctgttttatc agcacaggaa gttaatccta gcacaataaa
     5341 aagaaaagcc aatcctttta ataaattatt tgataacaac ctgggtacct actttaatta
     5401 ctgataaaat ttcatcaatt gattcattgc ttagtgctat agatccgtct gtccaattat
     5461 aaacgaacgg aagatttttg aatattgcat ttaatctacc aataccatga atacctatat
     5521 ctccacctaa tactgtattg gggcttacac attcttcata ataatattca aactttatct
     5581 gattgaattg tttttgtgta ataagcgatt tacgtaaagc ttccgaagcg tcttctaaat
     5641 ttggataatt aagtttcaga aatttgtagt acgttttatc gttgctgata ctgcaaattt
     5701 tataagtacc tataggtgtt gctccatctc cgcttaattt ttttttatca cttaagtttc
     5761 tccccagact aacacgatat gatttaataa aaaccgagtc ttcataaata tttagtgtga
     5821 atgtttttct gtctatcaca atattaacat cgctaagggt tctaaatccc ttttgcgtaa
     5881 ttgcttcgga aaggggaatt tcccttaggt taagtattac cccataaacg attacacccg
     5941 aaataaacag tgccatagct ccgataggag aacatacttt tccaatccgc caaagtgatg
     6001 gaggagcagg tggcccaagc cctggaacag cgtgattatc ccgaggtctg tcgggccttg
     6061 gcgagtctga ggggtccgg
//
LOCUS       DASWBY010000425         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_130183, whole genome shotgun sequence.
ACCESSION   DASWBY010000425 DASWBY010000000
VERSION     DASWBY010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130183"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="VF870_07655"
     CDS             <1..1685
                     /locus_tag="VF870_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9252102.1"
                     /translation="YFRLLERKKEVPARFDFLGKKKDGSSIHTELSISSFESDNKNYV
                     LMIARDVTERIRAQRAIRESEEKYRNITENIDDFLFTFERTGLALRPIFCTSSIQKVT
                     GYTQAEFLTDSKLFLKAIHPNDYASLKPKLINLMKSRIQLSGEFEFRIINKQGNIVWV
                     RAKINLVRSGTGRILKIYGLVSDVTFRKRAEEELKKSTQNLIKLNETKDRFISIISHD
                     LRTPFSSILGFTDLLANDDELTEDERKQYVKYIQESSRSMLALVNSLLDWTRLQTGRI
                     KFEPQKLDVSKIVTDSVNALSGSAIQKGIEVLSNVNQSLALFVDKSLITQVFNNLISN
                     AIKFTHRGGFVNISSESVANSRFVKFRIKDTGVGIKNDDLPKLFSVDAKFTSEGTAGE
                     RGSGLGLSLVKEIIEKHGGTISVESEYGKGTEFVFTLPIASSNILIVDDNKTDRLLYS
                     KILKNISPEYNVEVASDGKEAIQKILSSPPALVITDHGMPVMNGYEFVKELKKMDIKV
                     KPPVIVLSSDIDRAIINDYTELGIEFVFQKPVNLSSFKLAIEKSLQKGLSED"
     gene            complement(1736..>1944)
                     /locus_tag="VF870_07660"
     CDS             complement(1736..>1944)
                     /locus_tag="VF870_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252103.1"
                     /translation="ALGYEVTHGDENFVVLFNAHPLKEAVWDLPKGKWEVLVNSEKAG
                     IKSLDTVHGKITIPTSSGFVLKKK"
BASE COUNT          680 a          325 c          344 g          595 t
ORIGIN      
        1 agtacttcag actgcttgaa aggaaaaaag aagtcccagc cagatttgat tttctcggca
       61 agaaaaaaga tggaagcagc attcatactg agctttcgat tagttctttt gaatctgata
      121 ataaaaatta tgttttaatg atagcccgtg atgttacaga aagaatacgc gctcagcgtg
      181 caataagaga atccgaagag aagtacagaa acataaccga aaacattgac gattttcttt
      241 ttacgtttga aagaaccggt ttagctttaa gacctatatt ttgcacttca tcaattcaaa
      301 aagtaactgg ttacacacaa gcagaatttt taaccgattc caaacttttc ctaaaagcca
      361 ttcaccctaa tgattatgct tctttaaagc caaaacttat taatttgatg aaaagcagaa
      421 tccagctttc aggtgagttt gaatttagaa taattaataa acagggtaat atcgtttggg
      481 taagagcaaa aattaattta gttagatccg gtacgggtag aattctgaaa atatatggat
      541 tagtcagcga tgtaacattc agaaagcgtg ctgaagaaga gttgaaaaaa tcaactcaga
      601 atcttattaa gctaaacgaa acaaaagatc gatttatttc gatcatctca catgatcttc
      661 gaactccatt cagttcaatt ttaggattta cagatcttct tgcaaacgat gatgaactta
      721 ctgaagatga acgtaagcag tatgttaagt atatccagga atcttcacga tctatgctcg
      781 cacttgtaaa ttcacttttg gattggacaa ggctgcaaac aggacgaata aaatttgagc
      841 cgcaaaaatt ggatgtttca aaaattgtca cggattctgt taacgcactt tccggctctg
      901 caattcaaaa aggaatagaa gttctgtcaa atgttaacca atctcttgcc ctttttgttg
      961 ataagagttt aataacccag gtatttaaca atcttatttc taacgctatt aaatttaccc
     1021 acaggggcgg atttgtaaat atttctagtg aatctgttgc taattcccgc tttgtaaaat
     1081 tcaggattaa ggatacagga gtaggaatta aaaatgatga tctcccaaaa cttttcagtg
     1141 tagatgctaa atttacttca gaaggcacag ccggagaaag aggaagcggg ttaggattat
     1201 cactcgttaa agaaattatt gaaaaacatg ggggaacaat ttccgtggaa agcgaatatg
     1261 gcaaagggac agaatttgtt ttcactttgc cgatcgcttc ttccaatatt ctaatagttg
     1321 atgataataa aacagatcga ttgctttatt cgaagatcct aaaaaatatt tcacctgaat
     1381 acaatgttga agttgcatct gatggaaaag aagcaattca aaaaattctt tcatcacctc
     1441 ccgccctggt aattactgac catggaatgc ctgttatgaa cggatacgaa tttgtaaaag
     1501 aactaaaaaa aatggatata aaggttaagc cgcctgtaat tgttttaagc agtgatattg
     1561 accgtgcaat tattaatgat tacacagaac taggaattga atttgttttc caaaaaccgg
     1621 ttaatcttag tagtttcaaa ttagcgatag aaaaatcgct tcaaaaagga ttaagtgagg
     1681 attaaattta atttaccttc gaggttgtat tcaaaaccag aaggttactt caatattatt
     1741 ttttctttaa cacaaaaccc gatgatgttg gaatagtaat ctttccatgt acagtgtcaa
     1801 gagattttat tccagctttt tcagaattca ctaaaacttc ccacttccct tttggcaaat
     1861 cccatacagc ttcttttagc ggatgggcat taaataaaac aacaaaattt tcatctccat
     1921 gtgttacttc ataaccaagt gcaa
//
LOCUS       DASWBY010000426         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_731182, whole genome shotgun sequence.
ACCESSION   DASWBY010000426 DASWBY010000000
VERSION     DASWBY010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731182"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1526)
                     /locus_tag="VF870_07665"
     CDS             complement(141..1526)
                     /locus_tag="VF870_07665"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9252104.1"
                     /translation="MKNSLFLRFIQISFLCLLFSIQLFPQRDSSTLDQHNLLADQIIR
                     KTLTEKPGYKLLQELCDIGPRLSGSENSLRAIHWAEEKLKSFGVDRVWLQPVMVPHWE
                     RGNVETAEIMDGKSLNILSLGESIPTPENGITANVIEVKNFTELKERQNEVKGKIVFF
                     SRPLDEGLLNTFAGYGGAVDQRFYGAVEAAKYGAVAVLIRSITTKHDNVPHTGVMGYV
                     DSLQKIPAAAIGYLDSDYLHNALVENPDLKIKLNMDCKTFPDAQSYNVVAEITGSEFP
                     NEIIVVGSHFDSWDVGCGAHDDGAGCVQSMEVLYLLKELNIKPKRTIRCVLFINEENG
                     SRGGKEYGKFVETNNENHLAAIESDRGGFTPIGFDVDSDSASLIKIKSWLPVLEKSGI
                     NWVKKGGSGVDVQYIKNTKAKLGYVPDVQRYMDVHHSANDTFESIHPRELELGAASIA
                     IMAYLISEEGL"
BASE COUNT          539 a          336 c          273 g          590 t
ORIGIN      
        1 aaaaaaacat gcgttccaaa gagaagaatt ataagcggat atccccaaag caagtcgctg
       61 atttgtgtaa gaatattttc taaagcttgc ataattttcc tttactatag ttaaatcaat
      121 aaagaatttt tgtagttctt ttataatcct tcttcgctga taagatatgc cattattgca
      181 atggaggccg caccaagttc aagctctctc ggatgaatag actcaaaagt gtcattagcg
      241 gaatgatgaa catccatata tcgctgtaca tcaggaacgt atcctaattt tgcttttgtg
      301 tttttgatat actggacatc aactccgcta ccgccttttt taacccagtt tattcctgac
      361 ttttctaaaa caggaagcca gctttttatt ttaattagac tggctgagtc ggaatcgaca
      421 tcaaatccaa ttggagtaaa tccgccgcga tcagattcta ttgctgctaa atgattttca
      481 ttatttgtct cgacaaattt tccatattct tttccgccac gagaaccatt ttcttcattg
      541 ataaacaata cgcatcggat tgttcgttta ggttttatgt taagttcttt taaaagatat
      601 aaaacttcca ttgattgaac acagccggcg ccatcatcat gcgcaccgca cccaacatcc
      661 cagctatcga aatgtgatcc aacaacaatt atttcgtttg gaaattctga acctgttatt
      721 tctgcaacta cattgtatga ttgagcatcc ggaaaagttt tacaatccat atttaatttg
      781 atttttaaat ctggattttc aaccaaagca ttatgcagat aatcagaatc taaataacct
      841 attgcggcag caggaatttt ttgcaatgaa tcaacataac ccattacacc tgtgtgaggt
      901 acattatcgt gcttcgttgt aatggatcga ataagaaccg cgacagctcc gtacttcgct
      961 gcttctacag caccataaaa cctttgatca actgcaccac catatccggc aaaggtatta
     1021 agcaaacctt catcaagagg gcgactaaag aaaactattt ttccctttac ttcgttttgt
     1081 ctttctttta attcagtaaa gttttttact tcaattacat ttgctgtgat cccattttca
     1141 ggagttggaa tgctttctcc aagcgaaaga atatttaaag attttccatc catgatttca
     1201 gctgtttcta catttcctct ttcccaatga gggaccataa caggctgcag ccaaactcta
     1261 tcaaccccaa agcttttcaa tttttcttct gcccaatgaa ttgcccgtaa agaattttct
     1321 gatccgctta atcttggtcc gatatcacaa agttcctgta ataatttgta tccgggtttt
     1381 tcggtcaaag tttttcttat tatttgatca gctaaaagat tgtgttgatc taaagttgaa
     1441 gaatcccgtt gtggaaataa ttgaattgaa aataataagc acaaaaatga aatttgtatg
     1501 aatcgtaaaa aaaggctgtt tttcaaaaag tctctcctgt tgttgaagaa ttaaaatcaa
     1561 tttgagtatt gcggaaaaaa tagcctcgat gattatcaaa acaaagatgt gttttaagaa
     1621 tttttggtgt agaatatata ctcgttaatg cgccttaaac ttatcataat aggagaatct
     1681 tttttgtttc tcaaaatatc ccccataaat tttgtagaag taaattccac ttaatatt
//
LOCUS       DASWBY010000427         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_278917, whole genome shotgun sequence.
ACCESSION   DASWBY010000427 DASWBY010000000
VERSION     DASWBY010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278917"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VF870_07670"
     CDS             complement(<1..258)
                     /locus_tag="VF870_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9252105.1"
                     /translation="MNNGQLNHRDLYRLPWTLPDNAISWLEPTAMCNLACDGCYRENL
                     KDSHLSVKEVKHHLDVFQKLRNTDCISIAGGDPLLYPDILEI"
     gene            complement(364..867)
                     /locus_tag="VF870_07675"
     CDS             complement(364..867)
                     /locus_tag="VF870_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252106.1"
                     /translation="MIQLEDISITISIYTVLQPAIAKFILNDPATLDPMDSAELSRVY
                     DSMNSVFNAGIAVEHKLNDKLSGYAAFRTDFSNANYEDIDGLSIGFTDFNIYHFTLGA
                     SMNMADTFIGVGLEYSHGGRTDFPQLFNFHTGSVEPGDIAILSQRVTSDAIYNNFNLF
                     FGVTQQL"
     gene            complement(929..3136)
                     /locus_tag="VF870_07680"
     CDS             complement(929..3136)
                     /locus_tag="VF870_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX9252107.1"
                     /translation="MNELIICSLKNKKYLLILTVLLFTGCAGHKVLLDFPPHTDNLPQ
                     IATGFYSVHPIVNGVPSDKAGTEFLGPLVYLYKDDFFIDKKYYSGIKGSFLPNYYIKT
                     KLVNSKNVYFWQDAENEDIKGDIEFYEITQNKLLASITNNKNEKEEIELRFYRHQIFG
                     DSLKVMPIAHRGLCYQPPNNYDGIFPANTFPAFESALISGYKGFELDVRVTKDKRFII
                     SHDEDLSAATTLHGYVKDKNLSDFENALVIRSAYIPEKKETAYEAFIAAPIRPLYDVL
                     YHFIDDPRLQKMVIDIKPDTDENLLTAARHDFEGLNDEQQKKILFLTRTESSAKLLKE
                     LCPFADIALEGSLGPEPINELEKFYPEAVGLPRQSHNTISFGANIILTAKSIETSQEM
                     IGKAMELSREYNYKIVMWTFAKDWKLDFLRENKFYPDFILLDIPYYQYALHQMKYASD
                     NNLTFADKDTSAEIFSNPIYKRVYNSAIKDFWFQSRTLLELTYGQGNPKHSNFTSNFA
                     PVGNWELKLGRSEIVRFSKTNTSLNEWFIFFSYLNSNSAVTKPEDNEVTTEFYRFGFG
                     RTEGYGYYWPRLSFVPFVSQSWLWTKLNDFGYTQDAAQADLPPNDAEIMNRYLDAFRF
                     GDRTLYGFKSDILSTVQLTANYETNVIYPRHLFFKWAVTSILVEAGYLGIDYVLGKFI
                     DDYPVVGPIINILVRSGYLYSYYLLRKNNMNWPFNTETPLRYEGFNLGISVVL"
     gene            complement(3311..4033)
                     /locus_tag="VF870_07685"
     CDS             complement(3311..4033)
                     /locus_tag="VF870_07685"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HEX9252108.1"
                     /translation="MKFNIRLICSGFIFSLIINYTSIAQNSSFEFWPETDIWYRINPS
                     WRLSSFIPFTIYHKSGDADLNIYLQADYAWGKTKLVHFTRLFNESRAQLMKAWLVRAG
                     IMEGWSLGERTGEYSEDMLYAEIHRRIPIGDKYLISHRLRPDLRWVGQDPEFSYRIRY
                     RLMVEKEFIDGNFSAVPYLNVELYWDSRYSTINRVRLAGGTNLSLGPTFAIEGNITYQ
                     YDSHYNTQNLYALNLILHVFFE"
BASE COUNT         1440 a          792 c          668 g         1487 t
ORIGIN      
        1 tatctctagt atatccggat aaagaagcgg atcgccgcca gcgattgaaa tacaatctgt
       61 gtttcttaat ttttgaaaaa catccagatg atgttttact tcttttactg ataaatgcga
      121 atctttaaga ttttctctat agcagccgtc gcaagctaaa ttacacattg ctgttggctc
      181 taaccaggat attgcattgt ctggtagtgt ccatggtaac ctgtataagt cccgatgatt
      241 taattgaccg ttgttcattt tctaaccttc tattatattt ttttaatatg tggtttcgta
      301 accaaatctt aattttcttt tcaatttagt ttgatgtaat agctaaaatt gttaatacaa
      361 tttttataac tgctgcgtta caccaaaaaa taaattaaag ttattgtaaa tagcatcact
      421 agtaacgcgt tgacttaata ttgcaatatc gcctggttcc actgatccag tatgaaaatt
      481 aaacaattgc gggaaatctg ttcttccccc gtgactgtac tccaatccca ctccaataaa
      541 tgtatctgcc atattcattg aggcacccag agtaaaatga taaatattaa agtctgtaaa
      601 gcctatagaa agtccatcga tatcttcgta attagcattg ctaaaatcag ttctgaaagc
      661 tgcatatccg gataatttat cattcaattt atgttcaaca gctatgcctg cattaaaaac
      721 actattcatc gaatcatata cccttgatag ttccgcacta tccattggat cgagagtagc
      781 aggatcattt agaataaatt tagcaattgc tggctgcaat acagtataga ttgaaattgt
      841 tattgaaata tcttccaatt gaatcaatta ttttattacc taatgaactc aatgacgatt
      901 cctcatttag aaactgatta aattatattt agagtacaac actaatacct aaattaaagc
      961 cttcgtatct taatggagtt tcagtattga aaggccaatt catattattc ttcctcaata
     1021 aataataaga atataaataa ccggatctga ctaaaatatt tattattggt ccgaccaccg
     1081 ggtaatcatc aatgaatttt cccaatacat agtcaatgcc cagataaccg gcttcaacta
     1141 aaattgaagt aactgcccac ttaaaaaata aatggcgtgg ataaatgacg ttagtttcat
     1201 aattagctgt taactgaaca gttgataaaa tatcggattt aaaaccgtat aatgttctat
     1261 caccaaatct gaaagcatct aagtacctgt tcataatttc tgcatcattt ggcggtaaat
     1321 ctgcttgcgc agcatcttgt gtataaccaa aatcattaag tttggtccac aaccaggatt
     1381 gagaaacgaa agggacaaaa gagagccttg gccagtaata gccataaccc tctgttctgc
     1441 caaaaccaaa tctgtaaaat tctgtagtta cttcattatc ttcgggtttg gttacagcag
     1501 aattagagtt taagtatgaa aagaaaataa accattcatt tagtgatgta ttggtcttag
     1561 agaatctaac aatttcactt cttcccaatt ttaattccca atttccaaca ggagcaaaat
     1621 tgctggtaaa attgctatgc ttaggattac cctgcccata agttaactca agtaatgtgc
     1681 gcgactgaaa ccaaaaatct tttatagcag aattataaac tcttttataa ataggattgc
     1741 tgaatatttc tgcagatgta tctttatctg caaatgttaa attattatca ctcgcatatt
     1801 tcatttgatg caaggcatac tgatagtatg gaatatcaag cagaataaaa tccggataaa
     1861 acttattctc tcttaagaag tctaatttcc agtctttagc aaacgtccac attacaattt
     1921 tgtaattgta ctctcttgat agttccattg ccttgccaat catttcctga cttgtttcaa
     1981 tagatttagc tgtgaggata atatttgcac cgaatgaaat tgtattatgt gattgacgcg
     2041 gtaaaccaac tgcttcagga taaaactttt ccaattcgtt aatgggttca ggacctaacg
     2101 aaccttcgag tgcaatatcc gcaaaaggac aaagttcctt taataattta gctgaacttt
     2161 cagtacgagt aagaaataaa atctttttct gctgctcatc attaagccct tcaaaatcat
     2221 gcctggcagc cgtaagtaga ttttcatcag tatcaggttt aatatcaatt accatttttt
     2281 gtaatctggg atcatctata aaatggtaaa gtacatcgta tagaggtctt atcggcgcgg
     2341 caataaatgc ctcgtaagct gtttcttttt tttccggaat ataagcactc ctgatcacta
     2401 gtgcattttc aaaatcggac aaatttttat cttttacata accgtgtaac gtagtggcag
     2461 cacttaagtc ttcatcatga cttattataa atcttttatc tttggtaact ctgacatcta
     2521 actcaaatcc tttatatcct gaaattaaag ctgattcgaa agcaggaaaa gtatttgctg
     2581 gaaatatacc atcataatta tttggtggtt gatagcataa acctctgtgt gcaattggca
     2641 ttactttcag gctatcaccg aatatctgat gacgataaaa tcttaattca atttcttctt
     2701 tttcattttt attgttggta atagatgcaa gtagtttatt ctgagtaatt tcgtaaaact
     2761 ctatatcacc tttgatgtcc tcgttttctg catcctgcca gaaataaaca ttttttgaat
     2821 taaccagttt tgtctttatg taataatttg gaaggaatga acctttaata ccactataat
     2881 atttcttgtc tataaaaaaa tcatctttat ataaataaac tagtggacct aaaaattctg
     2941 tacctgcttt atctgaaggt acaccgttta cgataggatg aacagaatag aagcctgtgg
     3001 caatctgtgg taaattgtct gtatgcggcg gaaagtccaa cagaacttta tgacctgcac
     3061 aaccggtaaa tagaagtacg gttaggatta gcaggtactt cttatttttt aatgaacaaa
     3121 ttattaattc attcatatca ttaaaactca accttttgaa gtttataaat tatattattc
     3181 ataatattaa aagaacacct tctgccaagt gatcaaaaca atagcgaagt cagcaccaat
     3241 tctaattact ttacctgtac tgtatgctaa ctttatggaa tcctttaatc ttcacgagat
     3301 taaataatct ttattcaaag aacacgtgca atattaggtt taacgcatag agattttgcg
     3361 tattataatg tgaatcatat tgataagtta tgtttccctc tattgcgaag gttggaccca
     3421 acgacaaatt agtacctcca gctaaacgga cccggttgat cgtagagtat cgcgaatccc
     3481 aataaagttc tacatttaaa tatgggacgg ctgaaaaatt accatctata aactcttttt
     3541 caaccataag tctataacgt atcctataag agaattctgg atcctgtcca acccagcgta
     3601 gatcaggtct gaggcggtgg gatatcaagt acttgtctcc aatcggtatt cttctatgaa
     3661 tttctgcata tagcatatcc tcagaatatt ctcctgtcct ttcacccaaa ctccatcctt
     3721 ccattattcc agcacgcact aaccatgctt tcattagctg tgctctgctt tcattaaata
     3781 atctggtgaa atgcacaagt ttggtttttc cccaagcata gtctgcctgc aaatagatat
     3841 tcaaatctgc atccccgctc ttatgataaa tagtaaaagg aataaacgat gaaagccgcc
     3901 aggatggatt tattctgtac catatatcag tttcaggcca aaattcaaaa gaagaatttt
     3961 gtgctatgga agtatagttt atgattaaac taaatataaa acctgagcaa atcagtcgta
     4021 tgttaaattt caaactcaat gctttttaaa tttattctat tctaaaagta taaatttacg
     4081 gccaccaaaa ttgatgaccg aaaattttga taattcgaat ttaaatttga taaataaatt
     4141 acttaaacca cttagtagtt aaacattaag ttttacaaaa ggacttagga gaaattcgta
     4201 taacttaatt actgatctca aaatcagata gtttataaaa aaagagaata ctgggttata
     4261 tattttcaat cttcttttat actgtttttt tatttccagg attatcaatc ttaagtttaa
     4321 gtttatttct cttttcactc tcgctatgcg caagagactg tgcaagtttt gtaacgattc
     4381 ccacaaa
//
LOCUS       DASWBY010000428         4054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_167889, whole genome shotgun sequence.
ACCESSION   DASWBY010000428 DASWBY010000000
VERSION     DASWBY010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167889"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VF870_07690"
     CDS             complement(<1..664)
                     /locus_tag="VF870_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252109.1"
                     /translation="MITIDYIIVFVFLAGMFYVGSVFYKWVGSSDDFYLAGRQLTPFI
                     LAAVLAATNVNLYSFVGQAGIAYKEGIPIIWQTWTGNMAMVIAGLFVIPIFRRLRIRT
                     IPEFLEKRYSKGVRTFVAFLWIVRLTFWLGVVLYTAVIAAQTISGFNSFTAWVLIFSV
                     VVILYTILGGMWSVAFTDVIQFVLMLGGSLILLPLVMSAVGWWPGLTAKLPEGALTLV
                     KNT"
     gene            complement(693..830)
                     /locus_tag="VF870_07695"
     CDS             complement(693..830)
                     /locus_tag="VF870_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252110.1"
                     /translation="MYFDWATWSIWLVGFIIMVIWILIPLKEFKKLVQQKRADEKSKT
                     L"
     gene            complement(854..2272)
                     /locus_tag="VF870_07700"
     CDS             complement(854..2272)
                     /locus_tag="VF870_07700"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9252111.1"
                     /translation="MKTNFSRRNFIKLSALSTAALGLPSVTPALSKDKILDRVEKLTL
                     APKQKGKSVIGLTVPPIKQVKVAFIGLGNRGIEHLKLVDSLCPDKAAITAICDVQQTK
                     VDAGVAELKKSGHGQNPKVYSGTTDIWKEMIKRDDIDLVIISTPWEDHVPMSILSMQN
                     NKHAAVEVPAAYTLDDMWKLVDTAEETQRNCMMMENVCYGDEELWLLNMAQQGVLGTL
                     TYAECAYIHNLRELLFSKDYYYNMWRIRHHDTRDGNLYPTHGLGPVAQYMDVNRGDSF
                     ESIVSMSSLQASLDEYSKQIESTNEFYNHTGYKHGDMNNSLIRTKKGRMILVQHDVVT
                     PRPYNRINALAGTKGYHEGYPSRLSLVEDKEKGHEWLSEDNYNTYKEKYKHPLWNKLK
                     AGIEKYGGHGGMDFVMIYRLIDSLNNGWFLDQDVYDAASWSVIIPLSALSIELGNVPL
                     KFPDFTRGRSNETRELGILKNY"
     gene            complement(2356..3945)
                     /locus_tag="VF870_07705"
     CDS             complement(2356..3945)
                     /locus_tag="VF870_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEX9252112.1"
                     /translation="MLRLIIIFLCFSISSIAQEASGEYYFESILSTGEGWWGGVVVDG
                     PLMPFGKYEYKHNLYGDVKENQGSPVLISNSGGYIWCDEPFKFEFNNDSLKVSSNLSH
                     INSGKSGSSLRDAYLYVSQNFFPPQGKIPEALLFTSPQYNTWIELTYNQNEVDILNYA
                     QAIIDNGFPPGVIMIDDNWQEDYGTWNFKAAKFNDPKKMVNKLHELGFKVMLWVCPFV
                     SADSPNYRYLEQENAFLMEDNEEKRPAIIRWWNGASALLDFTNPFAEKWFIDQLKSLM
                     ETYDIDGFKFDAGDPNFYTGEIVSNQNVTPNKHSELFAEIGLEFPLNEYRACWKMGGQ
                     PLAQRLRDKRHDWEDLKLLIPGIIAQGLMGYQFTCPDMIGGGEYGSFLNLKTIDEELI
                     VRSAQCNALMPMMQFSVAPWRILSKENIEICREMADLHRKMGDEILKLAKESAVSGEP
                     IVRNMEYVFPHEGYSEIKDQFMLGNDILVAPVLEKNTNSREIIFPSGKWSGDDESIVS
                     GPCKIKVEVPINRLPWYRRIN"
BASE COUNT         1217 a          868 c          662 g         1307 t
ORIGIN      
        1 ctgtattttt aacaagagta agagcgcctt ccggcagctt tgctgttaaa cccggccacc
       61 atcctacagc actcattact aacggaagaa gaataagcga accgccaagc attaaaacga
      121 attgaattac atcagtaaat gcaactgacc acataccacc aagaattgta taaaggatta
      181 caacgacaga aaatatcagt acccaggctg taaatgaatt aaatccggat attgtctgag
      241 cagcgataac tgcagtataa agcactacac ccagccagaa agtaagtcgg acaatccaaa
      301 gaaaagccac aaatgtccga acacctttgc tgtagcgttt ttcaagaaac tccggaatag
      361 tacgtatacg aagtcttcta aagatcggga taacaaaaag tccggcaatg accattgcca
      421 tattaccggt ccaggtttgc cagattatag gaatgccctc tttgtatgct attcctgcct
      481 gaccgacaaa actataaagg tttacattag tcgcggcgag tacagcggca agtataaatg
      541 gagtaagttg tcttccggca agataaaagt catcagaact tccgacccat ttataaaaga
      601 ctgaaccgac ataaaacatt ccagcaagaa acacgaagac gattatatag tctatagtta
      661 tcaaaatttt tctccggtat aatattatta agttataatg ttttggattt ttcgtccgct
      721 cgtttttgtt gcactaactt tttaaattct ttaagaggaa ttaatatcca aataaccata
      781 atgataaatc caacaagcca gattgaccat gttgcccaat caaaatacat ttttatcttc
      841 tttcagatta tgattaataa ttttttaata tacccaattc tcttgtctca ttgcttcttc
      901 ctcttgtaaa gtcaggaaac ttcaatggta cgtttccaag ttctatcgat aatgcagaaa
      961 gcggtattat aaccgaccac gaagcggcat catagacatc ctgatccagg aaccacccgt
     1021 tgttaagact gtcgatcaat ctataaatca tcacgaagtc cattcctcca tgaccgccgt
     1081 atttctcaat acctgcttta agcttattcc atagtggatg tttatatttt tctttatatg
     1141 tattataatt atcctcactc agccactcat gacctttctc tttatcttca acaagtgaga
     1201 gtctgctggg ataaccttca tgatatcctt tcgtaccagc caatgcatta attctgttgt
     1261 atggccttgg agttacaaca tcatgctgaa caagtatcat tctgcctttt ttagttctga
     1321 ttaatgaatt gttcatatca ccgtgtttat aaccggtatg attataaaat tcatttgtgc
     1381 tttcaatttg ttttgaatat tcatctaaag aagcctgaag cgaactcata gatacaatag
     1441 attcaaatga atcgcctcta ttaacatcca tatattgagc caccggtccg aggccgtgtg
     1501 tcggataaag atttccatca cgggtatcat gatggcggat tcgccacata ttgtaataat
     1561 agtctttgct aaatagaagt tcacggagat tatgaatgta tgcacactct gcatatgtga
     1621 gtgtacctaa tactccctgc tgcgccatat ttaacaacca aagttcttca tctccgtaac
     1681 aaacattttc catcatcata caattgcgct gtgtttcttc tgctgtgtct accagtttcc
     1741 acatatcatc taatgtataa gctgccggta cctcaaccgc tgcgtgttta ttgttctgca
     1801 ttgaaagaat actcattgga acgtgatctt cccagggagt agagataatt actaaatcaa
     1861 tgtcatctct tttaatcatt tctttccaaa tgtccgttgt gcctgaatat actttcggat
     1921 tctgaccgtg accggatttt ttaagttctg cgactccggc atcgactttg gtctgctgca
     1981 catcgcaaat agcagtgata gcagctttat caggacaaag tgaatcaacc aattttaaat
     2041 gttcgatgcc tctgttaccg agccctataa atgcaacttt aacttgtttg attggaggaa
     2101 ctgtcagacc gattactgat ttgccttttt gttttggagc aagagtaagt ttttcaactc
     2161 tgtccagaat tttatcttta cttaatgccg gagttacact tggcaggccc aatgcagcag
     2221 tgcttaatgc tgataacttt ataaaatttc ttcgcgaaaa attagttttc attttatctc
     2281 cagtattttt aattaaactt atctttttaa aagtacaatt tatttacaat ttcttaatta
     2341 cttaaaactg ttttttcaat ttattcttct gtaccagggg agtctgttga tcggaacttc
     2401 cacttttatt ttacacggac ctgatacaat actttcatcg tctcccgacc atttccccga
     2461 tggaaaaata atttccctgc tgttagtatt tttttcaaga accggggcta ccagaatatc
     2521 atttcccagc ataaactgat cttttatttc cgaatatcct tcatggggaa agacatattc
     2581 catattcctg actattggct caccggaaac agcactttcc tttgcaagtt ttagaatttc
     2641 atcacccatt tttctgtgca aatcggccat ctctctgcat atttcaatat tttctttgct
     2701 caaaatccgc cagggtgcaa ctgaaaactg catcataggc attaatgcat tgcattgcgc
     2761 tgatcgtaca atcaattctt cgtctatagt tttaagattt aagaatgatc cgtattcacc
     2821 gcctccaatc atatcaggac atgtaaactg ataacccatt agtccttgtg caataattcc
     2881 gggaattaga agtttgagat cctcccaatc gtgtctttta tccctgaggc gctgtgccaa
     2941 aggctggccg cccattttcc agcaagccct atactcgttc aacggaaact caagaccaat
     3001 ttctgcaaaa agttcagaat gcttgttagg agtaacgttc tggtttgata ctatttctcc
     3061 ggtataaaaa ttcggatcgc ctgcatcaaa cttaaatcca tcaatatcat atgtttccat
     3121 aagtgatttt aactgatcaa taaaccattt ttcagcgaaa ggatttgtaa aatcaagaag
     3181 ggcgcttgca ccgttccacc acctgattat agcaggtctt ttttcctcat tatcttccat
     3241 caaaaaagca ttctcctgtt ccagatatct gtaatttgga gaatcagcac tcacaaacgg
     3301 acatacccac agcattactt tgaatccgag ttcatgcagt ttattaacca tttttttcgg
     3361 atcattaaat tttgccgctt taaaattcca tgttccatag tcctcctgcc agttatcatc
     3421 aatcataatt acacctggtg gaaaaccgtt atcaataatg gcttgtgcgt agtttaaaat
     3481 atctacttca ttttgattgt aagtaagttc aatccaggta ttgtattgag gcgatgtaaa
     3541 taataaagct tcaggtattt taccctgagg aggaaaaaag ttttgagaaa catataaata
     3601 ggcatctcgt aaagaactgc cggacttgcc tgaatttata tgacttaaat tcgaggatac
     3661 ttttaagctg tcattattaa attcaaattt gaacggttca tcgcaccaga tataaccacc
     3721 tgaatttgaa attaagactg gtgagccctg attttcctta acatcaccgt aaaggttatg
     3781 cttgtattca tactttccga aaggcattag tggaccgtct acaactacac cgccccacca
     3841 tccttcacca gtgctgagaa tgctttcaaa ataatattca ccgctggctt cctgagctat
     3901 cgatgaaata gaaaagcaga gaaaaataat tatcaaccgt aacacattaa accaatttta
     3961 tcattgaaca ttttcatcat cttgataatt tttgatgtat cacatccagt aatggtgttc
     4021 tgccatcttt taaatagtct cccatcagag gcca
//
LOCUS       DASWBY010000429         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_52556, whole genome shotgun sequence.
ACCESSION   DASWBY010000429 DASWBY010000000
VERSION     DASWBY010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52556"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VF870_07710"
     CDS             <1..1005
                     /locus_tag="VF870_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252113.1"
                     /translation="TSIGNVFIEDLKIVPDVNHSGYPDILVSGISPNIHMIEGLTGIN
                     IWSNSTGGNILGKDVLGDLNSDGIPEVGSASLNDLIHVYNSVTGNIIFDYAFGGGTNS
                     FAAEHIVDLDDVDGNFSNEFAACSRDGRVILFSGGDDIVPVELFSFTANTFENNVQLF
                     WMTATETNNSGFSIERKMENQDWITIGYINGHGTTTVRQSYNFIDDYVSKGTYAYRLK
                     QINYDGTYEYSNQIEVEVGSPLNFTLQQNYPNPFNPATKIKYSIPNKSKVSLKIYDML
                     GGEVAELVSGEIEAGYYEMNFDGSSLSSGVYFYRLQAGDPSKGSGQSFVETKKMILLK
                     "
     gene            1291..>1594
                     /locus_tag="VF870_07715"
     CDS             1291..>1594
                     /locus_tag="VF870_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252114.1"
                     /translation="MKTFFQILLFILITVQISFSQILEDTKNPRKTSEVLPIRLPHPT
                     HTHMLGKLTGSLHPSFYDRKDEWQKIIDSTWGAGEPLSGKLETFDLYKNFARSYNAT"
BASE COUNT          520 a          244 c          310 g          520 t
ORIGIN      
        1 acaagtattg gcaatgtttt tattgaagat ttaaagattg ttcctgacgt gaatcattct
       61 ggttatcctg atattctagt cagcgggatc agtccgaaca ttcatatgat agagggatta
      121 actggaataa atatttggtc aaattcaact ggtgggaata ttttaggtaa agatgtactt
      181 ggcgatttaa attcggatgg tattccggaa gtgggcagtg catcattaaa cgatttaatt
      241 catgtttata atagcgttac tggaaatatc atttttgatt atgcatttgg aggaggtaca
      301 aattcatttg cagcagagca cattgttgac cttgatgatg ttgatggaaa ttttagtaat
      361 gaatttgccg catgttcacg tgatggaaga gtaatattgt tttcaggtgg agatgatata
      421 gtacctgttg aactttttag ttttacggca aatacttttg aaaataatgt gcaattattc
      481 tggatgactg ctactgaaac aaataattcc ggctttagta ttgaaaggaa aatggagaat
      541 caggattgga taacaatagg gtatataaat ggccatggaa ctacaactgt aaggcaatcc
      601 tataacttta ttgatgatta tgtcagtaag gggacttatg catacagatt gaaacaaatt
      661 aattatgacg gaacatatga atattcaaat cagattgaag ttgaagtagg ttccccatta
      721 aattttactc ttcaacagaa ttatccaaac ccatttaatc cggctacaaa gataaaatat
      781 tccatcccaa acaaatcaaa agttagtttg aagatatatg atatgcttgg tggtgaagtt
      841 gcagaattag tatctggaga aattgaagcc ggatattatg agatgaactt tgatggatcg
      901 tcactgtcca gcggcgttta tttctacaga ttgcaggcag gagatccttc aaaaggctca
      961 ggacaaagtt ttgttgagac taagaaaatg atacttctta aataatttac aacctgccta
     1021 gacggcaagg ctggtgtaga atgtacaatg tacaattaag gcgattcaga aggatcgcct
     1081 tttttattgc tgtttatata tacttgctaa tctgcaatcg attcttcttg acaataacaa
     1141 aaattagtat agatttatca ctaacaaaat taaactactc cgagagggct aaactttaga
     1201 gccattttat tgttaagtcc tgagatgaca tcccgatact ctcccgaagc agaaatgttg
     1261 atagacattt ttattccatt ggaggttacc atgaaaacat tttttcaaat tctgcttttt
     1321 atcttaatta cagttcaaat atccttttct cagattttgg aggatacaaa aaatcctcga
     1381 aagacaagtg aggtcctacc aattaggcta ccacatccaa cccatacaca tatgttgggt
     1441 aaattgactg gttcattaca tccttctttt tatgatcgca aagatgaatg gcaaaagatt
     1501 attgattcaa cttggggcgc tggtgaacca ctttctggga agcttgaaac ttttgatctt
     1561 tataaaaact ttgctcgttc ttataatgca acat
//
LOCUS       DASWBY010000430         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_279059, whole genome shotgun sequence.
ACCESSION   DASWBY010000430 DASWBY010000000
VERSION     DASWBY010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279059"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VF870_07720"
     CDS             <1..347
                     /locus_tag="VF870_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX9252115.1"
                     /translation="TAEIYKVVESMEEIADSLAVGQNWNNDVRIILFVVLKNDLQLDN
                     NLIEKIKTKIRTTATPRHVPALIYQIKEVPHTISGKKVELAVTKILNGEKVENRDALA
                     NPESLNQFNNLF"
     gene            444..944
                     /locus_tag="VF870_07725"
     CDS             444..944
                     /locus_tag="VF870_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX9252116.1"
                     /translation="MYDKSIELLNKAIADELHAVHQYMYFHFLLDDMGYDLLAGLFKR
                     TAIDEMMHVEKLAERILFLKGEVEMKVGGEVEKIKDVAKMLEKAAGMEQESAKEYNVW
                     ANECSANADSATKQIFEGLVSEEERHYDQYDTEADNLKKFGDRYLALQSIERSKNLSA
                     AGRPVE"
     gene            1148..1612
                     /locus_tag="VF870_07730"
     CDS             1148..1612
                     /locus_tag="VF870_07730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9252117.1"
                     /translation="MQVKPIQDYYPDDVAHCYGCGRLNEHGHHIKSYWDGDETLARFN
                     PKEYHTAIPGFVYGGLIASLIECHGTGTAALASYRAENREFDTMPPLRFVTASLKVDY
                     LKPTPIGVELELRGKVIELKGKKVISEITVTANGVITARGEVVAVKMSENML"
     gene            1682..2095
                     /locus_tag="VF870_07735"
     CDS             1682..2095
                     /locus_tag="VF870_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEX9252118.1"
                     /translation="MESKQINIAVVTDDGITISQHFGRAKYYEVLFVENNKVVKRERR
                     EKMGHHNFPNSEHHHHDHNGEHGMDESSHNKHVSMAEAIKDCNIVISRGMGYGAFQSL
                     SKLNINTIITDIKNIDDAVQSVIDGTIVNHTEKLH"
     gene            complement(2144..2722)
                     /locus_tag="VF870_07740"
     CDS             complement(2144..2722)
                     /locus_tag="VF870_07740"
                     /inference="COORDINATES: protein motif:HMM:NF021485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HEX9252119.1"
                     /translation="MKYILILGLFIFVPLFIIAQNCGQKDNSKQKCNGDKGNCVQVID
                     SIKYDLSSTEKDGLIYMRLEEKLARDVYVTLGKTFAQKMFVNIPQSEQRHMDAVKVLL
                     DKYEIPDPVINNEIGLFSNSEFQKLYNDLVKKGQISFKDAMLVGKEIEELDIKDLNER
                     LEETDNPDIKSVYKNLKHGSENHLKAFVKHLD"
BASE COUNT         1097 a          456 c          540 g          962 t
ORIGIN      
        1 gtacagcaga aatttacaaa gtagttgaat caatggaaga aattgcggat agtttagctg
       61 tggggcaaaa ctggaataat gatgtaagaa taattttgtt tgttgtatta aaaaatgatc
      121 tccaacttga caataactta atcgaaaaga taaaaactaa aatcagaaca actgcgacac
      181 caagacacgt gccggcacta atttatcaaa taaaagaagt tccgcacaca ataagcggta
      241 aaaaagtgga gcttgcagta acaaaaattc taaatggtga aaaagttgaa aaccgcgatg
      301 ctcttgctaa tcctgaatca ctaaatcaat tcaataatct tttttaaaat acacttctct
      361 ttgatctcaa tctcagcaac taagtcagat attccttaat tttgtactaa caataagtta
      421 aatatttatt aaggagaaat aatatgtatg acaaaagtat agagctttta aacaaagcta
      481 ttgcagatga acttcatgca gttcatcaat atatgtattt ccatttttta ttagatgata
      541 tgggctacga tttattagca ggactattta aacgcactgc tattgatgaa atgatgcacg
      601 tagaaaaatt agctgagaga atcttgttct taaaagggga agtcgagatg aaagtcggtg
      661 gtgaagttga gaaaataaaa gatgttgcaa agatgcttga aaaagctgcc ggtatggaac
      721 aggaaagtgc aaaagagtat aatgtctggg ctaatgagtg ttctgcaaat gcggattcag
      781 cgactaaaca gatatttgaa ggattggtct ctgaagaaga aagacattat gatcagtatg
      841 acactgaagc agacaatctt aaaaagtttg gcgacaggta tcttgccctt caatcaattg
      901 aacgaagcaa aaatctttcc gctgccggaa gacctgttga ataaataatt ttatattttg
      961 atttttcata taccgcatta gataattttg gtgcggtatt ttttttaagt tactggttat
     1021 caaccagaag atttaatgga tgaaattaaa ttgaataaaa agaaaaaatt tgtaatttca
     1081 caaaaagata gattctttct gaaggctcta aataaattga ataacttaaa ttattttcgg
     1141 atataaaatg caagtaaaac caattcaaga ttactacccc gatgatgttg cacattgcta
     1201 cggctgcggt agattaaatg aacatgggca tcatataaaa agttattggg atggagatga
     1261 aacactggca aggtttaacc caaaagaata ccatactgca attcctggat ttgtttatgg
     1321 cggactgatt gcatctttaa ttgagtgtca cggaaccgga actgcagctt tagcatctta
     1381 cagggcagag aatagagaat ttgatacaat gccgccttta cgttttgtta cagcctctct
     1441 taaagtggat tatttaaaac caactccgat tggcgttgaa cttgaattaa gaggcaaagt
     1501 aatcgagcta aaagggaaga aagtaatctc tgaaattaca gtaactgcaa atggagttat
     1561 tactgcgcgc ggggaagtgg ttgcagttaa aatgtcggag aatatgttat gaacattttt
     1621 taatacaatt aatttagata aagttaactt actaattcat caaatattta aatggagaaa
     1681 aatggagagc aaacaaatta atatagcagt agttactgat gacggaataa ctatcagtca
     1741 gcattttggc agagcaaagt attatgaagt attgtttgtg gaaaacaata aagtagtcaa
     1801 gagagaacga cgtgaaaaaa tgggtcatca taattttcca aattcagaac atcatcatca
     1861 tgatcacaat ggtgaacatg gaatggatga atcttcacac aacaaacatg tgagcatggc
     1921 agaagctatt aaagattgca acatagttat ttctcgtgga atgggttatg gagcttttca
     1981 gagtctatct aaacttaaca taaatacaat aataacggat atcaagaata ttgacgatgc
     2041 agttcagtca gttatagatg gcaccatagt aaaccatact gaaaaattac attagcagta
     2101 aaatcgttaa agtatgaaat agagatatat actttaaatt attttaatct aaatgtttaa
     2161 caaaagcttt aagatgattt tctgaaccat gtttaagatt tttataaaca gattttatat
     2221 cagggttatc tgtttcttcc aatctttcat ttagatcttt gatatcaagt tcttcaattt
     2281 cttttccgac aagcatagca tctttaaatg atatttgtcc ttttttcaca agatcattat
     2341 aaagcttttg aaattctgaa ttactgaata aaccaatctc gttattaatt acaggatctg
     2401 gaatttcata tttgtcaagt aaaactttta ctgcatccat atgacgttgt tcgctttgcg
     2461 gaatgtttac aaacattttt tgtgcaaatg tttttccaag agttacgtaa acgtcacgag
     2521 caagcttttc ctccaatcgc atataaatca gtccatcttt ttctgttgat gacagatcat
     2581 acttgattga atcaataacc tgcacacaat ttcctttatc accattacat ttttgttttg
     2641 aattgtcttt ttgcccacaa ttctgagcta ttataaaaag agggacaaaa ataaataagc
     2701 caagtattaa aatatatttc ataatatttt ttctgtttga ttatctagcg ctgacaaaag
     2761 tctgcgtaat taattgttta aattattgtc acaacttaca agctgaaatt tacaactcca
     2821 ctaacaatat cctaaaataa catgtctatt tttgaatatc atctattaat aaaaagaaga
     2881 cggtctttgc agaccgtctt ccgggaagga gaaggaggac acactacgaa ctgtaccatc
     2941 gggggcgcta tatagtttat tcggcaatct cggatgttgc acatccgatt aaatttttat
     3001 taaatgctgc aagatgacta gttgaagcat cttcaagttt agtatacatt tttat
//
LOCUS       DASWBY010000431         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_243687, whole genome shotgun sequence.
ACCESSION   DASWBY010000431 DASWBY010000000
VERSION     DASWBY010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243687"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VF870_07745"
     CDS             complement(<1..1616)
                     /locus_tag="VF870_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9252120.1"
                     /translation="MFNKIGFRLIFVVAFTTLIIISVFAYFNVKSQSGSLISEVERHA
                     NQLGETVISSTRFDMMYNQRDRIQQTINSIGTQPQIRDVRIINKVGEIIYSSDSTNIN
                     KMVDKRAESCYACHAEDQPLEQISITERTRIFRVHPDSNRVIGIITPIYNEKSCWKAE
                     CHAHPKDQKVLGVLDVSISLAEVDKAIVNSEWKIVGFSVIAILSVGFLIAFFIRRWVS
                     RPVNDLLNGTLQVSQGNLNYTIKEMGDDEIGKLGTSFNNMTKKLAEARMQLFQSDKMA
                     SLGRLAAGVAHEINNPLTGVLTYSSFLLKRTKDNPEFQEDLKVIVRETIRSREIVKSL
                     LDFARQSVPKKGSANINDIIDKAVGVIDSQLTLNKVKIVKQTETNLPNLTVDANQIQQ
                     VIINLLVNASDAIGKAGGVITLQTKQISLAPVGIAQIKKATCPKRHSLIDNDFRIDGL
                     PSLKVKVISAGKEGIVHLDPVYGKHRNNFDVGFKIEKDAKFVCPECNNSIVELDRLCP
                     ICKSPVLSFEISGQGIYEVCSSENSNWEKW"
     gene            complement(1654..2796)
                     /locus_tag="VF870_07750"
     CDS             complement(1654..2796)
                     /locus_tag="VF870_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9252121.1"
                     /translation="MKKIFVLLTVLFFAAAIHAQQSPGKDHANLQINCKTCHTCDVPT
                     KSEPCLVLCPREKMTTVYQKPEQTPELIVLDQISDRYTPVYFSHRIHAQMSNMSGGCE
                     GCHHFNTSGPILKCSNCHESSRKREDVSVPDLKGAYHRQCMDCHREWSHDTGCNTCHT
                     LKKDLKDVKKVDIQKKYAGKEHPVVLEPTKLVYETKSDKGKFVTFYHNDHTKKFGLNC
                     TTCHKQESCTKCHDVNRTDKNKNQKVVTKKTFEDQHKNCISCHSKTEDCSKCHSDKTL
                     EPFDHAKKTGWPLNKYHINLNCVKCHGSKLPYKKVDNRCSSCHQNWDSENFKHSVTGL
                     QLDENHSAFSCEDCHIDKNFAVKPSCNNCHDDFSFPKQKPGKMVGK"
BASE COUNT          843 a          553 c          448 g         1040 t
ORIGIN      
        1 tcccattttt cccaattgct attttccgaa gaacaaactt cataaatgcc ttgtccgctt
       61 atttcaaaag ataaaactgg tgatttgcaa atcggacata gtctgtccag ttctacaata
      121 gaattattac attctggaca aacaaatttt gcatcttttt caattttaaa accaacatca
      181 aaattattcc gatgttttcc ataaaccgga tcaaggtgta cgataccttc tttaccagcg
      241 gagataactt taacctttag tgatggcaac ccatctattc taaaatcatt atctatcaaa
      301 ctatggcgtt tagggcaagt agctttttta atctgagcaa ttccaacagg agctaatgat
      361 atttgtttgg tctgaagtgt aattactcca ccagcttttc caattgcatc agaagcattt
      421 acaagaaggt ttataattac ctgctgaatt tgatttgcat ctactgttaa atttggcaga
      481 tttgtttcag tttgttttac aattttaact ttgtttaaag ttaactgact gtctataaca
      541 ccaacagctt tatcaattat gtcgttaatg tttgcactac cttttttcgg aacagactgc
      601 cttgcaaaat ccagcaaact ttttacaatt tctctgcttc tgatcgtttc ccttacaatt
      661 acttttaaat cctcctgaaa ctccggatta tcttttgttc tttttaacaa aaaactactg
      721 taagttaaaa caccggtaag cggattattt atttcatgag caactccggc agctaatctt
      781 cctaacgaag ccattttatc agattgaaat agctgcattc gagcttcggc caatttcttg
      841 gtcatattat taaaagatgt accaagtttt ccaatctcat catcacccat ttctttaatt
      901 gtgtaattaa gattaccctg actgacttgc aaagttccgt ttagtaaatc attaaccggt
      961 ctggaaaccc aacgacgaat aaaaaatgca atcaggaaac ctaccgatag aattgcaatt
     1021 actgagaagc cgactatttt ccattcgctg tttactattg ctttatcaac ttctgcaagt
     1081 gagatagaaa catcaagtac accaagaact ttttgatctt tcggatgtgc atggcattct
     1141 gctttccagc acgatttttc attataaatg ggtgtgataa ttccaattac acgatttgaa
     1201 tcaggatgaa ccctgaatat tcgtgtacgt tcagttatag atatctgttc aagcggctga
     1261 tcttcagcgt gacatgcgta gcagctctct gcccttttat caaccatttt attaatgttt
     1321 gtcgaatctg aagaataaat tatttcgcca accttattaa ttattcttac atctctaatt
     1381 tgaggttgag tgccaattga attaatggtc tgttgaattc ggtcccgctg gttgtacatc
     1441 atatcaaatc ttgtacttga tattacagtt tcaccaagct gattcgcatg acgttcaact
     1501 tcagaaatta agctgccgct ttgtgattta acattaaagt atgcaaacac acttatgatg
     1561 atcaaagttg tgaaagctac aacaaatatt aaacggaatc cgattttatt aaacataatt
     1621 actaagacaa atctctctga tgaattttaa aaattacttt cctaccattt tccccggttt
     1681 ttgtttaggg aatgaaaaat catcatgaca attattgcaa cttggtttaa ctgcaaagtt
     1741 cttatctata tggcaatctt cgcagctaaa agctgaatga ttttcatcaa gctgcaaacc
     1801 tgttacagaa tgtttaaagt tttcactatc ccagttttga tgacaacttg aacatctatt
     1861 atcaactttt ttgtaaggaa gttttgagcc atggcatttt acacaattaa gattaatatg
     1921 atacttattt aatggccacc cggttttctt tgcgtgatca aagggttcaa gtgttttatc
     1981 actatgacac ttactgcagt cttctgtttt actatgacag ctaatgcaat ttttatgctg
     2041 atcttcaaag gtttttttag ttacaacttt ctgattttta ttcttatcag tcctattaac
     2101 atcatgacat ttagtgcaac tttcctgttt atggcaagta gtgcagttta atccaaattt
     2161 ctttgtatga tcattatgat aaaatgtgac aaattttcct ttatcggatt tagtttcata
     2221 aactaattta gttggttcaa gcactaccgg atgttcttta ccagcatatt ttttttggat
     2281 gtcaactttt ttaacatcct tcaaatcttt ctttaatgta tggcaggtat tgcaccctgt
     2341 atcatggctc cattcacgat gacaatccat acattgtctg tggtatgcac ctttcaaatc
     2401 cggaacacta acatcttcgc gttttcttga tgactcgtgg caattactgc attttaatat
     2461 gggtcccgaa gtattgaaat gatgacatcc ttcacatccc ccgctcatat ttgacatttg
     2521 agcgtgtatc ctgtgagaga agtaaactgg agtatatcta tcacttattt gatctaaaac
     2581 aatcagttcc ggagtttgtt ctggtttttg ataaaccgtt gtcatttttt ctcttggaca
     2641 aagaacaagg caaggttcac tttttgtcgg aacatcgcat gtatgacatg ttttacaatt
     2701 aatttgaaga tttgcatgat ccttaccagg actctgctgt gcatgtattg cagctgcaaa
     2761 aaacaatacc gttagtagta caaatatctt tttcattatt ttcctcctct tattgtgtat
     2821 ttggattgag gagcagcttg tttacccggc aaactgataa ccggaaaatt tattacgata
     2881 aatc
//
LOCUS       DASWBY010000432         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_130290, whole genome shotgun sequence.
ACCESSION   DASWBY010000432 DASWBY010000000
VERSION     DASWBY010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130290"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VF870_07755"
     CDS             complement(<1..351)
                     /locus_tag="VF870_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9252122.1"
                     /translation="MTKPQIWVAAFLLLFIILFMIGRLTKEEETMKDFSGMNNSAMGQ
                     QSNEEMTGENLVMSFGCVNCHGGNLAGSNMAPSLKGLKQYWSSRDDLINYLRNPNSFM
                     DSDRFKKYRAEYPNG"
     gene            complement(453..1184)
                     /locus_tag="VF870_07760"
     CDS             complement(453..1184)
                     /locus_tag="VF870_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX9252123.1"
                     /translation="MEKKNNLFLPAAILGISFIIGVVFFTNAWKSSQRANQTINVTGS
                     AKIEIVSDLGFLRGTISVQSSTSESAFAELNRQKPILVSYLDKKGFTKDKIEFFTINS
                     YPIYQMSEQGYQTGKVIGYVYNQRIEIQSSDVNKIKELSLDITSLIEKGVSFNVEQPE
                     YHYTKMADLKIEIQAAAAKDAMIRAQKIAEATDRDLGPMRSARMGVLQITPKFSNAIS
                     DYGINDLSSIEKEIVGVVNASFEIE"
     gene            1312..>2041
                     /locus_tag="VF870_07765"
     CDS             1312..>2041
                     /locus_tag="VF870_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX9252124.1"
                     /translation="MNIPNKLINEKSPYLLQHAYNPVNWFPWSEEAFEKAKAEDKPIF
                     LSIGYSTCHWCHVMEKESFEDEEVAKLMNEAFVSIKVDREERPDIDGIYMSVCQMITG
                     GGGWPLTIVMTPDKKPFFTGTYFPKHNRFNRIGMMDLIPRLKEVWAIKKEDVLKSANE
                     IALSLNKQNLISDNNDVDESILDKAYEELNKTYDETNGGFGNAPKFPSPHNLLLLLRY
                     WKRKNEPKALEMVEKTLTEMRRGGI"
BASE COUNT          670 a          358 c          331 g          682 t
ORIGIN      
        1 accgtttgga tattctgctc tgtatttttt aaacctgtca gaatccataa atgaatttgg
       61 attgcgaaga taatttataa gatcatctct gctgctccaa tattgcttta atccttttaa
      121 tgaaggcgcc atatttgatc ctgcaagatt accaccgtga caatttacac atccaaaaga
      181 catcacaaga ttttctcctg tcatctcttc attcgattgc tgacccatcg ctgagttgtt
      241 catcccggaa aaatctttca tcgtttcttc ttctttagtt aaacgtccaa tcataaaaag
      301 gattatgaat aaaagtaaaa aagctgctac ccaaatttgt ggttttgtca tatatctgta
      361 attgttttgt taacgaaaaa tttttatttg tttataatcc aacgcctttt cagaattgtc
      421 atctcttgaa aggctaaata cgataagcac tattactcta tctcaaaaga agcatttaca
      481 actccgacta tttctttttc aatagagcta agatcattta ttccataatc agaaattgca
      541 tttgaaaatt ttggtgtaat ttgaagaacg cccattcttg cacttctcat tggtccaaga
      601 tctctatccg ttgcttcagc tattttttgc gctcttatca tagcatcttt tgctgccgca
      661 gcctgtattt caatttttag atctgccatt ttagtgtaat gatattccgg ttgttcaaca
      721 ttaaaactga ctcccttttc aatgagtgaa gttatatcaa gtgaaagctc tttaatttta
      781 tttacatcag aagattgaat ttctatccgc tgattataaa cgtacccaat aacttttcct
      841 gtttgatatc cttgctcgct catttgataa attggataac tattaatagt aaaaaactca
      901 attttatctt ttgtaaaacc ttttttatca agataagaaa ctagaatggg tttttgccga
      961 ttcaattctg caaaagcact ttcagatgtt gaagactgaa cagagattgt tccgcgaaga
     1021 aatcctaaat cagatacaat ttctatttta gctgaaccgg ttacatttat agtttgatta
     1081 gctctttggc ttgatttcca tgcattggta aaaaatacga caccaataat aaaactaatt
     1141 cccaagatag ccgcaggaag aaagaggtta ttcttttttt ccatattatt ttatgaattt
     1201 tttagttatt aattgtgtta aaacgtgtgg caacttatca aaaaagagta attttaacaa
     1261 cagaatttga ttggtatctt ataatataaa ttaaaaataa cacataattt tatgaatatt
     1321 cctaacaaac tgataaacga aaaaagtcct tatttactgc agcatgcgta caatcccgta
     1381 aattggttcc cgtggagcga ggaggctttt gaaaaggcaa aagctgaaga taaaccaatt
     1441 tttctttcaa tcggatactc gacctgccat tggtgccacg taatggaaaa ggaatccttt
     1501 gaagatgaag aagttgcaaa gttgatgaat gaagcttttg tatccataaa agttgaccgc
     1561 gaagaacgac ctgatattga tgggatttat atgtccgttt gccaaatgat tacaggcgga
     1621 ggaggctggc cgcttacaat tgtaatgact cctgataaaa agccattctt cacgggaaca
     1681 tattttccca aacataatcg atttaataga attggaatga tggaccttat tccaagatta
     1741 aaagaagttt gggcaattaa gaaagaagat gttcttaaat ctgcgaatga gattgcctta
     1801 tctctaaaca aacagaattt aatttcagat aataatgatg ttgatgaatc aattttagat
     1861 aaagcttatg aggaattaaa caaaacatac gacgaaacaa atggcggatt tggcaatgct
     1921 ccaaaatttc cctcaccgca taatttatta cttcttcttc gctactggaa aagaaagaat
     1981 gaacccaaag cgcttgaaat ggttgaaaaa accctaaccg aaatgaggcg cggcggcatt
     2041 t
//
LOCUS       DASWBY010000433         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_471380, whole genome shotgun sequence.
ACCESSION   DASWBY010000433 DASWBY010000000
VERSION     DASWBY010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471380"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2557
                     /locus_tag="VF870_07770"
     CDS             <1..2557
                     /locus_tag="VF870_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252125.1"
                     /translation="TLDQSTSTDRFYRTIFGGKVGESSDDPLTIIYAYLTDPRRSTPR
                     WWKEGIAVFLETWMAGGIGRAMGGYDEMVFRTLVQEEKSIYDIISLETEGTQTDFQVG
                     ANSYLYGTRFMSYLSLKYGPEKLVQWTSRYEGSDAYFVSEFNNIYNLSLSEAWNQWII
                     FENDFQKKNLEKIRLNPVTAYRTLSTRPLGALSRAFYDSTSSTIYAAVDFPGQVAQIV
                     AYDTRTGNAEKIHDVKGAALYYVTSLAFDPTSQTLFYTTDNNDWRSVYSINIKSGDEK
                     LLLKEERIGDLVFNQADKSLWGIRHFNGQSTLVRMPHPYSEWNQIYTWPFGQDMYNID
                     ISRDGKNVTGALAEINGQQLLIRMNIDSIMSGNFSYDTLFNFENTIPANFVFSDDGKY
                     LFGTSYYSGVSNIYRYDLDKKDMEIMSNSETGFFRPVPISMDSILAFKFTSEGFQPII
                     LANKPELNVNAIDFLGQKIVEQHPIVEDWIAPPPSSINIDSVMTDPKAYSVFTDFGIV
                     SLYPVVQGYKDYFSYGLRFNLSDPIGFQNIHLTASYSPYSQLSVNERYHLDFAYSYLN
                     WKFESTYNRADFYDLFGPTKSSRKGYSFGLNYHKNVIYDSPRIMDYTIFTNYYGNLER
                     LPDYQNVSVNYDNYFNAGIDFNYQDLRASLGSVDREKGYRLKLSSNNNFVNKTIYPHI
                     NTNFDFGFALPINHSSVWLRSSVGYSYGNRLEPLANFYFGGFGNNYVDNLDEKRYREY
                     YSFPGVELNHIGGTNFGKLMFEWNLPPIRFSSIGFSSFFLNYARTSLFSTVITTNIDS
                     DNYRRSLANIGAQVDFKFIMFFHLKMTFSFGYAMAFEKDQSRSDELMFSLKIL"
     gene            2562..3563
                     /locus_tag="VF870_07775"
     CDS             2562..3563
                     /locus_tag="VF870_07775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX9252126.1"
                     /translation="MIKILISLLPVFIFLLILIYLDSYKLVKITNVILLIFMGFVAAI
                     LSYVINDFLLANLSVDITTYAKYASPVIEEILKASFIIYTIRKKKIGFMVDAAIYGFA
                     IGAGFSFIENIYYLNVIESSNIFLWIIRGFGTAVMHGGTTAIFSILTKNFFDRSNKSK
                     FKYYLPGLVAAILIHSFFNHLLFPAITITILQLIFLPILIIFTFHKSEQALKDWMEIG
                     LNNDVQILEQIDNGIFSESHAGQYLLSLQSRYSGTILADMLCLIKIHSELSIRAKGVI
                     LMRKVGLPVIIEDEVKEKLEELRYLEKSIGPTGRLAIAPIFNISTKDLWQLYMLGLK"
     gene            complement(3671..4036)
                     /locus_tag="VF870_07780"
     CDS             complement(3671..4036)
                     /locus_tag="VF870_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252127.1"
                     /translation="MLSSQLRGHWLRKHDTHSKHNRLELPIFIAKSTNGKLKLTIDRE
                     ELPNYLNLSLKKCYQLTEGFYLPETKIINSPRVKIDYKNLASEYKRLINNGTVLNRAA
                     LARKLNVSRAWVTKVFRLG"
BASE COUNT         1448 a          676 c          787 g         1624 t
ORIGIN      
        1 aaccctagat caatcaacct ccactgacag attttaccgg acaatattcg gtggtaaagt
       61 tggtgaatct tcagatgatc cgttaacaat aatttatgcc tatctcacgg atccacggag
      121 aagcacgccc cgctggtgga aggaaggtat agctgtattc cttgaaacat ggatggccgg
      181 tggaattgga agagcaatgg ggggatatga tgagatggtt tttagaacac tcgttcaaga
      241 agaaaaatca atttatgata ttataagtct tgaaacggaa ggaactcaaa ccgatttcca
      301 ggttggtgca aattcgtatt tgtacggaac gcgatttatg agttatcttt ctcttaaata
      361 tggtccagag aaattagtac aatggacatc acggtatgaa ggtagtgatg cttattttgt
      421 atccgaattt aataatattt ataatttgtc tttatcagaa gcctggaatc aatggataat
      481 ttttgaaaat gacttccaga agaaaaactt agaaaaaatc cgattaaacc ctgttactgc
      541 ttatcgcaca ttatctacga gaccattggg tgcactatcc agagcatttt atgattctac
      601 tagctctaca atttatgctg ctgttgattt tccgggacag gttgcacaaa tagtggcata
      661 tgatacaagg actggtaacg cagaaaaaat tcacgatgtt aaaggggctg ctttatacta
      721 tgttacttca ttagcgttcg atccaacttc acagacttta ttttatacaa cagataataa
      781 tgattggcga agcgtttatt caattaatat aaaatccgga gatgaaaaac ttcttttaaa
      841 ggaagaaaga attggggatc tggtttttaa tcaggcagat aaatcactat ggggaataag
      901 gcatttcaat ggtcaatcaa ctttagttag aatgcctcat ccatattctg aatggaacca
      961 gatttatact tggccttttg gacaggatat gtataacatt gatatttcca gagatggcaa
     1021 aaatgtaacg ggtgctctcg cagaaattaa tggacagcag ttattaatta gaatgaatat
     1081 tgacagtatt atgtcgggaa atttttcgta tgatacttta tttaattttg aaaacactat
     1141 cccggcaaat tttgtttttt ctgatgatgg aaagtatttg tttggtacct cttattactc
     1201 gggagtttca aatatttaca gatatgatct tgataagaag gatatggaaa taatgagtaa
     1261 tagtgaaaca ggtttcttca gaccagttcc aatttcaatg gattctattc ttgctttcaa
     1321 gttcactagt gaaggttttc aaccaattat attagcaaat aaacctgagt taaatgttaa
     1381 tgctattgat tttctgggac agaagattgt tgaacagcat cctatagtag aagactggat
     1441 agcgcctccg ccctcatcaa taaacattga ttcagtaatg acagatccta aagcttatag
     1501 tgtttttaca gattttggta ttgtttcttt gtatcccgtt gttcagggct ataaagatta
     1561 tttttcatat ggtctgcgtt ttaatctttc agatcctata ggatttcaaa atattcattt
     1621 gactgcttcc tacagtcctt attctcagct ttcggtaaac gaaaggtatc atcttgattt
     1681 tgcttattct tatcttaatt ggaaatttga atcaacttac aacagagccg atttttatga
     1741 tctttttggt ccgacaaaat cgagccgtaa aggatactct tttggattaa attatcataa
     1801 aaatgttatt tatgattcac ccagaattat ggattataca atattcacaa attattatgg
     1861 aaatttggaa cgacttcctg actatcagaa tgttagtgtt aattatgaca actattttaa
     1921 tgcgggaatt gattttaatt atcaggatct cagagcttcc cttggttcag tagatcgtga
     1981 aaaaggttat agattaaaat taagttcaaa taataatttt gttaataaaa caatttatcc
     2041 tcatataaac acaaattttg attttggttt tgcgttgccg ataaatcatt cttcggtatg
     2101 gttaaggagt tctgtcggat attcctatgg taatagactt gaaccattgg ctaattttta
     2161 ttttggcggt ttcggaaata actatgtgga taatttagat gaaaaaagat atcgcgaata
     2221 ttacagtttt cccggagttg aactcaatca tataggcgga acaaactttg ggaaattaat
     2281 gtttgaatgg aacttacctc caattcgttt cagcagcata gggttctcat ctttttttct
     2341 gaattatgcc cgcacttcat tgttttctac agtaataaca acaaacatcg attcagataa
     2401 ttaccgcaga tcattagcaa atattggtgc acaagtagat tttaaattca ttatgttttt
     2461 ccatcttaaa atgacatttt cttttggcta tgcaatggca tttgaaaaag atcaatccag
     2521 atcagatgaa ctgatgtttt cattaaaaat tttatagttt aatgatcaaa atacttatta
     2581 gtcttctgcc ggtttttatc tttctgctta tattaattta tctggacagc tataaacttg
     2641 taaagattac taatgtgatt ttattaattt ttatgggatt tgttgctgca attttaagtt
     2701 atgttatcaa tgactttctt ctcgcaaatc tgtcagtcga tattaccact tatgcaaaat
     2761 atgcttctcc ggtaatagaa gagatattaa aagcttcttt tattatttat acgatccgta
     2821 aaaagaagat agggtttatg gtagatgctg ctatctatgg ttttgcaatt ggtgctggat
     2881 tctctttcat agagaacata tactatttaa acgttattga atcatcgaat atatttctat
     2941 ggattataag aggatttgga actgctgtta tgcatggtgg tactacggct atattttcaa
     3001 tattaacaaa aaatttcttt gataggtcga ataaaagcaa atttaaatat tatttaccag
     3061 gtttggttgc tgcaatattg attcactcat tttttaatca cttactattt ccagcaatta
     3121 caattacaat ccttcaactt atttttcttc caatattaat aatttttaca tttcataaaa
     3181 gtgaacaagc attaaaagat tggatggaaa tcggtctgaa taacgatgtg caaatactgg
     3241 aacagattga taatgggatt ttttcggagt cgcatgcagg gcagtatctt ttatcacttc
     3301 aaagcagata ttcaggtaca attttagcag atatgctttg tctgattaaa attcattctg
     3361 aattgtcaat aagagccaaa ggagttatac taatgagaaa agtaggtctt ccggttatta
     3421 ttgaggatga agtaaaagag aaattagaag aattaagata cctggaaaaa agtataggcc
     3481 cgactggtag attagctatt gcaccaattt ttaatataag tacaaaagat ctttggcagt
     3541 tatatatgct tggattaaaa taagttatta ccacagaaat attaaattag tatttagcaa
     3601 tagagttacc cgtccagtaa ttgaagaaag tattgatagt ggtgaaatct actttaaatt
     3661 tatttcaact ctaaccaagt ctaaaaactt ttgttaccca ggctctgctt acatttaatt
     3721 ttcttgcaag agcagctcgg tttaaaactg ttccattatt aattaacctt ttatattcac
     3781 tcgccaaatt tttgtagtct attttaactc tcggactatt tattattttg gtttcaggta
     3841 aatagaatcc ctctgttaac tgataacatt tcttgagact tagattcaaa taattaggta
     3901 attcttctct gtcaatagtc aatttgagtt taccatttgt tgattttgca ataaaaatag
     3961 gtaattcaag cctattatgt tttgagtgcg tatcgtgttt acggagccaa tgaccccgaa
     4021 gttgcgaact tagcaattcg gggttaattg ttttaggaca gttcgacttt gattttccat
     4081 ctgctctaat cgaaatttga agttccccag atttccttcc tctttcttta taaaaaatga
     4141 tctcaacaaa tgaagcttgt taagaagtct tttggattca atcgaaagcg atctctatat
     4201 tttattgaaa tccttaaaga ttcctctaag gatcgattga ttaatattct ggcactttaa
     4261 taagacaatt tctttattca gcatattaat ttgttcgatc aactgtggtt atttgcagtt
     4321 ggatttttta taatgctggc gcaataattt atgcaataaa aaaacctggt ctatctcccg
     4381 gttttacaaa atatttcatg tatttatttt attaggaaca ttttctcatt tctggcttat
     4441 gtataattat ataagcttat ttaggtactg aggtatgcat taaacgatta aaaaaaatta
     4501 aattatattg aagaggttat gtcagattta gaata
//
LOCUS       DASWBY010000434         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_505953, whole genome shotgun sequence.
ACCESSION   DASWBY010000434 DASWBY010000000
VERSION     DASWBY010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505953"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1932
                     /locus_tag="VF870_07785"
     CDS             <1..>1932
                     /locus_tag="VF870_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252128.1"
                     /translation="NEALLYITDPNQDPFLRNPFQDTTTFKDFYGYSWRPHWDADENA
                     NWRSYSDLNNNGVWDSNEPLNNDVGSDGLGLFDTGYPGPDFDGTEANGKPDQGEPNFG
                     ILDKDESDQLGLTGFKIYAVHTYELDNDEENWGVLSGLPAPHGQTLIGVNLANYFSCY
                     LFSMNGRNTYSVTTGEIQEEGETERFSMSMIFGISKDDLFRRKKTVQQIYNANYRFAK
                     PPEKPILTAIPGDKKVTLYWDDRAEKSFDEFYQRVNFEGYRIYRSTEPNFLENKIITD
                     AYGKSTFRLPLVQYDLIDDIKGLHPIDVNGAMFYLGDDSGLKHSFIDTTVQNGQTYYY
                     AIAAYDQGFVTTNITGVMEGIPPSETTTTLKKDINGNITTDINTAVITPRTTAAGYLA
                     PEVASFEGTGPGTGNISVTILNPDSIRHLNTYRIIFDDDLPYHNSAHPDFSLINVLAS
                     DSLIKQKTITDVEEQTPVIDGFTIDIKSDTAVMVDFEKTGWKTGSSNYIVQVGFDSRY
                     QSAYIGKKIDYPADFEITLTEPGQGDLSYPATAFSQPIQSNITIKNLTENTEHFQFIF
                     RDNNSDGIYNENDAIFLAFGDSLGKRATNNSNLHVSWSISLFKDTTIAESDQRPPEFG
                     DVFKVVNKKPFRKDEFYEFT"
BASE COUNT          641 a          332 c          371 g          588 t
ORIGIN      
        1 aatgaagctt tactatatat aacagatcca aatcaggatc catttttaag aaacccgttt
       61 caagatacaa ctacatttaa agatttctac ggatattcct ggagacctca ctgggatgca
      121 gatgagaatg cgaactggag atcatattct gatttaaata ataacggagt ctgggattca
      181 aatgaaccgc taaataatga tgtgggaagt gatggtttgg gattatttga taccggttat
      241 ccgggaccgg attttgatgg aactgaagca aatggtaaac cggatcaagg agaacccaac
      301 ttcggaatat tggacaaaga tgaatcagat cagcttggtt taaccgggtt taaaatttat
      361 gctgttcata cttatgaact tgataatgat gaagaaaatt ggggagttct ttccgggcta
      421 cctgcacctc acggccagac acttattgga gtaaatctgg caaactattt ctcctgttac
      481 cttttcagta tgaatggaag gaatacttat tcagttacaa caggtgaaat tcaggaagaa
      541 ggtgagacgg aaagattttc aatgtcaatg attttcggaa tcagcaagga tgatctgttt
      601 agaagaaaga aaactgttca gcagatttac aatgctaatt atcgatttgc caaacctccg
      661 gaaaaaccaa tcttaaccgc tatccccggt gataaaaaag ttactcttta ttgggacgac
      721 agagctgaaa aatctttcga tgaattttat cagcgtgtaa attttgaagg ttaccggatt
      781 tacagatcaa ctgaaccaaa ttttttagag aataaaatta tcacagatgc ttatggtaaa
      841 tcaactttca gattacctct tgtacagtat gatttaattg atgatataaa aggccttcat
      901 cctatcgatg ttaatggtgc gatgttttat cttggcgatg attctggtct aaaacattct
      961 tttattgaca cgactgttca gaacgggcaa acctactact atgcaattgc tgcatatgat
     1021 cagggttttg ttaccacaaa tattaccggc gttatggaag gaattccgcc gtcagaaaca
     1081 acaactactt taaaaaaaga tataaatggt aatattacaa ctgatattaa tactgctgtt
     1141 ataacaccaa gaacgactgc cgccggttat cttgcacccg aagttgcttc atttgaagga
     1201 acaggacccg gaacgggaaa tatttcagtg acgattttaa atcctgattc tataaggcac
     1261 ctaaacactt atcgaattat ttttgatgat gatctgccat atcataattc agctcatcct
     1321 gatttctcat taataaatgt tttggcctct gattctttaa tcaaacagaa aacaattact
     1381 gatgtggaag aacagactcc tgttatagat ggatttacaa ttgatataaa aagtgatact
     1441 gccgtgatgg tagactttga gaaaaccggc tggaaaacag gaagcagtaa ttacatcgtt
     1501 caggttggat ttgattccag gtatcagtct gcttatatcg gaaagaaaat tgattatcct
     1561 gcagattttg aaattacttt gacagaaccc gggcagggag acttatctta tccggcaact
     1621 gcattcagtc aaccaattca atctaatatt actattaaaa atttgaccga aaacaccgag
     1681 cattttcagt tcatttttcg tgataataat tccgatggaa tatataatga aaatgatgca
     1741 atcttcttgg ccttcggtga ctcacttgga aaaagggcga ccaataattc caatttgcat
     1801 gtaagttggt ctatttctct ttttaaagac acaactattg ctgaatctga tcagagacca
     1861 cccgaatttg gtgatgtatt taaagtcgta aataaaaaac catttagaaa agatgagttt
     1921 tatgaattca ct
//
LOCUS       DASWBY010000435         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_505980, whole genome shotgun sequence.
ACCESSION   DASWBY010000435 DASWBY010000000
VERSION     DASWBY010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505980"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1714)
                     /locus_tag="VF870_07790"
     CDS             complement(<1..>1714)
                     /locus_tag="VF870_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9252129.1"
                     /translation="QTFLSLTDTGVEEFIKLHPEYDGRGTIILVLDTGVDMGIDGLTK
                     TSTGEVKVIDVQDFTGEGDLPLVKADLTTKDGKDVFENDDKGYSVFSDKNKMLKSADD
                     NYWMTVLNETHLMNSGSGAQDLNGNGVKDDKYFMVTYKTSEGYWVVYFDTNGNGDLSD
                     EKPFRNYKENFDSFTIQNQKGLTPLTFALNIFPEEKRISLFFDDGGHGTHCAGIAAGF
                     NIGNVGINGVAPGAKVIGLKLGNNNYPGGATVTESMKKAYLYADKISKEMKVPCIVSM
                     SFGIGSEIEGRSEIEKFLADLLSKNPYLYVSTSNGNEGPGISSAGLPSSSSYAFSSGA
                     VLTQEIARDNYGNILPYNILLHFSSRGGEVSKPDVISPGAATSTVPNFDGGDKKWGTS
                     MACPYTSGVMSLLLSAAEKEFPGVKIPSQLLFKIIREGAVKLDDYTALDQGAGYINVL
                     NAYDILKKYLKNNEILKFETYTVSSFAPNQPDNRSRNLYIRDGSFLTGDEVFSFNVKR
                     DNSIKSNKFYRVYNLKCDADWLTLVQKKTYIRNDQVAAVNVKLDNSKIKEPGLYTTKI
                     SAYRD"
BASE COUNT          526 a          351 c          260 g          577 t
ORIGIN      
        1 atcacgataa gcggaaattt ttgtcgtgta gagtccgggt tctttaattt ttgaattatc
       61 tagtttaaca tttacagcag ccacctgatc atttcttatg taagtttttt tctgtaccag
      121 agttaaccag tcagcatcgc atttcaaatt atatacgcga taaaatttat tggacttaat
      181 tgaattatcc cttttaacat taaatgaaaa aacttcatct ccggttaaaa acgatccatc
      241 acggatataa agatttcttg atctgttatc aggttgattt ggtgcgaatg aagaaacggt
      301 gtaagtctca aattttaaaa tttcattatt ctttaaatac tttttaagaa tatcataagc
      361 atttaaaaca tttatatatc ccgcaccctg atctaatgct gtgtaatcat ctaatttaac
      421 cgcaccttcc ctaattattt tgaatagaag ctgtgatgga attttcactc ccggaaattc
      481 cttttcagct gcacttaata ataaagacat gaccccggat gtatatgggc atgccatact
      541 tgttccccat tttttatcac caccatcaaa attaggtacg gttgaagttg cagcaccggg
      601 agaaattaca tcgggtttgc ttacttctcc gccgcgggaa ctaaaatgca gaagaatatt
      661 gtaaggaaga atattaccgt aattatcacg agcaatctcc tgagttaaca cagctcctga
      721 tgaaaaagca tagctgcttg atgaaggtaa acctgcagat gagatcccag gtccttcatt
      781 accattgctt gtacttacat acaagtatgg atttttgctt aataaatctg caagaaattt
      841 ttctatttca gaacgtcctt caatttcact tccaatgcca aaactcatgc taacgatgca
      901 tggaactttc atctccttgg agatcttatc agcatataaa taagctttct tcatactttc
      961 tgttacagtc gcaccaccgg gataattatt gttaccgagt ttcagtccga taactttagc
     1021 tccgggtgct acaccgttaa taccaacatt tccaatatta aatccggcag caattcctgc
     1081 acaatgtgtt ccgtgcccgc catcatcaaa gaaaagagaa atcctttttt cttctggaaa
     1141 tatatttaaa gcaaaagtaa ggggagttaa tcctttttga ttttgaattg tgaatgaatc
     1201 aaaattttct ttgtaattac ggaatggttt ttcatcagaa agatcgccgt tgccgtttgt
     1261 atcaaagtaa acaacccagt aaccttcgct agttttgtaa gtaaccataa aatatttatc
     1321 atcttttaca ccgttgccat ttaaatcctg tgctcctgaa ccggaattca tcaaatgtgt
     1381 ttcattcaaa acagtcatcc aataattatc atcagcactt tttaacattt tatttttatc
     1441 tgaaaaaacc gaatatccct tgtcatcgtt ttcaaaaaca tcttttccat ctttagttgt
     1501 taaatcagct ttcaccaatg gcaaatcacc ttcaccagta aaatcctgta catcaataac
     1561 ctttacttct ccggtagaag tttttgtaag tccatctatt cccatatcaa cgccggtatc
     1621 aagcactaaa ataattgtgc ctcttccgtc atattccggg tgaagcttaa taaactcttc
     1681 aactcccgta tctgtaagtg agagaaatgt ttgt
//
LOCUS       DASWBY010000436         6728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_243839, whole genome shotgun sequence.
ACCESSION   DASWBY010000436 DASWBY010000000
VERSION     DASWBY010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243839"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VF870_07795"
     CDS             <1..838
                     /locus_tag="VF870_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252130.1"
                     /translation="ARSGNSPESSQVILLSEVLSKKVHHLIITCNPESELTRTVSNKH
                     HFTFLMPPEADDKGLAMTGSFTTMLLAGLLITRINKFEGLEEQFNILYKYGNKILDEY
                     LDAIKKAAALNFKRAVFLGSGMFKGIARESQLKLQELTDGKIICKYDSFMGFRHGPKA
                     VIDEDTLVVYLFSNNEHVNLYEIDLVNAIVSGRRNLFSIGVMEQEIKIPGIDLQIILS
                     ENGKKIHEDFLTIVSVLPAQLLGLYKSLNLGLNPDSPSSSGMIHRVVQGVKVYPYSKI
                     KE"
     gene            835..2154
                     /locus_tag="VF870_07800"
     CDS             835..2154
                     /locus_tag="VF870_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HEX9252131.1"
                     /translation="MIAVENKILQLVSRHKSGKAVGITSVCSANLYVIKAAIFNAKKH
                     NQVLLIESTSNQVDQFGGYTGVTPIQFRKLVFDLADNLDFPGEHIILGGDHLGPNRWQ
                     NENSDSAMQKAKDQIAAYISAGYTKIHLDASMKCADDGSEKTPLDPSVIAERSAILCK
                     VSEETFIKSGNDTELPVYVIGTDVPPPGGSTEHHNEINITSAKEVEQTIELTKKAFEK
                     YNLQDAWNRVVAVVVQPGVEFGDEVVFDYDRNKSRELVKVIKNYPNIVYEAHSTDYQK
                     KELLRQMVEDHFAFLKVGPWLTFAFREAVFALALIEKELLSGKRGIILSNLLEVVDER
                     MINFPNYWEKYYDFSEQENKLKRKYSYSDRIRYYWADKIVNESLQRLIHNLTENKIPH
                     ILLSQYLSEEYFAVREGRISENPEEIILHKISKVTDIYNFATNGGVK"
     gene            2151..3068
                     /locus_tag="VF870_07805"
     CDS             2151..3068
                     /locus_tag="VF870_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9252132.1"
                     /translation="MKVRPWLIYAIVTTTFWGVWGALIELPEKAGFPATLGYIVWSLT
                     MIPPALVALKIINWKLEKDKKSILLGCIIGFTGAGGQLVLFQALRTGPAYLVFPFISL
                     SPVITIILSYIFLKERASRKGWIGIVLALVAIIFLSYQPPNDANSSGFLWVILSLIVF
                     AAWGLQAYIMRFANQTMKAESIFFYMMITGLLLIPFALLMTDFSQEINWGFKGPYLAA
                     IIQILNAIGALTIVYAFRYGKAIIVSPMTNAVAPVITIVLSLIIYQVLPHPVIMVGMV
                     LALVSIFLMAIEDSPEETHTEILTNKINE"
     gene            3061..4011
                     /locus_tag="VF870_07810"
     CDS             3061..4011
                     /locus_tag="VF870_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX9252133.1"
                     /translation="MSNKKFDVIVVGELNVDLILNSIDSFPEMGKEKIAKDMTITLGS
                     SSAIFASNLSSLGAKVAFIGKIGDDAFGNLVKESLSAKKVNTDYLITSDKWNTGATIV
                     LNYAEDRAMVTYPGAMEHLTMNDISEEAISAAKHLHFSSYFLQPGMKEGITALFKMAK
                     EKGLTTSLDIQWDPNEKWDFNYKEVLPFVDIFLPNEQEALLLTGESELDSAIKRLAPY
                     AKNIVVKLGSKGSVVSANGKSIFKKSFLNENVVDAIGAGDSFNAGFIFRFINGYDIDE
                     CQTFGNLTGAVNTTAAGGTAAFNNYEQIVKTGKEKFGYKN"
     gene            4008..4832
                     /locus_tag="VF870_07815"
     CDS             4008..4832
                     /locus_tag="VF870_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX9252134.1"
                     /translation="MKLIEKLKEFEYQKKALLAANFYNLETCRGIVRAAGTLECPVIL
                     QLTPGSIKYMGLKTAAGIARTVSAEENVRTWLHLDHCNDILLIKACLDEGFDSVMIDA
                     SDKNIDDNIILTKQVVKIAEAYNVNVEAELGYVPKPNSEIENDKFTEPADAKRFAEET
                     GITSLAIAIGSKHGFYKGEPKLDIDRLIAIKNVTDVYLVLHGASGIPSETLREAIKNG
                     ITKVNVATDTKDLFMKTLKEIMKNSDDIDLRNVFPPAVIEIQKMIEQKIKTVSMIN"
     gene            4848..>6728
                     /locus_tag="VF870_07820"
     CDS             4848..>6728
                     /locus_tag="VF870_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252135.1"
                     /translation="MKKIYTITLILFTCLLFNLSAQQNWTHKVRIAGNPLSNDEIDTI
                     IKNAKDSFVFGIEVDNDITGRYESFLDPTEKLEAIRKVTEEAHKINNKTFVYIAGLEC
                     ITSDADNKKNTFYKDHPDWVQRDINGRPAIFGSNDAFWISEGDEDVWISPYATEWRKI
                     YMQRVREIAATGIDGIYVDIPYWMTHFDGWENTWASFDDYTVAEFKKQTGLDAKTDIE
                     LANFKDPGFIKWINFRMQTLTDFMAEIDKNVKEINPECKTIAEIYPGISEEAVRVGAD
                     VFKMYDVVDVIAHEYSEGEYYASDRAAYDWYNYIIGMHTFRAFAEEKASWMLSYSWLD
                     NKKVMPSDAMKSLFVSQMFSGTNMWDVKGYIMSETNDMAARTEVYKWVSEYEDIFYSQ
                     RTPLEPISVYFSDITRNYFSEEFINSYRGIVSLLMHSHLPFQIVTSRTLEKLSPKILI
                     LPDVKCASIKEINSLKTLKENGTKLIATGEFASYDENLNKQNSSLPGDLIILKDCPGK
                     AYTENLKDELNEYFNSGELDTEEMLGTKNIFLSKLFELTSYNSKFKIDAPVELITTTS
                     IDEKYIYLFITNIKGICEACDTENKNITDIKISYTSSIGGDEVYTLPFLGKKEKVKTL
                     KTE"
BASE COUNT         2333 a         1029 c         1381 g         1985 t
ORIGIN      
        1 tgccagatcg ggcaatagcc ctgaaagttc acaagtaatc ctgttgtctg aagtattatc
       61 aaaaaaggta catcatctaa taattacctg caatcctgaa agtgaattga cccggacagt
      121 ttcaaataaa catcatttta cttttttgat gcctcctgaa gcagatgata aggggcttgc
      181 catgaccggc agctttacaa cgatgctgct tgccggtttg ctgataacaa gaattaataa
      241 gtttgaaggt ttagaagaac agtttaatat actttataaa tacggaaata agattcttga
      301 tgaatattta gatgcgatta aaaaagcagc tgccttgaat tttaagcgtg ctgtatttct
      361 cggctccgga atgtttaagg gaattgccag agaatcccag ctgaaattgc aagaactgac
      421 ggacggaaag ataatttgta aatatgattc ttttatggga ttccgccacg gaccgaaagc
      481 tgttattgat gaagatacgt tagtcgttta cctgttttca aacaatgaac atgttaatct
      541 ttacgagata gatttagtca atgcaatcgt ttccggcaga agaaatcttt tctcgatcgg
      601 tgtaatggaa caggaaataa aaatacccgg cattgatttg cagatcatcc tttctgaaaa
      661 cggaaagaaa attcatgaag attttttgac aatagtctca gttctgcccg ctcagttatt
      721 gggattatat aaatcattaa acctcggatt aaatccggat tccccttcaa gcagcgggat
      781 gattcataga gtggtacaag gagtaaaagt ttacccatac agcaaaatca aagaatgatt
      841 gcagtagaaa ataaaatatt acagttagta agcagacaca aatcaggtaa agccgttggt
      901 attacatctg tttgttcagc aaatctttat gttataaaag ctgcgatatt taatgctaaa
      961 aaacataatc aggttttact aatcgaatct acatcgaatc aagttgatca atttggcggt
     1021 tatacaggcg ttaccccaat ccaatttaga aaactagttt ttgatcttgc agacaattta
     1081 gattttcccg gtgagcacat tattcttggc ggtgatcatc tgggaccgaa cagatggcag
     1141 aatgaaaatt ctgattcagc gatgcagaaa gctaaagatc agattgccgc atacatatca
     1201 gccgggtata caaagatcca tctggatgca agcatgaaat gtgctgatga tggcagcgaa
     1261 aaaacaccat tagatccttc tgttattgcg gaaagatctg ccatcctctg caaagtctct
     1321 gaggaaactt tcatcaagtc cggcaatgat actgaacttc cggtttatgt aatcggtacg
     1381 gatgttccgc ctccgggagg ttcaacagaa catcacaatg aaattaatat tacttcagct
     1441 aaagaagtag aacagacaat tgagcttacc aaaaaagcat ttgaaaaata caatctacag
     1501 gatgcgtgga atagagtagt agccgttgta gttcagcctg gagtcgagtt cggtgatgaa
     1561 gtagtttttg attatgacag aaacaaatcc cgggaactgg ttaaagtaat taaaaattat
     1621 ccgaacattg tatatgaagc ccattcaaca gattatcaga aaaaggaatt attaagacaa
     1681 atggttgagg atcactttgc atttctgaaa gttggtccat ggcttacatt tgcttttcgt
     1741 gaagcagttt ttgcattagc attaattgaa aaagaattgc tgtcgggtaa gagaggaatt
     1801 atactttcaa acttgttaga agtcgttgat gagagaatga ttaatttccc aaactactgg
     1861 gagaagtatt atgacttttc tgaacaggaa aataaactta aaagaaagta cagttacagc
     1921 gatagaataa gatattattg ggcggataaa atagtaaatg aatccttgca aagattaatt
     1981 cataatctga ctgaaaataa gattccgcat attttattaa gccagtattt atccgaagag
     2041 tactttgcag tcagagaagg caggatttca gagaatcctg aagaaattat cctgcacaaa
     2101 atttctaaag tcacagatat atataatttc gcaactaacg gaggtgtcaa gtgaaagtcc
     2161 gaccctggtt aatatatgct attgtcacta caaccttctg gggagtatgg ggagctttga
     2221 tagaacttcc tgaaaaggca ggatttccgg cgacacttgg ctacatagtc tggtcgctta
     2281 ctatgattcc cccggcttta gttgcattaa aaattattaa ctggaaatta gagaaagaca
     2341 aaaaatcaat attgctcggg tgtataatcg ggtttacagg tgcaggagga cagcttgtac
     2401 tgttccaggc attaagaacg ggtccggcgt atctggtttt tccattcata tcgttgtcac
     2461 ccgtaataac aattatttta tcctatattt tcttgaagga aagagcatcg agaaaaggct
     2521 ggattggaat tgtgctggct ttagttgcaa tcatcttttt atcataccag ccgccgaatg
     2581 atgcaaacag ttccggtttc ctgtgggtaa tactttcatt gatcgtattt gctgcctggg
     2641 gactgcaggc ttatataatg agatttgcta accagaccat gaaagcagaa agtattttct
     2701 tttatatgat gataacagga cttttattaa taccttttgc acttttaatg acagattttt
     2761 cgcaggaaat aaattgggga tttaaaggac cttacctggc tgcaattata cagatactta
     2821 atgcaatagg tgctctgaca attgtttatg cattcagata cggtaaagca ataatagtca
     2881 gtcctatgac aaatgcggtc gcacctgtaa ttacaattgt gctttcactt attatttatc
     2941 aggttctacc tcatccggtg attatggtag gaatggtttt ggctttagtt tcaatatttc
     3001 tgatggcaat agaagacagt cctgaagaaa ctcatactga aatattaaca aataaaataa
     3061 atgagtaata aaaaatttga tgtaatagta gtcggcgaat taaatgttga tttaattctt
     3121 aattcaattg attcattccc ggaaatgggc aaggaaaaaa ttgcaaaaga tatgaccatc
     3181 actttaggta gttcatcagc gatttttgcg agcaacttaa gttctttggg tgcaaaggtt
     3241 gcttttatag gaaagatcgg cgatgatgct tttggaaatc ttgtaaaaga aagtttatca
     3301 gcaaaaaaag taaatactga ttatctgatt acaagtgata aatggaatac aggtgccacg
     3361 atagtgctta attatgcaga agacagagcg atggttactt atccgggtgc aatggaacat
     3421 ttaactatga atgatatatc agaagaagca atctctgcgg caaaacattt gcatttttca
     3481 tcatatttcc tgcagcctgg aatgaaggaa ggaataactg cactgtttaa gatggcaaaa
     3541 gaaaaaggat tgacaacctc gcttgatatt caatgggacc ccaatgagaa atgggatttt
     3601 aattataagg aggtacttcc gtttgttgat atttttctgc ctaatgagca ggaagctttg
     3661 ctgctgacag gtgaaagcga attggatagt gcaataaaga ggctggctcc gtatgcaaag
     3721 aatattgtag ttaaacttgg aagcaaagga tctgttgttt cagctaatgg taaaagcatt
     3781 tttaagaaat cattcttaaa tgaaaatgtt gttgatgcaa ttggtgcggg agacagcttt
     3841 aacgcggggt ttattttcag gttcattaat ggttatgata ttgatgaatg ccagacattc
     3901 ggcaacttaa cgggcgctgt caatacaacg gcagcgggcg gaacggcagc attcaataac
     3961 tatgaacaaa tagttaaaac cggtaaagaa aaattcggat acaaaaattg aaactgatag
     4021 aaaaattaaa agaatttgaa tatcaaaaaa aagctttact ggcggctaac ttttataacc
     4081 tggaaacctg cagaggtata gtaagagcgg caggaacgct ggaatgtcct gtgatacttc
     4141 agctaacgcc aggttccata aaatatatgg gactgaaaac agcagccggt attgcaagaa
     4201 ctgtttcagc tgaagaaaat gtgcgaacct ggcttcatct ggatcattgc aatgatattt
     4261 tattgattaa agcatgtctt gatgaaggat ttgattctgt tatgattgat gcaagtgata
     4321 agaatattga tgataacatt atactgacga aacaggttgt taaaattgct gaagcgtaca
     4381 atgtaaatgt cgaagctgag ttgggttatg tccctaaacc aaacagtgaa atagaaaatg
     4441 ataaatttac agaacctgct gatgcaaaaa gatttgcaga agaaaccggg ataacttcat
     4501 tagcaattgc gatcggttcc aagcatggtt tttataaagg tgaaccaaaa cttgatatcg
     4561 ataggttgat tgcaataaaa aacgttacag atgtctattt agttcttcat ggcgcttcag
     4621 gtataccatc tgaaactctt agggaagcaa ttaaaaatgg aatcaccaag gttaatgttg
     4681 ctactgatac taaagattta tttatgaaaa cattaaaaga aataatgaaa aactccgatg
     4741 atattgattt aagaaatgta ttccctccgg ctgtcataga gattcaaaaa atgatagagc
     4801 aaaaaattaa aactgtcagt atgattaatt aaaatcagta gatgaaaatg aaaaaaatat
     4861 ataccattac tttaattctt tttacttgtt tgctctttaa tttatctgcg cagcagaact
     4921 ggacgcacaa agtaaggatt gccggaaatc ctctttcaaa tgatgaaatt gatactatca
     4981 ttaagaatgc taaggacagt tttgtattcg ggattgaagt tgataatgat attacaggca
     5041 gatatgaaag ttttttagat ccaaccgaaa aattagaagc aatcaggaag gtaaccgaag
     5101 aagcgcataa aataaataat aaaacatttg tatacatagc gggactggaa tgcattacct
     5161 cggatgctga taataagaaa aatacttttt ataaagatca tccggattgg gtgcagagag
     5221 atataaacgg cagacctgca atctttggaa gtaatgatgc tttctggata tcagaaggtg
     5281 atgaagacgt ctggattagt ccatacgcaa ctgagtggcg aaaaatttat atgcagagag
     5341 tcagggaaat tgcggctacc ggaatagatg gaatttatgt cgatatccca tattggatga
     5401 cgcattttga tggttgggaa aacacctggg caagttttga tgattacaca gttgccgagt
     5461 ttaagaagca gacaggactt gatgctaaaa cagatattga attagcaaat tttaaagatc
     5521 caggatttat taagtggata aatttcagga tgcagactct gacagatttt atggcagaga
     5581 ttgataaaaa tgttaaagaa attaatcctg aatgtaaaac cattgcagaa atataccccg
     5641 gtatttccga agaagctgta agagtgggcg ccgatgtttt taagatgtat gatgtagttg
     5701 atgtaatcgc tcacgaatac agcgaaggtg aatattatgc ttcagatagg gcagcatacg
     5761 actggtataa ttatataata ggaatgcaca cctttagagc gtttgctgaa gaaaaggctt
     5821 cgtggatgct gagttactcc tggttagata ataaaaaagt catgccttct gatgcaatga
     5881 aaagtctttt tgtttcccag atgttctccg ggacaaatat gtgggatgtc aaaggatata
     5941 taatgtctga aaccaatgat atggctgcaa gaacagaagt atataaatgg gtttcagaat
     6001 atgaagatat tttttactct caaaggactc cgctggaacc gatcagtgtt tatttctcag
     6061 atattacaag aaattacttt agtgaagaat ttataaatag ttatcgagga atcgtaagtc
     6121 tgcttatgca ttctcatctg ccattccaga ttgttacgtc aaggactctt gaaaaacttt
     6181 caccaaaaat actaatcctt ccggatgtaa aatgtgcaag tattaaagaa ataaattcat
     6241 tgaaaacttt aaaagaaaat ggaacaaaac ttattgcgac cggagaattt gcaagctatg
     6301 atgaaaatct taataaacag aattccagtc ttccgggtga tttgataatc ttaaaagact
     6361 gtcctggaaa agcttacact gaaaatttga aagatgagct taatgaatat tttaattctg
     6421 gtgaacttga tacagaagag atgctgggaa caaaaaatat atttttatca aaattatttg
     6481 aattaacatc ttataattct aaatttaaaa tagatgctcc tgttgaatta attacaacta
     6541 caagtataga tgaaaaatat atctatttat ttataacgaa tataaaaggg atatgcgagg
     6601 catgtgatac tgaaaacaaa aatataacag atataaaaat tagttatacc agctctattg
     6661 gaggcgatga ggtttatacg cttccatttt taggtaaaaa agaaaaagta aaaactttaa
     6721 aaactgaa
//
LOCUS       DASWBY010000437         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_20667, whole genome shotgun sequence.
ACCESSION   DASWBY010000437 DASWBY010000000
VERSION     DASWBY010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20667"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /gene="fusA"
                     /locus_tag="VF870_07825"
     CDS             <1..1205
                     /gene="fusA"
                     /locus_tag="VF870_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560541.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9252136.1"
                     /translation="LVKPDPNAEPVLFVFKTIAEQHVGELSLFKVYSGTVKAGLDLLN
                     EANSKTERLSQLSVLNGRNRKDVPQISAGDFGAVVKLKDTHTNNTLASKNLSVIISPI
                     EFPEAVIRSAILPKAKGDEDKIASGLHTLHEEDPSFYVKFDPEISQTIISGQGELQLS
                     LAAKRLKERYKVDVDLVEPKIPYRETIKSRVDDVEYKHKKQSGGRGQYGHVHFKMEPL
                     PRGTGFEFVNAIVGGVVPGRFIPAVEKGIIETMGKGVLSGNQVVDVKTTLHFGSYHDV
                     DSDEMSFKLAAAQCFKKGFLECKPCLLEPIYEVEVIVPDEYMGDVMGDISSKRGKILG
                     MDSDGHFQIIKAHVPLSELYKYSSQLRSLTQGRGVHKRKFSHYEEVPKDVEQKVIDEY
                     NKSREEEK"
     gene            1251..1754
                     /locus_tag="VF870_07830"
     CDS             1251..1754
                     /locus_tag="VF870_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX9252137.1"
                     /translation="MEKLWSPWRSKYIESFNSDEDKSKCIFCQMLSFDPKDSKNLLVD
                     SGEHTFTVLNLYPYNNGHLMIVPKRHTNDFSGLTSDELSESFTKLQLAEKALREILNP
                     HGFNIGANIGRVAGAGIEDHIHFHIVPRWNGDSNFMPVIGDVKIISQDLAETKLKLIQ
                     AYSELLK"
     gene            1783..2304
                     /locus_tag="VF870_07835"
     CDS             1783..2304
                     /locus_tag="VF870_07835"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252138.1"
                     /translation="MKNLLILFITIITLFGCKSSIHKPADGKSTPLVLREGLLYADST
                     ANIPFTGRNKSRMMDMKIEYDVVNGIKEGDFITYYHNDKIQMIGKMKGNKNVGEWKYY
                     FQDGSLETTGFYDNDIPTGKWTWYYPNGKVVEEGNYLNGKREGEWKNYDSTGLLDIIR
                     IYKENEMIDSVKN"
     gene            2456..2529
                     /locus_tag="VF870_07840"
     tRNA            2456..2529
                     /locus_tag="VF870_07840"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2490..2492,aa:Ile,seq:cat)
     gene            2845..2933
                     /locus_tag="VF870_07845"
     tRNA            2845..2933
                     /locus_tag="VF870_07845"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2879..2881,aa:Ser,seq:gct)
BASE COUNT         1040 a          454 c          576 g          940 t
ORIGIN      
        1 tattagtaaa acctgatcca aatgcagaac ctgttttatt tgtctttaaa acaattgcag
       61 agcaacatgt aggagaactt tcattattta aagtttattc tggtaccgta aaagctggat
      121 tagatttatt aaatgaagca aacagcaaaa cagaacgatt aagtcaatta tcagtattaa
      181 atggaagaaa cagaaaagat gttcctcaga tttcggcagg tgattttggc gctgttgtaa
      241 aattaaaaga tactcacaca aacaatacac ttgcttcaaa aaatctttct gttattatta
      301 gtccgattga atttcctgaa gctgtaattc gtagtgcaat tctacccaaa gcaaaaggcg
      361 atgaagacaa aattgcttca ggtttacaca cacttcacga agaagatcca tcattctatg
      421 taaagtttga tccagaaata tcgcaaacca ttatttccgg acaaggagag ttgcaacttt
      481 cacttgctgc gaaaagattg aaggaaagat ataaagtaga tgttgatctt gttgagccga
      541 agattcctta tcgtgaaact attaaaagca gagttgatga cgttgagtac aaacataaaa
      601 aacaatccgg tggaagagga cagtacggtc acgttcattt taagatggaa cctctacctc
      661 gcggaacagg atttgagttt gttaatgcaa tagttggtgg tgttgtcccg ggtagattta
      721 ttccagctgt agaaaaagga ataatcgaaa ctatgggaaa aggagttcta tcaggtaatc
      781 aggttgtaga tgtaaaaacc acactgcatt ttggttcgta tcacgatgtt gactccgatg
      841 aaatgtcttt taaattagct gctgcgcaat gttttaaaaa agggttctta gagtgtaaac
      901 cttgtctact ggagcctatt tatgaagtag aagttatcgt acctgatgag tatatgggcg
      961 atgtaatggg tgatatatcc agcaagcgtg gtaagatctt aggaatggat tctgatggac
     1021 atttccaaat tataaaagct cacgttccgc tttcagaact ttataaatat tcatctcaat
     1081 taagaagttt aacacagggt agaggcgttc acaaacgtaa attctcgcat tacgaagaag
     1141 tacctaagga tgttgaacaa aaagttattg atgagtataa taaatcccgt gaagaggaaa
     1201 agtaattcta aggcggaaca agtttccgcc tttttaattt aaggtgcaaa atggaaaaac
     1261 tctggtcccc ctggcgttca aaatatattg aatcttttaa ctctgatgaa gataaaagta
     1321 aatgtatatt ctgccagatg ctttcattcg atccaaagga tagtaaaaat cttttagttg
     1381 atagcggtga acacactttt acagttctta atttatatcc atacaataac ggtcatttga
     1441 tgattgtacc aaaaagacat acaaatgatt tttcaggtct taccagtgat gaattatctg
     1501 agtcttttac gaaacttcag cttgctgaaa aagctttacg tgaaatttta aatccgcacg
     1561 gatttaacat tggtgcaaat attggcagag ttgctggagc aggaattgaa gaccacatcc
     1621 attttcatat cgttccaagg tggaatggcg attctaattt tatgccggtc attggtgatg
     1681 ttaaaataat ttctcaggat cttgcagaaa caaaattaaa gctaattcaa gcttattcgg
     1741 aattgttaaa ataatttttc taaaaaaata accagaggcg atttgaaaaa tttattaatt
     1801 ttatttatta caatcattac attatttgga tgtaaatcca gtatacataa acctgcagat
     1861 ggtaaatcaa cacccttagt tttgcgtgag ggacttttat atgcagattc aactgcaaat
     1921 attcctttta ccggcagaaa taaatcgcgt atgatggata tgaagataga atatgatgtt
     1981 gtaaatggta taaaagaagg tgactttata acctattacc ataatgataa aatccaaatg
     2041 attggcaaga tgaagggaaa taaaaatgtt ggcgagtgga aatactattt ccaggatggt
     2101 tcacttgaaa caactggatt ttatgacaat gatattccga caggcaaatg gacctggtat
     2161 tatcctaacg gtaaggtagt tgaagaggga aattatctta atggaaagag agaaggggaa
     2221 tggaaaaatt atgattcaac tggtttactt gatatcataa gaatttacaa agaaaatgaa
     2281 atgatagatt cagttaaaaa ttgatttaga atttgattag gctaaaagat aaaaatctat
     2341 aatgaataca aaaccttagc ttttttaatg attaaaacta aagatattcc aaaggcataa
     2401 atttaatttc ttgatttaag taagattaaa tactattttt ggacgcaaaa ataatgggct
     2461 catagctcag ttggttagag catctgactc ataatcagag ggtcggtggt tcaagtccat
     2521 ctgggcccac ttttggccgc ttttagcggc cttttttatt gcccaaatat acctttaacg
     2581 aaattaacat tccttaattc cctgagtaag tggtgtagac taatctcttt tgatatgcga
     2641 ccaaaattct gatcctctga tgaactgacg tgggtgtagg gaatgtgcaa attatagatt
     2701 aagtagctca aagcatcctt atggaacttg accttaataa aaattataac atcaaatagt
     2761 ggaaataaat ttcaaatgaa gctatagtta tatttttaat acttcgattt atatattatc
     2821 tttgtccttc aaaatttcag ctttggagag gtgggtgagt ggctgaaacc aacggtttgc
     2881 taaaccgtcg taggggttaa actctaccgc gagttcgaat ctcgccctct ccgcaaaaag
     2941 cttctcaaat gagaagcttt tttttatgaa aatattttac acatacattt tatacagtat
     3001 ttagtttaat
//
LOCUS       DASWBY010000438         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_204367, whole genome shotgun sequence.
ACCESSION   DASWBY010000438 DASWBY010000000
VERSION     DASWBY010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204367"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VF870_07850"
     CDS             complement(<1..720)
                     /locus_tag="VF870_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEX9252139.1"
                     /translation="MKSFLIKSIIIIMTVTFTGRALYISDKSKSQLKTKTEFQLRQNE
                     LDKFYSDKKLGSFVENGITYFRLFTPNAEKVSLVIFNKVDDKNGKEYEMTRDENAVWE
                     TSLSGELYGKFYGFNVKHKDQEAVLCLDPYAKAVASYNTYFTPRKAIVVKEGVYDWQK
                     DEWIQRDWRDLIVYEMHVRDMTEHQSSGASERGTYQGLIEKGKTGGIDYIKSLGVNTI
                     ELLPSQEFANIEIPFKDSLRGK"
     gene            complement(745..1074)
                     /locus_tag="VF870_07855"
     CDS             complement(745..1074)
                     /locus_tag="VF870_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252140.1"
                     /translation="MNEIILNIYLIINKGLVTEFRVVSYEMEGGDDRKIEFLKTHAKE
                     DYPKAIIFDAPSDEKGQFMSYSKFHKLEKRGMHFQLFEDIFQKFGVAENPLICVTPVV
                     DGEIYSQ"
     gene            complement(1034..1750)
                     /locus_tag="VF870_07860"
     CDS             complement(1034..1750)
                     /locus_tag="VF870_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252141.1"
                     /translation="METWNGIISLLIACIEFLLLFNLLIFIEKNRINIIAMIMITLLA
                     SYQTMEFLMCQVELQDSFYPFLAFVIIAFLPPLNLLLALTLTSNKNQTNKWFLIFIPA
                     IAFSIYYLFVIPKFVVTSCTVLYASYHYPQGDLFGAFYYLPVLISIILFARFIPKTND
                     KKKKIIAKVLLFGSIIISLPVLLGFTLMFFKNYSIISSMESIMCKFAFIYAVCLSFIC
                     LYNSPFNDERNYFKYLFNNK"
BASE COUNT          703 a          340 c          286 g          710 t
ORIGIN      
        1 ctttccgcgt aaagaatctt taaatggaat ttcaatgttt gcaaattcct gcgaaggtaa
       61 aagttcaatc gtatttacac caagactttt tatataatcg attccacccg ttttaccttt
      121 ttcaattaga ccttgatatg ttcctctttc tgaagctcct gatgattgat gttcagtcat
      181 atcacgaaca tgcatctcgt aaactatcaa atcacgccaa tctctttgta tccattcatc
      241 tttttgccaa tcataaactc cctctttaac aacaatagct ttgcgcggag taaaatatgt
      301 gttgtagctt gcaacagcct ttgcatatgg atcgagacac aagacagctt cttgatcttt
      361 gtgctttaca ttaaatccat aaaattttcc ataaagttcg ccgctaagtg atgtttccca
      421 aactgcattt tcatcgcggg tcatttcata ttctttcccg tttttatcat caactttatt
      481 aaagataacc agcgaaactt tttcagcatt tggtgtaaaa agtctaaaat atgttatccc
      541 attttcaaca aaagaaccaa gttttttatc ggaataaaat ttatcaagtt cattttgtct
      601 taactgaaac tcagttttgg tttttaactg agattttgat ttatctgaaa tgtacaaggc
      661 tcttcctgta aatgttactg tcattattat tattatagat tttattaaga aacttttcat
      721 atctaagcct gatatttata attattattg agaataaatt tcaccgtcaa caactggagt
      781 aacacaaata agtggatttt ctgcaacacc aaatttctga aaaatatcct caaacaattg
      841 aaaatgcatg ccccgctttt caagtttatg aaatttagaa tagctcataa attgtccttt
      901 ctcatcagaa ggagcatcaa aaataatggc tttgggataa tcttctttag cgtgggtttt
      961 caggaattca atttttctgt catcgcctcc ttccatttcg taagagacaa ctctgaattc
     1021 tgtaaccaga cccttattta ttattaaata aatatttaaa ataatttcgt tcatcgttaa
     1081 aaggagaatt ataaagacaa ataaaactta aacacactgc atatataaat gcaaacttgc
     1141 acattatact ttccattgaa gatatgattg aataattttt aaagaacatt aacgtaaatc
     1201 ctaataacac gggcagactt ataataatag atccaaacaa taatactttg gctattattt
     1261 ttttcttttt atcatttgtc tttgggataa atcttgcaaa cagaataatc gatattaata
     1321 ctggcagata ataaaacgcg ccaaacaaat ctccctgtgg ataatggtaa cttgcataaa
     1381 gaactgtaca gcttgttact acaaatttcg gaattacaaa taaataataa atcgagaacg
     1441 caattgccgg aatgaatatt aaaaaccatt tattagtttg atttttattg ctcgttagag
     1501 tgagagctaa aagtaaattt aatggcggaa gaaaagctat aattacaaat gcgagaaatg
     1561 gataaaatga atcttgcaat tcaacctggc acattaaaaa ttccatcgtc tggtaagatg
     1621 caagcaacgt aatcataatc attgcaatga tgtttattct gttcttctca ataaatatta
     1681 ataaattgaa aagtaagaga aattcaatgc aagctataag tagtgatatt attccattcc
     1741 aggtttccaa acacattcca aatttttgaa gccaaaaata atgatttcct atagcaataa
     1801 tgctataatt tccacccttt aattgaagta aaaatatttt ttaatggatt cgggttaagg
     1861 taagttactc cacactaatt ataaaggata tctagatgaa aaaaaggttt ttgttacttt
     1921 ttatttttgc agtttctttc tttcccggaa taataaatac agtatttgct cagaatgaac
     1981 cagttctcta tttctgtgaa aaatatgatt ctaaaaaagg tgagattggc gtaagtgat
//
LOCUS       DASWBY010000439         3789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_317692, whole genome shotgun sequence.
ACCESSION   DASWBY010000439 DASWBY010000000
VERSION     DASWBY010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317692"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VF870_07865"
     CDS             <1..394
                     /locus_tag="VF870_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252142.1"
                     /translation="TISTGTIIKPTKTLSDELMRTYLSAAGIDSVNQVLNNIYLNEQT
                     AYTLNEDEAIYFSDDLVQLKKIPDAISYLKSLSKLYPKSFKIMSAFGDVYYKDNNNGL
                     ALKYYRSAAQIDNTNQRVNNLIKKLSTK"
     gene            488..1588
                     /locus_tag="VF870_07870"
     CDS             488..1588
                     /locus_tag="VF870_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9252143.1"
                     /translation="MIGYRFTKYDPRLESNKSSFDNLLKVFLQLLTISSGDVNEAFNW
                     MNELDRKYKMTDDNYGMGDFIDDLKKNNFIEQDESGINFIPTSKSERTIRKQALEEIF
                     GKLKQSMKGNHRTFISGQGDEPTSEKRQYLFGDLMEQIDMTDSIKNAQINHGIDEFNL
                     TEDDIEVEERDYKTITSTVLMIDISHSMILYGEDRITPAKKVAMALSELITTKYPKDT
                     LDIIVFGNDAWKITIKDLPYLEVGPYHTNTVAGLDLAGDILRRRKTNNKQIFMITDGK
                     PTCLKEGARYYKNSFGLDRKILNKTLDQAAKFRRMGITITTFMIARDPYLQQFVREFT
                     QTNNGRAFYSSLSGLGDFVFEDYIRNRKKRVR"
     gene            1596..3107
                     /locus_tag="VF870_07875"
     CDS             1596..3107
                     /locus_tag="VF870_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX9252144.1"
                     /translation="MMDISKIATLGDLKKTDYKSRSIKDELRSNMISALKNNHDPFEG
                     IFGYDETVLPELHTSILSRHNILLLGLRGQAKTRIARLLVNLLDEYIPAIDMSDLNED
                     PYNPITNEAKRIIKEQGDETKIKWIHRSERYTEKLATPDVTVSDLIGDVDPIKAATLK
                     LPYSDERVIHFGLIPRSHRSIFVINELPDLQARIQVSLFNILQESDIQIRGFKIRLPL
                     DIQFIFTANPEDYTNRGSIVTPLKDRIDSQIITHYPKSISISQRITEQEADITDQQNS
                     SVVVFPLIKEIIEQIAIEARSSEYVDEKSGVSARLTISAYENIISFAERRKIINNESK
                     AYCRISDIVGVIPAITGKVELLFEGEQEGPVKVANILIGKAIKTVFEKNFPSPELLKR
                     KKEQNPYTSITSWFSKNNIIDILSNASNKDYKNILTSIPGLQKIVEQYQPDADENLSL
                     LYMEFILHGLSEYSQLSKLRLETGFQFKDMLSSMFTMNDEEENNEDTDADYFK"
     gene            complement(3159..>3789)
                     /locus_tag="VF870_07880"
     CDS             complement(3159..>3789)
                     /locus_tag="VF870_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX9252145.1"
                     /translation="AFNPLDNQLWGSVRNPIGTPKDRIIKIDLLTGDTLRVGQTGFAV
                     NTTALTFDESGQLFGIKGTASAANDLFSIDTMTAVGTIIGSIGVADLKAFGYSLASIT
                     SIEDNSIKSPSNFILDQNYPNPFNPSTQIKFSLPISSNVRITIYNLLGEVVRELLNTD
                     MNSGVHTIQWNSDDASGRKVSSGIYFYELNANGVNGNQFNQVRKMILMK"
BASE COUNT         1382 a          597 c          628 g         1182 t
ORIGIN      
        1 aacgatttca acaggtacga taataaaacc aacaaaaaca ttatctgatg aacttatgag
       61 aacttatcta tctgctgcag gtattgacag tgtaaatcag gtactaaaca atatttactt
      121 aaatgagcaa acagcttata ctcttaatga agatgaagcg atttactttt cagatgattt
      181 agttcaactt aaaaaaattc cggatgcaat ttcctatcta aaatctcttt ctaaattata
      241 tccaaaatca tttaaaataa tgtctgcttt tggcgatgtt tactataagg acaataataa
      301 cgggcttgct cttaaatatt acagatctgc tgcacaaatc gacaatacaa accaaagagt
      361 gaacaatctt ataaaaaaat tgagtactaa ataattcctt tcaggattac aattttttta
      421 ttatctactt ctacaaatta aaccgattac aactgaacca tttttatttt atttatgttt
      481 taattctatg attggataca gatttacaaa atatgatcct aggttagaaa gtaataaatc
      541 ttcatttgat aatttattga aggtatttct tcagttatta acaatatctt ctggtgatgt
      601 aaatgaagca tttaactgga tgaatgagct cgaccggaaa tataaaatga ccgatgataa
      661 ttatggtatg ggcgatttta tagacgatct aaagaagaat aattttattg aacaggatga
      721 gtctggaatt aattttattc ctacttcaaa gtctgaaaga actatccgca aacaggcact
      781 ggaagaaatt ttcggaaaat taaaacaatc catgaagggt aatcatcgga cttttatttc
      841 aggccagggg gatgaaccga cttctgaaaa aagacaatat ttatttggtg atttgatgga
      901 acagattgat atgactgatt ccattaaaaa tgcacagata aatcatggta tagatgaatt
      961 taatctgaca gaagatgata ttgaagttga agaacgtgat tacaaaacca taacatcaac
     1021 cgtgctgatg atagatatat ctcactccat gattttatac ggtgaagata gaattactcc
     1081 tgcaaaaaag gttgcgatgg cgctttcaga attgataaca acaaaatacc ctaaggacac
     1141 cctggatata attgtatttg gtaatgatgc ctggaaaatc actattaaag acttacccta
     1201 tcttgaagtt ggtccatatc atacaaatac tgttgcaggt ttagatcttg caggcgatat
     1261 attgagaagg agaaaaacca acaacaaaca aatttttatg ataactgatg gaaaacccac
     1321 ctgtcttaaa gaaggtgcac gatactataa aaacagcttt ggattggatc gcaaaatatt
     1381 aaataaaaca ttagatcagg ctgcaaaatt cagaagaatg ggaattacaa ttacaacctt
     1441 tatgattgca cgtgatccct atcttcagca atttgttaga gaatttactc aaacaaataa
     1501 tggaagggct ttttacagca gtctttcagg tttaggtgat tttgtgtttg aagattatat
     1561 acgtaacaga aaaaaaagag taaggtgaaa ttattatgat ggatatctca aaaatagcta
     1621 cacttggtga tctcaaaaaa acagattata aatccagatc tataaaagat gaattacgaa
     1681 gcaacatgat ttctgcttta aaaaataatc atgatccatt tgaaggaatt ttcggatatg
     1741 atgaaaccgt tttaccggaa cttcatactt ccatactatc aagacataat atcctgcttt
     1801 taggattgag aggacaggca aaaacaagaa tagcaagatt gcttgtaaat cttcttgatg
     1861 aatacattcc tgctattgat atgagtgatt taaatgaaga tccatataat ccaattacaa
     1921 atgaagcaaa aagaattata aaagagcaag gtgatgaaac aaaaattaag tggatacata
     1981 gatctgaaag atatactgaa aaacttgcca cacctgatgt aactgtatca gaccttattg
     2041 gtgatgttga tccgataaaa gctgcaacac ttaaactccc ctattctgat gaaagagtta
     2101 ttcattttgg attaattccc agatctcaca gatctatttt tgtaataaat gagttaccgg
     2161 atttgcaagc tcgtatccag gtttctttat ttaatatttt acaggaaagc gatattcaga
     2221 ttcgtggatt taaaatcaga ctgcctttag atattcaatt tatttttact gctaatcctg
     2281 aggactacac aaatagaggt tcaattgtaa ctccgctaaa agatagaatt gatagtcaga
     2341 tcatcactca ctatccaaaa tcaatttcta tctctcaaag aataactgaa caggaagcgg
     2401 atatcacaga tcagcagaat tcttcagtag ttgtttttcc tcttattaaa gagataattg
     2461 aacagattgc aattgaagca agatccagtg agtatgtgga tgagaagagc ggagtttctg
     2521 caagattaac aatttcagct tatgaaaaca ttataagctt tgctgaaaga agaaaaataa
     2581 ttaacaatga atccaaagct tattgcagaa ttagtgatat tgttggtgtg atacctgcca
     2641 ttacgggaaa ggttgaacta ctatttgaag gcgagcagga aggaccggtg aaggttgcaa
     2701 atattttaat tggcaaagcc attaaaactg tgtttgagaa aaattttccc agtccggaat
     2761 tactgaaaag aaaaaaagag caaaacccat atacttctat cacatcctgg ttctcaaaaa
     2821 ataatataat tgacattcta tctaacgctt cgaataaaga ttataaaaac attctgacaa
     2881 gcattccggg acttcaaaaa attgtagagc aatatcaacc ggatgcagat gaaaatttat
     2941 ctttactata tatggagttt atcctgcatg gtttatctga atattctcag ctaagtaagt
     3001 taaggttgga aaccgggttt caattcaaag atatgctaag cagtatgttc acaatgaatg
     3061 atgaagaaga aaacaatgaa gatacggatg cggattattt taaataaaaa aagccggact
     3121 tgatcaataa gtccggcttt ataatttgaa aatgaaactt atttcattag aatcattttt
     3181 ctaacctgat taaactgatt accattcaca ccattagcat taagttcata gaaataaatt
     3241 ccactgctta cttttcttcc tgaagcatca tctgaattcc attgaatagt atgcacacct
     3301 gagttcatat ctgtattcaa caattcacga actacttcac ctaaaagatt gtaaatagta
     3361 attcttacat ttgaactgat aggtaatgag aatttaatct gtgtacttgg attaaaagga
     3421 ttaggataat tttgatctaa tataaaattt gaaggagatt taatactatt gtcttctata
     3481 gatgtaatac tcgccaagct gtatccaaat gctttaagat cggctactcc tattgaacct
     3541 attattgttc ccacagccgt cattgtatca atactaaata aatcgtttgc tgctgatgct
     3601 gttcctttta ttccgaacag ctgaccggat tcatcaaagg ttaaagctgt agtattaact
     3661 gcaaatccag tctgtccaac tctaagcgtg tcccctgtca acaaatcaat tttaatgatt
     3721 ctatctttcg gagtacctat aggatttctt acagaacccc ataattgatt atccaatgga
     3781 ttaaatgct
//
LOCUS       DASWBY010000440         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_53174, whole genome shotgun sequence.
ACCESSION   DASWBY010000440 DASWBY010000000
VERSION     DASWBY010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53174"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2347
                     /gene="lon"
                     /locus_tag="VF870_07885"
     CDS             <1..2347
                     /gene="lon"
                     /locus_tag="VF870_07885"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX9252146.1"
                     /translation="VIFPYMIFPVLVGREQSINAANYALENTKYIFLSTQKKSSIEDP
                     TPADFYLEGTVAKIVQILKLPNGLLKILVDGLLQARIIQFTDRKEFFEGKIEVIIPET
                     LVDHEMNALVRQMSQLFKDYVKISRSVPNETIAAFDNIEEPDRKIFYAAANINQSIEI
                     KQNILSKYNIKEQYYEVIKVLNSEIDILKIEKEIDNKVQENIAKTQRKFIIQEQIKIL
                     QDELGDDEESSSEFGKIKDQILKAKMPKDVEAKALEEFNKLKKTPSMSPESTVIRNYL
                     DWLVDIPWYKRTKDNLSIKHVQKILDEDHFGLEKPKERIIEHIAVLNLVKHMKGQILC
                     FVGPPGVGKTSLGKSIARALGRNFIRISLGGVRDEAEIRGHRKTYIGSMPGKIIHSMK
                     KAGTINPVILLDEIDKMSMDFRGDPSSAMLEVLDPEQNNTFNDHYIEVDYDLSQVMFI
                     TTANVRYNIHLPLQDRMEIIELPGYLDYDKLEIAKRHILPKQLVAHGIQNKNVTFSDE
                     AIKKIISEYTREAGVRNLERELATVCRKVARDIVVKESANGKAIKPNKQIIDESRIED
                     YLKTPRFRSHRHSKLNKVGSVTGLAWTSVGGEILTVDVTIMNGGGKLTLTGQLGNITK
                     ESAHAGLSYLRSKAKQLGLNPDFFKGKEIHIHLPEGAIPKDGPSAGITMAMAMLSAVS
                     GMPASNNIAMTGEITLTGSILAIGGLNEKLLAAKRNDIKTILIPKDNEIDIKEMPEKV
                     KEGLKIIPIAKLEEAIPYVFPDMKKIKPETKPKINKTKKKK"
     gene            2347..2835
                     /locus_tag="VF870_07890"
     CDS             2347..2835
                     /locus_tag="VF870_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9252147.1"
                     /translation="MSESLIINSEATIDEQYQLLVKQVKSLLQKENNLITNLSNFTAA
                     LKQTFSKISWVGFYLFDGNKLYLGPFQGKVACTEIQIGSGVCGTSASERKSIIVDDVD
                     KFPGHIACDADSRSEIVVPIFKDEKLFGVLDLDSTDYKSFDDTDKKYLEELVNFLSKE
                     II"
     gene            2841..>3838
                     /gene="dnaX"
                     /locus_tag="VF870_07895"
     CDS             2841..>3838
                     /gene="dnaX"
                     /locus_tag="VF870_07895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX9252148.1"
                     /translation="MAYQVTARKWRPQKFEDVVGQEHITATLKNAIKTNRIAHAYIFT
                     GPRGVGKTTTARILAKALNCENPIDSEPCNNCPMCKTINDSQTMDIIEIDGASNRGID
                     EIRTLRESVKYAPTRGKYKIYIIDEVHMLTKESFNAFLKTLEEPPAHTIFVFATTDIH
                     KVPLTIISRCQRFDFRRIQLDVIKKTLSNIAKEENISIDDKTLTLISKKADGALRDAQ
                     SLFDQVISFCGEKIDSETVAKMLNLIDDDLFFTISDAILEKDFNVVFQTTEKIYQNGW
                     DFIDFTDGLLEHFRNILTVILTERTELVETAEVYRTKYANYIQTFSKGDLLRILNY"
BASE COUNT         1421 a          586 c          724 g         1107 t
ORIGIN      
        1 tgtaattttt ccgtatatga tctttccagt tctggttgga cgagaacaat caattaacgc
       61 cgcaaactat gctttagaaa atacaaagta catttttcta tctacacaaa aaaaatccag
      121 tatcgaagat ccaactccgg cagattttta ccttgaaggt actgtggcaa agatcgtaca
      181 gatactaaaa ctgccaaatg gattgcttaa aattttagtc gatggattat tacaagcacg
      241 aattattcaa tttacagatc gtaaagaatt ttttgaagga aagatcgaag ttataattcc
      301 tgaaactttg gtcgatcacg aaatgaatgc gttggtaaga caaatgtctc agttgttcaa
      361 agattatgtt aagataagtc gaagtgtccc caatgaaact attgcagctt ttgataatat
      421 agaggaaccg gatcgaaaga ttttttacgc tgcagcaaac atcaaccaat cgattgagat
      481 aaaacagaat atactatcca agtacaatat aaaagaacag tattatgagg ttatcaaagt
      541 acttaactca gaaattgata ttctgaaaat tgaaaaagaa atcgataata aagttcagga
      601 aaatattgca aagacgcagc gcaagtttat tatacaggaa caaatcaaaa ttctgcagga
      661 tgagcttggc gatgatgaag aatcttcttc agaatttggg aagattaaag atcagatatt
      721 aaaagcaaaa atgccaaaag atgttgaggc aaaagcgctt gaagaattta acaagctaaa
      781 gaaaactcct tcaatgtctc cggaatcgac agtaatcagg aactatcttg attggctagt
      841 tgatattcct tggtataaaa ggacaaaaga taatttaagt attaaacacg ttcagaaaat
      901 tttagatgaa gaccacttcg gattagaaaa acctaaagaa agaattatcg aacatattgc
      961 tgtactaaat ctagttaagc atatgaaagg acagatactt tgttttgtcg gtcctcccgg
     1021 agtcggtaaa acatctttag gaaaatctat tgcccgcgcg ctaggcagaa attttataag
     1081 aataagtctt ggcggtgttc gtgatgaggc cgagattcga ggtcatcgta aaacttatat
     1141 cgggtctatg ccgggcaaga ttattcattc catgaaaaaa gccgggacga taaatcctgt
     1201 aattctgttg gatgaaattg ataaaatgag tatggatttt cgtggtgatc catcatcggc
     1261 aatgcttgag gttttagatc cagaacaaaa taatacgttt aatgatcatt atattgaagt
     1321 tgattacgat ttaagccagg ttatgtttat taccactgca aatgtaagat ataacatcca
     1381 tcttcctttg caggatcgaa tggaaataat tgaactgccc gggtatcttg attacgataa
     1441 acttgaaata gctaaaaggc atatcctgcc aaaacagctt gttgctcatg gaatccaaaa
     1501 taaaaatgta actttttcag atgaagcaat taaaaaaata atttctgaat acactcgtga
     1561 agctggtgta agaaatttgg aaagagaatt agctacggtt tgtagaaaag tagcaagaga
     1621 tattgttgtt aaagaatcgg caaatggtaa agctattaaa ccgaataaac aaattattga
     1681 tgaaagcaga atagaagatt atttaaagac accgcgtttc agatctcata gacatagtaa
     1741 gttaaataaa gttggaagtg taactggttt ggcatggacg agtgtcggcg gagagatatt
     1801 aactgttgat gtaaccatta tgaacggcgg aggtaagctg actcttaccg gacagcttgg
     1861 aaatataacg aaagaatcag cgcacgctgg attaagttat ttacgctcca aagcaaagca
     1921 gttaggattg aatcctgatt tctttaaagg taaagaaata cacattcatc ttcctgaagg
     1981 tgcaatacct aaagatggac cttctgcagg aattactatg gcaatggcta tgctgtctgc
     2041 tgtaagcgga atgcctgcct caaataacat tgcaatgacc ggcgaaataa ctttaacagg
     2101 ttccattctt gctattggtg gattgaatga aaagctactt gctgcaaaac gaaatgatat
     2161 aaaaactatt cttatcccaa aggataatga aattgacatc aaagaaatgc cagaaaaagt
     2221 taaagaaggg ttaaaaataa ttccgattgc aaaattggaa gaggccattc cttatgtttt
     2281 tccggatatg aaaaagatta aacctgaaac taaaccaaaa attaataaga ctaagaaaaa
     2341 gaaataatgt ccgagtcatt aataataaat agtgaggcaa ctattgatga gcaatatcag
     2401 cttttagtta agcaggtaaa aagtttatta caaaaagaaa ataatcttat tacaaatctt
     2461 tctaatttta ctgcggcatt aaagcaaaca ttttctaaaa ttagctgggt aggattttat
     2521 ctttttgatg gtaataaact ttatcttgga ccatttcagg gaaaagttgc atgtactgaa
     2581 atccaaatag gcagtggtgt ttgcggaaca tctgccagcg aaagaaaatc aattattgta
     2641 gatgatgtag ataaatttcc cggacatatt gcgtgcgatg ctgattcgag atcagaaatt
     2701 gttgttccga tttttaaaga cgaaaagtta tttggtgttc tggatttgga cagtactgat
     2761 tataaatcat ttgatgacac agataaaaaa tatctggaag aattagtaaa ttttttaagt
     2821 aaagaaatta tatagagaat atggcttatc aagtaactgc gagaaaatgg cgtccgcaaa
     2881 aatttgaaga tgtggttgga caggaacata taactgcaac tttaaagaat gcaatcaaaa
     2941 ccaatcgaat tgctcacgca tatatcttta ctggtccgcg cggagttggt aaaacaacta
     3001 ctgctagaat acttgcaaaa gctttgaact gcgaaaatcc aatagactcc gaaccatgca
     3061 acaattgccc aatgtgcaaa accattaatg attctcaaac aatggatata attgaaattg
     3121 atggagcatc taatcgcggt attgacgaga taagaacact tagagaatca gtcaagtacg
     3181 caccgactcg tggtaaatat aaaatttata taatagatga agtccatatg ttaaccaagg
     3241 aatcatttaa tgcattccta aaaactttag aggaaccacc agcacataca atttttgttt
     3301 ttgctacaac ggatattcac aaagtaccac ttacaattat ttcccgctgt cagagatttg
     3361 atttcagaag aatacaacta gatgtaataa agaaaacact tagcaacatc gcaaaagaag
     3421 aaaatatttc tatcgatgat aaaacactga ctctcatttc taaaaaagca gatggcgcac
     3481 tgcgcgatgc acaaagttta tttgatcagg taatatcttt ttgcggtgag aagattgatt
     3541 ctgaaactgt tgcaaagatg ttaaacttaa ttgatgatga tttatttttt actatttctg
     3601 atgccattct tgaaaaggat tttaatgtcg tcttccaaac cacagaaaaa atttatcaaa
     3661 acggatggga ttttatagac tttactgacg gattgttaga acatttcaga aatattctta
     3721 cagtaattct aacagaaaga acggaacttg tagaaacagc ggaagtctat cgaacaaaat
     3781 atgcaaacta tattcaaact ttttctaaag gtgatttact tagaatatta aattactt
//
LOCUS       DASWBY010000441         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_543605, whole genome shotgun sequence.
ACCESSION   DASWBY010000441 DASWBY010000000
VERSION     DASWBY010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543605"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VF870_07900"
     CDS             complement(<1..278)
                     /locus_tag="VF870_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252149.1"
                     /translation="MKNLFISFLVAVFIGSAFAQNQTVDSTLWQKAEQLCDKFTLVDT
                     HIDLPDWLYDEWFDVTQPGKGEIDYPRAMKGGLDVPFMSVYTSPGLEGT"
     gene            441..1484
                     /locus_tag="VF870_07905"
     CDS             441..1484
                     /locus_tag="VF870_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HEX9252150.1"
                     /translation="MKHQNIIAASILLLLFQKACVQYHDQPQHKDQKEKLVLDLSGRW
                     KFSPGDDQRWKDVKYDDRNWEKIYTPSSWENQGFHGYDGYAWYRNSFKLTSDLKTKDL
                     YLVLGFVDDVDQTFINGNLIGVSGGFPPDYRTAYNAFRKYYIPNEYLNKDGNNIIAVR
                     VYDAELDGGIMSGKIGLYTNEDGLEPDVNLSGIWDFKTGDDSSFLKNNALDSNTDKLM
                     VPAHWDVQGYPDYDGFAWYKKTFYLPKEQDGESMILMLGKIDDIDQTFVNGILVGSTG
                     LWNFNGTPTEFDKNEEYRITRIYSVPQKLLKFGEENSIVVRVYDGFKNGGIYDGPIGL
                     LKQSNYNKYFRKK"
     gene            complement(1487..2329)
                     /locus_tag="VF870_07910"
     CDS             complement(1487..2329)
                     /locus_tag="VF870_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9252151.1"
                     /translation="MKKISNKTTAYYFILIWIVAAFLLESSDLWISINLYNPDSGWAI
                     FLQKYGEISGLLVVLIGIHIYIVTIKTSSNIKTILFTGFLLTTGSLITIYIFWAFTLG
                     VSNDTLFFTVYKNYFFFAAVLLNIVLSLIFRKQFKFSKKTILFSRISFKMFFYGYILF
                     VQPLKLLWGRIRFRDLAENYSNFTPWYWPNGINGNQSFPSGHASMGFIMIALFVFIAD
                     KSFYKRIFLKGLIISWGLAVCASRVVIGAHFTSDVLFGAFPMIISYLLLINNANKTII
                     AESR"
BASE COUNT          998 a          411 c          480 g          881 t
ORIGIN      
        1 gttccttcta accctggcga agtataaact gacataaatg gaacgtcaag tccacctttc
       61 atagcgcgcg gataatcaat ctcaccttta ccaggttgtg ttacatcaaa ccattcatca
      121 taaagccaat ccggtaaatc aatgtgagta tcaactagag taaatttgtc acaaagctgt
      181 tctgcttttt gccaaagcgt ggaatcgaca gtttgatttt gtgcgaaagc gcttccgata
      241 aaaactgcaa ctaaaaaaga aataaaaaga tttttcattt atgctcaata agaatgtgga
      301 tataattcac ttagaaaata gttaatttca actatctctc aaattgaaat catgacaatt
      361 ttttacaaat gtttacttaa ttgtgatgaa gttaaagaat gaatcaatta ggtttgtttt
      421 agaaaaaaca aaatatgtat atgaaacatc aaaatatcat agcggctagt attctattat
      481 tactttttca aaaggcatgc gttcagtatc atgatcaacc gcagcataaa gatcaaaaag
      541 aaaaattggt attagatcta tccggacgat ggaaatttag tcctggtgat gatcagcgat
      601 ggaaagatgt aaagtatgat gacaggaatt gggaaaaaat ttatactcct tcttcatggg
      661 aaaatcaggg atttcatggt tatgatggtt atgcgtggta ccgaaattct tttaaattga
      721 cttctgatct taaaacgaaa gacctttatt tggttttagg ttttgtcgat gatgttgatc
      781 aaacttttat taatggaaac ctgattggtg tttctggtgg ttttcctcct gattatcgaa
      841 cagcctacaa tgcttttaga aaatattaca ttcccaatga atacttaaat aaagatggaa
      901 ataacataat tgctgtgcgt gtttatgatg ctgaacttga tggcggtata atgagcggaa
      961 aaatcgggct atacaccaat gaggatggac ttgaacctga tgtgaatctt agcggcattt
     1021 gggacttcaa aacaggggat gattcatcct tcctaaaaaa taatgctctt gattctaata
     1081 ctgataaact tatggttccg gctcattggg atgttcaggg atatccggat tatgatggct
     1141 tcgcctggta taaaaaaact ttttaccttc caaaagagca ggatggtgaa agtatgattt
     1201 tgatgcttgg caaaattgat gacattgatc agacatttgt aaatggtata cttgtcggtt
     1261 caaccggcct ctggaatttt aatggcactc caactgagtt tgataaaaat gaagaatacc
     1321 gtataacaag aatctattca gtgccgcaaa aacttttaaa gtttggagaa gaaaattcaa
     1381 ttgtggtaag agtttacgac ggatttaaaa atggtggaat ctatgatggc ccgattggtt
     1441 tacttaagca aagtaattac aataaatatt ttagaaaaaa ataaaactat ctgctttcag
     1501 caattatggt tttgttagca ttattgatta agagcaggta agatataatc attggaaaag
     1561 ctccaaacag aacatcagaa gtaaaatgtg cgcctattac aactctgctt gcacacactg
     1621 ctaatcccca gctgatgatt aatcctttta aaaatattct tttataaaaa gatttatctg
     1681 caataaaaac aaaaagtgca atcattataa aacccattga agcatgcccg gaagggaaag
     1741 actgatttcc gtttattcca tttggccaat accagggagt aaaattagaa taattttccg
     1801 ccaaatctct aaaacgaatt ctaccccaca gtaattttaa tggctgcacg aaaagtatat
     1861 agccgtaaaa aaacatttta aaagaaattc gcgaaaacaa aattgttttt tttgagaatt
     1921 taaattgttt tctaaagatt agagatagaa cgatgtttag taaaactgcc gcaaagaaaa
     1981 aatagttctt ataaacagta aaaaataaag tatcgtttga gacgcctaat gtaaaagccc
     2041 agaaaatata tattgtaata agggatcccg tagttaaaag aaatcctgta aacaaaatag
     2101 ttttaatgtt tgaggaagtt ttaattgtaa cgatatagat gtgaataccg atcaaaacaa
     2161 ccagtaagcc tgatatttca ccatacttct gcagaaaaat tgcccagcct gaatccggat
     2221 tataaagatt aattgaaatc cagagatcac ttgattccaa aaggaatgcg gcgactatcc
     2281 aaataaggat aaaataatat gctgttgttt tatttgatat ttttttcaaa ttgtttttta
     2341 tttttttatc aatttaatac ttgaaaaatt aaactactaa ttagtttatg tttgaaattt
     2401 ccgtaactct atgacttaaa aaaataggaa ttactaaata ataaaaatct ttacctgtaa
     2461 acttatatta atattcttaa tcaataaaag ttgaacggtg gctaaccttt cctaaatcta
     2521 tttttccgtt agtcacagct aagcaaaaag atttgtaatt atattgtcta atttaaaagc
     2581 tttatcgtat atttacgatg aacgattaag gaattattta aatggctgta tcaaaaaaag
     2641 aagaatttaa aattgaggat atttggctgg cggatattgc aaaagcttta tcacatcccg
     2701 cacgaataag aatattaaaa atcctgaccg aaataaatgt ttgcatgtgt ggcgatattg
     2761 ttgagcttct
//
LOCUS       DASWBY010000442         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_317956, whole genome shotgun sequence.
ACCESSION   DASWBY010000442 DASWBY010000000
VERSION     DASWBY010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317956"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VF870_07915"
     CDS             complement(<1..299)
                     /locus_tag="VF870_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252152.1"
                     /translation="MRTILVLVLVSVLTSFTLSQTVEDKILLSGNLSTDSKIIFNNYA
                     EPVTPQFMYSVENKKSPILAGVMSLLIPGSGEIYTEEYLKAGIFLAIEAAVITTAV"
     gene            complement(315..>1520)
                     /locus_tag="VF870_07920"
     CDS             complement(315..>1520)
                     /locus_tag="VF870_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HEX9252153.1"
                     /translation="RFDNYNTGNTFWEKFKPGLFVTSSDMLDRYSIFAGAAVNARWER
                     DLFLIFDYRNKLPVLSSLGLRPELSLELYSISRKADVDVFFGADTTAGGTVNYDYVTP
                     TEVTYDLFEFDFIIRHRLFTRDQNLQLKYTNSSYTAILGSFILPDENNTLYPTTKDNY
                     FNGNNLEAKFTWHAILPTKDDEINPVGFDVNLTYNYEFSDYNEKGEYTVENGLLIPKY
                     GNFDFNRLELKTSLFLPVFDSHTFNTTLRLGSILGPTVPDFFDFYLGGLIGMKAYPFY
                     AISGNEIAWLNFTYRFPLFRNIDTRAGHLYIDKIFLSFNGDIGNAWNNDATTIESFKK
                     GLGAELRIQLSSYYLFPTSVFVSASYGFDEFKRNVNNEIVTYGKEWNLYAGILFGFEI
                     LNFNKSPRF"
BASE COUNT          513 a          275 c          246 g          486 t
ORIGIN      
        1 actgctgtgg taattactgc ggcctctatt gcaagaaaaa ttccagcctt aagatactct
       61 tcagtatata tttcaccgga acctggaatt aataatgaca ttactcctgc aagaattggc
      121 gattttttgt tttccacaga atacataaac tgaggtgtaa ctggttccgc ataattatta
      181 aaaattattt ttgaatctgt ggacaagttg ccggacaata aaatcttatc ttccactgtt
      241 tgggataatg tgaatgaggt caaaacagat acaagtacta aaacaaggat tgttctcatt
      301 aaaatatcct tttattaaaa tctgggagat ttattaaagt taaggatctc aaaaccaaaa
      361 agaattccgg catataaatt ccactccttg ccataagtaa caatctcatt atttacattt
      421 cttttaaatt catcaaatcc ataggaagca cttacaaata cgctggtcgg gaaaagataa
      481 tatgaactaa gctgaattct cagttctgcg cctaaacctt ttttgaagct ttcaattgta
      541 gttgcatcat tattccaggc attaccaata tcaccattaa aggataaaaa tattttatct
      601 atgtataaat gtcccgctct tgtatcaata ttacgaaaca aagggaatct gtaagtaaag
      661 tttaaccagg ctatctcatt cccgctgatt gcataaaatg gataagcttt catgccaatc
      721 aaaccaccaa gatagaaatc aaagaagtct ggtacagttg gtccgagaat acttcctaat
      781 cttagtgttg tattaaaagt atgcgaatca aaaaccggta agaaaaggct tgtttttaat
      841 tcaagtctgt taaaatcaaa attaccgtat ttgggtatta ataaaccatt ttcaactgtg
      901 tactcgccct tttcattata gtcactaaat tcatagttgt atgttaagtt aacatcaaat
      961 cccacaggat taatttcatc gtctttagtt ggcaaaattg catgccaggt aaacttggct
     1021 tctaaattgt ttccgttaaa ataattatct ttagttgtcg ggtagagtgt attgttttca
     1081 tcgggtaaga taaaactgcc cagtatagca gtataactgc tattcgtata ttttaattga
     1141 agattctgat ctcttgtaaa aagtctgtgc cgaattataa aatcaaactc aaaaagatca
     1201 taagtaactt cagtgggcgt aacatagtca tagtttactg taccgcctgc tgttgtatca
     1261 gctccaaaaa aaacatcaac atctgctttt ctgctaatgc tgtaaagttc gagtgataac
     1321 tccggacgta atccgaggga tgaaaggaca ggtaatttat ttcgataatc aaaaatcaag
     1381 aacaaatccc tttcccatct tgcgtttacc gctgcaccgg caaaaataga atatcgatcc
     1441 agcatatcac ttgaggtaac aaaaagtccc ggcttaaatt tttcccagaa agtgttgccg
     1501 gtattataat tatcaaatct
//
LOCUS       DASWBY010000443         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_655449, whole genome shotgun sequence.
ACCESSION   DASWBY010000443 DASWBY010000000
VERSION     DASWBY010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655449"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1051)
                     /locus_tag="VF870_07925"
     CDS             complement(11..1051)
                     /locus_tag="VF870_07925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9252154.1"
                     /translation="MIGKTIKRIEKKEVFGLSRCFQLTFIMSSFFFLSSSLVSAADLQ
                     TREQILEDRMESYTKYSNLTLPWYYLAAVDQFERNIQQVRNDIPKREGTVAIQFSDDY
                     WVGVHNPQKNDTLSASIAFFDGNGMDGNNDGFADRKNDDDVMLTMAHFLSNYSHSEKE
                     LKRALKTYYKHEQSVHQIMTIAKIYEHFETLKLDQNAFPLPVNQDYSYRSTWGDRRGW
                     GGRRIHEGTDLFTSYGVPVRATTYGLIETKGWNEYGGWRVGIRDVQNTYHYFAHLSSF
                     SKEIKEGDIIKPGTIIGYVGSTGYGKKWTSGRFPPHLHYGMYKYNGRTEWAYDPYPSL
                     KRWEQQERKASN"
     gene            complement(1168..1869)
                     /locus_tag="VF870_07930"
     CDS             complement(1168..1869)
                     /locus_tag="VF870_07930"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX9252155.1"
                     /translation="MKPIIGISSNLNDHVLSVPTDYIQAITKFGGVPIVIPNIQGDAI
                     DSIVQLLDGLLLTGGGDIDPTLFNEEPLQNLGTITPERDAFEIAIIQKMMKLNKPILG
                     ICRGLQILNIAIGGDMYQDIYSQSQNKLLQHTQLAPRNHASHFVQVIKGSMLSDIVQA
                     EKFKVNSFHHQAVRKIPNDFKACAIASDGIIEAFESNIHSFVMGLQWHPENLLSKNDE
                     ASIAIFKAFILSCEK"
     gene            complement(1916..>2411)
                     /locus_tag="VF870_07935"
     CDS             complement(1916..>2411)
                     /locus_tag="VF870_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9252156.1"
                     /translation="AKVAEAFTQLKIEGQVRHFGVSNFKRSQFKMLQSYLDFPLITNQ
                     IELSAYQLENFEDGTLDLCQEERIAPMAWSPLAGGSIFSSNDERAKNLRNTLERIAGE
                     IGAKAIDEVLYAWLLSHPANIMPIVGSGKMSRIESAIRALDLTLTRDQWFEILQVAME
                     REIA"
BASE COUNT          732 a          472 c          430 g          777 t
ORIGIN      
        1 tcaatttctt ttaattggaa gccttacgtt cttgttgttc ccatcgtttg agtgaaggat
       61 aggggtcata agcccattca gttcgtccgt tatacttata catgccatag tgaagatggg
      121 gagggaaacg gccacttgtc catttcttac cgtatccagt actacccaca tacccaataa
      181 tggttcctgg cttaataata tcgccttctt taatttcttt gctaaaacta gataagtgag
      241 caaagtaatg atacgtattc tgaacatccc gaattcctac tcgccaacca ccgtattcat
      301 tccaaccctt agtttcgatt aaaccgtatg tggttgctct aacggggaca ccataagagg
      361 taaataaatc agtaccttca tgtattctgc ggccacccca tcctcttctg tcgccccaag
      421 tacttctgta gctataatct tgattaactg gaagaggaaa tgcgttttga tctaatttta
      481 atgtttcgaa atgttcgtaa atctttgcga tggtcatgat ttggtgtaca gattgttcgt
      541 gcttataata tgtttttaaa gcccttttga gttctttttc actatgtgag taattactta
      601 agaagtgagc cattgtaagc attacatcgt catcattttt tcgatcagcg aatccgtcat
      661 tatttccatc cataccgttt ccatcaaaaa aggcgatgga tgcagagaga gtatcatttt
      721 tttggggatt atgaacacct acccaataat catctgagaa ctggattgcg acggttcctt
      781 ctcttttagg aatatcattg cggacttgtt ggatgttcct ttcaaattga tctactgctg
      841 ctaagtaata ccaaggtaaa gttaagttag aatactttgt ataggattcc atccggtctt
      901 ccaagatttg ttctcttgtt tgtaggtcgg cagctgaaac aagggaggaa ctaagaaaga
      961 aaaatgagct cataataaaa gttaattgaa aacatcgact caaaccaaat acctcctttt
     1021 tttcaatacg ttttatagtt ttcccaatca tcatgtattc atgagtagaa aatgatgtaa
     1081 agatataatt gcagaaaatt acgaaacaaa aaagcggaca tttaataatg gccgctttcc
     1141 agaatgattt tataggatta attaacgtca cttctcacaa gacaaaataa atgctttgaa
     1201 aatagcaatt gaagcttcgt cgttttttga taacaaattt tctggatgcc actgcaagcc
     1261 catgacaaac gagtggatat tactttcaaa agcttcaatg ataccatcac ttgcaatagc
     1321 acatgcttta aagtcatttg gaatttttct gactgcttga tgatgaaaac tatttacctt
     1381 aaatttttct gcttggacga tatcacttag cattgagcct ttaataacct gcacaaagtg
     1441 agatgcatgg ttccgtgggg caagttgagt atgttgtaac agtttatttt gcgactgtga
     1501 gtaaatatct tgatacatat cgccacctat agcaatattc aaaatttgca agcctctgca
     1561 tatgcccaaa attggtttat taagcttcat cattttttgg atgatggcta tttcaaaggc
     1621 atctctttca ggcgtaatag ttccaagatt ttgaagaggt tcttcattaa ataatgtggg
     1681 gtcaatgtct ccacctccag ttaaaagcaa tccatcaagt aactggacaa tggagtcaat
     1741 cgcatcccct tgtatgtttg gaatgacaat agggacacca ccaaatttcg ttatggcctg
     1801 aatatagtct gtagggactg aaagaacatg gtcatttaaa ttagaagaaa taccaataat
     1861 aggtttcaaa tggataattc ctttcgcttc agtttttgat ttaaaaggtg tgaaattacg
     1921 ctatttcacg ttccatcgca acttgcaaaa tttcaaacca ctgatctctc gttaaagtta
     1981 aatctagtgc tctgatcgcg gactcaatac gactcatttt acccgaacca acaattggca
     2041 taatatttgc aggatggctg agtaaccatg cataaagaac ttcatctatc gccttagcac
     2101 cgatttcacc agcaattctc tccagcgtat tacgcaaatt cttcgctcgt tcatcattag
     2161 aagagaagat actaccacct gccagaggcg accaagccat tggagcaatt ctttcttctt
     2221 ggcaaagatc aagagtgcca tcttcaaaat tctcaagttg ataagcagaa agctcaattt
     2281 gatttgtaat cagtggaaaa tctaaatatg attgaagcat tttaaactgt gaacgtttaa
     2341 aattcgacac gccaaaatga cgaacttgac cttcaatttt caactgagtg aatgcttctg
     2401 ctacttttgc c
//
LOCUS       DASWBY010000444         4896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_431865, whole genome shotgun sequence.
ACCESSION   DASWBY010000444 DASWBY010000000
VERSION     DASWBY010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431865"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF870_07940"
     CDS             <1..360
                     /locus_tag="VF870_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9252157.1"
                     /translation="NDANKLVGSLPLGWKQKLAFSTAIIHAPRIVFLDEPTGGVDPIT
                     RRQFWDMIYEAAHHGITVFITTHYMDEAEYCDRVSIMVDGVIKALDSPAGLKKGFNAK
                     SMDEVFVKLARDAKRGE"
     gene            539..1642
                     /locus_tag="VF870_07945"
     CDS             539..1642
                     /locus_tag="VF870_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9252158.1"
                     /translation="MKQLITFVKKEFHHILRDKRSMLVLLGLPIVQLLIFGFAITTET
                     RNCNIAILDNSKDEATQNIITRIESSTYFDIYKTLSLNDQIEKEFKTGRIKLAIVFPP
                     NFQNDLLHTNKAQIQIITDASDPNQATTLSNYVSAIILEYQQELNQNKDLPYTINTEI
                     RMLYNPQLKGEFSSVPGVMGMILLLISAMMTSISIVKEKEMGTMEVLLVSPMKPSILI
                     ASKVIPYFIVSLINVITILLISNIVFAIPIQGSFILLSLITIIYIMCALALGILISTV
                     TSSQQAAMIISLMGLILPVVMLSGYAFPIANMPLILQIISNIIPAKWYIIMVKDVMIK
                     GIGLFEIWRELLIVIGMTAFFLMISIKRFKVRL"
     gene            1642..2760
                     /locus_tag="VF870_07950"
     CDS             1642..2760
                     /locus_tag="VF870_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9252159.1"
                     /translation="MRTLFFLLRKEFQQILRNKLIIRMIFALPVVQLIILPNAASYEM
                     KNINMSIVDHDHSEYSRQLINKFTSSGYFQLTNFSETYKDALVFVEEDKADLIVEIPQ
                     GFERDLIRDNKSKVMIAANAINGQASGLSVSYANSIIQDFNNEVRVEWVQPPRINPQP
                     MIDITTINWFNPKMNYKYFMVPGVLVLLVTLVGFMLTALNIVKEKEDGTIEQLNVSPI
                     KKYQFILGKLIPFWILGNIVLTMGFGVSYIVYGIFPVGNPILIYLFATVYLIALLGFG
                     LFVSTLAETQQQAMFVAYFFMTVFILLSGLFAPIENMPGWARILAYMNPVSYFIDITR
                     MIVLKGSGFTDILPNLGIITLFAIGFNILAILNYKKTV"
     gene            2811..4103
                     /locus_tag="VF870_07955"
     CDS             2811..4103
                     /locus_tag="VF870_07955"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9252160.1"
                     /translation="MANNSTKLFEEIFSEEIISNESFRSKVLAGIVGFLIIVVLLISL
                     TFEKQFEEISQFPIAFQLTIIILSVILVRSLFVSRAAKQWTRYGVKAFILIRYINAFV
                     EISIPTVALIIYSFNLPSVFPLFTPVALLYFLIIMLSALELDFKLCVFSGAVAAVQFT
                     ILAWYLSNKPLPIETIDSFSLFPVYLGTAALLFISGHTAGLITNQIKKGLIKHYRAQS
                     ERNEIQKLFGQQISKEIVDDLVKNKYEIQSRVRFATIMFLDIRNFSIFAQNKSPEEII
                     AYQNNVFSFIIEIINENKGIVTQIMGDGLMAIFGAPIEHENDCQLAVNAALEINKELK
                     RRNNAGLIPETIIRIGINAGEVVTGNVGTSERKQYSITGQPVIIAARLEQINKELNSV
                     ILISDSVYKRILLAEEPKYHKDLVIKGIPDPITVYQLA"
     gene            4172..4606
                     /locus_tag="VF870_07960"
     CDS             4172..4606
                     /locus_tag="VF870_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9252161.1"
                     /translation="MQRIKIDLPQKFLFTTEISVRVYDVNFAGHLSNDSILSMVHEAR
                     ILFLKNWGYSEVDTCGAGIIMFDAALQYKSQGYHGDILLFQVTVDNFIKNGCDFFFKI
                     TNKATGKEIARAKTGIAFFDYQSNKLVHVPEEFRALIDSLKV"
     gene            4624..>4896
                     /locus_tag="VF870_07965"
     CDS             4624..>4896
                     /locus_tag="VF870_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX9252162.1"
                     /translation="MFDNIVFTANIVAPVFLIIAVGYFAKRTKIINEVFVDVTSKFVF
                     QISLPVFIFIKISQLDLSQAFEFNQIIYIYIGTIVTYLIIWAATIKF"
BASE COUNT         1675 a          761 c          842 g         1618 t
ORIGIN      
        1 aatgatgcga ataaattagt tggttccttg ccgcttggct ggaaacaaaa acttgctttt
       61 agcacggcaa taatccatgc accaagaatt gtttttcttg atgaaccaac aggcggggtt
      121 gatccgatta ccagaagaca gttttgggat atgatttatg aagcggctca ccatggaatc
      181 accgttttta taacaacaca ttatatggac gaagctgagt actgcgatcg tgtttcaatt
      241 atggttgacg gagttattaa agctctggat tcaccagctg ggttgaagaa aggatttaat
      301 gcaaaatcta tggatgaggt atttgtaaaa cttgcccgcg atgctaaaag gggagaatga
      361 tatcttaact catttataaa acagtaaatt gtcattacga gaaacgaagt gatgaagtaa
      421 tctaaaaaaa gaaaaaaata attgatagat aaaacaaaac tgaatccagc tcgacaaaaa
      481 atattttgag attgcttctc ccgacttcgt cgggcccgca atgacataaa agaataatat
      541 gaaacaatta ataacatttg taaaaaaaga attccatcat atcctccgcg ataaaagatc
      601 gatgcttgta cttttaggtt tacctattgt acagctttta atatttggat ttgcaatcac
      661 aactgaaacc cgtaattgta acattgcaat tttagataac tcaaaggatg aggcgacaca
      721 aaatatcatt acacgaattg aaagcagcac ttactttgat atttataaga cactttcatt
      781 aaatgatcaa attgaaaagg aatttaagac aggccgtata aaattagcaa ttgtttttcc
      841 tccaaatttt caaaacgatc tgttgcatac caacaaggcg caaattcaaa taataacaga
      901 tgcttctgac ccaaatcagg caactacttt atctaactat gtaagtgcaa ttatattaga
      961 gtatcaacag gaattaaatc aaaataaaga tttgccttac acaatcaaca cagaaataag
     1021 aatgctttat aatcctcagt taaaaggcga gttttcttct gtgcctggtg ttatggggat
     1081 gattcttctg ctaatctctg caatgatgac gtctatttca atagtaaaag aaaaagaaat
     1141 gggaacaatg gaagttcttc tcgtttcacc catgaagcct tcaatactta ttgcaagtaa
     1201 agttattccc tactttatag tctcactcat taatgtaatt acaatcttgc taatcagtaa
     1261 tatagttttt gcaataccaa tacagggaag ctttattctt ttaagtttaa taacaattat
     1321 ttacatcatg tgtgcattag cattggggat tttaatatcc acagtaacaa gttcacaaca
     1381 ggctgcgatg attatttctc taatgggttt gatactgcca gttgttatgc ttagcggata
     1441 cgccttccct attgcaaata tgcctctgat actgcagatc atttctaata ttattcctgc
     1501 aaaatggtat atcattatgg ttaaagatgt gatgataaaa gggattggac tttttgaaat
     1561 ttggcgcgaa ttattaatag tcattgggat gacagcattc tttttaatga ttagtataaa
     1621 acgatttaag gtcaggttat aatgagaact ctgttttttt tattaagaaa agaattccaa
     1681 caaatattac gtaataaact tataataaga atgatatttg cgttgccggt agttcaattg
     1741 attattcttc cgaatgcagc aagttatgag atgaaaaata taaatatgag tattgttgat
     1801 catgatcatt ctgaatattc aagacaactg attaataaat ttacatcctc aggttacttt
     1861 caattaacta atttttcaga aacatataaa gatgctttag tatttgttga agaagataaa
     1921 gccgatttaa tagtggaaat tccccaagga tttgaacgag atttgataag agataacaaa
     1981 tctaaggtta tgattgctgc taatgcaatt aacggacaag catcagggct gtcagtttct
     2041 tatgctaatt caatcatcca ggattttaac aacgaagtta gagttgagtg ggtacaacct
     2101 ccccgaataa atccgcagcc tatgattgac ataacaacaa tcaactggtt taatcccaaa
     2161 atgaattata aatactttat ggttcccggc gtgctggttt tattagtaac attagttggt
     2221 tttatgttaa ccgcactaaa tatcgttaaa gaaaaagaag atggaacgat agaacaatta
     2281 aatgtttcgc ctattaaaaa gtatcaattt attttgggta aacttattcc cttctggata
     2341 ttaggtaata ttgttctcac aatgggcttc ggagtaagtt atattgttta tggaattttt
     2401 cctgttggta atcctatttt aatttatttg tttgcaacag tatacttaat tgcgctttta
     2461 ggatttggat tatttgtttc aacacttgct gaaacacagc agcaagcaat gtttgtagca
     2521 tattttttta tgacagtttt tattctgtta agcggattgt ttgccccaat tgaaaatatg
     2581 cccggctggg cacgcatact ggcttatatg aatccggtca gttactttat tgatataaca
     2641 aggatgatag ttttaaaagg aagcggcttt actgatattt tacccaatct cggaatcata
     2701 acattatttg caattggctt taatatactg gcaatattga attacaaaaa aacagtttag
     2761 taaattagga tgcagcataa ataattttaa ttattaatga tttggtataa atggctaaca
     2821 acagcacaaa actttttgaa gagatatttt cagaagagat aatttcaaac gaaagcttcc
     2881 gttccaaagt attggcagga attgttggtt tcctgatcat agttgtgctt ttaatttcat
     2941 taacatttga aaaacaattt gaagaaattt cccagttccc tatcgcattc cagttaacaa
     3001 taataatact ttcagtaata cttgtaagat cgctctttgt aagtagggct gcaaaacaat
     3061 ggacaagata cggagtcaag gctttcattc tgataagata tataaatgca tttgttgaaa
     3121 taagcatccc tacagttgcc ttaataattt attcatttaa cctgccatct gtgtttccat
     3181 tatttacgcc agttgcactt ctgtattttc taattataat gctatctgca ttggaacttg
     3241 attttaaact ttgtgtgttt tccggagccg tagctgctgt tcaatttact atacttgcat
     3301 ggtatttaag caataagcct ttaccaatcg aaacaataga ttccttttcg cttttcccgg
     3361 tttatctggg aaccgcagca ctgttgttta tttcaggtca tacagcagga cttataacta
     3421 atcaaataaa aaaaggattg attaaacact atcgtgcgca gtctgaaaga aatgaaatac
     3481 aaaaactttt tggacagcaa atctctaaag aaatagttga tgatcttgtt aaaaataaat
     3541 atgagataca aagccgtgtt cgatttgcta caataatgtt tttagatatc agaaactttt
     3601 caatcttcgc acaaaacaaa tcacctgaag aaataatagc atatcagaac aatgtattct
     3661 cttttataat tgaaatcata aatgagaata aagggatagt aacacagatt atgggcgatg
     3721 gattgatggc tattttcggg gctccgattg aacatgaaaa tgattgccag cttgctgtaa
     3781 atgcagcgct ggaaattaac aaagaactta aaagaagaaa taatgccggc ctgattcctg
     3841 aaacgattat tagaattggt attaatgccg gagaagtagt tacaggaaac gttggaacaa
     3901 gtgaaagaaa gcagtactct attacaggtc agcctgtaat tattgcagca cgacttgaac
     3961 aaattaataa agaattaaac tcagtaatat taatttcaga cagtgtatat aagcgtattc
     4021 ttttagctga agaacctaaa tatcataaag atcttgttat aaaaggtatt cccgatccaa
     4081 taacggtata tcaattagcg taaaaaaatt ttataaatca ttactctctt ttatttattt
     4141 ttgggggaca aaaataactt aagacttttt aatgcagcga ataaaaatcg atctaccgca
     4201 aaaatttctt ttcacaacag aaatatctgt aagagtttat gatgtaaact tcgcaggtca
     4261 tctcagtaat gattcaattc tttcaatggt gcatgaggcg agaattttat ttctgaaaaa
     4321 ttggggttat tctgaggtcg atacctgcgg cgcaggaata attatgtttg atgcggcact
     4381 tcaatataaa tcacagggct atcatggaga tattttacta tttcaagtaa cagtagataa
     4441 ttttattaag aatggctgcg atttcttttt caagattaca aacaaagcta cgggcaaaga
     4501 aattgcacgc gcaaaaacag gtatagcctt ttttgattac cagtctaata aactggttca
     4561 tgttccggaa gagtttagag cactgattga ttcacttaaa gtttaatttt aaaggctagc
     4621 aagttgtttg ataatatagt ttttacagca aacattgttg ccccggtatt tttaataatt
     4681 gcagttggat actttgctaa aagaacaaaa ataattaatg aagtttttgt agatgtaact
     4741 tcaaaatttg tctttcaaat ttcacttcct gttttcatct ttataaaaat ctctcaactt
     4801 gatttatcac aggcttttga gttcaatcaa atcatttata tttatattgg cacaatagtc
     4861 acctatctga ttatctgggc tgcgacaatt aaattt
//
LOCUS       DASWBY010000445         4022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_582999, whole genome shotgun sequence.
ACCESSION   DASWBY010000445 DASWBY010000000
VERSION     DASWBY010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582999"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..2848
                     /locus_tag="VF870_07970"
     CDS             56..2848
                     /locus_tag="VF870_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252163.1"
                     /translation="MKTILAVIFLTIFTNAQVQYTDMLKPQSIYTEAPTEVKSRKAFI
                     REWWFYEQRSFPNDFIPVDAYKNALDQRELLRQSNEQLALTDNSFNIPTFNWVSLGPT
                     SGAYFNYGNISSRIVTGAFDPTNANVIYIGPANGGVWKSTDAGISWMPLTDQQVSLSM
                     GAIEIDPTNSNIIYAGTGEATYSGASYYGRGLLKSTDAGNTWTNFTSGLPSLTYFSRI
                     KIRPSNSVQLLAALGNNGLYRSTNSGTSWTQIISGRIDDVAFTSSGDTVFAVGGSNGL
                     RRSIDGGATFSAFGSGLATGTRTHFDICLSNPAYMYAAVYGSIVNLYKSTNYGADWSQ
                     LTTTTEFQNQGGQAWYDLYIRVNPKNPNKVYVGTIDVYRSTDGTNFTNITNGYSGGNV
                     HVDQHFLFFHPTLENTFFVCNDGGIWKTTNNGDAFTNYNQYLTLTQFYRIAASPFTPS
                     RILGGTQDNGTQQTYSTLNWSAAYGGDGGEVCFNPFNSNFILGETQNGGLFRTTNGGS
                     SWSSAANGINTGENVAWVAPIIAHPTISGTFFVARQKVYRSTDNGGNWTAISANVNGS
                     TAVREMAISRSDPSILFATSSSSVFKSTDAGVTWTNVTSGLPSRTITSVSIYPTDPNQ
                     VFLTFSGFGSGKVYKSTNGGSSWISISGNLPDTPVNDIFIYSSNPGNPSTYFAATDVG
                     IFLTENDGTNWVEISNDLPNTVVMHLDYSPGNQMLRAGTHGRGVYEAFVDFSIPVELI
                     SFAANQQDKKVVLNWRTATETNNSHFEVERKFKNQDWEKLDIVAGAGTITEFRNYFYE
                     DDFESLPYNGTVLYRLKQVDYNGTFEYSNVISVDVEFIPTEISISQNYPNPFNPVTAI
                     KYWLNVEANVNISIYNSLGQVVEQLVNNIQSPGNYSVSWNAENYASGIYFYSFEVSDQ
                     NNSLLHKEMKKLIFLK"
     gene            3203..>4022
                     /locus_tag="VF870_07975"
     CDS             3203..>4022
                     /locus_tag="VF870_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9252164.1"
                     /translation="MQEEKLRLEDLLESSNSGNDDDRINNVAIIGAGIMGRGIAQTVA
                     SMGLEVVIIEVDSEKLESAKERLNANIDSEIKRWAMTKSDKKSIFSRIKWETDINAVK
                     DCEIIIEAVQEDFDSKVAIFKQLDEIVNPEAIFVSNTSTLSLTNISESISRPTKVIGM
                     HFLNPVPKVPMVELVKSLHTSNETVLKVKEFASRIGKTPVEVYEYPGFITTRAIVPLI
                     NEAMHILLEGIATAKDIDTALKLGYNFQYGPLEMADMMGLDEVLAWMETLWKTLG"
BASE COUNT         1340 a          667 c          784 g         1231 t
ORIGIN      
        1 gacgcaaata tgacgctcaa aatcaaccac tggagataat gaaagaatga atataatgaa
       61 aaccatccta gcagtgatat tcctgacaat atttacgaac gcacaagttc agtatactga
      121 tatgctcaaa ccccaaagta tttatactga agcgcccaca gaagtaaaat ctcgaaaggc
      181 atttattcgt gaatggtggt tttatgaaca gagatctttt ccaaatgatt ttattcctgt
      241 tgatgcatat aaaaatgcac ttgatcaaag agaacttctc agacagtcta acgaacaact
      301 tgcattaact gataacagtt ttaatattcc aacattcaat tgggttagtc tgggcccaac
      361 atccggtgca tattttaatt atggtaatat ttcaagccga attgttaccg gcgcttttga
      421 tccaacaaat gcaaatgtaa tttatatcgg tccggcaaat ggcggtgttt ggaaatcaac
      481 tgatgccgga attagctgga tgccgttaac tgatcagcaa gtctcgttat ctatgggtgc
      541 aattgagatt gatccgacaa attcaaatat tatttatgcc ggaacgggtg aagcaactta
      601 cagcggtgca tcttattatg gaaggggctt actcaaatcc acagatgcag gaaatacatg
      661 gaccaatttt actagtggat taccaagcct gacatacttt tcaagaatta aaataagacc
      721 cagcaattct gttcaacttc ttgccgcact cggtaataat ggattgtata gaagcacaaa
      781 ctctggaact tcctggacac aaataatatc cggaagaatc gacgatgttg cttttacttc
      841 ttctggtgat acagtatttg ctgttggcgg tagtaatggt ttaagaagat caattgatgg
      901 tggtgcaaca ttttctgcct ttggtagtgg attagcaact ggtacaagaa ctcattttga
      961 tatatgttta tcaaatccag cttatatgta tgcagcagtt tacggcagta tagtaaatct
     1021 ttataaatct acgaactatg gagcagactg gtctcagcta acaaccacaa cggaatttca
     1081 aaaccagggc gggcaagcct ggtatgattt gtatattaga gttaatccta aaaatcccaa
     1141 taaagtatat gttggaacaa ttgatgtgta tcgatctacc gatggaacaa attttacaaa
     1201 cataactaac ggatactccg gtggcaatgt acacgttgat cagcattttc ttttctttca
     1261 tccgaccctt gaaaatactt tttttgtctg caatgatggc ggtatctgga aaacaacaaa
     1321 taatggagat gcttttacaa attacaacca gtacttaacg ctaacacagt tttacagaat
     1381 tgctgctagc ccatttacac cgagtcgtat tcttggaggg acgcaggata atggaaccca
     1441 gcagacttac tcaacattaa attggtctgc tgcgtacgga ggagatggtg gagaagtctg
     1501 cttcaatcca tttaactcta attttattct tggtgaaaca cagaatggag gtttgtttcg
     1561 cactacaaac ggcggttcga gctggtcaag tgcagctaat ggaattaata caggtgagaa
     1621 tgttgcctgg gtagctccta ttattgcaca tccaacaatc tcaggaacat tctttgtagc
     1681 ccgccaaaaa gtttatagat caacagacaa cggaggaaac tggacggcaa tttctgcgaa
     1741 tgtaaatggc agcacagcag ttagagaaat ggcaataagt agatctgatc catcaatttt
     1801 gtttgcaact tcaagcagtt ctgtatttaa atctacagat gccggagtta cctggaccaa
     1861 tgttacatcc ggattaccaa gcaggacaat aacctctgtt tcaatttatc ctacagatcc
     1921 caaccaggtg tttttaacat tttccggttt tggttctggc aaagtttaca aatcaacaaa
     1981 tggcggaagc tcatggattt ctataagcgg caaccttcca gatacaccag tcaatgatat
     2041 ttttatatat tccagcaatc ctggcaatcc aagcacatat tttgctgcaa cagatgttgg
     2101 aatcttttta actgaaaacg atggaactaa ctgggttgaa atttcaaatg atcttccaaa
     2161 cacagttgta atgcatcttg attattcgcc cgggaatcaa atgttaagag caggtactca
     2221 tggaagagga gtttatgaag catttgttga ttttagtatt cctgtagagc tgatttcttt
     2281 tgcagctaat caacaagata aaaaagttgt tcttaattgg aggactgcta ctgaaacaaa
     2341 caatagccat tttgaggttg aaagaaaatt taagaatcag gattgggaaa aattggatat
     2401 tgttgcggga gcaggaacta taactgaatt tagaaattat ttttatgaag acgattttga
     2461 atcattacca tataatggaa ctgttcttta cagattaaag caggtagatt ataatggaac
     2521 attcgaatat tcaaacgtta tttcagttga tgtagaattt attcctacag aaatatctat
     2581 ttcacaaaac tatccgaatc catttaatcc cgtaacggca attaaatact ggcttaatgt
     2641 tgaagctaat gtaaacatta gtatttataa ttcgctagga caggttgttg aacagttagt
     2701 taataatata caaagtccag gaaattattc cgtaagttgg aatgctgaga actatgcttc
     2761 aggcatttac ttctactctt ttgaagtatc agatcaaaat aacagcttat tacataagga
     2821 gatgaagaag ctaatatttc ttaaataatt cagcttattt tcgtttgggg ctgtttttaa
     2881 gtttgattgg aaaaagttaa agattttaca ttttgataac caatcattct ttggacagcc
     2941 ccttttttat tgttaatttt ggttatgaaa aattgattca cttaagtatt aagatgaata
     3001 aagtaaatag aaaagatttt tgtccggtca aacacaattc ggttttatat ttaacaacag
     3061 gggatgataa ttttaaaact tcatcaactt gtagtaaata tttgacagcc attccatttc
     3121 gatcggatca tttttatcct attcttgttt aataaataaa aacaaatttt aaataaataa
     3181 aaagctttaa gtaaggattt ttatgcagga agagaaatta agattagaag atttactcga
     3241 atcttctaat tcaggtaacg acgacgacag aattaataat gttgccataa ttggagccgg
     3301 aattatgggg cgtggcatcg cacaaactgt tgcttctatg ggattggagg tagtgataat
     3361 tgaagtagat tctgaaaaat tagagagcgc caaagaaaga ttgaatgcta acatagacag
     3421 cgaaataaaa agatgggcga tgacaaagtc agataaaaaa tctatcttca gcaggataaa
     3481 atgggaaact gatattaacg ctgtaaagga ttgcgaaatt attatcgaag cggttcagga
     3541 agattttgat tcgaaggtag caatttttaa acaacttgat gaaattgtaa atccggaagc
     3601 tatatttgta tcaaacacgt ccacattaag cttaactaac atctccgaat ctatttccag
     3661 accaactaaa gttattggga tgcattttct gaatcctgtt ccgaaagtac caatggtgga
     3721 gttagtaaaa agtcttcata catccaatga aacagtttta aaggttaaag aatttgcttc
     3781 aagaatcggt aaaacacctg ttgaggttta tgaatatcct ggatttatta cgacacgcgc
     3841 gattgtacct ttgattaatg aagcaatgca tattcttctt gaaggaattg ccacggctaa
     3901 agatattgat acagcattaa agcttggtta caattttcaa tatggtccgc ttgaaatggc
     3961 agatatgatg ggattggatg aagtgttagc ctggatggaa actttgtgga aaacgctagg
     4021 tg
//
LOCUS       DASWBY010000446         3600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_204788, whole genome shotgun sequence.
ACCESSION   DASWBY010000446 DASWBY010000000
VERSION     DASWBY010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204788"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="VF870_07980"
     CDS             complement(<1..1691)
                     /locus_tag="VF870_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX9252165.1"
                     /translation="MKNLLLAVFVFLMAGTFVSQAQKLVDNFDSSAVNNVYLKINEGA
                     PTTMIYSDNHTDFQEGTGSFLADIKIGAFHSWGSYAEIQHASAEGEYLDFSGFDSLSI
                     WIKVYEPATAPANMVFRVQFRDQPSPSDPVEQYVYENAATLDAATGWFNLRISLGQIE
                     SDGSVNPNDQGFVITPSSWGLPKNNEILDYDKILSWGLVAVTTANAVDSVLVGFDDCT
                     MFSPKSLPAIVFNGIAYGPQIVQPTWAWGQSTMEVVTGAGPLPNTNAIKWVQGNEWNN
                     GWTGIGANVAPAFDLAVAWQQDSVQFKLKCEEGVGPLRMQFEGGGGKVGTVFTPTADN
                     QWHDYKLALRDMVYQDGTTGFDSSAVNVVGLMAEASGIAGKVIYITDWWTGNPEFDVV
                     PPSPPLNVFAFGGDKQNIITWDDVPGETGEKYDVYYSESPITDVANAEVVKFNIPENN
                     QLISHLLLSPNTDQSTTYYYAIVCKDASGNQSVVSVNSDAVTNTAKGVPTLSLNAPNF
                     AADGDLAEWQSITPFSMKISDGTGFIAPNTTISNDADLSVLSWVAVDANYLYVAI"
     gene            complement(1740..3368)
                     /locus_tag="VF870_07985"
     CDS             complement(1740..3368)
                     /locus_tag="VF870_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX9252166.1"
                     /translation="MSTFFSQIDLLVLVAYLIIVLSLGFYYSRRSDENYSDYFLAGRN
                     IGWFTVGISIFATNISSEHFIGLAGSGSTRGLAVGQFELMAIFTLIFLGWILAPIYLK
                     SGVVTVPEFLEKRFDRKIRKMFAGFSIAIYIFTKILVSLFAAGLLFYKIFGLNIYASS
                     IIIVLITGLYSVTGGANAVMRTQLFQGIILILGAVILSAFGLNAVGGFSGLEEKLPAD
                     FFNMFKAVNDPDFPWTGILFGAPIIAFWYWCTDQYIVQRLFSARSINDARRGTLLAAF
                     LKILPIFILVLPGLIAVALYPEIKGDEAYSALVAGDLLPIGLKGIVIAGLLAAIMSSL
                     AGAFNSTAVLFTNDFYKIRNPQANERKLVLVGRLTTTAVVVVAILIVPLVKIISSQIY
                     LFLQSVQAFVSPPITAVFLFGLFSKKINAKTALITLIVGETIGFSRLMLEIISNLGVQ
                     LHPILIWILSINFLHFAIFLFVVSITLILALNFSSVRIANDLTISLQYSISESIQELK
                     GNFVSIKTVRGLKSNFLLSAFIVMIIIGLWSIWY"
BASE COUNT         1226 a          706 c          581 g         1087 t
ORIGIN      
        1 tcaattgcta cgtataagta atttgcatca actgcaaccc atgataaaac tgaaagatca
       61 gcatcattag agattgtagt gttaggtgca ataaaaccag taccatctga tatcttcata
      121 ctgaatggtg taattgactg ccattctgca agatcaccat ctgctgcaaa gttcggtgca
      181 tttagtgaaa gtgtcggaac accttttgca gtattagtaa ctgcatcaga atttacactt
      241 acaacactct gatttccaga agcatcttta caaactatag catagtaata agttgtactc
      301 tggtctgtat ttggagataa taaaagatgt gaaattaatt gattattttc aggtatatta
      361 aatttcacaa cttcagcatt tgctacatct gtaattggag actcactgta atatacgtca
      421 tatttttcac cggtttcacc aggtacatca tcccaggtta ttatattttg tttatcgccg
      481 ccgaatgcaa aaacatttaa tggtggagat ggtggaacga catcaaattc aggatttcct
      541 gtccaccaat ctgtaatata aattactttt ccagcaatac cggaagcctc tgccattaaa
      601 ccaacaacat taacagctga tgaatcaaat ccagttgttc catcctgata gaccatgtct
      661 cttagtgcaa gtttataatc atgccattgg ttatcagcag tcggtgtaaa aactgtacca
      721 accttaccac cgccgccttc aaactgcatt cttaaaggtc caacaccttc ttcacatttt
      781 aatttaaatt gaacactatc ctgctgccag gcaactgcaa gatcgaatgc aggagcgaca
      841 tttgctccga tacctgtcca gccattattc cattcgttac cttgtaccca ttttattgcg
      901 ttagtatttg gtaagggacc agcaccggtt acaacttcca ttgtagattg accccaagcc
      961 catgtgggtt gaactatttg aggaccataa gcaatcccat taaaaacgat agctggtaaa
     1021 gattttggac tgaacatagt gcagtcatca aaaccaacta aaactgaatc aacagcattt
     1081 gctgttgtta ctgcaactaa gccccagctt agtattttgt cataatccaa tatctcattg
     1141 ttttttggta atccccagct tgaaggtgta ataacaaatc cctgatcatt ggggtttaca
     1201 cttccatctg attctatctg tcctaatgat attctaagat taaaccagcc tgtggcagca
     1261 tctaaagttg ctgcattttc gtaaacgtac tgttcaacag gatccgaagg agaaggttga
     1321 tctctgaatt gaactctgaa taccatattt gctggtgctg tagcaggctc gtaaactttt
     1381 atccagatac ttaaagaatc gaatcctgaa aaatccagat actcgccctc tgctgaagcg
     1441 tgctgtattt cagcatatga accccaacta tgaaaagcac cgatctttat atcggctaaa
     1501 aatgatcctg taccttcctg aaaatcagtg tgattatcag aatagatcat cgtggtggga
     1561 gctccctcgt tgatctttaa gtaaacattg ttcacagcag atgaatcgaa attatcgacc
     1621 aacttctgtg cttgagaaac aaatgtgcca gccatcagaa aaacaaaaac ggctaatagc
     1681 aaatttttca tactacctct ctctgtttgt ttttgaacca gttatttaaa tgtgtttttt
     1741 taataccaaa tactccaaag tccgataata atcattacaa taaatgcaga taacaaaaaa
     1801 ttagatttta atccgcgcac tgtttttatg cttacaaaat taccttttaa ttcctgaatg
     1861 ctttctgaaa ttgaatactg caagctaatt gttaaatcat ttgcaatccg aactgaagaa
     1921 aaatttaacg ctaatattaa ggttatgctt acaacaaaaa ggaaaattgc aaagtgaagg
     1981 aaattgatgc ttaatatcca gatgagtatc ggatgaagct gcacacccag attgcttata
     2041 atttctagca tcaaccgact aaacccaatt gtttctccta caatcaaagt aattagcgcc
     2101 gtttttgcat ttatcttttt cgaaaataat ccaaataaaa aaactgcagt tatcggcgga
     2161 ctaacaaacg cctgcacact ctgcagaaat aaataaattt gtgaactgat aattttaaca
     2221 agcggaacaa ttaatattgc aacaaccact actgctgttg ttgttaatct tccgactaaa
     2281 actaattttc tttcattggc ttgcggatta cggattttat aaaaatcatt tgtaaataga
     2341 acagcagtgc tgttaaaagc tccggctaaa gaagacataa ttgcggcaag taatccagca
     2401 ataacaattc cttttaaccc gataggaagt aaatcacctg caactaaagc tgaataagcc
     2461 tcatcacctt ttatttcagg ataaagtgct acggcaatta agcccggtaa aacaagaata
     2521 aaaattggta atattttaag aaatgctgct aacaatgttc cccttcttgc atcgttgatt
     2581 gatcttgcac tgaaaagtct ttgcacaatg tactgatcag tacaccaata ccagaaagca
     2641 ataataggcg caccaaacaa aatccctgtc catggaaaat ccggatcatt tactgctttg
     2701 aacatattaa aaaaatctgc aggaagtttt tcttctagtc cgctaaatcc gcctactgca
     2761 tttaaaccaa aagcagaaag gataaccgca cccaggataa gaataatacc ctggaaaagc
     2821 tgcgttctca ttacggcatt tgcaccgccg gtaacactgt aaagtccggt tataagtaca
     2881 ataattattg aagaagcata aatgttcaga ccgaaaattt tatagaataa taatcctgca
     2941 gcaaaaagtg acactaaaat ttttgtaaag atgtatatag caattgaaaa tcctgcaaac
     3001 attttccgga tttttctatc aaatcttttt tctaaaaact ctgggacagt cactacaccg
     3061 gatttaagat aaatcggggc aagaatccaa cctaaaaaga tgagagtaaa gattgccatc
     3121 aactcaaatt gtccaacagc tagtccacgt gttgaacctg atccggcaag accaataaag
     3181 tgttcacttg atatgtttgt tgcaaatatg gatatgccga ctgtaaacca accaatgttt
     3241 ctgccggcta aaaaataatc ggagtaattt tcatcacttc ggcgggaata ataaaagccc
     3301 aaacttaaaa caataattaa gtatgcgact aatactaata aatctatttg gctgaaaaat
     3361 gtagacactt ttgtataggc taatccatta aaaagcatta actaaacaca aataatgccg
     3421 tgtaattttg ataaaaaaat gttaaagaac attctttttg aagagttaaa aattttcttt
     3481 ggatttaagc ttattaaatt gataagtgtt atttattatt ttaataaagt ttagagtaaa
     3541 agaaatatca tctcataacc agaattctgt ataatcgcta aaaactattt gtacatttat
//
LOCUS       DASWBY010000447         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_544117, whole genome shotgun sequence.
ACCESSION   DASWBY010000447 DASWBY010000000
VERSION     DASWBY010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544117"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..2089
                     /gene="metG"
                     /locus_tag="VF870_07990"
     CDS             53..2089
                     /gene="metG"
                     /locus_tag="VF870_07990"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9252167.1"
                     /translation="MAKEKILVTSALPYANGPIHLGHLSGAYLPADIYVRYKRLNGDD
                     VIYICGSDEHGAAITISAEKEKVSPQVIIDRFHEANKNAFERFGMSFDNYSRTSIPIH
                     HETAIEFFLEFYNRGIFIKRKSLQFYDNQANIFLPDRYVEGTCPKCGNEQARSDECEN
                     CGSLYDPSELKNPKSKINGTTPALKETWHYYFPLGKYQPALEKYVDEMNEKYGWKSNV
                     LNYCRGWFKDGLNDRAITRDLDWGIKVPVDSAAGKVIFVWFEAVLGYISSTKELSQKK
                     NDPELWKKYWQDPKTKYIAFIGKDNVVFHTIIFPAILMAWNEGGKEQYCLPQNVPANE
                     FLNFEGKKFSKSRNWGIDVDDFLNLFAPDLLRYTLAANLPETRDTDFYWKEFQLRNNS
                     ELADILGNFINRTFTFVFKHFDGKVPALGKLENIDENMIQEISEYPARVADLFEKYKI
                     RDGVNEIMNLARDGNKYFNDSEPWKTVKSNKEHCGTTLNICLQTIYTLSELFSPILPF
                     STEKLFVMLNTQTVDWKDCGKPQLKEGHQLNQSEILFPKIEDEKIEAQINKLPKSEPE
                     KEKVELITYDDFMKIQLRTAEVIEAEKVPKSEKLLKLKVKLENEERQVIAGIAKSYQP
                     EDLIGKKVIIVANLQPAKLMGLESKGMILAVETKDGALQVVNIPQSVNNGTRVK"
     gene            2108..>3493
                     /locus_tag="VF870_07995"
     CDS             2108..>3493
                     /locus_tag="VF870_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9252168.1"
                     /translation="MKKLTFAFLCFGLITIFAQSPLEKIAPSLQLKMQSDVQSDKYLI
                     WVYFTDKGNSIDEYYANLQSVVSKKSLDRRAKVLSASNIIDYSDIPVNQNYITLLISK
                     GFELKQKSKWFNAVSGYASQNEINQLALIPFVKKIDIVGTYAKKIDDVELNSSEYLND
                     NPEQPEGINSLNYGNSFTQLNQINVPAVHDSGFTGAGVMICVMDAGFANLTHEAFSNM
                     NIAAMYDFVAGSPIMVGHSHGTATLSLIGGFKEGQLIGPAYGATFLLARTENDPGSET
                     PQEEDNWIAAMEWADSIGVDVTSTSLGYLEWDPPFTSYTWEDMDGNTARITIAADYAV
                     NLGITVVNSAGNDGYNSSHNTLGAPADGDSVITIGAINSSGIRAYFSSVGPTADGRIK
                     PDLMAMGEFNYYARTTGNLYGNGDGTSFSCPLVAGVCALLLQKNPALTPMEVLQLLRS
                     TATQSNSPDNLY"
BASE COUNT         1225 a          515 c          675 g         1078 t
ORIGIN      
        1 tttccttata tttaattttc aaaatcttta aaatattaat tcggttatta aagtggctaa
       61 agaaaaaata cttgtaacat ctgcacttcc ttatgcaaac ggaccaattc atcttggaca
      121 tttaagcggc gcatacttac cggcagatat atatgttcga tataaaagat taaatggtga
      181 tgatgtaatt tatatctgtg gttctgatga acatggagct gccataacaa taagtgcaga
      241 aaaagagaaa gtatctccac aagttattat agatcgtttt cacgaagcaa ataaaaatgc
      301 ttttgaaaga ttcggaatga gtttcgataa ttactcacga acaagtattc cgattcatca
      361 cgaaactgct atagaatttt tcttagagtt ttataatcgt ggaatattta ttaaaagaaa
      421 atctcttcag ttttacgata atcaagcaaa tattttctta cccgataggt atgttgaagg
      481 aacttgccca aagtgtggca atgaacaagc tcgaagtgat gagtgtgaaa attgcggttc
      541 actttacgac ccttcagagt taaaaaatcc taaaagtaaa ataaatggta caactccagc
      601 cttaaaggaa acttggcatt attattttcc tcttggtaaa taccaacctg cattagaaaa
      661 atatgttgat gaaatgaatg aaaaatatgg ttggaaaagt aatgtactta attactgtcg
      721 tggttggttt aaagatggat taaacgatcg cgctattacg cgcgatttag attggggaat
      781 caaagttccg gttgatagtg ctgcagggaa agttattttt gtatggtttg aagctgttct
      841 aggctacatt tcatctacaa aggaattatc ccagaaaaaa aatgatccag aattatggaa
      901 aaaatattgg caggatccga aaacaaaata cattgcattc attggtaaag ataatgttgt
      961 atttcataca ataatttttc ctgcaatttt aatggcatgg aacgaaggtg gaaaagaaca
     1021 atattgcttg ccgcaaaatg ttcctgctaa tgaatttctg aattttgaag gaaagaaatt
     1081 ttccaagagc agaaattggg gaattgatgt tgatgacttc ttaaatttgt ttgctccgga
     1141 tttattacgt tatacacttg cagcaaattt acctgaaacc cgtgacacgg atttttattg
     1201 gaaagagttt cagttaagaa ataacagtga acttgcagat attcttggaa actttatcaa
     1261 ccgtacattt acttttgtgt tcaaacattt tgatggtaaa gttcccgctc ttggaaaact
     1321 tgagaatatt gacgaaaata tgattcaaga aatttcagag tatcctgcac gagttgctga
     1381 tctgttcgag aagtataaaa ttcgcgatgg agtaaatgaa attatgaatc ttgcacgtga
     1441 cggcaataaa tattttaatg atagtgaacc ctggaaaact gtaaagtcaa ataaagagca
     1501 ttgcggtaca actttaaata tttgtttgca gactatttat acgttatcgg aattattctc
     1561 tcccattctt cctttttcaa cagaaaaatt gtttgttatg ttaaatacac aaaccgttga
     1621 ttggaaagac tgcggtaaac ctcaattaaa agaagggcac caattgaatc aaagtgaaat
     1681 tttatttcct aaaattgaag atgaaaagat agaagcgcag atcaataagc ttccaaaatc
     1741 agaacctgaa aaagaaaaag ttgagttgat tacttacgat gattttatga aaattcaact
     1801 tcgtaccgcg gaggtaatag aagctgaaaa agttccgaag agcgaaaaac ttttaaaact
     1861 aaaagtgaaa ttagaaaatg aagaaagaca ggtaattgca gggattgcaa aaagttacca
     1921 accggaagat ttgattggta aaaaagtaat tattgttgca aatcttcaac ctgcaaaact
     1981 aatgggactt gagtctaagg gtatgatttt agctgttgaa actaaggatg gcgcgttaca
     2041 agttgtaaat attccacaat cagttaataa cggaacaaga gttaaataat aatttatagg
     2101 aagataaatg aaaaaactta ctttcgcttt cttatgcttt ggattgatta caatatttgc
     2161 gcaaagtccg cttgaaaaaa ttgctccaag tcttcagtta aaaatgcaat cagatgttca
     2221 gtctgataaa tatttaatct gggtttattt tactgataaa gggaatagta ttgatgaata
     2281 ttatgcaaat cttcaaagtg ttgttagtaa aaaatccctt gatagaagag cgaaagtttt
     2341 atcggcatca aacataattg actattcgga tataccagtt aatcaaaatt acattacttt
     2401 attaatttca aaggggtttg aacttaaaca aaaatcaaaa tggtttaatg cagtaagtgg
     2461 ttatgcaagt caaaatgaaa taaatcagtt ggctttaatt ccttttgtaa aaaagataga
     2521 tattgtagga acatacgcaa aaaaaataga tgatgtcgaa ttgaattcct cagaatattt
     2581 aaatgataac cctgaacagc cagaaggaat aaattctctt aattatggaa attcatttac
     2641 tcaactaaat cagataaatg ttcctgctgt ccacgattca ggattcacag gtgcaggtgt
     2701 tatgatttgt gttatggatg cgggttttgc aaatttaaca cacgaagcat tctcaaatat
     2761 gaatattgct gctatgtacg attttgttgc cggcagtccg attatggttg gtcactcaca
     2821 tggtacagca acactttcac tcatcggtgg atttaaagaa ggtcaattaa ttggacctgc
     2881 ctatggtgca acatttcttt tagccagaac agaaaatgat cccggtagtg aaacaccgca
     2941 ggaagaagat aactggatag ccgcaatgga atgggctgat agtatcgggg ttgatgtaac
     3001 atctacttct ctgggttact tagagtggga tcctcctttt acaagttaca cgtgggaaga
     3061 tatggatgga aatactgcaa gaattactat tgcagctgat tatgctgtaa atctcggaat
     3121 tacagttgta aattctgcag gaaatgatgg ttataattcg agccataata ctttaggtgc
     3181 acccgccgat ggagatagtg ttattacaat aggtgcaatt aacagcagtg gtataagagc
     3241 atattttagc agtgtaggtc ctacagcaga tggaagaata aaaccagacc taatggcaat
     3301 gggtgaattt aattattatg cacgtacaac tggaaatttg tatggtaatg gtgatggtac
     3361 atcatttagc tgtcctttag ttgcgggtgt ttgcgcacta ctattgcaaa aaaatcctgc
     3421 tttaacacca atggaagttt tacagctttt aagatctact gccacccaaa gtaacagtcc
     3481 ggataatttg tac
//
LOCUS       DASWBY010000448         4077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_92148, whole genome shotgun sequence.
ACCESSION   DASWBY010000448 DASWBY010000000
VERSION     DASWBY010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92148"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..519)
                     /gene="mscL"
                     /locus_tag="VF870_08000"
     CDS             complement(121..519)
                     /gene="mscL"
                     /locus_tag="VF870_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX9252169.1"
                     /translation="MSMIKEFKEFAMRGNVVDMAVGIIIGGAFGKIISSFVGDILMPP
                     IGLLLGGMDFSNLTVTLKEGSEGVEPVLFKYGVFINTTIDFLIIAFAIFMVVKAMNSM
                     KKKKEEAPAAPPAPPADVQLLTEIRDLLKK"
     gene            complement(653..2224)
                     /locus_tag="VF870_08005"
     CDS             complement(653..2224)
                     /locus_tag="VF870_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252170.1"
                     /translation="MKKFLLPSVLVLSLMVVCASDIFSQWVPQTSGITTRLRYVYAVD
                     DNIVWACGNSGVILKTTDGGTTWATLTSPSATSTMYTLAAFDATTAWVTGTVGGSADV
                     SIWKTTDGGTTWSLQYNNPTGFGDGIKMFDANNGVYYGDPDPVTSSNWEILTTSNGGA
                     NWNRVPAGNYPPADSTGGEVGVACNLFTLGNTVWFSSYTSAGTIDFVYKSTNKGLNWT
                     RSSFQAISGGSSFISFKDQLNGIIVCLDNTIATTTDGGTTWTPSSVSGVGFRSIVNVP
                     EYESYVTVGSSGASHYTKDNGATWLPLTTGITSTLYWVTTSGNYCWAVGNSGVILKLN
                     GTVLPVELTAFTATVQNQKVTLNWTTATEINNNGFEVQRKVEGNEFITVGFVRGNGSS
                     TIQHEYSFVDNNLQNGIYTYRLKQIDYNGQFEYSNSIEVEVIIVGDYSLDQNYPNPFN
                     PSTKIGYTLKEKSNVKILIMNAIGEEIAVLVNQTQEQGYHQLDFNASNLPSGIYFYSL
                     QTENYSATKKMLLMK"
     gene            complement(2417..>4077)
                     /gene="pckA"
                     /locus_tag="VF870_08010"
     CDS             complement(2417..>4077)
                     /gene="pckA"
                     /locus_tag="VF870_08010"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX9252171.1"
                     /translation="GLVMKYLEFNTPATKQAMELASDFRLKNQGLTDLDRVFWNLPDE
                     ALYEEAIFRNEAKLSKQGPLVVNTGKHTARAAADKFIVQEQSTNDKIWWGAYNRPFSS
                     EKFNQLMGRVQAFCQGEELFVQDCYVGADPDYKMPIRIITEKAWHSLFARNMFITTED
                     RDELKKFVPDFTVICLPSFKLEAAIDGSRSETGIILNFAQRTAIIANSLYGGEIKKSV
                     FTLLNFLLTFEDVLPMHCSANVGKDGDVALFFGLSGTGKTTLSADPKRNLIGDDEHGW
                     SNEGVFNFEGGCYAKVIRLSAEAEPEIYATTRRFGTILENVVYDPVSRYIDLDDDQIT
                     ENTRCSYPLSFIPNVVPDGYVRTHPKNIIFLTCDASGVMPPIAKLNPAQAQYHFISGY
                     TSKIAGTEIGLGIEPQITFSACFGAPFMVRHPYEYAAMLKERMLKHKANVWLVNTGWV
                     GGRFGVGKRISIRHTRNLLNAALEGKLEKVKFRKDKLFGFEVPLTCPEVPEDVLEPSN
                     SWGNKDEYWKKYDALAARFVENFKLFEKDTSDEVKAAGPIRLSK"
BASE COUNT         1307 a          802 c          642 g         1326 t
ORIGIN      
        1 atgagaaagc cactaaagag gctaaagaac tcaccttaaa attctttaaa gataatttaa
       61 tgaaataaac aaaaaagcca tccaattagg gatggctttt tctatcactc tctgtttaaa
      121 ttactttttt aacaaatctc taatctcagt taaaagttga acatctgctg gtggagcagg
      181 cggtgcggct ggtgcttctt cttttttctt cttcattgaa ttcatagcct ttaccaccat
      241 aaatattgca aaagcgataa ttaagaaatc tattgttgta ttgataaaaa caccgtattt
      301 aaataataca ggttcaacac cttcagaacc ttctttcaat gtaacagtga gattggaaaa
      361 atccatccca cctaacaata atccaattgg aggcatcaaa atatcaccga caaatgaaga
      421 tataattttg ccgaaagctc cgccgatgat gataccaact gccatatcaa caacattacc
      481 gcgcatcgcg aattctttga attctttaat catactcatt ttgtatctcc tataatttga
      541 aatagttaag ttatttgagt tgtcgtccta aaataaacat aatcccaaat aaaaaaataa
      601 aaaaaaccgc agtgttaagc tgcggttttt aaagagctag aattaatgaa ttttacttca
      661 ttaacaacat cttctttgta gcagaataat tttctgtctg taacgagtag aaataaattc
      721 cgcttggaag atttgatgca ttaaaatcta actgatgata tccttgttcc tgtgtctgat
      781 taaccagaac agcaatttct tctccaattg cattcattat caaaatttta acatttgatt
      841 tttctttaag tgtatatcca atttttgttg atgggttaaa tggattcggg taattctggt
      901 cgagtgaata gtcacccaca ataattactt ctacttctat agagttagaa tactcaaact
      961 gaccattgta atctatttgt tttaatctgt atgtataaat tccattttgt aaattattat
     1021 caacaaaaga atattcatgc tgaatagttg agcttccatt acctctgaca aaaccaactg
     1081 taataaattc atttccttca acttttcgtt gaacttcaaa tccattatta tttatttcag
     1141 tagctgttgt ccaattaagc gttacttttt ggttttgaac tgtagctgta aatgcggtta
     1201 attcaacagg aagtacagtt ccattcaatt ttaatattac gccggaatta ccaactgccc
     1261 aacaataatt tcccgatgtg gtaacccagt ataaagtact tgttattcct gttgtcaatg
     1321 gtagccaagt tgcgccatta tcttttgtat aatgactagc accagaactt ccgaccgtga
     1381 catagctttc atattctggt acatttacaa tagatctaaa tccaacaccg gatacagaag
     1441 atggagtcca tgtagttcca ccatcagttg ttgtggcaat agtattatcc aggcatacta
     1501 taattccatt taattgatct ttgaatgata taaaacttga accacctgaa attgcctgaa
     1561 atgatgaacg agtccagttt aatcctttat tagttgattt ataaacaaaa tctattgtac
     1621 cggctgaagt atatgaacta aaccaaacag tgtttccaag agtgaataga ttacaagcaa
     1681 ctccaacttc accaccagta ctatcagcag gaggataatt tccagcaggt actctattcc
     1741 aatttgcgcc gccattggag gtagtcagaa tttcccaatt agatgatgta accggatcag
     1801 gatcgccata ataaacaccg ttatttgcat caaacatttt tatgccatca ccaaaacctg
     1861 taggattatt atattgtaat gaccaagtag tacccccatc agttgttttc cagatactaa
     1921 catctgctga accaccaacg gtacctgtta cccatgcagt cgttgcatca aaagcagcta
     1981 aagtgtacat tgttgatgtg gcacttggtg aggttaaagt agcccaagtt gttccaccat
     2041 cagttgtctt gagtataaca ccgctgtttc cacaagccca aacgatgtta tcatctactg
     2101 cgtagacata tcttaagcga gtggtaatac cacttgtttg aggaacccat tgtgaaaata
     2161 tatccgacgc acaaacaacc attagagata gaaccaaaac ggaaggaaga agaaattttt
     2221 tcataccgga ctcctgaatt gttttgagaa aattaaatta attagaacga gtgaaacctg
     2281 aacaattatt tattaaccgt gtttaaccta ataaatagaa tcttctttgt caatcagatt
     2341 aatgtaagta gaatatctga ttaaaagtgt agcaataaat aaaaaggtgg agtatatccg
     2401 ccttaataaa taataattac ttagataatc ttattggtcc ggctgctttt acttcatcgg
     2461 acgtatcctt ctcaaacagc ttaaagtttt ctacaaatct tgctgcaagt gcatcatact
     2521 tcttccagta ctcatcttta ttgccccacg agtttgatgg ttcaagtaca tcttcaggaa
     2581 cttcaggaca ggttaatgga acttcgaaac caaacagttt atcttttcta aactttactt
     2641 tttcaagctt tccttctaaa gcagcgttca aaagatttct tgtatgacga atactaattc
     2701 ttttgccaac tccgaatctt ccgccgaccc agcctgtatt tacaagccag acatttgctt
     2761 tatgttttaa cattctttct tttaacatcg cggcatattc ataaggatga cgaaccataa
     2821 acggcgcacc aaaacaagca gagaaagtta tctgtggttc tattcccaat ccgatttctg
     2881 ttccggcaat tttagaagtg tacccgctta taaaatgata ttgagcttgc gcaggattta
     2941 attttgctat tggaggcatc acgcctgaag catcgcaggt taagaaaata atattttttg
     3001 gatgtgttct tacatatcca tcaggaacaa cattagggat aaaacttaaa ggataagagc
     3061 atcttgtgtt ttcagttatt tgatcatcat ctaaatcaat gtatctgctt acaggatcgt
     3121 aaacaacatt ttctaatatt gttccgaatc ttcttgtagt tgcatatatc tcaggttcag
     3181 cttctgctga aagacgaata acttttgcat aacatccgcc ttcaaaatta aagacacctt
     3241 cattactcca gccatgttca tcatcgccaa ttagatttct tttgggatca gcagaaagag
     3301 tagttttacc tgttccgctt aatccgaaaa acaatgctac atctccgtct ttgccaacat
     3361 ttgcagaaca atgcattgga agaacatctt caaatgttag taaaaaattt agcagtgtga
     3421 atacagattt ttttatctca ccaccgtaaa gtgagtttgc aataatagca gtgcgctggg
     3481 caaagtttaa aatgattcct gtttcacttc ttgaaccatc tattgcagct tctaacttaa
     3541 aacttggcaa acagataaca gtaaaatcag gtacaaactt tttgagttcg tctctatctt
     3601 cagttgttat aaacatattt cttgcaaaca aactgtgcca tgctttttct gtaatgatac
     3661 gtataggcat tttataatca ggatcagcgc caacgtaaca atcctgaacg aataattctt
     3721 caccctggca aaaagcctga actctaccca tcaattgatt aaatttctct gaactgaaag
     3781 gacgattata agcgccccac cagattttat cattcgtgct ttgttcctga acaataaatt
     3841 tatctgctgc agctcgtgca gtatgtttgc ctgtatttac aacaagcgga ccttgtttag
     3901 aaagttttgc ttcattccga aaaattgctt cttcgtacaa agcttcatcc ggtaaattcc
     3961 agaacacacg atcaagatca gttaagccct gattttttaa tctgaaatca gaagcaagtt
     4021 ccattgcttg ctttgttgca ggagtattga attctaaata tttcatgacc aatccct
//
LOCUS       DASWBY010000449         4066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_168765, whole genome shotgun sequence.
ACCESSION   DASWBY010000449 DASWBY010000000
VERSION     DASWBY010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168765"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VF870_08015"
     CDS             <1..981
                     /locus_tag="VF870_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9252172.1"
                     /translation="LSDKLGSLKVMKFALYGSGVLFFAYSFITNYNWILVATFILAAV
                     NESFRPANLSMITEIVAPPQRRMAFALNRLAINAGMSIGPVIGGFLTLIDFHYLFYAN
                     AVASIAAGIYFSSIKWSSLAGSEKEKTTEEVSKIRFAILSDKSFLFFLFAVIPANLVF
                     FQHLGALPLYIVNDLGYSTAAFGLFGSINTILIIIAEVPLNNWMNDTPYKKSLMLGAL
                     FAGIGFGGFAIATTTFPLVIAIIVFTIGEMIFFPVTASYTSEIAPADRRGEYMGYYQM
                     TFSFAFSAGPWLGTVVYQNYGSEILWSGALVLGLISAVLMFFVKSNPTVK"
     gene            978..1694
                     /locus_tag="VF870_08020"
     CDS             978..1694
                     /locus_tag="VF870_08020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9252173.1"
                     /translation="MKEKLNKLILKLKQDKISNISTIGFIENNPITEVFERNNSYLIK
                     GTSDVDWVYVVCNNENYLKALLEKSSNNTYFASVEDWMIPVITEKRDPEWVLTTMRYY
                     LPDDVKVPKNKMEVIPLTTDHIGFIISQSDYKQFLTPKYVEERISKSISAAIMKKDKL
                     VAWGLTHDDGALGSLHVLDEYRKKGYGREILISLIHQNRKLGKVSFVQIEEKNTKAVN
                     LTEQLGFVKDRRVSWIKLLE"
     gene            1751..2200
                     /locus_tag="VF870_08025"
     CDS             1751..2200
                     /locus_tag="VF870_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252174.1"
                     /translation="MDLKELLKTQSGEILENALQLLNCAHLKSYSKSSQSDNKKRLLN
                     LLTLTEKCVIEKRLLPMKEYAAQIAKERFDAGFDLHEVHTAFNALEEELWNRVTKNIA
                     PENLGEALGLISTVLGAGKEELALTYVTLASKTRTPTLNLTELFGRN"
     gene            complement(2242..3594)
                     /locus_tag="VF870_08030"
     CDS             complement(2242..3594)
                     /locus_tag="VF870_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252175.1"
                     /translation="MKTRKVEQQIDYYITALNNSLIILRKFVEIRFHKLLLCSLIIPL
                     SLIFIHCTKENDEIINNPIDPGNTYQYTIEQAISDEAQRNTIAFDGLAFLTGNLGSQS
                     FLPPGKVADYSGFQCMRDNDPTQMGHNTDFVTIIAFNVLHILTTDQINMFVARAQNQI
                     NLINEFAYKRFPLLKAFRRLKEGDLPSGTTGLSKEAVKNYAAEFYKLDGLISYDRAKL
                     FGEVINSLSTSQKVSLDALKGFNGVGNWNKTLSDPLQGMNLPQDVNVAVMTYASEIYS
                     WYAGSVTSDVYFCPERHGTYFGSFYLKDWPAMGNPNYTIDETLTASAGQNFLSVLSQA
                     QAETIKGIVNLQRSALIELVAKREAISTELRKFLSSQIADSVTVINLSKQYGELDGEL
                     SYYYATTFTQVYNSLSDQQKGQVLNLSNQLGYINPTGAFLYSQPINMPEIINTDFLFD
                     "
     gene            complement(3656..>4066)
                     /locus_tag="VF870_08035"
     CDS             complement(3656..>4066)
                     /locus_tag="VF870_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX9252176.1"
                     /translation="QEYTCDGERATLPLELDGFPEDTKSFALIMHHEASSIDIHWYWV
                     LYDIPSSIHSLPKNVNGIGTLGNNSVNGKTEYAPPCSQGPGPKKYTYTIYSLSEAVNL
                     SVPNSEVSREVLLDAIKDITLASATLNVIYSRDI"
BASE COUNT         1318 a          690 c          716 g         1342 t
ORIGIN      
        1 ctttctgata aacttggttc cttaaaagta atgaagtttg ctttgtatgg ttcaggtgtt
       61 ctcttctttg cttactcttt tataacaaac tacaattgga tacttgtggc aacttttatt
      121 cttgccgcag taaacgagtc attccggcct gcaaaccttt caatgattac cgaaatcgta
      181 gctccgccgc aacgcagaat ggcttttgcg ttaaaccgac ttgcgataaa tgcagggatg
      241 agtattggtc ctgttattgg tggctttctt acattgattg attttcatta tctgttttat
      301 gcaaatgcag ttgcatcaat tgctgctgga atatatttca gttcaattaa atggtcctct
      361 ttagctggta gtgaaaaaga aaaaaccaca gaagaggttt caaaaattcg atttgctatt
      421 ctatctgaca aaagtttttt gttttttctt tttgcagtta ttcctgcaaa cttagttttc
      481 tttcagcatc ttggtgcgct tccattatat attgtaaatg atttaggata ttcaaccgca
      541 gcgtttggtt tgttcggatc aataaacact atcttaataa ttattgccga agttcctctt
      601 aacaattgga tgaacgatac gccatataaa aaatctttga tgcttggggc gttgtttgca
      661 ggaattggtt tcggaggatt tgcaattgcc acaacaacat ttcctcttgt aattgccatt
      721 atagttttca ccattggaga aatgattttc tttcctgtta cggcatccta cacttctgaa
      781 attgccccgg cagacaggcg cggtgagtat atgggatatt accagatgac tttcagtttt
      841 gctttttctg ccggtccgtg gttgggaaca gttgtttatc aaaactatgg ttctgaaatt
      901 ttgtggagcg gcgctttggt tttgggtttg atatctgcag tgttgatgtt ttttgtaaaa
      961 agtaatccta ccgtaaaatg aaagaaaaac taaacaaact gatattaaaa cttaagcagg
     1021 ataaaatctc caacatcagc acaataggat ttattgagaa caatccaatt acagaagttt
     1081 ttgagagaaa taattcatat ctcataaaag gcactagcga tgttgattgg gtgtatgttg
     1141 tttgtaataa cgaaaactat ctcaaagctt tgttagaaaa atcatccaac aacacctact
     1201 ttgcttctgt tgaagattgg atgattcccg ttattacaga aaaacgggac cctgagtggg
     1261 tattaacaac aatgcgctat tatctgccgg atgatgttaa agttcctaaa aacaaaatgg
     1321 aagtaatccc attaacaaca gatcatattg gatttataat atctcaatca gattataaac
     1381 aatttttaac tcccaaatat gttgaagaaa gaatttcaaa atccataagt gcggcgataa
     1441 tgaaaaaaga caaactagtt gcgtggggat taactcacga tgacggcgcg cttggttcac
     1501 ttcacgtgct ggatgaatac agaaaaaaag ggtacggaag agaaattctt atttctctga
     1561 ttcatcaaaa cagaaaactt ggcaaagtat cttttgttca aattgaagaa aaaaatacca
     1621 aagcagttaa tcttacagaa caacttggat ttgtaaaaga taggcgcgta agctggataa
     1681 aattattgga ataagttttt atctaaacct ttcttagttt tgaagtacaa attaattatt
     1741 ggtttataaa atggatttaa aagaattact caaaactcaa agcggagaaa ttttagaaaa
     1801 cgctttacaa ctattaaact gcgcgcatct taaatcatat tctaaatctt cacaaagtga
     1861 taacaaaaaa agattactaa atcttttaac attaacagaa aaatgtgtaa ttgaaaaaag
     1921 acttcttcct atgaaagaat atgcggcaca aattgctaaa gaaagatttg atgccggatt
     1981 tgatttacac gaagttcaca cagcatttaa tgcactcgaa gaagaactct ggaatagagt
     2041 aaccaaaaat attgctccgg aaaatcttgg cgaagcatta ggattgatca gcacagtact
     2101 tggcgcagga aaagaagagc tggcattaac ctatgttact cttgcaagca aaacaagaac
     2161 gccaacttta aatctaacag agctttttgg cagaaattaa ttttccggtt tctattatta
     2221 agattattgc ttttactctg cttaatcaaa taagaaatct gtatttataa tttcaggcat
     2281 attaattggc tgagagtaaa gaaaagctcc ggttggatta atgtagccta actgattaga
     2341 caaatttaat acttgccctt tttgctggtc tgagagagaa ttatatacct gagtaaatgt
     2401 agttgcataa taataactta attcaccatc aagctcgcca tactgttttg acaaattaat
     2461 tacagttaca gaatcagcta tttgtgaaga taaaaacttc cgcagttctg ttgaaatagc
     2521 ttctcttttt gcaacaagtt caataagagc acttcgttga agatttacta ttcccttaat
     2581 tgtttctgcc tgcgcttgtg aaagcacaga taaaaaattc tgacctgcac ttgccgtaag
     2641 tgtttcgtca atagtataat ttggatttcc catagcaggc caatctttta aataaaaaga
     2701 accaaaatat gttccgtgtc tttcaggaca aaagtaaaca tctgatgtaa ccgagccggc
     2761 ataccatgaa taaatttcac ttgcataagt cataactgca acattaacat cctgtggaag
     2821 attcattcct tgcagaggat cagataaagt tttattccaa ttaccaactc cattaaaccc
     2881 tttcaaagca tcaagactca ctttctgaga ggtacttaaa gaatttataa cttctccaaa
     2941 tagcttagct ctatcataac ttattaatcc atcaagttta taaaattcag ccgcataatt
     3001 ttttactgct tctttactta accctgttgt accagaagga agatcaccct cttttaatct
     3061 gcggaaggct tttagcaaag gaaatctttt atatgcaaac tcattaatta aattaatttg
     3121 attttgagct cttgcgacaa acatgtttat ttgatcggtg gtcagtatat gaagcacatt
     3181 aaaagcgatg atggttacaa aatctgtatt atgtcccatt tgagtcggat cattatcacg
     3241 catacattga aaaccgctgt agtcagctac tttacccgga ggaagaaatg attgtgaacc
     3301 aaggtttcct gtcaaaaatg ccagtccatc aaaagcaatt gtattccgct gggcttcatc
     3361 tgaaattgct tgttcaattg tgtattgata tgtattgccc ggatcaattg gattgttaat
     3421 aatctcatca ttttcctttg tgcaatgaat gaatattaat gataatggaa tgattagact
     3481 acataacaat aatttatgaa atctaatttc aacaaatttg cggagaatga ttaaagagtt
     3541 gtttaatgca gtgatgtaat aatctatttg ttgttctact tttctagttt tcataccgca
     3601 tgctttcttt ttattaatcg ttaagtaatt attttaacca caaaggtgta attttttaaa
     3661 tatcccgaga ataaataaca ttaagggtgg cacttgccag ggtgatatcc tttattgcat
     3721 caagtaaaac ttctctgctt acttcagaat ttggaactga aagatttaca gcttcggaaa
     3781 gcgaataaat tgtatatgtg tattttttag gaccgggccc ttgggaacaa ggaggggcgt
     3841 attctgtttt tccatttacg ctattattcc ccaaagttcc tattccgttt acatttttag
     3901 gtaaactatg aattgaactt ggaatatcgt aaagtaccca gtaccaatga atatcaatcg
     3961 aagaggcttc atggtgcatt attaatgcga aagattttgt gtcttcggga aatccatcca
     4021 attcaagagg aagagttgct ctttcaccat cacaagtata ttcttg
//
LOCUS       DASWBY010000450         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_168790, whole genome shotgun sequence.
ACCESSION   DASWBY010000450 DASWBY010000000
VERSION     DASWBY010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168790"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VF870_08040"
     CDS             <1..1192
                     /locus_tag="VF870_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9252177.1"
                     /translation="NEENRKMFIHAKTSKLNQTYFDPYVNMLRTTTEAFSAIVSGVDS
                     LQTSPFDESFNPADDFSKRIARNTQIILKEESHLDQVIDPAGGSYFVEKLTDEIATAA
                     WELFQKIDDDGGMLNAIKSGFVQNEIARVAEQKKKDFAKRKTVLVGTNMYANPKEEAI
                     ELKLIDYDSIYHQRVDYTKKYKMSNQVDERDQILRRLETLSDTSDFELIDTAIEAILD
                     GSTLGEVTKSIRATAEKGISVEPMQQFRLSEIFEELRIASDNYKKETGSKPKVFLATM
                     GPIKQFKGRADFSRSFFEVGGFEIIYPKGFATTDEAVKTALDSKAQVVVICSTDDTYP
                     ELVPPIAKGIKEKSKDVSVILAGYPKDQIEEHKKSGVDDFIYLGADAHKVLTTLLNKI
                     ITLN"
     gene            1305..>2264
                     /locus_tag="VF870_08045"
     CDS             1305..>2264
                     /locus_tag="VF870_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9252178.1"
                     /translation="MKPNFKDIKLDLNSKTISKKEWEEKFKQETGKSVEDFIWETMEK
                     IPVKPLYTKDDLQNLDHVDYLSGLPPFLRGPYSTMYVQRPWTVRQYAGFSTAEESNAF
                     YRRNLAQGQMGLSVAFDLATHRGYDSDYPRVVGDVGKAGVAIDSIADMKILFDQIPLD
                     KMSVSMTMNGAVLPVLAFYIVAAEEQGVKHEQLTGTIQNDILKEYMVRNTYIYPPEVS
                     MRIIADIFMFTAKNMPKFNSISISGYHMQEAGATADLEMAYTLADGLEYVRTGIKAGM
                     DIDEFAPRLSFFWAQGMNYFMEVAKMRAARLIWAKMIKQFNPKN"
BASE COUNT          797 a          381 c          430 g          656 t
ORIGIN      
        1 taatgaagaa aacagaaaaa tgtttattca cgcaaaaact tcaaaattaa accaaactta
       61 ttttgatcct tatgtgaata tgcttcgtac aacaaccgaa gcattttcag caatcgttag
      121 cggagttgat tcacttcaaa ccagtccttt tgatgaatca tttaatccag cagatgattt
      181 ttcaaaaaga atcgcccgta acacccaaat aattcttaaa gaggaatctc atcttgatca
      241 agtaattgat cctgctggtg gatcgtactt tgttgaaaaa cttacagatg aaattgcaac
      301 agcggcatgg gaattattcc aaaagatcga tgatgatgga ggtatgttaa atgctataaa
      361 atcgggattc gttcaaaatg aaattgcaag agtcgcagaa caaaagaaaa aagatttcgc
      421 aaaacggaaa acagtacttg ttggaactaa tatgtatgca aatcctaaag aagaagctat
      481 agaattaaaa ttaattgatt atgattcaat ttatcaccag cgtgttgatt atactaaaaa
      541 gtataaaatg tctaatcagg ttgatgaacg tgatcaaatt ttacgcagac ttgaaactct
      601 ttccgatacc tcagactttg aattgatcga tactgcgatc gaagctattc tcgatggatc
      661 aactcttgga gaagtaacaa aatctatccg ggcgactgca gaaaaaggta tttctgttga
      721 acctatgcaa cagttcagat tatcagaaat ttttgaagaa ttgagaatag cttctgataa
      781 ctataaaaag gaaacaggaa gcaaaccaaa agtatttctt gcaacgatgg gtccgataaa
      841 acaatttaaa ggcagagcag atttctcccg ctcatttttt gaggttggcg gatttgaaat
      901 catttaccca aaaggatttg caacaacgga tgaagcagtt aaaacagcat tggattcaaa
      961 agcacaagta gttgttattt gttcaactga tgatacttat cctgaacttg ttccaccgat
     1021 tgcgaaaggg ataaaagaaa aatcgaaaga tgtttcagta attcttgccg gttatcccaa
     1081 agatcaaatt gaagaacata aaaaatcagg agttgatgat tttatttatc tcggtgctga
     1141 tgcacataaa gttcttacaa ctttattaaa taaaattatc acattgaatt aaaaaccatt
     1201 gtcacactga gcgaagtcga agtgtgacct atttataaaa ataaaagaaa cgtcatcctt
     1261 agtcttcgct cagaatgaca tcgaaaaaca attttaagac ttttatgaaa cccaatttta
     1321 aagacataaa acttgatcta aattctaaaa caatctcaaa aaaagagtgg gaagaaaagt
     1381 ttaaacagga aaccggaaaa tctgttgagg attttatctg ggaaacaatg gaaaagattc
     1441 ccgtaaaacc actctacaca aaagatgatt tacaaaatct tgatcacgtt gactatcttt
     1501 ccggtcttcc cccatttttg cgcggaccct attcgacaat gtatgttcaa cgcccctgga
     1561 cagtgagaca atacgcggga ttttcaactg ctgaagaaag taatgctttt tatcgcagga
     1621 atcttgcaca aggacaaatg gggctttctg ttgcattcga tcttgcaaca catcgcggtt
     1681 acgattctga ttatccaaga gttgttggag atgttggaaa agcaggagtt gcaattgatt
     1741 caattgcaga tatgaaaatt ctttttgatc agattccgct tgataaaatg tctgtctcaa
     1801 tgaccatgaa tggtgcagtt ctacctgtgt tggcatttta catagttgct gcagaagaac
     1861 agggcgttaa acacgaacag cttaccggaa ctattcaaaa tgatatttta aaagagtata
     1921 tggttcgcaa tacttacatt tatccacctg aagtttcgat gagaattatt gctgatatat
     1981 ttatgttcac agctaagaac atgcctaagt ttaatagcat ttcaatttca ggatatcata
     2041 tgcaggaagc cggtgcaaca gcagacctgg aaatggcata tactcttgct gatggtttgg
     2101 aatatgttcg tactggaatt aaagcgggaa tggacattga tgaatttgcc ccacgtttat
     2161 cttttttctg ggcacaggga atgaactatt ttatggaagt agcaaagatg cgcgcagcaa
     2221 gattgatctg ggcaaagatg attaaacagt tcaatccaaa gaat
//
LOCUS       DASWBY010000451         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_696150, whole genome shotgun sequence.
ACCESSION   DASWBY010000451 DASWBY010000000
VERSION     DASWBY010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696150"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VF870_08050"
     CDS             <1..952
                     /locus_tag="VF870_08050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9252179.1"
                     /translation="NDIPIPEIGDNEVLVKVKVAAVNHLEMLIITGSVKLIQDYEFPL
                     TLGNELTGVVEKVGKNVRGYKVGDAIYSRLPLTKIGAFAEYAAINADAIGHLPANLDF
                     VTGAAAPLTGLTAYQGLHEELEAKAGETVFIPGGSGSFGQMAIPIAKAMGLKVIVSGN
                     PQARERTIEIGADQYIDYTTQDYWELLRDIDYVIDALGPDEFDHELSILKPGGRLLSL
                     RTGPNKRFAEEHGFSGWKQKLFAVAGFKNDNKAKKKGVQYRFIFVRSDGAQLEKLKKI
                     IEDNKIVPAVDPTEFRIEDIDKALNLVAQGHTKGKVVIRF"
     gene            1061..1906
                     /locus_tag="VF870_08055"
     CDS             1061..1906
                     /locus_tag="VF870_08055"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9252180.1"
                     /translation="MKINIPIEETIKNRISVRNYDSKRLSRSDKEKLVNEISQLTNPF
                     GENVRIHLIEKDTVSNGEKLGTYGVIKGANTFLGVTADKSEFGLVAAGYQFENLVLIA
                     TNMGLATVWLAATFSREQFEKAMDIREDELFPAISPIGYAAGKKSITESLMRKMMKSD
                     KRKPWDEIFFQDSFQNPLSRKDANEYVNPLEMLRLAPSATNAQPWRVVKKQGSYHFFE
                     IHKSNASEDEKMIKKVDLGIALSHFHQTVLEHGLVGCFDKLEDIKIDIPDNTNYIISW
                     NVEKK"
BASE COUNT          843 a          369 c          526 g          697 t
ORIGIN      
        1 taacgatatt ccaattcctg aaattggtga taatgaagtt ttagtaaagg ttaaagttgc
       61 agcagtaaat catttggaaa tgcttatcat aaccggtagc gtaaaactca ttcaggatta
      121 tgagttcccg ttgacacttg gcaatgaact tacgggtgtt gttgaaaagg ttggtaaaaa
      181 tgttcgaggt tataaggtag gagatgccat atattcaaga cttcctttaa caaaaatcgg
      241 agcttttgct gagtatgcgg caataaatgc agatgctatt ggacatttgc cggctaatct
      301 tgattttgta accggtgctg cggcaccatt aacaggattg acggcttacc aggggttaca
      361 tgaagaattg gaagctaaag ccggtgaaac tgtgtttatt ccagggggtt ccgggtcatt
      421 tggacaaatg gcaattccaa ttgccaaggc aatgggactt aaggtgattg tcagtggaaa
      481 tccacaggca cgtgagcgga caattgagat aggtgcagac caatatatcg actacacaac
      541 tcaagattat tgggaactgc ttagagacat agattatgtg atcgatgccc taggaccaga
      601 tgaatttgat catgaacttt ccattcttaa accgggcggc aggcttcttt ctttgcgcac
      661 cggtcctaac aagcggtttg cagaagaaca tggattttca ggttggaaac aaaagctctt
      721 tgctgttgcg gggtttaaaa atgataataa agctaagaaa aaaggggttc aatatcgttt
      781 tattttcgtt cgaagtgacg gagcacaatt agaaaagctt aaaaaaatta tcgaagataa
      841 taagattgtg ccggctgtag acccgacaga atttcgtatt gaggacattg ataaagcact
      901 gaatctcgta gcgcaaggtc atacaaaagg aaaagttgtc atcagatttt aagaattagc
      961 tatgtagatt cgtaatttct gtgatacatt tcaattgagt aagaaaaagc cataggtggc
     1021 ttgatatttc aagaaagata gataaagtga ggtagaaaga atgaaaatta atattccaat
     1081 agaagaaacg attaaaaaca gaataagtgt aagaaattat gattcaaaaa gactttcaag
     1141 aagtgacaaa gaaaagttag tgaatgagat aagtcagttg acgaatccat ttggagaaaa
     1201 tgttcgaatt catcttattg aaaaagatac agtatcaaat ggagaaaaac taggtacata
     1261 tggtgtgatt aaaggagcca atacatttct gggtgtaact gctgataaaa gtgagtttgg
     1321 acttgttgct gccggatatc agtttgagaa tctggtactg attgccacta atatgggact
     1381 tgcaactgta tggcttgcag ctacctttag cagagagcag ttcgagaaag ccatggacat
     1441 acgggaagat gaactgtttc cggcaatttc accgattgga tatgcagccg gtaaaaagag
     1501 cattacagaa agtctaatga gaaaaatgat gaagtcggat aaacggaaac catgggatga
     1561 aattttcttt caggattcat ttcaaaatcc actttcaagg aaagatgcaa atgagtatgt
     1621 aaatcctttg gagatgcttc gattggcacc ttctgctacc aatgcacaac catggagagt
     1681 ggtaaaaaaa caaggctcat atcatttttt tgagatacac aaaagcaatg caagtgaaga
     1741 tgaaaaaatg attaaaaaag tggatcttgg aattgcttta agtcatttcc atcagacagt
     1801 attagaacat ggattagtag gatgctttga taaactggag gatataaaaa tagatattcc
     1861 ggataataca aattatataa tatcatggaa cgtggaaaaa aagtaaaacg atgaatgcca
     1921 tagcttttat tgatgaaaat atcaatgctg tccactcgag ccatgttgag tgcgttgaga
     1981 ggatagagcg gaaataggtt ggataacgaa aaaccctaat ggaatagtaa taatcaaata
     2041 aaaaaacaat tcgctatgta gtaaagcgaa ttgttttttt tataaaagga gttcacaagt
     2101 tattttacct aaaaacagag attatccgat tcgctaccaa taactgtaag atgtctgtcg
     2161 cctttcaaaa tataaaactt tggctacatt ggtatagaag gcagttagat gccttcattt
     2221 ttttttattt accaataaaa gtagccattc cattatcacc actctaagga ggggctcctt
     2281 taactgttac ataacaaatt attagcatat actctgctcc tctaggaatg gctcgattga
     2341 aagttaatat aaatgtttta gctgagccat tttttgactc tattctaacc atattacccg
     2401 taagtgagtt tccggaattt acactatcgt tttta
//
LOCUS       DASWBY010000452         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_583256, whole genome shotgun sequence.
ACCESSION   DASWBY010000452 DASWBY010000000
VERSION     DASWBY010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583256"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..946
                     /gene="prmA"
                     /locus_tag="VF870_08060"
     CDS             101..946
                     /gene="prmA"
                     /locus_tag="VF870_08060"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX9252181.1"
                     /translation="MKKSYKEYIITAEPFNVEILSSILWELNIDGINEEVNCLKVFAQ
                     DELVTIQTIENELTHLKENKLLREFSVQENFIVEKNWNEEWEKSREVVQVSDKIIIKP
                     TFKEYEAKSGEIVLTLDPKMSFGTGDHQTTKICLIFIEKYLNPGMKVLDAGSGTAILG
                     IAAAKLGAEKVIAFDIDEWCFDNGIENTKLNYVGHKVEIRKCELKDIDEKDFDLIIAN
                     IQKNILLELADGFKSRIKQNGILILSGLLEMDREAIKQKYSLNGFKEIDFMQMDDWIG
                     VVFKK"
     gene            complement(995..1390)
                     /locus_tag="VF870_08065"
     CDS             complement(995..1390)
                     /locus_tag="VF870_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252182.1"
                     /translation="MKKFHFVLFVFFCLSVFGSINQTYAQEIYFCEGVDDDGYPINAS
                     TNFTIPEDGGYLYVLVRLPNKVNCRTVKLLIYNEDEELENTITIDTERSWTWFWKKVA
                     FYEAGEFIVDVWDCNTEYLVTGNIEIDKD"
     gene            complement(1481..>2140)
                     /locus_tag="VF870_08070"
     CDS             complement(1481..>2140)
                     /locus_tag="VF870_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9252183.1"
                     /translation="TNGEMEKWCLDADKNHLQFSVHAIGDKANAWMLDLFEKITKENP
                     KWDRRFRLEHAQHVRFEDIKRFAQLGVIASVQPYHCIDDGVWAEKRIGPERIKYTYPF
                     KSFLDAGVKMCFGTDWYVAPLNPLLGIYAAVTRRTLDDKNPNGWIPEQKISVEDAIKC
                     YTLNSAYASFEENIKGSIEVGKLADLIILSDDILSIDPVKIKDAQVEVTMFDGKIIYS
                     K"
BASE COUNT          762 a          343 c          334 g          701 t
ORIGIN      
        1 caagccagag atttacagga tgaaatctgt aaaattttgg cggattataa ataagtcaca
       61 ctgagcgaag ccgaatggtg acctctgaat taggacatca atgaaaaaat catacaaaga
      121 atatattata actgcggaac catttaatgt cgaaattcta tcttcgattt tatgggaact
      181 taacatcgat ggaattaatg aggaagtaaa ttgccttaaa gtttttgctc aggacgaact
      241 ggttacaatt caaacaatcg aaaatgagtt aacacatctt aaagaaaata aattattgcg
      301 ggaattttcg gtacaggaaa attttattgt tgagaaaaac tggaatgaag aatgggaaaa
      361 gagcagggaa gttgtacagg tttccgataa aattattatc aaaccaacct ttaaagaata
      421 tgaagcaaaa tccggtgaaa ttgttttaac attagaccca aaaatgtcat tcggtacagg
      481 tgatcatcaa acaacaaaaa tatgtttaat ctttattgaa aaatatttaa acccgggaat
      541 gaaagtttta gatgcaggct ccggtacagc gatacttggc attgcagcgg caaaacttgg
      601 agcagaaaaa gtaattgctt ttgatattga tgaatggtgt tttgataatg ggattgaaaa
      661 cacaaagttg aattatgttg gccataaagt tgagattaga aaatgtgagt taaaagatat
      721 tgatgaaaaa gattttgatt taattatagc taatatccaa aaaaatattt tgttagaact
      781 tgctgatgga tttaaatccc ggataaaaca aaatggtatt ttaatccttt ccggtttact
      841 tgaaatggat agggaagcaa ttaaacaaaa atattcttta aatggattta aagaaattga
      901 ttttatgcaa atggatgatt ggattggagt tgtatttaaa aaataaaacc cagttaaaaa
      961 taactgggtt tttagaaaaa tgatttaata ttctttaatc tttatcaatc tcaatatttc
     1021 ctgttactaa atattctgta ttacaatccc aaacatccac aataaattca ccagcttcgt
     1081 agaatgcaac ttttttccaa aaccaggtcc agcttctctc tgtatcaatc gtaatagtat
     1141 tttctaattc ttcatcttca ttgtaaatca gaagtttaac tgtacgacag tttactttat
     1201 ttggtagtct taccaaaaca tatagatacc cgccatcttc aggtatggtg aaatttgtac
     1261 ttgcatttat agggtaacca tcatcatcaa caccttcgca aaaataaatt tcctgcgcgt
     1321 atgtttggtt aattgaacca aatactgaaa gacaaaaaaa tacaaagagc acaaagtgaa
     1381 attttttcat atataacctt tggattagtg gtgggaaaaa ctaaaaacaa tattttatat
     1441 ttcaaaagct tagaaaaaaa atattatttt atagtagaaa ttatttgctg taaataattt
     1501 tcccatcaaa catcgtcact tcaacttgcg catcttttat cttaaccgga tcgatactta
     1561 aaatatcatc actcaaaata attaaatctg caagtttgcc aacttcaatg cttcctttaa
     1621 tattctcttc aaatgatgca taagctgaat ttagggtata acatttaatt gcatcttcaa
     1681 cagaaatttt ttgttctggt atccatccat ttggattttt atcatccaat gttctgcgtg
     1741 taaccgcagc ataaatccct agtaatggat ttaatggagc aacataccaa tcagtaccaa
     1801 aacacatttt tactcctgca tccaaaaaag atttgaacgg atatgtgtat ttaattcttt
     1861 caggtcctat ccttttttca gcccaaacac catcatcaat gcagtgatac ggctgtacag
     1921 aagctataac gccaagttgt gcaaatcgtt ttatatcctc aaatcttaca tgttgcgcgt
     1981 gttcaagtct aaatcttcta tcccattttg gattttcttt agtaattttt tcaaatagat
     2041 ccagcatcca ggcattagct ttatctccaa tagcatgtac agaaaactgc aagtgatttt
     2101 tatcagcatc taaacaccac ttttccattt ctccgtttgt
//
LOCUS       DASWBY010000453         6881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_21335, whole genome shotgun sequence.
ACCESSION   DASWBY010000453 DASWBY010000000
VERSION     DASWBY010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21335"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VF870_08075"
     CDS             complement(<1..261)
                     /locus_tag="VF870_08075"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9252184.1"
                     /translation="MFPKLYKIFLLVLFACSFTFSQTGRISGKVIDQQTGEALIGANI
                     IIVGTSLGAASDINGEYLVTNLNAGQYSVKASYIGYQDMTVNY"
     gene            complement(293..1006)
                     /locus_tag="VF870_08080"
     CDS             complement(293..1006)
                     /locus_tag="VF870_08080"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX9252185.1"
                     /translation="MRIPYKRLLVAINICAVVFITGCVDTDVNPLPPSIDYQSQMKVV
                     NLVQGAGTATLTLNGQSLGTVDFGSETPGSSAAFLTVPSGSKSVDASFTTAANQNYRF
                     AATTEYKMRVFLVGTSAASNAFVQFQRYIWQTKDSQNGAALFPADTGWVAFFNGSPDA
                     TISTVEVNGEDIHLNSIVTGSGSSYVKLPAGTHTFDVYYNDDADHITFDIDVASKGRY
                     TATIYDVAASIKNAVFVDD"
     gene            complement(1547..3091)
                     /locus_tag="VF870_08085"
     CDS             complement(1547..3091)
                     /locus_tag="VF870_08085"
                     /inference="COORDINATES: protein motif:HMM:NF039629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HEX9252186.1"
                     /translation="MKLSSYHLILLFILITSARIFSQSSSNWFLLPDGLGLQNQFEYS
                     YNVENKIEILENWLTLDYNKNIFSAGLRFEVFQPNDPDPSISRGKVKYADIAYKYISA
                     DIGDVEEGLNITVGNYYKLFGRGLVLKSYEDRNIRVDNNLIGVKVEGNYLGFSLIGLS
                     GSAANSNNERQDILHAVDLSYNNLSFIKPGITFATNLPPIEGIANTSLASFRVQPTYW
                     NLDAYAEYGIKSNKDIQSQIFNNDEWKIGEAFYGSLNFYYYAFAIVGEYKYYDNYAFT
                     SNDGTIFYNTAPSLRKDYTYVLLNRHPSPLNESNEEGYAFEVNYNFSDETSLIANYGI
                     TNTLPANSYYQRVNNLSLPISKQLEEVYLQATQNWNESFTTIAAFGYNEELSTNTKNI
                     TPILENKFYFDDINTIKLVFEHQQTENLSTNEKYYDDVINIEYLRSPNFSVTVVAELQ
                     TKEPEPGNVVRKFWSFVQFGYKLGNHTDLSLLLGTRQAGNICIGGICRYEPAFQGVEI
                     KMLTRL"
     gene            complement(3166..3669)
                     /locus_tag="VF870_08090"
     CDS             complement(3166..3669)
                     /locus_tag="VF870_08090"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9252187.1"
                     /translation="MRLVFLMVFTLFISLPLIAQNEEEITGRKAPNFKLVNLDGKYVE
                     LNKEAGNGPILLSFWATWCKPCLEEMAEYNKIYEQYKDKGFSLLAISTDNEKSVAKVK
                     PYIKSKGYNFSVLFDTNSEVARKYYAQQMPYTIIIDKSGNIVYSHLGYMKGDEQKVEK
                     LITELLE"
     gene            complement(3716..4441)
                     /locus_tag="VF870_08095"
     CDS             complement(3716..4441)
                     /locus_tag="VF870_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252188.1"
                     /translation="MIKELLSFFMVFLLTGSIFSQDITFVPRETVLYDTLGSEMIFYI
                     DVTNITQADQTLFMVRTENNLPASWQSSLCFEYCFPGWVDSVATNSDFGSTPLAPNET
                     REVAIHVFPLVNLGTGYVQMQAATFRNPSSRVTVDFTAIVNPTSVEDENTVVDNYYLA
                     QNYPNPFNPSTRINFGLKNPGNVEISVYNILGNKVATLVNGYKPAGRHSVVFNAYSLS
                     SGTYFYKIVTNEFTLTKKMLMLK"
     gene            complement(4503..6797)
                     /locus_tag="VF870_08100"
     CDS             complement(4503..6797)
                     /locus_tag="VF870_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252189.1"
                     /translation="MKKATLFFLSWFAALSVFTFAQVQLKFVTINGYGNNLYIDNVTV
                     GNQFNLDAAVGSINNINSGTSYATSTLPFVITPNVSVINIGKQNITTSFNVTMTATPG
                     GYTSTKTITSLNSGQSTNVTFDDLTITPGQGIDISVVTNLAGDENSTNNTLNQYTIYL
                     PGTFRNILLEEWTSSTCGPCAANNPTIDAFVAARFDSIVPIKYHMNWPSPGNDPMYLY
                     NPTQATDRRSYYGVNAVPHVIMDGVVDPSYPYSDPTSLPGAFNPRKKVGTPIAMSVTD
                     TRISNGDSVRADITVQVLSPLNPGSYYLRVHAVERHIKYATAPGTNGEKDFYDVFRKA
                     YPNSLGTSVPTGVGTYNYTITYAIDKAVWVDSMIYTAAFVQNDQTKEVLNSAKGRNEV
                     LENMIVNNQNTIIEKPVSASDFVVQSKPASVVVNTDGINSVFNYELFEGSFPANGWTL
                     KNPDGGITFSQFTGANGPSIAGSKSVKMDFYSYSTTGASDTLISKVFNGAEVTDSVKF
                     NYAHANYPGYTDRLVVKLSVDGGLTFPHTIFDKSGAALATVPASTNAFVPSSASQWAT
                     FSYSLESVLPVELKSFTAKVVGSSVHLNWSTATEVNNLGFEIQKKVAGEFVAVGFING
                     KGTTTEVQNYSYTDNNLQNGSYTYRLKQVDLTGSFEYSEEVNVDVIGPQYFSLKQNYP
                     NPFNPSTRIEFNLASESKVTLKVFNLLGEEVVTILNGNLASGNHSVDFRAQNLNSGVY
                     IYRLDAAGIDGSVYSDIKKMTLIK"
BASE COUNT         2213 a         1226 c         1122 g         2320 t
ORIGIN      
        1 ataattaact gtcatatcct gataaccaat gtaagaagcc ttaacgctat attggcccgc
       61 attaaggttc gtaacaagat attctccatt aatatctgaa gccgcgccaa ggcttgtgcc
      121 cacaattatt atattcgccc caatcagggc ttcgcctgtc tgctggtcaa taacttttcc
      181 cgaaatacgt ccagtttgag aaaaagtaaa agagcaggca aacagtacta aaagaaaaat
      241 tttgtataat ttgggaaaca tttttttcct ccgttaatta ttaactattt tattaatcat
      301 ctacaaaaac tgcattttta atacttgctg caacgtcata aatagttgca gtatacctgc
      361 ctttagaggc aacatcaata tcgaaagtta tatggtcagc atcatcatta tagtacacat
      421 caaatgtgtg tgtgccagca ggtaatttaa cataggatga gccgctgccg gtgactattg
      481 aattaagatg aatgtcttcg ccattaactt ctactgtgct gatagttgca tcaggggatc
      541 cgttaaaaaa tgcaacccaa ccagtatctg ccgggaacaa tgctgctcca ttctgtgaat
      601 ccttagtttg ccaaatgtat ctttgaaact gtacaaaagc attacttgct gctgatgttc
      661 caactagaaa aactctcatt ttgtactcgg ttgtcgcagc aaaccgatag ttttgatttg
      721 cggcagtcgt aaaactagcg tccacagatt tactgccgga aggaacagtt aaaaatgctg
      781 cagaggagcc aggtgtttcg ctcccaaaat caactgtgcc aagcgattga ccattcaaag
      841 tgagagtggc agtacccgca ccttgtacta ggttaacaac tttcatttgg ctttgataat
      901 caatactggg gggaagagga tttacatctg tatcaacaca gccagtaata aaaaccacag
      961 cgcaaatgtt aattgctact agtagtcttt tgtaaggtat tctcatttta cctcctagta
     1021 aaataattga tataattaag ttgagaaaca tttaatagat aaataatatg gttcatagtt
     1081 caaatcaata tttagtaatt catcaaattt aattttatta cattataaat gaacttataa
     1141 ataataacag caggtatttt tatcagtcct ttgtttttat gttaattaat ttggtttaac
     1201 taacattatg caatacacaa attttgaaag aacaagctta actatttaaa ctgagtatga
     1261 aatattttga taatttagaa taataaggga tgatattcgt tctgagagca taactatcca
     1321 tcaaaatatc cgaagaagct ttggatattt ctaacgtaat ggatataaga gagttaaaca
     1381 aatttgtatc aattattctg ataaacattc cttatttaaa agatttctaa aagtcatatg
     1441 tgaacgtcct aaaaaatatc attaaagtca acattttttt aaaaatgttt tattttttta
     1501 attatttgca attaacaagt tttcaactaa agtataaaaa tatttttcat aatcttgtta
     1561 acattttgat ttcaaccccc tgaaatgcgg gctcatatct gcatatcccg ccgatacaaa
     1621 tatttccggc ttgtcttgta cccaaaagca ggctaaggtc tgtgtggttg ccaagcttat
     1681 agccaaactg aacaaagctc caaaattttc ttacaacgtt tcccggctcg ggttctttgg
     1741 tttgtaattc tgccacaaca gtcacgctaa aatttggcga gcgcagatat tcaatattaa
     1801 ttacatcgtc ataatacttt tcattcgttg aaagattttc tgtctgctga tgttcaaaca
     1861 ctagtttaat tgtattaatg tcatcaaagt aaaatttgtt ttctaaaatt ggtgttatgt
     1921 ttttcgtatt tgttgataac tcttcattat aaccaaatgc agcaattgtt gtaaatgatt
     1981 cattccagtt ttgtgttgcc tgcagataga cttcttctaa ttgttttgat atcggaagtg
     2041 aaaggttatt tactctttgg taatatgaat ttgcaggcaa tgtgttggtg atgccataat
     2101 ttgcaattaa ggaagtttca tcagaaaaat tataattaac ctcaaaagca taaccttctt
     2161 cgttagattc attaagcgga gaagggtggc gatttaaaag aacataggta taatcttttc
     2221 ttaaggaagg cgcagtatta taaaatattg ttccgtcgtt ggaagtaaag gcataattat
     2281 catagtattt atattcgcct actattgcga atgcataata ataaaaattt aaactgccgt
     2341 aaaaagcttc gccgattttc cactcatcat tattaaatat ttgtgactga atatctttat
     2401 tgcttttaat tccgtactca gcatatgcat caagattcca gtacgtgggt tgaactctaa
     2461 atgaagctaa agaagtattt gcaattcctt caatcggcgg cagattggtt gcaaaagtaa
     2521 ttccaggttt aataaaactt aagttattat agctcaaatc aacggcgtgc aatatatcct
     2581 gtcgttcatt atttgaattt gcggcagagc cggataatcc gattaacgaa aaccctaagt
     2641 aattaccttc tacttttact cctattaaat tattgtcaac tcgtatgttt ctgtcctcat
     2701 aacttttaag cactaatcca cgaccaaaaa gtttataata attaccaacc gttatattta
     2761 atccctcttc aacatctccg atatcagcag aaatatattt atatgctata tcggcatatt
     2821 taacctttcc cctgctaata gaaggatcgg gatcattcgg ctggaaaact tcaaatctca
     2881 atcctgcact aaaaatattt ttattataat ccagagttaa ccagttttca agaatctcaa
     2941 ttttattttc aacattatag gaatattcaa attgattttg taatcccaaa ccatcaggta
     3001 ataaaaacca gttactgctg ctctggctaa aaattcttgc tgatgtaatt aaaataaata
     3061 ataaaataag atggtaactt gataacttca tttttcgatc tgaaataatt ttatgataaa
     3121 tattgtctgc ttcgaattca ttttatccga agggtttcgg aattattatt ctaacaattc
     3181 ggtaattaat ttttcaactt tctgttcgtc acctttcata tagccaagat gcgagtaaac
     3241 aatgtttccg gatttgtcta ttataatagt ataaggcatt tgctgtgcat aatattttct
     3301 tgcaacttca ctgttagtat caaacaaaac agaaaaatta tatccttttg attttatata
     3361 tggttttact tttgcaacgg atttttcatt atcggttgaa atggcaagta atgaaaatcc
     3421 tttatcttta tattgttcat aaattttatt gtactcagcc atttcttcca aacaaggttt
     3481 acaccaggtt gcccaaaaac ttaaaagtat aggtccatta cctgcttctt tattcagttc
     3541 aacatatttc ccatcaagat taacaagttt aaagttgggg gcctttcttc cagtaatctc
     3601 ttcttcgttt tgtgcgatta atggaagcga aataaaaagc gtaaaaacca tcaaaaagac
     3661 taatctcata aatttctccc gtatccagat atgagcttct gtaaaagtga ttgtatcact
     3721 ttaacatcag cattttttta gttagagtaa attcattagt aacaatttta tagaaataag
     3781 tgccgctgct aagactgtat gcattaaata caaccgagtg cctgccagca ggtttatatc
     3841 cgttaactaa tgtagcaact ttatttccaa gaatgttgta aactgatatt tcaacattac
     3901 ccggattttt taatccaaaa ttaattctgg tagatggatt aaaaggattg ggatagtttt
     3961 gtgctaaata ataattatca actaccgtgt tttcatcctc aacagatgtt ggatttacta
     4021 ttgcagtaaa gtcaactgta actctgctgc taggatttct aaaagttgcg gcttgcatct
     4081 gaacataacc cgttcctaaa ttaactaaag gaaaaacgtg tatcgcaact tctctggttt
     4141 cattaggtgc taaaggggta ctgccaaaat cagaattagt tgcaacactg tcaacccatc
     4201 ctggaaaaca atattcaaaa cataaagatg attgccagga agctggaaga ttattttcag
     4261 ttcttaccat aaatagggtt tggtccgcct gggtaatatt tgttacatca atgtaaaata
     4321 tcatttctga acccagtgta tcatatagaa ctgtttctct gggaacaaat gtaatgtcct
     4381 gagaaaatat tgaccccgta agcagaaaaa ccataaagaa acttagtaat tcttttatca
     4441 tataaactcg ttttaaatat taataaagag tggtgcactt agcaccactc caaatagata
     4501 acttatttta ttaatgtcat tttcttaatg tctgaataga ctgatccatc aattccagca
     4561 gcatccaatc tataaatata aacaccactg tttaaattct gtgctctaaa atcaacagag
     4621 tgatttccac ttgcaagatt accatttaat atggttacaa cttcttctcc aagaagatta
     4681 aataccttta atgtaacttt agattctgat gcaagattga attcgatcct cgttgaagga
     4741 ttgaaaggat ttggataatt ctgtttgagt gagaaatact gtggcccgat aacatctaca
     4801 tttacttctt cagaatattc aaatgatccg gttaaatcga cttgttttaa tctataagta
     4861 tatgaaccat tctgtaggtt attgtctgta taggaataat tctgaacttc agttgttgtt
     4921 cctttcccat taataaatcc aacagctaca aattctcccg ctactttttt ctgtatctca
     4981 aagccaagat tatttacttc tgttgccgtt gaccaattaa gatgaacgct acttccaacg
     5041 acttttgccg taaatgattt tagctcaacc ggtaatacag actcaagcga gtaagagaat
     5101 gttgcccact ggcttgctga tgatggaaca aaggcatttg tgcttgctgg tacagttgct
     5161 aatgcagctc cggatttatc aaaaatagta tgagggaatg ttaacccgcc atcaacagaa
     5221 agtttaacaa ctagtctatc tgtatatcca ggataattag catgtgcata attaaatttt
     5281 actgaatctg taacctcagc gccattaaat actttactta ttaaagtatc cgatgcaccg
     5341 gttgttgaat aactatagaa atccatttta acggatttgc ttcctgcgat agaaggtccg
     5401 ttagctccgg taaattggct aaatgtaatt cctccatccg gattttttag cgtccatcca
     5461 tttgccggaa aactgccttc aaataattca taattaaaaa cactattaat tccatcggtg
     5521 tttacaacaa cagatgccgg ttttgattgc acaacaaaat cagaagctga aactggtttt
     5581 tcaataatag tattttgatt atttacaatc atgttttcta aaacttcgtt tcttccctta
     5641 gcgctgttca gaacttcttt ggtttgatca ttctgcacaa acgcagcagt ataaatcatt
     5701 gaatcaaccc aaactgcttt atctatagca taagtaattg tataattata ggtgcctact
     5761 ccggtcggta cagaagttcc caaagagttt ggatatgctt ttctgaaaac atcataaaaa
     5821 tctttctcgc catttgttcc gggtgctgta gcatatttaa tatgtctctc tactgcatga
     5881 actctcaaat aataacttcc tggatttagt ggagataaaa cctgaacagt aatatcagct
     5941 cttacgctgt cgccgtttga aattcttgtg tctgttacag acatagctat tggagtacca
     6001 actttttttc ttggattgaa tgcacccggc aaacttgtgg gatcagaata tggatatgaa
     6061 ggatcgacaa caccatccat aattacatgc ggaacagcgt tgacgccata gtaactcctt
     6121 ctgtcagtag cttgtgtagg attgtataaa tacatcgggt cgttaccagg tgaaggccaa
     6181 ttcatatggt actttatcgg tacaattgaa tcaaatcgtg cagcaacaaa tgcatctata
     6241 gttggattat ttgccgcaca gggtccgcaa gttgagctgg tccattcttc taataaaata
     6301 tttctgaatg ttccaggcaa gtatattgta tactgattaa gtgtattatt tgttgagttt
     6361 tcatctccgg caagatttgt tacgacagaa atgtcgatcc cttgccccgg tgtaatggta
     6421 agatcatcaa aagtaacatt tgtggattga ccactgttta gagatgtaat tgtttttgta
     6481 cttgtatatc ctcccggggt agcggtcata gtaacattaa atgaggtagt aatattttgt
     6541 tttccaatat ttataactga tacattagga gtaataacaa acggtaaggt agatgtagca
     6601 taacttgtac ccgagttaat attattgata gaaccaactg ctgcatccaa attaaactga
     6661 ttcccgacag ttacattatc tatgtagaga ttgtttccgt aaccgttaat agtcacaaac
     6721 tttaattgta cctgtgcaaa agtaaaaaca cttagcgcag caaaccacga aaggaaaaac
     6781 aatgtggctt ttttcatatg aacttctcct ctgaaaatga atttgatagt taactagata
     6841 caaagaactt aattgcttaa aaactcgatc ccgtttgctg t
//
LOCUS       DASWBY010000454         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_131162, whole genome shotgun sequence.
ACCESSION   DASWBY010000454 DASWBY010000000
VERSION     DASWBY010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131162"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VF870_08105"
     CDS             complement(<1..206)
                     /locus_tag="VF870_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEX9252190.1"
                     /translation="MQRIKIDLPKKFIFSTEISVRVYDVNFAGHLSNDSILSMVHEAR
                     ILFLKNWGYSEVNTCGAGIIMFDAA"
     gene            complement(281..1573)
                     /locus_tag="VF870_08110"
     CDS             complement(281..1573)
                     /locus_tag="VF870_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9252191.1"
                     /translation="MVNNSTKLFEEIFSEEIISNESFRSKVLAGIIGFLIIVVLLISI
                     AFEKLFADLSHFPIMIELTLIILAVIFVRALFVSRAVKKWNRFGVKAFITIRYINTFI
                     EISIPSVVLIIYSFNLPSVYPLFTPIALMYFLIIMLSSLELDFKLCVFSGTVAAIQYL
                     IVVWYLTNKPIPDESIMSFSFLPSYVGTAVLFFISGHTAGLITNQIKKGLLKYYKAQS
                     ERNEIQKLFGQQISKEIVDELVKNEYRVQSRVRFVAIMFLDIRNFSFFAQNKSPEEII
                     AYQNIIFSFIIEIINKNKGIVNQIMGDGLMAIFGAPLENENDCQLAVNSALEIHTELK
                     RRNDEGLIPETVIRIGINAGEVVTGNVGTSERKQYSITGQPVIIAARLEQINKELGSA
                     ILISNTVYERVSLENEPINHKDITIKGIPDPITVYQLV"
     gene            complement(1624..>1825)
                     /locus_tag="VF870_08115"
     CDS             complement(1624..>1825)
                     /locus_tag="VF870_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9252192.1"
                     /translation="FAPIENMPHWARILAYMNPVSYFIDVMRMIVLKGSGFTDILRNL
                     GIISLFAIGFNILAILNYKKTV"
BASE COUNT          638 a          301 c          277 g          609 t
ORIGIN      
        1 gccgcatcga acataattat tcctgccccg caggtattaa cttctgagta gccccagttt
       61 ttaagaaata gtattcttgc ctcatgcacc attgaaagta tagaatcatt gctaagatgt
      121 ccagcaaagt ttacatcgta aactcttacg gaaatttccg tggagaaaat aaactttttt
      181 ggaagatcta ttttaattct ttgcatactt agtctttata aaaaatatat gccgtaaaat
      241 acagaaaaga aataaatctt tttaaaaagg aaagctttac ttacaccaat tgataaacgg
      301 ttataggatc cgggatgcct ttaatggtaa tatctttatg attaattggt tcattttcta
      361 agctaacacg ctcatagacg gtattggaga taagaattgc agaaccaagt tctttattta
      421 tttgttcaag cctggcggct ataattacag gctgaccggt aattgaatat tgtttccgct
      481 cactagttcc aacatttcct gtgacgactt cacctgcatt aataccaatt ctgataacag
      541 tttcagggat taatccttca tcatttcttc ttttaagttc ggtatgaatt tctaatgcag
      601 aatttacggc aagctggcaa tcattttcat tttcaagtgg agcaccgaag atggccatca
      661 aaccatcgcc cataatctgg tttacaatac ctttattttt gttgatgatt tctatgatga
      721 aagaaaaaat tatgttttga tatgcaataa tttcttcagg cgatttattt tgtgcaaaga
      781 atgaaaaatt tcttatatct aaaaacatta ttgcaacaaa ccttacacga ctttgaactc
      841 gatattcgtt tttaacaagt tcatcaacaa tctctttaga gatttgctga ccaaaaagtt
      901 tttgaatttc atttctttca gattgtgctt tataatattt caacaaacct ttttttattt
      961 gatttgttat aagtccagca gtatgtcctg aaataaaaaa tagtactgca gttcccacgt
     1021 aggaaggtaa gaatgaaaaa gacataattg attcatctgg aataggtttg ttagttaaat
     1081 accagaccac aattagatat tgaatcgcag ctactgttcc agaaaaaacg caaagtttaa
     1141 aatcaagctc taaggatgaa agcatgatga ttaaaaaata catcaatgca atgggagtaa
     1201 ataatggata taccgaagga agattaaagg aatatattat caatactacg cttgggatac
     1261 taatttcgat gaaggtatta atatatctga tagtaataaa agctttaaca ccaaatcgat
     1321 tccatttttt tacagcacgg cttacaaata aagcacgaac aaaaattact gcaagaataa
     1381 ttaaggtcag ctctatcatg attggaaaat gagataggtc tgcaaaaagt ttctcgaatg
     1441 caattgatat taaaaggact actataatta aaaacccaat tataccggct agcacttttg
     1501 atcgaaaact ttcattagag ataatctctt ccgaaaatat ttcttcaaaa agttttgtac
     1561 tgttattaac cattgaaatt aaatcattcc taattaaagt tttttttgaa acatattaat
     1621 ttactaaact gtttttttgt aattcaatat tgccagtata ttaaaaccaa ttgcaaataa
     1681 agatattatt ccaaggttac gtaaaatatc tgtaaaaccg cttcctttta aaactatcat
     1741 cctcattaca tcaatgaaat agcttaccgg attcatgtac gcaagtattc gagcccagtg
     1801 aggcatattt tcaattgggg caaac
//
LOCUS       DASWBY010000455         4574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_655926, whole genome shotgun sequence.
ACCESSION   DASWBY010000455 DASWBY010000000
VERSION     DASWBY010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655926"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VF870_08120"
     CDS             complement(<1..657)
                     /locus_tag="VF870_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252193.1"
                     /translation="MSDTIENNEITRLVKMLDSINARLSAIEATINLPKQADPDLIDQ
                     RVEPIQKETSEDLEFRLGEQWFGKIGIVAFLLAVFNFLVLPFASIPYHLILFSGFFIS
                     IGLIASSLFGAKLLKNLAGYTMGSGLILLFVSTLRLHFFSTDPILNNKMILIVLLFFV
                     TAVSLVLSLKKDSVYLTIWSLIFFFSAALISDFPILIFAALIFGGLGVSYLDKKNNWN
                     S"
     gene            complement(685..2145)
                     /locus_tag="VF870_08125"
     CDS             complement(685..2145)
                     /locus_tag="VF870_08125"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9252194.1"
                     /translation="MNYIPKLKLITIVLSIFIIQYTYAADDQCFSCHQGLEDKPSQLF
                     MEDVHYKAGLLCSSCHGGNSNSDDMDLAMNKSEGFIGVPVSNIISQICSKCHSDIDFM
                     KKYNSNIHTDQFTKLKESVHGKLAVNGKGMILQCTTCHNAHGIKKVNDPKSPVYPTNV
                     PQTCNKCHGNASFMQSYNPAISVDQLTKYRTSVHGVLNSRGNIKAAECASCHGSHNIY
                     PANDVRSKVYKLNIPGTCSQCHSDKNYMKAFNIPTDQFEKYKKSVHGIAVFEKQDLSA
                     PVCNDCHGNHGATPPGIESISKVCGSCHALNAELFSASPHKKAFDEKKYPECETCHGN
                     HEILIAKDQLLGVGKGAICLKCHSENENQKGYLIAKEMKKLTDSLIVSDSVAKNMVFQ
                     AEQKGMDVEELKFKLREIHQARLEGRTIVHSFDEAKFKEVATKGFKSSDVIITEAKAA
                     VDEFYFRRYGLGVAVAVITFLIVILFLYIKKIEKTK"
     gene            complement(2142..3224)
                     /locus_tag="VF870_08130"
     CDS             complement(2142..3224)
                     /locus_tag="VF870_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX9252195.1"
                     /translation="MEKKLKQIYEWVDSRVQLADLVKFMSKKYVPVHSRSIWYYFGGV
                     SLFFFIIQVVTGILLLFYYKGSSDLAYESIHFIMSKVQFGWLIRSIHSWSANLFILSV
                     FIHMFSVYFEKSYRNPREMTWISGVFMFVLALAFGFSGYLLPWNELAYFATKVGTDIV
                     GAVPVVGDPLKYFLRGGDDVTGATLSRFFGFHVALFPGIFTFLLIVHLLLVQRQGMSE
                     PLHFQNSAKEEKKTMPFFPNFMLRDLLLWLVVLNILAILAVFFPADLGLKADPLASAP
                     AGIQPEWYFLFMFQTLKFLPAHVFFIEGEVLGVLLFGLAGVLWLLVPVWDRKSARGQQ
                     NRLINYIGIFSVMFIIILTIIGWVLK"
     gene            complement(3231..3689)
                     /locus_tag="VF870_08135"
     CDS             complement(3231..3689)
                     /locus_tag="VF870_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX9252196.1"
                     /translation="MDINENNTAEKNNRRSFLKVLLTGGLAGFAAITIYPILSFLNPP
                     KQTEVEVTSVVVGKVDDIKPGDSKIIRFGNKPVIIIRRDENNFTALSATCTHLDCTVQ
                     YKKDEKIIWCACHNGKYDLYGKNVSGPPPRPLDKFNVAIKNNELIVSKEA"
     gene            complement(3721..4278)
                     /locus_tag="VF870_08140"
     CDS             complement(3721..4278)
                     /locus_tag="VF870_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX9252197.1"
                     /translation="MHNIFSFLLSIIITLGLSGSTANANGKSGDHKYVGTKLCAMCHK
                     SETAGKQFVIWEGSKHAQAFKTLTTAEADKIAKEKGFTTKASETEACLKCHATGYNVD
                     ASLLTDKFNISDGVQCETCHGPGSEYKTKKIMEDKKLAVENGLKIYDNIEELCVKCHN
                     EESPSFKGFNFKEMWAKIEHSVPKK"
     gene            complement(4308..>4574)
                     /locus_tag="VF870_08145"
     CDS             complement(4308..>4574)
                     /locus_tag="VF870_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252198.1"
                     /translation="ELQQDILDLIIFDSANQFADCLKLVKIIRKIKPKTPLIVISDDV
                     ENIEGGKIYEEGIFHLAQKPIDKIIIKEIIGASINTFKQNSKKS"
BASE COUNT         1480 a          858 c          698 g         1538 t
ORIGIN      
        1 agaattccaa ttgttctttt tatcaagata actaactccc agaccaccaa aaataagcgc
       61 tgcaaaaatt aaaattggaa aatctgaaat aagtgctgca gaaaagaaaa atattaatga
      121 ccagattgtt aaataaaccg agtctttttt tagcgacaat accagggaaa ctgccgttac
      181 aaaaaagagt aaaacaatta aaatcatttt attatttaag ataggatctg tgctgaagaa
      241 gtgaagtctt aaagttgata caaataacaa gatcaatccg ctgcccattg tataaccggc
      301 aagatttttt aacaactttg ctccaaagag cgaggaagca ataagtccaa tagaaataaa
      361 aaatccggaa aatagaatta agtgatatgg tatacttgcg aatggcagta caagaaaatt
      421 aaaaacggct aatagaaaag ctactatacc aattttacca aaccactgtt cgccaagtct
      481 gaattctaaa tcctcactgg tctctttctg aattggttca actcgttgat ctattaaatc
      541 aggatcggct tgtttaggca aattaatagt cgcttcgata gcagatagtc ttgcgtttat
      601 gctgtcgagc attttaacta atctggtaat ctcgttgttt tctattgtat cactcataat
      661 tcaccttaaa atattttagg acaattattt ggttttttct atttttttta tgtataaaaa
      721 aagtataacg attaaaaatg ttattacagc cacggcaaca cctaaaccat atcttctaaa
      781 ataaaactca tctacagctg ctttagcctc agttattatt acatccgaag atttgaatcc
      841 ctttgtggca acttctttaa attttgcttc atcaaaagaa tgtacaatcg ttcttccctc
      901 tagcctggcc tgatgaatct ctcttaattt aaacttaagt tcttccacat ccattccttt
      961 ttgttcagct tgaaaaacca tgttttttgc gactgaatct gaaacgatca gactatcagt
     1021 aagctttttc atttctttag ctattaagta tcctttttga ttttcatttt ctgaatgaca
     1081 ctttagacaa atagctccct taccaactcc caaaagctga tctttagcta taagaatttc
     1141 atgattgcca tggcaagttt cacattcagg atattttttt tcatcaaatg cttttttatg
     1201 cggacttgct gaaaacaatt ctgcatttaa tgcatgacaa ctgccgcata ctttagaaat
     1261 agattcaatt ccgggaggtg ttgctccatg attaccatgg caatcattac aaaccggtgc
     1321 agataaatcc tgcttctcaa aaactgctat gccatgtaca cttttcttat atttttcaaa
     1381 ttgatctgtg ggaatattaa aagccttcat ataattttta tcactatgac attgactgca
     1441 agtacccgga atattcaatt tgtaaacttt tgatctgaca tcattcgcgg ggtaaatatt
     1501 atggctgcca tggcagctag cacattcagc agcctttata tttcccctgg aattcaatac
     1561 accatgcacg cttgttctgt attttgttaa ttgatccacg gaaattgcag ggttataaga
     1621 ttgcataaac gatgcattac catgacactt attacaagtt tgaggaacat tggttggata
     1681 aaccggtgac tttggatcat ttactttttt tatgccatga gcattatgac aggttgtaca
     1741 ctgcagaatc attccttttc catttacagc caatttgcca tgaacacttt cttttagttt
     1801 tgtaaattga tctgtgtgaa tgttggaatt atactttttc ataaaatcaa tatctgaatg
     1861 gcactttgag catatctgtg atataatatt cgataccggt actccaataa atccttccga
     1921 cttattcata gccaggtcca tatcatcact attggaattt ccaccatgac aactactgca
     1981 taataatcct gctttatagt gtacgtcttc cataaataat tgtgagggtt tatcctccag
     2041 tccttgatga caactaaaac attgatcatc agctgcataa gtatactgga taataaatat
     2101 tgataagaca atcgttatta atttaagttt aggtatataa ttcattttaa tacccatccg
     2161 ataatggtaa gaataattat gaacatgaca gaaaaaattc caatataatt aattaaacga
     2221 ttttgctgac cccttgcact ttttctatcc cacactggaa ctagtaacca taacacacca
     2281 gcaagtccaa atagcaatac acccaatact tcaccttcaa taaagaaaac atgagcgggt
     2341 aaaaatttta aggtttgaaa cataaacagg aaataccatt ccggttgaat tcctgctggt
     2401 gcagaagcta gaggatccgc ttttaatcca agatcagccg ggaaaaacac agcaagaatt
     2461 gcgagaatat ttagaacaac aagccaaagc aaaagatctc ttaacataaa attaggaaag
     2521 aacggcattg tttttttctc ttctttagct gagttctgaa agtgcagagg ttcactcata
     2581 ccttgccttt ggactaaaag caaatgaaca atcagcaaaa aagtaaatat gcctggaaac
     2641 aatgctacgt gaaaaccaaa aaatctggaa agagtagcgc ctgttacatc atccccgcct
     2701 ctaagaaaat atttaagagg atcacctaca acaggaactg cacctactat atctgttcca
     2761 acttttgtag caaaatatgc cagttcattc catggaagca gataaccgct aaatccaaat
     2821 gctaaagcaa gaacaaacat aaatacaccg ctaatccatg tcatttcgcg tggatttctg
     2881 tacgattttt caaagtatac gctgaacata tgtatgaata cactcagaat aaaaaggttt
     2941 gcagaccagc tatgtattga tcttatcagc catccaaatt gaaccttaga catgataaaa
     3001 tgaatacttt catacgcaag atctgaactt cctttataat agaacagcaa cagaattccg
     3061 gttacaacct gaattatgaa gaagaataaa gagacgcctc caaagtaata ccatattgaa
     3121 cggctatgaa caggaacgta tttcttactc ataaatttaa caagatcggc tagctgtact
     3181 cggctatcca cccactcgta tatttgtttt aatttttttt ccataattaa ttatgcttct
     3241 tttgaaacta ttaattcatt attctttatt gcaacattaa atttgtccaa tggtcttggt
     3301 ggagggcctg aaacattttt accgtataaa tcatacttac cattgtgaca ggcacaccag
     3361 ataatttttt catctttttt atactgaaca gtacaatcaa gatgagtgca tgttgcagat
     3421 agagctgtaa aattattttc atcccttcga atgatgatta caggtttatt tccaaaccta
     3481 attatttttg aatctcctgg tttaatgtca tcaacttttc caacaactac actagttact
     3541 tctacttccg tttgcttggg aggatttaaa aaggaaagaa ttggatagat cgttatagca
     3601 gcaaatcccg ctaaccctcc tgtaagcaaa acttttaaga aacttcttct gttatttttc
     3661 tctgctgtat tattttcatt tatatccatt tagcatttcc aatttgaata ttaaaatcat
     3721 ttatttttta ggaactgaat gttcaatctt ggcccacatc tctttaaaat taaatccttt
     3781 gaaagaagga ctttcttcat tatggcactt tacacaaagt tcttcgatgt tatcataaat
     3841 ttttaatcca ttctcaactg caagtttttt gtcttccatt atcttctttg ttttatattc
     3901 agaaccaggt ccgtgacaag tttcgcattg aacgccatcg gagatattga acttatctgt
     3961 taacaaagat gcatcaacat tgtaaccggt tgcatgacat tttaaacagg cttccgtttc
     4021 agaagcttta gttgtaaagc ctttttcttt tgcgatctta tcagcttctg cagtagttaa
     4081 agttttaaaa gcctgtgcgt gtttacttcc ttcccagatt acaaattgtt ttccggcagt
     4141 ttctgattta tgacacattg cgcataattt tgtgcctaca tacttatgat ctccgctttt
     4201 accatttgca ttagcagttg acccggatag acctaatgtt attattattg ataatagaaa
     4261 tgagaatatg ttgtgcatgt tttaactcct gtataagtga agatagttta tgatttttta
     4321 ctgttctgtt taaaagtatt gatcgaagca cctataattt ctttaataat aattttatcg
     4381 attggttttt gagcgaggtg gaaaatgccc tcttcataaa tttttccgcc ttcaatgttt
     4441 tctacatcgt ctgaaattac gataagagga gttttcggtt taatctttct aataatcttt
     4501 acaagtttta agcaatcggc aaattggtta gcggaatcaa aaataatcag atcaagaata
     4561 tcctgctgaa gttc
//
LOCUS       DASWBY010000456         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_54135, whole genome shotgun sequence.
ACCESSION   DASWBY010000456 DASWBY010000000
VERSION     DASWBY010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54135"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VF870_08150"
     CDS             complement(<1..552)
                     /locus_tag="VF870_08150"
                     /inference="COORDINATES: protein motif:HMM:NF017765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HEX9252199.1"
                     /translation="MDFQILISNITNPTLLFFLLGIIAVTIKSDLQIPESSSKFISLY
                     LLFAIGFKGGQELAHSEYSAEITYTLLFGVAMASVVPAYTFFILKRKLSSSYSGAIAA
                     AYGSVSAVTFVAAVSFLEMQQISYGGHMVAVMALMESPAIVIGVIMMMMFGNESAKEV
                     NWKSIIQHSFTNGSVVMILGSLII"
     gene            complement(811..1263)
                     /locus_tag="VF870_08155"
     CDS             complement(811..1263)
                     /locus_tag="VF870_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252200.1"
                     /translation="MRLNLEELHHRIRTDNKFKKRLRKIVLIGGIGILLIAVLGVVGL
                     VFFSSAIISFLFSNAPTVYELAFNYVRDFAGSFMLEDITAMLDPLTGGTNVNEMKNLI
                     TQYFNQLSSNPDIDFQNFQNFITTIKSSLLDNQISNTELDSVRQFLLN"
     gene            complement(1283..2146)
                     /locus_tag="VF870_08160"
     CDS             complement(1283..2146)
                     /locus_tag="VF870_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9252201.1"
                     /translation="MKDKVVIITGSNKGIGKEAAKEIAKLGAKVYMACRTLDSANQAR
                     EEIVNETGNQNVFVKHLDLASPDSILNFVDLFKKEESKLDVLINNAGLWTKTKKLTDI
                     NVEQTFAVNVLGHQLLTQLLLNELKNAAPSRIINTASHFAGGLDIDDINFNKRNYNET
                     LAYKQTKQANRMLTRKWARRLEKDNISVYSLTPGFIPDTELFREQNIVGKFLLKSFAL
                     IEGRTIQEGADTIVWLASAEKIDGSNGGFFNQRKEEKCKFNNPADEKKLWDKCEEFLS
                     KIKVNDSVFQS"
     gene            2163..2495
                     /locus_tag="VF870_08165"
     CDS             2163..2495
                     /locus_tag="VF870_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HEX9252202.1"
                     /translation="MADVSLYNNKSGKFVPRLFERKGKVKCLFAMKSFNKEDISRIIE
                     MAWEDRTPFEVIELQFSLKENEVREIMRSELKPSSFKMWRKRVSGKKTKHLSLRSKEV
                     KRFKSKNQ"
     gene            complement(2549..2710)
                     /locus_tag="VF870_08170"
     CDS             complement(2549..2710)
                     /locus_tag="VF870_08170"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEX9252203.1"
                     /translation="MASKLGIDSNALASQLSNLLSDVVDKLTPDGKVPEGDIMSQVSD
                     LRGGLFGKK"
BASE COUNT          880 a          478 c          448 g          908 t
ORIGIN      
        1 aattatcaaa cttcccaaaa tcataactac acttccattg gtaaaagaat gctgaattat
       61 ggatttccaa ttcacttctt ttgctgattc atttccgaac atcatcatca taataacacc
      121 gattactatt gccggtgatt ccattaaagc cattacagca accatgtgtc cgccgtaaga
      181 aatttgctgc atctccagaa aagaaactgc ggcaacaaaa gtaacagcac ttacagaacc
      241 atatgctgca gcaatcgctc ctgagtaact tgagctgagt tttcttttaa gaataaaaaa
      301 agtatacgct ggaacaactg aagccatagc aacaccaaac aaaagagtat aagttatttc
      361 agcactatat tcactatgag ctaactcctg tccgccctta aaaccaattg caaataaaag
      421 atatagtgaa ataaatttgc ttgatgattc cgggatttgc agatcacttt taatagttac
      481 ggcaataatt cccagaagaa aaaaaaggag agtaggattg gtaatgttag atataagtat
      541 ctgaaaatcc atagtgtttt gttgaaattt tagtgttgtg ttgtgctaat gtttataaat
      601 tctaaaagct attgggttta aaatatgaaa agtttgcttg ttttaagaga tggtgctgtg
      661 gaaaattttc tggtagctta gttggtacaa tgctttactg atgtaatgag tttaagattc
      721 tgattaataa ttttttaaat gagttaagca gaagcagaga taccttacat agagtgttct
      781 ccgcttccga ttattccgac tttcatattc ttaattcaat aaaaattgtc ttactgaatc
      841 cagctctgta ttacttattt gattatccag caacgaactt tttatcgttg taataaaatt
      901 ctgaaagttt tgaaaatcaa tatctggatt actgctaagc tgattaaaat actgtgtaat
      961 aagatttttc atctcattta catttgtacc gccggttagc gggtccagca tcgcggtgat
     1021 atcctcaagc ataaacgagc cagcaaaatc acgcacataa ttaaaggcta attcgtaaac
     1081 agtcggtgca ttagagaata agaagctaat tattgcagaa gagaaaaata caagtcctac
     1141 gacacccaga acagcaataa gcaaaatacc tattccgcca atcaggacaa tttttctcaa
     1201 acgtttttta aatttattat ctgttctgat cctatgatgg agctcttcta aattgagtct
     1261 cataaatttt atccaaattc tattatgatt gaaaaactga atcgttaact tttatcttgc
     1321 tgagaaattc ctcacattta tcccatagct tcttttcatc agcaggatta ttgaatttac
     1381 atttctcttc ttttctttga ttgaagaatc ctccgttgct tccatcaatt ttttcagcgg
     1441 atgcaagcca aacgattgta tctgcacctt cctgaattgt tcttccttca attaatgcaa
     1501 aagattttaa taagaatttt cccacaatat tctgctctcg gaaaagttcg gtatctggaa
     1561 tgaatcccgg ggtcaatgaa taaacagaaa tattatcttt ctctaatctt cttgcccatt
     1621 tacgcgttaa cattctgttt gcctgtttgg tttgtttgta agcaagagtc tcgttgtaat
     1681 ttcttttatt aaaattgatg tcatcaatat caagtccgcc cgcaaagtga gaggcagtat
     1741 ttataattct tgacggagct gcatttttca attcatttag aagcaattgt gttaaaagtt
     1801 gatgaccaag aacattaacg gcaaaagtct gttcaacatt tatatcagtt aacttttttg
     1861 tttttgtcca gagtccggcg ttgtttatca gcacatcaag ttttgattct tcttttttga
     1921 acagatcgac gaaatttaat attgaatctg gggaagcaag atcaagatgt tttacaaaaa
     1981 cattttgatt tccggtttca ttaacaattt cttctcttgc ctgatttgca gaatcaagtg
     2041 ttctgcaagc catataaact ttagctccaa gctttgcaat ttcttttgca gcttcttttc
     2101 cgattccttt atttgagcca gttattatta ctactttatc tttcatttta tcctcttttt
     2161 cgttggctga cgtttcatta tataacaata aaagtggaaa gttcgttcca aggttatttg
     2221 agcgaaaagg caaggtcaaa tgtttatttg ctatgaaaag ttttaataaa gaagatatct
     2281 ccagaataat agaaatggct tgggaagacc gcaccccgtt tgaagtgatt gaacttcagt
     2341 ttagtttgaa agagaacgaa gtgcgagaaa ttatgagaag cgaattgaaa ccctcaagtt
     2401 ttaaaatgtg gcgtaaaaga gttagcggaa aaaagacaaa acatctttct cttcgttcaa
     2461 aagaagtgaa aaggtttaag agtaagaatc aataaaaaag ccgcagcatt acagttacgg
     2521 cttaaaaatt acaaatttta aaacttattt acttcttccc aaacaatccg ccccgcagat
     2581 cagagacctg actcataata tcaccttcag gaacttttcc atctggtgtt aatttatcaa
     2641 caacatcaga aagaagattt gaaagttgag aagctaacgc attgctatct attccaagtt
     2701 tagatgcgat attt
//
LOCUS       DASWBY010000457         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_354449, whole genome shotgun sequence.
ACCESSION   DASWBY010000457 DASWBY010000000
VERSION     DASWBY010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354449"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..2041
                     /gene="dxs"
                     /locus_tag="VF870_08175"
     CDS             119..2041
                     /gene="dxs"
                     /locus_tag="VF870_08175"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX9252204.1"
                     /translation="MSDTVKYPVLSKVNYPSDIKQMDITELKVLCKDIREYLVDTISE
                     IGGHFGGGLGTVELTVAIHKVFNTPYDLVVWDTGHQAYPHKILTGRKEALKKIRRLNG
                     ISGFLKRSESEYDAFGAGHATTSISAALGMAAARDLNNEQNRKVIAVIGDGAMTGGMA
                     YEAMNNSGVLKSDIIVVLNDNNMSIAPNVWQISNYFTEMISHPEYNKFKGQVWDLTGK
                     LDHFGDRLRKIAARLESGIKAVVTPGMLFEALGFRYFGPVNGHNLHQLIKIFEQTKQF
                     KGPILIHALTEKGKGYKPAENHVQRLHASTPFDKVTGEAHKKSGAAASYTKIFGEALV
                     EIVKSNSKVIGITGAMPGGTGLDYLQKACPKNYFDVGIAEEHGVTFAAGMATQGIIPV
                     VAMYSTFLQRGFDQIIHDIALQKLHVVFVMDRAGLVGADGPTHHGVFDISYLRMIPGM
                     VIMAPKDEAELRDMLYTAVECKDGPIALRYPRGSALGVEVKEGFSKIEIGKAEKLVDG
                     EDVALLALGSMVDYSLKAAAKLKEQGISCEVINMRFAKPLDTEMLDSVAGRFNKIITL
                     EENNLPGGFGSAVAEYFILKNYKNDLQFIGIPDKFVDHGTQEELHHILGIDPDGIAEK
                     VKQFIQLNKINHKVSI"
     gene            2041..3042
                     /locus_tag="VF870_08180"
     CDS             2041..3042
                     /locus_tag="VF870_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9252205.1"
                     /translation="MEKVKIGVIGLGGVAQLVHLPNFAKIPNAELTAVAETNKNRLLT
                     ISDKFNVKQRYSNYKDMLEKSDIQAVIIATPTSTHKDIAIDCFKAGKDVLIEKPMART
                     YQEAKQIFEAAKKSKKILMVGMNLRYRPDTMLLRSFINSNEIGEPFYVKCGWIRKQSS
                     SEKWFTKKEESGGGVITDLGIHIMDLALWLLGYPEISSVSAQNFHHNTKNVEDTSISC
                     IKCNNKAVINMEVSWSLPVEKNHFFLDVFGTKGSFSSNPFRLYKKVENDYINLTPTQV
                     DTPTVLLKKSYLNELKSFIGAIIGLNPNFSTGEEAMQRMKIIEAMYESAEKRQEIKL"
     gene            3047..3736
                     /locus_tag="VF870_08185"
     CDS             3047..3736
                     /locus_tag="VF870_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9252206.1"
                     /translation="MKKILLVDDEPDIVEFLKYNLEQQNFEVVVGYNGEEALQKLIDK
                     PDLIVLDIMMPKFDGFETCKRIRENKEFVNVPIIFLTAKAGELDEIKGLELGASDYIQ
                     KPISPNKLIARVKSNLRKAENNTSKNPEPIKIKYGPIDIDKEKYEVFIDGEKKVFPRK
                     EFEVLYFLVNNPGKVFGRDILLKEVWGEDVYVVDRTVDVHIRKIREKLENHAELIETV
                     KGVGYRLKSVE"
BASE COUNT         1321 a          547 c          804 g         1100 t
ORIGIN      
        1 aagaatgttt atccgttctt gaaaaagctg agcttaaagt aactacgcta aaaaaagaat
       61 taaacgaaat taataattta gaagaagatt aaaccctgat taaaaatgga gacgattaat
      121 gtctgacact gtaaaatatc ctgtactttc aaaagttaac tatccatcgg atataaagca
      181 gatggatata acggaattga aagtactatg taaggatatt cgtgaatatt tggtagatac
      241 gatatccgaa atcggtggtc actttggcgg cgggttagga actgttgagc ttactgttgc
      301 aattcacaaa gtgtttaaca ctccttatga tcttgttgtt tgggataccg ggcatcaggc
      361 atatcctcat aaaatattga ccggacgaaa agaggcatta aagaaaatta gaagactgaa
      421 tggcataagc ggatttttga aacgaagtga aagtgaatat gatgcttttg gtgccggtca
      481 tgcaactact tctatttctg cagcacttgg tatggccgcc gcacgcgatc tgaataatga
      541 acagaatagg aaagtaattg ctgttattgg cgatggcgca atgaccggtg gaatggctta
      601 cgaagcaatg aataattcgg gtgttcttaa atcagatata atagttgttt taaatgataa
      661 caacatgtca atcgcaccaa atgtctggca gatttctaac tactttacag aaatgatttc
      721 tcatcctgaa tacaataaat ttaaaggaca ggtttgggat cttaccggaa aacttgatca
      781 ctttggagat agattaagaa aaattgctgc acgacttgaa agcggaatta aagctgttgt
      841 aactcccgga atgttattcg aagcattagg attcagatat tttggtcctg tcaacggaca
      901 taatcttcat caattaataa aaatctttga acagacaaaa caatttaaag gacctatcct
      961 gattcacgca cttacggaaa aaggcaaagg gtataaaccg gctgaaaatc atgttcaaag
     1021 attgcatgcc tctacacctt ttgataaggt tacgggtgaa gcacataaaa aatccggtgc
     1081 agcagcatct tatacaaaaa tatttggcga ggcactggtt gagattgtta aaagtaattc
     1141 aaaggtaatt ggaattacag gtgcaatgcc gggtggaacc ggtttggatt atctgcagaa
     1201 ggcctgccct aaaaattatt ttgatgttgg aattgctgaa gaacacggag ttacgtttgc
     1261 agctggtatg gcgacacaag gtataattcc tgttgtagca atgtattcta ctttcctgca
     1321 aagagggttt gatcagatta tacatgatat cgctttgcag aaattacacg tagtttttgt
     1381 aatggataga gcaggattag tgggtgctga tggaccaacg caccacggag tttttgatat
     1441 atcatattta aggatgattc ctggaatggt tattatggca ccgaaggatg aagcagaatt
     1501 acgtgatatg ctttatactg cagttgaatg taaagatgga ccaattgctt tgagatatcc
     1561 aaggggatcg gcactgggtg ttgaagttaa agagggcttt agcaaaattg aaatcggcaa
     1621 agcagaaaaa ttagttgatg gtgaagatgt tgcattactt gcactcggat caatggttga
     1681 ttactcttta aaagctgctg caaaacttaa agagcaggga atcagttgtg aagtaattaa
     1741 tatgcgattc gcaaaaccgc ttgatactga aatgttagac tctgttgctg gcagatttaa
     1801 taaaataatt acgctggaag agaataattt accaggcgga tttggaagtg ctgtagctga
     1861 atatttcatc ttaaagaatt ataaaaatga tctgcagttt attggtatac cggataaatt
     1921 tgttgatcac ggcacacagg aagagcttca tcatatttta ggaattgatc cagacgggat
     1981 tgcagaaaaa gtaaaacaat tcattcagtt aaataaaata aatcataagg tttctatctg
     2041 atggaaaaag taaaaatcgg agtaattgga ttgggtggag tagcccagtt agtgcatctg
     2101 ccaaattttg ctaaaatacc aaatgctgaa ttaaccgctg tggcagaaac aaataaaaac
     2161 agacttttaa caatatcaga taagtttaat gttaagcaga gatattccaa ttataaagat
     2221 atgcttgaga agagtgacat tcaggccgtt atcattgcaa ctcctacaag cacgcataaa
     2281 gatattgcga ttgattgttt caaagcaggt aaagatgttc tgatcgaaaa acctatggct
     2341 agaacctatc aggaagccaa gcaaatattt gaagctgcaa aaaaaagtaa aaaaatattg
     2401 atggtaggaa tgaatctacg ttacagacct gatacaatgc ttttacgaag ttttattaat
     2461 tcaaatgaaa tcggtgagcc attttacgtt aaatgcggct ggataagaaa acaaagcagc
     2521 agcgaaaagt ggtttacgaa aaaagaagaa tccggcggcg gcgttattac tgatcttgga
     2581 attcacatta tggatttagc tttgtggctg cttggttatc ctgagatttc ttcagtatcc
     2641 gcacaaaatt ttcatcataa cacaaaaaat gtagaagata cttcaatatc gtgtataaaa
     2701 tgcaataaca aagctgttat taatatggaa gtaagctggt cacttcctgt tgaaaagaat
     2761 catttctttc ttgatgtgtt cgggacaaaa ggcagttttt catcaaatcc ttttagattg
     2821 tacaaaaagg ttgaaaatga ttacattaat ttaacaccaa cgcaggttga tactccaact
     2881 gttttgctaa aaaaatctta tcttaacgaa cttaaaagtt tcataggtgc aataattgga
     2941 ttgaatccta atttttctac tggagaagaa gctatgcagc gaatgaaaat aattgaagca
     3001 atgtatgagt ctgctgaaaa gaggcaggaa ataaaattat agcgaaatga aaaaaattct
     3061 tttagttgat gatgaaccgg atatagttga atttttaaag tacaatcttg aacagcaaaa
     3121 ttttgaagta gttgtcggat acaatgggga agaggctctt cagaagctta ttgataaacc
     3181 ggatttgatt gtacttgata ttatgatgcc taagtttgat ggatttgaaa cctgcaaacg
     3241 tataagagaa aataaagaat ttgtaaatgt gccgataatt tttcttaccg ctaaagccgg
     3301 agaattggat gagataaaag gtctggaatt gggcgcgagt gattacattc aaaaaccaat
     3361 ttcgccaaat aaattaattg cgcgggttaa atccaatctt agaaaagcag aaaacaatac
     3421 ttccaaaaat cccgaaccca taaaaattaa gtatggacct atcgatatag ataaagagaa
     3481 gtatgaagta tttatagatg gtgaaaaaaa agtttttccc cgaaaagaat ttgaagtgct
     3541 gtattttctt gttaataatc cgggtaaggt ttttggccgg gatattttat taaaggaagt
     3601 ttggggtgaa gatgtttatg ttgttgatag aactgttgat gttcacatca gaaaaatccg
     3661 ggaaaagtta gagaatcacg ctgagttaat tgaaaccgtt aaaggcgtag ggtacagatt
     3721 aaaaagtgtg gaatgaaatc aaatccaacg tctcttctgc gttagtttat tt
//
LOCUS       DASWBY010000458         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_131302, whole genome shotgun sequence.
ACCESSION   DASWBY010000458 DASWBY010000000
VERSION     DASWBY010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131302"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1815)
                     /locus_tag="VF870_08190"
     CDS             complement(<1..1815)
                     /locus_tag="VF870_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9252207.1"
                     /translation="MKKLKTKILLGLVFLLTVILLLSLTGIISIYYLSRDSDAIIRDN
                     YKSVEYSTNMLDALENIFNNQIILMVSETRDSNEIDYLKETIVQKKEIFTKYLELQKS
                     NITEVHEDSLVKTIIDSYARFNSLIDSSSLTNNYDDYLMNELKARYWIASASIRGIYT
                     LNMSAIYRKNSTANNTANNVTLYMTVAAISSIVLTLIFIFYFPSYVTNPISELTKKIE
                     DISNRKYDQRIDIQSNDELKTLAAAFNKMALSLKAYEAQHIDELLIEKRRMETLVTNL
                     QDGTLLLDKDFNVLLANDKFCSLTGLKKSELLGQNINEIQTDNWILEQFHLTGLKNIQ
                     ALIKENIKPISIKVDDEIEYYQILFLDIRKQFKTELLSESAGYILLLKNITKYEKRDL
                     AKTNLIATISHELKTPLSSINLSIKLLEDNRIGDLNKEQLELLGSIKLHSSRILNLVN
                     EVLDFAQVETGYIRLKIKSANITDIVELSTFAVLMMLNEKEINLELMISENLSKVKCD
                     LEKTVWIVVNLLSNAIRYSSKKGTISIEATQEKQFIRISVKDNGPGISVQEQNRIFEK
                     YVKSKTEPAKGTGLGLAIAKEFVEVQGGIIGVKSKPGEG"
     gene            complement(1812..>2527)
                     /locus_tag="VF870_08195"
     CDS             complement(1812..>2527)
                     /locus_tag="VF870_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9252208.1"
                     /translation="MFGYKAGAFTGAVKNKKGLFEEANRGTLFLDEIGDMDISLQAKL
                     LRVLENNTFIKQGDTKETSVDVRIIAATNKDLKKLINEEKFRIDLYYRISVIQINLPP
                     LRKRLEDIPIITDNFIKIFCEKLKKPAPQIHESFYTRISKYHFPGNTRELKNVIERII
                     LLNDKPILTEDDLPKDFFDSKEGAAFSNEMSLVDLEKKHILSVLKSTNNNKVEAAEVL
                     GIGLTTLYRKLNEYGFQDE"
BASE COUNT          765 a          426 c          354 g          982 t
ORIGIN      
        1 tccttctccg ggtttgcttt taacaccgat tataccgcct tgcacttcaa caaattcttt
       61 ggcgattgct aatccaagtc cggttccctt tgcgggttca gtttttgatt taacatattt
      121 ttcaaatatt ctgttttgtt cctgcacaga tatacccggt ccattatctt ttacagaaat
      181 acgtataaat tgtttttctt gtgttgcctc gattgaaatc gtcccttttt ttgatgaata
      241 tcttatggcg ttacttaata aatttacaac tatccagaca gtcttttcga gatcgcattt
      301 tactttagaa agattttcag aaatcattag ttcaagatta atttcttttt catttaacat
      361 cataagaaca gcaaaggtac tgagctcgac aatatcagta atgtttgcag atttgatttt
      421 tagtcttatg taccctgttt caacctgagc gaaatccaaa acttcattta ccagattcaa
      481 tattctgctg ctgtgtaatt taattgatcc caaaagctct aattgctcct tatttaaatc
      541 acctattcga ttatcctcta aaagctttat agaaagattt attgatgaaa gaggtgtctt
      601 taactcgtga gaaatagttg cgattaaatt agtttttgct aaatcacgtt tctcatactt
      661 tgtaatattc ttaaggagga gaatatatcc tgctgattca ctcaaaagct cagttttaaa
      721 ttgtttccta atatccagaa ataatatctg atagtactca atctcgtcgt caacttttat
      781 gctaataggt tttatattct cttttatcaa agcttgaata ttttttaagc cagttaaatg
      841 aaactgttcc aatatccagt tatccgtttg aatttcatta atattttgac ccaacaattc
      901 agatttcttt agacctgtta gactacaaaa cttatcattt gcaagaagta cgttaaaatc
      961 tttatcaagc agtaaggtcc catcttgtaa atttgttact aaagtttcca ttcgtcgttt
     1021 ttctatcagt aattcatcta tatgttgcgc ttcataggct tttaaactta gtgccatttt
     1081 gttaaaagct gcagccagtg tcttcagttc atcgtttgat tgaatatcta ttctttggtc
     1141 atattttcta ttggaaatat cttcaatttt ttttgttaac tcagaaattg gattggttac
     1201 atatgatggg aaataaaata taaaaattaa tgtgagaaca atacttgaaa ttgcagcaac
     1261 tgtcatatag agtgtaacgt tgtttgctgt attgttagcc gtactatttt ttctgtaaat
     1321 agcagacata tttagagtat aaatcccccg aatagatgcg gaagcgatcc agtatctagc
     1381 tttgagctca ttcatcaaat agtcatcata attattcgtt aaacttgatg agtctattaa
     1441 tgaattaaat ctggcgtatg aatcaattat cgtttttaca agactgtctt cgtgtacttc
     1501 cgtaatattt gatttctgta attcaagata ttttgtaaat atttcttttt tttgaactat
     1561 agtttctttc aaataatcaa tttcatttga atcccgtgtt tcactaacca tcagtataat
     1621 ctggttatta aaaatatttt ccaaagcatc aagcatgttt gttgagtact caacagactt
     1681 ataattgtct cgtattattg catccgaatc tctggaaaga taataaattg aaataatgcc
     1741 ggtaagactc aaaagtaata ttacggtaag aagaaaaaca aggcctaata atattttagt
     1801 tttaagtttt ttcattcatc ttgaaatcca tattcgttta actttctata caaagttgtt
     1861 aaaccgatac ctaaaacttc ggcagcttcc accttattat tgtttgttga ttttaggact
     1921 gaaagaatat gttttttttc taaatccact aaactcattt cattgctaaa ggctgctcct
     1981 tcttttgaat caaaaaaatc ttttggcaaa tcatcttctg taagaattgg tttatcatta
     2041 agcagaataa ttctttcaat tacattcttt aactcacgtg tatttcccgg gaaatgatat
     2101 tttgagattc gggtataaaa tgattcgtgg atttgaggag caggtttctt aagtttttca
     2161 caaaatattt ttataaaatt atccgtaatt attggaatgt cttccaatct ttttctaagg
     2221 ggtggtaaat ttatttgaat gacacttatt ctataatata aatcaattct aaatttttct
     2281 tcattaataa gttttttcaa atctttattc gtggccgcaa taattcttac atcaacagaa
     2341 gtctctttag tatcaccttg ttttataaaa gtattatttt ccagtacgcg caataatttt
     2401 gcctgaagtg aaatatccat atccccgatt tcatcaagga ataaagtccc tctattagct
     2461 tcttcgaaca atcctttttt atttttaaca gcacctgtaa aagccccggc tttgtatcca
     2521 aacatct
//
LOCUS       DASWBY010000459         4857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_54226, whole genome shotgun sequence.
ACCESSION   DASWBY010000459 DASWBY010000000
VERSION     DASWBY010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54226"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /gene="meaB"
                     /locus_tag="VF870_08200"
     CDS             complement(<1..740)
                     /gene="meaB"
                     /locus_tag="VF870_08200"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9252209.1"
                     /translation="MKKQSSNKNTSYKPDWTPENAGDEFAVRVVKGVKDLSSNKAGSN
                     LKRKNLSADDYVNGVVSFDRNILARTITLIESNNPSHHNLAQEVLKKLLPHSGNSLRI
                     GITGVPGAGKSTLIEALGMYLIKQGHKVAVLTVDPSSTVTKGSILGDKTRMENLSKEK
                     NCFIRPSPSGGNLGGVTRKSRETITVCEAAGFDIILIETVGVGQSEVTVRSMVDFFLL
                     VLIAGAGDELQGIKRGIIELTDAILINKA"
     gene            987..1397
                     /locus_tag="VF870_08205"
     CDS             987..1397
                     /locus_tag="VF870_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX9252210.1"
                     /translation="MLKVFLVLVGISLFVNACSTAGSITKLDDTEWSVTSLLGKTLKA
                     DDMMKGLPSLNFSENGKLFGSTGCNNFTGSYKLDGTKLSIDPGAMTKMFCPGDTEQDF
                     LKAISRITNYKLEGNTLNLLDGTNAVMKLAPKSK"
     gene            1681..3252
                     /locus_tag="VF870_08210"
     CDS             1681..3252
                     /locus_tag="VF870_08210"
                     /inference="COORDINATES: protein motif:HMM:NF025499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HEX9252211.1"
                     /translation="MNTIEQELKKAISYIEQNKSTEISISSIEEQLKLFYSGVHIPAL
                     AKPCMVGDGIHGIKDLEIDEILALYNEAANAGRLIKFVPASGVASRMFHKLQSVLIRF
                     NDFTLDDLKKKSEDEKECKSVLEILINLKKFAFYNELKSILNVDEAGLMKIIDETPAE
                     ILKAILFDNGLNYSSKPKGAIKFHRYENESRTAFEEQIYEALHYISDKEGNVKIHFTI
                     SEEHTNLFNTIIDQLKSNISNQYKVDVTYSFQKKSTYTIAVDIENKLIFDKSRQLVRR
                     PGGHGALLENLNNLKADVVIIKNIDNLSKENLASDTILYKKLLIGYLIKVQKQVFEML
                     YALNEKDFDQKRFDEMLDFAENDLFIIKPTGFENWSDEQKQKFLFSKLNRPIRVCGMV
                     KNEGEPGGGPFWVNDNNGSLSLQIVEHAQINKDDENQQNIFEQSTHFNPVDLVCGLRD
                     YKGRNFDLHNFVDHKSGIITKKSKDGIELKALELPGLWNGSMADWITIFVEVPISTFN
                     PVKELNDLLRKEHQN"
     gene            3300..3530
                     /locus_tag="VF870_08215"
     CDS             3300..3530
                     /locus_tag="VF870_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252212.1"
                     /translation="MKKKSFRSQLFRKEIQVNDPEYWIKVYAELLFRIDELEMQKCNC
                     FKCQTDLDRYLNFFQKLRDDKTYLYRSKLDSA"
     gene            complement(3819..4484)
                     /gene="rpiA"
                     /locus_tag="VF870_08220"
     CDS             complement(3819..4484)
                     /gene="rpiA"
                     /locus_tag="VF870_08220"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX9252213.1"
                     /translation="MNEKKLAAEKSIEFIKDGMTLGLGTGSTVFFLVNKLAELVKQGL
                     EVKCVSTSNQTSELAKSLGIKIVNLNEVDFIDLTIDGADEVDKNLNGIKGGGGALLFE
                     KIVAASSKKVIWIVDSSKYVEKLGKFPLPVEVIPFGSNHIIKRFMERGYNPVRRKNGN
                     NDYFTDSGNEIIDIHLNEIEDSIKLEKDIKLIPGIVEVGLFNNIADRVIIGRGNTTEI
                     ISR"
     gene            4595..>4857
                     /locus_tag="VF870_08225"
     CDS             4595..>4857
                     /locus_tag="VF870_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX9252214.1"
                     /translation="MHHYDPSIEIQDYLVFGEYGDVNPSITDSSTYTFLNPQTMNDLF
                     DHEIEGCFLYSRHWNPTNKYLAEALAKLEDSEAAIVTASGMGA"
BASE COUNT         1689 a          724 c          787 g         1657 t
ORIGIN      
        1 gctttattaa ttagaatagc atcagtcaat tcaattatgc cacgtttgat tccctgtaat
       61 tcatctcccg cgccggcaat caaaacgagt aaaaagaaat ctaccataga acgtacggta
      121 acttcactct gtccaacacc gactgtttca ataagaataa tatcaaatcc tgcggcttca
      181 caaacagtaa ttgtttcacg actttttctt gttactccac caagattgcc cccggaaggt
      241 gatggacgaa tgaaacaatt tttttctttg gataaatttt ccattcgtgt tttatcgcca
      301 agaatacttc ctttggttac ggtactgctg ggatcaacgg taagtactgc aactttgtgt
      361 ccttgtttaa tcaaatacat accaagtgct tcgatcaatg tacttttacc agcgcccggt
      421 actccggtga ttccaattct taaagagtta ccagaatggg gcaaaagttt ctttaaaact
      481 tcctgtgcta aattatggtg tgaaggatta ttgctttcga ttaaagttat ggttcgtgcc
      541 agaatatttc tatcaaaact tacaactcca tttacgtaat catcagcaga aagatttttg
      601 cgttttaaat tacttcccgc cttatttgaa cttaagtctt ttacaccttt cacaactcgt
      661 acagcaaatt catctcctgc attttcagga gtccagtcgg gtttgtatga agtattttta
      721 tttgatgatt gttttttcat atatcaaaaa tagatattca aaaaataata ttataaagga
      781 tttaattgta aatgtttatt tagaatttca tcataataat ttacacagaa acattttttt
      841 gagtttataa tatttaatat ctttaactca gaccaagtta attttagata aaaatggaac
      901 tgttaaagta taatatagtt taatcatttg aaattaatta aaaaatttaa tactaatcgt
      961 taaaaattat tgaaggaata aaatttatgt taaaagtgtt tttggttctt gttgggatat
     1021 ctttatttgt aaatgcctgc agcaccgcag gttcaattac aaaattagat gatacggaat
     1081 ggagcgttac ttctcttttg ggcaaaacct taaaagctga tgatatgatg aagggattac
     1141 catctcttaa ttttagcgag aatggaaaat tattcggatc aaccggatgc aataatttta
     1201 caggttcata caaacttgat gggacaaagc tttctattga tccgggggca atgactaaaa
     1261 tgttttgtcc gggagacact gaacaggatt ttctaaaagc gattagccgg ataacaaatt
     1321 ataaactgga agggaacact ttaaatctgt tagatggtac aaatgctgtt atgaaattag
     1381 ctcccaaatc aaagtaatat ttttcatttt attattgtta ttttttaagc ccagaattaa
     1441 aaattattct gcgctttttt attatgtatc aatttcattt taatattcag actttgttga
     1501 tgaagttata agagaagaaa ttgaaaccag tattagtgaa aataaataaa cttttaaacg
     1561 gtttgtttaa tgccagcgat tattcagaaa aggttaaagc ttgagatttg aaactaaaaa
     1621 caataaatat aatatcgggc ataattacct caataaatgt atcgttatct taagaatatt
     1681 atgaatacca tcgaacaaga attaaaaaag gccataagtt atattgaaca aaataaatct
     1741 actgaaattt ctatctcctc aatagaagag caattaaagt tattttattc aggagtccat
     1801 atcccggctt tagcaaaacc ttgtatggtt ggtgatggta ttcacgggat aaaggatcta
     1861 gaaattgatg agatattggc gctttataat gaagctgcta atgctggcag gctgattaaa
     1921 tttgtacccg cttcgggggt cgcttcgcgt atgtttcaca agctgcaatc tgttttaatt
     1981 cgttttaatg attttacttt agatgatctt aaaaaaaaat ctgaagatga aaaagaatgt
     2041 aaaagtgttt tagaaatcct gatcaatctt aagaagtttg ctttttacaa tgagttaaaa
     2101 agtattctga atgttgacga agcaggactt atgaaaataa ttgatgaaac tcccgcggaa
     2161 atattaaaag caattttgtt tgataacggt ttaaattatt catcaaaacc aaaaggagca
     2221 ataaaatttc atagatacga aaatgaaagc agaacagctt ttgaagagca aatttatgaa
     2281 gcattacatt acatttctga taaagaagga aatgtgaaaa ttcattttac aatttctgaa
     2341 gaacacacaa acttatttaa tacaattatt gatcaattaa aatcaaatat ttctaatcaa
     2401 tataaagtgg atgtaacgta ttcatttcaa aaaaaatcta catatactat tgcagttgat
     2461 attgagaaca aattgatatt tgataaatcg agacaacttg ttcgaagacc tggtggtcat
     2521 ggtgcattat tagaaaatct aaataatttg aaggcagatg ttgtaatcat aaaaaatata
     2581 gataatcttt caaaagaaaa tcttgcttct gatacaatcc tgtataaaaa attattaata
     2641 ggatatttaa tcaaagtgca gaagcaagtt tttgaaatgt tgtacgctct aaatgaaaaa
     2701 gattttgacc aaaaaagatt tgatgagatg ttagactttg ctgaaaatga tttatttata
     2761 atcaaaccaa cgggctttga aaactggagt gatgagcaaa aacaaaagtt cttatttagc
     2821 aaattaaatc gcccaattag ggtttgtgga atggtcaaaa acgagggtga acctggcggg
     2881 ggacctttct gggtgaatga caataatgga agtttatctt tacagatcgt agagcatgca
     2941 cagataaata aggatgatga aaatcaacaa aatatttttg agcaatcgac ccattttaat
     3001 cctgtggatt tagtttgcgg attgagggat tataaaggcc gcaattttga tctgcacaat
     3061 tttgttgatc ataaaagcgg catcattaca aaaaaatcta aagacggtat cgaactgaaa
     3121 gctcttgagt tacccggttt gtggaatggc agtatggctg attggattac gatatttgtt
     3181 gaagtgccta ttagtacttt taatccagta aaagaattaa atgatctgct aagaaaagaa
     3241 catcaaaatt aattttcacc actttacaat ttcttttaca ttacatacat tcgtatggta
     3301 tgaaaaagaa atcctttaga tctcaattat tcagaaaaga aattcaggta aatgatccgg
     3361 aatactggat taaagtttat gcagaattac ttttcagaat tgatgagctt gagatgcaaa
     3421 aatgtaattg ttttaaatgc caaactgatc ttgatagata tctaaatttc tttcaaaaat
     3481 tacgtgatga taaaacatat ctttatagat cgaagctgga ctccgcataa tcttaaaaat
     3541 ttgcatgtaa taattacctc gagaatttat tttacctttt attctctgag cgattaaagt
     3601 ttttcaagga tttttgaaac aaatcatgtg gcactaagct tgtgattttt tttagaaaat
     3661 ttcaatcaga aaggaatatt tgtaatgggt tttacagaaa ttggtgtgat tttattctta
     3721 gttgcacttt tagcttttgg ctttatgaat tcaagaaaag tggctcagga attaagaaaa
     3781 ttggaatctg aaaaataaat tttcaatttc ctcgttaatt atctactaat tatctctgtt
     3841 gtatttcctc ttccgataat tactctatct gctatgttgt tgaaaagtcc aacttcaaca
     3901 attccaggga ttagttttat gtctttctca agttttatag aatcttcaat ttcatttaaa
     3961 tgaatatcga ttatttcatt tcctgaatct gtaaagtaat cgttgttgcc attttttctt
     4021 cttacaggat tataaccgcg ttccataaat cttttaataa tatgattaga accaaaaggt
     4081 attacttcaa ccggcagagg aaactttcct aatttctcaa catactttga tgaatcaaca
     4141 atccagataa cttttttaga tgacgctgca acaatctttt caaacaataa agcaccgccg
     4201 ccgcctttaa ttccgtttaa atttttatca acttcatcgg cgccatcaat tgttaaatca
     4261 ataaaatcaa cctcgtttaa atttacaatt ttgattccca aactttttgc taattcactg
     4321 gtttggttag aagtggaaac gcattttact tctaatcctt gtttaactaa ttcagcaagt
     4381 ttattaacta aaaagaaaac agttgaaccg gtaccaagtc caagtgtcat cccatcttta
     4441 ataaactcaa tggatttttc tgctgcaagt tttttttcgt tcattttcat caattattag
     4501 taaataaaat ctttaactaa tcgtaattta gtaatagcaa aattaaaata gttagacaat
     4561 cttaaaatga ttataaaaaa taagaggtaa agttatgcat cattacgatc cgagcattga
     4621 aatacaagat tatttagttt ttggggagta tggagatgtt aatccatcta tcaccgattc
     4681 ttctacttac actttcctta atccgcaaac gatgaacgat ttatttgatc atgaaataga
     4741 aggatgtttt ttatattcac gtcattggaa tccgactaac aaatatcttg ctgaagcact
     4801 tgcgaagctg gaagactctg aagcagcaat tgtaacggca tccggaatgg gagcaat
//
LOCUS       DASWBY010000460         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_54228, whole genome shotgun sequence.
ACCESSION   DASWBY010000460 DASWBY010000000
VERSION     DASWBY010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54228"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VF870_08230"
     CDS             complement(<1..544)
                     /locus_tag="VF870_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX9252215.1"
                     /translation="MGYELLDQIKAKAKSKNKTIILPESHDDRVLKAAEILTKKKICK
                     VITLGNPAKVNEDAKRLGVDLTGVEVIDQLTHPKFDEFANIYYELRKKKGMTPEQARE
                     VIKRDLFFAAMMLREGLVDGSVAGSFASTADVMKAGIQIVGMPQGISIVSSFFLMLFK
                     DRAFSFADCAVVPNPDAEQLA"
     gene            787..1488
                     /locus_tag="VF870_08235"
     CDS             787..1488
                     /locus_tag="VF870_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX9252216.1"
                     /translation="MIVQNIANLRQKIEEACKRLGRNPDEVKLIAVSKYFGVDAIFEA
                     KNCGLTDFGENRAQELTLKYEKLGNSVVWHMIGTLQKNKVKYAVKAAELIHSVDSIEL
                     VEEINNCASKFGKIQKILLEVKTSEEETKSGLETENEIMSLVKRCFELKNIDLKGLMT
                     MAPLTEDANIIRKSFRDLRNLKDQINNKGYNLTELSMGMTSDFEIAIEEGATMIRIGS
                     AIFGDRDYSKDWRQV"
     gene            1485..>1722
                     /locus_tag="VF870_08240"
     CDS             1485..>1722
                     /locus_tag="VF870_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX9252217.1"
                     /translation="MKISPIEIRQQEFTKKMRGYDPDEVQNFLESLAEELDKLNNENE
                     SLKIEFESLTEQINEYKKIEKNLQDTLLSAQESST"
BASE COUNT          611 a          269 c          301 g          541 t
ORIGIN      
        1 ctgcaagctg ttctgcatca ggatttggaa caacagcgca atctgcaaaa ctgaaagctc
       61 tatctttgaa caacattaaa aagaagcttg atacgattga aattccctgc ggcatcccaa
      121 caatttgtat tcctgccttc attacatcag cagttgaagc gaatgaaccg gcaacacttc
      181 catcaaccaa accttcacgc agcatcattg cagcaaagaa aagatcacgt tttattactt
      241 ctcttgcttg ttcaggagtc attccttttt tctttcttaa ctcgtaataa atatttgcaa
      301 actcatcaaa ctttggatga gttaattgat caattacttc aacaccggtt aaatcaactc
      361 caagtctttt tgcatcttca tttacttttg caggattacc aagtgtaatt accttacaaa
      421 tttttttttt ggttaaaatt tctgcggctt taagaacccg atcatcgtga gattctggaa
      481 ggatgattgt tttgtttttt gatttagctt tagctttaat ttgatctaat aattcgtagc
      541 ccataatatc tccggaataa attgtgaata ataacaatca aaattactaa aatatttata
      601 attagttttt ttaagcactt caatcagtta aaggttatat ttacagggtt aaaacaaatg
      661 attgtttacg gaaattactg acgatcctct gactttatgc tgttttgtca cgatacgacc
      721 gtgacagtta attatttctt tgttttttac aatttatcag gataaattca cggcctaatt
      781 tttaaaatga ttgtacaaaa tattgccaat ttaaggcaaa aaatagaaga agcttgtaaa
      841 agattaggaa gaaaccctga tgaagttaaa cttattgcgg tttctaaata tttcggggtg
      901 gatgcaatct tcgaagcaaa aaattgcgga ttaaccgatt ttggtgaaaa tcgtgcacag
      961 gaacttacgc ttaagtatga aaaacttggc aattcagtag tttggcacat gatcggtact
     1021 cttcaaaaga ataaagtcaa atatgctgtt aaagctgctg aattgattca ttcggttgat
     1081 tcaatagaac tagttgaaga aattaataac tgtgcgagca aatttggcaa aattcaaaag
     1141 attttgctgg aggtgaaaac ttctgaagaa gaaacaaaat caggacttga aactgaaaat
     1201 gaaattatga gtttggttaa acgatgtttt gagctaaaga acatcgatct taaaggttta
     1261 atgacaatgg cgcctttaac cgaggatgca aatattatta ggaaaagttt tagagattta
     1321 cgtaatttga aagatcaaat taacaataaa ggttacaatc ttacagaact ctcaatggga
     1381 atgaccagtg attttgaaat tgcaattgaa gaaggtgcta caatgataag aatcggaagt
     1441 gcaatttttg gagacagaga ttattcaaaa gattggagac aagtatgaaa atatctccta
     1501 tagaaattcg tcagcaggaa ttcacaaaaa aaatgcgtgg atatgatcct gatgaagttc
     1561 agaactttct tgaaagttta gctgaagaac ttgataaact taacaatgaa aatgaatcac
     1621 ttaagattga atttgaatct ttaacggaac agattaacga atacaaaaaa attgaaaaaa
     1681 atctgcagga tacactcctg agcgctcagg aatcctcaac aa
//
LOCUS       DASWBY010000461         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_54317, whole genome shotgun sequence.
ACCESSION   DASWBY010000461 DASWBY010000000
VERSION     DASWBY010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54317"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF870_08245"
     CDS             <1..216
                     /locus_tag="VF870_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252218.1"
                     /translation="KYSDVYYKSEYHLDLSVNQRITDKFKIFLQLNNLTDQFENESFG
                     DPAESFSKWTQWAKFGPYGTLGVSFKL"
     gene            387..>1682
                     /locus_tag="VF870_08250"
     CDS             387..>1682
                     /locus_tag="VF870_08250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9252219.1"
                     /translation="MLKKIVKFFATGEDKALLEDENQIKKIYENKRWSVFISLILGYA
                     FFYTCRLGLSVAKKPMLDSGVLSVSEMGIIGSVLLYVYAVGKFTNGILSDRANIKRFM
                     SIALLISAILNILFGLTNLFIAFVILWGLNGWFQSIGSAPSVVSICQWYSNRERGTRY
                     GIWAGAHNIGEGLTFVVTAAFVSYFGWHAGFIGPGIIGVFVSLVLYNTLADRPQTYGL
                     PHVADYKKDYSAGKPISEEVWKLQRMVLKNPYVWILGLSSALMYVARYAINNWAILFL
                     QESKHYELMDASAVMSAYPIMGFLGAAFSGFFSDKIFNSRRNIPTLLYGVIETGAIFM
                     LFMIPPGHVWLDTIAMGLFGFGIGGLIVFLAGLIAIDIVPKAAAGTVKGLIGLFSYIG
                     AATQDWISGFLIENSKIMENGKAIYNFDVAFYFWIGASLL"
BASE COUNT          490 a          260 c          354 g          578 t
ORIGIN      
        1 aaatattccg atgtttacta caagagcgaa tatcatcttg atttatctgt gaatcaaaga
       61 attaccgata aatttaaaat atttctgcag ttgaacaacc ttactgacca atttgaaaac
      121 gaatcatttg gcgatccagc agaaagtttt tctaagtgga ctcaatgggc aaaattcggt
      181 ccttatggta ctctgggtgt ttcgtttaaa ttatagactg ctttttttct attagcggcg
      241 agctgaaaag gttcgccgct ttattattca ttgacgattc atatggttct aaaaatcctg
      301 tatagcttac tcaaagatga tatcatatgt cttaatcgcc cttaaaaaaa tagtctgtga
      361 ctttaaaaac taaaactgag aagaaaatgc tgaagaaaat agttaagttt tttgctacag
      421 gtgaagataa agccctactc gaagatgaaa atcaaatcaa gaaaatttat gaaaataaac
      481 gctggagtgt ttttatttcg ttgattcttg gctatgcttt cttttacacc tgcaggttag
      541 gattatccgt tgctaaaaaa ccaatgcttg attctggggt tttatctgtg tctgagatgg
      601 ggatcatcgg ttcagtatta ttgtatgttt atgcagttgg taagtttacc aatgggatcc
      661 tatctgatag agcaaatata aaaaggttta tgtcaattgc gctacttata tcggcaatac
      721 tgaacatatt attcggtctg acaaatctat tcattgcatt cgttatactt tggggattga
      781 atggttggtt tcaatctatt ggttctgctc caagcgtagt ttcaatatgc cagtggtaca
      841 gtaatcgaga aagaggtaca agatatggta tctgggctgg tgctcataac atcggagaag
      901 gacttacatt cgttgtcact gccgcatttg taagttactt tggatggcat gcaggtttta
      961 taggtcctgg tattattggt gtatttgtat cattggttct ctataatact ttagcagatc
     1021 gtcctcaaac ttacggtcta ccacatgttg ctgattataa aaaggattac tctgctggaa
     1081 agcccatttc cgaagaagtc tggaagctgc aaagaatggt tttgaaaaat ccatatgtat
     1141 ggattttggg attgagcagt gctctaatgt atgtggcaag atatgcgata aataattggg
     1201 cgattttatt tttgcaggaa tcaaagcatt atgaattaat ggatgctagt gctgtaatga
     1261 gtgcctaccc aataatggga tttttgggag cggcattttc tggatttttt tccgataaga
     1321 ttttcaactc tagaagaaat attccaacgc ttctttatgg cgtaattgaa actggcgcca
     1381 tatttatgct ttttatgatt cccccaggac atgtttggtt ggatactata gcaatgggtt
     1441 tattcggatt cggtattggc ggtctcattg tttttctagc cggattaatt gctatcgata
     1501 ttgtccccaa agctgcagct ggtacagtaa aaggattgat tgggctgttt agctatattg
     1561 gtgcggcaac acaagattgg attagcggtt ttctaattga aaacagtaaa attatggaaa
     1621 atggaaaagc aatttacaat tttgatgttg cattctactt ctggatagga gcatcattac
     1681 tt
//
LOCUS       DASWBY010000462         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_169028, whole genome shotgun sequence.
ACCESSION   DASWBY010000462 DASWBY010000000
VERSION     DASWBY010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169028"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VF870_08255"
     CDS             complement(<1..1007)
                     /locus_tag="VF870_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX9252220.1"
                     /translation="MSQEKETTLKTVTPDEYEKAKNKFNDEEYSQEEFMSLAKLYLDS
                     FRDVKEGEMIKGKVVRIMGDNVIVDVGFKSEGTIPRNEFFEGVDLKVGQEIEVVLESV
                     EDQEGNLVLSKQRADFLRIWEKVLRAHDTGEIIEGKIVKRIKGGMVVDLLGMEAFLPG
                     SQIDIRPIRDFDAFVGQTMDFKVVKVNVPTENVVVSHKVLVEEEISDQRNAILNSLEK
                     GQILEGTVKAITDFGVFVDLGGVDGLIHITDLSWGRINHPNEVVKLDEVIKVVVTDFD
                     EEKRRISLSLKKLLPHPWDQIQEKYNIGDKVSGRVVSLTDYGAFIEIEKGIEGLIHNS
                     E"
     gene            complement(1100..1789)
                     /gene="cmk"
                     /locus_tag="VF870_08260"
     CDS             complement(1100..1789)
                     /gene="cmk"
                     /locus_tag="VF870_08260"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX9252221.1"
                     /translation="MSKKLIIAIDGPAGSGKSTTAKLVAKRLNYLYIDTGAMYRAVTL
                     FVLRNNLMGKTNEIIGFANGLNIVLSFIDGETNITVNGEDVSKEIRSFDVNSNVSEIS
                     CIEGVRKILVKKQQEMGKNGGVVMEGRDIATVVFPNADVKIFLTAGIDERAERRAKEF
                     SDKGTDVPIDKVKENLKNRDFIDSNREVSPLTKAADSIEVDTSNTTIDEQVNLILDEV
                     KIIADKKNIRI"
     gene            complement(1868..>2612)
                     /locus_tag="VF870_08265"
     CDS             complement(1868..>2612)
                     /locus_tag="VF870_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX9252222.1"
                     /translation="TKYANYIQTFSKGDLLRILNYLSKLQQELRYSNNQKLKIEIALT
                     NLVGLEKSATLSEIISGLESANVEIKSSPDASVTRESSSKSYSSKQIIPEKKREIKTE
                     LAPKTEVFVPPIKETLSGIQSDLNFEIITQKWQSFVDDLCKEKSFLLTPLFSNVKPIN
                     FDGTNLHLESADPTTESSLRLHQDYINKKSRDFFGKKVNIKFGADESTKVKLAPKNEP
                     KSVKSSKTSKIADPYEKIIIDDLGGREIN"
BASE COUNT          779 a          499 c          374 g          960 t
ORIGIN      
        1 atttctgaat tatgaataag accttcgatt cctttttcta tctcaataaa agcgccgtaa
       61 tcagtaagag aaacaactct acctgaaact ttatcaccaa tattatattt ttcctgaatt
      121 tgatcccaag gatgtggaag aagtttttta agagaaagtg aaattcttct tttctcttcg
      181 tcaaaatcag ttacaacaac cttaattact tcatcaagtt ttacaacttc gtttggatgg
      241 ttgattcttc cccagctgag atcagtaatg tggataagac catcgacacc accgagatca
      301 acaaacactc caaaatcagt aattgcttta acagtacctt caagtatctg tcctttttca
      361 aggctgttaa gtattgcatt tctttgatca gaaatttctt cctcaacaag tactttgtgt
      421 gatactacaa cattttctgt aggaacgttg actttaacaa ctttgaaatc cattgtctga
      481 ccaacaaatg catcaaaatc tctgattgga cgtatatcaa tttgtgaacc tggtaagaat
      541 gcttccatcc ctaaaagatc aacaaccatt ccgcctttaa ttcttttaac aattttacct
      601 tcgataattt caccggtatc gtgagctctg agaacttttt cccagatacg taaaaagtct
      661 gctctttgtt tgcttagaac aagatttcct tcctgatctt caacactttc aagaactact
      721 tcaatttctt gtccgacttt taaatcgacg ccttcaaaaa attcgttacg tggaattgta
      781 ccttctgatt taaaaccaac atcaacgata acattatcgc ccatgattct aacaactttc
      841 cccttgatca tctcaccttc tttaacatct ctaaatgaat ctaagtagag ttttgccaac
      901 gacataaact cttcttgaga atattcttca tcgttaaatt tattcttggc tttttcatac
      961 tcatcgggag tcacagtttt tagagtcgtt tctttttctt gggacattaa tcctcctaaa
     1021 aatatttttc tgtttttctg agtctcagcc ggaaagctca gtagaacaga ctatttagtt
     1081 taacattagt ttagccggct taaattctaa tatttttttt atcagctata attttaactt
     1141 catctaaaat caaattaact tgttcatcaa ttgtggtgtt ggaagtatca acttctattg
     1201 aatcagcagc tttggttaat gggcttactt cacggtttga atcgataaag tctctatttt
     1261 ttaagttctc ttttacttta tcaatcggaa catcagttcc tttgtcagaa aactctttag
     1321 cacgtctttc agctctttca tcaataccag cagttagaaa aattttaaca tcagcatttg
     1381 gaaatacaac agttgctatg tctcttcctt ccataacaac accaccattt ttgcccattt
     1441 cctgctgctt cttaacgagt atttttctta caccctcgat acagcttatt tcacttacat
     1501 tactgtttac atcaaaagat ctaatttctt tagaaacatc ttccccattt acagtaatat
     1561 tagtttcgcc atcaataaaa cttagaacaa tattcagacc gtttgcaaac ccgataattt
     1621 catttgtttt gcccatcaga ttatttctca aaacaaataa tgtaaccgcc cggtacatcg
     1681 cacctgtatc aatgtaaaga taattcaatc ttttagctac aagttttgcg gttgtacttt
     1741 taccggagcc tgctggtccg tctatggcaa tgattaactt ttttgacata ggaaacaaaa
     1801 atagttataa ctatttaatt atcaaacgct tagggggatt gaaaaagtgg aaaataagag
     1861 aataaactca gtttatttct cgtcctccaa ggtcatcaat aataattttt tcgtaaggat
     1921 cagcaatttt tgaagttttt gaacttttta cggatttggg ttcattttta ggtgcaagtt
     1981 ttacttttgt tgattcgtcc gctccaaact taatattcac ttttttaccg aaaaaatcgc
     2041 gcgatttttt gttaatataa tcctgatgta atcgtaaaga agattcagta gttggatctg
     2101 ctgattccag gtgaagattt gtcccatcaa aatttatggg ttttacgttg ctaaaaaggg
     2161 gagtaagcaa aaaagatttt tccttacaaa ggtcatcaac aaaactttgc cacttctgtg
     2221 ttattatttc aaaatttaaa tctgattgaa ttcctgatag cgtttctttt attggaggaa
     2281 caaaaacttc agtcttagga gctagctctg tcttgatctc gcgttttttt tccggaataa
     2341 tttgttttga tgaataagat tttgaactac tttccctggt aaccgaagca tcaggactgc
     2401 ttttgatttc aacattcgct gattctaatc ctgagataat ttcagaaaga gttgcagatt
     2461 tttctaatcc gacaagattt gttaacgcaa tttctatttt aagtttttga ttatttgaat
     2521 atctcagttc ttgctgaagt ttacttaagt aatttaatat tctaagtaaa tcacctttag
     2581 aaaaagtttg aatatagttt gcatattttg tt
//
LOCUS       DASWBY010000463         5606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_245090, whole genome shotgun sequence.
ACCESSION   DASWBY010000463 DASWBY010000000
VERSION     DASWBY010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245090"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /gene="rfaE1"
                     /locus_tag="VF870_08270"
     CDS             <1..990
                     /gene="rfaE1"
                     /locus_tag="VF870_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX9252223.1"
                     /translation="KISEKRLKQLKANFNGKRIAIIGDMMLDIYFWGDVKRISPEAPV
                     PVLEVEDEFYRFGGAANVALNISSLNGIAEPVGIIGYDNFGTIFNSLLTERKISHHGI
                     IEDDSRPTTAKTRVIADSQHIVRIDKESKETIGQNIQNKIFDYVKSIIKNIDGIILQD
                     YNKGVLSSTLISQLIELANKNKILITVDPKFNNFYEYKNVTVFKPNRKEAEDILGMKI
                     KTDADVSFAGNTLLKKLNAKNILLTLGEDGIAVFEKGKPEKRMPTKARKVADVSGAGD
                     TVISTLTMALAAGANIYEASYLANYAGGIVCEEVGIIPIGIDKLFDTVMRENK"
     gene            987..1466
                     /gene="rfaE2"
                     /locus_tag="VF870_08275"
     CDS             987..1466
                     /gene="rfaE2"
                     /locus_tag="VF870_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX9252224.1"
                     /translation="MSIIKSREEIKRIISQLKKEKKKVVFTNGVFDLIHAGHVDYLVK
                     AKKLGDVLVLALNTDSSVKRIKGDKRPILQQDERAFIVSNLKPVDYVTFFDEDTPAEI
                     ISDLIPDVLVKGADWAIDKIVGREVVEANGGEVKAIKFVNEQSTSKVIKIIAERYKD"
     gene            1466..>5606
                     /locus_tag="VF870_08280"
     CDS             1466..>5606
                     /locus_tag="VF870_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252225.1"
                     /translation="MDEKKIQEETKKIIGKGRRALRYGVNIFVYTFVGIFMLLMVFFG
                     VSQTSIFKNWLRNTVVETVNDGIKGKLSIGEIDGTIFTSLLIKNVTLTSLQNDTVVSA
                     ENIELRTSPLKILFKNIYVRKFELKDAKLKLVVEKDGQLNLLKIFPPSNEPEDTTSSE
                     FPFSIEAADFALTNVDFSLQRYDKVGSTQYYQSLTTDDLRITDLNVSFNAFADLNKYT
                     YRLTINNISFNPNFNFFQLQHLSGSILLTPQLAGINKLHLITRDSDFELSAAVLGVDF
                     LKNFSVEKLETAPVRLSLNSTKLNVDDVTTYVPAMKMFDGIISTQLEGSGTFNDLVLK
                     KFTINYNNTSLKATGVLKNLLDTDKMNFDISITDSYIDPSDPNKLLRDLEVPEYIEFG
                     LFKIDTLKYFGGPLDFKTTFAIRTDKGNMNGSANIDLRSAEMIYDAMLKTKNLDISPF
                     TSISTDMNSEIKIKGVGFNPQKMKLDFSMDAFSSRFGKKYFKDINIKSNAENGLLTTT
                     LSLSADSTSADLLATLDFKNADDPTYSIKGKLNGINLAKLLDNEALASEYNLSLDASG
                     QGFNPDSMDLFLVTDIQNSRFADFDIDSTRLIMDIRRNDGGKKIINIISDIADLTISG
                     DYLITSLGDVLSREAEIISEAITKKIDPIFENDSVKNLDNKTIVSTNYNSVKDFSLEY
                     LLDFKEAVVINVGKDQFALDGRINGNMDSSHDSLIVSLNSKIDYLKFWNDEEVYFLIN
                     TEINSNLSNHLLNGYSENLEASIEFNSDRIYASGNIYDVRSKVTLAANKLNISAAGRY
                     EDDISGKLVSNIIFEDSVLQLDIKGLNLNYKNFNIKNSNDLLVKYSKDNISFDKFLLK
                     AADGILNIDGTFGKSGDHSLNISLEGLSGKKLTEDIISSVGQKNFDADINIKGLVTGN
                     FINPKFSIDASAKNIFYGENNFGSLLSVFDYENNKLNTDIRFIDSTNNYNNPAMLITG
                     YIPITLTSSKDSVNNSNEKIELTIESDEFDLGSLRNSMPYVDFQKGKLETDIYISGTI
                     SKPVAMGYFSINDARFKVTNNNLDYDLNTKIWIDDEDITIESITLQNVFGTKQGGTLK
                     GEGFVKLDEFKLDSTFIKVNGDLKVLDKISKSSSPFIYGDVAFKTRGDIVYSAKRGKS
                     YVNLPISVTVADIVIPLTKSAYSSSSGFIYEYAQPSKEVDKLIVELDSLIETTHKKDS
                     IESKNEKAAKFDYTIDIKLDTEAEVVVVLSKELDQNLVAILDGNFFLESIDGKTKSGG
                     ELKVLEGSSLSFIKTFSASGNIRFDKIDNPIIDITSTYKDYYYPPNSSGGQTEQEVAV
                     KIKLKGPLSELNQNFIKDENNIGVYMGRQAIEEDKKDPTKNATDAMYFLIIGKFPGDD
                     NS"
BASE COUNT         2038 a          744 c         1021 g         1803 t
ORIGIN      
        1 aaaatttctg aaaaaagatt aaagcagtta aaagcaaatt ttaatggtaa acgtattgcc
       61 attattggcg atatgatgct tgatatttat ttctggggtg acgtaaaaag aatttctcca
      121 gaagccccgg ttccggtttt agaggttgaa gatgaatttt atcgttttgg cggtgcagca
      181 aatgttgcat taaatatctc ttctttaaat ggaattgctg agccggtagg cataattggg
      241 tacgataatt ttggaacgat ttttaattct cttttgacag aaagaaaaat ttctcatcac
      301 ggaataattg aagacgattc cagaccaacg actgctaaaa caagagttat tgcagatagt
      361 cagcatatcg taagaatcga taaggaaagt aaagaaacca ttgggcagaa tattcaaaac
      421 aaaatatttg attatgtaaa aagtataata aaaaatattg atggaattat acttcaggat
      481 tataacaagg gagttttatc ctctacatta atttctcagt taattgaact ggcaaacaag
      541 aataaaatac taattactgt cgatccaaaa ttcaataact tttatgagta caaaaacgta
      601 actgtattca aacctaacag aaaagaagct gaggatattc tgggaatgaa aataaaaaca
      661 gatgctgacg tttcttttgc aggaaacaca ctgttaaaaa aactaaatgc aaaaaatata
      721 ttgcttactc tgggagaaga tggcatcgca gtttttgaaa aaggcaaacc ggaaaagcga
      781 atgccgacaa aagcaagaaa ggttgctgat gtttcgggtg caggtgatac ggttatctca
      841 actttaacga tggcacttgc ggcaggtgca aatatttatg aagcatctta tttggcaaac
      901 tatgcaggcg gaattgtttg tgaagaagtt ggaattatcc caatcggaat agataaactt
      961 tttgatactg ttatgcggga aaataaatga gcatcattaa atcaagagaa gaaataaaaa
     1021 ggattatatc tcagttaaag aaagagaaaa agaaagtagt ttttacaaat ggtgtctttg
     1081 atttaattca tgccggacat gttgattatc tggttaaagc aaaaaaattg ggtgatgttt
     1141 tggtactggc tttaaataca gattcatctg taaaaagaat taaaggagat aaacgcccta
     1201 ttttacaaca ggatgaacga gcatttattg tatccaatct taagccggtt gattatgtga
     1261 ctttttttga tgaggatacc cctgcggaaa ttattagcga tctgattcct gatgttcttg
     1321 ttaaaggtgc ggattgggca attgataaaa ttgttggaag agaagttgtt gaagctaacg
     1381 gaggtgaagt aaaagcaata aaatttgtta atgagcaatc aacatcaaaa gttattaaaa
     1441 ttattgcaga acgatataaa gactagtgga cgaaaaaaaa atacaggaag aaacgaagaa
     1501 aattatcggt aaaggcagaa gagctttacg ttatggtgta aatatttttg tttacacttt
     1561 tgtaggcatc tttatgctgc ttatggtttt ctttggtgtt tctcaaacct ctatttttaa
     1621 aaattggctg cgtaatactg tagtagaaac tgtaaatgat ggaattaaag gtaaactatc
     1681 tataggcgaa atagatggaa caatattcac atcgctgctg attaaaaatg ttacattaac
     1741 atcacttcaa aacgacactg ttgtatccgc agaaaatatt gaacttagaa caagcccgtt
     1801 aaaaattctg tttaaaaata tctatgttag aaaatttgaa ttaaaagatg ctaagttaaa
     1861 actggtagta gaaaaagacg ggcaattaaa cttacttaaa atatttccac catcgaatga
     1921 acctgaagat acaacatctt cagaattccc attttctatt gaagctgcgg attttgcact
     1981 tactaatgtt gatttttctt tgcaaaggta cgataaagtt ggcagcacac aatattatca
     2041 gagtttgaca actgatgatt tacgaattac cgatctgaac gtttccttta atgcttttgc
     2101 cgatctgaat aagtacactt acagacttac aattaataat atttcattta atcctaactt
     2161 taattttttt cagctgcagc atttaagcgg cagtattctg cttacgcctc aattagccgg
     2221 aattaacaag cttcacttaa ttacaagaga ttctgatttt gaactgagtg ctgcggtttt
     2281 aggtgtggat tttctaaaga atttttctgt tgaaaaactt gaaactgcac cagttagact
     2341 aagtttaaat tcaacaaaat taaatgttga tgatgttaca acttatgttc ctgcaatgaa
     2401 aatgtttgat ggaataatat caactcaatt agaaggttca ggaactttta atgatttagt
     2461 gctaaaaaag tttactatta attataataa cacttcactt aaagcaaccg gagttcttaa
     2521 aaatttgttg gatactgata aaatgaattt tgatataagt attactgatt catatataga
     2581 tccatcagat cctaataaat tacttagaga tttagaagtg cctgagtaca ttgaatttgg
     2641 tttatttaag attgatacat taaaatattt tggcggaccg ttagatttta aaactacttt
     2701 tgctatcaga actgataaag gaaatatgaa tggttcggct aatatcgatt tacgttctgc
     2761 ggaaatgatt tatgacgcaa tgctaaaaac aaaaaatctt gatatcagtc catttacatc
     2821 aatttcaact gatatgaatt ctgaaataaa gataaaggga gtcggattta atcctcagaa
     2881 gatgaaactg gatttttcaa tggatgcctt ttcttccaga tttggaaaga agtattttaa
     2941 agatattaac atcaaatcaa atgcggaaaa tggattactt acaacaactc tatctctttc
     3001 agcagattct acctctgcag atttactggc aactttagat tttaaaaatg ctgatgatcc
     3061 cacttacagt ataaagggga aactaaatgg tataaactta gcaaaactac ttgataacga
     3121 agcacttgca tcagaatata atctttcgct tgatgcaagc ggacagggat ttaatcctga
     3181 tagtatggat ttatttctgg ttacagatat tcagaactca agattcgcag atttcgatat
     3241 agatagtaca agattgatta tggatatccg tagaaatgat ggcggtaaaa aaataataaa
     3301 catcatctca gatattgctg atcttacaat aagcggtgat tatctcataa cttctttagg
     3361 tgatgtgctt tcacgggaag cagaaataat tagtgaagcg ataacaaaaa aaatagatcc
     3421 aatatttgaa aatgattcag taaaaaattt ggataacaaa actatagtat caactaatta
     3481 caattcagtt aaagattttt ctctggagta tttactggac tttaaagaag ctgtggtaat
     3541 aaatgttggt aaagatcagt ttgcactgga cgggcggatt aatggaaaca tggattcatc
     3601 tcacgattcc ttaattgtct cgcttaattc aaaaattgat tatttgaagt tctggaatga
     3661 tgaagaagtt tactttttaa taaacaccga gattaatagt aacctatcaa atcatttgct
     3721 aaatggatat tctgaaaatt tggaagcaag cattgaattt aactccgata gaatttatgc
     3781 aagcggtaat atctatgatg tgagatcaaa agttactttg gctgccaaca agttgaatat
     3841 ttctgctgct ggcagatatg aggatgatat ttctggtaaa ctcgtttcaa atattatttt
     3901 tgaggatagt gtacttcaat tggatatcaa gggactaaat ttaaattata aaaacttcaa
     3961 tattaaaaac agtaatgatc tcctggttaa atattcaaag gacaatatca gctttgataa
     4021 atttttatta aaagctgctg atggaatcct taatattgat ggaacattcg gtaaatctgg
     4081 agatcatagt ctaaatattt ctttggaagg actaagcgga aagaaactaa cagaggatat
     4141 tataagcagc gttggtcaga agaattttga tgccgatatt aatattaaag gattagttac
     4201 aggtaatttt attaatccca agttttcaat tgacgcatca gccaagaata ttttttatgg
     4261 tgaaaataat tttggttcat tgctttctgt ttttgattat gaaaacaata aattaaatac
     4321 agatattaga tttatagact caactaataa ctacaataat cctgcgatgt tgattactgg
     4381 atatattcca ataactttaa catcgtctaa agactcagtt aataattcaa atgagaagat
     4441 agagcttacc attgaatctg atgagtttga tttaggttct ttaagaaatt ctatgccgta
     4501 tgttgatttc caaaaaggaa aacttgaaac tgatatttat atatcgggaa caatttctaa
     4561 acctgttgca atgggatact tcagtattaa tgatgcaaga tttaaagtaa ctaataataa
     4621 tcttgattat gatcttaata ctaaaatctg gatagatgat gaagatatta ctattgaaag
     4681 tattacgctt caaaatgtat ttggaacaaa gcagggcggc acattaaagg gtgaagggtt
     4741 tgtaaagtta gatgagttta aattggattc aacatttatt aaggtaaatg gtgatcttaa
     4801 agttctggat aagatctcaa aatcatcaag tccttttatt tatggtgatg ttgcttttaa
     4861 aacaagaggc gatattgtgt attctgcaaa gcgtggaaaa tcttatgtta atcttccaat
     4921 ttcagtaact gttgctgata tagttattcc tcttacaaag tccgcatatt caagttcttc
     4981 agggtttata tatgagtatg cacagccatc gaaagaagtt gataaactga ttgttgaatt
     5041 ggatagttta attgaaacca cacataaaaa agattcaata gaaagtaaaa atgaaaaagc
     5101 agctaagttt gattatacaa tagatataaa acttgataca gaagcagaag tagttgttgt
     5161 attgtccaaa gagcttgatc aaaatttagt tgcaattctt gatggtaatt tttttcttga
     5221 gtcaatagat ggcaaaacaa aatctggagg cgaacttaaa gtacttgaag gatcgagtct
     5281 tagttttata aaaactttta gtgcttctgg gaatataaga tttgataaaa ttgataaccc
     5341 aataattgat atcacatcta catataaaga ttattattat cctcccaatt catctggtgg
     5401 tcaaactgaa caggaggttg cagttaaaat aaaattaaaa ggaccattga gtgaattgaa
     5461 tcaaaatttt attaaagatg aaaacaatat tggtgtatat atggggcggc aggcaattga
     5521 agaggataaa aaagatccta caaaaaatgc aacagatgca atgtattttt taataattgg
     5581 taagtttcca ggcgatgata atagtc
//
LOCUS       DASWBY010000464         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_318359, whole genome shotgun sequence.
ACCESSION   DASWBY010000464 DASWBY010000000
VERSION     DASWBY010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318359"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1870)
                     /locus_tag="VF870_08285"
     CDS             complement(<1..1870)
                     /locus_tag="VF870_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9252226.1"
                     /translation="MINSKRLYIQLYSLHGLIRGSDLELGRDADTGGQTKYVLELANI
                     LSTYPEVEKIELITRLINDKSVSSDYSVPIEKVNDKFDIVRIRCGGGKYIRKELLWNY
                     LEEFIDKSIKYLKAKKRLPDFIHSHYADAGYVCAELSKFFGLPFFHTSHSLGRDKQKK
                     MIEDGHSPEMIEKRYKMNIRIAAEEQIYFNADMIVTSTTQEIENHIYHYPGISQSKFK
                     VIPPGLNLEKFFPYNEERQFDKNTAMLIKQINREFLKFFVTVDKPLILTLCRPDRRKN
                     ISGLITAYGEDKQLQKKANLAIYAGVREDIQTMEDNEREVLTEILLLMDKYNLYGKMA
                     IPKKHDTDIEVPELYRIAARTNGVFVNASLSETFGLTLLEASASGLPVVTTKEGGPRD
                     IINNCKNGIKVDVSNPENISIALNKILDNKKMWREYSANGINNVKRSYTWQTHSKIYL
                     SEAKKILNEQSKVQNTFAETGRKLFDMEKLIATDIDYTLVGDDRSLNEFDEIIKNMHP
                     RIGFAVATGRVIDSTMEILKQKNIVMPDILITSVGSEIYYYYNGQLTYSKGWDAHISN
                     QWQRDKIVQLLSRYNFLKYQELKNQRKFKISYYTSNNKKNINMVRELLIKNKIKCNL"
     gene            complement(2036..2809)
                     /locus_tag="VF870_08290"
     CDS             complement(2036..2809)
                     /locus_tag="VF870_08290"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX9252227.1"
                     /translation="MKTKLDIAKNWLPRYTGTNIDDFGDYFLLTNFQNYVDKFAERFN
                     CEVKGIGRPMQTATNSNGLSIINFGIGSANAATIMDLLVARAPKGVLFLGKCGGLKRS
                     TEIGHFILPTAAIRGEGTSDDYKPSEVPALPSFKLHKFVSDKIIDHGMEYRTGVVYTT
                     NRRVWEWDEDFKQYLKKLSTIAIDMETATLFIVGLVNEIPRGALLLVSDIPMIPEGVK
                     TEESDKVVTERFADLHLEIGIEAMTELGVKGEQIKHFTY"
     gene            2888..>3601
                     /locus_tag="VF870_08295"
     CDS             2888..>3601
                     /locus_tag="VF870_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX9252228.1"
                     /translation="MNFKEAINHFPHIITEGSIIERLKREFQYPLDNLLSNAKMIYED
                     NGRVLLENIYREYINIAESSDLPIMLLTPTWRTNKERTKNANVDMKIINTDAFLFVEN
                     IRKSFGRFAEKIFIGGLTGCKGDAYKPEEALNQNEAYHFHKEQMQILADAGVDFLFAS
                     TLPALTEAIGIAKAMSETKKDYVISFVIRENGKLLEGTLLTDAIKIIDDSVLTPPLFY
                     LTNCIHPDVLHKSFFNLKDE"
BASE COUNT         1113 a          626 c          567 g         1295 t
ORIGIN      
        1 taagattgca tttaatttta tttttgatca gcagctctct taccatattt atattctttt
       61 tgttattaga tgtgtagtag ctgattttaa atttcctttg atttttaagt tcctggtatt
      121 ttagaaaatt atatcttgaa agaagctgca caattttatc tcgctgccat tgattagaaa
      181 tatgcgcatc ccaaccttta gaataggtga gctgaccatt gtaatagtag taaatttctg
      241 agccaacaga tgttattaaa atatcgggca taacaatatt cttttgcttt agtatttcca
      301 ttgtggagtc tattaccctg ccagtggcta ccgcaaatcc aatacgtgga tgcatatttt
      361 ttattatctc atcaaattcg tttagagatc tgtcatctcc aaccaatgta taatctatat
      421 cagttgctat aagtttttcc atatcaaaaa gttttcttcc cgtttctgca aacgtattct
      481 gaacttttga ttgttcattt aatatttttt ttgcttcact taaataaatt tttgagtgag
      541 tctgccaggt ataagatctt ttaacattgt taattccatt agcagaatat tctctccaca
      601 tttttttgtt atctaaaatt ttgttcaatg ctattgaaat attttcagga ttcgaaacgt
      661 ctactttaat accatttttg caattattta taatgtccct tggtccgcct tcctttgtgg
      721 taactacagg caaacctgaa gctgatgctt ccaggagagt caatccaaat gtttctgata
      781 aagaagcatt tacaaaaact ccattggtac gggcggcaat tctgtataat tcaggtactt
      841 caatatctgt atcgtgtttt ttgggtatcg ccattttacc gtatagatta tatttatcca
      901 ttaacaaaag aatttctgta agaacttcgc gttcattgtc ttccattgtc tggatgtctt
      961 cccgcacgcc ggcatagatt gctagatttg ctttcttttg aagctgctta tcttctccat
     1021 aagcagtaat caaacctgaa atattttttc ttcgatcagg cctgcaaaga gtaaggataa
     1081 gcggcttatc tactgttaca aaaaatttta gaaattctct attaatttgt tttattaaca
     1141 ttgcagtatt tttatcaaac tgtctctctt cgttatatgg gaagaatttt tcgagattta
     1201 atcccggcgg aattactttg aatttagatt gcgagatgcc tggataatga taaatatgat
     1261 tttcaatttc ctgagtggta cttgtaacaa tcatatctgc attaaaataa atttgttctt
     1321 ctgcagctat gcgtatgttc atcttatatc ttttctcaat catttcaggg gagtgcccgt
     1381 cttcaatcat ttttttttgt ttatctctgc ctaatgagtg tgaggtatgg aaaaaaggaa
     1441 gcccaaagaa ttttgaaagt tcagcacaaa catatcctgc atcagcataa tgactatgta
     1501 tgaaatctgg aagtcttttt tttgctttta agtatttaat acttttatca ataaactctt
     1561 caagataatt ccataaaagt tccttgcgga tatatttacc gccgccacat ctaattctaa
     1621 ctatatcaaa cttgtcatta actttttcaa tcggaacgga ataatctgaa gagactgatt
     1681 tatcattgat caatctggtt attaattcaa ttttttctac ttccggataa gtacttaaaa
     1741 tgtttgcaag ttccagcaca tatttggtct gtcccccggt atcagcatca cgtccaagtt
     1801 caagatcact tccccgaatc aaaccgtgca gactatagag ttgtatataa agtctttttg
     1861 agtttatcat aaatattctc tctctgtata atgaggtcaa gcatatacat aataacattt
     1921 attatgataa aatcaagtta acgtgatggg actaacacaa tgcgggtaat taaaagataa
     1981 aaaacgcgtg gattgtaatt atttaattaa tcaaactgct ttcagttgtt aataatcaat
     2041 aagtaaaatg ttttatttgt tctcctttta ctcctaattc tgtcattgct tcaattccga
     2101 tttctaaatg aagatcagca aatctttctg ttaccacttt atcggattct tctgtcttta
     2161 ctccctcagg aatcatcggg atatctgaaa ctaataacaa tgcacctctc ggtatttcat
     2221 taaccaggcc aacgataaac aacgtagctg tttccatatc gatagcaatt gtagaaagtt
     2281 ttttaagata ttgtttaaaa tcttcatccc attcccaaac tctgcgattg gtcgtgtaaa
     2341 caactcccgt tctatattcc attccgtgat caataatttt atctgaaaca aatttgtgaa
     2401 gtttaaatga tggcaaagca ggaacttcac ttggtttgta atcatcactc gttccctctc
     2461 cgcgtattgc agcagttggt aaaataaaat gcccgatttc tgtggagcgt tttaatccgc
     2521 cgcatttacc gagaaacaaa actcctttgg gtgcacgtgc tactagtaaa tccattattg
     2581 ttgctgcgtt agcagaacca ataccaaaat ttattattga taatccattt gagttggttg
     2641 ctgtttgcat tggtctgccg atgcctttta cttcgcagtt aaatctttca gcaaatttat
     2701 caacatagtt ttgaaagttt gtgagcagaa aataatctcc aaaatcatct atattagttc
     2761 ctgtgtaacg aggcagccag ttttttgcaa tatcaagctt tgttttcata gcagaattta
     2821 ttgtttgtaa attacatgac gaaattaaat aaatattcaa caacttataa tctgtaaatt
     2881 caataaaatg aactttaaag aagcaataaa ccattttcct cacatcataa ctgagggttc
     2941 gataattgaa agattaaaac gtgaattcca gtatccgctt gataatttgt tatcaaacgc
     3001 gaaaatgatt tatgaagata atgggagagt acttctagaa aatatttatc gtgagtatat
     3061 aaatattgct gaatcatcag atttgccaat tatgttatta actcctacct ggcggacaaa
     3121 taaagaaaga acaaaaaacg ctaacgttga tatgaagatt atcaacactg atgctttttt
     3181 atttgttgag aatattagaa aatccttcgg aagatttgct gagaaaatat ttattggcgg
     3241 tttaactggc tgcaaaggcg atgcatacaa acctgaagaa gcattaaatc aaaacgaagc
     3301 atatcatttt cataaagagc aaatgcagat tcttgcagat gccggagttg actttctttt
     3361 tgcttctact cttcctgctt taacagaagc aattggaatt gcaaaagcaa tgagtgaaac
     3421 caaaaaggat tatgtgataa gttttgtaat tagagaaaat gggaaactac ttgaaggcac
     3481 tctgctaact gatgcgatca aaataattga cgattcggtt ttaactccgc cattatttta
     3541 tttaacaaat tgtattcacc cggatgtact tcataaatca ttttttaatc ttaaagatga
     3601 a
//
LOCUS       DASWBY010000465        10222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_245114, whole genome shotgun sequence.
ACCESSION   DASWBY010000465 DASWBY010000000
VERSION     DASWBY010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245114"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VF870_08300"
     CDS             complement(<1..796)
                     /locus_tag="VF870_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252229.1"
                     /translation="MRTKLLLVSIFLAITVYPQVNTIEQIIDKGIFHKKIINTRDTLS
                     INVLRIDLSTNEYKLGTLKADNLLNAKETTSEMVSTLTDSGYNVIAAINADFFEEGGE
                     IINNMISEGEYVKAVKFTDSPFNPFVNTQFAIMNYNKLLMEQFVFNAQLVLSNGIAEP
                     ISRINSKADSSSITLYNSFQGVCTPKAPDNWFVTEISLNILHKNLDTLFCMVADSFEH
                     GGNKNITTDFVLSTNNKYAHYIEREMNTGDTVKVILQMDPGYSNIIE"
     gene            937..2820
                     /locus_tag="VF870_08305"
     CDS             937..2820
                     /locus_tag="VF870_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9252230.1"
                     /translation="MAINVTPVILSALELEVHFGEQIILDKASLSVHEGDRIGLVGRN
                     GAGKSTFLKIISGILPPDSGEVAKKKDLVVGFLSQEFTLDDYKSVYENILDGASLELN
                     LLREYENTPFDAPNKHHLEEQILRLDSWNLDKRINMLIDSLDAPAADRDIKTLSGGEK
                     RRVALCRALISKPDLLILDEPTNHLDTKSIEWLEVFLAQYNGTCIFVTHDRYFLDRIA
                     NRIVELSSGTFYSHQGNYTEYLINKAERQAIKEVEERKRQMFLRRELEWVKRGPKARR
                     TKAKSRLDNYNEVASQNDYEVELDVELIIPPADRLGKKVLEFKDAGVKLGDRILFQNF
                     NFIFEPGRKIGVVGQNGVGKTTLLKMILGSIPITMGKIEVGETTQFNYVDQARLLLND
                     DDTVIKAIGDGSETVRFGKQQLSIWTYLRRFLFTDDRINTLVGRLSGGEKSRLTLAKI
                     LCNGGNFLMLDEPTNDLDLPTLRILEEALLAFEGCVVVVSHDRYFLNRICNGILAFEG
                     NQEIYYSEGGYDFYIEKKKKREQDLRETKTKEFSEDTRIKNRPKKLSYKDAMELAQIE
                     EKILQAESEVEEIEMIYSSPDFFEKYAAQTSELNLKLEAAKEKVKKLYTRWEELESIK
                     NNL"
     gene            complement(2930..4756)
                     /gene="dsbD"
                     /locus_tag="VF870_08310"
     CDS             complement(2930..4756)
                     /gene="dsbD"
                     /locus_tag="VF870_08310"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEX9252231.1"
                     /translation="MTLISKKIFGSLLFVSMIFGMQLFAQNQDVVNINVVEQSIKVIQ
                     GESVSVNLSIIVNPTWHINSNKPNDDFLIPSEITAKGSGVKLTSIKYPQAKNLKLAFS
                     EEPVSVYEETSNAKLVFLTNSNATVGKQKVLVTLDYQACNNVSCMPPNSTTTEFELEI
                     VKKNDDDKLQTEVLPQSDTSNKEIKSDKLAEENPKVENTKTVISKTEKSDDSSIASTL
                     ESSGLFLSLIFVFLAGLALNLTPCVYPLIPITIGYFGGQAEGKTSRLVMLGLLYMLGM
                     ALTYSVVGVVTSLSGAVFGSLLQNPIVIIIIVLLFVALALSQFGVYEFKLPDSWVAKA
                     GGAKGGAFGAFFMGLTMGIVAAPCIGPFVLGLVTYVAAKGDPLYGFLMFFVLALGLGF
                     PYLLLAIFSGKIKNLPRAGLWMEGVKHIFGFLLIGMAFYFLDPILPKPIQGFTLPIFG
                     IIAAIILLFVDKTANDAKGFRMFKTIFSVIILAVSVYALIPKEKLEPEWKKFSIENYE
                     ASLSNNERMVIDFYADWCIPCKELDALTFSDPRVLKEFDSFTNYKVDMTKNNETNEQL
                     RKRFNVVGMPTVLIIDSEGKEIKRITGFVNADEFLTYIENIK"
     gene            complement(4849..7689)
                     /locus_tag="VF870_08315"
     CDS             complement(4849..7689)
                     /locus_tag="VF870_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9252232.1"
                     /translation="MRPNLILKSLLLLALFCFTIINAQTDFKLDYEKYTLENGLQVIL
                     HQDKSDPITAVSIQYHVGSNRETPGKTGFAHLFEHIMFQESQHVPQDQFFKLIQGNGG
                     SLNGGTWQDGTMYFEIVPNSALELALWLESDRMGFMLPTVTYDAFLNQQNVVQNEKRQ
                     NYNNRPYGQTSYLIGKLLYPDGHPYNWTTIGSLEDLQNATLKDVHDFYTKWYGPNNAT
                     LVVAGDFDVDQTKKWIEKYFGEIKPSAPVSPLKPQLVKLDDYKRASYEDNFAQSPELT
                     MLFPTIEQYTKDSYALDVLADLIGIGKKSPLYKVVVEDKKLAPSVVTYNNSSEVAGDF
                     RIRIRTFPDKNLNEVEKAIKESFAKFEKDSFTEEDLARTKAGIETNFYNGISSVLGKS
                     RQLASYNVFTGSPDFLNTDLNNSLAVTSEDVWRVYNKYIKDQKYVLLSIVPKGKSNLA
                     AENSPPFPITGDNIKTYTDEEKTQITNLPENLVKVEKIVSKFDRSKQPVNGPDPLLKE
                     QKIWDDNLSNGIKVLGIEQKELPLVDFQIAIKGGLLLDDINKVGAANLVSSMLMQGTK
                     NKTPLELEQAIEDLGSNINVYATDDAVLIRANCLSSKFAETFRLVEEILFEPRWDVKE
                     FERLKNETIESLRRSKTSASSTASLVFSKLIYGDDNILSKNTAGTEESVSAITLDDLK
                     NYYAKSLSPQNSNITIAGDINKDEAINVFKDLESKWNKIDVLIPQPKFANDIQTSKVY
                     FIDFPGAKQSEIRIGSMGLAFTDPDYFKVTVMNYKLGGSFNGIVNLILREEKGYTYGA
                     RTIFTGSEFPGYFVASSGVQSDATLESAEIFKDEMTKYRNGLSPEDLIFTKDALIKSN
                     ALRFETIGALRGMLSQIAWYNLPYDYVKDQEKQITDMTIEQHKMLAQKYIHPDKMIYL
                     IVGDKATQFEKLKGLGFGDPILLDKDGKIISN"
     gene            7893..8429
                     /locus_tag="VF870_08320"
     CDS             7893..8429
                     /locus_tag="VF870_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252233.1"
                     /translation="MKTLHLLIILSIVCTFPPILFSQDEADNQEHRIVMEDPSQYLPD
                     YLDNIYIGMPLADFENVKDTLTMDLSDNESALWYGIRETVNDQGISEITYKFDKEEDG
                     VNAENPLYQINIVFLEPDYQNDYLQQKFNIAPKDLEPTNKEWEFSTDKNYLLIVRQND
                     NEVMIIATMPGTEWDSNN"
     gene            8517..9503
                     /locus_tag="VF870_08325"
     CDS             8517..9503
                     /locus_tag="VF870_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9252234.1"
                     /translation="MKEINSPEGIRDLANAFRESRVLLSAFELKIFTVLDKHLMTSEE
                     VSAKINTETRATDRLMNALCGMGLLRKVKGKFYNTDLSSKYLVEGKPEFMGNLYHANH
                     LWSTWSYLTESVKQGGSFKGDQNKKEKDEWVEAFIGAMHYRGVNQGKVLSMMIDLSNV
                     KKMLDVGGGSAAFSMEIVKKNSAIKAAVLDLPHVIPLTKKYVGEAGLLGNFNFIEGDY
                     LTKDFEDDYDLILLSAIVHINSYDQNKMLIKKCADALNKNGMIIISDFVMNDDRTQPF
                     HGALFSINMLVGTANGDTYTEREMRKWYESAGLSKIERKNTAFGSDLMIGIK"
     gene            complement(9624..>10222)
                     /locus_tag="VF870_08330"
     CDS             complement(9624..>10222)
                     /locus_tag="VF870_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9252235.1"
                     /translation="PIEDTVGAMSDLVKEGKIKYLGLSEAAPDTIRRGNKVHKISALQ
                     TEYSLWTRDSEKELLNLCKELEITFVAYSPLGRGFLSGRFKPGQELPDNDYRRTHPRF
                     QEENISHNFKLIERLDEVAKEKNCTTAQLALAWVLHQRDFIVPIPGTKNIKYLEENIA
                     ALDLKFSESELKEIEKIAPIGFTKGMRYAEQAMKAVNR"
BASE COUNT         3399 a         1707 c         1702 g         3414 t
ORIGIN      
        1 gttcaataat atttgaataa cctggatcca tctgtaaaat gactttaacc gtatcacctg
       61 tattcatttc tcgttcaatg taatgagcat atttattatt tgtcgataac acaaagtctg
      121 tagttatatt tttattcccg ccatgttcaa aggagtctgc aaccatgcag aacagtgtat
      181 ccagattttt atgtaaaata ttcaaagata tttcagttac aaaccaatta tccggtgctt
      241 tgggtgtgca caccccctgg aaagaattgt aaagtgtaat tgaactgcta tctgcttttg
      301 aattaattct acttattggt tctgcgattc cgttagataa aactaactgc gcgttaaaaa
      361 caaattgttc cataagtaat ttgttataat tcataattgc aaattgagta ttaacaaatg
      421 gattaaaagg cgaatctgtg aacttaacag ctttaacata ctccccttct gaaatcatat
      481 tattaattat ttcaccacct tcttcaaaaa aatctgcatt aattgctgct ataacgttat
      541 agccggaatc tgttaatgtg cttaccattt cgctggtcgt ttcttttgca ttaagaagat
      601 tgtctgcttt aagtgttcct aacttatatt catttgttga tagatcaatt cttagtacat
      661 ttattgaaag agtatctcgg gtgttgataa tctttttgtg aaaaattcct ttatcaataa
      721 tttgttcaat tgtgtttact tgtggataga ctgttattgc taagaatatc gaaacaagta
      781 gaagttttgt tctcataata gcctcctgat tgttattaaa gaattttcaa cttaaaatta
      841 agtaatatct ggttacgatg ttattgggca aatttattaa agtggctgat gtatatttgc
      901 aggcaataaa aatattaatt aacaattatt tcttaaatgg ctataaacgt tacgcctgta
      961 attctatcag cacttgaact tgaagttcat tttggtgagc agataatcct tgataaagct
     1021 tcgttaagtg tgcatgaagg tgaccgcatt ggattggtcg gaagaaatgg cgcaggcaaa
     1081 tcaactttct taaaaatcat ttcaggtatt cttcctcccg attccggtga agttgcaaag
     1141 aaaaaggatc tagtcgttgg atttctttca caggaattta cattagacga ttataaaagt
     1201 gtgtatgaaa atatccttga tggcgcttct ctagaattaa atctcttaag ggaatatgaa
     1261 aacactccgt ttgatgctcc aaataaacat cacttagaag aacaaattct tagacttgat
     1321 tcgtggaatc ttgataaaag aattaacatg ctgatcgatt cattagatgc tcccgctgcg
     1381 gatcgggata ttaaaacact ttcgggcgga gaaaaaagac gggtagcgct ttgcagagca
     1441 ttaatatcaa aaccagattt actgattctg gatgaaccga caaaccacct tgatacaaaa
     1501 tcaatagaat ggcttgaggt ttttctggcc cagtataatg gcacctgtat ttttgtaaca
     1561 cacgatagat attttcttga tagaattgca aacagaattg ttgaactttc atcaggtaca
     1621 ttttattccc atcaagggaa ttatacagaa tatttaatta ataaagctga acgccaggca
     1681 attaaagaag ttgaagaacg taagcggcaa atgtttttac gacgtgaact tgaatgggta
     1741 aaaagaggac ccaaagcgcg ccgaactaaa gccaaaagca gacttgataa ttataatgaa
     1801 gttgcatcac aaaatgatta cgaagttgag cttgatgttg aacttataat ccctcctgct
     1861 gatagacttg gtaaaaaagt cttggagttt aaagatgccg gtgtaaaact tggtgataga
     1921 atattattcc aaaattttaa ttttattttt gaacctggaa gaaaaatcgg agttgttgga
     1981 caaaacggag taggcaaaac tactttacta aagatgattc ttggttcaat tccaataact
     2041 atgggaaaaa tagaagttgg tgagacaaca cagtttaatt atgtagatca ggcaagatta
     2101 ttattaaatg atgatgatac tgttataaaa gctatcggtg atggaagtga aacggttaga
     2161 tttggtaaac agcaacttag tatctggact tatttacgca gatttctatt tacggatgat
     2221 cgaataaata ctcttgtcgg aaggttatcc ggtggagaaa aaagccgact aacacttgca
     2281 aagattttgt gtaacggcgg caattttctt atgctggatg aacctacaaa cgatcttgat
     2341 ctgccaactt taagaattct tgaagaagca cttttagcat ttgagggatg tgtggttgtt
     2401 gttagtcacg atagatattt tctaaatcgt atttgtaatg gcattctggc atttgaggga
     2461 aatcaggaga tttattacag cgaaggcgga tatgattttt atatcgaaaa gaaaaagaaa
     2521 cgtgaacagg atctgcgcga aacaaaaaca aaagagttta gcgaagatac aagaataaaa
     2581 aacagaccaa agaagcttag ttataaagac gcaatggaac ttgctcagat tgaagaaaaa
     2641 attctgcagg cggaatctga ggttgaagaa attgaaatga tttatagttc tccggatttt
     2701 tttgaaaagt atgcagcaca gacaagtgaa ttaaatctaa agcttgaagc agcaaaagaa
     2761 aaagttaaaa aactctatac ccgctgggaa gaactggaat caataaaaaa taatttatag
     2821 ataactaata atttattttc aatgagtacc ttataaacct aaaaaaatat tgtcaccctt
     2881 agcaaagtcg aagggtgaca caacatcaaa ttataatggg aaatttcagt tattttatat
     2941 tttcaatata agttaaaaac tcatctgcat taacaaaacc ggttatcctt ttaatttcct
     3001 ttccttcaga atcgataata agtacagtcg gcattccaac tacattaaat cgttttctaa
     3061 gttgttcatt ggtttcatta ttctttgtca tatcaacttt ataatttgta aagctatcaa
     3121 attcttttaa cactcgcgga tcagaaaatg ttaatgcatc taattcttta caaggaatac
     3181 accagtcagc ataaaaatct attaccattc tttcattatt gcttaatgaa gcttcataat
     3241 tttcaatact gaatttcttc cattcaggtt caagtttttc ttttggaatt aatgcatata
     3301 ccgaaacggc tagaattatg actgaaaaaa ttgttttgaa cattctaaat ccttttgcat
     3361 catttgcagt tttatcaaca aaaagtaaaa tgatagctgc aattattcca aatattggaa
     3421 gtgtaaaacc ttgaataggt ttaggtagaa tcggatctaa aaagtaaaat gccattccaa
     3481 ttaataagaa accaaaaata tgttttactc cttccatcca taaacctgca cgtggtaagt
     3541 tttttatttt accggagaat attgcaagca gcaaatatgg aaatcctaaa cctagtgcca
     3601 ggacaaaaaa catcagaaat ccataaagtg gatcgccttt ggcagcaaca tatgttacaa
     3661 gaccaagcac aaaaggtcca atacaaggtg cagcaacaat acccattgtt aatcccataa
     3721 agaatgcacc gaatgcaccg ccttttgctc cacctgcttt tgcaacccag gaatccggaa
     3781 gtttaaattc gtaaactcca aactgactta aagctaaagc aacaaatagc aggacaataa
     3841 ttatgataac aattgggttt tgtaatagac taccaaacac cgcgccgctt aatgatgtta
     3901 caacacccac aacggaataa gtcagcgcca tcccaagcat gtaaagtaag ccaagcatta
     3961 ctaaacgact ggtcttccct tctgcctgtc cgccaaagta accaattgta ataggtatca
     4021 aaggataaac acaaggagtg agatttagtg ctaatccagc aagaaaaaca aaaattaaac
     4081 ttaaaaacag tccgctgctt tccaatgttg atgcgattga actgtcatca cttttttctg
     4141 ttttggaaat tactgtttta gtattctcta ctttaggatt ttcttccgct aatttatcag
     4201 atttgatttc tttattactt gtatctgatt gaggaagaac ttcggtttgt aatttatcat
     4261 catcattctt ttttacaatt tctaattcaa attctgttgt agtggaattt ggcggcatac
     4321 aggaaacatt attacaagcc tgataatcca aggtaactaa aactttttgc tttccaactg
     4381 ttgcgtttga atttgttaaa aaaacaagtt tagcatttga agtttcttca taaactgaaa
     4441 cgggttcttc agaaaaagct agttttaagt tttttgcctg tggatatttt atacttgtaa
     4501 gttttactcc actgccctta gcagttattt cacttggtat caggaaatca tcattaggtt
     4561 tatttgaatt tatatgccag gttggattaa cgatgatgga taaatttaca gatacacttt
     4621 ctccctggat tacttttatt gattgttcaa ctacattaat atttacaaca tcctgatttt
     4681 gagcaaataa ttgcatccca aagatcattg aaacaaataa taaagaacca aatatctttt
     4741 tacttattaa agtcatttgt ataccatttc tttttactta aaaataactt gcaaaatgaa
     4801 tcatttataa ggtctatttg atgataatta aattgaaaaa aattcatatc aattactaat
     4861 tatttttcca tccttgtcta ataaaatagg atccccaaat cctaatcctt ttaatttttc
     4921 aaactgcgta gctttatctc caacaatcag ataaatcatt ttatccggat ggatatactt
     4981 ttgagcaagc attttgtgct gttcaattgt catatcagta atttgttttt cctgatcttt
     5041 tacataatca taaggaagat tgtaccaggc aatttgagat aacattccac gcaaagcacc
     5101 gattgtttca aatctaagtg cattggattt aattaatgcg tcttttgtaa atattaaatc
     5161 ttccggcgat aatccgtttc tgtactttgt catctcatct ttaaatattt cagcggattc
     5221 taaagtagcg tctgattgaa cacctgaaga tgcaacaaag taaccaggaa attctgatcc
     5281 ggtaaaaatt gttcttgcac cgtacgtgta tcctttttct tctcttagaa taagatttac
     5341 aattccgtta aaacttccgc caagtttata attcataaca gtaactttaa agtaatccgg
     5401 atctgtaaaa gctaatccca tagatcctat tcttatttct gattgctttg cacccgggaa
     5461 atctataaag taaacttttg atgtctgtat atcattagca aattttggtt gtggaattaa
     5521 aacatcaatt ttattccatt tactttctag atctttaaat acattaattg cttcatcttt
     5581 atttatgtca cctgcaatag taatattaga attttgtggt gaaagacttt ttgcgtaata
     5641 gtttttaaga tcatctaaag ttatagcaga aactgattcc tctgtacctg cagtattttt
     5701 agaaagaata ttatcatcac cataaattag tttagagaaa actaatgatg cggtagaact
     5761 tgcagaagtt ttggatcttc ttaaactttc tatcgtttca tttttaagtc tttcaaattc
     5821 ttttacatcc cagcgcggtt caaataaaat ttcttcaaca agtctaaatg tttcagcaaa
     5881 ttttgatgac aaacaatttg cgcgtattaa aacagcatca tcggtagcat aaacattaat
     5941 gttagaacca agatcttcaa tagcttgttc taattccagg ggagttttgt tctttgtacc
     6001 ctgcatcaac attgagctta caagatttgc agcgcctacc ttatttatat catcaagcag
     6061 caaaccgcct ttaattgcga tttggaaatc aactaatggt aattctttct gctcaatgcc
     6121 aagcacttta attccatttg ataaattatc atcccaaatc ttctgttctt taagaagtgg
     6181 atccggtcca ttaaccggct gttttgatcg atcaaatttt gaaacaatct tttcaacttt
     6241 tactaaattc tcaggcagat tcgttatttg agttttttct tcatctgtat atgttttaat
     6301 attatcgcca gttattggaa atggaggaga attttcggca gcaagatttg atttaccttt
     6361 agggacaata cttaacagaa catatttttg atccttaatg tacttgttat aaacgcgcca
     6421 cacatcttct gatgttactg caaggctatt attcaaatct gtgttaagaa aatccggtga
     6481 gccggtaaaa acattatagc tggcaagttg tcttgattta ccaagaacgc tcgatattcc
     6541 attataaaag ttagtttcta ttccagcttt agttctggca agatcttctt cagtaaaaga
     6601 atctttttcg aattttgcaa atgattcttt aattgccttc tcgacttcat tcaagttttt
     6661 atcaggaaaa gttcttatgc ggattctgaa atcacctgct acttcgctgc tgttattgta
     6721 agtcacaaca gaaggagcta gttttttatc ttcaacaact actttataca aaggagattt
     6781 cttccctatt ccaatcaaat ctgctaacac atctaatgca taactgtctt tagtatattg
     6841 ttcaatcgtt ggaaacaaca tagtcagttc gggtgattgt gcaaagttat cttcatatga
     6901 agcacgctta tagtcatcca attttacaag ttgaggtttt aaaggtgaca caggtgcaga
     6961 cggttttatt tcaccaaaat atttttcaat ccattttttt gtttggtcaa catcaaaatc
     7021 gccggcaacc acaagcgtag cattatttgg tccataccat tttgtgtaga aatcatgcac
     7081 atctttaaga gtagcatttt gcaagtcttc aagagaacca atagttgtcc agttgtatgg
     7141 atgtccatcc ggatataata atttacctat aagatagctc gtctgtccgt aaggccggtt
     7201 attataattt tgtcttttct cattctgcac aacattctgc tgatttagaa aagcatcata
     7261 tgtaactgtc ggaagcataa atcccattct gtcagattca agccaaagag ctaactcaag
     7321 tgcactgtta ggaacaattt caaaatacat tgtaccatct tgccaggtgc cgccatttaa
     7381 agatcccccg ttgccctgaa taagcttaaa aaactgatcc tgtggaacgt gttgtgactc
     7441 ctgaaacata atgtgttcaa acaaatgagc aaaacctgtt ttacccggtg tttccctgtt
     7501 agaaccaaca tgatattgaa ttgaaactgc agtaatcgga tcggatttat cctgatgcaa
     7561 tattacctga agtccgtttt ctaaagtgta tttctcataa tcaagtttaa aatcagtctg
     7621 agcattaatt attgtaaaac aaaacaatgc taaaagtaaa agcgatttca aaattagatt
     7681 cggtctcatt aataatctct cctgtaaatt attttaaata attgtaaaac aaatatacaa
     7741 cactctgaaa ataatttagc atactttttt agtgtcatgt gccgcttcgg tagtttaact
     7801 tatatgaata taatttacag taaagattta tacttaataa tttgttaaag tactttttga
     7861 tattaaattt aaataaacag attggagttt ttatgaaaac acttcactta ttaataattc
     7921 tttctattgt atgtactttt cccccaatat tattttctca ggatgaagca gataaccagg
     7981 aacatcgaat cgttatggaa gacccatctc agtatcttcc ggattatctg gacaatatat
     8041 acataggtat gccgcttgca gattttgaaa atgtaaaaga tacattgact atggatttga
     8101 gcgataatga atctgctctc tggtatggta ttcgagaaac tgtgaacgac cagggcatta
     8161 gtgagattac atataaattt gataaagaag aggatggagt taatgctgaa aatcctttat
     8221 atcaaatcaa cattgttttt cttgaaccag attatcagaa tgattatttg cagcaaaagt
     8281 ttaatatagc tcctaaagat ttagagccga caaataaaga atgggaattt agcacggata
     8341 aaaattatct cttgatagtc aggcaaaatg ataatgaagt tatgataatt gcaactatgc
     8401 ctggcactga gtgggattca aataattgat cgaggtatta ttaaaagaat gttttttcgt
     8461 taagcagact tagaccttaa gagcactctg taacgaataa ttaaactgga attaaaatga
     8521 aagaaattaa ttctcccgaa ggtataagag acctggcaaa tgctttccgt gaaagccgcg
     8581 tattattgtc ggcatttgaa cttaagatct tcactgtgtt ggataaacac ttaatgactt
     8641 cagaggaagt ctcagcaaaa ataaataccg aaaccagagc aacggacaga cttatgaatg
     8701 ccctctgtgg aatgggattg cttagaaaag ttaaaggtaa attttataac actgatttat
     8761 cttcaaaata ccttgtcgaa ggtaaacctg aatttatggg aaatctttat catgccaatc
     8821 atctctggag tacctggagt tatcttacag agtcagtaaa acaaggcggt tcttttaaag
     8881 gcgatcagaa taaaaaagaa aaagatgaat gggttgaagc atttattggt gcaatgcatt
     8941 accgtggtgt taatcagggt aaagttcttt cgatgatgat cgatctttca aatgtaaaaa
     9001 agatgttaga tgtcggcgga ggttctgcag ctttttcaat ggaaatagtt aaaaagaatt
     9061 cagccattaa agctgctgtt ttggatttac ctcacgtaat tccacttaca aaaaagtacg
     9121 ttggcgaagc ggggctactg ggtaatttta attttattga aggagattat ttaactaaag
     9181 attttgaaga tgattacgat ttaattttat tatctgccat tgttcacatc aacagctatg
     9241 atcaaaataa aatgcttatt aaaaaatgtg cagatgcact gaataaaaac ggtatgatca
     9301 taataagtga ttttgtaatg aatgatgaca gaacacagcc atttcatggt gctttgtttt
     9361 caattaatat gcttgtcgga actgctaacg gtgatactta tacggaacgg gaaatgagaa
     9421 aatggtatga atcagcaggt ctttctaaaa ttgaaagaaa aaatacagct ttcggttcag
     9481 atttgatgat tggaattaaa taactataca aaatatttta ggtgcgaata atgccaatca
     9541 gtttgaaaat aaattctcaa aagacagcga taaaagtctt tttataatta atctaagatt
     9601 caaagtggac aataaatttt aaattatctg tttacagcct tcattgcctg ttcagcgtat
     9661 ctcatacctt ttgtaaatcc tatcggagct atcttttcaa tctcctttaa ttcactttca
     9721 gaaaacttca gatcgagagc agcaatattt tcttcaagat attttatatt ctttgtccct
     9781 ggaatcggaa caataaaatc tctttgatgt aagacccaag ccaaagctaa ttgagccgta
     9841 gtgcagtttt tctcttttgc tacctcatca agcctttcaa ttaatttaaa gttatgtgaa
     9901 atattttctt cctgaaaacg tggatgagtt ctacgataat cattatccgg taattcttgt
     9961 ccgggcttaa atctacccga taagaatcct ctacccaacg gagaatatgc aacaaaagta
    10021 atttcaagtt ctttacataa attcaaaagt tctttttcgg aatcccttgt ccacaaagaa
    10081 tattctgttt gaagtgccga gatcttgtga actttatttc cacgtctgat agtatccgga
    10141 gcagcttccg ataatcccaa atatttaatc tttccttctt ttactaaatc agacattgca
    10201 cctacggtat cctcaattgg aa
//
LOCUS       DASWBY010000466         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_544783, whole genome shotgun sequence.
ACCESSION   DASWBY010000466 DASWBY010000000
VERSION     DASWBY010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544783"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VF870_08335"
     CDS             <1..255
                     /locus_tag="VF870_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252236.1"
                     /translation="FPVELVVLGGDIFSNNPYPFDIKPLITYILIGIEFVVILWSFYL
                     IYRSIVTVNLSKIYSLFLTTAFLIVWFVTLYFSSKIIFTI"
     gene            323..>1715
                     /gene="nadB"
                     /locus_tag="VF870_08340"
     CDS             323..>1715
                     /gene="nadB"
                     /locus_tag="VF870_08340"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX9252237.1"
                     /translation="MSLKTDVLIIGSGIAGLFAALKISEFADVILVTKKNKAESNTNY
                     AQGGIASVIDPNDSFEKHVEDTLIAGAGLCDRKAVESMVKEGPERINDLIQIGTMFTK
                     KGNDFDLAREGGHSMSRILHAKDLTGKEIERALIHSVEEKSNIKIYENAIAIDLLTEH
                     NLSRRPETLNKNKNCWGAYILDSTKNEVLKIKAKATVLATGGLGQVYLHTTNPLIATG
                     DGFAMGYRAGVEIANMEFIQFHPTSFYSNKSNYNSDGHSFLISEAVRGFGGLLRTKDG
                     NLFMHNYDKRKELAPRDIVARAIDTELKKRGDEFVYLDLTHKDANEIIDHFPNIYSTC
                     LKNDIDITKEFIPVVPAAHYACGGIVVDLNGQTSLEGLFASGEVTMTGVHGANRLASN
                     SLLEALVYAHRCSNSIREFIKNYDVKVPHLLDWNYSGKLTYDERVLITHSVDEVKQTM
                     WDYVGIVRSDLRLE"
BASE COUNT          591 a          250 c          324 g          550 t
ORIGIN      
        1 tttcctgtgg aattagttgt attgggaggc gatatatttt ctaacaaccc atatccattt
       61 gatataaaac cattaattac ttacatctta atcgggattg aatttgtagt aattctttgg
      121 tctttttatt tgatttatag aagtattgtt actgtaaatt tgagtaaaat ttattctctc
      181 ttcttaacta ccgcattttt aattgtatgg tttgttactc tctatttttc ttcaaaaata
      241 atatttacta tttaatactg tttttaatta tccttaatta agtattttaa ctattatttc
      301 atcaaaaata atgggaatta aaatgtcact caaaacagat gtacttatca tcggaagtgg
      361 aattgcaggg ttatttgctg cacttaagat ttcagaattt gcagatgtaa ttcttgtaac
      421 aaaaaaaaat aaagcagagt caaatactaa ttatgctcaa ggtggaattg catccgttat
      481 tgatcctaat gattcctttg aaaaacatgt tgaagatact ttaattgctg gcgctggtct
      541 atgtgataga aaagcagttg aatcaatggt gaaggagggt cctgaaagaa taaatgatct
      601 aattcagata ggaacaatgt tcacaaaaaa aggaaatgac tttgatttag caagggaagg
      661 cggacactca atgtcaagaa ttcttcatgc caaagattta acaggaaagg agattgaaag
      721 ggcattaatt cattcagtgg aagaaaaatc gaatattaaa atctatgaaa atgcaattgc
      781 aattgatctt ttaacggagc ataatttatc ccgcaggccc gaaacactca ataaaaataa
      841 aaactgctgg ggagcttata ttttagattc aacaaaaaat gaggtactaa aaattaaagc
      901 taaggctact gtattagcaa cgggcggttt gggacaagta taccttcata ctacgaatcc
      961 attaattgcc acaggtgatg gatttgcaat gggttatcgg gcaggagttg agattgcgaa
     1021 tatggaattt attcaatttc acccaacttc attttacagt aataaatcta attataactc
     1081 agatggccat tcatttctaa tatcagaagc agtccgggga tttggaggat tgctccgtac
     1141 aaaagatggc aatcttttta tgcacaatta tgataaaaga aaagaattag caccgagaga
     1201 tattgtagca cgtgcaattg atactgaatt aaaaaagcgc ggcgatgaat ttgtttactt
     1261 agatttaact cataaggatg ctaacgaaat aatagatcac tttccaaata tctattcaac
     1321 ctgtttaaaa aatgatattg acattactaa agaatttata ccagtagttc cggcggctca
     1381 ctatgcatgc ggcggaatag ttgttgattt aaatggacaa acttccctcg aagggttatt
     1441 tgcaagcggg gaagtaacaa tgacaggagt gcatggtgca aacagactcg ctagtaattc
     1501 gcttttagag gcacttgtgt acgcgcatag gtgctcaaat tctattagag aatttattaa
     1561 aaattacgat gttaaggttc ctcatttact agactggaat tactcgggta aacttaccta
     1621 tgatgaaagg gttctaatca ctcatagtgt tgatgaagta aagcaaacaa tgtgggatta
     1681 tgttggaatt gtcagatcag atttaagact tgaac
//
LOCUS       DASWBY010000467         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_169203, whole genome shotgun sequence.
ACCESSION   DASWBY010000467 DASWBY010000000
VERSION     DASWBY010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169203"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3132
                     /locus_tag="VF870_08345"
     CDS             <1..>3132
                     /locus_tag="VF870_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9252238.1"
                     /translation="GQHKKSTNKRIPFENFLVDSNGEEIPIEVLASNLTLNGKAVIQG
                     VFRNISERKKIEAALKESEHKFQLFFEYSKDAMLLLDGEVFFDCNNAAVEMLGYKNKS
                     QMLSVHPAKLSPEYQPDGVSSYTKAERIIKQAYKDGFVKFEWMHRKKSGEDFPIEVML
                     TSIPLEGKQILFTVWRDITERKNYELEIQKSKDRMRLLVEGTPHLFFYVQNNEGTIEY
                     ISPSIENITGHSASEWLNQNNWFITDSPINNIAKERTKEHLSGKVNTDPIYVEILHSN
                     GSNVTIEIYERPIFLNGKVIGLQGVAHDITQRLQFEKSLKESEESYRGLFNSVTESIY
                     IQDENGVFLDVNEGASLMYGYDRETLIGKTPDFVSAPGKNDLDAIGLMSRKAFLGEKQ
                     QFEFWGKRKNGEEFLKDVRLYPGTYFNNKVIIAIATDITEKKKVEQLLLESEERYRAI
                     SNLTSDYLFSTKADENGEHHIVWIAGSFEKITGYTIDEYHKIGGWRATLHPDELELDS
                     LDLEKLRRNEKVNRQIRVYHKNGSLIWVRSSATPVWDKRTNRLIGIYGAVEDVTQSKQ
                     SEIIQKIQYNIADAAVTYKTLTELFEIIRLELSAIINVNNFFIALYDEKTGMLKSDVE
                     RDEMEEIAEWPANGSMTGYVIEQKKSALLKKDEINHLIKNGIAGMVGTIPEVWLGVPF
                     KIGGKVFGVLVVQSYDNPNAYDKRSIEILEIVAHELSLFINHKKAEEETLKLSTVVVQ
                     SPIAVVITDPKGNIEYVNPKFSEVTGYSFEEVKWNNPRILNSGLQSQEFYSNLWNTIL
                     AGKTWQGEFRNKKKNGEVYWENALISPIIDADGRILYFVAIKEDISEKKKMIQELIKA
                     KEDAEEMNRIKSSFFANMSHELRTPMMGILGFSEVLMQELKDYPDYLRMVNSINSSGQ
                     RLLETLNLILNLSKLEASKVNFELNTQNIIPVLKECFESFESAAAKKNIHYTFISEYP
                     EIMCNIDKLLFTSIFNNLLNNAIKFTDSGNVTLSASIDSDFAVVSITDTGVGISEAKQ
                     NLIWEEFRQASEGY"
BASE COUNT         1151 a          454 c          626 g          901 t
ORIGIN      
        1 ggccagcata aaaaatcgac taacaaaaga ataccttttg aaaattttct ggttgattca
       61 aacggtgaag aaatccccat tgaggtttta gcaagtaacc ttacattaaa tgggaaagcg
      121 gtaattcagg gagttttcag aaatataagt gagagaaaaa aaatagaagc agcacttaaa
      181 gaaagtgaac ataagtttca attgttcttt gaatattcta aagatgcaat gctactgctg
      241 gatggcgaag tattttttga ttgtaataat gcagcagttg aaatgctggg atataaaaat
      301 aaatctcaaa tgctttctgt gcatccggca aaactctctc cggagtacca gccggatgga
      361 gtatcatcct acacaaaagc agaaagaata attaagcagg cgtataaaga tggatttgtt
      421 aaatttgaat ggatgcatcg aaaaaaaagc ggtgaagatt ttccgataga ggttatgctt
      481 acttctattc ctttagaagg taagcagata ttatttactg tttggcggga tattactgaa
      541 cgaaagaatt atgaattaga aatacagaag agtaaagata gaatgcggct gcttgttgaa
      601 ggcacgccgc atcttttctt ctatgtccag aataatgaag gaactatcga atacatttca
      661 ccctctatcg aaaacattac aggtcattct gcttccgaat ggttaaacca aaataattgg
      721 tttattactg actctccaat taataatata gccaaagaaa gaactaaaga gcatctaagc
      781 ggaaaagtaa atactgatcc gatttatgta gaaatattac attctaatgg cagcaatgtt
      841 acaatagaaa tatatgaacg tccaatattt ctaaatggca aagttattgg attacaaggt
      901 gttgctcatg atataacaca aagactacaa ttcgagaaga gtctaaagga aagtgaagaa
      961 agctatcgcg gtctttttaa cagtgtaaca gaatctattt acatacagga tgagaacgga
     1021 gtttttcttg atgttaatga aggagcttca ttaatgtatg gttatgacag agaaactctt
     1081 ataggaaaaa ctcctgattt tgtgagcgcc ccgggtaaaa atgatttaga tgcgattggt
     1141 ttgatgtccc gtaaagcatt tttaggcgag aagcagcaat ttgaattttg gggaaaacgt
     1201 aaaaatggtg aagaattttt gaaggatgtc cgtttatatc ccgggacata ttttaataat
     1261 aaggtaatca ttgcgattgc tactgacata actgaaaaga aaaaagttga acagcttctt
     1321 cttgaaagtg aggaacgata tcgcgctatc tctaatttaa cttcagatta tttgttctca
     1381 acaaaagctg atgaaaacgg agagcatcat attgtttgga ttgcgggctc atttgaaaaa
     1441 ataacggggt acacaattga tgaatatcat aaaattggcg gatggagagc cactttgcat
     1501 ccggatgaac ttgaacttga tagccttgac ctggaaaaat tacgcagaaa tgaaaaagta
     1561 aatcgtcaga tcagagttta tcacaaaaat ggttctctta tttgggttcg atcatcagca
     1621 actcctgtgt gggataaaag aactaataga ttgattggca tctatggcgc agttgaagat
     1681 gtaacccaga gcaaacaatc ggaaataatt cagaagatac aatataatat tgcggatgct
     1741 gcggttactt ataaaacact aaccgagttg tttgaaatta tcagattaga attatcagca
     1801 ataataaatg ttaataattt cttcattgct ctttatgatg aaaaaaccgg aatgctaaag
     1861 tctgatgttg aaagagatga aatggaagaa attgcagaat ggcctgcaaa tggttcgatg
     1921 acaggatatg ttattgaaca gaaaaaatca gcactattaa agaaagatga aataaatcat
     1981 ctaattaaaa acggtatagc agggatggtt gggactattc ctgaagtatg gctgggtgtg
     2041 ccatttaaaa tcggcggaaa agtttttgga gttttggtag tacaaagtta tgacaatcct
     2101 aacgcttacg ataaaaggag tatagaaatt cttgaaatag ttgcgcatga actaagccta
     2161 tttattaatc ataaaaaggc ggaagaagaa accctaaaac tttcaactgt tgttgtgcaa
     2221 agccctatag ctgtggttat tactgaccca aaaggaaata ttgaatacgt gaatccaaaa
     2281 ttttctgaag tgaccggtta ttcatttgaa gaagtaaaat ggaataatcc gagaattcta
     2341 aattcagggt tacagtctca agaattttac tcaaatctct ggaatactat tcttgcaggt
     2401 aaaacttggc aaggagaatt cagaaataaa aagaaaaacg gagaagttta ttgggagaat
     2461 gcacttatat ccccaattat tgacgccgat ggaaggattc tttacttcgt agcaattaaa
     2521 gaagatatca gtgaaaagaa aaagatgata caggaattaa ttaaggcaaa agaagatgct
     2581 gaagaaatga acaggatcaa atcgagtttc tttgcgaaca tgagccatga gttaagaaca
     2641 ccaatgatgg gcatacttgg attcagtgaa gtacttatgc aggaactaaa agattatccg
     2701 gattatttaa gaatggtcaa ttcaattaat tcatcaggac aaagattact tgaaacatta
     2761 aatcttattc taaatttatc caagctagag gcttcaaaag taaattttga acttaatact
     2821 caaaacatta ttccggtttt aaaagagtgc tttgaatcct ttgaatctgc agcagctaaa
     2881 aaaaatatcc actatacatt catctcagaa tatccggaaa ttatgtgcaa tattgataag
     2941 cttcttttta caagtatttt taataatcta ttgaataatg caatcaagtt tacagattct
     3001 ggaaacgtta cattaagtgc atccatagat tctgattttg cagtagtatc aataacagat
     3061 accggagttg gcatttcaga agctaaacaa aatctaattt gggaagaatt ccggcaagcc
     3121 agcgaaggtt at
//
LOCUS       DASWBY010000468         4552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_54686, whole genome shotgun sequence.
ACCESSION   DASWBY010000468 DASWBY010000000
VERSION     DASWBY010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54686"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VF870_08350"
     CDS             complement(<1..1075)
                     /locus_tag="VF870_08350"
                     /inference="COORDINATES: protein motif:HMM:NF044821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252239.1"
                     /translation="MKNIISIIVACIFTISSFAQQQSNWQNYADMKSAKDLAATSTGV
                     WVASQGGAYFFNISDNSFQTYSKASGLNGTELTAIGIDYYGKVWFGSASGLIDVYDPA
                     SNSFRSILDISNSDKTNKRINDITISGDTVYIATDFGISLIDSKNYIFYDTYFKFGSF
                     TSNIKVNNVLFSDLIYAATESGVAIQKAGATNLSAPESWNVYDQSSGLPTGGANVLSF
                     YNSTLIAGTNSGLYSFDGNTWQPFLSLAGNKILDLLVTSNSLNILTPTQVFTYDGTDL
                     SELWNTDSQPVKLSYQENAGLIVATKLGINVNNNFVYPNGPAANQFPNMTVDKEGNLW
                     SASGKDVTGVGIYKYDGSEWETYN"
     gene            complement(1182..1643)
                     /gene="ribE"
                     /locus_tag="VF870_08355"
     CDS             complement(1182..1643)
                     /gene="ribE"
                     /locus_tag="VF870_08355"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9252240.1"
                     /translation="MKSIEGKLIGSGKKFAIIFSRFNEFVGDGLLKAANDCLIKHGVT
                     ENNIDIIKVPGAFEIPVTADRLASKKKYDSIICLGVIIRGATPHFEYVASTTSTGISQ
                     VSQKYSLPVIFGVLTTDTIEQAIERAGTKAGNKGWDSALTALEMADLFKLI"
     gene            complement(1646..2476)
                     /locus_tag="VF870_08360"
     CDS             complement(1646..2476)
                     /locus_tag="VF870_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX9252241.1"
                     /translation="MIGKIDLHTHTYFSDGQHSPEELIIKAKEAGIDVISVTDHDTVD
                     GIAETIEAGKRYGVEVIPGLEISSDIRDREVHLLAYFFNPANKQLEEYLKFFRAERIK
                     RASVIIEKLRELGLVIELDDVMEIARNSAVGRPHIAKALVRKGLVSNYFEAFSKYIGN
                     GCPAYERKVHVSPRSAFKIISDAGGLSFIAHPGHLPDVIMVELIEAGLDGIEVVHPSH
                     LPHQVKHYRGIVNEYFLLESGGSDFHGGERNDYSNLGKYSVSYSKIEAMRKQILRNTA
                     "
     gene            complement(2480..3163)
                     /locus_tag="VF870_08365"
     CDS             complement(2480..3163)
                     /locus_tag="VF870_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9252242.1"
                     /translation="MLNNTEFLANVPIFSDLDADTLEKIAKSGVLQSYKKNSIILSEE
                     DTGSALFVIAEGKVKISRSSNDEKEVILALLNQSDFFGEMSLLDGMARSATVTAVEDA
                     KLFIIQRAEFLDLLTKFPDVSVALLTELTKRLRNATMKIKALSLKDAEGKVATVLLQL
                     ADDVGMIRQGVVEIDHLPFQQELANMAGTSRETISRTLHSFAKKGLVELDGSKLRITD
                     YEKFKEMFN"
     gene            3383..>4552
                     /locus_tag="VF870_08370"
     CDS             3383..>4552
                     /locus_tag="VF870_08370"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX9252243.1"
                     /translation="MAMMAKMRSLAPAFILTVGGLFVLFMVISDSNVMEVLGGGSRTN
                     NIGVVNGEKITYQEFQTALDQQLENQKRQTGKDPEEAQIEQIREQVWDAVVTQKIFSH
                     LIKKYGITVTDQEIKDVILGDNPPDFLKQNFIDSLGNFNKQLYEQAIFDPQNKQALIQ
                     AEELVRQSRLTQKLQSMILASVNVGEDEVRRRFIEQNTNIEADYVLVDINLIKDADIK
                     VTDEDIRAYYDKNINTYKIPPQRKLKYVLFKNAPSAEDSQMVYKNLMNVKNTLSSDTL
                     GFVQLVNIYSENPYSKDTLSVSTLTPEVVSALNKSTVGEVVGPFITPQGYTLFRYDGS
                     VTTPEVFARASHILINQYGSDENNLAEANKLYNRLIAGENFAALAKEYSKDPGSAV"
BASE COUNT         1495 a          812 c          742 g         1503 t
ORIGIN      
        1 cattatatgt ttcccattca gagccatcat atttgtaaat ccctacaccc gtaacatctt
       61 tgccgcttgc tgaccatagg ttaccttctt tatcgacagt catgtttgga aattgatttg
      121 cagcaggacc atttggataa acaaaattat tgttaacatt gattcccaat ttagtagcga
      181 ctattaatcc tgcattttcc tgatagctaa gtttgactgg ctgagaatca gtgttccata
      241 attcagataa atcagttcca tcatatgtaa aaacctgagt gggtgtaaga atatttaatg
      301 aattacttgt taccagtagg tctaatattt tatttcccgc taaacttaaa aatggctgcc
      361 aagtatttcc atcaaaagaa tataacccgg aatttgttcc ggcaattaat gtgctgttgt
      421 agaatgataa aacattagct ccaccagttg gaagtccgct tgattgatca taaacattcc
      481 acgactcagg agcagataga ttagtagcgc ctgctttttg tatcgcaaca ccggattcag
      541 ttgccgcata aattaaatca gaaaataaaa cgttatttac ttttatgtta gaagtaaaac
      601 ttccgaactt aaaataagta tcataaaata tataattttt tgaatcgatt aaggaaattc
      661 caaagtcagt tgcaatgtaa acggtatcac cagaaatagt aatatcattt attcttttat
      721 ttgttttatc tgaattggaa atatccagta ttgaccggaa cgaatttgat gcaggatcat
      781 aaacatctat aagaccgctt gcgcttccaa accatacttt gccgtaataa tcaatgccga
      841 tagcagtaag ttcagttcca tttaatcccg aagctttact gtaagtttga aaactattat
      901 ccgatatgtt aaaaaaatat gcgccgcctt gcgaggcaac ccaaacacct gttgatgttg
      961 cagccagatc tttagctgat ttcatatcag cataattttg ccaattagat tgctgctgtg
     1021 caaacgaaga gattgtaaaa atacatgcaa ctatgattga tatgatattt ttcatttgaa
     1081 atcctaaata attaaacacg gtaaatgtta tatcgatatc ttctcaattg ttggaataaa
     1141 aatagaataa attattaata ttaaaagtag aaaaaatctg cctaaatgag tttaaataag
     1201 tctgccattt caagtgcagt taaagctgaa tcccagccct tgttgccggc tttagttccg
     1261 gctctctcaa ttgcctgctc aattgtatct gtggtcaaca caccgaaaat aaccggcaaa
     1321 gaatattttt gagaaacttg agaaatgccg gtgctggttg ttgaagccac atattcaaaa
     1381 tgcggggttg cacctctaat gattacacct aaacaaatta ttgaatcata ttttttctta
     1441 gatgctaatc tatcggcagt aacaggaatt tcaaatgctc ccggtacttt tattatgtca
     1501 atattatttt cagtaacacc atgtttaatt aaacaatcgt tggctgcttt aagtaatcca
     1561 tctcccacaa attcattaaa tctgctgaat attattgcaa actttttgcc gcttccgatt
     1621 agttttcctt caattgattt cattgttaag ctgtattcct taaaatttgt tttctcattg
     1681 cttctatttt agaatatgaa actgaatact ttcctaaatt agaataatca tttcgttcgc
     1741 cgccatgaaa atctgagccg ccgctttcaa gaagaaaata ttcattaaca attcctctgt
     1801 aatgttttac ctgatgcggt aggtgtgaag gatgcacaac ttcaatcccg tctaatccgg
     1861 cttcaattaa ttcaaccatt atcacatctg gtaaatgtcc cggatgtgca ataaaagata
     1921 atccacctgc atcgctgata attttgaaag cgcttcttgg tgaaacatgt acttttcttt
     1981 cataagcagg gcagccattc ccaatatact tgctgaaggc ttcaaagtaa tttgaaacta
     2041 atccttttct tacgagggct tttgcaatat gcggtctgcc aacggctgag tttcgtgcga
     2101 tttccattac atcgtctaat tcgataacta acccaagctc tcttaatttt tctattataa
     2161 cacttgcacg cttaattctt tctgcccgaa agaactttag atattcttcc aattgcttat
     2221 ttgcagggtt aaaaaaatat gcaagtaaat gaacttcccg gtccctgata tcagaactga
     2281 tctccaatcc gggtataact tcgacaccat atcttttgcc tgcttcgata gtttcagcaa
     2341 ttccatctac ggtatcgtga tcagtaacag aaataacatc aatccctgcc tctttagctt
     2401 ttataatcag ttcttcaggt gagtgctgtc catcagaaaa gtacgtgtgt gtatgtaaat
     2461 caatttttcc aatcataact caattgaaca tttctttaaa tttttcataa tcagtaattc
     2521 taagcttaga gccatctaat tcaactaaac ctttttttgc aaatgaatgg agagttcttg
     2581 aaattgtttc tcgggaagta ccagccatat tagccaactc ctgctgaaaa ggcaagtgat
     2641 caatttcaac aacaccttga cgaatcattc caacatcatc ggcaagttgt aatagtacag
     2701 tcgcaacttt accttcagca tctttaagtg aaagtgcttt aattttcatc gtggcatttc
     2761 tgagtctttt tgtcaactca gtaagtaatg caacagaaac atcggggaat tttgtaagca
     2821 aatctagaaa ttctgctctt tggataataa ataattttgc atcttcaacc gcagtaacag
     2881 ttgcagatct tgccataccg tcgagtaaag acatttcacc aaagaaatca gactgattta
     2941 atagtgccag tataacttct ttttcatcat tgctggatct ggaaattttt acttttcctt
     3001 ctgcaataac aaataaagcc gatcctgtat cctcttctga taaaattatg ctattctttt
     3061 tgtatgattg aagtacacct gatttagcaa ttttttcaag tgtatcagcg tccaaatcgg
     3121 aaaagattgg aacgtttgcg agaaattcag tattgtttaa caaaatatta cctctctttt
     3181 aaaataaaat catcaaaaaa tacccttgat gcatctcaaa gtcaacttta taaaatttat
     3241 aacttgcaat catactaatt aattgtccta aaggctttat ttaatgaata tttcaaagtt
     3301 ttttaattgt tttttagaag tgatgtctct attttaagcg gcttaaattt tcaaagttaa
     3361 ttaatacgga gatatagata ttatggctat gatggccaaa atgaggagtt tagctcctgc
     3421 ttttattcta acagttggcg gtctgttcgt actgtttatg gtaatttctg attcaaatgt
     3481 tatggaagta cttggcggcg gaagtagaac gaataacatt ggagtcgtta atggtgaaaa
     3541 aataacctac caggaatttc aaacagcatt agatcaacaa ttagaaaatc aaaaaagaca
     3601 aaccggtaaa gatcctgaag aggctcaaat tgaacagata agagagcaag tatgggatgc
     3661 tgttgtaact caaaaaatat tttctcattt aataaaaaaa tatgggataa cagttacaga
     3721 tcaggaaatt aaagacgtta ttttaggaga taatcccccg gatttcttga aacagaattt
     3781 tattgattca cttggtaact ttaataaaca gctttatgaa caggcaattt tcgatcccca
     3841 aaataaacaa gctttaatac aggcagaaga acttgtaaga caatcaagac ttactcaaaa
     3901 acttcagagt atgattttag cttctgttaa tgttggtgaa gatgaggtta gaagaagatt
     3961 tattgaacag aatacgaata ttgaggcaga ttatgtttta gtagatatta atcttataaa
     4021 agatgctgat attaaagtaa ccgatgaaga tataagagca tattatgata agaatatcaa
     4081 tacttataaa attcctcctc aaagaaaact aaaatatgtt ctctttaaaa acgctccatc
     4141 agcagaagat tctcagatgg tttataaaaa tctgatgaat gttaagaaca ctttatcaag
     4201 tgatactttg ggatttgttc agttggttaa tatttattcc gagaatcctt attctaaaga
     4261 taccttatca gtatctacat tgaccccgga agttgtttct gcattaaata aatccactgt
     4321 aggtgaggtt gttggacctt ttatcactcc acaaggttat acattattca ggtacgatgg
     4381 ttcagttacc actcccgaag tttttgctag agcatcacat atattgatca atcaatacgg
     4441 aagcgatgaa aacaatttag ctgaagcaaa taaattatat aacagattaa tcgcaggtga
     4501 aaattttgca gctctggcaa aagaatattc taaggatcca ggaagcgctg tt
//
LOCUS       DASWBY010000469         8150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_733208, whole genome shotgun sequence.
ACCESSION   DASWBY010000469 DASWBY010000000
VERSION     DASWBY010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733208"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VF870_08375"
     CDS             complement(<1..505)
                     /locus_tag="VF870_08375"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9252244.1"
                     /translation="MCLKKVFLSVVTLIFLLTPISYSQSITEKIDNLLKQYSEYRLFN
                     GSALVADNGEVIFKKGYGFANMEWNIPNSYDTKFRLGSVTKQFTSMLIMQLVEKNKIK
                     LEDKISDYLPYYRKDIGDKVTIEMLLTHTSGIPSYTSQPDFFDKTSKQYYAPDDFIKQ
                     YCSGDLEF"
     gene            complement(785..3961)
                     /locus_tag="VF870_08380"
     CDS             complement(785..3961)
                     /locus_tag="VF870_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252245.1"
                     /translation="MDKRLAVNALIAALIIFATCICTFPQSSTYKDRLGAVRDLVNSN
                     ELIMIWNQGETFGSQFCYQRIYDLNLTQPGGVDSTLIKKPLNIDSSIVGNRRLAVATG
                     NFLGGSFKHFVAAWEGPNNSITISIPEIQAGTLSWDNSNRLSVSGLASFGKRKIHVET
                     GNFLGNSQEEFLLAYEGMDTTVHLQLFSFNPGSLAPQERGSINDEKTLPSGSSLDNWD
                     IVAGDFDSDGYDDIALLFVKPMGGTNWSLFVKIYSINENGEFIPKSSKEVFQKPTYSV
                     LDINIDGAAGNFNADAGPEIAFGFSFFQGEEPGNDTYVYLIDVKNNLTSIIVDDTRRV
                     TRDVVGPNEMPQFNVGAGDLNGDYRDELILMSGSTFYVYTTDDQLLPQFRSQKSVLSV
                     GNNFNSDEFLAVEDMDLDRSSEIVVAKSFEDNEPGGLQHFELHVFSLDSTLINYTLKA
                     KRINEEPILSNNGSRNYAIALGDFDGDRIRLGDPVHYRKTGVMQPTVVLYTPPIHYDI
                     FDNTSYDLSGCYPDQNCGFFSTYIQSTTVDTTITTEINEDWGIDESLYIQLPMASLRT
                     KASYGDKFSRRESTSNSYTITTGRTAAGDDWIYANVFDIDFYEYPVYDSLNTTPIGHF
                     LVSIPGNRRPLWIEGKDDGVLGNLFRPNHEVGNILSYSTESAADTSRTIVNFNEQTIG
                     NTGTSHESLLLSTFQTNNIESSWNAGLEVGVTVGQIGSIGVGEFVSYSFPVGAEVGVT
                     GRYDYGEIYTQTVNVGHSLEVRSDFGHLQPQFGTSGTYYVTPYAYWTSFGALALDYKV
                     SPLPMGGNSFWQERYGGKPDLTFNLPWRYDTEKGFPMPGNDSTYRQRSRDIFLSKVEP
                     HGGDTVLIAARVRNYGLQSISTPFSVRFYTGDPNSGGAQIAETIVDSTIDARGSINVV
                     VPWTIPLSLPLANLRIYAVIDPDNSVTNEVHENNNIGWAPVIGYGSITNVESEIIIPD
                     KFVLHQAYPNPFNPTATISFELPTSENVSLKVFNTLGEEVETLVDEFQTAGKHNVKFN
                     GSRLASGVYFYRLQAGSFVQTQKMILLK"
     gene            complement(4230..4868)
                     /locus_tag="VF870_08385"
     CDS             complement(4230..4868)
                     /locus_tag="VF870_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252246.1"
                     /translation="MKIRNLHLLVLILFFTQIVLPQTTHDVSVGNFSFTPAQLTINVG
                     DIVRWTNTGGLHNVVADDNSFTSGAVSSSAWVYEHTFNSAGTNPYYCSLHGGPGGVGM
                     SGVVTVENATDVGDENISVLKFELDQNYPNPFNPSTNIQYAIASRQFVTLKVFDVLGN
                     EVATLVNEYKNAGSYDFEFKASVGSRQLANGVYLYRLQAGNFVQTRKMILLK"
     gene            complement(5263..6699)
                     /locus_tag="VF870_08390"
     CDS             complement(5263..6699)
                     /locus_tag="VF870_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9252247.1"
                     /translation="MNEEKELEITFNNQPPQRNSLWQELKDAIRGSEADYTEIKMGKA
                     IFLLAVPMILELIMESTFAVVDIFFVGKLGPSAVATVGLTETYLFLLYSIGMGLAMAV
                     TAIVARRIGEKEKEKAGISAVQSILLAILISLPFAAAGIFYSKELLAIMGGDEWVLTN
                     GYSYMQWMLGGNVVIMLLFIINAIFRGAGDAAIAMRVLWIANGINMVLDPVLIFGWGP
                     FPQLGIEGAAIATNIGRGVGVLTQLWFLFKGVKHIKVFRSQIHFHWRTIVQIFKTSLG
                     GVGQMIIAMTSWIFIMRILAEFGSEIIAGTTIAMRIMMFTMMPAWGMSNAVATLVGQN
                     LGAKKPDRAERSVWITGIWNMLFLVGVAVVYFTASESLVGIFTDDLKVISVGAMWLKI
                     VSYSYFVYGWWMVASQAFNGAGDTITPTKINFVFFWLIEIPLSYFMAKTLNMEYLGVF
                     WAIFIAETAVGLFTLWLFTRGKWKTVQV"
     gene            complement(6784..7134)
                     /locus_tag="VF870_08395"
     CDS             complement(6784..7134)
                     /locus_tag="VF870_08395"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9252248.1"
                     /translation="MSKNKNTVAAYMSAFEKGDRSKILSRLTDDVVWILPGVFHLKGK
                     KEFEGEIRNDAFQGDPEIKVIRMTEEDNIVVAEGFVRTQKKDGDFINLSFCDVFEMEN
                     EKIKKIISYLMETK"
     gene            complement(7153..7503)
                     /locus_tag="VF870_08400"
     CDS             complement(7153..7503)
                     /locus_tag="VF870_08400"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX9252249.1"
                     /translation="MNSAKNKFATIDEYSKTFPKYIQKILEQVRETIREAAFDAEETI
                     NYQIPTFKLNGNLVHFAAFKNQIGFYPISKTLEDFQKDLAKFKSSKGAVQFPINEPMP
                     LKLISKIVKYRVKK"
     gene            complement(7537..7986)
                     /locus_tag="VF870_08405"
     CDS             complement(7537..7986)
                     /locus_tag="VF870_08405"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9252250.1"
                     /translation="MQKLKFSIEINAPKEKVWDTMLDDKTYRQWTEPFSPGCYYEGDW
                     NKGSKILFLATDQEGKIGGMVSRIKDNIKHEYISIEHLGMVQDGVEDTTSDAVKPWAG
                     LLENYAFKSDGSRTELLVHINVNDDFKDMFDDMWPKALRKLKELAEK"
BASE COUNT         2533 a         1622 c         1281 g         2714 t
ORIGIN      
        1 caaattcaag gtcaccactg cagtattgct ttataaagtc atcaggtgca taatattgtt
       61 tacttgtttt atcaaaaaag tcaggttgac tagtgtaact tggaattccg gaggtgtgtg
      121 ttaatagcat ttcgattgta actttatctc caatatcttt cctgtagtat ggcaggtaat
      181 ctgatatttt atcttcaagc tttattttat ttttttcaac aagctgcata ataagcattg
      241 aagtaaattg tttggttaca gaacccaacc ggaattttgt atcgtaagag tttggaatat
      301 tccactccat atttgcgaat ccataacctt tcttaaatat cacttcccca ttatcagcta
      361 ctaaagcact gccgttaaaa agtcgatatt cggaatactg ttttagaagg ttatcaattt
      421 tttcagtgat actttgtgaa taagaaattg gggtgagtaa aaagatgagt gtgactacag
      481 atagaaatac tttttttaaa cacattttag tttttcctta agtcgtttat aaaaattaat
      541 atttcatatt attaactgat acaatattct aaatatttaa taacctattt gtcatataat
      601 gatagagggt gaaataaatt attgattggt taatttttgg attagacttt aaattcaaaa
      661 ttagaaaaaa taaaaaaccc tgaaaccagc ctgattaacc gcaagactaa aaagtttcag
      721 ggtcttaaac aaaatcgttt ttttttctct ttggattttc tttttacgtc ttatggctag
      781 cttctcactt caacagaatc atcttctgtg tctgcacaaa acttcctgcc tgcaatctat
      841 agaaatatac tccactcgcc agcctgcttc cattaaattt tacgttgtgt ttaccggcag
      901 tttgaaattc atcaaccagt gtttcgactt cttccccaag agtattaaac actttaagag
      961 atacattttc ggaagtagga agttcaaaac taattgtagc tgttggatta aatggattag
     1021 gatatgcctg atgcagaaca aatttatctg gtattataat ctcagattcg acattggtta
     1081 ttgaaccata accaattaca ggtgcccagc caatattatt attttcgtga acttcatttg
     1141 taaccgaatt atccggatca attacagcat atatccttaa attggccaat ggtagtgaaa
     1201 gcggaatggt ccaaggtaca acaacattta tagaaccgcg tgcatcgatt gttgaatcaa
     1261 ctattgtttc ggctatttga gcaccgcctg agtttggatc acctgtataa aaccttactg
     1321 aaaagggtgt ggaaatagac tgcaatccat aatttcttac cctcgctgca attagtactg
     1381 tatcacctcc atgcggttca acttttgaaa gaaaaatatc tcttgagcgc tgacgatatg
     1441 ttgaatcatt gccgggcatt ggaaatcctt tctcagtatc gtatctccat ggcaaattga
     1501 atgtaagatc aggtttaccg ccataccttt cctgccagaa tgaatttcca cccataggca
     1561 atggtgaaac tttataatct aatgcaagcg ccccaaaaga tgtccaatag gcataaggtg
     1621 ttacataata tgtaccagaa gtcccaaact gcggctgaag gtggccaaag tcactccgga
     1681 cttctaaaga atgcccaaca tttacagttt gtgtgtatat ctcaccatag tcgtacctgc
     1741 ctgtcactcc tacctcagct ccaacgggaa aagaatatga aacgaattcg ccaactccta
     1801 ttgatccgat ttgccccacc gttactccaa cctctaatcc tgcattccag ctcgattcta
     1861 tgttgtttgt ttgaaatgta ctaagcaaca gcgattcgtg actggtacca gtatttccaa
     1921 ttgtttgctc gttaaagttc acaattgttc ttgaagtgtc ggcagcactt tctgtactgt
     1981 aggaaaggat gttgccaacc tcatgatttg gccggaagag atttcccaaa accccatcat
     2041 ctttaccttc aatccataag ggtcgccgat tgcctggaat tgaaactaga aaatgaccaa
     2101 taggtgttgt attcaaactg tcatataccg ggtactcata aaagtctata tcaaatacat
     2161 tagcatatat ccaatcatca cccgccgctg ttctgccagt agttattgta taagagttcg
     2221 atgtggactc tcttctgctg aatttatcgc catacgacgc cttagtcctt aatgaagcca
     2281 tcggtagttg tatatataaa gactcatcaa taccccagtc ctcattaatt tctgtcgtaa
     2341 ttgttgtatc aacagttgtc gattggatat atgttgaaaa gaatccacag ttctgatcag
     2401 gataacaacc gcttaaatca taactcgtat tatcaaatat atcgtaatga ataggaggtg
     2461 tgtatagaac aactgtcggt tgcataactc cggtctttct ataatgcaca ggatcaccta
     2521 atcttattct gtccccatca aaatcaccta aagcaatagc ataatttctt gagccattat
     2581 tgctaagtat tggttcttca tttattcttt ttgcttttaa agtataattt ataagagtag
     2641 aatccaggct aaatacgtgc agttcaaaat gctgcagtcc ccctggctcg ttatcttcaa
     2701 aactttttgc cacaactatt tctgaactac gatcaagatc catatcttca acagcaagaa
     2761 actcatcgct attgaaatta tttcctacag ataaaacact tttttgtgat ctgaattgtg
     2821 gtaaaagttg atcatccgtt gtatagacat agaatgtact gcctgacatt agaataagtt
     2881 catctcgata atccccattt agatcacctg ctccaacatt gaactgaggc atttcatttg
     2941 gtccgactac atcccttgtc actcttcgag tatcatcgac aataattgaa gtaagattat
     3001 tttttacatc aataagatat acgtacgtat cgttacccgg ttcctcaccc tgaaaaaatg
     3061 aaaatccaaa cgctatctcc ggtccggcat cagcattaaa atttcccgca gcaccatcaa
     3121 tattaatatc tagcacagaa taagttggtt tttggaaaac ctcttttgaa gatttgggaa
     3181 tgaattcacc attttcatta atggaatata ttttaacaaa aagtgaccag tttgttccac
     3241 ccattggttt tacaaataaa agtgcaatat catcgtatcc atcgctatca aaatctcctg
     3301 caacaatatc ccagttatca agtgagctcc cgcttggtaa agttttttca tcattgatac
     3361 tgcctcgctc ttgtggggct aaactgccgg gattgaatga gaataattgt aaatgtacag
     3421 ttgtgtccat tccttcgtat gcaagtaaaa attcttcctg actatttcca agaaaattac
     3481 ctgtctcgac gtggattttt ctttttccaa atgatgcaag accggaaact gaaagtctat
     3541 tactattatc ccagctaaga gtgcccgctt gaatttcagg gatcgaaatc gtaattgaat
     3601 tattgggacc ttcccacgca gcaacaaaat gcttaaaact tcctcctaaa aagttgccgg
     3661 ttgctactgc taatcttcta ttcccaacaa tactagaatc aatatttaaa ggttttttaa
     3721 ttaatgtaga gtcaactcct cccggttggg tcaagttgag atcataaatt ctctgataac
     3781 aaaattgtga tccaaaagtc tctccctggt tccagatcat aataagttca ttagaattta
     3841 caagatccct tacagcacca agtcgatctt tataagttga agattgtggg aaagtgcaga
     3901 tacaagttgc gaagattata agtgctgcga ttaaagcatt aactgcaagt cttttatcca
     3961 taagttgcct ccttatagta agagattgat aatgattaat tttagtaatc gaattatttt
     4021 ttatattcag aaaactatgt aattactaac aattgtaatt atcattaatt taatcattat
     4081 tatataagaa ataattaaac ccactaattg aaatcaaata aaagaactga actgattagc
     4141 cgcaaggcta aataatttca aagtcctaaa caaaatcatc tttctttttt cgtcttttgt
     4201 ttctcgttat tcgtcttact actagcttat cacttcaaca aaatcatctt ccttgtctgc
     4261 acaaaatttc cagcttgcaa tctgtataaa taaactccgt ttgctaactg tctactgccg
     4321 actgaggcct tgaattcaaa atcataactt cccgcatttt tatattcatt aacaagtgtg
     4381 gcaacttcat ttccaaggac atcaaatact tttaatgtta caaattgtct gcttgctatt
     4441 gcatactgaa tattggtgct tggattgaat ggattaggat agttctgatc taattcaaat
     4501 ttcaatacgg aaatgttttc atctccaaca tcggtagcat tttcaactgt aactacacca
     4561 gacattccaa ctccaccggg tccaccgtgc aatgaacaat aataaggatt tgttcctgct
     4621 gaattaaatg tatgttcata aacccaggca ctcgaagata ctgcaccgct ggtaaaactg
     4681 ttatcatcgg caactacatt atgcaggccg cctgtatttg tccatcggac tatatctcca
     4741 acatttatgg ttaattgagc cggtgtgaaa gaaaaattac caacactaac atcgtgcgta
     4801 gtctggggca atactatttg agtaaaaaat agtattaaaa ccaatagatg tagatttctt
     4861 attttcatat agcctccaaa ggtcatatta tttaattgat taaatagggt catagttcat
     4921 atttcgttgc ataaataatc taaaataaaa ttttaaaaaa accatcctac tgttcttaaa
     4981 ataataattt taataaagat gtgttaggat taatattgca atctctagga tttttattgc
     5041 aaaattggtt ctgctgtatt ttaaaactgt ttcccgcctc aatagtgtat caaaatggca
     5101 ttaattcaga tggtattatt atccttttgg agtaaacata aataaattgg ctgtgattaa
     5161 aattggaacc tgcaggcgga aatagattcc ttaaaaggat tggtttatcc tgatcaggaa
     5221 aaagttagcc gtatttctgt tagaaattga taaagttttg agttaaacct gtactgtttt
     5281 ccatttacct cttgtaaaca accagagagt gaataatcca acagcagttt ctgcaataaa
     5341 gattgcccag aaaacaccta agtattccat attcaatgtc ttggccataa agtaagaaag
     5401 aggtatctca atcagccaga agaacacaaa atttatttta gttggtgtga tagtatcccc
     5461 ggcaccatta aatgcctgtg aggcaaccat ccaccatcca tatacaaaat aagaatacga
     5521 aacaatttta agccacattg ctccaactga aattactttc aggtcatccg taaatattcc
     5581 aactaaagat tcactcgctg taaaatatac tacagcaacg ccaaccagga ataacatatt
     5641 ccatatccca gtaatccaca ctgatctttc agcacggtca ggttttttgg ctccgagatt
     5701 ttgtcccaca agtgtagcaa ctgcattcga cattccccac gcaggcatca tcgtgaacat
     5761 cattattctc atcgcaattg tagttccagc gattatttcg cttccaaact cagcgaggat
     5821 tctcataata aatatccacg aagtcattgc aatgatcatt tgtccaacgc cgcctagaga
     5881 agttttaaat atttgaacaa ttgttctcca gtgaaaatgt atttgagatc gaaaaacttt
     5941 aatgtgttta actcctttaa aaagaaacca gagctgagta agaacaccta cgcctctacc
     6001 aatgtttgtt gcaatcgcag ccccttcaat tccaagctga gggaatggtc cccacccgaa
     6061 aatcaaaaca gggtctaaaa ccatattaat cccattcgca atccagagaa cacgcatggc
     6121 aatagcagca tcaccggcgc ctctgaatat tgcattgata ataaacagaa gcataataac
     6181 tacatttcct ccaagcatcc attgcatata actgtagcca tttgtaagca cccactcatc
     6241 gccacccatt atagcaagca gttcttttga atagaatatc ccagcagcgg caaaaggaag
     6301 tgaaattaat atcgcaagta aaatcgattg cacagcactg ataccggctt tctctttttc
     6361 cttctcacca attcttcttg caacaatagc agtgacggcc atcgccaatc ccattccaat
     6421 agaataaagc aggaacaagt aggtctctgt taatccaact gttgcaactg cagaaggacc
     6481 gagtttacca acaaaaaaaa tatccaccac agcaaaagtt gattccatta ttaattccag
     6541 aatcatcgga actgcgagaa gaaaaattgc tttgcccatt tttatttcag tataatcggc
     6601 ctcgcttccg cggattgcat cttttaattc ctgccaaaga gaatttcttt gtggaggttg
     6661 attattaaaa gttatttcta attctttttc ttcgttcata tccaaattaa ttggttgaat
     6721 tgtttcattg ctaaaagtgg aattatacaa aatgatattt aatggttaat tcctctcatt
     6781 atttcatttt gtttccatca agtaagaaat tattttttta attttttcat tttccatttc
     6841 aaagacatca cagaaagata gattgataaa atcaccatct ttcttttgtg tccggacaaa
     6901 accttctgca acaacaatat tatcttcctc tgtcattctt ataactttta tttccggatc
     6961 tccctgaaat gcatcatttc ggatttcacc ttcaaattct tttttacctt taaggtgaaa
     7021 aacaccgggt aaaatccaca cgacatcatc tgtcagacga gaaaggattt ttgagcgatc
     7081 acctttttca aatgcactca tataagctgc gactgtattt ttatttttag acatttctat
     7141 tttttattcc tttcattttt taactctata cttcacaatt ttcgaaatca gttttaatgg
     7201 cattggttcg ttaattggga attgaactgc acctttagat gatttgaatt tagctaaatc
     7261 tttctgaaaa tcttctaggg tttttgagat cggataaaac ccaatttgat ttttaaatgc
     7321 agcaaagtgg acaagattac cattcagctt aaatgtcggt atctgatagt tgatagtttc
     7381 ttctgcatca aaagcagctt ctctaatcgt ctctcttacc tgttccagaa tcttttgaat
     7441 atattttgga aaagttttgc tgtattcatc aattgttgca aatttatttt ttgctgaatt
     7501 cataattgat tattcctgat ctaaataaag ttaaaattat ttttcagcaa gctctttcag
     7561 tttccgaagt gctttaggcc acatatcatc gaacatatct ttaaaatcat cattgacatt
     7621 tatatgaaca agcagttctg ttctgcttcc gtcactttta aatgcataat tttcaagtaa
     7681 accggcccat ggttttaccg catcacttgt tgtgtcttct accccatcct gaaccatacc
     7741 aagatgttcg attgaaatat attcgtgctt gatgttatct tttatccggc tgaccattcc
     7801 tccaatttta ccctcttggt ccgttgcaag aaaaagtatt ttgctgcctt tgttccagtc
     7861 accttcataa taacaaccgg gtgaaaatgg ttcagtccat tgacggtaag ttttatcatc
     7921 aagcattgtg tcccagactt tttccttggg tgcattgatc tcaattgaaa attttagttt
     7981 ttgcatgatc agtctcctta tcgcttcttt atagttttta aagaattttc tttcactctt
     8041 tgttttacaa ttctttgaat taatattaat ggcatcggct tatcaattgg aaattgaata
     8101 gcgcctttcg atgatttgaa tttaactaaa tctttctgaa aatcttctat
//
LOCUS       DASWBY010000470         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_21688, whole genome shotgun sequence.
ACCESSION   DASWBY010000470 DASWBY010000000
VERSION     DASWBY010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21688"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VF870_08410"
     CDS             <1..597
                     /locus_tag="VF870_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX9252251.1"
                     /translation="DSVSLPVTVKTRIGWDNNSIQIVEVAKRLEDVGIKALTVHCRTR
                     VMGHKGDADWSWIPKVKEAVSIPIVLNGNVLDATDVKQAFNETNADGVMIARGAIGNP
                     WIFLEAKEILEKGYISTKIDDDMKIKTCLRHLDLAIGVKGERRAVIEHRKFYSGYLKG
                     MHHASKIRNELMQFIDYNPVKETLLRYLDELKKTELSY"
     gene            758..1357
                     /locus_tag="VF870_08415"
     CDS             758..1357
                     /locus_tag="VF870_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9252252.1"
                     /translation="MKLFTSHKLARFISTLFVPPSFTIIVYAIFTFTLENDSANRLLT
                     FIIPLVFGFIAPIVLFIILRKKGKLADQDASIKEERTFPFLVAIGFYLFGLLLMIKFD
                     LNIISIAFWFCYISNTLITIIINKYWKISAHAMGAAGPFAAITFTLGWLGLLMLPVVI
                     LVGWSRIELKCHTFAQVVAGIILAFSSVYLQMFLITKYY"
     gene            1490..>2161
                     /locus_tag="VF870_08420"
     CDS             1490..>2161
                     /locus_tag="VF870_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9252253.1"
                     /translation="MIKSEIKNNTATITLNRPEKRNALHPDLVLQLKAKLIEFEKDEN
                     IKSLIITGEGNAFCAGADLEYLQQLSTNSTIENYEDSKSLAELFLMIYEFHKPTIAAV
                     NGAAIAGGCGLASVCDFIIAHPEKSKFGYSEVKIGFIPAIVSIFLIKKIGEGLANQLL
                     LEGEIISGKRAYEIGFANYLAEDVLSESLKLAERLNKNSSESIRLTKQMIHNLSNLAV
                     REAVNY"
BASE COUNT          746 a          315 c          396 g          704 t
ORIGIN      
        1 gattcagttt ctctgcctgt tacagttaaa acaagaatcg gctgggataa taattcaatt
       61 caaattgttg aagttgcaaa gcggcttgag gatgttggaa taaaagcctt aacagttcat
      121 tgtagaaccc gcgttatggg gcacaaaggt gatgctgact ggagctggat cccaaaagtt
      181 aaagaagctg tttcaattcc aatagtttta aacggaaatg ttcttgatgc tacagatgtt
      241 aaacaagctt ttaatgaaac caatgctgat ggtgttatga ttgcccgcgg tgctatcgga
      301 aatccctgga tttttttaga agcaaaagag attcttgaaa agggttacat ctcaactaag
      361 attgatgatg acatgaaaat taaaacatgt ttacgccatt tagatttagc aattggagtc
      421 aaaggtgagc ggcgcgcagt tattgagcat agaaaatttt atagtggata tttaaaagga
      481 atgcatcacg catccaaaat aagaaatgaa ttaatgcagt ttatagatta taatccggtt
      541 aaagaaactc tgttaagata tttggatgaa ctaaaaaaga cagagttatc ttattaatta
      601 ttccaccttt cgttgtcctg agagaagtcg aaggatgacc aataccgatt ctttgataaa
      661 tgatcactct aggtcctggc tgccagtagg caagtttcga tcaatgtaac attacagaat
      721 atttaatttt attaaagtgt aattagatac catattattg aaattattca catcacataa
      781 actcgcacga tttatttcaa ctctatttgt tcctccatca tttacaatta ttgtttatgc
      841 aatctttaca tttacacttg aaaatgattc agctaacaga ttattaactt tcatcatccc
      901 attagtattt ggattcattg ccccaattgt cctttttata attttaagaa aaaaaggaaa
      961 acttgctgac caggatgcat caataaagga ggagagaacc tttccatttt tggttgcaat
     1021 tggattttat ctatttggat tattgttgat gataaaattt gatctgaata ttatttcaat
     1081 cgcattttgg ttttgttata tttctaacac cttaattaca attattatta ataaatattg
     1141 gaaaatcagt gcgcacgcca tgggtgcagc cggaccattt gcagcaatta cttttacttt
     1201 gggttggtta ggattactga tgctgccagt tgtaatactt gttggatggt caagaattga
     1261 attgaagtgt catacctttg ctcaagttgt tgcgggaatt atattggcat tctcttcagt
     1321 atatttgcag atgtttttga taacgaaata ttattaaaaa gttatttcga aggcgtcttc
     1381 gactgagaaa tctaaaaatg aatgctttag tatttcttcc tttggtcgaa atgacatttt
     1441 ctagaataga aataattata agtggaacga ataaaattat aaatcaaaga tgattaaatc
     1501 agaaataaaa aataacactg ccaccattac cctaaatcgg cccgaaaaga gaaatgctct
     1561 tcatccggat ttagttttgc aattgaaagc caaactaatt gaatttgaaa aagatgaaaa
     1621 tattaaatca ttgattataa caggggaggg aaatgctttt tgtgccggag cagatttaga
     1681 atacctccaa cagctatcta ctaactcaac aatagaaaat tatgaggatt ctaaatcttt
     1741 agcagaattg tttttaatga tttatgaatt tcataaacca actattgcag ctgtaaatgg
     1801 agctgcaatt gcaggcggtt gtggactagc atcagtttgt gatttcataa tcgcacatcc
     1861 tgagaaaagt aaatttggat acagcgaagt aaaaattgga ttcattccgg caatagtttc
     1921 aatttttcta attaaaaaaa tcggcgaagg attggcaaat caattattat tagaaggtga
     1981 aatcatctca ggaaaaagag cttatgaaat tggctttgca aattatcttg cagaggatgt
     2041 attgagcgaa tcattaaaac ttgccgagag attaaataaa aattcaagtg aaagcataag
     2101 attaacaaaa caaatgattc ataatctttc gaatttagca gtaagagaag ctgttaatta
     2161 t
//
LOCUS       DASWBY010000471         5288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_55031, whole genome shotgun sequence.
ACCESSION   DASWBY010000471 DASWBY010000000
VERSION     DASWBY010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55031"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VF870_08425"
     CDS             <1..762
                     /locus_tag="VF870_08425"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9252254.1"
                     /translation="YYIIPIRKDVVLDNLKHAFPDLSPKEIKDIAYGSYRSFAITLIE
                     ILYLPRINKDPIKNLVKINNLELVEKKEKEKNGLLLLTAHFGNWEYFAISTGLKLNKN
                     LSVIVKSQRNPYVNNWMNKARTRLVNEVVPLGISIRNVYAALLRKGIIGLVADQRGPE
                     ESIKLNFFGRKTSVYTGPAILSLKMNSPIIYGVAIRQPDYSYKVDFVEVSKEGLPDDY
                     DGKVKVLSERLLKLLEESIREHPEQWLWMHKRWKH"
     gene            764..1789
                     /locus_tag="VF870_08430"
     CDS             764..1789
                     /locus_tag="VF870_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9252255.1"
                     /translation="MQNERILYIQTAFPGDAILILPSLKKLKDFFPESNIDVLCIPAT
                     KEIFSASPYVDNAIVIDKKGKEKTLLSTYKFIKQLKQNNYSRIYSSHRSLRTSLIVLL
                     LEVKETFGFDNSALIHVYKNLIHYQSEKHEVQRNFDLIGFEYDDQSWRVTPEIISGEQ
                     SAQKVKLFIKENNLTNGFIAIAPGSVWNTKKYPSDYYEIIIKYFVDRKNKLVLIGGEN
                     DKSITEDIASKFSENVVNSTGSFSIVESIELLKGAKLLISNDSAPTHMGMCADIKVLT
                     IYCSTVPEFGFYPYNNKSSFISFDDLNCKPCGIHGFDKCPIKTFDCAKKLLPDQIISK
                     VEEMLND"
     gene            1782..2417
                     /locus_tag="VF870_08435"
     CDS             1782..2417
                     /locus_tag="VF870_08435"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX9252256.1"
                     /translation="MTNKKKAKLINIDENFEESINLASELFMKGSIFIYPTDTIYGFG
                     GNPFNENAIKKISDVKGKENWRRYIFLISEIEMLKSYADINSEKYLDFLLSIWPNPVS
                     VILKLNKKYQQILKSETGAFRIPNHRFCVKLISEINMPLVSTSVNRTGKEPLNDPSLI
                     IQEFGNEVDAIFYSDKKSFFEASTLIDLVKDEPLLIREGRIKFVELMKKLN"
     gene            2442..3260
                     /locus_tag="VF870_08440"
     CDS             2442..3260
                     /locus_tag="VF870_08440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9252257.1"
                     /translation="MLKLFKIKKFSIIVLPEATSSEPRSIKISFAKLLTYIGVYSVII
                     FLLGFYIISFTPLSELLFPYSLRMTESDKKKVEILNNKVVFLAKEIESLKSVNERLKF
                     AIMLGDSTLLNEPLPKRDSANTKIPGEGNILSVFIKFYDKMFSTQNEDIFFIKPAEGF
                     LSRSFDPERGHFGNDYSMKENNPIFASAGGYVVFADYTTAYGYSIIINHQENYVTKYL
                     HCSSLLKKAGDFVRQGEMIALSGNSGTESSGPHLHFEIWKDGKPIDPSKILINF"
     gene            3271..3648
                     /locus_tag="VF870_08445"
     CDS             3271..3648
                     /locus_tag="VF870_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9252258.1"
                     /translation="MKTKSNGSAMEEVTIISSGVVIEGKVTSNGNVRVDGTVKGDITA
                     QGNLTVGEHGSIQGQLTGETVSIGGKVEGTVNAKEKLVLESKAVLKGDLLTKILVVEA
                     GAMFEGKSSMSSQSSFKPVSKGE"
     gene            3650..3910
                     /locus_tag="VF870_08450"
     CDS             3650..3910
                     /locus_tag="VF870_08450"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX9252259.1"
                     /translation="MALKPEDDSGVAKSLRDVGPYLGLGLQLAVTIVASVLIGSWLDK
                     KFSQNYVFTLIFGVFGIGIGLYNLIRTVTYLEKRNKVKNEKK"
     gene            4289..5131
                     /gene="atpB"
                     /locus_tag="VF870_08455"
     CDS             4289..5131
                     /gene="atpB"
                     /locus_tag="VF870_08455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9252260.1"
                     /translation="MIDSTSTQIIVDSLKSAKTSSSSDWILHHVMDGNYLDFSPFFKL
                     YLPHFELFGLDLSITRHVLFMWLGSILLFVVMTRVAKAYKSSMVPKGFTNFWELFIIF
                     VRDEIAMPTIGKGFEKFLPYLLTAFFFILFGNFLGLIPFSATFTSNIAVTATLAIFSF
                     IVIQLGGMRNNGAFGYFKGLIPHGVPVFLLPIMIVVEFLGLLAKPFALAIRLFANMTA
                     GHIVIYALISLIFVMQSVGWAGLAIPMALFIYALEILVALLQAYIFTMLSSLFIGMAV
                     HQDH"
     gene            5172..>5288
                     /locus_tag="VF870_08460"
     CDS             5172..>5288
                     /locus_tag="VF870_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX9252261.1"
                     /translation="MDFAYLAAGLGAGLTIFGGALGIGKLAAAAMDASGRQPE"
BASE COUNT         1743 a          750 c          987 g         1808 t
ORIGIN      
        1 tattatatca ttcctatccg taaggatgtt gtattagata acctaaagca tgcgtttcct
       61 gatttatcgc cgaaagaaat taaagatatt gcttatggca gttacaggag ttttgcaatt
      121 actcttattg aaattttata tctgccaagg ataaacaaag atccgatcaa aaatttagtt
      181 aagattaata atctggaatt agttgaaaaa aaggaaaaag aaaaaaatgg gctcttacta
      241 ttaacagcac attttggtaa ttgggagtat ttcgcaattt caacaggatt aaaactgaac
      301 aaaaatttat cagtaattgt taagtcacaa cgcaatccct atgttaataa ttggatgaat
      361 aaagcccgta caagattagt taatgaagta gtgcctttgg gcatttcaat tagaaatgta
      421 tatgcagcac ttttaagaaa aggcataatt ggattggttg ctgatcaaag agggcccgaa
      481 gagagtatta aattaaattt ttttggacgt aaaacatcgg tttatacagg acctgcaatt
      541 ttatcactaa aaatgaattc gccaataatt tatggggtag ccatcaggca gcctgattat
      601 tcatacaaag ttgactttgt tgaagtaagt aaagaaggtt tgccggatga ttatgatgga
      661 aaggttaaag tactttctga aagattatta aaacttcttg aagaaagcat tcgtgagcat
      721 cctgaacaat ggttatggat gcataaaagg tggaagcatt aatatgcaaa acgaaaggat
      781 tctctatata caaacagcgt ttccaggtga tgcaatcctt atactgccgt cacttaagaa
      841 actcaaagat ttttttccgg aaagtaatat tgatgtgctg tgtattcccg ctaccaaaga
      901 aattttttct gcttctcctt atgttgataa tgcaattgta atagataaaa aaggcaaaga
      961 aaaaacttta ttaagcactt ataaatttat taagcagtta aagcaaaaca attattcaag
     1021 aatttattct tcgcaccgat cacttcgaac atcattaatt gttctattgc ttgaagtaaa
     1081 ggaaactttt ggttttgaca attcagctct tatacacgta tataagaact taattcatta
     1141 tcaatctgaa aagcatgaag tgcaaagaaa ttttgatctg attggatttg aatacgatga
     1201 tcaaagttgg agagtaaccc ctgaaattat ttctggtgaa caatcagctc aaaaagttaa
     1261 attgtttatt aaagaaaata atttaactaa tggattcatt gcaattgccc cgggcagtgt
     1321 ctggaataca aaaaaatatc cgtcagatta ttatgagatt atcataaaat attttgtaga
     1381 tcgaaaaaat aaacttgtat taatcggcgg cgaaaatgat aaatcaatta cggaagacat
     1441 tgcttcaaaa ttttctgaaa atgttgtcaa ttcgaccgga agtttttcaa ttgtggaaag
     1501 catcgaactc ttaaaaggtg caaagttatt gatttcaaat gatagcgcac caacgcatat
     1561 gggtatgtgt gcagatataa aagttttaac aatttattgt tcaactgtgc cggaatttgg
     1621 attttatcca tataacaata aaagttcatt cataagtttt gatgatttga attgcaagcc
     1681 gtgcggaatc cacggttttg ataagtgtcc aattaaaact tttgattgcg caaaaaagct
     1741 cttacctgat caaattattt ctaaagtgga ggaaatgtta aatgactaat aagaaaaaag
     1801 ccaaactaat taatattgat gaaaactttg aagaaagtat taatcttgcg agtgaacttt
     1861 ttatgaaagg ttcaattttt atttatccta ctgatacaat ctatggattt ggtggaaatc
     1921 cttttaatga aaacgctata aaaaaaatta gtgatgtaaa gggaaaagag aattggagac
     1981 gatatatatt tttaatttct gaaattgaaa tgttaaaaag ttatgctgat ataaattctg
     2041 agaaatattt agactttctt ttatctatat ggccaaatcc cgtttctgtg attcttaaac
     2101 taaataaaaa atatcagcag atactcaaat ctgaaacagg cgcatttaga attcctaacc
     2161 acagattttg tgttaaatta attagtgaaa taaacatgcc attggtatca accagtgtaa
     2221 acagaacagg taaagagcca ttgaatgatc cttcattaat tatacaggaa tttggaaatg
     2281 aagttgatgc aattttttat tctgataaaa aatcattttt tgaagcctca acgcttatcg
     2341 atttagttaa agatgagcct cttcttatta gggagggcag aattaaattt gtggaattaa
     2401 tgaaaaagct gaattagatt aaaaagtaaa taaagaatca gatgttaaaa ctatttaaaa
     2461 ttaaaaaatt ttcgataatc gttcttcctg aagcaacatc aagtgaacca cgatcgataa
     2521 aaatctcatt tgcaaaattg ttgacgtaca ttggggttta cagcgtgata atttttcttc
     2581 ttggttttta tataatcagt tttactcctc tcagtgagct tctgtttcca tattcattaa
     2641 gaatgacgga atccgataaa aagaaagtcg agattttaaa caacaaagtt gtttttcttg
     2701 ctaaagaaat tgagtcttta aaatctgtta atgagcgatt gaaatttgct ataatgttag
     2761 gtgattcaac tctattaaat gagcctctgc caaaaagaga ttctgcaaat actaagatac
     2821 ctggtgaagg aaatattcta agtgtattta ttaaatttta tgataaaatg ttttctaccc
     2881 aaaatgagga catctttttt attaaacctg ctgaaggctt tttaagcaga tcttttgatc
     2941 ccgaacgcgg gcacttcgga aatgattatt ctatgaaaga aaataatcct atctttgcat
     3001 cagcgggtgg ttatgtggta tttgccgatt atacaacagc ttatggttac tctatcataa
     3061 ttaaccatca ggaaaattat gtaactaaat atcttcattg ttcatcactg cttaaaaaag
     3121 caggtgactt tgtgagacaa ggcgagatga ttgctttaag cggaaattca ggtacagaat
     3181 catctggtcc gcatctgcat tttgaaatat ggaaagatgg aaaaccaata gatccatcaa
     3241 aaattttaat taatttttag gagttttatt ttgaaaacta aatcaaacgg gagtgctatg
     3301 gaagaagtaa caattataag cagcggtgtt gttattgagg gtaaagttac tagtaatggt
     3361 aatgtgcgtg ttgatggaac tgttaaagga gacatcacag cccagggtaa tcttactgtt
     3421 ggagagcacg gcagcataca gggtcaacta acaggtgaaa ctgtttcaat cggtggtaag
     3481 gttgaaggaa ctgttaatgc aaaagaaaaa cttgttctgg agtctaaagc agttttgaaa
     3541 ggtgatctgc ttacaaaaat acttgtcgta gaagctggag ctatgtttga aggaaaaagc
     3601 tctatgagca gccagtcatc ttttaagcca gtcagcaaag gagagtaaag tggctttaaa
     3661 gccggaagat gattccggtg tcgccaagtc gttgcgagat gttggtccct acctgggatt
     3721 aggtcttcaa cttgctgtaa caattgttgc ctctgtgttg ataggaagct ggctcgataa
     3781 gaagttctct cagaattatg tatttacgct aatttttggt gtatttggga ttggaattgg
     3841 actttataat ctgattagga cagtgacata tctggagaag cggaataaag taaagaatga
     3901 aaaaaaataa aagtgtttac tttttcggca ttataccatt tctatttttt attggattat
     3961 ctgcttgttt tttaagtaaa tgtattaatc ctgatctgtt ttttagtctt ttttgcggct
     4021 ttattttctc aacattcaat ttcttcctag gtgttctatc aattcatttt ggatttgaaa
     4081 aaagtgacaa aatctttcta attattgtgt tcgggggatt ggttgtacgc ctttttttga
     4141 tgctcatcct gattgtaata gccttaaaat tcttgtttgt taggctcaat tcttttatct
     4201 ttacaacctt tattttctat ttctactatc tcatagtgga gatttttata ctaaatcaga
     4261 aaaagaattt taatatcaag actaagcaat gattgattct acatcaacac aaataattgt
     4321 tgatagcctt aaaagtgcca aaacatcttc cagcagtgat tggatacttc atcacgtgat
     4381 ggatggtaat tatcttgatt tttcaccgtt ttttaagtta tatcttccac attttgagtt
     4441 atttggtctt gatctatcaa ttaccaggca cgtacttttt atgtggcttg gctccatttt
     4501 actttttgtt gtgatgacga gggttgctaa agcctacaaa tcatcgatgg tgcctaaagg
     4561 ttttacgaat ttctgggaat tgtttataat atttgttcgt gatgaaattg caatgccaac
     4621 tatcggaaaa gggttcgaaa agtttttgcc ttatctctta acggcttttt tctttattct
     4681 ttttggaaat tttttaggat taattccttt ttcagcaacg tttacaagta atatagctgt
     4741 tacagccact ttggctatct tttcttttat tgtaatacag cttggcggta tgagaaacaa
     4801 tggagcattt ggttacttta aaggattaat tcctcatggt gttccggttt tcttgttgcc
     4861 aattatgatt gtggttgaat ttttaggatt acttgcaaag ccatttgcat tggctatacg
     4921 tctttttgca aatatgactg caggccatat tgttatttat gcattgataa gtttgatatt
     4981 cgttatgcag agtgttggtt gggcaggtct ggcaattcct atggctttat ttatatacgc
     5041 acttgaaatt ttagtcgcct tattacaggc atatattttt acaatgctgt cctccttgtt
     5101 tataggaatg gcagttcatc aggatcatta agataattta taacattaaa tataattaaa
     5161 ggagaacgtt aatggacttt gcatatttgg ctgccggttt aggtgctgga ttaacaatct
     5221 ttggtggtgc tttaggcatt ggcaaattag ctgctgctgc aatggatgca agcggaagac
     5281 agccagaa
//
LOCUS       DASWBY010000472         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_132521, whole genome shotgun sequence.
ACCESSION   DASWBY010000472 DASWBY010000000
VERSION     DASWBY010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132521"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VF870_08465"
     CDS             <1..1320
                     /locus_tag="VF870_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252262.1"
                     /translation="LRYSYVDTDVQNGQTYYYACISYDQGDPNRGTKGLQPTECTKII
                     QQDYAGNVQFVDYNCAVVIPNAPVAGYVPPQIVGDTKEVNDGVGTGSMSVNILNPNAI
                     LDDARYDIVFKSTDDYPLYKTASYNVVRTFEGTTDTIIVNEDTTTIGEGRVSQPFDGM
                     AVSIINDTTVSVVDSLTTWLVGESNLTMRAYENKSSRGVPWPADYEINFSDVPLDTCY
                     LSSPPFYRKFPVNLKIFNRTEGKYSKVAVFDADASQSLTVGDTIQVLEFKDGVISTAN
                     VRFAWNLTYGFPVNPNETPIEPVNGDKFQIMTRKAFKTGDYFTYSTVPVLVDKSAAQN
                     SLSKIDVVPNPYLGAATWEKRNLNSSGRGERKIDFINLPAQCTVRIYTIAGALIKTLT
                     KDSAFSDGSLSWNLVTEDGMDAAYGVYLYHVDAPGVGEHIGKFALIK"
     gene            1334..>1889
                     /locus_tag="VF870_08470"
     CDS             1334..>1889
                     /locus_tag="VF870_08470"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9252263.1"
                     /translation="MKNKILIIFMLITLILPQSFAQSGFVSDVSKKGTTAAPFLSIGQ
                     SARAIGMGSAFVGVANDVSSIYWNPAGLTKTQGVNVAFDHTLWIADIKYNYLAASYSL
                     GGIGTIGFSYTGSDIGEMKVTTISNPEGTGETFSSTQAAFSLAYAVQLTEDFSIGFAH
                     KFIYESIWQMSANAFALDLGVQYVT"
BASE COUNT          584 a          324 c          391 g          590 t
ORIGIN      
        1 ttaagatatt catatgttga tactgatgta caaaacggac agacatacta ttatgcttgt
       61 atttcttatg atcagggtga cccgaatcga ggaactaaag gattgcagcc gactgaatgt
      121 acaaaaatta ttcagcagga ttatgcagga aatgttcagt ttgttgatta taactgtgcg
      181 gtggtaattc caaacgcacc ggttgctggt tatgtgccac cgcagattgt tggagataca
      241 aaagaagtta atgatggagt tggtaccgga tctatgagtg taaatatatt aaaccctaat
      301 gctatacttg atgatgcccg atacgatatt gtatttaaat cgactgatga ttatccgctc
      361 tataaaacag cttcgtacaa tgttgttaga acttttgaag gaactacaga tacaattatc
      421 gtaaatgaag atacaactac cataggagaa ggaagagtaa gtcagccatt tgatggtatg
      481 gctgtttcaa taattaacga tacaacagtt tctgttgttg attcattaac aacgtggctt
      541 gttggtgaaa gtaatttaac gatgagagcc tacgagaata agagctctcg aggtgttcca
      601 tggcctgcag attatgaaat taacttttct gatgtgcctt tggatacttg ttacttatct
      661 tctccgccat tttatagaaa atttccggta aatcttaaaa tttttaatcg aacagaagga
      721 aaatactcca aggtagcagt atttgatgca gatgcttctc agtctttaac tgttggtgat
      781 acaatccagg ttcttgaatt taaagatggt gttataagta ctgctaatgt gcgttttgca
      841 tggaacctta cttatggatt tcccgtaaat ccaaacgaga ctccaattga accggttaac
      901 ggagataaat ttcagataat gacacgtaaa gcatttaaga cgggagatta tttcacttat
      961 tctaccgttc cggttttggt ggataaatca gctgctcaaa acagtctttc aaagattgat
     1021 gttgttccaa atccttacct tggtgcagct acgtgggaaa aaagaaatct taactcatca
     1081 ggcagaggtg aaagaaaaat tgatttcata aatcttcctg cacaatgcac agtgcgtatt
     1141 tatacaattg cgggtgcatt aattaaaaca ctaacaaaag attccgcatt ttcggatggc
     1201 tcactttcct ggaatctggt tacagaagat ggtatggatg cagcatacgg agtatatctc
     1261 tatcacgttg atgctcctgg tgttggtgaa catatcggca aatttgcttt aattaaatag
     1321 cggagatatt gaaatgaaaa acaaaatatt aataattttt atgctgatca ctttgatact
     1381 tccgcagagt tttgctcaat caggctttgt ttcggatgta tctaaaaagg gaactacagc
     1441 cgcaccattc ctctcaatcg gacaaagtgc ccgtgcaatt ggaatgggca gtgcattcgt
     1501 tggcgttgcc aatgatgtta gttcaatata ctggaatcct gcaggcttaa ctaaaaccca
     1561 gggcgttaac gttgcatttg atcacacttt atggattgca gatataaaat acaactactt
     1621 agccgcaagc tatagcttag gtggcattgg tacaatcgga tttagttaca cgggttctga
     1681 tataggtgaa atgaaagtta caacaatttc aaatccggaa ggtaccggtg aaacattttc
     1741 atctacacaa gctgcattta gtttagcata tgctgttcag ctaaccgagg atttttcaat
     1801 cggttttgct cataaattca tctacgaaag tatctggcag atgagtgcta atgcatttgc
     1861 tttagatctt ggtgtacaat atgtcactc
//
LOCUS       DASWBY010000473         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_733532, whole genome shotgun sequence.
ACCESSION   DASWBY010000473 DASWBY010000000
VERSION     DASWBY010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733532"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VF870_08475"
     CDS             complement(<1..572)
                     /locus_tag="VF870_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252264.1"
                     /translation="MVIRIISILMILLCITGCSKKNETTTPDYIWDIDKNGIPEFVKT
                     NYIELARIYRISKYRSSVGHDYSDASEQCRSLKHYFEPKADVDWTTIKIYAPVKGTIT
                     RAEQEWAGIKLEIESEEFPAFRFSIFHINTSKQYNINDKVVEGELLGTHFGSQTYSDI
                     SVIVNDPAKLGRMVSFFDVMTDALFKEFSDR"
     gene            1336..>1713
                     /locus_tag="VF870_08480"
     CDS             1336..>1713
                     /locus_tag="VF870_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252265.1"
                     /translation="MNKLSGNYLKIIKAFHIGLIAITFGGIIAIEVILFLKLSDQHLT
                     FSNNLDYVIFKLNNSIIYYSFLAIVTTTIIYGLFTSWGFLRFYWIIIKWVLLFVFVII
                     FILFFSPSINALASLSDSGMHLGV"
BASE COUNT          533 a          293 c          288 g          599 t
ORIGIN      
        1 cgatctgaaa actctttgaa cagggcatca gtcatgacat caaaaaatga aaccattctc
       61 ccgagctttg ccggatcgtt gacaataaca gatatatcgg aataagtctg tgatccgaaa
      121 tgagttccaa gtaactcacc ttcaactact ttatcgttaa tattgtattg ttttgaagtg
      181 ttgatatgga aaattgaaaa tctgaaagcc gggaactcct ccgattcaat ttccagtttt
      241 attcctgccc attcctgttc tgccctggtg attgttcctt ttacgggcgc atagatcttt
      301 attgtcgtcc aatccacgtc agctttaggt tcgaagtagt gtttcagact tctgcactgc
      361 tcagaggcat cagaataatc gtgtcccaca gaagaacggt actttgatat tctgtagatc
      421 ctggccagct cgatgtaatt cgtcttgaca aattcaggaa tgccattctt atcaatatcc
      481 cagatatagt caggtgtggt tgtttcattc tttttagagc accctgttat acacagcaaa
      541 atcatcaaaa ttgaaataat cctgataacc attggcgtta atcttacctg attggctcaa
      601 ctaattatct ggcaaggtat gcgatttttt ttatttccac tatagtttac agccgttccc
      661 ggtaaagatg gcattataac tttgaattgt aaatccggca ggatctatgt gcgggatttt
      721 ataatttaat gggtgacgga atcggagctg gttttttcaa cgcacgcgtt gtccgtgcat
      781 aatataattt attttacatt gcagccgtca ccggatgaat ctacatttgt cgcgggtctc
      841 taaaatctgg caactttctt caatcccctt caactatttt caggtggttg gcctataaac
      901 aaaattaagt ggttccgaaa aattcgtgac aggaagtact ttgaaaatat atcctgaaag
      961 attaagcaat tgttcaagaa tgcctaatga aagagaaaga attaataatc cctgaaacct
     1021 gtttgctttg atatttgatc ttgacttgaa aataaagaaa aaagatagga taaatcccaa
     1081 acaaatcccc agaaagataa aaagttccag accatcgatg tgtaaggtaa tgttcataaa
     1141 tcaaaattaa tgaacaattt cagtttggct aagaattaaa atggaatatc aaatttatct
     1201 gttcaaatag ctgattaact tgctgatata tatcatcttc ttgtttttaa atcaaactta
     1261 tttgtaaatt aggcttgatt gtctttaaaa ttagtaagta ttaatctctc tttacaatcg
     1321 agataaaaac tattaatgaa taaattatca ggaaattact taaaaattat taaagcgttc
     1381 catattggac taattgcaat aacttttggt ggtataattg caatagaagt tattcttttt
     1441 ttgaaattat cagatcagca tctgactttt tcaaataacc ttgactatgt aatttttaaa
     1501 cttaataata gtataattta ttatagtttt ctcgccattg ttaccacaac gataatttat
     1561 gggctcttta caagctgggg atttcttaga ttctactgga taatcattaa atgggtatta
     1621 ctatttgtat ttgttataat atttatttta tttttttctc cttcaataaa tgcacttgca
     1681 tctctttctg atagtggcat gcatttaggt gtg
//
LOCUS       DASWBY010000474         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_733565, whole genome shotgun sequence.
ACCESSION   DASWBY010000474 DASWBY010000000
VERSION     DASWBY010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733565"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..745)
                     /locus_tag="VF870_08485"
     CDS             complement(17..745)
                     /locus_tag="VF870_08485"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX9252266.1"
                     /translation="MGFNYVRFTSPGSQKKFNEDAVETVEVDGGILAVLCDGVGGDNG
                     GDLAARIALKSALYFFSANENDDYLTKLKLAIEESNSFVLNHSSTSFPLKNMATTLEI
                     IFIKDNIVYWGHIGDSRIYHLKSRRLNQITKDHSLVQKLLDEGYITHKQAANHPQKNV
                     IIKALGDNELIEPDVSKIKLNETEENKFFLCSDGVSNLISNAEIETFLILDDLEDIKN
                     KLTKMIKLRGAPDDYSFIIIEITK"
     gene            991..1072
                     /locus_tag="VF870_08490"
     tRNA            991..1072
                     /locus_tag="VF870_08490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1025..1027,aa:Leu,seq:tag)
     gene            1116..>1508
                     /locus_tag="VF870_08495"
     CDS             1116..>1508
                     /locus_tag="VF870_08495"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HEX9252267.1"
                     /translation="MEYNVVELNSSEKEVEIKLQYDEIQKEIEEEVRKQSKKIQIPGF
                     RKGKVPASMLKKMYGDALEYEASEKIANSFFWKVAEENQLKPIGQPTMTDLKFEPEKE
                     LLFKVKYETIPVLNVKDYKAISFDVPELI"
BASE COUNT          515 a          250 c          241 g          502 t
ORIGIN      
        1 tgtattcttc tgccgatcat ttagttattt caattatgat aaatgaatag tcatccggag
       61 caccacgaag tttgatcatt tttgttagtt tatttttaat atcttcaaga tcatcaagga
      121 ttaaaaatgt ttcgatctcc gcgttgctta tcaaatttga tacaccatct gaacatagaa
      181 aaaatttatt ctcttcagtc tcatttaatt taattttgct aacatcaggc tcaattaatt
      241 cattatcccc aagggcttta ataattacat ttttttgtgg atgattagct gcctgtttat
      301 gcgtgatata tccttcatcc aaaagttttt gaaccagtga atgatcttta gtaatctgat
      361 tcagtcttct agattttaag tgataaattc ttgaatcacc aatatgtccc caataaacaa
      421 tattatcctt gataaaaata atttctaaag ttgtggccat attttttaaa ggaaaacttg
      481 tggaggaatg attcagtaca aaactatttg attcttcaat tgcaagcttg agcttggtta
      541 aataatcatc attctcatta gcagaaaaaa aatacagtgc tgattttagg gcaatcctgg
      601 ctgctaaatc gccaccgttg tcgccaccga caccatcaca caaaacagct aaaatcccgc
      661 catcaacttc caccgtttcg acagcatctt cattaaattt tttttgtgaa ccagggcttg
      721 tgaaccgaac gtaattaaac cccatcaggg gacctcgtaa aaattattta aatttttgaa
      781 tatatgtttt tatcaaaaat aaggaattct aaccttatct gctctaaaaa gcagatacag
      841 taccgggtga taatagtcaa aatacttaat ttgtttagca atcaccataa gattatattt
      901 gcaggcgatt tttgattcat aaatcatttc agaatataac tttatataaa ttcttttgat
      961 caaaatttga atcattttgt gttctttatt gcgagaatgg cggaattggt agacgcgcta
     1021 gacttaggat ctagtaccga gaggtgtcgg ggttcgagtc cccgttctcg cactttcata
     1081 tcatcccaat taaattattt gaggttagtt tttatatgga atataatgta gtagaattaa
     1141 attcttcaga aaaagaagtc gaaattaaac ttcagtacga tgagattcaa aaagaaattg
     1201 aagaagaagt aagaaaacaa tcaaagaaaa tccagattcc gggttttaga aaaggtaaag
     1261 tacctgcttc gatgctgaaa aaaatgtatg gcgatgcact tgaatatgaa gcttcggaaa
     1321 aaatagcaaa ttcattcttc tggaaagttg ccgaagaaaa tcaattaaaa ccaatcggtc
     1381 agccgacaat gacagactta aaatttgaac ctgaaaagga attacttttt aaagtcaaat
     1441 atgaaacaat cccggtgctt aatgtaaaag attataaagc tatttctttt gatgtacctg
     1501 aacttatt
//
LOCUS       DASWBY010000475         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_432903, whole genome shotgun sequence.
ACCESSION   DASWBY010000475 DASWBY010000000
VERSION     DASWBY010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432903"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VF870_08500"
     CDS             complement(<1..737)
                     /locus_tag="VF870_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9252268.1"
                     /translation="MKNLELKIKNYGSLRMVSFAKTLTLFLFFNQLISAQSVDSLVNE
                     ALLNNPKLKSLQDRIKASEYRSESVDYLPPPNLSVEFSQVPVSEIDIFNQSISNNFGL
                     SQMFPLGGKLDAMAEVENKNTLIEGNNFDAYKINLTARVKMSYFNLWLIDRKIEIQKG
                     NISLISDFAKAIEVSFYTNKISQADLLTVQSEIASNEAQILILEKQREAEVYKLNKYL
                     GRDLNSKDVFVLTEFKVDTLALNQEQL"
     gene            complement(739..>1725)
                     /locus_tag="VF870_08505"
     CDS             complement(739..>1725)
                     /locus_tag="VF870_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9252269.1"
                     /translation="SSPMNVQSAMNNNSSLGISNNRGGMSSMGSSNNAGITTQPNSGQ
                     NGFSLLTTEQNAITYLPISELADVNVLTGPPMISSENGMLRSIVFMNTRGRDMGNVMI
                     DAKQVIEDNLKLPAGYSYTWSGQYESKVRAQQTIQIIMPVVFLIIFVLLFLTLKDYME
                     AGVVMLSVPFALIGGMYMIYLLGYNFSVAVWVGFIALYGVAVETGVVMVVYLHEALDK
                     RIRAHKKGTRGPITKKDIYEATVEGSVLRLRPKLMTVGTSMIGLIPVMWATGTGSDVM
                     KPLTAPLIGGLLTSAVHVLVVTPILFAIMKERALRKGKLELSKMSDWMKEGE"
BASE COUNT          520 a          350 c          268 g          587 t
ORIGIN      
        1 tccagttgct cttggtttaa tgctaatgta tcaactttaa attccgtcaa tacaaaaaca
       61 tctttggaat tcagatctct tcccagatat ttgttcagct tgtaaacctc tgcttctctt
      121 tgtttctcaa gaattaaaat ctgagcttca tttgatgcta tctcgctttg aacagtgagt
      181 aaatccgcct ggcttatttt attagtataa aatgatactt caattgcttt agcaaaatca
      241 cttattagag aaatattacc tttttgtatt tcaatctttc tgtcgattag ccataaatta
      301 aaatatgaca tcttcacccg tgcggttaaa tttatcttat aagcgtcaaa attatttcct
      361 tcaattaaag tatttttgtt ttcgacttca gccattgcat ctaatttacc accaagcgga
      421 aacatttgag ataagccaaa attattagat atagattgat taaaaatatc aatttcactt
      481 accgggactt gcgaaaactc tactgataga tttggaggcg ggagataatc aacagattca
      541 cttctgtatt ctgatgcttt aattctgtct tgcaatgact tcaatttagg attattcaaa
      601 agcgcttcat taactaatga atcaacagac tgtgcactga tgagttggtt aaagaataaa
      661 aataaagtta atgttttagc aaacgatacc attcttagtg atccataatt tttaattttt
      721 aattctaaat tcttcatttt attctccctc cttcatccaa tcggacattt tagataattc
      781 taactttcct tttcttaatg cacgttcttt cataatcgca aaaagtatag gtgttacaac
      841 aagaacgtgt actgcggatg ttaataatcc tccaatcaat ggtgcggtca aaggcttcat
      901 tacatctgaa cctgttcctg ttgcccacat aactggaatc aatcctatca tagaagttcc
      961 tacagtcatc agtttcggcc ttagtctcaa caccgaacct tcaactgtgg cttcatagat
     1021 atcctttttt gttattggtc ctcttgtccc ttttttatga gcccgtattc ttttatcaag
     1081 agcttcgtgc aagtaaacaa ccatcacaac tcctgtttca acagcaacgc catacaaagc
     1141 gataaatcca acccatactg caactgaaaa attgtaaccg agaaggtaga tcatatacat
     1201 tccgccaatc agtgcaaatg gaactgatag catcacaaca ccagcttcca tataatcttt
     1261 caatgttaga aagagcagca caaagattat taagaacaca acaggcataa tgatttgaat
     1321 agtttgctgt gcccggactt tactttcata ttgtccgctc caggtataac tatatccggc
     1381 tggaagttta agattgtctt caatgacttg tttcgcatca atcataacat tacccatatc
     1441 tctaccccgt gtattcataa aaacgattga tcggagcatt ccgttttcgc tgcttatcat
     1501 tggtggtcct gtgagcacat tcacatctgc taattcggag atcgggagat aagtaattgc
     1561 attttgttca gttgtaagta atgaaaaacc attttggccg ctattgggtt gagtagtaat
     1621 acctgcattg tttgaacttc ccatggagga catcccacca cggttattac taattccgag
     1681 tgatgaatta ttattcattg cagactgaac attcatcgga cttga
//
LOCUS       DASWBY010000476         8013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_545519, whole genome shotgun sequence.
ACCESSION   DASWBY010000476 DASWBY010000000
VERSION     DASWBY010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545519"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3641
                     /locus_tag="VF870_08510"
     CDS             <1..3641
                     /locus_tag="VF870_08510"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX9252270.1"
                     /translation="SRRAITGSTQIFVTRGSFTISTSPDKWFFQQGKVVKLKINASDF
                     SDKPIQTDFRVIINYPDDPKSMRMKSQSDTLFAKTNEAGSAVVSFSPKQDRTGYYNYR
                     VIAFDEKEREIEASSSFYIGDYNNYYYQRTNAGLEIITDKDSYEKGDSLIAYVFLLNP
                     GQELLLTYETDKILSYKKLKPENNSFEIREKLTDKFSPSFNISISFIKDRMLYQTTKL
                     VGVLAKDKFLNITLNPSKETYKPGEQAEYEIIVKDYLGNPVKSTEISFGIIDESIYAI
                     KEDETQPIETFFYSPQYSYLPTYNSLQSGYFSSYSRPATYIDKNYFSYKEDDAKHKGK
                     LYGKITIEKEESIPDGLFVILTGERYYYTAKVDSIGNYEIKNIAKGNYQLFVSAGMGG
                     MILISDLKVSGEKKYNFTIKEEQKRQIENMIAQSVGGMREGDAVGNTFARRDAAPMTN
                     EMSVADGKMLKSESKDKSAYVQADVRTNFVDALIWKAHVVTDENGKAKVEFKIPDNLT
                     TWRTTVRGITKDTDVGQKVNKFISRKDLLVRMETPRFFRQDDEVVISTNVHNYLTTKK
                     ITKIHFEGKNLKLISSFVNSKDIINKAGGEAKDTYEISIPSQTELRIDWKVRITNPTG
                     EATLNVSALTDEESDAMEVKVPIIPNGIKVTNPVVLHYPQENVNETIEISIPDNIDIR
                     TAAFSFNADPSLAGTMLKALDDLAGYPYGCVEQTMSRFLPTIIVAKTFKEINVPLKSQ
                     TIEELPKYVDAGLKRLYDFQHSDGGWGWWTNDQTHPYMTAYVIYGLSLAREAGYDINE
                     NAFYNGLNNLESQITNAKADIDETTLSYMLYSLSTTLKNQSYDKSNYVEIINILLRKN
                     LKSYPLSLLAITLKNMNENQRAIEVAVRLKKQVNEEKSFAFWGGEEWHYRWQNDNVQG
                     TAFAVKANLNIEGNSDLIAKAVRWLLLKRQGYSWRSTQETSVVLFALTDYLKITKELD
                     PDFSVKIFLNDKEVFNKRFDKSDVFKQSETLKFSGSEINTLTKGNNKLRIEKSGKGIL
                     YLSGLSEYFTEDIKSVEHNNGFKVRREYYILETGEKDGHIIYFKKKFDGTVTSGQDIF
                     VKTFVESKSDNLEYFILENMMPSGFEVVKDMDKYFIDGENNYQNNYDYYYDYMPWRWQ
                     YADREYRDEKVAFFVTSCQSKMEFSYIIKAQIPGDYKIMPAQGYLMYYPELNGFSDVV
                     NIKVNDVK"
     gene            complement(3710..7315)
                     /locus_tag="VF870_08515"
     CDS             complement(3710..7315)
                     /locus_tag="VF870_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEX9252271.1"
                     /translation="MKHFYSFVCAVIISISFFSTSLFAQPEGISVVKTSSGYAINFSL
                     QPFEFTSVSAEGNEFTDINISGYGLTPDVGMPALPQLSFNLFISTDQQKPAVIINSVN
                     KSTQTLDKKIYPKQMYWSRERSLSERPFVINSDYYNSDGIEKQLVNISEPFVIGGVTG
                     VMVTIYPFAYNPKENKLTITNTAAFEIKADNNTESTNLKSNAFNEYLKKVLINYQPDA
                     SLQSMRYLIITAPIFESGLASFVTHKSANGFTVDLFNTTTTGTTTTAIKSFIQARYDN
                     AATRPEFVLLVGDVQHIPSWIGSGEGNPNTDLNYVQLAGSDKFADAFIGRFSVTTAQE
                     LQNAIAKSIYMENYIATLAKKNIFMASTDNYSITEGTHNYVINTHFQPAGWQNTKLYT
                     VTYNATTQQLIDALNANQRFAIYSGHGAETYWADGPVLNQSQVRALTNTVIYPFVYSF
                     ACITGSYQIAESFGETWIRTTNGGSTFYGSSVNSYWDEDDILERRLIDAMFNDDLTRV
                     TPMFDKAKFDLASYYGGVTPTVLRYMEMYNLMGDPSMPVERQIPPDTIPPDVITDLAV
                     GDATSNSLTLNWTAPYDSTFGGVTSYDLRYSLTPITNDADFNNAPQRLFTGISDSAGT
                     PRSFTIDELEFNTTYYFAAKALDMWNNKSVMSNVTIGLTLFPPASQLSVDSIHCVSVT
                     DTTFTENLLISNTSTQNSTLDYSIALTNSTFPDIVQIVLTGVNENFKGQIEYKKDFPS
                     NIKGYSFKGAGGPDAFGYKWKDSNDPNGPAYVWTDIAANPDAVLVTFPNGTLDDGYTN
                     ALPIGFDFKFYGNNYSNIYLSTNGFLSFAALTSSYYSNATIPDGAAPNNIIAPFWDDL
                     DGRTQGTVHLLREADKLTIQFTNWQKYNGTGSLTFQVVLKSNDRIMFYYNNLNATLTS
                     ATVGIENAAGTDGLQIAYEAAYLVNNLAVQIAADPEWLILNNYAGTIYNGNSVNIGLM
                     INTDGMDIGNYSMDLEITTNDPAHQLMVVPITMTIDNAVPVELVSFNAEIVDGDVVLN
                     WSTATETNNNGFQIERKVRSPQSLVGNWENKAFVSGNGTTTERSFYSYTEKNEKPGTY
                     SYRLKQIDFDGSFSYSSEVEIEVTGPKDFALYQNYPNPFNPSTTIKFALPVKTNLSLS
                     VYNTLGEKVAEIFNGEMEEGYHEMIFNASGLSSGIYFYKIESENFNSIKKMILLK"
     gene            complement(7366..>8013)
                     /locus_tag="VF870_08520"
     CDS             complement(7366..>8013)
                     /locus_tag="VF870_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9252272.1"
                     /translation="APIAAVYGFLKLFNDDSIKLTPEQRKDYENRSMIRLDGLLKMVN
                     DLLDISRMELKTVNREIKKVCLHEIISSILELFQVDIKNKELNVIFEKDNSAHCINAD
                     SDEISRLFTNLISNAIKYNREQGIININLYQADNYLVTEIKDSGIGLKPEEKNKLFTE
                     FFRAKNDKTKNIRGTGLGLSIVKRIVESYSGKIEVESDYGEGTTFKVFLPKIISN"
BASE COUNT         2678 a         1324 c         1452 g         2559 t
ORIGIN      
        1 cctcacgcag agcaataaca ggatcaaccc agatttttgt tacacgcgga tcatttacaa
       61 tttcaacatc accggataaa tggttttttc agcaaggcaa agttgtaaag ttgaaaataa
      121 acgcatcaga tttttctgat aaaccaattc aaacagattt tagagttata attaattatc
      181 ctgatgatcc gaaaagtatg cgaatgaaat cgcaaagtga tactttattc gcaaaaacaa
      241 acgaagctgg ttctgctgtt gttagttttt ctccaaaaca agatagaaca ggttattata
      301 attatcgtgt tatcgctttt gatgaaaagg aaagagaaat agaagcatca agctcattct
      361 atattggcga ttataacaat tattattatc agcggacaaa tgccggactt gaaattataa
      421 ctgataaaga ttcctacgaa aagggagact ctttaattgc ctatgtattt ttacttaacc
      481 cgggccagga attgctgctt acttatgaaa ctgataagat tttatcttac aaaaaattaa
      541 aacctgaaaa taatagtttt gaaatccgtg aaaagctcac agataaattt tcgccaagtt
      601 ttaatatttc tataagtttt ataaaagata gaatgcttta tcaaacaaca aagcttgttg
      661 gagttttagc taaagataaa ttcttaaata tcacactcaa tccgtctaaa gaaacttaca
      721 aacctggtga gcaagcagaa tatgagataa ttgtaaaaga ttatctcggc aatccggtta
      781 agagtacaga aattagtttt ggaattattg atgaaagcat ctacgcgatt aaggaagatg
      841 aaacccaacc gattgaaaca tttttctatt cacctcaata ttcatattta ccgacttaca
      901 attcattgca atcaggttac tttagctctt acagcagacc agcaacttac attgataaaa
      961 attacttttc ttataaagaa gatgatgcaa aacataaagg taaactttat ggtaagatca
     1021 caatcgaaaa agaagaatca attccggatg gcttgttcgt aatcctaacg ggagaaagat
     1081 attactatac tgcaaaagtc gattcaattg gaaattatga aataaagaat attgcaaaag
     1141 ggaactatca attatttgtt tctgcaggta tgggcggaat gatattaata agtgatctta
     1201 aagtatctgg cgaaaagaaa tacaacttca ctatcaaaga agagcagaag cggcaaattg
     1261 agaatatgat tgcccaaagt gttgggggaa tgcgagaagg cgatgccgtt gggaacacat
     1321 ttgcaagacg agatgctgct ccgatgacta atgaaatgtc agtagctgat ggcaaaatgt
     1381 tgaaaagcga atcgaaagat aaatccgctt atgtgcaggc agatgtgcga acaaactttg
     1441 ttgatgcttt aatctggaaa gctcacgttg ttactgatga gaatggtaaa gcaaaagttg
     1501 agtttaagat accggataac ttaacaacgt ggcgaacaac agttcgcgga attacaaaag
     1561 acacagatgt tggacaaaaa gttaacaagt ttatcagcag aaaagattta cttgtgcgaa
     1621 tggaaactcc acgtttcttt agacaggatg atgaagttgt aatctctaca aatgttcaca
     1681 attaccttac aacaaaaaag ataactaaaa tccactttga aggtaaaaac ttaaaactta
     1741 tttcttcatt tgttaattca aaggatatca taaataaagc aggtggtgaa gcaaaagata
     1801 cttatgaaat tagcatccca tctcaaacag aattaagaat agattggaag gttagaatta
     1861 caaacccaac gggagaagca acattaaacg tgtccgccct aactgacgaa gaatcagatg
     1921 caatggaagt taaagttcct ataattccaa atggaattaa agtcactaat cctgttgtgc
     1981 tgcattaccc acaggaaaat gttaatgaaa caattgaaat ttctattccc gataatatag
     2041 acattagaac agctgcgttt tcctttaatg cagatccttc actagcaggt acgatgttaa
     2101 aagctcttga tgatcttgca ggttatcctt acggatgtgt tgaacagacg atgagcaggt
     2161 ttcttcctac aataattgtt gctaagacat ttaaagaaat taatgtacct ctaaaatctc
     2221 aaaccattga agaacttcct aaatatgttg atgcaggatt aaagcggctt tatgattttc
     2281 agcattctga tggtggctgg ggctggtgga ccaacgatca gactcatcct tacatgacag
     2341 cgtatgttat ttacgggtta agtttagcgc gcgaagctgg ttatgacata aatgaaaatg
     2401 ctttttataa tggattgaac aatctggaaa gccagattac aaatgctaaa gccgatattg
     2461 atgaaacgac actatcttac atgctttact cattaagcac tactctaaaa aatcaaagtt
     2521 atgataagag taactatgtt gaaatcatta atattcttct tcgtaaaaat ttaaaatctt
     2581 atccgctctc tttactcgca attactttaa aaaacatgaa tgaaaaccaa agagcaattg
     2641 aagttgcagt aagattaaaa aaacaggtta atgaagaaaa atcatttgca ttctggggcg
     2701 gagaagaatg gcactacaga tggcaaaatg ataatgttca gggaacggct tttgcagtta
     2761 aagcaaactt aaatatagaa ggcaattcag atttaattgc taaagcagtt agatggctgc
     2821 ttcttaaaag gcaaggttat tcttggagat caactcagga aacttctgtt gttctgtttg
     2881 cattaaccga ttatctaaaa ataacaaaag aattagatcc tgatttttct gtcaaaattt
     2941 tcttaaatga taaagaagta tttaataaac gatttgataa atcagatgta tttaaacaat
     3001 ccgagactct aaagtttagt ggtagtgaaa taaatacttt gacgaaagga aataataaac
     3061 taagaatcga aaaatccggt aaaggaattt tgtatctatc gggtttgagc gaatatttta
     3121 cagaagatat aaaatcagtt gagcataata atggattcaa ggtaagacgc gaatattata
     3181 ttttagaaac aggcgaaaaa gacggtcaca ttatttactt taagaaaaag tttgatggga
     3241 ccgtaacaag cggacaggat atttttgtaa aaacttttgt tgaatcgaaa tctgataatc
     3301 tggaatattt tattcttgaa aatatgatgc catcaggatt tgaagttgtg aaggatatgg
     3361 ataaatattt tatcgatggt gaaaacaatt accagaataa ctacgattat tattacgatt
     3421 atatgccgtg gaggtggcaa tatgctgatc gcgaataccg tgatgaaaaa gttgctttct
     3481 ttgttacaag ctgccagagt aaaatggaat tctcatacat catcaaagcc caaatccctg
     3541 gcgattataa aattatgcct gcacagggtt atttaatgta ttatccggaa ttaaatggtt
     3601 ttagtgatgt ggtgaatatt aaagttaatg atgtgaaata gtctctctgc gactcagcgt
     3661 ctctgcggtg aagaaaatgt tttaccgcag aggcgcaaag acgctgagat tattttagaa
     3721 gtatcatctt ctttattgag ttaaaatttt cactttctat tttgtagaag taaatgcctg
     3781 atgataaacc ggaagcatta aatatcattt cgtgatagcc ttcttccatt tcgccgttaa
     3841 atatttctgc tactttttca ccaagtgtgt tgtaaacact taaacttaag tttgttttaa
     3901 ctggtaaagc aaacttaatt gttgtgctcg ggttaaatgg atttggataa ttttgataca
     3961 aagcaaaatc ttttggtcct gttacttcaa tttcaacttc gctactgtaa gagaagcttc
     4021 catcaaaatc aatttgcttt aatctgtaag aatatgttcc cggtttttca ttcttttccg
     4081 tatatgaata gaatgatctt tctgttgttg ttccattacc actaacaaat gctttgtttt
     4141 cccaattgcc gactagcgac tgcggactgc ggacttttct ttcgatctgg aatccattgt
     4201 tattcgtttc agttgcagtg ctccaattaa gtacaacatc accatcaact atttctgcat
     4261 tgaatgaaac aagttcaaca ggaactgcat tatcaattgt cattgttata ggtacaacca
     4321 ttaattgatg cgctggatca tttgtggtaa tttccagatc catagagtaa tttccaatat
     4381 ccattccatc tgtattaatc atcaatccga tattaactga attaccgtta taaatagtac
     4441 ctgcataatt attaagtatt aaccattccg gatcggcagc gatctgaact gctaagttat
     4501 ttacaagata tgcagcttca taagcaattt gaagtccatc agtaccggca gcattttcaa
     4561 ttccaacagt ggcgcttgtt aaggttgcat ttaaattatt gtaatagaac attattctgt
     4621 cattagattt taatacaacc tggaatgtta gtgaacctgt tccattatac ttttgccagt
     4681 ttgtaaactg aattgttaat ttgtctgctt cacgcagcag atgaactgtc ccttgtgtcc
     4741 ttccatcaag atcatcccag aaaggggcaa tgatattatt tggtgcagca ccatctggta
     4801 tagtagcatt tgaataataa gaagatgtaa gggcagcaaa gctcaaaaat ccattcgtgc
     4861 ttaagtaaat atttgaataa ttattaccgt aaaatttaaa gtcaaatcct attggaagag
     4921 catttgtgta accatcatct aaggtaccgt taggaaatgt aacaagaaca gcatcaggat
     4981 ttgctgcaat atccgtccaa acataagcgg gaccattagg atcattgctg tctttccatt
     5041 tatatccaaa ggcatcagga ccaccggcac ctttaaagct gtaacctttt atatttgatg
     5101 gaaaatcttt tttatactct atctgcccct taaagttttc attaacaccc gttaaaacta
     5161 tttgaacaat atccggaaaa gtactgtttg taagtgcgat tgaataatcg agtgtagagt
     5221 tttgtgtaga agtatttgat attaaaagat tctcagtaaa agttgtatcc gttacagaaa
     5281 cacaatggat tgagtcaacg cttagctgtg aagccggcgg aaagagtgtt aatcctatag
     5341 ttacattaga cataacagac ttattgttcc acatatccaa agcttttgca gcaaagtaat
     5401 aagttgtgtt aaattccaat tcatcaattg taaatgatct tggtgttcct gctgagtcag
     5461 aaattccggt aaataatctt tgcggtgcat tattaaaatc agcatcattt gtaattggag
     5521 ttaaagaata tcgtaaatcg taagatgtca ctccgccaaa agttgaatcg tatggtgctg
     5581 tccagtttaa tgtaagagaa tttgaagtag catcaccaac tgctaaatct gtaattacat
     5641 caggtggtat tgtatcagga ggaatttgtc tttcaaccgg cattgatgga tcacccatta
     5701 gattatacat ttccatataa cgcagaaccg ttggagtaac tccgccgtaa taagaagcaa
     5761 gatcaaactt tgctttatca aacattggag taacacgtgt taaatcatcg ttaaacattg
     5821 catcgatcaa tcttctttca agaatatcat cttcatccca gtaagaattt acagatgagc
     5881 cgtaaaaagt tgaaccgccg ttagttgtac ggatccaggt ttcaccaaaa gattctgcaa
     5941 tctgataaga tcccgtaatg catgcaaaag aataaacaaa tggatagata accgtgttgg
     6001 ttagtgctct aacctgtgat tgattaagca ctgggccatc agcccaatat gtttctgcgc
     6061 catgaccgga ataaatcgca aatcgttgat tagcgtttaa cgcatcaatt aattgctgtg
     6121 ttgtagcgtt gtaggttact gtataaagtt ttgtgttttg ccatccggca ggttgaaaat
     6181 gtgtgttgat aacataatta tgcgtgcctt ctgtaattga ataattatca gtcgatgcca
     6241 taaaaatatt tttctttgcc agagttgcga tataattttc catgtaaatg gattttgcaa
     6301 ttgcgttctg aagttcctgt gcagtggtaa ctgaaaatct tccgataaaa gcgtcagcaa
     6361 atttatctga tcccgcaagc tgtacataat ttaaatctgt atttggattt ccttcaccgg
     6421 aaccaatcca tgatggaata tgctgaacat ctccaaccag caaaacaaat tccggtcttg
     6481 tagctgcgtt atcatatctt gcctgaataa aagatttgat tgcagtagta gttgttcctg
     6541 ttgttgttgt attaaacaaa tccaccgtaa aaccatttgc agatttgtga gttacaaatg
     6601 atgctaaacc tgattcaaat attggagctg taattatcaa atatctcatc gattgcaagg
     6661 atgcatccgg ctgataatta attaatactt tttttaagta ttcgttaaag gcatttgatt
     6721 ttaagtttgt tgattcagta ttgttatcag cttttatttc aaatgctgca gtgtttgtaa
     6781 ttgttagttt gttttctttt ggattgtacg caaaaggata aatggtaacc attacacctg
     6841 ttactccacc gataacaaac ggctctgaga tattaaccag ctgtttttct attccgtctg
     6901 aattgtaata atcagaattg ataacaaatg gtcgttcgct aagcgatctt tcgcggctcc
     6961 agtacatctg ttttgggtaa atctttttat ctaatgtttg tgttgattta ttcacagagt
     7021 ttataatgac tgccggtttt tgctgatctg ttgaaataaa aagattaaag gagagttgag
     7081 gaagtgccgg cattccaaca tcgggagtta agccatagcc agaaatatta atatccgtaa
     7141 attcatttcc ttctgctgat acagaagtaa actcaaaagg ctgaagtgaa aaattaattg
     7201 cgtagccgct tgaagttttg actactgaaa tcccctcagg ttgagcaaaa agtgaagtgc
     7261 tgaaaaatga aattgaaatt atcacagcac acacaaaact ataaaagtgc ttcataatag
     7321 cccctgctaa atattaataa aaagcccctg aataaggttg gttaattaat tagaaataat
     7381 ctttggtaaa aatactttaa atgttgttcc ttcaccatag tcagattcaa cttctatttt
     7441 accggagtat gattcaacaa ttcttttaac tatggatagt cctaatcctg taccgcgtat
     7501 attcttcgtt ttatcgttct ttgcacggaa gaattctgta aacaatttat ttttttcttc
     7561 cggttttaaa ccaatgccgc tgtccttaat ttctgtaaca agatagttat cagcctggta
     7621 aagattgatg ttaataattc cttgttcacg gttatactta attgcattgc tgataagatt
     7681 agtaaacagt cgtgatattt catcactgtc ggcatttatg caatgtgccg agttatcttt
     7741 ctcaaaaatt acgtttagtt ctttgttctt aatatcaacc tggaataact cgagtattga
     7801 tgaaatgatc tcgtgcagac aaactttttt tatttcgcga ttaactgtct tcaattccat
     7861 tcgtgagata tcaagaagat cgtttaccat tttaagcaag ccgtccaatc ttatcatcga
     7921 acggttttca taatcttttc tttgttcggg agtaagtttg atcgaatcat cattaaataa
     7981 ttttaaaaat ccataaacgg cagcgatcgg tgc
//
LOCUS       DASWBY010000477         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_22207, whole genome shotgun sequence.
ACCESSION   DASWBY010000477 DASWBY010000000
VERSION     DASWBY010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22207"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1023)
                     /gene="obgE"
                     /locus_tag="VF870_08525"
     CDS             complement(34..1023)
                     /gene="obgE"
                     /locus_tag="VF870_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX9252273.1"
                     /translation="MFIDFVQIEVGSGKGGDGAVAFRREKYVPKGGPSGGNGGVGGSV
                     IFETQTNLSTLLDFRYKKKFFADNGKPGASSLKDGKNGADIIVKVPVGTIVKDAETEE
                     VLLDLSNPNEKVVFLKGGSGGKGNSNFATPTRRTPRFATNGKPGEQKKIILELKLIAD
                     VGLVGFPNAGKSTLISKISAAKPKIADYPFTTLEPNLGIVRYKDYQSFTVADIPGIIE
                     GAHQGKGLGIKFLRHIERTRILLFLIDITSGDYQKDFDTLYNELKKYSKKLVDKKIIV
                     SLSKADLIPEKELAKLSKVKIKKVKEPILIFSSATGFGLKTLEDKLWESLNKL"
     gene            complement(1023..>2368)
                     /locus_tag="VF870_08530"
     CDS             complement(1023..>2368)
                     /locus_tag="VF870_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252274.1"
                     /translation="KKILNITVTEFELIVNKIDRELFMLHQSKLVDNALFAIADYFSI
                     GGLNKSTVSVEAVENFLKEKNLTELLFKLKRAYTNTSKKKVDIDEIRKVLYSTLSIDS
                     PFVEDGNQAEVIADEKHFDNPLKEEFVNEEESAEVVLTEDENSSVDEILTNPASGSES
                     VQQLNEENNVESPSTQVETIDENSFEGVEEALAASEPEKFEINTNDVQSVEIPAKEID
                     ELEITEMELMVNDQNPVEENESPLIESVDEEDLLNSLTEDIEKDKGKDDEIIILDEKE
                     QEDLLNFYDNELEISIDDDVPVVKDKKSEIDENRTDEMDILEFEPEQNNDESTEDEVP
                     IEKIDFEKDLLGVDKSEESDVEEDSLIEPESTKSSADVFTYLSKKEIDRIIKNLFNSD
                     SEDFANTVEKMSLCKNYEEASAILDNIFKYARIKPHSRDAASLTNAVAKYFNTEK"
BASE COUNT          689 a          477 c          313 g          889 t
ORIGIN      
        1 tctttggccc tgaaattcaa aaaaaatact taattacagc ttatttaggc tttcccagag
       61 tttatcctct agcgttttta agccaaatcc tgtagcagat gagaaaatca atattggttc
      121 tttaactttt ttgattttaa cctttgaaag tttggcgagt tccttttccg gaataagatc
      181 agctttagaa agtgacacta ttattttttt atcgacaagt ttcttactgt attttttaag
      241 ttcattataa agagtatcaa aatctttctg ataatcccca gaagtgatat caattaagaa
      301 taaaagaatt cttgtccgct caatatgcct aagaaatttt attcctaatc cttttccttg
      361 atgcgcgccc tcaataatac caggaatatc agcgacggta aaactttgat aatctttata
      421 tctgacaata cctaaatttg gttcgagagt agtaaaagga taatcagcaa tttttggttt
      481 ggcagctgag attttagaaa ttagagttga tttgcctgca ttaggaaatc caaccaatcc
      541 aacatctgcg attaatttta attcaagtat tatttttttt tgttctccgg gtttaccatt
      601 tgtagcaaac cttggcgtcc gtctggttgg agttgcaaaa ttgctattac cctttcctcc
      661 tgaaccgcct tttaaaaaaa ctactttttc atttggatta gaaagatcta acaaaacttc
      721 ttcagtttcg gcatccttaa cgattgtacc aacaggtacc ttaacaatta tatctgcacc
      781 attcttgcca tcctttaaag atgaagcacc tggtttgccg ttatctgcaa aaaacttttt
      841 cttgtatctg aaatctaata atgttgataa gtttgtctgc gtttcaaaaa ttacacttcc
      901 gcctaccccg ccattacccc ccgatggacc gcctttggga acatattttt ctcttcgaaa
      961 agctacagcc ccatcaccac ctttacctga tccaacctca atctgaacaa aatcaataaa
     1021 cattattttt ccgtattaaa atattttgcg acggcattgg ttagtgaagc tgcatctcta
     1081 ctgtgaggtt ttattcttgc gtacttaaat atattatcca gaatagcaga agcctcttca
     1141 tagttcttac ataatgacat tttttctact gtgttagcga aatcttcact atcagaattg
     1201 aacagatttt taataattct atcaatttct ttttttgata aatacgtaaa gacgtcggca
     1261 ctgctttttg tactttctgg ctcaattaaa ctatcttctt ctacatcact ttcttcgctt
     1321 ttatcaactc caagcaaatc cttttcaaaa tctatttttt ctataggaac ttcatcttct
     1381 gttgattcat cattattctg ttcaggttca aactccagaa tatccatttc atcggttcta
     1441 ttttcatcta tttctgattt tttatctttt acgactggga catcatcatc gattgatatt
     1501 tccaattcat tatcataaaa gtttaataga tcttcctgct ctttttcatc caagataatt
     1561 atctcatcat ccttaccttt atctttttca atatcttcag tcaaactatt aagcaaatcc
     1621 tcttcatcaa ctgattctat taaaggtgat tcgttttctt caacgggatt ttgatcattc
     1681 accatcaatt ccatctcggt gatttctaat tcatcaattt cctttgcagg aatttcaaca
     1741 ctttggacat cattagtatt aatttcaaat ttttcaggtt cagatgctgc taaagcttct
     1801 tccactccct cgaatgaatt ttcgtcaatt gtttctactt gggtggaagg actctcaaca
     1861 ttattctctt catttaattg ttgaactgat tcagaccctg aagcagggtt tgttaaaatt
     1921 tcatctacag aactattttc gtcttctgtc aatacaactt cagccgattc ctcttcatta
     1981 acaaattctt cttttaaagg attatcaaaa tgtttttcat ctgctattac ctctgcctga
     2041 ttcccatctt caacaaaagg agaatcaata cttaaagttg agtacagaac ttttcttatt
     2101 tcatcaatat caactttctt tttagatgta ttagtgtaag cacgtttaag tttgaaaaga
     2161 agttctgtta gattcttttc tttcagaaaa ttttcaacag cttcaaccga tacggttgat
     2221 ttattcagac ctccaatact gaagtaatct gcaatcgcaa aaagagcgtt atcaacaagc
     2281 ttactttgat gaagcataaa taattcgcga tcaattttat taactatcag ctcaaactcg
     2341 gtaacagtaa tattcaaaat ctttttct
//
LOCUS       DASWBY010000478         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_246053, whole genome shotgun sequence.
ACCESSION   DASWBY010000478 DASWBY010000000
VERSION     DASWBY010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246053"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VF870_08535"
     CDS             complement(<1..1083)
                     /locus_tag="VF870_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252275.1"
                     /translation="MKKVLLISTIFLSLVFLLSCGEDFIDNPSGNKPPDTSVALFPDS
                     TIAPQPSRLKVAWWGDDPDGLIIGFYYTWDGTNWTFTDKNEILFSLQIGANDTTYLFK
                     VSAVDNGGNGVYDNQVFQNGINYGPEPFIDSNNDGVYNSGEKFFDIGLIDPSPADLAF
                     PLKNTAPTIEWNSLSTVPQESFPVMSFGWNVDDLDGTSTILKINIALNDTTNANNIVS
                     LDGSVRTITLRTKDFTSANPLMDILIEGLESNIFSEKLPGLKLDDYNTFYVQAVDVAG
                     ASSKFIALPDTGKTWYVKKPKGDIVIIDDYSTTDGAAAFYNSMMDSLGLSGKYDIYDY
                     LNQKPPYTSVTFLQTIKLFKYVLWYSD"
     gene            complement(1182..>2473)
                     /locus_tag="VF870_08540"
     CDS             complement(1182..>2473)
                     /locus_tag="VF870_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252276.1"
                     /translation="PLVTNIKRDIVEVASNQPVEISAKINDLDGTVSEARVYYSINSG
                     ARDSVQMNYSAADELYKGTIPGIAADSALVDFYIWAKDNQNLVSLTPVDTTKQNYFYL
                     VLNRPLTIKDAQYSPFGGGYSAYNGYRISLTGIVTADSSDIKGFGSTPLRIYLQDGSG
                     PWSGIQIGTAGTIGTQVLNLRRGDKVTVNGVIREDFDVTKIDTLTSISVVSSGNSLPE
                     AQVLTTGTIGTSGNNAIGKEEWESVLVQYNNVNITALSADGTSNFGEIYVSDGSGNTR
                     VELEDGNHSYQNGTRPERPTLVVLNGTFDAIKGVLYYSFSNYKLVPRKNDDFVNYTTD
                     VNDANILPAEYSISQNYPNPFNPSTTISYALPISEMVTIRIYNILGQVVSTLVNQNQS
                     AGIHTISFKADNLTSGIYFYSIQAGSFNQVKKMMLLK"
BASE COUNT          818 a          462 c          371 g          822 t
ORIGIN      
        1 atcactatac caaagaacat atttaaacaa tttaattgtt tgtaaaaaag ttacgcttgt
       61 atatggcggc ttttgattta aataatcata aatatcatat ttaccactta accccagact
      121 atccatcata gaattataaa atgcggctgc gccatcagta gttgaataat catctattat
      181 aacaatatca cctttaggtt tttttacata ccaggttttt cctgtatccg gtagtgcaat
      241 aaattttgaa gatgccccag ccacatcaac cgcctggaca taaaaggtat tataatcgtc
      301 cagctttaat ccgggtaatt tttcagaaaa gatatttgat tcaagtcctt caattaaaat
      361 atccattaat ggatttgctg atgtaaaatc ctttgttctt aaagttattg ttctaacact
      421 tccatccaga gaaacaatat tatttgcatt cgtggtatcg ttaagtgcaa tattaatttt
      481 aagtatagta cttgttccat caaggtcatc aacattccaa ccaaaagaca taacaggaaa
      541 tgattcttga ggcactgtgc tgagagaatt ccattctatt gttggggcag tattcttaag
      601 tggaaaagcc aaatctgcag gagaaggatc aatcaagcca atatcaaaaa acttttctcc
      661 actattataa acgccgtcat tattagaatc tatgaaaggt tccggtccgt aatttatacc
      721 attttgaaaa acctgattat cataaactcc atttccgccg ttatcaactg cagaaacttt
      781 aaataagtaa gttgtatcat ttgccccgat ttgaagtgaa aacaatattt catttttatc
      841 agtaaacgtc caatttgttc catcccaggt ataataaaaa ccgataatca aaccatcagg
      901 gtcatcaccc caccatgcaa cttttaatct gctgggctgt ggagcaattg tggaatccgg
      961 aaacaatgcc acactggtat caggcggttt gttgccggaa ggattatcaa taaaatcttc
     1021 tccacaagag agcagaaata ctaaagaaag aaatattgtg gatataagta aaactttttt
     1081 catttatcta aatgcaaaac aaataattaa taaatacttt aagtactatt ttattaacag
     1141 ttaattcctc ctcaattaaa ctgaggagga attaaagaaa actatttaag caacatcatt
     1201 tttttaactt gattaaagct gccagcctga atactgtaga aataaattcc ggaagttaag
     1261 ttatctgctt taaatgatat tgtatgaata cctgccgatt gattctgatt tacaagtgtt
     1321 gatacaactt gtcccaaaat attgtaaatc ctaatcgtta ccatttcaga tattggaagc
     1381 gcatacgaaa tagttgtaga tggattaaat ggattcggat aattttgcga tatagaatat
     1441 tctgctggaa gaatattcgc atcatttaca tcggttgtgt aattaacaaa atcatcattt
     1501 tttcttggaa ctaacttata attgctaaaa gaatagtata gaactccttt aatagcatca
     1561 aaagtaccat ttagaacaac caatgttggt ctttccggtc ttgttccatt ctgataggag
     1621 tggttaccat cttctaattc aactcttgta tttccggaac catcactaac atatatttct
     1681 ccaaaatttg atgttccatc tgcacttaaa gctgttatat ttacattatt atattgaaca
     1741 agtacactct cccattcttc ttttccaatc gcattatttc cagaagttcc tatagttccg
     1801 gttgttaata cttgtgcttc aggtaaagaa ttacctgatg atacaactga gattgatgtg
     1861 agagtatcaa tctttgtaac atcaaagtct tctcttataa ctccgttaac agttacttta
     1921 tcacctctgc gtaaattcaa cacttgagta ccaattgtac ctgctgttcc aatttgaata
     1981 ccactccaag gtcctgatcc atcttgcaaa tatattctca aaggtgtgct tccaaaacct
     2041 ttgatatctg atgaatctgc agttacaatt cctgttaaac ttatacgata accattgtac
     2101 gcactatatc ccccaccaaa tggactgtat tgcgcatctt taattgttaa cggacgattg
     2161 agcacaaggt aaaaataatt ttgcttagtg gtatcaaccg gagttaagct aactaaattc
     2221 tgattatctt ttgcccagat ataaaaatct actaaagcag aatcagcagc gattcccgga
     2281 attgttcctt tatataattc atccgctgca gaataattca tttgaacaga atctcttgca
     2341 cctgaattaa tcgagtagta aaccctagct tcagaaaccg ttccatcaag atcattaatt
     2401 tttgcagata tttcaacagg ttgatttgaa gcgacttcaa caatgtctct ttttatatta
     2461 gtaactaatg ggg
//
LOCUS       DASWBY010000479         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_318958, whole genome shotgun sequence.
ACCESSION   DASWBY010000479 DASWBY010000000
VERSION     DASWBY010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318958"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /gene="ribB"
                     /locus_tag="VF870_08545"
     CDS             complement(<1..780)
                     /gene="ribB"
                     /locus_tag="VF870_08545"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX9252277.1"
                     /translation="MFCSVAEAIEEIKNGKVIIVVDDEDRENEGDFICAAEYVTPDIV
                     NFMVTHGRGMLCVALNGKRLDELNLPLMVESNSALHGTQFTVTIDAIEGTTTGISAAD
                     RALTIKKLIDPKSTSKDFAKPGHIFPLRAFEEGVLRRAGHTEAAVDLCKLASLNPAGV
                     LCEILKENGEMARVPELFEIAKKYDLKIVTVQELIKHRIKNENLIEKISDIDFPTNHG
                     RFRLHLYKNLLDKKKQIALVKGQPDPEKPFLVRMHSECLTGD"
     gene            complement(790..1095)
                     /gene="yajC"
                     /locus_tag="VF870_08550"
     CDS             complement(790..1095)
                     /gene="yajC"
                     /locus_tag="VF870_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX9252278.1"
                     /translation="MDILIAMAPQGGEGGGGSLISTLIMFGAIFAIFYFMIIRPQQKR
                     AKEREKLLSNIEKGDKIITSSGVHATIVGIEEKTVLIEIAPNVKIKIERSGIGSVIK"
     gene            complement(1119..>1750)
                     /locus_tag="VF870_08555"
     CDS             complement(1119..>1750)
                     /locus_tag="VF870_08555"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX9252279.1"
                     /translation="WAVLNKEAFENSKALYGHKQFQFGIIQGSIYKDLRKKSVNDLLK
                     LDFDSYAIGGLAVGEPMEQMYELVDFTTDFMPDDRPRYLMGVGRPENILESISRGVDM
                     FDCVMPTRNARNANIFTWNGTLSMRNAKYKDDFTSLDENCNCYACRNYTRAYLRHLFI
                     AEEILALELASIHNLYFYLELVRQARKHIVGGDFFEWKNKTVNEISIKN"
BASE COUNT          516 a          365 c          259 g          610 t
ORIGIN      
        1 atcaccggtt aagcattctg agtgcatcct tacaagaaat ggtttttcag gatcgggttg
       61 acctttaact aaagcaattt gttttttctt atctaaaaga tttttgtaaa gatgaagtct
      121 gaatcttccg tgattagttg ggaaatctat atcagatatc ttttctatta gattttcatt
      181 tttaattctg tgtttaatca attcctgaac agttacaatt ttgagatcat actttttagc
      241 gatctcaaac agttcaggaa cacgagccat ctcaccgttt tcttttaata tttcgcataa
      301 aactccggcg ggatttaagc ttgctagttt gcacaaatca acagcagctt ctgtgtgccc
      361 ggctcttctt aaaacacctt cttcaaaagc acgtaacggg aatatatgtc ctggctttgc
      421 aaaatcttta gatgtggatt taggatcaat aagtttttta attgtaagtg cacgatcagc
      481 agcagagatt ccggttgtgg ttccttcaat tgcatctatt gtaacggtaa attgcgttcc
      541 gtgtaaagca gagttcgact caaccatcaa tggaagattt agctcatcca accgttttcc
      601 atttaaagca acgcacagca tacctctgcc gtgcgttacc ataaagttta caatatccgg
      661 agtaacatac tctgcagcac aaataaagtc gccttcgttt tcacgatctt catcatccac
      721 aacgataata actttaccgt tttttatttc ttcgattgct tctgcaacac tacaaaacat
      781 aaaatctctt tatttaatta cactgcctat tccagagcgt tcaattttaa ttttaacatt
      841 tggagcaatt tcgataagta cagttttttc ttcaatcccg actatcgtgg catgaactcc
      901 acttgaagta ataatcttat cacccttttc aatattagaa agaagttttt ctctttcctt
      961 tgctcttttt tgctgcggtc ttataatcat aaagtaaaag attgcgaaga ttgcaccgaa
     1021 cattattaaa gtacttatta aacttccgcc gccgccttca ccgccttgtg gtgccattgc
     1081 tattagtata tccaatttac tctcctttag tttttaattt agttcttaat tgatatttca
     1141 tttacagttt tatttttcca ctcaaaaaaa tcaccaccta caatatgctt gcgtgcttgt
     1201 ctaaccaatt caagataaaa atacaaatta tgaattgatg caagttcaag cgccaatatt
     1261 tcttcagcaa taaaaagatg tcttaaatat gcccgggtat aatttctgca agcataacaa
     1321 ttgcaatttt catctaatga agtgaaatca tctttgtact ttgcattccg cattgaaagt
     1381 gttccattcc acgtaaatat atttgcgttt cgtgcattac gtgtcggcat aacacaatca
     1441 aacatatcca caccgcgaga aatagattcc aaaatattct cgggtcttcc cacacccatt
     1501 aaatatcttg gtctgtcatc aggcataaaa tcagttgtaa aatcaacaag ctcgtacatt
     1561 tgttccatcg gttcaccaac tgccagtcca ccaattgcgt agctgtcaaa atctaatttc
     1621 aataaatcat ttacagattt ttttctaaga tctttataga tgctgccctg gattattcca
     1681 aactgaaact gcttatgtcc gtaaagtgct ttggaatttt caaatgcttc tttatttaat
     1741 accgcccaat
//
LOCUS       DASWBY010000480         8389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_616977, whole genome shotgun sequence.
ACCESSION   DASWBY010000480 DASWBY010000000
VERSION     DASWBY010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616977"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VF870_08560"
     CDS             <1..202
                     /locus_tag="VF870_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HEX9252280.1"
                     /translation="RYASLKGIMASKKKTITEKAAAQSPNYSEVIEMNLPASKQPGRI
                     LGTDSSAVAELVKLLREEAKVI"
     gene            211..1179
                     /locus_tag="VF870_08565"
     CDS             211..1179
                     /locus_tag="VF870_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX9252281.1"
                     /translation="MANKILAILEQREGLLKKVSFEAASVASRLAKELNLDVEAVVAG
                     AEVNNLNDISKYGITKVVHLKNPDFANYTSSGYTEAISNYAKEVNAVCLIVGNTSLGN
                     DLAPRLAVKLNSGCVMDCVNLQVESGDVIATRPIYAGKAFIKTKLSSDVKVFTIRPNV
                     FKAESSENGNASVETKDISNPNLKAKVVSFKKAEGKLDVAEADIIVSGGRGMKGPENF
                     NLIEALADSLGAAVGASRAVVDAGWRPHREQVGQTGKTVSPSLYIACGISGAIQHLAG
                     MSTSKYIVAINKDKDAPIFSVADYGIAGDVFEILPVLTEEIKKIRS"
     gene            1197..1784
                     /locus_tag="VF870_08570"
     CDS             1197..1784
                     /locus_tag="VF870_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX9252282.1"
                     /translation="MDKVQCEILGLSTNPSTGGAYAILLKEIEGTRRLPIIIGAFEAQ
                     AIALEMEGIKPPRPLTHDLLKTLVDNLGASVVEIIVSELKENTFYAKIILEASGLTNE
                     IDARPSDAIALAVRTYAPIFVNEAVMNSAAFIPSDETEIPGFETSDLESDKKPLSKEA
                     QLATLQDKLREALESEEFERAAKLRDEIKRLSQNN"
     gene            complement(1781..3256)
                     /locus_tag="VF870_08575"
     CDS             complement(1781..3256)
                     /locus_tag="VF870_08575"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEX9252283.1"
                     /translation="MKTNPKIPEELIYKIWEEKRFDSSLTTADGLSIEIIDCGVRNQD
                     EAGPDYRNARIKIGNITFSGDVEIDTLHSDWKAHGHNLNQRYNKVILHTVLSNDSSYP
                     FVVTQSGRKVPTLSLEEYLSSSIKQNIQQDLKAIQTEDEIKMPCSQLNHNVDRREKLS
                     YLKNLGLLRFRKKCEKNVERLKELVLLNELHIKEPKVFHDFHKEISSREYTQKDFEHL
                     ELWQQLYYEELFEALGYSKNKDIMLKLSRAADIKFFKSIEQINKEKIESILFHLSGLF
                     PDVIDISNEETSEYMRNSLDLWDSVKDKYDSGLLNKNEWNFFKLRPQNFPTIRIAAGA
                     RLIEKIIMHEQFIRIINIFKQYNDVNKLITKLRNEIIIKGEGYWAGHYNFNKETKTKL
                     NYFIGLGRADEIIINIILPIYSVYFEIHGNKDLSQKVLDLFLNYYQKEGNHLVDQVND
                     TLGLKNEKFRSVYYQGMIDLFRNYCIKKKCMECEIGKQVFN"
     gene            complement(3398..4198)
                     /gene="pyrF"
                     /locus_tag="VF870_08580"
     CDS             complement(3398..4198)
                     /gene="pyrF"
                     /locus_tag="VF870_08580"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX9252284.1"
                     /translation="MKSQEKILNKNAEGKFICVGLDTDVNKIPGFLQNDSDGVFKFNQ
                     AIIQATKDFTAAYKINFAFYERLGTKGIAELEKTRELIPDDILSIADAKRGDIGNTSE
                     MYAKSVYEHFKFDAVTLHPYMGKDSLTPFLDFYDKMNYVLVLTSNPGANDFEKLELID
                     GSLLYQNILKSVNEWNVKENCGIVFGATKLDELKNNINNFGNLSVLLPGVGAQGGSLE
                     DVSKVFKESKRKNYLVNVSRGIIYKSGKEDFAEAARDEIISLNKITSG"
     gene            complement(4207..5436)
                     /gene="rodA"
                     /locus_tag="VF870_08585"
     CDS             complement(4207..5436)
                     /gene="rodA"
                     /locus_tag="VF870_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX9252285.1"
                     /translation="MRIDYKLSDKFDFGIFIPAALLIAIGLTAIYSSTFNHPTMSGNF
                     DRQLVFSIAAFFIFFITYSLPTNSFRSITTPTYLFSLLLLVVVIVIGRKVSGARSWLD
                     LGPFGFQPSEFAKIGTVLAMSAYLSRKNTDIDSFKDILISLGIGLIPVMLILLEPDMG
                     TAIVFLGTILMLIFWKGISLFGLFIVLSPGFVAVAAIFGFYYFLASLIIVLVVLILFR
                     KDIFFSGSIFALNLAAGFFTDYLYNALSPHQQKRIQSFIDPNTDPLGAGYNTIQAKVA
                     IGSGGIFGKGFLQGNQTQLQYIPEQWTDFIYCVIGEEFGFIGSMIVLILFLYLFLRIL
                     KVASTTKDEFLSLTIIGILSVYIIHFLINIGMVVGILPVIGIPLPFVSYGGSSLLVNM
                     FMLGIIANIYRTRKNYT"
     gene            complement(5426..7144)
                     /gene="mrdA"
                     /locus_tag="VF870_08590"
                     /pseudo
     CDS             complement(5426..7144)
                     /gene="mrdA"
                     /locus_tag="VF870_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
     gene            complement(7237..7728)
                     /gene="mreD"
                     /locus_tag="VF870_08595"
     CDS             complement(7237..7728)
                     /gene="mreD"
                     /locus_tag="VF870_08595"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX9252286.1"
                     /translation="MIKPQYIISILLFFPLLLIQTTIVPLLAIDTVIPDLILILLVYY
                     SITQGQIYGSVLGFIYGFLFDIITGSLLGSAMIAKTMAGFTAGYFSSENKQDQYLIAY
                     NFALIVLLSSLIDSTINAFFTSINFTSNIFLLFFQYALLPAIYTAAIALMGMIFYPKR
                     KLF"
     gene            complement(7725..>8389)
                     /gene="mreC"
                     /locus_tag="VF870_08600"
     CDS             complement(7725..>8389)
                     /gene="mreC"
                     /locus_tag="VF870_08600"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX9252287.1"
                     /translation="IFNISNLKSENVNLRRTNAEMMLQLSRLREYAIVNDDLKGLLAL
                     KDSSAFPLIPATIVSKSLTKSQGTITLNAGKNQSVKVGMPVITDKGLVGIIYSISDNY
                     SIARTLTNIDLKITVKSERTRENAIMKWNGEWLVMVNVPKTFDLKKGDRIVTSEISSI
                     IPFPLPVGLVYEIGNVEKGIFNEIRVHPFVDFQKVENVFILGIVKSKEIDDLEFNLLS
                     GQ"
BASE COUNT         2795 a         1511 c         1319 g         2764 t
ORIGIN      
        1 aagatatgct tcattaaaag ggataatggc ttcaaagaaa aaaactataa cagaaaaagc
       61 tgcagcacaa tctccaaatt attctgaagt aattgaaatg aatcttcctg cttcaaaaca
      121 accgggaagg attttgggaa ccgacagcag tgcggtagct gaattagtta aattattaag
      181 agaagaagca aaggttatat aggtaaaatt atggcaaata aaattttagc aatcttagaa
      241 caaagagaag gtttacttaa aaaagtttca tttgaagcag caagtgttgc ttccagatta
      301 gcaaaagaat taaatcttga tgttgaagct gtagttgctg gagcggaggt gaacaatctt
      361 aatgatattt ctaaatatgg aataacaaaa gttgttcatt taaagaatcc tgattttgct
      421 aattatactt caagcggata cactgaagca attagtaatt atgcaaaaga agtaaatgcc
      481 gtttgtttga ttgttggaaa tacttctttg ggaaatgatc ttgcgccaag attggctgtt
      541 aagctaaact ctggctgcgt gatggattgt gtaaatcttc aggttgaatc gggtgatgtt
      601 attgcaaccc gacctatcta cgctggaaaa gcatttataa aaacgaaact atcctccgat
      661 gtaaaagttt tcactatcag accaaatgtt tttaaagccg aatcatctga aaatggaaat
      721 gcatctgttg aaactaaaga tatatcaaat ccaaatctta aagctaaagt tgtttcgttc
      781 aaaaaagctg aaggcaaatt agatgttgct gaagcagaca taattgtctc tggcggcaga
      841 ggaatgaaag gaccggaaaa tttcaatctt atcgaagcac ttgcagattc actcggcgct
      901 gctgttggtg cctctcgtgc agttgttgat gcgggttgga gacctcatcg tgaacaagtc
      961 ggccaaacag gaaaaaccgt ctccccttcc ctatatattg cttgcggaat atcaggggca
     1021 attcagcatc ttgcgggaat gtcaacatca aagtacatcg ttgcaatcaa taaagataaa
     1081 gatgcaccaa tttttagtgt tgctgattat ggaatcgcag gagatgtctt tgaaatttta
     1141 ccggtgctta ctgaagaaat aaaaaaaatt cgttcataag ttaacggact attaagttgg
     1201 ataaagttca gtgtgaaata ttaggtttat cgaccaaccc atctacaggt ggagcctatg
     1261 caattttatt aaaagaaatt gaaggcacac gcaggttgcc aataattatt ggggcattcg
     1321 aagctcaggc aatagctctt gaaatggaag gaataaaacc tccaagacct ttaacacacg
     1381 atttattaaa aactctggtt gataatcttg gtgccagtgt ggttgaaatt attgtgagtg
     1441 aactaaaaga aaatactttt tacgcgaaga taattctgga agcatccgga ttgacaaatg
     1501 aaattgatgc acggccaagt gatgcaatcg ctttggctgt gcgcacttat gctccaattt
     1561 ttgtaaatga agcagttatg aattctgctg catttatacc ttctgatgaa actgagattc
     1621 ccggatttga aacaagtgat ttggagtctg ataaaaaacc actatccaaa gaagcacaac
     1681 ttgcaacttt acaggataaa ttacgtgaag cattagaaag tgaagagttt gaacgtgctg
     1741 caaaattaag agatgaaatt aaaaggctat cacagaataa ttaattaaaa acctgtttac
     1801 cgatttcaca ttccatacat ttctttttta tgcaatagtt tctgaataga tctatcattc
     1861 cctgataata aacactacgg aatttttcat tctttaatcc gagtgtatca ttaacctgat
     1921 caacaagatg gttaccttcc ttctgataat aattaaggaa caggtctaaa actttttgtg
     1981 aaagatcttt attaccatga atttcaaaat aaacagaata aataggcaga ataatattta
     2041 ttataatctc gtctgctctg ccaagaccga taaaataatt taattttgtt tttgtttctt
     2101 tattaaaatt ataatgacca gcccaatatc cttctccttt aataattatt tcatttctaa
     2161 gttttgtgat cagtttattt acatcgttgt attgcttaaa aatattaata atacgaataa
     2221 actgttcgtg cataataatt ttttcaataa gtcttgcgcc ggcagcgatt ctgattgtcg
     2281 ggaaattttg tggtcgcagc ttaaaaaagt tccattcatt tttattaagt aatcccgaat
     2341 catacttatc ctttactgaa tcccataaat ccagactgtt tctcatatat tcagaagttt
     2401 cttcatttga aatgtctatt acatcaggga acaatccgct taaatgaaaa agaatactct
     2461 caattttttc tttattaatc tgctcaatag atttaaagaa ttttatatcc gccgctctgc
     2521 taagctttaa cattatatct ttattttttg agtagcctaa cgcctcaaat aattcttcat
     2581 agtacagctg ttgccacaac tctaaatgtt caaaatcttt ttgagtgtat tctcgtgaag
     2641 atatctcttt atgaaaatcg tgaaagactt ttggttcttt aatatgcaat tcatttaata
     2701 aaaccagttc tttaagacgc tctacatttt tttcacattt ttttctaaat ctaagcaaac
     2761 caagattttt tagataactt aatttctctc ttcgatcaac attatgattc agttgagagc
     2821 acggcatttt aatttcatcc tcggtttgaa ttgcctttaa atcctgttga atattttgtt
     2881 tgattgaaga ggatagatat tcttcaagtg aaagtgtcgg aacttttcta ccactttggg
     2941 ttacaacaaa cggataggat gaatcattag agagtactgt gtgaagaatt actttattgt
     3001 atcgctggtt aagattgtgt ccgtgtgctt tccaatcaga atgcaaagta tcgatttcaa
     3061 catcgcctga aaatgtaata tttccaattt taattctggc atttctataa tccgggccgg
     3121 cttcatcttg atttctaact ccgcaatcaa ttatctctat tgataagcca tcagcggtag
     3181 tgagagaaga atcaaatcgc ttttcctccc agattttata gataagttct tcaggaattt
     3241 taggattggt tttcaaatgt gcactctgac ttgaatttta taacttaaaa aatttctttt
     3301 tagatttcac atatcaaatc tatgatttta tcaaacataa ctaaagagaa tatttaagta
     3361 ccaaaatatt tactaaatat tttcaataat aaatccatca accagaagtg attttattca
     3421 acgaaattat ttcatcccgg gcagcttctg caaaatcttc tttaccgctt ttatatataa
     3481 ttccacgact aacatttact aaataatttt tgcgcttact ttctttaaaa actttactta
     3541 catcttccaa actaccgccc tgtgcgccaa cgccgggtaa aagtacagac agattaccaa
     3601 agttattaat attatttttt aactcatcca gcttagtagc accgaaaact attccgcaat
     3661 tctctttcac attccattca ttaacacttt ttaaaatatt ctgatataaa agacttccat
     3721 ctatcaattc taatttctca aaatcatttg caccagggtt tgaggttagc actaaaacat
     3781 aattcatctt atcataaaaa tctaaaaaag gcgttaaaga atctttgccc atgtatggat
     3841 gcaatgtaac agcatcaaac ttaaaatgtt cataaacaga ttttgcatac atttcagatg
     3901 tattgccaat atcaccgcgc ttagcatctg cgatagaaag tatatcatcc ggaatgagtt
     3961 ctctagtttt ttctaactcg gcaattccct ttgtacccaa gcgttcataa aatgcaaaat
     4021 taattttgta tgccgcggta aaatctttag ttgcttgtat tatagcctga ttaaatttga
     4081 aaacaccatc tgaatcattt tgtaaaaagc ccggaatctt atttacatcc gtatccaatc
     4141 ctacacaaat aaattttcct tcagcatttt tatttagaat tttttcttgt gatttcataa
     4201 tatttactat gtatagtttt ttctggtacg ataaatatta gctattatac ccaacataaa
     4261 catatttacc agtaatgaac ttccgccgta acttacaaac ggaagcggaa taccgataac
     4321 aggcaaaatc ccgacaacca ttccaatgtt tattaagaag tgaattatgt aaacagaaag
     4381 gatccctatt attgttagac ttagaaactc atctttggtt gtagatgcaa cttttaaaat
     4441 ccgtaaaaac aaataaagga ataatatcag tacaatcatc gaacctataa atccaaactc
     4501 ttctccaata acacaataaa taaaatcagt ccattgttca gggatgtatt gtaactgagt
     4561 ttgatttccc tgcagaaatc ctttcccgaa tattcctcct gagccaatag caactttagc
     4621 ttgtattgtg ttataacctg caccaagcgg atctgtattc ggatctataa aagattgaat
     4681 tcttttttgc tggtgcggac ttaatgcatt gtaaagataa tcggtaaaaa atcctgcagc
     4741 cagatttaat gcgaagatag agccgctaaa aaaaatatcc ttacgaaata aaataagtac
     4801 tactaaaact ataattagtg aagcgagaaa ataatagaaa ccaaaaattg ctgctaccgc
     4861 aacaaaacct ggtgagagaa ctatgaataa tccaaaaaga cttatgcctt tccagaatat
     4921 cagcattaat attgttccta aaaaaacaat agccgttccc atatcaggtt caagtaaaat
     4981 caacatcaca ggaattagcc ctattccgag tgaaattaaa atatctttaa atgaatctat
     5041 atcagtattt tttctactga gatatgcaga cattgcaagt acagtaccaa tctttgcaaa
     5101 ctcagatggt tgaaatccaa acggacctaa atccaaccag cttcttgcgc ctgaaacttt
     5161 tcttccaatt actataacaa caaccaaaag cagaagcgag aataaataag tcggcgttgt
     5221 gattgatctg aaagaatttg taggaagtga atatgtaata aagaaaataa aaaatgctgc
     5281 gatactaaat actaattgcc gatcgaagtt tccgctcatt gtaggatggt taaatgtaga
     5341 actataaatg gcagttaaac ctatcgcaat caaaagagcc gccggtataa aaattccaaa
     5401 atcaaattta tcgctaagtt tataatcaat cctcaaaatt tgctccagca atggtttgat
     5461 tattatccgt tttgatttct ttatttttaa gtgtgtcttt tttcagatat gcttcaatca
     5521 tagcttttgc aaccggtgcc gcgtaagttc cgccaaaccc aacattttca ataactaccg
     5581 caattgcaat tttgggatct tctgcgggtg catagcaaac aaataaagca tgatctttac
     5641 cgtgcggatt ttgtgcagtt ccggtttttc cggcaatatt aatatcgggt atttttatat
     5701 gagttgctgt accggcgccg ttaactacaa gaaacattcc ttcttttaca atatcaaaaa
     5761 tttcctgttt aattcctgtt tttatttcag ggaacttgta aggaacaatc tgcttagttt
     5821 tatcatcaag atatcctttt acaatatgcg gctgaaatgt agagccgttg tttgcaatta
     5881 atgctacata ttgtgcaagt tgcaccggcg taacgctaac ttaaccctgc ccgattccta
     5941 aactggccat aatacttctt ggccattttt caccatacat tttaacataa taatcttcac
     6001 cgggtataaa gccggcagct tcttcgccga tatctattcc tgttttagca ttaaagccaa
     6061 atttaagagc gtattctttc cacttatcca gaccaatctt gtaaattaaa tggtagaaaa
     6121 aactgttaca ggatttttca attgcgtgca ctacattaat agttccatga ttaccatgac
     6181 atttaaagaa tctgccaaaa gtaaatccgc cgccgcagtt tattgtggtt gatggagtaa
     6241 taattcccat atccaaagcc gctattgcag caagcatttt aaaagttgaa cctgggggtt
     6301 ttaaagacat agttgcacga ttgaaagatg gtttatcagg attgttgtac aactcctgca
     6361 aaaactctct ggatgttaca tatgaaaact ggttaagatc ataatcaggt gcactgacta
     6421 acgctaaaat ctcaccggtt tttggttcaa ttgcaacaac agctccacgt cttccaatca
     6481 tttgttgttc agctaccttt tgaacatcag catcgtagga tagaacaaga tcttttcctt
     6541 taatcgaatt tttatcttcg gctccatctt tgtatctgcc aatttccttg cgctttgaat
     6601 ttacgagtac ataattaaat cctttgatac cgcgtaaatc tttttcatac tgtttttcaa
     6661 taccattgtg cccaacataa tcacccggca cataataatc gttttcttta cttaattgat
     6721 taggtgaaat ctcttttgtg tatccaaacg agtgtgaacc cttaatacca taaggataac
     6781 cgcgctgcat ttcaacaata taatctactc ccggtaaatg ctcctgattt tcttctatcc
     6841 aagaaactat tccgaactca attccacgct ttattcttat aggaatatac ttagaataaa
     6901 ttctgttctt ttttagaatc gaagctacaa aaccagaatc tgctccaagt attttatcca
     6961 ttaacggatt taatttctga tcataatctg caggagttat tcttaacgtg taggcaggta
     7021 aattatctac cattactttt aaatttctat cataaaaaac tccgcgaaat ggaatttgct
     7081 ctattgcttt tatactgtta ttcgcagact tttcttcata agcctcctga tttaagattt
     7141 gcatttgaaa cagtctgaac gacaacacac caaatagaat tataacaaat gcatatataa
     7201 aagttcttct gcttattgaa gcaaaatttt cagatttcaa aacaatttcc tcttaggata
     7261 aaatatcatt cccattagtg ctatggccgc agtataaata gcgggtagta aagcgtactg
     7321 aaagaagagc agaaatatat ttgaagtaaa atttatcgaa gtaaaaaatg cattaattgt
     7381 tgaatctatt aaggatgaga gaagtacgat taatgcaaaa ttgtaagcaa tcaaatattg
     7441 atcttgttta ttttcagatg aaaaatatcc ggcagtaaat cctgccattg ttttagcgat
     7501 cattgcactt cctaataaac tgcccgtaat tatatcaaac aaaaaaccat aaataaatcc
     7561 caaaacagat ccataaatct ggccctgagt aattgaataa taaacgagta gaattaaaat
     7621 caaatcgggt attacagtat cgatcgctag cagaggtact atagttgtct gaattaatag
     7681 caacggaaag aaaagcagaa tagatataat atactgtggt tttatcattg cccgctcaat
     7741 aagttaaatt ccagatcatc aatctctttg cttttaacta ttcccaggat aaaaacattt
     7801 tcaacttttt ggaaatctac aaacggatga actctaattt cgttaaatat tcctttttct
     7861 acatttccta tttcatatac caatccgaca ggcaatggaa aaggaattat tgaactaatt
     7921 tctgaagtaa caatcctatc acctttctta agatcaaacg tttttggaac attaaccata
     7981 acaagccatt caccattcca tttcattatt gcattttctc ttgtgcgttc actttttaca
     8041 gttattttta aatctatatt agttaaggtt cttgcaatgg aataattatc tgatattgaa
     8101 taaataattc caactaaacc tttgtctgta ataacaggca tcccgacctt tacagattga
     8161 tttttgccgg cattaagagt gatagtacct tgagattttg ttaatgattt tgaaactatt
     8221 gttgcaggaa taagcgggaa tgcagaagaa tcttttaatg caagcaaacc tttaagatca
     8281 tcatttacta ttgcatattc acgcagtctg cttaattgca gcatcatctc cgcattcgtt
     8341 ctgcgcagat ttacattttc actttttaga ttactgatat tgaaaatat
//
LOCUS       DASWBY010000481         7245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_433133, whole genome shotgun sequence.
ACCESSION   DASWBY010000481 DASWBY010000000
VERSION     DASWBY010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433133"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /gene="ligA"
                     /locus_tag="VF870_08605"
     CDS             complement(<1..1468)
                     /gene="ligA"
                     /locus_tag="VF870_08605"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX9252288.1"
                     /translation="MSTSIQKKIEQIREEILKHDYNYYVLVEPVISDEEYDKLIKELE
                     KLESENPHLITPDSPTQRVGKDLTKEFNPVKHKVSMLSLANTYDEQDLFEFDRRVKES
                     LRESEKVEYVVELKIDGASVSINYIDGFLKTAATRGDGTVGEEITNNIKTIRSIPLKI
                     DLSKAKKYKLNNFEVRGEVYMRIADFEKLNKERETNGEKLFANPRNSTAGTLKLQDPK
                     IVSARKLNVFLYSLISLDEEFESQEENLKLLSKLGFTVNPQYKVCKSIDEVLKVCSQF
                     EESRHKLEYEIDGAVIKVNSMRQQKILGSIAKSPRWAVAYKFKAKQAFTKLNAITWQV
                     GRTGAITPVAELEPVKLAGSTISRATLHNFDEIQRKDIRAGDTVVIEKGGDVIPKVVS
                     VVLSERTDKSKGTFPPDKCPVCNSKLFKPENEVAFYCQNPECLAQIKGRIIHFASRGA
                     MDIEGLGDALIDLFVDKGFITHFSDIYKLKNKREELIQI"
     gene            complement(1472..2368)
                     /locus_tag="VF870_08610"
     CDS             complement(1472..2368)
                     /locus_tag="VF870_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX9252289.1"
                     /translation="MLDKFADELRQQRESAGLTLQQMATKTRIDLKFLEAIDQGNFSF
                     LPDLYVKAFVKQYAKTIGLDENITLKKFEAAREGMEYDPNPPAPVEEIKKEEAPKVEP
                     PKTESPRPAVKSTSPLKSYVDDQNQKSNDDEGKANKQLMIFGLGGVGLIIIAALLYFF
                     VFNKSDKIIVEETPIEEVIEQGNKRYVEEEPVEQITSDSSFVPLSGDSLYLTFYAKES
                     SWIFVVLDNNRTQEFTLSPNSKFSVAASREFKATVGNSGGVTLHLNNQPVDFTGRPGS
                     VKYFKLNKDGIVYLNTPPKIEQ"
     gene            complement(2387..3835)
                     /gene="rsmB"
                     /locus_tag="VF870_08615"
     CDS             complement(2387..3835)
                     /gene="rsmB"
                     /locus_tag="VF870_08615"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX9252290.1"
                     /translation="MEENTDQNLEQKIEDKNEHDEQSADQKNTGHLEEHKVIDLYSGV
                     RGLAVKILNRVERTDAYLEKLLDNELRNAELNGPDKALLYEIVHGVTRWQGRLDWILN
                     GFYKGQFSKAIPNLKNGLRVALYQILFLDRIPDHAAVNEAVEFVKKLQGQKPADLTNA
                     ILRNIIRNKNAIRYPDPNEDIIGYLTAYYSHPAWMVKRYLERFGREETEKLLTANNEK
                     PYLTLRVNALKTTPEEFKSLLGQVNLKYRPGKFLTEFVQLLNLTNITAWDYYTKGYFN
                     IQDESAGLAVRLLDVHPGMDVLDMCSAPGGKTAYLGSLMKNTGKITALDRYEGRLNLV
                     RKNNDRLGLTCVTTIESDALEFESKQFDRVLADVPCSGSGTLSKKPDIKWKKDIFDLR
                     RMTEMQLKLITKAAALVKPGGVLVYSTCSIEPEENFEVVKKFLESHPNFKFESAKGKV
                     ADTLVDENGCIQTLPQKHQMDGAFAAKLIRIE"
     gene            complement(3836..5092)
                     /locus_tag="VF870_08620"
     CDS             complement(3836..5092)
                     /locus_tag="VF870_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HEX9252291.1"
                     /translation="MQICIFEDINFSNFEPMVFSRPVYDLLCGIDSLKEKIIRSYGDV
                     KVSLHCRPYLADIVQHQNPNYLVNKIEDSECLFINGRIIAPENLSEILPLLPAEDKVF
                     VNEETVIAAYLSGKHLQNKINYLKDLFSVNDFNGLPIKILDIKCANYLWDLINQNGKQ
                     IVEDYSYLTQKADKKNYINDVEDYVHILNKANVLIGKDVTIKPGVVLDAASGPIIISD
                     EAFIYPNAVIEGPCFIGESSKIKSGATIYENVSIGKTCKVGGEVEQSLFMDYSNKQHA
                     GFIGHAYIGSWVNLGADTNNSDLKNNYSKIKIKLSNKEVNTGSQFLGLMIGDHSKSAI
                     NTMFNTGTVVGFSCNIFAPGFPEKYVPSFTWGGDSSSTVYDIKKAIETAKIVMSRRKI
                     DFETKDELMFDTIFELTKNDREKRGY"
     gene            complement(5175..>7245)
                     /locus_tag="VF870_08625"
     CDS             complement(5175..>7245)
                     /locus_tag="VF870_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9252292.1"
                     /translation="VEQTIIVAFLLVVLIIFLFLRDWRTTLIPMLVIPISLIGSFFIM
                     YIADFSINMLTLLGIVLAIGMVVDDAIVVLENIYTKIENKMDPIEAGKKGSAEIFFAV
                     ISTTVALASVFMPVIFLQGLTGRLFREFGIVVAGAVIISSFVALTLTPMLSAKILKRR
                     EHHNWFYNLTEPFFVAMNATYKRQLTYFISHRWLAFPVTAASMIIIYVLFATLPTELA
                     PLEDRSRLSITATAPEGATFEYMDRYIDEVVNVLNEKVPEREGIISVTSPGFGASSSV
                     NSGFINLILKQPEFRKKSQQQIADEITPLVNSLSGARTFITQQQTIGGGRRGGLPVQF
                     VLQAPNFQLLEEFLPKFIDETRNYPEFTIVDVNLKFNKPELKLSIDRQKARALGVSTI
                     DIAQTIQSAFSGQRFGYFIKDGKQYQVIGQFSRENRDAPIDLKSTYVKNSKGELIQLD
                     NVVFVNEQSAPPQLYRFNRYVSATVSAGLAPGVSLGEGIDVMYKIADKVLDEKFATAL
                     DGPSKDFKESSSSLLFAFILALGLIYLVLAAQFESFRDPFIILFTVPLALAGALFSLW
                     YFNQTLNIFSEIGIIMLIGLVTKNGILIVEFANQRKATGLTKNEAVISAATSRLRPIL
                     MTSLSTVLGILPIALALGAGSESRVSMGIAVVGGLILSTFLTLFIVPAIYSFFSRKTA
                     SVTNVME"
BASE COUNT         2234 a         1400 c         1112 g         2499 t
ORIGIN      
        1 ctatctgaat taattcttcg cgtttatttt ttagtttata aatatcagaa aagtgggtta
       61 taaatccttt atcaacaaag agatcaatca gtgcatcacc aagtccttca atatccatag
      121 ctccgcgtga tgcaaagtga attatgcgac ctttaatttg agctaaacat tccggattct
      181 gacaataaaa ggctacttca ttttccggtt taaataattt agaattacaa acagggcatt
      241 tatccggtgg aaatgttcct ttacttttat ctgtccgctc acttaaaaca accgaaacaa
      301 cttttggaat tacatcacca cccttttcaa taacaacagt atcacctgct ctaatatcct
      361 tacgctgtat ttcatcaaaa ttgtgtagtg ttgcacggct gatggttgaa cctgctaact
      421 ttaccggttc taattcagcc acgggagtta ttgcaccagt tcttccaacc tgccaggtta
      481 ttgcatttaa tttagtaaac gcctgctttg ctttaaactt atatgcaact gcccagcgag
      541 gagactttgc aatgctgcct aatatttttt gctggcgcat tgagtttact ttaataacag
      601 caccgtcaat ttcatattca agtttgtggc gactttcctc aaactggctg caaactttta
      661 atacttcatc aatactttta caaactttgt attgaggatt aactgtaaac ccaagcttac
      721 ttaataactt taaattttct tcctgtgatt caaactcttc atccaaacta ataagcgagt
      781 aaagaaaaac atttagcttt cttgcagata caattttggg atcctgtaat tttaatgtgc
      841 cagctgttga gttacgtgga ttggcaaaaa gtttttcgcc attcgtttcc cgttctttat
      901 ttaacttttc aaaatcggca atacgcatat aaacttcacc acggacttca aaattattaa
      961 gtttatattt tttcgcctta cttaaatcaa tttttaaagg aatagaacga attgttttga
     1021 tgttattagt tatctcttct ccaacagttc catcgcctct tgtagctgcc gtttttaaaa
     1081 atccatcaat ataattaatg cttacagagg caccatcaat ttttaattca acaacatatt
     1141 caaccttttc cgattcccgt aaactttctt taacccttct atcaaattca aataaatcct
     1201 gttcatcata agtattggca agacttaaca ttgatacttt atgttttacc ggattaaatt
     1261 cttttgtaag gtcttttcca accctttgag tcggcgaatc gggagtaatt aaatgcggat
     1321 tttcagattc aagtttttcc aattccttaa taagtttatc atactcttca tcacttatta
     1381 cgggttcaac taaaacataa taattataat catgttttaa tatttcctca cgaatctgtt
     1441 caattttctt ttgaatagaa gttgacattt actattgttc tattttagga ggtgtgttta
     1501 aataaactat tccgtcctta tttaatttaa aatatttaac cgaaccggga cgccctgtga
     1561 aatcaacagg ctgattattt aagtgcaaag taaccccgcc ggaattacca acagttgctt
     1621 taaattccct ggatgcggca acactaaatt ttgaattagg actgagagta aattcctgtg
     1681 tgcgattatt atccagcaca acaaaaatcc acgaagattc ttttgcataa aaggtcagat
     1741 acagactatc accagaaagc ggcacaaatg aactgtcgct ggttatttgt tcaacaggtt
     1801 cttcttcaac atatctctta ttcccttgtt caatcacttc ttcaattgga gtttcttcga
     1861 ctattatttt atcgctttta ttaaaaacaa aaaagtaaag cagggcagca ataataatta
     1921 atccaactcc gcccaacccg aagatcataa gctgtttatt tgcttttcct tcatcatcat
     1981 tggatttttg attttggtca tcaacatagg attttaatgg agaagttgat ttaactgctg
     2041 gtcttggaga ttctgtttta gggggttcaa ctttcggagc ttcttctttt tttatttctt
     2101 ctacaggcgc aggtggattt ggatcgtatt ccattccctc tcttgcggct tcaaattttt
     2161 tcaaggtaat attttcatca agaccgattg ttttggcgta ttgtttaaca aaagctttta
     2221 cataaagatc tggaagaaaa gaaaaattac cctgatcaat agcttcaaga aatttaagat
     2281 caattcttgt ttttgtagcc atttgttgaa gggttaagcc agcactttct ctttgttgtc
     2341 ttaattcatc tgcaaattta tcaagcataa taattcttat atattgttac tcaattctaa
     2401 ttaattttgc tgcaaaagct ccatccatct gatgcttttg cggaagtgtt tggatgcatc
     2461 cgttttcatc tactaaagta tcagcaactt ttccttttgc tgattcaaat ttaaaatttg
     2521 ggtgcgattc aagaaacttt tttaccactt caaaattttc ttcaggttca atagagcagg
     2581 tactatatac caatactccg cctggtttaa ccaatgcagc agctttggta attaatttta
     2641 gctgcatttc cgtcattctt cttaaatcaa agatatcttt tttccattta atatccggtt
     2701 ttttagaaag cgtacccgat ccgctgcaag gtacatcagc aagcacacga tcaaactgtt
     2761 ttgattcaaa ttctaatgcg tcactttcaa tagtagtaac acaagttaaa ccaagccgat
     2821 cattattttt acgaaccaga ttcaatcttc cctcatatcg gtccagtgcc gtaattttac
     2881 ctgtgttctt cattaaagaa ccaagatatg ccgttttacc tcctggcgcc gaacacatat
     2941 caagaacatc cataccggga tgcacatcta acaatcttac cgctaaacct gcgctttcat
     3001 cctgaatatt aaaatatcct tttgtataat aatcccaggc tgtaatattc gtcaggttca
     3061 atagttgtac aaactcagtc agaaattttc cggggcgata tttaagatta acctgtccca
     3121 aaagagattt aaattcttcg ggtgttgttt ttaaagcatt tactcttaat gtaagatatg
     3181 gtttttcatt gttcgcggtt aataattttt ctgtttcttc tctgccgaat ctctcgagat
     3241 agcgcttaac catccatgca ggatgtgaat aatatgctgt tagatatcct attatatcct
     3301 cattgggatc aggatatctg attgcatttt tatttctgat aatatttcta agtattgcat
     3361 ttgtaagatc ggccggtttt tgtccctgca gttttttaac aaactcaact gcttcattta
     3421 ctgctgcgtg atcaggaatt ctatccaaaa ataagatctg ataaagtgca acacgtaagc
     3481 cgtttttcag atttggaatt gcttttgaga actgtccttt ataaaatccg ttaagaatcc
     3541 aatccaacct gccctgccat cttgtaacac cgtgaacgat ttcatacaaa agtgctttat
     3601 caggcccatt tagttctgca tttcttaatt cattatctaa taacttttca agatatgcat
     3661 ctgttctctc tacacgatta agaattttaa ctgcaagtcc gcgaactccg gagtaaagat
     3721 ctataacctt atgctcttca agatgcccgg tatttttttg atcagcactt tgttcgtcgt
     3781 gttcattttt atcttctatt ttttgttcta aattttgatc agtgttctct tccaattaat
     3841 aacctctttt ttctctatca ttttttgtta gttcaaaaat tgtatcaaac atcaattcgt
     3901 ccttcgtttc aaaatcaatt tttcttctgc tcattacaat ttttgctgtt tcaattgcct
     3961 ttttaatatc ataaactgtt gatgaactgt ctccgcccca ggtaaaagat ggaacatatt
     4021 tttccggaaa tcctggtgca aatatattac aggagaatcc aacaacagta ccggtgttaa
     4081 acattgtatt aattgcactc ttagagtgat ccccaatcat caacccgaga aattgggagc
     4141 ctgtatttac ttctttattg cttagcttaa tttttatttt agaataatta ttttttaaat
     4201 cactgttgtt tgtatctgct ccaagattta cccaactgcc aatatatgcg tgaccgataa
     4261 atcctgcatg ctgtttattt gagtaatcca taaaaaggga ttgttcaact tcgccaccga
     4321 ctttacaagt ttttcctatc gaaacatttt cataaatggt tgcaccgctt tttattttag
     4381 acgattcgcc gataaagcaa ggtccttcaa taacagcatt tggataaata aatgcttcat
     4441 cagaaataat aattggtccg cttgcagcat ctaaaacaac tccgggttta attgtaacat
     4501 cttttcctat aagaacatta gccttattaa ggatatgaac ataatcttca acatcgttta
     4561 tataattttt tttatctgct ttttgagtta ggtaactata atcctcaaca atttgttttc
     4621 cgttctgatt gatcagatcc cataaatagt ttgcacattt aatatcaaga atttttatcg
     4681 gcaagccgtt aaaatcattt acactaaaaa gatctttgag ataatttatt ttattctgta
     4741 gatgcttacc ggataaatat gcagctataa ctgtttcttc atttacaaaa actttgtctt
     4801 cagcaggcag taatggaaga atctcggata aattttctgg agcaattatt cttccgttaa
     4861 taaacaaaca ctcagagtcc tcaattttat taactaaata gtttggattt tgatgctgca
     4921 ctatatccgc aaggtaaggg cggcaatgaa gtgaaacttt aacatcgccg taacttctaa
     4981 tgattttttc ttttagtgaa tctataccgc ataataaatc ataaacagga cgagaaaaaa
     5041 ccatgggctc aaaatttgag aagtttatgt cttcaaaaat gcagatttgc atatagtttt
     5101 ttatcctttt aaaaaattat aacagaattt aacatttttt gaggtaagat tctctaacga
     5161 aaaatgcgga aatactattc cattacgttt gttactgaag cagtttttct tgagaagaat
     5221 gaataaatag caggaacaat aaaaagggtt aaaaatgtag aaagaatcaa tccgccaaca
     5281 accgcaatac ccatagatac tctgctttcg gaacctgcac caagagctaa tgcaattggt
     5341 aaaatcccaa gtacagtaga aagacttgtc ataagaattg ggcgcaatct tgaggtggca
     5401 gcactgatta cagcctcatt ttttgttagt cctgttgcct ttctttgatt agcaaattca
     5461 acaatcaata ttccgttttt agttaccaaa cctataagca taataattcc aatctcgcta
     5521 aaaatgttca aagtttgatt gaaataccaa agtgaaaata atgcaccggc aagtgcaagc
     5581 ggtactgtaa acaaaattat aaatggatcg cgaaaacttt caaattgtgc tgcaagtact
     5641 aaatagatta atcctaatgc aagaataaaa gcaaaaagta aacttgatga actctcttta
     5701 aaatcttttg aaggaccatc caaagcggtt gcaaattttt catctaaaac tttatcagca
     5761 attttataca taacatcaat gccttcccct aaggatacac caggagctaa accagcagaa
     5821 actgttgcag atacataacg attaaatcta tacaattgag gaggagcgct ttgttcatta
     5881 acaaaaacca cattatctaa ttggattagt tcccccttag aattttttac gtatgtggat
     5941 tttagatcaa ttggtgcatc tctattttct cttgaaaact gtccgataac ctggtactgt
     6001 tttccatctt taataaagta accgaatcgc tgcccgctaa aagccgattg aatagtttgc
     6061 gctatatcaa tcgtagaaac tccaagtgct cttgcttttt gtctatcgat ggaaagtttt
     6121 aattccggtt tattaaattt tagattaaca tctacaattg taaactcagg ataatttctt
     6181 gtttcatcaa taaattttgg aagaaattct tccagtaatt gaaagttagg cgcttgcaaa
     6241 acaaattgta ccggcaaacc accgcgtctg cctccgccaa ttgtctgctg ctgagtgatg
     6301 aaagttcttg ccccactaag cgaattaact agcggggtaa tttcatctgc tatttgctgc
     6361 tgagattttt ttctaaactc aggttgcttt aaaattaaat ttataaatcc tgagttaact
     6421 gaacttgaag caccaaaacc aggtgatgta accgaaataa ttccctctct ctcaggaact
     6481 ttttcattca aaacatttac tacttcatca atatatctat ccatatattc aaaagttgcg
     6541 ccttccggtg ccgttgccgt aatagataat ctacttctgt cttcaagcgg ggcaagttct
     6601 gttggcagag ttgcaaaaag aacgtaaata ataatcatgg acgcggcagt cactggaaat
     6661 gctaaccacc gatgcgatat aaaataagtt aattgtctct tatatgtggc attcatagct
     6721 acaaagaatg gttctgtgag attgtaaaac caattatgat gttctcttct tttaagaatt
     6781 ttagcactga gcattggtgt taaagtaagt gcaacaaaag atgagattat aactgcaccc
     6841 gccactacaa ttccaaattc tctaaaaagt cttccggtca atccctgcag gaaaatcaca
     6901 ggcataaaaa cggatgcaag cgccactgtt gtagatataa cggcaaaaaa tatttcggcg
     6961 gatccttttt tgcctgcttc aatgggatcc attttatttt caatttttgt gtagatgttt
     7021 tctaacacaa ctattgcatc atccacaacc attccaatcg ccagcacaat accaagcagc
     7081 gttaacatat tgattgaaaa atctgctatg tacattataa aaaatgatcc tataagagaa
     7141 ataggaatta caagcattgg tataagagtt gttcgccaat cacgcagaaa taaaaatatt
     7201 ataagcacaa ccagcaggaa tgcaacaata atagtttgct caact
//
LOCUS       DASWBY010000482         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_93982, whole genome shotgun sequence.
ACCESSION   DASWBY010000482 DASWBY010000000
VERSION     DASWBY010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93982"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>1640
                     /locus_tag="VF870_08630"
     CDS             29..>1640
                     /locus_tag="VF870_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9252293.1"
                     /translation="MSDELIRKYKSKELLELQTASVALADKAFKILWFNKSFKKDVGT
                     GKIKGTSLKSLFNINTPDQKTIADSEKSFVVPLADKNKNIIITPLFSKSKKEVDAYFI
                     EMLPIVDSEYKQSEEKEKLRRNLVFQNELQSILVLLIKEKSVEKIAEEILIRCIDVTN
                     SDFGVIVFHHGEGVQKFLYSDNNNLIADKSEVEKSIKYNSSFINKWLEINKRPLLALN
                     HHNNIGYGLTQVLNCESLCISPCYFDNILHAKIFVGKKSGTFSSLDISDIEQFSILLS
                     FTISNIETRALNAALESRLLQAQKLETIGKLSSGMAHDFNNLLSSIFGSIHLLRSRVP
                     DSENVSKLIDNIENCSVRARDLTKGLLSFGKPTAKRKEIVMPNFLLSEIAKVVSQTFP
                     ANIILEEKVDTGLHNIIGNGTEIYQILLNLCVNAKESIEGIGKIVLSASNLIIDDTNI
                     INYPLLDRGKFVKFSVSDTGSGIDEENLSKIFDPYFSTKQKDTGSGLGLYVSYGIIKA
                     HKGHIDVTSNKGAGTTFDVYLPVFEPQKS"
BASE COUNT          591 a          254 c          284 g          511 t
ORIGIN      
        1 tacgaactta aacaagatta actactgaat gtctgatgaa ttaatcagaa aatataaatc
       61 aaaagagctt cttgaattac agacagcctc tgttgctctt gccgataaag catttaaaat
      121 tttatggttc aataaaagct ttaaaaaaga tgttggaacc ggaaaaatta aaggcacttc
      181 actcaagtct ctgttcaata taaatacacc agatcaaaaa actatcgcag attctgaaaa
      241 atcttttgtg gtaccgcttg cagataaaaa taaaaatatc ataattactc cgctcttttc
      301 aaaatcaaaa aaagaagttg acgcatattt catagagatg cttccgattg tggattcaga
      361 atataaacag agtgaagaga aggaaaaact cagaagaaat cttgtttttc aaaatgaact
      421 tcagagcatt cttgttttat taattaaaga aaaatcggtt gagaaaattg ctgaagaaat
      481 tctaataaga tgcatagatg ttacaaacag tgattttgga gttattgttt tccatcatgg
      541 agaaggtgtt caaaaattct tgtacagcga taacaataat ttaattgctg ataagagtga
      601 agttgaaaag agtataaaat ataattcttc ctttatcaac aaatggctgg aaattaataa
      661 acgtccgctt cttgcattaa accatcataa taatattggg tatggattaa cgcaggtgct
      721 taattgtgaa tcactttgta tttcaccttg ctattttgat aatatacttc atgcaaaaat
      781 atttgttggt aaaaaatcag ggacattttc ttcattagac attagtgata ttgaacaatt
      841 ctccatcctg ctttcattta caattagcaa tatcgaaaca cgggctctaa atgctgccct
      901 tgaatcaaga ttgctccaag cacaaaagct agagactatt ggtaaattga gcagtgggat
      961 ggctcacgat tttaacaacc tgctttcaag tatttttggc agcatacatt tactccgcag
     1021 ccgtgttcct gattcagaaa atgtaagtaa acttattgat aatattgaaa attgttcagt
     1081 aagagcaaga gatcttacaa agggtttgct ttcgtttggt aaaccaacag ctaagcgtaa
     1141 agaaattgta atgcctaatt ttcttttaag cgagattgca aaagttgtat cacaaacatt
     1201 tccagcaaat ataattcttg aagaaaaggt tgatacagga cttcataaca taattggcaa
     1261 tggaactgaa atctatcaaa tacttttaaa tttatgtgtt aacgctaaag aatcgattga
     1321 gggcataggg aaaatagttt taagtgcttc aaaccttata attgatgata ctaatattat
     1381 taattatccg ctcttggatc gaggcaagtt cgtaaaattt tctgtgagcg atacaggcag
     1441 cggaattgat gaagaaaatt tatccaaaat atttgatcca tatttttcca ctaagcaaaa
     1501 agatactggt tccggattgg gattatatgt ttcttacggt ataattaaag cacacaaagg
     1561 acacattgat gttacaagca ataaaggcgc agggaccacc tttgatgtct atctccccgt
     1621 ttttgagccg caaaagtcag
//
LOCUS       DASWBY010000483         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_319166, whole genome shotgun sequence.
ACCESSION   DASWBY010000483 DASWBY010000000
VERSION     DASWBY010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319166"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VF870_08635"
     CDS             complement(<1..927)
                     /locus_tag="VF870_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX9252294.1"
                     /translation="MKTISGNSLVKNSFYNIIGSIAPLFVALFAIPLLVHGLGEEKFG
                     ILSLAWVVIGYFSFFDFGIGRTLTKIIAENIGLDKFDDIPDLFWTALFLMLLISFVGT
                     ILLFFGTPFLVLNFLKISKEFQEESINTFYVLALSVPIVTTTAGLRGVLEAYQKFGTI
                     NIIRTILGVSTFAGPLLCLIFTDNLFIIVCFLTFIRLIIWYFYYRECIKLDSIRDIKK
                     NIRKTFIKPILKISTWMSISNLVGPILLYIDRFLIAALISATAVTFYTTPYEVITKIL
                     IIPGALTGVLFPAISSTYSTNPVLTKNLLSKSI"
     gene            complement(927..>1782)
                     /locus_tag="VF870_08640"
     CDS             complement(927..>1782)
                     /locus_tag="VF870_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9252295.1"
                     /translation="LMQIGGFSSGAVGLYFKKNLFAVSWGRSNYLASFFVVIIPLTFG
                     YLFYTRSKKLKLFLSIVLVFMFLGIILTLSRGGLLALFLALSLLFARTLKARSLIPFI
                     TVLLIIVTIILLNPLTYVIAEGMSSLETAGSVYSRLNFYEDTWKAFLKYPLTGVGFGN
                     LSFYATFILAKDASSSAHNIILGMLGETGLIGGIFFFSILGVVFIKVFKDYRSEEDHS
                     LKLLKWAFFSAICGGYIHSLVEPNFEGFQFSIIFWSVVGTFFNLNLLKTDQDFDILTD
                     PSTNEPRN"
BASE COUNT          667 a          303 c          266 g          546 t
ORIGIN      
        1 aatgcttttt gacaatagat tttttgtcaa aactggattt gtactgtatg tggatgatat
       61 agctggaaat aaaacaccag ttaatgcccc cggaattatt aatatttttg tgatcacttc
      121 gtaaggagtc gtgtaaaatg ttacagcagt tgcagatatt aatgcagcaa ttaaaaatct
      181 atcaatataa agcaatattg gtcctacaag attagatatg ctcatccagg tgctaatctt
      241 caagatgggt ttgataaatg tttttcggat attttttttt atatcacgaa tagagtcaag
      301 tttgatacat tccctataat agaagtacca gattattaat ctaataaaag ttagaaaaca
      361 aactataata aatagattgt cagtaaagat taaacagagc aatggaccag caaaagtgct
      421 tacccctagt attgttctaa ttatatttat tgtgccaaat ttttgatatg cttctagtac
      481 gcctctgaga cctgctgttg ttgtaactat aggtactgat aatgcaagga cgtaaaatgt
      541 atttatactt tcttcttgaa attcctttga tatctttaaa aaatttaaaa ccaaaaaagg
      601 ggtgccaaaa aacaataaaa tagtgccaac aaaactaatt aataacatta aaaacaaagc
      661 ggtccaaaat aggtctggaa tatcatcaaa cttatctaga cctatattct ctgctataat
      721 ttttgttagt gttcttccga taccaaaatc aaaaaaacta aagtacccaa taacaaccca
      781 ggctaatgat aatattccaa acttctcttc tcctagacca tgaaccaata acggaattgc
      841 aaacagtgca acaaataatg gtgcaatgct accaatgata ttatagaaag aattttttac
      901 aagtgaattg ccgctgatag ttttcattaa ttccgcggtt cgttcgtact tggatctgtt
      961 aaaatgtcaa aatcttgatc ggttttaagt aggttaagat taaaaaacgt tcccacaact
     1021 gaccagaata ttatcgaaaa ttggaatcct tcaaaatttg gttcaacaag cgagtgaata
     1081 taacctccgc atatagcgga aaaaaatgcc cattttaata atttaagcga atggtcttct
     1141 tcgcttcgat aatctttgaa taccttgata aacactactc ctaaaattga gaaaaagaaa
     1201 attccaccta tcagtccagt ttcgcccaac atacctaata ttatattatg tgcagatgac
     1261 gatgcatcct ttgctaatat aaaagttgcg taaaaactta aattcccgaa accaactcca
     1321 gtcaatggat atttaagaaa cgctttccag gtatcctcat aaaaatttaa tcttgagtaa
     1381 acagaaccgg ctgtttcaag actagacatt ccttctgcta ttacataagt aagaggattg
     1441 agaagaataa tcgttactat aattagtaaa actgtaataa aaggaattaa tgatcttgct
     1501 ttaagtgtac gagcaaacaa taaacttaag gcaaggaata aggctaacag accacccctt
     1561 gataaagtta gaattatacc caaaaacata aaaaccaata caattgacaa aaataatttt
     1621 aactttttcg atcttgtgta aaacaaatat ccaaatgtga gtggaattat cacaacaaag
     1681 aatgatgcta aataatttga tctgccccag cttactgcaa ataaattctt cttaaagtaa
     1741 agaccaacag ctcctgatga gaatccgcct atttgcatta aa
//
LOCUS       DASWBY010000484         6378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_433290, whole genome shotgun sequence.
ACCESSION   DASWBY010000484 DASWBY010000000
VERSION     DASWBY010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433290"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..612)
                     /locus_tag="VF870_08645"
     CDS             complement(97..612)
                     /locus_tag="VF870_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252296.1"
                     /translation="MLKTKEILRRKSQYSDPLSASVEDIFAEIENEIERKDEIEVLDR
                     NGKKVIVFNDPLYSREPRSTVNYSIFRGKWEFEPLEDIDENDSDKCAIQFKPLSDSAE
                     VIIAFKSIKDRVEFMNSIYASEEMKQVGSGMSLEKFANSKVMKTTGGLLGLTMAGYGL
                     YKIFAKKNEEK"
     gene            818..2899
                     /locus_tag="VF870_08650"
     CDS             818..2899
                     /locus_tag="VF870_08650"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEX9252297.1"
                     /translation="MHKRKIQSLILIFILFVSGSFFSGSTLSNKSSINPADTLTVIKP
                     ADQYQMENQLITSILTRYHFKEFEINDSLSSIIFNRYLDALDNGKNYFLASDIESFKS
                     QQFQLDDDLYKGDLQFYYDVFNVYLKRLNERIAFIDELLNHEFDYSVDENYEYDRDKS
                     DWAKNESELNELWRKRLKNDALTYKLNGKDWEFIQKTLKKRYKNFAHFLNQYNSEDVF
                     QLAMNTFTQAIDPHTNYFSPVTSDNFKIDMTLSLEGIGARLQTEDDYTKVAEIIAGGP
                     ADKSGLLKADDKIIGVAQGEDGEFEDIIGWRITDAVKLIRGAKGTLVRLQILKAGSDL
                     NAKPIEISLIRDKVKLEDQAAKGSVLEIMNEDKPFKIGVIDIPNFYSDFEGQRNGDSN
                     YKSTTKDVRKILDSLKTENVDGIIIDLRDDGGGSLQEAIELTGLFIKDGPVVQVKNSD
                     GNIDISDDPDPSIVYDGPLAVLVNRFSASASEIFTGAIQDYGRGLIIGEQTYGKGTVQ
                     NLIDLNRLSAKKNLNLGQVKLTIAKFYRVNGGSTQNLGVIPDIAFPSMIDPKDFGESS
                     EPSALPWDQIKSANFNKFSDLGKFIPELNKKHLQRISTNKDFQNLVEDINIYKENKKK
                     TFISLNEDIRKQEKQKEEKRKLEREGKTEDHVDTELLDDEVPIIKKDKNDYILNETAK
                     IISDYILMSVG"
     gene            complement(2896..3621)
                     /locus_tag="VF870_08655"
     CDS             complement(2896..3621)
                     /locus_tag="VF870_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252298.1"
                     /translation="MNLDISKITSCGSDWETNRYTFLQAMKNWQNDIRKNKLFPAIDF
                     AWQLQNKFNDILSENIESKYWLEREVKGAFIDDRLVILEKAHQISFQLDRLIDFVKWG
                     LNENIELLEEAEIIRQFVYDNIHITPCCSSDKYRGKGYILVPDNKIKAYKIYLYELSI
                     NWSVDEPVEYLDIELLRSIPFSMLKDSPYDLMKEFIEHSQNLYDPMVYVCETELDFPF
                     NETLFPVIKTKLLDSVNGLTPYI"
     gene            complement(3873..5405)
                     /locus_tag="VF870_08660"
     CDS             complement(3873..5405)
                     /locus_tag="VF870_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9252299.1"
                     /translation="MEFLKNLGIKEKNFGSSTGTKWNETTDQGELKIYSPATGEFIAS
                     VYQASESDLNKIMATAEEAFKYWRTVPAPKRGEIVRQIGNKLREHKKDLGSLVSFEMG
                     KSLQEGMGEVQEMIDICDFAVGQSRQLYGFTMQSERPNHRMYDQYHPLGIVCTISAFN
                     FPVAVWSWNAMLAAVCGDVNVWKPSSKVPLSAIACQNIIKDVLVENNLPEGIFTLIVG
                     KGSTIGEKILNDNRIPLISVTGSTNVGRHAGEVIAKRFGRAILELGGNNAIIMTPDVD
                     LKMAMPAVVFGAVGTAGQRCTTTRRLIVHDSIYDKVKDSLVKAYSGLKIGNPLDEKNH
                     VGPLIDKGAVKDFTNALSQVVAEGGKIIYGGNVLEGEAYKSGSYVVPAIVEAENHYKI
                     VQEETFAPILYLIKYSGDVKNAIAIQNGVVQGLSSSIFTNNMREAEMFLSVEGSDCGI
                     ANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKTYMRRQTNTINYGTALPLAQGIKF
                     EI"
     gene            complement(5426..5839)
                     /locus_tag="VF870_08665"
     CDS             complement(5426..5839)
                     /locus_tag="VF870_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9252300.1"
                     /translation="MSASTKLSTSVKALCFLASADKPKNSTEIASSVGINASKLRKLL
                     SMLGKAGIVKSDSGMLGGFSLAKKPGEIHLQEIYCAIEDRKAFYLNVNDDKLEKNSSS
                     EKINYFFLDLFSQIQVEIENKMTDITLKSILDKIK"
BASE COUNT         2167 a         1083 c          961 g         2167 t
ORIGIN      
        1 caaggtaata tttccaaaat attaagggac gttaatgttg aaacacacgc tgatgatgtt
       61 tttaatttat caatcgggct gaaatagatt tgtcttctat ttctcctcat ttttcttagc
      121 aaatatttta tacagtccgt aaccagccat agttaaaccg agtaatccgc cggtagtttt
      181 catcacttta gaattcgcaa acttttcaag gctcattccg ctaccgactt gtttcatttc
      241 ttcgctcgcg taaatagagt tcataaattc aactctatcc ttaatcgatt taaatgcaat
      301 gataacttcc gctgagtcac ttaatggttt aaactgaatt gcacatttat cagaatcgtt
      361 ctcatcaata tcttctaatg gttcaaattc ccatttaccc ctgaatatag aataattaac
      421 tgtacttctt ggttcacgag aatataatgg atcattgaac actattactt tttttccatt
      481 tcgatcaagt acttcaattt cgtctttacg ctcaatttca ttttcaattt ccgcaaaaat
      541 atcttcaacc gaagcactta ggggatctga atactgagat tttcttctta atatctcttt
      601 agtctttaac ataattttca ccaaattaac ttattttata tgcaaaattt ataaaaatta
      661 tcatccattt ctatcatcat ttctcattta ttgttaggga acacattggg attaagtata
      721 gtcttattaa cttgaaaata cagatgtaat tccatattta tatacagaaa acattaattt
      781 ttaatgagat atcaatctat tatcaagaaa gattaaaatg cacaaaagga agattcaaag
      841 tttaatttta atatttatat tatttgtctc gggcagtttt tttagcggaa gtacccttag
      901 caataaatca tccatcaatc ctgctgatac tttaacagta atcaaacccg cagatcaata
      961 tcagatggaa aatcagctta ttacttcaat actaaccaga tatcatttta aggagtttga
     1021 aataaatgat tctctatcaa gcataatttt taacagatat ttagatgcgc tggataacgg
     1081 aaagaactat tttcttgctt cagatattga atcattcaag tcgcagcaat ttcagttgga
     1141 tgatgatttg tataaaggtg atctgcaatt ttattatgat gtatttaacg tttatttaaa
     1201 aagattgaac gaaagaatag catttattga tgaactactt aatcatgaat ttgattattc
     1261 cgttgatgaa aattatgaat atgacagaga taaatctgat tgggcaaaaa acgaatctga
     1321 acttaatgaa ctttggcgta aacgattaaa aaacgatgct cttacttata aactaaatgg
     1381 caaggattgg gagtttattc agaaaacttt gaagaaaaga tataaaaact ttgctcattt
     1441 cttaaatcag tataactcgg aggatgtatt tcaacttgca atgaatacat tcactcaggc
     1501 gattgatccg catacaaatt atttttcacc tgtaacttct gataatttta aaattgatat
     1561 gaccttatcg ttagagggaa ttggtgcacg gttgcaaaca gaagatgatt atacaaaagt
     1621 tgctgaaatt attgctggtg gaccggcaga taaaagtggt ttacttaaag ccgatgataa
     1681 aattattggt gtagcccagg gcgaagatgg tgaatttgag gatattatcg gctggagaat
     1741 tacagatgca gttaaattga ttcgtggtgc aaagggaact ttagtaagac tacaaatttt
     1801 aaaagcaggt agtgatctta acgcaaaacc aattgagatt tcactaatac gtgataaagt
     1861 taaattggaa gatcaggctg ccaaaggcag tgttttggaa ataatgaatg aagataagcc
     1921 ttttaaaatt ggtgtaattg acatcccaaa tttttatagt gactttgaag gacagcgaaa
     1981 tggtgacagc aattataaaa gcacaacaaa agatgtaaga aaaattctgg attcattaaa
     2041 gactgaaaat gttgatggta taatcattga tttacgtgat gatggcggcg gttccctcca
     2101 ggaagctatc gaactaacag gtctgttcat taaagatggc cctgtagttc aggtaaaaaa
     2161 ctcagatggc aatatagata tttctgatga cccagatcct tcgatcgttt atgatgggcc
     2221 actagcagtt ttagtaaata gattcagtgc atccgcgtca gaaatattta caggtgcaat
     2281 tcaggattac ggcagaggtt taattattgg agagcaaact tatggtaaag gaactgttca
     2341 aaatcttatt gatcttaaca gattatctgc aaagaaaaat ttgaatttag gacaggttaa
     2401 attgactatt gctaaatttt atcgtgttaa cggaggcagc acacaaaatc ttggggtaat
     2461 tccggatatt gcatttccat caatgatcga tcctaaagat tttggagaaa gttctgaacc
     2521 cagtgcattg ccctgggatc aaattaaatc tgcaaacttt aataaatttt cggatcttgg
     2581 aaaatttata cctgaactga acaaaaaaca tttacagaga atttctacca ataaagattt
     2641 tcaaaattta gttgaagata taaatattta taaagagaat aagaaaaaaa catttatctc
     2701 cttaaatgaa gacataagaa aacaggaaaa acaaaaagag gaaaaaagaa agcttgaacg
     2761 ggaaggtaaa actgaagacc atgtggatac agaattatta gatgatgaag ttcccataat
     2821 aaaaaaagat aagaacgatt atatccttaa cgaaacagct aaaataattt ctgattatat
     2881 tttaatgagt gttggttaaa tatatggagt taatccatta acagaatcta aaagtttagt
     2941 tttaataacc gggaagagtg tttcattgaa tgggaaatct aattcggttt cacatacata
     3001 aaccattgga tcgtaaagat tttgagaatg ttctataaat tctttcatca aatcataagg
     3061 tgaatctttt aacatactaa atggtattga acgaagtaat tctatatcta aatattcaac
     3121 aggttcatcc acagaccagt taattgataa ttcgtataga taaattttgt aagctttaat
     3181 tttattatcc ggaacaagga tataaccttt gcctcggtat ttatcagaac tgcagcaagg
     3241 tgttatgtga atattatcat atacaaattg cctgattatt tctgcttctt caagcaattc
     3301 aatattttca ttcaaacccc atttaacaaa atcaattaac ctatcaagct gaaaactaat
     3361 ttgatgagct ttctcaagta tgactaacct gtcatcaata aatgcacctt taacctccct
     3421 ttccaaccag tactttgact cgatattttc agacaaaata tcattgaact tattttgaag
     3481 ctgccaggca aaatctatgg caggaaaaag tttgttcttt cttatatcat tttgccaatt
     3541 cttcatagct tgaagaaaag tgtaacgatt agtttcccaa tcagagccgc aagaagttat
     3601 ttttgatata tctaagttca tctttaataa ttttttatac cattaaagca aatgtcaggc
     3661 catcaaactc gcagataaag ttgtagtaca atcttgtcat ttacaaaatt tgtcgtaggt
     3721 tttttcttta gattctttga aaaaagaaga attatggaac aggaaaataa tgcatgtaat
     3781 attttctgat tatgaaatag gatataaaaa gaaagggaag taaaatttta caatcctact
     3841 tccctttttt tacattacaa aaacaagagg ccctaaattt cgaatttaat tccctgagct
     3901 aatggtaggg ctgtaccata gtttatagta ttagtttgtc ttctcatata agtcttccat
     3961 gcatctgaac ctgattctct gcctccaccg gtttcttttt caccaccaaa agcaccacca
     4021 atttctgcgc ctgaagttcc tatattgaca tttgcaattc cgcaatcaga tccttcaact
     4081 gataggaaca tctctgcctc tctcatatta ttagtaaaga ttgatgaaga taatccctga
     4141 acaacaccat tttgaattgc tattgcgttt ttaacatcac ctgaatattt aattaaatat
     4201 aagataggtg caaaagtttc ttcctgaaca atcttgtagt gattttcagc ctctacaatt
     4261 gcaggcacaa cgtaagaacc tgatttgtat gcttcacctt caagaacatt tccaccataa
     4321 ataattttac cgccttcagc aacaacctgg ctcaacgcat ttgtaaaatc tttaactgca
     4381 cctttatcta taagcggacc gacatgattt ttttcatcca atggattacc aatttttaat
     4441 ccgctgtaag cttttactaa cgaatcttta actttatcat aaattgaatc atgaacaatt
     4501 aatcttcttg ttgtagtaca tcgctgtccg gctgttccta ctgctccaaa aacaactgca
     4561 ggcattgcca ttttaagatc cacatccgga gtcataatta ttgcattgtt accaccaagt
     4621 tcaagaattg ctcttccgaa tctttttgca attacttcac ctgcatgtct tccaacattc
     4681 gttgagcctg ttactgaaat aagcggaatt ctattatcat tcaaaatctt ttcacctatt
     4741 gtagagcctt taccaactat taaagtgaat attccttcag gtaaattatt ttcaacaaga
     4801 acatctttaa taatattctg acatgcaatt gctgaaagcg gaacttttga agaaggtttc
     4861 caaacattaa catcaccgca aacggcagct aacattgcat tccatgacca aactgctaca
     4921 ggaaaattga atgcagaaat agtacaaaca attcctaacg gatgatattg atcatacatt
     4981 ctgtgattag gtctttccga ttgcattgtg aacccgtata attgtctgga ttggcctact
     5041 gcaaaatcgc agatatcaat catctcctgc acttctccca ttccctcctg caatgattta
     5101 cccatttcaa aagaaaccag agagccaaga tcttttttat gctcacgtag tttattacca
     5161 atctgtctga ctatttcacc acgttttggt gctggaacag ttctccaata tttaaatgct
     5221 tcttctgcag tcgccattat tttattaaga tcagattctg atgcctgata aactgatgca
     5281 ataaattctc ctgttgccgg agaataaatt ttcagttcac cctgatctgt tgtttcattc
     5341 cattttgttc ctgttgagga accaaaattc ttttctttga tgccaagatt ttttaagaat
     5401 tccattttgt aactcctttt aactgttatt taattttatc taatattgat tttaaggtaa
     5461 tatctgtcat tttattttcg atctcaacct gaatttgtga gaacaaatcg agaaagaagt
     5521 aatttatttt ttcagaggaa ctattctttt caagtttatc atcattaaca tttaaataaa
     5581 aagctttacg atcttcaatt gcacaataaa tttcctgtaa gtgaatttca ccaggtttct
     5641 tagcaagtga aaatccgcct aacattccgc tgtcactttt aacaattcct gctttaccaa
     5701 gcattgataa taacttacga agttttgacg cattaatccc gaccgaagaa gctatctctg
     5761 tgctattttt cggtttatct gctgatgcga gaaagcataa agcttttaca gaagtggata
     5821 atttagtcga agctgacata atttttataa ttgttacagt aagtgtaaca cttatttata
     5881 aaaaaaacaa ataaaaggtt tctaattaag atattattaa ttgacaattt ggataaataa
     5941 ctgccttact cattaaaaga atcaaaatat tctcggaata aaaattgtga gctatttctg
     6001 atacttaata tttttctcca taattaagtt taatcaagtc aaaattgtaa tatttgggga
     6061 aaataattag aaataagctt atgaaataat tcttattctt atttatattg tacttaagaa
     6121 ttttaatttt atgaataaaa acatcaaata tttctctact tctcaacgaa gctacttcgt
     6181 atccagcttc gaaaacgtat tctaatctcc ttctcctatt tctacctttt tctaattcat
     6241 acaatttttt aatcattatc tatttaggag tagaaaatga attctttaat aaccgacgat
     6301 aacaaaataa ataatacata ttccccctct attagcgccg atcaggtaat tccaactctt
     6361 tcaaaatata tgcttgct
//
LOCUS       DASWBY010000485         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_22622, whole genome shotgun sequence.
ACCESSION   DASWBY010000485 DASWBY010000000
VERSION     DASWBY010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22622"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..680
                     /locus_tag="VF870_08670"
     CDS             111..680
                     /locus_tag="VF870_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HEX9252301.1"
                     /translation="MIGNSLYINVTNRCNADCTFCRRKEDPFLNGYNLGMKKSEEPPA
                     EVYISEIGDPKKYDEIVFCGYGEPTIRWDVVKEVGRYVKANGGSTRINTNGHGNIINK
                     KDITPEMKDIIDVVSISFNTFDPKQYAELMRVSEDHFEEMKKFASLAKPYVKKVVMSV
                     VSLDEVEIEKSRKVVEEEIGVEFRVREYF"
     gene            730..>1838
                     /gene="dacB"
                     /locus_tag="VF870_08675"
     CDS             730..>1838
                     /gene="dacB"
                     /locus_tag="VF870_08675"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX9252302.1"
                     /translation="MKRILILSVIFFISAYPQSLKTKLDNVLKDKFFESCLVSIQVED
                     LTTNKTLYKDNEKMLLRPASNMKVITSSAGLLFLGPDYEFTTNLYHDGYVSNDTLYGD
                     IYVVGGCDPDFITQDLYTFIDTIKLLNISVINGNIYGDVSFKDSLYWGKGWMWDDNPS
                     SDAPYLSALNINDNCVEVFVQKSEYLDKPEVSLIPNTNYLSLKNESSFNGDDKFEITR
                     NWMENKNEIVVKGKFPGSSRYVKNSVNVLEPEKYFLKLFSEVLDSNSIKLSGIIGLKN
                     TPLYAKQFCTFKRKFSDVLDNLNKTSDNLSAEMTLYALANKYSGKPAIADSGVQFINQ
                     MIDSLGLDHEDYRIVDGSGVSHYNVVSTELLVNIL"
BASE COUNT          657 a          241 c          335 g          605 t
ORIGIN      
        1 aagttgctga tattcataaa atttctttag aagatgttgg cagaataact taattaaata
       61 cttttagatt ttttggaatt ggtaaagcac caaaaactaa ttttacttat ttgattggga
      121 attctttata catcaacgtt acaaacaggt gtaatgcgga ttgcactttt tgcagaagaa
      181 aagaagatcc atttttaaat gggtataatc ttggaatgaa aaaatctgaa gaaccgcctg
      241 ctgaagttta tattagtgaa atcggtgatc ctaaaaaata tgatgaaatt gttttttgtg
      301 gatacggtga accaacaatt cgctgggatg ttgttaaaga agttggaagg tatgttaaag
      361 ctaacggcgg cagtaccaga attaatacaa atggtcacgg aaatattata aacaaaaaag
      421 atattactcc cgaaatgaaa gatattatcg atgttgtttc gataagcttc aatacatttg
      481 atccaaaaca atatgcggaa ttaatgagag tcagcgaaga tcattttgaa gaaatgaaaa
      541 aatttgcttc tttagcaaaa ccctatgtaa agaaagttgt gatgagtgtg gtttcattag
      601 atgaagttga aattgaaaaa tcacgcaaag ttgtcgagga agaaatcggt gttgaattca
      661 gagttagaga gtatttctaa attaaattgt aatgtcatct ccgcaaaggc gggaatctaa
      721 ggtttagata tgaaaagaat attaatcctg tcagttatct tttttatttc tgcttatccg
      781 caatccttaa aaacaaaact tgataatgtt ttaaaagata aattctttga aagttgttta
      841 gtctcaattc aggttgaaga tttgactaca aacaaaactc tatataaaga caatgaaaaa
      901 atgctgcttc gacctgcatc aaatatgaag gtaataactt cttctgcagg attattattt
      961 cttggtcccg attatgagtt tacaactaat ctctatcatg atggatatgt ttctaatgat
     1021 actctttacg gagacattta tgtggttgga ggctgtgatc ctgattttat tacacaggat
     1081 ttatacactt ttattgatac aataaaatta ttaaacattt ctgttattaa cggaaatatt
     1141 tatggtgatg tttcatttaa agattctctg tactggggta aggggtggat gtgggatgat
     1201 aatccttcgt cggatgcacc atatttaagt gctttaaata taaatgataa ttgtgttgaa
     1261 gtatttgtac aaaaatcaga atacctggat aaacctgaag tcagcctaat tccaaatact
     1321 aattatcttt ccctaaaaaa tgaatctagt tttaatggtg atgataaatt tgagattaca
     1381 cgtaattgga tggaaaataa aaatgagata gttgtaaaag gtaaatttcc aggctcaagc
     1441 agatatgtaa aaaattctgt aaatgttctt gaacccgaaa aatattttct taaactattt
     1501 tctgaagtgc ttgattcaaa ttcaataaag cttagcggaa taattggtct gaaaaatact
     1561 ccattgtatg ccaaacaatt ttgcaccttt aaaagaaaat tcagtgatgt tttagataac
     1621 ctgaacaaaa caagtgataa cctcagtgca gaaatgactt tgtatgcact tgcaaataaa
     1681 tactccggaa aacctgcaat cgcagatagc ggagttcagt ttataaatca aatgattgat
     1741 tcacttggat tagatcacga agattataga attgttgatg ggtccggagt ttcacactat
     1801 aatgttgtta gcacagaact gcttgtaaat attttaaa
//
LOCUS       DASWBY010000486         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_246366, whole genome shotgun sequence.
ACCESSION   DASWBY010000486 DASWBY010000000
VERSION     DASWBY010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246366"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /gene="eno"
                     /locus_tag="VF870_08680"
     CDS             complement(<1..237)
                     /gene="eno"
                     /locus_tag="VF870_08680"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9252303.1"
                     /translation="MTTIVDVFGREILDSRGNPTLEVEVYLEGGVMGRAAVPSGASTG
                     EHEAVELRDGDKSRYNGKGVLKAVENVNEKIADEI"
     gene            611..2533
                     /locus_tag="VF870_08685"
     CDS             611..2533
                     /locus_tag="VF870_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9252304.1"
                     /translation="MHYDTLILLIQFAGLLLFARLLGELAQRFGQPAVVGEILAGILL
                     GPTLLGNVHIISEYMVPTESGKNYLTIISMIGAMFLMFIAGMETDIKLIKIHSKKAIT
                     IASTALLVSFISVFLFSLTIPDNLLTNPDSKFLFAAFLSITISVSAISVIAKVLIDLN
                     LIRRDFGQLFIAIGMIDESIVWIIISIIIGLVGGFAATAGGIFLSFIKVILFLVLGYG
                     VGKFLIKYIITFFQNKIKLRDKFLTLVVVIIFIYGAIAQSLGLEAVLGAFLAGIIFGQ
                     IPYLSNETIDKIESITFAIFAPIFFASAGLQVNILEFTNPSIITITLLLMLIAFTSKT
                     IGGFVGAKFFAKSDTNTSLLFGIGLNTRGTIQIILATIGLSIGILSKDIFSIIILISV
                     VASVISPIFIKIIIKKIEPNKEEIQRINQEEIYAKSILSKINRILLPVRVRTDLNAEA
                     AKSIEANIIEAIASKKQISVTLLSITAEQDKNKATEFLEDIAKSFTKSEVTKKVIVAN
                     NALEMILEESKKDYDLLVLGATEKQSNSDMVFNTFIDNLIRFSPCPSLIVQDHAVFED
                     NKFKKILVPTDGSKASKRAAEVAFALTLNKKDEVHLLRVVEERISLDELETKNHILDR
                     QFNYAKEIVNELKAIG"
BASE COUNT          874 a          405 c          401 g          893 t
ORIGIN      
        1 aatctcgtca gctatttttt catttacatt ttcaaccgct ttaagaactc cttttccgtt
       61 gtagcgggat ttgtcgccgt ctctcaattc aactgcttcg tgttcaccgg tagaagcacc
      121 gcttggaact gcagctctac ccatgactcc accttcaaga taaacttcaa cttctagagt
      181 tggatttcct cttgagtcta atatttctct tccaaataca tcaactattg ttgtcatttt
      241 aataactcct gatttttcta aatgtgattc taaattttgt ttaatttgct tatcaaaatt
      301 agctaaatgt gaaatggaat tgaaatgatt tgagactaaa tgatggtaaa ttggttctaa
      361 atagtggcaa aaattattta cacgaattat tactaattct taaaaatttg attttattac
      421 aatttataat ttgagggaac aattttagtc aaacgtttta aaagcttatc aataaattta
      481 gttccatcac aaaaagttca gagaattata ttttggttaa taattcaatt tcaactttaa
      541 actgctgtgt cttttaattc taaaacaaac tatcttgcca acaaattaat aacaaattat
      601 tttttaacgt ttgcactacg atacactaat attattaatt cagtttgccg gattacttct
      661 gttcgccaga ttattaggcg aacttgcaca gcgattcggg cagcctgcag ttgttggaga
      721 aattttagcc ggaattttac ttgggccaac cttacttggt aatgtacata taatatcaga
      781 atatatggtc ccaaccgaat ccggaaagaa ctatcttaca attatcagta tgattggggc
      841 aatgttcttg atgtttattg caggaatgga aacagatatt aagctaataa agattcattc
      901 taaaaaagct attacaattg catctactgc attacttgtt tcttttataa gcgtattcct
      961 tttcagttta acaattccgg ataatctttt aacaaatcct gattcaaaat ttttattcgc
     1021 ggctttctta tccataacaa tttctgtatc agcaatttct gtaatcgcta aagtgcttat
     1081 agatttaaat cttataagaa gagactttgg acaattgttt attgctatcg gaatgattga
     1141 tgagtcaata gtatggatca taatttcaat tattattggt ttggtcggtg gatttgcagc
     1201 aactgcaggt ggaatatttt tatcatttat taaagtgatt ttatttcttg ttcttggtta
     1261 tggagtagga aaatttctaa taaagtatat catcacattt tttcagaaca aaattaaact
     1321 gcgtgataag ttcctaactc ttgttgtggt gattatattt atctatggtg caattgctca
     1381 atcgcttgga ttagaagctg tacttggagc ctttcttgcc ggaataatat ttggacaaat
     1441 tccataccta tcaaatgaaa caattgataa aattgaaagc ataacttttg caatcttcgc
     1501 tcctatattt tttgcatcgg caggattgca ggtaaatatt ttagagttta caaatccaag
     1561 tattataaca attactcttt tattaatgct tattgcattc acttccaaaa caatcggagg
     1621 atttgttggc gccaaattct ttgcaaaaag cgatactaac acatcgcttc tatttggaat
     1681 cggattaaac acccggggca caattcaaat tattcttgca actattggtt tatcgattgg
     1741 aattttatcc aaagacattt tctctataat aattttaatt tctgtagtgg cttcagtcat
     1801 ttctccgatc tttataaaaa ttattatcaa aaagattgaa ccaaataaag aagaaattca
     1861 aaggatcaat caggaagaaa tttatgctaa aagtatttta tcaaagatca acagaatatt
     1921 actaccggta agagttagaa ctgatcttaa tgcagaagca gcaaaatcaa ttgaggcaaa
     1981 tattattgaa gcaattgcat ctaaaaagca gataagtgtt acactacttt ctataaccgc
     2041 agaacaggac aaaaataagg ctacggaatt tttagaagac attgcaaagt catttacaaa
     2101 atccgaagta actaaaaaag taattgtagc aaataatgca ttagaaatga ttcttgaaga
     2161 atcaaaaaag gattatgatt tattagtact tggagcaacg gaaaaacaaa gtaattccga
     2221 tatggttttt aatactttta tagacaacct tataagattt tcaccatgtc cttctttaat
     2281 tgttcaagat catgctgttt ttgaagacaa taaattcaaa aaaattcttg ttcctacaga
     2341 tggctccaaa gcctctaaaa gagcggctga agttgcattt gcattaacgt taaataaaaa
     2401 agacgaagta catcttctaa gagttgtaga agaaagaata agtcttgatg aactggagac
     2461 taaaaatcat attttagata gacagtttaa ttatgctaaa gaaatcgtta acgaactaaa
     2521 agctatcggc taatcacttt ccgtaaatac gcataccaga gttgagattg gat
//
LOCUS       DASWBY010000487         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_433298, whole genome shotgun sequence.
ACCESSION   DASWBY010000487 DASWBY010000000
VERSION     DASWBY010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433298"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VF870_08690"
     CDS             <1..469
                     /locus_tag="VF870_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9252305.1"
                     /translation="WEEVHKINSTGVMLGCKYGIKLMKEKGGSIVNISSRSGIVGIPG
                     AVAYAASKAAVRNHTKSVALYCAEKKYKIRCNSIHPAAIMTPMWDAMLGSGEQRKQFI
                     KFIEAGIPLGHFGDPLDVAYGALYLASDESKYVTGIELNIDGGILAGSEAKPQ"
     gene            complement(503..1294)
                     /locus_tag="VF870_08695"
     CDS             complement(503..1294)
                     /locus_tag="VF870_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9252306.1"
                     /translation="MKKYQWDADQYFRFSSAQQKWARESIDKAKIKSNEHVLDVGCGD
                     GKVTVEIAELVTEGKVLGVDNSDSMINLAEEKFPTSKYPNLSFKVLDAKELNFNEEFD
                     VILSNAALHWVDDHLKVLKGMYNSLKKGGRILLQMGAKGNVPEAFFSVEKVISKPEWS
                     GYFLGMGFPYYFFNEEEYTSLILQSGFSDYKIYVEEKDMQHKGKEALKGWIRTTWLPY
                     TQKVPEEQREKFIEEAFNVFAENFPADKNGIFHASAKRLIVEATK"
BASE COUNT          488 a          275 c          257 g          486 t
ORIGIN      
        1 ctgggaagaa gttcataaaa tcaattcaac cggagtaatg ctaggatgca agtatggaat
       61 taaacttatg aaagaaaaag gcggcagcat tgttaacata tcctctagaa gtggaattgt
      121 tgggataccg ggcgctgttg catacgctgc aagcaaagct gcagttagaa atcatacaaa
      181 aagtgttgct ctttactgtg cagagaaaaa atataaaatt aggtgtaaca gtatccatcc
      241 cgcagcaatt atgactccaa tgtgggacgc aatgcttggg tcaggggagc agagaaagca
      301 atttattaag tttattgaag caggaattcc tttaggacat tttggcgatc cgttggatgt
      361 tgcgtatggc gcattatatc tggcaagtga tgaatcaaag tatgttaccg gaattgagct
      421 gaatattgat ggtggaatac tggcaggcag tgaagcaaaa ccacaataga ttttattgtg
      481 aatcttaact aatgctattt agtcattttg ttgcttcaac aattagtcgt tttgcacttg
      541 cgtgaaatat tccattttta tcagcaggaa aattttccgc aaaaacatta aaagcttctt
      601 caataaattt ttctctttgt tcttcaggaa ctttttgtgt gtaaggtaac caggtagtgc
      661 gtatccatcc tttaagtgcc tcttttcctt tatgctgcat atctttttct tcaacgtaaa
      721 ttttgtaatc actaaatcct gattgtaaaa taagactggt atattcctct tcattaaaaa
      781 agtaataggg aaatcccatc cctaaaaagt aacctgacca ttccggtttt gaaataactt
      841 tctccacaga aaaaaaagct tcgggaacat ttcctttagc tcccatttgt aaaagaattc
      901 gtccgccttt tttcaaacta ttatacattc ctttaagcac tttaagatga tcatccaccc
      961 agtgaagagc ggcattggaa agtatgacat caaattcctc attaaaattc aattcctttg
     1021 catcaagcac tttaaaagaa agattagggt attttgatgt gggaaatttc tcttcagcca
     1081 ggtttatcat tgaatcagaa ttatccacgc caagaacttt tccttcagta actaattctg
     1141 ctatttcaac cgtaactttc ccatcaccgc agccgacatc aagcacatgt tcatttgatt
     1201 ttattttagc tttatctatt gattctcttg cccatttttg ttgagcggat gaaaaccgaa
     1261 aatattgatc agcatcccat tgatattttt tcattttttg cctcctaaac aatcaacatt
     1321 cagatgaaaa ataatataat tttttgcctc tgcaaataac taaatcgatc aaaattttgc
     1381 tgtatcttta agaaatgata aagaggcaac aatagttaaa acatttgcaa caattgttaa
     1441 aatagcccca aaagggtaaa tagctctgca acaattctta aaaacattgc atcaattctg
     1501 aaatcc
//
LOCUS       DASWBY010000488         3494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_356037, whole genome shotgun sequence.
ACCESSION   DASWBY010000488 DASWBY010000000
VERSION     DASWBY010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356037"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VF870_08700"
     CDS             complement(<1..547)
                     /locus_tag="VF870_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9252307.1"
                     /translation="MKALVIDDERLARTELIRLLEPFKEIEVVGEAVNADDAHNKISE
                     LNPDVIFLDIQMPGKTGFELLAELDSVPKVIFTTAYDEYALKAFEYNALDYLLKPIEP
                     ARLEESIKKLIEGKRKERTHDLSHETLTAEDQVFVKDGDKCWFVKLEKVRLMESEGNY
                     VRLYFDENKPLILRTLNYLDER"
     gene            complement(544..1668)
                     /locus_tag="VF870_08705"
     CDS             complement(544..1668)
                     /locus_tag="VF870_08705"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9252308.1"
                     /translation="MNTAILNTKLNFSDDKPVTKTDIIDTLKRKNLYWVSQASGWSLF
                     VIINLLIISSFETIPLNRIFLWILLGFYGLIFSHLYRLYIKKNNWTSLPLKKIIPRIL
                     IASLAGGLIIYIPVFISGHLLGVERDAQHMTAAIISSILNITSVITVWSLIYFAVHYF
                     ENSKKAEIETLIFEAAVKDFELKTLKAQLNPHFMFNAMNSIRALIEEDPQNAKDAITK
                     LSNLMRYTLKIERTETVSLAEELRTIQDYLDLEKIRFEERLNYKIHSTPDADRVEIPP
                     MMVQTLVENGIKHGISKITSGGQVDIDAKVINSNLIIEIRNTGRFDEEALKNSRGFGV
                     SNTKHRLSLLYGENASLALTNENKNTVLTKLKIPSGGSKQ"
     gene            complement(1923..3278)
                     /gene="rseP"
                     /locus_tag="VF870_08710"
     CDS             complement(1923..3278)
                     /gene="rseP"
                     /locus_tag="VF870_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX9252309.1"
                     /translation="MDYIIYFAITIGILVFIHEFGHFAAAKLSGMRADVFAIGFGKRL
                     FGYNKKSGFSFGELPKDFDGEGHTDYRLSLLPLGGYVKIAGMVDESGDVSFADKEPQP
                     YEFRAKPVWAKVFVITAGVFMNLMLAWIIFWGANFFQGKPITPTTTIAYVEEGSPASI
                     AGFETGDKILSVNNKSVNTWEDLRAEIFINTLGENINVKVLRDGKEQNLLVERKLIPD
                     DETQSMFLIPEGVKPAIGEVVKDSPAEKAGIKAGDILLSLNGVPLYTAKQTTEIISSN
                     PEKELQLVVQRDKKDVNITVTPGKDAKIGVAIGPVFMGESKMRTYGAFESVYYGWKDI
                     EKMTALTFSMIGKVFTGDVEFKKAFGGPIKIAQIAAKSADSGMSSFLYFLALLSLSLA
                     ILNIMPFPVLDGGHLVMILIEAAIKREIPIKIKMAIQNTGLVLLLLLMAFIIYNDIIS
                     L"
BASE COUNT         1092 a          657 c          541 g         1204 t
ORIGIN      
        1 atctttcatc cagataattt aaggttctta agataagagg tttgttttca tcaaaataca
       61 atcgcacata atttccttcg gattccatta aacggacctt ctctaattta acaaaccagc
      121 atttatcacc atctttaaca aacacctgat cttctgcggt taatgtttca tgagataaat
      181 cgtgagtacg ttctttacgt ttgccttcaa ttagtttttt aattgattct tctaatcgtg
      241 ctggttcaat tggttttaaa agataatcta gtgcattgta ctcaaatgct tttaaagcgt
      301 attcatcata cgcggttgta aaaattactt ttggaacgct gtctaattct gcaagcaatt
      361 caaatcctgt tttgccaggc atttgaatat ccagaaaaat tacatccgga ttcaactccg
      421 atattttatt atgtgcatca tcggcgttga ccgcttctcc aacaacttca atctctttaa
      481 atggttctaa taatctgatt aactcagtgc gcgcgagtct ttcatcgtct ataactaatg
      541 ctttcattgc tttgaacctc ctgatggtat tttaagcttt gtaagaactg tgtttttatt
      601 ttcatttgtt agggctaaag aagcattttc tccatatagc aaagataatc gatgctttgt
      661 attacttaca ccaaatccac gggaattttt taatgcttct tcatcaaacc tgcccgtgtt
      721 tctaatttct attattagat tagaattaat aacttttgca tctatatcga cctgtccgcc
      781 tgaagttatt tttgagatac catgtttaat tccgttctca actaaggttt gcaccatcat
      841 tggcggaatc tcaacccgat ctgcatcagg agttgagtga attttataat tcaatctctc
      901 ctcaaatcta attttttcaa gatcaagata atcttgtatt gttctcaatt cctctgcgag
      961 tgaaacagtt tctgttcttt caatctttaa agtataacgc attaaattag aaagttttgt
     1021 gattgcatct ttcgcattct gcggatcctc ttcaattaat gcacggatgc tgttcattgc
     1081 attaaacata aaatgcggat taagctgggc ttttaaagtt tttaattcaa aatcttttac
     1141 ggcggcttca aaaattaaag tttcaatttc tgccttcttt gagttttcaa agtagtggac
     1201 agcaaaataa atcaaagacc aaacagttat tacgctggta atatttaaaa tactcgaaat
     1261 tatagctgca gtcatatgct gggcatctct ttcaacaccg agtaaatgac cagaaataaa
     1321 aaccggaata tatattatca atccacccgc cagacttgct attaaaattc tagggattat
     1381 tttttttaat ggaagactgg tccagttatt ttttttgata tagagtcgat acagatgaga
     1441 aaaaattaat ccataaaatc ctaaaagtat ccagagaaat attcgattta gagggattgt
     1501 ttcgaaagaa gaaatgataa gtaaatttat aattacaaac agactccagc cagaagcctg
     1561 agaaacccag tataagtttt tccttttcaa tgtatctata atatcagttt tagttacagg
     1621 tttatcgtca ctaaaattta attttgtatt aagtattgca gtgttcatat caaatataat
     1681 cttaacctaa ataaaagaga accaactttt aagtttgcag gggcaactta ttggaagtag
     1741 tagttataat tgagatgttt tttatgtgtg gcaaagaaat gtgataggtg gaaaattact
     1801 tgatgtcatt ctgatcgaag tccaaggatg accattccat aaatggaata aaactgtcac
     1861 acttcgtccc tcgactacgc tcgggatgac acgctcagtg tgacaatttt attaaaaata
     1921 gattataaag aaattatgtc attataaatt ataaatgcca ttagcaataa aagcagtaca
     1981 agtcctgtat tctgaattgc cattttaatt ttaattggta tctctcgttt gatcgcggct
     2041 tcaattaata tcattaccaa atgtccgcca tctaatacag ggaaaggcat aatgtttagt
     2101 atcgccaagc ttaaacttaa taatgccagg aagtacaaaa agctggacat accggaatca
     2161 gctgatttgg cagcaatctg tgcgatttta attggaccac caaaagcttt tttgaattca
     2221 acatcacccg taaaaacttt tccgatcatt gaaaaagtta atgcagtcat tttttcaata
     2281 tctttccagc cataataaac agattcaaaa gcaccgtagg ttcgcatctt ggattcaccc
     2341 ataaaaacgg gaccaatcgc tacaccgatt ttagcatctt taccgggagt aacagtgatg
     2401 ttgacatctt ttttatctct ttgtacaaca agttgtaatt ctttttctgg atttgacgaa
     2461 ataatttccg ttgtttgttt tgcagtgtaa agtggaactc cgttaagact taaaagaata
     2521 tctcctgctt ttattcctgc tttttctgca ggcgaatctt ttacaacttc tccaattgcc
     2581 ggcttaactc cttccggaat taaaaacatc gattgtgttt catcatcggg aattaatttt
     2641 ctttcaacta aaagattttg ttctttaccg tctcgtaaaa cttttacatt aatgttttcg
     2701 ccaagggtgt tgataaaaat ttctgcacgt aaatcttccc aggtattaac acttttatta
     2761 tttacactta aaattttatc gccagtctca aatccagcta ttgaagcagg actgccttct
     2821 tcaacataag caatagttgt agttggagtg attggttttc cctgaaagaa atttgcaccc
     2881 cagaatatta tccaggcaag cataagattc ataaataccc cggcggtaat tacaaatact
     2941 tttgcccata ctggttttgc acgaaattca tatggctgcg gttctttatc tgcgaagcta
     3001 acatcaccac tttcatcaac catgccggct attttaacat aaccgccaag aggtaataaa
     3061 cttaatctgt aatcggtatg accttcgcca tcaaaatctt ttggaagttc accaaacgaa
     3121 aatccagatt ttttattata tccgaataat ctttttccaa agcctatagc aaaaacatct
     3181 gcacgcattc cgcttaactt agcggctgca aagtgcccaa actcgtggat gaaaaccaaa
     3241 ataccgattg taatagcgaa ataaattata taatccatgt aaacaggaac tccttattct
     3301 aactaaactt aatattcatt ttgttactta tactttttaa ttaaccgatc ctggattctt
     3361 gatactggat actagtttct cgatcctgga tgctgctaaa ttatttatca cttaaatgat
     3421 ccgcttgaac tgattgtaaa aagcgattaa tctttttccc aataattcta aaactgctgt
     3481 cacactgagc gtag
//
LOCUS       DASWBY010000489         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_94144, whole genome shotgun sequence.
ACCESSION   DASWBY010000489 DASWBY010000000
VERSION     DASWBY010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94144"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VF870_08715"
     CDS             <1..246
                     /locus_tag="VF870_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252310.1"
                     /translation="MTFEEFVKPKYGDKVAFNELESYHTLKKVEYYLIVLNEQYSPEK
                     TEEDYRLFLQSITNDSNLIDTILKAWTKVLASLERED"
     gene            247..669
                     /locus_tag="VF870_08720"
     CDS             247..669
                     /locus_tag="VF870_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252311.1"
                     /translation="MDIQQVVAADRVKFRKPISIVKEYLDFAMQMEPQSRVRVVADYP
                     TGWHAEVVASNKLWLIACIFGFINLLNVSDCRGFRPNGSFVILKTEDAIEYEAHFLGI
                     DFDRDSLFSPLTCDSFFLRDELPCLPYCKKRFKEEDQE"
     gene            692..1351
                     /locus_tag="VF870_08725"
     CDS             692..1351
                     /locus_tag="VF870_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252312.1"
                     /translation="MTEEKVEIEQFPSNSGGKLKEEPEPEEESKKIVKQVATGKRIVK
                     KKTFWDTMLNGTLKVVGSYLLFEILIPAAKNMVSDMINNGSDMILFGETKSRRRDRER
                     GRGSSYVSYTSYYDRDRDRGDRPYDRERRDRVYGRSRFDSDEILLPSREEAEEVIQSM
                     FDIYDQYNAVTVSEFLELVGLPDEYTDRNYGWTNLRDIQARRVRDGYIIDLPVPKALP
                     R"
     gene            1362..1529
                     /locus_tag="VF870_08730"
     CDS             1362..1529
                     /locus_tag="VF870_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252313.1"
                     /translation="MIEKIPIKFHVEGNKLIFDEMWIILTGDKVVIEHSMDENGVIHY
                     TKVWIERRITS"
     gene            1540..1746
                     /locus_tag="VF870_08735"
     CDS             1540..1746
                     /locus_tag="VF870_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252314.1"
                     /translation="MEKLILYKIKGFNTNLNNRFKALVVARSYDEAFNLGNELAGPGG
                     WISSIKETGKEIFLKGEKQNDQSE"
BASE COUNT          696 a          240 c          405 g          583 t
ORIGIN      
        1 atgacttttg aagagtttgt taaaccaaaa tatggtgata aagttgcttt taatgaactt
       61 gaatcctatc acactctaaa aaaggttgaa tattatttaa ttgtattaaa tgagcaatat
      121 tcgccagaaa aaaccgagga agattatcga ctatttcttc agagtattac taatgatagc
      181 aatttaattg atacaatttt aaaagcttgg acaaaagttc ttgcaagttt agaaagggaa
      241 gattaaatgg atatacagca ggtagtcgcg gcagatcgag taaaattcag aaaacctatt
      301 tcaatagtaa aagaatatct tgattttgct atgcagatgg aaccccaatc aagagtcaga
      361 gttgttgcag attatccaac gggatggcat gctgaagtag tagccagtaa taaactttgg
      421 ttaattgcct gtatatttgg gtttattaat cttttaaatg tatcagattg tcgaggtttt
      481 agacccaatg gatcatttgt tatattgaaa acggaagatg ctatagaata tgaggcccat
      541 tttctaggaa tagattttga tagggattct ctttttagtc cattaacttg tgattccttc
      601 tttcttcgag atgaattacc gtgtttacca tattgtaaaa aaagatttaa agaggaagat
      661 caagaataat tcttgaaagg agttttataa aatgacagaa gaaaaagtag aaatagaaca
      721 atttcctagt aattcgggtg gcaagttaaa agaagaacca gaacctgaag aagaatcaaa
      781 aaagattgtt aagcaagttg ccacagggaa aaggattgta aaaaagaaga ctttttggga
      841 tactatgctt aatggtactc ttaaagttgt aggaagttat cttctttttg aaatcttaat
      901 ccctgctgct aagaatatgg tatctgatat gattaataat ggtagtgata tgatcttatt
      961 tggtgaaacc aaaagtcgaa gacgggatag agagcgagga agaggttctt cttatgtaag
     1021 ttataccagt tactatgatc gcgatagaga tcggggagac agaccttacg atcgagaaag
     1081 acgagataga gtttatggac gatctcgttt tgattcggat gaaatccttc taccaagtag
     1141 agaagaggct gaagaagtta ttcagagtat gtttgatatt tatgatcaat ataatgctgt
     1201 aacagtatcg gaatttcttg aactggttgg acttcctgac gaatatactg atcgaaatta
     1261 tggttggact aatcttcgtg atattcaggc tcgaagagtc agagatggtt atattataga
     1321 tcttcccgtt ccaaaagcct taccaagata ggggaaaata aatgatagaa aaaattccta
     1381 ttaaatttca tgttgaaggt aataaactta tatttgatga aatgtggatt atactaactg
     1441 gtgataaggt tgtaattgag cattctatgg atgaaaatgg tgtaatccat tacacaaagg
     1501 tttggattga acgaagaata acttcttaaa aggagaaaaa tggaaaaact aattttgtat
     1561 aaaataaaag gttttaatac aaatttgaat aatagattta aagccttggt agttgctaga
     1621 tcatacgatg aggcatttaa tctaggtaat gaacttgctg gtcccggtgg atggataagt
     1681 tcaattaaag aaacaggtaa agaaattttt ttaaaaggag aaaaacaaaa tgatcagtct
     1741 gagtaatata aaaacgctgg caacaaactc cttaggacga acgggcttaa tattgagtaa
     1801 aaggagtccg gaaatcatgt taggagtggg tgttgccggg attgttgtag gcactgtgct
     1861 agcttgcaaa gccacattaa aagttgaaaa aatcactaag gaagcaaaaa caactcttaa
     1921 tact
//
LOCUS       DASWBY010000490         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_433367, whole genome shotgun sequence.
ACCESSION   DASWBY010000490 DASWBY010000000
VERSION     DASWBY010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433367"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VF870_08740"
     CDS             complement(<1..214)
                     /locus_tag="VF870_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9252315.1"
                     /translation="MTKDIKQNNFSTLKVESCDNGLVIVTLNRPEAANAVNTEMGIEL
                     LDLWTDFVRNHEGLRCVILTGAGEDAF"
     gene            complement(302..1522)
                     /locus_tag="VF870_08745"
     CDS             complement(302..1522)
                     /locus_tag="VF870_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252316.1"
                     /translation="MANQVKRTAPDMDKIIAEAKKAGKLKTVPLDWNEVYYTNCPLVS
                     ASNVDQELGWTKEEYKKIGVHYLYLRARKENDWYPHYIHNMDNIIRFGGLFPPVHVHA
                     DIRRTVLLGVTHVEREGGVMLVRSRDDIYRMAELKGKKIGLSRSQNKIKTDWWGIQEE
                     QGIELMLRLNGMTRADVEIVEFPYPDDWYDVPEMMGPEMENPSELWLRRDHKHDLAFR
                     PLETALEKGVIDAMYMQSKPLQQLSEATGKFKAIEDLSRYPDWTLQVANIPAAITCTV
                     EMAEKHPELAVTFLKGMIKVGRWANEHKHAAAAILDKQTFYLDVEHTYRGIEHVDMVP
                     NLSPQNLRSVEIGKDFMLSHGYIKNDFDVNKWAAPQFLEQAARELLELEWKKRTVSKL
                     PQAADPLASGGRMG"
     gene            complement(1553..1720)
                     /locus_tag="VF870_08750"
     CDS             complement(1553..1720)
                     /locus_tag="VF870_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252317.1"
                     /translation="MDKKNKTKEIDIHCACAIGDLSELAKINPANKTETKIQKKILKK
                     GDLKKEDSNHK"
     gene            complement(1803..2069)
                     /locus_tag="VF870_08755"
     CDS             complement(1803..2069)
                     /locus_tag="VF870_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252318.1"
                     /translation="METVTISAMISDQKKAEFFQTTESLKSSITKYCNDFKIDVDADN
                     LLEIKIEFEDTRQLENYYQRTEFNILKGSLKSLCDNIEIKISDV"
BASE COUNT          636 a          497 c          420 g          858 t
ORIGIN      
        1 aaaatgcatc ttcacctgcc ccggtcagga taacacagcg aagtccttca tggttacgca
       61 caaagtctgt ccacagatcg agtagttcga tacccatttc agtgttgaca gcgttagccg
      121 cttcgggccg gtttagtgtt acaataacta atccgttgtc acaactttca accttgaggg
      181 tggaaaaatt attttgctta atatctttgg tcatatttac atttaattta tttagttaat
      241 cgtaatgata tttattatgc taattctcaa ttccaaataa tctcaattag ctttctttca
      301 attaacccat tcttccacca gatgccaacg gatctgctgc ctgaggaagt tttgaaacag
      361 ttcttttctt ccattccaat tccagcaatt ctctcgctgc ctgctcaagg aactgaggtg
      421 cggcccactt gtttacatcg aagtcgtttt tgatgtaacc atggctcaac atgaaatctt
      481 tacctatctc aacagatctt aggttttgag gtgacaggtt tggaaccata tcgacatgct
      541 cgatgcctct atatgtatgc tcaacatcga gatagaatgt ctgtttgtct agtatggctg
      601 ctgctgcgtg cttatgttcg ttagcccagc gtccaacctt tatcatacct ttcaaaaaag
      661 ttactgcaag ttccggatgc ttttctgcca tctcaactgt gcaggtaata gctgcaggaa
      721 tgttagctac ctgcaatgtc cagtccggat accttgacag atcttcaata gctttgaatt
      781 ttccagtagc ttccgaaagc tgctgaagtg gtttggactg catatacatt gcatctataa
      841 cacctttttc caaagctgtt tccaatggac ggaatgcaag gtcatgttta tgatcacgac
      901 gaagccataa ttccgatgga ttttccatct caggacccat catttcaggc acgtcatacc
      961 agtcgtcagg ataagggaat tcaacgattt caacatcagc acgagtcata ccattaaggc
     1021 gtagcatcag ctcgataccc tgttcttcct ggatgcccca ccagtcggtt ttaattttgt
     1081 tctggcttct tgatagtcct atttttttac ctttaagttc agccatgcgg tagatatcgt
     1141 ctcttgaacg taccaacatt acaccacctt cacgctcaac atgtgttact ccaagaagca
     1201 cagttctacg gatatctgcg tgaacatgta ctggcggaaa gagaccgcca aacctgatta
     1261 tattatccat attgtggata taatggggat accagtcatt ttctttacgg gcacgaagat
     1321 aaaggtaatg aacacctatc ttcttgtatt cttccttagt ccagcctaat tcctgatcca
     1381 cattactggc cgataccagc ggacaattag tgtaatatac ttcattccag tctaacggaa
     1441 cggttttgag cttgccggct ttttttgctt ctgcaataat tttgtccata tccggtgctg
     1501 ttctttttac ttggtttgcc ataactgaat tccttctttt gtttaatgat tattatttat
     1561 gatttgaatc ttcttttttt aaatcgccct ttttcagaat tttcttctgg atctttgtct
     1621 ctgttttatt tgcgggattt atctttgcta attccgataa atccccaatt gcacaggcac
     1681 aatggatatc tatctctttt gttttgtttt ttttgtccat aatagtactc ctcattattg
     1741 attgtaaact tttaaaaaga tattagctta actgaaccga acgattcgaa ttaactagct
     1801 ggctacacat cactaatttt aatttctatg ttatcacata aactttttaa agaaccttta
     1861 aggatgttaa actcagtcct ctgataataa ttttccagct gacgtgtgtc ttcaaattcg
     1921 attttaattt ccaggagatt gtctgcgtca acatctatct taaaatcatt gcagtatttc
     1981 gtaattgatg atttaagaga ctctgtggtt tgaaaaaatt cagctttttt ctgatcactt
     2041 atcatggcac taattgtaac agtttccata attttattac ttttaataat gatactatta
     2101 cttaaatagt tgtttacatt tatcaggtaa tagtcaactg atcagccagg gttagatatt
     2161 tagaaattag atggtattga aggttttaag aatattcagg acttgaatac tcgcttaatt
     2221 tagcgactgt cgctaaatat agcgaaatat tatcacgcct atttacgttt aattctgagt
     2281 ttaagcatac gtgagcgaag tgtttgcgga ttgattttaa gtaactctga agcactgttg
     2341 gggccgctga ttttccagcc tgttttttca agtgtctcta ctatatgatt tatttcattc
     2401 tcttcaagtg t
//
LOCUS       DASWBY010000491         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_246450, whole genome shotgun sequence.
ACCESSION   DASWBY010000491 DASWBY010000000
VERSION     DASWBY010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246450"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2127
                     /locus_tag="VF870_08760"
     CDS             <1..2127
                     /locus_tag="VF870_08760"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9252319.1"
                     /translation="GLDKLDYDKKLFTNYSHNAANPNSLINNSVLVITGDKFGVLWLG
                     TFSSGMNSFDPKTNSFKQFINNPSDPNSLSDNRVYSIFEDRQGTIWIGTYGGGLNRFD
                     RSTGKFKVYKHIENNSSSISSNSVWTIAEDNEGKLWLGTFGGGVNIFDKNKQTFTSFR
                     NDPNDPTTLGDDNIIRVFKDSKNNMWLGTTKGLCRFYSDTKKFKTYREKDGLANDFVY
                     GILEDDKGNLWLSTNNGLSKFDPKKESFKNYYVIDGLQGNEFNQNAFAKDFKTGRLLF
                     GGPNGLNIFNPEDLKDNDYLPPIALTNYLRYNTDDEEGKPIIEKGISERDSILLTFKD
                     NIVTLEFSALSYFNNYHNQYKYKLEGFNENWIQLGNNHTITFTNLSPGEYYLKIIGSN
                     NDGLWNENGATLFIEVLPPWWRTNIAYTIYGILFVSILYGLRRFEMNQHKQKAHIRET
                     ELKMKATEAEKRALEIENERKTKELEEARQLQLSMLPKKLPVLPNLEIAAFMRTATEV
                     GGDYYDFRVLENGSLNVAFGDATGHGLQAGTMVTLMKGFFTSDSSKLGMPEFMNHCSK
                     MIKEIELGRILMSFSYLKFEDKKIYITSAGMPPIFYYDNNANTVEEIIIQGMPLGAMK
                     KFPYKVIDKEIKSGDTILLLTDGLPEQMNNNEEMFDYSRVKSSFHSVINNSPEEIINS
                     LVNEGDKWMNGRVQDDDISFVVIRIK"
     gene            2237..2653
                     /locus_tag="VF870_08765"
     CDS             2237..2653
                     /locus_tag="VF870_08765"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9252320.1"
                     /translation="MKKNVKLEIKLPNIPDVELVAIESLQIMGRHCGISEDKIGEAKI
                     IVTEAIINAIEHSGENSPYVLIEFDMDTEKLIILVTDYGTGFEPDSVEEPNILDKIHS
                     PHKRGWGLKLMKSMSDDLIIESGPNGTRITIIKNLI"
     gene            2667..3002
                     /locus_tag="VF870_08770"
     CDS             2667..3002
                     /locus_tag="VF870_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9252321.1"
                     /translation="MSTEFNLMSELHDKTLVIKTEGYINNIGGEKIVQEFGKHNGVNR
                     LILNLENSKVVNSIGISHLIEVIEQLNQNDGKLVFTNLDPTIEKTFSIMGLFQFADKA
                     DNVEAALST"
     gene            3335..>3772
                     /locus_tag="VF870_08775"
     CDS             3335..>3772
                     /locus_tag="VF870_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252322.1"
                     /translation="MENNNLVEKLSLQVESFQEGFEILSKSFSLVQMVKSFLHIVRGN
                     FIVSEIYAFHKINKTLNWNSISLQKIPDQSYLAYLQDSETPLIKYFKDQKIEVTITLP
                     LADHSYLGILIGQKLDKSEFTNLDKITLQILLQVFDSAYKSFLN"
BASE COUNT         1382 a          514 c          666 g         1210 t
ORIGIN      
        1 ggtctggaca aattagatta tgataaaaaa ttattcacta actattcgca caatgctgct
       61 aatccaaata gtctaataaa taattcagtt ttggtaatta cgggggataa atttggtgtt
      121 ttatggttag gtacgtttag cagtggaatg aattcttttg atccgaaaac aaattctttt
      181 aaacaattta taaacaaccc ctcagatcct aattcacttt cagataacag ggtttattct
      241 atattcgaag acagacaggg tactatttgg attgggactt atggcggagg tttaaatcga
      301 tttgacagat caactggaaa atttaaagta tataaacaca ttgaaaataa cagttcatca
      361 ataagttcaa acagtgtttg gacgattgct gaagataatg aaggtaaact ctggctagga
      421 acatttggcg gtggtgtaaa tatttttgat aaaaacaaac aaacctttac cagtttcagg
      481 aatgatccaa atgatcccac cacacttggt gatgataata taatacgggt ttttaaagat
      541 agcaaaaaca atatgtggct tggcacaaca aaaggattat gtcgtttcta tagtgataca
      601 aaaaagttta aaacatatcg tgagaaggat gggttagcaa atgattttgt ttatggaatt
      661 cttgaggacg ataaaggtaa tctttggtta agcacaaata atgggctttc aaagtttgat
      721 cccaaaaaag aatcatttaa aaattattat gttatagatg gtttgcaggg aaatgaattt
      781 aatcaaaatg cgtttgcaaa agattttaaa acaggcagat tactttttgg tggacctaat
      841 ggacttaata tttttaatcc agaggatctg aaggacaatg attacttgcc gccaattgct
      901 ttaacaaact atctgcgata taatactgat gatgaagaag gtaagccaat tattgaaaaa
      961 ggtatttcag aacgtgacag cattcttcta acttttaaag ataatattgt tactcttgaa
     1021 ttttctgctc ttagctattt taataattac cataatcaat acaagtacaa attagaaggc
     1081 tttaatgaaa attggattca gttagggaac aatcatacaa ttacttttac caatctatca
     1141 cctggggaat attatttaaa aattattgga tccaataatg acggtttatg gaatgagaat
     1201 ggtgctactt tatttataga agtacttccc ccttggtgga gaactaatat tgcttatacg
     1261 atatacggaa tcttattcgt gagcattttg tatggtttaa gaagatttga aatgaatcag
     1321 cataagcaga aagctcatat tcgcgagact gagttgaaga tgaaggcaac cgaagccgaa
     1381 aaacgtgctc tagaaattga gaatgaaaga aaaaccaaag agcttgaaga agcaagacag
     1441 ctgcagcttt ctatgcttcc taaaaaatta ccagttttac caaatcttga aattgctgct
     1501 tttatgcgaa ctgcaaccga ggtaggcgga gattattatg attttagagt tcttgaaaat
     1561 ggctctttaa atgttgcatt tggggacgca accggtcatg gacttcaggc aggtacaatg
     1621 gttactttga tgaaaggatt ttttacttcg gattcttcta aacttggcat gccggaattt
     1681 atgaatcact gcagtaaaat gataaaagag attgaattag gcagaatact gatgtcattc
     1741 agttatttaa aatttgaaga taagaaaata tatatcacaa gtgccggaat gcctcctatt
     1801 ttttattatg acaacaatgc aaacacagtt gaagaaataa ttattcaagg catgccgctt
     1861 ggagcaatga aaaaatttcc gtataaagta atagataaag aaattaaaag cggcgatact
     1921 atattattat taactgatgg attgcctgaa cagatgaata ataatgaaga aatgtttgat
     1981 tactctagag taaaaagcag ttttcatagt gttataaata attcccctga agaaataatt
     2041 aatagccttg ttaacgaagg cgataaatgg atgaatggca gagttcagga tgatgatatt
     2101 agttttgttg taataagaat taaataattt ttttatctct ctttaattct tagacaatat
     2161 tcaaaattat attatattcc aaaagttcta atatgttttt aataaatctt taattaatta
     2221 gtaagaaaga atttacatga aaaaaaatgt aaaactggaa atcaagttac ctaacattcc
     2281 tgatgttgaa ttggttgcta tcgaaagcct tcagattatg ggcaggcatt gcggtatttc
     2341 tgaagataaa atcggtgaag caaaaattat tgttacagag gctattatta atgcaattga
     2401 acattcaggt gaaaatagtc cttatgttct tattgagttc gatatggata ctgaaaagtt
     2461 gattatcctg gtaacagatt atggcacagg tttcgaacct gacagcgttg aagagccaaa
     2521 tattttagat aaaattcata gtccgcataa acgtggatgg gggcttaaac ttatgaaatc
     2581 catgtctgat gatttgatca ttgaatctgg accaaatggc actagaatta caattattaa
     2641 aaatttaata taaacggaga aatttaatga gcactgaatt taatttaatg tcagaattac
     2701 acgataaaac tcttgtaata aaaactgaag gatatattaa taatatcggc ggtgaaaaaa
     2761 ttgtccagga atttgggaag cacaatggag ttaatagact aattctaaac cttgaaaatt
     2821 ccaaagttgt aaactccatc ggcatttcac atttaattga agttattgaa caactgaatc
     2881 aaaacgatgg taaacttgtt tttaccaatc ttgatcctac aatagaaaaa acattttcaa
     2941 taatgggatt gtttcagttt gcagataaag cagacaatgt tgaagcagct ttaagtacct
     3001 gatcaaaaat tatgtgcaga aattgcatga taacaatgtt attgatgaca tttgattaat
     3061 ttcacctttc gtcaggatga acttagtaga atacagacaa tattaaaaat atctacaccg
     3121 ccactgcagt cggtgtgacg ttattataat tattaattaa aatagataaa caaaatcagg
     3181 tcgtcactaa ccaatcagtg gcaaaataat ttgtttttaa ttataagcta atatcaagtc
     3241 cggctgctag cgcgactgtt agtaaataaa attcataata cttttgaatt gagcatcaaa
     3301 agtatttatc cattcatttt aaaagataaa tattgtggaa aataacaacc ttgtagaaaa
     3361 gttatcacta caagttgaat catttcagga aggatttgaa atactttcta agtcattcag
     3421 cttagttcag atggtaaaaa gttttcttca catagtaaga ggaaatttta tcgtctcaga
     3481 gatttatgct tttcataaaa taaataaaac tttaaattgg aatagtatat cattacagaa
     3541 gattccggac cagtcttatt tagcatatct tcaagattct gaaactcctt taatcaagta
     3601 ttttaaggat caaaaaattg aggtaacaat tacattaccg cttgcagatc attcttattt
     3661 gggaattctt atcggtcaga aattagataa atcagaattc acaaatcttg ataaaattac
     3721 tttacaaata ctattgcagg tatttgatag tgcttacaag tcatttctaa at
//
LOCUS       DASWBY010000492        11308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_698224, whole genome shotgun sequence.
ACCESSION   DASWBY010000492 DASWBY010000000
VERSION     DASWBY010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698224"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VF870_08780"
     CDS             complement(<1..678)
                     /locus_tag="VF870_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX9252323.1"
                     /translation="MKEYILSKLKMNTNWYAIIALFIISGFCFQQSIAQTPARFYWKT
                     LSGANGVPLIVESINGNTNPFDPSHIVNPGAFVNATMAMVGYAKFLPLFDRSAMISVI
                     FPMGHISSEVTNGLTSKEDAKGFGDPMLEFNINIIGPKAQMNLVDLMRYEPGFSLDLM
                     MDIAFPVGEYDNTKTLNIGQNRWYGRLGAPIVWQIGPWVPGRRTTLEFLPAVWLFGDN
                     TDFVGKTM"
     gene            complement(726..2606)
                     /locus_tag="VF870_08785"
     CDS             complement(726..2606)
                     /locus_tag="VF870_08785"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX9252324.1"
                     /translation="MIDKLTISKFPDPQIHKSVKEKMPGSLNRVLSSLNLLLILLCLT
                     TIVGQSENFAQDSQRRPNIVIILCDDMGYSDLGSFGSEIQTPNFDNLAKEGTRFTNFY
                     THASCSPTRSMLLSGVDTHLNGLGNMNEWIAPNQTGVPGYEGHLNTQVATLPQLLKQS
                     GYHTYMVGKWHMGKSPELIPAARGFERDFSLLDGAGSYWDMTNFNATSPKSTYTEDGK
                     YLTELPGDYYATKTYTDKLIGYIEDNRKDGKPFFAYVSQQAPHDPYHLPRDWRNRHVG
                     EYDKGWDILRKERLNKQIELGIMPAGTQLAERMWFLPDPIVLAPAAKATLGKKMELYA
                     GMVENLDYHIGRLINYLKEIGEYENTIFIVFGDNGAEGNDLFKMIAGTPGTLNFLFAA
                     ANWSETDINAWGDPGTYVAYGPMWAQVSMTPFSQYKGWLGEGGIRNALIVSGPVVKRP
                     AGSINHGFMHVADIMPTLLEIANAKYPNKLEDGHELPPLIGKSWLPMLSGKTESPRTD
                     QDYMAWEVFGNRALVQGDWKIRWQFKSLGKEDWELFNIKTDPSERKDLAEEQPEKLSA
                     LLKLWDNYVKENNVIIPSRSPFETLYDQLPPRVPVEAGFPPLIYERQFIPPQDMMAEP
                     KK"
     gene            complement(2772..3047)
                     /locus_tag="VF870_08790"
     CDS             complement(2772..3047)
                     /locus_tag="VF870_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252325.1"
                     /translation="METVIINATINKNKKTEFFQTIESLKSLVKNYCNELETIISEDN
                     NLSIRITFSDHDEMENNFYNAEFNILKGSVMSLCKNVNIKINDVNLS"
     gene            complement(3225..4610)
                     /locus_tag="VF870_08795"
     CDS             complement(3225..4610)
                     /locus_tag="VF870_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9252326.1"
                     /translation="MITKLKISELNLIELIMDCMPGLVFFYAKDGKLVAWNKKAEEVL
                     EYPKEEFEAAFVGGFAVESDKEKVRNEFVNAFKNGFAEVEHRIKTKSGKEIPSLTKGI
                     VVRIESEEYLVGLSLDISELDNARKEILRLNELLQAENINLKEQLESTLEHNEIIGES
                     EWMKYIHFKIKQVAPTNASVLIEGETGTGKELIARAIHNESKRSNKPFIKVNCASIPE
                     NLIESELFGHEKGAFTGAIDKRIGRFELANGGTIFLDEIGELPLNVQPKLLHILQQGE
                     FERIGSSKTIKTDVRVISATNKVLEDEIKKGNFRNDLYYRLNVFPISVAPLRERKKDI
                     LPLIEFYIKKNSEKLNKPIKKFSDKSLQELIDYSWPGNVRELENVIERTIITSENDEL
                     NFETFTPVKNIANEFLPLEELEKNHIIKVLKKTSWKISGPGGAASYLNINPETLRSRM
                     RKLGINRPDLK"
     gene            complement(4650..6050)
                     /locus_tag="VF870_08800"
     CDS             complement(4650..6050)
                     /locus_tag="VF870_08800"
                     /inference="COORDINATES: protein motif:HMM:NF039990.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HEX9252327.1"
                     /translation="MKNNYYTSTYIFLMVLSFCLIIYPQQDTTITKTAIEKNIKDSIS
                     VDVPQLQDSSLTDTLINEAIQDSVEADTTLSQDSVKIKRNRNREKVYIDSTVTEDVHP
                     QDISDQAGFIIENLGKWASLRIYGSMRLNGAFDLNGLLNTNGFSTYDIPVGEDAIDQG
                     RFFMAPYQTRLGIEIKRSGLFNNFRIKIETDFLGANNGLRLRHAFIETGHFIIGQTWS
                     TFTDVNSVPQTVDIDGPNSSVQLRTVRIRYADRLLQSKLRYIVSLEAPSPDVVLPDSL
                     SDRVPAQFLPDIAGQVVRNFDFGYLSFSTILRGITVKDSSGSSEQILGYGFLLAGVYT
                     LDPDNKFLFQGVYGAAISRFITALNGKGIDLVYNPNTELFEATKSFGLMFSYTHEWRA
                     DISSAFTVGMVGINNKDYEPADAFSQSYYYSGNVFWNALLGVRLGLEIAQGRRINNDG
                     NWGRATRIGFIFYADF"
     gene            6280..6651
                     /locus_tag="VF870_08805"
     CDS             6280..6651
                     /locus_tag="VF870_08805"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX9252328.1"
                     /translation="MTQNFNKVSEKEKRRKLRRNQTFCENIVWRYLRNRNTLGYKFRR
                     QYSVDHYVIDFYCTKLKLAIELDGSVHDEPDQKEYDAYRQEYIEKFGITFCRITNDEL
                     MGNANMAFKKIEDAIKNLSKN"
     gene            6883..7524
                     /locus_tag="VF870_08810"
     CDS             6883..7524
                     /locus_tag="VF870_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX9252329.1"
                     /translation="MNRIIYTILILFLLQSISLFSQTYIGATGGLMSSSLSGDAPQDA
                     SYASKTGFSGGLLVDFTLTEDIVLSIQPRYLQKGSTIAYDVGEYELRDSLTATFDYIS
                     LPVMVKINSLNKRIYFSTGLDFGYLMNASVENIMDGSTKDVNNLIKSYDISATFGFGV
                     NIPIGSPIISLELRYMQSILNLSDISSAESGTTFPYRFRTSGFQFLTSIQFPI"
     gene            7563..8546
                     /locus_tag="VF870_08815"
     CDS             7563..8546
                     /locus_tag="VF870_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252330.1"
                     /translation="MKIKFFTIVYFAFSLIFISNAQSSDDKDQDTTETSLMNASGNVL
                     NGHRFISSSKIKSPFINTYLLTSIGAGKTGRLDFPIIINEQEITTLAGDLAFTYFGFE
                     YQHAVKDWIAFSLRINATGRIGTDAGAVLYQGLDLDVGYQFGWLLKLYKSRDMMLSGT
                     VKVANNSFTFIDINGFIQGIVDSGKITPENKLVINTPTADVGAGLAYAYTINRMFGIT
                     AEANFIYGESAERYSEDEWIFNYGILADADLNPDYSVPLGFGLSYYGSLRGKNTTSLL
                     GDPHNILFQINYTGKSDLDLGLVFNYQWYKDATIDQTINFTNVYMDVKYYF"
     gene            complement(8625..9272)
                     /locus_tag="VF870_08820"
     CDS             complement(8625..9272)
                     /locus_tag="VF870_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252331.1"
                     /translation="MNSVKVLSLKIFLVMFSTLLVFGCSQSNKITDSWVNPDATKESI
                     KFKKVAVFGLVMKTATRKLIEEQLARRLVNTIAVPSYKVVSDDDISKPEVVKAKLVEQ
                     GFDGALILRLVDIEKRESYSPGVYPSYYYSFGGFYGYSYGYMYNYTQDYGGQYMTDQI
                     VTAEIAIYSLSSDKLIWTGETMSMNPNDVEKTIAELSENVKEQLIDDGLLAKENF"
     gene            complement(9403..10098)
                     /locus_tag="VF870_08825"
     CDS             complement(9403..10098)
                     /locus_tag="VF870_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252332.1"
                     /translation="MKNILIIPVILLLLSNNAFSQKSYGSDLSATVAIPTGPNSEYFK
                     TGYGAIGGFYYEMESNWRFGLTLGFIRFGANSTELNNYFQTRLPVGTEGTLDIDGSVS
                     TIPILFSCKYVIPASASTKFYGIIEGGLYAYWTKAKGKIVYTLPTPGEVPFDESQFTS
                     EIGWDLGFGALFSVSENVSIDANFRYHFVRNSGTIKIKYDYNPYDTYGGEEAVGTSHF
                     LNIGIGANWNFDL"
     gene            10295..10765
                     /locus_tag="VF870_08830"
     CDS             10295..10765
                     /locus_tag="VF870_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252333.1"
                     /translation="MKNLMIVLLLGLFVSVNAQTEYFETDIRGIRTDIREVAKEIVAQ
                     NMELTPDQAKVFWPLYDEYMAELKVLGDREVKLTEEYMLNFYLMEEKTASQLLDDVLN
                     LEKDKLSLKSKYIGKMKEVLPAKVVGKFYQIERRLSLLIDAEKVSRIPLLKKEE"
BASE COUNT         3660 a         2038 c         1853 g         3757 t
ORIGIN      
        1 cattgtttta cccacgaaat cagtgttatc tccaaatagc cagactgccg gcaagaattc
       61 cagcgttgta cgtctgcccg gtacccacgg tccaatctgc cagacgatcg gggcacctaa
      121 tcttccgtac cagcgattct gtccgatatt taaagttttt gtattgtcgt attcgccaac
      181 cggaaaagct atgtccatca tcagatcaag tgagaatccc ggttcataac gcataagatc
      241 aaccagattc atttgcgctt tgggaccaat gatattaata ttgaattcga gcattgggtc
      301 gccaaaacct tttgcatctt ctttggaagt caatccgttt gtaacctcgc tggaaatgtg
      361 acccatagga aagatgactg aaatcattgc tgagcgatca aacaacggta aaaatttagc
      421 gtacccgacc attgccatag ttgcatttac aaaagctccc ggatttacaa tatgtgaagg
      481 gtcaaacgga ttagtattcc cgttaattga ttcaacaatc aaaggtacac cgttggcacc
      541 cgataatgtt ttccaataaa aacgagctgg agtctgtgca atactttgct gaaagcagaa
      601 cccgctaata atgaacaaag caataatggc gtaccaatta gtattcattt ttaattttga
      661 taatatatat tctttcatta tttcaccttc tatatttata aagtgttaat ttttccacca
      721 cggttctatt tctttggttc agccatcata tcctgtggcg gtataaactg tcgctcataa
      781 ataagcggcg gaaaacctgc ttcaaccgga acacgcggag gaagctgatc atacaacgtt
      841 tcaaacggcg aacggcttgg tattatgaca ttattctctt tgacataatt atcccaaagt
      901 ttgagcaaag cacttaactt ttcaggctgc tcttctgcaa gatcttttcg ttcactagga
      961 tcagtcttta tgttaaagag ttcccagtct tccttcccaa gtgatttaaa ttgccagcga
     1021 attttccaat ctccctgaac tagtgcacgg ttaccaaata cttcccatgc catataatcc
     1081 tgatcagttc gtggtgattc tgttttacct gataacatcg gcagccatga tttaccaatg
     1141 agcggaggca gttcatgtcc atcttctaat ttattcggat actttgcatt agcaatttca
     1201 agcaaggttg gcataatatc agcaacgtgc ataaatccgt ggttgatact tccggcgggt
     1261 ctttttacta caggcccact tacaatgaga gcgttacgta taccaccctc accaagccaa
     1321 cctttatact gactgaaagg cgtcattgaa acctgtgccc acatcggacc gtaagcaaca
     1381 taagttccgg gatcgcccca ggcattaata tcggtttctg accagtttgc tgcagcaaaa
     1441 agaaaattta gtgtgcccgg tgtaccggca atcattttaa aaagatcatt tccttcggct
     1501 ccgttatcac caaagacgat gaagatagta ttttcatact ctccgatttc tttaaggtaa
     1561 tttattaatc ttcctatgtg ataatccaaa ttctcaacca taccggcata tagttccatc
     1621 ttcttaccga gagttgcttt tgctgcagga gccaggacaa tcggatccgg aaggaaccac
     1681 attctttctg cgagctgagt accagcaggc ataataccaa gttcaatttg tttatttaat
     1741 ctttcctttc ttaaaatatc ccatccctta tcatattcac caacatgccg gttacgccaa
     1801 tccctcggaa gatgatatgg gtcgtgcggt gcctgttgag atacataagc aaaaaatggt
     1861 ttgccatctt ttctgttatc ctcgatatat ccaatcagct tgtcagtgta agttttagtt
     1921 gcgtagtaat caccaggaag ttctgtaaga tattttccgt cttcagtata agttgatttc
     1981 ggagaggtag cattaaagtt tgtcatatcc caataacttc ctgcaccatc cagaagcgag
     2041 aagtctcttt caaatcctct ggctgcagga ataagttccg gcgattttcc catatgccat
     2101 ttaccaacca tgtaagtgtg ataaccagat tgcttaagca gttgtggcaa agtagctacc
     2161 tgtgtgttca agtgaccttc ataaccgggc acaccagttt gatttggtgc aatccattca
     2221 ttcatattac caagaccgtt taagtgagta tcaactccac ttaacaacat tgatcttgtc
     2281 ggcgaacaac tggcatgagt atagaagtta gtaaagcgcg ttccttcttt tgccaggtta
     2341 tcaaaatttg gtgtctgtat ctcgctgcca aacgacccca aatcagagta tcccatatca
     2401 tcacaaagaa tgattacaat gtttggtctg cgctgtgagt cttgtgcaaa attttctgac
     2461 tgcccaacaa ttgtagtcag acataaaagt attaaaagta gatttaaaga acttaaaacc
     2521 cggtttaagc ttccaggcat tttttctttg actgacttat gtatttgagg atcaggaaat
     2581 tttgaaattg ttaatttatc aatcatgata tttatccttc ttttagtgga ttataaaatt
     2641 atctaaacaa acttttcata aaatactagc aggtaatagt tatggttcct taaagttttt
     2701 gattgttgat tatattttta attaaagtac ctaattttta aaattgaaat agttgataga
     2761 cattaaaatt tttagcttag atttacatca ttaattttaa tgttaacatt tttacataaa
     2821 ctcatcacag aacccttcaa aatgttgaac tcagcattgt aaaaattatt ttccatttca
     2881 tcatggtcgg agaaggttat tctgattgac aaattattat cctcactaat tattgtctca
     2941 agctcattgc aataattctt gactaaagat ttaagagatt caattgtctg aaagaattca
     3001 gtttttttat tcttattaat cgttgcatta attattactg tttccataaa taaactctct
     3061 agaatagaat tgtgttgaaa aatatttgag gataaatttg atttgctaaa ggcaattatt
     3121 aagccagagc agaatgaatt gattgtttta tttttggaag aaaattgaaa gattatgaat
     3181 atatcaatcg ttaaatataa cgtggccgtt aaatataacg agaattattt tagatctggt
     3241 ctgttgattc ccagcttacg cattctagaa cgcagagttt caggattgat atttaaatat
     3301 gatgccgcac cgccgggtcc gctgattttc caggatgttt ttttgagcac tttaataata
     3361 tgattcttct ccaactcctc cagaggtaaa aattcatttg ctatgttttt aactggagta
     3421 aaagtttcaa aattcaactc atcattttca gaagttatta ttgtccgttc aattacattt
     3481 tcaagttcac gaacatttcc aggccaggag taatcaataa gctcctgcaa agatttatca
     3541 gaaaattttt ttataggttt atttaatttt tcagaatttt ttttgatata gaattcaata
     3601 agaggcagta tatctttttt tctttctctt aaaggagcaa ctgatattgg gaacacattt
     3661 aacctgtaat aaagatcatt acggaaattt ccttttttga tttcatcttc aagaacttta
     3721 tttgttgcgg atataactct tacatctgtt ttaattgttt ttgaactgcc gatcctttca
     3781 aattctccct gctgcagaat gtggagaagt ttcggctgaa cattcaatgg taactcacca
     3841 atctcatcaa gaaaaattgt tccaccgttt gccaattcaa acctgccgat ccttttatca
     3901 attgcaccag tgaacgcacc tttttcgtgt ccaaacaatt cactttcaat taaattttca
     3961 ggaatggaag cacaattaac tttaataaat ggtttattac ttcttttgct ttcattatga
     4021 atcgctctgg caataagttc tttcccggta cctgtttcac cctcgataag aactgaggca
     4081 tttgtaggcg ctacttgttt gattttgaaa tgaatatatt tcatccattc gctctcacca
     4141 ataatttcat tgtgctctaa tgtagactct aactgctctt ttaaattaat attttctgcc
     4201 tgtagaagtt catttaatct caaaatttct ttacgtgcat tatccaattc agaaatatct
     4261 aaagataacc caactaaata ttcttcagat tctattctaa caacgattcc ttttgtaaga
     4321 gaggggatct ctttccctga tttagtttta attctatgct cgacttcagc aaaaccattt
     4381 ttaaaagcat tgacaaattc atttcttacc ttttctttat cactctcaac tgcaaatccg
     4441 ccaacaaatg cggcttcaaa ctcttccttt ggatattcca gtacttcctc agctttttta
     4501 ttccacgcga caagcttccc atctttggca tagaaaaaaa ccagaccagg catacagtcc
     4561 attatcaatt caatgaggtt caactctgat atttttaatt ttgtaatcat accttatttg
     4621 ataattaaat aattaggtta gaaatggaac taaaagtccg cataaaatat aaaaccaatt
     4681 ctggtagctc ttccccaatt gccgtcattg ttaatccttc tgccctgcgc aatttcaaga
     4741 ccaagtctta cacctaacaa tgcgttccaa aaaacgttac ctgaatagta atagctttgg
     4801 ctgaatgcat ctgcaggttc ataatcttta ttattaatac caaccatacc tactgtaaac
     4861 gcagaagaaa tatctgctct ccattcatgg gtatatgaga acattaaacc aaaagatttt
     4921 gttgcttcaa ataattcagt attaggatta taaactaaat ctattccttt gccgtttaga
     4981 gcagttatga atcttgatat tgctgctcca tagacaccct ggaagagaaa tttattgtcc
     5041 ggatcaagag tgtaaacacc cgccagtaaa aatccataac ccaatatctg ctctgaagat
     5101 ccggaagaat cttttactgt tattccacgt aaaattgtag aaaagcttaa atagccaaaa
     5161 tcaaaatttc ttactacctg accggcaata tcaggtaaga attgtgcagg tactctgtcc
     5221 gacaaagaat caggtagaac tacatcaggc gagggtgcct cgagagatac aatgtatctt
     5281 aatttacttt gcaagagtct atcagcatac cttatccgaa cggttcttaa ctgcaccgaa
     5341 ctgtttggac catctatatc cacagtttgc ggaacagaat taacatctgt aaatgttgac
     5401 caggtttgtc caataataaa atggccggtt tctataaaag catgacgcaa tcttaatcca
     5461 ttattagccc caagaaaatc tgtttcaatt tttattctaa aattattgaa taatccagac
     5521 ctctttattt caatacctaa tctggtttga taaggtgcca taaaaaatct gccctgatct
     5581 attgcatctt caccaacggg aatatcataa gtggaaaaac cattcgtatt taaaagcccg
     5641 ttcaggtcaa atgccccatt aagtctcatt gagccatata ttctaagaga ggcccactta
     5701 cctaaatttt caataatgaa tcctgcttga tcagaaatat cctgcgggtg aacatcttcg
     5761 gttacagtag aatctatata aaccttttct ctatttctat ttctttttat ttttactgaa
     5821 tcttgactta aggtagtatc tgcttcaaca gaatcttgaa ttgcttcatt aataagtgta
     5881 tctgtcaaag atgaatcctg cagctgagga acatcaactg aaattgaatc cttgatattt
     5941 ttctctatag cagtctttgt aattgtagta tcttgttgcg gataaatgat aagacagaaa
     6001 gataatacca ttagaaaaat ataagttgat gtatagtaat tatttttcaa aatcattctt
     6061 gtaataatat tgtagcaaag tatttataaa taacagttat aaattaacgg ttaataacgc
     6121 ttcttcaaaa tagaaataca cggaacaata tgagttcgtc ttctgccaaa aaataattaa
     6181 attcaatctc accctatttc cctctccttc taaaggagag ggagtttaaa gtccttctcc
     6241 tttgcaagga gaagggttgg gatgaggtag gaattaaata tgacacaaaa ttttaacaaa
     6301 gtatcagaaa aagaaaagag aagaaaacta agaagaaacc agactttttg tgaaaatatt
     6361 gtttggaggt atttaagaaa tagaaatact cttggttata aattcagacg tcagtactct
     6421 gtagatcact acgttataga tttttactgt acaaaactca aacttgcaat agaactcgat
     6481 ggaagcgtac acgatgaacc tgatcaaaaa gagtatgatg cttacagaca agaatacata
     6541 gaaaagtttg gaatcacttt ttgcaggatt acaaatgatg aacttatggg caatgcaaat
     6601 atggccttca agaaaattga agatgcaatt aagaacttaa gcaaaaacta atccatccct
     6661 acccttccct ttatcttaag gaaagagatt tatatacttc actttgctaa agagactgtc
     6721 ccgaacctta ttcgggagga ggtctaagga gaagttaatt aaagattacg cttaggactt
     6781 tgattaaaac aaaaatattt atgtcatctt atttgattta ataaaatcct catttaatat
     6841 tactgtagtt cttttattaa ataaagagtt taatgtaaaa ctatgaatcg aatcatttac
     6901 accattctaa ttttattcct cttacagagc atctcactat tttcacaaac ttacattggc
     6961 gcaactggcg gactaatgtc ttcaagcctt agcggtgatg cgccgcagga tgcaagttat
     7021 gcaagcaaga ccggattttc cggcggtttg cttgttgatt ttactctgac cgaggatatt
     7081 gtattgagta ttcagccaag atacctgcaa aagggatcca caattgctta tgatgttggt
     7141 gaatatgaat taagagatag tcttacagca acatttgatt atataagctt accggttatg
     7201 gtaaaaatta attctttaaa taaaagaata tatttttcaa ccggcctcga ttttggatat
     7261 ctgatgaatg catctgtaga aaatattatg gatggctcaa caaaagatgt aaataatctt
     7321 atcaagagtt atgatatttc agctactttt ggttttggag ttaacattcc gattggatct
     7381 cctattatct ctcttgaatt aagatatatg cagagtattt tgaaccttag tgatatatca
     7441 tcagccgaat ctggtactac tttcccttat agattcagaa catcgggctt tcaatttcta
     7501 acaagtatac aattcccgat ttaattaatt atccgttatg aaaatttttg aataaaatat
     7561 ttatgaagat caaatttttt acaattgtgt attttgcatt cagcttaatt ttcatttcta
     7621 atgcgcagtc ttctgatgat aaagatcagg atacaaccga aacatccctg atgaatgcaa
     7681 gtggaaatgt gctgaatgga catagattta tttcaagttc taagattaaa tctccgttta
     7741 taaatacata tctgcttacc agtattggag caggtaaaac aggtcgtttg gatttcccta
     7801 taattattaa tgagcaggag ataactacat tagccggtga tttagcattt acgtactttg
     7861 gttttgaata tcagcatgca gttaaagatt ggattgcgtt ttctttaaga ataaatgcta
     7921 ccggaaggat tggaacagac gcaggagcag tattatatca gggtcttgat cttgatgtag
     7981 gttaccagtt tggctggctg ttaaaattat acaagagtag agatatgatg ctctctggca
     8041 ctgtaaaagt tgctaataac tcttttactt ttatagatat taatggtttt atccagggta
     8101 ttgtagatag cgggaaaata acaccggaaa ataaattggt tataaatacg ccaacagcag
     8161 atgttggagc aggtttagct tatgcttata ctataaatag aatgttcggt atcactgctg
     8221 aagcaaattt tatttatggt gaatctgctg aaagatactc tgaagatgag tggatcttca
     8281 attatgggat tttagctgac gctgatttaa atcctgatta ttcagtaccg ttaggttttg
     8341 ggctttctta ttatgggagt ctaagaggaa agaatacaac aagtcttcta ggtgaccccc
     8401 acaatatttt atttcagatt aattataccg gcaagagcga tcttgatctt ggtctggtgt
     8461 ttaattatca gtggtataaa gatgcaacaa tagatcagac aataaatttt actaatgtct
     8521 atatggatgt aaaatattat ttctgatttg ataagaaata tttatctcta tctaacaaaa
     8581 aagcgatccg aagatcgctt tttttatagg aaattttata ataattaaaa attctctttt
     8641 gcaagtaaac cgtcatcaat caattgttct ttaacatttt cactaagttc agcaattgtc
     8701 ttttcaacat catttggatt catagacatt gtctcaccag tccaaataag tttgtcgctg
     8761 ctcaatgaat agatagcaat ttctgctgta acaatttggt ctgtcatata ctgaccgccg
     8821 taatcctggg tataattata catatagccg tatgaatatc cataaaatcc gccgaatgaa
     8881 taataatagc tgggatatac acccggacta tatgactctc ttttttcaat atcaaccaat
     8941 cttaaaataa gtgcgccgtc aaaaccctgt tctacaagct ttgctttaac aacttcgggt
     9001 ttggatatat catcatcgct gacaacttta taagaaggaa cggctattgt gtttacaagt
     9061 cgtcttgcta attgttcctc gatcaatttg cgggttgcgg ttttcattac tagcccaaaa
     9121 acagcaactt tcttaaactt aattgattct tttgttgcat cagggtttac ccagctgtca
     9181 gttatcttat ttgattgact gcagccgaaa accagcaatg ttgaaaacat cacaagaaat
     9241 atttttaacg ataaaacttt cactgaattc ataaacacct ttctattgta tcctttgtaa
     9301 attatattct aattgaataa ttaaataata actatctata agtcagataa ttaattagct
     9361 ctgttaagat ataaaaatat ctttaagtat ttagatgatt aattaaaggt caaaattcca
     9421 gttagctcca atgccaatgt ttaaaaagtg gctggttcca acggcttctt caccgccata
     9481 agtatcataa gggttataat catattttat ttttattgta cctgaatttc ttacaaaatg
     9541 gtacctgaag tttgcatcaa tcgatacatt ttcgcttaca gaaaataatg caccaaaacc
     9601 taaatcccag cctatctcgc tggtaaattg agattcatca aacggaactt caccgggagt
     9661 tggaagtgtg taaactattt tacctttagc tttcgtccaa taagcatata atccgccttc
     9721 aattatccca taaaattttg ttgatgccga agcaggtata acatatttgc agctgaatag
     9781 tataggtatt gtagagaccg agccgtctat atcaagagtc ccttcagttc ccaccggcag
     9841 acgagtttga aaataattat taagttcggt actgtttgct ccaaatctta taaaacctaa
     9901 agttaatcca aaccgccagt tggattccat ttcatagtaa aatccgccga tagcgccata
     9961 accggtttta aagtattctg aatttggacc ggttggtatt gcaactgttg cggacaggtc
    10021 acttccataa cttttctggc taaaagcatt attagaaagg agtaataaaa taacaggaat
    10081 gattaaaata tttttcattt agcaaattcc ttttcataat tttaattatc gatgtgcaaa
    10141 gctaattaaa atattctcga aaactcaaat taaaccagga cattttcata aatattaaat
    10201 ctgcaattgt ttaagttctc ataaagtttt tttatatatg gtaaacaaaa aaaacaatta
    10261 attatttata acttaaatta acaaaggaag tacaatgaaa aatttaatga tcgtcttatt
    10321 attaggacta tttgtttctg taaatgctca gacagaatat tttgaaacag atatacgcgg
    10381 tattagaact gatattagag aagtagcaaa agagatcgtt gctcaaaata tggaattaac
    10441 tccggatcaa gcaaaagtct tctggccatt atatgatgaa tatatggctg aattaaaagt
    10501 gttaggcgat cgagaagtca agttaactga agagtatatg ctcaattttt atctgatgga
    10561 agaaaaaacc gcaagccagc ttctggacga tgttcttaac ctggaaaaag ataaactatc
    10621 attaaaaagt aaatatatcg gaaaaatgaa agaagttcta cctgcaaaag tagttggcaa
    10681 attttatcag attgaaagaa gattatcatt gctaatagat gctgaaaaag ttagcagaat
    10741 acctttgctg aaaaaagaag aatagattac cacttactta aaaagtaagt catctaaaaa
    10801 aagttaccgc aggtttaagc ctgcggtgtt tttttttaaa ctcaacctga aaattgcagt
    10861 tacctaggta tatcatttct ctattcaatc tttaatggaa cggcagaata aacatttttt
    10921 cgagtcaacc ttccataatt tctcacacta cacaatccaa ttttacatcc gatatctaat
    10981 aaataataag atattcataa catatcaaat tataagaact tagactctta aaattaatca
    11041 gtattcttga gcattatagt atcttttaat ttaatatata aggatcaatg atttattaag
    11101 ctataagatt gggaatagcc tcatagaata attttattgt taagatttag tagtaatgag
    11161 aactgataaa taattacgtt atgtgggaag atattcttaa aaaaacaaat tggtgtaaat
    11221 aataaaaggg agaaatattc ttcctccctt ttctcaaata agaccattta ttttacagcg
    11281 tcactaaccg gctttttaac aagcggta
//
LOCUS       DASWBY010000493         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_22903, whole genome shotgun sequence.
ACCESSION   DASWBY010000493 DASWBY010000000
VERSION     DASWBY010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22903"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..434)
                     /locus_tag="VF870_08835"
     CDS             complement(60..434)
                     /locus_tag="VF870_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9252334.1"
                     /translation="MKVISFEDLQVWKDSRILVKSIYQLTANGKFNKDFGLREQIQRA
                     SVSIMNNIAEGFERNNNKEYIKFLGYSKGSAGEVRSMLYVATDLGYISQDSFNTYYQM
                     AINIITQLSNFIKYLKSSSIKK"
     gene            complement(495..1034)
                     /gene="ruvC"
                     /locus_tag="VF870_08840"
     CDS             complement(495..1034)
                     /gene="ruvC"
                     /locus_tag="VF870_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9252335.1"
                     /translation="MIILGIDPGTRITGYGVIKYLNNTFVRVAGGSINLPANEPIPQR
                     LEIIYSEISKIIKKYKPDEFAIETAFYGKNVQSAMKIGYARGVSILCALHNNVPASEY
                     SPREVKKSVVGKGAASKDQVSFMIKTILNLKSEKIKVDESDALAIALCHAFRMKTSVK
                     KTKDWKSFIEAYPERVIQN"
     gene            complement(1162..>1821)
                     /locus_tag="VF870_08845"
     CDS             complement(1162..>1821)
                     /locus_tag="VF870_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9252336.1"
                     /translation="GKIFTKLIKEITIAARDGGDPNGNPRLRLAVDNAKAANMPLDNI
                     ERAIKKATGELEGVTYHELTYEGYGPAGVAVLVEVATDNKNRTVAEVRHLFSKNGGSM
                     GETGSVAWMFDRKGVITLPAEGKKEDDVMEIILEAGADDLTTEEDYFEVTTTVESFET
                     VRRTLVDKNFAIENASLQWIAKNMIEVKGEDAEKVMKMIEGLEDLDDVQNVYSNADII
                     E"
BASE COUNT          551 a          340 c          245 g          685 t
ORIGIN      
        1 atcctatcat agttttttaa aattaaaaga ttgaaatatt caaagattca aaaattattt
       61 tattttttta tagaacttga ttttaagtat ttaataaagt tagaaagttg agtaataatg
      121 tttatagcca tttgatagta agtgttaaaa ctatcttgtg atatatagcc caaatcagtt
      181 gcaacataaa gcattgatct tacctcacca gctgatcctt ttgaataacc taaaaacttt
      241 atgtactctt tattgttatt tctttcaaaa ccttcagcaa tattattcat aattgaaact
      301 gaggctcttt gaatttgttc tcttaatccg aaatctttat taaactttcc attggcagta
      361 agttgataaa ttgattttac aagaattctg ctgtctttcc aaacttgtaa atcttcaaat
      421 gaaattacct tcatttaatc ctccaaagcc tttgaatatt tgtttgaatt ttaatctttg
      481 aattttttaa tctttcaatt ctgtataact ctttccggat acgcttcaat aaaagatttc
      541 caatcctttg ttttctttac agaagttttc attctgaaag cgtggcaaag tgcaattgca
      601 agcgcatcac tttcatcaac tttaatcttt tctgatttta agttaagtat tgtcttaatc
      661 ataaatgaaa cctgatcttt acttgcagca cctttaccca caacagattt tttaacttct
      721 cttggtgagt attcacttgc aggaacattg ttatgaagtg cacatagaat tgacactccg
      781 cgtgcataac caatcttcat tgctgattga acattttttc cataaaaagc tgtttcgatt
      841 gcaaattcat caggtttata tttttttatg attttgctta tttcactgta gataatttca
      901 agtcgttgtg gaattggttc attggcagga agattaatag atccgccagc aactcttacg
      961 aaggtattat ttaaatattt aataactcca taaccagtaa ttcttgttcc gggatctata
     1021 cctaaaatga tcatagaaaa gttttaagtt gatttaaaaa tagattccca cctcgacttc
     1081 gctcggtgtg acgttctaaa aattataata cagtctcttg cgtgggaata acaataataa
     1141 tattagtgat taagaataat attattcaat aatatcagca tttgaatata cattctgaac
     1201 atcatcaaga tcttcaagac cttcgatcat cttcataact ttttctgcat cttctccttt
     1261 tacttcaatc atattttttg cgatccactg gagtgaagca ttttcaatcg caaaattttt
     1321 atcaaccaaa gtccttctta cagtttcaaa agattcaacg gttgtggtaa cttcaaaata
     1381 atcttcttca gttgttaaat catctgctcc tgcttctaaa ataatttcca ttacatcatc
     1441 ttctttttta ccttctgcag gaagagttat tacacccttt ctatcgaaca tccaagcaac
     1501 tgaaccggtt tcacccatac tgccgccatt cttgctaaag agatgtctaa cttcagcaac
     1561 tgtcctgttt ttgttatcag tagcaacttc aacaagtact gcaactcctg caggaccgta
     1621 gccctcataa gtcaattcgt gataagttac accttccagt tcgccggtag cttttttaat
     1681 tgcacgttca atattatcta aaggcatgtt tgcagcttta gcattatcaa ccgctaatct
     1741 taatcttggg tttccattgg gatcaccgcc gtctctggct gcgattgtaa tttcttttat
     1801 aagtttggta aaaattttac c
//
LOCUS       DASWBY010000494         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_246638, whole genome shotgun sequence.
ACCESSION   DASWBY010000494 DASWBY010000000
VERSION     DASWBY010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246638"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VF870_08850"
     CDS             <1..303
                     /locus_tag="VF870_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HEX9252337.1"
                     /translation="RIAKYLKPWHVVAFWIGFTFDVSGTYAMHLIAEGPFDILEPHTL
                     TGQLALWLMLIHAVWATRVIIKQDEALKVKFHKFSIVVWFIWLIPYFGGMFLGMSK"
     gene            complement(491..1570)
                     /locus_tag="VF870_08855"
     CDS             complement(491..1570)
                     /locus_tag="VF870_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252338.1"
                     /translation="MNKLILIFLFFMLILTITESSALPRYAVKLGDKCSSCHYNPSGG
                     GMRSLDGWYWGKNTLSMISARDKDFLMSPKISDNISMGLDYRTQYIYSQSSGRTYFQN
                     MTAALYTNFALSKQINLVGKYDFVNELWEGYGVAKILPGGGYLKAGVYTPNFGIRIDD
                     HTAYTREGLIFNPFYREAGVEIGYFVDDWLYASASAGSNLSDVTSNYTKDPTYTARAE
                     LTPRFGEIGFIIGGSYAAVKLQGSEGTLNTNMYGGFFGIGYDEFSLLAEYDMANDLSV
                     AGVKSNMLMVEAAYGIITGLDAVVRYDRIDKNADVDSDEINRIIMGFEFHPYSFIEVR
                     PQYRLVLEDPSVDNDSFVLQLHFWY"
     gene            1846..2763
                     /locus_tag="VF870_08860"
     CDS             1846..2763
                     /locus_tag="VF870_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9252339.1"
                     /translation="MVLKNTKNKNLTRFAWLSVAAAILTISLKGIAYLLTGSVGLLSD
                     ALESVVNLVGALMALAMLTIAARPADEDHAYGHSKAEYFSSGVEGMLILIAALGIAVT
                     AIPRLITPKPIEQIGLGLTVSIVASIINLIVSIILSRAGKKYNSITLEADAQHLMTDV
                     WTSAGVFAGVGAVALTGWERLDPIVAILVAGNIVWSGVRIMRKSTFGLMDAALSPEEQ
                     KIIQSVLEKFLQDGVQYHALRTRQSGVRKFVSLHILVPGEWTVQRSHRLLENIESSIR
                     HSLPGVTVFTHLESLDDPASWEDIDLDRN"
     gene            complement(2821..>3177)
                     /locus_tag="VF870_08865"
     CDS             complement(2821..>3177)
                     /locus_tag="VF870_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252340.1"
                     /translation="AVKAIEKDSSDYLPYIILSIYNRQIASLNWFERAFANTFFGKVP
                     DGSLKESERLMLKSLKLEPGIVTAMYQLSLTYKEMGNEKKEIEWLKKVLDAPITDFRD
                     KYAKRKAKHRLSELNN"
BASE COUNT          946 a          618 c          557 g         1056 t
ORIGIN      
        1 agaatagcaa aatacctgaa accctggcac gttgtggcgt tctggattgg ttttacattt
       61 gatgtttcag gaacttatgc aatgcatctt atagcagaag gaccatttga catcttagag
      121 ccgcacacat taacaggtca acttgctcta tggttgatgc tgatacacgc cgtctgggca
      181 acaagagtaa ttataaaaca agacgaagca ttaaaagtga aatttcacaa gttcagtatc
      241 gtagtctggt ttatatggtt gataccttac tttggtggaa tgtttttggg aatgagcaaa
      301 taatatttat tttatataaa tagtttttcc gaagtgccca atatttgtag ccattggcca
      361 atccagttta aggtttgtat gattaacttt ttaccagaaa tacagtttct tgtttcttca
      421 tacaattatt cggctcgtac cctcgcacgt cccttactaa ctcattttgt tgcgtcaacc
      481 accggcttta ttaataccaa aagtgtaatt gaagaacgaa cgaatcatta tcaactgaag
      541 gatcttctaa aaccaatcta tattgtggtc gaacctcaat aaaactgtat gggtgaaatt
      601 caaaacccat aattattcga ttgatctcat ctgaatcaac atctgcattt ttatcgattc
      661 tatcatatct gaccactgca tcaagccccg ttataattcc gtaagcagct tctaccataa
      721 gcatattcga ttttacaccg gcaacagaaa gatcattcgc catatcatat tcggcaagta
      781 atgaaaattc atcataaccg ataccaaaaa atccaccgta catgtttgta tttaacgttc
      841 cttcacttcc ttgaagtttt actgctgcgt atgaacctcc aattataaaa ccaatttccc
      901 caaagcgagg tgtcaattcc gctcgtgcag tgtaggttgg atcttttgta tagttgcttg
      961 taacatctga tagatttgag cctgcacttg cacttgcata aagccaatca tccacaaaat
     1021 aaccgatctc aaccccggct tctctataaa acggattgaa aattaatcct tctcttgtgt
     1081 aagcagtgtg gtcgtctatt ctaataccaa aatttggtgt gtatacccct gctttcaagt
     1141 aaccacctcc gggcagtatt tttgcaacac cgtaaccttc ccacaattca tttacaaaat
     1201 catacttccc aacaagatta atttgttttg ataatgcaaa gtttgtgtaa agtgctgcag
     1261 tcatattctg gaaataagtt ctgccgcttg attgcgagta tatatattgt gttctgtaat
     1321 caagtcccat tgaaatatta tccgaaattt ttggtgacat aagaaaatcc ttatcacgcg
     1381 cagagatcat acttaaagta tttttacccc agtaccatcc atctaaactt ctcattccac
     1441 caccgcttgg attgtaatga cagctgctgc atttatcgcc aagtttgact gcatatcttg
     1501 gcaacgcact cgattctgtt atcgttaaaa tcaacataaa gaataaaaat attagaatta
     1561 atttgttcat atctttccaa cgttaattaa ttaaattaaa atctcataga gttattgtaa
     1621 cctaaaaata aaataatgca tcaactattt aagtagtttc cattatgaac agatagtgac
     1681 atttaaatta aaaaattttt aattttttta atttatgtac tttataataa acaggtattg
     1741 tgatttatta caatacttca tttggaacag gaattttcct gaaatggtgg caatcatata
     1801 ttttttagtc aattttgatc ctcaaactaa tgcagaaatc aaactatggt attaaaaaat
     1861 acaaagaata aaaatcttac acgctttgcc tggctttcag ttgcggccgc tattcttact
     1921 atttcgctta aaggcatcgc ctatctgctt acaggctcag tcgggctgct ctcagatgct
     1981 ctagaatccg ttgtcaacct ggtgggggca cttatggcgt tggcaatgtt gactatagcg
     2041 gcccgtcctg cagacgagga tcacgcatat gggcatagta aggcagaata tttctccagc
     2101 ggtgtcgaag gtatgttgat actaattgca gccttgggta tagccgtaac tgccattccc
     2161 agacttatta ctccaaaacc aattgaacag atcggtttgg gattgacagt ttcgattgta
     2221 gcttctatta taaatttgat tgtttcgatt atcctgtcaa gagctggcaa aaaatataat
     2281 tcaataactc ttgaagcaga tgctcaacac cttatgactg atgtctggac atcagccggt
     2341 gtttttgctg gggttggtgc agtagcactg actggatggg aaaggttaga tcctattgtt
     2401 gcaattcttg ttgcgggaaa tattgtttgg tcaggcgttc gtattatgcg taagtcaacg
     2461 tttggattga tggatgccgc tctttcgcca gaggaacaaa aaattataca aagtgtgttg
     2521 gaaaaatttt tacaggatgg tgtacaatac cacgcacttc gaacccgaca gtctggggtc
     2581 cgcaagtttg tttcactgca catacttgta cccggtgaat ggacagtcca acgaagtcat
     2641 agattgctgg aaaatattga gtccagtata agacattcgc ttccgggtgt tactgttttt
     2701 acacatcttg aatctttaga cgacccggca tcctgggaag atatagatct tgaccgcaac
     2761 taaactctgc tataaccaga acgaaatcac aatgttaatc ggattatttt actgtgtttt
     2821 ttaattattt aattctgata atctgtgttt tgctttcctt tttgcatatt tatctctgaa
     2881 atcagttatt ggtgcatcaa gtactttttt aagccactca atttcttttt tctcattgcc
     2941 catttcttta tatgtaagtg aaagttgata catagcagtc actattccag gctcaagctt
     3001 taaagatttt aacattaatc gttctgattc tttaaggctt ccatctggca cttttccaaa
     3061 aaatgtattt gcaaatgcac gttcaaacca atttagactt gcaatttggc ggttataaat
     3121 acttaaaatt atgtatggta ggtaatctga ggaatctttc tcaatcgctt taacagc
//
LOCUS       DASWBY010000495         7453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_133238, whole genome shotgun sequence.
ACCESSION   DASWBY010000495 DASWBY010000000
VERSION     DASWBY010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133238"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VF870_08870"
     CDS             <1..419
                     /locus_tag="VF870_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252341.1"
                     /translation="FEDNVLSFYAIDDTVRKFISIESIDKKLIINTFNRFYKIYSELM
                     NEEQEPMLNLVSFYEEEYGWRVIIFLRAKHRPAVFFSEDENKMLVSPAAIDLGGVCVF
                     PRQEDFNRITKEQVTEIFKEVFIDENKLKSLAVKLS"
     gene            complement(592..2802)
                     /gene="katG"
                     /locus_tag="VF870_08875"
     CDS             complement(592..2802)
                     /gene="katG"
                     /locus_tag="VF870_08875"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX9252342.1"
                     /translation="MSDESKCPVPHGANTRNAGRGTSNRDWWPNQLNLGILHQHHPAS
                     NPMGPDFDYAKEFKKLDLKALKKDLYELMTTSQDWWPADWGHYGGLFIRMAWHSAGTY
                     RTADGRGGGGTGNQRFAPINSWPDNGNLDKARRLLWPIKQKYGNKISWADLFILAGNC
                     ALESMGFKTFGFGGGRADIWQPEEDIYWGAEREWLATSDKPKSRYSGDRELENPLAAV
                     QMGLIYVNPEGPDGNPDPVASGRDVRETFKRMAMNDEETVALTAGGHTFGKMHGAGDP
                     KLVGPEPEAAPIEEQGLGWINKFGTGKGVHTTTSGLEGAWKPNPTKWDMGYFDMLFGY
                     EWEKVKSPAGAWQWQAKDVKPEHMIPDAHDPSKKHPPTMTTADMSLRFDPIYEPISRK
                     FHKDPKAFADAFARAWFKLTHRDMGPKARYLGPEVPKADLIWQDPIPAVNHKLINKKD
                     IADLKKKILDSGLSVSELVYTAWSSASTFRGSDMRGGANGARIRLEPQKDWEVNQPAQ
                     LEKVIKKLKSIQKHFNNAQKDGKKVSLADLIVLGGCAAIEEAAKAAGFKIEVPFSPGR
                     MDASQAQTDVESFSVMEPEADGFRNYQKKAYTISVEEMLVDKAQLLTLSAPEMTVLVG
                     GMRVLGANFGGSEHGVFTDQVGKLTNDFFVNLLDMGIVWKPISEAGDAFEGRDRKTGK
                     QKWTGTRVDLIFGSNSQLRALAEVYAQDDAKEKFVNDFVAAWNKVMNLDRFDLK"
     gene            complement(2844..3269)
                     /locus_tag="VF870_08880"
     CDS             complement(2844..3269)
                     /locus_tag="VF870_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX9252343.1"
                     /translation="MRKEKHGIDLKNGNLKVTPQRVAVLEALSNLNDHPTADKIKEYV
                     VKNHPNIAVGTIYKTLETFVEKGFVKKVKTDEDVMRYDAILDKHHHLYCEDTNRIEDF
                     FDDELNSMLEYYFKRKRIANFKVKDIKLQIIGTFNNKKN"
     gene            complement(3517..4668)
                     /locus_tag="VF870_08885"
     CDS             complement(3517..4668)
                     /locus_tag="VF870_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9252344.1"
                     /translation="MAQKRNTDSTTKFFITIIGLVVITIVLKELSSIFIPLVIAIFLF
                     FVFSPLNAWLSNKKVPMYVITLLDLVIIFGLLYGVGRIVVDSFLQFSEGLPIYGDKLS
                     NIVRAAATSWGVTDPFFTQFSFDQLLKMLNLQNLAGGIFSSTVSLLGSVLFVLFFFLF
                     VLSGNETIYKSIKHRYVFKKVSPELKRIKKNLNQSENSADEQKLLDNFQHERQLREQQ
                     LELTFKKITEQIQRYIIAKVGINLIAGIITTIVLSLLGVDFPIVWGLFVFLFNFIPTI
                     GSAAALVFPVLFTLVQFNSIGTMLLVLVLMAVIQTLAFNVAEPMILGKRLNLNPLLIL
                     LSVLVWGYIWGIVGMLLSVPLTAIIKIIISNSTSPNMEFFEHLMSQEKE"
     gene            complement(4765..6093)
                     /locus_tag="VF870_08890"
     CDS             complement(4765..6093)
                     /locus_tag="VF870_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252345.1"
                     /translation="MNKIISILVLAVILISCSEKKEPIIQSDLELKMNSIAENYVKLV
                     LNIGQYDADFVDAYYGPKEWRDSLKTDLPFDSTTYKNLSVKTNELLNELESLGNYKAD
                     ELETLRFRYLYKQLLACKTKIYMLNGVLLPFDEETKALYDASAPIRKDEYFQSAIAEL
                     DKLLPGKGDIANRLNDFKMKFVIPTDKLKNVFDAAIKECRTKTLNHIKLPEEESFMVE
                     YVKDKPWGAYNWYKGNYFSVIQVNTDLPIYIDRAVDLAAHEGYPGHHVYNVLLESNLA
                     KKRNWDEFKVYALFSPQSLIAEGTANYGIEVAFPGDERIKFEKEVLFPLAGLNPTEAD
                     LYYKVLDIQKLLSYAGNEAARNYLDGKWTREQTVEWLQKNQLRTKDSADKYVSFIEKY
                     RSYVINYNLGMDIVKDYIERNGGTEKNKGKRWELFEHLLSTPQTPSGLVK"
     gene            complement(6086..6463)
                     /locus_tag="VF870_08895"
     CDS             complement(6086..6463)
                     /locus_tag="VF870_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252346.1"
                     /translation="MAKLTIAYGVIFIIMGLYGYFGISSESITALIPTFFGIPILVFG
                     WLGFNEKYLKHAMHGAAVLMLVGFAGTVGGLIKFFKMLGGSETARPAAVTVQAIMAVL
                     CLVFLILAVKSFIDARKNRTKSE"
     gene            complement(6472..>7453)
                     /locus_tag="VF870_08900"
     CDS             complement(6472..>7453)
                     /locus_tag="VF870_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9252347.1"
                     /translation="PSFDFVGSYEALEFMTDNGVWWLKVTGADGYRHDAVKHVPNEFW
                     RLLTKKIKCEIAIPENKTIYQIGESFGGIDMIASYVNNGQLSAQFNFNLYDVAVPTFL
                     DEKASFKLLDYQMQKSFQVFGYNNLMGNIMDSHDKIRYMAYADGDLAINDGRAGDFAW
                     NNPLKVDDPKSYDKLELHLAYILTIPGIPIIYYGDEIGMTGAADPDNRRMMRFDDELN
                     QYEKQTFNDVSKLIHIRKENSALRYGDFLTLQADENIYAFLRSDMNERILVVVNKNSN
                     EQKLDFTLPGMYNITKANDLLTGKEFDVNNNRISFSVGGTGYLILKLEKQ"
BASE COUNT         2302 a         1460 c         1216 g         2475 t
ORIGIN      
        1 tatttgagga taatgtttta tctttttatg caattgatga tacagtgaga aaatttattt
       61 caatagaaag cattgataaa aaattaatta taaacacatt taataggttt tataaaattt
      121 attctgaatt gatgaatgaa gagcaggagc caatgctgaa tcttgtttct ttttatgaag
      181 aagaatatgg ctggcgtgta ataatatttc taagagcaaa acatcgtcct gctgttttct
      241 tttcagagga tgaaaacaaa atgttagtaa gtcctgcagc aatagattta ggcggcgttt
      301 gtgtttttcc gcgccaagaa gattttaatc gcattacaaa ggaacaggtt acagaaatat
      361 ttaaagaagt atttattgat gagaacaaat tgaaaagtct ggcagtaaaa ttaagctaat
      421 gctgcctacc aatcctgcaa cttgatttat aaaacaaaca ttatctgaaa ttcactacta
      481 atattatgct catcataaaa aaaggggcat cgtggtgatg ccccttctaa gcttagagtt
      541 tgggatttcc cgtccacaat ttatctggac tcagaatgac agacgctttg tttactttaa
      601 atcaaaacga tcgagattca tcactttgtt ccaggcggca acaaaatcat taacaaattt
      661 ttcttttgca tcatcctgtg cataaacttc cgccaatgca cgtaattggg aattcgatcc
      721 aaagattaaa tcaactcgtg tacctgtcca tttttgtttt cctgtcttgc gatcgcgtcc
      781 ttcaaatgca tcacctgctt cggaaattgg tttccaaact attcccatat caagcagatt
      841 aacaaagaag tcatttgtta gtttaccaac ctgatctgta aacacaccgt gttcagaacc
      901 accaaagttt gcgcctaaga ctcgcatacc accaacaaga acagtcattt ctggtgcact
      961 tagtgtcagg agctgtgctt tatcaacaag catctcttca actgaaatgg tataagcttt
     1021 cttctgataa tttcggaagc catcagcttc gggttccatt acagagaatg attccacatc
     1081 agtctgagct tgtgaagcat ccattcgacc cggtgaaaat ggcacttcaa ttttaaatcc
     1141 tgcagcttta gctgcttctt caattgcagc acatccacca agcacaatca gatcagcaag
     1201 ggaaactttt ttaccgtctt tctgtgcatt gttgaaatgt ttttgaatgc tcttaagttt
     1261 tttaattact ttttccagtt gggctggttg gttaacttcc caatcctttt gcggctctaa
     1321 acggatacgc gcaccatttg cgcctccgcg catatccgat ccacgaaaag ttgatgccga
     1381 agaccatgca gtgtaaacaa gttcggaaac agaaagtcct gaatcgagga ttttcttttt
     1441 tagatctgca atatcttttt tattaataag tttatgattt actgctggga ttggatcctg
     1501 ccatattaaa tctgcttttg gaacttctgg tccaaggtaa cgagctttcg gtcccatatc
     1561 tcggtgtgtt aacttaaacc atgctcttgc aaaagcatca gcaaaagctt tcgggtcttt
     1621 gtggaatttt cgtgaaatcg gttcataaat aggatcgaag cgtaaagaca tatctgcggt
     1681 tgtcatcgta ggtggatgtt tctttgatgg atcgtgtgca tcgggaatca tatgttccgg
     1741 cttaacatct tttgcctgcc attgccatgc accagcagga cttttaacct tttcccactc
     1801 atagccgaaa agcatatcaa agtagcccat atcccatttt gttggatttg gtttccatgc
     1861 accttccaaa ccgcttgttg ttgtgtgaac accttttcct gttccgaatt tatttatcca
     1921 tccaagtcct tgctcttcaa ttggagcagc ttccggttcc ggaccaacta actttggatc
     1981 accagcgccg tgcatcttac caaatgtatg tccacctgcg gtaagcgcca cggtttcttc
     2041 gtcattcatt gccatacgtt taaatgtttc gcgcacatca cgacctgatg ctacaggatc
     2101 aggattacca tcagggcctt cggggtttac ataaatcagg cccatctgaa ctgctgctaa
     2161 aggattttcc agttcgcgat cacctgagta acggctttta ggtttatcac ttgtggcaag
     2221 ccattctctt tcagcacccc aatatatgtc ttcttctggc tgccagatat cagcacgtcc
     2281 gccaccaaaa ccaaatgttt taaaacccat tgattccagt gcacaattac ctgctaagat
     2341 aaaaagatct gcccaggaaa ttttattacc atatttctgt ttgattggcc aaagaagacg
     2401 acgagcctta tccagatttc cgttatccgg ccaactgttg ataggtgcaa aacgctggtt
     2461 gccggttcca ccgccaccac gtccatcagc agtacgatag gtacctgcgc tatgccatgc
     2521 catacgaata aacagaccac cgtaatgacc ccaatctgcg ggccaccaat cctgtgaggt
     2581 agtcatcaat tcatacaagt cttttttcaa tgctttcaga tcaagcttct tgaattcttt
     2641 agcgtaatcg aaatccggac ccatagggtt ggatgccgga tgatgctggt gtagtattcc
     2701 aagatttaac tggttaggcc accaatcacg atttgatgtg cctctgcctg cattgcgggt
     2761 gttagctcca tgcggtacag gacacttgct ttcatcactc ataaatattc tcctttagtt
     2821 tttatagtta ataaattatt cgattaattc tttttgttat taaaagttcc aattatctga
     2881 agtttaatat cttttacttt aaagttggcg attctctttc tcttaaaata atactcaagc
     2941 atactattaa gttcgtcgtc aaaaaaatct tctatgcggt tagtgtcttc gcaatataaa
     3001 tgatgatgtt tatcgagtat tgcatcataa cgcataacat cttcatcggt ttttactttt
     3061 ttaacaaatc ctttttctac gaatgtttca agtgttttat aaatcgtacc aactgcaatg
     3121 tttggatgat ttttaaccac atattctttg atcttatcag cagtgggatg atcgtttaaa
     3181 ttgcttaagg cttccaaaac agcaactctt tggggcgtaa cttttaaatt accgttcttt
     3241 aagtcgattc cgtgtttttc ttttctcata gttaaatctt attaaatagg aattattctt
     3301 acttagtata catgtatatt tctaaaattt caagttcaat ttttattaca taaatattcc
     3361 ctttttatat aactgtggaa taacatatat aaaatttaga aagtcaaaat tgttgttaag
     3421 gaaggatgcc taatataagc gatagctcac aaatttagct ataaaacccg atagataatt
     3481 ctataatggc tgcattttaa gaaatataac ctatgtctat tctttttcct gactcatcaa
     3541 atgttcaaaa aattccatat ttggtgaagt tgaatttgaa attataattt ttattattgc
     3601 tgtaagaggt acagaaagaa gcattccaac aattccccag atataacccc ataccagaac
     3661 agaaaggaga attaataaag gatttaagtt taatcttttt ccgagaatca ttggttcagc
     3721 aacattaaat gcaagtgttt ggataacagc cattagaaca agtacaagca gcattgttcc
     3781 aatagaatta aattgaacaa gtgtgaacaa aacagggaag actaaagcag cggcagagcc
     3841 aattgttggg atgaaattaa aaagaaaaac aaataatccc caaactattg gaaaatcaac
     3901 accgaggagt gataaaacta ttgttgttat aatacctgca ataagattaa taccaacctt
     3961 tgcaataata taacgttgaa tttgctcggt tattttctta aaggtcaatt caagctgctg
     4021 ttcacgtaat tgtctttcgt gttgaaaatt atctaaaagt ttttgttcat ctgcagagtt
     4081 ttcagactga ttcaaattct tttttattct ttttaattcg ggactgactt ttttaaaaac
     4141 gtatctatgt ttaattgatt tataaattgt ttcgttacct gagaggacaa ataagaaaaa
     4201 gaacaacaca aataatacac tgccaagcaa tgaaacagta gaggaaaaaa ttcctcccgc
     4261 cagattttgc agatttaaca ttttcagcag ctggtcaaaa gaaaactgtg taaaaaatgg
     4321 atctgtgact ccccaggaag tagctgcagc tcttactata ttgcttagtt tatcgccata
     4381 tattggtaag ccctcgctaa actgaagaaa agaatcaaca actattcttc ctactccata
     4441 taataggcca aatattatta caagatcgag taatgttata acatacattg gcactttctt
     4501 gttgcttaac caggcgttaa gcggagaaaa aacaaagaat agaaaaattg caattaccag
     4561 aggaataaaa atgctgctaa gttcttttag tacgatagta attacaacta atccaataat
     4621 tgtgatgaag aatttagttg tgctgtctgt atttcttttt tgtgccatga tgtttattat
     4681 taaataatta tgaacttgtt tcggagccta aataattact agagaacccg aaacaatccg
     4741 ccaaaggcgg acagtttgaa aaacttattt tactaatcca cttggagttt gtggtgttga
     4801 taataaatgc tcaaacaatt cccatcgttt gcctttattt ttttcagtac cgccgtttcg
     4861 ttctatataa tctttaacta tgtccattcc taaattataa ttgatgacat aacttctgta
     4921 cttttcaata aacgaaacat atttatccgc actatctttt gtgcgtaatt gattcttctg
     4981 aagccattca actgtttgtt cacgtgtcca tttcccatca agataatttc ttgcagcttc
     5041 atttcccgca tagcttaaaa gtttttgtat gtctaatact ttgtagtaaa gatcagcttc
     5101 agtaggattt aatccggcaa ggggaaacaa tacttctttt tcaaatttta ttctttcatc
     5161 ccccggaaaa gctacttcaa taccataatt agcagttcct tcagcaataa gtgattgagg
     5221 agaaaataat gcataaactt taaattcatc ccaattacgc ttttttgcaa gattagattc
     5281 cagcagtaca ttataaacat gatggcccgg atagccttcg tgggcagcaa gatcaactgc
     5341 tcgatctata taaattggca aatcggtgtt tacttgtata acactaaaat aatttccctt
     5401 gtaccagtta tatgcacccc acggtttatc tttaacatat tcaaccataa aggattcttc
     5461 ttccggcagt tttatatgat ttaatgtttt tgttctgcat tctttaattg ccgcatcaaa
     5521 aacattttta agcttatcag ttggaataac aaacttcatt ttaaaatcat ttaatcggtt
     5581 tgcaatatca cctttaccgg gaagaagttt atcgagctct gcaattgctg attgaaaata
     5641 ttcgtctttg cgtattggtg cggaagcatc atataaagct ttcgtttctt catcaaaggg
     5701 aaggagcaca ccgtttaaca tatatatttt tgttttacag gcaagaagtt gtttgtaaag
     5761 atatctgaac cggagtgttt ctaattcatc tgctttgtaa tttccaagcg attcaagctc
     5821 atttaaaagt tcattggtct ttacagataa atttttatat gttgttgaat cgaacggcaa
     5881 atctgttttt aaactatccc gccattcttt gggtccgtaa taggcatcaa caaaatcagc
     5941 atcatattgc ccgatgttta aaacaagttt tacataattt tctgctatac tattcatttt
     6001 caactcaaga tcgctttgaa ttattggttc ttttttttcg ctgcatgaaa ttaaaattac
     6061 agctaatact agaatggaaa ttatcttatt cactttttgt cctgtttttt cgagcatcaa
     6121 taaatgattt aactgctaaa attaaaaaca ccaaacaaag aacagccatt attgcctgaa
     6181 cagtaactgc ggcaggtctg gctgtttcag atccaccaag cattttaaag aattttatta
     6241 atccgccgac tgtacctgca aacccaacta acattaaaac agcagctccg tgcattgcgt
     6301 gctttaaata tttttcatta aatcccagcc agccaaaaac taaaatcgga attccgaaga
     6361 aagttggaat aagtgcagtt atactttcac tgcttattcc aaagtaacca tacaaaccca
     6421 ttattataaa tataacacca taagcgatag ttaatttagc cattgtatct cctattgttt
     6481 ttctaatttc aaaattaaat aaccagttcc gccaacgcta aaagaaatcc tattattatt
     6541 cacatcaaac tctttccctg ttaaaagatc attcgctttt gtgatgttgt acattccggg
     6601 caaagtaaaa tctaattttt gttcgttgga atttttattt acaacaacaa gaattctttc
     6661 attcatatca gatcttaaaa aagcataaat attctcatct gcctgcaggg ttaaaaaatc
     6721 tccgtagcgt aaagctgaat tttcttttct gatgtgaatc agtttactta catcattaaa
     6781 tgtttgcttc tcatattgat taagctcgtc atcaaaacgc atcatccttc tgttatccgg
     6841 atcagctgcg ccagtcattc caatttcatc accataataa ataattggaa taccaggaat
     6901 ggttaatatg taagcaagat gtaattctaa tttatcataa cttttaggat catcaacctt
     6961 gagtggattg ttccaggcaa aatcacctgc tctgccatca ttaatagcaa gatcgccatc
     7021 ggcatacgcc atataacgga ttttgtcatg actatccata atgttgccca tcagattatt
     7081 gtaaccaaaa acctgaaaac ttttctgcat ctgatagtca agcaatttga acgaagcttt
     7141 ttcatcaaga aatgttggaa cagcaacatc ataaagatta aaattaaatt gcgcacttaa
     7201 ctgtccgtta ttaacataag aggcaatcat atcaattccc ccgaacgatt cacctatctg
     7261 ataaatcgtt ttattttcag gaattgctat ttcacattta attttttttg tgagcaatct
     7321 ccagaattca tttggaacat gtttgactgc atcgtgcctg tagccatcag caccagtaac
     7381 ttttaaccac cagacaccat tatccgtcat aaattcaagg gcttcataag agccgacaaa
     7441 atcaaacgaa ggc
//
LOCUS       DASWBY010000496         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_507978, whole genome shotgun sequence.
ACCESSION   DASWBY010000496 DASWBY010000000
VERSION     DASWBY010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507978"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..777
                     /locus_tag="VF870_08905"
     CDS             10..777
                     /locus_tag="VF870_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEX9252348.1"
                     /translation="MFFSVLNYFVKMVAPGGLHEIIYSIIQTPLTKMSQSLFSVLVLS
                     LLSQSLWAMGIHGPNTIAAIRDTMFSEAGNANLLHFAANGTTWGAPYPITYSGLVTAF
                     SEYGGSGGTLGLIIAMLIFSKNKESKSIAKLSLAPGLFNINEMVIFGLPIVLNPIYII
                     PFILTPLVTTMIGYTAVMILKIMPPVTIGIPWTTPGPLMPFLGTGGNITALFVGFICL
                     AVSVLIYSPFVIAANKAAVLEEEAENEISSQEVAESI"
     gene            843..1187
                     /locus_tag="VF870_08910"
     CDS             843..1187
                     /locus_tag="VF870_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEX9252349.1"
                     /translation="MDGMELIAFNIISNVGMAKSLAVEALRAAREDNYDEADEKIAEA
                     SEYLVNGHHAHASLIQREDAGDKVEFSLIIMHAEDQMMAAETTKLLIEEMIGMFKQLK
                     DKEVYTQEQKVQ"
     gene            1376..>2174
                     /locus_tag="VF870_08915"
     CDS             1376..>2174
                     /locus_tag="VF870_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9252350.1"
                     /translation="MNSTEIAKLAGVSRSTVSRVINNYSNVPEETREKVLRVIKEYDY
                     VPHASARMLAGSKNKVIGLFIVDLVNEEGGLKNRITKSPYYLEFTSSVIETASEMDYL
                     VLVHIIHTVAGYEKIKECFYNKMISGGIFIGQNDDDESIKKIIDRGYRVVLVDQSIRP
                     DDATYNQCTIVNADNYGGAYSATKYLTDMKHKQIAHITGGTAKFSSKDRIRGYKKALK
                     DAGIKVNKDLIINSEFVENAGYEATKKLFNGKSKFSAIFASNDKIAFG"
BASE COUNT          737 a          346 c          473 g          618 t
ORIGIN      
        1 atcacgacga tcttcttctc cgtactaaac tactttgtca agatggttgc accgggtgga
       61 ttgcatgaaa taatttatag tataatccaa acgccattaa ctaaaatgag tcaaagccta
      121 ttctcggtgc ttgttttatc attgttatct caatcgctct gggctatggg aatacatgga
      181 ccaaacacta tagcagccat aagagatacc atgttctctg aagcgggtaa tgcaaatctt
      241 ttacactttg ctgcaaatgg tactacatgg ggagctcctt atccgataac ttacagtggt
      301 ttagttactg ctttctctga atatggcggt tcaggaggaa cattaggtct aattattgct
      361 atgttgattt tctcaaaaaa taaagaatcg aagagtattg caaaactttc tttggcacca
      421 ggattattca atatcaatga aatggtcata tttgggttac cgattgtttt gaatccgatt
      481 tatatcattc catttatttt aacaccgttg gttactacta tgattggcta taccgcagtt
      541 atgattctta agattatgcc gccggtaaca attggtattc cgtggacaac tccagggcca
      601 ttaatgcctt tcttaggtac aggcggaaat atcactgcat tgtttgtcgg atttatctgt
      661 cttgcagtga gtgtattgat ttactctcct tttgttattg cagccaataa agccgcagta
      721 cttgaagagg aagcagaaaa tgaaatatcg tcacaagaag tagcagaatc gatctagacg
      781 gaatagctgt tgatgcggta ttcaaaagat aaggtgggcg attcagaata ggagatttac
      841 atatggatgg aatggaattg attgctttta atataatcag taatgttgga atggccaaat
      901 cacttgctgt tgaagctttg agagctgcca gggaagacaa ttacgatgaa gcagatgaaa
      961 aaatcgctga agcatcggaa tatcttgtga acggacatca cgcacatgct tccttgatac
     1021 aaagagaaga tgctggagac aaggttgaat tctcgctgat aatcatgcac gcagaagatc
     1081 aaatgatggc ggctgagaca acaaaattac tgattgaaga aatgatagga atgttcaagc
     1141 agttaaagga taaagaagtt tacacgcaag aacaaaaagt acagtgacta taattgttgt
     1201 ttaactcatt cacagaaaat gatgttgagg acaagataac ttaaatcgtt tagggcaccc
     1261 gggggataat atactcctgg gtgctctatt tttcgccaaa aagattgaaa aaaatgggta
     1321 aataatatag tgttgcatta tgaacacgag tttatgaatg agaggggatg cgtttatgaa
     1381 tagtactgaa attgcaaaac ttgcaggtgt atcccgcagt actgtatcca gagtaatcaa
     1441 taattattca aatgtcccgg aagaaacaag agaaaaagtt ttaagggtaa ttaaagaata
     1501 tgattatgtg ccacatgctt cagcaaggat gctcgcggga agcaaaaaca aggtgattgg
     1561 actttttatt gtagatctgg tcaatgaaga aggagggctg aagaatagga tcaccaaaag
     1621 tccgtattat ttggagttta ctagttccgt gatagaaaca gcaagtgaaa tggattactt
     1681 ggtccttgtt catatcatcc acactgttgc aggttatgaa aaaatcaaag agtgtttcta
     1741 caataagatg atttctggag ggatttttat aggccagaat gatgatgacg aatcgataaa
     1801 gaaaataatc gacagaggct atagagttgt attggttgac cagtcaatca gacctgatga
     1861 tgccacatac aatcagtgca cgatagtgaa tgctgacaat tacggtggtg cctatagtgc
     1921 aacaaaatat cttacagata tgaaacataa acaaatagca catattacag gtggtacggc
     1981 taaattctca tccaaagaca gaatcagagg atacaaaaaa gcattaaaag atgcaggaat
     2041 taaagtaaac aaggacttga tcataaacag tgaatttgtg gaaaatgccg gatatgaggc
     2101 tactaaaaaa ctattcaacg gaaaaagcaa attttcggcc atttttgcca gtaatgacaa
     2161 aatagcattt ggtg
//
LOCUS       DASWBY010000497         4417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_356505, whole genome shotgun sequence.
ACCESSION   DASWBY010000497 DASWBY010000000
VERSION     DASWBY010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356505"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..789
                     /locus_tag="VF870_08920"
     CDS             349..789
                     /locus_tag="VF870_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252351.1"
                     /translation="MKLIKIKGHYSDLGKIEMENNIIMNWNDLTDEEPMLIPFGSTIE
                     FSIAFEEHDFLNGKEGIVWASYDMRQIEIIKNALFAQNINAELKPRSLGKYKIFLLKI
                     NNNKDISDAVDFIWRSKTGLRLKPDWNYPEGTTNKSFEQWLSEH"
     gene            834..1370
                     /locus_tag="VF870_08925"
     CDS             834..1370
                     /locus_tag="VF870_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252352.1"
                     /translation="MKYLKPILFLIVVNICLTTSVIAQSEQGYILGMKNFEKAFPSET
                     NPWDDFDNLYMDTHYDGNIIKKGVKVNSDKVDGQEMLFEFDHFTVELVMNSNSALVNK
                     GTNNEYVVDANTKTIQLDNHRSLVSWLEDDNTYVTMYADFLNGQTSAFLYKDDGSVTP
                     LSGSIKIKKYMFEKSTTN"
     gene            1390..1824
                     /locus_tag="VF870_08930"
     CDS             1390..1824
                     /locus_tag="VF870_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252353.1"
                     /translation="MKTSVIFSFLLILLFAGANQMLAQSRDGNVFIVTTFERAFPENG
                     SANELDSLQQIMMDASYKSNPYVISYKTMSHWWGHNNRDFVQIIEVKSWDDISKAYDK
                     SEELIMKAMPNKADREKFSKAYDKYFTGKHSDEIYREVKFNK"
     gene            1853..2314
                     /locus_tag="VF870_08935"
     CDS             1853..2314
                     /locus_tag="VF870_08935"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9252354.1"
                     /translation="MKQLIGLSTFILIMIITFNTQAQQWTPEQKEVWAGVQKYWEINN
                     SDPQAYFKYFDDSYLGWSYDNETPGTKENAVKSSKYFSTKGKQQFNTITPARIWVNGD
                     FAFVHYYYTQVSESNDGKPTTEKGRWTDILMKKNGTWMIVGDHGGEIKKND"
     gene            2650..3648
                     /locus_tag="VF870_08940"
     CDS             2650..3648
                     /locus_tag="VF870_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252355.1"
                     /translation="MTIRALAVSGTNLFAGTAGGGVFISSDNGTNWSAVNNGLTGTTV
                     YGFAVLGTNLFVSMVGHGVFLSTNNGANWSQVNNGLTDNYVYALAVSGLNLFAGTVSS
                     GVFISTDNGANWTEANNGLTDTGISTFAVSGTNIFVATNSGVFRSSNNGTNWSEANSG
                     LPTERVETFTVSGTNIFAGTGQSGVYLSTDSGTNWTAINDGLTELTINALAILGSNLF
                     AGTYGSGVWRRPLSEVTSIEELTTNIPSSFTLEQNYPNPFNPSTTINFSIPNKSNVSL
                     KIFDLLGSQVAELLNGEIEAGSYNVQYNAANLPSGIYFYKIQSDNFSETKKMVLLK"
     gene            3756..4238
                     /locus_tag="VF870_08945"
     CDS             3756..4238
                     /locus_tag="VF870_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9252356.1"
                     /translation="MKKIINTFILLISFVLVIPSFAINNKPAKESNVEIIQNLYAAFA
                     IGDVPAVLQNFDPKIVWNEAENFPYADRNPYVGPQAVLEGVFARIGAEWEYWNLTDQT
                     YYEANSGEIIVTGRYNAKNKLTGKEISVQFVHMWTLNEGLVTKFQQYADTYQAVEAMK
                     "
BASE COUNT         1520 a          744 c          827 g         1326 t
ORIGIN      
        1 ttctatgata gatggagcgg ataaatggct gattaaatca cgtcaatgaa taatgttaca
       61 attcaattgc aagcctaggt agaaatatta atatctgctt tcttgtaatg ccaattctat
      121 taattcttac taaattcctc cgcttcacct aagtccccct ttctatatct tattataaat
      181 aagatagatc catccccacc agtccaaatg tcaaataatg ttatataaaa cagaaaagtc
      241 caaacatatg cgagaatagt gcaagatatc agctaatcaa tattctactt ttgtaataag
      301 caatttccaa acaattaaat taaaaaaaac taaaatctct ggatttctgt gaaacttata
      361 aaaataaaag gtcattattc agatttaggg aaaattgaaa tggaaaataa tataattatg
      421 aactggaacg atttaactga tgaagaacct atgctgattc cttttggaag tacgattgaa
      481 ttttctattg cttttgagga acatgatttt cttaatggta aagaaggaat tgtttgggca
      541 agctatgata tgcggcaaat tgaaataatt aaaaatgctc tgtttgcaca aaatataaat
      601 gctgagctaa aacccaggag tcttggtaaa tataaaattt ttcttttgaa gataaacaac
      661 aataaagata tatctgacgc agttgatttt atctggagaa gtaaaaccgg actgcgtctt
      721 aaacctgatt ggaattatcc tgagggtact accaacaaga gttttgaaca atggttaagt
      781 gaacattaaa taattatgaa tttaacttat taaataattt caaggagaat gaaatgaaat
      841 acttaaaacc gattctgttt ttgatagtag ttaacatttg tttaaccact tctgtaattg
      901 cgcaaagtga acagggatat atacttggaa tgaaaaattt cgaaaaagct ttcccttctg
      961 aaactaatcc ctgggatgat tttgataatc tttatatgga tacccattat gatggtaaca
     1021 ttataaagaa aggagttaaa gttaattcgg ataaggttga tgggcaggaa atgttatttg
     1081 aatttgatca tttcacagtt gagttggtaa tgaattctaa ttctgctctt gtgaataaag
     1141 gaactaataa tgaatatgtc gtagatgcaa acactaaaac tatccaactc gataatcata
     1201 gatcattagt atcctggctt gaagatgata atacatatgt aacgatgtat gcagattttc
     1261 tcaatggtca aacttcagca ttcttataca aagatgatgg gagcgttact ccattatctg
     1321 gttcaattaa aattaagaaa tatatgttcg aaaaaagtac gactaactaa ccaaaaagga
     1381 gcaagtgcta tgaaaacttc agtaatattt tcttttcttt taattctttt atttgcaggc
     1441 gcaaaccaaa tgcttgcaca atccagagac gggaatgtat ttatagtaac aacatttgaa
     1501 agagcgtttc cagaaaacgg ctctgcgaat gaattagatt ctctgcaaca gattatgatg
     1561 gatgcttcat ataagagtaa tccatacgtt ataagttata aaaccatgtc tcattggtgg
     1621 ggacacaata atcgtgattt tgttcaaatt atagaagtga aaagctggga tgatatctca
     1681 aaagcttatg ataaatcaga agaactgatt atgaaggcaa tgcctaacaa agccgacaga
     1741 gaaaaattca gtaaagcata cgataagtat ttcaccggaa aacactctga tgaaatttat
     1801 cgtgaagtaa agtttaataa ataatatata ataccaaaaa ggagacaata ctttgaaaca
     1861 attaattggc ctttcaactt ttatcttaat aatgattatt acattcaata cgcaagcaca
     1921 gcaatggaca ccagaacaaa aagaagtttg ggcaggcgtt cagaaatact gggaaataaa
     1981 taacagtgac ccacaggctt actttaaata ctttgatgat tcatatcttg gctggtctta
     2041 cgataacgaa actcctggta caaaagagaa tgctgtaaaa tcatctaagt acttttccac
     2101 aaaaggaaaa cagcagttca atactattac acctgccaga atatgggtca acggggactt
     2161 tgctttcgta cactattact acactcaggt ctccgaaagt aatgatggta aaccaactac
     2221 agaaaaaggt cgctggactg atatcttaat gaagaagaat ggcacctgga tgattgttgg
     2281 tgatcacggc ggcgaaatta agaagaatga ctagttgtta agtaaaaatt atttaggaaa
     2341 acaggcagag tattatctgc ctgttttaat atcatatcaa tcataaacaa aatgaaagga
     2401 tcgggacaat cgatgaaaag cttctacaag tttttcggga taattttatt ttttgggtta
     2461 atgatttcaa tatcattttc gacccaactg cgggcacaat ggattcaaac cagtggaccc
     2521 agcgataaca atatcttttg tcttactgcc tccgtcacaa atatttttac tggaactcag
     2581 agcggtggtg tttttctttc ttcggatatt ggggaaagtt ggattgcagt caatactaac
     2641 ctgactaatt tgacgatccg ggcgcttgcc gtttccggca cgaatctttt tgcaggaacg
     2701 gcgggtggag gtgtctttat ttcgtccgat aacgggacaa actggtctgc agtaaataat
     2761 ggcttaacag gtaccactgt ttatggtttt gccgtcttag gcacgaatct ctttgtctca
     2821 atggtgggcc acggcgtttt tctttccacc aataacggtg caaactggtc tcaagttaat
     2881 aacggcttga cagacaatta tgtttatgca cttgcagtct ccggtttaaa tctctttgcc
     2941 ggcacagtga gcagcggcgt ttttatttcc accgacaacg gtgcaaactg gacggaagcg
     3001 aataatggac taacagatac cggcatcagt acatttgccg tttccggcac gaacattttt
     3061 gttgctacca atagcggtgt attccggtca tccaacaatg gaacaaactg gtctgaagca
     3121 aattccggtt tgccaacgga gcgtgttgaa accttcacag tttccggcac taatattttt
     3181 gccgggaccg gtcaaagtgg cgtctatctt tcaactgata gcggaacaaa ctggacagcc
     3241 atcaatgacg gtttgacaga acttacaatt aatgcactgg cgattttagg ttcaaatctg
     3301 tttgccggca cctatgggtc aggagtctgg cgcagacctt tgtcagaggt tacatctata
     3361 gaagaactca caacaaatat tccatccagc ttcacactgg aacagaacta tcctaatccc
     3421 tttaatccat ctacaacaat aaatttttcg attcctaata agtcaaacgt aagcttaaaa
     3481 atatttgatt tattgggtag tcaagttgca gaattattaa atggtgagat agaggcagga
     3541 agttataatg ttcaatataa tgcggctaac cttcccagcg gaatttattt ctacaaaatt
     3601 cagtcggata attttagcga aactaaaaag atggtactac ttaaataaaa agcatttaat
     3661 aaaacaggca gtgaaatatc tgcctgttca tttttaatca ataattattg agtatacaat
     3721 aattaattat ttgggaacat tattaagagg agacaatgaa aaaaataatt aacactttca
     3781 tcctgctaat atcttttgtc ctagtaatcc cctcttttgc tataaacaac aagcctgcaa
     3841 aagaaagcaa tgttgaaatc attcagaatt tgtatgctgc ttttgctata ggtgatgtac
     3901 cggccgtact gcaaaatttt gatccaaaga ttgtatggaa tgaagcagag aattttcctt
     3961 atgcggatcg gaatccttac gttggtcccc aggcagtact cgaaggtgtt tttgccagga
     4021 ttggtgcaga atgggaatac tggaacctaa ctgatcaaac ttattacgaa gctaattcag
     4081 gtgagataat agttaccgga cgttacaacg caaaaaataa attaacaggc aaggaaataa
     4141 gtgttcagtt tgtgcatatg tggacactta acgagggttt ggttacaaag tttcagcaat
     4201 atgctgatac ttatcaggcc gttgaggcga tgaaataaga acaataatat cattttcaat
     4261 aaactaataa taaggaacta gtactatgaa attaacaaaa ttatttactg caattattct
     4321 tattgcaatt ctttccctca gtggaaaaaa cttcgcccaa caatggtcac ctgaacaaaa
     4381 agacgtatgg tcaggagtac aaaagtactg ggacgtc
//
LOCUS       DASWBY010000498         3989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_282038, whole genome shotgun sequence.
ACCESSION   DASWBY010000498 DASWBY010000000
VERSION     DASWBY010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282038"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1682
                     /locus_tag="VF870_08950"
     CDS             <1..1682
                     /locus_tag="VF870_08950"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX9252357.1"
                     /translation="LTNNILMMYIFWELVGLSSYLLIGFWFEKKSAADAGKKAFLVNR
                     IGDIGMFAGILILFLTYNTFSFDTIFQSIGAGKLPFNSDFWLTVTGILLFMGAIGKSA
                     QFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLTIRIFGLLTADAMMFIAVIGMLSA
                     FIPATIALTQNDIKKVLAYSTVSQLGYMIMALGVGAYKFAFFHLVTHAFFKACLFLGS
                     GSVIHAMHHEQDIRNMGGLRKKLPLTYATFLISSLAISGIPLTSGFLSKDGILASTFA
                     FGSLTGYWIFAVVGFLVALLTAFYMFRLIIITFHGEPRDHHKFEHAHESPFVMAMPLA
                     VLAMLSIFIFYTPNPFSADQGWFFNKWINSPELHAPNSTRFDFMQSEVIAGTEQVAEQ
                     HSAHGEITYSEKYTEAMHTAHYPAMFLSLFVAVLGILTAFVFYQWKKIDVEKLAVSIK
                     PLYNFSLNKWYFDELYHKTFVAGLLGLSKIIYWFDATIVDGLVNGSAEVTRRVAFFTG
                     GFDKYVVDGLVNLMAYISGFIGLIFRRVQTGKVQTYLVLVIFSIIILLFLF"
     gene            1714..3318
                     /locus_tag="VF870_08955"
     CDS             1714..3318
                     /locus_tag="VF870_08955"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX9252358.1"
                     /translation="MNNFPILTLLTFLPILGMVIVLLLPKKQELSIKVTTLLVSAIQI
                     VLSIILLMNFNYAAGGINDANSFQFIEKFRWIDIPGFAWIGQIKIDYFLGVDGLSTPL
                     VLLTAIVTFIAAISSWTIDKSVKGYFAMFLLLNTGMMGVFVSLDFFLFYIFWELMLLP
                     MYFLIGIWGGPRREYAAIKFFLYTLLGSIFILLVMIGLYFSSMETLADGTRVFTFNLL
                     ELMNHANYTATGILSPLNPNNLRFIAYIGLFIGFAIKIPMFPFHTWLPDAHVEAPTAI
                     SVILAGVLLKMGTYGILRVSYPIFPELTYQLSWYIALFGMINIIYGALAAMAQKDFKK
                     LIAYSSVSHMGFVMLGMSALNTMGISGAILQMFNHGTITAMLFLIVGVIYDRAHTRDL
                     DSFGGLGKQMPIYTGFVTIAFFAAIGLPGLSGFISEALVFLGGFGNGTTRILAVISTL
                     GILLGAAYMLWTLQRVYLGTLNEKWKDLTDLTTREYFMLVPLTIIVIYLGVNPSPMLN
                     LMNASANAMVKFISDAQVIYSSVNAF"
     gene            3339..>3989
                     /locus_tag="VF870_08960"
     CDS             3339..>3989
                     /locus_tag="VF870_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9252359.1"
                     /translation="MILPNLSADLQAIFPEISLVLTLVAIVVFDLIFNKSKKFLPLIS
                     LIGLVVTFIFVVLQFGDPKSAFSVSGKLSFLSLDNFSSFFKILILITTAFIVLFSMSS
                     QEVNSCPDRHGEYYALIFGMVIGMFFMVSANDLILIYLSLELLSLSSYVLAGFVKTSI
                     RNSEASLKYIIYGSASSGIMLFGISILYGITGSTNLHEINSLLQFPAASQLTLLLAI"
BASE COUNT         1149 a          676 c          713 g         1451 t
ORIGIN      
        1 tactaaccaa taatattttg atgatgtaca ttttctggga gctggttgga ttatcatctt
       61 accttttgat tggattctgg tttgagaaaa aatccgctgc cgatgcaggt aagaaagcat
      121 tcttagtaaa taggattggt gatatcggaa tgtttgcagg tattttgatt ttatttctta
      181 cctacaacac attttctttt gatacaattt ttcagtcaat tggcgcaggt aaacttcctt
      241 tcaattctga tttctggtta actgtaaccg gcattttatt atttatgggc gcaattggaa
      301 aatcagctca gttccctctt catgtttggt taccggatgc aatggaaggc ccgactccgg
      361 ttagcgcatt gattcatgct gcaacaatgg ttgccgcagg tgtttatctt acaattagaa
      421 tttttggatt gttgactgct gatgcaatga tgtttattgc agtaatcgga atgttatcag
      481 catttattcc tgcaacaata gcacttacac aaaatgatat caaaaaggta ttagcatact
      541 caactgtatc tcaattgggc tatatgatta tggcacttgg agttggtgct tacaaatttg
      601 ctttcttcca tcttgtaaca catgctttct ttaaagcttg tttgtttctt ggttccggtt
      661 cagtgattca cgcaatgcat cacgaacagg atattagaaa catgggtgga ttaagaaaaa
      721 aacttccgct tacttatgca acatttttaa tttcatcact tgcaatttct ggtattccac
      781 taacatctgg atttttaagt aaggatggaa tacttgcaag tacatttgca tttggttcac
      841 tcacaggtta ttggattttt gctgtagtcg gatttttagt tgctctgtta acagcattct
      901 atatgttcag attaattatt ataacatttc atggtgaacc aagagatcat cacaaatttg
      961 aacacgcaca cgaatctcct tttgttatgg caatgccgtt agcagtactt gcaatgttat
     1021 caatatttat tttttatact ccaaatccgt ttagtgcaga tcaaggatgg ttttttaata
     1081 agtggataaa tagtccggaa ttacatgctc caaattctac tcgttttgat tttatgcaat
     1141 cagaagttat tgcaggaaca gaacaggttg cagaacagca ttcagctcac ggagagatta
     1201 cttattctga aaaatatact gaagcaatgc atacagcaca ttatcctgca atgtttcttt
     1261 cattatttgt ggcggtttta ggaattttga ctgcattcgt tttttatcaa tggaaaaaaa
     1321 ttgatgttga aaaactagct gtctcaatca aaccacttta taatttctcg cttaacaaat
     1381 ggtactttga tgaactgtat cacaaaacgt ttgttgcggg actgctcggc ttaagtaaaa
     1441 tcatttactg gtttgatgca acaattgttg atggacttgt aaacggctct gctgaagtaa
     1501 cacgcagagt agctttcttt acaggtggat ttgataaata cgtagttgat ggacttgtaa
     1561 acttaatggc atacatcagc ggatttatcg gtttgatttt ccgcagagta caaacaggaa
     1621 aagttcagac ttaccttgta ctcgtaatat tttcgataat tattttactc tttttattct
     1681 aagtataaag tttgaaaaac aggtaacaca accatgaata attttcctat acttacatta
     1741 ttaacgttcc tcccaatatt aggaatggta atcgttctgc tccttcccaa gaagcaagag
     1801 ctttcaataa aggttaccac actccttgta tcagcaatac aaattgttct atcaataatt
     1861 ttattgatga acttcaatta tgctgcgggt ggaataaacg atgcaaactc atttcaattc
     1921 atagaaaaat tcagatggat tgatattccg ggttttgcct ggattggcca aattaaaata
     1981 gattattttc ttggtgttga tgggttaagc actccgcttg ttcttctaac agcaattgtt
     2041 acttttattg cagctatatc ctcctggact atcgataaat cggtaaaggg atattttgca
     2101 atgttcctgc ttttgaatac cggaatgatg ggcgtatttg tatcacttga tttcttcctg
     2161 ttctacattt tctgggaatt gatgttactt ccgatgtact ttttaattgg tatctggggc
     2221 ggtccaagaa gagaatatgc agcaataaaa tttttcctct acactttgct tggaagtatt
     2281 ttcattttat tagttatgat aggattgtat ttcagttcta tggaaactct tgcagatgga
     2341 acgcgagtgt ttacatttaa cttattagaa ttgatgaatc atgccaatta tacggcaaca
     2401 ggaattttat cgccgttaaa tccaaacaat ttaagattca tagcctacat tggtttgttt
     2461 atcggatttg caattaaaat accaatgttc cctttccaca cctggcttcc agatgcgcac
     2521 gtagaagccc caacagcgat tagcgttatt cttgccggtg ttctgttgaa gatgggaact
     2581 tacggaatac tcagagtttc atatccaata tttccggaat taacatatca gttaagctgg
     2641 tacattgcac tgtttggaat gataaatatt atctacggtg cacttgctgc tatggctcaa
     2701 aaagacttta aaaaactaat tgcttactcc tctgtttctc atatgggttt tgtaatgctt
     2761 ggtatgtcag cactaaacac aatggggatt tcaggggcaa tacttcaaat gtttaatcac
     2821 ggtacaatca cagcaatgct cttccttata gttggagtta tttacgaccg tgctcataca
     2881 agagatttag attcgtttgg tggattagga aaacagatgc ctatttatac aggatttgtg
     2941 accattgcat tctttgctgc aattggtcta ccgggtttaa gtggatttat ttctgaagcc
     3001 ctggttttct taggcgggtt tggaaacgga acaacaagaa tattagctgt tatttctacg
     3061 cttggtattc ttttaggagc agcttatatg ctctggacat tgcaacgagt ctatcttgga
     3121 acactaaatg aaaaatggaa agatttaaca gatcttacaa cacgtgagta ctttatgctc
     3181 gttccattaa ctattatcgt tatttatctt ggagttaatc catcaccaat gcttaacctt
     3241 atgaatgctt ctgcaaatgc tatggttaaa tttattagtg atgcacaagt aatatatagt
     3301 tcagtaaatg cgttttaatt ttatttaaag tgataacatt gattttacca aacttatctg
     3361 ctgatcttca agcaatattt cctgagattt ctctggtgtt aacgcttgtt gctattgttg
     3421 tctttgattt gatattcaat aaaagtaaaa aatttcttcc attaatttct ttaatcggat
     3481 tagttgttac ttttattttt gtagttcttc aatttggtga tcccaaatca gcattctcag
     3541 tttcaggaaa attatcattt ctgtctttag ataatttttc atccttcttt aaaatattaa
     3601 tactcattac aactgctttt atagttttat tttcaatgtc atctcaggag gtaaattcct
     3661 gtcctgatag acatggtgaa tattatgctc taatatttgg aatggtaatc gggatgttct
     3721 ttatggtttc tgcaaatgat ttaattctaa tttatctttc attagaatta ctttcattat
     3781 catcatatgt tctggcagga tttgtaaaaa cttcaattag aaatagcgaa gcttcactta
     3841 aatatatcat ttacggttca gcatcatctg gaataatgtt gtttggaata tcaatcctat
     3901 atggaattac aggcagcaca aatctccacg aaataaattc attgcttcaa ttccctgctg
     3961 ccagccagtt gaccttatta ttggcaata
//
LOCUS       DASWBY010000499         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_356601, whole genome shotgun sequence.
ACCESSION   DASWBY010000499 DASWBY010000000
VERSION     DASWBY010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356601"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VF870_08965"
     CDS             complement(<1..314)
                     /locus_tag="VF870_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9252360.1"
                     /translation="MSPEILTAFFVGLFGSFHCIGMCGPIAIALPVPNSNNLSFVTGR
                     LLYNLGRVTTYSFLGAVFGLLGSRLVISGFQQSVSIVLGIAIVIAVLIPPKYKVKITQ
                     HKL"
     gene            complement(474..926)
                     /locus_tag="VF870_08970"
     CDS             complement(474..926)
                     /locus_tag="VF870_08970"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX9252361.1"
                     /translation="MKKNKFNWGTGILITIILFMIITISTVVFLMNQDVDLVASDYYD
                     KGIHHQEQIDRMNRTNKMGNEVSITPENGFIRLMLPKYFAQKSLSGTIQFYRPSDSKK
                     DFAVAVALDTSAQQLISTKSMAKGYWKVKLNWTQDAVEYYKESSFVIN"
     gene            complement(913..2352)
                     /gene="ccoG"
                     /locus_tag="VF870_08975"
     CDS             complement(913..2352)
                     /gene="ccoG"
                     /locus_tag="VF870_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX9252362.1"
                     /translation="MKTDEMLIAEDNQEYRNQLATVTDDGKRKWVYPKKPSGKFYNAR
                     TILSFFLLAFLIIVPFIKVNGHQFLLFDFLNRNFILFGIPFGPHDFHLFVLAMIAIIV
                     FIILFTVVFGRIFCGWACPQTVFLEMVFRKIEYWIEGDAKDQRKLDAEGWNGKKLFKK
                     SLKNIIFFIISFFIAHIFLSYIISLDKVISIITQPPSAHLSGFVAIIAFSLIFYWVFA
                     FFREQACTIVCPYGRLQGVLLDQDSIVIAYDYKRGEPRGKIKKNENQDNKGDCIDCNL
                     CVDVCPTGIDIRNGIQLECVNCTACIDACDAVMEKINKPKKLIRYDSLNGIENKTKFK
                     VTPRMILYSAILFVLIGILGYLLSVRTDYSVNVLRTPGMLFQEQPNGKVSNVYDLNIV
                     NKTFHETPVQIKLENPADGELKLVGKEIVLKSQEIVETKFLVLLNKDGIKKMNTPIEI
                     GVYEGSKLIKKVKTSFLGPVKRDEQDEKK"
     gene            complement(2382..>2854)
                     /locus_tag="VF870_08980"
     CDS             complement(2382..>2854)
                     /locus_tag="VF870_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9252363.1"
                     /translation="YMLDYHVFNSSPLQAEEYQIQVKQAELERAALINSGAFLNEESV
                     TLLKDPAALDAGKQIFATNCVACHAADGGGLVGPNLTDDYWIHGGGIKNIFKTIKYGV
                     TAKGMIAWQSQLNPKQIQDVASYVISLHGTKPANPKQPEGTIWKEADSTAVASN"
BASE COUNT          923 a          547 c          427 g          957 t
ORIGIN      
        1 agtttatgtt gagttatttt aactttatac tttggcggga ttaagactgc aattacaatt
       61 gcaattccta gtacgataga aacgctttgt tgaaaaccag aaattacaag tcttgaaccc
      121 agcaacccaa aaactgcacc tagaaaggag taagttgtta ccctaccaag attataaagc
      181 aaccgacctg taacaaatga taaattatta gagtttggaa caggaagtgc gattgcaatt
      241 ggtccgcaca ttccaataca atgaaagctt ccgaataatc caacaaagaa tgctgttaat
      301 atttctggtg acatttttaa tctgtaaaaa aataaagaat ctaattaaaa gtaaaagtca
      361 tttcgaagga gtcgtcgaca gagaaatcta aattactaga ggggaaagat ttctcccttc
      421 ggttgaaatg actattactc ttttcattca attcaaaaat cattcataat ggtttagtta
      481 attacgaaag aactttcttt ataatactca accgcatctt gagtccagtt aagtttaact
      541 ttccagtatc ctttagccat acttttagtt gagattaatt gctgagctga tgtgtctaaa
      601 gctactgcaa ctgcaaaatc ctttttagaa tcggaaggac gatagaattg gattgtaccg
      661 cttaaacttt tttgagcaaa atatttggga agcattaatc ttataaaacc attttctggt
      721 gttatgctta cttcatttcc cattttattt gtcctgttca ttctatctat ctgctcttga
      781 tgatgaatgc ctttatcgta ataatcactt gcaaccaaat caacatcctg attcattaaa
      841 aaaacaaccg tactaattgt gattatcatg aaaaggatta tggtaatcaa aatccctgtt
      901 ccccaattaa atttattttt tttcatcttg ctcatccctt tttaccgggc ctaaaaatga
      961 tgtctttact ttttttatca atttgcttcc ttcgtacact cctatttcaa ttggagtgtt
     1021 catcttttta attccatcct tattcaacag cactaaaaat tttgtttcaa caatctcttg
     1081 agatttcaga acaatttctt tcccaactaa tttaagttca ccatcagcag gattttcaag
     1141 ttttatttgt actggtgttt catgaaaagt tttgttaaca atattaagat cgtatacgtt
     1201 acttactttt ccattcggct gttcctgaaa taacattcca ggcgttcgta aaacattaac
     1261 tgaataatcg gttctaactg aaagtaaata tccaagtatt cctatcaaca caaaaagaat
     1321 agctgaatat aatatcattc ttggagtaac tttaaattta gttttattct ctatcccatt
     1381 gagggaatca tatcggataa gttttttagg tttattaatt ttttccatca cagcatcgca
     1441 agcatcaatg cacgcggtac aattgacaca ttccaattgg attccgtttc taatatcaat
     1501 tcctgttgga cacacatcaa cacataagtt gcaatctata caatcacctt tattatcctg
     1561 attttcattt ttcttgattt taccgcgcgg ttcacctctc ttataatcat aagcaataac
     1621 aattgaatcc tgatcaagca aaacgccttg caaccttcca taaggacaaa ctattgtaca
     1681 ggcttgttca cggaaaaatg caaaaaccca atagaagatt aatgagaagg ctatgattgc
     1741 aacaaatccg ctaagatgag cactcggcgg ttgagttatt atcgaaatta ctttatccaa
     1801 actaattatg taggataaaa agatatgtgc tataaaaaat gaaatgatga aaaagataat
     1861 attcttcaga ctctttttaa aaagtttttt gccattccac ccttcagcat caagtttgcg
     1921 ctgatccttt gcatcgcctt cgatccagta ttcaatcttt ctaaaaacca tttccagaaa
     1981 aactgtttgc gggcaagccc atccgcaaaa tattcttcca aacacaacgg taaacagaat
     2041 tatgaaaacg ataattgcaa tcattgcaag aacaaagaga tgaaaatcgt gcgggccaaa
     2101 aggaatacca aacaggataa aatttctgtt cagaaaatca aacagaagaa actgatgacc
     2161 gtttactttt ataaaaggaa caattattaa aaatgccaga aggaaaaaac ttagaattgt
     2221 tcttgcgtta taaaatttac ccgaaggctt cttcgggtaa acccattttc tttttccatc
     2281 atctgttact gttgcaagtt gatttctata ctcttgatta tcttcagcaa ttagcatttc
     2341 atctgttttc ataacactct cataaaatta ttattgctgc attagtttga tgcaacagca
     2401 gttgaatcag cttccttcca aatagtgcct tcaggctgct ttggatttgc aggtttagtg
     2461 ccgtgcaaag aaattacata gctcgcaaca tcctgtattt gttttggatt taactggctt
     2521 tgccaggcaa tcatcccttt tgcagttact ccgtacttta ttgttttaaa tatattttta
     2581 attccgcctc catgaatcca atagtcatca gttagatttg gccctactaa tccgccgcca
     2641 tcagcagcat gacaagcaac acaattagtt gcaaagattt gcttgcctgc atcaagtgcc
     2701 gccggatctt ttaaaagagt aaccgattct tcatttaaaa atgcacctga attaattaat
     2761 gctgctcttt caagttcggc ctgtttaact tgtatttgat attcctcagc ctgtaatgga
     2821 cttgagttaa aaacatggta gtctaacata taaa
//
LOCUS       DASWBY010000500         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_698647, whole genome shotgun sequence.
ACCESSION   DASWBY010000500 DASWBY010000000
VERSION     DASWBY010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698647"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VF870_08985"
     CDS             <1..940
                     /locus_tag="VF870_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252364.1"
                     /translation="DQDEQNIYKEAMPAFINYLYNEKDDYIPLPPDEYHVAEQLFSSS
                     MAITKDEDLQHKIRESASFYLSSHYEKVMNAGDWSARVNALYYIEDFRMTSLQPVLAK
                     RLMAVKEQDDEKQQLLRTLAALGDLSVLSYLENEPSAKESVYLSVFKRFPEDVIPQTV
                     SYVRQEGSLKANISLIMYLGLAKRLSYLSFVEENLSHQNSEMRIQALKALFQMNFISN
                     VNLIYPFFYSEIWEERMFAAKITGVLSLTQFEKELTEFLGDSVWWVRYYAAEALLLSF
                     GESKLIELSQNHPDQYARNMAAQWLRLERQVSVAHD"
     gene            954..>1758
                     /locus_tag="VF870_08990"
     CDS             954..>1758
                     /locus_tag="VF870_08990"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9252365.1"
                     /translation="MFGFLSIIVVFYMTFTALSYIILFLLSFRKVSREGKLDKKEMLE
                     DLANSETTIPVSIIVPAYNEEIGIISTVRSLLTLQYPKFEIIVVDDGSKDQTLTKLIE
                     AYGLEVIDYAIRKHVDTKPIHVVYKSTIFPNLVVVSKENGGKADALNSGINLSNFPYF
                     CAIDGDSILEQDALLKAMKPVLAADGNIIAVGGSIRIANGTTISRSKVEVIELPNKPL
                     VIMQIVEYFRAFLIGRLGFSRFNQLLIVSGAFGVFRKDKVIRVSGYQEGT"
BASE COUNT          566 a          290 c          337 g          565 t
ORIGIN      
        1 agaccaagat gaacagaata tttataaaga agccatgccc gcattcataa actatcttta
       61 taatgagaaa gatgactata tcccattacc tcctgatgag tatcatgtgg ctgaacaact
      121 atttagctct agtatggcga ttacaaaaga tgaagatctg cagcacaaaa tccgcgaaag
      181 tgcatctttc tatttaagtt cacactatga aaaagtgatg aacgctggag attggtctgc
      241 tcgggtaaat gcactgtatt acatagaaga ttttcgcatg acttcattgc aaccagtact
      301 tgccaagaga ttaatggccg tcaaagagca ggatgacgaa aaacagcagt tgttgcggac
      361 tttagcagcc cttggtgact tatccgtatt atcatattta gaaaatgagc ccagtgcaaa
      421 agaatcagta tatctttctg ttttcaaacg ttttcccgaa gatgtaatcc cacaaacagt
      481 ttcatatgtt cgtcaggaag gaagtttaaa agctaatata tctctaataa tgtaccttgg
      541 actcgcaaaa agattatcct atttgtcttt tgttgaggaa aatctttctc atcaaaattc
      601 tgaaatgaga attcaagcat taaaagcttt atttcagatg aactttatat caaatgtcaa
      661 tttgatctat cccttttttt attcagaaat ttgggaagaa cgaatgttcg cagcaaaaat
      721 cactggtgta ttgtccctaa ctcaatttga aaaagaacta actgaatttc taggagattc
      781 tgtctggtgg gttcgatatt atgctgcgga agcgttattg ctttcttttg gtgaaagtaa
      841 acttattgaa ctctcacaaa atcaccctga ccaatatgca cgaaatatgg cagctcaatg
      901 gttacgtctt gaaaggcagg tttctgttgc acatgactaa tatttggaat tggatgttcg
      961 gatttttatc tataattgtt gtgttttata tgacttttac tgccttaagt tatatcatct
     1021 tatttttact atcttttcga aaagtttcaa gagaaggaaa acttgataaa aaagagatgt
     1081 tggaagattt agctaatagt gaaactacaa ttccagtgtc tatcatcgta cccgcttaca
     1141 atgaagaaat cgggattatt agtaccgttc gatcattatt aactttgcaa taccccaaat
     1201 ttgaaatcat tgtagtcgat gatggatcaa aggatcaaac acttaccaaa ttgattgaag
     1261 cttacggttt agaagtaatt gattatgcta ttcgaaaaca tgtagatacc aagccaattc
     1321 atgtggttta taagtctact atttttccaa acttagtagt ggtatcaaaa gaaaatggtg
     1381 gcaaagcaga tgccttaaat agtggaatta acttatccaa tttcccgtat ttttgtgcta
     1441 ttgacggtga ttcaatatta gagcaagatg cgctattaaa agccatgaaa cctgttctag
     1501 ctgctgatgg aaatataatt gcggttggtg gatccatacg aattgcgaac ggtacaacca
     1561 tttctaggag taaagtagaa gtcattgaat taccgaataa accattagtt attatgcaaa
     1621 ttgtagagta tttccgtgca tttcttattg gacgtcttgg attcagtcga tttaatcagc
     1681 tgttgattgt gtcaggagct tttggcgttt tcagaaaaga caaggttatc cgtgtgagtg
     1741 gataccaaga aggcactg
//
LOCUS       DASWBY010000501         5519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_585119, whole genome shotgun sequence.
ACCESSION   DASWBY010000501 DASWBY010000000
VERSION     DASWBY010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585119"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VF870_08995"
     CDS             complement(<1..582)
                     /locus_tag="VF870_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252366.1"
                     /translation="MMAKNKKRERKFLPVFIILAITADLAFLYFNKYNNQNLTLRDFN
                     IFYFGNIINLLFVLLIIIGLLVIIFKKSIVFNLKIFIPVFLINQFILVTLYFLKYIPL
                     PFKKFYYLGQTGDELFIGAVFFIYTLTFLIMIFLVWLSIFNFKKIILFRAFANSILSL
                     IIILLMVFLFIIGKESTIQGGLLKINKNNVGVVL"
     gene            complement(730..1389)
                     /locus_tag="VF870_09000"
     CDS             complement(730..1389)
                     /locus_tag="VF870_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX9252367.1"
                     /translation="MAIVKSKKADLMAKYKRVFEISLIAALALLIVAFKFFPNLQGKE
                     VVTEGPQELFQVEQIDQTKQENRPPPPPKPPIPIEAPSSDVLEDIEIGDTEIDINEQM
                     DAPPPPPKEDKKVVEEEPVYFVAVEEMPEPIGGISEIQKKIVYPEIAKRAGVEGKVYV
                     LAFVDESGTVTDAKIIKGIGAGCDEAALDAVKKTKFKPGKQRGKAVRVQVSIPVVFKL
                     Q"
     gene            complement(1483..1911)
                     /locus_tag="VF870_09005"
     CDS             complement(1483..1911)
                     /locus_tag="VF870_09005"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX9252368.1"
                     /translation="MYSFLQKYRFYIVNVPLVIYWIILLILTSLPSSMAINMDVSDKI
                     EHFGAYGLLGVFLYLNLFLQEKFALLKKYPATFTVIIASIYGLLDELHQLFVPGRSAE
                     LLDWLADFLGSLTAVLITRYLVKKIKQNEIEKSKLKAGTF"
     gene            complement(1904..3460)
                     /locus_tag="VF870_09010"
     CDS             complement(1904..3460)
                     /locus_tag="VF870_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX9252369.1"
                     /translation="MIPLYNTSQIRNLDSFAIKRLNAPGIVLMENAALGIYQSILEHF
                     DNLKSVGVVCGKGNNGGDGYAVARHFSNSGFDVKVISLGSEKDLSEDCKTNFNILKNL
                     SSQRKNLLLKSFKSIKDISWLKDCDVIIDAILGSGFTGELKEPYASIIKSLNKISTIK
                     CAIDVPTGLNADTSFGNIVFNSDLTITLGEFKKGLFIGMGYEFCGEVVLKEIGIGRDY
                     FDDIYPDTYLIEPEDVYSSLPKRGKTVHKYSAGKVLSIAGSFKYPGAASLTSQSALIS
                     GAGASILSIPESTKKLIHKNLLEVVVESYGNEKTKYFDNDGLDSLQQKIKWADVVAIG
                     PGLGREAETIIAVKRFVVERKFNFAVIDADALFALDKEFLNHCNLENCILTPHIGEFS
                     QLIGIEAKLIEKDILSYGKDFAEKHKCILVLKGASTIIFTPGGEAFINTTGNSGMAKF
                     GSGDVLTGLIVGLLSQSKNPEAAAIAGVYLHSLSADLLLTEKPISNYLASDTMNNYPA
                     AIKFLEASIV"
     gene            complement(3579..5006)
                     /locus_tag="VF870_09015"
     CDS             complement(3579..5006)
                     /locus_tag="VF870_09015"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX9252370.1"
                     /translation="MINNFQEFFSILPLVLIGTGIVISLLIEMYSKKSEFLLPWFSVL
                     LFLAAGLHSLYYVGDVSIVMQNMLATGGNVNVFYAIFTLGAAVVCLLSIDYIKKYGTY
                     FGEYYIILQSSVLGMMLMAGAKDLFVIFLGLELMSVSFYILAGMNRKKLNANEASLKY
                     FLLGAFATGFIVYGIALIYGSAQTTSIDFITLHFADLTKNLLFVAGVLLFLVGFSFKI
                     AAFPFHMWVPDVYEGSPTTVAALFSTSGKAAAFSAIIATLFALFNGPAGNLFTPYLAV
                     ISVLSMFYGSIVAIAQSNIKRMLAYSSISHAGYLAIGLAAGNHDAVAGVVFYLAAYTF
                     MNLGAFGIISLVEGKDESNLDISSYAGLGNRKPLLAALLSVLMFALAGIPPFAGFFGK
                     YYIFIAAIKAHLTWLAILGVLSSAISVYFYLRVVVLMYFKTSEDDFAIEPSNNSMLAI
                     IFSVLVVILLGVFPGTFLDLVTRFI"
     gene            complement(5009..5374)
                     /locus_tag="VF870_09020"
     CDS             complement(5009..5374)
                     /locus_tag="VF870_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252371.1"
                     /translation="MKTFKDALSKNMIGIYYGNRVLLPFHCHILKIVIENNIITDFSP
                     NNKGAFINEYPEYTEIYFLDYPDLEEKITKYEVIKMVIVEKDQDLFDFNKHLKISLHL
                     DENHRLLIKEIDENTILID"
     gene            complement(5375..5509)
                     /locus_tag="VF870_09025"
     CDS             complement(5375..5509)
                     /locus_tag="VF870_09025"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX9252372.1"
                     /translation="MVRSISERSGIKMPSFFGYLFKYAVPVLLPIFTLVWLVFYYGKV
                     "
BASE COUNT         1950 a         1008 c          785 g         1776 t
ORIGIN      
        1 taagactact ccaacattat ttttattaat tttgagtaag cctccctgta tagttgattc
       61 tttcccaata ataaaaagaa aaaccattag tagaattatt atcaaagata aaatagaatt
      121 tgcaaatgcc ctaaaaagta tgattttctt aaagttaaaa atgcttaacc agactaaaaa
      181 aatcattatc aggaatgtta gcgtataaat aaaaaaaaca gcaccaatga ataattcatc
      241 gccggtctgc cccagataat agaacttttt aaatggtaaa ggtatgtact ttaaaaaata
      301 aagagtaacc aatataaatt gatttattag aaagactggg ataaatattt tcagattaaa
      361 tacaattgat tttttaaaaa tgataaccag gagtccaatt ataatcaaaa gcacaaataa
      421 tagatttata atattaccaa aataaaaaat attgaaatca cgaagagtta agttttggtt
      481 gttatattta ttaaagtaaa gaaaggccaa atctgctgta atagcaagaa taataaagac
      541 aggtaggaat tttctttctc ttttcttatt ctttgccatc atttcaatga attttttttt
      601 gcctgattaa agtgttaata gccaaaaata ctaattaaat atattgaaaa aaatcacatt
      661 aagatgatta gtaggctgga aattttaaaa ttattaaaat taaaaaggcg tctttaagac
      721 gcctttaatt tattgaagtt taaatacaac aggtattgaa acctgaactc ttactgcctt
      781 acctctttgt ttacccggtt taaatttagt tttcttaacc gcatccaaag ctgcctcatc
      841 acaaccggca ccaattcctt taataatttt tgcatcagta actgttccag attcatctac
      901 aaaagcgaga acataaactt taccttcaac accagctctt ttagctattt cagggtaaac
      961 tatctttttc tgaatttcac taattccgcc tattggctcg ggcatctctt ctactgccac
     1021 aaagtaaact ggttcttctt caacaacctt tttgtcttct tttggaggag gtggaggtgc
     1081 atccatttgt tcatttatat caatttcagt atcaccaatt tctatatctt ccaacacatc
     1141 cgaagaaggt gcttcaatcg ggataggagg ttttggaggt ggtggtggtc tattttcctg
     1201 tttagtctga tcgatctgct caacctggaa taattcctgc ggaccttcag ttacgacttc
     1261 ctttccctga agattgggaa agaacttaaa cgcaacgatt aataatgcca gtgcagctat
     1321 taaactgatt tcaaatactc ttttgtattt agccatcaga tctgcttttt tagattttac
     1381 aattgccatt ttcttttctc ctaacttgca attagtgatg ctaacttaat aatgagatat
     1441 tctattgtca aattattttt caagtctttt acaataataa ttttaaaaag ttcccgcttt
     1501 taatttgctt ttttcaattt cattttgttt gatcttctta accagatatc tggttataag
     1561 aactgctgtc aaagatccta aaaaatccgc cagccaatcc aataattcag cacttcttcc
     1621 cggaacaaat aattgatgca attcatcaag cagaccataa atagacgcaa taataactgt
     1681 gaatgttgcc ggatattttt ttaagagtgc aaatttttct tgtaaaaata aatttagata
     1741 tagaaacacg cctaacaaac catatgctcc aaaatgttca attttatcgc tcacatccat
     1801 atttatagcc attgaggatg gcaaacttgt taggataagc aaaataatcc aatagattac
     1861 aagtggaaca ttaactatat aaaaacgata cttctgcaga aaactataca atacttgcct
     1921 ctaaaaattt tattgcagca ggataattat tcattgtatc agatgcaaga taatttgaga
     1981 tcggtttttc tgtaagtagc aggtctgctg ataagctgtg taaatatact ccggcaattg
     2041 ccgctgcttc aggattttta ctctgagata ataaccccac aattaaaccg gtaagtacat
     2101 caccagaacc aaactttgcc attccgctgt tgccggttgt attaataaat gcttcacctc
     2161 caggagtgaa aataattgtt gatgcacctt ttaatactaa aatacattta tgcttttctg
     2221 caaaatcttt tccgtaactt aaaatatctt tttcaatcag ttttgcttca atcccaatca
     2281 gttgagagaa ctctccaatg tgtggagtta aaatacagtt ttcaagatta caatggttaa
     2341 gaaattcttt atccagggca aatagagcat ctgcatcaat aacagcaaaa ttaaattttc
     2401 tttcaaccac aaatctttta actgcaatta tagtttccgc ctccctgcct aaaccaggac
     2461 caattgcaac aacatccgcc catttaattt tttgctgaag agaatctaat ccatcattat
     2521 caaaatattt tgttttttca ttgccataag attcaacaac aacctcaagt aaatttttat
     2581 ggataagctt ttttgttgat tcaggaattg aaagtattga agctcctgcg ccagaaatca
     2641 atgctgattg agatgttagc gaagctgcac cgggatattt aaaagagccg gctattgata
     2701 acacttttcc agcagaatat ttatgaactg ttttacctct tttgggaaga gaagaataaa
     2761 catcttccgg ttcgattaaa taggtatcag gataaatatc atcaaaataa tctctgccta
     2821 tgccaatttc cttcaaaaca acttcaccgc aaaattcata tcccatgccg atgaacaaac
     2881 cttttttaaa ctcaccaagc gtaatcgtta aatcagaatt gaaaactata ttaccaaagc
     2941 tcgtatcggc atttaatcct gttggaacat caatcgcaca tttaattgta cttattttat
     3001 tcaaagactt tataattgaa gcataaggtt cttttaattc acccgtaaat ccgctgccta
     3061 aaattgcatc aattataaca tcgcagtctt ttaaccagct aatatctttt atggatttaa
     3121 atgattttag aagaagattt tttctttgtg atgaaagatt tttgagaata ttaaagtttg
     3181 ttttacaatc ttcacttaaa tctttttctg agccaagtga aattacttta acatcaaacc
     3241 ccgaatttga aaaatgcctt gcaactgcat agccatctcc accattatta ccctttccac
     3301 aaacaactcc aacacttttt agattatcaa aatgctccaa aattgattga taaataccta
     3361 acgcagcatt ttccattaaa acaattcctg gtgcgtttaa tctttttata gcaaacgaat
     3421 caagattacg aatttgtgat gtgttataaa gtggtatcat acattttttt aataagctat
     3481 tgtgaggagt ttacaacgga gcaatctatt ttattaacta agtattaaga tcacttcgtt
     3541 ttgctcatga tgacaattac aatttaaaat tagctgcttt aaataaacct tgtcaccaag
     3601 tctaaaaatg ttccaggaaa aacaccaagt aaaatcacaa caaggaccga aaaaattatt
     3661 gcgagcattg aattattact tggttcaatt gcaaaatcat cttccgatgt tttgaaatac
     3721 atcaacacaa caacacgcaa atagaagtaa acacttattg cactggatag aacaccaagt
     3781 atcgccagcc aggttaaatg agcttttatt gctgcgataa aaatataata cttgccaaaa
     3841 aaacctgcga atggtggaat accagcaaga gcaaacatca gcacagaaag gagtgccgct
     3901 aacaatggtt ttctatttcc taatcctgca taagaactaa tatcgaggtt tgattcatct
     3961 ttaccttcga cgagagaaat aattccaaaa gcaccaagat tcataaaagt gtaagcagct
     4021 aaatagaata caactcccgc aacggcatcg tgattgcctg ctgccaaacc aattgctaga
     4081 tagcctgcat gagagattga agaatatgcc aacattcttt taatattgct ttgagctatt
     4141 gcaacaatac ttccatagaa cattgataaa acagaaatta cagcaagata tggagtaaat
     4201 aaatttcctg ccggcccatt aaacaatgca aacaatgttg caattattgc gctgaaagca
     4261 gcggctttgc cacttgttga aaataaggca gcaacagtag ttggagagcc ttcataaaca
     4321 tcaggcaccc acatgtgaaa tggaaacgct gcaatcttga atgaaaatcc gactaaaaac
     4381 aatagtactc cggcaacaaa taacaaattc ttagtcaaat ctgcaaagtg caaggttata
     4441 aaatcaattg aagttgtctg agcactgcca taaatcaacg ctattccata aacaataaat
     4501 cctgttgcaa aggcaccaag taaaaaatat tttagtgaag cttcatttgc attaagtttc
     4561 tttctgttca ttcccgctaa gatgtagaat gaaactgaca ttaattctaa tccaagaaat
     4621 attacaaaca aatcttttgc tccagccatt agcatcatac cgagcacaga agattgcagt
     4681 attatataat actccccaaa ataagttcca tattttttta tataatcaat tgaaagcaag
     4741 caaactactg cagcacccaa agtaaatatt gcataaaaaa catttacgtt gccgccggtt
     4801 gcaagcatat tttgcataac tattgataca tcgccaacgt aatacaagga atgtaaaccc
     4861 gctgccagga ataaaagcac agaaaaccat ggaagcaaaa attcactttt ctttgagtac
     4921 atttcaatta aaagcgagat cactattccg gttccaatta atactaatgg aagaatgcta
     4981 aaaaactctt gaaaattatt aatcataact aatctattaa aattgtgttt tcgtctattt
     5041 ctttaattaa aagtctatgg ttttcatcaa gatgtaagga aatttttaga tgtttattaa
     5101 aatcaaaaag atcttggtcc ttttcaacaa ttaccatttt aattacttca tattttgtaa
     5161 tcttctcttc caaatcagga tagtctaaaa aataaatttc tgtgtattcc ggatattcat
     5221 ttataaatgc acctttatta ttcggactaa aatcggttat tatattattt tcaataacga
     5281 tctttaatat atggcaatgg aacggaagta aaacccggtt accataataa ataccaatca
     5341 tatttttgct taatgcatct ttaaatgttt tcatttatac ctttccataa taaaacacta
     5401 accaaaccaa agtaaaaata ggaagcagga caggaacagc gtatttaaat aagtaaccaa
     5461 aaaaacttgg catctttatg cctgaccgct cactgataga tcttaccata aagtttggc
//
LOCUS       DASWBY010000502         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_657907, whole genome shotgun sequence.
ACCESSION   DASWBY010000502 DASWBY010000000
VERSION     DASWBY010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657907"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VF870_09030"
     CDS             complement(<1..459)
                     /locus_tag="VF870_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252373.1"
                     /translation="MDDELKKEIEQSLKKLKPHTKFDEFKKWIPSIIILPIAIYWVLN
                     RGQYGIIDHADLVIHEAGHLFFRFFGKYIYTLGGTLMQIILPSIIFFYFFRNDYRTGM
                     QVSLLWLGQNFINISVYAADAQARRLPLLGGNKVYHDWHYLLSEIGLLQYD"
     gene            563..1393
                     /locus_tag="VF870_09035"
     CDS             563..1393
                     /locus_tag="VF870_09035"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9252374.1"
                     /translation="MRKTKFIFSLILIFTVISFTQDFKTPRPSPDATVSQYVGVTNIT
                     VDYSSPAVKGRKIWGELVPFGQVWRTGANEATTITFTDAVSINGNELAAGTYGIHSIP
                     NANEWEIIFSKDTPVDGSSTFDPKKEVLRIKVKPEEHHFMERMTFLFKDATENSVNVN
                     LVWEKLKVSFKVDVKTQELTLQKARDAFKWNQLMAAATYSLEQNTNLDEGFKWIQASI
                     LINENYWNTRILAQYYSKMNKKADAISTMEKAIDLGRKMQSAPFDFDRMKQMLADWKK
                     "
BASE COUNT          553 a          270 c          265 g          468 t
ORIGIN      
        1 atcgtattga agcaaaccga tctcactcaa caaataatgc caatcgtggt agactttatt
       61 accgccgagc aatggtaatc ttcttgcttg tgcatctgca gcgtaaacag atatattgat
      121 aaagttttga ccaagccaca atagtgaaac ctgcattccg gttctataat catttctaaa
      181 aaagtaaaag aaaattatag atggcaaaat tatctgcatt aaagttccgc cgagagtata
      241 gatgtatttt ccaaagaacc taaagaacaa gtgtcccgct tcgtgaatta ccaaatctgc
      301 gtgatctatt attccatatt ggccgcgatt taatacccaa taaatagcaa ttggaaggat
      361 aataattgat ggaatccatt ttttgaattc atcaaattta gtgtgcggct taagtttctt
      421 tagactttgt tcgatttctt tttttagttc atcatccatt gaattgctta aataaaaaat
      481 gatctttaac ttcgcatcga agttaactaa tccaaaaaca ccgttcaatc gtttattaat
      541 aaataaaatg aaagggtaca ctatgagaaa aactaaattt attttttccc tgattttgat
      601 ttttaccgtt atatctttca ctcaggattt taaaacccca cgccctagtc cggatgctac
      661 ggtttcacaa tatgtcggag ttaccaatat aactgtggat tacagcagtc cggcagttaa
      721 aggcagaaag atttggggtg agcttgttcc ctttggccag gtttggagaa ccggagctaa
      781 tgaagcaaca acaattacat ttacagatgc tgtaagtatt aatggaaatg aacttgctgc
      841 gggtacatac ggaatccact caattcccaa tgcaaatgaa tgggaaataa tttttagtaa
      901 agatactccc gttgatggaa gttcaacatt tgatcctaaa aaagaagtgt tacgcataaa
      961 agtaaaacca gaagaacatc attttatgga aagaatgacc ttcttattta aagacgctac
     1021 ggaaaattca gttaatgtaa atcttgtttg ggaaaaatta aaagtatcat ttaaagttga
     1081 tgtaaaaact caagagctta cattacaaaa agctcgcgac gcgtttaaat ggaatcaatt
     1141 aatggcagca gctacatatt ccctggagca aaacacaaac ctagatgaag gatttaaatg
     1201 gattcaggca tctatcttaa taaacgaaaa ctactggaat acaagaatac ttgcacagta
     1261 ttacagtaag atgaataaaa aagcggatgc gatatctact atggaaaaag caattgatct
     1321 tggtagaaaa atgcaaagtg ccccatttga ttttgatagg atgaaacaaa tgttagctga
     1381 ctggaaaaaa taaaacaatc acgttcttag cgtctctgta tcaacactaa atgcggagac
     1441 gcaaaaacgt tacactatcc attataaact tttctcttat ttgaacttga tctgcattca
     1501 tcgctacagc aatattcttt ttcaacttta caattgtggc aacaaacaat aatttt
//
LOCUS       DASWBY010000503         7826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_734765, whole genome shotgun sequence.
ACCESSION   DASWBY010000503 DASWBY010000000
VERSION     DASWBY010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734765"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /gene="alaS"
                     /locus_tag="VF870_09040"
     CDS             complement(<1..1038)
                     /gene="alaS"
                     /locus_tag="VF870_09040"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX9252375.1"
                     /translation="MTSRQIRQQFFDFFKSKEHRIVQSSPVVPFDDPTLLFTNAGMNQ
                     FKDVFLGTGTREYKRAADTQKCIRVSGKHNDLEEVGHDTYHHTLFEMLGNWSFGDYYK
                     KEAIKWAWELLTEVWKLPKERLWATVYKDDDEAFELWKSETDINPAHILKFGEKDNFW
                     EMGDTGPCGPCSEIHVNISDDLENAELVNAGSPECIEIWNLVFIQYNRDEKGILHELP
                     AKHVDTGMGFERVCAVLQNKKSNYDTDVFTPIIDAVVQLSKVKYDKEEDRIPMRVIAD
                     HIRALTFAIADGAVPGNDGRGYVLRRILRRAARYGRKINLKDPFLYKLVDVLAETMGD
                     VFPEIIEKKDYV"
     gene            1193..4222
                     /locus_tag="VF870_09045"
     CDS             1193..4222
                     /locus_tag="VF870_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX9252376.1"
                     /translation="MSESIKTTDNELLLQIVGYNSEAFEQLYNRYSATVYSLIKEIVT
                     NPKLAEKILLNVFSVFLKRIDYFSTEGNDIFTCFTLLARNISIDSLKRMKFVEGIPEY
                     SDKYEVEFILPNISKVINPINFDERNALGEKIKAYKNHLTEVQNLVLSLVYFEGLDEE
                     EIAKRLSVPSATVRQKILSIVDSLYQQYTGKNGDSNNDKKVSPLIKLEALGCLSSEER
                     ILLNNFRKNDPDFLWKELGEYQNLTALISTAIPIENPGHELQNEIKNIFIKISGGGEI
                     DYKIVAPEPIVVSQVEKPKLEPVKQKIENSIKEIVQKPILNEISNHAPKPIPESVQQK
                     IQNSIPETNKQPEIKEKKKPEFELKFRERDPEELSLLRKLERDELNKKTSNTLLKTNS
                     DVKLKDHNPPLNKPNVFSDKNPVVSSEKKSTPAAPIMETGINNKTTAATENKTELKID
                     NDDPSIVIDQPKTLTNIEEVVVKNRLIPNSSINLKELFKKDTEPVLNKENTTVNLKEE
                     TKAERKPITQVVEKSEIKIKTNEPTKEFTTANVFIDKLEKIIENKPADPAREELSLEN
                     KSTEPAKENRVTNTIAAKEEKNVEIKIKSNESPQELRRSNVFVDKNQKGTPAKQPVLV
                     AKKHELESKKEEPVKEIQKKNVVEEKADILKSAAQISLNPNEKIKSQVKVDENLSNLK
                     KPAVERSNLKIRETVFTENEEKPEIINDRIKSRVAISKSEIKKTETPDKLTKEINIDE
                     ILSKIDDEKPSSALSETESYEKEIVSLNKKVRKNILAFAAMFVIVVSASVFVYLQFQE
                     APVKAANKINNPEKINLAGQTNLVLNNENVPEVETENTVKETESVNTEKPLVKQPAVI
                     PPLPEISIKEESTLFASNVNEGLMNDLNTETHQTAAAKTETVVPPKENKVVDEEPAFF
                     VAVEEMPELIGGLKGLQSKIIYPEIAKRVGIEGKVVVQALVDEAGNVISVGTLKGIGS
                     GCDEVAMDAVRNSKFIPGKQRGKNVKVQVNIPIIFKK"
     gene            complement(4338..7730)
                     /locus_tag="VF870_09050"
     CDS             complement(4338..7730)
                     /locus_tag="VF870_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9252377.1"
                     /translation="MKKLILFFVLLITASLTYSQVEVSSRLQQALLTANQNDYIKVLV
                     LLRSQVDLSSLDQQLYDQKSSLQQRAFQVITALKQNAENTQAELKSYLNTKSESAEVY
                     TYEAFWIANMFMVEAKPIIINELMTRLDVSEMDLDALLELDRPEKVEGYVDGIESVEP
                     GLKIINAHLLWAQGITGQGRIVMDIDTGVFPNHPALNFKWRGNHVPSNQAWFDPVGGT
                     TVPSDCDGHGSHTMGTMVGWSPTTGDTVGVAPDAEWIAAKTICTSPHTSNSVAAFQWA
                     INPDGDPLTISDMPDVISNSWYDPDVSNECSGIYKTTLDAVEAAGIAVVFSAGNNGPG
                     TSTITKPKNINTNNVNVMCTAAIDGASYLGGNTNPIASFSSRGPSLCGGTGSLLIKPE
                     VSAPGVSVRSSGSATGYTVLSGTSMASPHVAGAVALLKQFAPNLTGKQILEALYNTAV
                     DLGAAGEDNNYGMGLIDVYAAFLSLGTADSTAPDPITDLSVVSPTSNSLNIQWTVPYD
                     SSMNGVRGYDIRYSTSAITDSTTFYNAAILPYTAIPDTAGAIETYLVEGLAFATPYYF
                     SIKSKDVWGNWSDLSNTATGTTLAAPQISVTPASINNILNPFEVVVDTVTISNISANP
                     STLDFSVALENNTFPDGIISSRIIPKYNQLADAVEVSSKDNPQIFGGVSIDGSGGPDL
                     FGYKWRDSNEPNGPQYVWNDITSSGTLATNWIPTGTYDPKDEGYTGPFPLGFNCKFYG
                     QEKNQIYVSSNGLILFNTINTNIFSNAQIPSAADPNEYIAPFWDDLDGVSSGTVHYKQ
                     EGSTFIIQFTGWHKYSNSGSLTFQIVIHQNGKIVIYYNNMNATLNSATVGIENAAGND
                     GLQVAYNANYVANNLALEFAAEPDWLSNNLNSGTLFNGNSVDVELTFRAEDYPAGNYS
                     MDFNVASNDPVNPTVTVPVTMEISIIPVELTSFVANNDRNNVTLNWATATEVNNSGFQ
                     IERKPNGSNEWISISFVSGKGTSTERSYYSYLDKSLAVGKYSYRLKQVDLDGTFEYSP
                     EIEVDVNAPDEFTLYQNYPNPFNPATTIEYSLPEKADVTISIYTSIGELVTTLVNGTI
                     EAGYQKATFNAANLTSGTYIYQMKAVSSGRTFVDTKKMLLIK"
BASE COUNT         2669 a         1382 c         1419 g         2356 t
ORIGIN      
        1 aacataatct tttttctcaa ttatttcagg aaaaacatcg cccattgttt ctgctaaaac
       61 atctacaagt ttataaagaa atgggtcttt aagattaatt tttcttccat atcgggctgc
      121 acgtcttaaa atcctgcgaa gtacataacc gcgtccatca ttaccaggaa ctgcgccatc
      181 tgcaattgca aaggttagag ctctgatatg atctgcaatt acacgcattg gaatcctatc
      241 ttcttcttta tcgtacttta ctttagaaag ttgaacaact gcatcaatta ttggtgtgaa
      301 aacatcagta tcgtagtttg attttttatt ttgaagcact gcacaaaccc gctcaaagcc
      361 cattcctgta tcaacatgtt ttgcaggcag ctcgtgtaat atcccttttt catctctgtt
      421 atattgaatg aaaactaaat tccatatttc aatgcactct ggtgaacctg catttacaag
      481 ttcagcgttt tcaagatcat cactaatgtt tacatggatt tcggaacaag gaccgcaagg
      541 accggtatcg cccatttccc aaaaattatc cttctctcca aactttaaaa tatgagctgg
      601 atttatatcc gtttcagatt tccagagttc aaaagcttca tcgtcatcct tgtagactgt
      661 tgcccacaat ctttccttag gtagtttcca aacttctgtt aataattccc atgcccattt
      721 aattgcttct tttttataat aatctccaaa agaccaatta cctaacattt caaacaaggt
      781 atgatgataa gtatcgtgac cgacttcttc gagatcatta tgcttgccgg aaacacggat
      841 acatttttgt gtatcggcgg cacgtttgta ctcacgcgtt ccggttccga ggaaaacatc
      901 tttaaactgg ttcattcccg cattggtaaa aagcaacgtt ggatcatcaa acggaacaac
      961 cggcgatgat tgaactattc tgtgttcttt actcttaaaa aaatcaaaaa attgctgacg
     1021 aatttgtcgt gaagtcatat ttacctttgg aaaaattttg aaggtaaaaa taacaaatta
     1081 actactaagc gggaactgtg ttattgaggt ataattgagg atatagtgat gaaaattttg
     1141 gcaaaataat tgcataatta actattctct aaaacaaaaa atgattgcca caatgtcaga
     1201 aagcataaaa acaaccgata atgaattgct gcttcaaata gttggttata actcagaagc
     1261 atttgagcag ctatataatc gatattcagc aactgtttac tcactgatca aagagatagt
     1321 gacaaaccca aaattagctg aaaaaattct tctgaatgtt ttttcagtat tcttaaaacg
     1381 catagactat ttcagcacag aaggcaacga tatttttacc tgctttacgc tgctggcaag
     1441 aaacatttct attgattcgc ttaaaagaat gaagtttgtt gaaggtattc cggagtattc
     1501 tgataaatat gaagttgagt ttattcttcc aaatatttca aaggtgataa acccaattaa
     1561 ttttgatgaa agaaatgcac taggtgaaaa aatcaaagct tacaagaatc atcttaccga
     1621 agtgcagaac ttagttttat cacttgttta ctttgaaggt ttggatgaag aagaaattgc
     1681 aaaaaggtta agtgttcctt ctgcaactgt gcggcaaaaa atattgagta ttgttgatag
     1741 tctttatcaa cagtacacgg ggaaaaacgg ggatagtaat aatgataaaa aagtctcacc
     1801 tttaataaaa ttggaagcat taggatgttt gtcttctgaa gaaagaatct tacttaacaa
     1861 ttttagaaaa aatgatcctg attttttgtg gaaagaactt ggtgaatatc agaatttaac
     1921 cgctctgata tcaacagcaa ttccaataga aaatcccgga catgaacttc aaaatgaaat
     1981 taaaaatatt tttattaaaa tttcaggtgg tggtgagatt gattataaaa ttgttgctcc
     2041 ggaaccaatt gtggtcagtc aagtggaaaa acctaagctg gaaccagtta aacagaaaat
     2101 tgaaaactca attaaagaaa tagttcagaa accaattctt aatgagattt ctaaccacgc
     2161 tccaaagcca ataccggaat cagtccagca aaaaattcag aattcaattc ctgaaaccaa
     2221 taaacagccg gaaataaaag aaaagaaaaa accagagttt gaattaaagt ttagagagcg
     2281 tgatcctgaa gaattgagtt tgttaagaaa gctggaaagg gatgaactta ataagaaaac
     2341 ttcaaataca cttttaaaaa caaacagtga tgttaagtta aaagaccaca atcctccttt
     2401 aaacaaacca aatgtattta gtgataaaaa tccggttgta tcttccgaaa aaaaatcaac
     2461 accggccgct ccaataatgg aaactggaat taataacaaa acaacagctg caactgaaaa
     2521 taaaactgaa ttaaaaattg ataatgatga tccttcaata gttatcgatc aacccaaaac
     2581 attaactaat atagaagaag tcgttgttaa aaatcgacta atacctaaca gcagtattaa
     2641 tctaaaagaa ctttttaaaa aagatactga accggttctt aataaagaaa acactactgt
     2701 taatcttaag gaagaaacaa aagctgagag aaagcccatt acacaggttg ttgaaaaatc
     2761 agaaataaaa ataaaaacaa acgaaccgac aaaagaattt acaacagcta atgttttcat
     2821 agataagctt gaaaagataa ttgaaaataa acctgctgat cctgctagag aggaattaag
     2881 tctggagaat aaatccactg aaccggctaa agaaaatcgc gtcacaaata caattgctgc
     2941 aaaagaagaa aagaatgttg aaataaaaat aaaatcaaac gagtcaccgc aagagttaag
     3001 aagaagtaat gtttttgttg ataagaatca gaaaggtact ccagccaaac aaccagtact
     3061 tgttgctaag aaacatgaat tagagagcaa aaaagaagaa ccggttaaag aaattcagaa
     3121 aaaaaatgta gtggaagaaa aagcagatat attgaaatca gcagctcaga tttcattaaa
     3181 tccgaatgag aaaataaaat cacaagttaa agttgatgaa aatttatcaa accttaagaa
     3241 acctgctgtt gaaagatcaa atctaaaaat aagagagaca gtctttacag agaatgaaga
     3301 gaaaccagaa attattaacg accgaataaa atcacgtgtt gccatatcca aatcggaaat
     3361 taaaaaaact gaaactcccg ataagttgac caaggaaata aacattgatg aaatattatc
     3421 caagatagac gatgaaaaac cttcatcggc gttgagtgaa actgaatctt atgaaaaaga
     3481 aatcgtttcc ctgaacaaaa aagtgagaaa aaacatttta gcttttgcag caatgttcgt
     3541 gattgttgtt tcagcttcag tttttgttta tctgcaattt caggaagctc ctgtaaaagc
     3601 ggctaataag attaataatc ccgaaaaaat taatttagct gggcagacaa atcttgtact
     3661 taataatgaa aacgtaccgg aggttgaaac tgaaaatact gtgaaggaaa ctgaatctgt
     3721 taatacagaa aaacctttag ttaagcaacc ggccgttata ccgcctcttc cggaaatttc
     3781 aattaaggaa gaaagcacat tatttgcctc taatgtaaat gaaggtttga tgaatgacct
     3841 taatactgag acgcatcaaa ctgcggctgc aaaaaccgag accgtggttc cacctaaaga
     3901 aaataaagta gttgatgaag aaccggcttt ttttgttgca gttgaagaaa tgccggaact
     3961 gattggcggt ttaaaagggc ttcaaagtaa aataatttat cctgaaattg caaagcgggt
     4021 tggcattgaa ggaaaagttg ttgtacaggc tttagtagat gaagccggta atgtaatttc
     4081 agttggtaca ctaaaaggca ttggctcggg ttgtgatgaa gttgcaatgg atgcagttcg
     4141 taacagcaaa tttattcctg gtaagcagcg aggaaagaat gtaaaagtac aggtaaatat
     4201 tcctatcata tttaaaaaat aattttgtta acaaacatgt aataaaaaaa ggcacgagta
     4261 atcgtgcctt tttttatttg ttgtcatact tatcgaatct atctgtggtg acgagagtcg
     4321 aagtattaag ataaatttta ctttataagc agcatcttct ttgtatcaac aaaagttctt
     4381 ccactgctaa cggccttcat ctgataaatg taagtacccg atgtaaggtt tgctgcatta
     4441 aaagtcgctt tttgatatcc tgcctcaatt gtaccattca ctaaggttgt aactaattca
     4501 cctatcgacg tataaattga aattgttaca tcagcttttt cggggagtga atattcaatc
     4561 gtggttgccg gattgaatgg atttggataa ttctgataca aagtaaattc atcaggcgca
     4621 tttacgtcta cttcaatttc cggtgaatat tcaaatgttc cgtcaagatc aacctgtttt
     4681 aatctgtatg aatattttcc aacggctaaa ctcttatcca aataggaata ataacttctt
     4741 tctgtagaag tacctttacc tgaaacaaat gagatgctaa tccattcatt cgatccgttt
     4801 ggttttcttt caatctggaa cccgctgtta ttaacttctg tagcagttgc ccagtttaag
     4861 gtaacattat ttcgatcatt atttgcaaca aaagatgtca gttccacagg gataattgag
     4921 atttccattg taacaggaac ggtcactgtt ggatttacag ggtcattact tgcaacatta
     4981 aagtccattg aataattgcc tgcaggataa tcttctgcac ggaatgttaa ttcaacatca
     5041 acagagttgc cgttaaataa agtaccgctg tttagattgt ttgataacca atctggttct
     5101 gctgcgaatt caagcgctaa attatttgca acatagtttg cattataagc cacctgtaat
     5161 ccatcatttc cggcagcatt ttcaattccg actgttgcgg aattgagagt tgcgttcatg
     5221 ttattatagt aaataacaat ctttccattc tgatgaatca caatctggaa agtaagcgag
     5281 ccagagtttg agtatttgtg ccatccggta aactgaataa taaatgttga tccttcttgt
     5341 ttataatgaa ctgttcctga agaaacacca tcaagatcat cccagaaagg cgctatatat
     5401 tcatttggat ctgctgcact tggtatctga gcatttgaaa aaatattagt gttaatggta
     5461 ttaaacagaa ttaaaccatt agaacttaca taaatctgat ttttttcctg accataaaat
     5521 ttgcagttaa atccaagtgg gaatggcccg gtatagcctt catctttagg atcgtatgtt
     5581 cccgttggaa tccaattagt tgcgagagta ccgcttgaag taatatcatt ccagacatat
     5641 tgcggtccgt tgggttcgtt actgtctctc catttgtatc caaataaatc cggtccgccg
     5701 cttccatcaa tactgactcc accaaagatt tgcggattat cttttgatga tacttcaact
     5761 gcatcagcaa gttgattgta tttaggaatt attcttgagc taataatacc atctggaaaa
     5821 gtattgtttt caagcgcaac ggaaaaatcc aatgtggatg gattagcaga gatattagaa
     5881 attgttactg tatcaacaac cacttcaaaa ggattcagta tattatttat acttgcgggt
     5941 gttactgaaa tttgtggcgc agctaacgtt gtacctgttg cagtgttaga taaatctgac
     6001 cagttgcccc aaacgtcttt ggatttaatt gaaaaataat acggtgtagc aaaagccaaa
     6061 ccttcaacaa gataagtttc aattgcgcct gctgtatcag gtattgctgt gtagggcaaa
     6121 attgctgcat tataaaaagt tgtagagtct gtaattgccg atgtggaata tctaatatcg
     6181 tatcctctta caccattcat ggatgaatca taaggaacag tccattgaat atttaatgag
     6241 tttgaggttg gggaaaccac acttaaatcg gttattggat cgggagctgt cgaatcagcc
     6301 gttcctaaac ttaagaaagc ggcataaaca tcaatcaatc ccattccgta gttattgtct
     6361 tcacctgctg caccaagatc aacagcagta ttatataggg cttcaagaat ttgcttgccg
     6421 gttaaatttg gtgcaaattg ttttagtaat gcaacggctc ctgcaacgtg aggtgaagcc
     6481 attgaggttc cgcttaatac agtataaccc gttgctgagc cggaagaacg aacacttact
     6541 cccggggcag aaacttcagg tttgattaat aatgaaccag tgccgccaca aagcgatgga
     6601 ccgcggcttg aaaagcttgc tattgggttt gtatttccgc caagataaga tgcaccatca
     6661 atagcggcgg tacacattac gtttacatta tttgtattaa tgtttttggg ttttgttatt
     6721 gtagatgttc cgggaccgtt gttgccagca gagaaaacga ctgcaatgcc ggcagcttca
     6781 acggcatcga gtgtggtttt ataaattcct gaacattcat tggatacatc aggatcgtac
     6841 cagctgttgc tgataacatc tggcatatcg gatattgtca agggatctcc atctggatta
     6901 atcgcccatt gaaaagcagc aaccgaatta gacgtatgcg gacttgtaca aattgttttt
     6961 gctgcaatcc attcagcatc aggagcaaca cccacggtat cgccagttgt tggagaccaa
     7021 ccaaccattg ttcccattgt atggcttcca tgtccatcgc aatcacttgg aactgtagtt
     7081 cctcctacag gatcaaacca agcctgatta cttggaacgt gattacctct ccatttaaag
     7141 ttaagtgcag gatgatttgg gaaaacaccg gtatcaatat ccattactat tctgccctga
     7201 ccagtaatcc cttgtgccca caaaaggtga gcgttaataa tttttaatcc cggctcaaca
     7261 gattcaattc catcaacata gccttccact ttttcaggtc tatctaattc tagaagagca
     7321 tcaagatcca tttccgaaac atctaatctt gtcataagtt cattaataat aatcggttta
     7381 gcttctacca taaacatatt tgcaatccag aaagcttcat aagtatatac ttcagctgat
     7441 tctgattttg tatttaggta tgattttaat tcagcctgag tgttttcagc attctgttta
     7501 agagcagtaa taacctgaaa tgctctttgc tgtaatgagg atttttggtc gtacagctgc
     7561 tgatcgagag aacttaaatc aacttgtgat cggagaagaa cgagtacttt gatgtaatca
     7621 ttctgattgg ctgttaacaa agcctgctgt aatcttgagc ttacttcaac ttgtgaatat
     7681 gtaagagaag cagtaatgag cagaacgaag aatagtataa gttttttcaa tatatcactc
     7741 ctttatgttt tacgttcaaa tttaacatgt aattattaat gcttcaacca tttagaaaat
     7801 ttaagttaat gactgtaatt ttgtta
//
LOCUS       DASWBY010000504         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_23334, whole genome shotgun sequence.
ACCESSION   DASWBY010000504 DASWBY010000000
VERSION     DASWBY010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23334"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /gene="mreC"
                     /locus_tag="VF870_09055"
     CDS             complement(<1..484)
                     /gene="mreC"
                     /locus_tag="VF870_09055"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX9252378.1"
                     /translation="MIRFFVSVWENFKEYIVLVVLLIVSLVLLSQNNSQGIQKVRSIA
                     FGTFASVTSVFTDIFNISNLKSENENLRRTNAEMMLQLSRLREYAIVNEDLKGLLALK
                     DSSAFPLIPATIVSKSLTKSQGTITLNAGKNQSVKVGMPVITDKGLVGIIYSISDNYS
                     I"
     gene            complement(484..1512)
                     /locus_tag="VF870_09060"
     CDS             complement(484..1512)
                     /locus_tag="VF870_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9252379.1"
                     /translation="MGIFDFFSNDIAIDLGTANTLIHVKGKGIVLNEPSIVAFDKNSK
                     RIIALGNKAKEMQGREHKEIKVTRPMRDGVIADFEIAEGMIRAFIKRVHGGSVMSSRR
                     VIVAVPSGVTEVEKRAVRDSAEHAGAKEVHLIAEPMAAAIGIGIDVEAPVGNMIIDIG
                     GGTTEIAVIALSGIVNEESIRIAGDEMNYAIMQFFKKNHNILIGERTAEAIKCEVGSA
                     VPLKEEITIQVKGRDLVGGIPKTTEVSSVEIREALNEAVVQIVDAVRQTLERTPPELS
                     ADILDRGVMLTGGGALLKGLDERIRMETNLPVHVAEDPLTAVVRGAGKVIDNLNHYSK
                     VLIRNRRY"
     gene            complement(1522..>1927)
                     /gene="purH"
                     /locus_tag="VF870_09065"
     CDS             complement(1522..>1927)
                     /gene="purH"
                     /locus_tag="VF870_09065"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857481.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX9252380.1"
                     /translation="LKEDELKIVTDKKPSKEELEDLKFAWKIAKHTKSNAIVYVKDKA
                     TLGVGAGQMSRIDSAKIAVMKAKEHGLDLTGSVAASDAFFPFADGLIEIIKCGAVSVI
                     QPGGSVRDQEVIDAANQNKISMVFTGIRHFKH"
BASE COUNT          561 a          416 c          297 g          653 t
ORIGIN      
        1 caatggaata attatctgat attgaataaa taattccaac taaacctttg tctgtaataa
       61 caggcatccc gacctttaca gattgatttt tgccggcatt aagagttatg gtaccttgag
      121 attttgttaa tgattttgaa actattgttg caggaataag cgggaatgca gaagaatctt
      181 ttaaggcaag caaaccttta agatcttcat ttactattgc atattcacgc agtctgctta
      241 attgcagcat catctccgca ttcgttctgc gcagattttc attttcactt tttagattac
      301 tgatattgaa aatatctgta aacactgagg taactgatgc gaatgttcca aacgctattg
      361 agcgtacttt ttgaattccc tgtgaattgt tttgtgagag cagaacgagg ctgacaatta
      421 ataaaactac aagtacaata tattccttaa aattttccca tacagaaaca aaaaatctta
      481 tcatcaatat cttctattac gaattaatac cttagaatag tgattaagat tatcaataac
      541 tttacctgcg cctcttacaa ctgcagttaa tggatcttct gcaacatgta caggaagatt
      601 agtttccatt ctgattcgtt catctaatcc ttttaacaat gcaccgccgc cggtaagcat
      661 aacacctcga tcaagaatat ctgcagaaag ttctggcggt gtgcgttcaa gagtttgtct
      721 aactgcatca acaatctgaa ctacggcttc gtttaaagct tctctaattt ctactgaact
      781 aacttctgtg gtttttggaa tgccgcctac taaatcacgt cctttaacct gaatagtaat
      841 ttcttctttt aatggaactg ctgaacccac ttcgcattta attgcttctg ctgtcctttc
      901 gccaattaat atgttatgat tttttttgaa gaactgcatg atagcatagt tcatctcatc
      961 accggcaatt cggattgatt cttcatttac aattcccgat aaagctataa ctgcaatttc
     1021 tgtagtaccg ccaccgatat caataatcat atttccaaca ggagcttcaa catcaattcc
     1081 aattcctatt gccgcagcca ttggctcagc aattaagtga acttcttttg caccggcatg
     1141 ttctgcacta tctctaacag cgcgcttttc aacttcagtt actccgctag gcacagcaac
     1201 tataactctt ctgcttgaca taacactgcc gccatgcacc cgtttaataa aagcacgaat
     1261 cattccttcg gcaatttcaa aatcagcgat aacaccatca cgcatagggc gcgtaacttt
     1321 aatttctttg tgttcccttc cctgcatttc tttagcttta tttccaagtg caataatccg
     1381 tttggaattt ttatcaaatg caacaatgga aggttcattc aatacaatcc ccttaccctt
     1441 aacatggata agagtgtttg ctgttcctaa atcaattgct atatcattcg agaaaaaatc
     1501 aaatattccc attgttcctc tttaatgttt aaagtgcctt attccggtaa ataccataga
     1561 aattttattt tgatttgcgg catcaataac ttcctgatcg cgaactgatc cgccgggttg
     1621 aatgacagaa actgcaccgc atttaattat ctcaatcaga ccatcagcaa aaggaaagaa
     1681 tgcatcggaa gctgcaacag agcctgttaa atcaagtccg tgttctttag ctttcatcac
     1741 tgcaatcttt gccgaatcta ttctggacat ttgacctgcg ccaactccta atgttgcttt
     1801 atccttgaca taaacaattg cgttggattt tgtatgttta gcgattttcc atgcaaattt
     1861 taaatcttct aattcttctt ttgatggttt cttatcagta acaattttca attcatcctc
     1921 ttttaag
//
LOCUS       DASWBY010000505         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_734849, whole genome shotgun sequence.
ACCESSION   DASWBY010000505 DASWBY010000000
VERSION     DASWBY010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734849"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="VF870_09070"
     CDS             <1..1592
                     /locus_tag="VF870_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252381.1"
                     /translation="NKYFFTILILITFAGNYFAQPNIRIEPRNIRFVDVFNRYNYAII
                     YNDGNQPLTIDSLSPTKPYYILDFEGHPQLPFIISPGDTIKLNVTLTNFYNITVSDTT
                     DTIWVYSNDPESPRDLRIKIDFFDDDYGNCTGTITDEAITPLPDTKIYFLYYGIYLFD
                     STNTDISGNYSIQLPKGNYIVAAEREGYRVMFSGNTPDPFFAQTIPLDSGQSVIVDLA
                     LPVVNNSGYSVSGVVIDSVYGVPLDKGVVIIRRGTHTPTLLKPNGTATDTTVYAGFIK
                     SDGSYRISVEDSTYYFVQGYSEYYLPTYFNDKNAASVFWQNADSVFVNQTLANKNLYL
                     ERDSAYGGGGAYGNITLPFSEAQGFDGVTVAAKSLNNNQLYAYNFGKEDGKFYINNLP
                     YGTYQLVAQKVGYPNALSSEFTISPQNQIQYNLNLHFTLTDVEVEENLIPIDIKLYPA
                     YPNPFNPSTTISFSLPSAQLVKLIVYNILGQKVAEIGNKDFAAGLNRVSFNAQGLSTG
                     IYFITLETKSSRLSQKIILMK"
     gene            1855..>2439
                     /locus_tag="VF870_09075"
     CDS             1855..>2439
                     /locus_tag="VF870_09075"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX9252382.1"
                     /translation="MKTQQTASSYNLQAISKILLVTLIMFISNITLFAAHDIEVTGNI
                     TELGSDYLVVQGYTIYVDEYTELRGPNNTTVIFSFFQLNDLVQVQADSRGDGTYLAAR
                     VKSDDGAGGGNENEVELTGYVTEVGTSSFTINGTVFFVDVNTEYRGRHGNTFSLEKIV
                     PGILLDVKATLQTNGDLLATRVKTEDDENQHESEL"
BASE COUNT          831 a          422 c          421 g          765 t
ORIGIN      
        1 tgaataaata tttttttaca attttaattt taataacatt tgccggcaac tattttgccc
       61 agcctaatat acgaatcgaa cctagaaata ttcgatttgt agatgttttt aataggtaca
      121 attatgcaat aatatataat gatggaaatc agccacttac tattgacagc ttgtcaccta
      181 ctaaaccata ttacatttta gattttgaag gacatccaca attacccttt attattagtc
      241 ccggagacac aataaaatta aatgttactc ttacaaattt ttataatata actgtaagtg
      301 atacaactga cactatttgg gtatacagca atgatcccga aagtccgcga gatttaagaa
      361 taaaaattga tttttttgat gatgattatg gaaattgtac gggaacaatt accgatgagg
      421 cgataactcc cttaccggac actaaaatat attttcttta ttacggaatt tatctttttg
      481 attctactaa tacggacata agcggaaact acagcataca gctaccgaaa ggtaattata
      541 tagtggcggc tgaaagagag ggatacagag taatgttttc tggtaatacc cctgatccct
      601 ttttcgcaca aaccattcca cttgactcag gacagagtgt tattgttgat ttagctcttc
      661 ctgttgttaa caattccggg tattcagtct ctggagttgt aattgattca gtttatggag
      721 ttccgctgga taaaggagtt gtaattattc gcagaggtac acacacaccg actttactta
      781 aaccgaatgg gacagcaact gatacgactg tttatgcagg ttttataaaa tctgatggaa
      841 gttatcggat atcagttgaa gacagcacat attattttgt tcaaggatac tccgaatact
      901 atctgccgac atattttaat gataaaaatg cagcatcagt tttctggcag aatgcagatt
      961 cggtctttgt taatcaaact ttagctaata aaaatcttta tttagaaaga gactcagcct
     1021 atggcggagg aggcgcgtac ggaaatatta cacttccatt ttcagaggca cagggatttg
     1081 atggagtaac tgtggctgcg aaatctttaa acaataatca gctttacgct tataattttg
     1141 ggaaggagga tggaaagttc tatataaata acctgccata cggtacatat cagcttgtgg
     1201 cccaaaaggt tgggtacccc aatgctttaa gcagtgaatt tacaattagt cctcaaaatc
     1261 aaattcaata taatctgaat ttacacttca cattaaccga tgttgaagta gaagaaaatt
     1321 taattcctat tgatataaaa ctatatcccg cttaccccaa tccatttaat ccgtctacaa
     1381 caatttcttt ttcactcccg agtgcacagc ttgtgaagct gatagtgtac aatattttag
     1441 gacaaaaagt tgccgagatt ggtaataagg attttgcggc cggattaaat cgagtgagct
     1501 ttaacgcaca aggattatca acaggaattt attttattac cctagaaacc aaatcatcca
     1561 gattatcaca aaaaattatt ttgatgaaat agaagttcta ttaaataatt tttcgtcttc
     1621 ctatttattt aaccgataaa ccgaaacaaa agcgtttcgg tttttttatt aataaaatag
     1681 caattcaatt ttttgacaac tcattcaatg ctatccagtt taattctatt aaaaaatttt
     1741 agataaagat taacaatggc atgtcacctt tatgttatga cacgcactta ttatctaaag
     1801 agcatacatt aaataaaaat caataaaatg aatctatcaa aaaaggagta cacaatgaaa
     1861 acacagcaaa cagcaagcag ctataattta caggctataa gcaagatttt gcttgtaaca
     1921 ctaattatgt ttataagtaa cataacacta tttgccgctc acgatattga agttaccggc
     1981 aatataacag aacttggctc tgattactta gttgttcaag gttatactat ttatgttgat
     2041 gaatatacag aactccgcgg gccaaataac acaaccgtta tcttttcttt cttccagtta
     2101 aatgaccttg tacaagtcca ggctgatagc agaggcgatg gaacttatct tgcagctaga
     2161 gtaaaatccg atgacggtgc cggaggtgga aatgaaaacg aagtcgaatt aaccgggtac
     2221 gtaacagaag tcggtacctc atccttcaca attaacggta cagtattttt tgttgatgta
     2281 aatactgaat atcggggcag acatggaaat actttttctt tagaaaaaat tgttcccgga
     2341 attctgctcg acgtaaaagc gactttacaa actaatggag atttactagc tactcgtgtt
     2401 aaaaccgaag atgatgaaaa tcagcatgaa agcgaactt
//
LOCUS       DASWBY010000506         4939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_356822, whole genome shotgun sequence.
ACCESSION   DASWBY010000506 DASWBY010000000
VERSION     DASWBY010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356822"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..741
                     /locus_tag="VF870_09080"
     CDS             166..741
                     /locus_tag="VF870_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX9252383.1"
                     /translation="MSDLEYFTDKEFSNINFSKSRFTPGEYENCKFEKCAFPKVKLSE
                     SVFINCEFINCDLTTCNISASVFRDAKFHTCNLTGLQFDSCNQLLLSFEFTGCKLNLA
                     SFFGMKLLKTEFTKCEMVEVDFGRADLSGSAFYDCDLKNSIFDSSNLSKCDFRKAINY
                     AIDPDNNKISKAKFSLIGIPGLLTKYDILIE"
     gene            complement(743..4009)
                     /locus_tag="VF870_09085"
     CDS             complement(743..4009)
                     /locus_tag="VF870_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9252384.1"
                     /translation="MHKLFTIFIATFLIITSVIIFGQNNSQSDSLKNISINGLSFRSV
                     GPAITGGRIVDIAVNLVNNSEYYVASGHGSLWKTTNSGVTFSPVFDGNKSYAIGCVVI
                     DPTNPNVVWVGTGENNNQSNVIFGDGIYKSEDAGKSWNNMGISASHQIGGIAIDPNNS
                     NIVYAAAYGSSRFSGGDRGIFKTTDGGKTWNNVLKISEFTGCYQIHMDPRYSNILYAV
                     AHQRMRNLYTGVYGGPESGIYRSLDYGATWEKLKNGLPQEDVGRIGMAISPVNPDYIY
                     AIVEATDKDKGVYKSTDRGASWTKQNSYNATAPFYYHELYCDPLELERVYSNDTFLQF
                     TVDGGKTWKNLGDDKKHVDNHALWIDPLNNKHILAGCDGGLYETFDRAKNWDFKPNLP
                     IAEIYKITTDNAEPFYNVYIGTQDNNSLGGPSRTISSAGILNQDWLFTNAGDGFETQV
                     DWKNPDIIYSQSQFGGLVRFDKRSGENLFIQPQDFADTAYRFDWDAGFLLSKHNNKRL
                     YIGGNKLLRSDDQGSTWKEISPDLTLGVPKEMQKLMGQSWSIDQLARKSSMAQIVTIG
                     ESPLDENILFVGSGDGLINFTTNGGINWTKAIIEGLPEYSRIHHIIASNFDKLVAYAA
                     CHNYLGGDYKPYIYKTTDGGKHWFSINNNLPEIGSTFSVAEDHINKNLLFLGTLFGVY
                     FSNDGGKEWIPLKNGMPPTCVMDLDIQRRESDLVVSTFGRGVYILDDYSPLRYLSTET
                     LKKEAEIFPVKDALMFIPSNPLGFKGVAFMGASFYSAPNPEVGAVFTYYLKDEFKTLK
                     DLRRDEEKKLQKKNSDIKYPAYETLKAEQEEPEAYLLFTITDEAGNTIRKIKTDANKG
                     INRVTWNFRYTPVTPISLEIPDNTIPWNEPDKGYMVLPGRYFVSLSKFKDGKFTELVS
                     KKEFVCKSLNNTTLPAEDLFALNTFNKKVAELTRSIAGADAFRNELVSKVAYLKKAVL
                     DGANVPTAAYNNIINIESDLRDLNLKLNGDQLRSRYEGSAPTSVKQRVDLITGALWNT
                     TAAPTNTFIKSYETAASKFDDILQSLKTIDTSVKQVESELEKYGAPFTPGRLPEWKKD
                     "
     gene            4583..>4939
                     /locus_tag="VF870_09090"
     CDS             4583..>4939
                     /locus_tag="VF870_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9252385.1"
                     /translation="MKIGFIGLGKMGFNMVQRLINDGHEVIVWNRDPEPIIEIEKKGA
                     ITAASIDDLVNKLPERKVVWLMVPSGNPVDENIDSLQKLLKEDDIIIDGGNSYWKDTQ
                     KRSEKLAKYRIHYLDCG"
BASE COUNT         1555 a          898 c          806 g         1680 t
ORIGIN      
        1 caataaaaaa acctggtcta tctcccggtt ttacaaaata tttcatgtat ttattttatt
       61 aggaacattt tcttatttct ggcttatgta taattatata agcttattta ggtaatgaga
      121 taggcattat acgattaata aaatttaaat tatattgaag tggttatgtc agatttagaa
      181 tactttacag ataaagaatt ttcgaacatc aatttttcaa agagcagatt tacgcctggt
      241 gaatatgaaa attgcaaatt tgagaaatgc gccttcccta aagttaaatt atccgaatca
      301 gtatttataa attgtgaatt tataaattgt gatttaacaa cctgcaatat ttctgcttca
      361 gtttttcggg atgcaaaatt ccacacatgt aatttaacag gtttgcaatt tgatagctgc
      421 aatcaattac ttttatcatt tgaattcaca ggctgtaaat taaacctggc ttcgttcttc
      481 ggaatgaaat tgctaaaaac agaattcaca aaatgcgaaa tggtagaagt cgattttggc
      541 agagcagatc ttagcggctc tgccttttat gattgtgatt tgaaaaactc aatatttgat
      601 agctcaaatt tatctaaatg tgattttcgt aaagcaatta actatgcaat agacccggat
      661 aataacaaaa tctcaaaagc gaaattttct ctgattggta taccgggtct attaacaaag
      721 tacgatattt taattgagta aattaatcct ttttccactc gggcaatctg cccggggtaa
      781 agggcgcacc gtatttctca agttcagatt cgacttgctt tacacttgta tctattgtct
      841 ttaacgactg aagaatatca tcaaatttac ttgctgccgt ttcataagat ttgataaaag
      901 tattggttgg tgccgcagtt gtattccaca aagcaccggt tattaaatcc acacgttgct
      961 ttacagaagt tggtgcggat ccttcatatc tgcttcttag ttgatctcca tttagtttta
     1021 aattaagatc tcttaaatca ctttcaatat tgataatgtt attataagct gcagttggaa
     1081 catttgcgcc gtctaatacc gccttcttaa ggtaagccac tttacttacc aactcgtttc
     1141 tgaatgcatc agcaccggca attgatcttg taagctccgc cactttttta ttgaatgtat
     1201 tgagcgcaaa caaatcttct gccggaagcg ttgtgttgtt taaagattta caaacaaatt
     1261 cttttttgct taccagttcg gtaaactttc catctttgaa ttttgagaga gacacaaaat
     1321 atctccctgg caaaaccatg taacctttat caggctcatt ccatggtatg gtattatcgg
     1381 gtatctcgag cgatattggt gtaacaggag tgtatctgaa attccaggtt actctattta
     1441 tccctttatt tgcgtctgtt tttattttgc gtattgtatt accagcttca tctgttatag
     1501 taaataatag ataagcttcc ggctcttcct gttcagcttt taaagtttca taggccggat
     1561 atttaatatc actgtttttc ttctgcaatt tcttctcttc atctcttcgc aagtctttta
     1621 atgttttgaa ctcatctttt aagtaataag taaatactgc acccacttcc ggatttggcg
     1681 ccgaataaaa acttgcaccc ataaatgcaa ctcccttaaa tccaagcgga ttagatggaa
     1741 tgaacattaa tgcatctttt acaggaaata tttcagcctc tttttttaat gtttctgttg
     1801 ataaatatct taaaggtgaa taatcatcaa gaatataaac tcctcttcca aatgttgaaa
     1861 caacaagatc actttctctt ctttgaatat caaggtccat tacacatgta ggcggcatac
     1921 cgtttttcaa tggtatccat tcttttccac catcattaga aaaatataca ccaaacaaag
     1981 ttccaagaaa taaaagattt ttattaatat gatcttcagc tacagaaaaa gtactgccaa
     2041 tttccggaag attattatta attgaaaacc aatgtttacc gccatcggtt gttttgtaga
     2101 tatatggttt gtaatcgccg cctaagtagt tgtggcatgc cgcgtacgca acaagtttat
     2161 caaaattaga agctatgatg tgatgaattc gtgaatattc gggcaatcct tcaattattg
     2221 cctttgtcca attaatgccg ccgtttgttg taaagtttat tagtccatca cctgaaccca
     2281 cgaataaaat attttcatcc agtggggatt ctcctattgt tacaatttgt gccatactac
     2341 ttttcctggc aagctgatca atactccagc tttgtcccat aagtttttgc atctctttgg
     2401 gaacacccag tgttagatcc ggactaattt ctttccaagt acttccctga tcgtcacttc
     2461 gtaataattt gttgccacca atgtataatc ttttattatt gtgttttgaa agcagaaagc
     2521 ctgcatccca atcaaatcga taagcagtat cagcaaaatc ctgcggctga atgaataaat
     2581 tttctccgct tcgtttatca aagcggacaa gaccgccaaa ttgggattgt gaataaatta
     2641 tgtccggatt tttccaatcg acttgcgtct caaaaccatc cccggcatta gtgaagagcc
     2701 aatcctgatt taatattccc gctgagctta tagttcgcga aggtcctcca agactgttgt
     2761 tatcctgtgt cccgatataa acattataaa atggttcagc attatcggtt gtaattttat
     2821 aaatttctgc aataggcaga tttggtttga aatcccagtt ctttgcccga tcgaaagttt
     2881 cgtaaagtcc gccatcacat ccggcaagaa tatgtttatt gtttaaagga tcaatccata
     2941 atgcgtggtt atcaacatgc tttttatcat cacctaaatt tttccaggtt tttccgccat
     3001 caacagtaaa ttgcaaaaaa gtatcgttgc tgtaaactct ttctaattct aatggatcac
     3061 agtaaagttc atgatagtaa aaaggtgcgg ttgcattata actattttgc ttagtccagc
     3121 ttgcgccgcg gtcagtgctt ttataaacac ctttgtcttt atcggttgct tcaacaatgg
     3181 catagatata atcaggatta acaggtgaaa tagccatgcc aattcttcca acatcttcct
     3241 gaggcaatcc atttttaagt ttttcccagg tggctccata atcaaggctg cgataaattc
     3301 cactttcggg tccgccataa acacctgtgt ataaatttct cattctctga tgtgcaacag
     3361 catataaaat gtttgagtaa cgcgggtcca tatgaatctg gtaacagcct gtgaattcgc
     3421 tgatctttaa aacattattc caggttttac ctccgtctgt tgttttaaaa attcctctat
     3481 caccgcctga aaatctggat gatccataag ccgctgcgta aactatgtta gaattatttg
     3541 ggtcaattgc aattccgcca atctgatgcg aagcgctgat tcccatgtta ttccagcttt
     3601 ttcccgcatc ctcacttttg taaattccat caccaaaaat cacattgctc tgattattat
     3661 tttcaccggt tcctacccaa acaacattag gattagttgg atcaataaca acacatccta
     3721 ttgcataaga tttattacca tcaaaaactg gtgaaaaggt tacgccacta ttagttgttt
     3781 tccacaaaga cccatgaccc gatgcaacat aatactcaga attattaacc agattaactg
     3841 cgatatcaac aattcttcct ccggtaattg ccggaccaac gcttctgaat gaaagtccat
     3901 taatggaaat atttttaagc gaatcggatt gtgaattatt ctgcccgaaa ataatcactg
     3961 aagttatgat taaaaaagta gcaataaaaa tggtaaataa tttatgcatg gaaatcaccc
     4021 gaaaataatt attgaaaatt tgatttggaa aacatttttt tacaaacact attttttctg
     4081 gaaaggatag atcaatatta aatagtttta aattctttaa taaaataata gcagtaaata
     4141 ataaaataca atattagcag gagataatta tgattagcgg tcatcataaa tgattaaaga
     4201 ataaaaacct gctgaaaggt tttttagctg attataagca ttctcatact gcattcagta
     4261 ctactcaaaa aatattttaa atatttttaa cagcatctat ataatgtttg attgctattc
     4321 tccggataaa ttttttttga tcaaaaaaat aagagaaagc tttcgtctgg ctttatcgac
     4381 tttattaacc gttgacagat gtgattcaga acaatataat tatgttgaga ttcagcgcaa
     4441 ttagaatgca tgcttcgtat catatttttt atcgtcgcat ttgtaaaact atttacatta
     4501 tttttcttac attaggatcg gatctagtga atcaaactga ttataaaatt tatattttag
     4561 cataaaacaa ttggagaaaa aaatgaaaat tggatttatt ggacttggca agatgggttt
     4621 taatatggtt cagcgtttaa tcaatgatgg acacgaggta atcgtttgga acagagaccc
     4681 agagccaata atagagatag aaaaaaaagg agcaattact gccgcatcaa ttgatgattt
     4741 agttaataaa ctacccgaaa gaaaagttgt ttggttgatg gttccttctg gaaatccagt
     4801 tgatgaaaat attgactctc ttcaaaaact attaaaagaa gacgatataa ttattgatgg
     4861 tggaaattcg tattggaaag acacacaaaa gcgttcagaa aaattagcta agtatcgaat
     4921 tcattactta gactgcggt
//
LOCUS       DASWBY010000507         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_55667, whole genome shotgun sequence.
ACCESSION   DASWBY010000507 DASWBY010000000
VERSION     DASWBY010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55667"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VF870_09095"
     CDS             <1..185
                     /locus_tag="VF870_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX9252386.1"
                     /translation="EEKKVIEKYDVWKEKSMYGRKYMGVERTTFIIDEKGKIKKIFNK
                     VKVDGHNKEVLEALKD"
     gene            187..594
                     /locus_tag="VF870_09100"
     CDS             187..594
                     /locus_tag="VF870_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX9252387.1"
                     /translation="MIYLTRRETFAAAHRLFREGLSEKENYELFGKCSNPNWHGHNYT
                     LEVVVAGNVDPATGFVIDLKKLKDIIHEHVISKVDHKNLNVDTDFMKGLNPTSENIVV
                     AVWNQLKDKIPAGKLYSVKLYETENNYFEYRGE"
     gene            596..1162
                     /gene="folE"
                     /locus_tag="VF870_09105"
     CDS             596..1162
                     /gene="folE"
                     /locus_tag="VF870_09105"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX9252388.1"
                     /translation="MDKDRTKNLINELLIQIGEDPNREGLLTTPKRVAEAYEFLTKGY
                     KQDIEEVINDAIFTEKYDEMVLVKNIDFYSMCEHHMLPFYGKVHVAYIPNGKIVGLSK
                     IPRIVDVFARRLQVQERMTRQIADTLHKYLQPIGVAVVSEAYHMCMMMRGVEKQNSSA
                     TSSAMLGVFKEDARTRSEFLNLISTKNL"
     gene            1159..1872
                     /locus_tag="VF870_09110"
     CDS             1159..1872
                     /locus_tag="VF870_09110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9252389.1"
                     /translation="MSKEKSGIWITGASSGIGRAAAIEFAKTGAKVFASGRRAVELER
                     INSEFENDKISVEVFPCNVASQTNVDQTVKKILANSKIDCLINNAGITSFKLAEENSI
                     NEINDIINTNLLGSIYAIKAVLPQMISNGGGTIINILSVVTKKTFTKSSAYAASKMGL
                     LGYTNSLREEVRKYNIKVINVIPGATATTMWSKEMRNQFSDRMMTSEDIARVLVWAYL
                     QKENLVTEELLLRPIEGDL"
     gene            complement(1905..2363)
                     /locus_tag="VF870_09115"
     CDS             complement(1905..2363)
                     /locus_tag="VF870_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX9252390.1"
                     /translation="MKNLILAFTMLFAITIVSPELYSQNKDHQRGKDLRMEIKKQLNL
                     TEDQEKKIETMRFSHQESMIKLKSDLELKELEMRKLKSSDNFSRSSIINLTKEISSIK
                     GDMALAVTNHQMDIYDLLDVNQKKIWLEMQDQFGNMKHRMRDKMRERENW"
     gene            2494..3045
                     /locus_tag="VF870_09120"
     CDS             2494..3045
                     /locus_tag="VF870_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9252391.1"
                     /translation="MHEINNDFDLIKGFINGDESAFNRLAVKYQEKIYWHARRMSGNH
                     LDADEIVQEVLIVLYKKLKTFEFKSSFYTWVFTITNTRSINHLKKRNLKNFFSLDDIS
                     NKNFERKDVIDDLESKQKIEKIESALQKLPVKQREVFIMRNFDELSYEEISRITGKSI
                     GALKANHFHALNKIKELVNYDKL"
     gene            3032..3649
                     /locus_tag="VF870_09125"
     CDS             3032..3649
                     /locus_tag="VF870_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252392.1"
                     /translation="MINYEEKFFAYLKGKMNSLEKNEFEDELNHSEILKNEFIEYKKL
                     NGIIDEIKNVQLNKDYSESIITEFRKRKENKKIKIISPTIKYALASIIIIVAGYFLMT
                     MTNKENLQEIKLNLEDFSEAELNSWNNNYYYSTSAFENSIDDDAVSKIDSIYKENLSE
                     SLVEALSEKKLEDFISINNVSDVDEYLSDNDVDMIYNQLINKEIL"
BASE COUNT         1341 a          501 c          658 g         1163 t
ORIGIN      
        1 acgaagaaaa aaaggttata gaaaagtacg atgtatggaa agaaaaaagc atgtacggca
       61 gaaaatatat gggtgttgaa agaacaactt ttatcatcga tgaaaaagga aaaatcaaaa
      121 agatatttaa taaagtaaag gttgatggac ataacaaaga agtgttggaa gctttaaagg
      181 attaatatga tttatcttac cagaagagaa acatttgctg cggctcatcg attattcaga
      241 gaaggtttaa gtgaaaaaga gaattatgag ttgtttggta aatgcagcaa tccaaactgg
      301 cacggacata attatactct ggaagttgta gttgccggaa atgttgatcc ggcaactggt
      361 tttgttattg atctgaaaaa actaaaagat attattcatg aacatgtaat aagtaaagtt
      421 gatcataaaa atctgaatgt tgatacagat tttatgaaag gattaaatcc gacatcggaa
      481 aatatcgttg ttgcggtctg gaatcagtta aaagataaaa ttcctgccgg caaactttac
      541 tcagttaaac tttacgaaac tgaaaataat tacttcgaat atcgcggaga ataaattgga
      601 taaagacaga acaaaaaatt tgattaacga attattaatt cagattggtg aagatccaaa
      661 tcgtgaagga ttactcacaa cgccaaagcg agtagctgag gcatatgaat ttcttactaa
      721 aggttacaag caggatattg aagaagttat taacgatgct atctttacag aaaaatatga
      781 cgagatggtt ttagttaaga acatagattt ttacagcatg tgtgaacatc atatgttgcc
      841 gttttatgga aaagttcatg ttgcatacat tccgaatgga aagattgtgg gattgagtaa
      901 gattccaaga attgtagatg tttttgcaag gcgattacaa gttcaggaaa gaatgacacg
      961 acaaattgcc gacactttac ataaatatct tcagccgatt ggtgtagcag ttgtatccga
     1021 ggcttatcat atgtgtatga tgatgcgtgg agttgagaag caaaactcat ctgcaacttc
     1081 gagcgcaatg cttggggtat ttaaagagga tgcaagaaca cgatcagaat ttcttaatct
     1141 tatttcaaca aaaaatttat gagtaaagaa aaatccggaa tctggataac cggtgcaagt
     1201 tcgggaattg gcagagctgc agcaattgag tttgcaaaaa caggtgccaa agtttttgct
     1261 tccggtcgaa gagctgtaga acttgaaaga attaattctg aatttgaaaa tgataaaatt
     1321 tcagttgaag tgtttccatg caacgttgca tctcaaacaa atgttgatca aactgtaaag
     1381 aaaattttag ctaattcaaa aatagattgt ttaattaata atgccggtat cacttctttc
     1441 aaattagccg aagaaaattc aattaatgaa ataaatgata tcattaacac aaatctttta
     1501 ggttcaattt acgcgataaa agctgtgctt cctcaaatga tttccaatgg cggaggaacg
     1561 ataattaata ttctttctgt ggttactaaa aaaactttta ccaaaagcag cgcttacgca
     1621 gcttcaaaga tgggcttgct tggctacaca aattcattaa gggaagaagt tagaaagtac
     1681 aacataaaag ttattaacgt tattccaggt gcaactgcaa caactatgtg gtccaaagaa
     1741 atgcgtaatc aattttctga tagaatgatg acaagtgaag atattgcacg tgttttagtt
     1801 tgggcttatc tgcagaaaga aaatcttgtt acagaggaac tcctcttaag acctattgaa
     1861 ggtgacttat aaaaaagccg gcttaaaccg gctttagatt tatgtcacca attttctcgt
     1921 tcccgcattt tatctctcat tctgtgtttc atattcccaa actgatcctg catttctaac
     1981 caaatctttt tttgattaac atctaataaa tcataaatat ccatttgatg atttgttact
     2041 gctaatgcca tatcgccttt aatagaactg atttcttttg tgagatttat aattgagctt
     2101 ctcgaaaagt tatcagatga tttgagttta cgcatttcca actctttaag ttcaagatcc
     2161 gattttaatt taatcataga ttcctgatga gagaatctca tagtttcaat ctttttttcc
     2221 tgatcttccg taagatttaa ctgtttttta atctccatcc ttaaatcctt ccctcgctga
     2281 tgatctttat tttgagagta aagttcgggg ctaactatcg ttattgcaaa cagcatggtg
     2341 aatgcaagaa ttaagttttt cataatttat acctttcgat gaatattttt aattttgatc
     2401 tttggacaac gaacaattac ttttggttta aaagaaatac aattaaacca tccgcagagt
     2461 tattctgtct aagtggtaac taaaaaaaac tgtatgcacg aaataaataa cgattttgat
     2521 ctaattaaag gctttataaa tggcgatgaa tcggctttta atcgacttgc agttaaatac
     2581 caggaaaaaa tatattggca cgcacgacgt atgtccggaa atcatcttga tgctgacgag
     2641 attgtacagg aagttttaat tgttctttat aaaaagctta aaacttttga atttaaatct
     2701 tctttttata cctgggtttt tacaattacg aacacacgca gcattaatca cttaaaaaaa
     2761 agaaatttaa agaatttttt tagcctggat gatatttcaa ataaaaactt tgagcggaag
     2821 gatgttattg atgatcttga atcaaaacaa aaaattgaaa agattgaaag tgcattacag
     2881 aaacttcctg ttaagcaaag agaagtattc attatgagaa attttgatga acttagttat
     2941 gaagagattt ccaggattac cggtaaaagc ataggcgcac ttaaagcaaa tcattttcac
     3001 gctttaaata aaattaagga actggttaac tatgataaat tatgaagaaa agttttttgc
     3061 ttatctaaag ggaaaaatga attccttaga aaaaaacgaa tttgaagatg agctaaatca
     3121 ttccgaaatt cttaaaaatg aatttattga atataaaaaa ctgaatggca ttattgatga
     3181 aataaagaat gttcagttaa ataaagatta ttctgagtcc attataaccg aatttaggaa
     3241 gcgaaaagag aataaaaaga ttaagatcat ttcaccaaca ataaaatatg cccttgcttc
     3301 aataataata attgttgctg ggtactttct gatgacaatg actaataaag aaaatctgca
     3361 ggaaattaaa ttaaatcttg aggatttttc agaggctgag cttaattcat ggaacaataa
     3421 ttattattat tctacttctg catttgaaaa cagcattgat gatgatgctg taagcaaaat
     3481 cgattcaatt tataaagaaa atctttctga aagtttagtc gaagctctaa gtgaaaaaaa
     3541 attggaagat tttattagta ttaataatgt ttctgatgtg gatgaatatc tttctgataa
     3601 tgatgtggat atgatttata atcaattaat taataaagag attttataag aggtaaatat
     3661 gta
//
LOCUS       DASWBY010000508         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_546668, whole genome shotgun sequence.
ACCESSION   DASWBY010000508 DASWBY010000000
VERSION     DASWBY010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546668"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VF870_09130"
     CDS             complement(<1..932)
                     /locus_tag="VF870_09130"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9252393.1"
                     /translation="MNSFEGLGLDQKILSAIKELNFENPMPVQEKIIPMMLQDEPQDI
                     IALAQTGTGKTAAFGLPIIHKTDTTLKQVQYLIIAPTRELCLQIADDLKDYAAHNKGI
                     KIAAIFGGSSMDRQIQNIKRGAQIISATPGRLNDLINRGEVNLSNVKAVVLDEADEML
                     NMGFKEELEAILETTPKTRNTFLFSATMPNPLLSIANRYMKKPVEVTIGRKNSGAENV
                     EHVSYLVHARDKYLALKRIVDFHPNVYGIVFCRTRQETKDVSEKLMQDGYDADALHGD
                     LSQSQREAVLNKFRTRHLRILVATDVAARGLDVD"
     gene            complement(1230..>2558)
                     /locus_tag="VF870_09135"
     CDS             complement(1230..>2558)
                     /locus_tag="VF870_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HEX9252394.1"
                     /translation="CSTPWRTIIVCDNAEDILASKLILNLNEPSKFEDVSWIKPKKYI
                     GIWWGMHIGTMSWNYADIGNIKLGEVDWDSLTPNGRHGATTENTKRYIDFAAKHGFDA
                     VLVEGWNVGWEDWFGAWKEEVFDFVTPYPDFDVVELESYAKSKNVELIMHHETSASVT
                     NYERRLDEAFKFMKEHGYNSVKTGYVGRIIPRGEHHDGQWMVNHFTRVVEKAEKYKIM
                     VNSHESVRLTGLHRTYPNWLAAEAARGNEFNAWSIGNPPEHETILPFTRFMGGPMDYT
                     PGIFQIKMDYYKPESKYQVHTTLAKQLALYVTIYSPIQMAADLPENYERFPDAFTFIE
                     QVPVDWSETKILKAEPGDYIVIARKDKNSENWFIGAITDEEARSFEISLDFLEAEKYE
                     MTTYKDAPEADWEKNPMKYEIEQSTVNKNQKIELKLAAGGGTAIVLKPIM"
BASE COUNT          747 a          519 c          409 g          883 t
ORIGIN      
        1 tcatcaacat caagaccgcg tgctgcaaca tcagtagcaa ctaatattct taagtgtctg
       61 gttctaaact tatttaaaac tgcttctctc tgtgattgcg atagatctcc gtgaagagca
      121 tccgcatcat aaccatcctg cataagtttt tcagaaacgt ctttagtttc ttgtcttgtt
      181 ctgcagaaaa ctattccata cacattagga tgaaaatcaa caattctctt taatgcaaga
      241 tatttatcgc gggcatgaac cagataactg acgtgctcaa cattttctgc tcccgagttt
      301 tttctgccaa ttgtgacttc aacgggtttt ttcatatatc ggttagcaat tgatagaaga
      361 ggattgggca tagtagcaga aaacaaaaat gtgtttctgg ttttgggagt tgtttctagt
      421 atcgcttcaa gctcttcctt aaatcccata ttaagcattt catcagcttc atcaagaaca
      481 acagctttaa cattacttaa attaacttcg ccacgattta ttaaatcatt caatctgcct
      541 ggagttgcag aaataatctg cgcaccgcgc ttaatattct gtatttgtct gtccatactc
      601 gaaccgccaa aaatcgcagc tatctttatt cctttgttat gcgctgcgta atcttttaag
      661 tcatcagcga tctgcagaca aagttcacgt gtcggtgcaa ttattaaata ctgaacctgt
      721 tttaatgttg tatctgtttt atgaataatt ggtaaaccaa aagcggctgt ttttcccgtt
      781 ccggtttgag ccaatgcaat aatatcctga ggctcatcct gcagcatcat tggaatgatt
      841 ttttcctgaa ccggcatggg attttcaaaa ttcagttctt taatagcact taatattttt
      901 tgatctaaac ctaacccttc aaacgaattc atttaaaagc tttctaaaaa tttttattta
      961 tttgattatt gattactctt tgtaagattt cactggctgg aatatcaact aaaatttctg
     1021 ttagtatatc gggcaagttc aaattctttt ttgatgattt gaatcgcaat taattaaatc
     1081 cgcaaacaag aagtaaatcg ttactgtaaa gataatgaaa attaaatgaa tcatagttat
     1141 atgaatcact tggaaaagtt cgattaaagt aaaaaccctt cctttttcct aaggaagggt
     1201 tttaatttga taaaatataa actataacat cacattattg gttttagaac tatagccgtt
     1261 cctccaccag ctgcaagttt taactcaatt ttctgatttt tatttactgt tgattgttca
     1321 atttcatatt tcatagggtt cttttcccaa tcagcctctg gtgcatcttt gtaagttgtc
     1381 atctcgtatt tttcagcttc cagaaaatct aaacttattt caaatgatct tgcttcttca
     1441 tcagtaatag caccaataaa ccaattctct gaatttttat ccttgcgagc gatgactata
     1501 taatctccgg gttcagcttt taatatttta gtttcactcc aatcaaccgg aacttgttca
     1561 ataaaagtaa atgcgtcagg gaatctctcg taattttcag gaagatcagc agccatttga
     1621 attggactgt aaatagttac atacaatgcg agctgttttg ccaaagttgt atgaacctgg
     1681 tatttgcttt ccggtttgta gtaatccatt ttaatttgaa aaattcccgg cgtgtaatcc
     1741 atcgggccgc ccataaaacg ggtgaaggga agtatagttt catgttcagg tggattgccg
     1801 atactccacg cattaaactc attacctctt gcagcttcag ccgcaagcca gttgggataa
     1861 gttctgtgca atcctgttaa gcgaactgat tcgtgcgagt tgaccatgat tttatacttt
     1921 tctgcttttt caactacacg agtaaaatga tttaccatcc attgcccatc atgatgttca
     1981 cctcttggaa tgattctgcc aacgtaaccc gtcttaacgg aattataacc atgttctttc
     2041 ataaacttga atgcttcatc taatcttcgc tcgtaattcg taacagatgc agaagtctcg
     2101 tgatgcataa tcaattctac attttttgac ttagcataac tttctaactc aacaacatca
     2161 aaatcgggat agggagttac aaaatcgaaa acttcttctt tccatgcacc aaaccaatct
     2221 tcccatccaa cattccagcc ttcaacaaga acagcatcaa aaccatgctt agctgcaaaa
     2281 tcaatatatc gttttgtgtt ttcagttgtg gcgccgtgcc tgccgttagg agtaagtgaa
     2341 tcccaatcaa cttctccaag ttttatatta ccgatgtctg cataattcca tgacattgtt
     2401 ccaatgtgca ttccccacca gatcccaata tattttttag gttttatcca gctgacatct
     2461 tcaaattttg atggttcatt taaatttaaa atcaattttg atgcaagaat atcttcagca
     2521 ttgtcgcata caataattgt tcgccaaggt gtgctgca
//
LOCUS       DASWBY010000509         5402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_735071, whole genome shotgun sequence.
ACCESSION   DASWBY010000509 DASWBY010000000
VERSION     DASWBY010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735071"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..1333
                     /locus_tag="VF870_09140"
     CDS             473..1333
                     /locus_tag="VF870_09140"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX9252395.1"
                     /translation="MYKIVEAQFIAPNIKRFEIEAPKIARKRKAGQFVIIRIKENGER
                     IPLTIADSNMQNGTITIIVQGIGKTTRELNTLLAADFILDVVGPLGKPSHIENFGTAI
                     SIGGGVGTAIAYPTAVALKEAGNYTISIIGGRSKEYVILEDEMKKVCDEVYPTTDDGS
                     YGFHGFVTTKLIELIKSEKKIDFVLAIGPIPMMKAVAETTRPYGIKTVVSLNPVMVDG
                     TGMCGGCRATVDNKTVFVCVDGPEFDAHLVDFETLSRRNSAYQQMEIISSELHKCNLD
                     KAFDKSFSNQ"
     gene            1363..2787
                     /gene="gltA"
                     /locus_tag="VF870_09145"
     CDS             1363..2787
                     /gene="gltA"
                     /locus_tag="VF870_09145"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX9252396.1"
                     /translation="MSELSKKDRMKIPRQPMPEQDPALRRSNFKEVNLGFTEELARME
                     ALRCLQCPKPVCIDGCPVGVKINEFIDLVSKGEFLAASAKIKDDNMLPAICGRVCPQE
                     EQCEAKCVVGKKGESIAIGRLERFVADFERDHAGIKAVELKPKTGRRVAVIGSGPAGL
                     SCAGDLIQMGHSVTVFEALHELGGVLIYGIPEFRLPKEIVKAEIESLKNLGVEFKTNA
                     VIGFTDTIDELLYAGFNAVFIAVGAGLPNFMNIEGENLNGVYSSNEFLTRVNLMKAYK
                     FPEYDTPVFNVKEKNVAVFGGGNTAMDAVRTSKRLGAKNAYIIYRRSETEMPARKEEV
                     HHAKEEGIEFILLSNPNRFIGDENGWLTGVEITKMELGEPDASGRRRPVPIPNSEYII
                     DIDMAVISIGNGSNPIIQKTTPDINFNKWGNIVVNEETMSTSKPGIFAGGDIVTGGAT
                     VILAMGAGRKAAKAINDYLKTLDK"
     gene            2801..4450
                     /locus_tag="VF870_09150"
     CDS             2801..4450
                     /locus_tag="VF870_09150"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEX9252397.1"
                     /translation="MAQYKESWGSRVGLVLAMAGNAVGLGNFLRFPAQASQNGGGAFI
                     IPYLISFFLMGIPLLYVEWAIGRHGGKFGHHSTPGMFDVLGKSRLLKYIGVFGLFTNL
                     TIAAYYCYIESWTLSYVFHSIIGTFTTVKPTEFFPKYLGITESSVFALPVEAFFWFVI
                     TLILNVWILSRGLAKGIELAAKIGMPLLILFGIILAIKGLTLDLSDPGVVQSPFVGLN
                     FVWEPNFSGLSNPTTWLAAAGQIFFTLSVGMGSIHCYASYVKEKQDIALNASSAGWMN
                     EFVEVILGGTILIPIATAYLGLSVVQASTAGGSGFALGFLTLPTLFNNWGWFAPFAGA
                     MWFGLLFFAGITSSLAMGQPIMAFMNDEYKISRHRSAVLFGIGTFILGFICVWLYPGG
                     SFDEFDFWTGTFSLVVFALLESIVFAFIFGIDKGWEEINRGSDIVIPKFFKFVIKYIT
                     PVFILIVFMGALFKPSIGWANAVYLLFTGNGWTFAKDSVIGVIFHIGEQKYTWFENGL
                     PTTILIKDGTRILLMVTFSVFTYLVHKAWKQKDINNNRGIK"
     gene            4701..5057
                     /locus_tag="VF870_09155"
     CDS             4701..5057
                     /locus_tag="VF870_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252398.1"
                     /translation="MKSTHNNKIKILSYLIAVLVLFMQVVPKTEFASAGEHRLPEWNI
                     DHNIALLNVSGNQPTKAAHRQIIELNLSPAHCFFDKYDILSIPELTDSKKIYGGGFSY
                     ANMYKTFLTVHYSTCT"
     gene            5123..5245
                     /locus_tag="VF870_09160"
     CDS             5123..5245
                     /locus_tag="VF870_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252399.1"
                     /translation="METEKKRSIMSDLPFYGLVGSLGLAILGIIVYMIWAIFVG"
BASE COUNT         1821 a          789 c         1105 g         1687 t
ORIGIN      
        1 gattttatca attgttcaat tagagatgga tttgtaagtg atacagttgc agcaaataag
       61 ttcattctta acaacaaggc tattaaaacg gagatcactt taaatcaact gaaagatagt
      121 ccgtacttag aattaaagat cgacacattg agaaaatagt aagtaatacg caggcactac
      181 aaaatattat aacttaacta ttgaaccttt cactataatt attctcaatt agagtaacta
      241 aataaaaaag gtttgattaa taattaacat tagctaaaaa attatagaga ataaaatgac
      301 tgagaataat gaaaaagaac gaagcttaaa agattttttc agggatctgg taatattatt
      361 gctccctttt gcaatattat atgcaatcta tgctttgttc ctcaaataat aaataaaaat
      421 taacaatatt aatttgctaa aaaatctctg atatttaatt gcaggattca aaatgtacaa
      481 gatagtagaa gctcaattca tcgcgccgaa tataaaaaga tttgaaatag aggctccaaa
      541 gattgcccga aagaggaaag ctggacaatt tgtaattatc agaatcaaag aaaatggcga
      601 aaggataccg cttaccattg ccgattctaa tatgcaaaat ggaactatta cgattatcgt
      661 tcaaggaatt gggaaaacaa caagagaatt aaacacgctt ttagcagctg attttattct
      721 ggatgtggta gggccacttg gtaagccatc gcatattgaa aattttggaa ctgcaattag
      781 tattggagga ggtgttggaa ctgcaatagc atatcccact gctgttgctt taaaagaagc
      841 gggaaattat acaatttcaa ttatcggcgg aagatcgaaa gaatacgtta tccttgaaga
      901 tgagatgaaa aaagtttgcg atgaagttta tccaaccaca gatgacggca gttatggctt
      961 ccacggattt gtgacaacta aacttattga gttgattaaa tcagaaaaaa agatcgattt
     1021 tgttttggcc atcggcccaa tcccaatgat gaaagccgtt gctgagacaa caagacctta
     1081 cggaatcaaa acggtagtca gtttaaatcc tgtgatggtt gacggaacag gaatgtgcgg
     1141 tggctgcaga gcaactgtag ataataaaac agtttttgtc tgtgtagatg gacctgaatt
     1201 tgatgcacac cttgttgatt ttgaaacatt atcaagaaga aattctgcgt atcaacaaat
     1261 ggaaatcatt tcatcagaac ttcacaaatg taatttggat aaggcatttg acaaatcatt
     1321 ttcaaaccaa taaaattttt tatctgtaac acaggagata gaatgagtga gcttagtaaa
     1381 aaagatagaa tgaaaatacc aagacaacct atgcctgaac aagatcctgc tttaagaaga
     1441 tcaaacttta aggaagttaa tcttgggttt acagaagaac ttgccagaat ggaagctctc
     1501 agatgtttgc agtgtcctaa acccgtttgc attgatggct gtcccgttgg agttaagata
     1561 aatgagttta ttgatttagt aagcaaagga gaattccttg cggcttctgc taaaattaaa
     1621 gatgacaata tgcttccggc aatttgcgga cgtgtctgtc cacaagagga acagtgcgag
     1681 gcaaaatgtg tcgttggtaa aaaaggagaa tcaatagcaa ttggaaggct tgaaagattt
     1741 gtagcagatt ttgaacgtga tcatgctggg ataaaggcag ttgaattaaa acctaaaaca
     1801 ggcaggaggg ttgctgttat tggaagcgga cctgcaggat taagttgtgc cggtgattta
     1861 attcaaatgg gacacagtgt gactgtattt gaagctcttc acgaacttgg cggtgtttta
     1921 atttatggta ttcctgaatt tcgtctgcca aaggaaattg taaaagctga aattgaatct
     1981 cttaaaaatc tcggtgtaga gtttaaaaca aatgccgtaa taggatttac tgatacaatt
     2041 gatgaattat tatacgcggg atttaatgct gtatttattg cagtcggagc aggactacca
     2101 aactttatga acatcgaggg agaaaatctt aatggggtgt attcatcaaa tgaattttta
     2161 acaagagtta atttgatgaa agcatataaa tttcctgaat atgatactcc tgtttttaac
     2221 gttaaagaga aaaacgttgc tgtctttggt ggaggtaata ctgccatgga tgcagtccga
     2281 acttcaaaaa gactcggagc aaaaaatgct tacataattt atagaagatc tgaaaccgaa
     2341 atgccagccc gaaaagaaga agtccatcac gcaaaagaag aaggaattga atttatttta
     2401 ctttctaatc ccaacagatt tattggagat gaaaatggct ggcttaccgg tgttgagatt
     2461 actaaaatgg aattgggtga accagacgca tcaggcagaa gaagaccggt tccaattcca
     2521 aattcagaat atataattga tattgatatg gcagtaattt caattggtaa tggttcaaac
     2581 ccgatcattc agaaaactac acctgatatt aatttcaata agtggggaaa tattgttgtt
     2641 aatgaagaaa cgatgtctac atccaagcca ggtatttttg caggagggga tattgttaca
     2701 gggggtgcta ctgttatcct tgcaatgggt gctggcagaa aagcagccaa ggctattaac
     2761 gattatttaa agacattaga caaataaaag taggattaat atggcgcagt ataaggaatc
     2821 ttggggaagt agagtcggat tggtcttagc aatggcgggc aatgctgtgg gtcttggaaa
     2881 ctttttaaga tttcctgctc aagcatcaca aaatggtggc ggcgctttta ttataccata
     2941 tcttatttct tttttcctga tgggaattcc cttgctgtat gtcgaatggg caattggaag
     3001 gcacggagga aagttcggtc atcattcaac acctggaatg tttgatgttt taggaaaatc
     3061 ccgattattg aaatacatag gcgtattcgg attattcact aatcttacta ttgccgctta
     3121 ctactgttac attgaatcct ggacactctc gtatgttttt cattctatta tcggaacttt
     3181 tacaacagta aagccaactg aattttttcc aaaatatctg gggataaccg agtcatcagt
     3241 atttgcatta cctgtggaag cttttttctg gtttgttata actttgattc taaatgtatg
     3301 gatcctttcc agaggattag caaaaggcat tgagttagct gcaaaaatag gaatgccatt
     3361 gttaatacta ttcggcataa tactggcgat taaaggatta acacttgatt tgagtgatcc
     3421 cggagtggtt caaagcccat ttgttggatt aaactttgtg tgggaaccaa atttttccgg
     3481 actttcaaat cccacaacct ggctggcagc agcaggacaa atctttttta ctttatccgt
     3541 tggaatgggt tcaatacatt gttatgcatc ttatgtaaaa gaaaaacagg atatagcact
     3601 taatgcatct tcagcgggct ggatgaatga atttgttgag gtgatattgg gtggaacgat
     3661 tttaatacct atagcaacag cataccttgg attatcagta gtacaggcat caacggccgg
     3721 cggcagtgga tttgcattag gatttttaac gctgccaaca ttatttaata attggggctg
     3781 gtttgcacca tttgccggag cgatgtggtt tggactttta tttttcgctg gaataacttc
     3841 ctccttagca atgggccaac cgataatggc atttatgaat gatgaatata aaatatcaag
     3901 gcatcggagt gccgtactat ttggtattgg cacatttata ttagggttta tctgtgtctg
     3961 gttatatccg ggtggatcat ttgatgaatt tgatttctgg acaggaactt tttcacttgt
     4021 agtatttgct ttattagaat cgatagtatt tgcttttatt tttggtattg ataaaggttg
     4081 ggaagaaatt aatcgaggtt ctgatattgt tattccaaag ttctttaagt tcgtaataaa
     4141 atatattaca ccagttttta tattaatagt ttttatgggt gcattgttta agccgtcaat
     4201 aggttgggca aatgcggttt atctcttatt tactggtaat ggatggacat tcgcaaagga
     4261 tagtgtaata ggagtaattt ttcatatcgg tgaacaaaaa tatacctggt ttgaaaatgg
     4321 gttaccaact acgattttaa taaaagatgg aacaaggatt ctgttgatgg tgacattttc
     4381 agtatttacc tacttagtac ataaagcgtg gaagcaaaaa gatattaata ataatcgagg
     4441 tataaaatga cagttgaagc aattataatg atggcagctg cctggactgt tataatattt
     4501 tttacggtaa aattttttat taaagtctta aaaattccac agaaagaaga ataaatgttt
     4561 agattatgat acacagggtt gtgctaacag atagttgaat ttataaaaca aaataaaata
     4621 aattaaaata atcagaaaac aagagttaag cgcttaatat gattgaaaat taaatttgct
     4681 aaagattaaa taaatatttg atgaaaagta cacataataa taaaataaag attttaagtt
     4741 acctaatcgc tgtattagta ttatttatgc aggtagttcc aaaaacggaa ttcgcaagtg
     4801 ctggtgagca tagattacct gaatggaata ttgatcacaa tattgcatta ttaaatgtaa
     4861 gcggcaatca acctacaaaa gctgcacatc gacagatcat tgaattaaat ttatctcctg
     4921 ctcattgttt ttttgataaa tatgatattt tatccatacc ggaattaact gattcaaaaa
     4981 agatatatgg cgggggattt tcatacgcaa atatgtataa aacatttttg actgtgcatt
     5041 attctacctg tacatagcga ttccaaacaa taatttctta gaattaacat taatcataaa
     5101 aaaatattaa aggaattggt atatggaaac ggaaaaaaaa agaagcataa tgagtgatct
     5161 ccctttttat ggtctagttg gatctttagg tcttgcaata ttgggtatca tagtctatat
     5221 gatctgggca atatttgttg gataaaaaat atgtataagg gatgaattat aaggaccctg
     5281 aagctttagc ctgcggctca gcaggtcggt ttcagggttc tttttttatg cagctggagg
     5341 tagatcatct gggcttctgc cgcaatcacc tttaccagta aaatttattg gacgtttatt
     5401 ct
//
LOCUS       DASWBY010000510         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_23593, whole genome shotgun sequence.
ACCESSION   DASWBY010000510 DASWBY010000000
VERSION     DASWBY010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23593"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VF870_09165"
     CDS             <1..558
                     /locus_tag="VF870_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA methyl transferase PRC-barrel
                     domain-containing protein"
                     /protein_id="HEX9252400.1"
                     /translation="IGSYKKKQIRELAHNYDLPNMTRKDSQGICFLGKIKFSDFIKHH
                     LGEVEGEIIDIDTNKVMGKHNGFYFYTIGQRSGLRLGGGPWYVISKDIEKNVVYISKE
                     NITNRARKEFTVGKFNWIADEKPEDGNYLVKIRHGEHFYKCSLNFDDYTAKVVTEKED
                     RGIAPGQFAVFYKDDVCLGGSIILE"
     gene            complement(867..1910)
                     /locus_tag="VF870_09170"
     CDS             complement(867..1910)
                     /locus_tag="VF870_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX9252401.1"
                     /translation="MRTRNLISTISLLISFFVFKGCSFTTYEKVYPTLGDGKYDSEFP
                     YKSTSEELNKISETIQRVNSTAFYKIYLFDEKNNFTLNDLKNKKLSDAAVKEALADNS
                     ASGTAVTVHSQNGNVALLTCAHTITFPDTIIAYRYDDLGFATQFIESISIKEKQVIYV
                     AGFPEGSQVDILAIDNQSDIALIGRKYGSQKGIFFPTFNYPLGKAKEVEWGTFVYLVG
                     YPINFKIITKAIVSSPRRDDAGSFLVDAVVNPGFSGGFVLAIRDGVPNFELIGMVQWV
                     PEEEESLIYPENPKTKSKYNPVVPYEGNLYVRKHSSIRYGIAKIIPVENIMTFLLENK
                     SYLNKQGFYIFQE"
     gene            complement(1955..3382)
                     /locus_tag="VF870_09175"
     CDS             complement(1955..3382)
                     /locus_tag="VF870_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9252402.1"
                     /translation="MSENSSPESTPEQNTIKSITKEITQPFIDLFHSSRALWGINLSY
                     LLEGITYFGVVGYLVIYFTDFVKLDDISAGQMVGFQTAGITLAMLFLGATVDWIGVRK
                     SLIYSLIFMLIGRIFLTAGPSITSNVGLWSSAHLIAMLGIFGIIIGYGIYQPACYAAV
                     KLFTTEKTSAMGYAMLYAIMNLGGFLPGRIAPPIRKAFATDEQGMLAVMWVYVGLTVL
                     GIAVVAIILTKKTIEKTIQEVSTSTKEQIEADRKAEDEKSAKEKIMFYIKNFPIRDLR
                     FMFFIFILIPVQTLFAHNWLTLPVYYKRAFTGVVSDNFEFFTNFNPLLIFILTPMVAA
                     LTVKRDTYKMMIYGTFVMAFPTFILALGPSIYTVFAYLILMTIGEAMWQPRFLQWVAE
                     IAPKGMTGIYMGIGQFPWFLTKVVTSLYSGWFLMHYAPEGVDPSKMNTETMWFIYGCI
                     AMVSSISLFFARGWMQKGFKTKHVE"
BASE COUNT         1229 a          603 c          593 g         1168 t
ORIGIN      
        1 atcggatctt ataagaaaaa gcaaattaga gaacttgctc ataattatga tttaccaaat
       61 atgaccagaa aagacagcca gggaatttgc tttcttggta agattaagtt ttctgatttt
      121 ataaaacatc atctcgggga agttgaaggt gaaataattg atatcgacac aaacaaggta
      181 atgggtaaac ataatggatt ttatttctat acaattggtc agcgttcagg tctaagattg
      241 ggcggcggtc cctggtatgt tatcagtaaa gatattgaaa aaaatgttgt ttacatctca
      301 aaagaaaata ttactaatcg tgccagaaaa gaatttacag tcggcaagtt taattggatt
      361 gccgatgaaa aacctgagga tggaaattat cttgtaaaga ttcgtcatgg tgagcatttc
      421 tataaatgca gtttaaattt tgatgattat acagcaaaag ttgtaacaga aaaagaagac
      481 agaggaatag ctccgggaca attcgcagtt ttttataagg atgatgtttg ccttggtgga
      541 agtattattt tagaataatt tccggataag taaagatcaa catacaatca ggcggtggtt
      601 gattgtgaaa aaaggatttg gtgaggattt gtagtatcca aatatttttc ttttgttatt
      661 ttaatcagat ttttacaatt cgtacagata taagcatatc taataccgat cacagaatcc
      721 aaccttgcaa cccataattc tgatatagtt ttattacaaa ttgggcaagt ggtattaatt
      781 tctatttgtt taatttcttt ggtcattctg aatctcggta tacataagac gctaaaaatg
      841 tttaaaaagt tactcaattt taaaaattat tcttgaaaaa tgtaaaatcc ttgtttgtta
      901 agatatgatt tattttcaag aagaaaagtc ataatatttt caacaggaat aatttttgcg
      961 ataccataac gaattgatga gtgttttctt acgtacaaat taccttcgta tggtactaca
     1021 ggattatact ttgattttgt tttaggattt tcaggataaa tcaaactttc ttcttcttca
     1081 ggcacccact gtaccatacc aattaattca aaattaggca caccatcacg aatcgcaagt
     1141 acaaatccgc cgctaaatcc cggattaact acagcatcaa caagaaaaga gcccgcatca
     1201 tctctgcgtg gactgcttac gattgctttt gtaataattt taaagtttat tggatatcca
     1261 acaagataaa cgaatgtacc ccattcaact tctttggctt tacctagtgg atagttaaac
     1321 gtagggaaga aaatcccttt ttgagatcca tattttctgc caattaatgc aatatcggat
     1381 tgattatcaa tagcaagaat atctacctgg ctgccttctg gaaatcctgc aacatatata
     1441 acttgttttt cttttataga aatcgattcg ataaattgtg ttgcaaatcc taaatcatca
     1501 tacctgtatg cgataattgt atcaggaaag gtaatagtgt gtgcacaggt taatagtgca
     1561 acattaccat tttgtgaatg aacagttact gcagttcctg aagctgaatt atctgcaaga
     1621 gcctccttaa cagcagcatc gctcagtttt ttatttttca gatcgtttaa tgtgaaatta
     1681 tttttttcat caaatagata aattttataa aacgcagttg aattaactct ttgtatagtt
     1741 tcactgattt tatttaattc ttcagaggtt gatttgtacg gaaattcact atcatatttt
     1801 ccatccccta atgtgggata aactttttcg tatgtagtaa atgaacatcc tttaaaaaca
     1861 aaaaaggata tcagtaaact gattgttgaa attaaatttc ttgttctcat tactaatatc
     1921 ctttaaatac ttttagataa aaccaataaa atttttattc aacgtgcttt gttttaaaac
     1981 ctttctgcat ccatccgcgg gcaaaaaata aacttatgct gctaaccatt gcaatacatc
     2041 cgtagataaa ccacattgtt tcagtattca tcttggaagg atcaacacct tcaggtgcgt
     2101 agtgcattaa aaaccagccg gagtataaac ttgtaacaac ctttgttaaa aaccacggaa
     2161 actgtcctat acccatataa attccagtca ttcctttagg tgcaatttca gcgacccatt
     2221 gtaaaaatct tggctgccac attgcttcgc ctatggtcat caaaataaga taggcaaaca
     2281 cagtgtaaat acttggacca agcgcaagta taaatgttgg gaatgccatt acgaaagttc
     2341 cataaatcat cattttatat gtatcccgct ttacagttaa agctgctacc atcggggtaa
     2401 gaatgaaaat taataatgga ttaaaatttg taaagaattc aaagttatca gaaacaactc
     2461 ctgtaaaagc tcttttataa taaactggaa gtgttaacca gttgtgtgca aataatgttt
     2521 gtacaggaat aagaataaag atgaaaaaca taaatcttaa atcgcgaatc ggaaagtttt
     2581 ttatatagaa cataattttt tcttttgcag atttttcatc ttctgctttt ctatcagctt
     2641 caatttgttc ctttgtgctt gtgctaactt cctgaattgt tttctcgata gtttttttag
     2701 tcagtattat tgcaacaaca gcaatgccca aaacagttaa tccaacataa acccacataa
     2761 cagctaacat tccttgctca tcagtggcaa aagcttttct tataggcggc gcaattcttc
     2821 cgggtaaaaa tccgccaaga ttcataattg catataacat tgcatagccc attgcagaag
     2881 ttttctcggt tgtaaatagt tttactgcag cgtaacaggc aggctgataa attccatatc
     2941 cgataatgat tccaaatatg ccaagcattg caataagatg tgcagaagac cataatccaa
     3001 cattagaagt tatgcttggt ccggcagtca gaaaaatcct gccgatcaac ataaagatta
     3061 aagaatagat aagagatttt ctaacaccaa tccaatcaac ggtggctccc aggaacaaca
     3121 tcgcaagagt tatacccgct gtttgaaatc caaccatttg tcctgcactt atatcatcaa
     3181 gttttacaaa atcagtaaaa taaataacta agtagccaac aactccaaag tatgtaatac
     3241 cttcaagcag gtacgaaaga tttattcccc atagagcacg tgatgaatgg aaaagatcta
     3301 taaaaggctg agttatttct ttagtgatgc ttttaatcgt gttttgttcc ggagtggatt
     3361 ctggtgatga attttcggac atgtttgttc ctgtaaaaaa tgattaataa ttatttagat
     3421 cttaaaaaat agttacaaac aaaaaagtat aaataaaaca acaaagcaaa gttataaagg
     3481 atacgctatt aaagcataag atataatcaa atcatttttc tttcttttaa tcctttcaga
     3541 ccttgtaacc cgccggtatg gattgcaaca atggtgctgc cggatggaaa aaa
//
LOCUS       DASWBY010000511         4081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_658191, whole genome shotgun sequence.
ACCESSION   DASWBY010000511 DASWBY010000000
VERSION     DASWBY010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658191"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2516
                     /locus_tag="VF870_09180"
     CDS             <1..2516
                     /locus_tag="VF870_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX9252403.1"
                     /translation="TGRGVHLVTVNDYLAKRDSEWMGEIFRFHGLTVGVILNTMDSAQ
                     RQQQYACDITYGTNNEFGFDYLRDNMSVDISQQVQRKHNYAIVDEVDSVLIDEARTPL
                     IISGPVDRDDHQFNDMKPRIERLYRLQKNFVANLVQQAEEMLNGEKNETEAGVLLFRA
                     QRGLPKNNKLAKMLSEPSYKRLVQSTEMEYLREKAKNMHIIDEELYFVIDEKSNQIDL
                     TEKGREELAKGSGFEKEYFILPDLGYEISKFENDETISLEEKVKRKDALYKKYSEASD
                     RIHTLNQLLKAYTLFEKDVEYVITEEGKIAIVDEFTGRVLPGRRYSDGLHQAIEAKEN
                     VKVERDSQTLATITLQNYFRMYNKLAGMTGTAETEEGEFYEIYKLEVVVIPTNRPIAR
                     DDQDDAVYKTKREKYNAIIEQIETLKREGRPVLVGTTSVEVSETLSRMLKRKGVQHNV
                     LNAKQHQKEAEVVQHAGELGAVTIATNMAGRGTDIKLGAGVVDKGGLYILGTERHESR
                     RIDRQLRGRAGRQGDPGTSKFYLSLEDDLMRLFGSDRISSVMERIGIKEGEVIQHPMI
                     SKSVERAQKKVEENNFAIRKRLLEYDNTMNSQREVIYTRRKKALQGDRLKSELLDLME
                     EMVDEIVEKNFEEVNVIAIREQVLQSLLVDIKLEPEEFETLGEAGVKKRIIDTAHDFY
                     KRKEEMLGTELMARLERYAVLSVLDHKWKEHLREMDDLKEGIGLRAYGQKDPLIEYKG
                     EAFKLFVELLNQIRNESVSFAFKFFPQAAEEVQRRRTAPIRMMESKQSTENIGLSNRP
                     PQTNPEGKVQTVRVENKIGRNDPCPCGSGKKYKQCHGKS"
     gene            2529..2867
                     /locus_tag="VF870_09185"
     CDS             2529..2867
                     /locus_tag="VF870_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9252404.1"
                     /translation="MKKIEAIIRPFKLDEVKEALVEEGIRGLTISEVRGYGRQKGHTE
                     TYRGSEYQIEFIPKIKIEIVVDDSLLDKVIEAILRTAKTGQVGDGKIFISDVKDVIRI
                     RTEESGSQAL"
     gene            2914..>4081
                     /locus_tag="VF870_09190"
     CDS             2914..>4081
                     /locus_tag="VF870_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9252405.1"
                     /translation="MIFRNKTGKLASIFVILLILSGCSVWNNFTTYFNLYYNTSTLFE
                     DAEKEILSQKRDLFSTEPLVIPGNSKTSLVKVVEKCSKILQFNSTSSYVDEALMMLGK
                     SFYYQGNYQKSKRKFEELLATDINDDQKITEASLWIAKNSFELREFTEALRLIKEVRV
                     KAVEEGYDDIIKDSYVQEIKYRIKEQDYPNAISLANEFADVYDDDVVRAQIYYELGKM
                     YTLIGENDNAILAYEKVFDNEPDFDLEIIATIKYAVALRNAGQTEKALELFEDIRTKD
                     KFLPSYNEIDFEIGKTLVQQGEYNRALDQFRMVDSTYKNTPFASASNFEMGDLYRTRF
                     LNYDSAGYYFSKAAISNPPKEYLDKAKSNNQLFLKYSKLRKEINKFDRQLYYTQN"
BASE COUNT         1508 a          587 c          847 g         1139 t
ORIGIN      
        1 taactggtcg cggtgtgcat cttgttactg ttaacgatta cttagctaaa cgcgatagtg
       61 agtggatggg agagatattt agatttcacg gtttaactgt cggtgtaata ttaaatacaa
      121 tggattctgc acagcgccag cagcaatacg cctgcgatat tacttacgga acaaacaacg
      181 aatttggttt cgattatctt cgtgataata tgtcggttga tatatcacaa caggtacagc
      241 gcaaacataa ctatgcaatt gtggatgagg ttgactccgt tttgattgat gaggcaagaa
      301 ctccgctcat catctccggt cctgttgata gagacgatca ccagtttaat gatatgaaac
      361 caaggataga aagactttat agacttcaga aaaattttgt tgctaatctt gttcagcagg
      421 ctgaagaaat gttgaatggt gaaaaaaatg aaactgaagc aggagtttta ttattccggg
      481 ctcagcgcgg attacctaaa aacaataaac ttgcaaaaat gctttctgaa ccatcatata
      541 aaagattagt tcaaagtacc gagatggagt atctaagaga aaaagccaag aatatgcaca
      601 tcattgacga agaactttac tttgtcattg atgaaaaaag taaccagatc gatcttactg
      661 aaaagggaag agaagaactt gcaaaaggca gcggctttga aaaagaatat tttattttgc
      721 ctgatcttgg atatgaaata agtaagtttg aaaatgatga aacaatatca cttgaagaaa
      781 aagtaaaaag aaaagatgca ctttataaaa agtattccga agcaagtgat agaattcata
      841 ccctaaatca gttattaaaa gcatatacac tttttgaaaa agatgttgaa tatgttataa
      901 ctgaagaagg aaagattgca attgtagatg aatttaccgg tcgtgtttta ccaggaagac
      961 gttacagcga tggcctacat caggcaattg aagcaaaaga aaatgttaag gttgaaagag
     1021 attctcaaac cctcgcaaca attacacttc agaattattt tagaatgtac aacaaacttg
     1081 caggaatgac cggaaccgca gaaacagaag aaggcgagtt ttatgaaata tacaaattgg
     1141 aagttgttgt tattcctaca aacagaccta ttgccaggga tgatcaggat gatgctgttt
     1201 acaagactaa acgtgaaaaa tataatgcaa taattgaaca gattgaaact ttgaagagag
     1261 agggaagacc tgtccttgtt ggaacaacct ctgttgaagt ttctgaaaca ctaagcagaa
     1321 tgctaaaacg aaagggtgtt cagcataatg ttcttaatgc aaaacaacat cagaaggaag
     1381 cagaagttgt acagcatgcc ggtgaattag gtgcagttac aatcgctaca aacatggcag
     1441 gtagaggtac tgatattaaa ctcggtgccg gtgttgtgga taaaggcgga ctttacatac
     1501 ttggaactga gcgacatgaa tcaagaagaa ttgacaggca gttaagaggt agagcaggaa
     1561 gacagggcga tcccggaaca tctaaatttt atctttcatt ggaagatgat ttgatgagac
     1621 tgttcggcag tgatagaatt tcttcagtga tggaaagaat cggaattaaa gaaggtgaag
     1681 taattcaaca cccaatgatt agtaaatctg ttgaacgcgc tcagaaaaaa gttgaagaga
     1741 ataactttgc tatcagaaaa agattacttg aatatgataa cactatgaat tctcaacggg
     1801 aagttatcta taccagaaga aaaaaagctt tgcagggtga tagattaaaa agtgaacttc
     1861 ttgatctaat ggaggaaatg gttgatgaaa ttgttgaaaa gaattttgaa gaagtaaatg
     1921 taattgcaat aagagaacaa gtgctgcaaa gtctacttgt ggatattaaa cttgaacctg
     1981 aagaatttga aacacttggc gaagcaggtg ttaaaaaacg aattattgat acggctcatg
     2041 atttctacaa gagaaaagaa gaaatgctcg gcacagagct gatggcaaga ttggaaagat
     2101 atgctgtgct tagcgtgctt gatcataaat ggaaagaaca tctgcgtgaa atggatgatc
     2161 ttaaagaagg tatagggtta cgagcctacg gacaaaaaga tcctctgatt gaatacaaag
     2221 gtgaagcatt taaattattt gttgaacttt taaatcagat aagaaacgaa tcagtttcat
     2281 ttgcgtttaa gttcttcccg caagcagcag aagaagttca aagaagaaga actgcaccaa
     2341 taagaatgat ggaaagcaaa cagagtactg aaaatattgg tctttcaaat cgtccgccgc
     2401 aaacaaatcc ggaaggaaaa gttcaaacag ttcgtgtaga aaataagatc ggaagaaatg
     2461 atccctgtcc ctgcggaagc ggaaaaaaat ataaacaatg ccatggcaaa tcataaccgg
     2521 agtcttctat gaaaaaaatt gaagcaataa tccgtccctt taaacttgat gaggtaaaag
     2581 aagctttggt tgaagaagga attagaggac taactatttc tgaagtacga ggatacggca
     2641 gacaaaaagg acatacagaa acttatcgcg gcagtgaata ccagattgag tttatcccta
     2701 agataaaaat agaaattgtt gttgatgatt ctttacttga taaagttata gaagccattt
     2761 tacgaacggc aaagacaggg caggttggag atggaaaaat attcatttct gatgtgaaag
     2821 atgtaattag aataagaact gaagaatctg gcagccaggc tttgtagaaa aaaaatgcct
     2881 ttttcttttt cttaaggttt aatagcttaa actatgattt ttagaaataa aaccggtaag
     2941 ctggcctcaa tatttgtaat tctgcttatt ttaagcgggt gctcggtctg gaataatttt
     3001 accacttact ttaatcttta ctataacact tcaactttat ttgaagatgc cgagaaagaa
     3061 atactttcac aaaaacggga tctgttttct actgagccat tagtaattcc gggtaattcc
     3121 aaaacttcac tcgttaaagt tgtagaaaaa tgttctaaga tattacaatt caattccaca
     3181 tcttcctatg ttgatgaagc attgatgatg ctgggtaaat ctttttatta tcagggaaat
     3241 tatcaaaaat cgaaaagaaa gtttgaagaa cttcttgcta ctgatattaa tgatgatcag
     3301 aaaattacag aagctagttt atggattgca aaaaattcat ttgaattaag agaatttact
     3361 gaggctctaa gattaattaa agaagttagg gttaaagctg ttgaagaagg ttatgatgac
     3421 attataaaag actcttatgt tcaggaaata aaatatcgaa ttaaagaaca ggattatcca
     3481 aatgccataa gcctggcaaa tgaatttgca gatgtttatg atgatgatgt ggttagagct
     3541 cagatttact atgagcttgg aaaaatgtac acgttaatcg gggaaaatga taatgcaata
     3601 ttagcatatg aaaaagtttt tgataacgaa ccagattttg atcttgagat aattgccacg
     3661 attaaatatg cagtagcatt gcgtaatgct ggacaaactg aaaaagcttt agaacttttt
     3721 gaagatatca ggactaaaga caaattttta ccttcgtata atgaaattga ttttgaaatc
     3781 gggaaaacac ttgtgcagca aggcgagtat aatcgggctc tggatcaatt tcgaatggtg
     3841 gattccactt ataaaaatac gccgtttgct tcagcatcaa attttgaaat gggtgacttg
     3901 tatagaacaa gatttttaaa ctatgatagt gccggatatt atttttctaa agctgcgatt
     3961 tcaaatcctc ccaaagaata tcttgataaa gcaaaaagta ataatcaatt gtttttaaaa
     4021 tattctaagc ttagaaaaga gattaataaa tttgataggc aactttacta cacacagaat
     4081 c
//
LOCUS       DASWBY010000512         7411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_55733, whole genome shotgun sequence.
ACCESSION   DASWBY010000512 DASWBY010000000
VERSION     DASWBY010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55733"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VF870_09195"
     CDS             <1..491
                     /locus_tag="VF870_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX9252406.1"
                     /translation="VIIAAKIFNLDNEKILAALKSFKGVEHRLELVREIEGIKFINDS
                     KATNIDSVIVALKSFDDPIFLILGGLDKGNDYSTIEELVIEKVKKIYAIGSSAEKIFN
                     YFHGKVKTEIKKDFDEIINTAISEAREGDVVLLSPACASFDMFDNYEHRGQIFKQIVN
                     SK"
     gene            488..1654
                     /locus_tag="VF870_09200"
     CDS             488..1654
                     /locus_tag="VF870_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEX9252407.1"
                     /translation="MKKLAITVFFASFSLILIGLIIVMSASNTYSAAKFDSSFYLFNS
                     HLSRVVFGILMMAVFSFIPYEWYKKISKPAVIGVAVLLFITLLFAPQVKGAGRWLNLG
                     FITIQPADIAKLILIIHLAYLIEKKRDDLLDFKKGFMFLFIWVLIIAGLIFIQPNVST
                     AALIVIISLTMLYVGGAKLKHIFLSSFSLIVAAGSFAMILPHSRKRILGFIATFGEGG
                     ELNHQLKQGMLSLGSGGLFGVGIGNSRQSNLFLPESYGDFIFAILGEETGFIGVVGVL
                     LFYVVLFVAGILIAKKAKDKFGQMLAFGISFSIIIYAFVNAAVATGLFPTTGLALPFI
                     SYGGTSIIFLCASIGILINIAIYNYQTEKNKDKQKQNDSEILDKTAFAFQKVKR"
     gene            1651..2754
                     /gene="murG"
                     /locus_tag="VF870_09205"
     CDS             1651..2754
                     /gene="murG"
                     /locus_tag="VF870_09205"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX9252408.1"
                     /translation="MKNVSTPYRFLFAGGGTGGHLYPAIAVADEIKRLKPESEIIFVG
                     TKTKIEGKVIPKLGYGFRAIWIKGFARKFNLENLLFPVKLFVSLIQSLFISFRFKPRV
                     AIGSGGYVAGPAIWGASVMGAKIILMESNSYPGVTTRLLERYADEVHISFEDSKKYLR
                     KPDKIKVTGNPVRSELGSSNKEDSKKSFGLDGSKFTVLVLGGSLGAASINQTVAACAE
                     ELQKKSLQIIWQTGKNYYHSYKSINLDSVKILDFIEDMNKAYSACDLLVARAGATTIA
                     ELSVLGIPSILIPSPHVAENHQYFNAKSLADNNAAILVKDSDVSSVLKERILEAAENK
                     ELLKSLSENSKKISRPDAANVIAKRAINFALAI"
     gene            2751..3230
                     /locus_tag="VF870_09210"
     CDS             2751..3230
                     /locus_tag="VF870_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252409.1"
                     /translation="MIDEQIKYKSKFKYNMSFYYQSTIIYFVAFAIYLLIRGQFVESS
                     YTLITHDPIIYLLAFIVVISVLTLLFNLYRNRYIEFSDDGLIFSDRFGSKLIHKNEIK
                     EIKLGKDRRFGKANPLKLVRIKIKNRIRPIIIRPNDYENESELLKRFHQLKLEVEKV"
     gene            3231..4598
                     /gene="murC"
                     /locus_tag="VF870_09215"
     CDS             3231..4598
                     /gene="murC"
                     /locus_tag="VF870_09215"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX9252410.1"
                     /translation="MFKNIKKVHFVGIGGIGMSGIAEILLSQGFIISGSDRSKSEVTD
                     RLESIGIKVYEGHSPENLKDADVLVYSSAVQIDNPEVKAAIDRKIPIIKRSEMLAETM
                     RMKYGIGIAGTHGKTTTTSMVGLTLTEGGIDPTIIVGGKLSGLGGTNARLGNGEFIVV
                     EADEFDRTFLKLTPTIAALTTLESEHLDTYKDLDDIKGAFIEFANKVPFYGFVTLCLD
                     EPALQDIMPHINKKIITYGLNAQADVRATDITHNGFASTYKVKYFGKELGEITLKIPG
                     NHNINNSLVAVVIGKELGVDFKVIKSALESFTGVFRRFEVKYNKEILVIDDYAHHPTE
                     VSATLKGVRDGWDRRLVVIFQPHLYSRTRDFYQEFGRAFLNSDVFICTAVYPARELPI
                     EGVTGDLIASTTKKIGHKNVHYVADKNDVPALLNQIKKDGDIVVTMGAGDIWKYGEKF
                     VAMLN"
     gene            4604..5371
                     /locus_tag="VF870_09220"
     CDS             4604..5371
                     /locus_tag="VF870_09220"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX9252411.1"
                     /translation="MKKDFGKIFGVVLLSVIVVSILYLSLFTYNKKGKEEIKMINIQG
                     NSLLSKEIYMRFADIDQPSVYDDLSLNLIKKRIEEHPYVAKAEVKSDGIGNVDVKIIE
                     KDIYAVLLGRSEPYFLTSDFELLKIHQFTTYSDIPVISNFRTSEKLAPSKVYKTEDIV
                     EAFKIIDAAKITDLELAKKLSEINLKNGGDVVLTFSGVSYPIVFGRGSESKKMIYLSL
                     VWERINTLKASYQNTEYIDLRFNNEAYIGSKENTGLI"
     gene            5371..6621
                     /gene="ftsA"
                     /locus_tag="VF870_09225"
     CDS             5371..6621
                     /gene="ftsA"
                     /locus_tag="VF870_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX9252412.1"
                     /translation="MKKDILVGLDLGTTKVCAVIAEKIPEQNKIDILGFGVAPSEGLH
                     KGLVANIGKTAEAIKSAMSIASNRAGFEVTTVNVGVAGEHITSIRHRNYVTINRDEKE
                     ITKDDLDRLEADVRTIRIPSDRQILHIIPEEFSIDHQTGIENPIGMSGSRLEASNHVV
                     LASIPAMENIRKSVERAGYQVQDYILQPIASSASVLEESEKDLGVALIDIGGGTTDIA
                     IYHKKAIKHTRVIGVAGNQVTNDIRESLGVITDEAERLKKEYGYASESAIIKDEDILI
                     KGVGARGNTKIPISLLTQIISLRMKELFTLVDNELRTAGFKSKIKAGIVLTGGGSLLR
                     GVTELAEEVFGLPTRIGVPQELGAGLSNEIESPEFATVAGLIRGIPGGKSSEYQVLIK
                     KQKDKSANQLGKIIKKVQDFFNEL"
     gene            6641..>7411
                     /gene="ftsZ"
                     /locus_tag="VF870_09230"
     CDS             6641..>7411
                     /gene="ftsZ"
                     /locus_tag="VF870_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9252413.1"
                     /translation="MSRFATIDKEKPMSAVLKVVGVGGGGCNAIESMIARGLSGVEYI
                     AVNTDSQVLRSSTATHKVQCGGAITRGLGAGADPNIGRKAAEEDRDQLAEVLSGSDMV
                     FITAGMGGGTGTGAAPVIASIAKSIGALVVGIVTKPFRWEGKARMMNAEKGIMELRQH
                     VDSLIVIPNERLLNILDKATNAFAAFDKPNEVLYEATRGIADIITIEGLINVDFADVR
                     AVMNSSGEALMGCGIASGENRAIEAAQKAISSPLLEGVN"
BASE COUNT         2529 a         1024 c         1512 g         2346 t
ORIGIN      
        1 cagtaataat tgctgctaag atatttaatc tggataatga gaaaatcctt gcggctttaa
       61 aatcatttaa aggtgttgaa cacagattag aattagttag agaaattgaa ggcataaagt
      121 ttataaatga ttctaaggcg acaaatattg attcagttat agttgcacta aaaagttttg
      181 atgatccgat atttttaatt ctcggtggtc tggataaggg aaatgattat tcaactattg
      241 aagaattagt tattgaaaaa gttaaaaaga tttatgctat tggttcatca gcggaaaaaa
      301 tctttaatta ctttcacggc aaagtaaaga cagaaatcaa aaaagatttt gatgaaataa
      361 taaacacagc aataagtgaa gcaagggaag gagatgtagt tttactttct ccggcttgtg
      421 caagctttga tatgtttgat aattatgagc atcgcggcca aatatttaag caaattgtaa
      481 atagtaaatg aaaaaattag caattacagt tttctttgca tcattcagtt taatccttat
      541 aggattaata attgtaatga gtgcgagcaa cacttatagc gctgcaaagt ttgatagctc
      601 attctatcta tttaactctc atctttcaag agttgtattt gggatattaa tgatggctgt
      661 ctttagtttt attccatacg aatggtataa aaaaattagc aagccagctg ttatcggtgt
      721 agcagtttta ttatttataa ccttgttgtt tgcaccacaa gttaaaggtg caggaagatg
      781 gttaaatctt ggtttcatta ccatacaacc tgcagatatt gcaaaattaa ttttgataat
      841 tcatctcgct tacttaattg aaaagaagag ggatgattta ctcgatttta aaaaaggttt
      901 tatgtttttg ttcatttggg ttttgataat tgcaggattg atttttatac agcctaatgt
      961 tagtacagcc gccctgatag taattatttc gttaacaatg ttgtatgtag gcggagcaaa
     1021 gttaaaacat atttttcttt catcatttag tttgattgta gccgctggat catttgcaat
     1081 gatattacct cactccagaa aaagaatttt aggattcatt gcaacctttg gtgaaggtgg
     1141 tgaattaaat catcaactaa aacaaggtat gctaagctta ggcagtggtg gattatttgg
     1201 tgttggaatt ggcaatagca gacaaagcaa tttgttttta ccagaatcct acggtgattt
     1261 tatatttgca atccttggtg aagaaacagg atttattggt gttgttgggg ttctactctt
     1321 ttatgtggtt ttatttgtag cgggaattct aatcgcaaag aaagcaaagg ataagtttgg
     1381 acaaatgctt gcatttggaa tatcattttc aataatcatt tatgcatttg taaatgccgc
     1441 tgttgctacc ggattatttc caacaacagg acttgcatta ccatttataa gctacggagg
     1501 aacatcaata atatttctat gcgcctctat tggcatatta ataaacattg cgatttataa
     1561 ttatcaaact gaaaaaaata aagacaaaca aaaacaaaat gattctgaaa tattagataa
     1621 aacagctttt gcttttcaga aagttaaaag atgaaaaatg tttcaactcc atatcgtttt
     1681 ctttttgctg gcggcggaac cggcggccat ctttatcctg caattgcggt tgcagatgag
     1741 attaaaagat tgaaacctga atcagaaata atttttgttg gtaccaaaac taaaatcgaa
     1801 ggtaaagtaa taccaaaact tggttatgga tttagagcta tctggataaa aggatttgca
     1861 cggaaattta atctggaaaa tttattgttt cctgttaaat tatttgtttc gcttattcaa
     1921 tcattgttta tctctttcag atttaaaccc agagtagcaa ttggttctgg tggatacgtt
     1981 gccggtcctg caatctgggg agcatctgta atgggagcaa aaattatttt gatggaatca
     2041 aatagttatc cgggtgttac aactcgatta ttagagcgtt atgcagatga agtacatatt
     2101 tcttttgaag attcgaaaaa gtatttgaga aaaccggata aaataaaagt aaccggtaat
     2161 cctgttcgtt ctgaactagg ttcatcaaat aaagaagatt ccaaaaaatc ttttgggctt
     2221 gatggaagca agtttacagt tcttgttctt ggcggaagtt taggtgctgc atcaattaac
     2281 cagacagttg cagcttgtgc agaagagttg caaaaaaaat ctttgcaaat catctggcaa
     2341 acaggaaaaa attattatca tagctataaa agcattaatt tagattccgt aaaaattctg
     2401 gattttattg aagacatgaa caaagcttac tcagcttgtg atcttcttgt ggcgagggcc
     2461 ggcgcaacca caattgctga gttatctgtt cttggaattc cttcaatact aattccatcg
     2521 ccccacgtcg cggagaatca tcaatatttt aatgctaaat cacttgcaga taataatgct
     2581 gcaatattgg ttaaggattc tgacgtaagt tcagttctta aagaacgaat tttggaagcg
     2641 gctgagaata aagaattact gaaaagctta agcgaaaatt caaaaaaaat ttctagacct
     2701 gatgcagcaa atgtaatcgc taagagagca ataaattttg cgttagcaat atgatagatg
     2761 aacaaataaa atataaaagt aagttcaagt ataatatgtc gttttattat caatcaacga
     2821 ttatatactt cgttgcattt gcaatttact tattgattcg tggtcagttt gttgaaagct
     2881 cttatacttt gattacccac gatcccataa tttatttgct ggctttcatt gttgttattt
     2941 ctgtgttaac attattattt aatctttatc gtaaccgata cattgagttc agtgacgacg
     3001 gattaatttt tagtgatcga tttggttcaa agcttattca taaaaatgaa attaaagaaa
     3061 ttaaacttgg taaagataga agatttggta aagctaaccc tttaaaactt gttaggataa
     3121 aaattaaaaa tagaattaga cctataatca ttagaccgaa tgattatgaa aatgaaagcg
     3181 aattgcttaa aagatttcat caacttaaac ttgaagtaga aaaagtataa atgtttaaga
     3241 atattaaaaa agttcacttt gttggaattg gcggaattgg aatgagcggc atcgctgaaa
     3301 ttcttttaag tcagggtttt atcatttccg gttcggatag atctaaaagt gaagttactg
     3361 atagactgga aagcatcgga ataaaagttt atgaaggaca ttcgccggaa aatttaaaag
     3421 atgcagatgt tcttgtttat tcatctgcag tgcagattga taatcctgaa gttaaggcag
     3481 cgattgatag aaaaattcct attataaaaa gatctgaaat gcttgctgaa acgatgcgaa
     3541 tgaaatatgg aattggtatt gcaggaacgc acggaaagac tacaacaaca tcaatggttg
     3601 gattaacttt aaccgaaggc ggaattgatc caacaataat agttggtgga aaacttagcg
     3661 gacttggcgg aactaatgca agattgggta atggagaatt tattgttgtt gaagcagatg
     3721 agtttgatag aacattttta aagttaactc caacaatagc agcattaaca actttggaaa
     3781 gcgagcatct tgatacttat aaagatcttg atgatatcaa aggagcattc attgagttcg
     3841 caaataaagt tccgttttat ggttttgtta ctttgtgttt agatgaacct gctttgcagg
     3901 atattatgcc gcatataaat aaaaagatta ttacttatgg attaaatgcc caggctgatg
     3961 ttcgtgcaac agatattact cataatggtt ttgcaagcac ttacaaagta aaatattttg
     4021 gaaaagagct tggtgagata accttaaaga ttcctggtaa tcacaacata aataattctc
     4081 ttgttgctgt tgtgattgga aaagaacttg gtgttgattt taaggttatt aaatctgcat
     4141 tagaatcctt tacaggtgtt tttagacgat ttgaagtaaa gtacaataaa gaaattttag
     4201 ttattgatga ttatgcgcat catccaacag aagtttcagc aactttaaaa ggagttagag
     4261 acggttggga tagaagattg gttgttattt ttcagcctca tctttattca cgcacaagag
     4321 atttttatca ggaatttggc agggcatttc ttaattctga tgtttttatt tgtactgctg
     4381 tttatccggc tagagaatta cctattgaag gtgtaaccgg agatttaatc gcatcaacaa
     4441 caaaaaaaat tggacataaa aatgttcatt atgttgccga taaaaatgat gttcctgcgt
     4501 tattaaatca gataaaaaaa gatggtgata tagttgttac gatgggtgct ggtgatatct
     4561 ggaaatatgg tgaaaagttt gttgcgatgt tgaattaaaa attatgaaaa aagattttgg
     4621 aaaaatattt ggtgtagttc ttttatcagt aattgtagtt tcgattttgt acttatctct
     4681 ttttacatat aacaaaaagg gaaaagagga aattaaaatg ataaacattc aaggaaacag
     4741 tttgcttagc aaagaaatct atatgcgttt tgctgatata gatcaaccat cagtctatga
     4801 tgatttaagt cttaacctga tcaaaaagag aattgaagaa catccatatg ttgcaaaagc
     4861 agaagtaaaa tctgatggaa tcggaaatgt tgatgtgaag attattgaaa aagatattta
     4921 cgctgtactg ctaggcagat ctgaaccata ttttttgact agtgattttg aattattaaa
     4981 gatccatcag tttacaactt actctgatat tcctgttatc agcaatttca gaacttcaga
     5041 aaaacttgca ccatcaaaag tttataaaac tgaagatatt gttgaagcat tcaagataat
     5101 tgatgctgct aaaattacag atctggaact tgcaaaaaag ctgtcagaga tcaatcttaa
     5161 aaacggtgga gatgttgtat taactttttc aggagtaagt tatccaattg tgttcggaag
     5221 aggcagtgaa tcaaaaaaaa tgatttatct aagtttagtt tgggaaagaa ttaatacttt
     5281 aaaagcaagt tatcagaata ctgaatacat cgatttaaga tttaataatg aagcttacat
     5341 tggaagcaaa gaaaacacag gcttgattta atgaaaaaag atattctagt tggtttggat
     5401 ttaggtacaa caaaagtttg cgcggtaatc gcagaaaaga ttcccgagca aaacaaaatt
     5461 gatatacttg gatttggcgt tgctccatcg gaaggactgc ataaaggttt agttgctaac
     5521 atcggtaaaa cagcagaagc aattaaatct gcaatgtcaa tagcttctaa cagagcagga
     5581 tttgaagtta caacagtaaa tgttggtgtt gccggagagc atattactag tattagacac
     5641 agaaattatg ttacaatcaa tcgtgatgaa aaagaaatta ccaaagatga tcttgataga
     5701 ttagaggcag acgttcgaac aataagaatt ccaagcgatc gacaaattct tcatataatt
     5761 cctgaagagt tttcaattga tcatcaaacc ggaattgaaa atcctattgg aatgtccgga
     5821 tcgagattag aggcaagtaa tcatgttgtg cttgcatcaa ttccggcaat ggaaaatatc
     5881 agaaaatctg ttgagcgtgc cggttaccag gttcaggatt atattttgca gccaatcgca
     5941 tcaagtgctt ctgtgttaga agaaagtgaa aaagatttag gtgtggcttt aatagatatt
     6001 ggcggaggta caactgacat tgccatctac cataaaaaag caattaagca taccagagtt
     6061 atcggagttg caggaaacca ggttacaaat gatattcgcg aatctttggg tgttattact
     6121 gatgaagccg aaagactaaa aaaagaatat ggctatgcaa gtgaaagtgc tatcattaaa
     6181 gatgaagata ttttgattaa aggtgttggc gcacgaggca atacaaaaat tccgatttca
     6241 ttgcttacac aaattataag tttaagaatg aaagagctct ttactcttgt tgataatgaa
     6301 ttgagaaccg caggatttaa atctaaaata aaagcgggaa ttgttttaac cggaggcggt
     6361 tcattattaa gaggtgttac agaattggca gaagaagttt ttggtttgcc aaccagaatt
     6421 ggtgttccgc aggaattggg tgcaggatta tcaaatgaaa ttgagagtcc tgaatttgca
     6481 actgtagctg gtttgataag aggaataccg ggagggaagt cgagtgagta tcaggttttg
     6541 attaaaaaac aaaaagataa atctgctaat caactcggaa agattataaa aaaagtgcaa
     6601 gattttttta acgaattata aactcattat aggaggacct atgtcgcgat ttgcaaccat
     6661 cgataaagaa aaaccgatgt cagcagtttt aaaagttgtt ggtgttggtg gcggaggctg
     6721 taatgccatc gaaagtatga ttgctcgcgg tttaagcggt gttgagtaca tcgctgtaaa
     6781 taccgattca caagttctaa gaagcagtac tgcaacacat aaagttcagt gtggtggtgc
     6841 tattacacgc ggcttaggtg ccggagctga tccgaacatc ggaagaaagg ctgcggagga
     6901 agatcgtgat caacttgctg aagttctttc aggtagtgat atggtgttta taactgctgg
     6961 tatgggcggt ggaacgggaa ctggcgctgc tcctgttatt gcatcgatcg caaaaagcat
     7021 aggtgcgttg gttgtaggta tcgttaccaa acctttccgc tgggaaggca aagcaagaat
     7081 gatgaatgct gaaaaaggta taatggaatt acgccagcac gttgatagtt taattgtaat
     7141 cccaaatgaa agattactta acattttaga taaagctaca aatgcttttg cagcttttga
     7201 taaacctaat gaagtacttt acgaagcaac acgcggtatt gctgatatca tcaccattga
     7261 aggcttgatt aatgttgatt ttgcagatgt tcgtgctgtg atgaattcca gcggtgaggc
     7321 tttaatggga tgcggaattg ccagtggtga aaatcgtgca attgaagctg ctcaaaaagc
     7381 aatttcaagt cctttacttg aaggtgtaaa t
//
LOCUS       DASWBY010000513         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_699033, whole genome shotgun sequence.
ACCESSION   DASWBY010000513 DASWBY010000000
VERSION     DASWBY010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699033"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VF870_09235"
     CDS             <1..1069
                     /locus_tag="VF870_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252414.1"
                     /translation="TTISSAFGPINAPCDIEVFPDTSIILIGDNGTGIFRSTDYGLTW
                     SQTYNTSGEIPTISVDHQNPGVAFATKWSGGGGMLKSTNFGQTWTNVIYFSSINMWGV
                     HIQESDGNIILANSYSTSPGSWRSVDAGVTWTPINIPSPGYQVVSIDTITQFAAQGGG
                     FYKLDSPFFIPVELTSFSASVVGGSTNLFWTTATELNNLGFDIEKSFDNKIFEKIGFV
                     PGYGTTTESKSYNFSASNVSSQKTFYRLKQLDFDGTFEYSSSVEVEGLTPTEFSLKQN
                     YPNPFNPSTKIGFTLPYESNVRISIYNLIGQKVSEVVNSKFNAGTHTVIFDASNFSSV
                     IYLYKIEAGNFTSVKKMQLMK"
     gene            1175..>2586
                     /locus_tag="VF870_09240"
     CDS             1175..>2586
                     /locus_tag="VF870_09240"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9252415.1"
                     /translation="MKNLLSALLSILFITSISAQTMKTAFINGKVYTVNEKQPLAQSV
                     IVEGNKILFVGSDEDAKKLFDASTKIIDLKGKLMLPGFIDNHVHFITGGFYLLGIDLR
                     PAASTNEFRKILKVYREKYPNKWITGGYWDHEKWEVKDLPTKEMIDDISPNTPVFVER
                     LDGHMGLANSYALKLAGITKDTESPDGGLIVKDSKTGEPTGILKDNAMNFIYNILPAA
                     TAEENYDAAVAALEEAKRLGITSVQDITFEDALNTFTKLKDEGKLTCRIFTRWPIADY
                     QSLVKKNIQVGYGDELIRMGSLKAFADGSLGSSTAWFFEKYNQDTTTFGLPMDIITNG
                     EMEKWCLDADKNHLQFSVHAIGDKANSWMLDLFEKITKENPKWDRRFRIEHAQHVRFE
                     DIKRFAQLGVIASVQPYHCIDDGVWAEKRIGPERIKYTYPFKSFLDAGVKMCFGTDWY
                     VAPLNPLLGIYAAVTRRTLD"
BASE COUNT          855 a          408 c          505 g          818 t
ORIGIN      
        1 gacaacaata tccagtgctt ttggacctat taatgctccc tgtgatatcg aagtttttcc
       61 tgatacttca attattctta ttggcgataa cggcacggga atttttagaa gtacagatta
      121 tggtttgacg tggtctcaaa cttacaatac atcgggtgaa attccgacaa tttcagttga
      181 ccatcaaaat ccgggtgttg cctttgccac aaaatggagt gggggcggtg gtatgttaaa
      241 gtcaacaaat tttggccaaa cctggacaaa tgtaatctat tttagttcta tcaatatgtg
      301 gggagtacat attcaagaat ctgatggaaa tattatttta gctaacagct attcaacttc
      361 acccggaagc tggagatctg ttgatgctgg tgtaacatgg acaccaataa acattccctc
      421 accgggttat caggttgttt cgattgatac tattacacag tttgcagcac aaggaggtgg
      481 attctataaa cttgattctc cattctttat ccctgttgaa cttacttcat tcagtgcatc
      541 agttgttggc ggcagcacaa atttattttg gactactgcc actgagctga ataatcttgg
      601 ctttgatatt gaaaagagtt ttgataataa aatttttgag aagattggat ttgttccggg
      661 gtatggaact acgacagaaa gtaaaagtta taatttctct gcatcgaatg tttcatcgca
      721 aaaaactttc tacaggttaa agcaattaga ttttgatggc acatttgaat actcatcatc
      781 tgttgaggtt gaaggattaa caccaactga attttcactt aaacagaatt atccaaatcc
      841 atttaatcca tccacaaaaa ttggatttac tctgccgtac gaaagtaacg ttagaataag
      901 tatatataat ttaataggac aaaaagtttc tgaggttgtt aattcaaaat tcaatgccgg
      961 tactcataca gtaattttcg atgcttcgaa cttttcaagc gtaatttatt tatataaaat
     1021 agaagcaggc aattttactt cagttaagaa aatgcaattg atgaaatagt aatttgattt
     1081 tcttttaaaa aggatttaaa tctttattgg tttaaatcct ttttctttat ccggcagtgc
     1141 tttatttttc aattagaaat ccttaaggaa aatcatgaaa aatcttttat ctgctttatt
     1201 atcaattctt tttatcacat caatttcggc acaaactatg aaaacagcat ttataaacgg
     1261 gaaagtatat acagtaaatg aaaaacagcc tcttgcccag tccgttattg ttgaaggaaa
     1321 taaaatatta tttgtaggat cggatgaaga tgcgaaaaaa ttatttgatg cctcaactaa
     1381 aatcatcgat ttaaaaggaa agttaatgct gccgggcttt attgataatc atgttcactt
     1441 cataaccggt ggattttatt tgcttggtat cgatcttcgt cctgctgcct ccacaaatga
     1501 gtttagaaag attttaaaag tttataggga aaaatatccc aacaaatgga tcactggcgg
     1561 ttactgggat catgagaaat gggaagtaaa agatctgcca acaaaagaaa tgattgacga
     1621 tatctcacct aatacaccgg tttttgttga acgattggat ggacatatgg gtcttgcaaa
     1681 ttcttatgca ttaaagcttg caggaataac aaaagacacc gaaagcccgg atggcggatt
     1741 gattgttaaa gattctaaaa ccggtgaacc cacaggtatt ctaaaagata atgcaatgaa
     1801 ttttatctat aatatactgc ctgcggcaac ggcagaagaa aattatgatg ccgcagttgc
     1861 tgcattggaa gaagcgaaac gattgggaat tacaagcgtg caggatatta catttgaaga
     1921 tgcactgaac acatttacaa aacttaaaga tgaaggtaaa cttacttgca gaatttttac
     1981 ccgctggcca attgctgatt atcaaagtct ggttaaaaaa aacattcaag ttggttatgg
     2041 tgatgaatta ataagaatgg gttcgctaaa ggcttttgca gatggttcat taggatcaag
     2101 tacagcatgg ttttttgaaa aatataatca ggatacaacc acttttggtt tgccgatgga
     2161 tattattaca aacggagaaa tggaaaagtg gtgtttagat gctgataaaa atcacttgca
     2221 gttttctgta catgctattg gagataaagc taattcctgg atgctggatc tatttgaaaa
     2281 aattactaaa gaaaatccaa aatgggatag aagatttaga attgaacacg cacagcatgt
     2341 aaggtttgaa gatataaaac gatttgccca acttggcgtt atagcttctg tgcagccgta
     2401 tcactgcatt gatgatggtg tttgggctga aaaaagaata ggacccgaaa gaattaaata
     2461 cacatatcca ttcaaatctt ttttggatgc tggagtaaaa atgtgtttcg gtactgattg
     2521 gtatgttgct ccattaaatc cattactagg gatttatgct gcggttacac gcagaacatt
     2581 ggatga
//
LOCUS       DASWBY010000514         4801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_247645, whole genome shotgun sequence.
ACCESSION   DASWBY010000514 DASWBY010000000
VERSION     DASWBY010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247645"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VF870_09245"
     CDS             <1..556
                     /locus_tag="VF870_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252416.1"
                     /translation="FTSKDNWKNGTKLSNIYTTLQEGLPPSAMIAYDFLLPEQKFALA
                     HYIRTNFIPSPEADTDADLQALDATFNLSAGLQLPAQIPVKTAGFLVISENQKANNKI
                     DSTLSSLNKYQAENGFNVFSSVVKDKKVALSFLTKNSGWKQNENVFVNLVVNNVNQNG
                     FNGKVFNLNNDEWSSLYNLMIKLI"
     gene            571..1368
                     /locus_tag="VF870_09250"
     CDS             571..1368
                     /locus_tag="VF870_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9252417.1"
                     /translation="MKNIFFINLLFVLFSLNILANEKQVEVGIDEQLGKIIPLQEYFV
                     DENGNKVLLKDLFTKPTVFAFVYYNCPGICSPLLMELTNVINKSDLVIGVDFNVVVLS
                     MDEFETPTDALTKKQTFFQAFDKNVPPESWKFLTGDSATIKKVADAAGFHYKRQGKDF
                     LHNGAFIFIDKQGKICRYLFPSYTDRKGFGILPFDFKMAVLETSEGKVSPTMAKVLQL
                     CFRYDPEGKTYVLSFTRIFGAGILILIGIFVFVFLRVKPKKDFIKER"
     gene            1377..3038
                     /gene="ctaD"
                     /locus_tag="VF870_09255"
     CDS             1377..3038
                     /gene="ctaD"
                     /locus_tag="VF870_09255"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX9252418.1"
                     /translation="MSEAIAVPKNGKEPSYLDYQGKHKGLLGWILSTDHKRIGLLYLV
                     AIISFFLVGMTFGFLMRLELIAPGRTIMDAQTYNSFFTLHGVIMIFLFVIPGIPAIFG
                     NFFLPILIGAKDVAFPRLNLLSWYLYIIGGLLAVISIILPGGPIDTGWTFYIPYSVRT
                     GTNVSLALFAAFVLGFSSILTGLNFVTTVHRLRAPGMSWFKMPLSVWSFYSTAWVQVL
                     ATPIIGITLLLVIIERAFGVGLFDPALGGDPVLFQHLFWIYSHPAVYIMILPAMGVIS
                     EIIPVFARRTIFGYKFIAMSSVAIAVFGSLVWAHHMFTAGMSNTGQFIFSLLTMVVAI
                     PSAIKVFNWTATLYKGSIELATPLLFALAFIFQFTIGGLTGVMQGVLSVDIMVHDTSF
                     IVAHFHYVMFGGTGFGLFAALHYWFPKMFGKMYNQKISKVAFWHLFVGFNTLYFPMFI
                     MGWLGMPRRYYDYLPEFQIYHVISTIGSWILVAGVLIMFTNLIKAVIGGKKITEKNPW
                     GGETLEWQVDTPPIHENFVDIPTVHEAPYVYKQNETETLKTQEVK"
     gene            3035..3655
                     /locus_tag="VF870_09260"
     CDS             3035..3655
                     /locus_tag="VF870_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEX9252419.1"
                     /translation="MSSQEQAVAHVHRDDIGARMGMWLFLFTELILFGGMFILYSVYR
                     YKYPEAFHLAAKELNTIVGTFNTAILLTSSLTMALSITAIQRKQKALSIFFQLITIVL
                     ALGFMINKYFEWTAKFHHGIYPGSETLLAKPSGEIVFFGLYYVMTGLHGLHVIIGIVL
                     IAVMMRFTSKEIITSDSYVKLESAGLYWHLVDIIWIFLFPLFYLIT"
     gene            3675..3989
                     /locus_tag="VF870_09265"
     CDS             3675..3989
                     /locus_tag="VF870_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX9252420.1"
                     /translation="MENNHEEHLELSEHNHGYGVYILVWFALMVLTGVTVAVAGINFG
                     GITIATALVIASIKTYLVLTIFMHLRTESKTFRFFVGVALLFLIISFILLFSDYSFIV
                     RN"
     gene            3992..4645
                     /gene="coxB"
                     /locus_tag="VF870_09270"
     CDS             3992..4645
                     /gene="coxB"
                     /locus_tag="VF870_09270"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9252421.1"
                     /translation="MFNGVSNFAKEVDFAFLFTLMVSVFFLVLITGLMIYFVIKYSRK
                     RNPKATNVHSNVALEVTWTLIPTILVLIMFWFGWTGYVDMSNAPKDALNIDVTAQMWK
                     WTFKYENGKTTDSLYVPVNKPVKLNLHSLDVNHAFYVPKFRIKKDVYPNQQRMIWFKA
                     EELGSFDIACAEYCGLNHSYMYNKVKVMPQKDFNAWLNSSANDSSAKNNVKSNVTGN"
BASE COUNT         1458 a          750 c          898 g         1695 t
ORIGIN      
        1 ttttacttca aaagataact ggaagaatgg gactaaactt tccaatatct atacaacatt
       61 gcaggaaggc ttaccgccaa gtgctatgat agcttatgac tttttattac cggagcaaaa
      121 gtttgcactt gctcattaca taaggactaa ttttatacca agtcccgagg cagatactga
      181 tgctgatttg caggcattgg atgcaacttt taatttatcc gcaggtttgc aattaccggc
      241 acaaattccg gttaaaacag caggcttttt agtgataagt gaaaatcaaa aagcaaataa
      301 taaaattgat tcaaccttat catcattaaa caaataccag gcagaaaatg gttttaatgt
      361 tttttcaagt gtagttaagg ataaaaaagt tgctttgtcg tttcttacta aaaactccgg
      421 ctggaagcag aatgaaaatg tttttgttaa tcttgttgta aataatgtaa atcaaaatgg
      481 atttaatggt aaggttttta atttgaataa tgatgaatgg tcatcactct ataatttgat
      541 gatcaaattg atctaggata tcacgtaaaa atgaaaaata ttttttttat aaatcttctc
      601 ttcgttttat ttagtttgaa tattttagct aatgaaaaac aggttgaggt tggaatagat
      661 gaacaactcg ggaaaatcat ccctttacag gaatattttg ttgatgaaaa tggaaataaa
      721 gttttattaa aagatctttt tacaaaaccg accgtctttg catttgttta ttataattgt
      781 ccgggaattt gcagtccgct gttgatggaa ctgacaaacg taattaacaa atcagattta
      841 gttataggtg ttgattttaa tgttgtagtt ttaagtatgg atgagtttga aactccgact
      901 gatgctttaa caaaaaagca aacatttttt caggcattcg ataaaaatgt tccacctgag
      961 agctggaagt ttttaacagg cgatagtgct acaataaaaa aagttgctga cgctgctggt
     1021 ttccattata agagacaagg gaaagatttt cttcataatg gggcttttat ttttatagat
     1081 aaacaaggga aaatttgcag ataccttttc ccaagttata cagatagaaa aggatttgga
     1141 attttgccat ttgattttaa gatggctgta ttagaaacct ccgaaggtaa agtttctcca
     1201 acgatggcaa aagtattaca attgtgtttt agatatgatc ccgaaggcaa aacctatgtg
     1261 cttagcttta ccagaatatt tggtgcaggc atcttaatat tgataggaat atttgttttc
     1321 gtcttcttaa gagttaaacc caaaaaagat tttattaaag agaggtaagt ttataaatga
     1381 gtgaagcaat cgcggttcct aaaaatggaa aagaacccag ctatcttgat tatcaaggta
     1441 aacacaaagg tttactgggc tggatacttt ctactgatca caaaagaata ggattacttt
     1501 atttagttgc aatcatttct ttctttttag ttggaatgac atttggattt ctgatgcggc
     1561 ttgaattgat cgcaccaggc agaactataa tggatgccca aacatataat tcgttcttta
     1621 cactgcacgg tgtaataatg attttcttgt ttgttattcc cggaattcct gccatattcg
     1681 gcaacttttt tcttccgatt ttaattgggg caaaagatgt tgcattccca agattaaatt
     1741 tactttcatg gtatctttat atcataggtg gattacttgc agtaatttca attatactgc
     1801 ctggaggtcc aattgataca ggctggacat tttacatccc atacagcgta agaacaggta
     1861 caaatgttag cttagctctt tttgcagctt ttgtacttgg gttttcatca attcttaccg
     1921 gattgaattt tgtaaccaca gtacatcgtt taagagcgcc cggaatgagt tggtttaaga
     1981 tgcctctttc cgtttggtca ttttactcta ctgcctgggt tcaagtttta gctacaccaa
     2041 taattggaat cacattactt cttgtaataa tagaaagagc atttggagtt ggattatttg
     2101 atcctgcact tggcggtgat ccggttttat tccagcatct attttggatt tattcacatc
     2161 ccgctgttta tataatgatt cttcctgcga tgggtgttat ttcagaaatc attcctgtct
     2221 ttgcaagaag aacaattttt ggatataagt ttatagcaat gtcaagtgtt gctatagccg
     2281 tatttggctc attggtttgg gctcatcata tgtttactgc aggaatgagt aataccggac
     2341 aatttatttt ttcactgctc actatggtgg ttgctattcc aagtgcaata aaagttttta
     2401 actggacggc aactttatat aaagggtcta ttgaattagc tactccttta ctttttgctc
     2461 tcgcatttat ctttcaattt actattggtg gattaaccgg agttatgcag ggtgttcttt
     2521 cagttgatat tatggttcac gatacttctt tcatagtagc acattttcat tatgtaatgt
     2581 ttggcggaac aggttttgga ttatttgctg cactacatta ctggtttccc aaaatgtttg
     2641 gaaaaatgta caatcaaaaa ataagcaaag ttgcattttg gcatttattt gtaggcttta
     2701 atacattgta ttttccgatg ttcataatgg gttggcttgg aatgccaaga agatattatg
     2761 attatcttcc tgaattccaa atctatcatg tgatatctac aattggttcc tggattctgg
     2821 ttgcgggtgt gttaataatg tttaccaatc tgataaaagc tgttataggt gggaagaaaa
     2881 ttaccgagaa aaatccttgg ggcggcgaaa cacttgaatg gcaggttgat actccaccga
     2941 ttcatgaaaa ctttgttgat attccaactg tgcatgaagc cccttatgta tataaacaaa
     3001 acgaaactga aactctaaaa actcaggagg ttaagtgagt tcacaagaac aagctgttgc
     3061 tcacgtgcat cgtgatgata ttggcgcacg aatgggaatg tggctgttcc tctttacaga
     3121 attaattttg ttcggaggta tgtttattct ttattctgtt taccgttata aatatcctga
     3181 ggcttttcat ttggcggcta aggaactaaa cacaattgtt ggaactttta atacagcaat
     3241 tctgcttaca agttcattaa caatggcgct ttcaataaca gctatacaaa gaaagcagaa
     3301 agcactttcg attttctttc agttaattac gatcgtttta gcacttggat ttatgatcaa
     3361 taagtatttt gaatggactg caaaatttca tcatggtatt tatcctggtt cggaaacatt
     3421 gcttgctaaa ccttccggcg aaattgtttt cttcggtttg tactatgtta tgaccgggtt
     3481 gcacgggttg cacgttatta taggtatagt tttaattgca gtaatgatgc gatttacttc
     3541 caaagaaatt attacaagtg atagttatgt aaaattggaa tccgcaggat tgtattggca
     3601 cctggttgat ataatctgga ttttcttatt cccactattc tatctgatta cataagattt
     3661 taaggtataa atatatggaa aataatcacg aagaacattt agaactctct gaacacaatc
     3721 acggctatgg tgtttatatt cttgtttggt ttgctctgat ggttcttaca ggagtaacag
     3781 ttgctgttgc cggaattaat tttggcggaa taacaattgc aaccgcctta gtaattgctt
     3841 caataaaaac ttatttggtg ctcacaatat ttatgcactt aagaactgaa agcaaaacat
     3901 tcagattttt tgttggcgtt gcattactgt ttctaataat ctcttttatt ttattattct
     3961 ctgattattc ttttattgta aggaattaaa tatgtttaac ggtgtgtcca attttgcaaa
     4021 agaagtagat tttgcttttc tttttactct gatggtatcg gtcttttttc ttgtactaat
     4081 tacaggatta atgatctact ttgttataaa gtacagccgc aaaagaaacc cgaaagcaac
     4141 taacgttcat agtaatgttg cgttagaggt aacatggaca ctgattccta caatacttgt
     4201 tttaattatg ttttggtttg gctggactgg atacgtagat atgtctaatg cccctaagga
     4261 tgcgctcaac attgatgtaa ctgctcaaat gtggaaatgg acttttaaat atgagaacgg
     4321 taagaccact gattcgcttt atgttccggt aaacaaaccc gtaaaactta atctgcattc
     4381 tttggatgtt aaccatgctt tttacgttcc caaattcaga ataaaaaaag atgtttatcc
     4441 aaatcaacag aggatgatat ggtttaaagc tgaagaatta ggttcttttg atattgcttg
     4501 cgccgagtac tgcggattaa atcattccta tatgtataat aaagttaaag ttatgccgca
     4561 gaaagacttt aatgcctggt tgaattcctc agctaatgat tcttctgcaa aaaataatgt
     4621 caaatctaat gtaacgggta attaaattgc tgagagaaca aatcaacata ttatttgaga
     4681 tcactaaact ccgaattaca atttttgtta cttttacaac tgtttttgga tacattgctg
     4741 ctaccaattc gattgacttc gggttgattc ttccaacgat tggaatatta ctattagcct
     4801 g
//
LOCUS       DASWBY010000515         3744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_95022, whole genome shotgun sequence.
ACCESSION   DASWBY010000515 DASWBY010000000
VERSION     DASWBY010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95022"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VF870_09275"
     CDS             complement(<1..282)
                     /locus_tag="VF870_09275"
                     /inference="COORDINATES: protein motif:HMM:NF042518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6567 family protein"
                     /protein_id="HEX9252422.1"
                     /translation="MKKFILIPIVIFVSLIISSCSSTPEITNQNQTEVTQNTNKYGIV
                     SEMLEEARQYYVLALKKQELNSTKETVENYEAALRIVNNLSYYPGIDENA"
     gene            complement(394..1938)
                     /locus_tag="VF870_09280"
     CDS             complement(394..1938)
                     /locus_tag="VF870_09280"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9252423.1"
                     /translation="MKNKLDELSQKKETAKLGGGEQKIKNQHDKGKLTARERIELLVD
                     EGSFDEIGMFVKHRAKDFGLDKQNYPGDGVITGFAKIDGKPVALFSQDFTVFGGSLSE
                     THAEKIVKIMKLAMKAGIPIIGLNDSGGARIQEGVNSLGGYADIFLLNTLASGVVPQI
                     SVILGPCAGGAVYSPAITDFVFMTRKTSHMFVTGPNVVKTVTHEDVTFEDLGGADTHS
                     QKSGVAHFAFDNEIETLLNVKKLLSYIPLNFKEKAENTDFDFDSLKENEELCSIIPDS
                     PNKPYDMKEVILNLVDDEVFFEVHKYYAENIICGFARLGGMSVGIVANQPQVLAGVLD
                     INASVKSARFVRFCDAFNIPLLVLVDVPGFLPGTEQEWNGIIRHGAKLLFAFSEATVP
                     KVTLITRKAYGGAYDVMNSKHIRGDFNYAWPSAEIAVMGPKGAVEIIFKKEIESSKNP
                     EQELESKLNEYIQKFANPYIAAERGFLDDVIDPKITKKVLINAFELLKDKVDLNPRKK
                     HNNMPL"
     gene            complement(1954..2598)
                     /locus_tag="VF870_09285"
     CDS             complement(1954..2598)
                     /locus_tag="VF870_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9252424.1"
                     /translation="MIQIKKFTFNLFSENTFVLWDDDSRECAIVDPGCSDPSEENELE
                     SFITDDNFKVKYLINTHCHIDHILGCDFVKNKFNPIYYAPELDLPLLQNANMQASFAG
                     MDFSISLRPDELLSESKKLFLGKTELNFLFTPGHTPGEFCIYIPQIKSCITGDVLFYD
                     SIGRTDLWGGDYDILIKTINEKLLSLPDETIIYPGHGENSTIGREKKSNPFLNK"
     gene            complement(2601..>3744)
                     /gene="aroC"
                     /locus_tag="VF870_09290"
     CDS             complement(2601..>3744)
                     /gene="aroC"
                     /locus_tag="VF870_09290"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX9252425.1"
                     /translation="SNLKISENYINQQLKRRQFGYGRGLRMKIESDKAEILSGVRFGK
                     TLGSPISMLIRNADWNNWQTLMSVDPINQKVEKISIPRPGHADLVGVSKYNFDDIRSS
                     IERSSARETAARVAAGSIARKLLEEFGITIGSFVESIGGIYPEKNFTEKLFNNSIASN
                     FSAQSISDKADKSLVRVLESGQEDKIIRKIKTAKKNGDTLGGTFITFATGIPVGLGSF
                     MNYDTKLDAEIAHSIMSINAVKAVEVGEGFLFAEKFGSKSHDEIVIKKDFIGRKTNRA
                     GGIEGGVSTGLPIIVRAAMKPIATLMSPIMSVDLSKMKKIDARRERSDFVAVPACAVI
                     AESMLAWSIAKFFLQKFGGDSIEELKDNYTTYNKKLFSRIKNNFKS"
BASE COUNT         1139 a          737 c          551 g         1317 t
ORIGIN      
        1 tgcgttttca tcaattccgg gataataact taaattgttt acaattctta aagccgcttc
       61 ataattctca acagtttctt tcgtactatt taattcctgt ttttttaaag ccaagacata
      121 atattgtcta gcttcttcaa gcatttcaga aacaatccca tatttatttg tgttttgtgt
      181 tacttcggtt tgattttgat ttgttatctc tggtgtggat gagcaggatg agatgatcaa
      241 tgaaacaaaa ataactattg ggatcagaat aaatttcttc attagtactc cggaataaat
      301 tttggcttat gcaatataaa tgttcatttt ggtttaatca agtaatattt tagatcaacc
      361 gagttaaatc ttagttttat aatcacttaa aaattataaa ggcatattat tatgtttttt
      421 tctgggattc aaatctactt tatcttttaa caattcaaat gcattaatca atactttttt
      481 tgtaatcttt ggatcgatta catcatccaa aaatcctcgt tcagcagcta tgtaaggatt
      541 agcaaatttt tgtatgtatt cattaagttt gctttcaagt tcctgctctg gatttttaga
      601 tgattcaatc tccttcttaa aaataatttc tacagcacct tttggtccca taactgctat
      661 ctctgctgat ggccaggcat aattaaaatc gcctcgaata tgctttgagt tcataacatc
      721 gtaagcacca ccatatgctt ttcttgtaat caaagtaact tttggaacag tagcttcact
      781 aaatgcgaac agcagttttg ccccatgtct aattatccca ttccattcct gttctgtgcc
      841 aggcaaaaat cccggaacat ctaccaaaac aagcaaagga atgttaaaag catcacaaaa
      901 acgaacaaat cttgcacttt ttaccgaagc attaatgtcc aaaactcccg ccaaaacttg
      961 cggttgattt gcgacaattc cgacactcat ccccccaagt cttgcaaaac cacaaattat
     1021 attttcagca taatatttat gaacttcaaa aaaaacttca tcatcaacca aatttagaat
     1081 tacttctttc atatcataag gcttgttcgg gctgtctgga ataattgagc aaagttcttc
     1141 attttctttt aatgaatcaa aatcaaaatc ggtattctca gctttttcct taaaattaag
     1201 agggatataa ctcaacaatt tttttacgtt taataaagtt tctatttcat tatcaaaagc
     1261 aaaatgtgca acgccgcttt tttgagaatg agtatcggca cctccaagat cttcaaaagt
     1321 aacatcttcg tgagtaacag tttttacaac gttcggtcct gttacaaaca tatgacttgt
     1381 cttcctggtc ataaaaacaa aatcagttat tgccggtgag tacacagcac cgcccgcaca
     1441 cgggccaagt attacagata tctgtggtac tactccggat gcgagtgtat ttaataaaaa
     1501 tatatctgca tatcctccca gactatttac tccttcctga attcttgcac caccggaatc
     1561 atttaatcca ataattggta tgcctgcctt cattgcaagt ttcataatct taacaatttt
     1621 ttctgcgtgt gtttcagaaa gtgagccgcc aaacactgta aaatcctgtg agaataaagc
     1681 aaccggtttc ccatctattt tagcaaagcc ggttattacg ccatcgccgg gataattttg
     1741 cttgtctaat ccaaaatctt tagcacggtg tttaacaaac ataccgatct catcaaaact
     1801 tccttcatca actaataatt ctattctttc acgagcagtt aactttcctt tatcatgctg
     1861 gtttttaatt ttttgttcgc cgccgccaag ttttgcagtt tctttttttt gcgatagttc
     1921 atccaattta tttttcatga aattcctgaa ggattatttg tttaaaaatg gatttgattt
     1981 tttttcgcga ccaattgtac tgttttcacc atgtccggga taaataattg tttcatccgg
     2041 tagagataaa agtttttcat taatagtttt aatcagaatg tcataatcac ctccccataa
     2101 atcagttctg ccgatgctat catagaaaag gacatcaccg gtaatgcagg atttaatttg
     2161 tgggatataa atacaaaact cccccggtgt atgtcccgga gtaaaaagaa aattcaattc
     2221 tgtttttcct aaaaaaagtt ttttactttc tgataataat tcatcaggtc ttaatgaaat
     2281 agaaaaatcc atcccggcaa aacttgcctg catatttgca ttttgcagaa gtggtagatc
     2341 aagttccggc gcgtaataaa tcggattaaa tttattttta acaaaatcgc agcctaaaat
     2401 atgatctata tgacagtgcg tgttgatgag atatttaact ttaaaattgt catctgtaat
     2461 aaagctttca agttcatttt cttcggatgg atcactgcag cctggatcaa caatcgcaca
     2521 ttctctagag tcgtcatccc ataatacaaa tgtattttcc gaaaataagt tgaatgtaaa
     2581 tttctttatt tgaatcaata ttaactctta aaattgtttt taattctgct gaataatttt
     2641 ttattataag ttgtgtaatt gtcttttaac tcttctatgg agtcaccgcc aaatttctgt
     2701 aaaaagaatt ttgcgatact ccatgccaac atagattcag caattactgc acaagcaggt
     2761 acagcaacaa aatcacttcg ctctcttctt gcatcaattt ttttcatttt tgataaatct
     2821 acgctcatta ttggtgacat taaagtggca attggtttca tagcagcacg aacaattatt
     2881 ggcaaacctg ttgaaactcc gccttcaatt ccaccagcac ggttagtttt tctgccgatg
     2941 aaatctttct ttattacaat ttcatcgtga gattttgatc caaacttttc tgcgaataaa
     3001 aatccctctc caacctcaac tgcttttacc gcattgattg acataattga atgcgcaatt
     3061 tcagcatcca gttttgtatc ataattcata aaactgccaa gaccgaccgg aatacctgtt
     3121 gcgaatgtta taaatgttcc gccaagtgta tctccatttt tctttgctgt ttttattttc
     3181 cgaatgattt tatcttcctg ccccgattcc agaacacgaa ctaaactttt atcagcctta
     3241 tcagaaattg attgagcaga gaaatttgat gcgatcgagt tattaaaaag tttttcagta
     3301 aaattctttt caggataaat tccccctatg ctttcaacaa aacttccgat tgtaattcca
     3361 aattcttcaa gcaattttct tgctattgat cctgcggcaa cacgggcagc tgtttctctt
     3421 gcggatgatc gttcgataga gcttcttata tcatcaaaat tgtattttga aactccaact
     3481 aaatcagcgt gaccaggtcg agggatagaa attttttcaa ctttttgatt tattggatca
     3541 actgacatta atgtttgcca attattccag tcagcattcc tgatcaacat agatattgga
     3601 gaacctaatg ttttaccaaa tctaactccg gataaaattt cagctttatc agattcaatt
     3661 ttcatgcgca aaccacgacc gtagccgaac tgcctgcgct tcaactgctg attaatataa
     3721 ttttcagaaa tttttaagtt tgat
//
LOCUS       DASWBY010000516         1949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_23803, whole genome shotgun sequence.
ACCESSION   DASWBY010000516 DASWBY010000000
VERSION     DASWBY010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23803"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VF870_09295"
     CDS             complement(<1..823)
                     /locus_tag="VF870_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252426.1"
                     /translation="MNNKRICLVTSGHPPIDERIFWKFSKSLKDAGFSVSIICSTQDI
                     NKVVDAISLRGFDGTSFSKKKKITEFYNLINLFNPDLLLCSEMLPVFAALKFTKENPD
                     TKIILDITEWFPENVAFKFHGIKRWIKYFQLLLPYIYVLQKVDHLIIGEISKKKRYDF
                     LAGSKPKTIIGYYPVLKYFNYKKPDLSKEEIILGYAGVITFERGINKILQLSLSLANK
                     FPLKKFRLLLFGKFTYPNEETEFKQKISTINNLDVEFVDWTDYDKMSSVIERMDIC"
     gene            881..1636
                     /locus_tag="VF870_09300"
     CDS             881..1636
                     /locus_tag="VF870_09300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9252427.1"
                     /translation="MHTQNKISVVIITGNEENNIKDCLKSVKWANEIIVVDSESTDNT
                     VKIAKEFTDKVIAHKWEGYASQKAFAIALSKNEWVLSLDADERVTESLAKEILETELT
                     PHNGYFIRRDNFFLGKLIRGCGWGNDLQLRLFKKSETKLSDRMVHEKFIVNGSTSYLK
                     NSFSHYSYTNLKDAFVKINNYSTLEANEKAGKKTVNLFTLLFSPPFYFLHHLIIRKGF
                     LDGIYGVIISYMHMVTKLQVQLKIMEIKKNKKS"
     gene            1641..>1949
                     /locus_tag="VF870_09305"
     CDS             1641..>1949
                     /locus_tag="VF870_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HEX9252428.1"
                     /translation="MKQLRFLIARIDRIGDVVLSTPLPREIKKKYPNSFVAVLVRHYT
                     KDIYLNNPNVDQIITIDNTDKTEKSFWNLVTEIKQYKFTHAFMLLPNEKINYTLFMSG
                     I"
BASE COUNT          690 a          299 c          315 g          645 t
ORIGIN      
        1 aacaaatatc cattctttca ataactgaac tcattttatc gtaatctgtc caatccacaa
       61 attccacatc caaattatta atagttgata ttttctgctt gaattctgtt tcctcatttg
      121 gataggtgaa tttcccaaac aataaaagcc tgaatttctt tagtggaaac ttattagcta
      181 atgaaagcga caactgcaaa attttattaa ttcctcgctc gaatgtgatt accccagcat
      241 atcccaaaat tatttcttct ttagatagat caggtttttt ataattgaaa tatttgagta
      301 ccggataata cccgattata gtttttggtt tcgaacctgc aagaaaatca taacgttttt
      361 tcttagatat ttccccgata attaagtgat caactttttg caacacataa atatagggga
      421 gtaataattg aaaatatttt atccatcgtt tgatgccgtg gaacttaaat gcaacatttt
      481 ccggaaacca ttctgtaatg tctagaataa tttttgtgtc tggattttct tttgtgaact
      541 ttaatgctgc aaaaacggga agcatttcgg aacagagtaa taaatcggga ttgaaaagat
      601 taatcaaatt ataaaactca gtaatctttt tcttctttga aaacgaagtt ccgtcaaatc
      661 cgcgaagact aattgcatca acaactttat taatatcctg agtagagcag atgattgaga
      721 ctgaaaatcc ggcatccttt aatgatttag aaaatttcca gaaaatcctc tcatcaattg
      781 gtggatggcc ggaagtaact aaacagattc gtttattatt caagaacttt tactctttat
      841 tttattaaat ttgaaccaac aattgaataa atcaaaattt atgcacacgc aaaataaaat
      901 atctgtggtc attattacag gcaatgaaga aaataatatt aaagattgct taaaaagcgt
      961 taaatgggcg aatgagataa ttgttgtaga ttctgaaagc actgacaata cggtcaaaat
     1021 tgcaaaagag tttactgata aagtaattgc tcataaatgg gaaggatatg cgtctcaaaa
     1081 agcttttgca attgcattat ctaaaaatga atgggtacta agcctggatg cagatgaacg
     1141 tgttaccgaa tctttagcaa aagaaattct tgaaactgaa ttgactccgc acaatggata
     1201 ctttattagg cgtgataatt tctttttagg gaagctaatt cgcggatgcg gctggggaaa
     1261 tgatttgcag ctgcgattat ttaaaaaatc agaaacaaaa ttgtccgata gaatggtgca
     1321 tgaaaaattt attgttaatg gaagtacatc atacctcaaa aatagttttt cacattattc
     1381 atacactaat cttaaagatg cgtttgttaa gataaataat tactcaactc ttgaagcaaa
     1441 tgaaaaagct ggtaagaaga ctgttaacct atttaccctt ctgtttagtc cgccatttta
     1501 ttttctgcat catttaataa tccgtaaagg ttttttagac ggaatttatg gagttatcat
     1561 ttcttatatg catatggtta caaaacttca ggtacagctt aagattatgg aaataaagaa
     1621 aaataaaaaa tcgtaatctt atgaagcagt tgagatttct tatcgcaaga attgacagaa
     1681 taggcgatgt tgtgctttct actccgctgc cgcgtgaaat caaaaaaaaa tatccgaata
     1741 gttttgttgc tgttttagtc aggcattaca ctaaagacat ttatctaaat aatcctaacg
     1801 tagatcaaat aattacgatt gataatactg acaagactga aaaaagtttt tggaatttag
     1861 taacagaaat aaagcagtat aaatttacgc atgcattcat gctcctgcca aatgaaaaaa
     1921 ttaattatac tctttttatg tctggaatt
//
LOCUS       DASWBY010000517         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_55793, whole genome shotgun sequence.
ACCESSION   DASWBY010000517 DASWBY010000000
VERSION     DASWBY010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55793"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..1306
                     /locus_tag="VF870_09310"
     CDS             332..1306
                     /locus_tag="VF870_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9252429.1"
                     /translation="MKHVSIIVTKGAILGNIEGPRQVFTEVNHLLQSRGQSAFFEIEL
                     VGLSKASLLNDGLYTISTRSIYDIEKTDLIIIPAMYGELEKAIVANKDFIPWIIKHYK
                     NGSEVASLCLGAFLLASTGILNGRKCATHWLAANEFKKMFPKVNFQEDKIITDENGIY
                     SSGGAYSSLNLILYLVEKYAGREAAIFCSKAFQIDIQRDSQSPFTIFIGQKDHEDEPV
                     KKAQEYIEINYNQKITVDQLAIMFALSRRNLERRFKKATSNTIIEYIQRVKIEAAKKN
                     LESARKSISEVMYEAGYSDNKAFRTIFRKYTGISPIQYRNKYNRELVA"
     gene            1318..1440
                     /locus_tag="VF870_09315"
     CDS             1318..1440
                     /locus_tag="VF870_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252430.1"
                     /translation="MNINRVLDFQSNAGTVNRKMLAMIIKLELGYNFKVQRLKK"
BASE COUNT          609 a          255 c          287 g          494 t
ORIGIN      
        1 tttatctgaa tcgggtgtgt ctttaaagcg gaacattcca cccaaaaagt ctccaccgaa
       61 aattttttta taaaagataa atgcctcttc cgtgttacct ggaaaattca gatacggatt
      121 gattgatttc atgactgcct cctttatttg ttaacgacaa aataattaat gttaaacaat
      181 tattgattgt agtaatgcaa atatatacca agaaaccctt tgcttgtcag gtgtaattac
      241 ggcattctca agggttgaat gcgacatagc aattatataa tttctagtta taaataatat
      301 ccaacaatta ttaataattg gcaatacaat aatgaaacac gtttcaataa tagtgacaaa
      361 aggggcaatt cttggaaata ttgaaggccc acgtcaggta tttactgaag tgaaccacct
      421 tcttcaatcc cgcggtcaat cagcattttt tgaaattgag cttgtgggac tttcaaaagc
      481 atcacttctc aatgatggat tgtacacaat ttctacaaga tcaatttatg acattgaaaa
      541 aacagatctg ataattatac ctgcaatgta tggagagctt gaaaaagcaa tagtagcaaa
      601 taaagatttt attccatgga taataaagca ttataaaaac ggatcagagg ttgcaagttt
      661 gtgccttggc gcttttttat tagcatcaac tggaatactg aacggtagaa aatgtgcaac
      721 tcactggttg gctgcaaatg agttcaaaaa aatgtttcct aaagttaatt ttcaggaaga
      781 taaaattatt acagacgaaa atggtattta ctcaagcggt ggtgcatatt cttctttaaa
      841 cctgattttg tacctggttg aaaaatacgc aggaagagaa gctgcaattt tttgttcaaa
      901 ggcatttcaa attgatattc agagagatag tcagtcgcct tttacaattt ttatcggaca
      961 gaaagatcat gaagatgaac cggtaaaaaa agcgcaggag tacattgaga taaactataa
     1021 tcaaaaaatc acagtagatc agcttgcaat aatgtttgca ttaagcagaa gaaaccttga
     1081 aagacgattt aagaaagcaa cctcaaatac aataatagaa tatatccaga gagttaagat
     1141 tgaagctgcc aagaaaaatc ttgaatcagc aagaaaaagt attagtgagg taatgtatga
     1201 agcaggttat tctgataata aagccttccg aacaattttt agaaaatata ccggaatatc
     1261 tccgattcag tacaggaata aatataacag agaattagtc gcatagtatt tttaaatatg
     1321 aatattaaca gagtgctgga ttttcaaagc aatgccggta cagtaaacag aaagatgctg
     1381 gcaatgataa ttaaattaga gcttggctat aatttcaaag ttcagcgact gaaaaaataa
     1441 ttcgaaaaac aaaaatccct taaacctact cttcaaaaat ttgtcacgtc ataagaagga
     1501 ttgccttaga ttcagcgatt ggttaactct ctttcgtccg caccttctga cttttcagca
     1561 gatggctcga agcaggttat taagttcatt ttaaacataa gtaaataata tataattgat
     1621 tcataaaaga ctttttacta taata
//
LOCUS       DASWBY010000518         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_475058, whole genome shotgun sequence.
ACCESSION   DASWBY010000518 DASWBY010000000
VERSION     DASWBY010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475058"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1077)
                     /locus_tag="VF870_09320"
     CDS             complement(205..1077)
                     /locus_tag="VF870_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9252431.1"
                     /translation="MTYWYLYLIIPVVLILFAGIRIVRPTHRGLIERLGKYHNFASPG
                     FHLIIPIIDRLFPVNVTEQMVDAQPQEIITNDNLNASVDAQVYFRVKADEESVKSSIY
                     NVNNYKWQIVNLARTTLRNIIGTLTLKSANSERGKINAELYRTLHTETQTWGIEIVRT
                     ELKEIDPPSDVQDTMNKVVKAENEKIAAIDSATAAETVADGVKRAKIKEAEGYKQSKI
                     LHAEGEAEAIRLVNEAADKYFVGNAQLLRKLEALEASLGSNAKIVIPAGSELVNIIGE
                     MAGIIPLERKKKDG"
     gene            complement(1150..1320)
                     /locus_tag="VF870_09325"
     CDS             complement(1150..1320)
                     /locus_tag="VF870_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252432.1"
                     /translation="MLTIFTTVTFFTRERVAKIGDINITANKRHNLKWSPLVGIAVMA
                     IGGVIVWQSPKK"
     gene            complement(1508..2071)
                     /locus_tag="VF870_09330"
     CDS             complement(1508..2071)
                     /locus_tag="VF870_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252433.1"
                     /translation="MNNEEQNFTDPQMLRKKAEEQLRKKQKKVSKPVIETDVKKLLHE
                     LQVHQIELEMQNDELHQAYEIAEAALKKYTMLYDFAPMGYFTLESDGSICDLNFTGAE
                     MLGDKRFSLVNSNFKLFISEESQAVFNDFLSKVYTNNTKESCEVKLGYNNNPLCTAYM
                     EGIVTGDDRKCLLSVVDISSFKKQDNS"
     gene            complement(2079..>2567)
                     /locus_tag="VF870_09335"
     CDS             complement(2079..>2567)
                     /locus_tag="VF870_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9252434.1"
                     /translation="QKQIATRDGRWFSVRIMPYRTFDDRIDGLVITFINISDLKQVEV
                     KYHETAQLNRLLLNSSSDVIIKLSTDLKILEYNTEADKFFGKKHEDTVNQYFIHTFIP
                     EPARKKTEKGMNEMLNRLTGGKFKMQVIAAGGNVRVVEWSVNVLVNSLKTATGMIIII
                     KK"
BASE COUNT          690 a          534 c          449 g          894 t
ORIGIN      
        1 aaggaaaccc gaagaattta caagcgctac ttcgatcttt gtacctccaa gatcaatgcc
       61 gattgcccat tgatctgtat tcatcagatt ctgctaattg taattctgat ttattacctt
      121 ccggatccgg tatttttgta agacgccgga tgaaacattc atcgccatgg aaacgagcgc
      181 tgagaaataa taatggagta atatttaacc atcttttttc tttctctcca atggaattat
      241 tcctgccatc tctccaatga tatttaccaa ctcgcttcct gccggaataa caattttagc
      301 attactcccc agtgatgctt caagagcttc cagttttctc aataattgcg catttccaac
      361 aaaatattta tctgccgctt catttaccag cctgattgct tcggcttctc cttcggcatg
      421 cagtattttc gattgtttat atccttctgc ttctttaatc tttgcccgtt tgacaccgtc
      481 ggcaacggtt tctgctgctg ttgcagaatc tatagctgct attttttcat tttcggcttt
      541 taccacttta ttcatggtat cctgtacatc acttggcggg tcaatttctt tcaactctgt
      601 ccgtactatt tctatacccc aggtttgggt ttcggtatga agtgttcggt agagttctgc
      661 attaatctta ccccgttcgc tgttggccga ctttagtgta agtgtaccaa taatattacg
      721 caatgttgtg cgggcaagat taactatctg ccatttgtaa ttattaacat tgtagattga
      781 acttttaacg ctttcttcat cagctttcac cctgaaatat acctgtgcat caacgcttgc
      841 attcagatta tcatttgtga ttatttcctg tggctgcgca tctaccatct gctcggtcac
      901 atttaccggg aatagtctgt caatgatcgg aataatcagg tgaaatccgg gactggcaaa
      961 attatgatac tttcccagcc tctcaattaa acctctgtga gttggccgta caattcttat
     1021 tcctgcgaat aatatcaata caacaggaat tatcagatac aaataccaat aagtcatttc
     1081 aatttccttt ctttttttgt aattatactt cgtaaatact tatataatca ggagtttaac
     1141 ctgtagcagc tattttttag gggactgcca tacaataact ccgccgattg ccattactgc
     1201 aatgccaacc agtggagacc atttgaggtt atgccgtttg tttgcagtta tattaatatc
     1261 cccgattttc gctactctct ctctggtgaa gaaggtaaca gttgtaaaga tggttaacac
     1321 taaaccaatc agaatgaggg catacccaac ttttttcata tcgaatttta ttatgaataa
     1381 ttttaacgaa aggtaggtta aggtgaaaga aaaacgttac acaattattt aaaagggtta
     1441 catcattcac acattaacct gaacggtgcg ctaattgtaa aaatttggag aggtgtggaa
     1501 gaaatgttta agaattgtcc tgttttttga aacttgaaat atcaaccacc gacaaaaggc
     1561 atttccggtc atcgccggta actatacctt ccatataagc ggtgcataaa ggattattgt
     1621 tataaccaag cttcacttcg catgattctt tggtattatt ggtataaact ttactgagaa
     1681 agtcgttaaa tacagcctga gattcttcag aaatgaacag cttaaagttg ctgtttacca
     1741 gactaaagcg tttatcacca agcatttcgg caccagtgaa gttcagatcg cagatactgc
     1801 cgtcgctttc gagtgtaaag tagcccatcg gtgcaaaatc gtacaacatg gtatattttt
     1861 taagagccgc ttctgcaatt tcataagcct gatgcaactc atcgttctgc atttctaatt
     1921 ctatctgatg tacctgaagt tcatgcagga gtttcttcac atcggtctct ataaccggtt
     1981 tgctaacttt tttttgcttc ttcctgagtt gttcttcagc ttttttgcgc agcatctggg
     2041 gatctgtaaa attctgttct tcattattca tagctaagtt atttttttat tataataatc
     2101 attccggttg ctgttttcag tgagttgacc aacacattta cagaccattc aacaactctt
     2161 acattgcctc cggctgctat tacctgcatt ttgaatttgc cacccgtcaa tctgttaagc
     2221 atctcattca tacctttttc agtctttttt cgcgctggtt ccggaataaa cgtatgaata
     2281 aaatattggt ttactgtatc ctcatgtttt ttaccaaaaa atttatcagc ttcagtatta
     2341 tattccagta ttttcaagtc ggttgataat ttaataatta catctgatga ggaatttagg
     2401 agcaaacgat tcagctgcgc tgtttcgtgg tatttaactt ctacctgctt tagatcagag
     2461 atgttaataa aagtgataac caggccgtct attctgtcat cgaaggtgcg ataaggcata
     2521 atgcgaacag aaaaccatcg tccgtctctg gtggcaattt gcttttg
//
LOCUS       DASWBY010000519         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_699265, whole genome shotgun sequence.
ACCESSION   DASWBY010000519 DASWBY010000000
VERSION     DASWBY010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699265"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..380
                     /gene="rbfA"
                     /locus_tag="VF870_09340"
     CDS             9..380
                     /gene="rbfA"
                     /locus_tag="VF870_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX9252435.1"
                     /translation="MAYRIDKVEHLIKQEISLIFLHKLSHVDLGFITITNVRVSPDLK
                     LAKIYLSVFEKEKRELVLSKIEDKKGFIRSELAHRIRIKFIPELKFFIDDTLDYVEKI
                     EGLIKKIHEDDNKTDEGSPDS"
     gene            343..1050
                     /gene="truB"
                     /locus_tag="VF870_09345"
     CDS             343..1050
                     /gene="truB"
                     /locus_tag="VF870_09345"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX9252436.1"
                     /translation="MITKQTKDPRTLDFLAGQVILIDKPVNWTSFNVVSRVRRLTKVK
                     KVGHAGTLDPLATGLLIVCTGKKTKEISEYQAQRKKYTGIITLGKSSPSMDLATEITE
                     SVLPADLNEEKILSARNKFLGNIEQVPPMFSALKHKGKTLYKLARAGKEVDRASRQVE
                     IYSFDITRIDLPDIHFEISCSKGTYIRVIADDFGKELGCGGVLSLLRRTEIGDYKVDD
                     AFDLDEFTAEFSEVQSS"
     gene            1186..2124
                     /locus_tag="VF870_09350"
     CDS             1186..2124
                     /locus_tag="VF870_09350"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX9252437.1"
                     /translation="MQVFNSIKDLTKNKNTVLTLGTFDGIHPGHLKIIDKLVDSSKEK
                     SYRNVVITFYPHPRTVLSPGGEVKMLTTQEERKYLLEKHGVEIFLTINFTKEFASLTA
                     EEFIYDYLINGIGLSEIVLGHDHHFGKGRTGNVELLKKIGAKENFSVTTVDAFYINDV
                     VVSSTKIRNALNDGDLKKANILLGRNYSFSGTVIGGDKRGRELGYPTANIKLSAQEKL
                     LPAIGIYAVRVLLENEYHFGLLSIGKRPTFYNEGDLVTEVYLYNFNREIYGELVTIEL
                     VERLRGEEKFNSAEELINQMNKDKENGLKIFNELNN"
     gene            2176..2439
                     /gene="rpsO"
                     /locus_tag="VF870_09355"
     CDS             2176..2439
                     /gene="rpsO"
                     /locus_tag="VF870_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9252438.1"
                     /translation="MTKEEKLEIISKYGKDSNDSGKSEVQIALLTKRINDLTSHFDAH
                     KKDHHSRRGLMMMVGKRRRLLDYLIKKDIARYRAIIKDLNIRK"
     gene            2450..>3420
                     /locus_tag="VF870_09360"
     CDS             2450..>3420
                     /locus_tag="VF870_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX9252439.1"
                     /translation="MVVKKEVEIGGRVFSIETGKYAKQANGSVMVRYGDTMVLVTAVA
                     ASEAKEGLDYFPLQVEYREKTSAAGKFPGGYIKREGRPTEKEILSSRLCDRPIRPLFP
                     ASFTNETQVVAMVMSYDGENDADVLAACGASAALAISDIPFDGPMAEVRVGRINGGLI
                     VNPTHEQIKLGDIELVVAGTADSIMMVEGESKEVSEQELLDALKFAQQEIKKIVELQN
                     QLVAEAGKTKWTVEEKTVDANLKKEVYDLAEAKFKEIVYSILAKEERASKNKELSESV
                     KQALAEKYPEQEKVIGEILHDMEKDLMRERILKEGVRLDGRNTTQVRP"
BASE COUNT         1245 a          466 c          706 g         1003 t
ORIGIN      
        1 gaaaataaat ggcttacaga attgataaag ttgaacatct tattaagcaa gagattagtc
       61 ttatttttct gcataaactt tctcatgtag atctgggttt tataacaatt acaaatgtaa
      121 gagttagccc tgatctaaaa ctggcaaaaa tatatttatc agtttttgaa aaagaaaaaa
      181 gagaacttgt tcttagtaaa attgaagata aaaaaggatt tataagatcc gaattggcac
      241 acaggataag aattaaattt atacctgagc tgaaattttt tatagatgat actttagatt
      301 acgttgaaaa gattgaagga ttgattaaga aaatacacga agatgataac aaaacagacg
      361 aaggatcccc ggactcttga ttttttagca gggcaggtta ttcttataga taaacctgtt
      421 aactggacat cgtttaatgt tgtaagtcgt gttagaaggt taacaaaagt taaaaaagtg
      481 gggcatgcag gaactcttga tccgcttgca accgggttgt tgattgtttg cacagggaaa
      541 aaaacaaaag agatttctga ataccaggca caaagaaaaa aatataccgg gataattact
      601 ctcggtaaat cttcgccatc aatggatttg gcaactgaaa ttacagaaag tgtattgcca
      661 gctgatttga acgaagaaaa aattctttct gccagaaata agttcttagg aaatattgaa
      721 caagttccgc caatgttttc ggcattaaag cataaaggca aaacacttta taaacttgca
      781 agagccggaa aagaagttga tcgcgcttcc aggcaagttg aaatttattc gtttgatatt
      841 acgaggattg atttaccgga tatacatttt gaaatatcct gttctaaagg aacttacata
      901 agagttattg ctgatgattt tggaaaagaa cttggttgtg gcggcgtact gagcttgctg
      961 agacgaactg aaattggtga ttataaagtt gatgatgctt ttgatttgga cgagtttaca
     1021 gcagagttta gcgaagtaca gagtagttga ttattattta tgcaatactt ttgtcatccc
     1081 gagcgaagtc gggagatgaa actaaaaaat tatttgtaac ataattctta gatctaaata
     1141 ggtacaacaa ggtttttatt aacagttatc gtgatgaaat tatagatgca ggtttttaat
     1201 agtattaaag atttaactaa gaataaaaat actgttctta cacttggcac atttgatgga
     1261 attcatcccg gtcatttgaa aattattgat aagctggttg acagttcgaa ggaaaaaagt
     1321 tatagaaatg ttgttatcac attttatcct catccaagaa cagtattaag tcctggtggt
     1381 gaagttaaaa tgttaacaac acaggaagag agaaaatatc ttcttgaaaa acatggagtt
     1441 gaaatttttt taacaataaa ttttacaaaa gaattcgcat cgcttactgc tgaagaattt
     1501 atttatgatt atctgataaa cggaatcggc ttaagtgaaa ttgttttggg acatgatcat
     1561 cattttggga aaggcagaac aggaaatgtt gagcttctaa aaaaaatagg agcaaaagaa
     1621 aatttttctg ttacaactgt cgatgctttt tatatcaatg atgtggttgt aagcagtaca
     1681 aagataagaa atgcactgaa tgacggcgat cttaaaaaag ctaatattct gcttggaagg
     1741 aattattcat tcagcggaac agtaattggc ggagataagc ggggaagaga gcttggttat
     1801 ccgacagcaa atataaaatt atctgcgcag gaaaaacttc ttcctgcaat tggtatttat
     1861 gctgttagag ttcttttaga aaatgaatat cattttggac ttttaagcat tggtaaacgc
     1921 ccaacatttt ataatgaagg tgatcttgtt actgaggttt atctttacaa tttcaatcgt
     1981 gaaatttatg gtgaacttgt aacaattgaa ttggttgaac gtcttcgtgg tgaagaaaaa
     2041 tttaattctg ctgaagaatt aatcaaccag atgaataaag acaaagaaaa cggacttaaa
     2101 atttttaatg aactaaataa ttaattccgg ttaactctgg aattatatcg tacaaataaa
     2161 taaaggatag ataaaatgac aaaagaagaa aaattagaga tcatctctaa atacggaaaa
     2221 gattcaaacg attcaggcaa gtctgaagtt cagattgctt tactaacaaa aagaatcaat
     2281 gatcttacct cacattttga tgctcacaaa aaagatcatc attcaagaag aggattaatg
     2341 atgatggttg gtaagagaag aagacttctc gattatttaa taaaaaaaga tattgcacga
     2401 tacagagcaa taattaaaga tttgaacatc agaaagtaaa ggtttagtaa tggttgttaa
     2461 gaaagaagtt gaaataggcg gaagagtatt ttcgatagag acaggaaaat atgctaaaca
     2521 ggctaacgga tctgttatgg tgcgctacgg tgatactatg gtgctggtta ctgcagttgc
     2581 tgcaagtgaa gctaaggaag gtctagatta ttttccgctt caggttgaat accgcgaaaa
     2641 aacatctgcc gcaggaaaat ttcccggtgg atatattaaa cgcgaaggca gaccaactga
     2701 aaaagaaatt ctcagctcaa gattatgtga taggcctata agaccattgt tccctgcgag
     2761 ttttacaaat gaaactcagg tcgttgcgat ggtaatgtct tatgatggcg aaaatgatgc
     2821 cgatgtattg gctgcatgcg gagcctcagc agcacttgcc atttcagata ttcctttcga
     2881 tggcccgatg gctgaagtta gagttggaag aattaatggt ggacttatcg ttaacccgac
     2941 tcacgagcaa attaagttag gtgatatcga acttgttgtt gcaggcacag cagactccat
     3001 tatgatggtt gaaggcgaat ctaaagaggt gagcgagcag gaattgcttg atgccttaaa
     3061 atttgctcag caggaaatca aaaaaattgt tgaacttcaa aatcaattag tagcagaagc
     3121 tggaaaaact aaatggactg ttgaagaaaa aacagttgat gcaaatctta aaaaagaagt
     3181 ttatgattta gctgaagcaa agtttaaaga aatcgtttat tcaattcttg ctaaagaaga
     3241 aagagcttca aagaataaag aactttccga atctgtaaaa caagcacttg ctgaaaaata
     3301 tcctgagcaa gaaaaagtta tcggtgaaat tcttcacgat atggaaaaag atcttatgcg
     3361 tgaaagaatt ttaaaggaag gtgttcgcct tgatggaaga aatactacac aggttagacc
//
LOCUS       DASWBY010000520         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_735474, whole genome shotgun sequence.
ACCESSION   DASWBY010000520 DASWBY010000000
VERSION     DASWBY010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735474"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF870_09365"
     CDS             complement(<1..228)
                     /locus_tag="VF870_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252440.1"
                     /translation="MENLKVYINYIIIVLVTAVSFSYAQDSSKIIILSDGIGLLVDSL
                     ERKEYEILPIFEDNFISAVFYLGSDNQYYCKV"
     gene            complement(440..847)
                     /locus_tag="VF870_09370"
     CDS             complement(440..847)
                     /locus_tag="VF870_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252441.1"
                     /translation="MNDDLWNWLPGKVNLENRYFELKFGSDPKPVARIGVGENKNFVV
                     EFLLNINYDIEKLKEIFSEVLWEIELYLINNSEPDPIEYMINHTKKCANMYSKVHWRY
                     YPKGFKHPILLGKENSFKESINPFKKLFLRLID"
     gene            complement(840..>1587)
                     /locus_tag="VF870_09375"
     CDS             complement(840..>1587)
                     /locus_tag="VF870_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004571 - mannosyl-oligosaccharide
                     1,2-alpha-mannosidase activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HEX9252442.1"
                     /translation="EVYKRRSKIGLVGTTINVETGEWQNTETHISGMIDSYYEYLIKS
                     YLLFGDVDFKMMYEESIAAVNKYLLDSVSTGVWCAHADMNSGVKTSTVFGALDAFMPA
                     MLVLGDDLQTAERVQESCYRMWTHFGIEPEEFDYRTFEVTSPYYILRPENIESAFYLY
                     RATKNSKYLKMGKTFFDSITKYCKIDEGFASLKNIMSKEKMDSMESFYLAETLKYLYL
                     IFAPEDILDLNKYVFNTEAHPLKKLSNQNE"
BASE COUNT          517 a          274 c          215 g          581 t
ORIGIN      
        1 tactttacaa taatattgat tatcagaacc tagataaaaa acagcactta taaaattatc
       61 ttcaaatatc gggaggattt catattcctt tctctctaat gagtcaacaa gtagtccaat
      121 accatcgcta agaataatta tttttgaact atcttgtgca tagctaaaag aaactgctgt
      181 tactaaaact attataatat agtttatata aacctttaag ttttccataa gtgaccttct
      241 gaaaaattta attgcatcag aactcttatt tataatatta aatattcact ttggagttgt
      301 catgaaattg tcaaaaatgt gtgggataat tatttgttgt caattaaaaa ttataaacaa
      361 agcgagatta tttcttgcct tttaataatg atcacaatat ctaattgtcg aataaatttg
      421 caggatttaa gactaatgat tagtctatca gccttaaaaa cagttttttg aatgggttaa
      481 ttgattcttt aaaggaattt tcttttccca gtaagattgg atgtttgaag cctttgggat
      541 agtatctcca atgaactttg gaatacatat ttgcacattt ttttgtgtga tttatcatat
      601 attcgatagg atcaggctcg ctgttattaa ttaaataaag ctcaatttcc cacagcacct
      661 cactaaaaat ttcttttagt ttttctatat catagtttat atttaataag aactcaacaa
      721 caaagttttt attttctccc actccaattc ttgcaactgg tttggggtcc gaaccgaatt
      781 ttaattcaaa gtatctattt tcaagattaa ctttaccagg cagccagttc cataaatcgt
      841 cattcatttt gattgctcaa tttttttaat ggatgtgctt ctgtattaaa tacatatttg
      901 tttaaatcca gaatatcttc cggggcaaaa atcagataaa gatactttaa tgtttcagca
      961 agataaaaac tttccatcga atccattttt tctttgctca taatattttt taaggatgca
     1021 aatccttcat caatcttgca atactttgta atactatcaa aaaaagtttt tcccattttt
     1081 aaatattttg aattttttgt tgcccgataa aggtaaaaag ctgattcaat attttctggc
     1141 cgtaaaatat aataaggtga ggttacttca aaagttctat aatcaaattc ttccggctca
     1201 attccaaagt gtgtccacat tctataacaa ctttcctgaa ctctttcagc tgtttgcagg
     1261 tcatctccaa ggacaagcat agcaggcata aaagcatcaa gagcaccaaa aacagtgcta
     1321 gtctttactc ccgagttcat atcagcatga gcacaccaaa ctcctgtgct tacagaatcg
     1381 aggagatatt tatttactgc tgcgatgctt tcttcataca tcattttaaa atccacatca
     1441 ccaaagagca aatatgattt tataagatat tcataatacg agtcaatcat cccactgatg
     1501 tgggtttctg tattttgcca ttcaccggtt tctacgttta ttgttgtacc aaccagtcca
     1561 attttagatc ttcgtttgta aacttct
//
LOCUS       DASWBY010000521         4400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_699307, whole genome shotgun sequence.
ACCESSION   DASWBY010000521 DASWBY010000000
VERSION     DASWBY010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699307"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..163
                     /locus_tag="VF870_09380"
     tRNA            91..163
                     /locus_tag="VF870_09380"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Phe,seq:gaa)
     gene            423..2111
                     /locus_tag="VF870_09385"
     CDS             423..2111
                     /locus_tag="VF870_09385"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX9252443.1"
                     /translation="MPDLITRKEFIKKSSGALIAVGLIGSASAIISSCSRPSLDILIK
                     NGNIIDGTGKGEFPADIGIRDGKIVSIENYGILKDDNSIRIIDANNLKVTPGFIDIHS
                     HTDTGLFINPNAESKIRQGVTTEVTGQDGSSVAPRKNNSGDDDEYEVENKRWTTFPEF
                     FKLLEENKSAVNLATFVGQGTLRGFVVGQDDRIATSEEIEQMKKLAMEALDQGAFGIS
                     SGLEYTPGSFASTEEISELCKVMKNRGGIYATHMRNEADTVLEAITEAIQIGKAAGVP
                     VNISHLKLQGKANWNKIDQALALIENANNNGYKVTFDRYPYTAFSTGLSNLFPLWSRD
                     GGRERFLERLQNKKDLIEIKKYVSDKVASLSSWGAVLIAGVKKENNKQYEGKTIQQIV
                     SETNEDEFEFTRKLLIDENGTVSMCGFGMSEENTSRILAHPLCMVASDASARATYGKL
                     SEGNPHPRSYGTFPRFLGTYVRQNNIVTLPEAIRKITSFPAETLGISDRGIIAKGKMA
                     DVVCFDYNNIIDKSDYVNPHQYSQGVKYVVVNGELVIDKGEHTGKLPGQILRKS"
     gene            complement(2215..2667)
                     /locus_tag="VF870_09390"
     CDS             complement(2215..2667)
                     /locus_tag="VF870_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252444.1"
                     /translation="MKYILALIMLISAMLITTVNAQDQSTRYEEQHHSKFIKSHLPQT
                     EEMLLKNFTSNDAAMLSSNIQTLRELEEVFPENKFSSLIDPLMKIVQDEKQGTQVRIL
                     AALALDDLHSDKGDKSIYGTAKSSKNQSVKDICAALAVESFKFDTAAK"
     gene            complement(3129..3377)
                     /locus_tag="VF870_09395"
     CDS             complement(3129..3377)
                     /locus_tag="VF870_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252445.1"
                     /translation="MKTKIFENAIKNRNRISFLYGLNEVSIEPYYMTFEKNGSKVIYG
                     KVYKLNEIQKFDFSKIANIKILEDDRFAPVIPFVSYVQ"
     gene            complement(3729..>4400)
                     /locus_tag="VF870_09400"
     CDS             complement(3729..>4400)
                     /locus_tag="VF870_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252446.1"
                     /translation="MRSLQKVLGIILLSIFTFSACNENVSDPLTPVSNASKSLAKQVD
                     VQKYQRIAKVMAANNIGQGAYFMEPTDAGYRLGLAKDVVIDWSTFTFISGEFAEFAGS
                     YGKGDFWRQNPDGTVSVKLTTNQAIADYFNIETGEYYAGTGNMNTKFTYTSEEYCYYD
                     EVLDSTFCFTFLFEDPSNNAWVIHGNAALTLDGAGGNSRMLQMWWNINPGGGWENPHI
                     DFNLK"
BASE COUNT         1457 a          704 c          790 g         1449 t
ORIGIN      
        1 tggctaagag tgtaaccgtt gaataattat tttcaatctg tttttgtgat ttgctggaaa
       61 atctatattt ttacgcacta aattttagaa gggcaggtag ctcagtcggt agagcaaagg
      121 actgaaaatc cttgtgtcgg cggttcaatt ccgtccctgc ccacaccctt taacccgcta
      181 accgaagcgg gttttttgtt tttaaatgta aaaacccacc aacttttgaa ttatttgatt
      241 aatttattta tttatttgat tgaattgcaa gaaataggta agatagcttg taaacttttg
      301 taccggttta aaataactga taaatatcaa aacaaaatac ctattgttaa ttgcgatttt
      361 tttatttttc aaactgataa tttctattaa gataactttc caaaatattt tctggagtag
      421 taatgcctga tttaatcacg agaaaagaat ttattaagaa aagctctgga gcattaattg
      481 ctgttggttt aatcggcagt gcgtctgcaa taatcagtag ttgcagcaga ccttctttag
      541 atattcttat taaaaatggt aatataatcg acggaaccgg caaaggtgaa tttcctgctg
      601 acattgggat aagagatgga aaaatcgttt cgattgaaaa ttatggaatt ttaaaagatg
      661 ataattcaat aagaattatt gatgcgaata acttaaaagt tactcccggt tttattgaca
      721 ttcattccca tacagatacc ggattgttca ttaaccccaa cgctgaaagt aaaattagac
      781 aaggtgttac tacagaagta acagggcaag atggaagttc cgttgctcca aggaaaaata
      841 attccggtga tgacgatgaa tatgaggtgg aaaataaaag atggacgacc tttcctgaat
      901 ttttcaaatt acttgaggaa aataaatcag ctgtaaattt agctacattt gtcggccagg
      961 gaacattgcg aggatttgtt gtaggacagg atgacaggat agccacctct gaagaaattg
     1021 aacaaatgaa aaaacttgcg atggaagcat tagatcaggg tgcttttggg atttcttccg
     1081 gattagaata tacaccaggt agttttgcaa gcaccgaaga aatttcggaa ctgtgtaagg
     1141 ttatgaagaa tcggggaggg atatatgcaa ctcacatgag gaatgaagct gatacagtat
     1201 tagaagctat tacagaagca atacagatag gaaaagcggc aggcgttccg gttaacatct
     1261 ctcatctaaa attgcaggga aaagcaaact ggaataaaat agatcaggct ttggctttga
     1321 ttgaaaatgc aaataataac ggatataaag taacgttcga taggtatccc tacactgctt
     1381 ttagtaccgg tctatccaat ctatttcctt tatggagcag ggatggggga agagaacgat
     1441 ttttagaaag acttcagaat aaaaaggatc ttatagaaat aaaaaaatat gtgtcagata
     1501 aagtggcttc gcttagttcc tggggtgctg tcttaattgc tggggttaag aaagaaaata
     1561 ataaacaata tgaaggcaaa actattcagc agattgtatc ggaaaccaat gaagatgagt
     1621 ttgagtttac acgaaaactt ttgatcgatg aaaatggaac cgtctcaatg tgtggatttg
     1681 gtatgagtga agaaaatacg agtagaatat tagcacatcc actctgcatg gtagcttctg
     1741 acgcttcagc aagagcaaca tacggtaagc tttcagaagg taatccccat ccacggagct
     1801 atggaacctt cccaagattt ttgggtacat atgttagaca aaacaatatt gttactttac
     1861 ctgaagcaat aaggaagatc acttcgtttc cagcggaaac tcttggtatt tcagaccgag
     1921 gtattatagc taagggaaag atggctgatg ttgtttgctt cgattataat aatattattg
     1981 ataaatcaga ttatgttaat cctcatcaat attcacaagg tgtgaaatat gttgtggtga
     2041 atggtgaatt agttattgat aaaggcgagc atacaggaaa attacctggt cagattttga
     2101 ggaagagtta attggattaa taaatcaaca gtgtttatta aattataact tttaggatgt
     2161 atataaaaaa gaccgtcata gagacggtct tttattttta tttaataatc gactttattt
     2221 tgcagccgta tcaaatttaa agctttctac tgcaagcgca gcacaaatat ccttcaccga
     2281 ttggtttttt gaggacttag cggtaccgta aattgattta tcacctttat ccgaatgcag
     2341 atcgtccaaa gcaagtgctg ctaaaattcg aacttgtgtc ccctgtttct catcctgcac
     2401 gattttcatc aatggatcga tcaaagaact gaatttattc tctgggaata cctcctccag
     2461 ttctctaaga gtttgtatat tcgaagaaag cattgcagcg tcattgctag tgaaattctt
     2521 taaaagcatt tcttcagttt gcggtaaatg tgatttaata aatttggaat ggtgttgttc
     2581 ttcatagcgg gtagattgat cctgggcatt aactgttgtt ataagcattg ccgatataag
     2641 cataattaga gccaatatgt atttcataat ttattccttt aataattttt ttaatatcaa
     2701 tcagttaatt ataagttgat ttctcttcag cctgacttta attactaaag agaaaatttg
     2761 atgtgaataa aaaatttttc tttatagctc cttcgtttta attttaatga aaaaagacat
     2821 ttattattaa attaaatttc aaatactaac ataatcccat aataggttgt cacttaatta
     2881 agaatgttta gtttgtttgc aaagatactt tttccatttg tgtaagtgtt aggacaatat
     2941 cagcattcta caagattttt attgcattta ttgttttatt cagcttctaa gattaattca
     3001 gcttccattt aataatggaa tagaaggtaa attatattta gatatcttaa tgaaaatccc
     3061 ctataaaaaa tcatcagagt ttgtgataac atttactaat agccaatttt tcaataaaca
     3121 cttttctttt actgcacata tgaaacaaat gggataacag gcgcaaacct atcatcttca
     3181 agtattttta tgtttgcaat tttactaaaa tcgaattttt gtatttcatt taacttatag
     3241 actttcccat aaatgacttt tgatccgttt ttttcaaaag tcatataata aggttctatc
     3301 gatacttcat ttaatccata cagaaaactt atacgatttc tattctttat tgcattctca
     3361 aatattttcg ttttcatatc ttaatccttt tttattgtga tatttaattc tttatcaaga
     3421 taaagacaca ctatatgctg tttaatagca aatatctttt gaagccatca aaaattcaca
     3481 gcataacctt aatcaaataa cattaattaa aaattaaaat tgtattaaaa aaaattaatc
     3541 tatcacttga ttacttagct taacaactta agaacattac ttaggttttt ggtttaggtg
     3601 ataaaaaata taggggtaga ctattgaaga tggagcgcca gtagcagcag cgtaaatgaa
     3661 aaaaacgagt cagtgtttta agccgacccg attttacaat agttaattat atctgaaaag
     3721 agttgtggtt attttagatt aaaatctatg tgtggatttt cccaccctcc acctggatta
     3781 atattccacc acatttgcaa catgcgtgaa tttcctcctg caccgtccaa agtaagagca
     3841 gcatttccgt gaattaccca agcattattg ctgggatcct caaagaggaa agtgaaacag
     3901 aacgtcgagt caaggacttc atcataataa caatattctt cacttgtata tgtgaatttt
     3961 gtattcatat tccctgtgcc agcgtaatac tctcctgttt caatgttaaa gtagtctgca
     4021 atagcctggt tagtcgttaa tttgactgaa acagtcccat ctggattctg gcgccaaaaa
     4081 tcgccttttc cataactacc tgcaaattct gcaaactcac cactgataaa tgtgaacgta
     4141 gaccagtcga ttaccacatc cttagcaagc cccaaacgat aaccagcgtc tgttggttcc
     4201 atgaagtagg cgccttggcc tatattattc gccgccataa cctttgctat acgttgatac
     4261 ttttgaacgt ctacttgttt tgctaatgat ttactagcat tgctaacagg tgttagtgga
     4321 tcagagacat tctcattaca agcagagaat gtaaagatgg ataataaaat tatcccgaga
     4381 actttttgga gacttcgcat
//
LOCUS       DASWBY010000522         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_320081, whole genome shotgun sequence.
ACCESSION   DASWBY010000522 DASWBY010000000
VERSION     DASWBY010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320081"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VF870_09405"
     CDS             <1..608
                     /locus_tag="VF870_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX9252447.1"
                     /translation="IGAMIPFLFSALAMQAVGKAAMAMIEEVRRQFNTIPQLKAALGV
                     MKKNDGIEHSAWSKEDLGIFEDAEGKAEYGKCVEISTKSAIKQMVLPGLLAVVIPILA
                     GFLGGKEMLGGLIAGVTVSGVLMAIFQANAGGAWDNAKKMFEEGIEVNGQKYYKGSDA
                     HKAAVVGDTVGDPFKDTSGPSLNILLKLMSVVALVIAPLIR"
     gene            706..1128
                     /gene="rpsF"
                     /locus_tag="VF870_09410"
     CDS             706..1128
                     /gene="rpsF"
                     /locus_tag="VF870_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX9252448.1"
                     /translation="MRTGVYESAVLINAALDDQQIDAILTRIKDLITNNGGQIREIDN
                     WGRKRLAYPVEKSKIGYYAIFRFDAPSDIVAKLERTYTLDEQILRFVTLKLSSDALEQ
                     LEKNKTLSSTIKDELIPDTVEIIAPVAEADTESEDKGK"
     gene            1160..1666
                     /locus_tag="VF870_09415"
     CDS             1160..1666
                     /locus_tag="VF870_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9252449.1"
                     /translation="MADLKMPEINYVIVAGNLTKDPIFRETTNGTPVVNFSIASNRKF
                     KDSTNQWQEDVCYVGVVAWNKLAESCRDRLRKGSAVLVDGELQSRSWKSDDGHNRSIV
                     EIKARRIQFLNKRLVGSDGNGSVEEDDISVAVDESIDYTEDSFDKFLSEEESSLIKQS
                     GNGNEERS"
     gene            1650..1880
                     /gene="rpsR"
                     /locus_tag="VF870_09420"
     CDS             1650..1880
                     /gene="rpsR"
                     /locus_tag="VF870_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX9252450.1"
                     /translation="MKKEVKTKKVCRFTQNGIKYIDYKDVKLLQKFITEQGKIIPKRI
                     TGTSSKYQRELASAIKRARHMALLPYVSDTVR"
     gene            1903..2346
                     /gene="rplI"
                     /locus_tag="VF870_09425"
     CDS             1903..2346
                     /gene="rplI"
                     /locus_tag="VF870_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9252451.1"
                     /translation="MKVILRQDFETLGKIGEVVDVKDGYARNFLFPRGLAYAALKGNI
                     RALDEEKKSVEKRNLQELKAAESLAAELETVSVTIPVQVGEEDKIFGTVTTQMIADAL
                     KEKGHDIDKRKVEIEEPIKALGIYAVNLKIHQNVNAKIKVWVVRE"
     gene            complement(2419..2658)
                     /gene="rpmB"
                     /locus_tag="VF870_09430"
     CDS             complement(2419..2658)
                     /gene="rpmB"
                     /locus_tag="VF870_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX9252452.1"
                     /translation="MARRCQVTGVSPVSGSNISHAHNHTKTRFLPNLQKKRIWVQELN
                     RFVTIKLTTSAIKTISKKGTAHIAKLISDKKIKVR"
     gene            2876..3487
                     /locus_tag="VF870_09435"
     CDS             2876..3487
                     /locus_tag="VF870_09435"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX9252453.1"
                     /translation="MEFFELHPQNPQIRFISKAVDVLKSGGIIIFPTDTYYGLGCDLF
                     NKEGIEQILSIKQENDTKLFSFICSDLKDISKYAKVSDYAYRTMKHLLPGPYTFILPA
                     AKVIPKKLWSKRKTVGIRVPDHAVTLQLVRELGNPIASTSTTTRLGEPLLDPFEIKNI
                     FNSRVDLMLASNTMSFEPSSVVDLSGEVPEIIREGAGDISLFK"
     gene            complement(3527..>3931)
                     /locus_tag="VF870_09440"
     CDS             complement(3527..>3931)
                     /locus_tag="VF870_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9252454.1"
                     /translation="TRVVSPGMVERQSGHIINIGSTAGHEVYTYGNVYSATKFAVKSL
                     TQSVRLDMLDKGIKVSSVDPGMVYTEFSKVRFSGDDLRAEQVYKGITPLSPTDVAEAI
                     VFCATRPYNVNINEVVLTPIQQASSTQVYRKQ"
BASE COUNT         1345 a          593 c          763 g         1230 t
ORIGIN      
        1 taataggtgc aatgattccc tttttattct ctgctcttgc tatgcaggca gttggtaaag
       61 cagcaatggc aatgattgag gaagttagaa gacaattcaa cacaatccca caattaaaag
      121 ctgcactagg agttatgaaa aagaacgatg gaatcgaaca ttcagcgtgg tctaaagagg
      181 atcttgggat ttttgaagat gcggaaggga aggcagaata cggaaaatgt gttgaaattt
      241 caaccaaatc agcaataaag caaatggttc ttccaggact gctagcagta gttattccaa
      301 tattagcagg atttcttggc ggtaaagaaa tgttaggtgg tttaattgcc ggtgtaactg
      361 tttccggcgt gcttatggca atttttcaag caaatgcagg tggtgcctgg gataacgcca
      421 agaagatgtt tgaggaaggt atagaagtta acgggcagaa atactataaa ggctcagatg
      481 cccataaagc cgctgtagtt ggtgatacag ttggtgatcc atttaaggat acttcaggac
      541 catcactaaa tattttgtta aaattaatgt ctgttgttgc acttgtaatt gcacctttaa
      601 ttaggtaatt tttcagcgct ttttaataca tattttataa accctgctct tacattcatt
      661 aatgaatagt aagggcctta aagcccatag ggaggtgata caaaaatgag aacaggtgtc
      721 tacgagagtg ccgttctaat aaatgctgcg ttagatgatc agcaaatcga tgcaattctt
      781 actagaatca aagatcttat aactaacaac ggcggacaaa tccgtgaaat agataactgg
      841 ggacgtaagc gtcttgctta tccggtggag aaaagtaaaa ttggctatta tgccatattt
      901 agatttgatg cccctagtga tatcgttgca aagttagaaa gaacttatac tttagatgaa
      961 caaattttaa gatttgttac gctcaaactt agcagtgatg ctttagaaca gcttgaaaaa
     1021 aataaaaccc tttcatctac aattaaagat gaattaatcc cagatactgt tgagataatt
     1081 gctccggtag ctgaggcaga tactgaaagt gaagacaaag gtaaataatt acttaatgat
     1141 ttagtatgga ggttcacaaa tggccgattt gaaaatgccg gaaattaatt atgtaattgt
     1201 agcaggaaat cttactaagg atcctatttt tagagaaact acaaatggta ctccagttgt
     1261 taatttttct attgcttcaa acagaaaatt taaagacagc acaaatcagt ggcaggaaga
     1321 tgtttgttat gttggagttg ttgcttggaa taaacttgct gaaagctgca gagacagact
     1381 aagaaaagga tcagcagttt tagttgatgg agagttacaa agcagaagct ggaaatcaga
     1441 tgacggacat aacagaagta tagttgaaat aaaagctaga agaattcaat ttcttaataa
     1501 gcgattggtt ggatctgacg gtaatggttc tgttgaagaa gacgatatta gcgttgctgt
     1561 tgatgagtca atcgattata cagaggattc ttttgataag tttttatctg aagaagaatc
     1621 aagtttaatt aaacaatcag gtaatggaaa tgaagaaaga agttaaaaca aaaaaagtct
     1681 gtaggtttac acaaaacggt atcaagtata tcgattataa agatgttaaa cttttacaga
     1741 aatttatcac cgagcaggga aagataattc ctaaaagaat aaccggtacc agctcaaagt
     1801 atcagcgtga attagcttcg gctattaaac gtgcaagaca tatggcatta ttaccttatg
     1861 tttcagatac agttagatag ttaaaataaa gccggagaaa aaatgaaagt aatcttaaga
     1921 caagactttg aaaccttagg taaaataggc gaagttgttg atgttaaaga tggttatgca
     1981 agaaactttc tttttccaag agggctggct tatgctgcac ttaaaggcaa tataagagct
     2041 cttgatgaag aaaagaaatc agttgaaaaa cgtaacttgc aggaacttaa agcagctgaa
     2101 tcgcttgctg ctgaacttga aacagtatct gtaacaatac ctgttcaggt tggtgaagaa
     2161 gataagatct ttggaaccgt aacaactcaa atgattgccg acgcacttaa ggaaaagggt
     2221 cacgatatag acaagagaaa agttgaaatc gaagagccaa ttaaagcttt aggtatttat
     2281 gctgttaatt taaaaattca tcagaatgtt aatgcaaaaa ttaaagtttg ggttgttcgg
     2341 gagtaatctc atttctgttt aaaattaaaa agggaatcca aatggattcc cttttttcat
     2401 gtctaaaagt ttttttcgtt atctaacttt aatcttttta tcgctaataa gttttgcaat
     2461 atgtgcagtg ccttttttac ttatagtttt aatagcactt gtagttaatt ttattgtaac
     2521 aaatctattc aactcctgaa cccaaattct cttcttctga aggttgggta aaaatctggt
     2581 ttttgtatga ttgtgtgcat gcgaaatatt gcttccggaa accggactta cacctgttac
     2641 ttggcatctt ctagccattt ttatagctcc ttaaaataaa tacttttcga tttttagaac
     2701 agtaaatata agtaaatcat tacaaaaaac taaaaaaaat attataaatt tttcaaaata
     2761 tcttattaaa taaaataatt aagtctggca acagtttatc tacaattagt cttgattcta
     2821 atattattaa gggtattttt gcattaaatt tttgaaattg cttaaagtaa gattaatgga
     2881 attttttgaa ttacatcctc aaaatccgca aattaggttc attagcaaag ctgtagatgt
     2941 tttgaaaagt ggcggaatta taatttttcc tactgacacg tactatggac ttggctgtga
     3001 tttgttcaat aaggagggaa ttgagcaaat actgtctatc aaacaggaaa atgacacaaa
     3061 gctttttagt tttatttgct ctgatttaaa agatatctcc aaatacgcta aagtttctga
     3121 ttatgcatat cgaacaatga agcatctgtt accaggtcct tatacattca tattgccggc
     3181 ggcaaaagtg atcccgaaaa aactttggag taaaagaaaa actgttggta taagagtacc
     3241 ggatcatgct gtaactctgc aactagttag ggaactaggt aatcctattg caagcacgag
     3301 cacaaccaca cgacttggtg aaccattact tgatccgttc gaaattaaaa atatttttaa
     3361 ttcacgtgta gatctaatgc ttgcttctaa tactatgagt tttgaacctt cgagtgttgt
     3421 tgatttaagt ggtgaagtac ccgaaataat tcgcgaaggt gcaggcgata ttagtctatt
     3481 taaataattt tatttttaag taataaatag agaataaaac acctatttat tgttttctat
     3541 aaacctgtgt tgatgatgct tgctgaatag gagtaagaac aacttcattt atatttacat
     3601 tataaggacg tgttgcacag aatactattg cttcagcaac atcagttggg gataaaggtg
     3661 taattccttt ataaacttgc tctgctctta agtcatcacc agaaaatctt actttactaa
     3721 attctgtata gaccattccc ggatcaacac tgctgacctt tattccttta tccagcatat
     3781 ccaacctaac agattgggtt aaagatttaa cagcaaattt agttgcggaa tacacgttgc
     3841 cataagtata gacttcatgt cctgcagtag agccaatgtt gataatatgt ccgctttgtc
     3901 tttctaccat acctggagat accactcttg t
//
LOCUS       DASWBY010000523         6431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_699328, whole genome shotgun sequence.
ACCESSION   DASWBY010000523 DASWBY010000000
VERSION     DASWBY010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699328"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VF870_09445"
     CDS             <1..924
                     /locus_tag="VF870_09445"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HEX9252455.1"
                     /translation="NFGIQGGFRAEYTDRTITLLDSTEEFKIERWDYIPTLHTSYKFS
                     EGTQMMASYSRRIQRPDGWELEPFLTWIDANNVRIGNPDLLPELIDSYEAGIQTYIGE
                     VSLSAELYHRVTNYKIDRVRSVYPGADNVSLNTVVNIGKDFSTGSEIMILFDPLEFWN
                     VNLMANLYNYRIEGTLYNDSFSRESFNWSTRLNNMFKITPNTQLQVNFNYNSPGVSSQ
                     GTSEGYFTTDLSVKQDFFERLLSLTLQVRDLFGTAKHEFSSSGPDFYSYNYFKRESPM
                     VILNARINLNNFKPKRDNRDLENQNGNGEEF"
     gene            1033..2322
                     /locus_tag="VF870_09450"
     CDS             1033..2322
                     /locus_tag="VF870_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9252456.1"
                     /translation="MSFEKLKLIKPIHTALAEEGYKIPTPIQRQAIPVILDRRDLLGC
                     AQTGTGKTAAFAIPILQILSEDKEEQKSKKVIKSLIVTPTRELAIQIGESFDAYGRHT
                     GLTNAVIYGGVSQKAQTDKLMHGVDILIATPGRLLDLMQQKFVNLHHIKLFVLDEADR
                     MLDMGFIHDVHMILSKLPVKRQSLFFSATMPPEIIKLANTILTEPVSIDITPEFKTVE
                     AIKQAVYFVSKSDKRKLLYHILRNEKISSALVFTRTKHGANLVCKHLTASKISAEVIH
                     SGKSQNARQRALSNFKSLQTKVLVATDIAARGIDIDKLSHVFNFDIPNIPETYVHRIG
                     RTGRAGLGGIALSFCDEEERVYLKDIIKLIKIQIPVIHDHPYKLSKEGVQKTEQPNTK
                     SVLPEKSNTQKKSGSSGYFKNRKKRWGRKPNTNKAKT"
     gene            complement(2366..3244)
                     /locus_tag="VF870_09455"
     CDS             complement(2366..3244)
                     /locus_tag="VF870_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9252457.1"
                     /translation="MTWFIVAFISALLSAFAAITQKKVLFNLGALEFSFLLSIVNLVF
                     SIPFFFFIDYQTINTLNLSILFAKSVIGVAAFLCVMISLKNLEISNALPLLALTPGFV
                     AVFAFLLLGESLKMIEVIGLVFLIVGTFILESKNIKAFSFPFKIFSKSKYHRFVIIAL
                     FLFTASSILDKLLLIKLNLSPISLTAFQHIYFAIIFSLIYLIFKQKSEVSSVSIKKDN
                     LGWIALISILTIGYRYTQIVAVSLASVALVLAIKRTSVFWATVIGGKLFNDKDLLKRS
                     LAAILILIGAILILRE"
     gene            complement(3275..5644)
                     /locus_tag="VF870_09460"
     CDS             complement(3275..5644)
                     /locus_tag="VF870_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9252458.1"
                     /translation="MKTVILLLTIFLIQFLTFAQSIDIKIKLNNSESDAPITNANISL
                     SDKYFGTTNKDGECFLKNVNKGIYVLSISHISYYTYEKDIELLSDTTISVRLLAGKIE
                     LQDVVVTSGKYEQNIQMLPYSVSAINKKEIEMSPSFTVSDLLKTESGISLLRDGIWGT
                     EVSIRGLSRSNVVTLIDGNRIETSTDISARLSMIDLNDVDRIEVIKGASSSLYGSGAT
                     GGTINIISKSGSYTNKLTLRGSYSGGFNSVNNFYYNGLNLIASGNEWIAKFSGNYRKA
                     NNTQTPSGELDNSQFEGNSFSALLQIKPFEDQEIKLNYQQFNAYDVGIPGGAPLFPNN
                     AKVTYPEELRRLISAEYKINNLSKALIKLSAKYFHQFISRDVENIPNQVQFVPGANGQ
                     TPKRVSVLKINPSADHNTDGFQTQADLKFSNHYLIAGFDFWSRNYNGLRTRDQKIEIL
                     NSADSSVIRTMYKTIYEKPLPDAVYSSAGIYLQDEMNMFSNLNLTLGGRYDYIWLSNA
                     ETYNPLYEVNDGVVNENPAGQKIIWEAESANNKSYVFNIGLLYSLSDHSNISFNAARS
                     FRSPSLEERYQYIDLGSTIRVGDPNLKPEQGYFFDLGFRFFQNELNFISSIFLNSLKD
                     LVTEVPGTYEGRNALIKTNIGEALLYGFEYSIDYRIINQLNVYNTLSYVRGLNQKDDQ
                     DLPQISPLNGIIGIEYFPLDWMIADVSAIIFSDQNKVAPGEKTTPGYATFNFKFNFIN
                     LTLGTVKTSFSVGVENFLNKEYRNHLSTNRGSITTEPGRNFFIRVNLSF"
     gene            5917..>6431
                     /locus_tag="VF870_09465"
     CDS             5917..>6431
                     /locus_tag="VF870_09465"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX9252459.1"
                     /translation="MKQIFQVIFITLTSISFSFSQNMIFKSLTVENGLSSSDINCLIQ
                     DRLGFIWIGTDNGLNRYDGSAFKVYRNKQNNTNSVSDNSIWALYEDRNANIWIGTKGG
                     VLNKYSPVYDKFERIKLDENKDIENSITSIIEDKQGFLWIGTYSQGLYRYDPSSGKLK
                     NWKYDPKNRIG"
BASE COUNT         2263 a         1056 c         1066 g         2046 t
ORIGIN      
        1 aattttggta ttcagggtgg cttcagagca gagtataccg atagaacaat tacattactt
       61 gactcaactg aggaatttaa aatagagcga tgggattata ttcccacgct gcacacttct
      121 tacaagtttt ctgaaggcac acaaatgatg gcaagctatt ctcgtcgcat acagcgcccg
      181 gacggttggg aacttgaacc atttcttacc tggattgatg caaataatgt tagaatagga
      241 aatcccgatc tcttacctga attaatagat tcttatgagg caggaataca aacttatatt
      301 ggggaagttt cattatcagc tgaattgtat catagagtta caaattataa aattgaccgc
      361 gtgcgttcag tttatcccgg tgccgataat gtaagtctta atacagtagt taatatcggg
      421 aaagattttt ctaccggtag tgaaatcatg atactatttg atcctttaga attctggaat
      481 gtaaatttga tggctaatct ttacaattac agaattgaag gtactttata caatgattca
      541 ttttcaagag aaagctttaa ttggagcaca agacttaata atatgtttaa gataacgccg
      601 aacacacaat tacaggttaa ctttaattat aacagcccgg gcgtttcttc acaaggaact
      661 tcagaaggat attttactac tgatctttct gttaagcagg atttttttga aaggttacta
      721 tcacttaccc tgcaggtaag agatttattc ggaactgcta aacatgagtt tagttcttcc
      781 ggaccagatt tctattctta taattacttc aagcgagagt caccaatggt gatattaaat
      841 gccagaatta atcttaataa tttcaaacct aaacgggata atagagattt agagaatcag
      901 aacggtaatg gggaagagtt ttaatttgac caacaaattt ttattcttca ttttattcag
      961 taatataaat cattgatcat tactctttat tgcagtaact gttagattct cagaacatta
     1021 accaggaatt ttatgtcatt tgaaaaatta aagctaatta aacctattca tacggcactt
     1081 gcagaggaag gatacaaaat tccaacacct atccagaggc aggcaattcc agtaattctt
     1141 gatagaagag atttacttgg ttgtgctcaa accgggacag gaaagactgc agcatttgca
     1201 atcccaatac tgcaaatcct tagtgaagat aaggaagaac aaaaatctaa aaaagtaata
     1261 aaatcattaa tcgttacgcc aaccagagaa ttagcaattc aaattggtga gagttttgat
     1321 gcatatggaa gacatacagg actgacaaat gcagtaatat atggcggagt ttcacaaaag
     1381 gctcaaacag ataagttaat gcatggtgtc gatattttaa ttgcaacacc aggaagactt
     1441 ctggatttga tgcagcagaa atttgtgaat cttcatcaca ttaagctgtt tgttctggat
     1501 gaagctgata gaatgctgga tatgggattt atccatgatg tgcatatgat tttatcaaaa
     1561 ctgccggtta aaaggcaatc gctgtttttc tcagccacaa tgcctcctga aattataaag
     1621 cttgcaaata caatattgac cgaacctgtc agtattgata taacaccgga attcaaaact
     1681 gtagaagcaa ttaagcaagc cgtttatttt gtctccaaat ctgataaaag aaaattatta
     1741 tatcatattc tgagaaatga gaaaatttct tctgctcttg tttttacaag aacaaaacac
     1801 ggcgcaaatt tagtctgcaa acatttaact gcctcaaaaa tatctgcgga agtaattcat
     1861 agtggaaaat ctcaaaatgc aagacagagg gcactgagta actttaaatc gttacaaacc
     1921 aaagtactcg ttgccacaga tattgctgct cgtggaatag atatcgataa actatcacac
     1981 gtttttaatt ttgatatacc taatattcca gaaacgtatg tgcatcgtat tggcagaaca
     2041 ggcagagcag ggttgggagg aattgcactt tcattttgtg atgaagaaga gagagtatat
     2101 ttaaaggata ttattaagct tataaaaatt caaattcctg taatccatga tcatccctat
     2161 aagctaagca aagaaggcgt tcaaaaaaca gaacagccta acacaaaatc agttttacca
     2221 gaaaaatcta atacacaaaa aaaatcaggt tcatcaggtt actttaagaa tagaaagaaa
     2281 agatggggac gaaagcctaa tacaaataag gctaagacat agtttattcg actgtcattc
     2341 ccgtgtaaac gggaatctga ccgaactatt ctctcaaaat taaaattgct ccaatcaaaa
     2401 ttagtattgc tgcaaggctt ctcttcaaaa gatctttatc attaaataac tttcctccta
     2461 taacagtcgc ccaaaaaacg gaagttctct ttattgctaa tacaagtgcc actgatgcaa
     2521 gacttacagc aacaatttga gtataccgat atccgattgt taatatggaa attaaagcta
     2581 tccatcctaa attatctttt ttaatactta ctgatgacac ttcagatttt tgtttgaata
     2641 ttaaatagat taatgaaaat atgatagcaa aatatatatg ctgaaatgcg gtaagtgata
     2701 ttggtgaaag atttaactta attaataaaa gtttatccag aatagaagaa gcagtaaata
     2761 ggaaaagtgc aataataaca aaccggtggt attttgattt tgaaaatatt ttaaaaggaa
     2821 aagaaaatgc tttgatgttt ttgctttcaa gaataaatgt tccgacaatc aaaaatacca
     2881 atcctataac ttcaatcatt tttaatgatt ctccaagaag cagaaacgca aatacagcaa
     2941 caaatcccgg agttaatgct aataacggaa gtgcattgct tatttctaag ttttttagtg
     3001 atatcattac gcagaggaat gctgcaacac caattacaga cttagcaaat aaaatggaaa
     3061 gatttaaagt gtttattgtt tggtaatcaa taaaaaagaa aaaggggatt gaaaaaacaa
     3121 ggttaactat cgagagtaaa aatgaaaact ctaatgcgcc aagattaaat agaaccttct
     3181 tttgagttat tgctgcaaat gcggaaagca gtgcggagat aaaggcaaca ataaaccaag
     3241 tcataatatt ctttcttttg aatagctcta aaaattaaaa tgaaagatta actcttataa
     3301 aaaagtttct gccaggttca gttgtaattg aacccctgtt tgttgaaaga tgatttcgat
     3361 actccttatt caaaaagttt tcaactccaa cgctaaaact ggtttttacg gtacctaaag
     3421 ttagatttat aaaattaaat ttaaaattaa atgttgcata acccggagta gtcttttctc
     3481 caggtgcaac tttattctga tcggaaaata taattgctga aacatctgca atcatccaat
     3541 caagagggaa atactcaata cctataattc catttaacgg agatatttgc ggaagatcct
     3601 gatcatcttt ttgatttaat cctcgcacat aggacagtgt attataaaca tttaattggt
     3661 ttattattct ataatcgatt gaatactcaa aaccatataa taatgcttca ccaatattag
     3721 ttttaattag tgcatttctg ccctcataag ttcccggtac ctctgtaaca aggtctttca
     3781 aagaatttag aaaaatgctg gatataaaat ttaattcatt ttgaaaaaac cgaaaaccta
     3841 aatcaaagaa atatccctgc tctggtttta aattaggatc accaactctt attgtactgc
     3901 caagatcaat gtactgatat cgttcctcta gcgaaggcga acggaatgaa cgtgctgcat
     3961 taaatgaaat atttgaatga tcacttaagg agtaaagcag accaatatta aaaacataag
     4021 atttattatt tgcggattct gcttcccaaa ttattttttg tcctgcagga ttttcattta
     4081 ccactccatc atttacctcg tataatggat tatatgtctc agcattactt aaccagatgt
     4141 aatcgtacct tccgccaagt gttaggttta gattagaaaa catattcatt tcatcctgca
     4201 gataaattcc tgcactactg taaactgcat ccggcaaagg tttttcataa atagtcttgt
     4261 acatagttct aataacagaa gaatcggctg aatttaaaat ttcaattttc tggtcacgag
     4321 ttcttaatcc gttgtaattc cttgaccaaa aatcaaaacc ggcaatcaga taatgatttg
     4381 aaaattttaa atcggcctga gtttgaaatc catctgtatt gtgatctgca gaaggattaa
     4441 tttttaaaac acttactcgt tttggtgtct gaccattagc accaggaaca aattgaacct
     4501 gattcggaat attttcaaca tctcttgaaa taaactgatg aaaatattta gcagataatt
     4561 ttattaatgc tttgctcaaa ttatttattt tatattctgc acttatcaat cttctcagct
     4621 cttcaggata agttacctta gcgttatttg gaaataaagg agcgccgccc ggaatgccaa
     4681 catcataagc attaaactgc tggtagttta gttttatttc ctggtcctca aaaggtttaa
     4741 tttgaagcag cgcactgaaa ctattgcctt caaattggct gttatctaat tcacccgaag
     4801 gtgtttgtgt attatttgct tttctgtaat taccactaaa cttggcaatc cattcattac
     4861 cggatgcaat aagatttaat ccgttgtaat aaaaattatt tacagaattg aagccacccg
     4921 aataagatcc tcttaaagtt aatttgttag tatagctgcc tgatttagaa attatattta
     4981 tcgtcccgcc tgtggcacct gaaccataga gtgaagaaga tgcgcccttt ataacctcaa
     5041 ttctatctac atcattaaga tcgatcatag aaagtcgtgc acttatatca gttgaagttt
     5101 caattctgtt tccatcaatt agtgttacta cattggagcg gcttaatcct ctgatgctta
     5161 cttcggttcc ccaaattcca tcacgtaaca gcgagatacc tgattcagtt ttcaacaaat
     5221 ctgaaacagt aaatgaagga ctcatctcaa tttctttttt attgattgca gagactgaat
     5281 atggaagcat ttgaatattc tgctcatact taccagatgt aacaacaaca tcctgaagtt
     5341 ctattttacc tgctaatagt ctaacagaaa tagtcgtatc agagagtaat tcaatgtctt
     5401 tttcataagt gtaatagctg atgtgagata ttgataaaac atatataccc ttatttacat
     5461 ttttaaggaa acactcacca tctttatttg tcgtcccaaa atatttatca gataatgaaa
     5521 tatttgcatt cgtaattggc gcatcgctct ccgagttgtt taattttatt ttaatatcaa
     5581 tcgattgggc aaaggttaaa aattgaataa gaaatattgt taaaaggagg atgacggtct
     5641 tcataaaatg tctacaaaag ttaaaatatg atctgcaaat tatgctgaac taaaaattca
     5701 aaatttgatt agtaaatata actacagtgc ttgcaggtga gcaaaaaaat cgaatcaatt
     5761 attcagcttt gactaaatac agtatactgt taaaataatc tactttttgt ctaaaattat
     5821 catcatataa aagcatatat tagcgccgtt tactattaca acttgtctca attcatttac
     5881 ttatttaatt tttaccgcat atttaaaaac aataatatga agcagatctt tcaagtaata
     5941 ttcattacac taacttcaat ttcattttcc ttttcgcaga atatgatatt taaatcatta
     6001 actgttgaaa acggattatc aagtagtgat ataaactgtc taattcagga taggttaggt
     6061 tttatttgga ttggtacgga taatgggctc aatagatatg acggttctgc attcaaagtc
     6121 tatagaaata aacaaaataa taccaatagt gtttcggata atagcatttg ggcattgtat
     6181 gaagatagaa atgcaaatat ttggattgga acaaaaggcg gagtgctaaa taagtattct
     6241 cctgtatacg ataagtttga aagaattaag ctggacgaaa ataaggatat tgagaacagc
     6301 attacctcta tcattgaaga taaacaagga tttctgtgga ttggaacata ttcacagggt
     6361 ttataccgct atgatccttc aagtggtaaa ctaaaaaact ggaagtacga tcctaaaaac
     6421 agaattggat t
//
LOCUS       DASWBY010000524         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_95182, whole genome shotgun sequence.
ACCESSION   DASWBY010000524 DASWBY010000000
VERSION     DASWBY010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95182"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="VF870_09470"
     CDS             <1..1752
                     /locus_tag="VF870_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX9252460.1"
                     /translation="LVGETNTELFYQTYRNNYVRKADIDSIKSWGFNSIRLPFHYNLF
                     ATNTNPPTFINLGFEIIDSLLGWCKQNEIYLILDMHGAPGGQSAEPISDYNPANPSLW
                     ESESNKTLTVQIWRKIAERYKDEVWIGGYDILNEPAWNLPQNNQPLRDLYIRITDTIR
                     AVDNNHLLFIEGNWFATDFNGLTPAWDENMAYSFHKYWNGNTQSSIQYLVDLRNNTNR
                     PLWLGETGENSNKWFTDCVKLMATNNIGWAWWPHKKIQSIAGPLSAYLLPGYQNLLNY
                     WSGSAPRPSATVAMNALMAQANMLLIENCVYQKDVVDALIRQPNSTEIIPFAENQVPG
                     IVHATNYDLGQMLFAYNDTEYENLGGGTWNNGWNYRNDGVDIEACNDFASNGFNIGWI
                     ETGEWLKYTINVTQSGLYDINLNISAPNTGGNIILSLDGQSITGLLAVPATGGWQNWQ
                     YLMVPNVYLSAGIHVLQPRFFFGGFNFSYMDFVLTAVSVGNENDAPLSYELQQNYPNP
                     FNPSTTINYSTKENGNVEILVFDLLGREIVKLVDKEIPAGVHKVEFNGKNLSSGIYYY
                     TLKAGSFVSSKKMILLK"
     gene            1754..>2114
                     /locus_tag="VF870_09475"
     CDS             1754..>2114
                     /locus_tag="VF870_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9252461.1"
                     /translation="MKKIDKNKTVSDLAEKGIHTTIIGIFTSIILAAIKAIAGVLGNS
                     YALIADAIESTSDVFTSIIVLTGLKIARRPADETHPYGHGKAEPFAGIIVAAALFIAA
                     IIIIIQSLHEIITPHHAP"
BASE COUNT          711 a          346 c          400 g          657 t
ORIGIN      
        1 cttgtgggcg aaacaaatac agaactgttt tatcaaacat atcgaaataa ttatgtaaga
       61 aaagcagata tagattctat aaaaagttgg ggatttaatt ctatcagact tccatttcat
      121 tataatcttt ttgcaacaaa cactaacccg ccaacattta taaatcttgg ttttgaaatt
      181 atagattcat tattaggctg gtgtaaacaa aatgaaattt atttgatact tgatatgcac
      241 ggtgcaccag gcggtcaaag tgctgagccg attagcgatt acaatcccgc aaatccttcg
      301 ctttgggaaa gtgaatctaa taaaacacta acggttcaaa tttggagaaa aatagcagaa
      361 cgatataaag atgaggtttg gatcggaggg tacgacattc ttaatgaacc ggcatggaat
      421 cttcctcaaa acaatcaacc attacgtgat ctttatatta ggattactga tacaattcgt
      481 gctgttgata ataaccatct tttatttata gaaggaaatt ggttcgcaac tgattttaat
      541 ggattaacac cggcatggga tgaaaatatg gcttacagtt ttcataaata ttggaacgga
      601 aatactcagt catcaattca atatctagtt gatcttcgga acaataccaa tagaccttta
      661 tggttaggtg agactggtga aaattctaat aaatggttta ctgattgcgt aaaattaatg
      721 gcaacaaata atattggctg ggcctggtgg ccgcataaaa aaattcaatc aatagccgga
      781 ccattatctg cttatctact tcctggatat caaaacctat taaattattg gagtggatcg
      841 gcaccaagac cttctgcaac agtagctatg aatgcattaa tggctcaagc taatatgcta
      901 ctcatagaaa attgcgttta ccaaaaggat gttgtcgatg cattgataag acagcccaat
      961 agtacagaaa ttatcccctt tgcagaaaat caagttcctg gaattgtaca cgctacaaat
     1021 tacgatttgg gacaaatgct ttttgcatat aatgatactg aatatgaaaa tcttggtggc
     1081 ggaacctgga ataatggatg gaactataga aatgatggtg tggatatcga ggcctgcaat
     1141 gattttgctt ctaatggttt taatattggt tggattgaaa ctggagaatg gttaaaatat
     1201 actattaatg ttactcaaag tggtctatac gacatcaatc taaatatatc tgctccaaat
     1261 actggaggca acattatctt gtccttagac ggacaaagta ttacgggtct tcttgcagta
     1321 cctgcaactg gaggatggca aaactggcaa tacttaatgg ttccgaatgt ttatctcagt
     1381 gcaggaattc atgtattaca accaagattt ttcttcggtg gatttaattt tagttatatg
     1441 gattttgtgc ttactgctgt aagtgttgga aatgaaaatg atgcgcctct ttcttatgaa
     1501 ttacaacaaa actatccgaa cccatttaat ccttcgacaa caataaatta ttctacaaaa
     1561 gaaaatggaa acgtagagat tctcgttttt gatttactcg gaagagagat tgtaaaactt
     1621 gttgataagg aaatacctgc aggtgtacat aaagttgaat ttaatggcaa aaatttatcc
     1681 agcggaattt attattatac attgaaagcg ggaagttttg tatcttcaaa aaagatgatt
     1741 cttttaaaat agcttgaaga agatagataa aaataaaact gtttccgatc tggctgaaaa
     1801 ggggatacat acaaccatta ttgggatttt tacaagcata atacttgctg caataaaagc
     1861 aatcgccggt gttctgggaa actcctacgc tttaattgct gatgctattg aatccacatc
     1921 tgatgttttt acttcaatta tcgttttaac tggtttaaaa attgcacgcc gacctgcgga
     1981 tgaaacacat ccatacggtc atggtaaagc tgaaccgttt gccggaatta tagttgccgc
     2041 cgctttattt attgctgcga ttataataat aatacaaagc ttgcatgaaa taattacacc
     2101 gcatcatgca cctg
//
LOCUS       DASWBY010000525         3738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_475168, whole genome shotgun sequence.
ACCESSION   DASWBY010000525 DASWBY010000000
VERSION     DASWBY010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475168"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1455
                     /locus_tag="VF870_09480"
     CDS             166..1455
                     /locus_tag="VF870_09480"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX9252462.1"
                     /translation="MISTISKTTRKTKMTEGNIYNNEDLFQIKDFMLVQRVIKRINSI
                     LDLECLLEQIIEDASSTLGFTKCGVLLYEESTDQLRLAAVTGWEDKIHKVGDRFNLGE
                     GIVWRAFIAKKVMYYPDINEYPEEIHCDFTSQSHIDIPLISKGNVIGILNAQHKNKDG
                     FSRHNIRILKTLGAHLSVAIRNAKLFEKEKLEKKLIIRELKEAQQIQTRFFPKVAPEI
                     SNFKLSGMCVPCFEVGGDWYDYIQLPSGKIGVVLADVSGKGLSAAFLMASARTVIRML
                     GMEEESPAELLKKVNRILVSDLPGSRFITLIYAVIDPQTRKINIASAGHTVPVLKSIT
                     GASFLHVNSGLPLGIKEFDYAENTVTMKKGERLFLYSDGVTEAMNYKNELFDDHRLIS
                     SLDKREADINTIYKDVKNFIADNNQSDDLTIVMIESV"
     gene            1543..2061
                     /locus_tag="VF870_09485"
     CDS             1543..2061
                     /locus_tag="VF870_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252463.1"
                     /translation="MPLRTTANNRQRKFFNDLFSIDELVLTIKNGETCSMNAHGSFEL
                     FDKHWDGIEYQPYETMIALKENKPAKICSEPSVHFHINWLEVKYVSIRLRRLELINTD
                     YEIVFCNERDTNARVFWFYARYDSALRCLIANWNQSWINLCEGSISNLENIELKSIEY
                     SQKFEVREMQEY"
     gene            complement(2199..2444)
                     /locus_tag="VF870_09490"
     CDS             complement(2199..2444)
                     /locus_tag="VF870_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252464.1"
                     /translation="MNEIKKEELKFENEIKFLDLIYTDMIEALHQKPDENDIEAMRLY
                     MDNIWGVFNRTIFRVTEIKNNLQKDQKLRHETWNPPA"
     gene            complement(2459..>3738)
                     /locus_tag="VF870_09495"
     CDS             complement(2459..>3738)
                     /locus_tag="VF870_09495"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEX9252465.1"
                     /translation="GNKGAVWVAVKIPDGYISGHANQSRITTFPLNDPDNCLYAPDVI
                     SFARDKGYFSGKDSEFSFSDAYAPLDFGAIRFCDARVWSLFRRCNSTMDKYISYIKGE
                     SLERMPLYIKPDKKISVHDVMGLMRDHYEGTKLDMTKGVAAGPYQMPYRWRPLTWEYN
                     GEEYFHERPISTPQTGFSFVSQARANLPREVGGLFWFGVDDTYFTVYTPMYSSMTKVP
                     YNFSHGVGSLSDFTWDSAFWVFNFVANFSYPRYSVVIDDVKMKQNELEGRYFAQQENI
                     ESTALSLLKNSKGEAIEYLTNYSITSAENTYKTWKQFGEHLLLKYMDGLSKTEFFKPK
                     NIGYPEEFKKLIVEESGDALKMKTLTTDQTAVYNENIKIADKLLNEKKYSEAKGYYEK
                     ALAIKPTETYPKEKIDKLNSILGTIEELHKNTF"
BASE COUNT         1298 a          574 c          649 g         1217 t
ORIGIN      
        1 aatagttaag cgagaaggat ttaaggctct tctaaaaaaa agaggccgga aggttttagc
       61 atttgtaatt gggtactata caattagaga tacactggtt tacatagtta ttccgctatt
      121 aatagcagaa ggaatttttt aataattaaa ttagattttt ataagatgat atcgacaatt
      181 tcaaaaacga caagaaaaac aaaaatgact gaaggaaata tctacaacaa cgaagatctt
      241 tttcagatta aagactttat gctcgttcaa agagtaatta aaagaattaa ttcaatcctg
      301 gatctggaat gtttattgga acaaataata gaagatgcat caagcacttt aggttttaca
      361 aaatgcggag tgcttcttta tgaggaaagc acagaccaat tacgactggc tgcagttact
      421 ggctgggaag ataaaattca taaagtagga gatagattta atttaggaga aggaattgtt
      481 tggagagctt ttatagcaaa aaaagtaatg tattatccgg atataaatga gtatccggaa
      541 gaaattcatt gtgattttac gagccaatcc cacatagata ttcctttaat ctcaaaaggt
      601 aatgtcatcg gaatattaaa cgcacaacat aagaataagg atggattctc aaggcataac
      661 ataaggatat taaagactct aggagcgcat ctttcggtag ctattaggaa tgcaaaacta
      721 tttgaaaaag aaaaactaga aaaaaaatta ataataaggg aacttaaaga ggcacagcaa
      781 atacaaacta gatttttccc gaaagttgcg ccggaaattt ctaactttaa actatcagga
      841 atgtgtgtac catgttttga agtcggaggt gactggtatg attatataca acttccttca
      901 ggcaaaatag gtgttgtact cgctgatgta tctggaaaag gtttgtccgc agcttttctt
      961 atggcttcag caagaactgt aatcaggatg cttggaatgg aagaagaatc tcctgctgaa
     1021 ttacttaaga aggttaatag aattttggta tctgatttac cgggttcaag gtttatcaca
     1081 cttatttatg cagtaattga tccacaaact aggaaaatta atatagcaag tgcgggtcat
     1141 acagtacctg ttcttaaatc cataacaggc gccagttttt tgcatgttaa ttctggatta
     1201 ccacttggta ttaaagaatt tgattatgct gagaatacag ttacaatgaa aaaaggcgaa
     1261 agactttttc tgtattcaga tggtgttact gaagcaatga actacaaaaa tgaattattt
     1321 gatgatcaca gattgatatc aagtttagat aaaagggaag cagacattaa tacaatttat
     1381 aaagatgtta aaaattttat agccgacaat aaccaaagtg atgatttaac aatagtaatg
     1441 atagaatccg tttaagtgca gtaaaacttc taatccgatg atttaaagaa gtaaaataaa
     1501 tcatataata ttttaataaa attaaaagga attaaaaata caatgccatt aagaacaacc
     1561 gccaataatc ggcagcgaaa attttttaat gacttgttca gcatagatga attagtgctt
     1621 accattaaaa atggagagac ttgctcaatg aatgctcatg gttcatttga attatttgat
     1681 aaacactggg atggaatcga atatcagcca tacgaaacaa tgatcgcatt gaaagaaaac
     1741 aaaccagcaa aaatttgtag tgaaccatct gtgcatttcc atattaactg gctagaagtc
     1801 aaatacgttt caattcgact gagacgttta gaattaataa atacagatta tgaaatagtt
     1861 ttttgtaatg agcgtgatac taatgcaaga gtgttttggt tttatgcacg ctatgattcc
     1921 gctctgcgtt gtttaatagc aaattggaat caaagctgga taaatttgtg cgaagggtca
     1981 ataagtaatc ttgaaaacat agaattaaag agcattgaat attctcaaaa gtttgaggta
     2041 agagaaatgc aggaatacta gtatctagct atttcttgat actactaatt ctaattacat
     2101 acaaaagaat agatcatagt tttaaaatca tccaaattac taaatcaaaa taggtataag
     2161 gtgcttttaa tagtaaagtc tattttagtc tataaatttt atgccggcgg attccaggtt
     2221 tcgtgtctta gtttttgatc tttttgcaga ttgtttttta tctcagttac tctgaaaata
     2281 gttcgattaa aaacacccca gatattatcc atataaagcc tcattgcttc aatatcgttt
     2341 tcatccggtt tttgatgaag cgcttcaatc atatctgtat agattaaatc cagaaattta
     2401 atttcatttt caaattttag ttcttctttt ttaatttcgt tcataatatc tctattgatt
     2461 aaaatgtgtt tttatgtaat tcttcgatag taccaaggat tgaatttagt ttatcaatct
     2521 tctcttttgg ataagtttca gtcggtttga ttgccaaagc tttttcataa tacccttttg
     2581 cttccgaata tttcttttcg ttaagcagtt tatcagctat ttttatgttt tcattgtaaa
     2641 ccgctgtctg gtccgttgtg agtgttttca ttttcagtgc atcacccgat tcttcaacaa
     2701 tcaatttttt aaattcttca ggatatccaa tattttttgg tttaaagaat tcagtttttg
     2761 ataacccatc catatacttt aagagtaaat gctctccaaa ctgcttccac gttttataag
     2821 tgttttcggc agaagtaata gaatagttcg taagatattc aattgcttca cctttagaat
     2881 tttttagtaa tgataaagcg gttgattcaa tattttcctg ctgtgcaaaa tatcttcctt
     2941 ccagttcatt ttgtttcatt ttaacatcat caataacaac agaatatctt ggataggaaa
     3001 agtttgcaac aaaattgaaa acccaaaaag ctgaatccca tgtaaaatca gatagtgatc
     3061 cgacaccatg tgaaaagtta taaggaactt ttgtcattga tgaatacatt ggtgtataaa
     3121 cagtaaagta agtatcatca acaccgaacc aaaataagcc gccaacttcg cgaggtaaat
     3181 tagcgcgagc ttgagagaca aaagaaaatc ctgtttgtgg agttgaaatc ggacgctcgt
     3241 gaaaatattc ttcaccatta tattcccagg ttaaaggtct ccatcgataa ggcatttgat
     3301 atggaccggc ggcaacacct ttggtcatat caagcttagt gccctcataa tgatcccgca
     3361 tcaatcccat tacatcgtga acagaaatct ttttatctgg tttgatgtat aaaggcattc
     3421 tttctaaaga ttcacctttt atgtatgata tatatttatc catagtagag ttgcatcttc
     3481 taaagagtga ccaaactctt gcatcacaaa atcttattgc accaaaatcc aatggggcat
     3541 aggcatcgga aaaactgaat tcagaatctt ttcctgaaaa gtatccttta tctctagcga
     3601 atgaaattac atccggggca tacaaacaat tatcaggatc atttaatggg aaagttgtaa
     3661 tgcgtgattg attagcatga ccggaaatgt atccatcagg aatttttact gcaacccaaa
     3721 ctgctccttt gtttcctt
//
LOCUS       DASWBY010000526         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_699368, whole genome shotgun sequence.
ACCESSION   DASWBY010000526 DASWBY010000000
VERSION     DASWBY010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699368"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VF870_09500"
     CDS             complement(<1..674)
                     /locus_tag="VF870_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEX9252466.1"
                     /translation="METLITNHQSLAFFNSCGFLKEEYHKWHSQFIDREFEMLVFGHA
                     GFPVIIFPTSRARYYQAKDFGLINAAEYLIDTGKIKIYCPDSLDNLSWYNTNIYPADR
                     VKTQIAYEEVILNDVIEFAFQDTGFDKVGLSGCSFGGYHSANIAFRNPDKVRYLFTMG
                     GASNIKRFLDGYYDDNCYFNNPPDYLANLTDDKILDAIKKIGIILGTGEFDMCLDENK
                     ELSRILS"
     gene            821..>1654
                     /locus_tag="VF870_09505"
     CDS             821..>1654
                     /locus_tag="VF870_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX9252467.1"
                     /translation="MKSKTQQTPLMAQYYKVKQAHPDTVLLFRVGDFFETFEEDAKTA
                     SKVLGITLTKRSNGSAGDTPLAGFPHHAIDSYLPKLVRAGYRVAICEQMENPKFAKGI
                     VKREVVEVVTPGVTLSDKLLDHKKNNYLLAISILDQICGLSFCDISTGEFYVFEIPTS
                     QLGEQIESINPSEFLIPKRDKEFLGKLIERINPALRITKLEDWIFNFDFANELLKNHF
                     KTVTLKGFGIEKLNAGIIAAGAVLNYLNETQRVNLSHLNRISLYNPSDYMVLDYSTKR
                     NL"
BASE COUNT          567 a          287 c          260 g          540 t
ORIGIN      
        1 gataaaattc ttgataactc tttattctca tctaggcaca tatcgaattc accggttcca
       61 agaataatcc ctatcttttt aattgcatcc aaaattttat cgtcagttaa atttgcaaga
      121 taatctggtg gattgttgaa ataacaatta tcatcataat atccatctaa aaatctttta
      181 atattggaag ctccgcccat cgtaaataaa tatctaactt tatccggatt acggaatgca
      241 atgtttgcag aatgatatcc accaaaactg catcctgata aaccaacttt atcaaatcct
      301 gtgtcctgaa aagcaaactc aattacatca ttaagaatta cttcttcata agctatttga
      361 gtttttactc tgtctgcagg ataaatgttc gtattatacc agcttaaatt atctaaacta
      421 tcaggacagt aaatttttat ctttccggta tcaatcaaat attctgcggc atttatcaaa
      481 ccaaaatctt tagcctggta atatcttgct ctggaagttg gaaaaataat tacgggaaat
      541 ccagcgtgac caaaaaccaa catttcaaac tcccgatcaa taaattggga gtgccattta
      601 tgatattctt ctttcaaaaa tccacacgaa ttaaaaaagg caagagattg atgatttgta
      661 attagagttt ccatcaatct tactatttgc cggcaaatat atgaaaaaat gattttagat
      721 caatcattca aaatattctg gaaataataa tcagaagtct ctatgaaata ataatagata
      781 catatttgaa tgctaaattc attattttta ccccacaatt atgaaatcaa agacacagca
      841 aacgccccta atggcgcaat attataaggt aaagcaggca catcctgata cggtgctgtt
      901 atttagggtt ggcgactttt ttgaaacttt tgaggaagat gcaaaaacag cttcaaaagt
      961 gcttggaatt acattgacca aacgttcaaa tggttcagcc ggcgatactc cattggctgg
     1021 ctttccgcat cacgcaatcg atagttatct gccgaaactt gttcgtgctg gttatagagt
     1081 tgcaatttgc gaacaaatgg aaaatccaaa gtttgcgaaa gggattgtta aacgtgaagt
     1141 ggttgaagtt gtaactccgg gcgtaactct ttctgataaa ctgcttgatc ataaaaaaaa
     1201 taattatctt cttgcgattt caatcttaga tcagatttgc ggattatcat tttgtgatat
     1261 ttcaaccggc gagttttatg tttttgaaat tccaacttct caactcggtg agcagattga
     1321 atcaattaat ccttcagagt ttttaattcc taaacgtgat aaagaatttt taggaaaact
     1381 gattgaaaga attaatcctg ctttaagaat tacaaaactt gaagattgga ttttcaattt
     1441 tgattttgca aatgagcttc taaaaaatca ctttaaaact gtgacactta aaggctttgg
     1501 aattgaaaaa cttaatgcag gaattattgc cgctggggca gttctaaatt atcttaatga
     1561 aactcaaaga gtcaatcttt cgcatctaaa cagaatatca ctttataatc catctgatta
     1621 tatggttctg gattactcaa caaagagaaa tctt
//
LOCUS       DASWBY010000527         4497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_207021, whole genome shotgun sequence.
ACCESSION   DASWBY010000527 DASWBY010000000
VERSION     DASWBY010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207021"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VF870_09510"
     CDS             complement(<1..1253)
                     /locus_tag="VF870_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9252468.1"
                     /translation="MKKLFLVILVLSGILLAQNNNPEITSGEIKDHIAYLASDELEGR
                     MTGTHELYKAAEFLKNEFESYGLKPLFNGSYFQEFPFMEKLELGDNELTIIANGKEKK
                     LELSKDFTPLGFTDNQKADGNIVFAGYGITAKDLNYDDYSGLDVKDKIVIVFRNHPDM
                     KTPHSKFDQYSSLRYKTTTARDNGAKGIIFVNTKDNKDDALVEFKYDNAAKIKDISVV
                     SAKRESLNFIPLVELQNQIDSTLAPKSYLLESNIKASISTEVIEVQGKSVNVGAYLDA
                     ANDKFKDEYLVLGAHFDHLGWGGQNSLYMGEPAIHNGADDNASGTSGLLELAEKFASI
                     KNQLDRKIVFIAFSGEELGLLGSSYVVNNFPLPIENDITMINMDMIGRLNDKNDLIVY
                     GTGTSSKWKNILDDKNEYDLNLTFN"
     gene            complement(1308..2345)
                     /locus_tag="VF870_09515"
     CDS             complement(1308..2345)
                     /locus_tag="VF870_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252469.1"
                     /translation="MKSVIFYCSILILSYSVSFFAQEKNYSFEGEKHFKNMKMLTTDG
                     ENAEAYFSFDGKKLVFQSTIDSMECDQIFTMNIDGSNRQLVSTGKGRTTCSYFYPDET
                     KIIYASTHQFDEKCPPPPDRSIGYVWKLYESFDIFLKDGDTLKQLTDTPGYDAEATVS
                     PKGDKIVFTSLRNGDPEIYTMNLDGSEQTRLTFETGYDGGPFFSPDGSKIVFRASRPK
                     TEEQMADYKDLVENGYVRPTALEIFVMDADGKNMKQITNLGKASFAPFFHPSGKKIIF
                     ASNYEGDNPRDFNLFMINIDGTGLERITFNPSFDGFPIFTPDGKYIVFGSNRFNNKET
                     DTNIFIAEWID"
     gene            complement(2381..3097)
                     /locus_tag="VF870_09520"
     CDS             complement(2381..3097)
                     /locus_tag="VF870_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9252470.1"
                     /translation="MKKNKYPISWEKENFSIKIFCSIPNIATGILVKADDAHFIVDPG
                     DGILRDLNKELNVKNILNISDVLVTHGHHDHVGGVWSLLTYLRVMNKQTPLVIHYPKG
                     CLEIVHIYNAFKKVYGSSITYDLILKEISGDKSFTSKKVSIKPFPVIHKEYLSDNKTT
                     RQVPALGFKFTFNKIKICYGGDTAYCDDLVKHAKGSDLAILEAGHDDDTPDEMHMTLS
                     EARSIGKSAKEYFLVHVPEK"
     gene            complement(3125..3574)
                     /locus_tag="VF870_09525"
     CDS             complement(3125..3574)
                     /locus_tag="VF870_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX9252471.1"
                     /translation="MRNEKAAEVFRTFLKRGKNRITPERFEVLDAALEHNGHFGADDL
                     FIQLKNLKSKVSRATVYNTLELLAQCDLLSKRNFGDNLTRFESNFKRQTHDHLICVKC
                     GKIVEFSDGRISEIPREISEKFGFEMESYSFNIFARCKNEKECKSRK"
     gene            complement(3720..>4497)
                     /locus_tag="VF870_09530"
     CDS             complement(3720..>4497)
                     /locus_tag="VF870_09530"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX9252472.1"
                     /translation="TPADKAWLAGIALAIIYISSILGSLVIAAFINKFNKKIINAVDS
                     SSFILELPAYRAPKIKNVIRNSFESAKQYIYRAGPIILVLSIAIWFLTYFPNHSPQIN
                     TTGLSQQEAVQMVKAERLSTSFAADLGKVIQPVMSPLGMDWRIGVSLIAAFAAREVFV
                     SSLAMIFKVTDDDNLQSSILLAMKNAVNEETGKPLFTVPTIIGLIVFFIFALQCISTV
                     AVSKKETGGWRIPILQIIIFTTSAYVLSLITVNLLKLAGF"
BASE COUNT         1400 a          836 c          651 g         1610 t
ORIGIN      
        1 tcgttaaaag ttaaatttaa atcatattca tttttatcat ccagaatatt cttccatttt
       61 gacgatgttc ctgttccata aacaatcaaa tcatttttat catttaatct tcctatcata
      121 tccatattta tcatagttat atcattctca atagggagcg ggaaattatt tacaacataa
      181 cttgatccaa gtagtccaag ttcttcacca gaaaaagcga tgaaaactat ttttctgtct
      241 aactgatttt ttatcgatgc gaacttttca gccaattcca ataaaccgga tgtacctgaa
      301 gcattatcgt cagctccatt gtggatagca ggttcaccca tatacaatga attttgtccg
      361 ccccagccga ggtgatcaaa gtgtgcacca agaaccaggt attcatcttt aaatttatca
      421 ttcgcagcat caaggtaagc gccaacattt acacttttac cttgaacttc aatcacttct
      481 gtcgatatcg atgctttaat atttgattct aataaatatg attttggtgc tagagttgaa
      541 tcaatttgat tctgtaattc taccagtgga ataaaattta agctttctct ttttgcacta
      601 actactgaaa tatctttgat ctttgccgca ttatcatatt taaattcaac aagtgcatca
      661 tccttattat cttttgtgtt aacaaagatt attcctttag caccattgtc tctagcagta
      721 gtagttttat aacgaagact tgaatactga tcaaactttg agtgcggagt tttcatatca
      781 gggtggtttc tgaaaacaat cacaatctta tctttaacat caaggccaga atagtcgtcg
      841 taattaagat cttttgctgt aattccatat ccagcaaaaa caatatttcc atctgccttc
      901 tgattatcag taaatccaag tggagtaaaa tctttcgata gctcgagttt tttttcttta
      961 ccatttgcga taattgttag ttcattatca cccagttcaa gtttttccat aaaaggaaat
     1021 tcctgaaagt aacttccgtt aaaaagcggt tttaatccat aactctcaaa ttcattctta
     1081 agaaactctg ccgctttata taactcatgt gttccggtca ttctaccttc aagttcatca
     1141 gaggcaaggt aagcaatgtg gtcttttatt tcacctgatg ttatttcagg attattattc
     1201 tgtgccaata atattccaga taataccaaa ataactaaaa acaatttttt catttatctg
     1261 ctttctactt ttttaattac taattacttc ctactattta acaacaacta atcaatccat
     1321 tcagcaataa agatatttgt atcagtttct ttattgttaa atctgtttga tccaaataca
     1381 atatatttgc catcgggagt aaaaattgga aatccatcaa acgaaggatt aaatgtaatg
     1441 cgttctaaac ctgttccgtc aatatttatc ataaacaaat taaaatctct tggattatca
     1501 ccctcataat tagatgcaaa aataattttc tttccgcttg gatgaaagaa gggagcaaaa
     1561 cttgctttac ccaaatttgt aatttgcttc atattttttc catctgcatc cattacaaaa
     1621 atttctaatg cagttggacg aacataacca ttttcgacaa gatctttgta atcagccatc
     1681 tgttcctcag tctttggtct gcttgcgcgg aatacaatct tgcttccatc tggtgaaaag
     1741 aatggtccgc catcataacc tgtctcaaaa gttaatcttg tttgttcact gccgtctaaa
     1801 ttcatcgtgt aaatttcagg atcaccattt cttaaagatg taaaaacaat tttatctcct
     1861 ttaggtgata ctgttgcttc tgcatcataa ccgggagtat cagttaattg ctttaaggtg
     1921 tcaccatcct tcaaaaaaat atcaaacgat tcgtatagtt tccatacata acctatagat
     1981 cgatctggtg ggggaggaca tttttcatca aattggtgtg tagaggcata aataattttt
     2041 gtttcatccg ggtaaaaata agaacaagtg gttctgcctt ttccggtaga tactagttga
     2101 cgattgcttc catcgatatt cattgtaaat atctgatcgc attccataga atcaatcgtt
     2161 gactgaaaaa ctaatttctt cccatcaaaa gaaaaatatg cttcggcatt ttccccatca
     2221 gttgtaagca ttttcatatt tttgaaatgc ttttcacctt caaatgaata atttttttcc
     2281 tgagcaaaaa acgatacact ataactaaga attaatatcg aacaataaaa aataactgat
     2341 ttcatatgcc tcaactcaat taattaataa ttctttactt ttacttttcc ggtacatgaa
     2401 caagaaaata ttctttagca gatttaccga ttgatcttgc ctcgcttaaa gtcatatgca
     2461 tttcatcggg agtatcatca tcgtgcccgg cctctaaaat tgctaaatct gaacccttag
     2521 catgttttac aagatcatca caataagcag tatcgccgcc ataacagatt tttattttat
     2581 taaatgtaaa tttaaatcct aatgcaggta cctgtctggt tgttttatta tcggataaat
     2641 attctttatg aataacagga aaaggtttta ttgaaacttt tttactggta aacgatttat
     2701 cacctgatat ttcttttaaa attaaatcat acgtaatgct gctgccgtaa acttttttaa
     2761 atgcattgta tatatgaaca atctctagac agcctttggg ataatgaatt acaagaggtg
     2821 tctgtttgtt catcactctt aagtaagtta ataaagacca tacgccgcca acatgatcat
     2881 gatgaccatg cgttacaaga acgtcggaaa tattaagtat gttttttaca ttcaattcct
     2941 tgttcaggtc tcgtaatatt ccatcaccgg gatcgacaat aaaatgtgca tcatctgctt
     3001 ttactaaaat tcctgttgca atattgggta tggagcagaa tattttgata gaaaagtttt
     3061 ctttttccca gcttattgga tacttatttt ttttcattta ttaagagttg tttattcata
     3121 gaaattattt tctgctcttg cattctttct catttttgca ccttgcaaaa atattgaatg
     3181 aataactttc catttcaaaa ccaaatttct cactaatttc cctgggtatt tcactaattc
     3241 tgccatcaga aaattctaca attttaccgc acttaacaca gattaaatga tcgtgggttt
     3301 gccgtttgaa gttgctctca aacctggtaa ggttatctcc aaaatttctt ttacttaaaa
     3361 gatcgcattg tgcaagtaat tccagggtat tataaacggt agcgcgagat actttagatt
     3421 ttaaattctt taactgaata aataaatcgt ctgcaccaaa atgcccatta tgctcaagag
     3481 cagcatccag aacttcaaat ctttctggag ttattctgtt cttgcctcgt ttaagaaagg
     3541 ttctgaatac ttccgctgct ttttcgtttc tcactttttg ctcacttgtt actacttgaa
     3601 aataatttca tactatacta atatcaagga ttatatagtt taatttttag acgggctttt
     3661 tccagtttaa taaaattatt tactaattat tatataaaac atatttatgc tgctgcattc
     3721 taaaaacctg caagttttaa tagatttact gttatcaacg ataaaacata agctgaagtc
     3781 gtaaaaataa ttatctgtaa aattgggatt ctccatccac cggtttcttt ttttgaaacc
     3841 gcaactgtgg atatacactg caacgcaaat atgaagaata ctattaaccc gattattgtg
     3901 ggaacagtaa ataaaggttt acctgtttct tcatttaccg cattcttcat tgccaataga
     3961 atagatgact gcaaattatc gtcatctgtt actttgaaga tcattgctaa tgaacttaca
     4021 aatacttccc gagctgcaaa tgcggcgata agcgatacac caatccgcca atccattccc
     4081 aaaggactca taaccggttg tatgactttt ccaagatctg ctgcaaatga tgttgaaagt
     4141 ctttctgcct taaccatttg aacagcttct tgttgtgata aaccagttgt atttatttgc
     4201 gggctatgat tcggaaagta tgttaagaac catatcgcaa ttgataatac taatattatt
     4261 ggtcccgctc tataaatata ttgttttgca ctctcaaagg aatttctgat tacgttttta
     4321 atttttggtg ctctgtacgc aggcaactca agtataaaag acgaactatc aaccgcatta
     4381 attatttttt tgttaaactt gtttataaat gcagctatta ctaatgagcc taatatgctt
     4441 gaaatgtaga ttatggctaa ggcgattcca gccagccaag ctttatctgc aggagtt
//
LOCUS       DASWBY010000528         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_658805, whole genome shotgun sequence.
ACCESSION   DASWBY010000528 DASWBY010000000
VERSION     DASWBY010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658805"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VF870_09535"
     CDS             complement(<1..452)
                     /locus_tag="VF870_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX9252473.1"
                     /translation="MKVDFMYKINLKTGGSKIIYNPLSVSLKYLIDDKKLAKSLPNLE
                     KIFNIKFSYLQRKTFLSGEGGKIRVTKPSGKPDALILSKVKLDDKFDADYFRNHLAEF
                     ISTLKNEATKNLHIFIPAYSAVKKYFESEEYYYQTFAEGVLLGNYSFD"
     gene            complement(449..1435)
                     /locus_tag="VF870_09540"
     CDS             complement(449..1435)
                     /locus_tag="VF870_09540"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX9252474.1"
                     /translation="MIDFEKLKNIILDNSSFLLTTHVNPDADAIGSEMAFYYLLNKLD
                     KKAYIINHSALPYNMEFFNKENVIQRFDEENHKNIFNEVDVLVALDFNRADRTVRMEK
                     GFRKSNKLKICIDHHQDPEEFVDHQFIDTDYCATGQIIYDFINQTKIVDLDFEIAEPV
                     YAAIMTDTGSFRFDRTTPQVHLAIAELLKLGVNPEQVYDKLYDQSKFSKVKLLGRALN
                     SITLLADNKIGYMTITQKDFEELGAIESDTENFVNHNLSIENVVLGILFIELKNGFKV
                     SFRSKGNIPVNKLAKEFSGGGHTNAAGARFFTNNMQEMIPVILSKAELYLNK"
     gene            complement(1637..>2283)
                     /locus_tag="VF870_09545"
     CDS             complement(1637..>2283)
                     /locus_tag="VF870_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9252475.1"
                     /translation="RIYGLTKGQYSPTSEKGKVTKTTPFGSVDYPFNPTMLATGSEAT
                     FVARPIDRHTKHLQDMIKRVGAHKGTAFLEIYQNCNIFNDGAFSVLTEKETKDDNVLV
                     LEHGKPMVFGKDSSKGIKLDGFTPRVVDLTSGQYSVNDLWIHDEFEESYVRSAILSSF
                     TETPGFPTPIGVFRQVFKPTYDEGLVAQIEDVKKKKGVGELEKVLFGGNTWEVN"
BASE COUNT          726 a          406 c          337 g          814 t
ORIGIN      
        1 ttatcaaaag aataattgcc aagtaaaacg ccttcagcaa aagtttgata gtaatattct
       61 tcagattcaa aatatttttt tactgctgaa taagccggaa tgaatatgtg gagatttttt
      121 gttgcttcat tctttaaagt agaaataaat tctgccagat ggtttctgaa ataatctgca
      181 tcaaatttat cgtccagttt aactttggac aggattaatg catccggctt tccggatggt
      241 ttagtaacac gaatttttcc accttcacct gataaaaatg tttttctctg caaataagaa
      301 aactttatgt taaagatctt ttctaaatta ggtagactct ttgctaattt tttatcgtca
      361 ataagatact ttaggctgac tgaaagtgga ttataaataa tttttgatcc accagtttta
      421 agatttattt tgtacataaa gtctactttc atttatttag ataaagttct gcttttgaaa
      481 gaattacagg tatcatttcc tgcatattat ttgtaaaaaa cctagcacct gcagcatttg
      541 tatgcccgcc tccgctaaat tcttttgcta atttattaac cggaatattt ccttttgaac
      601 ggaaacttac tttaaaacca tttttaagtt cgatgaatag aattccaagt acaacatttt
      661 cgattgaaag attatggtta acaaaatttt cagtatcact ttctatcgca ccaagttctt
      721 caaaatcttt ctgcgtaata gtcatataac cgattttatt atctgctaaa agcgtgattg
      781 agtttaaagc cctgccaagt aattttactt tactaaattt actctgatca taaagcttat
      841 cgtaaacttg ctcaggatta acaccgagtt ttagcaattc tgcaatcgct aagtgaactt
      901 gcggcgttgt tctgtcgaac ctgaacgatc cagtatcagt cattattgcc gcgtacactg
      961 gttccgcaat ttcaaaatct aaatcaacaa ttttagtttg atttataaaa tcgtaaatga
     1021 tttgtccagt tgcacaataa tcagtatcaa taaattgatg gtcaacaaat tcttccggat
     1081 cctgatgatg atcaatacaa atttttaact tattggattt tcgaaaacct ttttccattc
     1141 ttacagttct atccgcacga ttaaaatcca gagcaacgag aacatcaact tcgttaaaaa
     1201 tatttttatg attttcttca tcaaatctct ggataacatt ttctttgtta aagaattcca
     1261 tattgtatgg caaagcgcta tgatttatga tataagcctt cttatcaagt ttatttaaca
     1321 aataataaaa agccatttcg gagccgattg catctgcatc gggattaaca tgcgttgtta
     1381 atagaaaaga tgaattatct agtattatat tttttagttt ttcaaaatcg atcataaact
     1441 ttagaatttt attttctttt catttcgatc cagatttatc gagaaacaga gatccgaacg
     1501 ttatatcaat tagatttctc agtcgggact ccttcgaaat aacagaatta attttgcaca
     1561 taaaatagaa atgggcttga agctgtttca agcccataga ataaaaaaaa ataattttag
     1621 tttaatctat ttttaatcaa ttaacttccc aggtattgcc accaaaaagc actttttcta
     1681 actcgcctac accttttttc tttttaacat cttcaatctg agcaactaaa ccttcatcat
     1741 aagtaggttt gaatacttgt ctgaaaacac caattggtgt tgggaatccg ggtgtctcgg
     1801 tgaatgatga aagaattgca gaacgaacat aactttcttc aaattcatca tgtatccata
     1861 aatcattaac cgaatattgt ccgctggtta aatccacaac tcttggagtg aatccatcaa
     1921 gtttaattcc tttgctggaa tctttaccaa aaaccattgg cttaccatgt tcaagaacta
     1981 aaacgttatc gtcttttgtc tctttttcag ttagaacaga gaaagctccg tcattaaaga
     2041 tattacagtt ctgatatatt tctaaaaatg ctgtgccttt atgcgcacca acccgcttaa
     2101 tcatatcctg caagtgcttt gtatgtctat caattggacg agccacaaag gtcgcttcag
     2161 aaccggttgc aagcattgtt ggattaaaag gatagtcaac actaccaaat ggagttgtct
     2221 ttgtaacttt tcctttttct gatgttggtg aatactgtcc tttagttaat ccatatatac
     2281 gat
//
LOCUS       DASWBY010000529         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_735937, whole genome shotgun sequence.
ACCESSION   DASWBY010000529 DASWBY010000000
VERSION     DASWBY010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735937"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VF870_09550"
     CDS             <1..472
                     /locus_tag="VF870_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX9252476.1"
                     /translation="YWWRMNGFGFTAGTIFGMGAAIVQKIFYPDATEYLSFAILLVAS
                     FSATIIVTLLTQQTNKETLIKFYKTTRPFGFWKPIKSLLPDEKKTQINSENKRDIIAI
                     CFALPWQIVLFLTGMTIILRRMDLFFYLFVLLILLSIGLYYFWFRHLSNEVRVE"
     gene            complement(533..1942)
                     /locus_tag="VF870_09555"
     CDS             complement(533..1942)
                     /locus_tag="VF870_09555"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEX9252477.1"
                     /translation="MEKRADALDALRGFAIIAMILSGSIPFSGPAALPGWMYHAQVPP
                     PDHIFNPNIPGITWVDLVFPFFLFAMGAAFPFALRKRIEQGTPYWKLSLQAIQRGFLL
                     VVFALFLQHSKPYVLSSNPETFHWIIGLIGFVILFLIYCRYPKSINPKFVLAIKITTG
                     VGAIVLFSQLIYAKGSGFLLSRSDIIILVLANVALFGSIIWLFTKDKLLIRLAILALL
                     LAFRITHNIEGSWTGVIWDLTPAPEIYKFYFLQYLFIVIPGTIAGDLFYKWMKSSDSD
                     SSSDNILKFLYLFLLTIFIIVLNLFGLYSRQLILNLIGNIVLLSTGLFLLNNSKSQLL
                     DLYKKLFSWGAFWLILGLAFESYEGGIKKDHPTLSYYFVTSGLAFLALISFSIISDYW
                     KKSKYINLLIENGQNPMIAYIAGSNFVMPILALTGLSTFLNYMLINPWLGFLKGLIIT
                     LIAALVTSFFTKKKIFWRS"
     gene            complement(1942..>2121)
                     /locus_tag="VF870_09560"
     CDS             complement(1942..>2121)
                     /locus_tag="VF870_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252478.1"
                     /translation="VQLKVYDVLGKEIATLVNEEKPAGNYEVDFNANGLSSGVYFYQL
                     RSGDFVQTNKMILLR"
BASE COUNT          765 a          360 c          341 g          655 t
ORIGIN      
        1 gtactggtgg agaatgaatg gatttggatt taccgccgga acaattttcg gaatgggtgc
       61 ggcaattgtt cagaaaatat tttaccccga tgcaacagag tacttgtcat ttgcgatctt
      121 acttgttgca tctttttcag caacgattat tgttacttta ttaactcaac aaacgaataa
      181 agaaacatta ataaaatttt acaaaacaac acgtccgttt ggtttctgga aaccaataaa
      241 atcattactg cctgatgaaa agaaaactca aatcaattca gaaaacaaaa gagatattat
      301 cgcaatctgt tttgccctgc cttggcagat cgttttattc cttacaggta tgacaataat
      361 attaaggaga atggatttgt ttttctacct gttcgttctg ttaattttac tttcaatcgg
      421 attatattat ttctggttca ggcatttatc taatgaagta agagttgaat aaaaatttta
      481 atccttaaat cgtaagaatt caatcacaat tataaattct aaattagtta gactaacttc
      541 gccagaaaat ctttttcttt gtaaagaaac ttgtaaccaa tgccgcgatt aaagtgataa
      601 tcagtccttt caaaaatcca agccacggat taataagcat ataattaaga aaagttgata
      661 atccggttaa tgcaagaatc ggcataacaa aattacttcc tgctatgtag gcaatcatcg
      721 gattctgacc attctcaatc aacaaattaa tatatttgga ttttttccag taatcagaaa
      781 taattgaaaa agatattaaa gctaagaaag ctaatccaga tgtaacaaaa taatagctta
      841 aagtcggatg atctttttta ataccgcctt cataagattc aaatgcaaga ccaagtatga
      901 gccaaaatgc gccccaggaa aataattttt tgtaaagatc aagcagttgt gattttgaat
      961 tgttcaataa aaataatccg gtagataata aaactatatt tccaatcaaa tttaaaatta
     1021 attgtctgct gtaaagtcca aacaggttta ggactattat gaatatcgtt agtaaaaaca
     1081 gataaagaaa ctttaatata ttatctgatg acgaatccga atcagatgat ttcatccatt
     1141 tgtaaaaaag gtcacctgca attgttccgg gaataacaat aaacaaatac tgaagaaaat
     1201 aaaacttata gatttccggg gcgggagtta aatcccagat aacgcctgtc cagcttcctt
     1261 caatattatg agtaattcta aatgcaagaa gcaatgcaag aatagcgagt cttattagca
     1321 gtttatcttt tgtaaacaac caaattatcg aaccaaagag agctacattt gctagtacaa
     1381 gaataattat atcgcttctg cttaacagaa aaccgctgcc ttttgcatat ataagctgag
     1441 aaaataaaac tatggctcca actccagtgg taatttttat tgcaagaaca aattttggat
     1501 taattgattt cggatatcga cagtaaataa gaaatagaat tacaaagcca attaatccaa
     1561 ttatccaatg aaaagtttca ggattactgc taagcacata tggttttgaa tgctgtagaa
     1621 aaagtgcaaa tacaacaagt agaaatcctc tttgaattgc ctgaagtgat aacttccaat
     1681 aaggtgttcc ctgttcaatt ctctttctca acgcaaatgg aaatgccgca cccattgcaa
     1741 aaagaaagaa tggaaagacc aaatcaaccc atgtaattcc tggaatattc ggattaaaga
     1801 tatgatcagg tggaggaact tgagcatgat acatccatcc cggtaatgca gctggacctg
     1861 agaaaggaat gctgcctgaa agaatcatcg cgatgattgc aaatccacgt aaagcatcta
     1921 gggcatcagc acgtttttcc attagcgtag taaaatcatt ttatttgttt gaacaaaatc
     1981 acctgatctt aattgataaa aatatacacc gcttgataat ccatttgcat tgaaatctac
     2041 ttcataattg ccagctggtt tttcttcgtt taccagtgtt gctatttctt ttcctaatac
     2101 atcatatact ttaagttgca c
//
LOCUS       DASWBY010000530         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_475559, whole genome shotgun sequence.
ACCESSION   DASWBY010000530 DASWBY010000000
VERSION     DASWBY010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475559"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1196
                     /locus_tag="VF870_09565"
     CDS             117..1196
                     /locus_tag="VF870_09565"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9252479.1"
                     /translation="MIKIKISKTTCEDFMLNFIQSKISLFIIIFLISNQILYSQNVSK
                     TGTTAASFLEIGVGGPANAMGGAFVGLANDASSLYWNVAGITNLERYEAILVHLDWIA
                     DTKLDYAGLVLPLGDFGSIGFSFTSLSMEDMKVTTVDQPDGTGEYFSSSNLAIGLSYA
                     KKLTDRFSVGITAKYIKESIWHMSAAALAIDAGTSFKTDLLGGMTIGAVISNFGTEMQ
                     MEGRDARYTIRVDEGKQGSNENIPTNIEMDSWGLPLHFQIGVSTSPVKTEDYQLIVSA
                     DAIIPNNDYQSLNVGAEFSFMNVFYIRGGYNSLFLKDSEGGLTFGAGVSSDMLLSTAE
                     VNFDYAFRDFGKLTNVHSFSLGIKF"
     gene            1237..>1575
                     /locus_tag="VF870_09570"
     CDS             1237..>1575
                     /locus_tag="VF870_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252480.1"
                     /translation="MKTIFIILITLLSNQIFCQDIFFENDNNNKKKNEIVAEIGNLKI
                     TAEEFVYSYEFGPAFPKKQKDSKLTHLNYMINEKLLALEGFETGVMEKEFPKDIYRDI
                     ESDLAAEEMFQ"
BASE COUNT          517 a          232 c          327 g          499 t
ORIGIN      
        1 caggaaaaat gggacttagt ttctaaggat gggatggata tcgcttacgg aatttatgtt
       61 tttcatgtaa gcgctcccga tataggtgag aaaatcggaa agtttgcggt gataaaatga
      121 taaaaatcaa aatatccaaa acaacttgtg aggattttat gttgaatttt attcagtcta
      181 aaatatctct atttataata atatttttaa tatctaatca aattctttat tctcaaaatg
      241 tatccaaaac cggcacaact gcagcatcat ttttagaaat aggagtaggc ggtccggcta
      301 atgctatggg cggtgcattt gttggattgg caaatgatgc atcatcattg tattggaacg
      361 ttgctggtat tactaatctt gaaagatacg aagcaatact tgttcacttg gactggattg
      421 ccgatacaaa attagactat gcaggattgg tgctgccgct aggagatttt ggtagcattg
      481 gatttagttt cacttcgctg tctatggaag atatgaaagt aactacagtc gatcagcctg
      541 atggaacggg agaatatttt agctcatcta atttggcgat tggcttatct tatgctaaaa
      601 aattgactga tagattctct gttggaatta cagcaaagta cattaaagaa tctatctggc
      661 atatgtcagc cgcagcttta gcaattgatg caggaacttc attcaaaaca gatttgcttg
      721 gcggtatgac aataggggcg gtgatatcaa actttggaac agaaatgcag atggaaggaa
      781 gagacgccag gtacactatc cgtgtggacg aaggcaaaca aggctcaaat gaaaatattc
      841 caactaatat tgaaatggat tcctggggtt tgccgcttca ctttcaaata ggagtctcaa
      901 ccagtccggt taagaccgag gattatcagc tcattgtttc tgcggatgcc attatcccaa
      961 ataatgatta ccagagcctg aatgtcggtg ctgaattctc gttcatgaat gttttttata
     1021 taagaggcgg atataattca ttattcttaa aggattctga aggcggatta acttttgggg
     1081 ctggtgtcag ttctgatatg ttgttaagta ctgcagaagt caattttgat tatgccttta
     1141 gggattttgg aaagttaaca aatgttcaca gtttttcttt aggaataaaa ttctaataac
     1201 tgtgtttcaa attagttgta taatcaaaaa aattaattga agacaatttt cataatatta
     1261 ataacgctgc tttcaaatca gattttctgc caggatattt tttttgaaaa tgataataac
     1321 aataagaaaa aaaatgaaat cgttgcagaa attggaaatc tgaaaatcac cgcagaagaa
     1381 tttgtgtaca gttatgaatt tggtccggct tttcctaaaa agcagaaaga ttcaaaactt
     1441 actcatctta attatatgat taatgaaaag ttactggcat tagaaggctt tgaaaccggt
     1501 gtgatggaaa aagaatttcc aaaagatatt tacagggata ttgaatctga tttagctgcc
     1561 gaagaaatgt tccag
//
LOCUS       DASWBY010000531         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_171363, whole genome shotgun sequence.
ACCESSION   DASWBY010000531 DASWBY010000000
VERSION     DASWBY010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171363"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VF870_09575"
     CDS             <1..230
                     /locus_tag="VF870_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252481.1"
                     /translation="RYDLNYKIENIKVLEQNDNEAKVEFTQVTTKIKGPDFKNNKTTG
                     IHTLRKDGDSWKIYSTEMTNVEFLNKDESHN"
     gene            214..726
                     /locus_tag="VF870_09580"
     CDS             214..726
                     /locus_tag="VF870_09580"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX9252482.1"
                     /translation="MNPIIEILKSDITKLNVDAIVNAANTLLLGGGGVDGAIHREAGS
                     KLLEEYRKLNGCKTGETKITNGYKLPAKFVIHTVGPVWNGGNYNEDELLSNCYYNSLK
                     LAIGHKIKTIAFPAISTGVYGFPLEPATRTAINTVKKFLKEYSKLDKVIFVCFDDKTY
                     STYKNILAEL"
     gene            complement(723..923)
                     /locus_tag="VF870_09585"
     CDS             complement(723..923)
                     /locus_tag="VF870_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252483.1"
                     /translation="MSDIKKINHCLNCDASENEIPLVNLVFAGKPAFICSSCLPVLIH
                     QPQKLVGKLDGADKISPAKDDH"
     gene            complement(961..1845)
                     /locus_tag="VF870_09590"
     CDS             complement(961..1845)
                     /locus_tag="VF870_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9252484.1"
                     /translation="MNYLGELSALLTAVLWSGTSIAFSSAAEKIGSLQLNINRMIFAS
                     LFLISTILIMGYRFDLSNSQFTNLIISGVIGLVIGDTFLFKSYQMIGARISMLLMALS
                     PAMSAILAFVFLDERIAFLGIIGIFVTISGIALVVLERNSNSKYAVTKLGIFYGILGA
                     MGQAGGLIFAKFAFEEGHITGFVATFIRVFSSVIIFLPIMLLLKKYKNPYKLFNTNKS
                     ALGVTLIGTILGPFLGITFSLIAVENTKVGIAATLMSTMPIIMLPMVKYIYKEKLSWR
                     AITGAVIAVLGVALIFLR"
     gene            2087..2455
                     /locus_tag="VF870_09595"
     CDS             2087..2455
                     /locus_tag="VF870_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9252485.1"
                     /translation="MNLNNTFGSQDFTITMADGIPVLKFNMFRATIKETQSFKSNLVQ
                     LITANHRFVIIDLSECAFIDSAIVGVMLTVVKELRNKKGDILIVALPGAISNLFIKTG
                     LDRVFKKFESLSSALASLSI"
     gene            complement(2686..3498)
                     /locus_tag="VF870_09600"
                     /pseudo
     CDS             complement(2686..3498)
                     /locus_tag="VF870_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
BASE COUNT         1211 a          605 c          575 g         1168 t
ORIGIN      
        1 agagatatga tttgaattat aaaattgaaa atatcaaagt tcttgaacag aatgataacg
       61 aagcaaaagt tgaattcaca caggtaacaa ctaaaattaa aggacctgac ttcaagaata
      121 ataaaactac cgggatacat acactaagaa aagatggaga ctcctggaaa atctattcaa
      181 ctgaaatgac caatgttgaa tttctaaata aagatgaatc ccataattga gattcttaaa
      241 tctgatataa ccaaacttaa tgttgatgct attgtaaatg ccgctaatac tttactgctt
      301 gggggaggcg gagtagatgg tgcaattcat cgggaagcag gttccaaact acttgaggag
      361 tacagaaagc tgaacggttg taagactggt gaaacaaaaa tcacaaatgg atataaactg
      421 cctgccaaat ttgtaattca tactgtgggt cctgtttgga atggtggaaa ttataacgag
      481 gatgaattac tttccaattg ttattacaac tcattaaagt tagccatcgg acacaaaata
      541 aaaaccattg cattccctgc tatttcaaca ggtgtttatg gatttccact tgaacccgca
      601 acccggacag caattaacac ggttaaaaaa tttttgaagg aatacagcaa acttgataaa
      661 gttatttttg tttgttttga tgataaaact tattcgactt ataaaaatat tttagcggaa
      721 ctttaatggt catctttagc tggtgaaatt ttatctgcac catccagttt cccaactaat
      781 ttttgaggct gatgtattaa aaccggcaag caacttgagc aaataaaagc aggctttcca
      841 gcaaaaacaa gattaaccaa agggatttcg ttttctgaag catcgcaatt caaacaatga
      901 ttaattttct taatatcact catacacatt cctttttttt attaatcctc aaataaatat
      961 ttatcttaaa aatattaaag ccacgcccaa tacagcaatt acagcaccgg ttattgcccg
     1021 ccaggataat ttttctttat aaatatattt aaccatcggt aacataatta taggcatagt
     1081 ggacataagt gttgcagcaa ttccaacttt tgtattttca acagcaatca aactaaatgt
     1141 gattcctaaa aacggaccta gaattgttcc gattaaagtt acacctaaag cagatttatt
     1201 tgtattgaat aacttgtatg gatttttgta cttctttaat aacagcatta tcggaagaaa
     1261 aattatcact gaagagaaca ctcgaataaa tgttgcaaca aatcctgtaa tatgcccttc
     1321 ttcaaaagca aactttgcaa atattaatcc cccggcctga cccattgctc caagtattcc
     1381 ataaaaaata ccaagcttag ttactgcgta tttagaattt gaatttcttt ccagaacaac
     1441 tagcgcaatt cctgaaatag ttacaaagat tccgatgatt ccaagaaatg caattctttc
     1501 atcaagaaaa acaaaagcaa gaattgcaga cattgccggc gaaagagcca ttaaaagcat
     1561 actaattctt gcgcctatca tttgataact tttaaagaga aatgtatcac ctataaccag
     1621 accgatcaca ccgcttataa taagattggt gaattgtgaa tttgataaat caaatctgta
     1681 ccccattatc aatattgtcg agattaaaaa aagcgatgcg aaaatcattc tattaatatt
     1741 tagctgaagc gaacctatct tttcagcagc ggaagagaat gcaatagatg tcccggacca
     1801 aaggacagca gttaataacg cactaagttc accaagataa ttcatttata gaaatgtttg
     1861 atagaaaagt tatgaataaa tgttgttact tcttattaaa aagatgtaag taaaagtaca
     1921 ctcaaagtat ctaataagtc aattttagaa cgatgttcat ttatattaag caccaagact
     1981 cagttaaata aaaagtgtgc atatttatca aaaaggttca gtggagttat tatattttga
     2041 taattctact tattttgaaa taaaaatatt ttacttttag atttttatga acttaaataa
     2101 cacttttggc tcgcaggatt ttacaatcac tatggcagat ggaattcctg tcttgaaatt
     2161 taatatgttt agagcaacta ttaaagagac gcagtcgttc aagtcaaatc ttgttcaatt
     2221 aataactgca aatcacaggt ttgttattat tgatttatct gaatgcgctt ttattgattc
     2281 agcaattgtt ggtgtaatgc ttacagtagt gaaggaacta agaaataaga aaggtgatat
     2341 tcttattgtt gctttacctg gggctataag taatttgttt ataaaaactg gactcgaccg
     2401 cgtatttaag aaattcgaaa gtttatcttc tgcactagcc agtttaagta tttagacttt
     2461 atctttgtca aaattattct gcagttctaa gattaaaata taacgcacat tatctgagct
     2521 atcttccctt cagaaaattt taattaaatt tttcaataaa aaacccacta actttttaga
     2581 tagtgggtta ttgattaaat cgattttctg taagctgtat tatttgatat ctatttgttt
     2641 cttttacact tttaaattcc tatagcttat cttcataata cagttttaga gattaaaata
     2701 cattttatac tcgaacggat gaggcattgt accaattgaa tgaatttctt cattcttagt
     2761 tttaacccat tgttctaata attcatctgt aaagatgttt ccccgtctta agaaatcatt
     2821 atcagcggcc agcgcatcta aggcctctgt aagatttctt ggtagaaaat gtatcttttc
     2881 attaaaggct ggatcaagaa aatttttatc cattggtccg tacccttctt taacgggatc
     2941 aattttattt acaactccat caattcccgc aagaagcatt gcaactgtac ataaatatgg
     3001 atttgcagtt gcatcaggcg ggcgatattc aaatcgcgtt tcatcgggat tggaaacata
     3061 ttttggaatt cttatagcac ttgcacgatt gccctgcccg tatgttaacg caactggtgc
     3121 ttcaaatccc gggaccaatc ttttataaga atttgtactt ggatttgtaa acgcagaaag
     3181 tgccggtgca tgctttaaca ttcctccaat aaagtatctg ccaagttcat ttacgtttcc
     3241 gtactcacct tttttataga aagcattttt tccattcttt gttagatata aatgcaaatg
     3301 cattccattg cctgcctgct ggtacatcgg ctttggcatg aatgtaatga acagacattt
     3361 ttgcttcgca aaattgaaca aaatatattt tgcggttatg atgttatcag cggttgttaa
     3421 taaatcacta aagtaggttt caatttcctg ctgtcctctt tcaccgactt cgtgatgatg
     3481 atatttaaca tttattccct gagcttttaa agaaaactac aggcgcggat aaatcctggt
     3541 ggggaccaga attagagtt
//
LOCUS       DASWBY010000532         1748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_357863, whole genome shotgun sequence.
ACCESSION   DASWBY010000532 DASWBY010000000
VERSION     DASWBY010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357863"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VF870_09605"
     CDS             <1..1082
                     /locus_tag="VF870_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252486.1"
                     /translation="FIGWGNVGHRIINTKTILSVNPEMSFWGSWSDSLAYHGSDADNR
                     KNIDPTEGPKHYIDIDNYPEFVANGNISSNFDSLVLQHGYSFVMDQGILPWAIMNIFD
                     SLQTAFQNNQFHKAMLFAADLGHYIGDMHMPLHITKNYNGQLTGQTGIHSRYESTMIG
                     TYSAQIIYSGDSLVYINNVSEFVFNALYNNYGYVDSVLRCDSQAKAFAGNTNSTAYYS
                     KLWELSKGFTINLFKEASFKLTCLIYTAWLNAGSPVSVQDENYTQSPVAFSLYQNYPN
                     PFNPSTIIKYTISNVTLSGVEGSRVQLKIYDILGNEISTLVDEEKPAGNYEVKFDALN
                     LSSGIYFYKLQTGNSTETKKMVLMR"
     gene            complement(1134..>1748)
                     /locus_tag="VF870_09610"
     CDS             complement(1134..>1748)
                     /locus_tag="VF870_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9252487.1"
                     /translation="DMVRRVGGKIQRAVENYFAKNNLSKELEGYAWEFNVVESPEVNA
                     WCMPGGKVVVYTGILPITKDETGLAVVMGHEIAHAVAQHGAERMSQGLLQELGGVALS
                     VALAKEPAETQNLFYAAYGVGTTVGVMLPFSRTHESEADRLGLIFMAMAGYDPNAAVD
                     FWTRMAAQKGGAAPPEFLSTHPSDATRIADIKKELPEALSYYKK"
BASE COUNT          544 a          343 c          310 g          551 t
ORIGIN      
        1 tgtttatcgg ctggggaaat gtcgggcata gaattataaa cacaaaaaca attctttctg
       61 taaatcctga aatgagtttc tggggaagtt ggtctgattc gcttgcctat catggatcgg
      121 atgcggataa tcgaaaaaac atcgacccaa cagaaggacc aaaacattac attgatattg
      181 ataattatcc cgagtttgtt gcaaacggaa atatctcttc taattttgat tctttagtac
      241 tgcagcatgg ttattctttt gttatggatc agggtatact gccttgggca attatgaata
      301 tattcgattc acttcaaaca gcctttcaga ataatcaatt tcacaaagca atgctgttcg
      361 ccgctgatct tggtcattac atcggtgata tgcatatgcc gcttcacata acaaaaaatt
      421 ataatggtca attaactggt cagaccggaa tacacagcag atatgaatct acaatgattg
      481 gtacttatag tgcacagatt atttattccg gagatagttt ggtttacata aacaatgtat
      541 ctgaatttgt ttttaatgcg ctttataata attacggtta tgtggattca gttttaagat
      601 gcgatagcca ggcaaaagcc tttgccggga acacaaacag cacagcatac tattcaaaat
      661 tgtgggaact tagcaaaggt tttacaataa atttgtttaa agaggcatca tttaaactga
      721 cgtgtttgat ttatacagcc tggttaaatg ccggcagtcc agtttcggta caagatgaaa
      781 attatacaca atctccagtt gcatttagct tatatcaaaa ttatcccaac ccatttaatc
      841 cttcgacaat aattaaatat acaatttcaa atgtcaccct gagcggagtt gaaggatcac
      901 gagtacagct taaaatttat gatatcttag gaaatgaaat ttcaactctt gttgatgaag
      961 aaaaaccagc aggaaattat gaggttaagt ttgatgcatt aaatctttca agcggtattt
     1021 acttttacaa attacaaact ggtaattcta ctgaaacaaa gaagatggtt ttaatgagat
     1081 aataattaat cccaaagccc tccctttgaa ggagggttgg gaaaagactt tttttatttc
     1141 ttgtaataac ttaaagcttc aggtagttct ttttttatat ctgcaatcct tgttgcatcc
     1201 gatgggtgag tacttaaaaa ttccggaggt gctgcaccac ctttttgcgc tgccattctt
     1261 gtccagaaat caactgcagc atttggatca tatccagcca tcgccataaa aattaatcct
     1321 aatctatcag cttcgctttc gtgcgttctg ctgaatggaa gcatcacacc tacggttgta
     1381 ccaacgccat aagctgcata aaaaagattc tgggtttctg cgggttcttt agccaaagcg
     1441 acagaaagtg caactccacc aagttcttgc aataaacctt gactcattct ctcggcacca
     1501 tgctgtgcaa cagcgtgagc aatttcatgt cccataacaa ctgccagacc tgtttcatct
     1561 ttcgtgattg gaagtattcc cgtgtaaaca acaactttac cgccgggcat acaccatgcg
     1621 tttacttcag gactttcaac aacattaaat tcccaggcat atccttcaag ctctttcgat
     1681 aaattatttt tagcaaaata attttcaaca gctctttgaa tttttccgcc aacacgtctt
     1741 accatatc
//
LOCUS       DASWBY010000533         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_56057, whole genome shotgun sequence.
ACCESSION   DASWBY010000533 DASWBY010000000
VERSION     DASWBY010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56057"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1521)
                     /locus_tag="VF870_09615"
     CDS             complement(<1..>1521)
                     /locus_tag="VF870_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX9252488.1"
                     /translation="NKNLEKAIRLIKTDSEIVIDGKIDEAWNYADSTVNFFQLDPYYN
                     QPTSVKTVAKVLTTEEALYCLMICYDNKKYIQANAGMQDWYTGDIASIMLDTFGDKQT
                     AYKFAVNASGVKSDSRMLDDARNRDYSWDGIWQGTSKIYDWGYVVEMKIPYKSIKYNK
                     NLTEWGLDFDRWRSYNKEDIYWCEYEQSEGQRISKFGKLIFEDFKPSASGLNLEIYPV
                     GIAKSTYLGDNKYDFDPSAGLDMFYNPSEQLTFLLTANPDFAQIEADPFSFNISRYES
                     YFSEKRPFFTEGNEVFNPAGRQSNSGFYRPLELFYSRRIGKILPGGEEVPLIFGTKAY
                     GRYKDFEYGGFVSMTGETDYMDDGENFTEPQAFFGSARIKKQILDNSSIGVLMVGKQT
                     KDDTYGVVDIDGAFRGSDWQLAYQFARSFENSAGDYAASAGFTNFSESWGNLIRLRAI
                     GNKFDVQQIGYVPWKGTLNSVALTGPMWYLNNGAIKSMFLYFGPAVNYEDVDLYTDY"
BASE COUNT          476 a          326 c          236 g          483 t
ORIGIN      
        1 gtaatcagtg tatagatcaa catcttcata atttactgcg ggaccgaaat acaaaaacat
       61 gcttttaatc gcaccattat ttaagtacca cattggtccc gttaaagcaa cagaatttaa
      121 tgttcctttc catggaacat aacctatttg ttgtacatca aacttattac caattgctct
      181 aagccggata agatttcccc atgattcgga aaaatttgta aagccagcgg atgcggcata
      241 atcaccagca ctgttttcaa atgatcttgc aaactgataa gcaagctgcc agtcggatcc
      301 gcggaatgca ccatcgatat caacaactcc atatgtatca tctttggttt gtttaccaac
      361 catcaaaacg cctatagatg aattatccaa aatttgtttt ttaattcttg ccgaaccaaa
      421 gaacgcctgc ggctcagtaa aattttcacc atcatccata taatcggttt cgcctgtcat
      481 agaaacaaaa cctccatatt caaaatcttt atatcttcca taagccttgg taccaaatat
      541 cagcggcact tcttccccgc caggcaatat cttgccaatt cttctggaat aaaaaagctc
      601 aagcggtctg taaaaacccg aattgctctg tcttccggct ggattaaaaa cttcatttcc
      661 ttcggtaaag aatggtcgtt tttcagaaaa gtaactttca tatcggctaa tattaaaaga
      721 aaaagggtcc gcttctattt gtgcaaaatc aggattggca gttaataaaa acgttagttg
      781 ttcggatgga ttatagaaca tatccaatcc cgcgcttgga tcaaaatcat atttattatc
      841 gccaaggtaa gtagatttgg caataccaac aggataaatc tcaagattta atcccgaagc
      901 agatggttta aaatcttcaa aaatcaattt gccgaatttg gaaattcttt gtccttcgct
      961 ctgttcatat tcgcaccagt aaatatcttc tttgttataa gatcgccagc ggtcaaaatc
     1021 taatccccac tctgttaaat ttttattata cttgatggat ttatatggaa tcttcatttc
     1081 aacaacataa ccccaatcat atatcttaga agtaccttgc catattccat cccagctata
     1141 atcgcggtta cgcgcatcat caagcatacg agaatcagat tttacaccgg aagcattaac
     1201 tgcaaattta taagcggttt gtttatcgcc aaacgtatct aacattattg aagcaatatc
     1261 gccggtatac caatcctgca ttcccgcgtt tgcttgaata tactttttat tgtcatagca
     1321 aatcatcaaa caataaagag cttcttcggt tgttaaaacc ttagctacgg tttttacaga
     1381 agttggttga ttgtagtaag gatctaattg aaaaaaatta accgtcgaat cggcataatt
     1441 ccatgcctca tcaatcttgc cgtctattac tatttcacta tctgttttga ttagtcgaat
     1501 cgctttctcc aggtttttat t
//
LOCUS       DASWBY010000534         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_508771, whole genome shotgun sequence.
ACCESSION   DASWBY010000534 DASWBY010000000
VERSION     DASWBY010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508771"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2088)
                     /locus_tag="VF870_09620"
     CDS             complement(<1..2088)
                     /locus_tag="VF870_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252489.1"
                     /translation="MQKSGTIVRGFTVGTTKDFSLNSGLRLQLSGRISEDIEIVAALT
                     DENTPIQPEGNTERLEELDKVFIQVKHPNVVGTFGDYQVKQRFGEFGVIDRKLQGLMG
                     EFSFKDEYGYLSIANSRGKFNSNNFNGSDGVQGPYRVSGLNNERDIVIIAGTEKVYLD
                     GIEMVRGENNDYTIEYSNASITFTPNRLITSASRISIDFEYTDRQFARSFFGAGSSTK
                     LFDDKLQLKVQYLREGDNQDAPIDFSLTEADKVILSLAGDDRNKAVKSGVKLAEPDSL
                     GVIKGIYTKVDTIFNNTEFSYFIYNPGDSLSIYTVSFSYVGEGKGDYVRQSLGYYSFV
                     GIGKGGYLPIIFLPLPQLKQMGNIVVDVNPFKNVTLSLEYAGSLWDKNRLSDMDDGDN
                     YGYARNILFKVAQTQIQIGDLDFGKAGLTYRDRFIQGKFTSLDRFNEVEFDRNYNTSS
                     QAKSEDESLREIGISLLPLKELSINSTAGFLRQGDNYSSDRFNNLLKFSDSQNYNIEY
                     NLDYVKSVNLALKSNWLRHKGNAFYTFWQLKPGIEFLAEDKQDKQAKKDSLIDGSLKY
                     FEYSPYLELMEIYGFRMLTQFSLRDDYLPIKGVMSKESRSKTYSVEMNYNGIREVNTN
                     LVLTSRYRKYEDQFKSSGSLDNESIVIRSRTKFIFWERLLNGDFYYEVSTQKSAKLQK
                     VFVLVEKGTGNYIY"
BASE COUNT          655 a          422 c          309 g          715 t
ORIGIN      
        1 gtatatgtaa ttacctgttc ccttttctac aagtacaaaa actttttgaa gttttgcaga
       61 cttttgagtt gagacctcgt aataaaaatc accatttaat aatctttccc aaaatataaa
      121 tttcgtccgc gaacgaatta caatagattc attatccagt gaaccacttg atttaaattg
      181 atcttcatat tttcgatagc gtgatgttaa aaccagattg gtatttactt cacgtattcc
      241 attgtaattc atttcaacag aataagtctt tgacctcgac tcttttgaca tcactccctt
      301 aatcggtaaa taatcatccc gcaaagaaaa ctgtgtgagc attctaaaac cataaatttc
      361 cattaattca agatatggtg aatactcaaa gtactttaaa cttccatcaa taagtgaatc
      421 tttttttgct tgtttatcct gtttatcctc tgcaagaaat tctataccgg gctttaattg
      481 ccagaatgta tagaaggcat ttcctttgtg tcttagccaa ttacttttta gcgctaagtt
      541 tactgattta acataatcaa gattatactc aatattataa ttttgggaat cagaaaattt
      601 taaaagattg ttaaacctgt ccgaagaata attatctcct tgtcttaaaa atccagcagt
      661 agaattaatg gaaagttctt ttagaggaag aagtgagata ccgatttctc gcagtgattc
      721 atcttctgat tttgcttgtg atgaagtatt atagttacgg tcaaattcca cttcattaaa
      781 tcgatccaaa gaagtaaact tgccttgaat aaatctgtca cgatatgtta gcccggcttt
      841 tccaaaatca agatcaccaa tttgtatttg agtttgtgca actttaaata aaatgtttct
      901 tgcatatcca taattatctc cgtcatccat atcggacaac cggtttttgt cccataaact
      961 tcctgcatat tcaagactaa gtgtaacatt tttgaaagga ttaacatcaa caacaatatt
     1021 acccatttgc ttcaactgcg gcagcggaag aaagattatc ggcaggtatc cgccctttcc
     1081 aattcctaca aaactataat aaccaagact ttgcctgacg tagtcacctt taccttcacc
     1141 tacataacta aagcttacag tataaattga aagagaatca ccgggattat aaatgaagta
     1201 agaaaattca gtgttattaa aaattgtatc gacttttgta taaatacctt tgataacgcc
     1261 aagtgaatca ggttcagcaa gttttactcc tgattttact gccttatttc tatcatcgcc
     1321 cgcaagtgat aaaataactt tatctgcttc agttaatgaa aaatcaattg gtgcatcctg
     1381 gttatcacct tccctcagat actgaacttt aagctgaagt ttatcatcaa acaattttgt
     1441 tgatgatcct gcaccaaaaa aactgcgggc aaattgtcta tcggtatatt caaaatctat
     1501 actgattctg cttgcagaag ttattaaacg atttggagta aaagttattg aagcattgga
     1561 gtattcaatc gtgtagtcat tattttcgcc gcgcaccatt tcaattccat ccagataaac
     1621 tttttcagtt cctgcaatta ttacgatgtc tcgctcattg ttcaatccac ttacccgata
     1681 tggaccctgg acaccatcag agccattaaa attgtttgaa ttaaattttc ctctggagtt
     1741 tgcaatcgat aaatatccat actcatcttt aaaagaaaac tctcccatta atccctgcag
     1801 ttttcgatct ataacaccaa actctccaaa tctttgtttt acctgataat cgccgaaagt
     1861 gcctacgaca ttcggatgtt ttacctgaat aaaaacttta tcaagttctt caagtcgttc
     1921 tgtatttccc tccggctgaa tgggagtatt ttcatcggtc agcgctgcaa caatttcaat
     1981 atcttctgaa attcttcctg atagctgtaa tcgtaaacca ctgtttaatg aaaagtcttt
     2041 tgttgtgccc acggtaaagc cccgcacaat tgttccgctt ttttgcatat ctgcaccgaa
     2101 g
//
LOCUS       DASWBY010000535         3768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_358026, whole genome shotgun sequence.
ACCESSION   DASWBY010000535 DASWBY010000000
VERSION     DASWBY010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358026"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VF870_09625"
     CDS             <1..284
                     /locus_tag="VF870_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252490.1"
                     /translation="TAIIQAICGEYKIEDADDNRKGFPFIVLIIIIIVVFSFIRGGGP
                     FIPGGVYRTGTRSGGWSSGSSWGGSSGWSGGGFSGGGGSFGGGGASGSW"
     gene            403..735
                     /locus_tag="VF870_09630"
     CDS             403..735
                     /locus_tag="VF870_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9252491.1"
                     /translation="MAIKITEECINCGACEPECPNTAIYEGGVDWELAGAHYGSGDAA
                     PSGATGFYSTDFFYIVPDKCTECKGFHDEPQCAAVCPVDCCVPDPDHVEDVDTLLKRK
                     EYLDGIGR"
     gene            873..1718
                     /locus_tag="VF870_09635"
     CDS             873..1718
                     /locus_tag="VF870_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9252492.1"
                     /translation="MQIGKYKLKTILSGYFYLDGGAMFGIIPKPLWEKTNPADEINRI
                     KLTTRNLLLISDDKKILIDTGMGNKWDEKSKNIYKINPDFELEQILKLNCIQPDEITD
                     VLLTHLHFDHTGGSTKYENGKLIPTFPNARYFVQKKNFEWAMNPSDRDRGSYIKENFE
                     PLVKEGVLNLIDGEINFDENISFRIINGHTFAQQMVKISDTSNTILYCADLLPFVSQI
                     RIPYVMGYDLQPLITVQEKKKYLQLATDENWQFYFGHDPDFALATVKHSEKGIVQDKV
                     YKDFE"
     gene            1719..2048
                     /locus_tag="VF870_09640"
     CDS             1719..2048
                     /locus_tag="VF870_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX9252493.1"
                     /translation="MDDGYTKICKVSELKENQGKRFLVNDVDIAVFKVNGEIFVLNNV
                     CPHQHTSVIYDGFIEDGCVVCPAHGWMFSLTTGNQPTGAKGLDSYPVKIIDDEIFATV
                     KAKKIKW"
     gene            2253..3173
                     /gene="queG"
                     /locus_tag="VF870_09645"
     CDS             2253..3173
                     /gene="queG"
                     /locus_tag="VF870_09645"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX9252494.1"
                     /translation="MPKLTNQIVIEKAKQLGFDLVGFTKTDLLEDETEKLQQWLDKGY
                     QASMNYMEKNLHKRKDVKEILPIAKSVISLALNYYTPENHSDKNDMGKVSRYAWGKDY
                     HLIIWQKLDELETMLKEIEPEFETLSYVDTGPVMDKVWAVRAGIGWIGKHTNVINPDY
                     GSWFFIATIFTNYEFDYSEIITDHCGSCTACIDACPTNAIVQEYVVDANKCISFQTIE
                     NKEEIPVELKRKFDNWIFGCDICQDVCPWNQKFSQHTWIQEFYPHNKELNYIEIMKLN
                     NQSFKERFAESPIKRTKLKGLQRNAKFLFE"
     gene            3265..>3768
                     /locus_tag="VF870_09650"
     CDS             3265..>3768
                     /locus_tag="VF870_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9252495.1"
                     /translation="MSEQANQKNHFKVLIIGSGPAGLTAAIYTGRANLSPVIFEGMQP
                     GGQLTITTEVENYPGFEHGIDGPALMDVMRKQAQRFGAQSFYKEVTEVDFSSRPFKVK
                     SYDENYTTDSIIISTGASAKLLGLESEKKYMGYGVSACATCDGFFFKGLKIIVVGGGD
                     TAMEEANF"
BASE COUNT         1287 a          567 c          747 g         1167 t
ORIGIN      
        1 ttaccgcaat tattcaagcc atttgtggag aatacaaaat tgaggatgct gatgataatc
       61 gcaagggctt cccatttatc gtactaatta taatcattat tgttgttttt tcttttatta
      121 gaggtggcgg cccatttatt ccaggtggtg tttatagaac cggaacccga tcaggcggat
      181 ggtctagcgg cagcagctgg ggaggaagct cgggctggag tggtggtggt tttagcggcg
      241 gcggtggatc ctttggtggt ggcggggcca gtggaagctg gtaaatggtg ctaaaaacaa
      301 aacattttaa aatgtacctt gaagaatagc ctgacttttc ataaatagca tacaagttta
      361 gaaattattt actcattctg ttaatattaa ggagaataaa ttatggcaat taaaataact
      421 gaagagtgca tcaattgcgg agcttgtgag cccgaatgcc cgaatactgc gatttatgaa
      481 ggcggagttg attgggaatt agccggtgct cattatggaa gcggtgatgc agctccttct
      541 ggtgcaactg gattttactc aacagatttc ttttacattg taccggataa atgtacagag
      601 tgtaaaggat ttcacgatga accgcaatgt gctgctgttt gtcctgttga ttgctgcgtt
      661 ccagatccgg atcatgttga agatgttgat actttattaa aacgaaaaga atatctggac
      721 ggaatcggaa gataattttt tatttaaggg tggtaaaacc acccttaatg ttttatggtg
      781 ataataaaat ctcatccgga tttcacattt gcatttctaa ctctcttcat tcttatttat
      841 tttctaacat caaaatcatt taataattat ttatgcaaat cggtaaatac aaactaaaaa
      901 caattctttc gggatatttt tatttagatg gcggagcaat gtttggaata atacctaaac
      961 cattgtggga aaaaactaat ccagccgatg aaattaaccg cattaaactt accacaagaa
     1021 atcttttact tattagtgat gataaaaaga ttttaataga tacgggaatg ggaaataaat
     1081 gggatgaaaa atcaaaaaat atttacaaga taaatcctga ttttgagctt gaacaaatac
     1141 ttaagcttaa ttgtattcaa cccgatgaga ttaccgatgt attgttaacg catcttcatt
     1201 ttgatcacac aggtggttca acaaaatatg aaaacggtaa acttattccc acttttccta
     1261 atgcacgata cttcgtgcag aagaaaaatt ttgaatgggc aatgaatcca tctgatagag
     1321 atagaggaag ctacattaaa gaaaattttg aaccgctcgt taaagaaggt gttctaaatc
     1381 taattgatgg cgaaattaat tttgatgaaa atatttcttt cagaataatt aatggtcata
     1441 cgtttgcaca gcaaatggta aaaatttctg atacatcaaa tacaattttg tactgtgccg
     1501 atttattgcc ttttgtctca caaatcagaa ttccttatgt gatgggatat gatcttcaac
     1561 cattaataac ggtgcaggaa aagaaaaagt acttgcaact tgccactgat gaaaactggc
     1621 agttttattt tggacacgat cccgattttg ctttagctac tgttaagcat tcagaaaagg
     1681 gaattgttca agataaggtt tataaggatt tcgaataaat ggatgacggt tatacgaaaa
     1741 tttgtaaagt ctcagagtta aaggaaaatc aaggaaaaag atttttagtt aatgatgtcg
     1801 atattgcagt attcaaagta aacggggaaa tatttgttct aaataatgtg tgcccacatc
     1861 agcatacttc agttatttat gatggtttta ttgaagatgg ttgtgtcgtt tgtcctgctc
     1921 acggatggat gtttagctta acaaccggaa accagcctac cggggcaaaa ggactggatt
     1981 cttatcctgt aaaaattatt gatgatgaaa tatttgcaac tgtaaaagca aaaaaaataa
     2041 agtggtgaga aagttataat cccattgctt aatgaattat aattaccata actgtttaca
     2101 attgttgcaa aacataaaga ggtcatttcg aaggagtctt cgactgagaa atctgttttt
     2161 ataaaaagtt cagatttctc cctgtggtcg aaatgacaat taggttacca atgcgctaat
     2221 gaaaagtgtt tttagaattt taataaaagt gtatgcccaa actaacaaac caaatagtta
     2281 ttgaaaaagc aaagcagctt ggatttgatc ttgttggttt tacaaaaaca gatttgcttg
     2341 aagatgaaac tgaaaaactt cagcaatggt tagacaaagg ctatcaagca tcaatgaact
     2401 atatggagaa aaatcttcat aagcgaaaag acgtaaaaga aattcttcca attgcaaaaa
     2461 gtgttatctc attagctctt aattattaca ctccggaaaa tcattcagat aaaaatgata
     2521 tgggaaaggt ttcacgttat gcgtggggaa aagattatca tttaataatc tggcagaaat
     2581 tggatgaact tgaaacgatg ttgaaagaaa tcgaacctga atttgaaact ttatcctatg
     2641 ttgatacggg ccctgtaatg gacaaagtat gggctgttcg cgctggaatt ggctggatag
     2701 ggaagcatac aaatgttatc aatcctgatt acggaagctg gtttttcatc gcaacaatat
     2761 ttacaaatta tgaatttgat tattcagaaa ttataaccga tcactgtgga tcttgtactg
     2821 cttgtattga tgcctgtcca acaaatgcga ttgttcaaga atatgttgtt gatgccaata
     2881 agtgtatttc atttcaaact attgaaaaca aagaagaaat accggttgag ctaaaaagga
     2941 agtttgataa ctggattttt ggttgtgata tttgtcagga tgtttgtcca tggaatcaaa
     3001 agttttctca acatacttgg atacaagaat tctatccgca caataaagaa ttaaattata
     3061 tagaaattat gaagcttaat aaccaatcat tcaaagagcg atttgcagaa agccccatca
     3121 aaagaacaaa gcttaaagga ttacagcgta atgctaaatt tttgtttgaa tgatcagtca
     3181 accttcttga atcttcatca acttatctga atcttacaag tacaaaaaat ataattttag
     3241 ctcataatca gttttggaga aaaaatgtct gaacaagcaa atcaaaagaa tcattttaaa
     3301 gttttaatta ttggttccgg tccagctgga ttaacggccg caatttatac tggacgtgct
     3361 aatcttagtc cagtaatatt tgaaggaatg cagccgggcg gacaacttac aataacaact
     3421 gaagttgaaa attatccagg atttgaacat ggaattgatg gccctgcttt gatggacgtt
     3481 atgcgtaaac aagcgcaaag atttggagcg caatcattct acaaagaagt aacagaagtt
     3541 gatttctcaa gccgtccttt caaagtaaaa tcatacgatg aaaattacac tactgattca
     3601 atcattattt caacgggagc ttctgcaaaa ttgcttggat tagaaagtga aaaaaaatat
     3661 atgggctatg gtgtttcagc ttgtgcaacc tgtgatggct ttttctttaa aggattaaaa
     3721 ataattgttg ttggcggcgg agatacagcc atggaagaag caaacttc
//
LOCUS       DASWBY010000536         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_699907, whole genome shotgun sequence.
ACCESSION   DASWBY010000536 DASWBY010000000
VERSION     DASWBY010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699907"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VF870_09655"
     CDS             <1..722
                     /locus_tag="VF870_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9252496.1"
                     /translation="KQTNQKYFSDSVLEDEIENDNERFESLGSQDKDYDSTLYGRLFH
                     KAMEKSFYPNQIEDFLKDEFGDELKEKRISEKLLQRLQNDLIKFSRSKMFSFLTSAET
                     YKNEFEIYVADRDYFLHGIIDKIIFKEKKILIYDYKTDDIERKEIKKHAEYYLMQLKF
                     YLYIASKVFNEFDIFEGSLVFVNHPDDPVTLNYDKSKIKELELEITNIIKSIRMKNSE
                     KNIKHCKVCSYSGLTNKCIIN"
     gene            746..1204
                     /locus_tag="VF870_09660"
     CDS             746..1204
                     /locus_tag="VF870_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252497.1"
                     /translation="MKKQITLIRDIQEIEKQLIQAESGVLCIHLANEKLMQFACNFIY
                     LDKNIYTYLDSSDENYEHVKYGSAGSFSVSSSEKLTGKLKEFSYLLSCITINGEIKEV
                     DDSKLKDQIAELYRLKYSPGIKADKYEVMENLKPVILDTNEIKALIEEGN"
     gene            1249..2874
                     /locus_tag="VF870_09665"
     CDS             1249..2874
                     /locus_tag="VF870_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX9252498.1"
                     /translation="MLNYVWISLVFLGLASALFIDISDITQNTYNNDIPLTVTIKTSA
                     IKSGGGFDCEVNIPKENYNKFFKSNESENIVVHGVGSFSEDKKAIQLYLKTSENAPKK
                     LQVIAKASGKDDDILARANIISNQDSITYLSKVYFEKISFLSMKRVTNDSINIAGTAV
                     QIALGLIGIMAMWLGVMKVAEQAGLIKIIANWLKPITKKLFPEVPPDHPAVGAMVMNI
                     SANMLGLGNAATPFGLKAMEELDTLNPNKGTATNSMVTFLAINTAGMTIIPATAIAVR
                     AAAGSADPTIIIATSLFGSTCATITGLTVAKLFERFPIEKNGYGSWFKSNLKFLISVL
                     SLIAILVLTFVTGFSSVIGSAFNFLPPTFFKDMIQIVSTLAIPFFIFIFIGYGVIKKV
                     RVYEQFVEGAKEGFNIAIRIIPYLVAMLVAIAIFRAGGAMDNWLIPVLRVVTDPIGMP
                     AEALPMALMRPLSGSGSLGVMADIIAVHGPDSFIGILVSTFFGSSETTFYVLAVYFGA
                     VNIKNTRHALAAGLAADIAGALGALFIVKLLFG"
BASE COUNT          991 a          439 c          534 g          927 t
ORIGIN      
        1 acaagcagac aaatcaaaaa tatttttctg attctgttct tgaagatgaa attgaaaatg
       61 ataatgagcg atttgagtcc ctcggcagcc aggataagga ttatgattct acgttatacg
      121 gtaggttatt tcataaagcg atggaaaaat ctttttatcc taatcagata gaggattttc
      181 taaaagacga atttggtgat gaattaaaag aaaaaagaat atctgaaaaa ctgctccaaa
      241 gactgcaaaa tgatttaata aaatttagca gatcaaaaat gttttccttc cttacttcgg
      301 cagagaccta taaaaatgaa tttgaaatat atgttgcaga ccgtgattat tttttgcacg
      361 gaattatcga taaaatcata tttaaggaaa agaaaatttt gatttacgat tataaaactg
      421 atgatatcga aaggaaagaa attaaaaaac atgccgaata ttatttaatg caattgaaat
      481 tttatttata tattgcaagc aaggtattta atgaatttga catttttgag ggtagtttgg
      541 tttttgtaaa tcatcccgat gatcctgtta cgcttaacta cgacaaaagt aaaattaaag
      601 aactggaatt agaaataacg aatattatta aatcaatcag aatgaagaat tcagagaaaa
      661 acataaaaca ttgtaaagtt tgttcttatt ccggattaac aaataaatgt attataaact
      721 aaggggagac tgtaaagttt agtatatgaa aaaacaaata acacttataa gagacattca
      781 agaaattgag aaacagctta tccaagctga atcaggagtt ctttgcatac atctggcaaa
      841 tgaaaaactt atgcagtttg cctgcaactt tatttatctt gataaaaaca tctacacata
      901 tcttgatagc agtgacgaga attatgaaca tgttaaatat ggttccgcag gaagtttttc
      961 agtttcatca agcgaaaaat taacaggaaa attaaaagag ttttcctatc ttttatcctg
     1021 catcacaatt aatggtgaaa ttaaagaagt tgatgatagt aaacttaaag atcagatcgc
     1081 tgaattgtat cggctgaaat attcaccagg gataaaagct gataaatacg aagttatgga
     1141 aaatcttaaa cctgttattc tggatacgaa cgaaataaaa gctctgatcg aagaaggaaa
     1201 ttaaccctca ttcactaatt attcttctat aaataaggga gaattaactt gttaaattac
     1261 gtatggatct ctctggtatt cctcggactt gcctcggcac tttttataga tatatccgat
     1321 atcactcaaa atacttacaa caatgatatt ccacttactg ttacaataaa aacctctgcg
     1381 atcaaatcag gcggaggatt tgattgtgaa gtgaatatcc cgaaagaaaa ttataataaa
     1441 tttttcaaat cgaacgaatc agaaaacatt gttgtgcatg gagttggttc ttttagtgaa
     1501 gataaaaaag ctatccagtt atatctaaag acatcagaaa acgcaccgaa aaaacttcag
     1561 gttattgcaa aagcttcagg caaagatgat gatattcttg cacgtgcaaa tataatttct
     1621 aatcaggata gcattacata tctatctaaa gtttactttg aaaaaataag tttcctttcg
     1681 atgaagcgtg ttacaaatga ttcaattaat atcgctggta ctgcagttca aatagcattg
     1741 ggcttgattg gaataatggc gatgtggctt ggtgttatga aagttgcaga gcaggctgga
     1801 ttgataaaaa ttattgccaa ctggttaaag ccgataacta aaaaactttt tcctgaagtt
     1861 cccccggatc atccggcagt tggcgcgatg gttatgaata tatcggctaa tatgcttgga
     1921 ctcggaaacg ccgcaactcc ttttggatta aaagcaatgg aagaattgga tacattaaat
     1981 ccaaataagg gaactgcaac taactcaatg gttacttttt tagcaattaa tactgcgggc
     2041 atgacaatta ttcctgcaac tgcaatcgct gttcgtgctg cagccggaag cgctgatcca
     2101 accataataa ttgcaacatc attgttcggt tctacctgtg caactataac aggattaaca
     2161 gttgcaaaac tgttcgaaag atttccaata gagaaaaacg gatatggaag ctggtttaaa
     2221 agcaatctta aatttttaat ttcagtatta tctttaattg caatacttgt gttaacattt
     2281 gtcactggat tttcatctgt aattggttca gcttttaatt ttcttccgcc tacctttttt
     2341 aaagatatga ttcagatcgt atcaacatta gcaatcccgt tttttatttt tatttttatt
     2401 ggatatggtg taataaaaaa agttagagtt tatgaacagt ttgttgaagg tgcaaaagag
     2461 ggatttaaca ttgcaataag aataatacct taccttgttg ctatgcttgt tgcaattgca
     2521 atttttagag caggcggagc gatggataat tggttaattc ctgttttgcg ggttgttaca
     2581 gatcctattg gaatgcccgc agaagctctt ccaatggcgc tgatgaggcc attatccggc
     2641 agcggttcac ttggtgtgat ggctgatatt atagctgtcc acggaccaga ttcatttatt
     2701 ggaattttag tttcaacttt cttcggcagt tctgaaacta ctttttatgt actggcagtt
     2761 tactttggtg cagtaaatat aaaaaacact cgccacgctc ttgccgcagg attggctgcc
     2821 gatattgcag gtgcgcttgg tgctttgttt atagttaagc ttttgtttgg gtgagataat
     2881 tttctaagta t
//
LOCUS       DASWBY010000537         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_24727, whole genome shotgun sequence.
ACCESSION   DASWBY010000537 DASWBY010000000
VERSION     DASWBY010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24727"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1492
                     /gene="gatB"
                     /locus_tag="VF870_09670"
     CDS             56..1492
                     /gene="gatB"
                     /locus_tag="VF870_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX9252499.1"
                     /translation="MNTKYEAVIGLEVHTQLLTDSKIFCGCSTKFGNDPNSNTCPVCL
                     GHPGVLPVLNKKVVEYTALMGLATNCQINEHSVFARKNYFYPDLPKGYQISQYEKPIC
                     ENGFIEVPAKNGLTKKIGITRIHMEEDAGKSIHDQSSTTLVDVNRCGVPLMEIVSEPD
                     IRTPEEAYLYLSKIKQVVQYLGICDGNMEEGSLRCDANISVRLKGESNFGVKTEIKNM
                     NSFRNVERAIEYEINRQIELIEYGEKIKQQTLLWDADANEAHPMRSKEEAHDYRYFPD
                     PDLLPVIVYEEWKREIERSLPELPDAKLKRFVTDFSLPVYDSEILTQTKALAKYYEEV
                     VSVTDEYKIASNWVTGDVLAVLNDKKIDISDFPVSPLNLGRLINLIKKETISGKIAKE
                     IFPIMLENDSDPEQIVKEKNLVQISDTSEIETLIKRIIEANSGQVEEYRSGKEKVFGF
                     FVGQVMKESKGKANPKLVNEILRKMLSS"
BASE COUNT          636 a          253 c          330 g          491 t
ORIGIN      
        1 ctttctatat aaagattatc atccatatat ttagttgcga aaagaatcac attttatgaa
       61 tacaaaatac gaagcggtaa taggtttaga agttcacacc cagcttttaa cagactcaaa
      121 aattttttgc ggctgttcaa ctaagtttgg taatgatccc aacagcaata cttgtccggt
      181 gtgcttaggt catcccggag ttttgcctgt acttaataaa aaagttgtcg aatatacagc
      241 tcttatggga ttggctacta attgccagat aaacgagcat tctgtatttg caagaaaaaa
      301 ttatttctat cccgatcttc caaaaggata tcaaatctct cagtacgaaa aaccgatttg
      361 tgaaaacggt tttatagaag tccccgctaa aaatggtttg actaaaaaaa tcgggattac
      421 aagaatccat atggaagaag atgccggcaa atctattcac gatcaaagca gtaccacgct
      481 tgttgatgtg aacagatgcg gagtcccact aatggaaata gttagtgagc ctgatatccg
      541 aactcctgaa gaggcctatc tttatttaag taagattaaa caagtagttc agtatcttgg
      601 aatttgtgat ggaaatatgg aagaaggctc attaagatgt gatgcaaata tttctgtaag
      661 gttaaaaggt gaaagcaatt ttggtgttaa aactgaaatt aaaaatatga actcatttag
      721 aaatgttgag cgggcaattg agtacgaaat aaatagacag atagaattga ttgaatatgg
      781 agaaaaaatc aagcagcaaa ccttactttg ggatgctgat gcaaacgagg ctcatcctat
      841 gagaagcaaa gaagaagcgc acgattacag atatttccct gatccggatt tgcttcctgt
      901 aattgtatat gaagaatgga aaagagaaat tgaaagatca cttcccgaac taccggatgc
      961 caagttaaaa agatttgtta ctgatttttc acttcctgtt tatgattctg aaatacttac
     1021 acaaactaaa gcacttgcaa agtattatga agaagttgta tctgtaactg atgaatacaa
     1081 aattgcaagc aactgggtaa caggtgatgt tcttgcagtt ttaaatgata aaaagataga
     1141 catttcggat ttccctgtgt cacccttaaa tttgggccgg ctaatcaatc ttattaaaaa
     1201 agaaaccatt agcggcaaga ttgccaaaga aatatttccg ataatgctgg aaaatgattc
     1261 tgatcctgaa caaattgtaa aagaaaaaaa ccttgttcag atttcggaca catcagagat
     1321 tgagacatta atcaaaagaa ttattgaagc aaattcagga caagttgaag aatatcgatc
     1381 aggcaaagaa aaagtatttg gattttttgt aggacaggtt atgaaagaat caaaaggcaa
     1441 agcaaatcca aagcttgtta atgagatttt aagaaaaatg ttatcatcgt agcgcagaat
     1501 ttgttatgcg aatgatttta taaactagtt tagagaaaac acagacttag gtttgtgcta
     1561 cggaatttta attacatata gtatggagaa ataaattgtc atttcaggaa aaagtaaaga
     1621 acttttttaa gaaaaaagaa aagccaaaac caactacaat aagtggtaaa attgcagagt
     1681 tctttaaaca gttagcattt gcaggaattg
//
LOCUS       DASWBY010000538         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_56276, whole genome shotgun sequence.
ACCESSION   DASWBY010000538 DASWBY010000000
VERSION     DASWBY010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56276"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="VF870_09675"
     CDS             complement(<1..1716)
                     /locus_tag="VF870_09675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9252500.1"
                     /translation="MDLSIIIVNYNVKEFLQNLVHSIEKASSNLTKEIIIVDNASDDD
                     SVDFIKEKFPQVKLIANQTNLGFGKANNIGLNQATGKYILLINPDTLVAEDTFEKMIK
                     FFESNLSAGLAGCKILNPDGTLQLACRRSFPGPWTSFTKVIGLSNLFPNSKIFARYNL
                     TYLDENKSYEVDAISGSFMMMRKEVYQKVGGFDEQFFMYGEDLDLCYRIQKAGFKVFY
                     VHYTQIIHYKGESTKRSSIDETKVFYSAMHLFVKKHLSSSLLVGLILRSAIFVRSIFA
                     FIGVRKLIILSVILDFIFFDLCLFAAEKIYHNVSDWKGFPGFAIPIVYSIPALIQVFV
                     SFAIGNYKKDKISVSKTLLSILMSLPILTSLTFFFKDFAFSRAIVLITYSFLFIVLAA
                     WRIVFKIVFKTGLVDDELIQKRTLIVGLDESAIQIGQKIRKKKTDRRSVVGLIGFSNK
                     NIGDKIESFEVIGTDQNIKRVIRDNKINEIIFSSGELSYNKMMEIVGKCREENVEFKI
                     VGNNLDFIVGKTAVTMLDDMPVIELNYNITQTQQRLIKALFDYSIVIPSLFLIYPFIF
                     FKSKLS"
     gene            complement(1728..2450)
                     /locus_tag="VF870_09680"
     CDS             complement(1728..2450)
                     /locus_tag="VF870_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX9252501.1"
                     /translation="MSNLKSLVIIPTYNELENLPRMIPAVLSQDQSIHLLIVDDGSPD
                     GTGAFVKEEMKKNDRIHILEREKKMGLGTAYIAGFKYALQNNYDFIFEMDADFSHDPN
                     ELKNFLIAIKENDLVLGSRYINGVRVLNWPMSRLLLSFFASVYTRIIIGMPIKDATGG
                     FKCFRRSVLEAIDLNKVRSNGYSFQIEMTFKAFSQGFKIKEIPIIFIDRVKGKSKMSK
                     KIVREAVVMVWKLRFQRMFGKL"
     gene            complement(2499..2855)
                     /locus_tag="VF870_09685"
     CDS             complement(2499..2855)
                     /locus_tag="VF870_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9252502.1"
                     /translation="MKSKFRFQDLDIWKQAIEIADELFDIADTLEKNRLFRFSEQLRG
                     AAMSMSNNIAEGSGSEWKKEFKNFLNIARRSAFENANILIILNRRKLLNDDIVERLLD
                     KLDKECRMITNFKKTL"
     gene            complement(2936..>3109)
                     /locus_tag="VF870_09690"
     CDS             complement(2936..>3109)
                     /locus_tag="VF870_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX9252503.1"
                     /translation="GVTGWAQIKHKYDETIDDVKIKLRYDLFYIENMSIRMDFKILLR
                     TVFVVLFGKGHYD"
BASE COUNT         1040 a          570 c          410 g         1089 t
ORIGIN      
        1 tgatagttta cttttgaaaa atatgaaagg ataaatcaaa aacaaagaag gaataactat
       61 tgagtaatca aataatgctt ttattaacct ttgctgagtt tgagttatat tataatttag
      121 ttcaattaca ggcatatcat ccagcatagt aactgcggtt ttaccaacta taaaatcaag
      181 gttatttcca actattttaa actcaacatt ttcttcccgg cactttccaa caatttccat
      241 cattttgtta taggataatt caccggatga gaaaataatc tcgttaattt tattatctct
      301 aattacgcgt tttatgtttt gatcagttcc gattacctca aaagattcaa ttttatcccc
      361 gatgttttta ttagaaaatc caattaatcc tactacactt cttctatctg tttttttctt
      421 tcttatcttt tgtccaattt gaattgcgct ttcatccaga ccaacaataa gtgttctctt
      481 ttgtataagc tcatcatcta ccaaaccggt tttaaataca attttaaaca caattcgcca
      541 ggcagcaagt acaataaata aaaacgagta cgttatcaaa acaattgcac gactaaatgc
      601 aaaatcttta aagaaaaatg ttaatgatgt aagtattggc aggctcatta atatcgacag
      661 caaagttttt gaaactgaaa ttttgtcttt cttatagttc ccaatcgcaa atgaaacaaa
      721 aacctgtatt agagccggta tggaataaac aattggtatt gcaaaaccag gaaatccttt
      781 ccaatcgctt acgttatgat aaatcttttc agccgcaaac aaacagagat caaagaaaat
      841 aaaatcaaga ataacagaaa gtataataag tttacgcaca ccaataaaag cgaagatact
      901 tcttacaaaa attgcactcc gtaaaatcag tcctacaagc aaagagcttg ataaatgttt
      961 ttttacaaac aagtgcatcg cactataaaa aacttttgtt tcatcaatgc tgctgcgctt
     1021 agtactttct cccttgtagt gaataatttg agtatagtga acataaaaga ctttaaatcc
     1081 agctttctga attctatagc ataaatccag atcttcacca tacataaaaa actgttcatc
     1141 aaatccaccg accttttgat aaacttcttt tcgcatcatc ataaaagatc ctgagatggc
     1201 atcaacctca tagcttttat tttcatcaag ataggtgaga ttatagcggg caaatatttt
     1261 actattcgga aacagattac ttaacccgat tacttttgta aatgatgtcc acggaccggg
     1321 aaaacttctt ctgcaggcaa gctgcaaagt accgtccgga tttaaaattt tacatcctgc
     1381 taatcctgct gataaatttg actcaaaaaa tttaatcatt ttttcaaaag tatcttcagc
     1441 aacaagtgta tcgggattaa tcaataaaat gtatttaccc gttgcttggt ttaagccgat
     1501 gttatttgct ttaccgaatc caagattggt ttgattagca attaatttta cttgcggaaa
     1561 tttttcttta ataaaatcaa cgctgtcatc atcggatgcg ttatcaacta taataatttc
     1621 tttggtaaga tttgatgagg ctttttcaat tgagtgaact aaattctgaa ggaattcttt
     1681 tacgttatag tttactatga ttattgaaag atccaattat atgatcctta caatttgcca
     1741 aacatacgct gaaaccgaag tttccaaacc ataactactg cttcacgaac aatttttttg
     1801 gacatttttg attttccttt gacgcgatcg ataaagatga ttggaatttc tttgatttta
     1861 aatccttgag aaaatgcttt aaatgtcatt tcaatctgaa atgaataccc gtttgatctg
     1921 actttattta agtcaattgc ttccagtaca cttcttctaa aacatttaaa tccaccggtt
     1981 gcatctttta tcggcattcc gattattatt cgtgtgtaaa cactggcaaa aaaacttaac
     2041 aataaccttg acataggcca attaagtaca cgaacaccat ttatatatct gctgccaagg
     2101 accagatcat tttctttaat tgctattaaa aagtttttta gttcattagg atcatgagaa
     2161 aaatccgcat ccatttcaaa aataaaatca taattattct gcaaagcata tttaaatccc
     2221 gcaatgtaag ctgtacccaa acccattttc ttttcacgtt caagaatatg aatgcgatca
     2281 ttttttttca tttcctcttt tacaaatgct cctgttccat caggagagcc atcatcaaca
     2341 ataagtaaat gaatgctttg atcctgtgat agaacagcag gaatcattct gggaagattt
     2401 tccagctcgt tgtatgttgg gattattact aaagatttaa ggttgctcat agtttttgca
     2461 tagggcatag cgcagtgcgc atagcaaatt aaaataattt ataatgtttt tttaaaatta
     2521 gttatcattc tgcattcttt atcaagttta tctaaaagtc tttcaacaat atcgtcattt
     2581 aataattttc ttctatttag tattattaaa atatttgcat tttcaaaagc tgatcttctc
     2641 gcaatattta aaaaattctt aaactctttt ttccattcag aacctgaacc ttcagctata
     2701 ttattcgaca tactcattgc agcaccacgc aattgttctg aaaacctaaa taagcgattt
     2761 ttctcaagtg tatcagcaat atcaaataac tcgtctgcaa tttcaatagc ctgtttccaa
     2821 atatctaaat cctgaaatct aaattttgat ttcataaaat catcattaac tttattttat
     2881 ctttacgctt tgttcttagg atttactcta tgccctttgc acttagcgct atgcgctaat
     2941 cataatgccc tttgccaaat aatactacaa aaacagtacg cagcaagatt ttaaaatcca
     3001 ttctgattga catattttca atataaaaaa gatcgtaacg taatttgatt tttacatcat
     3061 caattgtttc atcatactta tgttttatct gagcccaacc tgttactcc
//
LOCUS       DASWBY010000539         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_618164, whole genome shotgun sequence.
ACCESSION   DASWBY010000539 DASWBY010000000
VERSION     DASWBY010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618164"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VF870_09695"
     CDS             <1..547
                     /locus_tag="VF870_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252504.1"
                     /translation="YFTKEIRQLIVKAGDEVDEESLRLQARKDGSLTLREAGLEKVKM
                     GIEPFLIAYAINLIVAQRLVRKLCTKCRKRVVNFDEGIMMAAELNIAEWRNYTIYEAA
                     GCDECNGTGYKGRLAIHEALYFTKEIRQLIVKAGDEVDEESLRLQARKDGSLTLREAG
                     LEKVKMGLTSIEEVLASTSES"
     gene            693..1919
                     /locus_tag="VF870_09700"
     CDS             693..1919
                     /locus_tag="VF870_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX9252505.1"
                     /translation="MNIAEEKKILESFCKTIPASIFGPDRVHYILDNIDKLKENERVL
                     LRKFYNKLLTNMIEKEASDIEIGGHGNVGYVWMRIYGNKERVKEIGQFTTDESAVIII
                     NLLNVNQRDQLAKKRNLDFSYTFLYERRNANVRFRSDAYFDLDTVTLNMRAINQVIRP
                     LSSLDFHPNIVRALSHGYIKFGLSLITGITGSGKSTTLDSIIDHHNKIDPCHIIIVAA
                     PVEFVHTSNVSLIKHREVGRDVNSFKEGIVQALRQDPDIIVVGEMRDPETIMAALEVT
                     DTGHKVFSTLHTSSAVESIDRIIAECDPAEQERVRNRLADVLISIVSQKLVPSLDGKR
                     VMAKEVLLVTPSVKAAIKNNNTSEIYMMINQGGAQGMITMEQDLKRLYLAKKISLETA
                     ITYANNKTRIQQILAA"
     gene            1923..>2391
                     /locus_tag="VF870_09705"
     CDS             1923..>2391
                     /locus_tag="VF870_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252506.1"
                     /translation="MKTIACIYCEGNDTKLAVVNQEKIGSRLQIMSTASVDVVSSQSN
                     LQPTAGFNLEGEGLQLENVDTKETSLAGDIDAPSISAMGAALKGVNLNKLEFIPALTE
                     PAIYYHSYDGKKDLKGSKLLDEILSDIRQSKGVNVEKESVDYIELADGSLLSAF"
BASE COUNT          837 a          353 c          497 g          704 t
ORIGIN      
        1 ttattttaca aaggaaataa gacagcttat tgtaaaagcg ggcgatgagg ttgatgaaga
       61 aagtttaaga ctacaggcac gtaaggatgg ttcattaaca ttaagggaag ctggtttgga
      121 aaaagttaag atgggtatag agcctttttt gatcgcttat gcaattaatc tcatcgtggc
      181 acagaggttg gttagaaaac tgtgcacaaa gtgcagaaaa agggtggtaa attttgatga
      241 aggtattatg atggccgcag aattaaacat tgcggagtgg agaaattaca caatttatga
      301 agctgccgga tgcgatgaat gtaacggaac cggatacaaa ggaaggcttg caatacacga
      361 agctctttat tttacaaagg aaataagaca gcttattgta aaagcaggtg atgaagttga
      421 tgaagaaagc ttaagactac aggcacgtaa agatggttca ttaacattaa gggaagctgg
      481 tttggaaaaa gttaaaatgg gtttaacttc aattgaagaa gtgttagcaa gcaccagcga
      541 atcgtaaaat tttttactga actgtaattt ttacttaaca tttatatcaa aaggatataa
      601 atattacatt tttaatcaaa aaacagtttc ttatgtgctg gtatagtttt atctaacaca
      661 tagcggaaca ttaaatttat tgaaggttta aattgaacat tgcagaagag aaaaaaatat
      721 tagaaagttt ttgcaaaact attcctgcat caatttttgg cccggataga gtacattata
      781 ttcttgataa cattgataag ttaaaagaaa acgaacgtgt tttgcttaga aagttctata
      841 ataagcttct taccaatatg attgagaagg aagcttctga tattgaaatt ggcgggcatg
      901 gtaatgttgg ttatgtctgg atgcgtattt acggcaacaa agaacgtgtt aaggaaattg
      961 gtcagtttac tacagacgag tcggctgtaa ttatcattaa tcttcttaat gttaatcaaa
     1021 gagatcaact cgcaaagaaa cgaaatttag acttcagcta tacattcctt tatgaaagaa
     1081 gaaatgcaaa tgtaagattc agatccgatg cttattttga tctggatact gtaacgttga
     1141 acatgagagc aataaatcag gtaataagac cattgtcttc tcttgatttt catccgaata
     1201 ttgttcgtgc attaagtcac ggttatatta aatttggttt atcacttatt acaggaatta
     1261 ctggttccgg taaatctaca actttagact ccattattga tcatcataat aaaattgatc
     1321 cctgccatat tataatagtt gctgcgcctg tagaatttgt tcacacttct aatgtatcat
     1381 tgattaagca cagagaagtc ggaagagatg tgaattcatt caaagaaggt atcgttcagg
     1441 cactgcgtca ggatcccgat attattgttg tgggtgaaat gagagatccg gaaacaataa
     1501 tggcggctct agaagtaact gataccggac ataaagtttt ttctactctt cacacatctt
     1561 ctgctgtaga atctatagat agaataattg cagagtgtga tcctgcagaa caggaaagag
     1621 taagaaacag acttgccgac gttttaattt ctatagtatc acaaaaactt gttccaagtc
     1681 tagatggaaa acgtgtaatg gcaaaagaag ttttacttgt tactccaagc gtaaaagctg
     1741 caattaaaaa taataacaca agtgaaattt atatgatgat taatcagggc ggtgcacagg
     1801 gaatgataac tatggaacaa gacctaaaac gtttgtatct tgcaaagaaa atttcattag
     1861 aaacggcaat tacttatgcg aataataaaa caagaattca gcaaatactg gcggcctaaa
     1921 ttatgaaaac aatagcctgc atatattgtg agggtaacga caccaaactt gctgtcgtta
     1981 accaggaaaa aattggaagc agattacaga ttatgagtac tgcatctgtt gatgtggtct
     2041 catcacaatc taatttgcag ccaacggcgg gatttaatct ggaaggtgaa ggacttcaac
     2101 tggaaaatgt tgatactaaa gaaacttcac ttgccggaga tattgacgca ccaagcatca
     2161 gcgctatggg tgcggcatta aaaggagtaa atcttaataa actggaattt attcctgcat
     2221 taacagaacc cgcaatttat tatcactcct atgatggaaa gaaggattta aagggcagta
     2281 aactgctcga tgaaattctt tctgatatcc ggcaatctaa gggtgtaaac gtagaaaagg
     2341 aaagcgtgga ttatattgag cttgcagatg gctccttatt atctgctttt c
//
LOCUS       DASWBY010000540         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_283420, whole genome shotgun sequence.
ACCESSION   DASWBY010000540 DASWBY010000000
VERSION     DASWBY010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283420"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VF870_09710"
     CDS             <1..828
                     /locus_tag="VF870_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9252507.1"
                     /translation="VKTNYGYHIIKVTDRSNKKYIVEKIVNQVKQSATSRDRNFNAAN
                     DFAFLAKKNDFESEAKLLNYEVLETPLFSELSGSVPSIGQNKRLVKFSFENSLNTISD
                     PFKVPAGYVVVKISEVAAEKFRPFEELKEQLKPAVLREKKFEKIKTIAEDLYKKIGGD
                     LSKAASIDTNFVVKQTGNFTPQGSVPNVGRDYAFINTALKADLNKVSEPVKGLRGYYL
                     IKVTKRTPFDSTLYATQSTTLRNTMLQEKRSRFLNQWVTEIKNSADIKDNRYMFFGQ"
     gene            965..1519
                     /locus_tag="VF870_09715"
     CDS             965..1519
                     /locus_tag="VF870_09715"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX9252508.1"
                     /translation="MKKKLVYLTGFMASGKSTIGPILANTLGWNFVDLDKVIEENAGK
                     SVRTIFEEYGEDYFRELETETLKKNSELENYIIALGGGTIANQKNLEIIKSSGFLIYL
                     ESSPEETYKRLRFKRDRPALLLDGVEEPNKVEFIEKINSLLLKRIYYYNQADVKINTD
                     NYKVGKTVDKLSQIIKKEFYGENN"
     gene            1503..2594
                     /gene="aroB"
                     /locus_tag="VF870_09720"
     CDS             1503..2594
                     /gene="aroB"
                     /locus_tag="VF870_09720"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX9252509.1"
                     /translation="MAKIINVKLLDKNYPIIIGNDSLLKAVEKINKLSPSKCLLITDK
                     NVLKYHSLLIRKTFAQLDCKVYNHTFSAAEKNKSLNQTEKIYKYLTDNCFDRNSIIVA
                     LGGGITGDIAGYAASTFMRGIKFIQIPTTLLSMVDSSVGGKTGVNFKNQKNVIGTFYQ
                     PEFVTVYPEFLLTLPKRELQSGAGELFKYSFLADNKNYYLLKNSLEKLFANESFNIEK
                     TIYACLKIKSSIVENDEKEITGLRKILNLGHTFAHAFEVESNYKLKHGEAVIGGIFCA
                     LFLSEILGYMTSEKLNIIIKDFRFIKPHKILASLNPDSVIKAMGSDKKNSSGKIRFVT
                     VEDNGSIIIDVDSDKSFVLEAVERMKRLV"
     gene            2825..>3289
                     /locus_tag="VF870_09725"
     CDS             2825..>3289
                     /locus_tag="VF870_09725"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HEX9252510.1"
                     /translation="MQTEFSSSGVNLKAQIKLLKEKLNAVILAHYYQESEIQDIADFV
                     GDSLELAKKAKSTSADVIVFAGVHFMAETAKILNPDKLVLLPDLEAGCSLAEGCPADE
                     FKFFREKHPDHLAITYINCSADVKALSDIICTSSSAEKIINQIPKDQPILFSP"
BASE COUNT         1191 a          444 c          578 g         1076 t
ORIGIN      
        1 gtaaaaacta attatggcta tcacattata aaagtaaccg atagatcaaa taaaaagtat
       61 atagttgaaa aaattgtaaa tcaggttaaa caatcagcta ccagcagaga tagaaacttt
      121 aatgctgcta atgattttgc atttcttgca aagaaaaatg atttcgagtc tgaagctaaa
      181 ttgctgaact acgaagttct tgaaactcca cttttctcag agttatcagg ttcagttcct
      241 tcaataggac aaaacaaaag attggttaag ttttcatttg agaatagttt gaataccata
      301 agtgatcctt ttaaagttcc agcaggatat gtagttgtta aaatttcaga agttgctgct
      361 gaaaaattcc gaccttttga ggaactgaaa gaacaattaa aacccgcagt tcttagagaa
      421 aagaaatttg aaaagataaa aacaattgct gaagatcttt acaagaaaat tggcggtgat
      481 cttagcaaag ctgcttcgat agatactaat tttgttgtta agcagactgg aaactttacg
      541 cctcaaggtt cggttccaaa tgtaggcagg gattacgctt tcataaatac tgctttgaaa
      601 gcagatttaa ataaagtatc agagcctgtt aaaggattaa gaggttatta tctcataaaa
      661 gtcactaaaa gaacgccatt tgacagcact ttgtatgcaa cacaaagtac aactttgcgt
      721 aatacaatgc ttcaggaaaa aagaagccgc ttcttaaatc agtgggtaac tgagattaag
      781 aacagcgcag atattaaaga taacagatat atgttttttg gtcagtaatc ctaattaaga
      841 aattttcaac ccccaatcac acttaattgc tgttgttggg ggttttcttt ttcatgtaat
      901 tcatttcttt tttttctatt tcaatttctt aaattaaccc ataaagattt acttaatatt
      961 tggtatgaaa aaaaaattag tatacttaac tggatttatg gcttcaggta aaagcaccat
     1021 tggacctatt cttgccaaca cgcttggttg gaattttgtt gatctggata aagtgattga
     1081 agaaaatgcg gggaaatctg ttagaactat ttttgaggag tatggagaag attattttag
     1141 agaattggaa acagaaacac ttaaaaaaaa cagtgaatta gaaaattaca tcatcgcact
     1201 tggcggcggc acgattgcaa atcaaaaaaa tcttgagata attaaatcat ccggttttct
     1261 tatttatctt gaatcgtctc ctgaagaaac atataaaaga ctaagattta aacgagacag
     1321 acctgcgtta cttttagatg gagtggaaga gcctaataaa gtggaattta ttgaaaaaat
     1381 taattctctt ttactaaaaa ggatttatta ttataatcaa gcagatgtta aaataaatac
     1441 agataactac aaagttggaa aaactgttga taaactatca caaattataa aaaaagaatt
     1501 ttatggcgaa aataattaat gtaaaactat tagataaaaa ttatccaatt attattggta
     1561 acgattcttt attaaaagct gttgaaaaaa taaataagtt gtctccatca aaatgtttgc
     1621 ttatcactga taaaaatgtt ttaaagtatc attctttgtt aattagaaaa acctttgctc
     1681 agcttgattg caaagtttac aatcatactt tttctgctgc tgaaaaaaat aagagtttaa
     1741 atcaaacaga aaagatttat aaatatttaa ccgataattg ttttgacaga aattcaatca
     1801 tagtagcgtt aggaggagga attacaggtg acatagcagg ttatgcggca agtacgttta
     1861 tgcgcggaat aaagttcatt caaattccaa caactttgct ttctatggtg gatagttctg
     1921 tcggtggaaa aacaggtgta aattttaaaa atcaaaaaaa tgtaattggt actttttatc
     1981 aacccgaatt tgtaactgtt taccctgaat ttcttttaac gctacctaaa agggaattac
     2041 aatcaggtgc aggtgaatta tttaaatact catttttagc tgataataaa aattattatt
     2101 tgttaaaaaa cagcttggaa aaattattcg caaatgaatc ttttaacatt gagaaaacca
     2161 tttatgcatg tttaaaaatc aaatcaagta ttgtagagaa tgatgaaaag gaaattacgg
     2221 gacttcgcaa aattctaaat ctgggccata cttttgcgca tgcttttgaa gtagaatcaa
     2281 attataaatt aaaacatggt gaagccgtaa ttggtggtat tttttgtgct ttatttcttt
     2341 ctgagatttt aggatatatg acaagtgaaa aacttaatat tataattaaa gattttcgat
     2401 ttattaaacc gcataaaatt cttgcttctc taaatccgga ttcagttata aaagcaatgg
     2461 gaagtgataa aaaaaattct tctggaaaaa ttagatttgt tacggtagaa gataatggca
     2521 gcattattat tgatgtggat tctgacaaat cttttgtttt agaagcagtt gaaagaatga
     2581 aaagattggt ataaatacaa aacctcttta aaagaggaaa ctgtcaggtt ttgagggcga
     2641 aacctgacag tttgtgtgtc atttttggag ggatgctatg cttaaactat ttcggagaat
     2701 acttaacaac atcggtgcca tctaattttc tgtctaatga caattttggg cacaatttgg
     2761 acggtaaaaa atgcgtttta gtaattttta cttatggtaa tttttagaat gattggagat
     2821 ttttatgcaa actgaattta gtagtagcgg ggttaatttg aaagctcaaa taaaactact
     2881 taaagaaaaa ctaaatgctg taatacttgc acattactat caggaatctg aaattcagga
     2941 tattgctgat tttgttggag atagtttaga acttgcaaaa aaagccaagt ctacttctgc
     3001 agatgtaatc gtttttgctg gtgttcactt tatggcggaa acagcaaaga tattgaatcc
     3061 agataaatta gttttgctgc ctgatttaga agctggctgc tctttagctg agggttgccc
     3121 ggcagatgaa tttaaatttt ttcgtgaaaa gcatccagat catcttgcaa taacatatat
     3181 taattgttct gcagatgtaa aagcattgag tgatattatt tgtacttcca gcagtgctga
     3241 aaaaattatt aatcagattc caaaagatca gccaatactt ttttctccc
//
LOCUS       DASWBY010000541         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_475994, whole genome shotgun sequence.
ACCESSION   DASWBY010000541 DASWBY010000000
VERSION     DASWBY010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475994"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VF870_09730"
     CDS             <1..674
                     /locus_tag="VF870_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9252511.1"
                     /translation="IDDIALIIIEPIQAEGGDNFFRKEFFIKLREIADKNEILLMFDE
                     VQTGFGLTGKFWASDYYVKPDIIAFGKKAQVCGIMVSDRIDDIPDNVFKVSSRINSTW
                     GADLTDMVRSKFILDVIKNENLVDNAKKVGEHLKFQLLKIQEEFPSLINNVRGLGLMC
                     SFDLPTAEIRNNFKDNCYEEKLMILGCGEKSIRFRPALNVTENDIDKGLTIIRKVLSL
                     MTSNN"
     gene            746..1423
                     /gene="rsmI"
                     /locus_tag="VF870_09735"
     CDS             746..1423
                     /gene="rsmI"
                     /locus_tag="VF870_09735"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX9252512.1"
                     /translation="MKLYIVSTPIGNLKDITLRAIETLKEVDFILCEDTRTSGNLLNH
                     LEIKKELISLNAFNEKNKIEFIVNRILSNQTAALISDAGTPLISDPGVRLVSACIEKQ
                     IEIIPIPGVSALIAGLSMSGLPTDAFVFEGFLPQKKGRQTKLKELAEEKRTIVLYEST
                     YRIEKLLKELNEFLPNRFVVVCREITKKFEETWRGFPKDILETLDNKTIKGEFVVVIA
                     PLDWVVK"
     gene            complement(1458..>1891)
                     /locus_tag="VF870_09740"
     CDS             complement(1458..>1891)
                     /locus_tag="VF870_09740"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX9252513.1"
                     /translation="AFNYTLNQLLEFTKGKNINKVVGIESRGFIFGSVLSHKLGCGFI
                     PVRKPGKLPAEKVSISYSLEYGEDKLEIHKDAVQAGDRILVHDDLLATGGTMNAVCQL
                     IEKLGGEIVQVSFIVELSFLNGRDKLKSYDVRSIVNYENEE"
BASE COUNT          655 a          292 c          336 g          608 t
ORIGIN      
        1 atattgatga tatagcccta attattattg agccaatcca ggcagaaggg ggcgataatt
       61 ttttccgtaa agaatttttt atcaagcttc gtgagattgc tgataaaaat gagattctgc
      121 ttatgttcga tgaagttcaa acaggttttg gattaacagg aaaattttgg gcgtctgatt
      181 attatgttaa accggatata attgcattcg gtaaaaaagc acaggtttgt gggatcatgg
      241 tttccgacag aatcgatgat atccctgata atgtttttaa agtttcaagc cgtatcaact
      301 ctacctgggg tgctgatcta actgatatgg ttcgctctaa atttattctt gatgttatta
      361 agaatgagaa tcttgttgat aatgctaaaa aagttggcga gcatcttaaa tttcagcttt
      421 taaaaattca ggaagaattt ccaagtctta ttaataatgt acgtggactc ggattaatgt
      481 gttcattcga tttaccgaca gcagaaataa gaaataattt taaagataat tgttatgaag
      541 aaaaacttat gattttagga tgcggtgaaa aatcaatcag atttagacca gcattaaacg
      601 ttacagaaaa tgatattgat aagggcttga ctattatcag aaaagtttta agtttaatga
      661 cgtccaataa ctaaaatata cattaactac ttgcacggtg cggctccttt gttggagccg
      721 taactgtttt aaactatttt taactatgaa actttacatt gttagcacac caatcggaaa
      781 tcttaaagat attacattgc gtgctataga aacacttaaa gaagttgatt ttattttatg
      841 tgaagacaca cgtacatccg gtaatcttct aaaccattta gagattaaaa aggaattgat
      901 ttcattaaat gcatttaatg aaaagaataa aattgaattt atagttaata gaattttatc
      961 caatcaaact gctgcattga tttctgacgc tggaacaccg ctaatttctg atcccggggt
     1021 ccgactagtt tctgcttgta ttgaaaagca aattgaaatt attccaattc caggagtatc
     1081 tgcattaatc gccggattaa gtatgagtgg tttaccaact gatgcatttg tattcgaagg
     1141 atttcttccg caaaaaaaag gaagacagac taaactgaaa gagttagccg aagaaaaacg
     1201 aacaattgtt ctttatgaat caacttatag aattgaaaaa ttattgaaag agttaaatga
     1261 gtttttgccg aatagatttg tagtggtttg cagggagata acaaaaaagt ttgaagaaac
     1321 ctggcgcggc tttccaaaag atattttaga aacattagat aataaaacaa ttaaaggaga
     1381 gtttgttgtt gttatcgcac ctttagattg ggttgttaaa taaaaactat ttacagctca
     1441 aacactgaat cattaaatta ttcttcattc tcataattca cgatagagcg aacatcataa
     1501 gattttagtt tatcccggcc atttaagaaa gataattcca caataaatga aacctgaaca
     1561 atttctccac caagtttttc aattaactga caaactgcgt tcatcgtccc acctgtagca
     1621 agcagatcgt cgtgtaccaa gattcgatct ccagcctgaa cagcatcttt atgtatttcg
     1681 agtttgtcct caccatattc aagtgaatat gatattgaaa ctttttctgc aggaagttta
     1741 ccgggtttgc gaaccggaat aaaaccgcaa ccaagtttat gagaaagaac agaaccaaaa
     1801 ataaatccgc gagattcaat acctaccact ttattaatat tcttaccttt tgtgaattct
     1861 aaaagctgat taagtgtgta attaaaagct t
//
LOCUS       DASWBY010000542         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_700372, whole genome shotgun sequence.
ACCESSION   DASWBY010000542 DASWBY010000000
VERSION     DASWBY010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700372"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(63..204)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            361..1335
                     /gene="rfaD"
                     /locus_tag="VF870_09745"
     CDS             361..1335
                     /gene="rfaD"
                     /locus_tag="VF870_09745"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEX9252514.1"
                     /translation="MIVVTGGAGFIGSAIVWKLNQLGKSNIIIVDELGKDDKWKNLVG
                     LKYEDFANKLEFIEQILDDVIPYNVEAIIHMCANSSTTEKDADHLMDNNYNYTKELAK
                     YCVEKNIRFIYASSAATYGDGSLGFDDDESKLETLRPLNMYGYSKQLFDLWAKRNGIS
                     DQVVGIKYFNVYGPNENHKGDMRSVVHKAFEQVRDDGKVRLFKSLHPNYKDGEQMRDF
                     IYVKDAVDMTLYFLDKPDKNGIFNVGAGKARTWNDLVSSLFKAVGKPVNIEYIDLPSH
                     LAEKYQYFTEANLTKIRNAGYKQATTSLEDGVTDYVKNYLLNGSYLGY"
     gene            complement(1402..1707)
                     /locus_tag="VF870_09750"
     CDS             complement(1402..1707)
                     /locus_tag="VF870_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252515.1"
                     /translation="MEQNGLNLQTTEDNENPFAINNKVIFESLALFAAKKKNKNEDDD
                     YDDDDEEEKDDYYDDEEEDEDDLFKDEPKDKDDLEDEFDIDELDEDDLFDDDEEPTF"
     gene            1893..2357
                     /locus_tag="VF870_09755"
     CDS             1893..2357
                     /locus_tag="VF870_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEX9252516.1"
                     /translation="MKLATLCYVIDTKTNSTLMLHRVKKENDYHEGKWNGLGGKFEQG
                     ESPEECAIREIEEECGLKVKSVTMKGFITFPLFDGIDDWYVFLFTANEFEGNLIDSPE
                     GNLAWIKNDKLTELNLWDGDKVFIPWLFKDGFFSAKFNYENGKYVSHNVSFY"
     gene            2613..3866
                     /locus_tag="VF870_09760"
     CDS             2613..3866
                     /locus_tag="VF870_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9252517.1"
                     /translation="MKNLIFVLVLSVMFFSAQHTHAQLFDKIKKETEKVIQKETQKQP
                     EVKKETPPTNDEKNNTETKESTNTNKTSEETTSQKPQDNPQFSSSTQYDFVPGDKVIF
                     FDDFSQDAVGDFPALWTTNAPGEINTLSIAPGNWFNLNSTDGNYFILNDIAFPKNFII
                     EFDIVPKKTGGRIAAGLLLYGEDKHKEMDNNAHPGNGGIMISIEKENWNTWGYKTGEK
                     DLITGKSTVKPVVAEKVNHVIIWVQGRRLRIYHEQAKVLDMPTNIYDGVKLSRLCFRL
                     SRGASCGSFISNLRITDAAPDMRSKLLTEGKLVSYGIYFDVNKDIVKSESYGTLKEIS
                     KVLTDNPDVKIKIVGHTDSDGDDKSNLDLSKRRAASVKNVLVKDFGVDASRIETDGKG
                     EGAPVAKNDSAVNKALNRRVEFIKL"
     gene            3882..4322
                     /locus_tag="VF870_09765"
     CDS             3882..4322
                     /locus_tag="VF870_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252518.1"
                     /translation="MEIAFKAVSTIIMKNIYKHILWWFAFPFVGILNGVLREATYKKF
                     VGDLPAHQISTATGVIFFGIIFHFIFRKWKIESVKNAILIGVIWLCLTILFEFGFGHY
                     IMGNSWQKLLHDYNLAKGRVWGLFLVWITIAPFIFYKFFNKPKS"
BASE COUNT         1504 a          708 c          782 g         1383 t
ORIGIN      
        1 ctgccttctt taaaatgaaa aaacctctat caatgaaatg aagaggtttg caatttatct
       61 tcatctcatc tttccctccc gataaaattg ggaaggcagg atttagcacc cgacgttccg
      121 ataaacctgt ttgcaaacaa gtaaaaccga aaccggctgc tacggcatct gcgggcctgt
      181 tccctaagcc gttcttgata agaaatataa tttaaaaaga actaataaaa tctgacgtaa
      241 atatataaat tataactgct ttgtcaatga gatgctcatc aacttaaaac aggatgatac
      301 atatctttaa aactccgcta aatatggcta aattagttta taaaaaatca aaggatttct
      361 atgatagttg taaccggtgg tgcaggattt attggcagtg ccatagtctg gaagttaaac
      421 cagcttggca aatccaacat aataattgtt gatgagctgg gcaaagatga caaatggaaa
      481 aatcttgttg gattaaagta tgaagacttt gcaaataagc ttgaatttat tgaacaaata
      541 cttgacgatg ttattccata taatgttgaa gcaataattc atatgtgtgc aaattcttcc
      601 acaacagaaa aagatgctga tcatcttatg gataataact acaactacac aaaggagctc
      661 gctaagtact gtgttgaaaa aaacatccga tttatttacg catcctcagc ggcaacatat
      721 ggggatggtt ctttgggttt tgatgatgat gaatctaagc ttgaaacttt aagaccatta
      781 aatatgtacg gttactctaa acaattattc gatttatggg caaagaggaa tggcatttca
      841 gatcaggttg ttggaattaa atattttaat gtttatggtc cgaatgaaaa ccataaaggc
      901 gatatgcgtt cggttgtaca taaagcattt gaacaggttc gcgatgacgg aaaagttaga
      961 ttgtttaaat cacttcatcc aaattataaa gatggcgaac agatgcgtga ttttatttat
     1021 gttaaagacg ccgttgatat gactttatat tttttagaca agccggataa aaatggaatt
     1081 tttaatgttg gcgcaggtaa ggcaagaaca tggaatgatc ttgtttcttc tttgtttaaa
     1141 gcggttggaa aaccagttaa cattgaatat atagatttgc cttcacatct tgcagaaaag
     1201 tatcagtact ttacagaagc taatcttaca aaaatcagaa atgcgggata taaacaggct
     1261 acaacttcat tagaagacgg cgtaacagat tacgttaaaa attatctttt aaatggaagt
     1321 tatttgggat attgaaaaga gctaaataaa agtatgagtg agaggtcttt taatcctcac
     1381 tcatactctt aatcttaatc tttaaaatgt gggttcctcg tcgtcatcaa ataaatcatc
     1441 ttcgtctaat tcatcaatat caaattcatc ttctaaatca tctttgtcct tgggttcgtc
     1501 tttaaacaaa tcatcttcgt cttcttcctc gtcatcataa taatcatctt tttcctcttc
     1561 atcatcgtca tcataatcat catcttcatt tttgtttttc ttctttgcgg caaaaagagc
     1621 aagagattca aaaataactt tattattaat tgcaaacggg ttttcgttat cttctgttgt
     1681 ttgcaagttt aatccgttct gttccattat aaaatttctc cgataaaact taataattac
     1741 agtgttaatt tatatgagta tagcaaatct gcaaatagaa aaatacaaat ctttaaaaaa
     1801 tattttttct gattttttat caattagaac ttatattgct ttggtgtatt ttacaatagc
     1861 ataatttttt atatgaaaac ccaggacggt taatgaaact tgcaactctt tgttatgtga
     1921 ttgatacaaa aaccaactca acactaatgc ttcacagagt caaaaaagaa aatgattatc
     1981 acgaaggaaa atggaacggt ctgggtggca aatttgaaca gggtgaatct cccgaagaat
     2041 gcgccataag agaaattgaa gaagaatgcg gattaaaagt aaagtccgta acaatgaagg
     2101 gatttattac ctttcctttg tttgatggaa tagatgattg gtatgttttt ctttttaccg
     2161 ctaatgagtt tgaaggaaat ttaattgact cacccgaagg aaatcttgca tggattaaaa
     2221 acgataaact aaccgagcta aatctttggg atggtgataa ggtttttatt ccctggttat
     2281 ttaaagatgg tttctttagt gcaaagttta actatgaaaa tggtaaatat gttagccaca
     2341 atgtgagctt ttattgatat tccacttaac aaaaatcaat tgttggcaat tcacaaacat
     2401 tccgggtcct tgtacatttc ttactttgct ttctctcttt aaatattatt aagtttgatt
     2461 aatcaataat ttaaattatt aataaaacta acccagcaat tgtttcaggg agagataacg
     2521 tgccgcccac taaagaattc ctcaaagcag aacgctggct tctaaatatg taatggttta
     2581 aaataaattt taaatcaagt gaggagtgta ttatgaaaaa tctaattttc gttctcgtcc
     2641 tttctgtcat gtttttttct gcacaacaca cacacgccca actttttgat aagatcaaaa
     2701 aagaaactga aaaggtaatc cagaaggaga ctcaaaaaca acccgaagta aaaaaggaaa
     2761 ccccgcccac taatgacgaa aaaaacaata ctgaaacaaa agaatccacc aatacaaata
     2821 aaacaagtga agaaaccaca tcacaaaaac cccaggacaa tcctcagttc tcaagttcaa
     2881 cccagtatga ttttgttccc ggtgataaag taattttctt tgatgatttc agtcaggatg
     2941 ctgttggtga ttttcccgct ttatggacta caaatgcacc aggagaaatt aatactttga
     3001 gcatagctcc aggaaattgg tttaacctga acagcacaga tggaaattat tttattttaa
     3061 atgatattgc ctttccgaaa aactttataa ttgaatttga tatcgttcct aaaaaaacgg
     3121 gcggtagaat agctgccggg cttttgcttt atggagaaga taagcacaaa gaaatggata
     3181 ataacgcaca tcccggaaat ggcggcatta tgatctcgat tgaaaaagaa aattggaata
     3241 cctggggata taaaacagga gaaaaagatc tcatcaccgg aaaatctacg gttaaaccag
     3301 ttgtggcaga aaaagtaaat catgttatca tctgggtcca gggcagaagg cttagaattt
     3361 atcacgaaca ggctaaagtt ctggatatgc caacaaacat atatgacgga gttaagctta
     3421 gcagactttg ttttagacta agcaggggtg cttcctgcgg ttcatttata tctaatctta
     3481 gaattactga tgcagcaccg gatatgcgaa gcaagcttct taccgaaggg aagcttgtta
     3541 gttacggaat ttactttgac gtgaacaagg atattgtaaa atcagaatca tacggaacct
     3601 taaaagaaat ttctaaagtt ctaaccgata atcctgacgt aaaaataaaa atcgtgggtc
     3661 acacagattc cgatggtgat gataaatcaa acctggatct atccaaacgt cgtgcagctt
     3721 ctgttaaaaa tgttttagta aaagattttg gagttgatgc atcaagaatt gaaactgatg
     3781 gcaaaggtga gggtgcgcct gttgcaaaaa atgattcagc tgtaaataaa gcacttaata
     3841 gaagggttga atttataaaa ttgtaatgga ggttatttaa tgtggaaatc gctttcaagg
     3901 cggtctccac tatcatcatg aaaaacattt acaaacatat tctctggtgg tttgcttttc
     3961 cgtttgttgg aatattaaac ggtgtgctga gagaagccac ttacaaaaaa tttgttggcg
     4021 atcttccggc ccatcaaatt tcaacagcaa caggagttat tttctttggt ataatattcc
     4081 actttatttt tcgcaaatgg aaaattgaat ctgtaaaaaa tgcaattctt atcggcgtta
     4141 tctggctttg tttaacaatt ctttttgagt ttggatttgg acactacata atgggcaatt
     4201 cctggcaaaa acttttacac gattataatc ttgcaaaagg aagggtttgg ggtttatttt
     4261 tagtatggat aacaattgct ccttttattt tttataaatt ctttaacaaa ccaaaatctt
     4321 aatgcaaaaa gaactctgtg ctttctttcc aacgcaaaaa catttaagaa tttggtt
//
LOCUS       DASWBY010000543         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_25177, whole genome shotgun sequence.
ACCESSION   DASWBY010000543 DASWBY010000000
VERSION     DASWBY010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25177"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VF870_09770"
     CDS             complement(<1..438)
                     /locus_tag="VF870_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HEX9252519.1"
                     /translation="MKALITAFLLIIFSSQIIYSTDGQRLLSEPDPDIYKDKIVFTYE
                     NDLWLVNSNGGTATRLTSFPGNEFSAKFSPDGKSIAFTAGYDGTYSVYVMPSEGGEPV
                     RLTYTPSSAQSIAWTPDGKRIVFRSYWENYISRDPNLYFVNKEG"
     gene            complement(606..968)
                     /locus_tag="VF870_09775"
     CDS             complement(606..968)
                     /locus_tag="VF870_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9252520.1"
                     /translation="MPKEVLFIILGLAAGTFSGVIGLGGGVIIVPALVFLFGLTQQQA
                     QGTTLALMVPPIGILAAYAYYQQGYVDLKIAGLICLGFVVGGYIGAKFAVSLPTYILQ
                     KIFAISLFLIALKMFFAK"
     gene            complement(989..>1991)
                     /locus_tag="VF870_09780"
     CDS             complement(989..>1991)
                     /locus_tag="VF870_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9252521.1"
                     /translation="IPIVGNIFPFAEFGAKASAILCILFLTGINYVGVIFGGAVQSFV
                     TFIKIASIVILSFLLLASGSGSLANISTGFSMPASSTSNIISLFGLAIAGAFWAYDGW
                     NNLTYVAGEIKQPQRNVPLGLLFGTLIVVIVYMLINVAYLYVLPIEKMAQSPLVAATA
                     AEFVFGSKGAILISIAVIISTFGALNGNILSTARVCFAMARNNMFIKSLDKIHPRYAT
                     PHTSLVAQGLWSCLLVLSGTFDTITDYVMFASWLFYMLGAYGVIVLRKKMPDVPRPYK
                     VWGYPYTPMIFVIFSFLFLVNTLVSDYKNAAMGLLLILLGLPFYYWRIAKNNSIKKV"
BASE COUNT          719 a          369 c          322 g          581 t
ORIGIN      
        1 accttcctta ttcacaaaat ataaatttgg atcacgtgat atataatttt cccagtagga
       61 tcggaaaaca attcttttac catcaggagt ccaggcgatt gattgtgccg aacttggtgt
      121 gtaagttaat ctaaccggtt cgccgccttc ggaaggcatt acataaacgc tgtacgtacc
      181 atcatatcct gcagtaaatg caatcgattt accatcagga gaaaattttg cagagaattc
      241 gttgccggga aaactcgtta atcttgtagc agtacctccg ttagagttta caagccataa
      301 atcattttca taagtaaaaa caattttgtc tttatagata tcaggatcag gttcagacaa
      361 taatctttgc ccgtctgttg aataaattat ttgagacgag aagattatca gtaaaaatgc
      421 agtgatcaat gctttcatat tactttccta ataaaattaa tatttaaaat tacataaaat
      481 taaatttcaa aatggttgct aagatttcta tatattgatt caagatatct taatttcaca
      541 atctcagtta tctgtcctgg aataactcag cattgcaatc caaacaaata ataatctctc
      601 attaattact ttgcaaaaaa catttttaaa gcaattagaa aaagagaaat agcaaagatt
      661 ttttgaagaa tatacgtagg aagactaact gcaaattttg ccccaatata tccgccaaca
      721 acaaaaccta aacaaataag tcctgctatt tttagatcaa catatccttg ctgataataa
      781 gcgtatgcag caagtattcc tattggcgga accattagtg caagggtagt tccttgtgcc
      841 tgctgctgtg tcaatccaaa taaaaaaact aaagccggaa caataattac accgccgcct
      901 aatcctataa ctccgctaaa agtaccggca gctaaaccca gaatgataaa gagaacttct
      961 ttaggcatat aatcctcaaa ttattaaatc aaactttttt tattgaatta ttttttgcta
     1021 tccgccagta ataaaagggc agaccaagaa gaataagtaa taaacccatt gctgcatttt
     1081 tataatcgga aactaaagtg tttaccaaaa ataaaaatga aaagataaca aatatcattg
     1141 gtgtataagg atagccccat actttataag gtctgggaac atccggcatt ttttttctca
     1201 gtactataac accataagcc ccaagcatat aaaacaacca tgaagcaaac attacataat
     1261 ctgtaattgt atcaaaggtt cctgataata caagtaaaca cgaccataag ccctgcgcaa
     1321 ccagagacgt atgaggagtg gcatatcttg gatggatttt atcaagtgat ttgataaaca
     1381 tattgtttct tgccatcgcg aaacaaactc ttgctgtaga aagtatattt ccgttcaatg
     1441 ctccaaacgt agatataata actgcaattg aaattaatat tgctcccttt gatccgaaaa
     1501 caaattcagc tgcagtagct gcaacaagag gagattgggc cattttttcg atcggcaaga
     1561 catatagata tgcaacgttt ataagcatat aaacaattac tacgattaat gttccaaata
     1621 atagtcccag cggaacattt ctttgaggtt gttttatttc gcctgcgaca taggttaagt
     1681 tattccatcc atcgtaagcc caaaatgcgc ctgcaatagc tagaccgaac aaagaaataa
     1741 tatttgaagt tgatgatgcg ggcattgaaa aaccagtgga gatatttgca agactgccgg
     1801 aaccgcttgc taataataaa aatgatagaa tcactataga ggcaattttt ataaaggtaa
     1861 caaatgattg aactgctccg ccaaaaatta ctcctacata attaatcccg gttaaaaaaa
     1921 gaatacacaa aatcgctgat gctttggctc caaattctgc aaaggggaaa atgtttccaa
     1981 caattggaat a
//
LOCUS       DASWBY010000544         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_207474, whole genome shotgun sequence.
ACCESSION   DASWBY010000544 DASWBY010000000
VERSION     DASWBY010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207474"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..2645
                     /locus_tag="VF870_09785"
     CDS             342..2645
                     /locus_tag="VF870_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9252522.1"
                     /translation="MKTLLYTFSAFLLFTSIIFSQPDTLTILHVNDSHSTLEAIGPRD
                     ANLKGTLGGVSRVATLVGMTRMTEPNVMFLHAGDISIGDVFFNKYFHVPELQILSALG
                     LDAMTLGNHEFDLGPEALLGSLQYAFPVANDAFPLLSANIDFSDPTIIGMQDYVKPYV
                     IKQYGSTKVGIFGLTTPATNLTSMPSPAIVSDDIFTIAGTMVYTLRTVENCDVVILLS
                     HLGVDLDKLIASNVPGINVIVGGHDHYMYEQPITLTDPLGGTTWIVQARSNYMYAGEM
                     KLVVDNTNVQLLNYQLIPIDENIPQEPAVQAIVDGMINDIESTYGIPFFTQPFGYASS
                     FFEEEAKVLLELGAHDTPMGNLVTDAFRSLTSTDIALQAGGSIALPLWAGTFTPGDIF
                     RANGYGFNLENTLGFQLVTFDLTGMDLLTGLTFGLSEIEMNDEYLLQVSGMEYTYDGT
                     KPAEERLVSIKINGNPIDPMATYSVTTNEMLLGFMAYLGIVPANLNILTGVTEFEALS
                     EYVISQNNLIQPKSLGRVLNVGDQNARSTVIGAGWLNSSSGSYKLDKNKSDKLFFEFN
                     MIDRGLNFQPAGKVTIRYPQANLNFKSDMCDWLLLDNQTATLTGTGKINNQGNYGYML
                     VANDQPDKLRIVIWDKNDGEKIIYDNLTPQNIQGAIVMHNRIVLTKDSNEELLLAPVE
                     FSLDQNYPNPFNPSTTINYSIPEAGNVEMKVYDVLGNEVATLVNETKAPGNYSAVFDA
                     SSLASGIYIYKFSANNFLQTKKMILMK"
     gene            2716..3621
                     /locus_tag="VF870_09790"
     CDS             2716..3621
                     /locus_tag="VF870_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252523.1"
                     /translation="MKKIINIRALCCLMPLLLLCGCATVEETLYLRQAEVTGPIVCPP
                     IHLTDTTDAPSLTVSPKFTYNTKKTFTGDIEQRTSYYNIDTVFVPLENSLEWDIATIN
                     AGIDFDLALSQKFAITFGAVYSSNSTYNSWGGNIGIGFFSYKDESAFRLDAGIQINTM
                     QYDAYTVVHTKVTSIWGDVDEYTGFYHDVGNSTQVDPYFSLTYNTAYKNWPVNLFINA
                     GYSVQRLFNFEPKTNYWYWGPYTSTDLRGSCTAGFINFTPGVYFFFGESNRILLGSRF
                     FLETLVDNGNPRLFILPMLQFDFRL"
BASE COUNT         1223 a          644 c          686 g         1224 t
ORIGIN      
        1 atgtattcca aagttcattg gagatactat cccaaaggct tcaaacatcc aatcttactg
       61 ggaaaagaaa attcctttaa taaatcaatt aacccattca aaaaactgtt tttaaggctg
      121 atagaataat ttttagtctt aaatcccgca aattcatcgg atattcaaag attagattct
      181 gaactaagat gacaagagtt atgtcgcttt ggtcataatt tctaattgac aacaaataat
      241 tatcctacac atttttgaca atttcatgac aactcttttt atggttaaag ccaaattact
      301 atcgtaatta tttaagctta acaaataaaa ggagttctaa aatgaaaacc ctactttata
      361 ctttttccgc tttcctgctt tttacttcaa taattttttc acaaccagat actttgacga
      421 tccttcacgt caacgactca cattcaactc tcgaggcaat aggaccaaga gatgcaaatc
      481 ttaaaggcac tttaggcggt gtttccagag ttgccacttt agtcggaatg accagaatga
      541 cagaaccaaa tgttatgttt ctacatgccg gtgatatttc cataggtgat gttttcttta
      601 acaaatattt tcatgttccg gaacttcaaa ttctaagtgc attgggactt gatgcaatga
      661 cgcttggcaa tcatgaattt gatttgggtc ctgaagcatt acttggttct ttgcaatatg
      721 cttttccggt tgcaaatgat gcatttcccc tgctttcagc aaatatagat ttttcagatc
      781 ctacaattat tggaatgcag gattatgtta agccatatgt aatcaaacaa tatggcagca
      841 ccaaagtcgg aatattcgga ctaactactc ccgcaacaaa tttaacttca atgccttcac
      901 ctgctatagt aagtgatgat atttttacaa ttgccggaac aatggtttat actttacgta
      961 cagttgaaaa ctgcgacgtt gtaattttat tatcacatct tggagttgat cttgataaac
     1021 tgattgcttc aaatgtgcct ggaataaatg taatagttgg tggacatgat cattatatgt
     1081 acgaacaacc aataacattg acagatccgt tgggtggaac gacatggata gtccaggcaa
     1141 gatcaaatta tatgtatgcc ggcgagatga agttagttgt tgataacaca aatgttcagt
     1201 tattaaacta tcaattaatc cctattgatg aaaacattcc gcaagaacca gctgtgcagg
     1261 caattgttga tggaatgatt aacgatatag aatcaactta tggaattcct ttctttactc
     1321 aaccatttgg ctatgcaagt tctttctttg aagaggaagc aaaagttctt ttagagcttg
     1381 gagcacacga tactccaatg ggtaatttag ttactgatgc tttcagatca ttaacttcaa
     1441 cggatattgc gctgcaggcg ggcggatcaa tagcccttcc tttatgggca ggtacattta
     1501 ctccaggaga tattttcagg gccaatggct acggatttaa ccttgaaaac acgcttggat
     1561 ttcaacttgt tactttcgat ttaacgggaa tggatcttct aacaggactt acattcggac
     1621 tttccgaaat agaaatgaac gacgaatatc tgctgcaagt ctctggaatg gaatatacat
     1681 atgatggaac taaacccgca gaggagagac tagtatcaat taaaattaac ggaaatccga
     1741 ttgatccaat ggcaacttat tccgttacta ctaatgagat gttattaggt tttatggctt
     1801 atctcggaat agtacctgca aacttgaata ttcttactgg agttacagaa tttgaagctt
     1861 tatcagaata tgtaattagt cagaataatt taatccaacc taaaagcttg ggtagagttt
     1921 tgaatgttgg agatcaaaat gcaagaagca ctgttatagg tgcgggatgg ctgaactctt
     1981 catcgggttc ttataaactt gataagaata agagcgataa attatttttt gaattcaata
     2041 tgattgatcg tggattaaat tttcagcctg ccggaaaagt aactataaga tatccacagg
     2101 caaatttgaa ttttaaaagt gatatgtgtg attggctgtt attagataat caaacagcaa
     2161 cattaaccgg aacaggtaaa attaataatc agggaaatta tggctatatg ctggttgcaa
     2221 atgatcagcc ggataaatta agaattgtaa tctgggataa gaatgatggt gaaaagatca
     2281 tttatgacaa tcttacacca caaaatattc aaggtgcaat tgtaatgcat aaccgaattg
     2341 tcttaacaaa agatagtaat gaggaattat tactcgcccc tgttgaattt tcacttgacc
     2401 agaattatcc aaacccattc aacccgtcaa caacaattaa ctattcaatt ccagaagcgg
     2461 gaaatgttga gatgaaggtt tatgatgtac ttggtaatga agttgcaact ctggtaaatg
     2521 aaacaaaagc acccggtaat tattctgcag tgtttgatgc ctcatcgttg gcaagtggaa
     2581 tctatatcta caagttcagt gcgaataact ttcttcaaac caagaagatg atcctaatga
     2641 aataattatt ttttaaattt gggcatctga ttacttcatg gggggatgcc caattctaaa
     2701 aatctgaggt acgttatgaa aaagataata aatattagag cactttgctg tttaatgccg
     2761 cttttattgc tttgtggctg cgcaactgtt gaagaaacac tttacctgag gcaggctgaa
     2821 gtaactggtc caattgtatg tcccccaatc catttgacag atactactga tgcaccctca
     2881 ttaacagttt ctcccaaatt tacttataat actaaaaaaa catttaccgg tgatattgaa
     2941 caacgtacta gttattataa tatcgataca gtattcgtgc cgcttgaaaa tagtttagag
     3001 tgggatattg ccacgatcaa tgcgggaatt gattttgatt tagcgttatc tcaaaaattc
     3061 gccatcactt ttggtgctgt ttattcatca aatagcactt acaatagttg gggaggtaat
     3121 ataggtatcg gattttttag ttataaagat gaatctgctt ttagattgga tgcaggtatc
     3181 cagattaata caatgcaata tgatgcttat actgtagtgc atacaaaagt aacaagcatt
     3241 tggggcgatg ttgatgaata taccggcttt tatcatgatg tcggtaactc aacacaagta
     3301 gatccgtatt ttagcttaac atataatacg gcttataaaa actggccggt aaatttattt
     3361 ataaatgcag gatacagcgt tcaaagactt tttaactttg aaccgaaaac taattattgg
     3421 tattggggac cttatacaag cactgattta cgcggaagct gcaccgccgg atttattaat
     3481 tttactcccg gtgtttattt cttctttggc gaatcaaata ggattctatt gggctcaaga
     3541 ttttttttag aaacactagt ggataatggc aacccaagac tttttatttt accaatgctg
     3601 caatttgatt tcagacttta ggaccagatg tgtttaactt tctaattctg ttttatcttc
     3661 cgctctgaat tcatttctga attggtttgt aattaacata accatcagtc caaatattga
     3721 acctacaata atcattgagt ttccaattga taattgatta tacaaaaatt caagatt
//
LOCUS       DASWBY010000545         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_320654, whole genome shotgun sequence.
ACCESSION   DASWBY010000545 DASWBY010000000
VERSION     DASWBY010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320654"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VF870_09795"
     CDS             complement(<1..323)
                     /locus_tag="VF870_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HEX9252524.1"
                     /translation="MNIDYSHEAPTVAGRLTLAQIEELVAKPMDTKPNKKYFIALSIS
                     VSMLKIGAVFLGFSFYYGTGLWGNNQPVGWAFPIVNFVFWVGIGHAGTLISAILFLLR
                     QKWRTG"
     gene            complement(323..>1692)
                     /locus_tag="VF870_09800"
     CDS             complement(323..>1692)
                     /locus_tag="VF870_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9252525.1"
                     /translation="ETIPKQSLLANAATLLSSGAVNSKGYTLVIKENYSLADGRFAHN
                     GWLQELPHPISKITWDNYAAVSEKTCKDLGLKNNDVIEITIGDRKLSIPVFMQAGSAD
                     EVITIESGYGKNNSGTVASKVGFDAISLLSKVSNYSSWIYSGAAIKKTGDTYELASSQ
                     EHHAFDDATTKDLHKSRNIIQEATVAGYIKNPHIIKEKHHDELISVYDPHPYPGLKWG
                     MSIDLNKCTGCGDCVIACNVENNIPVVGKDQVLKSREMQWLRIDRYYSGTPDEPSVSI
                     QPMLCQHCDQAPCENVCPVVATNHSPDGLNQMVYNRCVGTRYCSNNCPFKVRRFNFFN
                     FRDHFRDSYQESPILALMHNPEVTVRSRGVMEKCTFCVQRISEARADATYDKRTLKGS
                     DVTTACQDSCGSNAIHFGDINDKESEFYKYRNHELGYYVLEELNIHPNVTYIAKLRNI
                     HSEEV"
BASE COUNT          506 a          347 c          291 g          548 t
ORIGIN      
        1 ccggtacgcc atttttgtct taataaaaac aagattgctg aaatcaaagt acccgcgtga
       61 ccaataccaa cccagaacac aaaatttaca atcgggaaag cccagccaac gggctgattg
      121 tttccccaaa gaccggtacc gtaataaaag ctgaaaccaa gaaatacagc accaattttt
      181 aacattgata cagaaattga aagagcaata aaatattttt tattcggctt tgtatccatt
      241 ggtttagcaa caagctcttc aatctgagca agtgttaacc ttccggctac agtgggcgcc
      301 tcatgggagt aatcgatatt cactatactt cctccgagtg gatgtttctg agtttagcga
      361 tgtatgtaac gtttggatga atatttaatt cttcgagtac gtaatagcca agctcgtgat
      421 ttctatattt ataaaattct gattctttat catttatgtc accaaaatgt attgcgtttg
      481 atccgcagga atcctggcaa gcagttgtta catcactgcc ttttagcgtt cgtttatcat
      541 atgttgcatc cgctcttgct tccgagattc tttgaacaca gaatgtacat ttttccatca
      601 caccacgtga tctaactgtt acttcaggat tatgcattaa agcaagtatt ggactttcct
      661 gataactatc tctaaaatga tctctgaaat tgaagaagtt aaatcttctt actttaaatg
      721 gacagttatt tgaacaatat cttgttccaa cgcagcgatt gtaaaccatc tgatttaatc
      781 catcaggact gtgattagtt gcaacaaccg gacaaacatt ttcacacggg gcctgatcgc
      841 aatgctgaca tagcataggc tgaatgctta cgctcggctc atcaggtgta cctgagtaat
      901 atctatcaat tctcagccat tgcatttccc tgctcttaag tacctgatct ttaccaacaa
      961 caggaatatt attttcaaca ttacatgcaa taacacaatc accacatcca gtgcacttat
     1021 tcaaatcgat tgacattccc cactttaaac ccgggtatgg gtgtgggtca taaacactta
     1081 tcaactcgtc gtgatgtttt tctttaatga tgtgtggatt tttaatatag ccagctacag
     1141 tggcttcttg aattatattt cttgatttgt gtaaatcctt agtagttgca tcatcaaaag
     1201 cgtgatgttc ttgtgatgaa gctaattcgt aagtgtcacc ggttttttta attgctgcac
     1261 ctgaataaat ccaactagag taatttgaaa ctttggataa caaactaata gcatcaaatc
     1321 caactttgct tgcaacagtt ccggaattat ttttaccata acctgattca attgtaatta
     1381 cttcatcagc agaaccggcc tgcataaata caggaattga gagctttcga tcaccaattg
     1441 taatttcaat aacatcatta ttctttaatc ccaagtcttt acaggttttt tccgaaacag
     1501 ctgcgtaatt gtcccacgta attttagaga taggatgagg taattcctgc aaccaaccgt
     1561 tatgtgcaaa tctaccatct gccaaagaat aattttcttt gatgacgaga gtgtaacctt
     1621 tagaatttac tgcgccgcta cttaaaagcg ttgctgcatt ggctaataag gattgctttg
     1681 ggatagtttc at
//
LOCUS       DASWBY010000546         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_736868, whole genome shotgun sequence.
ACCESSION   DASWBY010000546 DASWBY010000000
VERSION     DASWBY010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736868"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VF870_09805"
     CDS             complement(<1..537)
                     /locus_tag="VF870_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9252526.1"
                     /translation="MFEFSFTEEQNMLREMVRDFTNNEIKPIAAKIDEEARIPKELIQ
                     KMAELGFLGISFPEEYGGGGFGEVGYCLMQEEIARGCMSTATFIGAHQSIGTNAIFNG
                     ASEEQKKKFIPPLASGEKIAAFGLTEAQAGSDSFHLKTKADIVGDEWVINGEKLWITN
                     GGIADFISLFARTEKGISL"
     gene            complement(729..1091)
                     /locus_tag="VF870_09810"
     CDS             complement(729..1091)
                     /locus_tag="VF870_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX9252527.1"
                     /translation="MPVLISSPTIIEAAGNKPKIIQEYFGRVNSKTESVSIAKMTSPQ
                     GWIEPGQRPEFDEYTVVLKGTLQVKTENEIIDVKAGSTILTKKGEWIQYSTPKEGGAE
                     YVAVCLPAFSPETVRRDG"
     gene            complement(1101..1856)
                     /locus_tag="VF870_09815"
     CDS             complement(1101..1856)
                     /locus_tag="VF870_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9252528.1"
                     /translation="MQKTDYSKIANTYNKRYDENYLVNIEKEIKDIIAVNNYKNVLEA
                     GCGTGRWISSLDGDDKNIFGLDYSFDMMKIRKRDKLKFNFVNADAVQVPFKNNFFDLI
                     FCVNAIHHFPDKEKFIRECRRTLSNNGTVAVFGVDPHIDKDWYVYDYFESVYENDLKR
                     FPSLKSLMKILETENFERIETKVIEKVYHTRIGKDIFNDPFLAKHHSSQLANLSDEEY
                     QKGINKIKNQIEKNPGTAFTTSVIFYLVSAKKK"
BASE COUNT          649 a          404 c          301 g          775 t
ORIGIN      
        1 aagactaatt cctttttctg tacgtgcaaa cagtgaaata aaatctgcaa ttccgccgtt
       61 agtaatccaa agtttttcac cattaataac ccactcatca ccaacaatat ctgcttttgt
      121 ttttagatga aatgaatcag atccagcctg tgcttctgtc aatccaaatg ctgcaatctt
      181 ttcaccagag gcaagtggtg gaataaattt ctttttctgt tcttcacttg caccattaaa
      241 tatagcattg gtccctatcg attgatgagc gccaataaaa gttgctgtgg acatacaacc
      301 acgggcaatt tcttcctgca ttaaacaata accaacttca ccaaatccac cgccgccgta
      361 ttcttccgga aatgaaattc caagaaaacc aagctcagcc attttttgaa ttagttcttt
      421 agggattctg gcttcttcat caattttagc agcaataggt ttaatctcgt tgttggtaaa
      481 atcacgcacc atctcacgga gcatgttttg ttcttcggta aagctaaatt caaacataaa
      541 gaggatgcct tctttttatt gtgcgttata ttagtcaaat aatattccat aaagatttcc
      601 cttattgctt gtaaaaacaa acataaaaca agggaaattg gttcattctt ttcaaatcaa
      661 agaagagaaa gtgcagttgt tcttaattgt gagaagttag ataaaaaagc ttactttaaa
      721 actttatgct atccatctct ccttacggtt tccggagaaa aggcaggtaa gcaaaccgca
      781 acatattctg ctccaccttc ttttggagtg ctgtattgta tccactcacc tttctttgtt
      841 aagatagttg agccggcttt aacatcaatt atttcatttt cagtttttac ctgcaaagtt
      901 ccttttagta caaccgtgta ttcatcaaat tcagggcgct gcccaggttc aatccagcct
      961 tgcggacttg tcatttttgc gatgctaact gattctgttt ttgaattaac tcttccaaaa
     1021 tattcttgaa ttattttggg tttgttacct gcggcctcaa taattgttgg actgctaatt
     1081 aatactggca ttttttctcc ttattttttc tttgcgctta caagataaaa gattactgat
     1141 gttgtaaaag ctgttccagg atttttttct atttgatttt taatcttatt aattcctttt
     1201 tggtattctt catcagacag atttgctagt tgagagctat gatgctttgc aagaaacgga
     1261 tcattaaaaa tatccttgcc gattcttgtg tgataaactt tttcaattac tttagtttca
     1321 attctctcaa agttttctgt ttctagaatt ttcattaagg atttaagcga tggaaacctt
     1381 ttcagatcat tttcataaac agattcaaag taatcataaa cgtaccagtc tttatcaata
     1441 tggggatcaa cgccaaaaac agcaactgtt ccattgtttg ataatgtacg tctgcattct
     1501 ctgataaatt tttctttatc cggaaaatgg tgaattgcat taacacaaaa tattaaatca
     1561 aaaaaattat tcttaaaagg gacttgaacg gcgtccgcat ttacaaaatt aaatttcaac
     1621 ttgtcccgct tccgaatttt catcatatca aacgaataat ctaatccaaa aatattttta
     1681 tcatctccat caagcgagct tatccatctt ccggttccgc accctgcttc taaaacattt
     1741 ttatagttat ttacagcaat tatgtcttta atttcttttt caatattaac cagataattt
     1801 tcatcatacc gtttgttata tgtatttgca attttactat aatctgtttt ttgcatttta
     1861 tacgatttta taattatttc atattcaaac taaaaataac aatacgcata ctcaagaatc
     1921 cattgccaat ttgttataaa caaatattat ctttttttaa ttaaatcgtg tgcgaatcga
     1981 gcaagtaaaa catgaagaga tgcaaaaggt agattcatca tttttaacct cgaagattct
     2041 tcaaaaacct tcggagttac tttaggacta tccgaacatt ttttctaaaa cttctttacg
     2101 gtaattaaaa atctcattaa tcttttttg
//
LOCUS       DASWBY010000547         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_700466, whole genome shotgun sequence.
ACCESSION   DASWBY010000547 DASWBY010000000
VERSION     DASWBY010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700466"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VF870_09820"
     CDS             complement(<1..668)
                     /locus_tag="VF870_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX9252529.1"
                     /translation="MKSFLKIRSKNIILLMLSLIAFQSFAQKANEFRTVDNNAFREGE
                     KLTFDVKYGFVTAGIATFEIPAIKKISGRNAYHVTFEVNTVPTFDNFFKVRDRYETYL
                     DVEGIFPWRFEQHIREGKYSRDFSAFFDQRKGKAKTSEGQYDIPVYVNDIVSAFYYAR
                     TLDYSKLNVGDKIPLKNFYKDKVYDLDVVYHGKETIEVAAGTFDCLIVEPLVQEGGLF
                     KSEG"
     gene            complement(678..1709)
                     /locus_tag="VF870_09825"
     CDS             complement(678..1709)
                     /locus_tag="VF870_09825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9252530.1"
                     /translation="MITAPKNILIVRTDRIGDLILTLPLAGLIKKQYPEAKVTFLVRE
                     YTKNIVSNHPFIDEIIVLKEVDGNALLFENINLIKQKNFDSCIVVYPRFKISLIVFLS
                     RVKNRIGTGYRWYSFLFNLKVYEHRKNAERHEMEYNVNLLEKLGIKNSVAVNNVKYDL
                     RVDEHSLQNVIKILLDEKLDLQRPIIIVHPGSGGSSIDLPVNKFNELVKRLDDDNYQI
                     ILTGSKSEVELCEKIKSSAKLKNFAGKFNLDELTALISKSAMFISNSTGPIHIAAALG
                     KYTVGFYPKIVSCSQERWGPYTNKKLVYEPQLDCKNCTREQCEKLNCMDSIDISKVYS
                     DIKNVLNKF"
     gene            complement(1706..2290)
                     /locus_tag="VF870_09830"
     CDS             complement(1706..2290)
                     /locus_tag="VF870_09830"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX9252531.1"
                     /translation="MPRKFYIRPVLAVAKDLLGKIVTKKDGNRVFAGRIVEVEAYDGK
                     IDEAAHTFKGKTKRNEVMFNEGGYFYVYFTYGAHFCCNVVAGKQNHGAAVLIRAVEPV
                     LGIDKMIENRFGRELKSEKEIYNLTSGPGKVCKAFGITKEHSGLDLTNSSVFIIDQPK
                     LKINMIGVSKRIGITKSVSLQWRFFEIGNPYLSR"
     gene            complement(2360..3055)
                     /locus_tag="VF870_09835"
     CDS             complement(2360..3055)
                     /locus_tag="VF870_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252532.1"
                     /translation="MSSNSFDAFNQKVNLIYEYNNKSPLFARVAENEIEKNNIDRAIK
                     ILTEGLVNYPDFSVAYFLLSKAYTIKGNYGQALKFIKKGSELIHSPKTFDYYLREIDA
                     IKKQRQLFNVSRWADSANENFSSHAFSPGNKSENEKPTESIEQTLNKLTAEIEGATLA
                     VREAKRKLADGKPKDFANNDFIVSETLAKIYITQGEFKEAISVYKKLKIKHPDKEIYF
                     DTKIAELKSKLSA"
     gene            complement(3055..>3413)
                     /locus_tag="VF870_09840"
     CDS             complement(3055..>3413)
                     /locus_tag="VF870_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEX9252533.1"
                     /translation="RKRIVLFPLFMAGSMGLAVNNSRAVFEGLMNRKSEFVRTPKFKQ
                     ESEKDTFVGNKYLNRKLGLSVFVEAVLAVYCFIGVLSSIYFMELASIPFQVLFTLGFS
                     FIAYTSIKHAYATKKI"
BASE COUNT         1073 a          642 c          500 g         1198 t
ORIGIN      
        1 ctgccttcgc ttttgaataa gccgccttcc tgcaccaatg gttcaacaat taaacaatca
       61 aatgttcctg cggctacttc aattgtttct ttcccgtggt acaccacatc aagatcgtaa
      121 actttatctt tataaaaatt ctttaatgga atcttatcac cgacatttaa ttttgagtaa
      181 tccagtgttc ttgcataata aaaagcagaa acaatatcgt ttacataaac agggatatca
      241 tactgccctt cacttgtttt tgctttacct tttctttgat cgaagaatgc agaaaaatct
      301 cttgagtatt ttccttcacg aatatgctgt tcaaatctcc agggaaatat tccttcaaca
      361 tcaagatacg tttcatatct atcgcgtact ttaaagaagt tatcaaaagt tggaactgtg
      421 ttaacttcaa atgttacgtg gtaagcattt ctaccggata tttttttaat cgctggtatc
      481 tcaaaagttg caattccggc agtcacaaaa ccgtatttta catcaaaggt aagcttttct
      541 ccttcgcgga atgcgttatt atcaaccgtt ctaaattcat ttgctttttg cgcgaatgat
      601 tgaaaagcga taagtgaaag cattagcaaa atgatatttt ttgatcttat ttttaaaaag
      661 gatttcattt acttctccta aaatttattt agaacatttt taatatcgct gtacacctta
      721 cttatatcaa ttgaatccat acaattcaat ttttcgcatt gttctcgtgt acaattttta
      781 caatctaact gaggctcata aacaagtttt ttatttgtgt atggtcccca tcgttcctgc
      841 gagcaggaaa caatttttgg ataaaacccg acagtatatt taccaagtgc cgctgcaatg
      901 tgtattggtc cagttgagtt agaaataaac attgctgatt tactaatcaa ggcagttaat
      961 tcatcaagat taaatttgcc ggcaaaattt ttcagtttgg ccgatgattt aattttctca
     1021 caaagttcaa cttcactttt actcccggtt aaaataattt gataattatc atcatcaagt
     1081 ctttttacga gctcattaaa tttatttacc ggtaaatcga ttgagctgcc gccgctgccc
     1141 ggatgcacta tgataatggg tctctgtaaa tctaattttt catctaaaag aatcttgata
     1201 acattttgca gagaatgttc atcaactctt aaatcgtatt taacattatt aactgcaaca
     1261 ctgtttttaa ttcccagttt ttcgagaaga ttaacattgt attccatctc gtgccgctca
     1321 gcattttttc tgtgctcata aacttttaga ttaaacaaaa acgaatacca gcgatacccg
     1381 gtcccaattc tgtttttaac tctgcttaaa aaaactatca agcttatttt aaatcttgga
     1441 taaacaacaa tacagctatc aaaatttttc tgtttaataa gatttatatt ttcaaacagg
     1501 agtgcatttc catcaacttc tttaagaaca attatttcat caataaatgg atgattgctt
     1561 acaatatttt ttgtgtactc acgaacgaga aaagttactt tagcttcagg atattgtttt
     1621 ttaatcagtc cggctaaagg taaagttaaa ataagatcac caatacggtc agttctaacg
     1681 atcaaaatat ttttgggtgc tgtaatcatc ttgataaata tggatttccg atttcaaaaa
     1741 atctccactg taaactaact gattttgtaa ttccaatcct tttagaaaca ccaatcatat
     1801 taatcttaag cttcggctga tcaataataa ataccgagct gtttgttaaa tctaaacccg
     1861 aatgttcttt agtaatccca aaagctttgc aaacttttcc gggtccgctt gtaaggttat
     1921 aaatttcttt ttctgatttc aattctctgc caaatctgtt ttctatcatt ttatcaattc
     1981 cgagtaccgg ttcaaccgca cgaattaaga ctgcggctcc atgattttgt tttccagcaa
     2041 caacattaca gcaaaaatga gcaccataag taaagtaaac ataaaaatat ccgccttcat
     2101 taaacatcac ctcgttacgt tttgtttttc ctttgaatgt atgcgcagct tcatcaattt
     2161 ttccatcata agcttctacc tcaacaattc gcccggcaaa aacacgattc ccatctttct
     2221 tagtgactat cttaccaagc aagtcctttg ccacagcaag aacagggcga atatagaatt
     2281 tccgaggcaa aatcttgttt tgggttgtat caatcatagt acaaaggtaa taaaaaagta
     2341 tgcaggataa ggagaatatt taagcactta acttggattt taattcagca attttagtat
     2401 caaaataaat ttccttatca ggatgtttta tttttagctt tttataaaca gaaattgctt
     2461 ccttaaactc accttgagtt atataaattt tagcaagagt ttcagaaaca ataaaatcgt
     2521 tatttgcaaa atcttttggt ttcccgtccg ctaattttct tttagcttct ctaactgcca
     2581 gggtggcgcc ttcaatttcg gctgtaagtt tattgagtgt ttgttctata ctttcagtgg
     2641 gtttttcatt ttctgatttg tttccaggag aaaaagcatg acttgaaaag ttttcattcg
     2701 cagaatctgc ccagcgagaa acattaaata gctgtctttg ttttttaatt gcgtcaattt
     2761 cacgaaggta ataatcaaat gttttaggag aatgaataag ttcgcttccc ttttttatga
     2821 actttaacgc ctgtccgtag ttacccttta ttgtataggc tttacttaga agaaaatatg
     2881 caacagaaaa atcagggtaa ttaactaatc cctcagttaa aattttaatt gcccgatcaa
     2941 tattattttt ttcaatttca ttttcagcaa cacgggcgaa caatggcgat ttattattat
     3001 actcgtaaat taaatttact ttttgattga atgcatcgaa tgaattgctg ctcattaaat
     3061 tttctttgtt gcgtaagcgt gtttaattga tgtataagct ataaatgaaa atcccaaagt
     3121 aaataatacc tggaatggga ttgaagctaa ttccataaaa tagattgaag acaacactcc
     3181 gataaaacaa taaaccgcaa gaacagcttc aacaaaaaca gaaagtccaa gttttctatt
     3241 taaatatttg tttccaacaa aggtgtcctt ttcactttcc tgtttaaact ttggagtgcg
     3301 cacaaattcg ctcttcctat tcattaaacc ttcaaaaaca gcacgagagt tattgactgc
     3361 caggcccata ctgcctgcca taaacagcgg aaaaagcaca attcgttttc gcc
//
LOCUS       DASWBY010000548         4085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_25239, whole genome shotgun sequence.
ACCESSION   DASWBY010000548 DASWBY010000000
VERSION     DASWBY010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25239"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VF870_09845"
     CDS             <1..564
                     /locus_tag="VF870_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX9252534.1"
                     /translation="STSSSTTSPKGDVKSIDSEELAMLLDSVSSVIIVPGYGMAVGQA
                     QHAVRDLMNVLEKKGIKVRFAIHPVAGRMPGHMNVLLAEAQVPYDKLLAMEDINDDFN
                     NTDVVIVIGANDVVNPAARHDQSSPIFGMPILNVDYAKTVIINKRSMSAGYAGIDNEL
                     FFYPNALMYFGDSKEAMTKLVNEIKNL"
     gene            785..2005
                     /locus_tag="VF870_09850"
     CDS             785..2005
                     /locus_tag="VF870_09850"
                     /inference="COORDINATES: protein motif:HMM:NF028426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system plug protein
                     domain-containing protein"
                     /protein_id="HEX9252535.1"
                     /translation="MKKLFVFLFIIQASIILPQEVVIKSLKTYTTKDANSIPVLSSPR
                     DLLTIDFDVQAEYPPNFNVIFKFCDRNWNPTDNIFLLNQGQNTAYTLSYFSLPTTVED
                     AKYHYTNTFPDKDGYVSFPFSGKWKFYIVDSQDPSLIFAEGKFFAIKTDVPLKVDIKR
                     ETLEDKTYFPPQLGYSNWIIVDATPKDDYFPFFVDELEIVQNHLFNYSVKVLRNSTDQ
                     NRVFEWDGGSKIRFTAKDVFPGNEYRQTNLMDINIYNSKNVKAQFDGMEYSRFLLMSK
                     RDNNGSFTLAPPKDIYSTYMNVTFQIKPPEEVYGDIYLVGAFNDWEVSEKYKMNFNGD
                     HFELTILLKRGAYDYQYVVVNGDYSDVTNQNWFILEGNDWQTTNEYNIFLWYRDQEYG
                     GYDRVIGYSKIQTR"
     gene            2011..3000
                     /locus_tag="VF870_09855"
     CDS             2011..3000
                     /locus_tag="VF870_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9252536.1"
                     /translation="MEKLSVGVIGVGHLGKLHSKMFSQIDNCEFVGVFDSNPEQAKLV
                     ADEFGVKQFDSIDELLSKVKAVSIAATTSAHYDLAKKCFEKNIHVFVEKPITATIEQG
                     EELVKIAKQKNLKFQVGHIERFNPGLLSLETYISDPMFIQSDRLAQFNPRGTDVAVVL
                     DLMIHDIDIILSFIKSDVKQVDASGVAVVSNHIDIANARIQFENGAVANVTASRISQK
                     KMRKMRIFQKDHYISLDFVTGVSEVYRIQPVDEAALPNSISFGEIGVGDKRKRLIYEQ
                     PESKDINALNYELQLFVDSVLYDKKIVVSGEDGLRALKVAEIIIQKIEESKQS"
     gene            3002..3613
                     /locus_tag="VF870_09860"
     CDS             3002..3613
                     /locus_tag="VF870_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252537.1"
                     /translation="MKKLFFITVIFAFILSGIRCSVYESMTNLSRLQFKVGNVNGFQI
                     NGVSISNKSKLNDFNPLDIINISAAFAKGTLPASFVLNVDAKNPNDGTGGYPKSDATL
                     QGFKWRLFLDDKETISGDIDNPVTIPGTGDVTTIPLRINIDLMKFFNDKGYESLINLA
                     LALGGTQGSSSKVTLYATPTVSSPFGNITYPGELKLVDTQFSK"
     gene            3636..>4085
                     /locus_tag="VF870_09865"
     CDS             3636..>4085
                     /locus_tag="VF870_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9252538.1"
                     /translation="MAKTEKYALGVDLGGTNIKIGIVSDKGKLLKTISVKTEADGGPK
                     KVIANIIKGIEIILTKNKYKIQGIGIGCPGVVSIKKGIVENAPNLPGWKNVKLGPIIK
                     EKFGYKVHLENDANAAAIGELIFGAGKKMDSFVMVTLGTGVGGGIVFN"
BASE COUNT         1388 a          625 c          769 g         1303 t
ORIGIN      
        1 agcacaagtt cttcaacaac ctcacccaaa ggagatgtta aatctattga ttcagaagaa
       61 ctggcaatgc tgcttgattc agttagcagt gtaattattg ttcccggata cggaatggca
      121 gttgggcagg ctcagcacgc tgtacgtgat cttatgaacg ttctggagaa aaaagggatt
      181 aaagttagat ttgcgattca tccggttgct ggtagaatgc caggacatat gaacgtattg
      241 cttgcagaag cacaagtacc ttatgataaa cttttagcaa tggaagatat taacgatgat
      301 ttcaacaaca cagatgttgt aattgtaatt ggagcaaatg atgttgtaaa tcctgcagct
      361 cgtcacgatc aaagtagtcc gatttttgga atgccgattc ttaatgttga ttatgcaaaa
      421 acagttatca taaataaacg ttcaatgagt gctggatatg caggcatcga taatgaatta
      481 ttcttctatc caaatgcgct gatgtatttt ggagattcta aagaagcgat gaccaagttg
      541 gttaatgaaa taaagaatct ttaatgattt gaatcttttt ttaaacccag ttcctcccca
      601 gggactgggt ttcgtgttta taccctaaac cacgtcactt aactcgtgat ttcactattc
      661 tccaattcca acattccggt atcacttggt tggggatttt tatttccaca ctgaacactg
      721 tttaacatca attttaaggc atatatttgt aatgttgttt ttattcaaaa aatcaggaaa
      781 aattttgaaa aaactatttg ttttcttgtt cataattcag gcttctataa tccttcctca
      841 ggaagtagta attaaaagtc ttaaaacata tactactaag gatgctaatt caattccggt
      901 tttatcttca ccacgggatt tgttaacaat tgattttgat gttcaagctg aatatcctcc
      961 taactttaat gttatcttta agttttgtga taggaactgg aatccaacgg acaatatttt
     1021 tttgctgaat cagggacaaa atactgctta cacacttagt tacttttcac ttccaacaac
     1081 ggttgaagat gcaaaatatc attacaccaa tacttttcca gataaggatg gatatgtttc
     1141 attccctttt tcaggtaagt ggaagtttta cattgttgat tcacaagatc catctttaat
     1201 ctttgcagaa ggaaaatttt ttgcgattaa gacagatgtg ccgttaaaag ttgatatcaa
     1261 aagagaaact ttagaagata agacttattt ccctccacaa cttgggtaca gtaattggat
     1321 aattgtagat gcaacaccca aagatgacta ctttccattc tttgttgatg agcttgagat
     1381 agttcaaaat catttgttta attattctgt taaagtgctc cgaaattcta cagatcaaaa
     1441 cagagttttt gaatgggacg gcggctctaa aataagattt acagcaaaag atgttttccc
     1501 cggaaatgaa taccggcaaa caaatttaat ggacataaat atctataact ctaaaaacgt
     1561 taaagctcag tttgatggaa tggaatacag cagatttttg ctaatgagca aaagagataa
     1621 taacggaagt ttcactttag cacctccaaa agatatttac tcaacttata tgaacgtgac
     1681 gtttcagatt aaaccgcctg aagaagttta cggagatatt tatttagttg gtgcttttaa
     1741 tgattgggaa gtctcggaaa aatataaaat gaatttcaac ggcgatcatt ttgaattaac
     1801 tatcctgctt aaaagaggag cttatgatta ccaatatgtt gttgtaaatg gtgattattc
     1861 tgatgtcaca aatcagaatt ggtttatact tgagggaaac gattggcaaa ctacaaatga
     1921 atataatatt tttctatggt atcgtgacca ggaatatggt ggttacgatc gtgtaattgg
     1981 ttattcaaag attcaaacga gataaaaatt atggaaaaac tttcagttgg tgttattggt
     2041 gtcgggcatc tcggcaaact tcattcaaaa atgtttagtc aaattgataa ctgtgaattt
     2101 gttggtgtgt ttgattcaaa tccagagcag gcaaaattgg ttgctgatga atttggtgtt
     2161 aaacaattcg attcaatcga tgaattactt tcaaaagtaa aagcagtttc aattgctgca
     2221 acgacaagtg ctcattacga tcttgcaaaa aaatgttttg aaaaaaatat tcatgtattt
     2281 gtggaaaaac caattactgc aactattgag cagggtgaag aattagtaaa gattgcaaag
     2341 caaaagaatt taaaatttca agtcgggcat attgaacgat ttaatccggg attgctttca
     2401 ttagaaacgt atatttccga tccgatgttt attcagtcag acaggctggc acaatttaat
     2461 ccacgcggaa cagatgtcgc ggttgtatta gatttaatga ttcatgatat agatattatt
     2521 ttaagcttta taaaaagcga tgttaaacag gttgatgcaa gcggcgtagc agttgtttca
     2581 aatcatatcg atattgcaaa cgctagaatc cagtttgaaa acggtgctgt agcaaatgta
     2641 actgcaagca gaatatctca aaagaaaatg cgtaagatgc gtatcttcca aaaagatcat
     2701 tatatttctt tagattttgt aacaggagtt tcagaagttt atcgtatcca gcctgttgat
     2761 gaagcagcat tgcctaactc aatttcattc ggtgaaattg gtgtgggaga taaaagaaaa
     2821 agattaattt atgaacaacc cgagtctaaa gatataaatg cactaaatta tgaattgcag
     2881 ctttttgtag attctgtttt gtatgataaa aaaattgtag tttcaggcga agatggtctt
     2941 cgcgcactta aagttgcaga aattataatt caaaaaattg aagaatcaaa acaaagctaa
     3001 aatgaaaaag ttatttttta taacagttat atttgctttt attctgtcag gaataagatg
     3061 cagcgtatat gaatccatga caaacctttc cagacttcaa tttaaagtcg gaaatgttaa
     3121 tggttttcag ataaatggag tttccatttc aaacaaatct aaactgaacg atttcaatcc
     3181 actggatatt ataaatatat ccgcagcgtt tgcaaaagga acattaccag cttcatttgt
     3241 acttaatgtt gatgctaaaa atcctaatga tgggacaggc ggttacccca aatccgatgc
     3301 aacattgcaa ggatttaaat ggagattatt tcttgatgat aaagaaacaa tttcaggaga
     3361 tattgataat ccggttacaa ttcccgggac aggtgatgta acaactattc cacttagaat
     3421 taatattgat ctgatgaaat tctttaacga taaaggatat gaaagtttaa ttaatctcgc
     3481 attagctctt ggcggtaccc aaggatcatc gtcaaaagta actttatatg caacgccaac
     3541 tgtcagttct ccctttggaa atataactta tcctggtgaa ttaaaattag tcgatactca
     3601 gttttcaaaa taaaattatt ggtcaggatt ttattatggc aaaaacagaa aaatatgctc
     3661 tcggtgttga tttaggcgga acgaatatca aaatcggtat tgtttccgat aaaggtaagc
     3721 ttcttaaaac catttctgta aaaaccgaag ctgatggtgg acccaaaaaa gttattgcca
     3781 atatcatcaa aggcattgaa ataatcctga caaaaaataa atataaaatt cagggaattg
     3841 gaattggttg tccgggtgta gtgtcaataa aaaaaggaat tgttgaaaat gcaccaaatc
     3901 ttcccggatg gaaaaatgta aaacttggcc ccatcattaa agagaaattt ggctataaag
     3961 ttcatcttga aaatgatgca aacgctgctg caattggtga attaattttt ggggctggaa
     4021 aaaaaatgga ttcttttgtt atggtaactc tgggcacagg tgtaggcggc ggaattgttt
     4081 ttaac
//
LOCUS       DASWBY010000549         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_172125, whole genome shotgun sequence.
ACCESSION   DASWBY010000549 DASWBY010000000
VERSION     DASWBY010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172125"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VF870_09870"
     CDS             complement(<1..783)
                     /locus_tag="VF870_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX9252539.1"
                     /translation="MKEKIRIASGQGFWGDLIDAPYYQVTKGDIDYLVMDYLAEVTMS
                     ILQKQKNRNPELGYAKDIPELMQRILPIISAKKIKVITNGGGVNPVACAKAVMKVAKE
                     LEIKNLKIGVVLGDDIKDQIDDLIKSGAPLKNMETDEPVSIVQDKILSANVYFGAFPI
                     VEALQKGADIIITGRTTDTGLTLAPMIYEFGWKNTDYDLLSAGTVAGHILECGGQSSG
                     GNFLGDWQSIPNLAEIGFPIAEAYPNGEVIITKHPNTGGRVSF"
     gene            complement(767..1504)
                     /gene="miaA"
                     /locus_tag="VF870_09875"
     CDS             complement(767..1504)
                     /gene="miaA"
                     /locus_tag="VF870_09875"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX9252540.1"
                     /translation="MEPDISFNASMFADRAEVILNDLIKQGRIPIVVGGSGLYVKALI
                     DGISESADTNNELRVELLDLRQKYGNEFLYNELKKVDEISASKMLPQNWKRVMRALEV
                     FRLTGKPIWQHHSNQIRTSRFQYSQIGLLWDRRILYENIEERVDSMINSGLVDEVKFV
                     LDSGYDKNINSLNTVGYKEIIQYLENEISLDRAVELIKRNTRRYAKRQMTWFNADKRI
                     EWVHINSKTDLDLLADKIAKEINERKN"
     gene            complement(1509..1697)
                     /locus_tag="VF870_09880"
     CDS             complement(1509..1697)
                     /locus_tag="VF870_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEX9252541.1"
                     /translation="MEEVIVIVGPTCSGKTYLSLKLTSMLSTEIISADSRQIYKLLNI
                     GTAKPSIGELKKNKTPFY"
     gene            complement(1685..2323)
                     /locus_tag="VF870_09885"
     CDS             complement(1685..2323)
                     /locus_tag="VF870_09885"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX9252542.1"
                     /translation="MFRVQREELVDEITRKGITDLAVIKAIGIVPREKFVEKTMHHMA
                     YRDVALPIGYDQTISQPYTVAFMTQLLNIKKPGMKILEIGTGSGYQAAVLSEMKADVY
                     SIERNIDLHHRATKLFDKLGIRVHAKYGDGTIGWKEFSPFDGIIVTAGSPVIPQKLKD
                     QLAIGGRMVIPVGDRASQKLYLLKKISETEFDSEIIPEFSFVPLIGREGWKK"
     gene            2410..2928
                     /locus_tag="VF870_09890"
     CDS             2410..2928
                     /locus_tag="VF870_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HEX9252543.1"
                     /translation="MFEAISGAVLIMFMRICDVSIGTMRTILVVQGRKYLAGLAGSIE
                     VLIWVFAIRYIFQNLDQVINLFGYSIGFGLGNIIGITIEQKIGLGFAQLNIISKIASE
                     KIIEILRKQKYGVTVLPAHGVSGQVSIIVVIIPRKFQKKIIKSIESIDPNAFITVQHS
                     LPYRGFIHGSRK"
     gene            3066..>3927
                     /locus_tag="VF870_09895"
     CDS             3066..>3927
                     /locus_tag="VF870_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252544.1"
                     /translation="MRKLCTFLFVLVVTIAFISTGFSQNFSAAQNQKPGGSPNGTESL
                     VTINYDGDNFTSIGDGGTTFIAGARFTPSITGPLTGGELRYVQFYYAQLATGLTIKIY
                     DSGTASVPGALLLNQPLNLGTLIVPGWNEVELSTYIPISGNDLWICLEVADASATNFP
                     LGCDAGPANANGDYVNDTGVWQHLVDFGLNYNWNIRGVVEDTPAPLPLFFENFDSYTA
                     GNLVACSNPTDWTTWSNAPCGAEDALVSSDFAHSGTNSAKIITNDDLVKKYGATAFTS
                     GKYKISFWAYI"
BASE COUNT         1178 a          759 c          622 g         1368 t
ORIGIN      
        1 aaatgaaact ctgccaccag tgtttggatg ttttgtaata ataacttctc cattcggata
       61 agcctcagca atgggaaatc ctatctcggc caaatttggg attgactgcc aatcacctaa
      121 aaaatttcca ccacttgatt gacccccgca ctccagaata tgtccggcaa ctgttccggc
      181 agataaaaga tcataatcag tattcttcca accaaattca taaatcatcg gagcaagggt
      241 tagaccagta tcggttgttc ttccggtaat aataatatct gccccttttt gaagagcttc
      301 aactatgggg aaagcaccaa agtaaacatt cgcactaagt attttatcct gaacaattga
      361 aaccggttcg tcagtctcca tattttttaa aggagcgccg ctttttatca aatcatcaat
      421 ctgatccttg atatcatcac ctaggactac tcctattttt agattcttga tttcgagttc
      481 tttagctact ttcattacag ccttggcaca ggcaacagga ttgacaccac cgccatttgt
      541 aattactttt attttcttcg ctgagatgat tggtaatatc ctttgcatca actcaggaat
      601 atctttggca tagccaagtt caggatttct atttttctgt ttttgcaaaa tagacatagt
      661 aacttcagca agataatcca ttacaagata gtctatatct cctttagtta cttgataata
      721 gggcgcatca attaaatccc cccaaaaccc ttgtccggat gcaattctaa ttttttcttt
      781 catttatttc ctttgcaatt ttgtcagcga gtaaatctaa atcagtttta ctgtttatat
      841 gaacccattc aattctctta tccgcattaa accaggtcat ctgtctttta gcatatcttc
      901 gcgtatttct tttaattaat tcaacagcgc gatccaatga aatttcattt tctaaatatt
      961 gaattatttc tttatatcca actgtattta atgaatttat atttttatca tacccagagt
     1021 cgagaacaaa tttaacttca tcaactaaac cagagttaat cattgaatca actctttctt
     1081 caatattttc ataaagaatt ctcctatccc acaaaagacc gatttgacta tactgaaaac
     1141 gcgaagtacg tatttgattg ctgtggtgtt gccagattgg ttttccagtc aacctaaata
     1201 cttccaatgc tctcatcaca cgcttccagt tttgcggcag cattttactt gcacttattt
     1261 catcaacctt tttcaactcg ttatatagaa attcattacc atatttttgt ctgagatcca
     1321 gtagttctac tcgtaattca ttattcgtgt cggctgattc actaattcca tcaattaatg
     1381 cttttacata taagccagat ccaccaacta caataggaat tctaccctgt ttaatcaaat
     1441 cattcagaat aacttccgct cgatccgcga acatactagc gttaaaactg atgtcaggtt
     1501 ccatttcatc aataaaatgg tgttttattt ttttttaatt caccaatcga tggttttgct
     1561 gttccaatat ttaaaagttt gtaaatctgt ctgctatcag cagagattat ttcagtactt
     1621 agcatagagg ttaattttaa acttaaataa gtttttcccg aacaagttgg accaacaatt
     1681 acaattactt cttccaacct tctctgccta ttaatggaac aaaagaaaac tctggaatta
     1741 tttcagaatc aaattcagtt tccgaaatct tttttaacaa atataatttc tgggacgctc
     1801 tatcaccaac aggtattacc attctgccgc caatagccaa ttgatctttt aatttttggg
     1861 gtataaccgg ggaaccagcc gtaacaatga ttccatcaaa aggagaaaat tctttccagc
     1921 ctattgtccc atcaccatat tttgcatgta ctctaattcc aagcttatca aaaagttttg
     1981 tggcacgatg gtgaagatca atatttcttt cgatgctgta aacatcagct ttcatttcag
     2041 acaatactgc tgcctgataa cctgaacctg tgccaatttc taagatcttc attcctggtt
     2101 ttttgatatt caataactga gtcataaaag ctaccgtata tggctgagat attgtttgat
     2161 catatccaat tggcaatgca acatctctat atgccatatg gtgcattgtt ttttcaacga
     2221 acttttctct tggtacaata cctatagctt taattaccgc aaggtctgta ataccctttc
     2281 tggttatttc atcaactaat tcttctcttt gtactctaaa catttatcca tattattttt
     2341 aatttgttca gtaaagataa aattttttgt gtaagtattt ataattaaaa aatgaaagtt
     2401 tgataaaaca tgtttgaagc aatttcaggt gcggtattaa taatgtttat gcgtatttgc
     2461 gatgtatcaa tcgggacaat gagaacaata ttagttgtac agggaagaaa gtatcttgca
     2521 gggctagcgg gttctattga agtacttatc tgggtttttg cgattagata catttttcag
     2581 aatcttgatc aagtaataaa tctgtttggg tactcaatcg gctttggtct tggtaacatt
     2641 attggtatca caatcgagca aaaaattgga ttaggttttg ctcaacttaa tataatctca
     2701 aaaatagcga gtgaaaagat tattgagata ctaagaaaac aaaagtacgg tgtaactgtt
     2761 ctgcctgcac acggagtttc gggacaggta tcaatcatcg tcgttattat cccaagaaaa
     2821 tttcagaaga aaataattaa atcaattgaa tcaattgatc ccaatgcctt tattactgtg
     2881 cagcactctt taccataccg tggatttatt catggttcaa gaaaataatt caatctaaag
     2941 ttgaagttta tttttaaatt agttaatatc aatacaagat ttctttaaat gttataatat
     3001 caggattcag tagttatttt atttacttaa ttcaaattaa tttccaacca aattaaggag
     3061 tgatcatgcg taaattatgt acttttctat ttgttcttgt tgttactatt gcattcatat
     3121 cgactggttt ctcgcagaac ttttcggctg ctcaaaacca aaaaccaggt ggttcaccaa
     3181 atggcaccga atcattggtt accattaatt acgacggtga taattttaca tctattggtg
     3241 atggcggaac cacatttatt gccggtgctc gttttacccc aagcattaca ggtcctctta
     3301 ccggcggtga gcttcgctac gttcagtttt attatgcaca attagcaact ggtctaacaa
     3361 taaaaattta tgattcaggt acagcatctg ttcctggagc attattatta aatcagcctt
     3421 taaaccttgg aacattaatc gtcccaggtt ggaatgaagt tgaattgtca acatatattc
     3481 ctatttccgg aaatgatctt tggatatgtt tagaggttgc tgatgcatca gcaacaaatt
     3541 ttccgttagg ctgtgatgca ggcccagcaa atgcaaatgg tgactatgtt aatgatacag
     3601 gcgtttggca gcatcttgta gattttggat taaattataa ctggaacatt cgtggtgtgg
     3661 ttgaagatac acctgcccct ctgcctcttt tcttcgaaaa ttttgattcc tatactgcag
     3721 gcaatttagt agcttgttca aatcctaccg attggacaac ctggagcaat gctccttgcg
     3781 gtgctgaaga tgcattagtt tccagcgatt ttgctcattc cggaactaat tctgctaaaa
     3841 ttataacaaa cgacgattta gtaaagaaat atggtgctac tgcttttaca tccggtaaat
     3901 acaaaatcag tttctgggca tatattc
//
LOCUS       DASWBY010000550         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_736981, whole genome shotgun sequence.
ACCESSION   DASWBY010000550 DASWBY010000000
VERSION     DASWBY010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736981"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VF870_09900"
     CDS             <1..688
                     /locus_tag="VF870_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252545.1"
                     /translation="DKEQVYSQVERNNLLVSVKRSLRDNSRFLEMTSLKAADLEAVKE
                     QEQNFVTMWRKIGPKLVDVYANKKDKSAELKEIDNLFNVWANRINKEAWESINEEFSL
                     NNINLQNFNNGKEFVNVITQYISDEIKNYGVKDKSDSERAYTVFTDSVWFKNISNEWM
                     PYLLDNNLLTVEQKDTIEKKLAEWKSIVSPQDYTWLYAVAGLGLLFVIVLIFILKKKK
                     TPTNTAPIQS"
     gene            complement(774..1544)
                     /locus_tag="VF870_09905"
     CDS             complement(774..1544)
                     /locus_tag="VF870_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9252546.1"
                     /translation="MGKKIAIAVPKGGVGKTTTAVNLAAAFAIAEKKTLLIDFDPSGA
                     CSVSLGFENEVLRGDIFQVVSFVKHIDSVIHKTELPTLDFIPCSINSGEIEERINRLT
                     RNIYLFENILNQYALEQYDYIIIDCPPYLKGLTTVALTAADSVLVPIRAGQFSISALK
                     KMYNHILWVKENLNSKLFVEGILLTMFEPNTKAWGLTKQALFENFGSDMLQTVIPKST
                     TITEAEFYGKPTMLFNVKSSGSVAYLQLANELMNKSLV"
BASE COUNT          648 a          281 c          327 g          599 t
ORIGIN      
        1 agataaggaa caagtgtact ctcaggttga aagaaataat ctacttgttt cagtaaaaag
       61 atctttgagg gataacagcc gatttcttga aatgacatca cttaaagctg ctgatcttga
      121 agctgtaaag gaacaagagc aaaactttgt tactatgtgg cgcaagatcg gaccgaagct
      181 tgtagatgtg tatgcaaata aaaaagataa atcggcagaa ttaaaagaaa ttgataatct
      241 gtttaatgtt tgggctaata gaattaataa agaagcatgg gaatcaataa atgaagagtt
      301 ctcattaaac aatatcaatc ttcagaattt taacaacggg aaagaatttg ttaatgttat
      361 tacccaatac atttcagatg aaataaaaaa ctacggtgtt aaggacaaaa gtgattccga
      421 aagagcgtac actgttttta ctgatagcgt atggttcaaa aatatttcca acgaatggat
      481 gccatattta ctagataata atttgcttac agttgaacag aaagatacca tagaaaagaa
      541 actagcagaa tggaaatcaa ttgtatcgcc gcaggattac acctggcttt atgcagttgc
      601 aggattaggt ttactttttg ttattgtatt aatctttata cttaaaaaga aaaagactcc
      661 gacaaataca gcgcccatac aaagttaaag attaaaatat ttataattaa ttattaaagc
      721 cctgattttt tcagggcttt tttgtatcac cattattgta aaaatgacta cacttacacc
      781 aaacttttat tcattaattc atttgctaac tgtaagtagg caacggagcc gcttgattta
      841 acattaaaca acattgttgg tttgccgtaa aattcagctt cagtaatcgt agtgcttttg
      901 ggaatgaccg tctgaagcat atcactgcca aaattttcaa acagtgcttg tttagttaag
      961 ccccatgctt tggtgtttgg ttcaaacatg gtaagcaaaa ttccttcaac aaataatttt
     1021 gagtttagat tttctttgac ccataaaata tgattgtaca ttttcttaag agcagatata
     1081 gaaaattgcc cggcacgaat tggcaccaaa actgaatctg ctgctgttaa tgcaacagtt
     1141 gttaacccct ttagataagg cgggcaatca ataataatat aatcatattg ctcaagtgca
     1201 tattgattga gtatgttttc aaataaatag atattccttg ttaatctgtt tattcgttct
     1261 tcaatttctc cggagtttat gctgcatgga atgaaatcta atgtcggtaa ttcggtttta
     1321 tgaataacgg aatcgatgtg tttaacaaaa cttacaacct gaaaaatatc accgcgcagc
     1381 acttcatttt caaaaccgag cgaaacagag catgcacccg aaggatcaaa atcaattagc
     1441 agggttttct tttctgcaat cgcaaatgca gccgcaagat ttacagcggt tgtagttttt
     1501 ccaactccgc ctttaggaac agcaattgcg attttcttgc ccacaagtgc ctttatcgtt
     1561 ataagaaaat gatttaatta gtatacaatt gtgaatctat aaatttgttt ttgctttaac
     1621 aatatggatt tgattgattt gattgtgata actatcataa aatcgggcta gaagaagaaa
     1681 atgaatttat attattgctt tattgttata agtttaaatt aaagtttcat caaggacaat
     1741 tttattaaaa ttaaaatttt taggaggcaa tatgaaagtt ggaatattag gttccggagt
     1801 agttggacaa actcttgcaa ccggaattat taaatatggt catcaggtaa aaatt
//
LOCUS       DASWBY010000551         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_172304, whole genome shotgun sequence.
ACCESSION   DASWBY010000551 DASWBY010000000
VERSION     DASWBY010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172304"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF870_09910"
     CDS             <1..213
                     /locus_tag="VF870_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252547.1"
                     /translation="IPNCAASIAIAQAFLHTGLSLGATIAGLSSGAGFGPIVLFKDGE
                     INTSIKILFLTLISAISWGFIIHFIF"
     gene            237..1016
                     /gene="xth"
                     /locus_tag="VF870_09915"
     CDS             237..1016
                     /gene="xth"
                     /locus_tag="VF870_09915"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX9252548.1"
                     /translation="MKKMRLLSWNVNGIRAIQKKGFVDWLLTEDPDILCLQETKAAPE
                     QLSDELLNINGYKSYFSSSIVKKGYSGVAIYSKHEPLKVDNGFGIPKFDDEGRIIIAD
                     YKDFVLLNIYYPNGKKNEERLQYKMDFYDAFLDYTNKLVKKGKNLVICGDVNTAHKEI
                     DLARPKENSKVSGFLPIEREWLDKFFSHNYHDTFRLFNDEPENYTWWDQVTRARDRNV
                     GWRIDYFFVSDNFKKNVKDAFIMPDVMGSDHCPVGVDIEIK"
     gene            1086..1400
                     /locus_tag="VF870_09920"
     CDS             1086..1400
                     /locus_tag="VF870_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252549.1"
                     /translation="MKHIYTTISLYFMFAVIVFLQIVTPAQQKSLAEAKLVVDSFSPQ
                     KDSSTDEILNYGYGHKVLFISEIFIPNDSPRKEISISTKLTSLICISKQSSTPYGCSV
                     KY"
     gene            1442..>2229
                     /locus_tag="VF870_09925"
     CDS             1442..>2229
                     /locus_tag="VF870_09925"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX9252550.1"
                     /translation="MINEKRVRLLQKGNETKGPIVYWMSRDQRVHDNWALLFAQKLAL
                     EKQRSLAVVFSLVPDFLESTIRQYGFMLKGLQEAESELSKHNIPFFLTSGEPEVEIPE
                     FIKNINASIIVSDFDPLKIKRIWKRDVAKQISIPFYEVDAHNIVPCLHVSDKLEFAAY
                     TIRPKIHKALIEFMDEFPSLKKMRKSEISSDKIDWTKIQKSLKINFEVKEVEWIKPGE
                     VEASKTLEHFLKNKFENYHELRNDPTKEYQSNLSPYLHFGQISA"
BASE COUNT          791 a          333 c          381 g          724 t
ORIGIN      
        1 atacccaatt gtgcagcatc aattgcaata gcacaagctt ttcttcatac gggattatca
       61 ctcggagcaa caattgcagg gctaagttca ggtgcaggtt ttggaccaat agttttattt
      121 aaggatggag aaattaatac atccataaaa attcttttct taactttgat cagtgctata
      181 agttggggat ttataattca ttttattttt taatagaaat tgaggcaaaa tattttatga
      241 aaaaaatgag attactttcc tggaatgtta acgggatccg agctattcaa aaaaaaggat
      301 ttgttgattg gttattaact gaagatcccg atattctttg tctgcaggaa actaaagccg
      361 cacctgaaca attatctgat gaattattaa acatcaacgg atacaaatct tatttctctt
      421 cttcaatagt taaaaaagga tatagcgggg ttgcaatcta ttcaaagcat gaaccattaa
      481 aagttgataa cggttttggc attccaaagt ttgatgacga gggaagaatt ataatcgcgg
      541 attacaaaga ttttgtactg ctaaacattt actatccaaa cggaaaaaaa aatgaagagc
      601 gattgcagta taaaatggat ttttacgatg catttcttga ttatacaaat aagcttgtta
      661 agaaaggaaa gaatcttgtt atttgcggtg atgtaaatac agctcacaaa gaaattgatt
      721 tagcaagacc caaagaaaat tctaaagtat caggttttct acctatcgaa agagaatggc
      781 tggataaatt tttctcacat aattatcacg atacatttag actgtttaat gatgaacccg
      841 aaaattacac gtggtgggat caggtaacgc gtgctcgcga tagaaacgtt ggctggagaa
      901 tcgattactt ttttgtttcc gataatttta agaaaaatgt aaaagatgcc tttataatgc
      961 ctgatgtaat gggctcagat cattgtcctg ttggagttga tatagagatt aaataaccgt
     1021 attgaatttt tttatttgaa ttgttgttaa atcttcaaat taatttttta ttctaaacta
     1081 atttgatgaa acacatatac acgacaatta gtctttattt tatgtttgca gtaattgttt
     1141 ttttgcagat tgtaactcct gcacagcaaa aatcacttgc tgaagcaaaa cttgtcgttg
     1201 attcattttc tccacagaaa gactcctcta cagatgaaat tttgaattat ggttacgggc
     1261 acaaggtttt gtttattagt gaaatattta ttccaaacga ttctcctaga aaagaaatat
     1321 ctatatcaac aaaactcaca tcactaatct gtataagcaa gcaaagttca actccttacg
     1381 gatgcagtgt aaaatattaa tggttaactt aattaattaa atatttataa ttttgtaaaa
     1441 agtgataaac gaaaaaagag tccgccttct tcaaaaagga aatgagacca aaggcccaat
     1501 tgtctattgg atgagccgcg atcaacgggt tcatgataat tgggctttgt tatttgccca
     1561 gaagttagct ttagaaaagc aaagatcatt agcagtagtg tttagtcttg tgcctgattt
     1621 tcttgaatca acaatacgcc aatacggatt tatgctaaaa ggattgcaag aagcagaatc
     1681 agaattatct aaacataaca tacctttttt tctaacatca ggtgagccgg aagtagaaat
     1741 tcctgagttc ataaaaaaca ttaatgcttc aattattgtt tcagattttg atcctcttaa
     1801 aatcaaaaga atctggaagc gcgatgtggc caaacaaatt tcaattccat tctatgaagt
     1861 tgatgcacat aatattgttc cttgtttgca tgtttctgat aagttagaat ttgccgctta
     1921 tacaattcgt cctaaaatcc acaaagcatt aattgagttt atggatgaat ttccatcatt
     1981 aaagaaaatg agaaagagtg aaatttcatc tgataaaatt gattggacta aaattcaaaa
     2041 atcactaaaa attaattttg aagtaaaaga agttgaatgg attaaacccg gagaagttga
     2101 agcttcaaaa actttagagc attttttgaa gaacaaattt gaaaattatc acgaattaag
     2161 aaacgatcct acaaaagaat atcaatcaaa tctttcgcct tatttgcact tcggacaaat
     2221 ttctgctca
//
LOCUS       DASWBY010000552         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_284034, whole genome shotgun sequence.
ACCESSION   DASWBY010000552 DASWBY010000000
VERSION     DASWBY010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284034"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VF870_09930"
     CDS             complement(<1..582)
                     /locus_tag="VF870_09930"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9252551.1"
                     /translation="MKFKLILIFFELIILAIYPQQISSPDSKLTLKIKLSENNEFVYN
                     LIREGKVVVNDSKLGIDLLDQPDFLNGFKIDAIVTSNYFEEWSPVWGEVDKIVNNYNQ
                     MQVTLIQPKEKRKMVITFRLFNDGLGFRYEFPAQDNLKYFIVLEEDTQFALTGDHKTF
                     WIPGDYDSQEYSYTSSQLSGIHSEKGKSADEILV"
     gene            complement(617..1933)
                     /locus_tag="VF870_09935"
     CDS             complement(617..1933)
                     /locus_tag="VF870_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX9252552.1"
                     /translation="MRFLFLFICLFTTSNFTSIYSQIFSEEPVLRVRIIYTVDTLNLT
                     FLDEWNISRSDSQENLKISRDDSILIFIQNDSIALNNYSGNNTGYYNNIFLKAVDSAG
                     TLKIKNVPYGIGWWWQGTEDRIYEGDFEISINENNKLDVIVSLPLEKYLCGVVPYEIG
                     NDAPLEALKAQVIAARSETVSALLSGQYKGSNFDLCADVECQVFAGNNKRTTITDKAV
                     EDTRGLCLFYGDEVIGAYYASNCGGISENVENVWPDRSGPVPYWSSHFDSDEAQNFNP
                     KNNPEKWINSNPDVFCNPFFHPELPEWSKKNFRWKVEMTNDELTNNLNELKSIGELRE
                     IKIIERGNSGRIIKARFIGSRDSLDLNSELDIRKIKKPPFRSSCFVVDKILDDQRNDK
                     YLFNGAGWGHGVGMCQSGAVARAFLGQSFIQILNHYFPETEIKTVY"
BASE COUNT          694 a          392 c          308 g          718 t
ORIGIN      
        1 aactaaaatt tcatctgcac ttttaccttt ctcagagtga attccggata attgtgagga
       61 tgtgtatgag tattcctgag aatcataatc gcccggaatc cagaatgttt tatgatctcc
      121 tgttagtgca aattgtgtat cctcctcaag aacaatgaag tattttaaat tatcctgggc
      181 aggaaattca tacctaaagc caagcccatc gttaaaaagt ctgaaagtaa taaccatctt
      241 tcttttttct tttggctgaa tgagagttac ctgcatctga ttataattat ttacaatctt
      301 atcaacttca ccccaaacag gcgaccattc ttcaaaataa tttgaagtta caattgcatc
      361 tattttaaaa ccatttaaaa aatcaggctg atccaaaaga tcaattccta atttgctgtc
      421 gttcactaca actttacctt ctctgatcag attatatacg aactcattat tttcagaaag
      481 ttttattttt aaggtaagtt ttgaatcggg tgaagaaatt tgctgtggat agattgctag
      541 aattattaac tcaaaaaaga taaggattaa tttaaatttc ataagtagta cctttcttca
      601 cacatcataa aataattcaa tagactgttt taatttcagt ttcagggaag tagtgattca
      661 atatttgtat aaacgattgt cctaaaaaag cccgtgcaac agcaccggat tgacacattc
      721 caacaccatg gccccagccg gcaccattaa ataaatattt atcatttcgc tgatcatcca
      781 aaattttatc gactacaaaa caagaactac ggaaaggagg ttttttgatt tttcttatat
      841 caagctcaga atttagatcc agagaatctc tgcttccaat aaatcttgct ttaataattc
      901 tacctgaatt tcctctttca atgattttaa tctctctcaa ttctccaata gatttaagtt
      961 cattaagatt attagttaat tcatcgtttg tcatttccac cttccagcga aaattctttt
     1021 tgctccactc cggtaattcc ggatgaaaaa atggattaca aaaaacatct ggattactat
     1081 ttatccactt ctccggatta ttttttggat taaaattttg tgcttcatca gaatcaaagt
     1141 gtgaactcca atagggaaca gggccagatc tgtcaggcca gacattttca acattttcag
     1201 aaattcctcc gcagtttgaa gcataataag ctcctatcac ttcatcacca taaaacaggc
     1261 acaatcctct tgtatcttca acagctttgt cggttatagt tgttctttta ttattgcctg
     1321 caaacacctg gcattcgaca tcagcgcaaa gatcaaaatt gcttcctttg tattgacccg
     1381 aaagcaatgc agaaacagtt tcagatctgg ccgcgataac ttgtgcttta agtgcttcaa
     1441 gaggtgcgtc attaccaatt tcataaggaa cgacgccgca taaatatttt tctaaaggca
     1501 gcgacacgat tacgtcaagc ttattattct catttattga aatctcaaaa tcgccttcgt
     1561 aaatgcggtc ttcagttcct tgccaccacc atccaatgcc atagggaaca tttttaatct
     1621 tcaaagtgcc agcagaatca actgctttaa gaaaaatatt attatagtaa cctgtattat
     1681 ttcctgaata attatttaat gctattgaat cattttgaat aaatattaat atagaatcat
     1741 ccctgctgat ttttaaattt tcttgtgagt cagatcgaga tatattccat tcatctaaaa
     1801 aagtcagatt aagagtatca acagtataga ttatcctgac tcttagaacc ggttcttctg
     1861 aaaatatttg tgaatagatc gaagtgaaat ttgaagttgt gaataaacag atgaataaaa
     1921 aaaggaatct caatttatat ctctttacct gtaaagtgtt tccaaaaagc taaaatcaga
     1981 gtactgaatt tataagaatt aaaataaact gtcgttgtta aaatacttta gttttaattg
     2041 tgatatttaa ccagtaatag ttttctgcta acttttttta tcaacaatat ctgctaacag
     2101 attaagcagc tg
//
LOCUS       DASWBY010000553         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_56880, whole genome shotgun sequence.
ACCESSION   DASWBY010000553 DASWBY010000000
VERSION     DASWBY010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56880"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VF870_09940"
     CDS             <1..342
                     /locus_tag="VF870_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9252553.1"
                     /translation="ASANESISEALLQQTKNEIRLKLKNAHTSYINNQQQIKTYNDDI
                     LPQTEEVYRTAIKSYDAGELSYLEYMQVKQLVINARENYITALFNYNVSLFELEEIIG
                     KNIYELENKND"
     gene            335..1510
                     /locus_tag="VF870_09945"
     CDS             335..1510
                     /locus_tag="VF870_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9252554.1"
                     /translation="MIDKKLYTLIVIMILTLLQIGCTEKPEEHNEEHNEEHSDLITLS
                     SESIEQIKLKSQTVSLQPFSGFLNIPAEVKTNQDNEAQIGSLIQGRVHKVFVKVGDYV
                     KAGQLLMTVEGLEVGEIKAGYLKAKANYDNAKANYERQKKLFDEKIGSQKSLLESQAE
                     YEKATAEYRAEDKKIHSVGLSDEDVINEKDGDEHTSGTLSIKSLINGVVVERNVVIGQ
                     VVDATTNAFKVINTSNVWIDGQIYEKDLDKITQNSKAYFTTSTNKQNRLEGRIIYIGQ
                     TVDDNTRTILIRGEFVNQGNKLKPQMYGELQIPVGSNAKAILIPEESIVKEAGQSYLF
                     VQTSDTTFERRVVIAGTTSGNLIEIKEGVKEGEKIVTDGVFYLKSELKKDELAEDEH"
     gene            1510..>2504
                     /locus_tag="VF870_09950"
     CDS             1510..>2504
                     /locus_tag="VF870_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9252555.1"
                     /translation="MLEKIIEYTLHKKGMIIFLSLVILAFGAYSYLTLPIDAFPDVTN
                     IQVEIVSHADGLSAIEIERNVTYPIETSMRGLPDVEQMRSVTKFGLSIVTIIFKDDVD
                     IYFARQLVFERLAEAKESVPEGVEIEMGPIATVMGEIYQYTLEGNVPADSLEKISYLS
                     NLRTIQEWIVTPQLKSVAGVNEINSFGGYFKQYQVILSPDALIKYNLTTDQIFTAIEN
                     NNQNVGGNILEKNSDQYIVRGVGLIKDISDIENIVLKSYDGTPIYLRDAAKIKVGEAV
                     RMGAAMINGKDECVGGIVMMLRGENSRDVVDRVKNKVEEINSNNVLPDGIKLVPY"
BASE COUNT          963 a          337 c          528 g          676 t
ORIGIN      
        1 gccagtgcaa atgaatcaat ctccgaagct ttactacaac aaacaaaaaa tgaaataaga
       61 ttaaagttaa aaaatgctca cacgagttat atcaataatc aacagcagat aaaaacatat
      121 aatgatgata tacttccgca gactgaggaa gtttatagaa ctgcaataaa aagctatgac
      181 gctggcgaat tatcttatct agaatatatg caggttaagc agctggtgat aaacgcaaga
      241 gaaaattata taacagcttt gttcaattac aatgtatcgt tgtttgaatt agaagaaata
      301 atcgggaaga acatttatga attggagaat aaaaatgatt gataaaaaat tatatacgct
      361 gatcgtgata atgattttaa ctttattaca gattggatgc acagaaaaac cagaagaaca
      421 taatgaggag cataacgaag agcattcaga tttgataaca ctttcgtcag aatcaattga
      481 gcaaataaaa ttaaaatcac aaacggtatc gttacagcca ttcagcggtt ttcttaatat
      541 ccctgcagaa gtaaaaacta accaggataa tgaagcacag attggatcat tgatacaagg
      601 aagagtgcat aaagtatttg ttaaagttgg tgattatgtg aaggccgggc aactcttgat
      661 gactgttgaa ggacttgaag ttggagaaat aaaagccggt tacttaaaag ctaaggctaa
      721 ctatgataat gcgaaagcaa attacgaacg acagaaaaaa ctatttgatg aaaaaatcgg
      781 gtcacagaaa tctctattag aaagtcaggc cgagtatgag aaagctactg ccgagtatcg
      841 tgcagaggat aaaaagattc attcagttgg actaagtgat gaggatgtta taaatgaaaa
      901 agacggggat gaacatacct ccggtacttt gtcgattaaa tcacttataa atggagttgt
      961 tgttgaaaga aatgttgtga ttggacaggt tgttgatgcg accactaatg cctttaaagt
     1021 aataaatacg agtaatgtct ggattgatgg tcaaatctat gagaaagatt tggataagat
     1081 tactcaaaat tcaaaagcgt actttaccac cagcacaaat aagcaaaaca gacttgaggg
     1141 cagaataata tatattgggc agactgttga tgataacacc aggacaatct taattagagg
     1201 tgaatttgta aatcagggca acaaattaaa acctcaaatg tatggtgaat tacaaatacc
     1261 tgtggggtca aatgcaaaag caattttgat tcctgaagaa tcaatagtaa aagaagcggg
     1321 acaaagctat ttatttgttc aaacaagtga tacaactttt gaaagaagag ttgtgatagc
     1381 cggaacaaca tcaggcaatt tgattgaaat aaaagaaggt gttaaggaag gtgaaaaaat
     1441 agtaactgat ggtgtgtttt atctaaagag tgaattgaag aaagatgaat tagcggagga
     1501 tgaacactga tgttagaaaa aataatagaa tatacattgc ataagaaagg aatgataatt
     1561 tttctttcac ttgtgatatt agcatttggt gcatactctt atttaacatt accaattgat
     1621 gcatttccgg atgtcacaaa tatacaagtt gaaattgtaa gccacgcaga cggactatct
     1681 gcaatagaaa tagaaagaaa cgtaacctat ccaatcgaaa cctcaatgcg tggattaccg
     1741 gatgtagaac agatgcggtc ggttacaaag ttcgggttat ccatagtaac aataatattt
     1801 aaggatgatg ttgatatata ctttgcaaga cagcttgtat ttgaacgatt agcagaagca
     1861 aaggaaagtg ttcctgaagg cgtggaaatt gagatggggc ctattgccac cgtgatgggt
     1921 gaaatatatc aatatacact tgaagggaat gtacctgctg attcgctgga aaaaatatct
     1981 tatctgtcaa atctgagaac aatccaggaa tggattgtaa caccgcagtt aaaaagtgta
     2041 gcgggagtaa atgaaataaa ttcatttggc ggctacttta agcaatacca ggtaatatta
     2101 tcacctgatg ctttaataaa atataatctg actactgatc aaatctttac tgctatagaa
     2161 aacaataatc agaatgttgg cggtaatata cttgagaaga attcggatca gtacattgta
     2221 cgaggtgttg gactaataaa agatatttca gacatcgaaa atatagttct gaaatcatat
     2281 gatggtacgc caatatatct tagggatgca gcaaaaataa aagttggtga agcagtaaga
     2341 atgggtgctg caatgattaa tggaaaagat gagtgtgttg gcggaattgt tatgatgctt
     2401 aggggtgaaa acagccgaga tgtagttgac agagttaaaa ataaagttga ggaaatcaat
     2461 tctaataatg tactaccaga tggaataaaa ttagtacctt acta
//
LOCUS       DASWBY010000554         6508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_284213, whole genome shotgun sequence.
ACCESSION   DASWBY010000554 DASWBY010000000
VERSION     DASWBY010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284213"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1455)
                     /locus_tag="VF870_09955"
     CDS             complement(91..1455)
                     /locus_tag="VF870_09955"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9252556.1"
                     /translation="MKIMLVEDEKITRKTLTDILKKENYDVSSYADGTEALNALKQNN
                     FDTVITDLRLPGTNGLDILKAAKEIDPRTIVIIITAYATVETAIDALKLGAYDYLLKP
                     FSPEQLLSILKHIEQLSIVLNENEKLKSRLELFEKKTIIGSSKKVIAFLDMIKMVAPQ
                     DYTILIQGESGTGKELCARTIHNLSLRKDKEFIAINCAVIPEALIESELFGHEKGSFT
                     GAVKRHVGYFEKANGGTLLLDDIDDLPLPIQVKLLRVLQEKEISRVGSTENIPIDVRI
                     ICATKADLKKRVQQKFFREDLYYRLNIIPIILPPLRERKEDIPELLDHFLKKHGAEDK
                     VKTVNENIYSILNQYDWPGNVRELENITERIIALSPNQNLNISVLDSILINEKKEKLL
                     DNQIDEYDGLDKYILAKEREIISWALSKSGNNVSGAAKILKIPRTTLRSKMDKLSMVT
                     LPEV"
     gene            complement(1436..2911)
                     /locus_tag="VF870_09960"
     CDS             complement(1436..2911)
                     /locus_tag="VF870_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9252557.1"
                     /translation="MIPIQHSLKSRILFVTGSVILLIMMIISLAILFQWRKILIEKEV
                     INAQSIVDAFTVPALDALIYAEQRELLKDDLLETYIDNYMQRVNSIKYIEIYDNNLEI
                     VAGKDRNGSSSFSLNRSDENLKLNRYESIIYKSAEYGWLLDTYAPLKIANKKWGVVKI
                     GFDAEPIKDEINDIFFLLLSLTVLLSIATLTVLYFLIDKVTFNLKNLVDQIDKIDFKS
                     IEEISLKGNKDEIGFLINRFDDLLHRLKLSKQQLAIAQKQVYQSEKLASIGRLASGVA
                     HEINNPLNGIKNCLYAIQKEPDNKEQTTEYLALINEGMNYIENVINKLLGFARQQTQI
                     TVSLNINEMILNIYKLLEYKLKQKQALVNFNLLDGIPVISADNQLIQEVIMNIFINAY
                     DAISEQGEIEIKSGIWDNKHIYFSISDNGIGIQENEIEKIFDPFYSTKDPGKGTGLGL
                     WVSLGIIENHNGQITVKSIPNKETIFTVILPTEVKYENNVS"
     gene            complement(2908..3867)
                     /gene="phnD"
                     /locus_tag="VF870_09965"
     CDS             complement(2908..3867)
                     /gene="phnD"
                     /locus_tag="VF870_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9252558.1"
                     /translation="MDIKRILNNSSFMLRKEFLISLLIFIVIGFAVTFLFYNSAIDIS
                     TKILADKLELNSKSEKNKSTVFVGVISRYPPNIIFKGYQPIIDYLNQNDKFHFELKLS
                     TSYSETVNQLINGDVAIAFFGSLLYTKVHDLYGITPILKPLSEDHKPFFKSVVFTKST
                     SNINSIKDLKGKRIALPSNESFSGNWLIKYELRESNVFEKDIKEFKNFSYHQNVIYQV
                     LNGNFDAGVVKDRVIKEYENRGIKIIGYSEPVPGSPIVAARNYDKEIINTVKSLLLKI
                     DLNNIKYQELVKTWDKEFTFGFIEANDSDYDLIRKYSGEQKKK"
     gene            4114..4698
                     /locus_tag="VF870_09970"
     CDS             4114..4698
                     /locus_tag="VF870_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252559.1"
                     /translation="MENIVPRWEWRTFTNDLSTAEENIRKHPEGKTRESDEVYILSEV
                     SMDNTKVRDNLMDIKTLQQVNEDKLEQWLPIMKGTFPLPVSEIEKVFKCFKVALPKFE
                     RTEYTFQQYLDEVIKPSKLLKAVNVHKKRTGFTINNTIVEIAEVTVDGKVIKTAAVEM
                     EDPTLVIKTVRELELDKFPNINYLRGLKNLVGMK"
     gene            4711..5178
                     /locus_tag="VF870_09975"
     CDS             4711..5178
                     /locus_tag="VF870_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX9252560.1"
                     /translation="MANEIERKFLVKGEFKNLASKETRIVQGYLSSVPERTVRVRIKG
                     DKGFITIKGIGNASGAARYEWEKEIPIGEVEELLKICEPGVIDKTRYLVKVGNHTFEV
                     DEFYGENKGLTIAEIELSSESEAFVKPVWLGDEVTGDTKYYNSMLMKNPYTKW"
     gene            5229..>6508
                     /locus_tag="VF870_09980"
     CDS             5229..>6508
                     /locus_tag="VF870_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX9252561.1"
                     /translation="MTKFDPLDMNNYRVEKLPKRDKSKFEKWLTIIGPILALAAFILF
                     AYIIKLPFLETINPSQIVSESAKKTYEKLGSAVFIRNNEFMLAIFVASIILWMTQAIP
                     NYLTSLMVIISLVITGVLSEKEAYAQLGHPVMWLNIMSFVLASMLVVTGLAKRFALWL
                     IIKFGKNASTIFITFLIINLVLSAFISATTAKAAILLPIFMVIAAIYGAKGGANKNNF
                     GRSIVLQNLLYINIGAGAFLTGSGANLLAAAIIGGAIGGNIFFSEWMMAMFPVVLCLM
                     FIGYLLAMKIFFPLSPEERLPQIEGGMDRLKQEYDKLGSLTFLEIKSSIIFVIILGMW
                     ATDSIHGISPTAVAFFGAIVALMPKIGIVKWNDVDIPWHLMLFSAGAYTLGAGLDVTD
                     LPSISVNAFFDHLGIGDNTQFWVLYLLLTGVMVF"
BASE COUNT         2129 a         1021 c         1023 g         2335 t
ORIGIN      
        1 tagaaataat tgatcagcaa acttttattg atgatgtgaa taggaattgg atttaccaat
       61 tctagattat taaaatacat taaaattatt ctacacttca ggtaaagtta ccatagataa
      121 tttatccatc ttacttctta aagttgttct tggaattttt aagattttag cagcaccaga
      181 aacattattt cctgacttgc ttaaagccca ggatataatt tctctttcct ttgctaatat
      241 atatttatca agtccatcat actcatcaat ttgattatca agtagctttt cttttttttc
      301 gttaattaaa attgaatcaa gaactgatat gttcaaattt tgatttggag agagtgcaat
      361 aattctttcg gttatattct caagttctct cacgttgccc ggccaatcat attgattaag
      421 aattgaataa atattctcat taacagtttt aactttatcc tcagcaccat gttttttaag
      481 aaagtgatca agtaattcag gtatatcttc ctttcgttca cgaagcggtg ggagtataat
      541 tgggataata tttaatctat aatatagatc ttcccgaaaa aacttttgtt gcactcgttt
      601 ctttaaatct gctttagttg cacatataat tcgcacatca attggaatat tttctgtgct
      661 tccaactctg gatatttctt tttcctgtaa aactcttagt agcttaacct gaataggtag
      721 aggcaaatca tcaatatcat caagaagcaa agttccgccg tttgctttct caaaatatcc
      781 cacgtgtctt ttaacagcac cagtaaatga acctttttca tgtccaaata attcactttc
      841 aattagtgct tctggaatta cagcacaatt aattgcaata aactctttat ccttccgcaa
      901 agaaagatta tgtattgttc ttgcacaaag ttcttttcca gttccgcttt ccccttgtat
      961 taaaatagta taatcctgcg gtgcaaccat tttaatcata tctagaaaag ctattacttt
     1021 tttagatgag ccaataatag ttttcttctc aaatagctct aatctgcttt ttaatttttc
     1081 attttcatta agtactatac taagctgttc tatatgtttt aaaatactta gcagctgttc
     1141 cggagaaaag ggttttaata gataatcata agctccaagt tttaatgcat caattgcggt
     1201 ttcgacagtt gcataggcag taataataat aactattgtt ctcggatcta tttcctttgc
     1261 tgcttttaaa atatctagtc catttgtacc aggtaatctt aagtcggtaa taactgtatc
     1321 aaaattattt tgctttaatg catttagtgc ttcagttcca tctgcgtaac tgcttacgtc
     1381 ataattttct ttttttagaa tatcggttaa agttttacga gtaatcttct catcttcaac
     1441 taacattatt ttcatatttg acctcagttg gtagaattac agtaaaaatt gtttccttat
     1501 ttggtataga tttaacggtt atttgtccgt tatgattttc aattattcct aagctaaccc
     1561 atagtcccag acctgttcct tttccaggat ctttcgttga ataaaaggga tcaaaaattt
     1621 tttctatttc attctcttgt attccgatgc cgttatcact tatagaaaag tagatgtgct
     1681 tgttatccca aattccagat ttaatttcaa tctcaccttg ttctgaaatt gcatcatatg
     1741 cattaataaa tatattcata attacttcct gaataagttg attatcagcg cttattacag
     1801 gaatgccatc taacaggtta aaatttacaa gagcttgttt ctgctttaat ttatattcaa
     1861 gtagcttata aatgtttagt atcatttcat taatgttaag cgaaactgta atctgtgttt
     1921 gttgtcttgc aaaacctaag agtttgttaa taacattttc aatgtagttc ataccttcgt
     1981 taattaaagc caaatattct gttgtttgtt ctttgttatc aggttctttc tggatagcat
     2041 acaaacaatt cttaatgccg tttaatggat tatttatttc atgcgcaacc ccggatgcca
     2101 gtcttcctat agatgcaagt ttttcggatt gatatacttg cttttgtgct attgccaatt
     2161 gttgtttaga aagttttaat ctgtgcaata aatcatcaaa acgatttata agaaatccta
     2221 tctcatcttt atttcctttt agggaaattt cctcaattga tttgaaatcg attttatcaa
     2281 tctgatcaac aagatttttt agattaaacg tcactttatc gatgagaaaa tataaaacgg
     2341 ttaatgtagc tatagaaagt aagacggtta aagaaagtag aagaaaaaag atatcattta
     2401 tttcgtcctt aattggttca gcatcaaaac caatttttac aactccccat tttttattag
     2461 caatttttag cggagcataa gtatccagta gccatccata ctcagcagat ttataaatga
     2521 tactttcata tctgttcaat tttaagttct catctgatct gttcaatgaa aatgaacttg
     2581 atccatttct atcctttcca gctacaattt ctaaattatt atcgtatatc tctatgtatt
     2641 ttatactatt tactctctgc atataattat caatataagt ttcaagtaaa tcatccttta
     2701 ataattcacg ctgttccgca tatatcaaag catcaagagc tggaactgta aaagcatcta
     2761 ctattgattg agcatttatc acttcttttt ctattaatat ctttctccat tgaaacagaa
     2821 ttgccaatga aataatcatc attattaaaa ggataactga accggttaca aaaagtatac
     2881 gtgactttag actatgttgg attggaatca tttctttttt tgttccccag aatattttct
     2941 tatcaaatca tagtcgctgt cattggcttc aataaatcca aaagtaaatt ccttatccca
     3001 tgttttaaca agttcttgat acttaatatt atttaaatca atttttagaa gaagtgattt
     3061 aactgtattg attatttctt tatcataatt tcttgcagca acaataggtg aacctggtac
     3121 aggttcagaa tacccgataa tttttatacc tctattttca tattctttta taactctatc
     3181 cttaacaaca cccgcatcaa aatttccatt taaaacttga taaattacat tttgatgata
     3241 agagaaattt ttaaactctt taatatcctt ttcaaaaaca ttgctctctc gtagttcata
     3301 ttttattaac caatttccgg aaaatgattc gttagacggt aatgcaattc ttttaccttt
     3361 caaatcttta attgaattaa tattgctggt tgattttgta aataccacgg acttaaagaa
     3421 gggtttatga tcttcactaa gtggttttaa tattggagtt ataccataaa gatcatgaac
     3481 ttttgtgtaa agaagtgaac caaaaaatgc gattgcaaca tcaccgttaa taagttgatt
     3541 tacggtttca gaatatgatg tactaagctt aagttcaaaa tgaaatttat cattttggtt
     3601 taagtaatct atgattggtt gatagccttt gaaaataatg ttaggtggat atcgagaaat
     3661 aacgcctaca aaaacggttg acttattctt ttccgactta gaatttagtt ctagtttatc
     3721 agccaatatt tttgtggaga tatctattgc agaattataa aatagaaatg ttactgcaaa
     3781 tcctataaca ataaaaatta atagtgaaat taaaaattct tttcttagca taaatgaaga
     3841 gttattcaaa attcttttaa tatccatcgt caaatatcag tcctagttgt aatttcaaat
     3901 tcattattaa tttcaatcca aacctaaaca aaatgacgaa taatcgccac actgacgacg
     3961 ataaatcgcc taaagttctt ttaagagttt tagtggaata ataaaatgat gattttaagc
     4021 tttatttgaa tcatacaatc ataattttat ttgatcgctt agggcattat ccttgataaa
     4081 aagtatcaac tatttcaatc aaaaggagta aaaatggaaa atatcgtacc tcgttgggag
     4141 tggcgcacat ttacaaacga tcttagtaca gcagaagaaa atattcgaaa acatcccgag
     4201 ggtaaaactc gagagagtga tgaagtgtat attctttcgg aagttagtat ggataacaca
     4261 aaagtgcgcg ataatttgat ggatatcaaa acccttcagc aagttaatga agataaatta
     4321 gaacaatggc tgcctattat gaaaggaaca tttcctctac ctgtttcgga aatcgaaaaa
     4381 gtatttaaat gctttaaagt tgctcttcca aagtttgaaa gaacagaata tactttccaa
     4441 caatatttag atgaagtcat aaagccctct aaattattaa aggccgttaa tgtgcataaa
     4501 aaaaggacag ggtttacgat taacaataca atcgttgaaa ttgcagaggt tactgttgat
     4561 ggtaaagtga ttaaaaccgc tgctgttgag atggaagatc ctactttggt tataaaaact
     4621 gtaagagaac ttgaacttga taaatttcca aacattaatt atttgcgtgg attaaaaaat
     4681 ttagttggaa tgaaataagg aataaaaatt atggcaaatg aaatagaacg taaattttta
     4741 gttaagggtg aatttaaaaa cctcgctagt aaagaaacca ggattgtaca aggatatctt
     4801 tcatctgtac cggaaagaac tgtgcgcgtt cgaataaaag gtgataaagg atttattaca
     4861 ataaaaggta taggaaacgc ttccggcgca gcgagatacg aatgggaaaa agaaattcca
     4921 attggtgaag ttgaagaact acttaaaatt tgcgaacctg gagtaataga taaaacacga
     4981 tatttagtaa aagttggaaa tcacactttt gaagttgatg agttttatgg tgagaacaag
     5041 ggattaacaa ttgcagaaat tgaattaagt tctgaatcag aagcatttgt aaaacctgtg
     5101 tggttaggtg atgaagtaac aggtgatact aaatattata attctatgct catgaaaaat
     5161 ccttacacaa aatggtaagg tgaattaaca aattatcata aaacttttta acatttaata
     5221 aaactgatat gacaaaattt gatcctctcg atatgaataa ttatcgagta gaaaaactcc
     5281 caaaacgaga taaatcaaaa tttgagaagt ggcttaccat aattggtccg atcttagctt
     5341 tagcagcatt tatactattt gcttacataa ttaagcttcc atttctggaa acaatcaatc
     5401 cttcgcagat agtctcagag tcagcaaaaa aaacttacga aaaactaggc tctgcggtat
     5461 ttattagaaa caacgaattt atgcttgcaa tttttgttgc gtctataatt ctatggatga
     5521 ctcaggcaat cccaaattat ttaacatcat tgatggttat aataagtttg gttataactg
     5581 gtgtgttatc ggaaaaagaa gcttatgctc agcttggtca tcccgtaatg tggttgaata
     5641 ttatgtcatt cgtgctggca agtatgttag ttgttactgg gctcgctaaa agatttgcgc
     5701 tctggttaat aattaaattt ggtaaaaatg ccagtacaat ttttataact ttcctaatca
     5761 taaatcttgt cctctcagct tttatatcgg caacaacagc aaaagccgca atactcctac
     5821 caatctttat ggtgatcgca gctatatatg gtgcaaaagg tggtgcaaat aaaaataatt
     5881 ttggtcgaag tatagtattg cagaatctat tatacatcaa tattggtgca ggtgcatttt
     5941 taacaggatc gggagctaat ttattagcag ctgctattat tggtggtgca attggcggaa
     6001 atattttttt ctcagaatgg atgatggcaa tgtttcccgt tgtactttgc ctaatgttta
     6061 ttgggtattt acttgcaatg aaaatattct ttcctctttc gcctgaagaa cgattaccgc
     6121 aaattgaagg tggtatggat cggttaaagc aagaatatga taaacttggt tctttaactt
     6181 ttctggaaat caagtcctca attatttttg ttataattct tggcatgtgg gcaacagata
     6241 gtattcatgg aataagtcct actgcagttg cgttctttgg agctatagtt gctttaatgc
     6301 ccaaaatcgg gattgtaaaa tggaatgatg tcgatattcc ttggcatctt atgttatttt
     6361 ctgcaggagc ttatacttta ggcgcaggtt tggatgtaac tgatcttccc tctataagcg
     6421 ttaatgcctt cttcgatcac ttgggtattg gtgacaatac gcaattctgg gtgctttatt
     6481 tattgttaac cggagtaatg gtttttag
//
LOCUS       DASWBY010000555         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_737443, whole genome shotgun sequence.
ACCESSION   DASWBY010000555 DASWBY010000000
VERSION     DASWBY010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737443"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VF870_09985"
     CDS             complement(<1..300)
                     /locus_tag="VF870_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9252562.1"
                     /translation="MKKNIDIAKLAKGFKGKIIQAADSNYDDARKIWNAMVDCKPALI
                     AQVSDADDVSTAIRFARETNLDITIRGAGHNIAGNSICNDGLMIDFSNMKNVNVDI"
     gene            complement(375..665)
                     /locus_tag="VF870_09990"
     CDS             complement(375..665)
                     /locus_tag="VF870_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252563.1"
                     /translation="MYLVRDTFNCKPGMAKELVKKFKQTIPFMEEQQLKNVRIMTDVV
                     SNYWTVVLEGEVNSLAEFENRRGFTSQPKVQEIMKDYMTLVEGGKREIFQIE"
     gene            complement(741..1865)
                     /locus_tag="VF870_09995"
     CDS             complement(741..1865)
                     /locus_tag="VF870_09995"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX9252564.1"
                     /translation="MKKKSIKILFLFLLSTLVFYSCNDAVLPTDSNINNSQITEKKQT
                     DNVSISVFASGLNSPRGLKFGPDGYLYVAEAGLGGNNSTLGQCDQVIPPIGPYLGGNT
                     ARILKINSSGSISTVVDNLPSSQNAMGDLLGAADIEFINNDLYALIAGGGCSHGHSDV
                     PASIIKINSDASWSVLADLSTYQQNNPVAHPEEDDFEPDGSWYSLINVRGNLYAIEPN
                     HGEMVKVSTNGNIKRVIDFSASYGHIVPTSVAYHGNFFVGNLNTFPTVAGSSNIYKVT
                     PSGQSQIWATGFSTILGVAFDKRDNLYVLETSSVDGFPTPETGKIIKVSPSGLKSVIV
                     DNLFFPTGMTFGPDGALYVSNKGFGPPIPGLGEILRIAVN"
     gene            complement(2031..2630)
                     /locus_tag="VF870_10000"
     CDS             complement(2031..2630)
                     /locus_tag="VF870_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HEX9252565.1"
                     /translation="MKKSVFISASIAMISLLILSQFNCSGREETKQMTQQELIARGKY
                     LVTTGGCDDCHTPKIYTAKGPVPDTTKRLSGFQQGEKLPALDVKYVAPGNWVATESNF
                     SGWVGPWGISYASNLTPDNSTGIGALTEEMFIKTLREGKYMGVGRPLLPPMPWPTIGQ
                     MTNDDLRSIYAYLRTIKPIHNEVPQPTPPDKLMELLAGK"
BASE COUNT         1018 a          554 c          510 g         1094 t
ORIGIN      
        1 tatatcaaca tttacgtttt tcatatttga aaaatcaatc ataagaccat cgttacaaat
       61 tgaattgcca gcaatattat ggccagcccc acgaattgta atatctaaat tggtttccct
      121 ggcgaatcta atcgcagtag aaacatcatc agcatctgat acttgtgcaa tgagcgctgg
      181 tttacaatcc accattgcat tccatatttt tcttgcatca tcataattac tatctgctgc
      241 ttgaataatt ttccccttaa aaccttttgc taacttagca atatctatat tttttttcat
      301 ataatctctt tattaattta tttaatattt ttagtggagc ttataacaat tttttacgtt
      361 aacgacttaa ttggttattc aatttgaaaa atttctcgtt taccaccttc aacaagagtc
      421 atatagtctt tcattatttc ctgaactttc ggttgtgaag taaaacctcg tctattctca
      481 aattctgcta agctattcac ttcaccctca agaactacag tccagtaatt tgaaactacg
      541 tcggtcatta ttctaacatt ttttaattgc tgctcttcca taaaaggaat tgtttgcttg
      601 aatttcttga ctaattcttt tgccatcccg ggtttgcagt taaaagtgtc tcttactaaa
      661 tacatattat ctccttcaaa atagattaat catttaatga aaaatgtctg ctacttaagc
      721 agacattttc cgataataaa ctaatttaca gctatcctta aaatttctcc taaacccgga
      781 attggtggcc caaatccttt attggaaaca taaagtgctc catctggtcc aaatgtcatt
      841 cctgtgggga aaaataaatt atcaacgatt actgacttta aacctgaagg agaaacctta
      901 ataatcttac cagtttctgg agtagggaat ccatccactg aagatgtttc taagacataa
      961 aggttatcgc gtttatcaaa tgcaacaccc aatatggtag aaaaaccagt cgcccaaatt
     1021 tgggattgtc cgctcggagt cactttgtaa atattggagc tgccggcaac agttggaaat
     1081 gtattaagat taccaacaaa aaagttacca tgataagcta cagaagttgg aacaatgtga
     1141 ccataagaag cactaaaatc aattacacgt tttatgtttc catttgtcga tactttaacc
     1201 atttcaccat gattaggttc aatagcataa agattacctc ttacattgat aagactgtac
     1261 cagcttccat ccggctcaaa gtcatcttct tccggatgag ctacaggatt gttctgttga
     1321 taggtgctga gatcagcaag aactgaccag gacgcatcac tgttaatttt aataattgat
     1381 gcaggaacat cggaatgtcc gtgagaacaa ccaccgcctg ctattaaagc ataaagatca
     1441 ttgtttataa attctatgtc agctgctcct aacaagtcac ccatagcatt ttgtgaagat
     1501 ggcaaattgt cgactaccgt tgaaatgctg ccgctagaat ttatctttaa aattctagca
     1561 gtatttcctc ccaggtatgg tccgattggt ggaataacct gatcacattg accaagagta
     1621 gaattgtttc cacctagacc ggcttctgca acgtataaat aaccatccgg accaaatttt
     1681 aatccgcgag gagagtttaa tccggaagca aaaacactaa ttgatacatt atctgtttgt
     1741 ttcttttctg ttatttgaga attattaatg ttactatctg tgggtagtac tgcatcatta
     1801 catgaataaa acactaatgt cgataaaagg aaaagaaaca atatttttat tgatttcttt
     1861 ttcatatgcc tgccttttta attttattta ataaatagaa caaagaatta cacaaagaac
     1921 attttgtaaa gtctccaaat atttttaaat gattttacaa cctcctcctt tctttcaaat
     1981 aattaaaaat attttaaaag actttttgga gaaaatattt aagctaaaaa ttatttgcct
     2041 gcaagcagtt ccatcaattt atcaggtgga gttggctgag gcacttcatt atgaatcggt
     2101 ttaatagttc tcaaataagc ataaattgat ctaagatcat catttgtcat ttgacctatt
     2161 gtaggccagg gcattggtgg aagtaaaggt cttcctacac ccatatactt gccttctctt
     2221 agggttttaa taaacatttc ttctgtaaga gctccaattc ccgttgaatt atctggtgta
     2281 agatttgacg catatgaaat accccatggg cctacccagc ccgaaaaatt actttctgtg
     2341 gctacccagt taccgggtgc aacatattta acatccagag ccggaagttt ttcgccttgt
     2401 tgaaaaccgg aaaggcgttt tgttgtatca ggtaccggac cttttgctgt ataaatttta
     2461 ggagtatgac aatcatcaca accacctgtg gtaactaaat atttaccccg agctattaat
     2521 tcctgctggg tcatttgttt ggtctcttct ctgcctgagc aattgaattg tgaaaggatc
     2581 agcaatgata tcattgcaat ggatgctgaa atgaacacac tttttttcat aaggttgcct
     2641 ccttaatatg aaaatatggt taatcgttca gttaatctga tttttgagat ttaagtttaa
     2701 attaattttg cactacaatt aaatcagata aaactggtaa tctgtattgc aaaattaatc
     2761 cttgtaaata aacggtgtta ggaatattca agctaatttt aggactttta ttgcatgttt
     2821 agtttttttc aaattttatt ctaataaaaa tatttttgat tattatttgt aaatactatc
     2881 aactgatatt tttagaaatt agattatgaa tggttagtta tacaagaatt tcaaaaatgg
     2941 ctgattgaga atgataaaaa ttacggatcg attggtgaag taaaaatttt aatgttgtaa
     3001 aattgagaga agagcgagcg acggggatcg agctaatatg tatagattat tattggagtt
     3061 aacgatgatt tataaaatag attatcaatt ctcgttcaca aaaggcaaca aaatgatttt
     3121 tcatattttt aattttgtgt ttaattgtac ttgaagagat gaataatgag attgta
//
LOCUS       DASWBY010000556         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_25937, whole genome shotgun sequence.
ACCESSION   DASWBY010000556 DASWBY010000000
VERSION     DASWBY010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25937"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VF870_10005"
     CDS             <1..529
                     /locus_tag="VF870_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9252566.1"
                     /translation="FIEFGEGDISTKYSALLSKVVRSREGAPIKNPINEPFEGLKKSQ
                     IEEYIDEYFGSGIQHVAITTNDIISTIKAMRANGVEFLSTPPDTYYKMLKEKGWNIKE
                     NIDELQKLGILCDLEGKGYLLQLFTKPVGDRPTFFYEIIQRFEGAEGFGQGNFQALFE
                     SIERDQAVRGNLDKE"
     gene            597..1763
                     /locus_tag="VF870_10010"
     CDS             597..1763
                     /locus_tag="VF870_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX9252567.1"
                     /translation="MPFYVKLGKVPPKRHTTFYKEDGKSLYREELFSTKGFSGIYSNK
                     YHLYIPTKVEMIDEVPIDSSGNDWKDAPLEYYHFITDNKRSRGNLITARNEFLKNEHC
                     VISTAYLSEDTKYFYRNSQMHEMLFVHHGKGVLLTEYGKINFTEWDYLIIPKGTTYQI
                     KFDDYKKNKLFIVESDTPFDIPKHFRNEYGQLTEEAPYYERDFRVPEYTKPVDKLGDF
                     RLLLKVRNRMFEYKLPHHPFDVVGWDGFLHPYAFNIKEYAPKVGKIHLPPPIHLMFVT
                     QHFVVCNFVPRLYDFHPNAIPAPYYHSNVDSDEVLYYVHGDFMSRSGVQEGSITLHPM
                     GIPHGPQPGKTEASIGKKQTDEYAIMIDTFTPLYVTKNVKETMVRDYSQSWLRK"
     gene            1815..2009
                     /locus_tag="VF870_10015"
     CDS             1815..2009
                     /locus_tag="VF870_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252568.1"
                     /translation="MIEENEFTPSVSNEFIVNEPNEFYDDKKDFVTLEAWKKCREVKL
                     FFYNEIIPLIPKEEKYNLGS"
     gene            2010..2270
                     /locus_tag="VF870_10020"
     CDS             2010..2270
                     /locus_tag="VF870_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9252569.1"
                     /translation="MKRASVSATANISEGYGRYHYKESMHFYRISRGSIYELKDHLIS
                     CYDLGYIDEDLKQKAEVMIENAKRTLNGYINFVRGKIQNEKS"
BASE COUNT          921 a          356 c          468 g          776 t
ORIGIN      
        1 ttttatagaa tttggtgaag gcgacatatc aacaaaatat tcggctttac tttctaaagt
       61 tgtaagatct cgcgaaggtg caccgattaa aaatccaatt aatgaaccat ttgaaggatt
      121 aaaaaaatca cagatagaag agtatattga tgaatatttt ggttcaggta ttcagcacgt
      181 agctattacg acaaatgata ttatatctac tataaaagct atgcgtgcaa atggtgttga
      241 gtttttaagc actccgcctg atacttatta caaaatgtta aaagaaaaag gttggaacat
      301 taaagaaaat atcgatgaac ttcagaagct tggaattctt tgcgacttgg agggtaaagg
      361 atatttgctt cagttgttta ctaaaccagt aggagacaga ccaacatttt tctatgaaat
      421 aattcaacgt tttgaaggag ctgaaggatt tggacaagga aactttcaag ctttgtttga
      481 atcgattgaa agagaccagg cagtaagagg taatctggat aaagaatagc aaagttcgag
      541 ttcaggtata agttcaagac agaatcaaga aatagaaata attttaggag tttgaaatgc
      601 cattttatgt taaactagga aaagttccac cgaaacgtca tacaactttt tataaggaag
      661 atggaaaatc tttataccgt gaagaactat ttagtactaa agggttttct ggaatttatt
      721 caaataaata tcacctttat attcctacca aggtggaaat gattgatgag gtcccgattg
      781 attcatccgg aaatgattgg aaagatgcgc ctttggagta ttatcatttc attacggata
      841 acaaacgatc cagaggaaat ttaattacgg cccgaaatga atttttgaag aatgagcatt
      901 gcgttatttc tactgcatat ctttccgaag atacaaaata cttttaccgt aacagccaga
      961 tgcacgaaat gttatttgta catcatggca aaggtgtatt acttaccgaa tatggaaaaa
     1021 ttaattttac tgaatgggat tatctaatca ttcctaaagg aacaacttat caaattaaat
     1081 ttgatgatta caaaaagaac aaactattta tagttgaatc tgatactcct tttgatatac
     1141 caaaacactt tagaaatgaa tacgggcaat taactgaaga agctccatat tacgagcgag
     1201 actttcgcgt tcctgaatat accaaaccgg ttgataaact tggagacttt agactgcttc
     1261 taaaagttcg taatagaatg tttgaataca aactaccaca tcatccgttt gacgttgttg
     1321 gctgggatgg ttttttgcat ccttacgctt ttaatattaa ggaatatgca cccaaggtcg
     1381 ggaagattca tcttccgcct ccaattcatc ttatgtttgt tacacagcat tttgttgtgt
     1441 gtaatttcgt tccgcgtttg tatgattttc atcctaacgc tattccagca ccttattatc
     1501 attcaaatgt tgatagcgat gaggttttat attatgtcca cggtgatttt atgagcagat
     1561 caggtgttca ggaaggatcg ataaccttac atccaatggg aattcctcat ggaccgcaac
     1621 cagggaaaac cgaagcatcg atcggaaaga aacaaaccga tgagtacgcc ataatgatag
     1681 acacatttac tcctttgtat gttactaaaa atgttaagga gacaatggtg agagattata
     1741 gtcagtcgtg gctgcggaag taggcatagg ggaaaaagga ataaggtatc gggaatagga
     1801 tataggtgaa aaaaatgatt gaagaaaacg agtttactcc ttctgtttcg aatgaattta
     1861 ttgtcaatga acccaatgaa ttttacgatg ataaaaaaga ttttgtaact ttagaagcat
     1921 ggaaaaaatg tcgtgaagta aaattattct tttataatga aataatacca cttattccta
     1981 aagaagaaaa atataattta ggtagctaaa ttaagagagc atctgtcagt gcaacagcaa
     2041 atatttctga aggatatggg agatatcatt ataaagagag tatgcatttt tacagaattt
     2101 cgagaggctc aatctatgaa ttaaaagatc atctaattag ttgttatgat ttaggttata
     2161 tcgatgaaga tcttaaacaa aaagctgaag tcatgattga aaatgcaaag agaacattaa
     2221 atggttatat caattttgtt agagggaaaa ttcaaaatga aaagagctag ttcacatttt
     2281 aaaatataat taataaccct attccttatc ccttttaccg tataatataa gcataatttt
     2341 taattataat aaaacaacaa tattggaaaa cattaaatga aattagtatc atataaagaa
     2401 aaaaagacag atagagttgg aatattttat gaaggtaagg tttatgatct tcaaaaatct
     2461 gctatatcgc ttaaaataaa attaccttca acaatgaaag aatttcttga gggtgaaaaa
     2521 a
//
LOCUS       DASWBY010000557        10006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_660471, whole genome shotgun sequence.
ACCESSION   DASWBY010000557 DASWBY010000000
VERSION     DASWBY010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660471"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VF870_10025"
     CDS             <1..253
                     /locus_tag="VF870_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252570.1"
                     /translation="EKYFQLMNLKFSLFVEVNNLLDRQNSAIINPVTGRAYEYGDDLP
                     ASYNDPRYPNLQAPITPYPYNPARYLTRRNIKFGVSLRF"
     gene            255..1232
                     /locus_tag="VF870_10030"
     CDS             255..1232
                     /locus_tag="VF870_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9252571.1"
                     /translation="MKYLITFLLIAISFSEIKPQLFPVLGEQRAGISTAQFLKIGVGG
                     RATAMGDAFIAVANDASALYWNPAGLTQFNENQVMFAHNQWVVDINHDFIGAVYHLDD
                     ENTFGVSLTSVSMDEMKVTTEYAPFGTGEYFGFSDLAIAISYSKKMTDQFSFGGTVRY
                     IEESLDKLKMRGVMIDLGTYYWTGLGSSRFAVTVTNFGNDLAPDGEVVLVGNRTKSDW
                     QSFSPPTMFRIGFALEPYEDDENRITTSIQLNHPNDNSENLSLGVEYVWNQMFFARAG
                     YKINVDEQNYTFGAGVNVPVSIANVSIDYAFANFSKLGSAHRFSITLGL"
     gene            1229..2302
                     /locus_tag="VF870_10035"
     CDS             1229..2302
                     /locus_tag="VF870_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252572.1"
                     /translation="MIKIKHINLLALLTIILGIISCNDKFDITQFDTNDPGNITGDTI
                     YIKVNPDWEGFNHPEEIMVGREPLIYVADTDNDQIVMLNLDGQKLSSRTIKHPIAIAQ
                     DYRLNLIVCAEFDTLGQTFSAVYKIDLVSANHQLENAPIRRLLPQPDDLNQPLRKYTA
                     ACAFYDNSFYISRTGPNNSSIYDPDNSILLFKPISNNFGDGDSLIGRVPNISPTSTGL
                     VSANGISSLASFNKRNIDIVVTLTGMNSFKAQWWNYVVTPIDERYRSAFSPNDGVAFA
                     VPNSFIRPEGCTIDVNGNIFVADAGKDSVYKFNSSGDLMTAFGGPAVFSEPYSVAYFD
                     KILYVADRGNNRIQRFILSTEVQ"
     gene            2299..5571
                     /locus_tag="VF870_10040"
     CDS             2299..5571
                     /locus_tag="VF870_10040"
                     /inference="COORDINATES: protein motif:HMM:NF040146.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX9252573.1"
                     /translation="MKLLFFFFFAFLLTSFLHPQSIIINEIYNSSASDEWVELLVVQD
                     SIDVRGWDLRDFSSSGGAQQPLVFANNQLWSNLRKGTIIIVARSENTFTEDLDASDYL
                     LIVKSTNTLYLTGNPFLIAGSSEAIQIRNNSQTHIFGVSWGTANANSLPSPKVHLAGP
                     SSSGTSVFFNEDNVSKLTNAVNWTMSGAPTMGIGNTAANINWILSLRARVDGSGTVTM
                     TPQIVSGDSTLTLSFNYIRESSYNINTLRIIFPQELTWSQNASQISINNFTASTSVSG
                     DTILFSNIVFLNDSVNINIADITTPYFTGKYKVTFQSGVNLDLGDVSPLPVLTVFGAS
                     IPIAQAKENNSNGIGIHLGDLVTIIGVVTVSDQFGSPSYIQDNSGGISIYGSSFSNAV
                     HIGDEVSVSGIITQFNGLNQLESPILHSIISSGNEVVPLLSTPTMLNNDGVNGIENYE
                     GRLVRVNGVLVKDLNGNSVSNWAYQNYMLTGSSASDTVQIRIDNNTLLIGQVAPAGRF
                     DMVGVLSQYKTTVPFIGGYQLMPRLPEDIIADGPIIEKFPEEVDLTPNSITLAWSTIN
                     PGTSRIRYGTTTNYELGIIETDNDLRTIHNVTVSGLDVATIYNLQAFSVANSDTSFSG
                     NIISSTTSAFPTTGQIDVYFNKNVNTSVSSGVNANGNADFTSILIQKINNAKRSIDVA
                     LYSLSGTVGANIATALVNAKNRGVKIRVIGEYDNRTTAPWNTLISNGIPYINDAYGIN
                     DGSGLNHNKFFIIDYRGGAADSVWVISGSWNPTDPGTNDDRQNLVLIQDVALAGAYTV
                     EFQEEWGSNTDTPNAQNSRFGSRKLNNTPHNFVIGGGVKVQSYFSPSDGTTSKIAATL
                     SKAEKSINGCIMTFTRRDLADTVIAVKNRNRKARLILSNDTDSGTQFSYLQSNGVDIR
                     LKGFTTGLLHHKYAIVDAEPFGYTPYVITGSHNWSSSAENSNDENTLIFQDDQVANFY
                     LQEFAARYYEAGGTDSINTVTSVNEDKSVVPTQFSLLQNYPNPFNPVTTIRFDIPVSQ
                     RVEIVVYDILGRKVKELYNDVAPVGTISIDFRADDLASGMYIYQLKTKEISISKKMVL
                     LK"
     gene            5647..7056
                     /gene="atpD"
                     /locus_tag="VF870_10045"
     CDS             5647..7056
                     /gene="atpD"
                     /locus_tag="VF870_10045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9252574.1"
                     /translation="MSELKGKIIQIIGPVVDIEFEGGNLPKIYNAIRIPRTSTEGKHE
                     DLIVEVQQHLGENRVRAVAMDSTDGLVRGMNVFDSGKPISVPVGPATLGRLINVIGDG
                     IDGLGEIKAEKRYPIHRPAPLFKNLSTQSQMFETGVKVIDLMEPYSKGGKTGLFGGAG
                     VGKTVIIQELINNIAKQHGGYSVFAGVGERTREGNDLWLEMKESGVIDKTALVFGQMN
                     EPPGARLRVALTGLTIAEYFRDEEGRDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGY
                     QPNLATEMGALQERITSTDKGSITSVQAIYVPADDLTDPAPAAAFAHLDATTVLSRQI
                     SELGIYPAVDPLDSTSRILEPGILGQEHYDVAKNCKEILQHYKDLQDIINILGMDELS
                     DEDKIVVRRARRIQRFFSQPFHVAEAFTGKAGKYVKLEDTIRGFKGIIDGKYDDLPES
                     AFMYVGGIEEAVENAKQMA"
     gene            7059..7469
                     /locus_tag="VF870_10050"
     CDS             7059..7469
                     /locus_tag="VF870_10050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX9252575.1"
                     /translation="MAELNLEIVTPSKSAFSGVIKSITVPGTKGRFQVLKNHAPIIST
                     FDLGMIKVDVTDAKADYYATAGGTIEVLDNKVLVLADSIELVTEIDEERALRAKQRAE
                     ERLAEQSSEINLARAQAALARANNRLNVKAKYNQ"
     gene            complement(7526..8149)
                     /locus_tag="VF870_10055"
     CDS             complement(7526..8149)
                     /locus_tag="VF870_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX9252576.1"
                     /translation="MSYTIPIFPLNLVVFPDSKYPLHIFEERYKILLQKCLVENSGFG
                     IVTTIDKRISDVGVYVKITEILKTYLNGEIDIVVQGVERFLINSTSLHPDGYYISDVE
                     KYVDENVIVDQRLNEELQTEFEEIVELANYKLEDTFWNNLKTANNKSYKIAEKSGLTY
                     EQQQELLILKNENERLSYLINYFISIKDKVDRADAVKKIIMNDGYLN"
     gene            complement(8203..8808)
                     /locus_tag="VF870_10060"
     CDS             complement(8203..8808)
                     /locus_tag="VF870_10060"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006822499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX9252577.1"
                     /translation="MAKFELPQLPYAYDALEPYIDKLTMEIHYTKHHNAYVTNLNKAV
                     EGTENAGKSLEEMFSNISKLPVAIRNNGGGHWNHSLFWTLMKQNGGGKPTGKIADAIN
                     SAFGSFDEFKTKFSTAAATRFGSGWAWLLVQDGKLVVSSTPNQDNPLMDVAEVKGIPV
                     LGLDVWEHAYYLKYQNKRPDYIENWWNVINWDKVNELFSKA"
     gene            complement(8938..9288)
                     /gene="erpA"
                     /locus_tag="VF870_10065"
     CDS             complement(8938..9288)
                     /gene="erpA"
                     /locus_tag="VF870_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX9252578.1"
                     /translation="MSNTQIESEITITDKAVKEIRRIMDENKITPEFGLRIGVKGGGC
                     SGLTYTLGFDPETREGDSIIEQEGVKLFVDGKSLFYLSGTQLDFSDGLNGKGFVFNNP
                     NATKSCGCGESFSV"
     gene            complement(9461..9772)
                     /locus_tag="VF870_10070"
     CDS             complement(9461..9772)
                     /locus_tag="VF870_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252579.1"
                     /translation="MSKNISKLFDDLKNESEIFLSYLKAKFPVFHNSNLFSRDFQYGL
                     KSYLEKKGIKITDSNLVNLSKQFSSYYETEGILIKTSDQGWKLNYPEFVTTKPGDPFS
                     F"
     gene            complement(9783..>10006)
                     /locus_tag="VF870_10075"
     CDS             complement(9783..>10006)
                     /locus_tag="VF870_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252580.1"
                     /translation="AFSNSSACSSGSAKPSKILKAIGLTDEQALSSVRFGFGRFNTVD
                     EIDYASQKVIEAVSKIRAIKKINHITIHK"
BASE COUNT         3249 a         1659 c         1815 g         3283 t
ORIGIN      
        1 tgaaaagtat tttcaattaa tgaatctaaa attttctctt tttgttgaag taaataatct
       61 tcttgataga caaaactcgg ccattattaa cccggttact ggcagagcat atgagtatgg
      121 tgacgatttg ccggctagct ataacgatcc gcgatacccg aatttacaag cacctattac
      181 tccttatcca tataatcctg caagatattt aactcgtaga aatataaaat ttggagtgag
      241 cttaagattt taatatgaaa tatttaatta catttttact tatagcaata agtttctcag
      301 aaattaaacc acagttattt cctgtactcg gcgaacaacg tgccggcatc tctacagcac
      361 aattcttaaa aattggtgtt ggcggaagag ctactgcaat gggtgatgct tttatagcag
      421 ttgcaaatga tgcctctgct ctttactgga atcctgctgg tttaacacaa ttcaatgaaa
      481 atcaggtgat gtttgctcac aatcagtggg ttgtagatat caatcatgat tttattggag
      541 ctgtttatca tcttgatgat gaaaatactt ttggtgtttc gttaacttca gtttcaatgg
      601 atgaaatgaa agtaaccaca gagtatgcac cttttggaac gggagaatat tttggattta
      661 gcgatttagc aattgcaatt tcttattcta agaaaatgac ggatcaattt agttttggcg
      721 ggactgtcag atacatcgaa gaatcattag acaaacttaa gatgcggggt gttatgatag
      781 accttggaac ctactattgg acaggattag gaagcagcag gtttgccgta actgtaacaa
      841 actttgggaa cgatcttgcc ccggatgggg aagtagtatt agttggaaac agaacaaaat
      901 cagattggca atcattttca ccaccaacaa tgtttcgtat cggatttgca cttgaaccgt
      961 atgaagatga tgaaaacaga ataaccactt caatacagct taatcatcct aacgataaca
     1021 gtgaaaactt atcattagga gttgaatatg tttggaatca aatgttcttt gcccgtgcag
     1081 gatataaaat aaatgtcgac gaacaaaatt atacctttgg tgctggagta aatgttcctg
     1141 ttagcatcgc aaatgttagt atagattatg cattcgcaaa tttttcaaaa ctcggatcag
     1201 cacatagatt ttcaattaca cttggcttat gattaaaatt aaacacataa atttacttgc
     1261 attacttact attattcttg gtattatttc ttgtaatgat aaatttgata ttactcagtt
     1321 tgataccaat gatcctggca acataaccgg cgatacaatt tatataaaag taaatcctga
     1381 ctgggagggg ttcaatcatc ctgaagaaat aatggttggg agagaacctt taatttatgt
     1441 tgcggataca gataatgatc aaattgtaat gcttaatctt gatggacaaa aattaagttc
     1501 acgaacaata aaacatccaa tcgctattgc tcaggattat agattaaatc ttattgtttg
     1561 cgctgagttt gatacgttag gtcagacttt tagtgcagtt tataaaattg atcttgtttc
     1621 agcaaatcat caattagaaa atgcccctat aagacgttta ttaccgcaac ccgacgatct
     1681 gaatcaacct ttaagaaaat atactgcagc ttgtgctttt tatgataact cgttttacat
     1741 atccagaact ggtcctaaca attcaagtat ttatgatccg gacaattcga tactattatt
     1801 taagcctatc agtaataact tcggtgatgg agattcatta attggaagag tcccaaatat
     1861 ttcaccaaca agcactggtt tggtttcagc taatggaatc agttcattag catcctttaa
     1921 taaaagaaat atagatattg tagttacact gacagggatg aatagtttta aagctcaatg
     1981 gtggaattac gtagtaactc caattgacga aagatataga agtgcatttt caccaaatga
     2041 tggtgttgct tttgctgtcc caaatagttt tatcagacct gaaggctgta caattgatgt
     2101 aaacggaaat atttttgtgg ccgatgcagg caaagatagt gtatataaat ttaattcatc
     2161 tggcgaccta atgaccgcat ttggcggccc agctgtattt tctgaacctt attctgttgc
     2221 atattttgat aagatacttt atgtggctga cagaggtaat aacaggatac aaagattcat
     2281 tttatcaacg gaagtgcaat gaaactatta tttttttttt tcttcgcttt tctattaact
     2341 agctttcttc atccacaatc tataattatt aatgaaatat ataattccag cgcttcagat
     2401 gaatgggttg aattattagt agtacaggat agtatagatg taagaggttg ggacctacgg
     2461 gattttagca gcagtggtgg tgctcagcag ccgctggtat ttgcaaataa tcaattatgg
     2521 agcaatttaa gaaaaggtac aataattatt gttgccagat ctgaaaatac ttttactgaa
     2581 gatctggatg cgagtgatta tttattaata gtaaaatcca ctaatacact ctatctgacc
     2641 ggtaacccat tcttaattgc tggttcatca gaagcaattc agatcagaaa taattctcaa
     2701 acccatatat tcggtgtttc ctggggaact gcaaatgcta atagtctgcc tagtcccaaa
     2761 gtacatttag ctggtccatc gtccagcggc acctcggtat tctttaacga agacaatgta
     2821 tcaaaattaa caaacgcagt taattggact atgagcggtg cgcccacaat gggaatcgga
     2881 aatacagccg caaatattaa ctggatcctt tcattaagag caagagtaga tggttccggg
     2941 actgttacta tgacaccgca gattgtttcc ggagattcca cattgacttt aagttttaat
     3001 tatataagag agtcatctta taacattaat acactaagaa ttatttttcc tcaggaatta
     3061 acctggtctc aaaatgcatc tcagatttca ataaataatt ttacagcatc aacctcagtt
     3121 agcggagata caattttatt ttctaatata gttttcttaa atgactcagt aaatattaat
     3181 atagcagata taactacacc ttactttact ggaaaataca aagtcacttt ccagtccggt
     3241 gttaatttgg atcttggaga tgttagtcct cttcctgtat tgacagtttt cggagcatct
     3301 ataccaattg cgcaggcaaa agaaaataat tcgaatggga tcggaattca tttaggtgat
     3361 ttggttacaa taattggtgt cgtaactgtc agtgatcaat ttggaagccc gagttatatt
     3421 caggataaca gtggtggaat ttcaatttat ggttcaagtt tttcaaatgc tgtccatatt
     3481 ggtgatgaag tttcggtatc gggaatcatt actcaattta atggattaaa tcaactggag
     3541 tcaccaattc ttcattcaat tatcagctct ggaaatgaag ttgtgccttt attatcgact
     3601 ccaaccatgc tgaataatga cggtgttaac ggtattgaaa attatgaagg ccgcttagta
     3661 cgcgttaatg gagttttggt taaagatttg aacggtaata gtgtaagtaa ttgggcatat
     3721 caaaattata tgcttacagg ttcaagtgcc tctgatactg ttcagatcag aattgataat
     3781 aatacacttt tgataggtca ggtagctcct gccggcagat ttgacatggt tggtgtttta
     3841 agtcaatata aaactacggt gccttttata ggtggttatc agctaatgcc caggttaccg
     3901 gaggatatta ttgctgacgg cccaattatc gaaaaatttc ctgaagaagt tgatctgact
     3961 ccaaattcta ttactcttgc ctggagtacc atcaatcccg gaacatcaag aatccgatat
     4021 ggtactacaa ctaattatga acttggtata attgagactg ataatgatct gagaaccatc
     4081 cacaatgtaa ctgttagcgg gcttgatgtt gcaaccattt ataatctaca agcattttct
     4141 gttgctaatt cagatacaag tttttccggt aatattattt caagcactac atcagcattt
     4201 ccaacaaccg gtcagataga tgtttatttt aataaaaacg tgaacacaag tgtatcatct
     4261 ggtgttaatg ctaatggcaa cgcagatttt accagtattc ttatccaaaa aattaacaat
     4321 gctaaacggt caatagatgt agctttgtac agcttaagcg gtacagttgg agctaacatt
     4381 gcaactgcat tagtaaatgc aaaaaatcgt ggtgttaaaa tcagagtaat cggggaatac
     4441 gacaacagaa caacagcacc atggaatact cttattagta atggtatccc ttatataaat
     4501 gatgcatatg gaatcaatga tggaagtggt ttgaatcata ataaattttt tataattgac
     4561 tatcgtggtg gtgcggcaga tagtgtttgg gttatatcag gatcgtggaa tccaactgat
     4621 ccaggtacga atgatgacag acaaaattta gtattaattc aggatgttgc attggctggt
     4681 gcatacacag tcgagttcca ggaagaatgg ggaagtaata cagatactcc aaatgcgcaa
     4741 aattcaagat ttgggtcaag aaaattaaac aacactccgc ataattttgt tattggaggg
     4801 ggagtaaaag ttcaaagtta cttcagcccg agtgatggta ctacttctaa aattgcagca
     4861 actctgagta aagcagaaaa atctatcaat ggttgtataa tgacatttac gagaagagat
     4921 ttagctgata cagttattgc agttaaaaat cgtaacagaa aagcaagatt aattttaagt
     4981 aacgatacag attcaggtac gcagttttca tatttgcaat ccaatggtgt tgatatacgc
     5041 ttaaaaggat ttacaacagg gcttcttcac cataaatatg caattgttga tgcagaacct
     5101 tttggataca caccttatgt aataaccggt tcacataatt ggtcatcatc ggcagaaaat
     5161 agcaatgatg aaaacacttt aatttttcag gatgatcagg tagctaattt ttatctgcag
     5221 gaatttgctg ccagatatta tgaagcaggc ggcactgatt caattaatac tgttacaagt
     5281 gtgaacgagg ataaatctgt agtaccgaca caattttcac tattacaaaa ctaccctaat
     5341 ccatttaacc ccgttactac aattcgattt gatataccag tttctcagag agtcgaaatt
     5401 gttgtttacg atattcttgg cagaaaagta aaagaattat ataatgatgt tgcacctgtt
     5461 ggtacaatat caatcgattt tagggcagat gatttagcaa gtggaatgta tatttaccaa
     5521 ttaaaaacca aagaaatttc tatctctaaa aagatggtac ttttaaagta atttctcaaa
     5581 aatttgttaa aaaaagcctc tttggttatt tttatatttt aaaaatttat tatagaaggt
     5641 aaaaaaatga gtgaattaaa aggaaaaatt attcaaataa taggtcctgt tgtagatata
     5701 gaatttgaag gcggaaatct cccaaagatt tataacgcta taagaattcc tagaacatcg
     5761 accgaaggta agcatgaaga cttgatagta gaagttcagc agcatctcgg tgaaaataga
     5821 gtaagagcgg ttgcaatgga ttctaccgat ggtctggtaa gaggaatgaa tgtttttgat
     5881 tccggtaaac ctatttcagt tccggttggt ccggctacac ttggcaggtt aatcaatgtt
     5941 atcggtgatg gaatagatgg tcttggcgaa atcaaagctg aaaaacgata tccaattcat
     6001 cgtcctgctc ctttatttaa aaatctttca actcaatctc aaatgtttga aaccggtgtt
     6061 aaagtaattg atttaatgga accatatagt aaaggtggaa agactggatt atttggcggt
     6121 gcaggtgttg gtaaaacagt tattattcag gagttaatta ataatatagc taaacagcac
     6181 ggtggttact cagtttttgc aggtgttggt gaaagaacac gtgaaggaaa tgacctttgg
     6241 cttgagatga aagaatcagg tgttattgat aaaacagctt tggtttttgg tcagatgaat
     6301 gaaccaccgg gagcaagact gcgtgttgct cttacaggat tgacaattgc agaatatttt
     6361 agagatgaag aaggaagaga tgttctatta tttattgata acattttccg ttttacacaa
     6421 gccggttcag aagtttctgc actattagga agaatgcctt ctgcggtggg atatcaacca
     6481 aacttagcca ctgaaatggg tgctttacag gaaagaatta cctctacaga caaaggctca
     6541 attacttctg tacaagctat ttacgtccct gcagatgatt taacagatcc tgctcctgct
     6601 gcggcttttg ctcacctgga tgcaactaca gtactaagtc gtcagatttc agagctcggt
     6661 atctatcctg cagttgatcc gttggattca acttcaagaa ttcttgagcc aggcatactt
     6721 ggtcaggagc attatgatgt tgcaaagaat tgcaaagaaa ttcttcagca ttataaagat
     6781 ttacaggata ttattaatat tctcggtatg gatgagttat ctgatgaaga taaaattgtt
     6841 gttagaagag caagaagaat tcagagattt ttcagtcagc catttcacgt tgctgaagcc
     6901 tttacaggta aagcaggtaa gtatgttaag ttagaagata cgattcgtgg atttaaaggt
     6961 attattgatg gcaaatatga tgatttacct gaatcagcat ttatgtacgt tggtggaatt
     7021 gaagaagcag ttgaaaacgc aaagcagatg gcttaataat ggcagaactt aatcttgaaa
     7081 tagtaacacc atccaaatca gcttttagcg gcgtgataaa atcgattaca gtacccggta
     7141 ctaaaggtag attccaggtc ctaaaaaatc acgcacctat aataagtact tttgatcttg
     7201 gaatgattaa agttgatgtt actgatgcaa aagcagatta ttatgcaaca gctggcggaa
     7261 caattgaagt gcttgataat aaagttctgg tgcttgcaga ttcaattgag ttagtcactg
     7321 aaattgatga agaaagagca ttaagagcaa aacaacgagc tgaagaaaga ttggctgaac
     7381 agagcagtga aattaacctt gcaagagcac aagcagcatt agcaagagca aataatagat
     7441 tgaatgttaa agcgaagtac aatcaataaa atttattaag tatagatgtt aaaaaagcga
     7501 gttgatactc gcttttttat tttaattagt ttagataccc gtcattcatt attatttttt
     7561 ttacagcatc agccctgtct actttatctt ttattgatat aaaataatta atcagatagc
     7621 ttaatctttc attctcattt tttaggatta gtaattcctg ctgctgttca taagtcaatc
     7681 ctgatttttc agcgatctta tacgatttat tgtttgcagt ttttaagttg ttccaaaatg
     7741 tatcttctaa tttataatta gctaactcga ctatttcttc aaactcagtt tgtaattctt
     7801 cattaagtcg ttggtcaacg attacatttt catccacata cttttcaaca tctgaaatat
     7861 aatatccgtc cggatgaagg gaagtagaat tgataagaaa tctttcaact ccctgtacta
     7921 caatatcaat ttcaccattt aaataagttt taaggatctc agttatttta acatatacac
     7981 caacatctga tatcctttta tcaatggttg ttacaatccc aaaacctgaa ttttccacta
     8041 gacatttttg cagaaggatt ttgtaacgct cttcaaaaat gtgtaatggg tattttgaat
     8101 caggaaatac aactaaatta agagggaata ttggtatcgt ataactcatg gtataaaaaa
     8161 agggaagtga atttcttcac ttcccatctt gatcattaat aattaagctt ttgaaaataa
     8221 ctcgtttact ttatcccaat taataacatt ccaccagttc tcaatataat ctggtctctt
     8281 gttttgatat ttcaaatagt atgcatgttc ccaaacatct aatccaagaa caggaattcc
     8341 tttcacttct gcaacatcca ttaatggatt atcctgattt ggggtagaac ttacaactaa
     8401 ttttccatcc tgtacaagca accaggccca gcctgagcca aatcttgttg cagcagcagt
     8461 actgaacttc gttttaaatt catcgaaaga accaaatgct gaattaattg catcagcaat
     8521 tttacctgtt ggtttaccac ctccattttg tttcattaaa gtccagaaca atgaatgatt
     8581 ccagtgacct ccgccattat ttctgatagc aacaggtaac ttagaaatat tactgaacat
     8641 ttcttctaat gatttgcctg cattctcagt tccttcaaca gctttgttaa gatttgtaac
     8701 ataagcattg tgatgttttg tatagtgaat ttccattgtc aatttatcaa tgtatggttc
     8761 caatgcatca taagcatagg gtaattgggg caattcaaat tttgccatag tatttgtact
     8821 ccttatttgt ttgttaatat tacttaaatg attttcaact ttgccaagaa cagatactgc
     8881 cggaatggca cctaatgcat aaataaattt tcttcgctgc atcttaatct ctcctttcta
     8941 aacactaaat gattcgccgc aaccacagct ttttgttgcg tttgggttat taaatacaaa
     9001 gcctttaccg tttaatccat ccgaaaaatc taattgggtt ccggatagat agaataaact
     9061 tttaccatct acaaaaagtt ttacgccttc ttgttctata atggaatcac cttcacgggt
     9121 ttcaggatcg aaacctagag tgtaagttaa ccctgaacaa ccgccgcctt taactccaat
     9181 tcttaatcca aattccggtg ttattttgtt ttcatccatt atccgtctta tttctttaac
     9241 ggctttatct gttatggtaa tttctgattc tatttgtgtg ttgctcattg catacctcta
     9301 ttttataatt aacttatttt tattgtaatc taaatttttt aagctctaat gtaaaaacta
     9361 aagtattcct cagttgaaac agtagtaacc gcctcctaaa taacttttaa aaattacaaa
     9421 cttgatcact aaaacttcaa tacaaaaatt attttatatt ctaaaatgaa aaaggatcgc
     9481 cgggttttgt tgtaacaaat tccggataat ttaatttcca cccttgatcg cttgtcttaa
     9541 taagtatacc ttcagtttca taataactag aaaattgctt tgagagatta actagattac
     9601 tatctgtaat tttaatgcct tttttctcaa ggtagctttt caatccgtac tgaaaatctc
     9661 tggagaataa gtttgaatta tggaataccg gaaactttgc tttgaggtaa cttaaaaaaa
     9721 tctcactctc attttttaaa tcatcaaaaa gttttgaaat atttttgctc atgattattc
     9781 aattatttgt gtatagtgat gtgatttatt tttttaatag ctctaatttt gcttaccgct
     9841 tcaattactt tttgagaagc ataatcaatc tcatcaactg tgttgaatct accaaaacca
     9901 aatctgactg aacttaaagc ctgctcatct gttaacccaa ttgctttaag aattttgctc
     9961 ggtttagctg aacctgaaga gcatgcagat gaatttgaga atgcta
//
LOCUS       DASWBY010000558         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_359306, whole genome shotgun sequence.
ACCESSION   DASWBY010000558 DASWBY010000000
VERSION     DASWBY010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359306"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..837
                     /locus_tag="VF870_10080"
     CDS             148..837
                     /locus_tag="VF870_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9252581.1"
                     /translation="MDKKLIIFGANGALGKGVTKTLLKKDYDEIYLFNSKISDSYSDH
                     KIKQFTVKDLNIEKNVIASLFEVKTTDKTKLFLFSTIGGFYGGTSVWETEEVDLDRMI
                     NMNLKTNFFIAKHFSNLVKQSYGGSICLTSAFAGNHPEELKFSYGASKSALNYLVKTL
                     ALEGNSINLSVNAIAPFIIDTEANREWMKNTNYASWMKPEEIGELVHSLFDNYNFITG
                     NIIELKNRFNP"
     gene            986..>1591
                     /locus_tag="VF870_10085"
     CDS             986..>1591
                     /locus_tag="VF870_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9252582.1"
                     /translation="MDFTTTIASIFNILAQATNDGGVMNYLTEKYTAGGIFMHPILAS
                     LIIGLAFSFERLYTLTKARTNTKKFIVDVKKALTSGGVEAAKKVCENTSGPVASVFYA
                     GLLRYDEGLDAAEKAIVAYGGIEMGFLERGLIWISTFITIAPMLGFTGTVQGMIEAFD
                     AIKEAAQISPAVVAEGISVALLTTLFGLVVAMILQVFYNYFI"
BASE COUNT          541 a          240 c          283 g          527 t
ORIGIN      
        1 agtttataaa cccgatatga tggtaacaag aacaggtaaa taccaaggtg cgccgtttcc
       61 aacttatggc ggggcaaaat atcttggtgg ttacagtgat cactatcccg taacagcaaa
      121 attattactg attaaaagat aaacagcatg gataaaaaac tgattatttt tggtgcaaac
      181 ggggcattag gtaaaggcgt aactaaaacc cttttaaaaa aagattatga tgaaatttat
      241 ttattcaatt ccaaaataag cgattcatat tctgatcata aaataaagca atttacagtc
      301 aaggatttaa acatagaaaa aaatgtaatt gcatctttat ttgaagtgaa gacgactgat
      361 aaaacaaaat tgtttttatt ctctaccata ggcgggtttt acggtggaac ttctgtctgg
      421 gaaactgaag aagtagattt agacagaatg ataaatatga atttgaaaac aaatttcttt
      481 atagccaaac atttttcaaa cctggttaaa caaagttatg gtggatcaat ttgtttgacc
      541 tctgcttttg caggcaatca tcccgaagaa ctaaaatttt cttacggtgc ttctaaatcc
      601 gcacttaatt acttagttaa aactttagca ttggaaggga acagtataaa tctatctgtt
      661 aatgcaattg caccttttat aatagataca gaagcaaatc gtgagtggat gaagaataca
      721 aattatgcat cttggatgaa acctgaagaa attggcgaac ttgttcactc tctttttgat
      781 aattataact ttattacggg aaatattatt gaacttaaaa atagatttaa tccttagatt
      841 aactaccgga atcttgtaat aagactaaac tattttgttg actttgtttt ttgaaatact
      901 tattgtagtt acacgttttt aagaaattta tttgatcatt tacaaaattt gaactaaaat
      961 ctaatttacc aggaggttat cttatatgga cttcacaaca acaatcgcat cgatattcaa
     1021 tatcttagct caggctacca atgacggcgg agttatgaat taccttaccg aaaaatacac
     1081 tgcgggtggt atttttatgc atccgatact tgcttcactt ataatcggtt tggctttttc
     1141 atttgaaaga ttatatacat taacaaaagc aagaacaaat actaaaaaat tcattgttga
     1201 tgttaaaaaa gctttaactt caggcggtgt tgaagctgct aaaaaagttt gcgaaaatac
     1261 cagcggacct gttgcttcag tattctatgc tggactttta agatatgatg aaggtttgga
     1321 tgcagcagaa aaagctattg ttgcatatgg cggtattgaa atgggattcc ttgaaagagg
     1381 acttatctgg atttctacat ttattacaat cgcaccaatg cttggattta caggtacagt
     1441 acaaggtatg attgaggctt ttgacgctat taaagaagca gctcagattt ctccggcagt
     1501 tgttgctgaa ggtatttcag ttgccctttt aacaacatta ttcggtcttg tagttgcaat
     1561 gattcttcag gttttctata actatttcat t
//
LOCUS       DASWBY010000559         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_172766, whole genome shotgun sequence.
ACCESSION   DASWBY010000559 DASWBY010000000
VERSION     DASWBY010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172766"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VF870_10090"
     CDS             complement(<1..621)
                     /locus_tag="VF870_10090"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEX9252583.1"
                     /translation="MSNNFLIKIMNKILLHHFQTKQPSIKERIAAGKKLREKFPRINQ
                     GDCKPAANRFDIISILEEQAKARLQELIPIRYSRMLTSPFAFLRGSAAIMAADLATSE
                     TTGINVQVCGDMHVANFGVFASAERNLIFGINDFDETLPGPWEWDLKRLVTSIVVSGR
                     FLGAPENVCKDGVLAAVRSYRKRMKEYAYMGKLKLNYTNIDEKDVLK"
     gene            complement(611..1324)
                     /locus_tag="VF870_10095"
     CDS             complement(611..1324)
                     /locus_tag="VF870_10095"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX9252584.1"
                     /translation="MRTTLIKIASLFSIILIVSHFNLTIAQSDITAIDVLLDPDNTML
                     DSAKSYNLMMQKNYSGPGSYSLDAVHTPHITVLQCFVKTTDLEKVYKAVEKVVNNEKP
                     TEEKLISKGFYYFPYKELGLAGITINTTPKLLRFQEKIIESLKPFIVVGTDAAFVQNA
                     DGTPIAAGSTEYVNGFIQDHAGAKYNPHVTIGLAHENFLKELLAKPYNKFTFKSSSVS
                     IYHLGDFGTARTKLWSNVK"
     gene            complement(1423..2430)
                     /locus_tag="VF870_10100"
     CDS             complement(1423..2430)
                     /locus_tag="VF870_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9252585.1"
                     /translation="MNKIIQYLLGGAAIVIIIAGLKLGADIINQILLSTLIAICIVPF
                     PEWLTRKGLSKGLAITISLLVVLAGGFLTAVLLTNSITGLVESFPVYEQKLTVLYNEF
                     LIYADRNNLKISEIMETVNISPDKILNIAGGVAQSMTNMISSLFVIAMLLVFIVIEMV
                     DFNVDNSTKKSNKALFHNWIGVISLDLRKYISITALKGIITAILNLIFLLIMGVDFAF
                     LWALFSFFMNFIPNLGIIFSFIPPALIALIMLGPWQALTVLIAFWLINFIVENVIGPI
                     FMKESLNISLLNSFLSLLIWGWILGMNGAILGVPLTMVVMKIFKDARNNDNDSLQENT
                     G"
BASE COUNT          897 a          533 c          426 g          947 t
ORIGIN      
        1 ttttaacaca tccttttcat cgatgttagt atagttcaat ttcaatttcc ccatataagc
       61 atattctttc attcgcttac gataggaacg tacggcagcc aatacgccat ctttgcaaac
      121 attttcaggg gctcctaaaa atctaccgct taccactatg cttgtcacca gtcgttttaa
      181 atcccattcc catggaccgg gaagagtttc atcaaagtca ttgattccga aaataagatt
      241 gcgttcagcc gaagcaaata caccgaagtt tgctacgtgc atatcaccac aaacctgcac
      301 atttatgcct gttgtttcac tcgttgccaa gtcagcggcc atgatagctg cacttccgcg
      361 aaggaaagcg aaaggagatg taagcatcct tgaatagcgg ataggtatca actcctgtaa
      421 gcgtgccttt gcctgctctt ccaaaataga gataatatca aagcggttgg cagcgggttt
      481 gcaatctccc tgattgatgc gtggaaattt ttcacgtaat tttttgcctg cggcaatccg
      541 ttctttaata cttggctgct tggtttgaaa atgatgtaag agtattttgt tcataatttt
      601 tattaaaaaa ttatttgaca ttgctccaaa gtttcgttcg agctgtacca aaatcaccga
      661 gatgataaat acttacggat gaacttttaa atgtgaattt gttataaggt tttgcaagca
      721 gctcttttaa aaaattttcg tgggctaatc cgatagtgac atgcggattg tattttgcac
      781 cagcatgatc ttgtataaac ccatttacat attcagtgct tcctgcagcg ataggcgttc
      841 catctgcatt ttgtacaaat gctgcatccg ttccaactac tataaaaggt tttaatgact
      901 caattatttt ttcttgaaat cttagaagtt tcggtgttgt attaatagtt attcctgcta
      961 agcccaattc cttatatgga aaataataga atccttttga tattagtttt tcttcagtag
     1021 gcttttcatt attaacaact ttttctactg ctttatacac tttttccaaa tctgtagttt
     1081 taacaaaaca ttgcaatacg gtaatgtgcg gtgtatgtac tgcatcgagt gaataacttc
     1141 ccggccctga ataattcttc tgcatcatca gattatatga tttggctgaa tccagcattg
     1201 tgttatccgg atcgagcaaa acatcaatag ccgtaatatc tgattgagcg attgtcaagt
     1261 tgaaatgtga gactatcaaa atgatagaaa ataacgaagc gattttaatt aatgtagttc
     1321 tcattttttt aacctttatt acaatttgct aataatttat tatccttaat atttaagtaa
     1381 tttattttat aagtaatttt gtatagcttt accaaaacaa tcttacccgg tattttcttg
     1441 caaggaatca ttatcattat ttctcgcgtc cttaaatatc ttcataacca ccattgttaa
     1501 cggtacaccc agaatagctc cgttcatacc aagaatccat ccccatatca ataatgaaag
     1561 aaatgaatta agaagtgaaa tattgagact ttccttcata aagatggggc cgatcacatt
     1621 ttcaacaata aagttgatca gccaaaaagc aatcagtacc gtgagcgcct gccagggccc
     1681 caacattatc aaagctatta aggctggtgg tataaatgaa aaaataattc caaggttcgg
     1741 aatgaaattc ataaaaaagg agaataatgc ccacaagaag gcaaaatcta cacccattat
     1801 aagcaggaaa attaaattca aaatagcagt gataatacct tttaaagctg ttattgaaat
     1861 atatttccgt aagtctaaac ttataactcc gatccaattg tgaaacaaag ccttgttact
     1921 tttttttgtg ctgttatcaa cgttaaagtc aaccatttct ataacaatga ataccagcaa
     1981 cattgcgatc acaaacaagc tactgatcat attagtcata ctttgtgcaa cgccgcccgc
     2041 aatattcaat attttatcag gacttatgtt tactgtctcc ataatttcag atatcttaag
     2101 gttatttcta tctgcatata ttaaaaattc attataaagc acagttagct tttgttcgta
     2161 aaccggaaaa ctctctacaa gacctgtaat tgaattcgtc agtaaaacag cagttaaaaa
     2221 acctcctgcc aatacaacca gtaatgaaat ggttattgct aatccttttg ataagccttt
     2281 tctggtaagc cattctggaa aaggaacaat acaaatagcg attaatgttg aaagcagaat
     2341 ttggtttata atgtcagcac ctaactttaa tcctgctatg attataacta tcgctgcacc
     2401 accgagtaaa tattgtataa ttttgttcat ctcattttat ccttaacact caccttaaaa
     2461 aaaataattc atatcatatt atttatctta atgcttagtt tgtagcttaa ttacaaaata
     2521 atattgtttg cctgtgctta gaaatatttt tcattctcat ttcatttttc aaagtgtatt
     2581 ttttttccaa tcattattca tatccaggat cacttaacta taatctacag tatcatcaat
     2641 ccttttatag tttctgtaat caaaaacaag ctacttaaat attttgcaaa cattaattga
     2701 ccaagtcttt cgcaaaaaat agatcgctat tggattttgc aaaacttcct atttacaatt
     2761 caaactgggc cctgaattgc cagaaatcag aattaccggt tat
//
LOCUS       DASWBY010000560         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_618885, whole genome shotgun sequence.
ACCESSION   DASWBY010000560 DASWBY010000000
VERSION     DASWBY010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618885"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VF870_10105"
     CDS             <1..1167
                     /locus_tag="VF870_10105"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX9252586.1"
                     /translation="MLEVGMLGVFVSLDLFLFYIFWEAMLIPMYFIIGIWGGKERIYA
                     AVKFFIYTMFGSLLMLVAIVWLAVYAIGPLGYFTTNLVELYKVAPQIPMNIQIWMFLA
                     FAFSFAIKVPLFPLHTWLPDAHVQAPTAGSVILAGVLLKMGTYGLLRFNLPLFPEASI
                     AAAPYISVLAVIGIIYGALVAMVQTDVKKLVAYSSVSHLGFVVLGIFAMTQVSIQGAV
                     IQMINHGLSTGALFLLVGVIYERTHTREIADYGGIAKLVPIFSFALMFASLSSVGLPG
                     LNGFVGEFLILLGSFQSPILNSWWFTVFAASGVIFAAVYLLWMYQRVVFGKVTNPKLD
                     GLKDMNAREIFVLAPIFIFIVWIGIYPTTFLKVSDKSTAKVVKQIYNPVKVTLK"
     gene            1169..2566
                     /locus_tag="VF870_10110"
     CDS             1169..2566
                     /locus_tag="VF870_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX9252587.1"
                     /translation="MKHQKNKYIFFISIIFLLIFFPSEIFASESGEVHSLPNPLMVLP
                     FIILLGTIATGPLFYRHFFEKHYAKISIILGSVTVIYYLAVLKNSTTVVHTLDEYISF
                     IALLGSLFVASGGILINVDRKSTPLLNVFILLFGAVIANVIGTTGASMLLIRPFIKIN
                     KDRIKAYHIVFFIFIVSNIGGALTPIGDPPLFLGFLKGVEFFWVIGHVWYIWLPTILL
                     VTLIFFLIDVRNHPKQESEKEYSGKIEVKGFKNIGFLAIIIISVFLDPAVLSWVPSLD
                     PLPIGIREIIMISIMFLSFKTTDQEILAANEFDFEPIKEVAYLFVGIFATMMPALQLI
                     AQLANENGEQLTPGTFYWATGSLSAFLDNAPTYLTFLSAEMGKFNLNVNLPAEVMSFE
                     NQHPIYLSAISVAAVFFGALTYIGNGPNFMVRSISERSGIKMPSFFGYLFKYAVPVLL
                     PIFTLVWLVFYYGKV"
BASE COUNT          780 a          433 c          491 g          986 t
ORIGIN      
        1 atgcttgaag ttggaatgct cggtgttttt gtttctctgg atttatttct tttctacata
       61 ttctgggaag caatgttgat tccaatgtat tttataattg gaatctgggg cggcaaggaa
      121 agaatttatg cagcggttaa gttttttatt tacacaatgt ttggcagttt gctgatgctt
      181 gttgcaatag tgtggcttgc tgtttatgca attggccccc ttggatattt taccacaaat
      241 ttagttgagc tgtacaaagt tgccccgcaa attccaatga atattcaaat ctggatgttc
      301 ttagcatttg cattcagctt tgcaattaaa gttcctctat ttccattgca cacctggctt
      361 ccagatgcac acgttcaagc tcctactgcg ggttcagtaa tacttgcagg agttttactt
      421 aagatgggaa catacggatt gttaagattt aatctccctc ttttcccgga agcttcaatc
      481 gcagctgctc cgtacatttc tgtgcttgca gtaataggga ttatttacgg tgcacttgtt
      541 gctatggtcc aaacagatgt taaaaaactt gtcgcatatt catcagtatc tcacttaggt
      601 tttgttgtcc taggcatttt tgctatgacg caggtttcaa ttcagggtgc agttattcaa
      661 atgataaatc acggattatc aacaggagca ttattccttt tagtcggtgt catttacgaa
      721 agaacacata cccgagaaat tgcagattat ggtggaattg caaaactcgt tcctatcttt
      781 tcatttgcat tgatgtttgc ttcactttca tccgttggat tgcctggatt aaacggattt
      841 gttggagagt ttttaattct gctcggttct ttccagtctc cgattttaaa tagctggtgg
      901 tttacagttt ttgcagcaag tggagtaatc tttgcagcag tttatctatt atggatgtat
      961 cagcgtgttg tatttggaaa agttactaat ccaaaattag atggactaaa ggatatgaat
     1021 gcaagagaaa tatttgttct tgcaccaatc tttattttca ttgtatggat tggaatttat
     1081 ccaactacat tcttaaaagt atcagacaaa tcaactgcta aagtagtaaa gcaaatttat
     1141 aatcctgtaa aagtgacttt gaaataaatt gaaacatcaa aaaaacaaat acattttttt
     1201 tatttcaata atctttctac ttattttttt cccatcagaa atatttgctt ctgaatccgg
     1261 cgaagttcat tcattaccaa atccattgat ggttttacca tttattatcc tgttgggaac
     1321 tatagcaact ggaccgttat tttaccgtca tttttttgaa aaacattatg caaagatttc
     1381 aatcattctg ggttcggtga ctgtcattta ctatctggca gtcttaaaaa attctacaac
     1441 cgtcgttcac acactggatg agtatatctc ttttatagct ttacttggat cactgtttgt
     1501 tgcctcaggt ggaatattaa tcaatgttga tagaaaatcg acaccattgt taaatgtttt
     1561 tatattactt tttggggcgg taatagcaaa tgtaattgga acaacgggtg catcgatgtt
     1621 attgataaga ccatttataa aaatcaataa agatcgtatc aaagcttacc acatagtttt
     1681 ttttattttt atcgtcagca atattggcgg cgcacttact ccaatcggag atccaccatt
     1741 gtttctggga tttcttaaag gggtagaatt cttttgggta atagggcatg tttggtatat
     1801 atggcttcca acaattcttt tagttacact gatatttttt ttaatagatg ttagaaatca
     1861 tccaaagcag gaatcagaaa aagaatactc tggaaaaata gaagttaaag gctttaagaa
     1921 catagggttt cttgcaataa taataatttc tgtgtttctt gatccagcag tattaagctg
     1981 ggtcccttca ttagatcctt taccaattgg aataagagaa attattatga tatcaataat
     2041 gtttctttct tttaagacta cagatcaaga aattttagca gcaaatgaat ttgattttga
     2101 acccatcaaa gaagttgcct atttatttgt aggaattttt gcaactatga tgcctgcttt
     2161 acaattaatt gcacagttgg caaatgaaaa tggagagcag cttacaccag gtacttttta
     2221 ttgggctaca ggatcactct ctgcattctt ggataatgct ccaacatatc ttacattctt
     2281 aagtgctgag atgggaaaat ttaatttgaa tgttaattta ccagctgagg tgatgtcatt
     2341 tgaaaatcag catccaattt atttatctgc gatttctgtt gctgcggtat tttttggagc
     2401 cttaacttat ataggcaatg gaccaaactt tatggtaaga tctatcagtg agcggtcagg
     2461 tataaagatg ccaagttttt ttggttatct attcaaatat gctgtccctg ttctgcttcc
     2521 tatttttact ttagtttggt tagtgtttta ttatggaaag gtgtaaaatg aaaactttta
     2581 aagatgcatt aaataaaaat ttaattggta tttattatgg taaccgggtt ttacttccgt
     2641 tccattgcca tatattaaag atcgttattg aaaataatat aataaccgat
//
LOCUS       DASWBY010000561         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_284524, whole genome shotgun sequence.
ACCESSION   DASWBY010000561 DASWBY010000000
VERSION     DASWBY010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284524"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="VF870_10115"
     CDS             complement(<1..1457)
                     /locus_tag="VF870_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX9252588.1"
                     /translation="MTKLDSKIPEGNISEKWTNHKFNMKLVNPANKRKYDIIVVGTGL
                     AGASAAASLGELGYNVKAFTFHDSARRAHSIAAQGGINASKNYQNDGDSTYRLFYDTV
                     KGGDFRAREANVYRLAEVSGNIIDQCVAQGVPFARDYGGLLDNRSFGGAQVSRTFYAR
                     GQTGQQLLLGAVSALNRQVDKGSVKLYLRREMLDVVIVDGAAKGIVVRNLLTGEIEKY
                     SAHAVVLATGGYSNVFFLSTNAMNCNATAIWRAHKRGAYFANPCFTQIHPTCLPLHGE
                     SQSKLTLMSESLRNDGRVWVSKKKADMRKPEDIPEDERDYYLERKYPSFGNLAPRDIS
                     SRAAKEVCDEGRGVKGGEAVYLDFKDAINRLGQKVIEERYGNLFEIYETITGDNPYKL
                     PMMIYPAPHYTMGGLWVDYNLMSNIPGLFVLGEANFSDHGANRLGASALMQGLADGYF
                     VIPYTMGNYLAGDKPTLVKTDHPEFEKVAKEVKDQT"
     gene            complement(1457..2128)
                     /locus_tag="VF870_10120"
     CDS             complement(1457..2128)
                     /locus_tag="VF870_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEX9252589.1"
                     /translation="MGWFFKFINSSIGKKFMMAVTGSFLLIFLIIHLIGNITLFFGPG
                     AFNAYVSALDTIKPLIRVIEVVLLAAFVLHIFNGLRLWIENKVARGTNYKVNGSSENS
                     TVFSRTMFLTGSIVFIFLVLHLGTFFWRFNVHDPEGLAKHHQYYDIVLGFLQMPWYSI
                     LYIIAVLLLGFHLNHGFQSAFQTFGWNHKRYFPLIQKIGTLYAIIMALGFASMPLYFL
                     LGGGN"
     regulatory      2383..2684
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          926 a          543 c          473 g          875 t
ORIGIN      
        1 gtttgatctt ttacttcctt cgcaactttt tcaaattcag gatgatcggt ttttactaaa
       61 gtcggtttat cgccggcaag ataatttccc attgtgtaag gaataacaaa atatccatca
      121 gctaatcctt gcataagtgc agaagcacct aaacggtttg ctccgtgatc agagaagtta
      181 gcttcgccaa gtacaaacaa ccccggaatg ttgctcatca aattgtaatc tacccataag
      241 ccgcccatgg tataatgcgg agccgggtaa atcatcattg gcaatttgta aggattatct
      301 cctgtaatag tttcataaat ttcgaacaag tttccgtaac gttcttctat cactttttgt
      361 ccaaggcgat taattgcatc tttgaaatca agataaaccg cctctccgcc tttcacacct
      421 cttccttcat cgcagacttc ttttgctgca cgtgatgaaa tatctcttgg tgcaagattt
      481 ccgaatgaag gatattttct ttcgagataa taatctcttt catcttcagg aatatcttca
      541 ggttttcgca tatcagcttt tttcttggaa acccaaactc ttccatcgtt acgcaaagat
      601 tcactcatca aggttaattt tgattgtgat tcaccatgca atggtaaaca agttggatga
      661 atttgtgtaa agcagggatt tgcaaaataa gctccgcgtt tatgcgcacg ccagattgct
      721 gttgcattac agttcattgc atttgtggac aagaagaaaa cgtttgagta cccgccggtt
      781 gctagtacaa ctgcgtgagc agaatatttc tcgatctcac cggtaagtaa atttctaacc
      841 acaattccct ttgctgcacc atcaacaatt acaacatcaa gcatctctct tcttaaatat
      901 agcttaactg aacctttatc aacctgtctg tttaatgcac ttacagcacc gagcaataat
      961 tgctgccccg tttgtcctct tgcataaaaa gttctggaaa cctgtgcacc gccaaatgaa
     1021 cggttatcaa gcaatccgcc gtaatctctt gcaaacggaa caccttgtgc aacacattga
     1081 tcaataatat taccgcttac ttctgcaaga cgatatacgt ttgcttcccg tgcacggaaa
     1141 tcgccgcctt tcacagtatc ataaaataat ctataagttg agtcaccgtc attctgataa
     1201 ttcttggaag catttattcc gccttgtgcc gcaatactat gtgctcttct tgcactatca
     1261 tgaaaagtaa atgctttaac attataacca agttcaccaa gagaggcagc cgcagaagca
     1321 ccagctaatc cggttccaac aacaataata tcatattttc ttttgttagc aggattaacc
     1381 aatttcatgt tgaatttgtg gttagtccat ttttcagaaa tatttccttc gggaattttt
     1441 gagtcaagct ttgtcatcag ttacctcctc ctaataaaaa gtaaagtggc attgaggcaa
     1501 accctaaagc cataataatt gcgtataagg ttccaatttt ttgaataagc gggaaatatc
     1561 ttttgtgatt ccatccaaaa gtttggaatg cactctggaa accatgatta agatgaaaac
     1621 ccaaaagcag tactgcaatt atataaagga tcgagtacca gggcatttgc aaaaatccca
     1681 atacgatatc ataatattgg tgatgctttg ctaatccctc aggatcatga acattgaatc
     1741 tccagaaaaa tgttccaaga tgcagaacaa gaaaaatgaa aacaattgaa ccagttaaaa
     1801 acattgttct ggaaaatact gtactgtttt ctgacgaacc atttactttg tagtttgttc
     1861 cacgtgctac tttgttttca atccataatc ttaatccgtt aaagatatga agaacaaaag
     1921 ctgcaagtaa aactacttca ataactctga tcaaaggttt gattgtatca agtgctgaca
     1981 cgtatgcgtt aaaggccccg ggaccaaaaa acaaagtaat atttccaatc aaatgaatga
     2041 ttagaaaaat tagcaagaaa ctccccgtaa cggccatcat aaattttttg ccgatggagg
     2101 aattgatgaa tttaaaaaac cagcccatta aaagctcctt ttagatgaag attgatttat
     2161 agctgatgtg aaattaaaga ttatattttg tgataagtgt aaataaccag gtactacgaa
     2221 tattaagatt ttaaccggaa tcatctgtga ttatataaaa ttggtaatta acaataaatc
     2281 tcacatgcaa cttagaaaaa aaagttaaag gtagaaagta attcaataaa aaattttaaa
     2341 gttatttgag aaagaaatta ataagactat tttagactta gttctttgaa caaaatttac
     2401 ggtgttccaa ccccgacaag tcggagtaat ggaaaattat agggaagtac ggtgagataa
     2461 tttatcgtca ttacaagtgc tgaagggacg aagtaatctg tttaattgat agatttcttc
     2521 aattcgttag taatgactaa aagaatcaat tctgtcgctg tcgcgcaact gtgaatcaat
     2581 ttacaatgta aaatgcataa tgtacaatta aaacaattgt acattgttaa ttgataattg
     2641 tacattgaaa agccaggaga cctgccgcaa atactaatta atcaaccttc gcgagaaagg
     2701 taaggttaac gatggtcttt tataatctcc attcgtttta tctaaacttt taatacgcga
     2761 agttactttt aacctttttt aaggagtaac ttcaatgcac aaaacaaaat ttctttt
//
LOCUS       DASWBY010000562        12352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_208013, whole genome shotgun sequence.
ACCESSION   DASWBY010000562 DASWBY010000000
VERSION     DASWBY010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 12352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12352
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208013"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VF870_10125"
     CDS             complement(<1..446)
                     /locus_tag="VF870_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252590.1"
                     /translation="MKKKLKSKPKLLIIVLFQNLLDEEPYYAKNPAPPLPGILLAALT
                     PPEVEVEVLHEMVRPIDYNTDADFIAISFMDYLSPHAYEVAAKFRAMGKIVIGGGKFA
                     STFPEEVEPYFDSICIGEAHKVWAEMVNDLLSGKLKKRYDAGLAPD"
     gene            complement(475..1782)
                     /locus_tag="VF870_10130"
     CDS             complement(475..1782)
                     /locus_tag="VF870_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9252591.1"
                     /translation="MSAKQEYLGGENFDLRDILLRGRRMPLTDRVNFFGDFLENLSSN
                     KQMLHLRCISSPSDREVDVYDEYLGITKKMLMFGSNNYLGFANHPYIKEYVKDIIEKY
                     GIGIGGPPLLNGYTKIHRELEERLASLKGAEDALIFSSGYGANVGLVSALVGPDDYVV
                     YDQYSHASFYDGLKMCNARGIHFLHNDVENLKAKLEDARKLNPRNVYVGVEGVYSMDG
                     DIAPLDEIVEVCKQYNAILLVDDAHGTGTIGQHGGGSGDYFGLDGEIDVVMGTFSKSF
                     AVTGGFVAAEKPIVDYLRFFARSYMFSASISPITIASVLAGLDLLENDPSILNALRSN
                     VKYAAKLLNRIGFNVASETPIIPLRVPMEMNIRDAAYKFHERGIFINSIEYPAVPVSQ
                     QRFRISITASHTKEDINKLVAVVEEIWTDYESNRSETDILRAM"
     gene            complement(2019..3125)
                     /locus_tag="VF870_10135"
     CDS             complement(2019..3125)
                     /locus_tag="VF870_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX9252592.1"
                     /translation="MHPLKDIIENKIIKDLTKSFSRSPFQLNKVHETDAEIIRLENST
                     YDLAVTIDTISEEIKTGLYSDPYQIGWLSVMVNMSDLAAVGASPLGIVISQILPKEIP
                     EEYLNEIQKGINDACIKCGAFVLGGDTNFGDELIISGCAIGTVEKGKYLKRVGCKADD
                     KIYITGKAGFGNAYAAQKLLLNNSELSFRTGLRERTPNEDKGENDNTRFFPEAKLEWS
                     NILKKYSSSCIDTSDGIISSLDQLMRLNNVGFKFRNDWVESIDDESKRLFINFKLAPW
                     LLLAGEHGEFELLFSIPADRKIDFLKEAHLSNLKPICLATATEYENIKINLYDKEQIL
                     DTENIRNLAFEKDFTPAKYLQKLMEIDHKMSNLF"
     gene            complement(3113..4045)
                     /locus_tag="VF870_10140"
     CDS             complement(3113..4045)
                     /locus_tag="VF870_10140"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9252593.1"
                     /translation="MYLLLNTYAAGGSANRKWDQIKNVIQKKFNELKLITLNNYIEAN
                     RKIKDLLKGEEKDFIIAGGDGTVNFFINNLIDSTNAQEIKKFRIGAIGIGSSNDFHKP
                     IKKENLINKIPAKINFNDFQPRDVGVIKYRSGNQILHKYFLLNASIGITAAANQLFNK
                     PDLILKFLKKYFTPIAIVYAALKTILTYKNFEGEIIFSPYEAYSFRISNLSILKNPNI
                     SGDLSYPGKADYQNGLYDIYLTHSMNKLDLINLLKSLSKQTFPKNEKTKYCKTSKLKV
                     TAEKDFLIEFDGETISTNNAEFSILTKYLNICTH"
     gene            complement(4048..5037)
                     /locus_tag="VF870_10145"
     CDS             complement(4048..5037)
                     /locus_tag="VF870_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX9252594.1"
                     /translation="MEPLTRSKYHKTFSIDFVKDYIITMRPYLLFVSGVTGICGMSFI
                     SELSITKTILIFLASFLSYGFGQALTDCFQTDTDSLSSPYRPLTQGRISKTQVLSISI
                     AGLIFCISVFAIYNPVNLLLGVVSGFGLATYTYFKRKFWGGPFYNAWIVGVLFIMAYL
                     CGSDLETLIRNHKLVYGLSAVFWGYANFVLVGYFKDVEADRATGYNTFLVVFGRRMSS
                     FASNIFGVLTILSAISVFTYSSPFDHLFTGRIIETIILFIGILVMCLCQVLVHLIKHD
                     DESSNAISLSVHAYILLLISIIVLNKNEWLIPMIIFYVLFNLTMIFRPAKNQI"
     gene            5257..5448
                     /locus_tag="VF870_10150"
     CDS             5257..5448
                     /locus_tag="VF870_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252595.1"
                     /translation="MKSLLAFALIQIMIYIFSMSNAITIGLIVLGSAIILFAGAYLSA
                     LVKDKKHRRKNISAALELQ"
     gene            5624..5833
                     /locus_tag="VF870_10155"
     CDS             5624..5833
                     /locus_tag="VF870_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9252596.1"
                     /translation="MKELFSVQEVAKLLDMSHSGVLYHIKNGSLKSTQVGKIYIISKE
                     DFADFLKEQKKEKKSKKKPEEPLLF"
     gene            complement(5834..6274)
                     /locus_tag="VF870_10160"
     CDS             complement(5834..6274)
                     /locus_tag="VF870_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252597.1"
                     /translation="MKYHSIIFFLLCTNILLTAQDKRYTKGAENGYAWLTLNQSNNIL
                     TDYKFEYLASMLENQRYMIKYENKPQMPLGCIDDVAKLGESGKSEELDLNFMVEMIDE
                     FYSRKENLIIPIIGAYCYCIKELTGISIKDLEKYRQELLAFSKE"
     gene            6362..7855
                     /locus_tag="VF870_10165"
     CDS             6362..7855
                     /locus_tag="VF870_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEX9252598.1"
                     /translation="MQIGFLMGSHLLSKSSFIRGVQCEKHLYLYKYHYKEMDQLSDMQ
                     KAIFKRGTDVGKLAQQLFPDGIDASPATQFEYNKAVIATKNLIDKKQKVIYEASFNHS
                     DVLAVADIILNEKSGLKVFEVKSSTSISETYIRDAALQYWVISNCGYKIKDFSITYIN
                     NQYIRKGELELDKLFITESVLKLILPLQKWVEENVNRFKQVLAKKAIPKIDIGEHCYD
                     PYTCGFYEYCRNHIPENSIFDLSGVHLNKKYDFYRSGIIKLKDIPVEVELPKNAKIQL
                     DIFKSKIDLIDKKAIKEFLCDLNYPLYFMDFETFQAAVPIFNNSKPYMQIPFQYSLHY
                     KKNKKSKIDHYKFLAETGIDPRIKFIENLLRDTKSEGDILTYNKSFEVLRLKEIAEAF
                     PKYEKEIEERISRIKDLMLPFQKKYYYTHQMHGSYSIKYVLPALIPELSYENLEINEG
                     GLASIAFESLYYETDLMRIADIRKNLLEYCKMDTFAMVKLLEKLESL"
     gene            7867..8709
                     /locus_tag="VF870_10170"
     CDS             7867..8709
                     /locus_tag="VF870_10170"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX9252599.1"
                     /translation="MKKVLFPLLFIFITLSTFAQTVDIKVMTYNIRCGFCEDSSDVNN
                     WSKRKYLVAYLIKNHNPDLIGLQEAEMFQVKELIEMLDEYDWYGVSREDGKEGGESTA
                     ILYKTKRFKLEEKYTIWLSETPELVSKGWDAAFKRTVTIAKLKDLQSKKEFYYLNTHF
                     DHIGETARTESSRLIVKEINKYSKDYPIILSGDFNYTSSSDGYKIITGKLFDSKSVSK
                     KESLGGNITFNGFGKDIQPDNKIDFIFVNNKIEVLNHLIDTTTFNGLYPSDHYPVIAD
                     LLLK"
     gene            8895..10124
                     /locus_tag="VF870_10175"
     CDS             8895..10124
                     /locus_tag="VF870_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252600.1"
                     /translation="MKISISFLYIIIFFSSQIFAQWNKVEDITSPFVYSVLFDADNIF
                     VGGDSLYISRDRGLTWQSATLIGQVIEITALFKYDNMIFAGTYGSGVFISSNNGSSWQ
                     SYNSGLGAFALYAKKFVSSGDTIFYATDGGGVYYLLNNTNVWQSYNQNLPSSIAWTIN
                     DIAVTNNNIIVSAGGSGFYYLRPKGSAEWIEKRMQTPQGIFMTPNAFLSTGNIVFSGH
                     RFGIYKSLDEGNTFDSVGISAMDIAVVSFVMDENRIYAGYTRSSGNDFFVWYSDDLGQ
                     SWNIFAHEFQFLLNLYIYDNKIWAGTNDGLYYTQLQPSNVEPIDIPHKLKLDQNYPNP
                     FNPTTKISWQSSISGRHTLKIYDVLGNLVETLVDEDRPAGNYNITFDASSLSSGVYFY
                     KLQIGSYIETKKMILLM"
     gene            10196..10891
                     /locus_tag="VF870_10180"
     CDS             10196..10891
                     /locus_tag="VF870_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9252601.1"
                     /translation="MNLADGILWYIVFLFSTVFHEAAHAFTAHKMGDSTAHKSGQVSL
                     NPIPHIKREPIGTVIVPVLSFLAGGWMIGWASTPYNVQWAHDYPKKAAAMAAAGPISN
                     FILIIISAVIIHVGISSGFFYQPDSITISGIVSGANSGWLEILAKIVSVMFSLNLILF
                     VFNLLPFPPLDGSAIPPMYLSEDAGRKYIAFIRKPVFALAGLFVAWNLLDIIFNKIHL
                     VVINLLYPGSNYH"
     gene            10938..11642
                     /locus_tag="VF870_10185"
     CDS             10938..11642
                     /locus_tag="VF870_10185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9252602.1"
                     /translation="MNIKDSTFLVTGGSAGIGKATAKLLVEKGGKIAITGRDKSKLEK
                     VAKEIGAFAIHADVAVGSDVHKTYELFLKQFGKLDCLINNAGIGDAWNEITELDMKAF
                     QRVYDVNVFGAALMASEAAKLFKKQKSGNIINIASTAGTKGFANGTVYASSKFALRGM
                     TQCWQAELRKYNVRVILVNPSEVTTAFGDNERNEKSEVKNKLRPLEIAHTIVTALEMD
                     DRGFIPEVTVWATNPF"
     gene            11670..12302
                     /locus_tag="VF870_10190"
     CDS             11670..12302
                     /locus_tag="VF870_10190"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9252603.1"
                     /translation="MKNNSETFNWLEVAREIQQLSQTGLAFAVTDYEKNRYKRLTEIT
                     AEIIEHHTTLEKESVTKILMKHPGYATPKIDVRAAVIKDKKILLVQETTDNCWAMPGG
                     WADVGDIPSEVAIRECKEESGYDVKPIKVIGVFDANRIGGHLEFFHAFKIIFLCELIG
                     GEARTSDETIDVQFFDFDKLPALSLNRTNDKHLNEIKQHLIDPKRKTFFD"
BASE COUNT         4097 a         2044 c         2061 g         4150 t
ORIGIN      
        1 aaatcggggg ctaatccggc atcatatctt tttttcaatt tacctgataa caaatcatta
       61 accatttcag cccaaacttt atgagcctcg ccgatacaaa ttgaatcaaa atatggttca
      121 acttcttccg gaaatgtgga agcaaacttt ccacctccaa ttacaatttt tcccattgcc
      181 ctaaactttg cagcaacctc ataagcgtga ggtgatagat aatccataaa acttatagca
      241 ataaaatcgg catctgtgtt gtaatcaata ggtcgaacca tttcatgaag aacttcaacc
      301 tcaacttcgg gtggagttaa agcggcaagt aaaattcccg gtaatggcgg ggcaggattt
      361 ttcgcatagt acggttcttc atccaagaga ttctgaaata aaactattat taaaagtttt
      421 ggtttagatt ttaatttctt tttcatttta taattcatcc tttctttacc ttagttacat
      481 tgcccttaaa atatcagttt cggagcggtt actttcgtaa tctgtccaga tttcttcaac
      541 tactgctaca agtttgttta tatcttcttt tgtgtggcta gcagttatgc tgatgcggaa
      601 tctttgttgt gaaaccggta ctgcaggata ttcaatcgaa tttataaaaa ttcctctttc
      661 gtgaaattta tatgcagcat ctcgaatgtt catttccatc ggcactctta atggaataat
      721 tggtgtttca cttgcaacat taaaaccgat tctatttaat agctttgcag catattttac
      781 attgcttctt aatgcgttta agattgatgg gtcattttca agtaaatcta atccagctaa
      841 aacagaagct attgtaatcg gagagatcga agcagaaaac atatatgatc ttgcaaaaaa
      901 tcttaagtaa tctacgattg gtttttcagc agcaacaaat ccgccggtta cagcaaatga
      961 tttactaaac gtacccatta caacatcgat ctcgccatcc aatccgaaat aatcaccgga
     1021 gcctccgccg tgctgcccga tagttcctgt tccatgagca tcatcaacta gtaaaattgc
     1081 attgtattgt ttacagactt ctactatctc atcaagtggc gctatatctc catccataga
     1141 ataaactcct tcaactccta cataaacatt tcttggatta agttttctcg catcttccaa
     1201 ttttgctttt aagttctcca catcattgtg aaggaaatga atcccacgtg cattacacat
     1261 tttcaatcca tcataaaatg atgcgtggct gtattgatcg taaacgacat aatcatccgg
     1321 cccaactaaa gctgaaacta atccaacatt cgcaccatat ccactggaaa aaattaatgc
     1381 atcttcagct cccttaagag aagctaatct ttcttccaac tccctatgga tttttgtgta
     1441 tccgtttagt aaaggaggtc ctccaattcc aattccatat ttttcaatta tatcttttac
     1501 atactctttt atgtatggat gatttgcgaa tcctaaataa ttgtttgatc cgaacattaa
     1561 catttttttt gtgataccta aatattcgtc ataaacatca acttcacgat cagatggact
     1621 tgaaatgcat cttaaatgaa gcatttgttt gttgctggat agattttcaa gaaaatctcc
     1681 aaagaaattt actctatccg ttagaggcat tcttcttcct cttaagagaa tatctctaag
     1741 atcgaaattt tctccgccta aatattcttg ctttgctgac attttatttg ctcactttct
     1801 cgtcttgtga tacttacctg atttctgata gaaaactaat atgattttaa gagggaagtg
     1861 tcagtaagtt ttaatagaat tgtcaaaaat ttgtcatgaa ttgtatgatt gatggataag
     1921 tttatttttt tgataaaaac aatttaccgt ctgagcgaag tttaaggcac ttttaatgaa
     1981 attttccaca cctcgactcc gctcggtgtg acggcgcttt aaaaaagatt actcatcttg
     2041 tgatcaatct ccatcaattt ttgcagatat tttgctggcg taaaatcttt ttcaaaagca
     2101 agatttctaa tattttctgt atctagtatt tgttccttat cataaagatt gattttaata
     2161 ttttcatatt cagtagcggt ggccaggcaa attggtttca ggtttgataa atgagcttct
     2221 ttgaggaaat caatttttct atcagccgga atggagaata ataactcaaa ctcgccatgt
     2281 tcgccagcca gcagaagcca gggagctaac ttaaaattaa taaataatct ttttgattca
     2341 tcatcaattg attcaaccca gtcattcctg aatttaaacc ccacattgtt taatctcatt
     2401 aactgatcaa gtgaagaaat tattccatca ctagtatcaa tgcagcttga agaatatttc
     2461 ttcagaatat tactccattc taattttgct tccggaaaaa atctagtatt atcattctca
     2521 cctttatctt cattcggagt tctctctcgt aaacctgttc gaaatgacaa ttcggaatta
     2581 ttgagcaata acttttgggc tgcataagca ttgccaaatc cagcttttcc tgtaatataa
     2641 attttatcat cagctttgca tccaactctt tttaaatact ttcctttttc aactgttcca
     2701 atagcacaac ctgaaataat taattcatct ccaaaattag tatcaccgcc aagaacaaat
     2761 gctccgcatt ttatgcaggc atcatttatt cctttttgaa tttcgtttaa atattcttca
     2821 ggaatttctt taggaagaat ttgcgaaata actattccta aaggagaagc acctacagca
     2881 gcaagatcac tcatattgac cattacactc agccacccga tttgatatgg atctgaatac
     2941 aatccggttt ttatttcttc ggaaattgta tctatagtga cagctaaatc ataagttgag
     3001 ttttcaagtc gaataatctc agcatctgtc tcgtgaacct tgttaagctg aaaaggcgaa
     3061 cgtgaaaatg atttcgtaag atcttttata atcttatttt caattatatc ttttaatggg
     3121 tgcatatgtt taagtactta gttagtattg aaaactcggc attatttgta gagatagttt
     3181 ctccatcaaa ctcaattaga aaatcttttt ctgctgttac ttttagtttt gatgttttac
     3241 aatattttgt cttttcgttt ttaggaaaag tctgcttact taaagacttt aatagattta
     3301 ttagatctaa tttgttcata gagtgagtta gataaatatc atataatccg ttttgataat
     3361 ccgctttgcc tggatatgaa agatctcccg atatatttgg atttttaagt attgataaat
     3421 tagaaatccg aaaagaatat gcttcatagg gactgaagat aatttcgcct tcaaaatttt
     3481 tataagtaag aatagttttt aatgcagcat aaacaatagc aatcggtgta aaatattttt
     3541 ttaggaattt taatattaaa tccggtttat taaaaagctg atttgctgca gcagtgatac
     3601 ctatactggc atttagaaga aaatatttat gtaagatttg attgccggat ctgtatttta
     3661 ttactccgac atctcttggc tgaaaatcat taaaatttat tttagccggt attttattaa
     3721 tcaggttttc tttttttatc ggcttatgaa aatcattact cgatccgatt ccaatagcgc
     3781 caattctaaa tttctttatc tcctgagcat tagttgaatc aattaaattg ttaatgaaga
     3841 agttgactgt tccatctccg cctgcaataa taaaatcttt ttcttcaccc ttaagcagat
     3901 ccttaatttt tctatttgcc tctatataat tattaagcgt aattagtttt aattcattaa
     3961 actttttttg gatgacattt tttatttgat cccattttct attggcactg ccgccggcag
     4021 cgtaagtatt taataaaaga tacattttta tatttgattt ttagctggtc tgaatatcat
     4081 tgttaagtta aatagcacat aaaaaataat cattggaatt agccattcat ttttgttcaa
     4141 aactattatc gatataagta ataaaatata ggcatggact gataagctaa tagcgttgct
     4201 ggattcatca tcatgtttta ttaaatgcac taatacctgg cataaacaca taactaaaat
     4261 tccaataaaa agaattattg tttcaataat ccttccggta aaaagatgat caaatggact
     4321 tgagtatgtg aaaactgaaa ttgcagacag gattgttaac actccaaaaa tattagaagc
     4381 aaatgatgac attcttcttc cgaatacaac cagaaaagta ttgtaacctg ttgctctatc
     4441 tgcttcaaca tctttaaagt atccgacaag tacaaagttc gcatatcccc agaagacggc
     4501 acttaatcca taaaccaatt tgtgatttct aatcagcgtt tctaaatctg aaccacagag
     4561 gtaagccata ataaataaaa caccaactat ccatgcattg taaaatggtc ctccccagaa
     4621 ttttctttta aagtaagtgt aagttgctaa tccaaatccc gatacaaccc ctaataaaag
     4681 gtttacagga ttgtaaatgg caaatacact tatacagaaa atcaatcctg caatgcttat
     4741 ggataaaacc tgagtttttg aaattctgcc ctgcgttaac gggcggtaag gtgaagaaag
     4801 tgaatcagta tcggtttgaa aacaatctgt aagtgcttga ccaaatccat acgacagaaa
     4861 tgatgcaaga aagattaaaa tggttttagt aatggataac tctgaaataa aagacattcc
     4921 gcatatacct gttactcctg agacaaagag aaggtacgga cgcattgtga tgatataatc
     4981 ttttacaaaa tcaattgaga atgttttatg atatttgctt cgtgtcagcg gttccattta
     5041 tttccttacc caaatagatt attgaaataa cttttgttaa aaatctaatt aagtctcata
     5101 gcaaatttgg caggtttaaa acttagagtt gtcaaaaagt tgtcaaaaaa tggcggctct
     5161 taacaatttg ttaattttta cttaatcttg gcttaacact cagtaaacca gttataagta
     5221 ttttttcacc gaaaatcata ataaaaaagg aaaattatga aatcactctt agctttcgca
     5281 ttaattcaaa taatgattta tatttttagt atgagcaatg caataacaat cggccttatc
     5341 gtcctgggtt ctgcgataat actattcgct ggcgcatatt tatcagcttt agttaaagat
     5401 aaaaagcacc gaagaaaaaa cattagcgca gccttagaat tacagtaaaa attctcagct
     5461 atttttactt agcgttttga acccgtcaaa acgcaggaga attatttgat tttcaatatt
     5521 ccagctctgc ttcttccgcc atttttacta aatttgattg aatgtgttat tataactgct
     5581 taaattaagc ctgtaagaag tattcattaa tcgtgaaagc gttatgaaag aactcttcag
     5641 tgtgcaggaa gtagcaaaac ttttggatat gagtcattcg ggagttttgt atcacattaa
     5701 aaatggatcg ctgaaatcca ctcaggttgg taaaatctat attatttcta aagaagattt
     5761 tgctgatttt cttaaagagc agaagaaaga aaagaagagc aagaaaaaac ctgaagagcc
     5821 attgctattt taattactct ttcgaaaaag ctaataattc ctgtctgtat ttttcaagat
     5881 ctttaatgct tatcccggtt aattctttaa tgcaatagca gtaagcacca ataattggaa
     5941 tgattaaatt ttcttttctg ctataaaatt catcaatcat ttcaaccata aaatttaaat
     6001 caagttcttc agactttcca gactcaccaa gttttgccac gtcatcaata catccaagcg
     6061 gcatttgggg tttattttca tatttaatca tatatctttg attttcaagc atgctcgcaa
     6121 ggtattcaaa tttgtaatcg gtaagtatgt tgttggattg attaagcgtt aaccaggcat
     6181 aaccattttc agcgcctttt gtataacgtt tatcctgtgc agtaagcaga atatttgtgc
     6241 ataatagaaa aaatatgata gaatgatatt tcaaattatc ctttgtttta atgcagacta
     6301 aaattgctaa aaaacacttc aaatctcaac tacaaaaaga ttaaattagc ttataaaatt
     6361 attgcagatt ggatttttaa tgggctcaca tcttctaagt aaatcttcat ttattcgcgg
     6421 tgttcaatgc gaaaaacatc tttatctcta caaatatcac tacaaagaaa tggatcaact
     6481 ttctgatatg cagaaagcaa tttttaaaag agggacagat gtaggtaaac ttgctcagca
     6541 gctttttccg gatggcattg atgcctcacc cgcaacacag tttgaataca ataaagcagt
     6601 tattgctaca aaaaatctga tcgataaaaa gcaaaaagtt atttatgaag cttcctttaa
     6661 tcacagtgat gttttggcag ttgcagatat tattttgaat gaaaaatccg gattaaaagt
     6721 atttgaagta aaaagctcaa catcaatttc agaaacttac attcgtgatg cggctttaca
     6781 atattgggta atttctaatt gcggatataa gattaaagat ttttctatca cctatatcaa
     6841 taatcaatac attagaaaag gtgaacttga gttagataag ctttttataa ccgaatcagt
     6901 tttaaaattg attttgccat tgcagaaatg ggttgaagaa aatgtgaatc gttttaagca
     6961 agtgcttgct aaaaaagcta ttcctaaaat cgatatcgga gagcattgtt atgatcctta
     7021 cacttgcgga ttttatgagt actgcagaaa tcatattcct gaaaattcaa tcttcgatct
     7081 ttcaggagtt catcttaaca aaaaatatga tttttacaga agcggaatta taaagttaaa
     7141 agatattccg gttgaggttg agcttcctaa aaacgcaaag attcaactgg atatttttaa
     7201 aagcaaaatt gatttaatag ataaaaaagc tattaaagaa tttttatgcg atttaaatta
     7261 tccactttat tttatggatt ttgaaacttt tcaagcagcg gtgccgatat ttaataattc
     7321 aaaaccttac atgcagatac catttcaata ttcccttcat tataagaaaa ataaaaaaag
     7381 caagattgat cattataagt ttttagctga aacagggatt gatccgagaa taaaatttat
     7441 tgaaaattta ttacgtgata caaaaagtga gggtgacata ttaacctaca acaaatcgtt
     7501 tgaggttctt agacttaaag aaattgctga agcatttcct aaatacgaaa aagaaattga
     7561 agaacgaata agtcgtatta aagatttaat gctgccgttt cagaaaaaat attactacac
     7621 tcatcagatg catggttcat attccattaa gtatgtgctg cctgctttga ttcccgagct
     7681 tagttatgaa aacctggaaa taaatgaagg tggacttgct tcgattgcat ttgaaagcct
     7741 ttactatgaa actgacttga tgcgaattgc cgatattaga aaaaacttac ttgagtattg
     7801 taaaatggat acatttgcaa tggtaaaact tcttgaaaaa ttggaatcac tttaaaagaa
     7861 aatagtatga aaaaagtttt gtttcctctg ctttttattt ttatcacttt aagcactttt
     7921 gctcaaacag tggacataaa agtaatgact tacaacattc gctgcggatt ttgtgaagat
     7981 tcttctgatg tgaacaactg gagcaagaga aaatatttag tggcttactt aataaaaaat
     8041 cataatcctg atttaattgg tttacaggaa gcagaaatgt ttcaggttaa agagttaatt
     8101 gagatgctgg atgaatatga ttggtacggt gtaagtcgtg aagatggtaa agaaggagga
     8161 gaatcaactg ctattcttta taaaacaaaa agatttaagc ttgaagaaaa atatacaatt
     8221 tggttatctg aaactcccga acttgtttct aaaggctggg atgccgcctt taaaagaact
     8281 gttacaattg caaagctaaa agatttgcaa tctaagaaag agttttatta tttgaacaca
     8341 cactttgatc atataggtga gactgcacga actgaaagct cacgtttgat tgtaaaagaa
     8401 attaataaat actccaaaga ttatccgata attctctccg gagattttaa ttatacatca
     8461 agctcggatg gttataaaat aattaccggt aaactgtttg attcaaaatc agtttctaaa
     8521 aaagaaagtc ttggcggtaa tattacattt aatggatttg gaaaagatat tcagccggat
     8581 aataaaattg attttatttt tgtgaataat aaaattgaag tattaaatca tctgatagac
     8641 actacaactt ttaatggatt gtatccttcg gatcattatc ctgtaattgc agatctttta
     8701 ttaaaataaa ctttttgaaa gttgaagatt attttaaaag tgaaattatc ctatcattat
     8761 taatgactat tcatcaagct atttttgcgc ttatattttt tattataaga cattcttgat
     8821 tctttaattc tgaatcattt attttgcggt cgcaattcta aatccgatta tataaaaaca
     8881 attacaggta acacttgaaa atttcaattt catttttgta cataattata ttcttttcct
     8941 cgcaaatttt tgcgcaatgg aataaagttg aagatataac ttcacctttt gtttattcag
     9001 tactttttga tgctgataac atttttgtcg gaggtgattc actttacatt agcagagata
     9061 ggggattaac ctggcagtca gcaactttaa tcggacaggt aattgagatt accgcactat
     9121 ttaagtatga caacatgatc tttgcaggca catacggaag cggtgttttt atcagcagta
     9181 acaatgggtc atcatggcaa agctataatt cgggattagg tgcatttgct ttatatgcca
     9241 agaaatttgt ttcatctggg gatacaatat tttatgctac cgatggcggt ggagtttact
     9301 atttgctaaa caataccaat gtgtggcagt cttataatca aaatcttcct tcaagtatag
     9361 catggacaat aaatgatatt gcagttacta ataacaacat aattgtaagc gccggcggaa
     9421 gtggttttta ctatcttcgt cccaaaggat cagcagaatg gattgaaaaa agaatgcaaa
     9481 ctccgcaagg aatctttatg accccaaatg cttttctttc aacagggaac attgtttttt
     9541 ccggacaccg ttttggtatt tataaaagcc tggatgaagg aaacactttt gattcagtag
     9601 gtatatccgc aatggatatt gcagttgtta gttttgttat ggatgaaaat agaatttatg
     9661 caggatatac acgatcatct ggaaatgatt tttttgtttg gtactctgac gatcttggtc
     9721 aaagttggaa tatttttgct catgagtttc aatttttgtt aaatctttat atttatgata
     9781 ataaaatctg ggctggaaca aatgatggac tttactatac ccagcttcaa ccatctaatg
     9841 tggagcctat agatattccg cataaattaa aacttgacca gaactatcca aatccattta
     9901 atccaactac aaaaataagt tggcaatctt caattagcgg tcggcataca ttaaaaattt
     9961 atgatgtgct cggtaatttg gttgaaacac ttgtagatga agatagacct gcaggaaatt
    10021 ataatattac atttgatgcg tccagtttat caagcggagt atatttttat aagcttcaaa
    10081 ttggttcata tatcgaaact aagaagatga tactattaat gtagtcatct aattgaacta
    10141 tagagacatt tagtttaagt tatacttaaa ttaaaattaa tttcgggtag ctggaatgaa
    10201 tcttgcggat ggaattcttt ggtacattgt ttttctattc tcaaccgttt ttcacgaagc
    10261 ggcacacgct tttactgccc acaaaatggg tgatagtact gcgcataaaa gcggacaggt
    10321 aagtttaaat ccgattccgc atatcaaaag agaaccgatt ggaacagtta tcgttccggt
    10381 tctttctttt ctcgctgggg gatggatgat tggatgggca agcactccgt ataacgtaca
    10441 atgggcacac gattacccaa agaaagcggc tgctatggct gctgcggggc caatttctaa
    10501 ttttattctc atcattatct cagcagtaat tattcatgtt gggatatcat caggattttt
    10561 ttaccagcca gattcaatca ccatttcagg aatagtttcc ggtgcaaatt ccggctggct
    10621 tgaaattctt gcaaagattg taagcgtaat gttttcactt aaccttatac tttttgtatt
    10681 taatcttctt ccatttcctc ctttagatgg aagcgcaatt ccaccaatgt acttgagtga
    10741 agacgcagga agaaaataca ttgcctttat tcgtaaaccg gtttttgctt tggcaggatt
    10801 atttgttgcc tggaatttat tagatataat tttcaataaa atacacttag ttgtaattaa
    10861 tcttctgtac ccgggaagca attatcatta aatctttcct catttctcct taactttgca
    10921 ctaaaaagga gatatatatg aacatcaaag attcaacatt tcttgtaacc ggcggcagtg
    10981 caggaatagg taaagcaact gctaaactgc ttgttgaaaa aggaggcaaa atcgcaatca
    11041 ctggcagaga taaatcaaag ctggaaaaag ttgcaaaaga aattggtgca tttgcaatcc
    11101 acgcagacgt tgcggtggga agtgatgttc acaaaactta tgaactattt cttaaacagt
    11161 ttggaaaact tgattgtctg atcaataatg ccgggattgg tgacgcctgg aatgaaataa
    11221 ccgaacttga tatgaaagca ttccaaagag tttatgatgt aaatgttttt ggcgctgcac
    11281 ttatggcgag tgaagctgct aaacttttta agaaacaaaa atccggtaac ataattaata
    11341 tcgcatctac agcaggtact aaaggttttg caaacggaac tgtttatgct tcctccaaat
    11401 ttgctttacg tggaatgacg caatgctggc aggctgagtt aagaaaatat aacgttcgcg
    11461 ttatactcgt taatccaagt gaagtaacaa ctgctttcgg tgataatgaa cgtaatgaaa
    11521 agtctgaagt aaaaaataaa ttgagaccat tggaaattgc ccacacaatt gtgaccgcct
    11581 tggaaatgga tgatcgtgga tttattcctg aagttactgt ctgggcaaca aatccatttt
    11641 aatttacaat ttacaatgaa taatgtacaa tgaagaataa ttctgaaact tttaattggc
    11701 tggaggttgc aagggaaatc caacagcttt ctcaaacagg attggctttt gcagttactg
    11761 attacgaaaa aaatcgttat aaacgattaa cagaaattac tgctgagata attgaacatc
    11821 acacgacact tgaaaaggaa tcagtaacaa aaattttaat gaagcatccc ggttatgcaa
    11881 cgccaaagat tgatgttcgt gctgcagtaa tcaaagacaa aaaaatttta ctggtgcagg
    11941 aaactacaga caattgctgg gcaatgcccg gcggctgggc agatgttggt gatatccctt
    12001 cggaggttgc gataagagaa tgtaaagaag aaagtggtta tgatgtaaaa cctattaaag
    12061 ttattggggt ttttgatgca aataggatcg gaggacattt agaatttttt cacgcattca
    12121 aaataatatt tctttgtgaa ttaattggcg gggaagcgag aacaagtgat gaaacaatcg
    12181 atgtgcagtt cttcgatttt gataaacttc ctgcattatc tttaaatcgt acaaacgata
    12241 agcatctaaa tgaaattaag cagcatttaa tagatcccaa acgaaaaact ttttttgatt
    12301 aatatatgcc atcatcaaga gatataatgt atacaacagc gcatgctgaa at
//
LOCUS       DASWBY010000563         4111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_26372, whole genome shotgun sequence.
ACCESSION   DASWBY010000563 DASWBY010000000
VERSION     DASWBY010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26372"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VF870_10195"
     CDS             <1..438
                     /locus_tag="VF870_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9252604.1"
                     /translation="GNILNLDEVLKTLENAVKNHPKTLFIACHLANQNTDLEKLGWLL
                     DLYPNFYADISARYSENAPVPRYTRAFFEKYQDRLLYGTDMGFEKSMYETTFRILESN
                     DEHFYETELFGYHWPLYGFGLNDEVLEKIYSLNSKRILVEKIK"
     gene            complement(945..1017)
                     /locus_tag="VF870_10200"
     tRNA            complement(945..1017)
                     /locus_tag="VF870_10200"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(982..984),aa:Thr,seq:tgt)
     gene            complement(1086..1685)
                     /locus_tag="VF870_10205"
     CDS             complement(1086..1685)
                     /locus_tag="VF870_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252605.1"
                     /translation="MKKIIITVIPIILIFAYLFWPEKVISYPAGQTAPDQPIQKNIVT
                     PKEWQKDEFHIKALAEYKIKARVLSRNNFSIGRESELSPVDFALGWGPMSDQEVIDKI
                     EVTQSNRWYHWETKSYPIPREEISLNSANVHIIPKDESVEEKIDNVYKGSLIEMSGYL
                     VEVTVDDGWHWRSSLRRDDTAGGSCELFWVEDLTILDSE"
     gene            complement(1682..3007)
                     /locus_tag="VF870_10210"
     CDS             complement(1682..3007)
                     /locus_tag="VF870_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9252606.1"
                     /translation="MELLFVFVVILFANFLLAASEIALAAFGETKIDELKEKNDKHVP
                     YFIKLNENHEQVYGSIHLAFTFLTVLISIIGYFLFSGWIFPQITFITNLSPIYSIVIS
                     IIITTILVTLIILVFNVLVPKAIAYRYADSIGKRSVKIILILSSSLKLVTKTITAISN
                     LILLPFKEKINFSDERPSEDEILDIISDGVKSGTIDEADQEIIENIFEFNDLKADQVM
                     IPRTEMIAINLNENDELNISSIIKSGHTLVPVYENSLDNIVGILHTKDLMKSIIEKQS
                     YSLNNLIRPAYFVPETKLISEILKDMQKQGERIAIVTDEYGGTEGVITMEDILEEIVG
                     ELKDKTKSEIQNYSKLPDGKYYVLGSMNIDEFNETFNYQLPESDDYNTVAGFIAEKTG
                     KILNPSEKYEYEGLAFELVKKIKQKMVQFKVTANTGNFEERKIKNRSRE"
     gene            3134..3475
                     /locus_tag="VF870_10215"
     CDS             3134..3475
                     /locus_tag="VF870_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX9252607.1"
                     /translation="MRPKQIKIVDKDKLSLTWSDDTSTTFSLKYLRDECPCAGCKGET
                     ILLKTYRPPKPAILSPEMYKIKSIDVVGDYAIQIVWKDGHNTGIYSWQYLKTLEAGAS
                     NNTQQKYDTLL"
     gene            3472..>4111
                     /locus_tag="VF870_10220"
     CDS             3472..>4111
                     /locus_tag="VF870_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9252608.1"
                     /translation="MISKNELKYFSSLLIKKFRQQEEKFIVEGLKTVEEGLNSSYQCD
                     AVFVTPAFIDSFPDVINILNKKTNRVLEVKSIDFQKISDTKSPQGIAAAFNKNYPKKN
                     LNELKDKLVVLIDNISDPGNLGTIIRTCDWFSVYNILISDQSVEYLNPKVIRATMGSL
                     FHLNIYDEIYENDLANLKSNGYQIVCADTKGKNIFSYKTNKKTLIAFSNESLG"
BASE COUNT         1401 a          686 c          625 g         1399 t
ORIGIN      
        1 ggaaatattt tgaaccttga tgaagtctta aagactcttg agaatgcagt taagaatcat
       61 ccgaaaactt tattcattgc ttgtcatcta gctaatcaga atactgattt agaaaaactt
      121 ggatggctat tggatttata cccaaacttt tacgcggata ttagtgccag atattctgaa
      181 aatgcgcctg tacccagata tacccgtgca ttttttgaaa aatatcagga cagactttta
      241 tatggaactg atatgggatt tgaaaagagt atgtatgaaa caacattcag aatattagaa
      301 tcaaacgatg agcattttta tgaaactgag ctctttggat accactggcc tttatatggc
      361 tttggattaa atgatgaagt attagaaaaa atttacagcc tgaattcaaa aaggatacta
      421 gtagaaaaaa taaagtaaaa gaataaaaac tgacattcga tcaactttca acatttaatt
      481 atttctgctc aacttgcagc cctgcctgcg aatgttctat aatggatgaa tatgctgcaa
      541 cataaataag ttgaaaatat ccctctgtaa aaaactatta aataaaaatt cttaaattat
      601 tattttatta taaataataa agcacagcat tgaaccttca gtcgaaatat tgattctgtt
      661 tattcaagac tacaaccggg atataaaaat cattagaatt atagagctat tttcttatta
      721 aattgatgaa aatgaactac tttgtttttc aatttattta actcgcgcag aagtgtttaa
      781 aatcagttct aattcaaaca aaaaatgtta ataggtaaaa tttactgcca tagtatttgc
      841 catagtaagg gtcaaaaaga gggggtcaaa tggggtcaat ttcagtcagt tgaaattttg
      901 gtcaaaaaaa aagatgcgat ttctttcgat tttcgcatct tttgagctgg ctatgggact
      961 cgaacccgcg acctgctgat tacaaatcag ctgctctacc aactgagcta agccagcatt
     1021 ttcggagagc aaaattaaca tttttaagtc ttctaaacaa gaatcttttt taccttctct
     1081 aaaaattatt cactatctaa aattgttaga tcttcaaccc aaaagagctc acaggatccg
     1141 cctgctgtgt catctcttct gagagaactc ctccaatgcc atccgtcatc tacagtaact
     1201 tcgactaagt atccgctcat ctctattaaa cttcctttat aaacattatc tatcttctct
     1261 tcaactgact catcttttgg tatgatgtgg acattggcac tatttaagga aatctcttcc
     1321 cggggaattg gataggattt tgtttcccaa tgataccatc tgttactttg agtgacttct
     1381 attttatcaa ttacttcctg atcagacatc gggccccagc caagagcaaa atcaactggt
     1441 gataattcgc tttcccgacc aattgaaaaa ttatttctgc taagcaccct tgctttaatt
     1501 ttatattctg caagtgcctt tatatgaaac tcatctttct gccattcttt tggtgtaaca
     1561 atgtttttct gaataggttg atccggagct gtttgtccgg caggatagct gataactttt
     1621 tcaggccaaa ataaataagc aaaaattaaa attataggaa taactgtaat aattattttt
     1681 ttcattctct tgatctattt tttatcttgc gttcttcgaa attccctgta tttgcagtga
     1741 ctttaaattg aaccattttt tgttttattt tttttacaag ttcaaaagca agtccttcat
     1801 actcatactt ttcagatgga tttaaaatct ttcctgtttt ctcagcaata aatcccgcaa
     1861 ctgtattata atcatctgat tccggaagtt gataattaaa tgtttcattg aactcatcaa
     1921 tattcattga gccaagcaca tagtacttgc catcaggaag cttgctgtaa ttttggattt
     1981 cactttttgt tttatctttt aattcaccaa caatttcttc aagaatatct tccatagtga
     2041 taactccttc cgtaccaccg tattcatcag tcactattgc aattcgctcc ccttgttttt
     2101 gcatatcttt aagaatttcg gatataagtt ttgtttcggg tacaaagtat gcaggacgaa
     2161 taagattatt cagggaatag ctttgttttt caataataga tttcattaaa tcctttgtat
     2221 gaagaatgcc gacaatatta tccaaagagt tttcatacac cggcactaat gtgtggcctg
     2281 atttgattat ggaagaaata tttaattcat cattttcatt tagatttatt gcaatcattt
     2341 ctgttcttgg tatcatcacc tgatcagctt taaggtcatt aaactcaaaa atgttttcaa
     2401 ttatttcctg atcagcttca tctattgtac ctgattttac accatcagat attatatcaa
     2461 gtatctcatc ttcagatgga cgttcatctg aaaaatttat tttttcttta aatggcagca
     2521 atataagatt gcttattgct gtaatagttt tggtaaccaa ttttaaactt gaggaaagaa
     2581 ttaatattat tttaactgat ctcttaccta tactgtctgc atacctgtat gcgatagctt
     2641 ttggaacaag aacattaaag actaaaatta ttaaagttac aagaatagtt gtaatgatta
     2701 tagatataac aattgaataa atcggcgata aatttgttat aaatgttatt tgaggaaata
     2761 tccagcctga aaataaaaaa tatccgataa tagaaataag tactgttaaa aaagtaaaag
     2821 ctaaatgaat gctgccgtat acctgttcat gattttcatt aagttttata aaatatggta
     2881 cgtgtttatc attcttttct ttaagctcat caattttagt ttctccaaaa gctgccagag
     2941 caatttcaga agcagcgagt aaaaaatttg caaacaaaat cactacaaaa acgaacaaaa
     3001 gttccattaa attttaaata tccttctaat gttaatagct aacacaaaaa tagtatggtt
     3061 atgttaaatt tacattacat aatttttaat attatgttat tttaagattg taatcattaa
     3121 aaatattaat taaatgcgac caaaacagat taaaatagtt gataaagata agttatctct
     3181 gacctggagt gatgatactt caaccacatt ttccctaaaa tacctgcggg atgagtgtcc
     3241 ttgtgccgga tgcaaaggtg aaacaatatt attaaaaact tatcgtcctc caaaaccagc
     3301 tatcctatcg ccagaaatgt acaagatcaa aagcatagat gttgtgggtg attatgccat
     3361 tcaaattgta tggaaggatg gacataacac aggtatttat tcctggcagt atcttaaaac
     3421 ccttgaagcc ggagcaagta ataacactca gcagaaatat gatacacttt tatgatttct
     3481 aaaaacgaat taaaatactt ctcttctctt ttaattaaaa aattccggca gcaggaggaa
     3541 aaatttattg tagaaggatt aaaaacagtt gaagagggtt tgaacagcag ttatcaatgc
     3601 gatgcagttt ttgttacacc tgcatttata gattcttttc cagacgtgat aaatatcctg
     3661 aataaaaaaa caaacagagt actagaagta aagtcaattg attttcaaaa aatatctgac
     3721 acaaaatctc ctcaaggaat agcggcagca ttcaacaaga attatcctaa aaaaaatctt
     3781 aacgaactaa aagataaact ggttgttctt attgataata tatcggatcc cggtaacctt
     3841 ggtacaataa taaggacgtg tgattggttc agcgtttaca acattctaat ctcggatcaa
     3901 tcggttgagt atcttaatcc aaaagtaatt cgcgctacaa tgggatcgct ttttcatctt
     3961 aacatttacg atgaaattta tgaaaatgat ctggcaaatc ttaaaagcaa tggttaccag
     4021 attgtgtgcg ctgatactaa aggcaaaaat attttttcat ataagactaa taaaaaaact
     4081 cttattgcct tttcaaatga atcattagga c
//
LOCUS       DASWBY010000564         4188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_26384, whole genome shotgun sequence.
ACCESSION   DASWBY010000564 DASWBY010000000
VERSION     DASWBY010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26384"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VF870_10225"
     CDS             <1..799
                     /locus_tag="VF870_10225"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560402.1"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9252609.1"
                     /translation="RRHQKIIEESPSSFVDEITRDKITKAAIQAAKACGYYNAGTIEF
                     LMDSNKEFYFLEMNTRIQVEHPVTEFISGIDLVREQISIAEGNKISFEQDEIKINGYA
                     IECRIYAEDPDNNFLPSTGKILHYKEPNGIGIRVDSGFAEDSEISIHYDPMVAKLICW
                     DRDRNKALRRMNRALSEFQLAGITTNAKFLSSILNSKEFAGGNYDINFIDNLNYDQVS
                     LKAYSENKDEHEIAASVLAAIIKSKRRSAYKQNSSLKSNKWWEQNHE"
     gene            792..1295
                     /locus_tag="VF870_10230"
     CDS             792..1295
                     /locus_tag="VF870_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX9252610.1"
                     /translation="MNDFIVGINGSSKQVKIIDDNFVEVDNVRLNYSLTELNSSKFIL
                     RIENKFYESALWKKNDDVELIQINSTNIDLSIRTTLQEKAYQLLSKSNSSLEKLTIIK
                     SPMPGLVLKILKKAGDNIAKGETVMILEAMKMENEIKSSMDGIISEILVSEGKPVEKY
                     ISLFSLK"
     gene            1380..2612
                     /locus_tag="VF870_10235"
     CDS             1380..2612
                     /locus_tag="VF870_10235"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX9252611.1"
                     /translation="MRLSVFAVLLSTNLFAGGFQLNEHGARAMALGGAFTGIANDPSA
                     VYWNGAGISQLSGTNFLIGTSLIAPSSTFRGVSPAVTEYRGKNLIFFPTHLFVTHKLD
                     EKFSLGFGFTTPFGLGTEWDENWVGKYLAVETELKTFTANPIVTYTPVESFSISAGFL
                     YSFADVLITRKNSLQPILASDAFVHLEGDDMAAFGFTAGLMFKPSKMFSIGASYHSEI
                     KYGFEGTATTTGPDQVASSLPQGAITAELTTPQNIAVGVAVSVSDRVQLSADYQFIGW
                     SSYDKLEVVFTDPEPDVVSSSPRNYEDSYIIRFGASYILNEQVTLMGGAYFDKNPVTS
                     AYLNPSLPDADRIGLSFGIKAQIYEKLAFEGSYLFIRGQEITVTDSEESYTQGGSPFN
                     GTYNTYANIFSLGLVFSL"
     gene            2628..4124
                     /locus_tag="VF870_10240"
     CDS             2628..4124
                     /locus_tag="VF870_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX9252612.1"
                     /translation="MKIKIGLISIILLSALFVISCDDYTELDAPVQNGTSGTANFNRF
                     VTIGNSITSGYQSGALYQSGQIYAYGNLIAKQVGVDYQAPYISDPGFGSRMEVQSLSP
                     FTIKYNTSSGSLLNSTYPAPYNNLGVTGALTYDVLFATNSTNCASALFANTPNPYFDI
                     VLRNNVLNKGSQLQQAISLAPTFITLWIGNNDVLGYATSGGTSPAAPTTTAQFNQLFG
                     GIMQGLKQYTDASGANVVVANIPSVSAIPFFTTVGPVLATNPALAWWQIALAQTASGL
                     PATGLIYASHEGGTNLGQLPYKIGFADSAALLHCTKLITLVGQSYAPLLGRPTGKYYR
                     DYHIPVPLGVDTTKAFGFHPQNPFPDAFVLDSDEITTSNNAVSSFNSTIAALAGANGY
                     GLVDINTKFNQFRAADFTGGTVINGLTFKTTYVSGGLFSLDGVHPSSQAHGIVANEFI
                     KVINTKYGANIPLVDVSTIPGSIYFTGKVSYKDGYPVIPKNAFDHLLF"
BASE COUNT         1386 a          688 c          788 g         1326 t
ORIGIN      
        1 aagaagacat caaaaaataa ttgaagaatc accttcatct tttgttgatg aaattacaag
       61 ggataaaatt acaaaggctg ctattcaggc tgccaaagct tgcggatatt ataatgccgg
      121 aactattgaa tttcttatgg attcaaataa ggaattttat tttcttgaaa tgaatacaag
      181 aattcaagtt gagcatccgg ttactgaatt tatttcgggc attgatttag tgagagaaca
      241 aatctcgatt gctgagggaa ataaaataag ttttgaacag gatgaaatta agattaatgg
      301 ctatgctatt gaatgcagaa tttatgcaga ggatcccgac aataactttc ttccatcaac
      361 cggaaaaata ttacattata aagagccaaa cggaattgga attcgtgtag attccggctt
      421 tgcagaagat tctgaaatat ccattcacta cgacccaatg gttgcaaaac ttatttgctg
      481 ggatagagat agaaataaag ccttgcgtag aatgaaccgg gcattgagtg agtttcagtt
      541 ggctggcatt acaacaaacg caaaattttt aagttctatc ttaaacagta aagaatttgc
      601 aggcggaaat tatgatataa attttataga caatttaaat tacgatcaag tatcactgaa
      661 agcttattca gaaaataaag atgagcacga aattgcagca tctgtgttag ctgctataat
      721 aaaatcaaaa aggagatctg catacaagca aaattcttcc ttaaaaagta ataaatggtg
      781 ggagcagaat catgaatgat tttatagtcg gtataaatgg cagttctaaa caagtcaaga
      841 ttatagatga taattttgtc gaggttgata atgttaggtt aaactactct ttaactgaat
      901 taaatagttc aaaatttatt ctcagaattg aaaacaaatt ttatgaatca gcattatgga
      961 aaaaaaatga tgatgtagaa ttaattcaaa taaatagtac aaatatcgat ttaagtataa
     1021 gaacaacact tcaagaaaaa gcatatcaat tactttctaa atcaaacagc agtttagaaa
     1081 aattaactat aattaagtca cccatgccag gattagtgtt aaaaatatta aagaaagcgg
     1141 gagacaatat tgctaagggt gaaactgtta tgattttaga agctatgaag atggaaaatg
     1201 agataaaatc ttcaatggat ggcataattt cagagatact agtgtctgaa ggaaagcctg
     1261 tcgaaaaata tatttctctg tttagcctta aataatttgt tatttacgaa aaattgctaa
     1321 ttttcgctaa agtacttatc aatcacttta ccaggaggag aaacttgaaa tcttgtttga
     1381 tgcgtttatc agtgttcgca gtattgcttt ccaccaattt atttgctggt ggttttcaat
     1441 tgaatgaaca cggtgccaga gctatggcat taggcggtgc ttttacaggc attgcaaatg
     1501 acccttcagc agtttattgg aatggtgctg gaatctcgca attatcaggg accaattttt
     1561 taattggaac ttcactgatt gcaccaagca gcacttttcg cggcgttagt ccagcagtaa
     1621 ccgagtatag aggtaagaat ttaattttct ttccaactca tctttttgta acacacaaat
     1681 tagatgagaa gtttagtctt ggatttggtt ttacaactcc atttggactt ggtactgaat
     1741 gggacgaaaa ttgggtaggt aaatatcttg cagttgaaac tgaattaaaa acttttacag
     1801 ctaatccgat cgttacatac actcctgtag aaagtttttc aatcagtgct ggatttcttt
     1861 acagttttgc agatgttctt atcacacgaa aaaattcatt acagccaata ttagcttcag
     1921 atgcttttgt tcatcttgaa ggtgatgata tggcggcatt tggctttacg gcaggcttaa
     1981 tgtttaaacc ctcaaaaatg ttttcaatcg gtgcttcata ccatagtgaa attaaatatg
     2041 gatttgaagg cacagcaaca accaccgggc ctgatcaagt tgcttcaagt cttcctcaag
     2101 gtgctattac ggcagaattg acaacgccac aaaatatagc agttggtgtg gcagtaagtg
     2161 taagtgatag agtacaactt tcggcagact atcagtttat aggatggtca agttatgata
     2221 aattagaagt agtctttaca gatcctgagc ctgatgttgt tagctcaagc ccaagaaatt
     2281 atgaagattc ctacatcata agatttggtg caagttatat cttgaatgag caggttacac
     2341 taatgggcgg tgcttatttt gataaaaatc ctgttacttc agcatatcta aatccatcat
     2401 taccagatgc tgatagaata ggtttaagtt ttggaattaa agctcagata tatgaaaaac
     2461 ttgccttcga aggatcgtac ttattcataa gaggtcaaga gataacagtt actgattctg
     2521 aggaatctta tacacaaggc ggaagtccat ttaatggtac atacaatact tacgctaata
     2581 ttttttcact cggtttagtt tttagcttgt aaggaggaag atataatatg aaaataaaaa
     2641 taggtctaat aagtataatt ttattgagtg cactttttgt tataagttgt gatgactata
     2701 cagaattaga tgcacctgta caaaatggaa ctagcggcac tgcaaatttt aatagattcg
     2761 taactatcgg aaacagcata acttctggat accagagcgg agctctttac cagagtggtc
     2821 aaatttatgc gtatggtaac ttaatagcaa aacaagttgg tgtagattac caagctcctt
     2881 atatatccga tccaggtttt ggtagtagaa tggaagttca gagcttatct ccatttacta
     2941 ttaaatataa tacttcaagc ggctctttat taaattcaac ttatccggcg ccatacaata
     3001 atcttggcgt cacaggtgct ttaacatatg atgttttgtt tgccacaaat tcaactaact
     3061 gtgcatctgc attatttgct aatacgccta atccatattt tgacattgtt ttaagaaata
     3121 atgtcttaaa caaaggatca caacttcagc aagcaatttc cttagcccca acatttatta
     3181 cactttggat aggtaataat gatgtgctag gatacgctac tagcggtgga acttctcctg
     3241 ctgctcctac gactactgct cagtttaacc aattatttgg tggtattatg cagggtttaa
     3301 aacaatatac agatgctagt ggtgccaacg tagttgttgc taatatccca agtgtcagcg
     3361 ccattccatt tttcacaacc gttggacctg ttttggcaac aaatccagct ttagcctggt
     3421 ggcaaattgc tctagcccag actgccagtg gtttacctgc caccggactt atttatgcaa
     3481 gtcacgaagg aggtacgaat ctgggacaac taccatataa aatcggattt gctgattctg
     3541 ctgccttgct acattgtact aaacttataa ctttagtagg ccagtcttat gcaccattgt
     3601 taggtcgacc gacaggaaaa tactatagag attaccacat acctgtacca ctcggtgtag
     3661 atacaactaa agcttttgga ttccatccac agaatccttt tccggatgca tttgttttag
     3721 attcagatga aattacgact tcaaataatg cagtatcttc tttcaattca accattgcag
     3781 cattagcggg tgctaacgga tatggattgg ttgatataaa tacaaaattc aatcagttta
     3841 gagcagcaga ttttacagga ggaacagtta taaacggtct aacttttaaa accacttacg
     3901 tttctggtgg tttatttagt ttagatggtg ttcatccatc cagtcaggct catggaatag
     3961 tcgctaatga atttattaag gtcatcaata caaagtatgg agctaatatt ccattggtag
     4021 atgtatcaac tattcccgga agtatttact ttacaggaaa ggtaagctat aaagatggct
     4081 atccggtaat tcccaaaaac gcatttgatc atttactatt ttaattaata ctagtaatcg
     4141 cttaaaggga tggtttacca tccctttttt ttatactttt tgaaaaac
//
LOCUS       DASWBY010000565         6893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_477149, whole genome shotgun sequence.
ACCESSION   DASWBY010000565 DASWBY010000000
VERSION     DASWBY010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477149"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VF870_10245"
     CDS             <1..445
                     /locus_tag="VF870_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9252613.1"
                     /translation="EAKGNQIYSVSPDTTVYDALKLMADKNIGAVVVLEGKKLFGIMS
                     ERDYARKVVLKSKFSKEVLVKEIMSSEIFCIDIDQTIPNTQAIMIQKRIRHLPVIDKD
                     ELVGIVSIGDIVKASIDNKTFLIDQLITYIRGIPTPQKEQIFEII"
     gene            545..1615
                     /locus_tag="VF870_10250"
     CDS             545..1615
                     /locus_tag="VF870_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX9252614.1"
                     /translation="MESHRGEKHIIVLHEYPDPDAIASGYAHKIISTSFDIETDIIYS
                     GTVSHPQNKALIRSLDCNLIPYEKGFDFSVYDSAVFVDHQGTTVPGIVNGLTEAGVQL
                     LIMIDHHEKQNKYNFEYVDICKTGSTATIYTRFLSQNLIKLDKSDKSQVIMATALMHG
                     IITDTNSFIRANELDLQAAAFLSNYWDTELLKLIISQSRSKQIMDIIQMGLKNRTNVE
                     NHSIAGVGYLRAEDRDAIPEAADFMLTEENVHTAIVYGIVRNDKNEEILIGSLRTNKF
                     TLDPDEFIKTAFGKDTNGQFFGGGKQSAGGFSIPIGFLSGDNQEKFNQLKWDVFDKQI
                     KTKILLKIGVNSEQSVPDDKSQ"
     gene            1945..2733
                     /locus_tag="VF870_10255"
     CDS             1945..2733
                     /locus_tag="VF870_10255"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEX9252615.1"
                     /translation="MKKLIMLSMLIAALFIGCSDDSTGPASGKGSLKMYLVDSPSSFD
                     SVIVFVSRVEVHKSGSDDNSGWFVINNTLRSFDLLQLRNGASAVLGDSILASGDYTQI
                     RLILTNGNYVIDNGVKHDLIIPSGSQTGIKLNHGFTIEPNSLYELILDFNVDRSIHIT
                     GNGQYMLRPVIRCMSMITSGTISGQVLPLDAQAIVFTTIGSDTVTTYPDALGFFRLMA
                     LPQGTYNLEINPVNLAYKDTVITGVNVLANQNNNIGLVELRSNP"
     gene            complement(2887..3291)
                     /locus_tag="VF870_10260"
     CDS             complement(2887..3291)
                     /locus_tag="VF870_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9252616.1"
                     /translation="MKSIYSEGIKSDETTIISSGVRIEGKVASTGNIRIDGEIQGDIS
                     SKSNVIVGESGTVNGQIDADSITIAGKVSGTLKAKDKITLEAKANFKGDLFTKILVVE
                     AGAKFDGESKMSGVNETSEMIMNPKPNISSAK"
     gene            complement(3350..3799)
                     /locus_tag="VF870_10265"
     CDS             complement(3350..3799)
                     /locus_tag="VF870_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252617.1"
                     /translation="MKKIILISAVFILLTNFSYSQDHGFGLGIVLGEPTGLSAKLYTG
                     STNAFDFASAWSFKGDGNLLVQADYVWHNSVTRASSGKLMFYYGLGGRVIFQDDPLVG
                     ARIPVGLDYQFTTAPIDIFIEIVPILDLIPSTDFDLGGGLGVRFWFN"
     gene            4884..5735
                     /locus_tag="VF870_10270"
     CDS             4884..5735
                     /locus_tag="VF870_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9252618.1"
                     /translation="MAKQYLDAWNAHDADAIIKLFTSAGTYSDPSAGEITGDAIGANA
                     RGLWSAFSDLTFEVVSLVETTPGRVVAEWVMKGTNTGSFHGLPATERTISLAGVDVME
                     IGNDGIKTVKGYFDTKTLSEQLGLQVLVQPYKLGPFSFGFSVAVQSGRKTKPGAFGIT
                     TIWNSDSQTEEIRDLTRATATEMLGMEGFIGLTTMRIGGRGITISAWEKPEHTMQLKK
                     EGTHSEAMRKFWTELSDSAFTSVWVPHHINPMWVRCTVCREMNSYNKNSGVCSCGEPL
                     PEAPPYF"
     gene            5761..6240
                     /locus_tag="VF870_10275"
     CDS             5761..6240
                     /locus_tag="VF870_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9252619.1"
                     /translation="MAKSKLLEMNNVGIVVESLDNTITFFTEIGLKLEGRMMVEGEWA
                     GRVTGLGNQSVEIAMMVTPDGHSRLELSRFLIPNIISDHRTAPVNSLGYLRIMFRVDN
                     LDELLLRLTRYGAEIVGEVVEYEGIYRLCYIRGIEGLLIGLAEQLSNKTTTDILDNP"
     gene            6346..>6893
                     /locus_tag="VF870_10280"
     CDS             6346..>6893
                     /locus_tag="VF870_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX9252620.1"
                     /translation="MRKIISFMHISLDGFVAGPNGEMNWIKVDEEIFDFVGKRISEGD
                     TALYGRVTYQMMENYWPTAADKPSASRHDIEHSKWYKKVHKVVLSKTLKDSELTKTQI
                     ISDNISDRINELKHKSGNDILLFGSPTATHSLIQLNLIDGYWLFVNPIILGRGILLFV
                     DIKDKINLKLLSTRPFTCGVTEL"
BASE COUNT         2246 a         1175 c         1307 g         2165 t
ORIGIN      
        1 agaagcaaaa ggtaaccaaa tctattcggt ttcaccggat acaactgttt atgatgctct
       61 taaactaatg gctgacaaaa atatcggcgc agtagttgtg cttgaaggga aaaaattatt
      121 cgggattatg agtgaaagag attatgcgag aaaagtggtt ctcaagtcta aattttcaaa
      181 agaagttttg gtaaaagaaa ttatgtcatc tgaaatattt tgtattgata ttgaccagac
      241 gatccctaac actcaagcaa ttatgataca gaagcggatc agacacctcc ctgttattga
      301 taaagatgaa ctggtcggta tagtatccat cggtgatatt gtaaaagctt caatagataa
      361 taaaacattt ttaatagatc agctgattac gtatataaga ggtattccca ccccgcaaaa
      421 ggagcaaata tttgagataa tttagtgatt ataaacatag aattactgtt tatattttta
      481 gtttaaatga tattaacatt caaataggtg tgtgatgagt actcaaaagg aacttcggtc
      541 agtgatggaa tctcatagag gcgaaaagca cattattgtt ttacacgagt atccggatcc
      601 tgatgcgatt gcatccggat atgctcataa gattattagc acttcttttg atattgaaac
      661 tgatatcata tatagcggaa ctgttagcca tccacaaaac aaagccttaa tacggtctct
      721 tgactgcaac ttaatccctt atgaaaaagg ttttgacttt agtgtatatg attcagcagt
      781 gtttgttgat caccaaggaa ctacggttcc cggaattgta aacggattaa ctgaagcagg
      841 cgttcaattg ctgattatga ttgatcatca tgaaaaacag aataaatata actttgagta
      901 tgtagatatt tgcaaaacag gctcaactgc aacaatctat accaggtttc taagccagaa
      961 tttgatcaag ttggataaga gtgacaaaag tcaggttatt atggctactg ctctaatgca
     1021 cggaataatt acggatacaa attcttttat aagagcaaac gaattagatt tacaagccgc
     1081 agctttttta agtaattact gggatactga attgctcaaa ttaataatca gccagtctcg
     1141 ctcaaaacaa ataatggata ttatacaaat gggattgaaa aatcgtacta atgtagaaaa
     1201 tcattctata gctggtgttg gttatttacg tgccgaagat cgtgatgcta ttcctgaggc
     1261 ggcagacttt atgttgacag aagagaacgt acacactgca attgtgtatg gtattgttag
     1321 aaatgataaa aatgaagaaa ttttgatagg atcattacgt actaataaat ttactctgga
     1381 tccggatgaa ttcataaaaa ctgcttttgg aaaagatact aacggtcaat ttttcggcgg
     1441 tggtaagcaa tctgctggcg gcttttctat cccaattggg tttctttcgg gcgacaacca
     1501 ggaaaaattt aatcagttga aatgggatgt gtttgataaa cagataaaga caaaaatttt
     1561 gctaaagatt ggtgtaaatt ctgaacaatc tgttccggac gataaatctc aataattctg
     1621 ttcatattta aaatcgaaca aataataaac cctgaaaccg aagcttcagg gccttaaata
     1681 aaacggttct tctttttatt ttacattttc acttcttgca tctttcctct catttcaaca
     1741 aaatcttatg ctgataatgt gattgcaggt acaattcatc tgacaataaa aatagatgtt
     1801 attgataaat attattatat ataaatattt atttgcaaaa ataattcgcg tcaatttatt
     1861 gtatcatatt tttcacttgc aatttattca aactctttat acactctttc tggaatttca
     1921 ttcattcact ttaggagaat taatatgaaa aagctaatta tgttatctat gctaatcgca
     1981 gctctattta ttggttgttc ggatgactcc accggacctg catcagggaa aggttcactt
     2041 aaaatgtatt tggttgattc accttcatca tttgattctg taatagtttt tgttagcagg
     2101 gttgaagtcc ataaatcagg cagcgatgat aactcaggat ggttcgtaat caacaacact
     2161 ttaagatcat tcgatttact tcagctaaga aatggggcta gtgcagtgtt aggtgattct
     2221 attttagctt ccggagatta tactcagatt agactgattc taactaacgg aaactacgta
     2281 atagataatg gtgttaagca cgatttaatt atcccaagcg gttcacaaac aggtattaaa
     2341 ttaaatcacg gatttactat agaaccaaac tcactctatg aactgatttt ggattttaat
     2401 gtcgatagat caattcatat aactggcaat gggcagtata tgctccgacc agtaattcgt
     2461 tgtatgtcaa tgattacttc tggaacaatt tcaggtcagg tgcttccgtt ggatgcccag
     2521 gcaattgttt ttacaactat aggatcggat acagttacca cttatcctga tgctttggga
     2581 tttttcaggt tgatggcatt accccaaggt acttataatc tggaaattaa cccggtaaat
     2641 ttagcataca aagacacagt cataacaggt gtaaacgtac tggctaatca aaataacaat
     2701 ataggactcg tggagcttcg atctaatcca taataaaatt tgttcgacta tttagccctt
     2761 caactgaagg gctttttagt ttgattcctg gttcatacta atgaacagaa tgcttatcgc
     2821 agaaaaaagc atttatcaat cggataagtt aaaataataa cttaaggatt tttattcttt
     2881 gtggtactat ttagcagagc tgatatttgg tttgggattc ataatcatct cagaagtttc
     2941 attaacaccg ctcattttac tctcaccatc aaatttagct ccagcttcaa caactagtat
     3001 ttttgtgaat aaatctcctt tgaaatttgc tttggcttct agagtgattt tatcctttgc
     3061 ttttagagtc ccagatactt ttcctgcaat tgtaattgaa tctgcatcaa tctgaccgtt
     3121 tactgttccg ctttctccga ctattacatt actcttcgat gaaatatctc cttgaatctc
     3181 accgtcgatt ctaatattac cagtactcgc aacttttccc tcaatgcgta ctccgctgct
     3241 tattatggtt gtttcatcag atttaatccc ctctgaataa atacttttca acacatcttc
     3301 tccttattaa aatagatgat aattaaaaat tatgatattt atttgtccct tagttgaacc
     3361 agaatctgac gccaagtcca ccacccaaat caaaatctgt ggatggaata agatctagta
     3421 ttggtactat ttcaataaaa atatcgattg gtgctgtggt aaactgataa tctaatccta
     3481 ccggtattcg ggcacctaca agcggatcat cctgaaatat taccctgccg cccagtccat
     3541 agtaaaacat tagtttaccg gatgatgccc ttgtaacaga gttatgccat acataatcag
     3601 cctgaacgag caaattgcca tctcctttga atgaccaggc agatgcaaag tcgaatgcat
     3661 tggtactgcc tgtatataac ttagcactta atccggtcgg ttcaccaaga actataccca
     3721 aaccaaatcc gtggtcttga gaataactaa aatttgtgag caaaataaaa actgctgaaa
     3781 tcaaaattat ttttttcatt tccagtcctt ttgtagttat tgaattttgt ctatcgttta
     3841 atcatataat aaaaatttga tcttgaaatt acaacctgta tttaactgca taattgttac
     3901 ataaaatagt tgacagttaa tttctataag tattcagcca ttttgaagat agttaagtaa
     3961 tttagagata attttttacc ctttatcaat atcaccttat aatacgataa ggctatttct
     4021 ttctggaaat gagttctttg tgttaattcg cagcttaaaa accagatcga ctttgccaaa
     4081 gcattggatt tgggattttt gatttctgaa tacaaaccag gcccaaaatt cctaattaaa
     4141 aaggaacaac ttatatttat tctaacaaac catgtattct ttgtgctgaa atcatatcct
     4201 gatgtttggt atttacacag ggagcattac ttaaagattt aatcttacat attatattat
     4261 ataataaaat tcactactat ctggagattc cgaaatcact ataagttgat agacattata
     4321 taaaaaaaac ctgaaacaga agtttcaggg ttctgtctta gtatcagggc tggggggaac
     4381 cctttcctaa gtgcgtatca aacataagta aaagcaggat aaaaaataaa cctgttaggt
     4441 caacaaaagt tttccttttt gatcatccct ctctaagcac agccgaagga tttaattatt
     4501 ttcggcaatc agtgccttaa tgctggtaga caggtaaaaa agagaatacc gtttgagcca
     4561 gatgtcatcc agacgcagtc gaaggatcga gagagagttc attcgcctta gtgtgatgaa
     4621 aggaaattct acatatgatt tgtttcacag aattaattat ttgatagctt ttttagatta
     4681 cattttgtat taagtatctg aaatccaaat attatttaat acgcaatagg taaagaatta
     4741 ggttatgcaa aaaagtttat tcaacataga ccagatgacc tcaacgaact caacaacatt
     4801 ccaggctgac ttttgttgtt gaaaccatta atacaatgaa tggaaaaagt taacgaaaga
     4861 aaccaaaaat gacttacatt gatatggcaa agcaatatct ggatgcttgg aatgcccacg
     4921 acgctgacgc gatcattaaa ctcttcacct cagccggcac gtatagcgat ccttctgctg
     4981 gtgaaattac aggcgatgcg atcggcgcaa acgcgcgagg cttgtggagt gcattttcgg
     5041 atttaacatt tgaggttgtg agtcttgtag aaaccacgcc gggcagagtt gtagctgagt
     5101 gggttatgaa aggtaccaac acaggatcat ttcatggttt gccggcaact gaacgtacca
     5161 tttcgcttgc tggagtggat gtgatggaga tcggaaatga tggtattaag actgtgaaag
     5221 gttactttga tacaaagact ttgtcggaac agttaggatt acaggtattg gttcagcctt
     5281 acaagctggg tccattttcg tttggctttt cggttgcagt tcaatcgggc aggaagacta
     5341 agcccggtgc atttgggatc acgacgattt ggaactccga ttctcagaca gaagagatcc
     5401 gcgatttaac cagggcaact gctacagaga tgttgggcat ggaagggttt attggattga
     5461 ccacaatgag gattggcgga cgcggaataa cgatttcagc gtgggaaaaa ccggagcata
     5521 ctatgcagtt aaaaaaagag ggtacgcatt cagaagcaat gcgaaaattc tggacggagc
     5581 tgagcgattc agcctttacg agtgtttggg tcccgcatca catcaatcca atgtgggtgc
     5641 ggtgcactgt gtgccgagaa atgaattcat ataataagaa ttcgggtgtg tgtagttgcg
     5701 gcgaaccatt accggaagct cccccgtatt tttgaagaaa caacataaaa acataaaact
     5761 atggcaaaaa gtaaattatt ggaaatgaac aatgttggaa tcgttgttga atcactcgat
     5821 aacacaatta cttttttcac tgagatagga cttaaacttg aaggtcgaat gatggttgaa
     5881 ggtgaatggg ctgggcgtgt tactggtctt ggtaatcagt ctgttgagat tgcaatgatg
     5941 gttactcccg atggacacag ccggcttgaa ctttcacgat ttctcattcc aaatattata
     6001 tcggaccata ggacggctcc cgtaaattct cttggatatc ttcgcattat gttcagggtt
     6061 gataatcttg atgaattgtt attgagactt accaggtatg gtgctgaaat cgttggagaa
     6121 gttgttgaat atgagggcat ttatagactc tgctacattc gtggaataga aggactccta
     6181 atcggattag cggaacaact cagtaataaa acaacaacgg atattttaga caacccttaa
     6241 aagaaaattc ttgttgtgac ggcggcttga gaattaaaaa tatgagatta attcaagtaa
     6301 taaaaacagc acataaaatc ttgtaaacac taaacaaata acattatgag aaaaataatt
     6361 tcatttatgc acatttcact tgatggtttt gtagcaggac caaatggaga aatgaactgg
     6421 attaaagttg atgaggaaat ttttgatttt gtaggtaagc gaataagcga aggcgacact
     6481 gccctatatg gccgagtaac ttatcagatg atggaaaatt actggccgac tgctgcagat
     6541 aaaccgtcag catccagaca cgatattgaa cattcaaagt ggtataaaaa agttcataaa
     6601 gtcgttttat caaaaacatt aaaagattcg gaattgacta agactcaaat tatcagcgat
     6661 aatatttcgg acagaataaa tgaactaaaa cataaatcag gtaatgacat tttgcttttt
     6721 ggtagtccga ctgcaactca ttcacttatt caactgaatt taattgacgg ctattggcta
     6781 tttgttaatc caattattct tggacgaggc attctcttgt ttgtagacat caaagacaaa
     6841 ataaatttaa aacttttaag tactcgtcca tttacttgcg gggtaactga act
//
LOCUS       DASWBY010000566         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_26486, whole genome shotgun sequence.
ACCESSION   DASWBY010000566 DASWBY010000000
VERSION     DASWBY010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26486"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VF870_10285"
     CDS             <1..482
                     /locus_tag="VF870_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9252621.1"
                     /translation="SEEDWDQAFKQVLLSVVRFSNLVIPKMIEQEWGRIINITSIAVK
                     QPVHNLMLSNSLRAGVTGFAKSLSNEFGGKNITINNVAPGYTLTHRLYELAVNRAKSS
                     GKSHEEILVEMAKDVPMNRLGGPEEIAAMVVFLASQKAGYITGTTISVDGGSTRAIF"
     gene            550..2100
                     /locus_tag="VF870_10290"
     CDS             550..2100
                     /locus_tag="VF870_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9252622.1"
                     /translation="MKQQIELAIPPEEINLPEILSIKSSQNLKLDKEKITAVIPIKKS
                     VDARSRHIVFRILVDVYIDENPEDKKLITYNPVTDSKKVIIVGFGPGGMFAALKLIEL
                     GIKPIIVERGKDVQERRRDIRAVQQDHIVNPNSNYCFGEGGAGAYSDGKLYTRSTKRG
                     DVKKIIDVFIQHGADPEIAVDTHPHIGSNRLPKIVAAIRKTILDSGGEIHFNSKVDDL
                     ILDDNKIIGVKINSEQEIFGDAVILATGHSARDIYYLLNRKKIRIDPKPFAMGVRIEH
                     PQALINEILYHTKEKPPNLPSAGYTLVTEIEERGVYSFCMCPGGIIVPAATAPGEIVV
                     NGMSLSKRNSPFANSGFVVEVTKQEWTKYEKYQPFAALLLQEELEQKAFELANNTQSA
                     PAQRVTDFVAEKISSSLPKCSYIPGLTSAPLHKELPPFIALRLRKALFNFDRKMHGYY
                     TEDAVLVGVETRTSSPIRIPRDRETLMHTEISGLFPCAEGSGYAGGIVSAAIDGENCA
                     MAAERFLK"
     gene            complement(2136..>2565)
                     /locus_tag="VF870_10295"
     CDS             complement(2136..>2565)
                     /locus_tag="VF870_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252623.1"
                     /translation="FLFSILFFSTFISAQNIAVHDMIGKKQNEVIKVYGTPVHKDNSN
                     PEMLCMYYKTNNSTMIFVANAAGVYQAEATKTLDTEPAARNEIDVFISNSIEHGFSVD
                     TVTTSDFHLSKKGTKVDLQVSDNKLSNKYDIRVKAVKSED"
BASE COUNT          871 a          422 c          489 g          783 t
ORIGIN      
        1 tttccgaaga agattgggat caggctttta aacaagtttt actgagcgta gtaagatttt
       61 ctaatcttgt tattccgaaa atgatagagc aggagtgggg tagaataatt aacataactt
      121 ctattgctgt taaacagccg gtgcataatt taatgctatc aaattcatta cgtgctggcg
      181 taacaggatt tgcaaagtct ttgagtaacg agtttggagg taaaaacatt acgataaaca
      241 atgttgcccc tggttataca ttaactcaca gattgtacga gctcgctgtt aatcgtgcta
      301 aatcatccgg caaatctcac gaagaaatat tagttgaaat ggcaaaagat gttccaatga
      361 atcggcttgg aggtccggaa gaaatcgccg cgatggtagt ttttctggca tcacaaaagg
      421 ctggttatat cacaggtaca accatctctg ttgatggtgg ttctacaaga gctatctttt
      481 aataattaag atttcaaatt atagagtaaa ttcataatta tgtaaatttc ctcataagta
      541 gaggacaata tgaaacaaca aattgaattg gcaatcccac ctgaagaaat taatcttcct
      601 gaaattcttt ctataaaatc atctcagaat cttaaactag ataaagaaaa gatcacagca
      661 gtaattccca taaaaaaatc agtcgatgca agaagcaggc atattgtatt tagaattctg
      721 gttgatgttt atattgatga aaatcctgag gacaaaaaac taattacgta taatccggtt
      781 acagattcta aaaaagtgat aattgtagga ttcggaccag gcgggatgtt tgctgcgtta
      841 aaattaattg agcttggtat caaaccaatt attgtggaac ggggtaaaga tgtgcaggaa
      901 cgaagacgcg atatcagagc tgtccaacag gatcacattg taaatccaaa ttctaattat
      961 tgttttggtg aaggtggcgc aggcgcttat agtgatggta aactctatac tcgatctaca
     1021 aaaagaggtg atgtaaaaaa aattattgat gtttttattc agcatggcgc agatcctgaa
     1081 attgcagttg atacgcatcc gcatattgga tcaaacagac ttccgaaaat tgtagctgca
     1141 ataagaaaaa ctattctgga tagtggcgga gaaattcatt tcaactccaa agtggatgat
     1201 ttaatattag atgataataa gataattgga gtaaaaataa attctgagca ggaaattttt
     1261 ggtgatgcag taattttagc aaccggacat tcagccagag atatttatta tcttttgaac
     1321 agaaaaaaaa ttagaattga tccaaaacct tttgcgatgg gtgtcagaat agaacatccg
     1381 caggccttaa ttaatgaaat actttatcat acaaaagaaa aaccacctaa tctgccttca
     1441 gcaggttata cacttgttac agagattgag gaaagaggag tttattcatt ttgcatgtgt
     1501 cccggaggaa taatcgttcc ggcggctact gcgcctggtg aaatagtggt aaatggaatg
     1561 tcattatcaa aaagaaattc accgtttgca aactctggtt ttgttgttga agtaactaag
     1621 caggaatgga caaagtatga aaagtatcag ccatttgccg ccttgttact gcaggaggaa
     1681 ctggaacaaa aagcatttga gttggctaac aatacacaaa gtgctcctgc tcaacgtgtt
     1741 acagattttg tagcagaaaa aatatcatct tcattaccta aatgttccta cattccagga
     1801 ttaacatctg caccactgca taaagaacta cctccattca tagctttaag attaaggaaa
     1861 gcactcttta attttgatag aaaaatgcac ggatactata ctgaggacgc tgtattagtt
     1921 ggagttgaga caagaaccag ctcaccgatt agaattccac gtgacaggga aacattaatg
     1981 catacagaaa tctcaggttt atttccttgt gctgaaggct ccggctatgc tggtggaatt
     2041 gtttctgctg ctattgatgg tgaaaattgt gcaatggctg cagaaagatt tttgaaataa
     2101 actaaaaatt aattcttatt caattctatt gaaagctaat cttcgctttt aacagccttc
     2161 actcttatat catatttatt tgaaagctta ttatcgctaa cctgcaaatc aacctttgta
     2221 cctttttttg ataagtgaaa atcagaggtt gtaacagtat ccacactaaa tccgtgttca
     2281 atagaatttg aaataaacac atctatttca tttctggcag caggttcagt atctaaagtt
     2341 tttgtagcct ctgcctggta aactcctgct gcatttgcaa caaatatcat tgtactatta
     2401 ttagttttat agtacatgca aagcatttca ggattactat tatccttatg aacaggagtt
     2461 ccatatactt taatcacttc attttgcttt ttcccaatca tatcgtgtac cgcgatattt
     2521 tgtgcactga taaaggttga aaaaaataaa atagaaaata aaaat
//
LOCUS       DASWBY010000567         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_208181, whole genome shotgun sequence.
ACCESSION   DASWBY010000567 DASWBY010000000
VERSION     DASWBY010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208181"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1290)
                     /locus_tag="VF870_10300"
     CDS             complement(295..1290)
                     /locus_tag="VF870_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9252624.1"
                     /translation="MKNKEFVGIDVSKNVLDVFILSCKFHFTVPNSPEGFAQLLEIVS
                     SKLKDSFARVFFCFENTGRYSRLLSVFLQDGGFDFAALDALDLKRSMGLTRGKSDKKD
                     ARTITIYAWRRRDELTTTKLAGPVLDQIRQLLTLRDKLIRHRTAFKNTSRDLHDGYFE
                     GEFDFIKERQESMLSHLQQEIDLVDKEIIRIINLQNDILINYNLLRSIPGIGKVLAVY
                     LIALTHNFTRFDTPRKFSCYAGIAPFEYSSGSSVKGKTKVHPCANKHIKSLLNMAAMA
                     SIQINGEYKMYYQRRLAEGRNKMSTLNIVRNKLVFRAFAIVKRGSPYIDLHKFAA"
BASE COUNT          580 a          330 c          320 g          488 t
ORIGIN      
        1 gactttatct aattaaactt gaaaatggta aaacttttga atttaaaaag ataataaaag
       61 aatagtcgac aaacacaagc tggcacattg tcatcgttca aagtcaacgc taaaaatcaa
      121 aacccagaaa tgtgtctgct tgttatcaac gctttttgca gacattaaaa agctattttt
      181 ttgtctgaag aactgaatga tcgaattgaa aatttatgtc agaccttcaa cagaatagtt
      241 cgcccagttg gtaacactgt tctaaggcaa aacaaaattt ttgatcaaaa aagatcaagc
      301 agcaaatttg tgaagatcga tatacggaga tcctctttta acaattgcaa atgcccgaaa
      361 aacaagctta ttacgaacaa tgtttagtgt actcatctta tttcgaccct cagccagtcg
      421 ccgttgataa tacattttgt attcaccatt tatttgaatg gatgccatag ctgccatatt
      481 tagtaaagat ttgatgtgtt tatttgcaca tggatggacc ttcgttttgc cctttacgga
      541 agaccctgaa ctatattcaa atggtgcaat gccggcataa cacgaaaatt tgcgtggggt
      601 atcaaagcgt gtgaaattgt gagtaagtgc aataagataa acagccagca ctttaccgat
      661 cccaggaatc gaacgtaaca gattataatt gatcaagata tcattctgaa ggttgatgat
      721 gcggattatt tctttatcca caaggtcaat ttcctgttga agatgtgaaa gcattgattc
      781 ctgtcgttct ttaatgaaat caaattctcc ttcaaaataa ccatcatgca ggtcccggga
      841 ggtattttta aaagcggttc tatgcctgat aagcttgtcc ctaagagtaa gcagctgcct
      901 tatctgatcc agaacaggcc cggccaactt tgtggtggta agctcatccc gtctgcgcca
      961 ggcataaata gtaatggtac gggcgtcttt tttatccgat ttgccgcgag taagccccat
     1021 ggaacgttta aggtccaagg catcaagagc agcaaaatca aaaccgccat cctgcaggaa
     1081 tacgctcaac agtcggctgt atcttccagt gttttcgaaa cagaaaaaga ctcgggcaaa
     1141 ggaatcttta agttttgagg aaacaatctc caggagttgg gcaaaacctt ccggagaatt
     1201 gggaacagtg aaatggaatt tacaacttaa aataaataca tcaagcacat tttttgacac
     1261 atcaatgcca acgaattctt tgtttttcat acctttacat ttacgttaca caatagagaa
     1321 gttgctgaaa ctaacaactt taaaagaccc tgggtctaaa attctaaatg gttcttagca
     1381 acttagagag gaatggggac tgacagtgtt aataggtctg gaaacctttc gtgtatacaa
     1441 gttcacccca ttccttttac ttaatgaaca agttaacaac aataaacaaa gaaaagaaaa
     1501 agtagcaaaa agaaaagaaa atcagctaca tacgcattct ttgttcataa attttgttaa
     1561 taacccaata caaatataaa ggcgtgtata agccattgct ggttacaggt ttgctaagtt
     1621 caacacactt ggtcacctag tggtaacttg acaacaaatc gcacagatca cgcagcggct
     1681 cataaacgcg gacgttatgt tcaaccttaa aaattact
//
LOCUS       DASWBY010000568         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_738118, whole genome shotgun sequence.
ACCESSION   DASWBY010000568 DASWBY010000000
VERSION     DASWBY010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738118"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..474)
                     /gene="rpmA"
                     /locus_tag="VF870_10305"
     CDS             complement(217..474)
                     /gene="rpmA"
                     /locus_tag="VF870_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX9252625.1"
                     /translation="MAHKKGQGSSRNGRDSNAQRLGVKRFGGENVLAGNILVRQRGTK
                     FHPGTNVKIGGDDTLFAVADGLVKFETKRGNRQFVSVHPVK"
     gene            complement(489..797)
                     /gene="rplU"
                     /locus_tag="VF870_10310"
     CDS             complement(489..797)
                     /gene="rplU"
                     /locus_tag="VF870_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX9252626.1"
                     /translation="MFAVVDILGQQFKVSENTKYYVPRLNQEPESEVTFDQVLLLSDG
                     KETKVGSPVVKGAKVTAKVLSHLKDDKVLVFKKKRRKGYRKLNGHRQQLTRIEVTQIG
                     "
     gene            complement(893..>1573)
                     /locus_tag="VF870_10315"
     CDS             complement(893..>1573)
                     /locus_tag="VF870_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9252627.1"
                     /translation="LQRMRSQFLANVSHELRTPIFSIQGYLETLLNGAIDDQKVNKHF
                     LEKANQNTVNLSNLLNDLIDISMIESGEMRMSYRYFDINVFIKSFLSEFTPMANEKNI
                     QLIFSPAKEGLQVFGDKEKIRQVFVNLLQNAIKYTDEGSIEILLNEEKKFVNIAIKDT
                     GIGIPEEDLTRVFERFYRVDKARSRAVGGTGLGLAIVKHIIEAHNSKIMVQSKPGEGS
                     IFSFKLKK"
BASE COUNT          472 a          309 c          219 g          573 t
ORIGIN      
        1 aaaattattg agtttgaatt aaatgcagaa gaaagagcat tacttgataa ttccgcaaaa
       61 gctgtaaaag atttagttgc agatatggaa agactaggat tctaaaatcc tctttcattt
      121 ttattacaaa ggcgattcaa acgagtcgcc ttttctttaa acaaaaaacc cgatccattt
      181 ctgaatcggg tttttactaa attattttgt taaaaattat ttaacaggat gaacacttac
      241 aaattgtcta ttgccgcgtt tggtttcaaa ttttaccaat ccatcagcaa ctgcaaacaa
      301 agtatcatca ccgccaattt ttacgttggt tcccggatga aattttgttc ctctttgtct
      361 aactaaaatg ttaccggcaa gaacgttttc accgccaaat cttttaacac caaggcgctg
      421 tgcattacta tctctaccgt tacgtgacga accttgacct tttttatgtg ccattattaa
      481 atctcctttt atcctatctg ggtaacttca attcttgtta actgctgtct gtgtccgtta
      541 agttttctgt agccttttct tcttttcttt ttgaagacta aaactttatc atcttttaaa
      601 tgtgaaagaa ccttagcggt tacttttgca cctttaacaa ctggactacc gacttttgtt
      661 tctttaccat cacttaataa taaaacctga tcgaatgtaa cttctgattc cggttcctga
      721 ttcaaacgtg gtacgtaata ttttgtattt tcggaaactt taaactgttg tcctaaaata
      781 tctacaactg caaacatttt ttcctcataa attctaattt gagcgtgtaa aactaataaa
      841 acaaaggttt tatgtcaaat ttttaggcag attattttat tgtaaaaacc ggctatttct
      901 tcaatttaaa agaaaaaatg cttccctcac ctggcttgct ttgcaccata atctttgaat
      961 tatgcgcctc aatgatgtgt ttaacgatag ccagccctaa tccggtaccg ccaaccgctc
     1021 tggatcttgc tttatcaaca cgataaaatc tttcaaaaac gcgggttaaa tcttcttcag
     1081 gtatcccgat tccagtatcc ttaattgcaa tatttacaaa tttcttttct tcattcagca
     1141 aaatctcaat gcttccttca tctgtgtact ttattgcatt ttgcaagaga ttaacaaaaa
     1201 cctgtctgat tttttcttta tcaccaaaaa cctgcagtcc ttctttggct ggactgaaaa
     1261 ttaactgaat gtttttttca ttagccattg gtgtgaattc tgaaagaaaa ctttttataa
     1321 aaacattgat atcaaagtat ctgtaactca tccgcatctc accggattca atcatcgaaa
     1381 tatcaataag atcattcaat aagttgctta gatttaccgt attctgattt gccttctcta
     1441 aaaaatgctt atttactttt tggtcatcga ttgcaccatt taaaagagtt tccagataac
     1501 cctgtattga aaaaattggt gttcttaatt cgtgtgatac atttgcaaga aactgacttc
     1561 tcattctttg taa
//
LOCUS       DASWBY010000569         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_701575, whole genome shotgun sequence.
ACCESSION   DASWBY010000569 DASWBY010000000
VERSION     DASWBY010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701575"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..305)
                     /locus_tag="VF870_10320"
     CDS             complement(114..305)
                     /locus_tag="VF870_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252628.1"
                     /translation="MEYNWYKYFNYGSLILAVILVILLVMDAVPKNLYIPLLVVMVII
                     FITRIVARIYFMKQSKKQS"
     gene            complement(381..758)
                     /gene="acpS"
                     /locus_tag="VF870_10325"
     CDS             complement(381..758)
                     /gene="acpS"
                     /locus_tag="VF870_10325"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX9252629.1"
                     /translation="MVIGIGIDIIEIERIKISIDKFGDSFLNKIYTKNELEYCLAKHN
                     KYQHLAARFAAKEAIYKALATEWEKGTTWKSIEITNEPNGLPVVAFFGKLKDFLSEDK
                     DIKISLSHSEHYVTGVAIIFKKY"
     gene            complement(771..2468)
                     /gene="ggt"
                     /locus_tag="VF870_10330"
     CDS             complement(771..2468)
                     /gene="ggt"
                     /locus_tag="VF870_10330"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX9252630.1"
                     /translation="MQTFSKIILFLFISILVSHNSFAESKDPVRGKNGMVVSASELAS
                     RVGVEILKKGGNAVDAAVAVGFALAVTYPAAGNIGGGGFMVIHLNDGTNTSIDYREKA
                     PQSANEFLYQDSLGNFLPEKSQFGVTSSGVPGSVAGLIYALKKYGTLTLTEVIQPAID
                     LARNGFILDYHLAKSLEYEIEYFYKYESSKKIFTKDGNPFAEGELFMQPDLANTLEQI
                     KKKGVDGFYSGVTAGLIVNQIKKQGGYITLEDLMNYKPIERSTIAIEYKGCKVITMGP
                     PSGGGVSLLQMLNILENINFEKNEWGSSLYYHNLIEAMKFSFADRSKHLGDPDYYNVP
                     VDWLISKKYAEVLFDKIKDEATPSSEILPGEVTANSESEETTHYSIIDKFGNAVSTTT
                     TLNSSFGSKVVVEGAGFLLNNEMDDFSSKPGEPNQFGLIGSEANKIEPGKRMLSSMSP
                     TIILKDDKAFMLVGSPGGSTIPTVVLQVILNILDFGMDIQQAIDMPRIHHQWLPDEVN
                     YERYGLSLDVIQALQKKGHVFGGIRTLGRCEGIVVDFENEIIYGATDPRGFGAAIGF"
     gene            complement(2452..>2966)
                     /locus_tag="VF870_10335"
     CDS             complement(2452..>2966)
                     /locus_tag="VF870_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9252631.1"
                     /translation="IPSVMSLFGSVQDDLVTVLKDVKVSLHSLNKYLTDDKLSRDVKQ
                     SISNLNTLTEKLNLMIAENRNDVRSLTKNAVELTETSNNLLKSNKENIDHLFVDLKNV
                     IQKSDTLLTDLNKLTSETMNQQNNVGKLLYDENLINDLKQTLKQVNELTSILIDQLKN
                     DGVNVDANIF"
BASE COUNT          933 a          561 c          425 g         1047 t
ORIGIN      
        1 tacagccttg cggatattgc aaactgaaag tggttcgtag ctttgattta aaaccagaac
       61 tttagaattg agtttaccat ttgtgggaat agatcgttcg aagacttaca cctttaactc
      121 tgcttttttg attgtttcat aaaataaatc cgtgcaacta ttctggtaat aaaaattatc
      181 accattacaa ctaatagtgg aatatacaag ttttttggaa ccgcatccat aacaagtaga
      241 ataactaata ttactgctaa tatcagcgat ccgtaattaa aatatttata ccaattatat
      301 tccataagtc ttttttgatt tttataaaat tactgatttg atttatgatt ttcaattaac
      361 tttttaacgc aaataagctt ttaatatttc ttaaaaatga tagcaactcc agttacatag
      421 tgttctgaat gactgagcga aattttaata tccttatcct cggataaaaa atctttaagc
      481 ttgccaaaaa atgcaacaac aggtaggcca ttgggttcat ttgtaatctc aatacttttc
      541 caggttgtac ccttctccca ttctgtagca agtgctttat agatagcttc cttagctgca
      601 aaccttgctg caagatgctg atacttattg tgctttgcaa ggcagtattc caattcattc
      661 tttgtataga ttttatttag aaatgagtcg ccgaatttat ctatactgat tttaatccgc
      721 tcaatttcaa ttatgtcaat tccaattcca attaccatta cttttgtcct ttaaaatcca
      781 attgcagcac caaaaccacg aggatctgta gcgccataga ttatttcatt ttcaaaatcg
      841 accactattc cttcacatct tcctaatgtt ctaattccac caaaaacgtg tccttttttt
      901 tgtaaggctt gaataacatc caaacttaat ccataccttt cataatttac ttcatctggc
      961 agccattgat gatgaatacg aggcatatct attgcctgct gaatatccat accaaaatcc
     1021 aatatattta gaataacctg aagtacaact gtaggaatgg tcgatccacc gggtgaacca
     1081 actagcataa atgctttatc atcttttaaa atgatagttg gggacataga acttaacatt
     1141 ctctttcccg gttcaatttt gttagcctca ctgcctatta atccaaattg attaggttct
     1201 ccgggctttg aactaaaatc atccatttca ttatttaata aaaatccggc accttcaact
     1261 actactttag acccaaatga agagtttaaa gtcgtggtag tgctaacagc atttccaaat
     1321 ttgtcaataa tggaataatg agtagtttct tcactttctg aattagctgt cacttcacct
     1381 ggtaatattt ctgatgatgg agtagcttca tctttaatct tgtcgaataa gacttctgca
     1441 tatttttttg atataagcca atcaactgga acgttgtaat aatcaggatc accgaggtgt
     1501 tttgatctat cagcaaaaga aaatttcatc gcttcaatca agttatgata ataaaggctg
     1561 ctgccccact catttttttc aaaatttata ttttctaaaa tattcaacat ctgaagaagt
     1621 gaaactccac cgccgcttgg aggacccatt gtaataactt tgcagccctt gtattctatt
     1681 gcaatagttg atcgttctat aggtttgtaa ttcatcaaat cttctagagt tatatatccg
     1741 ccctgctttt tgatttggtt tacgattaac ccagcagtta caccggaata aaatccatcc
     1801 acaccctttt tcttaatctg ttctaaagtg tttgctaaat ccggctgcat aaaaagctca
     1861 ccctcagcaa aaggatttcc atctttagta aatatttttt ttgatgactc atacttataa
     1921 aaatattcaa tttcatattc taaagatttt gccagatgat aatcaagaat aaatccattt
     1981 cgtgctaaat caatcgctgg ctgtataact tcagttaagg ttaacgttcc atattttttt
     2041 aatgcataaa ttaaaccggc tacactacct ggcactccgg atgaagttac accaaactga
     2101 cttttttcag gaaggaaatt tcctaatgaa tcctgatata aaaactcatt tgctgattga
     2161 ggtgcctttt cacgataatc aattgatgtg ttagttccat cattaagatg tataaccata
     2221 aatccgccac cgcctatgtt tcccgcagct ggataagtta cagctaatgc aaaaccaaca
     2281 gccacagcag catcaacggc attaccgcct ttctttagta tttcaacacc tactctggag
     2341 gctaattcac tagcagaaac aaccattcca ttttttccac gcaccggatc ttttgattct
     2401 gcgaacgaat tgtgtgaaac aagaattgat atgaacagaa ataggataat tttagaaaat
     2461 gtttgcatct acatttacac catcattctt aagctgatct ataagtattg aagtgagctc
     2521 attaacttgt ttaagagttt gtttaagatc atttattaaa ttttcatcat acaataattt
     2581 accaacatta ttttgttgat tcatagtctc actggtaagc ttattcaggt ctgtaagtaa
     2641 agtatcgctt ttttgaataa cattttttag atctacaaac agatgatcta tattttcctt
     2701 attactttta agaagattat tgcttgtttc ggttagctca acagcatttt tagttaatga
     2761 tcgcacatca tttctatttt cagcaatcat caaatttagt ttttctgtaa gtgtattaag
     2821 atttgatata gactgtttta catctctact taatttatca tctgtaagat atttgtttaa
     2881 agagtggaga gaaactttaa catcctttag tactgtaaca agatcatcct gtacagatcc
     2941 aaataaagac attactgaag gtatat
//
LOCUS       DASWBY010000570         4440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_97811, whole genome shotgun sequence.
ACCESSION   DASWBY010000570 DASWBY010000000
VERSION     DASWBY010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97811"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..444)
                     /locus_tag="VF870_10340"
     CDS             complement(13..444)
                     /locus_tag="VF870_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9252632.1"
                     /translation="MTVFFSKKCEYGLQAVLFMAASENKIVYPAEEISRKLKIPKEFI
                     SKILQSLTESGIIKSKKGKSGGFKLAKRPSRIKLIDIVDAIDGLESFNSCVLGFPECS
                     PDKPCPVHYQWGSLRTKAYEMLSSETLDKFKEKTLNKINNV"
     gene            complement(511..825)
                     /locus_tag="VF870_10345"
     CDS             complement(511..825)
                     /locus_tag="VF870_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9252633.1"
                     /translation="MNELINKELLVDAINYQNGSIVSKQIIKKPNGNITLFAFDKDES
                     LTEHTSPYEAVVYMVDGEMEIKIDGKPYNVKAGEIIVMPPNVPHGLKAAIKSKMLLTM
                     IK"
     gene            complement(837..3065)
                     /locus_tag="VF870_10350"
     CDS             complement(837..3065)
                     /locus_tag="VF870_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEX9252634.1"
                     /translation="MKKYWIAFSAVIIISFTVLSWVGVKIYQQKPPVPEKVVTDSGVL
                     VFTKSDIQDGQNIWQAMGGMESGSVWGHGSYVAPDWTADWLHREAVVVLNIWARGQYQ
                     SDFDNLSSEQQSSLKARLTEIYKTNTYNNKTGIITIDDARLVAIKSNTEHYSDVFKNG
                     KDEYAIPKNTLSDTEKLRKLNAFFFWSSWAASTNRPNSDVTYTNNWPHEELIKNIPTP
                     DTIVWTGVSIIMLLLGIGGMAWYYAANRGEVEHNKFPSDDPLLGAVVTPSQKAVVKYF
                     WIVSALFILQIILGVITAHYGVEGGAFYGIPLAEIFPYVITRTWHTQLGIFWIATAWL
                     AAGLYIAPAVSGVEPKGQKLGVNILFVALLIVVLGSMVGEWLSIKHLLPDQFWFYFGH
                     SGYEYIDLGRFFQIALLIGLFLWLFLMWRAIRPALKKNDEQKPILTLFLISTIAIASF
                     YAAALMYGKHTNLAIAEYWRWWVVHLWVEGFFEVFATVVIAFLFAKLNLISIKTAGQA
                     TVLSATIFLSGGIIGTLHHLYFSGTPMIVLSLGSVFSALEVVPLMFVGYEAWENIKLS
                     RATEWVKKYKWAINFFVAVAFWNMLGAGLFGFMINPPIALYYMQGLNTTPLHGHAALF
                     GVYGMLGIGLMLFSLRGIRPDIEWNEKPIKWSFWMINFGLFAMVTFSLLPVGLMQTWA
                     SVEHGYWYARSSEFLQTDLMQTLRWLRAFGDTIFAFGALILVYFIFQLSLKFKKEKSN
                     KG"
     gene            complement(3167..3526)
                     /locus_tag="VF870_10355"
     CDS             complement(3167..3526)
                     /locus_tag="VF870_10355"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX9252635.1"
                     /translation="MIKLKRVYDKYSDEDGFRILVDRLWPRGLTKKDAKIDIWLKEIA
                     PSNELRKWYHQNSDEWTTFRKKYSNEIKNKKELLKQIKLLEKENGTISLVYAAKNLNK
                     NNASALLSVLNPKTLNE"
     gene            complement(3544..4215)
                     /locus_tag="VF870_10360"
     CDS             complement(3544..4215)
                     /locus_tag="VF870_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9252636.1"
                     /translation="MKEFRVKVLRIDNVTHDVLCIKVQKPDGFKFTPGQATDVAINKP
                     GYVDQKRSFTFTALNNWDYLEFTIKIYDDHNGVTKEIGKLNVGDQLIIGEPWGSIKYK
                     GEGTFIAGGAGITPFIAILRELDLKNQKGNNKLIFANKTETDIILKDDFVKMLGKNFI
                     NILSLEKNDKYNYGYITEEFLKLNVDSLDKMFYLCGPPAMMDVVEKILANLNVDKDLI
                     VKEEF"
BASE COUNT         1405 a          881 c          648 g         1506 t
ORIGIN      
        1 cattaaaaac tattatacat tatttatttt atttaaagtt ttttctttga acttatctaa
       61 agtttcagag cttaacattt cataagcttt ggttctcaaa gaaccccact ggtaatgaac
      121 cgggcacggt ttatccggag agcattccgg aaaaccaagt acacaggaat taaaactttc
      181 caatccatca attgcatcca ctatatcaat tagttttatc ctggatggcc gctttgcaag
      241 tttaaatcca cccgatttgc ctttctttga tttaataatt ccgctttccg tcaggctttg
      301 aagaatcttc gaaataaact cttttggtat ttttaacttt ctagagattt cttcagccgg
      361 gtaaactatc ttattctcac tagcagccat aaaaagaact gcttgcaaac catactcgca
      421 cttttttgag aaaaaaactg tcatattcta aatcagatta aattgtctga ttaaagatgt
      481 caaaaataaa atattttgtc aatacatttt ctactttatc atcgttaaaa gcattttgga
      541 tttaattgca gcttttaatc catgaggcac attcggaggc ataacaatta tttctcctgc
      601 tttaacattg tatggctttc cgtcaatctt aatttccatt tcaccatcaa ccatatacac
      661 aaccgcttca taaggtgaag tgtgttctgt taatgattca tctttatcaa aagcaaaaag
      721 agtaatgttt ccgtttggtt ttttaattat ctgtttgctt actatagaac cgttctgata
      781 atttattgca tcaacaagaa gttctttatt aattaattca ttcattaata tcctgcctat
      841 cctttattag atttttcttt tttaaacttt aacgacagtt gaaatatgaa gtatacaaga
      901 atcaatgctc cgaatgcaaa tattgtgtca ccgaatgctc ttaaccatcg cagcgtctgc
      961 attaaatctg tttgcaagaa ttctgagctt cttgcatacc agtagccatg ttctacggaa
     1021 gcccaggtct gcatcaatcc caccggaagc agactgaatg taaccattgc gaacaaacca
     1081 aaatttatca tccagaatga ccatttaatt ggtttttcat tccattcaat atccggtctt
     1141 attccgcgca gtgaaaacag cattaatcca attccaagca ttccataaac accaaaaagc
     1201 gccgcatgcc cgtgaagagg tgttgtattt aatccttgca tataataaag tgcaatcggc
     1261 ggattgatca taaatccgaa taatcctgca ccaagcatat tccagaaagc aacggcaaca
     1321 aaaaagttta ttgcccattt atattttttt acccattctg tagcacgtga aagttttatg
     1381 ttttcccagg cttcataacc gacaaacatt aatggaacta cttcaagtgc actaaaaaca
     1441 gagccaagtg aaagtacaat cattggtgta ccggagaaat acaaatgatg cagtgtacct
     1501 attattccgc cggataaaaa tatagttgca gatagaacag ttgcctgccc tgccgttttg
     1561 atagatatca aatttaactt tgcaaatagg aatgcaatta caacagttgc aaaaacctca
     1621 aaaaatcctt ccacccaaag atgcactacc caccatctcc agtattctgc tatagcaaga
     1681 tttgtgtgtt ttccatacat caacgcagca gcataaaatg atgcaattgc aatagtggaa
     1741 ataaggaata aagttaaaat aggtttctgc tcatcatttt tctttagtgc cggtcgaatt
     1801 gcgcgccaca ttaaaaacaa ccaaagaaat aatccgataa gtaaggcgat ctgaaaaaat
     1861 cttccaagat caatatactc ataaccgctg tgcccaaaat aaaaccaaaa ctgatcaggc
     1921 aataaatgct ttatacttaa ccattcgcct accattgaac caagtacgac aattaagaga
     1981 gctacaaaaa gaatattgac cccgagcttt tgtccttttg gctcaactcc acttacagca
     2041 ggggctatat ataaaccagc cgcaagccag gcagttgcta tccaaaatat tcccaactga
     2101 gtgtgccagg ttcttgtaat tacataaggg aaaatttctg caagcgggat tccgtaaaag
     2161 gcacctcctt ctacaccgta atgagcagta attacaccaa gaattatttg cagtataaac
     2221 aatgcagaca caatccaaaa atattttacc acagcctttt gagaaggtgt taccactgcg
     2281 ccaagtaaag gatcatctga tggaaattta ttatgctcaa cttcacctct gtttgcggca
     2341 taataccagg ccatcccacc aatacccaaa agcagcatta taatactcac accggtccaa
     2401 acaatagtat cgggcgtagg tatgttttta attaattctt cgtggggcca attatttgtg
     2461 taggttacat cactatttgg tctgtttgtc gatgcagccc aggaactcca gaaaaagaat
     2521 gcattaagtt ttctaagttt ttcagtatcg cttagcgtgt ttttcggaat tgcatattca
     2581 tcttttccat ttttaaaaac atcggagtag tgttcggtat tagatttgat tgctacaagt
     2641 cttgcatcat caatagtaat aatacctgtt ttattattat aagtattagt tttataaatt
     2701 tctgttaatc tggcctttag tgatgattgc tgttcagaag aaagattatc aaagtctgat
     2761 tggtactgcc cccttgccca aatatttaat acaacaactg cttcccggtg aagccagtca
     2821 gcagtccagt caggagctac ataactgccg tgtccccaaa cagaacctga ttccattccg
     2881 cccatagcct gccagatatt ttgtccatcc tggatatcag attttgtaaa aaccaatact
     2941 ccgctatcgg taacaacctt ttcgggcaca ggaggttttt gctgataaat ttttactccc
     3001 acccagctta aaacagtaaa tgagataatt atgactgctg agaatgctat ccaatatttt
     3061 ttcatagtaa ttcctttatt tacttaacta taataaagcg attgaatttt tttattgatc
     3121 aaaatctaga tgctttttaa gataaattcc ttgacttaag tcagaactac tcatttaaag
     3181 ttttaggatt tagaaccgaa agaagtgcag atgcgttatt tttgttcaga ttttttgcag
     3241 cataaacaag actgatagtt ccattttctt tttcaagaag ctttatttgt tttagcaatt
     3301 cctttttgtt ctttatctca ttagaatatt tttttctaaa tgtagtccac tcatcggaat
     3361 tctgatgata ccattttcta agttcattac ttggagcaat ttcttttaac catatatcta
     3421 tttttgcatc tttcttggtc aatcctcttg gccatagtct gtcaacaagg attctaaaac
     3481 catcttcatc agaatattta tcatatactc ttttaagttt tatcataata ctgattacaa
     3541 ttattaaaat tcttccttca caattaaatc tttgtctaca ttcaagtttg ccagtatttt
     3601 ttcaactaca tccatcatag cgggaggccc acagagataa aacatcttat caagactatc
     3661 tacattcaat tttaagaatt cttctgttat gtatccataa ttatatttat cattcttttc
     3721 aagagaaagg atattaataa aattctttcc cagcattttc acaaaatcat cttttaagat
     3781 tatatctgtt tcagttttgt ttgcaaatat tagtttatta tttccctttt gattttttaa
     3841 atcaagttca cgcagaatag caataaaagg agttatgcct gcgcctccgg caataaaagt
     3901 cccctcgcct ttatatttga ttgaacccca tggttctccg ataattagct gatcaccaac
     3961 attaagtttt ccaatttcct ttgtaactcc attatgatca tcataaattt taatcgtgaa
     4021 ttcaaggtaa tcccagttgt ttagtgcagt aaaagtaaaa gatctttttt gatcaacata
     4081 gccaggctta ttaatggcaa catcagttgc ctgccctggt gtaaatttaa acccatcagg
     4141 tttttgaact ttaatacaca atacatcatg tgtaacatta tctattcgca ggacttttac
     4201 tctgaactct ttcatatttt acctctattt aaattgatta tcgatagtat cgttttttct
     4261 taatataaaa atcaatatat cattttaata gactttaata gttatagaag tttgtatttg
     4321 cttgaaatgc atccattgga caaatttcta cacatttaat cacttcaaat cctgcgcact
     4381 cattgcaaat cttttctaaa ataaaaaaat gttctgttga aattgcagga taattctttt
//
LOCUS       DASWBY010000571         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_359811, whole genome shotgun sequence.
ACCESSION   DASWBY010000571 DASWBY010000000
VERSION     DASWBY010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359811"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VF870_10365"
     CDS             <1..1069
                     /locus_tag="VF870_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX9252637.1"
                     /translation="YFAQNVVAAIVATIVMIIAGFFFAAVSGYLVGIIGSSNNPISGL
                     TLSTLVVAAILMVALGMKGMQGVAAVLGVAAVVCVAAAVAGEMLQDLKAGHILGGTPW
                     KMQLGDLIGVVVAASIMFIPLLVLHEGDIADKVNKQLIKIENNHQMVIDYQGSKEEFK
                     GKQYSIEDFKKLDKSAQKEFLDLNAGFGGDALPAPQASLMAILSKGIVAGDMEWILIF
                     TGMLMGVGFIMMNVKSPMLVSVGMYLPLGTTFAIFVGGLIKGIVEKVNAKKNFNESQK
                     SRVENNGILLAAGLIAGEALIGLLFAGFAFFEVQLFSFFAEPSFLVSLIVFAFIAWLL
                     VQIPVKNAGKPDDPAPPQVSM"
     gene            1248..1619
                     /locus_tag="VF870_10370"
     CDS             1248..1619
                     /locus_tag="VF870_10370"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX9252638.1"
                     /translation="MALSFKERKKILKSVNTLDLTPIKLYSDEQNEDKLVTVIIPKFK
                     NKIVVKLVSPKLKSDHFKVKLDKFGSAVWLKINGKTKVDQIIKDLKQKFGDEIQEENE
                     RTTKFIFQLYEQGFISFKEIN"
     gene            1632..>2678
                     /gene="pepD"
                     /locus_tag="VF870_10375"
     CDS             1632..>2678
                     /gene="pepD"
                     /locus_tag="VF870_10375"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HEX9252639.1"
                     /translation="MGSVLGHLKPELVWYHFEEICKYPRPSKKEEKIAEYVVSVGKRL
                     GLQTEKDKFGNIVIRKPATPGKENLKTVVLQGHIDMVCEKNRDVEHDFDNDPIYPYID
                     GDWVKAKGTTLGADNGIGVASALAILEDKNVEHGPIECLFTLDEETGLTGASSLKKNW
                     LKADILINMDSEELGTIFIGCSGGKNTVATFKAKSEKSPRNYSSFELKVAGLKGGHSG
                     LEIHVGRGNAVKILNRAIWSYAQENKIRLSEINGGNKHNAIPREAFAVVLVPKNDDKK
                     FKKFVAKYNETVKTELAAVEPDLVVSIEKHSTPEKVMDEKTQRHLVNALYAVPHGVIK
                     MSNDIPGLVETSTNL"
BASE COUNT          888 a          398 c          559 g          833 t
ORIGIN      
        1 ttatttcgca caaaatgttg ttgcggcaat agttgcaaca atagttatga ttattgctgg
       61 atttttcttt gccgcagttt ccggttactt agttggaatc ataggttcct caaataatcc
      121 tataagcgga ctcacattat caactttagt tgttgctgca attttaatgg tggcacttgg
      181 aatgaaagga atgcaaggtg ttgctgcagt tcttggtgtt gccgcagtcg tttgtgttgc
      241 tgctgctgtt gccggtgaga tgctgcagga tttaaaagca ggacatattc ttggtggtac
      301 tccctggaaa atgcagcttg gtgatcttat tggtgttgtt gttgccgctt ctattatgtt
      361 tattccttta ttagttctcc acgaaggaga tatcgctgat aaggtaaata aacaattgat
      421 caaaattgaa aacaatcatc aaatggtcat tgattaccag ggttcaaaag aagaatttaa
      481 aggtaaacaa tactctatcg aagattttaa aaaattagat aaatcagcac agaaagaatt
      541 tcttgatttg aacgcaggat ttggcggcga tgcattacca gcaccgcagg caagtttgat
      601 ggcaattcta tcaaaaggaa ttgttgccgg tgatatggaa tggattttaa tttttaccgg
      661 aatgctgatg ggtgtgggct ttataatgat gaatgtcaaa agtccaatgc tagtcagtgt
      721 tggaatgtac cttccgttag gtaccacctt tgcaattttt gttggcggtt taataaaagg
      781 aattgtcgaa aaagttaatg caaagaaaaa ctttaatgag tctcagaaat cgagagtgga
      841 aaataatgga attttactag ctgcaggatt gatcgctggt gaggcactca ttggtcttct
      901 tttcgccggt tttgcatttt ttgaagttca gctgtttagt ttctttgctg aaccatcgtt
      961 cttagttagt ttaattgtat ttgcatttat cgcgtggctg cttgttcaga ttccggttaa
     1021 gaatgccggt aaacctgatg atcctgcacc accgcaagta tcaatgtaat tttttatttg
     1081 gggttagcaa aaatatttat caaggtcatt cccatgcaag tgggaatgac aactaagcca
     1141 tcttgagtga aatcgaaaga tgaaatttct ttagttattt ttattaagtg tcacatcgac
     1201 tttgctcagt gtgacactga ttattttgag gcaggtacta cacttttatg gcattatcat
     1261 ttaaagaacg aaaaaaaatc ttaaagagtg ttaatacact tgatcttact ccaataaagc
     1321 tatattctga tgagcagaac gaagacaagt tagtaactgt aattattcca aaatttaaga
     1381 ataagattgt tgtaaaatta gtctcaccaa aacttaagag tgatcacttt aaagttaaac
     1441 tagataaatt tggttctgcg gtttggttaa agataaacgg caaaacaaaa gttgatcaga
     1501 taataaaaga tcttaagcaa aaatttggtg atgaaattca ggaagaaaac gaaagaacta
     1561 caaaatttat atttcagctc tacgagcaag gattcatttc atttaaagaa ataaactaaa
     1621 aggagtctat tatgggatca gtattaggtc atttaaaacc agaattggtt tggtatcatt
     1681 tcgaggagat ttgtaaatat cctcgtccat caaaaaaaga agaaaaaatt gcagagtatg
     1741 ttgtatctgt tggaaaacgt ttaggtcttc aaacagaaaa agataagttt ggaaatattg
     1801 ttatccgcaa acctgccaca ccaggaaaag aaaatctaaa aacggttgtg cttcaggggc
     1861 atattgatat ggtttgtgaa aaaaacaggg atgttgaaca cgattttgat aacgacccca
     1921 tttatcctta catagatggt gattgggtaa aagcaaaggg tacaactctt ggcgctgata
     1981 atgggattgg tgttgcaagt gcgcttgcaa ttttggaaga taagaatgtt gagcatggtc
     2041 cgatcgaatg cctctttact cttgatgaag aaacaggttt aaccggtgct tcaagtttaa
     2101 aaaagaactg gctaaaagct gatatactta taaatatgga ttctgaagaa cttggaacta
     2161 tattcattgg atgttccggt ggaaaaaata cagtagctac attcaaagct aagtcagaaa
     2221 aatctccgag aaattattct tcattcgaat tgaaagtcgc tggattaaaa ggcggacact
     2281 caggtcttga aatacatgtt ggaagaggta acgcagtaaa aattctgaac agggcaatct
     2341 ggagttatgc acaggaaaat aaaattagac tttcagaaat taatggagga aacaaacaca
     2401 acgctattcc tcgtgaagca tttgcagttg tacttgttcc caaaaatgat gataagaaat
     2461 tcaagaaatt tgttgcaaag tataatgaaa cagttaaaac tgaattagct gctgttgaac
     2521 cggatttagt agtctctatt gagaaacatt caacacctga aaaagttatg gatgagaaaa
     2581 ctcagagaca tttagtaaat gctttgtatg ctgttcctca cggtgttata aaaatgtcaa
     2641 atgatattcc aggattagtt gaaacctcaa ccaactta
//
LOCUS       DASWBY010000572         5550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_173515, whole genome shotgun sequence.
ACCESSION   DASWBY010000572 DASWBY010000000
VERSION     DASWBY010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173515"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..1172)
                     /locus_tag="VF870_10380"
     CDS             complement(357..1172)
                     /locus_tag="VF870_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252640.1"
                     /translation="MKTFIYLITAFIIYCSLIYANGGPPPAPPLFKAKNTGSVYQAPL
                     ITGKNQNIFWARFSYNAVSELDGQIKLEGTYTNTQATENGKNFEWQTPSTVINFGLSA
                     RVAESGAIVLNIGVGKLDSWELAGLEAAYCGLMVNKDHHKARLLLGINIHTTDFDWFT
                     DSTNNSISKSGVEWDPALSIIYNSDFDDWIVNPYVQFSYSCQTLYDSDKEDYSTEVYR
                     NVNVFSCVPGVTYRFNDNIMANLGTAITYVNNIENSKNLVITPQLQFSYYFMP"
     gene            1432..1995
                     /locus_tag="VF870_10385"
     CDS             1432..1995
                     /locus_tag="VF870_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252641.1"
                     /translation="MDPIIHLWLPILVSAVVVFFASFLLHMLVKHHNSDFKQIPDEAR
                     IMDELRKSNIPAGDYVFPYAKDDKERNSKEYKDKYEKGPVAIITVYPSGPMSMGSSLV
                     MWFLYSIVVGVFAAYIAGHALAPGAHYLAVFRFAGCTAFIGYTLALLHDSIWFKRSWA
                     TTFKYIFDGLIYALLTGGVFGWLWPAM"
     gene            complement(2118..3071)
                     /locus_tag="VF870_10390"
     CDS             complement(2118..3071)
                     /locus_tag="VF870_10390"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HEX9252642.1"
                     /translation="MSDKKIIAVLGATGAQGGGLVRSILKDKSGEFAARALTRDVNSD
                     KAKSLANMGAEVFKAELDDFDSLKKAFSGAYSVYGVTNFWEHFSPEKEKAQAKSIANA
                     AKDADVKHIIWSTLEDTRNFIPLTDNRMPTLGNGQYKVPHFDGKGESNKYFTELNLPV
                     TLLNTSFYWDNFIYFGMGPKKGTDGKLTLSLPIGERKLAGIAAEDIGKSAYGIFKNGS
                     EFIGKTVAITGENLTGKQMADSFAKALGQEVVFNAIPFDVYRGLGFPGADDLGNMFQF
                     YHDFEEYFCGIRSVDFTKSINPELKSFDNWLSEVKSKIPLE"
     gene            complement(3370..5127)
                     /locus_tag="VF870_10395"
     CDS             complement(3370..5127)
                     /locus_tag="VF870_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX9252643.1"
                     /translation="MRAALLILIASFSISFAQNDAWIRINQLGYLPNTPKIAVFISKQ
                     NISVKEFSIREIESDRIVFTSDKIKSFGNKWNFKSTVQLDFSSFNTEGSFYIEYSNVR
                     SPLIRISENVYNGTADFLLKYMRQQRCGYNPFLKDSCHTHDGFIIYHPELDSTYIDVK
                     GGWHDASDYLQYVTTSANTVYQMLFAYQNNPRAFKDEYDANGNPGPNGIPDILDEVKW
                     GIDWLLKMNPEKDIMFNQIADDRDHMGFRLPNKDTISYGKGLERPVYFATGKPQGIFK
                     YQNRTTGLASTAGKFSSAFALASVLFKEHYPELCTKLLEKSIEAYELGKKFPGVCQTA
                     PCRAPYFYEEENYKDDMELAASQLYQITKSKPYLTDAINFGNDEKIIAWMGADTAKHY
                     EWYPFINLGHYLLANEGSNDEKEKFTIYLKQGIEKVISRGNDNPFNIGVPFIWCSNNL
                     VAAFLSQCRLYNQLTGDDQFLYGEAALRDWLLGCNPWGTSMIVGLPADGNYPSDPHSA
                     FTHLDNYDINGGLVDGPVYNTIYSKLIGITLYSPDEYAEFQNDYVVYHDDYGDYATNE
                     PTMDGTASLTYYLSSLEKK"
     gene            complement(5143..>5550)
                     /locus_tag="VF870_10400"
     CDS             complement(5143..>5550)
                     /locus_tag="VF870_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252644.1"
                     /translation="EAGAAFNELTRSNKDDLLVRQIKNAWPNSFRASRFIPAVEYINA
                     SRVRSLLIQQMQKLMSTIDLYIAPTNEGDSNLLTNLTGHPCVVLPNGFSDMGTPTSIT
                     FIGRLFDEGRLIAFAKLYQDATGHHTKHPEMFK"
BASE COUNT         1761 a          976 c          949 g         1864 t
ORIGIN      
        1 ttcccagtca tccccggcag gagaatttaa agaatagttt ttcattttta tttgcttttc
       61 tgattcaata ctaacagcgt ttgaaccgga gctgcaagca caaagaagaa taaataagct
      121 gacaaggata attgtgagaa ttagtctttt catttcacac ctctcaaaaa atttcaatta
      181 aattataact aaaaacccaa gtctaggtgt catgaaattg taaaaattta taaaagaaat
      241 ttttagcaaa aagaataatt attgtactga cttttttatc cggcatcact cagaaaattc
      301 gataatcagg ccacatgctt gtcttaagca attatgtaat attacacaac ctacacttat
      361 ggcataaagt aataactgaa ctgaagttgc ggtgttataa caagattttt cgagttttct
      421 atattattaa cataagttat tgcagtgcca agatttgcca taatgttatc attaaaccta
      481 tatgttacac ccgggacaca gctaaataca tttacatttc tatagacttc tgtgctgtaa
      541 tcttccttat cagaatcgta taaggtctgg caggaataag aaaactgtac ataaggatta
      601 acaatccaat catcaaagtc ggagttataa ataatgctga gcgcaggatc ccattctacg
      661 ccgcttttag aaatgctatt atttgttgaa tcagtaaacc agtcaaaatc cgttgtatga
      721 atattaattc caagtaataa tctggcctta tgatgatctt tattaaccat taatccacaa
      781 tacgcagctt caagacctgc aagttcccag ctatctagct tacctacgcc aatattaagt
      841 acaattgcac cgctttctgc cactcttgct gaaagaccaa aattgattac agttgaagga
      901 gtctgccatt caaagttttt accattttcc gtcgcttgtg tgtttgtgta agttccttca
      961 agtttaattt gcccatcaag ttcagaaaca gcattatatg aaaaacgagc ccaaaaaata
     1021 ttttggttct tgcctgtaat tagcggggcc tgataaacag aacctgtatt tttagcttta
     1081 aatagaggtg gagctggggg cggacctcca ttggcataaa ttaaagagca ataaataata
     1141 aatgctgtta tcagatatat gaatgttttc atttttgtat ctcctttaaa atctgatata
     1201 aaattacact acataattaa gtcagttgtc atgattttgt aaaaaataaa acttttggat
     1261 gattttgtgc aaagaatgac ttggggattt atagtgtgat attgaatgcc cctttagtta
     1321 aatgctataa gaggataaaa gtgcaaaaat gttttgcaat tcttaaaaag aaataactat
     1381 tttttaccgg gcataaattc aaaaatattt tattaataaa aaggagacga tatggatcca
     1441 ataattcatt tgtggcttcc gatacttgtt tctgctgttg ttgtattctt tgcaagcttc
     1501 ttacttcata tgctggttaa acatcataat agtgatttta aacaaatacc tgatgaagca
     1561 agaataatgg atgaattaag aaagtcgaat attcctgccg gggattatgt ttttccatat
     1621 gcaaaagatg ataaagaaag aaactcaaag gagtataagg ataagtatga aaaaggtccc
     1681 gttgcaatta taactgttta tccatcagga ccaatgagta tgggttcaag tcttgtgatg
     1741 tggtttctgt attcaatagt agttggagta tttgcagcat atattgcggg acatgctctc
     1801 gcacccggcg cacattattt agcagtattc cgatttgcag gttgtacagc ttttattggc
     1861 tacacacttg cgcttttaca tgacagcatc tggtttaaaa gaagctgggc aacaacattt
     1921 aaatatattt ttgatggttt gatttatgca ttacttaccg gtggagtgtt tggctggtta
     1981 tggcccgcta tgtaaagaaa tatttaaaga ttaaatattg gatcgtaaga agcaataaaa
     2041 ttaaagacaa aaaaaagaca cggaacaaaa tctccgtgtc ttttattata atataattta
     2101 gaaaatcaaa ataaaattta ttcaagtgga attttagatt tgacttcact taaccaatta
     2161 tcaaagcttt taagttctgg attaattgat tttgtaaaat cgacacttct tataccgcaa
     2221 aaatactctt caaaatcatg ataaaactgg aacatattgc caagatcatc agcaccggga
     2281 aaacctaatc ccctgtaaac atcaaatgga attgcgttaa aaacaacttc ctgccccaat
     2341 gctttagcaa aagaatcagc catctgttta cctgtaagat tttctcctgt tatagctaca
     2401 gttttaccga taaactcact gccattttta aaaataccat aggcagattt accaatatct
     2461 tcagcggcaa tgcctgcaag ttttctctct ccaatcggaa gtgaaagtgt aagttttccg
     2521 tcagttcctt ttttcggtcc cattccaaaa taaataaaat tatcccaata aaaagaagtg
     2581 tttagcaatg ttaccggtaa atttagttct gtaaaatatt tattggattc acccttgccg
     2641 tcaaagtgag gcactttgta ttggccatta ccaagcgtgg gcattctgtt atctgtaagc
     2701 ggaataaaat tgcgtgtgtc ttccagggtt gaccaaatta tatgctttac atctgcatct
     2761 ttagcagcat ttgcaataga ttttgcctgg gctttttctt tttcaggtga aaaatgttcc
     2821 cagaagttag ttacaccata gacactataa gcaccgctga atgctttttt tagactatca
     2881 aagtcatcaa gctctgcctt aaatacttct gcgcccatat ttgcgagaga ttttgcttta
     2941 tctgaattta catccctagt taatgctcgt gctgcaaatt cacctgattt atctttaaga
     3001 attgaacgga caagtccacc gccctgtgca cctgttgcac caagtacggc aataattttt
     3061 ttgtctgaca taaagggctt cctgtattaa ttacgtgaat cgtttattta taaaaaccgg
     3121 atttgattgt tgtgtttact tttcagtaag tatagttatt ctactgaatt aagttacttt
     3181 gataattatt aaaaaggaat gttaaaagac ttttagtgac atggaacata gggggtgaaa
     3241 aaaagctacg gtttacttaa aagtccgaat aaactgtttg tattcttcta ttttcattaa
     3301 tgaattacta atttgagttt aattagttta ccaataatga acttttaaga tcttctattt
     3361 caataattat tattttttct ccaatgacga taaataataa gttagacttg ctgttccatc
     3421 cattgttggt tcgttggtag cgtaatctcc gtaatcatcg tgatatacaa cataatcatt
     3481 ttgaaattct gcatattcat ctggactgta taaagtaata ccaataagtt tgctgtaaat
     3541 agtattatag acaggaccat caaccagacc gccatttata tcataattat ctaaatgagt
     3601 aaacgctgaa tgtgggtcag atggataatt tccgtcagcg ggcagaccaa ctatcatact
     3661 tgttccccag ggattgcagc ccaaaagcca atcacgcaag gccgcctctc cataaagaaa
     3721 ttgatcatca cctgttaatt gattataaag tctgcattgt gataaaaatg ccgcaacaag
     3781 attgtttgaa caccagataa atggcactcc aatattaaag ggattgtcat ttcccctgga
     3841 gataactttt tcaattcctt gctttaagta aatggtaaat ttttcttttt catcattgct
     3901 gccttcattt gcaagaagat aatggccaag attaatgaat ggataccatt cataatgctt
     3961 agctgtgtca gcacccatcc aggcgataat tttttcatca ttaccaaaat taatagcatc
     4021 tgttaaataa ggtttacttt ttgtgatttg atatagttgc gatgctgcca gttccatatc
     4081 atctttataa ttttcttctt cataaaaata tggcgcacga cacggtgcgg tttggcaaac
     4141 tcccggaaat tttttaccaa gttcatatgc ctcaattgat ttttctaaaa gttttgtaca
     4201 caattccgga taatgttctt tgaataaaac tgaagcaagc gcaaatgcag aagaaaattt
     4261 tccggcggtt gaggcaagcc ccgttgtgcg attctggtat ttgaagatgc cttgtggttt
     4321 accggttgca aagtaaacgg gtctttctaa tcctttgccg taagaaattg tatctttatt
     4381 aggtaatcta aatcccatat ggtcgcgatc atctgcaatt tgattgaaca tgatatcttt
     4441 ttctggattc atttttaata gccagtctat tccccatttt acttcatcaa gaatatcagg
     4501 gatgccattc ggacctgggt taccattagc atcatactca tctttaaatg ctcgcggatt
     4561 attttgatat gcaaacaaca tttgataaac cgtgtttgca gaagttgtaa catactgcaa
     4621 ataatcagag gcatcgtgcc aaccgccctt tacatcaata tacgttgagt ccagttcagg
     4681 atgataaatt ataaacccat cgtgcgtgtg gcaggaatct ttaagaaaag gattgtaccc
     4741 gcatctttgc tgtcgcatat attttaacaa aaaatctgca gtaccgttat aaacattttc
     4801 agaaattctg atcaacgggg agcgaacatt gctgtattca atataaaaac ttccttcagt
     4861 attaaaggat gagaaatcta actgaactgt tgatttgaaa ttccatttat tgccaaaaga
     4921 tttaatttta tctgaagtaa aaactatcct atctgactct atctctctta ttgaaaattc
     4981 ttttacagaa atattctgct tactaataaa cacggcgatc ttaggcgtat tgggtaaata
     5041 gccgagctga ttgattctga tccatgcatc attctgcgca aaggaaattg aaaaggaagc
     5101 tataagaatt aatagtgcgg ctctcatatc attataaata ttttatttaa acatttccgg
     5161 atgtttagtg tgatgacctg ttgcatcctg atatagcttt gcaaatgcaa ttaatcttcc
     5221 ttcatcaaat agccttccga tgaatgtaat acttgttggt gttcccatat cagaaaaacc
     5281 gtttggaaga acaacgcatg ggtggccagt taaatttgtc agtagattgc tgtcgccttc
     5341 atttgttggt gcaatatata aatctattgt gctcataagt ttttgcattt gctgtataag
     5401 caagcttcta acgcggcttg catttatata ttctactgcg ggaataaatc ttgatgcgcg
     5461 aaatgagttt ggccaggcat ttttaatttg tcttaccagt aaatcatctt tgttgcttct
     5521 tgttaattcg ttaaaggcag cacctgcttc
//
LOCUS       DASWBY010000573         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_26780, whole genome shotgun sequence.
ACCESSION   DASWBY010000573 DASWBY010000000
VERSION     DASWBY010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26780"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VF870_10405"
     CDS             <1..814
                     /locus_tag="VF870_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9252645.1"
                     /translation="MIALFFRLPDNKHPLHDTDYMKSVSADKYGIVIESTDAKFDESS
                     VTEFLKKLNPLKIEVIYYPEEETYPIFQPKFLMFLIVISVVVSGGTYIMLNKLLYITP
                     FNMMMEQDKLNPQHSSELFADGRGMQSPVEGTVARGFIPYPYAGQTNPTEVLANPYLP
                     TKENIELGKAKFFTYCTPCHGYYADGDGRLRGQFPNPPTLHSKRARDFSDGMIYHIIT
                     NGQNVMPSYASQITREERWAIVNYVRVLQRAKNASDADLIEMNKEAGSNVAN"
     gene            801..1991
                     /locus_tag="VF870_10410"
     CDS             801..1991
                     /locus_tag="VF870_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HEX9252646.1"
                     /translation="MSQTDFNYQKKELPAGLSKIGLIFFVVGTVLSLLAFFVDQERAV
                     FNYLVTYMMIVSIGLGSLFIIALEYIAGADWSTPFRRIPEFFAGLLPLLFVLVIPLLV
                     LNHDLFHWAHEEAVAEDKILQGKAPYLNITFFVIRTFVFIGLWSLFYWVMTRNSRKQD
                     QSKDQTLTKKNIRLAAIFIPVFALSLTFTAIDFMMSLEPHWFSTIFGVYFFAGTVVAA
                     LAAITLVIVNLKEKGYFSPWMNDDHLFSLGALLFAFINFWAYIAFSQFLLIWYADLPE
                     ETFWFLTRWNGGWVFFSIGLIMIHFLVPYFALLTQPSKMNPKRLKFIAVWLLFAHLFD
                     LFWLIMPNMGSMKSGYIFSWIDLVFPIAGIGIVMLVFNYKAKKDNLIPIGDPKLKRGI
                     DFHL"
BASE COUNT          623 a          322 c          396 g          703 t
ORIGIN      
        1 aatgatagct ttgtttttca gattgcctga taataaacat ccgctgcacg atacagatta
       61 tatgaaaagt gtttctgctg ataagtatgg tattgtgatt gaatcgaccg atgcgaaatt
      121 tgatgaatca tcagtaacag agtttcttaa gaaattaaac ccattaaaga ttgaagtaat
      181 ctattatcct gaagaagaaa cttatccaat atttcagcca aaatttttaa tgtttctaat
      241 agtaatttca gtagttgttt cgggcggcac atatataatg ttaaataaac ttctatacat
      301 aacacctttt aacatgatga tggaacagga taaacttaat ccgcagcata gcagtgaact
      361 ttttgcagat ggtcgaggta tgcagtcccc ggttgaagga actgttgcaa gaggatttat
      421 accatatcct tatgcggggc agacaaatcc tacggaagtt ttagcaaatc cgtatttacc
      481 gacaaaagaa aatatcgagt taggtaaggc taagttcttt acatattgca ctccctgtca
      541 tggctattat gccgatggtg acggcagact aagaggacaa tttccaaatc cgccgacttt
      601 gcattccaaa cgagcaagag attttagtga cggaatgatt tatcatatta tcactaacgg
      661 acagaacgtt atgccttctt atgcttctca aattacaaga gaagaaagat gggcaatcgt
      721 aaattatgtt agagttttac agcgcgcaaa aaatgctagc gatgctgatt taattgaaat
      781 gaataaggag gctggtagca atgtcgcaaa ctgatttcaa ttaccagaaa aaagaattac
      841 ccgccggttt atcaaaaata ggactaatat tttttgttgt tggaaccgtt ttatctttgc
      901 ttgctttttt tgtggatcag gaaagggcag tgtttaatta tctcgtaact tatatgatga
      961 ttgttagtat cgggctgggt tctttgttca taattgctct ggaatatatt gctggtgctg
     1021 attggagtac cccattcaga agaatacccg aattttttgc agggctatta cccttattgt
     1081 ttgtgcttgt aatcccattg ttagtcctta atcacgatct ctttcactgg gcacatgaag
     1141 aagctgtggc cgaagataaa attttacaag gcaaagcgcc ttatcttaat attacttttt
     1201 ttgttatcag aacttttgta ttcattggat tgtggagctt attctactgg gtgatgacaa
     1261 gaaattccag aaagcaggat caatctaaag atcaaacact tactaaaaag aacattcgat
     1321 tagccgctat ttttattcct gtttttgcat taagtcttac atttactgca atagatttta
     1381 tgatgagcct agagcctcat tggttctcta caatttttgg agtttatttc ttcgccggaa
     1441 ctgttgttgc tgcattggct gcaattacat tagtaatagt taatttaaaa gaaaaaggtt
     1501 acttcagtcc atggatgaat gatgatcatc tttttagttt aggtgcattg ttgtttgcct
     1561 ttataaactt ttgggcttac atagcgtttt ctcaattttt attaatctgg tatgctgatt
     1621 taccggaaga aactttttgg tttttaacca gatggaatgg cggttgggta tttttctcta
     1681 taggattgat catgattcac tttcttgtcc cgtacttcgc tttactcaca cagccatcta
     1741 aaatgaatcc gaaaagatta aaatttattg cagtgtggtt actctttgcc catctatttg
     1801 atttgttctg gttgattatg ccaaatatgg ggtcaatgaa aagtggttat atctttagtt
     1861 ggattgattt agtttttccg attgccggaa ttggaattgt tatgcttgta tttaattata
     1921 aagctaaaaa agataacctg atcccaatcg gtgacccaaa attaaaaaga ggaattgatt
     1981 ttcatctcta atagaaatga gatataaggt taaatatgaa agaaatttta gacggaataa
     2041 aaag
//
LOCUS       DASWBY010000574         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_208505, whole genome shotgun sequence.
ACCESSION   DASWBY010000574 DASWBY010000000
VERSION     DASWBY010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208505"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VF870_10415"
     CDS             complement(<1..1444)
                     /locus_tag="VF870_10415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX9252647.1"
                     /translation="MILKKALKKYFNYDNYRLGQLEIIEEIISGGNVLAVLPTGAGKS
                     ICYQIPALISENYSIVVSPLIALMKDQVDSLNRSSVIAAFINSTQSFNETEKVLQDIH
                     FGKIKIVYVAPERLENPEFATRLKNLNPQYLFIDEAHCISEWGHNFRPSYTKLKDFIE
                     FSGIKKISAFTATATPEVVKDIVKQLGLKNAKVIIKGFERENLFVNVEIIKKKKERCL
                     ELVHQFKSPAIIYTSSRKKAEELAEYLKLNKIRCEFYHAGLDPIIRKKIQEDFIEDRL
                     PLIIATNAFGMGIDKKDIRLVIHFNATGTIENFYQEIGRAGRDGKNSHTFLLYDESDV
                     YIHEYFISNSYPTKEIIKNIYNAVCDSAQVAIGMISEKQIPINLSYIKLHTKQDISGA
                     ILNSSLKYLENAGYISINSVYKPLNKIKILFNETRLKAFIKGTSNELMRDILLHFIRN
                     YGKEIFSRLTAVDLISLQSETGLTKQETINT"
     gene            1639..>1899
                     /locus_tag="VF870_10420"
     CDS             1639..>1899
                     /locus_tag="VF870_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252648.1"
                     /translation="MKTILALLFLSIFTYAQVQYTDMLKPQSIYTEAPTEVKSRKAFM
                     REWWFYEQRSFPNDFIPVDAYKNALDQRELLRQSNEQLALTDN"
BASE COUNT          597 a          318 c          293 g          691 t
ORIGIN      
        1 atgtattaat agtttcttgt tttgttaatc ccgtttcaga ttgtaagctt attaaatcca
       61 cagcggttaa tcttgagaat atttctttcc cataatttct tataaagtgt aaaagtatgt
      121 ctctcatcaa ttcgtttgat gtgcctttta taaaagcttt taatctggtt tcattaaaaa
      181 gtattttaat tttattaagg ggcttataaa ctgagttaat gctgatgtaa cctgcattct
      241 ctaaatattt gagtgaagaa tttaaaattg ctcctgatat atcctgtttt gtatgcagtt
      301 taatataact taggtttata ggtatttgtt tttcggatat cataccaata gcaacttggg
      361 cggaatcgca cacagcgtta taaatatttt taataatctc tttagtagga tatgaattag
      421 aaataaaata ttcgtgtata taaacatcgc tctcgtcgta taaaagaaaa gtatgagaat
      481 tctttccatc tctgcctgca cgcccgattt cctgataaaa attttcaatt gtaccggttg
      541 cattaaaatg aatgactaac cggatatcct ttttatcgat tcccattcca aaggcattcg
      601 ttgcaatgat cagaggtaat ctgtcctcaa taaaatcttc ctggattttc ttccttatta
      661 tgggatcaag ccctgcatga taaaactcgc atctgatctt attaagtttt aaatactcag
      721 ctaactcttc agctttttta cgtgaggatg tataaataat agccggagat ttgaattgat
      781 gcacaagttc taagcatcgt tcttttttct tttttataat ctcaacattc acaaaaagat
      841 tttcacgctc aaatccttta ataattactt tagcgttttt taatccaagc tgtttaacta
      901 tatctttaac aacttctggt gtcgcggtgg ctgtaaaagc cgaaatcttt tttattcctg
      961 aaaactctat aaaatccttc agcttagtat aactggggcg aaaattgtgt ccccattcac
     1021 taatacaatg tgcttcatcg ataaataaat attggggatt taggtttttt aatcttgtag
     1081 caaattccgg gttttcaagt cgttctggag caacataaac aatcttaatt tttccgaagt
     1141 ggatatcctg taaaactttt tcagtttcat taaaactttg tgtgctatta ataaaagctg
     1201 caatcactga tgatcgattt aatgaatcga cctgatcttt cattaaagca attaatggag
     1261 aaacaacgat tgagtaattc tcacttatta aggctggaat ttggtagcaa attgattttc
     1321 ctgcacctgt aggtaagacg gcaagaacat ttcctccgct gattatctct tcgatgattt
     1381 caagttgtcc taaccgataa ttatcgtaat taaaatattt ttttagagcc ttctttaaga
     1441 tcataaaaaa tgttagtttt gccttagtta atgccaattt tgacggtgag tatttctagc
     1501 caaccacgca ataattactc tatacctgtc ttaaaaatac taaaaactta atctattttc
     1561 ttttttaatt ggtctgattt gtgaagcaga tatgtcgctg aaaaatcaat aattggagat
     1621 aatgaaagaa tgaataaaat gaaaaccatc ctagccttgt tattcctgtc aatatttaca
     1681 tatgcacaag ttcagtatac tgatatgctt aaaccccaaa gtatttatac tgaagcaccg
     1741 actgaagtaa aatctcgtaa ggcatttatg cgtgaatggt ggttttatga acagagatct
     1801 tttccaaatg attttattcc tgttgatgct tataaaaatg cacttgatca aagagaactt
     1861 ctcagacagt ctaacgaaca acttgcatta actgataac
//
LOCUS       DASWBY010000575         3385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_738427, whole genome shotgun sequence.
ACCESSION   DASWBY010000575 DASWBY010000000
VERSION     DASWBY010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738427"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3385)
                     /locus_tag="VF870_10425"
     CDS             complement(<1..>3385)
                     /locus_tag="VF870_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9252649.1"
                     /translation="KLNLARRNLRKSLAPENESYKEIVKIINDEVFADYSIEIFAPNG
                     KLIAWNQIIAIGQDELFPLSFPLGETYFLSKDLLNYLSVIDTTHLESDIFYIAISTPI
                     EEKFKINNQYSKNISFKNELFSKYQLDLNVEYSPYAEKSKDGRKYSFELLNTKNDKIG
                     MVSFTKPMLSSEIINIKDTASKIQSLLVVLAIFFVGLGLNSDFRKLDSLLFKFFLLVI
                     YLSALRAIIFIVGFPSNFVNGPLSDSSYFSSAFGWGIVKSPIEFLTTILFVVLVAVQI
                     FRYTRKFLFSSQNIKNKYFSYLIVLSLSVIIFLIIRGLSASIRSIIFDSTIRYFKEPN
                     ILPDLPSLVMNFNMLLLSFASVLSIVGLMNLIFKFSRIDIQNLKRSKVYLLFVVVGFI
                     LGLSFYISNDPLITPIMLLVFVLFLFFLFYQITFKNKISAYSYLFIILIGSVISISLL
                     NFFNTKLERESLKTTAFEINRADSNLLSFMLNETLTAIKGRDDIVRIFGDRYANFDAI
                     AFKIWANSPMQRESLNSGIRFYDKYKNVLGEYIIGLNPDKKVFDLLSKYDDINILEIE
                     SKEENTGKYFAGVIKIEERGIIKGYVSAFAKFDIKSIGAVNFPDFIESNLSILNRVID
                     VKQLKIFQFSGSNLQQVYGDIYPSRDQIKQILQTPFDTLYNDAWLKIRFDSETYETYL
                     LKNVNDGNEIITTVSVEEKAFSWNLFNFFKIFIIHSLFIVIAFLIILISRFGKFNLSF
                     KSKLLFAFLVISIIPVIVLALYNTKIVSERAKEGIFNELSQRANYIEKHVTSQIEKNK
                     NRDVKAASENAARELGISFALYESTDQIYNSKDIYNRIGFFDRKLNPQAYYHLNYLRY
                     QEYLASEKLNNYKFDSYYRVISLKNKDYILSVNDAFNKINILFSTTEINVVIFGIYSF
                     AVIIIIIISTLFANQISQPIQRLTEATDAVSKGDLNVKIDHREKGELKDLLDGFNQMT
                     SELKKNQIELAEMEREAAWKEMAKQVAHEIKNPLTPMKLALQQLIISYKDKSKDFDKL
                     FEKVSHTVLNQIDNLNQIASEFSRFAKMPSLKIETLDMLSVLNDTLNMFMHEKTSIKI
                     ITSLDNAIIDADLSQLRRMFVNLIRNSMQADASSIAIDVF"
BASE COUNT         1185 a          536 c          485 g         1179 t
ORIGIN      
        1 aaaaacatcg attgcaatac ttgacgcatc tgcctgcata gaatttctga ttaaattaac
       61 aaacattctt cttaactgac tgagatcagc atctataatt gcattatcca acgaggttat
      121 aatttttatt gatgttttct cgtgcataaa catatttaaa gtatcattta agactgagag
      181 catatccagg gtttcaattt taagactggg catttttgca aatcttgaaa actcagatgc
      241 aatttgatta agattatcta tctgatttaa aactgtgtgt gatacttttt caaaaagttt
      301 atcaaagtct ttgctcttat ctttgtatga aattatgagc tgctgtaaag ctaatttcat
      361 tggagtaagt ggatttttaa tttcgtgcgc aacctgcttt gccatttcct tccaggcagc
      421 ttcccgttcc atttccgcaa gttctatctg attctttttc aattcggaag tcatctggtt
      481 aaaaccatcc agaagatctt taagctcacc tttttctctg tgatctattt ttacattaag
      541 atcaccttta cttacagcat ctgtagcttc ggtcaatctt tgtattggtt gtgatatctg
      601 attcgcaaag agtgtactga ttataattat aattattacg gcaaaggaat atattccaaa
      661 tattacaaca tttatttcag tggttgaaaa aagaatattt attttgttga aggcatcatt
      721 aactgataat atataatctt tattttttaa actgataaca cggtaataag aatcaaattt
      781 ataattattc aatttttcag atgctaagta ctcctgataa cgtagatagt ttaaatgata
      841 ataagcttgc ggatttaatt ttctatcaaa gaatccaata cgattataaa tatctttaga
      901 attataaatc tgatctgttg attcatatag tgcaaatgat attcctaatt ctcttgcagc
      961 attttcagat gctgctttga catcacgatt tttatttttt tctatctgac tagtaacatg
     1021 tttttcaata taatttgctc tttgacttaa ctcattaaaa atcccttcct ttgctctttc
     1081 gctaacaatc ttagtattat acagcgctaa aacaataacc ggaataattg agataaccag
     1141 aaacgcaaac agcagtttag atttaaaact taaattaaat ttcccaaaac ggcttattag
     1201 aatgataaga aaagcaatta cgataaataa tgaatgaata ataaatattt tgaaaaagtt
     1261 aaataggttc catgaaaatg ctttttcctc aaccgagaca gtagttataa tttcattccc
     1321 gtcattaaca ttttttaaca ggtaagtttc atatgtttca gaatcaaatc ttatttttaa
     1381 ccaggcgtca ttataaagag tatcaaaggg tgtctgtaat atttgcttta tctgatctcg
     1441 cgacggataa atgtctccat aaacctgctg caaatttgat ccgctaaact gaaaaatttt
     1501 gagctgcttt acatcaatta cacgattaag aatagataga tttgactcaa taaaatcagg
     1561 aaaattgaca gctccaatag attttatatc aaactttgca aatgccgaaa catacccctt
     1621 aatgattcct ctttcttcaa tcttaataac accggcaaaa tattttcctg tattttcttc
     1681 tttactttca atttctaaaa tatttatatc gtcatatttg cttaataaat caaatacttt
     1741 tttatcaggg ttcagtccaa taatatactc gcctaaaaca tttttatatt tatcataaaa
     1801 tctaataccc gaattaagcg actcgcgctg cattgggctg tttgcccaaa ttttaaatgc
     1861 aattgcatca aaatttgcgt agcgatctcc aaatattctt acaatatcat ctcttccttt
     1921 tattgcagtt aaagtttcat taagcataaa agaaagaaga tttgagtccg ctctgtttat
     1981 ttcaaaagca gtggttttaa gtgactctct ttcaagtttt gtgttaaaaa agtttaagag
     2041 tgaaattgaa attacagatc cgatcagaat tataaataaa tagctgtatg ctgagatttt
     2101 atttttgaat gtaatttgat aaaaaagaaa aaacagaaat aatacaaaca ctaaaagcat
     2161 aattggagtt attaaaggat cattgctgat ataaaatgat aaacccaaaa taaatccaac
     2221 aacaacaaac aatagataga ctttacttct cttcagattt tgaatatcaa ttcttgaaaa
     2281 cttaaaaatc aggttcatca ggccaactat tgagagtact gaagcaaatg aaagaagaag
     2341 catattaaaa ttcattacca gagaaggtaa atcaggcaga atattaggtt ctttaaagta
     2401 ccgtattgtc gaatcaaaaa ttatacttct gattgacgca cttagtcctc ttataattaa
     2461 aaaaataatt accgataatg acagtactat caagtaactg aaatatttat ttttaatatt
     2521 ttgtgaggaa aacaagaatt ttctggtata tctaaaaatt tgaactgcca ctaatacaac
     2581 aaaaaggatc gtagttaaaa actcaattgg tgattttacg attccccagc caaatgcaga
     2641 tgaaaaatat gatgagtctg acagcggacc atttacaaaa ttggatggaa atccaacgat
     2701 aaaaattatt gcacgcagag cggataaata aatcacaaga agaaagaact tgaataacag
     2761 agaatcaagt ttccgaaagt cgctgttcaa ccctaatcca acgaaaaata ttgcaagcac
     2821 tacaagtaaa gactggattt tagaggccgt atctttgatg tttattattt cactgcttaa
     2881 cattggtttg gtaaatgaaa ccatacctat tttatcgttt ttagtattta ataattcaaa
     2941 agaatatttt ctgccatctt tggatttttc agcgtaaggt gaatactcca catttaaatc
     3001 aagctgatac tttgaaaaaa gttcattttt gaaactgata ttttttgaat actgattatt
     3061 gattttaaat ttttcctcaa tcggagttga aatagcaatg taaaatatat cggattcaag
     3121 atgagttgta tctataactg ataaataatt aagtaaatct ttagaaagga aatacgtttc
     3181 tcccaaagga aaacttagcg gaaataactc atcctgaccg attgcaatta tctgattcca
     3241 ggcaattagt ttaccgttcg gggcaaatat ttctattgag tagtcagcga aaacctcatc
     3301 gtttataatt ttaactatct ctttatacga ttcattttcg ggtgcaagtg attttctaag
     3361 attacggcgg gccagattta atttt
//
LOCUS       DASWBY010000576         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_249474, whole genome shotgun sequence.
ACCESSION   DASWBY010000576 DASWBY010000000
VERSION     DASWBY010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249474"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF870_10430"
     CDS             complement(<1..458)
                     /locus_tag="VF870_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX9252650.1"
                     /translation="MYTNICPNCGEKSDREERDCPNCGFAKAEIVNRNSITKSINDSL
                     VNKLSFFDYPQLYVTVIFRLVFILITIFLIPDFISNSEFFWMVVGIFSVYGLIKEIAN
                     VYPWFILVYKISKIILFAYFGAGLGNLISHTGRLVYYRFSYYFNLEFLYN"
     gene            complement(465..>2146)
                     /locus_tag="VF870_10435"
     CDS             complement(465..>2146)
                     /locus_tag="VF870_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9252651.1"
                     /translation="PSITSIQDMLLRSAKVYGDKLALEDLIDNPISSVTFKQLHDYMI
                     RFGKSLFEIGLKERDHVAVISENRVQWSLTYFTSMCFNMVIVPIDKNLNHNEILNIIH
                     ESEAKAIVFSDGFEALMKEKRDSLLNLKYYINMDSKEHKDKIFSMAKMIEAQSASIER
                     LPFVNPEDMAEIIFTSGSLGRAKGVMLTQKNIASNLMAMTSMIQIVPEDRFLSVLPIH
                     HKYECTCGLLCPLYAGASAHYARSLKTVVDDLQKVKATLLLGVPLLYDKMFKKIYKGI
                     QEDKLKSIIVPPLISITNVFESIGWKSSKKLIFKELHHKFGGHIRLFIAGGAAPDPKV
                     AKGLRELGFNFVQGYGLTETSPIVALNRLYSFKDNAAGLPLPGLEIKINNPNEIGAGE
                     IYIKGDSVMLGYYKNQKLTDEAFDNGWFKTGDIGFFDEDGFLHINGRQKNVIISKSGE
                     NVFPEEIEDILNRNPFVQECMVYGEEDEKHTEIIAVQIVTDAEAFIEYSEKNKVNITP
                     ELVNDIISEAVKEVNKELPVYKQIRKFHIRESEFEKTTTQKIKRFLVQNAN"
BASE COUNT          680 a          401 c          317 g          748 t
ORIGIN      
        1 tgattataca aaaattcaag attaaagtaa tagctgaatc tatagtacac taatctccct
       61 gtgtgtgaaa ttagattgcc aagaccagcc ccaaagtatg cgaacaaaat tattttacta
      121 atcttgtaaa caagaataaa ccagggataa acatttgcta tttctttaat caatccataa
      181 acagaaaaaa taccaacaac catccagaaa aattcactgt tgcttataaa atcagggata
      241 aggaatatgg taataaggat aaagactaag cggaaaatta ctgtaacata aagctgaggg
      301 taatcaaaaa aacttaattt attaacaagt gaatcattaa tgctttttgt aatactgttt
      361 ctgttaacta tttcagcttt agcaaatccg caattaggac aatctctttc ttccctatcg
      421 gacttttctc cgcaatttgg acatatgttt gtgtacatta ttttttaatt agcattttga
      481 accaaaaatc ttttaatttt ttgtgttgtg gttttctcaa attcgctttc tctgatatga
      541 aattttctta tttgtttata caccggaagt tctttgttaa cctccttaac cgcctcagaa
      601 ataatatcat taaccagctc cggcgtaata ttaactttat tcttttcaga atattcaata
      661 aacgcttcag catccgttac aatttgtact gcaattattt cggtgtgttt ttcatcttct
      721 tcaccgtaaa ccatgcattc ctgcacaaag ggatttctgt ttaatatatc ttctatttct
      781 tcaggaaaaa cattttcacc gcttttagaa attataacat ttttttgtct gccgttgata
      841 tgtaaaaatc catcttcatc aaaaaagccg atatccccgg ttttgaacca gccattatca
      901 aaagcttcat cagtaagttt ttgattttta taataaccga gcatcacgct atctcctttt
      961 atgtatatct ccccagctcc gatttcattc gggttattaa tttttatttc aagtccgggc
     1021 aatggtaatc cggcggcatt atctttaaag ctgtataatc tgtttaacgc aacaataggt
     1081 gatgtttcag ttaagccgta accctgaaca aaattaaatc caagttcgcg taaacctttt
     1141 gcaactttgg gatcaggtgc ggcaccgccc gcaataaata atctaatatg tccgccaaat
     1201 ttatggtgca gctctttaaa gattaatttt ttagaactct tccagccgat tgattcaaat
     1261 acgtttgtta ttgatataag cggcggaaca attatcgatt ttaatttatc ctcctgaatt
     1321 cctttataaa ttttcttaaa cattttatca tacaaaagcg gaactccaag taataatgtt
     1381 gctttaacct tctgtaaatc gtcgacaact gtttttaatg atcgggcata atgtgccgaa
     1441 gcacccgcgt ataatggaca cagcaaacca caagtgcatt catatttatg gtgaattgga
     1501 aggactgaaa gaaatctgtc ttcaggtaca atctggatca tacttgtcat tgccataaga
     1561 ttggatgcaa tatttttctg cgtaagcata acaccctttg ctctgccgag cgatcctgat
     1621 gtaaatataa tttcagccat atcttcagga ttgacaaatg gtaatctttc aatcgatgca
     1681 gattgtgctt caatcatttt cgccatcgaa aatattttat ctttgtgctc tttcgaatcc
     1741 atgtttatat aatatttaag attcaaaagc gaatcccttt tctctttcat caaagcttca
     1801 aatccgtctg aaaaaacaat ggctttagcc tcagattcat gtatgatgtt taatatttca
     1861 ttatggttaa gatttttatc aataggaaca ataaccatat taaaacacat tgatgtaaag
     1921 tatgtaagcg accattgtac acggttttca ctgattacag caacatggtc tctttccttt
     1981 aatccaattt caaaaagtga tttaccaaat cgtatcatat aatcgtgcag ttgtttaaat
     2041 gtaacacttg aaattggatt gtctataaga tcttcaagcg ccagtttatc accataaact
     2101 tttgctgatc tcaaaagcat atcttgaatt gaggttatgc ttggaa
//
LOCUS       DASWBY010000577         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_98029, whole genome shotgun sequence.
ACCESSION   DASWBY010000577 DASWBY010000000
VERSION     DASWBY010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98029"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VF870_10440"
     CDS             complement(<1..874)
                     /locus_tag="VF870_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9252652.1"
                     /translation="MIESSKNSKILVCDDDETLCYLLKEQLLEEGFTVDAVYDGKYAI
                     EAVKTKNYDILLLDLNMREVQGEDVLKFIKDSGYNIQVIILSAQSDMKKAIQCIKNGA
                     YDYINKPYEFEELVLTVNRAIEHRNLLVTTVLLSKEISKKHSEKIIGDSKSLHRVLNL
                     ANKAAQSDSNVLVEGETGTGKELFAEYIHKQSARKDKPFVVINCASLPDQLIESELFG
                     HEKGAFTDAKSTKQGLVEIAHGGTLFLDEIGELSLTLQPKLLRFLENGEYRRIGGVTN
                     LNSNVRVIGATNRNL"
     gene            947..1018
                     /locus_tag="VF870_10445"
     tRNA            947..1018
                     /locus_tag="VF870_10445"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:979..981,aa:Lys,seq:ttt)
     gene            1235..1498
                     /locus_tag="VF870_10450"
     CDS             1235..1498
                     /locus_tag="VF870_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252653.1"
                     /translation="MLTLWFTKDFFGEKFFWIISALAIYLFVFHPTYSAYKNFIDENK
                     NVITNSLCSTCKHFNESAVLCTKYDKHPTENFIPCEGTAWEPK"
     gene            1626..>2003
                     /locus_tag="VF870_10455"
     CDS             1626..>2003
                     /locus_tag="VF870_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252654.1"
                     /translation="MKKLYSLPFLFLTALLFTFNSISAQGFLHRQDKKIVKGNGQEIF
                     LKGIGLGGWLLQEGYMLHTSDFANAQWQIRAKILDLVGETNTELFYQTYRNNYVRKAD
                     IDSIKSWGFNSIRLPFHYNLFATN"
BASE COUNT          633 a          353 c          312 g          705 t
ORIGIN      
        1 ataaatttct attggtcgcg cctatcactc taacatttga atttaaattt gtaacaccac
       61 cgattcttct atactcgcca ttttctaaaa atcttaaaag cttgggttgc aaggttaaac
      121 ttagctcacc aatttcatct aagaataagg tgccaccatg agcaatttct accaatccct
      181 gctttgtaga tttagcatca gtaaatgccc ctttttcatg accaaacaat tcactctcaa
      241 taagctgatc gggcagagaa gcacagttaa taacaacaaa aggtttatct tttcttgcag
      301 attgtttgtg tatgtattca gcaaatagtt ctttgcccgt gccggtttca ccttcaacaa
      361 gaacattaga atcagattgt gcggctttat ttgcaagatt taatacacga tgtaatgatt
      421 tactatcccc aataattttt tcagaatgtt ttttactgat ctcttttgag agcagaacgg
      481 ttgttaccaa taaatttcta tgttcgattg ctctgttaac agttaagaca agttcttcaa
      541 attcatatgg tttgtttata taatcgtagg caccgttttt aatacattga atagccttct
      601 tcatatcaga ttgtgcggaa aggataataa cctgaatgtt ataacctgaa tcctttatga
      661 attttaatac atcctcacct tgtacttccc tcatattaag atcaagaaga agtatatcat
      721 aattttttgt tttaactgcc tctatggcgt atttgccgtc ataaacagcg tcaacagtaa
      781 agccctcttc taatagttgc tcttttaaca agtaacaaag ggtttcgtcg tcatcacaaa
      841 caagaatttt ggagtttttt gaactttcta tcatttccca ccggtaaaaa aatctgttag
      901 tttggattaa aaatgcacat taattatatt tgttgttcaa gttttcgggc gcgtagctca
      961 ggggtagagc atctgccttt taagcagagg gtcggtggtt cgagcccacc cgcgctcacg
     1021 acaagcaaag ccacttttgt ggctttgttt attttaaaca atttttaaga accaatgttt
     1081 aataataaca catattacca aaaacagcaa gtttaagcgc ttttaactat tttaatattc
     1141 gtgctttatc gattaaataa ttttatgctt acttcattct aggattgatt tgggtgagat
     1201 agtttataaa actttaataa ggattatcct ccttttgcta accctttggt ttactaaaga
     1261 tttttttggt gaaaaatttt tctggattat aagcgctttg gctatctact tattcgtttt
     1321 tcatccaact tattcagctt ataaaaattt tattgatgaa aacaaaaatg taattacaaa
     1381 cagcttgtgc tcaacctgta aacactttaa tgaatctgct gttttgtgca caaaatacga
     1441 taaacatccc acggaaaatt tcattccttg tgaaggaacc gcttgggaac ccaaataaaa
     1501 aatgatattc taaatttatc ggttgctatt aattcgtcct gataaaaaca atttaatgtt
     1561 aatttactac tatattttgc cttctatttg gtgttgaaca ataacatatt ataatgagat
     1621 tctttatgaa aaaattatat tcacttccct ttctctttct tactgcatta ctttttacat
     1681 ttaacagtat ttcagcacaa gggtttttac accgacagga taaaaaaatt gttaaaggta
     1741 acggacaaga aatttttcta aagggaattg ggcttggtgg atggctgctg caggaagggt
     1801 acatgcttca tacctcggac tttgcaaatg cacaatggca aatacgagca aaaatattag
     1861 accttgtggg cgaaacaaat acagaactgt tttatcaaac atatcgaaat aattatgtaa
     1921 gaaaagcaga tatagattct ataaaaagtt ggggatttaa ttctatcaga cttccatttc
     1981 attataatct ttttgcaaca aac
//
LOCUS       DASWBY010000578         3706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_619930, whole genome shotgun sequence.
ACCESSION   DASWBY010000578 DASWBY010000000
VERSION     DASWBY010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619930"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..493)
                     /locus_tag="VF870_10460"
     CDS             complement(11..493)
                     /locus_tag="VF870_10460"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX9252655.1"
                     /translation="MIISIIVAIARNGVIGKANGEMSWHVKEEFKHFKDTTLGFPIIM
                     GRKTFETLGKPLKGRLNIVVSKNKSYKTFYDEVVIKSSIENAIEYCKPLTPGKIFIIG
                     GGEIYRQAILFVDEMMITFMKFEAEGEVKFPNINNDDWTIEKIKDHELFEIFRYVRKS
                     "
     gene            complement(490..1377)
                     /gene="thyA"
                     /locus_tag="VF870_10465"
     CDS             complement(490..1377)
                     /gene="thyA"
                     /locus_tag="VF870_10465"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX9252656.1"
                     /translation="MKEYLDLVKLVIDEGTHKKSRTGIDTISYFGAFYKVDLQKGFPL
                     LTTKKVEWKSLLKEVLWYLSGENHIRNLRQHTKIWDAWADENGRLQTAYGRFWRRFPV
                     PEANAYLEGEVFVDENNPFVHKEENGSLTFDQVGWVINEIKNNPNSRRLVISAWHPAN
                     AIVSKLPPCHYTFAFNVNAGKLNCHLTQRSGDIALGIPFNLAAYSMLTQIIAQETNLH
                     LGSFAHTIVDAHIYHAAKGSELERFDHLEGLKLQLLREPLALPKLKIAKKPIDELTFE
                     DFELIDYKYHDKIKFEVAV"
     gene            complement(1456..2583)
                     /locus_tag="VF870_10470"
     CDS             complement(1456..2583)
                     /locus_tag="VF870_10470"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX9252657.1"
                     /translation="MRILIAPNSFKECADSVEISDLINKHLSKNNSIKTVLKPLSDGG
                     DGFLSVIKKLFDTVEYEIPIKDEFNQAKCYKILINKKNETAYLESASVIGLKHIEEKN
                     RRPMQLNTSVIGKIIHQLSVEVNQSKLKIKNLVIGIGGTATIDFGIGACSQLDLSLFD
                     NNGILLSPDPQNFNDVYSIKFVKPDLPFTIKCIVDVDTALIGEPGAIEIYGKQKGASD
                     EELKKIKSGIKNILNLISADERFEIPNNLNGAGGGLAAGLNIFLNAELIKAEEFIKNF
                     ILKDLKLEEIDSVITGEGSFDFQSFEGKGAGIILQLFEKKNIPIFLINGSTNLPTDIN
                     LSENVLVINLIDFFGSKKESIINYRTGIEKAAKIVLYHLNK"
     gene            complement(2580..3599)
                     /locus_tag="VF870_10475"
     CDS             complement(2580..3599)
                     /locus_tag="VF870_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9252658.1"
                     /translation="MKQLRFLIARIDRIGDVVLSTPLPREIKKKYPDSYVAVLVRHYT
                     KDIYLNNPNVDEIITIDNADKTEKSFWNLVTEIKQYKFTHAFMLLPNEKINYTLFMSG
                     IKYRVGVGHKLYQFLTFTKYVDRKKYIPLRHEADYCLDMIRKIGVEVESINPEIYLTE
                     NEKDKIRDFRKYNRQGNKILIGINISSGNSAPNLNGGEYKKLIILLKQNHDYKIAVMD
                     NNVLPEIDNIENVLYPNMGNSLRDSIINLASLDLLISNSTGPMHICAALSVPTVSLFC
                     PLTACSPKLWGPLGNKSEIIMPAQIYCDTKCPIDPKKCDYSKEGGITAEEVAFKVDKF
                     LNDLR"
BASE COUNT         1156 a          683 c          545 g         1322 t
ORIGIN      
        1 cgtgaggcca ttaactcttt cttacatatc taaatatttc aaacaattcg tgatctttta
       61 ttttctcaat ggtccaatca tcattattta tgttgggaaa tttaacttcc ccctcagctt
      121 caaatttcat aaatgtaatc atcatttcat caacaaataa aattgcctgt ctgtaaattt
      181 caccgccgcc aatgataaag atttttcccg gcgtaagagg tttacaatat tcaatggcat
      241 tctcaattga ggatttgata acaacttcat cataaaaagt tttatatgat ttatttttac
      301 ttacaacaat attcaatctg ccttttagcg gcttgccaag agtttcaaaa gtttttcttc
      361 ccataattat aggaaaacct aaagtagtat ctttaaaatg tttgaactct tcttttacgt
      421 gccaggacat ttctccgtta gcttttccta taacaccatt tcttgcgatt gcaacaataa
      481 tgcttatgat caaaccgcaa cctcaaattt tattttatca tgatatttat aatcaatcaa
      541 ttcaaaatct tcaaatgtta attcatcaat tggctttttt gcgatcttaa gttttggcaa
      601 agctaatggt tcacgcagca actgcagctt taatccttca agatgatcaa acctttctaa
      661 ctcagagccc tttgcagcat gataaatatg agcatccacg attgtatgag caaacgatcc
      721 aagatgcaaa tttgtttcct gagcaattat ctgggtaagc atagagtatg cggcaagatt
      781 aaatggaatt ccaagtgcaa tatcaccgga acgctgagtt aaatgacaat ttaattttcc
      841 ggcattgacg ttaaaagcaa aagtgtaatg acagggcggc aatttgctga ctattgcatt
      901 tgccggatgc catgcagaaa taactaatct acgagagttg ggattatttt ttatttcatt
      961 tatcacccaa ccaacctgat caaaagtcaa tgaaccattt tcttctttat gaacaaatgg
     1021 attattttca tctacaaaaa cttccccttc taaataagca tttgcttccg gaacggggaa
     1081 tcttctccaa aatcttccat aagcagtttg taatctccca ttttcatctg cccaggcatc
     1141 ccagattttt gtgtgctgtc ttaaatttct aatatgattt tcacctgaaa gataccagag
     1201 cacttccttc aataaggact tccattccac cttttttgtt gttaaaagcg gaaatccctt
     1261 ctgtaaatca actttataaa atgcaccaaa gtaagaaatt gtgtcaattc ctgttctcga
     1321 ttttttatgc gttccttcat caattactaa tttaaccaga tcgagatatt ctttcattca
     1381 aataatcctg tgatttttta gaatagttaa taattttcgt gtgaatttac taaaaaaaga
     1441 tgaagtggta aaagcctatt tattaaggtg atacaggaca atttttgctg ctttttcaat
     1501 acctgtacga taattaataa ttgactcttt ttttgagcca aagaaatcta tcaaattaat
     1561 aacgagcacg ttttcagaca ggtttatatc ggttgggaga ttagtagaac cattaattag
     1621 aaatattggg atgttttttt tctcaaataa ttggagaatt attcccgcac cttttccttc
     1681 aaaagattga aaatcaaaac ttccctcacc agtaataaca gaatcaattt cttcaagttt
     1741 taaatctttt agaataaagt tcttaataaa ttcttctgct ttaatcagtt ctgcgttcag
     1801 gaaaatattt aatccggcgg ctaaccctcc accagcgcca tttaaattat ttggaatttc
     1861 aaatctttca tcagctgaga tgagatttaa aatatttttt atacctgatt tgattttctt
     1921 aagttcctca tctgaagcgc ctttttgctt gccataaatc tcgattgctc caggctcacc
     1981 aattagagca gtatcaacat ccactataca ttttatagta aaaggtaaat caggtttaac
     2041 gaatttaatg gagtaaacat cattaaagtt ttgtggatcc ggtgatagta gaataccatt
     2101 attatcaaat aaacttaaat caagctgcga acaagcacca attccaaaat caatcgttgc
     2161 ggttccacca attccaatta ccaagttttt tatttttaat ttcgattgat tcacttcaac
     2221 tgaaagctga tgaataatct tgccaattac agaagtattt aactgcattg gtcttcgatt
     2281 tttttcttca atgtgcttaa gcccaatcac tgatgctgat tcgagataag ctgtttcatt
     2341 ctttttattg attaatattt tgtaacattt tgcctgatta aattcgtctt taattggaat
     2401 ttcatactct actgtatcaa aaagtttttt aattacagat aaaaatccat cgccgccatc
     2461 tgaaagaggc ttaagtacag ttttaatcga attatttttg gaaagatgtt tatttattag
     2521 atctgatatt tcaacggagt ctgcacattc tttaaaacta tttggagcaa taagaattct
     2581 catcgcaggt catttaaaaa tttatcgact ttaaaagcca cttcctcggc tgtgataccg
     2641 ccttcttttg agtagtcaca ttttttagga tcaatcggac atttagtatc acaataaatc
     2701 tgagcaggca ttataatttc agatttatta ccaagtggtc cccaaagttt tggtgaacag
     2761 gcagtaagcg gacagaaaag agaaactgtt ggaacactta atgccgcaca aatatgcatt
     2821 ggtccagtgc tatttgagat gagcaaatct aatgacgcaa gatttataat tgaatcgcgt
     2881 agcgaatttc ccatatttgg ataaagaaca ttttcaatgt tatctatttc cggcaataca
     2941 ttattatcca ttacggctat tttgtaatcg tggttttgct tcaacaatat tattagtttt
     3001 ttgtactcgc ctccatttag gtttggtgct gagtttccag aagaaatgtt tattccaata
     3061 agtattttat tgccttgccg attatacttt ctgaaatccc gaattttatc tttttcattt
     3121 tcggtcaaat atatttccgg attaatactt tctacctcta cgccaatttt cctgatcata
     3181 tccaaacaat aatctgcttc gtggcgcagc ggaatatatt ttttccgatc tacatatttc
     3241 gtgaaagtta aaaactggta aagtttatgc ccaacaccca cgcggtattt aattccagac
     3301 ataaaaagag tataattaat tttttcattt ggcaggagca tgaatgcgtg cgtaaactta
     3361 tactgcttta tttctgtcac taaattccaa aaagattttt cagtcttgtc ggcattatca
     3421 atcgtaatta tttcatctac gttaggatta tttagataaa tgtcttttgt gtaatgcctg
     3481 actaaaacag caacataact atccggatat ttttttttga tttcacgagg cagaggagtc
     3541 gaaagcacaa catcgcctat tctgtcaatt ctggcaataa gaaatctcaa ctgcttcata
     3601 agattatgat tttttattct tctttatttc cataatctta agctgtacct gaagttttgt
     3661 aaccatatgc atataagaaa tgataactcc ataaattccg tctaaa
//
LOCUS       DASWBY010000579         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_27182, whole genome shotgun sequence.
ACCESSION   DASWBY010000579 DASWBY010000000
VERSION     DASWBY010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27182"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /locus_tag="VF870_10480"
     CDS             complement(<1..1774)
                     /locus_tag="VF870_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252659.1"
                     /translation="MKNVLFVFLVSAAAGFITFSFFTNHSISQKINFIISTGDERGPD
                     LRPNEMAYIKKTYPYYNADSDVYIRALEQAQQLKKETIASRLNKGTRAVQWEFAGPTN
                     IGGRVVDIEFDPNNPSIIYAGFSTGGVFKSFDAGNTWNPIFDELAVLTVGDIAIDPNN
                     SNVIYVGTGEANGGHNNFPGGGVFKSTDAGLTWNFLGLEGTTSIGRIVINPQNSDILY
                     LASVGSYFAQNPERGVYKSTDAGLTWNHSLFVSDTTGAVDIIIDPNNPDRLMATTWER
                     VRRPNSSRLFGPTSGIYKTTDGGNNWIKITTAPLPNPNFTNVGRIGLAIHASDPNIVY
                     ALFTDGYNIICLYRSNDFGISWNNADMDNELSSGTAGFSWYFGQVRVHPTNPNIVYVL
                     DVAFMRSTDGGNNWPVIYGYGGPSQLHVDHHAMAFNPADPNIIINGNDGGINISTDGG
                     QTFSQPKNIPATQFYEIGLDFNNPQKLYGGTQDNGTNRTPDGGIDNWESIFGGDGFYV
                     LIDNNDPNIIYAESQNGGLGKSTNGGSSWVGATNGIISSEPTNWSTPVVMDPNSSNIL
                     YYGTHSLYRTTNGAASWTKISPQLT"
     gene            1977..>3094
                     /locus_tag="VF870_10485"
     CDS             1977..>3094
                     /locus_tag="VF870_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252660.1"
                     /translation="MKSRLVLLPIIILVLTFTKCSPGPKGQAPLFTNLGSHHFPVTTN
                     SELAQKYFNQGIILAYGFNHEEAFRSFKEVARLDSNCAMAYWGMAYVLGPNINLPMDS
                     GVIHTSYEAIQKAILMLDDESQREKDYVMALSKRYSNEKLDDRTPLDQAYSDAMRELA
                     KNYPEDLDAATMFAESIMDLHPWDYWLKDGTPQPWTPEILSTLEGVIEKETNHHGANH
                     LYIHAVEASKNPERGLASADRLRFLAPGAGHLVHMPSHIYIRVGKYHEGSLANMRAVK
                     SDEEYINQCNQQGFYPLSYYPHNYHFLWATATLEGDSKTAIDAALKTSQKPPDSLMDV
                     CGYQTLQHFAVIPLYAYVTFGKWDEIMQYKEPQDKRPYS"
BASE COUNT          954 a          618 c          554 g          968 t
ORIGIN      
        1 cagttaactg cggactaatc tttgtccagc ttgcggctcc atttgtagtt ctgtacagtg
       61 agtgtgttcc ataatataaa atgtttgaag agtttggatc cattacaacc ggagttgacc
      121 agtttgtcgg ctcgctgctg ataattccat tggttgcacc tacccagcta gagccgccgt
      181 ttgtcgattt tccaagtccg ccgttttgag attcagcata aattatattt ggatcgttat
      241 tatcgattaa aacataaaaa ccatctccgc caaaaatgct ttcccaatta tcaataccac
      301 catcgggtgt tctgttggtt ccgttatctt gcgtgccgcc gtaaagtttt tgcgggttgt
      361 taaaatctaa acctatctca taaaattgag ttgcagggat attctttggt tgtgaaaagg
      421 tttgtccgcc atctgtggaa atattaattc caccatcatt gccattaatt ataatgttag
      481 gatcagccgg attaaaagcc attgcgtggt gatcaacatg aagttgtgat ggaccgccat
      541 agccgtaaat aaccggccag ttatttccgc cgtctgttga tctcataaag gctacatcta
      601 aaacgtaaac aatatttgga tttgttggat gaactcttac ttgcccaaag taccagctga
      661 aacccgcagt accgctgctt aattcgttat ccatatctgc attattccag cttataccaa
      721 aatcatttga tctataaaga caaataatat tgtatccgtc tgtaaaaagt gcgtagacaa
      781 tattgggatc acttgcatga atcgctaaac caattcttcc aacgtttgta aagtttggat
      841 taggcaaagg agcagttgtt atcttaatcc aattatttcc cccatcagta gttttataaa
      901 ttccgcttgt tggcccaaac aaccgggatg aatttggtcg tcttactctc tcccatgttg
      961 ttgccattaa tctgtcagga ttatttggat caataataat atcaactgca ccggttgtat
     1021 ctgaaacgaa caaagaatgg ttccatgtta atccggcatc tgtggattta taaacacctc
     1081 tttcaggatt ttgtgcaaag taagatccaa cagaagcaag atacagtata tcagaatttt
     1141 gcggattaat tacaattctt ccgattgatg ttgttccttc aagcccaaga aaattccagg
     1201 tcaatcccgc atcagttgat ttaaaaacac ctcctccggg aaaattattg tgtccgccgt
     1261 ttgcttctcc tgttccaaca taaataacat tgctgttatt tggatcaatt gcaatatctc
     1321 caacagtaag cactgctagt tcatcaaaaa taggattcca tgtattaccc gcatcaaaag
     1381 atttgaaaac tccaccggta gaaaatcccg cataaattat acttggatta tttggatcaa
     1441 actcaatatc cacaactctg ccgccaatat tagttgggcc ggcaaactcc cattgcacag
     1501 cccgcgttcc tttgtttaat ctgcttgcaa tagtttcttt cttcagttgc tgtgcttgtt
     1561 caagggcacg aatgtataca tctgaatctg cattataata aggataagtt ttttttatgt
     1621 atgccatttc gtttggacga agatctggac cgcgctcatc accagtagat ataataaaat
     1681 ttattttttg cgagattgaa tgattggtaa aaaaagaaaa ggtgataaat cctgcggcag
     1741 cactaaccag aaaaacaaat aacacatttt tcatattcta tccaaacatt ataattgaaa
     1801 aaagattcaa acttatttta caaatgtttt ataaagattt ctacgaaatt ttgatgccaa
     1861 aactattgac atatcgcaat tattaatata ttttcaaacc ccaaacttag cattgtcctg
     1921 atgcttttta ggttttaatt aataaatccc ttaaataatt atcataggag gcaactatga
     1981 aatcaagatt agttttatta cccataatca ttctggttct aactttcact aaatgctcac
     2041 ctggcccaaa aggacaggcg ccattattta ctaaccttgg ttcacatcat tttccggtta
     2101 ctacaaattc agaacttgca caaaaatatt ttaatcaggg aattatactt gcatatgggt
     2161 ttaatcatga agaagctttt cgttccttta aagaagttgc aagattagat tcaaactgtg
     2221 ctatggcata ttggggaatg gcatatgttt taggtcctaa tataaacctg ccaatggatt
     2281 ctggtgttat acacacttct tatgaagcta tacagaaagc aattttaatg cttgatgatg
     2341 aaagccagcg cgagaaagat tatgtaatgg ctctttcaaa aagatattct aatgaaaaac
     2401 ttgatgatcg aacaccgctt gatcaggctt actctgatgc gatgagagaa ctcgcaaaaa
     2461 actatccaga agatcttgat gccgcaacaa tgtttgctga atctataatg gatcttcatc
     2521 catgggatta ctggttgaaa gatggaacac cacaaccctg gactccagaa atcctttcta
     2581 ctcttgaagg tgtaattgag aaagagacaa accatcacgg agccaatcat ctttatatac
     2641 acgcagttga agcttctaag aatccggagc gtggacttgc aagtgcggac aggttaagat
     2701 ttcttgcccc gggcgcagga catctggttc atatgccctc acacatttat ataagagttg
     2761 gtaaatatca tgaaggttct cttgcaaata tgcgtgccgt aaaatctgat gaagagtaca
     2821 tcaatcaatg caatcaacaa ggattttatc ctctttcgta ttatcctcat aattatcatt
     2881 ttttgtgggc aacagctacg cttgaaggcg acagcaaaac ggcaatagat gcggcactta
     2941 aaacttctca aaaaccacca gattcattaa tggatgtttg cggatatcag actctgcagc
     3001 attttgcggt tattccatta tatgcttatg taacattcgg taaatgggat gaaataatgc
     3061 agtataagga accgcaagat aaacgcccat actc
//
LOCUS       DASWBY010000580         3043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_477860, whole genome shotgun sequence.
ACCESSION   DASWBY010000580 DASWBY010000000
VERSION     DASWBY010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477860"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VF870_10490"
     CDS             complement(<1..576)
                     /locus_tag="VF870_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252661.1"
                     /translation="MKKLFSMIAALTLLLHFLTVAQSNSPSSANNVQVAFPFYDDVET
                     NATSSAYWDRDTLQWKIQITNAHSGTQVWAMLPFNGSYNYLTLASNIDLSSAPNPYLS
                     FWVRKADGGGGYVSIEASNDGGNTWSTIWQPYFSGTLYNRLQVSLGNFRQANARIRIG
                     CYGYSGGTYYIDDILIDNAPTPQPIVLSLPQN"
     gene            complement(1067..>3043)
                     /locus_tag="VF870_10495"
     CDS             complement(1067..>3043)
                     /locus_tag="VF870_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9252662.1"
                     /translation="NNITFNFSSLDFNSSEHIHFLYRLEGFDKDWIYSTKRRFAAYNN
                     LNPGTYYFRLKATNSDGIWGKTVKSIKVTINPPWWKTGWAYSVYILVIFLGLVGIRRF
                     ELNLIKLRHEIKIRDFESKKHRDIENVKSRFFANLSHEFRTPLMLIKGPLEQLKNGRV
                     KGDPQNYYDIIYRNTENLHTLIDQLLELTQLEAEAIPVKAREVNLITLLRGIFFSFES
                     IANEKNISIEFVSDDKSICAWVDRDKLEKIINNILSNAFKFTAEGGIISLAVKKIITG
                     NEEFARIKISDTGIGIPEDKLHKIFDRFYQVDDSSGRVYGGSGIGLALVKELIDLHKW
                     EINIESEIEKGTTFILTIPLSEYYLSDNQKIKDEIRSEDILEAVTDKSYNQDVITESN
                     NAVKDQNISDDKLTTNNPSILVVEDSSDVQEYIYSLFQNDYKIIQAMNAEEGIIKASE
                     QMPDLILSDVMMPGMGGLEFCKQIKTNFQTSHIPIILLTAKVSSENKIEGLETGADDY
                     VTKPFNFKELSVRIKNLLEQRNRLKEKFGKELKINPENVTVNFVDKEFLERALKIAEN
                     NIYSSDFDSELFAKEMFVSRSQLHRKMIAITGQAPGEFIRIYRLKKAAQLLLEKKLSV
                     TQISLEVGFNSPSHFTKAFQQYFNCLPSEFVGPK"
BASE COUNT          961 a          503 c          468 g         1111 t
ORIGIN      
        1 gttctgaggc aaagaaagca caattggctg aggtgtaggg gcattatcaa taaggatatc
       61 atcaatatag tatgtacctc cactatatcc ataacatccg atacgaatac gggcatttgc
      121 ctgacggaaa ttacctaaag aaacctgtaa acgattatac agtgtgccac taaagtacgg
      181 ttgccatatt gttgaccagg tatttccgcc atcatttgaa gcttcaatac ttacgtaacc
      241 accaccaccg tctgcttttc taacccaaaa agaaaggtag ggattgggtg ctgatgaaag
      301 atcaatattt gatgctaaag ttagataatt gtacgaacca ttgaatggca acattgccca
      361 aacctgggta ccgctatgcg catttgtaat ctgtattttc cactgtagtg tatctctatc
      421 ccaatatgca gatgatgttg cgtttgtttc aacatcgtca taaaacggga aggcaacttg
      481 aacattattt gcagatgagg gtgagtttga ttgtgcaacg gttaaaaaat gcaacagcag
      541 agtaagtgcc gcaatcattg aaaaaagttt tttcattttg tatttctcct tttttgattt
      601 aattggtatt gtttattaac tgcaagaaca agcatcatta aattcgattt ttataatatg
      661 agtaatattt tattgtttga taaaatctta atacttttta gaattataag gctattaaac
      721 tgaaaataat taacaataca caaatactga cgataatgaa taaaccatag agggctattg
      781 aaaaaagccg ttcttgtttt tttatttctt cgtcagtttt ttgcattttt taggataatc
      841 atcgttttta ttaaatacga ttttgatcaa tcctgattag tgttacagaa ttttatataa
      901 caccactgga tcagtggata taatcaattt tttattttaa tcggattagt tattaaaata
      961 tcttaatcaa atttaatata actgattggg ggcagatggc gattttatgc caatctttag
     1021 cggagttgtt gcaaagtagt cgaaatcttg ccgaattgac gtaaaattac tttggtccca
     1081 cgaactctga tggcagacaa ttaaaatact gctgaaacgc ttttgtaaaa tgtgaaggac
     1141 tgttgaaacc tacttctaat gaaatttgag taacacttaa ttttttttct aaaagtaatt
     1201 gtgcggcttt tttcaatcta taaattcgta taaactcacc cggagcctga cctgtgattg
     1261 caatcatttt tcgatgaagt tggcttcgac ttacaaacat ttcttttgca aataattctg
     1321 agtcaaaatc tgagctgtaa atattatttt ctgcaatttt taatgctctt tctaaaaatt
     1381 ccttatcaac aaaattaaca gtaacatttt ccgggttaat tttaagttct ttaccaaact
     1441 tttcctttaa tctgtttctt tgctctaaaa gatttttaat gcgaactgaa agttctttaa
     1501 agttaaatgg ctttgtaaca taatcatctg cgcctgtttc taatccttca atcttatttt
     1561 cagaagatac ttttgctgtt aataatataa tcggaatatg gctagtctga aaatttgttt
     1621 ttatctgctt gcaaaattct aaacctccca tacccggcat cataacatcg cttaaaatta
     1681 aatcaggcat ttgttctgat gctttaataa ttccctcttc tgcattcatc gcctgaatta
     1741 ttttgtaatc attctggaat aaagaataaa tatattcttg tacatcgctg gaatcttcaa
     1801 caactaatat tgaagggtta ttagtcgtca atttatcatc agaaatattt tggtctttta
     1861 ctgcattatt ggattctgtg ataacatctt gattgtaaga tttatctgtt actgcttcta
     1921 gaatatcttc agatcgaatt tcatctttta ttttttgatt atctgataaa taatattcac
     1981 ttagaggaat agttaagata aaagttgtcc ccttttctat ctcgctttca atattaattt
     2041 cccatttgtg cagatcaatt aattctttaa caagtgctaa tccaattccg gagcctccat
     2101 aaactcttcc ggaagaatca tcaacctgat aaaaacgatc aaatatttta tgaagcttat
     2161 cttcaggaat tccaattccg gtgtctgata tttttattct cgcaaattct tcatttcctg
     2221 taattatttt ttttactgct aaagatatta tcccaccttc agctgtaaat ttaaaagcgt
     2281 ttgaaagaat attattaatg attttttcaa gtttatctct atctacccat gcgcatatag
     2341 atttatcatc ggatacaaat tcaattgaaa tatttttttc atttgcaatt gattcaaaag
     2401 agaagaatat accacgaagt aatgtgatta aattgacttc gcgtgctttt acagggatcg
     2461 cttcggcttc caattgagtc agttcaagta attggtcaat caaagtatgt aaattttccg
     2521 tgttacggta aataatatca taataatttt gcggatcacc tttaacccgt ccattcttta
     2581 gttgttctaa tggacctttg atgagcatta atggagtgcg gaattcgtgc gaaagattgg
     2641 caaaaaatct ggattttaca ttttcaatat ctcgatgttt ttttgattca aaatcgcgta
     2701 tttttatttc atgacgtagc ttgataagat ttagctcgaa tcggcgaatt cctaccaatc
     2761 ctaaaaatat aactaatata taaactgaat aagcccagcc agtcttccac caaggtggat
     2821 ttattgtgac ttttattgat ttaacagtct taccccaaat gccatcgctg tttgtagctt
     2881 ttaatctaaa ataatatgtg ccaggattta gattattata cgctgcaaat cttcttttag
     2941 tggaataaat ccaatcttta tcaaatcctt caagtctata caaaaaatga atgtgttctg
     3001 atgaattaaa atctaaggat gaaaaattaa aagttatatt att
//
LOCUS       DASWBY010000581        12236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_661309, whole genome shotgun sequence.
ACCESSION   DASWBY010000581 DASWBY010000000
VERSION     DASWBY010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 12236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12236
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661309"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1996)
                     /locus_tag="VF870_10500"
     CDS             complement(<1..1996)
                     /locus_tag="VF870_10500"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX9252663.1"
                     /translation="MNQKKYTLKRLGQTGLVQPTDEARFTINYQEELNSSQFEAASAV
                     NGCYLIIAGAGTGKTRTLVYRVARLIELGHDPNSILLLTFTRKAANEMMKRASMLLDD
                     RCSKIRGGTFHSFANLTLRKYAKAIGLDSSFTILDQGDSADVINLIRSQDGFITKEKR
                     FPNKQTLNKVFSLSVNTGKKIEEIIQNDYPHFIPLLDKILEIQKIFIEYKRRNNLLDY
                     DDLLVYLREFLRKGGPAVKALLSEIKFVMVDEYQDTNHLQSEIVKGLSQYSKNVMVVG
                     DDSQAIYSFRGADFHNIMQFPKLFDDVKIIKLEENYRSHQSILDFANRINDAAVEKYK
                     KDLYSRRGSGQLPNIVAASTENLQSKFIVEKILDLREEGVPLKDISVLFRSSFFSFDL
                     ELELAKANVPFQKFGGMKFVETAHVKDVMAFLRIVINPKDVISWYRVLLLHEGVGPKT
                     AQRILDELATSRITIKAEPDQQPEFKHHKLGPLFYLLYELQKKKYIPSEMVQKVYDYY
                     WDLFKANYDDWNKRKKDLEIFQNIVENYTSVETLLSDMAIEPIIESVVDVDATDKEEE
                     YVTLSTIHSAKGLEWHSVFIIHAVEGYFPSARSVESLDTLEEERRLMYVASTRAKNNL
                     FVTYPMNLYDREAGMTLSKPSRFIADITPDLAEGWLLEEDF"
     gene            complement(1993..2607)
                     /locus_tag="VF870_10505"
     CDS             complement(1993..2607)
                     /locus_tag="VF870_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252664.1"
                     /translation="MDDLFQYLIWGIIIFSFISSFFKKKEPPKQTPLPKAKQPDVVNY
                     PKQQTDVERITVSQKQDEYDILRELENLFKGEIKTPTPQKQKQGDPYDIYESNEIKDK
                     DLDLVVDKRMQRNVEDMKPIGSRETYDNKSAGRRSVSDRQAQQKIINPKIEEQAKEFE
                     RVLAGPKQQRATVTEFNRKLKNPRTVKEYILFSEILGKPKALRR"
     gene            complement(2662..3651)
                     /gene="floA"
                     /locus_tag="VF870_10510"
     CDS             complement(2662..3651)
                     /gene="floA"
                     /locus_tag="VF870_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561106.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HEX9252665.1"
                     /translation="MEALTGLSIVIIIAVALFLILFLYFVPLGLFITAYFSGVKVKIV
                     RDLIGMRLRKVPPVLIVRNMITVTKAGLTVDQAKLEAHYLAGGNVTKVINALISADKA
                     NLGLTFERATAIDLAGRDVLEAVKMSVVPKVIETPMVSAVAKDGIQLKAMARVTVRAN
                     IERLVGGAGEATILARVGEGIVSTIGSSASHKDVLENPDKISKSVLAKGLDSGTAFEI
                     LSIDIADVDIGQNIGAILQADQAEADLKVARAKAEERRAAAVATEQENIAEVAKMKAK
                     VVEAEAEVPRAISDAFRNGKLGVMDYYNLKNVQADTEMRTSIAQPEEKKKTDS"
     gene            3989..6496
                     /locus_tag="VF870_10515"
     CDS             3989..6496
                     /locus_tag="VF870_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX9252666.1"
                     /translation="MDGNFSDRLQDVIRLSREEALRLGHDYIGTEHLLLGIIREGQGV
                     AVRILRNLDCDLVKLKKAIEDTVRTSGGTLTIGNIPLTKQAEKVLKITQIESKIYKAD
                     VIGTEHLLLSLLRDEDNIATQILHQFNVTYDSARSELNSMLSAKNPKDANQKVPPMMD
                     RKTDKTKTPVLDNFGRDLTKLGSEDKLDPVVGREKEIERVAQILSRRKKNNPVLIGEP
                     GVGKTAIAEGLALRIIQKKVPRTLQDKRVVTLDLAGLVAGTKYRGQFEERMKALMNEL
                     EKAEDVILFIDELHTIVGAGGASGSLDASNMFKPALARGDIQCIGATTLDEYRKFIET
                     DGALDRRFQKVMVDPPTYDETLQILENIKFKYEEHHRVRYSKEAIQTAVKLSNRYITD
                     RHLPDKAIDVIDEAGSRVHMGNFEVPQDILDLEEEIEGVKKDKARVVKMQDYEEAARL
                     RDKERNLLSDLETAKREWDTKTKDIVHDVSEEDIATVVAMMTGIPVNRIAQTESEKLL
                     KMADALKKSIVGQDEAVEKLSKAIRRTRAGLKSPNRPIGSFIFLGPTGVGKTELCKVL
                     AKYLFDSEDALVRIDMSEYMEKFSVSRLVGAPPGYVGYEEGGQLTEKVRRKPYSVVLF
                     DEIEKAHPDIFSLLLQVLDDGTLTDSLGRKVDFKNTIIIMTSNVGTRDIKNISKFGFG
                     GDDKADKYSSLKDTVEEAMKRLFNPEFLNRIDDTIVFRNLDKEDIYKIIDIEIKDLLA
                     NIRENKLTLVLDESAKEFLVNKGFDEKFGARPLRRALQKYVEDPLAEEILRGSFKEGT
                     TIISKHVENSEDLVFFDESLEHPDPNVEPEKTDLGEN"
     gene            6593..6877
                     /locus_tag="VF870_10520"
     CDS             6593..6877
                     /locus_tag="VF870_10520"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX9252667.1"
                     /translation="MSKLNQEQINSNLSKFSGWAFENESISKQFQFKDFIEALSFVNA
                     VGLEAEKMDHHPDILMFAWNKVKITISTHSAGGVTENDFSLAQKIEERKK"
     gene            6874..7185
                     /locus_tag="VF870_10525"
     CDS             6874..7185
                     /locus_tag="VF870_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252668.1"
                     /translation="MSTRLDELFDKYDENPLDPDIIFSIVKEYLKNNEDEEAEVYLKD
                     LIKKDPKYVQAYISYGNLKENHNEIEDAEYYYRTGLRIAKELNDTKAVRVLEDYLDEL
                     E"
     gene            7172..7756
                     /gene="pyrE"
                     /locus_tag="VF870_10530"
     CDS             7172..7756
                     /gene="pyrE"
                     /locus_tag="VF870_10530"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX9252669.1"
                     /translation="MNLSNEDVIKIFKSTEALLEGHFLLTSGRHSNQYFQCAKVLQYP
                     EYTSQICSLITNHFKDKEIDTVIAPAMGGMIIGYEVARQLNKRSIFTERENKEMTLRR
                     GFSLSKGEKVLVCEDVVTTGGSVFEVIEIVKKFGAEIVGVGSIVDRSNGKVDFGYDFF
                     PSLRLNAVSYLPEECAICKEGKLELVKPGSRKAF"
     gene            7876..9147
                     /locus_tag="VF870_10535"
     CDS             7876..9147
                     /locus_tag="VF870_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HEX9252670.1"
                     /translation="MKKTLFAILIISNLIFSQIKFDQYFENKTLRLDYFHTGNDTSDS
                     YSFDEVIAEPYWGGPQTNMLDKFNYGKYKFIIKDFDSGTEIYSKTYSTLFSEWQTTDE
                     AKQTIKSFSETVVFPYPKNSVTVEFYSRDKKNILHKKFEYVVNPKSYFIKTERALEYK
                     NFEVINNNDPGKAVDIVIIPDGYTKDEMEKFRNDCEKFAGYLFNSSPYKENKNKFNIW
                     GIEAPSKESGTDIPAESIWKNTIINSQFYMFDLERYLMTSDNKSLRNIASNAPYDQIF
                     ILVNTNKYGGGAIYNHYSVCVSDNNFGEYIFTHEFGHGFAGLGDEYYTSDVAYNEFYP
                     LDVEPLDPNLTTLVNFDSKWKNMVDTNTPIPTPATKEFKNTVGVYEGGGYVAKGVYRP
                     MQDCSMKSISVDNFCPVCKQAIEQMIDFCSE"
     gene            9163..10395
                     /locus_tag="VF870_10540"
     CDS             9163..10395
                     /locus_tag="VF870_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9252671.1"
                     /translation="MDQKKLHKTVETIASQKFATDEEMLTTVVNEIVHDQNTGFTGGR
                     IWKLYPDLEGYRILYQTGKMEKIDKNFTIRALEYPVFEQLAQSRTILGHETIEALRKK
                     GIFKYSASGVGHKTKIQGKPFYEYVLALNSKNIDEDLRINLSIIATALTSQIKQRKIS
                     HSANLLKADIDKARQLQKSILPEHEYKFHHFDIYGITDPAEIVGGDFFDYLDIGDNND
                     RIGVAIGDAASKGVAAAAEAMYVSGALRMATTFEIKISLILKRMNELVNKIFEDDKFT
                     SLFYGELSTYKNGLFLYANAGHNPPIFYRSAQNKFQYLQPTGPVLGPAPQSKYYVENI
                     NIYLNDILVLYSDGVTEAANTKFAFYGEQKLLSIIRKLKDKTPKEIALGILEDVIKFS
                     KNGSYTDDKTIVVIKRTS"
     gene            10563..11927
                     /locus_tag="VF870_10545"
     CDS             10563..11927
                     /locus_tag="VF870_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9252672.1"
                     /translation="MLTSINPANNKTIKTYDEMNSGESKKTISLADDEFILWKETTFK
                     HRSDLMRNAAEVLRDNSEEYSVLMTMEMGKPIVQSRAEVEKCAWVCDYYADNAENFLS
                     DEIIKTEATKSFVSYQPLGVILAIMPWNFPFWQVFRFAAPNLMAGNAGLLKHASNVSG
                     CALAIEDVFRKAGFPDNLFRTLLVSSKNIKDVIANEKVQAVTLTGSVPAGKSVASLAG
                     SLIKKTVLELGGSDPYIILEDADLEKAAMSCVTSRLINAGQSCIAAKRFIVVESVYDE
                     FEKLFLEIMSKKKMGDPLDIKNDLGPQASLSLRDELYEQVLRSVKQGAEIIFGGTIPE
                     INGAYYPPTILKNVKPGIAAFDEELFGPVAALIKAKDENEAINLANQSIFGLGAAVFT
                     KDVNRGEQLAKEKLKAGCCFVNDFVKSDPRLPFGGIKESGYGRELSPFGIKEFVNIKT
                     VYVK"
     gene            complement(11928..>12236)
                     /locus_tag="VF870_10550"
     CDS             complement(11928..>12236)
                     /locus_tag="VF870_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9252673.1"
                     /translation="PMFLTDVEGKIIDANKEFIYFLGYAIYDLKRMTIKSIVPMNNHE
                     NETKILLEELEINKHISSRWCEFFKINGEIIQAELEVFATFDENNRFDGITRIVKNIL
                     "
BASE COUNT         4148 a         1988 c         2219 g         3881 t
ORIGIN      
        1 aaaaatcctc ttccagtagc cagccttcag ctaaatcagg agtaatatct gcaatgaaac
       61 gagatggttt tgataatgtc atacctgctt cacgatcgta aaggttcatt gggtaagtaa
      121 caaacaaatt attttttgca cgggtagagg caacatacat caatcgtctt tcttcttcca
      181 gggtatctaa actttcaaca gatcttgcag atgggaagta tccttcaact gcatgaatta
      241 taaatacgga atgccactcc aatccttttg ctgaatgaat ggtagaaagc gttacgtact
      301 cttcttcttt atcagtagca tcaacatcaa caacactttc tataattggc tcgattgcca
      361 tatcagaaag taaagtttca acagaagtat aattttccac aatgttctgg aaaatttcta
      421 aatccttttt acgtttattc cagtcatcgt agttagcttt gaataaatcc caataataat
      481 cgtaaacttt ctgcaccatc tcagaaggaa tatatttttt cttttgaagt tcatataaga
      541 gataaaacaa cggaccaagt ttatgatgtt taaattcagg ctgctggtca ggttcagctt
      601 taattgttat tctggaagtt gcaagttcat ctaaaatacg ttgtgcagtt tttgggccaa
      661 cgccttcgtg taatagtaaa actctgtacc agctaataac atctttggga ttaataacaa
      721 tccttaaaaa tgccattaca tcttttacat gagcagtttc aacaaacttc attccgccaa
      781 acttttgaaa aggaacattt gcctttgcaa gttcaagttc aagatcaaaa gaaaaaaaag
      841 aagaacgaaa caatacagaa atatctttta atggaacgcc ttcttcccta agatcaagaa
      901 ttttttctac aataaatttt gattgaagat tttctgttga agccgctaca atgtttggta
      961 actgtccgct gccacgtctt gaataaagat cttttttata cttttcaact gcagcatcgt
     1021 taattctatt tgcaaaatca agtatcgatt gatgacttct ataattttct tcaagtttaa
     1081 taatttttac atcatcaaat agttttggga actgcataat gttatggaaa tcagcaccac
     1141 gaaaagaata aatcgcctgt gaatcatctc ccacaaccat aacattttta ctgtattgcg
     1201 ataatccttt tacaatttct gattgtaaat gatttgtatc ctgatactca tcaaccataa
     1261 caaatttaat ttcggacaga agcgctttaa cagcaggacc acctttacga agaaactctc
     1321 gcaggtaaac aagaagatca tcataatcca gaagattgtt acgtcgttta tattcaataa
     1381 atattttttg gatttctaaa atcttatcaa gcaatgggat aaaatgcgga taatcattct
     1441 ggataatctc ttcaattttc tttccagtat taacacttag actaaaaact ttattaagtg
     1501 tctgcttatt aggaaatctt ttctcctttg tgataaatcc atcttgagaa cggataagat
     1561 ttataacatc ggcgctgtca ccctgatcaa gtattgtgaa agacgaatca agtccgattg
     1621 ctttagcata ttttcttagt gtaagatttg caaacgaatg aaaggtgccg ccacgaattt
     1681 tagaacagcg atcatcgagt agcattgatg cgcgtttcat catttcattt gcggctttgc
     1741 gagtgaatgt taaaagcaga attgagtttg ggtcgtgacc aagttcaatt aatcttgcaa
     1801 cacgataaac taatgtgcgt gttttgcctg ttccggcacc tgcaataatt aaataacatc
     1861 cattaacggc ggaagccgct tcaaactgag aagaatttaa ttcttcctgg taattaattg
     1921 taaaacgggc ttcgtccgtt ggttgaacta agcccgtttg tccaagtctt tttaacgtat
     1981 attttttctg gttcatctgc gcagagcttt tggttttcct aaaatttctg aaaataaaat
     2041 atactctttt accgttctgg gattttttaa tttacggtta aattcagtta ctgttgccct
     2101 ttgctgcttt ggcccggcta aaacccgctc aaattcttta gcctgctctt ctattttggg
     2161 atttataatt ttttgttgtg cctgcctgtc agatacagat cttctgccag cacttttatt
     2221 atcataagtt tctctggaac caataggctt catatcctct acatttcgct gcattctttt
     2281 atctacaaca agatcaagat ctttatcttt tatttcgttt gattcgtaaa tatcatatgg
     2341 atcaccctgt ttctgttttt gcggggtagg agttttaatc tcacctttaa agagattttc
     2401 tagctctcgc aaaatatcat attcatcctg cttttgcgaa acagtaatac gttcaacatc
     2461 agtttgctgt ttaggatagt taacaacatc cggctgtttt gcttttggca aaggagtttg
     2521 cttcggcggc tctttctttt taaagaaaga gcttatgaag ctaaaaatta ttatacccca
     2581 aatgaggtat tgaaataaat catccataag gcttctctat atttcttttt aattattctt
     2641 tatattcatt ttagattcgc cttatgaatc agtttttttc ttttcttccg gttgtgcaat
     2701 cgatgttctc atttcagtat cagcctgaac attttttaga ttataataat ccataacacc
     2761 aagtttacca tttctaaatg cgtctgaaat tgctcttggt acttcagctt cagcctcaac
     2821 aactttagct ttcatcttgg caacctcagc aatattttcc tgctcggttg cgactgcagc
     2881 tgcacgtctc tcttctgctt ttgcacgagc aacttttaaa tctgcttctg cctgatcagc
     2941 ctgaagaatt gcgcctatat tctggccaat atctacatct gcaatatcaa ttgatagaat
     3001 ttcgaaggcg gtaccactat ccagaccttt tgctaaaact gattttgata tcttatccgg
     3061 attttcaaga acatctttat gagaagcgct tgatccgatc gttgaaacaa ttccttcacc
     3121 aactcttgca agaatcgttg cttcaccagc gccaccgact aatctttcaa tatttgctcg
     3181 gacggtaacc ctagccatag cttttaattg aataccatct tttgcaacag cagaaaccat
     3241 cggagtttca attacctttg gaacaacaga cattttaact gcttcaagca catctcttcc
     3301 tgcaaggtca attgctgtag cacgttcaaa tgttaatcca aggttagctt tatctgcaga
     3361 aattaatgcg ttaattactt tggtaacatt tccaccggca aggtaatgcg cttcaagttt
     3421 tgcctgatct actgtcaaac cagctttagt aaccgtaatc atatttctga caattaatac
     3481 cgggggaact tttcttaatc tcattccaat taaatcacga actattttta cttttactcc
     3541 tgagaagtaa gcagtaataa ataatccaag tggaacgaaa taaagaaaga gaattaagaa
     3601 taaagcaacc gcaattatta ttacgattga taatcctgtt aatgcttcca tctatactcc
     3661 ataattttgt gtaataaaat aaatattgtg cagatttgaa tctgcaaatt ttcaaatatc
     3721 tgccttaatg actgttatta agttttaatg acgaataatc attcatttta gcagaacttt
     3781 tttctttctg atctgaaatt atagaaaatt ttaaccaaat gtactggcat ttgaattgaa
     3841 ctatgtaaat cttatcattt cagaattata tttaacaaat cattgattta aggctggctt
     3901 gatataggat agcacttatt gttatatttc aattgtgaaa cgaatttaga gaaaaatttg
     3961 ttttactttt aatcaaataa ggagatatat ggacggaaat ttctcagata gattgcagga
     4021 tgtaattaga cttagcaggg aagaggctct tagacttggg catgattata taggaacaga
     4081 acatctgctg cttggaatta ttcgtgaagg ccagggagtt gctgtaagaa ttctaagaaa
     4141 tcttgattgt gatcttgtaa aacttaaaaa agcaattgag gatacagtta gaacttccgg
     4201 cggaacttta acgattggaa atattccgct aactaagcag gcagaaaaag ttttaaagat
     4261 tactcagatc gaatcaaaaa tttataaagc tgatgttata ggaacagaac atttgctttt
     4321 atcattgtta agagatgaag ataacattgc aacacaaata cttcatcagt ttaacgttac
     4381 ttatgattca gcacgatcgg aacttaattc tatgttaagt gctaaaaatc caaaggatgc
     4441 taatcaaaaa gtaccaccga tgatggatag aaaaaccgat aaaaccaaaa ctccagtact
     4501 ggataatttt ggtcgggatc ttactaaact tggatctgaa gataaacttg atccggttgt
     4561 tggcagagaa aaagaaattg aacgcgttgc acaaattctt agcagaagaa aaaagaacaa
     4621 tccggtttta atcggcgaac ccggtgttgg taaaactgca attgctgaag gtttggcact
     4681 tcgtatcata cagaaaaaag ttccaagaac tttgcaggat aaacgtgttg ttactttaga
     4741 tcttgccgga cttgttgccg gaacaaaata ccgcggacag tttgaagaga gaatgaaagc
     4801 tttaatgaac gagcttgaaa aagccgaaga tgtaattctc tttatcgatg aacttcatac
     4861 aatagtgggt gcaggtggtg ctagcggctc acttgatgct tcaaatatgt ttaaacctgc
     4921 cttagcacga ggggatatac aatgtattgg tgcaacaacg ttggatgaat acagaaaatt
     4981 tatcgaaact gatggtgcac ttgatcgtag atttcagaaa gttatggttg atcctccaac
     5041 ctatgatgaa acacttcaga ttcttgaaaa tataaaattc aaatatgaag aacatcatcg
     5101 cgttcgatac tcaaaagaag caatccaaac agcggttaaa cttagtaatc gttacataac
     5161 agatcggcat cttccggata aagctatcga tgtgatcgat gaagcgggct caagagttca
     5221 tatgggcaac tttgaagtac ctcaggatat tcttgatctt gaagaagaga ttgaaggtgt
     5281 aaaaaaagat aaagcacgcg ttgttaaaat gcaggattat gaagaagctg caagactgcg
     5341 cgataaagaa cgcaatcttt tatctgatct tgaaactgca aaacgtgaat gggatacaaa
     5401 aacaaaagat atcgttcacg atgttagcga agaagatata gcaactgtag ttgcgatgat
     5461 gacgggcata ccggttaatc gtattgctca aactgaatct gaaaaactct taaagatggc
     5521 tgatgcttta aagaagtcta tcgttgggca ggatgaagct gttgaaaaac tttctaaagc
     5581 tattcgcagg acaagagcag gattaaaaag tccgaatcgt ccaataggaa gttttatttt
     5641 cttaggacca accggagttg gtaaaacaga attatgtaaa gtgcttgcaa aatatttgtt
     5701 cgacagcgaa gatgcacttg taagaattga tatgagtgag tacatggaaa agttttctgt
     5761 ttcaagattg gttggagcgc ctcccggtta cgttggatat gaagaaggcg gacagttaac
     5821 tgaaaaggta agacgtaaac cgtattcagt tgttctgttt gatgaaattg aaaaagctca
     5881 tccggatatt tttagtctac tgcttcaggt tcttgatgat ggtactttaa ctgattctct
     5941 cggcagaaaa gttgatttca aaaatacaat catcattatg acctcaaacg ttggaaccag
     6001 ggatataaaa aacataagca aatttggttt tggcggagat gataaagctg ataagtattc
     6061 cagcttaaaa gatactgttg aagaagcaat gaagagattg ttcaatcctg aattcctaaa
     6121 cagaattgat gatacaatcg tatttagaaa ccttgataaa gaagatattt acaagattat
     6181 agatatagag attaaagatt tacttgcaaa catcagagaa aacaaattaa cacttgtatt
     6241 agatgaatca gcgaaagagt ttttagttaa caaaggtttt gatgaaaaat ttggtgcccg
     6301 tccattaaga agagcgcttc aaaaatatgt tgaagatcca ttagctgaag aaatattgcg
     6361 cggttcattc aaagaaggaa caacaataat ttctaagcac gttgaaaatt ccgaagactt
     6421 ggtttttttt gacgaatcat tggaacatcc tgatccaaat gtagaaccgg aaaaaacaga
     6481 tttgggtgaa aattagttca tcatttaatt agacctttca taagcgcatt ctttaggatg
     6541 cgcttttttt tgtttatttt tgttccagaa taattaaaac ctcaggtaaa agatgagtaa
     6601 gttgaatcaa gaacaaatca attctaatct ttcaaagttt tctggatggg cttttgaaaa
     6661 tgaatcaatc tctaaacaat tccagtttaa agattttata gaagcattat catttgtaaa
     6721 tgctgttggt ttagaagctg aaaaaatgga tcatcatccg gatattttaa tgtttgcctg
     6781 gaataaagtt aaaattacaa tttcaaccca cagtgcaggt ggagtaacgg aaaatgattt
     6841 ttcacttgcg caaaaaatag aggaaagaaa aaaatgagta cgcggctcga tgaacttttt
     6901 gataaatatg atgaaaatcc attggacccg gatataattt tcagcattgt aaaagagtac
     6961 ttaaaaaata atgaagatga agaggctgag gtatatttga aagatttaat caaaaaagat
     7021 ccaaagtatg tgcaggctta catttcttat ggcaatctta aagaaaacca taatgaaatt
     7081 gaagatgctg agtattacta ccgcacagga ttacgcatcg ctaaagaatt aaatgacaca
     7141 aaagccgtaa gagtattgga ggattatctc gatgaacttg agtaatgaag atgttataaa
     7201 aatatttaaa agcaccgaag ctttattgga aggacatttc cttttaactt cgggaagaca
     7261 cagcaatcaa tattttcaat gtgcaaaagt tttgcagtat ccggaatata cttcccaaat
     7321 ttgttctctt ataacaaatc attttaaaga caaagagata gatacagtta ttgctccggc
     7381 aatgggtgga atgataatcg gttatgaagt tgcaagacaa ttaaataaaa gatccatttt
     7441 tacagaaaga gaaaataaag aaatgacttt acgccgagga ttttctttat ccaaaggtga
     7501 aaaagtttta gtctgcgaag atgttgttac aaccggcgga tcagtatttg aagtgattga
     7561 aatcgttaaa aagtttggag cagaaatagt tggagtagga agtattgttg accgtagtaa
     7621 tggtaaagtt gattttggtt atgatttctt tccttcactc cgattgaatg ccgtttcata
     7681 tctgccggaa gaatgtgcga tatgtaaaga aggtaaatta gaattagtaa aaccgggttc
     7741 aagaaaagct ttttagcatt gtcgttatta acactcttat aaaattccat gtcacactga
     7801 gcctgtcaaa gtgtggcaat tgatatactg tcagtcttcg acgggctcag actgacaaat
     7861 tttgaattat actatatgaa gaaaactctg tttgcaatcc ttataatttc aaatttaatt
     7921 ttttcacaaa taaaatttga tcagtacttt gagaataaaa cattaaggct ggattatttt
     7981 cacaccggca acgatacaag cgattcatat tcatttgatg aagttatagc agaaccttat
     8041 tggggcggac ctcaaacaaa tatgcttgat aagtttaatt atggcaaata caagtttatt
     8101 ataaaagatt tcgattcagg aacagaaatc tattcaaaaa catattcaac attattcagt
     8161 gagtggcaaa cgactgatga agcaaaacaa acaattaaaa gttttagtga aacagtagtg
     8221 tttccatatc caaaaaattc agtgacagtt gaattttact ccagggacaa aaagaatatt
     8281 ctacataaga aatttgaata tgttgtaaat cccaaaagtt attttattaa aactgaaaga
     8341 gcgcttgagt ataaaaactt tgaagtaata aataataatg acccgggtaa agctgtggat
     8401 attgttatca ttccggatgg ctatactaaa gatgaaatgg aaaagttcag aaacgattgt
     8461 gaaaaatttg caggatattt atttaattca tcaccttaca aagaaaataa aaacaaattt
     8521 aacatttggg gaattgaagc tccatccaaa gaatctggaa ctgacattcc tgctgaaagt
     8581 atctggaaga acactattat taactcgcag ttttatatgt ttgaccttga gagatatttg
     8641 atgacgagtg ataataaatc tttacgtaac attgcctcca atgcacccta tgatcagata
     8701 tttattttag ttaacactaa taagtacggc ggcggagcaa tttataatca ttattctgtt
     8761 tgtgtcagcg ataataattt tggtgaatat atttttacac atgaatttgg gcacggattt
     8821 gccggactag gtgatgagta ctacacgtca gatgttgctt ataatgagtt ttatccactc
     8881 gatgttgagc cgctcgatcc taatttgaca acactggtta attttgattc aaagtggaaa
     8941 aacatggttg atacaaatac accaatccca actccggcaa ctaaagaatt taaaaataca
     9001 gtaggtgtct atgaaggcgg cgggtacgtt gctaaaggag tttacagacc gatgcaggat
     9061 tgttcaatga aatcaatttc tgttgataat ttttgtcctg tgtgtaaaca agcaatagaa
     9121 caaatgatag atttttgtag tgaataatta ataaggaata ttttggatca aaaaaaatta
     9181 cataaaactg ttgaaacaat tgcatcgcaa aagtttgcta ctgatgaaga aatgttaaca
     9241 acagttgtaa acgaaattgt tcatgatcaa aacacaggtt ttacaggcgg gagaatttgg
     9301 aaactttatc ctgatttaga aggatacagg attctttatc agactggtaa gatggagaag
     9361 attgataaaa attttaccat tcgtgcttta gaatatcctg tctttgaaca acttgctcaa
     9421 tccagaacga ttctcggcca cgaaacaatt gaagcattaa gaaaaaaagg gatctttaaa
     9481 tattctgctt caggagttgg gcataaaaca aagatacaag ggaagccttt ttatgaatat
     9541 gtgctggcgc tgaattcaaa aaacattgat gaagatttaa gaataaactt aagcatcatt
     9601 gccacagcac tgacatcaca gattaagcag agaaaaattt ctcacagcgc taatttatta
     9661 aaagccgaca ttgacaaagc aagacagctt caaaaaagta tcttgcccga acacgaatat
     9721 aaatttcatc attttgatat ttatggtatt actgatccgg ccgagattgt tggaggagat
     9781 ttttttgatt acttagatat tggggataat aatgatagaa tcggagttgc aattggtgat
     9841 gcggcaagca aaggtgttgc agctgctgca gaggcaatgt acgtctcagg ggcgttgcgt
     9901 atggctacta cttttgaaat aaaaatctca ctcatcctta aacgaatgaa cgaacttgtt
     9961 aataaaatat ttgaggatga taaattcact tcattatttt atggtgaact ttctacatac
    10021 aaaaatggat tgtttttata cgctaatgcc ggacacaatc cgccaatatt ttaccgaagt
    10081 gcacaaaata aatttcaata tcttcaacca acaggaccag tactcggacc agccccgcaa
    10141 tcaaaatatt atgttgaaaa tataaatata tatttaaatg atatactggt tctatattct
    10201 gatggagtta ctgaagcagc taacacaaag tttgcatttt acggcgaaca aaaattgtta
    10261 tcaataatca gaaagctaaa agataaaact ccaaaggaaa ttgcactcgg tattttagaa
    10321 gatgtaatta agttttctaa aaacggttct tatacagatg ataaaactat tgtggtgata
    10381 aagagaactt cttagataaa tattttacta aattttgcac ataattatcc ttttagaacc
    10441 agccgcttat tggcaagttc taagctaata caattttata gttagaagtc actcttcgac
    10501 aagctcagag tgacgatatg atttgaatta aaatttattt tattaataaa tctttaaata
    10561 ttatgctaac ctcaatcaat cctgcaaata ataaaacaat caaaacttac gatgaaatga
    10621 attctgggga atctaaaaag accatttcgt tagcagatga tgaatttatt ctttggaaag
    10681 aaacgacttt taaacacaga tcagatttga tgagaaatgc tgcagaagtt ttaagagata
    10741 attctgaaga atattctgtt ttgatgacca tggaaatggg caaaccaatt gttcaatcaa
    10801 gagcagaagt tgaaaaatgt gcgtgggttt gtgattatta tgcagataat gctgagaact
    10861 ttttatctga tgaaattatc aaaacagaag caactaaaag ttttgtatca tatcaaccgc
    10921 ttggagttat acttgcaatt atgccatgga attttccttt ctggcaggta tttcgttttg
    10981 ctgctccaaa tttaatggca ggcaatgcag gattgttaaa acacgcctct aatgtttccg
    11041 ggtgtgcttt agctattgaa gatgttttta gaaaagccgg atttccggac aatctttttc
    11101 gcacattatt ggtgtcatca aaaaatatta aagacgttat tgctaatgaa aaagtacaag
    11161 cggttacttt aaccggaagc gttcctgcag gaaaatcagt tgcatctctt gccggcagtc
    11221 tgattaaaaa aacagtttta gaattgggcg gaagcgatcc ctatattatt cttgaagatg
    11281 ctgacttaga aaaagctgca atgtcttgtg taacctcaag attaattaat gcaggtcaaa
    11341 gctgtattgc tgcaaaaaga tttattgtag ttgaaagtgt ttatgatgaa tttgaaaaac
    11401 tatttcttga aattatgtct aagaaaaaaa tgggtgatcc tttagacatt aaaaatgatt
    11461 tgggtcccca agcaagttta tctttgcgag atgaattgta tgaacaggtt ttaagaagtg
    11521 taaagcaagg tgctgaaata attttcggcg gaactattcc tgaaattaac ggcgcttatt
    11581 atccgccaac aattttaaaa aatgtaaaac ctggaatagc agcctttgat gaagaattat
    11641 ttggtccggt tgcggcattg ataaaagcaa aagatgaaaa tgaggcgatc aatcttgcaa
    11701 accaaagtat ctttggttta ggtgctgcag tttttacaaa agatgtaaac aggggagaac
    11761 aattagcaaa agaaaagctt aaggcaggat gctgctttgt aaatgatttt gtaaaatccg
    11821 atccaagact accatttgga ggaattaaag aatcaggtta cggcagagaa ctttctccat
    11881 ttggaataaa agaattcgta aatatcaaaa ctgtttatgt taaataatta caaaatattt
    11941 tttactattc ttgtaattcc atcaaaccgg ttgttctcat caaaagttgc aaaaacttca
    12001 agctctgcct gaattatttc tccattgatt ttaaaaaatt cacaccagcg cgaagagata
    12061 tgtttattta tctcaagttc ttcaagcaat attttggttt cattttcgtg gttgttcatt
    12121 ggtacgatgg atttgatggt cattcttttc aaatcataaa ttgcataacc caaaaaatat
    12181 ataaactctt tattggcatc aattattttc ccctcaacat ctgtaagaaa cattgg
//
LOCUS       DASWBY010000582         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_285728, whole genome shotgun sequence.
ACCESSION   DASWBY010000582 DASWBY010000000
VERSION     DASWBY010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285728"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..404)
                     /locus_tag="VF870_10555"
     CDS             complement(30..404)
                     /locus_tag="VF870_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9252674.1"
                     /translation="MKSRSLFDSEDFQIDIVKGISIIKVNLPRTTFNKADEFKQLLDT
                     LLAAKHNKIIIDFSNCHYIDSMVVGVMVKSVKDARKKKGDILVITPSGSINVMFARTG
                     LYKIFKQFWSVEKAIESFSETN"
     gene            657..1106
                     /locus_tag="VF870_10560"
     CDS             657..1106
                     /locus_tag="VF870_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9252675.1"
                     /translation="MNKRNMAKHLASLTVKLVASYQENYTRIAMERGITESEFRCLRL
                     MGLEKGLSNKRIADRMSLSQSRLTRIVDGLCEKGFMTRESNPKDFRSLRLTLSRKAKS
                     LIQKMDKLNEDLHYKILNEIGIPQQKALITAMEKLYAEMPKWLEKFK"
     gene            1137..1886
                     /locus_tag="VF870_10565"
     CDS             1137..1886
                     /locus_tag="VF870_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX9252676.1"
                     /translation="MFKTFFYLLNILFFFIIDISAQDATAIVKKADELMRAKSSYTEM
                     TMKIVKPDWSREMSMKVWALEPDYAMIYITEPARDKGTVTLKRKNEVWNWLPTAQKVI
                     KIPPSMMLQSWMGSDFTNDDLVRESSIIKDYTHKLIGEEKVDGYDCYKIQLTPKPEAG
                     VVWGKIITWIAKDSYLQPKTEYYDEDGFIIKIFIGSNLQKMDGRNIFTHWEMIPEDKP
                     GNKTIMDYNKIQFNINTDESFYSEQNMKRVR"
     gene            1883..3103
                     /locus_tag="VF870_10570"
     CDS             1883..3103
                     /locus_tag="VF870_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9252677.1"
                     /translation="MNTYIKLAWRNLWRNKKRTLIAAASVFFAVILALLMRSMQTGYY
                     DYMIDASVRMYTGYIQIHGKDYWEKKSLDESMQLDQNQLNDLKNIPGVTLTVPRFETY
                     SLISHGNKTKVVQITGINPKLEDQLTDIKKKIVNGKFLDDSSKGIILAKGLAEILKVD
                     VGDSVVIYGQGYHGVTAAAQIPIKGIIEFTLPELNKSFTYLSLAYAQWLFSAPDRLTS
                     LSIMVDNPKELDRIKADIRNVFDEKYEVLDWEELSPELVQSIQVDNASGIIMLGILYI
                     VIAFGIFGTIMMMTAERVKEFGILISVGMKKWKLGLVSFFETLFISFIGVFSGALISI
                     PILLYFKANPIPLTCEYAKAITEWGFEPILPFAVYPGMFFAQIWTVLAIAMVSVIYPV
                     LFIRKLKPVEALRG"
BASE COUNT         1109 a          493 c          594 g          969 t
ORIGIN      
        1 taatcaaatg aatcaatatt taggtctgat caattagttt cagaaaaact ttctattgct
       61 ttttcgactg accaaaattg tttaaagatt ttatacaatc ccgtccgtgc gaacataaca
      121 ttaatactgc cggatggagt aatgacaaga atatcaccct tcttttttct agcatcttta
      181 acggacttga ccataacgcc aacaaccatt gaatcaatat agtggcagtt ggaaaaatca
      241 attataattt tattatgctt agctgctaat aaagtgtcaa ggagttgctt aaattcatcg
      301 gctttattaa aagtagttct tggcaaattt actttaataa ttgatatgcc ttttactatg
      361 tcaatttgaa aatcttcgga atcaaacagt gatcttgatt tcatgttcct gcacgaataa
      421 ttaataattt attttactaa cgacaaaata actattattt aatattatta cagcaatatt
      481 atcgttcctg tcttttttat attaaatata tacttcacta ttaagattca ttacaccaga
      541 atatcgattg ccaaatatta agcattcatt gattctttta aattaaatgt gcaaatttat
      601 tcaagaatta attaaatttt caaaaacaca tataaactat taatggaata attattatga
      661 ataaaagaaa catggcaaaa catctggcaa gcctgacagt aaaattagtt gcaagctatc
      721 aggaaaatta tacccgcatt gcaatggagc gaggaataac tgaatcagaa ttcagatgcc
      781 ttcgtctgat gggtcttgaa aaaggactta gcaacaaaag gattgctgat agaatgagtc
      841 taagccaaag ccgattaaca agaatagttg atggtctttg tgaaaaagga tttatgacaa
      901 gagagtcaaa tccaaaggat tttagaagcc ttagattgac tttatccagg aaagcaaaat
      961 ccttaatcca aaaaatggat aaacttaatg aagatcttca ttacaaaatt ttgaatgaga
     1021 tcggcattcc ccagcaaaaa gctttgatta cggcaatgga aaaactctat gctgaaatgc
     1081 cgaaatggct ggaaaaattt aaatagaaat ttttgttaga aacaaaaagg ttcgaaatgt
     1141 ttaaaacatt tttctatctg ctcaatattc ttttcttctt tattattgat atctctgcgc
     1201 aggatgcaac agcaattgta aaaaaagctg atgagctgat gcgtgctaaa tccagttaca
     1261 cagagatgac catgaaaatc gttaaacctg attggtcccg cgaaatgtct atgaaagtct
     1321 gggcacttga accagactac gcaatgatct acataactga accggcacgt gataaaggga
     1381 cagtaacgct taaaagaaaa aatgaggttt ggaactggtt gccgactgca cagaaggtaa
     1441 ttaaaattcc tccttcgatg atgctgcaaa gctggatggg atctgatttt acaaatgatg
     1501 atcttgtaag agaatcatct attataaaag attatacaca caagctaata ggggaggaaa
     1561 aagttgatgg atatgattgt tataaaattc agctgacacc aaaaccggaa gcaggagttg
     1621 tatggggtaa aattataacg tggattgcaa aggattctta cctgcagcca aagactgaat
     1681 attatgacga ggatggattt attataaaaa tatttatcgg cagcaacctg cagaaaatgg
     1741 atggcagaaa tatttttact cactgggaaa tgattcctga ggacaagccc ggcaataaaa
     1801 caattatgga ttacaataaa attcagttta acataaatac tgatgaatca ttctactccg
     1861 agcagaatat gaagagagtg agatgaacac ttatatcaaa cttgcatgga ggaatctgtg
     1921 gaggaataaa aaaagaactc tgatagctgc cgcatctgtt ttctttgccg taatacttgc
     1981 actgcttatg cgttctatgc agacaggtta ttatgattat atgattgatg cctctgttag
     2041 aatgtacaca ggttacattc agattcacgg taaagattac tgggaaaaaa aatctttgga
     2101 tgaaagcatg cagcttgatc agaatcaatt gaatgattta aaaaacatcc ccggagtaac
     2161 attaacagtt ccaagattcg aaacttacag ccttatttcc catggcaata aaacaaaggt
     2221 tgtacagata acaggtataa atcctaagct tgaggatcag cttacagata tcaaaaagaa
     2281 aattgttaat ggaaaatttc ttgatgattc ttcaaagggg attattcttg caaagggatt
     2341 ggctgaaatt cttaaagtcg atgtcggcga tagtgttgta atatatggac agggttatca
     2401 tggagttacg gcggcggctc aaattccaat aaaaggcatt attgaattca cattaccaga
     2461 gctgaataaa tccttcactt atcttagcct tgcttatgca caatggcttt tctcagcgcc
     2521 tgatagatta acttctctat cgataatggt agataatccc aaagagttag ataggattaa
     2581 ggcggatatc aggaatgtat ttgatgaaaa atatgaggtg ctggattggg aggaattatc
     2641 gccggaactg gtacaatcga ttcaggtgga taatgcttca ggaataataa tgcttggtat
     2701 tctttacatc gtaattgcat ttggaatatt cggaacaata atgatgatga ccgccgaaag
     2761 agtaaaagaa tttggaattc ttatttcggt tggaatgaaa aaatggaaac ttggtcttgt
     2821 atctttcttt gaaactttat tcatttcatt tattggagtt ttttctggag cactgatcag
     2881 tattccgata ttattatact ttaaagcaaa tccgattcca ttaacgtgcg aatatgcaaa
     2941 agcaattact gagtggggat ttgaaccaat tttaccattt gcagtttacc ccggaatgtt
     3001 ctttgcacaa atctggacag tgctggcaat tgcaatggtt tctgttattt atccggtctt
     3061 gttcattaga aaactaaaac cagtagaagc acttagaggc tgatatgtta ttaacactcg
     3121 cctggagaaa tatctggaga aataagaaac gcagtttgat tattg
//
LOCUS       DASWBY010000583         5286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_738848, whole genome shotgun sequence.
ACCESSION   DASWBY010000583 DASWBY010000000
VERSION     DASWBY010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738848"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VF870_10575"
     CDS             <1..550
                     /locus_tag="VF870_10575"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX9252678.1"
                     /translation="KWGAWYFAYAYAGYIRSALRSVLQHVKAYSPSDILQQVNLTVYG
                     DTKISEVFATLSIIIINNKTNELKYSGAGDIPIIYRNSENELLSIKSKGLLLGFSKIG
                     DYEDNVIQLKEGEQVYLTTDGITDSRAKTLESFGEHRLYEIIKNIKPDENPLEIIQKK
                     FDEFTGSEYDDDISLILISVKS"
     gene            577..4383
                     /locus_tag="VF870_10580"
     CDS             577..4383
                     /locus_tag="VF870_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9252679.1"
                     /translation="MCERIKFVLIFTLLITILISTAAQPQSYLITKYSTDNGLPDNRV
                     NDIAQDSLGRIWIAMTSGIAMYDGFEWTKFGEKEGVPEVEYIRIKVDEKGTIWFLPLA
                     AYKSSILSFKNNKWGKINFSKTIFNKLNGLNSIEVIYQDEQPKIFVGSFVRGFLQYFN
                     GNWEYYSTRNGLICDSVTSIVYNHGNIFISTYRGVSVFSAKKDFKKMFFEGTPSNSKI
                     LKLLNWNNPKTLNNEILILSDSWIGTFDGSRFEKIFDHPLLPFVSLIDLSSLNFTNLG
                     EILYGNSSIIHSYSLKNNSMNYLHIEDPESDRGATTIFKDYEENIWISSLRGIYKLSY
                     IPFENMTKENGLLESEVSAISEFNSGSKFFGHNYGFSTQTEDKIFQTNIYPTKSSLKI
                     SRILDSYHDKLNDVIYFTSLQKGVGKLSKNGKLKWIALNKIENYYSIFEAGKNKIIVS
                     TDMGYAQIINDGLVLLNNNFDAIVRNSAVFNDSITYLATTKGVKIFKDGKDIQLFFHK
                     DNPTNLFSLYYSDLYGLLIGSIKGLYKLQKDSLVKFNFNEYDITDPIYFIIQDSIKNI
                     WLGTNNGVLKWDGKILKRYNKSDGLAGNETNRAAGFVDSKGNVWIGTDEGVSMYTGNE
                     SDYENLPPKIMLLDFKDQRNTNYAANVAISLDPEENNITFQYRGLSFLDEKSNSYQIR
                     LKELSGKWANEFYTNTESARFNNLAAGVYVFSVRVKNSKGIWSKWQSSAVITIDKFYY
                     EQIPFQMGVFALFLFLTYSVYSYIQQKKYTQKLEQAVDLRTKNLRDTQVELITSIDRY
                     KGIVDSQADLVVRFDANGFFTFVNDAFCIVFGKSREELLGSSFTPFVHPEDLEKTREE
                     IEKLKVPHYRAIIELRILTSDGYRWFSWEGYAIHNSDGSIMEIQGVGRDITIQREIES
                     ELEKRVKERTVELESLISQSPMGIITFNSEGYILNYNNLANQLFGNLSSLIYPDKKFN
                     LFEDEFLLKNNYKDSLGNLDSSNGIIISKPIKIDDIKNEIYKNLVNHFLIYRIYTVTF
                     DENNKIYVLLLEDVTEQQKSEEINNRLLEEKIRFSTIIKTIETERDRIAKELHDGLGQ
                     MLTTAKLKLDIIKLKSDKNREEINDALGMLINAGDEIRRIINDLKPSDVERFGLVASI
                     ELLCERIKQTSKINVQFSVSNFNSLSDKKDELVLYRIVQEALNNIVKHANCNNAEINM
                     YRSNGLLNIEIWDDGSGYEMGKINDQLNKFGISNIKERVKNLNGQIDIRTQVGEGVQI
                     KIKIPV"
     gene            4390..5061
                     /locus_tag="VF870_10585"
     CDS             4390..5061
                     /locus_tag="VF870_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9252680.1"
                     /translation="MPEKIKILLVDDHPVLREGIKTVLELKGDFEIIGEADSGFRACE
                     LVPRLNPDVVLMDIRMEPMDGIKASRNIKALYPKTKILLLTMHDEADYILEALKIGVE
                     GYILKISDMEQVVNAIKLIMENETYFDPKITKSVASVSNDIVRKSKRNEILDEYCLTS
                     REIEIAGLMVDGLSTKQIADKLNISISTVSNHRRNIFIKLKVTKLSELISFAIHQGVF
                     IFKEE"
     gene            complement(5066..>5286)
                     /locus_tag="VF870_10590"
     CDS             complement(5066..>5286)
                     /locus_tag="VF870_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX9252681.1"
                     /translation="QSAIRKSMIGSGDRSDKIRTYNYPQNRITDHRIGLTLYNLSNIM
                     EGHCEELIEQLKIADKTEKLQSAETEQF"
BASE COUNT         1963 a          685 c          940 g         1698 t
ORIGIN      
        1 aaaatggggt gcctggtatt ttgcgtacgc gtacgcaggt tacatacgaa gtgcattaag
       61 aagcgtactg cagcatgtta aagcttattc gccttcagac atattacagc aggttaactt
      121 aacagtttat ggtgatacaa aaatctctga ggtatttgca actttatcaa tcataatcat
      181 taataataag accaatgaac ttaaatattc gggtgccggt gatattccga tcatatacag
      241 aaattctgaa aatgaattgt taagtataaa atcaaaggga ttactgcttg gcttttctaa
      301 gataggtgat tacgaggata acgttataca attaaaagaa ggcgaacaag tttatttgac
      361 aaccgatggc attacagatt ccagagcaaa aacattagaa agctttgggg aacacagact
      421 ttatgaaatt ataaaaaata ttaagcctga tgaaaatcct cttgaaatca ttcaaaagaa
      481 gttcgatgaa tttacaggtt ccgaatacga tgacgatata agtttaattt tgataagcgt
      541 aaaaagttaa acagaaactg caaagagtaa aattttatgt gtgaaagaat taaatttgtt
      601 ttaatcttca ctttactaat tacaattctg atatcaactg cagctcaacc acaaagttat
      661 ctaatcacta aatactcgac agataatgga ttacccgata atcgtgtaaa cgatattgct
      721 caggattcac tgggtagaat ctggattgca atgacttccg gaattgctat gtatgatggt
      781 tttgaatgga caaaatttgg ggaaaaagaa ggagtaccgg aagtagaata tattagaata
      841 aaagttgatg aaaaggggac aatatggttt ttacccttag ccgcatataa gtcttccatt
      901 ttatcattta aaaataataa gtggggaaaa ataaatttta gcaaaaccat ttttaataaa
      961 ttaaatggct taaattctat tgaagtaatt tatcaagatg agcagccgaa aatatttgtt
     1021 ggatcattcg ttagaggatt tttgcagtat tttaatggta actgggaata ttattcaaca
     1081 agaaatggtt taatttgtga ttctgtaacg agtattgttt ataatcatgg gaatatattt
     1141 atttctacct accggggagt ctccgttttt tctgcaaaaa aagattttaa aaaaatgttt
     1201 tttgaaggaa caccatctaa ttcaaaaatt ctaaaactat taaactggaa taatcccaaa
     1261 acattaaaca atgaaatttt aatattaagt gattcatgga ttggtacatt tgatggtagt
     1321 agatttgaga aaatatttga tcatccactc ttgccttttg taagtttaat cgatttatca
     1381 tctctaaatt ttactaattt gggtgaaata ttgtatggga attcttctat aattcattca
     1441 tatagtttga aaaacaactc aatgaattat ctacacattg aagatcctga aagtgacaga
     1501 ggggcaacaa caattttcaa agattacgag gagaacattt ggatttctag tttaagagga
     1561 atttacaaat tatcatacat tccatttgaa aatatgacta aagaaaacgg tctattagaa
     1621 tcggaagtta gtgctatttc tgaatttaat tccggatcca aattctttgg acataactat
     1681 ggtttttcaa ctcaaacaga agataaaata tttcaaacaa atatctatcc aacaaagagt
     1741 agtctaaaaa tttctcgtat tctcgattct tatcatgaca aacttaatga tgtaatttat
     1801 tttacctctt tgcaaaaagg agttggaaaa cttagtaaaa acggaaaatt gaaatggatt
     1861 gccctaaata aaattgagaa ttattattca atatttgaag ctggtaagaa taaaataata
     1921 gttagtacag atatgggtta cgctcagatc attaatgatg gtctagtatt attaaataat
     1981 aattttgacg caattgttag aaacagtgcc gtttttaatg attcaattac ttatttagca
     2041 acaactaaag gagtaaaaat atttaaagat ggaaaagata ttcagttatt ttttcataaa
     2101 gataacccca ctaatctatt ttccttgtat tactcggatt tatacggact tttaatcggt
     2161 tctataaaag gattgtacaa actccaaaaa gattcactag ttaaattcaa ctttaatgaa
     2221 tatgatatta cagaccccat ttattttatc attcaggatt ctataaaaaa tatttggctg
     2281 ggtacaaata atggagtttt gaagtgggat ggaaaaatcc ttaaacgtta taacaaaagt
     2341 gatggattag caggtaatga aacaaacaga gctgcaggat ttgttgatag taaaggaaat
     2401 gtctggattg gtactgatga aggagtgtcg atgtacactg gaaatgaatc cgattatgaa
     2461 aaccttcccc ccaaaataat gcttctggat tttaaggatc agagaaatac aaattatgca
     2521 gctaacgtag ccattagctt agatcccgag gaaaacaata ttacttttca atatcgaggg
     2581 ttgtcatttt tagatgaaaa aagtaattca tatcaaataa ggttaaaaga attaagcgga
     2641 aaatgggcaa atgaatttta tactaataca gaatctgcaa gattcaataa ccttgctgcc
     2701 ggtgtttacg ttttttcagt tcgcgtaaaa aactctaaag gtatttggag taaatggcaa
     2761 agttccgcag tgattacaat agataaattc tactatgagc aaattccttt tcagatgggt
     2821 gtgtttgctc tgtttttatt cttaacgtat agtgtataca gttatattca acaaaaaaag
     2881 tacactcaaa aattagagca agctgtagat ttaagaacca aaaatcttcg ggatacacaa
     2941 gttgaattaa ttacaagtat tgatagatat aaaggtatag ttgattcaca ggcagatcta
     3001 gtagttagat ttgatgcaaa tggtttcttt acttttgtta atgatgcttt ttgtatagtc
     3061 tttggtaagt cccgtgaaga attactagga agttcattta ctccatttgt tcatcctgaa
     3121 gatttagaga aaacaaggga agagatagag aaattaaaag ttcctcatta tcgtgcaata
     3181 attgaactac gtatattaac aagtgatgga tacaggtggt tttcctggga aggttatgca
     3241 attcacaata gcgatggaag cataatggaa atccagggag ttggaagaga tataacgatt
     3301 caaagagaaa ttgaatccga gttggaaaaa cgtgttaaag agagaacggt tgaattagag
     3361 tctctcatat cgcagtctcc aatgggaatt attactttta actcagaagg atatatactt
     3421 aattataata acctggctaa ccaattattt ggaaatttaa gtagcctcat ttatccggat
     3481 aaaaaattta atctatttga ggatgaattt cttctaaaaa ataattataa agacagtcta
     3541 ggcaatcttg attcatcaaa tggaataata atcagcaaac caataaaaat tgatgatatt
     3601 aaaaatgaga tttataaaaa tcttgttaac cactttctga tttatagaat ttatacggtt
     3661 acttttgatg aaaataataa gatttatgtg cttctattgg aagacgttac cgaacagcag
     3721 aaatccgaag aaataaataa tagattactg gaagaaaaaa ttagattctc tacgattatt
     3781 aaaacgatcg aaacggaacg ggatagaata gctaaagaat tacacgatgg cctgggacag
     3841 atgcttacaa ccgctaaatt aaaactagat atcataaagc ttaaatctga taaaaacaga
     3901 gaagaaataa atgatgcttt aggtatgcta ataaatgccg gtgatgaaat cagaagaatt
     3961 attaatgatt taaaaccatc ggatgtagaa agatttggac ttgttgccag tatagaatta
     4021 ttgtgtgaga gaattaaaca gacatctaaa attaatgttc agttttcagt ttcaaatttt
     4081 aattctctat ctgataagaa agatgaattg gttttatacc gaattgttca ggaagcattg
     4141 aataatatag ttaagcatgc caattgtaat aatgccgaaa ttaatatgta tagatcaaat
     4201 ggattactaa atattgaaat atgggatgat ggttcaggtt atgaaatggg aaagataaat
     4261 gatcagctaa acaagtttgg tatttccaac ataaaagaaa gagttaaaaa cttaaatggc
     4321 caaattgata tacgaacaca ggttggtgaa ggtgtgcaga taaaaattaa aattcctgtt
     4381 tgatttaata tgccggaaaa aataaaaata ttattagttg atgatcaccc ggttttgagg
     4441 gaaggaatta aaacggtact tgaactaaaa ggtgattttg aaattattgg agaggctgat
     4501 tcgggtttta gagcttgtga attagtccca agattaaatc ctgatgttgt tttgatggat
     4561 attaggatgg aaccgatgga tggaattaaa gcttcaagaa acattaaagc attatatcct
     4621 aaaacaaaaa ttttattatt aacgatgcac gatgaagccg attatattct cgaggcatta
     4681 aaaattggcg ttgaagggta cattctcaaa atatctgaca tggaacaagt agttaatgca
     4741 ataaaattaa taatggaaaa tgaaacctac tttgatccta agattacaaa atcagttgca
     4801 tcagtttcta atgatattgt acgaaaaagt aaacgaaatg aaattcttga tgaatattgt
     4861 cttacatctc gtgaaattga aattgcaggg ttaatggttg atggactctc aacaaaacaa
     4921 atagcggata aattaaatat tagtatttca actgtcagca atcatcgcag aaatattttt
     4981 ataaaattga aagtgacaaa actaagtgag ctaattagct tcgctattca ccagggtgtt
     5041 tttattttta aggaagaata aaaatttaaa attgctcagt ctcagcagat tgtagtttct
     5101 cagttttatc agcaatcttt aactgctcta tcaattcttc gcaatggcct tccattatat
     5161 ttgataggtt atataaagta agtccgattc tgtggtcggt aattctattt tgaggataat
     5221 tataagttct tattttatca cttctatcac cgcttccaat cattgatttt cttatagcgg
     5281 attgtt
//
LOCUS       DASWBY010000584         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_285814, whole genome shotgun sequence.
ACCESSION   DASWBY010000584 DASWBY010000000
VERSION     DASWBY010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285814"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1804
                     /locus_tag="VF870_10595"
     CDS             <1..>1804
                     /locus_tag="VF870_10595"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9252682.1"
                     /translation="LKNSNPDLYNQLADEEIKWAYDTWIKNHEPSDDNVLGVYDYRQY
                     SYQRYLFGFGGPLGADPNILKFYVSGEYKRNPTRLPTPEKVQTGQNYILNLTYQPGGG
                     HKFKIMGSYQSYIGGIWSGSSDIRWSGLAFTPPGVSTKYLVTIDPVREEQTIAQTLNW
                     VYSINPQSFTELTLTHQQEKYELPFKYLAGYGLEVDRADSSKDPSGTVLRDGSWWEKD
                     LFRAPFSFSTNYYQDSRTENFGFKFDYTGQIDVSHLAKAGIGIQYWDMINNGVNSSFQ
                     ANTYVARNGFADYYTAYPISIGGYVQDRMEYEGIIANIGVRAEFYNFQTQVPIDPFNY
                     LYVGTEGPGGTGNPATVGSETKLIFLPRFGISFPIGEGTAFRFQYGHFSSMPIFSQAL
                     SKKTWVGWQGLGNPNLDPKKTINYEFGLQQQLSEIHRLDLVLYYNDRVSQIGSQYLAA
                     YTGSKNVTQTPGGIYAGFDLENNPLYFINTYANNGFGSTLGFEATFETFSTKNWSYRL
                     SYSLSQTTAGNYGAQAQWPDNSREYSTRNFTGEFLASWDRTHNFRALLQYFVDVDGGF
                     EIFGIKPFSNSVFSLTYTAQSGLPYTYITSFDLKD"
BASE COUNT          583 a          295 c          361 g          565 t
ORIGIN      
        1 ttaaaaaata gtaatccaga tttatataat cagctagctg atgaagaaat aaaatgggca
       61 tatgatacct ggataaaaaa tcatgagcca agtgatgata atgtattggg agtttatgac
      121 tacagacaat attcttatca aagatattta tttggatttg gcggtcctct tggtgctgat
      181 ccaaatatct taaaatttta tgtttctgga gaatacaaaa gaaatccgac aagactacca
      241 acaccggaaa aagttcaaac aggacaaaac tatatactta acttaactta ccagcctggc
      301 ggcggacata aatttaaaat tatgggaagc taccaaagtt atattggcgg tatttggtca
      361 ggctcctctg atatacgatg gtccggtttg gcatttacac cccctggtgt ttctacaaaa
      421 tatctcgtaa ccattgatcc tgttagagaa gaacaaacaa ttgctcaaac attaaattgg
      481 gtttactcaa ttaatccgca atcatttaca gaactaactt taacacatca acaggaaaag
      541 tacgaacttc catttaagta tcttgccgga tacggacttg aggttgatcg agctgatagc
      601 tcaaaagatc caagcggtac tgtactaaga gacggaagct ggtgggaaaa agatttgttc
      661 agagcgccgt ttagtttcag cactaattat tatcaggata gcagaacaga aaactttgga
      721 tttaaattcg attataccgg acaaattgat gtatcgcatt tagcaaaagc aggtatcggc
      781 atccaatatt gggatatgat taataatggc gttaactcta gttttcaggc aaatacttat
      841 gttgctcgta atggttttgc agattattac acagcatatc caataagcat tggaggttat
      901 gttcaggata gaatggagta tgaaggtata attgctaata taggtgtgcg ggcagaattt
      961 tataattttc aaactcaggt tcctattgat ccttttaact atttgtatgt tggtacagaa
     1021 ggaccgggtg gaaccggtaa tccggctact gttggttctg aaacaaaatt aatatttctt
     1081 ccacgttttg gaatatcatt tccgattggt gaaggtactg ctttcagatt tcaatacggg
     1141 cacttttcat cgatgccgat ttttagtcag gcattatcta aaaaaacatg ggtaggctgg
     1201 caagggttag gtaatccaaa tcttgatcct aaaaaaacga ttaattatga atttggtttg
     1261 cagcagcagt taagtgaaat tcatagattg gatttggtcc tgtattataa tgaccgcgta
     1321 tctcagatag gaagccaata tcttgccgca tatacaggaa gtaaaaatgt aactcaaact
     1381 ccgggtggta tttatgcagg atttgatctc gaaaataacc cattgtattt cattaatact
     1441 tacgcaaata atggctttgg ttctacatta ggatttgaag caacttttga aacattctct
     1501 actaaaaatt ggtcgtatag attgtcctat agtctttcgc aaacaaccgc tggtaattat
     1561 ggtgcgcagg cccagtggcc tgataattca agagaatact caacaagaaa ttttacaggc
     1621 gaatttttag caagctggga tagaacacat aactttagag cgttgctgca atattttgtt
     1681 gatgttgatg gtggttttga aatttttgga attaaacctt ttagtaattc cgtctttagt
     1741 ttaacataca cagctcagtc agggttgcca tacacttata ttaccagttt tgatttaaag
     1801 gatg
//
LOCUS       DASWBY010000585         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_174229, whole genome shotgun sequence.
ACCESSION   DASWBY010000585 DASWBY010000000
VERSION     DASWBY010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174229"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..968
                     /locus_tag="VF870_10600"
     CDS             3..968
                     /locus_tag="VF870_10600"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX9252683.1"
                     /translation="MKKISKLDSASAIAIRDCMGAKKNEKILVITDGQKREIGLSLHE
                     NAIRLGYESLLVEMKSGKINGEEPSDVVADLMQKFDVVFCPTAKSLTHTDARRKASAK
                     GVRIATFPGITKEVMIRGMNADYKSISKRTIKLTNLLETGKEIRVTAAAGTDIMFSIA
                     GRKAYASKGLFHAKGESGNLPTGEAFLAPVEGTSNGVFVVDGSMAGLGLIKNANIKIE
                     VENGYATKISGGTLAKKLKVMLDKVGKDARNIAEFGIGTNDSAKLSGVLLEDEKVMGT
                     IHIALGNNVSMGGSVNVPIHLDGVVKKPTVWMDGKLLMKDGKLLV"
     gene            1115..1693
                     /gene="def"
                     /locus_tag="VF870_10605"
     CDS             1115..1693
                     /gene="def"
                     /locus_tag="VF870_10605"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9252684.1"
                     /translation="MKKEQMPILPITVCGDKILRKKAAKVSKVDNKIIKLIADMFETM
                     RNANGIGLAANQVGANKQIFVVDISMVEGYEDTKPLALINPKIVAKSEETIAYEEGCL
                     SIPDQHEEVIRPKKITIEFQDMDLAKHTIDADDLLARVMQHEYDHLQGILFTDLLDEE
                     TKKRLKKPLNKIKKRKIEVAYPISQSADYQIL"
     gene            1709..>2249
                     /locus_tag="VF870_10610"
     CDS             1709..>2249
                     /locus_tag="VF870_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX9252685.1"
                     /translation="MKIVFMGTPDFSIPSLKILIKNNHQVLAVVTTPDKERGRGQKIT
                     FTAVKQFAIENSIPVYQPEKLKGNLEFVEQMKNLNPDLFVIVAFRILPKEVFEIPKFG
                     SFNLHGSYLPKYRGAAPIQWALINGDTETGLTTFKLAEKVDTGNIYLQEKVAINPDDN
                     FETLHDRMSLLGADLVLKTV"
BASE COUNT          814 a          321 c          444 g          670 t
ORIGIN      
        1 aaatgaaaaa aataagtaaa ctcgattcag catcagctat tgcgattcgt gattgtatgg
       61 gtgctaagaa gaatgaaaaa attcttgtta taacagatgg acagaaacgc gaaattggat
      121 tatcattaca cgagaatgca ataagattag gatatgaatc tttactcgta gaaatgaaat
      181 caggaaaaat aaacggcgaa gaaccatcag atgttgttgc cgacttaatg cagaaatttg
      241 atgttgtatt ttgtccgact gcaaagtcac ttacacatac agatgcaaga agaaaagcct
      301 ctgccaaagg tgttcgcata gcaacttttc ctggaataac aaaagaagtt atgatccgtg
      361 ggatgaatgc cgattataaa tccatatcca aaagaactat taagctcact aacttattag
      421 aaacaggtaa agaaattaga gtaactgcag cagcgggaac tgatattatg ttttcaatcg
      481 ccggaagaaa agcttatgca agtaaaggtt tgtttcacgc aaaaggtgaa agcggtaatc
      541 tacctacagg cgaagcattt ttagctcctg ttgaaggcac aagcaatggt gtttttgttg
      601 ttgatggttc tatggctggt ttaggtttga ttaaaaatgc aaatattaaa atagaagttg
      661 aaaatggtta cgccacaaaa atatcaggtg gaactctcgc taaaaaatta aaggtaatgc
      721 tggataaagt tggaaaggat gcacgcaata ttgctgagtt tggaattggt acgaatgact
      781 ctgcaaaact tagcggagtt ttgcttgaag atgagaaagt tatgggtact attcacatag
      841 ctttgggaaa caatgtttct atgggcggaa gtgtaaacgt tccaattcat ttagatggtg
      901 ttgttaaaaa accaacagtc tggatggatg gaaaattatt aatgaaagat ggtaaactat
      961 tagtttaata ttaaatgtta agtgctcaat ttattatttt agtcatcgtt gatatctaaa
     1021 tcattaaaag attaggatta agtttttatc attatttaag tactttataa ttgcctgcct
     1081 attttttatt tttatactat aaataaatca agaagtgaaa aaggaacaga tgccaatact
     1141 gccaatcaca gtctgcggtg ataaaatatt aagaaaaaaa gctgcaaaag tttcaaaggt
     1201 tgataataaa ataatcaagc tgattgcaga tatgtttgaa actatgcgta atgcaaatgg
     1261 tatagggctt gcagccaatc aggttggtgc aaataagcag atctttgtag ttgatatttc
     1321 tatggttgaa ggttatgaag acacaaaacc attagcactt ataaatccca aaattgttgc
     1381 aaaatctgaa gagactattg cctatgaaga agggtgttta agtattccag atcaacatga
     1441 agaagttatc agacccaaaa aaataactat tgaatttcag gatatggatc ttgcaaagca
     1501 cacgattgat gccgatgatt tacttgcacg agttatgcag cacgaatatg accatctgca
     1561 aggaattctt tttacagacc ttcttgatga agaaactaaa aaaagattaa agaaacctct
     1621 gaataaaatc aaaaagagaa aaattgaagt tgcctatcca attagtcaat cagcggatta
     1681 ccagatttta taaattttaa ggtgagtgat gaaaattgtt tttatgggca ctcctgattt
     1741 ttcaatcccc tcattaaaaa ttcttattaa aaataatcat caagttcttg cagtagttac
     1801 tacccccgat aaagaaaggg gaaggggaca gaaaataaca tttacagcgg ttaaacaatt
     1861 tgcgatagaa aacagcattc ctgtttatca gccggaaaaa cttaaaggaa atcttgaatt
     1921 tgttgagcaa atgaaaaatc tgaatccgga tttatttgta attgttgcat ttagaatttt
     1981 acccaaagaa gtttttgaaa ttccaaagtt cggctcattt aatcttcacg gttcatatct
     2041 tccaaaatat cgaggcgctg cgccgataca atgggcattg attaatggag atactgaaac
     2101 aggtttaact acttttaaac ttgctgagaa agtagataca ggaaatattt atcttcaaga
     2161 gaaagtggcc ataaatcctg atgataattt tgagactctt cacgatcgaa tgagtttact
     2221 tggggcggat ttagttttaa agactgttg
//
LOCUS       DASWBY010000586         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_510647, whole genome shotgun sequence.
ACCESSION   DASWBY010000586 DASWBY010000000
VERSION     DASWBY010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510647"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1636
                     /gene="hutH"
                     /locus_tag="VF870_10615"
     CDS             71..1636
                     /gene="hutH"
                     /locus_tag="VF870_10615"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX9252686.1"
                     /translation="MARKKLIVDGKSLSLDKIEFFLKENPIVSLSNESKKNVIKARKL
                     IDKWVDEGKVIYGVTTGFGEFANVSISKKDIEKLQENLIVSHSTGVGDPLPPFIVKIM
                     MLLRVNALASGHSGIRLETLELLIAMINNNIIPVIPSQGSVGSSGDLAPLSHLVLAMI
                     GKGEAQIYNDVMKDDQHKVKVLNSSVALKKFGLTPVKLAAKEGLALINGTQMMTAFAS
                     YICIEAKKLEKIADIAGALSHETLRGTDNAFDLRLHELRPFPGQITVAKNILAMIKGS
                     EIRESHRENDARVQDSYSIRCIPQIHGASRDSIDYVCSRVEIELNSVNDNPLIFPEAE
                     AHLEGGNFHGQPIALAMDFMAIALSEYANVSERRTERMLNGSLSSLPRFLAKNGGLNS
                     GLMISQYTAAALVSENKVLAHPASVDSIPTSANQEDHNSMGSIAARKCFQILKNVQAV
                     LAIEILTACQGLEFHKPMKPGLGNRIAYELIREKIPAQENDRIIYKDIESVKTILTKN
                     VLINEVTKKIKLQ"
     gene            1653..1958
                     /locus_tag="VF870_10620"
     CDS             1653..1958
                     /locus_tag="VF870_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252687.1"
                     /translation="MNKTFLLTFEHGPNYQPDMIFHDQPDWENHSRFADKLFLQHKVT
                     MCGALKDESKVIVVALGKNESSLRNLFKDDPFVKNGVLTLTHVDAWELHLNPEHVEE"
BASE COUNT          713 a          345 c          398 g          617 t
ORIGIN      
        1 gatgaagaca aaattaaaat tcattacaag acaagtaaac aaaattcaga taaaattaaa
       61 aagataattc atggctcgta aaaaattaat agtcgatggt aaatcactat cgttagataa
      121 aatagaattt ttcttaaaag aaaatccaat tgtttcttta tccaatgaat caaaaaaaaa
      181 tgtaatcaaa gcacgcaagc taattgataa atgggttgat gagggaaagg taatttacgg
      241 agttacaact gggttcggtg agttcgcaaa tgtttcaata tctaaaaaag atattgagaa
      301 actccaggaa aatttgatag tctcccactc aacaggagtt ggtgatccgc taccgccgtt
      361 tattgttaag ataatgatgc ttcttcgtgt taatgcactt gcaagtggtc attctggaat
      421 tcgacttgaa acactcgaac ttttaattgc aatgattaac aacaatatca tcccggttat
      481 tccatcgcag ggttctgttg gttcaagcgg cgatcttgcg cctctttctc atcttgttct
      541 tgcaatgatt ggcaaaggcg aggctcagat ttataatgat gtgatgaaag atgatcagca
      601 taaagttaaa gttttgaatt catctgtggc gctaaagaaa tttggattaa ctcccgtaaa
      661 gcttgctgct aaagaaggct tagccttaat taacggtact caaatgatga ccgcctttgc
      721 atcgtacatt tgtattgaag caaagaaact tgaaaagatt gcagacattg caggagcatt
      781 atctcacgaa actttacgcg gaaccgataa tgcttttgat ttaagattgc atgagttgag
      841 accatttccc ggacaaatca ctgttgcaaa aaatattctt gcaatgatta aaggaagtga
      901 aatccgggaa tcgcacagag aaaatgatgc gcgtgttcag gattcatatt ccatacgctg
      961 tattcctcaa attcatggag cttcaagaga ttcgattgat tatgtatgtt cgcgggttga
     1021 gattgaactt aattctgtaa atgataatcc attaatcttt cccgaagctg aagctcatct
     1081 tgaaggcggg aattttcatg gtcagccaat agcacttgca atggatttta tggcaattgc
     1141 tctttcagaa tatgcaaacg tttccgaacg cagaacagaa agaatgttaa acggttcgct
     1201 tagcagttta ccaagatttc ttgcaaagaa cggcggatta aattcaggtt tgatgatttc
     1261 acaatacact gcagccgctc tcgtttcaga gaataaagtt ttagctcatc cggcaagtgt
     1321 tgattcaatt ccaacatctg caaaccagga agatcataat tcaatgggat caatcgctgc
     1381 aagaaaatgt tttcagatat taaaaaatgt ccaagcagtt ttagcaatag aaattcttac
     1441 agcctgccag ggattggaat tccataaacc gatgaagcca ggtttaggaa ataggattgc
     1501 atacgagtta atccgtgaaa aaattcctgc gcaggaaaat gataggatta tttataaaga
     1561 cattgaaagt gttaaaacaa ttctgacaaa aaatgttcta attaatgaag taactaaaaa
     1621 aataaaactt caataatctg aggaaattaa atatgaacaa aacctttcta ctaacattcg
     1681 agcacggacc gaactatcag cctgatatga tttttcacga tcaacctgat tgggaaaatc
     1741 attctcgctt tgctgataaa ctttttcttc agcacaaagt tacaatgtgc ggtgctctaa
     1801 aggatgaatc aaaagttatt gttgttgctt taggaaaaaa tgaaagctca ctaagaaatt
     1861 tgttcaaaga tgatccattt gtaaagaatg gtgttttaac actcacacat gttgatgcat
     1921 gggaacttca cttaaatcct gaacacgttg aagaataata tatgtatgtc tttgcgaatc
     1981 cggttttagt caggtgaagc aatctgttat gtcaaatagc gtgtaaaaat atttgcaatc
     2041 ttgtaaaatt tgagaatact tcatcgagga ctc
//
LOCUS       DASWBY010000587        12011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_478104, whole genome shotgun sequence.
ACCESSION   DASWBY010000587 DASWBY010000000
VERSION     DASWBY010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12011
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478104"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF870_10625"
     CDS             <1..663
                     /locus_tag="VF870_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9252688.1"
                     /translation="ALQRDLRLKSLPRKIECFDISNLQGTDTVASMVVFEDGKPKKSL
                     YRKFIIKTVEGPDDFSSMREVIERRYSRLLEEHQPLPDLIMVDGGKGQLSSAVEILDN
                     LGFRDYNIIGLAKRLEEVFLPNNSDPEIIPKTSSGLKLLQQVRDEAHRFAITFHRSRR
                     SKRTIKSELTDIKGIGTSIAKKLLETFGSFDSVKKSSYENLIKVVGKKKAEILLDYFS
                     KE"
     gene            726..1901
                     /locus_tag="VF870_10630"
     CDS             726..1901
                     /locus_tag="VF870_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252689.1"
                     /translation="MKKKITVFFLAFIIIGLSACKEKVEEKPFVFDGESFYTLVENSI
                     SGDENSKKILEGLFTFDADLKTFNKIEVDSIELNNENYYTLLLENQNPVYNLFAIVDK
                     DLNLVLKDESLNGYINLNFKKSGSRRFAVITEEFNSKDVIHLNRMSYYSLEQYSTELV
                     FRQFTKIKTPNKEAEQVIALISDTAIVTNILFPASKKGKTSKDIFRFDVSSNKYVSDK
                     NLFDSLIYKEIRSLNIQTKHLQITDAESILRFFKAKDSNISSNEINISENDFEIVLDS
                     TWKNLGSITITNLLKRKLKGIKFISGKLGAGISLAVIAPTDSAEIYFTEELKNRNGLN
                     SSMRYSDKLVDSKNIYQLYEFSCPSKKILMILETPKSTYEINKSQYEQIIKSFLIKC"
     gene            1901..2200
                     /locus_tag="VF870_10635"
     CDS             1901..2200
                     /locus_tag="VF870_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252690.1"
                     /translation="MYKQLYFLHKSENDKALPHFKDVILSHLSEISGKRIKIARVESN
                     LLLDQKYSYFCEVEFESKDKMNELMNSKAGKQLTKDLMDFHQMITMISINYDIEE"
     gene            2197..2676
                     /locus_tag="VF870_10640"
     CDS             2197..2676
                     /locus_tag="VF870_10640"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX9252691.1"
                     /translation="MNLYLIRHSIAENISIDKKDFDRELTEEGKFVINKASEAWKNYI
                     GNVDVVLTSPLIRAIQTAEIISSNLQNSPNVVKDNNLGTGSKTADLIELLNSLKYKNV
                     AVVGHQPDLSYHLNNFCGTGSFNIAFPPASLAKIEFENSIKYGRGKLIYFIPPTIYI"
     gene            complement(2800..2894)
                     /gene="ffs"
                     /locus_tag="VF870_10645"
     ncRNA           complement(2800..2894)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF870_10645"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2962..3915)
                     /locus_tag="VF870_10650"
     CDS             complement(2962..3915)
                     /locus_tag="VF870_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX9252692.1"
                     /translation="MSLSNKKRAPLNRTINISNAEIKSFSEKLLRLNRKTNLDNISGR
                     IINQDLFDVIEFLPNNFVDLLFLDPPYNLNKNFSSLNFREMKSDDYEIWLDSWISKVV
                     RILKPNASIYFCCDWKTSIPAYNILKKYFIIRNRITWEREKGRGSKDNWKNSYEDIWY
                     CTLSGKFTFNVDAVKLKRMVRAPYKTEAGAPKDWQSENGKNFRITHPSNLWTDISIPF
                     WSMPENTDHPTQKPEKLLAKLILASTNENDLVFDPFLGSGTTAVTAKKLNRKFCGVEI
                     DETFASLALKRLDIADKEKSIQGYEGGVFWERNSLQEQKKK"
     gene            complement(3933..7262)
                     /locus_tag="VF870_10655"
     CDS             complement(3933..7262)
                     /locus_tag="VF870_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9252693.1"
                     /translation="MTTENNSDQYSFVHQLESLLASSLDVIFRISPTGKFHFISQSSY
                     HLLGYDPEEILGRSIAEFIQPEKINYYFKEISILFRESNLNSFRMDLKHKNGSYIPVE
                     ITGKVVEIEGKLMGQGIIRDISSRIETQQKLASSENIFKAIWENSKDGMRLTDQDGII
                     VLCNNAFAELFGKSIGELINRPISDLYFGEKEEVSLANYKQNFIRGMVNDYVESSAIL
                     WNGKKLLFEISNSMIEASDDKRFVLSIFRDISDRKADEELLEKKGKLLQGIAKATNAL
                     ITDSAYFDGFNVALELLGEAAEADRVYIYKHVEDEETGELYFTPLFEWTAEGVYSQLE
                     ENLFGKLSYSRFASLKFYEAFSNGETLKFIIKNLPKEYQDVFIDRNIRSIILVPILIE
                     GAYWGFIGFDECHSDRIWSNDEESILIAMAATIGEVLRKNKFREELIRKNAELDIAVR
                     EAEKAAKARSEFLALMSHEIRTPMNGVIGMTGLLLESMLSESQKDYVNTIRLSGEQLL
                     VIINDILDFSKIESEKLELEVQPFDLRECIEDSMDLISTKVAEQDIELFYNIDENTPY
                     AIKGDITRLRQILTNLLSNAVKFTHKGEVEISVTSEHLDFKEYLFKFNVRDTGLGIPK
                     DKLDKLFKPFSQVDSSITRTFGGTGLGLVISKKLVELMGGEIGFTSVEGKGSDFYFTI
                     KAESVTSDKKVYLFESSTRLLGKRVLIIDHNKSNGMMLNNLAKRWRMSSDLISDLSEL
                     KMKLEINNDFDLFIINTSGYNNVRSLMDEIKNSISKKNIGFILIKSQVRDLGLTESDR
                     NPYCRILTKPVRRKQLHQAMLSFVEIGLNITQISSAEKEIKVFPAADKIKVLLVEDNN
                     VNQMVALRMLQRIGISADVASNGKEAVDAVKSIGYDLIFMDISMPEMDGLTACSIIKS
                     DSSLQKNPIIIAMTANAMSGDKENYLKVGMDDYISKPVNLEELSKIIFRWTEKIVSDK
                     HDETRKAIESEIELKFIEEKKISFLQDLKTDADLEFFKEMLDIYVREIPKNIEAIRNA
                     IFQNNSDHLRFYVHKLKGSSLTLGIECVLENFKILEDMALDNNISEESLRIFKKVSEQ
                     FEVILEEIVLLKNKYSHIKFP"
     gene            7429..8610
                     /locus_tag="VF870_10660"
     CDS             7429..8610
                     /locus_tag="VF870_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX9252694.1"
                     /translation="MKRTIVAMPGDGIGKTVLPEAIRVLNAVGFDAEYVHGDIGWDFW
                     CSEGNALPQRTVDLLEKYKIGLFGAITSKPKDAADKELDPSLKGKGFSYFSPIVGLRQ
                     KFNLDICIRPCQSFKGNPLNFIIRNAQGGFDEPLVNAVIFRQNTEGLYGGVEWTNPPK
                     QVRDALETHPKMKFFKDVSSEDMAISTRIVTRNASRRIIRAGFEYAKKYGYTNLTLCE
                     KPNVLRETSGMMFKVAKEIAKEYPGIALWDTNIDAQMMWLTKNPETYGVIVAENMFGD
                     IISDGFAGLVGGLGFACSANIGEEVAVFEPTHGSAPKYQELNPSIVNPIAMMLSACMM
                     LDHIDEKPKADKIRSAIAKVVEEGKVKTYDMMKLRGGPNVFSKGACTTQQMTDEIIKN
                     L"
     gene            8702..10336
                     /locus_tag="VF870_10665"
     CDS             8702..10336
                     /locus_tag="VF870_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEX9252695.1"
                     /translation="MNGIELVIIALVLYVAAYKLYGGFLSRRLEVNNKVETPAHTMSD
                     GVDYCPAKAPVLLGHHFASIAGAGPIVGPVIAAGFGWMPVYLWVVFGAIFIGGVHDYA
                     SIIASVRHQSKSIGYIIQSYIGISGKKLFLIFAWATLILVIAVFTIIVADTFTHIPSA
                     GTSSILFMFLAVLFGIAIYRLKIRLWIATMIGVILLFLSIPAGNLFPIQLSALTWQLI
                     LFGYIFIASTVPVWILLQPRDYLNSFFLYALMAGGLIGVFFAAPSINIPAFSTFSLDK
                     VGYLFPALFVTVACGAISGFHSIVGSGTTAKQLNKETDGRIIGYGGMLIEGMLAVLSL
                     IAVASMVNKEFIDILVTKGPVPAFSLGVARFINAIPVLNISIPMAQTFTALAVSAFAL
                     TSLDTATRLARFMFQEYFENKESEKKFVLVTNRYVSTAITVVFGAALTFSGQTMSIWP
                     VFGSANQLLAALALLALTVWVANLKKGYLFVLIPMIFMFAVTLTALGMLIYTNFIATN
                     YTLSIISILLFTLAVLLGLKAYSVLTNGKELSNEPA"
     gene            10349..10882
                     /locus_tag="VF870_10670"
     CDS             10349..10882
                     /locus_tag="VF870_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9252696.1"
                     /translation="MKEKVLKIWPEINWIENQALREKVLNCWVYAIENSVLSPEDLEV
                     IPFSLLIKDCKISFMNHKRTAVQLSVDIAKRMKANFKEEIKIDMDLLIAGAILIDIGK
                     LIEYDKVDGKLVTSKAGTLLRHPFSGVSIADRFGLPYEVQHIIAYHAKEGDLAKRNVE
                     AIIVHHADFVSFDPFKA"
     gene            10891..>12011
                     /gene="lysF"
                     /locus_tag="VF870_10675"
     CDS             10891..>12011
                     /gene="lysF"
                     /locus_tag="VF870_10675"
                     /EC_number="4.2.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitase"
                     /protein_id="HEX9252697.1"
                     /translation="MAQTLIGKIAQKFAVGLDPKHKVQAGDYLSIKPAYVMTHDNTGA
                     VIPKFKSIGATKLFNPRQVVNTLDHNVQDKTEKNLEKYKKIENFAKSMGVDFYPAGRG
                     IGHQIMCEEGYAWPGTMVVASDSHSNMYGGLGCLGTPIVRTDAAAIWATGRTWWQVPP
                     IAKVELKGSLRQGVTGKDVIIALCGYFNHDEVLNHAIEFVGDGIRYLTIDERLAIANM
                     TTEWGALAGVFPIDLATILWLKKRERFIEHRGLEGVFSDTDGKGEHPRINHKRIEELE
                     HNIPQADADAFYSKELIIDLSTIEPFVSGPNTVKTYAAVSELKEKNISINKAYLVSCV
                     NSRVDDIAEAAAVVKGKKVAEHVKFYIAAASSEVQKEAER"
BASE COUNT         3944 a         2033 c         2056 g         3978 t
ORIGIN      
        1 gcattacaaa gagacttaag attaaagtca cttccaagaa aaattgaatg ttttgatatt
       61 tctaacttgc aaggcacaga cactgttgca agtatggttg tgtttgagga tggaaagcca
      121 aagaaaagtt tatacagaaa atttattatt aaaacggttg aaggtcccga tgacttttct
      181 agtatgcgtg aagtaattga aagacgatac tcaagattgc tcgaagaaca tcagccattg
      241 ccggatttga taatggtcga tggtgggaaa ggacaacttt caagcgctgt agagatttta
      301 gataatcttg gttttaggga ttacaacata attggcttag caaaaagatt ggaagaggta
      361 tttttaccaa ataattccga tcctgaaata attccaaaaa catcatcggg attaaagtta
      421 ttacagcagg tccgtgatga agcgcatcgt tttgcaatta ccttccatcg ttctagaaga
      481 agtaaacgaa ccattaaatc tgaactaact gacatcaaag gaattggaac atcgatcgct
      541 aaaaaactac ttgagacttt tggcagcttt gattccgtta agaaatcttc atatgaaaat
      601 ttaattaaag ttgttggcaa aaagaaagct gaaatattgc tcgattattt tagtaaagaa
      661 taaattaata aatattaatt gaaagaatca ttttgcttat ctttgcttaa agaaatcaga
      721 tgaaaatgaa aaaaaaaata acagtatttt ttttagcttt tataattatc ggcctatctg
      781 cttgtaaaga aaaagttgaa gaaaaacctt tcgtttttga tggagaaagt ttttacaccc
      841 tcgttgaaaa ttcaatcagc ggtgatgaaa acagtaaaaa gattcttgaa ggacttttta
      901 cctttgatgc ggatttaaaa acttttaaca aaattgaagt tgacagcatc gaattaaata
      961 atgaaaatta ttatacactt ctgcttgaaa atcaaaatcc agtttataat ttgtttgcga
     1021 ttgtggacaa ggatttgaac ctagttctta aagacgaatc attaaacggt tatattaatt
     1081 taaattttaa aaaatcaggc tctagaagat ttgcagttat taccgaagag tttaattcca
     1141 aagatgttat tcaccttaac agaatgtcat actattcact tgaacagtac tcaacagaac
     1201 ttgtattcag gcaattcaca aaaattaaaa ctccaaataa agaagccgaa caagttattg
     1261 ctctaatttc tgatacagca attgtgacta atattctttt cccagcatca aaaaaaggta
     1321 aaacttccaa agatattttt agatttgatg tatcgagcaa taaatatgtc agcgataaaa
     1381 acttgtttga tagtctgatt tataaagaga tcagaagcct caatatccaa actaagcatc
     1441 ttcaaataac tgatgctgaa tctattctta gattttttaa ggccaaagat tctaatattt
     1501 cctcaaatga gattaacata agtgagaatg attttgaaat tgttcttgac agtacctgga
     1561 aaaatcttgg aagtattact ataacaaatt tactgaagcg aaaacttaaa gggattaaat
     1621 tcataagcgg taaactgggt gcaggaattt cgcttgcagt aattgctcca acagattctg
     1681 ccgaaattta tttcaccgaa gaattaaaga atcgtaatgg gttaaacagt tcaatgcggt
     1741 attcggataa acttgttgac agcaaaaata tttatcagtt atatgagttc tcctgcccat
     1801 caaaaaaaat tctgatgatt cttgaaactc caaaatcgac ttatgaaata aataaaagcc
     1861 aatacgaaca aatcataaaa tcatttttaa tcaagtgctg atgtacaaac aattatattt
     1921 ccttcataaa tccgaaaacg acaaagctct acctcacttt aaagatgtga tcttatctca
     1981 tctttctgag atatctggta agagaattaa aattgcgcgc gtcgaaagca atttattact
     2041 cgatcaaaaa tactcttact tctgtgaggt tgaatttgaa tctaaagata aaatgaatga
     2101 gttaatgaac tcaaaagctg gtaaacagct tacaaaagat ttgatggatt ttcatcaaat
     2161 gataacaatg atttcaatta actatgatat tgaagaatga atttatactt aataagacat
     2221 agcatcgcag aaaatatttc tatcgataaa aaagattttg atcgtgaact tactgaggaa
     2281 ggtaagtttg taataaataa agcttctgaa gcgtggaaaa attatattgg taatgttgat
     2341 gttgttttaa cttctccact catacgcgcg atccaaactg cagaaataat ttcatcaaac
     2401 cttcaaaata gtcctaacgt agttaaagat aataatcttg gtaccggcag taaaactgca
     2461 gatctaattg aacttcttaa ttcactcaaa tataaaaacg ttgcggtagt tggtcaccag
     2521 cctgatttat catatcatct aaataacttt tgcggaaccg gaagttttaa tattgccttt
     2581 cctcctgctt cgctggctaa aattgaattt gagaacagca ttaaatatgg cagaggaaaa
     2641 ttaatttact tcatccctcc aactatttat atataaggta attatttttc atttgtcatt
     2701 tcgaccgaag ggagaaatct gaaagataaa actaatcaga tttctcagtc tcccgacaag
     2761 tcgggatccc tcgaaatgac aataaaattg tattacaaca aaaaggggca cacagttaca
     2821 caaattatat actgaccgct gcttccttcc ggacctgacg gggttgggta ttaacctgtt
     2881 aaccggtgcc ctaaactcta aatcttgggc tgttaaaatg taaaattata tcttatcaaa
     2941 caagatttaa tttattgatt actatttctt cttttgctcc tgcaaactat ttctctccca
     3001 aaaaacacca ccttcatagc cttgaataga tttttcttta tcggcaatat caagtctttt
     3061 tagagcaagt gaagcaaaag tctcatcaat ttcaactccg caaaatttac gatttagttt
     3121 ttttgcagtt acagctgttg tacctgaacc aagaaatgga tcaaaaacta aatcattttc
     3181 attcgtactt gcaagaataa gttttgcaag aagtttttcc ggtttttgcg taggatgatc
     3241 tgtgttttca ggcatggacc agaatggaat tgaaatgtca gtccataaat ttgatggatg
     3301 tgtaattcta aaattctttc cattttcaga ttgccaatcc tttggtgctc ctgcttcagt
     3361 tttatatggt gctctaacca ttcgttttag ttttacggca tcaacattaa atgtaaactt
     3421 tccagataag gtacagtacc agatatcttc ataagaattt ttccaattat cttttgagcc
     3481 tctccccttt tcgcgctccc aggtaattct gtttcttatg ataaaatatt ttttcaagat
     3541 gttgtaagca ggaattgagg ttttccaatc gcagcaaaaa tagattgaag catttggttt
     3601 taatattcta actactttag aaatccagct atccagccag atttcataat catcactctt
     3661 catttctcta aagtttaggg aagaaaaatt tttattaaga ttatacggcg gatcaagaaa
     3721 caataaatca acaaagttgt taggcaaaaa ttcaatcaca tcaaacaaat cctgattgat
     3781 aattctaccg ctgatattgt ctaaattagt tttacgattt agtcttaata atttttcaga
     3841 aaaagattta atctcagcat tgctgatatt gatcgtgcgg tttaaaggag ctcttttctt
     3901 atttgaaagg gacattagat ttgattttta gattacggaa attttatatg cgaatattta
     3961 ttctttagca gaactatttc ttcaagtatt acctcaaact gttctgaaac ttttttaaat
     4021 attcgtaaag attcttcact tatattatta tcaagagcca tatcttcaag tatcttaaaa
     4081 ttttccagaa cgcattctat tccaagagta agtgaggagc cttttaattt gtgtacataa
     4141 aatctaagat gatctgaatt gttctggaaa atagcgttgc gaatagcttc aatgttttta
     4201 ggaatttccc ttacgtaaat atccagcatt tccttgaaga actccagatc agcgtcagtc
     4261 ttaagatcct gcaaaaaaga aatcttcttt tcttcaataa actttaattc tatttcagat
     4321 tcaattgctt ttctggtttc atcatgctta tcactgacaa ttttttctgt ccatctaaaa
     4381 attatttttg aaagttcttc taagttaacg ggcttgctga tgtaatcatc catcccaact
     4441 ttaagataat tttctttatc accggacata gcatttgccg tcatggcaat aatgatagga
     4501 tttttttgaa gtgaagaatc agattttata attgagcatg cagtaagtcc atccatttcc
     4561 ggcattgaga tatccataaa aatcaaatcg taacctatgg attttactgc atccacagct
     4621 tcttttccat tagatgcaac atctgcactg ataccaattc tctgcagcat acgcagcgca
     4681 accatttgat taacattgtt atcctcaacc aaaagaactt taattttatc agcagccgga
     4741 aatactttta tctctttttc tgcagaagaa atctgtgtga tgtttaatcc aatttcaaca
     4801 aaacttagca tagcttgatg aagttgtttt cttctaacag gtttggtaag tattcggcaa
     4861 taaggattac gatcagattc agtaaggcct aaatctctaa cctgagattt tataagtata
     4921 aaaccaatat ttttctttga aattgaattt ttaatttcat ccatcagtga gcgaacatta
     4981 ttatatccag atgtgtttat aatgaaaaga tcaaaatcat tattaatctc taatttcatt
     5041 ttaagttcag aaagatctga aatcaaatca gaactcattc gccatctttt agcaagatta
     5101 ttcaacatca taccattcga tttattatga tcgatgatca atactctttt gccgagaagt
     5161 ctggtcgaag attcaaacaa ataaactttt ttatctgatg taaccgattc ggcttttatc
     5221 gtaaagtaaa agtcgcttcc ttttccttca acactggtaa atccaatctc accacccatc
     5281 agttcaacaa gcttcttgct gatcactaat cccaaaccag tgcctccaaa agttcttgta
     5341 atagaggaat caacctggct gaatggtttg aaaagtttat caagtttatc ttttggaatt
     5401 cctaaaccgg tgtctctgac attaaatttg aaaagatatt ctttaaaatc taaatgttcc
     5461 gaggtaacac ttatctcaac ctcaccttta tgtgtgaact ttacagcatt acttaacaga
     5521 tttgtaagaa tttgtcttag tcttgtaata tcacctttaa ttgcatatgg agtattttca
     5581 tcaatattat aaaataattc aatatcctgt tcagcaactt ttgtagatat tagatccatc
     5641 gaatcttcaa tacattctct caaatcaaaa ggctgtactt caagttccag tttttcagat
     5701 tcaatttttg aaaaatcaag aatatcattt atgatgacaa gaagttgttc accgcttaac
     5761 ctgatagtat taacataatc tttttgagat tcactcaaca ttgattcaag caaaagacct
     5821 gtcataccaa ttacaccatt cataggcgtt cggatttcgt ggctcatcag agctaaaaac
     5881 tcacttctgg ctttagcagc tttttcagct tctcttacgg caatatctaa ttcagcattt
     5941 tttctgatta actcttcccg gaatttattt ttcctaagca cttctccaat tgtagcagcc
     6001 attgcaatta aaatagattc ttcatcattg ctccaaattc tatctgaatg gcattcatca
     6061 aatcctataa atccccaata agcgccttca atcagtattg gaaccaaaat tattgatctg
     6121 atatttctat caataaaaac atcctggtat tcctttggaa gatttttaat tataaatttt
     6181 aatgtttcac catttgaaaa agcctcataa aactttaaag atgcaaatcg agaataagac
     6241 aattttccaa ataaattttc ttctaattgt gagtaaactc cttctgcggt ccattcaaaa
     6301 agtggtgtaa aatacaactc gcctgtttcc tcatcttcca catgtttata aatataaaca
     6361 cgatcagctt cagcagcttc acccagtaat tcaagagcta cattgaagcc gtcaaaatat
     6421 gctgaatctg taattagtgc atttgtggct tttgctatcc cttgtaaaag ctttcccttc
     6481 ttttcaagca actcctcatc tgcttttcta tcgctgatat ccctgaaaat gcttagtaca
     6541 aatcttttat catctgaagc ttcaatcatc gaattagaaa tttcaaaaag taactttttg
     6601 ccattccaga gtatagcact tgattcaaca taatcgttaa ccataccacg aataaaattt
     6661 tgtttgtaat ttgcaagtga gacttcttcc ttttcaccaa aatacaaatc actaatcggt
     6721 ctgttaatta gttcaccaat acttttccca aataattctg caaaggcatt gttacaaaga
     6781 actataattc catcctgatc agtaagtctc attccatctt ttgaattttc ccagatggct
     6841 ttaaagatat tttcggaact ggctagtttt tgttgtgttt caattctact acttatatcc
     6901 cttattatac cctgccccat taattttccc tcaatttcaa ctacttttcc tgttatttca
     6961 accggtatat acgagccatt tttatgcttt aaatccattc tgaaagagtt gagattactt
     7021 tctctaaaga gaatcgatat ctccttgaag taataattaa ttttttcggg ttggataaat
     7081 tctgctattg atcttccaag aatttcttct ggatcgtatc caagtaaatg gtacgatgac
     7141 tgactaataa aatgaaattt accggttggt gagattctaa atattacgtc caaagatgaa
     7201 gcaagtaatg attcaagctg atggacaaat gaatattgat cagaattatt ttcggttgtc
     7261 aattgcttct tagtttaaat tcttttcgat aatcaattta atatttttac ttattaaaat
     7321 taaattttag agaaagaatc gtaaaagtaa gttcattttc cttgcttaca cattggcaaa
     7381 tatttaagtt tcgcctgtca ataatttcta attttacagg atttttttat gaagagaaca
     7441 atagttgcaa tgcccggaga tggcattggt aaaacagtac ttccggaagc aatacgggtt
     7501 ttaaatgcag tcggttttga tgccgaatac gttcacgggg atataggctg ggatttttgg
     7561 tgtagcgaag gtaatgctct tcctcagcga acagtcgatc ttcttgagaa atataaaatt
     7621 ggtttgtttg gagcgatcac ttcaaaacca aaagatgccg cagataagga acttgatccg
     7681 tcacttaaag gaaaaggatt ctcttacttt tcacctatag ttggattaag acaaaagttt
     7741 aatcttgata tttgtattcg tccctgccaa tcatttaaag gtaatccgtt aaattttata
     7801 atcagaaatg ctcagggtgg atttgatgaa ccgctggtta atgctgttat cttcagacaa
     7861 aacactgaag gactttatgg aggtgttgaa tggacaaatc ctccaaagca ggttcgcgat
     7921 gctttagaga ctcatccaaa aatgaaattc tttaaagatg tttcaagtga agacatggca
     7981 atctctacaa gaattgttac acgaaatgca tcacgcagaa ttattcgcgc tggttttgag
     8041 tatgcaaaaa aatatggcta tacaaatctt acactctgcg aaaaaccaaa cgtacttcgc
     8101 gaaacttccg gtatgatgtt taaagtagca aaagaaattg ctaaagaata tcccggtatt
     8161 gctttgtggg ataccaatat tgatgcacaa atgatgtggc ttacaaaaaa tccggaaaca
     8221 tatggagtta tagttgcgga aaatatgttt ggagatatta tatctgatgg ttttgccgga
     8281 ttggttggcg gacttggttt tgcctgcagt gcaaatattg gtgaagaggt tgcggttttt
     8341 gaacccacac atggaagtgc accaaaatat caggaattaa atccatcaat cgtaaatcca
     8401 attgcaatga tgttaagcgc ttgtatgatg ctcgatcaca ttgatgaaaa acctaaagct
     8461 gataaaatca gaagtgcaat tgcaaaagtt gttgaagaag gtaaagttaa aacttacgat
     8521 atgatgaagt tgcgcggtgg tcctaatgta tttagtaaag gtgcctgcac aactcagcaa
     8581 atgactgacg aaatcattaa aaatctataa ataattaagt cattggaagg accgaatcaa
     8641 tctgtctgtt ctgatagatt gcttcactcg aaaactcgtt cgcaatgaca atcgataaag
     8701 aatgaacgga atagaattag taatcatcgc tttggtattg tatgttgctg catacaagtt
     8761 gtacggtggg tttttatcca ggcgacttga ggtaaataac aaagtagaaa ctccagcaca
     8821 cacaatgtcg gacggtgttg attactgtcc tgcaaaagct ccggttttac ttggtcatca
     8881 ttttgcttca atcgctggtg caggtccaat cgttggtccc gtaatagcag ctgggtttgg
     8941 ctggatgccg gtttaccttt gggttgtatt tggtgcaata tttatcggag gtgtacacga
     9001 ctatgcaagt attattgcat cggttcgcca tcaaagtaaa tcaattggct atattattca
     9061 gtcttacatt ggtattagtg gaaaaaaatt atttctgatt tttgcctggg ctactttaat
     9121 tcttgttatc gctgtcttta caataattgt tgctgatact tttactcata tcccaagtgc
     9181 cggaacatca tcaattctat ttatgtttct cgcagtgtta tttggaattg caatttacag
     9241 gttaaagatt cgactttgga ttgcaacaat gattggtgtg attttacttt ttttatcgat
     9301 tcccgctggt aatttgtttc caatccaatt aagtgcatta acatggcagt taattttgtt
     9361 tggatacatt tttattgctt caactgtacc tgtctggata ttgcttcaac ccagagatta
     9421 cttaaactca ttttttcttt atgctttaat ggcgggcggt ttaatcggag ttttcttcgc
     9481 agcaccatca attaatattc ctgcattcag tactttttca ttagataaag ttggatacct
     9541 atttcctgct ttatttgtta cggttgcctg tggagcaatc agcggatttc attccatagt
     9601 tggaagcgga acaacagcaa agcaactcaa caaagaaact gatggaagaa ttattggtta
     9661 tggtggaatg ttgattgaag gaatgttagc tgttttatca ctcattgcag tagcatcaat
     9721 ggtaaataaa gaatttattg atattcttgt tactaaaggt ccggtgcctg cattttcact
     9781 tggtgttgca agatttataa atgcaattcc tgtgttgaat atttcaattc cgatggcgca
     9841 aacatttacg gctttagcag tctctgcttt tgcactcaca tcactagata cagctacacg
     9901 tttagcaaga tttatgtttc aggaatattt tgaaaataaa gagagtgaaa aaaagtttgt
     9961 attggttacg aacagatatg tatcaacagc aataactgtt gtgtttggtg cggcacttac
    10021 tttcagcggg caaacgatgt caatctggcc tgtgttcgga agtgcaaacc aattacttgc
    10081 cgcgcttgca ttacttgcgt tgactgtttg ggtcgctaat cttaaaaaag gatatttatt
    10141 tgttctcatt ccaatgatct tcatgtttgc cgttacactt accgcacttg gaatgctaat
    10201 ctatacaaat tttatcgcta ctaattatac attatcgatt atctcaattt tattgttcac
    10261 tttagctgtc ctgcttggat taaaagcgta cagtgtttta actaacggaa aagaactaag
    10321 taacgaacct gcataacgga gaaaattaat gaaagaaaaa gttttaaaga tatggccaga
    10381 aataaactgg atagaaaatc aagcgctcag agaaaaagtt ttaaattgct gggtttatgc
    10441 aattgaaaat tcggtgttat ctccggaaga tttagaagta attccattct cacttcttat
    10501 taaagattgc aaaatatcct ttatgaatca taaacgcaca gcagtgcaac tttctgttga
    10561 tattgcaaaa cgaatgaaag caaattttaa agaagaaata aaaatagata tggatttatt
    10621 aattgctggt gcaatcttaa tcgatattgg aaaattaatt gaatatgata aagttgatgg
    10681 aaaactcgtt acaagtaaag caggtacttt gttacgtcat ccgtttagcg gagtatcaat
    10741 tgctgataga ttcggattac cgtacgaagt gcagcatatc attgcttatc acgcaaaaga
    10801 aggtgatctt gcaaaacgta atgttgaagc aataatagtt catcatgcgg attttgtttc
    10861 attcgatccc tttaaagctt aggaggtaaa atggcgcaaa cactaattgg aaaaatagca
    10921 caaaaatttg ctgtgggatt ggacccaaag cataaggtcc aagcaggtga ttatctttcg
    10981 ataaaacctg cttatgtgat gacgcacgat aacactggtg cggtaatacc aaaattcaaa
    11041 tccattgggg caacaaaact ttttaatccg agacaagttg taaatactct tgatcataac
    11101 gttcaggata aaactgaaaa gaatttagag aagtataaaa agattgaaaa cttcgctaaa
    11161 tcgatgggag ttgattttta tcctgccgga cgtggaatcg gacatcagat tatgtgtgaa
    11221 gaaggatatg catggccggg aacaatggtt gtagcttctg attcacattc aaatatgtat
    11281 ggcggacttg gctgtctcgg aactccgatt gtccgaactg atgccgcagc aatttgggca
    11341 acaggaagaa catggtggca ggttccccct atcgcaaaag ttgaattaaa gggaagttta
    11401 agacaaggtg ttactggtaa agatgttata attgctctgt gcggatattt taatcacgat
    11461 gaagtattga accacgcaat agaatttgtt ggtgatggaa ttcgatatct gacaattgat
    11521 gaaagattag caatagcaaa tatgacaact gaatggggtg cacttgccgg tgtttttcct
    11581 atcgatcttg cgacaattct atggcttaaa aaacgagaaa ggtttataga acaccgtgga
    11641 cttgaaggtg ttttttctga tacagatgga aaaggagaac atccaagaat aaatcacaaa
    11701 cgaattgaag aacttgaaca caatatacca caagcggatg ctgatgcttt ttattcaaaa
    11761 gaattaatta ttgatctttc tacaattgaa ccttttgtgt ccggaccaaa tactgttaaa
    11821 acctatgcag cagtatccga attaaaagaa aaaaatattt caatcaacaa agcttatctt
    11881 gtttcttgtg ttaatagccg ggttgatgat attgctgaag ctgctgctgt tgttaaaggt
    11941 aaaaaagttg cagagcatgt aaagttttat attgcagcgg catcaagtga agtgcagaaa
    12001 gaagctgaac g
//
LOCUS       DASWBY010000588         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_620311, whole genome shotgun sequence.
ACCESSION   DASWBY010000588 DASWBY010000000
VERSION     DASWBY010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620311"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VF870_10680"
     CDS             <1..711
                     /locus_tag="VF870_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9252698.1"
                     /translation="ALGKVYTFGPTFRAEKSKTRRHLTEFWMVEPEVAFNDLNDNMDL
                     AEEFLEYIVQTILKEKSEELKVLERDITKLQNVKRPFPRVHYDEAVEILKKNGVDFQW
                     GNDLGGADETIVSEQYDRPIMVHHYPAEVKAFYMKRDPDAPKYALAVDVLAPEGYGEI
                     IGGSQREDNLDFLLERIKEHNLPQSAFEWYLDLRRFGSVPHAGFGLGLERTVSWICGL
                     DHLREAIPFPRMIYRNTP"
     gene            763..1278
                     /locus_tag="VF870_10685"
     CDS             763..1278
                     /locus_tag="VF870_10685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9252699.1"
                     /translation="MELNSILFFVFGFIAAVTAVILITRSNPVISALFLVLNFASLAG
                     LYLTLNAQFIAVTQIIVYAGAIMVLFLFVLMLLRPENEKKFLELNPKIKIFALSVAFI
                     LFAQLAYIIFFAEPSKNISPNLAKSVESGTIEAIGRQLFTNYIVPFEAAGFLLLAATI
                     GALVLAKKKFD"
     gene            1291..1596
                     /gene="nuoK"
                     /locus_tag="VF870_10690"
     CDS             1291..1596
                     /gene="nuoK"
                     /locus_tag="VF870_10690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX9252700.1"
                     /translation="MTIPVEFYLVLSAFMFFVGVAGVLTRRNAIIVFMSIELMLNSAN
                     LTLVAFSSYLGNSIGQLFVFFVMTVAAAEAAVGLAIIIAIFRNKLTVNIDEINILKW"
BASE COUNT          555 a          241 c          333 g          606 t
ORIGIN      
        1 gcattaggca aagtttatac atttggaccg actttccgtg cagaaaaatc aaaaacaaga
       61 agacatttaa cagaattttg gatggttgaa cctgaagttg catttaacga tttaaatgat
      121 aatatggatc tggcggagga gtttttagaa tatatcgttc agacaatttt aaaagaaaaa
      181 tcagaagagc tgaaagtttt agaaagggat ataacaaaac ttcagaacgt aaaacgtcct
      241 tttccacgag ttcattatga tgaagcagta gaaattttga aaaagaacgg tgttgatttt
      301 cagtggggca acgatttagg cggtgccgat gaaacaatag tttccgagca atatgataga
      361 ccaattatgg ttcatcatta tcctgcagaa gtaaaagcat tttatatgaa acgtgatccc
      421 gacgcgccaa agtatgctct tgctgttgat gttcttgcac ctgaaggcta cggagaaatt
      481 attggaggca gccagagaga agataatttg gattttcttt tagaaagaat taaagaacac
      541 aatcttcctc aaagtgcatt tgagtggtat ctggatttaa gaagatttgg ttctgttcct
      601 cacgcaggtt ttggtttagg attggaaaga acagttagct ggatttgcgg attggatcat
      661 ttacgtgaag cgattccgtt tcctagaatg atttatagaa atacacctta gcaattaaca
      721 atgtacaatt ttgaatgtac aattaaagat tagaatattt aaatggaatt aaactcaatt
      781 ttattttttg tttttggatt tattgcagcg gttactgcag taatattgat aaccagaagt
      841 aatcctgtaa tctcagcttt atttcttgtt ttaaattttg catcactcgc gggattatat
      901 cttactttaa atgcacaatt tatagcagta actcaaataa ttgtttatgc tggtgcaatt
      961 atggtgttgt tcctttttgt tttgatgtta ctcagaccgg aaaatgagaa gaagttttta
     1021 gaattaaatc caaaaattaa aatctttgca ttgtccgttg cttttattct gtttgcacag
     1081 cttgcttata taatattttt tgcggaacca tctaagaaca tttctccgaa tttggcaaaa
     1141 agtgttgaat caggaaccat agaggctatc ggcaggcagt tatttacaaa ttatatagtt
     1201 ccatttgaag ctgcaggatt tttattatta gcagcaacaa taggtgcatt agttttagca
     1261 aaaaagaaat ttgattagag aagtttaatt atgacaatcc cagttgaatt ttatttagtc
     1321 ctaagtgcat ttatgttttt tgtcggagta gccggcgtac ttacaagaag aaatgcaatt
     1381 atcgttttta tgagtatcga gttaatgctc aactcagcaa acttaacatt agtagcattc
     1441 tcatcttatt taggaaattc aattggccag ttgtttgtgt tttttgtgat gacagttgct
     1501 gcggcagaag ctgctgttgg gcttgcaata attattgcaa tcttccgtaa taagcttact
     1561 gttaacattg atgaaataaa tattcttaaa tggtagttta gttttttgtc attccgaact
     1621 tctttcggaa tcttaattta ttattcggaa gaagctgaaa taatttcagc aagacattaa
     1681 aaatatttga aatcttaaat tatttatgcg tgaattaatt tatttaaccg ttgtt
//
LOCUS       DASWBY010000589         9643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_322011, whole genome shotgun sequence.
ACCESSION   DASWBY010000589 DASWBY010000000
VERSION     DASWBY010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322011"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VF870_10695"
     CDS             <1..1285
                     /locus_tag="VF870_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9252701.1"
                     /translation="MLLFFIIFPKYFLGIAFSDSQQTALFIITFSILVYNIIFHFGRQ
                     YLKHDADSFNPLHLSILQMISDLIALMLVVYFTGSVESPLLLFFVIHMIVGSLILPGF
                     VIYTFAIAIVLTFWGITVGEYYSILPHHHVSGFLLFPLHKQFNYVLAINVSFAFLVDM
                     IVLIANRMAKQLYIREQQLLESIDKINSAEKEKQKYISGIVHEIKTPLSAVHSYLDLV
                     LQKFLGPLDEVVEEKLIRARRRSEEAIELINNVLKISKLRIEDSFIKEKVDITPILAR
                     AIRSAKVNADAKGVKLSIVKHVKGKHKIEGDPLLIQIALSNIISNAIKYNNMDGVVEI
                     EVDEDKNDLIIIVSDNGVGIPKEEIDKIFNDFFRASNIKRSGVEGSGLGLSVVKQIVE
                     RHGGTIDVHSPSNLSTNKYPGTCVKITLPFLKEQA"
     gene            complement(1254..1553)
                     /locus_tag="VF870_10700"
     CDS             complement(1254..1553)
                     /locus_tag="VF870_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252702.1"
                     /translation="MKTILLILIGQRKQATVQVQKVLTGWGCTIKTRLGIHDSVMENC
                     SDQGLLILELAGERKEMDELARKVSLIKGVSSKLIDLKIPKEKNIVKPAPSKKVK"
     gene            1831..3189
                     /locus_tag="VF870_10705"
     CDS             1831..3189
                     /locus_tag="VF870_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX9252703.1"
                     /translation="MKIFKPVFISLLLILVFTFNLPAQTKAGADALGSRKYEDYKTPE
                     YCGSSCHVDFYQQWKQAMMSQAYTHHWDEIEYFKLAVPHAEKDPVVAEVKAGCNGCHA
                     PISFLVGDVPPPKPEMNSRANESVSCDLCHTVTGFSGDTPFNFNFVSEPGNVKYGPRE
                     NVESPEHEIKKSEFLQTAEFCGTCHNEKDPYGIWVKSTHLEWKEGPYSKQGVQCQDCH
                     MTKAEGYTAAMGNQYPDAWQHLFHGAHDPGKVKGTIELRIHPDIREAEPGETVKFTVA
                     LFNQKTGHKFPTGSVEDRILWMHVEAVDAKGNVYHLPVNKKGFEGEEYTIGADALAYQ
                     DMGIALGDPNFKGVQRDGIPIGDRIFRKPYFDPQGRMTIQQWNTKSQGVDYRIGPRET
                     KMEYFTFNLPNDIAPGQLKVTAVLNYQKLIKPVADFLGVPPDEAEIIKVNEHTTYVTV
                     LP"
     gene            3332..4519
                     /locus_tag="VF870_10710"
     CDS             3332..4519
                     /locus_tag="VF870_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252704.1"
                     /translation="MKKIFTLAVLAFVITSFMVSDLFAIPAFARKYNMTCKTCHSPFP
                     SLKPYGEAFAFNGFQLKDNDAPRFYVDTGDPELTLIRDLPLALRLEGYVTYNQGNTEQ
                     SDFTSPLAFKLLSGGAITKDVAYYVYYILESGEVGKIEDAWFMFNNLFNTELDFLIGQ
                     FQVSDVLFKRELRLTRDDYYIYKVKPGFSNADLTYDRGIMLSYGFSTGTDITFQVING
                     NGIGESPFAGNFDNDKYKNLFGRISQDVGEHFRIGALGYWGKEKTMEQTEPNDATNEL
                     WMLGGDATISFDPIELNLQYVERNDNNPYFQYNNTNPIPEVATRGAFAEMIYRPDGDV
                     SKWYAVALFNWIESDQSDLNYRTLSGHFGYLLRRNIRLVGELNYDFNNEYATIGVGFV
                     TAF"
     gene            4704..5669
                     /locus_tag="VF870_10715"
     CDS             4704..5669
                     /locus_tag="VF870_10715"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX9252705.1"
                     /translation="MTSAKKEFQFSNTPEPHKERTRAILKAHPEVREHIGRNPYSFLI
                     ILFVVGLQITIAYFLSSQPWWLALLAAFFIGAFANHACYVLIHEAAHNLIFKNRPMNF
                     ISGILADIPNVLPSSVSFRSYHLKHHSFQGDYYLDADLASKWEAKIIGNSFFGKALWE
                     VLFPVFQAVRTPRLKEIQFMNMWTIINWIVVFGIDALVVIFFGWTSFLYLVFSFFFSI
                     GFHPLGARWIQEHFLIAPPQETYSYYGPLNTVALNVGFHNEHHDFPSIPWNNLPKLKA
                     TAPEFYDNLVYHKSWTKLWLKFLFDPNLSLYSRMTRDNRGGLSVE"
     gene            5815..6306
                     /locus_tag="VF870_10720"
     CDS             5815..6306
                     /locus_tag="VF870_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252706.1"
                     /translation="MPEKLLCDFCGTEITNDTEFCLKCGTIFIDDVTCFNHSDDDAKG
                     VCAICHQAYCKKCGLRVNGIFLCNEHSNYEIYEGMARVFGSSDEQQVNNYKSGLEQND
                     LHPFIFQRKASPISLGGGDYTLFRASGDAGGHIINEIKLMVPCSEVLQAEKIIDELEQ
                     SNY"
     gene            6392..7270
                     /locus_tag="VF870_10725"
     CDS             6392..7270
                     /locus_tag="VF870_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9252707.1"
                     /translation="MIYRNFGNTGFKVSALGFGAGEIGDYEVSEKDSENILNTVLDLG
                     INLIDTARGYYASEERTGKYISNRRKEFILSTKVGYGIEGYADWSYDIILAGVDNALR
                     LMKTDYIDIVHLHSCDLNTLQRGEVIEALHKTIEQGKVRIAAYSGENEELKFAVDSNS
                     FDSVQTSINICDQIDLDGSIKQAKEKGMGIIAKRPIANAPWRFSERPVGHYCEEYWHR
                     WKTMNLPEQKNWLDTFLRFSVFANGVDTAIVGTTSIEHLKSNIKIIEQGPLDDELNNL
                     IIESFKTHNNNWRGEV"
     gene            7568..7804
                     /locus_tag="VF870_10730"
     CDS             7568..7804
                     /locus_tag="VF870_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX9252708.1"
                     /translation="MIKGTVIIDGKICKGCELCIVACPQEGLALSASFNEKGYRFVEL
                     INDKCTGCVNCALVCPEAAITVYREPKPAKKVQA"
     gene            7831..8925
                     /locus_tag="VF870_10735"
     CDS             7831..8925
                     /locus_tag="VF870_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HEX9252709.1"
                     /translation="MENKEKKSGEARLMKGNEALAEAAIRADIDAYFGYPITPQSEVL
                     EYLEREIPNRHGLVLQAESEVASINMVYGAAGAGARVMTSSSSPGISLMQEGISYIAS
                     AQLPCLVVNVVRGGPGLGTIQPSQGDYFQATKGGGHGDYHLIVLAPASVQEMADFVFD
                     AFRLAEKYRNPAMILSDGALGQMMEKVILPEEGSLPKTPAPWATTGKTKNREHNIITS
                     LFIQPEEMEQVNLRLQEKYKAIQSEVRYEEIQTEDAEILLVAYGLSSRICQKTVDLAR
                     EKGIKVGLLRPITVYPYPYKRLNELADQVEFMLTVEMNAGQMVEDVRLAVEGKVPVHF
                     IGRMGGMILSPEDILHKIESVHESQNAVNV"
     gene            8937..>9643
                     /locus_tag="VF870_10740"
     CDS             8937..>9643
                     /locus_tag="VF870_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9252710.1"
                     /translation="MNEKLYLEDEENLGTQVDESYEQVYCRPDTLTDVPFHYCPGCGH
                     SVVHRVLMEVIAELGIQEETIGVAPVGCSVFAYNYMNIDMQEAAHGRASAVATGVKRV
                     LPDKYVFSYQGDGDLAAIGTAETIHTVNRGENILMLFINNGIYGMTGGQMAPTSLIGQ
                     VTSTSPYGREAAQVGNPIKIADMLCLLPGAYYVTRQAVHNPGGVRKLKKALVKAFEYQ
                     KQKKGTCFIEVVSNCPSG"
BASE COUNT         3114 a         1599 c         1901 g         3029 t
ORIGIN      
        1 gatgcttcta ttcttcataa ttttccccaa atatttttta ggtattgctt tctcagattc
       61 acaacaaaca gctcttttca taattacgtt ttcaatcctc gtttataata tcatttttca
      121 tttcggccgg cagtatctta aacacgatgc cgatagtttt aatccattgc atttgtcaat
      181 tcttcaaatg atttccgacc ttatagcact tatgcttgtt gtttacttta ccggaagtgt
      241 tgaatctccg ttacttttat tttttgttat tcatatgatt gtgggcagct taatcttacc
      301 aggatttgtt atctatacct ttgccattgc aattgttctt acgttttggg gcattacagt
      361 aggtgaatat tattcaatat tgccgcatca tcatgtgtca ggttttcttt tattcccgct
      421 tcataagcag tttaattatg tgcttgcaat aaatgtcagt tttgcatttt tggttgatat
      481 gattgttctg atcgcaaacc gaatggcaaa acagctttac attcgtgagc agcagctatt
      541 ggaatcaata gataaaataa attctgcgga aaaagaaaaa cagaaatata tttcaggcat
      601 tgtgcatgaa ataaaaacac cgctttctgc tgtacattct tatcttgatt tagtgctgca
      661 aaaatttctt ggacctttag atgaagtggt tgaagaaaaa cttattcgag ccagaaggcg
      721 atctgaagaa gctattgaat tgattaacaa tgtattaaaa atttctaaac tgcgtattga
      781 agattcattt atcaaggaga aagtggatat tactccaata cttgcaagag caattagaag
      841 tgcaaaagta aatgctgatg caaagggagt taaactatca attgttaagc acgtaaaagg
      901 aaaacataaa atcgaagggg atccgctttt aattcagatt gcactatcga acattattag
      961 caatgcaatt aagtataata atatggatgg agttgttgag atagaagttg atgaagataa
     1021 aaatgatttg ataattattg tctctgataa cggagttgga ataccaaaag aagaaattga
     1081 taaaatattt aatgattttt tcagagcaag caacattaaa cgaagcggag tagaaggctc
     1141 aggtttaggt ttatctgtag taaagcaaat tgtagaaaga catggtggaa caattgatgt
     1201 tcatagtcct tccaatcttt caacaaacaa atatcccggc acctgtgtaa aaattacttt
     1261 accttttttg aaggagcagg cttaacaata ttcttttctt taggtatctt taaatcaatt
     1321 aacttagaag aaacaccttt gattaaagaa acttttcttg caagctcatc catttctttt
     1381 ctttcgccgg caagttccag aatcaataac ccctgatcgc tgcaattttc cattacacta
     1441 tcatgaatgc ctaatcttgt tttgatcgtg catccccagc cggttaatac tttttgaacc
     1501 tgaacagtag cttgttttct ttgaccaatg agaattaata aaatggtttt cataaatccc
     1561 ccctgtaaaa tgtaaataaa atacgattta aaaatagaat aaaatctaga cactagtcga
     1621 taaaaataaa tttaaatgtt attaataaat tttttaatta tttttaaatc actcttaggg
     1681 gattaataaa tgtattttgt ttctggtttg ataaaaacta ttccacatac ttttttgtgg
     1741 tttaatataa accgcattca gtcctgttct gtgtccagtc cttcaaaaat ttcaattcat
     1801 tctatttcaa tattaatcag ggagattgct atgaaaattt ttaaaccagt tttcatttca
     1861 ttattactca ttttggtatt tacatttaat ctgccagcgc agaccaaagc aggtgctgat
     1921 gcccttggta gccgtaagta tgaagattat aaaacacctg aatactgcgg ttcatcctgt
     1981 cacgttgatt tttatcagca gtggaaacaa gcaatgatgt cacaagccta tactcatcac
     2041 tgggatgaaa ttgagtactt taaacttgca gtaccgcatg cagaaaaaga cccagttgtt
     2101 gcagaagtaa aagcaggatg taatggatgc cacgctccaa tttctttttt agttggtgat
     2161 gtgcctccgc caaaaccaga aatgaattca cgagcaaatg aatcagtatc ctgcgatctc
     2221 tgccatactg ttacgggatt ttcaggagat actccgttta attttaattt tgtttcagaa
     2281 ccgggtaatg ttaaatatgg tcctcgtgaa aatgtagaat ctccggaaca tgaaattaaa
     2341 aaatcagaat ttttgcaaac tgcggaattc tgcggaacct gccacaatga aaaagatcct
     2401 tacggcattt gggttaagtc aactcatctg gaatggaaag aaggcccgta ttccaaacaa
     2461 ggcgttcaat gccaggactg tcatatgaca aaagcagaag gttatactgc ggcaatgggg
     2521 aatcaatatc ctgatgcatg gcagcattta ttccatggtg ctcacgatcc cggaaaagtt
     2581 aaaggaacaa ttgaattgag aattcatcct gatattcgtg aagcagagcc tggcgaaact
     2641 gtaaaattta cagttgcatt gttcaatcag aaaaccggtc ataaatttcc aaccggatct
     2701 gttgaagaca gaatactttg gatgcacgtt gaagctgttg atgccaaggg aaatgtttat
     2761 cacctgcctg ttaacaaaaa aggatttgaa ggggaagagt atacaatcgg tgcggatgct
     2821 ttagcatatc aggatatggg aattgcttta ggggatccaa actttaaagg tgtgcagaga
     2881 gatggaattc ctatcggaga cagaattttt agaaaaccat atttcgatcc tcaaggcaga
     2941 atgacaatac aacaatggaa tacaaaatcg caaggagttg attacagaat tggaccgcgc
     3001 gaaacaaaaa tggaatactt tacttttaat cttccaaacg atattgctcc gggtcaatta
     3061 aaagtaacag cagtgcttaa ctatcaaaaa ttgattaaac ctgttgcaga ttttcttggt
     3121 gttccaccgg atgaagcaga aatcattaaa gtaaatgaac atacaactta tgtaacagtg
     3181 ctgccttagt tgataagtca ttgccggact cgatccggca tctatctctt aaattgttgt
     3241 caggttgagc ctgtcgaaac ctgacatatg ttttgtgaag tcacccttcg acaggctcag
     3301 ggtgacattt cgataaaata agaggattaa aatgaaaaaa atatttactc tcgcagtttt
     3361 agcttttgta attacatcat ttatggtaag tgatcttttt gctattccag catttgcaag
     3421 aaaatataat atgacttgca aaacatgtca ctctccattt ccatccctca aaccttatgg
     3481 cgaagcgttt gcattcaatg gttttcaact taaggataat gatgcaccac gtttttatgt
     3541 tgatacaggt gatcctgaat taactctcat cagagatttg cctctggcac tcagactaga
     3601 aggctatgtt acttacaacc agggaaatac cgaacaatct gattttacat caccattggc
     3661 attcaaactc ctttcaggtg gtgccatcac aaaagatgtt gcatactatg tttattacat
     3721 actggaaagt ggtgaagttg gaaaaattga agatgcctgg tttatgttta ataatctttt
     3781 taatacagag cttgattttt tgatcggtca attccaggtt agtgatgtac tttttaaaag
     3841 agaattaaga ctcacaagag atgattatta catttataaa gttaaacctg gtttttcaaa
     3901 tgccgatctt acttacgaca gaggaataat gctcagttat ggttttagta caggaactga
     3961 cataacattc caagtaataa atggtaacgg aattggtgaa agtccatttg ctggaaattt
     4021 tgataacgac aagtataaaa atttatttgg aagaatttca caggatgtcg gtgaacattt
     4081 tagaatcggt gcattaggat attgggggaa agaaaaaacg atggaacaaa ctgaaccaaa
     4141 tgatgcaaca aatgaattat ggatgcttgg cggagatgct acaataagtt ttgatccaat
     4201 tgaattgaat ttacagtatg tagaaagaaa tgataataat ccgtatttcc aatataataa
     4261 tactaatccg attcctgagg ttgcaactcg tggtgcattt gcagagatga tttatcgtcc
     4321 ggatggagat gtaagtaaat ggtatgcggt cgcattgttc aactggattg aatcagatca
     4381 gtcagatctt aattatagaa ctctttcagg tcattttggt tatttgctca ggcggaatat
     4441 cagattggta ggcgaattga attatgattt taataatgaa tatgcaacaa ttggtgttgg
     4501 ttttgtaaca gcattttgat taagagctat tatttcctgt cattccgagc gtagtcgagg
     4561 tagaaaggat aatattacat ttttagttgt cacacttcga ctgcgctcag tgtgacaaat
     4621 cttttcttac ttcacaaata agtttcaaaa ttttatcgta attttgttta attaaagagt
     4681 caaatattaa aaggcgactt aaaatgactt ctgcaaaaaa agaatttcag ttctctaaca
     4741 cacccgaacc gcacaaggaa agaacacgcg caatcttaaa agcacatcct gaagtgcgag
     4801 aacatattgg tagaaatcca tatagttttt tgataatact atttgttgtt ggattgcaaa
     4861 tcacaattgc ttatttcctt tcgtctcagc cgtggtggct ggcattgctt gctgcttttt
     4921 ttataggtgc gtttgcaaat catgcttgct atgttttgat tcacgaagcg gcacataatc
     4981 taattttcaa aaacagaccg atgaatttta tctccggaat tcttgcagat attccgaatg
     5041 ttctgccgag ttctgtttct ttcagaagct atcatttaaa acaccattcg tttcaaggtg
     5101 attattatct tgatgcagat cttgcaagca aatgggaagc aaaaattatc ggtaattctt
     5161 ttttcggaaa agctttatgg gaagttctct ttcctgtatt ccaggctgtt agaactccac
     5221 gtttaaaaga aattcagttt atgaatatgt ggaccatcat taattggatt gttgtttttg
     5281 gtatcgatgc actggtagta atattctttg gctggacttc atttctttat cttgttttct
     5341 ctttcttctt ctcaatagga tttcatcctc tcggtgcaag atggatacaa gaacactttc
     5401 ttatagctcc gccacaggaa acctacagct attatggtcc gctaaacact gttgctttaa
     5461 atgttggatt tcataatgag catcatgatt ttccatcaat tccatggaat aatcttccca
     5521 agctaaaagc aacagcaccg gaattttatg ataatctagt ttatcataaa tcgtggacaa
     5581 aactttggct taaatttttg tttgatccaa acctttctct ttactcaaga atgaccagag
     5641 ataacagagg gggattatcg gttgagtaaa atttcctttt ttaaatattt ttatagttaa
     5701 atttttgaga tgctcgatcc ttgattctgg taaaataaaa ttttacatat aatcaattga
     5761 ccagtgccca gaatcaagaa tcctgtattc agaatccagc aatcaggaga aaaaatgccc
     5821 gaaaaacttt tatgtgattt ctgtggaaca gaaataacta atgatacaga gttttgttta
     5881 aagtgcggaa caatttttat agatgatgta acctgcttta atcattccga tgatgatgct
     5941 aaaggtgtgt gtgcaatctg tcatcaggct tactgcaaaa aatgcggatt gcgggttaac
     6001 ggcatattct tatgtaatga acattctaat tatgaaatat atgaaggtat ggctcgcgtg
     6061 tttggttcat cggatgagca gcaagttaat aattataaat ccggtttaga acaaaatgat
     6121 cttcatccat ttatatttca acgcaaagca tcgccaattt cattaggtgg cggagattac
     6181 actctgttcc gtgccagcgg tgatgcgggt ggtcatataa ttaatgagat aaaattaatg
     6241 gttccttgct cagaagtgct tcaggcagaa aaaattattg atgagctgga acaatcaaat
     6301 tactaacaat cgtaacgcag acttcagtct gcgaaataaa taattacaat tcacaatctc
     6361 agactaaagt ctgagctaca gaaaatgaat tttgatatat agaaattttg gaaatacagg
     6421 atttaaagta tccgcactag gttttggtgc gggagagata ggggattatg aagtttctga
     6481 aaaagattct gaaaatatcc taaatacagt attagatctc ggcattaatc taatagatac
     6541 tgcccgcgga tattatgcaa gtgaagaacg gactggaaaa tatatttcca acagaagaaa
     6601 agaatttatc ctttccacaa aagttggata tgggattgag ggttatgctg attggagtta
     6661 tgatataatt cttgccggag ttgataatgc tttgcgatta atgaaaactg attacattga
     6721 tattgttcat ctgcattcct gcgatttgaa tactttgcag cgcggagaag ttattgaagc
     6781 acttcacaaa accatcgaac agggaaaggt tagaattgca gcttattccg gtgaaaacga
     6841 agaattaaaa tttgcagtcg attcaaactc tttcgacagt gttcaaactt caattaatat
     6901 ttgtgatcag attgatcttg atggctctat caaacaagca aaagaaaagg gaatgggaat
     6961 tattgcaaaa cgccctattg caaacgcacc ttggagattt tctgaaagac ctgttggaca
     7021 ttactgcgaa gaatattggc atcgctggaa aaccatgaat ttacctgaac agaaaaactg
     7081 gctggatacg tttttaagat tttcagtttt tgcaaatgga gttgatacag caattgttgg
     7141 aacaacaagt attgagcact taaaatctaa cataaaaatt attgaacaag gtccgcttga
     7201 tgatgaactt aataatttga ttatagaaag ttttaaaaca cataataata attggcgtgg
     7261 cgaagtttag atcattgccc attgtcatcc tgagcgaagt cgaaggatga ccgtttccca
     7321 ggatttgggt aaactcacac ttcgactacg ctcagtgtga cggtagaatg taaacaaatc
     7381 cattcaataa ttgctaactg acaaaacctt ctcataaata ggaatcaccc ttcttttagg
     7441 cttcttaaat ctgataatta aaccattttt tagtgccttt aagcgctcta acctcattta
     7501 tcatatggca ttaagattga gattttgact ataattaaaa gaattctcaa atccgaaaga
     7561 gacagaaatg ataaaaggaa cagtaataat cgacggtaaa atttgcaaag gatgtgagct
     7621 ttgcattgtt gcttgtccac aggaagggct tgcattatcc gcaagtttta acgaaaaagg
     7681 ttaccgtttt gttgaactga ttaatgacaa atgtacaggt tgtgttaatt gtgcacttgt
     7741 ttgtcctgaa gcagcaataa cagtttatcg cgaaccaaag cctgctaaaa aagttcaggc
     7801 ttaagtttaa ttaattacaa aaaggttttt atggaaaata aagaaaaaaa gtcgggtgaa
     7861 gcccgattaa tgaaaggtaa tgaagcactt gccgaagcag cgattcgtgc agatatagat
     7921 gcgtattttg gttatccgat tactccgcag tcagaagttc tggaatatct tgaaagagaa
     7981 attccaaaca gacacggatt agttttgcag gcagaaagtg aagttgcttc aatcaatatg
     8041 gtttacggtg ccgcaggtgc cggcgcaaga gtgatgactt catcctcatc accgggaata
     8101 agtttaatgc aggaaggaat ttcttatatc gcatcagcgc agcttccttg cctggttgtt
     8161 aatgtagttc gtggtggtcc aggattggga accatccagc cttctcaagg tgattatttt
     8221 caggccacaa agggaggtgg tcatggcgat tatcacctta ttgttttagc tcctgccagt
     8281 gttcaggaaa tggcggattt tgtttttgat gcttttagat tagcagaaaa atatcgtaat
     8341 ccggctatga tactttctga tggtgctctt ggtcagatga tggaaaaagt aattcttcct
     8401 gaagaaggat cgcttcccaa aactcctgca ccttgggcta caaccggtaa aacaaaaaac
     8461 agagagcata atattatcac ttctcttttt attcagccgg aagaaatgga acaggtaaat
     8521 cttcgtctgc aggaaaaata taaagcgata caaagcgaag tcagatatga agaaattcag
     8581 acagaagatg cagaaatatt attagttgct tatggtttat catcacgtat ttgccagaag
     8641 acagtggatc ttgctcgcga aaaaggaatt aaggttggat tgctgagacc tattactgtt
     8701 tatccatatc cttataaaag attaaatgag ttggctgatc aggttgaatt tatgttaact
     8761 gttgaaatga acgcaggaca aatggttgaa gatgttcgtc ttgctgttga aggaaaagtt
     8821 cctgttcatt ttatcggaag aatgggggga atgattttat ctcctgaaga tattcttcat
     8881 aagattgaat cagttcacga atcacaaaat gcagttaatg tttaggagaa aatgaaatga
     8941 acgaaaaatt atatttagaa gacgaagaaa atttaggaac tcaggttgat gaaagttacg
     9001 aacaggttta ttgcagacct gatacattaa ctgatgttcc gtttcactac tgccccggct
     9061 gcggacacag tgttgttcac agagttttaa tggaagttat tgcagaactt gggatacagg
     9121 aggaaacaat aggtgttgcg ccggttggat gttctgtatt tgcatataac tatatgaaca
     9181 ttgatatgca ggaggccgct cacggaagag caagtgctgt tgcaacggga gtaaaaagag
     9241 ttctgcctga taaatatgtt ttctcatatc agggtgatgg cgatcttgcc gcaattggaa
     9301 cagccgaaac aatccatact gtaaaccgcg gtgaaaacat tttaatgctt tttatcaaca
     9361 atggaattta cgggatgaca ggcgggcaga tggctcccac gagtttaatc ggacaagtaa
     9421 cctctacgtc tccttacgga agagaagctg cacaggttgg caacccgata aagattgccg
     9481 atatgctttg cttgttgcca ggtgcatatt atgtaacaag gcaggctgtt cataatcccg
     9541 gaggagtacg aaaactaaag aaagcattag taaaagcatt cgaatatcag aaacagaaaa
     9601 aaggaacatg ttttattgaa gttgtttcca actgtccatc agg
//
LOCUS       DASWBY010000590         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_394650, whole genome shotgun sequence.
ACCESSION   DASWBY010000590 DASWBY010000000
VERSION     DASWBY010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394650"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VF870_10745"
     CDS             complement(<1..1306)
                     /locus_tag="VF870_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252711.1"
                     /translation="MKKYLNVSGTKLNSKIIWVLLGAGVILYALYILFISHTLTYDAH
                     PTDKQKILFVAQRILIGILFFIAILNINKLPLKETWIAWIIFTGLFARLILIPSSPIL
                     EDDFYRYLWDGAVTANEFNPYVFSPQDVIEKNQNAPQKILKLADESGNVIEKINHPKI
                     KTLYPTLSQIIFAFSYYLFPWSVTGWKLLMLMGDIFLLFFLIKILRELKLPVSFVAIY
                     WLNPIVLHEFFNTGHYDLFALLFTAIAIYYFLKDEFVTSSITLALAVGFKLWPILIFP
                     IFLRKLTNQKWKLVSSVFAFSIFVIVIFIPVLRAGFDQNTGFTKYAANWINNAAFYTL
                     LKNSIELFTSTFNIYYVCADCVARWITGGIILSVLLLLIKKPASDNLNLVYKMLIIIA
                     VSFLVSPTQFPWYVTWMILPLVFSPKISLLMYAFLIPLYHLDP"
     gene            complement(1317..2090)
                     /locus_tag="VF870_10750"
     CDS             complement(1317..2090)
                     /locus_tag="VF870_10750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9252712.1"
                     /translation="MIIEEIGKQKTVNGVIHLTEPDKSFEDLYLQTLKREKRLYSDDE
                     VKLLPYASKQNPHKDKWALRTKSFLRFKDYLSKKKSTLNILDLGCGNGWLTAQLAKEF
                     DHNYFCIDTNLVELEQAARVFSKDNISFFYADITKASFPSNTFHIIIINSYLQYFPEV
                     ENLLKECFTISKSYGEVHIIDTPFYKENEIMQARNYSLKHFTSIGLPEMTKKYHHHTI
                     KELKYFRYRYLYNPDLLKNKLTNFIFEKDSPYPWIMITR"
BASE COUNT          879 a          370 c          386 g          787 t
ORIGIN      
        1 aaggatctaa atgataaagc ggaattagaa acgcatacat tagtaaagat atttttggac
       61 tgaagacaag cggcaatatc atccacgtaa cataccacgg aaattgcgtt ggacttacaa
      121 gaaatgaaac tgcaattatt atcagcattt tgtaaacgag gttaagatta tcgcttgcag
      181 gttttttaat aagcaataat aaaacagata aaattattcc acctgttatc cagcgggcaa
      241 cacaatccgc acacacataa tatatattaa aagttgaagt gaataattct atactgtttt
      301 ttaaaagagt ataaaacgca gcattgttta tccagtttgc ggcatacttt gtaaatcctg
      361 tattctgatc gaagccggca cgtaaaacag gaataaatat tacaataaca aaaattgaaa
      421 aagcaaaaac actggaaacc aatttccatt tttgatttgt cagttttcta agaaaaattg
      481 gaaagattaa aatcggccaa agcttaaatc ctactgcaag tgctaatgtt atacttgatg
      541 ttacaaactc atcttttaaa aaataataaa tagcaattgc tgtaaaaagc agtgcaaata
      601 aatcataatg tccggtatta aaaaattcgt gcagtacaat tgggttaagc cagtaaattg
      661 caacgaaaga aacaggcagc ttaagttccc gcaatatttt aattaaaaag aaaagtaaaa
      721 aaatatcacc cattaacatt aacaatttcc aacccgttac actccagggg aaaaggtaat
      781 atgaaaaagc gaatatgatt tgagaaagcg ttggataaag tgtttttatt tttggatgat
      841 ttatcttttc tataacattt ccagattcat ctgctaactt taaaatcttt tgtggtgcat
      901 tttgattctt ttcaataaca tcctgcggac tgaatacata aggattaaat tcattcgcgg
      961 ttacagcacc atcccataaa tatctataaa aatcatcttc gagaattgga gaagagggga
     1021 tcaaaattaa tcttgcaaac aatccggtaa agattatcca ggcaatccag gtttctttta
     1081 gaggaagttt attaatgttt aagatcgcta tgaaaaaaag aattccaatt aaaattcttt
     1141 gagcaacaaa cagaattttc tgtttatcgg taggatgtgc atcgtaagtg agagtatgtg
     1201 atatgaaaag aatatataaa gcgtaaagga taactcctgc tccgagaagt acccagataa
     1261 ttttactgtt taattttgtt ccagaaacat taagatattt tttcatagct gtctgtctac
     1321 cgtgttatca ttatccaggg gtaaggggaa tccttttcaa agataaagtt agtcaatttg
     1381 ttttttaaca aatcagggtt gtacaaatac cgatatctga aatattttaa ttctttaatg
     1441 gtgtgatgat gatatttttt tgtcatttcc ggcagcccaa ttgaagtaaa atgttttaaa
     1501 gaataatttc tggcctgcat tatttcattc tctttataaa acggtgtatc aataatatga
     1561 acttccccat aagattttga aatagtaaaa cactccttca ataaattttc aacttcagga
     1621 aaatactgta agtaagagtt gattataatt atgtgaaaag tgttcgatgg aaaagatgct
     1681 ttcgtaatat ccgcgtaaaa aaagctgata ttatctttag aaaacactcg tgcagcctgc
     1741 tctaattcaa ctaagtttgt atcaatgcag aagtaattat ggtcaaactc ttttgctaat
     1801 tgagctgtta gccatccatt tccgcagcca agatcaagaa tatttaaagt cgatttcttc
     1861 tttgataaat aatctttaaa tcgcaaaaat gattttgttc ttaatgccca tttatcttta
     1921 tgaggatttt gtttagatgc atagggtaac agcttaactt catcatcact ataaagtctt
     1981 ttctctcttt ttaaagtttg taaatataaa tcttcaaagg acttatccgg ctcggttaga
     2041 tgtataaccc catttacagt tttctgttta ccaatttctt caataatcat attgtaaaat
     2101 aaatatatgt taatcaaaaa tcagaatagc agctaatatt agttgtatac tgggaaattt
     2161 ttcaaattaa ctttaaattt tacaaatatc acaaatgggt ttaattagaa aacagagatc
     2221 atttccttat ttttaattat taaaaattta gaaatttcca ttgtgattca gaaagagagg
     2281 aaaaatggaa aagcttttat tacttggtgc agaagcaata gcacaaggcg ctattgacgg
     2341 gggaatgtct ggcatttatg catatcccgg tacgccttcc acagaaataa ccgaatacgt
     2401 tcaaaacaat aaaattgcaa ca
//
LOCUS       DASWBY010000591         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_174313, whole genome shotgun sequence.
ACCESSION   DASWBY010000591 DASWBY010000000
VERSION     DASWBY010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174313"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="VF870_10755"
     CDS             complement(<1..1552)
                     /locus_tag="VF870_10755"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEX9252713.1"
                     /translation="MKIFFISFLMIVALTSTTKSQTESVTFNSSIDGSLIEFTSSNLP
                     IVIINTNGQEIPDDYKITADMGVIYNGEGVRNSLTDPYNNYNGKIGIEIRGSSSQQFP
                     KKQYAVETRDALGNDLDVSLLGFPQESDWILFAPYNDKSLMRDVLVYKIASSMGRYAS
                     RSKYCEVVLNGEYIGVYVLLEKVKRDANRVNIKKMDPTDISGDAVTGGYIIKIDKTDG
                     ENNDGWYSTYLPFPQAQYSVFYQYHVPKPDEIVQQQKDYIQNKIFQFETMMALAINIT
                     DSATGYPKYLDTDSFVDFLIVNEVAKNVDGYRLSTYLYKDRDSRNTKIFAGPVWDFNL
                     GFGNADYYEGWQTTGWQLQFLTDYQNMPSYESFLTPNWWRKLFDDNAFRDKVYARWQS
                     VKSELINSQKINSYIDSLKVLLDESKTRNFQKWPVLGVWIWPNYYVGQTYEQEINYLK
                     NWIAFRLNWMNNNMIGHPTDVEGLLPQIPSEFKLEQNYPNPFNPDTKISWQSPVGGHQ
                     TLKIFDILG"
     gene            complement(1549..>1761)
                     /locus_tag="VF870_10760"
     CDS             complement(1549..>1761)
                     /locus_tag="VF870_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252714.1"
                     /translation="TIRYSLPKASNVTLKVYDIIGNEVSTLVNEFKVPGNYQIKFDAS
                     RLSSGVYFYRIKTEYFTSSKKLILLK"
BASE COUNT          553 a          330 c          255 g          623 t
ORIGIN      
        1 ttcccaggat atcaaatatt tttaatgttt gatgaccacc cactggagac tgccaactta
       61 tctttgtatc tgggttaaat ggattcggat aattctgttc tagtttaaac tctgatggga
      121 tttgcggtaa tagtccctca acatccgttg gatgacctat catattattg ttcatccaat
      181 ttaacctgaa tgctatccaa tttttaaggt agtttatttc ttgttcatac gtttgaccga
      241 catagtaatt tggccaaatc caaacaccga gcactggcca tttttgaaaa tttcttgtct
      301 tagattcatc aagcagaact tttaatgaat ctatataact gtttattttc tgagaattaa
      361 ttaattcgct tttaacactc tgccagcgag cataaacttt gtctctaaaa gcattatcat
      421 caaacaattt tctccaccaa tttggagtca agaaggattc gtatgagggc atattctgat
      481 agtctgttaa aaattgtaac tgccatccag tggtttgcca tccttcgtag tagtcagcat
      541 ttccaaatcc taggttaaaa tcccaaacag gtccggcaaa tattttagtg tttctactgt
      601 cccggtcttt ataaagataa gtacttaatc tatatccatc cacatttttt gcaacttcat
      661 taacaattaa aaaatccaca aacgaatctg tatctaaata tttcggataa ccggtagcag
      721 aatcagtaat gtttatagct aatgccatca tcgtttcgaa ctgaaaaatt ttattttgga
      781 tataatcctt ttgctgctga acaatttcat caggcttggg aacatgatat tgatagaaaa
      841 cagaatattg tgcttgcgga aaaggcagat aagttgaata ccatccatcg ttattttcac
      901 catcagtttt atctatctta attatgtagc caccggtaac tgcatctcca gagatatcag
      961 ttgggtccat ttttttaata ttaacacgat tagcatcacg ttttactttt tccaataaaa
     1021 catagacacc aatatattca ccatttaaaa ccacttcaca atattttgaa cgacttgcat
     1081 atctgcccat gctgctcgcg attttatata cgagtacatc acgcattaga cttttatcat
     1141 tatatggagc aaataatatc cagtcacttt cttgaggaaa tccaagaagt gaaacatcaa
     1201 gatcattacc aagtgcatct cttgtttcta ctgcatattg tttttttgga aactgctgag
     1261 aagacgaacc acgtatttca atacctatct ttccattata gttattgtaa ggatcggtaa
     1321 gagaatttct cactccctcc ccattgtaaa taactcccat atctgcagtt attttataat
     1381 catccggtat ttcctgtccg ttcgtattga ttataactat tgggagattt gaagaagtga
     1441 attcaattaa tgaaccatca attgaagaat taaatgtcac actttctgtt tgagatttcg
     1501 ttgtacttgt taatgcaaca atcattaaaa atgatatgaa aaatattttc attttagtaa
     1561 tattaatttt ttagaacttg taaaatattc tgtctttatt ctatagaaat aaactccgct
     1621 agataatcta ctggcatcaa attttatctg ataattaccc ggaactttaa actcattcac
     1681 taaagtactc acttcattcc caattatgtc ataaactttt aatgtaacat ttgaagcttt
     1741 aggcaatgag taccgaatag t
//
LOCUS       DASWBY010000592         1907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_478170, whole genome shotgun sequence.
ACCESSION   DASWBY010000592 DASWBY010000000
VERSION     DASWBY010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478170"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VF870_10765"
     CDS             <1..1294
                     /locus_tag="VF870_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252715.1"
                     /translation="NLQTEVNTSVVLAPTNNSSTEFVKKEVSSSKTDGRYSLQSYSNF
                     SNDGSSIDYQRWRHSLRFTSQRIGKSGFSFSTYAIFAYKADEWNVVSDNLNKAIKVYD
                     FNIKYEFDETSRILAGRFLNSKISNISTIDGAMVEKSFSSWTFGLVGGSRPDFSDFSY
                     NSKLFEYGGYISKSDTFGTAIMENTISAFNQTNDFKTDRRFLYFQHVNNLIKNTFLFG
                     SAEVDFFKIADSLAQNDFSLTGLFFSARYAPVREFSLNVSYDARRNIIYYETYKSLSQ
                     TILENELRQGFRARASFRPLTNLFFSLQYGYRNRKGDLKASTNYGANLGYSLVPLIES
                     SANINFNRINSSYVDGYVYSANLSKSIYALNSDLSLGFRKTIYNFSNGASQLEENAIL
                     IDYSINAFRAFSINISYEGVFESNHTYGRILFDITTRF"
     gene            1368..>1907
                     /locus_tag="VF870_10770"
     CDS             1368..>1907
                     /locus_tag="VF870_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX9252716.1"
                     /translation="MFEYVGSIHMHSTFSDGTGEVSDIAKFADEIGLDFIMLTDHNTL
                     RALQEGYEKWYGNTLLLVGCEINDKENKNHYLAFGIDEAFTTRTPAKKYVAKVKELGG
                     IGFLAHPHEKRKHKEHPAYPWVEWDTEDFTGIEIWNHMSEWVENLTEENKYRSFLHPL
                     RTIIAPPKETLKVWDELSQK"
BASE COUNT          645 a          285 c          340 g          637 t
ORIGIN      
        1 taatttgcag acagaagtaa acacttcggt tgtattagca cccactaata attcatctac
       61 cgagtttgta aaaaaagaag tatccagttc caaaactgat ggcagatatt ctttgcaatc
      121 atattctaat ttctcaaatg atgggagtag tattgactat cagcgttgga gacattcatt
      181 acgatttact tcacaaagaa ttggaaaatc aggattttca ttttcaactt atgcaatatt
      241 tgcttacaaa gctgatgaat ggaatgttgt atctgataat ctaaacaaag caataaaggt
      301 ttatgatttt aatattaaat acgaattcga tgagacttcg agaatattgg ctggaagatt
      361 tcttaacagt aagatttcca acataagtac aattgatgga gcgatggttg aaaaatcttt
      421 ttcatcatgg acctttggtt tggttggagg ttcgcgtcct gatttcagtg attttagtta
      481 taattcaaaa ttgtttgagt atggcggata tattagtaaa tctgatacat tcgggacagc
      541 gattatggaa aacacaatat ctgcatttaa tcaaactaat gattttaaaa ctgacagaag
      601 atttctatac tttcaacacg taaataattt gattaagaat acttttttat tcggatctgc
      661 agaagtggat tttttcaaaa tagccgatag tcttgctcag aatgatttta gtcttacagg
      721 tttattcttt tctgcaaggt atgctcctgt aagggagttc tcattaaacg tttcgtacga
      781 cgcaagaaga aacattattt attatgaaac ttataaatcc ttatcccaaa caatccttga
      841 aaacgaatta cgtcagggat ttagagctag agcgagcttt agaccactaa ctaatttatt
      901 ttttagttta caatatggat atcgtaatag gaaaggtgat ttaaaagcca gtacaaatta
      961 cggcgcgaac ctgggttatt ctttggttcc gcttattgaa tcatcagcaa atattaattt
     1021 taatagaata aacagcagtt atgtggatgg atatgtttat tcggctaatc tttctaaatc
     1081 tatttatgct ctaaattcag atttatctct tggatttaga aaaaccattt acaatttttc
     1141 taatggtgca agtcagcttg aagagaatgc tattttaatt gattattcaa taaatgcatt
     1201 tagagctttt tcaattaaca ttagttatga aggtgtattt gaatccaatc acacatatgg
     1261 cagaattctg tttgatataa caacaagatt ttgattcact caataaatac ttcctttcct
     1321 tataaactta ttatttatat ttttggctca aacaagagat aaaaataatg tttgaatacg
     1381 tcggctctat acatatgcac tcaacttttt cagatggcac aggagaagta tctgatattg
     1441 caaaatttgc tgatgagatt gggttggatt ttattatgct tactgatcat aacactctgc
     1501 gtgcattaca ggagggctat gaaaagtggt atggtaatac tttgctgtta gtcgggtgtg
     1561 aaataaatga taaagaaaac aaaaatcatt acttagcttt tggaattgat gaagcattca
     1621 cgaccagaac tccggcaaaa aaatatgttg caaaagttaa agagcttggc ggcattggat
     1681 ttttagctca tccgcacgaa aaacgaaaac acaaagaaca tcctgcttat ccctgggttg
     1741 aatgggacac tgaagatttt acaggtattg aaatctggaa tcacatgtct gagtgggttg
     1801 aaaatcttac tgaagaaaac aagtatagat catttcttca tcctttaaga acaatcattg
     1861 ccccgcctaa agaaacatta aaagtctggg atgaacttag tcagaaa
//
LOCUS       DASWBY010000593         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_738981, whole genome shotgun sequence.
ACCESSION   DASWBY010000593 DASWBY010000000
VERSION     DASWBY010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738981"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..346)
                     /locus_tag="VF870_10775"
     CDS             complement(14..346)
                     /locus_tag="VF870_10775"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX9252717.1"
                     /translation="MKIAKEFRWEMGHRLPEHFGLCKNIHGHSYKMIVEFEGALDKNQ
                     MVIDYYDVEKILNPVIEKLDHAFMVNKDDKIVLEFLEKMNSKKVVVEFNSTAENICSY
                     LLSEIKKC"
     gene            complement(346..987)
                     /locus_tag="VF870_10780"
     CDS             complement(346..987)
                     /locus_tag="VF870_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9252718.1"
                     /translation="MLKVNEIYYSVQGESTYAGLPCVFVRLTYCNLRCTYCDTEYAFY
                     DGKDFSISEIISEVKKYDCRLVEITGGEPLVQMEECLELMKQLCDDGFKVLIETGGSL
                     SIKNIDPRVSVIMDLKCPSSGMEKKNLYENIDYLKPADELKFVIGSREDYKWTKEIIL
                     KYDLKNKCQLLFSVVFGKLEPVQLVNWILEDKLNVRFQLQMHKFIWHPETKGV"
     gene            1117..1380
                     /gene="rpsT"
                     /locus_tag="VF870_10785"
     CDS             1117..1380
                     /gene="rpsT"
                     /locus_tag="VF870_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX9252719.1"
                     /translation="MAHHKSAKKRIRSSERKKAVNKMTDSKIKTVIKKTLATDKKEEL
                     EVLYKEAIAILDKGTSKGRLHKNNAARKKSKLTKHLNKVTAAK"
     gene            complement(1440..>1938)
                     /locus_tag="VF870_10790"
     CDS             complement(1440..>1938)
                     /locus_tag="VF870_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEX9252720.1"
                     /translation="DKNGIPLDIKIGKGAYIGDGVKLNYNVEIGRAAYLNGNVVFGKN
                     IKIWRIVHLSTFPNQTLTIEDNVKILWGDIIKGNIVIGENSTIESSVNMTGSDEFPLR
                     IGKNVLIKGSSYLFGSVVEDGVSIEHSVLIKKKVKAEKDKNENIKPVRFFIPETEGKE
                     VLSEI"
BASE COUNT          638 a          368 c          255 g          677 t
ORIGIN      
        1 tattttatgg gagttaacat tttttaattt cagataaaag gtaactgcaa atattttctg
       61 ctgttgaatt gaattccaca acaacttttt ttgaattcat cttttctaaa aattccagta
      121 caatcttatc atctttattt accataaaag catgatcaag tttttcaatt accggattta
      181 gaattttctc tacatcataa taatcaatta ccatctggtt tttatcgagt gcgccttcaa
      241 actcaacaat cattttatat gagtgcccgt gaatattttt gcacagacca aaatgttcag
      301 gaagtctgtg ccccatttcc cagcgaaatt cttttgcaat tttcattaca caccttttgt
      361 ttctggatgc caaataaact tatgcatctg aagttgaaat ctaacattta atttatcttc
      421 taaaatccag ttaactaatt gaactggttc aagtttacca aagactacag agaaaagcag
      481 ttggcattta tttttcaaat catattttaa aatgatttct tttgtccact tataatcctc
      541 cctgcttccg ataacaaact ttaactcatc agcgggtttt aagtaatcaa tattttcata
      601 taaatttttc ttttccatcc cgcttgatgg acattttaaa tccataatta cagatactct
      661 tggatcaata ttttttatgg agaggcttcc acctgtttcg atcaatactt taaatccatc
      721 atcacagagt tgtttcatca attctaaaca ttcttccatc tggactaaag gttcgccgcc
      781 ggtaatctca acaagtctac aatcgtactt ttttacttct gaaattatct cagagattga
      841 gaaatcttta ccatcgtaaa aagcatattc tgtatcgcaa taagtgcatc ttaagttgca
      901 ataagtaagc cgcacaaata cgcagggtaa tccggcataa gtgctttcac cctgtacaga
      961 ataatatatc tcattaacct tcagcataaa tagtgcgatt tttactcatt ttccaaataa
     1021 attgtgggca aagttactaa agctaagttt gacatcaaac gaggcattgc ctatatttgg
     1081 ctcacatttt ttaactaatt ataaggtttt ttgtaaatgg ctcatcataa atcggcaaaa
     1141 aagagaatac gttcatctga gagaaaaaaa gcagttaata aaatgactga ctctaaaatt
     1201 aaaactgtaa ttaagaaaac tttggctacg gataaaaagg aagaacttga agttttgtat
     1261 aaagaagcta tcgctatttt ggacaaagga acatctaaag gaagattgca caaaaataat
     1321 gcagccagaa aaaaatcaaa gttaacaaag catttaaata aagttacagc agctaaataa
     1381 tctgttagat tttaacaaaa aagctcttcg tttgaagagc ttttttagtt taaacagttc
     1441 taaatttcac tcaacacttc tttcccctca gtttcaggaa taaaaaatct cacaggtttt
     1501 atattttcat ttttatcctt ttccgcttta acttttttct ttatcaatac gctgtgctcg
     1561 attgaaacac catcttctac aactgatcca aacaaataac tagatccctt tatcaaaaca
     1621 ttttttccaa tgcgaagtgg aaattcatcg ctgcccgtca tattaacgct tgattcaatt
     1681 gttgagtttt ctccgattac aatgtttcct tttataatat cgccccaaag aattttaaca
     1741 ttatcttcaa tcgttaatgt ctgatttggg aaagtagaca ggtgaactat gcgccatatt
     1801 tttatattct ttccaaacac aacatttccg tttaggtatg ccgctcttcc aatctcaaca
     1861 ttgtagttaa gctttacacc atcaccaata taagcacctt taccaatttt gatatcaagc
     1921 ggaattccat ttttatcc
//
LOCUS       DASWBY010000594         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_58417, whole genome shotgun sequence.
ACCESSION   DASWBY010000594 DASWBY010000000
VERSION     DASWBY010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58417"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VF870_10795"
     CDS             <1..369
                     /locus_tag="VF870_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252721.1"
                     /translation="NWFVMVNAPHIHNQNWDAEIKVGRYNIIKKINEFLNTDIDKYIE
                     LEKLMSPVDIQNRTGSYLGSIYGIASNDKFAAFMRQSNKSKTIKDLYFCGGSAHPGGG
                     IPLVILSGKIVSDIIESEYQ"
     gene            369..1019
                     /locus_tag="VF870_10800"
     CDS             369..1019
                     /locus_tag="VF870_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9252722.1"
                     /translation="MIQAGHKKWAKFVFDIYLKRLFQKSFVDFRIVNDLPKLDESKSI
                     IVTPNHFSWWDGFFIYWLNKKVLNKKLYVMMLEEQLKRYWFFKKLGCYSIDLNDDKKM
                     IASLKYTMDLLLDSNNLVTIYPQGEIQAYDEKHIVLKEGIDFITKKSTVDFQILPIAF
                     KIHYTNEKLPIVYARFGKLLNSKEAFENQQLFKDEFINNLDQINSECSTSFFKSVL"
     gene            1023..2105
                     /locus_tag="VF870_10805"
     CDS             1023..2105
                     /locus_tag="VF870_10805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9252723.1"
                     /translation="MFIVLLISLSVFSVISIYNLLTAPVLNKESIHSSKQKLVSVLIP
                     ARDEEKNIEKCIKGMLEQDYENKEIIVLDDNSTDNTYKLASYFSSSNIKVLIGKELPT
                     DWLGKNWACHQLAQEAKGEYLLFIDADVELTPEVISSAIFELDKSNAALLSIFPTQII
                     KSFGEHLIVPLMNWLLLTFLPLKLVYASASKSFVAANGQFMLWKKDDYLKIGGHDKVK
                     NKIVEDMELARLAKQNRLKVKTMLGGKLVFCRMYESFYQAYNGFTKNFFAGFLLPPFL
                     FLIIIMFLLIAFLLPFLFLMQPFYSFILVALILITRISVSIVSNQNLFINILIHPAQM
                     LFMFWIGIVSIIKFKTKKIVWKQRKL"
     gene            2084..>2463
                     /locus_tag="VF870_10810"
     CDS             2084..>2463
                     /locus_tag="VF870_10810"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEX9252724.1"
                     /translation="MEAKKIIVSNKEIVFLYIIFFVGIIGHLYDPLQNLMLLLTPATL
                     LLTGVVVLLYSYKTSKNKFLLWVVLTYVITFLLEVKGVKTGLIFGEYKYGSTLGIKLF
                     DVPLIIGFNWVFVILGSISISRLIT"
BASE COUNT          851 a          327 c          403 g          882 t
ORIGIN      
        1 aattggtttg ttatggttaa tgcgccacat attcacaatc aaaattggga tgctgaaata
       61 aaagttggaa gatacaacat cataaaaaaa ataaatgaat ttctaaatac tgatatcgat
      121 aaatatattg aacttgaaaa acttatgtct ccggttgata tccaaaacag aaccggcagt
      181 tatctcggca gcatttacgg aatagcatct aatgataagt ttgcagcatt tatgagacaa
      241 tcaaacaaat cgaaaacaat aaaagatctt tatttttgcg gcggcagcgc acatccgggc
      301 ggcggaattc cacttgtaat tttatccggc aaaatagttt cggatataat tgagagtgaa
      361 tatcaataat gattcaggct ggtcataaaa aatgggctaa gtttgttttt gatatttatc
      421 ttaaaagatt atttcagaaa tcattcgttg attttagaat agttaatgat ctgccaaagc
      481 tcgatgaatc aaaaagtata atcgtaactc caaaccattt ttcatggtgg gatgggtttt
      541 ttatttattg gttaaataaa aaagtattaa ataaaaaact ttacgtaatg atgcttgaag
      601 aacaattaaa acgatactgg ttctttaaga agcttggctg ttattcgatt gatttaaatg
      661 atgataaaaa aatgattgca tctttaaagt acacaatgga tttactttta gattcaaata
      721 atcttgttac aatttatccg cagggcgaaa tacaagctta tgatgaaaaa catatcgtat
      781 taaaagaagg aatcgatttt attacaaaga aaagcacagt tgattttcag attttaccaa
      841 ttgctttcaa aatccattat actaatgaga aattgccaat tgtatatgcc cgatttggaa
      901 aattattaaa ttcaaaagag gctttcgaaa atcaacaatt atttaaagat gagtttataa
      961 ataatcttga tcagattaat tctgaatgct ccacatcatt ctttaagagt gttttataat
     1021 caatgtttat agtattacta atatctttga gcgtctttag tgttatctca atttataatt
     1081 tgttaacagc tcctgtatta aataaagagt caattcattc aagtaaacag aaacttgtct
     1141 cagttttaat tccggcaagg gatgaagaaa agaatattga aaaatgtatt aaaggtatgc
     1201 tggaacaaga ttatgagaac aaagaaatta ttgtgcttga tgataattct acagacaaca
     1261 cctataagct ggcttcatat ttcagttcga gtaatataaa agttttaata ggtaaagagc
     1321 ttcccacaga ttggcttggt aaaaattggg cttgtcatca gcttgcacaa gaagctaaag
     1381 gtgagtattt actctttata gatgctgatg ttgaactaac accggaagtt atttcatctg
     1441 ctatttttga attagacaaa tcaaacgcag cgttactttc tatttttcca actcagatta
     1501 taaaatcatt tggtgagcat ctaattgttc cgcttatgaa ttggcttctt ttaacttttc
     1561 ttccattaaa attagtttat gcttcagcaa gtaaatcttt cgttgctgca aacggacagt
     1621 ttatgctttg gaaaaaagat gattatttaa aaatcggcgg gcacgataaa gtaaaaaaca
     1681 aaattgttga agatatggag ctggcaaggt tagcgaagca gaatagatta aaggttaaaa
     1741 caatgctagg cggcaaatta gttttttgca ggatgtatga atctttttat caagcctata
     1801 atggatttac aaaaaatttt tttgctggat ttttgttacc tccattttta ttcttgatta
     1861 ttataatgtt tctgttgatt gcttttttac tgccgttttt atttttaatg cagccgtttt
     1921 attcatttat cttggtcgca ttaattttga taaccagaat atctgtttct attgtaagta
     1981 atcagaactt gtttattaat attttaattc atccagctca aatgttgttc atgttttgga
     2041 taggaatagt ttctataatt aagtttaaaa caaaaaagat agtatggaag caaagaaaat
     2101 tatagtttca aataaagaga tagttttcct ttatattatt ttctttgtag gaattatcgg
     2161 tcacctttat gatccattac aaaatctaat gcttctatta actcctgcaa cattattgct
     2221 tacaggagtt gtggttttat tatattcata caaaacttct aaaaataaat ttttactttg
     2281 ggttgtttta acttatgtta ttactttttt actcgaagtt aaaggagtta aaaccggact
     2341 aatattcggt gaatataaat atggttcaac tttaggaatt aaattatttg acgtaccatt
     2401 aataatcggg tttaactggg tttttgtaat acttggctca atttcaattt caagattgat
     2461 tac
//
LOCUS       DASWBY010000595         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_58424, whole genome shotgun sequence.
ACCESSION   DASWBY010000595 DASWBY010000000
VERSION     DASWBY010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58424"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VF870_10815"
     CDS             complement(<1..850)
                     /locus_tag="VF870_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX9252725.1"
                     /translation="MKKLFTNVSQIVTCSTNGKNFKRGKELSELEVLENHSIFCENGI
                     ILDILPNYSNSKIKFDEKVDLHGKVILPGLIDCHTHTAFAGSRANEFKEKIAGVGYEE
                     IAKKGGGINTTVTAVRNSSLIDLVELMKPRVQEFIAQGVTTLEIKSGYGLDFENEKKL
                     LHAIKVINETFAINIVSTFLGAHTFPPEFKDNHSGYVELIIDEMIPHIAKNNLAEFCD
                     GFCESTAFNSDEIDQIFIAASNSGLKLKLHTEQFNNVGGLDIALKHNAISVDHLEVLL
                     EKDFHKF"
     gene            complement(850..1407)
                     /locus_tag="VF870_10820"
     CDS             complement(850..1407)
                     /locus_tag="VF870_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX9252726.1"
                     /translation="MKKIIILGKPAIGVLISILLLAACSASTGSRYEKDEKEKSESVT
                     EDKNDNTNATTLKEDFDITPYKTEIIIPEKKKSGVVENQNVWFDYGSPEIETKTKTLI
                     GTTDGFRVLILTTDNLEEANQIKADVYFSQNSNETYIDFEPPFYKVKVGDFDNQKTAD
                     DLRFKLNQLGYKEAKVIKDKINIYK"
     gene            1580..3076
                     /gene="glgA"
                     /locus_tag="VF870_10825"
     CDS             1580..3076
                     /gene="glgA"
                     /locus_tag="VF870_10825"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX9252727.1"
                     /translation="MAAKKLKILFVTSEIVPFVKTGGLADVSSALPQMLSELGHEVRI
                     VIPKYGAVDDRKFKIHEIVRLKDLVVKIGDKDVTFSLKSCFLPGPRVRVQIYFLDNQE
                     YFGSRNSLYADLKTGKDFADNDERFILLSRSVMELIIKLGWIPDVIHLNDWQCGLVPA
                     YLKTIYKDQESFNKFKTLFTIHNLAYQGEFPAASFKKTGLPKELESVSKGIMHNGKLN
                     FMKSGLMFADVINTVSQTYANEIRTKEEYGEGLKDVLAKRKDSLYGIVNGIDKNVWNP
                     EKDKLIPANFSAKNIEEKLVNKKELAERFGLEYDEKVPIIGLISRLFDSKGFDLVQKA
                     FSDLMKLNVQMILLGTGDQKYHSFFDKMAFKYPKKFASYLGFNDELAHLIEAGADMFL
                     MPSKYEPCGLNQMYSLVYGTVPIVRETGGLADTVIKFNDKTEEGTGFVYKKHDAEDMV
                     KEIKKAIKVFEDKKTWQKIMRAGMKSDFSWDSSAKKYIELYKTMLSSD"
     gene            3083..>3631
                     /locus_tag="VF870_10830"
     CDS             3083..>3631
                     /locus_tag="VF870_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9252728.1"
                     /translation="MSKAVFLDRDGTLNLDPGYLGNPKDLKLFSDTGNVLAALKNNYH
                     FKLIVISNQSGVARGLITEEDVISVNEELNRRLLEFNVQIDAFYYCPFHPDFSNEEDC
                     FCRKPSPKMIFDSAKDFNIDLAKSYLIGDSVSDIKAGISADLKTILVKTGYGAESISI
                     LQKENNFPNFVAENLTEACNFII"
BASE COUNT         1228 a          549 c          610 g         1244 t
ORIGIN      
        1 aaaatttatg aaaatctttt tcaagtaata cttctaaatg atcaacgctt attgcgttgt
       61 gctttaaggc tatatccaga ccgccaacat tattaaactg ctcagtgtga agtttaagtt
      121 ttaatcctga atttgaagca gcaataaata tttgatcaat ttcatcagaa ttaaaagcag
      181 tcgattcaca aaatccatca caaaattctg caagattatt tttagcgata tgcggaatca
      241 tctcatcaat aattaattcg acatagccgg aatgattgtc tttaaattca ggcggaaaag
      301 tatgtgcccc aagaaaagtt gaaacaatat taattgcgaa agtttcatta ataactttga
      361 tggcatgcag cagtttcttc tcattctcaa aatccaaacc ataaccgctt tttatttcta
      421 aagtagttac accttgtgca ataaactcct gcactctggg tttcattaat tctacaagat
      481 caattagcga agaatttcta acagcagtta cagtggtatt tattccaccg cctttctttg
      541 ctatttcttc gtaaccaaca cctgcaattt tttccttaaa ttcatttgcc cgtgaacctg
      601 caaatgctgt gtgtgtgtgg caatcaatta atccaggcag aatgactttg ccgtgcaagt
      661 ctactttttc atcaaatttt attttggaat ttgaataatt cggtaagatg tcaagtatga
      721 ttccgttttc acaaaaaatg gaatggtttt ctaaaacttc aagttcagat aattctttgc
      781 cccgcttaaa attctttcca tttgtactgc atgtaactat ctgtgataca ttggtaaaaa
      841 gctttttcat tatttgtaaa tattaatttt atctttaatg acttttgcct ctttatatcc
      901 gagctgattt aatttaaatc ttagatcatc ggcagttttc tgattatcaa aatcaccgac
      961 tttaacctta tagaatggtg gttcaaaatc aatataagtt tcattagagt tctggctaaa
     1021 ataaacatca gctttaatct gattcgcctc ttccagatta tcagtcgtaa ggattaaaac
     1081 tctaaaacca tcggtagtac cgatcaatgt ttttgttttc gtttcaattt ccggggaacc
     1141 ataatcgaac caaacgtttt gattttcaac aacaccactt ttcttctttt caggaatgat
     1201 tatttctgtt ttgtaaggtg taatgtcaaa atcttccttt aaagtagttg catttgtatt
     1261 gtcattttta tcttcagtaa cactttcaga tttctctttt tcatcctttt catatctgct
     1321 accagttgaa gctgaacagg cagctaaaag cagaattgag atcaaaacac ctattgccgg
     1381 ttttccgaga attattattt ttttcataac tattatttgt attgaattat ctcaaatata
     1441 acctttttta gcaaagctaa gaaatagcat aaattataag gtttcttaaa atcgaacctg
     1501 aatattatac gggtcaattt ttgagaacca ccgctttttt tcgtattttt gcagcatcat
     1561 aaataaaaga attaccaaaa tggctgcaaa aaaactcaaa atattatttg ttacatctga
     1621 aatagtacct tttgtaaaaa ccggaggtct tgcagacgtt tcttctgcat taccgcagat
     1681 gttatcagaa ttaggtcacg aagtaagaat tgtgattccg aaatacggcg ctgttgatga
     1741 tagaaaattt aaaattcatg aaatcgttag attaaaagat ttagtagtca aaattggtga
     1801 taaagatgtt acattttctc taaaatcttg tttcctgccc ggaccaagag ttagagttca
     1861 gatttatttt ttggataatc aggaatattt tggcagccga aatagtttat atgctgatct
     1921 gaaaacagga aaagattttg cagataatga tgaaaggttt attctgctca gccgatcagt
     1981 aatggaatta attattaagc ttggctggat tccggatgtt attcatttaa atgactggca
     2041 atgcggatta gtgccggctt accttaaaac tatttataaa gatcaggaat cctttaacaa
     2101 atttaaaact ttgtttacaa ttcataattt agcttaccag ggtgaatttc cagccgcatc
     2161 atttaagaaa acaggattac caaaagaact tgaatcagtt tctaagggta ttatgcacaa
     2221 tggtaaattg aattttatga aaagtggatt aatgtttgcc gatgttataa atactgtcag
     2281 tcaaacttat gcaaacgaaa ttagaactaa agaagaatat ggtgaaggtt taaaagacgt
     2341 tttagcaaaa agaaaagatt ctttgtatgg aattgtaaat ggaattgata aaaatgtttg
     2401 gaatccggaa aaagacaaac tgatccctgc aaatttttca gcaaaaaata ttgaagaaaa
     2461 attagttaat aaaaaagagc tggctgaaag atttggatta gaatatgatg aaaaggttcc
     2521 aatcattggt ctgatctcaa gattatttga ttctaaagga tttgatttag tacaaaaggc
     2581 atttagtgat ctgatgaaat taaatgttca gatgatatta cttggtacag gcgatcaaaa
     2641 atatcattca ttttttgata agatggcttt taagtatccc aaaaaatttg caagctatct
     2701 tggttttaat gatgaacttg cccatttgat tgaagctggt gctgatatgt tcttaatgcc
     2761 atccaaatac gaaccttgcg gattgaatca gatgtacagc cttgtatatg gcaccgttcc
     2821 gatagttaga gaaaccggcg gattagctga tactgtaatt aagtttaatg ataaaacaga
     2881 agagggcacc ggttttgttt ataaaaaaca tgatgctgag gatatggtta aagaaattaa
     2941 aaaagcaatt aaagtatttg aagataaaaa aacctggcag aaaattatgc gtgcaggaat
     3001 gaaatccgat tttagttggg attcatctgc aaaaaaatat attgaacttt acaagacaat
     3061 gttatcatca gattagttga tgttgagtaa ggcagttttt ttagatcgtg atggtacatt
     3121 aaatttagat ccgggttact taggcaatcc aaaagattta aaattatttt ctgatacggg
     3181 aaatgtgctt gccgctctca aaaacaatta tcattttaaa ctcattgtaa tctcaaatca
     3241 atcaggtgtg gccagaggtt taattactga agaagacgtg atctctgtaa atgaagaatt
     3301 gaatagaagg ctcctggaat ttaatgtaca aattgatgca ttttattatt gcccatttca
     3361 tcccgatttt agcaatgagg aagattgttt ttgtagaaaa ccttcgccta aaatgatttt
     3421 tgattcagca aaagatttta atattgattt agcaaaatct tatttaattg gtgattctgt
     3481 ttcagatatt aaggcaggaa tatcagcaga tttaaaaaca atactagtaa aaacaggtta
     3541 tggtgcagaa agtatttcta tcttgcaaaa agaaaataat ttcccaaact ttgtagctga
     3601 aaatttaact gaagcctgta attttataat t
//
LOCUS       DASWBY010000596         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_58467, whole genome shotgun sequence.
ACCESSION   DASWBY010000596 DASWBY010000000
VERSION     DASWBY010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58467"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..779)
                     /locus_tag="VF870_10835"
     CDS             complement(6..779)
                     /locus_tag="VF870_10835"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX9252729.1"
                     /translation="MFSIENFDIKLNTEFIGRTFVYAEELSSTNTFLMDKKNGYNKSG
                     TVVLAEKQTSGKGRKDRVWYSAPDSNLTFSILLTKDKFFIKNSSLINFAASLSVAFSI
                     ENLYQLKTELKWPNDVLLDGKKTSGILIESSSQGNNIEKFVVGIGINVNQPSFQGTFN
                     YPPTSIRNELGRNVEREKLLAEILNNFEILLERLKKNREEVLNDWKSRCKMIGEKISI
                     IENDSEKFGIFYDIDEDGFLLLKTRDGIEKIHYGDVSLG"
     gene            1036..>2122
                     /gene="dnaK"
                     /locus_tag="VF870_10840"
     CDS             1036..>2122
                     /gene="dnaK"
                     /locus_tag="VF870_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX9252730.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNDPVVIPNSEGGRTTPSVVAFTK
                     SGERLVGQPAKRQAITNPNHTIFSIKRFMGRLINEVSKEKTEIPYEVVAGDNNTARVK
                     IGDRMYSPPEISAMVLQKMKKTAEDYLGQEVTEAVITVPAYFNDAQRQATKDAGEIAG
                     LVVRRIINEPTAAALAYGLDKKTSDHTVAVYDLGGGTFDISILALGDGVFEVKSTNGD
                     THLGGDDFDQRLIDFLADEFKKQEGIDLRKDAMALQRLKEAAEKAKIELSTSQSTDVN
                     LPFITATQDGPKHLNLNLSRAKFEQLIDDLVKKTKTPCEQAMKDAGVTPKDINEVILV
                     GGSTRIPMVQQLVKDLFQREPHKGVNPD"
BASE COUNT          720 a          354 c          370 g          678 t
ORIGIN      
        1 ctgttctaac cgagactgac atctccataa tgaatttttt caataccgtc tcttgttttt
       61 aagagtaaaa atccatcttc atctatatcg taaaatattc caaatttttc tgaatcattt
      121 tcaatgatag aaattttctc accaatcatt ttacatcttg acttccaatc attaagaact
      181 tcttctctgt ttttctttaa ccgttctaag agtatctcaa aattattaag tatttctgcc
      241 aatagttttt ctctctctac attcctgccg agttcatttc taatcgatgt gggcggataa
      301 ttaaatgtac cctgaaagga gggctggttt acatttattc ctataccgac tacaaatttt
      361 tcaatattat ttccctgtga acttgactcg attaaaatgc ctgatgtttt tttaccatcc
      421 agcagcacat cgttcggcca tttaagttca gtttttaatt ggtataaatt ttcgattgag
      481 aatgctactg agagtgaagc agcaaaatta attagactcg aatttttaat aaaaaattta
      541 tcctttgtca aaaggataga aaacgtaaga ttggaatcag gtgcactata ccaaactcga
      601 tcttttcttc ccttgccgct tgtttgtttt tccgcaagca ctactgtccc gcttttattg
      661 tagccattct ttttatccat taaaaaagta tttgtggatg atagttcttc agcgtaaaca
      721 aaagtgcgac cgataaattc tgtatttagc tttatatcaa agttttcgat actaaacaaa
      781 gtaactccaa atgatgatta taaaaatacg tttttttaat caaattgtca gttaggctga
      841 cgatttgcac ctgataaacg acataatgac atataaaact tattgacatt ttataaatgt
      901 aaaatgatct cttgttaagt cctttattta taagcactta aaataatttt cttagttggc
      961 actatcgttg taaactttct tttcagatta aataaaaatt aatgcgaatt aaataaatat
     1021 taaggagaat atataatggg taaaataata ggaatagacc tgggtacaac aaactcttgc
     1081 gttgcggtga tggaaggtaa cgatcctgtt gtaattccaa attctgaagg cggtagaaca
     1141 actccctctg ttgttgcttt tacaaaatca ggagaaagat tggttggaca acctgcaaaa
     1201 cgtcaggcaa taactaatcc aaaccataca attttttcaa tcaaaagatt tatgggaaga
     1261 ttaataaatg aggtttcaaa agaaaaaact gaaatacctt atgaagtagt tgccggtgat
     1321 aataatactg cacgagttaa gattggagac agaatgtact ctccacccga aattagtgct
     1381 atggttttgc agaagatgaa aaagactgca gaagattatc ttggtcagga agtaaccgaa
     1441 gcggttatta ctgtgcctgc ttactttaac gatgcacaaa gacaagcaac caaagatgcc
     1501 ggtgaaatag ctggtttggt tgtaagaaga attataaatg aacctacagc agccgcatta
     1561 gcatacggac ttgataaaaa aacaagtgac cacacagttg ctgtttatga tcttggtgga
     1621 ggaacttttg atatatctat actcgcgctt ggcgatggtg tgttcgaagt aaaatcgact
     1681 aacggcgata cacatttagg cggtgatgat tttgatcaga gactaattga ttttcttgca
     1741 gatgaattta aaaagcaaga agggattgat cttagaaaag atgcaatggc acttcagaga
     1801 ttaaaagaag ctgctgaaaa agcaaagatt gaattatcaa cttcacaatc gacagatgta
     1861 aatctgccct ttattacagc aacacaggat ggacctaaac atcttaactt aaatcttagt
     1921 cgtgctaagt ttgaacaatt aatcgatgat ttagtgaaaa aaacaaaaac accatgcgag
     1981 caagctatga aagatgccgg tgttacacca aaagacatca atgaggttat tcttgttggt
     2041 ggatctacaa gaataccgat ggttcagcaa ttagtaaaag atttatttca aagagaacct
     2101 cataaaggtg ttaatcctga tg
//
LOCUS       DASWBY010000597         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_620596, whole genome shotgun sequence.
ACCESSION   DASWBY010000597 DASWBY010000000
VERSION     DASWBY010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620596"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VF870_10845"
     CDS             complement(<1..692)
                     /locus_tag="VF870_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HEX9252731.1"
                     /translation="MFKKSAFLLTTLIVISSSLQAQTVVAKYAGEFMALGVGGRALGM
                     GGAFVAVANDVTAGYYNPAGLANLNYPQLALMHSEQFGNLVNYDYGAVAIPFQNDMSF
                     GLSVMRLGVDGIPDTRNALIDANGDGVIDINDDRLDYSRITEFSNQDWAFYLTFAKRQ
                     SEDFYWGINAKIVRRDLAEFSATGIGFDVGAYYTPLENFFLGANLQDATTTLVAWSTG
                     RNELVSPTLKVGA"
     gene            complement(821..1246)
                     /locus_tag="VF870_10850"
     CDS             complement(821..1246)
                     /locus_tag="VF870_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252732.1"
                     /translation="MKRFIFLIVAIVITLGCSEDPNDQQQKLIVKTDKNNYSINEIII
                     FSINNSSSTTAHLASCCTSVAFYIDRNENNNWVEHSNFGLPCLALCPGIDLTLNYFEI
                     LTDSIFLDESGTFRIRIPYTFNPDQNWTGEILSNSFTIE"
BASE COUNT          537 a          281 c          251 g          523 t
ORIGIN      
        1 gcaccaactt ttaaagtagg ggatacaagt tcatttctac cggtactcca ggcgactaaa
       61 gtcgttgttg catcctgaag gttagcgcca agaaaaaagt tttccagagg tgtgtaataa
      121 gcaccgacat caaagccgat accggttgca ctaaactctg ctaaatctct tctaactatc
      181 tttgcattaa ttccccagta aaaatcttca ctttgtcttt ttgcaaaagt taaataaaat
      241 gcccaatcct ggttactgaa ttcagtaatt ctgctgtaat caagtctatc atcattaata
      301 tcaattaccc catcgccatt tgcatcaata agagcatttc gtgtatcagg aattccatca
      361 acacctaacc tcattacact taatccaaaa ctcatatcat tttgaaaagg tatagcaaca
      421 gcgccataat cataattaac taagttgcca aactgctcag agtgcattaa agcaagctgt
      481 ggatagttaa ggtttgcaag tcctgcggga ttgtagtaac ctgcagtaac atcattggca
      541 acagcaacaa aagcgccacc cattccaagt gctcggccgc caacaccaag agccataaac
      601 tcaccagcgt attttgcaac taccgtttgt gcctgtagtg aggaactgat aactattaga
      661 gttgttaata gaaaagccga ttttttgaac ataaattgta actcctgtta gcaaaggata
      721 taaacttgat ggagaaaatt gagaatttga tatataaagt caaggctaaa ttaattagaa
      781 aatttatttg ttttgatatt tcttatagtt aatcagataa ttattcaatt gtgaaagaat
      841 tacttagtat ttcaccagtc cagttttggt ccggattaaa ggtataaggg attctgattc
      901 taaatgttcc gctttcatca agaaaaatgc tgtccgttaa tatttcaaaa tagttaagag
      961 ttagatctat tcccggacat aatgcaaggc aaggtaatcc aaaattactg tgttcaaccc
     1021 agttgttgtt ttcatttctg tctatgtaga atgcaacaga agtacagcag cttgcaagat
     1081 gcgcggtagt agaactagaa ttgtttatac taaagattat aatttcatta attgaataat
     1141 tgtttttatc agtttttacg atgagttttt gttgttgatc gtttggatct tctgagcagc
     1201 caagcgttat tacgatagca actataagga aaataaatct tttcatatta tactctctta
     1261 taaaaataac atcatgtctt ttataaaata aagcatatat tataatttta atagacaaga
     1321 tttttagctt gagaaatctt tttgtttaac ataaaattaa aatttgatta agcaagttca
     1381 aactaattgc aaaccaatac ggctaataat taaaatattt gtccggtttt gtttcttcaa
     1441 ttcagattcc aacaagtctc atagctaaaa atattacaag tccaattgta gcgcctaaat
     1501 aaatccctct gatatatttt cgtccgtctt tatctttaga tttcaataat cgtaatgatg
     1561 aataaacaat tgatacatcc attatgccga tt
//
LOCUS       DASWBY010000598         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_702864, whole genome shotgun sequence.
ACCESSION   DASWBY010000598 DASWBY010000000
VERSION     DASWBY010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702864"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VF870_10855"
     CDS             <1..491
                     /locus_tag="VF870_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9252733.1"
                     /translation="TTVKNGIESDLLKDLLAKGIIFDFADKYYYQNVNSDFNKIKDDK
                     ILSEFKKYLLDSDYKYESDVEKKINEIVKDLNEKHTTDDLSKTLEKLNLEVKQIFDVE
                     FAESTDEILSELKIELAHRYLGNDAEIKEALKYDKQFNAALNLFNDDATYSKLLSSEK
                     PK"
     gene            609..815
                     /locus_tag="VF870_10860"
     CDS             609..815
                     /locus_tag="VF870_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252734.1"
                     /translation="MRSHSRNVKEKIINKISFHKYYRCKNCGWRGLLATVRITSTSIS
                     VMFLYLLMIFGVGLITYQILKRLL"
     gene            1202..1774
                     /locus_tag="VF870_10865"
     CDS             1202..1774
                     /locus_tag="VF870_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9252735.1"
                     /translation="MKKSFLDYIFRNRLPVTLFVVVASFTLTFFISRAERDGVGYTPD
                     QPIAYSHKLHAGDMKIDCQYCHVGVEKSRHAMVPAVATCMNCHTVARKDKPEIIKLTE
                     YYRDGKPLRWKRIHKVPDYAYFNHSVHVNKGIDCTACHGQITQMEKVGQMNSFTMGSC
                     LNCHRNAHDKLPELKDKIKPGPEYCSACHR"
     gene            1807..1983
                     /locus_tag="VF870_10870"
     CDS             1807..1983
                     /locus_tag="VF870_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252736.1"
                     /translation="MNRKKFFTSISLGAVGFAVYNSFPMKFFSKPSAFNKNKIEVKVN
                     PLAVSRKKVGGKNV"
     gene            1976..>2795
                     /locus_tag="VF870_10875"
     CDS             1976..>2795
                     /locus_tag="VF870_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEX9252737.1"
                     /translation="MSEFKPDQTDPLQNSTPDPNYWRSFEALYGDPKVLEAKNHEFKE
                     GVTDDFSPAGLSGLSRRKFLALLGASAALAGTACTDYRDKGEIVPYNIKPEEITVGKP
                     NYYASTCTACPNSCGILIKTREGRPIKVDGNPDHPVNKGKICAKGQATILNLYDPERL
                     KNPLKQTDGIFNSISWKTADNDIIKELSSIGDREIAIVTHSVISPTVLKVFENFKAKF
                     PSTKIYSYELFNDSVKNSAWRKTYGSGNYPLIKWNEAKVILALESDFLGTEGNKV"
BASE COUNT         1018 a          410 c          459 g          908 t
ORIGIN      
        1 atacaactgt aaaaaatgga atcgaaagtg acttattgaa agacctttta gcaaagggca
       61 ttatctttga ttttgctgat aagtattatt atcaaaatgt taattcagat ttcaataaaa
      121 ttaaagatga taagattcta tctgaattta aaaagtatct tcttgattct gattataaat
      181 atgaatcaga tgttgaaaaa aaaataaatg agatcgtaaa agatctgaat gaaaaacata
      241 ccactgatga tttaagtaaa acactcgaaa aattaaattt agaagttaaa cagatttttg
      301 atgttgaatt tgctgaaagt actgatgaga tattatcaga attaaaaatt gaacttgcac
      361 atagatattt gggaaatgat gctgaaataa aagaagcatt aaaatatgat aaacagttta
      421 atgctgcctt aaatcttttt aatgatgatg caacttattc taagttatta agtagtgaaa
      481 agcctaaata accttctatg ggattataat atttaatgat taaatctttt tttcgcaaag
      541 ctaaagcatt ttataggatc aacccaacgt ttaatagttg cccgtcttgt aaaagttcag
      601 gcactataat gagatcacat agcagaaatg ttaaagaaaa aatcattaac aaaatttcat
      661 tccataaata ttatcgctgt aaaaattgtg gctggcgtgg gttattagca accgtcagga
      721 ttacttccac atcaatctct gtaatgttcc tgtacctgtt aatgattttt ggtgtcggat
      781 taattactta tcaaattctt aaaagacttt tatagccggg gcttttaatt ggacttctta
      841 tgtaataaat aactcttatg aaattttatc atatctagtc aaaaaactta acccctaatc
      901 cttaaaaatt cctttaatat aattcattca aaaatgcaaa atattgatag tactttaatt
      961 aaaaagttaa taaaaactta tttcttataa ttaaaagatt catttgattt aaaagcataa
     1021 attcataatt ttaaccgttc aaaaaagaaa taattagtta tgatcaaata gggaagtcct
     1081 gcttttttcc ctagttaatc cgcgcatttt taatctaaat aattgttata aattttacag
     1141 gatttctcct gttttatatg ttttttttaa tttttagccc taaataatta aagggattcg
     1201 gatgaaaaaa tcttttctgg attatatttt tagaaataga ttgcctgtta cattgtttgt
     1261 agttgttgca tcttttacac ttacattttt tatctccaga gcagaaagag atggggttgg
     1321 ttacacacca gatcaaccaa ttgcttattc gcataaactt catgccggtg atatgaaaat
     1381 cgattgtcag tactgccatg ttggtgtaga aaaatccaga cacgctatgg tacctgcagt
     1441 cgcaacttgt atgaattgtc acacagttgc tcgtaaggac aagccagaaa ttattaaact
     1501 tactgagtac tatcgggacg gtaaaccatt gcgatggaaa cggattcata aagttccgga
     1561 ttatgcctac ttcaatcatt cagttcacgt taacaaagga atcgattgta cggcttgcca
     1621 tgggcaaatc acccaaatgg aaaaagttgg acagatgaat tcttttacta tgggaagttg
     1681 tttaaactgt catagaaatg ctcacgataa attacctgag ctaaaggata aaattaaacc
     1741 cggaccagag tactgttcgg catgtcatag ataatgatat ttgaaaatca ataaaataaa
     1801 attggtatga atagaaaaaa attttttaca tcaatctctt tgggtgcagt tggatttgca
     1861 gtctataact catttcctat gaagttcttt tctaaacctt cagcctttaa caaaaataaa
     1921 attgaagtta aagtaaatcc tcttgcagta agcagaaaaa aagtaggtgg taagaatgtc
     1981 tgagtttaaa cctgaccaaa cagatccatt acaaaattca acgcctgatc ctaattactg
     2041 gagaagtttt gaagcacttt atggagaccc gaaagttctt gaagccaaaa atcatgaatt
     2101 taaagaaggt gtaacagatg atttttcacc agcaggttta tccggattat ctagaagaaa
     2161 atttcttgct ttactaggtg cttctgcagc tttagcggga acagcttgta cagattaccg
     2221 ggataaaggt gagatagtac cttacaacat caaacctgaa gaaataaccg taggcaaacc
     2281 aaattattat gcttcaactt gtacggcttg tcccaactcg tgtggaattt taattaaaac
     2341 gcgcgaaggc agaccaatta aagttgatgg aaatcctgat catcctgtaa acaaaggaaa
     2401 aatttgtgca aaaggacagg caactatatt aaatctttat gatcctgaaa gattaaaaaa
     2461 tccattaaag caaacagatg gtatttttaa ttctattagc tggaaaaccg cagataatga
     2521 tattattaaa gaattgtcgt ctattggtga tagagaaatt gcaattgtaa cacattcggt
     2581 tatatcacca actgtgctaa aagtatttga aaattttaaa gcaaaatttc cctcgacaaa
     2641 aatttattca tatgaattat ttaatgattc agttaaaaat tctgcctgga gaaaaaccta
     2701 tggttcgggc aattatccac taattaagtg gaatgaagca aaagtaattt tagctctcga
     2761 atcagatttt cttggaactg aaggtaataa ggttg
//
LOCUS       DASWBY010000599         8702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_174808, whole genome shotgun sequence.
ACCESSION   DASWBY010000599 DASWBY010000000
VERSION     DASWBY010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8702
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174808"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VF870_10880"
     CDS             <1..513
                     /locus_tag="VF870_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252738.1"
                     /translation="KSFVAANGQFMLWRKADYFKLGEHQVVKGKVVEDMELARLAKQN
                     GFKVKTLLGGNLVYCRMYNSFNEAYNGYQKNFYAGFSINPGFFIIIILFLLVVFLSPI
                     LIFINNIYYFIPLILVIIIRTAVSIKSKQNLLINIFLHLFQMLLMFWIGIVSVTKFKT
                     KQLVWKQRKL"
     gene            492..1136
                     /locus_tag="VF870_10885"
     CDS             492..1136
                     /locus_tag="VF870_10885"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEX9252739.1"
                     /translation="MEAKKIIVSNKEIIFLYVIFFVGIIGHLYDSLQNLMLLLTPATL
                     LLTGVIVLLYSYKSSKNKFLVWVILTYVITFLLEVVGVKTGLIFGEYNYGSTLGIKLF
                     NVPLIIGFNWVFVILGSISISRLITSNLILSAMISALIAFIFDLILEPIAIRLDYWTW
                     NGGIIPLQNYFAWFIIALISSFGFNYLKVTVNSKISLHYLLVQFVFFTMLLIFY"
     gene            1147..1818
                     /locus_tag="VF870_10890"
     CDS             1147..1818
                     /locus_tag="VF870_10890"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX9252740.1"
                     /translation="MGIIIALIIITIWSSHLIYSLLFVSVDFTNPIFYLHVLLQGYFY
                     TGLFITAHDAMHGTVSKNRFINKTIGWISTLLFAGLSYNKLIKNHFKHHKNPGEEGDP
                     DFYVKSQNFFIWWGSFLWRYTTIIQLIIMAIAFNVLKIWFDDNSIIMFWMVPAFLGTF
                     QLFFFGTYLPHKYPHTENMQPHKARTQKRNHLWAMLSCYFFGYHYEHHESPRTPWWKL
                     YKMKN"
     gene            complement(1829..3346)
                     /locus_tag="VF870_10895"
     CDS             complement(1829..3346)
                     /locus_tag="VF870_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9252741.1"
                     /translation="MVTTDFLKYVSLMYDKVRFPVQVAEQSGKIIYINEAFTILWGYH
                     LDELAEYSIFNDSVLKENNAVEKIMEVLNGKTYTAIDYYEDSLLRSRDYAIPILRTNI
                     FIIKDEDEPYIVMYHEDQTEMLLTEEEVKKARDASYESDRLKNTFLNVLSHELRTPLN
                     IILGYSSLIKENLADKIGPEDKIYLDNLHSGSERLFKSITQMLEFAQIEAGNYNLNTE
                     TTDLISIIQNCLPEHQKHAKEKSLELRTNFSHKKIYVDVDVQCVESALNNLISNAVKF
                     THHGYVEVEVSILQEKGLAICKVRDTGIGISTKYLDHLYQPFSQEDLNVGRNYEGIGL
                     GLALAKRYIEKLGGSLLVDSIKGVGSTFTFTLPLNQSKAFLAVSDSDELRGVNKILMI
                     DNSGETFELVKAFLKNSFSLSNYSIREFKLEFVQDSSYSHFIFDVDKNYWQQGIFICK
                     DIKRHDPFKRPIIIVSSEYIEEKIKEFYQAGATKFLVKPFGKSDLTKIINETIES"
     gene            complement(3348..4655)
                     /locus_tag="VF870_10900"
     CDS             complement(3348..4655)
                     /locus_tag="VF870_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9252742.1"
                     /translation="MKFSEFKKNIDSGIKTSLDADLTSEKAKNLEVILNILNSINRSL
                     ILDDVLELVLKNAIRLTNSERGFIVLKSHSGKLEFKLGLDSNGKELPESLFQISNSVV
                     EDVFYNGQSRFIEGAQSDVSLESSKSILRLDLQTILCSPLITDGNKIGVIYVDSKHLH
                     KIKEKETTSTYEILAGQAAAAIRNAQLYQSQLTANSALQEANTQLIQSERKALKAGID
                     SEIGQSLQGLVHLALLENESLLRMLERLKKENEDSVNGKESLLYDRLKLKSKVAIDSI
                     RGIQKYAQVLMETAIMNLNKDSGDLNRTIQSVIKYISPMKKYQFVTFKTQLNNIPLCN
                     YDSEQIQHLLVHLFTNSVNAQKDVTISIQSSADEKFVNIIVEDNGPGIPEDVKKDLFV
                     SYTPKRNSYGLFLCKSIVDRHNGEIKLLDSDTGTKIEISLPIN"
     gene            complement(4778..8434)
                     /locus_tag="VF870_10905"
     CDS             complement(4778..8434)
                     /locus_tag="VF870_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252743.1"
                     /translation="MKFIYTIVAFFVLYSISFSQDATKVALIHENSYDLIDAENQAGV
                     LYVSLFDFVNKLPVTISAEDKSAPVKLIFENHELYISSNNPFVTIIDLRDSSAKTLQL
                     SSPPYTKKSKIYISLISIVDLINKYWNKELIPLAANRIKIVNKVKESVKSEWVKSIQI
                     SSISIEYGPENVLFKIKSNGKIDNFYSFYRSQNLHLILWNTTISNDSALFLKSKEIVD
                     RVEINNGLQFLECKFFLNEKETITEVYEGKEDNELIIRISKRDFGDWYSKESEHFKII
                     YRDAHSHLVNHILSSAENSLAQLKKLFDYQPKEKIIINTYDVSDFGFGATTTIPENYI
                     RLEIEPLEPGYEMVPYSERFQWLLSHELVHIIVNDMASNFESSLRSVMGKVTPDKNQP
                     LTVFYSLLTNHNRYTPRWYQEAIAVFIETWFSGGYGRILGNFDEMYFRSLVDDNQKFA
                     SASEIENVTSHESILLENILYLYGTRFIANIANKYGTQKLYNWFSIKPDEFYPGLESK
                     FEKVYGIPLSDAWENFIADEKQFQNSNISLIKKYPLTEFRKLSDKPFGWVTHTTYDSK
                     NNSLIFGYHRKGELAEIQKFNLDNKESSYITTLPTPSLIQVAAVAYDESYKKLFYTTN
                     NNQLYRDVWEFDLNNNKEKLLFKDSRIGQLTISPSTHELWGIQHLSGKAILIRSKYPY
                     SEFQSLSVFDVGDEFSDLSINKKGNLLAAVLHRSSGQQSIIISDITGLEKGYPFQFRA
                     ITSSGSPENPSWSLDGKYLYWNAYTNGVSNIYRYDLQTEEIIALTNTIKGFFKPMEVS
                     KDSIIAMEFSLDGFTPVKFGISKADKLPAINYFGQKILDKSPELYELNLKPANKVIDK
                     NLFTKEESYSSLSDFSIKTFIPVVSGFQSRVVLGLFTQFNDPLLNHDLVIEAGVSPFK
                     ETTKDIKFHFRLKYSYHQKLTLSIEQNAPDFYDLFNTRKRGMLGGRYSVGYNHYWIFD
                     NPLKVKQMTELSIYRGIKFINDNLTEVRQPDFAILKSELEIRDLRKTIGSIDWESGDL
                     FKFSVLAYGSNPDDPQFSGQLMGEWDKYFLFLTAHNVLHLKAATGYHFINDNIPETMF
                     FFGGFGNREIENEPVKQFEKMFRFPGVPIYSITSDKFLKVMVENSLPPVRIPDLSFSS
                     IEFKNINVSIFSQGLISDSREIDKAVNLGAQLNIMFQHWYNLESTVSTGFAKAWWRSG
                     NDTEWFISWKLLKD"
BASE COUNT         2762 a         1449 c         1321 g         3170 t
ORIGIN      
        1 aaatcttttg ttgcggcaaa cgggcaattt atgctttgga gaaaagctga ttatttcaaa
       61 ttaggtgaac atcaagttgt taaaggcaaa gttgttgaag atatggagct agccagatta
      121 gcaaagcaga atgggtttaa agttaaaaca ttgttaggcg gaaatctggt ttattgcagg
      181 atgtataatt catttaacga agcatacaat ggttaccaga aaaattttta tgcgggattt
      241 tccattaacc caggtttctt cattataatt attctattct tattggttgt gtttctatca
      301 ccaattttaa tttttataaa taatatttac tacttcattc ctcttatttt ggttattatt
      361 attcggacag cagtttcaat taaaagtaaa caaaatcttt taataaatat ttttcttcat
      421 ttatttcaaa tgttattaat gttttggata ggaatagttt ctgttacaaa atttaaaact
      481 aaacaattgg tatggaagca aagaaaatta tagtttcaaa taaagagatc attttccttt
      541 atgtcatttt ctttgtagga attatcggtc atctttacga ttctttacaa aatctaatgc
      601 ttttattaac tcctgcaaca ttattgctta caggcgttat tgttttatta tattcataca
      661 aatcttctaa aaataaattt ttggtttggg ttattttaac ttacgttatt acctttttac
      721 ttgaagttgt tggagttaaa accggactaa tattcggtga atacaattat ggttcgactt
      781 taggaattaa attatttaat gtgcccttaa taatcggatt taactgggtc tttgtaatac
      841 ttggctcaat ttcaatttca agattgatta ctagtaattt aattttatct gcaatgattt
      901 cggctttaat agcttttata tttgatctta tacttgaacc tattgcaatc cggctggatt
      961 actggacatg gaacgggggt ataattccat tacaaaatta ttttgcctgg tttataattg
     1021 ctcttatttc atcattcgga tttaattatt taaaggttac tgtaaattca aaaatatcat
     1081 tacactatct tttggttcag tttgtatttt ttacaatgtt attaatattt tactaatgag
     1141 attgatatgg gcatcattat agcactaata ataattacta tttggtcatc acatctaatt
     1201 tattcactat tatttgtgag tgttgatttt actaatccga ttttttactt acatgtttta
     1261 ttgcaaggat atttttatac tggattattt atcactgcgc acgacgcaat gcacggcaca
     1321 gtgtctaaaa acagatttat taataaaact ataggttgga tatcaacttt gctttttgcc
     1381 ggattatcat ataataagtt gatcaaaaat cattttaagc atcataaaaa cccgggggaa
     1441 gaaggtgatc ctgattttta tgtaaaatcc cagaactttt ttatttggtg gggtagtttt
     1501 ctttggcgtt atacaacaat tattcagtta attattatgg ctattgcgtt taatgtgtta
     1561 aaaatatggt ttgatgataa ttcaataatc atgttttgga tggtgccggc ttttcttgga
     1621 acatttcagt tgtttttctt tggcacatat ttgccgcata aatatccgca cacagaaaat
     1681 atgcagccac ataaagcccg cacacaaaaa cgtaatcatc tttgggcaat gttatcctgc
     1741 tatttttttg gatatcatta tgaacatcat gaatcgccaa gaactccgtg gtggaaatta
     1801 tataaaatga aaaactagtt acgcgggatt aagattcgat tgtttcgtta atgatctttg
     1861 taagatctga tttaccgaat ggcttaacta aaaactttgt agctccggcc tgataaaatt
     1921 ctttaatctt ttcttctata tactcgcttg agacaataat gattggtctt ttaaatggat
     1981 cgtgccgctt aatatcctta caaataaata ttccctgctg ccaataattc ttatcaacat
     2041 caaaaataaa atgactataa gagctgtctt gaacaaactc aagtttaaat tctctaatac
     2101 tataatttga aagtgaaaat gaatttttta ggaatgcttt gacaagctca aaagtttcac
     2161 ctgaattatc aatcataagt attttattaa cgcctcgtaa ctcatcggaa tcgctaacag
     2221 ctaaaaaagc tttagactga tttaaaggaa gtgtaaatgt aaatgttgac ccaacacctt
     2281 ttatgctatc aacaagcagt gaaccaccta atttttctat atatctttta gctaacgcaa
     2341 gcccaagtcc tattccctca tagtttcttc ccacatttag atcttcctga ctaaacggct
     2401 gataaagatg atccaaatat tttgtggaga ttccaattcc tgtatcccga actttacaga
     2461 tggctaatcc tttttcctgt aaaatactaa cttctacttc cacgtaacca tgatgagtga
     2521 attttacagc attgctaatt aaattattta atgcgctttc tacacattga acatctacat
     2581 caacataaat ctttttatga gaaaagtttg ttctcaattc aagggatttt tcttttgcat
     2641 gcttttgatg ttcgggtaag caattttgaa tgatgctgat aagatcagtc gtttcagtat
     2701 ttaaattata attcccggct tcaatctgcg caaattctag catctgcgta atgcttttaa
     2761 ataatctttc acttccgcta tgaaggttat ccaaataaat tttgtcctca ggaccaattt
     2821 tatctgccag gttttcttta attaaggatg aatagcctag aataatattt aatggtgtgc
     2881 ggagttcatg agataaaaca tttaagaatg tgttttttaa tcgatcagat tcataactgg
     2941 catcgcgtgc ttttttaact tcttcttcag ttaaaagcat ctctgtctga tcttcatggt
     3001 acattacaat ataaggctcg tcttcatctt taataataaa aatatttgtt cttaaaatcg
     3061 ggattgcata atcgcgggaa cgtaatagtg aatcttcata ataatctatt gcagtatagg
     3121 ttttgccgtt tagaacttcc attatttttt ctacagcatt attttcttta agcacactat
     3181 cattaaatat ggagtactcc gcaagttcat ctaggtgata accccacaga attgtgaagg
     3241 cttcattaat gtaaattatt tttccgcttt gctcagctac ctgaactgga aagcggacct
     3301 tgtcatacat taaactaacg tattttaaaa aatcagttgt taccatttta attaataggt
     3361 aatgaaattt ctattttagt tcctgtgtct gaatctagta actttatttc accattatgt
     3421 ctatcaacaa tacttttaca taaaaatagg ccataactat ttcgctttgg tgtgtaagat
     3481 acaaacaaat cttttttaac atcttctgga attccagggc cattgtcttc tacaataata
     3541 ttaacaaatt tttcatcggc tgaagactga atactaatcg taacatcctt ctgggcattt
     3601 acagaatttg taaacaggtg cacaagtaaa tgctgtattt gttcagaatc ataattacaa
     3661 aggggaatat tattaagctg agtcttgaat gtcacgaact ggtatttctt cattggtgag
     3721 atatatttaa taactgattg tatggtcctg tttaaatctc cgctgtcttt attaagattc
     3781 attatagctg tttccattag aacctgagca tacttttgga tgcctctaat gctatcgatc
     3841 gctacttttg attttaattt caatcgatca tataatagtg attctttccc gtttactgaa
     3901 tcttcatttt ctttttttaa tctctccaac attctgagca gactttcgtt ttcgagtaaa
     3961 gctaaatgca caagaccttg taatgattgt cctatctcag aatcaatacc tgcttttaat
     4021 gctttacgct cagattgtat aagctgagta tttgcttcct gcaatgcaga attagcagta
     4081 agctggcttt gatataattg tgcattacgg attgctgctg ctgcctgtcc ggctaatatt
     4141 tcgtaagtac tggttgtttc tttttcttta atcttatgca gatgcttgct gtctacataa
     4201 ataacaccaa tcttatttcc atcagttatt aaaggtgagc aaagaatagt ttgaagatcc
     4261 aatcgaagta tacttttgct tgattctaga gaaacatcgc tttgtgcacc ttctataaat
     4321 cttgattgcc cattgtagaa aacatcttca acaacggagt tactaatttg gaaaagactt
     4381 tccggaagtt cttttccgtt tgaatccaat ccgagtttaa actcaagttt accagaatga
     4441 ctttttaaaa caataaatcc gcgttcagaa tttgttaaac gtatcgcatt cttcaaaacc
     4501 agttcaagaa catcatcaag aataagggag cggttaatgc tgtttaggat attcaaaatt
     4561 acttcaagat ttttggcttt ctctgaagta agatcagcat ccaggctggt ttttattccg
     4621 ctatctatgt ttttcttgaa ttctgaaaat ttcattttat gttaagtgtt ctcagataat
     4681 tattaagcaa gataattgtg caataattgt accgctttac agactaaata ttaacgaata
     4741 aactaagcaa ttatgtaaat aatacttaat aaaattacta atctttgaga agtttccagg
     4801 aaataaacca ttctgtgtca ttcccgcttc tccaccatgc cttcgcaaat cctgttgaaa
     4861 ctgtggattc cagattatac caatgctgaa acataatatt taattgtgcg cccaggttaa
     4921 cagctttatc aatttcacgt gaatcggaaa taagtccctg agaaaaaatt gatacattta
     4981 tgttcttaaa ttcaatgctg ctaaaggata aatcaggaat tctaactggg gggagagagt
     5041 tttctaccat cacctttaga aatttatctg aggtaatact gtagattgga acgccgggaa
     5101 aacgaaacat tttttcaaac tgcttaacag gttcgttttc aatttctctg tttccaaatc
     5161 cgccgaagaa aaacattgtt tctggaatat tatcatttat aaaatgatat cctgttgctg
     5221 cctttaaatg cagaacatta tgtgctgtta agaaaagaaa atatttgtcc cattcaccca
     5281 tcaactgtcc ggaaaattgt ggatcatcgg gattcgatcc atatgcgaga acagaaaatt
     5341 taaataaatc tcccgattcc caatcaatgc tgccaatcgt ttttctaaga tctcttattt
     5401 ctagttctga ttttagaatt gcaaaatcag gctgtctaac ctctgtaagg ttatcattaa
     5461 taaattttat accacggtaa atggaaagct ccgtcatctg tttaactttt aaaggattat
     5521 caaaaatcca ataatgatta tacccaaccg aatatcttcc accaagcatc cctctttttc
     5581 ttgtattaaa taaatcataa aaatcgggag cgttttgttc tatacttaat gtaagttttt
     5641 gatgataact atatttcaaa cggaaatgaa atttaatatc ctttgttgtt tctttgaacg
     5701 gcgagactcc tgcttcaata actaaatcat gattaagaag cggatcatta aattgtgtaa
     5761 acaggccaag cacaacacgt gattgaaatc ctgaaacaac tggaataaat gttttaattg
     5821 aaaaatcact taaagaagaa taagattctt ccttagtaaa aagattttta tcaattacct
     5881 tattcgcagg ttttaaattc aactcatata attccggtga tttatctaat attttttgtc
     5941 caaaataatt tatcgctggc agcttgtctg ctttagaaat tccaaactta acaggagtaa
     6001 atccatctaa actgaattcc attgctatta tcgaatcctt tgaaacctcc ataggtttga
     6061 agaagccctt tattgtattt gttaatgcaa taatttcttc ggtttgcaaa tcgtatctgt
     6121 aaatgtttga aacaccattt gtgtaagcat tccagtataa atattttcca tctaaagacc
     6181 atgatggatt ttccggcgag ccgcttgaag taatcgctct aaattgaaaa ggatatcctt
     6241 tctctaaacc agttatatct gaaataatta ttgattgctg cccacttgat ctatgtaaga
     6301 cagcagctag taaatttcct ttcttgttga tagataaatc agaaaattca tcaccaacat
     6361 caaaaacaga aagtgattga aattccgaat aaggatattt tgatcttata agtattgctt
     6421 taccgctcaa atgttgaatc ccccataact cgtgagttga gggagaaata gttaattgcc
     6481 cgattcttga atctttgaag agtaattttt ctttattgtt atttagatcg aactcccaaa
     6541 catctctgta aagctggtta ttgttggtgg tgtagaataa ctttttgtat gattcatcat
     6601 aagccactgc agcaacttgt ataagactcg gtgtaggaag tgttgttata taactcgatt
     6661 ctttattatc cagattaaac ttttggatct ctgctaattc accttttcga tgatatccaa
     6721 atattagtga attatttttc gaatcataag ttgtatgtgt aacccagcca aatggcttat
     6781 cggaaagttt tcgaaattcc gttagtggat attttttaat aagtgaaata ttagaatttt
     6841 gaaattgctt ctcatctgca ataaaatttt cccacgcatc actaagtggt attccataaa
     6901 ctttttcaaa ctttgattct aacccgggat aaaattcatc gggtttaatc gagaaccagt
     6961 tatataactt ttgtgttccg tatttatttg caatgttagc aataaatctt gtaccatata
     7021 aataaagtat attttccagc aatatacttt catgagaagt aacattttct atttcagagg
     7081 cacttgcaaa tttttgatta tcgtctacca aagatctaaa gtacatctca tcaaaattac
     7141 caaggattct accataacct ccgctaaacc aggtttctat aaaaacagcg attgcttcct
     7201 ggtaccatct tggagtgtat ctgttatggt tagttagcaa actataaaat actgttaatg
     7261 gttgattttt atccggagtt acttttccca ttacacttct taaagatgat tcgaaatttg
     7321 aagccatatc attgacgatg atgtgaacta gttcatggct aagcagccat tgaaatcgtt
     7381 cagaatatgg aaccatttca tatcctggtt ctaatggttc aatctcaagt cggatataat
     7441 tttcgggaat agtcgtggta gccccaaaac caaaatcgct tacatcataa gtattaataa
     7501 taattttttc tttgggttga taatcaaaaa gtttttttaa ttgtgctaaa gaattttctg
     7561 ccgaagaaag tatatggttt actaaatgag aatgtgcatc acgataaata attttaaaat
     7621 gttcactctc tttgctatac caatctccaa aatctctttt acttattctt ataattaatt
     7681 cattatcctc tttcccttcg tagacctcgg taatcgtttc tttttcatta agaaaaaatt
     7741 tgcattctag aaattgtaaa ccattattaa tttctactct atcaacaatt tctttcgatt
     7801 tgagaaaaag tgcagaatca tttgatattg tggtattcca taaaattaag tgcaggtttt
     7861 gcgatcgata aaaactataa aagttatcaa ttttgccgtt tgatttaatt ttaaataaaa
     7921 cgttttcggg cccatactca atagaaatac tagaaatttg tatacttttt acccactctg
     7981 atttaactga ttcttttact ttatttacaa tctttattct atttgcagct aagggaatta
     8041 actctttatt ccaatactta tttatcaaat caacaatcga aattaatgaa atataaattt
     8101 tagacttttt agtatatgga ggacttgata actgtaacgt ttttgctgaa ctatctctta
     8161 aatcaattat agtgacaaac ggattatttg agctaatata tagttcatgg ttttcaaata
     8221 ttaatttaac tggtgcagat ttgtcttcag cagaaatggt tactggcagt ttatttacga
     8281 aatcaaaaag tgaaacatac aatacaccag cttgattttc agcgtcaatc agatcataac
     8341 tattttcatg aatcagtgct actttagttg catcttgaga gaatgatata gaatataata
     8401 caaagaaggc aacaatagtg taaataaatt tcatgaaaat tattaagcga gatatttact
     8461 aatgatattt tttttatgct tcaatttaaa aaaaaagcct tcaaataatt gaaggcttaa
     8521 aaataaaatt tagtacaaaa gacagattat cgtgcccaac cgtatcttcc gcctaattcc
     8581 tcaagctgag atgatgatcg tgcccaaccg tatcttccgc ctaattcctc aagctgagat
     8641 gatgatcgtg cccaaccgta tcttccgcct aattcctcaa gctgagatga tgatcgtgcc
     8701 ca
//
LOCUS       DASWBY010000600        11689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_58944, whole genome shotgun sequence.
ACCESSION   DASWBY010000600 DASWBY010000000
VERSION     DASWBY010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11689
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58944"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /gene="scpA"
                     /locus_tag="VF870_10910"
     CDS             <1..1894
                     /gene="scpA"
                     /locus_tag="VF870_10910"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX9252744.1"
                     /translation="STAEESNAFYRRNLAQGQMGLSVAFDLATHRGYDSDHPRVVGDV
                     GKAGVAIDSIADMKILFDQIPLDKMSVSMTMNGAVLPVLAFYIVAAEEQGVKHEQLTG
                     TIQNDILKEYMVRNTYIYPPEVSMRIIADIFMFTAKNMPKFNSISISGYHMQEAGATA
                     DLEMAYTLADGLEYVRTGIKAGMDVDEFAPRLSFFWAQGMNYFMEVAKMRAARLIWAK
                     MIKQFNPKDPKSMSLRTHSQTSGWSLSEQDPFNNVVRTCIEAMAAALGHTQSLHTNSL
                     DEAIALPTDFSARIARNTQLYLQDETYITKVIDPWGGSYYVEALTDALLKKGWEHIIE
                     IESYGGMTKAIEAGIPKMRIEEASARRQARIDSGRETIVGINKYRLEKEDPIEILEVD
                     NTAVRLSQIKRLNEVKKNRNNEDVKKSLEALTKCAEIGEGNLLELSIVAARARATLGE
                     ISSAIEKVSGRYKAVTRTISGVYSSEYAKDDELFEQVRKMTDDFAKHEGRRPRIMIAK
                     MGQDGHDRGAKVVATAYADMGFDVDVGPLFQTPEETAQQAVENDVHVVGMSSLAAGHK
                     TLLPQLIAELKKLGREDIIVICGGVIPAQDYDFLYEHGTSAIFGPGTKIPEAGIKIME
                     LVKERF"
     gene            1965..2037
                     /locus_tag="VF870_10915"
     tRNA            1965..2037
                     /locus_tag="VF870_10915"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1998..2000,aa:Arg,seq:cct)
     gene            complement(2038..2814)
                     /locus_tag="VF870_10920"
     CDS             complement(2038..2814)
                     /locus_tag="VF870_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX9252745.1"
                     /translation="MVDKYDSFIFDLDGTVYRGDDIIPGADITINRLKESGKKIIFIS
                     NKTTGTIKDYYIFLKSFGLNIEESEILNSTFVLKNYLKDNYFHSVFYAIGEEIFIQEI
                     SQAGLNYSEDPMEIEILLVTLDRTLNYKKLETAANALEKGARFFAANIDDTCPVSGGE
                     VLDAGSTISALEKRTHRKLELNFGKPSKFMMDEVMKRLSSDKSKTLLIGDRLETDIAM
                     GNKFGIDTALVSTGVKHYANGNSEIKPTYYLNSVVDILRK"
     gene            complement(2819..4591)
                     /locus_tag="VF870_10925"
                     /pseudo
     CDS             complement(2819..4591)
                     /locus_tag="VF870_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
     gene            complement(4588..5502)
                     /locus_tag="VF870_10930"
     CDS             complement(4588..5502)
                     /locus_tag="VF870_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9252746.1"
                     /translation="MSPINLIKRRILVVGANGMLGQRVIEFYSGLNDVELLATSVEDK
                     FVFEGLEYIQSDISNRNEIKKVVKDFCPDFIINAAAFTNVDLSETERELAWIINVKGV
                     EYLSESARVLDSHLIHISTDYIFDGKSGPYLEIDIPNPLGYYARTKLASENVLKISGI
                     KYTILRTNVLYGTAKFSRPDFVKWVVDSLRANKPIRIVDDQINNPTFIDDLVQGINKT
                     IELRKEGIYNIGGNEFLSRYDFTIIIADFFNLDKSLISKIKTEELNQPARRPLKSGLI
                     TIKAQSELGYKPHTIIQTLALMKKELGL"
     gene            complement(5508..6371)
                     /locus_tag="VF870_10935"
     CDS             complement(5508..6371)
                     /locus_tag="VF870_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX9252747.1"
                     /translation="MQTKFIKMKLLVIGHSVLDFIKSDREQNISAGGIFYSISALNRL
                     KSAHEEIFLCSQFDDETYKYFKPEFEKVNDKFIQKVEKIPRVHLNLQKDRERHEAYEN
                     ITNNLSLNFSDLISFDGILINMITGFDITLDQLRQIRNNYSGLIFIDIHTLSRGLSAD
                     YKREFRLIPDFDNWAKCLDIIQVNQHELFTLSNKKSEMDIVEDILAIGVKIISVTKGD
                     LGAKIYYKNQNEIASYFVAAKKIINPNIIGCGDVFGASFFYSYIRNKNAMDSLTNAVA
                     NAEQFVENKFL"
     gene            6490..7617
                     /gene="bshA"
                     /locus_tag="VF870_10940"
     CDS             6490..7617
                     /gene="bshA"
                     /locus_tag="VF870_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEX9252748.1"
                     /translation="MKIGITCYPTYGGSGVIATELGKELALRGHEVHFISYALPFRLT
                     KYVENIFFHEVETSTYPLFEFPLYSLSLASKMIEIAEFENLDLLHVHYAIPHATSAYL
                     AKQIVKNKKLKITTTLHGTDITLVGLEPSFLPLVKFSIEQSDGITAVSRFLKEKTLTN
                     YSIEKEITVIPNFVDTDVFKKVSIDDCAFRKHIAPNGEKVLVHTSNFRPVKRVTDAIR
                     VFNIVQKEVPSKLLLVGDGPDRSECERLTRELNLTDCVKFLGKQEGLVEILSSSDVFL
                     IPSQSESFGLAALEAMACGLPVVSSSVGGLPELVKHNECGFIAEIGDIERMAKYTLDL
                     LTNDKKYEIFSQNARNRTLNNFDKSIVVPLYEQHYKNILEQ"
     gene            7619..8041
                     /locus_tag="VF870_10945"
     CDS             7619..8041
                     /locus_tag="VF870_10945"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEX9252749.1"
                     /translation="MIIYCAGPIRGNTTYQDNYSEIVRIVESMGHTALSEKSSKFSSS
                     IMLSDKQVYTRDIKWIDASKIVIAEVSGPSLGVGFEVSYALFVKKMPVLAVYHDQAGQ
                     ISAMITGCTDPKLQIKKYSNVDDLTSIIKKFILSNGGN"
     gene            8038..8466
                     /locus_tag="VF870_10950"
     CDS             8038..8466
                     /locus_tag="VF870_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252750.1"
                     /translation="MNEKLFIESWLNKIQTAGLKQFPLHFIDETRLDIISIPTKTLVI
                     GQEFFGAYEIITTDGESVYQASNYDEAKFIVYSSKERNGKSYLPKDRSMIKTLVDSYN
                     NYLDELLGQIKKDYKKNFQDGKNVHGVSNEIFKKLNLIRY"
     gene            8469..9902
                     /locus_tag="VF870_10955"
     CDS             8469..9902
                     /locus_tag="VF870_10955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX9252751.1"
                     /translation="MATEVSQKIYDYFSSLYGKEFADKYIQFIDQDSAQYIRVNTAKI
                     TREDLSQLLFEKYQIKTLADKHFEKVLKIIEGNDRIGKTFEHVLGFYYIQSLSSMMPP
                     LILNPTSEDIVLDLCGAPGSKSTQIAEIMNNRGALLINEVDNERIKSLVFNLERMNVI
                     NASVIHSKGEILSKVYDDYFTKVLVDAPCSGLGIIQKKGEVSNWWSLDHVDRLQHLQM
                     RLLIAAIKMAKVGAEIVYSTCTLSVEENELVIDKILEKYPVELLEIDLPIPSRPAFTE
                     YDGKQLNPELKKAIRILPWEIDSDGFFLVKMRKVNVTDSPEKDDVRETENRIVKSNHK
                     EIQQYINYISEHFGIDRKVLDDYKYIFRGKDIFFVIKDWDEEHIGLFNRIGLKLGTLD
                     KKDRITFNSQAAQVLEKYITKFIYELKNEDELKNYLTGWKIKEIDIPLGQYVVKYNNW
                     ILGTAVMTVEGLKSRFPRTKRTQKFEF"
     gene            complement(9907..11346)
                     /locus_tag="VF870_10960"
     CDS             complement(9907..11346)
                     /locus_tag="VF870_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9252752.1"
                     /translation="MIRQILFTIFLIAQISHAQGYGTLRFTNYANDRQSAFSLTFDDG
                     LLTHIENVGPILNQYGIKGTFYVLPPYLTETLPGIWRYGTWPGFQSLAAEGHEISSHT
                     MNHYDLTSLLWGSINDEGTLLYELCQSQLFIQQKIPAEKCISLNYPYTIHNSVVDSAA
                     RLFYENGRTLGQVPNDSSLSEEDWFGLKAKVVEFSEPRNSVADDLDELYSFLEWTQNS
                     INNHKWGMIIIHDVVPFDQLQDLLDQGIYEPVTNEWLTSLCDFLWARSSDKEVWVETV
                     GNITRYIKERDDAQYQIISSSNQLIQINVSDNLENAIFNYPLSAYVKVPNNWEYVRTE
                     QNGIVDTLTSIVTDSGNVVLAKVIPDNGILKLSPVTATNVENEITEIKDFELYQNYPN
                     PFNPSTKISWQSPIAGWQTLKVYDVLGNEVVTLLNEYRPAGTYEAEFNLANESIRASG
                     VYFYKLQVGDPSTGSGRGFVQTKKMILLR"
     gene            11532..>11689
                     /locus_tag="VF870_10965"
     CDS             11532..>11689
                     /locus_tag="VF870_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX9252753.1"
                     /translation="MLKPKLFTTLKDYTSKQFIADLSAGTIVGIVALPLAIAFGIASG
                     VTPEKGLIT"
BASE COUNT         3895 a         2058 c         1986 g         3750 t
ORIGIN      
        1 ttcaactgct gaagaaagta atgcttttta tcgcaggaat cttgcacaag gacaaatggg
       61 gctttctgtt gcattcgatc ttgcaactca ccgtggttac gattctgatc atccaagagt
      121 tgttggtgat gttggtaaag ccggagttgc tattgattca attgcagata tgaaaattct
      181 tttcgatcag atcccgcttg ataaaatgtc cgtctcaatg acgatgaacg gtgctgttct
      241 tccagtactt gcattttaca tcgttgcggc agaagaacaa ggagtaaagc acgaacagct
      301 tacgggaaca attcaaaatg atattttaaa ggaatatatg gttcgcaata cttacattta
      361 tccaccagaa gtttcgatga gaattattgc tgatattttt atgttcacag ctaagaacat
      421 gcctaagttt aatagcattt caatttcagg atatcatatg caggaagccg gtgctactgc
      481 agatcttgaa atggcataca ctcttgctga tggattggaa tatgttcgta ctggaattaa
      541 agcaggaatg gacgttgatg aatttgcccc acgtttatct ttcttctggg cacagggaat
      601 gaactatttt atggaagtag caaagatgcg cgcagcaaga ttgatctggg caaagatgat
      661 taaacagttc aatccaaagg atcctaaatc aatgtcgcta agaactcatt ctcaaacttc
      721 cggttggagt ctatctgaac aggatccgtt taacaatgtt gtaagaacct gcatcgaagc
      781 aatggcagca gcacttggtc atacacaatc acttcataca aattcattag atgaggcaat
      841 tgcattgcca acagattttt ctgcacgaat tgcccgcaac acgcagcttt atttacagga
      901 tgaaacttac attacaaaag taattgatcc gtggggagga tcttattatg ttgaagcatt
      961 aactgatgca cttcttaaaa aaggatggga acatattatt gaaattgaat cttatggtgg
     1021 aatgacaaaa gctattgaag caggaattcc aaagatgcga attgaagaag cttccgcaag
     1081 aaggcaggca agaattgatt caggcagaga aacaatagtt ggaataaata aatacagatt
     1141 ggaaaaagaa gatcctattg agattttaga agttgataat acagcggtaa gattatcaca
     1201 aataaaaaga ttgaacgaag taaagaaaaa caggaataat gaagatgtta aaaaatcact
     1261 tgaagcttta accaaatgtg cagaaatcgg agaaggaaat ttactggaac tttcgatcgt
     1321 tgcagcaaga gcacgtgcaa cacttggaga aatttcatca gcaattgaaa aagtaagcgg
     1381 aaggtacaaa gcggtgacaa gaacaatatc aggagtttac agcagcgaat atgcaaaaga
     1441 tgacgaactg tttgaacagg ttcgcaaaat gacagatgat tttgcaaaac atgaaggccg
     1501 ccgcccgaga ataatgattg caaaaatggg acaggatgga cacgatcgcg gagcaaaagt
     1561 tgtagcgact gcttacgccg atatgggatt tgatgttgat gttggtccgt tattccagac
     1621 tcccgaagaa actgcgcagc aagcagttga aaatgatgtt cacgttgttg gaatgagttc
     1681 tcttgctgcc ggacataaaa cacttttacc tcagctcata gcagaattaa aaaaacttgg
     1741 aagagaagat attattgtaa tttgcggcgg agtcattcct gcacaggatt atgattttct
     1801 ttacgagcat ggtacctcag caatctttgg tccgggcaca aaaattcccg aagcggggat
     1861 taagataatg gagttggtaa aagaaagatt ttaaattcac atcctaagat tacaaaagtg
     1921 attaagtggt gggatctctt taaatttaca aagcaattat ttctgccctc gtagctcagt
     1981 ggatagagca gaagtttcct aaacttttgg tcggaggttc gattcctctc gagggtacta
     2041 ttttcttaaa atatcaacaa ccgaatttaa atagtaggtt ggtttaattt ccgaatttcc
     2101 atttgcgtaa tgtttcactc ctgtagaaac caaagctgta tcaatcccaa acttgttacc
     2161 cattgcaata tcagtttcta atctatcgcc aataagaagt gttttagatt tgtctgagct
     2221 cagccgcttc ataacttcat ccatcataaa ctttgacggt ttaccaaaat ttaattcaag
     2281 ttttctgtga gtgcgttttt ccaatgcgga tattgttgat ccggcgtcca gcacttcccc
     2341 accgctgacc ggacaagtat catcaatatt tgcggcaaaa aatcttgcac ctttctctaa
     2401 agcattagcg gcagtttcta attttttata attaagagtg cggtcaagag taaccagaag
     2461 tatctcaatc tccataggat cttcggaata atttagaccg gcttgagaga tttcctgaat
     2521 aaatatctcc tcaccaatag cataaaatac tgaatgaaaa taattatcct ttaaataatt
     2581 ttttaataca aatgtagagt ttaagatttc cgattcttca atattcagcc caaatgattt
     2641 aagaaatatg taataatcct taattgttcc ggtagttttg ttggatataa aaattatttt
     2701 tttgccggat tccttcaggc gattaatggt tatatcagca ccggggataa tatcatcgcc
     2761 tcgataaaca gttccatcaa gatcaaaaat aaatgaatca tatttatcta ccataaaact
     2821 aatttactgt cctataaact ttaaaagatt taggggcaat tgatttaatg ttacttccgc
     2881 gattgtttgg atataattct agaacttcat aatctaaagg gagagtaatt tccgcttcac
     2941 ctaagttaaa tacacaaata atttttccat ttccgaaaac gctttcaaat gcaagggcgt
     3001 ttttgctgtt atcagaataa ataaatttaa atgtccctgt ttttagttct tcattattat
     3061 ttcggagatt aataataact ttgtaaaatt ttaataaaga ctcgttctgt tcaactgaat
     3121 atgaacccca gccggtttta aatcctgaat tctcctctat gacttcatta tcgtaaacca
     3181 ggtcgttcca caccattggt tttctatcat gcggatcatc ggcgccccac attccaacct
     3241 catcaccgta ataaatcata ggagcaccac gataaagtaa ttgaaatgct gcaattagtt
     3301 tttgtttttc aatatcatcg ctggttggct tgcctgggtt ataatttgga ttgttttcat
     3361 ttgcatcatg ctcgtatttg cgatcaggat tttttatcat tgatgaaaga cgttcagtat
     3421 catgacttga taacaaattt tgaagcacga ataaattttg ataaggataa gtctcatcaa
     3481 ttttttgtaa tctctgaata aactcatcaa ccgaaatttt atttttatca gcaataaaaa
     3541 aatcatttac tgcaaacgca aaattatagt tcatcaaagc atcaaaagcg ccatcatcag
     3601 aaataaaatc cggagaaagt tcccacaatt cgcccacaat tagtgattgc ttattgatcg
     3661 ttttaacaac attgcgccat tgcttccaaa atccaattgg cacctctctt gcaacatcaa
     3721 ggcgccagcc atcaattcca tctgaaggat caccatcacc atttggatcc atccatttgt
     3781 gtgttgagtg aaaaatatat tgtttcggtc catcagctaa atcatctttt gttctattaa
     3841 attctggtaa tgattttgcg ccccaccagc ctttgtaatt aaactcgttt tctgttgtac
     3901 ttggatcatc aaacgattct atcaaatacc aatccttgta cttggattgt tcctggtatt
     3961 taacaatgtc ttgaaatgcc cagaactgca cacctgtatg attaaaaacg ccatctataa
     4021 taattttcat cccgcgtttg tgaactccat caatcaactt taaaaataat ttatcagctg
     4081 aagtccactt ccaagtatca ggattatcgg gaatttcaga attcattatt tttcaatcgc
     4141 cttcgggatc gggtccaaag tttacatcga tatgatgata acttgatcca tcatatttat
     4201 gaagtgagac agcctcaaaa acaggattta gataaattgc tcctactcct agatctttta
     4261 aataatcaag tctgtctatt attccctgaa tgtcaccgcc ataccttctc tcataatagt
     4321 gatgatcatt ttttcctccc atttcttttt cccaacccga ctgggcaaac caattacttg
     4381 tccattttgt aacagtccaa ccttcgggag ttcttttatt attaataaat actttttctg
     4441 cttccggatc atttgttgtg tctccatttg caaatctttc cgggaagatt tgataccaca
     4501 caacaccttt tgcccattgc ggaacttctt ctttttgagg gaaggctgaa acagtgatta
     4561 caatcagcaa taaaaataat tttgctttca taagccaagt tcttttttca ttaatgctag
     4621 agtttgaata attgtatgtg gcttgtatcc aagttcgctt tgagctttaa tagttataag
     4681 tcctgattta agtggtcttc gtgcaggttg atttagttct tcagttttta ttttactaat
     4741 caatgattta tcaagattaa agaaatctgc aataataatt gtaaaatcat aacgcgaaag
     4801 aaattcatta ccgccaatat tgtagatacc ttctttacga agctcaattg ttttgtttat
     4861 tccctgaacc aggtcatcaa taaaagttgg attgtttatc tgatcatcaa ctattcgtat
     4921 tggtttatta gcccttaaag aatcaacaac ccattttaca aaatccgggc ggctgaattt
     4981 tgctgtgccg tataaaacat ttgttcttaa aatagtgtat ttgattccgc ttatttttag
     5041 aacattttca ctcgctagtt ttgtccttgc ataataacca aggggatttg gaatatcgat
     5101 ttcaagatat ggtccgcttt taccatcgaa tatataatct gtagaaatat gaatgagatg
     5161 tgaatctaaa acccttgcgg attcagaaag atactcaaca cctttaacat ttattatcca
     5221 ggcaagttct ctttcggttt cacttaaatc tacatttgta aaagcggcgg catttattat
     5281 aaaatccgga caaaaatctt ttacaacttt tttaatttca ttacggtttg aaatatcact
     5341 ctgaatatat tcaagtcctt caaacacaaa tttatcttct acggatgtgg ctaataactc
     5401 aacatcatta agtcctgaat aaaactcaat tacacgctgc ccgagcattc catttgcacc
     5461 aacaacaagt attcttcttt ttataagatt aataggcgac ataataatta caaaaatttg
     5521 ttttctacaa attgctctgc attcgcgaca gcattagtta atgaatccat agcattttta
     5581 tttctaatat aactataaaa gaaggacgcc ccaaagacat caccgcatcc aataatattt
     5641 ggattgataa ttttctttgc tgctacaaag tatgaagcaa tttcattttg atttttataa
     5701 tagatttttg ccccaagatc gccttttgta acactaatta ttttaactcc tattgcaaga
     5761 atatcttcaa caatatccat ctctgatttt ttattggata aagtaaacaa ttcgtgttga
     5821 ttaacttgaa ttatgtctaa acatttagcc caattatcga aatcaggtat cagcctaaac
     5881 tctcttttgt aatctgcact taatccgcgc gataaagtat gaatatcaat aaagattaat
     5941 cccgaataat tattcctaat ttgtctcaac tgatcaagcg taatatcaaa tccggtgatc
     6001 atattaatca aaattccatc aaagctaatt aaatccgaaa aattaagtga aagattattc
     6061 gtaatgtttt cataagcttc gtgcctttct ctatcttttt gcagattgag atgaaccctt
     6121 ggaatctttt caactttctg aatgaactta tcattcactt tttcaaattc cggcttgaag
     6181 tatttataag tctcatcatc aaattgagaa caaaggaata tttcttcatg ggcagatttt
     6241 aatctgttaa gtgcagagat agaataaaat attccgcctg cacttatatt ctgttctcta
     6301 tcagatttaa taaaatccag aactgaatgt ccaattacaa gtaatttcat ttttataaac
     6361 tttgtctgca caaaattacc attgattttg atactatcta aatcacatct gttaaataag
     6421 tgttaaacta agttcataat tcaatcaaaa tgctatatat ttgaaaacaa atcttacaaa
     6481 ataattatta tgaaaatcgg aataacctgt tatcctactt acggcggaag cggtgtaatt
     6541 gcaacagaat taggtaaaga attagcttta cgcggtcatg aagttcactt tattagttat
     6601 gcacttcctt tcagacttac aaaatatgta gagaatatat tttttcacga agttgaaaca
     6661 agcacttatc cattatttga gtttccactt tatagcttat cacttgcaag caagatgatt
     6721 gagattgcag agtttgaaaa tttagacttg ttacacgtac attatgctat tccgcatgca
     6781 acatcagctt atctggctaa gcagatcgtt aagaataaaa aacttaaaat aaccacaacg
     6841 cttcatggaa ctgatataac cctagtagga ttagaacctt cttttctgcc acttgtaaaa
     6901 ttcagtatag agcagagtga tggaattaca gctgtttcaa gatttttaaa ggaaaaaaca
     6961 cttacaaatt attcgattga aaaagaaatt actgttatac caaattttgt agataccgat
     7021 gtatttaaga aagtttctat agatgattgt gctttcagaa aacacattgc cccaaatggc
     7081 gaaaaggttt tagtacatac atcaaacttt cgtcccgtaa aaagagttac agatgcaata
     7141 agagttttta atatcgtgca aaaagaagtc ccctcaaaac ttttacttgt tggggatgga
     7201 cctgatagat cagaatgtga aagacttaca agagaattaa atctcaccga ttgtgttaaa
     7261 ttccttggaa agcaggaggg actcgtagaa atcttaagtt cttctgatgt gtttttaatt
     7321 ccctcgcagt cagaaagttt tgggttggct gctctggaag ctatggcttg cggactaccc
     7381 gtggtaagtt caagtgtagg cggattaccc gaattagtaa aacataatga gtgcggattt
     7441 attgcagaga tcggtgatat tgagagaatg gcaaaataca cactggatct tttaacaaat
     7501 gataaaaagt atgaaatttt ttctcagaat gcccgtaaca gaactttaaa caattttgat
     7561 aaatcaattg ttgttcctct ttatgaacaa cactataaaa atattttaga acagtaagat
     7621 gataatttat tgcgcaggac caatcagggg aaatactaca tatcaagata attattctga
     7681 gattgtaaga atcgttgaat caatgggaca cactgccctg tctgaaaaaa gcagcaaatt
     7741 cagttcttca attatgctga gtgataaaca agtgtatacc cgtgatatta agtggatcga
     7801 tgcaagtaaa attgtaatag ctgaagtctc cggcccaagt ttaggagtag gctttgaggt
     7861 ttcatatgct ttattcgtta aaaaaatgcc ggtgcttgca gtttatcacg atcaagcagg
     7921 acaaatctct gctatgataa ccggctgcac tgatccgaaa ttgcaaataa aaaaatattc
     7981 aaacgttgat gacctcacaa gtattataaa gaaatttatt cttagcaacg gcggcaattg
     8041 aacgaaaaac tttttatcga aagctggtta aataaaattc aaactgccgg attaaagcaa
     8101 ttcccacttc attttatcga tgaaacccga ctcgacatta tctctattcc aacaaaaact
     8161 ttagtgattg gacaggaatt ttttggtgcc tatgaaatta ttacgacgga tggtgagtcc
     8221 gtttatcaag cttcaaatta tgatgaagca aaatttattg tttacagctc aaaagaaaga
     8281 aacggcaaat cgtatcttcc caaagacaga tcgatgatta aaacactcgt tgattcatac
     8341 aataattatc tggatgagct gcttggtcaa ataaaaaaag attataaaaa gaattttcag
     8401 gatggaaaaa atgttcacgg tgtttccaat gaaatattta aaaaactaaa tctcatcagg
     8461 tattaatttt ggcaacagaa gtctcgcaaa aaatctacga ttatttttct tcattgtatg
     8521 ggaaagagtt tgccgataag tacatacaat ttattgatca ggatagcgcc cagtatataa
     8581 gggttaacac cgcaaaaatt acaagagaag atttatctca attacttttt gaaaaatatc
     8641 aaattaaaac tttagctgat aaacattttg aaaaagtatt aaaaataatt gagggcaatg
     8701 ataggatagg aaaaacattt gaacacgtat tgggatttta ctacatacaa tccctttcct
     8761 caatgatgcc tccattgatt ctcaatccaa caagcgagga catcgtttta gatctatgtg
     8821 gtgctcccgg ttctaaatca acgcaaattg ctgagattat gaacaaccgc ggcgcacttt
     8881 taatcaatga agtggataac gaaagaatta aatcgcttgt ttttaacctt gaaagaatga
     8941 acgttatcaa tgcatctgtt attcattcaa agggtgaaat cctcagcaaa gtttatgatg
     9001 attattttac taaagttctt gttgatgcac cctgcagcgg acttgggata atccaaaaaa
     9061 aaggcgaagt aagcaattgg tggtcgctcg atcatgtaga cagacttcag catcttcaaa
     9121 tgagattact tattgctgca ataaaaatgg caaaagttgg agcagaaatt gtctactcaa
     9181 cttgcactct ttctgttgaa gagaatgaat tagttattga taaaatatta gaaaaatatc
     9241 cagttgaatt attagaaata gatttgccga ttccatcacg accggccttt actgaatatg
     9301 atggaaaaca attaaatccc gaattaaaaa aagctatccg tattcttccg tgggaaattg
     9361 attccgatgg attcttttta gttaagatga gaaaagttaa tgttacagac tcaccggaaa
     9421 aagacgatgt aagagaaact gagaatagaa ttgttaaaag taatcataaa gaaattcagc
     9481 agtatataaa ttatatttcc gagcactttg gtattgatag aaaagtgtta gatgattaca
     9541 aatatatttt caggggtaaa gatattttct ttgtaattaa agattgggat gaagaacaca
     9601 tcggtttatt taaccgaatt ggattgaaac tcgggacact tgataaaaag gatagaataa
     9661 catttaattc tcaggctgct caggttcttg aaaaatacat cacaaaattc atttatgaat
     9721 taaagaatga agatgaatta aaaaattatt taaccggatg gaaaattaaa gagattgata
     9781 ttccgcttgg gcagtacgtt gttaaatata ataattggat tttagggaca gccgtaatga
     9841 cagttgaagg attaaagagc agattcccgc gaacaaaacg tacacaaaaa ttcgaattct
     9901 agtttcttat ctcaataaaa tcatcttttt tgtttgaaca aaaccccgtc ccgagcctgt
     9961 cgaaggatcg ccaacctgta atttataaaa atacactccg cttgctcgaa tcgattcgtt
    10021 tgctaaatta aactcagcct cataagtccc ggctggtctg tattcattaa gcaatgttac
    10081 aacctcattt cccaaaacat cataaacttt taaagtctgc catccggcga taggtgattg
    10141 ccagctaatt ttagtacttg gattaaaagg gtttggataa ttctgataca actcaaaatc
    10201 ttttatctct gtaatttcat tctcaacatt ggttgcagta acgggtgaaa gttttaaaat
    10261 tccattatca ggaataactt tggcaagaac aacatttccg gaatcagtta ctattgaggt
    10321 taaagtatca acaattccat tttgctccgt tctaacatac tcccaattat ttggaacttt
    10381 tacataagca cttaaaggat aattaaatat tgcattctct aaattatcag aaacattaat
    10441 ttgaatcagt tggtttgaag atgatataat ttgatattgt gcatcatctc tttcctttat
    10501 gtagcgggta atattgccaa cagtttcaac ccaaacttct ttatccgatg atcttgccca
    10561 taaaaaatca caaagcgaag ttaaccattc atttgtaacc ggttcataaa taccttgatc
    10621 taataaatct tgaagttgat caaatggaac aacatcatga ataattatca ttccccattt
    10681 atgattatta atagaatttt gcgtccattc taaaaaagag taaagctcgt ccagatcatc
    10741 agccacagaa tttctcggct cactaaactc aacaacttta gcttttaacc caaaccagtc
    10801 ttcttcagat aatgaagaat cgttagggac ttgccctaga gttcttccat tctcataaaa
    10861 taaacgtgca gcagaatcca caacagaatt atgtattgta tatggatagt ttagtgatat
    10921 gcatttttct gcgggaattt tttgctgaat aaataattgg gattgacata attcatacaa
    10981 caaagtgccc tcatcattta ttgatcccca taaaagcgaa gttaaatcgt aatggttcat
    11041 tgtatgcgaa ctaatttcgt gtccttcggc tgccaatgat tgaaaaccgg gccaggtgcc
    11101 gtatctccaa attccaggaa gtgtttcagt taagtatgga ggaagaacat aaaacgtccc
    11161 tttaattcca tattgattta aaatcggccc aacattttca atgtgtgtaa gcaatccatc
    11221 atcaaaagtt aggctgaatg cgctttgcct gtcatttgca taattagtaa aacgcaatgt
    11281 tccataacct tgtgcatgcg aaatctgagc aattagaaat attgtaaaaa gtatttgtct
    11341 gatcatcagt aatatttatt taatgcttaa agatattctt ttttgagtaa ataacttgat
    11401 caaaaatgat gcctttaatc acatactaca actaatattt tcgtcataaa atcgttggtt
    11461 caacaatggt atgaatgatt ttgataatta ttatcttgcg ccgtcttttt ttgataaact
    11521 gattttgcaa aatgttaaaa cccaaattat ttactacact taaagattat acttcaaaac
    11581 aatttattgc tgatctatct gcaggaacaa ttgttggcat cgttgccctc cctctggcta
    11641 ttgcattcgg aattgcctcc ggtgttacac cggaaaaagg attgattac
//
LOCUS       DASWBY010000601         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_58968, whole genome shotgun sequence.
ACCESSION   DASWBY010000601 DASWBY010000000
VERSION     DASWBY010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58968"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /gene="gdhA"
                     /locus_tag="VF870_10970"
     CDS             complement(<1..1259)
                     /gene="gdhA"
                     /locus_tag="VF870_10970"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX9252754.1"
                     /translation="MSDYTNKLIADVKAKNPAEPEFHQAVEEVMGSLELVLQRHPEYR
                     SMKILERMVEPERTVTFRVPWMDDQGEIHINRGYRIEMNSAIGPYKGGLRFHPSVTMG
                     ILKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELSRHIGPNT
                     DVPAGDIGVGGKEIGFLFGQYKRLRNEFTGVLTGKGINWGGSLIRPEATGYGVVYFTQ
                     EMLKTKNTTFDGKIVAVSGFGNVAWGAVQKVTELGGKVVTLSGPDGYVYDPDGIKGEK
                     IDYMLKLRASNKDAVEDYAKEYKVKFVPGKRPWEVKCDVALPCATQNELDENDAKELI
                     KNGCICVCEGANMPTTIEGFKVFTDAKILYAPGKASNAGGVATSGLEMSQNSMRLPWN
                     REEVDLRLHQIMVRIHETCITTAEKYGT"
     gene            1585..>2001
                     /locus_tag="VF870_10975"
     CDS             1585..>2001
                     /locus_tag="VF870_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX9252755.1"
                     /translation="MSLEIIKEKVAQAVDILNEKNIDMWVTFVQETKVLKDPIMDMTV
                     GDHSTWKSAFIINKDGDTTAIVGDMELENFVKSGVYKNIIGYLKSFKEPFIEYVKKKN
                     PQTIAINFSVNSVLSDGLTHGMYLLLMEYLKGTGYEN"
BASE COUNT          614 a          368 c          321 g          698 t
ORIGIN      
        1 gttccatatt tttcagcggt ggtaatacag gtttcatgaa ttctaaccat aatctggtgt
       61 aatctaagat caacctcttc tctgttccat ggtaaacgca ttgaattctg tgacatttct
      121 aatccggagg ttgcaacacc gccggcattt gaagctttac cgggagcata aagaatttta
      181 gcatccgtaa atactttaaa tccttcgata gttgtcggca tatttgcacc ttcacaaaca
      241 caaatacatc cgttttttat taattccttt gcatcatttt catcaagttc gttttgtgtg
      301 gcgcatggta atgcaacatc gcatttaact tcccaaggtc ttttaccagg tacaaatttt
      361 actttatatt cttttgcata atcttcaact gcatctttat tgctcgcacg taacttgagc
      421 atataatcaa tcttttcacc ttttattcca tctggatcat aaacatatcc atctggtccg
      481 gaaagcgtta caacttttcc gccaagctct gtaacctttt gaaccgcgcc ccatgcaaca
      541 ttaccaaatc cggaaacagc aactatctta ccgtcaaatg tggtattctt cgtttttaac
      601 atttcttgtg taaaataaac aacgccatat cctgttgctt caggtctgat aagtgatcca
      661 ccccaattta ttcctttacc cgttaacaca cctgtaaatt catttcttaa tcttttgtat
      721 tgcccgaaca agaaaccgat ttcttttccg ccaacaccaa tatcaccagc aggcacatct
      781 gtatttggac caatgtgtct tgaaagttct gtcataaagc tctggcagaa tctcataact
      841 tcgttatcag attttccttt aggatcaaaa tcagatccgc ccttgccacc gcccattggt
      901 aatgtagtta aagagttttt aaatacttgt tcgaatgcta aaaattttaa aatgcccatt
      961 gtaactgaag ggtgaaatct taacccgcct ttgtaaggac cgattgctga attcatttca
     1021 attctgtaac cacggttgat atgaatttcg ccttgatcat ccatccatgg aacacggaat
     1081 gtaacagttc tttccggttc taccattctt tctaaaattt tcattgaacg atactcagga
     1141 tgtcgctgta aaactaattc aagtgaaccc atcacttctt caaccgcttg atggaattcc
     1201 ggttctgcag gatttttagc tttaacatca gctattaatt tatttgtgta gtcagacata
     1261 aaaagacctc tatttttgtt tgttgttcat caatgatgaa ttaagtttgt gataaataat
     1321 tttttattaa ttgtataaat ggacagaaaa ttttcggagt gtaaaacact ttttccccta
     1381 ggaaatatta ctgttgcgtt tagcatttag tgttaaaatc aatattttta atacatcaaa
     1441 attagaaaga aaataagtaa ttccctaaat aattaaggca gattagagga gtattatttg
     1501 atttttcgaa ttattttgat ggtggaattt ttcaatcaat aaatgtgaat taactgttta
     1561 tctaaattta ttagtgaggt tattatgtcc ttagaaataa ttaaggaaaa agttgctcaa
     1621 gctgttgata ttttaaatga aaaaaatatt gatatgtggg ttacatttgt acaggaaacc
     1681 aaggttttga aggatccaat tatggatatg actgttggag atcactcaac ctggaaatca
     1741 gcttttatca tcaataaaga tggtgataca acggcaattg ttggtgatat ggagcttgag
     1801 aattttgtta aatcaggcgt ttataaaaac attattggtt acttaaaatc ttttaaagaa
     1861 ccatttatag agtatgttaa gaaaaaaaat ccccagacaa ttgcaattaa cttttctgtt
     1921 aactctgttc tttctgatgg cttaacacac ggaatgtatc tgctgttgat ggaatatctt
     1981 aaaggaacag gatacgaaaa t
//
LOCUS       DASWBY010000602         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_286360, whole genome shotgun sequence.
ACCESSION   DASWBY010000602 DASWBY010000000
VERSION     DASWBY010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286360"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VF870_10980"
     CDS             <1..590
                     /locus_tag="VF870_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9252756.1"
                     /translation="KKGDTVVDLGSGAGNDVFVARAIVGDEGKVVGIDFTNEMIIKAN
                     INLDKLGYKNVEFKLGEIEALPLEENTADVVVSNCVLNLVPDKQKAFNEIYRVLKPGA
                     HFCVSDIVIKGELHPELKKSAEMYAGCVAGAVQQEEYLGIIKSTGFVNAEIKKTKTIV
                     LPDEVLTKYLNKNQLAEFKEKQVGIFSITVVGYKN"
     gene            757..1179
                     /locus_tag="VF870_10985"
     CDS             757..1179
                     /locus_tag="VF870_10985"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX9252757.1"
                     /translation="MRILVLCTGNSCRSQMAEGFLKSFDKNFEVYSAGTNPAEKVNPF
                     AVKVMQEIGIDISNGNAENVNKYLSQSFDYVITVCDNAKETCPVFIGNVKHELHIGFD
                     DPADAVGTEEEIMPVYRSVRDEIKKDFYEFYLKELKDK"
     gene            1176..2234
                     /gene="arsB"
                     /locus_tag="VF870_10990"
     CDS             1176..2234
                     /gene="arsB"
                     /locus_tag="VF870_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX9252758.1"
                     /translation="MRSSKKRLKFLDRYLTLWIFLAMFIGVFSGYLFPSVDSFWNSFQ
                     SGTTNIPIAVGLILMMYPPLAKVKYEELPDVFKNVKVLTLSLVQNWIIGPMLMFVLAI
                     IFLQDYPEYMAGLILIGLARCIAMVIVWNELAKGDTEYAAGLVAFNSIFQVLFFSIYA
                     YVFLTVIPSWLGLKTFAVDITIGQIAKSVFIYLGIPFLAGMITRFVLLKIKSKQWYEQ
                     KFIPKISPITLIALLFTIVVMFSLKGEYIVKIPLDVIRIAIPLLFYFVIMFFISFYLG
                     KKIGADYSKTATLSFTAASNNFELAIAVAIAVFGINSGEAFAAVIGPLVEVPVLIALV
                     NVALWMREKYFKEPITTI"
BASE COUNT          764 a          311 c          448 g          818 t
ORIGIN      
        1 ttaaaaaagg tgacacagtt gttgatttag gttcaggtgc tggtaatgat gtttttgtag
       61 ctcgcgcaat tgttggcgat gaaggaaaag ttgttggaat tgattttact aatgaaatga
      121 taatcaaagc aaacattaat cttgataaac ttggctataa aaatgttgaa tttaaacttg
      181 gagagattga ggcattacct ttggaagaaa acactgcaga tgttgtagtt agtaattgtg
      241 ttttaaatct tgttcctgat aaacaaaaag catttaatga aatttatcgt gtgctaaaac
      301 caggtgcaca tttttgtgtt tcagatattg ttataaaagg cgaactgcat cctgagttaa
      361 aaaaatctgc agaaatgtat gctggttgtg tagctggtgc agttcaacaa gaagaatatc
      421 ttgggattat caaatcaacc ggttttgtga atgctgagat caaaaaaaca aaaacaattg
      481 tattaccaga tgaagtttta actaagtatt taaataaaaa tcaattggca gagtttaaag
      541 aaaaacaggt tggaattttt agtattacgg ttgttgggta taagaattaa aaagataaat
      601 cattagaaaa acaaatttga ttgaaaatta ttttttgtca tttcgatccc gatcttaatc
      661 gggagagaaa tctgtattaa ttataagact tttccctttg gtcgaagtga cagacacaat
      721 attaacaaaa ctctaagggt aagaaacaat aatcatatga gaattttagt tttgtgtacc
      781 ggcaattcct gccgtagcca gatggcagaa ggatttttaa aatcatttga taaaaatttt
      841 gaagtctatt cagcaggaac aaatccggca gaaaaagtaa atccattcgc ggtaaaagta
      901 atgcaggaaa ttgggataga tatcagcaat ggaaatgctg aaaatgtcaa taaatatctt
      961 tcacaatctt ttgattatgt aattacagtt tgtgataatg caaaagaaac ctgtccggtt
     1021 tttattggaa atgtaaaaca tgagttacac attggatttg atgatcccgc tgatgctgtt
     1081 ggtaccgaag aagaaattat gcctgtttat cgaagtgtgc gtgatgaaat aaaaaaagat
     1141 ttttatgaat tttatctaaa agaattgaaa gacaaatgag atcatctaaa aaaagattga
     1201 aattcttaga cagatattta acactctgga tttttcttgc aatgtttatt ggggtgtttt
     1261 ctggttactt gtttccatca gttgatagtt tctggaattc atttcaatcc ggcacaacaa
     1321 atattcctat agcagttgga ttgatattaa tgatgtatcc gcccctggca aaagttaagt
     1381 acgaagaatt acctgatgtt tttaaaaatg taaaagtgct tacgctttct ttggtgcaaa
     1441 actggatcat cggtccaatg ctaatgttcg ttcttgcaat aattttttta caagattatc
     1501 cggaatacat ggcgggattg attctgatag gacttgcaag atgtattgca atggttattg
     1561 tttggaatga acttgcaaaa ggggatactg aatatgctgc tggacttgtc gcatttaatt
     1621 caatctttca ggttttattc ttttcgattt atgcatatgt gtttttaact gtcattcctt
     1681 cgtggcttgg actaaaaact tttgctgttg atatcacaat cgggcaaata gctaaaagtg
     1741 tttttatcta tcttggaatt ccatttttag ccgggatgat aactcgattt gtcttactta
     1801 aaataaaaag taaacaatgg tatgaacaga agttcattcc aaagatatca ccaattactt
     1861 tgattgctct gttatttaca attgtagtta tgttttcatt aaagggagaa tatattgtta
     1921 agattccgct tgatgtgatt agaattgcca ttcctttact attttatttt gtgataatgt
     1981 ttttcatttc attttatctg ggcaaaaaaa tcggagctga ttacagtaaa actgcaacgc
     2041 tttcgtttac tgcagctagt aataattttg aacttgctat agccgttgcg attgctgtat
     2101 ttggaattaa cagtggggaa gcatttgccg ctgttattgg tcctttagta gaagtgccgg
     2161 ttctaattgc tttagttaat gttgcattgt ggatgcggga aaaatatttt aaggaaccaa
     2221 tcactacgat ataattatca tctacaagtt aaatttagtt tgactgattt ttcttatatt
     2281 tgcaccagca tgtttaagaa taaaaattat tagctcgcat ttcaactcta tttaggcttt
     2341 t
//
LOCUS       DASWBY010000603         6594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_478828, whole genome shotgun sequence.
ACCESSION   DASWBY010000603 DASWBY010000000
VERSION     DASWBY010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478828"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VF870_10995"
     CDS             <1..231
                     /locus_tag="VF870_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HEX9252759.1"
                     /translation="GSLGVMADIIAVHGPDSFIGILVSTFFGSSETTFYVLAVYFGAV
                     NIKNTRHALAAGLAADIAGALGALFIVKLLFG"
     gene            365..952
                     /locus_tag="VF870_11000"
     CDS             365..952
                     /locus_tag="VF870_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252760.1"
                     /translation="MFENNDRLSCKINETLISDYGVSIDNANMGTYWEAVVYFSKDDF
                     TLNAFIQDGDGVKETEVLKIPLKKVNDIKIFEVSYGTLKTIFKKAVIAAAISSIIISI
                     LMLLHPEPGYTDIGFILPVSLFLGIGLTLVFLALLFFFHQPKKNICINIITNDKRLIT
                     FFTDIEQKEWVMKILLKYFKEVENKGFPYQDLDLN"
     gene            1228..2022
                     /locus_tag="VF870_11005"
     CDS             1228..2022
                     /locus_tag="VF870_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252761.1"
                     /translation="MDINLFLEKIPLLYLYFITAAAVFLSIMIGYRVGIYVQNRRKIT
                     KDAKLGSIIGAMLGLLAFILAFTFGAATSRFDDKKQLLLDEVNAIGTTFLRTDFLAQP
                     ERADARKLLKKYVDLRVNILKNPKQLPQVIIESEKIQSMLWSQVSSVSNKQSDPVLLS
                     LYIQSLNDVIDLHTKRVTVGLQYRIPGNIWMTLYFVTILTMLAVGYEFGLNGAGSNLI
                     SLILALAFSAFILLIADLDRGVAGTLRVSQKPLIELQQKLNNSLDK"
     gene            2043..2465
                     /locus_tag="VF870_11010"
     CDS             2043..2465
                     /locus_tag="VF870_11010"
                     /inference="COORDINATES: protein motif:HMM:NF040341.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HEX9252762.1"
                     /translation="MIKISLFFFILLFFFSCNKEQVSESNAITSYELWRSKNFHNYSI
                     DQSRSCFCPDAGELVRITVRSDSIFSVIKISDNSVITSSHYFTVDSLFGIINNSGNDS
                     LAVRYNAFYGYPEFLDINPQLHPVDGGVLYETSNLQKQ"
     gene            2543..3493
                     /locus_tag="VF870_11015"
     CDS             2543..3493
                     /locus_tag="VF870_11015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX9252763.1"
                     /translation="MKIFITRIIPSIGIELLKKKGFEVFVYKQDKSIPRKDFIEAIKN
                     CDAVISLLTDKIDKEVIDKMKSCKVIANYAVGYNNIDIEYARSNGIVVTNTPDVLTDS
                     TSDLTMALVLNCARRIPESEKLIRSGKFVGWKPQLLLGMELRDKYFGILGAGRIGTEA
                     AKRAHAFGCKIIYYSNNKNTFLESKLTAKKLSLNSILKKSDILSIHLPLTPKTYQLLN
                     KNNLHLLKKSSILINTARGEIVDEKVLIAMLKSKKIFAAGLDVYENEPNIKKEFFDLE
                     NVILLPHIGSATNESRNNMSLLAAKNVIAVLSGKKAITLV"
     gene            3562..4542
                     /locus_tag="VF870_11020"
     CDS             3562..4542
                     /locus_tag="VF870_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX9252764.1"
                     /translation="MRFGIPKETLYEEKRVALAPAGVDALVRSGHTVYIQNGAGEGSH
                     FSDEEYRRTGAQIVYSAEEAFARAEVVVKVAPLSENEAELLQENQTLFSFLHLAIGKR
                     KIIDHLLKKKITAIGYELIEEDDRLPVLHSMSEIAGQLSIQVAERYLESFSEGGRGIL
                     LGGITGVAPAAVVIIGAGVVGVTAARSAIGRGAQVIVIDKDLNRLRDIDRNFSKSVTT
                     VVANPYTISRGVKFADVLIGAVLIKGEKAPHLVTESMVKTMKKGSVIVDVSIDQGGCI
                     ETSHITTQSDPVYRLHDVIHYCVPNMPAIVSRTASYGLNNASLVYIQDIA"
     gene            4732..6087
                     /locus_tag="VF870_11025"
     CDS             4732..6087
                     /locus_tag="VF870_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9252765.1"
                     /translation="MTSTEEIPVKNPIPTNLLRIYNQKLVSSDEAVKCIKSGDNVIVQ
                     PGCAVPSELVRAMVRRKDELENITLYHILIVGDLPYLQPGMEKHFKHKAFFIGANARK
                     SVNEGRSEFIPIFLSEVPLLFKLGRIKTDVALLNVSPPDEHGFCSYGVDVGTIKTPAE
                     KSKIIIAQVNKYQPRTLGDCFIHINKINYIVEHDEELKELPQVDPDATPEVLAVFDKI
                     GRNVADLIEDGSTLQMGIGAIPDSVMKYLFDKKDLGIHTEMFSDGMIDLVDAGVINGE
                     KKTLHPGKIIAGFVLGTRRVYDFIDNNPVIEFHPQEYVNDPFIIAKNNKMVAINSALE
                     VDLTGQVCSDSIGTRFYSGIGGQVDFIRGASKSEGGKPIIALPSTTKNDTISRIVPTL
                     KPGAGVVTSRGDVDYVVTEYGSADLWGKTVQERAKALISIAHPNFRDELTKYAQETFH
                     I"
     gene            6094..>6594
                     /locus_tag="VF870_11030"
     CDS             6094..>6594
                     /locus_tag="VF870_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9252766.1"
                     /translation="MVAVIGDVHGCYYTLKKLLDKIRTKYPDIKIYCVGDLVDRGNYS
                     FEVIQFVIAEKINFTSGNHDYMFYSNMRDPFSLMAKSWRYNGAETTLASYKDKLNQME
                     EHLDLINNSPLFFNIDDCFISHAGISKSLKELLPENFLSNDAALKEILSNDLFNQNSI
                     IWARGPL"
BASE COUNT         2236 a          980 c         1223 g         2155 t
ORIGIN      
        1 ggttcacttg gtgtgatggc tgatattata gctgtccacg gaccagattc atttattgga
       61 attttagttt caactttctt cggcagttct gaaactactt tttatgtgct ggcagtttat
      121 tttggtgcag taaatataaa aaacactcgt catgctcttg ccgcaggatt ggctgccgat
      181 atagccggtg cgctaggtgc tttgtttatt gttaagcttt tgtttgggtg agataaataa
      241 ataactatca accagaagta atattatggt tcagtgtaag taaaagttag gtatttatta
      301 ctcagtatct aaattatttt acatcttttt cttttctaca aatagattaa gctgtttgaa
      361 caaaatgttt gaaaataatg accgtttgtc ctgcaaaatt aatgagacac tgatatcaga
      421 ctatggtgtt tcaatagata atgcaaatat gggaacttac tgggaggctg ttgtatattt
      481 tagtaaagat gattttactc tgaatgcctt cattcaagat ggtgatggag ttaaagagac
      541 tgaagtcctg aaaattcctt tgaaaaaagt aaatgatata aaaatatttg aggtgtctta
      601 cggaactcta aaaacaattt ttaaaaaagc cgtaatagct gctgctatct caagtattat
      661 tatatcaata ttaatgcttc ttcaccctga acccggttat actgatatcg gttttatatt
      721 acctgtttca ttgtttttgg gcattggcct tacacttgtt tttttagcac ttctattttt
      781 cttccatcag cctaaaaaaa atatttgtat aaatattata accaatgata agagactaat
      841 tacttttttt acagatattg aacaaaaaga atgggttatg aaaattcttt taaaatattt
      901 taaagaagta gaaaataagg ggtttcctta tcaggacctt gatctgaatt gaaaaatata
      961 tctaattaat tacctgacaa tcaaatcaac aaaacttcct tccaacaaga acagattgct
     1021 tcgataatct ttttcccggt aatgttgtga cgaaaataaa ttatatttgc aacgatattt
     1081 ttatttcgta ttcaaatgaa aaattaatat tgaaagatgt aaggcactaa attcaaattg
     1141 ctgttttacc gcttcagcca gatatttaat gtatattaag attcatttaa actagttatt
     1201 tttgaaatta taaagtgatt aaaaattatg gacataaatt tatttctcga aaaaattcct
     1261 ctgttgtatt tgtatttcat aactgcagca gcagtctttc tctcaataat gatcggatac
     1321 cgcgtaggaa tttatgtcca gaatcgcaga aagataacaa aagatgctaa actaggctct
     1381 attatcgggg ctatgcttgg tctgctcgca tttattcttg cttttacatt tggagcagcg
     1441 acatcccgtt ttgatgataa aaagcaatta ctattagatg aagtaaatgc aatcggtaca
     1501 acttttttac gtacggattt tcttgctcaa ccagagaggg ctgatgcaag aaagcttcta
     1561 aaaaaatacg tcgatttaag agtaaatatt ctaaaaaacc caaaacaatt accgcaggtt
     1621 atcattgaat ctgaaaaaat tcaaagtatg ctttggtcac aagtttcatc tgtatcaaat
     1681 aaacaatcag atcctgtatt actaagtctt tatatccaat ctttaaatga tgttatagat
     1741 ttgcatacaa aacgagttac agtcggactt caataccgca taccgggaaa catctggatg
     1801 acactttatt ttgttacaat tcttacaatg ctggcagttg gatatgaatt cggtcttaac
     1861 ggagcgggta gtaatcttat aagtctaatt ttggcgcttg cgttttcagc atttatacta
     1921 ctaattgccg atcttgatcg tggtgttgcc ggcacgcttc gggtgagtca gaaaccatta
     1981 attgagcttc agcaaaaatt gaataactcg ctggataaat aattttttag gagaataaaa
     2041 ttgtgataaa aatatcttta ttttttttca ttttattatt ctttttctct tgtaataaag
     2101 aacaagtttc agaatcaaat gcaataactt cgtatgaatt atggcgttca aaaaatttcc
     2161 ataattattc cattgatcaa tcccgttctt gtttctgtcc ggatgccggt gaattggtgc
     2221 gtataaccgt gcgctctgat agtatattta gcgttataaa aatttcagac aatagcgtga
     2281 ttacatcttc acattatttt actgttgatt cactatttgg aattatcaat aactccggga
     2341 atgattcact ggcagtaaga tataatgcat tttacggata ccctgaattt ctggatataa
     2401 atccgcaatt gcatccggta gatggtggag ttttatatga aacttctaac ttgcagaagc
     2461 aatgaaatta aacatgccgg tttgattttt aatgatagat tgataaataa aaagttaatt
     2521 aaaatcgaaa caaagatatt ttatgaaaat ttttattaca cgaataattc cttcgattgg
     2581 aattgaatta ttaaagaaaa aaggatttga ggtctttgtt tacaaacaag ataaatcgat
     2641 tccgcgaaaa gattttattg aggcaattaa aaactgtgat gctgtaatat ctttacttac
     2701 agataagatt gataaagaag taatcgataa aatgaagagt tgcaaagtta tcgcaaacta
     2761 tgccgttggt tataacaata ttgatattga atacgccaga agtaatggga ttgtcgttac
     2821 aaatactccc gatgttctta ctgattcaac ttcagattta acaatggcac ttgtgttaaa
     2881 ctgcgcaaga agaatccctg aatcagaaaa acttattaga tccggaaaat ttgttggatg
     2941 gaaaccgcag ttacttttgg gaatggaatt acgagataag tatttcggaa ttcttggtgc
     3001 aggaagaatc gggactgaag ctgcaaaaag agcgcatgct tttggctgta agataattta
     3061 ctattcaaat aataaaaaca cttttctcga aagtaaatta actgcgaaaa aattatcgct
     3121 taactcaata ctaaaaaaat ctgatattct ttcaattcat cttccgctta ccccaaaaac
     3181 atatcagtta ctaaataaaa ataatctgca tctgttaaaa aaatcttcaa ttcttattaa
     3241 tactgcacgt ggagaaattg ttgatgaaaa agtattgatt gcaatgttaa aaagtaaaaa
     3301 aatatttgct gcaggtctgg atgtttatga gaatgaacca aatattaaaa aagaattttt
     3361 tgatttagaa aatgtaattc ttttacctca tatcggcagc gctacaaatg aatcaagaaa
     3421 taatatgtca ttgcttgcag caaaaaatgt tattgcagtt ttatcaggaa aaaaagcaat
     3481 tacactggtt taatttccat caaattgatt gctaatctta tcttccaatg gtaattttaa
     3541 aacaaaaaaa gagaaaatta aatgagattt ggaataccaa aagaaactct ttatgaagaa
     3601 aaaagagttg cattagctcc tgccggagta gatgctttag tacgatccgg tcatacagtt
     3661 tatattcaaa atggtgcagg tgaggggagt cattttagcg atgaggaata tcgcagaacc
     3721 ggcgctcaaa ttgtttactc cgctgaagag gcttttgcca gagcagaagt ggttgtaaaa
     3781 gttgcaccgc tttcagaaaa cgaagcagaa ttacttcagg aaaatcaaac tctcttttca
     3841 tttcttcatc ttgccatagg taagagaaaa ataattgatc atcttcttaa gaaaaaaatt
     3901 accgcaattg gttatgaatt aatcgaagaa gatgacagac ttcctgtgct gcattcaatg
     3961 agtgaaattg caggacagct ttctatacag gttgctgaaa gatatcttga aagttttagc
     4021 gaaggcggta gaggtattct gcttggcgga attacaggtg ttgcgccggc tgcggttgtt
     4081 attattggtg caggtgttgt cggtgttact gccgcacgtt cagcaattgg aagaggtgca
     4141 caagtaatcg tgatagataa agatttaaat agattaagag atatagatcg taattttagt
     4201 aaaagtgtta ctacagttgt agctaatcct tatacaattt ctcgtggagt taaatttgct
     4261 gatgttctta ttggagcggt tttgattaaa ggagaaaaag caccgcatct tgtaactgaa
     4321 tcaatggtaa aaaccatgaa gaaaggttct gtgattgttg atgtttctat tgatcaggga
     4381 ggatgtatag aaacaagtca tatcacaact caatcagatc ctgtatatag attgcatgat
     4441 gtaatccatt actgcgtacc taatatgcct gcaattgttt ccagaactgc aagctacgga
     4501 ttaaataatg cttcgcttgt ctacattcag gatattgctt aaaatggttt atcgaatgct
     4561 ttgcttggtg atatagggtt atcgaaagga gtttgcacct ataatggata ctgctgtaat
     4621 gaatccattg ctcatacttt taatgtagaa tatcgaagac tacatatttt ttcaagtaac
     4681 tagtagaaaa taaaacgaaa acgaattgaa tttaagaaat gagaatttga aatgacatca
     4741 acagaagaaa taccagtaaa aaatcctatc ccgactaacc ttttaaggat ttataatcaa
     4801 aaattagtaa gctctgatga agcggttaaa tgtattaaat caggtgataa cgtaattgtg
     4861 cagcctggat gtgcagtccc ttcagaatta gttagagcaa tggttagaag aaaagatgaa
     4921 cttgagaata ttacacttta tcatattttg atagtcggtg atttacctta cttacagccc
     4981 ggaatggaaa aacattttaa gcataaagca tttttcatcg gggctaatgc tagaaaatca
     5041 gtaaatgagg gaagatcaga atttattccc atctttttat ctgaggtacc actgttattt
     5101 aagttaggca gaattaaaac tgatgtggct ttgttaaatg tatctccgcc tgatgaacat
     5161 ggtttttgca gttacggagt tgatgtgggt acaattaaaa cgcctgcaga aaaatcaaaa
     5221 attattattg cacaggtaaa caaatatcaa ccaagaactc taggtgactg ctttatacac
     5281 ataaacaaga ttaattacat cgttgaacat gatgaagaat taaaagaact tcctcaggtt
     5341 gatccagatg caactccgga ggttttagct gtatttgata aaataggaag aaatgttgct
     5401 gatctcatcg aagatggatc gacattacaa atgggcattg gcgctattcc tgattcagtt
     5461 atgaaatatt tatttgataa aaaagattta gggatccata cagaaatgtt ttctgatggg
     5521 atgattgatc ttgtagatgc aggagtaatt aatggagaaa agaaaacact tcatcctgga
     5581 aaaattattg caggatttgt gctcggaaca agacgtgttt atgattttat tgacaataat
     5641 ccggttatag aatttcatcc gcaggaatat gtaaacgatc cattcattat tgcaaagaat
     5701 aataaaatgg ttgcgattaa ctcagctctg gaagttgatc ttacgggaca ggtttgttct
     5761 gattcaatcg gaacaagatt ttattccggt attggcggac aagtggattt tataagaggt
     5821 gcttcaaaat ctgaaggagg caaaccgatt atagcattgc cttccacaac aaaaaacgat
     5881 actatttctc ggattgttcc tacacttaaa cccggtgcag gtgttgtaac atccaggggt
     5941 gatgttgatt atgtggttac agaatacggt tcggcagatc tttggggaaa aacggttcaa
     6001 gaaagagcta aagctttaat ttcaatcgca catccgaatt ttagagacga attaacaaag
     6061 tatgcacaag aaacatttca tatttaggta aaaatggttg cagtaatcgg agatgtacac
     6121 ggttgttatt atacactaaa gaagttatta gataaaataa gaactaaata tcctgatatt
     6181 aaaatttatt gtgttggtga tcttgttgat cgtggaaatt acagttttga agtaattcag
     6241 tttgtaattg ctgaaaagat taatttcact tctggtaacc acgattatat gttctattca
     6301 aatatgcgcg atccattcag tttgatggct aaatcctggc gctacaatgg agccgaaact
     6361 acattagcct cttacaaaga taagcttaac cagatggaag aacatctgga tctgattaac
     6421 aacagtccgc tattttttaa tattgatgat tgtttcattt cacatgcagg aatatctaaa
     6481 tctttaaaag aattattgcc ggaaaatttt ctttcaaacg atgcagcgtt aaaagaaatt
     6541 ttgagtaatg atttgttcaa ccagaatagt attatctggg ctaggggtcc gcta
//
LOCUS       DASWBY010000604         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_174942, whole genome shotgun sequence.
ACCESSION   DASWBY010000604 DASWBY010000000
VERSION     DASWBY010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174942"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..2192
                     /gene="tkt"
                     /locus_tag="VF870_11035"
     CDS             174..2192
                     /gene="tkt"
                     /locus_tag="VF870_11035"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9252767.1"
                     /translation="MSDSRIDIEQLSINTIRTLAIDAVQKANSGHPGMPLGMAPIAYA
                     LYYKTLKHNPANPNWLNRDRFVLSAGHGSMLLYSILHLCGYKISIEDLKNFRQWSSIT
                     PGHPEINHTPGVETTTGPLGQGFATAVGMAIARDYLAALFNKDDIKIIDHAIVGICSD
                     GDLMEGVSHEAASLAGHLKLSKLLFFYDDNSITIDGKTDITYSDDVARRFEAYGWQVL
                     TILDANNSDQVDRAIKLCRTTCDKPTLIITKTHIGYGSPNKQDKSSAHGAPLGEEEVK
                     LTKRNLGMPEDKTFYVPDEVYSHFKGIAECGQEAQDLWDKNLEEYKILYPKDYALFES
                     VMNYEFDKEWLNHLPKFDDLSEKIATRVASGKVLNAAAIDIPTLIGGSADLNESNMTH
                     IKSSTSFSAEDRKGKNIHFGIREHAMGSILNGMAIYGGIIPFGATFLIFSDYMRPAIR
                     LAALMKQKVIYVFTHDSIGLGEDGPTHQPVEQIASLRAIPNLTVIRPADANETVEAWK
                     YAINHKGGPVALILTRQKLSIIDQNKYGSAKGLQNGAYILKDSNKNPDLLLIATGSEV
                     SLSLKAADQLEAEGKVVRVISFPSWEIFEQQSDEYKKKIFPAEIKARVSVEMGVGMGW
                     QKYVGDSGEAISIERFGASAPDSILYEKYGFTVENVVATSKRVLSKTK"
BASE COUNT          797 a          372 c          478 g          695 t
ORIGIN      
        1 ataaattctc cagaagagag ttttggaaat aactttataa tctttacata taaaaatgga
       61 ttgattaaga gattattgca aattaaaatg taatgacatt tacatataag acttcattaa
      121 ataaaaattt ttttatctta gcccatccaa tttttaatta aaccggaaac actatgtctg
      181 attctagaat tgatatcgaa caattatcca taaacacaat tagaactctt gcaattgatg
      241 ctgtacaaaa agctaactct ggtcatcccg gcatgccgct tggaatggcc ccgattgctt
      301 atgcacttta ctacaaaaca ttgaagcaca atcctgctaa tcctaattgg ttaaacagag
      361 atagatttgt tctttctgca ggacacggaa gcatgcttct ttactctatt cttcatttat
      421 gtggatataa aatttctata gaggatctta aaaattttag gcagtggagc agtattactc
      481 ccggacatcc cgaaataaat catacacccg gtgttgaaac aacaacggga ccattagggc
      541 agggttttgc aactgcagtg ggaatggcaa ttgcacgcga ttaccttgcc gcacttttta
      601 acaaagacga tataaaaatt attgatcatg caattgtggg aatatgcagt gacggcgatt
      661 taatggaagg tgtttcacac gaagctgctt cgcttgcagg tcatctaaaa cttagtaaac
      721 ttcttttctt ttatgatgat aatagtataa cgattgatgg taaaactgat attacttatt
      781 ctgatgacgt agccagaaga tttgaagcat atggatggca ggtgcttaca atattagatg
      841 ctaacaattc tgatcaggtt gatcgggcaa ttaaattatg cagaacgact tgtgataaac
      901 ctaccttaat aataaccaaa acccatattg ggtatggaag cccgaataaa caggataaat
      961 catcagcaca tggtgcgccg cttggtgaag aagaggttaa actgacaaaa cgaaatctcg
     1021 gaatgccgga agacaaaaca ttttacgttc ccgatgaagt ttattcacac tttaaaggaa
     1081 ttgccgagtg cggacaggaa gctcaagact tatgggacaa aaatcttgag gaatataaaa
     1141 ttttatatcc taaagattat gcgttgtttg aatcagtaat gaactacgag tttgataaag
     1201 agtggttaaa tcacttacca aagtttgatg atctttctga aaagattgct acacgtgttg
     1261 cctccggtaa agtattaaac gctgcggcaa tagatattcc gacattaatt ggcggctcag
     1321 cagatcttaa tgaatccaat atgacgcata ttaagtcgtc tacatccttt tctgcagaag
     1381 atagaaaagg taagaacata cattttggaa ttcgtgaaca tgctatgggt tcaatattaa
     1441 atggaatggc tatctatgga ggtataattc cttttggtgc aacattctta atattttcag
     1501 attatatgcg tcctgcaatt cggcttgcag cgttgatgaa acaaaaagtt atttatgttt
     1561 ttactcacga tagtattggt cttggggaag atggaccaac acatcagcca gttgagcaaa
     1621 tcgcctcgct tcgtgccatt cctaatttaa cggtcattcg tcctgcagat gcaaatgaaa
     1681 cagttgaagc ctggaagtat gcaatcaatc ataaaggcgg cccggttgct ttgattttaa
     1741 caagacaaaa actttctata attgatcaga ataaatatgg ttctgcaaaa gggctacaaa
     1801 acggagctta tatattaaaa gattcgaata agaatcctga tttactttta attgctacag
     1861 ggtcagaggt ttcattatcc ttaaaagcag cggatcagct tgaagcagaa ggaaaagttg
     1921 ttcgtgtaat aagttttcct agctgggaaa tttttgaaca gcaatccgat gaatacaaaa
     1981 agaaaatctt tcctgcagag attaaagcaa gagtttctgt tgagatggga gttggaatgg
     2041 gctggcaaaa atatgtcggt gattccggtg aagcaattag tatagaaaga tttggtgctt
     2101 ctgctccgga tagtattttg tatgagaaat atggatttac ggttgagaat gttgtggcga
     2161 cctcaaaaag agttttaagc aaaactaaat aattgattat gagcaagcta agaaaagatt
     2221 ttactcagaa agaatggaaa gattgctgcg gaagtttttg caaggattgt aaaattgcaa
     2281 atgcatatcg tgataaattt ggaaagaaag aaggggagaa aaaatttaat aaggatagaa
     2341 ag
//
LOCUS       DASWBY010000605         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_286449, whole genome shotgun sequence.
ACCESSION   DASWBY010000605 DASWBY010000000
VERSION     DASWBY010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286449"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1603
                     /locus_tag="VF870_11040"
     CDS             <1..>1603
                     /locus_tag="VF870_11040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9252768.1"
                     /translation="NQTSRINGYWKADFNDPIENPAQYLAIKNLHNNLTINIDEPNED
                     WYNIPGERLHFQNDSLHFERFWGLEKYDGIILPGDSVIRGVKLIKNKQLIPFTLRKIA
                     NINPNFKVPRSDLKGEPVLKYIYNKPVDYSDHISCGTLEEVGIDSVKIYDLVNRILTR
                     QIPNIHSLLILKDSKLVLEEYFYGYSAGKTHRVHSVTKSITSALFGIAVDKNSGLSID
                     EPVWKYFTDWDKSKWIMEKYDIKIEHLLSMTAGLDWKGLTLNESNDDIDMYNTDNYFG
                     YLLNRNLRFQPGSNFCYNNGLSLMLGHIIEKASGITADSFAAKYLFNHLKISNYSWDI
                     DKNGITRMDGGLKMRPRDMVRFGLLYLNDGIWNGEQILSENWIKNSTSQKINLNDRGY
                     AYHWWTMDYSIDGKIFRTYYALGHGEQAIIVVPGSKLVFVMTAGNYLQVEQRPFEIMS
                     QYILPSLKTNENSSNVDLAVFKGDYQINQTESINIDLIDNNLIATDPAGLKFRLIRKS
                     SLTFITDDQSRDIQFMTDDNAKVIGV"
BASE COUNT          555 a          263 c          313 g          472 t
ORIGIN      
        1 aaatcaaacc tccaggatta atggttattg gaaagctgat ttcaatgatc ccatcgaaaa
       61 tccggcgcaa tacctggcga ttaaaaattt acataataac ctgacaataa atattgatga
      121 acccaatgaa gattggtata acattcccgg tgaaagactg cattttcaaa atgatagtct
      181 ccattttgaa agattctggg gtttggaaaa gtatgatggt attattcttc ctggagattc
      241 tgtaatcagg ggagtaaagc taattaaaaa caaacaactt attcctttca ccctcaggaa
      301 aatagcaaat ataaatccta atttcaaagt gcccagatct gatctgaagg gtgaacccgt
      361 cctaaaatat atatacaata aaccagttga ttactcagat cacatttcgt gtggaacgct
      421 tgaagaagtc ggaattgatt ctgttaaaat ttatgatctt gtaaatagga tccttaccag
      481 gcagattcca aatatacata gcttattgat tctaaaggat agcaaacttg tacttgagga
      541 atacttttat ggttactctg caggtaagac ccaccgagtt cattctgtta caaaaagcat
      601 tacctcagcg ctgttcggga ttgcagttga taagaattca gggttatcta ttgacgaacc
      661 agtttggaaa tattttactg attgggacaa gtcaaagtgg attatggaaa agtacgacat
      721 aaagattgaa catttgttaa gtatgactgc aggcctggat tggaaaggct tgacgttaaa
      781 tgaatccaat gatgacattg acatgtacaa tacagataac tattttggat accttttaaa
      841 tagaaatctg agatttcaac caggctcaaa tttttgttat aataacggac tttccctgat
      901 gcttggacac atcattgaga aagcttcagg gataacagca gacagctttg ctgcaaaata
      961 tctttttaac catctgaaaa tcagcaacta ttcctgggat attgacaaaa atggtattac
     1021 aagaatggac ggaggattaa aaatgcgacc aagagatatg gtaagatttg gtctgttata
     1081 tttaaacgat ggtatatgga atggagaaca gatcctttca gaaaactgga ttaaaaattc
     1141 gacatcgcag aagattaatc ttaatgaccg gggctatgca taccattggt ggacaatgga
     1201 ttacagtata gatggaaaga tattcagaac ttattatgct ttgggacatg gagaacaggc
     1261 aataattgtt gttcccggat caaaattagt ttttgtgatg actgcaggca attacctgca
     1321 ggttgaacaa agaccatttg agattatgtc gcaatacatt ttaccttcct tgaagaccaa
     1381 cgagaactca agtaatgttg atcttgcagt tttcaaggga gattatcaaa ttaatcaaac
     1441 agaatccata aatatcgacc tgattgataa taatttaatt gcaactgatc ctgcaggtct
     1501 gaagttcaga ctaatacgga aatcgagttt aactttcatc acagatgatc aatcccgtga
     1561 tatacaattc atgacagatg ataacgcaaa ggttattggc gtc
//
LOCUS       DASWBY010000606         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_98499, whole genome shotgun sequence.
ACCESSION   DASWBY010000606 DASWBY010000000
VERSION     DASWBY010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98499"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1129
                     /locus_tag="VF870_11045"
     CDS             227..1129
                     /locus_tag="VF870_11045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX9252769.1"
                     /translation="MKKKIVIAGGTGFIGQYLERKFVDLGYKVKIISRQPHHISWSNQ
                     AGIIEALDQAEMVINLAGKSVNCRYTDKNKEEIFQSRTKTTKILGIALLACKNPPPLW
                     INSSTATIYRHAEDRPMTESSGEIGSGFSVDVAKVWEQSLFDFNLPQTRQVALRIAIV
                     LGADGGAMEPYINLVRFGLGGVQGSGKQMFSWIHIEDLYQIILFLQDNKDLSGVFNCS
                     APHPITNREFMGTLRNTMNRKFGLPSPKWMLEFGAVFMRTETELILKSRWVLPERLDK
                     EGFHFTFDSIGKALQQIVGNPSKI"
     gene            complement(1201..>2142)
                     /locus_tag="VF870_11050"
     CDS             complement(1201..>2142)
                     /locus_tag="VF870_11050"
                     /inference="COORDINATES: protein motif:HMM:NF017487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HEX9252770.1"
                     /translation="PFTKLVLRIFYAVGERLVETSMSPLTFFCNPIDATYGGTFRLKF
                     KAFFWISLQNQEVRGHTDMKNLLQFAYLQALSCLFPLIIFAALALTKFVDLPFLPRYD
                     FILLICIGAQIFMLASKLETIDEFKVICLFHVIGLVLELFKVSMGSWSYPEDAFSKIF
                     GVPLYSGFMYASVASYICQSWRRLDLQIYDWPKPFLAISISFLIYLNFFTHHFLFDAR
                     WVLKGLLFFIFLKTIVTYQLNGTRYKMPMILSFLLIGFFIWIAENITTFFGAWQYPNQ
                     ETAWSIVHLGKISSWFLLVIISIIIVAQLKRVKSLSR"
BASE COUNT          744 a          366 c          433 g          599 t
ORIGIN      
        1 ttgaacaagt ttattttaga tttttaccta ctttttacat atattagatt ttattaacat
       61 ctttctaaac tactattaag gtcttttaac caatagaaaa gccagcttca aaagtgaatt
      121 aaagtgccga agaatatcca gttagaagaa acgagtaagg aaattgcatc taatacttga
      181 ttaacatcgt cttgttaagt acaacgacaa gaaacgggtg aaagtgatga agaagaagat
      241 tgtcatagct ggaggtacgg gttttattgg acaatactta gagagaaaat ttgtggattt
      301 aggatataaa gtgaaaatca tttcaagaca accgcatcat atatcgtggt ctaatcaggc
      361 agggataata gaggctttgg atcaagctga gatggtcatt aatctagcgg gaaaatctgt
      421 aaactgtcgt tataccgaca aaaataaaga agagattttt cagtcaagaa caaagacaac
      481 taaaatcctc ggaattgcgc tattagcttg taaaaatcct ccgccgttat ggattaattc
      541 aagtacagca acgatttata ggcatgcaga agaccgaccc atgaccgaat cgagtggaga
      601 aattggctca ggtttctctg tggatgtagc gaaagtctgg gaacaatccc tctttgattt
      661 taatttacct caaacaaggc aggttgcttt gcgaattgcc atcgtgctcg gtgcggatgg
      721 tggcgcgatg gagccttaca tcaacttagt acgttttgga ctcggaggtg ttcaaggttc
      781 aggtaaacaa atgtttagtt ggattcatat tgaagatcta taccaaatta tattgttcct
      841 tcaagacaac aaagatttga gtggggtatt caattgttca gctcctcatc caatcacgaa
      901 tcgtgagttt atggggacat tgagaaacac catgaatcga aagtttggtc tgccttcacc
      961 taaatggatg ctagaatttg gtgctgtatt tatgcgaact gaaacggaat tgatattgaa
     1021 aagccgctgg gttcttcctg agcgcttaga taaagaaggg ttccatttta cgtttgattc
     1081 gattgggaaa gctcttcaac aaatagtggg aaatccaagt aaaatttgat gaatgactaa
     1141 aacacctcca ggaaaacgtg ttttcctgga ggtgttcttt atagattctt actttcttaa
     1201 ctatctcgag agactcttca ctctcttcaa ttgggcaacg ataataatgc taataatgac
     1261 aagtagaaac caggaactaa ttttccctaa atgaacgata gaccatgcag tttcttgatt
     1321 tgggtattgc catgcaccaa agaacgtcgt aatattttcg gcgatccaaa taaagaatcc
     1381 aatcaacagg aatgaaagaa tcattggcat tttataacgt gttccattca attgataagt
     1441 aacaattgtt ttgagaaaaa tgaaaaacaa caaacctttt aacacccacc gtgcatcgaa
     1501 tagaaaatgg tgggtaaaga aatttaaata gatcagaaag ctgatactga tggctaagaa
     1561 gggcttaggc caatcataga tttgtaaatc taaacggcgc caactttggc aaatatagct
     1621 cgccacactc gcatacataa agccactata aaggggaaca ccgaaaattt tactgaacgc
     1681 gtcctctgga taagaccaag accccataga tactttaaaa agttcaagga ctaaaccaat
     1741 gacgtgaaac agacaaataa ctttgaactc atcaatcgtt tctaatttgg atgcgagcat
     1801 gaaaatttga gcaccgatgc aaatgagtaa aataaagtca tatcgcggta gaaacggaag
     1861 atccacaaat ttagttaaag caagagcagc aaaaataatg agcggaaata aacatgagag
     1921 agcttgtaag taggcaaatt gcagaagatt tttcatgtca gtatgacccc gtacttcttg
     1981 gttttgtaat gaaatccaaa aaaatgcttt gaattttaga cgaaatgtac caccataagt
     2041 tgcgtctata ggattacaaa aaaaggttaa cggcgacatg gatgtttcaa caagtcgttc
     2101 cccaactgca tagaatattc ttaataccaa ctttgtaaag gg
//
LOCUS       DASWBY010000607         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_438744, whole genome shotgun sequence.
ACCESSION   DASWBY010000607 DASWBY010000000
VERSION     DASWBY010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438744"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VF870_11055"
     CDS             complement(<1..947)
                     /locus_tag="VF870_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9252771.1"
                     /translation="MFKFADPEYLNALWLIPVLIVLYILFNRNRKRLLEKFADKELHK
                     SIMYSFSGNKSKVKFGLILASFTLLILAFANPQVGTKMQEVKQTGIDVYILLDVSRSM
                     AAEDIKPNRLEKAKYQISNLIQKLRGDRIGLIIFSGDAYIQFPLTTDYSAANLFLSAV
                     DFSSVPQPGTAIASAINMAVQSFDSATTDKAIIVITDGEDHEGDIDKAVENATDKEIK
                     IYTIGLGSPEGVPIPVYDNRGKEVGFKQDNSRQTVLTRLNEPILKKIANDGNGKYYRG
                     NNYEDYLDKIYNDLSKLEQAEFGVKKVTDYEDRFYYFLLP"
     gene            complement(947..1942)
                     /locus_tag="VF870_11060"
     CDS             complement(947..1942)
                     /locus_tag="VF870_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9252772.1"
                     /translation="MFKDISFAYPWVLYFLLIIPALVAWYFWKGRKKQAAITYSSLKM
                     FEKIPATFRERLRHLPFALRMLALIFLIIALARPQNFSAGQSVNAEGIDIAMVLDISG
                     SMLAEDFKPNRLEAAKNVIDNFISARTTDRIGLVIFSREAFTQCPLTIDYAVLRNLLG
                     SIKTGMIEDGTAIGNGIANGINRLKDSGAESRVIILLTDGVNNAGEVDPISAAEIAST
                     FGIRIYTIGVGTRGEAPYPVQTPFGTRYQMMPVEIDEAILKKIADITGGEYFRATNNS
                     ALEEIYNKIDKMEKTKIEITSYRNAKELFAGWLGIGFALLLLELLASKTILRKLP"
     gene            complement(1935..2885)
                     /locus_tag="VF870_11065"
     CDS             complement(1935..2885)
                     /locus_tag="VF870_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252773.1"
                     /translation="MVKNIFLIWVLTFSINLFAQKISVTASTDTTDYMIGDHINYTLL
                     INLDKDVYIINPFFRDSLKNVDVIEIPDPKVEENENGKTVKYNYVLSRFDSAEVTIPP
                     IKIEYRTKSDSTLKYVLSNSVTFNVHRMNVSMKEEIKDIKPPIRLFNYLIIIYIAAAL
                     LLLAISYFIYRKYFKNKPQPVIKPRTEKISQHQLTLRKLNQLEKEELWQKGYVKEYHS
                     KITDIIREYFENQFELPLLERTTTESLKLLSKHPLGIKVLDITAKFLSNADLVKFAKF
                     TPLEIVNREMMTQAKEIVNKTISVQKEVEVEVETKEAANV"
     gene            complement(2879..3754)
                     /locus_tag="VF870_11070"
     CDS             complement(2879..3754)
                     /locus_tag="VF870_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9252774.1"
                     /translation="MLTKELLKQVRQIEIRTKGLVNQVFSGEYHSVFKGRGMEFSEVR
                     EYQFGDDIRNIDWNVTARFGHPYIKVFEEERELTVMLMVDLSGSLMFGSVAKTKQRIA
                     AELSAILAFSALKNNDKVGLILFTDKIEKFVPPRKGRKHVLRIIREVLSFEPEGKSTN
                     LKGALEYMNNTIKKKSIAFLISDFMDEGYEKILRIVGRKHDLIGIVLDDRREKEIPNI
                     GLVKLADAETGLERWIDTGSKRVRSQMISDRIQREKLRNSIFVKSGLDRIEVTTGSNY
                     IQPLVQFFRRREKRW"
     gene            complement(3757..>4026)
                     /locus_tag="VF870_11075"
     CDS             complement(3757..>4026)
                     /locus_tag="VF870_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX9252775.1"
                     /translation="ATRNPKLFGLNKLSELISYGASPRATIKLAIGAKAMAFIKRRGY
                     VIPEDVRAICLDVLRHRVAVTYEAEAEEITSENVIQEILNKVVVP"
BASE COUNT         1222 a          783 c          613 g         1408 t
ORIGIN      
        1 ggcagtaaaa agtaataaaa tcgatcttcg taatcagtga cttttttaac accaaattcc
       61 gcctgctcaa gttttgataa atcattgtaa attttatcca gataatcttc ataattattt
      121 ccgcggtagt atttgccatt gccatcatta gcaatctttt ttaagattgg ctcattaagt
      181 ctggttaaaa ctgtttgcct ggaattatcc tgcttaaatc ctacttcctt accacgatta
      241 tcataaaccg gaatcggtac tccttcaggg gaacctaatc caattgtata aattttaatt
      301 tctttatcag tcgcattttc aacagcttta tcaatgtcac cttcgtgatc ttctccatct
      361 gtaattacaa taattgcttt atcagttgta gcggaatcaa aagactgcac cgccatgttt
      421 attgctgatg caatggctgt tccgggttgt ggaactgaac taaaatccac cgcagaaaga
      481 aaaagatttg cagcagaata atcagttgtt aagggaaatt ggatgtacgc atcaccggag
      541 aaaattatta atccaattct atctccgcgt aatttttgta taagatttga tatctgatac
      601 tttgcttttt ccaatctgtt aggtttgata tcttcagcgg ccatacttct ggaaacatca
      661 agcaaaatat aaacatcaat tcctgtttgc tttacttcct gcatctttgt tccaacctgc
      721 ggatttgcaa atgcaagtat caataaagta aatgatgcaa gaattaaacc aaattttact
      781 ttagatttat ttccgctgaa agaatacatg attgacttat gtaattcctt gtcagcaaat
      841 ttttcgagta atctttttct attcctgtta aacaaaatgt acagcacaat taaaacagga
      901 atcaaccaga gtgcattaag atattcaggg tcagcaaact taaacattac ggcagtttcc
      961 ttaatattgt tttagatgct aacaattcta gaagcaataa tgcaaatcca atcccaagcc
     1021 atcctgcaaa aagttctttt gcatttctat aggatgtaat ttcaattttg gttttttcca
     1081 ttttatcgat tttattgtaa atctcttcca atgcactatt gttcgtcgca cggaaatatt
     1141 ctccgccggt aatgtctgca atctttttca aaattgcttc atcaatctca acaggcatca
     1201 tttgatatct tgtaccaaaa ggagtttgaa caggataagg cgcttctcca cgcgttccaa
     1261 caccgatggt ataaattcta ataccaaatg ttgatgctat ctcagctgct gatatcggat
     1321 caacttctcc cgcattatta acaccatcag ttaataaaat gataaccctg ctttcagcgc
     1381 cgctgtcttt taatctatta attccgttgg caattccatt tcctattgct gttccgtctt
     1441 caatcatacc tgttttaatt gaacccagaa gatttcttag cactgcgtaa tcaatcgtca
     1501 gcggacattg agtaaatgct tctcgtgaaa aaataactaa accaattctg tcagttgttc
     1561 ttgcacttat aaaattatcg attacatttt tagcagcttc caaacgattg ggcttaaagt
     1621 cttcagcaag catacttcct gaaatatcaa gcaccattgc gatatcaata ccttctgcgt
     1681 ttacactttg tccggccgaa aaattttgtg ggcgagccag agctataatt agaaatatta
     1741 aagccagcat tctcaatgca aaaggaagat gccttaatct ttctctaaaa gttgccggaa
     1801 tcttttcaaa catttttaat gatgagtaag taattgcagc ctgcttcttt cttcctttcc
     1861 agaagtacca ggcaactaag gccggaataa taagaaggaa ataaagcacc cacgggtatg
     1921 caaatgaaat atctttaaac atttgcagcc tcttttgttt ctacttctac ttcaacttct
     1981 ttctgaacag aaattgtttt gttaacaatt tcttttgcct gtgtcatcat ttcacgattt
     2041 acaatttcaa gcggagtaaa ctttgcgaat tttacaagat cagcgttact taaaaattta
     2101 gctgtaatat ccagcacttt aattccaagt ggatgcttac tcagcagttt caatgactct
     2161 gttgttgttc gctcaagtaa aggtaattca aattgatttt caaaatactc ccggattata
     2221 tccgtaatct tggagtgata ctccttaaca tatccttttt gccatagttc ttctttttcg
     2281 agctgattga gtttccgtaa agtcaattga tgctgagaaa ttttttctgt tctaggttta
     2341 attacaggct gtggtttgtt cttaaaatat tttctgtaaa taaaataact gattgctaaa
     2401 agcaatagtg cagctgctat gtaaattata atgagatagt tgaataatcg gatcggtggt
     2461 ttaatatcct ttatctcttc tttcatagaa acattcatgc gatgaacgtt aaatgttaca
     2521 gaatttgaaa gtacatattt taaagttgaa tcggatttag tccggtattc aatttttatc
     2581 ggtggaattg taacttcagc agaatcaaat cttgataaca cataattata ttttactgtt
     2641 tttccatttt cgttttcttc aactttagga tcaggaattt ctatcacatc aacattttta
     2701 agtgaatctc tgaaaaaagg attaatgatg taaacatctt tatccaaatt tatcagaaga
     2761 gtataattga tgtgatctcc aatcatataa tcggtggtat ctgtagaggc agttacactt
     2821 atcttttgtg caaaaagatt aatagaaaaa gtaagaaccc aaatcaagaa aatattcttt
     2881 accatctttt ctcacgtctt ctaaaaaatt gcactaacgg ctgtatgtag tttgaacctg
     2941 tagttacttc tattctgtct aagccgcttt ttacaaatat tgagttacgc agtttttctc
     3001 tctgaatgcg atccgaaatc atttgggagc gaacccgttt actgccagta tcaatccagc
     3061 gttccaagcc tgtttccgca tctgcaagtt taactaatcc gatatttgga atttcttttt
     3121 cgcgtctgtc atcaagaacg attccgatta aatcatgttt tctgccgact attcttagaa
     3181 tcttctcata gccttcatcc ataaaatcag aaattaaaaa tgcgattgat tttttcttga
     3241 ttgtattatt catgtattcc aatgcacctt taagatttgt tgatttacct tcaggttcaa
     3301 aagaaagaac ttcacgaata attcttaaaa catgctttct gccttttctt gggggaacaa
     3361 atttttcgat cttatctgta aataaaatca atccaacttt atcgttgttt tttaatgcgg
     3421 aaaaggcaag aatcgcagat aattcagcag caattctttg tttagttttt gcaaccgaac
     3481 caaacattaa cgaaccgctc aaatcaacca tgagcataac agtcaattca cgttcttctt
     3541 caaatacttt tatgtatgga tgaccaaagc gtgctgtaac gttccaatcg atgtttctta
     3601 tatcatcgcc aaactgatac tcacgcacct cggaaaactc cattccacgt cctttaaaca
     3661 cagaatggta ttcacctgaa aagacctggt ttacaagccc tttagtccga atttcaattt
     3721 gtctaacttg ttttaatagt tctttagtaa gcatttttat ggaacaacaa ctttattcaa
     3781 tatttcctgg ataacatttt cactagttat ttcttcagct tctgcctcat aagtaacagc
     3841 aacacgatgc cttaaaacat ccagacagat tgctctaaca tcttcaggaa ttacatatcc
     3901 tcttctttta ataaatgcca ttgcctttgc accgattgca agttttattg ttgctcttgg
     3961 tgaagcaccg taactaatta gttcagacaa cttatttaaa ccaaatagtt ttggattacg
     4021 agttgc
//
LOCUS       DASWBY010000608         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_739887, whole genome shotgun sequence.
ACCESSION   DASWBY010000608 DASWBY010000000
VERSION     DASWBY010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739887"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VF870_11080"
     CDS             <1..631
                     /locus_tag="VF870_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9252776.1"
                     /translation="EKILGIAGNKKKSILYKFLEEERNNSEGFIPQYFELGFGKFNNS
                     KDDDGSEFFIDNVKVRGKIDRVDVKPDGNKFKIIDYKLSGKKPTKKDLETGLSLQLPL
                     YLFASKKLIEAEINKEYDPAAAIIYSLKLNNKEFGEKIIHISSSRTPGEDELLKSNEE
                     LIKICNDFIPAYVKNIVEGKFNLSTLEDRENKVCRFCDFKSICRIQEVV"
     gene            875..>2369
                     /locus_tag="VF870_11085"
     CDS             875..>2369
                     /locus_tag="VF870_11085"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9252777.1"
                     /translation="MKTLVSFLAILIAGFSFAQNATDECILAKTQHFNRLQKFADINY
                     PGDAKYDVKYYKLDVAVNHIAHTISGNVTCNAVTTEPNVSQIYYDLTNPLVVDSVKVN
                     GVATTSFSRGSNTLIINLGNTLNQGDQFSTIVYYHGTPGSSGFGSFEFSSQNGNPAIW
                     TLSEPYGAKDWWPVKDTPADKADSADFWITVSTTLTPASNGKLMGIIDNGNGTHTYKW
                     KSSYPIAQYLLSMAITNYAQYTNYYHYSPTDSMPIDHFLYPANLNSNIAQLNKTPGMI
                     EIYAEHFGEYPFINEKYGHAQFGWGGGMEHQTITSMGGFGDMLIAHELAHMWYGDKIT
                     CKDWHHIWLNEGFATYGEGVITEAWYGKTGYDNFIASEMASAKNAVGSIWVQDITQVW
                     EIFNGNRSYAKGGVVLHMLRGIVGDSVFYDIMRTYSADPNLAYGVAVTEDFQAVAENV
                     SGLDLDYFFQQWIYGENYPKYHSWYSKSLISGNTWNLSLLITQDANTN"
BASE COUNT          791 a          361 c          467 g          750 t
ORIGIN      
        1 agaaaaaatt cttggtattg caggaaacaa gaaaaaatct attttatata aattccttga
       61 agaggaaaga aataattcag aaggatttat tcctcagtat tttgaattgg gatttggaaa
      121 gtttaataat tcaaaagatg atgatggcag tgaatttttt atcgacaatg taaaagtaag
      181 aggtaaaatc gatagagttg atgttaagcc agacggtaac aaatttaaaa ttatagatta
      241 caaactcagt ggaaagaaac caaccaagaa agatctcgaa accggattat cattgcagct
      301 tccgttatat ctattcgctt ctaaaaaact aattgaagct gagataaata aagaatatga
      361 tccagccgct gctattatat actcgctgaa attaaataat aaagagtttg gtgaaaaaat
      421 tattcacata agtagttcaa gaactccagg tgaagatgaa ttattaaaat ccaatgaaga
      481 gctgataaaa atttgtaatg actttattcc ggcttacgtt aaaaacattg tagaaggtaa
      541 gtttaatctc tcaacactgg aagacagaga aaacaaagtt tgccgttttt gtgattttaa
      601 atcaatctgc agaatacaag aagttgtttg atagctgcct ttacaatatt accattaata
      661 aaatctgata ttacactgat ctgtcatccc gagcgtagtc gagggaggga gaataatttt
      721 cccttctttc taatgatttt atcgtgattt ctatcggact caaaatgata ggggcctaat
      781 attttagagg ctttaacacc caattttatg cttgctttga tttgttttat cattaatttt
      841 aaaaatagac tgttaaccat tatttaggat ttatatgaaa actcttgttt cttttttagc
      901 gattcttata gcaggtttta gttttgcaca gaatgctaca gatgaatgta tccttgcaaa
      961 gacacaacat tttaatcgat tgcaaaaatt tgcagatatt aattatcctg gcgatgccaa
     1021 gtacgacgtt aaatattaca aattagatgt ggctgttaat catattgctc acacaatttc
     1081 aggaaatgta acctgcaatg cagtcactac tgaaccgaat gtttctcaaa tatattatga
     1141 tcttactaat ccattggtcg ttgattctgt aaaagtaaat ggcgttgcaa ctacatcatt
     1201 ttcccgcgga tcaaatacat tgattatcaa cctaggcaat acgcttaatc aaggagatca
     1261 gttttcaact atagtttatt atcatggcac acctggctca agcggatttg gaagttttga
     1321 gtttagttca cagaatggta atcctgctat atggacgtta agcgaaccat acggagctaa
     1381 agattggtgg cctgtaaaag atactccggc tgataaagca gattcagcag atttttggat
     1441 tacggtaagt acaacattaa cacctgcatc caatgggaag ttgatgggga ttattgataa
     1501 tggaaacgga acacatactt acaaatggaa aagcagttat ccaatcgcac agtatcttct
     1561 ttcgatggca ataacaaact atgcgcagta cacaaattat tatcattaca gtccaacaga
     1621 ttcaatgccg atagatcatt ttctttatcc ggcaaattta aactcaaata ttgctcagtt
     1681 aaataaaact ccgggtatga ttgaaattta tgccgagcat tttggtgaat atccgtttat
     1741 aaatgaaaag tatggacacg cacagttcgg ctggggcgga ggaatggagc atcaaacaat
     1801 tacatctatg ggtggatttg gtgatatgct tattgcccac gaacttgctc atatgtggta
     1861 tggtgataaa atcacttgta aagactggca ccatatctgg ctgaatgaag gttttgcaac
     1921 ttatggtgaa ggtgttatca ctgaagcgtg gtatggtaaa acaggttatg ataattttat
     1981 tgcaagtgag atggcaagtg cgaaaaatgc agttggatcg atttgggttc aggatattac
     2041 tcaggtctgg gaaattttta acggcaacag gtcttatgct aaaggtggag tggttttgca
     2101 tatgttaaga gggatcgttg gagattctgt attctatgat attatgagaa cttattctgc
     2161 tgatccaaat ttggcttatg gtgttgcggt aacagaagat tttcaagcag tggcagaaaa
     2221 tgtttcaggt ttagatttgg attatttttt ccagcaatgg atttatggcg agaattatcc
     2281 aaagtatcat tcctggtatt cgaaaagctt aatcagcggt aacacctgga atttatcgct
     2341 tcttattacg caggatgcaa acacaaatc
//
LOCUS       DASWBY010000609         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_98607, whole genome shotgun sequence.
ACCESSION   DASWBY010000609 DASWBY010000000
VERSION     DASWBY010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98607"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1418)
                     /locus_tag="VF870_11090"
     CDS             complement(201..1418)
                     /locus_tag="VF870_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9252778.1"
                     /translation="MKVGSLIKYHRTKIKMTQSQLATGICSVPHLSKIESNSKEANAE
                     TIRLLLKRLDINIEDVVESEKKVFTLLHLLYQHIYYFEVEKAQKTFQKLDAYKEIIVF
                     TQHIYLYEIYKLRYFLFVKDFVNADDQMRLLLSQKQNISQYEKYLLSYFSAILLILRG
                     KYGEADEKISTLIQSKSSNNSFEGEIYYHLALVKGYMGESGHAIYYGKKAMDLFKDHF
                     NYKRMLHVHMSLAINYTQTKIYDEALVCYEHLIRNIEMLQEYDLLPHIYHNIGDLRHK
                     MGDYSIAIDYFKKSSAIFNKNSKDYLLCLYNIALSEFRLEKWMESRESFILINKEALK
                     MKVTHYHLYSSFYLLLLNNKKQQAMRFLEDKVIPFTVKTDEQTEYHQYFSNILAEYYK
                     TEGKYEKAVKFIL"
     gene            complement(1715..2005)
                     /locus_tag="VF870_11095"
     CDS             complement(1715..2005)
                     /locus_tag="VF870_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252779.1"
                     /translation="MSSHLALYCPFNSIFVDLSDASTYTRNFSTPLLFSGAALAPTAG
                     MARLAVSKTLIKIFFDLFILFAPFCQMYFGSESNLSQPTRFGYWENYRFLVI"
     gene            2028..2972
                     /locus_tag="VF870_11100"
     CDS             2028..2972
                     /locus_tag="VF870_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9252780.1"
                     /translation="MSEKQFSALQKNKNVIVTKVQEVTLDTDIRATQSQIQAAEYPTQ
                     QTPWGIKAIYNNSSQTTTSGGAGINIAVLDTGVFTGHYDLANTVEQCKDFTQVTVPVN
                     GACNDAHGHGTHVAGSALADGGSDGAGIYGVAPQADLWAYKVLGDNGSGYSDDIAAAI
                     RHTADQATATGTKTVINMSLGSAGNDSLISSAVTYAYNKGVLVVAAAGNEGPNQGSIG
                     YPGALVNAVAVAALENVQQNGTYRVADFSSRGYASTDGDYIIQQGDVEISAPGAAIYS
                     TWNNGGYNTISGTSMASPHVAGLAAKLWASNPSWSHTT"
BASE COUNT          995 a          561 c          550 g          911 t
ORIGIN      
        1 aaagccttta atcaattgat taaaggcttc tttaacgaaa actttattca tcccactgga
       61 ggatgaattg gtacgctatt tgtctcttcg agggtaacca ctagtgaatt tgtttttgct
      121 gcatcagcac tcatagcccc aatagatgaa aagctgaaaa ttaacgctac aataatgaat
      181 ttcttcaatt ttaacactcc ttataaaatg aatttaactg ctttttcata ttttccttca
      241 gttttgtaat actctgcaag aatgtttgag aagtattgat ggtattctgt ttgttcgtct
      301 gtcttcactg taaatgggat gactttatct tctaaaaatc tcattgcctg ttgtttttta
      361 ttgtttagaa gtaacaaata aaatgaagag taaagatgat agtgagtaac tttcattttc
      421 agagcttctt tatttattaa gatgaaactc tctctacttt ccatccattt ttctaatcta
      481 aattctgaca acgcaatatt gtacaaacag agcaagtagt ctttagaatt cttattgaaa
      541 atggctgagc ttttcttgaa ataatcaata gcaattgagt aatcgcccat cttatgtctt
      601 aaatctccga tgttgtggta aatgtgtgga agaagatcat actcttgaag catttcaata
      661 tttcgaatga gatgttcata acaaactaga gcctcatcat atatcttagt ttgcgtataa
      721 ttaatcgcta atgacatgtg aacatgtaac attcgtttgt aattaaagtg atctttaaat
      781 aagtccatgg ctttttttcc ataataaatt gcgtgtcctg attcgcccat ataccctttg
      841 acaagggcca gatggtaata gatttcacct tcgaacgaat tattactcga tttactctgg
      901 attaatgttg aaatcttttc atcagcctct ccatacttcc ccctcaaaat taacaatatc
      961 gcagagaaat aagaaagtaa atacttctca tattgggaaa tattttgctt ttgtgaaaga
     1021 agcaagcgca tttgatcgtc tgcattcaca aagtctttaa caaagagaaa atatcgtaat
     1081 ttatatattt catataagta gatatgttgg gtgaaaacga taatctcctt atatgcatct
     1141 agtttctgaa aggttttctg cgctttctca acttcgaaat agtaaatatg ttgatagagt
     1201 agatgtaata atgtaaaaac ctttttttca ctttccacta catcttctat attaatatca
     1261 agccttttta ataaaagtct tatggtttct gcgttggctt ccttactatt actttcaatt
     1321 ttacttaggt gtggaacaga acagatgcca gttgcaagct ggctctgagt catttttatt
     1381 ttagtgcgat ggtacttgat taatgagcct actttcacat aatccaccct ctaacaaaat
     1441 ttttcactaa aagttattaa aattagaatt ttcccaacta ttaaaatagt taaagcggcg
     1501 ttctattaaa atagcctata aacatacgtt ctagcgatga atcaattact tttcatctta
     1561 acttaattgt gtgaaatgtt tcctttcttt tgtgcgaatc ttctaaaaat agttttaaag
     1621 actctaaatg ataatcttat aggggatata ttgacgcatg tgaaacagct aaaataggga
     1681 attagcagtg ttaaataatt tacaaataat gtaattaaat aactaaaaaa cggtaatttt
     1741 cccaataacc aaatctagta ggctgtgata gatttgattc agaaccaaaa tacatttgac
     1801 aaaaaggagc aaataagatg aaaagatcga agaaaatctt aatgagtgtt ttgctaactg
     1861 ctagtctagc cattcctgct gttggagcaa gtgctgcacc agagaacaat aatggtgtgg
     1921 agaaattccg tgtatatgta gaggcatctg ataagtcaac aaaaatggaa ttaaaaggac
     1981 agtacaaagc aagatgggaa ctcacagaaa atggtttctc gactgaaatg agcgaaaaac
     2041 aatttagtgc tctccaaaaa aataaaaatg ttatcgtaac aaaagttcag gaagttacac
     2101 ttgatacaga cattcgtgca acacaaagcc aaattcaagc ggctgaatat ccaacacaac
     2161 aaacaccgtg gggcattaaa gcgatttata ataacagcag ccagacaaca acttctggtg
     2221 gggcaggcat caatattgca gtgcttgaca caggtgtttt cactggtcac tacgatcttg
     2281 caaacacagt cgagcaatgt aaagacttca cacaagtaac agtgccagta aacggtgcat
     2341 gtaacgatgc acatggacac ggtacgcacg tagcaggatc agctcttgct gacggaggaa
     2401 gcgacggcgc tggaatttac ggagttgctc cacaagctga cctttgggca tacaaagttt
     2461 tgggcgacaa cggctcaggc tactcggacg atatcgctgc agccatccgt cacacagcgg
     2521 atcaagcaac ggcaactggc acaaaaacag ttatcaacat gtcacttggt tctgcaggaa
     2581 atgacagctt gatttcaagc gcagtcacat atgcatacaa caaaggcgtt ctagttgtag
     2641 cggcagcagg aaacgaaggt ccaaaccaag gttcaattgg ctatccaggc gcactagtta
     2701 acgcggtggc agttgctgca ttagaaaatg ttcagcaaaa cggaacatac cgcgttgcag
     2761 acttctcttc tcgcggatac gcttcaactg atggcgacta tatcatccaa caaggtgacg
     2821 ttgaaatctc ggcaccaggt gcagcaattt actctacatg gaataacggt ggttacaaca
     2881 caattagcgg tacatcgatg gcttcaccac acgtagctgg acttgctgca aaactgtggg
     2941 catcaaaccc aagctggtca cacacaacat aacgcgcaaa ccttcaaaat aaagcaaaag
     3001 ctgtcgatat taaaggt
//
LOCUS       DASWBY010000610         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_661800, whole genome shotgun sequence.
ACCESSION   DASWBY010000610 DASWBY010000000
VERSION     DASWBY010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661800"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..741)
                     /locus_tag="VF870_11105"
     CDS             complement(229..741)
                     /locus_tag="VF870_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9252781.1"
                     /translation="MKKFSIYNLQLCVLCFFVFIFFTSITQETSAQEKNLNDSVIVTS
                     EIRASLNKLNEVLATKDLKTVMSIYDDSDDITVIGSDSGEVFIGRSQVQKFMKIIISM
                     PFVFSFVMEPVTISFDQNIAWVFVDGKMVHTRDNGKISKFPYRIMAVMVKRGNEWKWK
                     VFSGSIPRGE"
     gene            complement(1237..>1846)
                     /locus_tag="VF870_11110"
     CDS             complement(1237..>1846)
                     /locus_tag="VF870_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252782.1"
                     /translation="YAALSVAIVLTFPSPDYFIWLCWQSLAVVSTALWFRSKFIVIAN
                     FFIFLSIILAYLAIAGETSVSAVSFGIVALISARIMNWQKERLELKTENLRIAYLVIA
                     FLILPLVLYINLPSQLVGISFIALAFIYYVLGKIINNKKYRLMASGTLLLSIVYIFIF
                     GITSSDTTYKIFSFLLVSLALVIISIVYAKIRTKEKSDEAKA"
BASE COUNT          661 a          325 c          277 g          583 t
ORIGIN      
        1 catcatcatt gctcttacaa atcagtatca agaaaactga atccacgctc agagtaggga
       61 aaacagaaag tacatcaatc ctattccgac agaaggtata gatgtattta ttattaatat
      121 atattaataa taaatattta cttagcaacg ggcttcataa taagaactag tcaaaaacca
      181 cataaggtgt tagctaaata tgtgatgctg aactaaaggg tgtttagttt actcacctct
      241 aggaattgat cctgaaaaaa ctttccattt ccattcgttg ccccttttta ccatcacagc
      301 cataatgcga taagggaatt tagagatttt cccattgtct cttgtgtgaa ccatttttcc
      361 atcaacaaat acccaggcaa tattttgatc aaaactgatt gtaacgggct ccataacaaa
      421 tgaaaagaca aagggcattg aaataattat tttcataaac ttctgaactt gtgacctgcc
      481 aataaagacc tctcctgaat cggatccaat cactgtaata tcatctgagt catcatatat
      541 actcatcaca gtctttaaat cttttgttgc caaaacttcg ttcaatttat taagacttgc
      601 tctaatttct gaggtaacaa tgacagagtc attaaggttt ttttcttgtg cggaagtttc
      661 ttgagttata cttgtaaaaa agataaaaac gaaaaagcac aatacgcata attgaaggtt
      721 gtaaattgaa aattttttca tcttaattag tcctttgcag tttagcaata attcgagaat
      781 tagatcatta atttatttaa taagccgatc aataataaaa tcaagatttt ggttcgttta
      841 aagtagcttt aataatcaaa aacaagcctc agaatcacta cttaaaaagg gagacaaata
      901 atacttttac tttagcaaca tcatcttctt tatttctaca aaactaccag attgcaggtt
      961 gtaaaaataa attactgttg aaagcttaga ggcattccag ttaacttcac agcaacacgc
     1021 gagtttttta tgtgcataaa tgcttacaaa tggcagcgaa ttatccgatg cgccggatta
     1081 attaaaatct ataatcagag gaaatgagag aaaataggtc caatatattt atagattcct
     1141 cttcttatac ttttctctct ctctgaaata attaatccaa ctttttttta aaattctttc
     1201 aataataaaa atccacttta tacataaaaa cgggttttat gcttttgcct catcactttt
     1261 ttctttagtc cttattttag catagacaat tgatattatt acgagagcca agcttaccag
     1321 caggaaagaa aatattttat acgttgtgtc tgaactagtt attccgaata taaaaatgta
     1381 aacaatagac aggagcaacg tgccggaagc catcaatctg tactttttat tattgattat
     1441 tttaccgagc acataataaa taaatgccaa tgcgataaat gaaataccaa ctaactgaga
     1501 aggcagattt atatataaaa ctaacggcag tataaggaat gcaattacca ggtatgcaat
     1561 tcttaagttt tctgttttaa gttccagtct ttctttctgc caattcatga ttctggcact
     1621 tattagcgct acaataccaa agcttactgc acttacagat gtttctccgg caatagccag
     1681 ataagctaag ataatagaaa gaaaaatgaa aaaattagct ataactataa acttggatct
     1741 gaaccagagc gcagttgaaa ccacagcaag actctgccag cacagccaga taaaatagtc
     1801 aggtgatgga aaggttaaaa ctatagctac gctaagcgct gcataa
//
LOCUS       DASWBY010000611         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_98653, whole genome shotgun sequence.
ACCESSION   DASWBY010000611 DASWBY010000000
VERSION     DASWBY010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98653"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VF870_11115"
     CDS             <1..655
                     /locus_tag="VF870_11115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9252783.1"
                     /translation="ILNLLACKVAIPFLDSKNIFPIEISYFLSVGLLVLAPMFLGAIY
                     LTSKEINSSKLKDILTRMRIENLSKTDVVWSIGGFILLSFLSFLIASFVLPVFDINAN
                     PFFFQNMPLDENHFWIIYVWPLFFFFNIFGEEFYWRGYIQPRQELLNKKFTWLIHGLL
                     WAFWHLPMGLNLIYSAVPILFILPGIVQIRKNTTISIIIHFVFGAFGFLVLAFGLVH"
     gene            complement(723..917)
                     /locus_tag="VF870_11120"
     CDS             complement(723..917)
                     /locus_tag="VF870_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HEX9252784.1"
                     /translation="MNGNKRSDIIIGMQVKIVLKEDQRTGELTEGIVKDILTKSSKHP
                     HGIKVRLETGEVGRVKEIVE"
     gene            1109..>1677
                     /locus_tag="VF870_11125"
     CDS             1109..>1677
                     /locus_tag="VF870_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX9252785.1"
                     /translation="MKLIFKKYYSKLSLNTSVLLDRAKMTEHTFMIVVAIIIGVLAGF
                     SAIGIRAMIKFFSQLSFPGPGNLLENIISTPWYLIIIIPVIGGIIVGPLIYYFAPEAK
                     GHGVPEVMQAILLKGGQIRGRVAIIKALASAITIGTGGSVGREGPIIQIGSSLGSVVG
                     QFLRVSPKRLKTLVGCGAAAGIAAAFNAP"
BASE COUNT          486 a          291 c          292 g          608 t
ORIGIN      
        1 tattttaaat ttgctcgcgt gcaaagttgc aatacctttt ctggattcaa aaaatatttt
       61 cccgatagag atttcatatt ttttaagtgt cggattactt gtactggcac ctatgttttt
      121 gggtgcaatc tatctaactt ctaaagaaat taattccagt aagctcaaag atattctgac
      181 caggatgagg atagaaaatc tttccaaaac agatgttgtc tggagtattg ggggatttat
      241 tttactttca tttctttcat ttttaattgc aagctttgtc cttccggttt ttgatattaa
      301 tgcaaaccct ttcttttttc agaatatgcc tttagatgaa aatcatttct ggataattta
      361 tgtctggcca ttgtttttct ttttcaacat ttttggtgaa gaattttact ggagaggtta
      421 cattcagcca agacaggaac tgcttaataa gaaatttaca tggttgatac acggattgct
      481 ctgggcattc tggcatttac caatgggatt gaatttgatt tattctgctg tacctatatt
      541 atttatactt ccgggaattg ttcagattag aaaaaacaca accatttcta taattattca
      601 ttttgtgttt ggagcattcg gatttttagt cttggctttt ggactggtac attaaaataa
      661 ttaagattaa ataaccataa aacgaaacat catccaaagc gtgaacgtgt cagcctggaa
      721 aattattcca ctatttcttt aacacgtcca acttctcctg tttctaatcg tacctttatc
      781 ccatgtggat gcttcgaaga ttttgtaaga atgtctttaa caataccctc cgtaagttcg
      841 cccgttcgct ggtcctcttt caatactatt ttaacctgca tacctattat aatatcagat
      901 cgtttatttc cattcattat tcagaatcct ttttgtcata tatagttaaa aatttcggat
      961 acagtttata tctattatta agaaatttta tactctagac aataccaagc ataatattac
     1021 aaaatatttg atcaagacat tcaacagtct tatttttatt caacgcttca atttcagtta
     1081 atatcgatat gcgcaatttg gtgaaatcgt gaaattaatt ttcaaaaaat actattctaa
     1141 attatcactt aacacttccg ttcttttaga cagggctaag atgactgagc acacttttat
     1201 gattgtcgtt gcaattataa ttggtgtact tgctggtttt tccgcaatcg gcattcgtgc
     1261 aatgataaaa tttttttctc agctttcctt tccaggtcca ggaaatctgc tggaaaatat
     1321 aattagtact ccctggtatc ttatcattat aataccagtt attggcggaa taattgttgg
     1381 accgctgatt tactattttg cacccgaagc aaagggacat ggtgttccgg aagtaatgca
     1441 ggccatttta ttaaagggag gtcagataag gggaagagtt gccattatta aagcacttgc
     1501 atctgcaata acaattggta ccggtggatc agttggaaga gaaggaccaa ttatacagat
     1561 aggttcaagt cttggttccg tcgttggaca gtttctgaga gtttcaccca agcgattgaa
     1621 aactttagtt ggatgcggcg ctgctgctgg aattgctgca gcattcaatg cgccaat
//
LOCUS       DASWBY010000612         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_395553, whole genome shotgun sequence.
ACCESSION   DASWBY010000612 DASWBY010000000
VERSION     DASWBY010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395553"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VF870_11130"
     CDS             complement(<1..723)
                     /locus_tag="VF870_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9252786.1"
                     /translation="MNTIEIKNIVKTYPAKKNKITAVDDVSFSVKEGELFGLIGPDGS
                     GKSSIFRILTTLLLADSGRASVMGMDVVNDYKEIRKIIGYMPGRFSLYQDLTVEENLE
                     FFATIFNTTIQANYNLIADIYVQLEPFKKRRAGKLSGGMKQKLALCCALIHKPKVLFL
                     DEPTTGVDPVSRKEFWEMLKRLKEQGISILVSTPYMDEAKLCDRIALIQNGKLLEIDT
                     PQNIVNRFDSTLYAVRSENMFKL"
     gene            complement(725..1624)
                     /locus_tag="VF870_11135"
     CDS             complement(725..1624)
                     /locus_tag="VF870_11135"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX9252787.1"
                     /translation="MKSILLKIVLILFTGLLISCSNKNGKFDATGTFESEEVIVSSEA
                     MGKLLKFEVEEGMTLKKDQIVGLVDTTQLYLKKKQLQSTIKSVLSKQPDIPTQLAAIK
                     EQIATAEREKNRIENLVSSDAATTKQLDDINSQLEVLNKQYDATKSSLTITKEGIQSE
                     TLPLIAQVEQIQDQINKSIIKNPIDGTVLTRYAKQDEITSSGKALYKIADLSEMILRA
                     YINGDQLGQVKLDQKVKVYVDKGNGKQKELEGEVYWVSSKAEFTPKTIQTKDERANLV
                     YAIKVRVKNDGYIKIGMYGEVKF"
     gene            complement(1645..2907)
                     /locus_tag="VF870_11140"
     CDS             complement(1645..2907)
                     /locus_tag="VF870_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9252788.1"
                     /translation="MKRLLFVILLIVSQNIIIPQTQLTLEDCYEKARINYPLIKQKEY
                     IEKTKDYSVSNVWNGYFPQITILGQATYQSDVTQVPMPLPGIVIQRLSKDQYKVVANV
                     TQTIYDGGVMSSQAGIQESANEIDNQKIEIELLNLKERVNQIYLGILLIDAQLNQIEL
                     VKNDLNESISKLEAAYLNGTATKSDVDVLKAELLKSEQQKIQLHSSRISYLDMLGLLI
                     NENLDESTTLTTPSQINFLSATEIIRPELKLYSAQKNLIENQNGITTSKIIPKASLFF
                     QGGYGKPGLNMFVNDFAWYYITGIQFSWSLSNLYSYGNESEINQLSLQSIDAQTETFL
                     LNTKITTNQQLQEIDKLNKLIEVDKGIIELRTSVKLAAKSKLENGVITSNDYIRELNA
                     EDTVKQNLEIHKIQLLLAQYNYKITTGN"
     gene            complement(2910..>3482)
                     /locus_tag="VF870_11145"
     CDS             complement(2910..>3482)
                     /locus_tag="VF870_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9252789.1"
                     /translation="TEEKFFRDGFYKTTMDEVASELKMSKKTIYKFFPSKEDLITAIA
                     KHFMEGMKSKIVPALNSDKNAIEKLAELNNILAKASEKISSNRMDEIRKHFPSLWNEI
                     DSFRTKMMFGNITKVIDQGKSEGLFIDYPTPIIMNTLVSSVRAIVNPEFILNNNFSIG
                     EAASYAFKVIIGGIVTENGRKIFNKTINKI"
BASE COUNT          978 a          616 c          545 g         1343 t
ORIGIN      
        1 gagcttaaac atattttcag agcgaacagc ataaagagtg ctatcaaatc tgtttacaat
       61 attttgaggc gtatctattt ctaaaagttt gccattttga ataagtgcaa ttctatcaca
      121 aagttttgct tcatccatat atggcgttga tacaagaata cttattcctt gctctttcaa
      181 tctttttagc atttcccaaa attcttttct ggatacaggg tcaacaccgg ttgttggttc
      241 gtcaagaaaa agaacttttg gtttgtgaat tagtgcacag caaagggcaa gtttctgttt
      301 cattccaccg ctaagttttc cggctcggcg ttttttaaaa ggttcaagct gtacataaat
      361 gtcagcaatt aaattgtagt tagcttggat ggtggtatta aagattgttg caaaaaattc
      421 taaattttct tcaacggtca gatcctggta aagtgaaaac ctgccgggca tatagccaat
      481 aattttcctg atttctttat aatcatttac aacatccatc cccataactg aagctcttcc
      541 gctgtctgca agaagtaagg ttgttaaaat tctaaagatt gatgatttgc ctgaaccatc
      601 cggaccaatc aaaccaaaca gctcaccttc ttttacagaa aatgagacat catcaactgc
      661 tgtaatctta ttttttttag ctggatatgt tttgacaata ttttttattt caatcgtatt
      721 catatcaaaa ttttacttca ccgtacattc cgatttttat ataaccatca ttcttaaccc
      781 gaaccttgat tgcgtaaact aggtttgcac gttcatcttt agtttgaatt gtttttggag
      841 taaactcagc ttttgaagaa acccaataaa cttctccttc cagttctttc tgtttaccat
      901 tacctttatc tacataaact tttacttttt gatcaagttt aacctggcct aactgatcgc
      961 cattgatata agcacgcaaa atcatttctg ataaatctgc aattttataa agtgcttttc
     1021 cgcttgaagt tatttcatcc tgttttgcgt agcgcgttaa aactgttcca tcaattggat
     1081 tttttataat gcttttatta atctgatcct gtatctgttc aacttgtgca atcagaggaa
     1141 gggtttcact ctgaattcct tctttggtaa ttgttaaact agatttcgtt gcatcgtact
     1201 gcttattcaa aacctcaagt tgcgaattga tatcatccaa ctgttttgtt gttgctgcat
     1261 cagagcttac aagattttca attctgtttt tttctctttc ggcagtcgct atttgttcct
     1321 ttatagctgc aagctgtgtt ggaatatccg gctgcttgct taacactgat ttaattgttg
     1381 attggagttg ttttttctta agatataact gagttgtatc aaccaatcct acgatctgat
     1441 ctttttttag agtcatacct tcttcaactt cgaactttaa aagttttccc atcgcctctg
     1501 aagaaactat tacttcttcg gattcaaatg ttcctgtagc atcaaacttt ccattcttgt
     1561 ttgagcaaga aattaacaac cctgtaaaaa gaataagaac aatttttaat aatatacttt
     1621 tcattttcat ctctattata atttttaatt tcctgttgtg attttgtaat tgtactgtgc
     1681 cagcaataac tgaattttgt gaatttctaa attttgcttc acggtatcct ctgcattcag
     1741 ttcacggata tagtcgtttg aggtaataac tccgttttca agctttgatt ttgcagctaa
     1801 cttaactgaa gttcttagtt caataatccc tttatctacc tcaatcaatt tgtttaactt
     1861 atcaatttct tgcagctgct ggtttgtagt gatttttgta tttaataaaa atgtttctgt
     1921 ttgcgcatca atgctttgca ggcttaattg gttgatttca ctttcatttc cgtagctgta
     1981 aagatttgat aaagaccatg aaaattgaat acctgttatg tagtaccacg caaaatcatt
     2041 tacaaacata tttaaaccag gtttaccata accgccctga aagaagaggc tggctttggg
     2101 aataatttta gaagtagtta taccattttg gttttcaatc agattttttt gagccgagta
     2161 aagttttaat tcgggccgaa tgatttcagt tgccgataga aagtttattt gtgatggagt
     2221 tgttagtgtg gttgattcat ctaaattttc atttatcagc aatccaagca tatctaagta
     2281 agatattcgt gaggagtgta attgaatttt ttgttgttca gattttaaaa gctcagcctt
     2341 taaaacgtca acatctgatt ttgttgcggt gccatttaaa tatgcagcct caagttttga
     2401 aatgctttca ttcaaatcgt tttttacaag ttcaatttga tttaattgag catctatcag
     2461 gagtattcct aaataaatct ggttaactct ctcttttaga tttaataatt ctatttcaat
     2521 cttctgattg tcaatctcat tagcagattc ctgaattcct gcttgtgaac tcattactcc
     2581 gccgtcataa atagtttgag taacattagc tacaacttta tattgatctt ttgagagtct
     2641 ttgaattaca atccctggta aaggcattgg aacctgtgtt acatcagatt gataagtagc
     2701 ttgtcccaga attgttatct gcggaaaata tccgttccag acattgctta cactgtaatc
     2761 tttggttttt tcaatatatt ctttctgctt gataagtgga tagtttatcc ttgctttctc
     2821 ataacaatcc tcgagcgtaa gctgtgtttg aggaataata atattctgac ttacaattag
     2881 caggattaca aataataatc ttttcatagt taaatcttat ttattgtttt gttaaaaatt
     2941 tttcttccat tttctgtaac aattcctccg ataattactt tgaaagcgta cgacgcggct
     3001 tcaccaatag aaaaattatt attcagtatg aattcaggat ttacaattgc tcttacggat
     3061 gaaaccagtg tgttcattat tattggagta ggataatcaa taaacaaacc ttctgatttt
     3121 ccctgatcaa ttacttttgt aatatttcca aacatcatct ttgttctgaa gctatctatt
     3181 tcattccaaa gtgaagggaa gtgctttctt atctcatcca ttctatttga agaaattttt
     3241 tctgatgctt tagccaaaat gttattgagt tccgccagtt tttcaattgc atttttatca
     3301 gagttcaacg ctgggactat cttgctcttc attccttcca taaaatgttt tgcaattgca
     3361 gtaatgaggt cttctttaga agggaagaat ttatagatgg ttttcttgct catttttaat
     3421 tctgaggcaa cttcatccat agtggttttg taaaaaccat ctctgaagaa tttttcttca
     3481 gt
//
LOCUS       DASWBY010000613         9989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_98689, whole genome shotgun sequence.
ACCESSION   DASWBY010000613 DASWBY010000000
VERSION     DASWBY010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9989
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98689"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="VF870_11150"
     CDS             complement(<1..1640)
                     /locus_tag="VF870_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HEX9252790.1"
                     /translation="MIFLNPAILFGLLAASIPVIIHLFNLRKLKKIEFSTLTFLKELQ
                     KNKIRKIKLKQWILLALRVLIILFVVMAFARPTLQSIQIGGTTSAAKTTAIFILDDTF
                     SMSVVDQKGSYFNQAKEIIKQIVSQLQEGDEVGLILVSDNKNESQLSSNLSEFEQNVD
                     RLDLSFASGDLNSAITKAAQLISESKNFNKEIYVVSDFQKNKITKESLNNDLSELLNE
                     SIRLYSFDLSDKDVFNLSVDDLKINNQIFEKDKPVNFSVTITNDSKQNVNSAVVSLFL
                     NEERAAQKSFDVNAGQSTIVEIEATPKTTGFISVVAEIETDEIEQDNKRFASLYIPEK
                     TAVGLFSENSGDLTFVGLALQTAGEGKYEIERKNINQLTSQQLSKYQAIIISANVIPS
                     GIEQLKNYMQNGGGLILFPSSNPDVHKLNEFYSQLGLALRASFVGKVNSVNLKIKFDK
                     TDFNHPVFQNIFQNEEKKKYESPELNAYYKLTSAGNQIVSLIDGSPFLSEFKIGNGKA
                     FVFNSSPVLSWSDFPIKSIFAPLMNKSVAYLSSKEREQ"
     gene            1902..2372
                     /locus_tag="VF870_11155"
     CDS             1902..2372
                     /locus_tag="VF870_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252791.1"
                     /translation="MGEQLKKWLKQTQFESLDQEVALNIFVTSYFLRSKHDEVMGNYG
                     LTMPQYNVLRILKGVYPDGYPRCEIITRMIEPAPDVTRIIDRLLKDKLVERFNSKEDK
                     RLSMTRITDKGIKLLEKSNPEVQALGSYISSTLKNSEKKDLSDLLEKVYGSVVK"
     gene            complement(2660..4918)
                     /locus_tag="VF870_11160"
     CDS             complement(2660..4918)
                     /locus_tag="VF870_11160"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX9252792.1"
                     /translation="MTANYDMIKKVYANYRTKLTEVRKVVNRPLTYAEKVLYTHLYDK
                     ATKEFSGGKDYVDLAPDRVAMQDATAQMALLQFMSAGRKTTAVPSTVHCDHLIQAQVG
                     AVEDLNRATTENKEVYDFLESISQKFGVGFWKPGAGIIHQVVLENYAFPGGMMIGTDS
                     HTPNAGGLGMIAIGVGGADAVDVMAGMPWELKWPKLIGVKLTGEMNGWTSAKDVILKL
                     AGILTVKGGTGAIVEYFGDGTNSISCTGKGTICNMGAEIGATTSVFPFDERMSSYLRI
                     TKRVDVAALAEGLADELCADKEVFDNPEKYYDQVIEINLSELEPHLNGPFTPDKAWPI
                     SKMKEAVKSENYTDQISGGLIGSCTNSSYEDIDRSASIAKQAITKGLKTKSEFTITPG
                     SEQVRATIERDGQLKTLTDIGGVILANACGPCIGMWKRMDIKTGERNTIVTSFNRNFA
                     KRNDGNPETLAFVASPEITTALAIAGSLSFNPITDELVNEKGEKVKLDPPKGEELPPR
                     GFDEGSAGFVAPAEDGSKVEVKVDPKSDRLQLLTSFTPWDGKDLIDLPVLLKAFGKCT
                     TDHISMAGPWLKYRGHLDNISNNMFIGAINAYTKEAGKVKNAFSGETKSVPEVAREYK
                     AKGVNWVVVGDENYGEGSSREHAAMEPRFLGGRAIIVKSFARIHETNLKKQGMLPLTF
                     ADPKDYDKIREDDKVDLLISSLAPEKQVKMIIKHKDGSKDEVMLNHTFNAAQIEWFKA
                     GSALNLIASKQK"
     gene            5165..5659
                     /locus_tag="VF870_11165"
     CDS             5165..5659
                     /locus_tag="VF870_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252793.1"
                     /translation="MSNGVQSFLSLGAMMIFSLISLRFDTAVLQNTEVEVENKVYLTA
                     FSLADDLLEEIKQRAFDEKTVDFKAITTSALTPSANLGKDSGEEWPNFNDIDDYNDYT
                     KPVSLPHAEKYTVISKINYVLESDQDQISSDPTYFKRVTIFVESPYLKHQIKLSYIFT
                     LHSK"
     gene            5664..6248
                     /locus_tag="VF870_11170"
     CDS             5664..6248
                     /locus_tag="VF870_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252794.1"
                     /translation="MNVLIDLIGSSFIGGILLLLILKLNLYTQNTSISSDSELRIQQN
                     AKTLAEIINYDLRKVGYNYNNTAMITADSNKIKFYADMNEPGTAGYGTIDVVEYFLSS
                     PSKASSTTNPKDVVLTRVLNGTDTLSGPSLGLVKLVFSYYNKGLVTTTNLDSIKYIKT
                     EFWVESLEPIEVNFGTQTGYPFTYWEMTINPRNI"
     gene            6250..7290
                     /locus_tag="VF870_11175"
     CDS             6250..7290
                     /locus_tag="VF870_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252795.1"
                     /translation="MGKSILLITLGISVIISLFILKLNANSKEGLSVSVNMFDQNQAR
                     LIANSGVEVYLEKLKADKSLIDKTFTGNNLFGGSYDISIEGPDTLVKVTSFAKFQDVT
                     HKTIVFAKADKIPLIPGPGAMYLTANTISSFKKSGIGGSVLIDGNNHDINGNLLGDGI
                     VTPGIAVDGIDQRNDVINIIKSNAIDQVIGSGGSPSVATINNTTDWNEYALQLVENPD
                     IIIDTQDKIKTMNNWGTVADPKVTFVNGDISINNSETAEGCGILVVNGNLSINGNFTY
                     RGLIIAYKETTIEIKLNGNGKIYGGIVVAGQSIVIEVASGNFTTKYSMPAMNHVATLL
                     KTNRFQIVSWWE"
     gene            7449..7931
                     /locus_tag="VF870_11180"
     CDS             7449..7931
                     /locus_tag="VF870_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252796.1"
                     /translation="MSNIQSMLAIGSLFLLSMVSLNFNSALMQSNTAELENKVYLTAF
                     SLADDLIEEIKQKGYDEKTLDFPTVNRSTLTPALALGPESEVYPFFDDIDDYNNYSRD
                     ADAPHSEKYHISAKVYYVSETNPDTKSVTQTFHKRVDVTVSSPYLNNPVVLTFIFTHK
                     "
     gene            7933..8505
                     /locus_tag="VF870_11185"
     CDS             7933..8505
                     /locus_tag="VF870_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252797.1"
                     /translation="MASLIDILGSSFIGGLLLLLLLKLNLFANSSSVTSDNELKLLQN
                     AKTLAEIINYDFRKIGYEYDGTAIAIADSNHIKFYADIDRNNTIDTLEYYTSDTTVAT
                     GTENPRDIILFRKYNGVAMGGPTLGLTKIKLSYLNEFNVVTNVLANIKYIKVELWVES
                     TIPEMESNFVNPNHPYLFTYWEMKINPRNI"
     gene            8508..9533
                     /locus_tag="VF870_11190"
     CDS             8508..9533
                     /locus_tag="VF870_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252798.1"
                     /translation="MGKMSLILTMGIAVIIGFFVLRLNSNAKQGLSTTLDMYEYTQAR
                     LIANSGVEIYLEKMRRDKTINSGTYLNNDLADGSYDIYITGPDTALNIRSVAHFMNVT
                     HETIVKAKRDPIKFPTAPGALYVSTSAMSNVMMTGNFTVSGFNHDKNGTLVSTTNNTV
                     PGVVVDNNADSIAIVSILKKNTANNITGKGGGTPNVAVMPQGSDWAAISNDIAFSADK
                     TIGSGIYNTGEFGTCSNPQITLVNGDVEFNGSLTGAGILVVNGDLTITGTFKWKGLII
                     AYKESAITTKLNGTGTVIGSLIVSGNSVDMNISNGTFEALYSPEALNNAKLNLKSSRF
                     KILSWWE"
BASE COUNT         3342 a         1719 c         1665 g         3263 t
ORIGIN      
        1 ttttgttctc tttcttttga tgaaagatat gcaactgatt tgttcatcag tggagcaaaa
       61 atacttttaa tcggaaaatc actccagctt aaaacgggag aagagttaaa aacaaatgct
      121 tttccgtttc caattttaaa ttcacttaaa aatggtgaac catcaatcaa agaaactatt
      181 tgattgcctg cagatgttaa tttgtagtaa gcgttaagtt caggagattc atattttttc
      241 ttttcttcat tctgaaaaat attttgaaaa acaggatgat taaaatctgt tttatcaaat
      301 ttaattttca aattcacact gtttactttg ccaacgaatg atgctcttaa tgctaatccc
      361 aactgagaat aaaattcatt taatttatga acatctggat tcgatgaagg gaataaaatc
      421 aatccgccgc cattctgcat ataatttttt agctgctcaa ttccagaggg gataacatta
      481 gctgatataa taattgcctg gtatttactt aactgttgag atgtaagctg attaatattt
      541 tttctttcga tttcatactt cccttcccct gcagtttgta atgcgaggcc aacaaaagtt
      601 aaatcaccag aattttcaga aaacaatcca actgcagttt tttcagggat gtacaaactt
      661 gcaaatcgtt tattatcctg ttcaatttca tcggtttcta tttcagcaac aacactaata
      721 aatcctgtcg ttttgggagt tgcttcaatc tcaacaattg ttgattgtcc ggcattcaca
      781 tcaaaacttt tttgagctgc tctttcttca ttcaaaaaca atgatacaac agcgctgttt
      841 acattctgtt tagagtcgtt tgtaattgtt actgaaaaat taaccggttt atctttctca
      901 aaaatctgat tatttatttt taaatcatca acagaaagat taaaaacatc tttgtctgat
      961 aaatcaaaag agtacagtct gatactttca ttcagtaatt cactcagatc attattaagg
     1021 ctttctttgg tgattttatt tttttgaaaa tctgatacga cataaatctc tttgttaaaa
     1081 ttcttgcttt cagaaattaa ttgagcagct tttgtgattg ctgaatttaa atcaccggag
     1141 gcaaaagata aatcgagtcg atcaacattc tgttcaaatt cagataagtt tgatgaaagt
     1201 tgactttcat tcttattatc agaaactaaa atcaaaccca cttcatcacc ctcctgaagc
     1261 tgagagacaa tctgctttat gatctctttt gcctgattaa agtacgaacc tttctgatcc
     1321 acaactgaca tactaaatgt gtcatcaaga ataaaaatcg ctgtggtttt tgctgcagaa
     1381 gtagtccctc cgatttgaat ggactgcaaa gttggtcgtg caaaagccat cacaacaaaa
     1441 agaataatca acacacgaag tgcaagtaaa atccattgct ttaattttat ttttcggatt
     1501 ttatttttct gcagctcttt aagaaaagta agagtgctaa attcaatctt tttaagtttc
     1561 cttagattga ataaatgaat aatcactgga attgatgcag caagcaatcc gaataaaata
     1621 gcgggattaa gaaatatcat aaagtctttg tagttgttct ttttttgtga tcaaaaaata
     1681 gcaataatgc tctgagaata ctatgcgttt ttagggtaca aggaataaag ataaaggtta
     1741 tccagtttta acattaatgg atttagtaga atattcagaa attgattttg agcacttatc
     1801 ccctatttcc atataccaaa ctttttttat cctttgaaat ggaacatcaa catcttaatt
     1861 taagtgtgat aacacacaaa attaattaac ttaaagatat tatgggcgaa caattaaaaa
     1921 aatggcttaa gcaaacgcag tttgaaagtt tagatcagga agttgcatta aatatttttg
     1981 taacatctta ctttctccgt tcaaaacacg atgaagttat gggtaactac ggattaacta
     2041 tgccccaata taatgtgctc cgtatcctta aaggagttta tcctgatggt tatcctcgtt
     2101 gtgaaataat taccaggatg attgaaccgg ctcctgatgt aaccagaatt atagatagac
     2161 ttttgaagga taagttagtt gaaagattta attccaaaga agataaaaga ctttctatga
     2221 cccgcattac ggataaagga attaaattgc tggaaaaatc taatcctgaa gtgcaggcgt
     2281 taggaagtta tatttcgtca actcttaaaa attcagagaa aaaagattta tctgatttgt
     2341 tagaaaaagt ttacggatcc gtagttaagt aaccaccttt tctttgcaaa ctttgtctaa
     2401 atgttcttgg cgatctttgc ggtttctgtt tttaccacaa agtcctcaaa ggttttcaca
     2461 aagaacgcga agagtatgaa attaaaaagg cgcttcaatc ggagcgcctt aattttagaa
     2521 aactactaat cgaggctgtc ttataagagt cattccgaac ttatttcgga atctaagacc
     2581 tcgatttttc caatatttag accctgaaac aagttcaggg tgacatccaa agagtatagc
     2641 gccagcctca ttacacaaac tatttttgct tacttgcaat aagatttaat gcactgccgg
     2701 ctttaaacca ttctatttgt gcggcattaa aagtatgatt cagcatcact tcatccttgc
     2761 ttccatcttt atgttttatg atcatcttaa cttgtttttc cggagccaaa gatgaaatca
     2821 ataaatcaac tttatcatct tctcttatct tatcataatc tttcgggtcc gcaaaagtaa
     2881 gtggaagcat tccttgtttc tttaagtttg tttcgtgaat gcgtgcaaag ctttttacaa
     2941 taatagccct gcctccaaga aatcttggtt ccatcgcagc gtgttcacgt gaagaacctt
     3001 caccgtaatt ttcatcgcca accacaaccc aatttacacc tttggctttg tattcgcgcg
     3061 caacttccgg gactgatttt gtttcgccac taaaagcatt tttaactttg cctgcttcct
     3121 ttgtgtaggc atttattgca cctataaaca tattattgga aatgttatca agatgcccgc
     3181 gatactttaa ccaggggcca gccattgaga tatgatcagt tgtgcatttg ccaaatgctt
     3241 ttaataaaac tggaagatca attaaatctt ttccatccca cggagtaaat gaagtaagca
     3301 attgtaacct atcactcttc ggatcaactt taacttctac tttacttccg tcctcagctg
     3361 gagcaacaaa tccggcacta ccttcatcaa atccgcgagg tggtagctct tctcctttag
     3421 gtggatcaag tttaactttc tcgccttttt catttacaag ttcatctgta attggattaa
     3481 aagataaact tccggcaatt gcaagtgcag tagtaatttc cggtgatgct acaaatgcaa
     3541 gcgtttcagg attcccatca tttcgttttg caaaatttct gttgaatgat gttacaattg
     3601 tatttctttc accagttttt atatccatcc ttttccacat tccaatacaa gggccacaag
     3661 catttgcaag aattactccg ccaatatccg ttaaggtttt tagttgtcca tctctttcga
     3721 ttgtagctct cacctgttct gaaccaggcg tgatagtgaa ttcggattta gttttcaatc
     3781 cctttgtaat tgcttgtttt gcaatgcttg ctgagcggtc aatatcttcg tagcttgaat
     3841 ttgtacaact tccaatcaat ccaccagaaa tttgatcagt ataattttca ctctttacgg
     3901 cttccttcat tttagaaatt ggccaggctt tatcgggtgt gaacggtcca tttaaatgtg
     3961 gttcaagctc gcttaaattt atttcaatta cctgatcata atatttttcg gggttatcaa
     4021 atacctcttt atcagcacaa agttcatcag ccaaaccttc tgctaatgca gcaacatcga
     4081 cacgttttgt aattcttaag tacgatgaca ttctttcatc aaaaggaaat actgaagtgg
     4141 tagctccaat ctcagcgccc atattacaaa ttgttccttt gcctgtgcac gaaattgagt
     4201 ttgttccatc gccaaaatat tctacaattg cgcccgttcc accttttacc gttaagatac
     4261 ccgcaagctt aagtataaca tcttttgcag aagtccagcc attcatttca ccagttaact
     4321 taactccaat taactttggc cattttaatt cccatggcat tccagccata acatcaacag
     4381 catcagcacc tccaacgcct attgcgatca tcccaagtcc gccagcgttt ggagtatgtg
     4441 aatctgtacc gatcatcatt ccaccgggga aagcataatt ttccaaaaca acttgatgaa
     4501 taatacctgc acccggtttc cagaatccca cgccaaactt ctgagaaata ctttcaagaa
     4561 aatcataaac ctctttgttt tcagttgttg cacgattaag gtcttcaaca gcaccaacct
     4621 gcgcttgtat taaatgatca caatgaactg ttgatggaac tgctgtggtt tttcttccag
     4681 cagacataaa ttgaagtaaa gccatttgag ctgtagcatc ctgcatagca acacgatctg
     4741 gtgctaaatc aacataatct tttccaccgg aaaattcttt tgtcgcttta tcataaagat
     4801 gagtatataa aactttttct gcataagtta gcggacgatt aacaaccttt cgtacttcgg
     4861 taagttttgt tctgtagtta gcataaactt ttttaatcat atcataattt gccgtcataa
     4921 ttcttccttt agtaataatg atttataatt attcaaaaat aaatatttcc tactaaagat
     4981 ttctacccaa ggcaaacaat cttttaagag cactcaacat aaatacagag agattgagct
     5041 gttagatgta aattatgtcg gtaaaattta cttaaagaac caaaatatta ttaaaaatgg
     5101 tcaaaaataa ttggtgcaaa aatggaagta tccagcaagt cagttttgaa attaaggaga
     5161 atacatgtca aatggtgtac aatcgttttt gtctttgggc gcaatgatga tattcagttt
     5221 aatatcattg cgttttgata ccgctgtatt acagaataca gaagtagaag tagaaaataa
     5281 agtttactta actgcttttt ctttagccga tgatttgctg gaggaaataa agcaacgggc
     5341 ttttgatgaa aagacagttg attttaaagc tattaccacg tcagcattga ctcccagcgc
     5401 aaatttagga aaagattcag gcgaagagtg gccaaacttt aacgacattg atgattataa
     5461 tgattataca aaacctgttt cattaccgca tgcagaaaaa tatactgtaa ttagtaaaat
     5521 caactatgtt cttgaatcgg accaggatca aataagttct gatccaacat attttaaacg
     5581 agttacaatt tttgttgaaa gtccgtacct caaacatcag ataaaactat catacatttt
     5641 tacattacac tcaaagtgat attatgaatg tattaatcga tttaattggt tcatcattca
     5701 tcggaggtat tttattactt ctaattttaa aattaaatct gtatacccaa aacacaagca
     5761 tttcatcaga ttcagagtta aggatacagc agaatgcaaa gacacttgcc gaaattataa
     5821 actatgatct aagaaaagtc gggtacaact ataataacac agcaatgatt actgcagatt
     5881 ccaacaaaat taaattttat gctgatatga atgaaccggg taccgcagga tatggaacaa
     5941 tagatgttgt tgaatatttt ctaagcagcc cttcaaaagc ttcctctaca acaaatccaa
     6001 aggatgttgt tcttacacgc gtattaaatg gtacagacac tttaagcgga ccctcactag
     6061 gcctcgtcaa gctggtattt tcttattata ataagggatt agttaccaca accaatttgg
     6121 acagcataaa gtatattaaa acagaatttt gggttgagtc tttagaaccg atagaagtta
     6181 actttggaac tcaaacaggg tatccattta cttattggga aatgacaatt aatccgagga
     6241 atatatagaa tgggaaaaag tattttactt attacactgg ggatttcagt aataatttca
     6301 ttattcattt taaaactcaa tgcaaattcc aaagaaggtt taagtgtttc tgtaaatatg
     6361 tttgatcaaa atcaagcacg attaatagca aattcaggcg ttgaagtcta tcttgaaaaa
     6421 cttaaagctg ataaaagtct gattgataaa acatttacag gcaataatct atttggagga
     6481 agttatgata tctccatcga aggtcctgat accttagtta aagtaacatc ctttgcaaaa
     6541 ttccaggatg taacccataa aacaattgta tttgccaaag cagataagat acctctaatc
     6601 cctggccctg gagctatgta cttaacagcc aacacaataa gttcttttaa aaaatccgga
     6661 ataggcggca gtgttttaat tgatggcaat aatcatgata ttaatggaaa tttacttggc
     6721 gatggaattg taactccggg catagcggtc gatggcattg atcaaagaaa tgatgttatt
     6781 aatatcataa aatcaaatgc aattgaccag gtaattggtt ctggtggttc accgagtgtt
     6841 gccactataa ataataccac tgattggaat gaatacgctc ttcaattagt tgaaaatccg
     6901 gatataatta ttgatacaca agataaaatt aaaactatga ataactgggg aactgttgca
     6961 gatccaaaag taacatttgt aaatggagac atttctatca ataattctga gacagcagaa
     7021 ggatgcggca ttttggtagt gaacggaaac ctttcaatta atggaaactt tacatacaga
     7081 gggttgataa tagcatacaa agaaactact atagaaataa aacttaatgg taatggtaaa
     7141 atctatggtg gaatagtggt ggcaggacaa agtattgtta tcgaagttgc aagtggaaat
     7201 tttacaacaa aatacagtat gccggcaatg aatcatgtgg cgacactgct gaaaacaaac
     7261 agatttcaaa tagttagctg gtgggagtaa tttttacgtt tttattaaag tttctttatc
     7321 catactgtac tgttttgata aaatattgat ttttttactt tattaaattc aaaaatttca
     7381 atttagtcta tggcttgata attgaacaat agaacgttaa taactaatag aacaagagaa
     7441 attcaaaaat gagcaatata caatcaatgt tagcaatagg atctttgttt ttgctttcaa
     7501 tggtatcact taattttaat agtgccctga tgcagagtaa taccgctgaa ttagagaaca
     7561 aagtttattt aactgctttt tcattagcag atgatctaat tgaagagatt aagcagaagg
     7621 gttatgacga gaaaactctt gattttccaa cagtgaatcg ttcaaccttg actcctgctt
     7681 tagcattagg cccagaaagt gaagtttatc ctttttttga tgatatagat gactataata
     7741 attactcaag agatgcagat gccccacact cagaaaaata tcatatatct gctaaagtct
     7801 attatgtatc agaaactaat ccggacacta aaagtgttac acaaactttt cataaaagag
     7861 tggatgtaac tgtaagcagc ccttatttaa ataaccctgt agtattaact tttatattta
     7921 cacataaata atatggcatc tttaatagat atacttggtt ccagttttat tggtggttta
     7981 ttgctgctgc tattacttaa actgaattta tttgcaaaca gcagtagcgt tacttctgat
     8041 aatgagctta agcttctgca gaatgcaaaa actttagcgg aaattattaa ttatgatttt
     8101 agaaaaattg gttatgagta tgacggaaca gctatcgcga ttgcagactc aaaccacatt
     8161 aagttttatg ctgatataga tagaaataat acgatagata ctcttgagta ttatacaagc
     8221 gatacgactg tagctaccgg cacagaaaat ccaagagata taattttgtt tagaaaatat
     8281 aatggcgtcg caatgggtgg tccaacattg ggattaacaa aaattaaatt gtcttactta
     8341 aatgagttta atgttgtcac caatgtttta gccaatataa agtacattaa agttgagttg
     8401 tgggttgagt caacaattcc agaaatggag tctaactttg ttaacccaaa tcacccctat
     8461 ctttttactt attgggaaat gaaaattaat ccaaggaata tataattatg ggaaagatgt
     8521 cattaatatt aacaatgggt attgccgtaa taatcggttt ctttgtgttg cgactgaact
     8581 caaacgcaaa gcaaggatta agtacaacac ttgatatgta tgaatataca caggcaaggc
     8641 tcatcgcgaa ttccggtgtt gaaatttatc ttgaaaaaat gcgaagagat aaaaccatta
     8701 atagtggaac ttatttaaat aacgatctgg cagatggaag ttatgatata tatataactg
     8761 gacctgatac tgctctcaat attagatcag tagctcattt tatgaatgtt acgcacgaaa
     8821 caattgttaa agccaaaaga gaccctataa aatttcctac agcacccgga gccttatatg
     8881 tttcaacctc agctatgtct aatgttatga tgaccggtaa ttttactgta agcgggttta
     8941 accacgataa aaatggaact ttagtaagta ccactaataa tacagtccct ggtgtagtag
     9001 tagataataa tgctgatagt attgcaatcg taagcatttt gaaaaaaaac actgcaaata
     9061 atattacagg caaaggtggt ggaacgccaa atgtcgctgt tatgccacaa ggctcagatt
     9121 gggccgccat ttcaaatgat attgcgtttt cagctgataa aacaatagga agcggaatat
     9181 ataatactgg tgaattcgga acttgttcaa atcctcaaat tactttggta aacggcgatg
     9241 tagaatttaa tggtagtttg acaggtgcgg gaatattagt agttaatggt gatttgacaa
     9301 ttactggaac ttttaagtgg aaaggactaa ttattgctta taaagaatcg gcaataacaa
     9361 caaaattaaa tggaactgga accgtgattg gatctttaat agtttcgggt aattcagtag
     9421 atatgaatat ctctaatgga acttttgaag cactctacag cccagaagct ctaaataatg
     9481 ccaaattaaa tctaaaatct tcacgattta aaatacttag ctggtgggaa taaattttag
     9541 tgttattttc tagccgcctt gtaattgaag taaaacaata atctttgctt taattctggc
     9601 ggcatttttt aaaagttaat tgaagtaata aagttctccc atattcttgt aaataattcc
     9661 tatctgcttg cctaaaaagt tccacaaccc aaaaattact taaaaaattc attcttcaga
     9721 taaaaattaa atagcatgat aattgatatt ttctgccaaa tattactcca aaattgacgg
     9781 ggtaatatat ttgaatatcg gtcctgtaaa aaattattgt taactggaat attcatttca
     9841 atatttttac atttagaaaa acaaacaggt ttttagtttg aaagaaacaa tcttattagt
     9901 agatgatgaa ttaacctatc tggaactgct aaagagcata cttcagcagg aaggttacag
     9961 caatgtaatt acagaatcca atcctttaa
//
LOCUS       DASWBY010000614         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_28474, whole genome shotgun sequence.
ACCESSION   DASWBY010000614 DASWBY010000000
VERSION     DASWBY010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28474"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VF870_11195"
     CDS             <1..1416
                     /locus_tag="VF870_11195"
                     /inference="COORDINATES: protein motif:HMM:NF036809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEX9252799.1"
                     /translation="SNMNMLRVWGGGIYEHDIFYDLCDEKGILVWQDFMFANSMYPGD
                     SLMFQNIALETTEQIKRLRNHPSIALFCGNNEIAEAWANWGWKNKYSRDQQKKMDNDY
                     NQIFHKLLPDIVNKFSPQITYWPSSPQFGRGDKRSQYEGDSHYWGVWHDEEPFEMYEQ
                     KVPRFMSEFGFQSYPSLKTLENWIEKKDLNFNSPMLQSHQKHPRGNELIKKYMEWNYK
                     VPYSFSDFIFVSQLLQTEGIAMGIEAHRRAMPYCMGSLYWQLNDCWPVVSWSGIDYEE
                     RWKALQYVVKKSFEPVILSFERKDELLNIFLVNDTNESVNGYLTLRLMNFDGEKKLQA
                     EEYYQINPNSVKLLRSIDLTNYNRFKWENSFLNIRFNGDVNAAEKNFYFVKPKDMSLS
                     KPEIKISKEKNGDHYILNLVSNTLVKNVFVDCNADGKFSDNFFDLIPREEKIIEFYPN
                     EDIREVSFTTFSLWDTMQNNN"
     gene            complement(1413..1967)
                     /locus_tag="VF870_11200"
     CDS             complement(1413..1967)
                     /locus_tag="VF870_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9252800.1"
                     /translation="MNIHFSPFPILQTERLILRKLSLDDSEEIFFLRSNDEVNKYLDR
                     TRAVDLDDALGFINKVNIGIANDKWMYWAVCFKDNPKLIGTVCLWNLNKKENKAEVGY
                     ELMPEYQRKGIAQESLYAVIGFGFSKLELGKIEAYTNKENLASIKLLKKLSFVRDHNE
                     EIKIDFSIENPKTTVYSLLKTNNN"
     gene            complement(1973..2314)
                     /locus_tag="VF870_11205"
     CDS             complement(1973..2314)
                     /locus_tag="VF870_11205"
                     /inference="COORDINATES: protein motif:HMM:NF038084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HEX9252801.1"
                     /translation="MSTIPFQVIDWNKSRKEEHKGETGTSFWQSLQYDGLRVRIVEYS
                     DGYLADHWCKKGHIVHCLEGEVLNEQENGEKHILKAGMTYIVSDNLSSHRSVAKEKVK
                     LLIIDGDFLKQ"
     gene            complement(2318..2905)
                     /locus_tag="VF870_11210"
     CDS             complement(2318..2905)
                     /locus_tag="VF870_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX9252802.1"
                     /translation="MQKELCAFFPTQKHLRIWFEKNYKKEKELFVGFYKGSSCKPTVN
                     WSQSVDEAICFGWIDGIRKSIDDESYCIRFTPRNPKSNWSAVNIKKAESLTKLGLMKP
                     EGLKAFSYRKEEKSKIYSYENQVVGFDKSYEKRFKANKQAWKYFQSTAPSYQKITTRW
                     VMSAKQETTKLKRLNELINDCALGKKIKAMNYRKK"
BASE COUNT          964 a          499 c          503 g         1024 t
ORIGIN      
        1 tcgaatatga atatgcttag agtatggggc ggtggaattt atgagcacga tattttttat
       61 gatctatgtg atgaaaaagg aattcttgtc tggcaggatt ttatgtttgc aaatagtatg
      121 tatcccggtg attcattaat gtttcagaac atagctttag aaacaaccga gcaaatcaaa
      181 agactacgca atcatccatc gatcgctcta ttctgcggaa acaacgagat agcagaagcc
      241 tgggcaaact ggggctggaa aaacaagtat tcaagggatc agcagaagaa aatggataat
      301 gattacaatc agatatttca taaactttta ccggatattg ttaataagtt ttctcctcaa
      361 attacatatt ggccatcatc tccccaattt ggaagaggtg ataagcgcag ccagtatgaa
      421 ggggactcac attattgggg cgtgtggcac gatgaagagc cttttgaaat gtatgagcaa
      481 aaggttccaa gatttatgag tgagtttggg tttcaatcat atccatcatt aaaaacttta
      541 gagaattgga ttgagaaaaa agatttaaat tttaactcac caatgcttca atcacatcag
      601 aaacatccga ggggaaatga acttattaaa aaatatatgg agtggaatta taaagttccg
      661 tatagttttt ctgatttcat ttttgtaagc cagctattgc agactgaagg tatcgcaatg
      721 ggaatagaag ctcatagaag agcaatgccc tattgtatgg gatcgctcta ttggcagtta
      781 aacgattgct ggcctgttgt ttcgtggtca ggtatcgatt atgaagaaag atggaaagcg
      841 ctgcaatacg ttgttaaaaa aagttttgag ccagtaattt tgtcttttga aagaaaagat
      901 gagttactaa atatttttct ggtgaacgat acaaacgaat cggttaatgg ctacctgact
      961 ttgcgattga tgaattttga tggtgaaaag aaactacaag cagaagaata ttatcagatc
     1021 aatcctaatt ctgtgaaatt gctaagaagc attgatctaa caaattataa tcgttttaaa
     1081 tgggaaaatt catttttgaa tataagattt aatggagatg taaatgcagc cgagaagaat
     1141 ttctattttg taaaacctaa agatatgagc ctatcaaaac ctgaaattaa aataagtaaa
     1201 gaaaagaatg gcgatcatta cattctcaac ttagttagca atactttggt aaaaaatgtt
     1261 tttgttgatt gtaatgcaga cggaaaattt tcagacaatt tttttgatct gatcccgaga
     1321 gaagaaaaga taattgagtt ttatcctaac gaagatattc gtgaagtttc gtttacaact
     1381 ttttctttgt gggatacaat gcaaaacaac aattaattat tgttcgtttt caacagactg
     1441 taaacagttg ttttcgggtt ttcaatagaa aaatcgatct taatttcttc attatgatct
     1501 cttacaaaac ttaacttttt tagtaatttt atcgaagcaa gattctcttt gtttgtatac
     1561 gcttcaattt tcccaagttc tagtttgcta aatccaaacc cgatcactgc atacaaagat
     1621 tcttgtgcaa ttcctttgcg ctgatattcc ggcatcaatt cataaccgac ttcagctttg
     1681 ttttcttttt tatttaaatt ccataaacaa acagtaccta ttagttttgg attatcttta
     1741 aaacaaacag cccaatacat ccacttgtca tttgcaatgc cgatattaac tttgtttata
     1801 aatcctaatg catcatcaag atcaacagct cttgttctat caagatactt gttcacttca
     1861 tcattagaac gaagaaaaaa tatttcttct gaatcatcta atgaaagctt tcttaagatc
     1921 aacctttcag tttgtaatat tggaaaaggg ctaaaatgta tattcataga ttttattgtt
     1981 taagaaaatc accatcaata attagaagct taactttttc ttttgcgacc gaacgatgcg
     2041 aacttaagtt gtcagaaacg atataagtca tgcctgcttt taagatatgc ttttcaccat
     2101 tttcctgttc atttaaaact tcaccttcaa ggcaatgcac aatgtgtcct tttttacacc
     2161 aatgatcagc aagatagcca tcgctgtact caactatgcg tacacgcaaa ccatcgtatt
     2221 gtagcgattg ccagaatgag gttccggttt ctcctttgtg ttcttctttc cgagatttat
     2281 tccaatcaat tacctgaaaa gggatggtgc tcataaatta tttctttcgg taattcatag
     2341 cttttatttt ttttcctaaa gcacaatcat taattagttc gtttaatcgt tttaattttg
     2401 ttgtttcctg ctttgcgctc ataacccagc gagttgtaat tttttgatat gatggagccg
     2461 ttgattgaaa atacttccat gcctgcttat ttgctttaaa ccttttttca taagatttat
     2521 caaaacccac cacttgattt tcataagaat agatttttga tttttcttct ttgcgatatg
     2581 aaaaagcctt taacccttct ggtttcatta agccaagctt tgtaagactc tctgcctttt
     2641 taatatttac agcactccaa ttgcttttgg gatttcttgg agtgaaacga atgcagtaac
     2701 tttcatcatc aatacttttt cgaataccat caatccagcc aaaacaaatc gcttcatcta
     2761 cagattgaga ccagttaaca gtgggtttgc agctagatcc tttataaaag cccacaaaaa
     2821 gttctttttc ctttttgtaa tttttttcaa accaaattct taaatgtttt tgcgttggaa
     2881 agaaagcaca gagttctttt tgcattaaga ttttggtttg ttaaagaatt tataaaaaat
     2941 aaaaggagca attgttatcc atactaaaaa taaaccccaa acccttcctt
//
LOCUS       DASWBY010000615         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_621330, whole genome shotgun sequence.
ACCESSION   DASWBY010000615 DASWBY010000000
VERSION     DASWBY010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621330"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VF870_11215"
     CDS             <1..230
                     /locus_tag="VF870_11215"
                     /inference="COORDINATES: protein motif:HMM:NF036482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEX9252803.1"
                     /translation="NIKVTNDKGSTTLLIKSKNPVFFIDIYYPKLTFSDRGFFLLPGE
                     QIKIKVEESKLESFKESDFKIYTLNDYLYLK"
     gene            253..1221
                     /locus_tag="VF870_11220"
     CDS             253..1221
                     /locus_tag="VF870_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9252804.1"
                     /translation="MQYSKLGRSGLLVSRLCLGTMNFGAITPEKDSYEIMNRALELGI
                     NFFDTANVYGGKMGAGITEKIIGKWLEENKERRDKIVLATKVYGEMGKWVNERYLSAY
                     HIKKACEDSLKRMKTDHIDLYQMHHVHRDTPWDEIWQAMEQLISEGKIIYVGSSNFAG
                     WDIATANEEARRRNLLGLISEQSKYNLFNRFIEREMVPACNYYGVGIIPWSPLEGGLL
                     GGVLKKLDKGRRATDEIVNKLKNNHQKIAQWESLCDEIGEDPANTAIAWLLNQPGVTA
                     PIIGPRTIKQFEDSIRSLDINFNQELLSKLDEIFPPAGTSPQYYAW"
     gene            1251..>1712
                     /locus_tag="VF870_11225"
     CDS             1251..>1712
                     /locus_tag="VF870_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252805.1"
                     /translation="MFKYCLYKVQINIILLFLFSFIKIDAQQINISRIEQMPNLPAPF
                     EMRDWKKVTAGYDSLVFDLNRTGQYLPLIWLNNNTINYPEHNSFGLHTVVGTTSPFSS
                     ESINLLPATIGASLTGIDKSNQNGYNWVLMCEEYFNKSNNAMVYLNHPNGSN"
BASE COUNT          612 a          257 c          340 g          503 t
ORIGIN      
        1 taaatattaa agtaaccaat gacaagggtt ctactactct attaataaag tctaagaacc
       61 cagtcttctt tattgatata tattatccaa aactgacttt ttctgacaga ggctttttct
      121 tattgccggg tgaacaaatt aaaataaagg ttgaagaaag taaattagaa tcttttaaag
      181 agtctgattt taaaatttac actttaaatg attatttata cttaaaataa attatacatt
      241 gaaagaataa caatgcaata ttcaaaatta ggaagaagcg gattgctagt cagcagactt
      301 tgtctcggaa caatgaattt tggagcaatc acaccagaaa aagatagtta tgaaatcatg
      361 aatcgtgcat tagaacttgg tatcaatttt tttgataccg caaatgttta tggcggtaaa
      421 atgggagcgg gaataactga aaaaattatc ggcaaatggc tggaggaaaa taaagaaaga
      481 agagataaaa ttgtactggc aacaaaagtc tatggagaga tgggtaaatg ggttaatgaa
      541 agatatcttt cagcttacca tatcaaaaaa gcctgtgaag atagcctaaa aagaatgaag
      601 actgatcaca ttgatttgta tcagatgcat catgtccatc gtgatacacc gtgggatgaa
      661 atatggcagg caatggaaca attaatttca gaaggtaaaa ttatctatgt aggaagcagc
      721 aactttgcgg gttgggatat agcaacggct aatgaagaag ctcgaagaag aaatttactt
      781 ggtttaatta gtgaacaaag taaatacaat cttttcaacc gatttataga gcgtgaaatg
      841 gttccggcct gtaattatta tggagtagga ataattccct ggagtcctct ggaaggcgga
      901 ttacttgggg gagtattaaa aaaattagat aaaggccgca gagcaaccga tgaaattgta
      961 aataaactga aaaacaatca tcagaaaata gcacaatggg aaagcttgtg tgatgagata
     1021 ggtgaagatc cggctaacac cgcaattgca tggttattaa atcagcccgg ggttacagcc
     1081 ccgataattg gaccccgtac tattaaacaa tttgaggact cgattcgttc tttagatatt
     1141 aattttaacc aggaattact ttcaaaattg gacgagatat ttccaccggc cggaacttca
     1201 ccgcagtatt atgcctggta aaatgtgttt cccaagattt aggagtaaat atgtttaaat
     1261 attgtttata taaagttcag ataaacatca ttttattatt tttattctca tttattaaaa
     1321 ttgatgcgca gcaaataaat attagccgca ttgaacaaat gcctaatcta cccgcacctt
     1381 ttgaaatgag agactggaaa aaggttactg ccggatatga tagtctggtt tttgatttga
     1441 atcgcaccgg gcaatatctg ccattgatat ggttgaacaa taacacaatt aattatccgg
     1501 aacataacag ctttgggctt cacactgttg ttgggactac ttctccattt tcttcagaga
     1561 gtataaacct tctgccggca actatcggag cttcactgac aggcatagat aagagcaatc
     1621 agaatggata taattgggta ctaatgtgcg aggagtattt taataaatca aataatgcta
     1681 tggtttatct taatcatcca aatggaagta at
//
LOCUS       DASWBY010000616         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_360997, whole genome shotgun sequence.
ACCESSION   DASWBY010000616 DASWBY010000000
VERSION     DASWBY010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360997"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="VF870_11230"
     CDS             complement(<1..135)
                     /locus_tag="VF870_11230"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX9252806.1"
                     /translation="MYKKLYRSVTDKMVGGVCGGLAEYFAIDPVIVRLIFVLAVIFGG
                     S"
     gene            366..2003
                     /locus_tag="VF870_11235"
     CDS             366..2003
                     /locus_tag="VF870_11235"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX9252807.1"
                     /translation="MKIALCQMDPIIGHLEYNKQKILDGYKKAIESKADLAIFPELSL
                     VGYPPLDLVEKQEFRKAVNEASLEIASITKEVGLIFGAITEDNDNVGTDIHNSAILCF
                     DGKIQFVQHKTLIPNYDVFDEMRYFDSAESVSVFEFKGEKLGISICEDIWNDADYWYR
                     RRYRKDPVDDLLKQGITILINISASPYAFGKRCSRQDMLSTLTKKDKIPLAYVCCVGA
                     QTDLIFDGASMCFDDNGNLVKLGKAFNEDFILFDTKIKYDPIKICEGTFEEEVYNSLV
                     FGLREYCSKLGFKKVVVGLSGGMDSALVTCIAVEALGADNVNVILLPSKYSSEGSITD
                     SELLISNLGIQSGNVSIQPVVDETITQIKPMLKNDIKSLTEENLQARVRGLYLMAYSN
                     NFGNLLLTTGNKSEMAVGYCTLYGDMCGGLAVIADLYKTDVYRVAKYINRKKQIIPQV
                     IIDKAPSAELKHNQTDQDTLPPYALLDKILRMYLEENKEYDEIKEEIGDYELVKRVLR
                     MVDMNEFKRYQAAPALRVSSKAFGYGRRYPIVQGWRK"
     gene            2119..>2923
                     /locus_tag="VF870_11240"
     CDS             2119..>2923
                     /locus_tag="VF870_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX9252808.1"
                     /translation="MAKEKEVQQLSEVTVRFAGDSGDGMQLTGSQFSDTTAFVGNDLN
                     TLPDYPAEIRAPAGTIYGVSGFQLHFSSEDIHTPGDQPYVLVAMNPAALKKNLAEIKK
                     NGIIIVNTDAFDLKNLKLAHYESNPLEDDTLEGYTVFGVPISKLTENALEGTPLSPKE
                     VARCKNFFALGLMYWLYNRPIQNTIDWIQDKFKKHPQFIDANTKALNAGFNYGEMTEL
                     FTTRYDVISARLPKGTYRNISGNEATAFGFLAASVQSGLPLYLGSYPITP"
BASE COUNT         1002 a          443 c          571 g          907 t
ORIGIN      
        1 actgccacca aagattactg ccaaaacaaa aatcaatctt acaatgacag gatcaatcgc
       61 aaaatattct gctaatcctc cgcaaacccc accaaccatt ttgtctgtta cggaacgata
      121 aagtttcttg tacatctcag aactcctttc tgtatctaat ttctaatgca aaactaatca
      181 agaaatacac agaaggcttt ttttatggta tcaacggttg atgattatta aagttgggta
      241 aatcagttta ttgtcgggcc aatggtatcg gtgaacggca ggaaaatttg attatttatt
      301 gtattaatta aaacttagtt tattttagtt gttagattta aataaaaaat gaagtaaatc
      361 attccatgaa aatagctctt tgccagatgg atccaatcat tggtcatctg gaatataaca
      421 agcaaaagat tcttgatgga tataagaaag ctattgaatc gaaagccgat cttgcaattt
      481 ttcctgaact ttcattggtt ggttatccgc cattagatct tgttgaaaaa caagaatttc
      541 gcaaagctgt taatgaagct tcactagaaa ttgcatcgat aacaaaagaa gtagggttaa
      601 tttttggggc aataactgaa gacaatgata atgttggaac tgatattcat aattcagcta
      661 tattatgctt tgatggaaag attcaatttg ttcagcataa aacattaatc ccgaattatg
      721 atgtatttga tgagatgcga tattttgatt ctgcggaaag cgtttcagta tttgaattta
      781 aaggtgaaaa gttagggata tcgatttgtg aagacatctg gaatgatgct gattattggt
      841 acagaagaag atatcgtaag gatccggttg atgatttact aaaacaggga attacaattc
      901 tgattaatat ttcagcaagc ccatatgctt ttggaaaaag atgctcacgt caggatatgt
      961 tatcaacact cacaaaaaaa gataaaattc cacttgccta tgtttgctgt gttggagcac
     1021 aaacagattt gatttttgat ggcgcaagta tgtgttttga tgataatggg aatctcgtaa
     1081 aacttggtaa agcttttaac gaagatttta ttttatttga tacaaaaata aaatatgatc
     1141 cgataaaaat ttgtgaaggt acttttgaag aagaagttta taactcgctt gtatttggat
     1201 tgagagagta ttgcagcaaa ttaggattca aaaaagtagt agttggatta agtggtggaa
     1261 tggattctgc tttagttaca tgcatcgcgg ttgaagcttt aggtgcagat aatgtcaatg
     1321 taattctctt accgtcaaaa tattcaagtg agggaagtat tactgattct gaattgctga
     1381 tttcaaatct aggaattcaa tcgggtaacg taagcataca acccgttgtt gatgaaacaa
     1441 ttactcaaat taaaccgatg ctcaaaaatg atattaaatc actgacagaa gaaaatttac
     1501 aagcaagagt tcgaggttta tacctgatgg catattcgaa taattttgga aatttgttat
     1561 taacaaccgg taataaatct gaaatggctg taggatattg cactttgtat ggcgatatgt
     1621 gcggcggttt agcagttatt gctgatcttt acaaaacaga tgtttataga gtagccaaat
     1681 atattaatag aaaaaaacaa ataattcctc aagtaattat cgataaagcc ccttctgcag
     1741 aacttaagca taatcaaact gatcaggata ctttaccgcc ttacgcactg ctggataaaa
     1801 ttttaaggat gtaccttgag gaaaataaag agtacgatga gataaaagaa gagattggtg
     1861 attacgaatt ggtaaaacga gtgttaagaa tggttgatat gaatgagttt aagcgatatc
     1921 aggctgctcc cgcattgcgt gtttcatcca aagcatttgg atatggaaga agatatccga
     1981 ttgttcaggg atggagaaaa taatcctaga aagaaattca gcttaatacg ccttaaggtt
     2041 attttgaaat caattgtaaa ttcataacag tcttattagt tttgaaatag caaatgttta
     2101 atattataag tgagaaaaat ggctaaagaa aaagaagttc aacagcttag cgaggttacc
     2161 gttcgctttg caggcgattc cggagatgga atgcagttga ctggatcgca gtttagtgat
     2221 actactgctt ttgttggtaa tgatttaaat acacttcctg attatcctgc agaaattcga
     2281 gctcctgccg gaacgattta cggtgtaagt ggattccaat tacattttag tagtgaagat
     2341 attcacacac caggtgacca gccttatgtt ttggttgcaa tgaatcccgc agcattaaaa
     2401 aagaatcttg ctgaaataaa aaagaatgga ataataatag taaatacaga tgcttttgat
     2461 ctcaagaatc tgaagttagc acactatgag tcaaatccat tggaagatga cacactcgaa
     2521 ggttacactg tttttggtgt tccaataagt aaacttaccg aaaatgcact tgaaggcaca
     2581 ccgctttcac caaaagaagt tgcaagatgt aaaaactttt ttgctcttgg tttgatgtac
     2641 tggctttaca atagacccat tcaaaatacc attgattgga ttcaggataa atttaagaaa
     2701 catcctcagt ttattgatgc taacacaaaa gcactgaatg ccggatttaa ttatggtgag
     2761 atgaccgaat tgttcactac aagatacgat gttatctctg ccagactgcc aaaaggcaca
     2821 tacagaaata tttccggaaa cgaagcaact gctttcggat ttcttgccgc ttcagttcaa
     2881 agcggattac ctttgtattt gggttcatat ccaattacac cgg
//
LOCUS       DASWBY010000617         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_703904, whole genome shotgun sequence.
ACCESSION   DASWBY010000617 DASWBY010000000
VERSION     DASWBY010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703904"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VF870_11245"
     CDS             complement(<1..471)
                     /locus_tag="VF870_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEX9252809.1"
                     /translation="MRGFEHPDVFEFRHIGPSTDEINEMIKICGVNSVDELIDETVPS
                     NIRLKGKLNLDEPLSEFEFINYLKSIAKKNKVYKSYIGMGYYPSILPSVIQRNVLENP
                     GWYTQYTPYQAEIAQGRLEALLNFQTAITDLTALPIANASLLDEATSAAEAMIMF"
     gene            complement(609..1382)
                     /gene="rnc"
                     /locus_tag="VF870_11250"
     CDS             complement(609..1382)
                     /gene="rnc"
                     /locus_tag="VF870_11250"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX9252810.1"
                     /translation="MSSLFSRLLKYFGFRDESNNSHQVKKILTPLKFSELEEIIGFPI
                     KNKSHYIQALMHRSFLEELEEDDTSNERLEFLGDSVLSLVVAEYLFENFPKENEGFLT
                     KVRAKLVNRFALSDAAEDIGLAKFILINQNLSNTFARASKTVLSDALEAVIGAIYLDH
                     GLEISKKFIHRILIEPLTKDGEYLIDENYKSQLLEYAQANKIEVPNYIVIKEEGPQHD
                     RVFTVRVNVGLDYFGIGKGKNKKTAEQNAAQAALEKILH"
     gene            complement(1385..>1897)
                     /locus_tag="VF870_11255"
     CDS             complement(1385..>1897)
                     /locus_tag="VF870_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX9252811.1"
                     /translation="NHALNRGAKIYAEIAGIGLTADAHHITAPAPGGEGAVRSMKIAI
                     EDAGLLLTDIGYINAHGTSTPHNDVNETRAIKTLFGDHAYKLIVSSTKSMTGHLLGAA
                     GGIEAIATLLSMKNSLIPPTINLDNPDPECDLNYSAKKVTEKKINAAICNTFGFGGHN
                     ASILFKKFEE"
BASE COUNT          562 a          348 c          303 g          684 t
ORIGIN      
        1 gaacataatc atagcttcag cagcggatgt tgcttcatca agcaaagatg catttgctat
       61 tggtagtgcg gtaagatctg ttatggctgt ttgaaaatta agcaatgctt ccaatcttcc
      121 ctgagcaatt tctgcttgat atggtgtgta ctgtgtgtac caacccgggt tttctaaaac
      181 atttctttgg attactgaag gaagaattga aggatagtat cccattccaa tataactttt
      241 ataaacttta ttttttttag caatactttt taagtagtta ataaattcaa attcacttaa
      301 aggttcatcc agattcagtt tacccttaag gcggatgttg gaaggtacag tttcatcaat
      361 tagttcatca acagaattta ctccacatat tttaatcatt tcgttaattt catcagtact
      421 tggccctatg tggcggaatt caaatacatc aggatgttca aagcctctca ttttaattct
      481 cttaatttat tcaatattaa aaaactacat taattttatt gttcaaaatt acggaaaatc
      541 tatataattc tgattgagtt aactggttat tttaggacaa ttttttgttg attactggtc
      601 acaaagacct aatgcaggat tttttccaga gcggcttgtg cagcattttg ttctgccgtt
      661 ttcttgttct tcccttttcc tatgccaaaa taatctagtc caacatttac tctaactgta
      721 aaaaccctat cgtgctgcgg tccctcttct tttattacaa tataatttgg aacttctatt
      781 ttgtttgcct gagcatattc taaaagctgg cttttataat tttcatctat caaatattcg
      841 ccatcttttg ttaagggttc aattaaaatt ctatgaatga atttcttaga aatttctaat
      901 ccgtggtcta aataaattgc accaataact gcttcaagcg catctgataa tacagtttta
      961 gaagcacggg caaatgtgtt ggataaattt tgattgatca aaataaattt tgcaagtcca
     1021 atatcttcag cagcatctga taatgcaaat ctgtttacaa gtttggctct aactttcgtt
     1081 aaaaatcctt cattctcttt gggaaagttt tcaaaaagat attcagctac aacaagactt
     1141 aatacagaat cacccaaaaa ttctaatctc tcatttgaag tatcatcctc ttcaagttct
     1201 tcaagaaatg atctatgcat taaggcttga atgtaatggg atttgttctt gattggaaaa
     1261 cctattatct cctctaactc agaaaacttt agtggtgtaa gtattttttt aacctggtgg
     1321 gaattattgc tttcatcccg aaagccaaaa tatttaagca aacgagaaaa aagcgagctc
     1381 aaaactattc ctcaaacttc ttaaataaaa ttgatgcatt gtgtccgcca aatccaaagg
     1441 tattacaaat tgcggcatta attttctttt cagtaacttt ttttgcacta taatttaaat
     1501 cacattcagg atcaggattg tcaagattta ttgtcggcgg tattaaacta tttttcatcg
     1561 aaagcagtgt ggcaattgct tcaattccgc ctgcagcacc taacagatgc cctgtcatag
     1621 actttgttga gctaacaatt aatttgtatg catgatcccc aaacaatgtt tttattgctc
     1681 ttgtctcatt cacatcatta tgtggtgtgg atgttccgtg tgcatttatg taaccaatat
     1741 cggttagtaa gagcccggca tcttctatgg ctattttcat tgatctgaca gctccttcgc
     1801 caccaggagc aggagcagtt atatgatgtg catctgcagt aagcccgata ccggctattt
     1861 ctgcataaat ttttgcgcct ctatttaatg catgatt
//
LOCUS       DASWBY010000618         4063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_59775, whole genome shotgun sequence.
ACCESSION   DASWBY010000618 DASWBY010000000
VERSION     DASWBY010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59775"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VF870_11260"
     CDS             complement(<1..769)
                     /locus_tag="VF870_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252812.1"
                     /translation="MQEIKIKKVETKSDLNTFIKCQWKFYKNDSYWVPPLLMERKTLL
                     SKEKNPFFKHAEADHFIAYRNNEIVGRISAIKNDMYLKYHNDNSGFFGFFECINDQEV
                     ANALFDTAKKWLKEKGLSRMLGPANPSSNDEYGMLLEGFEDSPRLLMTYNPKYYLDLC
                     SNYGMTKAKDLLAYKLENHKVMGSEKLKRVQQIARDRYKLKISQLDMKNFAKELEKVK
                     FVYNKAWAPNWGFIPMTDEQIDAMAKDLKPLVEPSLIL"
     gene            complement(833..2023)
                     /locus_tag="VF870_11265"
     CDS             complement(833..2023)
                     /locus_tag="VF870_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEX9252813.1"
                     /translation="MDIFKKCQEFTRADEIKAMGVYPYFRPIEANEGPVVQIEGKRII
                     MAGSNNYLGLTGHPQVKEAAIKAIEKYGTGCSGSRYLTGTLDLHIELERRLAKFFNTE
                     AVLLFSTGYQSAQGVIPTLVQRNDFVISDKDNHACIVAGTLMTKGAMGGLVRYKHNDM
                     DDLEKTISKLPAETGKLIVSDGVFSTGGEIVELPRLIEIAKKYGARVMIDDAHSVGVI
                     GKGGRGTASEFNLEKDVDLTMGTFSKTFASLGGFVAGTERVINFIKHFSPALIFSASP
                     TPASVASALAALDVLEAHPELVTRLIDNANYMRTNLKAKGFNIIDGRTAIVPVIVGDD
                     AIAFKMWKLLYDNGVFVNVFISPGVPPGRQMMRTSYMATHEKEHLDEILHIFEKAGKA
                     VGLI"
     gene            2130..3122
                     /locus_tag="VF870_11270"
     CDS             2130..3122
                     /locus_tag="VF870_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9252814.1"
                     /translation="MQTQEIALVTGANGFVGSHLVDNLLTKGLKVRCLVRKSSKLKWL
                     DKKDVEIFDTGLFDKDGLRKAFKDVNYIFHVAGVVKSKTKEGYFKGNVETTRNLLEVA
                     LENSSIIKRFLVVSSQTVSGPSTEGNPVNENTECNPITTYGKSKLEEEKLVLSYKDKL
                     PITICRAPAVYGERDTEIFIYFQTFSKGLTTTIGFNKKELSLIHAVDLAEGFYLAAMS
                     EKAKGEIYFIGSEKYYTWQEINSITSKTLNKKPIIIKVPHFLVYTIAAVAQFFAMFSS
                     KPATLNIEKAKDITQQYWTCDTSKAIRELGYRQNVSIEAGIKRTCDWYKQMKWI"
     gene            3208..>4063
                     /locus_tag="VF870_11275"
     CDS             3208..>4063
                     /locus_tag="VF870_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9252815.1"
                     /translation="MQKSKVLVIDDEIDLRDLIKRLLELEGYTVFTAENAEQGLNVIK
                     SNEIDIVLSDVRLPDKNGLELIPLIKKIHPSIEIIILTAYGKIEDGVRAIKDGAFDYI
                     VKGDEDNKLLLVIKNANDKIQMFNRISHLEKNIFSKYTFDNITSKSPLIQEAISVAKK
                     AANSASPILLVGETGTGKEVFAQSIHYESERRKEPFVAINCSGFAKDLLESEMFGYKA
                     GAFTGAVKNKKGLFEEADRGTLFLDEIGDMDISLQAKLLRVLENNTFIKQGDTKESSV
                     DVRIIAATN"
BASE COUNT         1369 a          700 c          704 g         1290 t
ORIGIN      
        1 acaaaataag tgaaggctca acaagaggct ttaaatcctt tgccatggcg tctatttgtt
       61 cgtcagtcat aggaatgaat ccccaattgg gtgcccaggc tttgttataa acaaatttga
      121 ctttctctaa ttccttagca aagtttttca tatcaagctg cgatatttta agcttgtaac
      181 gatcacgagc aatctgctga actcgcttta acttctctga acccattact ttatgatttt
      241 caagtttata agctaaaaga tcttttgcct ttgtcattcc ataatttgaa caaagatcta
      301 aatagtattt aggattgtaa gtcatcaaga gtcttggcga atcttcaaaa ccttcaagca
      361 gcattccata ttcatcatta cttgatggat tagccggacc aagcattctg ctaagacctt
      421 tttccttcaa ccattttttt gcggtatcaa ataaagcatt tgctacttct tgatcgttga
      481 tgcattcaaa gaatccgaag aatcctgagt tatcattgtg atattttaag tacatatcat
      541 ttttgattgc agatattctt ccaactattt cattatttct ataagcaata aaatggtcag
      601 cttcagcatg tttaaaaaac ggattttttt ctttactaag aagagttttt ctttccataa
      661 gaagtggagg aacccagtac gagtcattct tataaaattt ccactggcat tttataaatg
      721 tgttaagatc agacttggtc tcaacttttt tgatttttat ttcttgcata tttaatccta
      781 aataaaaaaa gcatactcaa ttgagtatgc tttaaaataa attagttcga tactaaataa
      841 gtcctacagc tttacctgct ttttcaaaaa tgtgtagaat ctcatcaaga tgttcttttt
      901 catgagtagc catataactt gttctcatca tttgtcttcc gggaggaaca ccaggggaaa
      961 tgaatacatt tacaaaaaca ccattatcat aaagtaactt ccacatctta aaagctatag
     1021 catcatcacc cactataacc ggaactattg ctgttctgcc atcaattata ttaaatcctt
     1081 ttgctttaag attggttctc atataattgg cattgtcaat cagtcttgta accagttcag
     1141 gatgagcttc aagaacatca agtgcagcaa gcgctgaagc aactgaagca ggtgttggag
     1201 atgcactgaa gattaatgca ggtgaaaaat gtttgatgaa atttatcact cgttctgttc
     1261 ctgcaacgaa tccacctaag gatgcaaaag ttttactaaa agtacccata gttaaatcaa
     1321 catctttctc aagattgaat tcactagctg tacctcttcc gcctttacct ataacaccca
     1381 ctgagtgtgc atcatcaatc attacacgtg cgccatactt tttagcaatt tcaattaagc
     1441 gtggcaattc aactatttct ccaccagtac tgaacactcc atccgacact ataagttttc
     1501 cggtttcagc aggaagtttt gaaatagttt tttctaaatc atccatatcg ttgtgtttgt
     1561 atctaacaag accgcccatt gctccttttg tcattaaagt tccggcaacg atacaagcat
     1621 gattgtcttt gtctgagatg acaaaatcat ttctttgaac taaagttgga ataacaccct
     1681 gggctgattg ataacctgtg ctgaaaagta gaactgcctc tgtgttaaag aattttgcta
     1741 atcttctttc caattcaata tgtagatcta gtgtcccggt taagtatcgg gaacctgaac
     1801 aaccggtacc atatttttca atcgctttaa ttgcagcttc tttaacctgc ggatggcctg
     1861 ttaagccaag gtagttgtta gaacctgcca ttataattct cttaccttct atctgaacaa
     1921 ctggtccctc atttgcttcg attggtctga agtaagggta aacacccata gctttaatct
     1981 catctgcgcg tgtaaactcc tgacacttct taaagatatc caaaaaatcc tcctgaactg
     2041 atgtgtatta agaaaactga ttcgactttt tattaaattg cgatcaaaaa ttatagctaa
     2101 ttacccataa aaacaaataa acatcatata tgcaaactca agaaatagca ctagttacgg
     2161 gcgcaaatgg ctttgtagga agccaccttg ttgataatct tttgactaaa ggattaaagg
     2221 ttagatgcct tgtaagaaaa tccagcaaat taaagtggct ggataaaaag gatgttgaaa
     2281 tctttgatac cggtttattt gataaagatg gactaaggaa agcctttaag gatgtaaatt
     2341 atatttttca tgttgcaggg gttgtaaaat caaaaacaaa ggagggttac tttaaaggaa
     2401 atgtggagac tacaaggaat ctgcttgaag tagctctcga aaactcatca atcataaaaa
     2461 gattcctggt ggtaagcagt cagactgttt ctggtccctc tactgaaggt aatcctgtta
     2521 atgaaaatac cgaatgcaac ccaattacta cttacggcaa aagtaaactg gaagaagaaa
     2581 agttagtgct ttcatataaa gataaattac ctatcactat ctgtcgtgct ccggcagttt
     2641 atggtgaaag ggataccgaa atttttattt actttcagac ttttagcaaa ggattaacaa
     2701 caacaattgg atttaacaaa aaagaattaa gtctaattca cgctgttgat ttagctgaag
     2761 gattttatct tgcggcaatg agtgaaaaag caaaaggtga aatttatttt ataggttctg
     2821 aaaaatatta tacctggcag gaaattaatt ccatcacttc aaaaacactt aataaaaaac
     2881 cgattataat taaagtacct cactttttag tttatacaat agctgcagtt gcacaattct
     2941 ttgcaatgtt cagcagcaaa ccggctactt taaatattga aaaagcaaaa gatattactc
     3001 agcaatactg gacctgtgat acttcaaaag caataagaga attggggtac agacagaatg
     3061 tttctattga ggcgggtatt aaaagaacct gtgattggta caaacaaatg aagtggatct
     3121 aaaaaattca atgtagctaa aactatatcg atcataacgg ctaagaaata tttatttgta
     3181 tttgagaatg aatttgtaaa ttaacttatg caaaaaagta aagttctcgt tatagatgat
     3241 gagattgatt taagagacct tattaaaagg ttacttgaac tggaaggcta tacggtcttt
     3301 actgctgaaa atgccgaaca gggactgaat gtaataaaga gtaatgagat agatattgtt
     3361 ttatcagatg tacgcctgcc tgacaaaaat ggacttgaat taatcccact gatcaaaaaa
     3421 atccatccat caattgagat tatcatttta acggcttatg gaaaaattga agatggtgtt
     3481 agagccatca aagatggtgc attcgattat atagtaaaag gggatgagga taacaaactt
     3541 ctactcgtaa taaaaaatgc aaacgataaa attcaaatgt ttaatcgtat ttctcatctt
     3601 gaaaaaaata ttttttctaa atatacattt gataacataa caagtaaatc tccccttata
     3661 caagaagcaa taagtgttgc aaaaaaagcg gcaaattctg caagccctat attattagtt
     3721 ggtgaaaccg gtactggtaa ggaagttttc gcacaatcta tacactatga aagtgagaga
     3781 aggaaagaac cgtttgttgc gattaactgc agcggatttg ctaaagatct tttagaatcc
     3841 gagatgtttg gatacaaagc cggggctttt acaggtgctg ttaaaaataa aaaaggattg
     3901 ttcgaagaag ctgatagagg aactttattc cttgatgaaa tcggggatat ggatatttca
     3961 cttcaggcaa aattattgcg cgtcttggaa aataatactt ttataaaaca aggtgatact
     4021 aaagagtctt ctgttgatgt aagaattatt gctgcgacta ata
//
LOCUS       DASWBY010000619         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_210617, whole genome shotgun sequence.
ACCESSION   DASWBY010000619 DASWBY010000000
VERSION     DASWBY010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210617"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VF870_11280"
     CDS             <1..1060
                     /locus_tag="VF870_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070005 - cysteine-type aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEX9252816.1"
                     /translation="ESFKLDFTGMNYPQSKEEFKYYWHNDPVSQGQTGTCWSFSTTSF
                     FESEVYRLTGKKVMLSPIFTAYWEYVEKVKEYVRTRGKSIVDEGSEANAVTRIYKQYG
                     IVPLESYTGLLPDQKFHDHSKMIDEIRDYLKNIKATNAWDEDEVVRVIKSIMNFYLGV
                     PPTEVIVDGKKYTPKEYLTNYLKLNTDDYVDVLSYMQQPYWEQVEYEVPDNWWHNKDY
                     YNVPLDDFMNIIKSAIKNGYTMAIGGDVSEAGYDSWTKCAVVPTFDIPSDYIDENSRQ
                     FRFSNETTTDDHGIHLVGYEELDGKTWFLIKDSGAGSHNTGDKGYYFYNEDYVKLKIM
                     DFMVHKDMFKEYLTKFNK"
     gene            1181..>2784
                     /gene="gatE"
                     /locus_tag="VF870_11285"
     CDS             1181..>2784
                     /gene="gatE"
                     /locus_tag="VF870_11285"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HEX9252817.1"
                     /translation="MYDFIFKPFNEMTEDDYVKVGFKSGLEIHQQLLTEKKLFCRCPA
                     GKYSKEYDAEILRHMRPTLSEMGVYDGTALMEFKTKKNIIYHIKRETVCTYEMDDTPP
                     FLINEEALDIAIGIGLLYNCNIVDELHIARKQYLDGSIPTGFQRTAIYALDGWIPYKD
                     RKVKIVQMSIEEDSCREVSDIGHERVYLTDRLGMPLIETVTEPAMRTPQEAAEVAWQC
                     SKLVRSTLKVRRGMGAAREDVNVSVEGGTRVEIKGVPQITLIPLLTYNEAMRQWNLLR
                     LREELKKRGITPETFKAESHDITKLLKKVHYAPLKSAADSGMKLSAVVLKGFKDLLHW
                     QTQTDTYFSKEISDRVRVIACLTNIPNLIHSDSKSDTVASSDWQKVKKFTNADDNDTM
                     IIVWGSEDDVTTAVKEIIIRAKEATIGIPSETRQAMSDGTNGFERILPGADRMYPDTD
                     LPPKKITKERLDKIESWLPEQFWKRIEWYKSLKIPIDTIEELSISPYANIFKKAVNDW
                     KINPTTAAVFLIQYPKRLKKHGVTTE"
BASE COUNT          991 a          447 c          559 g          787 t
ORIGIN      
        1 ggaaagtttt aaactagatt tcacggggat gaattatccc caatcaaaag aagagtttaa
       61 atattactgg cataacgatc cggtttcgca aggacaaaca ggaacttgct ggtccttttc
      121 tactacatca tttttcgaat ccgaagttta tcgtttaaca gggaaaaaag ttatgctttc
      181 tccaatcttt actgcttatt gggaatatgt tgaaaaagtg aaagagtacg ttcgtacaag
      241 aggcaaatct attgttgatg aaggttctga ggcaaatgcg gttacaagaa tttataaaca
      301 atatggaatt gttccacttg aatcttatac cggattattg cctgatcaaa aatttcatga
      361 tcacagtaaa atgattgatg aaatacgtga ttatttaaag aacataaaag cgacaaatgc
      421 ctgggatgaa gatgaagttg tcagagttat aaaatcaatt atgaattttt atctcggagt
      481 tcctccaaca gaagttattg ttgatggaaa gaaatacacg ccaaaagaat atctgacaaa
      541 ttatctaaaa ttaaatactg atgattatgt cgatgttctt tcatatatgc agcagccgta
      601 ttgggaacag gttgagtatg aagttccaga taactggtgg cataacaaag attattataa
      661 cgttcctctt gatgatttta tgaatataat taaaagcgca attaagaatg gttatacaat
      721 ggcaatcggg ggagatgtta gtgaggctgg ttatgatagc tggacaaagt gtgctgtggt
      781 tccaacgttt gatattccat ctgattacat cgatgaaaac tccagacaat tccgttttag
      841 taacgaaaca acaactgatg atcatggtat tcatcttgtt ggatatgaag agttagatgg
      901 aaaaacatgg tttttaataa aagattccgg ggcgggctcc cataacacag gcgacaaagg
      961 ttattacttc tacaacgaag attatgttaa gttaaagatt atggacttta tggttcataa
     1021 agatatgttt aaggaatatt taacaaagtt caataaatag ataaagacaa tggtacgctg
     1081 atcgttatga tattttataa tttgaactga tcataaataa tcataatcat cataaaaatc
     1141 agtgtatcct ttattaaaac aataactcag cggctcatat atgtacgatt tcatcttcaa
     1201 gcctttcaac gaaatgacag aggacgacta tgtaaaagtc ggattcaaat ctggattaga
     1261 aattcatcaa cagcttttaa cagagaaaaa acttttctgc cgctgtcctg ccggaaaata
     1321 cagcaaagaa tacgatgcag aaatacttcg tcatatgcgt cccacacttt ctgaaatggg
     1381 agtttatgat ggtacagcgt tgatggaatt taagactaag aaaaatatta tttatcatat
     1441 caaacgggaa accgtttgta cttacgaaat ggatgatact ccaccattct taattaacga
     1501 agaagcactt gatatcgcaa tcggaattgg attgctttat aattgcaata ttgtagatga
     1561 acttcacatc gccagaaaac aatatttaga tggaagcatt ccaacgggat ttcagcgaac
     1621 agcgatttat gctctagatg gttggattcc ttacaaagac agaaaagtaa aaattgttca
     1681 gatgtctatc gaagaagatt cctgcagaga agtttctgat atcggacacg aaagagttta
     1741 tctaacagac agacttggaa tgccgcttat cgaaacggta acagaaccgg caatgcgcac
     1801 tccgcaggaa gctgctgaag ttgcatggca atgtagtaaa cttgtaagaa gtactctaaa
     1861 ggtacgccgc ggaatgggcg ctgcacgcga agatgttaac gttagtgttg aaggtggaac
     1921 ccgagttgaa ataaaaggag tccctcaaat aacgttaatt ccgcttctta cttataacga
     1981 agcgatgcgg caatggaatc ttttgcgatt aagagaagaa ttaaagaaac gcggaattac
     2041 tcctgagact tttaaagcag aatctcatga catcacaaaa cttttaaaga aagttcatta
     2101 tgcacctcta aaaagtgcgg cagattcagg aatgaagcta agtgcagttg tgttgaaagg
     2161 ttttaaagat ttgcttcact ggcaaacaca aacagataca tatttctcaa aagaaatatc
     2221 tgatcgtgtt cgggtgattg catgcttaac taatattcca aacctaattc attctgacag
     2281 caagagcgat actgttgctt cttcagattg gcagaaagta aagaaattta ccaacgctga
     2341 tgataacgat actatgataa ttgtttgggg gagtgaagat gatgttacaa cagcagtgaa
     2401 ggagattata attcgtgcta aagaagcaac aattggaatt ccctctgaaa cacgacaggc
     2461 aatgagtgat gggacaaatg gatttgaaag aattttacct ggtgccgata gaatgtatcc
     2521 cgatacagat cttccgccaa aaaaaattac aaaagaaaga ttggataaaa ttgaatcctg
     2581 gctgcctgaa caattctgga aacgaataga atggtacaaa tctttaaaaa tacctataga
     2641 cacaatagaa gaactttcaa tttctcctta cgcaaatatt tttaagaaag cagtaaatga
     2701 ttggaaaata aatccaacca ctgcggcagt atttctcatc cagtatccaa agagattaaa
     2761 aaagcacggg gttacaacgg agtg
//
LOCUS       DASWBY010000620         4897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_704030, whole genome shotgun sequence.
ACCESSION   DASWBY010000620 DASWBY010000000
VERSION     DASWBY010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704030"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VF870_11290"
     CDS             <1..715
                     /locus_tag="VF870_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252818.1"
                     /translation="RLKTRIEYNHFFIKDTGLKLKVPYGVLNIYYDIYKFPYRTNENS
                     LSSEGNDLLIDFVSMPLPKFEVRLRYKYENKEVTELINIDKEIVKRLKQIARTEFIYN
                     LSIELRLKTRIEYNHFFIKDTGLKENGFLVFQDVRYVPLKNINLYGRIIFFQTDSFNS
                     AIYEYENDLLGVMPNLAMYGKGVRWYLIVKYKPLQFLTLSTKYSETYKPDETSLSSGD
                     NEIIGNVDNRFSFQIDISF"
     gene            complement(832..2058)
                     /locus_tag="VF870_11295"
     CDS             complement(832..2058)
                     /locus_tag="VF870_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9252819.1"
                     /translation="MKINQILAISLQSLKSNRLRTALTILGVVVGIFSIIVIMTILSM
                     LQSSIDEGLSSLGKNTFQIQRDDNVFGGGPRMHTRHRKDLTIEEADRLRELLKQAKYV
                     GVEQWQFGKVVKFGNKETNPNISIAGVNTDAMRTNDWNVEEGRDFRVDDINYSKNVCL
                     IFQPIVQKLFPNSNPIGQEIRVDGRPLKVVGIVEPQTALFGSSRDSYVIVPITTWQSM
                     YGRYGRSVNITVMSYSKTDYNDVIEAATGHMRTIRKDAPGEPNSFYIFSNESMIEQTN
                     QITEPIKLIALAISLIALIAAGVGIMNIMLVSVTERTREIGIRKALGARKLWILLQFL
                     FEAIILCLLGGLVGIIMGVSIGNIAGSFLNAQSAVPWDWVLIGLTMCVAVGVIFGTYP
                     AYKASNLDPIEALRYE"
     gene            complement(2058..3293)
                     /locus_tag="VF870_11300"
     CDS             complement(2058..3293)
                     /locus_tag="VF870_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9252820.1"
                     /translation="MSSIFLELSEGLAIAWRAIRANKIRAALTMLGIFIGVTAVVLMS
                     TAIKGIYNSLQNGVSALGSDVLYIDKWAWFSNEEWWKMRNRRNIDMEDYHRFKELAKL
                     PLAVAPVQNAMQTIKYADRKVENVFLNGSTSDYVKTTNFDFDLGRFYSEVESNGSRNV
                     AVIGSEISSKLFPRGDALDKTIKIGPVNFKVVGVLTEQGSTLLGAFNPDNQVFVPIGT
                     IFKYFTSRHFSSVTINVRAASPELLEDTKAEAEGVMRKIRGLSYDEPNDFTINQQEGL
                     TSFLDSITIVIKIAGLFITGLSLFVGAIGIMNIMFVSVKERTREIGLRKAIGATRRTI
                     LAQFLLESSVICLIGGLIGLIAAILLSLMLNQFFPTSIQYDVVIIAILISLLTGIISG
                     LAPAYSAAKMDPVDALRYE"
     gene            complement(3313..4035)
                     /locus_tag="VF870_11305"
     CDS             complement(3313..4035)
                     /locus_tag="VF870_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9252821.1"
                     /translation="MNIINIEHIAKIYQVGSEEVHALRDVTLAINRNEYVAIMGPSGS
                     GKSTLMNMLGCLDTPTSGIYDFKSVSVSEMTDNELARIRNKEIGFVFQTFNLLARSDA
                     VHNVELPLIYAGVPSSERKERAKQALMDVGLGDRMHHKPNELSGGQRQRVAIARALVT
                     NPAIILADEPTGNLDSKTGEEIMALFHEIHEKGNTIILVTHEEYIAEHALRIIRLKDG
                     LIEKDEIVEKRFIPKSKASFVQ"
     gene            complement(4050..>4897)
                     /locus_tag="VF870_11310"
     CDS             complement(4050..>4897)
                     /locus_tag="VF870_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9252822.1"
                     /translation="LETAKSNYLTTKASYEAAEANVLQSKASLRETLETLYKTTIVSP
                     MDGVVTKLNVELGERVFGAGFSMGTDIMTISDLRNIEAVVDVDENDVVLVSIGDTANI
                     KVDAFKDQEFTGLVSEIGNSANTTGLGSQNEVVNFEVKIKLLNPKTSLRPGMSCTAEI
                     ETETITDVLSVPIQSVTTRMEGPKEGQKMVEEGSGDDFQQVKEVKKEKITKTQEVVFL
                     IKDGKAKKVDVETGLSDDNYIAITKGLEGGEEVVSGSYKAISRDLNDGSKVRVEEKYS
                     NKPKK"
BASE COUNT         1524 a          959 c          730 g         1684 t
ORIGIN      
        1 gagattaaaa accagaattg aatacaatca tttcttcatt aaagataccg gattaaaatt
       61 gaaagtacct tatggtgttt taaatattta ttatgatata tacaaatttc cctatcgcac
      121 aaatgaaaat tcactttcca gtgagggtaa tgatttgttg atagattttg tttcaatgcc
      181 attgccaaag tttgaagtta gattacgtta caaatatgaa aacaaagagg ttacggaact
      241 gattaatatt gataaagaaa ttgtaaaaag attgaagcag attgcaagaa ccgaattcat
      301 ttataattta tccattgagt tgagattaaa aaccagaatt gaatacaatc atttcttcat
      361 taaagatacc ggattaaaag aaaatggatt tttggttttt caggatgtca gatacgttcc
      421 attaaaaaat ataaatcttt atggaagaat aattttcttt caaacagatt catttaattc
      481 tgccatttat gaatatgaga atgatttgct tggtgtaatg cctaatctgg caatgtatgg
      541 aaaaggagtt aggtggtacc tgattgtaaa atacaaacct ttacaatttc ttactctatc
      601 cacaaagtat tctgaaactt ataaaccaga tgaaacttcg ttgagttctg gagataatga
      661 gattattgga aatgtggata acagatttag ttttcagata gatataagtt tttaggaatg
      721 ttaattatac ttcaaaagtc tttgaatata attcataaaa gtcatttcgc atgagtcttc
      781 gagtaaacag atttccacct atggtggaaa tgactttcaa attaaaatta attattcata
      841 tctcagtgct tcaattgggt caaggtttga agctttgtaa gcaggatatg ttccaaatat
      901 aactcctact gctacgcaca tcgttaagcc aattaaaacc cagtcccaag gtaccgcaga
      961 ttgtgcgtta agaaaactac cggcaatatt tcctatactg acacccataa ttattcctac
     1021 aagaccgcct aataagcaaa gtataattgc ttcaaaaagg aactgaagta aaatccataa
     1081 ttttctggcg ccaagtgctt ttctaattcc aatttcacgc gttctttctg taactgaaac
     1141 aagcataata ttcataatcc caacaccagc ggctatcaaa gcaataagtg aaattgctaa
     1201 tgcaattagt tttataggct cggtaatttg atttgtctgt tcaatcattg actcgttgct
     1261 gaaaatataa aaactatttg gttctccggg cgcatctttt cgaatggttc tcatatgacc
     1321 tgtcgccgct tcgataacat cgttataatc agttttgctg tacgacatta cagtgatatt
     1381 aacagagcgg ccatatcttc cgtacattga ttgccaggtt gtaattggaa ctattacata
     1441 actatctcta cttgaaccga agagtgcagt ttgcggttca actattccaa caacttttaa
     1501 gggccttcca tcaactctta tttcctgccc aattggatta ctattcggaa acagtttctg
     1561 aactatcggc tggaaaatca aacaaacatt tttagaataa ttaatatcat ctacacggaa
     1621 atctcttccc tcttcaacat tccaatcatt tgttctcatt gcatcagtgt taacacctgc
     1681 aatagatata tttggattgg tttccttgtt tccaaatttg actactttcc caaattgcca
     1741 ctgttcaaca cccacatatt tggcttgttt taataattcc cttaatcgat cggcttcttc
     1801 aatagtaagg tcttttctgt gtctagtatg catcctagga ccaccgccaa atacattatc
     1861 gtctctttgt atctgaaaag tatttttacc tagagaagat aacccttcgt caatactgct
     1921 ctgcaacata gacaggatgg tcataatgac aattatggag aagataccta caacaactcc
     1981 aagaattgtc aatgcggttc ttaatcgatt actttttaag gactggagag agatcgctaa
     2041 tatttgattt attttcatta ctcatacctc aacgcgtcaa caggatccat tttagcagct
     2101 gaatatgctg gtgccaatcc ggaaataatt cctgttaata aagaaattaa aattgcaata
     2161 atcacaacat catattggat agaagtcggg aaaaactgat taagcataag acttaacaaa
     2221 attgcagcaa ttagaccaat taatccaccg atcaaacata taacagaaga ttccagtaaa
     2281 aattgggcga gtatagttcg tcgtgttgca cctatcgctt ttctcaatcc aatctcacgg
     2341 gttctctctt taaccgaaac aaacataata ttcataatac caatcgctcc aacaaataat
     2401 gataaacccg tgataaataa tccggctatc tttattacta ttgtaattga atcaagaaaa
     2461 ctggtcaacc cttcctgttg attaattgta aagtcattgg gctcatcata agaaagccct
     2521 ctaatctttc tcatcacacc ttctgcttct gcttttgtat cttctaaaag ttcaggactt
     2581 gctgccctta catttattgt taccgagcta aaatgacgtg atgtgaaata tttgaatatt
     2641 gtgcctattg ggacgaatac ctgattatct ggattaaatg cacctaaaag tgtactgccc
     2701 tgctcagtaa gaacgccaac tactttaaag ttaacaggac caattttaat agttttatcg
     2761 agtgcatcgc ccctaggaaa taatttactg ctaatttcac ttcctatgac ggcaacattt
     2821 ctggatccgt tactttcaac ttcagaataa aatcttccta aatcaaaatc aaagtttgtc
     2881 gttttaacgt aatcagaagt ggaaccatta aggaaaacat tttcaacttt tctgtcagca
     2941 tatttaatag tttgcatagc attttgtaca ggcgctactg ctaatggaag ttttgccaat
     3001 tctttaaatc tatgatagtc ttccatatct atgttacgac gattacgcat tttccaccat
     3061 tcttcgttgg aaaaccatgc ccatttatca atgtaaagca catcagagcc aagcgcacta
     3121 acaccatttt gcaaggaatt gtatatacct tttattgcag tagacattaa tactacagca
     3181 gtgacaccaa taaatattcc aagcattgtt aatgctgcac gaattttatt tgctctaatg
     3241 gctctccatg cgattgccaa tccttcgctt aattctaaaa aaattgaaga catttttaac
     3301 tcttattatt tatcattgta caaatgaagc ttttgatttt ggaatgaagc gtttttcaac
     3361 aatttcatct ttctcaatta atccatcttt taatcgtatt attcttagag cgtgttccgc
     3421 tatgtactcc tcatgagtaa caagaataat tgtattacct ttttcgtgaa tttcatgaaa
     3481 taaagccatt atttcttcac cggtttttga atccagattt ccggttggtt catccgctaa
     3541 aataatagca ggattagtta ccaaagctct tgctatggca actctttgtc tttgaccgcc
     3601 cgataattca tttggcttgt gatgcatacg atcgccgagt cctacatcca ttaatgcttg
     3661 cttagctctt tcttttcttt cggatgatgg tacaccagca taaattaacg gaagttctac
     3721 attatgcaca gcgtcggatc ttgcaagaag attaaatgtc tgaaacacaa atccaatctc
     3781 tttatttcta attctggcaa gttcattgtc tgtcatctcg cttacactaa cacttttaaa
     3841 gtcatatatt ccagaagtag gcgtatcaag acatccgagc atattcatca atgtagattt
     3901 tcctgaacca gatggaccca taatggcaac gtactcattc ctattgattg caagagtaac
     3961 atcacgtaat gcatgaacct cttcggaacc aacttgataa atctttgcta tatgttctat
     4021 attaattatg ttcattactg cttccgaatt tatttctttg gtttattaga atacttttct
     4081 tcgaccctga cttttgatcc atcatttaag tctctagaga tagctttgta acttcctgat
     4141 acgacctctt caccgccttc taatcctttt gtaatagcta tataattatc atcactcaaa
     4201 cctgtttcaa catcaacctt tttagcttta ccatctttaa taagaaatac tacttcttga
     4261 gttttagtta ttttttcttt tttcacttct ttaacctgtt gaaagtcgtc tccgcttcct
     4321 tcttcaacca ttttttgtcc ttcctttgga ccttccattc ttgttgtaac actttgtatc
     4381 ggaacactta aaacatccgt aatagtttct gtttctattt ctgccgtaca actcatacca
     4441 ggtcttaagc tggtctttgg gttaagaagt tttattttta cttcaaaatt tacaacttca
     4501 ttctgactgc ccaatccggt tgtattagcg ctattaccaa tttcggaaac tagaccggta
     4561 aactcctgat ctttaaaagc atctacttta atattagcag tatcaccaat tgaaactaaa
     4621 acaacatcgt tttcatctac atccactaca gcttcaatat ttcttaaatc agatattgtc
     4681 ataatatcag tacccatact aaatcctgca ccaaaaactc tttcaccaag ttcaacatta
     4741 agtttggtta ctactccatc cataggtgaa acgattgtag ttttatacaa tgtttcaaga
     4801 gtttctctta aagaagcttt actttgcagt acatttgctt cggctgcttc ataagaagct
     4861 tttgtggtta agtaattcga ttttgctgtt tccaatt
//
LOCUS       DASWBY010000621         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_175442, whole genome shotgun sequence.
ACCESSION   DASWBY010000621 DASWBY010000000
VERSION     DASWBY010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175442"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VF870_11315"
     CDS             complement(<1..864)
                     /locus_tag="VF870_11315"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEX9252823.1"
                     /translation="MVRNLLFSVIIIALILPTQVGNSCTNFIVTKGASVDGSVMVSYT
                     ADSYGFYGELYHYPAAVYQNGAWLEIYEWDTGKFLGKIPQASVTYNVIGNMNEHQVVI
                     GETTFGGREELGAANGIIDYGSLMYIALQRSKTAREAIKIMTDLVNEFGYYSSGESFS
                     VADANEAWILELIGKGEGNKGAVWVAVKIPDGYISGHANQSRITTFPLNDPDNCLYAP
                     DVISFARDKEYFSGKDSEFSFSDAYAPLDFGAIRFCDARVWSLFRRCNSTMDKYISYI
                     KGESLERMPLYI"
     gene            1065..1595
                     /locus_tag="VF870_11320"
     CDS             1065..1595
                     /locus_tag="VF870_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252824.1"
                     /translation="MKILLMIVSLTFAQLIQAQEIEVVKPHYDIFSFNKEVTLPGTPE
                     QIYDAATGDISGWWDHSFSGNPKKFFIEAKPGGGFYEIFDDEGNGVLHATVIYSKRPE
                     MIRFDGPLGLSGRAVQVVTTYEFSEVGKDSTLMKVSVHGSGEMEEGMPAIVEKVWEHF
                     IFERFKPYIESGKHLK"
     gene            1662..2036
                     /locus_tag="VF870_11325"
     CDS             1662..2036
                     /locus_tag="VF870_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX9252825.1"
                     /translation="MNCPACKNPMVILELNQVEIDYCSVCKGIWLDRGELDLIFSVKE
                     KKEISELFSVKNDFDETKRRCPICKKKMDKVEFENTGIIIDRCINNHGLWFDSGELKS
                     LLKTAEEKNSKIIDHLKEMFGE"
     gene            2092..2649
                     /locus_tag="VF870_11330"
     CDS             2092..2649
                     /locus_tag="VF870_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX9252826.1"
                     /translation="MAFLLIFIAIIVIIGFYFVSIYNSLVGLRNRVKNAWSQIDVQLK
                     RRHDLIPNLLETVKGYMKHEREIMENITKYRSQAMDANTVGEKAAAEGMLSGALGQLR
                     VQVENYPDLKANQNFLALQEELTSTENKISFARQAYNDQVLFFNNKIQMFPSNIVAGM
                     FSFKEEEFFEIEDKTEKAVPKVSFN"
     gene            2668..3882
                     /locus_tag="VF870_11335"
     CDS             2668..3882
                     /locus_tag="VF870_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9252827.1"
                     /translation="MMWELIVANRRKSTFIFIGMGLLLILIGFTFGSFYAPDNGGFIG
                     VFFALILWGILSMVSYFAGSKILLAVSGAKEVTQDVHPQLYNVVEEMRIAANLPNQPK
                     IYIISDAAPNAFATGIKPENSAIAVTAGLLGTLNRDELQGVVAHEMSHIMNRDVLLMT
                     FAGMMLGAITLMAEVFARSLWFGGGSRYKSKSSDRGGQAQLIIMVLAVVLAILGPIMA
                     QLLYFAISRKREYLADASAVRLTRYPDGLASALEKISNTNLDLKTANKVTAPMYIINP
                     LKKKGMQIANLTSTHPPITERIKILRSMTQGVNYANYQNAFNTVKGRSSGLIPQSGLT
                     DSTKIGLRSSELSQTSFETVKQVKREVGDLMMKLNDFIFINCTCGLKLKVPPDFKDAS
                     ITCPRCGRIHNL"
BASE COUNT         1375 a          654 c          752 g         1340 t
ORIGIN      
        1 gatgtataaa ggcattcttt ctaaagattc accttttatg tatgatatat atttatccat
       61 agtagagttg catcttctaa atagtgacca aactcttgca tcacaaaatc ttattgcacc
      121 aaaatccaat ggggcataag catcggaaaa actaaattca gaatcttttc ctgaaaagta
      181 ttctttatct cttgcaaatg aaattacatc cggggcatat aaacaattat caggatcatt
      241 taatggaaaa gttgtaatgc gtgattgatt agcatgaccg gaaatgtatc catcaggaat
      301 ttttacggca acccaaactg cacctttgtt tccttcgcct ttaccaatta attccagaat
      361 ccatgcttca tttgcatcag caactgaaaa agattcgccg ctgctgtaat acccaaattc
      421 atttaccagg tcagtcataa ttttaattgc ttcgcgagca gttttacttc tttgtaatgc
      481 aatgtacatt aaactgccat agtcaattat tccatttgct gctccaagtt cttctctgcc
      541 gccaaaagtt gtttcgccaa ttacaacctg atgttcattc atgtttccta ttacgttata
      601 agtaactgaa gcttgaggaa tttttccaag aaattttcct gtatcccatt catatatttc
      661 taaccatgct ccgttctgat atacagcggc aggataatga taaagttcac cataaaaacc
      721 atatgaatca gctgtgtaag aaaccataac ggaaccatcc acacttgcac ctttggtcac
      781 aataaaattt gtgcatgaat ttcctacctg tgttggtaag atcaaagcaa taataataac
      841 actgaaaagg agatttctaa ccataattca tccaatttaa attatctaaa aaatattgct
      901 gaaaagataa ggaaataggt ttgatttggg aatgaaaatc gtataacact aaaaaaaata
      961 agtgtaatta catattaaga ttattgattt ttcagattat catttatttt ataactgttt
     1021 gatcttgtaa agtttatttt atctatcatt ttaaggaggc aataatgaaa attttactaa
     1081 tgattgtgtc actgactttt gcacaattaa ttcaggcaca ggaaatcgaa gttgtaaaac
     1141 ctcattatga tatattttca tttaataaag aagtaactct tccagggact ccagagcaaa
     1201 tttatgatgc agcaacaggc gatattagtg ggtggtggga tcattcgttc tctggcaacc
     1261 ccaaaaagtt ttttatagaa gcaaaacccg gtggcgggtt ttatgaaatt tttgatgatg
     1321 agggaaatgg agtattacac gcgacagtta tttactctaa acggccagag atgattagat
     1381 ttgatggacc acttggctta tctggaaggg ctgtgcaagt tgtaacaacg tatgaatttt
     1441 ctgaagttgg aaaagattca actttgatga aagtttctgt tcatggttcc ggagaaatgg
     1501 aagaaggaat gcctgcaatt gtagaaaaag tttgggaaca ttttatcttt gaaagattta
     1561 aaccttatat tgaatctggg aagcatttga aatagtttca tcaatactat ttttaattac
     1621 agatattctt tgcaatttca cagcaataaa atattaaaat tatgaattgt cccgcttgta
     1681 aaaatccaat ggttatttta gaacttaatc aggttgagat tgattattgt tccgtgtgta
     1741 aaggtatctg gctggatagg ggagaattag atctgatatt ttcagtaaaa gaaaaaaaag
     1801 aaatatcaga attattttct gttaaaaatg attttgatga aaccaaaaga agatgtccta
     1861 tctgtaaaaa gaaaatggat aaagttgaat ttgaaaatac tgggattata attgatagat
     1921 gcataaacaa tcatggactt tggtttgata gtggagaatt gaaatctttg ctaaaaaccg
     1981 ctgaagaaaa gaacagtaaa ataattgatc atttaaaaga aatgtttgga gaataaagta
     2041 aattcagttt attaaaaacg atatttttta ttatacacta acggaggcat tatggctttc
     2101 ttactaattt tcattgcgat aattgttatt atcggatttt acttcgtatc tatatataac
     2161 tcattggttg gattaagaaa ccgtgttaaa aatgcatggt cacaaattga cgtacaattg
     2221 aaaagaagac acgatcttat tcccaattta cttgaaactg taaaaggata tatgaagcat
     2281 gagcgtgaaa taatggaaaa cattaccaag taccgcagtc aggcgatgga tgcaaatact
     2341 gtgggtgaaa aagctgctgc tgaaggtatg ttaagtggtg cccttggtca gcttcgtgtg
     2401 caggttgaaa actatcctga tctgaaagca aatcaaaact ttcttgcatt gcaggaagaa
     2461 ttaacatcaa ctgaaaacaa aatttctttt gcgcgccagg catataatga tcaggtttta
     2521 ttttttaaca ataaaattca gatgtttcca tcaaacattg ttgcaggaat gttcagcttt
     2581 aaagaagaag aattctttga gattgaagac aagactgaaa aagcagttcc taaagtcagt
     2641 tttaattaat ccaggatatt tttctttatg atgtgggaac taattgtagc taacagaaga
     2701 aagtctactt ttatttttat cgggatggga ttacttctca tcctgattgg ttttactttt
     2761 ggaagctttt atgcacctga taatggcgga ttcattggag ttttttttgc attaatactg
     2821 tggggaattc tatcgatggt aagttatttt gcaggcagta aaattcttct ggcggttagc
     2881 ggtgcaaaag aagttacgca ggatgttcat ccacaacttt acaatgttgt tgaggaaatg
     2941 agaattgctg ctaaccttcc taatcaaccc aaaatttata tcatatcaga tgcagctcct
     3001 aatgcttttg caaccggaat taaaccagaa aattctgcga ttgctgtaac tgccggttta
     3061 cttggcacac ttaatcgtga tgaacttcaa ggtgttgttg ctcatgaaat gtcacacatt
     3121 atgaaccgcg atgttttgct aatgacattt gcgggaatga tgctcggggc aattacattg
     3181 atggctgaag tatttgcaag aagtttatgg tttggcggtg gttcgcgtta caaatcaaaa
     3241 tcgtctgata gaggtggtca ggcacagcta attattatgg tgctagcagt agtattagca
     3301 attcttggtc caattatggc acagcttctt tattttgcta tttcccgtaa aagagaatat
     3361 ctggcagatg catccgcggt tagattaaca cggtacccgg atggtttggc atcagcactt
     3421 gaaaaaattt caaatactaa tcttgatttg aaaactgcaa acaaagtaac agctccgatg
     3481 tatatcatta atccattaaa gaaaaaagga atgcagattg caaatctgac aagcacacat
     3541 cctccaatta ctgaaaggat taaaatatta cgttcgatga cacagggagt caattatgca
     3601 aattaccaga atgcttttaa tactgttaag ggtagaagtt cgggtttaat cccacaatcg
     3661 ggattaacag attcaacaaa aattggttta agaagttctg aactttctca aacatcattt
     3721 gaaacagtta aacaagttaa aagagaagtt ggcgatttga tgatgaaatt gaatgatttc
     3781 atttttatta attgtacttg cgggcttaag ttaaaagtgc ctccggattt taaagatgct
     3841 tcaatcacat gtcccaggtg tggacgaata cataatcttt aaactgtttt aatctttgtt
     3901 gccaaggcct ttgatacaaa tcataattaa tgtcgaatca tctggttttc aacgaatcac
     3961 atattagaat ctattcaaaa atagtttcgt tttcctgccc gcaatacatt attaatgttt
     4021 ataaccttca atctgtgtaa tacaattacg acagaaagta tttcccttga tatctacttc
     4081 ctcgatacca ggtgatgaat gcattacgca atcccaatcc a
//
LOCUS       DASWBY010000622         5374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_137494, whole genome shotgun sequence.
ACCESSION   DASWBY010000622 DASWBY010000000
VERSION     DASWBY010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137494"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VF870_11340"
     CDS             <1..568
                     /locus_tag="VF870_11340"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HEX9252828.1"
                     /translation="IAGVDSAAKWKNVLQVHSDFAGRQLELNMPVYISEAGDNLRNQA
                     IAHLLFAYGRMYFDPVQATDIYTKQCAINVSAKDAGIMAATLANGGVNPVTKKKVVSP
                     ETVMYTLPVMSTAGLYDDSGIWLYNAGVPAKSGVGGCLIAVVPGKFGIAVVSPPLDKA
                     GNSVKGQLAIKYIIQQLKVDPYLIQPKM"
     gene            707..2092
                     /locus_tag="VF870_11345"
     CDS             707..2092
                     /locus_tag="VF870_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252829.1"
                     /translation="MKNLFHLLNYKVFPFLFISLCFIFLFSGKGLAQDEGPVTAFDSI
                     EVEGTYVGIVEDRAFENTTAGEFTPGKGFTIFKNNLASLNISVYGLARYINQMPGNQE
                     FEDHLGRTRIADTRNDIWWHRTFVWLSGFFYTPKLRYTISVWGLAATNQVLIFGNLQY
                     AVNKGLTLGVGIVPNVGSRSMQGPWPFFLSSDRLLAEEFFRPGFTAGAWLTGEPLPKF
                     RYWAMVGNNLSQLGITASQMSRDLSTGLSVWWMPTTGEFGPRGGYGDFELHKELATRF
                     GLSFVHCREDRQSQVGQASAETQVKISDGLLLFETNTLADGVTVQKANFDQGAIDAGI
                     KINGWHFQIEYYFRNLSNFDLAVTDPSFDVTTVPSSIFDHGFYALASYEIIPKALQIY
                     GATSWIYDDFKRKPWDIVAGLNWYPAGSRSLRINLHGIYVDKSPASSSFGFYINGQTG
                     PTISSGVDFLF"
     gene            2120..3205
                     /locus_tag="VF870_11350"
     CDS             2120..3205
                     /locus_tag="VF870_11350"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9252830.1"
                     /translation="MKIKSSLKSLKIFYSLLLLIVLSTFTITYAQSSDKYLFNDSHFH
                     ITNYIQEGIEMEFYVDSIMGDKIGRSTVFGLPVQQQWSYRVTGENAPTYYLDTDAPLY
                     YYSFCDAWIAMQYKNLPKEKQKRLDPMIIGFNVTDMYAADHIKRVLTTFPGVFTGIGE
                     FSIHKEFVSAKISGDVASLTDPALDRIFDLCAETGLISIIHNDIDNPFPKPGKPNYVT
                     GFQDLIKRHPDAIIIWAHLGLGRIIKPIENMGDLFLDMLADPAYNNLYFDLSWDETAK
                     WLMSSDKAIDKTAEVISKYPDRFLFGTDNVAPDKQEKQLYVYHLYDRLWKSVGEEVTY
                     KVCKGNYIKLFDDARTKVRAWEKANVK"
     gene            3650..5131
                     /locus_tag="VF870_11355"
     CDS             3650..5131
                     /locus_tag="VF870_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252831.1"
                     /translation="MKTKILVSFLMLLAISLEAQDRHYWDQAVGGRTALLGGIAVGGV
                     NDYSATFYNPGALSFLSKNSMNFNFNMYGIKDFSFINGGGPGIDSRYTRVSLYPASLA
                     GSLPFLGDSLNRFSYMIYSTGYSYLRISERYEGYSDVIPTRPPSQPGLPDAFAGDEFL
                     INQGKLDALLSEVSVAFGYSKKISDNVGIGFTLIGAYRDQTKIRYETYSAFDTTYQRT
                     ATTDLYVDLDYWAVRFSGKFGISAVWDDLKLGATITTPSLAIKRASGGTDHASLTSNN
                     VFVIIDSTNNQVTQVDLLASDRQEGLPVNYKSPFSISAGIEYTFSEETKIHFAAEWFA
                     PQSTYAVMQPESSDFTRNNNVPAKYLYDSSELLKVYDSKVGVFNLGIAVEHQLNKKIR
                     GYAAIRTDFSNANYEEIKGLSVGFTDFNIYHFTAGVSTEVDNTFIAVGLEYSHGQRSD
                     FQQIFNFPSGVAEPTDLIIPLNRGTCEAIYNNFNLFFGVTQML"
BASE COUNT         1729 a          899 c         1033 g         1713 t
ORIGIN      
        1 aattgctggc gttgattcag ctgcaaaatg gaaaaatgta ctgcaagtgc atagtgattt
       61 tgcaggaaga caactggaat tgaatatgcc ggtttacata agcgaagcag gagataattt
      121 acgtaaccag gcaattgcac atttactttt tgcctatggc agaatgtatt ttgatccggt
      181 tcaggcaacg gacatttaca caaagcaatg tgctataaat gttagtgcaa aagatgccgg
      241 aattatggca gctactttgg caaatggcgg tgttaatcct gttacaaaga aaaaagttgt
      301 atcaccggaa acagttatgt acactcttcc tgtaatgtca actgccggac tttacgacga
      361 ttctggtatc tggttatata atgcaggcgt tccggcaaaa agcggtgtag gcggatgctt
      421 aatagcagta gttcctggta aatttggtat tgctgttgtt tctcctcctc ttgataaagc
      481 aggtaacagt gttaaaggtc aattagcaat caaatatatt atacagcagc tgaaagtcga
      541 tccttatttg attcaaccga aaatgtaata tttattaaaa ttttatttta gagaattgat
      601 ccgtttattc aattaagcct cctcatctca gaggaggttt atattttgat attcattaat
      661 ttaccagcca aatattttat tattatgtgt tgaaaggtat cgcgatatga aaaatctatt
      721 tcatttacta aactataaag tatttccatt tttatttatt tccttgtgct tcattttttt
      781 attctccggg aagggtttag ctcaggatga aggaccggta acagcattcg atagtattga
      841 agtagaaggt acatatgtag gtattgtcga ggacagagct tttgaaaata ctacggcagg
      901 agaatttaca cccggaaaag gttttactat tttcaaaaat aatctcgcaa gtcttaacat
      961 aagtgtatac ggactagcac ggtacattaa tcagatgcca ggtaatcagg aatttgaaga
     1021 ccatctcggg cgaacaagaa tagcagatac acgtaacgat atttggtggc atagaacctt
     1081 tgtttggctt tctggatttt tctatacccc taaactcaga tatacaatat ctgtatgggg
     1141 tttagctgct actaaccagg ttttaatttt cggaaattta caatatgcag taaataaagg
     1201 actcactctt ggtgttggaa tagttcctaa cgttgggtca agatccatgc aaggaccatg
     1261 gccgttcttt ttatcaagtg acaggttact ggctgaagaa tttttcagac cgggatttac
     1321 agcaggggcc tggttaaccg gtgaacctct acccaaattc agatattggg ctatggttgg
     1381 taataacttg agtcagcttg ggataactgc aagtcaaatg tcccgtgacc taagtacagg
     1441 actcagtgtg tggtggatgc caactactgg tgaattcgga ccaagaggcg gttatggtga
     1501 ttttgagctt cacaaagaac tagcaactag atttggtctt tcatttgttc actgccgtga
     1561 agaccgtcag tcacaggtcg gccaggcttc tgctgaaacg caagttaaaa tatcggatgg
     1621 tttattatta tttgaaacca atacacttgc tgatggtgta acagttcaaa aagcaaattt
     1681 tgatcagggt gcaattgatg caggtattaa aataaatgga tggcatttcc agattgaata
     1741 ttatttcaga aatctttcca actttgatct tgctgttact gatccttcct ttgatgttac
     1801 gactgtgcct tcgtccattt ttgatcacgg cttttatgct ttagcctctt atgaaataat
     1861 tccaaaagcc ttacagatct acggggctac atcctggata tatgatgact tcaaaagaaa
     1921 accgtgggat attgtagctg gcttaaactg gtatcctgct ggctcaagaa gtttgaggat
     1981 aaacttgcac ggaatttatg ttgataaatc tcctgcatca tcctcttttg gattttatat
     2041 aaatggtcaa accggaccaa caatttcaag cggagtagat ttcctattct gatttagata
     2101 aaaaaaggag aacttactta tgaaaattaa atcttcgcta aaatcattaa aaatatttta
     2161 ttcactattg ttgctgatag tactcagtac ttttacaatt acttacgccc aatccagcga
     2221 taagtattta tttaacgact cccatttcca cataacaaat tacatccagg aaggtattga
     2281 gatggagttt tatgtggatt caattatggg cgacaagatc ggacgctcaa ctgtgtttgg
     2341 tcttccggta caacagcaat ggtcgtaccg tgtaacgggg gaaaacgcac caacttacta
     2401 tctcgataca gatgcaccat tatactatta ttcattctgt gacgcatgga ttgcaatgca
     2461 gtataaaaat cttccaaaag aaaaacagaa acgtctagac ccaatgatta ttggttttaa
     2521 tgttaccgat atgtacgcag cagatcatat taaaagagtt ctaacaacct ttcccggagt
     2581 atttacaggt attggtgagt tcagtattca caaagaattt gtatcggcta aaatatccgg
     2641 tgatgtagca agtctcactg atcctgcgct tgatagaatt tttgacttat gtgcagaaac
     2701 aggtctgatc tcaataattc ataatgatat tgataatcct ttcccgaaac cgggcaagcc
     2761 gaattatgta accggatttc aggatttaat aaaaagacat cctgacgcaa taataatttg
     2821 ggcacatctc gggttaggaa ggattattaa gccaattgaa aatatgggag atctgttttt
     2881 agacatgctt gcagatccgg cctataataa tttatacttt gatctttcat gggatgaaac
     2941 tgccaaatgg ttaatgagtt ctgataaagc tattgataaa actgccgaag taatcagtaa
     3001 gtatcctgat agatttttat tcggtacaga taacgttgcc cccgataaac aggaaaaaca
     3061 gctctatgtt tatcatctat atgacagact ttggaaatcg gtcggtgagg aagttacata
     3121 caaagtatgc aaaggaaact atataaaatt gtttgatgat gcccgcacaa aagtcagagc
     3181 ctgggagaaa gcaaatgtaa aatgagttat atataaaata attattaatc tattacaata
     3241 tttttatcta taatgataaa tattgcgata gaaatatcag gcaacgatta aatagttaat
     3301 caattttaat tagaatgcta caatgtcgaa atatttgtaa acgatatttc tagttttatt
     3361 tactctaaaa gtgatattta caatttaaat tattttgagc atctataaaa aacttgtggt
     3421 ataactttaa ttagctccaa cattaaccgt tcttaataga gtcaagctta ataataatta
     3481 atcagcaggc atagattcct aaaaaagttc gttttgggat caggcaactt ataaaggaat
     3541 aaatggtgac ttatattaaa tgaatattgc taaaaatatt atttattaaa gtgatattta
     3601 attgctattt ttattattgc tattaataaa atcttttata aagcctgtta tgaagacaaa
     3661 aatacttgtt agctttttaa tgttacttgc tatttcactt gaagctcagg acagacacta
     3721 ctgggatcag gcggttggag gaagaacagc tttattaggc ggaattgccg tgggaggcgt
     3781 aaatgactac agcgccactt tttataatcc gggtgcctta agctttttaa gtaagaacag
     3841 tatgaatttt aattttaata tgtacggtat aaaagatttt tcatttatta atggcggggg
     3901 acctggaatt gattccagat acacccgagt atcattatat cctgcatcat tagcaggttc
     3961 tttaccgttt ttgggtgatt ctcttaaccg atttagttat atgatctata gtaccggcta
     4021 ttcatattta agaatttctg aaagatacga aggatattca gatgtgattc caacaagacc
     4081 tccatcacag cccgggcttc ctgatgcatt tgcaggtgat gaatttttaa ttaaccaggg
     4141 aaagcttgat gctttacttt ctgaagtaag tgtagctttt ggatactcaa aaaagatttc
     4201 tgacaatgtt ggaattggtt ttactttaat aggcgcatac cgtgatcaaa ccaaaatccg
     4261 ctatgaaact tattcagcat tcgatacaac ttatcaacgt actgctacta cggatcttta
     4321 tgttgatcta gactactggg ctgttagatt ttcaggcaag tttggaatat ccgcagtatg
     4381 ggatgattta aaattagggg caacaattac aacacctagt ttagctatca aacgtgcaag
     4441 cggcggaaca gatcacgcca gcctcacatc caataatgtt tttgtaatta tagatagtac
     4501 taataatcag gttactcaag tagatttact tgcctcggat agacaggaag gattgcctgt
     4561 aaattataaa tcaccgttct caatatcagc tggtattgaa tatacttttt cagaagaaac
     4621 aaaaatacat tttgcagcag aatggtttgc gcctcaatct acttatgccg taatgcagcc
     4681 cgaaagctcc gattttacga ggaacaataa tgtcccggca aaatatctat atgacagctc
     4741 tgaattacta aaggtatacg attcgaaggt cggagtattt aacttaggaa ttgcagttga
     4801 acatcaatta aataaaaaga tcagaggata cgctgcaatc aggacggact tcagcaatgc
     4861 taattatgaa gagatcaaag gcttatcagt aggtttcaca gatttcaata tttaccactt
     4921 tactgccggt gtttctactg aagttgacaa tacatttatt gctgtcggac tggaatatag
     4981 tcatgggcaa agatcagatt tccaacaaat atttaatttt ccatccggag ttgctgagcc
     5041 tactgatctg atcatcccac ttaatcgcgg tacttgtgag gccatataca ataactttaa
     5101 tttatttttt ggtgtcactc aaatgttata atatttttct agataagttt acaatttcat
     5161 aaataataaa ttctaaaaca tattagttca atatttaaag gatagtaaga aaaatgaaca
     5221 acggtcaatt gaatcatagg gatttataca ggttaccctg gacattacct gataatgcaa
     5281 tatcatggtt agaaccaaca gcaatgtgta atttagcttg cgacggctgt tatagagaga
     5341 atcaaaaaga ttcacatcta tcaatcacag aagt
//
LOCUS       DASWBY010000623         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_361362, whole genome shotgun sequence.
ACCESSION   DASWBY010000623 DASWBY010000000
VERSION     DASWBY010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361362"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VF870_11360"
     CDS             complement(<1..553)
                     /locus_tag="VF870_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9252832.1"
                     /translation="MAEVTKVYEGKVTHRAINQLSFEVNKGEFVAVMGPSGSGKTTLL
                     NLISTIDSATSGTITIDGLRPEEMTKTELALFRRRQLGFVFQDFNLLQMLTVEENLVL
                     PLTLDGIPIDEMKRRIKELAKKLDLDQILEKRPNEISGGQAQRTAIGRALIHKPNFIL
                     ADEPTGNLDSKASRDVLELLAKVN"
     gene            complement(501..1583)
                     /locus_tag="VF870_11365"
     CDS             complement(501..1583)
                     /locus_tag="VF870_11365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9252833.1"
                     /translation="MMSFRLFVKEHAAYVIFQLIMVFFIMSLYWLDGFRNIDTGIYSF
                     VISTLLILSFLFGRYVKRYAFYHKLLSQPKQMEAALQRDGKSPEQEQVEKYLHGFYRL
                     YQNEVEALYANQNRHLQFMNQWVHQMKTPLSVAELLLQEDGDVDKKSMQEEIDRLKRG
                     LDSVLINARLDTFEQDMQIEQVQLKTLVSEVVSENKRLFITHHVYPVISIDEKYIVPT
                     DAKWMRFVIGQFITNAVKYTFEEGKKVHIHATCQQGQVLLSIQDEGIGIPAIDLPRVM
                     KAFFTGENGRKTGESTGMGLYLANEICEKLGHKMSIMSEQGKGTVVSVLFQHHEVTER
                     GVSEDDHFENGRSDQSVRGQSDASCD"
     gene            complement(1580..>1991)
                     /locus_tag="VF870_11370"
     CDS             complement(1580..>1991)
                     /locus_tag="VF870_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9252834.1"
                     /translation="DFITKPFHYEIVLAKIRSHLRRTYGEYALKQEERTIKLGKLCLY
                     LERMELHFVSLEIPLQKRECTILELLLSQSPKVVTREQLLEELWDDQAFVDENTLNVN
                     MTRVRKKLQDYGITSTIETVRGAGYRFVLSAEES"
BASE COUNT          575 a          420 c          332 g          664 t
ORIGIN      
        1 ggtttacttt tgctaacagt tcaagcacat cacgggatgc cttagaatcc aaattccctg
       61 tgggctcatc cgctaaaata aagttcggct tgtggataag tgcacggcca attgccgtac
      121 gttgcgcttg tccacccgaa atttcatttg gacgtttttc aagaatttga tccaagtcta
      181 atttcttagc taattcctta attcttcttt tcatttcgtc aattgggatt ccgtctaatg
      241 ttaagggaag caccaaattt tcttctactg taagcatttg cagtaaatta aagtcttgaa
      301 agacaaaacc gagctgtctc cgacgaaaaa gagccaactc ggttttagtc atttcttcag
      361 gacgcagtcc atcaattgta atggtaccgg aagtggccga atcgatagtt gagatcagat
      421 taagtaatgt cgttttaccg cttcctgaag gacccatcac tgcgacaaac tcacctttat
      481 tgacttcaaa acttaattga ttaatcgcac gatgcgtcac tttgccctcg tacactttgg
      541 tcacttcggc cattttcaaa atggtcatct tccgatacac ctctctctgt tacttcatga
      601 tgttgaaaca gtactgaaac aaccgttcct ttaccttgct cagacatgat gctcatttta
      661 tgaccgagtt tctcacaaat ttcattggct aaatacaaac ccattcccgt actttcacca
      721 gtcttccttc cattttcacc tgtaaaaaaa gctttcatta ctcgaggaag atcaattgct
      781 ggtatgccta ttccttcatc ttgaattgac agtaatactt gtccttgttg acaggttgcg
      841 tgaatatgta cttttttgcc ttcttcaaat gtgtatttta ctgcatttgt aatgaactga
      901 ccgatgacaa atctcatcca ttttgcatcc gttggaacga tgtatttttc atcaatcgaa
      961 atgacgggat acacgtgatg tgtaataaat aaacgtttat tttctgacac tacttctgat
     1021 actaatgttt ttaattgtac ttgttcaatt tgcatatctt gctcaaaagt atcaaggcga
     1081 gcattgatta aaacggaatc tagccctctt tttaatcgat caatttcttc ttgcatactt
     1141 tttttatcga catcaccgtc ttcttgaaga agcagttcag caactgataa tggtgttttc
     1201 atttgatgta cccattggtt cataaattgc aaatgacgat tttgattggc gtacaaggct
     1261 tctacttcat tttgatataa acgataaaac ccatgaagat atttttcaac ttgttcctgc
     1321 tcgggtgatt tcccatcgcg ctgcaatgcc gcttccattt gttttggttg agacaacagt
     1381 ttgtgataaa aagcatagcg cttcacatac cgtccaaata aaaacgataa gattaataat
     1441 gtactaataa caaaagaata tattccggta tcgatatttc taaagccatc cagccaatat
     1501 aatgacatga taaagaacac cataattagt tgaaaaatca catacgcagc atgttctttt
     1561 acaaacaagc gaaaactcat catgactcct ccgcacttaa tacaaaacga tagcctgctc
     1621 ctcgcacagt ttcaatcgtt gaagttatgc catagtcttg taacttttta cgaacacgag
     1681 tcatgtttac gtttaaagta ttttcatcaa caaaagcttg gtcatcccat agttcttcga
     1741 gcaattgttc acgagtgacc actttggggg actggcttaa caaaagctcc aatattgtac
     1801 attctcgctt ttgaagagga atttcaagtg acacaaaatg cagttccatg cgctctaaat
     1861 ataaacatag tttgcctagt ttgatggtac gttcttcttg tttcaatgca tactcaccgt
     1921 aagttcttcg taaatgactt cttattttgg ctaagacgat ttcataatga aaaggtttcg
     1981 taatgaaatc a
//
LOCUS       DASWBY010000624         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_287631, whole genome shotgun sequence.
ACCESSION   DASWBY010000624 DASWBY010000000
VERSION     DASWBY010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287631"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..359)
                     /locus_tag="VF870_11375"
     CDS             complement(114..359)
                     /locus_tag="VF870_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252835.1"
                     /translation="MTKEYKPIACGLYDELELRALRKKPVKLTITNNKNQTEIFECII
                     VDLFSKDKTEFLKTGDGKIIRLDDVVELDDIIFNKNC"
     gene            complement(503..1276)
                     /locus_tag="VF870_11380"
     CDS             complement(503..1276)
                     /locus_tag="VF870_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252836.1"
                     /translation="MRYLSTLSIFLLGGFFLLAPKSFSQEMKDQMFLIHEEVVNINMW
                     DKYESTSKEWVQMMNGAGLDLPYIQASQRDDGHYYYIIPISNYAEIDKMQGIFNSAIE
                     KIGKDKWRKFMVENNSTIQGSKDFVATRSAKYSYMPADPRTKQEDVKFIHWMFFTYKM
                     ENRKEVMDILSEWKKLYEDKKIKSGYQIWLIELGLDNNMIALTEGYKDGADFYTSQQA
                     DNALMEKEASALWAKMSSFITSIDNKYGNPRPDLGYVKK"
     gene            complement(1426..1779)
                     /locus_tag="VF870_11385"
     CDS             complement(1426..1779)
                     /locus_tag="VF870_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9252837.1"
                     /translation="MIDKKQMTKDYKQQKQPAGVYAVHNKVDNKMYIGTSKNLPAVIR
                     RFEFTLQMESFPYQDLIDDYKNLGKNNFEIKIIDELELKNETEKEIDVELIALEELWI
                     EKLKDEGVSFYNKKN"
     gene            complement(1776..2426)
                     /locus_tag="VF870_11390"
     CDS             complement(1776..2426)
                     /locus_tag="VF870_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252838.1"
                     /translation="MFIGHFGAGFAGKKFEKSASLGTYFMAAQWIDLIWPILLLLGIE
                     KAEIKPGVTIVTPLDFTYYPFTHSLFGVIVWAVLFGMVYFVIKKNSRTAIILALLVLS
                     HWCLDLFVHIPDLPIFPGASIKVGFGLWNSFAATIILEGLIFIVGVYLYYKSTKPKNK
                     IGVFSLIGLIVFLVAMYISNLVGPTPPSVEMVGIVGNAQWLIVLSGYWIDKNREAI"
BASE COUNT          783 a          447 c          368 g          877 t
ORIGIN      
        1 tttgatcatt ttctgcaagc gaaaacccca aaactttttt ccagacttct gcatctgccc
       61 acttcatatg gtttaataag gtttctattg atcccgattt catattaatt ctttcagcag
      121 tttttattaa aaattatgtc atctagttct accacatcat ccagacgaat gatttttcca
      181 tcgcccgttt taagaaattc tgttttatcc tttgaaaaca aatcaacaat tatacattca
      241 aatatttcag tctgattttt attatttgta attgtaagct taacgggttt tttccttaaa
      301 gcgcggagtt caagttcatc ataaagtccg caagctatag gtttatattc tttggtcata
      361 gtgatgtctt taaatatagg tccaattgca aggtcggctt atttgttttt gaaaaaggtt
      421 tttaattatc aaatgaaata ttattaaaca aaaaagccga tacaaatatt atttatatcg
      481 gctcatgaat aaaatatttt aattactttt taacatatcc aagatctggt cttgggttgc
      541 catatttatt atcaatggaa gtaataaatg aagacatttt tgcccacaga gcacttgcct
      601 ctttttccat taaagcatta tctgcttgtt gggatgtata aaaatcagcg ccgtctttat
      661 aaccttctgt taaggcgatc atgttattgt ctaatcctaa ttcaatgagc caaatttgat
      721 aacctgattt tatttttttg tcttcgtaaa gttttttcca ttctgaaagt atgtccataa
      781 cttcttttcg gttttccatt ttataagtga agaacatcca atgaatgaac ttaacatctt
      841 cttgttttgt tcttggatca gctggcatgt aagaatattt ggctgatctt gtagcaacaa
      901 aatctttact gccttggata gtagaattat tttctaccat gaactttctc cacttatcct
      961 ttccgatctt ttctattgcc gaattaaaga ttccctgcat tttatcaatc tcggcataat
     1021 ttgaaattgg gatgatataa taataatgac catcatctct ttgagaagcc tggatataag
     1081 gaaggtcaag tccagcccca ttcatcattt gaacccattc tttggacgtt gattcatatt
     1141 tatcccacat attaatattt accacttctt catggatgag aaacatttga tctttcatct
     1201 cctgagagaa agattttggt gcgagcagaa aaaatccgcc gagtaaaaag atactaagtg
     1261 tactgagata gcgcataatg tgcctcctta ggttggttga taattatttg ttgaaacact
     1321 taatcggaaa agtgttttag tatacacaag aaactatgaa atgaattttt aagtagcaag
     1381 aaaaatatta ctatgcagtg ctataagttg attgtgttca gctatttagt ttttcttatt
     1441 ataaaaactt acgccttcat cttttagttt ttctatccat aattcttcaa gcgcgattaa
     1501 ttcaacatca atttcttttt cggtttcgtt ttttaattca agttcatcaa taatttttat
     1561 ctcaaaatta tttttaccaa gatttttgta atcatcaatc aaatcctggt aaggaaaact
     1621 ttccatctgc aaagtaaact caaatcttct aattaccgca ggcaggtttt tacttgtgcc
     1681 gatatacatt ttattatcaa ctttgttatg tacagcataa acgcccgcgg gttgtttctg
     1741 ttgcttgtaa tcttttgtca tttgtttttt gtctatcata ttgcctctct gtttttatca
     1801 atccagtaac ccgatagtac aattagccat tgtgcattgc ctacaatacc aaccatctca
     1861 actgatggag gagtaggacc aacaagattt gaaatataca ttgcaaccag gaacacaatt
     1921 aatccgatca gcgaaaaaac cccaatttta ttttttggtt tagttgattt atagtataag
     1981 taaacaccca caataaaaat taatccttct aaaattattg ttgcggcaaa actgttccat
     2041 aaaccaaaac cgactttaat gcttgccccg ggaaatatcg gcagatcagg aatgtgaaca
     2101 aacaaatcta aacaccaatg gcttaaaaca agcagcgcaa gaatgattgc agttctggaa
     2161 ttttttttga taacaaagta aaccatacca aacagcactg cccaaacgat tactccaaat
     2221 aaactgtgcg taaaagggta gtaggtaaaa tctaaagggg taacaattgt aacgccaggt
     2281 tttatttctg ctttttcaat tcccaacaga agaagtatcg gccagataag atctatccat
     2341 tgtgcagcca taaagtatgt gccgagtgat gcggactttt caaatttttt accagcaaag
     2401 ccggcaccaa aatgaccaat aaacatattt acctcattaa ttaataaaac agttcagtta
     2461 atgataaaaa tattc
//
LOCUS       DASWBY010000625         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_439772, whole genome shotgun sequence.
ACCESSION   DASWBY010000625 DASWBY010000000
VERSION     DASWBY010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439772"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VF870_11395"
     CDS             <1..572
                     /locus_tag="VF870_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX9252839.1"
                     /translation="TVEVEGEFAYGYLKAENGVHRLVRISPFDSNKRRHTSFASVFVI
                     PEVDDTIEIEINPADLRVDTYRSGGKGGQNVNKVETAVRFTHIPTGIVAACQSERSQA
                     QNRVNAMKLLKSKLYQLELEKQNAALDEVEKSKMKIEWGSQIRSYVFHPYNMVKDHRT
                     DEETSDTQGVMDGDINRFIKAFLLKFSQN"
     gene            651..3248
                     /locus_tag="VF870_11400"
     CDS             651..3248
                     /locus_tag="VF870_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9252840.1"
                     /translation="MRLLERDQFISDLSALFERIKNENGFIVSISGEAGIGKTSLVEH
                     FTNSYKDQAQILWGSCDDLFTPRPLAPLYDIADQLNSKITEQLDSGSPRPSIFSNLLK
                     EIHNRVPKIIVIEDMHWADESTLDFLKFLGRRINKTQALLIITYRDDEIKSDHPLKLA
                     LSNLPSNYLKRIKLSPLSENTVNTLALKYGRKDSELYTKTGGNPLLVTEVLANEQQET
                     STTIKELFTSKLKRLTLDGRSIVEMFSVIPGKVEKWLAQKLINDLSKVDEILDCGILK
                     SEGDSYLFRHELSRIAIEDSLSESKRINLNSKILDVLVHQQNIDHYLSRIIHHASKAF
                     NKDAIIKYAPSAGKQASKLGAHLQAVKIYQAAIQCSDKLSVEQKLDLLESISYECFIT
                     GQVYEAIQAAESAIKILKEYPAQEREGEIYRKLSRILWYDCQDQKGEEYLNKAIQIFE
                     TLPPSRKLAMAYSNKSQTYAIREENNFAIEWGNKALELARKFNDKEVEAHALNNIGCC
                     KMLIEDHSGENDLLKSLDISVENDFYEHATRAYVNLGSIHLQQRNLIKAENCFAKGLE
                     YGNEKDIYVFSLCMAGHYAKTKLLFGMWDESIDLANYVLKQKNAPPGNTVMPLNVLAV
                     IRTRRNDPGALKLINEAMEMAINMGEMEKIVSVASAKAEYFWLQNKLDDFSYELISIY
                     SKVIKQDNPWAIGEIAYWICKADRLKEIPKIIAKPYLLQIQGNWKEAARVWENLRCPY
                     EQALALSEGDEEAMKKSIEMLEKLGASATVQLIKQKMRESGIRSIPKGPRKTTRDNFA
                     GLTSRQLEVLKLLAHGMSNLEIANNLFISAKTVDHHISAIFSKLNIHSRIEAASFLQS
                     NFNNQLAQK"
     gene            3348..3620
                     /locus_tag="VF870_11405"
     CDS             3348..3620
                     /locus_tag="VF870_11405"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX9252841.1"
                     /translation="MPRYVIERIFKNGLLIPRSPEGQRICLGVVDNNLEDNVTWVHSY
                     VSDDKTKSFCIYDAPTPEALRKAASRNNLPIEKITEVNVLDPYFYI"
     gene            3649..4026
                     /locus_tag="VF870_11410"
     CDS             3649..4026
                     /locus_tag="VF870_11410"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX9252842.1"
                     /translation="MKSILFLLFSFFVACLFTTDNYAQTNNKNDGKVVMHRYLVERSF
                     PDGLYIPVNAEGSEICLTVVSTNAEDNVSWVHSYVTMDKKKTFCIYDAPSPEAIRKSA
                     KKNGFPPVDNIIEVSVLDPYFYK"
     gene            4055..4591
                     /locus_tag="VF870_11415"
     CDS             4055..4591
                     /locus_tag="VF870_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252843.1"
                     /translation="MKSLSVFSFFPLLFAILIFAGCNQQENSPVEGSIGGNDDSFLAK
                     QYNLPKDVMQELQEAKIATARYNNLENAIADGYEDINVVMPNMGHHYMKANYVDGIFE
                     IDKPEILVYSPHPVTGNMRLVAVEYAIPNTEPEPAGFTGSYDVWANNAGFGLWLLHAW
                     VWEYNPDGVFNPTNPLVP"
BASE COUNT         1667 a          733 c          885 g         1424 t
ORIGIN      
        1 caacagtaga agtagaaggc gagtttgcat acggttattt gaaagctgaa aatggcgtac
       61 atcgtttagt aaggatttct ccatttgatt ctaataaaag acgtcacact tctttcgctt
      121 ccgtgtttgt aatcccggaa gtagatgata caattgagat tgagataaat cctgcagatc
      181 taagagttga tacttatcgg tctggtggca aaggcggaca gaatgttaat aaagtggaaa
      241 cagcagttag atttacgcac attccaactg gaattgttgc agcatgccaa agtgaaagat
      301 cgcaggcaca gaatcgtgtt aatgcgatga agttattaaa atcaaaactt taccaactag
      361 aattggaaaa acaaaatgct gcattagacg aagttgagaa aagtaagatg aaaattgagt
      421 ggggtagtca aatccgttcc tatgtttttc atccatacaa tatggtaaaa gatcatagaa
      481 cggatgaaga aacttctgat actcagggtg tgatggatgg agatattaat agatttataa
      541 aggctttttt attgaagttt agccagaatt aaatttttga aaaacgactt gcaattgaat
      601 atttaaatag ttaattatgt acagttatta actatttagc acaggcaaat atgagattgc
      661 tggaacgtga tcaatttatt tctgatctta gcgctttatt tgaaagaata aaaaacgaaa
      721 atggattcat agtttcaatt tcaggtgaag caggaattgg taaaacatct ctcgttgaac
      781 attttacaaa ttcttataaa gaccaggcac agattttatg gggttcgtgt gatgaccttt
      841 ttacacccag accacttgca cctttgtacg atattgctga tcagctcaat agcaaaatca
      901 cggaacaatt agattctgga agcccccgtc catcaatatt ttcaaattta ttaaaagaga
      961 tacacaatag agttcccaaa attattgtta ttgaagatat gcactgggct gatgaatcaa
     1021 cgcttgattt tctgaagttt ttaggaagaa gaattaataa aactcaagca ttattaatta
     1081 taacatatcg ggatgatgag ataaaatctg atcatccttt aaaattagct ttaagcaatc
     1141 tcccttctaa ttatctaaaa agaattaagc tttctccact ttcagaaaat acagtaaata
     1201 ctcttgcatt aaagtatgga agaaaagatt ctgaacttta cacaaagaca ggtggcaatc
     1261 cgcttcttgt aactgaagta ttagctaatg aacaacaaga aacatctacg acgattaaag
     1321 aactttttac ttcaaaactg aagcgattaa cactcgatgg aagatcaatt gtggaaatgt
     1381 tttctgttat tccaggcaaa gtagaaaaat ggcttgctca aaaattaatt aacgatttaa
     1441 gcaaagtaga tgaaatttta gattgtggaa tattaaagag tgaaggtgac tcatacttat
     1501 ttagacatga attatcaaga attgctattg aagattcact ctcggaatca aagagaatta
     1561 atctcaactc aaaaatactt gatgtattag ttcaccagca aaatattgat cactatttat
     1621 caagaataat tcaccacgct tcaaaagctt ttaataagga tgcaataatt aagtatgctc
     1681 cttctgcagg caaacaagca tcaaaacttg gtgctcatct acaagctgta aaaatttatc
     1741 aggcagctat tcaatgttct gacaaacttt cagtagaaca aaaattagat cttctggaaa
     1801 gcatttcata tgaatgtttt ataaccggtc aggtttatga agcaatacaa gcagccgaaa
     1861 gcgccattaa aattctaaag gaatatccgg ctcaagaacg agaaggagag atttatagaa
     1921 aattatcaag aattctttgg tatgattgtc aggatcagaa aggcgaggaa tatttaaata
     1981 aagcaatcca aatttttgaa acgctgccgc caagtagaaa acttgccatg gcctacagca
     2041 ataaatcgca gacttatgca ataagggaag aaaacaattt tgcaatcgaa tgggggaata
     2101 aggcgcttga gttagcaaga aaatttaatg ataaggaagt ggaagctcat gcgctcaata
     2161 atattggatg ttgtaaaatg ctaatcgaag atcattctgg tgaaaacgat ttattaaaaa
     2221 gtttagacat ctcagtcgag aatgattttt acgaacacgc aacgcgggca tatgtaaacc
     2281 ttggctcgat acatttacaa caaagaaatc taattaaagc agaaaactgt tttgcaaagg
     2341 gtttagaata tgggaatgaa aaagacattt acgtctttag tttgtgtatg gcgggccact
     2401 atgctaagac taaattactg tttggtatgt gggatgaaag catagatctt gctaattatg
     2461 tcttgaaaca aaaaaatgct ccgcctggta atactgtgat gccattaaat gttcttgctg
     2521 taatcaggac acgaagaaat gatccaggag ctttgaaact cattaatgaa gctatggaga
     2581 tggctattaa tatgggtgaa atggaaaaga ttgtaagtgt agcctcagct aaagcagaat
     2641 atttttggtt gcaaaataaa ttggatgact ttagttatga actaatttca atttattcta
     2701 aagttattaa acaggataat ccctgggcaa ttggtgaaat tgcctattgg atctgtaaag
     2761 cggatcgatt gaaggaaata ccaaaaatta ttgccaaacc gtacttactt caaatacaag
     2821 ggaattggaa agaggctgcc cgagtgtggg aaaatttgcg atgtccttat gaacaggctt
     2881 tggctctgtc agaaggtgat gaagaggcaa tgaaaaaatc tattgaaatg cttgaaaaat
     2941 taggagcatc agccacagta caattgataa aacaaaagat gagagaatca ggaattcgaa
     3001 gcatcccaaa aggtcctcgt aaaacaacca gagataactt tgccggatta acatcaaggc
     3061 aactggaagt attaaaatta ttagctcacg gaatgagcaa tttagaaatt gcaaataatt
     3121 tgtttatttc agctaagaca gtcgatcatc atatatctgc tattttttcc aagctcaata
     3181 ttcattctcg gatagaagcc gcatcatttt tacaatctaa tttcaataat caattagccc
     3241 aaaaataggg aacacctaac gataaaatag ggaatcctcc cgattccttt cttttcattt
     3301 cacaacatat ttacttcaga tattttattt aataaaacgg agataatatg cctcgttatg
     3361 ttatcgaaag aatatttaaa aatggactat taattcctag gtcaccggaa ggtcagagaa
     3421 tatgtctcgg agttgtagat aacaatttag aagacaatgt aacctgggtc cattcttacg
     3481 taagtgatga caaaacaaaa tcattctgta tttacgatgc tccaactcct gaggctttac
     3541 gcaaagctgc cagcaggaat aatcttccaa tagaaaaaat aactgaagtg aatgttcttg
     3601 acccttattt ctatatataa atcactaaaa aaaccttagg aggttaaaat gaaaagtatc
     3661 ttatttctcc ttttttcttt ttttgttgct tgtttattta caacagacaa ctatgcacag
     3721 actaataata agaatgatgg caaagtagtt atgcatcgtt accttgttga gcgcagtttt
     3781 cccgatgggc tttatattcc tgtaaatgca gaaggaagtg aaatttgtct tactgttgtt
     3841 tcaacaaatg ctgaagataa cgtttcatgg gtacattcat acgtaactat ggataagaaa
     3901 aaaacatttt gcatctatga tgctccatca cccgaggcta taagaaagtc agctaaaaag
     3961 aatggctttc ctccagtgga taatataatt gaagtaagcg ttctggaccc ttacttctat
     4021 aaataataat caaccaacta atctaggagg ctctatgaaa tcgttaagtg tattttcctt
     4081 ttttccattg ctgtttgcca ttctcatttt tgcaggatgt aatcaacagg aaaactcacc
     4141 tgttgaagga agtattggtg gaaatgatga ttcatttctt gcaaaacagt ataatcttcc
     4201 aaaggatgtt atgcaggaac ttcaggaggc aaaaattgca actgcaaggt ataacaatct
     4261 tgaaaatgca attgctgatg gatatgagga tataaatgtt gtaatgccta atatgggtca
     4321 tcattacatg aaagcaaatt atgttgatgg tattttcgaa attgataaac cggaaatttt
     4381 agtttactct ccgcatcctg ttaccggtaa catgaggctg gttgctgtag aatatgcaat
     4441 tccaaatact gaacccgaac cagcaggttt tactggaagc tatgatgtat gggctaataa
     4501 tgcaggtttt ggtttatggc tgttgcatgc gtgggtttgg gaatataatc ctgatggtgt
     4561 ttttaatcct accaatccgc ttgtccctta aaaaatataa tttaatataa tggttattaa
     4621 ggcagtttat cataaactgc ctttttaatt cttcttaata aatagaataa catcacataa
     4681 tgttaatttt tggaagaaca taaagaact
//
LOCUS       DASWBY010000626         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_361426, whole genome shotgun sequence.
ACCESSION   DASWBY010000626 DASWBY010000000
VERSION     DASWBY010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361426"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VF870_11420"
     CDS             complement(<1..261)
                     /locus_tag="VF870_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9252844.1"
                     /translation="MKKHLIQIILLVLFIFNPFANADVNSEMQKANELYKNNQYQLAI
                     DEYNKLISQGYEGASLYYNLGNAHYRLGKVGYAILYYEKALKL"
     gene            complement(258..>1939)
                     /locus_tag="VF870_11425"
     CDS             complement(258..>1939)
                     /locus_tag="VF870_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEX9252845.1"
                     /translation="FTFEGKNLNALKNFNPPSLKDFRVLSGPNQSTSMQIINGVLSSS
                     LTLSYILLPNTTGAYTIGSASIVYDGKPYTTDPIKISVVKGTPKPKDDSGSQISNEEI
                     AKNLFIRATIDKNKVYLGEQVTITYKLYTRLNIASQMSVDKLPQYQGFWAEELETARN
                     ISFSREVIDGQQYNVGLLKRAALFPNQAGKLEVTPFELTIPIQVQKKKNPNNVWDDFF
                     GDPFGRSEIVEYKAKSNTLKVDVMPLPENNKPASFKGAVGKFDFSAVIDKQKTKTNEP
                     INLKLDISGTGNIKLLDLPPFELPNGFEKYDPKVDEQINRSGKISGSKKAEYLLIPRV
                     AGSREIPPIEFSFFDPEKKSYQTIRSKAFNVTIEQGKSSGNDFANQQNVEQLESDIRF
                     IKTKFNDIDKRDEIVLLQPGFWIASIIPFLASLVLIGWKRKQDKLSGNQQLLRYSKAE
                     KVARKRFKSAKKLLDQNNIELFYSEISQALFGYLEDKFHIAKSEFTLDRAANELLKRN
                     IKPELVEKMKTNAQKCEFIRFAPGTNPSQAMNEMYKSFSDVVIDIERSIAK"
BASE COUNT          580 a          354 c          314 g          691 t
ORIGIN      
        1 taactttaag gctttttcat aatacagaat agcgtatcca accttgccca aacggtaatg
       61 tgcattccca agattgtagt aaagtgaggc cccttcataa ccttgactaa tgagcttatt
      121 atattcatca attgcgagct gatattgatt gtttttgtac aattcattcg ccttttgcat
      181 ttcagagttt acatctgcat ttgcaaatgg attaaaaata aaaagtacta aaagaattat
      241 ttgaattaaa tgctttttca ttttgcaatt gatctttcaa tatctataac aacatccgaa
      301 aaagatttat acatttcatt catagcctgt gaaggattgg tccctggtgc aaatctaata
      361 aactcacact tctgtgcatt ggttttcatt ttttctacaa gttcaggttt gatatttctt
      421 tttaacaatt cattggctgc tctgtcaaga gtaaactcag atttagcgat atgaaattta
      481 tcttcaagat atccaaataa tgcctgtgaa atctcagaat aaaaaagctc aatattattt
      541 tgatcaagaa gtttttttgc tgacttaaat cttttgcgcg caactttttc tgctttggag
      601 taacgcagta attgctgatt gcctgaaagt ttatcctgtt ttcttttcca tccgattaaa
      661 actaacgaag ctaaaaatgg gattattgaa gcaatccaga atcctggttg caataaaaca
      721 atttcgtctc gcttatcaat atcattaaac tttgttttga taaaacgaat atcgctttca
      781 agctgttcca cattctgctg attagcaaaa tcgtttccac tgcttttccc ctgctcgatc
      841 gttacattaa aagcttttga gcgaattgtt tgataagatt ttttttcagg atcaaaaaaa
      901 gagaactcaa ttggcggaat ctcacgagag cccgctactc tgggaatcaa aaggtactcg
      961 gctttttttg aaccgcttat ttttccgctt ctattaattt gctcatcaac ttttggatca
     1021 tacttctcaa aaccgtttgg taattcaaag ggcggcagat ccaacagttt aatatttcct
     1081 gtcccggaaa tatctaactt taaattaatt ggctcgttag tttttgtttt ttgtttatca
     1141 ataaccgcag aaaaatcaaa cttacccacg gcacctttaa aggatgctgg tttattattt
     1201 tccggaagcg gcatcacatc aacttttaga gtatttgatt ttgctttata ctcaacaatt
     1261 tctgagcgtc caaatggatc accaaaaaaa tcatcccaaa cattatttgg attttttttc
     1321 ttctgaacct gaatcggaat tgtaagttca aatggagtaa cttctaattt gcctgcctga
     1381 ttcggaaaca gtgctgcgcg ctttaataat ccaacattgt actgctgacc atcaattact
     1441 tccctgctaa aggaaatatt acgtgcggtt tcaagttcct ctgcccaaaa tccctggtac
     1501 tgcggtagtt tatccacgga catctgggat gcaatgttta atcgggtata gagcttatat
     1561 gtaatggtaa cctgttcacc taaataaact ttattcttat ctattgttgc acgtataaat
     1621 aaattttttg caatctcttc atttgaaatc tggctgcctg aatcatcttt tggttttggg
     1681 gttcctttaa caacagaaat ttttattgga tctgttgtat aaggttttcc atcatatact
     1741 atagatgcac tgccaattgt atatgctccg gttgtgttgg gcaataaaat ataagataaa
     1801 gttaatgatg atgataacac accattaata atctgcatac tggttgattg attaggaccg
     1861 gaaagaactc taaaatcttt taaggagggt ggattgaaat tttttaacgc attaagattt
     1921 ttaccttcaa aagtaaacc
//
LOCUS       DASWBY010000627         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_137929, whole genome shotgun sequence.
ACCESSION   DASWBY010000627 DASWBY010000000
VERSION     DASWBY010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137929"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /gene="pgk"
                     /locus_tag="VF870_11430"
     CDS             complement(<1..550)
                     /gene="pgk"
                     /locus_tag="VF870_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9252846.1"
                     /translation="MNKLSIDKVELKGKRVLVRVDFNVPLDENLNITDDIRITESLPT
                     IKKIIADGGKAILMSHLGRPKGGPNPKYSLKPTAKKLSELLGNEVKLAPDCIGSETKA
                     MVDAMKDGDVLILENVRFHAEEEKNDPAFAKQLADLGDVYINDAFGSAHRAHASTEGV
                     TRYINICAAGYLMQKELDYLGGA"
     gene            complement(625..>1561)
                     /gene="gap"
                     /locus_tag="VF870_11435"
     CDS             complement(625..>1561)
                     /gene="gap"
                     /locus_tag="VF870_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX9252847.1"
                     /translation="DAKYFAYQLKYDSVHGIFKGEITSKKSKADLEHDDVLVINGEET
                     LCVLATKDPSQLPWKELGVDYVIESTGLFTDSEKAKGHIVAGAKKVIISAPGKGDVKT
                     VVMGVNDNELKPEHTIISNASCTTNCLAPVVHVLLKEGIGVEVGIMTTIHSYTATQKT
                     VDGPSKKDWRGGRAAAINIIPSSTGAAKAVGEVLPSVKGKLTGMSFRVPTANVSVVDL
                     TFRAAKDTSIKEIDELLKKASATYLKGILGYTDEDLVSTDFNHDDRSSIYDSLATLQN
                     NFKDEKRFFKIVSWYDNEWGYSCRCVDLLSKLIKM"
BASE COUNT          456 a          315 c          243 g          547 t
ORIGIN      
        1 tcgctccgcc aagataatca agttctttct gcatcaaata tcccgcagca caaatattta
       61 tatacctggt tacaccttct gtagaggcat gagctctgtg tgcacttcca aacgcatcat
      121 ttatataaac atctccaaga tctgctaatt gttttgcaaa agccggatca tttttttctt
      181 cttcagcatg aaagcgaaca ttttcaagaa tcaaaacatc gccgtctttc attgcatcca
      241 ccatagcttt ggtttctgac ccaatgcaat ctggagctaa tttaacttca ttaccaagta
      301 attcgcttaa tttttttgca gtcggtttta aactatattt cggatttggt ccacctttag
      361 gtctgccgag atggctcatc aaaattgctt tgccgccatc agcaataatc tttttaatag
      421 ttggtaatga ttctgttatt ctgatgtcat cagtaatatt taaattttca tccagcggaa
      481 cattaaaatc aacgcgaaca agaacacgtt ttcctttaag ttctacttta tctattgata
      541 gcttattcat tttttcttcc ctgagtataa attttaatta atattaaaaa ggcgatccaa
      601 ttaagatcgc cttaggttga ataattacat ttttattaat ttggataaca gatcaacgca
      661 gcggcatgaa tatccccatt cgttatcata ccagctgaca attttaaaga atcgtttttc
      721 atctttaaag ttattctgta atgttgctaa agaatcataa attgaagaac ggtcatcgtg
      781 attaaaatct gtagaaacta gatcttcatc tgtgtaacca agtatgcctt tcaaatatgt
      841 agcagaggct ttcttgagta actcatcaat ttcttttatt gacgtatctt ttgctgcacg
      901 gaatgttaaa tcaacaactg aaacatttgc agtaggaaca cggaatgaca ttcctgttag
      961 ttttcctttt accgaaggaa gaacttcacc aactgctttt gctgcgcctg tgcttgatgg
     1021 aataatattt attgcagcag ctcttccgcc tctccaatct ttcttggaag ggccatcgac
     1081 tgttttttgt gttgcagtat aagagtgaat ggttgtcatt attccaactt caactcctat
     1141 tccctccttc aataaaacgt gaacaacagg tgccaaacag tttgttgtac atgaagcatt
     1201 agaaatgata gtatgttctg gttttaattc gttatcatta acacccataa caactgtttt
     1261 aacatctcct ttacccggag cagaaatgat aacctttttt gcacctgcaa caatgtgtcc
     1321 ttttgctttt tcagaatctg taaaaagtcc ggttgattca attacataat caactccaag
     1381 ttctttccac ggtaattgcg aaggatcttt tgtcgcaaga acacacaaag tttcttcgcc
     1441 atttattaca agaacatcat cgtgttcaag atcggcttta cttttctttg atgtaatttc
     1501 acctttgaaa attccgtgaa cagaatcata ctttaattgg taagcaaaat attttgcgtc
     1561 a
//
LOCUS       DASWBY010000628         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_175849, whole genome shotgun sequence.
ACCESSION   DASWBY010000628 DASWBY010000000
VERSION     DASWBY010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175849"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VF870_11440"
     CDS             complement(<1..311)
                     /locus_tag="VF870_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX9252848.1"
                     /translation="MEIIYLIIGLVIGFVIAFFFFKSKKRVPIEEANKLNDQINTFKV
                     DAGKLAERIKLFEEDKLSLQSELKIEREKSERLTSENSSLKSDYANLQEKLSEQKSEV
                     E"
     gene            complement(420..1943)
                     /locus_tag="VF870_11445"
     CDS             complement(420..1943)
                     /locus_tag="VF870_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEX9252849.1"
                     /translation="MSLILDGSNLTVEKLVKIARHKEKVEISNEALQRIKTCRAMLEE
                     KIQAHEIMYGVNTGIGEFSEVVLNDEQVKDFQKYLIYNHSAGIGEPMPIEHVRGAITG
                     RINVHAHGNSGCRPEITLTLIEMLNKDCIPVVCEKGSVGACGDLAPMSQIALLLLGEG
                     EAFYNGERLPGKVAFEKAGIKVPGLQARDGLATINGSNFITAISALQLFDINRWLKQA
                     EIAAAMSLEALQANMKPYDVRLHKIRGFAGAVRSSQAIKKCIEGSDLLTGKIKQKVQD
                     AYSMRSTPQVIGAAHDAVAYAKSQIEIELNGVGDNPIFIPEDKITLTGANFQGTPVSL
                     PMDMISAAVTMVSVLSERRMNRLNNPALSVGLPAFLTKGAGMFSGMMLSQYTADTLIV
                     EQRILSAPASVQSIPAAADQEDFVSMGMNTVLKNQQILDNAYGILGIEFMAAAQALDF
                     REHVPGKGVAKAKEVIRKHVEFLDIDRPLYADHNKMKTLVKSCEILEEVEKTIGNLE"
BASE COUNT          609 a          414 c          344 g          718 t
ORIGIN      
        1 ttttctactt cggatttttg ttcagataat ttttcttgaa gatttgcgta atcagattta
       61 agtgatgaat tttcagatgt taatctttcg gatttttctc tttcgatttt taattctgat
      121 tgaagtgaaa gcttatcttc ctcaaacagt tttattcttt cagcaagttt gcccgcatca
      181 actttaaatg tgttgatttg atcatttagt ttatttgctt cctctatggg cacccttttc
      241 ttgcttttga aaaagaaaaa agcaattaca aatccgatta ctaaaccaat tattagataa
      301 attatttcca ttttatatcc ttttaatctc aatgggacac ggatctaact gatcaaacag
      361 atttgaactg atttaatctg tgtatatcag tttaatctgt tgaattcgcg ttccattctt
      421 tattctaagt ttccgatagt tttttcaact tcctcaagaa tttcacagga ttttacaagt
      481 gttttcatct tgttatgatc agcataaagc ggacgatcaa tatcaagaaa ctcaacatgt
      541 ttacgaatta cttcttttgc ttttgcaact ccttttccag gaacatgttc acggaagtca
      601 agagcctgag cagcagccat aaattctatt cccaatatac cataagcatt atccaaaatc
      661 tgttgattct taagaacagt attcattccc attgaaacaa aatcctcctg atccgcagca
      721 gcaggaattg attgaactga agcaggtgca gataaaattc tttgttcaac aattaatgta
      781 tctgctgtat attggcttaa catcataccg gaaaacattc ctgccccttt tgtaagaaat
      841 gcaggcaagc caacgcttaa agcaggatta tttaatcgat tcattcttct ttcggataaa
      901 acactaacca tagtaaccgc tgcactaatc atatccatcg gaagtgaaac tggcgttccc
      961 tgaaaatttg caccagtcaa agtaatttta tcttcaggaa taaaaattgg attgtcccca
     1021 actccgttta gctcaatttc aatttgtgat ttagcataag caacagcatc atgagctgca
     1081 ccaataacct ggggagttga gcgcattgaa tatgcgtcct gcactttttg ttttattttt
     1141 cccgttagaa gatcgcttcc ttcaatacat ttttttattg cttgagaact tctaacagcg
     1201 ccggcaaaac cacgaatttt gtgtagacga acatcataag gtttcatatt cgcctgaagt
     1261 gcttcgagtg acattgcagc agcaatttct gcctgcttta gccatctatt gatatcaaat
     1321 aattgcaaag cgctaattgc cgtaataaaa ttagaaccgt taatcgttgc aagtccatcg
     1381 cgtgcttgta aacctggaac tttaattccg gctttttcaa aggcaacttt tccgggtaat
     1441 ctctcaccat tataaaatgc ttctccttct ccaagcaaca gcaaagcgat ttgagacatt
     1501 ggtgctaaat ctccgcaagc accaacagaa cctttttcgc acacaactgg aatacaatct
     1561 ttgtttaaca tttcaattag tgttaatgta atttccggtc tgcagccgga gttaccgtga
     1621 gcgtgcacat ttattctacc ggtaattgca ccacgcacgt gctcaatcgg cattggttcg
     1681 ccaattccgg ctgagtgatt atagataaga tacttctgaa aatcttttac ctgttcatca
     1741 tttaaaacaa cttcggaaaa ttctccgatg ccggtattta ctccgtacat aatttcgtgt
     1801 gcctggattt tttcttcaag cattgcgcgg caagttttta tcctctgaag agcttcgtta
     1861 cttatttcta ctttttcttt gtggcgggca attttaacta atttttctac tgtgagattt
     1921 gatccatcaa gaatgagtga cattattcct ccgagtgatg aaaattttaa taagcaaatt
     1981 tagttataag attggaaaaa gaagagttca agtttaggtt taaggaaatc tttgatagtc
     2041 attacaagga gtcctgaacg aagcaaattc atattttaca agatg
//
LOCUS       DASWBY010000629         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_662449, whole genome shotgun sequence.
ACCESSION   DASWBY010000629 DASWBY010000000
VERSION     DASWBY010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662449"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..>1669)
                     /locus_tag="VF870_11450"
     CDS             complement(110..>1669)
                     /locus_tag="VF870_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9252850.1"
                     /translation="ADVTMVDTFFTFKDVDLISNVPPTIASTTPAQNDSLYPGVDNVV
                     INFTRQMNKTSVETNLTITPTVAYTTAWSNNDKTLTINTNNFAFSSQYDITIGGSAEG
                     KYGHPFDGDGNGVGGDAFTLTVKTKVADVTAPTVADVYPSANATNVEYKPVINVSFDE
                     LLKTSTISSRFKIVRNSTQTNAAGILRHYVVNGRSVLNFFITTPLFENESYTIKVLAG
                     MEDIFGNPTPVDYNYEFTTGNSNYFAQSIIDNYEAGISNWWQPGNSGNTVGTNPLTTK
                     IYSNTTVLNSNTGSTKSMQLDYEWNTNASDWLIREYYSPSTPTFEPNTLLQVFMFGDG
                     SNNKFRFAVRETAPGTYEVSPWYDINWIGWKIVTWDLTQGQTGTWLGNGVFEPPLSFD
                     SFQLTYEPGNQNTGTIYFDDLRTAAFNPTDVEEETGTITPTEYTLKQNYPNPFNPSTE
                     IKFSVPQSSNVKLIVTDILGKEVATLVNDNLNAGNYNVNFDAAALSSGVYFYTLITDN
                     FKQSKKMILMK"
BASE COUNT          499 a          306 c          279 g          585 t
ORIGIN      
        1 ttaccgaatt ttctgtagtt catataaatt ttctattacg aataaaaaag tcacccttcg
       61 acttcgctca gggtgacaaa tatttattaa ctcattactg acacaattat tacttcataa
      121 gtatcatttt tttactttgc ttaaagttat cagttatcaa agtgtaaaag taaactccac
      181 tcgataatgc cgctgcatca aaattaacat tgtaattacc ggcatttaaa ttatcattta
      241 ccaaagttgc aacttctttc ccaagaatat ctgttactat cagtttaacg ttagatgatt
      301 gcggaacact gaatttaatt tctgtacttg gattaaaagg attcggataa ttttgtttaa
      361 gtgtgtattc ggtaggtgta attgtgcccg tttcttcttc aacatcagtt ggattaaaag
      421 ctgcagttct taaatcatca aaatagatcg tacccgtatt ttgatttcca ggttcatatg
      481 tcaattggaa actatcaaaa cttaatggtg gttcaaacac accgttccct aaccaggttc
      541 ccgtttgtcc ctgagttaaa tcccaggtga caattttcca accaatccaa ttaatatcat
      601 accaaggact tacttcataa gtacctggcg cagtttctct tactgcaaat ctaaatttat
      661 tatttgagcc atctccaaac ataaatacct gtaaaagagt atttggttca aatgttggag
      721 ttgaaggtga atagtattct cttatcagcc aatctgaggc attagtgttc cattcataat
      781 ccagctgcat agattttgta cttccggtgt ttgaatttag taccgtggtg ttagaataaa
      841 tttttgttgt aagcggattt gttccaacag tgtttccgga gttaccaggc tgccaccaat
      901 ttgaaatgcc ggcttcataa ttatcgatta tgctttgcgc gaaataattt gaattaccgg
      961 ttgtaaattc ataattataa tctaccggag tcgggttacc gaaaatatct tccatccccg
     1021 ctaatacttt tatggtgtag gattcatttt caaataacgg agttgtaata aaaaagttta
     1081 atacacttct tccgtttaca acataatgcc ttaaaattcc agccgcgtta gtttgagttg
     1141 agtttctaac aatcttaaat ctgcttgata tggttgatgt tttaaggagt tcatcaaacg
     1201 aaacattaat gactggttta tattcaacat ttgtagcatt tgctgaagga taaacatctg
     1261 ccacagtcgg tgcagttaca tcagctactt ttgtttttac agtcagggta aaagcatctc
     1321 cacccacacc attaccatct ccatcaaatg gatgtccata tttcccttca gcacttcctc
     1381 cgattgtaat atcgtattgt gaactgaaag caaaattatt agtgttgatt gttaaagttt
     1441 tatcgttgtt gctccaggca gtagtataag ctactgttgg tgtaattgta agatttgttt
     1501 ctacagaagt tttattcatc tgtcgtgtaa aattaatgac aacattatca actccgggat
     1561 ataagctgtc attctgtgca ggagttgttg aagcgattgt cggcggaaca tttgaaatta
     1621 aatccacatc tttaaacgta aaaaatgtat ctaccattgt tacatcggc
//
LOCUS       DASWBY010000630         4183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_662479, whole genome shotgun sequence.
ACCESSION   DASWBY010000630 DASWBY010000000
VERSION     DASWBY010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662479"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VF870_11455"
     CDS             <1..866
                     /locus_tag="VF870_11455"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559815.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX9252851.1"
                     /translation="MQSWKLGVKANALYRDGSKLSQPLNTMSDEDIEAIMEDKEKNDI
                     VKVAEKIIHRYIAKRRRLPDRRSGYTQKAKINGQSVYIRTGEYDNGQLGEIFIDMHRE
                     GASFRSLINCFAISISLGLQHGVPLEEFTDAFVFTRFEPSGIVTGNDKIKMATSVIDY
                     IFRELAVTYLGRNDLSHVDADEIKNKASRGIIKKLSEPEFESEEIISERLVELDKDNP
                     EKYNYSPGSKVTATQERATHTVNSMTTKVKQAIENGYTGDICPECQSITMVRNGTCLK
                     CMTCGSTTGCS"
     gene            complement(951..1652)
                     /locus_tag="VF870_11460"
     CDS             complement(951..1652)
                     /locus_tag="VF870_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX9252852.1"
                     /translation="MTVLKKYFLYVFILISSLTLIACSGSSSSLRYNSNNENEKTKQT
                     TNTRYTSTETPSVDDTLAVIDDDISEFQDPSDLPEDEAKIDLTELMKNLDKKSTGSDG
                     ETFVSTKRELMLMEIIKYLNTPYKYGGNSLNGIDCSAFTQNVYRDSWMLELNRSAREQ
                     YQQGIVIDDRSELKFGDLVFFNTRKRVKPGHVGIYIGDNLFAHASSKNGVTVSSLDYD
                     YYNKRYMGARRIDEQ"
     gene            complement(1656..2510)
                     /locus_tag="VF870_11465"
     CDS             complement(1656..2510)
                     /locus_tag="VF870_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9252853.1"
                     /translation="MRIVKQFTNRDNQSLDRYLQEIGKVELLTPEQEIELTVRMKNGD
                     DSAMELLTKANLRFVVSVAKQYQNQGLSLGDLINEGNVGLIKAVRRFDETRGFKFISY
                     AVWWIRQAILQALAEQSRIVRLPLNQIGAINRIGKTYSNLKQEFEREPTSEEIAQALE
                     MDVDEISNTMKIYGKQVSVDAPFSLGDENSLLDVMENDEMPAPDTELISESLKAEIKE
                     VLSILPEREAEVIKLYFGLDEENPLTLEEIGDKFNLTRERVRQIKEKAIRRLRHNSRS
                     KILKSYLG"
     gene            complement(2661..3056)
                     /locus_tag="VF870_11470"
     CDS             complement(2661..3056)
                     /locus_tag="VF870_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252854.1"
                     /translation="MTLDKPLKFLLETDILVDYLTIHNSNKEPYLIKLMQRGICFTSV
                     LNASELFMMADTDLEKERVRDLLNALKVLGLHARYSLSIPACKNNFKSTRNALFYILA
                     EQNKLDIVTLNPDKYYGLRVNSKHPQTIE"
     gene            complement(3043..3225)
                     /locus_tag="VF870_11475"
     CDS             complement(3043..3225)
                     /locus_tag="VF870_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252855.1"
                     /translation="MNNSENVKNNPGDEQVFEKELLFRIIQIQDLPEEVVTKFLKAAG
                     SLSSTDFGKKYSDDLR"
     gene            complement(3218..>4183)
                     /locus_tag="VF870_11480"
     CDS             complement(3218..>4183)
                     /locus_tag="VF870_11480"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX9252856.1"
                     /translation="LASFGKMSELSKGKDSTTGHWELSGLYVDTDFDYFPNGFPKNIL
                     DKFLETTGCKGYLGNKAASGTEIIKVLGDEHLRTGFPIIYTSADSVFQIAAHQEVIPL
                     EKLYEICDKTRNKVLINPLKVGRVIARPFIGKSGSYERTVYRKDYSLDPPSETVLDLL
                     QKNIINTVAIGKINDLFNYRGISIQVKSKSNREGFEKLIKASNEYQQSLIFINLVDFD
                     VYFGHRNDFEGFAIALKEFDDFLPEFLENLDPTDRVIFTADHGNDPTTPSTDHSREYV
                     PVIYFGKNKSAKNLGIRNTFADVGKTAADYFKVKNDLKGTSFLNE"
BASE COUNT         1376 a          737 c          650 g         1420 t
ORIGIN      
        1 atatgcaaag ctggaaactt ggagttaaag caaatgcact ttatcgtgat ggatctaaat
       61 tatctcaacc tctgaacact atgagtgatg aagatattga agcgataatg gaagataaag
      121 agaagaacga tatcgtaaag gttgctgaaa aaattataca cagatacatt gctaaaagaa
      181 gaagattacc agatagaaga tcaggttata cgcagaaagc taaaattaac ggtcaatccg
      241 tttatatcag aaccggggaa tatgataacg gacagttagg tgaaattttt attgatatgc
      301 atcgtgaagg agcttctttc agaagtttga taaactgttt tgcaatttct atttccttag
      361 gcttgcagca tggagttcca ttagaagagt ttaccgatgc atttgtgttt acaagatttg
      421 aaccaagcgg aatagtaacc ggcaatgata aaattaaaat ggctacttct gtaattgatt
      481 atattttcag agaactcgct gtaacatatc ttggcaggaa tgatctttca cacgttgatg
      541 ctgatgaaat taaaaataaa gcatccagag gaattattaa aaaattaagc gagcctgaat
      601 tcgaaagtga agaaataatt agcgagcgtc ttgtagaact tgacaaagat aatcctgaaa
      661 aatataatta ttctccagga tcaaaagtaa ctgcaactca ggaacgagca acacatactg
      721 taaatagtat gactaccaaa gttaagcagg ccattgaaaa tgggtatact ggtgatattt
      781 gtccggaatg ccaaagtatt acaatggtta ggaacggaac ttgtttgaag tgcatgactt
      841 gtggttctac aacaggctgc agttaattcg aaacaaatct ttgataaaaa aaagctgttc
      901 aaataatgaa cagctttttt tatgagattg atttattctg attactaaaa ttactgttca
      961 tctattctgc gcgcacccat atatctttta ttataataat cataatcaag tgaagaaact
     1021 gttacgccat ttttggaaga ggcgtgtgca aataaattat caccaatata aattccaacg
     1081 tgcccaggtt taactctttt gcgcgtattg aaaaaaacaa gatcaccaaa ttttaattca
     1141 gatctgtcat caattacaat tccctgctga tattgttctc ttgctgagcg attaagctct
     1201 aacatccacg aatccctgta tacattctgc gtgaaagcac tgcaatcaat tccattcaac
     1261 gaattacctc catacttgta cggagtatta agatacttga ttatttccat caacattaat
     1321 tctctttttg tagatacgaa agtctcgcca tcggaaccgg tggatttctt atccaggttt
     1381 ttcatcaatt cggtcaaatc aattttagct tcatcttcag gtaaatcact gggatcctga
     1441 aattcagaaa tatcatcatc aattactgct aaagtatcat caacagatgg ggtctctgtg
     1501 gaagtatatc gtgtgttagt agtttgtttt gtcttttcgt tttcgttatt actattatat
     1561 cttaatgaac tggatgagcc tgaacaggca attaaagtaa gtgaactaat caaaataaac
     1621 acatataaaa aatatttttt taaaactgtc atttactatc caagatatga ttttaagatt
     1681 ttacttctgg aattatgcct taatcttctg atcgcttttt cttttatctg tcttactctt
     1741 tccctggtaa gattaaactt atcaccaatt tcttctaatg taagaggatt ttcttcatcc
     1801 aatccaaaat acaatttaat cacttctgct tcccgctcag gaagtattga aagaacttct
     1861 tttatttcgg cttttaaaga ttcagaaata agttctgtat ccggtgctgg catttcatca
     1921 ttttccatta catccaataa gctgttttca tcgcccaaag aaaatggagc atcaacagaa
     1981 acctgcttgc cataaatttt cattgtattt gaaatttcat caacatccat ctcaagtgct
     2041 tgagcaattt cctcagaagt aggttctctc tcaaattcct gtttaagatt actatatgtt
     2101 ttaccaattc gatttatcgc accaatttga tttagaggaa gccgcacgat tcttgactgc
     2161 tcagctaaag cctgtaaaat agcctgcctt atccaccaaa cagcataaga aataaatttg
     2221 aacccgcgtg tttcatcgaa gcgtcttacc gctttaatta atccaacatt accttcatta
     2281 attaaatcac caagtgatag accctgattt tgatattgct tagcgacact gaccacaaaa
     2341 cgtaaattag cttttgtaag aagttccatg gcagaatcat caccattttt cattcgaact
     2401 gtaagttcaa tttcctgttc aggagtcagc aattcaactt tacctatctc ctgaagatat
     2461 ctgtccaagg attggttatc ccgatttgta aattgtttaa ctatcctcat tttgtcatct
     2521 taaaaatgtt taactgagca aataacaata aatattcaag tcgtaatcat gatactatta
     2581 aataaacttt ggtttaatta aaaatagata ggaaaagatt taaaataaag aggcaacttc
     2641 attcataaat ctgcaattag ctattctatg gtttgaggat gtttcgaatt tactcttaaa
     2701 ccgtaatatt tatcgggatt taaagttacg atatctaatt tgttttgctc agctaatatg
     2761 tagaaaagtg cattccgggt ggatttaaaa ttatttttac acgcaggaat tgacaaacta
     2821 taacgagcat gtaatccaag aactttaagg gcatttaata gatctctgac cctttctttc
     2881 tccaaatctg tatctgccat cataaataat tctgaagcat ttaatactga agtaaaacag
     2941 attcccctct gcattagctt tatgagatat ggttctttat tagaattatg aatggttaaa
     3001 taatcaacaa gaatatctgt ctccaacaaa aactttaaag gtttatctaa ggtcatctga
     3061 atatttttta ccaaaatcag tagaagaaag tgaaccggct gctttcaaaa attttgttac
     3121 tacctcttca ggtaaatcct gaatttgaat tatccgaaat aataattcct tttcaaatac
     3181 ctgctcatca cccggattat tttttacatt ttcagaatta ttcatttaaa aagcttgtgc
     3241 ctttcaaatc attttttact ttaaaataat cggcagccgt ttttccgaca tcagcaaatg
     3301 tgtttctgat gcctaaattt tttgcagatt tattttttcc gaaatatata accggaacat
     3361 attccctgct atgatctgta cttggagttg ttggatcgtt tccatgatca gctgtaaaaa
     3421 tgactcggtc tgtcggatca agattttcta gaaattcagg tagaaaatca tcaaattctt
     3481 ttaaagctat agcaaatcct tcaaaatcat ttcgatgtcc aaaataaaca tcaaaatcaa
     3541 caagattaat aaatattaaa ctttgttgat attcgtttga ggcctttatt agcttctcaa
     3601 aaccctctct attagatttg gattttactt ggattgatat tccgcgatag ttaaaaagat
     3661 cattaatttt gccaattgca actgtgttaa ttatattttt ttgcaaaaga tccagtacag
     3721 tttctgatgg tggatctaaa gaataatctt ttctataaac tgtcctttcg taactcccgc
     3781 ttttaccaat aaaaggtctt gcaataactc taccgacttt taagggatta attaaaactt
     3841 tatttcttgt tttatcacag atctcataaa gtttttctaa aggaataacc tcctggtgtg
     3901 ctgcaatttg aaaaacagaa tcggctgaag tatatattat tggaaagccg gttcttaagt
     3961 gttcatcacc caatacttta ataatttctg tacctgatgc agctttgttt ccgagatatc
     4021 ctttgcatcc tgtagtttct aaaaacttat ctaaaatatt tttaggaaaa ccatttggga
     4081 aataatcaaa atcagtatca acatacaaac cgcttaattc ccaatgcccg gttgtgctgt
     4141 cttttccttt tgatagttca ctcattttac caaacgaagc taa
//
LOCUS       DASWBY010000631         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_740871, whole genome shotgun sequence.
ACCESSION   DASWBY010000631 DASWBY010000000
VERSION     DASWBY010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740871"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VF870_11485"
     CDS             complement(<1..1058)
                     /locus_tag="VF870_11485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9252857.1"
                     /translation="MKNNVLSFNVFKKLFITGLIYLSIINSTYSQTNSGAAPPLTKDN
                     VETFLDSIITSLMKEQNVLGCGIAIVKDTSILFSKGYGYADIEKKKPFDSEKTIFRTA
                     SVCKVVVATVLMQLKEKNLIDLNADINKYLKKFKIKDKFGKPITTANLLTHTPGFDDF
                     YIGKSARTEKEAMPLGEFLRKHMPERIVPPGEVSMYSNMGMALAAFLVEEITGTDFEQ
                     YTIENLFLPLDMNKSSFRGKEKYKDDIYKGYVYLDGKQNEFPFDFINDYPAGQMLTTI
                     NEFSNFMIMHLNNGKFGGRKIVYSLIIDEMQSVQFTHHPKLHSALGYGFFIDEVNGTR
                     LLSHNGGYPGILTRMLIFP"
     gene            complement(1268..1687)
                     /locus_tag="VF870_11490"
     CDS             complement(1268..1687)
                     /locus_tag="VF870_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252858.1"
                     /translation="MKKAVIVIQNKNSTTKKYAEEIAQFLLNRGLTAELIPINSFEPK
                     KLEEADYLLVSGWKNGSFFSAKRPDSEWVSFVNKLPTLNGIKTALFTTYNLFSGGIVK
                     SMKKYLGRKTNNLEFAFTSRDGSLTISDKLTLNDFIG"
     gene            1988..2647
                     /locus_tag="VF870_11495"
     CDS             1988..2647
                     /locus_tag="VF870_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252859.1"
                     /translation="MKEELERIIKDMGHNDLAPIRNSQNGEKEKFSELFNRLKQFDEI
                     SQSNKYDLLSIPPAIRMVLKIFPHKYCNDNSLKVKFLRAFVTTVIPGANENQDNLVSM
                     YLDSYYPFQTYCGYFVYDLTRRSKKLFNNKEFFNLSEDEKVQVINNALNGRELTRRLY
                     KGAILMAQVSYYGAVYDEEKGCPLIDFPGRNNGYNKEEIAYPYSSTLFDKELSLDGHP
                     W"
     gene            2673..4199
                     /locus_tag="VF870_11500"
     CDS             2673..4199
                     /locus_tag="VF870_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9252860.1"
                     /translation="MKTLNTEVLIIGSGFGAAPPALHFCERGYDVLLIEKGDNITPES
                     DFKQTMDPKYLLKYIKSLKGDGIQFTFAEGLGGGSGFYEMVSFRAPSQAFDLKNENGK
                     RLWPTGFNRKSLDPYYQIAEEKLNVHQIEKDCIPKNGVVFCKLMRNLGYSVDRVNYSV
                     KDCRGSSFCVAGCKFGAKQNLITTYLRKAEQLGLNKIANIQAKEIIPLNNNGHYSEEL
                     RNINKTPFNYQVNACSNPSGEKIQIFTKILILAAGTVGTAILLLNSKKYFNNISDHIA
                     KNINVNGSIKTVGILPDDFIEGDMFVGMSHPGVVSYEFLDSRGITISTSKPLPVDAVS
                     TAHFSIEGENGNSSWWGKKQVEFMKKYRRKALVLYALGLSTSTAELRKNGNGSYKPYF
                     NIDEKFREYNESTTQLLHSIFRRNGAKVVNLNFIDGEGNEYHDIHIFTAHMTGSCRMA
                     DDINQGVVDVNGEMFNQPGLFITDGATIPSSLAVNPYLTILANAERLGNTITGMYSRN
                     "
BASE COUNT         1396 a          700 c          764 g         1383 t
ORIGIN      
        1 ggaaagatta acattcttgt taaaataccg ggatatccgc cattgtgtga taaaagtctt
       61 gttccattaa cttcatcgat aaaaaatcca taacccaatg ccgaatgaag tttaggatga
      121 tgagtaaatt ggactgactg catttcatct ataatcaaac tataaacaat ttttcttccc
      181 ccaaattttc cgttattcaa atgcattatc ataaagttgg aaaactcatt aatcgtggtg
      241 agcatttgtc cagctggata atcatttata aaatcaaacg gaaactcatt ctgctttccg
      301 tccagataaa catatccttt ataaatatca tctttatatt tttcttttcc tctgaaagaa
      361 cttttattca tatcaagagg aagaaagaga ttttcaattg tatattgttc aaaatctgtt
      421 cccgttattt cctcaacaag aaaagcggca agtgccatac ccatatttga atacattgag
      481 acttcaccgg gaggcacaat gcgttccggc atatgttttc taaggaactc tcctaatggc
      541 attgcctcct tttccgtacg ggctgatttg cctatgtaaa aatcatcaaa gccgggagtg
      601 tgtgttaata aatttgccgt tgttattggc ttaccaaatt tatctttaat cttaaatttt
      661 tttaaatact tgtttatatc cgcgttaaga tcaattagat ttttctcttt aagctgcatc
      721 agcacagttg caacaacaac tttgcacaca gatgccgttc tgaaaattgt tttttcagaa
      781 tcaaatggtt tctttttctc aatatcagca taaccataac ctttagagaa tagtatgctt
      841 gtatctttta caattgcaat cccgcagcct agaacatttt gctccttcat caaagacgtg
      901 attattgaat ccagaaaagt ttctacgttg tcttttgtta aaggtggtgc ggcaccagag
      961 tttgtttgag aatatgttga gtttattatt gaaaggtata tcaacccggt gatgaataac
     1021 ttcttaaaaa cattaaaaga cagaacatta ttcttcatat tttatttccg taaagtgtct
     1081 gcagaatatt ttatgtttct gatgtaaact taattaaagc tgtcttgtta gaaaataacg
     1141 ctgcggtttt taagtgagaa ttgtttaaca gtagttgttt gaattgtcct gcgaactttg
     1201 tggtaaaatc tttaccacaa agaacacagg ttaatcaata aaaaggaatt tggcggaaat
     1261 agatttacta tcctataaaa tcgtttaatg tcagcttatc agaaattgta agactgccat
     1321 ctctagatgt aaaagcaaat tctaaattgt tggttttacg tcccagatat tttttcatgc
     1381 tttttacaat tccaccagaa aacaaattat aggttgtaaa caaagctgtc tttattccat
     1441 tcagtgtcgg cagtttattt acaaagctta cccattcgct gtctggacgc tttgcagaaa
     1501 agaatgaacc gtttttccat ccgcttacca gcagataatc tgcttcctca agtttttttg
     1561 gttcaaagct gttaattggt ataagttccg cagttagtcc tctgttaagc aggaactgtg
     1621 caatttcttc agcatatttt tttgttgtgc tgtttttatt ctggataact atcactgctt
     1681 ttttcatttc tacacctttc agaaaatctt ctataagaaa cataaaaagc atcatagtta
     1741 aacactatca acttattatg agcggggatt tttagtgatt tgtgccggat atctgttgtt
     1801 aaatatgtag cattagtata tctgatattc tgcaacaaat catttttcct catctgttta
     1861 tttttattca aataatcttt tttacaaatt cttgaaactc tttttacaac tctatgacat
     1921 ctttcgctgt ggtataagct ataataatat cagaaaagaa ataattatta accacaagga
     1981 gaatgaaatg aaagaggaat tagaaagaat aataaaggat atggggcata acgatttagc
     2041 cccaataaga aactctcaaa acggtgaaaa ggaaaaattt tctgagctat ttaatagatt
     2101 aaaacaattt gatgagattt ctcaatcgaa taagtatgat ttgttgtcta ttccacccgc
     2161 cataagaatg gttctgaaga tatttcctca caaatattgt aacgacaatt cacttaaagt
     2221 aaaattttta agggcatttg ttacaacagt aattccggga gctaacgaaa atcaggacaa
     2281 tctggtaagc atgtacttag acagctatta tccatttcaa acttactgcg gttactttgt
     2341 ttatgattta accagaagga gcaaaaaact atttaataat aaagagttct tcaacctttc
     2401 tgaagatgag aaagtacaag taattaataa tgcccttaac ggaagagaat taacaagacg
     2461 cctttataaa ggggcaatcc ttatggctca ggtttcatat tatggtgctg tatacgatga
     2521 ggaaaaaggc tgtccgctaa tagattttcc gggcagaaat aatggttaca ataaagaaga
     2581 gattgcttac ccatactcaa gtactctttt tgataaagaa ctgtcgttag atggtcatcc
     2641 atggtaataa ctaaaataga atggagaaga aaatgaaaac gttaaacact gaagtattaa
     2701 taatcggaag tggttttggt gctgcaccgc cggcactgca tttctgcgag cggggttatg
     2761 atgttttatt aattgaaaag ggagataaca taactccaga aagtgacttt aaacaaacta
     2821 tggatcccaa atatcttctt aagtacataa aatctctaaa aggagacggg attcaattta
     2881 cttttgccga gggattgggc gggggttcag gtttttatga gatggtttct ttcagagcac
     2941 cttcgcaggc ttttgattta aagaacgaga atggtaaaag attatggccg acaggtttta
     3001 acagaaaatc tcttgatccc tactatcaaa tagctgaaga aaaattaaat gttcatcaga
     3061 ttgaaaagga ttgtattcct aagaatggtg ttgttttctg caaacttatg agaaaccttg
     3121 gatattccgt tgaccgggta aactattctg ttaaggattg tcgcggctcc agtttttgtg
     3181 ttgccggatg caagtttggc gcaaaacaaa atcttatcac aacttatttg agaaaagcag
     3241 aacaactggg tttaaataag attgctaata ttcaggcgaa agaaataata ccattaaata
     3301 ataacgggca ttactctgaa gaactgcgca atattaacaa gacacctttt aattatcagg
     3361 ttaatgcatg ttcaaatcct tcaggagaaa aaattcagat ttttacaaag attttaattc
     3421 ttgcagcagg aacagttgga acagcaattc ttttactcaa ttcaaaaaaa tatttcaata
     3481 atataagtga tcatattgct aagaatataa atgttaatgg tagtataaaa acagttggca
     3541 ttcttcccga tgattttatt gaaggagata tgttcgttgg tatgtctcat cccggagttg
     3601 tgagttatga atttcttgat tcaagaggta ttacaatttc aacatctaaa ccattgcctg
     3661 ttgatgccgt atccacagcc cattttagca tagaaggtga gaacggaaat tcatcatggt
     3721 ggggaaaaaa gcaagtcgaa ttcatgaaaa agtacaggag gaaggccctg gtactttatg
     3781 cgctgggtct atcaacctca actgcggaac ttagaaaaaa tggtaatggc agctataaac
     3841 cttacttcaa tattgatgag aaattcagag aatacaatga aagtacaaca cagctgcttc
     3901 attcaatttt tagaaggaat ggtgcaaaag ttgtaaacct caattttatt gacggcgaag
     3961 gaaacgaata tcacgatatt catattttta ctgcccatat gacaggatct tgcaggatgg
     4021 ctgacgatat aaaccagggc gttgttgatg tgaatggtga aatgtttaat cagccgggtt
     4081 tatttattac tgatggcgca acaattccat catcgcttgc agttaatcct tacttaacta
     4141 ttcttgccaa cgcagaaagg cttggtaata cgataaccgg aatgtacagc aggaactaat
     4201 atgagcattt tattaacagg cgcaggtggt tttataggag gct
//
LOCUS       DASWBY010000632         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_621872, whole genome shotgun sequence.
ACCESSION   DASWBY010000632 DASWBY010000000
VERSION     DASWBY010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621872"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..>1683)
                     /locus_tag="VF870_11505"
     CDS             complement(373..>1683)
                     /locus_tag="VF870_11505"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX9252861.1"
                     /translation="TLPLADHSYLGILIGQKLDKSEFTNLDKITLQILLQVFDSAYKS
                     FLNHKKEKALIFELNEKVLHLNNLVDTGIEISRFQKKRVLYDVALERITSLTNASSAC
                     LEIIKKYHDNIKKHITFPEGIAPEKILNNKFRIQSSFEHEDRIYNFILSEKETRHNTT
                     TFNELDQVLLDSIARQVYTSLENESLHKQALEKEKMEQELTVAASIQQRILPTELPEI
                     KGYNLSGINIPSREVGGDYYNCFKLKNGKYALIIADVAGKGIGAALLVSTLDAALYSY
                     LEFDIPLTEMADRLNKLIYKSSPSDKYITFFIAVLDAESGELDILNAGHNPIFLLREN
                     GTLEKLDAGGVGLGMFDFGIPFAGQKSIMNPGDKLFLYTDGIPEAMNEKEEEYSDEKM
                     LNFFIEHSNKSSEDFINALVIDVKNYAGAALQSDDITVMILKRI"
BASE COUNT          524 a          297 c          262 g          600 t
ORIGIN      
        1 tgacgatcgt tgatgtgtgt tgcagaaccc agtaatgtca tttcgaccga atggaaaatt
       61 ttttttgtag agagcatcag gtttctcagt cgtcaaagca ctccttcgta atgaaatttc
      121 tgaattaaac aatactcctt aaatttcttt gctgctgaat attcttactt aacattaatt
      181 acaactatat cgtgtgtaat cattatatct atcagctgag tgaacattca tacctacacc
      241 tcgactacgc tcagtgtgat gtttaattat tatcactcaa tatattttta gtaaagtaat
      301 cttaaagcta aggtttaaaa ttatttagag attactttga tactgccagc acccgtaata
      361 acaatttata acttaaattc tttttagaat cattacagtt atatcatcgc tttgcaaagc
      421 tgcacctgca taatttttta catcaataac cagagcattg ataaaatctt cagaggactt
      481 attggaatgc tcaatgaaaa agtttaacat tttttcatca gaatattctt cctctttttc
      541 attcattgct tccggaattc catctgtgta aaggaaaagt ttatcaccgg gattcataat
      601 tgatttttgt ccggcgaatg ggattccaaa gtcaaacatt ccaagtccca caccaccagc
      661 atcaagtttt tccaaagtac cgttttctct taaaaggaaa atcggattat gccctgcatt
      721 taaaatgtcc agttcaccag attcagcatc gagaactgca ataaaaaatg taatgtattt
      781 atcggaggga gatgatttat aaatcaattt atttaatcta tcagccatct ccgttagagg
      841 gatatcaaat tcaagatatg agtagagagc cgcatctaaa gtactaacaa gtaaagctgc
      901 accaattcct tttccagcaa cgtcagctat aatcaaagcg tacttaccgt tttttaattt
      961 gaaacagttg tagtaatctc cgccaacttc tcttgatgga atatttatac ccgaaagatt
     1021 atagccttta atttcgggta attctgtcgg cagtattctc tgttggatag aagctgcaac
     1081 ggtcagctct tgttccattt tttctttttc taaagcctgt ttatgcaagg actcgttctc
     1141 taatgaagta tatacttgtc ttgcaatcga atcaagaagt acctggtcta attcgttaaa
     1201 agttgtagtg ttgtgcctgg tttctttttc tgaaagaata aaattataaa tacgatcttc
     1261 atgctcaaat gaggattgaa ttctgaattt attattcaat attttttccg gtgcaattcc
     1321 ttcaggaaat gtaatgtgct ttttaatatt gtcgtggtat ttttttataa tctcaagaca
     1381 tgcagaagat gcattggtaa gtgaagttat tctttccaga gctacatcat acaaaactct
     1441 ctttttttga aatctggaaa tttctatacc ggtatcaaca agattattaa gatgcagcac
     1501 cttttcattt aactcaaata tcagtgcttt ctctttttta tgatttagaa atgacttgta
     1561 agcactatca aatacctgca atagtatttg taaagtaatt ttatcaagat ttgtgaattc
     1621 tgatttatct aatttctgac cgataagaat tcccaaataa gaatgatctg caagcggtaa
     1681 tgt
//
LOCUS       DASWBY010000633         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_211690, whole genome shotgun sequence.
ACCESSION   DASWBY010000633 DASWBY010000000
VERSION     DASWBY010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211690"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VF870_11510"
     CDS             complement(<1..548)
                     /locus_tag="VF870_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX9252862.1"
                     /translation="MLIGIIANITKENVFKVVSSFISKLKKNNLDYILTKSLSEDKDK
                     IKIELFDDFVTDDKEVYEKADLIISIGGDGTMLATAFNAQFYDKPVLGVNMGKLGFLV
                     EANIDQMDKIITELKDGKFSIEERMVISAEALGYEKEKLYAINDIVIDKGGWPKMIEL
                     TVWVDGEYVTTFSADGLIVATPT"
     gene            complement(643..1758)
                     /locus_tag="VF870_11515"
     CDS             complement(643..1758)
                     /locus_tag="VF870_11515"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9252863.1"
                     /translation="MKVLYDHQCFVQQQFGGVSRYHYQLIKELNKLQDVNAKLSLKYS
                     NNFYINEDRNFEVKRFFPNHKLYFKRTILDLINRISTSRMLKKGDYDIFHPTYYNPYF
                     LDHLNNKPFIITVYDLIQEKFPEIINSIDKTLENKKAVLSKAQIILAISNSTKNDLIQ
                     LYGISAEKIEVVYLAASINKTFASANLKLSLPEKYILFVGNRDFYKNFKNFVLAVEPL
                     LKEHKDLFLIAAGGGGFTKEENQHFNSMHMDKKIIHFGADDKTLAALYSNSLAFIFPT
                     LYEGFGIPALEAMNCDCPVIMSNTSSLPEVGGDAAIYFDPNNTDDMREKIESLIINKD
                     LRDDLILKAKTQRQKFSFEKTALQTLEVYKKLIEIEL"
     gene            complement(1773..>2418)
                     /locus_tag="VF870_11520"
     CDS             complement(1773..>2418)
                     /locus_tag="VF870_11520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9252864.1"
                     /translation="EAYSRSINLSDTGLLDRNIYFSKILSLLIFFCFNKNGSFLDYAG
                     GYGVFTRLMRDIGFDFYWHDPYTQNLFANGFEGDLKNNSKYELLTAFEVFEHLVNPKE
                     ELERMLCLSDTIIFSTELMPQEIPDPKDWWYYGFNHGQHISFYTERTLSTLAHQNKLN
                     YYNVEGVHILTKRNFYRTLLILMKKFRNYGLYQIVKKLVKSKTFSDHLNIDNKS"
BASE COUNT          786 a          412 c          344 g          876 t
ORIGIN      
        1 gttggagttg caacaattaa accatcggct gagaaagttg taacgtactc accatctacc
       61 caaacggtaa gttcaatcat tttaggccag ccgcctttat caattacaat atcatttatt
      121 gcataaagtt tttctttttc ataaccaaga gcttctgcgc taataaccat tcgctcctcg
      181 atactgaact taccgtcttt caactcagta ataattttat ccatctgatc aatgtttgct
      241 tcaacaagaa atcccagttt tcccatatta acaccaagca caggcttatc ataaaactgg
      301 gcattaaaag ctgttgcaag cattgtccca tcgccgccaa ttgagattat taagtcagct
      361 ttttcataaa cttctttatc gtctgttaca aaatcatcaa agagttctat ttttattttg
      421 tctttatctt cagaaagaga ttttgtaaga atataatcga gattgttttt tttcagcttg
      481 gagataaaag atgagacaac tttaaaaaca ttttctttgg tgatattggc aatgattcct
      541 attaacatat ttttctcaaa gttgtttttg tacggctata aattaccact ttttctgtaa
      601 agaaattttc agattatgta gaattttatt aataacgata tactataatt caatttctat
      661 taatttttta taaacctcca atgtttgtag tgctgttttc tcaaatgaga atttttgtcg
      721 ttgagttttc gcttttaaga taagatcatc tcttaaatct ttattaatta ttaatgattc
      781 aattttttct ctcatatcat cagtgttgtt tggatcaaaa taaatcgctg catcaccgcc
      841 aacttccggt aaagaacttg tgttactcat aattacagga caatcgcaat tcattgcttc
      901 caatgcggga attccaaaac cttcatatag agtcggaaaa ataaacgcaa gtgagtttga
      961 atataaagct gccagagttt tatcatctgc accaaaatga attatttttt tatccatatg
     1021 cattgaattg aaatgttgat tctcttcttt agtaaaacca cctccaccgg ctgcaattaa
     1081 aaataaatct ttatgctctt taagcaaggg ctcaacagca aggacaaaat ttttgaagtt
     1141 tttataaaaa tctctgttgc caacgaacag aatatatttt tccggtaaac ttaattttag
     1201 gtttgctgat gcaaaagttt tattaattga ggccgccaaa taaacaactt caattttctc
     1261 agcagaaata ccgtaaagct gtattaaatc attttttgtg ctgtttgaaa tcgcaagaat
     1321 tatttgggct ttagaaagta ctgctttttt attttctaga gtcttatcaa ttgagtttat
     1381 tatttcagga aatttttcct ggattaaatc ataaaccgtt ataataaaag gtttattgtt
     1441 taaatgatcc aagaaatagg gattataata cgttggatga aaaatatcat aatcgccttt
     1501 ttttagcatt ctgctggttg agatgcgatt aattaaatca agaatagttc ttttgaaata
     1561 gagtttatga tttgggaaaa acctttttac ttcaaaattt ctatcttcat taatataaaa
     1621 attatttgag tatttaagtg aaagctttgc attaacatcc tgcaacttat ttaattcttt
     1681 tatcaactgg taatgatagc gtgacacacc gccaaactgc tgctgtacaa aacactgatg
     1741 atcgtataaa actttcacta aataatgcct cgttaacttt tgttatcaat attaagatga
     1801 tcgctgaatg ttttgctttt tactaatttc tttacgattt gatacaaacc atagttcctg
     1861 aacttcttca ttagaatcaa taaagttcta taaaaatttc gtttagttaa aatatgtacc
     1921 ccttcaacgt tgtaataatt caatttgttt tgatgtgcta aggtgctaag cgttctttct
     1981 gtataaaacg aaatgtgctg cccatgatta aaaccataat accaccaatc ctttgggtcc
     2041 ggtatctcct gcggcattaa ttctgttgag aatattatag tatcgctaag acatagcatt
     2101 cgttccaatt cttctttagg atttaccagg tgttcaaata cttcaaatgc tgtaagcagt
     2161 tcatattttg aattattttt tagatcaccc tcaaatccat tagcaaataa attttgtgtg
     2221 taaggatcat gccaatagaa atcaaaacca atatccctca tcaaccgagt aaatactcca
     2281 tatcctccag cataatccag gaaagatccg tttttattaa agcaaaagaa aatcaataat
     2341 gaaagaattt tggaaaagta tatattacga tctagcaaac ctgtgtcgga aagatttata
     2401 cttcttgaat atgcctca
//
LOCUS       DASWBY010000634         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_662626, whole genome shotgun sequence.
ACCESSION   DASWBY010000634 DASWBY010000000
VERSION     DASWBY010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662626"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VF870_11525"
     CDS             complement(<1..805)
                     /locus_tag="VF870_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9252865.1"
                     /translation="MNDIKIDVDLTNDTLEILKKYTIRIPEHYFGIDRVIFLNSVMKD
                     ISLEENRVLNNYINYLLRIMLMREASDLELGGFGSDGFVWLRVYGAKYILSELPKLTF
                     DESALLILNIITEKQRGLLIKNRNLDFSYSFNHEKGLSQGLKLESEFIRFRADAYFDV
                     DTLALNIRSILPKLRKLENLGFHPNVIRVLSFNYVKQGLVLVTGVTGTGKSTTLDAIV
                     DWHNSNVNAHIVTIASPLEYVHRSKKCIIRQREVGKDVLSFREGVVQALR"
     gene            complement(845..>1893)
                     /locus_tag="VF870_11530"
     CDS             complement(845..>1893)
                     /locus_tag="VF870_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX9252866.1"
                     /translation="QQNTIPEAIIAVVKDRSRGLDRFERESGQDGFIQREIDGHIIRF
                     RVSIIPIAGSEIRNKFESIVIRILDDRKVIKDLGALGFSGYALTSFKKAINNPQGLVI
                     LTGPTGCGKSTTLQAALHQVINPSVNVITVEDPIEYIIKGARQVKLGHKLDFEHALRY
                     ILRHDPDIVLVGEMRDKETADIAIKLANTGHLTFSTLHTNDAPSAVSRLFKMGIEPFL
                     LANAINIIVAQRLIRTLCECKRKSDKYDEKSLKRLGLKPAEWNNIDLYEPVGCEKCLG
                     SGFKGRMAVHEAIYITKEIAQIILDAGDKIDEGSVRKAAKKDGSLTLREAGLERVKLG
                     VTSIEEVLASTMDD"
BASE COUNT          579 a          374 c          285 g          655 t
ORIGIN      
        1 gtcgtaatgc ctgaactaca ccttcgcgga aagaaagaac atctttccca acttctcttt
       61 gtctgatgat acacttcttg gaacgatgca catattccag aggtgaagca atagtaacaa
      121 tatgtgcgtt cacattgcta ttatgccaat ctacaatagc atctaatgtt gttgattttc
      181 ctgtacccgt aacgccagtt actaatacca gtccttgttt gacataatta aatgaaagga
      241 cacggataac attcgggtga aaacccaagt tttcaagttt tctaagtttt ggtaaaattg
      301 atcggatatt tagtgcaaga gtgtcaacat caaaataagc atcagctcta aatctgataa
      361 attctgattc taatttaaga ccctgagata aacctttttc atgattaaaa gaataagaga
      421 agtcaagatt tctattttta attaataacc ctctttgctt ttcagtaata atgtttaaaa
      481 tgagcaatgc tgattcatca aaagtaagct ttggaagttc tgatagaatg tactttgctc
      541 catatactct tagccagaca aaaccatcag aaccgaaacc acccaactct aaatcggatg
      601 cttcccgcat taacataatt cttaataaat aatttatata attatttaaa actcgatttt
      661 cttcaagaga aatatctttc ataactgagt taagaaagat cactctgtct attccaaaat
      721 aatgttccgg aattctaatt gtatatttct ttaggatttc taaagtatca ttagttaaat
      781 ccacgtcaat tttaatatca ttcactaaca ttttattatt tcatttatag ttgatataaa
      841 ataattaatc atccatagtg ctggctaaaa cttcttcaat tgaagtaacg ccaagtttaa
      901 ctcgttctaa tccggcttca cgtaaagtta atgatccatc ttttttggca gcctttctta
      961 cgcttccttc gtcaatttta tctccggcat ctaaaattat ttgagcaatt tctttggtga
     1021 tataaattgc ttcgtgaaca gccattcttc ctttaaatcc tgagcctaaa catttctcgc
     1081 atccaaccgg ttcataaaga tcaatattgt tccattcagc tggtttaaga cctaatcttt
     1141 tcaaagattt ctcatcatat ttatcacttt tccttttaca ttcgcaaaga gtacgaatca
     1201 atctttgagc tactataata tttatcgcat ttgccagaag aaaaggttcg atacccattt
     1261 taaataaacg ggagactgca cttggtgcat cgttagtgtg cagggtagaa aaggttaagt
     1321 gtccggtgtt ggctaacttg atcgcaatat cggctgtctc tttatcacgc atctcaccta
     1381 caagtacaat gtctggatcg tggcgcagga tatatcttaa tgcatgttca aaatcaagtt
     1441 tatgtccaag ctttacctgt cttgcacctt ttataatata ttcaattggg tcttcaacag
     1501 ttataacatt aactgaggga tttataacct gatgaagagc tgcctgtaat gttgtacttt
     1561 taccgcatcc cgttgggccc gtaagtataa ctaagccttg tgggttatta attgcttttt
     1621 taaaactagt taaggcgtag cctgaaaatc ctagtgcgcc caaatcttta attacttttc
     1681 tatcatccaa aattctaatt acaatacttt caaacttatt gcggatctca cttcctgcaa
     1741 ttggaataat agagactcta aatcttatta tatgtccgtc aatttcacgc tggataaatc
     1801 catcctgtcc gctctctctt tcaaatctat caagtcctct tgatcggtcc ttaacaactg
     1861 cgataatagc ctcaggaatt gtgttttgct gtc
//
LOCUS       DASWBY010000635         8974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_362132, whole genome shotgun sequence.
ACCESSION   DASWBY010000635 DASWBY010000000
VERSION     DASWBY010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362132"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VF870_11535"
     CDS             <1..962
                     /locus_tag="VF870_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252867.1"
                     /translation="GRQPIINSIAGGVYDGASLTLKKTDYKFNAYFGGNVPAYQKFEL
                     IENFGENYLFGGKFTTTVLHNFQLGLGYVNKNFQPQNYTADRLKADLTPIEVEIQNKS
                     NQYEFVTGEVNYNQQGLVSIFTRYDYDLNFNQTSKFEFDARADAVKDWAINLYFNYRQ
                     PKIRYNSIFAVFDYGNTYEVEVGTDYKINSMFTVTGKFGNVQYKDENAQRIGLGLATT
                     YGSINYRKTFGYAGELDAISLYSGYTFFEGLFTPSIGLSYTNYKLSPDDEANNITTLM
                     VGANIRPFRTLSFDLQGQYMDNKIYKNDMRLFLKLNYWFNLNI"
     gene            973..2187
                     /locus_tag="VF870_11540"
     CDS             973..2187
                     /locus_tag="VF870_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9252868.1"
                     /translation="MQKLKVLYLFVFLGFVIFLLVGAFSTQKDDKPKTNEGRIKFSHS
                     LHNELIDCQTCHAAVSESVSLKDPLFPNHDVCSSCHEVDNEDECSTCHYDDNYEALVK
                     SEKGLIFNHKLHLADKGTNCESCHKGLSEVDYSWQSADAHPPMETCYSCHNDKTVASN
                     ACEGCHISTVNLLPQDHKVVSFAKSHKFAAQEFNANCVMCHDNQSCEDCHVATIGITE
                     TNTLNNFYQPYYPSNFVDGAKVQAINRVHELNYRFSHGIDSRGKTAECQTCHQIETFC
                     AECHQAEDQDFAFGGIVPASHLKSSFKTIGVGSGGGDHAILAKRDIESCMACHDVNGA
                     DPTCITCHLDSDGIKGTNPKTHAAGFMRDEHGDWHNDNSSICYNCHTSASPQSQPGLG
                     FCGYCHGTNVND"
     gene            2215..3735
                     /locus_tag="VF870_11545"
     CDS             2215..3735
                     /locus_tag="VF870_11545"
                     /inference="COORDINATES: protein motif:HMM:NF021234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HEX9252869.1"
                     /translation="MKYLILFITLFLSSVLFISCSDLKNDIPLTPDINIHGSEVFDST
                     ASNFHGKQVLDSQNSFQDCKQCHDANYSGGIAKVSCYSSDCHVSPAINVHEAGITDVQ
                     SPNFHGKFIADKVNGSMISCAQCHGDSYQGGVVSPSCANCHSGIPVHVGDYVNPISPN
                     FHGKFIADKVNGSMVSCAQCHGDSYQGGIASPSCANCHSGIPVHVGDFVNPSSPNFHG
                     KFIADNFSGSMNSCAQCHGDSFQGGVASPTCTNCHSTIPVHVDGIVNPSSPNFHGKYI
                     VANLAWDMRACGSCHSSDYSGGIAAPSCLTCHTSTNGPEACNTCHGDFNDPTKIAPPR
                     ALNGSAVTTYAGVGAHTSHLITNNLGSDVSCSTCHTYPQSVYANGHLGSDGKAEIHFG
                     NLATHNGINPTYSFSNNTCSDTYCHGNFAFYRDSSNFAFAYTAATMEGSNFSPKWNQV
                     DGSQAVCGSCHGLPPVGHIPAELSTCVNCHPGVVDGQGNIVDRTKHINGVKNVFGN"
     gene            3796..4419
                     /locus_tag="VF870_11550"
     CDS             3796..4419
                     /locus_tag="VF870_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252870.1"
                     /translation="MKIFNNNKTKNEANGNISLSDYERKLEERKEEYEHLNEILRGMR
                     AEVSNLMEEIKNVTDNLVKKNAELFLLEQSIDDLKRSYELLQQNVKYEEEAFSKVNNE
                     LTKIKNEITESLPYHNKIISAKNEFEILSKQLIESKAEIEKTNNQTKLLGEISENVEL
                     KNELDIPIATKKQKCHAKTKTGSKCKRYAVEGSLYCASHSSIVNEPN"
     gene            4519..5148
                     /locus_tag="VF870_11555"
     CDS             4519..5148
                     /locus_tag="VF870_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX9252871.1"
                     /translation="MNGFTHFIGIILAIIATILLINLSLNPYRPMHLISFTVFGFGMI
                     LLYTISTLYHWLKLSDAGILKLRKADHIMIFIYIAATYTPICIIALKGTLGWGLFGAV
                     WFVALGGIIIKIFWMKAPRWISTLIYILMGWLAVIVIYPIVNSLPTEALIWLLIGGLF
                     YTIGAVIYAVKKPDPFPGVLGFHEIFHVFVLLGTFSHFWMMYKYIAVMN"
     gene            complement(5162..5683)
                     /locus_tag="VF870_11560"
     CDS             complement(5162..5683)
                     /locus_tag="VF870_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252872.1"
                     /translation="MEKAISVIDFKSNAKTYLIDFSLLLIIYFLPALSHLFAFPVYYF
                     DPMRIALVVALIHTSKKNAYIIALTLPLFSFFISSHPQILKTFLISTELIVNLSLFFL
                     LKNRLKNVFVSLFVSIIISKVIYYFLKFTLISSSLLDDKLFSTPFYFQLISAIVLSTY
                     IYLINRNQLNNHS"
     gene            5824..6747
                     /locus_tag="VF870_11565"
     CDS             5824..6747
                     /locus_tag="VF870_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX9252873.1"
                     /translation="MRRRDFISKSFKASLIAGAGLSVGSYSNLFPSAMNPFASDYDLA
                     AIKGGEPDAMFDEAIKVFGGMSRFVKKGQTVVVKPNIGWDVEPERAGNTNPLLVNRII
                     KHCFDAGAKDVYVFDHTCDNWNKCYSNSGIERAVKDAKGKIVSGASESYYQEVTIKQG
                     KKLKKAKVHELILESDVFINVPILKHHSSADVTIGMKNLMGNVWDRGYWHSNNLHQCI
                     SDFTSFRKPDLTVVDAYFVMKKNGPRGVSKDDVIPLKSQIISTDIVAADAAAAKLFGV
                     DPASIDHIRIADEMKLGTMNLDKLSIYRKIM"
     gene            6752..8308
                     /locus_tag="VF870_11570"
     CDS             6752..8308
                     /locus_tag="VF870_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9252874.1"
                     /translation="MKLSYLRKIRIVVSIIFLLLIAIVFLDITNLLSVSLISTVTYLQ
                     FIPSALKFSNLFVPTSIGFIIVLVLTLLFGRVYCSSICPLGTIQDISSFTAKKINRKK
                     KFVYTKESVWLRYSVLAIVLISFLFGNLFLVNLLDPYSNTGRIFAQIFNPLLVSLNNL
                     AAFALEKFNIYLIYPIEMKSFYLVAALFPLIFLAIVIYLSYKRGRLYCNTICPVGTLF
                     GLISRFTIFKLKIDKASCEGCGVCARVCKAECIEKSTKEIDFSRCVACYNCLDVCPSD
                     SINYELITIKNNQSGSDESRRNFLSRTTLFFVGLTASAVSQIKIIPKKENKVEVKKKY
                     ISSPPGSKAIDHFNNNCTACHLCITACPTKVLQPSFTEYGIFNIFQPFMDYKTSFCNY
                     DCIACSEVCPTSAILPVTGDQKKNIQIGKAIFIKENCIVETENTACGACSEHCPTKAV
                     MMVDYKGNLKIPEVTNKYCVGCGACEYACPTKPYKAIYVDGNYIHQTAEKKPTEKIDQ
                     KIDYKEEFPF"
     gene            8563..>8974
                     /locus_tag="VF870_11575"
     CDS             8563..>8974
                     /locus_tag="VF870_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252875.1"
                     /translation="MKLHHLRIVLFLFFVVAIVGCKSEINSPDKANTDAAELLKFETV
                     ESDLALLKGGGMPLPEGDAVFSIGWNEIFRPFNDDSSIKGMAFAVAFGDRHTESPDFP
                     RFGIDMGTINIQYAGNQIEMHKMFHQRRGIAYSLF"
BASE COUNT         2984 a         1369 c         1665 g         2956 t
ORIGIN      
        1 taggcagaca acctattatc aatagtattg ctgggggtgt ttatgatggc gcatctctta
       61 cgcttaagaa aaccgattat aaatttaatg catattttgg cggtaacgtt ccagcttatc
      121 aaaaatttga gttgatagaa aattttggtg aaaattattt gttcggtgga aagtttacta
      181 ctacagtgct tcataatttt caactcggac ttggttatgt gaacaagaac tttcagcctc
      241 aaaattatac cgcagatcgt ttaaaggccg atcttactcc aattgaagtt gaaatacaga
      301 ataaatcaaa tcagtacgag tttgttacag gcgaagttaa ctacaatcag caaggattgg
      361 tttctatctt caccaggtat gattatgatc tgaatttcaa tcaaacatca aagtttgaat
      421 ttgatgcccg agcggatgca gttaaagatt gggcaataaa tctttatttt aattatcgtc
      481 agccaaaaat caggtataac tcaatatttg ctgtctttga ttatggaaat acttacgaag
      541 ttgaagttgg aactgattat aagatcaata gtatgttcac tgtaactggt aagttcggta
      601 atgtgcagta taaagatgaa aatgctcaaa gaattggtct tggtttagct acaacttacg
      661 gcagcataaa ctatagaaaa acttttggct atgccggaga attagacgca atttcattgt
      721 attccgggta tacattcttc gaaggattat ttacgccttc catagggctt tcctacacaa
      781 attataagct tagccctgat gatgaagcca ataatattac cactttaatg gttggtgcaa
      841 atatccgtcc tttccgtaca ctttcgtttg atctgcaggg acagtatatg gataacaaaa
      901 tttataagaa tgatatgaga ttattcttaa aacttaatta ctggtttaat ttaaacatct
      961 aatattctaa ctatgcaaaa attaaaagtt ttatatctgt ttgtcttttt gggatttgtt
     1021 atttttcttt tagtgggtgc gtttagtaca caaaaagatg ataaaccaaa aacgaacgag
     1081 ggaagaataa aattttccca ttcacttcat aatgaattaa ttgattgtca aacctgtcat
     1141 gcagcagtta gcgaaagtgt aagtttaaaa gatccgcttt tcccaaacca tgatgtttgt
     1201 tcatcatgtc atgaagtgga taatgaagac gagtgctcaa cttgtcatta tgatgataat
     1261 tatgaagcac ttgttaaaag tgaaaaagga ttaatattca atcacaaatt gcacttagct
     1321 gataaaggaa ccaattgtga atcctgtcat aaaggattaa gtgaagttga ttatagctgg
     1381 caatcagcag atgctcatcc accgatggaa acttgttatt catgccacaa tgataaaact
     1441 gttgcttcaa atgcttgcga aggatgtcac atttcaacag taaacttgtt acctcaggat
     1501 cataaagttg ttagttttgc aaagagccat aaatttgctg cccaggagtt taatgcaaat
     1561 tgcgtaatgt gtcacgataa tcaaagttgt gaagattgcc acgttgcaac aattggaatt
     1621 actgaaacca atactctcaa taacttctat cagccttatt atccaagtaa ttttgttgat
     1681 ggtgcaaaag ttcaggcaat aaatcgtgta cacgaactta attatagatt ttctcacgga
     1741 attgattctc gcggtaaaac cgctgaatgt caaacgtgcc atcagattga aacattctgt
     1801 gctgaatgtc atcaggcaga agaccaggat tttgcctttg gaggaattgt tcctgcgtcc
     1861 catttgaaat ctagttttaa aacaatcggt gttggttctg gcggtggtga tcatgcaatc
     1921 ctggctaaga gggacataga aagttgtatg gcttgccatg atgttaacgg tgctgatcca
     1981 acttgtataa cttgtcatct tgatagtgat ggaatcaaag gaacgaatcc caaaacacat
     2041 gctgcaggtt ttatgagaga tgaacacggt gattggcata atgataacag ttctatttgt
     2101 tataattgtc acacaagtgc atcaccgcaa tcgcagcctg gattaggctt ctgtggctac
     2161 tgtcatggaa caaatgtaaa tgattaatac tgaaaatatt ttacggataa actaatgaag
     2221 tatctaattt tatttataac acttttttta agctcagtgc tatttataag ctgcagcgac
     2281 ttaaaaaatg atatcccgct tactccagat ataaatattc atggaagtga agtttttgat
     2341 tcaactgctt ccaactttca tggcaaacag gtactcgata gtcagaatag ttttcaagat
     2401 tgtaaacagt gccatgatgc taattatagt ggtggaatag caaaagttag ctgctattct
     2461 tcagattgtc atgtttctcc ggcaataaat gtgcatgaag ctggtattac tgatgttcag
     2521 tctccgaatt ttcatggaaa atttattgct gataaagtta acggatcaat gatttcttgt
     2581 gcacaatgcc acggcgattc ctatcaaggc ggggtagttt caccttcttg tgcaaattgc
     2641 cattctggaa tccctgttca cgtgggtgat tatgttaatc caatctctcc gaattttcac
     2701 ggaaaattta ttgctgataa agttaacgga tcaatggttt cgtgtgcaca atgccacggc
     2761 gattcttatc aaggcggaat agcttcacct tcttgtgcaa attgccattc tggaattcct
     2821 gttcacgttg gtgattttgt taacccaagc tctccaaatt ttcacggaaa atttattgct
     2881 gataatttta gcggctccat gaattcttgt gcacaatgtc atggcgattc atttcaaggt
     2941 ggagttgcgt cgcctacttg cacaaattgt cattcaacta ttcctgtgca cgtggatggt
     3001 attgtaaatc catcttctcc aaattttcat ggtaagtata ttgtagcaaa tcttgcttgg
     3061 gatatgcgtg catgcggttc ctgtcactca tctgattatt caggaggaat tgccgcccca
     3121 agttgtttga cttgtcatac cagtacaaat ggacctgaag cgtgcaatac ttgtcatggt
     3181 gattttaatg atcctactaa aattgctcca cccagggcgc ttaatggaag tgcagtaact
     3241 acttacgccg gagtaggtgc tcatacatcg catcttatta caaataattt aggtagtgat
     3301 gttagttgtt caacttgtca tacttatccc caatcagttt acgcaaatgg gcatttaggt
     3361 agtgatggaa aagccgaaat acattttggc aacttagcaa cgcataatgg aattaatcca
     3421 acctatagct tttcaaataa tacttgttca gatacttatt gtcatggaaa ttttgctttc
     3481 tatagagatt catctaattt tgcttttgct tatactgctg caaccatgga aggcagtaat
     3541 ttctctccta aatggaatca agttgatggc agtcaggcag tgtgtggaag ttgtcatggt
     3601 ttaccgccag ttgggcatat tcctgctgaa cttagtactt gtgttaattg tcatccggga
     3661 gttgtcgatg gacagggcaa tatagttgat agaacaaagc acataaatgg tgttaaaaat
     3721 gtttttggaa attagtatta aaatctgata gaacttaaaa gccgcttttt gatagcggct
     3781 tttttatatt ttaaaatgaa aatttttaat aataataaaa caaaaaatga ggctaatggt
     3841 aatattagtc tatctgatta cgaacgtaaa ttagaagaac gtaaagaaga atatgaacat
     3901 ttgaatgaga ttttgcgagg gatgcgtgca gaagtgtcaa atctcatgga agaaattaaa
     3961 aatgttactg ataatcttgt aaagaaaaat gctgagttat ttttattaga gcaaagtatt
     4021 gatgacttaa aaagaagtta cgaactttta caacaaaatg taaaatatga ggaagaagca
     4081 tttagtaaag ttaacaatga gttaacaaaa attaaaaatg aaattactga aagccttccg
     4141 tatcacaata aaataatttc tgcgaaaaat gaatttgaaa tcttgtcaaa acaattaatt
     4201 gaatccaaag cagaaataga aaagacaaat aaccaaacca aactgttagg ggaaatttct
     4261 gaaaacgttg aacttaaaaa tgagttagat attccaattg ctacaaaaaa gcaaaagtgt
     4321 catgcaaaaa ctaaaaccgg atctaagtgt aaaaggtacg cggttgaagg cagtttatac
     4381 tgtgcttctc actcttcaat tgtcaatgaa ccaaattaaa atatccctaa ttaaatcata
     4441 tataattgat tctaatttta taaaaacttc agatagaatt aaaaaaaaat gcactttttg
     4501 aaaaatctta gagagccaat gaatggcttt acacacttta ttgggataat acttgccatt
     4561 atcgcaacta ttttgctgat aaatttatct ttaaatcctt atagaccaat gcatctaatt
     4621 tcatttacag tttttggatt tgggatgata ttgctttaca caataagtac tctttaccac
     4681 tggctaaaac tttctgatgc gggaatattg aaattgagaa aagctgatca cataatgatc
     4741 tttatttata tagccgctac atatactcct atttgcataa tagctttaaa gggaacactc
     4801 ggatggggat tattcggtgc agtttggttt gttgctttgg gtggaataat aattaaaata
     4861 ttttggatga aagctccaag gtggatttca accctcattt atattttaat gggatggtta
     4921 gctgtaattg taatttatcc tattgttaat tctttaccaa cagaggcact gatttggtta
     4981 ttgattggag gtttgttcta tactatcggt gcggtaattt atgctgtcaa aaagcctgac
     5041 ccttttccag gagttttagg cttccacgaa atatttcatg tttttgtttt attgggcact
     5101 ttctcacact tctggatgat gtataagtac attgcggtta tgaactgagt taatcagcca
     5161 ttcagctgtg attatttaat tgatttctat taatcaagta aatatatgta cttaaaacaa
     5221 ttgctgagat taattgaaaa taaaatggtg tcgaaaaaag tttgtcatct aaaagtgatg
     5281 aagatatcaa agtaaatttt aagaaatagt aaataacttt actgattata atacttacaa
     5341 agagtgatac aaaaacattt tttagcctat tttttaataa gaaaaacaaa ctaagattta
     5401 cgataagctc agtacttatg agaaatgtct ttaatatttg cggatgagat gaaatgaaaa
     5461 aggaaaataa aggtaatgta agagcaatta tataagcatt tttctttgaa gtgtgaatca
     5521 atgcaactac tagagctata cgcattggat caaaataata aacagggaat gcaaacaaat
     5581 gcgataatgc aggtaagaag taaatgatta acagcagaga aaaatcaatc aaataggttt
     5641 ttgcatttga tttgaaatcg attacactta tagctttttc catttatcat cctttatgaa
     5701 taattactga acaaaaataa accaaaagaa ttcaagctca aagaatatat tttagtctat
     5761 aaattataat ctaaattgat tattttacac aagttatttt aacatcattt tgcgagaggt
     5821 gttatgagaa gacgtgattt tatttccaaa tcttttaaag ccagtttaat tgccggggca
     5881 gggctttcag ttggcagcta ttctaattta tttccatctg caatgaatcc ttttgcaagc
     5941 gattatgatt tagccgcaat aaaaggcggt gaaccagatg ctatgtttga tgaggcaatc
     6001 aaagtatttg gcggtatgtc taggtttgta aaaaaaggac agactgttgt tgtaaaacca
     6061 aatataggct gggatgttga accggaaagg gcagggaaca caaatccgct tttggtgaac
     6121 agaattatta aacattgctt tgatgcgggt gctaaagatg tttatgtgtt tgatcatacc
     6181 tgcgataatt ggaacaaatg ttattcaaat agtggaatcg aaagggcagt taaagatgca
     6241 aaaggtaaga tagtctctgg tgcaagtgaa agttattatc aggaagttac gattaagcag
     6301 ggaaagaaat taaaaaaagc gaaagtacac gagttaatac ttgaatctga tgtatttatt
     6361 aatgtcccga ttttaaaaca tcattcatct gcagatgtta caattggtat gaaaaattta
     6421 atgggtaatg tttgggatag gggatactgg cattcaaaca atctgcatca gtgtatttcg
     6481 gattttactt cattccgtaa acctgattta acagttgttg atgcatattt tgttatgaaa
     6541 aagaatggac ctcgcggagt atcaaaagat gatgtgatcc cattgaaatc acagataatt
     6601 tctacagata ttgttgctgc agatgccgcc gcggctaagt tatttggcgt tgatcctgca
     6661 agtattgatc atataaggat tgcagatgaa atgaaacttg gtacaatgaa tttagacaag
     6721 ctttcaatct acagaaaaat aatgtgatat attgaagctt tcgtatctta gaaaaataag
     6781 aatagttgtc tcaataatat ttttgttatt aattgcaatt gtatttcttg atattacgaa
     6841 tttactttct gtaagcttaa taagcacggt tacttactta cagtttattc cttcagcatt
     6901 aaaattttca aatctatttg ttccaacttc aattggcttt attatagtgc ttgttcttac
     6961 tttattattc ggacgagtat attgttcaag tatttgtcca ttaggaacta tccaggacat
     7021 atcctccttt acagctaaaa agattaatag aaagaaaaaa tttgtttaca caaaagaatc
     7081 tgtgtggctg cgctattcag ttctcgcgat cgttttaata tcatttctat tcggaaattt
     7141 atttttagta aatcttttag atccttatag taatacagga agaatattcg ctcagatatt
     7201 taatccattg ttagtgtccc ttaataattt agctgcattc gctcttgaga aatttaatat
     7261 ttatttgatt tatcctattg agatgaagtc attttactta gtcgctgcat tgtttccttt
     7321 aatcttcttg gcaattgtaa tatatctttc ttataagagg ggaagattat actgtaatac
     7381 tatttgccca gttggtactc tgttcggact catttcaaga tttacaatct ttaaactgaa
     7441 aattgataaa gcaagctgtg aaggctgtgg tgtatgtgcc agagtatgta aagctgagtg
     7501 tatcgaaaaa tcgactaaag aaattgattt cagccgttgt gttgcctgtt ataattgttt
     7561 ggatgtatgt ccaagtgatt caataaatta tgaattaatc acaatcaaaa ataatcagtc
     7621 tggttcggat gaatcaagaa gaaatttttt aagcagaacc acattattct ttgtgggact
     7681 tactgcaagt gcagtatcac aaattaaaat aattcctaaa aaagaaaata aagttgaagt
     7741 aaagaaaaaa tatatttctt caccaccggg ttcgaaggcg attgatcatt ttaataataa
     7801 ctgtaccgca tgtcatctgt gtataactgc ctgtcctaca aaagtcttac agccttcttt
     7861 tactgaatat ggaattttca acatcttcca accatttatg gattataaaa ctagtttttg
     7921 taattatgat tgtattgcgt gcagtgaggt ttgtccgact tcagcaattc ttcctgtaac
     7981 cggagatcag aaaaaaaata ttcagatcgg aaaagcaata tttataaaag aaaattgtat
     8041 tgtagaaact gaaaacactg cgtgcggtgc atgctcagaa cattgcccga ccaaagcagt
     8101 tatgatggtt gattataaag ggaatctaaa aattccggaa gtgacaaata aatattgtgt
     8161 cggatgcgga gcgtgcgaat atgcttgtcc cacaaaaccg tataaagcta tttatgttga
     8221 tggaaactat attcatcaaa cagcagagaa aaagcccacc gaaaaaattg atcagaaaat
     8281 tgactataaa gaggaatttc cattctaaca aatatagccc tccgaaacat tcagatgata
     8341 attatattgg cagtggaaat tcaatatata tgaaataggc tccagctttc gcaagaataa
     8401 taatcgaacc gaaatttatt ttttacaaac ctcagatatt tagtggatta aaatataccc
     8461 ttatttcttt aaacccttcc aaatatattt ttgtctaaaa gttgaattac aggatttcta
     8521 ttaataattg tttaatttta ctcataatat ttaggagaaa agatgaaact tcatcattta
     8581 aggattgtct tatttttatt tttcgttgtt gcgattgtcg gatgtaagtc cgaaataaac
     8641 tcaccggata aggcaaatac tgatgcggcg gagttattaa aatttgaaac cgtcgaaagt
     8701 gatcttgcat tattaaaagg aggcggaatg cctttaccgg agggagatgc agttttttct
     8761 atcggctgga atgagatttt cagaccattt aatgatgatt caagtataaa agggatggca
     8821 tttgcagttg cctttggtga tcgacacact gaatctcctg actttccaag atttggtata
     8881 gatatgggta ctataaatat tcagtatgct ggaaatcaaa tcgaaatgca taaaatgttt
     8941 catcaaagaa gaggcatagc atattcatta ttcc
//
LOCUS       DASWBY010000636         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_176548, whole genome shotgun sequence.
ACCESSION   DASWBY010000636 DASWBY010000000
VERSION     DASWBY010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176548"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VF870_11580"
     CDS             complement(<1..533)
                     /locus_tag="VF870_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX9252876.1"
                     /translation="MSKTKIKYICSNCGYESLRWIGKCPECESWNTFTEEIVETSPRK
                     ANISKSRFSYSKILEISANEDDRIKTGIIEFDRVLGGGIMPGSVILLGGDPGIGKSTI
                     AMQAAASIKEKVLYVTGEESEKQIKLRSGRLKVKSDLFFVQAETELSNILGAIKEISP
                     SVVIIDSIQTTYRSELE"
     gene            complement(757..>1664)
                     /locus_tag="VF870_11585"
     CDS             complement(757..>1664)
                     /locus_tag="VF870_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX9252877.1"
                     /translation="THFLHKALRAVLGTHVQQAGSYVGPDRLRFDFTHFAKLTADEIQ
                     KIESIVNEQTRKNYPLNHHRNTPFDEAKKMGALMFFGDKYGDKVNVVQFGDFTMEFCG
                     GTHVHNSSEIGLFKILNESSIASGVRRIEAVTGAGVEKYIQTQTEQLKLSEQKIEELL
                     DVKKKLEKEVAELRMKEKLEQLDHILSLSSDEKNITIFKGKVHADSMDELKSFGDEMR
                     NKIKSGVGVLIAQIEDKVGIVAIVSDDLIKDKKLSAGKIVGELAKLVGGGGGGRPHLA
                     TAGGKDVNQIVTALSKVEKIVGSFL"
BASE COUNT          492 a          348 c          250 g          574 t
ORIGIN      
        1 ttttctaatt cacttctgta ggttgtttgg atagaatcta taatcacaac agaaggcgag
       61 atttccttaa ttgctccaag tatatttgat aactctgttt cagcctgaac aaaaaataaa
      121 tctgatttta cttttaatct gccggagcgg agtttaattt gtttttcaga ttcttcgcct
      181 gttacatata aaactttttc tttaatgctt gcggcagcct gcattgcaat tgttgattta
      241 ccaattccgg gatcgccgcc aagcagaata accgaaccgg gcattattcc accgccaagc
      301 acacgatcaa attctataat gcctgttttt attctgtcat cttcattagc agaaatttcc
      361 aaaatcttgc tgtagctaaa tcttgatttt gagatatttg ctttgcgagg ggaagtttct
      421 acaatttctt ctgtaaaggt attccaactc tcgcactccg ggcatttgcc tatccagcgt
      481 aaagattcat atccgcagtt agaacagatg tattttattt ttgttttact cataatatgt
      541 caatggtcaa aagcgaaatg tcaaaaaaaa tattaaaaca gcattaatct ttggaaacag
      601 tattaaaaat ttattccgtt atttcgaatc ctcatttaga gcgtgaagca atctgaacaa
      661 ctttttgagc tgctttgttt tgagattgct tcgttcgaag actcgctcgc aaagactata
      721 aaagaattgc ttaacaaaaa acatagttaa attaaactac aaaaagcttc caacaatttt
      781 ttcaacttta gaaagagctg ttacaatttg attaacatcc ttaccaccgg cagttgcaag
      841 atgcggccga ccgccgccgc cgccgccaac taattttgca agctcaccga caatttttcc
      901 ggcactaagt tttttatctt taattaaatc atcacttaca attgctacaa tcccaacttt
      961 atcttctatt tgagctatca acacaccaac accacttttt attttattgc gcatctcatc
     1021 accaaaggat ttaagctcat ccatagaatc agcatgaacc ttacccttga atatcgttat
     1081 gtttttttcg tcagaagata atgacaaaat atgatcaagc tgttcaagtt tttctttcat
     1141 tcttaactca gcaacttctt tctcgagttt tttcttcaca tcaagaagtt cttcaatctt
     1201 ttgttcagat aatttaagct gctccgtttg tgtttgaata tatttttcaa cgcccgcacc
     1261 cgtaaccgct tcaattcttc tgactccgct tgcaatagat gattcattca atattttaaa
     1321 caatccgatc tctgatgaat tgtgaacgtg tgtgccaccg caaaactcca tagtaaaatc
     1381 accgaactgt accacgttaa ctttatcacc atacttatcg ccgaaaaaca tcagtgcgcc
     1441 catctttttt gcttcatcaa acggtgtatt gcgatgatgg tttaatggat aattttttct
     1501 tgtttgttca tttacaattg attcaatttt ttgaatttca tcagcagtaa gttttgcgaa
     1561 atgagtaaag tcaaatctta atctatccgg tccaacataa gaacctgctt gctgaacgtg
     1621 agttcccaaa acagctctta atgctttatg caaaaagtga gtta
//
LOCUS       DASWBY010000637         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_705312, whole genome shotgun sequence.
ACCESSION   DASWBY010000637 DASWBY010000000
VERSION     DASWBY010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705312"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VF870_11590"
     CDS             <1..391
                     /locus_tag="VF870_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9252878.1"
                     /translation="DVNTINTNNEQRDTHLKSADFFDAENHPKMIFESKSIKKTSDSE
                     MQVTGNLTLRGVTKEITLDVIYNGTVSGFGDSTITGFEVRGKINRFDYGLKWNALTEA
                     GGVVVSNEVKIEILAEFNKVNEAIKAA"
     gene            544..1098
                     /locus_tag="VF870_11595"
     CDS             544..1098
                     /locus_tag="VF870_11595"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9252879.1"
                     /translation="MAFKNKIILNPKTRNEIKFLQTSKDSDGKLLEMESTYFENSKEP
                     PPHYHPYQEEDFTVLSGEISIRINGRINILKQGESIHIPANTVHSMWNNSDNKTIVNW
                     RVRPAMETEFMLETLIGLAKDGKTNEAGVPGLLQISLTANKFSNVLRLDNPPFILQKI
                     IFTLLSPLAIALGCRAMYSKYIDR"
     gene            1287..2282
                     /locus_tag="VF870_11600"
     CDS             1287..2282
                     /locus_tag="VF870_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9252880.1"
                     /translation="MQKRMLGKSGLEVSAIGLGCMGMTFAFPPFPEKKEMISLIRTAV
                     ERGVTFFDTAEVYGPYSNEKLVGEALEPLRDKVVIATKFGFELDPNGGPKWTGFNSRP
                     EHIRAVAEASLKRLKTDVIDLFYQHRVDPKVPIEDVAGTVKDLITEGKVKHFGLSEAG
                     VQTIRQAHAVQPVTALQSEYSLWWREPEKEIFPLLEELRIGFVPFSPLGRGFLTGKIN
                     ENTKFESTDFRSTLPRFAPEARKANQVLVDLLTRVAKQKGATPAQLALAWILAQKPWI
                     APIPGTTKLNRLDENIAAASIELTESDLRELESAAPKIKIEGERYPQNLQQAVNR"
     gene            complement(2442..>2777)
                     /locus_tag="VF870_11605"
     CDS             complement(2442..>2777)
                     /locus_tag="VF870_11605"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9252881.1"
                     /translation="EPGSATRTVMENYFAKIKKRKSIELTSNEAVKQAVMAGIGYSIL
                     PLIGIKNELLKKQLFIIKAKGLPVTTKWRLIRLENKKLSPVAKAYLDYLSVNKEKIII
                     NNFKWYLDF"
BASE COUNT          957 a          469 c          500 g          851 t
ORIGIN      
        1 tgatgtaaac accatcaaca caaataacga acagcgtgat actcacttaa aatctgctga
       61 tttctttgat gcagaaaatc acccaaagat gatttttgaa tcaaaatcta ttaagaaaac
      121 atctgattct gaaatgcagg tgacaggaaa tctgacactt cgcggagtaa ctaaagaaat
      181 tacgcttgat gtaatctaca atggaactgt ttcgggattt ggagatagta caatcacggg
      241 atttgaagta cgcggaaaga taaaccgttt tgattatggt cttaagtgga atgcattaac
      301 cgaagctggt ggagtagtag taagcaatga agtaaaaatt gaaattcttg ctgagttcaa
      361 caaagtaaac gaagctatta aagcagcata atattattga actttttatt aattgaaaac
      421 tcaagggcac ctggtgccct aattttttat tcctactatt gtttcctttg gaaagaatta
      481 taagatttta tctacctaaa attttaagat aaatatcatt ttaaaattta accggataat
      541 ataatggcat ttaaaaacaa aataatatta aatcctaaaa ccaggaatga aataaagttt
      601 ttacagacca gtaaagacag cgacggtaaa cttttagaaa tggaatcgac ttattttgaa
      661 aactcaaaag aaccgccacc acattatcat ccttaccaag aagaagactt tacagtttta
      721 tccggagaaa tttcaataag gataaatgga agaataaata ttcttaagca gggggaatcg
      781 attcacattc cagctaacac tgttcactca atgtggaata actcagacaa taaaacaatt
      841 gttaactgga gggttcgtcc agccatggaa acagaattca tgctagaaac tttgattggg
      901 cttgcgaaag acggaaaaac aaatgaagca ggggttccgg gattattaca aatttcatta
      961 acagctaata agttttcaaa tgtactccgg ttagataatc caccattcat tcttcaaaaa
     1021 attattttta ctctgctttc cccactggca atagcattag gatgtagagc catgtattca
     1081 aaatatattg atagataacc tttaagctgt ccaataaaat ataaaaccgc agaaaatact
     1141 ttcttcagtt ttttgatttt aagaatcaga catgatcttt taacaatatt gacctatgtt
     1201 acaccaatac aatttctgtt aatatatttt tgatctataa atttagatag ttcaatattt
     1261 tcttatcaaa acaaagagga ttaaaaatgc aaaaacgtat gcttggaaaa agtggattag
     1321 aagtatctgc aatcggactc ggctgtatgg gaatgacatt cgcctttcct ccgtttccgg
     1381 aaaagaaaga aatgatttct ttaatccgta ctgctgttga gcgcggtgtt acatttttcg
     1441 atactgcgga agtttatggt ccatattcaa atgaaaagct tgttggcgaa gctcttgaac
     1501 ctttacgtga taaagttgtt attgcgacaa agtttggatt tgaacttgat ccgaacggcg
     1561 gacctaaatg gactggtttt aacagcaggc cggaacacat tcgagctgtt gcagaggctt
     1621 cattaaagag attgaaaaca gatgttattg atttatttta tcagcatcgt gttgatccga
     1681 aagtgccgat agaagatgtt gcaggaactg taaaggattt aattacggaa ggcaaagtaa
     1741 aacattttgg tctttctgaa gctggagtac aaacaattag acaagctcac gcagttcaac
     1801 ccgttaccgc attgcaaagc gaatactcac tgtggtggag agaacctgaa aaggaaattt
     1861 ttcctttact tgaagaactg agaatcgggt tcgttccgtt cagtccatta ggaagaggat
     1921 ttcttaccgg aaaaataaat gagaacacaa agtttgaaag cactgatttc cgaagcactc
     1981 ttccaagatt tgcacccgaa gcacgcaagg caaatcaggt attagttgat ttattaacta
     2041 gggttgctaa acagaaaggt gcaacacctg cacaacttgc acttgcctgg atacttgcac
     2101 agaaaccatg gatcgctcca attcccggaa caacaaagct aaacagatta gatgaaaata
     2161 ttgccgcagc atcaatagaa ttaacagaaa gtgatcttcg tgaacttgaa agtgctgcac
     2221 ccaagataaa aattgaaggt gagcgttatc ctcaaaacct tcagcaggca gttaatcgat
     2281 agcttttatt cagtcaataa tttgtcatac tgagcgaagt cgaagtataa cacaatgctc
     2341 atttttagcc gcagaaaaag tttctgcggt tttttaataa ttttacttat taaagacctg
     2401 tgttatgtaa atgatatttt tttattatcc taatttctaa atcaaaaatc caaataccat
     2461 ttaaaattat taataataat tttttctttg ttgacggaga gataatctag ataagccttt
     2521 gcaaccggag ataacttttt gttttctaac cgaattaatc tccattttgt tgtgacgggt
     2581 aatcctttag ctttgattat aaaaagttgc ttttttaata gttcattttt aataccaatc
     2641 agtggtaaaa ttgaataccc aattccagcc ataacagcct gtttaactgc ttcattcgaa
     2701 gtgagctcga tactttttct ctttttgatt ttagcaaaat aattttccat cacggttctt
     2761 gtagcggaac ctggttc
//
LOCUS       DASWBY010000638         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_480067, whole genome shotgun sequence.
ACCESSION   DASWBY010000638 DASWBY010000000
VERSION     DASWBY010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480067"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VF870_11610"
     CDS             complement(<1..780)
                     /locus_tag="VF870_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252882.1"
                     /translation="MAYSTRGILYTAPKLIYTLLFILILFQDKTPAQLYQYDTENLRL
                     INNGEASKYLVKYVGQCFENALKFHRELYNYDSDEITTLLVYDLNDFGNAGAGTIPRN
                     HIVLSIAPLNYEYETSPANERFNTTFNHELVHLVTLDQSTSTDRFYRTIFGGKVGESS
                     DDPLTIIYAYLTDPRRSTPRWWKEGIAVFLETWMAGGIGRAMGGYDEMVFRTLVQEEK
                     PIYDIISLETEGTQTDFQVGANSYLYGTRFMSYLSLKYGPEK"
     gene            complement(869..1777)
                     /locus_tag="VF870_11615"
     CDS             complement(869..1777)
                     /locus_tag="VF870_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252883.1"
                     /translation="MTEKSSKRNSTKFRAIVAGSTIILVAVIYFAIVYPWPNENVFGT
                     VGGVEKAKKYQKEQITKNDVALQNSEIHDLIQNDQIQKLINDPNFRTAVENESFRTEF
                     NRNQVFRNAILSDAYRTTMSNRNWVEALRNESFRSIVESGAFAKIVSDGSLTEALRNE
                     SLRSALAGANWRAAVESNRALSNLMSDASFKTAMSDAAFRQLVAGDGMRQLLNNRQFT
                     EAFRTEASRNIYQDASFQEALRQASFREAASSESFMRTLSNETFRTEAFRSEAFRTEV
                     FRSEAMRNEALRNETNRNIESSRINN"
     gene            complement(1891..2664)
                     /locus_tag="VF870_11620"
     CDS             complement(1891..2664)
                     /locus_tag="VF870_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252884.1"
                     /translation="MIDKTMIELINKDIDKTISPGEKEKLSQYLKENPDANVLYNELL
                     KAEHMLDKLEDLIPSPNLKERILNSIDYNLYSTKKKNSEVLDYLSSIFAGSRSKFAVS
                     FALGMIAGLIILTVIFYNSTFHDELNNYNTSGTIGLHKSNIIQTIDILSEDIYGSVDV
                     SKVNNFYGFDIKLNSQDKYRLLIEFDSANLSLDNFSLNDLNSVSLEQGPDYVQISGSQ
                     DLDYFLSFSAKASTSEKFILKILKENREVFKREVFVSKN"
     gene            complement(2666..>2849)
                     /locus_tag="VF870_11625"
     CDS             complement(2666..>2849)
                     /locus_tag="VF870_11625"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX9252885.1"
                     /translation="TDALMELDMLYRMPVVLKHFLDYSYKELSYLLSVPEKTVKSRLF
                     TGRQLLKDILVKKRVL"
BASE COUNT          908 a          503 c          400 g         1038 t
ORIGIN      
        1 tttctctggg ccatatttaa gagaaagata actcataaat cgcgttccgt acaaatacga
       61 atttgcacca acctggaaat cggtttgagt tccttcggtt tcaagactta taatatcata
      121 aattggtttt tcttcttgaa caagtgttct aaaaaccatc tcatcatatc ctcccattgc
      181 tcttccaatt ccacctgcca tccatgtttc aaggaataca gcaatacctt ccttccacca
      241 gcggggcgtg cttcttcgtg gatccgtgag ataggcataa attattgtta acggatcatc
      301 tgaagattca ccaactttac caccgaatat tgtccggtaa aatctgtcag tggaggttga
      361 ttgatctagg gttacgagat gtaccaattc atgattaaat gtagtattaa atctttcatt
      421 tgctggagaa gtttcatact cgtaattaag aggggcaata gatagaacta tatgattacg
      481 cggaattgta cctgctccgg catttccaaa atcatttaaa tcgtatacaa gtaatgttgt
      541 tatttcatcg gaatcataat tatatagttc tctatgaaat tttaatgcat tttcaaaaca
      601 ttgtccaaca tatttaacaa gatatttact tgcttctcca ttatttatca atctcaaatt
      661 ttcagtatcg tactgatata attgtgcagg tgttttatcc tgaaataata taaggataaa
      721 taaaagagta taaattaatt ttggggcagt gtataaaatt cccctggtac tgtatgccat
      781 aaattagttt tatttaaaat ataataagga gaaactatct aaccaataat ttctcctcac
      841 aaattaataa taatatttat ttcaaatatt agttgtttat tctgcttgac tcaatatttc
      901 tgtttgtttc atttcttaat gcttcgtttc tcatcgcttc agatctgaat acttcagttc
      961 tgaatgcttc gcttctaaat gcttcagttc tgaatgtttc attcgataag gttctcataa
     1021 agctctcgct tgaagctgct tctctgaatg aagcttgtct aagagcttcc tggaaggatg
     1081 catcttgata tatgtttcta ctggcttcag ttctaaatgc ttctgtgaac tggcgattat
     1141 ttaataattg tctcattcca tcgccagcaa caagctgtct aaatgcagca tcagacatag
     1201 ctgttttgaa ggaagcatct gacattaaat tacttaaagc acgattggat tcaactgctg
     1261 ctctccagtt tgcaccagct aatgcagatc taagtgattc gtttcttaat gcctctgtca
     1321 atgaaccatc agaaactatc tttgcaaaag caccgctttc aactatactt ctaaaacttt
     1381 catttcttag agcttctacc caatttctgt ttgacatagt agttctatat gcatcactga
     1441 ggattgcatt tctaaagact tgatttctgt taaattcagt tctaaatgat tcattttcca
     1501 ccgctgtcct aaagttagga tcattaataa gtttctgaat ttgatcattc tgaattaagt
     1561 catgaatttc agagttctga agagcaacat catttttagt gatttgttct ttttgatatt
     1621 ttttagcttt ttccactcct ccaactgtac caaagacatt ttcattcggc catggatata
     1681 caattgcaaa atatataact gcaactagta taatagttga ccctgcgact atagcacgaa
     1741 attttgtact gtttcgtttg ctgcttttct ctgtcatgtt aacctctctt tttagttgtt
     1801 ttaaaaaaac ctccgaatca attattacaa atacaatttg gacgaagaaa tgttggtgga
     1861 attcacattt ttattgaaat aatagaaaaa tcaatttttt gaaacaaaaa cttctctttt
     1921 aaatacctct ctattctctt taagaatctt taatataaat ttttctgaag ttgaagcttt
     1981 tgctgaaaat gaaaggaaat aatctaaatc ctgagaacca gaaatttgga cataatcagg
     2041 cccttgctcc aaagaaaccg aattcagatc atttaacgaa aaattatcca aagaaagatt
     2101 agctgaatca aattctatta ataatctata tttatcctgt gaattaagtt taatatcaaa
     2161 accataaaaa ttatttacct tgctcacatc aacactacca taaatatctt ctgataaaat
     2221 atcaattgtc tgaattatgt tggatttatg aaggcctatt gtacctgatg tgttatagtt
     2281 gttcaattca tcatgaaatg ttgaattata aaatataaca gtgagaataa ttaaaccggc
     2341 aatcattcca agtgcaaatg aaacagcaaa cttggatctt gaaccagcaa atattgatga
     2401 taaataatct aaaacttctg aatttttctt cttggttgaa tacaaattat aatcaattga
     2461 attcaatatt ctttctttca aattaggaga aggaattaaa tcctccagtt tatccagcat
     2521 atgttctgct tttaataatt cattataaag aacatttgca tcagggttct ctttcaaata
     2581 ctgacttaat ttttccttct caccaggtga aattgtcttg tcaatatctt tattaattaa
     2641 ctcaatcata gttttatcta tcattttata atactctttt ctttactaat atatctttta
     2701 gcaattgtct tcccgtaaaa agtcttgact ttactgtttt ttcaggtaca cttaataaat
     2761 agcttaattc tttataagaa taatcaagaa aatgtttcag aactacaggc attctatata
     2821 gcatatcaag ttccattaaa gcatctgtg
//
LOCUS       DASWBY010000639         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_212255, whole genome shotgun sequence.
ACCESSION   DASWBY010000639 DASWBY010000000
VERSION     DASWBY010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212255"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF870_11630"
     CDS             <1..409
                     /locus_tag="VF870_11630"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9252886.1"
                     /translation="FPGNTEEAFIFYKKVFGGDFLGGIFRFKDTPDSDKISEGDRQKV
                     MHVALPLGPNNMIMATDALESMGFKVTFGNNIYISIHTDSREEADKLFNGLSEGGKIE
                     MPMADQFWGDYFGSLTDKFGVQWMVIYSAPKEN"
     gene            431..982
                     /locus_tag="VF870_11635"
     CDS             431..982
                     /locus_tag="VF870_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX9252887.1"
                     /translation="MRKLIFAINITIDGFADHTAGIADDELHGFFTNFLNTIDVTLMG
                     RKTYQLMENYWPNAHKDPNATKSIIDFADKFNTMKKIIFSNTLKEVKWKNSSLASESL
                     LKVVSDLKNQNGKNISAGSLSIANQLLNENLIDEFWFLIHPVVLGKGKKLFDGLEMKS
                     QLHLVDIRKLNSGVVVLHYHNLK"
     gene            1016..>1592
                     /locus_tag="VF870_11640"
     CDS             1016..>1592
                     /locus_tag="VF870_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9252888.1"
                     /translation="MQKITPHLWYDREAKEAAEFYCSLFPDSKITNITTLHNTPSGDA
                     DIVSFELYGQKFMAISAGPFFKLNESISMFAYCESDEGIEKVYNKLSEGGKVVFPLDK
                     YDWSPKYAWVVDKFGLSWQLDVEKINHQQKILPALLFVNDKVLKVKEAAEFYCSIFPN
                     SKLMMEFPYDKSAGLPDGSLLFAQFKLSDYLF"
BASE COUNT          535 a          238 c          307 g          512 t
ORIGIN      
        1 ttttccaggt aacacggaag aagcatttat cttttataaa aaagtttttg gtggagactt
       61 tttgggtggc atcttccgct ttaaagacac acccgattca gataaaataa gtgaaggcga
      121 tcgccaaaag gttatgcatg ttgctttacc tttgggtcca aataatatga ttatggcaac
      181 cgatgcacta gaatcaatgg ggttcaaagt aacgtttggt aataacattt atatctcgat
      241 tcacaccgat agcagagaag aagcagataa actatttaat ggactgtcgg aaggcggaaa
      301 aattgaaatg cctatggctg atcagttttg gggcgattat tttggatcac ttactgataa
      361 gtttggtgtt cagtggatgg taatctattc tgctccgaaa gaaaattaat attaagttaa
      421 agtcgttcca atgagaaaat taatctttgc aatcaacatt acgattgatg gttttgctga
      481 tcacactgca ggtattgccg atgatgaact gcatggcttt tttacaaatt ttttgaacac
      541 tatagacgtc acccttatgg gccggaagac ctatcagctt atggaaaatt attggcctaa
      601 tgcacataaa gatccaaacg caaccaaaag cataattgat tttgctgata agtttaatac
      661 aatgaaaaaa ataatttttt caaatacctt aaaagaagtt aaatggaaaa attccagttt
      721 agccagcgaa agtttgctga aagttgtttc cgatttgaag aatcaaaatg gaaaaaacat
      781 atccgccgga agcctgagca tcgctaacca attattaaat gaaaatttaa ttgatgaatt
      841 ctggtttcta atccatccgg tagttttagg aaaagggaaa aaattgttcg atggactgga
      901 aatgaaaagc caacttcatc tggttgatat caggaagctt aattcgggtg tggttgttct
      961 ccattaccat aacttgaagt gatttattta ttaaccaaaa taaaaggata ttataatgca
     1021 aaagataact ccccatttat ggtatgatag agaggctaaa gaagctgcag agttttattg
     1081 ttcacttttt ccggattcaa aaattacaaa cataacaacg cttcataata ctccttcagg
     1141 agatgctgat attgtttcat ttgaacttta tggacaaaaa tttatggcga taagtgcagg
     1201 accatttttc aaattaaatg aatcaatatc aatgtttgct tattgtgaat ctgatgaggg
     1261 aattgaaaaa gtatataaca agttatctga aggtggaaaa gttgtgttcc cactcgacaa
     1321 atatgattgg agccctaaat atgcatgggt tgttgataag tttggtttat cgtggcagct
     1381 tgatgttgag aaaataaatc atcagcagaa aattttgcct gcacttttat tcgttaacga
     1441 taaagtttta aaagttaaag aagctgctga attctattgc tcaatttttc caaattcaaa
     1501 attaatgatg gaatttcctt atgataaatc tgctggatta ccggatggat ctttactgtt
     1561 tgctcagttc aaattatcgg attatctttt ta
//
LOCUS       DASWBY010000640         3867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_550888, whole genome shotgun sequence.
ACCESSION   DASWBY010000640 DASWBY010000000
VERSION     DASWBY010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550888"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VF870_11645"
     CDS             <1..894
                     /locus_tag="VF870_11645"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX9252889.1"
                     /translation="FNFCRYNGDEIVYNAIPLQGKRKWICATRVPYGKTVEETFKQIF
                     SQLPDTDLLFDGCTNENRDFLKAYGFNSITFGKEAIIDLSKNEFQKKSLKELIKRGKR
                     HGAVVEVKYSEDNEKKLQEFFVQTSHGREPRLQNLFATAFKPYHRLFVLKKDEIWISA
                     IMLSFKDEKFMQTELILRSSKAPVGVMEALINEILIQLKKEELEYWSLGAVPFVDYDS
                     KFLSLSWFFNSIGRKIKFAYNYRGLFNFKNKFNPIWQPYYLCYKNKLNPIQIFQLSQK
                     SNLNKLIIYNILTKLGFNYGK"
     gene            896..2329
                     /locus_tag="VF870_11650"
     CDS             896..2329
                     /locus_tag="VF870_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX9252890.1"
                     /translation="MENLNDMPSEEFKKNGYKLVDWIADYLNNIEKYPPLSQVNPGDI
                     LKKLPNTPPTNGEDIENVLNDVDKILLEGITHWNHPGFMAYFNSTSSGPGILAELLTA
                     GLGVNGMLWKTSPAFTELEKSMMNWFRQMVGLPENYWGIIYDTASTSSMHAIASAREQ
                     LDLQIREKGMAGRNDISRLRLYCSEQAHNSIDKGALTLGIGLEGIRKIPVNDKFEMIP
                     QKLEDAIREDKLNGWLPLIVIATVGTTSTTSIDPVEAIAEITEKYKLWLHVDSAYAGV
                     TAMIPEMKWITRGWDRADSLVINPHKWMFTPMDLSVYFTRKPDILKRAFSLSAEYLKT
                     NQDSEVENLMDYGIQLGRRFRSLKLWFIIRYFGVNGLSERIKNHINLAKELESWIKNE
                     SDFELMAPVPFSTVCFRYHPANKDENELNEINENLLEKINASGKIFLSHTKLNGKFTI
                     RLTIGSIRHERRHIEEAWELIKSIARG"
     gene            2364..3002
                     /locus_tag="VF870_11655"
     CDS             2364..3002
                     /locus_tag="VF870_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX9252891.1"
                     /translation="MDSILQFSLLAFTSLFTMVNPLGVIPVFTTLTTGMTSKQAASIA
                     LKATSTALIVLLLFTFGGNFIFDIFNISVNGLRVVGGILFFLSGYDMLRGKLTRIKSD
                     EEKDDEAVKNFAIAPLGVPMITGPGVITISIVMMNDAPDVIHRSLLITSIFVVMGLTH
                     LILLSSRKITAFLGESGNKVLTRIMGLIVMVIAVELFFRGLKPIIQDIFLIK"
     gene            3078..3506
                     /locus_tag="VF870_11660"
     CDS             3078..3506
                     /locus_tag="VF870_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9252892.1"
                     /translation="MATKKALVKQVKGITFVGKTDSNHWITMDGPESFGGSDAGIRPK
                     ELLMLSLGGCTGSDVTSILGKKKIKLDGFEINITADSADEHPQVFTRMHLEYVFYGKN
                     LPIKDIERAIELSLTKYCSVTAMLQKAMPIEHSYKIVEVE"
     gene            complement(3569..>3867)
                     /locus_tag="VF870_11665"
     CDS             complement(3569..>3867)
                     /locus_tag="VF870_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9252893.1"
                     /translation="SKDFYHKVLGWEFKPFGNGYLLFNNHKGTMVGLRKVDEVAKGEA
                     TVFHVNMPDIDSILKKVKENGGAVKRTKTVIPAMGWYALFNDPDGNTLGLYQKS"
BASE COUNT         1362 a          579 c          697 g         1229 t
ORIGIN      
        1 ttcaattttt gcagatacaa tggagatgaa atagtatata acgcaattcc gcttcaagga
       61 aaaagaaaat ggatttgtgc aacaagagtt ccgtatggta aaactgtcga agaaactttt
      121 aagcagattt tttcacaatt acctgataca gatttacttt ttgatggatg cacaaatgag
      181 aacagagatt ttttaaaggc ttatggcttc aattcaatta catttggtaa agaagctatt
      241 attgatttat caaaaaatga atttcaaaaa aaatcactaa aagaacttat taaacgtgga
      301 aaaagacatg gagccgttgt tgaggtaaaa tattctgaag acaatgaaaa aaaattgcag
      361 gagttttttg ttcagacctc gcatggaagg gaaccacgac tacaaaatct ttttgcaacc
      421 gcttttaaac cttatcacag attattcgtt ctcaaaaaag atgaaatttg gattagtgca
      481 attatgcttt cattcaagga cgaaaaattt atgcagacag aattaattct taggagctca
      541 aaagctcctg taggtgttat ggaagcattg attaatgaaa tattaatcca gctgaaaaag
      601 gaagaacttg aatactggtc tttaggtgcg gtaccctttg ttgattacga ttcaaaattt
      661 ttatcactct cctggttttt taattccata ggaagaaaaa ttaaatttgc atataactat
      721 aggggtctct ttaattttaa aaataaattt aatccaatct ggcagccata ttatctctgt
      781 tataagaata aattaaatcc aattcagatt tttcaacttt cacaaaaatc aaatcttaac
      841 aaactaatta tatacaatat attaactaaa ctgggcttta attatgggaa atgatatgga
      901 aaacttaaat gatatgcctt ctgaagaatt caaaaaaaac ggatacaaac ttgttgattg
      961 gattgctgat tatttaaata atattgaaaa atatccaccg ctttcgcaag taaatcctgg
     1021 tgatatatta aaaaagcttc caaatactcc accaacaaat ggagaagata ttgaaaatgt
     1081 tttgaacgat gttgataaaa ttcttttaga agggattaca cattggaatc atccgggttt
     1141 tatggcgtac ttcaactcaa catccagcgg accgggaatt ttagctgaac tactaaccgc
     1201 aggtttaggt gttaacggta tgctctggaa aacttctccc gcatttacgg aactagaaaa
     1261 atcaatgatg aattggttca ggcaaatggt tggattacca gaaaattatt ggggaattat
     1321 ttacgatact gcttcaacta gctctatgca tgcaattgct tctgcgcgtg agcaacttga
     1381 tcttcaaatt cgtgaaaaag gaatggcagg cagaaatgat atttcgagat taagattata
     1441 ttgttcagaa caggcgcaca attcaatcga taaaggtgca cttactcttg gaattggatt
     1501 ggaaggaata agaaaaattc ctgttaatga taagtttgaa atgataccgc aaaaacttga
     1561 agatgcaatt agagaagata aactaaatgg atggcttccc ttaattgtta ttgcaactgt
     1621 cggaacaaca tctacaacga gcattgaccc tgttgaagcg attgcagaaa ttacagaaaa
     1681 gtataagttg tggctgcatg ttgattcagc ttatgccgga gttactgcaa tgattcctga
     1741 aatgaaatgg attaccaggg gatgggatcg tgcagattca ttagtcataa atccacataa
     1801 gtggatgttt actccaatgg atttaagtgt ttactttacc cgcaaacctg atattttaaa
     1861 gcgcgcattt agtttgtcag cagaatatct aaaaacaaat caagatagcg aagttgaaaa
     1921 cttaatggat tatggaattc aacttggcag aagattccgc tcgcttaaac tctggtttat
     1981 tataagatat tttggtgtta acggattatc cgaaagaatt aaaaatcaca tcaatcttgc
     2041 aaaagagctg gaaagctgga ttaaaaatga aagtgatttt gaattgatgg cacctgttcc
     2101 attttcaaca gtgtgtttta gatatcatcc agcaaacaaa gatgaaaatg aacttaatga
     2161 aatcaatgaa aatcttttag aaaaaataaa tgcatcagga aaaatatttt tatctcacac
     2221 aaaactgaat ggtaagttta caataagatt aacaattgga agtatcagac acgaacgaag
     2281 gcatattgaa gaagcctggg agcttataaa gtcaatagct cgcggatgac gctgatttaa
     2341 cgaattatta cggattaaaa attatggatt caattttaca attttctttg cttgctttta
     2401 cctctttatt cacaatggta aacccacttg gagttattcc tgtttttact actttaacaa
     2461 ccggaatgac atctaaacaa gctgcctcga ttgctcttaa agcaacatca actgctttga
     2521 ttgtactatt gctttttact ttcggcggta actttatttt tgatattttt aatatttcgg
     2581 taaacggctt aagagtagtt ggtggaatat tattctttct ttccggttac gatatgttaa
     2641 gaggtaaatt aaccagaata aaatcggatg aagagaaaga tgatgaggca gttaaaaatt
     2701 ttgcaatcgc tccacttggt gttccaatga ttacaggtcc cggtgtaatt acaatttcaa
     2761 tagtaatgat gaatgatgca ccggacgtaa ttcacagatc tttattgatt acatcaattt
     2821 ttgttgtaat gggattaacg catctcatac tattaagttc aagaaaaatt actgcatttc
     2881 ttggagaaag cggcaataaa gttcttacca gaataatggg tttaattgta atggtaattg
     2941 cagttgaatt attctttaga ggattaaaac caattataca ggatattttt ttaatcaaat
     3001 aacttttgta accaaagatg cgattgggca tcatacaata agaaaataaa ataaaataaa
     3061 tttaatttga ggaataaatg gcaacgaaaa aagctctggt aaaacaagtt aaaggaataa
     3121 cttttgtggg taaaactgat tcaaatcact ggattacaat ggatggtccc gaaagttttg
     3181 gtggaagtga tgcaggaatt cgacctaaag aattattgat gttaagtctt ggtggatgca
     3241 ctggaagtga tgttacaagt attcttggaa aaaagaagat taaactcgat ggttttgaaa
     3301 ttaatattac ggctgattct gcagatgagc atccgcaggt ttttaccagg atgcatcttg
     3361 aatatgtttt ttatggaaag aatctgccaa ttaaagatat tgaaagagca attgaattat
     3421 ctttaacgaa atattgcagt gttacagcta tgcttcaaaa ggccatgcca attgaacaca
     3481 gttacaaaat agttgaagtt gaataaataa tatttgagga attggagtaa tgtatatttg
     3541 aattacatta ctccttaaat tcttaggatt aacttttctg ataaagccct aaagtatttc
     3601 catcaggatc gttgaaaagt gcataccaac ccatggcagg aattacggtt ttcgttcttt
     3661 taacagctcc gccattttct tttaccttct ttaatattga atcgatatcc ggcatattaa
     3721 catgaaaaac agttgcctca ccttttgcaa cttcgtcaac ttttcttaat ccgaccattg
     3781 tgcctttatg attattaaaa agtaagtatc cgtttccaaa tggtttaaat tcccagccaa
     3841 gaaccttatg ataaaaatcc tttgatt
//
LOCUS       DASWBY010000641         4173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_61560, whole genome shotgun sequence.
ACCESSION   DASWBY010000641 DASWBY010000000
VERSION     DASWBY010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61560"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VF870_11670"
     CDS             <1..529
                     /locus_tag="VF870_11670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9252894.1"
                     /translation="IAWFSPELFGEERLPMLLPSLWTFIPYLLIMILLGGGQEEFGWR
                     GYALPILESKFGIWFANMILGIIHAFWHLLLWFITGTSQTYMNFGGFILLIVGYSYIL
                     SWIRKISGNRPFSGLYAHGLANAFIPLVPTLIMQKNVYQPRFWIWVSLTFIIGIIITI
                     IRERKATPLIKIFDR"
     gene            716..1576
                     /locus_tag="VF870_11675"
     CDS             716..1576
                     /locus_tag="VF870_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9252895.1"
                     /translation="MNIYKKSTRILEAVKLSSKMMISTLLILIQFSICSAQQKKSDFV
                     IGDKISFESRVLNERKTIVVIPPYYYKDRADEKFPVVYVLDPGNNLFATFGIVNYYSD
                     MLKIMPRMIIVGIVSNDRERDYLPTPSKEQPTGGGADKFLRFINSELVALIDSTYPAN
                     SERCIIGHSAGGFFAIYALENQPDLFNSFICIDPSLWYDDKSCVRKLPTFLKNNRDIK
                     KSIFISLSNEKEMGVFPFMEVLETYAPEGLKWDYIHYKSETHNSLGFKSICAGFEMIY
                     KDWKTKDDPK"
     gene            1647..2636
                     /locus_tag="VF870_11680"
     CDS             1647..2636
                     /locus_tag="VF870_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX9252896.1"
                     /translation="MKAIVHTKYGPPDELQLLEVEKPVPGDNEVLIKMHATTVTTTDC
                     NARNFTFVPQSFMFFARIMFGFKKPRIKILGIDLAGVIEAIGKDVKLFKAGDHVFGSP
                     GTKFGGHAEFVCVPENGALAIKPADLSWEKAAAISLAGNTALFFIRDLAKIQPGQTIL
                     IHAASGAIGTYAVQLAKYYGAVVTGVCSGINAALVKSLGADKVIDYTIEDFTKSDEKY
                     DFVFGVVGKTTFAQCKGLLKPDGIYLENMMEVKDFLKVLWTSIVGGKKIKGGVSAERA
                     ENLNFFVELIESGKLKPVIDKVYPLARTAEAFQYVEQGHKKGNVVISLNSERS"
     gene            2639..3568
                     /locus_tag="VF870_11685"
     CDS             2639..3568
                     /locus_tag="VF870_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9252897.1"
                     /translation="MKILVTGGTNGMGKGVAKVLAGIDNQNHEIILLGRSKELGEATI
                     RELERSTMNKKISFVLCDLARLSGVRNAIREIKSKHEYLDCIFINAGLGYAANRVETE
                     DGMDSHFQVNYLSQFMLTLNLLSLLENSENGGRVIFNVTRGGKIFWEDIQMKRNWGFE
                     NGIHQAMVAKRMFLVKLNDLYRGLKGSKVSFIGFEISKTVWSNQLNIIPVSMKIMATI
                     MKFFGTFISIEKCGMIMAPLFTESQEESLKRSGKFITWKNNEFIPMKEDESVFDREMQ
                     ERLWKISLELCNDETTARISESLQSQNFPLLQH"
     gene            3608..>4173
                     /locus_tag="VF870_11690"
     CDS             3608..>4173
                     /locus_tag="VF870_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252898.1"
                     /translation="MNKITIIIILSILLVSCANEQRKSKDEFFVFEPDIRLFTSYAFM
                     NAAGYGHDWNATMHPVRTEVREYLDSILTDDYKNEIRQYYEKFGGGDFYGYGIYAINS
                     KNPPEFGLKSDYSRNEDIARFESYDSLLQDFYERAKIAVLWERYYQKLYEINLQYKPF
                     AEIAIKQITDYCRVDSGYFRNKTNGRFY"
BASE COUNT         1395 a          667 c          882 g         1229 t
ORIGIN      
        1 tattgcctgg ttttcccctg aattatttgg ggaagagcgt ttaccaatgt tattaccttc
       61 cttatggact tttattcctt atctgttaat catgatcctt ctgggcggag gccaggaaga
      121 atttggatgg aggggttatg ctcttcctat tctcgaaagt aaattcggaa tatggttcgc
      181 aaacatgatt ttagggatca tacatgcttt ctggcatctc ctcttatggt tcataacagg
      241 tacaagtcag acttatatga atttcggagg ttttattctc ctaattgttg gttattcata
      301 tatcctttca tggattcgaa aaatctctgg caacagacca ttctcaggac tttatgccca
      361 tggacttgca aatgctttta ttcccttagt accaacatta attatgcaaa agaatgtata
      421 tcagccacgt ttttggatct gggtaagcct gacattcata atcgggatta taattaccat
      481 cattcgtgaa aggaaggcaa caccacttat caaaatattt gaccgctaat catatttccc
      541 tttgaatgaa taatgtagat tatttgtttt atattgaaat agtaaatatg gataaaacac
      601 acagactcaa aacaggattg ataacattaa caaagtcatt atttatgttc agtccggttt
      661 ttatgcatct gacagatatg gaaccatcct atataaaatt taatataata aaataatgaa
      721 catatacaaa aagtcgacaa gaattctgga ggcagttaag ttaagttcaa agatgatgat
      781 ttcaacatta ttaatcctga tacaatttag catctgttca gcccaacaaa aaaagagtga
      841 ttttgtcatt ggagataaaa taagttttga atcaagagtt cttaacgagc gaaaaactat
      901 agttgtaatt ccaccttatt attacaaaga cagagcggat gaaaaatttc ctgtagttta
      961 cgtcttagat ccggggaata atctttttgc aacattcgga attgtgaatt attattcaga
     1021 tatgttgaaa attatgcccc gaatgataat cgtgggtatt gtctccaacg atagagaaag
     1081 agattatttg cccacacctt cgaaagaaca accaactggc ggaggggctg ataaattcct
     1141 tcgttttata aattcagaat tggtagcatt aattgactca acttacccgg caaattcaga
     1201 acgttgtatt attggacatt ctgcaggagg attctttgct atttatgctt tggaaaatca
     1261 acctgactta ttcaactcat ttatatgcat tgacccaagt ttatggtacg atgataaatc
     1321 ctgtgtcagg aaattgccca cctttcttaa aaataatagg gatattaaaa aatcaatctt
     1381 catttcattg tcaaatgaaa aggaaatggg cgttttccct ttcatggaag tgcttgaaac
     1441 gtatgcaccg gaaggattaa aatgggatta tatccattat aaaagcgaaa cccataactc
     1501 attaggattt aaaagcatat gtgcgggttt tgaaatgatt tataaagatt ggaagacaaa
     1561 agatgatcca aaataacgac gaaataccaa catccgataa gtgatatgct gacaataaat
     1621 agtttaaatc cctataattt gaaataatga aagcaattgt acacacaaaa tacggaccac
     1681 ctgatgaact tcagcttctg gaggtagaaa aaccagttcc cggagacaat gaagtattga
     1741 taaaaatgca tgctacaaca gtaacaacta cggactgcaa tgcccgcaac ttcacatttg
     1801 ttccacaatc gtttatgttt tttgcaagaa taatgtttgg tttcaaaaag cccagaataa
     1861 aaattcttgg gattgatttg gcaggagtaa ttgaagccat cggaaaagat gtaaaactat
     1921 ttaaggcggg tgaccatgtt tttggatcac cagggactaa atttggtggt catgcagagt
     1981 ttgtctgtgt tcctgaaaac ggagcacttg ctataaaacc agctgatttg agttgggaga
     2041 aggccgccgc catttctttg gcaggaaata ctgcattgtt ttttataaga gatttagcaa
     2101 agatccagcc gggacagaca atacttattc atgccgcttc cggagcgata ggtacttatg
     2161 cagttcaact tgctaagtac tatggagcag tggttacagg tgtttgtagt ggcataaatg
     2221 cagctttggt gaaatcttta ggagcagata aggtaataga ttatacaata gaagatttta
     2281 ccaaaagcga tgagaaatat gattttgttt tcggtgtagt aggcaagact acttttgcgc
     2341 aatgcaaagg gttattaaaa ccggatggaa tctatcttga aaacatgatg gaagttaagg
     2401 atttcctgaa agtgctgtgg acctcaatag tcggtggtaa gaaaattaag ggaggtgtat
     2461 ctgcagaacg tgctgaaaat ctgaatttct ttgttgagct tattgaatca ggaaagttaa
     2521 aaccggttat tgacaaggtc tatccattgg caaggacagc tgaggctttt cagtatgtcg
     2581 aacaaggtca taaaaaagga aatgtagtta ttagtttgaa tagtgaaagg agttaaaaat
     2641 gaaaatatta gttactggtg gcaccaacgg aatgggaaaa ggagtggcca aagttttggc
     2701 aggaattgat aaccaaaatc atgaaatcat cctgctgggc agatcaaaag aacttggtga
     2761 ggctacaatc agagagcttg agagatccac gatgaataaa aaaatatcat ttgttttatg
     2821 tgaccttgca aggctcagcg gtgtccggaa tgccattagg gagattaaaa gtaaacacga
     2881 gtacctggat tgtatcttca taaatgcagg gttgggttat gcggcaaaca gggtggaaac
     2941 tgaagatggg atggattcac attttcaggt taactatctg tctcaattca tgctgacttt
     3001 aaacctgttg agcctgctgg agaattcaga gaacgggggg agagtgatat tcaatgtaac
     3061 ccgtggtggc aaaatattct gggaagacat ccagatgaaa agaaattggg gttttgaaaa
     3121 cgggatccat caggccatgg ttgccaaaag aatgttcctg gttaagttaa atgatttgta
     3181 cagaggattg aagggatcaa aagtatcctt cattggcttt gaaatatcta aaacagtgtg
     3241 gagcaatcag cttaatataa ttcctgtctc catgaaaatt atggcaacta taatgaagtt
     3301 tttcgggaca ttcatttcga ttgagaaatg tggtatgata atggctccgt tattcacaga
     3361 aagtcaggag gaaagcctga aaaggtcagg gaaatttatc acatggaaga ataatgagtt
     3421 tataccgatg aaggaagatg aatcagtctt tgatcgggaa atgcaggaaa gactctggaa
     3481 aatcagtctg gaattatgca atgatgaaac aactgccaga atttcggaaa gcctgcagag
     3541 tcaaaatttt cctcttctgc aacattagag aaagttaaaa acagatcaaa ttaataaaga
     3601 actgcatatg aataagataa caattattat aattctttct atactcctgg tctcctgtgc
     3661 gaatgaacaa agaaagagta aagatgagtt ttttgtattc gaacccgata taagactctt
     3721 taccagctac gcatttatga atgccgcagg ttatggccat gactggaatg ctacaatgca
     3781 tccggtcagg actgaagtca gggaatacct ggattcaata ctgaccgatg actataaaaa
     3841 tgaaattcgg cagtattatg aaaaatttgg aggtggagat ttttacggat atggtatata
     3901 tgccatcaat tcgaaaaatc cgccggaatt cgggttgaaa tctgattaca gccgtaatga
     3961 ggatatagcc aggttcgaat cttacgattc tctgctacag gatttctacg aaagagcaaa
     4021 aatagctgta ctatgggaac ggtattatca gaaactctat gagatcaacc tgcaatataa
     4081 accgtttgct gagattgcca taaaacaaat taccgattat tgccgggtgg attccggcta
     4141 tttcaggaat aaaaccaatg ggaggtttta cta
//
LOCUS       DASWBY010000642         4416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_212636, whole genome shotgun sequence.
ACCESSION   DASWBY010000642 DASWBY010000000
VERSION     DASWBY010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212636"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VF870_11695"
     CDS             complement(<1..670)
                     /locus_tag="VF870_11695"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX9252899.1"
                     /translation="MASSGYMIFAGNSNLPIAEKIAESIGLQLGALELKRFSDGEIWV
                     KFGENIRGREVFLIQSTNPPAENLLELLIMIDAARRASAKRITAIIPYFGYARQDRKD
                     QPRVSITAKLMANLITEAGADRVMTMDLHASQIQGFFDLPVDHLYGSSIFLDHLSNLS
                     DNLSVVSPDVGGIKIARAYAKMLHSGLIVIDKRRPKQNLAEVMNIIGEVEGKDILLVD
                     DLIDT"
     gene            complement(769..1431)
                     /locus_tag="VF870_11700"
     CDS             complement(769..1431)
                     /locus_tag="VF870_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252900.1"
                     /translation="MKKLILSAVVILVSLNGCLNYVQNTTIYPDGSGSMQINYWMKFS
                     DTSSVATILTLEIFNPDSIKKEFSSKFIKIKDVTTYSDTTDSTIHANIEISFNSVDSL
                     NTANAFAQSRFSLEDGASGQKIFSQFIPPVTTGFGIDGSKYHVTYNYDIRGDIITHNA
                     TNKSGNNLTWSYSLSEIGRGKTISVTFKPFKLKETPYWIYILAGLVLLIVIFFLVRKK
                     KD"
     gene            1536..1817
                     /locus_tag="VF870_11705"
     CDS             1536..1817
                     /locus_tag="VF870_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX9252901.1"
                     /translation="MPTYDYKCLKCNKTFEIFQSMKDESLKNCPDCNGELKRLIGAGA
                     GPIFKGSGFYQTDYKNSSKSSETKKSENVKPVDKSFTENKTNNKGTTEK"
     gene            complement(1843..>4416)
                     /locus_tag="VF870_11710"
     CDS             complement(1843..>4416)
                     /locus_tag="VF870_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9252902.1"
                     /translation="SGYFAYRGGQSVFYYIANKYGKEKIGELLNKIKGIGNVEEGIKA
                     TLGIDTKELGERWKKDIKKTFWPDVALREDPEDFAKRLTDPEKDKGFYNTSPTLSPQG
                     DKIAFISNRDYYWGLYVMDALTGKTIERLVEGNQVPDFEELNILTPGLTWSPDGKKLA
                     FSAKRSGVDAIYIFDVESGDREILNINFDAVESVTWSNDGKYLALIGQNARQSDIYTY
                     NFETKEIKNLTNDIFSDFDPSWSHDDKEIYFSSDRGDYLDGKLLPSNFRIAEHDYSQK
                     DIYKYILAEDKIVRITNLPFSDETSPREEVNGEELILISDLNGINNIYTKSIKSNGSD
                     DSVLNTPLKPITNSISGLYQLSLSKDTKKLVFSTLYESVFNIFMLDNPFEPKTDLDTL
                     PPTLFFTKLEESRNKKTDVQKVANETKQNKVTPPDDDEIKIFTGDVVETDSSSQNTGK
                     DFSNFVFGKESSSDEKVYANDSLFIPKDNLDNDGNYRVNKYKITFGPDLIYANAGYST
                     FYGLIGSTVLSFSDVLGDHRIVGVTGLQVDLKNSDYGLAYYYLGGRVDYGIQGFHTAR
                     FVNLVRGNFVNLYRYRSYGAYLSASLPLNRFYRVEGGISWLNVRGENLDNMAVPTDEA
                     SYFVPSIAFVHDNVMWGYTSPIQGTRYRLDAYGNPGFNDSKLSFYTLSGDYRTYLRFW
                     TDYSFVVRLSGGYSGGPNAQRFFMGGIENWINRSFATSEIPIESTSDYAFLTAVLPMR
                     GYNYSQQIGTKYFLMNYELRFPLIRYLIPGPLPILFSNILGVAYVDVGSAWNNTSNLQ
                     LFRKDLSGNTVTKDLLIGTGVGARVYFLYFLLRFDVAWAYNGEKFANKPIFYFSIGAD
                     F"
BASE COUNT         1470 a          803 c          659 g         1484 t
ORIGIN      
        1 ctgtatctat caaatcatct acaagtagaa tatcttttcc ttcaacttca ccgattatat
       61 tcattacttc agcaagattt tgttttggtc ttcttttatc aataacaatt aaaccagagt
      121 gcagcatttt agcataagct ctggctattt tgattccacc aacatcagga gaaacaactg
      181 aaagattatc tgatagatta cttaaatgat ctaagaagat tgacgatcca taaagatgat
      241 caacaggaag atcaaaaaat ccttgaatct gcgatgcgtg taaatccatc gtcatcactc
      301 tgtctgcacc ggcttcagta attagattag ccattaattt tgcggttatt gaaacacgcg
      361 gctgatcttt tctatcctgt cttgcataac caaaataagg tataatcgca gttattcttt
      421 ttgcagatgc tcttctggct gcgtctatca ttattaaaag ttctaaaaga ttttcagcag
      481 gaggattagt tgattgaatt aaaaaaactt ctcttcctct gatattctcc ccaaacttca
      541 cccatatttc accatcacta aatcttttta attctagcgc tcctaattgc aaaccaatag
      601 attctgcaat tttttctgct atcggaagat ttgaatttcc ggcaaaaatc atgtaaccgc
      661 ttgacgccat taggtgatgc tctattaaaa ttaaaaatag gtgctaaata taaccaaaga
      721 ggtaagttat gtcaataaaa ttaagtactt acacggttat aagataattt aatctttctt
      781 ttttcgtacc agaaagaaaa ttacaattaa taatactaaa ccagcaagaa tatatatcca
      841 ataaggggtt tctttaagct taaatggctt aaatgtaacc gaaatagttt taccacgacc
      901 aatttcggat aatgagtatg accaggttaa attattacca gacttattag ttgcgttgtg
      961 agtgataata tctccgcgaa tatcgtaatt atatgttaca tggtatttac ttccatctat
     1021 tccaaaacct gtggtaacag gcggaataaa ttgtgagaat attttttggc ctgaagctcc
     1081 gtcttcgagt gaaaatctgg attgtgcaaa cgcattagcg gtgtttaatg aatcaacaga
     1141 gttaaaactt atttcaatat ttgcgtggat agttgaatct gttgtgtctg aataagtagt
     1201 aacatctttt attttaataa attttgatga gaattctttt tttatagaat cgggattaaa
     1261 tatttctaat gttagtattg tggctacgct acttgtatca gaaaacttca tccaatagtt
     1321 aatttgcata cttccggagc catccggata aattgtagta ttctgaacat agtttagaca
     1381 gccatttaga gatactaaaa taacaactgc agataaaatt aactttttca aattattgat
     1441 ttcgtttatt tgagtaatga atttttaacc agccaaattt atagaacatt ctataattat
     1501 tcattatgtt tgccactaaa atttaggaga ttattttgcc aacatatgat tataaatgtt
     1561 tgaagtgtaa taagacattt gaaatctttc aatctatgaa agatgaatca ctgaagaatt
     1621 gccctgattg taatggagaa cttaaaagat taattggtgc gggcgcaggt ccaatcttta
     1681 aaggatcagg attttaccaa accgattata aaaattcatc aaaatcttca gaaacaaaaa
     1741 agtctgaaaa tgttaaacct gttgacaaat cattcactga aaataagact aataataaag
     1801 gcactactga aaaataattt ttatcagtag tactggattc tgttaaaaat ccgctccgat
     1861 tgaaaaatag aagataggct tattggcaaa tttttctccg ttataagccc aggcaacatc
     1921 aaatctcaac agaaaataaa ggaaataaac tcgagcacct acaccagttc ctattaataa
     1981 atctttagta actgtatttc cacttaaatc ttttctaaac aattgtaaat tgctggtatt
     2041 attccaagcc gaaccaacat ctacataagc aacaccaagt atattactga acaatattgg
     2101 taatggtcct ggaatcaagt aacggataag tggaaatctc agttcgtaat tcatcaagaa
     2161 atacttagta ccaatttgct gtgagtaatt gtacccgcgc attggtaaaa ctgcagttaa
     2221 aaatgcgtag tcacttgttg attctatagg aatctccgat gttgcaaatg acctgttaat
     2281 ccagttttca attccaccca taaaaaatct ttgcgcattt ggtccgccgg aatatccgcc
     2341 ggaaagtctc acaacaaagg agtagtcagt ccagaatctt aagtatgttc tgtaatcacc
     2401 actaagagta taaaagctta gtttactatc attaaaacca ggatttccat atgcatccaa
     2461 tctatatctt gttccttgaa tcggagacgt atatccccac ataacattat cgtgcacaaa
     2521 ggctatggat ggaacaaaat aagaggcctc atctgtaggt acagccatat tatcaagatt
     2581 ttctcctcta acattcaacc aacttattcc accctcaact ctgtaaaaac gattaagcgg
     2641 caaactagca gaaaggtaag caccgtaact tctatatcga taaagattaa caaaattacc
     2701 tcttaccaga ttaacaaacc gagctgtgtg aaaaccctga attccgtaat caactctgcc
     2761 acctagataa tagtaagcta aaccgtaatc gctgtttttt aaatcgactt gtaaacctgt
     2821 cacgcctact atcctatgat ctcctaaaac atcactgaat gaaagcacag tagatccaat
     2881 taatccataa aaagtactat agccggcatt cgcataaata agatcaggac cgaaggtaat
     2941 tttatattta ttaacccggt aatttccatc attatctaaa ttatcttttg gtataaacaa
     3001 tgaatcatta gcataaactt tttcatcgga tgaactttcc ttaccaaaaa caaaattaga
     3061 aaaatctttt cctgtattct gagaagatga atccgtttca acaacatcac cagtaaatat
     3121 ttttatttca tcatcatctg gaggagttac cttgttctgc tttgtttcat ttgcaacttt
     3181 ttgaacatct gtttttttat ttcttgattc ttcaagtttt gtaaaaaaca atgtgggagg
     3241 caaagtatca agatctgttt ttggttcaaa tggattatca agcataaata tattaaacac
     3301 tgactcgtaa agtgttgaaa atactaattt ctttgtgtcc tttgataatg acaattgata
     3361 gagaccactt attgaatttg taatgggttt taacggagta tttagtactg agtcatcaga
     3421 accatttgat ttaatggatt tggtgtagat attatttatt ccatttaaat cggatataag
     3481 aattaattct tcaccattta cttcttctct tggtgaggtt tcatcagaga aaggcaaatt
     3541 agtaattcta actattttat cttcagcaag tatgtattta taaatatcct tttgagaata
     3601 gtcatgctca gctattctga agttgcttgg caacaatttt ccatcaagat aatctcctct
     3661 atccgatgaa aaataaattt ctttatcatc gtgcgaccat gaaggatcaa aatcactaaa
     3721 aatatcattc gttaaatttt taatttcttt agtctcaaag ttataagtat aaatatcaga
     3781 ttgacgggca ttttgtccaa tgagtgcaag atattttccg tcattcgacc aggttacact
     3841 ttctactgca tcaaaattaa tattgagtat ttctctatct ccggactcaa catcaaaaat
     3901 ataaatggca tcaaccccgc ttcgctttgc agaaaaagct aattttttcc catcaggcga
     3961 ccaggttaaa cccggagtta aaatatttaa ttcctcaaaa tcagggacct gatttccttc
     4021 aacaagtctt tcaatcgtct ttccagttag tgcatccatt acataaagac cccagtagta
     4081 atcacgattt gatatgaatg caattttatc tccctgcgga gaaagtgttg gtgaagtatt
     4141 gtaaaaacct ttgtcctttt caggatcagt tagtcttttt gcaaaatctt caggatcttc
     4201 tcttaacgct acatcaggcc aaaaagtttt tttgatatct tttttccatc gttcgccaag
     4261 ttctttagtg tcaataccta atgtagcttt tatcccctct tcaacattac caatgccttt
     4321 tattttattt aacaactcac caattttttc ttttccatat ttattagcga tataataaaa
     4381 tactgattgt ccgcccctgt atgcaaaata tccgct
//
LOCUS       DASWBY010000643         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_362821, whole genome shotgun sequence.
ACCESSION   DASWBY010000643 DASWBY010000000
VERSION     DASWBY010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362821"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VF870_11715"
     CDS             <1..523
                     /locus_tag="VF870_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9252903.1"
                     /translation="GVGQSEVTVRSMVDFFLLVLIAGAGDELQGIKRGIIELTDAILI
                     NKAEGDNEKKANIAKSDYNNALHYLQPATKGWTSQAFTGSALTGKGIPELWDVIMKFE
                     KTIKQSGVFEQRRKDQSIQWVFRMVEDTLHDEFYNNEKVQKEIALLKQEIVKDKITPT
                     LAAEKLLSIFRTK"
     gene            complement(659..>1780)
                     /locus_tag="VF870_11720"
     CDS             complement(659..>1780)
                     /locus_tag="VF870_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9252904.1"
                     /translation="EQRSDLIIISDSDEILNKVQNEIFQTESFKSGDLAATFEKTELS
                     NFNSKLNQIKPDIIAQRITGVVFIPSTSLQSKGVEYYSSNPNNSSLFYKIKPSINKAL
                     VEIYFSGRLLTEDEIKFARKDVEINGFRVSSDENVAEEGYGNRIAMVLFSFLLYMALI
                     FSGSMTMNAVVEEKSNKIVEVLLSSATSTELMAGKILGTVIVEVIQMAIWLSPIVLLI
                     STSWFFIPQEFMPQMSFGFILYFLFNYTIALITYVALYASVGAIFDNPQDAQSGVWPL
                     LMLIMIPFFIALGMESNAQSGLAQIASLFPFSSIIVMPARMILVEVPLWQILLSCAIN
                     VIVLIGIFKLAGKIYRIGILLTGKKPKWSEVFGWLKMSS"
BASE COUNT          637 a          291 c          293 g          559 t
ORIGIN      
        1 cggtgttgga cagagtgaag ttaccgtacg ttctatggta gatttctttt tactcgtttt
       61 gattgccggc gcgggagatg aattacaggg aatcaaacgt ggcataattg aattgactga
      121 tgctattcta attaataaag ctgaaggtga taatgaaaag aaagcaaaca ttgctaaatc
      181 tgattacaat aatgcccttc actatctaca gcctgcaaca aagggatgga cctcacaggc
      241 gtttacaggc tctgcattaa caggaaaagg gattcctgaa ttgtgggatg tgattatgaa
      301 atttgagaaa actataaaac aatccggtgt atttgagcag aggagaaaag atcaatctat
      361 tcagtgggtt tttagaatgg tagaagatac tttacatgat gaattttata ataacgaaaa
      421 agtacaaaaa gaaattgcat tactaaaaca agaaattgta aaagataaaa taaccccaac
      481 acttgctgca gaaaaattgc tctcgatatt tagaacaaaa tagaattctg aattatcgga
      541 tcaactccaa atggtaatag tgattacagg gattaatcca tatctctaga ataaaagatt
      601 tcattaactc caactattaa ccaaagcgct tgtaaattgg attaaaattt aaagaatttt
      661 aagaactcat ctttaaccaa ccaaaaactt ctgaccattt aggtttttta cctgtgagca
      721 aaattccgat tcgataaatc tttcctgcta atttaaatat tcctatcaac acgattacat
      781 ttatagcaca ggaaagtaat atttgccaca aagggacttc aactaaaatc attcttgcag
      841 gcataactat tattgaagaa aatgggaaca gtgatgcaat ttgtgctaat ccactttgtg
      901 catttgattc cataccaagg gcaataaaaa atggaatcat aattaacatt agtaaaggcc
      961 agacaccaga ttgtgcatct tgcgggttat caaaaatggc tccgacggat gcataaagcg
     1021 caacataagt aataagagca attgtgtagt taaaaagaaa atatagaata aatccaaaac
     1081 tcatttgcgg cataaattcc tgaggaatga aaaaccagct ggttgatatc aacaatacaa
     1141 ttgggctcaa ccatatagcc atttgaatta cttcaactat aacagttcct aaaattttac
     1201 cagccataag ttctgtgctg gttgcagatg aaagaagcac ctctacaatt ttattacttt
     1261 tttcttctac cactgcattc attgtcattg aaccggaaaa tattaatgcc atataaagaa
     1321 ggaaagaaaa aagtaccatt gcaattctat ttccataacc ttcttcggca acgttttcat
     1381 cggatgaaac tctgaatcca tttatttcaa catcttttct tgcaaattta atttcatctt
     1441 cagttaatag tctgccggaa aaataaattt ccacaagcgc tttatttatt gatggtttga
     1501 tcttataaaa taaagatgag ttatttgggt ttgatgaata atattcaact ccttttgact
     1561 gtaacgatgt tgatggaata aatacaactc cagtaattct ttgggcaata atatccggtt
     1621 taatttgatt taacttagaa ttaaaatttg ataactctgt tttttcaaat gttgcagcta
     1681 aatcgcctga cttgaacgat tcggtttgaa atatttcatt ctgaacttta tttaatattt
     1741 catctgaatc agaaattatt atcaaatcag atctttgctc
//
LOCUS       DASWBY010000644        15547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_61739, whole genome shotgun sequence.
ACCESSION   DASWBY010000644 DASWBY010000000
VERSION     DASWBY010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 15547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15547
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61739"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VF870_11725"
     CDS             complement(<1..249)
                     /locus_tag="VF870_11725"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9252905.1"
                     /translation="MSLKEIVDFISIFYGILNYEEHSFIYSNCGHNPALYLSSSGIKL
                     LEGGDPSLNLIKSAEFKSFAIHLDKNDQILFYTDGVVEV"
     gene            complement(227..1504)
                     /locus_tag="VF870_11730"
     CDS             complement(227..1504)
                     /locus_tag="VF870_11730"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX9252906.1"
                     /translation="MKNKIELTSEIKYKLLRDISHKIRGTLDLDKILNLLLDLLSNII
                     DYDAAGIFILSEDINHPGYHQTRQKIASMVQRGFGNLPLESDAMLMEGKGIIGQTIKT
                     GKSIILNDITKDERYIPGRKETRSEITAPILRDGKTIGALNVESDNLDAFDPDDIKVL
                     NFFAEASAISIEKALLHYQILDKKKIEEQLKLAKDVQLSLLPGSEPEIPGYSFASVCI
                     QTYEIGGDYFDYIPIDENRIAIVIADVSGDGVPAALIMAAFRALLRYNAKLFSNPAKL
                     MQLMNEHVAEFMRKRDFISIFYGILNYKEHSFIYSNCGHNPAIYLSSPKIKLLEGGDP
                     SLNLINSVKFKSFEIPLNKNDQILFYTDGVVEVFSKDKKQFGLDRLQNIFNSNANKSP
                     DEIIKQIISVTKDFSSSDFYNDDFTLLVLKRNS"
     gene            complement(1595..2548)
                     /locus_tag="VF870_11735"
     CDS             complement(1595..2548)
                     /locus_tag="VF870_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9252907.1"
                     /translation="MKFFLKVLFLILITITLRAQNFPDSQNDGKYYEVNGAKLWVVNF
                     GNGEPIFFIAGGPGNAHPYLRAFDSLSTTNTLIYFDAFGRGKSDTAKDVKEYSLQRDI
                     EDLEGLRQAMGFSKINILGHSYGGLVAQGYAIEYPENVSNLILANTFHSYLMWQENCD
                     NSNHEIKTNYPEVWNDLMKVREMGAVSSDIIHQEIYSHVPYGFLYAYNPENFNRVGRK
                     PYPNSNNTKLYYQMVGRDGDFLVGSDIGTFDFRKQLKNLNMPILIYAGRYDRVAVPWM
                     EVEYKDYCPQAKFVMFEKSGHNPQVEEPEKLFKVIREFLSK"
     gene            2730..3842
                     /locus_tag="VF870_11740"
     CDS             2730..3842
                     /locus_tag="VF870_11740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9252908.1"
                     /translation="MKKIILILFLGLLLASCNVKKEQFTIIGTIEGKDSIMIFLQKRG
                     IDGWEKLDSTRVINGKFNFTGSVVLPEMWYLTMDDKKITFPLFVENSDINVQLYPDSI
                     ENSKVLGSSSQDIYKKYVVMNDTLMKKMEDVYNAWKTAKEIGDNAAMEKNDSINEDLD
                     KQVKDQLVSFIKSNNSTTVSPYLIIRNSYRFDLPELQNLISVIDTNLKASTYYQDIEK
                     RIELMRATAIGQIAPDFTLNDTTGNPVSLSSLKGKVLLVDFWAAWCGPCRAENPNVVK
                     AWKIYNKKGFEVLGVSFDTNRDKWIKAIKDDKLTWTQVSDLKGWANAAGKLYAINSIP
                     ANVLLDKDQKIIAADLRGEELLKKLEELLGAESASK"
     gene            complement(3974..6100)
                     /locus_tag="VF870_11745"
     CDS             complement(3974..6100)
                     /locus_tag="VF870_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252909.1"
                     /translation="MKLIICTILLALTWFNLCPQTLPIPPRSSNAISGSAFVSLIWSM
                     PREEREEQIYAQVIAGNIPEFMRQLSTVTANVNIGGNNHSVKYFVIPEYLAVGSDSDY
                     FLCPMTPLLAQRICNNLNCTLPTKKMVDQIYVTAPCKLRPQPIPPSDSMITVPVFAQH
                     NDSVKSIRFPVLPQYPPGTLVGGTKKDVIISNKIYQNLNINVPKPVVIYGWHQLNGIP
                     IQPVYNGHSETYADYSHGIRLVLDSVIVDANPTNFTQLLADPVLCSLVSDEGTILKPY
                     YTISGNTPPSPKTFGILWNSPSSLKIFVLPAPGSTYKAFYGNDGLAFNDSTSEFTDSI
                     LINNLSEDSMFFFRLRAQTPDGYSIPSEVLCGISSVFSSPVLVVNGFDRASSGNTYNF
                     IRQHAQAFKQNSYAFCSVTNDAVIQGLVSLQDYSIVDYILGDESTLDETFSSIEQEKV
                     KSYLKNGGNLFVSGSEVAWDLDNKGSASDKDFIHTYLKSQYVYDAPNNLAGTYYQAEP
                     VPLGIFDGTEVISYDNGTQGTINVRYPDVINGINGGTNSLQYSIVSNQFAAVNFEGIF
                     PGGVNNGKVVLVGFPFETIYPENKRTLFLSKVISFFQSPVNVSDNISLVPESFQLFQN
                     YPNPFNPSTSIQYAISSTQFVKLKVYDILGKEVATLVDDYRNAGSYEVEFNVAQESFP
                     AITSGVYFYKLQAGDFVQTKKMILLK"
     gene            complement(6115..7965)
                     /locus_tag="VF870_11750"
     CDS             complement(6115..7965)
                     /locus_tag="VF870_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252910.1"
                     /translation="MKNLLLSLLLCLSIFPQQFSRLNGLEDSQGNTILLYSLGIDNSG
                     RFSPVYKYNVDSGIETKIMDAYSNVIDTSYTNSKSIGDYEFFPNDVTNFINVGLIFDT
                     FTQGYAARNDTITFTDWGIFFVDISKQHPNQVYISDFHKVIRSFDGGYTYAADSTIEF
                     EFLSVADFDDKVFFGTDNNHLIKSEDQGITFSIVDTSDIQTSMPGLSFYYDVNQFHIY
                     RLNRSYNKFTLNVSNNKGNAFSWTKTYQSENPIFISIDSTQSGVVYLADGRKIYKSVN
                     NGYTFSEYKSLPSKLVGIYKKPNSEILYAASKYQLYEIRPDTTIIIKSVPIPAILFEY
                     YPLSIGNYWIYKVTDWSYPYYDEDTFTRKVISKEILSNGKEYFKIEEKYYNSSYINYV
                     YERADSAKGLIYQFNSDCPNPDSEKVIDDFTAEVGDSLLIQRFTMCWDSILTYFSEVG
                     SESIFNENRNFRTYEYSWLMSYTHKLAQGIGIYNIRNGYDFGESYFSLNGCVVDDIVY
                     GDTTLTDIDDPQNQIPTEFRLEQNYPNPFNPSTKISWQSPIAGWQTLKVYNVLGREVA
                     TLVDEYRNAGSYEIEFNVAQESLPAIASGVYFYRIIIGNFIETKKMILIQ"
     gene            complement(8026..8754)
                     /gene="rlmB"
                     /locus_tag="VF870_11755"
     CDS             complement(8026..8754)
                     /gene="rlmB"
                     /locus_tag="VF870_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX9252911.1"
                     /translation="MSLIIGRKPVLEALNSNEEVSQVFILFGQEGGIIDAIRVAAKKK
                     GIKVNQVPFEKFRQITQSKVAQGVAASKTEQKFYDLYEIISESKKSQYPLLLILDSIQ
                     DTHNVGAILRSADCSGVDGIIITKNNSAPINETVVKTSAGASEHVKITLIHNLAQTID
                     ELKKKGFWIVGSYLEGAKDYTSVDYKIPLAVIVGNEEKGIRKLTADKCDHLVRIPMKG
                     KIQSLNVSVATGVLLFEILRQRSI"
     gene            complement(8751..9275)
                     /locus_tag="VF870_11760"
     CDS             complement(8751..9275)
                     /locus_tag="VF870_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252912.1"
                     /translation="MALKKPKDVPQLPKAREKNIDKLKKATPAKKKSEIEFNCRIYFH
                     KDGTNGRQKYRFEIETLKIFSFLNYKITAQARKEKKNIDISLLGLTALNDYMAKVQPA
                     NVEIDFEDLFGEFTVNIIKQDGSINSVLVNFNIFKKEIELIKEFLPEKKNNSKFCTFE
                     IDKNKFTYSKEYSK"
     gene            complement(9275..10534)
                     /gene="hisS"
                     /locus_tag="VF870_11765"
     CDS             complement(9275..10534)
                     /gene="hisS"
                     /locus_tag="VF870_11765"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX9252913.1"
                     /translation="MIKAITGTKDILPVDIPKWQYLEKLLRETFQLFNYKEIRTPIFE
                     ETALFARGIGEETDIVSKEMYTFKDRSETSITLRPENTAGVVRSFIEQSLGAQQSLNK
                     LYYIGPMFRQERPQAGRLRQFHQFGAEALGSTSPLLDAEMIQVAYHILKNLELKNLVV
                     KINSLGIPEVRENYKNVLRDFLKDKKDNLSEESRKRFDTNILRIFDSKIEVDQEIMKD
                     APLLIDYLDEQSKKDFDVVKNYLTKVGIPFEVDPALVRGLDYYTKTTFEIVSGSVGSQ
                     SALCGGGRYDLLIEELGGRPTPGVGFAAGMERILLACENEKSLNLQNDSIDAYLIRID
                     SEIEIEVAKIASELRRENISCDYDYLNRSVKAQMREANKHNAKFVVFVGGDEYKRGEL
                     NVKNLSNGEQQLIKLEDFEKLKSIVKS"
     gene            complement(10662..11132)
                     /locus_tag="VF870_11770"
     CDS             complement(10662..11132)
                     /locus_tag="VF870_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX9252914.1"
                     /translation="MKVSELLKPEFIVSDLQSETKEEIINELIDLFKNDPRVEDIEKV
                     RTAVLEREKVMSTGVGKGFAIPHGKTNAVKEIVGAFGRIKDGIDYESLDGNPVNLVFL
                     LVGKDNLISTHIKLLSRISRLMNKDDFRHRLMEANSAEEIVKLFSDEEEHFLEL"
     gene            complement(11245..11730)
                     /locus_tag="VF870_11775"
     CDS             complement(11245..11730)
                     /locus_tag="VF870_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9252915.1"
                     /translation="MINKEKIELASEYFNKAYQLHMSGNIKDAIEAYKKSIGFYPTPK
                     AHTFLGWAFSLNGKFEEAIDECKIAIDLDPDFGNPYNDIGSYLINLGKHDEAIYWLEK
                     AIDAPDYEPRHFPYYNLGRVYEKQGSWHLALECYADALKVNPDYELAKTAFVRLTAMM
                     N"
     gene            11935..12612
                     /gene="folE"
                     /locus_tag="VF870_11780"
     CDS             11935..12612
                     /gene="folE"
                     /locus_tag="VF870_11780"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX9252916.1"
                     /translation="MREGPVRLAEFENEDTDHFLSSIETPIRSDAFEIDDDLKIELIE
                     KKFKEIMEVLGLDLSDDSLKDTPRRVAKMYVKETFSGLNPKNKPAVTLFDNKYKFSEM
                     LVERNVSVYSLCEHHFVPIIGKAHIAYFSSGKVVGLSKINRMVQYFSKRPQVQERLTV
                     QIANELKTMLQTEDVAVIIDADHLCVASRGVQDVNSSTVSSSYHGKFLNHNVREEFLR
                     YVYDGKK"
     gene            complement(12672..14702)
                     /locus_tag="VF870_11785"
     CDS             complement(12672..14702)
                     /locus_tag="VF870_11785"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9252917.1"
                     /translation="MDHSKHSDQRSPEKLNHTKDEHSGHSRSEHEGHTIEGFKKRFWI
                     SIIVTIPILILSPMIQHFLGIKEALRFNGDISALYVLSSFVFFYGGYPFLKGLFDELK
                     NKQPGMMTLIALAISVAYFYSSATVFGVKGEDFFWELATLIDIMLLGHWIEMKSIMGA
                     SKALEELAKLMPDEAHNIDDKGNVTDVPISEIKHEAKLLIKPGEKIPADGKIYEGNTS
                     INEAMLTGESKPVSKGEGDDVIGGSINGEGSIKIEVEKTGDETFLSQIVKMVKEAQES
                     KSKTQNLANRAAFWLTIVAITAGALTMFVWLIFTGESFNFALARTVTVMVIACPHALG
                     LAVPLVVAVSTALSAKSGLLIRNRNAFEQARNIQAIIFDKTGTLTKGEFGVTETISLD
                     NSYEEKEILKFAASVESESEHPIAQGIVRSSGDKYELKEFNSIPGKGAEGKVTGKDVK
                     VVSPGYLEENKIEIQQKDKIDKLSGEGKTVVFVLIDNKLIGGLALADIIREESKEAIK
                     QLKILGIKPMMLTGDNKQVAKWVADELGLDEYFAEVLPDKKAEKIKEVQSRGLTVAMT
                     GDGVNDAPALAQADVGIAIGAGTDVAIESADIILVKNNPKDVISLIKFSHATYKKMIQ
                     NLIWATGYNVIAIPLAAGVLYSEGIILSPALGAVLMSASTVIVAINAKLLKI"
     gene            complement(14878..15462)
                     /locus_tag="VF870_11790"
     CDS             complement(14878..15462)
                     /locus_tag="VF870_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9252918.1"
                     /translation="MKILSGAVITVLTLLVIFLLFIYSGWYNVSANEQENGIMKWVLN
                     TTKDRSIESRSKDITVPDLNDLSKLKEGFEHYNEMCVSCHGSPGLEETEVSVGLNPTA
                     PYLVKVAKEIDPKKLFWITKNGIKMTGMPAWGKTHSDEKIWAIVAFVKKLPDMTAEEY
                     QKMKSPSGTMEDDEKDPDENEPQTHSHSVEEHHH"
BASE COUNT         4952 a         2858 c         2456 g         5281 t
ORIGIN      
        1 aacttcaacc actccatctg tataaaaaag tatttgatca tttttatcca aatgaattgc
       61 aaatgattta aattcagcac tcttaataag atttaaggat ggatcaccgc cttctaataa
      121 ttttattcct gaagaactta aataaagtgc cggattatgt ccgcaattac tatagataaa
      181 agagtgttct tcataattaa gtattccata aaatattgaa atgaaatcaa ctatttcttt
      241 taaggacaag caatgtaaaa tcatcgttat aaaaatcaga tgagctgaaa tctttagtta
      301 cagaaatgat ttgtttaata atttcatcgg gagatttatt agcatttgaa ttaaaaatat
      361 tttgtaatct atctaatcca aattgttttt tatctttgct aaaaacttca accactccat
      421 ctgtataaaa aagtatttga tcatttttat tcaagggtat ttcaaatgat ttaaacttaa
      481 cactattaat aagatttaag gatggatcgc cgccttctaa taattttatt tttggtgaac
      541 ttaaataaat tgctggatta tgtccgcaat tactatagat aaacgagtgt tctttataat
      601 taagtattcc ataaaaaatt gaaatgaaat cgcgtttacg cataaactcc gcaacgtgtt
      661 cattcataag ctgcataagc ttggcgggat ttgaaaatag ttttgcgtta tatcgtagta
      721 atgctcggaa agcagccatt attaaagctg caggaacgcc gtcacctgaa acatctgcga
      781 taacaattgc tatccggttt tcatcaatag gaatgtaatc aaagtaatca ccaccgatct
      841 cataagtttg aatacaaacg ctggcaaatg aatatccggg aatttcaggt tcggatcccg
      901 gaagtaaact gagctgaaca tcttttgcga gcttaagctg ttcttcaatt tttttcttat
      961 caagtatctg ataatgcaac aatgctttct cgattgaaat tgcagaagcc tcggcaaaaa
     1021 agtttagtac tttaatatca tcaggatcga aagcatcaag attatcactc tcgacattta
     1081 gtgcccctat tgtttttcca tcacgaagta ttggagcagt tatttcagac ctggtttctt
     1141 tcctgccggg aatgtaacgc tcatctttgg ttatatcatt taggataata cttttgccgg
     1201 ttttaatcgt ctgcccgatt attcccttac cttccataag cattgcatca gattcaagag
     1261 gcagatttcc aaatccgcgt tggaccatac tcgcaatttt ttgtctggtt tgatgataac
     1321 ccggatgatt tatgtcttcg ctgagaataa aaattccagc tgcatcgtaa tcgattatat
     1381 ttgacaataa atccaataac agatttaata ttttatcaag atccagagtg ccgcggattt
     1441 tatgagaaat atcccgaagc agtttatact taatttcaga agtaagttct attttgtttt
     1501 tcatttaata gattggatta attgctgatg taaaattagc ttttaccagt ttacattcaa
     1561 acagaaaata aaagaggggc cgaagctcct taaattattt acttaaaaat tctctaatta
     1621 ctttaaataa tttttcaggt tcttctacct gcggattatg tccgcttttt tcaaacatta
     1681 cgaacttagc ctgcggacaa taatctttgt actcaacttc catccacgga actgcaacac
     1741 gatcatatct gcctgcataa atcaagattg gcatatttaa attttttaac tgctttctga
     1801 aatcaaatgt cccgatatca cttccaacta aaaaatctcc gtctcttcca accatttgat
     1861 aatacaattt tgtgttattg gaatttggat atggttttct tccaactcta ttaaaatttt
     1921 ctggattgta agcatacaaa aatccatagg gcacatggct gtaaatttct tgatgtataa
     1981 tatcacttga tacagcaccc atttctctta ctttcattaa atcattccaa acttccgggt
     2041 aattagtttt tatttcgtga tttgaattgt cacagttttc ctgccacatt aaataagaat
     2101 gaaatgtgtt tgcaagaatc aaatttgaaa cattttccgg gtattcaatt gcataacctt
     2161 gtgctacaag tccgccataa gaatgcccga gaatattaat ttttgaaaat cccattgctt
     2221 gacgcaaacc ctccaggtct tctatatctc tttgcaaaga atattctttt acatcttttg
     2281 cagtatccga ttttcctctt ccaaatgcat cgaagtaaat taaagtgtta gttgtagaaa
     2341 gagaatcgaa agcccttaga tatggatgag cattgcccgg accaccggca ataaaaaata
     2401 ttggttcgcc atttccaaaa ttaactaccc agagttttgc accatttact tcatagtatt
     2461 ttccatcatt ttgactatca ggaaagtttt gtgctcgaag tgtaatggtt atgagtatta
     2521 gaaataatac tttcaaaaag aatttcataa aacacctttt ttatttcgat gtttaaattg
     2581 agataaaaag aattgtttgt caaactttaa ttaaactaat ttaggaaggt aaactgaatc
     2641 cttcgcctca aagcatagca ttaaatagca ttctttttct aaaccaaaaa gcttattttt
     2701 atttaaataa aaactgaaag atcaagagca tgaaaaaaat aatattaatc ttatttctag
     2761 gacttttgtt agcttcctgt aatgtcaaaa aagagcagtt taccataata ggaactattg
     2821 agggcaaaga ttctattatg atttttcttc aaaaacgagg aatcgatgga tgggaaaaac
     2881 ttgactcaac tagagtaatt aatggaaaat ttaatttcac cggatcagtc gttttgcctg
     2941 agatgtggta tttaacaatg gacgataaaa aaataacctt tccacttttc gtagagaatt
     3001 ctgacattaa tgttcagctt taccctgaca gtattgaaaa ttcaaaagta cttggatcat
     3061 cttcacaaga tatctacaag aaatatgtgg ttatgaatga cacattgatg aaaaaaatgg
     3121 aggatgttta caatgcgtgg aaaactgcaa aagaaatagg cgataatgca gcgatggaaa
     3181 aaaatgattc aataaatgaa gacctcgaca agcaggtaaa ggatcagcta gtctctttta
     3241 ttaaatcgaa caatagtact acagtttcac cttatctaat aattcgcaat tcatatcggt
     3301 ttgatctgcc cgaattacag aatctaataa gcgtgatcga cactaattta aaagcttcca
     3361 catattatca ggatatcgag aaacgcattg agttaatgcg tgctactgcc attggtcaaa
     3421 ttgctccaga ttttacactg aatgacacaa ctggaaatcc agtttctctt tcatcattaa
     3481 aaggtaaagt tttattagtt gatttctggg cagcatggtg tggtccttgc cgagcagaaa
     3541 atccaaatgt tgttaaagct tggaagatat ataataaaaa aggatttgaa gtacttggtg
     3601 tttcattcga taccaaccgc gataaatgga taaaggcaat taaggatgat aaactaactt
     3661 ggactcaggt ttcagacctt aagggatggg ctaatgcagc cggtaaactc tatgcaataa
     3721 attcaattcc agccaacgtt ttgctagaca aggatcagaa aattattgct gccgacttac
     3781 gaggtgaaga attacttaaa aaacttgaag aacttctagg agcagaatca gcatcgaaat
     3841 aaaatattta tttgtggtca tttaataccg tgcaaaaact gattgatcac aatatttatc
     3901 ttctactatt aatgtaaata ataaaccctg atgcaaaggg tatcagggtt ttaattaagg
     3961 aatgttaatc aaattatttc aaaagaatca ttttctttgt ttgtacaaaa tcaccagctt
     4021 gcaacttgta aaaatatacg ccactggtga tcgcaggaaa ggattcctgt gctacattaa
     4081 actcaacttc ataactgcct gcatttctat agtcatccac taatgttgca acttcttttc
     4141 caagtatatc ataaacttta agctttacaa attgcgtact gcttattgca tactgaatac
     4201 tggtgctcgg attaaatgga ttcggatagt tttgaaaaag ctggaatgat tcggggacta
     4261 aacttatgtt atcagaaaca ttaacgggtg attgaaaaaa tgaaataact ttacttaaaa
     4321 acaaagttcg tttattttca ggataaattg tttcaaaggg aaatcctaca agaactactt
     4381 tgccgttgtt tactccacca ggaaatatgc cttcaaagtt tactgctgca aattgattcg
     4441 atacaattga atattgaaga ctatttgtgc ctccatttat tccgttaatt acatctggat
     4501 atcttacatt aattgttcct tgtgttccat tgtcgtatga gatcacttct gttccatcaa
     4561 aaataccaag tggcactggt tcggcttgat agtatgttcc tgccagatta tttggtgcat
     4621 catagacata ctgacttttt aaataagtat gaataaagtc tttatcactt gctgacccct
     4681 tgttatccaa atcccacgct acctctgaac ccgacacaaa aaggtttcct ccgtttttca
     4741 aataactctt tactttttcc tgttcaatac tgctgaacgt ttcatccagt gtgctttcat
     4801 caccaagaat ataatccaca atagaataat cttgtagtga aaccaaccct tgaattactg
     4861 catcatttgt tacagaacaa aaagcatagc tattttgttt aaatgcttgc gcatgctgac
     4921 gtataaagtt gtatgtgtta cctgaagatg ctcggtcgaa accatttact accaaaaccg
     4981 gggaactgaa tactgaacta attccacata aaacttctga aggaatagaa taaccgtccg
     5041 gggtttgggc tcttagccga aaaaaaaaca tagaatcttc tgataaatta tttattaata
     5101 tactatcagt aaactctgaa gtagaatcat taaaagctaa tccatcattt ccatagaatg
     5161 ctttgtaggt tgaaccggga gcaggaagta caaaaatctt caggctggat ggtgagttcc
     5221 aaagaatgcc aaatgtttta ggtgaaggtg gtgtattacc ggaaattgta taataaggtt
     5281 ttaaaattgt tccttcatcg ctaactaggc tgcataaaac aggatcagct aaaagctgag
     5341 tgaagttagt tggattagca tcgacaataa cagaatctaa aactaaacgg ataccgtgac
     5401 tataatctgc ataagtttca ctatgcccat tataaacagg ttgaattgga attccattca
     5461 attgatgcca accataaatt accacaggtt ttggcacatt gatatttagg ttttgataaa
     5521 ttttgtttga aattattaca tctttttttg ttccgcctac caatgtgccg ggtggatatt
     5581 gagggagaac aggaaatctt attgatttaa cagagtcatt atgctgagca aaaacaggta
     5641 cggtaatcat agaatcagat ggcggaattg gctgaggtct taatttgcaa ggggcagtaa
     5701 cataaatctg atcaaccatt ttctttgttg gaagcgtaca atttagatta ttgcaaattc
     5761 tttgagcaag caaaggagtc attgggcata aaaaataatc tgaatcggat ccgacagcga
     5821 gatactccgg aattacaaaa tattttaccg aatgattatt gcccccgata tttacattag
     5881 cagtaactgt acttaattgt ctcataaatt caggaatgtt gccagctatc acttgcgcat
     5941 aaatctgttc ttctctttcc tcccttggca tactccatat taaacttaca aatgcagaac
     6001 cggaaattgc atttgatgat ctgggcggaa ttggcaaagt ttgaggacat aaattgaacc
     6061 aggttagggc aagaagaatt gtgcaaataa ttaatttcat ttagtctatc agaattattg
     6121 gatcaaaatc attttttttg tttcgataaa attaccaatt ataattctat aaaaataaac
     6181 tccactggca atcgcaggaa gggattcctg cgctacatta aactcaattt cataactacc
     6241 tgcatttcta tattcatcaa ctagtgtagc aacttccctc cccaatacat tataaacttt
     6301 taaagtttgc caacctgcta taggcgactg ccaacttatt tttgtacttg gattgaatgg
     6361 gtttgggtag ttttgttcta atctaaattc tgtgggaatc tgattttgtg gatcgtcaat
     6421 atcagttaat gtagtatcgc cataaacaat atcatcaacc acacaaccat ttaaggaaaa
     6481 atatgactca ccaaaatcat agccatttct aatattatag ataccgatcc cctgagctaa
     6541 tttatgagta taactcatca gccaagaata ttcataagtt ctaaaatttc tattttcatt
     6601 gaaaatgctc tcgctaccca cttcagagaa ataagtaaga attgaatccc aacacattgt
     6661 aaatctttga atcaataaac tatcgccaac ttctgcagta aaatcatcaa tcactttttc
     6721 actatcagga ttaggacagt cactgttaaa ctgataaata agccccttag ctgaatcagc
     6781 tctttcatat acatagttaa tgtatgaaga gttataatat ttttcttcaa tcttaaaata
     6841 ttctttgcca ttacttagaa tttcttttga aataactttt ctggtaaaag tatcttcatc
     6901 ataatacggg taggaccaat cagtaacttt ataaatccaa taatttccaa ttgaaagagg
     6961 atagtattca aatagtattg cgggtattgg aactgatttt attataattg tagtgtctgg
     7021 tctaatttca tatagttgat atttacttgc agcataaaga atttctgagt ttggtttttt
     7081 ataaatgcca actaatttac ttggcaagga tttatattct gaaaaagtgt atccattatt
     7141 aactgattta taaatttttc tcccatcggc taagtacaca actcctgatt gtgtggaatc
     7201 gattgaaatg aaaattggat tttcactctg ataagttttt gtccaggaaa aagcattacc
     7261 tttgttgttt gagacattta gagtgaattt attgtacgat ctgtttaatc tataaatatg
     7321 aaattgattt acgtcataat agaatgagag tcctggcata ctagtttgaa tatcagaagt
     7381 atcaactatt gagaaagtaa ttccctgatc ttcagatttg atcaaatgat tattatctgt
     7441 tccgaaaaat actttatcat caaaatctgc aaccgataaa aattcaaact caattgtaga
     7501 atcggctgca taagtatatc cgccatcaaa acttctaatt actttgtgaa aatcagaaat
     7561 gtaaacttga tttgggtgct gtttagaaat atcaacaaag aatattcccc aatcagtaaa
     7621 tgtaattgta tcatttcttg ctgcatatcc ctgagtaaat gtatcgaata taaggccgac
     7681 attaataaaa ttagttacat cattagggaa aaattcatag tcaccaattg attttgaatt
     7741 tgtgtaagat gtatctatta cattggaata cgcatccata atttttgttt cgattcctga
     7801 atcaacatta tacttataga ctgggctgaa acgtccagaa ttatcaatgc ctaatgagta
     7861 aagcagaatt gtatttccct gagaatcttc aagaccattc aatcttgaaa attgttgtgg
     7921 aaaaatcgaa aggcaaagca atagagatag taagagattt ttcatatgaa ctccctaaaa
     7981 aatctattca aacgtatgca ataaattctt gaaaggcaat taatactata tacttctctg
     8041 tcgtaatatt tcaaaaagca gtacccctgt tgcaacagaa acatttaatg actggatttt
     8101 ccctttcatc ggtatgcgga caagatgatc gcatttatca gcggtaagtt ttcttattcc
     8161 cttttcttca ttaccgacaa ttactgcaag tggaattttg taatcaacag aagtgtaatc
     8221 tttcgcgcct tcaagataag aaccaacaat ccagaatccc tttttcttta gttcatcgat
     8281 tgtctgagca agattgtgta taagtgtgat cttaacatgc tcgcttgcac ctgcagatgt
     8341 tttaactacg gtttcattta tcggagccga attatttttt gtaattataa ttccgtcaac
     8401 accgctgcag tccgcactgc gcagtattgc tccaacattg tgcgtatcct gaattgaatc
     8461 taaaattaaa agaagaggat attgggattt ttttgattcg ctgataattt cgtaaagatc
     8521 ataaaatttc tgctcagttt ttgaagctgc aacaccttgt gcaactttac tttgtgttat
     8581 ctgtctaaat ttttcaaacg gaacctggtt tactttaata ccctttttct ttgcagccac
     8641 gcggattgca tctataattc cgccttcctg cccgaacaaa ataaaaacct gcgaaacttc
     8701 ttcattagaa ttgagtgctt ctaaaacagg tttccttccg ataattaaac tcacttactg
     8761 tattcctttg agtatgtaaa tttattttta tcaatttcga aagtacagaa tttgctgttg
     8821 ttctttttct ctggtaagaa ttcttttata agttcaattt cttttttgaa gatgttaaaa
     8881 tttacaagta cgctgttaat gcttccatcc tgtttgataa tatttacagt aaactcaccg
     8941 aataaatctt caaaatcaat ttcaacattt gccggttgta ccttagccat ataatcattc
     9001 agcgcagtta aaccgagtaa actgatatca atgttttttt tctctttgcg agcctgtgcg
     9061 gtaattttat aattaaggaa ggaaaatatt tttagagtct ctatttcaaa tctgtatttc
     9121 tgcctgccgt tcgttccgtc tttatgaaaa tagattctgc agttaaactc aatttcggat
     9181 ttcttttttg caggagtagc ttttttgagt ttatctatat tcttttcccg tgcttttggt
     9241 aattgcggaa catcttttgg tttttttaat gccattaact tttaactatt gatttaagtt
     9301 tttcgaaatc ttcaagctta attaattgct gttcaccgtt agaaagattt ttcacattca
     9361 actcaccacg cttgtattca tcaccgccaa caaacactac aaactttgca ttgtgtttat
     9421 tggcttcacg catttgagct ttaacactcc tgttcagata atcataatca cagctaatat
     9481 tttctctgcg cagttctgaa gcaatttttg caacctcaat ctcaatttcc gaatctattc
     9541 tgattaaata tgcatcaata gaatcatttt gcaggttcaa tgatttttca ttttcgcaag
     9601 cgagaagaat tctttccatc cctgctgcaa agccaacacc gggagttggc ctaccgccaa
     9661 gttcttcaat aagtaaatca tatcttccgc cgccgcacaa tgcactttga gatccaacac
     9721 ttccgcttac aatttcaaaa gtagttttcg tgtagtaatc taatccacgt accaacgcgg
     9781 gatcaacttc aaacggaatt ccgacttttg tcaaataatt tttaacaaca tcaaaatcct
     9841 ttttactttg ttcatcaaga taatctatca gtaaaggtgc atctttcatt atttcttgat
     9901 caacttcaat cttgctatca aatattctaa ggatgtttgt atcaaatctt tttctgcttt
     9961 cttcggatag attatctttt ttgtctttaa gaaaatcacg aagaacattt ttataatttt
    10021 ctctcacttc aggaattccg agcgagttta tcttaacaac caaatttttt aattcaagat
    10081 ttttcaggat atgataagca acctgaatca tttctgcatc gagtaacgga gaagtacttc
    10141 ctaatgcctc tgcaccaaat tgatggaact gtctcagcct tccagcctgg ggtctttcct
    10201 gcctgaacat cggtccgata tagtacaact tatttagtga ctgctgtgca ccgagtgact
    10261 gctcaataaa agatcgaaca acccctgcag tattttccgg acgaagcgta atgctggttt
    10321 cacttctgtc tttaaaggtg tacatctctt tgcttacgat atcagtttct tcaccgatac
    10381 cgcgtgcaaa taaagctgtt tcctcaaaaa ttggagtacg aatttcttta tagttaaata
    10441 attgaaatgt ttccctcaga agtttttcca ggtattgcca tttaggaata tctactggca
    10501 gaatgtcttt tgttccggtt atcgctttga tcattatttt gtctgaatca tttctctcac
    10561 atactcgtca tcccaagcgg agtcgaggga cgaagtgtga ggcattatag aataataagg
    10621 catggtcatc tttcgactta gctcaaagat gacatcagat attacagctc taaaaagtgt
    10681 tcctcttcat cactaaaaag ctttacaatt tcttcagcac tgtttgcttc cataagacga
    10741 tgtctgaaat catctttatt cataagccgt gaaattctgc ttaacaactt aatgtgcgtg
    10801 cttatcaaat tatctttccc aaccagaagg aaaacaagat tcacaggatt tccatcaaga
    10861 gactcataat caattccatc tttaattctg ccaaatgctc cgacaatttc tttaactgca
    10921 tttgtttttc cgtgaggaat tgcaaatcct tttcccactc ccgtagacat cactttttca
    10981 cgttcaagaa cggcagttct tactttttca atatcttcaa cacgaggatc atttttaaag
    11041 agatcaatga gttcattaat tatttcttct tttgtttcac tctgtagatc agaaacaata
    11101 aactctggtt ttaatagttc gcttactttc attttttctc agattggatt taatcagatt
    11161 ataaaaaaaa tcatttatct ggcaggtaat ttaacaatca caagatagtt ttacaattag
    11221 attatcagat ataatccgaa caaactaatt catcatagct gttaatctta caaaagcggt
    11281 ttttgcaagt tcataatcag gatttacttt tagcgcatct gcataacact ctaaagctaa
    11341 atgccaggaa ccttgtttct cataaactct tccaagattg taatatggaa aatgacgcgg
    11401 ttcataatca ggagcatcga ttgccttttc gagccaatag attgcctcat catgctttcc
    11461 aagatttata agataagatc caatatcatt ataaggattt ccaaaatcag gatcgagatc
    11521 aatggcaatt ttacactcat caattgcttc ttcaaacttg ccgttcaaac taaatgccca
    11581 gcctaaaaaa gtatgtgctt ttggtgtggg ataaaatccg attgattttt tatatgcctc
    11641 aatcgcatct ttaatattgc cgctcatatg aagctgatag gctttattga aatactcaga
    11701 agctaattct attttttctt tgttgatcat tacgattcat ataatccatt tgaacttatg
    11761 cataaataaa tatttcattt gttaatttca attatttaag agagttcgtg ataataatca
    11821 taacatttgc cttattatcg aaataattat aaagacatag aaaattactc tacaaaatgg
    11881 aactatataa atttagaatt ggttttgatt ctaacattat aaaggaaatt cgatatgaga
    11941 gaaggtccgg taagattagc agagtttgaa aatgaagata ctgatcattt tctaagttcg
    12001 atagaaactc ctatccgaag tgatgcattt gaaattgatg atgacttaaa aattgaactg
    12061 attgaaaaaa agttcaaaga aattatggaa gtgcttggat tagatttaag cgatgatagt
    12121 ttgaaagata ctccacgcag agttgcgaaa atgtatgtta aagaaacatt tagcggatta
    12181 aatccaaaaa acaaaccggc ggttacttta tttgataaca aatataagtt cagcgaaatg
    12241 cttgtggaaa gaaatgtttc agtgtactcg ttatgcgaac atcattttgt tccgatcatc
    12301 ggcaaagcac atattgcata tttctcaagc gggaaagttg taggtctttc aaagattaac
    12361 agaatggttc agtacttttc taaacgaccg caggtacagg aaagattaac ggttcaaatt
    12421 gctaatgagc ttaaaacaat gctgcaaacc gaagacgtag cagtaattat tgatgctgat
    12481 cacctgtgcg tagcttcacg cggagtacaa gatgtaaata gctcaacagt ttcatcaagt
    12541 tatcacggta aatttttaaa ccacaatgtc agagaagaat ttttacgata tgtttacgat
    12601 ggtaagaaat agatttcgat gaataactga tcccaaaaaa gccccgatag agtcggggct
    12661 ttttgtttta attaaatctt caacaactta gcatttatcg caacaattac cgtacttgca
    12721 gacataagca ccgcaccaag tgctggactt aaaattatgc cttcagaata caaaacacct
    12781 gcagcaagag gaatagctat aacattgtag ccagttgccc aaatcaaatt ctggatcatc
    12841 tttttatatg tagcatgtga aaatttaatt aatgaaatta catctttggg attattcttt
    12901 acaagaatta tatctgcaga ttcaatagca acatcagttc cagcacctat agcaatacca
    12961 acatcagctt gtgcaagtgc gggtgcatcg ttcacaccat caccggtcat tgcaacagtc
    13021 aatccacggc tttgcacttc ttttatcttc tcggcttttt tatccggcag tacctcagca
    13081 aaatattcat cgagcccgag ttcatcagca acccatttag caacttgttt gttatctcca
    13141 gtaagcatca ttggttttat tcctaatatc ttaagctgct taattgcttc cttcgattct
    13201 tccctgatta tatctgctag cgctagtccg ccgattagtt tattatcaat aagaacaaat
    13261 acaacggtct ttccttcgcc agataattta tcaatcttat ctttttgttg aatctcaatt
    13321 ttattttctt caagatatcc cggactcaca accttaacat ctttgccggt tacttttcct
    13381 tctgctcctt tgccgggaat cgaattaaat tcttttaatt catatttatc tccagaagat
    13441 cttacgattc cctgtgcaat cggatgttct gattcagatt caactgaagc agcaaatttc
    13501 aaaatttctt tttcttcata actgttatca agagaaattg tttccgttac tccgaattct
    13561 ccttttgtta atgttcctgt cttatcaaaa ataattgcct gaatatttct tgcctgctcg
    13621 aatgcatttc tattccttat tagcaaacca cttttagcag aaagtgcagt tgagacagct
    13681 accacaagag gaaccgctag accaagagca tgcggacaag caattaccat tacagttaca
    13741 gttctggcta atgcaaagtt gaaactttct ccggtaaaaa taagccatac aaacattgtt
    13801 aatgcacctg cggtaattgc aactatagtt aaccagaatg ctgctcggtt agcaaggttc
    13861 tgagttttgg atttactctc ctgagcttcc ttaaccatct ttacaatttg ggaaagaaaa
    13921 gtttcatctc cggttttttc gacttcaatt ttgattgagc cttcgccatt tattgatcct
    13981 ccaattacat catcaccttc accctttgaa acaggttttg attcacctgt taacattgcc
    14041 tcattaattg atgtatttcc ttcataaatt tttccatcag caggaatttt ttcaccgggt
    14101 tttattaata atttagcttc gtgtttaatt tcggaaattg gaacatcagt aacatttcct
    14161 ttatcgtcaa tattatgggc ttcatccggc ataagtttgg caagttcttc aagagctttt
    14221 gaagcaccca ttattgattt catttcaatc cagtggccaa gaagcataat atcaataaga
    14281 gttgccagct cccagaaaaa atcttcacct ttaacaccaa acacagttgc actactgtag
    14341 aaataagcaa ccgaaattgc aagtgcaatc aaagtcatca ttcccggctg cttgtttttc
    14401 aattcatcaa acagaccttt taagaaagga tagccgccat agaaaaagac gaaagacgaa
    14461 agcacataca aagcagagat atctccatta aaacgcaaag cttcttttat ccccaaaaaa
    14521 tgctgaatca ttggtgagag aataagtatc gggatcgtaa ctataattga aatccagaac
    14581 cgcttcttga agccttcaat tgtatgtccc tcatgctcag aacggctatg tccgctgtgc
    14641 tcatcctttg tgtggttcag cttctcaggc gatctttgat cactatgttt agaatggtcc
    14701 atttttgaat gattttcatg cttcatattg cgagtattat gattattatt ttgatgttca
    14761 ttttccatat taaaatcttg cattaataat taaatttttt cttaactaaa ataattttta
    14821 ctatacttct aagtattaca tttaattgga aatcttttac agaattctca tagtgattca
    14881 atgatggtgc tcttccacag aatgtgaatg cgtttgcggc tcattttcat ccggatcttt
    14941 ttcatcatct tccatagttc ctgatggact tttcattttt tgatattctt cagcagtcat
    15001 atccggaagc tttttcacaa atgctacaat agcccagatt ttttcatctg aatgggtttt
    15061 accccaggca ggcattcctg tcatttttat tccgtttttt gtaatccaga ataatttctt
    15121 gggatcaatt tcttttgcta ctttaactaa atatggtgct gttggattta atcctaccga
    15181 cacttcagtt tcttctaacc ctggcgaacc atgacagcta acgcacattt cattgtaatg
    15241 ctcaaaacct tcctttaact ttgataaatc atttaagtca ggaacagtaa tatcttttga
    15301 gcgggattca attgatctat cctttgtggt atttaaaacc catttcatta tcccattttc
    15361 ctgttcattt gcacttacat tataccaacc actgtaaata aataatagaa aaattactaa
    15421 cagagttaat accgttataa cagcacccga taagattttc atagggccct cctttgatta
    15481 aaagatgaca tctctttaaa ggatgtcatc ttaaacaact tatatattca taatttataa
    15541 atattta
//
LOCUS       DASWBY010000645         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_61798, whole genome shotgun sequence.
ACCESSION   DASWBY010000645 DASWBY010000000
VERSION     DASWBY010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61798"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VF870_11795"
     CDS             complement(<1..1236)
                     /locus_tag="VF870_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252919.1"
                     /translation="MKRISKIVLLDASMFLLYSCGGILDTSVKEVFLNDNWLVNSEND
                     SIKLTTNIPNEINFNLYINKVIPHPFFGDNEKNIQWVSENDWKYEKEFDVDSSFLTNE
                     NIELIFEGIDTYSDIYLNDIKILSTDNQFRQWKADVKSRLQPSNNKLRILIRSPFDIE
                     KQKAEAYGFQPPSDSRMFTRKAAYVYGWDWGPRIVTSGLWKSVKLESWNGPRLKDVFI
                     IKDSLTERKAYLTARFEISSNKETEISLFVYGHRKKIMTTEGDQTVEINFEIDSPNLW
                     WPNGLGEQKLYAIDCYLAHNNYGVQTISKKIGLRTIELVQEKDSIGKSFYFKVNGQPV
                     FMKGANYIPMQMIGNDVDTNKYARLINDTAESNMNMLRVWGGGIYEHDIFYDLCDEKG
                     ILVWQDFMFANSMYPGDSLM"
     gene            complement(1263..>1991)
                     /locus_tag="VF870_11800"
     CDS             complement(1263..>1991)
                     /locus_tag="VF870_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9252920.1"
                     /translation="IIFIHGFPYDSTMWKAQVDELSDKYFCVAYDIRGLGDSPAGDGQ
                     YTMESFVDDLESIINELGLDKPILCGLSMGGYISLRALERMQNKFSAVVLCDTRSEAD
                     NSEGKLKRAAAIKRINTEGLAPFAKEFITNCYGDHYKQKDKNDFENQIAKSSAFDPIG
                     VKGSLLAMLGRNDTTEYLSKIKIPALVICGEHDALTPPLVMKSMAEKINGAEFVIIKN
                     SGHMSPIENPLEVNKTIIDFLKKL"
BASE COUNT          623 a          382 c          291 g          695 t
ORIGIN      
        1 cattaatgaa tcaccgggat acatactatt tgcaaacata aaatcctgcc agacaagaat
       61 tcctttttca tcacatagat cataaaaaat atcgtgctca taaattccac cgccccatac
      121 tctaagcata ttcatattcg attcagcagt atcatttatc aatcttgcgt acttatttgt
      181 atcaacatca tttccaatca tttgcattgg aatgtaattg gcacccttca taaatacagg
      241 ttgcccgtta actttaaagt aaaacgattt tccaattgaa tctttttcct gtacaagttc
      301 aattgttctt aagccaattt tttttgagat tgtttgaaca ccataattat tatgagctaa
      361 ataacaatca atagcatata atttttgctc acccagacca tttggccacc ataagttcgg
      421 agaatcaatt tcaaaattga tttcaactgt ttgatcacct tctgtggtca tgattttctt
      481 tcgatgaccg taaacaaata atgatatttc tgtctcttta tttgatgaga tttcaaatct
      541 ggcggttaga tatgcttttc tttcggtgag agaatcctta attataaata catcttttaa
      601 ccgcggtcca ttccaacttt ctaatttaac ggatttccag aggcctgaag taacaatgcg
      661 cggaccccaa tcccagccat aaacataagc tgcttttctg gtaaacattc tcgaatcact
      721 gggcggctga aagccatatg cttcagcttt ttgtttttca atatcaaaag gagatcggat
      781 gagaatacgt aatttattat tggatggttg aagtctagat tttacatcag ctttccattg
      841 ccggaactga ttatcggttg aaaggatttt aatgtcattt aaataaatat ccgagtaagt
      901 atcaattcct tcaaaaatca gctcaatatt ttcatttgtt aaaaaagaag aatcaacatc
      961 aaattctttt tcatatttcc agtcattttc agaaacccat tgaatgtttt tttcattgtc
     1021 tccaaagaac ggatgaggaa ttactttgtt tatatataaa ttgaagttta tttcattggg
     1081 aatattagtg gttaatttga ttgaatcatt ttctgaatta accaaccaat tgtcattcaa
     1141 aaaaacttct tttactgatg tatccaaaat tcctccgcag gaataaagca aaaacattga
     1201 tgcatcgagt aaaactattt ttgaaattct tttcatcttt agttataaca caaacaaaaa
     1261 aattataact tttttaaaaa atcgattatt gttttattta cttccaaagg attttcgatg
     1321 gggctcatat gtccgctgtt tttaattatg acaaactcag caccatttat cttttcagcc
     1381 attgatttca ttaccaacgg tggcgttaag gcgtcgtgct cgccgcaaat taccaaagca
     1441 ggaattttaa ttttacttag atattcagtc gtatcgtttc ttcccagcat tgcaagcaga
     1501 gaacctttaa caccaatggg atcaaatgct gaagactttg caatttgatt ctcaaaatca
     1561 tttttatctt tttgtttata atggtctcca tagcagtttg ttataaattc ttttgcaaag
     1621 ggggcaaggc cttcggtgtt tatccttttt atagctgcag ctcgttttaa ctttccttca
     1681 ctattatcgg cttcagaacg agtatcacac aaaacaacag cagaaaattt attctgcatt
     1741 ctttcaagtg cgcgtagtga aatatagccg cccatcgata gtccgcacaa aattggtttg
     1801 tctaagccca gctcatttat tattgattca agatcgtcta caaaggattc cattgtatat
     1861 tgtccatcac cagcaggaga atctccaaga cctcgaatat catacgcaac acaaaaatat
     1921 ttatcactta attcatcaac ctgcgctttc cacattgtgc tatcgtaagg aaaaccatgt
     1981 ataaatatta t
//
LOCUS       DASWBY010000646         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_212967, whole genome shotgun sequence.
ACCESSION   DASWBY010000646 DASWBY010000000
VERSION     DASWBY010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212967"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VF870_11805"
     CDS             complement(<1..590)
                     /locus_tag="VF870_11805"
                     /inference="COORDINATES: protein motif:HMM:NF040322.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252921.1"
                     /translation="MKNIYFLFIVITITNFACAQQNNDTLFVAHWNVENLFDTIDDPK
                     TVDEEFLPTSEKEWNEDRLDKKLYNLSRVIRSMNYDKGPDLLGVCEVEHQALLDTMIA
                     KYLSDKSYKTAYMESPDGRGIDNGIIYNSKKLSLLKITGHRVDTGGGYETRLVLEGTF
                     LFEKTDTIYFFVNHWPSRRGGEDTSEPRRISAAKTLR"
     gene            complement(618..2438)
                     /locus_tag="VF870_11810"
     CDS             complement(618..2438)
                     /locus_tag="VF870_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX9252922.1"
                     /translation="MKSAHSNPAKDIIKAMGIVFGDIGTSPIYTLSIIFTLTIPSREN
                     IYGVLSLIFWTLITLVTIQYAWLAMSMSTRGEGGIIVLKEILVSTLKKGRKVGFISFL
                     GYIGVSLLLGDGVITPAISILSAVEGLKLIPGLESTGLRTIIIITSVITILLFAIQFK
                     GTSKVALSFGPIMLLWFISLFLSGFFYLIHMPDILLAFNPLYAIKFFIHNGLPGFFVL
                     SEVILCATGGEALYADMGHLGGKPIRQAWSFVFFALIINYFGQGAYILEHNDTNQVLF
                     KLVSTFSDVFYIPFLILTLFATIIASQAMISAVMSLIFQGINIGIFPLMKIKYTSTEM
                     RSQIYLPSVNWLLLLAVLLMILVFKESANLAAAYGLAVTATMFISSIFIVSILRNRKE
                     YIKMSIAICVLIITAVYLVAVTHKIPYGGYWSVIIATIVLLIMGLWITGMNRLRKYLR
                     SVNLDVFVESFKQIYETGNYVKGEALFFTKSVNVVSPYILHCMFRTGIMYEKNVLVSV
                     ENTDVPYGVQLERYEEVSPGLFSARITYGYMEVPDLPKLFKEWKFNEKAIFYGAEEIK
                     TNRFFLKFFVVLKKIAPNWISYFDFPYNKLHGVVTRIEIQ"
     gene            2642..>3340
                     /locus_tag="VF870_11815"
     CDS             2642..>3340
                     /locus_tag="VF870_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX9252923.1"
                     /translation="MKNKKFVIIDAMALAYRAYFAFISRPLTNSKGESTSAVYGFLNQ
                     LIKVFEDHKPDYLAVAFDSKEKTFRHEKYEAYKSSRDAMPDDMIPQLQRIKDIVIALN
                     IPLYILPRYEADDIVGTAVKKAEELGMLSYAITPDKDYFQLITDKTNVVRPGKKSDEA
                     ILYDKQKVIDELGFEPKYMVDYLALVGDSSDDIPGVAGIGPKGAVPLIQKYGTIENIY
                     RHIDEIEKAGIKKKL"
BASE COUNT         1152 a          589 c          518 g         1081 t
ORIGIN      
        1 cgtaatgttt ttgcagcact gattcttctt ggttctgatg tatcctcacc ccctctcctg
       61 gaaggccagt gatttacaaa aaagtatata gtgtctgtct tttcaaataa gaaagttcct
      121 tctagaacta agcgtgtttc atacccgcca cctgtatcaa ctctatgccc tgtaattttt
      181 aataatgaaa gtttttttga gttataaatt attccattgt caattcctct tccgtcgggt
      241 gattccatat atgctgtttt gtatgattta tcagatagat attttgctat cattgtatct
      301 aataatgcct gatgctcaac ttcgcagaca cccagtaaat caggtccttt atcataattc
      361 atagatcgaa tgactctgga cagattatat aattttttgt caagacgatc ttcattccat
      421 tctttttcac tcgttggcag aaattcttcg tctacagttt tgggatcatc gattgtgtca
      481 aaaagatttt caacattcca gtgtgcaaca aatagtgtat cattattttg ttgggcacaa
      541 gcaaaatttg ttattgtaat tacgataaat aaaaaataaa tatttttcat atgataattt
      601 tctcaaagat aaaaagatta ttggatttca attcttgtaa cgacaccgtg aagtttattg
      661 tatgggaaat caaaatagct gatccagtta ggcgcaattt tctttagtac aacaaaaaac
      721 tttaagaaaa atctgttagt tttaatttct tctgcgccat aaaaaatagc tttctcatta
      781 aacttccatt ctttaaatag ttttggaaga tcaggtactt ccatataccc ataagtaata
      841 cgagcagaaa ataaacccgg acttacttct tcatatcttt caagttgaac accataaggc
      901 acatctgtat tttcaactga aactaagaca tttttctcat acattatccc ggttctaaac
      961 atacaatgca aaatataggg tgaaacaaca ttgacacttt ttgtaaaaaa taaagcttca
     1021 ccttttacat aattacctgt ttcgtatatc tgcttaaagc tttcaacaaa tacgtctaaa
     1081 ttaaccgaac gcagatattt tctcagtcta ttcattccgg ttatccacaa tcccattatt
     1141 aacagaacaa ttgtggcaat aataactgac caatatccgc catagggaat tttatgtgtt
     1201 acggcaacaa gataaactgc tgtgataatc aaaacacaaa tggcgataga cattttaatg
     1261 tattcttttc tattccttaa aatagaaact ataaatatgg aacttataaa cattgttgca
     1321 gttactgcta agccgtatgc tgccgccaga ttagctgatt ctttaaatac taaaatcatt
     1381 aacagcacag ctaataaaag aagccaattt acagatggaa gataaatctg tgatctcatt
     1441 tccgtagatg tgtattttat tttcatcagt ggaaatattc caatattaat tccttgaaag
     1501 attaaagaca ttacagcact gatcatcgcc tgcgaagcaa ttattgttgc aaataatgtt
     1561 aagattaaaa atggtatata gaatacatct gaaaatgtgc tcactaattt gaacagaacc
     1621 tgatttgtat cgttatgctc aagaatatat gcaccttgtc caaaataatt gattatcaat
     1681 gcgaagaaaa caaaagacca cgcttgtcgt atcggtttac caccaagatg tcccatatct
     1741 gcatataaag cttcaccgcc ggtagcacaa aggattactt cacttaaaac aaaaaatcct
     1801 ggcaaaccat tatgaataaa aaatttaatt gcatacaaag gattgaatgc taataatata
     1861 tcaggcatat gtatcaaata aaaaaatcct gaaaggaaaa gacttatgaa ccacaataac
     1921 ataatcgggc cgaaggacaa ggcaactttt gaagtgcctt taaactgaat ggcaaataat
     1981 aaaatcgtaa ttactgatgt tatgattata atcgttctta atccggttga ttccaatccg
     2041 ggaataagct ttaatccttc aactgcagaa agaatactta tagcaggagt tataactcca
     2101 tcaccaagaa gtaaagaaac acctatgtaa ccaagaaatg aaatgaaacc aactttcctt
     2161 cccttcttta aagtcgatac aagaatttct ttcagaacta taattccgcc ttcacctctt
     2221 gtactcatac tcattgcaag ccaggcatat tgaattgtga caagagtgat aagtgtccag
     2281 aatattaaag acaggactcc ataaatattt tctctggatg gaattgttaa ggtaaaaata
     2341 attgatagag tataaatggg acttgttccg atgtcgccga atactatacc cattgcttta
     2401 attatatcct tagcaggatt tgagtgtgca cttttcatgt attaactctt ttgcatttct
     2461 ttaactgcaa aaagctgttt acacctttcg atttttaatg tgcgtaaaaa tatggtataa
     2521 cttatttcaa tcacaatatt tgattagata ttaaataatt ttctttttta acatttactt
     2581 caattttgaa taactaatct ttattttctg gcaacaattt ttatctcatt aaaacctcgt
     2641 tatgaaaaac aagaaatttg taattattga tgcaatggca cttgcctatc gcgcctactt
     2701 tgcttttatc agcagaccat taactaacag caaaggtgaa tcgacttctg ctgtttatgg
     2761 ttttctaaat cagctaatca aagtttttga agatcacaaa cctgattacc tggctgttgc
     2821 ttttgattcc aaagaaaaaa ctttcagaca cgaaaagtat gaagcttata aatcttcgcg
     2881 cgatgcaatg ccggatgata tgattccgca gctacaaaga ataaaagata ttgtaatcgc
     2941 attgaacatt ccattgtata ttttaccacg ctacgaagct gatgatattg ttggaacggc
     3001 tgtaaaaaag gctgaagaat taggaatgct ttcttacgcg ataacacctg ataaagatta
     3061 ctttcaatta attacagata aaaccaatgt tgtaagaccc ggtaaaaagt ccgatgaagc
     3121 aattctatac gacaagcaaa aagttattga tgaacttggt ttcgaaccaa aatatatggt
     3181 tgattatctt gctttagtcg gggacagcag tgatgacatt ccaggtgttg cagggatagg
     3241 tccaaaaggc gcagtgccat tgatccaaaa gtatggaacg attgaaaata tttacagaca
     3301 tattgatgag atagaaaaag ccggaatcaa aaagaaattg
//
LOCUS       DASWBY010000647         8004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_551258, whole genome shotgun sequence.
ACCESSION   DASWBY010000647 DASWBY010000000
VERSION     DASWBY010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551258"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2944)
                     /locus_tag="VF870_11820"
     CDS             complement(<1..2944)
                     /locus_tag="VF870_11820"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEX9252924.1"
                     /translation="MFILFVGDLKSQQVYINELMSSNEVTIPDEDGDYSDWIEIFNQE
                     ENNLDLSGYGLSDDVSVPFKWVFPSLTFEPKDHLLIFASDKNRTEYIKHWETVIDWGD
                     VWKYKIGTSEPPAAWKTLGFDDQLWSSGPSGFGYGDNDDSTIVPNNTNSVYIRKVFSV
                     ADVNNVKMAVLHVDYDDAFVAYLNGIEIARANIGTVNIPPAYNESAASFTEPLIVYGG
                     KPNSYIIQNYQSLLQNGDNVLAIQVHNYGTGSSDLTLIPFLSFGMDVVPLNPNGVNPL
                     LDLPNKFLHTNFKLSSSGETLILTNPQNVTVDEVTFNTIGPDISFGRQPDGSSNWFLF
                     TDATPGDSNSTQGFTGTAAEPIISISGGFYSSPLSVTVSPASVNDNIYYTLDGSEPKE
                     TSTPYSIPIQINSTKVLRVKTFGAGKLPSKTLTNSYFINFSTGLTVVSLSTDPGNLFD
                     EEYGMYAMGDSAETSFPYFGANFWQDWERPVHIELFETDGSKGFGIDMGAKIFGGWSR
                     GNAQKSLALFARGQYGFSTLNYKLFNELPFTEYESFVLRNSGNDWGSTMFRDALMTSL
                     VDGLDIDKQDYRPALLFINGEYWGIQNIREKVNEHFLAQHHSVNPDSVDILEYFGEVV
                     QGDSTEYLELYNFIENNSMAVPSNYEYVKSKMEVDNFIKYFISEIYFDNQDWPGSNIK
                     YWEKNTDGRWRWIMFDTDFGYGIWDSNAYQNNTLQFALEPNGPDWPNPPWSTLFLRKL
                     LENNSFKNDFINYFADLSNTNFKPVNVVNKINSMASVIEPEITRHAAKWNQFTYNQWL
                     TNVQNMRYFASHRPDYLRAFYLQKFNLTGTSVVNISISDTSMGSVKLNSLDITLPNWS
                     GIYFLNVPLNVIAQPREGYRFIGWSGSSNSSDDSLIIILNGTINLNAVFEVDSNYSLP
                     DIVINEISYNPSASFNTEDWIEIYNNGESDLDISGWIYKDSDDSHSFVIPQGTVLASE
                     EYLVMCVDT"
     gene            complement(3172..5421)
                     /locus_tag="VF870_11825"
     CDS             complement(3172..5421)
                     /locus_tag="VF870_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9252925.1"
                     /translation="MKLIVKFFSFVNLIILILNTNICAQIYTTTKVDAKVKELVSKMT
                     LEEKIGQMTQVAIQTVSKVQGTKDQMHQLDFAKLEDAIKKYHVGSILNVYDIANTADY
                     WHEIINKIQDIAVKETRLGIPVLYGIDAIHGATYTKGSTLFPQAISMAATWNPEIAEQ
                     SGEITSFETKASGIDWNFYPVMDIGRQPLWSRLWETFGEDVYLASQMGSSYIKGAQGS
                     DISAPNKLATCLKHYVGYSFPINGLDRTPAWISERMMREYFLPPFEAGILAGAPSIMV
                     NSSEVDGIPGHANYHLLTEVLRNELNFKGLVVSDWQDIKRLHTRDKVASTPKEAVRIA
                     VMAGVDMSMVPDDFSFYNYLIELVNEGTVPMWRIDEAVSRILAVKMQIGLFDNPYPNK
                     ELLKDFANDKFTSINLNAARESIILTKNEESILPLSKKTKVLVTGPTANLLSALNGGW
                     TITWQGNEESAYPQEKNTVLEAIQNKISKENVNYVEGCSFDKDINTSKAVSEAMNSDV
                     IVICLGEPAYCETPGSIFDLNLYKAQLELANKLIETGKPVILVMLEGRPRIITDIAQN
                     AKGILLGFLPGMEGGNAIADVLFGDVNPSGKLPITYPKTSNGITLYDYKPIEKFDGND
                     YQPMWPFGFGLSYTTFEYSNLKISSQEITENDELIVKIDVKNNGKKVGKEVVQLYLTD
                     MYGSVTRPNKQLKAFKKISLNPGEVKTVEFNINRQDLSFIGQENKRIVEPGDFRVTIQ
                     NQTADFTLK"
     gene            complement(5558..7396)
                     /locus_tag="VF870_11830"
     CDS             complement(5558..7396)
                     /locus_tag="VF870_11830"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEX9252926.1"
                     /translation="MNQVFMKIKIIFFILLISLSAQINVLSREFKGAEYRTKLSYTYG
                     RFEVRMKSAYRDGMLSSFFTYYDGGGGIGNWNEIDIEIMGRYFDNVQFNTITPNQTNH
                     VAHKPMQNSPHQDYHTYAFEWTPEYVAWFVDGNEVLKQTGEHIHTLTLPQKIMMNIWN
                     PAFENWAGVFNPDALPAFAYYDWVSYYAYTPGSGTYGTGNNFSHDWTDNFDAWDTDRW
                     EKATHTFNGNNCDFIQANAVFNDGKLVLCLTDSIYTGYVDAKPPALVWARASTDNKVL
                     VMFSEELNQEAAENISNYVITGAAVNSATLQPDLKSVELSVTGLVIPSIKNLVVMSMK
                     DNEVVPNTMSPKLTQIIMPQPLTFPIKINCAGSTALGYLQDTEWTKNTEFGFLDGNST
                     NYPTSLPISGTDEDIIFQSEKYGMVTYKVRLTNGNYNVKLMFAENYFDQAGSRVFDVY
                     IEQNRVIENLDIYSLAGKNAAYVSEITNVEVNDGVLDIQFGDKINNALICGISITPAA
                     TGLFDDENPIELKSFKVEQNYPNPFNGKTVINYSLTSPDNLSFELYNVLGEEIFFKDL
                     GYKQSGTYKFQLDTASLSDSPLTSGIYFYVFTGMNTREIRKFVLLN"
     gene            complement(7449..>8004)
                     /locus_tag="VF870_11835"
     CDS             complement(7449..>8004)
                     /locus_tag="VF870_11835"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9252927.1"
                     /translation="KFIYESIWQMSANAFALDLGVQYVTPFDDAVLAMSISNFGTTMQ
                     LLGNSNLVLHDLDPENSGNNGDIPAYLETNSWALPLTFRVGIAYDPIKMDMHRWTVAL
                     DAMHPSDDYESINVGTEYAYNDFLFLRAGYKALLLDDSEESFSFGFGVKQLLIGNVAF
                     MIDYAYQDFGRFSDIQKFTLTVTF"
BASE COUNT         2555 a         1541 c         1250 g         2658 t
ORIGIN      
        1 aggtatcaac gcacatgacc agatattcct cagaagccag aactgttccc tgaggaatta
       61 caaaactgtg agagtcatca gaatccttat agatccagcc tgaaatatct aaatcacttt
      121 caccattgtt ataaatttca atccaatctt ctgtattaaa cgacgcggaa ggattataac
      181 tgatttcatt tatcacgata tcaggtaaac tatagtttga atcaacttca aatacagcat
      241 taagatttat tgtaccattt aaaattatta taagtgagtc atcacttgag ttgctggatc
      301 cactccagcc aataaaccta taaccctctc taggttgagc aataacattt agaggaacat
      361 ttagaaaata aattccgctc caatttggta aagtgatatc caaagaattt aactttacac
      421 tgcccattga agtatcagag atagaaatat ttacgactga tgtaccagtc aaattaaatt
      481 tttgcagata aaaagctctt agataatccg gacgatgact cgcaaagtat ctcatattct
      541 gcacgtttgt aagccactga ttatatgtaa actgattcca ctttgccgca tgccttgtaa
      601 tttccggttc tattacggat gccattgaat ttattttatt gacgacattt accggtttaa
      661 agtttgtatt tgaaagatca gcaaagtaat taataaaatc atttttaaat gaattgtttt
      721 ctaatagttt acgtaggaat aatgttgacc atggtggatt tggccagtct ggtccatttg
      781 gttctaaagc aaattgaaga gtattattct gataggcatt tgagtcccat attccgtaac
      841 caaaatcagt atcaaacatt atccatcgcc atcttccatc ggtattcttt tcccagtatt
      901 taatattact tcccggccaa tcctggttat caaaataaat ttcagatatg aaatatttaa
      961 taaaattatc tacttccatt tttgatttta cgtattcata attcgaggga actgccatac
     1021 tattgttttc aatgaagttg tatagctcca aatattctgt gctgtcaccc tgtacaacct
     1081 ctccaaaata ttcaagtata tcaacactat ccggatttac actatgatgc tgcgccagaa
     1141 aatgttcatt aactttttca cgaatatttt gaattcccca atattctcca ttaataaata
     1201 acaatgccgg acgataatct tgtttatcga tatcaagtcc atcgactaaa cttgtcatca
     1261 gtgcatcgcg aaacattgta gaaccccaat cattccctga atttcgtaat acaaaagatt
     1321 catattccgt gaatgggagt tcattaaata atttatagtt caacgtactg aaaccatatt
     1381 gtccccgggc aaatagagct aatgattttt gagcgttacc tctgctccat ccaccaaata
     1441 tttttgcacc catatcaata ccgaatcctt ttgaaccatc tgtttcaaaa agttcaatat
     1501 gaactggtct ctcccaatcc tgccagaagt ttgccccaaa atatgggaat gaagtttctg
     1561 cactatctcc cattgcatac attccatatt cttcatcaaa taaattccct ggatcagtgg
     1621 aaagcgaaac aacggtgagc ccggttgaaa aatttataaa gtaagaattg gtcaatgttt
     1681 tactcggtaa tttcccggct ccaaatgttt ttacccttaa tactttcgtc gaattaattt
     1741 gaattggaat ggaatatgga gttgatgttt cctttggctc agatccatct aaggtataat
     1801 aaatattatc attaactgaa gcgggtgaaa ctgtaactga taatggtgaa gaatagaagc
     1861 caccgctaat agaaattatg ggttcagcag ccgttcctgt aaaaccttga gttgagttac
     1921 tatcaccggg tgttgcatca gtaaataaaa accaattact gctcccatcg ggttgtctgc
     1981 cgaaagaaat atcaggtcca attgtattaa aagtaacttc atcaacagta acattttgtg
     2041 ggtttgttaa tattaatgtc tctcctgatg aacttaattt aaaattcgtg tgaagaaatt
     2101 tatttggtaa atcgagcagc gggttaactc catttggatt tagtggaact acatccattc
     2161 cgaaagataa aaatggaatt aaagtaagat cagaggagcc cgttccataa ttgtgtacct
     2221 gaatcgctag tacattatct ccattctgta aaagggattg ataattttgg atgatataag
     2281 aatttggttt accgccataa acaatcaatg gttctgtaaa agatgctgca gattcgttat
     2341 aggcaggtgg gatattaaca gttccaatat tcgccctggc tatttctatt ccatttagat
     2401 atgctacgaa agcgtcatca taatcaacgt gaagaactgc cattttaaca ttattaacat
     2461 cagctacaga aaatactttc cttatataaa cagaattagt attgtttgga acaattgtag
     2521 aatcatcatt atctccataa ccaaagccgc ttggaccaga agaccaaagt tgatcatcaa
     2581 atcctaaagt tttccaggcg gccggcggct cgcttgttcc tattttatat ttccatacat
     2641 cgccccagtc aatcacagtt tcccaatgct taatatattc agtcctgttt ttatcacttg
     2701 caaaaatcag caaatgatcc tttggttcga aagttaatga tggaaagacc catttaaatg
     2761 gaacacttac atcatcggat aatccgtaac ctgagagatc caaattattt tcttcctgat
     2821 taaaaatttc aatccaatca gaataatccc catcctcatc cggaattgtc acttcatttg
     2881 aggacatcaa ttcatttata taaacttgct gagattttaa gtctccaaca aagagtataa
     2941 acactaaaaa aaatatcggc agcaaattta ttttaatacg aaacacttta atctttcaaa
     3001 tcattattga cgtaaataaa aaaggaagat acacaatgct ttatgctgtg atcttccctc
     3061 aaaaataata ttagtttatt ctttagattc ctgaaacatt atgatgtact ataccagaat
     3121 tttgttttga aaaaatattt cattcaaaat aaaattctta aaaccctgat tctattttaa
     3181 tgtgaaatca gcagtttgat tttggatagt caccctaaaa tcacccggtt caacaattct
     3241 tttattttcc tgcccgataa aagataggtc ctgcctattt atattaaact caactgtctt
     3301 aacttcgccc ggattcaatg atattttttt aaatgcttta agctgtttat ttggccttgt
     3361 tacagaacca tacatatcgg tcaaataaag ttgaacaact tctttgccaa cctttttgcc
     3421 attattttta acatcaattt taacaatcag ctcatcattc tctgtgattt cttgagaact
     3481 tatcttaaga ttggaatatt caaatgttgt atagcttaaa ccgaaaccga acggccacat
     3541 tggctgataa tcattcccat caaatttttc aattggttta taatcgtaca atgtgattcc
     3601 gtttgaagtt ttaggatatg taataggaag tttgccgctt ggattaacat caccaaatag
     3661 aacatcagca attgcgttgc caccctccat tccgggtaaa aatccgagta aaattccttt
     3721 agcattttgt gcgatgtctg tgattatcct tggccttcct tctaacatta caagtatgac
     3781 tggtttaccg gtttcaataa gtttatttgc taattctaac tgcgctttgt acaaattaag
     3841 atcaaagata ctacctggtg tttcgcaata ggcaggttca ccaaggcaaa ttacaataac
     3901 atcagagttc atagcttcac taaccgcttt gctggtatta atatctttat caaagctgca
     3961 gccttcaaca taatttacat tttctttact tattttattt tggattgctt caagcactgt
     4021 atttttttct tgcggataag cagattcttc attcccctgc caggtgattg tccatccgcc
     4081 atttaatgct gaaagtaaat ttgcagtagg tccggttaca agcactttag tttttttaga
     4141 aagaggcaaa atactttctt cattctttgt tagtataata gattctcttg cagcatttaa
     4201 attgatagat gtaaacttat cgttagcaaa atcttttaag agttctttat taggatatgg
     4261 gttatcaaaa agaccaatct gcattttaac ggcaagtatt ctgcttacgg cttcatcaat
     4321 tctccacatc gggactgtac cttcatttac aagttctata agataattgt agaagctaaa
     4381 atcatcgggt accatgctca tatcaacacc ggccattaca gctattctta ctgcttcttt
     4441 aggcgttgat gcaactttat ccctggtatg taatctttta atatcctgcc aatctgaaac
     4501 aactaaacct ttgaagttta attcatttct taaaacttct gtaagtaaat gataatttgc
     4561 atgaccggga attccatcta cttctgatga gttgaccatg attgagggag ccccggctaa
     4621 tatgcccgct tcgaagggag gaagaaaata ctcccgcatc attctttcag aaatccaggc
     4681 aggtgttcta tccagcccgt tgattggaaa gctgtatcct acataatgtt ttaaacaagt
     4741 tgcaagctta ttcggagcac taatatcgct gccctgtgca ccttttatat aagaagatcc
     4801 catttgtgaa gccagataaa catcttcacc aaaagtttcc cataatctgc tccaaagcgg
     4861 ctgtcttcca atatccataa ctggataaaa attccagtca attccggatg ctttcgtttc
     4921 gaatgaagtt atttctccgc tctgctcggc aatttctgga ttccaggtag cagccatact
     4981 aattgcttgt ggaaagaggg ttgatccttt agtatatgtt gcaccatgta ttgcatcaat
     5041 tccatacagc actggtatgc ccagtcgagt ttctttaacc gcaatatctt gaattttatt
     5101 aattatttcg tgccagtaat ccgcagtatt tgcgatatca taaacattta agattgaacc
     5161 aacgtgatat ttttttattg catcctcaag cttggcaaaa tctaattgat gcatttgatc
     5221 ttttgttccc tgaacttttg atactgtctg aattgcaact tgagtcatct gtccaatttt
     5281 ttcttcaagt gtcattttag aaacaagttc ttttacctta gcatcaactt tggttgtagt
     5341 ataaatctga gcacaaatat tagtattcag aattaagatt ataagattta caaacgagaa
     5401 aaacttaact attaatttca taaaaattcc tgcatttaat ttttttattt agtgatattt
     5461 ttttttagaa tgatagaaag agattttaca aggaggaata catcaatttg atttaaagaa
     5521 aatgataata tcaaagattt acaaatcata attcaattta atttagcagt acaaattttc
     5581 gtatttctct tgtgttcata ccagtgaata cataaaaata aattcctgat gtaagcggac
     5641 tatcagataa tgatgcggta tcgagttgaa atttatacgt cccggattgt ttataaccta
     5701 aatccttaaa aaaaatttct tcacctaaaa cattgtataa ctcaaaactt aaattatccg
     5761 gagatgttaa tgaataatta atgactgtct ttccattaaa cgggtttgga taattctgtt
     5821 caactttaaa tgattttagt tcaataggat tttcatcatc aaataaacct gttgctgcag
     5881 gagtaatact tattccgcat attaatgcgt tattaatttt atcaccaaat tgaatatcca
     5941 gcacaccatc atttacctca acattagtaa tttcactaac gtatgcagca ttttttcccg
     6001 caagcgaata aatatctaaa ttttcaatca ctctattttg ctcaatataa acatcaaata
     6061 ccctgctgcc ggcctgatcg aaataatttt cggcaaacat taactttaca ttataattgc
     6121 cgtttgttaa tcttacttta taggtaacca tcccatattt ttcagattga aaaattatat
     6181 cttcatccgt tccgcttatc ggtaaagaag taggataatt agtagaattt ccatcaagaa
     6241 aaccaaactc tgtattcttt gtccattcgg tatcctggag ataacctagt gcggtactac
     6301 cggcacaatt aatttttatt ggaaatgtta atggctgcgg cataattatc tgagtaagtt
     6361 ttgggctcat cgtattcgga acaacttcat tatctttcat cgacatcaca acaagatttt
     6421 taatagatgg gataactaaa cccgtaacag aaagctcaac actttttaaa tccggctgaa
     6481 gcgttgcact attaactgct gcgccggtaa ttacatagtt ggaaatattc tcagctgctt
     6541 cctgattaag ttcctccgaa aacattacaa gcactttatt atctgttgaa gcccgtgccc
     6601 aaactaatgc cggcggcttc gcatcaacat aaccggtata gatgctgtca gttaagcaca
     6661 aaacaagttt accgtcatta aaaactgcat tagcttgaat aaaatcacaa ttatttccat
     6721 tgaaggtatg tgttgccttc tcccatcgat ctgtgtccca cgcatcaaaa ttgtccgtcc
     6781 aatcatgcga aaaattattt ccagttccat atgtgccgga accaggagtg taggcataat
     6841 aacttaccca atcatagtaa gcaaatgccg gtaatgcatc gggattaaaa actcctgccc
     6901 aattttcaaa agcaggattc cagatattca tcattatctt ttgaggcaag gtaagagtat
     6961 gaatatgttc gccagtttgt tttaagactt cattcccatc tacaaaccat gcaacatatt
     7021 ctggcgtcca ttcaaaagca taagtatgat aatcctgatg aggagaattt tgcattggtt
     7081 tatgtgccac gtgattagtt tgatttgggg taattgtatt gaactgtaca ttatcaaaat
     7141 agcgccccat aatttcaata tctatttcat tccagttgcc tattccgcct ccgccatcat
     7201 agtaggtaaa aaaagaggaa agcatcccgt cacggtaagc tgatttcatt ctaacttcaa
     7261 atcttccgta agtgtacgaa agttttgttc tgtactctgc gcctttaaat tctctactta
     7321 gaacatttat ctgcgcagat aaactaatta gtaaaatgaa aaatataatt tttattttca
     7381 taaaaacttg attcataaaa gattaaagcc ctcctcaaaa aggaataaag aagagggctt
     7441 cattatggtc aaaaagttac tgtaagagta aacttttgaa tatccgaaaa tctaccgaag
     7501 tcttgataag cataatcaat cataaatgca acattgccaa tgagtaattg cttaacacca
     7561 aatccaaagg aaaatgattc ttctgaatca tcgagtaaca atgctttgta tccggctctt
     7621 aagaacaaga aatcattata agcatattcg gttccaacat ttatactttc gtagtcatcg
     7681 ctcgggtgca ttgcatccag tgctactgtc catctatgca tatccatttt tataggatca
     7741 tatgcaatac caactctaaa agttaaaggc agtgcccatg aatttgtttc aagatacgca
     7801 gggatatcgc cattgttgcc actattttcc ggatcgagat catgcaatac cagatttgag
     7861 tttcccaaca attgcatagt agttccgaag tttgaaattg acatagcaag aactgcatca
     7921 tcaaatggag tgacatattg tacaccaaga tctaaagcaa atgcattagc actcatctgc
     7981 cagatacttt cgtagatgaa ttta
//
LOCUS       DASWBY010000648         5213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_29573, whole genome shotgun sequence.
ACCESSION   DASWBY010000648 DASWBY010000000
VERSION     DASWBY010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29573"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1106
                     /locus_tag="VF870_11840"
     CDS             87..1106
                     /locus_tag="VF870_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252928.1"
                     /translation="MMIKLFMRYVMLSVLCLFTFISCSDENNNDPDADSLNKEIANAH
                     LTEFSGFYFVGSFDGRPSIYRYDRSSDKFKIFWNSNEERVIDLLISPDNSSGYFITKR
                     KQRLKSSQPAIEKGRLYRLDFEVKKVEPISQLEEGIQIIPFWMDNDRFTLVINSIDKT
                     IASYINKNIQVYNRFGKLLSDNTEVYDLTKDGYPITKLPPVRYKSPNEMFLVFEKNDS
                     IIVRQQKSQKEMNTGLSSGKILRIEWAENNKHLIMLMQSKDEEKPADNKAIKTHLAIY
                     DLQQKKVVKKFDTVGYSPFVLIGDFLIFDNGIGKDSYIEIFKLDSLSNFKTIKINGGC
                     GLRNI"
     gene            1123..2988
                     /locus_tag="VF870_11845"
     CDS             1123..2988
                     /locus_tag="VF870_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9252929.1"
                     /translation="MNSYYRILKYTKPYWRHILLTFVFTILFAILNGVSVYLTIPLLD
                     TLFQESASKQTTVQSTNIEKTSSIVPDWINNLKEDVVRSFNEFVFRGDKPEVLIKICF
                     LILIAFLLKNVFGYLQGYFMAFVEYASMKDLRDDTYKHLHKLPMGYFKSERVGNIISR
                     FTNDVNVIQLSISTTFSNLIKEPLTIVVFLGIAISISWQLSLFAFIIVPIASLIIAWI
                     GNKLKKQTIILQTKLADITSILQETISGVKIVKAFGMEEFENQRFMNETKSYFKLVLK
                     TVRIRNLSSPITEILSVIIGAVIIYYGGILVLVDKTLNASQFLGFLFAIFQMMPPIKE
                     LGSVNNRIQEASAAADRIFEITDIEPHIKNIPNAIQLTDFKNEIVFKNVSFQYEDSDV
                     KVLDNLNFSVKRGEIIALVGPSGGGKSTLADLIPRFYDPTDGQIFIDGKNIKDVTIES
                     LRSKMGIVTQETFLFNTNVAENIAYGLTDYPMEKIIDAAKTANAHVFINEMPQGYNTV
                     IGERGVKLSGGQRQRLTIARALLKNPEIMIFDEATSALDNESEILVQEAIERLMLNRT
                     TFVIAHRLSTIRNATNILVLDRGRIIQQGTHDKLIAEEKGLYKKLYEMQFRDNEN"
     gene            2978..4171
                     /locus_tag="VF870_11850"
     CDS             2978..4171
                     /locus_tag="VF870_11850"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9252930.1"
                     /translation="MKTEEKMLKSRFNSKIIFSLIVILAISFVLSLFVMQLAAGILFI
                     LWLFEKHQERKKAYDQIIAFIIIFGLVRLISIFLSEYPHTSYESLYKEGLFYTSAAAI
                     PFYFKTLNKKLILNLMLAFIIGAAVMSLVGSIRFFINDVERAQAFTSSYAVFSSYLLA
                     AFSVALFFPKKIKSNSELLFWSVIYIIILTGIVTSLGRANIAITLLMLLTAIVLKQIP
                     LKQIIILGLFFVIGVSVYLAKPAASIEQRVENITQLSDRDIILQGAKEISLNHPVIGY
                     GPRTFQEIFPFRERFADKGIGGWHNDFLQIYFESGVVGLISFVALLWVIIMMSFNQLR
                     NKKTDAELKSLSASVLASVIALLLSSLFAGFITSVVLSIVFVFLISFISRIDFEISIA
                     QETSN"
     gene            complement(4164..4622)
                     /locus_tag="VF870_11855"
     CDS             complement(4164..4622)
                     /locus_tag="VF870_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9252931.1"
                     /translation="MNTNLAKIFLVIVVFSSVSFAQSKGFGLGILLGQPSAISAKLWT
                     TPSTAFDFGLGYSFESQNRVHIHADYLFHVKNIFNTTENISLYYGPGARMSFHENDDS
                     RLGVRFDVGLVWIPRNAPIDVFIELAPILDIIPETSFAMNGGIGVRYFFN"
     gene            4776..>5213
                     /locus_tag="VF870_11860"
     CDS             4776..>5213
                     /locus_tag="VF870_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX9252932.1"
                     /translation="MNSEIYTKAKDYSTELNSSNEAAIILAAGHWKRIKSQTSKMLHR
                     IWEIPTVERVYNSCRLGIPNINTVVVVGIKALDVMDVIGKRESNKFAFQEKQNGTGHA
                     VQVGLKEIEKDFNDGIVYVLPGDMGLLDDSSMIKFREDFISSKK"
BASE COUNT         1816 a          777 c          905 g         1715 t
ORIGIN      
        1 gatatttcta caacccaaat ttatacacat attgatcgtg attacattaa acaaattcat
       61 aaacaatatc atccaagagg ttagctatga tgattaaatt atttatgaga tatgttatgc
      121 tttcagtttt gtgtttattt acatttatct cctgcagcga tgaaaacaac aatgatcctg
      181 atgcagatag tcttaataaa gaaattgcaa acgcacacct tactgaattt tcgggttttt
      241 attttgtagg cagctttgat ggcaggcctt cgatttacag atatgatcgg agttcagata
      301 aattcaaaat attttggaac tcaaatgagg aaagagtaat agacctcctg atatctccgg
      361 ataattcttc aggttatttt ataactaaga ggaagcaaag attaaaatca tcccagccag
      421 ccattgaaaa aggaagatta tatcgcctgg attttgaagt aaaaaaagtg gaaccaattt
      481 cgcagttaga agaaggtatt caaatcattc ctttctggat ggataatgat agatttacac
      541 tggttattaa ctcaatagat aaaacaatag catcatacat aaacaaaaat atacaggtct
      601 ataatcgttt tggtaaactg ctttctgata acaccgaagt ttatgattta acaaaggacg
      661 gttacccgat tacaaaactt ccgcctgtaa gatataaatc accaaatgaa atgtttttgg
      721 tttttgaaaa gaatgattca attattgtac gtcagcagaa atctcaaaag gaaatgaata
      781 ccggactatc atccggtaaa atactccgga tagaatgggc tgaaaacaat aaacatctga
      841 ttatgcttat gcaatccaaa gatgaagaaa aaccagcgga taataaagca attaaaactc
      901 atttagcaat ctatgatctt cagcagaaga aagtggttaa aaagtttgat acagttggtt
      961 acagcccctt tgtgctgatt ggcgattttc tgatatttga caacgggatt ggtaaagatt
     1021 catatattga aatatttaaa cttgattctt tatccaattt taaaacaatc aaaataaacg
     1081 gcggctgcgg tcttcgtaat atttaaaaca taaagagata atttgaattc atattacaga
     1141 atacttaagt atacaaaacc gtattggcgg cacatcttat taacatttgt gttcacaatc
     1201 ttatttgcaa tactaaatgg tgtgtcagtc tatcttacca tacctttgct cgatacatta
     1261 tttcaggaat cagcctcaaa acaaactact gtgcaatcaa ctaatattga aaaaacttcc
     1321 agcatagttc cggattggat aaacaattta aaagaagatg ttgtaagatc ttttaatgaa
     1381 tttgttttta gaggtgataa acctgaagtg cttattaaga tttgcttttt aatactcatc
     1441 gcattcttgc tgaaaaatgt ttttggttac ctgcagggat actttatggc ttttgttgaa
     1501 tatgcatcta tgaaggattt acgtgatgac acttataaac atttgcacaa attgccaatg
     1561 ggatatttta aatcagaaag agttggaaat attatttcaa gatttacaaa tgatgttaat
     1621 gttattcagc taagtatatc cacaacattt tcaaatctta taaaagaacc gcttactatt
     1681 gttgtttttc ttggtattgc aatcagcata agctggcagc tttctctgtt tgcttttatt
     1741 attgttccaa tagcaagttt aataattgca tggatcggta ataaactaaa aaaacagact
     1801 ataattcttc agactaaact tgcagacata acaagcatac tacaggaaac tatttcaggc
     1861 gttaagattg ttaaggcatt tggtatggag gaatttgaga atcaaaggtt tatgaatgaa
     1921 actaagagtt attttaaatt agttcttaag acagttagaa taagaaatct ttcatcgccg
     1981 attacagaaa tattaagtgt aataattgga gcagttataa tttattacgg cggaatactt
     2041 gtgcttgttg ataaaacact taatgcaagt cagtttttag gatttctatt tgcaatattt
     2101 cagatgatgc ccccgattaa agagcttgga agtgttaata atagaattca ggaagcaagt
     2161 gccgccgccg atagaatttt tgaaattaca gatattgaac ctcatataaa aaatattccg
     2221 aatgcaatac agttaacgga ttttaaaaat gaaatagtat ttaaaaatgt atcgttccag
     2281 tatgaagatt ctgatgtaaa ggttttagat aatctaaact ttagtgttaa acgcggagaa
     2341 attattgcgc ttgttggtcc aagcggcgga ggtaaatcca cccttgcaga tttgattcca
     2401 agattttacg atccaaccga tggacaaatt tttatcgatg gaaaaaatat taaagatgtt
     2461 accatagaat cattgcgctc aaaaatggga atagtaacgc aagaaacatt tttattcaac
     2521 acaaacgttg cagaaaatat tgcttatggt ttaacagatt atccaatgga aaaaattatt
     2581 gatgctgcaa aaactgcaaa cgctcacgtt tttataaatg agatgccgca gggttacaat
     2641 actgtaattg gagaaagagg agttaaactt tcgggaggac aaagacaacg cttaaccatt
     2701 gctcgtgcat tattgaagaa tcctgaaata atgatttttg atgaagctac atcagcatta
     2761 gacaatgaat ctgaaattct tgtacaggaa gcaattgaac gactgatgct gaacaggact
     2821 acctttgtaa ttgctcatcg tttaagtaca ataagaaatg caacaaatat tttggtcttg
     2881 gatagaggtc gaatcatcca gcaaggaaca cacgataaat tgattgctga agaaaaagga
     2941 ctgtataaaa aactttacga aatgcagttc agagataatg aaaactgaag agaagatgtt
     3001 aaaaagtcgt tttaattcta aaattatttt ttcattaatt gtaattcttg caatcagttt
     3061 tgtcttatct ctttttgtta tgcaattagc agcagggatt ttatttatcc tctggttatt
     3121 tgaaaagcac caggaaagaa aaaaagcata cgatcaaata attgcattca taattatttt
     3181 tggattagtg cgactaatct caattttttt atcagaatat cctcacacca gttatgaatc
     3241 attatataaa gaaggattgt tttatacttc tgctgcagca atcccatttt attttaaaac
     3301 tctgaataaa aaattaatat taaacttaat gttggcattt ataatcggtg ccgcagttat
     3361 gtctttagtg gggagtataa gattttttat aaatgatgtt gaacgtgccc aggcttttac
     3421 ttcaagctat gcggtttttt ccagctactt actagctgca ttttctgttg ctttgttttt
     3481 tcctaaaaag ataaaaagca attctgaatt attattctgg tctgtcattt atataatcat
     3541 cttgaccgga atagttactt cattaggcag ggcaaatatt gcaattactt tattgatgtt
     3601 gttaacagct attgtgctaa aacaaattcc cttaaagcaa ataattattt taggattatt
     3661 tttcgtaatc ggagtttctg tttacttagc taaaccagct gcatcgattg aacaaagagt
     3721 agaaaatatt actcagcttt cagatagaga tataatcttg cagggagcta aagaaattag
     3781 tttgaatcat ccggttattg gatatggacc aagaactttt caagaaatat ttccgtttcg
     3841 ggaaagattt gccgataagg gaattggcgg atggcataat gattttttac aaatctattt
     3901 tgagagcggt gttgttggat tgatttcttt tgtggcttta ttatgggtga taataatgat
     3961 gtcatttaat cagcttagaa ataaaaaaac agatgcagaa ttaaaatcac tttctgcatc
     4021 tgttctagct tcagtaatag cacttttgct ttcttcacta tttgcgggat ttataacatc
     4081 agtagtttta tctatagtat tcgttttcct aatttcattt atttcaagaa tagactttga
     4141 aatatctatt gctcaggaaa cttctaatta aaaaaatacc gcacacctat tccgccattc
     4201 attgcaaaac tggtttctgg tattatatcc agtatcgggg caagttcgat aaatacatct
     4261 attggagcgt ttcgtggaat ccaaaccaga ccgacatcaa atctaactcc caatcttgaa
     4321 tcatcatttt catgaaaact cattctcgca ccggggccgt agtacagcga aatgttttcg
     4381 gttgtgttaa agatattttt tacgtggaat aaatagtcgg cgtgtatatg aactctgttt
     4441 tggctttcaa atgaataacc taatccaaaa tcaaaagccg ttgagggtgt agtccataat
     4501 ttagcgctta ttgccgatgg ttgaccaagt aaaattccca atccaaaacc ttttgattga
     4561 gcaaatgata cacttgaaaa aacaactata accagaaaaa ttttagctaa attggtgttc
     4621 attaacaatc ctttttttta ttaaaattgc aggcaaattt aataattaca gctaaacaat
     4681 aaaatgtaaa ggtttggaat aggaaatggc tttaattaat ttttcagtaa taaaatgttc
     4741 aattaaaaag cagcaataaa aacatgaatg atactatgaa ctcagaaatt tataccaaag
     4801 caaaagacta ttctacggaa ttaaactctt caaatgaggc tgcaataatt cttgccgcgg
     4861 gacattggaa aagaattaaa tcacaaacat ctaaaatgct tcataggatt tgggagattc
     4921 ccactgttga acgggtttat aattcctgca gacttggaat tccaaatatc aacacagttg
     4981 ttgttgttgg cattaaagct cttgatgtta tggatgtaat cgggaaacgt gaatcaaata
     5041 aatttgcttt tcaggaaaag cagaatggta cgggacacgc tgttcaggtt ggattaaaag
     5101 aaattgaaaa agattttaat gatggaatag tttatgtact tcctggtgat atgggtctat
     5161 tagatgattc ttcaatgata aaattcagag aagattttat ttcatctaaa aaa
//
LOCUS       DASWBY010000649         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_551284, whole genome shotgun sequence.
ACCESSION   DASWBY010000649 DASWBY010000000
VERSION     DASWBY010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551284"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1558
                     /locus_tag="VF870_11865"
     CDS             119..1558
                     /locus_tag="VF870_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9252933.1"
                     /translation="MNQFTAEKLKSIVGAPNFFDSKEDKLVYSYDGTPVYQQLPEAVL
                     FPQDEEQISKILKLANQEKFNVVPRGSGTGLSGGSIPVENSVVIVMTRWNKILEVDAD
                     NLTAWVQPGVITGILQKEVEKLGLFYPPDPGSLNVCTIGGNVANNAGGLRGLKYGVTK
                     NYVLGIEMILPNGELLKTGGKNMKDVAGYNLRDFIIGSEGTLGIVTKILLKIIPKPTQ
                     AITLLIYFDKLVDAGKTVSDIISSHIVPSMMEFLDNTTINCVEDYTHIGLPRNSEAIL
                     LIEVDGRGSEVQEDAELIRKIAVKNNASALKGAADETEANILKTARRSAFSALARRMP
                     TTILEDATVPRSELPVMIEKVAKAANTFDVMIGNFGHAGDGNLHPTCLTDERDQKELE
                     KTHRAFDFIFNESIKLGGTITGEHGTGLAKKQFLEAVAGPAGVDMMKRVKSAIDQNNI
                     LNPGKIFTISPKCEGSMPTKREQIKDFHV"
BASE COUNT          552 a          262 c          321 g          456 t
ORIGIN      
        1 accgcttaaa attttttaga aagtttattt aattaatcca gatctaattt taatgagtac
       61 ttaaatacta aaaatttaga aaaatgagta actttgttct gtaaaaacag gaatatttat
      121 gaatcaattc actgcagaaa aacttaaatc gattgtaggt gccccaaact tttttgattc
      181 caaagaagat aaattagttt attcatacga tggcacacct gtttaccagc aactccctga
      241 agctgtgtta ttcccgcagg atgaggaaca aatttctaaa atcttaaaac ttgcaaacca
      301 ggaaaaattt aatgttgttc cccgcggctc aggcactggt ttaagcggcg gttccattcc
      361 cgttgaaaat tctgttgtaa ttgtaatgac aagatggaat aaaattcttg aagttgatgc
      421 agataatctt actgcttggg tgcagccggg cgttataacg ggaattctgc aaaaggaagt
      481 tgagaagctt ggattgtttt atccgcctga tcccggaagt ttaaatgttt gtacaatcgg
      541 cggtaatgtt gcaaacaatg caggaggctt aagaggactt aaatacggtg ttacaaaaaa
      601 ttatgtactt ggcatagaaa tgattcttcc aaacggcgaa cttctaaaga ccggcggaaa
      661 gaatatgaaa gatgttgcag gctataatct gcgtgatttt attatcggta gtgaaggaac
      721 tcttggtata gtaacaaaaa ttttacttaa aattattcca aaaccaactc aggcaattac
      781 acttctgatt tatttcgata agcttgttga tgcagggaaa actgtttccg atattatttc
      841 ttcacacatc gttccttcaa tgatggaatt tttagataac accacaatta attgtgtgga
      901 ggattatacc catattggtc tgccaagaaa tagtgaagca attttattaa tagaagtaga
      961 cggaagagga tctgaagttc aggaggatgc agagttgatt agaaaaatcg ctgtgaagaa
     1021 taatgcctca gcacttaaag gtgctgccga tgaaacagaa gcaaatattt taaagacagc
     1081 aaggcgaagc gctttcagtg cattagcacg cagaatgccc acaacaattt tagaagatgc
     1141 aaccgtacca agaagtgaac ttcctgtgat gattgaaaaa gttgctaagg ctgcaaacac
     1201 gtttgatgtt atgatcggaa attttggtca cgccggcgat ggtaatcttc atcccacttg
     1261 cttaacagac gaaagagatc aaaaagaact tgaaaaaaca catcgtgcat ttgattttat
     1321 ctttaatgaa tctattaaac taggtggtac aattacgggc gaacacggaa caggcttagc
     1381 taaaaaacaa tttctggagg cagttgccgg accagcaggt gttgatatga tgaagagggt
     1441 aaaatcagct attgatcaga ataacatctt aaatcccggc aaaatattta caatctctcc
     1501 taaatgtgag ggaagtatgc cgaccaaacg agaacaaata aaagattttc atgtctaagg
     1561 aaattcagta caaagaatta ttaaaaattc t
//
LOCUS       DASWBY010000650         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_551317, whole genome shotgun sequence.
ACCESSION   DASWBY010000650 DASWBY010000000
VERSION     DASWBY010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551317"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VF870_11870"
     CDS             <1..778
                     /locus_tag="VF870_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9252934.1"
                     /translation="SILPKLRKLENLGFHPNVIRVLSFNYVKQGLVLVTGVTGTGKST
                     TLDAIVDWHNSNVNAHIVTIASPLEYVHRSKKCIIRQREVGKDVLSFREGVVQALRQD
                     LDIMVIGEMRDEDTIIAALEVTDTGHKVFSTLHTSSTVESIDRIIAEVGESEQTRVRN
                     RLADVLTAVISQKLVPDSKGQLTMAKEVLIVTPNVRAAIKNNNTDEIYMMINQAADLG
                     MITMEHDLKQLYQNKIITYDTALKFANNKTLFKQITGHAL"
     gene            1132..2586
                     /locus_tag="VF870_11875"
     CDS             1132..2586
                     /locus_tag="VF870_11875"
                     /inference="COORDINATES: protein motif:HMM:NF017519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HEX9252935.1"
                     /translation="MKKLKLLFAAVIIIYLILLIPEPHSDKIEAAQKTQFVWDRDSIW
                     HSLEDSFVKAKQLRCAKLSNQIWKDLLHFSNTLSSIEKINNLEPDNTIFDSLLNNVFQ
                     YAPLIGACPDSSEKYINLNGKLRQIIKDKSKNWDPNEIVTRNTLYKLLYGSRAAVEEI
                     ILQADKKDIYKLSYDNDEPSATPSVTFLGVKIHSGDILLSRGGAPTSALISRGSDYPG
                     NFSHVALVYIDPKSKTASVIEAHIEVGIAIATLEEYMKDKKLRVMVLRLRSDLPALIA
                     DPMLPHKAAQYSLQQALSQHIPYDFEMNYKNSNKLFCSEVASSEYKKFGIYLWMGKST
                     ISSLGTAKLLAGFGVKYFETQEPSDLAYDPQLSVVAEWRDIETLYKDHVDNAVVDAIL
                     EWSDKRNEIKFDWYMLPVFRIVKLYSVILNQFEIAGPIPEGMSATSALRHEAFKSFHS
                     DIKNVVLNKAEKFKEKNGYTPPYWRLLEFARNEL"
BASE COUNT          963 a          443 c          493 g          835 t
ORIGIN      
        1 ttcaatttta ccaaaactta gaaaacttga aaacttgggt tttcacccga atgttatccg
       61 tgtactttca tttaattatg ttaaacaggg actggtatta gtaaccggtg ttaccggcac
      121 aggaaaatca acaacattag atgctattgt ggattggcat aatagcaatg tgaacgcaca
      181 tattgttact attgcttcac ctctggaata tgtgcatcgt tccaagaagt gtattatcag
      241 acaaagagaa gttggtaaag atgttctttc tttccgcgaa ggtgtagttc aggctttacg
      301 acaggatttg gatataatgg ttataggtga aatgagagat gaagacacaa ttattgctgc
      361 cttagaagtt actgatacag ggcacaaagt attttcaact ttgcacactt catcaactgt
      421 agaatctata gatagaatca tagcagaagt aggagaatcc gaacaaacga gagttcgaaa
      481 cagacttgca gatgtgctta cggctgtgat atcacaaaaa cttgttccgg attcaaaggg
      541 tcaacttacg atggctaagg aagtattaat tgttacgcca aacgtaagag ccgcaataaa
      601 gaataataac acagatgaaa tttatatgat gataaatcag gctgccgatt tgggtatgat
      661 tacaatggag cacgatctta aacaacttta tcaaaataaa attattactt acgatacagc
      721 attgaaattt gcaaataata aaactctctt taagcaaatt acaggtcatg ctttataaaa
      781 taattttctt gcccttctaa tttctcttaa agagcatatc tgataaaaat ttagccataa
      841 aaaaccctga taccgaagca tcagggttag cactaacgca aggacggggt taatccttaa
      901 tcgttaacag ctaaacaaaa ataatgaatt gtgtacttat acaaaatacg atggattaaa
      961 tatctggaat ctttacttac acagttataa gaacttatgg aacagtgatt tagacacacc
     1021 ttaaaaaaaa ctctacatat ttatagttat aggaagaatc aaacaagctc aatatatttc
     1081 ggttatatct gaacagtatt tttattttca gtaattccga aaaaagaatt gatgaaaaag
     1141 ctaaaacttt tatttgcagc tgtaattatt atctatctta ttttgctaat tcctgaaccg
     1201 cattcagata aaattgaagc agcacaaaaa actcaatttg tttgggacag agattcaatt
     1261 tggcattcac ttgaagatag ttttgtaaaa gcaaagcaac ttaggtgtgc aaaattgagc
     1321 aatcaaattt ggaaggatct acttcatttt tcaaatactc ttagttccat tgaaaaaata
     1381 aataatcttg aaccggacaa tacaatattt gattcattgt tgaataatgt atttcaatac
     1441 gctccgttaa ttggcgcctg ccctgactcc tcagaaaaat acatcaatct taacggaaag
     1501 ctaagacaaa ttataaagga taaatcaaaa aattgggatc caaatgaaat agtcacacga
     1561 aatactcttt ataagttgtt gtatgggagt agagccgccg ttgaggaaat tatactgcaa
     1621 gcagacaaaa aagatattta taaattatct tatgataatg atgaaccttc ggctactcca
     1681 tcagtgactt ttcttggggt taaaattcat agcggtgata ttttattatc aagaggtggt
     1741 gctccgactt ccgctctgat atcacgaggc agcgattatc ccggaaattt ttcgcatgtt
     1801 gcccttgtgt acattgatcc taaatcaaag actgcatctg ttatcgaagc acatattgaa
     1861 gttggaatag ctattgccac gcttgaagaa tatatgaaag ataaaaaatt acgcgtgatg
     1921 gtattaagac tccgaagtga tcttcccgca ttgattgcag atccaatgct tcctcacaaa
     1981 gcagcacaat attctcttca gcaggctttg agtcaacata tcccgtatga ttttgaaatg
     2041 aattacaaaa actcaaataa acttttctgt tcagaagttg catcatcaga atacaaaaag
     2101 tttggaattt acctatggat gggaaaatca actatttcat ctttaggaac tgcaaaacta
     2161 ctcgcgggat ttggagttaa atattttgaa acacaagaac catccgatct tgcgtacgat
     2221 cctcagcttt cggttgttgc agaatggcgg gatattgaaa cactttataa agatcatgtt
     2281 gataatgcgg tagttgatgc aatactcgaa tggagtgaca aaagaaatga aattaaattt
     2341 gattggtata tgcttccagt atttagaatt gtaaaactct atagtgttat tctcaatcaa
     2401 ttcgaaattg cgggtccaat tcccgaagga atgagtgcaa cttctgcatt acgacacgaa
     2461 gcatttaaat cgtttcatag tgatataaaa aatgttgttt tgaataaagc agaaaagttc
     2521 aaagaaaaaa atggctatac tccaccatat tggcgattgt tagaatttgc tagaaatgaa
     2581 ttatgactta tgtaataata aaaaagcttc ctttagggga agctttttgc ggagagggcg
     2641 agactgtttt cagtcactta aatatttaca tttggataaa aaatcgatac aattttgatt
     2701 cgggtcgcat aaaactcgca ataattcgta tgtt
//
LOCUS       DASWBY010000651         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_590934, whole genome shotgun sequence.
ACCESSION   DASWBY010000651 DASWBY010000000
VERSION     DASWBY010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590934"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /gene="trxB"
                     /locus_tag="VF870_11880"
     CDS             <1..949
                     /gene="trxB"
                     /locus_tag="VF870_11880"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX9252936.1"
                     /translation="HFDVIVIGAGAAGLTAGIYLSRAKVKTLVLNEGTVGGQMVLTHK
                     IANYPGVENISGYELARTMKTQAEKFGCTIKSNLKITAMDLKDEIKRVVVNTKEIYTS
                     DAVIIATGGRSRMIGAIGEGKFKGKGISYCATCDGDFFQDKEIIVVGGGNSALEEAVS
                     LTRYASKVTIVHQFDHFQALEHYVDEAKKNEKINFIMESKIIEFIGDEKLEAVKIQNQ
                     STQENNEMKIDGVFIFIGYVPNTESLEGILELNQWKEIVVDKDMKTNLHGVFAAGDSI
                     QKRYRQVTTAVADGTIAALSASEYVNNLKKELSEHQLVH"
     gene            962..1576
                     /locus_tag="VF870_11885"
     CDS             962..1576
                     /locus_tag="VF870_11885"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9252937.1"
                     /translation="MTQNKDHWYDGWFYDTLIAPNQDRMFREIKNVIKPDSTIIDVGC
                     GTGRFSFSVADKVQKVVAIDLSKKNIDKANKTLSKNSNPKISFIHSNLSELVKINQHF
                     DYAVMTYVIHEVNPEERVALLKEMSQIADQIIIGDYLVPVKKGFWNILNEVVEFLAGK
                     EHYTNFKHFVANNGLARLTEKANLKIVTEIKNKPATSQILVLNK"
     gene            1660..>1925
                     /locus_tag="VF870_11890"
     CDS             1660..>1925
                     /locus_tag="VF870_11890"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9252938.1"
                     /translation="MNDRVYNNGVDRLRSPERVERLEIDRVVDLCLENNQIKSVLDVG
                     TGSGLFSEAFANRNMKIAGADLNPDMLEAAKKHLPDTEFKVAVA"
BASE COUNT          683 a          267 c          385 g          590 t
ORIGIN      
        1 tcattttgat gtaatagtta ttggcgcagg agctgctgga ctcacagccg ggatttatct
       61 ttcgcgagcc aaagttaaaa ctttagtttt aaatgaaggc acagtcggcg gacaaatggt
      121 tcttactcac aaaatcgcaa actatcccgg tgtagaaaat ataagcggat atgaacttgc
      181 aagaaccatg aaaacgcagg cagaaaaatt tggatgcacc atcaaatcaa atctaaaaat
      241 cactgctatg gatctcaaag acgaaatcaa aagagtagtt gtaaatacta aagagattta
      301 tacatctgac gctgttataa ttgcaaccgg gggaagatca agaatgattg gtgccattgg
      361 tgagggtaaa tttaaaggta aaggtatttc atattgtgca acctgcgatg gcgatttctt
      421 tcaggataag gaaattattg tggtcggcgg aggaaattct gctcttgaag aagctgtttc
      481 attaacccgg tatgcatcaa aggtaactat tgttcatcag tttgatcatt ttcaggcatt
      541 agagcattat gttgatgagg ctaagaaaaa tgaaaagatt aactttataa tggaatctaa
      601 aattattgag ttcattggtg atgaaaaact tgaagctgtt aaaatccaaa atcaatcgac
      661 acaagaaaat aatgaaatga aaattgatgg tgtttttatt ttcatcggtt acgttccaaa
      721 taccgaatca ttggaaggaa tattggaatt gaatcaatgg aaagaaattg ttgttgataa
      781 agatatgaag acaaatctgc atggagtttt tgcagcagga gattcaattc aaaaaagata
      841 tcgtcaggtt actacagcag ttgcagatgg gactattgca gcactaagtg catctgagta
      901 tgttaacaat ctaaaaaagg aattaagcga acaccagctt gttcattaat caaagtatgt
      961 aatgacacag aataaagatc attggtatga tggttggttt tacgatacac tgattgcacc
     1021 gaatcaggat aggatgtttc gagaaattaa aaatgttatc aaacctgatt caacaataat
     1081 tgatgttggc tgtggtacgg gcaggttttc attttctgtt gctgataaag ttcagaaggt
     1141 agttgcaata gatttatcaa aaaaaaatat tgataaagca aacaaaactc tttcaaaaaa
     1201 ttcaaatcct aaaatttcat ttatccactc aaatctttct gaattagtaa aaataaatca
     1261 gcattttgat tatgcagtta tgacttacgt tattcacgaa gttaatccgg aagaaagagt
     1321 cgcactttta aaggagatgt cccaaatagc agatcaaatt attatcggag attatcttgt
     1381 acccgtgaaa aaaggattct ggaatatttt aaatgaagtt gttgaatttc ttgccggtaa
     1441 agaacactac acaaatttta aacactttgt tgcgaataat ggtttggcca gacttactga
     1501 aaaagctaat ctcaaaattg tcactgaaat aaagaacaag ccggcaacga gtcaaatatt
     1561 agtattaaat aaatgataaa aattttaaat actgaatttt tctattttaa ttgtaattaa
     1621 tcgtttttgt tgtgtttaat ttattagagg aaatagttta tgaatgatag agtttataat
     1681 aatggggttg atcggcttcg ttcacctgag cgtgttgaac gattggaaat tgatcgagtt
     1741 gttgatttat gtttggagaa taatcaaatt aaatctgttt tagatgttgg aaccggaagc
     1801 ggattatttt ccgaagcttt tgcaaacaga aatatgaaaa ttgccggtgc tgatttgaat
     1861 cccgatatgc ttgaagcagc aaaaaaacat ctgcccgata ctgaatttaa agtagctgtt
     1921 gccga
//
LOCUS       DASWBY010000652         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_253438, whole genome shotgun sequence.
ACCESSION   DASWBY010000652 DASWBY010000000
VERSION     DASWBY010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253438"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..556
                     /locus_tag="VF870_11895"
     CDS             281..556
                     /locus_tag="VF870_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX9252939.1"
                     /translation="MAPDTGGKEEKYMKSKIKYTDEPMGKLKVVSDFLPPPDQLVLKE
                     DNVKVTLALKKSSVEFFKKEAKRHHTSYQKMIRQLVDWYSSHHQKSA"
BASE COUNT          561 a          314 c          359 g          537 t
ORIGIN      
        1 gtttaccata tatttatatt gcaatttaac ccccggctcg tggtaaacta atacaatgaa
       61 gagaatccac tttgaatggg atgaggtaga agataaggaa aatcagaaaa agcataatgt
      121 gtctttatcg cttgcacaac atgcttttct cgaccccacc gcataatcgt attggatttg
      181 gagcatagtg ctgaggagaa tcgatattat tgcatcggac gtgttggcga agggatcatg
      241 actgttcgat ttacgtttcg aggaaatgtt atccgtctgt atggcgccgg atactggagg
      301 aaaggaagaa aaatatatga agagcaaaat taaatatacc gatgagccaa tgggtaaatt
      361 gaaagttgtg agcgattttt tgccacctcc agatcagttg gtacttaaag aggacaatgt
      421 taaggtgaca ctcgccctga aaaaatcgag cgttgaattc tttaagaaag aggccaagag
      481 gcatcataca tcgtatcaaa aaatgattcg tcagcttgtc gattggtact cctctcacca
      541 tcaaaaaagt gcttaacgag ccattcgaga aggacggggt taagcactca ctcgagacat
      601 cattcagcac ataataggga gcgtacagta ttcttgggta aggtcatgtt tttaagctat
      661 ttgccaaacc tcgaatagaa gaagcacttg gagcatataa cccgaaggtc agaggttcaa
      721 atcctctccc cgctaccaaa tttcttaagg ggttacagct gcagctgtaa ccccgttttc
      781 ttttttaggc tgattttcta acggttttct aacggaatta aaagctccat acggaaaggt
      841 tattcggggt gtaacaatct tcgttgtagc agaggtgttg ataactgctc ttgtcactag
      901 actctttata atcagcaaag acaaacttct acagataaag gcatttgaaa cattttacta
      961 ttggtttaac gataaaaagg gatggttata ctcagaatta cgcaagttaa gagcatggca
     1021 gttggcaaga gaatggattg taaagctaaa gacaaagtta cgctttttga gaaggaaggg
     1081 acaatgagaa cccaaatttt gaaaagacca atataatctt gacgatttta ctgtttgaag
     1141 gttccgcttg tacatctcaa aatgcctatg acagtcttcg atatcatcag gtttctatcc
     1201 ttaattcggg attttaaatt attcatcatt cttttcatat gctcatcatt agatttatga
     1261 attaattgta tacaagcaac atgtcttatg ctatattgat tcgctgtgaa aatattattc
     1321 atcaattaaa attcaatatg gaggttcaga catgaagatt caggatattc agaaaattgc
     1381 aaagaagaaa ggcgttaata ttgggaaaat gaataaaaca gaacttatca gggctattca
     1441 aaaagcagaa gggaacaatg cttgcttttc tacttcttct gttcagacat gtggtcaaat
     1501 gaattgcctg tggcgtacag actgtaagta ggaatttaaa tttagtttcg tatagttgac
     1561 acttattccc accgtgagag catcaccttt cctctccgat tgcccctaat ttacctcgat
     1621 ttcaaatctt agacactaaa acaaaacttc ctttttcaat ttatccagtt tgctcataaa
     1681 tttttgtatt tccttccctc aaaaaggcat ttgggatatg atcggcacat tatgcgtgat
     1741 atctggtttg ctgatagcca tgttttggtg a
//
LOCUS       DASWBY010000653         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_742228, whole genome shotgun sequence.
ACCESSION   DASWBY010000653 DASWBY010000000
VERSION     DASWBY010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742228"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..>2134
                     /locus_tag="VF870_11900"
     CDS             45..>2134
                     /locus_tag="VF870_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX9252940.1"
                     /translation="MITIWKKVGIISTGIIVLMIPLSLLLHRPSNTLQKSEAQFVGGK
                     ECISCHQREYVLWKGSNHDNAMDIATDSTVLGNFDNVEVELFGKKHKFYKRDSKFFVY
                     TNGVGGKMSEFQITHTFGVRPLQQYLIPFDNGKYQCLPIAWDTNKKRWFDMAGLVYSQ
                     EDLKPDSWFYWTNQSQNWNGMCAECHSTNLQKNYNLESDSFNTTWSDIDVNCEACHGP
                     GSDHLDWAKLPEGARDYDGNLGLVLKTSGTTSKQFIEACAPCHSRRTSFGPNEHRDSE
                     YYNLNRPQNISPPLYYSDGQILDEDYEFGSFTQSKMYMNDVKCSDCHDSHSIKFKFEG
                     NALCTQCHRAEEFDTYQHHFHKYKNEKGTPVINKFGEISPVGDGALCVNCHMPGKYYM
                     GVDWRRDHSIRIPRPDISIKYNTPNACNNCHADKSFQWSEDYIKKYYGEKKKPTYGII
                     LSEGYLQKAGADTSLIKLIKSDLYPEIVRAAAISYLSAYNNAESNKTIKEMLNNTEPI
                     IRERSIEAFNSNDVKELVSIIAPLLNDPIRSVRMAAANKLSFVAKENFTDKQFKSLNA
                     AFEEYLSSLNYLADFPSGKYNLANYYSARQDYIKAEKFYNASIKGDSLFYPAKSNLAL
                     LYYNQGKLAEAEKIYLDLIKKHKEYTDGYYQLGLLYAEQQRYKEAALYLEKAALQVNA
                     NYRIYYNLGLLYQYL"
BASE COUNT          754 a          360 c          406 g          614 t
ORIGIN      
        1 ctcgatcgcg tttcgaaaaa tcacaaatta catacgttta atttatgatt actatctgga
       61 aaaaagtcgg aataatctca acaggcataa ttgtattgat gatccctctt tctctgttgc
      121 tgcatcgtcc atcaaatact ctacaaaaat cagaagctca atttgtgggt ggcaaagaat
      181 gtataagctg tcatcagcgg gagtatgtat tatggaaagg ttcaaatcat gataatgcaa
      241 tggatattgc aactgacagc actgtactag gaaattttga taatgttgaa gtggaattgt
      301 ttggtaagaa acataaattc tataaacgtg acagtaaatt ctttgtttac acaaatggtg
      361 ttggcggaaa gatgagtgaa tttcaaatta ctcatacctt tggtgtgcgt ccgcttcagc
      421 aatatttaat tccgtttgat aacggaaagt atcaatgcct gcctatagca tgggatacaa
      481 ataaaaaaag atggtttgat atggctggat tggtatacag tcaggaagat ttaaaaccgg
      541 acagctggtt ttactggact aaccaatcac aaaactggaa tggaatgtgc gcggaatgcc
      601 attcaactaa tcttcaaaaa aattataatc tggaatctga ttcattcaat acaacctggt
      661 ccgatattga tgttaattgc gaagcttgtc atggtccggg atctgatcat ctcgattggg
      721 caaaactgcc tgaaggagcc agagactatg atggaaattt aggtcttgta ttaaaaacaa
      781 gcggaacaac atcaaaacaa tttattgaag cctgcgcacc atgtcattcg cgaagaactt
      841 catttggacc gaatgaacac agagatagtg aatattataa ccttaatcgc ccgcagaata
      901 tctctccacc tctttactat tcggatggtc agatacttga tgaagattat gaatttggat
      961 cattcacaca aagcaaaatg tacatgaatg atgtaaaatg cagtgattgc cacgattcgc
     1021 acagcattaa atttaagttt gagggaaatg cgctttgcac acaatgtcat cgagcagaag
     1081 aattcgatac ttaccagcat cattttcata aatacaaaaa tgaaaaagga actcctgtaa
     1141 ttaataagtt tggtgaaata agtcccgttg gcgatggtgc actctgtgtt aattgccaca
     1201 tgcccggcaa atattatatg ggtgttgatt ggagacggga tcacagtata agaataccga
     1261 gacctgatat ttcaattaaa tataatacac cgaatgcctg caacaattgt cacgcagata
     1321 aaagctttca gtggtctgaa gattatataa aaaaatatta cggtgaaaag aaaaaaccca
     1381 cttacggaat tattctgtct gaaggatacc tgcaaaaagc tggagctgat acaagtttaa
     1441 taaaattgat aaagagcgat ctttatcccg aaatagtgag agcagcagca ataagttatt
     1501 tatccgcata taataatgct gaatctaaca agacgattaa ggaaatgctt aacaatacag
     1561 aaccgattat aagggaaaga tctattgaag catttaactc aaatgatgta aaagaattag
     1621 tttctataat agcaccatta ttaaatgatc cgattagaag tgttagaatg gcggccgcaa
     1681 ataagttatc atttgttgct aaagaaaact tcactgataa acaatttaag agtctgaacg
     1741 ctgcatttga agaatatctc agttcgttaa attacctggc tgacttcccc tccggtaaat
     1801 ataatcttgc aaattattat tctgccaggc aggattatat taaagcagaa aaattttata
     1861 atgcatctat aaaaggtgac agtctattct accctgcgaa atccaatctt gctttacttt
     1921 actataatca gggaaaactg gctgaagcag aaaaaattta tctggatctg ataaaaaaac
     1981 ataaagaata cacagatggt tattatcagc tagggctcct ttatgccgaa cagcaaaggt
     2041 ataaagaagc cgctttgtat ctagaaaaag cggctcttca agttaatgct aattacagaa
     2101 tctattataa cctgggcctg ttatatcaat atct
//
LOCUS       DASWBY010000654         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_139650, whole genome shotgun sequence.
ACCESSION   DASWBY010000654 DASWBY010000000
VERSION     DASWBY010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139650"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VF870_11905"
     CDS             complement(<1..163)
                     /locus_tag="VF870_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX9252941.1"
                     /translation="MMSQFPVVRHRRLRYNPLVRDMVRETVLTKNDLIYPLFVVPGEK
                     IKKEIKSMPG"
     gene            complement(206..589)
                     /locus_tag="VF870_11910"
     CDS             complement(206..589)
                     /locus_tag="VF870_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9252942.1"
                     /translation="MKVKEEAIKYGNVVLEKSFNFSVRILKLYRFLLKQDRSLEPILK
                     QVLRSGTSIGANITEAQNAPSTKDFINKLNIALKEAQESEYWLNLFKASDIIDEKSFK
                     SLQEDCKELIKLLTAIIKTAKSNSK"
     gene            complement(586..1974)
                     /gene="hemG"
                     /locus_tag="VF870_11915"
     CDS             complement(586..1974)
                     /gene="hemG"
                     /locus_tag="VF870_11915"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX9252943.1"
                     /translation="MNKKIVVIGAGISGLTSAYLLSKKGFDVTIIEKNNAVGGSIESV
                     TENGFLFDRGPNSALETTPVIGQLINELKLESELLYASKQANKRYILRDNILHSLPMS
                     PPGLIKTKLFSTKAKLRLMAEPFIGRSNDGYYQSLAEFVKRRLGQEFLDYAINPFVAG
                     VYAGRPEELSVKSAFPKLYALEEKYGGLIVGTVRSIRERKKRAEVAKQSAKMLSFKSG
                     MIALPKAIEKYFGNNILLSSEVTSIDKTEKGFAVSYQQNGATTKIECDYVLSTIPSYV
                     ASGLFAKFDKEFKTHADAIYYPPVLVYYLVYNTKDIKQDLDGFGFLIPAKENKSFLGA
                     LWSSVIFSDRTDETKAAFTLFVGGSRNPEFVKEERSALLTKVRKEFEALMGITSDPIF
                     TSERFWEKAIPQYNLGYVEHERFFDDFEKRNPGIFISGNFRGGISVGDCIKNADLVCD
                     KINLQFTMHNVQ"
     gene            complement(2144..>2378)
                     /locus_tag="VF870_11920"
     CDS             complement(2144..>2378)
                     /locus_tag="VF870_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9252944.1"
                     /translation="EFKDYADENLILVRLDFPKDIEQSAETKMYNSQLAQRYNVQGFP
                     TILLFNSQGRLVLQTGYQQGGPVNYVNHLKSYL"
BASE COUNT          716 a          443 c          381 g          838 t
ORIGIN      
        1 ctcccggcat cgatttaatt tctttcttaa ttttttctcc cggcacaaca aacaggggat
       61 aaattaaatc atttttagtt aacaccgttt ctctaaccat atcacgcaca agtggattgt
      121 aacgtaaacg acgatgacga acgacaggaa attgagacat cattttctcc tttttaatgt
      181 acaatgcaca atttacaatg tacaattatt ttgaattact ttttgctgtt ttaattattg
      241 ctgttaaaag tttgattaat tctttgcagt cttcttgtaa actcttaaat gatttttcat
      301 ctattatatc cgaagcttta aataaattta accagtattc agattcctgg gcttctttta
      361 atgcgatatt taatttatta ataaagtctt tggttgaagg agcgttttgt gcttcggtta
      421 tgtttgctcc aattgaagtt ccggatctta aaacctgttt taaaatgggt tccaatgaac
      481 ggtcttgttt taacaaaaat ctataaagtt ttaatatcct tacactgaaa ttaaaactct
      541 tttctaaaac aacgttgccg tattttatgg cttcttcttt taccttcatt gtacattgtg
      601 cattgtaaat tgtaaattaa tcttgtcgca gacaagatcg gcattcttta tacaatcccc
      661 aacagaaata ccgccacgga aattaccgct tataaatatg cccggatttc ttttttcaaa
      721 atcatcaaaa aatctttcgt gttcaacata tcccaaattg tattgtggaa tagctttttc
      781 ccagaatctt tctgatgtaa aaattggatc ggaagtaatg cccattaatg cttcaaattc
      841 ttttctaact ttcgttagca aagcacttct ctcctctttt acaaattcag gatttcttga
      901 cccgccaaca aataatgtaa aagccgcttt ggtttcatct gttctgtctg aaaagataac
      961 agaactccag agtgcaccaa gaaaagattt gttttctttg gcgggaatta aaaatccaaa
     1021 tccatctaaa tcttgtttta tatcttttgt attgtaaact aaataataaa ctaaaacagg
     1081 cggataataa attgcgtctg cgtgagtttt aaattcttta tcaaacttag caaacaagcc
     1141 gcttgcaaca taagatggta ttgttgataa cacgtaatca cattcaattt tggtcgttgc
     1201 accattttgt tggtatgaaa cagcaaaccc tttttcagtt ttatcaattg aagtaacttc
     1261 gcttgacaga aggatattgt tgccaaaata tttttctatt gcctttggca gagcaatcat
     1321 tccgctttta aacgataaca ttttcgcaga ttgcttcgca acttcggctc gcttttttct
     1381 ctcgcggatg cttctcacag tcccgacaat taatccaccg tatttttctt ctaacgcata
     1441 aagttttggg aatgcagatt taacacttaa ttcttcgggt ctacccgcat aaacaccggc
     1501 aacaaagggg ttgattgcat aatcaagaaa ctcctgccct aatcttcttt ttacgaactc
     1561 agctaaactt tgataatagc cgtcattcga tcttccaata aaaggctctg ccattaaacg
     1621 cagctttgct ttagtagaga aaagttttgt tttgattaac cccggtggag acattggcag
     1681 cgagtgaagt atgttatcgc gaagaatata tcttttgttt gcttgtttac ttgcgtagag
     1741 aagttcggat tcaagtttaa gctcatttat tagttgccca attacaggcg tggtttcgag
     1801 agcgctgttt ggtccgcggt caaacaaaaa tccattttcg gttacgcttt ctattgatcc
     1861 gccgacagca ttattttttt ctataattgt aacatcgaat ccttttttag atagaagata
     1921 agcggatgtt aaaccggata ttccagcacc tattactaca atttttttat tcattaggga
     1981 ttacgctttt tctttaactt gatcctgaac ttgaacttta ttgattacaa atttagcaaa
     2041 agataggcga agttttaatg cagaaagtgg gttataaata agcagaatag atattaaaaa
     2101 accttcgggg ccaaaagtgg aaaccccgaa ggttaaaatt aaattataag tagctcttta
     2161 aatgattaac ataattaacg ggtccacctt gctggtaacc agtttgtaaa accaatcgtc
     2221 cttgtgagtt gaacaacaaa atagttggaa atccctgtac attatatctt tgcgcaagct
     2281 gactgttata catttttgtt tctgcgcttt gctcaatgtc tttaggaaaa tctaaacgca
     2341 caagaattaa gttttcatct gcataatctt taaactca
//
LOCUS       DASWBY010000655         8873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_551611, whole genome shotgun sequence.
ACCESSION   DASWBY010000655 DASWBY010000000
VERSION     DASWBY010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551611"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..948)
                     /gene="xerD"
                     /locus_tag="VF870_11925"
     CDS             complement(58..948)
                     /gene="xerD"
                     /locus_tag="VF870_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX9252945.1"
                     /translation="MEMFLKEYLAHIKLEKNLSQNTVTSYKNDINAFLSYLENSGIDD
                     ASDISSNDVGGFFKTLRELGLSGSSSARYFSSLKGFFLYLLKNKYIIKNPIEKITAPK
                     ISKKLPGVLDINEVEKILAAPDVSEKLGMRDKAILELFYACGTRVSELINLKVNDLYF
                     DEEIIRVFGKGSKERLIPIGASAVTWVSEYLKKSRPLLMKKSKSENNLFLNSRGSKLS
                     RMGVWKIIDRYVKEAGIEKEVHPHTFRHSFATHLLEGGADLRAVQEMLGHADISTTQI
                     YTHIDRDYIKQIHKQYHPRG"
     gene            complement(948..3146)
                     /locus_tag="VF870_11930"
     CDS             complement(948..3146)
                     /locus_tag="VF870_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX9252946.1"
                     /translation="MNEQENTVKQTGIKRKIIIGLITVILIVLMFPKGESIESEVTEG
                     AIWTNDDLIAPFSFPIIKDIQTYQKEVRDAEKSVYPVFNSLDTKSNDSLKSFAEYITN
                     VIDENLDKRSEPYLNPTFLSSKAFTQFLNLRKQQRESYRGKDKALNNVFKYAENIAET
                     VNKHGILNFESDADIRDSIAVRVGNVDRIEPISKFYFYDQARRETRNKIRELNYSEEI
                     KDALIEFTVHFVYPNIVFNEEATNLEIEQAKNNVSKYSGIVNENERIIAKHDRITRQV
                     KLKIESYKEAKGDAIGSEGIILQLIGKFLHISFFITILAIYLFLFRKKIFYDNTKIVL
                     ISILVVFVSFVTYLINHIAIKAPIHFLIFVPAASMIFTIVFDSRVGFYLTVILSLIVG
                     ALRGNDYTFMAMNFIAGGLSVYTVRDIKNRTQIFRSFLFILIGYTASILAFGLERFAP
                     AETMLVEFAFAASNSLISPVLTYGLLIFFERLFNITTDLTLLELSNFDRPLLRELARK
                     APGTFNHSMTMGTIAEAAAEKIGANSLLARVGAYYHDIGKTIEPQGFVENQMNSQNLH
                     ENLPPKESAYMILKHVNEGIELAKQHKLPQELIDFIPMHHGTTVSTYFYEKAKTLYGE
                     DKVNINDYKYPGPKPNSKETAIVMLADGCESAVRSITEPDTNKVENMINNIFNLRLKE
                     GQLDDAPITFSDISKMKEAFLSILISQHHRRIKYPKQEDAEQGISTEKDK"
     gene            complement(3143..3997)
                     /locus_tag="VF870_11935"
     CDS             complement(3143..3997)
                     /locus_tag="VF870_11935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9252947.1"
                     /translation="MFDLNKLKKIEFVLFDVDGTLVDDSGEIGERTKQLIMDLKKLGV
                     LFSFASGRLHSALIPLANELKIYSPLISLDGSAIKSLTSKYFVYRSFLKQSQVKKAIE
                     YSEQYLLNIALCHTEAIYYTEQNSVIPKLIDKFGATYTEVNSYDDLTTETLEIVFAGD
                     NRRAVEFVRDKLSFPFTVGTSLSFFRSQTHDGIYYLEVRRSGSSKGKAMQRLLKYLKI
                     KETRTAVLGDWYNDISLFKSKGIKVAVSNAIPELKKSADYVLDKSNNEEGAAEFLEAL
                     LEAKNSQL"
     gene            complement(3999..5747)
                     /locus_tag="VF870_11940"
     CDS             complement(3999..5747)
                     /locus_tag="VF870_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX9252948.1"
                     /translation="MELIDYLIVIAYFIFSISIALIYSKRAGKSTNDFFLSGRNLPWY
                     LAGISMVATTFAADTPLAVTELVAKNGISGNWIWWNFAFGGMLTVFFFARLWRRAGIM
                     TEAEFAEIRYSGKPARFLRGFRALYLGLFMNVIIIGWVNKAMSSILQGMFGIDESLIM
                     FYVFGCMVLVAAYSAISGLWGVVITDAFQFFIAMFGCIVLAIIVVNSPEVGGIAGLKQ
                     NLPEYAFEFFPTISDIPSAAGMLSMSIFSFLAYIGIIWWASWYPGSEPGGGGYVAQRM
                     MSAKDEKHSLFATLFFQIAHYAIRPWPWILVGLSSIVLYPELADAQKGMGFIYAMRDF
                     LPAGLKGLLVAAFFAAYMSTIATQLNWGTSYIINDFYKRFINKGKEESHYVLSSRIAT
                     IVLMLISLVVTLLINKISGAWAFIIECGAGVGLVLILRWFWWRINAWSEISAMITPFI
                     IFPILKSVGILFPYSLFIIVPGTTLVWVIVTFLTKPTSEDVLFSFYRKIHPGGLLWKN
                     VSSKLPNVESDSGFFILFINWLIGVILVYSILFGTGKLILGEMTAFIIYLIIALISVS
                     IIYFNLSKIGWKTVIK"
     gene            complement(5961..7400)
                     /locus_tag="VF870_11945"
     CDS             complement(5961..7400)
                     /locus_tag="VF870_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX9252949.1"
                     /translation="MLVSTLDYVIIILYLIGITAFGKIIGGKQKTVKDYFVGKKEVSW
                     WIISFSIVAAETSTLTFISIPGLAYLTNLNFLQLTFGYLIGRIIVAQLFLPSYYKGDL
                     STAYSYLEKRFGNKTRSLASVTFLFTRTASDGVRLFATAIPLYLMLDISPMLAIIIIA
                     IVALLYTFTGGLKGVIWVDAIQMFIYIAGAILAGIFLINSIPGNISGIFSSPEISAKL
                     NIFNLGFNKGFTGFFSQPYTLLSGLIGGAFLSMSSHGTDQLIVQRLLAAKDLGESKKA
                     LITTGVIIIVQFALFLIVGVLLYAYYGSLNIKSDEIFSKFIIEVLPSGVKGIIIAGLF
                     AAALSTLAGSISSLSSSVMLDLYLPFKKSIDEEKNLLYSKLLTIFWAIMLIFSAFIFM
                     ESSKAVVELALSIASFTYGGLLGTFLLGLSNKKIQQNHAIAGFISAIIIMSFIIVFKV
                     VAWTWFILIGVCVTLLVGNLFELLTRKRN"
     gene            complement(7404..7748)
                     /locus_tag="VF870_11950"
     CDS             complement(7404..7748)
                     /locus_tag="VF870_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9252950.1"
                     /translation="MNFETKRVGDITVFKLNEKRLDTSISGLLKGEFTMILKIEGVNK
                     LIIDLSEVESCDSSGLSAILVANRIVSSNEGQIRLAAPSEKVHTLIRITQLDRVLPVT
                     DTVEHALAELGK"
     gene            complement(7758..8288)
                     /locus_tag="VF870_11955"
     CDS             complement(7758..8288)
                     /locus_tag="VF870_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252951.1"
                     /translation="MIDSIKENISRLLISRRLKNVVLNEQDFSESLKRSGSFLVLMPE
                     DEKDFRASYLVIEHLEHLNKSIKILTRDYRISLLPAKFRSKAMEFGIADLNKLNLPAH
                     KLIQKLSDMKFDGVIDLNRKDDLFYSYVANLVKAKIRIGFAKKGADNFYNFQISNNDI
                     ESEKSYNNFLNCLKMF"
     gene            complement(8281..>8873)
                     /locus_tag="VF870_11960"
     CDS             complement(8281..>8873)
                     /locus_tag="VF870_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9252952.1"
                     /translation="KREELIQIERLGEKNIDNLFRAIDVSKSQPFAKVLFALGIRYVG
                     AGAAQKITDHFTSIDDIIAASEEEISAIYEIGPSISKSIKQFFSDKKNIKLIEDLRKS
                     GLTFISEKKEIVQSAITGKIFVLTGTLSSLSRDEAGARIIALGGKVTSSVSKNTDFVV
                     AGEKAGSKLSKAESLGVKVLDEDTFLKMLEEKNNYD"
BASE COUNT         3004 a         1619 c         1299 g         2951 t
ORIGIN      
        1 taaataaaca caaaactgaa agcataacat atctcataaa taatttaatc atcatagcta
       61 acctcttgga tgatattgtt tatgaatttg tttaatgtaa tcacgatcaa tatgtgtata
      121 aatttgggtt gtagaaatat cagcatgtcc aagcatttcc tgtacagctc gtaagtctgc
      181 cccgccttca agtaaatgag ttgcgaagga atggcgaaaa gtatgcggat gaacttcttt
      241 ttctatgccg gcttctttta catatctatc tataattttc cacacaccca tcctggaaag
      301 ttttgatcca cgcgaattaa gaaataaatt attttcactt ttagattttt tcatcaacag
      361 tggtctgctt tttttaagat attcgctcac ccatgtaacc gcacttgcac caataggaat
      421 aagtctttcc tttgaccctt ttccgaaaac ccttataatt tcttcatcaa agtacagatc
      481 atttactttt aaattaatta attcagaaac tctcgtgccg catgcataaa atagttccag
      541 aatagcttta tcgcgcatac ctaacttttc actcacatct ggtgcggcta atattttttc
      601 aacttcattt atatctaaaa cacctggcaa tttttttgaa atttttggtg ctgttatttt
      661 ttcaatagga ttttttataa tgtatttatt ttttaagagg tacagaaaaa aaccttttaa
      721 tgaagaaaaa taccttgcac ttgatgaacc cgaaagacca agttctctta aagttttaaa
      781 gaatccgccg acatcatttg aagaaatatc tgatgcatcg tcgatacccg aattttcgag
      841 ataagatagg aaggcattaa tatcgttttt ataggatgta acagtgtttt gtgagagatt
      901 tttttccagt ttgatgtgtg caagatattc ttttagaaac atctccatta cttgtctttt
      961 tctgtgctga ttccctgttc agcgtcttct tgttttggat atttaattct tctatgatgc
     1021 tgactgataa gaatagaaag aaaagcttct ttcatttttg atatatcact gaatgtaatg
     1081 ggcgcatcat caagctgacc ttcttttaat cttaagttaa aaatattatt aatcatattt
     1141 tcaactttgt tagtatcagg ttcagttata gatctaaccg cagattcaca tccatctgca
     1201 agcataacaa tagcagtttc ttttgaattt ggttttggac cgggatattt ataatcgtta
     1261 atatttactt tgtcttcgcc atataaagtt ttagcctttt catagaaata tgtgctaact
     1321 gttgtaccat gatgcatagg aatgaaatct attaactcct gaggcaattt atgctgtttt
     1381 gctaattcta ttccctcatt aacatgttta agaatcatat atgcactttc tttcggaggc
     1441 aggttttcat gaagattttg actgttcatc tggttttcta caaatccctg aggttcaatt
     1501 gttttaccaa tatcatgata atatgcgccg actcttgcta gtaaagagtt tgcacctatt
     1561 ttttctgctg ctgcctctgc aattgtaccc atcgtcatcg aatgattaaa agtgcccggt
     1621 gcttttctgg caagttctcg taacaaaggc ctatcaaaat tagaaagttc taataaagtc
     1681 aaatctgtag ttatgttgaa aagtctttca aaaaatatta ataatccata agtcagaacc
     1741 ggactaatca atgaattgga agcagcaaaa gcaaattcta ctaacatagt ctctgccgga
     1801 gcaaatcttt ctaatccaaa tgcaagtatg gatgctgtgt aaccaattaa tatgaaaaga
     1861 aatgagcgga agatttgagt acgattttta atatccctta ctgtataaac agacaatccg
     1921 ccggcaataa aattcattgc cataaaagta taatcattgc ctcttagcgc accaacaatt
     1981 aaggaaagaa taactgttaa ataaaatccg actctggaat caaatacaat tgtaaatatc
     2041 atggatgcgg caggaacaaa aatcaaaaag tgtattggcg cctttattgc aatgtgattt
     2101 atcaggtaag ttacaaacga tacaaatacg accaatatag aaattaacac aattttagtg
     2161 ttatcataaa aaattttttt tctgaaaagg aataagtaga tggcaagtat agttatgaag
     2221 aaagatatat gtaagaattt accaatcagc tgtaatatta ttccttcaga accgattgcg
     2281 tccccttttg cttccttgta cgattctatt tttaatttaa cctgccgggt tatgcgatcg
     2341 tgctttgcaa taattctttc attttcatta actatgcccg aatatttaga aacattattt
     2401 tttgcctgtt ctatttctag gttggttgcc tcttcattaa atactatgtt agggtaaaca
     2461 aaatgaactg taaactcaat taaggcgtcc ttaatttctt cagaataatt aagttctctt
     2521 attttatttc ttgtttctct tcttgcctga tcataaaaat aaaatttact tattggttca
     2581 attctatcaa cgtttccaac tcttactgca atactatccc gaatgtcagc atcactttca
     2641 aaatttaata ttccatgttt attaacagtc tctgcaatat tttctgcgta cttaaaaaca
     2701 ttatttaatg ctttatcttt tccacgatat gactctcttt gctgcttgcg caaatttaaa
     2761 aattgtgtga aggcttttga acttaaaaat gtagggttta aatatggctc tgatctttta
     2821 tccagatttt catcaattac attggttata tattctgcaa aagattttag cgaatcatta
     2881 ctctttgtat ctaaactgtt aaatacggga tatacagatt tttcagcatc ccgaacttct
     2941 ttctgatatg tctgaatatc tttaattatt ggaaaactaa acggcgctat caaatcgtca
     3001 tttgtccaga tggctccttc ggttacctct gattcaatag actcaccttt cggaaacatc
     3061 aacacaatca ggataactgt aattaacccg ataattattt ttcttttaat tcctgtttgc
     3121 ttcacagtat tttcttgttc attcatagtt gagaattttt agcttctaat aaggcttcaa
     3181 gaaattcagc agcaccttct tcattattag atttatcaag tacataatct gctgattttt
     3241 ttaattcagg tattgcatta cttactgcaa cttttattcc tttagattta aataaactaa
     3301 tatcattata ccaatcacct aaaacagcag ttctggtttc ttttatcttc aaatatttaa
     3361 gtaaacgctg catcgctttt cctttagatg atccggatct tcttacttca agatagtaaa
     3421 ttccatcgtg agtttgggat ctaaaaaaag aaagcgaagt ccctaccgta aatggaaaac
     3481 ttaatttgtc tcttacaaac tctactgccc gtctgttatc acctgcaaaa actatttcta
     3541 atgtttcagt tgtaagatca tcgtatgaat taacctctgt ataagttgct ccaaatttat
     3601 caattagttt tggtatcacg gaattttgtt cagtataata aatcgcttct gtatgacaaa
     3661 gagcaatatt taaaaggtat tgttcagaat attcaattgc ttttttaacc tggctctgtt
     3721 taagaaaaga cctgtaaaca aaatatttac ttgtaagact ttttattgca gaaccatcca
     3781 aagaaattaa gggcgaatat attttaagct catttgctaa tggaatcaaa gctgaatgca
     3841 aacgaccgga tgcaaatgaa aaaagtactc cgagtttttt tagatccatt atcagctgtt
     3901 ttgttctttc cccaatttca cctgaatcat ctactaaagt accgtcaaca tcaaaaagta
     3961 caaattctat tttctttaat ttatttaaat caaacatttt attttataac agttttccag
     4021 ccaatttttg aaagattgaa ataaataatt gaaacggaaa ttaatgcaat aattaaataa
     4081 attataaaag ctgtcatttc acccaatatc agttttcctg taccaaataa aattgaatag
     4141 actaaaataa ctccaatcaa ccagttaata aacaatataa aaaatccgga atcactctca
     4201 acattaggta atttagacga aacatttttc caaagtaatc caccgggatg aatttttcta
     4261 tagaatgaaa aaagaacatc ttcgcttgtt ggcttggtta aaaatgtaac aattacccaa
     4321 accaaagttg tgcccggtac gattataaac aaactgtacg ggaaaagaat tccgacactt
     4381 tttaaaatcg gaaaaattat aaatggagta atcattgcag aaatttctga ccatgcattt
     4441 attctccacc agaaccatct taaaattaaa actaaaccga cacctgcacc acattcaata
     4501 ataaaagccc acgcaccgga tattttattt attaacaacg taacgaccaa agaaattaac
     4561 atcaaaacaa ttgtagcaat tcttgaagat aaaacataat gggattcttc cttccctttg
     4621 tttataaatc ttttgtaaaa atcatttatg atgtaggaag ttccccaatt taattgtgtt
     4681 gctattgtac tcatgtaggc tgcaaaaaaa gcggcgacta ataatccttt taaacctgca
     4741 ggaagaaaat cccgcattgc ataaataaaa cccatgcctt tttgtgcatc ggcaagttca
     4801 ggataaagta caattgatga caatccaact aaaatccacg gccacggtct tattgcataa
     4861 tgtgctattt gaaaaaacag tgtagcaaac aaagaatgtt tttcatcttt tgcagacatc
     4921 atacgctgag caacatatcc gccaccgccg ggctctgaac cgggatacca agacgcccac
     4981 catataatac caatgtacgc aagaaatgaa aaaatagaca ttgaaagcat tcctgccgcc
     5041 gaaggaatat cagatattgt tgggaaaaat tcaaaagcat actctggaag attctgcttt
     5101 aatcctgcaa taccgccaac ttccggtgag ttaactacaa tgattgcaag aacaatgcat
     5161 ccaaacattg caataaaaaa ctgaaatgca tctgtaataa caactcccca caaacctgat
     5221 attgcagaat atgcagcaac caaaaccata catccaaaaa cgtaaaacat aattagtgat
     5281 tcatcaatac caaacattcc ctgaagaatt gatgacattg ctttgttaac ccagcctatt
     5341 ataattacgt tcatgaataa accaaggtat aatgcgcgaa atcctcttaa aaaccttgcc
     5401 ggctttccgg agtatcttat ctcagcaaac tcagcttcag tcattattcc ggcacggcgc
     5461 cataatcttg caaagaaaaa aacagtaagc atgccgccaa aagcaaagtt ccaccaaatc
     5521 cagtttccgg aaataccgtt tttagcaaca agttctgtaa cagcaagtgg tgtatcagca
     5581 gcaaatgtgg ttgcaaccat tgatatgcca gctaaatacc aaggtagatt tcttcctgaa
     5641 agaaaaaaat cattggtgct tttacctgct cttttagaat aaattaaggc aattgaaatg
     5701 gaaaaaatga agtaagcaat aacgattaag taatctatta attccacatt tacctcttta
     5761 gtctcggttt atctgctgtg tgggaaatgt tttttttcaa tgataaatat aaggtttagt
     5821 tatgaaaatg gatacttaaa cttttacatt tattattaag tatggctaaa aagtttagga
     5881 attttgggca ggtattaaaa tttatataat actgaatgct ttagaataaa ttgttttgag
     5941 taagaaactc aaattgtaaa tcaattacgt tttcttgtaa gcaactcaaa taaattacca
     6001 acaagaagtg taacgcaaac accaattaaa ataaaccacg tccaggcaac aaccttaaat
     6061 actattataa aactcataat aataattgcg gagataaatc ctgctatggc atgattctgc
     6121 tgaatctttt tgtttgataa tcccaataaa aaggttccta acaaaccgcc gtaagtaaag
     6181 gaagcaatgc ttaatgcaag ctcaacaact gctttagaac tttccataaa aataaaagca
     6241 gaaaaaatca gcattattgc ccaaaagatt gttaatagct ttgagtacaa taaattcttt
     6301 tcttcatcaa ttgatttttt gaatggaaga taaagatcta acattactga ggaagaaaga
     6361 gaagatattg atccggcaag agtagaaagt gccgcggcaa ataatccggc aataataatt
     6421 cctttaactc cggatggtaa aacttcaatg ataaatttag agaatatttc atcagattta
     6481 atatttagtg aaccataata tgcatagagc aaaaccccta ctatcaaaaa caaagcaaac
     6541 tgaactatga ttataacgcc cgttgtaata agtgctttct tgctttcacc aagatctttc
     6601 gcagctaaca gtctttgcac aataagctga tcagttccgt gtgatgacat tgagagaaaa
     6661 gcaccgccaa tcaatccgct taataatgta tatggctgag aaaagaatcc ggtaaaacct
     6721 ttattaaaac ctaaattaaa tatgttaagc ttagcagata tttccggaga ggaaaaaatc
     6781 ccgctaatat tcccaggaat actatttatc aaaaatattc cggctaaaat tgctccagct
     6841 atataaataa acatctggat agcatctacc cagattactc cttttaatcc ccccgtaaaa
     6901 gtgtaaagca gcgcaacgat tgcaatgata ataattgcaa gcatcggaga aatatcgagc
     6961 attaaataaa gcggaatagc tgttgcaaat agtcttacac catctgaagc ggttctggta
     7021 aataaaaaag ttacagaggc caatgaacgt gttttattac caaatctttt ttcaagatat
     7081 gaataagcgg ttgacagatc acccttataa taggatggta aaaacaactg cgcaacaatt
     7141 attcttccaa ttaaatatcc gaatgttaat tgaagaaaat ttaaattagt cagataagca
     7201 agaccgggaa ttgagataaa agttaaagta cttgtttcag cggcaacaat tgaaaaactg
     7261 attatccacc atgaaacttc ttttttacca acaaaataat ctttaacagt tttttgttta
     7321 cccccgataa tttttccgaa ggcagtaata ccaatcaggt ataaaataat tattacgtaa
     7381 tcaagtgtgg aaacaagcaa atattatttt cccaactctg ctaatgcatg ctcaacggta
     7441 tcagtaacag gaagtactct atctaattga gtaattctta taagggtatg cactttttca
     7501 gatggggcag caagtctaat ctgcccttcg tttgatgaga caattctgtt agccacaaga
     7561 attgcactta aaccagagct atcgcagctt tcaacttcac tgagatcaat tattagttta
     7621 tttacgcctt ctatttttag aatcattgta aattcacctt taagcaatcc ggatatgctc
     7681 gtatccagtc gtttttcatt caatttaaaa acagtaatat caccaacacg ctttgtttcg
     7741 aagttcatag tattacctta aaacattttt aaacaattta agaaattatt ataagatttt
     7801 tctgattcaa tatcgttgtt cgaaatctga aaattataaa aattatcagc accttttttt
     7861 gcaaacccaa tcctaatttt ggctttaact aggttcgcaa catagctata aaaaagatca
     7921 tccttacggt tcagatctat caccccgtca aatttcatat ctgataattt ctgtatcagt
     7981 ttgtgtgcag gaagattcag tttatttaaa tcagcaattc caaattccat tgctttgctt
     8041 ctaaactttg ccggaagcag actgatacga tagtcgcgtg ttaagatctt tatagattta
     8101 ttcaggtgct caagatgttc aatcacaaga taacttgctc taaaatcttt ttcatcttca
     8161 ggcatcagta ctaaaaacga accggatctc tttaaagact cagaaaaatc ctgttcattc
     8221 aaaacaacat tttttaatct tcttgaaatt aataatcgcg agatattttc tttaattgaa
     8281 tcaatcataa ttattttttt cttcaagcat ttttaggaat gtatcttcat ccaatacttt
     8341 tacacctagt gactctgctt tagataattt agaccctgcc ttttctccgg caacaacaaa
     8401 atcagtattc ttgctaacac ttgatgttac tttaccgcct aatgcaatta ttcttgcacc
     8461 agcttcatca cgggaaagcg aagaaagagt tcctgttagt acaaatattt ttcctgttat
     8521 ggcggattgt actatttctt ttttttctga tataaaagtt aaacctgatt tacgtaaatc
     8581 ttcaatcaac ttaatatttt ttttatccga aaagaactgt ttaatacttt tacttatgct
     8641 tggaccaatt tcataaatcg cagaaatttc ttcttcgctt gctgcaataa tatcatcaat
     8701 tgatgtaaaa tgatctgtaa ttttttgtgc cgcacctgct cccacatacc ttattcccag
     8761 tgcgaataaa acttttgcaa aaggttgtga tttactaaca tcaatagcac gaaaaagatt
     8821 atcaatgttt ttttcaccta aacgttctat ctgaattaat tcttcgcgtt tat
//
LOCUS       DASWBY010000656         5498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_139744, whole genome shotgun sequence.
ACCESSION   DASWBY010000656 DASWBY010000000
VERSION     DASWBY010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139744"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VF870_11965"
     CDS             <1..745
                     /locus_tag="VF870_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX9252953.1"
                     /translation="TAEQFYSFRNNVQNIDTIFSNHYPEKDFNGKLALASIELFNSTN
                     IPKYLEDAILYGKKADSDFWWSVGDINSLAHYKIALHNPDFAKYIYQNLEHSKKTSYK
                     SFLHEGLEYSWGTTNAFLGVSLQTILYKKLTGSTEFDSLNIYQRDYILGRNPWGKSFI
                     YNIGTDFPKNLHSQVAFFNKGYLPGALTAGPAPSDLLANYKIQRINNSDEFNSNYTKY
                     YDDRMDFITNEPTIVGNATALFVFGFLSN"
     gene            936..2141
                     /locus_tag="VF870_11970"
     CDS             936..2141
                     /locus_tag="VF870_11970"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX9252954.1"
                     /translation="MKKSVQLFSSGIPIVDNAWGGFYHGGTYLLVGEKKSGKTLLSLQ
                     YAVEAAKNKQVCLYFTNSRPKDLMIHAASIDIDLENYINQNLIIVVRVAQPTETTEFK
                     SRDEFLSDYLHDIISVINQYNPTRIIFDELTPYVEYENLNSLRESYGSMIESIEDLGI
                     TSLFILREPAAQSSKQIFNVLNSFATGIIQLHKSEDSEEQQPGIIDITPNIGHTEGKF
                     KANYFIEPYKGITTDFKISKRSTRENILPEITELVPGKTSGIISQEYLPPNIYSISDF
                     KLIINNQIALYKTTGQMFTILSLRLNPLSQLQSRISFEQLDYAVKLAANKKDKVCTID
                     DKIYILITKSDDNLIDGFISKVHQSLSVSGSANVSYLMLKVNNTMNSADDIFKEFESE
                     TARTRLNQR"
     gene            2157..5003
                     /locus_tag="VF870_11975"
     CDS             2157..5003
                     /locus_tag="VF870_11975"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9252955.1"
                     /translation="MKLFFYQLLILNLTFLSISLFSQNFNSANYKSEALQLMSSGRYG
                     EAIDVLNKYVSANPNSADGFNLRGICYEKRGNYEYAVYDFRTAKKIKPNDDEISSNLN
                     RTTSDWYKLLYNKIEGHKREIAINPSIAKNYLEIGKSYKNLGNWNEAEIWYDIFLEKE
                     NASPDEIIRYSEILAKNNHISKGETILKSYIERFPDDHRLWSRYGYFTLWLGKNKIAI
                     QAFTKSLEIRPYFKEAMDGLDLAKGKGYVYSINDTTSRYNYGMPLPVKQYEIDRLYRV
                     LKNNPGDDESRFKLIDELMASNRYEEAFQQLQILSPNYSDQDRFKELWFKITTLRKSY
                     YADRIRFYEEQLSKDPANKKALLELAKFYSYNNDYTLAVNLYKSYLLKYPIDNEARYK
                     LAQILMWQNNLCEATEIAEELIKSSPGNKDYLLLAAQINYWLDKDLVYTKSLFERVLV
                     LDPKNSEAMYGLANLYIKIDNLSDAEKLIGKISLIDSSSQQFILLQKSFEIAQTQNEL
                     VQSNKILEEARMYSSNKDFDSSIKSFKNYLANYPNNKQVKLELANVYIANSELDKSIK
                     IYDELLKSSSDYEIEKQRAKVYLWSGDSLSALREFRRLNQKYPDDIETKLLLGDSYLQ
                     AGQEQNARIIYEDLLLKSPDSHILKTRLGWLGGSDKFSLNKFPTYIQLIPRASYFTDN
                     TDFSYSNIGLGFDFGATNYLAFGFSASGGQISSAEDNLRFNQIKGSAYVRFNEIFSGV
                     GSFGQTYFVNDLQENVIEISLTAQKKNIFNVIAFLNYSDAAFILYSPFLVNTRLNAYY
                     FGLNGEYKFKNNLVISGKYSYVDVSDENSGNQFVARLGKVFESELTAGYEYYFYSFKT
                     ETQLYWSPKNFESHSIWADWILYKDDTFDFTIGGKIGLIPQNDYILSEFYASINYQIV
                     KNLLFQTRFTTGSSFRSNTGYRSNSIQASLIWGL"
     gene            5187..>5498
                     /locus_tag="VF870_11980"
     CDS             5187..>5498
                     /locus_tag="VF870_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252956.1"
                     /translation="MRINQLVDRAIITISSREDEKRGENLEQKYLRRILVSGIVSLLL
                     ILVIVYTLPYTILSYSSLFEYSTIIALVIFLFILLVRYFAILIMAYLYINEYTFSDTN
                     GY"
BASE COUNT         1976 a          837 c          883 g         1802 t
ORIGIN      
        1 tactgcagaa caattttatt catttagaaa taatgttcag aatatcgata caattttttc
       61 aaatcattat cctgaaaaag attttaatgg aaaattagcg cttgcttcta tagaattatt
      121 taacagcaca aatatcccga aatatcttga agatgcaatt ttatatggca aaaaagctga
      181 ttcagatttt tggtggagtg ttggggatat taattctttg gcacattaca aaattgctct
      241 tcataatccg gattttgcaa aatacattta tcaaaatctt gaacattcaa aaaaaaccag
      301 ctataaatct ttcttgcatg aagggttaga atattcctgg ggtacaacaa acgcatttct
      361 tggagttagc ttacaaacaa ttctttataa aaaattaacc ggttcaactg aattcgattc
      421 actaaatatt tatcaaaggg attatatttt aggaagaaat ccctggggta aaagttttat
      481 ttataatatc ggtacagatt tcccaaaaaa tctgcactcg caagtagctt tttttaacaa
      541 aggttatcta cccggggcac ttactgctgg accagcacca tctgatcttt tagcaaatta
      601 taaaatccaa agaataaaca acagtgatga gtttaactca aattacacaa aatattatga
      661 tgatagaatg gattttataa caaacgaacc aacaattgtt ggaaatgcca ccgcgctttt
      721 tgttttcgga tttttgagta attaataaca ttattcatac aaattagtac cataaacctt
      781 tgaatttatc aaatattaag cttgttgcat aataatacaa cttaatgttt ttgtctctgt
      841 ttagtccaaa aaaatatctt ttttattcat tttttggaca taatcaagct aacctaacgg
      901 tacaattatt ggtaaaattg caaaaaaaat aatctatgaa aaaaagcgta caattattta
      961 gttcagggat tcctatcgtt gataatgcct ggggaggatt ttatcacggc ggaacttatt
     1021 tgttagtcgg tgaaaaaaag tctggcaaaa ctttgcttag ccttcaatat gccgttgaag
     1081 ctgctaagaa taaacaagtt tgcctttatt tcacaaactc ccgccctaag gatttgatga
     1141 ttcacgcagc atcaatagat atcgatctcg aaaattacat taatcagaat ttaattatcg
     1201 ttgtacgtgt tgcacagccg acagaaacaa ccgaatttaa aagcagggat gaatttttaa
     1261 gtgattatct gcacgatatt atctcagtaa ttaatcaata taatccaact agaattattt
     1321 ttgatgaatt aactccatat gttgagtatg aaaatcttaa tagtctcaga gaatcatatg
     1381 gctcgatgat agagtctatt gaagatttgg gtattacaag tttatttatt ttaagagaac
     1441 cagcagctca atcttctaag cagatattta atgttctgaa ttcttttgca accggaatta
     1501 ttcaacttca taaatcagaa gacagtgaag aacagcagcc tggcataata gatattactc
     1561 cgaatatcgg acataccgaa ggaaaattta aagcaaacta cttcatagaa ccgtacaaag
     1621 gtataaccac tgattttaaa ataagtaaac gttcaaccag ggaaaatatt ttacctgaaa
     1681 ttactgaact agtacccggc aaaacgagtg gtattatctc tcaggaatat ttaccgccta
     1741 acatatacag cattagtgat tttaagctaa tcataaataa tcagattgca ttgtataaaa
     1801 caaccggaca aatgtttact attttatctc tcagactcaa tccgctaagt caattgcaat
     1861 cccgaatatc atttgaacaa ttagattatg cagtaaaatt agccgctaac aaaaaagata
     1921 aagtctgcac aattgatgat aaaatatata ttcttataac taaatctgat gataatttaa
     1981 ttgatggatt catttcaaag gttcaccaat cattaagtgt cagtggatca gcaaacgtat
     2041 cttatttaat gttaaaggta aataacacaa tgaacagtgc tgatgatatc tttaaagaat
     2101 ttgaaagtga aactgccaga acccgattaa atcagagata gaattgacta tagaagatga
     2161 aattattttt ctatcaatta cttattctga acctgacttt tcttagcatc tctttattct
     2221 ctcaaaattt taactccgct aattataaat ctgaagctct tcaactgatg agttccgggc
     2281 gttatggtga ggctatagac gttctaaata aatacgtttc cgcaaatcca aactctgccg
     2341 atggatttaa tttacgaggc atatgttatg aaaagagggg aaactatgaa tacgctgttt
     2401 acgactttcg cactgcgaaa aaaataaaac caaacgatga tgaaatttca tcaaatctaa
     2461 acaggacaac ttcggattgg tataaactcc tttataataa aattgaaggg cataaaagag
     2521 aaattgcaat aaatcccagc attgctaaaa attatcttga gatagggaaa tcctataaaa
     2581 atttaggcaa ctggaatgaa gcagaaattt ggtatgatat atttttagaa aaagaaaatg
     2641 cttctcctga tgaaatcata aggtactctg aaatattagc caagaataac catatctcta
     2701 aaggtgaaac gattttaaaa tcatatatag agcgcttccc tgatgatcat agattatggt
     2761 caagatacgg ttactttaca ttatggcttg ggaaaaataa aattgctatc caggcattta
     2821 ctaaatcgtt agagattcga ccttacttta aggaagcgat ggatggattg gatttagcaa
     2881 aaggcaaagg ttatgtttat tctattaacg ataccacttc acgttataat tatggaatgc
     2941 cgctaccggt taaacaatac gaaattgata gattatatcg cgtcctcaaa aataatccag
     3001 gtgatgatga aagcaggttt aagttaattg acgagttaat ggcttcaaat cgttatgaag
     3061 aagcttttca acaattgcag attttatcac ctaactattc agatcaagat agatttaaag
     3121 aattatggtt caagataaca actttaagaa aatcttatta tgctgacagg ataagatttt
     3181 atgaagaaca attaagtaaa gatcctgcga ataaaaaagc cctgttagaa ttggctaaat
     3241 tttattctta taataatgat tataccctgg cagtaaatct ttataaaagt tacttgttaa
     3301 aatatccgat tgataatgag gctagataca aattagccca gattctgatg tggcaaaaca
     3361 atctctgtga agctacagag atagctgaag aacttattaa atcttctccg gggaataaag
     3421 actatttgct gcttgctgcc caaattaatt attggttaga taaagatttg gtatatacca
     3481 aatcactttt tgaaagagtt ctggttttag atcctaagaa ttccgaggct atgtatggtt
     3541 tggctaatct gtatataaaa attgataacc tctctgatgc agaaaaatta atcggtaaaa
     3601 tttcattaat tgatagctca tctcagcaat ttattttact acaaaaaagt tttgaaattg
     3661 ctcaaaccca gaatgaactt gttcaatcaa ataagatatt agaagaagcg cgaatgtatt
     3721 cttctaataa agattttgat tcatcaataa aatcatttaa aaattatcta gcaaattacc
     3781 ctaataataa gcaggtgaaa ctcgagttgg caaatgttta tattgcaaat tcagaattag
     3841 acaaatcaat aaaaatttat gatgagttgt taaaatcgtc cagtgattat gaaatagaaa
     3901 aacaacgtgc aaaggtatat ttatggagtg gggattcact ttcagcatta agagaattta
     3961 gaagattgaa tcaaaaatat ccggatgata ttgaaactaa attgctgcta ggtgattctt
     4021 atctacaagc aggacaagaa cagaatgcaa gaattattta tgaagatctt cttctgaaat
     4081 ctccagactc acacatacta aaaacacgtt taggctggct tggcggaagt gataagtttt
     4141 cattaaataa gtttcccact tatatccaat taattccaag agcatcttac tttacagata
     4201 ataccgactt tagctattct aacattggtt taggatttga ttttggggcg acaaactatc
     4261 ttgcatttgg tttttctgct tcaggcgggc aaatttcttc cgctgaggat aatttaaggt
     4321 ttaatcaaat taaaggatca gcgtatgtaa gatttaacga aatattttcc ggtgttggaa
     4381 gttttggaca aacatatttt gttaatgatc ttcaggaaaa tgtaatagaa atctcactta
     4441 ctgcacagaa gaaaaatatt tttaatgtaa ttgctttctt aaattattct gatgccgcat
     4501 ttatacttta ctcaccattc ttagttaata caagattgaa tgcatattat tttggtctca
     4561 atggtgaata taagtttaaa aataatttag taatctccgg aaaatattct tatgtggatg
     4621 tctcagatga aaattcaggt aatcaatttg tagcaaggtt aggaaaagtt tttgagtccg
     4681 agctaactgc cggatatgag tactattttt attcgtttaa aactgaaact cagctttact
     4741 ggagtccaaa gaattttgaa tcacattcta tctgggcaga ttggatactt tataaagatg
     4801 atacttttga ttttactatt ggcggcaaaa ttggattaat tccacaaaat gattatatac
     4861 tttccgaatt ttatgcatcc attaattatc aaatagttaa aaacctgctt ttccaaacta
     4921 gatttacgac cggaagttcg tttagatcaa atacaggtta tcgatcaaat tctatccagg
     4981 ctagccttat ttggggattg taaccagcta tttttaagat tttatctcca aaacttaatt
     5041 atcacccttg tttgataatg atacagatta aattaaagtc caatattggt aatgatattt
     5101 cgtcaaatta ttcgaattaa aagttaaata aactgaagat aatggcatta ttattgaaaa
     5161 attgaagcaa accatcaacc gggaaaatga gaataaatca gcttgtcgat cgtgctataa
     5221 ttactatttc aagccgggaa gatgaaaaaa gaggagaaaa tctagagcaa aaatatttac
     5281 gaagaatact agtaagcggt attgtttctc ttttactgat cctagtaata gtttatactt
     5341 taccttacac aattttgtca tacagttcac tttttgagta ttcaacaatc attgctctgg
     5401 ttatttttct attcatcctt ctagtaagat attttgccat tttgatcatg gcgtatttat
     5461 atattaatga atacactttc tccgatacaa atggttat
//
LOCUS       DASWBY010000657         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_289858, whole genome shotgun sequence.
ACCESSION   DASWBY010000657 DASWBY010000000
VERSION     DASWBY010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289858"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..384
                     /locus_tag="VF870_11985"
     tRNA            299..384
                     /locus_tag="VF870_11985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Leu,seq:caa)
     gene            465..1040
                     /locus_tag="VF870_11990"
     CDS             465..1040
                     /locus_tag="VF870_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9252957.1"
                     /translation="MGLSLSKIIIITFISVLLSLIYNHFNPIGLRLIREDKKLVWESD
                     SQLSSDKVDSSLSYKGPIETKSPESVPSGKNELKSEPFKEPKAIKLDYAFKLFKDGIQ
                     FIDARSPEEFAEGHIKGAINIPFYGSENYLDAINHLNKNEFIVTYCSGADCDISILSG
                     NELFEMGFKRVYVFIGGYDEWTKNHYPITKN"
     gene            1045..1482
                     /locus_tag="VF870_11995"
     CDS             1045..1482
                     /locus_tag="VF870_11995"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX9252958.1"
                     /translation="MNKIFSNRYLLFIIRIILGFVFIYAGAEKISDPEAFAISISNYR
                     LLPIAIINFFAITLPWIELVAGILIVFGISVKENSAIIFTMLLVFTVAIIISLFRGLS
                     IDCGCFGKGTQIGLLKLGENTLMIIGSFLLMVFGSNIFRLKSD"
     gene            complement(1485..>2713)
                     /locus_tag="VF870_12000"
     CDS             complement(1485..>2713)
                     /locus_tag="VF870_12000"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252959.1"
                     /translation="KKNSVFAEIIPTKSNRNSALKTYQFAKENKIPIVASNPIFFLEK
                     KDYELHKLVTAIRLNKAITSISNDELVDEEFYFKDKSELENKFSKLPEAIANSDKIAN
                     ECKVDLNLGQYKYPSYQTDYKENTSTLLNSIAFQGLKKRYGYIDNKMKTRLKYELSVI
                     NELNFTDYFLIVWDIVQEAKRRGMMTIGRGSAANSLVAYCIELTQVDPIKLNLYFERF
                     LNKARSSPPDVDIDFSWKERDEIVKYVFEKYGYEKVAMISTTITFRARSAFRETAKAF
                     GISEREISKYSKKIPWTNAVNLPKISEMFPESKNLEFDKEPWKTIVKLASRIANFPRH
                     LSIHPSGIVITQTKITDYCALEYAKNKGLGLIITQPDMYSIEELGLVKIDLLSQRSLG
                     VLRDTMETINKSDMAE"
BASE COUNT          861 a          425 c          415 g         1012 t
ORIGIN      
        1 atataattct tcatagacta aatatctttt tatcaatgta tattaatcag ggacgagaat
       61 agcaaaacta tattaatatg agaaatagga ttattaacaa gaaattatat atcataagga
      121 atttatgaga acacttaatt gcattttttc aagcttaaaa tcaaattgct tcctaaccaa
      181 ttcttgatta attttcgtta gaacttaaat caaataaatt acagcaaatt tgcaacgtaa
      241 ctttgttatc aattggattt gagggtaata aagtttcatt ttttgattaa aaactaatgc
      301 cgaaatggcg gaattggtag acgcgctgga ctcaaaatcc agtggggctt acacctcgtg
      361 ccggttcgag tccggctttc ggtactctcg cctcctttta ggaggctttt tattaaatgt
      421 tggtttagaa tatttaaata acaaatcata aaaatattac attgatgggc ctaagtcttt
      481 caaaaataat tattatcact tttatttcag ttctattatc tctcatatat aaccatttca
      541 atcctattgg attgagactt attagagaag ataaaaaatt agtatgggaa tctgattctc
      601 aactttcatc agataaagtt gattcttctc tttcatataa aggtccgatc gaaacaaaat
      661 ctccagagtc tgttccatcc ggaaaaaatg aattgaagtc agaaccattt aaagaaccca
      721 aggctatcaa attagattat gcttttaagt tatttaagga tggaattcag tttattgatg
      781 cccgttcacc tgaagaattt gctgaaggac atataaaagg tgccataaat attccttttt
      841 atggatctga aaactattta gatgctatta accatttaaa taaaaatgaa tttattgtta
      901 cttattgcag cggggcagat tgtgatataa gtattctatc aggcaatgaa ctttttgaaa
      961 tgggatttaa aagagtgtat gtttttattg gtggctacga tgaatggaca aagaatcatt
     1021 atccaattac taaaaattag ttagatgaat aaaatatttt caaatagata tcttcttttt
     1081 attattagaa taattctcgg ttttgttttt atttacgccg gtgctgaaaa gatatcagat
     1141 cctgaagcat ttgcaatttc catttcaaat tatcgcctgc ttccaatagc aatcataaac
     1201 ttttttgcaa ttactttacc ttggatagaa ttagttgcgg gtattttaat tgtctttgga
     1261 atttcagtca aagaaaattc tgctatcatc ttcactatgc ttttggtttt tacagttgca
     1321 ataatcatta gtttatttcg tggattaagt atcgattgcg gttgttttgg gaaaggtact
     1381 cagattggat tattaaaact tggagaaaat actttaatga ttataggttc ctttttacta
     1441 atggtgtttg gatccaatat tttcagactt aaatcagatt agaattattc cgccatatca
     1501 cttttattaa tggtttccat cgtatctctt aatacaccaa gtgatctttg acttaaaaga
     1561 tcaatcttaa ctaaacctaa ttcttcaata gagtacatat cgggctgagt tattattagt
     1621 cccaaacctt tattcttcgc atactcaaga gcacagtaat cagttatttt agtttgagtg
     1681 attacaatcc cgcttggatg aatacttaga tgtcttggga agtttgcaat tctggaagca
     1741 agttttacga ttgttttcca gggttcttta tcaaattcca aatttttaga ttcaggaaac
     1801 atctcggaaa ttttaggcag atttaccgcg ttagtccatg gaattttttt gctgtactta
     1861 gatatttctc tttcagaaat tccaaatgcc tttgctgttt ctctaaatgc tgaacgcgca
     1921 cgaaaagtaa ttgtcgtaga aatcatagca actttttcat aaccatattt ttcaaaaaca
     1981 tacttaacaa tttcatcccg ctctttccag gagaaatcaa tatcgacgtc aggtggactg
     2041 cttcgcgctt tatttaaaaa tctctcgaag taaaggttta gttttatagg atctacctga
     2101 gtcaattcaa tacaataggc taccaggcta tttgctgcag atcctcttcc aattgtcatc
     2161 attccccttc ttttagcttc ctgaacaata tcccaaacaa ttaaaaaata atcggtaaag
     2221 tttagctcgt ttataacact taactcatac tttaatcgtg ttttcatttt attatcgata
     2281 tagccatatc ttttttttag accttgaaat gcaatactat tcaataatgt agatgtattt
     2341 tctttgtaat ctgtttgata agatgggtat ttatattgac ctaaatttaa atcgactttg
     2401 cattcattcg ctattttatc agaattagca attgcctcag gcaattttga aaatttattt
     2461 tctaattctg atttatcttt aaaataaaac tcttcatcaa ccaattcatc attggatatt
     2521 gaagtaatag ctttattaag tcttattgca gttacaagtt tatgcagttc atagtctttc
     2581 ttttctaaaa agaaaattgg atttgacgca acgattggaa ttttattctc ctttgcaaac
     2641 tgatatgtct taagcgcact atttctgttg gatttggttg gtatgatctc agcaaaaaca
     2701 gagttttttt tat
//
LOCUS       DASWBY010000658         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_101759, whole genome shotgun sequence.
ACCESSION   DASWBY010000658 DASWBY010000000
VERSION     DASWBY010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101759"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VF870_12005"
     CDS             <1..231
                     /locus_tag="VF870_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX9252960.1"
                     /translation="FDNWIFGCDICQDVCPWNQKFSQQTWIPEFYPYNKEFNYNEIMK
                     LDEQSFKQRFAESPIKRTKLKGLQRNAKFLFE"
     gene            323..1285
                     /gene="trxB"
                     /locus_tag="VF870_12010"
     CDS             323..1285
                     /gene="trxB"
                     /locus_tag="VF870_12010"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX9252961.1"
                     /translation="MSEQANQKNHFKVLIIGSGPAGLTAAIYTGRANLSPVIFEGMQP
                     GGQLTITTEVENYPGFEHGIDGPALMDVMRKQAQRFGAQSFYKEVTEVDFSSRPFKVK
                     SYDENYTADSIIISTGASAKLLGLESEKKYMGYGVSACATCDGFFFKGLKIIVVGGGD
                     TAMEEANFLTKFADEVTIIHRRDEFRASKIMTERAQKNPKIKFMTNKIVKEVIGVEEN
                     GKKSMTGVILEDTKDHSLTEFKADGMFLGIGHQPNTKLFKGFLDMDETGYLITKPGST
                     YTNIEGVFAAGDVADHVYRQAVTAAGTGCMAALDAERWLETQEA"
     gene            complement(1324..1674)
                     /locus_tag="VF870_12015"
     CDS             complement(1324..1674)
                     /locus_tag="VF870_12015"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEX9252962.1"
                     /translation="MREEVVAVFIPIIMTLVIGSVFIIYFYLKSKEKQMLIEKGLSAE
                     EIKKFFEEKRDGLWLMKIGIISIFFGLGLGFGMMLQDATAKEYWIPFFLFVGTGIGFV
                     IANVVADKLKKKNN"
     gene            1868..2434
                     /locus_tag="VF870_12020"
     CDS             1868..2434
                     /locus_tag="VF870_12020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9252963.1"
                     /translation="MKNLTDVEIIESVKKGNQADYAVLIDRYKNKAFTMLKRMLKNES
                     DAEEVLMDCFLKAYNNLNTFKFESKFSTWFYRIVYNTALTKISSAKRKIESEMSSIDD
                     LHYLESNYNADDLVKEDLAILVKKIVNELPPQNAAVVTMFYLEEMNTEEISEVLKISV
                     SNVKVILYRSRNLLKDIIANRNLLQEIL"
     gene            2431..2940
                     /locus_tag="VF870_12025"
     CDS             2431..2940
                     /locus_tag="VF870_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252964.1"
                     /translation="MKTIDDEMLNRYIDNELSIEDRNFVNNAIENSSDVKKRYEFLLK
                     AHLLLKNIQPESPSMDFSKLVMQKISRRGIQESQQKRFLFAVLSLFGIVILGLVGYVF
                     YEIISSIQSSDSSAAISTYSNTIGDYFSALFGKKNLSIFGSVLSFIMLISGYFLYNFQ
                     KHSKNNFSH"
BASE COUNT         1075 a          455 c          528 g          997 t
ORIGIN      
        1 tttgataact ggatttttgg ttgtgatatt tgtcaggatg tttgtccatg gaatcaaaag
       61 ttttctcaac agacctggat accagaattc tatccataca ataaagaatt taattataat
      121 gaaattatga aacttgatga acaatcattc aaacagcgat ttgcagaaag cccgataaaa
      181 agaactaaac taaaaggtct gcagcgtaat gctaagtttt tgtttgaatg atcagtcaac
      241 cttcttgaat cttcattaac ttatctgaat cttacaagta gaaaaaatat aaatttagct
      301 cataatcagt tttggagaaa aaatgtctga acaagcaaat caaaaaaatc attttaaagt
      361 tttaattatt ggttccggtc ccgctggatt gacggccgca atttatactg ggcgtgctaa
      421 tcttagtcca gtaatatttg aaggaatgca gccgggcgga caacttacta taacaactga
      481 agttgaaaat tatccaggct ttgaacacgg aattgatggt ccggcattaa tggacgttat
      541 gcgtaaacag gcgcaaagat ttggagcgca atcattttac aaggaagtaa cagaagttga
      601 tttttcaagc cgtcctttca aagtaaaatc atacgatgaa aattacactg ctgattccat
      661 cattatttca acgggagctt ctgcaaaatt gcttggatta gaaagtgaaa aaaaatatat
      721 gggttatggt gtttcagctt gtgcaacctg tgatggcttt ttctttaaag gattaaaaat
      781 aattgttgtt ggcggcggag atacagccat ggaagaagca aacttcttaa caaaatttgc
      841 tgatgaagtc actatcattc atagaagaga tgaatttcgt gcatcgaaaa ttatgaccga
      901 acgggcacag aaaaatccaa agataaaatt tatgacaaat aaaatagtta aagaagttat
      961 tggtgttgaa gagaatggaa agaaaagtat gacgggagtt attcttgaag acacaaaaga
     1021 tcattctttg acggaattta aagccgatgg aatgtttctt ggaattggtc atcaaccaaa
     1081 tactaagtta ttcaaaggtt ttctagatat ggatgaaacc ggttatttga taactaaacc
     1141 tggttctact tatacgaaca ttgaaggtgt atttgcggct ggagatgttg ctgatcatgt
     1201 ttatcgacag gcggttaccg ctgccggtac aggttgtatg gctgctttag atgccgagag
     1261 atggttggag acacaggaag cctaatccca aaaacccctt ccattaggga aggggttttt
     1321 taattaatta ttttttttct tcaacttatc agcaacaaca tttgcaatca caaatccaat
     1381 accggttcca acaaataaaa agaatggaat ccaatattct ttagcggttg catcctgtag
     1441 catcattccg aatcctaagc ctaagccgaa gaaaattgaa attattccga tcttcattaa
     1501 ccagagccca tcgcgttttt cttcaaagaa ttttttgatt tcttcagcag agagaccttt
     1561 ctctattaac atttgctttt ctttagattt taaataaaag taaatgataa atactgagcc
     1621 aattacaagt gtcattataa ttggaataaa tacagctaca acttcttcac gcattttagt
     1681 tctcctttgt ttctaatcct taagacaaaa ggatttgatt attggttaca agattattcc
     1741 atttgctata tttgacttaa gaaatcaatt tgaggttaca aattgattct ataaagattt
     1801 caaactaaat tactaccgca tatagatatt tgtaaccttt ggtatagtgt attagtctat
     1861 tatgataatg aaaaacctta ccgatgttga aattattgaa tccgtaaaga aaggtaatca
     1921 agcggattat gctgttctta ttgatcgtta taagaataaa gcttttacca tgctaaaacg
     1981 tatgctaaaa aatgaatctg atgcggaaga agttttaatg gattgttttt taaaagctta
     2041 taacaatttg aatactttta aatttgaatc aaagttttca acttggtttt accggatagt
     2101 ctataataca gcactgacaa aaatatccag tgcaaaaaga aaaattgaaa gtgaaatgag
     2161 ctctatagat gatttacatt atctggaaag caattacaat gctgatgatt tagttaaaga
     2221 agatttggca attcttgtaa agaaaattgt aaatgaactt ccaccgcaaa atgctgccgt
     2281 agtaacaatg ttttatcttg aagaaatgaa tacggaggag atcagcgaag tgttaaagat
     2341 ctccgtgtct aatgttaaag taattctgta tcgctcaaga aatcttttaa aagatattat
     2401 tgcaaataga aatttattgc aggaaatttt atgaaaacta tagatgatga aatgttaaat
     2461 agatatatcg ataatgaact tagtattgaa gatagaaatt ttgtaaacaa cgccattgaa
     2521 aactcttctg atgttaaaaa aaggtatgaa tttttattaa aagctcattt acttttaaaa
     2581 aatattcaac cagaatctcc atcaatggat ttcagcaaac ttgtaatgca aaaaatttcc
     2641 agacgtggaa ttcaggaatc tcaacagaaa agatttttat ttgcagtctt atcattattc
     2701 ggaatagtta ttctcggttt agttggatat gttttttatg aaatcataag ttcaattcaa
     2761 tcaagtgatt cgagtgccgc aatttcaact tattcaaata caattggcga ttatttctca
     2821 gctctctttg gcaaaaaaaa tctctcaatt ttcggttcag tgctttcttt tattatgttg
     2881 atatcaggat actttttata caactttcaa aaacattcaa aaaataattt tagtcattga
     2941 aatctatagt cacattaagc gaagtcaaaa tgtgatagac tttaattcag attaaaaact
     3001 atcttacgac ttcagtaaag atgacattga gtttcttatc tgcaatctac cttaa
//
LOCUS       DASWBY010000659         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_62720, whole genome shotgun sequence.
ACCESSION   DASWBY010000659 DASWBY010000000
VERSION     DASWBY010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62720"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..2071)
                     /locus_tag="VF870_12030"
     CDS             complement(377..2071)
                     /locus_tag="VF870_12030"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9252965.1"
                     /translation="MNVNTKFILSLLVLLTISNYAQYDLEAAFPNLTFSSPLNLQNAG
                     DGTNRLFVVEQNGVIKVFQNLSNVTASKTFLNITDRVTSGGETGLLGLAFHPDYENNG
                     YFYVNYTAPNPLRTVISRFQVSSTNPDSADKNSELIILTYDQPFSNHNGGCTAFGPDG
                     FLYISAGDGGSGGDPQNNAQNITNLLGKIIRIDVDNPQTPLNYGIPSDNPFVDSTNVN
                     IRKEIYAWGLRNTWKFSFDSVSGWLWAADVGQGSWEEVDIIQNGKNYGWRCYEGNHPY
                     DLSGCNGTYEFPIWEYSHSVGFSITGGYVYRGQNVPELYGKYIFGDYVTARVWALDYD
                     GINPATSTQITTAPGSITSFGEDENKELYLVSFNGKIYRFIPTIVPVELLSFNATVQD
                     GKVFLQWYTSTETNNAGFKIERSKNGADYEEIFFVGGNGTTTNRNVYSYLDESVHSGV
                     YYYRLKQIDYNGTFENLNVIAVDLGIPGGFLLEQNYPNPFNPKTVINFQTPKEGLVIL
                     KVYDLLGNEISTLVNEQKPAGSYSVDFDASKLTSGMYLYKLISGSFSYTKKMILLR"
     gene            complement(2107..>2768)
                     /locus_tag="VF870_12035"
     CDS             complement(2107..>2768)
                     /locus_tag="VF870_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252966.1"
                     /translation="RKKILSFLLLNRILFQILKSIEITDSKIKLFDELLLWKPLFEIY
                     GFLRYDDPGKSHELIKSLFDSNISFNENVDRYFIKLFDKDITASFVQVNEYENTKYFN
                     KERIEELVKWHFILACSNLISDTVPIAEISKTFLKDSLKLMFENYSAIFQKILKSEYK
                     VENIISIPEVKIIKEVKAEKKPAVKKTATKKITQKKKTTNKLKTKKTDKAKKKVKPKR
                     K"
BASE COUNT          909 a          473 c          394 g          992 t
ORIGIN      
        1 tctttaataa cttcattttg ttttttacca atcatatcgt gcaccgcgat attttgtgca
       61 ccaataaagt tcgaaaaaaa taaaatagaa aataagaatt gaatagatac agtagacttt
      121 ttcataatta cctcactaat gacttagtaa atatttaatt aatgaaattg ctttggattg
      181 gttatagaat tgggaattct tgcaaaaaga atttcgaaaa gtaagccaac tgaaataaat
      241 gatggacatt taaaaatcag ctaaaaacct taattaattt tactttattt aattatgatt
      301 gtcaatcgat ttagtccaaa aaaaataggc tccttaagag cctattacta aaatatttaa
      361 attaactata aaaatattat ctcaacaaaa tcattttttt agtatagcta aaagatcctg
      421 aaattaattt atataagtac attccactgg ttaactttga tgcatcaaaa tcaacgctat
      481 aacttccagc aggtttttgc tcgttaacta atgtggaaat ttcatttcca agcaaatcat
      541 atacttttaa tataacgaga ccttctttgg gagtttgaaa attaataact gttttaggat
      601 tgaatggatt tggataattc tgttccagta aaaatccacc cggaatccct aaatcaacag
      661 caataacatt taaattttca aacgtaccat tatagtcaat ttgttttagt cgatagtaat
      721 aaactccgga gtgaaccgat tcatctaaat agctgtatac atttctgtta gttgtagtcc
      781 catttccgcc gacaaaaaat atctcttcat aatctgctcc gttcttactt ctttcgattt
      841 tgaaccctgc attgtttgtt tcagtagagg tataccattg aagaaagact tttccatctt
      901 gtacagtagc attaaaagat agtaactcta ctggaacaat ggttggaata aatctgtaaa
      961 tctttccatt aaatgatacc aggtacaatt ctttattctc atcctcacca aacgaagtga
     1021 ttgagcccgg agcagttgtt atttgagtac ttgttgccgg atttattcca tcgtaatcta
     1081 atgcccaaac tcgcgctgtt acataatcac caaaaatata ttttccgtaa agttccggta
     1141 cattttgccc gcgataaacg tatccaccag taattgaaaa tccgacggag tgtgaatatt
     1201 cccaaatcgg aaattcgtaa gtcccattgc atccgcttaa atcataagga tgatttcctt
     1261 cataacatct ccatccataa ttttttccat tctgaattat atcaacctct tcccatgaac
     1321 cttgtccaac atcggcagcc caaagccatc ctgaaaccga atcaaaacta aatttccagg
     1381 tatttcgtaa accccaggca taaatttctt tacgaatatt tacattcgta ctatcaacaa
     1441 aaggattatc tgatggtatt ccgtaattaa gaggcgtctg cgggttatca acatcaatac
     1501 gaattatttt accaagtaga ttggtaatgt tttgtgcatt gttttgcgga tcaccaccag
     1561 agcctccgtc acctgcggat atatataaaa atccatcagg gccaaatgct gtacatcctc
     1621 cattatgatt tgaaaatggc tgatcataag taagtataat taattctgaa tttttatctg
     1681 ctgaatctgg gtttgtggat gaaacctgaa atcgtgaaat aacagtgcgc aatggatttg
     1741 gggcagtgta gttaacgtaa aagtaaccat tgttttcata atccggatga aaagccaaac
     1801 caagtaatcc cgtttcgcct ccggatgtaa cacgatcagt aatgtttaaa aaagtttttg
     1861 atgcagtgac gtttgataga ttttggaata ctttgataac accattttgt tcaacaacaa
     1921 ataatctgtt tgtgccatca ccagcatttt gaagatttag cggacttgag aacgtcaggt
     1981 tgggaaatgc agcttccaaa tcatactgtg cataatttga tattgttaat aataccaata
     2041 gtgaaagtat aaattttgta tttacattca tgtttttgcc tccttataaa tctaaatatt
     2101 cagttattat tttctttttg gtttaacttt tttcttcgcc ttatctgttt tcttagtttt
     2161 taatttatta gtcgttttct ttttttgcgt aattttcttt gttgctgttt tctttactgc
     2221 aggcttcttt tctgctttta cttcctttat gattttaacc tctggaattg agattatatt
     2281 ttcaacctta tattcacttt ttaatatctt ttgaaaaatt gcagaataat tttcaaacat
     2341 aagcttcaaa gaatctttta aaaatgtttt agaaatctcg gctataggca cagtatcaga
     2401 aatcaaattt gagcacgcga gaataaaatg ccatttaact agttcttcga ttctttcttt
     2461 gttaaagtat tttgtattct catattcatt tacctgaacg aatgaagcag taatatcttt
     2521 atcaaataat tttatgaagt atctatcaac gttttcattg aaagaaatat tagaatcaaa
     2581 taaactttta attaattcat gacttttacc aggatcatca tatcttaaga aaccgtaaat
     2641 ctcaaacaat ggtttccaca ataaaagttc atcaaataat ttgatttttg aatcagtaat
     2701 ctcgattgat tttaatattt gaaatagaat tctatttaac aatagaaaag ataatatctt
     2761 ctttcttt
//
LOCUS       DASWBY010000660         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_663358, whole genome shotgun sequence.
ACCESSION   DASWBY010000660 DASWBY010000000
VERSION     DASWBY010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663358"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VF870_12040"
     CDS             <1..903
                     /locus_tag="VF870_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9252967.1"
                     /translation="DPFFKQFFGNRGNSSQKVQGLGSGYIISPDGYIVTNDHVAGNAS
                     EITITMTDGSHHEAKIVGSDPVSDICLLKVDSNNLPYVELGNSSDIMIGEWAIALGNP
                     FGLFELNDKPTVTVGVISATGMNLEPINDRYYLNMIQTDAAINGGNSGGALVNSLGQV
                     IGMNTLIYTAGGVQGNIGLGFAIPIDKVKRVVTELKANGKIDRDFQIGMSIQSIDAGI
                     MRYYNLKSNKGVIITKVLPNTPADNAGLKSGDIITEIEGYKISNEQTIFGVFQEFRTG
                     QEIKVKIIRDNVELVKNMKLEKNK"
     gene            900..>2081
                     /gene="purB"
                     /locus_tag="VF870_12045"
     CDS             900..>2081
                     /gene="purB"
                     /locus_tag="VF870_12045"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9252968.1"
                     /translation="MIERYTLPEMGKIWEDKFKFETWLRIEILACEARAEMGEIPKQD
                     VEIIKQKASFDVKKILEIEETTKHDVIAFLTNVAEYVGPESRHIHYGMTSSDILDTTL
                     SYQMKSAGELLLKRLVDLKDALKLRAIEHKNTVCVGRSHGIHAEPTSMGLKFALWFEE
                     TKRNIQRLQKAIDVISFGQISGAVGTFEHLSPKVEEYVCNKMGLKPAPVSTQVIQRDR
                     HAEFLTTLAIIGATLEKISIEIRHLQRTEVLEAEEYFSKGQKGSSAMPHKRNPIISER
                     VTGLARVLRSNSMSALENVALWHERDISHSSVERIIVPDSCIALDYMLDLMVKLISKL
                     IIYPENMIRNLNLTRGLIFSQTILLKLVTKGISREDAYRIVQDSAMKVWADEKLNLKD
                     EL"
BASE COUNT          733 a          305 c          433 g          610 t
ORIGIN      
        1 gatcctttct tcaaacaatt ttttggcaac aggggcaatt ccagtcaaaa agttcaggga
       61 ttaggatctg gatatattat ctcacctgat ggatatattg ttacaaacga tcatgttgct
      121 ggtaacgctt ctgaaattac aataacaatg actgatggaa gtcaccacga agctaaaatt
      181 gttggatccg atcctgtatc ggatatctgc ttgcttaagg tagatagtaa taatctgcct
      241 tatgtagaat tagggaattc aagtgacata atgatcggag aatgggcaat agcattaggt
      301 aacccatttg gattgtttga acttaatgat aaaccaactg ttacggttgg tgttatcagt
      361 gcaacaggaa tgaatcttga accaatcaat gatcgctatt acttaaacat gatacaaaca
      421 gatgctgcaa ttaatggtgg caacagcggt ggtgcattgg ttaattcgct gggacaggtt
      481 attggaatga atactctgat ttatactgcc ggaggtgttc agggaaatat tggacttggt
      541 ttcgcaatac ctattgataa agtaaaaagg gtcgttactg aactaaaagc aaatggtaaa
      601 atagatagag atttccagat tggaatgagc atacaatcta ttgatgcagg tattatgagg
      661 tattataatc ttaaaagtaa taaaggtgtt ataatcacca aagtattacc aaatactccc
      721 gctgataatg ccggtctaaa aagtggagat ataattacag aaattgaagg ctataagatt
      781 agtaatgaac aaactatttt tggagtgttc caggaattta gaaccggaca ggaaataaaa
      841 gttaaaataa tacgagataa tgttgaatta gtaaaaaaca tgaaattaga gaaaaataaa
      901 tgattgaaag atatactctt cccgagatgg gaaagatttg ggaagataaa ttcaagtttg
      961 aaacatggtt aaggattgaa atccttgctt gtgaagcacg tgcagaaatg ggtgaaattc
     1021 caaaacaaga tgttgaaata ataaaacaaa aagccagttt tgatgtaaag aaaattctgg
     1081 aaattgaaga aacaacaaaa catgatgtta ttgcattctt gactaatgtt gcagagtatg
     1141 tgggcccgga atcaaggcac atacattacg gtatgacttc ttcagatatt ttagatacta
     1201 ctttatcgta tcaaatgaag tctgcaggag aactgcttct gaaaagatta gttgatctga
     1261 aggatgcgtt aaaattaaga gctatcgaac ataaaaatac tgtttgtgtc ggcagatcgc
     1321 atggaataca tgcagagccc acatctatgg gattaaaatt tgcattatgg tttgaggaga
     1381 caaaaagaaa tatccaaaga ctacaaaaag ctattgatgt tataagtttt ggccaaatat
     1441 caggcgccgt tggaacattt gaacaccttt ctccaaaagt tgaagaatac gtttgtaata
     1501 aaatgggttt gaagccagct ccggtttcaa ctcaagttat tcagagggat cgacacgctg
     1561 agttcttaac aacacttgct attatcggag caacattaga aaaaatttct attgagatcc
     1621 gtcatctcca gaggacagaa gttcttgaag cagaagaata tttttcaaaa ggacaaaaag
     1681 gttcatctgc tatgccccat aagcgtaatc ctattatcag tgagcgtgtt actggattag
     1741 ctagagtgct cagaagcaac tcaatgtcag cattagaaaa tgtggctttg tggcatgaac
     1801 gtgatatttc gcattcttca gttgaacgaa tcatcgtgcc cgatagttgt attgcactag
     1861 attatatgct cgatttaatg gttaagctaa tttctaaact tattatctat cctgaaaata
     1921 tgattagaaa tttgaacctt actcgtggac taattttttc gcagacaatt ttattaaaac
     1981 ttgtaactaa aggaatttcg agagaagatg cttatagaat tgtacaagat tcagccatga
     2041 aagtctgggc tgatgaaaaa ttgaatctga aggatgagtt a
//
LOCUS       DASWBY010000661         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_325186, whole genome shotgun sequence.
ACCESSION   DASWBY010000661 DASWBY010000000
VERSION     DASWBY010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325186"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VF870_12050"
     CDS             <1..550
                     /locus_tag="VF870_12050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9252969.1"
                     /translation="RPFGIDTKNLISEITFDKSDNDASKKFISGIKQDSKSIIVGLHV
                     GAGKPQNRWSLTKYVELIHKLKNEYDCVFYLTGSDSDVEDLNFVTDNSRIKLHKYLNK
                     QIPQVAALIANSDLFITNDTGIMHVAGTTTTPQISLFGPTNPFNWAPCGKGKIFLRKS
                     DLIDDIEVEDVFAACKNLLESK"
     gene            547..1506
                     /locus_tag="VF870_12055"
     CDS             547..1506
                     /locus_tag="VF870_12055"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEX9252970.1"
                     /translation="MSLPDKNRIAAIDIGTNSFHLIIVEVKKNGGLKLLDRKREFLRL
                     GSEIGEDLSFISENEKTKAISVLKNFSNLAQFHRASLRAVSTSAVREAKNKNEFIKAV
                     LDETGIKVETIEGTEEAKLIYLGMKNALPLNDKSVLGIDIGGGSTEFIYGVNGTAVFA
                     ESVKIGAVRLSKKFFPDFIITESSVNDCTEYVENQIKIKNRIRTDIKLDFAVGSSGTI
                     DTICLIKQSQKEAKIKPRLNGYTFNKSEFDEIYSYVMSLKNPDERISVPGIEAKRADI
                     IPAGFIILKKVLEIFNIEKMVLSEYALREGVVFDLINKIKLSN"
     gene            1586..>2256
                     /locus_tag="VF870_12060"
     CDS             1586..>2256
                     /locus_tag="VF870_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9252971.1"
                     /translation="MNNIRNFCIIAHIDHGKSTIADCLLETTGVVSAREAKDQILDDL
                     ELERERGITIKSHAIQMHYRAKDKQEYVLNLIDTPGHVDFTYEVSRSLAACEGAILVV
                     DAAQGVEAQTISNLYLATDAGLEIIPVINKIDLPSAEIDKVSNQIIDLIGCNKEDIIL
                     VSAKSKIGIVDLLETVIEKIPSPKGDPNAPLQALIFDSVFDAYRGAIAYVRIFQGTLK
                     TNDKL"
BASE COUNT          809 a          333 c          408 g          706 t
ORIGIN      
        1 aaggcctttt gggattgaca ccaaaaatct aatctctgaa ataacttttg ataaatctga
       61 taatgatgct tccaaaaaat ttatttcagg aataaagcag gattcaaaat caattatagt
      121 tggtttacat gttggtgctg gtaaacctca aaacagatgg tctttaacaa aatatgttga
      181 gttaatacat aaattaaaaa acgaatatga ttgtgttttt tatttaacgg gatcagattc
      241 agatgttgaa gatttaaatt ttgtaactga taatagcaga ataaaactgc ataaatattt
      301 aaataaacaa attccacaag ttgcggctct tattgcaaac tccgatctat ttattacaaa
      361 cgataccgga attatgcacg ttgccggcac aactactact ccgcaaattt ctttatttgg
      421 accgacaaat ccttttaatt gggctccttg cggcaagggt aaaatatttt tacgtaaatc
      481 agatttgatt gatgatattg aggttgaaga tgtttttgct gcttgtaaaa atctacttga
      541 aagtaaatga gcttacctga taaaaacaga attgctgcta tagatatagg cacaaactcc
      601 tttcacctta ttattgttga agttaaaaaa aatggcggct taaaactttt agaccgaaaa
      661 agagaatttt taagattagg ttcagagatt ggagaagatt taagttttat ttctgaaaac
      721 gaaaaaacaa aagcaatttc agtattaaaa aatttttcaa atctggcaca atttcataga
      781 gccagcctta gagctgtttc cacgagcgca gtgcgagaag ctaagaataa gaacgagttt
      841 atcaaagcag tgcttgatga aaccgggatt aaggttgaaa cgattgaagg aactgaagaa
      901 gctaaactta tttatcttgg gatgaaaaat gctctgccgt taaatgataa atctgttttg
      961 ggaattgata ttggaggcgg aagcactgaa tttatttacg gagtaaatgg tacagcagtt
     1021 tttgcagaaa gtgtaaaaat cggcgcagtc agattatcta aaaaattctt tcctgatttt
     1081 ataattactg aatcgtctgt taatgattgc actgaatatg ttgaaaatca gataaaaata
     1141 aaaaatagaa ttagaacgga tattaaactt gattttgcag ttggttcatc cggtacgatt
     1201 gatacaattt gtcttattaa acaatctcag aaagaagcta aaatcaaacc caggttaaac
     1261 ggatacacat ttaataaatc tgaatttgat gaaatatatt cttatgtgat gagcttaaaa
     1321 aatcctgatg agagaataag tgttccggga atagaggcca agcgtgccga tattattccc
     1381 gcaggtttta tcattctaaa aaaagtacta gaaatattta acattgaaaa aatggtttta
     1441 tcagaatatg cacttcgtga aggtgttgta tttgatctta taaacaagat aaaattgtct
     1501 aactgaactg tctttatcac aaaaacacta atctttttta atacttacta cctgactata
     1561 tttgcacact aactattaag agaatatgaa taacattaga aatttttgca taattgcaca
     1621 cattgatcat ggtaaatcaa ctatagcaga ttgtctttta gaaaccactg gggtcgtatc
     1681 tgcacgagaa gcaaaagacc aaatattaga cgatcttgaa cttgaacgtg aacgtggtat
     1741 aactataaaa tctcatgcaa ttcaaatgca ctaccgtgca aaagataaac aggaatatgt
     1801 gcttaattta attgatacac ctggtcacgt ggattttaca tatgaagttt cccgatcact
     1861 tgcagcttgc gaaggagcaa ttcttgttgt tgacgctgcg cagggagttg aagcccagac
     1921 tatcagtaat ctttatctgg ctactgatgc cggattagaa attattccgg ttattaataa
     1981 aatagatttg ccaagtgctg agattgataa agtgtcaaat cagattattg atttaattgg
     2041 ctgcaataaa gaagatataa tcttggttag tgcaaaatct aaaattggaa ttgttgatct
     2101 tttagaaact gttatagaaa aaattccttc accaaagggt gatccaaatg caccattaca
     2161 agcgctgata tttgattctg tattcgatgc ataccgcgga gcaatagctt atgtcagaat
     2221 ttttcaaggc actttaaaga ctaacgacaa actaag
//
LOCUS       DASWBY010000662         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_441049, whole genome shotgun sequence.
ACCESSION   DASWBY010000662 DASWBY010000000
VERSION     DASWBY010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441049"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF870_12065"
     CDS             <1..213
                     /locus_tag="VF870_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252972.1"
                     /translation="KKAKFEKSLIADDKLISKVKVSKEINGAKIESTGYYQTNEITIT
                     LYRSKDSLLKDGYIKKGDLETYSEDE"
     gene            306..1400
                     /locus_tag="VF870_12070"
     CDS             306..1400
                     /locus_tag="VF870_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9252973.1"
                     /translation="MNNQRLIDLFLEVAKINSLSGNEKPIADYIKTFLSVLGYKVNED
                     NSREFTNSNTGNLICKIGSGGNFIMLSHMDTARPTENVRPIILEDKIISSGDTVLGVD
                     NRAGISVLLYSLEKIAKEKIPVKDFTVAFTTCEETTLFGSKNLGINGEIKNGFIFDSG
                     YRPGNFIYSACGAIGLKIKIFGKASHSGISPEKGINSMQAAAKAISQLPLGRIDDETT
                     MNIGTLKSGSAVNVIPELTELEGEVRSFNPKKAEDNFNLLVNIFKSEAEKIGAKVEID
                     YFWDFVPYTIKEDSFVFQETVRAIKNVGLEPSPKISLGGSDANSLNGRGIESINLGIG
                     AQNPHSNDEFIFIEDLIKSAEIALELVKKD"
     gene            1406..2260
                     /locus_tag="VF870_12075"
     CDS             1406..2260
                     /locus_tag="VF870_12075"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX9252974.1"
                     /translation="MKKLFYIVLVLLLVFSSDFYSQSKGKLLIVGGAQTHEIEKKFVE
                     LAGGPDARIIIIPNAGSEPKKYSEIEQKTFNELGAKADYILFTRETADDEANLKKIDW
                     ANAVFFTGGDQSDLTRDMLGTKLLQKVFDIYNNGGTVGGTSAGAAVMSEVMITGNELL
                     HNDSTGSFITIEKNNIETKQGFGFLKTVIIDQHFLKRKRHNRTISALIEHPNLLGVAI
                     DESTSIIVYPDETFEVFGSNQVLVYDPTSATNIREDFKGNLGINNMKLHVLINGDKFD
                     LKSKEVIE"
     gene            2257..>2981
                     /locus_tag="VF870_12080"
     CDS             2257..>2981
                     /locus_tag="VF870_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEX9252975.1"
                     /translation="MTEAKKKFKFRVPNTYLLIFSLLVLIAALTWIIPGGQYERAVVN
                     GREVVVQNSFKYVESHPQGFIDLFIAPLKGFVEAGLIIGFILFVGGSFNVLAKTEAIN
                     SLIHKLARAHKDSKLLQKLFIPVLMLMFSLGGATFGMNEEIIPFVLIIVPICLALGYD
                     SIVGVAIPLVGAHIGFASAFLNPFNVGIAQGIAEVPLFSGIGYRIISWAISTSVAILF
                     LLWYVKRLSKNPKVSPTYLQDEER"
BASE COUNT          993 a          441 c          581 g          966 t
ORIGIN      
        1 aaaaaggcaa agtttgaaaa atccttaata gcagatgaca agctgatatc aaaagttaaa
       61 gtaagtaaag aaattaatgg tgctaagatt gagtctacag gttattatca gacgaatgag
      121 atcactataa cattatatag atctaaagat agtttgctta aagatggata catcaaaaag
      181 ggcgatttag aaacttactc tgaagacgaa taattttttg tatttcttct tgacttaagt
      241 aaatattttt tgtaactctg taatagttat tacagctatt aataaatttg taattagagt
      301 tacagatgaa caatcaaaga ttaatagatc ttttcttaga agttgccaaa atcaactctc
      361 tctccggcaa tgaaaaacct atcgcagact atatcaaaac ttttctgtca gtacttggat
      421 acaaggttaa tgaagataat tcaagagaat ttaccaacag taatacgggc aatctgattt
      481 gcaaaattgg cagtggtgga aattttataa tgttatcaca catggatact gcacgcccaa
      541 cagaaaatgt tagacctatt attcttgaag ataaaataat ttcctcaggt gatactgtgc
      601 ttggtgttga taacagagcc ggtatttctg ttttgcttta ttctcttgaa aaaattgcaa
      661 aggaaaaaat tcctgttaag gattttacag ttgcatttac tacgtgtgaa gaaactacat
      721 tgtttggatc aaaaaatctg ggaattaatg gtgagataaa aaatggattt atttttgatt
      781 caggttatcg cccaggaaat tttatttact ctgcctgcgg tgcgatagga ttaaagataa
      841 aaatatttgg taaagcttcg cactccggaa tctctcctga aaaaggcatc aactcaatgc
      901 aagcagctgc taaagcaatc agccaacttc cgcttggtag aattgatgat gaaactacaa
      961 tgaacatcgg tacacttaaa agcggctctg ccgtaaatgt tattccggaa ctgacagagc
     1021 ttgaaggaga ggttagatca tttaatccga aaaaagcaga agacaacttt aacttgttag
     1081 tgaacatctt taagagtgag gctgaaaaaa tcggggctaa agttgaaata gattactttt
     1141 gggattttgt tccatatacg attaaagaag attcttttgt ttttcaggaa acagttagag
     1201 caataaaaaa tgttggttta gaaccatctc ctaaaatttc acttggcgga agcgatgcga
     1261 attcacttaa tggacgcggt atcgaatcaa ttaatcttgg tatcggtgct caaaaccctc
     1321 attcaaatga tgagtttatt tttattgagg atttaattaa atcagctgaa attgcactag
     1381 aactagttaa gaaggattaa tcaatatgaa aaaacttttt tacattgtac ttgttttact
     1441 tttagtgttt agttctgatt tttattctca atcaaagggt aagttactta ttgttggcgg
     1501 tgcccaaact cacgaaatag aaaaaaagtt tgtagaactt gccggtggac ctgatgcacg
     1561 gattataatc attccaaatg caggctccga accaaaaaaa tatagtgaga tagaacaaaa
     1621 aacatttaat gaattaggtg caaaggcaga ttacatttta tttacccggg aaactgctga
     1681 tgatgaagcg aatttaaaga aaattgactg ggccaatgct gtttttttta ccggtggtga
     1741 tcagagtgat ttgacacgtg atatgcttgg aacaaaacta cttcaaaaag tttttgatat
     1801 ttataacaat ggcggaaccg ttggcggtac aagtgctggt gccgcggtta tgagtgaagt
     1861 tatgattacg ggcaatgagt tacttcataa tgattcaaca ggttcattta taacaatcga
     1921 aaaaaataat attgaaacca aacaagggtt tggtttttta aagaccgtaa taattgacca
     1981 gcattttctc aaacgtaaaa ggcataaccg aactatttca gctttaattg aacatcctaa
     2041 ccttttaggt gttgcaatag atgaatcaac ctcaataatt gtatatcctg atgagacttt
     2101 tgaagttttt ggcagtaacc aggttttagt ttatgatccg acaagcgcaa ctaatataag
     2161 agaagatttt aaaggaaatc ttggaatcaa caatatgaaa ttacacgttt tgattaacgg
     2221 tgataagttt gatcttaagt caaaagaagt aattgaatga ctgaagcgaa aaagaaattt
     2281 aaatttcgtg ttccaaacac gtaccttttg attttttctt tgctggtttt aattgcggct
     2341 ttgacgtgga ttattccggg cggacaatat gaacgagcgg ttgttaatgg aagagaagtt
     2401 gttgtacaga actcatttaa atatgtagaa agtcatccac aaggttttat tgatctgttt
     2461 attgctcctt taaagggatt tgttgaagca ggattaataa tagggtttat attatttgtc
     2521 ggcggttctt ttaatgtgct tgcaaaaacc gaagcaatta attccctgat ccataaactt
     2581 gcaagagcgc ataaagattc taagttgctt caaaaacttt ttattcctgt gcttatgctc
     2641 atgttttcac ttggcggtgc gacatttggg atgaatgaag aaattattcc atttgtatta
     2701 ataatagttc caatttgcct ggcattagga tatgattcaa tagttggtgt tgctattcct
     2761 ttggttggtg cgcacattgg atttgcaagt gcatttctaa atccgtttaa cgttggaatt
     2821 gcacagggca tcgcagaggt tcctttattc tctggtatcg gttatagaat tattagctgg
     2881 gctatctcta catctgtggc gattttgttt ttattatggt atgtaaaaag gctttcaaaa
     2941 aatcctaaag ttagcccaac ttatcttcaa gacgaagaac g
//
LOCUS       DASWBY010000663         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_441170, whole genome shotgun sequence.
ACCESSION   DASWBY010000663 DASWBY010000000
VERSION     DASWBY010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441170"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VF870_12085"
     CDS             <1..870
                     /locus_tag="VF870_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9252976.1"
                     /translation="LAYSTLSVLGTLTMLLGIGSNLAIKAFFIFMVAHSLYKGTLFLV
                     AGTIDHETGTRVVTTLGGLKKYLPVTMVAALLASFSMMGIIPLVGFIGKETVYSAILE
                     VENFRFLILALAVISNAFVVMVTILVGLKPFFGNILPTPKSPHEAPLKMLIGPIILAA
                     FGLLSGLFPNVFIQKLLDQSTISIISEELGIEVKLWHGFNFVLLLSFVTLALGISIYY
                     FRKQIIQALANISLTNFIKPSYWYERIFNGTLSFGKFQTKILQNGYLRYYIIFIILTT
                     LGLAEYALFQIPL"
     gene            867..1412
                     /gene="mbhE"
                     /locus_tag="VF870_12090"
     CDS             867..1412
                     /gene="mbhE"
                     /locus_tag="VF870_12090"
                     /inference="COORDINATES: protein motif:HMM:NF042818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HEX9252977.1"
                     /translation="MSSLNIDTDITIYELIIGIIIIASTLLVITSDSRLKAIVSLGVV
                     GFAMGIIFILYSAPDLALTTFAIETLTVILFVLVLYKLPKYQKFSKPGSKIRDAIIAG
                     SVGVFMMLVVLLVTAGEMQSELKKYFAEASLPEGKGRNIVNVILVDFRALDTLGEITV
                     LAVAAIGVYALLKLRLKEGEK"
     gene            1412..1828
                     /locus_tag="VF870_12095"
     CDS             1412..1828
                     /locus_tag="VF870_12095"
                     /inference="COORDINATES: protein motif:HMM:NF009163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit B"
                     /protein_id="HEX9252978.1"
                     /translation="MYSVILSTASRYLFPLMLIFSFFLFLRGHNEPGGGFVGGLVAAA
                     AYALYLIANGVEEAKKLLKAEPIKLIAIGLLLAIISAFISVIIGQDFMTGLWAKNEFP
                     VIGKVGTPLLFDLGVYFLVLGIVTKIIFSLAEEDKQ"
     gene            1825..2190
                     /locus_tag="VF870_12100"
     CDS             1825..2190
                     /locus_tag="VF870_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HEX9252979.1"
                     /translation="MTFLLAILVGVLYSAGIYMILRRSLVKVIIGLIFLGHAANLLIF
                     TIGRVTKGIPAFVPKGSEILAEPFADPLPQALILTAIVIGFGVQAFAIVLFKRAYQTV
                     ETDDLDKMKSTDELKEDKQ"
     gene            2187..>2981
                     /locus_tag="VF870_12105"
     CDS             2187..>2981
                     /locus_tag="VF870_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9252980.1"
                     /translation="MNLIIVLPIIIPLLFAVIMLFFWKNVNAHKILNVIASLFSLAVS
                     IILLSDVNASGIISLQVGSWQAPFGISLVADLLASIMVLITAVIGLAASIYSLGLMDE
                     QREKFLYYPLLQFLLMGINGAFLTGDIFNLYVWFEVMLISSFVLLALGGKREQLEGAI
                     KYVTLNLLSSAIFLAAVGILYGIVGTLNMADLARKISLVQQKELVTVVSILFMIAFGI
                     KAAVFPLFFWLPASYHTPPIAVSAIFAGLLTKVGVYALIRIFTLIFV"
BASE COUNT          844 a          518 c          559 g         1060 t
ORIGIN      
        1 ctggcttact caactttaag tgttcttgga acattaacta tgcttttggg aatcggttca
       61 aatctcgcta taaaagcttt cttcattttt atggttgcgc attcattata taaaggaact
      121 ttatttcttg ttgccggaac tattgaccac gaaacaggaa caagagttgt tacgacactt
      181 ggcggcttga aaaaatatct gcctgttaca atggttgcgg ctctgttggc atcattttct
      241 atgatgggga taattcctct tgttggtttt attggcaagg aaacagttta ctccgcaata
      301 cttgaggttg agaacttcag atttttaatt cttgcattgg ctgttatttc aaatgcattt
      361 gttgtgatgg ttacaattct tgttggacta aaaccatttt tcggaaatat actgccaact
      421 ccaaaatcgc ctcacgaagc accgctgaaa atgctgatcg gtccaataat tcttgctgct
      481 ttcggattac tttccggatt attcccgaat gttttcatac agaaactttt agatcaatca
      541 actataagta taatttctga agagctcgga attgaagtaa aactttggca tggattcaat
      601 tttgttctgc tattaagctt tgtaactctt gcgttaggaa ttagcatcta ttatttcagg
      661 aaacaaatta tccaagcact agccaatata tctttaacaa atttcattaa gccgtcatac
      721 tggtatgaaa ggattttcaa tgggactcta tcctttggaa aatttcaaac aaaaatttta
      781 cagaacggat atctgagata ttatattatc tttataatac ttaccacttt aggattagca
      841 gaatatgcat tgttccaaat tcctttatga gttcattaaa tattgatact gatataacaa
      901 tttacgaatt aataatagga ataattatta ttgcttcaac acttctggtt atcacctccg
      961 attcaagatt aaaagcaatt gtatctcttg gagtcgttgg ttttgcaatg ggaattattt
     1021 ttattctgta cagcgcccct gatttagccc tgacaacttt cgcaatcgaa acattaacag
     1081 taatcctttt tgtgcttgtt ctttacaaac ttccaaagta tcaaaaattc tcaaaaccag
     1141 gaagcaaaat aagagatgca attattgctg gcagtgttgg tgtttttatg atgcttgtcg
     1201 ttctgcttgt taccgctgga gaaatgcagt ctgaattaaa aaaatatttt gcagaggcta
     1261 gtttaccgga gggtaaaggc agaaacattg taaatgttat tctcgttgat ttccgtgcat
     1321 tagatacgct cggagaaata accgtacttg cagttgctgc aataggagtt tatgcactgc
     1381 taaagctgcg attgaaggaa ggagaaaaat aatgtactca gtaattcttt caacagcttc
     1441 aagatatctg tttccgttaa tgctgatatt ttcttttttc ttatttctgc gtggtcataa
     1501 tgaaccaggc ggtggtttcg tcggaggact tgtagccgcg gctgcttatg cactttactt
     1561 aatagcaaat ggtgttgaag aagcaaagaa attacttaaa gctgaaccaa ttaaattaat
     1621 tgcgatagga ttacttcttg ctattatcag tgcattcata tctgtgatta ttggtcagga
     1681 tttcatgact ggcttgtggg ctaaaaatga attccctgtt ataggtaaag tagggactcc
     1741 acttttgttt gatctcggcg tttactttct tgtacttgga atagtcacta aaattatatt
     1801 ctcccttgca gaggaggata aacaatgacg ttccttctcg caatattagt tggcgttctt
     1861 tactcagctg ggatttatat gatcctgcgc agaagtctgg ttaaagtaat tattggatta
     1921 atctttttag gacacgcggc caatcttttg atcttcacga tcggaagagt cacaaaggga
     1981 attccggcat tcgtaccaaa aggttcagaa atacttgctg aaccttttgc tgatccttta
     2041 ccacaggcat taattttaac tgctatagtt ataggatttg gtgtccaggc ttttgcaatt
     2101 gttcttttca aacgtgcata tcagactgtt gaaacagatg atctagataa aatgaaatcg
     2161 actgatgaac tgaaagagga taaacaatga atttgataat tgtattgccg attattattc
     2221 ctcttttgtt tgcagttata atgctcttct tctggaagaa cgtaaatgct cataaaattt
     2281 taaatgttat tgcatcatta ttctctcttg cggtttcaat aattcttctt tcagatgtaa
     2341 atgcttcagg gataatttct ttgcaagttg gaagctggca ggcaccattc ggtatttcac
     2401 tcgttgctga tcttcttgca tcaataatgg ttttgatcac tgcagtaatc ggattagccg
     2461 catcaattta ttcactcggc ttgatggatg agcagcggga aaagtttctt tattatccat
     2521 tacttcaatt tcttttaatg ggaattaatg gtgctttctt aactggagat attttcaatc
     2581 tctatgtttg gtttgaagtg atgctgattt cctcttttgt gcttttagca ttgggaggaa
     2641 aacgtgaaca acttgaaggc gcaatcaaat atgtaacatt aaatcttctt tcatcggcaa
     2701 tatttcttgc tgctgttgga atactttacg gaattgtcgg cactctcaac atggcagatc
     2761 tcgcaagaaa aatttcttta gttcaacaga aagaattagt tactgttgta tcgattttat
     2821 ttatgattgc ttttggaatt aaagctgcag tcttcccgct tttcttttgg ttaccggcat
     2881 catatcacac tccgccaatt gcagtctcgg caatctttgc aggccttctt actaaagtcg
     2941 gcgtctatgc cttaataaga atattcacat taatatttgt t
//
LOCUS       DASWBY010000664         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_102195, whole genome shotgun sequence.
ACCESSION   DASWBY010000664 DASWBY010000000
VERSION     DASWBY010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102195"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VF870_12110"
     CDS             complement(<1..309)
                     /locus_tag="VF870_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252981.1"
                     /translation="MYRRFYFLVLAVLVLFNISYSQSFLTHNTGVLQASVFGNGYIGH
                     NFDGSVGNGVKFGAAPDAMFTAGLFFGNLNFGVNGMVGSFVSGTPQVPIIADMQNTSP
                     L"
     gene            complement(508..1020)
                     /locus_tag="VF870_12115"
     CDS             complement(508..1020)
                     /locus_tag="VF870_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252982.1"
                     /translation="MVLNWTTETEINNQGFEVQRRSAEGEFVTIGNVQGNGTTTERKQ
                     YSYSDAGVETGSYYYRLKQIDFGGAYEYSNEIFVDVTAPLEFALDQNYPNPFNPTTTI
                     NFSLAEPTFVKLAIYNLLGEEVQVLKNEFMNAGSFNVTFDAASLPSGMYLYKVETAQF
                     SSVRKMMLMK"
     gene            complement(1154..>1673)
                     /locus_tag="VF870_12120"
     CDS             complement(1154..>1673)
                     /locus_tag="VF870_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HEX9252983.1"
                     /translation="MFEAISGAVLIMFMRICDVSIGTMRTILVVQGRKYLAGLAGSIE
                     VLIWVFAIRYIFQNLDQVINLFGYSIGFGLGNIIGITIEQKIGLGFAQLNIISKIASE
                     KIIEILRKQKYGVTVLPAHGVSGQLSIIVVIIPRKFQKKLIKLIESIDSNAFITVQHS
                     LPYRGFIHGSRK"
BASE COUNT          603 a          289 c          231 g          550 t
ORIGIN      
        1 taatggagag gtgttttgca tatcagctat aattggcact tgtggagttc cggacacaaa
       61 acttccaacc ataccgttta ctccaaaatt aagatttcca aaaaataatc cagcagtaaa
      121 cattgcatct ggtgctgcgc caaattttac accgttacca acagatccat caaaattatg
      181 cccgatgtat ccgtttccaa atacactggc ttgcaaaact ccggtgttat gcgttaagaa
      241 agattgcgaa taacttatat tgaataatac aagtacagct aacactaaaa agtaaaatct
      301 ccgatacata ctagactcca ttaaatagtg aaataataaa ttaattaaag taccagatta
      361 tcttgtccaa ataatacaaa aatttttgaa aataaatcaa gaaaagattt taaaaagatt
      421 ttcagaaaaa ttggtttgaa taggaattct caaaacaaaa agagccgcat aaagcggctc
      481 aaattaaatc aaatgtgttt aatgaaatta tttcatcaac atcatttttc taacacttga
      541 aaattgagca gtttcaactt tgtacagata cattccactt ggtagtgaag ctgcatcaaa
      601 tgtaacatta aatgatcctg cattcataaa ttcattttta agaacttgaa cttcttcgcc
      661 caataagttg taaatagcta atttaacaaa tgtaggctct gctaatgaga aattaattgt
      721 tgttgttggg ttaaatggat ttggataatt ctgatcaaga gcgaattcta aaggcgcggt
      781 aacatcaaca aatatttcat tgctgtattc ataagctccg ccaaaatcaa tttgctttag
      841 tctgtaataa taacttccgg tttcaacacc ggcatcagaa taagaatatt gttttctttc
      901 agtagtagtt ccatttccct gaacattacc aattgtaaca aattctcctt cagcgcttct
      961 tctctgaact tcaaatcctt gattattaat ttctgtttcc gttgtccagt ttagaactat
     1021 agttttataa agttatatga ttaataaata cacttaatag ctttaatgaa ttgcatatag
     1081 taattcctta aaacaaataa ttaaatgtaa tgtaattaag tgagttgaaa tattttcatt
     1141 aaaaaaaatg aaactatttt cttgaaccat gaataaatcc acggtatggt aatgaatgct
     1201 gaacagtaat aaatgcattt gaatctattg actcaattag tttaattaat ttcttctgaa
     1261 attttcttgg gataataaca acaattatcg ataactgtcc cgaaactccg tgtgcaggta
     1321 gaacagttac accatacttt tgttttcgta atatttcaat aatcttttca ctagctattt
     1381 ttgagattat attaagctga gcaaaaccta atccaatttt ttgttcgatt gtgataccaa
     1441 taatgtttcc aagaccaaaa ccgattgaat atccaaacag atttattact tgatcaagat
     1501 tctggaaaat gtatctaatg gcaaaaaccc agataagtac ttcaatagaa ccagctaacc
     1561 ctgcaagata ttttcttccc tgtacaacta atattgttct catcgtaccg attgatacgt
     1621 cacaaatacg cataaacatt attaataccg cacctgaaat tgcttcaaac atg
//
LOCUS       DASWBY010000665         1997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_441448, whole genome shotgun sequence.
ACCESSION   DASWBY010000665 DASWBY010000000
VERSION     DASWBY010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441448"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF870_12125"
     CDS             complement(<1..203)
                     /locus_tag="VF870_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX9252984.1"
                     /translation="MKNISKLDSASAIAIRDCMGAKKNEKILVITDEQKREIGLSLHE
                     NAIRLGYESLLVEMKSGKINGEEP"
     gene            complement(218..973)
                     /locus_tag="VF870_12130"
     CDS             complement(218..973)
                     /locus_tag="VF870_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252985.1"
                     /translation="MSKIFKKIFFLFFIFSLQILPQSLGFGCFGFVGGFGGYSYQQYQ
                     PTGLNIFVANFNSINSEYIENELPEFNTASGYRVGINFFRAKYTGFFITAKGFYQQLG
                     ESNSAEVIPTSVTINYDFDLKLNSWGIGVDLGIPITNYLSWKILDGSLLINSARFTET
                     INSSQGTQVNKYNNDKTELGYSIGTGFILEVLEDYISLEGVASYSQITIDKMKAEDGT
                     NLIYNPDKNINTEKFIKYGGFNAVVQLNIGFPL"
     gene            complement(1029..>1997)
                     /locus_tag="VF870_12135"
     CDS             complement(1029..>1997)
                     /locus_tag="VF870_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9252986.1"
                     /translation="ITVAGGVFAFICVVMYFFLDIYASKNYELPKEGEQDAILLSDIL
                     RINEKTRWYIFSILAAAVIILSFVNNDNWAIYLISLIVITLVLFLKPKSFSNSFWYIS
                     ALCVTFYSAMFPFQTFAIKFFQEAHGTSREVGGNLSSILTLAAMIFTPLFGLLADKIG
                     KRSLLMMFGSLLIIPVYLMMAYKFGKPDVMGEGDFINLTFSFFDINAAIPIYLILPMA
                     MMGIAFSLVPAVMWPSVALIVDSNKLGTAYGLMTMIQNIGLFGFNLLIGFTNDYAGAS
                     ASNPGGYTPGMWIFSILGFLGLTFAFLLRKSETGPNAHGLEKGMAK"
BASE COUNT          688 a          383 c          281 g          645 t
ORIGIN      
        1 ggttcttcgc cgtttatttt tcctgatttc atttctacaa gtaaagattc atatcctaat
       61 cttattgcat tctcgtgtaa tgataatcca atttcccgtt tctgttcgtc tgttataaca
      121 agaatttttt cattcttctt agcacccata caatcacgaa tcgcaatagc tgatgctgaa
      181 tcgagtttac ttatattttt cattttactc ctctgtacta aagtggaaaa ccaatattta
      241 attgaacaac tgcattgaag ccgccatact taataaactt ttctgtattt atatttttat
      301 ccggattgta aattaaattt gtaccatcct cggccttcat tttatcaatt gttatttgag
      361 agtaacttgc aacaccttcc aaacttatat aatcttcaag tacctctaaa ataaagcctg
      421 tcccgattga atacccgagt tctgttttat cattattgta tttgtttact tgtgtaccct
      481 gcgatgagtt aattgtttct gtgaatctgg cactattgat tagtaagctt ccatctaaaa
      541 ttttccagct taaataattt gttatcggaa ttccaagatc gacaccaata ccccagctgt
      601 ttaatttcaa atcaaaatca taattaatgg tcactgaggt gggaataact tcagcactgt
      661 tactttcacc tagctgttgg taaaaaccct tggcagtaat aaaaaatccg gtgtactttg
      721 ctcgaaaaaa atttatccct accctgtaac ctgaggctgt attaaattca ggcaactcat
      781 tttctatata ctcagaattt attgaattaa aatttgcaac aaatatattt aatccagtcg
      841 gttgatattg ttggtagcta tatccgccaa agccacccac aaacccgaag caaccaaacc
      901 ccaatgattg cggaagaatc tgtaaactaa agataaaaaa cagaaaaaat atttttttaa
      961 atattttact cataaaattt taattcagta ttcactaaaa agtacactca ttaattcaaa
     1021 tgagcatttt attttgccat tcctttttca agtccatgtg catttggtcc ggtttctgat
     1081 tttctgagca agaatgcaaa tgttaatcct aagaacccga gtattgaaaa tatccacatt
     1141 ccgggagtat aaccaccggg atttgaagca cttgctccag cataatcatt tgtaaaacct
     1201 attaataaat taaagccaaa taatccaata ttttgaatca ttgtcattaa accgtaagct
     1261 gtacctaatt tgtttgaatc aacaatcaaa gcaactgaag gccacataac agcaggcaca
     1321 agtgagaatg caattcccat catcgccatc ggcagaatta aataaattgg aatagccgca
     1381 ttaatatcaa agaatgaaaa agtaagatta ataaaatctc cttcacccat cacatcgggt
     1441 ttaccaaatt tataagccat cattaaatag acaggaatga ttaacaaaga tccaaacatc
     1501 atgagcaatg atcttttgcc gatcttatct gcaagtaaac caaacaatgg ggtaaagatc
     1561 attgcggcaa gtgttagaat acttgaaaga tttcctccaa cttctctgct ggttccatgc
     1621 gcttcctgaa aaaatttaat tgcaaatgtc tgaaacggaa acatcgctga atagaaagta
     1681 acgcataaag cagaaatgta ccagaatgaa tttgaaaaag atttaggttt taagaaaaga
     1741 acgagcgtaa taacaattaa actgataaga tatatcgccc agttatcgtt attaacaaaa
     1801 cttaatataa ttactgccgc agctagtatt gaaaatatat accatcgtgt tttttcattt
     1861 attcttaaaa tatcagaaag taaaattgcg tcctgttctc cttcttttgg cagttcataa
     1921 ttcttgcttg cataaatatc taaaaagaaa tacataacaa cgcaaataaa tgcaaacact
     1981 ccacccgcaa cagtaat
//
LOCUS       DASWBY010000666         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_254142, whole genome shotgun sequence.
ACCESSION   DASWBY010000666 DASWBY010000000
VERSION     DASWBY010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254142"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VF870_12140"
     CDS             <1..239
                     /locus_tag="VF870_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252987.1"
                     /translation="PNPFNPTTSIRFNLPEAGNVKLAVYNLLGQEVKTLVNGFRTAGA
                     YTINFDASNLSSGIYLYKIEMNNFTQTRKMTLLK"
     gene            339..1262
                     /locus_tag="VF870_12145"
     CDS             339..1262
                     /locus_tag="VF870_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX9252988.1"
                     /translation="MSKRIIITGATGLIGKKLADALIARGDEVIIFSRDAKRAKSIFP
                     NAIECVEWNYKRPKQWKSRLEYVDAVIHLAGINLFAKRWNDNFKKAVLESREVSTKNL
                     VEAIKLAANKPEVFVCASGIGYYGDRADDLLTEESPAGNDFLAEVCKVWESEASKVSG
                     FGIRNVQVRTGLVLSLEDGALKQMLPPFKYFIGGPLGNGKQWSSWLHIDDIVRIYLHA
                     IDNKNLSGAVNAASPNPVRMKEFANTLGKALHRPSIFSVPKFVLKIVVGEAAEVVTAS
                     QKVDDQKLLNSRYKFKFPDLYKALQNLLSQN"
     gene            1373..>2301
                     /locus_tag="VF870_12150"
     CDS             1373..>2301
                     /locus_tag="VF870_12150"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9252989.1"
                     /translation="MLGTSTLNQVLRSLRHRNYRLFFGGQGISLIGTWMQQIALGWLV
                     YRLTDSAFLLGLVGFAGQIPTFILASFAGVLADRYDKHKIIIITQTLAMIQALVLAIL
                     TLTHTIQIWHIIFLSVVVGIVNAFDMPTRQSFVIDLVDDKSDLPNAIALNSSMFNTAR
                     LVGPTVAGILIAVLGEGLCFLLNAISYIAVIIALFMMNITPKNENRKQAKVLKGLKEG
                     IKYAYSFKPIRILLIFLGLISLTGGPYTVLMPIFAKDILKGNANTLGFLFGAVGIGAL
                     IGAVYLASRKTVLGLGRWIAISSSVFAMGLILFS"
BASE COUNT          731 a          350 c          446 g          774 t
ORIGIN      
        1 atccaaatcc ttttaatcca accacttcaa tcaggtttaa tttgccagaa gctggtaatg
       61 taaaacttgc tgtttataat ttgcttggac aggaagttaa aactttggtt aatggattta
      121 gaactgctgg tgcttataca attaactttg atgcaagtaa tttatcaagt ggaatttatc
      181 tctacaaaat tgagatgaat aatttcactc aaacaagaaa gatgactctg cttaaataat
      241 taaagattta atttgtcttt aaagccccga atttattcgg ggctttttta ttttgaacga
      301 ataatctttt tatttgttta gatctttaaa ggattaatat gtcaaaaaga attataataa
      361 ccggagctac cggcttaatt gggaaaaaac ttgcagatgc cttgatcgcc agaggtgatg
      421 aagtaataat tttcagcaga gatgctaaaa gagcaaaatc cattttccca aacgctattg
      481 aatgtgttga atggaattat aaaagaccta aacaatggaa atccaggtta gaatatgttg
      541 atgcagtaat tcatcttgct ggtataaatc tatttgccaa aagatggaat gataatttta
      601 agaaagcagt tttagaatca cgggaagtaa gtacaaaaaa tttagttgag gcaattaaat
      661 tagctgccaa taaacctgaa gtttttgtgt gtgcctccgg aattggttat tatggagatc
      721 gcgctgatga tttattaaca gaagaatctc cagctggtaa tgattttctt gccgaagtgt
      781 gtaaagtatg ggaaagcgaa gcaagtaagg ttagtggatt tggaatcaga aatgttcaag
      841 ttagaacagg attagtttta agtttagaag atggtgcttt gaaacaaatg ctgcctccat
      901 ttaaatattt tatcggtggg cctctgggaa atggaaaaca atggtcatcc tggctgcata
      961 ttgatgatat tgttaggatt tatttacacg ctatcgataa caaaaattta tccggagccg
     1021 taaacgcagc ttcaccaaat ccagtaagga tgaaggagtt tgcaaacacg ttaggtaaag
     1081 cattgcatcg cccatcaatt ttttccgttc caaaatttgt tttaaagatt gtcgtgggag
     1141 aagcagcaga ggttgtaact gcaagccaaa aagttgatga ccaaaaactt ctcaacagtc
     1201 gatataaatt taaatttcct gatttatata aggcattaca aaatctttta agccagaatt
     1261 aattattgag attttttatc acgttaaaaa tattttatcc tgcttgtgct ctagattatt
     1321 tcatcattcc agcgtattcg ttaatttatt ttaaagattg aaataattat ttatgcttgg
     1381 cacatcaact cttaatcagg ttttaaggtc attacgacat agaaattatc gtttgttttt
     1441 tggcggacaa ggaatttctt taataggcac ctggatgcag caaatcgcct tgggatggtt
     1501 ggtatacagg ttaactgatt cagcttttct tttaggtttg gttggttttg caggtcaaat
     1561 ccccacattt attttagcat cgtttgcagg agttttggca gatcgatacg acaaacacaa
     1621 aataattatt atcactcaaa ccttagcaat gatccaggcc ttagtacttg caattctaac
     1681 tttaacccat accattcaga tctggcatat aatttttctt agtgtagtag ttggaatagt
     1741 aaatgcattt gatatgccaa ccagacaatc tttcgtgatc gatcttgttg atgataaatc
     1801 tgatttgcca aacgcaatcg cgctgaattc atctatgttt aatactgcaa gattagtagg
     1861 accaacagtt gcagggattt taattgcagt gttgggagag ggtctttgtt ttttacttaa
     1921 tgctattagc tacatagcag taattattgc tctttttatg atgaatataa ctcctaaaaa
     1981 tgaaaaccgg aaacaagcaa aggtgttaaa aggattaaaa gaaggaataa aatatgctta
     2041 tagttttaaa cctatcagaa ttcttttgat atttcttgga cttataagtt taacgggcgg
     2101 gccttacacc gtgttaatgc caatcttcgc caaagatata ttaaagggga atgctaatac
     2161 gctgggattc ctttttggtg cggttggcat cggggctttg attggtgctg tctatcttgc
     2221 atcacgcaaa acagttttag gtcttggcag atggatagca atttcttcat cagtttttgc
     2281 tatgggatta atactctttt c
//
LOCUS       DASWBY010000667         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_254151, whole genome shotgun sequence.
ACCESSION   DASWBY010000667 DASWBY010000000
VERSION     DASWBY010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254151"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VF870_12155"
     CDS             <1..238
                     /locus_tag="VF870_12155"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252990.1"
                     /translation="NPFNPSTKISWYSPIGSWQTLKIFDALGREIETLVNEYKDAGVH
                     STFFNVSSTLPSGIYFYQLKTENYIETKKMILLK"
     gene            complement(241..>1628)
                     /locus_tag="VF870_12160"
     CDS             complement(241..>1628)
                     /locus_tag="VF870_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9252991.1"
                     /translation="AGNNWFGSDTCKGANIFFHGGDPGIAIDKNGTFILTRKGTTTFQ
                     GIYSHYSTDMGITWSAQNTISTEEIERATIISDNDPNSAYYGRTYACWAKLVPNYTIG
                     FSYTNDGGQNWVTSTQLSNTILRRAGAEIDVGQDNQVYICWAGIKSVSPFTEELVGFA
                     SSTNGGANWTVNENVFPMSGIVGILPEKQSIRVNGLPRMAVDVSNTSTRGTIYIVTSQ
                     KNLLPAGSDPDIILRKSTDGGQSWSSAIRVNQDAQNNGKSQFFPGITIDEYGGVNIIY
                     YDDRNTTIDSLGVYLSRSTDAGNSWTEYPISNHNFKPIAIGGLGQGYMGDNIDITSVG
                     NKLYPVWMDNSTGIYQIWSVPIEILQVGLENENNIVLPLGYELKQNYPNPFNPNTAIE
                     FYLPISSNVSLKIFDTTGREVGTLLNDYRESGNHTINFDANKFNLSSGVYFYMLSANG
                     FKQTKSMVLLK"
BASE COUNT          512 a          316 c          263 g          537 t
ORIGIN      
        1 aaatccattc aatccaagca caaaaattag ttggtactct ccaattggca gctggcaaac
       61 tctaaaaata tttgatgcac taggcaggga gatagaaacg cttgtaaatg agtacaaaga
      121 tgctggagtt cattctacat tttttaatgt aagttctaca ttgccaagtg gaatttattt
      181 ttaccagtta aaaacagaaa attatattga gactaaaaaa atgatactgc taaaataaaa
      241 ttattttagc agcaccattg attttgtttg cttaaatcca ttagcagaaa gcatataaaa
      301 atatacaccg ctcgataaat taaatttatt agcatcaaaa tttattgtgt gattccctga
      361 ttctctgtag tcgttaagaa gcgttccaac ttctctccct gtagtatcaa aaattttcaa
      421 cgatacattt gaactgatcg gtaaataaaa ttcaatagcc gtatttggat taaatggatt
      481 tgggtaattt tgttttaact cgtaacctaa tggtaaaaca atattatttt cattttctaa
      541 accaacttga agaatttcaa ttgggacact ccatatttga taaattccgg ttgaattatc
      601 catccaaacc ggataaagtt tatttccaac cgaagtgata tcaatattat cacccatata
      661 tccttgtccc aatccaccga ttgcaattgg tttaaagtta tgattgctta taggatactc
      721 agtccagcta tttcccgcat cagttgatct tgaaagatag actcctaaag aatcgatagt
      781 tgtatttcta tcatcataat aaataatatt aactccgcca tattcatcaa tagtgattcc
      841 ggggaaaaac tgacttttgc cgttgttttg agcatcctgg ttaacacgaa ttgctgaaga
      901 ccaggattgc cctccatcgg tagactttct taaaataatg tcagggtctg atcctgcagg
      961 taatagattt ttttgagacg ttacaatata aattgtccct cgcgtagatg tgtttgaaac
     1021 atcgactgcc attcttggta aaccattaac tctaatactt tgcttttctg gaagtatccc
     1081 tacaattcca ctcataggaa aaacattttc gtttactgtc cagtttgcgc cgccatttgt
     1141 agaagatgca aaaccaacaa gttcttcagt aaagggcgaa acacttttta tccccgccca
     1201 gcaaatgtaa acctggttat cttggccaac atcaatctct gcgcccgctc gtcttaagat
     1261 tgtgttgctc aattgagttg aagttaccca attttggcca ccgtcatttg tgtaactaaa
     1321 tccaatagtg taattaggaa caagttttgc ccaacacgca tatgttcttc catagtaagc
     1381 actatttggg tcattatccg agataattgt tgcccgttca atctcctctg tcgaaatagt
     1441 attttgtgcg gaccaggtga ttcccatatc agtagaataa tgagaataaa tgccttgaaa
     1501 tgtagtggtc ccttttcttg taagtataaa cgttccattt ttatctattg caattccagg
     1561 atcaccacca tgaaaaaaaa tatttgctcc tttgcaagta tctgatccaa accagttatt
     1621 acctgcgt
//
LOCUS       DASWBY010000668         3658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_102316, whole genome shotgun sequence.
ACCESSION   DASWBY010000668 DASWBY010000000
VERSION     DASWBY010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102316"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VF870_12165"
     CDS             complement(<1..922)
                     /locus_tag="VF870_12165"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9252992.1"
                     /translation="MNSNSVLQIKNVLLLIILISIQLFPQRDSSTLDQYNFLTDQIIR
                     KTLTEKPGYKLLQELCDVGPRLSGSENSLRAIHWAEEKLKSFEVDRVWLQPVMVPHWE
                     RGSVETAEIINGKSLNILSLGGSISTPENGITANVIEVKNFTELKERQNEVKGKIVFF
                     SRPLDEGLLNTFAGYGGAVDQRFYGAVEAAKYGAVAVLIRSITTKHDNVPHTGVMGYA
                     DSLQKIPAAAIGYLDSDYLHNALVENPDLKIKLNMDCKTFPDAQSYNVIAEIKGTEFP
                     DEIIVVGSHFDSWDVGCGAHDDGAGCVQSME"
     gene            complement(1058..>3658)
                     /locus_tag="VF870_12170"
     CDS             complement(1058..>3658)
                     /locus_tag="VF870_12170"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEX9252993.1"
                     /translation="DVSFGRFPNGTNNWFQFSPATPGAANLESGIFNILSAPTISLQS
                     GFYSSPINISASYSLPDAVIRFTSDGTIPTTSSNVYSSPLQIDSTSVLRFRAFKDGYT
                     PSIAETRTYFINETTDLPVFSLVTDPANFFSDTSGIYVAGTNGIIANCSTQPRNWNQD
                     WERPVSLEFFEFDKSLAFKVSTGVKINGGCSRLYPEKSLAFYFRGEYGDDKLRYRLFD
                     DIPVYEYNNFILRSSAQDWWRTMFRDGMVQTLIEQGMKIDYQNYRPSILFINGEYWGI
                     HNIREKLNEHYVYYHHGVNKDNIDLIEISKGIVANSGDVAAYNEMINFLSTNNMANPA
                     NYEYIKSIVDVDEYIDYQIAEIYAANGDWPGSNMKLWRERVAGSKWRWMIYDLDFTFG
                     GNAQGLATTNTLVQATATNGPDWPNPPWSTLMLRMMLNNPDFKNEFIQRMAAHINTTF
                     EPNHVLAVIDSMAQNIASEIPRHKERWPQSISFGDSWQELIDIMRNFAIDRPLNIREH
                     FYSKFGISGSSSLVISRNNPAWGKIFTNTVEVKNNGSTNKFFKDIPVHIRALPMPGYK
                     FVRWEGISTETSPEISIVLNGNSILTAVFDPAELTVTSIVINEINYKSSSVFDTEDWV
                     EFYNPDNVSVDISGWKFSDGDIANQFTFTSGTILGANDYLVLCRDTVKFKLLHPNQNK
                     ILGNISFGLSSDGDHLILKDNSENLIDEVAYSSSGLWTSLPNGSGPTLSLINPQLDNS
                     LAESWQASGLYGTPGYLNDVYTKVELENSLVPSEFILYQNYPNPFNPVTTIRYSIPNV
                     TLSGVEGARVQLKIYDVLGNEVATLVNEYRIAGSYEVEFNASHLSSGIYFYKLQASTF
                     VETKKMILLR"
BASE COUNT         1148 a          713 c          586 g         1211 t
ORIGIN      
        1 cttccattga ttgaacgcag ccggccccat catcatgcgc accgcatcca acatcccagc
       61 tatcgaaatg tgatccaacg acaattattt cgtctggaaa ttctgtgcct tttatttctg
      121 caattacatt gtatgattga gcatccggaa aagttttaca atccatattt aatttgattt
      181 ttaaatcggg attttcaacc aaagcattat gcagataatc agaatccaag taacctattg
      241 cggcagcagg aattttttgc aatgaatctg cataacccat tacacctgtg tgaggtacat
      301 tgtcatgttt cgttgtaatg gatcgaataa gaacagcgac agctccgtac tttgctgctt
      361 ctacagcacc ataaaacctt tgatcaacgg cacctccata tccggcaaag gtattaagca
      421 acccttcatc aagagggcga ctaaagaaaa ctatttttcc ctttacttcg ttttgccttt
      481 cttttaattc agtaaagttt tttacttcaa ttacatttgc tgtgatccca ttttcaggag
      541 ttgagatgct tcctccaaga gaaagaatat ttaaagattt tccattaata atttcagctg
      601 tttcaacact tcctctttcc caatgaggaa ccataacagg ctgcagccaa actctatcaa
      661 cctcaaagct tttcaatttt tcttcagccc aatgaatcgc ccttaaagaa ttttctgagc
      721 cgcttaatct aggtccaaca tcgcaaagtt cctgtaataa tttgtatccg ggtttttcag
      781 tcaaagtttt tcttattatt tgatcagtta aaaaattgta ttgatctaac gttgaagaat
      841 cccgttgtgg aaataattga attgatatta aaatgattag aagtaaaaca tttttaattt
      901 gtaaaacaga attggaattc aaataatctc tcctgtaaat aaaaatttat aatcaatttc
      961 ttctttaagg aaaaaatagc tttgatgttt attaaaacaa agatgtgttt taagaatttt
     1021 tagttaagaa aatattccca caaaagaaat cttaagctta ccgtaataaa atcatctttt
     1081 tagtttcaac aaatgtgctt gcctgcagtt tgtaaaaata aattcctgaa gataaatgag
     1141 aagcattaaa ctcaacttca taacttcctg caattctata ttcattaacc aaagttgcaa
     1201 cttcatttcc aagtacatca taaattttaa gctgcactcg agccccttcg actccgctca
     1261 gggtgacatt tgggattgaa tatctaattg ttgtaactgg attaaatgga ttggggtaat
     1321 tctgatataa aataaattcc gatggaacta aactattttc cagctcaact tttgtatata
     1381 catcatttaa atatccgggg gttccataaa gaccggaggc ttgccagctt tctgcaagcg
     1441 agttatctaa ttgaggatta atcaatgata atgttggtcc acttccattt ggtaatgatg
     1501 tccagagccc gcttgatgaa tatgcaactt catcaataag attctctgaa ttatctttca
     1561 gaattaaatg atcaccatcg ctgcttaagc caaaacttat gtttcctaaa attttgttct
     1621 ggtttggatg aagtagttta aatttaactg tatctctgca tagaactaaa taatcatttg
     1681 ctccaagaat tgttccgctt gtgaatgtaa actgatttgc aatatctccg tcggaaaatt
     1741 tccatccgga aatatcaaca gaaacattat ccggattgta aaattcaacc caatcttcgg
     1801 tatcaaaaac tgaagaagat ttataattta tttcgtttat tacaatagac gtaaccgtta
     1861 actccgctgg gtcaaatact gctgttaata ttgagttacc attcagcaca atagatattt
     1921 caggagaggt ctcggtagat atgccctccc atcttacaaa tttatatccg ggcattggta
     1981 atgctcttat gtgaacagga atatctttaa agaatttatt cgttgatcca ttatttttta
     2041 cttccacggt gtttgtaaat atctttcccc aggccggatt gtttctgctg atcaccaaag
     2101 aacttgatcc gctaatgcca aactttgaat aaaaatgttc tctaatattt agaggtctat
     2161 ctatagcaaa gtttctcata atatctatta gttcctgcca gctgtcacca aaagaaattg
     2221 attgcggcca gcgttctttg tgtcttggaa tttctgaagc aatattctga gccatactat
     2281 ctattactgc taaaacatga ttcggctcga acgttgtatt tatatgtgca gccattctct
     2341 gaataaattc gtttttaaaa tccggattat tcaacatcat tcttaacatt aatgttgacc
     2401 atggggggtt cggccaatca ggaccattag ttgcggttgc ctgtacaaga gtatttgttg
     2461 ttgccaggcc ctgtgcgttt cctccaaaag taaaatcaag atcataaatc atccatcgcc
     2521 attttgaacc ggcaacacgc tcacgccaaa gcttcatatt tgatccgggc caatcaccat
     2581 tagccgcgta aatttctgcg atctggtaat caatgtattc atcaacatct acgattgatt
     2641 taatatattc ataattagcc ggatttgcca tattgtttgt agaaagaaaa ttaatcatct
     2701 cattataagc agcaacatcg ccgctgtttg ccacaatgcc tttacttatt tcaatcagat
     2761 ctatgttatc tttattaact ccgtgatgat aataaacata atgctcatta agtttttctc
     2821 ttatgttgtg tatgccccag tactcgccat taataaataa tattgatggc ctatagttct
     2881 ggtaatctat tttcattcct tgttctataa gcgtttgaac cattccatca cgaaacattg
     2941 ttctccacca atcctgtgcg gagcttcgaa gaataaaatt gttatactcg taaaccggaa
     3001 tatcatcaaa caaacgataa cgtaatttat catctccgta ctcacctcta aaataaaatg
     3061 ccagagattt ttccggatat aaacgactgc atccgccatt aattttaact ccggtactaa
     3121 ctttaaatgc aagtgattta tcaaactcaa aaaactccaa actaaccggt ctttcccaat
     3181 cctgattcca gtttcttggt tgagtactgc aattagcaat tattccgttt gtgcccgcaa
     3241 cataaattcc agaggtatca ctaaaaaagt ttgccggatc agtaacaaga gaaaacactg
     3301 gaagatccgt tgtttcatta ataaaataag ttcttgtttc ggcaatgctg ggcgtgtaac
     3361 catctttaaa tgcacggaac cttaaaacag aagttgaatc aatttgtaaa ggacttgaat
     3421 atacattgct tgatgttgtt ggtattgtgc catcgcttgt aaatcgtata actgcatccg
     3481 gtaagctgta agaagcagaa atatttatag gcgatgaata aaatccactt tgtaaagaaa
     3541 ttgttggagc tgaaagaata ttaaaaattc cagattctaa atttgcggcg cctggtgttg
     3601 ccggagaaaa ttgaaaccaa ttatttgttc cattaggaaa tcttccaaaa gaaacatc
//
LOCUS       DASWBY010000669         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_63308, whole genome shotgun sequence.
ACCESSION   DASWBY010000669 DASWBY010000000
VERSION     DASWBY010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63308"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VF870_12175"
     CDS             complement(<1..674)
                     /locus_tag="VF870_12175"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9252994.1"
                     /translation="MFLSVSSLAQTGVNNKIANDKKYFVSDLQKQIDVLHYNLKFDLN
                     SNDKVLKGVSTITAVKNNPNSDSEVEHEADIELNLYDNMSITSLKVNGKESEYFRNKN
                     RVFIKANEILSDTFKIEIVYNGTPKRSGFDGFVFGEVNGQSLVYNISEPIYASTWFPC
                     DDDPADKALLDIEITNYSQFVSVSNGNLVDVETENDKKTYHYKTEYPISTYLVAVYSA
                     PYKTFY"
     gene            834..2075
                     /gene="glgC"
                     /locus_tag="VF870_12180"
     CDS             834..2075
                     /gene="glgC"
                     /locus_tag="VF870_12180"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX9252995.1"
                     /translation="MNSPGSSILRDTVTVILAGGQGERLHPLTAVRSKPAVPFGGKYR
                     IIDFALSNCLNSGFRRVYVLTQYKSDSLNMHLYEAWSIFNPELGEFIYSVPPQRKLNN
                     EWYLGTANAIHQNLNLFSDKKAKWVLILGGDHIYKMDYLKFLDNHISQKADLSIASLE
                     VPKDEASRFGIVGVDDDYNILSFIEKPANPPQIPDKPGYSFVNMGIYVFNANVLREVL
                     QEMESKKIKNHDFGQHVIPYMVQSKLKVIAYKFQDENKKTQPYWKDIGTLDSYYAANM
                     DLISVIPDFNLYDPDWPLRTYQYQYPPAKTVSHEGERVGRTLNSLVCDGTIVSGGLVE
                     RSLLGSNVKVNSYSYITDSIIMNNVNIGRHARVRRAIIDKNVIIPEGYEIGFDPEGDK
                     KKFTVTDTGIVVIAKNSVLPD"
     gene            2170..>2777
                     /locus_tag="VF870_12185"
     CDS             2170..>2777
                     /locus_tag="VF870_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9252996.1"
                     /translation="MNTKLKTNLKNLPASPGVYQFINKNGKVIYVGKAKNLKNRVRSY
                     FQENPGSAKTVAMVSKIDDLQLVVTDTELESLILENNLIKQLKPRYNVNLKDDKSFPF
                     IKVTNELFPRIYPTRKVYNDGSKYFGPYTDVRSMRSSLRMINQIFKIRSCKLDLTEKN
                     IADKKFKVCLDYHIKKCDGPCENLISAKAYNEMVDEVIKLLKG"
BASE COUNT          968 a          448 c          473 g          888 t
ORIGIN      
        1 tcataaaaag ttttgtatgg ggcagaatat acggcaacaa gataagttga gattggatat
       61 tctgttttgt agtgatatgt ttttttatca ttctcagttt caacatcaac aagattccca
      121 ttcgatactg aaacaaactg cgaatagtta gtaatctcaa tatcaagtaa agctttatca
      181 gcaggatcat catcgcatgg aaaccaggtt gaagcgtaga ttggttcgct aatattgtaa
      241 actaaagatt gcccgttcac ctccccaaaa acaaatccat caaatccact tcttttcgga
      301 gttccattgt aaacgatctc aattttaaaa gtatcactta aaatttcatt ggctttaata
      361 aatactctgt tcttatttct gaagtattct gattctttac cattaacttt taaactcgtt
      421 atactcatgt tatcatatag attaagttca atatcagctt catgctcaac ttcggaatcg
      481 gaatttggat tattcttaac agcagttatt gtagaaactc cttttaatac cttatcatta
      541 gaatttaaat caaacttaag attataatga agtacatcaa tttgcttctg taaatcacta
      601 acaaaatact ttttatcatt tgcgatttta ttattaactc cggtctgcgc aagagaagat
      661 acacttaaaa acatgattag tattgtaatt aaacttttga atttggatat cataaccttg
      721 aaatactttc taactaaaaa ttatttacat tgagtaaaaa taatttaacc atttgttcaa
      781 ttgccaaaca aactataatt caatatattt tagtgcaaat tttaggagac tatatgaact
      841 cgcccggttc gtccatttta agggacacag ttacagtaat tctcgccggc ggccaaggcg
      901 aaagacttca tcctttaacg gctgtaagaa gtaaacctgc agtaccattt ggaggtaaat
      961 acagaataat tgacttcgct ctatccaact gccttaactc tggttttaga agagtttatg
     1021 ttttgaccca gtacaagtcc gactccttaa atatgcacct ttacgaagcc tggagcatct
     1081 ttaatccgga acttggagag ttcatttatt cagttccacc ccaacgtaag ttaaacaacg
     1141 aatggtatct tggaactgca aatgcaattc atcaaaattt aaatttattt tctgacaaaa
     1201 aagctaagtg ggtattaata cttggcggcg atcatatcta caaaatggat tatctaaaat
     1261 ttcttgataa tcatattagt caaaaagctg atctttctat tgcgtcttta gaagttccaa
     1321 aagatgaggc aagtagattt ggcattgttg gagttgatga cgattataat atactttctt
     1381 ttatcgaaaa gcctgctaac ccgccgcaga tacctgataa gccgggatac tcttttgtga
     1441 atatgggaat ctatgttttt aatgcgaatg ttttaagaga agttttacag gaaatggaat
     1501 ctaaaaaaat aaagaaccat gattttgggc agcatgttat tccttatatg gtacaatcaa
     1561 aattaaaagt gattgcttat aaatttcagg atgagaataa aaagactcag ccttactgga
     1621 aagacattgg aacacttgat agttattatg ctgcaaatat ggatttgata agcgtaattc
     1681 ctgatttcaa tctttacgat cctgactggc cattgcgaac ctatcaatat caatatccgc
     1741 ctgcaaaaac ggtatcacac gaaggagaaa gagttggtag aacgctcaac tcattagtct
     1801 gcgacggtac gattgtttcc ggaggtttgg tggaaagatc attgctcggt tctaatgtaa
     1861 aggtaaacag ctactcgtat attactgatt caataatcat gaataacgta aatattggca
     1921 ggcatgcaag ggtacgcaga gcaataattg ataaaaatgt aattatacct gaaggatacg
     1981 aaattggttt tgatccagaa ggagacaaga agaaatttac cgttacagat actggtattg
     2041 ttgttattgc taaaaattct gttttaccag attaaacggt aaatacaaat cttattatca
     2101 aaaatcattt gattggtttg atcaattaag aaattaaatc agttgaatga atttgattat
     2161 ctcataacta tgaacactaa attaaaaact aacttaaaaa acttgccagc ctctccgggc
     2221 gtttaccagt ttattaataa aaatgggaaa gtgatttatg ttggtaaagc taagaattta
     2281 aagaacagag taagaagtta ttttcaggaa aatcccggct cagcaaaaac cgttgctatg
     2341 gttagcaaga ttgatgattt gcagcttgtt gttacagata ctgagcttga gtcattgata
     2401 cttgaaaaca atttaatcaa gcagttaaaa ccaagataca atgtaaatct taaggacgat
     2461 aaatcattcc cattcataaa agtgaccaac gaactattcc caagaattta tcctacgaga
     2521 aaagtttata atgatggttc aaaatatttt gggccttata cagatgtaag aagcatgcgc
     2581 agctctttaa gaatgataaa tcagatattt aaaattcgca gctgcaaact tgatttgact
     2641 gaaaaaaata tcgctgataa aaagtttaaa gtgtgcctgg attatcacat aaaaaaatgt
     2701 gatggacctt gcgagaactt gatatctgca aaagcttaca atgaaatggt tgatgaagtt
     2761 attaagcttc ttaaagg
//
LOCUS       DASWBY010000670         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_254296, whole genome shotgun sequence.
ACCESSION   DASWBY010000670 DASWBY010000000
VERSION     DASWBY010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254296"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VF870_12190"
     CDS             complement(<1..556)
                     /locus_tag="VF870_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEX9252997.1"
                     /translation="MKNNHLENCKSLIKKNKIEFIDLKAIDLTGRLHHITLPVYPNIL
                     EKLLSEGVGFDGSSYGFRKVENSDMIMIPDLATGQLDPFRDAPTLTFYSHIVLTDEKR
                     TPFNQDGRYLAKKTEELLKKTTGADKSWWGPEFEFYIFSKVEYDTRTASSFYRVEHAE
                     EFYKKAYHAANPFDEYDDFRDEACK"
     gene            complement(649..>1502)
                     /gene="eno"
                     /locus_tag="VF870_12195"
     CDS             complement(649..>1502)
                     /gene="eno"
                     /locus_tag="VF870_12195"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9252998.1"
                     /translation="ILNGGKHADNNVDFQEFMVMPIGAPSFKEALRMGAETFHALKSV
                     LAKKGYNTAVGDEGGFAPNLKSNEEAIEVILEAIEKAGYKVGKDIGIALDPAASEFFI
                     KEKNAYHLFKSAPNQLIPIEKMVDYWAGWAKQYPIVSIEDGLAEDDWDGWKLMTEKLG
                     GKIQLVGDDLFVTNTNRLAKGIDLGVANSILIKVNQIGTLTETLDAIELAKINGYTSV
                     ISHRSGETEDTTIADIAVATNAGQIKTGSASRTDRIAKYNQLLRIEDELDTTGIFPGV
                     AALNYNG"
BASE COUNT          442 a          291 c          236 g          533 t
ORIGIN      
        1 gtttacatgc ttcatcacga aaatcatcat actcatcaaa aggatttgca gcgtgatacg
       61 cttttttata aaattcttcc gcatgctcca ctcgataaaa agatgacgct gtgcgtgtat
      121 catattcaac tttggaaaaa atataaaact caaattctgg tccccaccag gatttatccg
      181 cacctgtagt tttctttaaa agctcttcgg tttttttagc aagatatctt ccatcctgat
      241 taaacggagt tcttttttca tctgttaaaa ctatgtgaga gtaaaacgtc aatgttggtg
      301 catcgcgaaa aggatctaat tggcctgttg caagatctgg aatcattatc atatcactat
      361 tttcaacttt acgaaaacca taactggatc cgtcaaatcc aacaccttcg cttaaaagtt
      421 tttcaagtat gtttggataa acaggaagtg tgatgtggtg taatctgccg gtaagatcaa
      481 ttgcttttaa atctataaac tctattttat tttttttgat tagggattta caattttcaa
      541 gatgattgtt tttcattttg atctctgatg attaataaat gcattattta ttatacctga
      601 ttatagacaa aaatgcccga ctgttgaaac aatcgggcaa ataaataatt aaccgttata
      661 atttaaagcg gcaacaccag gaaagattcc tgttgtatcc aattcatctt caattcttaa
      721 aagctgatta tattttgcga tacgatctgt tcttgaagct gaaccagttt taatttgtcc
      781 cgcatttgta gcaacagcaa tatctgcaat ggttgtatct tcagtttcac ctgaacgatg
      841 gctgataacg cttgtgtaac cattaatttt tgcaagctca attgcatcaa gtgtttcagt
      901 taatgttcca atttgattta ctttaataag aattgaattt gcaactccaa ggtcaattcc
      961 ttttgctaaa cgatttgtgt ttgttacaaa taaatcatca ccaacaagct gaatttttcc
     1021 gccaagtttt tcggtcatca acttccatcc atcccaatca tcttctgcta atccatcttc
     1081 aatagaaact attggatatt gctttgccca gccggcccag taatcaacca tcttttcgat
     1141 cgggattaat tggtttggag ctgatttaaa tagatgataa gcattttttt ctttgataaa
     1201 gaattcactt gctgcaggat cgagtgctat accgatatct tttccaacct tgtaaccagc
     1261 tttttcaata gcttcaagaa ttacttcaat agcttcttcg tttgatttta aatttggtgc
     1321 aaatccgcct tcatcaccaa ctgcggtatt ataacctttt tttgcaagta ctgattttaa
     1381 tgcgtgaaaa gtttctgcgc ccatccttag agcttcttta aaacttggag ctccgattgg
     1441 cattaccata aattcctgga agtcaacatt attatcagcg tgcttaccac cgttaagaat
     1501 at
//
LOCUS       DASWBY010000671         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_102533, whole genome shotgun sequence.
ACCESSION   DASWBY010000671 DASWBY010000000
VERSION     DASWBY010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102533"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..620)
                     /locus_tag="VF870_12200"
     CDS             complement(201..620)
                     /locus_tag="VF870_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX9252999.1"
                     /translation="MKKAVIVIQNKNSTTKKYAEEIAQFLLNRGLTAELIPINNFEPK
                     KLEEADYLLVSGWKNGSLFSAKHPDSEWVSFVNKLPTLNGIKTALFTTYHLFSHGIVK
                     NMKKYLGRKTDNLEFAFTSRDGSLTISDKLTLNDFIG"
     gene            927..1265
                     /locus_tag="VF870_12205"
     CDS             927..1265
                     /locus_tag="VF870_12205"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HEX9253000.1"
                     /translation="MIQKRNMLAQVGLVIITFGIYAIYWFYKISEEMKYVGKDAEASP
                     GLWTFLLFIPLANIWSYYKFSELYEKVSSDRFNKWLLFVLWLVFSPAVWFIVQTEMNK
                     KADQFLVQNI"
     gene            complement(1412..2137)
                     /locus_tag="VF870_12210"
     CDS             complement(1412..2137)
                     /locus_tag="VF870_12210"
                     /inference="COORDINATES: protein motif:HMM:NF028342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX9253001.1"
                     /translation="MLILGVQEKQLLSKPINVYCPNCKSENTSKIKVAVNYFNLFWIP
                     VFPFNKKVKVDCSACNFQTDLDGMPEEFKDKIDKLKNSIKTPKWLFTGTLLIILAAMF
                     FTFESIRKAGIEKLINAPMAGDVYEIKTQDNYYSLLAIVKVSGDSVFVCFHNYQYENR
                     AGLTELHKTNDFNKQAYLYYKDELIYNFRDGLILNIIRGTTSLDLEYVIQEEPKINPD
                     NAKKYLDDQSGPRRSQSAKPAHK"
     gene            complement(2258..2647)
                     /locus_tag="VF870_12215"
     CDS             complement(2258..2647)
                     /locus_tag="VF870_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253002.1"
                     /translation="MEEEELFCSVCNRDIKEEDDICPHCGSDLVEFEDESPSERKYHI
                     LIRIRESIKILMAVLGAALFIGLLYGIFLKDMDIIMYTIVPIIDIGMLLIFFELIKVF
                     IDLEQNSRTQTRLLNKLLKNVSSGKQV"
BASE COUNT          818 a          447 c          412 g         1010 t
ORIGIN      
        1 tcccgtagag tgtcttcaga atattttatg tttctgatgc aaatttaatt aaagctgtct
       61 tgttagaaaa taacgctgcg gtttttaagt gagaattatt aaacagtagt tgtttgaatt
      121 gtcctgcgaa ctttgtggta aaatctttac cacaaagaac acagctttat caccaaaaaa
      181 atctggtgga tatatgtttg ctatcctata aaatcgttca atgtcagctt atcagaaatt
      241 gtaagactac catctcttga tgtaaaagca aattctaaat tgtcggtttt acgtcccaaa
      301 tattttttca tgttttttac aattccatgt gaaaataaat gataagttgt aaacaaagcc
      361 gtctttattc cgttcagtgt tggcagttta tttacgaagc ttacccactc gctgtcggga
      421 tgctttgcag aaaataatga accgtttttc catccgctta ccagcagata atctgcttcc
      481 tcaagttttt ttggttcaaa gttattaatt ggtataagct ccgcagttag tcctctgtta
      541 agcaggaact gtgcaatttc ttcagcatat ttttttgttg tgctgttttt attctggata
      601 actattactg cttttttcat ttctacacct ttcagaaatt tttctggagg aaacttaaaa
      661 agcatcacat caaaacacta tcaacttatt atgagcggag attattagtg atatatcctg
      721 aattattgcc tctgagcaga tcgtttaatt ttatcataag aataatggaa tatggtaaag
      781 tcatctcttt aggaacattg catttacaaa taatattaaa atttgattaa catatctctt
      841 ttactatttt tattgagcaa ttggtcttat ttatcctggg gtttgctcta taatctattt
      901 tacagtaata tcagaaggaa attaaaatga tccaaaaaag aaatatgctt gcacaagttg
      961 gactggtgat catcacgttt ggcatttatg caatttactg gttctataaa atttctgaag
     1021 agatgaaata tgtaggcaaa gatgctgaag cttctcccgg gctttggaca ttcttgcttt
     1081 ttattcccct ggctaatata tggtcgtact ataaattttc tgagctctat gaaaaagtat
     1141 cttcagatag attcaacaag tggctgttgt tcgttctgtg gcttgtattt tcgcccgcag
     1201 tgtggtttat tgttcaaaca gaaatgaata aaaaagcaga ccaatttctt gtacaaaaca
     1261 tttaatctgt taaaactgtt tatcaataaa agccttccgt aaactggagg gcttttttat
     1321 gaaaagaata tttgtggtat cgtgatggtt atttcttttt aacagcacgt tgtacttcaa
     1381 acttacgctt taatatttaa ttcgctttat actatttatg tgcgggtttt gctgattgag
     1441 atcttcttgg tccagattga tcatctaaat acttttttgc attatccgga tttattttag
     1501 gttcctcttg aataacatac tcaagatcta aactagtggt tccccttata atgtttaaga
     1561 taagtccatc tctaaagttg taaataagct catctttata atatagatat gcttgcttat
     1621 taaaatcatt tgttttgtgc agctctgtta aaccggccct gttctcgtat tgataattat
     1681 gaaaacaaac aaaaacagaa tcaccgctta cttttactat cgcgagaaga gaataataat
     1741 tatcctgtgt tttaatctcg taaacatctc ccgccattgg tgcgttaatt aatttttcga
     1801 taccagcttt tcttatactt tcgaaagtaa aaaacatagc ggccagtata atcaaaaggg
     1861 tccctgtaaa aagccacttg ggggttttaa tgctgttctt tagtttatct atcttatctt
     1921 taaattcctc cggcatacca tctaagtccg tttgaaagtt acatgcagaa cagtcaacct
     1981 taactttttt attaaacgga aataccggaa tccagaataa attgaaataa tttactgcaa
     2041 ctttaatttt gcttgtgttt tctgatttgc aattaggaca ataaacatta attggttttg
     2101 acaataattg tttttcttgc acaccaagta ttaacataat tgtatttcct ggctgtttat
     2161 tagtaattca attatagctt agcatcaacg atacagaatg taagtaattc aataaatatt
     2221 tagactgttt ttaattcaca taaattattt aacttattta aacttgttta ccggatgaaa
     2281 cgtttttcag gagcttattc aataaccttg tttgagttct cgaattttgc tctaagtcaa
     2341 taaacacttt gattaattca aaaaatatca gaagcattcc tatgtctatg atggggacaa
     2401 tcgtatacat tataatatcc atatctttta ggaagatccc atatagtaat ccgataaata
     2461 gagcagctcc taaaacagcc attaatattt taatgctttc tcttattctt ataagaatat
     2521 gatattttct ttccgaaggt gattcgtctt cgaattcaac cagatctgaa ccacaatgag
     2581 gacatatatc atcttcttct tttatgtctc ggttacaaac ggaacaaaac aattcttctt
     2641 cttccatgtc tttccctttt ttttgttaag attgtttttc tactttt
//
LOCUS       DASWBY010000672         5535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_102557, whole genome shotgun sequence.
ACCESSION   DASWBY010000672 DASWBY010000000
VERSION     DASWBY010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102557"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VF870_12220"
     CDS             complement(<1..168)
                     /locus_tag="VF870_12220"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX9253003.1"
                     /translation="MKNFELVSSYKPSGDQPEAIKQLVEGVNRGDKFQTLLGVTGSGK
                     TFTISNVIVQTN"
     gene            complement(175..513)
                     /locus_tag="VF870_12225"
     CDS             complement(175..513)
                     /locus_tag="VF870_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253004.1"
                     /translation="MKILQKLILPLLLVLIVVIIYKFYFAKSGLGSFDTFDPNNTAVK
                     EIRVQLVIDRGITQQGEDVVFYASDKNGKIMMVTGHVTLPQGFEQAETIILKGHLSGN
                     SFHTHEVLLD"
     gene            598..969
                     /locus_tag="VF870_12230"
     CDS             598..969
                     /locus_tag="VF870_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX9253005.1"
                     /translation="MNDKKLWLIISAISGFTAVAIGAFGAHGLREKLSSEMLEVYKTG
                     VLYQLIHSVVLLVLSLNTLIKAKLPSIFILLGIILFSFSLYIYSTSGIRFFAMITPVG
                     GFCFLIGWLLIIYEALKLKQT"
     gene            complement(1013..1567)
                     /locus_tag="VF870_12235"
     CDS             complement(1013..1567)
                     /locus_tag="VF870_12235"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9253006.1"
                     /translation="MIKLNLGCGKDIKDGYINLDIVDYGGNQIHDINTFPYPFPENHF
                     NEIYASHVLEHIDNFNKTITELYRILKPNGIMIVYAPFFLNTKYFGDPDHKIPFSIRT
                     FDNYEYIGKRKLKFYEKWKLNHRTNYETGAQFEVLEKKFITSHFAALKWMDFLVNIEP
                     VMYERFFSGIFSPEEVYFKLRAIK"
     gene            complement(1678..2097)
                     /locus_tag="VF870_12240"
     CDS             complement(1678..2097)
                     /locus_tag="VF870_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253007.1"
                     /translation="MKKSFVLFAVLFALVLGLSQNILAQNEPVLYFCEKYGKDGEVGV
                     SDRFTTGYLTVMVKCDYALDLEDCSIQFDLYNPRTKEFEYYKKFSYVVEPDMKYIYFA
                     KNDESDLKFEDPGFYRVFLLDDRDKTVASALIEIIDK"
     gene            2529..3215
                     /locus_tag="VF870_12245"
     CDS             2529..3215
                     /locus_tag="VF870_12245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9253008.1"
                     /translation="MNKILPKKKWNNLKQKPIIKHCCFDLDGTLVDSNKTIYEATAYS
                     LEKLGIDFNVDEEAFALKIGQHFVDIFNAFSIDVPDFEKFITIYKDNYFEQMEYSKVY
                     NGVEKTLASLKDQDIKVSLLTTKIQDQADSIIDHFKMRKYFDLVMGRRDGIAHKPSPE
                     PLLKICDDLSIDVKSTLMVGDTELDIQCGKNAGSYACGVLYGYRTKELLEIENPDFIV
                     ENIEDILKIL"
     gene            complement(3288..4337)
                     /locus_tag="VF870_12250"
     CDS             complement(3288..4337)
                     /locus_tag="VF870_12250"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX9253009.1"
                     /translation="MLSKIKELLGSEADSLLSYKAKFPKEQLHLPGPDFVDRIFSQTD
                     RNINVLKNLEWIFQTGRLAGTGYVSILPVDQGIEHSAGASFAPNPVYFDPENIVKLAI
                     EGGCNAVASTLGVLGMTARKYAPKIPFIVKINHNELLTYPNKFDQIMFANVEQAYDMG
                     AAAVGATIYFGSEESNNQIIEVSEAFHYAHELGMATVLWCYLRNNAFKSDKDYHVSAD
                     LTGQANHLGVTIEADIIKQKLPENNGGYNSIKGFGKTHKKVYTDLTTDHPIDLTRYQV
                     INNYMGRAGLINSGGASGENDFAEAAKTAVINKRAGGMGLISGRKAFQRPMSEGVQLL
                     NLIQDVYAEKEITVA"
     gene            complement(4509..4994)
                     /locus_tag="VF870_12255"
     CDS             complement(4509..4994)
                     /locus_tag="VF870_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX9253010.1"
                     /translation="MRSLTKILFVLVLVALVGFTLVVNEDKIIEPVVTDNVVSVEEET
                     VTADRSLVEFVDKGSMKASWYGPGFHGRKTANGEVYDQMSFTAAHKSLKFGTLLKITN
                     LKNNKSVVVRINDRGPYVDGRDLDLSKAAALELGMVRKGVAKIKVEEIKIAGLENLMM
                     N"
     gene            5258..>5535
                     /locus_tag="VF870_12260"
     CDS             5258..>5535
                     /locus_tag="VF870_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253011.1"
                     /translation="MNSTQKRGIIFLTAVFITTFFIGGSTRLPVTDENENFYLSNYTT
                     FAPGEIAKLNLYSYSQSTKKFSLKLLKIEDPVRFFSLLDQNNSRYAFD"
BASE COUNT         1798 a          978 c          895 g         1864 t
ORIGIN      
        1 atttgtctga acaattacat ttgaaatcgt aaaagttttc ccgcttccgg taacaccaag
       61 taaagtctgg aacttatccc cacgattaac accttcaact agttgtttaa ttgcttcagg
      121 ctgatctccg gatggtttgt agctggaaac aagttcaaaa tttttcatag attattaatc
      181 gagtagaact tcatgagtat gaaaactatt gccgcttaaa tgacctttaa gaataattgt
      241 ttctgcctgt tcaaatcctt gcggcaatgt aacatggcca gtaaccatca taatctttcc
      301 atttttatcg gatgcgtaga acacaacgtc ttctccctgc tgtgtaatac cacgatcaat
      361 aacaagctga acccgtattt ctttaactgc agtattgtta gggtcaaaag tatcaaatga
      421 tcccaatccg ctttttgcaa agtaaaattt ataaattatg actacaatta gaaccaataa
      481 taaaggcaaa atcaattttt gtaatatctt catttctttt cttcattgtt attaaattgt
      541 gatcaaagtt aataaaattt gtttttacta ttcaactcaa tttaaggtta gaaatttatg
      601 aatgataaaa aattatggct tatcatttct gctatttccg gattcacagc cgttgcaatt
      661 ggtgcttttg gagcccacgg attgagagaa aaactttcat cagaaatgtt ggaagtctac
      721 aaaacaggag ttttgtatca attaattcat tccgttgtat tgcttgttct ttcgttaaat
      781 acacttatta aagcaaagct tccatcaatc tttatattgc tgggcataat tttattttca
      841 ttttcacttt atatctattc aacatcagga ataagatttt ttgcaatgat aactcctgtt
      901 ggcggttttt gcttcttgat aggctggctg ctgattattt atgaagcttt aaaattgaag
      961 caaacctgaa ttaccactta gacacttaga gcgccaagca gcacctggta ttttatttaa
     1021 tcgctcttag tttaaagtaa acttcttcag gagaaaatat cccggaaaaa aatcgctcgt
     1081 acataacagg ctctatgtta accagaaaat ccatccactt aagtgcagca aaatgtgagg
     1141 ttataaattt cttttccaaa acttcaaact gagctcctgt ttcataatta gtgcggtgat
     1201 ttaatttcca cttttcataa aatttcaatt tacgcttgcc aatgtattca tagttatcaa
     1261 aggtacgtat tgaaaatgga atcttatgat ctggatcgcc aaagtatttt gtgtttaaaa
     1321 agaacggagc atacacaatc ataattccat tcggttttag aattctgtac agctcagtaa
     1381 ttgttttatt aaagttatca atatgctcaa gcacgtgcga agcataaatc tcattaaaat
     1441 gattttccgg aaaaggatat gggaaagtgt ttatatcgtg gatttgattc cctccgtaat
     1501 cgacaatatc aagattaatg tatccatctt taatatcttt tccgcagcct aagttcagtt
     1561 taatcattat tactctttat aattttataa actaatttaa aacatattta tgagaaactt
     1621 agatttaaaa taaaaaaggc tgtctattaa agacagccta aataaaatag tagaaattta
     1681 tttatcaata atttcgatca atgcacttgc aacagttttg tctctgtcat caagtaaaaa
     1741 tactctgtaa aaaccaggat cttcaaactt taaatcgctt tcatcatttt ttgcgaagta
     1801 aatgtatttc atatccggct ccactacata actaaatttt ttgtagtact caaattcttt
     1861 tgttcgtgga ttgtaaagat caaattggat tgaacaatct tccagatcaa gtgcgtagtc
     1921 acatttaacc attacagtta aatatccggt ggtaaatcta tcacttacac caacttcacc
     1981 atctttgccg tacttttcgc aaaagtaaag aacaggttca ttttgggcga ggatgttttg
     2041 acttaagccg agaacaagag caaagagaac tgcaaaaagc acgaacgatt ttttcatata
     2101 tagctcctta ttttatttat gtgagtatta atacacaaaa tattgtgcca ttaaattatt
     2161 tagttatttt tataaaatta aaacagacct gaaaatattc tcacctaaat attctttttg
     2221 agatagaatt tacgggtaat gaaaactctt gggattgagt tatatgctca tcttttgaaa
     2281 aaccctgtat ttttcttagc gagaaaattg tatttttcaa aataaaaaca aagtggtttt
     2341 ataaatatat ttttatcctt ttatttagaa tttagtctcc atgcaagaat ttaataagac
     2401 accaaattca ctttgccaat ggtgcgggca gccaaatcat ataatttggg ttcatggaca
     2461 tgggcagtgt tccgtttctg gatataatat agaagaatgc cgcaaagggg aaatttgtga
     2521 acaacccaat gaacaaaatc ttacccaaga agaaatggaa taacttgaag caaaaaccga
     2581 ttattaaaca ctgctgcttt gatcttgatg ggaccctggt agattctaac aagactattt
     2641 atgaagcaac cgcctactca ttagaaaaac ttggaattga ttttaatgtg gatgaagaag
     2701 cttttgccct aaaaatcggg cagcattttg tagatatatt taatgctttt agtattgatg
     2761 tacctgattt cgaaaaattt attacaattt ataaagacaa ttacttcgag cagatggaat
     2821 actcaaaggt ttataatgga gttgaaaaaa ccttagcttc tcttaaggac caagatataa
     2881 aagtttcgtt gcttactaca aaaattcagg atcaggcaga tagtattata gatcatttta
     2941 agatgagaaa atattttgat cttgttatgg gtaggcggga cggaattgcg cacaagccat
     3001 ctcctgaacc tttattaaag atttgtgacg accttagcat agatgttaaa agcacactaa
     3061 tggttggaga tacagaactt gatattcaat gtggtaaaaa cgcgggttca tacgcttgtg
     3121 gagttttata cggctacagg acaaaagaat tgctggagat cgaaaatccg gattttatag
     3181 tcgagaacat tgaagatatt cttaagatac tttaaaatta aaaaggcgag caattactcg
     3241 cctttaataa tttatccctc tttattaaaa gggaatataa agatcaacta agctacagta
     3301 atttcctttt cagcataaac atcctgaata aggtttaata attgtacacc ttcactcatt
     3361 gggcgttgga aagctttacg tcctgagata agtcccattc cgccggcacg tttgtttatt
     3421 actgctgttt tagctgcctc cgcaaaatcg ttttcaccgg aagcaccacc gctgtttatc
     3481 aatcctgcac gacccatata attattaata acctgataac gggtaagatc gatcggatgg
     3541 tctgttgtta aatccgtgta aaccttttta tgggtttttc caaatccttt aatggaattg
     3601 tatccgccat tattttcagg aagcttttgt ttgatgatat cagcttcgat tgtaacgccg
     3661 agatgatttg cctgtcctgt taaatctgca gatacatgat aatctttatc tgatttgaaa
     3721 gcattgtttc tcaggtagca ccataaaact gtagccattc caagttcatg tgcatagtgg
     3781 aatgcttcac taacttcaat tatttgattg ttactttctt cagaaccgaa ataaattgta
     3841 gcgcccactg cggcagctcc catatcatat gcctgctcaa catttgcaaa cataatttga
     3901 tcaaacttgt ttggataagt aagtagttcg ttgtggttaa tttttacgat gaaaggaatt
     3961 ttgggagcat atttccttgc cgtcattcca agtacaccaa gagtagaggc aacagcatta
     4021 caaccgcctt caattgcaag ttttacaata ttttcaggat caaagtaaac cggatttggt
     4081 gcaaaggatg cacccgcgct gtgttcaatt ccctgatcaa ctggaagtat tgaaacatat
     4141 cctgttcctg ctagtcttcc ggtttgaaaa atccactcca gattttttaa aacattaata
     4201 tttctgtccg tttgagaaaa gattctgtca acaaaatccg gcccgggcag atgtaattgt
     4261 tcttttggaa atttagcttt gtaggataac agggaatcag cttcactgcc taacaattct
     4321 tttattttac ttaacattaa agctccattt tttaaatgat ttatattatt tgttcatatt
     4381 tgccaaacta aaataagcta agcgacatag taattcaatt taaatttggt tcaagaggta
     4441 ataatctgtt taaaaataaa aaacccggtt ggttccgggt tttaatgtgt aatgtataat
     4501 gagtcttttt agttcatcat cagattctct aatcctgcaa ttttaatttc ctcaacctta
     4561 atctttgcaa cgccttttcg aaccattcca agctctaaag cggctgcttt agaaagatca
     4621 agatctcttc catcaacata aggacctctg tcatttattc taacaacaac agatttattg
     4681 ttttttagat tagtaatctt taataaagtg ccaaatttaa gcgatttatg agctgcagta
     4741 aaggacatct gatcataaac ttcgccgttt gcagttttgc gtccatgaaa acctggaccg
     4801 taccaagaag ctttcatact tcctttatct acaaactcaa ctaaagaacg atcagcggtt
     4861 actgtttctt cctcaactga aaccacatta tcagttacaa ccggttcaat gattttatct
     4921 tcatttacaa ctaaagtaaa accaaccagc gcaactaata caagaacaaa taatatcttc
     4981 gttaaagacc tcaatagaac ctctaattta tgaaacatat cggattgctg atacaaattt
     5041 aatccgggga attagaccat ccaatacttt atttggttct ggagtccacc ggatagaaat
     5101 ccaatcattc gactattatg gtattaaagt ctgaaatgat gttccaacta gtaccttgat
     5161 cagttaatag cagactagga attatgtaat tgctcaataa gacattggca cttttattat
     5221 ttaagtttgt ataagactct aaattctgga gaaaaatatg aattcgactc aaaaaagagg
     5281 aataattttc ctaactgcag tttttattac aacttttttt atcggtggct ctaccagatt
     5341 accagtgact gatgagaatg agaacttcta tctttcaaac tacacgacgt ttgcgccggg
     5401 agaaattgct aagctaaatc tctactctta ctcccagagc acaaaaaagt ttagcttaaa
     5461 gttattaaag atagaagatc cggtcagatt tttctccctt ttggatcaaa acaatagccg
     5521 ttatgctttt gatat
//
LOCUS       DASWBY010000673         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_290805, whole genome shotgun sequence.
ACCESSION   DASWBY010000673 DASWBY010000000
VERSION     DASWBY010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290805"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VF870_12265"
     CDS             complement(<1..415)
                     /locus_tag="VF870_12265"
                     /inference="COORDINATES: protein motif:HMM:NF025115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HEX9253012.1"
                     /translation="MKKIFILFYLMSFSCLLLANNYIIADKDFNKADTLEIKTLNYSK
                     STKDSTAIINIDYPQIEGLENAAIERKVNLFLEEEFKQAIAWFDEVQSDTAFFEDFPS
                     EMQYTFETGFQTEFNSKNFISIVLSHYQFTGGAHGN"
     gene            complement(438..1010)
                     /locus_tag="VF870_12270"
     CDS             complement(438..1010)
                     /locus_tag="VF870_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9253013.1"
                     /translation="MIDFLYSIDLAIFYFFNHTLSAGFLDKFFSIITNVNNWYIAYII
                     LLGISFTKGGTKGKLAVIGVILLIVITDQTGYRLLKEYFARPRPCNFLTDVLTPLGCT
                     GSYSFPSNHALNNFAAAMFFYRLFPKLKWVLFITALLISISRVYLGLHYPSDIFGGAI
                     IGLAFGYLFASGVLHLEKYLADRKIKHSES"
     gene            1108..>2488
                     /locus_tag="VF870_12275"
     CDS             1108..>2488
                     /locus_tag="VF870_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9253014.1"
                     /translation="MNKSKSLTGFLVNSSFPFFKEGYNRIKEESGKYLVTPLKVIALM
                     VAISGIFAMIFEVRHHAEFASQIYMVRLISTLIAFIILVFLSTKNAIRYAIPLVHILL
                     LSIIGSSALMILLMPNSLIVNSQIVGLMIFTSAMFLNWEIKNQILVAIYYNIVFAVAI
                     LFNDQKIYFLPNMFESLIFVLFLSLLSVVGSAINFRMRLQVAENALKVEHSEKKFRSI
                     FNNSSDGIFQSTLDGDFLTANKSLVKMFGYENLDDLIITRVPDYYKNKSDRDKLLAEI
                     KKHGTVENYFTTLVDKNKNDISVKMNIRYVTDDVTDKQYLEGTIRDVTSEVLAQERRN
                     KAELELKTEKEKSDALAREAMKLSTAKSRFLANMSHEIRTPMNGIVGFLSLIENESYQ
                     NHDELKQFVRNAKMSAESLLDTINAVLDLSKIEAGKIELENLNFNLKKIVTQAISIVS
                     PKAKEKSLNI"
BASE COUNT          887 a          393 c          389 g          819 t
ORIGIN      
        1 aattgccatg tgcaccgccc gtaaactgat aatggcttaa cactatgctt ataaaatttt
       61 tagaattaaa ctcagtctga aatccggttt caaacgtgta ttgcatttca ctgggaaaat
      121 cttcaaagaa agcagtatca gattgaactt catcgaacca cgcaattgct tgtttgaatt
      181 cttcttctaa aaacaaattc actttccgtt caattgctgc attttccaaa ccttcaattt
      241 gaggataatc tatatttata attgcagtac tgtccttagt gcttttcgag taattcaaag
      301 ttttgatttc aagcgtatcg gctttattaa aatctttatc agcaattata taattattag
      361 ctaataataa gcagctgaaa ctcatcaagt aaaagagaat aaaaattttt ttcatttatg
      421 tataaccttt taaaaaatta tgattcagaa tgtttgattt ttctatcagc caaatacttt
      481 tctaaatgca aaactccgga agcgaataaa tatccaaaag ctaatccaat tatcgcacct
      541 ccaaaaatat cggaagggta atgtaaacct aaataaactc tggatattga tatcagcaaa
      601 gcggtgataa acagaaccca ttttaacttt ggaaataatc tgtaaaaaaa cattgctgcc
      661 gcaaaattgt ttagtgcatg atttgacgga aatgaatagg atcctgtgca tccaagtgga
      721 gttaaaacat ctgtcagaaa attgcacgga cggggtcttg caaaatattc ttttaacaat
      781 ctataacccg tctgatcagt aataactatt aataaaatta caccaattac cgccagtttg
      841 ccttttgtac cgcctttagt aaaactaatt ccaagtaaaa taatataagc tatataccag
      901 ttattaacgt ttgtaattat ggaaaagaat ttatctaaaa atccagctga aagtgtatga
      961 ttgaagaagt aaaaaatggc tagatcaatt gagtataaaa aatctatcat ataaaatatt
     1021 ttgaaaattg ttaacgttaa gatagaaatg atatatcaat ttatcataaa aattctgtaa
     1081 atttcttccg cactttctga gtaaataatg aacaagagta aatctctaac aggtttttta
     1141 gtcaattcaa gttttccatt ttttaaagaa ggttataatc gtattaaaga agaatcaggt
     1201 aaatacttag ttacaccttt aaaagttatt gctcttatgg ttgctatctc aggcatcttc
     1261 gcaatgattt ttgaagttcg tcaccatgca gagtttgctt ctcagattta tatggtaaga
     1321 cttatctcaa ctcttatagc atttataatt ttagtttttc ttagtactaa aaatgctatt
     1381 cgatatgcca ttccgttagt gcacatcctt ctgctaagta tcatcggttc ctctgcatta
     1441 atgatattgt taatgccgaa ctcacttatc gttaactccc agatagtcgg gttgatgatt
     1501 tttacttctg cgatgttcct taattgggag atcaaaaacc agattcttgt ggcaatttat
     1561 tacaatattg tatttgcagt cgcgatatta tttaatgacc aaaaaatata ttttctgcca
     1621 aatatgtttg aatcgctgat ctttgtatta tttctaagtt tactttcagt tgttggcagt
     1681 gcaataaatt ttagaatgcg tttacaggtt gcagagaatg ctttaaaggt tgaacactcg
     1741 gaaaaaaaat tccgatcgat attcaataat tcatcggacg gaatctttca atcaacattg
     1801 gatggagatt ttcttacagc aaataaatct ttagtaaaaa tgtttggcta cgaaaatctt
     1861 gatgatctta taattaccag ggtgcccgat tattataaaa ataaatctga cagggacaag
     1921 ttattagctg aaataaaaaa acatggcact gttgaaaatt attttacaac gcttgttgac
     1981 aaaaataaaa acgatatttc tgtaaaaatg aatatccgtt atgttacaga tgatgtaaca
     2041 gacaaacagt atcttgaagg tacaattcgt gacgttactt cagaagtttt agcacaggaa
     2101 cggcggaata aagcagaact tgaacttaaa accgaaaaag aaaaatctga tgctctggcc
     2161 agagaagcta tgaagttaag tactgccaaa agcaggtttc ttgccaatat gagtcacgaa
     2221 atccgtacgc caatgaatgg tatagttgga ttcttatctt taattgaaaa tgaatcttat
     2281 caaaatcatg atgaattaaa gcagtttgtt cgcaacgcaa aaatgtctgc ggaatcatta
     2341 cttgatacaa taaatgcagt tcttgattta tcaaaaattg aagcaggaaa aattgagctt
     2401 gaaaatctta acttcaatct taaaaaaatt gtaactcagg caatttcaat cgtgagccca
     2461 aaagcaaaag aaaaatcatt aaatattt
//
LOCUS       DASWBY010000674         3677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_623979, whole genome shotgun sequence.
ACCESSION   DASWBY010000674 DASWBY010000000
VERSION     DASWBY010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623979"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..875
                     /locus_tag="VF870_12280"
     CDS             159..875
                     /locus_tag="VF870_12280"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX9253015.1"
                     /translation="MRLSLNIDHIATLRNARGETQPDPVTAALLAEQAGIDGIVVHLR
                     EDRRHISERDVRLLRELITTKLDLEMAATEEIIKIACDIGPELATIVPEKRQELTTEG
                     GVNVLDNITLMKNTIEELHKYDIEVSLFIEPDIRQIDAAAEIKSDYIEIHTGVFANAL
                     SEEEQFDELDKIRVAVKHAKKLGLGVNAGHGLDYQNIKIFRELTDIDEVSIGHSIIAR
                     SVFVGIKQAVKEMFDLINKS"
     gene            complement(872..1525)
                     /locus_tag="VF870_12285"
     CDS             complement(872..1525)
                     /locus_tag="VF870_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX9253016.1"
                     /translation="MATLKTFNHLVSFALVLSLITIFYNIAEGLFSIFFGLNDETLAL
                     FGFGLDSFVEVISGIGILHMIIRMKRNPVTKRDGFERTALKITGFSFYLLCAGLIIAS
                     VLLIINDMKPETTIIGIVISTISILTMYVLLYYKLKVGKALNSDAIIADANCTKTCFY
                     LSFILLASSLSYEVFKIGYLDIAGSLGIAYFAFKEGKESIEKSKSEKLSCSCEDNCH"
     gene            1873..>3677
                     /locus_tag="VF870_12290"
     CDS             1873..>3677
                     /locus_tag="VF870_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9253017.1"
                     /translation="MQYNLIKKSEFDKVKTFKGNWTAKMELFADMCRYNTLVAVKKAG
                     SGHLGSSLSAMDITTYLYLNEMNVFDIGFDSPDRDIYFSSKGHDVPGLYALFYALGII
                     PEEKLLMLRRLHGLDGHPEVRQNGIEASTGSLGMGISKGKGFAWAKSYQKNKGHVFVL
                     TGDGEFQEGQIYESLQATAHQKVNNITAIMDHNKYQTDMLVADVNNIEDVVAKVEAFG
                     WHVVRINGHDYNILKKTFDDLKKVTDKPKMIIADTIKGRGVSFMEKPLTETFQGKTLY
                     KWHSGAPDDESYEKGLAELTETINKLSDELGIGKIQVPENKSEGKTITKLDKEFVTEA
                     YGEALVEIAKTNKRLVVLDGDLSADCKLRNFEKTYPDRFIENGIAEQDMVSMAGGLAR
                     MGLIPVVNTFASFLAARANEQIYNNAGEKTKIIYTCHFSGMIPAGPGKSHQSVRDISL
                     FGALPNITIIQPCNGEETKWATEYCVNIAEDNCMLRLVIGPSPEKIELPKDYKFKIGV
                     GAQLTEGNDAILFGYGPVMLHEALVAADYLKKIGFGLKVINMPWLNKIDSEWLKEIVK
                     NQKRIFVLEDHSAFGGLGDKILNVLVETNSIAGKE"
BASE COUNT         1297 a          552 c          700 g         1128 t
ORIGIN      
        1 gcattaagaa ctcgttcgat ttattattgt atcgttgttc atataggtgt aatgttttcc
       61 atagatttaa tttcatcttt acgttttcgt gctgatgatt acggaattgg gttttcatct
      121 atcttaaata tattaaagca aattttctga ggtatgaaat gagattatca ttaaatatag
      181 atcatatagc aacattaaga aacgccaggg gagaaacgca gcctgatccg gtaactgcag
      241 cattattagc agaacaagca ggcattgatg gaattgttgt tcatctgcgt gaagatagaa
      301 gacatatcag cgaaagagat gttagattgc ttcgtgaact tatcacaaca aaacttgatt
      361 tagaaatggc tgcaactgaa gagataatta aaatagcttg tgatattgga cctgaacttg
      421 ctactattgt accggaaaaa agacaagaac taactaccga aggcggtgta aatgttctgg
      481 ataatattac cttaatgaaa aacaccattg aagagcttca taaatatgat attgaggttt
      541 ctcttttcat tgaaccggat ataaggcaaa ttgatgctgc tgcggaaatt aaaagtgatt
      601 acatagaaat acacaccggt gtttttgcta acgctctttc tgaagaggaa caatttgatg
      661 agttggacaa aatcagggtg gctgtaaagc atgcaaagaa attaggatta ggtgtaaatg
      721 caggtcatgg tttggattat cagaacatca agatttttag agagttaacc gatattgatg
      781 aagtaagcat cgggcattca attattgccc gttcagtttt tgtagggata aaacaagctg
      841 taaaagaaat gttcgatctg attaataaaa gttaatggca attatcttcg caggaacagg
      901 atagtttttc gcttttagat ttttctatgg attccttacc ttctttaaaa gcaaagtacg
      961 caattccaag ggaacctgcg atgtcaagat atcctatttt aaaaacctca taacttaaac
     1021 ttgatgctaa caggataaat gacaaataaa aacaagtctt agtacagtta gcatctgcta
     1081 taattgcatc ggagtttagt gcttttccaa ctttcagttt ataatataac agaacataca
     1141 tagttaaaat tgaaatggtt gatattacta ttccaatgat agttgtctca ggcttcatat
     1201 catttataat taatagtacc gaggcaatta ttagtcccgc acaaagtaag taaaaactaa
     1261 agccggtaat ttttagagca gtcctttcga atccatccct ttttgtaacc ggatttcttt
     1321 tcattcttat tatcatgtgt aaaatgccaa tcccagaaat aacttcaaca aagctatcca
     1381 atccaaaccc aaaaagagcg agagtttcat cattcaatcc aaagaaaatg gaaaataaac
     1441 cttcggcaat attataaaaa atggttatta ggcttagaac aagagcgaat gaaactaagt
     1501 ggttaaatgt ttttaaggta gccatattag agcttttaat gtctttccaa tcattttaat
     1561 tgcgcataat aaaatatttt tatttataaa ttactaacat cagagagata ttgaaaatat
     1621 ttttaattat ttctcaaaaa tctattgatt aattaaaaat taaaatttaa gtttcgggtg
     1681 tcgctagcag aaggttaacc cccagacctt ttgttaggac aagcccggta gcttcggtta
     1741 ccgggttctt aattttaaag ccctcaatgt ttttaatcac gtaacgatgt cttaattatc
     1801 ttttttaaaa tcataaacct atttttctat tatactttct aaaacataac atagaaaata
     1861 ttcggaggcg ctatgcaata caaccttata aaaaaatcag agtttgataa agtaaaaaca
     1921 tttaaaggca actggactgc aaagatggag ttatttgcag atatgtgcag atataacact
     1981 cttgttgctg taaagaaagc tgggtcaggg caccttggtt caagtttaag tgcaatggat
     2041 attacgactt atctatactt aaacgaaatg aatgtatttg atattggatt tgattcacct
     2101 gatcgtgata tttatttttc atcaaaaggt catgatgttc cgggattgta tgcattgttt
     2161 tatgctctcg gaattattcc ggaagaaaaa ttattaatgc taagaagatt gcacgggctt
     2221 gatggtcatc ctgaagttag acaaaatgga attgaagcca gcacaggttc gcttggaatg
     2281 ggaatttcta aaggaaaagg atttgcctgg gcaaaatctt atcaaaaaaa taaaggtcat
     2341 gtttttgttc ttaccggtga tggagaattt caggaaggac agatttatga atcacttcaa
     2401 gcaacggcac accaaaaggt aaataatatc actgctataa tggatcataa taaatatcaa
     2461 acggatatgc tggttgctga tgtaaataat attgaagatg ttgtagcaaa ggttgaagct
     2521 tttggatggc acgtagtaag aattaacgga cacgattaca atatcttaaa aaagactttt
     2581 gatgatttga aaaaagttac agataaacca aagatgatta ttgcagatac cattaaaggc
     2641 aggggagttt cattcatgga aaaaccactg actgaaactt tccagggaaa aacactttac
     2701 aaatggcact ccggcgcacc tgatgatgaa agctatgaaa agggattagc tgaattgact
     2761 gaaactatca acaagctttc agatgaactt ggaataggaa aaattcaagt acctgaaaat
     2821 aaatctgaag ggaaaaccat tactaaactt gataaagaat ttgttacaga agcttacggt
     2881 gaagcattag ttgaaatcgc aaagacaaat aaaagattag ttgtacttga tggtgatctt
     2941 tctgccgatt gcaaactgcg taattttgaa aagacttatc ctgatcgatt tattgaaaac
     3001 ggaattgctg aacaggatat ggtttctatg gcaggcggat tagcaagaat gggattaatt
     3061 cctgttgtta atacatttgc aagtttccta gctgcaagag ccaacgaaca gatttacaat
     3121 aacgctggag agaaaactaa aataatctac acctgtcatt tttcaggaat gattcctgca
     3181 ggacctggaa aatctcatca aagtgttaga gatatttcgt tatttggggc gctccccaat
     3241 attacaatta tccagccttg caatggggaa gaaacaaaat gggcaacaga atattgtgtg
     3301 aacatagctg aagataattg tatgctaaga ttagtaattg gtccctcacc agaaaaaatt
     3361 gaacttccca aagattataa attcaaaatt ggtgtaggtg cacaattaac tgaaggaaat
     3421 gacgcaattc tatttggcta cggaccagta atgctgcacg aagctttagt tgcggcagat
     3481 tatttaaaga aaattggttt cggattaaaa gtaattaata tgccctggtt aaataaaatt
     3541 gatagtgaat ggctcaaaga aatagttaaa aatcaaaaaa gaatttttgt acttgaggat
     3601 cattctgctt ttggcggcct tggtgataaa attctaaatg ttttagtcga gacaaactca
     3661 attgccggca aagaatt
//
LOCUS       DASWBY010000675         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_663748, whole genome shotgun sequence.
ACCESSION   DASWBY010000675 DASWBY010000000
VERSION     DASWBY010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663748"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VF870_12295"
     CDS             complement(<1..640)
                     /locus_tag="VF870_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253018.1"
                     /translation="MAEKKVLLPASQRAILFAQATREYKVDQPAKIYSSGQTVRFDLP
                     KSRFLSKILLRVKGTFKAKHATKTSFVEMGLGRYNILNQIRISINNGFNPYQLSGKSL
                     KAYNMVSKFQKPQGDNFGVEVFENVVSVAGATNKVNFTMELPLTLNERDCVGLLMLQN
                     DETYVTVEIDLGSILSCMVDSDIVIESENITITPVLHTFSIPRDENAIPDYSV"
     gene            complement(677..808)
                     /locus_tag="VF870_12300"
     CDS             complement(677..808)
                     /locus_tag="VF870_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253019.1"
                     /translation="MKLGMRSFLMVGMMAVLFIVVSKVLVNKYSVVPQGLKDVVNAV"
     gene            complement(822..1448)
                     /locus_tag="VF870_12305"
     CDS             complement(822..1448)
                     /locus_tag="VF870_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253020.1"
                     /translation="MQIVINKDEHVFDCGRSGTGKSKLAEIYTAGMDNVIVLDTKGDF
                     KYSQLPNVPVFESLEELQTFKEGKAIYRPNIQELNSTYYELFFEWIYFRKNCTCLVDE
                     ILDVCPNSFSIPFYAKAILTRGRSRNTQMWGCTQRPKGIPTVFLSEAIHYFVFDLNTK
                     DDRKRIVENTGSTVFENNPNDKSLGEEYFFYYYNVKHMEIPIKARLKL"
     gene            complement(1432..1560)
                     /locus_tag="VF870_12310"
     CDS             complement(1432..1560)
                     /locus_tag="VF870_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253021.1"
                     /translation="MAKFCSSLDDEKTEEIIKITKQFIFEIENENVIEADVIANSN"
     gene            complement(1553..>1991)
                     /locus_tag="VF870_12315"
     CDS             complement(1553..>1991)
                     /locus_tag="VF870_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253022.1"
                     /translation="KKNVKVEETKVDAIAEISTLIQTLTVIASLKFGEHWTIDEAESN
                     SIATPLNSILNKFNLVDKIAGVSDGVALVVATVSIAIPRIIIQSQLNSQKSNKQMKVI
                     KANGGMKDDTENSSTTISNTENNVGGDTSSITSDGQFIKAISG"
BASE COUNT          598 a          352 c          250 g          791 t
ORIGIN      
        1 taactgaata atctggaata gcattttcat cacgtggaat tgaaaatgta tgaagtactg
       61 gtgttatagt aatgttttca gattctatta caatatctga atctaccata catgataata
      121 ttgaacctaa atcaatttca actgttacat aagtttcatc attttgtaac ataagaagtc
      181 cgacacaatc tctttcattt agtgttaatg gtaactccat tgtgaaatta actttatttg
      241 ttgctccagc tacagaaaca acattttcaa aaacttctac accaaaattg tcaccttgtg
      301 gtttttgaaa tttactaacc atgttataag cttttaagct tttaccactt agttgatatg
      361 ggttaaaacc attattgatt gatattctaa tttgatttaa aatattatac ctacctaatc
      421 ccatttcaac aaatgatgtt tttgttgcat gttttgcttt gaatgttcct ttgaccctta
      481 atagaatttt agaaagaaat ctactcttag gtaaatcaaa tctcactgtt tgtccacttg
      541 agtatatctt tgctggttgg tctactttat attctcttgt tgcttgtgca aataaaattg
      601 ccctttgtga tgctggtaac aaaacctttt tttctgccat aattaatatt tcctcacttt
      661 catattttaa ttatttttat actgcattta ctacatcttt aagtccttgt ggcactactg
      721 aatatttgtt aactaaaact tttgaaacaa ctatgaataa tactgccatc attcccacca
      781 ttaaaaaact tctcattcct aatttcattt tatcacctcc tttacaattt caatcttgct
      841 ttaattggta tttccatatg ttttacattg tagtaataaa agaagtattc ttcgcccaaa
      901 cttttatcat ttggattatt ttcaaatact gtacttccag tgttttcaac tatgcgtttt
      961 ctatcgtctt tagtgttaag gtcgaataca aaatagtgaa ttgcctcaga taaaaagacg
     1021 gtaggtattc ccttgggacg ttgagtgcat ccccacattt gggtgttgcg acttctacct
     1081 ctagttaaaa ttgcttttgc gtaaaatgga attgaaaaac tattaggaca aacgtctaaa
     1141 atttcatcaa ctagacacgt acaatttttt ctgaaatata tccattcaaa aaacaattca
     1201 taatatgttg aatttaattc ttgaatatta ggtctgtaaa ttgccttacc ttctttgaaa
     1261 gtttgtaatt cctccagtga ttcaaacact ggcacattag gtaactgtga atatttaaaa
     1321 tcaccttttg tgtcaagcac tattacatta tccataccag cagtatatat ttcagctagt
     1381 ttacttttac ctgttccact tctcccacaa tcaaaaacat gttcgtcttt gttaattact
     1441 atttgcaatt acatcagctt caatcacatt ttcattttca atttcaaata tgaattgttt
     1501 tgttattttg ataatttcct ctgttttttc atcatctaaa gatgaacaaa atttagccac
     1561 taatggcttt aataaattga ccatcactgg tgatactgct tgtgtcgcct ccgacattat
     1621 tttctgtatt gctaattgta gtcgaactgt tttctgtatc atctttcata cctccatttg
     1681 cttttataac tttcatttgt ttgttgctct tttgtgaatt taattgagat tgaattatta
     1741 ttctaggaat agcaatcgaa actgttgcaa ctactaatgc tacaccatct gaaacaccag
     1801 ctattttatc aactaaattg aatttattta aaatactatt tagtggtgtt gctatagaat
     1861 tactttcagc ttcatctatt gtccagtgtt ccccaaactt taaagatgca attacagtta
     1921 atgtttgaat caatgtagat atttcagcaa tggcatcaac ttttgtttct tcaactttca
     1981 catttttttt a
//
LOCUS       DASWBY010000676         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_140741, whole genome shotgun sequence.
ACCESSION   DASWBY010000676 DASWBY010000000
VERSION     DASWBY010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140741"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF870_12320"
     CDS             complement(<1..287)
                     /locus_tag="VF870_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9253023.1"
                     /translation="MEYYNNLSQQPPKQLKFEEIFAFYYNLKGASNADDGKFDEAIKN
                     FDKAIELNPELSSALFNRATIKADLGDLKGAREDFVRVREIELKHNTDLYQ"
     gene            667..>1501
                     /locus_tag="VF870_12325"
     CDS             667..>1501
                     /locus_tag="VF870_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9253024.1"
                     /translation="MSKQQSSKTGFKSFPLNYWVVIMMEFFERGSYYGMMSILSVYMT
                     DQLSYTKESVGVIKSVIQPMLYILPILSGAIGDRFGYKRVLTFAFIFLGLGYFLTSQT
                     TEYAMVFGSLIIMATGAGAFKPMISGTIARSTNEGNSTLGFGIFYWSINLGAFLFPLI
                     LVPYIKNTFGWEYVMMASAIATASMLIPTFLIYKEPKRPESTKTLAEVFKGAVMVLTD
                     FKFIGLIVIYSGFWILYFQMFDSVLWYVQKYVDATSLNNFVNGTLGGIGININWKFDV
                     EH"
BASE COUNT          457 a          232 c          258 g          554 t
ORIGIN      
        1 ttttgatata aatctgtatt atgttttaat tctatctctc ttacccttac aaaatcttct
       61 ctggctcctt taagatcgcc aagatcagct ttgatagtag cacggttaaa taaagctgaa
      121 cttaattcag gatttaactc aatagcttta tcaaaattct tgattgcttc atcaaactta
      181 ccatcatctg cattgcttgc cccttttaga ttataataga atgcgaatat ttcctcaaac
      241 ttaagttgtt ttggaggctg ctgggataag ttattgtagt attccatttt tggttccttt
      301 tgatttttgt ctcgctattt tataatagtt ttaattttct atcagatttt tcttacttgc
      361 aaatatattt ctgttagatt aacaccagat taatgaaaga ttaaaaaaaa ttaatgtggt
      421 atggttttca ctgaaggtaa taaatggaca actccaatat taaaatacat agtagaattt
      481 gtagctcagg tttccaaacc tgagcttttt tatataataa tacttttaaa tatctcagga
      541 ctggagttct gagctacaag gcatttaatt cggcacgagc aatcgtgcct ttttattata
      601 tcttaactca cattttctta gatttgcatt caataaataa aatttgaaaa ctaaaaagga
      661 cagcagatga gcaaacaaca atcatcaaaa acaggattca aatccttccc attgaattat
      721 tgggttgtaa taatgatgga gttttttgaa cgaggttcct actatgggat gatgagtatt
      781 ctttctgtgt atatgaccga tcaactcagc tacactaaag aaagtgtggg tgttattaaa
      841 agtgttattc agccgatgct ttatattctc ccaattcttt cgggtgcaat tggagaccgt
      901 tttggctata aaagagtatt aacttttgca tttatctttt tgggattggg atattttctt
      961 acaagccaaa ccacagaata tgcaatggta tttggcagct taataattat ggcaacagga
     1021 gctggcgcat tcaagccaat gatttcagga acaattgcaa gatcaacaaa tgaaggtaac
     1081 agtactcttg gttttggaat tttttattgg tcaataaatc taggtgcttt tttgttcccg
     1141 ctgattcttg ttccttatat taaaaatact tttggctggg aatatgttat gatggcatct
     1201 gcaattgcca ctgcttcaat gctaattcca actttcctta tatacaaaga acccaaaaga
     1261 cctgaaagca caaagacact tgcagaagtt tttaaaggtg ctgtaatggt tttaactgat
     1321 tttaaattta ttggattgat cgtaatctat tcgggtttct ggattttata ttttcagatg
     1381 tttgattcgg ttctatggta tgttcaaaaa tatgttgatg ctacatcgtt aaacaacttt
     1441 gttaacggta cacttggcgg aattggaatt aatattaatt ggaagtttga tgttgagcat
     1501 g
//
LOCUS       DASWBY010000677         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_592054, whole genome shotgun sequence.
ACCESSION   DASWBY010000677 DASWBY010000000
VERSION     DASWBY010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592054"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..>1667
                     /gene="acs"
                     /locus_tag="VF870_12330"
     CDS             77..>1667
                     /gene="acs"
                     /locus_tag="VF870_12330"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX9253025.1"
                     /translation="MAEKKLTGDVYYPTKEIMDYAHAKCESLYKSAEKDPIGFWEAEA
                     NNLHWFTKWDKVLDDSNKPFFKWFTGAKTNIAFNCLDVHVQTPRRNKLALIWEGEDGE
                     FRSYSYFKLHRETCRFANVLKSLGVKKGDRVTIYMGRVPELMIGMLACARIGAIHSVV
                     YGGFSVEALHERLEDSQSKVLIVADGAYQRGKIVELKKIADEALQRAATVESVLVVKR
                     TGHQINMEFGRDMWYHELVNLPIANSAECLEIVDAEDPLFMLYTSGTTGKPKAIMHTH
                     GGYMVGTYTTLKYVFDIHEEDRFWCAADPGWITGHSYIVYGPLLNGATSFMYEGAPTY
                     PYPHRWWQMIEKYGINILYTAPTAIRGLMRYGDAWPNRYNLSSLRLLGSVGEPINPEA
                     WKWYHRVIGKEKCPIMDTWWQTETGMFMITPMPCTPLKPGSGTKPFPGLIMDIVDEEG
                     NSVKPNEEGFLVIKTPWPSMLRTIWNDPDKYVNQYWSKYPGMYMTGDSARRDEDGYYW
                     VIGRVDDVIKVSGYRLGTAEIE"
BASE COUNT          556 a          263 c          366 g          482 t
ORIGIN      
        1 tattgattct catctaaact acttttaatt tcagccaggt ttatttataa cattctaatt
       61 ttttcaggag ttacaaatgg cagaaaaaaa actaaccggt gatgtttatt atccaacaaa
      121 ggaaataatg gattatgcgc atgcaaaatg tgaaagtttg tacaaatctg ctgaaaaaga
      181 tccaattgga ttttgggaag ccgaagcaaa taatcttcat tggtttacta aatgggacaa
      241 agtacttgat gattccaata agcctttctt taaatggttt accggggcaa aaacaaatat
      301 agcttttaac tgtcttgatg ttcatgttca aactccaaga agaaataagc ttgcattaat
      361 atgggaagga gaagatggtg agttcagatc ttattcttac tttaaactgc acagagaaac
      421 gtgccgcttt gcaaatgttt taaaaagtct tggagttaaa aaaggcgatc gtgttacaat
      481 ttatatgggt agagttcctg agctaatgat tggaatgtta gcctgtgcaa gaattggtgc
      541 aattcactca gtagtttatg gtggattttc agttgaggca ttgcacgaaa gattagaaga
      601 cagtcaatca aaagtattga ttgtcgcgga tggtgcatat caaagaggaa aaattgttga
      661 actaaaaaaa attgctgatg aagcattaca gcgtgctgca actgttgaaa gtgtattagt
      721 tgtaaaacgg acaggtcatc agataaatat ggaatttgga agagacatgt ggtatcacga
      781 acttgtaaat cttcctatag caaatagtgc tgaatgtctg gaaattgttg atgcagaaga
      841 tccattattt atgctttaca catccggcac aacaggaaaa cctaaagcta taatgcatac
      901 gcacggcggc tacatggtag gaacatatac cacgctaaaa tatgtattcg atattcatga
      961 agaagataga ttctggtgcg cagctgatcc tggctggatt actggccaca gctatatagt
     1021 ttacggtcca ttactgaacg gtgcaacatc atttatgtat gaaggtgctc caacctatcc
     1081 ttatccgcat cgctggtggc agatgattga aaagtatgga ataaatattt tgtacactgc
     1141 tccaacagct atacgcggtt tgatgagata tggggacgca tggcctaatc gttataactt
     1201 atcatcttta agattgttag gaagcgttgg tgaaccgatc aatccagaag cctggaaatg
     1261 gtatcatcgt gtaataggaa aagagaagtg tccaatcatg gatacctggt ggcaaaccga
     1321 gaccggaatg ttcatgataa ctccaatgcc gtgtactcct ttaaaaccgg gatcaggaac
     1381 aaaacccttc cctggattaa ttatggatat tgttgatgaa gaaggtaact ctgtaaaacc
     1441 aaatgaagaa ggttttcttg ttatcaaaac accctggcca tcaatgctca gaacgatatg
     1501 gaatgatcct gataaatatg taaatcaata ttggagtaaa tatcccggaa tgtatatgac
     1561 cggtgattct gcacgtagag acgaggatgg ttattattgg gtaataggaa gagttgacga
     1621 tgtaataaaa gtttccggtt atagattggg gacagcagaa attgaaa
//
LOCUS       DASWBY010000678         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_254789, whole genome shotgun sequence.
ACCESSION   DASWBY010000678 DASWBY010000000
VERSION     DASWBY010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254789"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VF870_12335"
     CDS             <1..1034
                     /locus_tag="VF870_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253026.1"
                     /translation="SSGSFVNDLVVDGKNIARNLLLSYPYYDPTSGQYFEDRTILNSN
                     LQQKGSLISDGGINNWSAGASYEFAHNVFFGASVNYTIGTLQSNREFIETDINDAYPL
                     TTLTIPDSNLTAGFNSYYLNDVVDWSYNGWDGRFGLLYKFFDFIRLGGSVKLPTKFVI
                     TEDHYFNGQSTFSTGYTKTLNVPVISSQYTITSPFEFTAAASVNLWFVTAAAEVSYTD
                     YTQMRFSGDEDPPVYAEINKQILEKYTQVFNLRAGAEFRLPFTGLSARAGFMYNPSPL
                     ADTPTEYDRKILNAGLGISSGEGDLEFNVTYSRSWWDEPGEGFGTNVPPVDQSIRIDN
                     IMTSFTVRF"
     gene            1174..>1755
                     /locus_tag="VF870_12340"
     CDS             1174..>1755
                     /locus_tag="VF870_12340"
                     /inference="COORDINATES: protein motif:HMM:NF022812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HEX9253027.1"
                     /translation="MKKTSVLIITILLAIFSISSFSQSNFNIEAYKQFLQSHQNLEPD
                     QLMQMHPAGNFIGDLNLSLSNTRYLDSISIKYGLTPHELELLEQNGFVVSERMKKISF
                     GEAFLEIFHKDLPVFVSTDAILHAFHISYDRILKDAELGFLIEKVKTLLQTLHSQQSY
                     LNSKYISNPGMMKMLKDVDVYLTVPLKLFELPVN"
BASE COUNT          551 a          285 c          304 g          615 t
ORIGIN      
        1 attccagcgg ttcatttgtt aatgatttag tggtagatgg aaagaatatt gccagaaatt
       61 tgttattaag ttatccttat tacgatccaa cttctggtca gtattttgaa gatagaacaa
      121 tccttaacag caatcttcag caaaaaggtt cgcttataag tgatggcgga attaataact
      181 ggtcagcagg cgcatcgtat gaatttgcac ataatgtttt ttttggtgca agtgtcaact
      241 atacaatcgg tactcttcaa agtaataggg aattcattga aactgatata aatgatgctt
      301 atcccttaac aactttgaca atcccggatt caaatttaac tgccggtttt aactcatact
      361 atcttaatga tgttgttgac tggagctata atggatggga tggaagattt ggtttacttt
      421 ataaattctt cgattttatt cgccttggcg gatctgtaaa actgcctact aagtttgtaa
      481 tcacagaaga tcattacttt aacggacaaa gtactttttc tactggttat acaaaaacgc
      541 tcaatgttcc ggtgatttca tctcaatata caattacatc gccatttgaa tttacggccg
      601 cagcttctgt taatttatgg tttgttactg ctgcagctga ggtatcctat actgattaca
      661 ctcaaatgag gttttcaggt gatgaggatc ctcctgttta cgccgaaata aataaacaaa
      721 tccttgaaaa atatactcag gtttttaatt tacgtgctgg tgctgaattc agacttccgt
      781 ttacaggatt gagtgcacgt gccggattta tgtataatcc ttcaccactt gctgatacac
      841 caactgaata tgatagaaaa atattaaatg ccggactcgg aattagttct ggtgaaggtg
      901 acttggaatt taatgtaact tattcccgca gctggtggga tgaacctgga gaaggtttcg
      961 gcacaaatgt tcctcctgtt gatcagtcaa ttcgtataga taatattatg acttcattta
     1021 cagtaaggtt ttgattagcc cagtttaaat tttttataaa taaattattt caaagtccca
     1081 tagttaattc tgtgggactt tttttatttc atattcgtta attgatattg tcttctaatt
     1141 ttcatacgtt tgataaaaaa atcggtgaat aaaatgaaaa agacatcagt tttaattatc
     1201 accattttac tggccatctt ttcaataagc tcttttagcc aatctaattt taacatagag
     1261 gcatataaac aatttctcca atcacatcag aatttagaac ccgatcagtt gatgcagatg
     1321 catccggcag gaaattttat tggtgatcta aatttatcat tatcaaatac aagatatctt
     1381 gattcaattt ctattaagta tggtttaact ccgcacgagt tggaattact ggaacaaaat
     1441 ggatttgtag tatctgaaag aatgaaaaag atttcttttg gagaagcatt tcttgagatc
     1501 tttcataaag atctgcccgt atttgtttca actgatgcta tacttcatgc atttcatatt
     1561 tcttatgata gaatattaaa agatgcagaa cttggattct taattgaaaa agtaaaaaca
     1621 cttttacaaa ctctccattc acaacagagc taccttaatt caaagtacat cagtaatcct
     1681 ggaatgatga agatgctgaa ggatgttgat gtttatttga ctgttccatt aaaattattt
     1741 gagttacctg taaat
//
LOCUS       DASWBY010000679         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_63943, whole genome shotgun sequence.
ACCESSION   DASWBY010000679 DASWBY010000000
VERSION     DASWBY010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63943"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VF870_12345"
     CDS             complement(<1..876)
                     /locus_tag="VF870_12345"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9253028.1"
                     /translation="MNITFHQLRIFLEVVKQKSITKSAEQMHMTQPALSIQLRNFQQQ
                     FDIPLIEIINKRLYITDFGYEIAELAKNVLNEADAIELKTKEYKGSIRGKLKISSAST
                     GKYVIPFFLNGFLKENNGVDLMLDVNNKTSTIRSLLNNEIDFALVSIIPDDIKVKEEL
                     LVDNGLYLVGATKNYDTEKPLIYREPGSATRTVMENYFAKIKKRKSIELTSNEAVKQA
                     VMADIGYSILPLIGIKNELLKKQLFIINAKGLPVTTKWRLIWLENKKLSPVARAYLDY
                     LSVNKEKIISNNFKWY"
     gene            963..2396
                     /locus_tag="VF870_12350"
     CDS             963..2396
                     /locus_tag="VF870_12350"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX9253029.1"
                     /translation="MQRITIAKGDGIGPEIMNATLEILKAAKARLEYEEIEVGEKVYL
                     SGNTSGIDKTAWDSIRKNKVFLKSPITTPQGSGYKSLNVTMRKALGLYANVRPCKSFY
                     PYVFSKHPEMDVVVIRENEEDLYAGIEHQQTDEVVQCLKLITRPGCEKIIRYAFEYAR
                     LYKKNKVSCFTKDNIMKHTDGLFHQVFKEISQEYPDIKADNWIIDIGAAKLATNPEMF
                     EVLVMPNLYGDVVSDIAAELSGSIGLAGSANIGNQCAMFEAIHGSAPTIAGLNKANPS
                     GLLRAAVMMLNHIGQQDVAELIKNSWSCVIEKGIHTEDIFNKDISKKLVGTAEFAKAV
                     IDHLGEKPQKLHSAEYSREVKLNIQPYVRKKESVKVLKGVDVFVDWKGKNPTELAEKL
                     QILNNKIQLKMITNRGVKVWPEGFEETFLTDHWRCRFEVENGRPASKAAIPDLMFQAL
                     LHHIDIIKTENLYEFDGKKGYSLGQGQ"
     gene            2620..>2825
                     /locus_tag="VF870_12355"
     CDS             2620..>2825
                     /locus_tag="VF870_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253030.1"
                     /translation="MNEETIIMDRPTGPEELKLVAESGYKKWPPRLSGQPFFYPVTNE
                     EYAKQIAMKWNLKSSGVGYVTKFKV"
BASE COUNT          953 a          438 c          538 g          896 t
ORIGIN      
        1 ataccattta aaattattac taataatttt ttctttgttg acggagagat aatctagata
       61 agcccttgca accggagaca gctttttgtt ttctaaccag attaatctcc attttgttgt
      121 gacgggtaag cctttagcat ttattataaa tagctgcttt tttaataatt catttttaat
      181 accaataagc ggtaaaattg aatacccaat atcagccata acagcctgct taactgcttc
      241 attcgaagtg agctcgatac tttttctctt tttgatttta gcaaaataat tttccatcac
      301 ggttcttgta gcggaacctg gttcacgata aataagaggt ttctccgtat cataattttt
      361 agttgctcct acaagataaa gcccgttatc aaccagtagt tcttctttaa cctttatatc
      421 atcaggtatt atagaaacaa gagcgaaatc aatttcatta tttagtaaac ttctaatggt
      481 tgatgttttg ttattaacat caagcattaa atcaacaccg ttattttctt taagaaatcc
      541 gttcaagaaa aatggaataa catattttcc tgttgatgcc gaggatattt ttaattttcc
      601 acgaattgac cccttatact cttttgtttt caattctatt gcatcagcct cattcaatac
      661 attttttgca agttcagcaa tttcataacc aaagtcagtt atgtaaagtc tcttattaat
      721 tatttcaatt aatggtatat caaattgctg ctgaaaattt ctcaattgaa ttgataaggc
      781 tggttgggtc atatgcattt gttctgcaga tttagtgata cttttttgtt ttacgacttc
      841 caggaatatt cgtaattgat gaaatgtaat gttcataaaa ataacttatg gattttataa
      901 atattataaa tataaatata taaataatat cctttatttt tgagtcaaaa aaagaggaag
      961 taatgcaaag aataacaata gcaaaaggcg atggtattgg tccagaaatc atgaatgcaa
     1021 ccctggaaat acttaaagca gcaaaagcca gattagaata tgaggaaatt gaggttggag
     1081 agaaagtata tttatccggc aatacaagcg gtatagacaa aactgcgtgg gatagtataa
     1141 gaaaaaacaa ggtgtttttg aaaagtccaa ttacaactcc gcagggcagt ggttataaaa
     1201 gtctaaatgt aactatgcgc aaagcactgg gattatatgc aaacgtcaga ccttgcaaaa
     1261 gtttttatcc atatgttttc agtaaacatc ctgagatgga cgttgttgta atccgggaaa
     1321 atgaagaaga tctttatgcg gggattgaac atcaacaaac tgatgaagtg gttcaatgtc
     1381 ttaaattaat tactcgtcct ggctgtgaga aaataataag atatgcattt gaatatgctc
     1441 ggctgtacaa aaaaaataaa gtcagttgtt ttacaaaaga caacataatg aagcatacgg
     1501 atggtctgtt tcatcaggta tttaaggaaa tatcacagga atatcctgat attaaagctg
     1561 acaactggat aatagatatt ggtgctgcaa agctggctac taatccggaa atgtttgaag
     1621 tattagtaat gccaaatctt tacggagatg tcgtttctga catagctgct gaattatcgg
     1681 gatcaatcgg tttagctgga tctgcaaata ttgggaacca atgtgctatg ttcgaagcaa
     1741 ttcatggctc tgcaccaacc attgcaggac ttaataaagc aaacccctcg gggcttctaa
     1801 gggcagcagt gatgatgttg aatcatatcg gacaacagga tgttgctgaa cttataaaaa
     1861 attcctggtc ctgtgttata gaaaaaggaa ttcatactga agatattttt aataaagata
     1921 ttagtaaaaa gttagttggt actgctgagt ttgctaaagc tgtaattgat catcttggag
     1981 agaaacctca aaaactccat tcggctgagt actccagaga agtaaaacta aatattcagc
     2041 cttatgtcag aaaaaaagaa tctgtaaaag tactgaaagg tgttgatgtt tttgttgatt
     2101 ggaagggtaa aaacccaact gaacttgcag aaaaacttca aatattgaat aataaaattc
     2161 aacttaaaat gattaccaat agaggtgtta aggtttggcc ggaaggattt gaagaaacct
     2221 tcctaacaga tcattggcgc tgccgttttg aagttgaaaa tgggcgcccc gcatccaagg
     2281 cggctattcc cgatcttatg tttcaggcac tgctacatca tattgatata attaaaactg
     2341 agaaccttta tgaatttgat ggtaagaaag gttattcatt gggacaggga cagtagaggc
     2401 gtaaatttta ttaaagcttt cactaacaca gtgtgggcgt gttagctgct gaacttttag
     2461 atttaaaaac cgcagaataa aacttctgcg gttttctgtt ttaaaccttt gttttcttcg
     2521 ggaagaattg ggaagtttga taggtatggt ttaccgacta ttggctttgt cagaatcttc
     2581 agtaacaata attcaaaact agataagtaa cagtgctgga tgaatgaaga aactattata
     2641 atggaccgcc ccactggtcc tgaagaattg aagctggtgg ctgaaagtgg ttataaaaaa
     2701 tggccgcctc gattatcagg gcagcctttt ttctatccgg taacaaatga agagtatgca
     2761 aaacaaattg ccatgaaatg gaatttaaaa tccagtggag ttggatacgt aacaaaattt
     2821 aaagt
//
LOCUS       DASWBY010000680         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_254881, whole genome shotgun sequence.
ACCESSION   DASWBY010000680 DASWBY010000000
VERSION     DASWBY010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254881"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1152)
                     /locus_tag="VF870_12360"
     CDS             complement(16..1152)
                     /locus_tag="VF870_12360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9253031.1"
                     /translation="MIENILIVVFFILLIHYIIFLSGIYVGLNRLSKEENQQIPEESV
                     SIIVPFRNEAKNILRTYLNLTTQNFPPEKFEIIFVNDSSEDNSLQLLENLPKKKNVTI
                     YSVPHDYSVNAHKKRAIRFGIEKSKSDIIVTTDADCFHNKEWLTNLLKYMDNETGFVS
                     GPVEFISNNNLFSQMQKLEFAGLVITGAGLIGYGNPIICNAANIAYRKNAFEEVGGFT
                     YQMKLSSGDDELLMQKIHRDTDYKIKFALDKEAMVSTEPNPTIKDFYRQRKRWASKGL
                     FYGDHLLLLKLVLIFLFYLSLIIQPILGLFSSSIFFLTFLILFLIKLSTEYLVLKKGT
                     NLLFDSKILKPFLITEILQVPYILVSGFMGMFGNLVWKNRKISR"
     gene            complement(1145..2137)
                     /locus_tag="VF870_12365"
     CDS             complement(1145..2137)
                     /locus_tag="VF870_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX9253032.1"
                     /translation="MTAEEIKKSFDQKNFVSKYKTVIIVSAKVIIAFGLLIYLIYAVN
                     FQEILEAIKQANIYLISVAFLLSVVNIYLQFYKWKITANVVLQENHNAKIWLSLFYGF
                     SAGVFTPARIGEYFGRAIVFKNQSLLRVTLATLLDKLFLLLIVAFFGSISSILFVHYY
                     YHITFYLTIALFIIIFSLFYLFFWMIFNDRFWDNVFFAKLRDSIKLHWLFEKIKIFRK
                     LDKIYATKMFVVSFLFYLCFLVQYALSVAAFSNHNNYWDYLWAGNLMMFAKTIIPPVS
                     LGELGIREGASVFFITQFRESASTGFNASIFLFFINVLIPSLIGLILLLKKNDD"
     gene            complement(2141..>3122)
                     /locus_tag="VF870_12370"
     CDS             complement(2141..>3122)
                     /locus_tag="VF870_12370"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9253033.1"
                     /translation="RVLNTVKIISSFTLSRIVRKPIVWGMPISYSIEPTNHCNLKCPE
                     CPSGLGALTRPLGLLNFADFKNWIDQIKDTGFYVQLFFQGEPYINKHLPEMIRYAQQN
                     NVYISISTNGHFVNEKNVDQVLENAPDKLIYSVDGLDEESYQKYRVGGTFKQADEGLR
                     LLINRKKQLKKNKPYVEFQFIVMKQNEHQLDNVRKYCDEVGVDKLVFKTMQVSSYENA
                     IKFLPDNPKYRRYLVKDGKFSIKNKLKNHCFALWRTSVITWDGKVVPCCFDKDAEYTL
                     GILNGKRFSDIWISDEYQKFRSAVLVDRKQTNMCTNCTEGLKINILKIGQ"
BASE COUNT         1123 a          530 c          430 g         1039 t
ORIGIN      
        1 agatttcaaa aaagattagc gactgatttt tctatttttc caaacgagat taccaaacat
       61 tcccataaaa ccggaaacta aaatatatgg aacctgtaga atttctgtta tcaaaaaagg
      121 ttttaaaatc tttgaatcga ataatagatt tgttcctttt ttaagaacca gatattctgt
      181 agataattta attaaaaata aaattaaaaa tgttaagaag aatatcgaag atgaaaacaa
      241 tccaagaatt ggctggataa ttaatgaaag ataaaataaa aatataagaa caagtttcag
      301 cagtaaaaga tgatcgccat aaaacaaacc tttgcttgcc catcgtttgc gttgacggta
      361 aaagtcttta atagttggat ttggctccgt tgaaaccatt gcttctttat ctaaagcaaa
      421 ttttatttta taatcagtat cacgatgaat tttttgcatt aataattcat catcaccgga
      481 agaaagcttc atttgataag taaatccacc cacctcctca aaggcatttt tgcgatatgc
      541 aatatttgca gcattacata ttattggatt tccgtatcca attaaaccag cacctgtaat
      601 aaccaatcca gcaaattcta atttttgcat ttgactgaac agattattgt ttgaaataaa
      661 ttcaacggga ccagaaacga aaccagtctc attatccata tattttaaaa ggttcgttaa
      721 ccattcttta ttatgaaaac aatctgcatc tgttgtaaca attatatcgc ttttcgattt
      781 ttcgattcca aatcttattg ctcttttctt gtgtgcattt acagaataat cgtgtggaac
      841 tgaataaata gtgacatttt ttttcttcgg cagattttcc aataattgta atgaattatc
      901 ctcagaagaa tcattaacaa atattatttc aaacttttcc ggtggaaaat tctgagtggt
      961 aagattcaaa tatgttcgta aaatattttt tgcttcattt ctaaatggaa caattataga
     1021 aacagattcc tcgggtattt gttgattctc ttctttagat agtctgttca atccaacata
     1081 aatcccggat aaaaatatta tgtagtggat taggaggata aaaaaaacaa cgattaaaat
     1141 attttcaatc atcattcttt ttaagaagta gtattaatcc aatcaaagaa ggaattaata
     1201 cattgataaa gaataaaaag attgaagcat taaatcctgt cgaagcagat tccctaaact
     1261 gtgtaataaa aaaaacagat gccccttctc taattccaag ttcaccgaga gacactggtg
     1321 gaataattgt ttttgcgaac atcatcagat tgccggccca taaataatcc caataattgt
     1381 tatgatttga aaatgcagca accgataatg catattgaac aagaaagcac aaataaaata
     1441 agaacgaaac aacaaacatt tttgtcgcat aaattttatc aagctttcta aaaattttta
     1501 ttttctcaaa caaccagtga agtttgatag agtctcttaa ttttgcgaag aaaacattat
     1561 cccaaaatct atcattgaag atcatccaaa aaaataaata aaataatgag aatataatga
     1621 tgaataaagc gatcgttaaa taaaatgtta tgtgataata ataatgaacg aacagtatgc
     1681 ttgatatcga accgaaaaaa gctactatta acaaaaggaa taatttgtct aataatgttg
     1741 caagtgtaac tcgcaataaa gattgatttt taaaaactat agcacgacca aaatactcgc
     1801 caatcctagc aggtgtaaaa acgccagccg aaaatccata aaataaagaa agccaaatct
     1861 ttgcgttatg attttcctgc agcactacat ttgcagtaat tttccattta taaaattgca
     1921 agtatatgtt taccacactc aatagaaagg ccacagagat caagtatata ttggcctgtt
     1981 ttattgcttc taatatttcc tgaaagttaa ctgcatatat tagatagatt aacaaaccga
     2041 aagcaattat aacttttgct gaaactataa ttacagtttt atatttacta acaaaatttt
     2101 tttgatcaaa acttttttta atttcttctg cagtcacttt ttactgccca attttcagaa
     2161 tattaatctt taatccttca gtacaatttg tgcacatatt cgtttgtttt ctatctacaa
     2221 gcactgcgct tctaaatttc tgatactcat cagatatcca aatatcggaa aatcttttac
     2281 catttaaaat cccgagtgta tattcagcat ctttatcaaa gcagcaaggc acaacctttc
     2341 catcccaggt aattacagat gttctccaca atgcaaagca atggttcttc aatttattct
     2401 ttatagaaaa ttttccatcc ttaactaaat atcttctata cttcggatta tcaggaagaa
     2461 acttaatcgc attctcatag gaagaaacct gcatcgtttt gaaaacaagt ttatcaacac
     2521 caacttcatc acaatatttt ctaacattat caagctgatg ctcattttgt ttcattacaa
     2581 taaactggaa ctcaacataa ggtttattct tttttaattg tttcttacga tttattaata
     2641 gcctgagtcc ttcatctgct tgtttaaagg tgccgcccac acggtacttc tgataacttt
     2701 cttcatcaag tccatcaacg gaataaatca gtttatcggg agcattttct aaaacttgat
     2761 cgacattttt ctcatttaca aaatgtccat ttgtgctgat tgaaatgtag acattattct
     2821 gctgtgcata ccttatcatt tccggaagat gtttgttaat gtaaggctcc ccctgaaaaa
     2881 atagttgaac gtaaaatccg gtatccttta tctgatcaat ccagttttta aaatcagcaa
     2941 aatttaataa cccaagagga cgagttaatg ctcccaaacc ggatggacat tcaggacatt
     3001 ttagattgca atgatttgtt ggttcaattg aataagatat tggcataccc caaacaattg
     3061 gtttcctaac aatcctggat aaggtaaaag atgaaattat tttaacagtg tttaaaacac
     3121 gt
//
LOCUS       DASWBY010000681         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_141203, whole genome shotgun sequence.
ACCESSION   DASWBY010000681 DASWBY010000000
VERSION     DASWBY010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141203"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VF870_12375"
     CDS             <1..805
                     /locus_tag="VF870_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9253034.1"
                     /translation="MMENPYYLNESNGWLPDIDDIKSKINDKTKAIVLINPNNPTGSL
                     YTKENLQQIVDLALEHNLVIFADEIYDKLLFDGKKHISIASLNKDVSCITFGGLSKNY
                     MVPGFRIGWGIVSGRKEVLSDFIEAVNKILRARLSANHPEQYGIKPCLEGNHDHLVEG
                     MKKLTRRRDLTVEMLNAIPGISCVKPEGAFYAFPRLEMKQPDAHFVGELIRETGVVIV
                     PGSGFGQVPGTHHFRVVFLPNEQILEKAYKAIGEFFVKYQEKYPDLVEV"
     gene            999..2249
                     /locus_tag="VF870_12380"
     CDS             999..2249
                     /locus_tag="VF870_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9253035.1"
                     /translation="MDNCKVTTLSNGVKVVSEFIPYLKSFSLGFWFNIGARDENLRNN
                     GISHFIEHMLFKGTKTRSAKQISDEIESYGGYLNAFTSKEQTCYYGRGLSENFKKTFV
                     VLSDMIQNPSFKDSHIKKEAGVVIDELKDIDDNPEELIFDKFEESIFNGHSLSYPIIG
                     TEKNILSYNSNILHQFHKANYRLDNLLISVSGAIHHEEAVKLAEKYFIGKRTISNQKR
                     KNFVKQTVNHVVIEKEIQQVHSIIGRTTYGFNDPRRHKLKVLSTLLGDGSSSRLFQAV
                     REKLGMTYQINTFLNSYLDVSAFGVYYSTNDKQAGKVNEIVHREFTKLRNVEVKAKEL
                     KRVKEYIKGNTLMSLESTTNRMIRLANSLLHYGKIVPIEDILNKIDSVTADDIQKLSN
                     DVLNESTLSKIIICSKDNSIKKAA"
     gene            2277..>2482
                     /locus_tag="VF870_12385"
     CDS             2277..>2482
                     /locus_tag="VF870_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9253036.1"
                     /translation="MPKFNIDGKEIEFKQGQTIIEAARDYGIEIPHFCWHPKLSVSGN
                     CRMCLVEVEKMPKLVIACSTNAAEG"
BASE COUNT          871 a          367 c          445 g          799 t
ORIGIN      
        1 gatgatggaa aatccttatt accttaatga atcaaatggc tggctgccgg atatcgatga
       61 tataaaatct aaaattaatg ataagacaaa agcaattgtt cttatcaatc caaataatcc
      121 tacaggatct ctttatacaa aagaaaatct tcagcagatt gttgatttag ctcttgagca
      181 taatcttgta atctttgctg atgaaattta tgataagctt ttgtttgatg gaaagaaaca
      241 catttcaatt gcatcattaa acaaagatgt ttcctgcatt acgtttggcg gactttctaa
      301 aaattatatg gttcccggtt ttaggattgg ctggggaatt gtcagcggaa gaaaagaagt
      361 tctttctgat tttattgaag cagtaaataa aattcttcgt gcaagattat ctgcaaatca
      421 tccggaacaa tacggaatta aaccttgcct tgaaggtaat catgatcatc ttgttgaagg
      481 aatgaaaaaa cttaccagac gaagagattt aacagttgaa atgttaaatg caattccggg
      541 aatttcctgc gttaaacccg aaggtgcttt ttatgctttc ccaaggttgg aaatgaaaca
      601 accggatgct cattttgtcg gtgagctgat tcgcgaaacc ggtgttgtaa ttgttcccgg
      661 cagtggattc ggacaagtcc ccggtactca tcattttaga gtagtgtttt taccaaacga
      721 acagattctt gaaaaagcat ataaagcaat cggtgagttt tttgttaagt atcaggaaaa
      781 atatcctgat ttggtagaag tataaatttt agaaaatgtt taagtcattt cgaacggaac
      841 gggaaatctg ttaatagact ttgaaagatt tttcactgac tgaagtcggg ttcgaaatga
      901 cttttctatt ttaatttcga taatttcttt tcttgattga ctaaacctct ttatctatat
      961 ttgaacctca tttaattcat tattaataga taaaaattgt ggataattgt aaagtaacga
     1021 cgctctcaaa tggtgtaaaa gttgtttcgg aattcatccc ttatttaaaa tcattttcac
     1081 tgggattttg gtttaatatt ggtgcgcgtg atgaaaattt acgcaacaat ggaatctcgc
     1141 attttataga acatatgctg tttaagggga ccaaaactag atcagcaaaa caaatctcag
     1201 atgaaattga atcctacggc ggttacttaa atgctttcac ttctaaagaa caaacttgct
     1261 actatggcag aggtttatcc gaaaacttta agaaaacttt tgtcgtgctc tcagatatga
     1321 ttcaaaatcc ttctttcaaa gattcgcata tcaaaaaaga agcgggtgtt gtaattgatg
     1381 agttgaaaga tattgatgat aatcccgaag aattgatatt tgataaattt gaagaatcta
     1441 tttttaatgg tcacagttta agttatccga ttatcggaac cgaaaaaaat atcctttcct
     1501 acaattcgaa tattcttcat cagtttcata aagcaaatta tcgcttggat aatttattga
     1561 tctctgtttc aggtgcaatt catcatgaag aagctgtcaa acttgcggaa aaatatttta
     1621 ttggtaagcg aacaatatca aaccaaaaaa gaaaaaactt cgttaaacaa acagttaatc
     1681 atgttgttat tgaaaaagaa attcagcagg ttcattccat cattggaaga acaacctatg
     1741 gatttaatga tccaagaaga cataagttaa aagttttgtc tactttactt ggtgatggaa
     1801 gctcttcaag attgtttcaa gcagttagag aaaaacttgg aatgacttat cagataaata
     1861 cttttttaaa ttcttattta gatgtttctg cgttcggagt ttattattca acaaacgata
     1921 aacaggcagg aaaagtaaac gaaatagtgc atcgtgaatt tactaagttg agaaatgttg
     1981 aagttaaagc aaaagaatta aaacgcgtta aagaatacat caaaggcaac actttaatga
     2041 gccttgaaag tactactaac agaatgatac gattagcaaa ttcacttttg cattatggta
     2101 agatcgttcc gattgaagat atacttaata aaattgattc agttaccgca gatgatattc
     2161 aaaaattatc aaacgatgtt ttaaatgaat caacactcag caaaataatt atttgttcaa
     2221 aagataattc aataaaaaaa gcagcttaag tttaagatta aaatccagag tagattatgc
     2281 ccaaatttaa tattgatgga aaagaaatag agtttaaaca gggtcaaacc atcattgaag
     2341 ccgcacgtga ttatggaatt gaaattcctc acttttgctg gcatcctaaa ttatctgtat
     2401 cgggtaattg cagaatgtgt ttggttgaag ttgaaaagat gccaaaactt gttatcgctt
     2461 gttcaactaa tgccgcagaa gg
//
LOCUS       DASWBY010000682        10420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_64193, whole genome shotgun sequence.
ACCESSION   DASWBY010000682 DASWBY010000000
VERSION     DASWBY010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64193"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VF870_12390"
     CDS             complement(<1..216)
                     /locus_tag="VF870_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253037.1"
                     /translation="MSNQNVSRVSYSTSVIFHLILLLLFLIINLSYDYSSKEYVELSF
                     GVLGGAGSSGTEGTAFDEVLEKAEPQEK"
     gene            complement(224..628)
                     /locus_tag="VF870_12395"
     CDS             complement(224..628)
                     /locus_tag="VF870_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9253038.1"
                     /translation="MKFKLSKEPLSIFAYSSLTDIVMLLLIFFLLTSQFVIQTGVKVK
                     LPGSKMNEKSEPSRMVVTLTPMGGVFAGEEEISIDQLPVKLGEMKAQTNEDNLIIRAD
                     KTVAIDLVIKVIDAAKISNIEKFTIETEKEKL"
     gene            complement(637..1293)
                     /locus_tag="VF870_12400"
     CDS             complement(637..1293)
                     /locus_tag="VF870_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9253039.1"
                     /translation="MNLLDIFLKGGFIMWPILLSSIIGLAVSIDRFIMLRKAKINVPA
                     FMVRIRGFIKKKDISGAISYCMQEKSPVANIVRKGLKKYKYGHDRVKDAIENAGSQEV
                     SKLEKGLSVLASVAGIAPLLGFLGTVTGMIQAFMTIQDLAGAANPSDLAGGIWEALIT
                     TAFGLIIGIPAFALYNYFLSAVKKLVGEMETVANDVIDVIQDGGKDEVEIDDEIEMEL
                     "
     gene            complement(1315..3369)
                     /locus_tag="VF870_12405"
     CDS             complement(1315..3369)
                     /locus_tag="VF870_12405"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX9253040.1"
                     /translation="MTKAELIRKVAKNVGVPDTDAKIFFELFLKRISAVVAIGQSIFV
                     KDFGYFHLIKGSIKKPVLSFNDNENLEEAVEFVLFSEDKELRKSDTKGLVFNIPFFDE
                     DDYHPIDSAFSLSIGKPLIPLRGVPFDNIYMPASGYEYRRLIESKVEKIIAASEIAES
                     EENFPTLVIDASSYNSNQVQLKWDDKSVIDKTDKHSVEDEIETQVKPELSQYEKQAKE
                     LKNIAWDFGEDLSKQIEVESILDIADEHINLDTSLKQKAEKNDDSEKIENTIEKVNLI
                     QNDSPSEKELNFETESVEEIILDNSSEKLDELLESDNDSNGIYDEEILQENAADNYQD
                     DIEEIIIEDLTEDTVKEEKSESVENASVEDEISDEEFWKSTSKYFEPYKPGTEKNNYE
                     KIEEVKSDSENLGVVDSEETSQDNDKEIRDVIQNEVVIKDHRVLEENVKETENTGQNN
                     ILNKNISKSKIELEKVDDQKDEYFETAKKKNLIPFIVFPLLVIILSLALYWYLEFYKK
                     SEVTPKVTQIALKTDNAKIVQRNFDIPITYPYLPKVNETSISETVVPKNEDQNSAVTK
                     NEAVNTEPVQIGIKKAEKEISKQNVTETKQPVKPVNPTGNSLNVGNNIYKYGNYFVVQ
                     VAAFRSSSISENEAGKYRNKGYNAFVEAAEIPGRGTWYRVRVGNFNTKEEALIFAKKN
                     IR"
     gene            complement(3387..4982)
                     /locus_tag="VF870_12410"
     CDS             complement(3387..4982)
                     /locus_tag="VF870_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9253041.1"
                     /translation="MRLKILLILSFSILAVYSQEDKTKNPNVELPDFVITGKSQLNIK
                     RVDKIKPDFVSSVNEDFVKPTYSPEELEIGDFSNPLKSDMSFLDDVSFYKGNIAVGIG
                     LYTIPTVSATYAHPFTNGIIEGIAKGNFTRAYEDNSDRFSTRFGFNFTYWSDIDGEIL
                     PGTQFSVNGDYGTTAYKFFASDNPTKKRTLNYGKFVVDVKNDFGKNFLFGLNLTNNVT
                     NISEEEFDENNLRIKGEALLKLSAVNIGAAVDYRNHSIKNLLGDNSGKDFFLLRPTAG
                     LFFTKLIKGSVGWTFSHGAGETYNALYASVAIKLDKNITLFGEYAPTAEFMSPGSFVV
                     KNNYFKVDSIGSIYWEKSNALTASVKYEFDKYFQIDGGLKYFTSDAFPYFGPSSDSGK
                     FNLHYADMKNISPYANFLFYLGPSGELYSSVELSDIHDNDGNQIPYMPAFKLNAAYTY
                     KFQNRLSTTARLDYLSNRYADIQNNISLGNYFDLGLSFIYSFQPNLDLTLDLNNVLNQ
                     KNYYWNGYKEVPLNIIIGINYRL"
     gene            complement(4979..7951)
                     /locus_tag="VF870_12415"
     CDS             complement(4979..7951)
                     /locus_tag="VF870_12415"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9253042.1"
                     /translation="MLKFFSLIILITIISPVYSQTSSQKYDEAMTAYHNQQYAQSNRM
                     FEDFFNEYKIVDEVYATAKYYSAESLLKLGRKDEAAIGFEYLVKTYNWSNYRDKALYN
                     LGLIYYSSGKYELSRNKLKLLLNEYPESDYYGNSLYWVGESFYIENKPDEAIQFLEEA
                     VADKRNKKFADYSIYALANAYEQTGDYTNAVKYYDQLLTYHKNSTLATSAQIRIGICY
                     FKLKDYQSSIVELKSPRLTNLSDDMYSESIYLLATSYYRVGEYSDAEKSYLEVLQKFP
                     DAKYARNAKYGLAWTYFQQKKYNQAFTEFDNLSTESDTIGIKSLFWKGECKRYLGKNN
                     EAMAIYKEFLEKYPDEPLAQEVQYQLGVVYFYNDKTDLASRYLISANSSDDQIIKSKS
                     FAMLGEIELNKGNFKAAKNYFDIVISNQGAEKSVHSRALLGLGISLYHLNDNKTALNY
                     LNELENSDPGFETQKTNFFLAENYFATGDYKNALNNYNASSGADQEINSQTLYGKAYS
                     YFNLGDYENAAYQFADYIKKYPKNKRLSDAKLRLADSYFGSKNYAAASKVYQELFSAD
                     AKSSTDPYARYQYAQALYKSGKTDEAINEFKLIQEKFPNSEYAEVSLYTIGWIHFQKK
                     EYAEAIDQYNNVISKYPKTKLEPIIYYSIGDAQFNMEKYDLAIENYEKVISQFPSSQY
                     VFDAVTGIQYSYVAKGNPEKAVSFLDGFVSKNPSLSYADQLMFKKGEIYYSLRQYDKA
                     KTEYMSFTSAYPNSKLVPDGYYWIGKSAQNLGQNEEAIFNFEKVFDVYKNSELASASV
                     LEMGLIYRSLKNYDAAISIYDRGINELKKSAKIPEFQYNKGMTLLEKNNFQEAYSVFE
                     EVVMYYPGTVFGDKSKFELALIDIAAKRYSSADEYLTALSENRTDDLGAKSQYYLGQS
                     LYDQGKYNEAITSLVRVRTVYSRYEEWLMRSYLLLGDCYVKLNDKRNAAEMYRAVIAK
                     YSGTPIGNEAQQKLRKVQ"
     gene            complement(8211..8855)
                     /locus_tag="VF870_12420"
     CDS             complement(8211..8855)
                     /locus_tag="VF870_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253043.1"
                     /translation="MKLDKLSELELLALLSASSVLRKEKYFDSILEVLLIKKYSVKKI
                     YEALLQTYLFAGFPSALISLKRLSEIAGKDKVYKGYDLKKYLSRGEMNCRIIYGNKYD
                     KLISNVKSFSPEMAEWLIVEGYGKVLGREGLTLKDREVCIVSILSALKFKDQLYSHIN
                     GAVQVGTDFNEILKTIKNLELISAKPSVKFGLCVFEEYQIKKTKNTSKSDSEIS"
     gene            complement(8920..9681)
                     /locus_tag="VF870_12425"
     CDS             complement(8920..9681)
                     /locus_tag="VF870_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9253044.1"
                     /translation="MTKNGLLLSVLLLLSISILGFAQEMKPEAGKLYNEGNALLKEGN
                     ANDAIEKYNAALAIEKDYRIYYQKGVALKKANKLEDAKVAFEECLKLNNGYEGALNAL
                     GGVYFSMGNYQAAIDNFQKVLNSNASNSIKTKVKKNISLAYAKLGNESLAGGDANKGI
                     EYLKKSVENDNYDAAYLSLAKVYSETGKYDEAIEAAQNALKYRSNITKGGPYYYIGIA
                     YKYKGDTAKAKDNFKLAAADPTYKKTADYELSLIK"
     gene            complement(9723..9806)
                     /locus_tag="VF870_12430"
     tRNA            complement(9723..9806)
                     /locus_tag="VF870_12430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9770..9772),aa:Leu,seq:gag)
     gene            complement(9877..10347)
                     /locus_tag="VF870_12435"
     CDS             complement(9877..10347)
                     /locus_tag="VF870_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253045.1"
                     /translation="MYKLCMMVVAVVLLNSISFSQEMRERGMKNRDKLEQLEKIKLIE
                     TLDLDEETSIRFFARRNDSRGEIQDLERKADDIIFELEKSFNSSDKNQDEKYKKLISE
                     MLKIRESIESKRNQFIKSLDDILTKEQIAKLIVFEKKFRDEIRNVLFDRKPRNN"
BASE COUNT         3279 a         1907 c         1463 g         3771 t
ORIGIN      
        1 tttttcctgt ggttctgctt tttctaatac ttcatcgaac gcagttcctt ccgtacctga
       61 tgaaccagca ccgcctaaaa cgccaaatga taattcaaca tactctttag aagaataatc
      121 ataagacaga ttgattatca ggaacagtaa aagtaaaata agatgaaata ttactgaagt
      181 tgagtatgag acacggctaa cgttttgatt tgacaaatac atcttaaagc ttctcttttt
      241 cagtttctat tgtaaatttt tctatgtttg aaatttttgc tgcgtcgata actttaatta
      301 ccagatcaat tgcaacagtt ttatcagcac gaattataag gttatcttca tttgtttgag
      361 ctttcatttc accaagctta acaggaagtt gatcgatgct tatttcttct tcacctgcaa
      421 aaacgccgcc catcggtgtt aaggttacta ccattcttga gggttcggat ttttcattca
      481 tctttgaacc gggcagtttt acttttactc ccgtttgtat aacaaattgt gatgtaagca
      541 aaaagaatat aagcagcagc atcacaatat ctgttaaaga ggaataagca aatatgctta
      601 atggttcttt tgaaagttta aatttcattt tgttacctat aattccattt caatttcatc
      661 atcaatttca acttcatctt ttccgccatc ctgaattaca tcaatcacat catttgcaac
      721 tgtttccatc tcaccgacta attttttgac ggcacttaag aaatagttat ataatgcaaa
      781 tgcaggaata ccaattatca aaccaaacgc tgtggtaatt aatgcttccc aaataccgcc
      841 ggcaagatca cttggatttg cagcacctgc taaatcctga atggtcataa atgcctggat
      901 cattcctgtt acagttccaa gaaatcctaa taaaggtgcg atacctgcaa cagatgcaag
      961 aacagataat cctttttcca gtttactaac ttcctggctc ccggcatttt caattgcatc
     1021 tttaactcta tcgtgaccgt atttgtattt ctttaaacct ttacgaacaa tatttgcaac
     1081 aggtgatttt tcctgcatgc agtagctaat ggcaccggaa atatctttct tttttataaa
     1141 tcctcttatt ctaaccataa aggctggaac attaattttt gcttttctta acattataaa
     1201 ccgatcgatc gatactgcaa gtccgattat cgagctgaga agtatcggcc acataataaa
     1261 tccacctttt aagaaaatat ccagtaagtt catattcatc ctttaataat ttaattatct
     1321 aatatttttt tttgcaaaaa ttagtgcttc ttcttttgta ttaaaatttc ctactctaac
     1381 tctataccag gtaccgcgcc cgggtatttc agcagcttct acgaatgcat tgtatccttt
     1441 gttcctgtat tttccagctt cattttctga tattgaactc gatctgaaag cggcgacctg
     1501 cactacaaag taattaccat atttataaat attgtttcca acgtttaaag aatttccagt
     1561 cgggtttaca ggtttaacag gctgcttagt ctcagtcacg ttttgttttg aaatttcctt
     1621 ttcagctttc ttgatcccaa tttgaactgg ctctgtatta actgcttcgt tttttgtgac
     1681 tgctgaattt tgatcttcat tttttggaac aaccgtttca gaaattgaag tttcattaac
     1741 cttagggagg taaggatatg ttattggaat atcaaaattt cgctgaacaa ttttggcatt
     1801 atctgttttt aaagcaatct gagttacttt tggtgtaacc tcactttttt tataaaactc
     1861 taaataccaa tacagagcta aagataaaat tattacaaga agtggaaata caataaaggg
     1921 tattaaattt ttcttttttg ccgtttcaaa atactcatct ttttgatcat caactttttc
     1981 taattcaatt ttactttttg aaatattttt gttaagaata ttattttggc ctgtattttc
     2041 ggtttcttta acattttctt ccaaaacacg atgatccttt ataacaactt cattttgaat
     2101 tacatctcta atttctttgt cattgtcctg gctagtttct tcagaatcaa ccacgccaag
     2161 attttcggaa tctgatttta cttcctcaat cttttcatag ttattttttt cagtaccggg
     2221 tttgtaaggt tcaaaatatt ttgaggtgct tttccagaat tcttcatcag atatttcatc
     2281 ttccacagaa gcattctcaa ccgattctga tttttcttct ttaacagtgt cctctgttaa
     2341 atcttcaatt attatttctt caatatcatc ctgatagtta tcagcagcgt tctcttgcaa
     2401 tatctcttca tcatagattc cattagaatc attatcgctc tctaatagct catccaactt
     2461 ttctgatgaa ttatccaaaa taatttcttc aacagattct gtttcaaaat tcaattcttt
     2521 ttctgatggt gaatcgtttt gtatcaaatt cactttttca attgtatttt caattttttc
     2581 ggagtcatca tttttctctg ctttctgctt taaagaagta tccaaattga tatgctcatc
     2641 tgcaatatca agaatcgatt cgacctcaat ttgcttagaa agatcttcac caaaatccca
     2701 tgcaatattc tttaattcct tagcttgttt ttcatactgg ctcaattcag gttttacctg
     2761 agtttctatt tcatcttcta ctgaatgctt atccgttttg tcaattacag atttatcatc
     2821 ccactttaac tgaacctgat ttgaattgta tgagctagca tcaattacaa gagttggaaa
     2881 gttttcttca gattctgcaa tctctgatgc agctataatt ttttctactt tggattcaat
     2941 taatcttctg tactcgtatc ctgaagcggg catgtaaatg ttatcaaagg gaacaccgcg
     3001 taaaggaatt aaaggtttac caatacttaa actgaaggct gaatcgattg gatgataatc
     3061 gtcctcatca aaaaaaggaa tattgaaaac aagtcctttt gtatcagatt tcctcaattc
     3121 tttgtcttct gaaaaaagta caaattcaac tgcctcttcc aggttttcat tatcgttaaa
     3181 acttaaaacc ggttttttga tgcttccctt tataagatgg aagtatccaa aatccttaac
     3241 aaagattgac tgaccaattg caacaacggc agaaattctt tttaagaata actcaaaaaa
     3301 tattttagcg tccgtatccg gcacaccaac attttttgca acttttctta ttaattccgc
     3361 tttagtcatt tttattatta gagattttac aaacgataat taatgccaat gataatattc
     3421 aatggaactt ccttatatcc attccagtaa taatttttct gattcagaac attatttagg
     3481 tctaaagtta aatcaagatt tggttgaaat gaatatataa atgagagacc taaatcaaaa
     3541 taattgccga gagagatatt gttttgaata tccgcatatc tatttgatag ataatcaagt
     3601 cttgctgtag tacttaatct attctgaaac ttataagtgt atgcagcatt aagtttaaat
     3661 gcaggcatat atggaatttg atttccatca ttatcatgaa tatcagaaag ttcaacagaa
     3721 ctgtataatt cacctgacgg accaagataa aataaaaagt tggcataagg acttatattt
     3781 ttcatatcag cataatgcag attaaactta cctgagtctg atgacggacc aaaataaggg
     3841 aatgcatcag aagtaaaata ttttaaaccg ccatcaatct ggaaatattt atcaaactca
     3901 tattttacgg acgcagtcaa agcgttactt ttctcccagt atatgcttcc gatactatca
     3961 actttgaaat aattattctt aacaacaaag cttccgggac tcataaattc agcagtaggc
     4021 gcatactcgc caaagagcgt aatgttttta tccagcttaa ttgcaacaga tgcgtaaagc
     4081 gcgttgtagg tttcgcctgc tccgtgtgaa aaagtccagc cgacagaacc tttaatcagt
     4141 tttgtaaaaa ataaaccagc agttggtctt aataagaaaa aatctttgcc agaattatcg
     4201 cccagcagat ttttaattga atgatttcta taatcaacag ccgcaccaat attaactgca
     4261 gaaagtttta acagtgcttc accttttatt ctcagattat tttcatcaaa ttcttcttcg
     4321 gaaatatttg ttacattatt tgtaaggttt aatccgaata aaaagttctt accaaaatca
     4381 ttctttacat caactacaaa tttcccataa tttaaagttc gtttcttggt aggattatca
     4441 gaagcaaaaa acttataagc agtagtgccg taatctccgt taacactaaa ttgcgttccc
     4501 ggtaatattt ctccatcaat atcagaccaa taagtaaaat taaatccaaa tcttgtgctg
     4561 aaacgatcgc tgttatcttc ataggctctt gtaaaatttc ctttagcaat tccctcgata
     4621 attccattcg taaaaggatg tgcataagta gcacttactg ttggaatagt gtacaatcca
     4681 ataccaacag caatattccc tttgtaaaaa ctaacatcgt ctaaaaaact catatcactt
     4741 tttaacggat tagaaaaatc tccgatctca agttcttcag gtgaataagt tggtttgaca
     4801 aaatcttcat ttacggatga tacaaaatca ggtttaattt tatcaaccct ctttatattt
     4861 aactggcttt tacctgtgat tacaaaatcg ggaagttcaa catttggatt tttagtttta
     4921 tcttcctgtg aataaacagc aaggatcgaa aatgataaaa tcaataatat ttttaacctc
     4981 attgcacttt cctcaatttt tgttgggctt cattaccaat cggcgttcca ctgtattttg
     5041 caattacagc ccgatacatt tctgcagcat ttcttttatc atttagttta acgtaacaat
     5101 cacctaacaa taaataggat ctcatcagcc attcttcata acgcgagtaa acagttctaa
     5161 ctctaaccag tgaagtgata gcttcattat attttccctg atcatacaga gactgaccaa
     5221 gataatattg cgattttgca cccagatcat ctgttctgtt ttcagaaagt gctgttaaat
     5281 actcatcggc actagaatat cttttagcag caatatcaat cagggctaat tcaaattttg
     5341 atttatcccc gaacactgta cctggataat acatgaccac ttcttcaaaa actgaatacg
     5401 cttcctgaaa attattcttt tcaagcaaag tcattccctt gttatactga aactcaggaa
     5461 ttttagccga tttttttaat tcattgatac cacgatcata aatagatatt gcagcatcat
     5521 aatttttcaa tgatcgataa ataagaccca tttcaagtac agatgccgaa gctaactctg
     5581 agtttttata tacatcgaaa actttttcaa aattaaatat tgcttcttca ttctggccca
     5641 agttctgagc acttttacct atccagtaat aaccatctgg aacaagtttg ctattaggat
     5701 aagcagatgt aaaactcatg tactcagttt tggctttatc atattgccgc aagctgtaat
     5761 aaatttctcc tttcttgaac atcagttgat cagcataact taaacttgga ttttttgata
     5821 caaaaccatc aagaaaagaa actgctttct ctggatttcc ttttgcaaca tagctgtatt
     5881 gtataccggt taccgcatca aaaacatatt gcgaagatgg aaattgtgat attacttttt
     5941 cataattttc aattgctaaa tcatattttt ccatattgaa ttgtgcatca ccaatagaat
     6001 aataaattat tggttctaat tttgtcttcg gatacttaga gataacatta ttgtactggt
     6061 caatagcttc agcatattct tttttctgaa aatgtatcca tcctattgtg taaagagaaa
     6121 cttcagcata ttcagaattt ggaaactttt cctgaatcaa tttgaattca tttatggctt
     6181 catcggtttt acctgattta taaagtgcct gagcgtattg atatcttgca tacggatctg
     6241 tacttgattt tgcatctgca ctgaataatt cctgataaac tttacttgcg gcagcataat
     6301 ttttgcttcc aaaataacta tccgccaatc ttaattttgc atcgcttaat cttttattct
     6361 taggatactt tttaatgtaa tccgcaaatt gataagctgc attttcataa tccccaagat
     6421 taaaataact gtaagctttc ccgtacaagg tttgagaatt aatttcctga tccgcaccgg
     6481 atgaagcatt gtaattattt agcgcatttt tataatcacc agtagcaaaa taattttcag
     6541 ctaagaaaaa attagttttt tgggtttcaa atcccggatc tgaattttct aattcattta
     6601 gataattaag agcagtttta ttatcgttaa ggtgatataa tgagattcca agtcctaata
     6661 atgctctgct gtgaactgat ttttcagcac cttgattgct gataacgata tcaaaataat
     6721 tcttggctgc tttaaagttt cctttattta actcgatctc accgagcatg gcaaaagatt
     6781 tagatttaat tatttggtca tcagaagaat ttgcagatat taaatacctt gatgcgagat
     6841 cagtcttatc attataaaag taaaccacac caagctgata ttgaacttct tgagcaagcg
     6901 gctcatctgg atatttctct aaaaattctt tatagatagc catagcttcg ttattttttc
     6961 ctaaataacg tttgcattca cctttccaga acaaagattt tattccaatg gtatctgatt
     7021 ctgttgatag attatcaaat tctgtgaaag cctgattata ttttttttgc tggaaatatg
     7081 tccaggctaa tccatattta gcattacgag catatttagc atcaggaaat ttttgtaaaa
     7141 cttcaaggta acttttttca gcatcgctgt attcaccaac ccgatagtat gatgtagcaa
     7201 gcagataaat actttcagaa tacatatcat cagaaagatt agtaagtctt ggacttttta
     7261 gttcaacaat agaagattga taatctttta acttgaaata acaaattccg atccttatct
     7321 gtgcagaagt agcaagtgtt gaattcttat gatatgtaag caactgatcg taatatttaa
     7381 cagcattggt ataatcacct gtttgttcgt aagcattcgc caaagcatag attgagtagt
     7441 cggcaaattt tttattacgt ttatcggcaa cagcttcttc caaaaattga attgcttcgt
     7501 caggtttatt ttctatatag aaagactctc ccacccagta aagactatta ccgtaataat
     7561 cactttcagg atactcattt aacaggagtt ttaatttgtt tcgtgataac tcatacttac
     7621 cggaactgta ataaatcaaa ccaagattgt ataatgcctt atcgcgataa ttagaccaat
     7681 tatacgtttt aactaaatat tcaaatccaa ttgcggcttc atcttttctt ccaagtttca
     7741 ataacgattc agcagaataa tattttgcag ttgcataaac ttcatcaaca attttatatt
     7801 cattaaaaaa atcttcgaac attctgtttg attgtgcgta ctgctggtta tgatatgcag
     7861 tcatcgcttc atcgtacttt tgagaagatg tttgagaata gacaggagaa ataattgtaa
     7921 taagaataat taacgaaaag aattttagca tactaaacct tcacaaagat ttgggtgaaa
     7981 acttaaaatc aacaatcaaa ttattaaaaa tgctcgtttt tattaaaaat catcgaatct
     8041 gaataatttt ctaaaatctg gattaaatat aaacttcaga attccataaa gtcaagctac
     8101 ttaataaaat atagagatct tctaaagaaa agtgttaaaa aaaacagctc aaattttaac
     8161 tttatttaag acgcagaagt gccaggattt gtttctctta tttgatgtct tcaagaaatt
     8221 tcagaatcag atttagatgt atttttagtt ttttttattt gatattcctc aaaaacacat
     8281 aatccaaatt ttacagaagg ttttgcagaa atcagttcaa ggtttttaat ggtttttaag
     8341 atctcattaa aatcagtgcc aacctgaaca gcgccattaa tatgagaata aagctggtct
     8401 ttaaatttta atgctgaaag aattgagacg atacaaactt ccctgtcttt taaagttagt
     8461 ccttcccttc ccaagacttt tccataacct tctactatta accattcagc catttccgga
     8521 gaaaatgatt ttacatttga tatcagctta tcgtacttat ttccataaat aatccggcaa
     8581 ttcatctcac ctctgcttaa atatttcttc aaatcatagc ctttatagac tttatccttt
     8641 cctgcaattt cgctcaatct ttttaaagat attaatgctg atggaaatcc tgcaaataaa
     8701 taagtctgta aaagtgcttc gtaaattttt ttcacagaat attttttgat taacagtact
     8761 tctagtatcg aatcgaagta tttttcttta cgcagcacag aagatgcact tagaagtgct
     8821 aaaagttcaa gctcagataa tttgtctaat ttcaattcaa atccagtatg ataaaaaaaa
     8881 accctcgcat caacgagggt ttataaaaat caaaataaat tattttatta aactaagttc
     8941 gtaatcggca gtctttttat atgttggatc agcagcagct aatttaaaat tatctttagc
     9001 ttttgctgta tcacctttgt atttgtatgc tattccaatg tagtaatatg gcccgccttt
     9061 agtaatattt gaacggtatt taagggcatt ttgtgcggct tcaatagctt cgtcatactt
     9121 gccggtttca gaatagactt ttgcaagcga taaataggct gcatcatagt tatcattttc
     9181 aacagatttt ttgagatatt caattccttt gttagcgtca ccaccagcta atgattcatt
     9241 accaagctta gcatatgcaa gtgaaatgtt tttcttaact tttgttttaa tagaattact
     9301 ggcattcgaa tttagaactt tttgaaaatt atcaatagcc gcctgataat ttcccattga
     9361 aaaataaact ccgcctaaag catttaaggc accttcatat ccgttattaa gtttaagaca
     9421 ttcctcgaag gctactttag catcttccag tttattagct tttttaagtg caacaccttt
     9481 ttgatagtag attctataat ccttttcaat tgctaaagct gcgttatatt tttcgatagc
     9541 atcattggca ttgccttcct ttaacagtgc atttccttca ttataaagtt tacctgcctc
     9601 tggtttcatt tcctgagcaa atcctaatat tgatatactc agtaaaagta aaacagataa
     9661 caataagccg tttttagtca tagtattttc ttcctattct atttagataa tggattattt
     9721 tgtgcgggag gcgggacttg aacccgcacg ccaattaagg cactaccccc tcaagatagc
     9781 gtgtctacca atttcaccac ttccgcaaat ttgagttgac aaattatggg tatgagttta
     9841 gaaaatcaac actttttaaa ggtcttataa taaaatttaa ttattacggg gctttctatc
     9901 aaaaagtaca tttctaatct catccctaaa ctttttctcg aagacaataa gtttagcgat
     9961 ttgttcttta gttaagatat catcaagtga ttttataaat tggttgcgct tggattcaat
    10021 agattcacga attttaagca tttctgatat taactttttg tacttctcat cttgattttt
    10081 atcggatgaa ttaaatgatt tttccagctc aaatattata tcatccgctt ttctttctaa
    10141 atcttgaatt tcccctcgcg aatcattacg ccttgcaaaa aaccttattg atgtttcttc
    10201 atccaaatcc aaagtttcaa taagcttaat tttttctaac tgttcgagtt tgtctctatt
    10261 cttcattcct ctttctctca tctcctgcga gaatgatatt gaatttaaaa gaactacagc
    10321 aacaaccatc atacataatt tgtacatatt tacctcttat aaaatctctt tattaattaa
    10381 ttgattataa atcatttcta catcattatc agaaagatat
//
LOCUS       DASWBY010000683        17049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_178875, whole genome shotgun sequence.
ACCESSION   DASWBY010000683 DASWBY010000000
VERSION     DASWBY010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 17049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17049
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178875"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /gene="lhgO"
                     /locus_tag="VF870_12440"
     CDS             complement(<1..846)
                     /gene="lhgO"
                     /locus_tag="VF870_12440"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX9253046.1"
                     /translation="MSNTYDITIIGGGIVGTATALSILENKKVNLLLLEAESSLAAHQ
                     TGNNSGVIHSGLYYKPGSLKAANCTLGREMMYDFCEQHSIPFNKCGKIVVATSKEEIP
                     ALNMLEERGKANGLSGIKRIPPEEIKQYEPHTYGIDGLFVPQTGIVDYIAVTNKYAEL
                     ICNKGGEIKTNSKLTSVKRDGKDLILFTEHDEYKTQFIVNCGGLQSDRIAKKFGIDPK
                     LQIIPFRGEYFKLKKEKEFLVNNLIYPVPDINFPFLGVHFTRMINGGVEAGPNAVLAF
                     KREGYK"
     gene            1036..1347
                     /locus_tag="VF870_12445"
     CDS             1036..1347
                     /locus_tag="VF870_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253047.1"
                     /translation="MNKNQKYVLAFGIILIAITLFFWYSQSAQVFTKSQVLVDKTTDL
                     DRMLGVENKQYVDQFVFGLFPSGMASIAEISSAASISGVIIVLTGFLIYLFRNKRKDK
                     K"
     gene            1344..1979
                     /locus_tag="VF870_12450"
     CDS             1344..1979
                     /locus_tag="VF870_12450"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9253048.1"
                     /translation="MKKTLSIFAVIMIFTASIFAQGFKVKATGEQTFNFEDKAGRNQT
                     TFFSTTPLEDITGLSGGVQGSVTFNVNDLKTLKGKLTVPVATIKTGIDTRDEHMKGAG
                     WLDATTYPEISFEIKKVSDVKVVANNKVTAKVTGNYTMKGVTKEVVADATLTYLDESE
                     QTKMRAPGDLLGVQAKFNVKLSDYGVNNKLVGQKVSENVEVSVNIVGSNAK"
     gene            2062..2586
                     /locus_tag="VF870_12455"
     CDS             2062..2586
                     /locus_tag="VF870_12455"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX9253049.1"
                     /translation="MNKTITIFGSAIPKKDDAQYKFAYQLGAALVQNGFNICTGGYGG
                     IMEAASKGAYDNAGFVYGVTINLWNKDPNPYITVEVREQKLFERITKLIELGDAYVIL
                     QGGTGTLLEFAAIWEYANKNLQQPKPIICHSNMWKEIVDVINKQMIIEERRTDLIKCC
                     DSVDEIVEYLKNNF"
     gene            complement(2661..3710)
                     /locus_tag="VF870_12460"
     CDS             complement(2661..3710)
                     /locus_tag="VF870_12460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9253050.1"
                     /translation="MKILILALSGIGDALMFTPALKLLRESLPSAQIYALVMYKGTKE
                     IYETNPNFNKVIYFDFLKEGALNSLKFVLSLRKKYDATINVYPSNRKEYNFINFLLGA
                     KKRVGVEYLRMNNSNFGWLNNVTVLENDKTHNVQSNIKLIEKLLNKKFDEEPAMEIHL
                     SEGHKKFADHYLETNSINQNDFVVGFHPGCATLKNHIKRRWEPEKFTELGKKLINEKN
                     AKVFLFGGLEEEELKSSINKMVDSENCFVIKTEKFLQSIAIMKRCNVFVTNDSALMHV
                     ASALGLKVIAIIGPTNQHYIYPWKTEHKIVSLNLECSPCFFYTPKPLTCSRTDVQFKC
                     IKELAVEMVYQNIYA"
     gene            complement(3818..6787)
                     /locus_tag="VF870_12465"
     CDS             complement(3818..6787)
                     /locus_tag="VF870_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX9253051.1"
                     /translation="MNFKLLNKVIAGIVFLISFIVLFMTVQPSVSFWDCGEFIAASVS
                     LQVPHPPGTPFFLLLGNIFSKLPIGENLGFRVNMISVISSALSILFLYLIAVKLIENY
                     KGKKADNMFDAISTYISAAIGALAFSFSDTFWFNGVEAEVYAFSTFLFAAVTYLIIRW
                     NERADNKDSEKYILMIAYLIGLATGVHLMSVLAAVPVVMIIMFKKYVNDEESLKKTGY
                     IFLAHSAIILLVAFFWWGSKKSQTPPTMEEYKDFDTKFKLIIAAISALIMGVYWKKIF
                     TRNSFYMPLIIGGIALFAIYPGVVKYLPELMTSIGGDNIVTEIIILILLFAGLGYGVH
                     YSRKESKPTLHLIFMSFIFVLVGFMTFAMVIIRSNQNPPMNENAPDTFTELVKYLNRE
                     QYGDFPTFKRRFATEPHQQIVYTGYSSDLDFFYTYQMNHMMTRYWLWNFAGREGWEQD
                     QGANIAPFNGIGNIFGKLIGVNFAGEVKDSLFGIPFLIGLLGIYFHFRKDWKMAAVFM
                     IMFIFMGHLTAFYQNQQQPQPRERDYFYVGAFFVYAIWIAIGLRGLIDLIQEKIKSVS
                     ARNAAAYAVLAVGIVLVPVKMLQANYFTHDRSNNWVPWDYSYNLLQSCAPNSVLFTNG
                     DNDTFPLWYLQDVEGVRRDVKIANLSLLNTDWYILQLKNNDPYKVGTVKMKLSDQQIK
                     DIRPIQWSARNITVPLPKPNNTVSFSDIMQQFALRDTSVLKQGEITWKMNPTLNYGNV
                     QAVRVQDLMVKEIVEANNWERPIYFAVTCSEDSKIGLSDYLRMEGMAFRLVPEKRRPN
                     DEFIEPNILQANLKEVVGYSKDYQPGFKFRGLNDPNIFFDDNHKRMVQNYRNAFLRLT
                     LYYIGQGKNELAVNTLDTMEEKLPNKLIPMDYGLLYEISNLYLRAGAKDKFNKFSAEV
                     EKLALAKLEKDPSDVQSYYNPYRILIDLYEAQGRNDKLLEIWQKLESIFPEDPNVKAN
                     IQKYRALVQDIDTSKVQ"
     gene            complement(6944..7177)
                     /locus_tag="VF870_12470"
     CDS             complement(6944..7177)
                     /locus_tag="VF870_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253052.1"
                     /translation="MAYRDQQKYLDFLRRFENKMDRNELEDYKKFVKRQKDDEDFDTV
                     SMTRLKDLYDKFYVPVDKSKYDHLFKKGNESEI"
     gene            complement(7501..8079)
                     /locus_tag="VF870_12475"
     CDS             complement(7501..8079)
                     /locus_tag="VF870_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9253053.1"
                     /translation="MKKIVVLIFIIVITSLSYSQSKISVGLGYDVALPAGDFADIAKT
                     GYSWTLFGEYRISPQYSVQLLSSYMIFPVDIEPVAAQGKVITFDLKSIPIKGAVKYFL
                     YDEFFLVGEIGANFIKVSADFQDSYGGTTNESTDYTAKFTLGAGLGTVFRLSDQAQIN
                     ITAKYDFVDGGDSSIDFSHFLIGAGLVFHFNI"
     gene            complement(8076..8429)
                     /locus_tag="VF870_12480"
     CDS             complement(8076..8429)
                     /locus_tag="VF870_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253054.1"
                     /translation="MRKIFPIIVGLLIIFGFYYLASTSGSGDKIYRELDQSIKSDYTQ
                     LKISRAQLKITQQDYITGLKQLSIKEDDLFNEVRLHKFEDIKEHNYWHRSRLKFPSSI
                     KMELDLITKAEKDST"
     gene            8572..8874
                     /locus_tag="VF870_12485"
     CDS             8572..8874
                     /locus_tag="VF870_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX9253055.1"
                     /translation="MIKHIVFWRLNESAYGNDKKTNAQILKDKLFAMQGKVDGLLKLE
                     VGFDFSNEKDSCNVVLYSEFENKEALHQYQIHSDHQEIKKWLSEVRYERRVVDYEI"
     gene            8864..9601
                     /locus_tag="VF870_12490"
     CDS             8864..9601
                     /locus_tag="VF870_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9253056.1"
                     /translation="MKFNLKKINLNSLLLVFLLTISLVENIYSQINDFEEKGTRTIYL
                     IRHGHYDEADERDEFTGKELTPLGITQARLVAARLEAMPVDFTSLISSTMTRARQTAM
                     IINQEFPELELKQNALLSECTPPTWRKDIMAKTDSVEAIDCVENIEKAFQEFFVPSPD
                     ENDRNDILVCHGNVIRYFVTKVLKVDSLSWLQMSITNCSLTIVRITPDGSMKLDAFSD
                     YGHIPENLRTFTGGKNEEKELVIPVKH"
     gene            complement(9642..13061)
                     /gene="mfd"
                     /locus_tag="VF870_12495"
     CDS             complement(9642..13061)
                     /gene="mfd"
                     /locus_tag="VF870_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX9253057.1"
                     /translation="MNKSNTSNINFISFDAFQKLSEATNAKIIFISPLYGSSKSFAVK
                     DLFEKNEQLVLLFVDGKTVHETKVELSVLGLSDDLIVIDDFNLESIQEKITEISKRKK
                     FILLTTYEILNLKLPDKEKINHLTTKVSVGGDLKYDDLIEYLNLLVYTKDKFVEAPGY
                     YSQRGAIVDFWSYSEKSPVRLEFDGDFIESIRYFDSESQRSFEKLEEVTLAGAFENQA
                     AEFSSDIFGYLNNPIFIASSYELDNIKSKKEELTPVVEEEIRSKKKGKIPDEDEFPEP
                     SHSELISETENLKKDLPDESQAQEQKTSFDYKSTNARWIIEEELTSKKRIELGISPAP
                     AINSNYQILFSILKEYADKSFNIIITSENELQTKRLSELLTDINSELEEFIESDKIKI
                     ETLAIKDGFVNRAEKILLLTDYHIFNKPYRTKISSAKKYKKSKAKSFASIKRDDYVVH
                     EDYGIGQYSGLQTIKIGDVDQESMKILYAEGGVVYVNLNYLALVKRYSSNENLKPTLA
                     TLGSGEWQNTKAKAKKKIKEAARELIELYAKRKSTEGYKYSPDTVWQKELEASFFYED
                     TPDQAKASEDVKLDMEAKNPMDRLVCGDVGFGKTEVAVRAAFKAVQDGKQVAVLVPTT
                     ILAEQHYNTFKDRLTQFPVRVAALSRFQTKKEQKEIVKLLEDGQLDVLIGTHRLISKD
                     VKFKDLGLLIIDEEHRFGVAAKEKLRQVKVNVDTLTLTATPIPRTLNLSLLGARDLSI
                     IATPPPNRQPIFTNVSTFDALKIREWIMNELKRNGQVYLVHDRVQSIDKLADYIKRYV
                     PEAKIAVAHGQMKPSQLEDVIHGFLNRKFDVLLSTKIIESGIDIPNVNTIIINRADRF
                     GLAELHQLRGRVGRSDRQAYAHFIVPSLSGITKKALRRLQAIEEYTEIGSGFNVAMRD
                     LEIRGAGNLLGTEQTGFINDVGFDLYVKLINEAVEELKYQDFKEVFKSLPKIEERTDP
                     TIDTFFEIGIPVTYMPEQMDRLNFYTALYSVKSLSEIEELKEEMLDRFGVLPEIVKRL
                     VLTATLKFYSSFALFERVAIQRKNIFIILPRGEKEDYYKVRFVELMRFIMDEYKDKIK
                     FNQQKEVMKLVIENRFEKAEDILSYLITFCGRVSKLFGNEVKLNSDTEEAA"
     gene            complement(13122..13898)
                     /locus_tag="VF870_12500"
     CDS             complement(13122..13898)
                     /locus_tag="VF870_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9253058.1"
                     /translation="MKYFLLFFIVPLHFTFAQADSLIIHDFNNFLKVGADFYTSPLRF
                     DSKDWVKLSSVVGISALASLADKEIKNFSQRNQSESADALFNIDKYYYAEFVGASIIA
                     FYGYGLVAENNKIRKLAVKLTEATFLATSLTIITKTIVGRGRPYMQESQYSFNPFTMD
                     NDYNSFPSGHTTLVFAYSTVMANEVDNVFWKIGWYSAAGLVGYARIYHNQHWFSDVLM
                     GAAIGYFSGEFVNNHNSNIGNDVSINLYPLPMGFAMQIVF"
     gene            complement(13924..14610)
                     /gene="rsmG"
                     /locus_tag="VF870_12505"
     CDS             complement(13924..14610)
                     /gene="rsmG"
                     /locus_tag="VF870_12505"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX9253059.1"
                     /translation="MPPTKQDNYLKELQSFCWDNGFNPEPMRNERLAFFAQLVAEKNK
                     TVNLISRKDIDSIIENHIFISAYVTKYLPEKVTRFLDIGTGGGFPGIPLAIMRPDLRG
                     VLVDSTGKKIDAVKEFLDKLKLSNAVAENARVESPEFIEKYKGSFDLIVSRAVVPIII
                     LFRYSLPLVKDKAFIVTIKGGDLDDEFKKAELKYKSFIKKSTIYELAYKPTNLRNKKG
                     KKLVVIELQK"
     gene            complement(14627..16504)
                     /gene="mnmG"
                     /locus_tag="VF870_12510"
     CDS             complement(14627..16504)
                     /gene="mnmG"
                     /locus_tag="VF870_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX9253060.1"
                     /translation="MKKFDVIVVGGGHAGIEAASAGARMGCSVGLVTMDRYALGRMSC
                     NPAIGGTAKGHLVREIDALGGVMGQIADQTGIQFRMLNKSKGPAVWSPRCQSDRKLYS
                     EEAYRKISQTQNLEIVEESAVDVIAEDGKVVGIKTQSGEEILCKALILSSGTFLNGLM
                     HTGLNKTSGGRFGEQPALGITESLAKLGFDYGRLKTGTPPRLKKDSINWEILEEQPGD
                     ENPQPFSHQTDMSNFPFLPQVSCHITYTDPLVHKILEKGFAASPLFTGLIKGVGPRYC
                     PSIEDKIVRFSDKERHQLFLEPEGLDSDLIYLNGFSSSLDADIQLEALHKIVGLENVE
                     MVRPGYAVEYDFFPPHQVDLTLETKLIKGLYFAGQINGTSGYEEAAAQGLVAGINAAL
                     KIQGKPEFVLKRSEAYIGVLIDDLVTKSTDEPYRMFTSRAEHRLLLRQDNADRRLMKY
                     GYEFGLIPKNLYDNVDNREKTIIDGINYCHTKKYHARDINQFLINSGSNEIDSTETIS
                     KLCKRPEFNLKQLLEINGTANDLEAVALLQDEKALEQAEIEIKYEGYILRQHEAAGKL
                     EKYESTKIPLDYDFSKIKQLSTEGKEKLNKVKPRSIAQASRISGVTPSDISILLVYLK
                     N"
     gene            complement(16586..>17049)
                     /locus_tag="VF870_12515"
     CDS             complement(16586..>17049)
                     /locus_tag="VF870_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX9253061.1"
                     /translation="RSADIVIYLEDVQQEESKDLFVDLLNFTSHDKIIKAINKIDLDK
                     NISKEADIKISAKTGEGIEALFGKLKEKTVGNESYTEKTAIVTNLRHHNCLKKSRENL
                     INARESILKKMSGEFISVDLRNAEMNLAEIIGEVTSDDILNNIFMKFCIGK"
BASE COUNT         5525 a         3118 c         2717 g         5689 t
ORIGIN      
        1 tttatagcct tcacgtttaa aagcaagcac ggcatttggt ccggcttcaa ctccgccatt
       61 tatcatcctg gtaaaatgga ctccaagaaa aggaaaatta atatccggaa cgggatagat
      121 aagattattt acaagaaatt ctttttcttt tttcagttta aaatattcac cccgaaaggg
      181 aattatttga agcttgggat caataccaaa cttttttgca attctatctg attgcaagcc
      241 accacagttt acaataaact gagttttgta ttcatcgtgc tcagtaaata gaattaaatc
      301 tttaccatct ctttttactg aagttaattt agagtttgtt tttatttctc cacctttatt
      361 acaaatcaat tcggcatatt tgtttgtaac agcgatgtaa tctacaattc ctgtttgagg
      421 aacaaatagt ccatctattc cataagtatg cggttcgtat tgttttattt cctcaggagg
      481 aatacgtttg attcctgata gaccgttggc tttgcctcgc tcttcaagca tattaagggc
      541 tggaatttct tctttgctgg tggcaacaac aattttccca catttattaa acggaatact
      601 atgctgctca cagaaatcat acatcatttc tcgtcccaaa gtgcagtttg ctgctttaag
      661 tgaacccggt ttgtagtaaa gtcctgagtg aatcacaccg ctgttatttc cggtctgatg
      721 tgcggcaagg gaagattcgg cttccaataa aagcagattt acttttttat tctccaagat
      781 ggatagagca gttgctgttc caacaattcc accgccaata attgtaatat cgtaagtatt
      841 gctcatatgt gatctataca acagatttta agttaataat aaaatggctt gattatttga
      901 tttgtgtgtt ataacataca atagtgcttt aagaaaatta ttttattaat taaagtccct
      961 ttactaaacc aaatttaact aaatcatatg agcaattaaa taattttctg gattttaaac
     1021 agttggaatt ataaaatgaa taaaaatcaa aaatatgttc tagcatttgg aattattctt
     1081 attgcgatta cgttattctt ttggtactcg caaagtgccc aggtttttac taaatcccag
     1141 gtacttgttg ataaaaccac cgatcttgac agaatgcttg gtgttgaaaa caaacaatac
     1201 gttgatcaat ttgtttttgg tctatttcct tcggggatgg catcaattgc ggaaataagc
     1261 tccgcagctt caatatcagg agtaattata gtattaacag gatttttaat ttatttattt
     1321 agaaacaaac gaaaggataa aaaatgaaaa aaactctctc aatctttgca gtgataatga
     1381 tttttactgc atcaatattt gcacaaggct ttaaagttaa agcaactggc gagcagactt
     1441 ttaattttga agacaaagcc ggaagaaatc aaacaacatt ctttagcaca actccacttg
     1501 aggatattac aggtttaagc ggtggagtgc agggaagtgt tacttttaat gttaacgatt
     1561 tgaagactct taaaggaaaa ttaactgttc ccgttgcaac aattaaaaca ggaattgata
     1621 cacgcgatga gcatatgaaa ggcgctggat ggttagatgc aacaacttat ccggaaattt
     1681 cctttgaaat caaaaaagtg agcgatgtaa aagttgttgc aaataataaa gttactgcaa
     1741 aagtaaccgg caattacaca atgaagggcg taaccaaaga agtagttgct gatgcaaccc
     1801 taacttatct ggatgaaagc gaacaaacaa aaatgcgtgc acctggagat ttacttggtg
     1861 tgcaggcaaa gtttaatgtt aagctttcag attatggtgt taataataaa ttagttggac
     1921 aaaaagtttc agaaaatgta gaagtaagtg tgaatatagt tggctcaaat gctaaataag
     1981 tgaaaatgat gatatggtta aaaacccgat tcttaaatga gtcgggtttt tttattttag
     2041 ggcatcacaa ttttactact tatgaacaaa acaattacaa tcttcggaag tgcgattcca
     2101 aagaaggatg atgcacagta taaatttgct tatcaacttg gcgcagcttt agtacaaaat
     2161 ggatttaata tttgtacagg cggttacggc ggaataatgg aagcggcatc aaaaggtgct
     2221 tatgataatg ctggttttgt ttacggcgtt actatcaatc tttggaacaa agatcccaat
     2281 ccatacatta ctgttgaggt tagagaacaa aaactttttg aacgcattac aaaacttatc
     2341 gaactcggtg atgcttatgt tattctgcaa ggtggaacag gaactttgct agagtttgcg
     2401 gccatctggg aatatgcaaa caaaaatctt cagcaaccta agcccataat ttgccattcg
     2461 aatatgtgga aagaaattgt tgatgtaatt aacaagcaaa tgattattga agagagaaga
     2521 accgatttga ttaagtgctg cgatagcgtt gatgaaattg ttgagtattt gaaaaataat
     2581 ttttaatact agttcttcaa cctgaatttg tttcaggctc tcttaattat taatcagatt
     2641 ccgaaataag ttcggaatga ctatgcataa atattttgat aaaccatttc aacagccaat
     2701 tccttaatac atttgaactg aacatcggtg cgggaacagg tgagtggttt aggtgtatag
     2761 aagaaacagg gcgagcattc taaatttaac gaaacaattt tatgctctgt tttccatgga
     2821 taaatgtagt gttggtttgt tggaccaatt atcgcaataa cttttaatcc caatgcggaa
     2881 gcaacgtgca taagtgctga atcattagta acaaaaacat tacatcgttt cattattgca
     2941 atgctttgaa gaaacttctc tgttttaata acaaagcaat tttcagaatc aaccatttta
     3001 ttgattgatg attttaactc ttcttcctcc agtccgccga ataaaaacac ttttgcattt
     3061 ttttcattga taagtttttt accaagttca gtaaactttt ccggctccca tcttcgctta
     3121 atatgatttt ttaatgttgc acatcccgga tgaaacccta caacaaaatc attttggtta
     3181 atgctgttgg tttctaaata gtggtctgca aactttttat gtccctcaga aagatgaatt
     3241 tccattgctg gttcttcatc aaacttttta ttcaataact tttcaatcaa cttaatattt
     3301 gattgaacat tgtgtgtttt gtcattttcc aaaaccgtaa cattattaag ccagccaaaa
     3361 tttgaattat tcattctaag atattcaacg ccgactcttt tctttgcacc aagtaagaaa
     3421 tttataaagt tatattcttt tctgttggat gggtaaacat taatcgttgc atcatatttt
     3481 tttcttaatg ataaaacaaa ttttaatgag tttagcgcac cttcttttag aaaatcaaaa
     3541 taaattactt tgttaaagtt tggattggtt tcataaattt cttttgtccc cttatacata
     3601 acaagtgcat aaatctgagc agagggaagt gattcgcgta gtaattttaa tgcgggagtg
     3661 aacatcaacg catcaccaat tccagaaagt gcaagaatta agattttcat gcgaaaatta
     3721 aattaaaaag atgtttaaaa aagtcaatcc cgaaaaaggg gaatctatag tttgcataaa
     3781 atagattccc gccttcgcgg gaatgactac gaaaaaatta ttgtactttg ctagtgtcta
     3841 tgtcttgaac aagggctcgg tacttttgaa tatttgcttt tacattcgga tcttctggaa
     3901 aaattgattc cagtttttgc caaatttcta aaagcttatc atttcttccc tgcgcttcat
     3961 aaagatcaat caatatccta tatggattat aatatgattg aacatccgat ggatctttct
     4021 ctaattttgc aagcgcaagt ttttcaactt cagcagaaaa tttattgaac ttgtcttttg
     4081 caccagcgcg caaataaaga ttgcttatct cataaagtaa tccataatcc attgggatta
     4141 atttattagg aagtttttct tccattgtat ctaatgtgtt tacagctaat tcatttttac
     4201 cttgtccgat gtaataaagt gtcaatctta aaaaggcatt tctataattc tgtaccatgc
     4261 gtttgtggtt atcatcaaaa aatatatttg gatcatttaa tccacggaac ttaaatccag
     4321 gttgataatc cttgctgtaa ccaacgactt ctttaagatt tgcctgcagt atattgggtt
     4381 ctataaattc atcatttggt ctgcgttttt caggaaccaa acggaaagcc attccttcca
     4441 ttcttaaata atcacttaaa ccaattttgc tgtcttcaga acaggtaact gcaaagtaaa
     4501 ttggcctctc ccagttattt gcttcaacta tttctttaac cattaaatcc tgcactcgga
     4561 cagcctgtac attaccataa ttaagtgttg gattcatctt ccaggtaatc tcgccttgct
     4621 ttaataccga agtatcacgc aaagcaaatt gctgcataat atcagagaaa gaaactgtat
     4681 tgtttggctt tggaagtggt acggtaatat tacgtgcgga ccattgtatt ggccttatat
     4741 cctttatttg ctgatcactt aacttcattt taactgtgcc aactttataa ggatcattgt
     4801 tctttaattg taatatatac caatcagtat ttaacaaact aagattcgca attttaacat
     4861 cacgccttac accttcaaca tcctgcaaat accagagagg aaaagtatcg ttatcaccat
     4921 ttgtaaaaag cacagaattt ggagcacagc tttgcaaaag attatatgaa taatcccaag
     4981 gaacccaatt gttcgatcta tcatgagtaa aataatttgc ttgtaacatt ttaaccggca
     5041 ctaaaactat tccaacagca agcactgcat aggcagccgc attgcgggca gaaacacttt
     5101 taattttttc ctggatcaaa tcaatcaatc ctctaagccc gattgcaatc caaattgcat
     5161 aaacaaagaa agcacccaca taaaaataat ctctttccct tggctgaggc tgctgctgat
     5221 tttgatagaa cgcagttaag tgtcccataa aaataaacat tatcataaat acagccgcca
     5281 ttttccaatc ttttcggaag tggaaataaa ttccaagtaa cccgatcaag aatggaatac
     5341 cgaataatga gtcttttact tctccagcaa aattaactcc gataagtttt ccaaaaatat
     5401 ttccgatacc attaaacgga gcaatatttg cgccctgatc ctgttcccat ccttcacgac
     5461 cagcaaaatt ccacagccaa tatcttgtca tcatatgatt catctgatat gtgtaaaaga
     5521 aatcaaggtc tgatgaatag cccgtgtaaa ctatttgctg atgaggttcg gttgcaaacc
     5581 ttcttttgaa agtagggaag tcaccgtact gttcacgatt aagatatttt acaagttcag
     5641 taaatgtatc cggtgcgttt tcgttcattg gcggattttg atttgagcga atgataacca
     5701 ttgcaaaggt cataaatcca acaagaacaa aaatgaatga cataaaaatg agatgtaatg
     5761 tcggcttgga ttcttttcga gaatagtgaa caccataacc tagtccggca aatagcagta
     5821 taagaataat tatttctgta acaatattat ctccgccaat actggtcata agttcaggta
     5881 gatattttac aacaccggga tatattgcaa ataaagcaat cccccctata atcaaaggca
     5941 tataaaaaga atttcttgta aagattttct tccaataaac acccataatt aaagcactaa
     6001 ttgccgctat aataagctta aactttgtgt caaagtcttt gtattcttcc attgttggcg
     6061 gagtttgaga ttttttagaa ccccaccaga aaaatgccac caatagaata atagcagaat
     6121 gagccaggaa tatataacct gtctttttta aagactcttc atcattaaca tattttttaa
     6181 acataattat cattacaaca ggcacggcgg caagcacact cattaaatgc acacccgtag
     6241 ccaggccaat aagatatgca atcatcaaaa tatatttttc gctgtcttta ttatcagcac
     6301 gctcattcca tcttataatt aaatatgtaa cggcagcaaa gagaaaagta ctaaaagcat
     6361 aaacttcggc ttcaacacca ttaaaccaaa aggtatcact aaaagaaaag gcaagagcac
     6421 cgattgcagc agaaatataa gttgatattg catcaaacat attatcagct tttttgccct
     6481 tatagttctc aatcagtttt actgcgataa ggtaaaggaa gagtatggac aacgctgaag
     6541 agataacaga aatcatatta actctaaagc caagattttc accaatcgga agttttgaaa
     6601 aaatattgcc aagcagtaaa aagaatggag ttcctggagg gtgaggaacc tgcagcgaaa
     6661 cagaggctgc tataaactca ccgcaatccc aaaaagaaac cgacggctga acagtcatga
     6721 aaagaacgat gaaagaaatt aaaaaaacaa ttcctgcaat aactttatta agcagcttga
     6781 aattcattgt tacctctaaa tagaatagac taaaaatata ttccactctt cgactacact
     6841 cagagtgaca aaataatact ctcttgtcat actgagcgaa gtcgaagtat gaccgttttt
     6901 taactacaca atataaatta taataaaatt tgtttttaga cgattaaatc tctgattcgt
     6961 tgcctttttt gaataaatga tcgtactttg atttatcaac cggaacataa aatttatcat
     7021 aaaggtcttt aagtcttgtc atcgaaacag tatcaaaatc ttcatcatct ttttgtcgtt
     7081 ttacaaattt tttgtaatcc tccagttcat tacgatccat tttattctca aatcttctga
     7141 gaaaatccaa atatttttgc tgatctctgt atgccattgg gaataccttt cttgttggat
     7201 gagcaaaatt aacagtgcaa aaaataaaca aactttaagt ggcaaaaaaa tgaatttata
     7261 tgcctattta attatgtttg gtaaaaattt tcaatcgttt taatatcaaa gaaaatgatt
     7321 atcagggcag aaaaattggg cgatgaatta tcaatttgga aaattaatat aagggtattt
     7381 ggtcagacta cagaagcaaa tattgttgat cagctgcgaa aaagatgtaa aaggtaaaat
     7441 gagttgctaa atatcgtcct gagtttgctg aatcagttta atatttagca acttaaatca
     7501 ttaaatattg aagtgaaaaa ctaatcccgc tccaattaaa aaatggctga aatcaatact
     7561 tgaatcaccg ccatcaacaa aatcatattt tgcggtgata ttaatttgcg cctgatcaga
     7621 caaacggaac acagtaccca aacctgcacc cagagtaaac ttagccgtat agtctgttga
     7681 ttcgtttgtt gttccgccat aggaatcctg aaaatcagca gatactttta taaagttggc
     7741 tccgatttct ccaactaaga aaaattcatc ataaagaaaa tatttaactg cgccttttat
     7801 tggaattgat ttcaaatcaa atgttataac cttcccctgg gctgcaaccg gttcaatatc
     7861 aactggaaat atcatatagc tggataataa ctgtacactg tattggggac ttattcgata
     7921 ctcaccgaat aaagtccagc tgtatcctgt ttttgcaata tcagcaaaat cccctgcagg
     7981 taaagcaaca tcgtatccca gaccaacact tattttgctt tgtgaataag aaagtgaagt
     8041 tataactatt ataaaaatta gtacgacgat ctttttcatg tagaatcctt ttctgctttt
     8101 gtgattaagt ctaattccat ttttatgctg ctgggaaatt tcaatctgct gcgatgccaa
     8161 taattgtgct ctttaatatc ttcaaacttg tgaagtctaa cttcgttaaa caaatcatct
     8221 tcttttatgc ttaactgctt taaccccgta atataatctt gttgcgtaat cttcaattgt
     8281 gcacgactaa tctttaattg agtgtaatca gatttgatcg actgatcaag ctctctgtaa
     8341 atcttatcac ctgatccaga tgtagatgct aaataataaa atccaaaaat tataagtaac
     8401 ccaactatta tgggaaatat ttttctcatt aaagattaat ttcttgtgca taataatata
     8461 tgaaataatg aattcgttaa aaatattgct caaataagat tgtttactaa cttgcagtta
     8521 tataattaga tagttttatt tttataaaag attattaata acaggagttt tatgataaaa
     8581 cacattgtat tctggcgctt gaatgaaagc gcttatggaa acgacaagaa aaccaatgca
     8641 cagatattaa aagataaact ttttgcaatg caaggaaaag ttgatggttt actaaaattg
     8701 gaagtcggat ttgatttcag taatgaaaaa gattcctgca atgttgtatt gtactctgag
     8761 tttgaaaaca aagaggcttt gcatcaatat caaattcatt ctgatcacca agaaattaaa
     8821 aaatggctaa gcgaagtgcg ttatgagcgc agagttgtag attatgaaat ttaatttaaa
     8881 aaaaataaat cttaactcgc ttctgttagt ttttctttta acaatttctc ttgtcgaaaa
     8941 tatttacagc caaattaatg attttgaaga aaaaggaaca agaacaattt atcttatccg
     9001 ccacgggcat tatgatgaag cagatgagag agatgaattt acagggaaag aactaactcc
     9061 tcttggaatt acacaagcaa gattagttgc ggcaagattg gaagcaatgc cagttgattt
     9121 tacttctctt atcagcagca caatgacccg tgcaaggcaa actgctatga ttatcaatca
     9181 ggaatttcct gaattggaat taaaacagaa tgcattatta agcgaatgca ctccgccaac
     9241 ctggcgaaaa gatataatgg caaaaacaga ttccgtagaa gctattgact gtgttgaaaa
     9301 tattgaaaag gcatttcagg aattttttgt tccatctccg gatgaaaatg acagaaatga
     9361 tattctcgtt tgtcatggaa atgttatccg ttattttgtt acaaaggttc ttaaggtcga
     9421 ttcattatcc tggttacaaa tgagtataac gaattgcagt ttaactattg ttcgtataac
     9481 accagatgga tcaatgaaac tagacgcttt cagtgattat gggcatattc cggaaaatct
     9541 gagaacattt actggtggga aaaatgaaga aaaagaattg gtaattcctg taaaacatta
     9601 gtgaacaaag aggttggcaa aggcactact gtttaaaaac actacgctgc ctcttcagta
     9661 tcagagttta actttacttc gtttccaaac aacttagaaa ctctgccgca gaaagtaatt
     9721 aaataagaaa gaatatcttc agctttttca aaacgattct caataacaag tttcattacc
     9781 tctttctgct gattaaattt aattttgtct ttgtattcat ccattataaa tctcatcagc
     9841 tcaacaaatc gcactttgta gtaatcttct ttttctccac gcggaagaat tataaaaata
     9901 ttttttctct ggattgcaac cctttcaaac aatgcaaatg aagaatagaa ttttaatgtg
     9961 gcggtaagaa ccaatcgttt tactatttct ggaagcactc caaatctatc aagcatttct
    10021 tctttaagtt cttcaatctc ggataaactt tttacggaat acaatgcagt gtaaaaattt
    10081 aagcggtcca tttgttcggg catataggtt acaggaatac caatttcaaa aaatgtatcg
    10141 attgttggat ctgttctttc ttcaatcttc ggtaaggatt taaagacttc tttaaagtcc
    10201 tgatacttca gttcctccac agcttcgttg atgagcttaa cataaagatc aaaaccaaca
    10261 tcgtttataa atcccgtctg ctctgttccg agtaaatttc cggcaccacg aatttcaaga
    10321 tcgcgcatag caacattaaa tcccgaacca atctcagtgt attcttcaat tgcctgcaag
    10381 cgtcgcaacg ctttttttgt aatcccgctt aatgatggaa caataaaatg cgcataagcc
    10441 tgcctgtcgc ttcttccaac ccgtccgcgc aattggtgca gttctgcaag tccaaatcta
    10501 tcagcacggt ttataattat tgtgttaaca ttcggaatat caatacccga ctcaataatt
    10561 tttgttgata acagaacatc aaacttacga ttaagaaaac catgaattac atcttcaagt
    10621 tgtgaaggtt tcatctgccc gtgtgcgact gcaattttag cctcaggcac atatcgcttg
    10681 atgtaatctg caagtttatc aatagattga acacgatcgt gaaccaggta aacctgtccg
    10741 tttcgtttca attcgttcat tatccattcc cggattttaa gcgcatcaaa agttgaaaca
    10801 ttagtgaata tcggctgcct gtttgggggc ggtgttgcaa tgattgaaag atcacgagca
    10861 ccaagtaaag ataaatttaa tgttctcgga attggagttg ctgttaatgt aagcgtatca
    10921 acattaactt ttacctggcg taatttttct tttgcagcta caccaaaacg atgctcttca
    10981 tcgataatca gtaaaccaag atctttaaac ttgacatctt tagaaattaa tctgtgcgtc
    11041 ccgatcaaaa catcaagctg accatcttct aaaagtttaa caatttcctt ttgctctttc
    11101 tttgtttgaa atcttgaaag cgcagcaaca cgaacaggaa attgtgttaa ccgatcttta
    11161 aaggtattat aatgctgctc tgctaaaatt gtagtcggaa caagtacggc aacctgtttt
    11221 ccatcctgaa cagccttaaa agcagcacga acagccacct ctgttttacc aaatccaaca
    11281 tcaccacaaa ccaaacgatc cattggattc tttgcttcca tatcaagctt aacatcttcg
    11341 cttgcttttg cctgatcggg agtatcttca taaaagaaag atgcttcaag ctctttttgc
    11401 caaactgtgt ccggggaata tttatagcct tcagttgatt ttctttttgc atacagctca
    11461 ataagctcgc gagctgcttc tttaattttt ttctttgcct tagcttttgt gttttgccat
    11521 tcaccgctgc caagtgttgc aagagttggt tttagattct catttgatga atatctttta
    11581 accaaagcaa gataatttag atttacataa acaactccgc cttcggcata tagaatcttc
    11641 attgactcct gatcaacatc gccaatcttt attgtttgca atcccgagta ttgcccgatt
    11701 ccataatctt catgaacaac ataatcgtcg cgtttaattg aagcgaaact ttttgcttta
    11761 gatttcttat actttttcgc agacgaaatt tttgtacgat aaggtttatt aaatatgtga
    11821 tagtcggtaa gcagcaaaat cttttcggcg cgatttacaa atccatcttt aattgcaagc
    11881 gtttcaattt taattttgtc tgattcaata aactcttcaa gttcagaatt tatatcagtt
    11941 aataactcac ttaatctttt ggtttgaagt tcattttcag aagtgatgat aatattaaaa
    12001 cttttatcag catactcctt taaaatagaa aacagtattt gataattaga attgatcgca
    12061 ggggctggtg aaataccaag ttcaattctt tttttagatg ttaattcttc ttctattatc
    12121 cagcgagcgt ttgtgctttt gtaatcaaag gatgtttttt gttcctgcgc ttggctttcg
    12181 tcaggcaagt cttttttcaa attctcagtc tctgatataa gttctgaatg acttggctcc
    12241 ggaaattcat cttcatcggg aattttccct tttttctttg atctaatttc ctcttcaaca
    12301 acaggagtaa gctcttcttt tttggatttg atattatcca actcataaga agaagcgata
    12361 aaaattggat tattcagata cccaaaaata tctgagctga attctgctgc ctgattttca
    12421 aatgcaccag caagcgttac ttcttcaagc ttctcaaatg atcgctggct ttcagaatcg
    12481 aagtaacgaa ttgattcaat aaaatcgcca tcaaactcca atcgcaccgg acttttttct
    12541 gagtatgacc aaaaatcaac aatggctcca cgctgcgaat aataaccagg tgcttcaaca
    12601 aatttatctt tcgtgtaaac tagtaagttt aaatattcaa tcaagtcatc atatttcaaa
    12661 tcaccgccaa ctgaaacttt agttgtaaga tgatttattt tttctttgtc gggaagtttt
    12721 agattcagaa tttcgtacgt tgtaagtaaa ataaattttt tacgtttaga tatttctgtt
    12781 attttttctt gaattgattc gagattgaaa tcatctataa ctatcaagtc atcagacaga
    12841 ccaagaacgc ttaactccac tttagtttcg tgaactgttt tgccatctac aaaaagtaaa
    12901 actaactgct catttttttc aaataaatct tttacagcaa aacttttaga ggaaccataa
    12961 agcggactta taaaaataat ttttgcatta gtggcttcag ataatttttg aaatgcatca
    13021 aacgaaatga aatttatatt tgaggtgtta gatttattca tattaaacga ggtataatct
    13081 taaaggcaat cagttgtcgc aattacatca gaattaagcg attaaaaaac aatctgcata
    13141 gcaaaaccca ttggcaaagg atatagattt attgatacgt cattaccaat attgctgtta
    13201 tgattattta caaactcgcc agaaaaataa ccaattgccg cgcccattaa aacatcggaa
    13261 aaccaatgct gattgtgata aattcgtgca tatcctacca agcccgctgc tgagtaccaa
    13321 ccaattttcc aaaacacatt gtcaacctca ttcgccatta ctgtagaata agcaaatacc
    13381 aatgtagtgt gtcccgaggg aaatgaatta tagtcgttat ccattgtgaa aggattaaat
    13441 gaatactggc tttcctgcat ataaggtctt cccctcccga ctatcgtttt tgtgataatt
    13501 gtaagtgaag ttgctaaaaa tgttgcttcg gtaagtttaa cagcgagttt acggatctta
    13561 ttgttttctg caaccaaccc gtaaccataa aaagcgataa tactggcgcc gacaaattca
    13621 gcataatagt atttatcaat attaaaaaga gcatctgcag attcactttg gtttctttgt
    13681 gaaaaatttt ttatttcctt atcagccaaa gatgccaggg cacttattcc aacaacagat
    13741 gaaagcttta cccaatcctt agaatcaaat cttagcggtg aagtataaaa atccgctcca
    13801 acttttaaaa aattattaaa atcatgaatt attaaactat ctgcttgggc aaatgtaaaa
    13861 tgcaaaggaa ctataaaaaa caaaaggaaa tatttcatta tttattaatc gaattactaa
    13921 aaattatttt tgtaattcta ttacgactaa cttcttccct ttcttgtttc ttaagtttgt
    13981 gggtttatat gcaagttcgt agatggtaga tttttttatg aaagatttgt atttcaattc
    14041 tgctttttta aattcatcat ccaaatcacc accctttata gttacgatga aggctttatc
    14101 ttttaccaat ggcagtgaat atcggaataa aattattatt ggcacaacag cacggctaac
    14161 aattaaatca aacgaacctt tgtacttctc aataaattca ggactttcaa cacgggcatt
    14221 ttcagcaact gcattactta atttaagttt atctaaaaat tctttaactg catcaatttt
    14281 ttttccagtt gaatccacaa gtacaccccg taaatccgga cgcataattg ctagtggaat
    14341 tcccggaaag ccgccgccgg tgccaatatc taaaaatcgt gttacttttt ctggtaaata
    14401 ttttgttacg taggcagaaa taaagatatg attttcgata atgctatcaa tatcttttct
    14461 ggaaattaaa tttactgttt tatttttttc agcaacaagc tgtgcaaaaa atgcgagtct
    14521 ttcattgcgc attggctcgg gattaaaacc attatcccaa caaaaacttt gtaattcttt
    14581 tagataatta tcttgtttgg taggcggcat taaaaaatcc tgcaaattag tttttcaaat
    14641 ataccaacaa aatggaaata tctgaaggtg ttacacctga gattcttgat gcctgagcaa
    14701 tagatcgcgg tttaactttg ttcagttttt ctttcccctc tgttgataat tgtttgattt
    14761 ttgagaaatc atagtccaat ggaatttttg tgctttcata tttttcaagc ttgccagcag
    14821 cttcgtgctg tcgtaaaata taaccttcat attttatttc tatttcggcc tgttcgagcg
    14881 ccttttcatc ttgcagcaaa gcaactgcct ccagatcatt agcagttcca ttaatctcca
    14941 gtaattgttt tagattaaat tcgggccttt tacaaagttt tgatattgtt tctgtcgaat
    15001 caatttcatt tgaacctgag tttattagga attgatttat atctctagcg tgatattttt
    15061 tagtatgaca atagttgatg ccatcgatta ttgttttttc tcgattatca acattatcat
    15121 acaaattttt gggaattagc ccaaattcat aaccatattt catcaatctt ctatccgcat
    15181 tatcttgtct aagcaatagt ctatgctctg ctctggatgt aaacattctg taaggttcat
    15241 ctgtagattt tgttactaaa tcatcaatta aaactcctat gtatgcctca cttcttttta
    15301 aaacaaattc tggtttaccc tgaattttta aagctgcatt tattccagca actaatccct
    15361 gtgcagcggc ctcctcatat ccagaagttc cattaatttg tcccgcaaag taaagtcctt
    15421 tgatcaactt agtttccaaa gtcagatcaa cttgatgagg tggaaagaaa tcatactcaa
    15481 cagcataacc tggacgtacc atttcaacat tttctaatcc aacaatttta tgcagtgctt
    15541 caagctgaat atcagcatca agtgaagatg aaaagccatt caaataaatt aaatcagagt
    15601 ctaaaccttc aggctctaaa aagagctgat gtctttcctt atcagaaaac ctaactatct
    15661 tatcttcaat tgatggacaa tatcttggtc caactccctt tatcaatccg gtaaacaatg
    15721 gcgaagctgc aaaacctttt tcaagaattt tatgcactaa tggatcggta taagtgatat
    15781 gacaacttac ttgaggaaga aaaggaaaat tagacatatc tgtttgatga gaaaatggtt
    15841 gtggattttc atccccaggt tgctcttcca aaatttccca attaattgaa tcttttttta
    15901 atcttggtgg ggttcccgtt tttaatctac catagtcaaa tccaagctta gctaaagatt
    15961 cagtaatccc cagagccggt tgttcaccaa atctaccgcc actggtttta ttcaaacccg
    16021 tgtgcatcaa cccatttaaa aaagttccgg atgaaaggat caatgcttta cacaatattt
    16081 cctctccgct ttgagttttt attccaacta cttttccatc ttcagcaatt acatcaacag
    16141 cggattcctc tacaatctca agattttgag tttgagaaat ttttctataa gcttcttcgg
    16201 agtataattt tctatccgat tgacaacgcg gagaccaaac ggctggacct tttgatttat
    16261 ttaacatcct gaattgtata cctgtttgat ctgcaatttg acccattact ccgcctaatg
    16321 catcgatctc gcgtactaaa tgtccttttg ctgttccacc aattgctgga ttacaggaca
    16381 ttcttcctag tgcatatcta tccattgtga ctaaaccaac ggaacagccc attctggctc
    16441 cagcagaggc tgcttctatt ccggcatgtc caccaccaac tacaataaca tcaaatttct
    16501 tcattttata atcttttttg ttttcacgtg aaacaagatt ttttgttaaa tgctaattat
    16561 ttccgtttca cgtgaaactg aaccattact tccctatgca aaatttcata aaaatattgt
    16621 ttaaaatgtc gtccgaagta acttcaccta taatctcagc aagattcatt tctgcatttc
    16681 ttaaatccac agaaataaat tcaccgctca ttttctttaa tattgattct cttgcattta
    16741 ttaaattttc tctagatttt ttcagacagt tgtgatgtct taagttcgtt actattgcag
    16801 ttttttctgt ataactctca tttcccactg tcttctcttt taactttcca aacaacgctt
    16861 caattccctc acccgttttg gcagaaattt ttatatctgc ttctttcgaa atgtttttat
    16921 ccaaatcaat tttatttatt gccttaataa tcttatcgtg acttgtaaaa tttaataaat
    16981 ccacaaaaag atctttagat tcctcttgct gaacatcttc taaataaatt acaatatctg
    17041 cacttctta
//
LOCUS       DASWBY010000684         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_553136, whole genome shotgun sequence.
ACCESSION   DASWBY010000684 DASWBY010000000
VERSION     DASWBY010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553136"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VF870_12520"
     CDS             <1..777
                     /locus_tag="VF870_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX9253062.1"
                     /translation="IISNDIVINEFMADNAATITDPSGEYDDWIELYNPTSDPIVLTG
                     RYLTDKKDNLTKYQFTQDNLILNPNEYLLIWCDEQGSQQGIHTNFKLSKDGEFIGLVD
                     SDGISIIDSITFGPQTTDISFGRYPDGANEWVFMSPTPGATNNIVSVDDEEIIPTEFS
                     LSAYPNPFNPSTTIRFSTPPASSPLVKGRNEVGFVTLRIYDILGNEIATLVNEEKPPS
                     TYEVLWNGENNLGSKVSSGIYFVRIQANQQFKNLKLMLLK"
     gene            1138..>2087
                     /locus_tag="VF870_12525"
     CDS             1138..>2087
                     /locus_tag="VF870_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9253063.1"
                     /translation="MARKNVLIMGAAGRDFHNFNVFFRDNKDYNVVAFTATQIPNIEG
                     RTYPKQLAGTLYPNGIKIYEEKELETLIKKLKVDEVVFSYSDVPFEYVMTKASIVNAC
                     GVSFRLLGAAETEVKSTKPVIAVLAVRTGCGKSQTSRKIVEVLTAAGKKVVAIRHPMP
                     YGDLVKQKVQRFATYEDLKKHKCTIEEIEEYEPHVARGGVIYAGVDYEAILREAEKEA
                     DVILWDGGNNDFSFYKADVTFTVVDPHRPGHEMKYYPGNTSLRLADAAVINKIDSADN
                     AGINTVRDNIRKVNPNATIIEAASPVTVEHPEMIRGKRVLV"
BASE COUNT          718 a          350 c          385 g          634 t
ORIGIN      
        1 attatttcta atgatattgt tataaatgaa tttatggctg ataacgcagc aacaataact
       61 gatccttctg gagaatacga tgattggatt gaactttata atccaacatc agatccgatt
      121 gttttaaccg gaagatattt aactgataaa aaagataatc taacaaagta ccaattcact
      181 caggataact taattttaaa tccaaatgag tatctattaa tttggtgcga tgaacaaggt
      241 tctcagcaag gtattcatac aaatttcaaa cttagtaagg atggtgagtt tatcggttta
      301 gttgattcag atggaatttc tatcatagac tcaataacat tcggtccaca gacaactgat
      361 atttcttttg gcagatatcc ggatggagca aatgaatggg tatttatgtc cccaactcca
      421 ggtgcaacaa ataatattgt ttcagttgat gacgaagaga ttatacccac tgaatttagt
      481 ttgagtgcgt atccaaatcc atttaatcct tctacgacta tcagattttc aacccctcct
      541 gcctcctccc ctttggtaaa ggggaggaac gaggtggggt tcgtgacttt gagaatttat
      601 gatatactcg gaaatgaaat agcaacatta gtaaacgaag aaaaaccacc cagtacttat
      661 gaggttttat ggaatggaga aaataattta ggaagtaaag tcagttcagg aatttatttt
      721 gttagaattc aggctaatca acaatttaaa aatttaaagt tgatgttatt gaaataatgc
      781 tatgtaagaa cagtgctcgc cctgtccgcc tgatttataa taactttgcg agtaaaaata
      841 tttcggggag aggcaaacct ctcccctact tttatgtttt ctttagcttt ttacttttta
      901 tgttaaataa ataaaaagat tttttttgat cgcacatcta attttcttgc cctatttaac
      961 ttaaacgcac tttttaaatt acttacattg taggaccaat taaagcgacc gatttttcga
     1021 ggaaacttgg acaaaatccc ttaaatttgg atcaataaat ttttaaatac taataaaaat
     1081 gaatcaaaat atttttatac aacaacaaaa tctaaaagca caaaggagag tatttctatg
     1141 gctcgtaaga acgtgttgat aatgggcgct gctggacgcg atttccacaa ctttaatgtt
     1201 ttcttcagag acaacaaaga ctacaatgtt gtagcattta ccgctactca aatcccaaat
     1261 attgaaggcc gcacttaccc caagcaactt gccggcacac tttatccaaa cggaataaag
     1321 atctacgaag aaaaagaatt agaaaccctt attaaaaaat taaaagttga tgaagtagtt
     1381 ttctcatact cagatgtacc atttgaatat gtcatgacaa aggcttcaat cgttaatgcg
     1441 tgcggagttt ctttccgatt actcggcgct gctgaaactg aagttaaaag tactaaacct
     1501 gttattgctg ttttagctgt tagaaccggc tgcggtaaat cgcagacatc aagaaaaatt
     1561 gttgaagtat taactgctgc cggtaaaaaa gttgtagcaa ttcgtcatcc aatgccttat
     1621 ggtgatcttg taaaacaaaa agtacagcgc tttgcaactt atgaggatct aaaaaaacat
     1681 aaatgtacaa tcgaagaaat tgaagagtac gaaccacacg ttgcaagagg cggagttatt
     1741 tatgcaggag tagattacga agcaatctta cgcgaagcag aaaaggaagc tgatgtaatt
     1801 ttatgggatg gtggcaataa tgatttctca ttctacaaag ctgatgtaac tttcacagtt
     1861 gttgatccgc atagacctgg acacgaaatg aaatattatc ccggcaatac ttctttaaga
     1921 ttggcagatg cagcagttat taataaaatt gattcagctg ataatgctgg tataaatact
     1981 gtaagagata atatcagaaa agttaatcct aacgcaacca taattgaggc ggcttcaccg
     2041 gtgacagttg agcatccgga aatgattcgt ggcaaaagag tattggt
//
LOCUS       DASWBY010000685         4406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_515491, whole genome shotgun sequence.
ACCESSION   DASWBY010000685 DASWBY010000000
VERSION     DASWBY010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515491"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VF870_12530"
     CDS             <1..621
                     /locus_tag="VF870_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX9253064.1"
                     /translation="REYEVYDPAIDKSLIIGEDYIMSPQDLCTIEFIDQLIEAGIDSF
                     KIEGRKRAPEYVAKAVSVYRQAIDLYFEDNLTKEKKKELLTELEKVYNRGFSTGFYFG
                     IPSSKEYAGVEGSKATTRKVYVGKVLNYYKEIEVAHILMESEQIKIDDEILIMGETTG
                     VFEIKLEKLTVNDKEESIAGRGDEVTLKVPSLVRRNDKVYLIEYVK"
     gene            675..977
                     /locus_tag="VF870_12535"
     CDS             675..977
                     /locus_tag="VF870_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX9253065.1"
                     /translation="MIKKEGKKMNCPVCSNTSLVISERQGVEIDYCPKCRGVWLDRGE
                     LDKIIERTNEVYDNEKPQNYEDRNYENKKHYFDKKYDDDYYKRNKRKSFFNDLFDF"
     gene            981..1934
                     /locus_tag="VF870_12540"
     CDS             981..1934
                     /locus_tag="VF870_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX9253066.1"
                     /translation="MTGIEIILYLVGGLIMLFIGAEGLIRGSSNLALKIGITPLVVGL
                     TVVAFGTSTPELVVSLKAALLGNSSISLGNVVGSNIANIALILGVAALIRPLDVHANV
                     IRREIPIMIVISLLLLLILIDGEIGFIDGLMFVIGLIVYLIINVIMARKEKNPEVDAE
                     FKEGLKSKIGIPVSIVLMIAGLGLLILGANLFVQSAVAIAKIFNVSDAIIGLTIVAVG
                     TSLPELITSIVASYKKEADIAIGNVVGSNIFNILGILGITAIIIPISSVGISYFDLGV
                     MLFAAIILLPLSRTGFSISRLEGALLLAGYAIYIYYLLPTQ"
     gene            complement(1941..2495)
                     /locus_tag="VF870_12545"
     CDS             complement(1941..2495)
                     /locus_tag="VF870_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX9253067.1"
                     /translation="MYTKPFHPWHNVSPGKDIPKTVNGIIEIPKGSKGKYELDKESGL
                     LRLDRVLYSSVHYPANYGFIPQTYCDDKDPLDILIICSIDVDPLCLIEAKVIGVMHML
                     DDDEQDDKIVAVANNDMAVNYINDISELPPHTVVELRRFFEDYKKLEHKQVIVEQFLG
                     KEDAYKIINEAVELYSKEKENLIK"
     gene            complement(2510..3811)
                     /locus_tag="VF870_12550"
     CDS             complement(2510..3811)
                     /locus_tag="VF870_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX9253068.1"
                     /translation="MEHASEVEIPLLTLLPFVLMLLSIAIFPLFWNHFWEKNKNKLLV
                     AIVLSIPVVIYLLMNGFSHELLLTMVFDYVPFLILLGALFTITGGIFLTGDIEAKPSI
                     NTLFLGIGAVLASVMGTTGAAMLLIRPVIQTNKERTFKVHTILFFIGIVANCGGLLTP
                     LGDPPLFMMYLRGAHFTWFLHLIPEWFITNALLLIVYFFVDTYYHKKEPASAIQRDET
                     NIRPIKIQGTQNFIFLIGVVLAVAFINEQYLSFIKINPYFKFIREAVIVLMAFLSMQF
                     TPKLLRTSNNFTWHPIQEVAYLFLGIFVTMVPALLYLEVNAKHLGVQTVGQFYYYTGL
                     LSSFLDNTPTAVTFHSLALGLGITEGTLVAGIPEILLKAISVGAVFFGSMTYIGNGPN
                     FMVKAVAEENNIKMPDFFSYMFKFSIIVLLPIFILVQLLFI"
     gene            4005..>4406
                     /locus_tag="VF870_12555"
     CDS             4005..>4406
                     /locus_tag="VF870_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9253069.1"
                     /translation="MRLSKYFVPTLKEVPNDAVVVSHILMLRSGMVRMLSAGIYSFLP
                     LGYKVMRKVSEIIREEMDAIGGQEFHLPALNPTEIWEETNRVEAFGDTMFHLKNREYV
                     LAPTHEEIMTFHARNVVKSYKDMPQIWYQIQT"
BASE COUNT         1548 a          672 c          785 g         1401 t
ORIGIN      
        1 agagaatacg aagtttatga tcctgcaatt gataaatctt taattattgg cgaagattat
       61 ataatgtctc cgcaggactt atgtactatt gaatttattg atcagttaat tgaagctgga
      121 attgattcat ttaaaataga aggaagaaag cgcgctccgg aatatgtagc gaaagcagta
      181 tcagtttatc ggcaggcaat tgatttgtac ttcgaggata atcttaccaa agaaaagaaa
      241 aaagaattat taaccgaact tgaaaaagta tacaatcgtg gattttcaac cggattttat
      301 tttggtatac caagttcaaa agaatatgca ggagttgagg gcagcaaagc aacaacgcga
      361 aaagtttatg tcggtaaagt tcttaattac tataaggaaa ttgaggtcgc acatatttta
      421 atggaatcag aacagattaa aattgacgat gaaattttaa ttatgggtga aactacagga
      481 gtatttgaga tcaaattaga aaagctgact gtaaatgata aagaagaatc aattgcaggc
      541 agaggcgatg aagttacttt aaaagttcca tctctggtca gaagaaatga taaagtttat
      601 ttaattgagt atgtaaaata atgctggtgt tcaaattctt ctaaaatgaa cttttttaat
      661 catcatttag ttttatgata aaaaaggaag gaaagaaaat gaattgcccg gtttgctcaa
      721 atactagttt agttatttct gaaagacaag gtgttgaaat agattattgt cctaaatgcc
      781 gcggtgtttg gcttgaccgc ggtgaacttg ataaaattat cgaacgaaca aatgaagttt
      841 atgataatga gaaaccacaa aattatgaag acagaaatta cgagaataaa aagcactatt
      901 ttgacaagaa atatgacgat gattactaca aaagaaataa aagaaaatcc ttttttaatg
      961 acttatttga tttttaattt atgacgggaa ttgaaataat cttatatctg gttggcggac
     1021 tgataatgct tttcataggt gcggaaggtt taattagagg cagttcaaat cttgcactta
     1081 aaattggaat tactccgctt gtggtaggat taacagttgt ggcttttgga acaagcacac
     1141 ccgaattggt tgtaagtctt aaagctgcac ttcttggtaa cagctcaatt tctttaggta
     1201 atgtcgtggg ttctaacatt gctaatattg cgcttatcct tggtgttgcg gcgttgataa
     1261 gaccacttga tgtacatgca aacgtcataa ggagagaaat accaataatg atcgtgataa
     1321 gcttattgct tcttctcatt ttaattgatg gtgagattgg gtttattgat gggttgatgt
     1381 ttgtaattgg attaatcgtc tatttgataa ttaatgttat catggcgcgt aaagaaaaaa
     1441 atccagaagt tgatgctgaa tttaaagaag gcttaaagag taagattgga attcctgttt
     1501 ctattgtttt aatgatagca ggattaggat tgttaattct cggggctaat ctattcgtgc
     1561 aaagtgctgt tgcaattgct aagatattta atgttagtga tgctattata gggttaacaa
     1621 ttgttgcagt aggtacgagt cttcctgaat taattacttc aatagttgct tcttataaaa
     1681 aagaagctga tatagctatc ggaaatgtag tcggatcaaa tatttttaat attctgggaa
     1741 ttttgggaat aacagctatc ataattccca ttagttctgt aggaataagt tattttgatt
     1801 taggtgtgat gttatttgct gctataattt tgcttccgct tagcagaact ggattttcaa
     1861 tctctaggct ggaaggggct cttcttcttg ccggatatgc catttatatt tactacttac
     1921 tgcccaccca ataataaaaa ttacttaata agattttctt tctctttaga ataaagttca
     1981 actgcttcat ttataatttt atacgcatct tctttgccaa gaaactgctc aacaattacc
     2041 tgtttatgtt ctaacttttt ataatcttca aaaaatcgtc ttagctctac aactgtatga
     2101 ggcggtaatt cagaaatatc attaatgtag tttactgcca tatcattatt tgcaacagca
     2161 acaattttat catcctgctc atcatcatca agcatatgca ttacaccaat aacctttgct
     2221 tcaataagac aaagtggatc aacatcaata gagcatatta ttaaaatatc aagcggatct
     2281 ttatcatcac aataagtttg tggaataaat ccatagttag caggataatg aactgatgaa
     2341 taaagcacgc gatctaatct taataaaccg ctttccttat caagctcata cttgcctttt
     2401 gatcctttag ggatttctat aattccattt actgtttttg gtatatcttt accgggacta
     2461 acattatgcc agggatgaaa tggttttgtg tacatcgatc actcttttct tatataaata
     2521 acaattgaac taaaataaaa atcggtaaaa gaactataat cgaaaactta aacatatagc
     2581 tgaaaaaatc aggcatcttg atattatttt cttccgctac agctttaacc ataaagtttg
     2641 gtccgttacc aatgtacgtc attgagccaa agaaaacagc acccactgaa attgctttta
     2701 gaagaatttc cggtattcct gcaacaagcg ttccttctgt tattccaaga ccgagcgcca
     2761 gggaatgaaa tgtaactgct gttggagtat tgtcaagaaa gctgcttaac aatcctgtgt
     2821 aataataaaa ctgaccaaca gtttgaactc ccaagtgttt tgcattcact tcaagataaa
     2881 gtaaagcagg aaccattgta acaaaaatac ctaaaaataa ataagcaact tcttgaatcg
     2941 gatgccaggt aaagttgttt gatgtgcgta acagttttgg agtaaattgc attgataaaa
     3001 atgccattaa aacaattaca gcttctctta taaatttgaa gtagggatta attttaataa
     3061 atgataaata ttgttcatta ataaacgcca cagccaaaac aaccccaatc aagaaaataa
     3121 aattttgtgt cccctgaatt tttatagggc gaatatttgt ttcatctcgt tgtattgcac
     3181 tagccggttc ttttttgtga taataagtat caacaaagaa ataaacaatt aataaaagtg
     3241 cattcgtgat aaaccattct ggaattaaat gtaaaaacca tgtaaaatga gcaccgcgta
     3301 aatacatcat aaaaagtggg ggatcaccta gaggtgttaa taatccacca cagtttgcaa
     3361 ctattccaat aaaaaataaa atagtatgaa ctttaaatgt tctttcttta ttagtttgaa
     3421 taaccggacg aattaataac attgctgcac ctgttgttcc catcactgac gcaagcacag
     3481 ctccgattcc caaaaacagg gtattgattg atggcttggc ttcaatatct ccggttaaaa
     3541 atataccacc ggtaatagtg aatagggctc caagtagaat taaaaatggt acataatcaa
     3601 aaaccatagt aagtagtaat tcatgtgaaa agccattcat aagaagataa attacaacag
     3661 gaatactaag aacaattgcc actaagagtt tgtttttatt cttttcccaa aagtgattcc
     3721 agaacaaagg aaaaatagcg atggataaaa gcattaaaac aaatggtagt agtgttaaaa
     3781 gcggtatttc aacttccgat gcgtgttcca tagataacct tcagtgattg cataaatgtt
     3841 aaaaattcag gaagaaagct aaagaaatta ttatcaattt tacatctata taacacagat
     3901 tttagaacga taacttagac ttaatcagct ttgataagca tcatcaaatc agtattttta
     3961 gcctcaaaat ttcacatcaa acaataaaaa tcagaaaagt atatatgaga ttaagtaaat
     4021 attttgtccc aacattaaaa gaagttccga atgatgccgt tgttgtaagc catattttaa
     4081 tgcttcgttc cggaatggtt agaatgttat ctgcgggaat ttactcattt cttcctcttg
     4141 gatataaagt tatgagaaaa gtaagtgaaa ttatccgcga agaaatggat gcaataggcg
     4201 gacaggaatt tcatctaccg gcgcttaatc caacagaaat ctgggaagaa acaaatcgag
     4261 ttgaagcttt tggtgatacc atgtttcatt taaaaaatcg cgaatacgtt ttagctccaa
     4321 ctcacgagga gataatgaca ttccacgcac gaaatgttgt aaaatcatat aaagatatgc
     4381 cgcaaatctg gtatcaaata cagact
//
LOCUS       DASWBY010000686         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_515497, whole genome shotgun sequence.
ACCESSION   DASWBY010000686 DASWBY010000000
VERSION     DASWBY010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515497"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="VF870_12560"
     CDS             <1..1565
                     /locus_tag="VF870_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX9253070.1"
                     /translation="IGSSLGSVVGQFLRVSPKRLKTLVGCGAAAGIAAAFNAPIAGAL
                     FAVEIVLMDFAVAQFSPIVISSVMATVISHTFEGNFTAFIVPKYQLASPVEIGFYFVL
                     GAASGIVAYLFIKTLYYCEEIFDNSINIPEYLKPALGGIGVGIIALMFPEIMGVGYDS
                     INMALTGNLFWYFALALIFMKIIATSLTLGSGGSGGIFAPSLFMGAMLGYFFGTIVHT
                     YFPNITASPGAYALVAMGGLVAGTTRAPITAIIIVFELTNDYRIILPLMITCVMSMVI
                     SSKLSRESIYTLKLVLRNIGLKEGMETNIMESIYVKNVYRKDYESISFADNFSQIVNR
                     IIRGKETDFPVVNKKGVVTGMISIHDVKDYLYEKDSLENILIAGDLANTYFETLCPDD
                     SCQTALDKFRKFSFNGLPVVENQETRKLIGIIWRKDIQDAYDKEIEKRELTSNLASLI
                     KMKEEEPMVHFMEGYSIIEIAPPPSFIGKSIRQLNIRSAYGVDVLSIKTKEKKGEKIM
                     SQSALPITSAKL"
BASE COUNT          526 a          246 c          328 g          520 t
ORIGIN      
        1 agataggttc aagtcttggt tccgtcgttg gacagtttct gagagtctct ccgaaaagat
       61 taaaaacact agttggatgc ggcgcagcag ctggaattgc tgcagcattc aatgcgccaa
      121 tagcaggagc tttatttgct gtagaaattg tgttgatgga ctttgctgtt gcgcagtttt
      181 cacccatcgt tatttcatct gtgatggcaa cggtaatatc acataccttt gaaggaaact
      241 ttacggcttt tatagttcct aaatatcaac ttgcctctcc ggtggaaatt ggattttatt
      301 ttgtacttgg tgcggcaagt ggaatagttg catatctttt tattaaaaca ctttattact
      361 gcgaagagat ttttgataat agtattaata ttccagaata tttaaaacca gcgcttggcg
      421 gaataggtgt tgggataata gctttaatgt ttcctgaaat tatgggagtt ggatatgatt
      481 caattaatat ggcacttacc ggaaatttgt tttggtattt tgcattagca ttaattttta
      541 tgaaaattat cgcaacttct ctaacgcttg gttccggtgg ttccggggga atatttgctc
      601 cttcactttt tatgggtgcg atgctgggat atttttttgg aactattgtt cacacttatt
      661 ttcccaatat aaccgcatct cccggagctt atgcacttgt tgcgatggga ggtcttgtag
      721 caggaacaac aagagctcct ataactgcaa ttattattgt ttttgaacta acaaacgatt
      781 accgcattat tctaccattg atgataactt gtgtaatgag catggtcata tcttccaagc
      841 tttcaagaga atcaatctat actttaaaac tggttctaag aaatatcggt ctgaaagaag
      901 gtatggaaac caacataatg gaatcaatct atgttaaaaa tgtttacaga aaagattatg
      961 agtcaatcag ctttgccgat aacttcagcc aaattgtaaa tagaattata aggggaaaag
     1021 aaacagattt tcctgtggtt aataaaaagg gagttgtaac cggaatgatt tccatccacg
     1081 acgtaaaaga ttatttatac gaaaaagatt cgttagaaaa tattcttatc gccggagatt
     1141 tagcaaatac atatttcgaa acactttgtc ccgatgatag ttgtcaaact gctttagata
     1201 agttcaggaa attttcattt aatggtttgc cggtggttga aaatcaggaa acgagaaaat
     1261 taataggtat tatctggaga aaagacattc aggatgctta tgataaagaa atagagaaaa
     1321 gagaattaac atcaaacctt gcaagtctga ttaagatgaa agaagaagaa ccgatggttc
     1381 attttatgga aggttattca attattgaaa ttgcacctcc cccctctttt attggcaaat
     1441 caataagaca gttaaatatt agaagcgctt acggtgttga tgttttatcg attaaaacca
     1501 aagaaaaaaa aggagaaaag attatgagtc aatcagcttt gccgataact tcagccaaat
     1561 tgtaaataga attataaggg gaaaagaaac agattttcct gtggttaata aaaagggagt
//
LOCUS       DASWBY010000687         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_255373, whole genome shotgun sequence.
ACCESSION   DASWBY010000687 DASWBY010000000
VERSION     DASWBY010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255373"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VF870_12565"
     CDS             complement(<1..208)
                     /locus_tag="VF870_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253071.1"
                     /translation="MKLLCTIIIICATTFSAYSQYNPGAKQISLSNSDVALSNDVFSL
                     FNNPAGLSQMNWREVGAYYSPAPFG"
     gene            278..1156
                     /locus_tag="VF870_12570"
     CDS             278..1156
                     /locus_tag="VF870_12570"
                     /inference="COORDINATES: protein motif:HMM:NF027445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HEX9253072.1"
                     /translation="MIKHIFLILVLIPFTLSFTQELNCRVTVNYEGLSVENRERLIDF
                     SGVIESYMNSTQFTGQAWKGQKIDCSLTIFFTSAAGDDYSAQVVVVSTRPVFRSTRQS
                     PMLTISDPTWSFRYVQNQAMYANQSTFDPITTFLDFYANIIIGFDWETWAELGGTPYF
                     NKAFNLLNLSVGSTYKKGWEQSSAAYSRWGLCQDLLNDNYRAFREAFYVYHHGVDEIQ
                     LRKKDGQDKIAAIVSVFEDMQKKRGINSVLVKLFFDTKYGEIIELLKDYPDNMIFSRL
                     KKVDPPHSAKYEEMSH"
     gene            complement(1165..>2488)
                     /locus_tag="VF870_12575"
     CDS             complement(1165..>2488)
                     /locus_tag="VF870_12575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9253073.1"
                     /translation="NSEINLDRVKPIFWEEFDRSNILLRIQFSKILHNLRFISKSFPD
                     LNIDYYFRLLDDSNPNVALQTAISIKNIKNYLNNTLNISVKNKIDSLLFEVRKSMNFK
                     GELLLSRYDLFGGYEEHEIILNELKLPAKYQIQFYGKHPDTEIAFNKLSDFYWNSSEL
                     KTRLESLTQILELKSDPKYLNEEFKLLLNSLNSDQAPLISVAADGIDSALITKEADKL
                     KQIISEQIINYEDNPDFLEATMSLANLSEKMDKDFYKSIVETASSSKLYSIRKFIANK
                     TANYQIGFKELDMFESIWQNVFKFKQATIKTSKGNIVIKFDSEIAPISVANFCMLVKK
                     DFYNGIIFHRVVPGFVIQAGDPTATGWGGPGYDIVSEFSDTDFGIGYVGMASAGKDTE
                     SSQFFIMQGSHPHLDSRYTLFAKVIEGMDVVYNITEDDKILDIQIEQN"
BASE COUNT          799 a          398 c          427 g          864 t
ORIGIN      
        1 atccaaacgg ggcaggagaa taataagccc caacttctcg ccagttcatt tgtgataatc
       61 ctgctggatt attaaaaaga ctgaaaacat cgttgcttaa agcaacatct gaattggaga
      121 gggagatttg ttttgctccg ggattatact gagaatatgc actaaaagtt gttgcgcaaa
      181 ttattataat tgtacaaaga agtttcataa tatttgatag ttatttgttc cattatatct
      241 aaattttaaa attaaatcaa caaaaatttt ggctaagatg attaagcata tttttttaat
      301 actggttcta attccgttca cactttcttt cacgcaggaa ttaaattgca gagttacagt
      361 taattatgaa ggtttgtccg ttgagaacag agaaaggctt atcgatttta gcggcgttat
      421 tgaatcttat atgaattcaa ctcaatttac cgggcaggcg tggaaaggtc agaaaataga
      481 ttgttcatta actatatttt tcactagtgc cgcaggtgat gattattctg cacaagttgt
      541 tgttgtatca accagacccg ttttcagatc cactcgtcaa tctcctatgc taactataag
      601 tgatccgacc tggtcattca gatatgttca aaatcaggct atgtatgcta atcaatctac
      661 ctttgatccg ataactactt ttttagattt ttatgcaaat attattattg gattcgattg
      721 ggagacatgg gcagaattag gcggcacacc ttactttaat aaagctttta atttgttaaa
      781 tcttagtgtc ggaagtacct acaagaaggg ctgggaacag agcagtgctg catacagcag
      841 atggggttta tgtcaggatt tgcttaatga taattaccgt gcatttagag aagcatttta
      901 tgtttatcat cacggtgtag atgaaataca attaagaaaa aaagacgggc aggataaaat
      961 tgcagctatt gttagcgtgt ttgaggatat gcagaaaaaa agaggaatca atagtgttct
     1021 tgttaaactt ttttttgata caaagtatgg agaaataata gagttgctga aagactatcc
     1081 cgataacatg atattcagta gattgaagaa ggtagaccca ccgcattctg ctaaatatga
     1141 agaaatgtcg cactaagaat atttttagtt ctgttcaatt tgtatgtcga gtattttatc
     1201 atcttcggtg atgttataaa caacatccat cccttctata acttttgcaa ataatgtgta
     1261 tctgctatcc agatgtggat ggctgccctg cattataaaa aattgagaac tttctgtgtc
     1321 tttcccggca cttgccattc ccacataacc aattccaaaa tcagtatcag aaaattcaga
     1381 gacaatatca taacctggtc cgccccagcc tgtagctgtc ggatcaccag cctgaataac
     1441 aaacccggga actacacgat gaaaaattat tccattataa aaatcttttt taactaacat
     1501 gcaaaaattt gcaactgata ttggggcaat ttctgaatca aattttataa caatatttcc
     1561 tttggaagtt ttaatagttg cttgtttaaa cttgaaaacg ttttgccaaa tactctcaaa
     1621 catatcaagc tctttaaatc cgatctgata attcgcggtt ttatttgcaa taaattttct
     1681 gattgagtac agtttagaag aacttgccgt ttcaacaatc gatttataaa aatctttatc
     1741 catcttttca gagagatttg ccagtgacat ggtggcttcg agaaaatccg gattgtcttc
     1801 gtaatttatt atttgttcgg atataatttg ttttagcttg tctgcttctt ttgtaattaa
     1861 agctgaatca attccatcag cagccactga tataaggggc gcctgatcgg agttaagcga
     1921 gtttaataat aatttaaatt cttcatttaa atattttgga tctgatttta attccaatat
     1981 ttgggttaag gattctaatc tggtttttag ttctgatgaa ttccagtaga aatcacttag
     2041 tttattaaaa gcaatttcag tgtctggatg ctttccataa aattggattt gatatttggc
     2101 gggtagtttc aattcattca agataatttc atgttcttca taaccaccaa acaaatcata
     2161 tcgactcaat aacaattctc ctttgaaatt catagatttc ctcacctcaa atagcagaga
     2221 atcgatttta tttttaactg aaatatttaa agtgttattc aaataatttt tgatattctt
     2281 tatagaaata gctgtttgta atgcaacatt gggatttgaa tcatctaata gtctgaaata
     2341 gtaatctatg tttaaatctg gaaatgattt cgaaatgaat cttaaattat gtaagatttt
     2401 cgagaattga attcgtaaca aaatattact ccggtcaaac tcttcccaaa atattggttt
     2461 tactctatca agatttattt cactgtta
//
LOCUS       DASWBY010000688         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_291800, whole genome shotgun sequence.
ACCESSION   DASWBY010000688 DASWBY010000000
VERSION     DASWBY010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291800"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VF870_12580"
     CDS             <1..309
                     /locus_tag="VF870_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9253074.1"
                     /translation="MDKKKISVLVFPEGTRSKTGEVQSFKRGAFYLAAHSGYPIVPIT
                     INGTKKIMPKGTFKLKRGTIYLHFDKPIPTDKIKSKPDELALMEKVREIIISNQKEII
                     "
     gene            309..602
                     /gene="gatC"
                     /locus_tag="VF870_12585"
     CDS             309..602
                     /gene="gatC"
                     /locus_tag="VF870_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX9253075.1"
                     /translation="MAVTKNDVEKIVELARLKFSEEELQNFTPQMNEILSYMNKLNEL
                     NTENVEPLSHPVEQTNVFREDEMRPSIPTEAALKNAPAKDERHFKVPKVIGDK"
     gene            602..1405
                     /gene="kdsB"
                     /locus_tag="VF870_12590"
     CDS             602..1405
                     /gene="kdsB"
                     /locus_tag="VF870_12590"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX9253076.1"
                     /translation="MAKKVKPKKNNPYILGVIPARFASTRLMGKPLADIGGKFLLQHT
                     FEGASKSKLLNKIIIAVDDDQVAKAAKGFGASVIMTPKDCPTGSDRIAIVAKEFPRAD
                     IIVNIQGDEPFISGMMIDQAIEPLLFDEEVNVSTLAKLVEDIEEFKSPSVVKVVFDYK
                     NFALYFSRSPIPFVRDAKDEKEMIEKANIYKHIGLYVYRKEYLLKFTKLEPTDLEQWE
                     KLEQLRMLENGFKVKIVETEFDSFSVDTPEDLEKARKIYSKLKKIELAE"
     gene            1402..2361
                     /locus_tag="VF870_12595"
     CDS             1402..2361
                     /locus_tag="VF870_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9253077.1"
                     /translation="MITPQKISLAQIPTPLEEIKFEGKSFLIKRDDLTGCELSGNKVR
                     KLEYLLAHAKLKKADIIFTPGGDQSNHARATVIAARKIGLKTKLFLWGKETPYPNGNL
                     FLDKAFGADIQYFTKAEYEKVNEIMFEQRLALLKKKMNAYVFPAGGSTTLGIWGYINF
                     INELKNQVDLKKIDSIVAACGSGGTAAGMLVGAALNNLTLKIVAVHVLMTKKEMEKHI
                     LQLAEGCVLDYKLKCKINPENLIVMDGYSNEGYKKISASKVKLIKKFAQETGILFDPA
                     YTGKAFTAYYERYLNTGRGKKNIFVHTGGLFGVFGRTKEYLSN"
BASE COUNT          954 a          381 c          493 g          763 t
ORIGIN      
        1 atggataaga aaaaaatatc tgttctggtt tttcctgaag gaacaagaag caaaaccgga
       61 gaagtacaaa gttttaaacg tggcgcattt tatcttgctg cacatagcgg ctatcctata
      121 gttccgataa ctataaatgg aacaaaaaaa attatgccta aaggaacttt taagctgaag
      181 aggggaacaa tctatttgca ttttgataaa ccaattccaa ctgataaaat aaagtctaaa
      241 ccagatgaac ttgcgttaat ggaaaaagtt agagagatta ttattagtaa tcagaaagag
      301 ataatttaat ggcagttaca aaaaatgatg tagagaaaat tgttgagctt gcaagattaa
      361 aattttctga agaagagctt caaaatttta ctccacagat gaatgaaata ttatcctata
      421 tgaataaatt aaatgaactt aatactgaaa atgttgagcc gctttcgcat ccggtagaac
      481 aaacaaatgt ttttagagaa gatgaaatga gaccttctat tcccactgaa gcagctttaa
      541 aaaatgctcc ggcaaaagac gaacgccact ttaaagttcc taaagttatc ggtgataaat
      601 aatggcaaag aaggttaagc caaaaaaaaa taatccttac attcttggtg tcattccagc
      661 cagatttgca tcaacaagat tgatgggcaa accgctggct gatataggcg gaaaatttct
      721 tcttcagcat accttcgaag gggcaagcaa atcaaaactt ttgaataaga taattattgc
      781 tgttgatgat gatcaggttg caaaagctgc aaagggattt ggtgcttcag taataatgac
      841 tccaaaagat tgccctacag gatcggatag aatcgcaatt gttgcaaagg aatttccgcg
      901 agcagatata attgtaaata ttcagggcga tgaaccattt attagcggaa tgatgattga
      961 tcaggcaatt gagccgttac tatttgatga ggaagttaat gtttcaactc ttgcaaagtt
     1021 agttgaagat attgaggagt ttaaatcacc ctctgttgtt aaagttgttt ttgattataa
     1081 aaattttgct ttgtacttct cgcgatcacc aattcctttt gtacgagatg caaaagatga
     1141 aaaggaaatg attgagaagg caaatattta caaacacatt ggattgtatg tttatcgtaa
     1201 agaatattta ttgaaattta caaaacttga gccgactgat ttagaacaat gggaaaaact
     1261 tgaacagctt agaatgctgg aaaatggatt taaagttaag attgttgaaa ccgagtttga
     1321 tagtttttcc gttgatactc ctgaagattt agagaaggca agaaaaattt attcaaaact
     1381 aaaaaaaatt gagttagcag aatgatcact ccccaaaaga tttcacttgc acaaattcca
     1441 actccgttag aggaaataaa gtttgaaggc aaaagttttt taattaagcg agacgactta
     1501 acaggatgtg aattatcagg caacaaagta agaaaactcg agtatctgct tgctcatgca
     1561 aagcttaaaa aagcggatat tatttttact cctggcgggg atcaatcaaa tcatgccaga
     1621 gcaacagtta ttgccgccag aaaaatcgga ctaaaaacaa aattattttt atggggtaaa
     1681 gaaactccat atcccaatgg aaatcttttt cttgataaag catttggagc agacatccaa
     1741 tattttacaa aagccgagta tgaaaaagtt aatgaaataa tgttcgagca aagattagct
     1801 ttacttaaga agaaaatgaa tgcatatgtt tttcctgcag gtggatcaac tactcttggc
     1861 atttggggct atataaattt tatcaatgaa cttaaaaatc aggttgatct taaaaaaata
     1921 gattcaattg ttgcagcttg tggttccgga gggaccgcag caggaatgtt agtcggggca
     1981 gcattaaata atcttactct taaaatagta gctgttcatg tattgatgac aaagaaagag
     2041 atggaaaaac acattttaca acttgctgaa ggctgtgtgc ttgattataa attaaaatgt
     2101 aaaatcaatc cagaaaatct tattgttatg gacggatact ctaacgaagg ttataaaaaa
     2161 atatctgcat caaaagtaaa gttaataaaa aaatttgcgc aggagacagg aattctattt
     2221 gatccggctt acacgggaaa agctttcaca gcttattatg aaagatatct gaataccgga
     2281 agaggaaaga aaaatatttt tgttcacacg ggtggtttgt ttggcgtatt tggcagaacg
     2341 aaggaatatc tttcaaatta gaattgcatt ggtagttatc agtaaaataa tttaattttg
     2401 ttcaacaaat ataaagcgag cataatatgg attgtatatt ttgtgaaatt gcatcaaaat
     2461 cctctgaagc cgaaatcgtt tttgaaaatg ataaattcat agccttcctt gatataaatc
     2521 ctgttaatta cggacacaca cttgttgtcc ctaaaaaaca ttttgataac tttttaacta
     2581 tccccgccag c
//
LOCUS       DASWBY010000689         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_103553, whole genome shotgun sequence.
ACCESSION   DASWBY010000689 DASWBY010000000
VERSION     DASWBY010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103553"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..749)
                     /locus_tag="VF870_12600"
     CDS             complement(237..749)
                     /locus_tag="VF870_12600"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9253078.1"
                     /translation="MESKSKTLTDSELKKIIIKLGSDDGLERQKARESLVKGGRDSID
                     YLMELLSHPKHIYRWEAVKTLEEIGDPISIPILIQALEDDKGDVRWIAAKGLIKLGKE
                     SIVPILRTLEEKSDSVFVLEGAHHVFFDLRENKKLPKGFPIDKLLAALKNPEWVESVK
                     PLVYKILNET"
     gene            complement(770..3223)
                     /locus_tag="VF870_12605"
     CDS             complement(770..3223)
                     /locus_tag="VF870_12605"
                     /EC_number="7.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasma-membrane proton-efflux P-type ATPase"
                     /protein_id="HEX9253079.1"
                     /translation="MKKEILNIDEQKKLTVEEIFKKFESSDKGISASSAKERIELYGY
                     NEISEKKVNPFLKFLSYFWGPIPWMIEVAAILSALIHHWEDFWIIFALLLLNAVVGFW
                     QEYKADDAISLLKQKLALNAKVFRDGKWSEIPARELVPGDVVRARLGDIIPADLKLFS
                     GDYLSIDESALTGESLPVEKHKSDLAYSGSVIRQGEMNGLVLATGLNTFFGKTAKLVS
                     EARTISHFQKAVIKIGNYLIVLAAFMVAIIFLVSFYRHESFLETLQFALVLTIAAIPV
                     ALPAVLSVTMAVGASVLAKKKAIVSKLVAIEEMAGMDILCSDKTGTITKNELTLAEVK
                     AFEGFISDEVLLFASLSSREEDKDPIDTAIIDKTKSLKTLSDKLGSYKIKDFKPFDPV
                     IKRSEATVIDDENKTFKVTKGAPQAILSLIQGSDKDRTTDIINKQIDEFALKGFRTLG
                     TAKTNEQGNWVYVGLIPLFDPPRDDSAETIKTALEMGVQVKMITGDHTAIAKQIAQKV
                     NLKTNIQEASAFLDKPDNEAIKIIETADGFAQVFPEHKYRIVELLQDKKHIVGMTGDG
                     VNDSPALKKADVGIAVAGATDAAKSAADIVLTLPGLSVIIDAIKESRKIFQRMNSYAI
                     YRIAETIRVLLFITLAIIVFNFYPVTAIMIVLLALFNDAPIMAIAYDNVKYSKEPERW
                     NMRVVLSMATFLGLIGVVSSFGIFYIGQEVLNLSKESVQTFIFLKLAIAGHLTIFLTR
                     TRGYFWSIKPSATMFWAAVITKILATFVAVYGWFITPISWELALFVWGYAIAAFLITD
                     FIKVRIYKLVDHQDIKFHR"
BASE COUNT         1103 a          634 c          519 g         1195 t
ORIGIN      
        1 taacggatta gcaatttctg caataatgac gatgctggca ttattaattt ttggatacat
       61 aaaaggaaga tttaccggat ctaaaccaat taaaagcgca atgcaaactg tgataattgg
      121 tggtatggca gcaagtgttg catttctttt agcaaaattt atagggtgac aaataaaaaa
      181 gtttacccta aataaaaatc tttgaaatgt gtatctaaac ttaatctctg attgaattaa
      241 gtttcattta atattttata aaccaatggt ttaacacttt caacccattc aggatttttt
      301 aatgccgcta ataatttatc aataggaaaa cctttaggaa gctttttatt ttctctcaaa
      361 tcaaaaaaga catgatgagc tccttctaaa acaaatacag aatccgattt ttcttcaagt
      421 gttctaagga taggaacaat ggattctttt cctaatttaa taagtccttt tgcagcaatc
      481 cagcgtacat cgcctttatc atcttctaaa gcctgaataa gtataggtat agagattggg
      541 tctccgattt cttccaatgt ttttaccgct tcccaacgat aaatatgttt tggatgcgaa
      601 agtagttcca ttaaataatc tatagaatct cttccgcctt ttaccaatga ttcacgggct
      661 ttttgtctct ccaagccatc gtcacttcct aattttataa ttattttttt taattctgaa
      721 tctgttaatg ttttactttt agattccatt gtatctcctt aaaattattt tatctgtgaa
      781 atttaatgtc ttgatgatca accaatttat aaattctgac ttttataaag tcagtaatta
      841 aaaatgcagc tatagcatac ccccacacaa acaatgctaa ttcccaggaa attggagtta
      901 taaaccaacc ataaacagcg acaaatgttg caagaatttt tgttatcact gctgcccaaa
      961 acatagttgc acttggttta atagaccaga agtatccgcg ggttcttgtt aagaatattg
     1021 ttaggtgacc tgcaattgct agttttaaaa aaataaaagt ctgaaccgac tctttcgata
     1081 agtttagaac ctcttgcccg atataaaata ttccaaagga actaactact cctattaaac
     1141 ctaaaaatgt agccatactt aatacaactc tcatattcca tctttcgggt tctttagaat
     1201 acttaacgtt gtcgtaagca attgccataa taggtgcatc attaaacaat gcaagcagaa
     1261 ctatcattat agcagtaacc ggataaaaat taaatacgat tattgccaaa gttataaaca
     1321 agagtacacg aatagtttca gcaattctgt aaatagcata actattcatc ctttgaaaaa
     1381 ttttacgact ctcttttatc gcatctatta ttactgaaag acctggtaag gtaagaacaa
     1441 tatcagctgc tgattttgct gcatcagttg caccagctac agcaattccg acatcagctt
     1501 tttttaaagc tggtgaatca tttactccat ctcccgtcat tcctacaata tgttttttat
     1561 cttgcagtaa ttctacaatc ctgtatttat gttccggaaa aacttgtgca aatccatcgg
     1621 ctgtttctat tattttaatt gcttcattat ccggtttatc taaaaatgct gaggcttcct
     1681 gaatgtttgt tttaagatta actttttgag caatttgttt tgcaatagca gtatgatctc
     1741 cggtgatcat cttaacttgg acacccatct ccagagcggt ttttatagtt tccgcagaat
     1801 catcccttgg cggatcaaag agaggtatta aaccgacata tacccaatta ccttgctcat
     1861 tggtttttgc agttccaagt gttcggaaac cctttaaagc aaattcatca atttgtttat
     1921 taataatatc agttgttcta tctttgtctg aaccttgaat gagtgataag attgcctgag
     1981 gcgccccttt tgtaacttta aatgttttat tttcatcgtc aataaccgtt gcttcacttc
     2041 tctttattac cggatcaaat ggtttaaagt cttttatttt atatgatcct agtttatctg
     2101 aaagcgtttt aagtgatttt gttttatcaa ttatcgcagt atctattgga tctttatctt
     2161 cttctcttga tgatagtgat gcaaataata aaacctcatc ggaaataaaa ccttcgaatg
     2221 cttttacttc agcaagagta agttcatttt ttgttatagt tccggtttta tcagaacaaa
     2281 gaatatccat tccagccatt tcttcgattg ctacaagttt gctaacgata gctttctttt
     2341 ttgccagtac agatgcgcct acagccatcg ttacagataa aactgcgggt aatgcaacag
     2401 gtattgctgc gattgttaga actaatgcga attgaagtgt ctcaagaaaa ctttcatgcc
     2461 tatagaatga taccaaaaaa ataatagcaa ccataaaggc agcaagtaca attaagtaat
     2521 taccaatctt tattactgct ttttgaaaat ggctgatagt tctggcttct gaaacaagtt
     2581 ttgctgtttt gccgaagaaa gtattaagac cagttgctaa gaccaaaccg ttcatttcac
     2641 cctggcgaat gacagaaccg gagtaagcta aatcggattt atgtttttca accggtaaag
     2701 attcaccagt taatgcggat tcatcaatag aaagataatc accggaaaaa agttttaaat
     2761 cggctgggat aatatctcct aatcgggccc taacaacatc tcctggaaca agctctcgtg
     2821 cgggtatctc actccatttt ccatcacgaa atacttttgc attaagcgca agcttttgct
     2881 taagtaaact tattgcatca tccgctttat actcctgcca aaaacccaca acagcattta
     2941 gtaatagaag agcaaatatt atccaaaaat cttcccagtg atgtattaaa gcagaaagaa
     3001 ttgctgcaac ttcaatcatc catgggattg gtccccagaa gtaacttaaa aactttaaga
     3061 aaggatttac ctttttttca gaaatttcat tatagccgta tagttcaatt ctttcttttg
     3121 ctgatgatgc tgaaatcccc ttatcactgg attcaaactt tttaaatatc tcttcaaccg
     3181 taagtttttt ttgctcatcg atatttaaaa tttctttttt cattttccct gtccttccaa
     3241 ttttatccgg aagaaataat cttaaattca attcatagag aatgccattt aagagtttat
     3301 ttgaataata atgtaaaaaa atctgatcaa atcaaaaaat aagtgtgtga tcaattgtac
     3361 ttataagata taatgtaaat cacaattgaa tctgacttaa agtcatttta tgtttgataa
     3421 ctgtcttagc gatcattaaa gtaatgcaag t
//
LOCUS       DASWBY010000690         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_291815, whole genome shotgun sequence.
ACCESSION   DASWBY010000690 DASWBY010000000
VERSION     DASWBY010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291815"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF870_12610"
     CDS             complement(<1..187)
                     /locus_tag="VF870_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253080.1"
                     /translation="MKSSLLLAAITICSTVFIGWGNVGHRIINTKTILSVNPEMSFWG
                     SWSDSLAYHGSDADNRKN"
     gene            complement(409..>2909)
                     /gene="carB"
                     /locus_tag="VF870_12615"
     CDS             complement(409..>2909)
                     /gene="carB"
                     /locus_tag="VF870_12615"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HEX9253081.1"
                     /translation="NIDPMGIHTGDSVVIAPVQTLSAKENFKLRSIGIKLIRHLGIIG
                     ECNIQYALDPNSEDYRIIEVNARLSRSSALASKATGYPLAFIATKLALGYALNEVVNI
                     ITQETSANFEPALDYVALKFPRWDLQKFQQVSTQLGSEMKSVGEVMSLGRSFEEVLQK
                     AIRMLDVGMQGFIGNQMSFVNLDKELSEPTDKRIFAIAVALKQGYSVDKIHDLTKITK
                     WFLYKMKHIVDLENHIANRKLKDVDTELMRYLKQNGFSDYQIAELTNSKELEVREYRK
                     SLNVTPVVKQIDTMAAEFPAQTNYLYLTYHGVEDDILLGEKDQIVVLGSGAYRIGSSV
                     EFDWCCVNAVNQVNKSGYKSIMINCNPETVSTDYDICDKLYFEQLTFERVLDIYDKEN
                     PRGVIVSMGGQVPNNLAMKLHKAGLKIIGTSPEQIDNAESRHKFSKILDTIGVDQPEW
                     EEVTTLEDAKLFAEKVSYPVLIRPSYVLSGAAMSIVLTKDELEMYLKKATDLNTEHPV
                     VISKFITDAREIEVDAVATEGELFCYAIAEHVENAGVHSGDATIVLPPQRTYLETMRK
                     VKMITREIAQKLEITGPFNIQFIAKDNEVKVIECNLRASRSFPFVSKTLKINFIEIAT
                     RLMLGEKVPRIDKSSFDLDYVGVKASQFSFTRLKGSDPVTGVEMSSTGEVACLGDDFN
                     EAFLKSLLAIGQRVPTKGVLLSTGTPKNKAELIDELKILQKMGLKFYGTKGTSEFYKE
                     NGDLNVEVLYRPFDNQEPSILSYMANGEIDLVINIPKTTEKVELDSDYIIRRKAVDLN
                     IPLFTNIQIAKRFVKALKVYSPSTLPVKSWDEYE"
BASE COUNT          901 a          568 c          472 g          968 t
ORIGIN      
        1 tgttttttcg attatccgca tccgatccat gataggcaag cgaatcagac caacttcccc
       61 agaaactcat ttcaggattt acagaaagaa ttgtttttgt gtttataatt ctatgcccga
      121 catttcccca gccgataaac acagttgagc aaattgtaat tgccgcgagt agtaaacttg
      181 atttcatttt catactgcaa gttacaataa ttcagtttta atcttgcaaa ttaggaccaa
      241 aattatctgc gatctttgcg aaataacttt gtggcttttg cgtgaagagg ttttaaatca
      301 tttcacaatc caacgaggat ggttacgcca aaagatcaca aagcaaatct aaatacaaaa
      361 tactcaaagg atgaaaagat tattgactat ctaagtctta aacttaaact actcgtactc
      421 atcccagctc ttaacgggaa gagttgaagg tgaataaact tttaatgctt taacaaatct
      481 tttggcaatt tgtatgttag taaaaagcgg aatgttgaga tcgacagctt ttcttcttat
      541 gatgtagtca ctgtcaagtt caactttctc ggtcgttttg ggaatattta ttacaagatc
      601 aatttctcca ttagccatat aacttagtat gcttggctcc tggttatcaa aagggcgata
      661 aagaacttca acattaagat caccattttc tttgtaaaat tctgaagtgc ccttcgttcc
      721 gtaaaatttc aatcccatct tttgcagaat ttttaattca tcaattagtt cagctttatt
      781 ttttggtgtt ccggttgaaa gcaatacacc ttttgttgga actctttgtc cgattgcaag
      841 aagacttttt aaaaatgctt cattaaaatc atcccccaaa caagcaactt ctccagttga
      901 tgacatttca actccggtta ccggatcact tccttttaat cttgtaaatg aaaactgaga
      961 agctttaacc ccaacataat caagatcaaa agatgattta tctattcttg gaactttttc
     1021 accaagcatt aatcgggttg caatttctat aaaatttatt ttaagagttt tagaaacaaa
     1081 tgggaaactt cttgaagcac gaagattaca ctcaataact ttaacttcat tatcttttgc
     1141 gataaactga atgttaaatg gtcccgtaat ttcaagtttc tgtgcaatct cacgagtgat
     1201 cattttaact ttacgcattg tttctaagta agtgcgctgc ggcggcaaaa caatcgtagc
     1261 gtcaccagaa tgaactccgg cgttttcaac atgctcagca atagcatagc agaaaagctc
     1321 gccttcagtt gcaactgcat caacttcaat ttcgcgtgca tcagtaataa acttgcttat
     1381 aacaacagga tgctcagtgt taagatcggt agctttttta agatacatct caagttcatc
     1441 ttttgtaagc actatgctca tagctgcacc gctaagaacg taacttggac gaatcaaaac
     1501 aggatagctt actttttcag caaaaagttt tgcatcttct aaggttgtca cttcttccca
     1561 ttcaggttga tcaactccga ttgtatctag tattttagaa aacttatgtc gactttcagc
     1621 attatcaatt tgttcaggag aagttccgat aatttttaat cccgctttgt gaagtttcat
     1681 tgcaagattg tttggaacct gtccgcccat cgaaacaatt actccgcgtg gattttcttt
     1741 atcataaata tcaagcacgc gttcaaaagt taactgttca aaataaagtt tatcacaaat
     1801 gtcataatcg gtacttacag tttcagggtt acaatttatc ataattgatt tgtaaccgct
     1861 cttgttaacc tggttaacag cattaacaca acaccaatca aattcaactg aagaaccaat
     1921 acgatatgca ccgcttccaa gaacaacaat ctgatccttt tcgccgagca gaatatcatc
     1981 ctcaactccg tgataagtta agtacagata atttgtttgt gcgggaaatt cagcagccat
     2041 tgtatcaatc tgtttaacca cgggagttac atttaatgat ttacgatact cgcgtacttc
     2101 aagttctttt gaatttgtaa gttcagcaat ctgataatct gaaaatccat tttgtttaag
     2161 atatcgcatt aattctgtat caacatcttt taattttcta tttgcaatat gattttcaag
     2221 atcgacaata tgcttcatct tatacaaaaa ccattttgtg atttttgtta gatcatggat
     2281 cttatctaca gaatatcctt gtttaagtgc tacagcaatt gcaaaaattc tcttgtcggt
     2341 tggttcagat aattctttat caagatttac aaaactcatt tggttgccga taaacccctg
     2401 cattccaaca tcgagcatac gaattgcttt ttgcagaact tcctcaaaac tcctaccgag
     2461 agacattact tcgccgactg atttcatttc agaaccaagc tgagtgctta cctgctgaaa
     2521 tttctgtaaa tcccatcgcg gaaattttaa tgcaacataa tcaagtgcag gttcaaaatt
     2581 tgcagaggtt tcttgtgtga taatatttac aacctcattg agtgcgtaac caagtgcaag
     2641 ttttgtcgca ataaatgcaa gcggataacc ggtagctttt gatgcaagcg cagaactgcg
     2701 actaagtcga gcatttactt caataattct atagtcttca gagtttggat caagagcata
     2761 ttgaatattg cattcgccaa taattccaag atgacgaatt aattttattc caattgaacg
     2821 aagtttaaaa ttttcttttg cagaaagagt ttgcacagga gcaattacaa cgctgtcgcc
     2881 agtgtgtatt cccataggat ctatgtttt
//
LOCUS       DASWBY010000691         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_179258, whole genome shotgun sequence.
ACCESSION   DASWBY010000691 DASWBY010000000
VERSION     DASWBY010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179258"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VF870_12620"
     CDS             complement(<1..548)
                     /locus_tag="VF870_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253082.1"
                     /translation="MRQDPYAILEIKAEENVYNYSKTGLLIFNHPDSLNPAALLGSLG
                     DLIILEGDNLFIYTDSSSKNKFFFESDGRILNVNDRMNSISIPDNDSMIPWFEKMNER
                     DFSGLQFIHFSSKMPEGYLPYLTKLSEIKPDAGLFFEDDFNKMAALLKIFKPRYIVGA
                     ELHTSDYGLLSGLNNLEVLMVS"
     gene            complement(940..1950)
                     /locus_tag="VF870_12625"
     CDS             complement(940..1950)
                     /locus_tag="VF870_12625"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEX9253083.1"
                     /translation="MKYNRITLSERVEIYRHIQSGKSDRFIAEYLGRHHSTISREIKR
                     NIYPFHYIPIQGYGVITAQEKSEIRASSRRESKCKLSRNHRLREVVLEKLRLRWSPVQ
                     ISKYLSITYKEDPQMQISHESIYTYVYILGRGSLKKELYSYLRQKKLVRNYYKREEHQ
                     REFEDMISIEERPVSVAMRTIPGHWEGDLIIGKEQKSAIGSIVERTTRFLILVPLKSR
                     DAETVRKAFSRELKQLPKQVRLSLTYDQGSEMSQHKLFTKETKMKVYFAHPHSPWERG
                     TNENTNMLIRDFFPKGIDFNQVPRYKIKKVQTMLNERPRKILDWENPATVFNRILQQS
                     NK"
     gene            2276..>2999
                     /locus_tag="VF870_12630"
     CDS             2276..>2999
                     /locus_tag="VF870_12630"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9253084.1"
                     /translation="MNIVFNYELCTGCGLCSKVCGSKRIVIGDDRKPYKIVRLGCNDC
                     GHCVAVCPENAVRNTRVDMREFTDMVDPGISTEQFKHLVRNRRSIRNYKNEPLKKEHI
                     DKLLEVVKYIPTGSNRQLLKYKIITDEGLLLKIKTAMASKMKRVSKVSNSFPVRYFVT
                     GYRRRSLGRLTELWDNGTDTYLRGAPCLLIIYAEEKYFKITEWDAGIASNTIDLAAQT
                     LGLATLMNGYFVILSNRYKSIRK"
BASE COUNT          893 a          563 c          555 g          988 t
ORIGIN      
        1 atggaaacca tcaatacttc aaggttattc aggcctgaca gcaatccata gtcgctggta
       61 tgaagttcag cgcccacaat ataacgcggc ttgaatatct tcaaaagagc tgccattttg
      121 ttaaaatcat cttcgaaaaa taaacccgca tccggcttga tttcagatag tttagtgaga
      181 taagggagat aaccttccgg cattttagag ctgaaatgga taaactggag tcctgaaaaa
      241 tccctttcat tcattttttc aaaccacggg atcatgctat cattatccgg gattgaaatg
      301 ctattcatcc tgtcatttac attcagtatc cggccatcac tttcaaagaa aaatttgttc
      361 tttgaggaag aatcggtata aataaataga ttatcacctt caagaatgat caaatcaccc
      421 aaacttccta ataatgctgc aggatttaaa ctatccggat ggttgaatat cagcaatcct
      481 gttttggaat aattatacac attttcttca gctttaattt ccagtatggc ataaggatcc
      541 tgtctcactg tgcatgaggc gaataacagt atcattccag ttaataacgg gaaaaatgga
      601 tagtttctat aagaacagca tctcatgctg aaattattaa ggcttttcat ggtgtcgggt
      661 attaactaaa ttaataatca aaatgttaaa gcaagttatg tcaatttccc ggataaacct
      721 ccaaaaattt gtggaaaagt ctttcaatat cttaactgcg gtaattctta cttaccgatc
      781 ctttcgctcc gggagcagtt gaaagccggc gttcaaatcc ttaaatctgt taattccaaa
      841 ttttttatca acttcgattc aaagatttag tgcgacattt gttcaaaaat aacatattat
      901 taaacatatt actaaaagga tatttttctt gattatttgt catttattac tttgctgaag
      961 gatcctatta aatacagtag caggattttc ccagtctagt atttttctag gtcgttcatt
     1021 aagcatggtt tgaaccttct ttattttgta cctgggtact tgattaaaat ctatgccttt
     1081 cggaaaaaag tctcttataa gcatgtttgt attctcattc gttccccgct cccatggact
     1141 atgaggatgt gcaaaataaa ctttcatctt tgtctctttt gtaaataact tatgttgact
     1201 catctcactc ccctggtcat atgtcaatga gagtcgtact tgtttaggta actgttttaa
     1261 ttctctcgaa aaagccttac gtactgtctc tgcatcccta ctttttaagg gtacaagaat
     1321 caaaaaccgt gttgtacgtt ccacaatgct tccgattgca cttttctgtt ctttccctat
     1381 tatcaagtct ccttcccagt gacctgggat tgttcgcatt gccactgata cagggcgctc
     1441 ttctatactg atcatgtctt caaattcccg ttggtgttct tcccgtttgt aatagttcct
     1501 taccagcttt ttttgtcgta aatatgaata aagttccttt ttcagtgagc ctctacccag
     1561 aatataaacg taggtataga tactttcgtg acttatctgc atctgtggat cttctttata
     1621 ggttatggaa agatacttag aaatttgcac gggcgaccag cgaagtctca gtttctccaa
     1681 aacaacttcc cgaagtctgt gattgcgtga gagcttgcat tttgattctc tgcgtgatga
     1741 ggctctgatt tcactttttt cctgggccgt tataacccca tatccctgta tgggaatata
     1801 gtgaaacgga tatatgtttc gtttaatttc ccggctaatt gttgaatgat gacgtcccag
     1861 atattctgct ataaaacggt cactctttcc tgactgtatg tgcctgtaga tctctactcg
     1921 ttcacttaag gttatccgat tgtatttcat gtgcgcaaac taaatgaata ttatttagtg
     1981 tcgcactaaa tccttgaatc tacccattat aattatcaac gtattaaaag ccgtttataa
     2041 aaacggcttt taatacgttg gaatattata ttttcaacta atgatttact tcggttgaaa
     2101 gcgaagcaat attcagggag gtttcttatt cctgattaat aacaggattt ttagttaaat
     2161 attcttcatc ctgttacctg ccagcctcag caaaatattt gataccgtgc ttacatttgt
     2221 ttattaaaaa gaatcatctt tgcacaattg ttaaaataaa attatcagga tacaaatgaa
     2281 tattgtgttc aattatgaat tatgcaccgg atgcggatta tgcagcaagg tatgtggttc
     2341 aaaaagaatt gtgatcggtg atgacaggaa gccgtataaa attgtcagat taggctgtaa
     2401 tgattgtggc cattgtgtcg cagtctgccc tgaaaatgca gtccggaata caagagtgga
     2461 tatgagggaa tttactgata tggttgatcc cggaatttca acagaacagt ttaagcatct
     2521 tgtgagaaac aggcgaagca tcaggaatta taaaaatgaa cctttgaaaa aagagcacat
     2581 tgacaaattg cttgaggttg ttaaatatat tcctaccgga tcgaacaggc agttgcttaa
     2641 atataaaata attactgatg aaggcttatt gttgaaaata aaaacggcaa tggcatcaaa
     2701 aatgaagagg gtatcaaaag tcagtaattc ttttccggtc aggtattttg tcacaggcta
     2761 cagacggaga agccttggca ggctgacgga attatgggat aatggtacag atacatacct
     2821 gaggggggca ccctgccttt tgatcatata cgccgaagaa aagtatttta agatcactga
     2881 atgggatgcg ggaatagcca gtaatactat tgaccttgcc gcccagaccc tcggactggc
     2941 aaccctgatg aacggttatt ttgtcattct ttcgaatcgc tataaatcta tcaggaagc
//
LOCUS       DASWBY010000692         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_592518, whole genome shotgun sequence.
ACCESSION   DASWBY010000692 DASWBY010000000
VERSION     DASWBY010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592518"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF870_12635"
     CDS             complement(<1..287)
                     /locus_tag="VF870_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253085.1"
                     /translation="MRVVKIFLAFMLFYSVAVFGQFGKNKMHIKDYDWYYIQTKHFDI
                     YFSQDGETLTEFTAKVAEDAVASIQNTFNYRLNNRVTIIIYNSQNEFQETNV"
     gene            complement(295..2394)
                     /gene="rnr"
                     /locus_tag="VF870_12640"
     CDS             complement(295..2394)
                     /gene="rnr"
                     /locus_tag="VF870_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX9253086.1"
                     /translation="MKKELKAFFNRNQGRAYKSKDIANKLGFNTEYEYASLKAALHKL
                     EEESFLIRTGKRYQLNKLPQSNRLKGRLEINRNGFGFVNINDKDRGDVFIAARNIGTA
                     FDGDIVEVELFAKQKGKNIEGQIVNVVERKRKEYVGIIKKSKSFFFISADDPKLHRDI
                     YINESKLNGAKTGDKVVVGKLIWDTSMLNPEGEVVEVLGKAGTHDTDIISIAREFDLK
                     YKFPASVLSEAEGISNIIPKEEIKKRLDYRSKNVFTIDPEDAKDFDDALSIEKLENGN
                     LSIGIHIADVSHYVKKGSALDIEASERGNSVYFVGRVIPMLPEKLSNQICSLVPFEDR
                     LTYSVIVEMTPRGKIIDYIIEKTVINSKRRFTYEEAQKVIEDGTGDLAEDILLLNKIA
                     DQFRKKRMREGSFNFISPEIKFNLDENGVPVSIMIKQTKQSNMLIEEFMLLANKLVAQ
                     QIAIPKSGPVKPFVYRIHDLPDQEKILEFSRFVKTLGYTFDPNATSKSIGFQNLLNQV
                     KGKEEEALINELAIRSMAKAIYSPDNIGHYGLGFKYYSHFTSPIRRYSDLIVHRLLFN
                     YKNLKSGEIYSLAQLEEICENISASERNAVEAERFSVKQKQIEYLANHLGNEFSAIIS
                     GITNFGIFVKITDILAEGLIRLRDLDDDYYMHDEKKYAIIGKRTKKQYRLGDKIHVKL
                     IRIDEERSELDFIILES"
     gene            complement(2394..>3001)
                     /locus_tag="VF870_12645"
     CDS             complement(2394..>3001)
                     /locus_tag="VF870_12645"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX9253087.1"
                     /translation="ITSTYKDYYYPPNSSGGQTEQEVAVKIKLKGPLSELNKNFIKDE
                     NNIGVYMGRQAIEEDKKDPTKNATDAMYFLIIGKFPGDDNSQNDKNLVASTTTALAGS
                     LIGEVLNQYFDNYVTGFQLRQTGTETKFNLIGKVWKIKYEIGGSTEVLQDLSRANVKM
                     EYPITERLQLRLERKESENQTTSSVNNPLFFEGGFKYNFEF"
BASE COUNT          907 a          551 c          414 g         1129 t
ORIGIN      
        1 acatttgttt cctgaaattc attttgagaa ttgtaaatta ttattgttac tctgttattt
       61 aatctgtagt taaaagtatt ttgaattgaa gcaacagcat cctcagcaac tttagcagta
      121 aattctgtta aagtttctcc atcctgagaa aagtatatat caaagtgctt ggtttggata
      181 tagtaccaat cataatcctt gatgtgcatt ttattttttc cgaattgccc gaataccgca
      241 acggaataaa aaagcataaa cgctaaaaaa atttttacta cacgcattta tttcctatga
      301 ttccagtatt ataaaatcta attccgatct ttcttcatcg atacggatta gttttacatg
      361 aattttatca cctaaacgat attgtttttt agtcctctta ccaattattg catatttttt
      421 ttcatcatgc atataataat catcatctaa atctcttaac cttatcaagc cttcagcaag
      481 tatatctgta attttaacaa aaattccaaa atttgtaatg ccagaaatta ttgccgaaaa
      541 ttcatttccg agatgatttg caaggtattc aatctgtttt tgtttaacac taaaccgctc
      601 agcttcaaca gcatttcttt cagatgcaga tatattttcg caaatttctt ccaattgtgc
      661 aagagaataa atttccccgc tttttaagtt tttgtaatta aataataacc tatggactat
      721 caaatcacta tatcttctaa taggagaagt gaaatgtgaa taatatttaa aacctaagcc
      781 ataatgtccg atattatccg gagaataaat tgctttagcc atagacctaa ttgcaagttc
      841 atttattaag gcttcttcct cttttccttt aacctgatta agcagattct gaaatccaat
      901 tgatttggaa gttgcattag gatcaaaagt gtaaccaagt gtttttacaa agcgagaaaa
      961 ttctaaaatc ttttcctgat caggtaaatc atgaattcga taaacgaatg gttttacagg
     1021 accagacttt ggaattgcaa tttgttgtgc aacaagttta tttgccaaca acataaactc
     1081 ctctataagc atattgcttt gttttgtctg ctttatcatt atagaaaccg gaacaccatt
     1141 ttcatcaaga ttaaatttta tttccggtga tataaaatta aaactgcctt cacgcattct
     1201 cttttttcta aattgatcag caattttatt taacaataat atatcttcag ccagatcccc
     1261 cgttccatct tcaattactt tttgggcttc ttcgtatgta aatcttcttt tactgtttat
     1321 tacagttttt tcgataatgt aatcgatgat ttttcctctt ggtgtcattt cgacaataac
     1381 agaataagta agcctgtctt caaaaggtac gagtgagcaa atctgatttg atagtttttc
     1441 cggcaacata gggattaccc tgcctacaaa ataaacgctg ttgcctcttt cactggcttc
     1501 aatatcaaga gccgaacctt tttttacata gtgagaaaca tccgcaatat gaattcctat
     1561 agataagtta ccattctcaa gtttttcaat agaaagtgcg tcatcaaaat cttttgcatc
     1621 ttcaggatcg attgtaaata cgtttttact tctataatcc agacgctttt taatttcttc
     1681 tttgggaata atgttactaa ttccctcggc ttcagatagt accgatgcag ggaatttata
     1741 ttttagatca aactcgcggg caatagaaat aatatctgta tcgtgagtgc cggctttacc
     1801 aagtacttca acaacttcac cctccggatt aagcattgat gtatcccaaa tcaacttgcc
     1861 aacaacaact ttatctccgg ttttagcacc atttaattta gattcattta tgtaaatgtc
     1921 cctatggagt tttggatcat cagctgaaat aaaaaagaat gattttgatt tttttattat
     1981 tccaacatat tctttacgtt ttctttcaac aacatttaca atctgtccct caatattctt
     2041 tcccttttgt ttagcgaata attcgacctc aacaatgtcg ccatcaaatg cagttccaat
     2101 atttcgtgcg gctataaata catcgccgcg atctttatcg ttaatattta caaatccaaa
     2161 accattgcgg tttatttcaa gtctgccttt caatcggttt gattgaggaa gtttatttaa
     2221 ctggtatctt tttcctgttc ggataagaaa agattcttct tctaatttat gaagggctgc
     2281 ttttagcgaa gcatattcat actctgtatt aaacccaagt ttatttgcaa tgtctttgga
     2341 tttatatgct ctgccttgat ttctgttaaa gaaagctttt aattcttttt tcattaaaac
     2401 tcaaaattat atttaaaacc accttcaaag aaaagtggat tgtttactga tgatgttgtc
     2461 tgattttcag attcttttcg ttctaagcgc aattgaagtc tttctgtaat aggatattcc
     2521 atcttcacat ttgcacggct taaatcctgc agcacttctg ttgatccgcc gatttcatat
     2581 ttaattttcc aaactttgcc tataagatta aacttagttt cagtacctgt ttgtcttaac
     2641 tgaaatcctg ttacataatt atcaaaatat tggtttagta cttcgcctat taaagatccg
     2701 gcaagtgctg ttgttgtgga tgcaactaaa tttttgtcat tctgactatt atcatcgcct
     2761 ggaaacttac caattattaa aaaatacatt gcatctgttg cattttttgt aggatctttt
     2821 ttatcctctt caattgcctg acgccccata tatacaccaa tattgttttc atctttaata
     2881 aaatttttat tcaattcact caaaggtcct tttaatttta ttttaactgc aacctcctgt
     2941 tcagtttgac caccagatga attgggagga taataataat ctttatatgt agatgtgata
     3001 t
//
LOCUS       DASWBY010000693         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_481424, whole genome shotgun sequence.
ACCESSION   DASWBY010000693 DASWBY010000000
VERSION     DASWBY010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481424"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..644)
                     /locus_tag="VF870_12650"
     CDS             complement(96..644)
                     /locus_tag="VF870_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX9253088.1"
                     /translation="MKSIKYFISLTFITFVTAVLLVGCSTISVNQDYDPGYDFSKLKT
                     FGFLPIPKEAGIDQINANRFGDAIKNELTAKGYTVSDKADFGVALLFGKQTKTNITSY
                     GYGYGPYWGRYGGTSNVDVSQYDEGTLIIDVIDMAKKELVWRGTGTGAIDPNATVEER
                     TANINSAVAQILAQFPPTPVQK"
     gene            complement(730..1701)
                     /locus_tag="VF870_12655"
     CDS             complement(730..1701)
                     /locus_tag="VF870_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX9253089.1"
                     /translation="MNPNWCAIIVLFIISGFCFQQSIAQMPARFYWKTLSGANGIPLI
                     VESISGNTNPFDPSHNVNPGAYVSGTMAMVGYAKFLPLFDRSAMISVIFPMGRITSEV
                     ISTTLTSKETAKGFGDPMLEFNINVIGPKAQNSLPDLMRYEPGFSLDLMMDIGIPIGE
                     YNDTLALNLGGNRWFGRLAAPIIWQIGPWVPGRRTTLEFLPAVWLFGDNTDFLGQTMK
                     TDPILQLDAHLSRDITHNFWASLDGAWFNGGKATIGTLEGTKLNNIALGITLGYQINE
                     NINLTFGYKSTINDDAADALKMDGFMVTLVYGWHPLIEGMKRLKESE"
     gene            complement(1779..>2400)
                     /locus_tag="VF870_12660"
     CDS             complement(1779..>2400)
                     /locus_tag="VF870_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253090.1"
                     /translation="MTPFSQYKAWMGEGGIRNALIVSGPVVKRPAGSINNGQMHVVDI
                     MPTLLEIANVKYPEKSEGGRELHPLIGKSWVNVLTGKSESPRTEKDYMAWEIFGNRAL
                     IQGDWKIRWEFKPIGKGDWELFNLANDSGERKDLAKENPEKLNALLKLWDKYVKENNV
                     IIPNRSMFEALEDAFPPRVPVEGGYPPLNYKRQFVPPQDMLAEPKK"
BASE COUNT          695 a          515 c          404 g          786 t
ORIGIN      
        1 ttacatattc ctttataatt gtttaaggta attaattaag tgatttattt taaaacttat
       61 atttatcata atattagttt tcatagctcc attatttatt tctgaaccgg tgtcggtgga
      121 aactgcgcaa gtatctgcgc aacagcacta ttaatatttg cggttctctc ttcaactgta
      181 gcgtttggat ctattgcacc tgttccggta ccacgccata ctaactcttt cttagccata
      241 tcgatgacat caatgattag agtaccttca tcatactgtg atacatctac atttgaagta
      301 ccaccatatc tgccccaata aggtccgtat ccataaccat aagaggtaat gttagttttt
      361 gtctgctttc caaaaagtag agccacacca aaatctgctt tatcagacac ggtatatcct
      421 tttgcagtca attcattctt gattgcatcc ccaaaacggt tagcatttat ttggtcaatt
      481 cctgcttctt ttggaatagg aagaaatcca aaggttttta gctttgaaaa atcataacct
      541 ggatcataat cctgattaac agatattgtt gaacatccaa ctaataatac agctgtaaca
      601 aacgtaataa aagttagact aataaaatat tttattgatt tcatatgagc acctttcttt
      661 taatttatta attagttaat aataagtgat ttatatacac tcaacttcag cgatttaata
      721 atttattact tactcacttt cctttagtcg tttcatacct tcaatcagcg gatgccatcc
      781 gtacacgagt gttaccataa agccatccat tttaagtgcg tccgcagcat catcatttat
      841 tgttgacttg taaccaaaag taagattaat gttttcatta atctgatatc cgagtgtaat
      901 accgagagcg atattgttaa gttttgtacc ttctaaggta cctatggttg ctttgccgcc
      961 gttaaaccat gcaccatcca gcgatgccca gaaattatgt gtaatatctc ttgagagatg
     1021 tgcatctaat tgtaatatag gatcggtctt catcgtttgc cctaggaaat cagtgttatc
     1081 tccaaacagc cagaccgccg gcaagaattc cagagttgta cgcctgcctg gcacccatgg
     1141 accaatttgc cagataattg gggcagctaa tcttccgaac cagcgattcc ctccgagatt
     1201 tagtgccaat gtatcgttgt attcaccaat cgggatacct atgtccatca tcaggtcaag
     1261 tgaaaatccg ggttcataac gcataagatc gggcagacta ttttgcgcct taggaccaat
     1321 gacattaata ttgaattcga gcataggatc gccaaaacct tttgcagttt ctttagaagt
     1381 caaggtagtt gaaataacct cactggtaat gcgacccata ggaaagatga ctgaaatcat
     1441 tgctgaccga tcaaacaatg gtaaaaattt agcgtaccct accattgcca tcgttccact
     1501 tacataggct cccggattta cattatgtga agggtcaaac ggatttgtat tgccgcttat
     1561 tgattcaaca attaagggta taccattggc accggataaa gtcttccaat aaaaacgagc
     1621 tggcatttgt gcaatgcttt gctgaaaaca gaacccgcta attatgaaca acacaataat
     1681 ggcgcaccaa ttcggattca tttttgattt agataatata tattctttca ttatttcacc
     1741 ttctatattt attaagtttt attttttcca ccacgattct atttctttgg ttcagccagc
     1801 atatcctgcg gtggtacaaa ctgtcgctta taattaagcg gcggatagcc tccctcaacc
     1861 ggaacacgtg gaggaaaagc atcttcaaga gcttcaaaca tagaacggtt cggaattatt
     1921 acattattct ctttaacata cttatcccat agtttaagaa gtgcatttaa cttttcagga
     1981 ttttcttttg caaggtcttt tcgttcaccg gaatcatttg ccagattaaa tagttcccaa
     2041 tctcctttcc cgataggttt aaattcccac cgaattttcc aatctccctg tatcaaagca
     2101 cggttaccaa atatttccca tgccatatag tccttttcag ttcgtggtga ttctgactta
     2161 ccagttaata catttaccca cgatttacct atgagtggat gcagttcacg gccgccttca
     2221 gatttctcgg gatatttaac atttgcaatt tcgagtaaag ttggcataat atctacaacg
     2281 tgcatctgtc cgttgttgat acttccggcc ggtcgtttga cgacaggacc actaacgatt
     2341 agagcgttac gtataccacc ttcacccatc caagccttgt actgactaaa tggcgtcatt
//
LOCUS       DASWBY010000694         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_179482, whole genome shotgun sequence.
ACCESSION   DASWBY010000694 DASWBY010000000
VERSION     DASWBY010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179482"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF870_12665"
     CDS             <1..360
                     /locus_tag="VF870_12665"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX9253091.1"
                     /translation="GKLLEGTLLTDAIKIIDDSVLTPPLFYLTNCIHPDVLHKSFFNL
                     KDENDILKKRLFGIQANASSKSPEELDTLEKLDADSPENWAKGMVDLNKKYNLKILGG
                     CCGTDARFISSIVDLLK"
     gene            complement(430..1893)
                     /locus_tag="VF870_12670"
     CDS             complement(430..1893)
                     /locus_tag="VF870_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HEX9253092.1"
                     /translation="MKALFIFFLLSIAILPQNNPKFDDYFLDETLRIDYFHIGDAKTE
                     MITVDKLFRYGIWAGSLNHPVDNLNNGKYYVMIYDAATNKLIYSKGFDALFGEYASSD
                     DGVNGIVKSFHESAIIPFPKNKIIFALEKRNDKNLLDEFFRTEIDPASIFIIKDKVSD
                     AAVEILKPVYNGDPHTKVDIAILAEGYTKSEREKFEKDLNKFVEFFFEQEPYKSQKNN
                     FNIYGVFKPSEESGTDLPGADIFVNTVLNTTFWTLGSERYLMTEDNLSMRNLAAHVPY
                     DAIYIQVNHPRYGGGGIYNQYCTYTTDNQFAKYLFTHEFGHSFTGLADEYYTSDVAYN
                     DFFKPTVEPVEPNITALLDPKNIKWKKYLSDGIEIPTPWEKEEFDRFSYEWLKERNRL
                     NSFISELKRNRAPESEIKAAEEEYAMKDKKQSEKVDKYLMSSKYWNKVGAFEGGGYQP
                     KGIYRPMIDCIMFSKGDKPFCKVCEESIKEVIDSYTK"
     gene            2114..>2593
                     /locus_tag="VF870_12675"
     CDS             2114..>2593
                     /locus_tag="VF870_12675"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEX9253093.1"
                     /translation="MNFYTRSLNFRFSLLLSLIISIYCVAQQDESWKLYDDSQVARVD
                     ININPAAIEWLYNNVQSDSEFVASFHFQNAYINEVVDSIGFRLRGNTSREADKKSFKV
                     SFNTFVQGREFYGVDKLNLNGEHNDPSIIRSKLCFDHYQTIGMKASRANHVEVYINGQ
                     "
BASE COUNT          834 a          440 c          416 g          903 t
ORIGIN      
        1 gggaaactac ttgaaggcac tctgctaact gatgcgatca aaataattga cgattcggtt
       61 ttaactccgc cattatttta tttaacaaat tgtattcacc cggatgtact tcataaatca
      121 ttttttaatc ttaaagatga aaatgatatt ttaaagaaaa gattatttgg tattcaggca
      181 aatgcttcta gtaaaagtcc ggaagaactt gacactcttg aaaaactcga tgctgattct
      241 ccagaaaact gggctaaggg aatggttgac ctgaataaaa aatataatct taaaattctt
      301 ggtggctgct gcggaacgga tgcaagattt atttcttcta ttgttgattt actaaaataa
      361 acttagcgac ttagcgtctt tgcaagaaac agaaaaaaaa tctcgcagag acgccaagac
      421 gcagagtaac tacttggtat aactatcaat aacttcctta atcgattctt cacaaacttt
      481 acagaatggt ttatctcctt ttgaaaacat aatgcaatca atcatcggtc tgtaaattcc
      541 ttttggctga tatccaccgc cctcaaacgc accaacttta ttccaatact tgctgctcat
      601 taaatattta tcaacttttt ctgactgctt tttatctttc attgcatatt cttcttctgc
      661 ggcttttatc tcactttcag gagcacggtt tctttttagc tcggatataa aactgtttag
      721 tctatttctt tcctttagcc attcataact gaatctgtca aattcttctt tttcccaggg
      781 agttggtatt tcaattccat cgcttaaata ttttttccac ttgatgtttt ttggatcaag
      841 cagtgctgtt atgtttggct cgactggttc aactgttggt ttaaagaaat cattatacgc
      901 aacatccgaa gtgtaatatt cgtcagctaa tccggtaaat gaatgaccga actcgtgtgt
      961 gaataaatat tttgcaaact ggttatcggt agtgtatgtg cagtattgat tataaattcc
     1021 gccgccgccg taacgaggat gatttacctg aatataaatt gcatcataag gaacatgagc
     1081 ggcaaggttg cgcattgata aattatcttc agtcattaaa tatcgctctg aaccgagagt
     1141 ccagaaagtt gtgttaagaa ctgtgttaac aaatatatct gctcccggta aatctgttcc
     1201 tgattcttcc gaaggtttga acacaccata aatattaaaa ttattttttt gtgatttata
     1261 aggttcctgt tcaaagaaaa attcaacaaa cttatttaaa tctttttcaa atttttctct
     1321 ttcggatttt gtataacctt cagcaagaat agcgatatca acttttgtat gaggatcacc
     1381 gttataaacc ggttttaaaa tctcaactgc tgcatccgat actttatctt taattataaa
     1441 gatacttgcc ggatcaattt cagttctgaa aaattcatca agcagatttt tatcatttct
     1501 tttttccaaa gcaaaaataa ttttgttttt agggaaagga attattgctg attcgtggaa
     1561 actctttaca attccattta cgccatcatc actcgatgca tattcaccaa aaagtgcatc
     1621 gaatccttta gagtatatta atttattagt ggcagcatca taaatcataa cataatattt
     1681 cccgttgtta aggttatcaa caggatgatt aagacttcca gcccaaatac catatcggaa
     1741 cagcttatca accgttatca tttcagtttt tgcatcaccg atgtgaaagt aatctatacg
     1801 taatgtttca tccagaaaat aatcatcaaa ctttggatta ttttgaggta gaattgctat
     1861 tgatagtaaa aagaaaatga ataaggcttt cattgcttcc tcgcggatat tttatgcaaa
     1921 ttattttttt tgtgatagta atttaataat cttttggtga actttagata gttaaaacat
     1981 ttgagatctt cgttcgggtc attccgaact tgtttcggaa tattaaatac ttatcctgaa
     2041 atgaatccag gatgacaatg acaaataaat attttaaagt ttgaagtttt tatactaaac
     2101 aattggatac aaaatgaatt tttatacaag atctttgaat ttcagattct cactcttact
     2161 ctcactcatt atctcaatct attgcgttgc tcaacaggat gaaagctgga aactttatga
     2221 tgactctcaa gttgcaagag tagatatcaa tattaatcca gcagcaattg aatggttata
     2281 taataatgtt cagagtgatt cagaatttgt agcatcattt cattttcaaa atgcttacat
     2341 aaatgaagtg gttgattcta ttggatttag attacgaggc aacacttcca gagaagcgga
     2401 taaaaaatct tttaaagttt cctttaatac ctttgtgcaa ggaagagaat tttacggagt
     2461 tgataaatta aatttaaatg gcgaacataa tgatccatca atcatacgca gcaaattatg
     2521 ctttgatcat tatcaaacaa ttggaatgaa agcaagccgg gcaaatcatg ttgaagttta
     2581 tattaatgga cag
//
LOCUS       DASWBY010000695         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_141954, whole genome shotgun sequence.
ACCESSION   DASWBY010000695 DASWBY010000000
VERSION     DASWBY010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141954"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VF870_12680"
     CDS             complement(<1..221)
                     /locus_tag="VF870_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253094.1"
                     /translation="MNSNKTIESIKLNDLFKNLDFTSLDIPFDAKNFLEFKEGDLIYS
                     SGQPSDFVYLLINGEVKIKLNSIKRLFFKS"
     gene            384..944
                     /locus_tag="VF870_12685"
     CDS             384..944
                     /locus_tag="VF870_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9253095.1"
                     /translation="MEESKLVELAKSGDRQALAQLVKNNEQTVYNFSFKICRDRDKAE
                     HTMQETFLSMIKSLHQFDGNSKLSTWLYRIVSNHCLMLARKDKSKTFVSIDNDDELYE
                     DKYTADWSNIPNQNIENVELKKILDESIDKLSPEYRIVFLLRDVEGLSTEETAELTDL
                     SIPAVKSRLHRARAFLRKELNEAFSR"
     gene            941..1183
                     /locus_tag="VF870_12690"
     CDS             941..1183
                     /locus_tag="VF870_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9253096.1"
                     /translation="MSEQKPKCKDVMQHICESLGEELNSDKCVAIKNHLDNCSDCKSY
                     FKTVELTIDYYKKYNVEIPNEAHSRLMNFLDLSDCE"
     gene            1224..1466
                     /locus_tag="VF870_12695"
     CDS             1224..1466
                     /locus_tag="VF870_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX9253097.1"
                     /translation="MPIFEYKCKQCNSKFEVLHKSSLNQEEVSCPSCNSKENQKLLST
                     FSAAGFSSNGPSCESGNCGTAPSYGGGCSSGMCGLN"
     gene            1478..1849
                     /locus_tag="VF870_12700"
     CDS             1478..1849
                     /locus_tag="VF870_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9253098.1"
                     /translation="MNRFRNVIILAAVLLTSISYAQQDQTNSITMEQFKEKLKTDKEL
                     IVLDVRTPEEVAAGKIENSINIPVQVLEERINELEKYKDKEIFVLCRTQNRSSAAQSI
                     LKKNGYNAKYVIGGMSVYNQK"
     gene            2151..>4709
                     /locus_tag="VF870_12705"
     CDS             2151..>4709
                     /locus_tag="VF870_12705"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9253099.1"
                     /translation="MRFIIQSLSVFFLTIIFLGTAYSQSGKISGKVVDGNTGEALPFV
                     NIMVEGTTQGAASDIDGFYSIIGLRPGNYDVKASAIGYNSQTVTGVRVSIDLTTEVNF
                     QLKETSIELKEEVVVIATRPLVTKDLTSSTAIVSGDDISVLPVTEFQEVLQLKAGIVG
                     GNVRGGRSGEVVYAIDGVPVTDVYDGSTVVDVATSSIQELQFVSGAFNAEYGKALSGY
                     VNIATKDGDNKFQGSATSYIGDYVSNRTDIFRAIDQVNPTSIRDFEGSISGPIIPNQL
                     FFYVNARYIYFGGWLNGQNNYNPWNITINQGATVPIENRYILSANPDSGLGNGDIVSM
                     NWNEKLYGQGKLTYRPFSGVKLNFNYIYDNVEYRDFDQSFSYNPAGDYKKFRVGNTNI
                     LGLTHTLSPTTFYQANFSYFFKQFKQYVYEDVNDPRWTNDRLLSQEPTEVPSFRTGGT
                     QSQQFKRNTSTYGFKLDFTSQVDKINMVKFGIEANQHILGFDLINLQQPLSVPDPAVT
                     LNPFVTRYVPDINDPDQNLAIDIYQRKPIELSAYLQDKIELNEIIINVGFRLDYFDPD
                     GLVLNDITDPDIYRPRKDVNISKTLEERRASNAFGDPWYRKADTEMKFSPRLGIAFPI
                     TDRGVIHFSYGHFFQIPNFELLYQNPEFKFGAGTGNLGIAGNSNLKAQQTISGEVGVS
                     QAFTDDITVDITAYFRDIRNLAGTRADVIRLPGGSSSYSQYVNSDFGFVQGIILTLTK
                     RLSNNWSATIDYTFQSAKGNASDPAATRNQIAGGEQPEIKLIKLNWDQTNTVNVTFSY
                     TSDDNWGFSLIGQYGSGFPYTPTLSQNVSTLLINSELKPSFKNVDLRAYKDFIIS"
BASE COUNT         1690 a          749 c          838 g         1432 t
ORIGIN      
        1 gatttaaaaa ataatctctt aattgaattt aattttatct taacctcgcc gttgataagc
       61 agataaacaa aatcagaagg ctgacctgaa gagtaaatta aatctccttc tttaaattct
      121 aaaaaattct ttgcatcaaa aggaatatcc aatgaggtaa agtctaagtt tttaaacaag
      181 tcgtttaatt ttatggactc aattgtttta ttagaattca tagcaaaacc tgaaataatt
      241 aataattgaa aatatcttta caaatattgt gcaagaagaa aattggcaaa aaaccgctaa
      301 aagctaaaga tttacctatc taaatattat ttggtatttt tgtccactta aagaatccac
      361 attttacata gagcatctta cagatggaag aatcaaaatt agtagaactt gcaaaatccg
      421 gtgacagaca agcacttgct caattagtga aaaacaatga gcagacagtc tataattttt
      481 catttaagat ttgcagggat agagacaagg ccgagcatac gatgcaggaa acttttttaa
      541 gcatgataaa aagtctgcat cagtttgatg gaaactcaaa attatccacc tggctataca
      601 ggatagtttc taatcattgt ttaatgcttg ccagaaaaga taaatcaaaa acttttgttt
      661 cgattgacaa tgatgatgaa ctttacgaag ataaatacac tgctgattgg tccaacatac
      721 caaaccagaa tattgaaaat gttgagttaa aaaaaatatt ggatgaatca attgataaac
      781 ttagtccgga atacagaata gtatttttac ttcgtgatgt agaaggttta tcaacggaag
      841 aaacagcgga acttacagat ttatctattc cggcagtaaa atcccgattg cacagagcga
      901 gagcattttt aagaaaagaa ttaaatgagg catttagccg atgagtgaac aaaaaccaaa
      961 atgtaaagat gttatgcagc atatctgcga aagtttaggt gaagaactta attctgataa
     1021 atgtgttgct atcaaaaacc atcttgataa ttgctctgac tgcaagagtt attttaaaac
     1081 agttgaactt acgattgact actataaaaa atacaatgta gaaattccga atgaggcgca
     1141 ctctagactt atgaattttt tagacttaag cgattgcgaa taaattttat aatcatatta
     1201 gaataattta aaaggataat tttatgccga tatttgaata taaatgcaaa caatgcaact
     1261 caaaatttga agttcttcac aaatcatcat taaatcagga agaagtaagc tgcccaagct
     1321 gcaattcaaa agaaaatcaa aaattattat caacatttag tgcagcagga ttctcttcta
     1381 acggtccatc ttgtgaatca ggtaactgcg gaactgcacc atcttatggc ggcggatgtt
     1441 cttccggtat gtgtggatta aattaaaagg aaatagaatg aatagattta gaaacgtaat
     1501 aattttagct gcagtattat taacctcaat aagctacgca caacaggatc aaactaattc
     1561 cattacaatg gaacaattca aagagaaatt aaagactgat aaagaattaa ttgttctgga
     1621 tgtcagaact ccagaagaag ttgctgccgg taaaattgaa aattcaataa acattccggt
     1681 tcaggtttta gaagaaagaa taaacgaatt agaaaaatat aaagacaaag aaatatttgt
     1741 tctttgcaga acgcaaaaca gatcctctgc tgctcaaagc atacttaaaa aaaatggcta
     1801 taatgcaaaa tatgttattg gaggaatgtc ggtttataat caaaaataaa tagtttctca
     1861 actaagaaat ctgtttaata ctttactttt atgcaaacta ttcttcggaa ttcacctaac
     1921 agacttacct ttataattgg ctgctctcaa taaaaagagg attagcacaa aaaccaaaat
     1981 accttaataa aattattact ttgacattac ataagttgga atatatattt ggctacggaa
     2041 atttataaat aagcttatta aacttttatt aggacaaagt atagaattat ttctactcaa
     2101 ttcttataca attcaaatat gatctacaat tttacctcta ggaggaatgt atgaggttta
     2161 taatacagtc tttatctgta ttctttctga caattatttt tcttggaact gcttatagtc
     2221 aaagcggtaa aatctccgga aaagtagttg atggaaatac aggagaagcc cttcctttcg
     2281 taaacataat ggttgaaggc actacccagg gtgcagcatc agatattgat ggcttttatt
     2341 ccatcatcgg attaagacca ggcaattacg atgtgaaagc ttcagcaatt ggatataatt
     2401 cacaaacggt tacaggtgta cgcgtctcaa ttgatttgac aactgaagtt aattttcaat
     2461 tgaaggaaac cagcatcgag cttaaggaag aggtggttgt tattgctaca agacctctgg
     2521 taaccaaaga tctaacatcg tcaactgcta ttgtaagcgg tgatgatatt tccgttttac
     2581 ccgttacaga atttcaggaa gtattacagc ttaaagccgg aatagttggc ggtaacgtaa
     2641 gaggcggtag aagcggtgaa gttgtttatg caatagacgg cgtaccggtt accgacgtct
     2701 acgatggttc cacagttgtt gatgttgcca caagctctat tcaagaactg caatttgtaa
     2761 gcggtgcttt taatgctgag tacggaaaag cgttatcggg atatgttaac attgccacca
     2821 aagatggtga taataaattc cagggttctg ctactagcta cataggtgat tatgttagta
     2881 atcgaacaga tatttttaga gccattgatc aggttaatcc tacctctata agagattttg
     2941 aaggcagtat aagcggacca attataccga atcaattatt cttttatgtg aatgcaagat
     3001 atatttactt cggtggatgg ttgaatggcc aaaataatta taatccatgg aatataacta
     3061 ttaatcaggg agccacagtc ccgattgaaa acagatatat attgagcgcc aatcctgata
     3121 gtggattagg aaatggtgat attgtttcca tgaactggaa tgaaaaattg tatggtcagg
     3181 gaaaattaac ttacagacca ttttcaggag ttaaattaaa ctttaattat atatatgata
     3241 atgtggaata cagagatttt gatcaatcat tctcttacaa ccctgcaggt gattataaaa
     3301 agtttagagt tggaaataca aatatccttg gcttaaccca cacactatct cctacaactt
     3361 tctatcaagc aaatttttct tatttcttca agcaattcaa gcaatatgta tatgaagatg
     3421 taaatgatcc cagatggacc aacgatagat tgttaagcca ggaacctaca gaagtaccta
     3481 gttttagaac tggtggaact caaagccagc aatttaaacg caatacaagc acatatggat
     3541 tcaaattaga ttttacttct caggttgata agattaacat ggttaaattt ggaattgaag
     3601 ctaatcagca tattctggga tttgatttaa ttaatctgca gcagccttta tcggttcctg
     3661 atcctgctgt tacattaaac ccattcgtta caagatatgt ccctgatatt aatgatcccg
     3721 accaaaatct tgcaatagat atttatcaaa gaaaacccat cgaattatca gcttatctgc
     3781 aggataaaat tgaattaaat gaaataatta ttaacgtggg ttttagatta gattattttg
     3841 atcccgatgg acttgtttta aatgatatta cagatcctga tatatacaga ccaagaaaag
     3901 atgttaatat ctctaagact ctggaagaaa gaagagcctc taatgctttt ggtgatcctt
     3961 ggtacagaaa agcagataca gaaatgaagt tttcaccaag gcttggtatt gcttttccta
     4021 ttaccgatag aggagttata cacttttctt acggacattt tttccagata ccaaattttg
     4081 aattattgta tcagaatcct gaatttaaat ttggagccgg aacaggaaat cttggtatcg
     4141 caggtaattc caatcttaaa gctcagcaaa caattagtgg cgaagtagga gtatcccagg
     4201 cttttaccga tgatatcaca gtagatatta cggcttattt tagagatata agaaatcttg
     4261 ccggtacaag ggcagatgta atcagacttc cgggtggttc ttccagctac agccaatatg
     4321 taaacagcga ttttggattt gtgcagggaa ttatacttac gttaacaaaa agattatcca
     4381 ataactggtc ggcaacaatt gattatacat tccagtctgc aaaaggaaat gcttctgacc
     4441 ctgcggccac cagaaatcaa attgcaggcg gtgaacagcc ggaaataaaa ttgattaaat
     4501 taaactggga tcaaacaaac acggttaacg taacattttc ttatacttcc gatgataatt
     4561 ggggattcag tttaatcgga caatacggaa gcggattccc ttacacccca acgctatcac
     4621 aaaatgtatc tactttgcta atcaatagcg aattaaaacc aagctttaag aatgttgatc
     4681 ttagagctta taaagatttt ataattagt
//
LOCUS       DASWBY010000696         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_103824, whole genome shotgun sequence.
ACCESSION   DASWBY010000696 DASWBY010000000
VERSION     DASWBY010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103824"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..412
                     /locus_tag="VF870_12710"
                     /pseudo
     CDS             47..412
                     /locus_tag="VF870_12710"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
     gene            416..964
                     /locus_tag="VF870_12715"
     CDS             416..964
                     /locus_tag="VF870_12715"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253100.1"
                     /translation="MYFEQTGLPWVLSSPHVPHKFSPFYYTISGIIGELRGVLSIGVG
                     YTIPFQTFATEWINGEELAGKMNSYNLEGVIFRPITYQPYYGFGKDKMLHGVEVYITD
                     YKNINLMPIQFYFAQAVNELYGRNIISENEGRFDMFDKVLGTSKIRELFMQNLKVDDI
                     LPYLNKDVENFRTISKKYYLYN"
     gene            1070..2332
                     /gene="lysA"
                     /locus_tag="VF870_12720"
     CDS             1070..2332
                     /gene="lysA"
                     /locus_tag="VF870_12720"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX9253101.1"
                     /translation="MHYIESEYISYKKNKLYVEDTSVNDLAKKFETPLYIYSKNHFIK
                     QYKEFENAFKDINHKIFYAMKANFNLSVISTFVKLGSGVDANSEGELFRALKAGAEPL
                     NIILTSVGKTKNEIRLGLEKNVLMIKAESEEEIELINKIAGEMNKVARVAIRVNPDVD
                     AKTHPYISTGLSSNKFGVDSKTALSIYKRRKDFPNIQFTGIDMHIGSQITSIDPFVEA
                     VEKLSELYFEIEKDGLKLEHFDVGGGIGVSYNSEKTFSILDFAKRTIPLFKKLNCQII
                     FEPGRFLTANGGILVTEVLYNKRNGEKNFVVVDAAMNDLLRPSIYQAYHHVQPVQKFS
                     ERNEIVADVVGPICESGDYFARDREITVSNSGELLAVMSAGAYGMVMSSNYNARRRLA
                     EILVDGDKYSMIRSRETFDHLVWDEKIV"
     gene            complement(2446..>3027)
                     /locus_tag="VF870_12725"
     CDS             complement(2446..>3027)
                     /locus_tag="VF870_12725"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX9253102.1"
                     /translation="AHLLENDPAYAEKAKRFSSKVKDVMEFFAEQKPELNYGTLISME
                     SSTGKSDISNSQQISPSGRNDKLDNLEYTPELTSITYHDACHLIHAQKVSTQPREVIK
                     SLPGINYTELEEANWCCGSAGIYNVVRYDDAVKQLERKMNNIKNTKAKIVLTGNPGCM
                     GQIKHGTQKFNVDVEVLHPVTLIKRMLKSQQKP"
BASE COUNT          976 a          481 c          600 g          970 t
ORIGIN      
        1 tgtagatttt tatattgatg acgcaacaaa acttccagtc tattctttgt atggcaagac
       61 ccgcaaacca acttcagata tgttaaagga tgttgatgtt ttagtctttg atatccagga
      121 tattggaagc cgttcttaca cctatattag tacaatgggt ttggtaatgg aagcggcggc
      181 agaaaataat aaagaagttg ttattcttga tcgtccgaat ccgcttggtg gaaacagaat
      241 tgaaggcaat ttagttgaag atggatatta ttcttttgtc agtcagtttg caattcctta
      301 tgtgcatgga ttaactgtct gagagcttgc taaattatta aatgaagaag ggctgttaga
      361 aaataaagtt aaatgtaagc ttcaggttgt tccgatggaa ggatggacac gggaaatgta
      421 ctttgaacaa acaggattac cctgggtttt aagttcgccg cacgttccac ataagttctc
      481 tcctttttat tatacaatct ctggaataat tggtgagctg cgcggagttc tttcaatcgg
      541 tgttggttat acaatcccgt ttcaaacttt tgcgactgaa tggataaacg gtgaagagct
      601 tgcgggcaaa atgaattcat ataatctaga gggagtaatt tttagaccga taacttatca
      661 gccttattat ggatttggaa aagataagat gctacacggc gttgaagttt atattactga
      721 ttataaaaat attaatctga tgccgattca attttatttt gcacaagctg taaacgagtt
      781 atatggaagg aatatcattt ctgaaaacga aggaagattt gatatgttcg ataaagtttt
      841 aggtacgagt aagatcaggg aattgtttat gcaaaactta aaggttgatg atatacttcc
      901 ctacctcaat aaggatgttg aaaattttag aacgatttct aaaaaatatt atttgtataa
      961 ctagttcggt catgcttcga cttcgctcag catgacgatg gcatcaaaac taaattctgt
     1021 cacactgagc gtagttgaag tgtgactttc attaaacaga aatattatta tgcattacat
     1081 cgaatcagaa tacatcagct acaaaaaaaa taagctttat gttgaagata ccagcgtaaa
     1141 tgatttagct aaaaagtttg aaactccgct gtacatttac agcaaaaacc attttataaa
     1201 acagtacaaa gaatttgaga atgctttcaa ggatatcaac cataaaatat tctatgctat
     1261 gaaggctaac tttaatctca gtgtaattag tacatttgta aaactgggca gcggagttga
     1321 tgcaaactct gagggtgaac tatttcgcgc actgaaagcc ggtgctgaac ctttaaatat
     1381 tattctaaca agtgttggta aaacaaagaa tgaaattaga cttggattag aaaaaaatgt
     1441 tttgatgatt aaagctgaat ctgaagaaga aattgaattg ataaataaaa ttgccggtga
     1501 aatgaacaaa gttgcacggg ttgcaattcg tgttaaccct gatgttgatg caaaaaccca
     1561 tccatacatt tcaacaggtt tatcatcaaa caaatttggt gttgattcca aaaccgcttt
     1621 atcaatttac aagcgaagaa aagatttccc caatattcaa ttcactggaa ttgatatgca
     1681 catcggttcg cagataactt ccatcgatcc atttgttgaa gcggttgaaa aactttctga
     1741 actttatttt gagattgaaa aagacggatt aaaacttgag cattttgatg tcggcggcgg
     1801 aattggcgtt agttacaata gtgaaaaaac attttcaata cttgattttg cgaagagaac
     1861 aattcctttg ttcaaaaaac taaattgcca aataattttt gaaccgggca gatttttaac
     1921 tgctaatgga ggaattctgg tcacagaggt tttatacaat aaaagaaatg gtgaaaagaa
     1981 tttcgtagta gtcgatgccg caatgaatga tctgcttcgt ccatcaattt atcaggcgta
     2041 tcatcacgta caacctgttc aaaaatttag tgaaagaaat gaaatagttg cagatgtagt
     2101 tggtcctatt tgtgaatccg gcgattactt tgcacgagat agagaaatca ctgtatcaaa
     2161 ttcaggtgaa ttattagctg ttatgtctgc tggtgcttat ggaatggtga tgagttcaaa
     2221 ctacaatgca cgccgccgcc ttgccgagat tcttgtagat ggtgataaat attcaatgat
     2281 tagaagcaga gaaacatttg atcatttggt ttgggatgag aagatagttt gaactaatct
     2341 taattgtcat ttcgaacgaa gtgagaaatc tagaaggtaa attttaatag atttctcagc
     2401 ctccctttcc ggtaggcagg tcgaagactc cttcgaaatg acaaatcaag gtttttgctg
     2461 agacttcaac atccttttta tcaacgtaac cggatgtaga acttcaacat caacattaaa
     2521 cttttgagtg ccgtgcttta tttgccccat acaacctgga tttccggtta aaacgatttt
     2581 tgcttttgtg tttttgatgt tattcatttt tctttcaagc tgcttaactg catcatcata
     2641 acgaacaaca ttataaattc ccgcactgcc gcagcaccag tttgcttcct caagttctgt
     2701 gtaattaatt ccgggtaaag attttattac ttcccttggc tgggttgaaa ctttttgtgc
     2761 atgtattaaa tgacaagcat cgtgataagt aatggaagta agttcaggtg tatattcaag
     2821 attatccagt ttgtcatttc gaccggaggg agaaatctgt tgtgagtttg aaatatcaga
     2881 tttcccggtt gaggactcca tcgaaattag agtaccataa ttcaattcag gtttttgttc
     2941 tgcaaaaaat tccatcacat ccttaacttt agatgaaaag cgtttagctt tttctgcata
     3001 agcaggatca ttttcaagca gatgggc
//
LOCUS       DASWBY010000697         3600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_103878, whole genome shotgun sequence.
ACCESSION   DASWBY010000697 DASWBY010000000
VERSION     DASWBY010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103878"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF870_12730"
     CDS             <1..208
                     /locus_tag="VF870_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253103.1"
                     /translation="GSKVYKGQDFLGIVSDYFETPANPVIEINRDGVNNLLIPYVVSV
                     FEKIDLENKVLILNSDFGIDDDED"
     gene            198..872
                     /gene="trmD"
                     /locus_tag="VF870_12735"
     CDS             198..872
                     /gene="trmD"
                     /locus_tag="VF870_12735"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9253104.1"
                     /translation="MKIDIISAVPELLSSPLNTSILKRAQDKKKVKIEIHNLRDYAFN
                     KHKQIDDKPFGGGAGMVLKPEPFFECIEKLKSTCNYEHIIFTTPKGKLFDQRYANRLS
                     LAQNVLIIAGHYKEIDDRVREYFATDEISIGNYVLTGGELPALIIVDAIIRLIPGVLN
                     DSEAALDDSFQDGDVVEAPYYTRPAEYRGMKVPEVLLSGNEKEIKKWKQEQSKILTEK
                     WRNFNN"
     gene            885..1229
                     /gene="rplS"
                     /locus_tag="VF870_12740"
     CDS             885..1229
                     /gene="rplS"
                     /locus_tag="VF870_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9253105.1"
                     /translation="MIDLNKIAPDQIKTDMPDFNPGDHIRVQVRVIEGDKERLQSFEG
                     DVISIRGSGLNKTFLVRKMSSGVGVERIFPFNSPKIAKVEVLKQGDVRRAKLFYLRNL
                     SGKAARIKSKKQ"
     gene            1277..2038
                     /locus_tag="VF870_12745"
     CDS             1277..2038
                     /locus_tag="VF870_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253106.1"
                     /translation="MKTKLILPKNIFTKIFLSELNPDDSIAVEFQPAAVIAKKLTQEE
                     NSIGIIPTLDLLTYKDLFISSKMGVSFNALLSNSYIHFKEGQETLEQVFLKGDVTTNE
                     IILGKILFKEFYDVDVTPTLIKDENAHSNDNTLIVGDENYEKELFLNGLSFAEEIIEL
                     LDAPYVNFILAGSSEKSLKDFTDKYRDNLLNGHTEDYADLLKGITQTSLDFISVNMQH
                     IVFDLEDQDLEGIKSLLQLPYFHGIVKDMIDIKFV"
     gene            complement(2092..2991)
                     /locus_tag="VF870_12750"
     CDS             complement(2092..2991)
                     /locus_tag="VF870_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253107.1"
                     /translation="MKIIFMLIIVSGIIPSQFSFSQPLTERYNHLGEVFVPQFSSAPF
                     PHPERCNGHVYDDKTYSAQEHYSDSSVAIFIPKGFRQEEKINFVVYFHGWNNNIDSAC
                     AQFRLIEQFCESNINAIFVFPEGPKNSPDSFGGKLEEKDGLKKLIDDVLKYLNENNKL
                     NSEVIGNISLAGHSGAYRVISFCLMRGGLTPNISDVILFDALYGQTEKFSHWIENFNR
                     RFINIYTDNGGTKAETESLMDDLTAWQIPYFKTEESQLKIDDLKNNQLIFIHTDLTHN
                     EVISTRNQFRNYLQTSILPLIGQ"
     gene            complement(3048..>3600)
                     /locus_tag="VF870_12755"
     CDS             complement(3048..>3600)
                     /locus_tag="VF870_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9253108.1"
                     /translation="MFSYMKNGAYLVNTSRHDVIVEDDLLEAIKEKNIRYACDVFKGE
                     PEGKSGEVSSKLQNNPNIYVTHHIGASTEQAQDAVAEETINIIKKFVHSGVIDHWVNR
                     AKITDAHYQLVVKHYDKPGVLASVLDVIRSGHINIEEIENIIFEGGIAACCTLKLQSA
                     ATADMLIQISENTNVISVSHVEI"
BASE COUNT         1202 a          576 c          636 g         1186 t
ORIGIN      
        1 tgggagtaaa gtttacaaag gccaggattt tttaggaatt gttagtgatt attttgaaac
       61 tccggcaaat ccggttattg aaataaacag agatggtgtg aataatttgc tgattcctta
      121 tgttgtttcc gtttttgaaa aaattgatct tgagaataaa gttttgattt taaattctga
      181 ctttggtatt gatgatgatg aagattgata ttatttctgc ggtgcctgag cttctctcaa
      241 gtccgcttaa tacaagcatt cttaaaagag cgcaggacaa gaaaaaagtt aaaattgaaa
      301 ttcataatct tcgcgattat gcatttaata aacacaagca gattgatgat aaaccatttg
      361 gcggcggggc aggaatggtt cttaaaccgg aaccgttttt tgaatgtatt gaaaagttaa
      421 aaagtacatg caattatgag catataattt ttactactcc aaaagggaaa ttatttgatc
      481 agagatatgc aaacagatta tctcttgcac aaaatgttct gataattgca ggtcattaca
      541 aagagattga tgacagagtt agggaatatt ttgcaactga tgagatttca atcggaaatt
      601 atgttttaac tggtggtgaa cttccggcac ttattattgt agatgcgata ataaggttga
      661 tccctggagt attaaatgat agtgaggcag ctcttgatga ttcgttccag gatggtgatg
      721 tagttgaagc accatattat acaagacctg cagagtatag gggaatgaaa gtacctgaag
      781 tgttgctttc agggaatgaa aaagaaatta aaaaatggaa acaagaacaa tcaaaaatct
      841 taactgaaaa gtggagaaat tttaataatt agcggagtat agtaatgatt gatttaaata
      901 aaatagcacc tgatcagatt aaaactgata tgcctgattt taatcctggt gatcatattc
      961 gcgttcaggt tagagtaatt gaaggcgata aagaaagatt acaatctttt gaaggcgatg
     1021 taattagtat tcgcggctca ggattgaaca aaacattttt ggtaagaaaa atgtcgagcg
     1081 gtgttggcgt cgaaagaatt tttcctttca attcacctaa aattgcaaaa gtagaagtgc
     1141 ttaaacaggg tgatgttaga agagcaaaac ttttctatct caggaatctt tccggtaaag
     1201 ctgcaagaat taagagcaaa aaacaataaa actaaatatt ttaggaagcc gctagactca
     1261 gcggcttttt tattctatga agacaaaact tattctccct aaaaatattt ttacaaagat
     1321 tttcctatct gagttaaatc ctgatgatag tattgccgtt gagtttcagc ctgcagcagt
     1381 gattgctaaa aaattaacgc aggaagaaaa ctcaatcgga ataattccaa ctctggattt
     1441 acttacatat aaagatctat ttatatcatc aaaaatgggc gtatcattta atgcactact
     1501 ttcaaattca tatattcatt ttaaagaagg gcaggaaacc ttagagcagg tatttctaaa
     1561 aggtgatgtt acaacaaatg aaattattct gggtaaaatc ctctttaaag aattttatga
     1621 tgtggatgtt acaccaactt taattaaaga tgagaatgct catagcaatg ataacacact
     1681 tattgttggt gatgaaaatt atgaaaaaga attgttctta aatggattaa gttttgcgga
     1741 agaaataatt gaattgctag atgctcctta tgttaatttt attcttgcag gttcttctga
     1801 gaaatctctt aaagatttca ctgataagta tagagacaat ttacttaacg gccatacaga
     1861 agattacgca gatttactaa agggaattac tcaaacttca ctcgatttta tttcggtcaa
     1921 catgcaacat atagttttcg atttagaaga tcaggacctt gaaggaataa aatcattatt
     1981 acaattaccg tattttcatg ggattgtaaa ggatatgatt gacattaagt ttgtgtagca
     2041 gttttcaaac tgaaaatcaa atctcacgaa gcattcttaa accttaagaa ctcattgccc
     2101 gattaaaggc agaatacttg tctgtaaata attccgaaat tgatttcttg ttgatattac
     2161 ctcgttgtgg gttaggtcag tgtggataaa tatcagttga ttattcttta aatcatcaat
     2221 tttaagttgt gattcttcag ttttaaaata tggaatttgc catgcggtta aatcatccat
     2281 caagctttca gtttctgctt ttgtcccgcc attatctgta taaatattta tgaaacgtct
     2341 attaaaattt tctatccagt gagaaaattt ttctgtttgt ccgtaaagtg catcaaataa
     2401 gataacatct gaaatattag gagttagacc accgcgcatt aagcaaaatg aaataacacg
     2461 atatgcgccg ctgtgtcctg ctaaactaat atttccaatc acttcggaat ttaacttatt
     2521 attttcattc agatatttta gtacatcatc aatcaacttc ttcaaaccat ctttttcttc
     2581 caacttccct ccaaaagaat cgggagaatt ttttggccct tctggaaaaa caaagattgc
     2641 atttatatta ctctcacaaa attgctcaat aagtctaaac tgagcgcagg cactatcaat
     2701 gttattattc cacccatgga agtaaaccac aaaattaatt ttttcttcct gtctaaatcc
     2761 ttttggaata aatatagcaa cactgctatc gctataatgc tcttgggcag aatatgtttt
     2821 atcatcataa acatgtccat tacatctttc tggatgtgga aatggtgcag acgagaattg
     2881 tggaacaaac acctctccaa gatgattata tctttctgta agcggttggg agaatgaaaa
     2941 ttgggaaggg attatccctg aaacaataat tagcataaaa attattttca tggctgtacg
     3001 tttataatta aaaaatgggc gattcagcaa atcgccctta aataaaacta aatttctacg
     3061 tgactaacac taatcacatt tgtgttttca cttatctgta ttagcatatc tgctgttgca
     3121 gcagattgca attttagtgt acagcaggct gcaattccgc cttcaaaaat tatgttctcg
     3181 atttcttcga tattaatgtg tccgcttctt attacatcaa gcacgctcgc aagtacgccc
     3241 ggcttatcat aatgctttac aacaagctga taatgtgcat ctgttatctt tgctctgtta
     3301 acccaatgat caataactcc gctgtgaaca aatttcttaa tgatatttat tgtttcttcg
     3361 gcaactgcat cctgtgcctg ctcagtagaa gcaccaatat gatgagttac ataaatgttt
     3421 ggattgtttt gaagtttcga agaaacctca ccggactttc cttcaggttc acctttaaat
     3481 acgtcacagg cataacgaat atttttttct ttaattgctt caagcaaatc atcttccaca
     3541 ataacatcgt ggcgtgaagt atttaccaga taagcgccgt tcttcatata actaaacatt
//
LOCUS       DASWBY010000698         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_255966, whole genome shotgun sequence.
ACCESSION   DASWBY010000698 DASWBY010000000
VERSION     DASWBY010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255966"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..600)
                     /locus_tag="VF870_12760"
     CDS             complement(34..600)
                     /locus_tag="VF870_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HEX9253109.1"
                     /translation="MATDKIFAINDVLVRDEIVEIPFSCDLKKCKGACCTLESELGAP
                     VNKEEIDEIRKILPVVKKYLTQTNIDEIEEQGFYEIKDGEMMINSVNNHDCVFSFWDN
                     GIAKCAIERAYFDGKVEFRKPISCHLFPIRVSDFSGDVLRYEKFSECVPALEKGKADN
                     VTIAEFCKDSLTRLYGEEWYNKLMNYIR"
     gene            775..1545
                     /locus_tag="VF870_12765"
     CDS             775..1545
                     /locus_tag="VF870_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9253110.1"
                     /translation="MIFKNSVVLITGASSGIGYQLAKVLAAEGAKLALVSRRIELLDS
                     LAEQLKDQTVIHNYKCDVTNKVEVAETISKIKKDFGSIDIAILNSGVGYTASVLNYNS
                     IEAEKTFNTNVLGAVYCIEQLLPEFIAEKRGVIVGISSLGDGKGFPKSGFYSASKAAL
                     TIILESLRIELKKYKVKVITVKPGFVKTPMTDKNNFKMPFLMTVEEGSKIILNGLRKD
                     KRIIEFPWQTTVGAKVLRMMPTKWFEAIASKEPPSRKK"
BASE COUNT          521 a          274 c          260 g          543 t
ORIGIN      
        1 gtgtaagtct ttggctgagt gaaagcatat tgtctacctg atatagttca taagtttatt
       61 gtaccattct tccccgtata accttgttaa agaatcttta caaaattccg cgatagtaac
      121 gttatccgct ttaccttttt ctaatgccgg tacacattca ctgaattttt catatctcaa
      181 cacatcgccg ctaaagtctg aaactctgat gggaaaaaga tgacaggaga tcggttttct
      241 aaattcaact ttgccatcaa aatatgctct ttcaattgcg cactttgcaa ttccattatc
      301 ccagaaagaa aaaacgcaat catgattatt tacactatta atcatcattt cgccgtcttt
      361 aatttcatag aatccttgtt cctcaatttc atcaatatta gtctgtgtca gatatttttt
      421 tacaaccgga agaattttcc taatctcatc aatttcttct ttattaacag gtgcaccgag
      481 ctcgctttcc aatgtacagc acgcaccttt gcatttcttc aaatcgcagg aaaagggaat
      541 ttctactatc tcgtcccgga ctaaaacgtc atttattgca aatatcttat cagttgccat
      601 tggaataatt tttgctatta ttgtgccaaa atatacagta aaaattcttt ttatcgcaac
      661 ttgattttta attttattcc acactcctta aacaatctat aaagttttct tatcttttag
      721 ttgattttaa taattcaaat cgattatttg ccttatacta attccggatt ttttatgatt
      781 ttcaaaaact cagtagtttt aataaccggg gcttcttcag gcataggcta tcagcttgca
      841 aaagttcttg cagccgaggg agctaaactt gcattggttt ccaggagaat agaattgtta
      901 gattcattag ctgaacaatt aaaagatcaa acagttatcc ataactacaa atgtgatgtt
      961 accaataaag ttgaagttgc agaaacgatt tctaaaatca aaaaagattt tggcagtatt
     1021 gatattgcaa ttcttaactc gggtgttggc tacacagcaa gtgttttgaa ttataattct
     1081 attgaggcag aaaaaacttt caacacaaat gtgcttggag cagtctattg cattgagcag
     1141 cttcttcctg aatttattgc tgaaaagcgc ggagttattg tcgggatctc gagtctcgga
     1201 gacggaaaag gttttcctaa aagtggattt tattctgcaa gcaaagctgc gcttacaatt
     1261 attttagaaa gtttacgcat cgagcttaaa aaatataaag taaaagttat tacagttaaa
     1321 ccgggttttg ttaaaactcc tatgacagat aaaaataatt ttaaaatgcc cttcttaatg
     1381 actgttgagg aaggaagtaa aattatttta aatggattaa gaaaagataa gagaataatt
     1441 gagtttccgt ggcagacaac tgttggggca aaagtattgc gaatgatgcc cactaaatgg
     1501 tttgaagcaa ttgcttccaa agaaccgccg tcaagaaaaa aataaaatac ttttttagat
     1561 aaatatttat tgagtttaaa atgattcaga aaatattt
//
LOCUS       DASWBY010000699         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_292187, whole genome shotgun sequence.
ACCESSION   DASWBY010000699 DASWBY010000000
VERSION     DASWBY010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292187"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VF870_12770"
     CDS             complement(<1..1004)
                     /locus_tag="VF870_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253111.1"
                     /translation="MLKILHIAPLNFAGVPFDFYKMHNACGNHSRIITLHQNERVFPE
                     DICLNLPLPNQTLAKILRRKKIESLEEKERDTAHYFESRNLFEKSYYKIYDLLIQPRI
                     EKAISDYSLNDFDIIHYDGGLDFYRDSRQALKWKKQGKKIVCCYYGSDLRIRGLIKEL
                     DEISDLNITSEYDHLALKKDLKYLFYPYDASELPNKKERTGEALKIAHSPTNRKYKGT
                     ELIISVIDKIKKERKIEFLLMENVQRKKLLGIKSECDISIDQVGGSMGGTGYGKAGLE
                     TLAMGIPTITNMTKEYANWLPENPFVIANNFDELYKSLIELIDNELYRTEYGKKGIEW
                     V"
     gene            complement(1004..1975)
                     /locus_tag="VF870_12775"
     CDS             complement(1004..1975)
                     /locus_tag="VF870_12775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9253112.1"
                     /translation="MLEYFQFDNKKITSDQWDEFVEQSDNGTMFSKRKFLSYHAKDKF
                     NDKSIYITKDGKLHSVFTAALIERDGKKILSSHPGASYGGFVYNSELNFREAHDLVNI
                     MIEHAKSVKADRIQLTLAPIIYQTKYSNYLDFALVRNGFQYLKREISSVVQLDFEPDQ
                     LLNTYRAEARTATKKALKKGVEIVETERFDEYYKILKKNLKLRHGVNPAHTLEELKKV
                     KNLFPSKVRLWGAFLNNEMLAGVCNFSVNKNVVLAFYISHDEAFQEYRPVNLLFYEIM
                     KRYQAEGFKFLDFGIFTVIMEPNWGLGRFKENFGARGIFRDTFYKDI"
     gene            complement(2159..>3112)
                     /locus_tag="VF870_12780"
     CDS             complement(2159..>3112)
                     /locus_tag="VF870_12780"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9253113.1"
                     /translation="LELTLFFNKKNVSMKQFKIIKQFILFIIISTVASFGQSAGNTGL
                     SFLKYGFGARNIAMGDAGTALSNDLTGLFYNPARLAMTDKSEVLFMHNQWIQDVNSEV
                     IGAKTELWGIPFALGFNVTSIDGIEYREIPGEPVTTFDANYFYGSLSTGFFVTDEISF
                     GAGIKYLYEGLLNDEATGFGFDFGLNYLLPYKGLTASAVVKNIGSMNALRNEKTKLPT
                     ELRIGTSYTYNLEDSKFDFTGGLEYQKYLDTEDNHFNFGGEVLYNKLIAARAGYQTGY
                     ESRGFTAGIGLMWGNLKFDYAFLPFSYGLGNANLFSLQFKF"
BASE COUNT          995 a          578 c          471 g         1068 t
ORIGIN      
        1 acccactcaa ttcccttttt accatattca gttctgtaga gctcattatc aattaactca
       61 atcagggatt tgtacaattc atcaaaatta tttgcaatca caaatggatt ttcaggcagc
      121 cagtttgcat attcttttgt catattggta attgttggaa ttcccatagc aagagtttca
      181 agtcccgctt tgccgtatcc agttccaccc atagaaccgc caacctgatc aatggaaata
      241 tcgcattcgc tttttatccc gagcaatttt tttctttgca cattttccat caataaaaat
      301 tcaatctttc gttctttttt aattttgtca ataacagaaa ttattagttc agtgccctta
      361 tattttctgt tagttggcga atgggcaatt ttcagagctt caccagttct ctctttttta
      421 tttggaagtt cggaagcatc gtatggataa aataaatatt tcaaatcctt ttttaatgcg
      481 agatgatcat attcgctcgt aatattgaga tcagaaattt catccagttc ctttatcaat
      541 ccacgaattc ttaaatcact tccataatag cagcagacaa ttttctttcc ttgttttttc
      601 cacttcaatg cctgcctgga atcacgataa aaatctaatc cgccatcata atgaataata
      661 tcaaaatcat tcaatgaata atccgaaatt gctttttcga ttctaggttg aattaataaa
      721 tcgtaaatct tgtaataact cttttcaaac agatttcggg attcaaaata atgtgcggtg
      781 tctctttctt tctcttcaag agattcaatt ttttttctgc gaaggatttt tgctagggtt
      841 tgattgggta atggaaggtt taaacaaata tcctcaggaa aaactctttc attttgatga
      901 agagtgataa ttcgtgaatg atttccgcaa gcattatgca ttttgtaaaa atcaaaaggc
      961 acaccggcaa agttaagcgg cgcgatgtga agaattttta gcattaaata tctttataaa
     1021 aagtatctct aaaaattcca cgtgcaccaa aattttcttt gaatctgccc aaaccccaat
     1081 ttggctccat aataacagta aatattccga agtctaaaaa tttaaatcct tcagcttgat
     1141 atcttttcat tatctcgtag aacagtaaat tgaccggacg gtattcctga aatgcctcat
     1201 cgtgacttat gtagaatgca agcacaacat ttttgttaac agaaaagtta caaaccccgg
     1261 ctagcatttc gttgtttaag aatgctcccc ataatcttac tttagatgga aataaatttt
     1321 taactttctt cagttcctct aaagtgtgag caggatttac accatgtctt aactttaaat
     1381 tctttttaag gattttataa tattcatcaa atctttcggt ctcaactatt tcgactcctt
     1441 ttttaagtgc tttttttgtt gctgttcttg cctcagcacg atacgtgtta agcagttgat
     1501 caggttcaaa atctaattga acgacacttg atatttctct ttttaaatat tgaaatccat
     1561 ttcgcactaa agcaaaatca agatagttgg aatattttgt ttgataaata ataggggcta
     1621 gtgttaactg aatgcgatca gccttaactg attttgcgtg ctcaatcatt atattaacaa
     1681 gatcgtgagc ttcgcgaaaa ttcaattcgc tgttataaac aaatccgccg taagaagcac
     1741 cgggatgcga tgataatatt ttctttccat cacgttcaat taaagcagct gtaaaaacag
     1801 aatgaagttt cccatccttt gtgatgtaaa tcgatttatc attgaattta tcttttgcgt
     1861 gatacgataa gaattttctt ttactgaaca tagttccgtt atcggactgc tcaacaaatt
     1921 catcccactg atcagaggta atttttttgt tatcgaattg aaagtattct aacataattg
     1981 tatctgttta atgtgaattt attttaacgg attaaaaata aggttatgat agtagatata
     2041 aaactgggga taaaaaaagg acacgattgc tcgtacataa aaatatttta agatattcac
     2101 acttcctccc tcgactgcgc tcgggatgac agttcagtga gataataaag ttacttcatt
     2161 aaaacttaaa ctgaagtgaa aacaagtttg cattgcccaa tccataagag aatggaagga
     2221 atgcgtaatc aaactttaaa ttgccccaca tcaaaccaat gcctgcggta aaaccacgac
     2281 tttcatatcc cgtttgatag cctgcacggg cagcaattaa tttgttgtac aaaacctctc
     2341 caccaaagtt aaagtggttg tcttcagtgt ctaaatattt ttggtattca agtccgcctg
     2401 taaaatcaaa tttagaatct tctaaattgt aagtataaga tgttcctatt cttaattctg
     2461 taggaagttt tgttttttcg tttctaagag cattcattga gccaatattt ttcacaacag
     2521 ccgatgctgt taaacctttg taaggaagta agtagtttaa tccaaaatca aaaccaaagc
     2581 ctgtagcttc atcatttaaa agaccttcat ataaatattt aatgcctgca ccaaaggaaa
     2641 tttcatcagt tacaaaaaat ccagttgata aacttccata aaaatagtta gcatcaaatg
     2701 ttgtaactgg ctcaccggga atttctctgt attcaattcc atctatagat gttacattaa
     2761 aaccaagtgc gaatggaatg ccccacaact ctgttttagc tccgataact tcactgttaa
     2821 catcctgaat ccactgattg tgcataaaca aaacttctga tttatcggtc attgctaacc
     2881 tggcaggatt ataaaacaat ccggtaagat cgtttgatag cgcagttcct gcatctccca
     2941 tagcaatatt acgagcacca aatccgtatt tcagaaatga taaccctgtg ttaccggctg
     3001 attgcccaaa tgatgcaaca gttgaaataa taataaataa aataaactgc ttaataattt
     3061 taaattgttt cattgataca ttttttttgt taaaaaataa tgttaactcc aa
//
LOCUS       DASWBY010000700         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_179728, whole genome shotgun sequence.
ACCESSION   DASWBY010000700 DASWBY010000000
VERSION     DASWBY010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179728"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VF870_12785"
     CDS             <1..1097
                     /locus_tag="VF870_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253114.1"
                     /translation="YNDPNIIYAESQNGGLGKSTNGGSGWVGATNGINGSESTNWSTP
                     VVMDPNSSNILYYGTHSLYRTTNGAASWTKISPQLTDWIPGRRLGTITTIAVAPTNSN
                     VIYVGTDDAHVWVSSDNGINWMDVSAGLPERWVTRVVVDPGDENIIYATFSGLKWRDP
                     QPHVFRSTNKGIDWTDISGNLPDAPVNAFAVDNNYSNRLYLGSDVGMYVSFDTGQSWE
                     VLGEGLPVLPVGDIKIHPTENFLAAGTYGRSMYKIDLNLVTDVMGQKPQIVKEFSLLQ
                     NYPNPFNPSTKISWQSPAAGYLALKVYDVTGKEIATLVDEYKSAGKYEIKFDASNLSS
                     GVYLYKLSARNSSLGSEHVYSEVKKMLVLK"
     gene            1163..>2109
                     /locus_tag="VF870_12790"
     CDS             1163..>2109
                     /locus_tag="VF870_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253115.1"
                     /translation="MSVKIHLSVFLLILISFTVSAQKRAFTIEDLYKVKNVSAPVLSN
                     SGEKIAFTVSESDLAKGKTTITVYVMSTNGSSLISISEKLPGATSPFWSSSDELYLIN
                     KGQVYKYSFETNDAVQITDFYAGVSNPVLSSDERYIAFTAELFPECGTDNDCNKGLDE
                     SSKNGPMQAYIADELLFRHWTDYKGEKESFLVLYDIKEKKYQAVTSSDVLSDTYMLGG
                     GPKYAFSSDSRVLAYVSSPEKNLANSTNTDIYLMPLGGTQNVNITLANDAWDGAPTFS
                     PDGNYIAYRTQLIPGFEADRFRVAVFNTKTLQSKVLTED"
BASE COUNT          677 a          373 c          424 g          635 t
ORIGIN      
        1 attataacga tccgaatata atttatgctg aatctcaaaa cggcggactt ggaaaatcaa
       61 caaacggtgg ctctggctgg gtaggggcaa ctaatggaat taacggcagt gagtcaacaa
      121 actggtcaac tccggttgta atggatccaa actcttcaaa cattttatat tatggaacac
      181 actcactgta cagaactaca aatggagccg caagctggac aaagattagt ccgcagttaa
      241 ctgattggat tccgggtaga agacttggaa ctattacaac tattgccgtc gctccaacaa
      301 attctaatgt catttatgtt ggaactgatg atgctcacgt ttgggtgtct tcagataatg
      361 gaattaattg gatggatgtt tccgcagggt taccagagcg ttgggttacg cgcgttgttg
      421 ttgatccagg tgatgaaaat attatttacg caacgttcag cggattgaaa tggcgcgacc
      481 cacagccgca cgtttttaga agcacaaata aaggaatcga ttggaccgat attagcggta
      541 atcttccgga tgccccggtt aatgcgtttg ctgtagataa taattattca aatcgtcttt
      601 accttggaag tgatgttggt atgtatgtta gctttgatac aggacaaagt tgggaagttc
      661 ttggtgaagg actgccggtt cttccggttg gtgatattaa aattcatcca acagaaaatt
      721 ttcttgctgc gggaacttac ggcagatcta tgtataaaat tgatctcaat cttgttaccg
      781 atgtaatggg ccaaaagccg caaatagtaa aagaattttc tttgttacag aattatccaa
      841 atccatttaa cccaagtaca aaaataagtt ggcaatctcc tgctgccggt tatctcgccc
      901 taaaagtata tgatgtaacc ggaaaagaaa ttgcgacttt ggttgatgaa tataagtctg
      961 ccggcaaata cgaaataaaa tttgatgctt caaatttatc cagcggagta tatttgtata
     1021 aacttagtgc ccgtaattct tctttaggtt cagaacatgt ttattctgaa gtaaaaaaga
     1081 tgttagtttt aaaataatcc atttgtaact tttaggtata aaatattatt atagtgattt
     1141 tcaagtaatt aaggagactg ttatgtctgt aaaaatacac ttatctgttt ttctgctaat
     1201 tctaatttca tttactgtga gcgctcagaa acgcgcattt acaattgaag acctttataa
     1261 agttaaaaat gtaagtgcac ccgtgctttc aaactcagga gaaaaaatag catttacagt
     1321 atctgaatct gacttagcaa aaggcaaaac aaccataacc gtttatgtga tgagcacaaa
     1381 tggatcttca ttaattagta tttcagaaaa actgccgggc gcaacttcgc ctttctggtc
     1441 atcaagcgat gaactttact tgataaataa aggacaggtt tacaaatatt catttgaaac
     1501 aaatgatgct gttcagataa ctgattttta tgcgggggtt tctaatcctg ttctttccag
     1561 cgatgaaaga tacattgcat ttactgctga actttttcct gagtgcggaa cagataatga
     1621 ttgcaataaa gggctagatg aatcttctaa aaacggacca atgcaggctt acattgcgga
     1681 tgaacttctc tttagacatt ggactgatta caaaggggaa aaagaatctt ttcttgtcct
     1741 ttatgatata aaagaaaaaa aatatcaagc tgtaaccagc agtgatgttt tatcagacac
     1801 atatatgttg ggtggtggac ccaaatatgc tttctcatca gacagcagag ttttagcgta
     1861 tgtttcgtcc cctgaaaaaa acctggcaaa ctccactaat accgacattt acttaatgcc
     1921 tttaggaggc acgcaaaatg taaatataac tcttgcaaat gatgcgtggg atggtgcgcc
     1981 aactttttca ccagacggaa actatattgc ataccgaact cagttaatac caggatttga
     2041 agcggacaga ttccgagtcg ctgtattcaa cacaaaaact ttgcaatcca aagtattaac
     2101 agaagattt
//
LOCUS       DASWBY010000701         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_104082, whole genome shotgun sequence.
ACCESSION   DASWBY010000701 DASWBY010000000
VERSION     DASWBY010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104082"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VF870_12795"
     CDS             <1..1396
                     /locus_tag="VF870_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9253116.1"
                     /translation="DTNERFHYLLSHGQTGLSVAFDLPTLMGWDADATISDGEVGICG
                     VAISSLKDMEILFDKIPLDKVSTSMTINSPAAMIFAFYLAVAQKQGVDFKNLRGTLQN
                     DILKEYIAQKEFIYPPNPSMRIIVDMIEYCKNEVPQWNPVSVSGYHIREAGSTSVQEL
                     AYTLADGFAYIEACIERGMDVDEFAPRISFFFNSHLDFFEEIAKFRAARKIYAKRMRE
                     KYGAKNPRSWWLRFHTQTAGCTLTAQQPENNIIRTAFQALAGVLGGTQSLHTNSMDET
                     LALPSEKAVKIALRTQQLIAYETGVINSVDPLGGSYFVEALTDKMEKEANAIFDQIDS
                     LGGVVAAIETGYFQKEIADAAYRYQKEVERKEKFIVGVNEFVEDNEKVDIPILTVSPE
                     VQKQQVKRLAELRQSRNQKAVEASLASIKSAAADGRNLMPEFLKAAQNYVTLGEMIGE
                     LKEVFGTYEETAVF"
     gene            1396..2274
                     /locus_tag="VF870_12800"
     CDS             1396..2274
                     /locus_tag="VF870_12800"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX9253117.1"
                     /translation="MIKNYFQLLRVPQWIKNLFVFVPLMFSLHLFEADYFITTLKAFI
                     IFCLASSFIYIINDIIDIEADAAHPKKKYRPLPSGKISKQSAWVTSVLIFVILIVLSF
                     ISPVEFIYFLAGFIILNITYSIYFKHIVILDIFSIAAGFTIRVLAGAAIINVPVSSWL
                     ILTTMFISLFLGVMKRHSELEQITESETTSTRKVLAEYSLTFTNQMATVAAAGVIICY
                     ALYTVSQRTVSVFGTENLIYTTPFVVFGIFRFMYLEYLNQKGENTTQIMLTDVPMIIT
                     VILYIATTVSIVYKIF"
BASE COUNT          771 a          381 c          442 g          769 t
ORIGIN      
        1 agatacgaat gaaagatttc attatttatt aagtcatgga caaacaggtt tatcagtcgc
       61 atttgatctt ccaacattaa tgggttggga tgccgatgct acaattagcg atggtgaagt
      121 aggaatttgc ggcgtagcga tttcatcttt aaaggatatg gaaattcttt ttgataaaat
      181 tccattagat aaagtttcga catcaatgac aatcaattca cctgcggcaa tgatttttgc
      241 attttatctt gcagttgccc aaaagcaggg agttgatttc aaaaatttac gcggcacact
      301 tcagaatgat attcttaaag aatatatcgc tcaaaaagag tttatctatc ctccaaatcc
      361 ttcaatgagg attattgttg atatgattga atattgtaaa aatgaagtgc ctcaatggaa
      421 tcctgtttct gtcagcggat atcatattcg cgaagcaggt tcaacatcag ttcaggaact
      481 tgcatacaca cttgcagatg gatttgcata tatcgaagcc tgcattgaac ggggaatgga
      541 tgtagatgaa tttgcacctc gaatttcttt cttctttaat tctcatttag atttttttga
      601 agagattgca aaattccgag ccgcaagaaa aatttatgca aagcgaatga gagaaaaata
      661 cggtgctaaa aatccgcgct catggtggtt aaggtttcac acgcaaacag ctggatgtac
      721 cttgactgct cagcagcctg aaaacaatat tatacgcact gcattccagg ccttggccgg
      781 agtgcttggc ggaacgcaat cacttcacac aaattcaatg gatgaaacct tagcattgcc
      841 gagtgaaaaa gctgttaaaa ttgctttaag aacacagcag ttgattgcgt atgaaacagg
      901 agtgattaat tctgttgatc ctcttggtgg aagttatttt gtggaagcac ttactgataa
      961 aatggaaaaa gaggctaatg caatttttga tcagatagat tcgcttggcg gagttgtagc
     1021 agcgattgaa accggttact tccaaaaaga aatagctgat gcagcttacc gatatcaaaa
     1081 ggaagttgag agaaaagaga agtttattgt tggcgttaat gagtttgttg aagataatga
     1141 aaaagtggat atcccgattc ttacagtttc tcctgaagta caaaagcagc aagtaaaaag
     1201 attagctgag ttaaggcaaa gcagaaatca aaaagcagtt gaagcaagtc tggcttcaat
     1261 aaaatcagct gccgcagatg gaagaaatct tatgcctgaa tttttaaaag ccgcacaaaa
     1321 ctatgttaca cttggcgaaa tgataggaga gttaaaagaa gtttttggta cttacgagga
     1381 aacagcagta ttctaatgat taaaaattat ttccaattat tgcgtgttcc acaatggatt
     1441 aaaaatcttt ttgtttttgt tccattaatg ttttccctgc atctttttga agctgattat
     1501 tttataacaa cgttaaaagc atttataata ttttgtctgg cgtcaagttt tatttatatc
     1561 attaacgata taattgatat tgaagcagat gcagcgcatc cgaaaaagaa atatagacca
     1621 ctgccatcag ggaaaatatc taaacagtca gcctgggtaa catcagtatt aatttttgtg
     1681 attttgatag tgctttcttt tatttcaccc gttgaattta tttactttct tgctggtttt
     1741 attattctaa atattactta ctcaatttac tttaaacata ttgtgatact tgacattttc
     1801 agcattgctg caggatttac aattagggtt ttagctggtg cagcaattat taatgttcct
     1861 gtttcgagct ggttaatatt aacaactatg tttatttcat tattccttgg tgttatgaaa
     1921 agacattctg aacttgaaca aattacagaa tcagaaacta cttcaacaag aaaagtttta
     1981 gctgaatact cacttacttt tacaaatcag atggcaactg ttgccgcagc aggagttata
     2041 atttgttatg cactttacac agtttcgcaa agaactgttt cagtattcgg taccgagaat
     2101 ctaatttata caactccatt tgtcgttttc ggaattttca gatttatgta tctggaatac
     2161 cttaatcaaa aaggtgaaaa cacaacacaa ataatgttaa cagacgtacc gatgattatt
     2221 acagttattc tttatatcgc tacaacagtg tccattgttt acaagatatt ttgataaaaa
     2281 aatgtaccag ctaaagatta tatcggtatt aatattttat aatttgatgt ttgcatcctg
     2341 tacatcttcg caacttcagt caa
//
LOCUS       DASWBY010000702         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_65232, whole genome shotgun sequence.
ACCESSION   DASWBY010000702 DASWBY010000000
VERSION     DASWBY010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65232"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VF870_12805"
     CDS             <1..677
                     /locus_tag="VF870_12805"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9253118.1"
                     /translation="LEIVPPGSWEKFDDLILSQEKIDFIVFTSAHAVTMFIKRTNELK
                     IQIDFERIKVVAVGNKTKSVCEENNIKVDILPKKFSGEGIVEELSKHDLKDRLIFIPR
                     SAIGREDLPKGLEELGAKITAVPVYNVSLPSKESVQHNVDKLNSSQPDVFIFTSPSTF
                     ENFLIIMNINNPVSFFKNYDVAAIGPTTKAAIENSKVKVSIMPDEFTIKGLANKMVEF
                     YNKIEK"
     gene            689..1762
                     /gene="hemE"
                     /locus_tag="VF870_12810"
     CDS             689..1762
                     /gene="hemE"
                     /locus_tag="VF870_12810"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX9253119.1"
                     /translation="MSELKNDLFLRACKRQDVERTPIWIMRQAGRYLPEYRAVRSKAD
                     FLTMCKTPELAAEVTIQPVDLIGVDAAILFSDILVIPEAMGMHLEMNEGKGPVFPNPI
                     RTVDDAKKLKDIDPTKELKYVLDAVSLTKKNLDNRVPLIGFSGSPWTLLTYMVEGKGS
                     KNFSEVKKLIYNNPGLAHSILDKLANTVADYLSAKIEAGCNAVQIFDTWGGILSQKDF
                     EEFSLRYVEKIISKIKRKNEPVIFFAKGVHYNLSKLASIGADVLGLDWTMDLGKVRRE
                     VGDKVALQGNLDPTVLYANKNYIKEAVKDILKSFGEGNGHVFNLGHGVLPDIDAENVK
                     ALVQYVKEESISFHRGDAEALRN"
     gene            1759..2145
                     /locus_tag="VF870_12815"
     CDS             1759..2145
                     /locus_tag="VF870_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX9253120.1"
                     /translation="MNSEQLNKLGAIILDASIEVHKQLGPGLLESVYELSLFKEFSAR
                     NLNVKKQVPIPVLYKGEDLNAEFKIDILVENEIVIELKAVEILLPVHKAQLLTYLRLS
                     EKKLGYLINFNVPKLIDGFNRIVNNF"
     gene            2187..>2611
                     /locus_tag="VF870_12820"
     CDS             2187..>2611
                     /locus_tag="VF870_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEX9253121.1"
                     /translation="MFEIDLTKFKKYDKPGPRYTSYPTAPQFNEGFTHEDYLNEIVKT
                     NYGKDLPDLSLYFHLPYCDTLCYFCGCNMIITRNRDRIKEYIKYLKKEIDLTRTYLLT
                     DRKVAQHHWGGGTPTHLNPDEINELASYINQSFNFKENA"
BASE COUNT          916 a          394 c          510 g          791 t
ORIGIN      
        1 cattagagat tgttccacca ggcagttggg aaaagtttga tgatttaatt ttgtctcagg
       61 agaaaattga ttttatagtc ttcacttctg cacatgcggt tacaatgttt attaagagaa
      121 ctaatgaatt aaaaattcaa attgattttg agagaataaa agttgttgcg gtaggaaata
      181 aaacaaaatc cgtatgtgaa gaaaacaata tcaaagttga tatcttacct aaaaagtttt
      241 ctggcgaggg gattgttgaa gaattatcta aacacgattt aaaagatagg cttatcttca
      301 ttccacgttc tgcaatcgga agagaagatt tgccaaaagg tcttgaagaa cttggagcaa
      361 aaattacagc tgttcctgtt tataatgttt cacttccgtc aaaagaatct gtgcagcata
      421 atgttgataa attaaattca agccaaccgg atgtttttat ttttacaagt ccttctacat
      481 tcgaaaactt tttaataata atgaacataa acaatccggt ttcatttttt aagaattatg
      541 atgtagcagc aattggccca acaacaaaag ctgcaattga aaatagtaaa gttaaagtaa
      601 gcataatgcc ggatgaattt acaattaaag gtctggccaa taagatggtt gagttttata
      661 ataaaataga aaaataacgg agaagatttt gagcgaacta aaaaacgatt tatttcttag
      721 agcttgtaaa aggcaggatg ttgagagaac accaatctgg attatgcggc aggctggaag
      781 atacctgccg gaatatcggg ccgtacgttc taaagcagat tttttaacga tgtgcaaaac
      841 cccggaacta gctgccgaag ttacgattca gccggtagat ttaataggag tagatgcggc
      901 aattctgttt tcagatattc ttgttattcc ggaggcgatg ggaatgcatc ttgagatgaa
      961 tgaagggaaa gggccggtat ttccgaatcc tattcgtacg gttgatgatg caaagaagtt
     1021 aaaagatatt gatccaacta aagaattaaa atatgttctt gatgcagtat cattgacaaa
     1081 aaagaatctt gataatcgag ttccactaat tggatttagc ggttcaccct ggacattgct
     1141 tacatatatg gttgaaggaa aaggctcaaa gaatttttct gaggtaaaaa aacttattta
     1201 taataatccc ggtttggcac attcaatact tgataaatta gcaaacaccg ttgctgatta
     1261 tctttctgcc aagattgaag ccggatgtaa tgctgttcag atttttgata cttggggcgg
     1321 tatactttct caaaaagatt ttgaggagtt ttctttacga tacgttgaga agataatttc
     1381 taaaataaaa agaaaaaatg aaccggtaat tttctttgct aaaggagttc attataatct
     1441 tagcaagttg gcaagcattg gcgctgatgt tcttggcctg gattggacga tggatcttgg
     1501 gaaagtcaga agggaagtgg gcgataaagt tgcactccag ggtaatcttg atccaactgt
     1561 tctttatgca aacaaaaatt atattaaaga agcagtaaag gatattctca aatcttttgg
     1621 tgaaggcaat ggacatgttt ttaatcttgg tcatggtgtg ctgccagata tcgatgcaga
     1681 aaatgtaaaa gcacttgttc aatatgtaaa agaagaaagc atttcttttc accgcggaga
     1741 cgcagaggcg ctgagaaatt gaatagtgaa caattaaata aacttggtgc tattattctt
     1801 gacgcttcaa ttgaagttca caaacaactt ggacctggtc tacttgaaag tgtttatgag
     1861 ttatcactat ttaaagagtt ctcagcaaga aatctgaatg ttaaaaagca agtcccaatt
     1921 cctgttttat acaaaggtga agatctaaat gctgaattta aaatagatat tcttgtagag
     1981 aacgaaattg ttatcgagtt aaaagctgtt gaaatattat tgcctgttca caaagctcaa
     2041 cttttaactt atctaagatt atctgagaaa aaactcggat atctaattaa ctttaatgtt
     2101 cccaaattaa tagatggatt taatagaata gtaaataatt tttaactctg cgtctcagcg
     2161 actcagcggt tagaaaagga ttaaatatgt ttgaaataga tttaacaaaa tttaaaaagt
     2221 acgataagcc gggaccacgc tatacaagct atccaacggc gccacagttt aatgaaggtt
     2281 ttactcacga agattatctg aatgaaattg taaagaccaa ttacggtaaa gatcttccgg
     2341 atctttcgct ttactttcat ttaccctact gcgatacact ttgttacttc tgcggatgta
     2401 atatgattat tactcgtaat cgcgaccgaa taaaagaata cataaagtat cttaaaaaag
     2461 aaattgatct tacaagaaca tatttattaa ccgatagaaa agtcgcacaa catcattggg
     2521 gcggaggaac accgacacac ctgaatcctg atgaaataaa cgagttagca tcatacataa
     2581 atcaatcatt caattttaaa gagaatgcag a
//
LOCUS       DASWBY010000703         6999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_256248, whole genome shotgun sequence.
ACCESSION   DASWBY010000703 DASWBY010000000
VERSION     DASWBY010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256248"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1226)
                     /gene="tsaD"
                     /locus_tag="VF870_12825"
     CDS             complement(201..1226)
                     /gene="tsaD"
                     /locus_tag="VF870_12825"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX9253122.1"
                     /translation="MIVLGIESSCDETSVAIIKDNELTTNLIASQDFHTNYGGVVPEL
                     SSRAHLQIVNPLLKDALKKSNIALSDVDLIAATAGPGLIGALLVGLTYAKGLAYGLNK
                     PFLGVNHIEGHIFSGFLMPQKPEFPFLSLVVSGGHTLLLLVNSFTEIYKLGSTVDDAA
                     GEAFDKVSKLLGLGYPGGPKIQNAAKLGDGSKIDFPVSDLKQKLNFSFSGLKTAVLRH
                     VQSQGGVDKLSLQDKNDIAASFQVAAVKALTRNVKRALENFDVKSISVVGGVAANAHL
                     KSEITKLAEKFKKNIVIPDLQFCGDNAAMIAFRAKSLFENGVRDNLSCKPFPALSENH
                     FLVIDQV"
     gene            complement(1223..2128)
                     /locus_tag="VF870_12830"
     CDS             complement(1223..2128)
                     /locus_tag="VF870_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX9253123.1"
                     /translation="MDAFNNLIVRFVQLMPKPVVGFFSKKYIAGETLQDAVDFVKKLN
                     SKGIYATMDVLGESVANKSESIKCKNEALEVLEAIEKNKLMANLSIKPTQMGLAIDEQ
                     FAYEQILELVKKAAEYKNFVRIDMEDSPFTDKTINLYKKIYADYKNVGVVIQAYMRRS
                     FDDVVELNKIGTNYRLCKGIYVEPAAIAYKGKQEVRDNYLKILDQMFKDGNYVGIATH
                     DKPLIDAAYTRITEQNISKDKFEFQMLLGVREDLRDKINSDGYKIRIYVPFGKDWYAY
                     SVRRLKENPSVAGTIAKSFLTFGRR"
     gene            complement(2307..2888)
                     /gene="coaE"
                     /locus_tag="VF870_12835"
     CDS             complement(2307..2888)
                     /gene="coaE"
                     /locus_tag="VF870_12835"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX9253124.1"
                     /translation="MKIGITGNIGSGKSTFTKFAEEAKYSILRADEISKDILSNDKVV
                     RELIIKEFGKQSFKDGKPDVRYLAKEVFSDPIKLSIIESILHPRVINSIEQSINTIEK
                     TDKVVFVEAALIYEADMEDMFDFVVLITAQRDLRLARKIASGITEDDFCKREANQIKE
                     EEKKKRADFIFSNETSFDDLKMKFNLLLLTLGI"
     gene            complement(3010..4572)
                     /gene="rny"
                     /locus_tag="VF870_12840"
     CDS             complement(3010..4572)
                     /gene="rny"
                     /locus_tag="VF870_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX9253125.1"
                     /translation="MDLIIIIPMIVVLIVLFFYLGWMFNSKVGKKSIVAAEEKAEQLI
                     QDAKKEAANLKREKLLEVKDEWYKKKIEFDTEINQKKQKLSNLEKQIQTREENSEKKF
                     DLVLQKEKELRKLEKQLQDLKVSLDEKSVEIKKLEVEQNDKLEKISGLTSEEAKKMLA
                     ENMINQAKIDASQTIKEIHDRAKVDAKKEAQKIIVQAIQRTAADYSIETTVSVVQIQN
                     DEMKGRIIGKEGRNIRAFESATGVDVIVDDTPEAVILSSFDQFRREIARISLERLISD
                     GRIHPARIEEVVEKVSAELEEEIQREGENTILQLGLHNMHGELVKHIGKMKYRSSYGQ
                     NLLQHSVEVAYLTGIMAAELGLDPVMAKRAGLLHDVGKTVDKSIEGPHALLGYDLTKK
                     YKEHAIVINAVGSHHEDIEMEHPIAALVQSADAISGARPGARREPLESYVKRLENLEL
                     LAKSFEGVAKTYAIQAGREVRVVVEHDKVDDASADRLAREIAQKIEEEMDYPGQIKVV
                     VIREVRKIGYAK"
     gene            complement(4803..5123)
                     /locus_tag="VF870_12845"
     CDS             complement(4803..5123)
                     /locus_tag="VF870_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX9253126.1"
                     /translation="MFDGVDNSMTDKKKLKIKIFDKEYSLLVENEEIAKELAIYVNKV
                     MEETKDELPDQPAQTIAIIACLNIAYDLFIEKNKNREFLIQATDKIKRIKLLLKEPEM
                     SDPS"
     gene            complement(5148..5459)
                     /locus_tag="VF870_12850"
     CDS             complement(5148..5459)
                     /locus_tag="VF870_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253127.1"
                     /translation="MVDLAKYDSIIHDLSAIEAEIATLGNKLRDTEERKTELEIQVAK
                     LRQDNSLLQKKVIELEQEILLSKQDEEGNIFNTLNSKEKENLKLKLQDIISKIDFHLS
                     S"
     gene            complement(5460..>6999)
                     /gene="pheT"
                     /locus_tag="VF870_12855"
     CDS             complement(5460..>6999)
                     /gene="pheT"
                     /locus_tag="VF870_12855"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9253128.1"
                     /translation="FTTLDSKERKLPVNTLMICDGNREVAIAGVMGGENSEITDTTNN
                     ILIESAYFNPSSIRKTSKTLGLSTDASYRFERGTDPNITKYAAERCAQLVAELADGKV
                     AKGIIDVYPNVILPKEIELRFSRTTRILGYEVPSEKIKNILSRLGLAVKVLDQDKLLV
                     SIPTFRPDIEREIDLIEEIARINGYDNIPTVSKINITLEKKTDETEIDEKIRQASTAL
                     GFFEMINNPLQSENDARLTGSPIKIANPLSADMECLRTSLLSGALITISKNIRQGVRE
                     LKLFEIGDSFIKISEGEIKTFSDFSETQNLLFAISGKDQIKGWNTDEKESDFYLLKGL
                     VDSFLSKISLDNVLIDSYYSTANEIYAYYLTKSFKEVELGRGGKLRQEVLKQFDINQD
                     VYCFEFDLSALKSIESISKTYTDPLKYPKIIRDFAFILDKSIKYLDVKEFIRKKSSQL
                     LKVISVFDVFESDSLGKDRKSIAFTLEYYDDNRTLTEDEVEKDFNNLIALITKEFNAQ
                     LRGK"
BASE COUNT         2140 a         1317 c          989 g         2553 t
ORIGIN      
        1 ctgcaattaa agcccactga agtttattaa atatgttagg aatttttact gacaattttt
       61 tatttattaa aaatatgagc gtttacaatt atggggtaca atataaaggc attgagtgaa
      121 aataattaac caaagtttta gcaagttatt cagttatttt aattctctcc aaatctcatc
      181 caaaatctga aattaatgat ttacacctgg tctatcacca aaaaatgatt ttcactcaaa
      241 gctggaaatg gtttgcagga aaggttatct ctaactccat tttcaaaaag tgattttgct
      301 ctgaatgcaa tcatcgcagc attatcacca cagaattgaa gatcaggaat aacaatattt
      361 tttttaaact tttctgcaag tttagttatt tctgatttca ggtgagcgtt tgccgcaact
      421 ccaccaacaa cagaaattga ttttacatca aaattttcta aagctcgttt aacgtttctt
      481 gttaaagctt taaccgcagc aacctgaaac gatgcggcaa tatcattttt atcctgtaaa
      541 gataatttgt caacccctcc ttgcgattgg acatgccgaa gtacagcagt ttttaatccg
      601 ctaaaagaaa aatttaattt ttgttttaaa tcagaaacag ggaaatcaat tttgcttcca
      661 tcaccaagtt tagcagcatt ttgtattttt ggtccaccgg gataacccag acccaataac
      721 tttgaaactt tatcaaaagc ttcacccgca gcatcatcaa ctgtactacc gagtttataa
      781 atttctgtaa aactattaac aagaagcaaa agtgtgtgcc cgcccgaaac aaccaggctt
      841 aaaaacggaa actcaggttt ttgcggcatt aaaaatccgg aaaagatatg tccttcaatg
      901 tggttaactc caagaaatgg tttgtttaat ccatacgcta aaccttttgc ataagtcagc
      961 ccaacaagta aagcaccaat taatccagga ccagcagtag cggcaataag atcaacatca
     1021 cttaatgcaa tgttagattt ttttaaagca tctttcagta aaggattaac tatctgcagg
     1081 tgtgcacgac tagataattc ggggacgaca ccgccataat ttgtatggaa atcttgagaa
     1141 gcaattaagt ttgtggttaa ttcattatcc tttataattg caacagaagt ttcatcgcaa
     1201 gagctttcaa tacctaaaac aatcatcttc ttccaaaagt taaaaagctt ttagcaattg
     1261 ttcctgcaac ggaaggattt tcttttaatc gtctgacaga ataagcatac cagtctttgc
     1321 caaacggtac atatattctg attttgtaac catctgaatt tatcttatca cgcaagtctt
     1381 ctctaacacc taaaagcatt tgaaattcaa atttatcttt agaaatgttt tgctctgtta
     1441 tgcgagtata agcggcatca attaaaggtt tatcgtgtgt agcaatgcca acataattac
     1501 catctttaaa catttgatca agaattttta aataattatc gcggacttct tgtttacctt
     1561 tgtatgcgat tgcagctggc tcaacataaa tgcccttgca taatctgtag tttgtcccaa
     1621 ttttatttaa ttctactaca tcatcaaatg atctgcgcat ataagcttgg ataactacac
     1681 caacattttt ataatcggca taaattttct tgtaaagatt tattgtctta tctgtaaatg
     1741 gcgagtcttc catatcaata cgcacaaaat ttttatactc agcagctttc tttaccagct
     1801 caagtatttg ttcataagca aactgctcat caattgccag acccatttgt gttggtttaa
     1861 tagaaagatt ggccatcaat ttattttttt caatggcttc taaaacttct aatgcttcat
     1921 tcttacattt tattgattct gatttattcg caacagattc accaagtaca tccatcgttg
     1981 cataaatacc tttggagttt aattttttta caaagtcaac tgcatcctga agtgtttcac
     2041 ccgctatata ttttttagaa aagaatccaa caactggttt tggcattaat tgaacaaaac
     2101 gaacgataag attattgaaa gcgtccacaa caaactcctt taaaattgct ttccaaattt
     2161 agtgtatttt attgatggaa taaaaattct tttagcattg tagttattgt gagcgggttt
     2221 tcagggtaag taatcttaaa ttatttcaaa caaagcagga gaattgttta tcggctaaag
     2281 cgggattagc aatgataaaa acactattaa attcccaatg ttaataagag aagattaaac
     2341 ttcattttta gatcatcgaa actggtttcg ttagaaaaaa taaaatctgc acgtttcttt
     2401 ttttcttctt ccttaatttg atttgcttct ctcttacaaa aatcatcttc agttattcct
     2461 gatgcgatct ttcttgctaa tctcaaatcc ctttgcgcag taatcagaac cacaaaatca
     2521 aacatatcct ccatatcagc ttcataaatt aaagctgctt caacaaaaac aactttatct
     2581 gttttttcta tggtattaat tgattgctca attgaattaa ttactctggg gtgcaaaatg
     2641 gattctataa tagataattt tataggatca gaaaaaactt ccttagcaag atatcttaca
     2701 tcaggtttac catctttaaa tgattgttta ccgaattcct taataattaa ttctcgaact
     2761 actttatcat tacttaaaat gtctttagaa atttcatctg ctcttagtat tgaatatttt
     2821 gcttcttcag caaattttgt gaatgtactt ttacctgaac cgatgttacc tgttattcct
     2881 attttcataa taattctcat atttaatcta ttttgaacag aattaacaat aaggatccat
     2941 ttaaaataaa aagtcacact tcgacaagct cagtgtgaca tttattaaaa gaacaaaatt
     3001 gactaatact tattttgcgt aaccgatttt tctaacttct cgaattacaa caacttttat
     3061 ctgacctggg taatccattt cctcttcaat cttttgtgca atttctcttg ctaatctatc
     3121 tgcacttgca tcatcaactt tatcgtgttc aacaacaact ctcacttccc tgcctgcctg
     3181 tattgcataa gtttttgcaa cgccttcaaa agattttgcc agaagttcaa gattctctaa
     3241 tcgcttaaca taactttcga gaggttcgcg tcttgcgcca ggccttgcgc cgctaatagc
     3301 atcagcagac tgcactaatg ccgcaattgg atgttccatc tcaatatctt cgtgatgcga
     3361 tccaacagca ttgataacta tagcatgttc tttatatttt ttcgttagat cataaccaag
     3421 taatgcgtgc ggaccttcga tacttttatc aacagtctta ccaacatcgt gaagtaatcc
     3481 ggctctttta gccataacag gatcgagtcc taattcagca gccataattc ctgttaagta
     3541 tgcaacttca acactatgtt gtaaaagatt ttgaccatag cttgatcgat acttcatttt
     3601 accgatgtgt ttaactaatt ctccatgcat attgtgtaga ccaagctgca gaattgtatt
     3661 ttcgccttca cgctggattt cttcttcaag ctctgctgat actttttcca cgacctcttc
     3721 aattcgtgca ggatgaattc ttccatcaga gattaatctt tccaaagaaa ttcttgcaat
     3781 ttctcttctg aattgatcga aagaagaaag gatgactgct tcaggtgtat catctacaat
     3841 aacatctaca ccagtagctg attcaaaagc acggatgttt cgaccttcct taccaattat
     3901 tctacccttc atttcatcat tttgaatctg aacaactgaa acagtagttt caatagagta
     3961 atcagctgca gttctttgaa tggcctgaac aattattttc tgtgcttctt ttttagcatc
     4021 aacttttgcg cgatcgtgta tttcttttat tgtctgtgag gcatctatct tagcctggtt
     4081 aatcatattt tcagcgagca ttttctttgc ttcttcggaa gtgaggccag aaattttttc
     4141 aagtttatca ttctgctcaa cttcaagctt tttgatctca actgattttt catccaagct
     4201 tactttcaaa tcctgaagtt gtttttccag ttttcttaat tctttttctt tttgcagaac
     4261 tagatcaaat ttcttttcgc tgttttcttc ccttgtttga atttgttttt cgagattaga
     4321 gagcttttgt tttttctgat ttatctcagt atcaaactca atttttttct tgtaccattc
     4381 atcttttact tcaagaagtt tttcgcgttt tagattagct gcttctttct ttgcatcctg
     4441 gatcagttgt tcggcttttt cttcggcagc aacgatgctt ttcttcccaa cttttgaatt
     4501 aaacatccag cctaaataaa agaaaagaac aatcagaacc acgatcatgg gtataattat
     4561 aatcaaatcc atcttttttc tccgtactat atttggaatt aaaaaccgac actgccgcgg
     4621 ctaataggaa aaaccctata aagcacggtg ggaacgcgct cgaaagcttt ttacttccac
     4681 tgcttcaaca aagttaaaga ctcaaacaag ttgagttgag gcctgtaata cgcttcgaag
     4741 gtcacactcc ctaattaatt caactgttag tttttcatta aaaaacggca gtgcggtaaa
     4801 ttttaagatg ggtcagacat ttcaggttct ttaagcagaa gctttattct ctttatttta
     4861 tcggtagctt gtatcagaaa ctcacggttt ttattttttt caataaataa atcgtaggca
     4921 atatttaaac aagcaataat agcaatagtt tgagcaggct gatccggcaa ttcgtctttt
     4981 gtttcttcca ttactttatt tacataaata gccagttctt ttgctatctc ttcattttct
     5041 actaatagag agtattcttt atcaaagatt tttattttta gcttcttttt atcagtcatt
     5101 gaattatcta caccatcaaa caatcatttt attaataaga actattttta tgaacttaag
     5161 tgaaaatcaa ttttagaaat aatatcctgt aactttagtt ttaaattttc cttctccttg
     5221 gaatttaatg tattaaatat atttccctct tcatcttgtt ttgataacaa aatttcctgc
     5281 tccaattcaa taaccttttt ttgcaataat gaattatcct gtcgcaattt tgcaacttga
     5341 atttctaatt ccgtttttct ttcttcagta tctcttagct tattgccgag ggttgcaatt
     5401 tctgcttcga ttgctgaaag atcgtgaatt attgaatcat attttgctaa atcaaccaat
     5461 tattttcctc ttaattgagc gttaaactct ttggttataa gtgcaattaa attgttaaaa
     5521 tctttttcaa cttcatcttc tgtaagagtt ctgttatcat cataatattc taaagtaaat
     5581 gctatacttt ttctatcttt acctaacgaa tcgctttcaa acacatcaaa tacagaaatt
     5641 actttaagaa gttgtgaact cttctttctg ataaattctt ttacatcaag atatttaatt
     5701 gatttatcca aaatgaacgc aaaatctctt attattttgg gatattttaa tggatcagta
     5761 taagtttttg aaatcgattc aattgatttt aaagccgata aatcaaactc aaaacagtaa
     5821 acatcctgat tgatatcaaa ttgtttaaga acctcttgcc ttaacttacc gcctcttcct
     5881 aactctactt ctttaaagct ttttgttaag taataggcgt aaatttcatt tgcagtcgaa
     5941 taatatgaat caattaaaac attgtcaagt gatattttac ttaaaaatga atcaaccaaa
     6001 ccctttagaa gatagaaatc tgattccttt tcgtctgtat tccatccttt tatttgatct
     6061 ttaccgctaa ttgcaaatag taaattttga gtttcactaa aatctgaaaa ggtttttatc
     6121 tccccttcag atatttttat gaatgaatcc ccgatctcaa atagttttag ttctctgaca
     6181 ccctgtctga tatttttaga tattgtaatc aaagctccgg ataataaaga agttcttaaa
     6241 cattccatat cagcgcttaa ggggtttgca attttaattg gactcccggt taaccgggca
     6301 tcattttcag attgcaaagg attgtttatc atttcaaaaa agccaagtgc ggtagaagct
     6361 tgtcttatct tttcatcaat ttcagtttca tctgttttct tttcaagcgt aatattaatt
     6421 tttgaaacgg ttggaatatt atcataacca ttaattctgg caatttcttc aattagatca
     6481 atttctcgtt caatatcggg acggaatgtc gggattgaaa caagcagttt atcctgatct
     6541 aaaactttaa ctgctaagcc caatcttgat aaaatatttt taattttttc agatggaact
     6601 tcataaccta aaattcttgt tgtgcgtgag aaccttaatt caatttcctt tggcaaaatt
     6661 acattcggat aaacatcaat tattcccttt gcaacttttc catcagctaa ttcagcaaca
     6721 agctgcgcgc atctttcagc agcatatttg gtaatatttg gatcagttcc tctttcaaat
     6781 cgataagaag catctgtgct taatcctaaa gttttagaag tttttctgat gcttgaagga
     6841 ttgaaataag cactttctat taaaatgttg tttgtggtat cagtaatttc agaattttct
     6901 ccacccataa cacctgcaat cgcaacttct ctgtttccat cgcaaatcat caaagtattt
     6961 acaggaagtt ttcgttcttt agaatcaaga gttgtaaaa
//
LOCUS       DASWBY010000704         4427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_481610, whole genome shotgun sequence.
ACCESSION   DASWBY010000704 DASWBY010000000
VERSION     DASWBY010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481610"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="VF870_12860"
     CDS             <1..1407
                     /locus_tag="VF870_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX9253129.1"
                     /translation="CSTSEMSAKGESGMKINLDKVPLREKGMTAYEIMLSESQERMLC
                     CVKKGYEDRVKQVFEKWDLHCEIIGEVTGDGLLHIDYQGERKATIPPFDLVLGGGAPV
                     YTREQKEPGYLKETRKFDSKTLPEPKDLKETFLKVFSSPNIVSKQWVYHQYDSMVRTN
                     TVVGPGCDAAVILVKGTNKALAMKTDCNSRYVYLNPKEGTKIAVAESARNIVCSGGVP
                     LGVTNCLNFGNPYKPEVYWQFAQAIAGMGEACRHFDTPVTGGNVSFYNESPDTAVYPT
                     PTIGMVGLVEDLKHITTSYFKDEGDVIYLLGEDKEELGGSEYAKVIHNKVAGESPSIN
                     LDEEKKLQETLLNLIRKGLIKSAHDISEGGIASALAECSIINQEKQIGCEVEIPIKSR
                     KDFSLFSESQSRIIISVSEDNIDELEPELEKSELKFSKLGIVTGNDLKLKNLFEVSVN
                     NLSDIYYNTIPGIMSGEE"
     gene            1397..2389
                     /locus_tag="VF870_12865"
     CDS             1397..2389
                     /locus_tag="VF870_12865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX9253130.1"
                     /translation="MKNKLKQVYKNSSLFKYFRQIRYLYHTRPHWQKIGSFLKHYFGG
                     LYLRFDTHHIFLFSGGLAFSLFTCIIPLILIVFWILGNFLSSEEMELQIITFINTVIP
                     YSEYADFVKQIIFSRVEEVIELKNVAGVLGFVGLFFAASGFFSSMRTVLNKINGKDID
                     VNIFIGKLRDFLVILVAILLFFASILALPLLELFKSIAESTPYLQFFNHPIFQQLFTI
                     LVSLFIMLVLMYLFYSFIPTAKIKHRSALVGAAWASLFWVGMKVLFGYYLANFQTWGR
                     IYGAYALVIVIAFWIYYTAAVFIIGAEIAKLFDERLKEKIAAKHARPENLPPLK"
     gene            2434..3123
                     /locus_tag="VF870_12870"
     CDS             2434..3123
                     /locus_tag="VF870_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9253131.1"
                     /translation="MAELKTNQTLRDIPLFSELSIEHLRKISSFSKIKSFNKHDRIFN
                     EDDYYMGFYILLKGTVKVYKISSKGKESVVHIVKPLTAFADIPLFEGGNYPVSAEAIE
                     ESLTLFIPKEKFLELIYKEPEISLKMLAGFAKRLKSLINQLEDLSSKEVPNRLAKYIL
                     KEIKITGTEKLPEPFVKLSIPKSTIASYIGTITETLSRSFKKLQDEGIIRVNGKKIFI
                     NDMKRLKELAR"
     gene            3263..3502
                     /locus_tag="VF870_12875"
     CDS             3263..3502
                     /locus_tag="VF870_12875"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX9253132.1"
                     /translation="MEIKTLDIRPVPPREKHPTIFKTFDELKDGEGFQLVNDHDPKPL
                     YYQFNAERSNQFKWAFVEQGPEVWRVNIFKISGLK"
     gene            3503..3958
                     /locus_tag="VF870_12880"
     CDS             3503..3958
                     /locus_tag="VF870_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9253133.1"
                     /translation="MFTTVRYFVKTSLIFLIVGILTGLHMSFTKYISQKSYTQELISA
                     HTHIILVGSVMMMIMGVALWFFPRAEKDDKKYNPDLILITYWIMTIATAMRFVMQIIG
                     AYYYLPWVNVSIVVFSSFQVLAMILFFYSMWGRIRAVGSQYREAKGEKF"
     gene            3958..4206
                     /locus_tag="VF870_12885"
     CDS             3958..4206
                     /locus_tag="VF870_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9253134.1"
                     /translation="MPIVITEDCINCGACAVECPTNAICKPAESKIVNGKYYSPISTE
                     HFFILDKLCNNCTGFEVIKCIEICPMNAFQANTNFSNY"
BASE COUNT         1513 a          672 c          820 g         1422 t
ORIGIN      
        1 tgttcaacaa gtgagatgag tgcaaaaggc gaatcaggaa tgaagatcaa tcttgataaa
       61 gttcctttgc gcgaaaaggg tatgactgct tatgaaatta tgttgagcga aagccaggaa
      121 agaatgttgt gctgtgttaa aaaaggatat gaagatcgtg taaagcaagt ttttgaaaaa
      181 tgggatttac attgtgaaat tatcggcgaa gttacaggcg atggtttact tcatattgat
      241 tatcaaggag aacgaaaagc aacaatacct ccttttgatt tggtacttgg cggtggcgct
      301 ccggtttata ctcgtgagca aaaagaacca ggatacttaa aagaaacaag aaaatttgat
      361 tcaaaaacat taccagaacc aaaagacctt aaagaaacat ttctaaaagt attttcatct
      421 ccaaatattg tttcaaagca atgggtttat catcaatacg attctatggt aagaacaaat
      481 acagttgttg gtcccggttg cgatgctgca gttattttag ttaagggaac taacaaagcg
      541 ttagcgatga aaactgattg caactctcgt tatgtttatc tcaatccaaa agaaggaaca
      601 aagattgcag ttgcagaatc tgcaagaaat attgtttgtt caggcggtgt tccgcttgga
      661 gttacaaatt gtttgaactt tgggaatcct tacaagccgg aagtttactg gcagtttgct
      721 caagccatag caggaatggg agaagcatgc agacattttg atactccggt tacaggcggt
      781 aatgtaagtt tttataatga gtcaccggac acagcagttt acccaacgcc gactatagga
      841 atggttggat tggttgaaga tttaaaacac attacaacat cttactttaa agatgaaggt
      901 gatgtaattt atctattagg tgaagacaaa gaagaactcg gcggaagcga atatgctaag
      961 gttattcata ataaagttgc aggagaatct ccatcaataa atcttgatga agaaaaaaaa
     1021 ttgcaggaaa cattattaaa tcttatcaga aagggattga ttaaatctgc tcacgatatt
     1081 tctgaaggcg gaattgcttc tgcattagca gaatgcagta tcatcaatca ggaaaaacaa
     1141 attggatgcg aagttgaaat tccgattaaa tccagaaaag atttttcttt attctcagaa
     1201 tcacaatcga gaataatcat ttctgtttcc gaagataata ttgatgaact tgaacctgaa
     1261 cttgaaaaat cagaactaaa atttagtaag ctaggtatcg ttacaggaaa tgatttaaaa
     1321 ttaaaaaatc tttttgaagt aagcgtgaat aacctttctg atatttatta taacacaatt
     1381 cctggaataa tgtccggtga agaataaact taaacaagtt tataaaaatt cttctttgtt
     1441 taaatatttt agacaaataa gatatttgta tcatactcgg cctcattggc aaaaaattgg
     1501 atcgtttctt aagcactatt ttggcgggct ctatctccgg ttcgatactc atcatatatt
     1561 tttgttttcc ggaggactgg ccttttcact ttttacatgc atcatcccat taattctaat
     1621 tgtgttctgg atactcggaa attttttaag ttcagaagag atggaattgc aaataataac
     1681 gttcataaat actgtaattc cttatagcga gtatgcagat tttgtaaagc agattatatt
     1741 tagtcgtgtt gaagaagtta tagaattaaa aaacgttgcc ggagttttag gttttgtagg
     1801 tttatttttt gcagcaagcg gatttttcag cagtatgcgt actgtattaa ataaaataaa
     1861 tggcaaagac atcgatgtaa acatttttat tggcaaacta agagattttc ttgtgattct
     1921 tgtggcaata ctccttttct ttgcatcaat tttagcactt cctcttttgg agttatttaa
     1981 atctatcgcc gagtcaactc cttatcttca gttttttaat catccgattt ttcagcaatt
     2041 gtttacaatt ctggtttcac tttttataat gctggttctt atgtatctgt tttacagttt
     2101 tattccgact gctaaaataa agcaccgttc tgccttggtt ggagcagcct gggcatcatt
     2161 gttttgggta ggaatgaaag ttttatttgg atattatctt gcaaactttc aaacctgggg
     2221 aagaatttat ggtgcttatg ccctggttat tgttattgcc ttctggatct attatacggc
     2281 agcagttttt attataggtg cagaaatagc aaaactattt gacgaacgat taaaagaaaa
     2341 gattgctgca aaacacgcta ggccagaaaa tctacctcca ctaaagtaga gttaataata
     2401 catcgtttta ataattttca atttgcattg gatatggcag aattaaaaac taatcaaaca
     2461 cttcgtgaca ttcctctttt ttcagaacta agcatagagc atcttcgcaa aatctcttca
     2521 ttcagtaaaa ttaaaagttt taacaaacat gatagaatat ttaatgaaga tgattattac
     2581 atgggatttt atattctatt aaaaggaaca gtaaaagtct acaaaatatc ttccaaagga
     2641 aaggaatcgg ttgttcatat tgtaaaacca ctaactgcat ttgcagatat tccgttgttt
     2701 gaaggtggaa attatccggt tagcgcagaa gcgattgaag aaagtttgac tctcttcatc
     2761 ccaaaagaaa aatttctgga acttatttac aaagaaccag aaatttcttt aaagatgctt
     2821 gcaggatttg ctaaaagatt aaaatcactt ataaatcagc ttgaagatct ttcttcaaaa
     2881 gaagttccaa atcgattagc gaaatatatt ttgaaggaaa taaaaattac cggtacggaa
     2941 aaacttccgg agccatttgt gaaactttcg attcccaaat caacaattgc atcttatatt
     3001 ggtacgataa cagaaactct ttcaaggagt ttcaaaaaac ttcaggatga aggaataatc
     3061 cgcgtaaatg gaaaaaagat ttttattaat gatatgaaac gattaaaaga actggccagg
     3121 taaacctatt ttttgaaact cattttgctc gacacagata ttaataaatc ctaaaacaca
     3181 cataatctga cttaagtcaa ggaaatcctt tgtcaagttt cataccttta aattgagaat
     3241 tacacatagc aaaggagtta caatggaaat aaaaacactt gacattcggc ctgtaccacc
     3301 acgcgaaaag catcccacta tctttaaaac ttttgatgaa cttaaagatg gcgaaggatt
     3361 tcaattagta aatgatcacg accctaagcc actgtattat caatttaatg ctgaaagatc
     3421 aaatcagttt aaatgggcat ttgttgagca gggtccggag gtatggcgtg taaatatttt
     3481 caaaatatca ggattaaaat aaatgtttac aaccgttaga tattttgtta aaaccagttt
     3541 aatttttcta attgtaggaa tattgaccgg tttacatatg tcatttacta aatacatctc
     3601 acaaaaaagc tatacccagg aattaatttc agcacatact cacatcatcc ttgtgggttc
     3661 agttatgatg atgattatgg gtgtggcact ctggtttttt ccgcgtgcag aaaaggatga
     3721 taaaaaatat aaccccgatt taattttaat tacttattgg ataatgacga tagcaacagc
     3781 gatgcgattt gttatgcaga taattggtgc atattactat cttccatggg ttaatgtatc
     3841 tattgtggtt ttctcgtctt ttcaagtgct ggcaatgatt ttatttttct attcaatgtg
     3901 gggaagaatc agagctgttg gaagtcagta tcgggaagca aagggagaga agttttaatg
     3961 ccaatagtaa ttacagaaga ttgtatcaac tgtggagcct gtgcagttga atgtccaaca
     4021 aacgcaattt gtaagcccgc tgaaagcaaa attgtaaatg gaaagtatta ttcgccaatt
     4081 tcaacagaac atttttttat tttagataag ctttgcaata actgcacagg atttgaagtg
     4141 attaaatgta ttgaaatctg tccgatgaat gcatttcaag caaatacaaa cttctccaac
     4201 tattaaagtc ttttaaattg aaatattgat ttttatatta ataatagcac gatagtatcg
     4261 ataatcaatt tgaacagagg taaaatatga aagagttcag ggtaaaagtt ctgcgaatag
     4321 ataatgttac acatgatgta ttgtgtatta aagttcaaaa acctgatggg tttaaattta
     4381 caccagggca ggcaactgat gttgccatta ataagcctgg ctatgtt
//
LOCUS       DASWBY010000705         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_554312, whole genome shotgun sequence.
ACCESSION   DASWBY010000705 DASWBY010000000
VERSION     DASWBY010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554312"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VF870_12890"
     CDS             <1..714
                     /locus_tag="VF870_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253135.1"
                     /translation="FNSLEGWYSYYRFASANNVEYQVFKKDTAVTVNSVAATIRFELI
                     GKKLADETINTEIGNFLCKKFVMEVRISYLLLNIFPVKLFGVEDSLWFAPDNWKVKSY
                     IPATNVDLSYLNIPAFYIPGLETNIITPITNLETEINTANNFELYQNYPNPFNPATRI
                     QYTISNRQFVQLKVFDVLGNEVATLVDEEKPAGNYEVDFTVSKSADGGSRPAIASCVY
                     YYQLKVGDLTQTKKMILIK"
     gene            779..2626
                     /gene="pepF"
                     /locus_tag="VF870_12895"
     CDS             779..2626
                     /gene="pepF"
                     /locus_tag="VF870_12895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX9253136.1"
                     /translation="MFSHDLISKLFSTEAKTIEVPSRDEIDEKYKWNLKDIYSSDAEW
                     EKEFKFVEQSIFGYAQYEGRITDSAENLLACLKFDEEIGIKLERLYLYSMLAKDSDMR
                     DPKYQGMDDRIKSVYSKVLTASSFIKPELLKADEEKLLGFIDSNSELKIYKQSFDDLI
                     RFRKHTLEREQEELLAMASEVTQVPYNTYSLFTNADLKFPKVADEKGNEIEMSHSRFY
                     SALYSKDRDYRRRAFKAYLKPYVDNVNSFSSLFNGNLKSNIFYAKARKYSSAREAALF
                     KNNIPISVYDNLVEAVNKNISPMYRWASLKKKLLNLDELHPYDVYVTLFDQKLEKKYS
                     YEESKQIVLDSLKIMGEDYLTSLNNAFDNRWIDVYETKAKRSGAYSSGTTFGVHPYVL
                     LNWTDLLNDVFTLAHEMGHNMHSYYTGLHQPYTYANYSIFLAEVASTFNENLLLDHLI
                     KISESKEEKLFLLEKYLNNITTTVYRQVMFAEFESLTHFKAESGEALTPDVLCKLYKD
                     VYKKYWGPEMVIDNEESYTWARVPHFYYNFYVYQYATGFAASEALALKVKSEGGPAVQ
                     KYLDFLKAGSSDYPINILKKAGVDMNSQEPILAVTKKMNQIIDEIENLI"
     gene            2645..>3482
                     /locus_tag="VF870_12900"
     CDS             2645..>3482
                     /locus_tag="VF870_12900"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9253137.1"
                     /translation="MKVYTKIELNAFNQSLTYDDISLIPTQVSRIKSRTEASTNCSFL
                     GIKLNVPVISSPMDSVTGTEMAKALSNLGSLGIVNRFDSSLDSVLNSKNGRGIKAVSI
                     ALNTDLKTIEKIAEKNYLICIDTANANNKEVLKKTEMIKKKFDVKVIVGNIAHGESLD
                     QIENSGADAVRVGIGSGSVCTTSIQTGIGIGQVSSLLNILFSRKQKKLKIKIIADGGV
                     KSPGDVAKAIALGADVVMLGRMLSGTRETPGEVIKYNGQLWKKYRGSASFGVKMRNEF
                     IEG"
BASE COUNT         1240 a          523 c          641 g         1078 t
ORIGIN      
        1 ttcaattcac tcgagggctg gtattcttac tatcgatttg catctgcgaa taatgttgaa
       61 tatcaggtat ttaaaaaaga cactgctgta actgtaaatt ctgttgcggc aactattaga
      121 tttgaactaa taggaaaaaa gctggctgat gagacaatta atacagaaat aggtaatttt
      181 ctctgcaaaa aatttgtaat ggaagtaaga ataagctatt tattgttgaa catttttccg
      241 gtcaaacttt ttggtgtgga agattctctt tggtttgcac ctgataattg gaaagtaaaa
      301 agttatatac cagctacgaa tgtggattta tcctatttga atataccagc tttttatatc
      361 ccaggcttgg agacaaacat tataactcca attacaaatt tggaaactga aattaatacg
      421 gctaataatt ttgaactcta tcaaaattat ccaaatccat ttaatcctgc taccagaata
      481 caatatacta taagtaacag gcaatttgta cagttaaaag tttttgatgt attggggaat
      541 gaggtagcaa cattagttga tgaagaaaaa cctgccggaa attatgaagt tgattttacg
      601 gtttcaaaat cagccgatgg cggatcccgt cctgcaattg caagctgtgt ttattattat
      661 caactaaaag ttggtgatct aacccaaaca aaaaaaatga tactaatcaa atgattattt
      721 tatcttttat tcaattaaaa aatataatta acttttattc cactatctgg ggatacagat
      781 gttcagccac gatctaatat caaaattatt tagcactgaa gctaaaacaa tcgaagttcc
      841 ctcccgagac gaaattgacg aaaagtataa atggaatctg aaagatattt atagttcgga
      901 tgcggaatgg gaaaaggaat ttaaatttgt tgaacaaagt atttttggct atgcacaata
      961 tgaaggaagg ataaccgatt cggcagaaaa tcttttagct tgtcttaaat ttgatgaaga
     1021 gataggaatc aaattagaaa gattgtatct ctattctatg cttgccaaag acagcgatat
     1081 gagagatcca aaataccagg gaatggacga tagaattaaa agcgtctatt ccaaagttct
     1141 tactgccagc tcttttatta agcctgaact gttaaaagcg gatgaagaaa aattactggg
     1201 atttatcgac tcgaattccg agctcaaaat ttataagcaa tcttttgatg atcttataag
     1261 gtttagaaaa cacacactcg agcgtgaaca ggaagagttg cttgcaatgg caagtgaagt
     1321 tactcaggtt ccttataata cctactcact ttttacaaac gctgatttaa agtttccaaa
     1381 agtagctgat gaaaagggga atgaaattga aatgtcgcat tcccgatttt attctgctct
     1441 gtattctaag gatagagatt atcgcagacg ggcatttaaa gcatatttaa aaccttatgt
     1501 ggataatgtt aattcattca gttcattgtt taatggaaat ttgaaatcaa atatttttta
     1561 tgctaaagca agaaagtaca gctcagcaag agaagccgca ttatttaaaa ataatattcc
     1621 aatttcagtg tacgataatc ttgttgaagc tgtcaacaaa aatatttcac caatgtatcg
     1681 atgggcatca ttaaagaaaa aacttcttaa tcttgatgaa cttcatccct atgatgttta
     1741 tgtaactttg tttgatcaga agcttgagaa aaaatattct tatgaagaat caaaacaaat
     1801 tgttttagat tcattaaaaa ttatgggtga agattatcta acatcgctta ataatgcttt
     1861 tgataacaga tggattgatg tttacgaaac taaagctaaa agaagcgggg catattcttc
     1921 gggcaccaca ttcggggtgc atccttatgt tttattaaac tggactgatt tactaaatga
     1981 tgtttttact ttagcgcacg aaatgggtca taatatgcac tcgtattata caggattgca
     2041 tcaaccgtat acatacgcaa attattcaat ctttcttgct gaagttgcat ctacatttaa
     2101 tgaaaatctt ttgcttgatc atctcattaa aatttctgaa tcaaaagaag aaaaactttt
     2161 cttgttagaa aagtacctaa ataacattac aactacagtt tacaggcagg taatgtttgc
     2221 tgaatttgaa tcgctgacac attttaaagc tgaaagcggg gaagcactta ctcctgatgt
     2281 tttgtgtaaa ctttataaag atgtttacaa aaaatattgg ggtcccgaaa tggttattga
     2341 taatgaagaa agctatacct gggctcgcgt acctcacttt tattacaact tttatgttta
     2401 tcaatatgca accggatttg ctgcctctga agcgcttgct ttaaaagtta aatccgaagg
     2461 tggtcctgct gtacaaaagt atttggattt tctaaaggct ggtagttccg actatccgat
     2521 taatattctt aaaaaagctg gagtcgatat gaactcacag gaaccaatat tagcagttac
     2581 aaaaaaaatg aatcaaataa ttgatgaaat agaaaactta atctgaacta aatacaggat
     2641 ataaatgaaa gtatacacta agatagaact aaatgcgttc aatcaatcac taacatatga
     2701 tgatataagt ctgataccaa cacaggtttc cagaataaaa tcacgaacag aagcatctac
     2761 aaattgttct tttctcggaa taaagcttaa cgtaccggtt atttcaagtc caatggattc
     2821 tgtaacagga actgaaatgg caaaagctct ttcaaatctc gggagtcttg gaattgtaaa
     2881 tcgatttgat tcttctcttg attcagtttt aaattctaaa aatggaagag ggataaaagc
     2941 agtatctata gcgctaaata ctgatctgaa gacaattgag aaaattgcag aaaaaaatta
     3001 tctgatttgt attgatactg ctaatgctaa caacaaagaa gttttgaaaa aaactgaaat
     3061 gattaaaaag aaatttgatg ttaaagttat tgttggtaac atagctcacg gtgaaagtct
     3121 tgatcaaatt gaaaattcag gagccgatgc tgttcgtgtt ggaataggga gcggaagtgt
     3181 ttgtacaacc tctatacaaa ccggaatagg aatagggcag gtatcatccc tgttaaatat
     3241 tttattttcc agaaaacaga aaaaactaaa aataaaaatc attgcagatg gcggagttaa
     3301 aagtcccggc gatgttgcaa aggcaattgc actgggagct gatgttgtaa tgttgggaag
     3361 aatgctatca ggaactcgtg aaactccggg tgaagtcata aaatataatg gacagctctg
     3421 gaaaaaatat cgcggctctg cttcgtttgg agtaaaaatg cgaaatgaat ttattgaggg
     3481 tg
//
LOCUS       DASWBY010000706         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_30708, whole genome shotgun sequence.
ACCESSION   DASWBY010000706 DASWBY010000000
VERSION     DASWBY010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30708"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VF870_12905"
     CDS             <1..634
                     /locus_tag="VF870_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HEX9253138.1"
                     /translation="LILNSAFSGHAVVGGFLGAGMKEAIRFGIARGLFSNESGLGSAP
                     IVAAAAQTKNPVRQALVSSTGTFWDTVVVCAMTGLVIVNSGEWVKGLSGAALTKQAFS
                     DFHIVGPIVLTLGLLTFVFSTILGWSYYGEKAAEYLFGNKVVKPYRYLWVMFVMIGSV
                     MSLNVVWTFADITNALMAVPNLVSLILLSGIIVKETKKYLWEDKLDEEGE"
     gene            complement(642..1886)
                     /gene="serA"
                     /locus_tag="VF870_12910"
     CDS             complement(642..1886)
                     /gene="serA"
                     /locus_tag="VF870_12910"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX9253139.1"
                     /translation="MSNTKKSLSLSKDKIKILLLEGLHNNALQLFNNNQYTNIEYIKS
                     SLETAELIEKIKDVHLLGIRSKTNITKDIFKHANKLIAIGCYSIGTNQVDLLSAKRQG
                     IPVFNAPFSNTRSVAELVIAECIFLIRGIPEKNFAAHNGEWLKDASNSYEVRGKNLGI
                     VGYGHIGSQVSILAESLGMNVYYYDIEKKLNLGNAKSCSSLRELLSISDIVTLHVPET
                     ELTKNMIGANELSLMKKGSYLLNASRGTVVVIDDLAKALEENHLAGAAIDVFPEEPNS
                     NKDSFKNVLQKFPNVILTPHIGGSTSEAQANIALEVSEKLVKYCDVGTTIGATNFVEV
                     ALTPNLNKQRYLHIHKNQPGVLNKITKVFTSRKLNIASQFLQTDAHIGYVIIDIDQKN
                     NTEEIMKELKAIPETIKTRMLL"
     gene            complement(2004..2267)
                     /locus_tag="VF870_12915"
     CDS             complement(2004..2267)
                     /locus_tag="VF870_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253140.1"
                     /translation="MDLRRRAFGLALGLMLGLSFLLGNFWLLIIDSQGDFFSKFSSFF
                     FGYTFTWLGALVGFFWAFVYGFFFGVFLAWLYNIISGIIYSNR"
     gene            complement(2283..>2612)
                     /locus_tag="VF870_12920"
     CDS             complement(2283..>2612)
                     /locus_tag="VF870_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253141.1"
                     /translation="EYHQFFSLFDGSIIPNNWLPILFVIAAGLTGYIYSFGFFKLSKF
                     AAHFWVPYTNIWLALLPFIMLLFGKDVTIYQIIGAVVVTAGLMVGVMDYSKNKVVEIE
                     KKKHIQK"
BASE COUNT          852 a          448 c          458 g          854 t
ORIGIN      
        1 gcttattctt aatagtgcat ttagcggaca cgcagtagtt ggcgggtttc ttggcgcagg
       61 aatgaaagag gcgataagat ttggaattgc tcgcggactt ttttcaaatg aatcgggttt
      121 gggaagtgca ccgatagttg cagcagcggc acaaacaaaa aatccggtgc ggcaggcatt
      181 agtttcatca acaggaacat tttgggatac agttgttgta tgcgcaatga caggattggt
      241 aattgtaaat tcgggtgagt gggtgaaagg actttcagga gccgcattaa caaaacaagc
      301 gttcagtgat tttcacattg ttggtccaat tgtgcttaca cttggattat taacatttgt
      361 tttttctaca atactcggtt ggtcttatta cggtgaaaaa gctgcggaat atcttttcgg
      421 aaataaagtt gtaaaaccgt accgctatct ttgggttatg tttgttatga tcggttcggt
      481 tatgtcttta aatgttgttt ggacttttgc agatattaca aatgcactaa tggcagttcc
      541 aaaccttgtt tcactaatcc tattaagtgg gattattgtt aaagaaacaa aaaaatatct
      601 ttgggaagat aaacttgatg aagaaggaga ataaatagca actaaagcag cattcttgtt
      661 ttaatagttt ccggaattgc ttttagttct ttcattattt cttcggtatt atttttttga
      721 tcaatatcaa ttattacata accgatgtgc gcatcggtct gtaagaattg agatgcaatg
      781 ttaagttttc ttgaagtaaa tacttttgta attttgttta atactcccgg ctgattttta
      841 tggatgtgta aatatctttg cttgtttaaa tttggcgtta atgcaacttc aacaaaattt
      901 gtggctccga tagttgttcc gacatcacaa tatttaacta acttttcaga aacttccagc
      961 gcaatgttcg cctgtgcttc tgatgtgctg ccgccaatgt gcggagttaa aattacattt
     1021 ggaaattttt gtaaaacatt tttgaatgaa tctttgttag agtttggttc ttcaggaaat
     1081 acatcaatcg ctgcaccggc aagatgattt tcttccaaag cttttgctaa atcatcaatt
     1141 acaacgacag tccctcttga ggcgttaagc aaataagaac cttttttcat aaggcttaat
     1201 tcatttgcac caatcatgtt ttttgtaagt tctgtttccg gaacgtgaag tgttacaata
     1261 tcggaaattg aaagaagttc ccgcaaactt gaacaggatt ttgcgttacc aagatttaat
     1321 tttttctcga tatcatagta gtaaacattc attcccagtg attctgcaag aatagaaacc
     1381 tgtgaaccaa tgtgtccgta tccgacaatt cctaaattct ttcctctaac ttcgtaggag
     1441 ttagaagcat ctttaagcca ttcaccatta tgtgctgcaa agtttttttc cggaattcca
     1501 cgaataagaa aaatacattc agcaattaca agctctgcaa cagatcgcgt attagaaaac
     1561 ggtgcgttaa ataccggaat tccctgccgt tttgccgata ataaatcaac ctggttggtg
     1621 ccgatgctgt aacagccaat agcaataagt ttattggcgt gtttaaagat atcctttgta
     1681 atgtttgttt tggatcttat cccaagcaga tgtacatctt taattttttc tatcagctca
     1741 gcagtttcta aagaactttt gatgtactca atatttgtat attgattatt attaaaaagc
     1801 tgtaaagcat tgttatgcaa tccctcaaga agaagaattt ttattttgtc ctttgaaagc
     1861 gataaagatt tttttgtatt agacatatta ttttttttga attttatagc acaaacataa
     1921 agagcaatta taaaacgata aataaattat taatgaatta attaaaagtg tctttaaact
     1981 aaaatattat ttataaaaat ttatcatcta ttactataaa taataccgct aataatgtta
     2041 tacaaccacg caagaaaaac accgaaaaag aatccgtaaa caaatgccca gaaaaatccc
     2101 acaagagcgc caagccaggt aaaagtatag ccaaaaaaga atgacgaaaa tttagaaaaa
     2161 aaatctccct gcgaatcaat aattaataac caaaaatttc ctaagagaaa tgataagcca
     2221 agcattaaac ccaacgctaa tccaaatgct cttcttctca aatccataaa taccctttaa
     2281 aattatttct gaatgtgttt tttcttttcg atctctacaa ccttgttttt actgtaatcc
     2341 ataaccccga ccattaaccc tgcagttaca acaacagcac caatgatctg ataaattgta
     2401 acatcttttc cgaatagcag cataataaaa ggaagcaatg cgagccagat gttggtatat
     2461 ggtacccaaa aatgtgctgc aaattttgaa agcttgaaga aaccaaagct gtaaatatac
     2521 cccgttaagc cagcggcgat aacgaaaagt atcggcagcc agttatttgg aattatactt
     2581 ccatcaaaca aagaaaagaa ttgatgatac tc
//
LOCUS       DASWBY010000707         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_481680, whole genome shotgun sequence.
ACCESSION   DASWBY010000707 DASWBY010000000
VERSION     DASWBY010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481680"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VF870_12925"
     CDS             <1..1120
                     /locus_tag="VF870_12925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9253142.1"
                     /translation="EKLTKVIVAETEQNKPNQQNQTEKSGTGQSQNNYRKGGRRYYPK
                     KRYYKPQGAPGEQQAPPTRISFQKISIVVPLLNEEESILPLINEIRKAIKPLNKPYEV
                     IFVDDGSTDNSLKTIKDAAKTDNRLKFISFRKNYGKSAALQVGFKAATGDAIITMDAD
                     LQDDPHEIPNLLKKLDEGFDLCSGWKKERFDPFIKKSSSRFFNFVTRVISGIKIHDFN
                     CGLKAYRREVVQNLNVYGELHRYVPVLAKWQGFTVTEIPVKHHPRRYGKTKFGVSRFF
                     KGFIDLITVTFVGRYVKRPMHFFGFLGALSFFVGLIVNGYLTIEWISGRPLSNRPMLF
                     LGMLLIIVGVQFFSVGLLGEMLVHQNQSEREYVVKDRN"
     gene            1160..2281
                     /locus_tag="VF870_12930"
     CDS             1160..2281
                     /locus_tag="VF870_12930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9253143.1"
                     /translation="MKIVIVSPAYPLRGGIANFTAQLYQELSLEHKVIVFNFKRQYPK
                     LFFPGKTQLEVGENVDKIPSRIEIDSINPITWIKTANNIIAEKPDLVIFKYWMPFFAL
                     CYGVIAKKIKKRTKAKIIAVCHNIVPHEKKPGDDFLTKYFLSRMEYFILLSNQVENDL
                     KKVISDPKSKILPHPIYSRFGSAVDKQSALDYLKLKEYNYLLFFGFIRDYKGLDVLIE
                     AMNLIKDQDQIKLIVAGEFYEDEKKYLDLIAKYNLSNKIILIKDFIPTLDVKYYFSVC
                     DAVVLPYREATQSGIVQMAINFNKPVIAANVGGVSEVIVNDETGYIVEKENPKALADA
                     IKIFYTANKKELFNKNILHLKDKYSWKYFVSGLFDLINT"
BASE COUNT          836 a          338 c          408 g          723 t
ORIGIN      
        1 agagaaatta acaaaggtaa ttgtggcaga aacagagcaa aataaaccga atcaacaaaa
       61 tcaaaccgaa aaaagcggaa caggtcaatc tcaaaataat tatcgcaaag gcggaagaag
      121 atattatcct aaaaaaagat attataaacc gcaaggcgcg cctggagaac agcaggctcc
      181 accaaccaga attagtttcc aaaagatttc aattgtagtt ccgcttctaa atgaagaaga
      241 atcaattctt ccactcatta acgaaattag gaaagcaatt aaaccactta ataaacctta
      301 tgaagtaatc tttgttgatg atggaagtac tgataattct cttaaaacta ttaaagatgc
      361 tgcaaaaaca gataaccgat taaaatttat tagtttcaga aaaaattatg gtaaatccgc
      421 agcattgcag gttggattta aagctgcaac cggcgatgca atcattacaa tggatgccga
      481 tttacaggat gatccgcacg aaattccaaa tcttctaaaa aaacttgatg aaggttttga
      541 tctttgttcg ggttggaaga aagaaagatt tgatccgttt attaaaaagt cttcgtcacg
      601 ttttttcaat tttgtaacaa gagtaattag tggaattaaa attcacgatt ttaattgcgg
      661 attaaaggct tacagaagag aagttgtaca aaatttaaat gtgtatggag agcttcatcg
      721 ttacgttccc gttttagcaa agtggcaggg atttacggta acagaaattc cggttaaaca
      781 tcatccccgc aggtacggca aaacaaaatt cggtgtttcg agattcttca aaggtttcat
      841 cgatttaata accgttacat ttgttggcag atatgtaaaa cgcccaatgc acttttttgg
      901 atttttgggt gcattatcat tttttgttgg tttgatagtt aatggctact taacaattga
      961 gtggataagc ggaagaccat tgagcaaccg accgatgtta tttttaggta tgttgttgat
     1021 aattgtcggt gtacagttct tttctgttgg attgctcggt gaaatgcttg tacatcaaaa
     1081 ccagagcgaa agagaatatg ttgttaaaga ccgtaattag aattttacca aataattatc
     1141 ccgcccttaa cacaaaagaa tgaaaatcgt aattgtctca ccagcttatc cgttaagagg
     1201 cggaatagca aactttacag cacaactgta tcaagaactt tccttagaac ataaagttat
     1261 agtatttaat tttaagcgac agtaccctaa attatttttc cctggtaaaa cacaattaga
     1321 agttggagaa aatgttgata aaattccaag cagaatagaa attgattcaa tcaatccgat
     1381 tacctggatt aaaacagcaa ataatattat tgcggaaaaa cctgatcttg taatattcaa
     1441 atactggatg ccgttttttg cattgtgtta tggggtgatt gcaaaaaaaa ttaaaaaaag
     1501 aactaaagca aaaattatag ctgtctgtca taatatagtt ccacacgaaa aaaaacctgg
     1561 cgatgacttt ttgacaaaat attttttatc caggatggag tattttattc tattatcaaa
     1621 ccaggtggaa aatgatctga aaaaggttat ttccgatccc aaatcaaaaa tattgccgca
     1681 tcctatctac tcaagatttg gtagcgctgt tgataaacag tcggctttag attatctgaa
     1741 attaaaggaa tacaattact tacttttttt tggtttcatt cgtgattata aaggacttga
     1801 tgttttaatt gaagcaatga acttgattaa agaccaagat caaatcaagc ttattgttgc
     1861 tggtgaattt tatgaagatg aaaaaaaata tttagatctt attgcaaaat ataatttatc
     1921 aaacaagatt attttaatta aagattttat accaacttta gatgttaaat attatttttc
     1981 agtatgtgat gcagtagttt taccttatag agaagctact cagagtggaa ttgtacaaat
     2041 ggccataaat tttaataaac ccgtaattgc tgcaaatgtt ggcggagttt ctgaagtgat
     2101 agttaatgat gaaacgggtt atattgttga aaaagaaaat ccaaaggctt tagcagatgc
     2161 aattaagata ttttatactg caaataaaaa agaacttttc aacaaaaaca ttttacatct
     2221 aaaggataaa tattcctgga agtattttgt aagtggtcta tttgatctta ttaatacgta
     2281 agctttttaa tcttaaattt gaacc
//
LOCUS       DASWBY010000708         3985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_216318, whole genome shotgun sequence.
ACCESSION   DASWBY010000708 DASWBY010000000
VERSION     DASWBY010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216318"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VF870_12935"
     CDS             complement(<1..963)
                     /locus_tag="VF870_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HEX9253144.1"
                     /translation="MKTFLSTALLFLITLLLGCSSSSQMINISELEYPGDLKVITRAE
                     WGWTPLDRALPQQTITKITIHHGGEEFAEDKDVIQYLRNLQSWSRSEKKWIDIPYHYI
                     IDLKGNIYEARPINYPGDTNTGYNVTGHALICVVGNYEVQKLSEASLKSLVKLIAFLK
                     QKYHVEQKDIKGHKDYSNQTVCPGKDLYKYLADGSFLKMVDSELNPASEIVSSKVKTG
                     IEVLRDRNFDVLKGKRVGLVTNPTGVDSKLKSTIDILFEAKDVNLTALYGPEHGVRGN
                     YSAGDFVDFYIDDATKLPVYSLYGKTRKPTSDMLQEVDVLVFDIQ"
     gene            complement(978..1832)
                     /locus_tag="VF870_12940"
     CDS             complement(978..1832)
                     /locus_tag="VF870_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX9253145.1"
                     /translation="MIKFNDLSVETAEKILNSEVSSSLRDFFNTPLNISDEQIRNYGA
                     LGFVKIENVIEKDFLPEVKKIITSAVLLRKGKDERELKDKSQYEQSFMQCGFLCWDYP
                     AIKEFVFSKRFAGIAKDLLKAEHVRLWHDQALFKEPGGRITDAHQDCSYWPIAEPQFT
                     TTMWLALTDVPVEKGCLYFYPRTNDPNLKEYVDIFKNPHQPKLLRDSEKVFVPLKAGD
                     ATFHSGLTFHGAGNNQTTEMREAMTVIYIKDGTTFDANDERNKTHTSCVGLNDLEVIN
                     TKFTPILI"
     gene            2025..3512
                     /locus_tag="VF870_12945"
     CDS             2025..3512
                     /locus_tag="VF870_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEX9253146.1"
                     /translation="MEHYGFWSLLPPVLAIIFAIKTRQVFVSLLFGIWLGWIILSNGN
                     FLTGTIATIQALVNVFADASNTRTIMFSCLVGALIAFIQRSGGVSGFVNLINRFLIFL
                     ENKKIGNSRKVVQLLAWATGTAIFVESSINTLTVGTVFRPIFDRLKIPREKLAYIGDS
                     ISAPTCILIPLNAWGAYIMGLIAAQGFSSPLTVLIYAFPLNFYPMLAMGMVLFVILSG
                     KDFGPMKKAEERALKEGKVIRDGAVPLISADVVSLEKKENVKARAMNMIIPIAVMVGM
                     MPLMLMFTGWGQVENISSYPWYEQIFFAIGKGSGSTAVLYSVLTSIIVASILYGTQKI
                     FTFAETIDLIFKGISGLIPLALLMMLAFAIGTVCRELGTGIYTAELSKQWLAPDFVPV
                     IIFLVGCFIAFSTGTSWGTFAIMLPIGIPMAQALDANLYLTVAAALGGGVFGDHCSPI
                     SDTTIISSMASASDHIDHVKTQLPYALTAGAITAVLYLILGIIIH"
     gene            3659..>3985
                     /locus_tag="VF870_12950"
     CDS             3659..>3985
                     /locus_tag="VF870_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX9253147.1"
                     /translation="MKYLIGMDGGGTKTKCVLTDFSLNKIYETAGSASNFLVIGAETV
                     SETMLNLINDCATSQKISIDDIEAIVLGTTGGGRRNDAELLENQIFADAKQKSIPINK
                     FRVESDA"
BASE COUNT         1198 a          721 c          715 g         1351 t
ORIGIN      
        1 ctgtatatca aaaactaaaa catcaacctc ttgtaacata tccgaagttg gtttgcgggt
       61 tttgccatac aaagaatata cgggaagttt tgtcgcatca tcaatataaa aatctacaaa
      121 atcacccgct gaataatttc cgcgtacgcc gtgctcaggt ccatacaatg ctgttaggtt
      181 tacatctttt gcttcaaaca gaatatcaat tgtggatttt aatttgctat ctactccggt
      241 tggatttgtt actaatccga ctcgttttcc ttttagtaca tcaaaatttc tatcacgtaa
      301 aacttcaatg ccggttttta ctttagaaga tacaatttca gaagccggat ttaattcaga
      361 atcaaccatt ttaagaaaag aaccatcggc taaatatttg tataaatctt tacccgggca
      421 gacagtttga tttgaataat ctttatgccc tttaatatct ttttgctcaa cgtgatattt
      481 ttgcttgaga aatgcaatta actttactaa tgattttaac gatgcttcac taagtttttg
      541 aacttcataa tttccaacaa cacaaataag cgcatgaccg gtgacattgt aaccagtgtt
      601 tgtatcaccg ggataattta tcggacgtgc ttcataaatg tttcctttaa ggtcaattat
      661 ataatggtag ggaatatcaa tccatttttt ttctgacctg ctccagcttt gaagattgcg
      721 tagatactga atcacatctt tatcttctgc aaattcttct ccgccgtgat gaattgtaat
      781 tttagtaatt gtttgctggg gcagcgcccg gtctaatgga gtccatcccc attcggctct
      841 tgttattact tttaaatcac ccggatattc cagttcggaa atattaatca tctgtgagct
      901 tgatgaacat cctaacagga gcgtgatcaa aaataataaa gcagtactta aaaaagtttt
      961 catagaatat ttcttttcta gattagtatt ggagtaaatt ttgtattaat aacttccagg
     1021 tcatttaggc ccacgcacga agtatgagtt ttattccttt catcatttgc atcaaacgta
     1081 gttccatctt tgatatagat gacagtcata gcttcacgca tttctgttgt ttgattatta
     1141 cctgcaccat gaaaagttaa acccgaatga aatgttgcat ctccggcttt aagcggtaca
     1201 aaaacttttt ctgaatctct taaaagtttt ggctgatgcg gattcttaaa aatgtctaca
     1261 tactctttaa ggtttggatc attggtccgc ggataaaaat ataaacaacc tttttcaaca
     1321 ggtacatctg taagagcgag ccacattgta gttgtaaact gcggttctgc gataggccaa
     1381 taactgcaat cctgatgtgc atcggttatt cttccacccg gttctttaaa caatgcctga
     1441 tcgtgccaga gccttacatg ctcagctttc aacagatctt ttgcaattcc ggcaaatctt
     1501 tttgaaaaga caaattcttt aatcgcagga taatcccagc ataaaaatcc gcattgcata
     1561 aaagattgtt cgtactgtga tttatctttt aactcccgtt catctttacc ttttcttaac
     1621 agcacggcgg acgtaataat tttttttacc tcaggtaaaa aatctttttc aattacattt
     1681 tcaatcttaa caaaaccaag agcgccatag tttctaattt gttcatcaga tatgttaagc
     1741 ggagtattaa aaaaatctct gagcgaagag ctaacctcag aattaagaat cttttctgca
     1801 gtttcaacag acagatcgtt aaattttatc atatattttt aattaagtat ttatttagat
     1861 aaaaattaca attgcaattt acagtataag aattcttact tcaatcgatt tttgttttat
     1921 ctccattata atttcaatat tttgcaaaca gaatttttag atgttcgatt taataatgtg
     1981 caatgttttt ataaaaatta tctttaatta aaatcggctg atttatggaa cattatggat
     2041 tctggtcttt gctgcctccc gtacttgcaa taatttttgc aatcaaaacc cgtcaggtat
     2101 ttgtctcgct tctttttgga atctggctcg gctggattat cctgagcaat ggaaattttt
     2161 taaccggaac gattgcaact attcaggcgc ttgttaatgt gtttgccgat gcaagtaata
     2221 caagaacaat tatgttcagt tgtcttgttg gagctttgat cgcattcatt caaagatcag
     2281 gcggagtttc agggtttgta aatctgatta acagatttct aatttttctg gaaaacaaaa
     2341 aaattggaaa tagccgcaaa gtagttcaac tattagcgtg ggctaccggt actgcaattt
     2401 ttgttgagtc aagtattaac actcttactg tcggaactgt tttccgacca atctttgata
     2461 gattaaaaat tccccgcgaa aaattagcat acattggcga ttcaatttct gcgccgactt
     2521 gcattttaat tccattaaac gcgtggggtg cttacataat gggattgatt gccgcgcagg
     2581 gattcagcag tccattaaca gttttgatat atgcatttcc gctcaacttc tatccaatgc
     2641 ttgcaatggg aatggtttta ttcgtaattc tttcaggtaa agattttggt ccgatgaaga
     2701 aagcagaaga acgcgcgctt aaagaaggga aagttattcg cgatggtgca gtgccgctta
     2761 tctctgctga tgtcgtatca cttgaaaaaa aagaaaacgt aaaagcaaga gctatgaata
     2821 tgattattcc tatcgcagta atggttggaa tgatgccatt aatgctgatg tttaccggtt
     2881 ggggacaagt ggaaaatatt tcatcttatc catggtatga acaaatattt tttgcaattg
     2941 gtaaaggatc aggatcaacc gctgtgctat actcagtttt aacctcaata attgttgctt
     3001 ctatattata cggcacacaa aaaattttca cattcgcaga gactattgat ctgatcttta
     3061 aaggcataag cggattaatt cctttagcac ttttaatgat gcttgctttt gctattggaa
     3121 cagtttgccg tgaattagga accggtattt atactgcaga attatcaaag caatggttgg
     3181 ctccggattt tgttccggta ataatatttc ttgttggatg ttttattgca ttttcaactg
     3241 gaacatcgtg gggaactttt gctatcatgc ttccaatcgg tattcctatg gcacaagcac
     3301 ttgatgcaaa tttgtactta acggttgctg cagcattagg cggcggagta tttggcgatc
     3361 attgttcacc aatttctgat acaacaatta tttcatcaat ggcatcggca agtgatcaca
     3421 ttgatcacgt aaagactcaa ttaccgtatg cacttactgc aggcgctatt actgcggtat
     3481 tgtatttaat acttggaata ataattcatt aaaattttgt ttgtcattcc gggccagacc
     3541 cggaatcttg gcgatttaaa tgcaaaccaa agaatgttta gttggtcaat aagtttatta
     3601 aaggaattaa aaactttatc ttgcacaaga attttataaa aattatcagg tgaccacagt
     3661 gaaatattta atcggaatgg acggcggggg aacaaaaaca aaatgtgttc ttaccgattt
     3721 cagtttaaat aagatctatg aaaccgctgg atccgcatca aacttcttag taatcggagc
     3781 agaaactgtt tctgaaacta tgcttaatct tataaacgat tgtgcaacgt ctcaaaaaat
     3841 ttcgatagat gatattgagg ctattgttct tggaaccacc ggcggcgggc ggcgtaatga
     3901 cgctgagctc ttagaaaatc aaatctttgc cgatgctaaa caaaaatcta ttcctattaa
     3961 taaattccgg gttgaaagcg atgcg
//
LOCUS       DASWBY010000709         3846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_30755, whole genome shotgun sequence.
ACCESSION   DASWBY010000709 DASWBY010000000
VERSION     DASWBY010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30755"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..811)
                     /locus_tag="VF870_12955"
     CDS             complement(101..811)
                     /locus_tag="VF870_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9253148.1"
                     /translation="MKKIVYRSEDRGKANYGWLDTKYSFSFANYYNPKMMNFGMLRVL
                     NDDTVVPAMGFGTHPHDNMEIITLILEGALEHKDSMGTGSVIYKDEVQVMSAGSGITH
                     SEFNPSDKEAVKLFQIWIFPKEKNIKPRYDQKSFPISERKNKLIPAVSGLNKEGSLYI
                     HQDAEIYLGNFEKGKKVNYQISNPANGAYIFVIDGSLKVGDEELFKRDAIGISEVNNF
                     EIETLEDSNFLIIDVPMG"
     gene            963..>3846
                     /locus_tag="VF870_12960"
     CDS             963..>3846
                     /locus_tag="VF870_12960"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX9253149.1"
                     /translation="MKEILFISKNTNISFLIILALVCLFSTSVLPQDYIIKQLRIEDG
                     LSQSTIFSSLQDSQGYMWFATRSGLNRYDGYNFKIYFNDPKDPTSLSDDGTNSLYEDR
                     NGNLWIGTIYGNINRFERTTETFVYKNISDLVELRPDQTDDFYEYPLSFSRNQKTTIT
                     SIAEDKDGKLWIGTWGNGLIVIDKNFKKYNHFFYDKNNPNGLRTNRIMDLNFDSDGRL
                     WIATFGGGVTRISKSINDSKERFSFETLLQGNNDFSLSDNKLLNLFEDSEKNIWIGSY
                     YGGLMFIPADQTKLPFGKMKINCQRCPMSKSSLSRNTIMAFAEDKEHYLWIGTFGGGL
                     IRYDKNKNESLHFFNDPMNENSLGDNDVLSLCTDRSGIIWAGSHLGAGITKIYKNNTR
                     FNHVKHLPRKKNTLNDDVVWAIYKDKDNILWIGTYKGGINRFDISSNNFSYLVKSDFK
                     KSISSNHVRAIEEDRFGNLWVGTYDGGLNIINKNTYRTIVYKNDPNNRTSIGGNQVQD
                     ILIESDSVYWIATFGGGLNKVVVKGNPLNQQLKFERYKNVKDDINSLSDNRVYKLYLS
                     RDGVFWICTYGGGLNSFDPVTKKFKRYPISSDRENKFNIQNLMTILEDSDGLLWLGSY
                     GGSLTSFNRGTEEFKQYSFQDGLTSGVVYGILEDNSKNLWISSDNGIFKMNLADKEFK
                     RYDIQDGLQSLEYSGGAYFKDADGILYFGGINGFNYFDPAKIKSNTYIPPIVITSIKV
                     LNEYVKGDHKELILNHKNNFITFEFSSLDYTDPRDNQYSYILEGLQDDWQLAESSSRS
                     ATYTDLRPGTYVFKVRGSNSDGMWSDNYASIKIKILYPFWETWWFITLMIILLAAFIY
                     YLGTMKTKNLLAIEKLKSKLAADLHDNIGSGLTEISILSEVASRKNNSEQKIFGGELN
                     KISELSRQLVDNMSDIVWVVNPSRDSLHDLILRLKDTYSELLNSMGI"
BASE COUNT         1289 a          619 c          674 g         1264 t
ORIGIN      
        1 tttgcaccac tgctccctaa agaaaaagcg gtccttcgtc tgtcacgatc gaaccgtaga
       61 atgaagtcac ggaatcgtcc gtgacttccg ttaaaaatta ttaacccatc ggaacatcaa
      121 taatcaaaaa gtttgaatct tcaagagttt ctatttcaaa attatttact tcagaaattc
      181 caatcgcatc acgtttaaat aattcctcat caccaacttt taaacttcca tcaattacaa
      241 aaatgtaagc accatttgca ggattgctta tctgataatt tactttttta cctttctcaa
      301 aattcccaag ataaatttct gcatcctgat gaatatataa cgaaccctct ttatttaatc
      361 cggaaactgc gggaataagt ttattctttc gctcagaaat aggaaatgat ttttgatcat
      421 accttggttt tatatttttt tcttttggaa aaatccatat ctgaaaaagt tttaccgctt
      481 ctttatcaga tggattaaac tctgagtgtg taattcctga tcccgcagac ataacctgaa
      541 cttcatcttt ataaataaca gaaccagtgc ccatactgtc tttgtgttca agcgcgcctt
      601 ctaaaatcaa agtaattatt tccatattat cgtgcgggtg agttccaaat cccattgccg
      661 gaacaacagt atcatcatta agaacacgca gcattccaaa gttcatcatt ttaggattat
      721 aataattagc aaagctgaaa ctgtattttg tatccagcca tccgtagttt gcttttcccc
      781 tgtcttcgga tctataaact atttttttca tcgttacctc tttttgtcta tttttttaat
      841 tacaatgtat gtcataacac gtttttagtc aaatatccag atcacatatt gcacacagaa
      901 atattttggt gatatagaat ctttgcagaa aatatcctat atatggttaa gaacttagta
      961 aaatgaagga aatcttgttt ataagtaaaa acacaaatat atcttttcta atcattctgg
     1021 cacttgtgtg tttgttttcc acgagtgtct tgccgcagga ttatattatt aagcagcttc
     1081 gcatagaaga tggtttatca caaagtacaa tcttttcttc tttgcaggat agccagggat
     1141 atatgtggtt tgcaaccaga agcggattga atcggtatga tggatataat tttaaaattt
     1201 actttaacga tcccaaagac cctacttccc tttcagatga tggaaccaac tcgctttatg
     1261 aagatagaaa tggaaatctg tggattggaa caatttacgg caacataaac agatttgagc
     1321 gaacaactga aacatttgtt tataaaaata tttctgattt ggttgaactc cgtcctgatc
     1381 aaacagatga tttttatgaa tatccgcttt ccttttcaag aaatcaaaaa accactatta
     1441 cttctatcgc agaagataaa gacggtaagt tatggattgg aacctggggc aatggcttaa
     1501 tagtaattga taagaacttt aaaaagtaca atcatttttt ttatgataag aataatccaa
     1561 acggacttag aactaatcga ataatggatc tgaattttga tagcgatgga agattgtgga
     1621 ttgctacgtt tggcggcgga gtaacaagaa tttcaaaatc aataaatgat tctaaagaga
     1681 ggttttcttt tgagactttg ctgcagggaa ataatgattt ttcactttcc gataataaac
     1741 tactcaattt atttgaagat tctgaaaaaa atatctggat tggctcctac tacggcggat
     1801 tgatgtttat acctgctgat caaacaaaat taccttttgg caaaatgaaa attaattgtc
     1861 agcgttgtcc tatgtcaaaa agtagtttaa gtcgtaatac aattatggct tttgctgaag
     1921 acaaagaaca ttatttgtgg atcggtacat ttggcggagg actgataaga tacgacaaaa
     1981 ataaaaatga gtcacttcat ttttttaatg atcctatgaa tgaaaattct ttaggtgata
     2041 atgatgtcct ttcgctttgc actgatcgct caggaataat ttgggctggc tctcatcttg
     2101 gagctggaat aacaaaaatc tacaaaaaca atacacgctt taatcacgtt aaacacctgc
     2161 caagaaaaaa gaatacttta aacgatgatg ttgtttgggc tatttataaa gacaaggata
     2221 atattctttg gattggaaca tataaaggcg gaataaatcg atttgatatt tcttcaaata
     2281 atttttcata cctcgttaaa tcagatttta aaaaatcaat tagcagtaat catgttagag
     2341 cgattgagga agacagattt ggtaatttat gggttggcac ttatgatggc gggttgaata
     2401 tcattaataa aaatacttac agaacgattg tttacaaaaa tgatcctaat aatcggacaa
     2461 gcattggtgg aaatcaggtt caggatatat tgatagaatc agacagcgtt tattggattg
     2521 ccacgtttgg cggtggatta aataaggttg ttgttaaggg aaatccatta aatcagcaat
     2581 taaaatttga acgatataag aatgttaaag atgatataaa ctcattaagc gataaccggg
     2641 tttataaatt atatctttcg cgtgatggag ttttttggat ttgcacatac ggtggcggac
     2701 tcaattcctt tgatcctgtt actaagaaat ttaaaagata tcccattagc tcagatcgag
     2761 aaaataaatt taacatacag aatcttatga caatacttga agattctgat gggttactat
     2821 ggcttggttc ttacggcggc agcttaacaa gttttaatcg agggaccgaa gagtttaaac
     2881 aatactcttt tcaggatgga ttaaccagcg gagttgttta tggaatatta gaagataatt
     2941 caaaaaatct ctggataagt tccgacaatg gaattttcaa aatgaatctt gctgataaag
     3001 agtttaagcg atatgatatt caagacggac ttcaaagttt agaatacagc ggtggcgctt
     3061 actttaaaga tgctgatggc atattatact ttggcggtat aaatggtttc aactattttg
     3121 atccggctaa aataaaatcc aacacttata ttccaccaat agtaataacc tctataaaag
     3181 ttcttaatga atatgttaaa ggtgatcata aagaattgat tcttaatcat aagaataatt
     3241 ttatcacctt tgaattctca tctttagatt atactgatcc tcgtgataat cagtactcat
     3301 atattctgga aggtttgcag gatgactggc aattagcaga atcatcatca cgcagtgcaa
     3361 catacacaga tctaaggccg ggtacttacg tatttaaagt ccgtggttct aacagtgatg
     3421 gaatgtggag tgataactat gcttcaatca aaataaaaat cctttatcca ttctgggaaa
     3481 cctggtggtt tatcacgctt atgataattt tattagcagc atttatatac taccttggta
     3541 ctatgaagac aaaaaacttg cttgcaattg aaaaattaaa atctaaactt gccgcagatt
     3601 tacatgataa tattggttcg ggattaaccg agatttcaat tttaagtgaa gttgcatcaa
     3661 gaaaaaataa ttccgagcaa aaaatatttg gcggagaact aaataaaatt agtgagttat
     3721 cccggcagct tgttgataat atgagcgata ttgtgtgggt tgtaaaccct agtcgtgatt
     3781 ctttacacga tctgattctt cgcttaaaag atacttacag tgaactgctt aactcgatgg
     3841 gaattt
//
LOCUS       DASWBY010000710         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_709327, whole genome shotgun sequence.
ACCESSION   DASWBY010000710 DASWBY010000000
VERSION     DASWBY010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709327"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VF870_12965"
     CDS             <1..582
                     /locus_tag="VF870_12965"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX9253150.1"
                     /translation="SYLPKYRGAAPIQWALINGDTETGLTTFKLAERVDTGNIYLQEK
                     VVIYPSDNFETLHDRMSLIGADLVLKTVELIQNGNFELKQQNDSLASPAPKIRKEICL
                     IDFNKSAEEIHNLVRGLSPHPAAFFNQNNKVIKIYKSEVARGINLLPFQFHQTRTELL
                     VGCGKDALRILEIQQEGKKRMSIEEFLRGFRIF"
     gene            705..>1893
                     /locus_tag="VF870_12970"
     CDS             705..>1893
                     /locus_tag="VF870_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9253151.1"
                     /translation="MKYKNNILEQIGNTPLIKINKINKGLKPQIFAKLESANPGGSVK
                     DRIGYAMIVDAEKRGVIKPGGTIIEATSGNTGIGLALTAAVKGYKSVFVVTSKVSDEK
                     INYLKAFGAEVIVVSNLADPDSPEYYVNVAKRLAKEIPNSFFAYQYSNPSNPEIHYQT
                     TGPEIWEQTDGKITHFISSIGTGGTISGTGKYLKEKNPDIKVIAADPLGSIFKHYKET
                     GELISGTPYLVEGIGQECLPENVHFQYIDKIYNISDKESFSAARRLTKEEGIFCGGST
                     GTIVHVALEVAKSCSENDVIVFIVCDTGERYLTKVHNIEWLKQNRMLEPEVRTLRDLS
                     DIKKQKGVEEIVSVKETDFVKDVLQLISKTGYSQIPVMEGRQSIGCIRENRLLTKLVE
                     NPLL"
BASE COUNT          699 a          277 c          367 g          550 t
ORIGIN      
        1 tcatatcttc caaaatatcg aggtgcagcg ccaatacaat gggcattgat taatggagat
       61 actgaaacag gcttaactac atttaaactt gctgagagag tagatacagg aaatatttat
      121 cttcaggaaa aagtggttat atatcctagt gataattttg aaactcttca cgatcgaatg
      181 agtttaattg gcgcagattt agttttaaag actgttgagc taattcaaaa cggtaacttc
      241 gaattaaagc agcagaatga ttcactcgct tcaccagcac ctaagattag aaaagaaatt
      301 tgtttaatcg attttaataa atcagcagaa gaaattcata atcttgttag aggattatca
      361 cctcatccgg cagccttttt taatcaaaac aataaagtga ttaaaatata taaatcagaa
      421 gtagccagag gcataaacct gctacctttt caatttcatc aaaccaggac agaactttta
      481 gtaggatgcg gaaaagatgc gcttagaatt cttgaaatcc agcaggaggg aaagaaaaga
      541 atgagcatag aagaattctt aagaggattt agaatcttct aatctgataa atgctaagtt
      601 gaattaatca atgtaagata aatcactatc aggtttttaa actttcatca ttcaaatcat
      661 ttctttaaaa ttaccacagc taattcgaac agtgagcaaa aattttgaaa tacaaaaaca
      721 acatacttga acaaattgga aatactccat taattaagat taacaaaatc aataaaggtt
      781 taaagccgca gatatttgca aaacttgaat ctgcaaatcc gggtggaagt gtaaaagacc
      841 gcattggtta tgcaatgatt gtagatgcag agaaaagagg tgttataaaa cccggcggta
      901 ccataattga agcaacaagt ggcaacactg gaattgggct tgctttaacc gcagcagtta
      961 aaggttataa atctgtgttt gttgttacat caaaagttag tgatgaaaaa ataaattacc
     1021 ttaaagcttt tggagccgag gttatagttg tatcgaattt agctgatccg gatagtcctg
     1081 aatactatgt taatgttgca aaaagattag ctaaagaaat tccaaattca ttttttgctt
     1141 atcaatattc taatccgtca aaccctgaga ttcattatca aacaaccgga cctgaaattt
     1201 gggaacagac tgatggaaag ataactcatt ttatttcgag tattggaact ggcggaacaa
     1261 ttagcggaac gggaaaatat ttaaaagaaa aaaatccaga tataaaagta attgccgctg
     1321 atccgcttgg ctctatattc aagcactata aagaaacggg tgagttaatc agcggtacac
     1381 catatcttgt tgaaggtatt ggacaagaat gtttgcctga gaatgttcac ttccaataca
     1441 ttgataagat ttacaatatt agtgacaaag aatctttttc tgctgcgaga agattaacta
     1501 aagaagaagg aattttttgt ggtggaagta caggaacaat cgttcacgtt gctttagaag
     1561 ttgcaaagag ttgttctgaa aatgatgtaa tcgtttttat agtttgtgat actggtgaaa
     1621 ggtatttaac aaaagttcac aacatagaat ggctgaagca gaatagaatg cttgaaccag
     1681 aagtaagaac tttgcgtgat ctatcggaca tcaaaaagca aaagggagtt gaggaaattg
     1741 tatcggtgaa agaaacggat tttgtaaaag atgtacttca actgataagt aaaacaggtt
     1801 attcacaaat tcctgttatg gaaggaagac aatctatagg ttgtatcaga gaaaatcgtt
     1861 tgcttacaaa gttggttgaa aatcctttgc tat
//
LOCUS       DASWBY010000711         3688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_216548, whole genome shotgun sequence.
ACCESSION   DASWBY010000711 DASWBY010000000
VERSION     DASWBY010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216548"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF870_12975"
     CDS             complement(<1..279)
                     /locus_tag="VF870_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9253152.1"
                     /translation="MTNYSVEAVDLNKSFGRRLIFNDLKFNFNKAGVYGISGPNGSGK
                     STLVKIIAGIIGASKGKIVHKLSDKEISEEHLHDHIGFVSPYLVLYEEF"
     gene            complement(272..550)
                     /locus_tag="VF870_12980"
     CDS             complement(272..550)
                     /locus_tag="VF870_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253153.1"
                     /translation="MPVKKSAANTIYIAFQSNDAIGGEVDLNIYSAGVELYYSGKKNI
                     QSAYSKGSTKYCEISLDKSEFDYPSGVYIYVLKSGEDIFKGKLVIFND"
     gene            complement(674..1966)
                     /locus_tag="VF870_12985"
     CDS             complement(674..1966)
                     /locus_tag="VF870_12985"
                     /inference="COORDINATES: protein motif:HMM:NF045524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6640 family putative metalloprotease"
                     /protein_id="HEX9253154.1"
                     /translation="MNKFIIAVFILLISTTILPQEASKNELDSLYNLFTFIKGVNPSD
                     KLQKQFEQDPAIKKCGMELVNTLSQNINSFSIEKQMVLSSILERPQMQTSTITPNGFF
                     RVHYDLTGTNALGYDLNSLLQALDSAYSFEINYLGYSAPPPDGVDGGDDKYDIYVQNL
                     SGLYGYTQFETKISDSRFTSFMVIDNDYTGYYSTGIKGARVTVAHEFHHGIQGGNYAP
                     AGSNSPFRNSDVFYYEITSTAFEEFVFDDVNDYYAYLPSYFQHPERAMPLNDGYNLAI
                     WNIYLQNNFGFDILKRQWDLIPVNDALKAIAISISENGSTFGNELNKFGIWTYFTNSR
                     NIYSGGYFEEASNYPLISPTATMQYSPPFKNYNINVNPTANFFLRLNLPSPDGVFFTI
                     ITNSDWQSAIDDPNQVFPATYYMFSDTVTGTKVISDKY"
     gene            complement(1963..>3688)
                     /locus_tag="VF870_12990"
     CDS             complement(1963..>3688)
                     /locus_tag="VF870_12990"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX9253155.1"
                     /translation="TGNPSLSAQIKFNSYPENISTVNNSDNSQSFLVSGNSFDGLSVL
                     SYRNNTLKEIKVSQKHIFQNAQFIDLNSDGFEDIVALNSIENKLHLFFNNSHGDYNEL
                     RYLNISSDVLSLKVFDVNYDQFKDIIVSTTTGVIIYFGDASASFEKSVEVRTSYAVDK
                     FIIGDFNHDGFFDINYLNVEEGIISTIFAKDFDLFYPEFIQKKQKGIVDVIPFFSKFV
                     YGAAYLNSNGQVHIISSVSAMSEDQQLAIGIEPNLITSFDHVNNGITDIAFTDDYDKR
                     LKIIIRNNAGLPERLFAVNLYEEHNKILDFSNSKSVKTFFLYSINKKIIESIEIDFEK
                     FSFIRKFHYAEGPIKDLIIKADSKGDAELFILYSKNNNLNLQVITKTQINYNQKLYTN
                     LSSNWFDPFLFFDDQLTIGYWQFESNYIKLNFLNFSDKDRKPVNELKLNRIDTLLVTK
                     SNQSSSKSDLKYSAVLSNGEEILLATGGAKVKLFKAKNTKNGFRIQDKNQLFFGKTNS
                     VFVYNSNLSALTEFKPAKTGNKLFVSNIFDDLELNNYNIQKLDQRNYHLIFTNIKSGN
                     IEIRQLSR"
BASE COUNT         1248 a          605 c          550 g         1285 t
ORIGIN      
        1 aaactcttca tataaaacaa gataaggcga aacaaatccg atgtgatcgt gtaaatgctc
       61 ctcagaaatt tctttatcac taagcttgtg aacgattttg ccttttgagg caccaattat
      121 tcctgcaata atttttacta gggtagattt tccagatcca ttgggaccgg aaattccata
      181 gacacctgct ttattaaaat taaacttcag atcattgaag attagtcttc ttccaaagga
      241 tttgttaaga tcaacagctt caacggaata gttagtcatt aaagattacc aactttcctt
      301 taaagatatc ttcgccggat tttaaaacat aaatataaac tcctgaagga taatcgaact
      361 ccgacttatc cagcgaaatt tcacaatatt tagtggaacc ttttgaatat gcagactgaa
      421 tatttttttt acccgaataa tataattcta ctcccgcaga gtaaatattt aaatcaacct
      481 ctccgccaat tgcatcatta gactggaacg ctatataaat tgtattagca gcagacttct
      541 taaccggcat tgggtatgcg taagtttcac cctctaaatt tggaattgaa taacttgaat
      601 cgccataaac aacaatgtta tttaatatac ctgcgttgtt ccagaatgtc tgattatctt
      661 tattaaaact aatttaatac ttatcgctaa ttacctttgt gcccgttaca gtatcactaa
      721 acatgtaata agtagccggg aaaacctgat ttggatcatc tattgcgctc tgccaatctg
      781 aatttgtaat tattgtaaaa aaaacaccat ccggtgaagg cagatttaat cttaagaaga
      841 aattagcagt tggattaaca tttatattat aatttttaaa cggtggtgaa tattgcattg
      901 tagcagttgg acttataagc ggatagttac tcgcttcttc aaaatacccg ccggaataaa
      961 tatttcttga atttgtgaag taagtccaga ttccaaattt attaagttcg ttcccaaatg
     1021 ttgatccatt ctcactaatg ctgattgcga tagctttgag tgcatcatta acaggaatta
     1081 aatcccattg tctttttaat atatcaaaac caaaattgtt ttgaagataa atattccaga
     1141 tagctaaatt gtatccgtca tttagtggca ttgctctttc aggatgctga aaataagaag
     1201 gcaagtatgc gtaataatca tttacatcat caaaaacgaa ttcttcaaat gctgttgaag
     1261 taatttcata ataaaaaaca tctgaatttc tgaaggggga attactgccg gcaggagcat
     1321 aattaccgcc ctgaattcca tgatgaaatt catgagccac agtaactctt gcacccttta
     1381 ttccggttga ataatatcct gtataatcat tatcaataac cataaatgat gtaaatcggg
     1441 aatcagaaat tttggtttca aattgagtgt agccataaag tcctgataga ttttgtacat
     1501 aaatatcata tttatcatca ccgccatcta caccgtctgg aggtggggct gaataaccaa
     1561 ggtaattaat ttcaaaactg taagctgagt ccagagcttg cagcaatgaa tttaaatcgt
     1621 aacctaaagc attagtaccc gtcaaatcat aatggactct aaaaaaacca tttggagtaa
     1681 ttgtgcttgt ctgcatctgt ggtctttcca gtatggaaga caataccatc tgcttttcaa
     1741 ttgaaaaact atttatgttt tgactgagtg tgttaaccaa ttccattccg cattttttta
     1801 ttgctggatc ttgctcaaac tgcttttgca atttatcgga aggattaaca ccttttataa
     1861 aagtgaacag gttgtacaat gaatcaagct catttttcga tgcttcctga ggaagaattg
     1921 ttgttgatat caagagaata aaaacagcta taataaattt attcatcttg atagctgcct
     1981 tatttcaatg ttaccagatt taatgtttgt aaatatcaaa tgataatttc tctgatctaa
     2041 tttttgaata ttgtaattgt ttaattctaa gtcgtcaaat atatttgaca caaataattt
     2101 gttacctgtt tttgcaggtt taaattctgt taacgcactt aaattagaat tgtaaacaaa
     2161 aaccgagtta gttttaccaa aaaataattg atttttatct tgtatcctaa atccattttt
     2221 ggtgtttttt gctttaaaca gtttaacttt tgcgccgcct gttgcaagta atatttcttc
     2281 tccgtttgat aaaacagctg aatatttaag atcagatttg cttgatgact ggttggattt
     2341 tgtaaccaac aaagtgtcta tccggtttag ttttaattca tttaccggtt tacgatcctt
     2401 atccgaaaaa ttaaggaaat ttaattttat ataattgctt tcaaactgcc agtaaccgat
     2461 ggtcaattgg tcatcaaaaa acaaaaaagg atcaaaccaa tttgagctta agtttgtata
     2521 aagtttttga ttataattta tctgtgtttt ggtaataacc tgaagattaa gattattatt
     2581 ttttgaataa agaataaaca attcagcatc acctttgcta tcagccttaa taatcaaatc
     2641 cttaattggt ccttctgcat aatgaaactt tctgataaaa ctaaattttt caaaatcaat
     2701 ttctatagat tcaataattt ttttattaat actataaaga aaaaatgttt taactgattt
     2761 tgaattgctg aaatctaata ttttattatg ttcttcgtat aaatttactg cgaacaatct
     2821 ttcaggcaag ccggcgttat tacgaataat tatttttaat cttttatcgt agtcatcagt
     2881 aaaagcaata tcagtaatac cattgtttac gtgatcaaaa gaagtaatta aatttggctc
     2941 aataccaatt gccagctgct gatcctcaga cattgcggat actgatgata taatatgaac
     3001 ctgaccatta ctatttagat aagctgctcc gtatacaaac ttgctgaaaa atgggattac
     3061 atcaacaatc cctttttgct ttttttggat aaactccgga taaaataaat caaaatcttt
     3121 ggcaaagatt gttgaaatga ttccttcttc cacatttaaa tagttaatat caaaaaaacc
     3181 atcatggtta aaatcaccaa taataaattt atcaacagca tacgatgttc taacttcgac
     3241 tgatttctcg aatgaagcgc ttgcatcgcc aaaataaatt attactccgg ttgttgtact
     3301 tacaattata tccttaaact gatcataatt aacatcaaaa acttttaatg ataaaacatc
     3361 actgctaata tttagatatc ttaattcatt ataatcccca tgcgaattat taaaaaacag
     3421 atgaagttta ttttcgattg aattaagtgc aacaatatct tcaaagccat cagaatttag
     3481 atcaataaac tgagcgtttt gaaaaatatg tttctgtgat actttaattt cctttagagt
     3541 gttattccga taagatagaa ctgataagcc atcaaaagaa tttccggata ctaaaaatga
     3601 ttgtgagtta tctgagttat ttacagtact aatgttttca ggatatgagt taaattttat
     3661 ttgagcagat aaagatggat tgcccgtg
//
LOCUS       DASWBY010000712         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_216599, whole genome shotgun sequence.
ACCESSION   DASWBY010000712 DASWBY010000000
VERSION     DASWBY010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216599"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VF870_12995"
     CDS             complement(<1..1045)
                     /locus_tag="VF870_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253156.1"
                     /translation="MNEQILNNLDKPKELEKLYRNNKTAFKKDFNSVYPDIKENTIAQ
                     IWNERLNFENEEISFGSNSELLFVIIASFIAGVIAKLPQFIGINPEYFYPRNISFVVF
                     PVLIVYFAWKHKTQTKILTGALLAVLISVLFINFLPNSQTSDTLILACIHLPLFLWAV
                     LGFTYLGEDLQNNQRRLDFLRYNGDLIVMTTIILIAGALMAGITLGLFRLIDIQVEEF
                     YFQYIGVWGLAASPIVATYLVQTNPQLVNKVSPVIAKIFTPLVLVTLVIYLIAVIYTG
                     KDPYNDREFLLIFNMLLLGVMAIIFFSIAETSKNSGGKISTLMLLALSIVTIIINGIV
                     LSAILFRISEWGLT"
     gene            complement(1432..2552)
                     /locus_tag="VF870_13000"
     CDS             complement(join(1432..2288,2288..2552))
                     /locus_tag="VF870_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611339.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX9253157.1"
                     /translation="MGKSKFTTEQIISKLREAEVLQSNGQSIAEICRHLGIKDQTYYK
                     WRKEYGGMRVDQAKRLKEVEQENTRLRKLVSDLSLDNAILKETVPGKLLSPEKKRRAV
                     EQVKEKLKVSERRVCSVLGQARSTQRRKVKTRDDEQALREEIIKLACEYGRYGYRRIT
                     ALLRNQGWKVNHKRVERIWRQEGLKVPQKQPKRRRLWLNDGSCIRLRALYPNHVWSYD
                     FVADRTSDGRPIRMLTIIDEYTRECLSIHTQRRIKAPEVLYKLSELFITKGVPDHIRS
                     DNGSEFSAKSVRGWLERLGVKTLFIEPGSPWENGYIESFNGKLRDELLNGEIFDNILE
                     ARVITEQWRKHYNKIRPHSSLNFRPPVPEVMIPHQYASA"
BASE COUNT          776 a          543 c          444 g          976 t
ORIGIN      
        1 gtgtaagtcc ccattctgaa attctgaaca gaattgctga aagcacaatt ccatttatga
       61 ttatagttac aattgaaagc gcaagcagca taagagtgct tattttgccg cctgagtttt
      121 ttgacgtttc cgcaattgag aaaaatataa tcgccataac tccaagcaac agcatattaa
      181 agatcaataa aaattctctg tcattatatg ggtctttgcc tgtatagatc accgcaatca
      241 aataaattac aagtgtaact aagactaatg gtgtaaaaat ttttgcgatt acaggtgaaa
      301 ctttattaac caattgagga tttgtctgaa caaggtaagt tgctactatt ggagatgcgg
      361 caagacccca aaccccaatg tattggaaat agaattcttc tacctggatg tcaatgagtc
      421 taaataatcc caaggtaata ccagccatta aagcacccgc aataagtata attgtggtca
      481 ttacaataag gtctccgtta tatctcagaa aatccagtcg tctttgattg ttttgaagat
      541 cctcacctaa ataggtaaag cccagcacag cccataaaaa cagtggcagg tgaatgcagg
      601 ccaggatcag agtatcgctt gtttgactgt tgggaagaaa attaataaag agtacagaaa
      661 ttaatacagc taataaagca ccagttaaaa tttttgtttg agtcttatgt ttccatgcaa
      721 agtaaactat taatactggg aagactacaa atgatatatt acgaggataa aagtactccg
      781 ggttgatgcc aataaattgc ggaagttttg caataactcc tgcgataaat gaagctatga
      841 taacgaataa taattcactg tttgaaccga aagaaatttc ttcattttca aagtttagcc
      901 tttcattcca gatttgcgca attgtgtttt ccttgatatc ggggtaaaca gagttgaaat
      961 cttttttgaa agcagtctta ttgttccggt acaatttttc aagctcttta ggtttgtcta
     1021 agttattcag gatttgttca ttcatttaaa tgctcttttt atttgatgca gaactgtgtt
     1081 gtttatctaa atggatttta atttataact ggtgaaagat ataaaataat tcaatgataa
     1141 aaagatagtt aatatttttt catagaataa attgaataat tactgcactc ctggacagtc
     1201 tggcaggtat agttttaaca atgcaaatta ctgattcata aattgcgtta tggaattgcc
     1261 ccctctgatc agcgcagaca gtaaaagtca aataaattaa aaagtaacaa ctttatctgc
     1321 ttccacagat aaaaaatatt ttttgctatt tatattaaga ttgtatctag gtattgtata
     1381 aatgacctgc ccctagtaat ggtaccaata cattagttag taaatccatc atcaagcact
     1441 ggcatattga tgagggatca ttacctccgg taccggaggt cgaaaattta acgaactatg
     1501 cggtctgatt ttattgtaat gttttctcca ttgttctgta ataactcttg cctctaaaat
     1561 attatcaaat atttctccgt tcaatagctc atcccttaat tttccattaa atgattctat
     1621 gtatccgttc tcccagggac ttcctggttc aataaataat gtctttactc ctaatctttc
     1681 caaccatcct cttactgatt ttgcactgaa ctctgatccg ttatctgagc gtatatgatc
     1741 cggaactcct tttgtaataa ataactctga aagtttgtaa agtacttccg gtgcttttat
     1801 tcttctctga gtatgtatgc ttaaacattc tcttgtgtat tcatctatga ttgtcaacat
     1861 tcttataggt cttccatcac ttgttctgtc tgctacaaaa tcataactcc atacgtgatt
     1921 tggatataaa gctcttagcc ttatacacga accatcattt aaccacagcc ttcttctttt
     1981 cggctgcttc tgtggcacct tgagtccttc ttgtctccat atcctttcaa cacgcttatg
     2041 gttcactttc catccctgat ttctcagtag tgctgttatc cttctatatc cataacgacc
     2101 atattcacag gctaacttta ttatctcttc ccgaagggct tgctcatcat ctcttgtctt
     2161 tacttttcta cgctgtgtag atcttgcttg accaagtact gaacatactc ttctctccga
     2221 tactttcagc ttttctttta cttgttcaac tgcccggcgc ttcttctccg ggctcagtag
     2281 tttccccgga ctgtctcctt taggattgca ttatccagtg acagatctga tacaagtttt
     2341 cttagtcttg tgttttcttg ttctacttct tttaatctct ttgcctgatc tacccgcatt
     2401 cctccatatt cttttcgcca tttgtaataa gtctgatcct ttatcccaag atgacggcat
     2461 atctccgcta tgctttgacc atttgactgc aaaacttcag cctcccgaag tttactaatg
     2521 atctgttcag ttgtgaactt gcttttgccc ataacttttt ctcctttgat cctaatttaa
     2581 taattggatc gcttttttgg gggcaggtca atcccaactt gtctctttat ctttccagcc
     2641 ggagatgatt tgtccacccc tgctttttga agtttcagaa gttatttttg gattatagga
     2701 atatttaagt tttttagaat tgctctccgt gtttgttgt
//
LOCUS       DASWBY010000713         1907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_30869, whole genome shotgun sequence.
ACCESSION   DASWBY010000713 DASWBY010000000
VERSION     DASWBY010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30869"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VF870_13005"
     CDS             <1..223
                     /locus_tag="VF870_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253158.1"
                     /translation="IQYAIPQQGNVSLKVYDILGKEVAILVDEFKSAGSYEVEFKSSV
                     SSRQLASGVYLYKIQSGDFVQTRKMILLK"
     gene            678..1748
                     /locus_tag="VF870_13010"
     CDS             678..1748
                     /locus_tag="VF870_13010"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX9253159.1"
                     /translation="MKKLIRLITIVLILLSVTLVAQDQSVKPITEINVHSLGPQKIGN
                     LPPVVVPPNNPQTSQKIQLGKLLYFDTRLSKDNTISCATCHDPAMGWSDAGPTSKGIN
                     GQMGGRRSPPVSNSAYNPLQFWDGRAPSLEEQAKGPVENPIEMGNTHEVMIRTVEDIP
                     GYVEIFKKVFNDSKLSLDMIADAIASFERTVVTTDSPFDRFVKGDNQSLTPLEKKGLE
                     IFNGKGHCTSCHYGGNFSDGRFHNLGVNPTDGSKPDEGRYVITKNPKDMGAFKTPTIR
                     DASLRAPYMHNGSEKTLEEVVEFYNVGGRLDDPNLDPLIVPLGLNEDEKKALVAFMTR
                     ALISTNPEVANVKPVTTSEFPK"
BASE COUNT          673 a          327 c          348 g          559 t
ORIGIN      
        1 tattcagtat gcaataccac agcaaggcaa tgtatcatta aaagtttacg acatactggg
       61 taaggaagta gcgattcttg ttgatgagtt caaatcagct ggaagttatg aagttgagtt
      121 taaatcctca gtcagcagtc gtcagttggc aagcggagtt tatttatata aaattcaatc
      181 tggagatttt gtgcagacac gcaagatgat attgttgaag taagagatgt caatcgaaaa
      241 acgtgaaatg ataaaaggcg attctacccg tcctgataac aggacgaggc aagcgaatcg
      301 cctttttatt gctatttaaa catacttgct aatctgcaat caattcttct tgacaatgat
      361 aaatatttaa atgagtttta catatataaa tatcatctac tccgagaggg ctaattattg
      421 taattatttt gttttaagtc ctgagatgac atctcgataa ctctcccgaa gcagacctta
      481 tattaagaaa tattttttca caaactaaat ttatttatca aactgatatt gtattaatta
      541 cttgataatt attaattgat ttatccttat atagactaaa ttgtatacac attcattatg
      601 aactaaataa gtttatatat catcagcaca taaaaacagg gaacaactta acaacagacc
      661 tactttaagg aggtaaaatg aaaaagttga tacggttaat aacaattgtt ctaatattac
      721 tgtcagtaac attagttgct caagatcaat cagtaaaacc aattactgaa ataaatgtac
      781 attcattagg accacaaaaa ataggtaatc ttcctccagt agtcgtacca ccgaataacc
      841 cccaaaccag ccaaaagatt caactgggaa agttgctata ttttgatact agactctcaa
      901 aggataatac tattagctgc gcaacgtgcc atgatccagc aatggggtgg tctgatgcag
      961 ggcctaccag caaaggtatt aatggtcaaa tgggtggacg aagatctccc ccagtatcaa
     1021 actctgcata taatccacta cagttttggg atggtagagc tccaagccta gaagaacaag
     1081 caaaaggtcc tgtagaaaat cctatcgaaa tgggtaacac acatgaagtt atgatccgga
     1141 cagtagaaga tattcctggt tacgttgaaa ttttcaagaa agtatttaat gattcaaaat
     1201 taagtttaga tatgatagcc gacgctattg catcatttga acgaacagtt gtaacaactg
     1261 attcaccttt tgatcggttt gttaaaggtg ataatcaatc gttaacccca ctcgagaaaa
     1321 aaggattaga aatttttaat ggtaaaggtc attgcacatc ttgccactat ggtggtaatt
     1381 tcagcgatgg tcggtttcat aatctcggtg taaacccaac tgacggctct aagccggatg
     1441 aaggcagata tgtgataaca aaaaatccaa aagatatggg tgcatttaaa acacccacca
     1501 taagagatgc atcattaaga gcaccctata tgcacaatgg ctcagaaaaa actttagaag
     1561 aggttgttga gttttataat gttggaggaa ggctggatga tccaaacctt gatccattaa
     1621 ttgttccact tggattaaat gaagacgaga aaaaagcact agttgcattt atgacaagag
     1681 ctttaatcag tacaaaccca gaagtcgcaa atgtaaaacc tgttacgact tcagaattcc
     1741 ctaaatgaag gagattgaaa tgaaaaagtt ttacattttt ctttgtttaa tatttctgat
     1801 agagatcatt ctctccatcg atactcaagc aattccagcc tttgcacgaa aatatggatt
     1861 taactgcaat atgtgccaca ccggatatac taagttaaat gactggg
//
LOCUS       DASWBY010000714         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_326765, whole genome shotgun sequence.
ACCESSION   DASWBY010000714 DASWBY010000000
VERSION     DASWBY010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326765"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VF870_13015"
     CDS             <1..189
                     /locus_tag="VF870_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253160.1"
                     /translation="SAGYYEVLFHPTATERKSAEGKIEFETGYGDIVSGIYLYRIEVI
                     GEGDIPVFTDMKKMMLVK"
     gene            380..1297
                     /locus_tag="VF870_13020"
     CDS             380..1297
                     /locus_tag="VF870_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9253161.1"
                     /translation="MGLLVVGSLGLDTVATPFDKVEDALGGSATYISLAASYFGGPVE
                     LVGVVGSDFPKEYIDIMEEHNVDVEGLQIVEGGKTFKWSGKYHYDLNVRDTLLTELNV
                     FQDFNPIIPDNFKKSKYICLGNIDPVLQAKVLDQMEDPQFIVCDTMNFWIEGKKDELL
                     QLLPRVDVLIINDSEARLLAHEPNLIRAARKIRELGPKILIIKKGEHGALLFTEETVF
                     SAPAYPMETIFDPTGAGDSFAGGFTGYLYKTRDLSPESLKRAVIYGSTMASFCVEKFS
                     TKGLEDLSYLQIQDRYREFMMLSRFDEQE"
BASE COUNT          509 a          246 c          323 g          486 t
ORIGIN      
        1 agtgcaggat attatgaagt gttattccat ccaaccgcga cagaacgaaa aagtgcagaa
       61 ggaaagatag aatttgaaac aggctacgga gatattgtaa gcggaatcta tctttaccgg
      121 atcgaggtaa ttggagaagg cgacattccc gtttttacag atatgaaaaa gatgatgctg
      181 gttaagtaat gcacaattca caatgtacaa tgtagaatga ctgggcaatt aatcaatgaa
      241 acaatcgggc aaagaatcaa aactgctctt cattgtacat tcaaaaatga acatttaaca
      301 ttacaaaaga ttgtgcattc tgcgtgtatt tttctatttt tcttgtatca ttttatcaca
      361 ttataatcag gagtcagttt tgggattatt agtcgtaggt tcactcggtt tggatacagt
      421 tgcaacaccg tttgataaag ttgaagatgc cttaggcgga tcagcaactt acatctcact
      481 tgctgcaagt tattttggcg gtcctgttga gctagttggt gtggtcggca gcgatttccc
      541 aaaagagtat atcgatataa tggaagagca taatgtagat gttgagggtt tgcagattgt
      601 tgaaggcgga aaaactttca agtggtccgg caaatatcat tatgatttga atgtgcgtga
      661 tacactttta accgagttaa atgtttttca ggattttaat cctattattc cagataattt
      721 caaaaaatca aaatacattt gtcttggcaa tattgatcct gtactgcagg caaaagtact
      781 tgatcagatg gaagatccgc aatttattgt ttgcgataca atgaacttct ggatagaagg
      841 aaagaaagat gaactactcc aattacttcc cagagtggat gttttaataa ttaatgacag
      901 tgaagcaaga ttgcttgcac acgaaccaaa tctaattcgt gccgcaagga agataagaga
      961 acttggtcct aaaattctta ttataaaaaa aggcgagcac ggtgcattgc tgtttactga
     1021 agaaactgtt ttttcagcgc cagcatatcc aatggaaaca atttttgatc cgaccggtgc
     1081 gggtgattct tttgccggag gatttacagg ttatttatat aaaacccgag atctttctcc
     1141 agaaagttta aagcgtgcag ttatatatgg aagtacgatg gcttctttct gcgttgaaaa
     1201 attcagcact aagggtttag aggatttatc ctacctgcag atacaggatc gctacagaga
     1261 atttatgatg ctttcaagat ttgatgaaca ggaatagaaa agtcaataaa caaaggttgt
     1321 aatgtcattt cgaacccgac ttgtcgggtg agaaatcttt gacatattta aacagatttc
     1381 tcagtcgttt cactccttcg aaatgaagtt taattaattc agtacgaatt ttaattcaaa
     1441 atgagcaaga ataactatta ctcaataaaa tttttagatg atcatctttc atttctggat
     1501 caaacgaaac ttccgttgaa agaggtttat gttgatactg atgattacga acgtattgct
     1561 attg
//
LOCUS       DASWBY010000715         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_180427, whole genome shotgun sequence.
ACCESSION   DASWBY010000715 DASWBY010000000
VERSION     DASWBY010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180427"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VF870_13025"
     CDS             <1..337
                     /locus_tag="VF870_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9253162.1"
                     /translation="ATTFYKVIEQYEFTSYIKLNLKTGRTHQIRVHLSGLHKPIFGDH
                     TYGGNKIHAGVESPKLKSRIENLLEIMPRQALHAKTLGFIHPHTNKLVRFDSELPEDF
                     KLLLEKLRT"
     gene            539..>1718
                     /gene="cysS"
                     /locus_tag="VF870_13030"
     CDS             539..>1718
                     /gene="cysS"
                     /locus_tag="VF870_13030"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX9253163.1"
                     /translation="MLKLYNTLTKKLEEFIPIDPNEVKFYVCGPTVYDYFHIGNARSF
                     IMADIVRKYLEYKGYKVKFVMNLTDVDDKIIKKANEENRKPNEVTNDYINAFFDDLSK
                     LKIKKANVYPKATEHMNDIIGMIKNLEDKKFAYNIDGNVFYDVKHFDQYGKLSGKNIE
                     DLESGARIEINEEKSNPLDFALWKKAKEGEPYWESPWGNGRPGWHIECSAMSCKHLGE
                     TFDIHAGGNDLIFPHHENEIAQSEAATGKPFVKYWLHFGFLNINEEKMSKSLGNFFTA
                     RDVLKRYSAEAIRMFFAQAYYRGPLNFTDDLLTGAEKGLEKISNLVEKISDELKKNNS
                     GTKPEFDFPSFYKRFEEAMDEDLNTSQAVAVIFDFAREVNRVFVTNENVDVSFYNDVK
                     T"
BASE COUNT          626 a          262 c          314 g          516 t
ORIGIN      
        1 tgcaaccact ttttataaag tgattgagca gtacgagttt acctcttata taaaacttaa
       61 tttgaaaaca ggaagaacac atcaaatacg agtgcatctt tccgggttgc acaaaccaat
      121 ttttggtgat catacttatg gcggaaataa aattcacgcc ggagttgaat caccaaaact
      181 taaaagcaga attgaaaatc ttttagaaat aatgccaagg caagctttac acgcaaaaac
      241 attgggcttc attcatccgc atacaaacaa acttgtacga tttgattctg aactgcctga
      301 agattttaag ttgcttttag aaaaactacg cacataagat cgtcattgcg agcttagcaa
      361 tcttataata cttaatgtct gtcaacctga actcgttaca ggttcttttt atttgaacaa
      421 tgtcatccaa agattccgaa acaagttcgg aatgactgtc aaaggataat tgcaatgaca
      481 tacattaatc attttcttta tatttgtaac aacaattatc aatcaaaaaa caataactat
      541 gctaaaactc tacaacactc ttacaaaaaa attagaagaa tttattccaa ttgatccgaa
      601 tgaagtaaaa ttttacgtat gcggaccaac ggtgtatgat tactttcata taggaaatgc
      661 acgctctttt attatggcgg atatcgtgcg aaagtatctg gagtataaag gttataaagt
      721 aaagtttgtt atgaatctta ctgatgttga tgataagatc attaagaagg caaatgaaga
      781 aaatcgaaaa ccgaatgaag ttacaaacga ttacataaat gctttttttg atgatttatc
      841 aaaacttaaa atcaaaaaag caaatgtgta tccaaaagct accgagcata tgaatgatat
      901 aatcggaatg ataaaaaatc ttgaagataa aaaatttgct tacaacattg atggaaatgt
      961 tttttatgat gtaaaacatt ttgatcaata tggaaaacta agcggaaaaa atattgaaga
     1021 tcttgaatcg ggtgcacgaa tagaaattaa tgaagagaaa tctaatccat tggattttgc
     1081 tttgtggaaa aaagcaaaag aaggcgagcc ttactgggaa agtccttggg gcaatggtcg
     1141 tcctggctgg catattgaat gctctgcaat gagttgcaaa catcttggcg aaacatttga
     1201 tattcacgct ggtggaaatg atttgatttt cccgcatcac gaaaatgaaa ttgcacaaag
     1261 tgaagctgca acaggaaaac catttgttaa atactggctt catttcggtt ttcttaatat
     1321 caatgaagaa aagatgtcta aatcgcttgg caactttttt acagcacgag atgtgttaaa
     1381 aagatattcc gccgaagcaa taagaatgtt ttttgcgcag gcttattatc gcggaccgtt
     1441 aaactttact gatgatcttt taactggagc agaaaaagga ctggaaaaaa tctcaaatct
     1501 tgtagaaaaa attagtgatg agttgaagaa gaataattcc ggcactaaac ctgaattcga
     1561 tttcccgtcg ttctacaaaa gatttgaaga agcgatggat gaagatctta atacctcaca
     1621 agctgttgcc gttatatttg attttgcaag agaggtaaat cgtgtttttg tgactaatga
     1681 aaatgttgat gttagttttt ataatgatgt aaaaacgt
//
LOCUS       DASWBY010000716         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_30909, whole genome shotgun sequence.
ACCESSION   DASWBY010000716 DASWBY010000000
VERSION     DASWBY010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30909"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VF870_13035"
     CDS             <1..1250
                     /locus_tag="VF870_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9253164.1"
                     /translation="VLSNGKNINPEEIENKILSFSEVITEIGVFEKQDMLHAVIYPDY
                     QKAKQLSIENVEEMIKWQVIDKYNQTVTPYKKVMKFSLVKEPLPRTRLLKLKRFLLPQ
                     LEQISKEKLESISEPTFQEYTLLKEFLQQQKEPTVHPSDHLEIDLGLDSLDKVNLGVY
                     LESNFGIKYTEAELVGFTNVLKLAENIRDKKTKLSVEAIDWGKIFKEQLDLDLPKSWF
                     THNIMKNGAKVFLKLYFRLKGEGLENLPEGPFILAPNHQSFFDGLFVAVFLKNKLMKQ
                     TYFYAKEKHVKNSFLKLLAAKNNVIVMDLSDLKTSLQKMAEVLKRGKNIIIFPEGTRT
                     QTGEIGDFKKTFAILSRELNVPIVPVAISGAYDALPRGTHFPKPWKKINVKFLKPVLP
                     ENLSYDSLKDIVQSKVAEQLIRR"
     gene            1394..2974
                     /locus_tag="VF870_13040"
     CDS             1394..2974
                     /locus_tag="VF870_13040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9253165.1"
                     /translation="MKKIGLILILIIILPIAFLLVNELGKLNENEQVLEQIYNDQLQA
                     ILFSVNQYSEDVVSASAKSIIDDIDSINNSSRRAETFNNLLNQNKLVTFIFTIDSLYS
                     QNITFYFKRNSGVTNLDSLEQYFKDVIANNADKINKLYSYRDIGYNKIEPIVLPNSNI
                     QSLIVFTGLNNQSKKIYGVVINTQEFITNVLSSRLQQVAKNEFVISVINPKTGFQYNS
                     TKDFERRPLQAQKALWIFPDYNLGISLVGQSIEDLVTERAMNNLILIGVLLIVLITAV
                     WFVYSAVKKELELAQAKADFVSNVSHELRTPLALISMFAETLEMDRVKTEEKKKEYYS
                     IISHESNRLGRIVNTILNFSKMEAGKRKFDFKEEDLNEVIIQVYQSYSYHLYNKGFDF
                     EYEPGIDIPKIVIDREAVSEAIVNLLDNAVKYSGETKFIKMVIGNENDSVFIEVQDKG
                     IGISEENQQKVFDKFYRVSSGLVYTTKGTGLGLSLVKQIMEAHKGKIILKSKIGEGSS
                     FKLVFPKNSQLINGDKNA"
BASE COUNT         1115 a          419 c          529 g          971 t
ORIGIN      
        1 ttgttttatc aaatggaaaa aatattaatc ctgaagagat tgaaaataag atcttaagct
       61 tttcagaagt aattacagag attggagttt ttgaaaagca ggatatgctt cacgcagtta
      121 tttatcctga ttaccagaaa gcaaagcagc tctctattga aaatgttgaa gagatgataa
      181 agtggcaggt gattgataaa tacaatcaaa cagttactcc ttataaaaaa gttatgaagt
      241 ttagtttagt taaggaaccg ttaccacgaa caagattatt aaagcttaaa agatttttat
      301 taccgcagct tgaacagata tcaaaagaaa aattagaaag tatttcagaa ccaacttttc
      361 aggaatacac tttgcttaag gaatttctgc agcagcagaa agaacctaca gttcatccat
      421 ctgatcatct tgaaattgat ttgggacttg attcactgga taaagttaat cttggggttt
      481 atctggaatc aaattttgga attaaatata ctgaagctga gcttgttggc tttacaaatg
      541 ttcttaagct tgcagaaaat atacgtgata aaaaaacaaa gttgagtgtt gaagcaattg
      601 actggggaaa gatattcaaa gaacagcttg atcttgattt accaaaaagc tggtttactc
      661 ataacatcat gaaaaatggc gctaaagttt ttcttaaact ttattttaga ttgaaaggtg
      721 aaggattaga aaatttgcct gaaggtccat ttatccttgc accaaatcat caaagctttt
      781 ttgatggatt atttgttgct gtatttctaa aaaataaatt aatgaaacaa acatattttt
      841 atgcaaaaga aaaacatgta aaaaatagtt tcttaaaatt acttgcagca aaaaataatg
      901 taatcgttat ggatttaagt gacttaaaaa catctttaca aaaaatggca gaagttttaa
      961 aacgcgggaa aaatattata atctttcctg aaggtacacg tacccaaaca ggtgagattg
     1021 gtgatttcaa aaaaacattt gcaattctta gtcgggaatt aaacgtacca attgtaccgg
     1081 ttgcgataag cggagcttat gacgcacttc cacgcgggac acatttccca aagccttgga
     1141 agaaaattaa tgttaaattt ttaaagccag ttttacctga aaatctttct tatgattctt
     1201 taaaagatat tgtacaaagt aaagttgctg aacagttaat taggagataa actttttgtc
     1261 attcccgcgg aggcggaaat ctttatcgat attattaaga aatctgaact tatcatcaat
     1321 ccttctctca aataagaagg tatagtgcag gttctcatta ataatatatt aatgtaattg
     1381 aatttaatac cccatgaaaa aaataggttt aatactaata ctcatcataa ttctacctat
     1441 tgcatttcta cttgttaatg aacttggcaa gttgaatgaa aacgaacaag ttcttgaaca
     1501 aatctataac gatcagcttc aggcaattct attttcggtc aatcaatatt cagaagatgt
     1561 tgtaagcgca agcgctaaaa gcataattga tgatattgat tccattaaca attcttcaag
     1621 aagagcagag acctttaata atctgttgaa ccaaaataaa ttagtgacat ttatttttac
     1681 aattgatagt ctgtattctc aaaacataac tttttatttt aagagaaata gcggcgtaac
     1741 aaatcttgat tcacttgaac agtactttaa agatgtgatt gcaaataatg ccgataaaat
     1801 aaacaaactt tacagttatc gggatatagg atataataaa attgaaccga ttgttcttcc
     1861 taactcaaac attcaatcac tgattgtttt tactgggttg aataatcaat caaagaaaat
     1921 ttacggcgtt gttataaata cgcaggaatt tattacaaat gttttatcat cgcgcttgca
     1981 acaagttgct aaaaatgaat ttgtaatttc tgttataaat cccaaaacag gttttcagta
     2041 caattctacg aaagatttcg aaagacgacc attacaagct caaaaagctt tatggatttt
     2101 tcccgattat aatcttggga tttctttagt cggacaatcg attgaagatt tagtaactga
     2161 acgcgcaatg aataacctga tcctgattgg tgttttgtta atcgttttaa ttaccgctgt
     2221 gtggtttgtt tatagtgctg ttaaaaaaga attggaactt gcacaggcaa aagctgattt
     2281 tgtatcaaat gtttcgcacg aacttcggac tccgcttgcg ttaataagca tgtttgcaga
     2341 aactcttgag atggatcgcg taaaaacaga ggagaagaaa aaagaatatt attctataat
     2401 aagtcacgaa tcaaatcgtt taggaagaat agtaaacaca attttaaatt tttctaaaat
     2461 ggaagccggt aaacgtaaat ttgattttaa agaagaagat ttaaatgaag taattattca
     2521 ggtctatcag agttacagtt atcatcttta caacaaggga tttgattttg aatacgaacc
     2581 tggaattgat attcctaaaa tagttatcga tcgtgaggct gtttcggaag caatagtaaa
     2641 tcttttagac aatgccgtaa agtatagtgg cgaaacaaag tttattaaaa tggtaatcgg
     2701 taatgaaaat gattctgtgt ttatagaagt gcaggataaa ggaattggaa tttctgaaga
     2761 gaatcaacaa aaagtttttg ataaatttta ccgtgtttct tcaggtcttg tatatactac
     2821 aaaaggtacc gggctcggac tttcacttgt aaagcaaatt atggaagcac ataaaggaaa
     2881 aattattctt aaaagtaaaa tcggtgaagg aagtagtttt aagttagtgt ttcctaaaaa
     2941 ttctcagtta attaatggag ataaaaatgc ctaaaatttt agtcgttgat gatgaacaaa
     3001 atatgcgaac agggttaaaa gataatcttg aatt
//
LOCUS       DASWBY010000717         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_398631, whole genome shotgun sequence.
ACCESSION   DASWBY010000717 DASWBY010000000
VERSION     DASWBY010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398631"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VF870_13045"
     CDS             <1..648
                     /locus_tag="VF870_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX9253166.1"
                     /translation="IEIWNHMSEWVENLTEENKYRSFLHPLRTIIAPPKETLKVWDEL
                     SQKRKVIGIGGIDAHAHKYNLVGFLEVEIFPYKVLFKSIRTHILIEDVIKKGKSAKDI
                     EKAKWQIYDALKDGRCFVANDYVAESKGFRFYAESNGIKYQMGDTVPDHKNVSLKVFL
                     PGISAEIKLLRNGHIIETNNGMDAEFVVNKKGVYRVEVFNEDRAWIYSNHIRVNI"
     gene            782..1039
                     /locus_tag="VF870_13050"
     CDS             782..1039
                     /locus_tag="VF870_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX9253167.1"
                     /translation="MIPAQKQPKVIVFSTPTCSFCNQAKRYFKEKNIRFTDVDVSKDQ
                     SAARDMMLRTGHKGVPVILINNKPIIGFDRNKINQMLSIKN"
     gene            1050..1322
                     /locus_tag="VF870_13055"
     CDS             1050..1322
                     /locus_tag="VF870_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9253168.1"
                     /translation="MKIKPLDDRILILPMPAEEKTSSGLIIPDSAKEKPRMGTVIAVG
                     TDEDLQEKIKEGNKVLFAKYGGDEIEIDGKEYRIIQRSDILAVVED"
     gene            1349..1693
                     /locus_tag="VF870_13060"
     CDS             1349..1693
                     /locus_tag="VF870_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9253169.1"
                     /translation="MLKTNLTHVLSEKDHAELLKNNENVMICCGRMGPMCIPVYEVME
                     ELEGEYKKIKFADMEFDSPDAKVIRSLPEARSFQGLPFTIYYKNGKVVKATSSIQSRD
                     QVTAILDEHFGK"
     gene            1766..>2198
                     /locus_tag="VF870_13065"
     CDS             1766..>2198
                     /locus_tag="VF870_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9253170.1"
                     /translation="MNNHFDVIVIGAGAAGLTTGIYLSRAKVKTLILNEGTVGGQMVL
                     THEIANYPGVENISGYELARTMKTQAQKFGCTIKSNLKITSMDFKDDFKRVVVNSKEI
                     FTSEAVIIATGGRSRMIGAIGEDKFKGKGISYCATCDGDFFQ"
BASE COUNT          823 a          305 c          411 g          659 t
ORIGIN      
        1 attgaaatct ggaatcacat gtctgagtgg gttgaaaatc ttactgaaga aaacaagtat
       61 agatcatttc ttcatccttt aagaacaatc attgccccgc ctaaagaaac attaaaagtc
      121 tgggatgaac ttagtcagaa aagaaaagta attggtattg gcggaataga tgctcatgca
      181 cataaatata atctggtcgg atttttagaa gtagaaattt tcccttacaa ggttttgttc
      241 aaatctataa ggactcacat tcttattgaa gatgttataa aaaaaggaaa gagtgcaaaa
      301 gatatagaga aagctaaatg gcaaatctat gatgcactta aagacggaag atgttttgta
      361 gcaaatgatt atgttgctga atcaaaaggg tttagattct atgctgaatc aaatggtatt
      421 aaatatcaaa tgggtgacac tgttcctgat cataaaaatg tttcacttaa ggttttttta
      481 cccggaatta gtgctgagat aaaactactt cggaacggac acattataga aactaataat
      541 ggtatggatg cagagtttgt tgtaaataaa aaaggtgttt accgggttga agtttttaat
      601 gaagatcgtg cctggattta ttccaatcat ataagggtga atatttaatt aaacaacttt
      661 aagctttttc atcttacaat ctgcttgtta actgaccatt cctttcaatt caccccacaa
      721 tctgtgaatc tttattcttg ataatgaatc ataccatcac aaaaaattat tattaattaa
      781 tatgatacca gcacaaaaac aaccaaaggt cattgtattt tcaacaccca cctgctcttt
      841 ttgtaatcag gcaaaaagat actttaaaga gaaaaatata agatttactg atgtggatgt
      901 ttcaaaagat caatcagctg caagagatat gatgcttaga acaggtcata agggtgttcc
      961 ggtgattctg ataaataata aaccaataat agggtttgat agaaataaaa taaatcaaat
     1021 gttaagtatt aaaaattgat ggagttataa tgaaaattaa accgttagac gatcgcatat
     1081 taatattacc aatgcctgct gaagaaaaaa catcatcagg tttaatcatt cctgattccg
     1141 caaaagaaaa accgcgtatg ggaaccgtaa ttgctgtcgg tacagatgaa gatctgcaag
     1201 aaaaaattaa agaaggaaat aaagttttat tcgctaaata tggcggagat gaaatagaaa
     1261 ttgacggcaa ggaatataga ataatacaac gctctgatat tcttgcagtt gttgaagatt
     1321 aaataattaa aaatcagaag gataaaaaat gttaaaaaca aatcttactc acgtactttc
     1381 agaaaaagat catgctgaat tattaaagaa taacgaaaac gttatgatat gctgcggtag
     1441 aatgggacca atgtgtattc ctgtttatga agtcatggaa gaacttgaag gcgaatacaa
     1501 aaagattaag tttgctgata tggagtttga ttctcccgat gctaaagtta taagaagtct
     1561 tccggaagca agaagttttc aagggttacc tttcaccatt tattataaaa atggaaaagt
     1621 ggtaaaagct acaagcagca ttcaatcaag agatcaggta actgcgattc ttgatgaaca
     1681 ttttggtaaa taatttactt acataattaa aagatgatat ccgataaaag gatatcatct
     1741 ttaacaattt aaattttaga aatttatgaa taatcatttc gatgtaatag ttattggtgc
     1801 cggagctgcc ggacttacaa ccgggattta tctttcacgt gctaaagtta aaactttaat
     1861 tttaaatgaa ggcacagtcg gcggacaaat ggttcttact cacgaaattg caaactatcc
     1921 cggtgtagaa aatataagcg gatatgaact tgcaagaact atgaaaacgc aggcacaaaa
     1981 atttggatgc accatcaaat cgaatttaaa aatcacttct atggatttta aagatgattt
     2041 caaaagagta gttgtaaata gtaaagagat ttttacatct gaagctgtta taattgctac
     2101 cggcggaaga tcaagaatga ttggtgcgat tggtgaggat aaatttaaag gtaaaggtat
     2161 ttcgtattgt gcaacctgtg acggcgattt ctttcagg
//
LOCUS       DASWBY010000718         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_216850, whole genome shotgun sequence.
ACCESSION   DASWBY010000718 DASWBY010000000
VERSION     DASWBY010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216850"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..158)
                     /locus_tag="VF870_13070"
     tRNA            complement(86..158)
                     /locus_tag="VF870_13070"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Phe,seq:gaa)
     gene            complement(228..>1722)
                     /gene="glmS"
                     /locus_tag="VF870_13075"
     CDS             complement(228..>1722)
                     /gene="glmS"
                     /locus_tag="VF870_13075"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX9253171.1"
                     /translation="MGFGYKFVSETDSEVLVHLIDSFTKRGHNLTKSVQLALHEVDGT
                     YGLAVISSKEPDKIIAARRGSPLVIGIGEDENFLASDISAIIAHTKQIIYLEDGEIAI
                     IKKDEFTAKTIYDNDIEKEVHEISMTLDEIDKGGYPHFMLKEIMEQPESLRNSIRGRI
                     LHTEGDVRLGGLADVVEKIANSKRIIITACGTSWHAGLVGEYMLEQFCRIPTEVEYAS
                     EFRYRNPIIEKDDAIFFISQSGETADTLAAVREAKMKGALVLGICNAVGSSIARETNA
                     GVYTHAGPEIGVASTKAFTSQLTVLALITILIARRKSLSRVDGKQIADELLSIPDKVE
                     KILQLNDEIEKIAEEFKDATNFLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMK
                     HGPIALIDENMPVVFIAPKDSTYEKIVSNIQEVKARGGRTIAIASEDDLEIDQLVDYS
                     IKIPHTIRMLMPILTVIPLQLLAYHIAVKKGLNVDQPRNLAKSVTVE"
BASE COUNT          521 a          366 c          278 g          557 t
ORIGIN      
        1 gttcttccct accccactaa gtagaaatat tgcgaataca agctgaaaaa caaaaaaccc
       61 acttcggtaa gcgggttaaa gggtgtgggc agggacggaa ttgaaccgcc gacacaagga
      121 ttttcagtcc tttgctctac cgactgagct acctgccctt caaaatttgg agtgtaaaaa
      181 tatggaattt ctgcaaaatc acaaacagaa ttgaaaatta aatttatcta ttctacagtt
      241 acacttttag caagatttct tggctgatcc acatttaatc cttttttaac agcaatatgg
      301 taagcgagta attgcaaggg aattactgta agaattggca ttaacattct tatagtatga
      361 ggaattttta ttgaataatc tacaagctga tcaatttcaa gatcatcttc acttgcgata
      421 gcgatagttc gtccaccacg tgctttaacc tcctgtatgt tgcttacgat cttttcataa
      481 gtagagtctt ttggtgctat aaataccaca ggcatatttt catctattaa agcaatcggt
      541 ccgtgtttca tctcagcagc aggatatcct tcagcgtgga tatatgatat ttctttaagt
      601 tttagtgcac cttccagcgc aacaggaaaa ttatatccac gtccaagata taagaaatta
      661 gtagcatctt taaattcctc cgctatcttt tcgatttcat cattaagctg taaaatcttt
      721 tcaactttat caggaattga taataattca tctgctattt gtttcccatc aacacgactt
      781 aaactttttc ttcttgcaat tagaattgtg attaaggcta aaaccgttaa ctgagaagta
      841 aatgcttttg ttgaagctac accaatttca ggtccggcat gtgtataaac ccctgcattt
      901 gtctcccgtg caattgaact accaacagca ttacaaatac caagtacaag tgcgcctttc
      961 atttttgctt cgcgaacagc ggcaagtgta tcagcagttt caccactttg tgatataaaa
     1021 aatatcgcat catctttttc aattatcgga tttctatatc taaattccga ggcatactca
     1081 acttcagtag gaattctgca aaactgttcc aacatatact caccaaccaa accggcgtgc
     1141 caggatgttc cgcaagcagt aatgataatc cttttagaat ttgcaatctt ctcaacaacg
     1201 tcggctaacc cgcctaagcg cacatcacct tcagtatgta gtattcttcc tctgattgag
     1261 ttccttaatg attctggctg ctccatgatt tctttgagca taaaatgagg ataaccgccc
     1321 ttatcaatct catccagagt cattgatatt tcgtgaactt ctttttctat atcattatcg
     1381 taaatagtct ttgcggtaaa ttcatctttt ttaataattg cgatttcacc atcttcaagg
     1441 tagataatct gtttggtatg agcaatgatc gccgatatat cagaggctag aaagttttca
     1501 tcttcaccaa ttccaataac aagaggcgat cctcttctgg ctgcgataat cttatccggt
     1561 tcttttgagg aaataacagc caatccataa gtcccatcaa cttcgtgtaa cgcaagctga
     1621 acagactttg ttaaattatg tcctcgttta gtaaaactat caattagatg aacaagaact
     1681 tcagaatcag tttcgcttac aaatttgtac ccaaaaccca tc
//
LOCUS       DASWBY010000719         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_65947, whole genome shotgun sequence.
ACCESSION   DASWBY010000719 DASWBY010000000
VERSION     DASWBY010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65947"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VF870_13080"
     CDS             <1..283
                     /locus_tag="VF870_13080"
                     /inference="COORDINATES: protein motif:HMM:NF016216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HEX9253172.1"
                     /translation="EEGMIVIQRLFLMDSDALRTGLNLVRSFKPDAIEILPGFVPASV
                     IKRIIDETGLPILAGGLIRTQEDVNQAIERGISAVSTSRQELWDDIMIK"
     gene            783..1517
                     /locus_tag="VF870_13085"
     CDS             783..1517
                     /locus_tag="VF870_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX9253173.1"
                     /translation="MTNLFGEFFGTMILLTFGCGVVANVLLKDSKGNGGGWIVITTGW
                     ALAVLLGIFGAVSTGAPQADLNPAVTLFKMFLGVYTVPQAIATMFAEMAGGIVGGMIC
                     WLVYLPHWEATEDKGLKLAVFATGPAIRNSMANILCEIIATAFLLIGIFTIFSKGVTG
                     TAGYSTGVGPYMVGMLIWALGLSLGGPTGYALNPARDLGPRIAHAIAPIAGKGSSDWG
                     YAWIPVVGPMLGACLAFAVCRAAGIV"
     gene            1627..>2398
                     /locus_tag="VF870_13090"
     CDS             1627..>2398
                     /locus_tag="VF870_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX9253174.1"
                     /translation="MTKKYVMALDAGTTSNRAIIFDQDSNIVAVAQKEFTQIFPKAGW
                     VEHDADEIWSTQIDVMKQVVQQAGINPGDISAIGITNQRETAVVWDKTTGRPVYNAIV
                     WQSRQTMDICNELKAKGYEAEFKQKTGLVVDAYFSGTKVKWILDNVEGARAKAEKCEL
                     LFGTIDTWLIWKLTGGKVHVTDYSNASRTLMYNIRELKWDPKLLEYLTVPACLLPEVK
                     SSSEVYGQTDVAIFGAAVPISGAAGDQQAALFGQTCFKP"
BASE COUNT          746 a          336 c          528 g          788 t
ORIGIN      
        1 ggaagaaggt atgattgtta tacaaaggct ctttttaatg gattcagatg cattacgtac
       61 cggacttaat ttagtgcgta gttttaaacc agatgccatt gagatattgc caggttttgt
      121 tcctgccagt gttataaaac gaattattga tgaaacaggt ttacctattt tagcgggtgg
      181 cttaatacgt acccaggaag atgtaaatca ggccattgaa agaggaattt ctgccgtaag
      241 tactagtcga caagaattat gggatgatat tatgataaag tgagattatc tttttgatag
      301 taaattcgtg gcttaatggg ctttaataaa taaatagtgg taaaataata aaaaaatatg
      361 ataaatcagt aaattcaagc aggacttttt aatggtaata tcgaaactaa agtaagtata
      421 gaaaaagagc attgctaaca tagggaatat gcattttgcc aaggacaata atttagtgaa
      481 aaaagttttt agaaaaaaac gaaattttca ataaattaag caggagatta taaacggatg
      541 gcgaaatata gataatatag aataaattag tgattatgga aaataaaaga ataattttgg
      601 gctagagata aagagaagcc catttatgcc tttgataatt taatatcaaa ggtgtagatg
      661 ggcttctctt ttttatctaa ataaataggg ataaaggacg tgatgaaggg aaaataagtg
      721 agtgaatttt agtggtttcc ggcaataata atttttattt aaattaagga ggaattggaa
      781 ttatgacaaa tttatttggt gaattttttg gtacgatgat ccttttgact tttggttgtg
      841 gtgttgttgc taacgtcctg ttaaaagatt ctaaaggtaa tggtggcggt tggatcgtta
      901 ttactacagg ttgggcatta gcagtgttac ttggtatatt tggtgctgtt tctactggtg
      961 ctcctcaagc ggatcttaac ccagcagtta ctctatttaa aatgttttta ggtgtataca
     1021 cagtgccaca agcaatcgct acgatgtttg ctgaaatggc cggtggtatt gttggtggta
     1081 tgatttgttg gttagtatac ttaccacatt gggaagctac tgaagacaaa ggtttgaaat
     1141 tagcagtatt cgcaactggt cctgccattc gtaattcaat ggctaatata ctttgcgaaa
     1201 ttattgcgac tgccttctta ttaattggta tctttactat tttcagtaaa ggtgtaactg
     1261 gtactgctgg ttattcaact ggcgttggtc cttatatggt tggtatgtta atttgggctc
     1321 ttggcctaag tttaggtggc cctactggtt atgcacttaa ccctgctcgt gaccttggac
     1381 ctcgtattgc tcatgcaatt gcaccaatcg ctggtaaagg tagttccgat tggggttatg
     1441 cttggatccc tgtagttggt ccaatgcttg gcgcttgtct tgctttcgca gtttgtagag
     1501 ctgctggtat tgtttaatat cagtcttagc aagtaacgag agtttaaatt agatataaag
     1561 ttagttgttg ttttaggatt aaagagaaaa agaaataaac ctaaattaaa aggagtggct
     1621 tttaaaatga ctaaaaaata tgtaatggca cttgatgcag gtactactag taatcgggca
     1681 atcatttttg atcaagattc caatattgtt gctgtagctc aaaaagaatt tactcagatt
     1741 ttccctaaag ctggttgggt tgaacatgat gctgatgaaa tttggagtac ccaaattgac
     1801 gtaatgaagc aagttgtaca acaagctgga ataaatcctg gtgatatttc tgcaattggt
     1861 atcacaaatc aacgcgaaac ggcagtggtt tgggacaaaa caactggcag accagtttat
     1921 aatgcaattg tttggcaatc acgtcagaca atggatattt gtaatgaatt aaaagcaaaa
     1981 ggttatgaag cagagtttaa acaaaaaact ggcttagttg ttgatgctta tttctcaggt
     2041 acaaaagtaa aatggatttt agataatgta gaaggcgctc gcgctaaagc tgaaaaatgc
     2101 gaactattat tcggtaccat tgatacttgg ttgatttgga aattgaccgg cggaaaagtt
     2161 catgttactg attattctaa tgcttcccgt actttgatgt acaacattcg tgaattgaaa
     2221 tgggatccta aacttcttga atatctaact gtaccagcat gcttgctgcc agaagttaaa
     2281 tcttccagcg aagtatatgg tcaaactgat gttgctatct ttggtgctgc tgtgccaatc
     2341 tctggtgctg ctggtgacca acaagctgct ttatttggtc aaacttgctt taaacctg
//
LOCUS       DASWBY010000720         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_398655, whole genome shotgun sequence.
ACCESSION   DASWBY010000720 DASWBY010000000
VERSION     DASWBY010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398655"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VF870_13095"
     CDS             <1..1293
                     /locus_tag="VF870_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253175.1"
                     /translation="IQLTVLASPQHENVTINFTNMSPHINQNLYLRIVDKSTLKESDR
                     TFVLISSASFSVTLPAVDVGGSYFVDFFADVNSNAKYDAPPTDHAWRINLNNAAGNDT
                     LNFTHNTSFTDIKWQNILTVNFSGMTPHIGQMLEIKLENSTLEKEIGRIKIPAIASAS
                     FPVQIIGLIEPGDYSIEFYVDLNGNGIYDAPPTDHAWKIGFNYTGGNAEVNSAHNATF
                     TDIQWKYLLTVNFAAMTPHVGQQFELRLVKVENQEEAGRVTLPAILVPNFTVYVPGFD
                     LNKDYNIDFYADLNGNGSYNVPPVDHAWRLNFNSSTGDFLSNFSHNANFTDIQWPGAT
                     DINDETSLVDHFSLEQNYPNPFNPSTSIQFAIANRQFVTLKVYDVIGNEVATLVNEEK
                     AAGSYKIDFNATSLSSGVYFYKLQTNSFVETKKMTLLK"
     gene            1468..2232
                     /locus_tag="VF870_13100"
     CDS             1468..2232
                     /locus_tag="VF870_13100"
                     /inference="COORDINATES: protein motif:HMM:NF045274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253176.1"
                     /translation="MKRTFYQLMFILFTPLVICQTSNEFIAKGDDYNKKFDLKNAAKY
                     YEEAYKISSDSYYALLKITKIYNDLGEDYNENKDEDGAENAFNIALKYAKEFSSKFPD
                     SAKVYTLLAMSYGNLALFEGGNSKLKLAYLIRDNAVKAIEKDSSDYLPYIILSIYNRQ
                     IASLNWFERAFANTFFGKVPDGSLKESERLMLKSLKIEPGIVTAMYQLSLTYKEMGNE
                     QKEIEWLKKVLDAPITDFRDKYAKRKSKDRLSELNN"
BASE COUNT          843 a          386 c          436 g          755 t
ORIGIN      
        1 atacaattaa ctgtgcttgc ttcaccccag cacgaaaatg ttacaattaa ctttaccaat
       61 atgtccccgc atattaatca gaatctttac ttacggattg ttgacaaaag caccttaaaa
      121 gaaagtgaca gaacttttgt actaatctca tcagcaagtt ttagtgttac tttacctgct
      181 gttgatgttg gagggagtta ctttgtggat ttcttcgcag acgtcaattc taatgcaaaa
      241 tacgatgcac cacctacgga tcacgcctgg agaattaatt taaataatgc agcagggaat
      301 gatacactta attttacgca taataccagc tttactgata ttaaatggca aaacattctt
      361 acagttaatt tttcaggaat gacgcctcat attggacaga tgctcgaaat taaactcgag
      421 aacagcaccc ttgaaaaaga gattgggcgt attaagatac cagcaattgc ttctgcgtca
      481 tttccggttc agattattgg attgatagag ccaggtgatt atagcataga gttttatgtt
      541 gatcttaatg gaaatggaat ttacgatgcg ccaccaacag accatgcatg gaaaataggt
      601 tttaattata ccggcggaaa tgcagaagtt aattctgcac acaatgccac atttacagat
      661 atccaatgga aatatttatt aactgttaac tttgcagcaa tgactccgca tgttggacag
      721 caatttgaat tacgcttggt taaagttgaa aaccaggaag aagcaggcag agttacctta
      781 ccggctatac tggttccgaa ctttacagta tatgtaccag ggtttgatct aaataaggat
      841 tacaatatag atttttatgc ggatctgaat ggaaatggat catataatgt tccaccagtt
      901 gatcatgcat ggagattaaa ttttaattct agcacaggcg atttcttatc taatttttca
      961 cacaatgcaa actttacaga tattcaatgg ccgggtgcta cagatataaa tgatgaaacc
     1021 tcattagtgg atcatttttc attggagcag aattatccta atccatttaa ccctagcacc
     1081 agtatacagt ttgcaatagc caatagacaa tttgttacat taaaagtata cgatgttatc
     1141 ggaaatgaag ttgccacact tgttaatgag gaaaaagccg cgggaagtta taaaatagat
     1201 tttaatgcga cttcactttc aagcggcgta tatttttaca aactgcaaac aaatagtttt
     1261 gttgagacta aaaaaatgac gctattaaaa tagcctacta tcggaaatta atttataagg
     1321 ccctgatatt ccaagtatca gggacttaat atttatggta ttgaactgat tacttaaatt
     1381 tgattatgat taagccttca atttttagat gtcttttgtc aagttttatc tgcattatta
     1441 tttgaatagt aaaaaatgag gcaacgagtg aaacgaactt tttatcaatt aatgtttatt
     1501 ttgtttaccc ctttagttat ctgccaaacc tctaatgagt ttatagccaa aggcgatgat
     1561 tacaataaaa aatttgactt aaaaaatgcg gcgaaatatt acgaagaggc ttataaaatt
     1621 agttctgata gttattatgc actgctaaaa ataactaaaa tatataacga ccttggtgag
     1681 gattataatg agaataagga tgaagacggg gcggaaaatg cttttaatat tgcactaaaa
     1741 tatgcaaagg aattcagttc aaaatttccg gatagtgcta aagtttatac gcttctggca
     1801 atgtcatatg gtaatcttgc attgtttgaa ggaggaaata gtaaattaaa acttgcttat
     1861 ttaattagag ataatgctgt taaagcgatt gagaaagatt cctcagatta cctaccatac
     1921 ataattttaa gtatttataa ccgccaaatt gcaagtctaa attggtttga acgtgcattt
     1981 gcaaatacat tttttggaaa agtaccagat ggaagcctta aagaatcaga acgactaatg
     2041 ttaaaatctt taaagattga gcctggaata gtgactgcta tgtatcaact ttctcttaca
     2101 tataaagaga tgggcaatga gcaaaaagaa attgagtggc ttaaaaaagt acttgatgca
     2161 ccaataactg atttcagaga taaatatgca aaaaggaaat cgaaagacag attatcagaa
     2221 ttaaataatt aaataacaca gtaaattaac ccgattaaca tttttatttc gttctggtta
     2281 taacagagtt tagttgcggt caagatctat atcttcccag gatgccgggt cgtctaaaga
     2341 ttcaagatgt gtaaaaacag taacacccgg aagcgaatgt cttatactgg actcaatatt
     2401 ttccagcaat ctatgacttc
//
LOCUS       DASWBY010000721         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_180661, whole genome shotgun sequence.
ACCESSION   DASWBY010000721 DASWBY010000000
VERSION     DASWBY010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180661"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF870_13105"
     CDS             <1..213
                     /locus_tag="VF870_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253177.1"
                     /translation="VSVGKTFATAGNYWLIGEASNGTTTVRDSVWMVVKKEVETLTRP
                     SNTRAGINVQDACHVLKKVDTFYWLI"
     gene            complement(432..1274)
                     /locus_tag="VF870_13110"
     CDS             complement(432..1274)
                     /locus_tag="VF870_13110"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX9253178.1"
                     /translation="MSELRKAVQVNQSCAYFGISRSGYYSSLQSVEKEMLRESVIIEL
                     VLNIRKRMPRVGAKKLYYMLKDDIGEIGKIGRDKFIDILRSNELLVERKKSFTRTTDS
                     YHRFYKYKNLLYNNEIETPNACWVSDITYLRTEKGFVYLFLITDYFSRKIVGWSLSDS
                     LSIEGGIEALKMALRQRDKDKPLIHHSDRGIQYCSNEYVKILKKNTIQISMTEENHCY
                     ENSKAERVNGILKDEFLLDETFKDYEATHKSVKEAIRTYNELRPHWALKLKTPQEVHI
                     QKAA"
     gene            complement(1244..1624)
                     /locus_tag="VF870_13115"
     CDS             complement(1244..1624)
                     /locus_tag="VF870_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9253179.1"
                     /translation="MQKKCYRYSLCFQQSVVQAIEKDGLSISEARRRYNIAGGQTIQN
                     WVRKFGKNHLLNKVITVSTLSERDELSRLKTELKTLKIAYAELSMDHKCSEKVIELAD
                     EMFGMDLKKKYEQELSQNSVKQSK"
     gene            complement(1728..>2038)
                     /locus_tag="VF870_13120"
     CDS             complement(1728..>2038)
                     /locus_tag="VF870_13120"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253180.1"
                     /translation="TPQAMPGFDTNPLQKGNLCYGVDLRKKNQPLGIDLEWLISFYKL
                     SAQGQTFFSSPGFMDKLAGGDMLRKQISEGKTADEIRKSWQPDLEKYKTMRKKYLLYS
                     "
BASE COUNT          574 a          431 c          358 g          675 t
ORIGIN      
        1 gtttcggttg gtaagacatt tgcgacagcc ggcaattatt ggttgattgg cgaagcctcc
       61 aatggaacga caaccgttcg ggacagcgtc tggatggtgg ttaagaaaga ggtggaaacg
      121 cttaccagac cgtcaaacac ccgtgccgga atcaatgttc aggatgcctg tcatgtccta
      181 aaaaaagtgg acacttttta ctggttaata taactgtaaa attgacatac ttttgttctt
      241 tctttgtttt gatgaatttg taaacatctt tgaaaaaccg gaaaaagtct tgtcaaggtt
      301 ttgcgaaaaa atactacccg cagggtggag attttttcag caaaccgaag gcttgacctt
      361 gacacggctt ttgacgggtt ttacctttgt ttactaattg aacaaaacag caacgttctt
      421 tgatattttg tttatgctgc cttctgaata tgcacctctt gtggcgtttt tagtttcaaa
      481 gcccaatgcg gacgcagctc attatatgtc ctaattgctt cttttacaga tttgtgagtt
      541 gcttcataat ccttaaaagt ttcatccaag agaaattcat ctttcagtat gccatttacg
      601 cgttctgcct ttgaattttc ataacaatga ttttcttccg tcatactgat ttgaattgta
      661 tttttcttta aaatctttac atactcatta ctgcaatact gtatgccccg gtcggaatga
      721 tgaatcaggg gcttgtcctt atcgcgctgt cttaatgcca ttttcagggc ttcaattccg
      781 ccttcgatag ataaactatc agataacgac caacccacga ttttccgcga aaaataatcg
      841 gttatcaaaa acaaatagac gaatcctttt tctgttcgta aataagttat atccgaaacc
      901 caacaagcat tcggagtctc tatctcgttg ttatacagca agtttttata tttataaaaa
      961 cgatggtagg aatcggtagt gcgtgtaaaa ctctttttgc gttcaaccaa caattcgttg
     1021 gaacgcagaa tgtcgatgaa tttatcacga cctatttttc caatctcacc tatatcgtct
     1081 ttgagcatat aatacaattt ctttgcgcca acacgaggca tccgtttgcg gatattcaaa
     1141 accaattcga taatcacgga ttcacgaagc atctcttttt caacactctg caatgaagag
     1201 taatagccac tgcgtgaaat gccaaaatag gcacagctct ggtttacttg gactgcttta
     1261 cggagttctg agatagctct tgttcgtact ttttttttaa gtccattccg aacatttcat
     1321 cggctaattc tatcactttc tcagaacact tgtgatccat agacagctca gcataagcaa
     1381 tctttaatgt ttttaattct gttttcaatc gagacaattc gtctctttct gataatgttg
     1441 acactgtaat gactttgttt aataaatgat ttttaccaaa ttttcgaacc cagttctgta
     1501 tcgtctgacc tcctgcgata ttatatcgac ggcgcgcttc actgatactt aagccatcct
     1561 tttcaatggc ttgaactaca ctttgctgaa agcaaagact gtaccgataa cattttttct
     1621 gcatttttgt cttgtcttat gcgtaaaaaa tgtaaactta tttcaggaca agacacaaaa
     1681 aactgacaac tgataactaa cgacttacaa ctgttaactg ctaactgcta actgtacaac
     1741 aagtatttct tccgcatcgt cttgtacttt tccaaatccg gctgccatga ttttcgtatt
     1801 tcatcagccg tttttccctc actaatttgc ttgcgaagca tatcgccccc tgctaattta
     1861 tccataaaac cgggagaaga gaaaaaggtt tgtccttgag cggataattt ataaaaacta
     1921 atcagccatt ccaaatcaat gccgagcggt tgattcttct ttctcaaatc aacgccgtaa
     1981 cacagattac ctttttgcaa cggattcgta tcaaatccgg gcatagcttg aggtgtga
//
LOCUS       DASWBY010000722         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_257005, whole genome shotgun sequence.
ACCESSION   DASWBY010000722 DASWBY010000000
VERSION     DASWBY010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257005"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1509
                     /locus_tag="VF870_13125"
     CDS             <1..>1509
                     /locus_tag="VF870_13125"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253181.1"
                     /translation="KKGNEILNAPDLKFSVSVKSPKNKEVYSGEAATNEFGSLSGSFL
                     LDGEADLGYYSIIFTKDGISYYGSFTVEEYKKPEYKVNVSTDRNQYANGDKIAATVSA
                     DYYFGSPVKNASVQVNIYRQYYWRPWWYWSEWSWFYKGFRNDRYYGGQQELIHQASGV
                     LNDEGKFVLNYKVDSDKNYDYQYIISAQVTDASRRAITGSTQIFVTRGSFTISTSPDR
                     WFFQQGKTVKLKVNASDFSDKPMQTDFKVIINYPDDPKSMRMKSQSDTLFAKTNEAGS
                     AVVNFSPKQDRTGYYNYRVIAFDEKEREIEASSSFYIGDYNNYYYQRTNAGLEIITDK
                     DSYEKGDSLIAYIFLPNPGQELLLTYETDKILSYKKLKPENNSFEIREKLTDEFSPSF
                     NISVSFIKDRMLYQTTKLVGVLAKDKFLNITLNPSKETYKPGEQAEYQIIVKDYLGNP
                     VQNTEVSFGIIDESIYAIKEDETQPIETFFYSPQYSYLPTYNSLQSGYFSSY"
BASE COUNT          537 a          242 c          277 g          453 t
ORIGIN      
        1 caaaaaggga aatgaaattc tgaatgcacc ggatttaaag tttagtgttt cagttaaatc
       61 gccaaagaat aaggaagttt attccggtga agctgctaca aacgagtttg gttctctttc
      121 aggaagtttc ttgctggatg gagaagctga tttaggttac tactcaatca tttttacaaa
      181 agatggaata agttattatg gttcgtttac tgttgaagaa tacaaaaaac ccgaatacaa
      241 agtaaatgtt agcacagata gaaatcagta tgccaatgga gataagattg cggcaactgt
      301 ttcagcggat tattattttg gttcaccggt taaaaatgcg agcgtacaag ttaatattta
      361 cagacaatat tattggcgtc catggtggta ttggagcgag tggtcctggt tttataaagg
      421 atttagaaat gatcgctact atggcgggca gcaggaacta attcaccagg catcaggtgt
      481 tttgaatgat gaaggtaaat ttgtattaaa ctacaaagtg gattcagata aaaattatga
      541 ctatcaatat ataatttctg ctcaggttac agatgcatca cgcagagcaa taaccggatc
      601 aacccagatt tttgttactc gtggatcatt tacaatttca acatcaccgg atagatggtt
      661 ttttcagcaa ggtaagactg taaagctgaa agtaaacgca tcagattttt ctgataaacc
      721 aatgcaaaca gattttaaag ttattattaa ttatccggat gacccgaaaa gtatgcggat
      781 gaaatcgcaa agtgatactc tgtttgccaa gacaaacgaa gctggttctg ctgtagttaa
      841 tttttctcca aaacaagata gaacaggata ttacaattat cgtgttatcg cttttgatga
      901 aaaggaaaga gaaatagaag catcaagctc attctatatt ggcgattata acaattatta
      961 ttatcaacgc acaaatgccg gacttgaaat cataaccgat aaagattcct atgaaaaagg
     1021 agactcttta attgcctata tatttttacc aaacccgggc caggaattgc tacttactta
     1081 tgaaactgat aaaattttat cttacaaaaa actaaaacct gaaaataata gttttgaaat
     1141 ccgtgaaaag cttactgatg aattttcacc aagctttaat atttcagtca gctttataaa
     1201 agatagaatg ctttatcaaa caacaaagct tgttggagtt ctagctaaag ataaattctt
     1261 aaacatcaca ctcaatccgt ctaaagaaac ttacaaacct ggtgaacaag cagaatatca
     1321 gataattgta aaagattatc ttggtaatcc agtgcagaat acagaagtca gttttggaat
     1381 tattgatgaa agcatctacg cgattaagga agatgaaacc caaccgatag aaacattttt
     1441 ctattcgcct caatattcat atttaccgac ttacaattca ttgcaatcag gttactttag
     1501 ctcttacag
//
LOCUS       DASWBY010000723         4533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_481919, whole genome shotgun sequence.
ACCESSION   DASWBY010000723 DASWBY010000000
VERSION     DASWBY010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481919"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VF870_13130"
     CDS             <1..1114
                     /locus_tag="VF870_13130"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9253182.1"
                     /translation="IEITNDSQFVSVSNGNLVDVKTENDKKTYHYKSEYPISTYLIAL
                     YSAPYKTFYDFYKGLDGKDSMKIEYYVMPEHIDQAKIDFAEHLDMMKTLSTIFGEYPF
                     IKEKYGVAEFLWNYGAMENQTITGVGYAFVSGNDFFKDTYVHELAHHWWGNSVGPKTW
                     NDIWLNEGFATYSEALYAEAKHGKDALIAKMESKFSDNFQGTLYAPKDLFGETIYDKG
                     AWVLHMLRYEIGDSSFFKSLYNYYALYKYSNASVEDFKTVCENTSGMNLDKFFDQWVY
                     TGTDNIFCLYNIAFNSDSKKECVINLNQQPQKYSEFHFPLEIKFDFVDGSSEIRKVYI
                     DGMKKEFTFKFNLEIESIQLDPDSRLLGFFEKSSLR"
     gene            1180..1902
                     /locus_tag="VF870_13135"
     CDS             1180..1902
                     /locus_tag="VF870_13135"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX9253183.1"
                     /translation="MNKTYLFISDIHLGLQSKEVEDKKEKLLVKFLEFAKDNCDELFI
                     AGDLFDYWFEYKRVYQKGNYRTLTALKDLTESGIKVHYFIGNHDFFHKDFFEEEIGVK
                     LYHEGEAFLLNGRKFFIGHGDGLVKNDLGYNILKMVLRNSFTQWLYSLLHPDLGVGIA
                     SRTSKSSRDYTTKKDYGEEDGLFDAAKLKIDEGYEFVLFGHLHKRCFNNYKNGIYINL
                     GSWIENACYGKFIDNFEIIDWS"
     gene            1918..4143
                     /locus_tag="VF870_13140"
     CDS             1918..4143
                     /locus_tag="VF870_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX9253184.1"
                     /translation="MAKSKTKKNKNKKTKWIIWGSITFIFLALSIYTISGLPSLDELE
                     NPKPVLASRVFSADGELIGQFFIENRIETDLDSLPPYVLKALIATEDRNFYDHWGVDL
                     PRFFKAMVKNLFSLSLREGASTITQQLAKNLYALKEGRENVLDKAVRKVREWISAIQI
                     EKTFTKNEIAELYLNVSYFGRSAYGIESASKVYFNKRAKDLTLPEAALFVALLKSSVN
                     YDPVARYETALQRRNQVMYNMVDTDMLSEDEYNRLKLQPIQLASERLSHVKSEAPHFL
                     EYIRQQMTSLADKYGYDLYRDGLNIYTSLDMRMQRIANRSAKKHIAEYQILFDKNWSW
                     ERNKSLLLNIVDRAIKDDGRYKIESTQDEKAKIYNQLKNDINFVDSVKKVASKIEVGF
                     VVLDPKTGMIKALVGGENQEFGRGLNHVTGIKRQPGSAFKPFVYATAIENGYNPATTV
                     LNQQFNFNGWSPSNAGNDYSGYETLRWGLANSVNVIAGRLTISDMAPPYQVVKFAQRM
                     GIKSYMDAYPSIALGTVEVTPLEMVSAMGTFVNSGVHIDPISILKIEDRNGIVLEEFV
                     PESVQAISPQTSSIIVDMMTDVINYGTGAGVRKYFQYPAAGKTGTTQKFSDAWFVGVT
                     PDLAAGVWVGFDDHRVKFTNWYGQGGRAALPIWAMFMEGAYKELKLPVTYFTLAEGVE
                     IATFCKETMNLGDSKLATDACPETYTDLVDASKMPGYCEIHTGSAGRIIHENKQGDSQ
                     W"
     gene            4216..4299
                     /locus_tag="VF870_13145"
     tRNA            4216..4299
                     /locus_tag="VF870_13145"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4250..4252,aa:Leu,seq:cag)
     gene            4263..>4533
                     /locus_tag="VF870_13150"
     CDS             4263..>4533
                     /locus_tag="VF870_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253185.1"
                     /translation="MYFRGGSSPPSGTIYIENRAKIKLYFLINEKCLLIAYLKLMMSK
                     QNGKQFFCPSFTLQNHFMDRKEVAMFLSKRPNGIYHLYYDQADGKR"
BASE COUNT         1505 a          693 c          898 g         1437 t
ORIGIN      
        1 tattgagatt actaacgatt cgcagtttgt ttctgtatcg aatgggaatc tggttgatgt
       61 taaaactgag aatgataaaa agacttatca ctacaaatca gaatacccaa tctcaactta
      121 tcttattgct ttgtattccg ccccatataa aactttttat gatttttaca aaggactcga
      181 tggaaaagat tcgatgaaaa ttgaatatta tgtaatgcct gaacatattg atcaagcgaa
      241 aatagatttt gctgaacatc ttgatatgat gaaaacacta tcaacgattt ttggtgaata
      301 tccatttatt aaagaaaagt acggcgttgc agaattctta tggaattacg gagccatgga
      361 aaatcagact attacaggtg taggttatgc ttttgtaagc ggtaatgatt tctttaaaga
      421 tacatatgtg catgaacttg ctcatcattg gtggggcaat tctgttggac caaaaacctg
      481 gaacgacatt tggctcaatg aaggctttgc aacttattct gaagcacttt atgcagaggc
      541 aaaacatggt aaagatgcat taatcgctaa gatggaaagt aagttcagtg ataatttcca
      601 aggaacactt tatgctccta aagatctttt cggtgaaact atttatgata agggtgcatg
      661 ggtacttcat atgcttaggt atgaaatagg ggacagcagt ttctttaaat cactttataa
      721 ttattatgct ctctataaat actcgaatgc ctccgtagaa gattttaaaa ctgtttgtga
      781 aaatacttca ggcatgaatc tcgataagtt ttttgatcaa tgggtttata caggtactga
      841 taacattttt tgtttataca atattgcatt taacagtgat tccaaaaagg aatgcgtcat
      901 aaacctgaat cagcagccgc aaaaatattc agaatttcat tttccgcttg aaataaaatt
      961 tgattttgtt gatggctcct ccgaaatccg aaaagtttat attgatggca tgaagaagga
     1021 gtttacattt aaatttaacc tggaaattga atcaatccaa ttggatcctg attccagatt
     1081 acttggattt tttgaaaaat catctttgcg ttaggcaatg aatatgtttt atttcttttt
     1141 gcatatctgt aaatttcaat taaataataa ttataatctg tgaataaaac ttatcttttc
     1201 atctccgata tacatcttgg tcttcaatcc aaagaagttg aagacaaaaa agaaaaacta
     1261 ctcgtaaagt ttcttgagtt cgcaaaagat aactgcgatg aactttttat tgcaggcgat
     1321 ctttttgatt attggtttga gtacaaaaga gtttatcaaa aagggaatta tagaacttta
     1381 acagctttaa aggatctaac tgaaagcgga attaaagttc attactttat tggtaatcac
     1441 gatttttttc ataaagattt ttttgaagaa gaaattggag taaaactcta tcacgaaggt
     1501 gaagcatttt tattaaacgg cagaaagttt tttatagggc acggagatgg gcttgtaaag
     1561 aatgatcttg gttataatat tttgaaaatg gttttacgta atagttttac acaatggtta
     1621 tatagtttac ttcaccctga tttgggagtt ggaattgcaa gcagaacaag taaatcgagt
     1681 cgtgattata ccacaaaaaa agattacggt gaagaagatg gtttatttga tgctgcaaaa
     1741 ttaaagatcg atgaaggata tgaatttgtt ttattcgggc atttgcacaa aaggtgtttt
     1801 aacaattata aaaacgggat ctacattaat ttaggttcat ggattgaaaa tgcctgctac
     1861 ggaaaattta ttgacaattt tgaaattata gactggagtt aaaagaataa ttggattatg
     1921 gctaaatcaa aaactaaaaa aaataagaat aaaaaaacta aatggattat ttggggatcg
     1981 ataacattta tctttttagc tttatctatt tatacaatta gcggtttgcc ttctcttgat
     2041 gaacttgaaa acccaaaacc ggtacttgca agccgggttt tttctgctga tggtgaattg
     2101 ataggacagt tctttattga gaacaggatt gaaactgatt tagacagcct tcctccttac
     2161 gttttaaaag cattgatcgc aactgaggac agaaattttt atgatcattg gggtgttgat
     2221 cttccccgat tctttaaagc gatggttaaa aatcttttct cactatctct tcgcgaaggt
     2281 gcaagcacaa ttacccagca gcttgcaaaa aatctttacg cactaaaaga aggccgggaa
     2341 aatgtgcttg ataaagccgt tagaaaagta cgtgagtgga tatcagcaat ccagatagaa
     2401 aagaccttca ctaaaaatga aatagcggaa ttatatctta acgtttccta ctttggtaga
     2461 agcgcttatg gaattgagtc tgcctcaaaa gtatatttta ataaacgggc aaaagattta
     2521 acacttcccg aagccgcatt atttgttgcg ctgctaaaat cttccgtaaa ctatgatcct
     2581 gttgcaagat atgagacagc attacaaagg cgtaatcagg taatgtacaa tatggttgat
     2641 acagatatgc ttagcgaaga tgaatacaat cgattgaaat tgcagccgat tcagctggca
     2701 tcagaaagac taagccatgt aaaatctgaa gcgcctcatt ttctggagta tattcgccag
     2761 cagatgacat ctcttgcgga taaatacggt tacgatcttt acagggatgg attaaatatt
     2821 tatacttcgc ttgatatgag aatgcagagg attgcaaatc gatctgcgaa aaaacatatc
     2881 gccgagtatc aaatattatt tgataaaaac tggagttggg aaagaaacaa aagcttactt
     2941 ttaaatatcg tcgatagagc aattaaggat gatgggcggt ataaaattga atccactcaa
     3001 gatgaaaaag caaaaatcta taatcagctt aaaaatgata tcaattttgt tgattcggtt
     3061 aaaaaagttg catccaaaat agaagttggc tttgtggttc ttgatcctaa aacaggtatg
     3121 attaaagctc ttgttggtgg tgaaaatcag gaatttggaa gaggacttaa tcacgtaacc
     3181 ggaataaaaa gacaacccgg atcagctttt aaaccttttg tttatgctac tgcaattgaa
     3241 aatggttata atccggcaac aacagtttta aatcagcaat tcaattttaa tggatggtcg
     3301 ccatctaatg ccggtaatga ttatagtggc tacgaaactt tacgatgggg tttagcaaac
     3361 tctgttaatg ttattgcagg acgacttaca atcagtgata tggctccccc atatcaggtt
     3421 gtaaaatttg cacagaggat gggtataaaa tcttatatgg atgcttatcc atcaatagca
     3481 ttgggtaccg tagaagttac tcctcttgaa atggtttctg cgatgggaac atttgttaat
     3541 tccggtgttc atatcgatcc aatttctatt ctaaagattg aagacaggaa tggaattgtt
     3601 cttgaggaat ttgttcctga atcagtccag gcaatttctc ctcaaacatc ttctataatt
     3661 gtagatatga tgacggatgt aattaattat ggaaccggtg ccggagtaag aaaatacttc
     3721 caatatccgg ccgctggtaa aacaggaacc acacaaaaat tttctgatgc atggtttgtc
     3781 ggtgttactc cggatttagc tgcgggtgtc tgggttggat ttgatgacca tcgagttaaa
     3841 tttacaaatt ggtacgggca gggaggccgg gccgctttac ctatctgggc aatgtttatg
     3901 gaaggtgctt acaaagaatt aaaacttccg gtaacatatt ttacacttgc ggaaggtgtt
     3961 gaaattgcta ctttctgtaa ggagaccatg aatcttggag attcaaagtt agcaacagat
     4021 gcctgtcccg aaacttacac agatttagtt gatgcaagta aaatgcccgg ttattgtgaa
     4081 atacacacag gcagtgctgg acgaataatc cacgaaaaca aacaaggcga tagccagtgg
     4141 taagtttgat gcagaatgaa taatttagaa tgtagaattg aaatttttta cacattctaa
     4201 attcttaatt gttttgcccg gatggcggaa ttggtagacg cgctaggttc agggtctagt
     4261 ggatgtactt ccgtgggggt tcaagtcccc cttcgggcac aatatatatt gaaaatagag
     4321 ctaaaatcaa gctttatttt ctcataaatg agaaatgctt actaattgct tacttaaaat
     4381 tgatgatgag taagcaaaat ggtaagcaat ttttttgccc atcttttaca cttcaaaatc
     4441 attttatgga cagaaaggaa gtcgctatgt ttctttctaa acgtccaaat ggtatttatc
     4501 atctctatta tgatcaggct gacggcaagc gta
//
LOCUS       DASWBY010000724         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_257118, whole genome shotgun sequence.
ACCESSION   DASWBY010000724 DASWBY010000000
VERSION     DASWBY010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257118"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..>1766
                     /locus_tag="VF870_13155"
     CDS             87..>1766
                     /locus_tag="VF870_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX9253186.1"
                     /translation="MSTSSQKFYLIILTGILLISYPLFTRAQDKQLSFNQVYLFGEPR
                     ILKSFPRLQGWFDDEHYLMQKRDATTSAIIKVNAKTGDETVVLDFNSINPNLDEAELT
                     AEENIGITKDYTGLLFYDDNDLFFYSIPQNKVTRLTNDKDQEVNPMLSPDGKKVAYTK
                     NKDLYVVDVESSIETRLTNDASDVVYNGYASWVYMEEIIGRSLNYRAFWWAPNSEMIS
                     FLRFDDTPVPKFPLFNANGVHGELEWEHYPKPGDPNPIVKMGIAHINKDKIIWIEEDE
                     NADQYTAWPFWSPDSKEMFYQVLNRDQNDLQLLSANPETGKNRLVYEEKSNNWVEFFE
                     NINILKNNKGFILRSEVDGWRHLYYYDMNGNLKKQLTKGEWNVSDIKLVDEENQKIYF
                     EANKGERLETHLFVVDFTGDDLEQLTTIAGTHKANVSPNGKYFYDSYSNIDTPGKLDL
                     CNGDGDLIKNLGDRKSKIFDEYNLGKTELFSIKTDDGFDLPAMWILPPDFDESKKYPV
                     LFGVYGGPGGTDVKNSFSAYLDRYFISQSEIIYFVVDHRGSAYFGKKGKDFI"
BASE COUNT          612 a          272 c          320 g          562 t
ORIGIN      
        1 ttttaatttg caataatctc ttaatcaatc catttttata tgtaaagatt ataaagttat
       61 ttccaaaact ctcttctgga gaatttatgt ccacatcatc acaaaagttt tatttaataa
      121 ttcttaccgg aattcttcta atttcatatc cactttttac cagggctcag gataaacaac
      181 tatcgtttaa ccaggtttat ttattcggtg agcctagaat tttaaaatcg tttccacgtc
      241 tgcaaggctg gtttgatgat gagcattatt taatgcaaaa acgtgatgca accacaagtg
      301 caattataaa agttaatgcc aaaacaggag acgaaactgt tgtgctcgat tttaattcaa
      361 tcaatccaaa tcttgatgaa gcagaactaa cagcagaaga aaatatcgga ataacaaaag
      421 attataccgg cttgcttttt tatgatgata acgatttgtt tttctattct attcctcaaa
      481 acaaagtaac ccgattaaca aatgataaag atcaagaagt taatccaatg ctttcaccgg
      541 atggaaaaaa agttgcttac acaaaaaata aagatcttta tgtggttgat gttgaatctt
      601 caatagaaac gcgtttaacc aacgatgcca gcgatgttgt ttacaatggt tatgcttcgt
      661 gggtttatat ggaagaaatt atcggtcgct cacttaatta tcgcgctttc tggtgggctc
      721 caaacagtga gatgatttct tttttaagat ttgatgatac accggttcct aaatttcctc
      781 tgtttaatgc caatggtgtg catggtgaac ttgaatggga gcattatcca aaaccaggcg
      841 atccaaatcc aatagtaaaa atgggtatcg cacacatcaa taaagacaaa attatctgga
      901 ttgaagaaga tgagaatgca gatcaataca ctgcgtggcc attctggtcg ccggacagta
      961 aagaaatgtt ttatcaggtt ttaaatcgtg accaaaatga tttgcaactt ttatctgcaa
     1021 atcccgaaac cggaaaaaac agattagtat atgaagaaaa aagtaataac tgggttgaat
     1081 tctttgaaaa cattaacatt ttaaaaaaca ataaaggatt tatacttcgc tccgaagttg
     1141 atggctggag acatctttat tattacgata tgaatggaaa tttgaaaaaa caattaacaa
     1201 agggcgaatg gaatgtttct gacatcaaac ttgtagatga agaaaaccaa aaaatatatt
     1261 ttgaggcaaa caaaggtgaa agattagaaa cacatttatt tgttgttgat tttactggag
     1321 atgatttaga gcagcttaca actattgcag gcacacacaa ggctaatgtt tctcctaatg
     1381 gaaaatattt ttacgattct tattcaaaca tcgatacacc tggaaagtta gatttatgta
     1441 atggtgatgg tgatctgata aaaaatctag gcgatagaaa atctaaaata tttgacgaat
     1501 acaacctcgg caaaaccgaa ttgttttcaa ttaaaactga tgatggtttt gatttaccgg
     1561 caatgtggat tttgcctccg gattttgatg aaagtaaaaa gtatccggtc ttgtttggtg
     1621 tttatggtgg tccgggggga acagatgtta aaaattcttt ctccgcttat ttagatagat
     1681 attttatttc acaaagcgaa ataatttatt ttgtagttga tcatcgcggc tcagcttact
     1741 ttggaaaaaa aggtaaagat tttatc
//
LOCUS       DASWBY010000725         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_517373, whole genome shotgun sequence.
ACCESSION   DASWBY010000725 DASWBY010000000
VERSION     DASWBY010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517373"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VF870_13160"
     CDS             complement(<1..1056)
                     /locus_tag="VF870_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9253187.1"
                     /translation="MLNLLIIGFIASAIAPLIYKPLKNYFGWIAASFPLYMFISFFLN
                     YDEISSGKIIKEATEWIPSLGVNFSFLLDGLSLTFVLIITLIGTAVFLYAGSYMKDYE
                     YTGRFYLYIGVFMTSMIGLVLSDNMITLFVFWELTSISSYLLIGFNHHKESSRKSALQ
                     ALLVTGGGGLALMAGLILLAQISGSYEVSALYNMHSTIVGSSLYPAIVILVLLGAFTK
                     SAQFPFHFWLPNAMEAPTPVSAYLHSATMVKAGIYLIARLNHAIGGTVLWQDTLLVAG
                     TITMFIAALLAFKQIDLKKLLAYSTLSVLGTLTMLLGIGSNLAIKAFFIFLVAHSLYK
                     GTLFLVAGTIDHETGTRD"
     gene            complement(1205..1519)
                     /locus_tag="VF870_13165"
     CDS             complement(1205..1519)
                     /locus_tag="VF870_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEX9253188.1"
                     /translation="MKVIVDLCVVPIGVGLSVSKYIAECEKILKQAGLNTMLHAYGTN
                     IEGEWDDVFAAVKKCHERVHEMGAPRISSTLRVGTRTDREQTMDDKIKSVQLKIDKDK
                     IA"
     gene            complement(1601..>1942)
                     /locus_tag="VF870_13170"
     CDS             complement(1601..>1942)
                     /locus_tag="VF870_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX9253189.1"
                     /translation="ELALPLALVGIGGSLNTENLKRASNLAISSSIIKIVIIPLVLTV
                     GALLLGYRNDDLGIVFIVFAAPTAIASFVMADVMGANSRLAGNIIMITTLGSVFTISI
                     GILLLKSFNLL"
BASE COUNT          686 a          351 c          331 g          574 t
ORIGIN      
        1 atctcttgtt cctgtttcgt ggtcaatagt tccggcaaca agaaataaag ttcctttata
       61 taatgaatgc gcaaccagaa aaatgaagaa agcttttata gcgagatttg aaccgattcc
      121 caaaagcata gttaatgttc caagaacact taaagttgag taagctaaaa gctttttcag
      181 atcaatttgt ttgaatgcaa gtaaagcagc aatgaacata gtgattgttc ctgcaacaag
      241 aagtgtatcc tgccacaaaa cagttccgcc aattgcgtga tttaatctgg caattaaata
      301 aatcccggct ttaaccattg tagcagaatg taaataggca ctaaccggag ttggtgcttc
      361 cattgcattt ggaagccaga aatgaaaagg gaattgtgcg gattttgtaa atgcaccaag
      421 caaaactaaa ataacaattg caggataaag acttgaacca acaattgtag aatgcatatt
      481 gtacaaagca gaaacctcat agctacctga gatttgggca agaagtatca agccggccat
      541 taaagcaagt ccgccgcctc ctgttacaag taaggcctgt aaagctgatt ttcttgatga
      601 ttctttatga tgattgaaac caattaataa ataggaactg atgctcgtca attcccagaa
      661 aacgaaaaga gtgatcatat tatcagaaag aacaagtccg atcatcgaag tcataaacac
      721 accgatgtaa agataaaatc ttccggtgta ttcgtaatct ttcatataac ttccggcgta
      781 aagaaaaaca gcagttccga taagtgtaat aattaaaaca aaggtcagac ttaatccatc
      841 aagcagaaac gaaaagttaa caccaagaga gggaatccat tcagtagctt ctttaataat
      901 tttcccggat gagatttcat catagttcaa aaagaagctt atgaacatgt aaagcggaaa
      961 ggatgcagca atccagccaa aataattctt cagcggcttg tatattaaag gtgctattgc
     1021 tgacgcaata aatccaatta ttaaaagatt gagcatctaa actttttact tagtaaaatt
     1081 ttgataatga ttttaataca tttagaaaag ctaaaaacaa aacttaaagt cctctgtttt
     1141 agttccttaa gataatgaat taacggaaaa atgtgagata aatttccttt aataaattaa
     1201 aatatcaagc aattttatct ttatcgattt ttaattgtac gcttttgatt ttgtcatcca
     1261 tagtttgttc ccgatcagtt cttgttccaa ccctaagtgt tgaggaaatt cttggcgcac
     1321 ccatttcatg aacccgttcg tggcactttt taaccgcggc aaaaacatca tcccattcac
     1381 cttctatatt agttccataa gcgtgaagca ttgtattaag acctgcttgc ttcaaaattt
     1441 tttcgcactc ggctatatat tttgagacag agagtccaac gccgattggc acaacgcata
     1501 gatcaactat tactttcata gttacttcct tcgattttaa aatccaatat aatgtttaaa
     1561 tgttattttg taaaaagatt cgtaatgtgg taatgggtag ttagagcaga ttaaaagact
     1621 ttaacaataa aataccaatc gaaattgtaa atactgatcc aagagttgtt atcattatta
     1681 tgttacctgc aagtctggaa tttgcaccca ttacatctgc cattacaaaa cttgcaatcg
     1741 cagttggagc agcaaacaca ataaaaacaa tacctaaatc atcattacga taacctaaga
     1801 gcaaggcgcc aaccgttagc actaatggta ttataactat ttttataata gatgaactta
     1861 ttgccaggtt tgaagcacgt tttaaattct ctgtattaag tgagccgcca attcctacca
     1921 atgcaagagg aagtgcaagt tc
//
LOCUS       DASWBY010000726         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_625536, whole genome shotgun sequence.
ACCESSION   DASWBY010000726 DASWBY010000000
VERSION     DASWBY010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625536"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VF870_13175"
     CDS             complement(<1..488)
                     /locus_tag="VF870_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX9253190.1"
                     /translation="MKDKSSILFYILGSFFVANAILAEFIGVKIFSLESTFGFEPLNL
                     TLLGIENLSFNLTAGVLLWPIVFVMTDIINEYYGRKGVRFLSFTAAILIAYAFLMVFL
                     AMNLSPASFWIDRITPFGKVNMHLAFNTIFGQGLWIIIGSLTAFLVGQFVDVTVFHYF
                     KS"
     gene            complement(595..1989)
                     /locus_tag="VF870_13180"
     CDS             complement(595..1989)
                     /locus_tag="VF870_13180"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX9253191.1"
                     /translation="MKKLIIAAVLIVFSLSASADANANYAKRVGGYFYTSLSPYGTWI
                     EIGFGTPVWRPTLMKRNWSPYSQGQWIYTNYGWYWNSYEPFGDVVYHYGRWYYDDYYG
                     WIWIPDYEWAPAWVEWRYDDDYIGWAPLSPYAVFSINIGIHYTYDYYVPYYHWNYVQY
                     NHFCDNNVYNYYVAPKYKYRVHNKTKMRTNYSYYDGRVRNDGVGYNEIRERSGRTIEK
                     RDLVGVSDPRELTKDRDFKRRDTEIRTYFVNKDDISRDGLRDVKVEKKDRKSTLELSK
                     VELGRDRNNDRIDNRKLENREKDLTLDSHERSKDTREQTNNDIIRRSVEDEKKIQEQK
                     DLDKRNSDIEKRNNEQIKRNDEIRKSEEVKRIEQNKRNEQVDNHRSTNVEMKKRIEET
                     IQKRQEKKSNTEVFKQNSERKVEQKKETQRVEQRNNNSVNRNTEVKRNDTNNKTETKG
                     NTRTSEKDNKTKSR"
     gene            complement(2131..>2455)
                     /locus_tag="VF870_13185"
     CDS             complement(2131..>2455)
                     /locus_tag="VF870_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253192.1"
                     /translation="ASKGLFYGNHLLLLKLVLIFLFYLSLIIQPILGLFSSPKFLLTF
                     LISFLIKLSTEYLVLKKGTNLLFDSKVLKPFLITEILQVPYILVSGFMGMFGNLVWKN
                     RKISR"
BASE COUNT          751 a          447 c          343 g          914 t
ORIGIN      
        1 aaagacttga aataatggaa aaccgtaaca tccacgaatt gcccgaccaa aaatgctgta
       61 agcgatccaa taattatcca taatccctgc ccaaatatcg tattgaatgc taaatgcata
      121 ttaacttttc caaatggcgt aattctatca atccaaaacg aagcgggcga taaattcatt
      181 gctaagaata ccattagaaa agcataagct attagaattg cagctgtaaa tgataaaaat
      241 cttactcctt ttcttccata gtattcgttt ataatatcgg tcatcacaaa tacaattggc
      301 cacaacaaaa ctccggctgt aagattaaat gaaagattct caattcctag aagtgtcaga
      361 tttaaaggtt caaatccaaa tgtggattcc agtgaaaaaa tttttacacc aataaattct
      421 gctaatatgg cgtttgcaac aaaaaatgat ccaaggatat aaaacagaat tgaagatttg
      481 tctttcatac tttactcaaa tttttacgga aaattattct aattacttgt taaaacaaaa
      541 aagcggttcc ggaaattccg aaaccgcttt atattggaga gagttgattt aagtttatct
      601 gctctttgtt ttgttatctt tttctgaagt cctggtgttt cctttcgtct cagttttatt
      661 atttgtgtca tttcttttta cttcagtatt tctgttaaca ctattattat ttctttgctc
      721 tactctttgt gtctcttttt tctgttcaac ttttctctca ctattctgtt tgaagacttc
      781 agtattagat tttttctctt gtcttttctg tatcgtctct tcaattcttt tcttcatttc
      841 aacattagtg cttctatgat tatcaacttg ttcatttctt ttattttgtt cgattcgttt
      901 tacttcttca ctttttcgta tttcatcatt tctttttatt tgttcattgt ttcttttttc
      961 gatatcagag tttcgtttat ctaaatcttt ttgttcctga atctttttct cgtcttcaac
     1021 acttcttctg attatatcat tattagtctg ttctctagta tccttagatc tttcatgaga
     1081 atctaaagtg agatcttttt ctctgttttc aagttttcga ttatcaattc tatcgttatt
     1141 tctatctctt cctaactcaa ctttagatag ttcaagagta gattttctat cttttttctc
     1201 aactttaaca tctcttaaac catcgcgaga gatatcatct ttatttacaa agtaagttct
     1261 tatttctgta tcacggcgct taaaatctct atctttcgtt agttctcttg gatcgctaac
     1321 accaaccaga tctctttttt caatagttct cccgcttctt tcgcggattt cattatagcc
     1381 aactccatca tttctaactc ttccatcata atatgaatag ttagttctca tctttgtttt
     1441 attatgaact cgatatttgt atttaggagc aacgtaataa ttgtaaacat tattatcaca
     1501 aaagtgattg tactgaacat aattccaatg atagtaaggg acgtaataat catatgtgta
     1561 gtgtatccca atatttatgg aaaacactgc ataaggcgat aaaggtgccc agccgatata
     1621 atcatcatca tatctccatt caacccatgc aggtgcccat tcataatcag gaatccaaat
     1681 ccatccgtaa taatcatcgt agtaccatct tccatagtga taaacaacat ctccaaaagg
     1741 ttcatatgaa ttccagtacc aaccgtaatt cgtgtaaatc cactgtcctt gagaataagg
     1801 tgaccagttt cttttcatta atgttgggcg ccaaacaggc gtaccgaaac ctatttctat
     1861 ccaggttccg tatggtgaga gtgaggtgta aaaatatcca ccgactcttt ttgcatagtt
     1921 tgcgtttgca tcagcgcttg ctgataaact gaaaactatt aagactgctg cgattattaa
     1981 ctttttcatg actttctcct ttgtgtcttg ctgctattag ccaaggttta tgccagtaaa
     2041 aagttctgtt ttttaattag aaatgcacta ttagatgtta aattgtatag ttaagtgaac
     2101 agtgatgtat ttgagagatt tcaaaaaaga ttagcgactg atttttctat ttttccaaac
     2161 gagattacca aacattccca taaaaccgga aactaaaata tatggaactt gtaaaatttc
     2221 tgttatcaaa aaaggcttca aaacctttga atcaaatagt agatttgtcc cttttttaag
     2281 aactagatat tctgtggata atttaatcag aaatgaaatc aaaaatgtta ataagaattt
     2341 tggagatgag aacaatccaa gaattggctg gataattaat gaaagataaa ataaaaatat
     2401 aagaacaagt ttcagaagca aaagatgatt accataaaac aaccctttgc ttgcc
//
LOCUS       DASWBY010000727         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_744254, whole genome shotgun sequence.
ACCESSION   DASWBY010000727 DASWBY010000000
VERSION     DASWBY010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744254"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VF870_13190"
     CDS             complement(<1..1488)
                     /locus_tag="VF870_13190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9253193.1"
                     /translation="MLEIQKKLTKTFYAMLALPATAVGFALSTQIAALSWILSKKYGL
                     NIHEVAFVWLAGPLAGIFGQVIVGMISDNVWFLGGRRRPFIMVGGIVGALMFLALPQI
                     GVISKATGITSIILIASVIALLLDLSVNVTFNPARSIIADLTPEGKKRTSGYVWMQVI
                     SGTFGVLAYFLSMVYGNETLLLIAAVFVFSCSVFPILLIEEPKELAGHEEEKKDSHSV
                     LDIFKSIFPLYGFLVFGIFSLIFHFYQTELRFIHNPLLILSLAYTMIIGGWIIYKSKN
                     SESDTAEFQKIMLAHTFTWIAFQSMFVMSGFFIDKQIIPNLDLTNVFANKFAEFLTGV
                     MQTKDTTTGNIVSLGFLILNAVGAVFPAVLSIAAKRIGRVRTYTTALAFSAIGYFYIA
                     FFGRYEINFYLGMFLTGIGWSAVISIVFSIVTERINQAKMGLFMGIFNLAVVLPQMMS
                     QGVANIISETQDFQLLYILCGVFICFSVFFWFFVKEPRSTALESAV"
     gene            complement(1511..>3115)
                     /locus_tag="VF870_13195"
     CDS             complement(1511..>3115)
                     /locus_tag="VF870_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9253194.1"
                     /translation="DLFIPPTIKTKYNFEIGFARGDQDTVINYPIFAKEESNNKFKGF
                     NQSDVMYLIMADRFCDGNPDNNNIDDSLDQFTSKDLDGRKGGDIEGITSKLDYLKDLG
                     ITSIWITPMLENNMWMSYHGYAATNLYKIDPRFGSNELYKQLVAKAHDKGLKIIMDHV
                     SNHVGINHWWMKDLPLADWINGTVENHLPANHNKMTFPDPYSPGESVDLTWNGWFENY
                     MVDLNQANPFLLKYLIQNTIWWIEYLGIDGIREDTYPYCNQYAMAKWNKAILDEYPEF
                     NIVGEIWTGEPPFLAAYQQKNKFGVHLDSNLPCVTDFALSDALRNYLSGKKGLEGIFN
                     TLAQDFLYYDPTNLLTFFDNHDIARGLFIANNDIDKYKIALTILLTTRGIPKILYGSE
                     IGIVGDNNHGRIRSPFPGGFENDSLNAFSSCDRTEYQNDIFSFTQKLLELRKSYNSLA
                     VGKLTHFYPFGNVYVYFRKTDDESTMIVVNSNDKDFEVDLNKYKETLAETLKIENIKT
                     NEETDITQNKKITIKRKSAEIFLLIK"
BASE COUNT         1039 a          599 c          464 g         1013 t
ORIGIN      
        1 gacagcactt tccaaagcag tagatcttgg ctctttaaca aagaaccaga agaaaactga
       61 gaaacaaatg aatactccgc aaagaatgta aagcaactga aagtcttgag tttcggagat
      121 aatatttgca acaccctgac tcatcatttg tggcaataca actgctagat taaatattcc
      181 cataaacaaa cccatctttg cctggttaat tctttcagtt acaattgaga agacgattga
      241 gatcactgcc gaccatccga taccagtcaa gaacattcca agataaaagt ttatttcata
      301 tctgccaaaa aacgctatgt aaaaatatcc aattgcacta aaagctaatg cagttgtgta
      361 agttctaact cttcctattc tctttgctgc tatcgaaagt actgccggaa aaacagcccc
      421 aacagcattt agaattagaa atcctaatga aacaatattt cctgttgttg tatctttggt
      481 ttgcattacg ccggttagaa attcagcaaa cttatttgca aacacatttg ttagatcgag
      541 atttggaata atttgtttat cgataaaaaa tcctgacata acaaacatac tctggaatgc
      601 aatccatgta aatgtatgag cgagcatgat tttctgaaat tccgctgtat cagattcaga
      661 atttttactc ttataaatga tccagccacc tataatcatt gtgtaggcaa gacttaatat
      721 caataaagga ttgtgtatga atcttaactc agtttgatag aagtggaaaa tcagactaaa
      781 aattccaaaa accaaaaagc catataaggg aaaaattgat ttaaatatat ccaggactga
      841 gtgagaatct tttttttctt cttcatgtcc cgctaattct ttcggctctt ctatcagcaa
      901 gattggaaat accgaacatg aaaaaacaaa aactgccgct atcaataaaa gtgtttcatt
      961 tccataaacc attgataaaa aatatgctaa cacaccaaac gttccagaaa taacctgcat
     1021 ccaaacataa cctgatgttc gcttcttccc ttccggtgtt aaatctgcaa ttatcgaacg
     1081 ggcaggattg aatgttacgt tcacagataa gtctaagagc aatgcaataa ctgatgcaat
     1141 taagattata ctcgtaattc cagtggcttt agaaattact ccgatttgcg gcaatgctaa
     1201 aaacattaat gcaccaacaa tacccccgac cataataaat ggtcttcttc gtcctcctaa
     1261 aaaccaaaca ttgtcgctaa tcattccaac aattacctgg ccaaatattc cagcaagcgg
     1321 accggcaagc caaacaaatg caacttcgtg aatatttaaa ccatactttt tacttaatat
     1381 ccagcttaaa gccgcaatct gggtcgataa tgcaaaacca actgcagtag cgggaagggc
     1441 cagcattgca taaaaagttt ttgtaagttt tttctgaatt tctaacatag attttacttt
     1501 cagtttgtgt ctattttatc aataaaaata tttctgctga ttttctttta attgtaattt
     1561 ttttgttttg agttatatca gtctcttcat tcgttttaat attttcaatc tttaaagttt
     1621 ctgccagagt ttctttgtat ttattcagat caacttcaaa atctttatca ttactgttta
     1681 caactatcat tgttgattca tcatcagttt ttctgaagta aacataaaca ttaccaaatg
     1741 gataaaaatg agttagcttt ccaacagcaa gcgaattgta actctttctt agttctaaaa
     1801 gcttttgagt aaaactaaaa atatcattct gatattcagt tctatcacaa ctagaaaaag
     1861 catttagaga atcattttca aatccgcccg gaaaaggaga acgaattctt ccgtgattat
     1921 tatctcccac tattccgatt tcagatccat ataaaatttt tggaatgcct cgggttgtta
     1981 aaagaatggt aagtgcaatt ttatatttat caatatcgtt gtttgcaata aacaatccac
     2041 gcgcaatatc atggttatca aaaaatgtta aaagattggt tggatcatag taaagaaaat
     2101 cctgagcaag cgtgttgaag attccttcca atcctttttt accgcttaag taatttcgga
     2161 gtgcatcaga taatgcaaaa tcagttacgc atggaagatt agaatctaaa tgtacaccaa
     2221 atttattttt ctgctggtat gctgcaagaa atggtggttc acctgtccat atttctccaa
     2281 caatattaaa ctcagggtat tcatctagaa tagctttatt ccacttggcc attgcgtact
     2341 gattacaata tggatacgtg tcctcacgaa ttccatcaat acctaaatac tcaatccacc
     2401 aaattgtgtt ttgaattaaa tactttagaa gaaacggatt agcctggtta agatcaacca
     2461 tgtaattttc aaaccagcca ttccaggtta aatcaactga ttcgcccggg ctgtacggat
     2521 ccgggaaagt cattttatta tgatttgcag gtaaatgatt ttcaactgtt ccgttaatcc
     2581 agtctgctaa cggcaagtcc ttcatccacc aatgatttat tccgacatga tttgaaacgt
     2641 gatccataat tattttcaaa cctttatcgt gggcttttgc tacaagctgc ttataaagtt
     2701 cattgcttcc aaaacgcgga tcaatcttgt ataaatttgt agcagcgtaa ccgtgataac
     2761 tcatccacat gttattttct aacatcggag ttatccagat cgaggtaata ccaagatctt
     2821 ttaaataatc taacttagaa gttattcctt ctatatctcc gccttttctc ccatctaaat
     2881 cttttgatgt aaactgatct aaactatcgt caatattatt attatcagga tttccatcgc
     2941 aaaatctatc tgccataatc aagtacatta catcagattg attgaaacct ttgaatttat
     3001 tattagattc ttctttggca aaaattggat aattaatcac agtatcttga tcccccctgg
     3061 caaatccaat ttcaaaatta tatttagttt ttattgtagg tggaataaat aaatc
//
LOCUS       DASWBY010000728         5213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_443817, whole genome shotgun sequence.
ACCESSION   DASWBY010000728 DASWBY010000000
VERSION     DASWBY010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443817"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1601
                     /gene="radA"
                     /locus_tag="VF870_13200"
     CDS             255..1601
                     /gene="radA"
                     /locus_tag="VF870_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX9253195.1"
                     /translation="MSKTKIKYICSNCGYESLRWIGKCPECESWNTFTEEIVETSPRK
                     ANISKSRFSYSKILDISANEDDRIKTGIIEFDRVLGGGIMPGSVILLGGDPGIGKSTI
                     AMQAAASIKEKVLYVTGEESEKQIKLRSSRLKVKSDSFFVQAETELSNILGAIKEISP
                     SVVIIDSIQTTYRSELENSPGTITQIRECAAMLMEEAKKNHYSVIIIGHVTKEGMIAG
                     PKILEHMVDTVLQFEGESNHSFRILRAQKNRFGSTNEIGVFEMREDGLREVTNPSELF
                     LSEREKQTPGSVVTSSIEGSRPILLEVQALVTPSNFGYPQRVSNGFDQKRLSILLAVL
                     EKRAGVRASVHNVFVNVAGGIRVIEPAVDLAVCVSIASSLIDKVVDNQTIIIGEVGLG
                     GEIRSVGHIEKRIQEAQKLGFKSAIIPSGNLKGIKQSDKIKVIAVDNVKDAITKLL"
     gene            1827..2078
                     /locus_tag="VF870_13205"
     CDS             1827..2078
                     /locus_tag="VF870_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9253196.1"
                     /translation="MSTKLFVGSLPWSASDEDLQTAFAAHGNVLSAKVVTDKETRRSR
                     GFGFVEFETEAEATAAIQALNGTELKGRNIIVSEAKPKK"
     gene            complement(2142..4550)
                     /locus_tag="VF870_13210"
     CDS             complement(2142..4550)
                     /locus_tag="VF870_13210"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HEX9253197.1"
                     /translation="MIIKTFLHLLLFTLIINAQQVTIKGKIVDAVLKSTLSFANIRVA
                     ETTLGTSSNANGEFELKIEKGTYILVASYIGYYSDTLIVNADKIINEVNFSLQPSELI
                     LPEVLVKPGVNPALEIIRKAIEKKNLRNSKLKNYEVEAYTKGLIRTTEDISAGNNSIG
                     VGLGGSDTTELIISGILENHSRNYFEQPDKFKSIILARKQSANFPPSINTLTGGRLIQ
                     NFYDNDVNFLGRDLPGPLADNALNYYYYYIENVLAQNNKTIFQIHIEPDNSADPGFSG
                     KIFITDSTFDLVKVDLILNRAANTGNLFDTVNIFQQFDEYNNIVMPVDYRLFVKANLL
                     GLVRFGFELNTIMFNYSINSNISENIFDKAIVTVLPTADNIDSLYWQGTQTIPNTVEE
                     QLAYNRIDSIESIPKKFLDDYSPLDSRISLSGIYSISAPIAMYHFSRVEGHALDFGIF
                     ADDALNKRLNSSLKLSYGFSDKKFKQDFNAGYLLGDYRTWEVKARAFNKLNVLFEESD
                     NYGELFSTLVTLFSKNEFRDYYYSKGIEIGVEGEVLPALNLRVGFRNNTDNSAYVNTN
                     FAFTNKDKTYRENPLIYETRISSFKLGFDIDFRDYIEDGYFQRRTSLGRSYFLLSGDI
                     TYSNPDFISSNIKFTTYELSAIAFIRTFKSAFMNINVYGRSTDGATPYQDLFTLPGNI
                     DVIFNPNTFRTLNVNEITGDRVFMLNFTHDFRDELFRAMNIPGIKNWEILFSLIFNTA
                     VADITNESVAILTNPVKTFKHPFYEIGFGLGQGLLPFKLEFMWKLNYRDGNNFRIGLN
                     MPLL"
     gene            4725..>5213
                     /locus_tag="VF870_13215"
     CDS             4725..>5213
                     /locus_tag="VF870_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253198.1"
                     /translation="MKNLKLFLLFIFNFAIFSQIVLFAQESGNKETIDPKIVLAKYLD
                     AIGGRDALAKVEDRTTIMRGTAMGQNLTIIAKQKAPNKMRQEVKAGGMDQTIIFDGEK
                     GVMKAATQTIDITGKELEQLKIEASMELMLDPESFGIKLSYEGTEKIHDKDAYKIKMT
                     LPS"
BASE COUNT         1740 a          863 c          952 g         1658 t
ORIGIN      
        1 gttgaataaa ttgttggaag ttttttgtag tttaatttaa ctatgttttt tgttacgcaa
       61 ttcttatata gtcattgcaa atgagtcttc gaacgaagca atctcaaaat aaagcagctc
      121 aaaaagttgt tcagattgct tcacgcgcta aatgaggatt cgaaataagg ggataaattt
      181 ttaataacgt ttccaaagat taaagctgtt ttaataattt tttttgacac ttcacttttg
      241 accattgaca tattatgagt aaaacaaaaa taaaatacat ctgttctaac tgcggttatg
      301 aatctttgcg ctggataggc aaatgcccgg agtgcgagag ctggaacacc tttacagaag
      361 aaatcgttga aacttctcct cgcaaagcaa atatctcaaa atcaagattt agctacagca
      421 agattttgga tatctctgct aatgaagatg acagaataaa aacaggcata atagaatttg
      481 atcgtgtgct tggcggtgga ataatgcccg gctcggttat tctgcttggc ggtgatcccg
      541 gaattggtaa atcaacaatt gcaatgcagg ccgccgcaag cattaaagaa aaagttttat
      601 atgtaacggg tgaggaatct gaaaaacaaa ttaaacttcg ttccagcaga ttaaaagtaa
      661 aatcagattc attttttgtt caggctgaaa cagagttatc aaatatactt ggagcaatta
      721 aggaaatctc gccttctgtt gtgattatag attctatcca aacaacctac agaagtgaat
      781 tagaaaactc acccggaacg attacacaaa tacgtgaatg tgcggcaatg ctgatggaag
      841 aagcaaagaa aaatcattac tctgtaataa taataggaca tgttacaaaa gaaggaatga
      901 ttgcaggacc aaaaattctt gagcacatgg ttgataccgt tttacagttt gaaggagaat
      961 ccaatcactc attcagaatt ttacgcgcac aaaaaaatag atttggcagc acgaacgaaa
     1021 ttggtgtttt tgaaatgcgc gaggatggat tacgtgaagt tacaaatccc agcgaattat
     1081 ttttaagcga aagagaaaaa caaactcccg gctctgttgt aacatcaagt attgaaggaa
     1141 gcagaccgat tcttttagag gtgcaggcat tggtaactcc atcaaacttt ggatatccgc
     1201 agcgagtttc aaatggattt gatcagaaga gattatcaat tcttttagct gtccttgaaa
     1261 aaagagccgg cgttcgcgct tctgtgcata atgtttttgt aaatgtcgcg ggtggtatcc
     1321 gtgtaataga accagctgtt gatcttgctg tctgcgtaag catcgcatca agcttgattg
     1381 ataaagtagt tgataatcaa actataatta ttggtgaagt tgggttaggc ggagaaatac
     1441 gaagcgtcgg gcatattgaa aaaagaattc aggaagcgca aaaactaggg tttaaatctg
     1501 ctattattcc ttctggaaat ttaaagggaa tcaagcaatc cgataaaata aaagtgattg
     1561 cagttgataa cgttaaagat gctatcacaa aattgctttg atttttaagt attggcctga
     1621 cgattgtctg aattcaaaaa ccttaaatta tttcaaagat tcaccccaat atcctattaa
     1681 aaagcatcaa gatgagacag aaagccctgt ttaaggctta aaatgaagga ttaattttat
     1741 tttttctttg tttctttaaa taataatatt attttattcc cgcgtcgatc gacgtttttt
     1801 tgttcaatta attaaaggtt cgtttcgtga gtactaaatt gtttgtaggg agtctcccct
     1861 ggtcagctag tgacgaagat ttgcagactg catttgccgc acatggcaac gtactttcag
     1921 caaaagttgt tactgataaa gagacccgca gatcacgagg ttttggtttc gttgagttcg
     1981 aaactgaagc tgaagccact gcggctattc aggcactaaa tggtactgaa ctcaagggtc
     2041 gtaatatcat cgtaagtgaa gcaaagccca agaaataagt tctgtttttt tccttttgag
     2101 cgctcttttt agagcgcttt tttttgccca aatttgcata cttacaacaa aggcatattc
     2161 aaaccaattc tgaaattatt tccatcacgg taattcagct tccacataaa ttcaagttta
     2221 aatggaagta agccctgtcc taaaccaaaa ccaatttcat aaaacgggtg cttgaaagtt
     2281 ttaacgggat tggtaagtat agcaacggat tcatttgtta tatcagcaac ggcggtgtta
     2341 aagataagtg aaaataaaat ttcccaattt tttattccgg gtatgttcat tgccctgaaa
     2401 agttcatctc tgaaatcgtg cgtaaagttt aacataaaaa ctctatcacc ggtaatttcg
     2461 tttacgttta aagtcctgaa tgtattagga ttaaagatca catcaatatt tcccggcaaa
     2521 gtgaaaagat cctgataggg ggttgctcca tctgttgatc tgccgtagac attaatattc
     2581 ataaaagcag atttaaatgt tcttataaaa gctattgctg ataattcgta agtggtaaac
     2641 ttaatattac tgctgataaa atcaggattg gaatatgtta tatcacccga aagcagaaaa
     2701 taagatcttc ccaatgaagt tcttctttga aaataaccat cctcaatgta atccctgaaa
     2761 tcaatatcaa aaccaagctt gaatgaactt attcttgttt catagataag tggattttct
     2821 ctgtaggttt tatccttatt agtaaaagca aaattagtat taacataagc agagttatcg
     2881 gtgttatttc taaaaccaac acgtaaattc aaagctggta aaacttctcc ttcaactcct
     2941 atctcaattc cttttgagta ataataatcc ctgaattcat tttttgaaaa caaggtaaca
     3001 agcgtagaga ataattcgcc gtaattatca gactcttcaa ataaaacatt aagtttatta
     3061 aacgctctgg ctttaacttc ccacgttctg taatctccaa gcaaatatcc tgcgttaaaa
     3121 tcctgcttaa attttttgtc cgagaaaccg taagaaagtt taagtgaaga atttagcctt
     3181 ttgtttaaag catcatctgc aaaaatgccg aaatcaagtg cgtgcccttc cactcttgaa
     3241 aaatgataca tcgcgatcgg ggcgcttatt gaatatatcc cggataaaga aatcctggaa
     3301 tcaagcggag agtaatcatc aaggaatttt ttaggaatgc tttcaatact atcgatacga
     3361 ttataagcta gttgttcctc tactgtgttt ggaatggttt gcgttccctg ccaataaaga
     3421 gaatcaatat tatctgcagt cggaagtaca gtaacaattg ctttatcaaa aatattttct
     3481 gaaatattgc tgttgataga gtaattaaac attatcgtat taagctcaaa gccaaatctt
     3541 actaatccca aaaggtttgc ttttacaaac aaacgataat caaccggcat tacaatatta
     3601 ttatattcat caaactgctg gaatatgttt actgtatcaa ataaatttcc tgtgttagcc
     3661 gcacgattaa gaatcaggtc aactttaacc agatcgaatg tgctatcagt tataaatatt
     3721 tttccggaaa agcctggatc cgctgaatta tcaggttcaa tgtgaatttg aaatatggtt
     3781 ttgttgtttt gagctaaaac attttcaatg tagtagtagt aataatttag tgcattatca
     3841 gcgagtggcc cgggcaaatc tcggcctaaa aagtttacat cattatcata gaagttttgg
     3901 attagtcgcc cgcccgttaa tgtatttatt gatgggggaa agttagcgct ctgctttctt
     3961 gcaagaataa ttgatttaaa tttatccggc tgttcaaaat aatttcggct gtgattttca
     4021 agaatgccgg aaattattaa ttccgttgta tcgcttcctc ctagcccaac tccaatgctg
     4081 ttgtttccgg cagagatatc ttctgttgtt ctgattagtc cctttgtata agcttcaact
     4141 tcataatttt taagtttaga atttctaaga ttcttttttt caattgcttt tctgataatt
     4201 tccaaagccg gatttacgcc gggttttaca agcacttccg gaagtatcaa ttcagatggt
     4261 tgcaatgaaa aatttacttc attaataatt ttatcagcat taacaattaa cgtatcagaa
     4321 taataaccaa tgtatgatgc aacaagtata tatgtgcctt tctcaatttt cagttcaaac
     4381 tcaccattag catttgatga tgttccaagt gttgtttctg caactctaat attggcaaaa
     4441 ctgagagtag acttgaggac agcatccaca atttttccct taattgtaac ttgctgggca
     4501 tttattatta atgtgaaaag taaaaggtgt aaaaatgtct tgataatcat tttgcgtttt
     4561 ttgttttgat tgtggttaca aaactaagaa ttgaagcaat tagaaacttt ttattcaaat
     4621 ataaggttta aatcaatgat tagcggtgat attctttaat gctaataaaa atcttattgc
     4681 tatttttaga tgaatactta taaaaattat ttaatgagga aaaaatgaaa aatctaaaat
     4741 tgtttttatt atttatattc aactttgcaa tcttctctca aattgtatta tttgctcagg
     4801 agtccgggaa taaagaaaca atagatccca aaattgttct cgcgaaatat cttgatgcaa
     4861 taggaggaag agatgctctt gctaaagttg aggacagaac tacaatcatg cgcggcacag
     4921 caatgggaca aaatcttacc atcatcgcaa agcaaaaagc tccgaataaa atgcgtcagg
     4981 aagttaaagc cggtggaatg gatcaaacaa ttatttttga cggcgaaaaa ggagtcatga
     5041 aagctgcaac acaaactatt gatattacag ggaaagaatt agagcagctt aaaatagaag
     5101 ctagtatgga attaatgctg gatcccgaaa gttttggcat taagctttcc tacgaaggaa
     5161 ctgaaaaaat tcatgataaa gatgcttaca aaattaaaat gactttacct tcg
//
LOCUS       DASWBY010000729         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_217488, whole genome shotgun sequence.
ACCESSION   DASWBY010000729 DASWBY010000000
VERSION     DASWBY010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217488"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VF870_13220"
     CDS             complement(<1..809)
                     /locus_tag="VF870_13220"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9253199.1"
                     /translation="MYNKLITNIKPLGFTWETKDPFLFCVHHLDNYPAGNDELGPAAS
                     LAGRNLGQDFTTRDGWRMYHGEIVPGFPAHPHRGFETVTVVLNGFVDHSDSHGAAGRY
                     GNGDVQWMTAGSGLQHSEMFPLLNKDKSNTLELFQIWLNLPKVKKFAKPHFKMLWAED
                     IPSVTSKDRNGNSTDITIIAGKLADVSAPPPAPDSWANDSGNEIAIWLIKMEADAEWS
                     IPAASLEVNRSLYIYRGAGINITGITIENYHSVDLLADQPVIITNGNEETSL"
     gene            complement(1055..2176)
                     /locus_tag="VF870_13225"
     CDS             complement(1055..2176)
                     /locus_tag="VF870_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253200.1"
                     /translation="MRKLLFIILFLSSFFANQINAQSLVDTLDNKFDLSQMMASKYGF
                     IAIPFIITEPAIGYGGGAALVFLHKTPEDLAKMGKKTPDLSGVFGMYTESNSWAVGAG
                     HMGFWNDGAIRFRGGAGYLSLNLKYYPELITENSDTELKFGLDGYIIYAETVFRLFDS
                     DFYAGGNYVFSNNKVKFDVPEKVPESIPTEFEKNIGGLGGIINYDSRDNIFTPNNGMS
                     AAVQMVFFDDIFGSDETFQRLFSHWLGFLMLSKNINAGLRLDYQNSFGDMPFYLRPYI
                     SLRGIPAMRYQGKSTYLIETEVRWDWNLRWSLVGFTGYGEAQPINEDISNKQTAYNYG
                     MGFRYLIAREYGIRMGIDIARGPEQWAFYLQFGSSWFSY"
BASE COUNT          758 a          448 c          369 g          764 t
ORIGIN      
        1 agtgatgttt cttcatttcc attagttata ataactggtt gatccgcaag cagatctact
       61 gaatgataat tttctattgt aattcctgtt atgttgatgc cagctcctct gtaaatataa
      121 agtgatctgt ttacttctag cgatgctgca ggaattgacc attcagcatc tgcttccatc
      181 tttatcagcc agattgcaat ttcatttccc gaatcatttg cccatgaatc aggtgctggt
      241 ggaggtgcag atacatcagc caatttaccg gcaataatag ttatatctgt actatttcca
      301 tttctatctt tacttgtaac tgaaggaatg tcctccgccc aaagcatttt aaaatggggc
      361 ttggcaaatt ttttgacttt tgggagattt agccaaattt gaaaaagttc tagtgtatta
      421 gatttatctt tattaagcag gggaaacatt tcagaatgct gcaatccgga accagcagtc
      481 atccactgta catcgccatt gccatatctg ccggctgccc cgtgtgaatc agaatgatcg
      541 acaaaaccat ttagtacaac tgtaactgtt tcaaaacccc tgtgaggatg tgcaggaaaa
      601 ccggggacaa tttctccgtg atacattcgc cagccatctc ttgtagtaaa gtcctgtcca
      661 agatttcttc cagctaatga tgcagctggc cccaattcat cattaccggc aggataatta
      721 tcgaggtgat gcacacaaaa taaaaatgga tccttagtct cccaggtaaa acctaaaggt
      781 ttaatattgg taattagttt gttgtacatt ttaatccttt cattataata attatctaaa
      841 cttcaacttc aatcctatag taatatattc aatttaaaat aattactaaa agataattta
      901 tagtttcaat ctaaataaaa aatttactta ggagtaagga tggattataa aatgctttaa
      961 tcttataatt acattcggtg cgtacggtag attcttatgg aaaattttaa gttgtaaaat
     1021 cgatgctcag atttattgga atattattaa ttcgctagta actaaaccag gaacttccaa
     1081 attgcagata aaaagcccat tgttccggcc cacgcgcaat atcaattccc attcttattc
     1141 catattcgcg tgctattaaa taacggaatc ccattccata attataagca gtttgtttat
     1201 tggaaatatc ttcatttatt ggttgtgctt ctccgtatcc cgtaaaacca acaaggctcc
     1261 atcttaaatt ccaatcccat cttacttccg tttcaatcag gtaggttgat tttccctggt
     1321 atcgcattgc gggaataccg cgcaggctta tgtaaggtct taaatagaaa ggcatatctc
     1381 caaaactgtt ttgataatca agtcttaatc ctgcattaat atttttggat aacattaaga
     1441 atccaagcca gtgactaaat aaccgctgaa atgtttcatc agaaccgaaa atatcatcaa
     1501 agaaaaccat ttgcactgct gcagacattc cattgtttgg agtaaaaata ttatcacggc
     1561 tgtcataatt aattattccg cccaaaccgc caatattctt ttcaaactca gttggaatcg
     1621 attctggtac tttttccggg acatcaaact ttactttatt gttggagaaa acataattac
     1681 ctccggcgta aaaatctgaa tcaaataatc tgaaaactgt ttcagcataa ataatatatc
     1741 catccaaccc aaattttaat tctgtatctg aattttcagt aatcagttca gggtaatatt
     1801 ttaagttaag tgaaaggtaa cctgctccgc ctctgaatct gattgcgcca tcattccaaa
     1861 aacccatatg acctgcacca acagcccagg aattactttc tgtgtacatt ccaaaaacac
     1921 cggataaatc aggagtcttt tttcccattt tagcaaggtc ctcaggtgtt ttgtgaagaa
     1981 atacaagtgc agcaccgcct ccatagccta tagctggctc agttataata aatggaatcg
     2041 caatgaagcc gtatttggag gccatcatct ggcttagatc aaatttatta tctaaagtat
     2101 ctactaagct ttgagcatta atttgatttg caaaaaacga tgacagaaat aaaataataa
     2161 agagtagttt tctcatagcg gaattcaata caaaaattaa tattaattgt atttctaaac
     2221 ttataactcc agttattaaa accaaattta tagatataga tttacataac gataaattca
     2281 attttcaatt tagttactta acaataattt tagattgatc gaaaataaaa agtatttag
//
LOCUS       DASWBY010000730         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_327522, whole genome shotgun sequence.
ACCESSION   DASWBY010000730 DASWBY010000000
VERSION     DASWBY010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327522"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VF870_13230"
     CDS             complement(<1..737)
                     /locus_tag="VF870_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9253201.1"
                     /translation="MSKLSLNDISISIKTELDQFENIFKSSLRSNVGLVDLVARYIIR
                     QKGKKIRPLLVLLSAKISGGVTERTYRGATLVELLHTATLIHDDVVDDANKRRGMWSI
                     NALFKNKVAVLMGDYLLSRGLMISVDGKDYDFLGVTTNAVKRMSEGELLQIQKTRKLD
                     IDEETYFKVISDKTASLLETCCSIGAMSTTDNKDYIEAMRNFGHSLGMSFQIRDDILD
                     YEGKSNLIGKPVGGDIKEKKITLPLIYS"
     gene            complement(734..1495)
                     /gene="tatC"
                     /locus_tag="VF870_13235"
     CDS             complement(734..1495)
                     /gene="tatC"
                     /locus_tag="VF870_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9253202.1"
                     /translation="MAEDILKSDFDEEGIEPEKEMTFLEHLEELRWRIIFSIIGIVVG
                     TIVAWIFIDFLVDVVLLKPAKDSGAHLQNLRPFGQLFLYMQIAIMVGIIISIPNIFYQ
                     FWRFISPALRKHERRYIFWIVAFSSICFLIGIAFAYFVMLPLTLKFAAQFGSESIKNE
                     FSIEEYMSIIISVMLAAGLIFELPMVSFFLSKLGILKPSFMKKFRKHAIVVIMIAAAF
                     LTPGTDPVSQIVLAIPLVVLYEISILVSKISQKKS"
     gene            complement(1508..>1879)
                     /locus_tag="VF870_13240"
     CDS             complement(1508..>1879)
                     /locus_tag="VF870_13240"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560320.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9253203.1"
                     /translation="LSAKLMQYDFNVVSGGTDNHLMLIDLSNKNITGKQAEIALEHAG
                     ITVNKNMVPFDKRSPFVTSGIRVGTPALTTRGMKEAEMEVIASIINKAISNFENEKVL
                     NDLQEEVMKFTKSFPLFAEMK"
BASE COUNT          613 a          360 c          290 g          616 t
ORIGIN      
        1 gaatagatta gcggtaatgt aattttcttt tcctttatat caccgccaac gggtttacca
       61 attaaatttg atttgccttc ataatcaaga atatcatcac gaatctggaa agacattcca
      121 agtgaatgtc caaaattccg catcgcttca atatagtctt tgttatcagt tgtgctcatt
      181 gctcctatcg aacagcaggt ttctaacagt gaagctgttt tatcagagat aactttgaaa
      241 taggtttctt catcaatatc taattttctc gtcttttgga tttgaagaag ttcaccttca
      301 gacattcttt ttacggcatt ggtggtaaca ccaagaaaat cataatcttt gccatcaaca
      361 gaaatcatca atccgcggga aagcaaataa tcacccatca atacagccac tttattttta
      421 aacaaagcgt tgattgacca cattcccctt ctcttatttg catcatcaac aacatcatcg
      481 tgaataagcg tagctgtatg taataattca actaaagttg cacctcgata agttctttct
      541 gtaacaccac ctgaaatttt tgcagaaagc aaaactagaa gtggacggat ttttttacct
      601 ttttgtctaa tgatataacg cgcaacaaga tcaactaatc caacattaga acgcagtgat
      661 gatttgaaaa tattttcaaa ctgatcaagt tctgttttga tagaaatact tatgtcattt
      721 agtgaaagtt tactcacgat ttcttttgcg atattttaga aactaaaata cttatttcat
      781 atagcacaac caacggaatg gctaacacaa tctgggatac gggatctgtt cccggcgtta
      841 aaaatgcggc agcaatcatt attacgacaa tagcatgctt acggaatttt ttcataaacg
      901 atggtttaag tattccgagt ttggataaaa agaaagagac cattggcagt tcaaatatta
      961 atcctgctgc aagcattacg ctaataatga ttgacatata ctcttctatc gaaaactcat
     1021 tcttaattga ctccgaaccg aattgtgccg caaattttaa tgttaaggga agcattacaa
     1081 aatatgcgaa agctattcca attaaaaaac agattgatga aaatgcaaca atccaaaata
     1141 tgtatcgccg ttcgtgtttg cgcagtgctg gtgaaataaa ccgccagaac tgataaaaaa
     1201 tattcgggat gcttataatt ataccaacca taattgcaat ttgcatgtaa agaaaaagct
     1261 gtccgaacgg tctgaggttt tgtagatggg caccagaatc tttagcaggt tttagcagta
     1321 ctacatcaac aagaaaatct ataaatatcc aggcaacgat agttccaaca acaataccaa
     1381 ttattgaaaa tataattctc catcttaatt cctcaagatg ctcaagaaaa gtcatttctt
     1441 tttcaggttc aataccctct tcatcaaaat cagattttag tatatcttct gccactatag
     1501 ctttctatta cttcatttct gcaaataatg ggaatgattt tgtaaacttc ataacttctt
     1561 cctgaagatc attcaatacc ttttcatttt caaaattaga gattgcttta ttaataattg
     1621 aagctatcac ttccatttct gcttctttca ttccgcgtgt tgtaagtgcg ggcgttccaa
     1681 ctcttattcc gctagttaca aacgggctgc gtttatcaaa aggaaccata tttttattaa
     1741 ctgtaattcc ggcatgttct agtgcaatct ctgcctgctt accggtaata tttttatttg
     1801 aaagatcaat taacattaaa tggttatcag ttccgcctga tacaacatta aaatcgtact
     1861 gcattaattt tgcggataa
//
LOCUS       DASWBY010000731         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_105546, whole genome shotgun sequence.
ACCESSION   DASWBY010000731 DASWBY010000000
VERSION     DASWBY010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105546"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VF870_13245"
     CDS             <1..378
                     /locus_tag="VF870_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HEX9253204.1"
                     /translation="LTQTNIDEIEEQGFYEIKDGEMMINSVNNHDCVFSFWDNGIAKC
                     AIERAYFDGKVEFRKPISCHLFPIRVSDFSGDVLRYEKFSECAPALEKGKADNVTIAE
                     FCKDSLTRLYGEEWYNKLMNYIR"
     gene            384..1838
                     /gene="rpoN"
                     /locus_tag="VF870_13250"
     CDS             384..1838
                     /gene="rpoN"
                     /locus_tag="VF870_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX9253205.1"
                     /translation="MLSLSQRLTQSQKLSPQQIQYQKLLQLNTLALEQRIKTELEMNP
                     VLEEEMEMTLDQDDKEKDSDSETEKEDEFSDKDNEEFDLEDYSNDDDFDHEKINRSAD
                     EEIYQPLAPQRESLSEHLTEQLRLLNLEENLFILGEEIIGNLDEDGYLKRDLAEILNE
                     LDMFEHIKIDAQTGEDLLKRIQKFDPIGIACRSLQECLIVQLRESKSDPYIKFLAIKL
                     LEDHYDEFTKRRFDLIKHRMNFTDESLRDTVALIQSMNPKPGEGNISSMESNQVTPDF
                     LVEKVDENWIITLNDRSMPSVTISKHYLEMIDSNKRGKKNPREKETYKFLREKFESAK
                     WFIACIQQRRETLMKIMRAILERQYEFFEKGPKLLKPMIYKDIAEEIQMDISTISRVV
                     NGKYVQSPMGIHELKYFFSEGLSTDSGEEVSNKHIRERLKEIIEAENKKAPHSDDKLA
                     ELLNGEGIHIARRTVAKYREQLKLPVARLRKQMM"
     gene            1839..2456
                     /locus_tag="VF870_13255"
     CDS             1839..2456
                     /locus_tag="VF870_13255"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9253206.1"
                     /translation="MNYVIDVIVLVLLFSIYGFIHSILASQTVKLLFKKTFGKLIAFY
                     RLIFNVFSVVGLYLIWDLAPHPSLQIYKLPQPYDYLVLIPQLISLAGIIWCFKYVCFK
                     EFLGLTQIDRYLKNEYSDDELDENYTLRIEGPYKFSRHPIYFFSIIFLLFRAEMDLFY
                     LTMFISFTAYFYFGSYYEEKKMVRLFGDDYRNYQQKVPKIFPIKI"
     gene            2544..>2817
                     /locus_tag="VF870_13260"
     CDS             2544..>2817
                     /locus_tag="VF870_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEX9253207.1"
                     /translation="MKQKLRSTTILGLVHNGVAALGGDGQVSLGNTVMKHNAMKIRKI
                     ANGKVLCGFAGASADAFTLMERFEDKLEQYRGNVNRAAVELAKDWRT"
BASE COUNT          999 a          414 c          542 g          862 t
ORIGIN      
        1 ctgacacaga ctaatattga tgaaattgag gaacaaggat tctatgaaat taaagacggc
       61 gaaatgatga ttaatagtgt aaataatcat gattgcgttt tttctttttg ggataatggg
      121 attgcaaagt gcgcaattga acgcgcatat tttgatggca aagttgaatt taggaaaccg
      181 atttcctgtc atctttttcc gattagagtt tccgatttta gcggcgatgt tttacgatat
      241 gaaaaattca gcgaatgtgc accggcatta gaaaaaggta aagcggataa tgttactatc
      301 gcagaatttt gtaaagattc tttaacaaga ttatacgggg aagaatggta caataaactt
      361 atgaactata tcaggtagac aatatgcttt cactcagcca aagacttaca caaagccaaa
      421 agctttcacc gcagcagatt caatatcaaa aacttctgca gttaaatact cttgctctgg
      481 agcagagaat taaaactgaa ttagaaatga atcctgttct tgaagaagaa atggaaatga
      541 ctttggatca ggatgataag gagaaagact ccgattcgga aacagaaaaa gaagatgaat
      601 tttctgataa agataatgag gaatttgatc ttgaagatta ttctaatgat gatgattttg
      661 atcatgaaaa aataaaccgc agtgcagatg aagaaattta tcagcctctt gcaccgcaaa
      721 gagaatcact tagcgaacat ttaaccgagc agttaagatt attaaacctt gaagaaaatc
      781 ttttcattct tggtgaagaa ataatcggta atcttgatga agacggatat ttaaaacgtg
      841 atcttgcgga gatattaaac gaacttgata tgtttgaaca tattaaaata gatgctcaaa
      901 caggggaaga tcttttaaag cgaattcaaa aatttgatcc gattggtatt gcttgcagaa
      961 gtttacagga atgtttaata gtgcagcttc gtgaaagcaa atccgatcca tatataaaat
     1021 ttcttgctat caagctgctt gaagatcatt acgatgagtt tacaaagaga cgttttgatc
     1081 taataaaaca tagaatgaat tttaccgacg aaagtttacg agataccgtt gctctgattc
     1141 aaagtatgaa tcccaaacca ggcgaaggaa atatttcttc gatggaaagc aatcaggtaa
     1201 cacctgattt tcttgttgaa aaagttgatg aaaactggat aataacttta aatgacagaa
     1261 gtatgccttc agttactatc agtaagcatt atctggaaat gattgattca aataaacgtg
     1321 ggaagaaaaa tccacgcgag aaagaaacat ataaattttt aagagaaaaa tttgaatcag
     1381 caaagtggtt tattgcatgt atacagcagc gccgggaaac gctgatgaaa attatgcgtg
     1441 ccattttaga aagacaatat gagttttttg aaaaaggtcc aaaactccta aagccgatga
     1501 tttacaaaga cattgctgaa gaaattcaaa tggatatatc gacaataagc cgtgttgtaa
     1561 acggtaagta tgttcaaagt ccaatgggca ttcacgaatt aaaatatttc ttcagcgaag
     1621 gattatctac agattccggt gaagaggttt ccaacaagca cattcgtgaa agacttaaag
     1681 aaataatcga agctgaaaat aaaaaagcac ctcacagcga tgataaactt gcagaacttt
     1741 taaatggtga aggtattcac attgcaagac gaacggttgc aaaatatcgt gaacaactaa
     1801 aacttcctgt ggctcgtttg cgtaaacaaa tgatgtaaat gaattatgtt attgatgtaa
     1861 tagtcttggt tctccttttt tcgatctatg gatttatcca ttcaattctt gcatctcaaa
     1921 cagttaagtt gcttttcaaa aaaacttttg gcaagctaat agctttttat cgtttaatat
     1981 ttaatgtgtt ttctgttgta gggttatatc ttatatggga tttagcccct catccgtctt
     2041 tacaaattta taaacttcct cagccgtatg attatcttgt attgattcca caattaatat
     2101 ctcttgctgg aataatctgg tgcttcaagt acgtgtgttt caaggaattt ttaggattga
     2161 ctcaaattga tcgttattta aagaatgaat attccgatga tgagttggat gaaaattata
     2221 ctttacgaat tgaaggacca tacaaattca gccgtcatcc aatttatttt ttctcaataa
     2281 tatttttatt gttcagagca gagatggact tgttttatct cacaatgttc atatcattta
     2341 cggcatattt ttactttggt tcatactatg aggaaaagaa aatggttaga ttgtttggtg
     2401 atgattacag aaattatcag cagaaagtac caaaaatttt tccaattaaa atttaaatgt
     2461 tatgtggaat tgtcatccct gctaaatcag ggatctattt ttttaagtaa ttaaaagaag
     2521 attatgaaac gaaagaaatt attatgaaac aaaaattaag atcaacaaca atacttggat
     2581 tagttcacaa cggagttgca gcactcggtg gtgatgggca ggtatcgctc ggcaacacag
     2641 ttatgaaaca taatgcaatg aagataagaa aaattgcaaa tggaaaagta ctttgcggtt
     2701 ttgcaggtgc ttccgctgat gcatttacac ttatggaaag atttgaagat aaacttgaac
     2761 aatatcgcgg caatgttaat cgtgccgctg tagagctcgc aaaagattgg cgcacag
//
LOCUS       DASWBY010000732         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_66625, whole genome shotgun sequence.
ACCESSION   DASWBY010000732 DASWBY010000000
VERSION     DASWBY010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66625"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="hutI"
                     /locus_tag="VF870_13265"
     CDS             <1..798
                     /gene="hutI"
                     /locus_tag="VF870_13265"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX9253208.1"
                     /translation="GYGLDFENEKKLLQAIKVINEIFPINIVSTFLGAHTFPPEFKDN
                     HSGYVELIINKMIPHIAKNNLAEFCDGFCESTAFNSEEIDQIFIAASNAGLKLKLHTE
                     QFNNVGGLDIALKHNAISVDHLEVLLEKDFHKFLNSETVSVLLPGVSLFLDYQFAPAR
                     KLIDNNAIIALATDYNPGSSPILNLHLIMSLAAIKMKMKIEEIINAVTINAAKALNRE
                     KNVGSIEIGKKADFAVFNTNDYSDIVYNVGRNILSHTIKDGEIIFQK"
     gene            complement(904..>1536)
                     /locus_tag="VF870_13270"
     CDS             complement(904..>1536)
                     /locus_tag="VF870_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9253209.1"
                     /translation="DTVAMKILKTEPAMNSSTDFKNPEILLSFDDAINNKEIKNALQF
                     TDTSKNKIDFNLSFVDDATISIKPNIDLKPEKNYLLKIDLKYFTDAADNKVDSIYTLK
                     FKTITGVEFTGISGKVLTTKNNVVVVLQDSKDANKFFTTVPDKTSAYSFERIDASTYS
                     MWVYSDKDSSGTFDKGYPDPFRFAEEFYMLPDTIQLRPRWSVTDYNIEFK"
BASE COUNT          528 a          243 c          246 g          519 t
ORIGIN      
        1 ggctacggtt tggattttga gaatgagaag aaactgctgc aagcgattaa agtgattaac
       61 gaaattttcc caataaatat tgtttcaact tttcttgggg cacatacttt tccccctgaa
      121 tttaaagaca atcattcagg ctatgttgaa ttaattatta ataagatgat tccgcatatc
      181 gctaaaaata atcttgcaga attttgtgat ggattttgtg aatcgacagc ttttaattct
      241 gaggaaatag atcaaatatt tattgctgct tcaaatgccg gattaaaact taaacttcac
      301 acagagcagt ttaataatgt tggcggattg gatattgcct taaaacacaa cgcaataagt
      361 gttgatcatt tagaagtatt acttgaaaaa gattttcata aatttttaaa ttcagaaaca
      421 gtctctgtgc ttcttcccgg cgtttcgctt tttctggatt atcaatttgc ccctgcaaga
      481 aaacttattg ataataatgc tatcattgca cttgctacag attacaatcc aggatcatca
      541 cctattttaa atctgcatct gataatgagt ttggcggcaa tcaaaatgaa aatgaagatt
      601 gaggaaataa taaatgctgt aacaattaat gcggcaaaag ctcttaaccg tgaaaaaaat
      661 gttggaagta ttgaaattgg taagaaagct gatttcgcgg ttttcaatac aaatgattat
      721 tcagatattg tttataatgt tgggaggaat atactttcac acacaataaa agatggtgag
      781 ataatttttc aaaaatgaag gcacttgttc gttacctcaa gcaactcaaa gtgatccgct
      841 taattattaa aaaataataa tgaattctta ataataaatt tctaataatc gctgcacaag
      901 gaattactta aactcaatat tataatcagt aacactccag cggggacgaa gttgtatcgt
      961 atcaggcagc atataaaatt cttctgcaaa tctgaaagga tctgggtaac ctttatcaaa
     1021 cgtaccactg ctatccttat cagaatagac ccacatagaa tatgtacttg catctattct
     1081 ctcaaatgaa tatgcggacg ttttatcggg tactgtggta aaaaacttgt tggcatcctt
     1141 cgaatcctgc aaaacaacaa cgacattatt cttggtagtt aatactttac ctgaaattcc
     1201 ggtaaactca acacctgtaa tcgttttaaa tttaagagtg taaattgaat caactttatt
     1261 atcagcagca tcagtaaaat attttaaatc tattttcaat agataatttt tttcaggttt
     1321 taaatcgata ttaggtttta ttgatatagt tgcatcatca acaaaactta aattaaaatc
     1381 tattttattt tttgaagtat cagtaaactg tagagcattt ttaatctcct tattatttat
     1441 cgcatcatca aatgataata gtatctcagg attcttaaaa tcagtggagc tgttcatggc
     1501 cggttcagtt tttaaaatct tcatggcaac agtatc
//
LOCUS       DASWBY010000733         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_181346, whole genome shotgun sequence.
ACCESSION   DASWBY010000733 DASWBY010000000
VERSION     DASWBY010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181346"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1772
                     /locus_tag="VF870_13275"
     CDS             <1..>1772
                     /locus_tag="VF870_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9253210.1"
                     /translation="RYAYSQNVVLIASAGNSGSSAPHYPSGYSEVICVGNSTDQDYVA
                     GSSNFGSTIDLVAPGSLIMTSSKNSGYSVISGTSASAPFVSAAASLILSKQSFTNEEI
                     KQILKSTSDDIGEAGWDLKSGAGRLNILKALSVTAPSIIKFHTPAQDYATSGNNISIV
                     ASVLSPYFTNYSLQVGTGFNPTTWLPLITNSLNQISNQEIYNLDVTGYSDSVYTLRLI
                     LNQTTGRTLEERINFHVDRSTPVTELISLIPAFYGNKATVLASMYTDDPSIVKMYYRI
                     YGDTQFNFVTLDGFTINNQFVKSLHYGFIPKDLVTQNTLYEIYFEAENLVGLKTQVKN
                     GSEYFIVPTTFNAELSTEYKLPYSLPAGSIFERSIALSGNNSDEIFLRTNANPKVTSI
                     YNLQNETFSLIDSLNEKIVKDAGDYNNNGLTDLLDYFVRDGFIDEQTSQNSSTFTQKY
                     SNTGGDFWPILASDVDGDGIVEVFSVQNDTTVEVWEVVQPNLDLNKVATLKNFSPSGF
                     GGNIINSPNAVVDDIDGDGVKEFWMIDQDGDIYSYKIKGNNQLVQQYVIPTQFLGSSA
                     YLTSGDFNGDGRRELAVLLHSIED"
BASE COUNT          558 a          300 c          319 g          595 t
ORIGIN      
        1 cgttatgctt actcacagaa tgttgttttg attgcaagtg ccggtaactc tggttcaagt
       61 gctccgcatt atccttctgg ttattctgaa gttatttgcg ttggtaattc aacagatcag
      121 gattatgtag caggaagttc aaacttcggt tcaactattg atttagttgc tccaggttcg
      181 cttataatga catcgtctaa aaattcagga tattctgtta ttagcggaac atctgcatcg
      241 gcacctttcg tttctgcagc agccagttta attttatcaa aacaaagttt tactaatgaa
      301 gagattaagc agattttaaa atctacttca gatgatattg gtgaagccgg ttgggattta
      361 aaaagtggag caggcaggtt aaatattctt aaagcacttt ctgttaccgc accatcaata
      421 attaaatttc atacacccgc tcaggattat gcaacatccg gaaataatat ctctattgtt
      481 gcctcagtgc tatctcctta ctttacaaat tatagtttac aagtcggaac cggttttaat
      541 ccgacaacct ggcttccact tattacaaat agtctcaatc aaatatcgaa tcaggaaatt
      601 tacaacttag atgttacagg ttattcagat tcagtataca ctttaagact gatcctcaat
      661 caaactacgg gaagaactct tgaggagcgt attaattttc atgtggatcg ctctacacct
      721 gttaccgaat taatctcatt aattccggca ttctacggaa acaaggcaac tgttcttgca
      781 tctatgtata cagatgaccc ttctattgta aaaatgtatt accgtatcta tggtgatact
      841 cagtttaatt ttgtgactct tgatgggttt acgattaata atcagtttgt aaaatctttg
      901 cattatggtt tcattccaaa agatctggtt acccaaaata ctttgtacga aatttatttt
      961 gaggcagaaa atcttgttgg attaaaaact caggtaaaaa atggcagcga atattttatt
     1021 gttccaacta cattcaatgc tgaattatcc accgaataca agttacctta ttctttacct
     1081 gcaggaagta ttttcgaaag atcaattgct ttaagtggaa ataattctga tgaaatattt
     1141 cttagaacaa atgctaaccc aaaagtaact tctatatata atcttcaaaa cgaaaccttt
     1201 tcacttattg attcattaaa tgaaaaaatt gtaaaagatg ccggtgatta taataataat
     1261 ggattgacag atctgttaga ttattttgtt cgtgatgggt ttattgatga acagacctcc
     1321 caaaactcct caacatttac tcaaaaatat tcgaacactg gtggagattt ttggccaatt
     1381 ttagcaagtg atgtagatgg tgatggaatc gttgaagttt tttctgttca aaatgatact
     1441 acagttgaag tttgggaagt tgttcaacca aatcttgacc ttaataaggt tgccacactt
     1501 aaaaatttta gtcctagcgg ttttggagga aacataatca actcacctaa tgcagttgtt
     1561 gacgatattg atggagatgg cgtaaaagaa ttctggatga ttgaccagga cggtgatatt
     1621 tacagctata agattaaagg taataatcag ttggtacagc aatatgtaat tccaacacag
     1681 tttcttggtt cttcagctta tttaaccagc ggggatttta atggagatgg cagaagggaa
     1741 cttgcggtct tactacattc aattgaagat tt
//
LOCUS       DASWBY010000734         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_517907, whole genome shotgun sequence.
ACCESSION   DASWBY010000734 DASWBY010000000
VERSION     DASWBY010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517907"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1041)
                     /gene="lipA"
                     /locus_tag="VF870_13280"
     CDS             complement(127..1041)
                     /gene="lipA"
                     /locus_tag="VF870_13280"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX9253211.1"
                     /translation="MINQHQRAFKEEAEKTNKELGKRPDWLKVRLPIGENYSDVKNLM
                     RRQKLNTVCEEAKCPNISECWNHRTATFMILGDTCTRSCGFCNIKVGIPNELDLEEPK
                     RVVESVVELKLRHVVITSVNRDELKDGGATIFKECVRLIREQKPDCTIEILIPDFKGD
                     EESFKIIMQNPPDILNHNLETIQRLYHAVRPQAKYERSLELIKWFKSKGLKTKSGIMV
                     GIGEKTEEVLELMKDLVSHGCDILTIGQYLQPTKQHLPVDRFVTPDEFNMYKEEGLKF
                     GFKIVESSPLVRSSYHADEHARAIFKDN"
     gene            complement(1146..2870)
                     /gene="sucB"
                     /locus_tag="VF870_13285"
     CDS             complement(1146..2870)
                     /gene="sucB"
                     /locus_tag="VF870_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEX9253212.1"
                     /translation="MDIVMPKMGESVNEGTIIKWHKKVGDVIKLDEVIFEISTDKVDT
                     EIPAATAGILSEILVKEGETVEVGTVVARISSDEKSNNENVISKSDAINQSDESAQTA
                     LNKIESSEQLNNDQSPNSEVASKPIISGNVLEIAMPKMGESVMEGTIIKWHKKVGEAV
                     KKDETIFEISTDKVDTEIPSPEAGTLAEILVGEQETVDVGTIVAKLSVGNDVKVSQPA
                     GQEKVGDQVNVQEEKSMHDNFSNIDIQPKEQGTGSGFYSPLVLSIAQKENISFDELKS
                     IQGTGLEGRVSKKDVLAYIEKRKMGKVSQDYKQPQYQVQTKQEIKSASPAPAFVPTTS
                     QTVYNEAEVERIPMDNIRQRIMDHMVLSRDTSVHVTELIEVDMTRIHNFIAAKKDEIM
                     KNENAKITYMSFISDAVVKSLKKYPLVNSSIDGNTILQKKFINLGIAVAIEPTGLIVP
                     NIKGAGDRNVIGLAKAIADLANRSRTKKLTPDDISNGTFTITNYGVFGTIFGTPIINQ
                     PEVAILGVGAVQKKPVVIEVDGSDTFAVRHIMALTLSHDHRLVDGMLGGLFLKNIKEI
                     LENFEGII"
     gene            complement(3000..>3476)
                     /locus_tag="VF870_13290"
     CDS             complement(3000..>3476)
                     /locus_tag="VF870_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9253213.1"
                     /translation="CEHKGLYRQSSAISPEPDENYLLPFGKANVIKEGTDVTVVSWGV
                     SLWDSMLAAKKLEDEDFSVELIDLRTIVPLDEETIYNSVKKTNKVIIIHEDTLFGGFG
                     AEIAARISDNCFQYLDAPVKRIAAKDSHIPYAPILENEVLPSRADIYKGIRDLLKY"
BASE COUNT         1013 a          673 c          522 g         1268 t
ORIGIN      
        1 aaaggaagtg gtttttaatt tcatttagta aaaatattcg gtaataaact tctgttaatt
       61 tttgagtggt taaaataagt agattattgt taataagaaa tgaaaatcta tataaaatat
      121 ttgttattaa ttgtctttaa atattgctcg tgcatgttca tcagcgtgat atgaactgcg
      181 aacaagtggt gaagattcaa cgatcttaaa tccaaatttt aacccttctt ctttatacat
      241 attaaattcg tccggagtta caaatctatc aacgggtaaa tgttgttttg tcggctgcaa
      301 atattgtccg attgttaaaa tatcgcaacc gtgggatact aaatctttca taagttctaa
      361 aacttcttcg gttttttcac caatgccgac cattattccg ctcttggttt taagtccctt
      421 tgatttaaac catttaatta gttccaagct tctttcatac tttgcttgcg gacgaactgc
      481 gtgataaagt ctttggatcg tttccaaatt atgattaagt atatccggcg gattctgcat
      541 gataatttta aacgattctt catcaccttt aaaatccgga attagaattt caatggtaca
      601 atcaggtttt tgttcacgaa tcaatctaac acattcttta aatattgttg ctccgccgtc
      661 ttttaattca tctctattta cagatgtaat tacaacgtgc ctcagcttta gttcaacaac
      721 agattcaaca acgcgttttg gctcttctaa atcaagttca tttggaattc caacttttat
      781 attgcaaaag ccacagctgc gagtacaagt atcgcccaga atcataaacg ttgcagtacg
      841 atgattccag cattcagaaa tatttgggca tttagcttct tcgcaaacgg tatttagttt
      901 ttgtctgcgc ataagatttt taacatcgga gtaattttct cctattggta atcttacttt
      961 aagccaatct ggtcttttgc ctaattcttt attcgttttt tcagcttctt ctttaaatgc
     1021 acgttgatgt tgatttatca taatacaatc tataaaaatc gtcacactga gcgaagtcga
     1081 agtgtgacat aataaatgtg aggtcatctt tcgaatttgc tcaagatgac tttcgcaagg
     1141 attacctaaa taatcccttc aaaattttct aatatttctt taatattctt taggaacaat
     1201 ccacccagca taccatcaac caaacgatga tcgtgtgaga gtgtcaatgc cattatgtgt
     1261 ctgacggcga aggtatcgga accatccact tcaattacaa ctggtttttt ctgaacggca
     1321 ccaactccaa gtattgcaac ttcaggttga tttataattg gcgttccaaa tatagttccg
     1381 aacacaccat aatttgtaat tgtaaacgtt ccgttactta tatcatcagg agtaagtttt
     1441 ttagtcctgg agcgatttgc aagatcagcg attgcttttg caagcccaat tacatttcta
     1501 tcacctgcac cttttatgtt cggtacaatc aatcctgtcg gttcaatcgc aacagctata
     1561 ccaagattga taaatttctt ttgcaggatt gtattaccat ctatgctgga atttacaagc
     1621 ggatatttct ttaacgattt cacaactgca tcagaaataa atgacatata agtgatctta
     1681 gcattttcat ttttcataat ctcatctttt tttgccgcga tgaaattatg aattcttgtc
     1741 atatcaactt caataagctc agtaacgtga acagaggtat cacgactaag aaccatgtgg
     1801 tccataattc tttgacgaat attatccatt gggattcttt caacttctgc ttcgttatag
     1861 actgtctgtg aggttgttgg aacaaaagct ggggcaggag aagcagattt aatctcttgt
     1921 ttagtttgaa cttgatactg aggctgcttg taatcttggg aaactttgcc cattttcctt
     1981 ttttctatat aagcaagaac atctttttta gagactcttc cttccagacc agtgccttga
     2041 atcgatttca attcatcaaa acttatattt tctttttgtg cgatacttag aacaagcgga
     2101 gaataaaatc ctgaacctgt accctgttct tttggttgga tatctatatt tgaaaaatta
     2161 tcatgcatag atttttcttc ttgaacatta acctgatcgc caactttttc ctgtccggct
     2221 ggttgagaaa ctttaacatc atttccaaca gaaagctttg caacaatagt tcctacatca
     2281 actgtttcct gttcaccaac taatatctct gctaatgtac ctgcttctgg tgaaggaatt
     2341 tctgtatcaa ctttatcggt actgatttca aaaattgttt catctttctt tactgcttca
     2401 ccaacttttt tatgccactt aataattgtt ccttccatta ctgattcgcc cattttgggc
     2461 attgcaattt caagaacatt tccagaaata attggctttg aagccacttc tgagtttggt
     2521 gattgatcat tgttaagttg ctcagaagat tcaatcttgt ttaatgcagt ttgtgcagat
     2581 tcatcagact gattaattgc atcagacttt gaaataacat tttcattatt agatttttca
     2641 tctgagctta ttcttgccac aacagttccg acttcaacag tttctccttc cttgaccaat
     2701 atttcagaaa gaattcccgc tgttgctgcc ggaatctctg tatcaacttt atcagtactt
     2761 atttcaaaaa ttacttcatc aagttttata acgtcgccga cttttttatg ccactttatg
     2821 attgtgcctt catttacact ttcacccatt tttggcatta ctatatccat attaaatcct
     2881 ctttacaaat gtttatttct aaatttcaat tatcaattat tgtgtcatat cgagcgaatc
     2941 gagatatctg taacttaata caaatcaata tacttcttac tgatcgataa aacgactttt
     3001 tagtacttaa gtaaatctct tattcctttg taaatatcag cacgactcgg aagaacttca
     3061 ttctctaaaa taggtgcgta tggaatatgt gaatctttgg ctgcaattct ttttaccggt
     3121 gcatcaagat attgaaaaca gttatcagaa atacgggctg ctatctccgc gccaaatcca
     3181 ccaaacaatg tatcttcatg aattattata accttatttg ttttctttac tgagttataa
     3241 attgtttctt catctaaagg tacaatcgtc cttaaatcta ttaattcaac tgaaaaatct
     3301 tcatcctcta attttttagc cgccagcata gaatcccata aggaaactcc ccatgaaaca
     3361 acagtaacat cagtaccctc ttttataaca ttagctttac cgaatggtaa taaataattt
     3421 tcatcgggtt caggactaat tgcagagctt tgtctgtata atcctttatg ttcaca
//
LOCUS       DASWBY010000735         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_327862, whole genome shotgun sequence.
ACCESSION   DASWBY010000735 DASWBY010000000
VERSION     DASWBY010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327862"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF870_13295"
     CDS             complement(<1..189)
                     /locus_tag="VF870_13295"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX9253214.1"
                     /translation="MKLLLILLFAISLIQTSSFTQSKEETKTLLIRCDDLGMSHAVNA
                     AFKELMESGLKFSASVMFP"
     gene            complement(186..920)
                     /gene="tsaA"
                     /locus_tag="VF870_13300"
     CDS             complement(186..920)
                     /gene="tsaA"
                     /locus_tag="VF870_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX9253215.1"
                     /translation="MNNEILLSPIGNVKSHLKHRYETPRQGVLAKQSKAIIYLNPKNN
                     FEQAVKDLDGFERIWIIYQFHLNKNWKPLVTPPRHTRNKVGVFATRAPYRPNQIGLSC
                     VKLDKIEGLKIFISESDLLDDTPVIDIKPYLPYSDSFPGVKTGWAKSDLSEMYNVTFN
                     SKARIAVEELKFEKDINLFDYARIQLEFNPTDLSRKRISNTNPKKSGGEIFTLSYQDW
                     QIHYVVDELKKKVKITNIIFVDRKKL"
     gene            complement(1089..>1558)
                     /locus_tag="VF870_13305"
     CDS             complement(1089..>1558)
                     /locus_tag="VF870_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9253216.1"
                     /translation="CVHAMKLFMQNENINTLILVSGDRDFIPLLNELRSLGKNTYLMG
                     VPGSISRDLVNVADGIIEYRPEKIKQQESKSLQTPQKKVSDQIRDRVLAMLKKTNRRV
                     NVATIGIMIKKEHPAFNHKEFGYEKLSQFLDGFPQIKIEYDEDKLVAYASLIK"
BASE COUNT          486 a          281 c          241 g          550 t
ORIGIN      
        1 aggaaacata actgaagcag aaaattttaa tccggattcc attaattctt taaaggctgc
       61 attaacagca tgagacattc caagatcatc acatctgata agtaacgttt ttgtttcttc
      121 ctttgattga gtaaatgatg aagtttggat caaagaaatc gcaaacaaaa ggataagtaa
      181 cagtttcata attttttcct atcaacaaaa atgatattag taattttaac tttctttttt
      241 aattcatcaa ccacataatg gatttgccaa tcctggtaac ttaacgtaaa aatttctccg
      301 cctgattttt taggattagt attacttatt cttttcctcg ataaatcagt tggattaaat
      361 tcaagctgga tccgtgcata atcaaaaaga ttaatgtctt tttcaaattt taactcttca
      421 acagcaatcc tagccttaga gttaaaagta acattgtaca tttcagaaag atcacttttt
      481 gcccaaccgg ttttaacacc aggaaaagaa tctgaataag gtaagtatgg tttgatatca
      541 attaccgggg tgtcatcaag aagatcagat tctgaaataa atatttttaa tccttctatt
      601 ttatcaagtt taacacaact taatccaatc tgattcggtc tgtatggtgc acgagttgca
      661 aacacgccaa ctttattcct ggtatgcctt ggcggagtaa caaggggttt ccagttttta
      721 ttaagatgga actgatagat gatccatatt ctttcaaagc catccagatc ttttacagct
      781 tgctcaaaat tatttttggg atttagataa ataatcgctt tactttgctt tgcaagaact
      841 ccctgtcttg gtgtttcata tcggtgttta agatgagatt taacattacc aatcggactc
      901 aataatattt cattattcat agttaaaatt aaatctaata tcaggttagt tcaattgatt
      961 gaagaaataa aaaaaattaa tgtcgtgatt cactatcagt cctggttccg gaaaattaaa
     1021 ttcttaagtc tgtttttatt aaaacttgat tcttgcttag attcttattt ttttctgtct
     1081 gacacttttc atttaattaa actagcataa gcaaccagct tatcttcatc atactcaatt
     1141 ttaatctgtg ggaaaccatc aagaaactgg gaaagttttt cgtaaccaaa ttctttgtga
     1201 ttgaatgccg gatgctcctt cttaatcata atcccgattg ttgcaacatt tactcttcga
     1261 ttcgttttct ttaacatagc aagcaccctg tcccgtattt gatcactcac cttcttctgt
     1321 ggagtctgca gagactttga ttcctgttgc ttaatttttt ccggacggta ctctataatt
     1381 ccatctgcta catttacaag atccctggat attgaaccgg ggacacccat taagtatgtg
     1441 tttttcccga gtgacctgag ctcattaaga agcgggataa aatcacgatc acctgaaacc
     1501 agtattaaag tattaatgtt ttcattctgc ataaacagtt tcatagcgtg aacgcaga
//
LOCUS       DASWBY010000736         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_66884, whole genome shotgun sequence.
ACCESSION   DASWBY010000736 DASWBY010000000
VERSION     DASWBY010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66884"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VF870_13310"
     CDS             <1..796
                     /locus_tag="VF870_13310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9253217.1"
                     /translation="GLLPGADRDLVNYLSIRIKKSFAEIMTNSKVLEMKEVKDGISVK
                     IQDDKGNVADKVYDYVLMSIGRKPETRGLGLENTKVTVTDRGWIKVDKQLRTIDPNIF
                     AIGDIAGEPMLAHKASHEGRVAVEVIAGHKAYFEPLAIPAVVFTDPEIAWAGLTEEQA
                     KKEGIKHEVAKFPWAASGRAVTLDRNDGVTKLIIDPDTQRILGIGICGPGAGELIAEG
                     VLAIEMGANVTDLKLTIHPHPTLSETIMDAAEVFFGQSSHFYKPKR"
     gene            complement(873..>2008)
                     /locus_tag="VF870_13315"
     CDS             complement(873..>2008)
                     /locus_tag="VF870_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253218.1"
                     /translation="IKKDQVTVHLGMAISKLASSEPTLSDEAVLKEKIAKVVPELQPS
                     TEVRNGIFSYIMDVKLREQKEQEKIAQALSTEEKQEKDEEEIPIEKEKYVEEEKVKSK
                     KKFKFNPQLFKKLGFTVLALIAIIIVYTILTSPSSGWEVISISGQPKVDNSPMNKNDS
                     FIAETTIETDENSSVTVAIPMMGRLLIDNSSIVSRTKNSNEISLEKGQIRKFEGDASE
                     VFTVLTPLAKFTELYKGGAFRLNVDENGNCKLAVESGWVIVNIKEFDSYVPKNFGCLI
                     SRGRYAIPYPSDSSPELISLLENFSGVNDASIGTILSLITKKESLSLWHLLQLVSSEN
                     RFIVFDRLNEFVPIPSGVTKEGIQALNKDMLLQWRQEIELKMD"
BASE COUNT          620 a          369 c          370 g          649 t
ORIGIN      
        1 tggactttta cccggagccg atcgtgatct tgtaaattat ctttcaataa ggattaagaa
       61 atctttcgct gaaattatga caaatagcaa agtgcttgaa atgaaagaag taaaagatgg
      121 tattagtgtt aagatacagg atgataaagg taatgttgca gataaagttt atgattatgt
      181 gttaatgtca atcggaagaa aacctgaaac gcgcggacta ggattagaga atacaaaagt
      241 tactgttact gatcgcggct ggataaaagt tgacaaacag ctcagaacaa ttgatccaaa
      301 tatttttgct attggtgata ttgccggaga accaatgcta gctcataaag cttcacacga
      361 aggcagagtt gctgttgaag ttattgccgg gcataaagcc tattttgaac cacttgcaat
      421 tcccgctgtt gtatttaccg atccggaaat tgcctgggcg ggattaacag aggaacaagc
      481 gaagaaagag ggaataaaac acgaagttgc aaaatttcca tgggcagcaa gcggcagggc
      541 agttactctt gatagaaacg acggtgtaac aaaactgatt atcgatcccg atacacaaag
      601 aatacttgga atcggaattt gcggtcccgg tgcaggcgaa ttgattgcag aaggtgtgct
      661 tgcaattgaa atgggtgcta atgttacaga cttaaaactt acaatacatc cacatccaac
      721 tttatctgaa acgattatgg atgcggcaga agttttcttt ggacaaagtt cgcattttta
      781 taagccgaag agataatagt tgattagtag ttacaaaaaa cccagtttct taaaggaact
      841 gggtttttag ttttaaagat tttgttaaga ttttaatcca tcttcaattc aatttcctgg
      901 cgccattgta aaagcatatc tttatttaat gcctgtatgc cttctttcgt tactccgctt
      961 ggaattggaa caaattcatt tagtctatca aaaacaataa atcggttttc actactaaca
     1021 agctggagaa gatgccacaa tgaaagtgat tcctttttag taattaaaga tagaattgtt
     1081 cctatagatg catcattaac gccgctaaaa ttttcaagca gacttattaa ctcaggtgaa
     1141 gaatctgaag gatatggtat ggcataccta cccctggaaa tcaagcatcc aaaatttttg
     1201 ggcacatacg aatcaaattc ttttatattt acaataaccc agccgctttc tacggcaagc
     1261 ttacagttac cattttcatc aacgtttaaa cgaaatgcac cgcctttata caattctgta
     1321 aactttgcta atggagttaa tacagtaaac acttcagaag catcgccttc aaactttctg
     1381 atttgtcctt tttctaaact tatttcattg ctgtttttag ttcttgaaac gattgaagag
     1441 ttatcaatta acaatcttcc catcatagga attgcgaccg ttacggatga attttcatca
     1501 gtttcaatgg tggtttcagc aataaagcta tcgttcttat tcatcggact attatctact
     1561 ttgggctgac cagagataga aattacttcc caaccactgc ttggagatgt taaaatagta
     1621 taaacgatta ttattgcgat caacgccaaa actgtgaaac caagtttttt aaaaagttga
     1681 ggattaaact taaacttctt tttggatttt actttttctt cctcaacata cttctctttt
     1741 tctataggaa tctcttcctc gtctttttct tgtttttctt ccgtacttaa ggcctgtgct
     1801 attttttcct gttctttctg ttcgcgaagt tttacatcca ttatgtagga aaatattcca
     1861 ttacgcactt ccgttgaagg ctgaagttcc ggcacaactt tagcaatttt ttccttcaac
     1921 actgcctcat cggaaagtgt tggttcgctg cttgcgagtt tagaaatagc catccctaaa
     1981 tgaaccgtta cctgatcttt tttaattc
//
LOCUS       DASWBY010000737         5265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_399110, whole genome shotgun sequence.
ACCESSION   DASWBY010000737 DASWBY010000000
VERSION     DASWBY010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399110"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..860
                     /locus_tag="VF870_13320"
     CDS             105..860
                     /locus_tag="VF870_13320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX9253219.1"
                     /translation="MLNLNSKKILITGGSRGIGAACVKYFCMASASVAFTYNSNPTAA
                     ARLEKRFSKISKCKAYKVDITNEDEIKKTVQSVVKDFGRIDVLVNNAGIWKEAKISNM
                     KLSAWEETIKTNLTSTFIFTQQVIHYMKLKRYGKIINISSTAGQRGEAHYSHYAASKG
                     GMISFTKSLASELGPFNINVNSVAPGWVLTDMSKSVLSNKKSLDEELKKIPLGRIATA
                     DDIAGPVLFLASDISRHINGEILNVNGGSVLVG"
     gene            868..1323
                     /locus_tag="VF870_13325"
     CDS             868..1323
                     /locus_tag="VF870_13325"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX9253220.1"
                     /translation="MEFLASIHPKIVHFPIAFLMLYPVMELIYLITGKDFFSKTAMLF
                     LFIGVLGSVIAVLSGNQAYQLVNNWTDEGKKIFESHQTFANLTVWYFSVLLVVRYFLF
                     IKKKLNRTFISIIFVLSLLGGYFVYQAGNYGGKLADQVIINSTSSFEDE"
     gene            1364..1963
                     /locus_tag="VF870_13330"
     CDS             1364..1963
                     /locus_tag="VF870_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253221.1"
                     /translation="MIKAIKYLLITIIIFSSVGFAENPPSGKATGFFVAFGVGPRMPV
                     GDFANTTDIGYGVNVEFSYTDNEFLPVFLFANIGYEQYPGSQNYFQETDYSNFHTNAL
                     PVNLGARYYFPPLLENIVLLMPIVQASASYCYYQTLHEFDQNSGKNNFLNDESKFGFS
                     AGAGVSMFMMELLASYNYMPSNQFISVDLKVRIPLYINF"
     gene            1973..2755
                     /locus_tag="VF870_13335"
     CDS             1973..2755
                     /locus_tag="VF870_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9253222.1"
                     /translation="MNYKNLLLDINEHIALVSLNRPDKLNALNHETLSELQQVFEKLK
                     VDENIFVVILTGSGEKAFVAGADISEINKLNMLEGKEFAEFGQSVFSMIEKFDKPVIA
                     AVNGFALGGGCELALSCHIRLASENAKFGQPEVNLGIIPGYGGTQRLTRLINSGRAAE
                     IILTADMIDASEALRIGLVNKVYPQGELQSKAFEMAAKIAGKGQQAVRLALKAIKVVD
                     EVSSHEGQNIEAALFALCCGTEDFKEGTQAFLEKRKPSFTNK"
     gene            2871..3890
                     /locus_tag="VF870_13340"
     CDS             2871..3890
                     /locus_tag="VF870_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253223.1"
                     /translation="MIKSSLLKNIALILFGIVVLLLITDLIITKYINKDEQPKNRKAL
                     SGIEIDRSFHSALKNYGFSDSWITKTKLKNISGDSLYASYSVKVPKDVPIQLLILELK
                     NIFWEDDVNLEAEEKVSGKKSLVQLSSENDLMLAAEFYYDENAVRQFGTVSFLVNDLP
                     LQDEENLNSFLNVPELFYIVLSPREESKKRLSLLSKAGKRYALLLDDNITELNFKLSS
                     SYSDDKIKKSIKEIVGTFYNAAFFIIDDKSDLFESKKYPLIESELLKRGITLVHSSKF
                     ETLKSSKINAADKFQDFMLTVQKNDEKVLMVSANDFLTISALIPSYRKIGYKFIYPGD
                     IIIKR"
     gene            complement(4063..>5265)
                     /locus_tag="VF870_13345"
     CDS             complement(4063..>5265)
                     /locus_tag="VF870_13345"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9253224.1"
                     /translation="SKYDPENKKGLSYFTSMLLDEGADGMDALQLSDEFDMLGSSFNI
                     SADNDLINLSLQTLSENFERSLELFSKVLLKPSFNDEDFTREKKKLITRIMQSKDEPE
                     YLADQIFDRVVLGKSNNYSFPVSGYEETVESITVDEIKTHYKNFFSPSNSSLIVVGDV
                     MQDELRNLLNKYLANWKSTQNDFSFSVESIEPKKKIYIYHKDGSVQTEIRVGHITPKR
                     NEEDFFQRYILNTILGGQFTSRINLNLRERNGYTYGATSRFQYFKDSAFFEVSTSVGI
                     ENTVNALKEILFELDNIQNGVTEKELEFAKSSITKKFPLNFETYRQIASGVAGKILYN
                     LPDNYFDTYIHNVNSVLKTEVDKAAKEFIMNDKLSIVLVGDKNLLLPKLDELKIEVAE
                     VDLFGEVI"
BASE COUNT         1785 a          802 c          883 g         1795 t
ORIGIN      
        1 cctgaaaatt ttattcaaga catatttaac tcagaagacc gggaagaagc atttgaagct
       61 gttccggcta aaggactatt tttatataaa gtggagtact aaaaatgtta aatctaaact
      121 caaagaaaat tttaattaca ggcggatcaa gaggtattgg tgcggcgtgt gtaaaatatt
      181 tttgtatggc cagtgcatcc gttgctttta cttataattc aaatcctact gcagctgcaa
      241 gacttgaaaa acgattttca aaaatatcaa aatgcaaagc atacaaagtt gatattacaa
      301 atgaagatga aattaaaaag actgtacaat cagtggtaaa agattttggc aggattgatg
      361 tcttagtaaa caatgccgga atctggaaag aagcaaaaat tagtaatatg aaactttccg
      421 cctgggaaga aaccatcaaa acaaacctta cttcaacatt tatttttact cagcaagtaa
      481 ttcattacat gaaattaaaa agatatggta aaattattaa catctcctca acagcagggc
      541 aaagggggga agcacattac tcacactacg cagcttctaa agggggaatg atatcattta
      601 caaaatctct tgcatcagaa cttggacctt ttaatataaa tgtaaacagc gttgcaccgg
      661 gctgggtttt aaccgatatg tctaaaagtg ttttatcaaa taaaaaaagc ttagatgaag
      721 aattgaagaa aattcctcta ggcagaattg caactgcaga tgatattgcc gggcctgttt
      781 tatttcttgc ttccgatatc tcaagacaca taaatggtga gattttaaat gtaaatggcg
      841 gttcagtttt agtaggataa taaatagatg gaatttttag caagcataca tccaaaaatt
      901 gttcattttc ctatcgcatt tttaatgctc tatcctgtaa tggaattaat ctatttaatt
      961 accggaaaag attttttttc caaaactgca atgttattcc tttttatcgg agttttgggt
     1021 tcagttattg ctgtactttc tggtaatcag gcatatcaac ttgtaaacaa ttggacggat
     1081 gaaggaaaga aaatttttga aagccatcag acttttgcca atttaactgt atggtatttt
     1141 tctgttttgc ttgttgtaag atatttttta tttattaaga aaaaacttaa tcgaacattt
     1201 attagtatta tctttgtatt gtctttactt ggtggttatt ttgtttacca ggccggtaat
     1261 tacggaggta aattagcaga ccaggttata ataaattcta cttcatcgtt tgaagatgaa
     1321 tagtaaattt ggatatcaga tttttaacta gttttatttt acaatgatta aagcaataaa
     1381 atatttatta attacgatta ttattttttc ttcagttggg tttgcagaaa atccgccttc
     1441 tggtaaagct acaggatttt ttgtagcatt cggagtggga ccaagaatgc cggttggaga
     1501 ttttgctaac acgactgata ttggttatgg agtgaatgtg gaattttcct atacggataa
     1561 cgaatttctt ccagtgtttc tttttgcaaa tataggatat gaacaatatc ccggttcaca
     1621 gaattatttt caggaaactg attattcaaa ttttcataca aatgctttac cagttaattt
     1681 aggtgcaaga tattattttc caccattatt agaaaacatt gttttactaa tgcccatcgt
     1741 tcaagcatct gcttcatatt gttattatca aactttacat gagttcgatc aaaattcagg
     1801 taaaaataat tttcttaatg atgaaagtaa gttcggattc agtgccggtg ccggtgtttc
     1861 aatgtttatg atggaattgt tagcttctta taattacatg ccttcaaatc aatttatttc
     1921 tgttgatcta aaagtaagaa tcccgctata tataaatttt taagagagag ttatgaatta
     1981 caaaaattta cttctcgata ttaatgagca cattgcattg gtatctttaa atcgccctga
     2041 taaattaaat gcacttaatc atgaaacatt atcagaatta cagcaagttt ttgaaaaatt
     2101 aaaagttgat gaaaatattt ttgttgttat actaaccgga agcggtgaaa aagcatttgt
     2161 tgcaggcgca gatatttccg agataaataa attgaacatg ctggaaggga aagagtttgc
     2221 tgaatttgga caatcggttt tttctatgat tgaaaaattt gataaaccgg taattgctgc
     2281 agtaaatgga tttgctcttg gaggtggttg tgaacttgca ctttcatgcc atattagatt
     2341 agcaagtgaa aatgcaaagt ttggccagcc ggaagttaac cttgggataa ttccgggata
     2401 cggtggaact cagagattaa cgagattaat taattccggt agagcagcag aaatcatttt
     2461 aactgccgat atgattgatg cctctgaagc tttaaggatt ggattggtta ataaggttta
     2521 tccgcaaggc gaattgcaaa gcaaggcttt tgaaatggct gctaagattg caggtaaagg
     2581 acagcaggct gttcgcttag cattaaaagc aataaaagta gttgatgaag tttcatcgca
     2641 cgaaggacaa aatatagaag cagctttatt cgcattgtgc tgcggtacag aagattttaa
     2701 agaaggtaca caagcatttt tagaaaaaag aaagccatca tttactaata aataaaatag
     2761 gattttttta tccctaaatt ttcattaaaa cattcagtct gtaatcatac aaataaacta
     2821 gtgcttgtac ttgaacttaa acttgaactt aatcataatc ttaaactcaa atgattaaat
     2881 cttctcttct aaaaaatatt gccctgattt tatttggcat tgttgtatta ctgctcatta
     2941 cggacctaat tattaccaag tatataaata aagatgaaca acctaaaaac aggaaagcac
     3001 tcagtggaat tgaaattgat cgatcatttc actcggcgct taaaaattat ggattttcgg
     3061 atagttggat tactaaaact aaattaaaaa atatttccgg cgattcactc tatgcatcat
     3121 attctgttaa agttcctaag gatgttccta ttcagttatt gattcttgaa ttaaaaaata
     3181 ttttttggga agatgatgtt aatctggaag cagaagaaaa ggtttccggc aaaaaatcat
     3241 tagtgcaatt atcatccgaa aatgatttga tgcttgctgc agaattttat tatgatgaaa
     3301 atgcagttcg ccaattcggg actgtttcat ttcttgtaaa tgatttaccg ttacaggatg
     3361 aagaaaacct taattcattt ttaaacgtac ctgaactatt ttacatagtt ctttctccca
     3421 gggaagaatc aaaaaaaaga ttatcgctat tatccaaagc gggtaaaaga tatgcattat
     3481 tgctggacga taatatcact gaattaaatt ttaaactttc cagcagctac tcagatgaca
     3541 agatcaaaaa atctattaaa gaaattgttg ggacgtttta caatgcggct ttttttatta
     3601 ttgatgataa atcagattta tttgaatcaa aaaagtatcc tttaatagaa tctgagttgt
     3661 tgaagagagg aattacattg gttcactcct ccaaatttga aactttaaaa tcatcaaaaa
     3721 ttaatgcagc agataagttt caggatttta tgttaacagt tcagaagaat gatgaaaaag
     3781 ttttaatggt aagtgcaaat gactttttaa ctatctctgc cttaatccct tcttacagga
     3841 aaattggtta taagtttatt tatcccggcg atataataat taaaagataa cgattatcgc
     3901 actgagcttt tcaaatcgtc atgctgaact tgtttcagca tcttattcgt caataaattt
     3961 agaacctgat acaagttcag ggtgacaatc tgatgttata aaagatataa ttacagcgtc
     4021 aatcaaataa aataattttt ggatcttcac taagagaatt tattaaataa cttcaccaaa
     4081 caaatcaact tctgccacct caatttttaa ctcatcaagt tttggcaata ataaattttt
     4141 atcgccaaca agaactatgc ttagcttatc attcattata aattcttttg cggccttatc
     4201 aacttctgtt tttaaaactg aattcacatt gtgaatgtat gtgtcgaaat aattatcggg
     4261 taaattgtac agaatttttc cggcgactcc tgaagcaatc tgtcgataag tttcaaagtt
     4321 aagcggaaac ttttttgtga tcgatgattt tgcaaattcc aattctttct ctgtaactcc
     4381 gttttgaata ttatcgagtt caaaaagaat ttcttttaaa gcattaacgg tgttttcaat
     4441 tcctaccgaa gttgaaactt caaaaaatgc agagtctttg aaatattgga atcttgaagt
     4501 agccccgtaa gtatatccat tacgttcgcg aagattaaga ttgattctgc tggtaaattg
     4561 tccgcctaaa attgtattaa gaatatatct ttgaaaaaaa tcttcttcat ttcttttagg
     4621 tgtaatgtgt ccaacccgta tttcagtttg aaccgatccg tctttgtgat aaatataaat
     4681 tttcttcttt ggttcaattg attcaactga aaatgaaaaa tcattttgag tacttttcca
     4741 atttgctaaa tatttattta ataagttcct tagttcatct tgcattacat ctccaaccac
     4801 aattaaagat gaatttgaag gggagaagaa gtttttataa tgagttttta tttcatcaac
     4861 agtaattgat tcaactgttt cttcataacc tgaaacgggg aatgagtagt tattagattt
     4921 acctaagaca accctatcaa aaatctgatc tgccaaatat tcgggttcat ccttactttg
     4981 cataattctt gtaatgagct tctttttctc tctggtaaaa tcttcatcat taaaagaagg
     5041 cttaagcaaa actttactaa acaattccaa tgatctttca aaattctccg aaagggtttg
     5101 caaacttaaa tttatcagat cattatccgc cgagatatta aaacttgaac cgagcatatc
     5161 aaactcatca ctcaactgca gagcatccat tccatcggcg ccttcatcta aaagcattga
     5221 agtaaaataa gacaaacctt ttttattttc tggatcatat ttgct
//
LOCUS       DASWBY010000738         5941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_328044, whole genome shotgun sequence.
ACCESSION   DASWBY010000738 DASWBY010000000
VERSION     DASWBY010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328044"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VF870_13350"
     CDS             <1..321
                     /locus_tag="VF870_13350"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX9253225.1"
                     /translation="MKINDTAEGDLPRFSKVKHPRTGIGFSEDSTLVYFITVDGRQES
                     SSGMTLMEFADLMIEEGIYQGLNLDGGGSTTMVVNSKVVNSPSDITGERKVGNCIVVI
                     KKQD"
     gene            complement(528..1454)
                     /locus_tag="VF870_13355"
     CDS             complement(528..1454)
                     /locus_tag="VF870_13355"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9253226.1"
                     /translation="MKLYLKSILSLILIGVMCNSNFSQVIDLNEKWSKAADGLNFPEG
                     PAYDGKASIYLSNCYGGWITKYSNGIKDTFVSKVNDTLLIEKTNGLAYGKDGYLYVCE
                     YGKGKILKIGNDARIEVYADGYKGEKFNRPNDITIDKNGNLFFTDPKSYDKNILDGRI
                     FLFDAKTKRVVLVQDSLAFPNGINISPIDGRLYVCESAKQKIVRFKITPENKLVDKED
                     FAELPGGDPDGIEFDINGNLYVAHFGGKAVYVFSPEGEQIQKIETPGVKPTNLEFGDE
                     DYKTLYLTEVETNALYKIRTRIAGKNILNKTN"
     gene            1555..2574
                     /locus_tag="VF870_13360"
     CDS             1555..2574
                     /locus_tag="VF870_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9253227.1"
                     /translation="MKHFKFNVFITLIFSASILFSQSVQENASIGDLKLVNGNKIENC
                     IMGYRTFGTINNDSSNVILYCSWFEGTSEAIGTLIQKYKFIDTTKYYIIAVDALGNGI
                     SSSISNYELDDSVFYNITITDMVNANYSLLTQQLKIKKLYAAIGGSMGSMQVLQLAVS
                     YPEYADRIIAYVATPRMSSYDLLWMNTQLNMIESSKKCGMSEREIKKILDMNTALFAR
                     TPDYVVENTDLSEFPEYLKSFDKEPSKSFILDNYLTQLRAMMKHDISTNFNGSMDEAS
                     KNIRARLFIIVSKTDMMVNPTEALNLARLTNAKTLILNNNCGHLAVNCDIDLCREEIA
                     KFLME"
     gene            complement(2648..3112)
                     /locus_tag="VF870_13365"
     CDS             complement(2648..3112)
                     /locus_tag="VF870_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9253228.1"
                     /translation="MQMESIESFLKLKNIAVVGVSSKGKGFGVSVYKHLKDNGFTVFG
                     VNKYGGFSDSVKLYNSISMIADKIDGVITVVPPSETEVIVQEAKELGINNIWMQQGSE
                     SKNAIAFCNENKMNVVHNECIMMFVEPVKSIHSFHRWINKLVGKYPSMEKPR"
     gene            3284..4765
                     /gene="purF"
                     /locus_tag="VF870_13370"
     CDS             3284..4765
                     /gene="purF"
                     /locus_tag="VF870_13370"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX9253229.1"
                     /translation="MNYEEDKPISYCGIFGIYGSKTASAQAYYGLHALQHRGQEASGI
                     VTRSFNGDNRAHFNIVKGEGLVSEVFADHNLLTNLLVGNSAIGHNRYSTTGAAKSRKN
                     IQPFMVNYRMGNLAIGHNGNLTNAKVLRKELVNEGAIFQTTSDTEVILHLIARSKLDN
                     QIDQIMEALKRIEGAYCLVILTDDKLIAARDPLGFRPLSLGKLNDSFLVASETCAFDI
                     QRAEFLREVEPGELIVIDDESIKDGEIKSYRINTNPEQKKHCVFEYIYFSRPDSFIFG
                     HNVDKMRRRLGKELARSHPVTDPDGERVYVISVPDSSNTTALGYARELEKQGIDARLE
                     IGLIRSHYIGRTFIQPGQTNRETGVRIKFNIVKGVLEGRTIVVVDDSIVRGTTSKQLV
                     HLLREANPKSIHLRISSPPIMHPCFYGMDFPSSQELIAFQKNGNVEEIKKYLEVDSLE
                     YLTIDEMLEAVSEAGKENFCTACFSGKYPTNVDTNFKKEMYEV"
     gene            4755..>5941
                     /locus_tag="VF870_13375"
     CDS             4755..>5941
                     /locus_tag="VF870_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9253230.1"
                     /translation="MKFNRLLILATLILLTIQSFAQTTYFIKYKSNVPMDVVESNISN
                     RIISDAIGDRPVSLPNYNIDYLAKGLGREDEVIGRIVKVQFDENVDETNFSSLLSTDP
                     NIEYIQRSSTYQLDYIPNDSLLSQQWALQKIKAFDAWDITQGADTVLLGIIDKGIDYN
                     HSDLKNKIFINPGETGTDQFGNDKTSNGIDDDGNGFIDDYRGWDFTDRVGFPFDSTGG
                     DYLGWDNNPFDDQGHGTYITGIAAAQTNNVSGIAGTAPNIKVLNLRAFDPGGYGEEDD
                     VAAAILYAVVMGAKVINMSFGDDAFSYVLRDVIRYAYSQNVVLIASAGNSGSSAPHYP
                     SGYSEVICVGNSTDQDYVAGSSNFGSTVDLVAPGSLIMTTSKNSGYSVISGTSASAPF
                     VSAA"
BASE COUNT         1984 a          976 c         1036 g         1945 t
ORIGIN      
        1 atgaaaataa atgatactgc cgaaggtgat ctccccagat tttcaaaagt aaaacatcca
       61 agaaccggaa ttggtttttc tgaagacagt actttagttt attttatcac tgttgatgga
      121 agacaggaat caagcagcgg aatgacatta atggaatttg cggatttaat gattgaagaa
      181 ggaatttatc agggattaaa tcttgatggc ggtggttcta ctacaatggt tgtaaatagt
      241 aaagttgtca atagtccttc agatattacc ggcgaaagga aagttggcaa ttgcatcgta
      301 gtaattaaaa agcaggattg agagttgtaa acttggttaa gaaagttgtc attaacttgt
      361 ctatcaggtg ttttatttgt cattcccgtg aaaacgggaa tacaaaatcc gtaatattaa
      421 atggattccc actttcgtag gaatgacaat tttaattttt gacttttaaa ttgacacatt
      481 ccgtatttga ttttgaataa gtctaataac atttaaacct gttttaatta attagtttta
      541 ttcaaaatat tctttccggc aattcgagtt ctgattttat acaaagcatt ggtctctact
      601 tcagtcaaat agagagtttt ataatcctca tcgccaaact caagatttgt tggtttaaca
      661 ccgggagttt caatcttttg tatttgctct ccttcaggtg aaaatacata aactgcttta
      721 cctccaaagt gggctacata taaatttcca ttaatatcaa attcaattcc gtcaggatca
      781 ccacccggta attctgcaaa atcttcctta tcgaccagtt tattttccgg agtaattttg
      841 aatcgaacta ttttttgttt tgcagattca cacacataaa gtcttccgtc aattggtgaa
      901 atattaattc catttgggaa ggctaaactg tcttgaacca gaaccactct tttagttttt
      961 gcatcaaaca aaaatattct gccgtccaaa atatttttat cataactttt tggatcagta
     1021 aaaaataaat ttccattttt atcaattgtt atatcgtttg ggcgattaaa cttttctcct
     1081 ttgtaaccgt cagcataaac ctcaatccgc gcatcatttc ctatttttaa tatctttcct
     1141 ttaccatatt cacaaacata cagataccca tctttaccat aagctaagcc attcgttttt
     1201 tcgataagta aagtatcatt cactttagat acaaaagtat cttttattcc attagagtat
     1261 ttagtaatcc atccgccgta acaattggaa agatatatag atgctttgcc atcataagct
     1321 ggaccttcag gaaaatttag tccatcagca gcctttgacc atttttcatt caaatctatt
     1381 acctgagaaa aattagaatt gcacataact cctattagaa tcaaggataa gatggattta
     1441 agatacagtt tcattttaat tcctggaaat attttaataa ttcttcttta tactttaaca
     1501 aatttataaa ttttaagtga atatatttta attttataaa acattagtac atttatgaaa
     1561 cacttcaaat ttaacgtttt catcacactt atattttcag cttcgattct attttcccaa
     1621 tcagttcagg aaaatgcaag tataggcgat cttaaacttg taaatggcaa taaaattgaa
     1681 aattgcatta tgggctacag aacttttgga acgataaaca atgattcctc aaatgtaatt
     1741 ctttattgta gttggtttga aggaacttca gaagcaatcg gaacactgat tcaaaaatat
     1801 aaatttatag atacaacaaa atattatata atcgctgttg atgctttggg aaatgggata
     1861 tcctcttcca tctcaaacta cgagcttgat gattcagtgt tctataatat tacaataact
     1921 gatatggtca atgctaatta ttctttattg acacagcaat taaaaataaa aaaactttat
     1981 gctgctattg gcggttcgat gggtagtatg caggttctgc aattagctgt tagctatccc
     2041 gaatatgctg accgaattat tgcttacgtt gcaactccaa gaatgtccag ctacgattta
     2101 ctgtggatga acactcagct taatatgatc gaatcatcaa aaaaatgcgg aatgtctgaa
     2161 agagaaataa aaaaaatctt agatatgaat actgcactat ttgcaagaac acctgattat
     2221 gttgttgaaa atacggattt atctgagttt cctgaatacc tgaaatcatt tgataaagaa
     2281 ccaagtaaaa gttttattct tgataactat ctgactcaat tacgtgctat gatgaaacac
     2341 gatatatcta ctaattttaa tggctcaatg gatgaagcat caaaaaatat tcgtgctaga
     2401 ctatttataa ttgtaagtaa aaccgatatg atggtcaatc caactgaggc tttgaatctg
     2461 gcaagattga caaacgcaaa aaccctgatc cttaataaca attgtggaca tctcgctgta
     2521 aactgcgata tagatctttg tagagaagag atagcaaagt ttttaatgga ataaagaaat
     2581 tggggaataa tggaattagt acgaattgaa ttccatcatt ccgcaattcg aatattctaa
     2641 taaatgttta ccgcggtttt tccatacttg gatacttacc aaccaattta tttatccagc
     2701 gatgaaaact gtggattgat ttaacgggtt caacgaacat cataatgcat tcattgtgta
     2761 caacattcat tttattttcg ttacaaaaag ctattgcatt tttggactca gaaccctgct
     2821 gcatccaaat attatttatt cccagttctt tagcttcctg tacaattact tcagtttcag
     2881 aaggcggtac aacggtaatg actccatcaa ttttatcagc aatcatagaa attgagttat
     2941 ataattttac actgtctgaa aatccaccgt acttattaac accaaaaact gtgaatccat
     3001 tgtccttaag atgcttataa acagaaaccc caaaaccttt accttttgat gaaacaccta
     3061 cgactgcaat gttttttagt ttaagaaatg attcaattga ttccatttgc atatattttc
     3121 tcctctgaaa tttacaatcc aaaattacga ttaaatattc agaattttta atgaattatt
     3181 tcaacaaaaa ttcagcatat tggcacatca aaatgatttc ataaaatatc ttcatttact
     3241 taactttgtt tgcaaattat ttacaactta atctcaagaa aaaatgaatt acgaagaaga
     3301 taaaccgatt tcttactgtg gtatttttgg aatttacggt tctaagacag cttctgcaca
     3361 agcatattat ggattgcacg ctttgcagca tcgagggcag gaggcaagtg gaattgtgac
     3421 ccgctctttt aacggcgata atcgtgcaca ctttaatatt gtaaaaggtg aaggattagt
     3481 gtctgaagtt tttgcagatc acaatcttct aacaaattta ttagttggta attcggctat
     3541 aggacataac aggtattcaa ctacaggtgc agcaaaatca cgaaaaaata ttcagccttt
     3601 tatggttaat tacagaatgg gcaatcttgc aattgggcat aacggaaatc ttaccaacgc
     3661 aaaagtgcta agaaaagaat tagtaaatga gggagcgata tttcaaacta cgagtgatac
     3721 tgaagttatt cttcacctga ttgcccgcag taaacttgat aatcaaatag atcaaataat
     3781 ggaagcacta aaaaggattg aaggtgctta ttgtttagta attctaaccg atgataaatt
     3841 aattgctgcc cgtgatccgc ttggctttcg tcctttatca ttaggcaaat taaatgactc
     3901 ttttcttgtt gcttcggaaa cctgtgcatt tgatatacaa cgagcagaat ttttaagaga
     3961 agttgagccc ggagaactaa ttgtaattga tgatgaatca attaaggatg gtgaaattaa
     4021 atcatatcga attaacacaa atcctgaaca aaaaaaacat tgcgtttttg agtacatata
     4081 tttctctcgt cctgatagtt ttatttttgg acataacgtt gataagatgc gacgaagatt
     4141 gggaaaagaa cttgcgcgtt cacaccccgt tactgatcct gatggtgaga gagtatacgt
     4201 aatcagcgtt ccggacagct caaacacaac agcactcggt tatgcaaggg aattagagaa
     4261 gcagggaatt gatgcacgtt tggaaattgg tttaatcaga agccattata tcggaagaac
     4321 atttattcaa cccggacaaa caaatcgtga aaccggagtt agaattaaat ttaatattgt
     4381 aaaaggagtt ttggaaggaa gaacaattgt tgttgttgat gactcaatcg ttcgaggaac
     4441 aacatctaaa caattagtac atttgttaag ggaagcaaat ccaaaatcaa ttcatttaag
     4501 aatttcttct ccacctatta tgcacccgtg tttttacgga atggatttcc ctagcagcca
     4561 ggaattaatt gcatttcaaa aaaatggaaa tgttgaagag ataaaaaagt atcttgaagt
     4621 agatagcctg gaatacctaa ctatcgatga gatgttggaa gcagtttctg aagcgggcaa
     4681 agaaaatttt tgcacagctt gtttctccgg aaaatatcca acaaatgtag ataccaactt
     4741 caagaaggag atgtatgaag tttaaccggt tgttgattct tgccacatta attttattaa
     4801 ccatccaatc atttgcacaa acaacatatt ttataaagta taaaagtaat gtgcctatgg
     4861 atgttgttga gtctaatatt tcaaatcgga tcatttcaga tgcaattggt gatagacctg
     4921 tttcacttcc aaattataac atagactatc ttgcaaaagg tttgggcagg gaggatgaag
     4981 taattggccg aattgtaaaa gtacaattcg atgagaatgt tgatgaaaca aactttagtt
     5041 ctttattatc aaccgacccg aatatagaat acatacaaag atcttcaacc taccagttag
     5101 attatatacc gaatgacagc ttgctttccc aacaatgggc attgcaaaaa attaaagcct
     5161 ttgatgcatg ggatataacc cagggtgctg atacagtttt gcttggaata attgacaaag
     5221 gtattgatta caaccactct gatttaaaaa ataaaatttt tattaatcct ggtgaaacag
     5281 gcacagatca gtttggaaat gataaaacaa gtaatggtat tgatgatgat ggaaacggtt
     5341 ttattgatga ttatagaggc tgggatttta cggatcgtgt tggatttcct tttgattcaa
     5401 caggtggtga ttatttaggt tgggataaca atccgttcga tgaccaggga catggaactt
     5461 atataactgg tattgccgct gctcaaacga acaatgtaag tggaattgcc ggtactgcac
     5521 caaatataaa agttttaaat cttcgcgctt ttgatcccgg tggttacggc gaagaagatg
     5581 atgttgctgc agccatttta tatgcagtgg taatgggagc aaaagtaata aatatgagtt
     5641 ttggtgatga tgctttctct tatgttctaa gagatgttat tcgatatgct tattcacaga
     5701 atgttgtatt gattgccagc gcaggtaact ctggctcaag tgctccgcat tatccttcag
     5761 gttattctga agttatttgt gtcggtaatt caacagatca ggattatgta gcaggaagtt
     5821 caaacttcgg ttcaactgtt gatttagttg ctccaggttc tcttataatg acaacatcaa
     5881 aaaattcagg atattctgtt attagtggaa catctgcatc ggcacctttt gtttctgcag
     5941 c
//
LOCUS       DASWBY010000739         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_367885, whole genome shotgun sequence.
ACCESSION   DASWBY010000739 DASWBY010000000
VERSION     DASWBY010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367885"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VF870_13380"
     CDS             <1..744
                     /locus_tag="VF870_13380"
                     /inference="COORDINATES: protein motif:HMM:NF020416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9253231.1"
                     /translation="NSEDFARDVLSAVEFLKARKEIDKTKIGLIGHSEGGIIAPLAAV
                     QSSDVAFIVMMAGLGIPGDSILFLQGTLIQKAEGTSEEEIQKSIKLQREIFSIIKNNN
                     DDEKIDKELKEKFYAEYAAMTEEEKSKLGDPEVFVNMQIKTIASPWFKYFLRFDPAPV
                     LEKVKCPVLAINGEKDLQVPPKENLSAIESALKKGGNQNFEVKMLPGLNHLFQTTTTG
                     KISEYGKIEETISPLALDTMLEWIKKVTK"
     gene            894..1598
                     /locus_tag="VF870_13385"
     CDS             894..1598
                     /locus_tag="VF870_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX9253232.1"
                     /translation="MSLAEIRKEILKQKNPVQAINLQRFFKTGKGEYGEGDIFYGIKV
                     PEQRIIAKQFSDLSLDDLKILITSKVHEERLIAVFILVEQFKKGDDKKQKIIYDFYLK
                     NRKGINNWDLVDLSAPKIAGAYLIDKEKDQLYKFANSKDLWEKRISIISTQAFIREHF
                     FEDTLNISEILLNDKHDLIHKAVGWMLREIGNRDLEIEEEFLKKYYKTMPRTMLRYAI
                     EKFPERKRKSYLEGKI"
     gene            1595..>1905
                     /locus_tag="VF870_13390"
     CDS             1595..>1905
                     /locus_tag="VF870_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9253233.1"
                     /translation="MSRKKISSGAKWEDIVGYSRAVRIGSIVEVSGTAPVDGDKVIGI
                     GDAYEQTRFCLQKIEKALIEAGAKLKDVIRTRMFVTDITLWEEYGKAHGEFFKDIKPV
                     TS"
BASE COUNT          736 a          251 c          373 g          545 t
ORIGIN      
        1 aacagtgaag attttgcaag agatgtttta tctgcagtag aatttttaaa agcacgaaaa
       61 gaaatagata aaactaaaat tggtttaatt gggcacagcg aaggcggaat tattgctcct
      121 ttagccgctg tgcaaagcag tgatgttgct tttattgtga tgatggcagg acttggtatt
      181 ccgggcgatt caattctttt tctgcagggt acattgattc aaaaagctga aggaacaagt
      241 gaagaagaaa ttcaaaaatc tataaaattg caaagagaaa tattttcaat cattaaaaac
      301 aataatgatg acgagaaaat tgataaagaa ttaaaagaaa aattttatgc tgagtatgct
      361 gcaatgactg aagaagaaaa aagtaagctt ggcgatcctg aagtttttgt aaatatgcag
      421 attaagacga ttgcaagtcc ctggtttaaa tattttttac gatttgatcc tgcaccggtt
      481 ttagaaaagg tgaaatgtcc tgttcttgca ataaatggtg aaaaggattt acaagttccg
      541 ccaaaagaaa atttatctgc aattgaatct gcattaaaaa aaggcggcaa tcaaaacttt
      601 gaagtaaaga tgcttcctgg tttgaaccat ttattccaaa caacaacaac gggcaaaatt
      661 tctgagtatg gaaagattga agaaacaatt tctccattag ctttagatac aatgctggaa
      721 tggattaaga aagtaacaaa ataaaataat tagtcatttc gaacgaagag agaaatctga
      781 aatctattat aaagcagatt tctcagtcga agactccttc gaaatgactt tgtatccatc
      841 taagtctata tggaatttca tttcattagt taaagaatag attgaatatc aaaatgagtt
      901 tagcggaaat aagaaaagaa atactaaaac aaaaaaatcc tgtacaggca attaacctgc
      961 aaagattttt taaaacgggt aaaggtgagt acggtgaagg cgacattttt tatggaataa
     1021 aagttcctga gcaaagaatt atagcaaaac agtttagtga tttgtcatta gatgatttaa
     1081 aaatattaat aacatcaaaa gttcacgaag aaagattaat cgcagtattt attctggttg
     1141 aacaattcaa aaagggagat gataaaaagc aaaaaataat ttatgatttt tatctgaaga
     1201 acagaaaagg aattaataac tgggacttgg ttgatctttc tgctcctaaa atagccgggg
     1261 catatctaat cgataaagaa aaagatcagc tttataaatt tgcgaattca aaagatttat
     1321 gggaaaaaag aatctcaatt atttctactc aagcttttat acgcgagcat ttttttgagg
     1381 acacattaaa catttctgaa atattgctta atgataaaca cgatctcatt cacaaagctg
     1441 ttggttggat gctcagagaa atcggtaatc gtgatctgga aatagaagaa gagtttttaa
     1501 agaaatatta taaaacaatg ccgcgaacga tgcttcgtta cgcaattgaa aagtttcctg
     1561 agagaaaacg taaatcatat ttggaaggaa aaatatgagt cgaaaaaaaa tttcatccgg
     1621 tgcaaaatgg gaagatattg tcggttactc acgtgcagta cgaattggca gcatagttga
     1681 agtttcaggt acagcacctg tagatggtga taaagtgatt gggattggcg atgcttatga
     1741 acaaacaaga ttctgcttac agaaaattga aaaagcatta attgaagcag gtgcaaaatt
     1801 aaaagatgtt attagaacca gaatgtttgt aaccgatatt acgctctggg aagaatatgg
     1861 gaaagcccac ggagaattct ttaaagacat aaaacctgta acttc
//
LOCUS       DASWBY010000740         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_555985, whole genome shotgun sequence.
ACCESSION   DASWBY010000740 DASWBY010000000
VERSION     DASWBY010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555985"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VF870_13395"
     CDS             <1..367
                     /locus_tag="VF870_13395"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX9253234.1"
                     /translation="WICDSAAFFGGTALGKHKLFPRVSPKKSWEGAIFGFVFAILAMI
                     LAKNIVLDFLSLKDAIIIGFIIGTFGQIGDLTESLLKRDAGVKDSSSLIPGHGGIFDR
                     FDSLLFSSPIIYLYLTQLA"
     gene            387..1676
                     /gene="rimO"
                     /locus_tag="VF870_13400"
     CDS             387..1676
                     /gene="rimO"
                     /locus_tag="VF870_13400"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX9253235.1"
                     /translation="MSGKVSVITLGCSKNTVDSERLMKQIQLNKIGLVDDPNKADTVI
                     INTCGFIEAAKEESVNTILEAVAMKNSGRIKKVIVAGCLSERYKEDLLKEIPEVDVYF
                     GTENYEGIIKELGGNLKKELLGERLLTTPSHTAYLKISEGCDHPCSFCAIPLMRGLHK
                     SKPMDELIKEAEFLAANNTKELVLIAQDTTDYGKDIYGKKNTDELLRKLSEIKEIEWI
                     RLMYAYPSQFPDELIEEIKTNPKVCKYVDIPLQHISNDVLKSMRRGVTAERTRKLLYK
                     LRKEIPDITIRTTFIVGYPNETEKDFEELCDFIREIKFDRIGTFTFSVEENTSSFILG
                     DPVSEEEKERRKNVLMEIQKEISLEKNESFVGKKLKVLVESVEGDFFVGRSYRDAPEV
                     DGEILINKSNNKIQIGKFYDVEINDYDEYDLFGDVLL"
     gene            1691..>2390
                     /locus_tag="VF870_13405"
     CDS             1691..>2390
                     /locus_tag="VF870_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253236.1"
                     /translation="MKRIALILICLSLTLNLYAQDNIGEKTKKNINDAVYYQEFLNAG
                     SDKPGLSRVDVFIQVPYKNVNFIKSNQGFTSKYSMTVSVFDSLKKQLIVEKTWNETID
                     VIDFTMTTSKDNYKLSRKSFDVAPGTYTIRTMLQDKDSKQEAVGENLFVVRDYNKEIS
                     LSDILMISKYDSLQGRNEIIPNISRNIPTSKDRVKFYFEVYSKDSTDVDKTFEYTVFN
                     SEKNVIHKEVEDRNI"
BASE COUNT          868 a          310 c          472 g          740 t
ORIGIN      
        1 ttggatttgc gattctgcag cattttttgg cggaactgcg cttggaaaac ataaactatt
       61 tccgcgtgtt agtcctaaaa aaagctggga aggagcaatt tttggatttg tatttgcaat
      121 tctggctatg atattagcaa agaatattgt tctcgatttt ttatcattaa aagatgcaat
      181 aattatcgga tttattatcg gaacatttgg tcagattggt gatctgacag aatcattatt
      241 aaaacgtgat gccggtgtta aagattcatc aagtttaatt ccggggcacg gcggaatctt
      301 tgatcgattt gattcgttat tgttttcttc accaattatt tatttgtatt taacacagtt
      361 agcatgatga tttggaataa gattttatga gtggaaaagt ttctgttata actctgggct
      421 gttcaaaaaa cactgttgat tctgaaagat tgatgaagca gatacagctt aataaaattg
      481 gattggttga tgacccaaac aaagctgata cagtaataat taatacttgc ggatttattg
      541 aagcggcaaa agaagaatct gttaatacaa ttcttgaagc cgttgcaatg aaaaacagcg
      601 gcaggattaa aaaagttatt gttgcaggat gtttatcaga gagatacaag gaagatctat
      661 taaaagaaat tccagaagta gatgtatact ttggcactga gaattacgaa ggaataatta
      721 aagaacttgg cggcaactta aaaaaagaat tgcttggtga aagattatta acaacaccat
      781 cgcatacagc gtatttaaaa atttccgaag gctgtgatca tccttgttct ttctgtgcaa
      841 ttccattaat gcgtggattg cataaatcca aaccgatgga tgagttgatt aaagaagctg
      901 aatttcttgc ggcaaacaat actaaagaat tagttcttat tgctcaggat actactgatt
      961 acggtaaaga tatttatggc aaaaaaaata ctgacgaact tttaagaaag ttaagtgaaa
     1021 taaaagaaat tgaatggata agattgatgt acgcttaccc ttcgcaattt ccggatgagt
     1081 tgatagaaga gattaaaaca aacccaaaag tttgtaagta tgtagatatt ccattacagc
     1141 atatttcaaa cgatgttttg aaatcgatgc gaaggggagt gactgcagaa cgaacaagaa
     1201 aattgcttta caaacttcga aaagaaattc cggatattac aataagaact acttttattg
     1261 ttggatatcc gaatgagact gaaaaggatt ttgaagagct ttgtgatttt attcgtgaaa
     1321 taaaattcga caggatagga acttttacat ttagtgttga agaaaataca tcaagcttta
     1381 ttcttggcga tcctgtttct gaagaagaaa aagaacgaag aaaaaatgtt ttgatggaaa
     1441 tccagaaaga gatctcgctt gaaaaaaacg aatcatttgt tgggaaaaaa cttaaagtac
     1501 tggttgaatc tgttgaaggc gatttttttg tgggcagatc atatagagat gcacccgaag
     1561 ttgatggcga aatattaata aataaaagta ataacaaaat tcaaatcggc aagttttacg
     1621 atgttgaaat taacgactac gatgagtatg atttattcgg tgatgttttg ttatgagaaa
     1681 ggaaagttaa atgaaaagaa tagcgttaat ccttatttgc ttatccttaa cattgaattt
     1741 atatgcgcag gataatattg gtgaaaaaac caagaaaaat ataaatgatg ctgtttatta
     1801 tcaggagttc ttaaatgcag gatcagataa accgggactt tcgcgtgttg atgtttttat
     1861 tcaggttcca tataagaatg tgaacttcat aaaatcaaac cagggattta catccaaata
     1921 ttcaatgaca gtttctgtat ttgacagcct taaaaagcag ttgatcgtgg aaaagacctg
     1981 gaatgaaact attgatgtaa tcgattttac aatgactact tcgaaagata actataagct
     2041 tagcagaaaa tcatttgatg ttgcccccgg gacttataca atcagaacaa tgcttcagga
     2101 taaagattcc aagcaggaag cagttggaga aaatcttttc gttgtaagag attataataa
     2161 agaaatttca ttaagcgata tcttgatgat ttcaaaatat gatagtttac aaggcaggaa
     2221 tgaaatcatt ccaaacatca gtagaaatat tccgacatct aaagatcggg taaaatttta
     2281 ttttgaagtc tattcgaaag atagtactga tgtagataaa acttttgagt atacagtttt
     2341 taattctgaa aagaatgtaa tccataaaga agttgaagat agaaatataa
//
LOCUS       DASWBY010000741         9616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_626394, whole genome shotgun sequence.
ACCESSION   DASWBY010000741 DASWBY010000000
VERSION     DASWBY010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9616
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626394"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1179
                     /locus_tag="VF870_13410"
     CDS             151..1179
                     /locus_tag="VF870_13410"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX9253237.1"
                     /translation="MMAKNKKRERKFLPVFIILAITADLAFLYFNKYNNQNLTLRDFD
                     LFYFGNIINLLFVLLLIIGLLVIIFKKSIVFNLKIFIPVFLINQFILVTLYFLKYMPL
                     PFKKLYYLGQTGDELFIGAIFFLYTLTFLILIFIVWLSIFKFKKIILFRAFINSILSM
                     IVILLLVFLFIIGKESTIKESLLKINKNTVGVVLGAAVWSDNRPSPSLAGRVDKALEL
                     YQQKIISQIFLTGSNAPGELSESEVALNYIKSKGIKTSDIFLEKATTSTNEQIQYIKK
                     KLVATANKNIIVISDSYHLVRVLEIAKFNKLNIQVSASKLSQSFDKTIYNNTREALAL
                     TMFWLYAL"
     gene            1210..1698
                     /gene="nusB"
                     /locus_tag="VF870_13415"
     CDS             1210..1698
                     /gene="nusB"
                     /locus_tag="VF870_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9253238.1"
                     /translation="MSTVFKRRVVREKVLQILYAYEMNDENLMALTKSILGDVEEEQD
                     RKYGEDLVNRVLIHKKNLDTKIEARVSNWEMGRIALIDKILLRMAICELLYFPDIPPK
                     VSINEAIEIAKEFSTAGSGKFINGILDAILSEEKTAGRLIKTGRGLVDETISKNHSKS
                     SE"
     gene            1695..2948
                     /locus_tag="VF870_13420"
     CDS             1695..2948
                     /locus_tag="VF870_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9253239.1"
                     /translation="MTDKKKIFTWTLFDFANTAFSVIIVTVIYSKYFSNYVADGRKWI
                     WGLAISLSMVIAAFLSPPLGAIADVSRNRKRFLLIFTLVSVVCTALMFFVQKGDILFG
                     FLLFVFANVGFEAGIVFYDAFLPNLTEKKNFGRVSGYGFAMGYVGALVVLLIVMVLLP
                     AQSDPNYYFYIRLSFIVAAAFFLIFSIPLFLFLREPKIEDPDKKDLVKNGIKKSVHTI
                     KEIFIRGGYPSIKRFLISFFLYNDGIITIIAFASIFAANALKMTDAEVIYFFVIVQST
                     AILGSFIFGIISDHIGPKKTITITLFIWIAVIIGTFFINTIFEFYIIGLMAGLSIGSS
                     QSSSRSLMALLIPKDREAEFFGFYDGLFGKSSAIVGPLFYGIIADLSNERVAALSIGL
                     FFIAGLIILQNVEVPEFRLLKKNKV"
     gene            complement(2974..3978)
                     /locus_tag="VF870_13425"
     CDS             complement(2974..3978)
                     /locus_tag="VF870_13425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9253240.1"
                     /translation="MNATIHKSDQLAIRKRAAIISLVVGFSMFFFKIGAYLITGSAAI
                     FSDAAESVVHVMATSMALYSIILSSRPADEFHPYGHGNVEYFSAGIEGLLIAIAAVFI
                     IYEGVVAIIAGPELKQLGIGAVVITFASVVNLLLGNFLIRTGKKTNSLTLIADGKHVL
                     TDSITSFGVITAVVLVIITDIQILDPIIAIIIALNILLTGYKLIRGSIAGLMNEVKPE
                     MLNQLSAKLVSIKKDYWIDIHGLRFWQSGDKVFIDFHLILPYYFNIKQSHEEENIIES
                     ELQKDFPQADLKIHLDYCIPQLCSFCNYAQCDVRSEEKTKSFEWNVNKLTGEPIYKWK
                     "
     gene            complement(3984..5138)
                     /locus_tag="VF870_13430"
     CDS             complement(3984..5138)
                     /locus_tag="VF870_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEX9253241.1"
                     /translation="MISKAGISSKLPDVGTSIFAVMSKMANDYNAINLSQGFPDFNCS
                     EHLVELVNHFMKSGFNQYAPMPGTVELRKQIAIKVKRDYGREYDYEKEITVTAGATQA
                     LYTAFSAIVNKGDEVIIFEPAYDSYLPAITTNGGIPISIKLNTNDFSIPWNTVEKSIT
                     KKTKCIVINSPHNPTGAILKEDDITQLKKIVEGTNIFLISDEVYEHIIFEKQKHCSFA
                     LYDELAKRSFIISSFGKTFHTTGWKMGYCCAPENLTAEFRKFHQFIVFAVNTPIQLAV
                     AELLKDENNIYSLPDFYQAKRDLFAKLLKNSKFMLKKCEGTYFQLLDYTKVSNKNELE
                     FSEYLTKEIGVAVIPLSPFYKDGSDNKLIRVCFAKKDEVLKQAAEKLDSP"
     gene            complement(5147..6718)
                     /locus_tag="VF870_13435"
     CDS             complement(5147..6718)
                     /locus_tag="VF870_13435"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9253242.1"
                     /translation="MPSKILIPFLLTLISFLFIPNRLEAQDTTRSLAFKNNRPYRNNP
                     YFYQPDLSYQILQQFKLVQIANSGDPLAQHELGLRLLTGEGIAADTAAAVYWIKKAAS
                     QKLTSALYNYGIMLINGWGTDWNPFTAYDNFLLAAEAGMSQAQYVVGILHTDNLIVQR
                     DWNKSYYWIKKSAEGGYKPAKELLAEISPKVSPAFRDSVDQNKYNLTKDTFKKLDKED
                     SKIQPSLGLVFIDFDTQSDTIKEITDAMLISDLKNLNLKVVKDSLVIKDDSSLTSFFD
                     ATKINLLRKIAESGSHEAETILGRIYETGNFVGINKLFAAEYYIRATILDSPRAAYLL
                     WKLIREKDFYTILNDAVQLGDPTAQFVWYGLQKLGYDNRLTEKDAINLLQKAAAQNHI
                     PAIIELGFNYYTGKYGLNDKQKGLEYWKLAENYNSQEASVRIAVSKVFESDQSADLKN
                     EISILETSERNGSILAQFALGYCYEKGIGVKKSLPSAVEFYRNSAQRGNQYAYEQLKR
                     LYDNLRPLDKTFNVN"
     gene            6839..9277
                     /locus_tag="VF870_13440"
     CDS             6839..9277
                     /locus_tag="VF870_13440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX9253243.1"
                     /translation="MIDKSKQIIIPLIVLIFAIIVIYFIIPEYHPFGGLRIVNNQEII
                     LKKAEDFLNKSNILYDENRLKINFDTDKNFVRWINSEYKIDEANKILYNAGSAYYWTV
                     SQDRTDDAKVIVSSNPGSNNNRNKSSFVLNILDNGKLIGFSTELADTSVKKSLSDDGA
                     KKLAEKFIKGLRADIVFVDDTLQSSNGINASTFYFKETETNNKPDRIDYNFTWQTKNK
                     TTINYYLKAQVVGDQVSNFSIQVMIPDEYKKNENDVFEIATTIIFMLLIVISVIVVGF
                     KRFRAFEVGFKQAIAFGIFVLISFIVKELLERFSSFEWTIILGLGLGGIFIAGAAIIL
                     WAVSETVFREIWNEKFLSLDLIYHRKIVHSSVGKAIINSISFGFGLTAVFFVLLFAIS
                     YYSNVSFAGENFISQSHITAKLPLLNIFFGVFNSFGILAVAFFMFLTAAIKRYINNDI
                     IFILVSGFTWSLLMQSNVNPLTAGLPINLVIGILLSLILIKYDLLSTLLSFLLFKFFI
                     KATELMFMTEASLKNEWYLLVAICLVLILIGIILVFRKDTFTDYDSITPKFVENITER
                     QRLKKELDVARHVQMSFLPKENPILKGIDIASVCIPAFEVGGDYYDFIYLGKNKLGII
                     IGDVSGKGTQAAFYMTLTKGFLKAIAKNTDSPAEVLTKMNELFYENVERGRFISMIYA
                     VVDLENKLIRIARAGHNPVIYHDSEGNINLISPNGLALGLEKGDLFAKVITESEEKIE
                     TGKTFIFYTDGFTEAVNKRGDEYGLDRMFEIAKSWNYASANEIQDKMMADVNKFIGKA
                     PQHDDMTMVVLKIN"
     gene            9354..>9616
                     /locus_tag="VF870_13445"
     CDS             9354..>9616
                     /locus_tag="VF870_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX9253244.1"
                     /translation="MSSRKKVKFIFVTGGVVSSLGKGITASSLGLLLKRRGFRVTIQK
                     FDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFLDVN"
BASE COUNT         3115 a         1539 c         1619 g         3343 t
ORIGIN      
        1 taaattaaag gcgtcttaaa gacgcctttt taattttaat aattttaaaa tttccagcct
       61 actaatcatc ttaatgtgat ttttttcaat atatttaatt agtatttttg gctagtaaca
      121 ctttaatcag gcataaaaaa tttcattgaa atgatggcaa agaataaaaa aagagaaaga
      181 aaattcctac ctgtctttat tattctagct attacagcag atttggcctt tctatacttc
      241 aataagtata ataatcaaaa cttaacactt cgtgatttcg accttttcta ttttggtaat
      301 attataaatc tgttgtttgt acttcttctt ataattggac tcctggttat cattttcaaa
      361 aaatcaattg tatttaatct gaaaatattt atcccagtct ttctaataaa tcaatttata
      421 ttagttactc tttatttttt aaagtacatg cctttgccgt ttaaaaagtt atattatctt
      481 gggcagaccg gcgatgaatt attcatcggt gctatttttt tcttgtatac gctaacattc
      541 ctgatcctga tatttatagt ctggttaagc atttttaaat ttaagaaaat catacttttt
      601 agagcattta taaattctat tttatcaatg atagtaatcc ttttattggt ttttcttttt
      661 ataatcggaa aagaatcaac tatcaaggaa agcttactta aaattaataa aaataccgtt
      721 ggagtagttt taggcgctgc tgtgtggtcg gataatagac caagtccatc tctcgcagga
      781 agagttgaca aagctttaga gctttatcaa caaaaaataa tttcacaaat atttcttacc
      841 ggaagtaatg ctcctggtga attatctgaa tctgaggtag cgttaaacta tatcaaatca
      901 aaaggtatta aaacttcaga tatttttctt gagaaagcaa ctacttccac aaatgaacaa
      961 attcagtata taaaaaaaaa attagttgca actgcaaata aaaatattat tgtgatctct
     1021 gatagttatc atttagttag agttttggaa atagctaaat ttaacaagtt aaatattcag
     1081 gtttcggcat ctaaactttc tcagagtttt gataaaacta tatacaataa cacaagagaa
     1141 gctttagcat taactatgtt ttggctttat gcattataaa tggaagttaa gcactttcag
     1201 gagaaaaaaa tgtccacagt ttttaagcga agagttgtaa gagaaaaagt actgcaaatc
     1261 ctttatgcgt atgaaatgaa cgacgaaaat ttaatggctc ttactaagag tattttaggc
     1321 gatgttgaag aagagcagga tagaaagtat ggtgaagatc ttgttaacag ggttctaatt
     1381 cataaaaaga atcttgatac aaaaatagaa gcgagagtta gtaactggga aatgggtaga
     1441 atagcgctca tagataaaat acttctacga atggcaattt gtgagttgct gtattttccg
     1501 gatatccccc caaaggtttc aatcaacgaa gcgatagaaa ttgcaaaaga gtttagtact
     1561 gccggcagcg gtaaatttat taatggaatt cttgatgcaa ttttatcgga agaaaaaact
     1621 gcaggcagat taattaaaac cggcagaggt ttggttgatg aaacaatttc taaaaatcat
     1681 tccaaatctt ctgaatgaca gataagaaaa aaatatttac ctggactcta ttcgactttg
     1741 ccaataccgc tttttccgta ataatcgtaa cagttatata ttcaaaatac ttttcaaatt
     1801 acgtagcgga tggaagaaaa tggatttggg gtcttgcaat tagtctgtct atggtaatag
     1861 cagcctttct tagtccgcct cttggcgcta ttgcagacgt ttctagaaat cgaaaaagat
     1921 ttcttcttat ctttacatta gtttctgttg tatgcacggc gctgatgttt tttgttcaga
     1981 agggcgatat tctttttggg tttttattat tcgtttttgc caatgtagga tttgaagccg
     2041 gaatagtttt ttatgatgcc tttttgccaa acctaacaga aaagaaaaac tttggcaggg
     2101 tttccggtta tggttttgcc atgggttatg ttggcgctct tgtggtacta ttaatagtaa
     2161 tggttttgct tccggcacaa tctgacccta attattattt ttatattaga ttatctttta
     2221 ttgtagctgc tgcattcttt ttgatttttt ctatcccgtt gtttttattt ttaagagagc
     2281 ccaaaataga agatccagat aaaaaggatc ttgttaaaaa tggaataaaa aaaagcgttc
     2341 atactatcaa ggagattttt atacgtggcg gttatccctc tataaaacgt ttcctaattt
     2401 cattcttctt atataacgat ggaataataa ccattattgc tttcgcttct atttttgccg
     2461 ccaacgctct taaaatgact gatgcagaag taatatattt ttttgttata gttcagagta
     2521 cggcaatttt aggctcattt atttttggaa taatatctga ccatatcggc cctaaaaaga
     2581 caattaccat aactctattt atctggattg cagtaataat cggaaccttt tttattaata
     2641 caatttttga gttttatatt atcggtttga tggccgggtt atccattggt tcttcacaat
     2701 catccagcag aagtttgatg gctcttctta ttcccaaaga tagagaagct gagttttttg
     2761 gattttatga cgggctattt ggaaaatctt ctgcaatagt tggccctctt ttttatggca
     2821 tcatcgcaga cttgtctaat gagagagttg ccgctctttc catcggttta ttctttattg
     2881 ctggattaat tattttgcag aatgtagaag ttccggaatt cagattgtta aaaaaaaata
     2941 aagtatgatt tactctttaa tactggctaa acattacttc catttgtaaa tcggctcgcc
     3001 tgtaagttta ttaacattcc attcaaaaga tttagttttc tcttcacttc taacatcaca
     3061 ttgagcataa ttacaaaaac tgcataactg tgggatgcaa taatccagat gaattttaag
     3121 atcagcctgg ggaaaatctt tttgtaattc agattcaatt atgttttctt cttcatgaga
     3181 ttgcttgata ttaaaatagt atggaagtat aagatgaaaa tctataaata ctttatcacc
     3241 agattgccaa aaccgtaatc cgtgaatatc aatccaatag tcttttttta ttgaaaccaa
     3301 tttggctgaa agctgattaa gcatttccgg tttgacttca ttcattaacc cggcaataga
     3361 tccacgaatt aatttgtacc cggttaataa aatattaagt gcaatgatta ttgcaattat
     3421 tggatcaaga atttgaatat ctgtaataat aaccagcact actgcggtga taacaccaaa
     3481 acttgtaatg gaatctgtaa gtacatgttt cccatcagca attaaagtta aagagttagt
     3541 tttttttcct gtacggatta aaaagtttcc aagcagtaaa ttaaccactg aagcaaaagt
     3601 tatcacaaca gcaccaatgc ctaattgctt tagttcaggt ccagcgatta ttgcaaccac
     3661 tccctcgtaa atgataaaca ctgccgcaat tgcaattaat aaaccttcta taccagcaga
     3721 aaaatattca acattcccgt gtccgtaagg atgaaattca tctgcaggtc tggaacttaa
     3781 aataatgctg tataaagcca ttgaagtagc cataacgtgt acaacggatt cagcagcatc
     3841 agaaaaaatc gctgcggatc ctgttatcag ataggcgcca attttaaaga agaacatact
     3901 aaatcccaca acaagagaga taattgcagc tctttttctt atcgcaagtt gatctgattt
     3961 atgaatagtt gcattcatca gtgttacgga ctgtctaatt tttcagcggc ttgttttaaa
     4021 acttcatctt ttttagcgaa gcagacacga atcaatttat tgtcacttcc atctttataa
     4081 aaaggtgaaa gaggaattac ggcaacaccg atttctttgg tgagatattc agaaaattct
     4141 aattcgttct tatttgaaac cttagtgtaa tctaaaagct gaaagtacgt tccttcgcat
     4201 ttttttaaca taaattttga attcttcaga agcttagcga ataaatctct ttttgcttga
     4261 taaaaatctg ggagtgaata aatgttgttt tcatctttta aaagctctgc aacagctaat
     4321 tggatcggag tgtttactgc aaaaactatg aattgatgaa atttcctaaa ttctgcggtc
     4381 agattttccg gggcacagca gtaacccatc ttccagcctg tggtgtgaaa tgtttttcca
     4441 aaagaagaaa ttataaaact tctttttgct aattcatcgt aaagtgcaaa actgcaatgc
     4501 ttttgttttt caaaaatgat atgctcataa acttcatcgc ttatcagaaa aatatttgtc
     4561 ccctcaacaa tcttttttaa ttgggtaata tcatcttctt ttaaaattgc tcctgttgga
     4621 ttgtgaggag aatttattac aatgcactta gttttttttg ttattgattt ttcaactgta
     4681 ttccagggaa tgctaaaatc atttgtgtta agcttgattg aaattgggat tccgccattt
     4741 gtggttattg ccggcaggta tgaatcatac gcaggttcaa agattattac ctcatcacct
     4801 ttatttacga ttgcagaaaa agcagtataa agtgcttgag tagcgccggc agttactgtt
     4861 atttcttttt cataatcata ttcgcgtcca taatcccttt ttacttttat agctatttgt
     4921 tttctaagtt caactgtgcc cggcattggt gcgtattgat taaaacctga tttcataaaa
     4981 tgattaacga gttcgaccaa atgctccgaa caattaaaat ccggaaaccc ctgcgaaaga
     5041 ttaattgcat tgtaatcgtt tgccatttta gacataacag caaaaatact tgtgcctaca
     5101 tccggcagtt ttgaacttat tcctgctttg ctaatcataa ataaatttaa ttaacattaa
     5161 atgttttatc taggggcctt agattatcgt acaatcgttt taactgctca tatgcatatt
     5221 gattaccacg ttgtgcagaa ttccgataaa actctacagc acttggcaaa ctctttttta
     5281 caccgatccc tttttcataa caatagccca atgcaaattg cgcaagaata gaaccgtttc
     5341 tctcggaagt ttccaatatt gaaatctcat ttttcaaatc tgcactttgg tcagactcaa
     5401 aaacttttga cacagcaatt cttactgagg cttcctgact gttataattc tcggcaagtt
     5461 tccaatactc caaacctttt tgtttgtcat ttaatccata tttaccggta tagtaattaa
     5521 aaccaagttc tatgattgcg gggatatggt tttgtgcagc ggctttctgc aacaaattaa
     5581 tggcatcttt ttctgttaat cgattatcat atccaagttt ttgtagtccg taccaaacaa
     5641 actgtgccgt cggatcgccg agttgtactg catcatttaa aatagtatag aaatcttttt
     5701 cacgaataag tttccagagt aaatatgcgg ctctcggtga atctaatatg gttgctctta
     5761 tataatactc ggcggcaaat aatttgttaa tgccgacaaa attacccgtc tcataaattc
     5821 tgcctaatat tgtttctgct tcgtgacttc ctgattctgc aatttttcta agcagattga
     5881 tttttgttgc atcgaaaaag gaagttaaag aagaatcgtc tttaataact aatgaatcct
     5941 ttacaacctt tagatttaaa ttttttaaat ctgatatcag catcgcatct gtaatttctt
     6001 taatcgtatc tgattgcgta tcaaaatcta taaagaccaa accaagcgag ggctgtatct
     6061 ttgaatcctc tttatcaagt ttcttaaaag tatcttttgt taaattatat ttattctgat
     6121 caacagaatc tctgaaagca ggagagactt taggtgatat ctctgccaga agctctttag
     6181 caggcttata accaccctcg gcagactttt taatccaata gtaagattta ttccaatctc
     6241 tttgcacaat aagattatct gtatgaagta taccaaccac atactgcgcc tgactcattc
     6301 cagcttcagc agcaagcaag aaattatcat atgcagtaaa cggattccaa tccgtacccc
     6361 agccgttgat gagcattatc ccgtaattat aaagtgctga agtgagtttt tgtgatgctg
     6421 cttttttaat ccaataaact gcagcagcag tatcagccgc gattccttca cctgtcagta
     6481 atcttaaacc aagttcgtgc tgagctaatg gatctccgct gttagcaatc tggactaatt
     6541 taaattgctg taatatctga taggataaat caggttgata aaagtaagga ttatttctgt
     6601 atggtctgtt attcttaaaa gccagacttc tagttgtatc ttgtgcttca agtctgtttg
     6661 gaataaacaa aaaagaaatt aaagtaagta aaaaaggaat tagtattttt gatggcaacg
     6721 ttttctcgaa ctttcaatat gttacattaa acgaaatttt attctattat tgtgtgtaaa
     6781 aataaagttt atttgcataa ttatttagtg attttctaat ctaattaaaa gcataaatat
     6841 gatagataaa tcaaaacaaa tcatcattcc tttaattgtt ctaatctttg ccattattgt
     6901 aatctatttc ataataccag agtatcatcc atttggggga ttaagaattg ttaacaatca
     6961 agagattata ttaaaaaaag ctgaagattt tcttaacaaa tcgaatatat tatatgatga
     7021 aaatagactg aaaattaatt ttgatactga taagaatttt gtaaggtgga ttaattctga
     7081 gtataaaatt gatgaagcaa ataaaattct ttacaacgct ggttctgctt actactggac
     7141 ggtaagtcaa gataggactg acgatgccaa agtaattgta tcctctaatc ctggaagtaa
     7201 taacaatcgt aacaaatcta gttttgttct aaatattttg gataatggaa agctcatcgg
     7261 gtttagtaca gaacttgctg atacctctgt taaaaaatca ttatcagatg atggagcaaa
     7321 aaaattagcc gagaaattta taaaaggact ccgtgctgat attgtatttg tcgatgatac
     7381 acttcaatcc agtaacggaa taaatgccag tacattttac ttcaaagaaa cagaaaccaa
     7441 taataaacct gatcgcattg actataattt tacctggcaa acaaaaaata aaacaacgat
     7501 taattattat ctgaaagctc aggttgttgg tgatcaggta tcaaactttt caatccaggt
     7561 tatgattccg gatgaataca aaaagaatga aaatgatgtt tttgaaatag caactaccat
     7621 catatttatg cttctaattg ttatttccgt tattgtagtt ggatttaaaa gattcagagc
     7681 ttttgaagta ggatttaaac aagccattgc ttttggaatc tttgtattga tttcctttat
     7741 tgtaaaagaa ttacttgaaa gatttagctc ttttgaatgg acaataattc ttggacttgg
     7801 actgggaggt atttttattg caggggccgc aataattctt tgggcagtaa gcgaaactgt
     7861 cttcagagaa atctggaatg aaaaattctt gtcgcttgat ttaatctatc acagaaaaat
     7921 tgttcattca tctgttggta aagctataat aaacagtatt tcatttgggt ttggattaac
     7981 agcagtattt tttgttttac tctttgcgat cagttattat tcaaatgttt catttgcggg
     8041 tgaaaacttc atttcacaat cgcatatcac tgccaaattg ccattattaa atattttctt
     8101 tggtgtgttt aatagttttg gaattcttgc agttgctttc ttcatgttcc ttacagcagc
     8161 aatcaaaaga tatattaata atgatattat atttattctt gtaagcggtt ttacctggtc
     8221 attattaatg caatcgaatg tcaatcctct gacagctgga ctaccaataa accttgtaat
     8281 aggaatttta ttaagtctga ttctgattaa atatgatcta ttgtcaacgc tgttatcatt
     8341 tttacttttc aaattcttca ttaaagcaac tgagctgatg ttcatgactg aagccagtct
     8401 taaaaatgaa tggtatttac tagttgcaat ctgcttagtt cttattttaa tcggtataat
     8461 tctggttttt agaaaggata ctttcacaga ctatgattcc ataacaccaa agtttgttga
     8521 gaatattacc gaacgacaaa gattaaaaaa agaacttgat gttgcccgtc atgttcaaat
     8581 gagttttcta ccaaaagaaa atcccatctt aaaaggaatt gatattgctt cagtttgtat
     8641 tcctgctttt gaagtgggtg gcgattatta tgattttata tatcttggta aaaataaact
     8701 aggaataatt ataggagatg tttccggaaa aggaacacaa gctgcttttt atatgacatt
     8761 gacaaaaggt tttcttaaag caattgcaaa gaacaccgat tcacctgcgg aagtgttaac
     8821 taaaatgaat gaactgtttt atgaaaacgt tgagcggggc agatttattt ctatgattta
     8881 cgcagttgtg gatttagaaa ataaattgat tagaatcgca agggcagggc ataatcctgt
     8941 tatttatcat gattctgaag gaaacatcaa cctgatcagt ccaaatggat tagcactagg
     9001 tctggaaaaa ggtgatttat ttgctaaagt gattacagaa tctgaagaaa aaatagaaac
     9061 tggtaaaaca tttatttttt atactgatgg atttacagaa gcagttaata aaagaggcga
     9121 tgagtacggt ttagatagga tgttcgaaat agcaaaatcc tggaattatg cttcagcaaa
     9181 cgaaattcaa gataaaatga tggctgatgt taacaagttc ataggcaaag cgcctcagca
     9241 cgatgatatg acgatggtgg ttcttaaaat aaattaataa agtgtgtttt agttgaaaat
     9301 cagattggaa atcttagctg ctaactctat atttctaagc aaaaaaaagt aaaatgtctt
     9361 caagaaaaaa agttaagttt atcttcgtta ctggcggtgt tgtttcatcc ttaggcaagg
     9421 gaattacagc ctcatctctt ggtttattac taaaacgacg cggcttccga gtcacaatcc
     9481 aaaaatttga tccatatata aatgttgacc ccggaacaat gagtccgttt cagcatggtg
     9541 aagtttatgt aaccgatgat ggtgcagaaa ctgatcttga tcttgggcat tacgagcgtt
     9601 tccttgatgt aaatat
//
LOCUS       DASWBY010000742         8747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_626396, whole genome shotgun sequence.
ACCESSION   DASWBY010000742 DASWBY010000000
VERSION     DASWBY010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626396"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VF870_13450"
     CDS             complement(<1..982)
                     /locus_tag="VF870_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX9253245.1"
                     /translation="MNNKIKIPATPLAERIRPQTITDFYGQQHLLGKGKPIRLLIEND
                     TPSSFILWGPPGTGKTTIARIIANQTQAEFFQINAVSSGVKDIRAIIDIGSRNKEIGK
                     RTILFIDEIHRFNKAQQDALLHSIESGVLILIGATTENPSFEVIPALRSRARVFVLEE
                     LSKNDLVDILKSAIEKDEFLKQVKIKSVDEDYLIYLSGGDARIMLTILEAAVIQEIDK
                     TEIVLTKEIFENIIQRKNVLYDKGGEEHYNVISAFIKSVRGSDPDAALYWMARMLEGG
                     EDPLFIARRLLILASEDIGNASPNALVLAEAAFGAVDKIGMPEARIILAQC"
     gene            complement(1112..1411)
                     /locus_tag="VF870_13455"
     CDS             complement(1112..1411)
                     /locus_tag="VF870_13455"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX9253246.1"
                     /translation="MKFIENKKTRFYIIVSVVVIGTLYLLFNNYGVVKYAKVKSDLEE
                     LNTRITQLEEENRRLEAEIDSLKRNVPAKIEKIAREKYDMIRPNEKKIEFKAEEN"
     gene            complement(1408..2409)
                     /locus_tag="VF870_13460"
     CDS             complement(1408..2409)
                     /locus_tag="VF870_13460"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX9253247.1"
                     /translation="MDKKLKVLLLLGGTSPEREVSKATGKSVYNALINLGYEVVVIDP
                     AYGINQPFEVEDYFKETDYAEILNENYLDAVNLISSDQIDVAFLALHGKYGEDGTIQS
                     LLELKGIKYTGSKVLSSAIAMDKIMSKILFEEYHVPTPEYFHFKIGEHTTEEINTKIE
                     NQFGYPAVVKPNDQGSTVGLTICKSSDQLEEAIQNAFEYSDRILVEEYIPGRELTVAV
                     IDDIALPVLEIRPKHGIYDYECKYTSGMSEYIVPAEVSDEAAKELQEIAVQSCKSLRC
                     EVYARVDFRLSPENKVYALEVNTLPGMTSLSLVPKMAKAVGISFEQLVAKIINLSLK"
     gene            complement(2520..3173)
                     /locus_tag="VF870_13465"
     CDS             complement(2520..3173)
                     /locus_tag="VF870_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9253248.1"
                     /translation="MSLKKKLVEVCHKVYANGFVTAYDGNISCRTSSNTVLITRSGIC
                     KGDVTEKDIVEIDLDGKVLSKKQKISTEHKIHLYVYQKRKEVNAVVHCHPTYATAFAL
                     IGQGLDKHYLPEVFLTFGKIPLCKYATPSTEGVHKSLDPYINYAWAMLLENHGAVTLG
                     KNIEDAYYKMEKLEHAAKIIYLAKLIGKPKELSRKNINDILEIAESTYGIIPDKRNI"
     gene            complement(3152..4153)
                     /locus_tag="VF870_13470"
     CDS             complement(3152..4153)
                     /locus_tag="VF870_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9253249.1"
                     /translation="MVEKFYKGKKIKWGIAGCGRFAEQSFLPAVRLTRRSTVNSLYSR
                     DISRAKSLAEKFGVLNTFDNYDEFLKSDIDAVFVASANVHHYEQVIKAAKAGKNILCE
                     KPLALTSAQAEEMVKVCKDNKVLLAVNYVHRYHPLVIKAKELIVDQKLGKLVSIDVHF
                     NIDFPPDNNFRFKKELSGGGALRDIGTHMIDLLRFFGGEIKSIDGVVGNLVYQSEVDD
                     FAIGTVKFAKSGYGTFNVSFNNKKAFNRIEILCHKGAIAIDNLIGRKILAPKLTILLE
                     DETRKSFRKRGNKVHFVIRSVQKSFLKNEPPHASGQDGLINLKLMEELEQKCLSRKS"
     gene            complement(4158..5108)
                     /locus_tag="VF870_13475"
     CDS             complement(4158..5108)
                     /locus_tag="VF870_13475"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX9253250.1"
                     /translation="MKVIEHLEKAKNPLISFELIPPKRGGDIKGLLSVLDDITKYNPP
                     FIDITSHAAEVIYEETATGIKKKIKRKRPGTLGICALIQNKYNIDAVPHVLTKGFTRE
                     ETEDFLIELNYLQIQNVLAIRGDDSGYEKPIPEGKSKNPYASDLVKQIINMNNGKYLE
                     ESLLDARPTDFCVGVSGYPEKHFEAPNLKTDVKFVKEKVDAGADYVVTQMFYDNKYYF
                     KFVELCQELGIKVPIIPGLKILTSKANLTSIPKNFYISIPDALADEVMAAKPEHVIDI
                     GVEWAAKQVEELLNKNVPSVHFYIMQNSKPIIKLMERLKL"
     gene            complement(5276..7726)
                     /locus_tag="VF870_13480"
     CDS             complement(5276..7726)
                     /locus_tag="VF870_13480"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX9253251.1"
                     /translation="MRKWKKILIGLSSALVITFIIAGGIFYHMLSSSLPQYAGEIKSS
                     KIINDIEIYRDSFAVPYIIAQSDEDVAFALGYLHAQERLFTMDMIRRAGEGRLAEILG
                     EKAIPFDNMFRTVGIKRNIEKNLSLYDPAVMKILQAYSNGVNEYLKERKGNYAIEFDV
                     LGYQPEQWKPIHSLIVVKMMAWELNISWWTDLSFAELIQKLGKEKVLEILPDYPENAP
                     TIIADNFKFLPKINSTLAETDKAFRQMMGWTGTHIGSNNWVVNANKSVSGKPIIANDP
                     HLAFSAPGKWYAAVIKSKEWNAAGVTLPGVPGVVIGKGENISWVLTNVMNDDADFYFE
                     KLDSSKTKYFIDGQWQNLKIIKDTIKVKDGKDQVITIKETHRGPIISNIHPFKFIYNK
                     DDLDIQPISMRWSGNEFSDEMLAFYKLNKAKNWNEFRDAVKYFGIPGQNFVYGDAQGN
                     IGYIMGARVPIRKSNNPTLVFDGTNSENDWKGFVPVSEIHFILNPAENFIASANNKAL
                     KNFKYHISNLWEPSSRIDRIRELLTSKPKQSVEDFKKYQMDQVSPYARTIVPYVLKAF
                     EGIKVKDKNLNTSLELLSKWDFDLNKYSQTPAIYAVFLKFLLKNIYHDEMGDDLYNRF
                     VFLANVPYRSLLQILEKPESVWFNDVKTKKQETREEIIRKSLADALTYLEENYGKDLV
                     NWQWGRLHQVTFKHAFSGNFSLLDKYINIGPSEIGGDGTTLFNTEYSFAESIEEYSMF
                     RHKEFDNVLGPSMRFIFDFANPDEFYLILTTGESGNVMSENYRDQTPFWLNGKYMKIR
                     TDEESIRKNKNTLKIFRE"
     gene            complement(7807..8520)
                     /locus_tag="VF870_13485"
     CDS             complement(7807..8520)
                     /locus_tag="VF870_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9253252.1"
                     /translation="MSPEILTAFFVGLFGSFHCIGMCGPIAIALPVPNSNNLSFVTGR
                     LLYNLGRVTTYSFLGAVFGLLGSRLVISGFQQSVSIVLGIAIVIAVLIPPKYKVKITQ
                     HKLVQKITLPLKSGISDLFKRGTFSAMFLIGILNGFLPCGLVYVALAGAIASGDAISG
                     SAVMILFGLGTVPTMFAATIFGKFINLNIRRRITKLVPVFALVLGLLFILRGMGFGIP
                     YISPKISAQVVNESNLDCH"
BASE COUNT         2700 a         1682 c         1316 g         3049 t
ORIGIN      
        1 cacactgtgc tagaattatt cttgcttccg gcattccgat tttatcaacc gcaccaaatg
       61 cagcttctgc aaggactagt gcatttggag aagcgtttcc aatatcttca gaagcaagga
      121 ttaataatct tcttgcaatg aataatggat cctccccacc ttcaagcatt cgtgccatcc
      181 aatataatgc ggcatcagga tcactgccgc gaacactttt tataaatgct gaaataacat
      241 tgtaatgttc ttcaccacct ttatcgtaca aaacattttt cctttgtatg atgttctcaa
      301 atatttcttt ggttaaaaca atttcggttt tatcaatttc ctgaattaca gcagcttcaa
      361 gaattgtaag cattattctc gcatcgccgc cggaaagata aattaaataa tcttcatcaa
      421 ccgattttat tttaacctgt tttaagaatt catctttttc tattgcggat tttaagatat
      481 caactaaatc attttttgat aattcttcta aaacaaaaac tcttgctcgt gatcttaatg
      541 ccggtatcac ttcaaacgat ggattttctg tcgttgcacc aatcaagatc aatacacctg
      601 attcgatcga atgaagtaac gcatcctgct gcgctttatt aaaacggtga atttcatcta
      661 taaaaagtat tgttcgtttt ccgatttcct tatttcgcga tccgatatca ataatcgcgc
      721 gaatatcttt tactccgctt gatacagcat tgatctgaaa aaattctgcc tgggtttgat
      781 tagcaataat tcttgcaata gtggttttgc ctgttccggg aggtccccat aatataaatg
      841 aggatggagt atcattctca atcaataatc gaataggttt acctttgccg agcaaatgct
      901 gctgtccata aaaatcagta atagtttgcg gacggattct ttctgcgagt ggtgtggcgg
      961 gaattttaat tttattattc atatttataa gtcattcttc tgatcgtcag tcttagcgga
     1021 gacgaagact gacatatgca gtaggcacac ttcaacttcg ctcagtgtga ctattaaatc
     1081 acaagattac aaattttgta taatatggtt ttcaattttc ttccgcttta aattctattt
     1141 tcttttcatt cggtctaatc atatcatatt tttcacgggc aattttttca attttagccg
     1201 gaacatttct ttttagtgaa tcaatttctg cctctaatct tctattctct tcttcaagct
     1261 gagtgatccg tgtgttcaat tcttccagat cgctttttac ttttgcatac tttacaacgc
     1321 cgtaattatt aaatagtaaa tacaaagttc cgataacgac aacagaaact ataatataaa
     1381 atcttgtctt tttattttct ataaatttca tttcaaactt agatttataa ttttagcaac
     1441 aagctgctca aacgaaattc caactgcctt agccatttta ggaacaaggc tcaacgatgt
     1501 cattccaggt aatgtattta cttctaacgc atacacttta ttttcaggac tcaatcgaaa
     1561 atccacccgt gcataaactt cacatcttaa tgatttacaa gattgcacag caatttcctg
     1621 caattctttt gcagcttcat cagacacttc agcaggtaca atgtattcac tcatcccgga
     1681 ggtgtatttg cactcataat catatattcc atgctttggc ctgatttcaa gaacgggcaa
     1741 agcaatatca tcaattacag cgactgttaa ttctcttccg ggaatatatt cttcaaccaa
     1801 tattctatct gaatattcaa aagcgttctg aattgcttct tcaagttgat ctgatgactt
     1861 acagattgtt aatccaactg ttgatccctg atcatttggt tttactaccg caggataacc
     1921 aaattgattt tcaatctttg tattaatttc ttctgttgta tgctcaccaa tcttaaagtg
     1981 gaagtattca ggtgtaggaa cgtgatactc ttcaaacaat attttagaca taattttatc
     2041 catagcaatt gcgctggata aaactttgga gccggtatat tttattcctt ttagttccag
     2101 caaggattga attgttccat cttcaccata ctttccatgc agcgcaagaa aagcaacatc
     2161 aatttgatcg ctggaaataa gatttactgc atcaagatag ttttcgttca atatttctgc
     2221 gtaatcagtt tctttgaagt aatcttctac ttcaaatggc tgattgattc catatgcagg
     2281 atcaattaca acaacctcat atccaagatt tatcagtgca ttgtaaaccg attttccagt
     2341 tgcttttgat acttcccttt ccggtgatgt gccgccgaga agtaaaagta cttttaattt
     2401 tttatccatt tgaatcctaa aactttaatg tcacactgag cgaagacgaa gtgtgattgc
     2461 tctatattat aagggtcatc cttcgacttc gctcaggatg acaatttaaa tgattctgat
     2521 taaatatttc ttttatccgg aattattccg taagtgcttt ctgctatttc cagaatatca
     2581 ttaatgtttt tcctggaaag ttctttcggt ttaccgatta acttagctaa ataaattatt
     2641 ttcgccgcgt gttcaagttt ttccatttta taatatgcat cttctatatt tttgccaagt
     2701 gtaactgccc catgattttc aagcagcata gcccatgcat agtttatata cgggtctaaa
     2761 cttttatgca ctccttcagt tgaaggggtt gcatatttac acaaaggaat tttaccaaaa
     2821 gttaaaaata cttccggcaa ataatgctta tccaatcctt gtccaatcaa tgcaaaagca
     2881 gttgcataag tcggatggca gtgtacaacg gcattaactt cttttctttt ttgatacacg
     2941 taaagatgaa ttttgtgttc ggtggatatt ttttgttttt ttgaaagtac tttcccatct
     3001 aagtcaattt caacaatatc tttttcggta acatcacctt tacaaatccc ggatcgggtt
     3061 attaaaactg tgttactaga agtccgacag gaaatattac catcataagc agttacaaaa
     3121 ccattagcgt aaactttatg acaaacttca actaactttt tcttgagaga catttctgtt
     3181 ccagttcttc cattagtttt aagtttatca atccatcttg gccggaggca tgcggaggtt
     3241 cattttttag aaatgacttt tgaacggagc gaataacaaa gtgaacttta tttccgcgtt
     3301 tacgaaaact ctttctggtt tcatcttcca aaaggatagt aagtttggga gccaatattt
     3361 ttctgccaat caaattatca atggcgatag cacctttatg gcaaagaatt tctatcctat
     3421 taaatgcttt tttgttatta aaggaaacat taaaagttcc atacccgctt ttagcaaact
     3481 taactgtgcc aattgcaaaa tcatctactt cgctttgata aaccagattt ccaactaccc
     3541 catcaatgct tttaatctca ccgccaaaaa atcttagcaa atcaatcata tgagttccaa
     3601 tatcacgtaa tgctccgccg ccgcttaatt cttttttaaa acgaaaattg ttatccgggg
     3661 gaaaatcaat gttgaaatga acatcaatag aaacaagttt accaagtttt tgatcaacaa
     3721 ttaattcttt tgctttgata accaaaggat gataacgatg aacataattt acagccaaca
     3781 aaaccttatt atccttgcaa acttttacca tttcttcagc ttgagcagaa gttaaagcaa
     3841 gcggtttttc gcacaaaata ttttttcctg ctttagctgc ttttattacc tgctcgtaat
     3901 gatgtacatt tgcgctggca acaaatacag catcaatatc cgattttaga aattcatcat
     3961 aattatcgaa tgtatttaaa acaccaaatt tttctgcaag tgactttgca cgactaatat
     4021 ccctgctgta aagtgaattt actgtacttc ttcttgtcaa tctaactgca ggtaaaaaag
     4081 attgttctgc aaatcttcca cagccggcta ttccccattt aattttttta cctttataaa
     4141 atttttctac cattggatta cagttttaat ctttccatca attttattat cggtttagag
     4201 ttttgcataa tataaaaatg aacacttgga acatttttat ttaacagttc ttcaacttgc
     4261 tttgctgccc attcaactcc tatatcaata acatgctcgg gtttggcagc cataacttca
     4321 tcggccagag catcgggaat actgatataa aagtttttgg gaatagatgt taaatttgct
     4381 tttgaagtca atatttttag gcctggaata attggaactt ttattcctaa ttcttggcac
     4441 aattctacaa acttaaagta gtacttatta tcataaaaca tctgagttac cacataatca
     4501 gcccctgcat caactttttc tttaacaaac tttacatctg tcttaaggtt aggagcttca
     4561 aaatgcttct ccggatatcc gctgacacca acacaaaaat ctgttggtcg tgcatccaac
     4621 aagctttctt ctaagtactt cccgttattc atatttataa tttgcttaac taaatccgat
     4681 gcgtatggat ttttactttt accttccggt attggttttt cgtaacctga atcatcacca
     4741 cgaattgcaa gcacattttg aatctgcaaa tagttaagct caattaaaaa atcttctgtc
     4801 tcttctctgg taaatccttt ggttaaaaca tgcggcacag catcaatgtt atatttgttc
     4861 tggattaggg cacaaatacc aagtgttccg ggtctttttc ttttaatctt tttctttata
     4921 ccggttgcag tttcctcata tataacttcc gctgcatggg aggtgatgtc tataaaagga
     4981 ggattatatt ttgttatatc atcaagaacg gataatagac cttttatatc cccgccgcgt
     5041 tttggtggaa tcaactcaaa actaataagt ggatttttag ctttttctaa atgctcaatt
     5101 actttcatac attctcaaat tatttatgaa tacatttcta atataataat agttactgaa
     5161 aatcttgatg aatgcatatt acaaatgtca tttctaatct aacttcagtt ggtgggaaat
     5221 ctgcagtaag attccattcg tatctttcct gttggtcgaa ataactttca taaaattatt
     5281 cacgaaaaat ttttagtgta tttttatttt ttcttattga ttcttcatcc gttctaattt
     5341 tcatatactt accgttgagc cagaacggcg tctgatctct gtaattttca ctcataacat
     5401 tacctgattc acctgtcgta agtattaaat aaaattcatc cgggtttgca aaatcaaaaa
     5461 taaatctcat agatggtcca agcacattat caaactcttt atgccgaaac attgagtatt
     5521 cttcgatgct ttctgcgaat gaatattctg tattaaaaag agttgttcca tctcccccaa
     5581 tttcacttgg tccaatattt atatatttat caagtaaaga aaaatttccg ctaaaagcat
     5641 gcttaaaagt aacctggtgt aatctgcccc attgccagtt tactaaatct tttccataat
     5701 tttcttcaag ataagttaaa gcatctgcta aactttttct gataatttct tcacgagttt
     5761 cctgcttctt tgttttcaca tcattaaacc aaactgattc cggtttttct aaaatctgaa
     5821 gcaggcttct gtaaggtaca tttgcgagaa acacaaaacg attataaaga tcatcaccca
     5881 tttcatcatg ataaatattc tttaaaagaa attttaagaa cacagcataa atagcaggag
     5941 tttggctgta tttattcaaa tcaaaatccc atttacttaa taactcaagc gaagtattaa
     6001 gattcttatc ctttactttt attccttcaa aggcttttag aacatagggt acgattgttc
     6061 ttgcataagg tgaaacctgg tccatctgat actttttaaa atcctcaacg gattgctttg
     6121 gttttgatgt taacaattct cttatcctat caatacggga tgaaggttcc caaagattgg
     6181 aaatatgata cttgaaattc tttaaagctt tattatttgc agatgcaata aaattttctg
     6241 caggattaag gataaagtga atttcactta caggtacaaa tcctttccaa tcattttcag
     6301 aatttgttcc atcaaatacc agagtcgggt tattgctttt tcggatagga actcgtgcac
     6361 ccattatata accgatatta ccttgtgcat cgccataaac aaaattctgt ccgggaatac
     6421 caaaatattt tacagcatca cgaaattcat tccagttttt tgctttgtta agtttataaa
     6481 acgcaagcat ctcatcacta aattcatttc ccgaccatcg catactgatt ggctgaatat
     6541 ctaaatcatc tttgttataa ataaatttaa acggatgtat gtttgatata attggaccac
     6601 gatgagtttc tttgatggta attacctgat cttttccatc tttaactttt attgtgtctt
     6661 ttatgatttt aagattttgc cactggccat caataaaata ttttgttttc gatgaatcaa
     6721 gtttttcaaa ataaaaatca gcatcgtcat tcattacatt tgtaagtacc caggaaatat
     6781 tttcaccttt tccaattaca actcccggaa ctccaggcaa agtaacaccg gcggcattcc
     6841 actctttact tttaataact gctgcatacc atttacccgg tgcactaaac gcaagatgcg
     6901 gatcgtttgc aattattggt ttaccggaaa ctgatttatt cgcatttaca acccaattgt
     6961 tcgaaccaat atgtgtacct gtccatccca tcatctgccg gaaagcttta tctgtttctg
     7021 ctaaagtaga atttattttt ggaagaaatt taaagttatc agcaattatt gttggagcat
     7081 tttcggggta atccggtaag atttctaaaa ctttttcttt gccgagtttt tgtatcagct
     7141 ctgcaaaaga aagatcagtc caccaactga tattcaattc ccacgccatc attttaacaa
     7201 cgattaagct gtgaattggt ttccattgtt cgggttgata tccaagtaca tcaaattcta
     7261 tagcatagtt tcctttgcgt tctttcagat attcattaac accattagag tatgcttgca
     7321 aaatcttcat cactgccgga tcatacaaac taagattttt ttcgatattc ctttttatcc
     7381 caactgtgcg aaacatatta tcaaatggaa ttgctttctc gccgagtatt tcagctaaac
     7441 gtccttcacc ggctctgcga atcatatcca tagtaaataa tctttcctga gcatgcagat
     7501 aacccaatgc gaaagcaaca tcttcatcag attgagctat tatgtatggt acagcaaaac
     7561 tatcgcggta aatttctata tcattaataa ttttagatga ttttatttcg ccggcgtatt
     7621 gtggtaaaga agaggaaagc atatgataaa atatgccgcc ggcaattata aaagtaatta
     7681 ctaatgcaga tgataaacca attaatattt ttttccactt acgcataaat cttaattgaa
     7741 atgttatagg caaatatagg aaatattttg tagggattgg atttaaagga aacagctaaa
     7801 ctgaaactaa tggcaatcca aattggattc atttacaact tgtgcagaaa tttttggtga
     7861 gatgtacgga attccaaatc ccattcctcg taaaataaaa agtaatccga gcactaatgc
     7921 gaagacagga acaagtttag taattcttct tctgatgttg agattaataa actttccaaa
     7981 aattgttgca gcaaacattg ttggtacagt tcctaatcca aataaaatca taacggcgga
     8041 accggaaatt gcatcgccac ttgcaatcgc cccagcaaga gcaacataaa ccaatccgca
     8101 cggaagaaaa ccatttaaga tcccgattaa aaacattgca gaaaaagttc ctcgtttaaa
     8161 taaatcactt attccggatt ttaatggcag cgtaattttt tgaacaagtt tatgttgagt
     8221 tattttaact ttatactttg gcgggattaa gactgcaatt acaattgcaa ttcctagtac
     8281 gatagaaacg ctttgttgaa aaccagaaat tacaagtcgt gaacccagca acccaaaaac
     8341 tgcaccaaga aaggagtaag ttgttactct tccaagatta taaagcaatc gacctgtaac
     8401 aaatgataaa ttattagagt ttggaaccgg aagtgcgatt gcgattgggc cgcacattcc
     8461 aatacaatga aagcttccga ataatccaac aaagaatgct gttaatattt ctggtgacat
     8521 ttttaatctg taaaagaata aagaatctaa ttaaaagtaa aagtcatctc gaaggagtcg
     8581 tcgactgaga aatctaaatt actagaggga aaagatttct cccttcgatc gaaatgacta
     8641 ttactctttt cattcaattc aaaaatcatt cataatggtt tagttaatta cgaaagaact
     8701 ttctttataa tactcaaccg catcttgagt ccagttaagt ttaactt
//
LOCUS       DASWBY010000743         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_594557, whole genome shotgun sequence.
ACCESSION   DASWBY010000743 DASWBY010000000
VERSION     DASWBY010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594557"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..779
                     /locus_tag="VF870_13490"
     CDS             93..779
                     /locus_tag="VF870_13490"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9253253.1"
                     /translation="MLTKQKKLSRKEIKEDKLVEFYYKSISFFEGNKNKIFTYLGALA
                     VVVIAVILFMNYKSVQNDEAAVHLSKVMDMYDVGDYLGAIEGKKDVKVLGLKEIVSQY
                     GSTENGETAKIYLANSYANLGKPEEALKYYEDYSGDIDIYIAASLAGQASYYSVKNDY
                     EKAADLYMKASKVSKTDVMNSDYMFQAAVNYFDAGDKDQAKSLLQTIKEDYKTSAVYS
                     QVDRYLSQLN"
     gene            921..1484
                     /gene="efp"
                     /locus_tag="VF870_13495"
     CDS             921..1484
                     /gene="efp"
                     /locus_tag="VF870_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX9253254.1"
                     /translation="MADTSDFRNGLIIRYKNDPYVITEFQHVKPGKGGAFVRSTLKNL
                     KTGKVLDNTFRSGESIEIIRVERKKFQYLFRESEFLVLMDNETYEQINVPVVLFGDAV
                     DYLKGSEEIEILFNGDDIITVEPPIFVYLKVSETEPGFRGNTATNAFKPAKLESGATV
                     NVPLFVNVDDLLKVDTRTGEYVERVKS"
     gene            1500..1964
                     /gene="accB"
                     /locus_tag="VF870_13500"
     CDS             1500..1964
                     /gene="accB"
                     /locus_tag="VF870_13500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX9253255.1"
                     /translation="MDLNLIKKLIKILESSEVTDLEIEENGTRVKLAKKVRVTQAVSY
                     TQAPAASSVSQQISESGSKTEDKKLVDENAGMHEIKSPIVGTFYRAPAPDADSYVQVG
                     DTVSAGSVLCIVEAMKLMNEIESDVSGKIVKILVENGKPVEYNQPLFLVQIS"
     gene            1986..>2615
                     /locus_tag="VF870_13505"
     CDS             1986..>2615
                     /locus_tag="VF870_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9253256.1"
                     /translation="MFNKILIANRGEIALRVIRTCKELGIKTVAVYSEADRESLHVTF
                     ADEAVCIGPASGKESYLKIPSIISAAQITGADAIHPGYGFLAENADFSEICQESEITF
                     IGPSPEMIRAMGDKSFAKDTMRKNGVPVVPGSDGVVKDVSEAIKSAHEVGFPVIVKAS
                     AGGGGKGMRIVWEEKDFEKAFQNAQSEAEAAFSNGDVYIEKYLENPRHIE"
BASE COUNT          895 a          390 c          535 g          795 t
ORIGIN      
        1 ctgataatag atttttttaa tctttggaag attgggcact ttagtgttaa atttggccca
       61 gtttttttta tgaatcagaa taggaataag gaatgttaac aaaacagaaa aagctctcaa
      121 gaaaagaaat aaaagaagat aaacttgtag aattttacta taaatcaata tctttttttg
      181 aaggaaataa aaataagatt tttacttatt tgggtgccct tgcagttgta gtaattgcgg
      241 taattctttt tatgaattat aaaagtgtac agaatgatga agcagcagtt catttatcta
      301 aagttatgga tatgtacgat gtgggtgatt atcttggcgc cattgaaggt aaaaaggatg
      361 ttaaagtgtt aggactaaaa gaaattgtct ctcaatacgg cagtactgaa aacggcgaaa
      421 cagcaaaaat ttatcttgca aatagttatg ctaatcttgg caaacccgag gaagcattaa
      481 aatattacga agattacagc ggggatattg atatttacat tgcagcatct ttagctggcc
      541 aggctagcta ttattctgta aagaatgatt atgaaaaagc agcagatctt tatatgaagg
      601 catctaaagt ttcaaaaaca gatgtaatga attcagatta tatgtttcag gcagcagtaa
      661 actattttga tgcaggtgat aaagaccagg caaagagtct tcttcagaca attaaagaag
      721 actataaaac ctcagcggtt tattctcagg ttgatagata tctctctcaa ctaaattaag
      781 tgtttttgat agattttata taaaaaccgt gaagaaattc acggtttttt gtcaataatc
      841 gttaaaaagc ttttcttgac attacagact tctatctcta tttttttcaa gtcatttatt
      901 aacaattatt ttaaagtatt atggcagaca cttcagattt cagaaatggt cttattatca
      961 gatataaaaa cgatccgtat gtaattactg agtttcaaca cgtaaaaccc ggcaaaggcg
     1021 gtgcattcgt tcgcagtaca ttaaaaaatc ttaaaactgg caaagtttta gataatacat
     1081 tcagatcagg cgagtctatt gagattataa gagttgaacg taaaaaattt caatatcttt
     1141 ttcgtgaaag cgagtttctt gttcttatgg ataatgaaac ttatgaacag ataaacgttc
     1201 cagtagtttt atttggtgat gcggttgatt accttaaagg cagcgaagaa atagaaattc
     1261 tttttaatgg tgatgatata attaccgttg agcctccgat ttttgtctac ctaaaagtat
     1321 cggaaactga gcccggattt agaggcaata cggcaacaaa cgcatttaag ccagctaaat
     1381 tagaatccgg tgcaacagtt aatgttccat tgtttgttaa cgttgatgat ctgctaaaag
     1441 tagatacacg cacaggggaa tatgttgaac gagtaaaatc ctaataaaag gtataacaaa
     1501 tggatctaaa tcttataaaa aaactcatca aaattcttga atcaagtgaa gtaactgatc
     1561 ttgagataga agagaatggt acacgcgtaa agcttgctaa gaaagttcgc gttacacaag
     1621 ctgtatcgta tactcaggct cccgcagctt catctgtaag tcaacagatt tcagaatcag
     1681 gatcaaagac tgaagacaaa aaattggttg atgaaaatgc agggatgcac gaaataaaat
     1741 ctccaatagt tggtactttt tatcgggcac cagctcctga tgcagattct tatgttcagg
     1801 ttggagacac agtttctgca ggatcggttt tatgtatagt tgaagcgatg aaactgatga
     1861 atgaaattga gtctgatgta agtggtaaga tagtaaagat attagtagaa aacggtaaac
     1921 ctgttgagta caatcaaccg ttatttttag tgcagataag ttaatccaat aaacattgag
     1981 acgttgtgtt taataaaatt ttaatcgcta atcgtggcga aattgctcta agggtaatca
     2041 gaacctgcaa agaacttggc attaaaacag ttgctgttta ttctgaagct gaccgtgagt
     2101 ctttacacgt tacctttgct gatgaagcgg tctgcattgg acccgcctct ggtaaagaga
     2161 gttatcttaa aattccatca atcatttcag ctgcacaaat taccggtgca gatgctattc
     2221 accccggtta cggatttctt gcagagaacg ctgatttttc tgaaatctgt caggagtctg
     2281 aaattacttt cattggacct tcaccggaga tgataagagc aatgggtgat aaatcttttg
     2341 caaaagacac aatgaggaaa aatggcgttc cggttgtacc cggaagtgat ggcgttgtaa
     2401 aagatgttag tgaagcaata aaaagtgcac acgaagttgg ttttccggta atagttaaag
     2461 catctgcggg cggtggcgga aaaggaatgc gcatcgtttg ggaagaaaaa gattttgaaa
     2521 aggctttcca aaatgctcag tcagaagctg aagctgcttt tagcaatggc gatgtttaca
     2581 ttgaaaaata tttagagaat ccgcgccata tagaa
//
LOCUS       DASWBY010000744         3207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_67475, whole genome shotgun sequence.
ACCESSION   DASWBY010000744 DASWBY010000000
VERSION     DASWBY010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67475"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VF870_13510"
     CDS             complement(<1..420)
                     /locus_tag="VF870_13510"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX9253257.1"
                     /translation="MEENKNKVDKRVLLGSILIGLGGLFFLNSLDIFDFNFGRVIFSW
                     PFFFIVIGMYLTLNTSRKMLGGILAGLGFVFILPRIFPSINYDGSVVFAIFLIAIGSY
                     IILNQKKKTEQTDELGQLAQDVIDDVAIFGGGTKIVTS"
     gene            complement(582..1802)
                     /locus_tag="VF870_13515"
     CDS             complement(582..1802)
                     /locus_tag="VF870_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX9253258.1"
                     /translation="MKNHSKLDFETMCVHSGIDDYEYGSVVPPIYQTSTFKFKSAQHG
                     ASLFAGEEKGYIYTRMLNPTVQAMEDAIAALEGGHKALGCGSGMAAVHTIFAALTQAG
                     DHVVCSSAVYGPTTTLLNTIMKKFGVETTFVDTSNLDEVKKAMKPNTKVVYVETPGNP
                     TLCISDLTEISKIAHSNNALVVVDNTFMSPALQNPIALGADVVMHSLTKFLNGHADVI
                     GGIVIVKDEPTYINFRKTLNQIGGVIDPFNSFLVHRGLKTLALRMEKHCESAQIIAEW
                     LEKHPMIDSIRYPGLKSHPQYEIGLKQHKGPGGMITIELKGGIEAGRLMMNSVKLFQL
                     AVSLGGVESLIQHPASMTHFSMGKAARLDGGITDGLVRISVGIENVKDLIADLEQALK
                     KVEDAHLNEVEVHV"
     gene            complement(1914..>3207)
                     /locus_tag="VF870_13520"
     CDS             complement(1914..>3207)
                     /locus_tag="VF870_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9253259.1"
                     /translation="NTLFMIGSNTKAFTGTALAILESEGKLKLEDKVVQYLPDFKMKD
                     EWVTKELNLQDIVSHRMGMETFQGDFMYWTSDLTSDEVVEKFGKLTPKFDFRTKYGYT
                     NAGYAIAGKVIEKVSGLTWEDFLKEKIVNPLEMKRTVLLSEDFMKADNIAKPHTFVEG
                     KMTVLPFQNIDNLAPCGSIGSSINDLSHWIIAQLDSGNYNGINAIPFSSIQKTRQLLS
                     IERRVRHPYNKTHYTLYGMGWEFQDYEGREIVSHTGGVNGFVTSVTLLPEENLGIVVL
                     TNTDQNAFFQSLKWEILDSYLGLPYRNYDSTFYAGFLKRQDKNNSWLKEVRDSVAMNL
                     KPELSLSEFEGKYEHDVYGFSELKKVDDTLELTLEHHSKLKGKLKYIGNNRFLCTYSD
                     PTYGIKVFPFKIEDGIIKSFDFYVDDFIDYQAYKFIKQ"
BASE COUNT         1052 a          592 c          493 g         1070 t
ORIGIN      
        1 agaggttaca attttagtcc ctccgccaaa aattgcaaca tcatcaataa catcttgtgc
       61 taactgtcct agttcatcgg tttgttcagt tttctttttt tgattaagta ttatataact
      121 acctattgca attaaaaata ttgcaaaaac aactgaccca tcgtaattaa ttgatgggaa
      181 gattcttgga agaatgaaaa cgaaaccaag accggcaaga attccaccaa gcatttttct
      241 tgaagtgtta agtgtaagat acattcctat tacaatgaaa aagaatggcc aggaaaagat
      301 tactctgcca aaattaaaat caaaaatatc taatgaatta agaaagaata aacctcctaa
      361 tccgataagt atgcttccta gcaatactct tttatcaact ttgtttttat tttcttccat
      421 ttttctgctc cttaaataga tataccgttt tctggtggaa aactaataca aagataatga
      481 tagcgaaagt aaaaatcggc gaatggcagg taaacatcgg taattgatac taaaaataga
      541 agggaggttt aactcccttc tgttttaatt actttgaaaa actaaacatg aacttcaact
      601 tcatttaaat gagcatcctc aactttttta agtgcttgtt caagatcagc aataaggtct
      661 ttaacatttt caattccaac agaaatacga actaaaccat ccgtaatacc accatctaat
      721 cttgctgctt tgcccataga aaaatgcgtc atgcttgcag gatgttgaat caaactttca
      781 actccgccca aacttacagc aagttgaaaa agttttacag aattcatcat caatcttccg
      841 gcttcaattc caccttttaa ttcaatagta atcattccac ccggaccttt atgttgttta
      901 agtcctattt catattgtgg atgagatttt aaaccaggat aacgaattga atctatcatt
      961 ggatgttttt ctaaccattc agcaataatc tgtgcacttt cacaatgttt ttccattctt
     1021 aatgcaagtg tttttaatcc acgatgaacc aggaaagaat tgaaaggatc tataactccg
     1081 ccaatttgat ttagtgtttt acggaaatta atataagttg gttcatcttt aacgataact
     1141 attccaccaa taacatcagc gtgtccattt aaaaattttg ttaagctgtg cataactaca
     1201 tcagcaccta gagctatagg attttgcaaa gccgggctca taaatgtatt atcaacaaca
     1261 accaatgcat tgttactgtg tgcgatttta gaaatctctg ttaaatcaga aatgcaaaga
     1321 gttggattgc caggagtttc tacataaaca acttttgtat tcggcttcat agccttttta
     1381 acttcatcaa gatttgaagt atcaacaaaa gtagtttcga caccaaactt tttcatgatt
     1441 gtattaagta aagtagttgt tggtccataa actgcagatg aacaaacaac atgatcaccg
     1501 gcttgagtaa gtgcagcaaa aattgtatga acagcagcca ttccgcttcc gcagcccaga
     1561 gctttgtgtc cgccttcaag ggccgcaatt gcatcttcca ttgcctgcac agttggattt
     1621 aacatccttg tataaatgta acctttttct tcaccggcaa aaagtgaagc gccatgttga
     1681 gcagatttaa atttgaatgt agatgtttga taaatcggtg gaacaactga tccgtattca
     1741 taatcatcaa tacctgaatg aacacacatg gtttcaaaat ctaatttgga atgatttttc
     1801 atattttttc ccatataaca attaaataaa atatttagtg ctcaaaatta ggaaaaaatc
     1861 atgaaaggta tggaatggac ttttgcccat tccattttaa aaatctttat taattactgc
     1921 ttaatgaatt tataagcttg ataatcaatg aaatcatcaa cataaaaatc aaatgatttg
     1981 atgatgccat cttcgatttt aaatggaaat actttaattc cgtaagttgg atcagaataa
     2041 gtgcaaagaa atctgttgtt gccaatgtat ttgagcttac ccttcaactt ggaatgatgt
     2101 tcaagagtta gttcaagtgt atcgtcaact tttttcaact ctgaaaaacc ataaacgtca
     2161 tgttcgtatt tcccttcaaa ctctgaaaga ctcaattcag gttttaaatt cattgcaaca
     2221 gaatcgcgca cctcttttag ccaactgtta tttttgtcct gtctttttag aaatccagca
     2281 taaaaagttg aatcataatt tcgatatggc aaaccgagat agctatcaag aatttcccat
     2341 ttcaaagatt gaaaaaatgc attttgatca gtgttagtta aaacaacaat accaagattt
     2401 tcttccggaa ggagtgtaac agatgtaaca aatccattta cgccgcctgt atgagaaacg
     2461 atttctcttc cttcataatc ctgaaactcc catcccattc cataaagtgt ataatgtgtt
     2521 ttgttgtaag gatggcgtac tcttctttct attgataaaa gttgtctggt tttttgaata
     2581 gaagaaaatg ggattgcatt tattccatta taattaccgc tatcaagctg agcgattatc
     2641 caatgactta aatcattaat cgaagacccg atgctgccgc aaggtgcaag attatcaata
     2701 ttctgaaagg ggagaactgt catttttcct tcaacgaaag tatgtggctt tgcaatatta
     2761 tcagctttca taaaatcctc tgataaaaga acagtccttt tcatttcaag agggttgact
     2821 attttttctt taagaaaatc ttcccaggtt aatcctgaaa ctttctcaat tacttttccg
     2881 gcaatagcat agccagcgtt tgtataacca tactttgttc taaaatcgaa tttaggtgtt
     2941 agctttccaa acttttcaac tacttcatca cttgttagat ctgaagtcca atacataaaa
     3001 tcaccttgaa atgtttccat tcccattctg tgagaaacaa tatcctgtaa atttaattct
     3061 tttgtaaccc attcatcttt cattttaaaa tctggaagat actggactac tttatcttca
     3121 agttttagtt ttccttcgga ttcgagtatg gctaatgcgg ttccggtaaa tgctttggtg
     3181 ttagagccaa tcataaacaa agtgttt
//
LOCUS       DASWBY010000745         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_745195, whole genome shotgun sequence.
ACCESSION   DASWBY010000745 DASWBY010000000
VERSION     DASWBY010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745195"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..401
                     /locus_tag="VF870_13525"
     CDS             72..401
                     /locus_tag="VF870_13525"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX9253260.1"
                     /translation="MLKQFVLVIAVGFCLSVLSFAQDNTNKGLKDPHHKTETVNGSSK
                     TVSKEPWNKVCPVKGNPIEADSPTVEYDGKVYAFCCPGCDTKFAKNPEKYAKNLSEDG
                     KKFIGKK"
     gene            585..1001
                     /locus_tag="VF870_13530"
     CDS             585..1001
                     /locus_tag="VF870_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253261.1"
                     /translation="MKHKIITYTLISVFLFSVIGVPISLHYCDMMNLISLQSCGMCDL
                     KTSTCCDDDNSNPIVKSENDVSCCTNKIIAASSNEKYLTVIYEVQKTEAKSFVFATPK
                     QQYFTEELSNKTFSSDISPPLNYSNSIYLNNSILLI"
     gene            1092..>1509
                     /locus_tag="VF870_13535"
     CDS             1092..>1509
                     /locus_tag="VF870_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9253262.1"
                     /translation="MIEKIIEWSAKNRFIVILLYVIIVGFGIYSVINLPVDAIPDLSE
                     NQVIVFTEWMGRSPQIIEDQVTFPIVSGLQGLPDVKAVRANSMFGMSFVFVIFEDGVD
                     TYFARSRVLERMNSIQAQLPSGVVPSLGPDGTGVGHV"
BASE COUNT          511 a          226 c          270 g          502 t
ORIGIN      
        1 tattgaatta aaaatgtcac atttcgactt cgctcaatgt gacaattgta aaaaataaaa
       61 aggagtaaaa aatgttaaaa caattcgtgt tagtaattgc agttggattc tgtttaagtg
      121 tgttgagttt tgcacaagac aacacaaata aaggattaaa agatcctcat cataaaaccg
      181 aaacagtaaa tggtagttca aaaacagttt ctaaggaacc atggaataaa gtatgtccgg
      241 ttaaaggaaa tccaattgaa gcagattcac caacagtgga gtatgatggt aaagtttatg
      301 ctttctgctg tcctggttgt gatactaagt ttgcaaagaa tcctgaaaag tatgcaaaaa
      361 atttaagtga ggatggcaag aagtttatag gtaagaagta aagattctat tagtggtttc
      421 attccggacc ggataaccgg ttccggaatg aaataatttg ttaataaaag ctgctgtgat
      481 cagtctcata aatatcgggc agtaacaata aaaatagaat atttatatac tttcgattat
      541 tatttcattg cagtttgata attatttatt taggttactt aagaatgaag cacaaaataa
      601 ttacatatac acttatatca gtttttttat tttcagtaat tggtgttcct atatcactgc
      661 attattgcga tatgatgaat ttaatatcct tgcaatcctg tggaatgtgt gatttaaaaa
      721 cctcaacctg ctgcgatgat gataactcta acccaattgt aaaatctgag aatgatgttt
      781 cctgctgcac caataaaata attgcagcat cttctaatga aaaatattta actgttatat
      841 acgaagttca aaaaactgaa gcaaagagtt ttgtatttgc cactcctaaa cagcaatatt
      901 ttaccgaaga attatctaat aaaactttta gctcggatat ttctccacct cttaattatt
      961 ccaattctat atacctcaat aattcaatac ttctaatttg atatttcttt tcctagatta
     1021 gcttttaagg ataagtacat ccttttgcta tgtctattaa taatatttat taattgaatt
     1081 aggaaaagaa aatgatagaa aaaataattg aatggtctgc gaagaataga ttcatagtaa
     1141 tcctccttta tgttattata gttgggtttg gaatttacag tgtaattaat ttacctgtcg
     1201 atgcgattcc ggatctgtct gagaatcagg taattgtttt tacagaatgg atgggacgtt
     1261 cgccacagat tattgaagat caggtaacat tccccattgt ttccggatta caggggctac
     1321 ctgatgtaaa agccgtaaga gcaaattcaa tgttcggaat gtctttcgtc tttgtaattt
     1381 tcgaagatgg agttgataca tactttgcac gttcaagagt acttgaaaga atgaattcaa
     1441 tacaggctca gctaccttct ggggtcgtac cttcattggg accggatggc accggagtcg
     1501 gccatgtct
//
LOCUS       DASWBY010000746         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_710975, whole genome shotgun sequence.
ACCESSION   DASWBY010000746 DASWBY010000000
VERSION     DASWBY010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710975"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF870_13540"
     CDS             complement(<1..293)
                     /locus_tag="VF870_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253263.1"
                     /translation="MEWILIIIWLSIINGGLGGQYVLNWMANQPKYTGGSKVDVSPGV
                     MTWWREISKLAWVVVAVIVELIRGKEAKYLWPGFLSLTTIIICAFTGVIENIG"
     gene            429..1301
                     /locus_tag="VF870_13545"
     CDS             429..1301
                     /locus_tag="VF870_13545"
                     /inference="COORDINATES: protein motif:HMM:NF014951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253264.1"
                     /translation="MNKKAALLSTDNLEKFFTYDKLLIEPLKSIGWSAEEISWRSESV
                     NWNDYDAVIVRSTWDYQNNSEKFIKVLEKINNVSHLENNLELMKWNMNKNYLFDLEQK
                     GVKIVDTIWEKSFNSILAHQYFDKLNSEEIIIKPNISANADNTFRLSKENFNEHIPEL
                     EKIFASREFMVQPFLKNIIGEGEYSLFYFGGKFSHSVLKKPKEKDFRVQEEHGGDIQP
                     FAVSSEIIAIAESIIKKLFTIPLYGRVDLVRTNKKEFALIELELIEPSLYLNKDEQAP
                     NKFVRAFEERIANL"
     gene            1403..1987
                     /gene="hisH"
                     /locus_tag="VF870_13550"
     CDS             1403..1987
                     /gene="hisH"
                     /locus_tag="VF870_13550"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX9253265.1"
                     /translation="MVTIVDYGDACINEVAASLKKITDDFRISHNELDICKSDKLILT
                     GCGSAAAVMKKIQLLNLYSVLRVVKKPILGIGLGMQLMADHSTEGNFSCLGFFPGTAI
                     KIDDSIEESSYKGMHKINICRQSALFDGIDPNAEYYFNNSYYLPKSELSTTTCQSVVN
                     FCSSIENENAYGVQFHPEMSGEAGLKILNNFLEL"
BASE COUNT          738 a          310 c          334 g          638 t
ORIGIN      
        1 aaaccgatgt tttcaataac tccagtaaaa gcgcaaatga ttattgttgt taaggaaaga
       61 aaacccggcc ataaatactt tgcttcttta ccacgaatca gttcaacaat taccgccacc
      121 acaacccagg caagttttga aatttcccgc caccacgtca taacaccagg agaaacatca
      181 accttactac cgcctgtata ttttggctga ttagccatcc agtttaaaac atactgcccg
      241 ccaagcccgc cattaatgat ggaaagccaa atgataatta gaatccactc cacggaatta
      301 ttctcgctgg tttaattttt attttttatt tcgacccggc aaagttaaat aatttatatt
      361 tgtaaatcag ttattataat tattgctgat ataatcaata tttcaaatta acttaataga
      421 aaattataat gaataaaaaa gcagcacttc tctcaacaga taatcttgaa aaatttttta
      481 catatgataa gcttttaatt gaaccattaa aaagtatcgg ctggtcggct gaagaaattt
      541 cctggcgaag tgaaagtgta aactggaatg attacgatgc ggtaattgtc agatcaacct
      601 gggattatca aaacaattca gaaaaattta taaaagtttt agaaaaaatt aataatgtat
      661 ctcacctcga aaataatctt gagttgatga aatggaatat gaataaaaat tatctttttg
      721 atcttgagca gaaaggcgta aaaattgttg atacaatctg ggagaaaagt tttaattcta
      781 ttctggctca ccaatatttt gataaactta attcagaaga gatcattatc aaaccaaaca
      841 taagtgctaa tgctgataat acctttagat tatccaaaga aaactttaat gaacatatcc
      901 ctgaactgga aaaaatattt gcatcaagag aatttatggt gcagcctttt ttgaaaaaca
      961 taattggaga aggcgaatat tctctttttt attttggcgg aaagtttagt cattcagttt
     1021 taaaaaaacc aaaggaaaaa gattttcgtg tgcaggaaga acacggcgga gatattcagc
     1081 catttgcagt atcatctgaa ataattgcaa ttgctgaaag catcataaaa aaacttttta
     1141 caattccatt atacggaaga gtagatttgg taagaaccaa taaaaaagaa tttgcgctta
     1201 tagagctgga acttatcgaa ccctcacttt atttaaataa agatgaacaa gcaccaaata
     1261 aatttgtgag agcatttgaa gaaagaatag caaatcttta gcttcaaata aatgcgaaat
     1321 acttgatttg tattgttatt tttaaccaga agattatttt gacttataaa agttatttaa
     1381 aaacagaaat ttatttttac aaatggttac aatagtagat tatggtgatg cctgcatcaa
     1441 cgaagttgcg gcatcattaa aaaaaataac ggatgatttt agaatatccc ataatgaatt
     1501 ggatatatgt aaatcggata aactaattct tacgggttgt ggaagtgctg ccgcagtaat
     1561 gaaaaaaatt caacttctta atttatactc ggtgcttcgt gttgttaaaa aacccattct
     1621 gggaattgga cttggtatgc agctaatggc agatcattca actgaaggaa atttttcttg
     1681 tcttggcttt tttcccggaa cagcgattaa aattgatgat tctattgagg aatcttctta
     1741 taaaggaatg cataaaataa atatttgcag gcaaagtgct ttgtttgatg ggattgatcc
     1801 aaatgcagaa tactatttta ataattcata ttatttgccc aagagcgagc tatcaactac
     1861 gacttgccaa agcgttgtaa atttttgctc ttctattgaa aatgaaaatg cttatggcgt
     1921 tcagtttcat ccggaaatgt ctggtgaggc tggattaaaa atacttaaca actttttaga
     1981 attatagttg atgaatacag aaataaacac aaactcaatt
//
LOCUS       DASWBY010000747         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_106409, whole genome shotgun sequence.
ACCESSION   DASWBY010000747 DASWBY010000000
VERSION     DASWBY010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106409"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF870_13555"
     CDS             complement(<1..237)
                     /locus_tag="VF870_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX9253266.1"
                     /translation="MIEEKKNITKFQSWILASRPRTLPAALVPVMVGSALAIYQGIFF
                     PAYSIIALLCSILIQIGTNFTNDLYDHLKGSDTKE"
     gene            complement(237..1070)
                     /gene="menB"
                     /locus_tag="VF870_13560"
     CDS             complement(237..1070)
                     /gene="menB"
                     /locus_tag="VF870_13560"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX9253267.1"
                     /translation="MKSKWIKAKDYKDIIYEKLDGIAKITISRPEKRNAFRPETVFEM
                     YDAFSDAREDQTIGVILLTGQGPAKDGKYAFCSGGDQSIRGDMGYVGKDGVPRLNVLD
                     LQKLIRSIPKVVIALVAGYAIGGGHVLHVICDLTIAADNAIFGQTGPKVGSFDGGFGS
                     SYLARMVGQKKAREIWYLCRQYNAQEALDMGLINKVVSVDKLEEEGIQWASEILQHSP
                     MAIRVLKSAFNAELDGQSGIQELAGNATLLYYMSEEAQEGKKAYNEKRKPNFKKFPKL
                     P"
     gene            complement(1180..2001)
                     /gene="menH"
                     /locus_tag="VF870_13565"
     CDS             complement(1180..2001)
                     /gene="menH"
                     /locus_tag="VF870_13565"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HEX9253268.1"
                     /translation="MKLDANGVKINLEHLKEIDKTKDYVLFLHGFTGCAEDWFPVFEQ
                     MPDKYNCIAMDIVGHGKSDAPGNVVNYNIESIINQIKFVKDHLTPNKIFLLGYSMGGR
                     IALSYASVYPEDLKGLILESSSAGIKNDEERQKRYEEDLKIAEFIETHTLEEFIEMWQ
                     DQELFNTQRRFSNDKLKKFKKKKASGSKIGYANSLKGFSTGIMPPVHDKLKKIPLKVL
                     LITGDLDSKFTGINARLAKRFFKAKHKIVRNSGHNTHLEDSKRFIEIVLNYLGQF"
     gene            complement(1991..>2844)
                     /locus_tag="VF870_13570"
     CDS             complement(1991..>2844)
                     /locus_tag="VF870_13570"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9253269.1"
                     /translation="LEDYYSAVKPKGFAINKFGDTHNPSRRFKVIKANEELFLENLVN
                     SADLKQKDFSPELKILKNLNSEIEKLKPEIFSIAKKINETGVLLNIIDSIPPGSNLMI
                     GNSIPVRDLDFFASAVNKNINVFQNRGASGIDGITSTALGICAPSKNPTYLVTGDLSF
                     YYDINSLLIANQYSIPLITILINNNGGAIFKFLPIAEYKNVFEKYFLTSTNLSFKKLC
                     DAFEIDFKELKSQSDILNHIKVSSVRKKPAVFEIKTNADYSLSLRKKYWKKSNKIVEN
                     FLRNYET"
BASE COUNT          863 a          546 c          428 g         1007 t
ORIGIN      
        1 ttctttagta tccgaaccct ttaaatgatc atataaatca tttgtaaagt ttgttccgat
       61 ttgaattaag atggaacata acaatgcaat tattgaataa gcaggaaaaa atattccttg
      121 ataaattgct aaagccgaac ctaccataac cggaactaac gctgcaggta gtgttcgcgg
      181 tctgctggct aaaatccagc tttgaaattt tgtaatattt tttttctctt caatcattaa
      241 ggaagcttag gaaacttttt aaaatttggt ttacgttttt cgttgtatgc ttttttaccc
      301 tcttgtgctt cttcactcat atagtaaagt aaagttgcat taccggccag ttcctgaatt
      361 ccggactgtc cgtcaagctc tgcattaaat gcagatttta aaacacgaat tgccattggg
      421 ctgtgctgca aaatttcgct tgcccattga atgccttctt cttcaagttt atcaacgctt
      481 acaactttat ttattaatcc catatccaaa gcttcttgtg cattgtattg tctgcacaga
      541 taccagatct ctcgggcttt cttttgtccc accattctcg caagataact tgaaccaaat
      601 ccgccgtcaa aacttccaac ctttggcccg gtttgtccga agattgcatt atctgctgca
      661 attgttaaat cacaaattac gtgtagaaca tgaccgccgc caatagcata tcccgcaaca
      721 agtgcaataa caactttagg aatgctgcgt attaattttt gtaagtctaa aacatttaat
      781 ctaggcacac catctttacc cacataaccc atatcaccgc gaatgctttg atcgccaccc
      841 gaacagaatg catacttacc atctttggcg ggaccttggc ctgtgagtaa aattactccg
      901 attgtttgat cttcgcgcgc atcggaaaat gcatcgtaca tttcaaaaac tgtttccggg
      961 cgaaaagcat tccttttttc tggccggctg atagttatct ttgcaatgcc atccagcttt
     1021 tcgtagatga tatctttgta gtctttagct tttatccatt tagatttcat aaatgttcct
     1081 aaaaaaataa tcttcattaa aataaactta gttcagattt ctcaccggct aaacccggag
     1141 ccgagatgcc aggcatcagc tgattacaag ttaatcattc taaaactgtc ctaaataatt
     1201 taaaacaatt tcgataaatc gtttagaatc ttcaagatga gtgttatgcc cggaattacg
     1261 gacaattttg tgtttggctt taaagaatct ttttgcaagt ctagcattaa ttccagtaaa
     1321 cttgctgtca agatctccgg ttattaacaa aacctttaaa ggaatctttt tcaatttatc
     1381 gtgcacagga ggcataattc ctgtactaaa tccttttaaa gaatttgcat atccaatttt
     1441 actgccagat gcttttttct ttttaaattt tttcagcttg tcgtttgaga atcttctttg
     1501 agtattaaat aattcctgat cctgccacat ttcaataaat tcttcaagag tgtgggtttc
     1561 aatgaactca gcaatcttta aatcttcctc atatcgtttt tgtcgctctt catcattttt
     1621 aattccggct gaagaacttt ccagaatcaa tccctttaaa tcctcaggat atactgatgc
     1681 gtaacttagt gcaattctgc cgcccatcga ataacctaaa agaaaaattt tatttggagt
     1741 aagatgatct ttaacaaatt ttatctgatt gataattgat tcgatgttgt aattaacaac
     1801 attacccgga gcatcacttt taccgtgtcc aactatatcc atcgcaatac aattgtattt
     1861 atccggcatt tgttcaaaaa caggaaacca atcttctgca caacctgtaa atccatgcag
     1921 gaataaaaca taatctttgg ttttatcaat ttcttttagg tgttctaaat ttatctttac
     1981 gccattagca tcaagtttca taattcctca aaaaattttc tacaatctta ttggactttt
     2041 tccaatattt ttttcgtaat gataacgaat aatcagcatt agtttttatt tcaaatacag
     2101 caggcttttt tcttaccgaa gatactttta tgtgattcaa tatatccgat tgcgatttca
     2161 attccttaaa atctatttca aatgcatcac aaagcttttt gaaagaaaga tttgtagaag
     2221 ttaaaaaata cttttcaaaa acatttttgt attctgcaat cggaagaaat ttaaaaatag
     2281 cgccgccatt attattaatt aaaatagtta ttaatggaat gctgtactgg tttgcaatta
     2341 ataagctgtt tatatcataa taaaaagata aatcaccggt tacaagataa gttggatttt
     2401 ttgatggagc acaaataccc agcgcggtag aagtaatccc atcaattccg cttgcgcccc
     2461 tgttttgaaa aacattaata tttttgttta ctgcagatgc aaaaaaatca agatcacgta
     2521 ccggtatact gtttccaatc atcagattag agccaggtgg aattgaatca attatattca
     2581 gcagtacacc agtctcatta atcttttttg cgattgaaaa tatttctggt ttaagttttt
     2641 caatttctga atttagattt tttaatatct ttaattctgg tgaaaaatct ttttgtttta
     2701 gatctgctga attgacgagg ttttccagga acaattcttc atttgcttta atgactttaa
     2761 atctacggga aggattatgc gtatctccaa atttattaat tgcaaaacct tttggtttaa
     2821 cagctgaata gtaatcctcc agtt
//
LOCUS       DASWBY010000748         4226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_556417, whole genome shotgun sequence.
ACCESSION   DASWBY010000748 DASWBY010000000
VERSION     DASWBY010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556417"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..2571
                     /locus_tag="VF870_13575"
     CDS             145..2571
                     /locus_tag="VF870_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX9253270.1"
                     /translation="MNKLLLQILITILLIQPLISQERFPSYYSQNDMELSTPGTIIFG
                     LGGYINPATLSYLSQPNLYFTWNDQNSDFNDFNNFGLFASLPNFGFSLVDKKQNDYSI
                     TDYKLSAAFGSESFSLGIGYGWSSGDVGYYNRSNLFTLGAIYRPANFLSFSLIGNLPT
                     NNEREGIIGVGVRPFANYNLTFFGDYIFTQDTIPENITWSAGAIVEPIDGLRIVGRYF
                     EGKSFNVGIQLGFGILGFTTISHYDNESNQSHNTYGIRIGADDRNLLRIFSGNDNYVS
                     MNLQGGIKYQNFKLFDNSKTLFNLLKQLDAVENDKSVSGIAINLSGANINKEMYWELR
                     EKLREIKSHGKKVYIYIDRAGMDEYHFASIADKIVLDPMGTISLNGYIMGRTFFKGTL
                     DMVGLGFRELRYFKYKSASESYAREDFSEADREQRQKLVDDNYSLAQRDICEGRKFTP
                     SYFDKLVDSTFIFLPEDALNLKLVDTLARWSDISEIIKKYERENKNLISASSLKEFNL
                     PDDYWGSKPKIAVIYAIGGTGMDDGIKARNLVKYVEAAMNSGNVKAIVLRVDSPGGDA
                     LAADLIAEVLRKGKGKKPIIVSQGYVAASGGYWLSMYADTIVAAPNTITGSIGVIGSY
                     LFNKTLKQDLGLSVDHVQKGKFADLGFGATLPLIGISLPDRDFTDEELKMVETGIKAL
                     YKGFVDKVALGRKKSFDDIEKIAQGRVWSGSDGLRNGLVDVLGGLDTAIKIALQKSRL
                     TNKEYEIIEMPEREWFDLNAFLPSFLRIEQKIVEDPFIRDLKFRLQYKGIPMPMLPLE
                     FIDEGMIYEE"
     gene            complement(2666..3889)
                     /locus_tag="VF870_13580"
     CDS             complement(2666..3889)
                     /locus_tag="VF870_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX9253271.1"
                     /translation="MLKIKLHWQILIAFVLAIILGISFPEHSSYVRWLGDLFLRALKM
                     IIVPLVFSSIVSGVANLGSTSSLGRLGIKTITYYISTSVFAILTGLLLVDLIKPGLGA
                     DLGFRMDVPELTSSTGSLGDIILRMIPTNIFDALASADMLAIIFFAIMIGFFIPRVQN
                     EYSSFMINLFNAIFEVMMKLTSFIIKFAPIGILGIVTGVVADQAADKSKLLSMVQHLG
                     IYMVTVLAGLSIHFFITLPLILKFVGKTNPFLHFKAMSLPIITAFSTSSSSATLPLTI
                     EAVENNAGVSNKISSFVLPLGATINMDGTALYECVAAMFIAQAYGIELGFVQQIIVVV
                     TALLASIGAAGIPMAGLVMMSVVLTAVGLPLEGVGLILAVDRILDMCRTVVNVFSDSC
                     GAAVIAKSEGETLKV"
BASE COUNT         1416 a          672 c          784 g         1354 t
ORIGIN      
        1 ttattatgat ttatttacaa tgtctaatgg gataacagaa gtgtatgtaa atgagttccc
       61 aaagttttag gtcatttatt tatcccttta aatattaatt gtattatttt tcaagagaaa
      121 aaacttctga aagcggagat tgctatgaat aaactcttat tacaaatttt aataacgatt
      181 ttattaatcc agccgttgat atctcaagaa cgatttcctt cttattactc gcaaaacgat
      241 atggaacttt ctacaccagg aacaataata tttggtttgg gcggatatat taatccggct
      301 accttgtctt atttatctca accaaatctc tactttacct ggaatgacca aaattcagat
      361 tttaatgact ttaataattt tggacttttt gcttcattac caaattttgg ttttagtctg
      421 gtggataaaa aacaaaatga ttactccata acagattaca aactttcggc tgctttcggc
      481 tcagaatctt ttagtctcgg aattggctac ggctggtcat caggtgatgt tggttattac
      541 aacagatcaa acctttttac gcttggcgca atttatcgtc ctgctaattt tttatcattt
      601 agcctgatag gaaatcttcc gactaataat gaacgtgaag gtataatcgg cgttggagta
      661 cgaccgtttg cgaattataa cctgaccttt tttggcgatt atatttttac gcaggataca
      721 attcccgaaa atattacctg gagtgccggc gctattgtcg aaccgattga tgggcttaga
      781 attgttggaa gatattttga aggaaagagt tttaatgttg gcattcaatt gggattcggc
      841 attttaggat ttacaactat atcacattat gataacgaat ctaatcaatc tcataatact
      901 tatggaatta gaattggtgc tgatgataga aatcttttaa gaattttttc aggtaatgat
      961 aattacgtga gcatgaatct gcagggtggg atcaagtatc aaaactttaa gttgtttgat
     1021 aacagcaaaa ctcttttcaa tttattgaaa caacttgatg cagtagaaaa tgataaatca
     1081 gtttccggga ttgcaataaa tctatcaggg gctaatatca ataaggaaat gtattgggaa
     1141 ctgcgcgaga aactgcgcga aataaaatca cacggtaaaa aagtttatat ttatatagat
     1201 cgagcgggaa tggatgagta ccattttgca tccatagctg ataaaattgt tcttgatcca
     1261 atgggaacaa tttctctaaa cggatacatt atgggcagga cattctttaa aggtacattg
     1321 gatatggtag gtctgggctt tagagagttg agatatttta aatataaatc agcttcagaa
     1381 agttatgctc gcgaagattt tagcgaagca gatcgcgaac aaagacaaaa attggtagat
     1441 gataattata gtcttgctca acgggatata tgcgaaggaa gaaaatttac tccctcatac
     1501 tttgataaac tggttgacag tacttttatc tttttacctg aggatgcatt aaatcttaaa
     1561 cttgttgata cgcttgcaag atggagtgat attagtgaga ttattaaaaa gtatgaaaga
     1621 gaaaataaaa atttaataag tgctagctca ttaaaagagt ttaatttacc ggatgattac
     1681 tggggatcga aaccaaagat tgcagttatt tatgcaatcg gcggtacagg tatggatgac
     1741 ggaatcaagg ccagaaattt agtgaagtat gttgaagctg caatgaacag cggaaatgta
     1801 aaagcaattg tgcttcgggt tgattccccg ggcggtgatg ctcttgccgc tgatcttatt
     1861 gctgaagtat tgcgtaaagg aaaaggaaag aaacctataa ttgtttcgca aggttatgtt
     1921 gctgcttcag gcggttattg gctttctatg tatgctgata caatagttgc agcaccaaat
     1981 acaattacag gatcaatagg tgttattggt tcttatctgt ttaataaaac tttaaagcag
     2041 gatctcggac tttcagtcga tcacgttcaa aaaggaaaat ttgctgatct tggttttggt
     2101 gcaacgcttc cattgattgg tatttcactt cctgatcggg attttactga tgaggaattg
     2161 aaaatggtag aaacaggaat caaagccctt tacaaaggat ttgttgataa agttgcttta
     2221 ggcagaaaaa aatcttttga tgatatagaa aaaattgcac agggtagggt gtggtccgga
     2281 agtgatggat tgagaaatgg attagttgat gtgcttggcg ggcttgatac tgcaattaaa
     2341 attgctcttc agaaaagccg ccttacaaat aaggaatatg agataatcga aatgcctgaa
     2401 cgtgaatggt ttgatctcaa tgcatttttg ccgagctttt taagaattga acagaagatt
     2461 gttgaagatc cgtttataag agatttgaaa ttccgcttac agtataaagg aataccaatg
     2521 ccaatgcttc cgctggagtt tattgatgaa ggtatgattt atgaagaata agatttttag
     2581 ttaattgcac tttagcgtct ttgcgtaaaa tcttttttct caggcagaga cgtaaaagtt
     2641 tattttaaaa acattttaga tagtgctaaa ctttaagagt ttcaccttct gattttgcaa
     2701 ttactgcagc tccacagcta tcactaaaaa cattaacaac tgtgcggcac atatcaagaa
     2761 ttctgtcaac agcaagaatt aatcctacac cttctaatgg caatccaaca gcggtaagaa
     2821 caactgacat cattacaaga ccagccattg gtattcccgc tgccccaatt gatgccaata
     2881 atgcagttac aacaacaatt atttgctgaa caaatcctaa ctcaattcca tatgcctggg
     2941 ctataaacat ggcggcaaca cattcgtata aagcggttcc atccatatta attgttgcac
     3001 caagcggaag aacgaaactg gaaattttat ttgaaactcc tgcattattt tctactgctt
     3061 ctattgttaa aggtaatgtt gcagatgatg atgaagttga aaatgctgtt atgattggaa
     3121 gagacattgc cttaaaatgt aaaaatggat ttgtcttgcc gacaaatttc aaaatcaaag
     3181 gtagagttat aaaaaaatga attgataatc ctgcaagcac tgtaaccata taaattccaa
     3241 gatgctgaac cattgaaaga agtttagact tatctgccgc ctgatcagcg acaactccag
     3301 ttacaattcc aagaattcca attggggcaa attttataat gaatgaagtt aatttcatca
     3361 tcacttcaaa tattgcattg aataggttga tcataaaaga tgaatattca ttttgcactc
     3421 gaggaataaa aaaaccaatc atgatcgcaa agaaaattat agccagcata tctgctgaag
     3481 ctaatgcatc aaagatattt gtgggaatca tccttaaaat aatatcaccc agcgatccgg
     3541 ttgatgaagt aagttcaggc acatccattc taaatccgag atcagcccct aaaccgggtt
     3601 ttataagatc aacaagaagc aatccggtta gtattgcaaa cacacttgtt gaaatgtaat
     3661 aagttatagt tttaataccg agtctgccca aactgctggt actgccaaga tttgcaactc
     3721 ccgatacaat cgatgagaac actaatggaa caattatcat tttaagtgca cgaaggaaaa
     3781 gatcaccaag ccatcttaca taactggaat gctcgggaaa cgatatacca agaattattg
     3841 caagtacaaa tgcaattagt atttgccaat gtagttttat tttaagcata aattattttc
     3901 tattaattca agacttactt tttaatcatt atattattaa gagtcatttc taaggatggc
     3961 aaagccagac tgagaaatct gatttaacag aatattcaga tttctcactg cggtcgaaat
     4021 gacaagtcaa ttcgttttta tttattcaaa attaattata atatattctt taatatctca
     4081 tcacaaactg cataaccctt tgatgtaagt tttatccaat tattttcaat tgataaatgc
     4141 tgttcattct ttaataattc aaaatatgaa taattttttt caatccaatc attagaaaat
     4201 ctttttctat aatcgtttaa atccaa
//
LOCUS       DASWBY010000749         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_594860, whole genome shotgun sequence.
ACCESSION   DASWBY010000749 DASWBY010000000
VERSION     DASWBY010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594860"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VF870_13585"
     CDS             <1..1331
                     /locus_tag="VF870_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX9253272.1"
                     /translation="TADNEGEFYQLPAKGKHQILNLNYELAKSRASRNRKRVKQFLLE
                     GWEPSRDLKPLIDLTEEYLAEAFKMEQDETKCAQLSQSSLYYSMWAGEKIEVEKSWYD
                     IAKNKFRDNFFIGCDTKGFGKMDNELFLDLFSDLFNYATITHYLPSFQKEEGVYTYGN
                     RDEQFKLLKARGITVEGRPIFWADECCTPDWLMDKSFSEILKYVERHTREVVSHYGDE
                     MYAWEIINEAHDFGNIQKLLPDQLVQIAKLIAEVAKDTNPKVHRLINNCCINADYVQI
                     WTPKETYKSSQIVTPHQFIKMCYEAGVDFTITGQQLYYQYTNRDLADTIRMTERLEKF
                     GKPVQITEIGTTSGPTKETVASGKYELPNRPYSWHREWDQDLQADWLEQIYTIMYSKP
                     WVEAINWYDFVDPYSFIQNGGLLADTEGNKKEAYNRLKNLKEKWQSYAKK"
     gene            1373..2926
                     /locus_tag="VF870_13590"
     CDS             1373..2926
                     /locus_tag="VF870_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX9253273.1"
                     /translation="MSTQKGDLIFKPYFVQRGMGPNLLNWAYACDENWDAFYSNISSS
                     NDGVVISDTAGVEKFSVEVRWNLEDFGYIFISADNGGEFYELPPAGGKKELNLNFELA
                     KSRVFRNRRRIEKHKTGGWIPSYEVMSFVHLSEELFSDAERFKSNNDKCAELSQKCLL
                     YGMHASEKIELEKAKYEISKNKIRKDFFIGCDARGFYQMDPELFLELFTKQFNYATIT
                     YYQISGNYRDFEPTEGDLQFATRDVVYNELKKNNITIEGRPLYWPYKTVTPDWMRNKS
                     YDQLLKYIEKHTREVVGHYGEGMYAWEIVNESHDWANETQLTPEQITNITKLACEVAK
                     DTNPKVHRLINNCCPYAEYVQLKKWGDLDAKYPQRTPIKFMQDLVDNGVDFTISGQQM
                     YFPYRDLSDIIIHLERFEKFGRPVQLTEVGASSGPNKTSIDNGSLEISNEPYIWRRNW
                     DQELQADWLEELYTIAYSKSWIEAVNWYDFVDPYSWIKNGGLLESPKGEKKASYDRLL
                     KLQQSWGLK"
     gene            2927..>3314
                     /locus_tag="VF870_13595"
     CDS             2927..>3314
                     /locus_tag="VF870_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253274.1"
                     /translation="MSNGKIIFKPYFVHKGRGPHLFDFAMTLDHNGDAFHSDIKITSE
                     GITISDTEGRDKFSISARWNVEGYGYLFIPADNEGEFYSLPESGKIELNLNYEFAKTR
                     VSRNRRRKIKFEESGWTPSNELRHYLE"
BASE COUNT         1179 a          525 c          704 g          906 t
ORIGIN      
        1 tgacagccga taatgagggt gaattttatc aattaccagc aaaggggaag caccaaatac
       61 ttaatctgaa ttacgagctt gcaaaatcga gagcatcaag aaaccgcaaa agggtaaaac
      121 aatttttgtt agaaggatgg gaaccatcaa gagatctaaa acccctgatt gatttaacag
      181 aagaatatct tgctgaagct ttcaaaatgg aacaagatga aactaagtgc gcgcaattat
      241 cacagagttc gctttattac tcaatgtggg caggtgaaaa aatagaagtt gaaaagtcct
      301 ggtacgatat cgcaaaaaat aaattcaggg ataatttctt cataggatgc gatacgaagg
      361 gatttgggaa aatggataac gagttgtttc ttgatctgtt ttctgatctc tttaattacg
      421 ccacaatcac tcattacctc ccaagttttc aaaaagaaga aggtgtctat acatacggaa
      481 acagagatga acagtttaag cttttaaaag caagaggtat tacagttgaa ggacgtccaa
      541 tattctgggc agatgaatgc tgtacacccg attggctgat ggacaaaagt ttttctgaaa
      601 ttctaaaata tgtggaaaga catacaagag aagttgtaag ccattatggt gatgaaatgt
      661 atgcctggga aataattaat gaagcacatg attttggaaa tattcaaaaa cttcttccag
      721 atcaattagt gcaaatcgca aaattaattg ctgaagttgc aaaagataca aacccgaaag
      781 tgcaccgcct gataaataac tgctgcataa atgcggatta tgtacagatt tggacaccaa
      841 aagaaacata taaaagttca cagattgtaa ccccgcatca gttcataaaa atgtgttatg
      901 aagcaggagt tgattttact atcacaggac agcagctata ttatcaatat acaaacagag
      961 atttggcaga tacaattcgt atgactgaac ggcttgaaaa atttgggaaa ccggttcaga
     1021 taactgaaat tggaactaca tcagggccaa cgaaagaaac agtagcatcc ggtaagtatg
     1081 aattgcctaa tcgaccatac agctggcaca gagagtggga tcaggatttg caggctgatt
     1141 ggctcgaaca aatttacaca atcatgtaca gcaagccttg ggttgaagcg attaattggt
     1201 acgattttgt tgatccgtat tcattcattc agaacggcgg attattagct gatacagaag
     1261 gtaataagaa agaagcatat aaccgtttga agaacttaaa agaaaagtgg cagtcctatg
     1321 ctaaaaaata gatctacaga atttttaaaa cttaagaatt agcggagcca gtatgtctac
     1381 tcaaaaaggt gatctgattt ttaaaccata ttttgttcag cgcggaatgg ggcccaacct
     1441 tcttaattgg gcttacgcct gcgatgaaaa ttgggatgcc ttttattcca atattagctc
     1501 aagtaatgat ggtgtagtca tatcggacac tgccggcgtt gaaaaatttt ctgttgaagt
     1561 aagatggaac ttggaagact ttggttacat atttatctca gcagataatg gaggcgagtt
     1621 ctatgaactc cctccggctg gagggaaaaa agaattaaat ctgaattttg aactagctaa
     1681 gagtcgagtt tttaggaaca gaagaagaat tgaaaaacat aaaactggtg gatggattcc
     1741 ttcttatgag gtgatgtcat ttgttcatct ttcagaagaa ttatttagtg atgccgagag
     1801 attcaaaagt aataatgata agtgtgcaga actttcgcag aagtgtttat tgtatggaat
     1861 gcatgcaagc gaaaagatcg aactagaaaa agcaaaatat gaaatttcga aaaacaagat
     1921 tagaaaggat ttctttattg gttgtgatgc caggggattt tatcaaatgg atccagaatt
     1981 gtttcttgag ctcttcacaa aacaatttaa ttatgcgaca attacctact atcaaataag
     2041 cggaaattac agagactttg aacccacaga aggagatctt caatttgcaa caagagatgt
     2101 tgtatataat gaattgaaaa agaataatat aactatcgag gggagacctt tgtattggcc
     2161 ttataaaaca gtaacacctg attggatgag aaataaatct tatgatcaac tattaaaata
     2221 tatcgaaaaa catacgcgtg aagttgtcgg acattatggt gaaggaatgt atgcctggga
     2281 gatcgttaat gaatcacacg attgggctaa tgaaactcaa ctaactcctg aacagattac
     2341 aaatattacc aagcttgctt gcgaagttgc aaaggacact aatccgaaag tgcatcggct
     2401 gattaataat tgctgtccct acgctgaata tgttcagctt aaaaaatggg gtgatctaga
     2461 tgcaaaatat cctcagagaa cgccgattaa atttatgcaa gacctggtcg ataacggtgt
     2521 ggactttaca atatccggcc agcagatgta ctttccatat cgagatttat ccgatataat
     2581 tattcatctg gaaagatttg agaaattcgg gagacctgtt cagttaacag aagtaggtgc
     2641 atcatcgggt ccgaataaga cttcgataga taatggatcg ctcgaaattt caaatgagcc
     2701 gtacatttgg aggagaaatt gggaccagga actgcaagca gactggcttg aagagctcta
     2761 cactatagcc tacagcaaat cctggatcga ggctgttaac tggtatgatt ttgtcgatcc
     2821 atattcctgg attaaaaatg gaggtttatt ggaatcacca aaaggcgaaa aaaaagcttc
     2881 gtacgataga ttgttaaaac tacaacagag ttggggatta aaatagatgt caaacggtaa
     2941 aattattttc aaaccatact ttgttcataa gggcagaggt ccgcatcttt tcgattttgc
     3001 tatgacactc gatcataacg gggatgcatt tcattcagat ataaaaatta cgagtgaagg
     3061 tataactatt agtgacacgg aaggaagaga taaattttct atcagcgcaa gatggaatgt
     3121 tgaaggttac ggatatttat ttattccagc cgataatgag ggtgaatttt attcattacc
     3181 tgaatcgggc aaaattgaac taaatctgaa ctatgaattt gcaaaaacca gagtcagccg
     3241 aaatcgcaga agaaaaatta aatttgaaga aagcggatgg actccatcaa atgaactcag
     3301 acattatctt gaat
//
LOCUS       DASWBY010000750         5673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_368208, whole genome shotgun sequence.
ACCESSION   DASWBY010000750 DASWBY010000000
VERSION     DASWBY010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368208"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..429)
                     /locus_tag="VF870_13600"
     CDS             complement(94..429)
                     /locus_tag="VF870_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253275.1"
                     /translation="MKKLFTNYNFEFDKNEKKLLTNFCKQALKQVSGDSRFFAEEKAF
                     NSIIGKLNQGDDVVKFTKDEKTRLSHQLKQNSEFMKKEMKKSWFLKKWIYKSLFRQYD
                     SLIEKYFKD"
     gene            complement(431..1528)
                     /locus_tag="VF870_13605"
     CDS             complement(431..1528)
                     /locus_tag="VF870_13605"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX9253276.1"
                     /translation="MTTEEIIKTIPKVLLHDHLDGGLRAQTIIDLAQELNYDKLPTKN
                     PTELAEWFHRGANKGNLVEYLQGFEHTTAVMQTKDGLERIAYEMMEDMYKDGVVYVET
                     RFAPVYHRMKGLYYEDSVNAVLAGLERGKKDFGVGYGLILCGMRNMKDTVDIAELAVN
                     FRNEGVVGFDLAGEEGGYPPKKHIEAFQYIQRANFNITIHAGEAFGKDSIWQAIQWCG
                     AHRIGHATRLLEDIVLDSNGNVVKFGDLAQYVLDKRIPLEICLLSNLHTGAVDKLENH
                     PFGILYKEKFRITINTDDRLMSDTTMTKEFLTAIKYFNLNFEDFEKITINSMKSAFIP
                     YKERLHYIYNVIKPGYQKMRDKILSFNNVKG"
     gene            1707..2849
                     /locus_tag="VF870_13610"
     CDS             1707..2849
                     /locus_tag="VF870_13610"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9253277.1"
                     /translation="MKHSKVFVLAFLMLSMVFVGYQCGSTEITSAKLYIQQKNYDKAV
                     EVLQKEITKNPKSDEGYYLLGYVQGEQGNFDQMVDSYDKSLAISKNFEKNISDSRKYF
                     WAQAFNRGVSLYQRGVKTTDTDSAKVFYDKSIADFKSALKIEPDSADTYKNLAFVYLS
                     KGDNVAAVDPLKELIAREQSLDGYKFLGEIYYVNGTNLKAQGKNDEAKAEFNKSIEVL
                     EEGLKIYPDNAEMLVTLSTAYIGAERGKEAIEKYRKLVDAKPDDKNIRYNYGVLLLGA
                     DDFAGAETQFKKAIEIDPEYDNAIYNLGVTYLKWGTYLNKKADEEGKVSDEYKAKYQL
                     ALPYLEKAVQMKDANAQTWELLGKVYSVLGMTDDANNAFKKADEMR"
     gene            2858..3184
                     /locus_tag="VF870_13615"
     CDS             2858..3184
                     /locus_tag="VF870_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX9253278.1"
                     /translation="MDQNKQPQGQQLNIELGEKEAEGIYSNLAIISHSPAEFIIDFTR
                     IVPGVPKAKVYSRIITTPQHAKMLMKALIDNIDKFEAKFGEIKIDSAPNQMFGFVPPK
                     SNEKVN"
     gene            complement(3261..4343)
                     /locus_tag="VF870_13620"
     CDS             complement(3261..4343)
                     /locus_tag="VF870_13620"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX9253279.1"
                     /translation="MKIIDKYLIKQFLQTIAFGLLGFTFIFIVIDAMENLDDFIDQSV
                     PTLTIIHYYIVFSPEIIRLMTPVAVLFAALFTAGKAANLSELTAIKASGVSLFRFMLP
                     FIITTILISLFSIYFSGYVVPMANKTKLNIEQEYLKKNITFSGNNIYFQDSKKRIVSI
                     SFFDNNSNRANRVSIQDFNQNDLKQMQSRIDAVSLTYDTLKHVWIANNGIKRDFHGIH
                     QDAEYFKSMEIPNLNFSPADLLQKQTRPSEMNLSQLSDLAKSQQNAGNDPTSTLIEFH
                     SRIAFAMTSVIVVLFGLPISANKRKGGIASQVGLNILVTFLYLVFMKVSQAFGKNGAL
                     NPLLTAWLANFLFMIAAAVNLWRAKQ"
     gene            complement(4330..>5673)
                     /locus_tag="VF870_13625"
     CDS             complement(4330..>5673)
                     /locus_tag="VF870_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX9253280.1"
                     /translation="IPFVFSLITLMCIFLLQFLMKFADRLVGKGLDTWVITKLIAYNL
                     SWMVVLVVPMASLVATLMAFGNLSQNNEVTIMKSSGISLYKMIFPPFVASIVVAILLF
                     QFNNDVLPDANHQAKILMSDISRTKPTLSLEPGFFSQEVNNYAILVRSIDQKTNWLYQ
                     ITIYDYSNGAKINVVTADSGRIFFSKDQSKLLMDLYHGEIHESDVQNTTLYRKLEFEK
                     HIITMAGDQFSLQQSGPTSRGERELSVNDMQIITDSIKKVLNQNTILFTKEVNRYLFL
                     DTTTVRNTSKSNLVTNPELVYLRAIEKVKTVKNIVMSNLNRVEWSKRELEKYEVEIYK
                     KYALPGACIIFILIGAPLGVMVRKGGFGVAAGISLFFFLIYWAFLIGGEKLSERGFFS
                     PFIGMWAANIVLGIVGIILTIKTNKEAKTITFNFFKKLIPKKFRQKDIAAVDENN"
BASE COUNT         1881 a          972 c          944 g         1876 t
ORIGIN      
        1 tcaacatacg cattgcagct tcttgtatcc atcctttgca ggagatctcg gttccggttg
       61 gggcgatgat atttaaatgt tctgttaaca ttattaatcc ttaaaatact tttcaattaa
      121 actatcgtac tgtctaaata aagatttata aatccatttc tttaagaacc agcttttctt
      181 catttccttt ttcataaact cagagttttg ctttagctga tgtgataatc ttgttttttc
      241 atctttggta aatttaacaa catcatctcc ctggtttaat tttccgatta ttgaattgaa
      301 tgctttttct tcagcaaaga atctgctatc acctgatact tgtttaagag cttgcttaca
      361 aaaatttgtt aataattttt tctcgttttt atcgaattca aaattatagt tagtgaacag
      421 ttttttcatt ttatcctttt acattattaa acgatagtat tttatctctc attttttgat
      481 aacccggttt gattacattg taaatgtagt gcaatctttc tttgtacgga atgaatgcag
      541 atttcatgga gtttattgtg atcttttcaa aatcttcaaa gttcaggttg aaatatttaa
      601 tggcagttag aaactccttt gtcatagttg tatcactcat caacctgtcg tctgtgttta
      661 ttgtaatacg gaatttttct ttatacaaaa ttccgaatgg gtgattttct aatttgtcaa
      721 cagcacctgt atgaaggtta cttaataaac atatttcaag aggaattctt ttatccaaaa
      781 cgtactgagc aagatctcca aattttacaa cattaccatt tgaatcaaga acaatatctt
      841 ccagcaatcg tgttgcatga ccgattctgt gagcgccgca ccattgtatt gcctgccata
      901 tcgagtcttt tccgaaagct tcaccagcat gaattgtaat attaaaattt gcccgttgaa
      961 tgtattgaaa tgcttcgata tgttttttgg gaggatagcc gccttcttca cctgcaagat
     1021 caaatccaac aacaccttca tttctaaaat taaccgcaag ctctgctata tcaacagtgt
     1081 ctttcatatt tcgcataccg caaagtatta aaccataacc aactccaaaa tcttttttgc
     1141 ctctttctaa tccggcaagc acagcattta cagaatcttc gtaatacaat cccttcatgc
     1201 ggtgataaac aggtgcaaat cttgtttcaa cataaacaac tccgtcttta tacatgtctt
     1261 ccatcatctc atatgcaatc cgctccagcc catcttttgt ttgcataact gcggtagtgt
     1321 gctcaaatcc ctgaagatat tctaccagat tgcccttatt ggcgccgcgg tgaaaccatt
     1381 ccgccagttc ggtaggattt tttgtgggaa gtttatcgta attaagttct tgtgcaagat
     1441 caataattgt ctgtgctcta agcccgccgt caagatgatc atgaagaagt acttttggta
     1501 ttgttttgat gatttcttca gtagtcacaa taaacctctt ttttgttacc aaaaaatatc
     1561 cttttaagta tgtaaaatca atttaaatag cataatgaac tactaagaaa tggctatata
     1621 ttttgaaaaa tgtttgtatc tttgaaccat aaaattacaa ttctataaat aagggaatta
     1681 attttttact ttaaaacgga gaaaatatga aacattctaa agtgttcgtt cttgcatttc
     1741 tgatgctaag tatggttttt gttggatacc aatgcggttc cactgaaatt acaagtgcta
     1801 aattgtacat ccagcaaaaa aattatgata aagcggtaga agttttacaa aaagaaatta
     1861 ctaagaatcc taaaagcgac gaaggttatt atttgttagg atatgtacag ggagagcagg
     1921 gaaattttga tcagatggtt gacagttatg ataaatcact tgcaatcagc aaaaattttg
     1981 agaaaaacat cagcgactca agaaaatatt tttgggcaca ggcttttaac cgaggtgtaa
     2041 gcctttatca aagaggagta aaaaccactg atacagacag tgctaaagtc ttttatgata
     2101 aatcaatcgc tgattttaaa tcagcactaa aaattgaacc agattctgct gatacttata
     2161 aaaatcttgc atttgtgtac ctaagcaaag gtgacaatgt agctgctgtt gacccattaa
     2221 aagaattaat tgcccgtgaa caatctcttg atggatataa attcttaggt gaaatttatt
     2281 acgttaatgg aactaatctt aaagcacaag ggaagaacga tgaagctaaa gctgagttta
     2341 acaagagtat tgaagtttta gaggaaggtt taaaaatata tcctgataat gcagagatgt
     2401 tagtaacctt atcaactgct tatattggtg ctgaaagagg caaggaagct attgagaaat
     2461 acagaaaact agttgatgct aaacctgatg acaagaacat aagatataac tacggtgttc
     2521 tattgttagg tgcagacgat tttgctggcg cagaaactca atttaaaaaa gcaattgaaa
     2581 ttgatcctga atacgataat gcaatttaca acttaggtgt tacctatctt aagtggggaa
     2641 cttacttgaa taaaaaagct gatgaggaag gaaaagtttc tgatgaatat aaagctaaat
     2701 atcaattagc gttaccttac ttagaaaaag ctgttcaaat gaaagatgct aacgcacaaa
     2761 cctgggaact attaggaaaa gtttattcag tacttggtat gactgatgat gcaaacaatg
     2821 ctttcaaaaa agcagatgaa atgagataat tgaaattatg gatcaaaata aacaaccgca
     2881 gggacaacaa ttaaacatag agcttggtga aaaagaagct gaaggaattt attccaatct
     2941 tgctataatt tcgcattcac cggcagaatt tataattgat tttactagaa ttgttcccgg
     3001 tgtaccaaaa gcaaaagttt acagcagaat tattacaact ccgcagcacg caaaaatgtt
     3061 gatgaaagct ttgatagata atattgataa gtttgaagct aagtttggcg agattaaaat
     3121 tgattctgca ccaaaccaga tgtttggttt cgttccgcct aagagtaacg aaaaagtaaa
     3181 ctagatattc tgatcattta ttaaattaaa gccggttaat tttaaccggc ttttttgtat
     3241 ccgctttttt taagaaggaa ttattgtttt gcccgccaaa gattaacagc tgccgctatc
     3301 ataaacaaaa aattagctaa ccaggctgtt aacaaaggat ttaaagcccc gtttttccca
     3361 aaggcctgac ttactttcat aaaaaccaaa tacaaaaaag ttacaagaat gtttaatcca
     3421 acctgtgaag caatgccgcc ttttctctta tttgctgaaa tgggtaaacc aaatagaacc
     3481 actattacac tagtcatagc aaaagcaatt ctggaatgaa attcaattaa tgtagaagta
     3541 ggatcattac ccgcattttg ttgtgacttg gcaagatcac taagttgcga gagattcatt
     3601 tcagatggtc gggtttgttt ttgcagaaga tcagcaggag agaaatttaa atttggaatc
     3661 tccatcgatt taaagtactc tgcgtcctga tgtatgccat gaaaatctct tttaattccg
     3721 ttgtttgcaa tccaaacgtg ttttagtgta tcgtatgtta aactcacagc atcgattctg
     3781 gattgcattt gtttaagatc attttgatta aaatcctgaa tactgactct gtttgcgcga
     3841 ttagaattat tatcaaaaaa agaaatgctt acaatccttt tttttgaatc ctgaaagtaa
     3901 atgttgttac ccgaaaaagt aatattcttt ttaagatatt cctgttcaat gtttagcttc
     3961 gttttgtttg ccataggaac aacgtagccg ctaaaataaa tcgaaaataa actgattaga
     4021 atagtggtga taataaatgg aagcataaat ctgaaaagac taactccact tgctttaatt
     4081 gccgttaact cacttaaatt agctgctttg cctgctgtaa aaagagcagc aaataaaaca
     4141 gcaacgggag tcattaaacg aattatctca ggtgaaaaaa caatatagta atgaattatt
     4201 gttaatgttg gtacactttg atcaataaag tcatccaggt tttccatcgc atcaattaca
     4261 ataaaaataa atgtaaatcc gagtaaacca aacgcaatgg tttgcagaaa ctgtttgatt
     4321 aaatatttat caattatttt catctacagc ggctatatct ttttgtctga attttttagg
     4381 aataagtttc ttgaagaaat tgaacgtaat tgttttagct tctttattcg tctttatagt
     4441 cagaattatc ccgacaatgc ctaatacaat atttgcggcc cacattccaa taaacggcga
     4501 gaaaaatcca cgttcagata atttttctcc gccaattaaa aatgcccaat aaataagaaa
     4561 gaagaataaa ctaattccgg ctgcaactcc aaatccgcct ttgcgtacca tgactcctaa
     4621 gggggcacca ataagtataa aaattatgca agcacccggc agagcgtact ttttgtaaat
     4681 ttcaacctca tatttctcaa gttcgcgctt tgaccactca actctgttca agttagacat
     4741 tacaatattt tttactgttt ttactttttc aattgcacgc aaatacacca actcgggatt
     4801 tgtaactaaa ttacttttgg atgtatttct aacagtggta gtatccagaa ataagtatct
     4861 attaacttct ttagtaaata aaattgtgtt ctgatttaaa acttttttaa tactgtctgt
     4921 tataatctgc atatcattaa cacttagttc tctttccccg cgagatgttg gaccggattg
     4981 ctgaagtgag aactgatcgc ctgccattgt tataatgtgt ttttcaaatt caagttttct
     5041 atataaggtt gtattctgaa catccgattc atgaatctct ccatggtaca aatccataag
     5101 tagttttgat tgatcctttg aaaaaaatat tcttccacta tctgcggtaa ctacatttat
     5161 ctttgcgccg tttgaataat cataaattgt gatttgataa agccaatttg ttttttgatc
     5221 aatcgatctg actaaaattg cgtagttatt aacttcctgc gaaaagaaac ccggttcgag
     5281 tgaaagtgtt ggtttggttc gcgaaatatc actcattaat atttttgcct gatgatttgc
     5341 atcaggaagc acatcattat taaactggaa gagaagaatt gctacaacaa tgcttgcgac
     5401 aaaaggcgga aatatcattt tataaaggct tattccggat gatttcataa tcgtcacttc
     5461 attattttgg ctaagatttc caaatgccat caaagtagcg actaatgaag ccattggcac
     5521 aaccagaaca accatccatg aaagattgta agcgattagt tttgttatta cccaggtatc
     5581 caaacctttg ccaactaatc tgtctgcaaa tttcatcaaa aactgaagca gaaaaatgca
     5641 catcagagtg attaatgaaa atacgaaagg tat
//
LOCUS       DASWBY010000751         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_294073, whole genome shotgun sequence.
ACCESSION   DASWBY010000751 DASWBY010000000
VERSION     DASWBY010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294073"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VF870_13630"
     CDS             <1..1231
                     /locus_tag="VF870_13630"
                     /inference="COORDINATES: protein motif:HMM:NF025846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9253281.1"
                     /translation="SRLTAVDLIGLQSETGLTKQETIDSLSNLEFLGIIEFSKADGKE
                     SISLTTPRVRSEELKLNYKLINELYISSRQKLDRMVDFVYKNDCRFSYILKYFGEDAN
                     TFKCGKCDNCLKQNGVMENSTSYIKEKIEDLLYEELYELTEKQISDILLGKSKNTEHK
                     KSQHFNSLINYKREDISNAIRILVSENKIQQKSEGRNKIFFIINNNIEQPQISESVIA
                     PNKSFEDNIELYHSLRELREKASKKFLQSANIICPDAVLAKISQQKPKTKSELFSIPG
                     FNERMFNKVGNDFLELINSFETGKINTKEKVEIIIPQNILETYNLLQKKYSLQEIAKL
                     RKLNEAVISMQIETIIGYLPETDVSSIISKDKMDLIFETYRKGIKGLKELKETLPKEF
                     SYPQIRIALAKISRPSV"
     gene            complement(1167..1532)
                     /locus_tag="VF870_13635"
     CDS             complement(1167..1532)
                     /locus_tag="VF870_13635"
                     /inference="COORDINATES: protein motif:HMM:NF025494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HEX9253282.1"
                     /translation="MRSSLIKIALFIIVLFPGLQSCNKSSVIDEKKFIKIYADMIFMQ
                     DTSSLSQSTIKNKVLEKYNVKEIQYDETIKFYNDDPERWQKFFDSTVVYIESLKPKPK
                     QTDEKSLPEQSLSADKKIL"
     gene            complement(1529..>2200)
                     /locus_tag="VF870_13640"
     CDS             complement(1529..>2200)
                     /locus_tag="VF870_13640"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9253283.1"
                     /translation="GFWHIFFNMFILWMFGATIEDIMGSRKFLIFYLLAGVSAGLFQL
                     FITPLLGASEAVTIGASGSIFGVMIAYAMYFPDNLIYVYFLIPVKAKYFIGFLVIIEF
                     LAVDSASSGVAHLAHLGGALFGFLYIMFDKNSYVSLKNLFNRSFYYKSKPKHNIFTNP
                     FSSSSHNDSNVQDANYYELDNKEDPEVSQTEIDKILDKISQSGYQNLSEHEKKILFQA
                     SKKMK"
BASE COUNT          850 a          331 c          321 g          698 t
ORIGIN      
        1 ttcaagatta accgctgtag atttaatcgg tctacaatca gaaacgggat taacaaaaca
       61 agaaacaatt gattcattat caaatcttga atttcttggt atcattgagt tttcaaaagc
      121 ggatgggaaa gaatcaattt cattaacaac tcctcgtgtt cgatcagagg aattaaagct
      181 aaactacaaa ttgataaatg aactttatat cagttccagg caaaaattag ataggatggt
      241 tgattttgtc tacaaaaatg attgcagatt cagctatatt ctaaaatatt ttggtgagga
      301 tgcaaacact tttaagtgcg gtaaatgtga taattgtctt aagcaaaatg gcgtaatgga
      361 gaattctaca tcatacatta aagaaaagat tgaagatttg ctctacgaag aattatatga
      421 attaactgaa aaacaaattt cagatatttt actaggtaaa agtaaaaaca cagagcacaa
      481 aaaaagtcaa cattttaatt cattgattaa ttataaaaga gaagatatct cgaatgcaat
      541 aagaatttta gtaagtgaaa ataagattca gcaaaaatca gaaggcagaa acaaaatatt
      601 ttttataatt aacaataaca ttgaacaacc ccagatttca gaatcagtaa tcgcaccgaa
      661 taaaagtttt gaagataaca ttgaattgta ccattcactt agagagttaa gagaaaaggc
      721 ttctaaaaag tttttacaat ctgctaatat aatttgccct gatgcagttc tggctaaaat
      781 ttcacaacaa aaaccaaaaa caaaatctga gttatttagc attcccggtt ttaatgaacg
      841 tatgtttaat aaggttggaa atgattttct tgagttaata aattcctttg aaactggtaa
      901 aataaataca aaagaaaagg tagagatcat tattccccag aacattcttg aaacatataa
      961 tcttctacaa aaaaaatatt cccttcaaga aattgctaag cttaggaaac tcaatgaggc
     1021 tgttatatcc atgcagatag aaacaataat tggatattta ccagagactg acgtttcatc
     1081 aattatatct aaagataaaa tggatttaat ttttgaaact tatagaaaag gtataaaagg
     1141 gttaaaagaa ttaaaagaaa cgctgcctaa agaattttct tatccgcaga taaggattgc
     1201 tctggcaaag atttctcgtc cgtctgttta ggttttggtt ttaaactttc tatataaact
     1261 actgtactat caaaaaactt ttgccatctc tcaggatcat cattgtaaaa tttaatagtc
     1321 tcatcatatt ggatttcttt aacattatat ttttctaaaa ctttattctt aattgttgat
     1381 tgagataaag aagaagtatc ctgcataaaa atcatatctg catatatttt tataaatttt
     1441 ttctcatcaa taacagaaga cttattgcag gattgtaaac cgggaaatag aacaataata
     1501 aataaagcaa ttttgattaa actgcttctc atttcatttt cttgcttgct tgaaataata
     1561 ttttcttttc atgctcagaa agattttgat aaccggattg acttatttta tccagaattt
     1621 tatcaatctc tgtctgcgat acttcagggt cttctttatt atctaattca taataatttg
     1681 cgtcttgaac attgctatcg ttgtgagatg aactactaaa tggattagta aaaatattat
     1741 gtttaggttt tgacttatag tagaaggatc gattgaaaag attttttaat gatacataac
     1801 tattcttatc aaacataata tataggaatc caaataatgc tccgcctaaa tgtgctaagt
     1861 gcgcaacacc gcttgaggca ctgtcaacag caagaaattc tataataact aaaaatccga
     1921 taaaatattt tgccttaaca ggaattagaa agtacacata tattaaattg tctggaaaat
     1981 acattgcgta agctatcatc acaccaaaaa tggaacctga agcgccaatg gttacagctt
     2041 ctgaagcacc taataatgga gtaataaaca actgaaacaa tccggcactt acacccgcta
     2101 acagataaaa tatcaaaaac ttccttgacc ccataatatc ttcaatggtt gctccaaaca
     2161 tccataaaat aaacatatta aaaaatatgt gccaaaaacc
//
LOCUS       DASWBY010000752         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_399486, whole genome shotgun sequence.
ACCESSION   DASWBY010000752 DASWBY010000000
VERSION     DASWBY010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399486"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VF870_13645"
     CDS             complement(<1..400)
                     /locus_tag="VF870_13645"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX9253284.1"
                     /translation="MSFSASKGYFLKNGKPVFVVSGEIHYFRLDPKLWEKHLTLLKQS
                     GANTTSTYIPWDWHEYEEGKFDFKGNTNPARNLIKYIKLCKKVGLNLIVKPGPYILAE
                     YEHHGLPIWLLNKISKDAHALDEQGNVFDPD"
     gene            complement(420..>2378)
                     /locus_tag="VF870_13650"
     CDS             complement(420..>2378)
                     /locus_tag="VF870_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253285.1"
                     /translation="KVTWNCSDVNLEIKAFSDGEANISSTLYLKYRITNISKEQKEGK
                     LFLLIRPYQVNPHYQWLNNPGGVGKINSLNEKDGKIYVNDDKVLIPMEKCDSFCSITF
                     DEGNIVELIKENTILKNKSVLDDNKLASGVLEYSFNLKSGEEKCFYLAIPFYDEQSIK
                     DNLNEDLVEKKLNETINFWKDEVNHIKFNLPESADRIINTYKSNLAYILINRDKAGIQ
                     PGSRSYERSWIRDGSLTSSALLKSGIVNEVKDFIDWYASNQYEDGKVPCVVDKRGPDP
                     TPENDSHGELIYLIREYFNFTKDTTFLRSKNENVKKAVEYIESLIAERSTDHFKYGND
                     SVRAYYGLVTESISHEGYSAKPMHSYWDNFFTLKGLKDATEIQKVLGDKTSYERIKKV
                     KDTFKTNLYNSIKLAMKIRKINYIPGCVELGDFDPTSTTTALTPCNEFNNLPKPEVYN
                     TFDKYFKFFTDRRDNKINWINYTPYENRLIGSFISLDQPERAHQLIDYFLNDQVPHNW
                     NAFAEVVWKDKRIPKFIGDIPHTWVGSDFINAIRSMFVYENEYDSSLVLAAALYQDWI
                     DSPNGMAIENLPTYYGEISYSIKKENKKYIFNINGDVSLPENGIKVKNFNSSKLPVSV
                     NINGKETKSFTNNEIDVKEFPAVIEINY"
BASE COUNT          740 a          417 c          332 g          889 t
ORIGIN      
        1 aatccggatc aaatacattt ccctgttcat ccagggcatg tgcatcttta ctgattttat
       61 ttagtaacca gattggtaat ccgtgatgct catattcggc aagaatgtaa ggacctggtt
      121 ttacaattaa attcaatcca acttttttac agagcttgat atactttatt aaatttcttg
      181 caggatttgt gtttccctta aaatcaaatt taccttcttc atattcatgc caatcccagg
      241 gaatgtatgt tgaagttgta ttagctccgg attgtttaag aagcgttaaa tgtttctccc
      301 aaagttttgg atcaagcctg aaatagtgaa tctcacccga gactacaaaa acaggtttac
      361 cattttttaa aaagtatcct tttgatgcgc tgaaactcat ttaattcatt tctttagttt
      421 taataattaa tttcaattac tgcaggaaat tcttttacat ctatttcatt gttcgtaaaa
      481 gattttgttt ctttaccatt tatattaaca ctaacaggaa gtttagaact attaaagttt
      541 tttactttta ttccattttc aggaagacta acatcaccat tgatattaaa aatatacttt
      601 ttattttctt ttttaattga atacgaaatc tcaccgtagt aagtaggtag attttcaatt
      661 gccataccat tcggcgaatc tatccagtcc tgataaagtg cagcagcaag aacaagtgat
      721 gaatcatatt cattttcata aacaaacatt gaacgaattg catttataaa atctgatcca
      781 acccaggtat gtggaatatc tccaataaat tttggaattc ttttatcttt ccaaacaacc
      841 tctgcaaaag cattccagtt atgcggcacc tgatcattta gaaaataatc tatcaattga
      901 tgcgcacgct ccggctgatc taatgaaata aacgagccaa tcaatcggtt ttcgtatgga
      961 gtatagttta tccaattgat tttattatcg cgtctatccg taaaaaactt aaagtattta
     1021 tcaaatgtat tataaacttc tggcttaggc agattattaa attcattaca aggagtaagt
     1081 gcagtggttg tagatgttgg atcaaaatca cctagctcaa cacagccggg aatataattt
     1141 atcttcctta ttttcattgc aagctttatt gagttataaa gatttgtttt aaatgtatcc
     1201 ttcacttttt ttattctctc ataagatgtt ttatcaccaa gaactttttg aatctcagtt
     1261 gcatccttca aaccttttaa agtaaagaaa ttatcccagt aagagtgcat cggttttgca
     1321 gagtaacctt catggcttat tgattctgtt actaatccat aatatgctct aacactatca
     1381 ttcccatatt tgaagtgatc agttgatctt tcagctatta aagattcaat gtactcaaca
     1441 gcttttttta cattttcatt ttttgatctt aagaaagttg tgtctttggt aaaattaaaa
     1501 tactcgcgga tcaaataaat caactcgcca tgactatcat tttcaggagt cgggtccgga
     1561 cctcttttat caacgacgca tggtactttt ccatcttcat attgatttga tgcataccaa
     1621 tcaatgaaat cttttacttc attcacaatc cctgatttta ataatgctga tgaagttagt
     1681 gaaccatccc ttatccagct tctttcataa gaacgggagc cgggctgtat tccggcttta
     1741 tccctattaa ttaaaatata agccagattt gatttataag tatttattat tctatctgca
     1801 gattccggaa gattaaattt tatgtgattt acttcatctt tccagaaatt gattgtttcg
     1861 tttagtttct tttcaactaa atcttcatta agattatctt tgattgattg ttcatcataa
     1921 aaaggtattg cgagataaaa acatttttct tcacccgatt taaggttaaa actatattct
     1981 aaaactccac ttgcaagttt attatcatct aaaactgatt tgtttttaag aattgtattc
     2041 tcttttatta gctcaacaat attaccttca tcaaaagtaa tagagcaaaa tgaatcacat
     2101 ttttccattg gaattaaaac tttatcatca ttcacataaa ttttaccatc tttttcattt
     2161 aaagaattaa ttttaccaac tccacccgga ttgtttaacc attggtaatg cggattaacc
     2221 tgataagggc gaattaataa aaataacttg ccctcttttt gttctttgga gatatttgtg
     2281 attctgtact tgaggtaaag tgtagaacta atatttgctt cgccatctga aaaagcttta
     2341 atttcaagat ttacatcact gcaattccag gtgacttt
//
LOCUS       DASWBY010000753         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_31754, whole genome shotgun sequence.
ACCESSION   DASWBY010000753 DASWBY010000000
VERSION     DASWBY010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31754"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VF870_13655"
     CDS             complement(<1..620)
                     /locus_tag="VF870_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEX9253286.1"
                     /translation="MKTHIKQVIKRTGAIVPFNQERIANAIYRAAVAVGGRDKSTAQS
                     LSEQVVESLNKKFPEGTTPHIEDVQDIVEKVLIENGHAKVAKEYILYREEAAHRRDAE
                     GRYASKTNENIPWQKIWRNLDWAVSHNLHTVDHLNERIAQGEFPHIVHESETLYEDDV
                     ELAANLIIERLDALRMVMISGPSSSGKTTTTIKLEQKLIKKGFKFKA"
     gene            complement(1184..>2487)
                     /locus_tag="VF870_13660"
     CDS             complement(1184..>2487)
                     /locus_tag="VF870_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX9253287.1"
                     /translation="YYEAVWTVSFGDIDGDGEGEVVAGIGGPLGGHIQAFHKDGSVVT
                     GFPVNVGKYPMSATLADLDGNGTMEIILGTRTYKAYVYNGDGTVYPGWPKQMDRYVGA
                     SASAGDVNNDGIMDVIMESRNLLYVWNKDGQPLPGFPYVILDTLTGSNSYSAPVLADL
                     TGDGKQEIIFCSHSDATNAGGIIYAVKYDGTNIPGVPKTVPNWIYGAPIVADIDGDSQ
                     LEIVIGEYGSSPTPAFSVFAYNVDGSLVNNFPMGPYHGSANQITVADIDNDNEFEIIF
                     DENVTENGLGRYRALNMDGTDVTGWPLELNQNTSFQQPLLGDLNNDGILDLVGGSFNF
                     DIGNKQVFLYAWNTGLPYNPTKIVNPMYQFNPQHTGVFVDPSVIPVELESFMISLNGN
                     EVNLQWITASELNNSGFEVERSINNLNWNKIGFVNGQGTTA"
BASE COUNT          738 a          495 c          395 g          859 t
ORIGIN      
        1 gctttgaact taaatccttt ttttatcagt ttctgttcaa gcttaattgt tgttgttgtt
       61 tttcctgatg aagatggacc gctgatcata accattctaa gtgcatcaag tctttcaatt
      121 attaaatttg ctgcaagttc aacatcatct tcatataagg tttcagattc atgaacaata
      181 tgtggaaact ctccctgtgc aattcgctca tttaaatgat cgactgtgtg aagattatgt
      241 gaaacagccc aatcaagatt acgccagatt ttttgccatg gaatattttc attagtcttg
      301 ctggcatatc tgccttcagc gtcccttctg tgtgcggcct cttcacgata aagtatatat
      361 tcttttgcaa ctttagcatg tccgttttca atcaagacct tttcgacaat atcctgtaca
      421 tcttcaatat gcggggttgt gccttcagga aattttttat tcaatgactc aacaacctgc
      481 tcagataaac tttgtgcagt tgatttatct cttcctccaa ccgctacagc agcgcggtaa
      541 attgcatttg caattctttc ctgattaaaa ggtacaattg cgcccgttct tttaattacc
      601 tgtttaatat gtgttttcat tctttctcct gttgcaagaa atatttataa ctatccaaat
      661 ttatgaaata agatagtttg atacactttt catattaaga tttattattt tctttaaata
      721 aaaaacccgg tccaaaatga accgggttta attttgttaa aactctttta gaaccttatt
      781 tcatatatac cattttaatc atttgattag aaatttttcc tgttgttaaa gatgaagcat
      841 tgagtatcgc aaagtatatg cctgatgata agttagaggc atcaaataat tttaagtgat
      901 ttccttgctg ctgtacttca ttgaccaatt ctgttactac ttccccaaca acatttacaa
      961 tttttatttg aacgctgctt tcagaaggaa tgttatattt aattaaagta gaaggattaa
     1021 aaggattagg aaagttttgt tccagtttaa aatcacttat aagattataa tcaacataaa
     1081 ctatgttaga atatgtaaag gatccatcgt aatcaatctg ctttaatcta taatatagtt
     1141 tgccttcaag tgggttttta tcttcgaacg agtaaatagt tttttatgct gtggttcctt
     1201 gtccatttac aaacccaatt ttattccagt ttaaattgtt aattgatctt tcaacttcaa
     1261 atccggaatt atttaattcc gaagcggtta tccattgaag attaacttcg tttccattca
     1321 gcgaaatcat aaatgattcg agttcaacag gaattacgct gggatcaaca aaaacaccgg
     1381 tatgctgcgg gttaaattga tacatcgggt taactatttt tgtgggattg tagggaagtc
     1441 cggtattcca tgcatataaa aacacctgtt tattacctat atcaaaatta aaactgccgc
     1501 caactaggtc caggattccg tcattgttta gatcacctaa aagtggttgt tgaaaacttg
     1561 tgttttgatt taactctagc ggccagcccg taacatctgt tccatccata tttaatgctc
     1621 gatatctgcc caaaccattt tctgttacgt tttcatcaaa gattatttcg aattcattat
     1681 cattatcaat atctgcaacg gttatttgat ttgcagatcc gtggtatgga cccatcggga
     1741 aattatttac taaacttcca tcaacattat atgcaaaaac agaaaatgca ggagttggag
     1801 atgaaccata ttcgccaatt actatttcga gttgagaatc tccgtctata tcagcaacaa
     1861 tgggtgcccc ataaatccag ttgggaacag ttttgggaac cccgggaata tttgttccat
     1921 catattttac agcataaata attccgcctg catttgtagc atccgaatga gagcagaaaa
     1981 ttatttcttg ttttccatcg ccggttaaat ctgcaagaac gggggccgag taagaattag
     2041 atcctgtcaa agtatctaaa ataacatatg gaaatcctgg taaaggctgt ccatctttat
     2101 tccaaacata taaaagattt ctagactcca taataacatc cataattcca tcattgttta
     2161 catcgccggc agaagctgat gccccaacat atctatccat ctgttttggc catccgggat
     2221 aaacagttcc atcgccatta tatacataag ctttgtaggt tctggttcct aaaattattt
     2281 ccattgttcc atttccatct aaatctgcaa gcgttgcact catcggatat ttaccgacat
     2341 taaccggaaa accggtaaca actgatccat ctttatggaa tgcctggata tgtcctccga
     2401 gcggaccgcc aattcccgca actacttctc cctcgccatc accatcaata tccccaaagg
     2461 ataccgtcca tacagcttca taatatt
//
LOCUS       DASWBY010000754         3942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_67908, whole genome shotgun sequence.
ACCESSION   DASWBY010000754 DASWBY010000000
VERSION     DASWBY010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67908"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VF870_13665"
     CDS             <1..554
                     /locus_tag="VF870_13665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX9253288.1"
                     /translation="MFYHSTCGGQTEDVSNVFSKKNIAYLSGVKDGDQPFCKISPRYE
                     WSENYTESTFVDRLYAAQIIESKNYKISDIKINSKFSSGRVNELEIILSDSDGGEKNV
                     FMFGNRIRNIIRTGDSKSILKSTLFNITLDDKKNILITGKGNGHGVGFCQWGAIGQSK
                     MGIDYKEILNHYFPGTKIKSIYD"
     gene            547..1125
                     /locus_tag="VF870_13670"
     CDS             547..1125
                     /locus_tag="VF870_13670"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX9253289.1"
                     /translation="MISSSVPIFLASKSPRRRKLLKQLNLKFKSFSVDMDEKIHPDEQ
                     PFRAVVRLSKEKLALAKLKVKKGIIITADTIVVLDNKILGKPINKKDAFRILRFLSGR
                     THIVYTGYSIFNHSNGKTISEYEKTEVTFRDLTNQEIEDYINGGSPMDKAGAYGIQDD
                     FGAVFIKKINGCYYNVVGLPLAKFYHALLRIL"
     gene            1126..2091
                     /locus_tag="VF870_13675"
     CDS             1126..2091
                     /locus_tag="VF870_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX9253290.1"
                     /translation="MLQKIRNRVFLSIAVAALIYLTFMVYVDYEKVIKSFEKFNWYLL
                     PILLLLSLGNYISRFFKWQYYLNIIDVKLHKLDSLSVFMSGLIMSVTPGKMGELLKSY
                     LVKQVNGTSISKTAPIVLAERATDFLSLTILALAGAYFYDYGKNIIIIIGAIILAGLI
                     ILSNKKLFNKIISLISNIGFISRHTLKITTAYESSSKLLSITPLLLMTLLSIVSWGFE
                     CFGYYLILTNFEIKIDVLWAFFSYSFATIVGALSMLPGGLGVTEGSLTLMLVQKGLSE
                     HSAFAATFIVRAVTLWFAVLVGAISVLFYQKRFGKIVIESENVTV"
     gene            2379..2795
                     /locus_tag="VF870_13680"
     CDS             2379..2795
                     /locus_tag="VF870_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX9253291.1"
                     /translation="MSQDNNLAKGFLFGFLAGGALGAVVALLTAPKSGKELRADIKSK
                     SEEYLDEAEKYLTEAKDKARELINEGKKKSEKIIHDARSKSEDILKDAEKVFKDAKVK
                     TTDVYNTGKEKFETEAERIKSSVKAGVDAYKEAKNS"
     gene            2804..3190
                     /locus_tag="VF870_13685"
     CDS             2804..3190
                     /locus_tag="VF870_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253292.1"
                     /translation="MDTVQILTVVLIICASALCIALIYYINKIVKSVQSINKDLNELS
                     SNLRPLLLSSTELFETLNKFTSEAKDQLRISKSILNDFRDRADKLLSIEDKIRSGVED
                     TVMPLVKNMQAVGKGVETFWRNFKNK"
     gene            3232..>3942
                     /locus_tag="VF870_13690"
     CDS             3232..>3942
                     /locus_tag="VF870_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9253293.1"
                     /translation="MKEYAPESIRNIALIGHGSSGKTSLTEIMLFTAGETNRIGNVLE
                     GNTVSDYTSNEIEKQISISTSLMHIEWNNKKVNILDTPGYSDFIGDVKSAMKVCDTAV
                     MVLKSAEGVEVGSEVSGRFVDEFGLPSAIIINKVDNEHSTFEATLEKAKQRLTNGAIA
                     VTFPASEGILFNTVIDVLKMKAFTYGDAGSKNISEAEIPENLKSKAEEYRTQLIEKIA
                     ETNEELMNKYFEAGSLSDA"
BASE COUNT         1406 a          559 c          709 g         1268 t
ORIGIN      
        1 taatgtttta tcactcaact tgcggtggac aaactgaaga cgtatcaaat gtttttagca
       61 aaaagaatat tgcatattta agtggcgtaa aagatggtga tcaaccattt tgtaaaattt
      121 caccaagata tgaatggagc gaaaattata ccgaaagtac atttgtagat agactttacg
      181 ctgcacagat tattgagtca aagaattata aaatttcaga tataaaaatt aactctaaat
      241 ttagttcagg aagagtgaac gaacttgaaa taattctaag tgattctgat ggaggtgaaa
      301 agaatgtttt tatgtttggc aatcggatca gaaatataat tagaacaggt gatagcaaat
      361 cgattctgaa aagtacatta ttcaatatta ctcttgatga taaaaaaaat attcttatta
      421 ccggtaaggg taatggacac ggagtcggtt tctgtcaatg gggagcgatc ggacaatcaa
      481 aaatgggaat agattataaa gaaattttaa atcattactt tccgggaaca aaaattaaaa
      541 gtatctatga ttagttcttc tgtaccaatt tttctcgcat caaaatcacc gagaagaaga
      601 aaattattaa aacaattaaa cctaaaattc aaatcattta gtgttgatat ggatgagaaa
      661 attcatccag atgagcagcc atttagagca gttgtaagat tatctaaaga aaaactggct
      721 cttgctaaat tgaaagttaa aaaaggaatt ataattacag ccgatacaat tgttgttttg
      781 gataataaaa ttcttggcaa accgataaac aaaaaagatg cattcagaat tttaagattt
      841 ctaagcggaa ggactcatat tgtatacacc ggatactcaa tatttaatca tagtaacgga
      901 aaaacaattt ctgagtacga aaaaactgaa gttactttcc gagaccttac aaatcaggaa
      961 attgaagatt atataaatgg tggaagtcca atggataaag caggcgcata cggaatacag
     1021 gatgattttg gggctgtatt tataaaaaaa attaatggct gctattacaa cgttgtggga
     1081 ttaccgcttg caaaatttta tcacgcttta ttaaggatat tatagttgct gcaaaaaatt
     1141 cgtaatagag ttttcttatc aattgctgtt gccgcattaa tttatcttac atttatggta
     1201 tatgtcgatt atgaaaaagt aatcaaatca ttcgaaaaat ttaactggta tttgctgccg
     1261 attttgcttt tgctttcttt aggaaattac atatccagat tttttaagtg gcagtactat
     1321 ctaaacatta ttgatgtaaa acttcacaaa cttgattcgc tatcggtttt catgtctgga
     1381 ttaataatga gtgttactcc cggtaaaatg ggagaattat taaaatcata tttagtaaaa
     1441 caggttaatg gtacttcaat cagtaaaact gctccaatag tattagctga aagagcaact
     1501 gattttttat cattaacaat tttagcgctt gcgggtgcct atttctatga ttacggaaaa
     1561 aatataataa ttattatagg tgcgattatt ctggctggat taattattct atctaataaa
     1621 aaattattta acaaaatcat ttcgctcatt tcgaatatcg gttttatctc cagacatact
     1681 cttaagatca ccacagctta tgaatcttca tcaaaattac tgtcaataac tccattactt
     1741 cttatgacat tgcttagcat agtatcctgg gggtttgaat gttttggtta ttatctaatc
     1801 ttaacaaatt ttgaaataaa aattgatgtt ctctgggcat ttttcagcta tagttttgca
     1861 accatagttg gggcactttc tatgctgccg ggaggattag gagttaccga aggttcttta
     1921 acgctaatgc ttgtgcaaaa aggattatcc gagcacagtg cttttgcagc aacttttatt
     1981 gttcgcgcag ttacattatg gtttgccgtg ttagtaggtg ctataagtgt attgttttat
     2041 cagaagagat ttggcaaaat tgttattgaa tccgaaaatg ttacggttta ggattaatga
     2101 ataaaatact aattctcaaa gatcccgtag ggcaattcta atttttaatt gagcttaata
     2161 attaatacaa tccataagct tgaagtagat aaatagcatc aaatctttgg aatttgcacc
     2221 ataaatagat aaaaatcttt gattcgacat ttaaagctga ttgttaatta atgtcacact
     2281 aatcttaact aacttactcc aatttactct tttccttgat tttagaacgt tcaattgata
     2341 tattttgatc agtaatttat aaacgaaagg agtttgacat gtcacaagat aataatctag
     2401 ccaaaggttt cttattcggt tttttagccg gaggtgcgct tggtgctgtt gttgctttgt
     2461 taacggctcc taaaagcggt aaggagttac gagcagatat taaatctaaa tctgaagaat
     2521 atcttgatga agctgaaaaa tacttaactg aagctaagga taaagctcgt gaattgatta
     2581 atgagggcaa aaaaaaatct gagaaaataa ttcacgatgc aagatcaaaa tcagaagata
     2641 ttttaaagga tgctgaaaaa gttttcaaag atgcaaaagt aaaaacgaca gatgtttaca
     2701 ataccggtaa agaaaaattt gaaacagaag ctgaaagaat taaatcttct gttaaggccg
     2761 gtgttgatgc atataaagaa gctaaaaact cttagacact taaatggata cagttcaaat
     2821 tcttacagta gtattaatta tttgtgcatc tgcactttgt attgcactga tttattacat
     2881 aaataaaatt gttaaatcag tgcaatccat taataaggat ctgaatgaat tatcatccaa
     2941 ccttagaccg cttctactct cttctactga actttttgaa acccttaata aatttacttc
     3001 tgaggccaaa gatcaactaa gaatttcaaa gtccatttta aatgatttta gagatcgagc
     3061 tgataagtta ttgagcatag aagataaaat tcgtagcggt gttgaagata ctgtgatgcc
     3121 attagttaaa aatatgcaag ctgtcggcaa aggagttgaa acattttgga gaaacttcaa
     3181 aaataagtaa tcgtttaatt ctcaccttaa aaaattttag gaggagttcc cttgaaagag
     3241 tacgcacctg aatcaatacg taatatagct ctaattggac atggcagtag cggtaaaact
     3301 tcacttacag aaattatgtt atttactgca ggtgaaacaa acaggattgg taatgttctt
     3361 gaaggcaata cagtttcgga ttacacatca aatgaaatag aaaaacaaat ctcaatttcc
     3421 acttctctta tgcatatcga atggaataat aaaaaggtaa acatattaga tacacccggt
     3481 tactcagatt ttattggtga tgtgaaatca gcaatgaaag tttgtgatac tgccgtaatg
     3541 gttttaaagt ctgctgaagg tgtagaggtc ggttctgaag ttagcggaag atttgttgat
     3601 gaatttggac ttccatctgc gattataatt aataaagttg ataatgagca ttccactttt
     3661 gaagcaacgt tggaaaaagc aaaacaaaga cttacaaatg gtgcaattgc agttactttt
     3721 cctgcatctg aaggaattct ctttaatacc gtaattgatg ttctaaagat gaaagcgttt
     3781 acttatggtg atgcaggatc aaagaatatt tcagaagcag aaattccaga aaacctaaaa
     3841 tctaaagcag aagaatatcg tactcagttg attgagaaga ttgccgaaac caacgaagag
     3901 ttgatgaata aatattttga agccggctct ttgagcgatg ct
//
LOCUS       DASWBY010000755         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_745557, whole genome shotgun sequence.
ACCESSION   DASWBY010000755 DASWBY010000000
VERSION     DASWBY010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745557"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VF870_13695"
     CDS             <1..301
                     /locus_tag="VF870_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9253294.1"
                     /translation="KRFEKLHQDYPEIAKAYEQLGTAVHNSGKLDDKTRALIKLAIST
                     GAGLEGAVHSHTRKALELGISKEEIRQTVLLALPTIGLPSTMASMSWVDDILDKQ"
     gene            complement(352..1611)
                     /locus_tag="VF870_13700"
     CDS             complement(352..1611)
                     /locus_tag="VF870_13700"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX9253295.1"
                     /translation="MKKVFLFLFIIIGFTIHSTFATDGYYRHGYGIKYSALAGSGVAV
                     SLSSLGAISNPAGITQLNSGFEINVSYFSPDRYYEVGGNPSGFPGTFGLMPGKVVSEQ
                     TSFYFPTLGGNLKLSDKMAIALSIYGNGGMNTDYPTMTFGDPTSPSTGVNIEQMFVNL
                     SFAYKFDSNHSIGIAGIFGWQRFAAKGLIAFSNFSSDPANLTGNAWSTATGFGFKVGY
                     QGQWLKDLSFGAAYQSKIYMSEFERYAGLFAQAGDFDVPANWQAGLAFTPGDWKFLVD
                     VKQIFYSDVKSIANPMLPNLQTALLGQEEGAGFGWKDIMVIKYGIMYSGFCGWDLMAG
                     YSIGQNPIDADNVMFNILAPGVIQNQITFGFSKDIAVNHEISVAFMYAFENTVKGPNP
                     LDAPNQQTIEIGMKQWQLEVGYAFSSF"
     gene            complement(1762..>2174)
                     /locus_tag="VF870_13705"
     CDS             complement(1762..>2174)
                     /locus_tag="VF870_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9253296.1"
                     /translation="FNEYSIPNAQCIPLPQISSSELLRNQKIVLYSDDDIAASQAWFI
                     LKSKNYKGVYILDGGLNAWKEKVLFPKAPANAGKEELAEFEKMKEVAKYFGGQAQTDS
                     SVAETKQEIKIATPTTSTTKTSTPIGKKKKREGC"
BASE COUNT          692 a          428 c          365 g          689 t
ORIGIN      
        1 aaaacgattt gaaaaacttc atcaggatta ccctgagatt gcaaaagctt atgaacaact
       61 aggaacggcc gttcataatt cgggtaagct tgatgataaa accagagcac ttataaaact
      121 tgcaatatca acaggagcag gattagaagg cgcagttcat tctcacactc gtaaagcact
      181 ggaacttgga atttctaaag aagaaatcag gcaaacagtt ttgcttgctc taccaactat
      241 tggattacca tcaaccatgg catctatgag ctgggttgat gatatacttg ataagcaata
      301 aaaaaagggc atctaaatga tgcccttctc taccctgttc aaatttaaag attagaaact
      361 gctgaaagca taaccaactt caagctgcca ttgtttcatc ccgatttcaa tggtttgctg
      421 atttggtgca tcaagaggat ttggtccttt taccgtattt tcgaatgcat acatgaatgc
      481 aactgaaatt tcatgattta cagcaatgtc ttttgagaat ccaaatgtta tttgattttg
      541 aatgacgccc ggtgcaagaa tattgaacat aacattatca gcatcaatag gattttggcc
      601 aatagagtaa ccagccatta aatcccatcc acagaatcca gaatacataa tgccgtattt
      661 aattaccata atatctttcc atccaaaacc tgcaccttcc tcctgtccca ataaagcagt
      721 ctgcaaattt ggaagcattg ggttcgcaat cgattttaca tcactgtaaa aaatttgctt
      781 tacatctact aaaaatttcc aatctcccgg tgtaaaggca aggccagctt gccagtttgc
      841 aggaacatca aaatcaccag cctgagcaaa tagtcctgca tatctttcaa attcactcat
      901 ataaatcttt gattggtagg cagcaccaaa acttagatct tttaaccatt ggccttgata
      961 accgaccttg aatccaaaac ctgtagcagt tgaccatgca ttacctgtta agtttgctgg
     1021 atcactagag aagttactga atgcaattag tccttttgca gcaaatcttt gccaaccaaa
     1081 tattcctgca atacctatcg aatgattgga atcaaactta tacgcaaaag aaagattgac
     1141 aaacatttgt tcaatgttta caccagtcga tggagaggta ggatcaccaa aagtcatagt
     1201 tggataatca gtattcatac cgccattacc ataaatagaa agagcaattg ccattttatc
     1261 agagagttta agatttccac caagtgttgg aaaatagaat gaagtctgtt cactcacaac
     1321 ttttcccggc atcaatccaa atgttcccgg aaaacctgat ggatttccac caacttcata
     1381 atatctgtca ggactgaaat aagaaacgtt aatttcaaat ccgctgttca attgtgtaat
     1441 gcctgcagga tttgaaattg ctccaagtga actcaacgaa actgcaacac ctgatcctgc
     1501 aagtgctgag tattttatac cataaccgtg gcgataataa ccgtctgttg caaaagttga
     1561 atggattgtg aatccaataa ttataaatag aaacaagaaa acttttttca ttttatctcc
     1621 tctgacaaat aatattgtaa aataaatttt ttcgcactga gaaaattcga actgcgatct
     1681 tttagccatt ttctttaaag ttacatctct cgtattcgct caagatgata ttttaatttt
     1741 ttatttgtaa ttttttatgg atcagcaccc ttcccttttt ttcttttttc ctatcggggt
     1801 tgaagttttt gttgtgcttg tagtcggtgt cgcaattttt atttcctgtt ttgtctctgc
     1861 aacagaagaa tcagtttgtg cctgacctcc aaaatatttt gcaacttctt tcattttttc
     1921 aaattcggct aactcttcct tgccagcatt ggcaggagct tttgggaaaa gaactttttc
     1981 tttccaggca ttcaagccgc catcaaggat ataaacacct ttgtagtttt ttgattttag
     2041 aataaaccaa gcttgagatg cggcaatatc atcatccgag tataaaacta ttttttgatt
     2101 tcttaataat tcagatgatg aaatttgtgg aagtggaata cattgagcat tgggaattga
     2161 gtactcatta aatt
//
LOCUS       DASWBY010000756         3488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_328814, whole genome shotgun sequence.
ACCESSION   DASWBY010000756 DASWBY010000000
VERSION     DASWBY010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328814"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /locus_tag="VF870_13710"
     CDS             complement(<1..1586)
                     /locus_tag="VF870_13710"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX9253297.1"
                     /translation="MEKSVIKEFLKHIQLFKGLNDSQLEAICDKVKVENFPVNSMVFN
                     ENNIRQNLFLIYEGEVELFKRTPYGGEKRLSIFSKYDFIGEGALMDDSPHSTSARATI
                     NSVIVTLSRASFKELMKEQNETALAILSSIGRVISRRMSAANTRVINVAAQYQSGRTR
                     SEHDLLGDREVPHEYYYGVQTLRALENFNISGVTLNFYPVLIEALAMVKKAAAKANYD
                     LGLLSKPITDAIMQACDEIINGKLHSHFVVDMIQGGAGTSTNMNANEVIANRALEILG
                     YEKGEYKYCHPNNHVNLSQSTNDAYPTSVKIALINSNKKLTEVLQDLVQSFRNKAKEF
                     SQIIKMGRTQLQDAVPMTLGQEFEAYAVMLNEEVQRLDENVKLFLEVNMGATAIGTGI
                     NSHPDYSEKVIKHLKEVTGLNTVLADNLVEATQDTGAFIMYSSAVKRLAVKLSKICND
                     LRLLSSGPRTGIHEINLPPMQPGSSIMPGKVNPVIPEVVNQIAFKVIGNDLAVTMAAE
                     GGQLELNVFEPVIVQSLFES"
     gene            1855..>3488
                     /gene="leuS"
                     /locus_tag="VF870_13715"
     CDS             1855..>3488
                     /gene="leuS"
                     /locus_tag="VF870_13715"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9253298.1"
                     /translation="MRYPFTEIESKWQKFWEENKTYKTDLSKTDNKLYTLVMFIYPSG
                     AKLHIGHWYNYGPTDSWARFKKLQGYNVFEPIGYDAFGLPAEGYAIKTGIHPQDSTLK
                     NIEDIREQLKKMGCMYDWDAELMTCAPEYYKWNQWIFLQMYKRGLAYRKNAPVNWCPK
                     DQTVLANEQVIDGCCERCGTTVIQRNLYQWFFKITDYAEQLLDGHNKIDWPHETILKQ
                     QNWIGKSEGAEVTFKVANDVISKESSTEKYSTDTAKKISPSGRNDNTEELEIKVFTTR
                     PDTLFGVTYVVLAPEHPLVDKITKPEFKAGVDDYRESTKKLTEIERTSTVKEKTGVPT
                     GAYAINPVNGEKIPIWIADYALITYGTGCVMAVPAHDERDFEFATKFNLPIRKVILQD
                     GTNENDESKSAFTEIGTMINSGMFNGVRSDEGITKVIKYLEENNFGTGKINYRLRDWL
                     ISRQRYWGTPIPIIHCPACGEVPVDENELPVVLPYDVDFKLGGESPLARNEKYINVKC
                     SKCGVDAKRDPDTMDTFVDSSWYYLRFLDPKISTAA"
BASE COUNT         1097 a          641 c          646 g         1104 t
ORIGIN      
        1 atcgattcaa ataaactttg cacaattaca ggttcaaaaa cattaagttc aagctgaccg
       61 ccttctgcag ccattgtaac agcaagatca ttaccaataa ctttaaacgc aatttggttt
      121 acgacttccg gaataaccgg atttactttt cccggcatta tagaagaacc gggctgcatc
      181 ggaggaagat taatttcgtg aattcctgtt cgtggtccag atgaaagtaa acgcaaatca
      241 ttacaaattt ttgaaagttt aactgccagt ctttttactg ctgaagaata catgataaaa
      301 gcacctgtat cctgtgtagc ttcaacaaga ttgtcggcca gaacggtatt taatcccgtc
      361 acttctttta aatgcttaat tactttttcg gaataatccg gatgagaatt aattcccgtt
      421 ccaattgcag tcgcacccat attaacttca aggaacagct taacattttc atcaagccgc
      481 tgaacttctt cattcagcat tacagcataa gcttcaaact cctgcccaag agtcattgga
      541 actgcatctt gtagttgtgt tcttcccatt ttaatgatct gagaaaattc tttcgcttta
      601 tttctgaatg attgaacaag atcttgaagc acttctgtta attttttgtt tgaatttata
      661 agggcaatct taacagatgt cgggtaagca tcgttggtgg attgtgaaag gttaacatga
      721 ttgttaggat gacagtattt gtattctcct ttttcatacc ccagaatttc aagcgcacga
      781 tttgcaatca cttcgtttgc attcatattt gttgatgttc ctgctccgcc ctgaatcata
      841 tccacaacaa aatgtgagtg aagtttccca tttataattt catcacacgc ctgcataatt
      901 gcatcagtaa ttggtttaga taaaagtcca agatcataat tagcttttgc cgcagctttt
      961 ttaaccattg caagtgcttc gataagaaca ggataaaaat ttagagtaac tccgcttatg
     1021 ttaaagtttt caagcgcacg taaagtctgc acaccataat aatattcgtg cggcacttct
     1081 ctatcgccaa gtaaatcatg ttcgcttcgg gttctgccgg attgatattg cgccgcaaca
     1141 tttataactc gagtgttagc ggcactcatt cttcttgaga taactcttcc gatacttgaa
     1201 aggattgcaa gtgcagtttc gttttgttcc ttcattaact ctttaaagct agcacgtgaa
     1261 agtgttacaa taacagaatt gattgttgca cgagctgaag ttgaatgcgg tgaatcatcc
     1321 attaatgcgc cttcaccgat aaaatcatat ttgctaaaaa ttgataatct tttttctcca
     1381 ccataaggag ttcgtttaaa cagctccact tcgccttcgt aaattaaaaa aagattttga
     1441 cgaatattgt tttcattaaa caccatacta tttacaggaa aattttcaac tttaactttg
     1501 tcgcagatag cttctagttg tgaatcattt agtcctttaa aaagctgaat atgttttaag
     1561 aattctttta taaccgattt ttccatgtct gtaccttatt taataaaatt gacttttcaa
     1621 atttacttat aaatccaaca agcgaataaa aattttagtc gtaacgaaag ataactacca
     1681 gagtaagtcg gtaacatttg tacttgcctg caggtaggca agcttcgctc agtctgacat
     1741 taggttactc aataaactga ccatttttgc gattttatca atcaaacctc aatttgaatt
     1801 cccttctcaa atagctaaat ttggcttcaa ttttcaacaa ttatgatata aaaaatgcgt
     1861 tatcctttta cagaaataga atccaaatgg cagaagtttt gggaagagaa taaaacttac
     1921 aaaactgatc tttccaaaac ggacaacaaa ctttatacac tcgtaatgtt tatttatcct
     1981 tcaggtgcaa agcttcatat cgggcattgg tataattacg gaccaacaga tagctgggca
     2041 cgttttaaaa agcttcaggg ttataatgtt tttgaaccga ttggttatga tgcttttggt
     2101 ttgccggcag aaggttatgc aattaagact ggaattcatc cgcaggacag cacattaaaa
     2161 aatattgaag acattcgtga acagcttaag aaaatgggct gtatgtatga ttgggatgcc
     2221 gagttaatga cttgcgcacc tgagtattat aaatggaatc agtggatttt tcttcagatg
     2281 tataaacgcg gattagcata caggaaaaat gctcccgtaa actggtgccc caaagatcaa
     2341 actgtgcttg caaatgagca ggttattgat ggctgctgcg aacgatgcgg aacaaccgtt
     2401 attcaaagaa atttatatca atggtttttt aagattactg attacgcaga acagcttctt
     2461 gatggacaca ataaaattga ttggccacat gaaacaattt taaaacaaca aaactggatt
     2521 gggaaaagtg aaggcgctga ggttactttt aaggtagcta acgatgtcat ttcaaaggag
     2581 tcctcgactg agaaatattc tactgatacc gcaaaaaaga tttctccctc cggtcgaaat
     2641 gacaacactg aagaattgga aataaaagtt tttacaacga ggcctgatac cttattcgga
     2701 gttacttacg ttgtgcttgc accggaacac cctcttgttg ataaaataac caaaccggag
     2761 tttaaagctg gtgttgatga ttaccgtgaa tcgacaaaaa aactaaccga gattgaacga
     2821 acatcaactg taaaggaaaa aaccggagtc ccgacagggg cttatgcaat aaatcctgtt
     2881 aatggtgaaa aaattcctat ctggattgct gattatgcat taattaccta cggaacaggt
     2941 tgcgttatgg ctgttcctgc tcacgatgag agagattttg agtttgcaac taagtttaat
     3001 cttccaatcc gaaaagtaat tttgcaggat ggaacaaatg aaaacgatga atcaaaatct
     3061 gcttttactg aaattggcac aatgatcaac tcaggtatgt ttaatggtgt tcgctctgat
     3121 gaaggaatta caaaagttat aaagtatctt gaagaaaata atttcggtac tggaaaaata
     3181 aattatcgtt tgcgtgattg gctgatttcc cgccagcgtt attggggaac accaattccg
     3241 ataattcatt gtcctgcttg cggcgaagtt cctgttgatg aaaatgaatt accggttgtt
     3301 cttccttatg atgttgattt taaacttggt ggtgaatctc cgcttgcaag aaatgagaag
     3361 tatataaacg taaaatgttc aaagtgcgga gttgatgcaa aacgtgatcc cgatacaatg
     3421 gatacatttg ttgattcttc ctggtattac ttacgttttc ttgatccaaa gatttctaca
     3481 gcagcatt
//
LOCUS       DASWBY010000757         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_711444, whole genome shotgun sequence.
ACCESSION   DASWBY010000757 DASWBY010000000
VERSION     DASWBY010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711444"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VF870_13720"
     CDS             complement(<1..530)
                     /locus_tag="VF870_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEX9253299.1"
                     /translation="MAKSSNGAVEKVLKFIKDNGIKFVDLKFMDFPGQWQHFTVPVTH
                     FDGGSFEDGFGFDGSSIRGWKSIHESDMLLIPDAETMFVDLFVEAPTISLICDVYEPA
                     TKEKYSRCPRNIAQKAEAYLLSTGIADTVYYGPEAEFFVFDDVRFDSQPNGSFYIVDS
                     VEGKWNSGREENPNLG"
     gene            complement(618..1064)
                     /locus_tag="VF870_13725"
     CDS             complement(618..1064)
                     /locus_tag="VF870_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9253300.1"
                     /translation="MLDETDLQILRTLQKRGRTKRNELAEEVKLSIPSVSERLNKLED
                     KKIIEGYYAKLNRKAFNYDIMAFILVMMDSSKHYKNLITNVEKMPEILECHAVLGEGS
                     HLLKALVKNTEALEKVLSVIQSWQGVMGTKTTYVLSTVKETFELNV"
     gene            complement(1197..2006)
                     /locus_tag="VF870_13730"
     CDS             complement(1197..2006)
                     /locus_tag="VF870_13730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9253301.1"
                     /translation="MVKTSVIAAFYNNIKYLKLVLAGFERQTEKNFELIIADDGSTEE
                     VVKEIESIASNYPFRIKHIWHPDKGFRKNKILNQAILASESDYLIFIDSDCVPHSKFV
                     EEHLNEKEVQKVLTGRRVNLSQKITNELTEQNVKDEFIEKNYLKIIDDGLFGKSYDVE
                     KGFYFENKFLKKFFNKKYRGILGCNFSLYKKDLLDINGFDERYEAPSIGEDSDVEFRL
                     GLNGLKVKSLNHIAVQYHLYHKLQERLQKNLDLFEDVKKARIAYTPFGLKK"
     gene            complement(1999..2160)
                     /locus_tag="VF870_13735"
     CDS             complement(1999..2160)
                     /locus_tag="VF870_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253302.1"
                     /translation="MNKKTKIIVKKKKKRVPVPQKPPKVEKNPKAYDRDDEKKKIKKK
                     IQEDLNSNG"
     gene            complement(2220..2378)
                     /locus_tag="VF870_13740"
     CDS             complement(2220..2378)
                     /locus_tag="VF870_13740"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEX9253303.1"
                     /translation="MSKLSILSELWEFLKVRKKWWLLPIVLFLVLLGGLIIITQGSAL
                     APFIYAIF"
BASE COUNT          722 a          425 c          357 g          891 t
ORIGIN      
        1 taaccgaggt ttggattttc ttcccttccg ctgttccatt taccttcaac ggaatcaaca
       61 atataaaaac taccgtttgg ctgtgaatca aatcttacat catcaaaaac aaaaaactca
      121 gcttctggtc cgtaataaac tgtatctgca attcctgttg agagcagata tgcttcagct
      181 ttttgtgcga tgttacgcgg acagcgggaa tatttttctt ttgttgccgg ttcatacaca
      241 tcacaaatca aactaatcgt tggtgcttca acaaaaagat caacaaacat tgtttcggca
      301 tcaggaatta aaagcatatc gctttcgtgt atcgatttcc agcctctaat tgatgaacca
      361 tcaaaaccga atccgtcctc aaatgatcct ccatcaaaat gtgtaacggg aaccgtaaaa
      421 tgctgccact gcccgggaaa atccatgaat tttaaatcca caaattttat cccgttatct
      481 tttataaact tgagcacctt ttcaactgct ccattagaag attttgccat atagtttcct
      541 ttatgattta taaattatta gttaagaatt tcattagtca tactaagcgt agtcgaagta
      601 tgaaatttaa atattaatca aacattaagt tcaaaagtct cttttactgt tgatagtacg
      661 taagtggttt ttgtccccat taccccctgc caggattgga tcactgaaag aactttttcg
      721 agtgcttctg tattttttac taaagctttt agaagatgtg aaccttctcc tagtacggca
      781 tggcactcaa gaatttccgg catcttttca acatttgtaa ttagattttt ataatgtttt
      841 gatgaatcca tcattacaag aatgaatgcc attatatcat aattaaaagc tttacggttt
      901 agttttgcgt agtagccttc aattattttt ttatcctcaa gcttatttag tctttcgctt
      961 actgaaggta tggacagctt aacttcttct gcgagttcat tgcgttttgt tctgcccctt
     1021 ttttgtaaag tgcgtaaaat ttgtaaatct gtttcgtcca gcataaaaac cttaataatt
     1081 taggaacatt tgaattattg gtttcgaata taaggcgtag caaaaaatat tccaaactta
     1141 ttttagtatt ttttaaagaa tttactaaaa taattgcgcc agaatgagat tttgtattat
     1201 tttttcaggc cgaaaggggt gtaagctata cgtgctttct ttacgtcttc aaataaatcc
     1261 agattctttt gcagtctttc ctgaagttta tgataaagat gatactgaac agcaatatga
     1321 tttaatgatt ttacttttaa tccatttaaa cctaaacgga attctacatc agaatcttct
     1381 ccaatagatg gcgcttcgta tctttcatca aatccattta tatcaagtaa atccttttta
     1441 taaagagaaa agttacaacc aagaattcca cggtattttt tattaaagaa tttttttaga
     1501 aatttattct caaagtaaaa gcctttttca acatcataag atttaccgaa taaaccatca
     1561 tcaattattt taagataatt tttctcaata aattcatctt taacattttg ttcagttagt
     1621 tcattagtaa ttttttgcga aaggtttact cttctgcctg ttagaacttt ttgaacttct
     1681 ttttcattta aatgttcttc aacaaatttg ctgtggggaa cacaatcaga atcaataaat
     1741 atcaaataat cagattcaga tgcaagaatt gcctgattaa gaattttatt tttacgaaat
     1801 cctttatcag gatgccagat atgtttaatc cggaaaggat aatttgaagc gatggattct
     1861 atctctttta ccacttcttc tgtggagccg tcatcagcga taattaattc aaaatttttc
     1921 tctgtttgtc tttcaaatcc tgcaagaaca agttttaaat attttatgtt attataaaat
     1981 gctgcaataa cagaagtctt aaccattcga gtttaagtct tcctgtattt ttttcttgat
     2041 ctttttcttt tcgtcatctc tgtcataggc tttaggattt ttctcaacct ttggcggttt
     2101 ttgaggaaca ggaacgcgtt tctttttctt ttttacaatt attttggttt tcttattcat
     2161 aattttttgt agcgcagacc tgcctgcggt aagctggctt aagtctgcgt tacgttgttt
     2221 tagaatattg cataaataaa gggagccaat gctgaaccct gtgtaattat tatcagtccg
     2281 ccaagaagga ctaaaaataa aacgattggc aaaagccacc atttttttct taccttaaga
     2341 aattcccaca gctctgatag tattgataat ttgctcataa ttaatccgtt ttttt
//
LOCUS       DASWBY010000758         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_595281, whole genome shotgun sequence.
ACCESSION   DASWBY010000758 DASWBY010000000
VERSION     DASWBY010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595281"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VF870_13745"
     CDS             <1..331
                     /locus_tag="VF870_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX9253304.1"
                     /translation="EGKIDLGEVTDINKISIGFFQASSSWVVFPKYVEFFTSSDGIEY
                     QSAGKIDIPSSLRNPDWVQKDFFLDFKEVKGRYVKIFAKNLPELPPWHPEAGGKPWLM
                     IDEIIVE"
     gene            435..1085
                     /locus_tag="VF870_13750"
     CDS             435..1085
                     /locus_tag="VF870_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX9253305.1"
                     /translation="MKTAIVIGATGLVGSFITHKLLEDNRYTKVKVFVRRSLQINNPK
                     LEEHIIDFDKIELWKDRLTGDELYSALGTTIKKAGSKDAQFKIDFTYQYEAAKAGLAN
                     GVKTYLLVSSAGANSKSGNFYLRMKGNLDEKVQQLNFDKIYIFRPSILAGDRNEKRFG
                     ESIGIKIAGTITKIIPALKKYRPIEASKVAEAMIKSATQNLVDKIRIYQPEEIFNI"
     gene            complement(1088..2902)
                     /gene="typA"
                     /locus_tag="VF870_13755"
     CDS             complement(1088..2902)
                     /gene="typA"
                     /locus_tag="VF870_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX9253306.1"
                     /translation="MKKIRNIAIIAHVDHGKTTLVDQILKQCAVFRTNQVVRERFLDS
                     NDLERERGITILAKNISIPYKDYKINVIDTPGHADFGGEVERVLKMADGVLLLVDSFE
                     GPMPQTRFVLEKALALHLKPIVVINKIDRPDNRPIEVLDEIYDLFIELDAHEDQLDFP
                     VIYASGRDGWAIKDMKDDRNDLTPLLDSIIEDLPSPPIKKGSVQIQVMTLDYNDYVGR
                     IGIGRVFRGKLKKSEKLSIIKRNGTIHPISIKQLFIFEGLNRVEVEEVSCGDICAIVG
                     IEDVDIGDTIADAENPEPLPIISIDEPTISMTFTVNNSPFYGREGKFVTSRQLRDRLY
                     KELEHNVALRVQETASPDAYRVSGRGILHLSILIENMRREGYELQIREPKVIYKTIEN
                     RKAEPIEVMVVDVPSEFAGKVIELVGQRRGELMKMENKGNMNKIEFHIPSRGIMGLRT
                     KVLTATQGEAVMHHRFYQYEFFKGSIGTTKSGVIISMEEGTATAYAIDGLQDRGKFFI
                     EPGEIVYKGQIIGEHTKENDIEVNVQRGKKLSNMRASGSDKAVKITPAIKFSLEEALE
                     YISDDEMVEVTPKSIRLRKLYLDTNDRKRYNLGKLAQQ"
     gene            complement(2972..>3592)
                     /locus_tag="VF870_13760"
     CDS             complement(2972..>3592)
                     /locus_tag="VF870_13760"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9253307.1"
                     /translation="VQLGDIKNNSTVLIHSAAGGVGIYANRIAKKFNAYTIGTVGSEK
                     KIDLLKREGYDQVIIRGSDFSEKLKQSLDGRNLDIVLECIGGKIFSESFKQMTAGGRI
                     IVYGAAQFSTGNSSPNYIKVLTKYITRPKVDPLSLSDKNKNVMGFNLIYLWDKVDLMR
                     KYLNNILEMNLPKPLIGEVYNFNDLLSAIKKFQSGETMGKVVVRVE"
BASE COUNT         1128 a          671 c          606 g         1187 t
ORIGIN      
        1 tgaaggaaag attgaccttg gagaggttac ggatataaat aaaatttcta ttggattttt
       61 tcaggcttca tcatcgtggg ttgtttttcc aaagtatgtt gaattcttta catcatctga
      121 tggtatagaa tatcagagtg ctggtaaaat tgatattcca tcatcattaa gaaatccgga
      181 ttgggtacaa aaagatttct ttctggattt taaagaagtt aaaggaagat atgtaaaaat
      241 ctttgcaaag aatttgcctg aacttccacc atggcatcct gaagcgggcg gtaaaccctg
      301 gttaatgatt gatgaaataa ttgttgagta aaaatattta gtgcttgatt tacacttaca
      361 tgagctaaaa tatttattta tttcccggtg cggaatgtca tttcgaccgc agggagaatc
      421 tgaattttaa aattatgaaa acagcaatag ttatcggtgc aactggatta gtgggaagtt
      481 ttatcacaca taagcttctt gaagataatc gatatacaaa agtaaaagta tttgtaagaa
      541 gatctctcca gattaataat ccaaagctgg aagaacacat aattgatttt gataaaattg
      601 aattgtggaa agataggtta accggcgatg aactatactc tgcgttgggc acaacaatta
      661 aaaaagctgg cagtaaagac gcacagttta aaattgattt tacatatcaa tacgaagctg
      721 caaaggcagg cttagcaaac ggagtaaaaa cttacttgct ggtctcatct gcgggcgcaa
      781 attccaaatc aggtaatttt tatttgcgga tgaagggaaa tctggatgaa aaagttcagc
      841 aattaaattt cgataagatt tatattttca gaccatcaat acttgcaggc gatagaaacg
      901 aaaagagatt tggtgaatca atcggaataa aaatagccgg aacaattact aaaattattc
      961 cggctctaaa aaagtatcgt cctattgaag cttcaaaagt tgcagaggct atgattaaat
     1021 ctgcaactca aaatttagtt gataaaatca ggatttatca accagaagaa atatttaata
     1081 tttgatctta ttgctgtgca agctttccaa gattatatct ttttctatca ttagtatcaa
     1141 gataaagttt tctcaagcgg atagattttg gtgtaacctc aaccatttcg tcatcagaaa
     1201 tatattctaa cgcctcttcc aaagaaaatt ttattgcagg ggttatctta accgctttat
     1261 cagaacccga agcacgcata ttagataatt ttttaccgcg ctgaacgttt acttctatat
     1321 cattttcttt tgtatgttcg cctatgattt gtcctttgta aactatttcg ccgggctcaa
     1381 taaaaaactt acctctatcc tgcagaccat caattgcata tgcggttgct gtgccttctt
     1441 ccattgaaat tattacacca cttttagtcg tgccgattga gcctttaaaa aactcatatt
     1501 gataaaatct atgatgcata actgcttctc cctgagttgc agttaatact tttgtacgca
     1561 gtcccattat accgcgtgat ggaatatgaa actcaatttt gttcatattt cctttgttct
     1621 ccattttcat cagctcgcca cgacgctgcc caacaagttc gattactttt cccgcaaact
     1681 ctgagggaac gtcaacaacc ataacttcaa taggttctgc ttttctgttt tctatagttt
     1741 tataaattac ttttggttcg cgtatctgaa gttcataacc ttcgcggcgc atattttcaa
     1801 taagaattga taagtgcaga attccacgtc cggataccct gtatgcatcc ggagatgcgg
     1861 tttcctgaac tcttaaggca acattgtgtt ccagttcttt atataatcta tcgcgaagtt
     1921 gacgtgaagt gacaaacttt ccctctctgc cgtaaaaagg agagttgtta acagtaaatg
     1981 tcatactgat agtaggttca tcaatagaga tgataggaag cggctcggga ttctctgcat
     2041 ctgcaattgt atcaccaata tcaacatctt caattccaac aattgcacag atatcaccgc
     2101 aagacacctc ttcaacttca acacgattta atccttcaaa aataaaaagc tgttttatgg
     2161 atataggatg aatggtgccg tttcttttta tgattgaaag cttttccgat tttttcagct
     2221 ttcccctgaa tactctgcca attccgattc ttcccacata atcattataa tccagagtca
     2281 ttacctgaat ctggacagaa cctttcttta ttggcgggga aggaaggtct tcaattattg
     2341 aatcaagcaa aggagttaaa tcgttacgat catctttcat atctttaatt gcccagccat
     2401 ctcttccgct tgcgtaaata accggaaaat ccaattgatc ttcgtgagca tcaagctcaa
     2461 taaataaatc ataaatttca tcgagaactt caattggacg attatcggga cgatcgattt
     2521 tattaataac aactattggt tttaaatgaa gcgctaatgc tttttcaagc acaaatcttg
     2581 tttggggcat tggtccttca aatgaatcaa caagcagtaa aacaccatcg gccattttta
     2641 aaacacgttc aacctcacca ccaaaatctg cgtgacccgg ggtatcaatt acatttattt
     2701 tataatcttt gtagggaatg ctgatatttt tagcaaggat ggtaattccg cgttcacgtt
     2761 caagatcgtt tgaatcaagg aatctttctc taacaacctg atttgttctg aaaacagcac
     2821 attgttttaa tatctgatcc acaagcgtag tcttaccatg atcaacgtgg gcaattatgg
     2881 ctatatttct aatttttttc atcgttctcc aagtgattaa attttgaggt gctaatttac
     2941 gcaagtatgg tcagaagtca gaatgtggtt attattctac acgaaccaca acttttccca
     3001 tagtctcacc ggattggaat ttctttatcg cacttaataa atcattgaaa ttataaactt
     3061 cacctatcaa aggttttggc agattcattt ctaaaatatt gttcaagtat tttctcatca
     3121 gatcaacttt atcccataag taaataagat taaaacccat aacgtttttg tttttatcag
     3181 ataatgaaag cggatcaact tttgggcgtg ttatatattt ggttagaact ttaatgtaat
     3241 ttggagaaga gttgcccgta gaaaactgcg ccgcaccata aacaattatc cttcctccgg
     3301 cagtcatttg cttaaaactt tcagaaaata tttttccgcc aatgcattcc aaaacaatat
     3361 caagatttct tccatcaaga ctttgtttta acttttcaga aaaatcactt ccacgaataa
     3421 ttacttgatc ataaccttct cttttaagaa gatcaatttt cttttcactt cctactgtac
     3481 cgattgtata agcattaaac ttttttgcaa tgcggttagc gtaaattcca actccgccag
     3541 cagcagaatg aattagtact gttgagttat tttttatatc cccaagttga ac
//
LOCUS       DASWBY010000759         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_745853, whole genome shotgun sequence.
ACCESSION   DASWBY010000759 DASWBY010000000
VERSION     DASWBY010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745853"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VF870_13765"
     CDS             complement(<1..183)
                     /locus_tag="VF870_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX9253308.1"
                     /translation="MAKLIVYDADARTGLKAGVDKLANAVKVTLGPKGRNVILEKKFG
                     APTVTKDGVSVAKEIEL"
     gene            complement(216..506)
                     /gene="groES"
                     /locus_tag="VF870_13770"
     CDS             complement(216..506)
                     /gene="groES"
                     /locus_tag="VF870_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9253309.1"
                     /translation="MKLKPLGDRVVVKPAAAEEKTAGGIFLPDTVKEKPVEGTVVAAG
                     PGKVADDGKAIKMEVKVGDKVLYGKYSGTEVTINGEEYLIMRESDIFGILGK"
     gene            669..1460
                     /locus_tag="VF870_13775"
     CDS             669..1460
                     /locus_tag="VF870_13775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9253310.1"
                     /translation="MNNFNIKSELKELIRTIKELDKKVIAVFLAVAVLQTISFYYTSR
                     RFFRANLFEQFQDYSDPYLLEYLYWFIGDFFTLFILGIVIVKFGFKEKLSEYGLQFGD
                     IKVGLSISLIFLSVMIILVWVVSATPAFAEKYPHLASAKSDWNTLFIYEAGMLIYMFA
                     WEFIWRGFMLFGLEKKFGVYAVLMQMIPFVILHNGKPFLETFGAIFGGIALGILALRT
                     RSIYYCIVVHIGVMFSIDLISSLRFRADDYGIGFSSILNILKQIF"
     gene            1469..>1576
                     /locus_tag="VF870_13780"
     CDS             1469..>1576
                     /locus_tag="VF870_13780"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX9253311.1"
                     /translation="MRLSLNIDHIATLRNARGETQPDPVTAALLAEQAGI"
BASE COUNT          458 a          268 c          266 g          584 t
ORIGIN      
        1 aagttcgatt tcttttgcaa ctgatacacc atcttttgta actgttggcg caccaaattt
       61 cttttctaaa attacattac gtccttttgg acctaaagta actttaacag catttgctag
      121 tttgtccaca ccagctttta acccggtgcg tgcatctgca tcataaacaa ttaattttgc
      181 cataatttat ttcctttata ttttctgttt aataattact tgcctaaaat tccgaagata
      241 tcgctctcac gcatgataag atattcttca ccattaattg taacttctgt tccactgtat
      301 ttgccataaa gaactttgtc accaacttta acttccattt taattgcttt gccatcatca
      361 gcaactttgc caggaccagc agcaacaacg gttccttcaa caggtttttc ttttactgta
      421 tcaggtagaa aaattccacc agcagttttc tcttcagcag cggcaggttt tacaacaact
      481 ctgtcaccga gtggttttaa tttgatttcg ctcataatta gctcctttta ttttgttatt
      541 agaatttgat tagcactctt aaagaacgag tgctaatata cactcgtttt aaaattttgt
      601 caagaaattg agcttttgat gtgatataaa ttgcttaaat ttgaagcgaa aataaacatt
      661 attaaagatt gaataatttt aatataaaat ccgagctaaa agagcttatc cgaactatta
      721 aagagctgga taaaaaagtt attgctgttt tcctggctgt cgcagtactt caaaccatat
      781 cgttttacta tacctcaaga cgatttttcc gtgcaaattt gtttgagcaa tttcaagact
      841 attccgatcc atatttgctg gaatatctct actggtttat cggagatttt ttcactctct
      901 tcattctcgg tattgtgatt gtaaagttcg gatttaaaga aaaactttct gagtacggat
      961 tacagtttgg agatattaaa gttggattat caatttcttt aatttttctt tctgttatga
     1021 ttattctggt ttgggtggtt tcagcaacac cagcgtttgc agaaaagtat ccgcacttag
     1081 ccagtgcaaa atctgattgg aatacattgt ttatttatga agcaggtatg ctgatttata
     1141 tgtttgcatg ggaatttata tggcgtggat ttatgttgtt cggattagaa aaaaagtttg
     1201 gtgtttatgc tgtgttaatg caaatgattc catttgtgat tttgcataac ggtaaacctt
     1261 ttctggaaac attcggggca atttttggag gaatagcact tggcattctt gcattaagaa
     1321 ctcgttcgat ttattattgt atcgttgttc atataggtgt aatgttttcc atagatttaa
     1381 tttcatcttt acgttttcgt gctgatgatt acggaatagg attttcatct atcttaaata
     1441 tattaaagca aattttctga ggtatgaaat gagattatca ttaaatatag atcatatagc
     1501 aacattaaga aacgccaggg gagaaacaca acccgatcct gtaaccgctg cattattagc
     1561 cgagcaagca ggtatt
//
LOCUS       DASWBY010000760         3736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_595404, whole genome shotgun sequence.
ACCESSION   DASWBY010000760 DASWBY010000000
VERSION     DASWBY010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595404"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VF870_13785"
     CDS             complement(<1..905)
                     /locus_tag="VF870_13785"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX9253312.1"
                     /translation="MTSVKKNKFGNVGMIINYGISIILAVVFLYIAFYNVDFNEVLNI
                     VSHANLFWVLIFIIVTMLGHYFRAVRWKYILNSVKPNTSMTNLFASLMVGYGVNCVTP
                     KLGEVTRAVLIGKWEGLSRSSMFGTVILERIIDIISMGGSIIIAIFLTSVSLSEKFPW
                     LITSLYIMGAVIVLSIVFLSLILRSREKFSKIILKLVGKFSQKLAERIAYIFTMLAEG
                     FASLKGIKNYFWTIFWTIAIIIIYAFGSYVGLLMLDMQNFQPITFGMGWIIMSISAIG
                     VIIPTPGSTGSYHTLAKSTLVLIFG"
     gene            complement(892..1488)
                     /locus_tag="VF870_13790"
     CDS             complement(892..1488)
                     /locus_tag="VF870_13790"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX9253313.1"
                     /translation="MKFLISLIVLFYSTVGFTQSAESVLKSLQNKFDSITDLTTDVAQ
                     KSNGKSSLSGKMYFKKENKLRIELGNQTIVADGKTSWNYNKKDKKVIITDYDEAGSGL
                     LSINYLIYQYPSECDLSLSSEGSSQILNLKPKSKKNNPGDVKLYITNENLIDKAVISN
                     AASGTMEVSFSNYKLNQNLSDSKFSFTAPEGTTVVDLR"
     gene            complement(1528..>3736)
                     /locus_tag="VF870_13795"
     CDS             complement(1528..>3736)
                     /locus_tag="VF870_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX9253314.1"
                     /translation="HFSLFLVKATFGYFSISIPLVLFLWGISFFKKIDFKFRLHVSNF
                     LLLSSIILASFFGVLQVNYGLFSSTYELSGFIGNHLGNFLSGLVGGIGSILILAGSFI
                     TLLIFALDINIQSIINFFKEVFSVSDEETKKDEKKTSDDTDSKENLEKIKKLGTDKKK
                     KKETQKEEELSVAELMEKEAEEQTKIRIIRKDDVPVTAAENEVLNEEVKKVNLEKTGE
                     LPEKKEKTETEDEQKLPNQWEENIEYKMPGTDLLDPAPEENYQVAEEELTHNAGLLKE
                     KLKLFDIEIKDISVTPGPVVTLYEIVPAPGVKISKIVGLENDIALALAARGIRIIAPI
                     PGKGAIGVEIPNAVASLVNARAVIGRINESDAELPLALGKTISGDVYVADLAKMPHLL
                     VAGSTGSGKSVGINMIVSSLLYTKHPSEVKFVMVDPKKIELSFYNKLRKHYLAVSPDL
                     DEEIITNPQNAILALKSVEYEMERRYDKLAKAGVRNIVDYNKKVKDPKTRPHDTDKIK
                     HHPIPYVIVIVDELADLMITAGKEVEEPITRLAQLARAVGIHLVLATQRPSVNVITGV
                     IKANFSSRIAYQVATKIDSRTILDMNGAEQLLGRGDMLFLPTGSPKPIRIQNAFISTE
                     EVERITNYVYSQQAYSKPYFLPSLYDKKKESSSGVSSDLDPMFEDAAKVIVRHQQGSV
                     SLLQRRLKLGYSRAARIVDQLEEAGIVGPNDGSKARSVLVENEEQLETILRNL"
BASE COUNT         1170 a          692 c          579 g         1295 t
ORIGIN      
        1 aatccaaaaa ttaaaactaa agttgatttt gcaagagtat gatatgagcc tgtgcttccg
       61 ggtgtaggta ttatcacccc aattgcgctt atactcataa ttatccatcc cattccaaat
      121 gttattggtt gaaagttctg catatcaagc attaataatc ctacataaga accaaaagca
      181 tatataataa ttatcgctat agtccaaaat atcgtccaga aataattttt tatccctttt
      241 aagcttgcaa aaccttcagc aagcattgta aatatgtaag caattctttc agcaagcttt
      301 tgagaaaatt ttccgaccaa ttttaaaatt attttagaaa atttttctct tgatcttaag
      361 atcaaagaaa gaaaaacaat agataaaact ataactgcac ccattatgta taatgaagtt
      421 ataagccagg gaaatttttc tgaaaggctt acacttgtta aaaatattgc aattataatt
      481 gatcctccca tagatattat atctataatt cgttcaagta taactgttcc aaacattgaa
      541 gatctagata gtccttccca ctttccaata agaacagcgc gggtaacttc gccaagtttt
      601 ggtgtaacgc aattcactcc gtatcccacc ataagcgaag caaaaagatt tgtcattgat
      661 gtgttgggtt tgacggaatt tagaatgtac ttccatctaa cagcacgaaa ataatgcccc
      721 agcattgtaa caattataaa aataagaacc caaaataaat ttgcatgaga aacaatatta
      781 agaacttcgt taaaatcaac attataaaat gcgatataca gaaatactac agcaaggatt
      841 atagaaatgc cataattaat tatcattcca acattgccaa atttattttt cttaacggag
      901 gtcaactaca gtggttcctt ccggggctgt aaatgaaaat ttggaatctg ataaattctg
      961 atttagctta taatttgaaa aggatacttc cattgttccg gaagcagcat ttgatataac
     1021 tgctttgtca atcaaatttt catttgtgat gtatagtttt acatcaccgg ggttattctt
     1081 ttttgatttt ggttttaaat ttaaaatctg gctgctgcct tcagatgata aacttaaatc
     1141 gcattcggat ggatattgat aaataagata attaatcgac aataatcctg aacctgcttc
     1201 atcataatca gttattatta cttttttgtc tttcttgttg taattccagg aggtctttcc
     1261 atccgcaaca atggtttgat ttccaagttc tatcctaagt ttgttttctt ttttaaaata
     1321 catcttaccc gaaagacttg atttaccatt ggatttctgt gcgacatcag ttgtaagatc
     1381 ggttatagaa tcaaatttat tctgcagtga ttttaaaact gattcggcac tttgcgtaaa
     1441 tccaactgtt gaataaaata atactataag gcttattaaa aatttcatgt caaactctaa
     1501 tttttaaaaa attgtttaat gtttggacta tagattacgc agtattgttt caagctgttc
     1561 ttcattttca acaagtacag aacgggcttt acttccatcg tttggaccaa caataccggc
     1621 ttcctcaagc tgatctacaa tccttgctgc acgtgaataa ccaagcttta accttctttg
     1681 aagcaaagac acagacccct gctgatgacg tacaattacc tttgcagcat cttcaaacat
     1741 aggatctaaa tctgacgaaa caccgcttga agattctttt ttcttatcat ataaagaagg
     1801 caaaaaatat ggttttgaat atgcctgttg agaataaaca tagtttgtaa ttctttcaac
     1861 ttcttcagtc gatataaatg cattttgaat tcttattggc ttcggtgaac ccgttggaag
     1921 gaataacata tcgcctcttc caagcagctg ttctgcacca ttcatatcaa gaattgtacg
     1981 tgaatcaatt ttggttgcaa cctgatatgc aatccttgaa ctaaaatttg ctttaattac
     2041 acctgtaata acatttactg atggccgttg tgttgcaagc acaagatgaa ttccaaccgc
     2101 gcgagcaagc tgtgccaatc ttgtaatagg ttcttcaact tctttacccg ctgttatcat
     2161 taagtcagca agttcatcaa caattacaat tacatatgga atcgggtgat gttttatttt
     2221 atctgtatcg tgcggtctgg ttttaggatc tttaactttt ttattgtaat caacaatgtt
     2281 tcttacgccg gctttagcaa gtttatcata acgtctttcc atttcgtatt caacgctctt
     2341 taaagctaaa attgcatttt gcggatttgt aattatctct tcatcaagat caggagaaac
     2401 tgccaggtaa tgtttacgga gtttattata aaaagatagt tctatctttt tgggatcaac
     2461 cattacaaac ttaacttctg aaggatgttt agtataaagt aaactgctta ctatcatgtt
     2521 tattcccaca ctctttccgg agccagttga tccggcaact aataagtgcg gcatttttgc
     2581 gagatcagca acataaacat ccccgctaat agttttgcca agagctaatg gaagctctgc
     2641 atcagattca ttaattcttc cgattacagc acgggcattt acaagtgatg ctactgcatt
     2701 tggaatctca acgccaattg ctcccttacc tggaattggt gcaatgattc tgattccgcg
     2761 tgccgctagt gcaagagcaa tatcattttc taatccaact atcttgctaa tcttaacacc
     2821 aggcgcaggt acaatttcgt acaatgttac aaccggtccg ggagtaacag agatatcttt
     2881 tatttcgata tcaaaaagtt ttaatttttc tttaagcaat cccgcgttgt gtgttaactc
     2941 ttcttctgct acctgataat tttcttccgg tgccggatca agtaaatcag taccgggcat
     3001 tttgtattca atattttctt cccactgatt aggcaatttc tgctcatctt cagtttcagt
     3061 tttttctttc ttttcaggaa gttcgcctgt tttttccaga ttaacttttt ttacttcttc
     3121 attaagaact tcattttcag ccgcggtaac cggaacatca tcttttcgga taattctaat
     3181 tttcgtttgt tcttcagctt ctttttccat tagttcggca acggataatt cttcctcttt
     3241 ttgtgtttct tttttcttct ttttatcagt gccaagcttt ttaatttttt caagattttc
     3301 ttttgaatca gtatcgtcag aagttttctt ttcatccttc ttagtttctt cgtcgctaac
     3361 tgaaaatact tctttaaaga aattgatgat actttgaatg tttatatcca aagcaaaaat
     3421 taagagagtg ataaaagatc cggcaagaat tagtatactt ccaattccac cgactaatcc
     3481 gcttaaaaaa ttacccaaat gatttcctat aaatcctgat aattcataag tagaagagaa
     3541 taaaccataa tttacctgaa gcacaccaaa aaaggacgct aatatgatcg agcttaataa
     3601 caggaagttt gatacgtgaa gtcggaattt gaaatcaatt tttttaaaaa atgaaattcc
     3661 ccataagaaa agtacaagtg gaattgagat tgagaaatat ccaaaggttg ctttaacaag
     3721 aaatagagag aaatgc
//
LOCUS       DASWBY010000761         4932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_745896, whole genome shotgun sequence.
ACCESSION   DASWBY010000761 DASWBY010000000
VERSION     DASWBY010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745896"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1273
                     /locus_tag="VF870_13800"
     CDS             83..1273
                     /locus_tag="VF870_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253315.1"
                     /translation="MIKNFFPVFLFAISIFFIVSCNDSPTDLGTTFLSQDGVEVRKLD
                     SSIDTISQSSYSYKNVLPLGGSTQLLLGKSDNITSNVLLSFVFAIPDSIKQELEARNL
                     TVIDSYVEMYKNYSFGDSNAAFDYAVYKVNEKWSSSTFTADSFAFLDYDNSIDLSSNR
                     TTENDTIYSFHLDTTLTSSWLQNYIDTTVASNHGILLSPTTNTQKVLGFTAFNVNAQN
                     DPRLRIVVQKPGAYIDTLRGFVSSDLSVVLGDLPNVGTENIVIQSSLTAEAKLFFDFS
                     VVPEDVVINLATLTFTVDTLLTKIGSSHANRLSVYLLDDSSTNKINTDYASTLERKGS
                     TFTGDVTSILRAWNNKVENQGVLIKSTLELWGLEIYALKGSNAPLIEQRPRLEIVYSK
                     GKNH"
     gene            1273..2517
                     /locus_tag="VF870_13805"
     CDS             1273..2517
                     /locus_tag="VF870_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253316.1"
                     /translation="MKNISLLGSLVIIFSSTIIAQSNSVYTRAGVGDLEYGYSAKMIG
                     IGNIGSTQLDPDHLMVTNPASWSVLSRTRIEFGIGYRGALLSDSQQENYTSETEFKGV
                     TFGIPVSREYGIGAVIGLVPYSRISYSAEKYYPTDDIIPGYKLFYEGKGGLSKIFLGS
                     SFFLPLGFAAGVTLDYYFGNQNYATTISFDDPRYISTKYENTRRSTGFGTTVGLISSN
                     LAREFKIGGLSDLRIGLSYNYVGNLNTDSLFTQTSLFLVDSITNTKAEMKIPPRITGG
                     ISFAIEESYNFYLDMLFQPMSDYSFNGQPDPNLKDETRYTASFEFKPKRSIGMTTLEQ
                     IIWRMGLSYEQTQYTFNGEDINQFSVYGGLSYPLGVDNTIDFAVEYSNKGKTENNLLH
                     EHAIKIYLGLSFGELWFLRYDK"
     gene            2545..3777
                     /locus_tag="VF870_13810"
     CDS             2545..3777
                     /locus_tag="VF870_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253317.1"
                     /translation="MKTLLQTIAITLVIFASTNLAQNADSMKVLTDYSLFSEYHKNKD
                     FDSALPYGWSVIEANPVKFSKWIYYKMEECLWAVHDSSSSTPEQVKAVQDTILYFYDL
                     AIKYRDQDKGYFQARKALVSEIWLNLPASVTIPAYEQAIAWQPELSSFYYNRLGQLYI
                     NNDDGTNGYKEKAIDLYTVLVEREPENPVWGNIITDLFGNIDDRLKFQKDAWEKDPQN
                     EKKAKALAATAIQASEWAQAVAPLEFLVSKDPTNVGYLTQLGNVYNKLENAVKSEEVF
                     SKLISLDPGNKDHYFYFGKALMDAGKYSQARTQFLKASEVGNGWALPIFYEGFLYEQS
                     ASGCTDFDRKLVYLLAQNTYRKALSMDPNLNEAKDRISALSGAVPTKEDYFFRNLKSG
                     TVLPITCVSWIGKSVTVP"
     gene            3874..>4932
                     /gene="glyA"
                     /locus_tag="VF870_13815"
     CDS             3874..>4932
                     /gene="glyA"
                     /locus_tag="VF870_13815"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9253318.1"
                     /translation="MESYLKKDAEIFKVLQSEIGRQTNKLELIASENFVSPAVLEAAG
                     SVLTNKYAEGYPGKRYYGGCEFVDQAEDIARDRLKKLFGAEYVNVQPHSGSQANMAVL
                     MTFLKPGDKFLGLSLAHGGHLTHGSPVNFSGKLYEAVGYELDEETGLLDYNKIADLAK
                     KEQPKLIITGASAYSRDWDYKKFREIADSVGAFLMCDMAHPAGLIAKGLLNNPLEYCD
                     IVTSTTHKTLRGPRGGIILIGKDKENPWGITTPKGDRVKMLSELIDSMVMPGIQGGPL
                     MHIIMAKAVAFGECLSDSFKDYVMQVRNNAQALSAKLMEYDFNVVSGGTDNHLMLIDL
                     SNKNITGKQAEIALEHAGI"
BASE COUNT         1655 a          752 c          936 g         1589 t
ORIGIN      
        1 aataatttcc caaactttgt agctgaaaat ttaactgaag cctgtaagtt tataattaat
       61 gattccactg gagtagtaat tagtgattaa aaattttttc cctgtgttct tatttgcaat
      121 ctctattttc tttatagtta gctgcaatga ttccccaaca gatctaggaa caacatttct
      181 ctctcaagat ggtgtagaag taaggaaatt ggattcttca atagatacta ttagccaatc
      241 atcttatagt tataaaaatg ttttacctct cggcggctca acacaattgc ttttaggcaa
      301 atctgataat attacttcaa atgtactttt gtcttttgtt tttgcaatac ctgattctat
      361 aaaacaagaa ttggaagctc gaaatctgac tgttatagat tcgtatgttg aaatgtacaa
      421 gaattattca tttggagatt ctaatgcggc atttgattat gcagtctata aagttaacga
      481 aaaatggtcg tcatctacat ttactgcaga cagctttgca ttcttggatt atgataattc
      541 aatagattta agttcaaacc gaacaactga aaatgacact atatattctt ttcatttaga
      601 taccacttta acttcatcgt ggctgcaaaa ttatattgat actactgtag cttcaaatca
      661 tggtattctg ctttctccaa caactaatac tcagaaagtt ctgggattta cggcgtttaa
      721 cgttaatgca caaaatgatc ctagattaag aattgttgtg caaaaacccg gtgcttatat
      781 agatactctt aggggattcg tttcctctga tttaagtgtt gtactaggtg atttaccaaa
      841 tgttggaact gaaaatattg taattcaatc cagtttaaca gcagaagcta aattattttt
      901 tgatttttca gttgtgccgg aagacgtcgt tattaattta gccactctta catttactgt
      961 ggatacatta ttaacaaaaa tcggcagcag tcatgctaat agattaagcg tttatctttt
     1021 agatgattcc tcgaccaata aaattaatac cgattatgct agtacacttg aaagaaaagg
     1081 ttcaactttt actggtgatg ttactagtat ccttagagcc tggaataaca aagtagaaaa
     1141 tcaaggtgta ttaatcaaat ctactttgga actttgggga ttagaaattt atgcactaaa
     1201 aggaagtaat gctcctttaa ttgagcaaag gcctagactt gaaattgtct attctaaagg
     1261 gaagaaccac taatgaaaaa tatatccctt ttaggcagtc ttgttataat tttttcttca
     1321 acaattattg cacaaagtaa ttcagtctat actcgtgctg gtgtaggtga tcttgagtat
     1381 ggatattctg ctaagatgat tggcattgga aatattggat cgacacaatt agatccggat
     1441 catctaatgg ttacaaatcc tgcaagctgg tctgtattat cccgaacaag aattgaattt
     1501 ggaattggtt acagaggtgc gcttttatct gatagccagc aggaaaatta tacaagtgaa
     1561 accgaattta aaggagttac ttttggaatt cctgtaagca gagaatatgg aattggagca
     1621 gtgataggtt tagtgcctta ttccaggata agttacagtg cagaaaaata ttatcctact
     1681 gatgatatca ttcctggtta caaattattt tatgaaggta agggcggatt atcaaaaata
     1741 tttcttggct catccttctt tttaccttta ggttttgctg ctggtgtaac tctggattat
     1801 tattttggta atcagaatta tgcaacgact atcagttttg atgacccaag atatataagt
     1861 accaaatatg aaaatacccg tagatcgaca gggtttggta ctactgtggg attaatttct
     1921 tcaaatttag caagagaatt taaaattggc ggactaagtg atttaagaat tgggttatca
     1981 tataattatg ttggtaatct taatactgat tctcttttta cacaaacatc gcttttttta
     2041 gtagacagta ttacaaatac taaggctgaa atgaagattc caccaagaat tacaggcggc
     2101 ataagttttg ccatcgaaga atcatataac ttttatttgg atatgttgtt tcaaccgatg
     2161 agcgattata gttttaatgg tcagcctgat ccaaatttga aagatgagac ccgatatacc
     2221 gcatcatttg aatttaagcc caaaagatca atcgggatga caacattgga acaaataatt
     2281 tggagaatgg gtttaagtta tgaacaaact caatatacct ttaatggaga agatataaac
     2341 caattttcag tttatggagg gttatcctat ccattaggag ttgataatac tattgatttt
     2401 gcagttgaat attcaaataa aggaaagact gaaaataatt tattgcatga acacgcaata
     2461 aagatttatc tcggtttaag ttttggtgaa ctctggtttc ttagatatga caaatagatc
     2521 actaatatca taaaggttgg aaaagtgaaa acattattac aaacaatcgc aattacactg
     2581 gttatttttg cttcaacaaa cttagcacaa aatgctgatt caatgaaagt gctgacagat
     2641 tacagtctgt tttctgagta tcataaaaac aaagactttg atagtgcttt accttacgga
     2701 tggagtgtaa tagaagccaa tcctgtaaaa tttagcaaat ggatatacta taaaatggaa
     2761 gaatgtcttt gggcagttca cgattcttca tcttcaactc ctgagcaggt aaaagctgta
     2821 caagacacta ttttatattt ttatgattta gccattaaat atagagatca ggataaagga
     2881 tattttcagg ctcgtaaagc tctagtatct gagatatggt taaatcttcc tgcttctgta
     2941 acaattcccg catacgagca ggcaatcgct tggcagccgg aattatcatc attctattac
     3001 aatagattag gccaattgta tatcaacaac gatgacggaa caaatggtta taaagaaaaa
     3061 gcaattgatc tttacacagt tttggtagaa cgtgaaccag aaaatccagt ttggggaaat
     3121 attataaccg atttatttgg aaacatagat gatagattaa aattccagaa agatgcgtgg
     3181 gaaaaagatc cgcaaaatga aaagaaagct aaagcccttg ctgcgacagc aattcaagca
     3241 agtgaatggg cacaagcagt tgcaccactg gaatttttag taagcaaaga tcctacaaat
     3301 gttggttact taactcagtt gggtaatgtc tataataaac tagaaaacgc cgtaaaatca
     3361 gaagaagtat tttctaaatt gattagttta gacccaggta ataaagatca ctatttttat
     3421 tttggcaaag cgttgatgga tgcgggaaaa tattctcaag cccgaactca gtttttaaaa
     3481 gctagcgaag ttggaaacgg ctgggcttta ccaatttttt atgaaggctt tttgtacgaa
     3541 cagtctgcaa gcggctgtac tgattttgat agaaagttag tttacttgtt agctcaaaat
     3601 acttatcgaa aagctttgag tatggatcca aatcttaacg aagcaaaaga tagaataagt
     3661 gctttatcag gtgctgtccc gacaaaagag gattatttct ttagaaattt aaaatccggc
     3721 acagtgctgc caattacttg tgttagctgg ataggtaaaa gtgttactgt cccttaactt
     3781 ttgttcataa tttttaattt ggaaaggttt cttgagattt caggaaacct tttctaattt
     3841 tgactaattc attcatcaaa ataagcggta aatatggaat catatctaaa aaaagatgct
     3901 gaaattttta aagttcttca gtctgaaatt ggaagacaaa ccaacaagtt agaattaatt
     3961 gcaagtgaaa attttgttag tcctgctgta ttagaagctg ccggttcggt tttaactaat
     4021 aagtatgcag aaggatatcc cggtaaaaga tattatggcg gctgtgaatt tgttgatcag
     4081 gctgaagata ttgcacggga tagacttaaa aaattatttg gtgctgaata tgtgaatgta
     4141 cagccacata gcggctcaca agctaatatg gctgtgttga tgacatttct aaagcctggt
     4201 gataaatttt taggattgag tcttgcacac ggcggacatc taactcatgg ttcacctgtt
     4261 aacttttcag ggaaattgta tgaagcagtt ggttacgaac ttgacgaaga aacaggttta
     4321 cttgattata ataagattgc cgaccttgct aaaaaagaac aaccaaaatt aataattacc
     4381 ggtgcaagtg catactcaag agattgggac tacaaaaagt tcagagaaat tgctgattct
     4441 gttggtgcat ttctaatgtg cgatatggct catccagccg gattgattgc taaaggattg
     4501 ttgaataatc cactcgaata ttgtgatatt gttacatcaa caactcataa aactttacga
     4561 ggaccaagag gtggaattat tcttattgga aaagacaaag aaaatccctg gggaataaca
     4621 actccaaaag gtgatagagt aaaaatgctc tctgagttga tcgacagtat ggttatgccc
     4681 ggtatccagg gcggaccatt aatgcacata ataatggcta aggctgttgc gtttggtgaa
     4741 tgcctttcag attcatttaa agattatgtt atgcaggtta gaaataatgc ccaagcatta
     4801 tcagcaaaat taatggagta tgattttaat gttgtatccg gcggaactga taaccattta
     4861 atgttaattg atctatcgaa taaaaatatt accggtaagc aggcagaaat agcattagaa
     4921 catgcaggta ta
//
LOCUS       DASWBY010000762         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_182955, whole genome shotgun sequence.
ACCESSION   DASWBY010000762 DASWBY010000000
VERSION     DASWBY010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182955"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1606
                     /locus_tag="VF870_13820"
     CDS             245..1606
                     /locus_tag="VF870_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9253319.1"
                     /translation="MKITEVFNLIQVFTKGGFYIQYQLPDFIGQTSFASKNWKPYHIC
                     FSKKREKKKTQQITARLNKILSDIKKGIQKQITDFLGLLDKIKDRVSKYIPRLKTAFC
                     KRYDGSKITETEKIAYEFYPDYKGDIRHLYLFYDWNKVDEIEKKHADKIGDYPLRTIL
                     KTQIFMCKRRVRTYSDLIEELRENPELAKVCELNPNCIPDRKVFSRAADRMGIETFRQ
                     IAIDMTKECLFLGLIKGRMVGVDGSLIKSNTSTHKNEETEEYTDKSAGLYVRGNYLKG
                     IGHLVYKLADLEYGLPMLVQRYKGSANENPLLIDIIENFHEVYGFYPEIMSVDKGMDS
                     EKNNEFCKDKGIGAYIQARDFGNKELIKTEKGKTFRPEYVEITDPRVLERIANRRSEC
                     EREFSRDKWGYRRDRMPNRGDKEAEFFMLITMITTLLTAITAFCVGRADLIRSTSAFK
                     RLD"
BASE COUNT          688 a          265 c          344 g          513 t
ORIGIN      
        1 ataacatttt cagactaaat ccagcagcag ataatttttt tgggaattta caattgagga
       61 ttgggaatga agtttaactg aagacatttt taaattataa agtattattt accatttttt
      121 atcataaaaa tttgtaatat aacttaaaat tagatttaaa ggtatataaa tacttttaga
      181 ttttaaacaa ataataaagc ttattttgga ttaaatacct ctattattaa ccaaaatcca
      241 aaatatgaaa ataacagagg tatttaattt gattcaagtt tttacaaagg gcggatttta
      301 catccagtat caattacccg attttatcgg gcagacatcc tttgcgagca aaaattggaa
      361 gccatatcat atatgctttt ccaaaaaaag agaaaaaaag aaaactcaac aaataacagc
      421 acgactgaat aaaattctca gcgacattaa aaaaggcatt caaaagcaaa taactgactt
      481 cctgggatta ctggataaaa taaaagatag agtaagtaaa tatataccta gactgaaaac
      541 ggcattttgt aagagatatg atggatctaa gatcacagaa accgaaaaaa tagcatatga
      601 attttatcct gactacaaag gcgatattcg ccatttgtat ctattttatg actggaacaa
      661 ggtagatgaa atcgaaaaaa aacatgcaga taagattgga gattatccct taagaacgat
      721 cctaaaaaca caaatattca tgtgcaagag acgtgtaaga acctattctg accttattga
      781 agaattgaga gaaaatccag agctggcgaa ggtctgcgaa ttgaatccaa attgcattcc
      841 agatagaaaa gtattcagca gagccgctga ccgaatgggt attgaaactt ttcggcagat
      901 tgccatcgat atgacaaaag aatgtttatt tcttggttta ataaaaggaa gaatggtcgg
      961 agtcgatgga agcctcataa aatcaaatac cagtacccat aaaaatgaag aaacggaaga
     1021 atatacagac aaatctgcag gattgtatgt aagaggtaat tatctcaaag gtatagggca
     1081 tcttgtttat aaactcgcag accttgaata tgggcttcca atgctggttc aacgttataa
     1141 aggaagtgca aatgaaaacc ctttgttaat tgatattatt gaaaattttc atgaagtgta
     1201 tggattttat cctgaaatca tgtctgttga taagggaatg gatagtgaaa agaacaacga
     1261 gttctgtaag gacaaaggaa taggagcata cattcaggca agggattttg gaaataagga
     1321 gcttatcaag actgaaaaag gtaaaacgtt tcgtcccgaa tacgtagaaa ttactgatcc
     1381 acgtgttctt gaaagaatcg ctaacagaag aagtgaatgc gagagggagt tcagtcgaga
     1441 caagtggggt tatagacggg atagaatgcc taacagggga gacaaggagg ctgagttttt
     1501 catgttgatc acgatgatta ctacattact tacagctatc acagcatttt gtgtaggaag
     1561 agctgatctg ataagaagta caagtgcttt caagaggctt gactgaaaaa gagccattac
     1621 aaatttttag ggtaaattaa ggcaatatat atcgtaaatt tgaaaataga ggattaaatg
     1681 ggttcacgcc caatcctcta ttgcaacacc agatccgtca taacttatcc aatctccgga
     1741 attaagcaat ttaaaaattc ttccattctg tgtattagcg ttaatttctc caataatcca
     1801 aatttcgtta
//
LOCUS       DASWBY010000763         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_329271, whole genome shotgun sequence.
ACCESSION   DASWBY010000763 DASWBY010000000
VERSION     DASWBY010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329271"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VF870_13825"
     CDS             <1..574
                     /locus_tag="VF870_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9253320.1"
                     /translation="RINELGRESLGIVCDISNEDQVNNLIDVTVKKFGRINVLINNAG
                     IGSMRPVYATSLESFEKVLKVNLTGTFLCTKHVWQHMKNSGGGSIINVSSLGGLVGYP
                     MLSAYCASKWGQIGFTKACAEEGKHDKIRVNAIAPGKADTAIRSKIKEDKTKMLTPED
                     QVDACVFLASDESRYITGQVISLEWFGEDK"
     gene            799..1602
                     /locus_tag="VF870_13830"
     CDS             799..1602
                     /locus_tag="VF870_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253321.1"
                     /translation="MKTLSILFFIVLFSPEIYLFSQQQNNPFVTSHFIGEKLDRIEEN
                     YFHLFPAVTNFQEATFFLNQDSSLTLNLKYKQRDQLIDTTMLYMYSLYQLRNRINQVV
                     LEDIRENKVKEIKLSNTDTTYAGTVYSFDGKQIRLIKKGYTNSPNNYNDQNYLPQFNY
                     SDIRTVTIGESSTAVKIVGSLLGMLVGGLVGFALTPEPRQPETFPEAIFLPMEEASKS
                     MANTFIGLGIGGLLGFFIGGAIDVPVDYDVMSPGTKTIIYDNSLLSKRK"
     gene            1688..2323
                     /locus_tag="VF870_13835"
     CDS             1688..2323
                     /locus_tag="VF870_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253322.1"
                     /translation="MKKIFLALMLILVSSLTAQVRNISERQYFRQSDWEVASSFAIGL
                     SKTTYESTSQNYYAGGSSDEQFYSEISLAIGFFILEGLSIEPELDYNFFQDDATFSMI
                     ANISYTLHIPKKSIYPFFKVGYGKSGFRGYSYSPYPDNSEGLFGSLNANLINASAGLK
                     IIQSSSFAMILELNYKYITFNQTVSTDYAEPYNVDTKTSALSIKIGGSFLL"
     gene            2345..>2536
                     /locus_tag="VF870_13840"
     CDS             2345..>2536
                     /locus_tag="VF870_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253323.1"
                     /translation="MKAFLIFYITACNRILILLLSLSLILSTSCSNPRIERSTSPPDE
                     MINERSSAEVPYLKLHMKNG"
BASE COUNT          854 a          403 c          470 g          809 t
ORIGIN      
        1 gagaattaat gaattgggca gagaaagcct gggaattgta tgcgatattt caaatgagga
       61 ccaggttaac aatcttattg atgtaacagt aaaaaagttc ggcagaatta atgttctgat
      121 aaacaatgct ggcatcgggt caatgcgtcc cgtttatgcg acatctctag aatctttcga
      181 aaaagtttta aaagtaaatc ttacaggaac atttctttgc acaaagcatg tttggcagca
      241 tatgaaaaat tccggtggcg gttcaattat aaatgtttct tctttgggtg gattagtggg
      301 atatccaatg ctttcggctt attgcgcaag taaatgggga caaatcggtt ttactaaagc
      361 ctgcgcagag gaaggaaaac acgataagat tcgtgttaat gcaatagcac cgggcaaagc
      421 tgatacagct attcgttcaa aaataaaaga ggataaaact aaaatgctta cacctgaaga
      481 ccaggttgat gcatgtgttt ttcttgcctc agatgaatca agatatataa caggacaagt
      541 catctcgcta gagtggtttg gagaagataa atgaggagta ttgtaatgga ataagcaatc
      601 attgctaatt attcagggaa tctcatcaaa acttttcttc ttttcaccca ctcttttttc
      661 caaattgtat aggattttac atagaattgg gatttaagca aaaaagatat atgctccgaa
      721 tttgactttt tacaatttca tgacaactct gtaattagcc acaactatat ttctaacaga
      781 atatcagaga ggtgtgactt gaaaacgctg tcgatcttgt tttttatagt tcttttctcc
      841 ccggaaattt atttgttttc gcagcagcaa aacaatccat ttgttacaag tcattttatt
      901 ggtgaaaagc tggatagaat tgaagaaaat tatttccatt tatttcctgc agttacaaat
      961 tttcaagaag caactttctt tttaaatcaa gacagttcac ttacactaaa ccttaaatat
     1021 aaacagagag atcagcttat agatactaca atgttgtata tgtattcttt atatcaatta
     1081 agaaatagaa ttaatcaggt tgtattggaa gatataagag aaaataaggt aaaagaaatt
     1141 aagcttagca atacagacac tacttatgca gggactgtct attcgtttga tgggaaacaa
     1201 attagattaa ttaaaaaagg atatactaat tcacctaata attacaacga ccagaattat
     1261 ttaccccaat ttaattattc tgatataagg accgtgacaa taggcgagag ttcaactgct
     1321 gtaaaaattg tgggcagctt actcggaatg ttagtcggag gattagtagg ttttgcttta
     1381 actccggaac cacggcagcc ggaaacattt cctgaagcaa tttttttacc aatggaagaa
     1441 gctagcaaat caatggcaaa tacttttatt gggctgggaa ttgggggttt actaggtttc
     1501 tttattggtg gagctattga tgttccggta gattatgatg taatgagtcc aggaaccaag
     1561 acaatcattt atgataacag tttactatca aagcgtaaat gagactttta ctaattaatt
     1621 tttacaaatt catgacatct ttattgttct actttattag tatgtattcg aaaaaggatt
     1681 ggaagttatg aaaaaaatat ttttggcctt aatgttaata ttagtgtcct cactcaccgc
     1741 gcaagtaagg aatatttccg aaagacaata tttccgacaa tccgattggg aagttgcaag
     1801 ttcttttgca attggactga gtaagacaac ttacgagagc acttcacaaa attattatgc
     1861 aggtggaagc agcgatgagc aattctattc agagatttcc cttgcgattg gtttttttat
     1921 tttggaggga ttatcgattg aaccagaact tgattataat ttttttcaag atgatgccac
     1981 cttttctatg atagctaata tttcctatac actgcacatt ccaaagaaaa gtatttaccc
     2041 gttttttaaa gtcggctatg gaaaaagtgg attcaggggt tatagttaca gtccctatcc
     2101 tgataactca gaaggattgt ttggttcgct taatgcaaat cttataaatg caagtgcagg
     2161 attaaaaata attcaatcct catcatttgc tatgatatta gaattaaatt ataaatacat
     2221 tactttcaat caaacagtaa gtacagatta cgccgagcca tataacgtcg atactaagac
     2281 ctctgcacta tcaataaaaa ttggcggttc ttttttacta taactcataa aagtgaggaa
     2341 cctgatgaaa gcgtttctaa ttttttatat aactgcctgc aacagaatat taattctact
     2401 gctgtcactc agcttgattc taagcacaag ttgttcaaat ccaaggatag aaaggtcaac
     2461 atcccctccg gacgaaatga ttaatgaaag aagttctgca gaagttccct atttgaaact
     2521 gcatatgaaa aatggc
//
LOCUS       DASWBY010000764         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_627548, whole genome shotgun sequence.
ACCESSION   DASWBY010000764 DASWBY010000000
VERSION     DASWBY010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627548"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VF870_13845"
     CDS             <1..1192
                     /locus_tag="VF870_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9253324.1"
                     /translation="HRDLYRLPWTLPDNAISWLEPTAMCNLACDGCYRENQKDSHLSI
                     TEVKHHLDVFQKLRNTDCISIAGGDPLLYPDLLEIVADIKKRGLKPIINTNGKALTME
                     LLRDLKKAGVYGFTFHVDSKQGRGNEWKGKNEIELLDLRLQYAKMLAEVGGISCSFNS
                     TVYEDTLQYVPEMINWAHKHIDIVHTMVFIVFRHVIPNMPFDWYAGGTKVEWDKIMYH
                     SDKKRDVEILSTDVLAKVRERFPEFTPAAYLNGTVKADSFKWLLTERVGTKDKIYGYL
                     GPKFLELMMTVHHFFKGTYLSYASPSLTKKGRSTLLLLWPFDKRLKKAMFNFLKNPIN
                     LFKKTHLQTIMFIQPVDFTEKGEQNMCDGCPDITVHKDELIWSCRMEEQKQYNTWLRT
                     VPKN"
     gene            1386..2042
                     /locus_tag="VF870_13850"
     CDS             1386..2042
                     /locus_tag="VF870_13850"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX9253325.1"
                     /translation="MIKSLIKLLSDHSHITLIVSILLLILFSPYYWDAPLSNYFNFIF
                     LSFILISAILTLKKSSDMLKVVKRAGYLIVILTLITAITENSYLELVNRILFLLFFIL
                     VSINLLMGIIRSREVDADLIYSAVAVYLLFGFCGAVLAAVIMFFEPTAFSLNSTYSGQ
                     FHQFLYFSFITITSTGYGDILPITPLARTHAIFLALFGQLYLTVIIGILIGKYLSNKK
                     "
     gene            2187..4058
                     /locus_tag="VF870_13855"
     CDS             2187..4058
                     /locus_tag="VF870_13855"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX9253326.1"
                     /translation="MQKLIIILLLTTFIGNFSSYPDSDGDNETKNILILFSLIPTTPA
                     YRTILDGIRQKLTEEYGADYRLHQEYLETDRSTDESFQSERFKLYNEKYRNLNIDLLI
                     VVGVNIIPTVKKYASKYLLELPTVSLDYDFSNYGFQTDISLNNKTALLALKLNAEKSI
                     ATALDLFPNTETVYFICGSSIYDKLFLEISKKAAEKAIKDKNVIFITDIIMDDLLREV
                     KLLPVKSIIFIPNFIRDSKMIPYFNPETIRLVSRESNRPVFNYNDTGFGDGSIGGYVI
                     SFEKVGPLTGEVAVKILNGADPNSIKIDETDYYSYQFDWRELKKWNIETSEAIPEGSI
                     IKFEQVNFLNKNKWLIGAALLFMVLQSLLIANLIRLNRNQKLMTNKLIESEGKYREFL
                     HVDRSLRLGQLTASLSHELNQPFTAILSTAQAGINFINSNEATPELLKQLFQKIAEND
                     KRGASILSSIRGMMKLETRQKGKIDLNKLIEEIAAVFHSEASKLNIKLEVELTDKPAY
                     VLADGVQIQQVLLNFILNASQAMEKVNNSNKKIIISNTISSGDVIVAVQDSGKGIDES
                     EKEKLFKPFITSKKEGTGVGLVICRSIIEDHDGKIWAENIPDGGAKFSFSLRMLKDE"
BASE COUNT         1504 a          650 c          729 g         1383 t
ORIGIN      
        1 tcatagggat ttatacaggt taccctggac attacctgat aatgcaatat catggttaga
       61 accaacagca atgtgtaatt tagcttgcga cggctgttat agagagaatc aaaaagattc
      121 acatctatca atcacagaag tcaaacacca tcttgatgtt tttcaaaaat taagaaacac
      181 agattgtata tcgatagcgg gcggtgatcc tcttctttat ccggatctgc tcgaaatcgt
      241 agctgatatt aaaaagaggg gattaaaacc cataattaat actaacggta aagcccttac
      301 aatggaactg ctgagagatt taaagaaagc aggtgtttat ggttttacat ttcacgtaga
      361 tagcaaacag gggcggggaa atgaatggaa aggaaaaaat gaaattgaac ttcttgatct
      421 aagacttcaa tatgcaaaaa tgcttgcaga agtgggcgga atatcctgtt cattcaattc
      481 aactgtttat gaagatactc tgcagtatgt tcctgaaatg atcaactggg cgcataagca
      541 tatagatatt gttcatacga tggtcttcat agttttcagg cacgttattc caaatatgcc
      601 ttttgattgg tatgccggag gaactaaggt tgaatgggat aaaattatgt accattcaga
      661 taaaaaaaga gatgtagaaa ttttatcaac agatgtttta gcgaaagtaa gagagagatt
      721 tccggaattt actccggctg cctatttgaa cggcacagtt aaagcagatt cttttaaatg
      781 gttattaact gagcgggtag gtacgaagga taagatttac ggctatcttg gtcctaaatt
      841 tcttgagctt atgatgactg ttcatcattt ctttaaagga acttatttat cctatgcttc
      901 gccttcgctg acaaaaaaag gaagatcaac actgttactg ctttggccgt ttgacaagcg
      961 tttgaaaaaa gcaatgttta atttcctgaa gaaccctatt aatcttttca agaaaactca
     1021 tctgcaaacg attatgttca ttcagcctgt tgactttaca gaaaaaggcg aacagaatat
     1081 gtgcgatggc tgtccggata ttacagtcca taaagatgaa ttgatttggt cctgcagaat
     1141 ggaagaacaa aagcagtata atacctggtt aagaacggta ccaaagaatt aataattatt
     1201 ttagaaagaa ctatatatat tcatcttcga tgaatacaaa taggaattag attcttaagg
     1261 cacaattcat tagataattt agattaagct gtttacaata attcaggatt gtaaacagca
     1321 aaatcttaaa gttttaagag aattttaata ctggttttat tttttttgga attcaataga
     1381 gaaaaatgat taaatcatta attaaattac tttcagatca ttcacacatt acgctcatag
     1441 taagcattct gcttttaata cttttctcac catactactg ggatgcacca ttatcaaact
     1501 acttcaattt tatttttctc agctttatac ttatttcagc aattcttact cttaagaaaa
     1561 gcagtgatat gttaaaagtt gttaagcggg cgggttattt aatagtaatc ctaactttaa
     1621 ttaccgcaat aaccgaaaac tcctatcttg aactagttaa caggatttta tttttactat
     1681 tttttatact cgtatcgatt aatttattaa tgggaattat tagaagcaga gaagtggatg
     1741 ctgatttaat ttacagtgca gttgcggttt acttattatt tgggttttgt ggtgcagttc
     1801 tggctgctgt aattatgttt tttgaaccaa ctgctttttc attaaatagt acctactcag
     1861 gtcagtttca tcaatttctg tactttagtt ttataacaat cacctcaaca ggatacggtg
     1921 atattcttcc gataacacct ctcgcaagaa cacatgcaat ttttcttgct ctctttggcc
     1981 aactttacct gacagtaatt attggaattt taattggcaa atatttatcg aataaaaaat
     2041 aattgttccc agaagtagga aaataattaa tcataaaaga tgaaattttt cataattgaa
     2101 ttatttattt cacaatttta taataaaata cttattttta attctaatta gttagtaatt
     2161 gtgactatta aataatcttt ttaagtatgc agaaactaat tataatatta ttactaacta
     2221 ccttcatagg aaattttagt tcatatcctg attctgatgg cgataacgaa acaaaaaata
     2281 tcctaattct cttttctctt atacctacga ctcctgcata cagaactatt cttgatggaa
     2341 taagacaaaa gctaaccgaa gagtatgggg ccgattatcg tttgcatcaa gaatatttgg
     2401 aaactgatcg ttccactgat gagtcctttc aaagtgagag attcaaactt tacaatgaga
     2461 aatatagaaa cctaaatatt gatctcctta ttgtagttgg agtaaatatc atcccgactg
     2521 taaaaaaata tgcatcaaaa tatcttcttg aattaccgac agtttcactt gattatgatt
     2581 tttcaaatta tggttttcaa acagatatta gtcttaataa caaaactgct ttactagctt
     2641 taaaattaaa tgcagaaaaa tctattgcga ccgcactcga tttatttccc aatacggaaa
     2701 ctgtttactt catctgtgga tcatccatct atgataaatt attcttagaa atttctaaga
     2761 aggcagctga aaaagcaatt aaggataaaa atgtaatttt tattactgat atcattatgg
     2821 atgacttgtt gcgagaagtg aaattgctgc ctgtgaaaag tattatattt atcccgaatt
     2881 tcatacggga tagtaaaatg attccttact ttaatccgga aaccataaga cttgtcagtc
     2941 gggaatcaaa cagaccagta ttcaattata atgatactgg ttttggcgat ggttctatcg
     3001 gtggatatgt tataagtttt gaaaaagttg gaccacttac tggtgaagtt gcagttaaga
     3061 ttttaaatgg tgctgatcca aattcaataa aaattgacga aacagattat tattcctatc
     3121 agtttgattg gcgagaatta aaaaagtgga acatagaaac atcggaagca attcccgaag
     3181 gaagtataat taagtttgaa caagttaatt ttcttaataa aaataaatgg ctaataggtg
     3241 ctgcacttct ttttatggtt ttacaaagtc tattgatagc taacctgatc cgcttaaaca
     3301 gaaaccaaaa acttatgaca aataagttaa ttgaatcaga aggtaagtac agagaattcc
     3361 tgcacgttga cagaagcctt aggcttggtc aattaacagc atcactatca catgaactga
     3421 accaaccttt tactgcaatt ttaagtacag cacaggcagg aattaatttt atcaactcca
     3481 acgaggcgac tcccgagtta ttaaaacaac tatttcaaaa aatagcagag aatgataaaa
     3541 gaggtgcttc aatactcagc agtatccgcg gaatgatgaa acttgaaacc aggcaaaagg
     3601 gaaaaataga tcttaataaa ttaattgagg aaatagcagc agtctttcat agcgaagcaa
     3661 gtaagcttaa tataaaattg gaagttgaat tgactgataa accagcatat gttcttgctg
     3721 atggagttca gatacagcag gttttgctca attttatttt gaatgcctcg caagctatgg
     3781 aaaaagtgaa taattcaaat aaaaaaataa tcatttcgaa tacgattagt agcggtgatg
     3841 taatagttgc agtacaggat agtggtaaag gaatagacga atctgaaaaa gaaaaacttt
     3901 ttaaaccatt cattacttca aaaaaagaag gcacaggggt tggtttagtt atctgccgtt
     3961 caattattga agaccacgat ggaaaaatct gggctgagaa tatacctgac ggcggtgcaa
     4021 aattttcatt cagtttaaga atgttaaaag atgaataaga ttatccagta ataaattgaa
     4081 actcattttg attatttacc tcctaattgt tttaatttta aacaaaccga ttgtagaaga
     4141 acacaccact aaactactcg cttaaaggat acctagatgc ttagttaaaa gcattaaata
     4201 ggactattta cctatttgtt gctaatttaa attaaaataa ttttaatatt aaatatcttt
     4261 aaataa
//
LOCUS       DASWBY010000765         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_746201, whole genome shotgun sequence.
ACCESSION   DASWBY010000765 DASWBY010000000
VERSION     DASWBY010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746201"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VF870_13860"
     CDS             <1..920
                     /locus_tag="VF870_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9253327.1"
                     /translation="NVTGIEPDYYELSKMIHQYGGYSFIDFACAAPYVKIDMHPADPE
                     AKLDAIFFSPHKFLGGPGTPGVLVFDSKLYHNRVPDLPGGGTVDWTNPWGQHKFVSNI
                     EAREDGGTPAFLQTIKAALCIKLKEEMGVDNIRARENELVRIAFTGLKKIPTLHILAG
                     NIEHRLGAISFYIEDIHYNLIVKILNDRFGVQVRGGCSCAGTYGHYLLHVDPNRSKII
                     TDKIDQGDLSEKPGWVRMSIHPTMTDEELNIALDGIAQIVENVDGWSKDYIYDKSKNE
                     FFHKNSDGSDLEKIKSWFSLSQLQNVEID"
     gene            1035..1742
                     /locus_tag="VF870_13865"
     CDS             1035..1742
                     /locus_tag="VF870_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HEX9253328.1"
                     /translation="MLAIGAYEKCRQYINLHSSDSDTKKEIFPCITISRQTGSGSYEV
                     SEKLIYILQNKTKDPDNTWTYFNKDLITKVIEEFKLPKVFKDYITENKYNHISDAVNE
                     LLGVKPAEWTIVHKTTKIILQLARYGKTIIVGRGSNIITSKLPNSFHVRLIAPIENRL
                     KHVQEVFKYSKSEATNYIKKEDLNRHKYLKSHFFRDPDDPTLYHIIINTGKLTYEESA
                     AVIAEALIKKFPGSFID"
     gene            complement(1768..2634)
                     /locus_tag="VF870_13870"
     CDS             complement(1768..2634)
                     /locus_tag="VF870_13870"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9253329.1"
                     /translation="MSNIFHQIRYPFTTKFIKLNEFEIAYIDEGTSENILLFIHGLGS
                     YLKAWNRNIPELKKHFRCIALDLPGYGKSSKKIHSGSVSFYVKMLNEFILKLKLKNVS
                     LVGHSMGGQLASAYTIEYPDQINKLILVAPAGFETFNDQEIDLLKKIISPDILSRTSD
                     NQIRINYQINFFKMPVEAEEMIDDRIAIKNDEEFFNHCTNVSNSLFGLLYEPVFNRLK
                     EIHSPTLILFGKSDLLIPNKSIHQTRTEEVAHLGATQIKNSKVILLDECGHFLQYEKP
                     TEFNSQLISFFK"
BASE COUNT          974 a          461 c          498 g          921 t
ORIGIN      
        1 caaacgtaac tggcattgag ccggattact atgaattatc caaaatgatt catcaatatg
       61 gtgggtattc atttatagat tttgcctgtg ccgcacctta tgttaaaatt gatatgcatc
      121 ctgctgatcc cgaagcaaag cttgatgcaa tatttttttc tccacataaa tttttagggg
      181 gaccgggaac accgggagtt ttggttttcg attcaaaact ttatcacaac agagttccgg
      241 atttacccgg cggcggaact gtagattgga ctaatccttg gggacagcat aaatttgttt
      301 ccaatattga ggcccgtgaa gatggcggca ctccggcgtt tcttcaaaca attaaagcag
      361 cgttatgtat aaaattaaaa gaagaaatgg gcgttgataa tatcagagca agagaaaatg
      421 aactagtaag aattgctttt accggattaa aaaagattcc aactctgcat attctggcgg
      481 gtaatatcga acacaggctt ggagctattt cattttatat tgaagacatt cactacaatt
      541 taattgtaaa gatattaaat gatcgttttg gtgtgcaggt aagaggaggc tgttcctgtg
      601 ccggaactta cggacattac ttattgcacg ttgatccaaa tcgatccaaa attattacag
      661 ataaaattga ccaaggtgat ctatctgaaa aaccaggttg ggtaagaatg tcaattcatc
      721 caaccatgac agatgaagag ttaaatattg cactggacgg aattgcccag atagttgaaa
      781 acgttgatgg gtggagtaaa gattacattt atgataaatc caagaatgag ttttttcaca
      841 aaaattctga tggcagcgat ttagaaaaaa ttaaaagctg gttttcattg tctcagctcc
      901 aaaatgttga aattgattga ctttgattaa aaatctatta tactatctaa tgtaataatt
      961 ttattttaat gtgattaact gaatacacac gttttcctgt tttacaaaat aaaaattgga
     1021 aggagggtgt gattatgtta gcaataggtg cttacgaaaa atgtaggcaa tatattaatc
     1081 ttcattcatc agattctgat acaaagaaag aaatatttcc ttgtataacc atctcacgcc
     1141 agacaggttc cggttcttac gaagtctcag aaaaacttat ttacattctt cagaataaaa
     1201 ctaaagatcc cgataacacc tggacctatt ttaataagga cctaattact aaagtgattg
     1261 aagaatttaa actgcctaaa gtttttaaag attacattac tgaaaataaa tataatcaca
     1321 tctctgatgc tgttaatgag ctgcttggtg taaagccggc tgaatggacc attgtgcaca
     1381 agacaactaa aattattctt cagcttgcac gatatggaaa aactattata gtaggaaggg
     1441 gcagtaatat aattacttct aaattaccga atagttttca tgtacgattg attgccccaa
     1501 ttgaaaatag attaaaacat gttcaagagg tctttaaata ttctaaatct gaagcaacaa
     1561 attacattaa aaaggaagac ctgaacagac ataaatatct aaaatcccat ttcttccgtg
     1621 atccggatga tccaactctt tatcacatta taattaatac aggtaaactt acttatgaag
     1681 agagcgcagc agtaattgca gaagctctaa taaaaaaatt tcctggtagt tttattgatt
     1741 agaaaattgc tattaataag ataaaattta tttaaaaaaa gatatgagtt gagaattaaa
     1801 ttcagtaggt ttttcatatt gcaaaaaatg tccgcattca tccaatagaa ttactttgct
     1861 gttcttgatt tgtgtagcac ccagatgagc aacttcttct gttctagtct gatgtataga
     1921 tttattagga ataagcagat cgctctttcc gaataaaatc aaagtcgggc tgtgaatttc
     1981 ttttaatcga ttaaaaacag gttcatataa aagaccaaat aaggagtttg aaacatttgt
     2041 gcaatgattg aaaaactctt catcattctt gattgcaatt ctatcatcga tcatttcttc
     2101 ggcttcaacc ggcattttga aaaagttaat ctgataattt attctaatct gattatctga
     2161 agtcctgctg agaatatcag gtgaaataat ttttttaagc aaatcaattt cctgatcgtt
     2221 aaaagtctca aaacctgcag gggcaactaa aataagttta ttgatctgat ccggatattc
     2281 tatggtataa gctgatgcta actgtcctcc cattgaatga ccgactaatg agacattttt
     2341 tagtttaagt tttaatataa attcattcag catcttaaca taaaagctta cactgccgga
     2401 atgaatcttc ttgctcgatt ttccataacc cggtaaatct aaagcgatgc atctgaaatg
     2461 ttttttcaat tcgggaatat ttctgttcca ggcctttaaa taactgccga gtccatgaat
     2521 aaaaagcaga atgttttcag aagtgccttc atcgatataa gcaatttcaa attcatttaa
     2581 ttttatgaac tttgttgtga aaggatatct gatttgatgg aaaatattgc tcatattatt
     2641 ttggattggc tcttcagttt ttaaaccggg aaaaaataaa acagccgcag aataaatcta
     2701 cggctgtaga aataatgttt atttaagaaa atctaaaata gcctggttaa actcatcagg
     2761 cttttcgatt tgaatcatat gacccgcatt atctagctgc actagtttaa catccagcat
     2821 tgttttagca cctttctcaa agacgtctga tgta
//
LOCUS       DASWBY010000766         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_557304, whole genome shotgun sequence.
ACCESSION   DASWBY010000766 DASWBY010000000
VERSION     DASWBY010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557304"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VF870_13875"
     CDS             <1..418
                     /locus_tag="VF870_13875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9253330.1"
                     /translation="DILSNMGGEIFLNTKVTKVIGDESIEAIETNQENIIECDLLLIS
                     CKNKPNINLAKKFGLICDSDRGILVDEYFRTSEEGIFAIGECAAHFDFFKGDLYTALL
                     SSTVVMEGSIVGSNLYSSIYNRNRSLEYLYSASARI"
     gene            510..2543
                     /locus_tag="VF870_13880"
     CDS             510..2543
                     /locus_tag="VF870_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9253331.1"
                     /translation="MKISFKVRPKILIFIALVVSIVMILSAYFELRQNKEEVFHLLTE
                     TAHSLSETITTSSINALNSSNELENLIAERLLNNARMIRVLDSLNLLSHEKLIKIGKA
                     NNIYRINIFDKNGDRVLTNRVIETDDHVHGEEKVNRYKEVKPILTGKTSELIIGLKQA
                     MHVDEQRFAVAVARSGGRGAIVVNMNAEDFLSFRKRIGIGKIIQEIGDDSEIEYVALQ
                     DSIGIMAASSTVKELSSFSDDTFLLDAKLNDSTFTRVTNFEDREVFEAVKGLYYAGEF
                     IGVFRIGISLDEVRSIETRIIRRIITISIILAAISIILLSVLFSNQNLNLITQEYANY
                     KSYTNSFLENMKEAVLTVSSDMKITLFNISAEKLFSVSASDILDKEINNTGIKSLDII
                     GENIKVNNTYRNLELSFESGDSTKFVSVNIIPNYDLDKNLDNYTIIINDITELKNIEE
                     QTKRNEKLLAMGELASGVAHEIRNPINSIGMIGQRLLREFTPNADTKEYNTLTKLLSN
                     EVSRVNKIITQFLQYAKPLDLQIVKVEISKLMMDFYHLFIEQAEQRNIEFKIKGDKNI
                     YANIDPELFKQAIINFIQNAFDAVENNGKVLVEYFTRDGNLMVTIKDNGRGILEKDQS
                     KIFDLYYTTRKEGTGIGLSISQKIISQHNGVIDFTSSNKGTTFNIRIPYNEKL"
     gene            2530..>3115
                     /locus_tag="VF870_13885"
     CDS             2530..>3115
                     /locus_tag="VF870_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9253332.1"
                     /translation="MKNYNILIIDDEKSQRDILYGYLTKKGYKVFTAESGSQGIKIIN
                     ENLVDIVFSDFKMPEMTGLEILAKVSQINPEISFVIITAYGTVENAVNAMHLGAYDYI
                     SKPVNLDELDLLIARIIENKNLKSENELLKKQLYEKYKLSSFISQSSKMEEVLSVAVR
                     VADSKATILITGENGTGKEVLAKSIHFISPRKNNP"
BASE COUNT         1174 a          418 c          565 g          958 t
ORIGIN      
        1 agatatttta tcgaacatgg gtggcgaaat attcctgaat acaaaggtaa ctaaagtaat
       61 aggcgatgag tcaattgaag caatcgagac aaatcaggag aacattattg aatgcgattt
      121 actcttaatt tcgtgtaaaa ataaacccaa cattaacctt gctaaaaaat tcgggctaat
      181 ttgtgattct gacagaggca tacttgtaga tgaatatttt agaacctcag aggaaggaat
      241 atttgcaatt ggtgaatgtg ctgctcattt tgatttcttt aagggtgatt tgtatacagc
      301 attgctttca tctacagttg taatggaagg atcgatagtt gggtcaaatc tttattcatc
      361 aatctataac cgtaacagat ctcttgaata cctatattct gcatcagcta gaatttagga
      421 agtaaataaa gatttaaaga gtccaaggtt ttaatttatt taattattaa tactcttcca
      481 gatcttaaat caaataatta actttgagta tgaaaatatc atttaaagta agaccaaaga
      541 ttctgatttt tattgcttta gttgtaagta ttgtaatgat attatctgct tatttcgaac
      601 ttcgccagaa taaagaagaa gtattccact tattaaccga aactgcccat tctctttccg
      661 aaaccataac cacaagcagt attaatgctc taaactcgag taacgagttg gaaaatttaa
      721 tagctgaaag attattaaat aatgcaagaa tgataagggt attagacagc ctgaatttat
      781 taagccatga aaaactgata aaaatcggta aggctaataa tatttacaga ataaatatct
      841 ttgataaaaa tggtgataga gttttaacaa accgagtaat cgaaactgat gatcatgtgc
      901 atggtgagga aaaagttaat cgttataaag aagttaaacc aatcttaaca ggtaaaacta
      961 gtgagctgat tattggttta aagcaagcaa tgcatgtaga tgagcaaaga tttgctgtgg
     1021 cagttgctcg atcaggcgga agaggtgcca ttgttgttaa tatgaatgca gaggattttt
     1081 tatcattcag gaaaagaatt ggtatcggaa aaattattca agagataggc gatgattctg
     1141 aaatagaata cgtggcactt caggattcga taggtattat ggccgcaagc agtactgtaa
     1201 aagaactaag cagcttttca gatgatacgt ttctgttaga tgcaaagctg aatgattcaa
     1261 cgtttacacg agtgacaaac tttgaagaca gggaggtttt tgaagcagta aaggggcttt
     1321 attacgcagg cgaatttatc ggagtattta ggataggaat tagtcttgat gaagtaagaa
     1381 gtattgaaac aaggattatc agaaggatta ttaccatatc aattatactt gccgctataa
     1441 gcataattct gctaagtgtt ttattctcaa atcagaatct caatctaatt acacaagagt
     1501 atgctaatta taaatcttat acaaattcat ttctggaaaa tatgaaggaa gctgttttga
     1561 cggtttcaag cgatatgaaa attactttgt ttaacatatc agcagaaaaa cttttttcag
     1621 tttccgcatc agatatactt gataaagaaa ttaataatac tggtattaag tcgttagaca
     1681 taatcgggga aaacattaaa gttaataaca cttacagaaa tttggagtta tcatttgaat
     1741 ctggtgacag cacgaaattt gtatcagtaa acattattcc aaattacgac cttgataaaa
     1801 atcttgacaa ctatacaata ataatcaacg atattacaga gttaaaaaat attgaggaac
     1861 aaactaaacg taatgaaaaa ttattggcca tgggcgaact tgcatcgggt gttgcacatg
     1921 agattcgcaa tccaattaat tctataggta tgattgggca aaggttactt agagaattta
     1981 cacctaatgc tgataccaaa gagtataaca ctttgaccaa gctgctttca aatgaagtta
     2041 gcagagttaa caaaattatc acccagtttt tacaatatgc aaaacccttg gatttacaaa
     2101 tagtaaaagt tgagataagt aaattaatga tggacttcta tcaccttttt attgaacagg
     2161 cagaacaaag aaatattgaa tttaaaatca aaggggataa aaacatttat gcaaatattg
     2221 atcccgaatt atttaagcaa gccataatta attttattca gaatgcattt gatgcggtag
     2281 aaaataatgg aaaggttttg gttgagtatt ttacaaggga tggaaatctt atggtaacaa
     2341 ttaaagataa tggcaggggg attcttgaaa aagatcaaag taaaatattt gatctttatt
     2401 atactacaag aaaagaagga acaggaattg gattaagtat ttctcaaaaa ataatctcgc
     2461 agcataacgg cgtaatagat tttacatcta gcaataaagg aacaacattt aatatcagaa
     2521 taccatacaa tgaaaaatta taacatttta attatagatg atgaaaaatc acaaagagat
     2581 attctttatg gatatcttac taaaaaaggt tacaaggttt ttactgcaga atcaggcagc
     2641 cagggtatta aaataattaa tgaaaaccta gttgatattg ttttttcgga ttttaaaatg
     2701 cctgaaatga ccggcctgga aattttagca aaagtttctc agattaatcc ggaaataagt
     2761 ttcgttataa ttactgctta cggcactgtt gaaaatgcag ttaatgcaat gcacttaggg
     2821 gcatatgatt acatttctaa accagttaat ctggatgaac tggatttact gatagcaaga
     2881 ataatagaga acaaaaatct taaatcagaa aatgagttat taaaaaagca actttatgaa
     2941 aaatataaat tatcttcatt catttctcaa tcttctaaga tggaggaagt attaagtgtt
     3001 gcagtaagag tagctgatag taaggctaca attttaatta caggcgaaaa tggtaccgga
     3061 aaagaagtgc ttgcaaaatc aatccatttt attagcccgc gtaaaaataa tccat
//
LOCUS       DASWBY010000767         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_68735, whole genome shotgun sequence.
ACCESSION   DASWBY010000767 DASWBY010000000
VERSION     DASWBY010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68735"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..849)
                     /gene="uppP"
                     /locus_tag="VF870_13890"
     CDS             complement(1..849)
                     /gene="uppP"
                     /locus_tag="VF870_13890"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEX9253333.1"
                     /translation="MSLIEAIILGIVQGLTEFLPISSTGHLTLAGKFMGLISAENPEH
                     WTSFIAVIQLGTMISIFVYFWKDLWNILTEFLKNNLQKRVKFSEQSTNSKLGWMIILG
                     TIPIVVIGLLFKDMIEGALTKNLYVISGSLIALAIILAVAEKTAKFKKNIEDITLLDS
                     ILIGLAQAVALIPGSSRSGTTITGGLFLGLKRDVAARFSFLLSVPAVLASGMLQLKES
                     LAYINYDMAVSMIVATIVSGISGYLAIDFLLKFLKKKTTFVFIFYRIGLGVLILFLLY
                     SNIIKP"
     gene            complement(859..1491)
                     /locus_tag="VF870_13895"
     CDS             complement(859..1491)
                     /locus_tag="VF870_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253334.1"
                     /translation="MKKIVTTLFVIVISVFSFAQTPNEVRGSMGIDFVSVPDLKNYLE
                     ANYTDILSDFSSAVNFSGSYGRMVGENSQLELELGYILNSYNSTSSLGSYDLTYSILM
                     PSLLYNYVISGSGYNFKFGGGAGVRFLSIDESLPGAGSSDKYSATGYGFILRAVGNTA
                     IAQNVYAHIGADVRYDVLGEPDSSLGENTIGTVNFSTLSFGLRLGISYQF"
     gene            complement(1488..>1832)
                     /locus_tag="VF870_13900"
     CDS             complement(1488..>1832)
                     /locus_tag="VF870_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253335.1"
                     /translation="IIVLYAFGSYVGLLMLDMQNFQPITFGMGWIIMSISAIGVIIPT
                     PGSTGSYHTLAKSTLVLIFGFSETISVAYAFLTHIIGYILFIITALIMFFVVKKQKEN
                     LFEVVETEIREV"
BASE COUNT          646 a          366 c          265 g          555 t
ORIGIN      
        1 ctatggtttt attatgtttg aataaagtaa aaataaaatt aaaactccga gcccaattct
       61 gtaaaagata aaaacaaatg tcgttttctt ttttaaaaac ttcagtagaa aatcaattgc
      121 cagataaccg cttataccgg atacaattgt tgcaacaatc atactgacag ccatatcata
      181 atttatatat gccaaagatt cttttagctg aagcattccg cttgcaagaa cagccggaac
      241 acttaacaaa aatgaaaatc ttgctgcgac atctctcttt aatcctaaaa ataatccgcc
      301 ggttattgtt gtgcccgatc tagatgatcc aggaatcaac gcaaccgctt gtgctaaccc
      361 aattaaaatt gaatcaagca aagtaatatc ctcaatattt ttcttgaact ttgcggtttt
      421 ttctgcaaca gcaagaatga ttgcaagcgc aattaaacta ccgcttatta cataaagatt
      481 ttttgttaat gcaccttcaa tcatgtcttt aaataacagg ccaataacaa caattggaat
      541 cgtaccaagt ataatcatcc agccaagttt tgaatttgtt gactgctccg agaacttaac
      601 acgcttttgt aaattattct ttaggaattc tgttaagata ttccaaagat ctttccagaa
      661 ataaacaaaa atagaaatca tcgtaccaag ttggataaca gcaataaaag aagtccagtg
      721 ttccgggttc tctgctgaga ttaatcccat aaattttcca gcgagagtta aatgcccggt
      781 gctgcttatg ggaagaaact ctgtaagccc ctgaacaatt cctaaaatta ttgcttcaat
      841 aagactcaaa ttaactcctt aaaattgata tgatattcct agacgcaatc caaatgaaag
      901 tgtgctaaaa tttacggttc cgattgtgtt ttcaccaagg ctgctatcag gttcacctaa
      961 aacatcataa cgaacatcgg cacctatgtg agcatacaca ttttgagcaa ttgctgtatt
     1021 acctacagca cgcaaaataa atccgtaacc tgttgcggaa tatttatcag aacttcctgc
     1081 cccgggtaaa gattcatcaa tagataaaaa cctaacgcct gcaccaccgc caaacttaaa
     1141 attgtaaccg cttccactta ttacataatt ataaagtagt gatggcatta atatagaata
     1201 agtcaaatcg tagcttccta aactggaagt tgaattataa gaattaagaa tataaccaag
     1261 ttccagttcc aactgactat tctctccaac cattctacca tacgaaccag aaaaatttac
     1321 agcagagcta aaatcactaa gtatgtccgt ataattcgct tccagataat tttttagatc
     1381 tggtacactt acaaaatcaa ttcccataga gcctcttact tcgttcggag tttgagcaaa
     1441 tgagaaaact gaaatgacaa ttacaaacaa agtggttaca atttttttca tacttcccgt
     1501 atttctgttt ctacgacttc aaataaattt tctttttgtt ttttcacgac aaaaaacatt
     1561 atcaatgccg ttatgataaa taaaatatat ccgataatgt gcgttaaaaa tgcataagcc
     1621 acacttatgg tttcactaaa tccaaaaatt aaaactaaag ttgattttgc aagagtatga
     1681 tatgagcctg tgcttccggg tgtaggtatt atcaccccaa ttgcgcttat actcataatt
     1741 atccagccca ttccaaaagt gattggttga aagttctgca tatccagcat taacaatcct
     1801 acataagaac caaaagcata taaaactatt at
//
LOCUS       DASWBY010000768         5782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_400003, whole genome shotgun sequence.
ACCESSION   DASWBY010000768 DASWBY010000000
VERSION     DASWBY010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400003"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF870_13905"
     CDS             <1..237
                     /locus_tag="VF870_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX9253336.1"
                     /translation="DEVEDLVHFSKNDMQKMAADPGDLVYISDKRKWLGGLKSIHSRY
                     GEPHDEDGVVYISKEQLEHALFVEGKMLEAEKEM"
     gene            443..640
                     /locus_tag="VF870_13910"
                     /pseudo
     CDS             443..640
                     /locus_tag="VF870_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            810..1424
                     /locus_tag="VF870_13915"
     CDS             810..1424
                     /locus_tag="VF870_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253337.1"
                     /translation="MIIYRPVGLYELRLIYESRLMAFPPRLPEQPIFYPVLNIEYARQ
                     IAFNWNTKSEPYAGYVTEFEIDDKYVNQFERKIVGGKQHEELWIPAEKLDEFNRQIVG
                     KIKVVDAKFGKDFQGFIPSDFGLKDKNAVSQFVILANTFEYSGMDFYLETKVNHLAVF
                     LNYPFWLKHDFSQDGVEESLKYKTLDAIKEIWSQSHSETPLMAS"
     gene            1572..2243
                     /locus_tag="VF870_13920"
     CDS             1572..2243
                     /locus_tag="VF870_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9253338.1"
                     /translation="MDRSKLYDYSDGSRFLNRSAKEIFTEIEKKNVWHEQESVSGFGS
                     ALAQVKTVIEELPKVIKELNIKTIFDIPCGDFNWFKEINLSNNIYLGGDIVEDIVHRN
                     NQKYQSDNINFVQFNMLEDIQESMDLVFCRDCLVHLSIMDIWKALLNIQKSNSRYLMT
                     TTFTQEEKNNDIVTGGWRPLNLMKAPFNFPEPIRIINENCTEKDGVFKDKSLAVWEIK
                     DLVLI"
     gene            complement(2264..2512)
                     /locus_tag="VF870_13925"
     CDS             complement(2264..2512)
                     /locus_tag="VF870_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253339.1"
                     /translation="MDTILLVLALPKGIVSEPPPRTAILNLQQICLPAGVYNLQLPAI
                     SKILPANYLTFINHLEQNAERKMKLKNSIQLNHFGLWY"
     gene            2511..3347
                     /locus_tag="VF870_13930"
     CDS             2511..3347
                     /locus_tag="VF870_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9253340.1"
                     /translation="MQNRIDEAIQMHYTRADLGELILAALEKAGKDLNRLTPEDLAPI
                     DQFHIRGRTATLELARTAGLDAAKHILDVGSGVGGTSRCLAKEFGCRVTGIDFTDEYC
                     KAAAMLSVKTGLAHLVDYRQGDATNLPFDDQVFDVVWTEHVAMNIPDKTKLYKEMHRV
                     LKPGGTLAIYDVLAGQVSPVLFPVPWARTPDTSFLVSPNELRKLLEETEFTITDWSDT
                     TEAARAWFVNLAEKIHNEGFPSLGFHLLLGTDFKVMAQNQGQNLQEGRIVLAQIVAKK
                     QK"
     gene            3534..4403
                     /locus_tag="VF870_13935"
     CDS             3534..4403
                     /locus_tag="VF870_13935"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX9253341.1"
                     /translation="MVSSIQKEKANLFLRYHQDKEILVLLNSWDIGSSKLIEASGYKA
                     IATTSMGIAASLGYPDCEIIQLSEMINVITGIVNSVQAPVTVDIEAGYGNNLNEIIES
                     VKKIIATGVAGVNIEDSLNLNPVLIDEMEFCERVSAIRSLSDSLGFHLVINARTDSFY
                     VSSGSPKEKLSASIKRGNKYREAGADCIFVQPVSDKETIKTLVKEINAPINILANPTI
                     GAGVTPTISELQDLGVARVSLGSSLMKATLALIKKAADELTEKGTYNILLDTLTPLPY
                     AAEAYRMAIGSHK"
     gene            4817..>5782
                     /locus_tag="VF870_13940"
     CDS             4817..>5782
                     /locus_tag="VF870_13940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9253342.1"
                     /translation="MANSSKSNVIHLKDKVKQTIKSINDGKTLDKRNLLKLLQNISDL
                     NLDSKFETELEERERKYSTLVSNLPGFIYRCANDKNWTMHFISDGCKKITGYNPSDFI
                     NNKKVAFNNLIHPDYQKTIWQKWQKSLKDKTFFEFEYPIINKNGKLKWVWERGRGIYS
                     DKGKVLFLEGFIEDISEKRNAEESVKLKNIVFESSIAANSIANLDGKIIDVNSSFLAL
                     WGYNHKSEVLGKSLNGFLKSKKDISTILAALNKIGKWEGDYVAKRKDKSEFIAFGLAT
                     TIVDDSGEVIGYQSSVIDITENRKTEELLKESESRYKSFFDNSPDA"
BASE COUNT         1934 a          979 c         1154 g         1715 t
ORIGIN      
        1 gacgaagttg aagaccttgt acatttttca aaaaatgata tgcaaaaaat ggctgctgat
       61 cccggtgatc ttgtttacat ttctgataaa cgcaaatggt tgggcggact aaaatccatc
      121 cactcccgct acggcgaacc acacgacgaa gatggtgttg tttacataag caaagaacaa
      181 cttgagcatg ccttgtttgt tgaaggcaaa atgcttgaag ctgaaaaaga aatgtaagac
      241 aaattagaat ttagaatcat taatttttga tttgtaaact gctgaagcaa aattataaaa
      301 attataattt tctattgacg atcaccattt aatttcttag gtttgaggaa aaatttatgg
      361 gagtcggttt ttgataggcg tcattaaaaa tatttccatt aaagcaattc ccctttggga
      421 atctaacaac aaaccccaac ttctagatca attccattca cacctaacta aaccagcaag
      481 cccacacact ttcaggcatt catttgcaac acatttgctg gaaaacagct acgatattag
      541 aactatacag gaattgctgg gacatgattc cgtaaaaact actatgatat acacacatgt
      601 tttaaataga ggattaggag ttaagagtcc gttagactga aaattgttag gatgatatat
      661 atgcatgaca tatttttaaa tattgcaatt ttagaaaatt ttggaatcaa ttggtatgta
      721 gatatgcaac tgcacgcata tctaagtact ttaactaata tttctgtgca gtataagtaa
      781 tagttagctc tccccttgcg caataaaaaa tgattatcta tcgcccagtt ggcttatatg
      841 aattacgttt gatttacgaa agcagattaa tggcttttcc tcctcgctta cccgaacaac
      901 caatttttta tcccgttctc aatatcgaat atgctcgaca aattgcgttt aattggaaca
      961 caaaaagtga accatacgcg ggttacgtca cagagtttga aattgacgac aaatatgtca
     1021 accagtttga aagaaaaatt gttggcggaa aacagcatga agaattatgg atacccgccg
     1081 aaaaactgga tgagttcaat cggcagattg tcggtaaaat aaaagttgtt gatgcaaaat
     1141 ttggtaaaga ttttcaagga tttattccaa gtgattttgg cttaaaagat aaaaacgccg
     1201 tttcacagtt tgtcatactt gctaatacat ttgagtacag tggaatggac ttttatcttg
     1261 agaccaaagt aaatcacctg gcggtttttc taaattaccc attctggttg aaacatgatt
     1321 tcagccaaga tggtgttgaa gaaagtttga agtataagac gttagatgcc atcaaagaga
     1381 tatggtcaca atcgcattca gaaactcctt tgatggcttc ttaaaagtct aaagcgtgca
     1441 ctcgacaagt gggattctgc gccatttaaa agcctttttc tcacttcggg tttatactcg
     1501 cctcaagccg agtccacgcc cgcccacttg cgggtaacgc aaaccgttat accaaaaatt
     1561 gtgagtcaca tatggatcgt tcaaaactat atgattattc agatggcagt agatttctaa
     1621 atagatctgc aaaagaaata tttacagaga ttgaaaagaa aaatgtctgg cacgaacaag
     1681 aatcagtttc cggatttgga tcggcattag cacaagtaaa aactgttatt gaagaattac
     1741 cgaaggtaat aaaagaattg aatattaaaa ctatttttga catcccttgt ggagacttta
     1801 attggtttaa agaaattaat ctctccaata atatttattt gggtggtgat atagtagaag
     1861 acattgttca cagaaataat cagaaatacc aaagcgataa tataaatttt gttcaattca
     1921 atatgcttga agatattcaa gaatcaatgg acttggtttt ttgtcgtgat tgcctggtac
     1981 acttatcaat aatggatatt tggaaagcct tattaaacat ccaaaaaagt aattcaagat
     2041 acttaatgac aactacgttt actcaggaag aaaaaaataa tgacatagta acgggtggtt
     2101 ggcgtccatt gaatttaatg aaagctccat tcaattttcc agaacctatc cggattatta
     2161 atgaaaattg tacagaaaaa gatggcgttt tcaaagataa gtcattagct gtttgggaaa
     2221 taaaggattt ggtactaata taaattttgg cataaccagc ttatcaatac caaaggccaa
     2281 aatggtttag ttgaatgctg tttttaagtt tcatttttcg ttcggcattt tgttctaagt
     2341 ggttaataaa tgtaagatag ttcgccggca gtattttgga aattgctggc agctgcaaat
     2401 tataaacacc tgccggcagg cagatttgtt gcaagttaag aatcgctgtt ctgggcggcg
     2461 gctcagacac aatgccttta ggcagtgcta ataccaggag aatcgtgtcc atgcaaaatc
     2521 gtatcgatga agcaatccag atgcactaca ctcgcgcaga tctgggagaa ttaatactgg
     2581 cggcgctgga gaaagcgggc aaggacctta accgactgac gcccgaagac ttagcaccta
     2641 ttgaccagtt tcatatccgc ggacggacgg cgacgctgga gcttgcccgg actgcagggc
     2701 tggatgcggc aaaacacata ctcgatgttg gtagtggtgt aggcgggacc tcgcgttgtc
     2761 ttgcgaagga attcggatgc cgcgtcaccg gtattgattt cacagacgag tactgcaaag
     2821 ctgctgcgat gctttctgtc aagaccggcc tcgcacacct cgttgactat cgtcagggtg
     2881 acgccaccaa cctgccattt gatgaccagg tattcgatgt cgtgtggacg gagcatgttg
     2941 cgatgaatat cccggacaag actaaactgt acaaggaaat gcaccgggtc ctgaagccgg
     3001 gaggcacgct tgctatatac gatgtacttg ccggtcaggt aagcccggtt ctgtttcctg
     3061 tcccgtgggc gcgcacacct gatacaagtt tcctcgtatc gccaaatgag ttgcgcaagc
     3121 ttctcgaaga aacagaattc acgattactg actggtctga tacaacagag gcggcacgtg
     3181 cgtggtttgt taacctggcc gaaaagatcc acaatgaagg ctttccatct cttggattcc
     3241 atttgctgct tggaactgat ttcaaagtga tggcgcaaaa tcaggggcaa aacctgcaag
     3301 agggacggat tgttcttgca caaatcgttg cgaagaaaca gaagtagcct aacaatgttg
     3361 cacaaccaca agcaccagtg ggtgcgacta ataccgaatg ttaaaaaatt attatcaatt
     3421 tatgtataat caaaatacaa ttgattaata attgttcttt ttatatttat gttgtggtta
     3481 actcaatgat atgtttgatg tattaatatt gaatattaaa aaaggacgct atcatggttt
     3541 caagcattca aaaagaaaaa gcaaatctct tcctgagata tcatcaggat aaagaaattc
     3601 tggttctgtt aaactcgtgg gatatcggaa gttctaaatt aatagaagca agcggctata
     3661 aagccatcgc aactactagt atgggaatag ctgcttcttt aggttatcct gattgcgaga
     3721 taatacaact ttcagaaatg attaatgtta taaccggtat tgtaaattca gtacaggcac
     3781 cggtaactgt ggatatagaa gccggatatg gaaataattt aaatgagata atcgaatctg
     3841 ttaaaaagat aattgcaact ggagttgctg gagtaaacat tgaagatagt ctgaacttaa
     3901 atccggtctt aattgacgaa atggaatttt gtgaaagagt atcagccatt cgttcattat
     3961 cagattcact tggctttcat ttagtaataa atgcaagaac tgattcgttt tatgtttcat
     4021 caggttcgcc aaaagaaaaa ttatcagcat caataaagcg cggcaataag taccgggaag
     4081 ccggggcaga ttgcatattt gtacagcctg tatcggataa agaaacaatt aaaacactgg
     4141 taaaggaaat caatgctccg attaatattc ttgcaaaccc tactattggg gctggagtaa
     4201 ctccaaccat aagtgaactg caggatttag gcgttgcccg tgtaagtctt ggttcaagtt
     4261 tgatgaaagc tactttagca ttaatcaaaa aagctgccga cgaactcact gaaaaaggga
     4321 cttacaatat tttactcgat actctgacac cgcttcccta tgctgcagag gcatatagaa
     4381 tggcaatagg atctcataaa tgaactgatc atatctagga agttgagaga tgttaaaaac
     4441 aataaattta atagttttat gattcgtttt tgaaaccgaa atcggcttag tatttaacca
     4501 gtttaatttt taatgagagt tttttcattt cgggtttctt ttaaaacagg tttaaagcga
     4561 aagattttcc tttacaaaaa ggttattttt tatatttgat attaggcgtc ggtgtatgct
     4621 gaactaaaaa tatttatata gtatttcgct aatttagaac acttcccttc tgataaacca
     4681 attagttatt atttaatgta aatcatttaa aatttttctt tacatttctt gtatcaatta
     4741 caatatttgg agaaattaga attgtttttg ctgcatttta attgttcaaa taaaatgtgt
     4801 tataaaggag ttttggttgg caaatagtag taaaagtaac gtcatccatc taaaggataa
     4861 agttaagcag acaattaaaa gtattaacga cggtaagact ttagacaagc gtaatttgct
     4921 caaacttctg cagaacattt ctgacttaaa tctcgattcc aaatttgaaa ccgaacttga
     4981 ggaaagagaa cgtaagtatt ccacccttgt aagcaaccta cctggattta tttacagatg
     5041 cgccaatgat aaaaattgga ctatgcattt tataagcgat ggctgcaaaa aaattacagg
     5101 ttataaccca tccgatttta ttaataataa gaaagtagct ttcaataatt taatccatcc
     5161 ggattaccaa aaaacgattt ggcaaaagtg gcaaaaaagt ttaaaggata aaacattttt
     5221 tgaattcgaa tatccaatta ttaacaaaaa cggaaaactt aaatgggttt gggaacgcgg
     5281 cagaggtata tactctgata aaggtaaggt acttttttta gaaggtttta ttgaagatat
     5341 aagcgaaaaa agaaatgctg aagaatctgt taaacttaaa aatattgtgt tcgaatcttc
     5401 tatagctgcc aacagcattg ccaatcttga tggaaaaata attgatgtca attcgtcatt
     5461 tttagcactt tggggttaca accataagtc tgaggtattg ggtaaatcac taaacggatt
     5521 tctaaaaagt aaaaaagaca tttcgactat ccttgccgca ttaaataaaa taggcaaatg
     5581 ggaaggcgat tacgttgcaa agcggaaaga taaatcagaa tttattgcat ttggtctggc
     5641 aactacgatt gttgatgact cgggagaagt aattggttat caatcctccg taatagatat
     5701 tactgagaac cgcaagacgg aagaattatt aaaggaaagc gagagtagat ataaatcgtt
     5761 ttttgataat tcacctgatg ca
//
LOCUS       DASWBY010000769         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_520010, whole genome shotgun sequence.
ACCESSION   DASWBY010000769 DASWBY010000000
VERSION     DASWBY010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520010"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VF870_13945"
     CDS             complement(<1..302)
                     /locus_tag="VF870_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253343.1"
                     /translation="MKKATFFFLSWFAALSVFTFAQVQLKFVTINGYGNNLYIDNVTV
                     GNQFNLDAAVGSINNINSGTSYATFTSPFVIVPNVSVINIGKQNITTSFTVTMTATP"
     gene            complement(361..1170)
                     /locus_tag="VF870_13950"
     CDS             complement(361..1170)
                     /locus_tag="VF870_13950"
                     /inference="COORDINATES: protein motif:HMM:NF022985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HEX9253344.1"
                     /translation="MKIFLLLIIPLMFLGCETNPPTQPISLKDGEVKINITISEDNSI
                     SSDRTVLLEDFANVSCTPCVTSNKIIQSVINSYGSSKIVSVKFPTNFPSPVDPFYLAA
                     KEFCDFRMTYYNILFAPTIILDGITLPTPTDSNAIKQSINNRLTIPSNFNVTVAKTVV
                     NGGLLLNVDIKLKNKNSLDLDDLFLRCAIVESEVEYEQPPGSNGETKFYDVLRVLLPS
                     NEGISLSSVGSALTYQFENTIDTVWNIDKLEAVMFIQSSANKEIQQTGSSF"
     gene            complement(1351..1710)
                     /locus_tag="VF870_13955"
     CDS             complement(1351..1710)
                     /locus_tag="VF870_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type lectin domain-containing protein"
                     /protein_id="HEX9253345.1"
                     /translation="MKNICISILLFSFIFLTGFSSAQTMQSSSWSVNQSLAGYSLDKN
                     DGERTMTIDIDFENSFTEKPQIILSVTQIDSDKETNLRYNVEAISISRDGFTIKVRTW
                     ADSKLFSISGYWLATTK"
     gene            complement(1806..2156)
                     /locus_tag="VF870_13960"
     CDS             complement(1806..2156)
                     /locus_tag="VF870_13960"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX9253346.1"
                     /translation="MNIETIIEYCLSKKGAIEDFPFDEETLVFKVGGKMFALIPLEKI
                     PLQINLKCDPEKAIELREQYEDVQPGWHMSKKYWNTVILDGNIRWAELKDWIDHSYDE
                     VVKGLKKSERDKIK"
     gene            2226..>2470
                     /locus_tag="VF870_13965"
     CDS             2226..>2470
                     /locus_tag="VF870_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX9253347.1"
                     /translation="MFVYFNDKYIPHEEVKLSPFDRAFLFADGVYEALRTYNGKLFRL
                     DDHLERLRYSLTQLEIPFNDFSLIEEITSKLAQINNIT"
BASE COUNT          808 a          408 c          376 g          878 t
ORIGIN      
        1 ggtgtagcgg tcattgtaac tgtaaaggag gttgtaatat tttgttttcc tatgtttata
       61 actgatacat taggaacaat aacaaacggt gatgtaaatg tagcatagct cgtgccagag
      121 ttaatattat tgatagagcc aactgctgca tccaaattaa actgattacc aacagttaca
      181 ttatctatgt agagattgtt tccgtaacca ttaatagtca caaactttaa ttgtacctgt
      241 gcaaaagtaa aaacacttag tgcagcaaac cacgaaagga aaaaaaatgt agcttttttc
      301 atatgaacct ctcctgtaaa atgaatttga tagttaacta gatacaaaga acttaattgc
      361 ttaaaaactc gatcccgttt gctgtatctc tttgttagca gaactttgta taaacatcac
      421 tgcttcaagt ttatctatat tccaaactgt gtcaatagta ttttcaaatt ggtaagtcag
      481 cgcacttcct acggatgata aagaaattcc ttcgtttgat ggtaatagca ctctgagcac
      541 atcataaaat tttgtctcgc cattggagcc tggcggctgt tcatattcta cttctgattc
      601 aacaattgca catcttaaaa ataaatcatc aaggtctaag ctgtttttat tttttaattt
      661 aatatcaaca ttaagaagca aacctccgtt aacaactgtt tttgcaactg tgacgttaaa
      721 attagaggga atggtcaatc tattatttat tgattgctta attgcattag aatctgttgg
      781 ggttggcagt gtaataccat ctaaaataat tgttggagca aataatatat tataataagt
      841 cattctaaaa tcacaaaact ctttagcagc taaataaaat ggatcaacag gtgaaggaaa
      901 gtttgttggg aatttaaccg aaacaatttt actgctgcca taactattta taacagactg
      961 gataatttta tttgaggtta cacacggcgt acaacttaca tttgcaaaat cttcaagaag
     1021 cactgttctg tcagaactaa tagaattgtc ttcagagatg gtaatattga tttttacttc
     1081 gccgtctttt aatgaaattg gctgtgttgg cggattggtt tcacatccca aaaacattag
     1141 cgggataatt aataaaagaa atattttcat acacactaaa ttttcaattg agatctactg
     1201 agcttatatc cccaataggg aaataataat ttttctgcgg ccaaaatata acgtcttaac
     1261 tgaaattcaa agaggaatta atttttattg taaaatttta attagaggct ggtttaattg
     1321 agaggtatga atatcttttt acttatcaat ttatttagta gttgccaacc agtaaccact
     1381 aatcgaaaat aattttgaat ctgcccaggt acgaaccttt attgtaaagc catctcttga
     1441 aattgatatg gcctcaacat tatatcttaa attggtttct ttatctgaat ctatttgagt
     1501 aacacttaat attatttgag gtttttctgt aaatgaattt tcaaaatcaa tatcgattgt
     1561 catggttcgc tcgccatcat ttttatcaag cgagtatcct gcaaggcttt ggtttacaga
     1621 ccaactgctg ctctgcatag tttgagcgga tgaaaatcct gtaaggaaaa taaaggagaa
     1681 gagtaatata gatatgcaaa tatttttcat agctgtttat tttttattca ctgttaaaaa
     1741 ttaatcgata tgttttcaat ttacaatcat aaatgctgct gccgtgatta taatcaatct
     1801 tataactatt tgattttatc ccgctcactc ttcttcaatc ccttaacaac ttcatcataa
     1861 gagtgatcaa tccaatcctt taattccgcc cagcgaatgt taccgtccag aataactgta
     1921 ttccaatatt ttttactcat atgccagccg ggctgcacat cttcatattg ttctcttaat
     1981 tcaattgctt tttccggatc acattttaaa tttatttgta atggtatttt ttctaaggga
     2041 attaacgcaa acatttttcc accgacttta aatacaagtg tttcttcatc aaaaggaaaa
     2101 tcttcaattg cgcccttctt acttaagcaa tactcaataa ttgtttcgat attcattctg
     2161 gtgcgttttc aatcgttaat aaaatttttt ttgattattt tatcatatca aaatattaca
     2221 caaaaatgtt tgtctatttc aatgataaat atattccgca cgaagaagta aagctctccc
     2281 cttttgatcg tgcatttctt tttgcagatg gagtttatga agctttaaga acttataatg
     2341 ggaaactctt tcgtttagat gatcatctgg aaagattgcg atatagttta actcaacttg
     2401 aaattccatt taatgatttt agtctaattg aagagattac tagcaaacta gcacaaatta
     2461 ataatatcac
//
LOCUS       DASWBY010000770         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_712043, whole genome shotgun sequence.
ACCESSION   DASWBY010000770 DASWBY010000000
VERSION     DASWBY010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712043"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VF870_13970"
     CDS             complement(<1..305)
                     /locus_tag="VF870_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253348.1"
                     /translation="MNKSFIQSAKNAIRFLTLTAVIESITGLLLVLLPSKFVSFLFGS
                     ALDSPVALIVAHIAGVALMALGITCWIARNDNQSSAVKGVIIGLTVYNAGVFALLAYA
                     "
     gene            complement(475..837)
                     /locus_tag="VF870_13975"
     CDS             complement(475..837)
                     /locus_tag="VF870_13975"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX9253349.1"
                     /translation="MISCDSKGNQLYGGKKYKLHLPPEIPASDFWSIVVYDLEDHLLI
                     NTDHQWPSVHSGNKKLIYNSDGSVDAWFGPDCIEGKESNWVKTIPGCQWYMILRLYNP
                     LETWFSKKWQHGEIEEVI"
     gene            1039..>1517
                     /locus_tag="VF870_13980"
     CDS             1039..>1517
                     /locus_tag="VF870_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253350.1"
                     /translation="MNKEDDKYSLFFEFIETYLHVGFKGIDRSDPLILSLEEMMKNTN
                     QFFYVFDMINMKVEFTSQRSYQMFSIKPEDLTPYHFKEAVHPDDLKRHGLGLAKVFKI
                     AHELFVAKKGEMLLSSNFRFRNITGSYTEQLVQCYLFFSPVPNNTVYIFSLKTDISW"
BASE COUNT          456 a          289 c          273 g          499 t
ORIGIN      
        1 gcataggcca ataaagcaaa aacacctgca ttataaacgg tcaatccaat gattacacct
       61 ttcacagcgc tgctctggtt gtcgttacgt gcgatccagc aggtaatacc caatgccatt
      121 aatgccactc ctgcaatgtg ggcgacaatc aacgcaaccg ggctgtcgag cgcagaccca
      181 aataaaaagg aaacaaattt tgatggaagc aaaaccagta gcaggccggt tatagattca
      241 atgactgcgg ttaatgttaa gaagcgaatt gcattttttg ctgattgaat aaatgactta
      301 ttcataatgc ttaatttttg ttaattttta ttaattgttt cgtttaaaaa tttaatccaa
      361 acctggttac cgtatctacc aaattaattt cttcatattc aatctggttt tgtaattttc
      421 tgtaagtaaa accggaattc cccggtgctt ggttctactt ttattattag tcatttatat
      481 tacttcctca atctctccgt gctgccattt tttactgaac cacgtttcga gaggattata
      541 caggcgaagg atcatatacc actggcagcc tggtatagtt ttcacccagt tgctttcctt
      601 tccttcgatg cagtcgggac cgaaccaggc atcaaccgaa ccatcactat tgtagataag
      661 ttttttgttg ccgctgtgca cggaaggcca ttgatgatcg gtattaatta aaagatgatc
      721 ctcgagatca taaactacaa ttgaccagaa gtctgaagcc gggatctccg gcggcagatg
      781 caacttgtat tttttccccc cgtaaagctg gttacctttt gagtcgcatg atatcatttt
      841 ttgcaattct tccttcattc cttttaacca ataaaccttt tactttttta ataatatcgc
      901 cggttctggt tttacccgat taggagatag taaaattatg tctatatacc acttgtaaca
      961 atacattaaa tagtgtatat tttatttata ctattatatt tgttgtattc atttattgaa
     1021 accggatcag tattatttat gaataaggaa gacgataaat acagcttgtt tttcgaattc
     1081 attgagacct atttacatgt tggttttaaa ggaattgaca ggagtgatcc tttaatactg
     1141 tcacttgagg aaatgatgaa aaacaccaat cagtttttct atgttttcga catgattaac
     1201 atgaaggtcg aatttaccag tcagagaagc tatcaaatgt tcagcataaa accggaggat
     1261 ctgactccat accattttaa ggaagcagtt catcccgatg atcttaaacg gcatggactg
     1321 ggattagcta aagtatttaa aatagcacat gaactttttg tggcaaaaaa gggagaaatg
     1381 ttattatcct caaatttcag gtttcgaaat atcacaggta gttacacgga gcaacttgta
     1441 cagtgctatt tgttttttag cccagtccca aataatacag tctacatatt tagtctcaag
     1501 actgatatca gctggtg
//
LOCUS       DASWBY010000771         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_746347, whole genome shotgun sequence.
ACCESSION   DASWBY010000771 DASWBY010000000
VERSION     DASWBY010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746347"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VF870_13985"
     CDS             complement(<1..382)
                     /locus_tag="VF870_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253351.1"
                     /translation="MASIRKINNHYHIDYYDEAGKRHRVALGIKASRENYKLAVIELK
                     KIEYELSSGIHLEKLTRIEKWNKTLIEGYDEFIKTKFSKSEKTKEHLEDSYQKFLIHI
                     GDMRIKNITARHFDELEIELKNKKK"
     gene            complement(357..692)
                     /locus_tag="VF870_13990"
     CDS             complement(357..692)
                     /locus_tag="VF870_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253352.1"
                     /translation="MLIDVEDLRAAILEKFGTQHNFAKKTGYAEPQITRAMQTQSPRF
                     MLACKRAGIDTAGLLMRQREKEELKKLKVNDPQRRIMELEQLVKDQKEIIDLMKKVLE
                     RVGIDKKNK"
     gene            complement(861..1031)
                     /locus_tag="VF870_13995"
     CDS             complement(861..1031)
                     /locus_tag="VF870_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253353.1"
                     /translation="MWSILVVLEDLGGFFFIVKVLSIVCMIDYKAFQGRMGNAYMLSE
                     RRKKEVKFSGIN"
     gene            1154..1558
                     /locus_tag="VF870_14000"
     CDS             1154..1558
                     /locus_tag="VF870_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253354.1"
                     /translation="MKKNINYYQHFADSHNHWKFKLLRSKLGWAGEGRFWALNNMIAA
                     SDGCTIDLNRKNLKASVMFDLGLSANEFDEFINILSNECELILNLDGIITTEIVRDNL
                     REVMKQRERVRLNKKHDKTQSDNKTQNLRILK"
     gene            1700..2011
                     /locus_tag="VF870_14005"
     CDS             1700..2011
                     /locus_tag="VF870_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253355.1"
                     /translation="MKTLISKEIARLRKFGFTVYNFNLNKSLPAGLKNFVDHLIISER
                     YLIFVEVKIGKDKLNLEQIDLMNLIYFLGIRNKSIHYQLLNNVKQTKDFVSAVLSNKL
                     K"
     gene            2030..2389
                     /locus_tag="VF870_14010"
     CDS             2030..2389
                     /locus_tag="VF870_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253356.1"
                     /translation="MTKLENFYSLLQFLNYGKANAKTADQLSVYFEHKSLSDFKRKLR
                     LIAQEARLNGHWVIGDDSGYYMALTKEEWHNYRNKRFHAINTELQALASCDKISIKDL
                     IKNVYAVSVDDQNYNLF"
     gene            2441..2623
                     /locus_tag="VF870_14015"
     CDS             2441..2623
                     /locus_tag="VF870_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253357.1"
                     /translation="MNEFDAANQYLLNHYEQLAAAVELFLESPSDCKSAYLEMMQKSL
                     SDLKEFKSGLSPLDEP"
BASE COUNT          871 a          490 c          401 g          876 t
ORIGIN      
        1 gctttttttt attttttaat tctatttcca gttcatcaaa atgtcttgct gtaatattct
       61 taattctcat atccccgata tgaatcagaa atttttgata tgaatcttcc aggtgttctt
      121 ttgttttttc actcttgctg aatttagtct ttataaattc atcgtatcct tcgattaatg
      181 ttttattcca cttttcaatt cttgtaagtt tctcaagatg tatccccgaa ctcagctcat
      241 attcaatttt cttcaactcg ataacagcaa gcttgtagtt ttccctgctc gctttaatcc
      301 ctaaagcaac cctgtgcctc ttgcctgctt catcataata atcaatatga tagtgattat
      361 ttatttttct tatcgatgcc aactctctct aacaccttct tcattaaatc gattatttct
      421 ttctgatctt ttactaactg ctctaactcc ataatacggc gctgaggatc attgactttt
      481 aattttttga gctcctcctt ttctctttgc ctcatcaaca atccggcggt atcaatgcct
      541 gctcttttgc aagcgagcat aaatctaggt gactgcgtct gcattgcacg cgttatctgc
      601 ggctctgcgt atccggtctt cttagcaaaa ttatgctgtg tgccgaactt ctctaagatc
      661 gcagctctta aatcttctac gtctattaac attttttaac actttcttga tatttattga
      721 caatttaatt aatattaaaa cagacatttt aacattttca cattttaatt tatttatttt
      781 atttgccttt gtcaactgaa acatactcta taagtgaagc ggccaagctc ttaaaagtcc
      841 gccagtctta tatcagaatg ctaattgata ccggaaaact tgacttcttt cttcctccgt
      901 tcagaaagca tatacgcatt ccccattctt ccctggaaag ctttataatc aatcatacat
      961 acaatagaaa ggaccttaac aatgaaaaaa aatccgccta aatcttctaa aacgactaaa
     1021 atgctccaca tagatgctgc tgttcatcta aaattcaaac gcttctgctt aagcaataac
     1081 cagactatgc agtctcttgc aaacaaagct cttaaggaat ttatgaaagc accggaaatc
     1141 atctgatttt ttaatgaaaa agaatattaa ctattatcaa cattttgcgg actcccataa
     1201 tcactggaaa ttcaagcttc tccgctctaa gctcggctgg gcaggtgaag gaagattctg
     1261 ggccctgaat aatatgatag cggcctctga cggctgtact atcgatctta acaggaaaaa
     1321 cctcaaggct tctgtgatgt ttgatcttgg tttgtctgca aatgaatttg atgaattcat
     1381 taatattctt tcaaatgaat gcgaactcat tcttaatctc gatggaatta tcacgacaga
     1441 aattgtaaga gacaacctca gagaggttat gaaacagcgc gaaagagtcc gtcttaataa
     1501 aaaacacgat aagactcagt ctgataataa aacacagaac cttcgaattc ttaaataatt
     1561 ttttgagttt agctaaactt ctcaaaagtt tagataaatt tttcacaaag aaagaaagaa
     1621 agaaagaaag aaataggatc ttcaatcaaa tgtggataaa aatgtggata actcttgatc
     1681 cgttagaaag aaaattttta tgaaaacatt aatcagtaaa gaaattgcgc ggctcagaaa
     1741 gttcggattt actgtctaca attttaatct taacaaaagt ttgccggcag gattaaaaaa
     1801 ttttgtagat catttaatca tttccgaaag atatctgata ttcgttgaag tcaagatcgg
     1861 caaggataaa cttaatctag agcagataga tcttatgaac ttaatttatt tccttggcat
     1921 cagaaataaa tcaattcatt atcagctgct taacaatgta aaacagacaa aagattttgt
     1981 ctcagcagtg ttatcaaata aactaaagta atttttttta gaggtaaata tgacaaagct
     2041 ggaaaatttt tacagccttc ttcaatttct taattacgga aaagcaaatg caaaaacagc
     2101 tgatcagtta tcagtttatt ttgagcacaa aagtctgtcc gatttcaaaa gaaaattacg
     2161 cttaatcgct caggaagcac gattgaatgg acactgggta atcggtgatg acagcggata
     2221 ttatatggct ctcacaaaag aagaatggca taattataga aacaaaagat ttcacgcgat
     2281 caatactgaa ctgcaggctc ttgccagctg tgacaaaata tcaatcaaag atttaatcaa
     2341 aaatgtatat gcagtttcag ttgatgatca gaattataac ttattttaaa ataatttttt
     2401 taattaaatc attcacaaca aaataggagg taaaataatt atgaatgaat ttgatgcagc
     2461 aaatcaatat ctgctgaacc actatgaaca gctcgcagcc gctgttgaat tatttctgga
     2521 atccccttca gattgcaagt cagcttatct tgaaatgatg cagaaatcat tatcagactt
     2581 aaaagaattt aaaagcggtt tatctccttt agatgaacct tgatgcgaag taacaatt
//
LOCUS       DASWBY010000772         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_712124, whole genome shotgun sequence.
ACCESSION   DASWBY010000772 DASWBY010000000
VERSION     DASWBY010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712124"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VF870_14020"
     CDS             <1..778
                     /locus_tag="VF870_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253358.1"
                     /translation="EKGLSRMLGPANPSSNDEYGMLLEGFEDSPRLLMTYNPNYYLDL
                     CSNYGMTKAKDLLAYKLENHKVMGSEKLKRVQQIARDRYKLKISQLDMKNFAKELEKV
                     KLVYNKAWAPNWGFIPMTDEQIDAMAKDLKPLVEPSLVLFGEIDNQLVGFALVMLDYN
                     HIFKQMNGRLLPFNFIKLFTKKKEIKWARIITLGLIPEQQKKGLDAVFYWEIVNRAHN
                     VGIDLGEASWVLEDNEMMNRGAVVMGGNPYKKYRIWQSDL"
     gene            809..>2093
                     /locus_tag="VF870_14025"
     CDS             809..>2093
                     /locus_tag="VF870_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253359.1"
                     /translation="MRLLILLLLLSKLIFAQYDSAMYDLIKTTYERSFDKTIINKYLL
                     ADSDKEIKAAILSIAQSEDTSFVPGLLKLDLSKYGSEVCFALAQIGKSNQSINFLWKY
                     LHSSPPPNQISNIFFAIGKIGDEKDLKQLVEFYNLFDGPIFPYEGVSEAILQFHIHGI
                     KSDVAKSILETEITHNLSSKKRIEHALFALARFRSSDLTGREFQNLLESEYAKDDEVF
                     RQFALMNVNRQIQIDKKVLAKLFESNSPLTKIQLVKVLHFFEIDSAASSNEIINYYLT
                     LLYDDNQDVSLQAAISIKDIKDLLNDSLKTVLKNKIDYLLFDSNKALSFKGELFLSRY
                     QLFGNYAEHEKLLNELRLSNKYQIQFFGKHPDSEIAFNKLSDFYRNSSELKTKLESLT
                     QILELKSDSKYLTEEFKLLLNSLNSDQAPLISVSAD"
BASE COUNT          737 a          318 c          356 g          682 t
ORIGIN      
        1 ggaaaaaggt cttagcagaa tgcttggtcc ggctaatcca tcaagtaatg atgaatatgg
       61 aatgctgctt gaaggttttg aagattcgcc aagactcttg atgacttaca atcctaatta
      121 ctatttagat ctttgttcaa attacggaat gacaaaggca aaagatcttt tagcttataa
      181 acttgaaaat cataaagtaa tgggttccga gaagttaaaa agagtccagc agattgcacg
      241 tgatcgttac aagcttaaaa tatcgcagct tgatatgaaa aactttgcta aggaattaga
      301 gaaagtcaaa ttggtttata acaaagcctg ggcacccaat tggggattca ttcctatgac
      361 tgacgaacaa atagacgcca tggcaaagga tttaaagcct cttgttgaac cttcactagt
      421 tttgtttgga gaaattgata atcagttagt aggttttgca ttagttatgc ttgattacaa
      481 tcatatattt aaacaaatga atggcagact gcttcccttt aacttcataa agttgttcac
      541 taaaaagaaa gaaattaaat gggccagaat tattactctt ggcttaattc ctgagcaaca
      601 gaaaaaaggt ttagacgcgg tattttattg ggagattgta aaccgtgccc ataatgttgg
      661 aattgattta ggcgaagcaa gctgggtact tgaagataac gaaatgatga atcgtggtgc
      721 agttgtaatg ggtggcaatc cgtataaaaa atacagaatc tggcagtctg atttataatt
      781 gtttttatac tttttatttg aggaataaat gcgtctcctc atcctcttac ttttattaag
      841 caaattaatt tttgctcaat atgattccgc aatgtacgat ttgatcaaaa ctacttatga
      901 gagaagtttt gacaaaacaa ttattaataa gtatctgctc gctgattccg ataaagaaat
      961 taaagctgca atattaagca ttgctcaaag tgaagacact tctttcgttc ctggattatt
     1021 aaaattggat ttatctaaat atgggagtga agtttgcttt gctcttgcac aaataggcaa
     1081 aagcaatcaa tccataaatt ttctttggaa atacttgcat tcctctcccc cgcccaatca
     1141 aatttcaaat atattttttg cgattggtaa aatcggagat gaaaaagatt taaagcaact
     1201 agttgagttt tacaatttat tcgatggacc tattttccct tatgaaggtg tctcagaggc
     1261 tattcttcaa ttccatattc atggaataaa aagtgatgtt gcaaaatcaa ttttagaaac
     1321 tgaaattaca cacaatcttt ctagtaagaa aagaattgaa catgcattat ttgcgttggc
     1381 aagatttaga agttcggatt taaccggcag ggaatttcaa aatttattag aatctgaata
     1441 tgctaaagac gatgaagtat tcagacaatt tgctttgatg aatgtaaata ggcagataca
     1501 aattgataaa aaagttttgg ctaaattatt tgaatcaaac tctccgctta caaaaatcca
     1561 gttagtaaaa gtattgcatt tctttgaaat tgattcagcc gcatcttcaa acgaaattat
     1621 aaattattat ttaactctat tatatgatga taatcaagat gtctcattgc aagcagcaat
     1681 ttcgataaag gatattaaag atctcttaaa tgattcacta aaaactgttc ttaaaaataa
     1741 aatagattat ctgcttttcg attcaaataa agcattaagc tttaaaggtg aacttttttt
     1801 aagccgatat caattgtttg gcaattatgc tgaacatgaa aaattgctaa atgaattacg
     1861 actttcaaat aaatatcaaa tccagttttt tggaaagcat ccggactctg aaattgcttt
     1921 taataaatta agtgatttct accggaattc atctgaacta aaaaccaaat tagaatcctt
     1981 aacccaaata ttggaattaa aatcagattc caaatattta actgaagaat ttaaattatt
     2041 attaaattcg cttaactccg atcaggcccc ccttatatca gtgtcagctg atg
//
LOCUS       DASWBY010000773         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_557628, whole genome shotgun sequence.
ACCESSION   DASWBY010000773 DASWBY010000000
VERSION     DASWBY010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557628"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VF870_14030"
     CDS             <1..375
                     /locus_tag="VF870_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX9253360.1"
                     /translation="VPMSVEALKKGAINFLEKPIDEQTLVKALDEALSRSEELITKNK
                     ELDGIRALMNTLTTREYEIFRYVISGMLNKQIAAELGIAEHTIKNHRLNITDKLKVKS
                     VAELVNIADKLSIKGTVVNHSS"
     gene            500..883
                     /locus_tag="VF870_14035"
     CDS             500..883
                     /locus_tag="VF870_14035"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9253361.1"
                     /translation="MIYIVDNDKNILEGYRMLLKSAGFDCKNFETAEEFLKNFHAEET
                     KLLLLDIHLHGMNGFALLEKLEKEGYHLPVIIITAYDENESRVQARKYGVMAYLRKPI
                     DGEALIDLINYNLDIQKSNERSTIS"
     gene            904..2127
                     /locus_tag="VF870_14040"
     CDS             904..2127
                     /locus_tag="VF870_14040"
                     /inference="COORDINATES: protein motif:HMM:NF039990.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HEX9253362.1"
                     /translation="MKILLSSILVLLLGSLVLAQDADKNWFKIYGFAMTDIGYNANQI
                     HPDWYDVVRPTKLPTIKNQYGTDGNAYFSVRQTRFGVQSGTKTALGELFTQFEWEMFG
                     TGVDAGQTTIRLRHAYGELGQFGVGQYWSPFMDIDVFPNTVEYWGPNGMAFFRNVQFR
                     WMPIKGDTRLTFALERPGASADAGEYSDRVELQGVKARFPVPDLSAEYRSAHDWGYVE
                     LAGIVRYMKWEDQLVSAGEKDLSGDAIGWGLNLSSNLKLGKDNVVRLSFLYGAGVQNY
                     MNDAPVDVAIDGTGDDAKGVAIPLYAAVAFLEHNWSEKFATAIGYSMLNIDNTDGQSY
                     DDFKMGQYAIGNIIYYPVANVMMVAELQYGSRENFKDVPENEPQSVKDSYLKTADILK
                     VQFSFKYNFSHLINY"
     gene            2164..>3058
                     /gene="glsA"
                     /locus_tag="VF870_14045"
     CDS             2164..>3058
                     /gene="glsA"
                     /locus_tag="VF870_14045"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEX9253363.1"
                     /translation="MRKNYFETMLLALMLIVFVLSSPVNVFAQDLSKANIEKVLKEAY
                     DKFKDVKEGANADYIKELATVDPNIYGIALVTTDGQIYTAGDIKSMVSIQSVSKVFTM
                     AKVIEEQGHQAVQDKIGVDATGEVFNSITAVERMRGKEINPLVNPGAIASTSLIAGVD
                     SAAKWKSILQVHSDFAGRQLELNMPVYISEAGDNLRNQAIAHLLFAYGRMYFDPVQAT
                     DIYTKQCAINVSAKDAGIMAATLANGGVNPVTKKKVVSPETVMYTLPVMSTAGLYDDS
                     GIWLYNAGVPAKSGVGGCLIAV"
BASE COUNT         1030 a          460 c          636 g          932 t
ORIGIN      
        1 gttccgatga gtgttgaggc tttaaagaaa ggtgcgataa attttttaga aaaacctatt
       61 gatgaacaaa cattagttaa agccttggac gaagcactca gccgtagtga agaattaatt
      121 acaaagaaca aagaattaga cggaataaga gctttaatga atactctgac cacacgggaa
      181 tatgaaatat ttcgctatgt gatttccgga atgcttaata aacaaatcgc tgccgaatta
      241 ggaatcgccg aacatactat aaaaaaccat cgtttgaata ttactgataa gttaaaggtt
      301 aaatctgtag ctgaattagt taatattgct gataaattaa gcataaaagg gacagtagtt
      361 aatcattctt cttaaagttc aaaacctttt tattcacatc ttcaaaatac ttgaatagga
      421 ctaagtacct atttacccgc tcttttcttt aagctaattt tagttaaaac ataaatatta
      481 atatagaatc gtaaaagaca tgatttatat tgtggataac gataaaaaca ttctagaagg
      541 gtatcgaatg cttcttaaat ctgcaggatt tgattgtaaa aactttgaaa ctgcagaaga
      601 gtttttaaag aacttccacg cagaagaaac taaattactc ctgcttgata ttcatctgca
      661 tggaatgaat ggatttgcat tattagaaaa actggaaaag gaaggctatc atttacctgt
      721 tatcataatt actgcttatg atgaaaacga aagtcgtgta caggccagaa aatatggagt
      781 gatggcttat ttacgaaaac caattgacgg tgaggctttg atagatttaa ttaattataa
      841 tttagatatt caaaaatcaa atgaacgttc aactatttct taaaccaaag taaggagtgc
      901 tctatgaaaa ttcttttatc atcaattctt gttttattac tgggaagtct ggttttagcc
      961 caggatgcag ataaaaactg gtttaaaatt tatggatttg caatgaccga tattggttat
     1021 aatgcaaacc aaattcaccc ggactggtac gacgttgtaa gaccgactaa gctcccgaca
     1081 attaaaaatc aatatggtac agatgggaat gcttatttta gtgttaggca aaccaggttt
     1141 ggtgttcaaa gcggaacaaa gactgcactc ggtgaattat tcacacaatt tgaatgggag
     1201 atgttcggaa ccggtgttga tgcaggacaa acaacaatac gccttagaca cgcatacggt
     1261 gaacttggtc aattcggtgt tggtcaatat tggtcaccct ttatggatat tgatgtattc
     1321 ccgaatacag ttgaatactg gggaccaaac ggtatggcat ttttccgcaa tgttcaattt
     1381 aggtggatgc ctattaaagg tgatacacgt ttaacttttg ctcttgaaag accgggcgca
     1441 agtgctgatg ctggtgaata cagtgaccgt gttgaacttc aaggtgtaaa agcaagattt
     1501 cctgtaccgg atttatctgc tgaatatcgt tctgctcatg attggggcta tgttgaatta
     1561 gctggaatag ttcgttacat gaaatgggaa gatcaattag taagtgcagg tgaaaaagac
     1621 ttgagcggtg atgctattgg ctggggttta aatcttagtt ctaatctaaa attaggtaag
     1681 gataatgtag taagactatc atttctttat ggtgcaggtg ttcaaaacta tatgaacgat
     1741 gcacctgttg atgttgctat agatggaact ggtgatgatg caaaaggtgt agctattcca
     1801 ctttatgctg cggttgcttt tcttgaacac aattggagtg aaaaatttgc aacagcgata
     1861 gggtattcaa tgttaaatat agataataca gatggacaga gttacgatga ttttaaaatg
     1921 ggtcaatatg ctatcggtaa tattatctat tatcccgtgg caaatgtaat gatggtagct
     1981 gagcttcaat acggtagcag agaaaatttt aaagacgtac cggaaaatga accgcaatca
     2041 gttaaagatt catatttgaa gacggcggat atcttaaaag tgcagttctc tttcaaatat
     2101 aatttttcac atttaataaa ttactaatgt tttgaactta taaatcttaa ggagattaaa
     2161 agtatgagaa aaaattattt tgaaacaatg ctgttagcat taatgttgat tgtatttgtt
     2221 ttatcaagcc ctgttaatgt gtttgcacag gatctttcaa aagcaaacat agaaaaagtt
     2281 ctaaaagaag cttatgataa gttcaaagat gtaaaagaag gagcaaacgc cgattacatt
     2341 aaagaacttg caacagttga tccaaatatt tatggaatcg cattagtcac aactgatggc
     2401 cagatctata ccgcaggtga tattaaatca atggtttcaa tacagagtgt ttctaaagtg
     2461 tttacgatgg ctaaagtaat tgaagaacag ggtcatcagg ctgttcagga taaaattggt
     2521 gttgatgcta caggcgaagt tttcaactca attactgccg tcgaaagaat gagaggaaaa
     2581 gaaattaatc ctcttgtaaa tcccggtgct attgcttcaa caagtttaat tgccggtgtt
     2641 gattcagctg caaaatggaa aagcatttta caagtacaca gcgattttgc aggaagacaa
     2701 cttgaattaa atatgccggt ttatatcagc gaagcaggag ataatttacg taaccaggcg
     2761 attgcacatt tactttttgc ctatggcaga atgtattttg atcctgttca ggcaacggac
     2821 atttacacga agcaatgtgc tataaatgtt agtgcaaaag atgccggaat tatggcagct
     2881 actttggcaa atggcggtgt taatcctgtt acaaagaaaa aagttgtatc accggagaca
     2941 gttatgtaca ctcttcctgt aatgtcgact gccggacttt acgacgattc tggtatctgg
     3001 ttatataatg caggcgttcc ggcaaaaagc ggtgtaggcg gatgcttaat agcagtag
//
LOCUS       DASWBY010000774         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_295117, whole genome shotgun sequence.
ACCESSION   DASWBY010000774 DASWBY010000000
VERSION     DASWBY010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295117"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="VF870_14050"
     CDS             <1..1599
                     /locus_tag="VF870_14050"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX9253364.1"
                     /translation="DFRGERFKDYPHDLKGNNDLLSITQPDVIKGIHREYFAAGADIV
                     ETNTFNANVISQADYHLQELAYKINYESAKIAKEVANEFNQPVGKAGNKFPDKPRFVA
                     GALGPTNKTLSLSPNVNDPGYRAVTFDEVAAAYYDAACGLVDGGADIILIETIFDTLN
                     AKAAIFGVEKLITERSLDIPVMISGTIVDQSGRTLSGQTTEAFLISVSHAKNLVSVGL
                     NCALGAKQMRPFVEDLSNVSDKFLSVYPNAGLPNEMGGYDETPQTMATVLEDFLASGF
                     VNIVGGCCGTSPDHIREISEIIKKHKPRIPKVQEPYLRLSGLEPVVYRPDSNFMNIGE
                     RTNVTGSKKFARLIKEDNYDEALSVARDQVEGGAQVLDINMDEGMLDSEAAMTKFINL
                     LEAEPDIAKLPIMIDSSKWSVIEAGLKCLQGKGIVNSISLKEGEDVFKEHARKVLSYG
                     AAVIVMAFDEKGQADTFERRIEICKRAYKILTEEIGFQPQDIIFDPNILAIATGIEEH
                     NNYAVDYLEATRWIKQNLPYAKVS"
     gene            1654..>3091
                     /locus_tag="VF870_14055"
     CDS             1654..>3091
                     /locus_tag="VF870_14055"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX9253365.1"
                     /translation="MHSAFLFHAVKAGMDMGIVNAGQLEVYEEIQKDLLEKVEDVILN
                     RKPDATERLIEFAETIKKKDKSSIGEEKKENWRNAPVEERLKHALVKGIVDFIDVDIE
                     EARQKYPQPIEVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKKAVAILIPFIEA
                     EKEKNKSTREQGKILMATVKGDVHDIGKNIVGVVLGCNNYNVIDLGVMVHTEKILQTA
                     VDEKVDVIGLSGLITPSLDEMVHVAKEMERRGMKLPLLIGGATTSRIHTAVKIVPNYS
                     GAVIHVLDASRSVPVVSNLLNDNEEERKKYVQSFKDEYAQAREDYSKRKSDKNYISLD
                     RARENRLKIDWQKTKIKKPNKVGITTLKEFSLEKLRSYIDWTPFFMTWELKGKYPAIF
                     DDKNVGPEAKKIFDDANKLLDKIISDKLLTANGVIGLFPANSVGCDDIEIYADESRSG
                     VKRVLHTIRQQIEKSKDEPNIALADFIAP"
BASE COUNT         1072 a          482 c          680 g          857 t
ORIGIN      
        1 gattttcgtg gtgagcgatt taaagattat ccacacgatc taaaaggcaa taatgatctt
       61 ctttcaataa cacaaccgga tgtaataaaa ggaatccaca gagaatattt tgccgcaggt
      121 gcagatattg ttgaaacaaa tacgtttaat gcaaatgtta tttcgcaagc tgattatcac
      181 ttacaagagc ttgcgtataa gataaattat gaatcggcaa agattgcaaa agaggttgca
      241 aatgaattta accagcctgt tggcaaggca ggtaacaagt ttccggataa acctcgattt
      301 gttgcaggtg cattgggccc aacaaataaa acactttcac tatctccaaa tgttaatgat
      361 cccggttacc gggctgtaac ctttgatgaa gttgctgcag catattatga tgcggcttgt
      421 ggtttggttg atggcggcgc agatataatt cttattgaaa caatatttga tacgctaaac
      481 gcaaaagctg caatttttgg cgttgaaaaa ttaattaccg aaagatcgct tgatattccg
      541 gtaatgattt caggtactat cgttgatcag agcggaagaa cactatccgg acaaacaaca
      601 gaagcatttt taatatcagt ttctcatgca aaaaatcttg ttagcgttgg attaaattgt
      661 gctcttggtg caaaacaaat gagaccattt gttgaggacc tttcaaatgt gtcggataaa
      721 tttttatctg tttatccaaa cgccgggctt ccaaacgaaa tgggcggata tgatgaaact
      781 ccgcagacaa tggcaactgt gcttgaagac tttcttgcca gtggatttgt aaatattgtt
      841 ggtggttgct gcggtacaag tccggatcat ataagagaaa tttcagaaat tataaaaaaa
      901 cacaaaccaa gaattcctaa agttcaggaa ccgtatttaa gattaagcgg tcttgagccg
      961 gttgtatatc gcccggattc caactttatg aatattggcg agcgtacaaa tgttacagga
     1021 tcaaaaaagt ttgcacgcct tattaaagaa gataattatg atgaagcact ttctgttgcc
     1081 cgtgatcagg ttgaaggcgg tgcgcaggtt ttagatatta atatggatga aggcatgctg
     1141 gattccgaag ctgccatgac aaagtttatt aatcttcttg aagcagaacc ggacatcgca
     1201 aaacttccta taatgattga ttcttctaag tggagcgtaa ttgaggcagg attaaaatgt
     1261 ttgcaaggga agggaattgt aaactcaatt tctcttaaag aaggcgaaga tgtatttaaa
     1321 gagcacgcaa gaaaagtact aagttacgga gccgcagtta ttgtaatggc gtttgatgaa
     1381 aaaggacagg cagatacatt tgagagaaga attgaaatct gcaagcgggc atataaaatc
     1441 ttaactgaag aaattggctt tcagccgcag gatataattt tcgatccaaa cattcttgca
     1501 attgcaactg gaatagaaga acacaataat tatgctgttg attatcttga agcgacaaga
     1561 tggataaaac aaaacctgcc gtatgcaaaa gtaagctgag gagtcagcaa cctttctttt
     1621 tcattccgag gtaacgatac agtccgtgaa gcaatgcatt ctgcttttct ttttcatgcg
     1681 gttaaagccg gcatggatat gggaattgtt aatgctggtc agcttgaagt ttatgaagaa
     1741 attcaaaaag atcttttaga aaaagttgaa gatgtaattc ttaatagaaa gccagatgct
     1801 accgagcggt taattgaatt tgcagagaca ataaaaaaga aagataaatc ttccattggt
     1861 gaagagaaaa aagaaaattg gagaaatgca ccggttgaag aaaggctaaa acatgctctg
     1921 gttaagggta tagttgactt tattgatgta gatattgaag aagctagaca aaaatatccg
     1981 cagccgatag aagttataga aggccctcta atggcaggaa tgaacgtggt gggagatcta
     2041 tttggcgcag gaaaaatgtt tttgcctcag gttgtaaaaa gcgctcgcgt tatgaagaag
     2101 gcggtggcaa tattaattcc gttcattgaa gctgagaaag aaaaaaataa aagcacacgt
     2161 gaacaaggca agattttaat ggcaacagtt aaaggtgatg tgcatgacat tggaaaaaac
     2221 atagttggcg ttgtgcttgg gtgtaacaac tataatgtta ttgatcttgg agtaatggtt
     2281 catacagaaa aaattcttca gacagctgta gatgaaaaag ttgatgttat tggtttaagc
     2341 ggacttatta ctccgtcgct tgatgaaatg gtacacgttg caaaagaaat ggaaagacgc
     2401 ggaatgaaat tgcctttact tattggcggc gcgactactt cacgcattca caccgcagtt
     2461 aaaattgtac ccaactatag tggtgcagtt atacatgttc ttgatgcgtc tcgcagtgtt
     2521 ccggttgtat caaacctttt aaatgacaat gaggaagaaa gaaaaaaata tgtgcagtcg
     2581 tttaaagacg agtatgctca ggcaagagag gattattcca aacggaaatc tgataaaaat
     2641 tatatctcgt tggatagagc aagagagaat agactaaaaa ttgattggca gaaaacaaaa
     2701 attaaaaaac caaataaagt aggaataact actcttaaag aatttagtct ggaaaaactc
     2761 cgcagttata ttgattggac tccattcttt atgacgtggg aattaaaagg caagtatccg
     2821 gcaatatttg atgacaagaa cgttggacca gaagccaaga aaatttttga tgatgcaaat
     2881 aaacttcttg ataaaataat ttcagataaa ttacttaccg caaatggagt tattggatta
     2941 tttcccgcaa attctgttgg ctgtgatgac atagaaatat atgcagacga atcgcgaagc
     3001 ggggtaaaaa gagttttgca tacaataaga cagcaaattg aaaaatccaa agatgaacca
     3061 aatattgcac ttgcagattt tatcgcacca a
//
LOCUS       DASWBY010000775         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_520265, whole genome shotgun sequence.
ACCESSION   DASWBY010000775 DASWBY010000000
VERSION     DASWBY010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520265"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VF870_14060"
     CDS             complement(<1..1232)
                     /locus_tag="VF870_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253366.1"
                     /translation="MKKHFLLNIILIAFFCNSNSIYAWVYPEHRDIALIAIQNLNPEY
                     RKVLDALWAEARKGYEFRLTEAVIDATQSTNPTQLDYVSWPAISGDHSCSAKNMLHNV
                     LETEWILKVADITAQLKIDIANAKDRIARINALRESDLKLQSADPEYATRAGSNNVHF
                     LLPLADPSIKLDTYIFRCLAEEAELNALGIYSWYHSRAIAKISRINSNNLTPEERSVL
                     ILSALADEAFALHFLQDVFAAGHVAGTRGDASQRKGTHDYYNEYGLKVSTWEGESLVL
                     TGDAWMRHEDSEIASIAVRMSLEAFLKAAINGFNDQPILNEINLTTPEDFNVCTNNFM
                     PAIKTGTNFILSLQPIFMKTPIPGLSQGLGEFPRFRAELGAFVGFSPSVRGWLFSGGF
                     GNGQETIGVSGGLEVAAR"
     gene            complement(1311..2297)
                     /locus_tag="VF870_14065"
     CDS             complement(1311..2297)
                     /locus_tag="VF870_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9253367.1"
                     /translation="MKFIPSYIKLLHSGELYKRAEKAFENLSSCTSCPRDCNVDRING
                     EYGICTSGHLPIVSSYTPHFGEEPVLSGTKGAGNIFFGNCNLKCIYCQNFEISQNPKS
                     EKHHEVTHDRLAGIMIELQNKDCHNIGLVSPTHFSATILKSIYIAAEKGLKLPIIYNT
                     NGYDSVEMLKLYDGVADIYLPDFKYGNSEYAKTYSLVDNYFEKTKEAIKEMFRQVGDE
                     LIYDGDVVVRGMIIRHLILPNDLSDTEKIFKFIAEELSPKIHISLMSQYYPINKAYKD
                     ILIDRAVRVSEYEKALNLLDKYGLHNGWIQELESSNTYLPNFNENRANPFKN"
     gene            complement(2378..>2933)
                     /locus_tag="VF870_14070"
     CDS             complement(2378..>2933)
                     /locus_tag="VF870_14070"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9253368.1"
                     /translation="KEPQDKRPYSKAVWHYARAMALIAKNNIIEAEKEIAELKTLRNN
                     KSVEELSIWGINSAGNLIKIAYEIAQGELNAKKKNYSDAIANLNKAVEIEDQLRYDEP
                     PTWFYPCRQNLGAVLIEAGKYAEAEKVYMENLKEVPENGWALFGLHQAMLKQNKLDEA
                     AEIEKRFNDAWKYADVKLTASRIM"
BASE COUNT          859 a          564 c          519 g          991 t
ORIGIN      
        1 aaacgcgccg ctacctccag tccacctgaa actccgatag tttcttgtcc gtttccaaaa
       61 cctccggaaa ataaccagcc gcgcactgag ggtgaaaacc caacaaaagc acccagctcg
      121 gctctaaatc tgggaaactc ccccaagcct tgtgataacc cgggaattgg cgttttcata
      181 aatattggtt gcaatgacaa aataaaatta gtgcctgttt ttattgcagg cataaaatta
      241 ttggtgcaca cattaaagtc ttccggcgta gttagattga tttcattaag aatgggctga
      301 tcattaaacc cattgattgc ggctttaaga aaagcttcca gactcattcg tactgcaatg
      361 gatgctattt cgctatcctc atgtctcatc caggcatcgc ctgttaatac aagactctct
      421 ccttcccatg tacttacttt aagtccgtat tcgttgtaat agtcatgagt accttttctc
      481 tgtgaagcat caccacgggt tcctgcaaca tggccggcag caaaaacatc ctgaagaaag
      541 tgcaaagcaa aagcttcatc ggcaagtgct gaaagtatca ggacagagcg ttcttcagga
      601 gttagattat ttgagtttat tcttgaaatt tttgctatcg cccgggaatg ataccaggaa
      661 taaataccca aggcattaag ttctgcttct tcggcaaggc atcgaaaaat gtaagtatca
      721 agtttaattg aaggatcggc taatggtaaa agaaaatgca cattatttga acccgctctt
      781 gttgcatatt ccgggtcagc agattgtaat ttaaggtcgg attctcttag agcattaatt
      841 cttgcaattc tatcttttgc atttgcaatg tcaatcttta attgtgcggt aatatcagca
      901 acctttaata tccactcggt ttcgagcaca ttgtgcaaca tgttttttgc tgaacaagaa
      961 tgatcgccag atattgctgg ccaggagaca taatcaagtt gagtggggtt tgtgctttgt
     1021 gttgcatcaa ttactgcttc cgttaatctg aattcatatc cttttcttgc ttcagcccaa
     1081 agcgcatcaa gtaccttgcg atattcggga ttaaggtttt gaattgcgat taatgcaatg
     1141 tctctgtgct caggatatac ccaggcgtat attgaatttg aattacaaaa aaacgcaatt
     1201 aaaataatat tgagtaaaaa atgttttttc aaaattactt tttaaatgat tgatgggaat
     1261 ttgtggcaaa agattgcaaa tttttattta tagaacaatc taagtttaaa ttaattttta
     1321 aaaggattgg ctctgttttc gttaaaattc ggcagataag tattagaact ttccagttcc
     1381 tgaatccagc cattatgtaa accgtatttg tctaacaaat taagagcttt ttcatattca
     1441 gaaacccgta cagcacggtc gattaaaata tctttatacg ccttgttaat tggatagtac
     1501 tgcgacatta atgagatgtg aattttgggg ctaagttctt ctgcaataaa tttaaaaatt
     1561 ttctctgtgt cggataaatc attcggtaaa ataagatgcc ttatgatcat cccgcgtaca
     1621 acaacatcac catcgtaaat aagttcatca ccaacctggc ggaacatttc tttaattgct
     1681 tctttggttt tttcaaaata attatcaacc aaagaatatg ttttggcata ctcactgttt
     1741 ccatatttaa agtccggcag ataaatatca gcaacaccat cataaagttt taacatttca
     1801 acagaatcat atccgtttgt gttgtaaatg attggaagct ttaacccttt ttctgcggca
     1861 atgtaaattg attttaaaat tgttgctgaa aaatgtgtcg gtgaaacaag tccgatatta
     1921 tgacaatctt tgttttgaag ttctatcatt atccctgcta aacgatcatg agttacttcg
     1981 tgatgttttt ctgattttgg attttggctt atctcaaagt tctggcagta gatacatttt
     2041 aagttacagt taccaaaaaa aatattgccg gctccttttg tgccggataa aaccggctct
     2101 tctccaaaat gcggagtgta agatgaaaca attggtaaat gtccgcttgt gcaaattcca
     2161 tattcaccat ttatacggtc aacattacaa tcacgcggac agcttgtgca ggaagaaagg
     2221 ttttcaaaag ctttttcggc acgtttataa agttcgccgc tgtgtaaaag ttttatatac
     2281 gatggaatga atttcataac ataaatttac catccaaact ttataaataa gaatccacaa
     2341 cggcgtatac aggcaaatta ttttaaatta atatcattta cataattctt gaagctgtta
     2401 atttaacatc ggcatatttc catgcgtcgt taaatctctt ttcaatttca gcagcttcat
     2461 caagtttgtt ttgtttaagc attgcctggt gtaaaccaaa taatgcccag ccattttcag
     2521 gcacttcttt tagattttcc atataaactt tttctgcctc cgcatatttt ccggcttcaa
     2581 tcaaaacagc tccaagattt tgccggcatg gataaaacca tgttggtggt tcatcgtatc
     2641 gcagctgatc ttcgatctca acagctttgt ttagattagc aattgcatcg gagtagttct
     2701 tcttctttgc atttaattct ccttgagcta tttcatatgc aattttaatt aagttacctg
     2761 cagagttaat tccccagata gaaagttctt ctaccgattt attatttcgt aaagttttca
     2821 gttcggcgat ttctttttct gcttctataa tattgttctt tgcaattaat gccatcgcgc
     2881 gtgcataatg ccagactgct tttgagtatg ggcgtttatc ttgcggttcc tta
//
LOCUS       DASWBY010000776         3998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_329692, whole genome shotgun sequence.
ACCESSION   DASWBY010000776 DASWBY010000000
VERSION     DASWBY010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329692"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VF870_14075"
     CDS             complement(<1..445)
                     /locus_tag="VF870_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX9253369.1"
                     /translation="MLKRFEKIKNINGELNLNGDKSISHRAVIFSAMASGKSLIKNIS
                     PGEDVKSTLKIMQQLGAEVLNEDEQTIIEGCGFKGFKEPTNNLDCGNSGTSARLISGL
                     LAAQDFSSTIIGDDSLSKRPMKRVIEPLKQMGIEFNSNQNLTLPLT"
     gene            complement(445..1035)
                     /locus_tag="VF870_14080"
     CDS             complement(445..1035)
                     /locus_tag="VF870_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX9253370.1"
                     /translation="MRSTKTEKRLEKITRVIKSRQHSLTVVMENIHDPHNVSAIFRTC
                     DAVGIPKVNLVYNFETFPRIGKKSSASAFKWVDKEKFKTIEDCYSELRKEGFKIFASS
                     LTENSKNLYDLDLTQKVAIVVGNEHRGVSEASTELADEVFLIPQFGMVQSLNVSVATA
                     VILYEAMRQRIKKGMYKKSELDEKTLDKLIDNWCEK"
     gene            complement(1041..1262)
                     /locus_tag="VF870_14085"
     CDS             complement(1041..1262)
                     /locus_tag="VF870_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253371.1"
                     /translation="MVIDLIVTKTNDGYTAEVPSIKGCESWAHDEDTVIEKIIELTSF
                     YVRQPAKEIKLDRARREENKIIYKLIFHK"
     gene            complement(1389..2162)
                     /gene="deoC"
                     /locus_tag="VF870_14090"
     CDS             complement(1389..2162)
                     /gene="deoC"
                     /locus_tag="VF870_14090"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX9253372.1"
                     /translation="MSWKDLIAEENLNKILNEFSQFEQSKKISEIKISSNDLACMIDH
                     TLLKPDATSSEIKQLCEEALQNNFASVCVNPSYIDACFNLIKSSNVKVCTVIGFPLGA
                     TTTQSKFLEAEEAIKNGAEELDMVINIGKLKDKNYEYVFNDIKSIADLSRKHLCISKV
                     ILETCLLTDNEKAAACLLAKEAGMNFVKTSTGFSNSGANVHDVTLMKFVVGDKLQVKA
                     SGGIRSYEDAIAMINAGATRLGASAGVKIIAGQKSESSY"
     gene            complement(2163..3041)
                     /gene="folD"
                     /locus_tag="VF870_14095"
     CDS             complement(2163..3041)
                     /gene="folD"
                     /locus_tag="VF870_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX9253373.1"
                     /translation="MILIDGKKVAADIRNELKEKISQLKAEGKNAPGLVAIIVGDDPA
                     SQIYVASKGKACEEIGMRTKTEKLNADISEAELLSLIQYYNQNNDYHGILVQLPLPIH
                     INEDKVIETISYKKDVDGFHPISVGNLVIGKDTFASCTPAGIQELLIRYKIETKGKHV
                     VVVGRSNIVGKPIANIMLQKADGANSIVTIVHSAAKDISYYTKQADILIAAIGKAEMI
                     KEDMVKDGVAVIDVGINRIEDSAKAKRYRIVGDVDFENVSKKASFITPVPGGVGPMTI
                     AMLLSNTFKAYKLYGE"
     gene            complement(3049..>3998)
                     /locus_tag="VF870_14100"
     CDS             complement(3049..>3998)
                     /locus_tag="VF870_14100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX9253374.1"
                     /translation="EKLRKLLLPYSRFNEGTIWIDEKAGHKIACLDVSKSDEVKKIFG
                     KSKVKLSVQDPPYNFIAFTEKQNDDFIKWCKRWITLNKEFSDKNSSFYFWLGADQSNH
                     FEPFGGFIEMMKSTGLASRSFITMRNQRGYGTQHNWMAIRQELLYYTNGKPKFNIKSE
                     YTDIPKVLKGYYKEVGGDITDNMQRSKSQFIRAGNVWIDVQQVFYRIEENVNGCFAQK
                     PLKAIKRIVEASSNKKDLVTDFFSHSGTTLIATELLGRKCITFDYDPVYCEITLRRLE
                     NYRETNKTGWQNSNPFYSEIKEDKKILNHLKKKYNITYK"
BASE COUNT         1193 a          771 c          561 g         1473 t
ORIGIN      
        1 tagttaaagg cagagttaaa ttttgattcg agttaaactc aatacccatt tgtttgagag
       61 gttcaataac tcttttcatt gggcgcttag aaagtgaatc atcaccaatt atagttgaac
      121 taaaatcctg agctgccagc aatcccgaaa ttaatcttgc tgaagttccg gaattaccgc
      181 aatctaaatt attagttggt tctttaaatc ctttaaaacc gcatccttca attattgtct
      241 gttcatcttc attcaaaact tctgcaccta actgctgcat aatctttaaa gtagatttaa
      301 catcttcacc aggtgaaata ttttttatta aggactttcc tgatgccatt gcagaaaata
      361 ttacagcacg atgagaaatt gatttgtcac cgtttaaatt taattccccg ttaatgtttt
      421 taattttctc aaatcttttt aacattattt ttcacaccaa ttatcaatta acttgtcaag
      481 agttttttca tcaagttcgc ttttcttata catgcctttt ttgattcgct gtcgcattgc
      541 ttcataaaga attacggccg tagcaacgga aacatttaga ctctgtacca ttccaaattg
      601 cggaattaaa aatacttcat cggctaattc tgtggaggct tctgaaactc cccggtgttc
      661 attgccaaca actattgcaa ctttttgggt caaatccaga tcatatagat ttttagaatt
      721 ttctgttagc gaagatgcaa atattttaaa gccctctttc cttaattcgc tataacaatc
      781 ctcaatcgtt ttaaactttt ctttatcaac ccacttaaat gccgatgctg aagatttttt
      841 accaattcgc ggaaatgttt caaagttata cacaaggtta acttttggaa ttccaactgc
      901 atcacacgtc ctgaaaattg ctgaaacatt atgcggatcg tgaatatttt ccatcacgac
      961 tgttaaagaa tgctggcggg attttataac tcttgtaatt ttctcgagtc gtttttcagt
     1021 tttagtactt ctcacaggaa ttatttatga aaaattagtt tataaattat tttgttttct
     1081 tctctgcggg ctctatccaa tttaatttct ttagcaggct gcctaacata gaacgaagtc
     1141 agttctataa ttttctcaat gactgtatct tcatcgtgtg cccagctttc gcatcctttt
     1201 atagagggaa cttcagctgt gtacccgtca tttgttttcg ttactattaa atctataacc
     1261 atgaaatttc tttataatat aagggtcatt gcgagcgaac gaagcaacct attattctac
     1321 agtttgcttc aaaccaacaa acggtctgtc gatacgcaga ctccttcgaa atgactttat
     1381 taaataagct aataactgct ttctgatttc tgtcctgcaa taatttttac accagcactt
     1441 gcaccgagtc tggttgcacc tgcattaatc attgcaatcg catcttcata agaccggatt
     1501 cctccagatg cttttacttg taatttatct ccaacaacaa atttcatcaa tgttacatcg
     1561 tgtacattcg ctccgctgtt agaaaaccca gttgatgttt ttacaaaatt cattcctgct
     1621 tctttggcta ataagcaagc tgctgccttt tcattatctg tgagcaggca agtttctaaa
     1681 attactttag aaatacataa atgttttctg gataaatctg caattgattt tatatcatta
     1741 aacacatatt cgtaattttt atctttaagt tttccgatgt ttataaccat atccagttct
     1801 tcagcaccat ttttaatagc ttcttcagct tcaagaaatt ttgattgagt agttgttgca
     1861 cctaaaggaa accctataac tgtgcagact ttaacatttg aagacttgat taaattaaaa
     1921 caagcatcta tatatgatgg attaacacaa accgaagcaa aattattttg taaagcttct
     1981 tcacaaagtt gtttgatttc agagcttgtt gcatctggtt taagtaaagt atgatcaatc
     2041 atacaggcta aatcatttga agatattttt atctcggata ttttcttcga ttgttcaaac
     2101 tgagaaaatt catttaatat tttatttaaa ttttcctctg caattaaatc tttccaactc
     2161 atttattcac catataattt ataagcttta aaagtattgc tcaataacat tgcgattgtc
     2221 attgggccaa ctccgccggg aacgggagtt atgaatgatg ctttttttga gacattttca
     2281 aaatcaacat caccaactat tctatatctt ttagcttttg ccgaatcttc aattctattg
     2341 attccaacat caataactgc aactccatct ttaaccatat cttcttttat catttccgct
     2401 tttccgattg cagcaattaa aatgtctgcc tgcttcgtgt aataagaaat atctttagca
     2461 gctgagtgaa caattgttac aattgaattt gctccgtctg ctttttgaag cataatgttt
     2521 gcgattggtt tacctactat gttgcttctt ccaaccacaa caacgtgttt acctttagtt
     2581 tcaattttat aacgaatcaa taattcctga attcctgccg gagtacaaga agcaaaagta
     2641 tcttttccaa ttacaagatt accaacggaa atcggatgaa atccatcaac atctttttta
     2701 taggaaattg tttcaattac tttatcttcg tttatgtgaa ttggtaatgg aagttgaaca
     2761 agtatcccgt gataatcatt gttttgatta taatactgaa taagagataa aagttctgct
     2821 tcagaaatat cagcatttaa cttttctgtt tttgttcgca ttccaatttc ttcgcaggcc
     2881 ttacctttac ttgcaacata aatttgtgaa gctggatcat ctccaacaat tattgcaaca
     2941 agacccggag cattctttcc ttcagcttta agctgagata ttttttcctt aagttcattg
     3001 cgaatatctg cagcaacttt ttttccgtca attaaaatca tagccaactc atttataagt
     3061 aatgttatat tttttcttta agtgatttaa tattttttta tcttctttaa tctcagaata
     3121 aaatggatta gaattttgcc agccggtttt attagtctcc ctataatttt ctaatcttct
     3181 caatgtaatt tcacagtaaa caggatcata atcaaaagta atacactttc ttccaagtaa
     3241 ttcagtagct attaatgttg tacctgaatg tgaaaagaaa tctgttacca aatctttttt
     3301 atttgatgat gcttcaacaa ttcttttaat agcttttaat ggtttttgcg caaaacaacc
     3361 gtttacattt tcctcaattc gataaaaaac ctgctgtaca tcgatccaaa catttcccgc
     3421 acgaataaac tgcgatttac tacgctgcat attgtctgta atatcgccgc caacttcttt
     3481 ataatatcct tttaaaacct taggaatatc agtgtattca gattttatat taaacttcgg
     3541 ttttccattt gtgtaataga gtagctcctg ccgaattgcc atccagttat gttgtgtacc
     3601 gtatccacgc tgatttctca tagtaataaa gctgcgggaa gctaatccag ttgatttcat
     3661 catttcaata aatccgccaa acggctcaaa atgatttgac tgatctgccc caagccaaaa
     3721 ataaaaagat gaattcttat cagaaaattc cttgtttaga gtaatccatc tcttacacca
     3781 ctttataaaa tcgtcatttt gtttttcagt aaatgcgata aaattgtatg gcggatcctg
     3841 aacagaaagc ttaactttac tttttccaaa aatctttttt acttcatcac ttttgctgac
     3901 atccaaacag gcaattttgt gtcctgcttt ttcatcaatc cagattgttc cctcattaaa
     3961 tctggaataa ggaagaagca gttttcttag cttttcat
//
LOCUS       DASWBY010000777         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_596091, whole genome shotgun sequence.
ACCESSION   DASWBY010000777 DASWBY010000000
VERSION     DASWBY010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596091"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VF870_14105"
     CDS             complement(<1..778)
                     /locus_tag="VF870_14105"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9253375.1"
                     /translation="MRKLLLFSILALHIFSIPIFAQVGKITGIVKDASTGEALIGANV
                     LIEGTTMGAATNVDGYFVILNVPPNTYNLKASMVGYAPGVYQDVRVNIDQTTEANFNL
                     SSNTFQTDEVVVIATTPIVQKDVSSSRVNLNVAEIENLPVSSIEGVIGLQAGIRTGSN
                     GELEIRGGSQNQTAFLVNGVTLRDERDNSPFTGISYAAIDQVQIQTGGFNAEYGNVRS
                     GLVNVVTKEGSRDKYSFSLISRYRAANQKHFGSSPNDPNSY"
     gene            complement(978..>2277)
                     /locus_tag="VF870_14110"
     CDS             complement(978..>2277)
                     /locus_tag="VF870_14110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX9253376.1"
                     /translation="SEAIKLIAKYSSTDYTLIVLTEKAVMPGQFCLDRFYQVAINTNA
                     AMVYSDYLEQEKGKPIPHPVIDYQQGSLRDDFDFGEILFIKTEFLKKIAANNTGELKF
                     AGLYNLRLQLSQLGPVYRIPEYLYTIESAEEVGTEDKHFSYVDPKNREVQIEMEKVVT
                     QHLMDIGAYVHPPFKEIDLSRSKGFEFEASVIIPVRNRVKTIGDAIESVLKQNTNFKF
                     NLIVIDNHSTDGTSEVISSFAKKDDRLIHYIPERNDLGIGGCWNAGVHHNKCGKFAVQ
                     LDSDDIYKDENTLQTIVDTFHKEKCAMVIGSYQITDFNLKELPPGLIDHKEWTDDNGP
                     NNALRINGLGAPRAFYTPVLREIKIPNVSYGEDYSVGLAISRQYKIGRIYHSLYMCRR
                     WEGNSDSQLDILRINANNVYKDRIRTFELLARKKNKSKIN"
BASE COUNT          677 a          447 c          360 g          793 t
ORIGIN      
        1 aatatgaatt cggatcatta ggagaactac caaaatgctt ctgatttgct gctctgtaac
       61 ggcttattaa actaaatgag tatttatctc ttgaaccttc ttttgtaaca acattaacta
      121 aaccagatct aacatttccg tattcagcat taaatccgcc ggtttgtatt tgaacctgat
      181 ctattgcggc gtagcttatt ccggtaaaag gtgagttatc tctttcatct cttaaagtaa
      241 ctccgtttac caaaaaagct gtttggtttt gtgaaccgcc tcttatttca agttcaccgt
      301 ttgaacctgt tctgatacca gcttgcaatc caatcacacc ttcaatactt gaaacaggca
      361 agttttcaat ttctgccaca ttcaggttta cacgacttga tgaaacgtct ttttgtacga
      421 ttggtgtggt tgcaattact accacttcat cggtttggaa cgtattagaa cttaaattaa
      481 aattagcttc tgttgtttgg tcgatgttta cacgcacatc ctgataaacg cccggtgcat
      541 acccaaccat tgaagctttt agattatatg tatttggagg aacattaaga attacaaaat
      601 atccatccac atttgttgcg gcacccattg tggtaccttc gatcaatacg tttgcaccaa
      661 taagggcctc gcctgttgaa gcgtctttta ctatgccagt aattttgccg acttgtgcaa
      721 agatgggtat tgaaaaaata tgtagtgcga gaatgctaaa gagtagcagt tttctcatat
      781 attactccta ttgttagtct cttcgatcct tgtgggagta gttctgatga aggataaatt
      841 taatatcctt ttttctcaga caattattaa tcagaacaaa ataaaattga ttaaattttc
      901 tgccaaaaaa ctaaataaat tagtttctaa agtcaattgt ttttctaaat aagattattt
      961 gcttcgtttt attaaactca gtttattttt gatttatttt tttttcgagc taataattca
     1021 aaagttctta tgcgatcttt ataaacattg tttgcattaa ttcttaaaat gtctaattgc
     1081 gaatctgagt taccttccca tctgcggcac atatataatg agtgatatat ccttccaatt
     1141 ttatattgtc tgcttattgc taatccgaca gaataatctt caccataact tacatttgga
     1201 atttttattt cacgcagcac cggagtataa aatgcacgcg gggcaccgag tccatttatt
     1261 cttaaagcat tgtttggtcc gttgtcatca gtccattctt tatgatcaat cagtccggga
     1321 ggaagttctt taagattaaa atcagttatc tgataagatc caattaccat tgcacatttt
     1381 tctttatgga aagtatccac aattgtctgc aaagtatttt catccttata aatatcatca
     1441 ctgtctaatt gcactgcgaa ctttccgcac ttattgtgat gaacacctgc attccagcag
     1501 ccgccaatac caagatcatt tctctcaggg atataatgta taagtctgtc atccttttta
     1561 gcgaatgatg atatgacttc actggttcca tccgttgaat gattatcaat tacaattaag
     1621 ttgaatttaa aattagtatt ttgcttaagc accgattcaa ttgcatcacc aatagtctta
     1681 acacgatttc taaccggaat gataaccgat gcctcaaatt caaaaccttt cgaccgactt
     1741 aaatcaattt ccttaaatgg aggatggaca taagctccta tatccatcaa atgctgtgta
     1801 acaacttttt ccatttcaat ttgcacttcc ctgttcttcg gatcaacata actgaaatgt
     1861 ttatcctctg taccaacttc ttcagcactt tcaattgtgt aaagatattc tggtatgcga
     1921 taaacagggc caagctgcga aagttgtaat cttaggttgt acaatccggc aaactttaat
     1981 tcaccagtat tattcgccgc aatttttttc agaaattctg ttttgataaa aagtatttca
     2041 ccaaaatcaa aatcatcgcg caaacttcct tgctgataat ctataacagg atgaggaata
     2101 ggtttgccct tttcctgttc taaataatca gaataaacca tggcagcatt agtatttatt
     2161 gcaacctgat aaaatctatc cagacagaac tggccgggca tcactgcctt ttcagtcaga
     2221 acaattaagg tgtaatcagt ggacgaatac tttgcaatca gttttattgc ttcgctg
//
LOCUS       DASWBY010000778         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_260071, whole genome shotgun sequence.
ACCESSION   DASWBY010000778 DASWBY010000000
VERSION     DASWBY010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260071"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..803)
                     /locus_tag="VF870_14115"
     CDS             complement(321..803)
                     /locus_tag="VF870_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253377.1"
                     /translation="MKKLLLSAIFLLGTFAIHAQTADDMIELLKSDVKTNRKAIITES
                     MAFTEAESKAFWPVYNEFEIEMEKLANKRIQNIKDYAANYDKMTDEKADQLMKAAFDF
                     KSSRVKLNESYYKKFSKVLTPTRAAKYMQIEDQIQLLVDLSIASELPLVKKPVSDAVK
                     "
     gene            complement(905..1534)
                     /locus_tag="VF870_14120"
     CDS             complement(905..1534)
                     /locus_tag="VF870_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253378.1"
                     /translation="MKIKYITALLVLISFTSFAQELLTNVTWNIGIPVSKMNQYTTDV
                     TYQGFTISGRRFLDKYNSVGFMTGWNVFNEKTNTPIDLAGGENASGTLSGTQVRSVNS
                     FPLLVGLHHHYGKRDDMRAFIGLNAGMYYILQRLDIGVYRIDNDNWHFGLAPEAGLII
                     PFDGDNTSFYFGARYNYAFDSGTALNGSENNFYSFYEINLGFSFSSKWF"
     gene            complement(1573..>2057)
                     /locus_tag="VF870_14125"
     CDS             complement(1573..>2057)
                     /locus_tag="VF870_14125"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX9253379.1"
                     /translation="KTDGATFFMPDSIKRILDNNGSVSGSTGQYDAQILAEIERQMIA
                     YGYTRVNTPQEADVFVTVGATSSENIVYYPGYWYGWYGWYYPWYGYGGYAYSYTTGSL
                     FVTMIDKSKIDETNKVTGAVWAGAVNGILDDTNANVLTRVINSVDKMFEQSPYLKIIE
                     "
BASE COUNT          658 a          368 c          298 g          733 t
ORIGIN      
        1 caatccaatc tcatttccga tatctaataa ataataaaat attcataaca tattatttta
       61 taagaattta ggcctttcga tttaattagt attattgaat gtttcagacc tttaaatttg
      121 atatatcatc tggaatgatc aataatttat taagctataa gattgtgaat atccgaatag
      181 aataattaat tcttaacatg cgtggtaatg aggatcaatg attaattaat tgtgaatgtg
      241 ggaagctatt atgaaaaaaa taattggtgt gaataaaaaa aggaggaata ttcttcctcc
      301 cttttctcaa ataagaccat ttattttaca gcgtcactaa ccggcttttt aacaagcggt
      361 aactccgatg caattgaaag atcaacgagt aactgtattt gatcttctat ttgcatatac
      421 tttgcagctc tggttggtgt taaaacttta gaaaatttct tataataact ttcattcaat
      481 tttacccgtg aagacttaaa atcaaatgca gctttcatca actggtctgc tttttcatct
      541 gtcatcttat catagtttgc agcataatct ttaatattct gaatgcgttt gtttgctaat
      601 ttttccatct caatttcaaa ctcattgtat actggccaaa aagcttttga ttcagcttct
      661 gtaaaagcca tagattctgt aataatagct tttctgtttg tcttaacatc tgacttcaga
      721 agttctatca tatcatctgc tgtttgtgcg tgtattgcaa aagtgccgag taaaaaaata
      781 gcacttaata ataatttttt catttctgtg ttccctttgt ttaagttatt taataatata
      841 aaatagattc ccgcctgcct gccgtcaggc aagctttcac gggaatgacg ttaaaaaaac
      901 atttctaaaa ccatttgctg gagaatgaga atccaagatt tatttcataa aaagaataaa
      961 aattgttctc actgccattt aaagcagttc cggaatcaaa tgcataatta tatcttgctc
     1021 caaaataaaa tgatgtatta tcaccatcaa atggaattat taggccggct tccggtgcta
     1081 atccaaagtg ccagttatca ttatcaattc tataaacgcc aatatcaaga cgctgaagta
     1141 tgtaatacat acctgcattc aatccgataa aagctctcat atcatctctt ttgccgtaat
     1201 gatgatgtaa tccgacaagc aaagggaatg aattaacgga acgtacttgt gtcccggata
     1261 aagttcctga tgcattttct ccacctgcta aatctattgg tgtattagtt ttctcgttga
     1321 acacattcca tcctgtcata aagccaacgg aattatattt atccaaaaat cttctgccgg
     1381 aaattgtaaa tccctgataa gttacatcag tagtatattg attcatttta gaaaccggta
     1441 taccgatatt ccaggtaaca ttggttagta attcctgtgc aaaacttgta aatgatatta
     1501 atacaagcag tgctgttata tattttattt tcataatatt ttattatctc caaatattta
     1561 tttaattaaa agttattcga tgatttttaa gtatggtgac tgttcaaaca ttttatctac
     1621 gctgtttata actcttgtta aaacatttgc atttgtatca tctagaattc catttacagc
     1681 acccgcccat actgcaccgg taaccttatt ggtttcatct attttggact tatcaatcat
     1741 tgttacaaat aaacttcctg ttgtataaga atatgcatat cctccatatc cataccaggg
     1801 atagtaccat ccgtaccatc cgtaccagta tcctggataa taaactatat tttcggatga
     1861 tgttgctcca acagtaacaa aaacatctgc ctcctgcgga gtgtttactc tggtgtatcc
     1921 ataagcaatc atctgtcttt caatttctgc aagtatttgt gcatcatatt gacccgttga
     1981 acctgatact gaaccattat tatctaaaat tctttttata gagtcaggca taaaaaaggt
     2041 tgccccatca gttttat
//
LOCUS       DASWBY010000779         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_32583, whole genome shotgun sequence.
ACCESSION   DASWBY010000779 DASWBY010000000
VERSION     DASWBY010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32583"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /locus_tag="VF870_14130"
     CDS             <1..1826
                     /locus_tag="VF870_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253380.1"
                     /translation="EESLLEQQKLAVSELDSKQRLYMERLEKLEDEINDQEQKCDSLK
                     SAIAQLTEEEGERRNRLEELSLALNLNQEQIDKKEIRLSALEKDEEEIKFRIENLTEK
                     LKEEEQKSEILKTEVERLTNENIQKKIKLEEISAEIDEKTASVTNRIIALENSENEKK
                     IALEELTNQIELQSAEAEKIRNTLSELNQTEKNTRLKVDELAEQIKEKVDRLNEIENS
                     FAVRENEAQLLESQMAEKLMEMNATNARQQKIFEAIEIKQKELFATEESLSIKTKRLA
                     KLSVEVTEIENRHKELSDETKRLEDLKNELHKRILLEQDSYDKLNKDKEKIKEILPLL
                     EQRREEIEKGNIDLENRFTKMFQRFNSEMNEITKKRSVIDQIVQKKEKDIEEKDQILF
                     EKINAIEESDRLLNMRQAEIDSFEQLLKTFNEKKEQLRSDLLKLDEQILEKKNSNDDM
                     KLEAELILKKRTSVEQSLQELINNMNQKFVEAKEKRSKVEKEARIYEEKVEEINHKIS
                     ESMDELVELQSAISMIKVEHEEHRGNIVKLASMKKKLQEEIAKSQRVLQRYQKIREKL
                     KIEQSILKNRQDSISRKNSIDDSADTSDNQQEQQTTRLFKI"
     gene            1908..3092
                     /locus_tag="VF870_14135"
     CDS             1908..3092
                     /locus_tag="VF870_14135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX9253381.1"
                     /translation="MPSVKLKKGRDKSFNRKHPWIFSGAVDSVKDINTNGETVTIISG
                     DGKILGYGSYSSHSQISVRVLSFNPDEKIDKNLFQNRIENAALLRKQIIDAQTNAFRL
                     INAESDLLPGLIVDKYADYLVCQFLSAGAEFWKKDIVEILINQFNPTGIFERSDVAVR
                     EKEGLQESKGILYGKQPEEFIEVIENGKKFLVDINLGHKTGFYLDQRDNRKIIENYSS
                     GCEVLNCFSYTGSFSVYALKAGAGKVVNVDSSAEALTLSDKNFALNGIDSSRYENIQD
                     DVFKYLRKQRDANKQFDVIILDPPKFAESVSQVEKASRGYKDINLLALKLLKKNGVLF
                     TFSCSGHIVSDLFNKIIADAAADAGREVHILKYLTQAPDHTMLTSFPEGLYLKGLVCK
                     AN"
     gene            3363..3719
                     /locus_tag="VF870_14140"
     CDS             3363..3719
                     /locus_tag="VF870_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type lectin domain-containing protein"
                     /protein_id="HEX9253382.1"
                     /translation="MKKLSVLAVVLFFVAFSALASAQTQSGQWSTKAGDAGYNLDTNT
                     GERAMTIEVNFKTPYEVKPKVMLSVTQIDADKGSNSRYNVEVISVSRDGFTVKIRTWA
                     DSKVYSISGFWLAYVE"
     gene            3831..4052
                     /locus_tag="VF870_14145"
     CDS             3831..4052
                     /locus_tag="VF870_14145"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEX9253383.1"
                     /translation="MNFKNISLLVLLALFIIVCIQNVEIISVQFLFWKAEISKLLLLI
                     ITWIIGLLIGMIIPGFLTKSKKEDKTKDK"
     gene            complement(4097..4297)
                     /locus_tag="VF870_14150"
     CDS             complement(4097..4297)
                     /locus_tag="VF870_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX9253384.1"
                     /translation="MKTNVGSADRIIRLVLGVVIIALGFYFKSWWGVVGLVPLGTGLI
                     NFCPVYSLIGISTKKKIQPEKL"
     gene            complement(4447..>4918)
                     /locus_tag="VF870_14155"
     CDS             complement(4447..>4918)
                     /locus_tag="VF870_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9253385.1"
                     /translation="HHRSDAITSGAAFIGIAIALIGGRGYEQADDYAALCASAIIIFN
                     AYRLFKPALFEIMDTAPSPEVIKKVIETSLKVDGVVAIDKCFARKMGFEYYIDMHVIV
                     NGDISVHNGHEISHKVKDTLVESFPNISDVLIHIEPATDERLKRKHIIEQTKRK"
BASE COUNT         1898 a          686 c          886 g         1448 t
ORIGIN      
        1 atgaggaatc attattagag cagcaaaaac ttgcagtaag tgaactggat tccaagcaaa
       61 gattatatat ggaaaggctt gagaaactgg aagatgaaat aaatgatcag gaacagaaat
      121 gtgattcatt aaaatcagct attgcacaac ttactgaaga agagggcgag agaagaaacc
      181 gattagagga attatccctt gcattgaatt taaatcagga acagattgat aaaaaagaaa
      241 ttagattatc agcgcttgaa aaagatgaag aggaaataaa atttagaatt gaaaatctga
      301 cagaaaagct taaagaagaa gaacaaaaat ctgaaattct aaaaactgaa gttgaaagac
      361 ttacaaatga aaacattcag aaaaagatta agcttgaaga aataagtgca gaaatagatg
      421 aaaagacagc cagcgtaaca aatagaataa tagctcttga aaacagtgag aacgagaaaa
      481 aaattgcttt agaagaactt actaatcaaa ttgaactaca aagcgcggaa gctgaaaaaa
      541 ttcgtaatac actatccgag ctcaatcaaa cggagaaaaa tacacgatta aaagtagatg
      601 aacttgctga gcagattaaa gaaaaagttg atcggcttaa cgagattgaa aacagttttg
      661 ctgtgcgtga aaatgaagct cagttattag aatctcaaat ggctgaaaaa ttaatggaga
      721 tgaatgcgac aaatgcccgg cagcaaaaaa tatttgaggc aatcgaaatc aagcagaagg
      781 aactttttgc tactgaggaa tcactaagta ttaaaaccaa aagattggct aagctcagcg
      841 tagaagtaac ggaaattgaa aaccggcata aagaactttc tgatgaaaca aaacgacttg
      901 aggatcttaa gaacgagttg cacaaaagga tattgcttga gcaggattcc tatgataaac
      961 tgaataagga taaagaaaag ataaaagaaa tactgcctct tttggaacaa cgacgcgaag
     1021 aaattgaaaa gggcaatatt gatcttgaaa acaggttcac aaaaatgttt cagcgattta
     1081 attctgagat gaatgagata acaaaaaaga gatctgttat tgatcaaatc gttcagaaaa
     1141 aagaaaagga cattgaagaa aaagatcaga tattatttga gaagattaac gcaattgaag
     1201 aaagcgatcg tttattaaat atgcgtcagg ctgagattga ttcttttgaa cagctgttaa
     1261 aaacatttaa cgaaaagaaa gaacagctta gaagtgattt attaaaactt gacgagcaaa
     1321 ttttagaaaa gaaaaattcc aatgatgata tgaagctgga agcagaactt attttaaaga
     1381 agagaacttc tgttgaacaa agtttgcagg aactaattaa caacatgaat caaaaatttg
     1441 tcgaggctaa agaaaagcga tcaaaggttg aaaaagaagc aagaatctat gaagaaaaag
     1501 ttgaagaaat taatcataag atttcagaat caatggatga acttgttgaa ttacaatcag
     1561 caattagtat gattaaagtt gagcacgaag aacatcgcgg caatattgtt aagcttgctt
     1621 cgatgaagaa gaagctgcag gaagaaatcg caaagagtca aagagtactg cagcgttatc
     1681 agaagataag agaaaagttg aaaatcgagc aatctatttt aaaaaacagg caggattcca
     1741 taagcagaaa aaattctatc gatgactctg cagatacaag cgataatcaa caggaacaac
     1801 agactacaag attgtttaaa atttagaagc acagctattt tagatttcaa gggatcattt
     1861 aattgatccc ttttttgttt taactaaata aattagagtt gtacaccatg ccgtcagtaa
     1921 aattaaaaaa gggacgagat aaatctttta acagaaaaca tccctggatt ttttcaggag
     1981 cagttgattc tgtaaaagat ataaacacaa atggagaaac agttaccatt atttctggtg
     2041 atggaaaaat acttggatat ggttcttatt cttcacattc acaaatatct gtgagggttt
     2101 tatcatttaa ccctgatgaa aagattgata aaaatttatt tcagaatcga attgaaaacg
     2161 ctgcactatt acgcaagcaa ataattgatg cacaaaccaa tgcctttcgt ttaattaatg
     2221 cggaaagcga tcttttacca ggacttatcg tagataaata tgctgattat cttgtctgcc
     2281 aatttttatc tgcaggagct gaattttgga aaaaagatat agttgaaatt cttataaatc
     2341 agttcaatcc cacaggaata tttgaacgct ctgatgtagc ggtaagggaa aaagaaggat
     2401 tacaggaatc gaaaggaatt ctttatggta aacaacctga agagtttatt gaagttattg
     2461 aaaatggaaa aaaattttta gttgatataa atctcgggca taaaacagga ttctatcttg
     2521 atcagaggga caatagaaaa attatagaaa actattcatc cggttgtgaa gtgcttaatt
     2581 gtttttctta cacaggcagt ttttctgttt atgcacttaa agcgggtgcc ggcaaagttg
     2641 tgaatgttga ttcctctgca gaggcactga ctttatctga taaaaatttt gcgctaaatg
     2701 gaattgattc atccaggtat gagaacattc aagatgatgt ttttaaatat ttaagaaagc
     2761 aacgggatgc aaataaacaa tttgatgtca ttattcttga tcctcctaaa tttgccgaat
     2821 cagtaagcca ggttgaaaag gcaagtcgag gatataaaga tattaatctc ttggcgttaa
     2881 aacttttgaa gaaaaatggg gtactattta ctttttcctg ctcgggtcat attgtttcag
     2941 atctttttaa taaaattatt gctgatgcag cagcagatgc tggtagagaa gttcatatat
     3001 taaagtattt aactcaagct ccagatcaca caatgctgac aagctttcct gaaggacttt
     3061 atctaaaagg attagtttgt aaagcgaact gatcacaaaa atattattat aatttatgag
     3121 atggatataa atgatatttc tgagtgtttt gttgagtctg ctgatttgat tatatgctga
     3181 ttacagcaaa cttaaatggg tcaataattg ccgaacttag aacgtcagaa ataatataat
     3241 tatcagaata acctattagg aaataaaaaa tcaaacgatt aaattttatt aaagatttat
     3301 actaaatatt aagaaaacat ctaattactt taattactca ataacaaatc aaggagataa
     3361 atatgaaaaa actatcagtt ctagcagttg tattattctt tgtcgctttt tctgctttgg
     3421 cctcagctca aactcaaagt ggtcaatggt caacaaaagc aggtgatgct ggttataact
     3481 tagatacaaa tactggtgaa agagctatga caatcgaagt aaacttcaaa acaccttacg
     3541 aagtaaagcc aaaagttatg ttgtcagtta cacagattga tgctgataaa ggatctaata
     3601 gcagatataa tgttgaagtt atttctgtat caagagatgg atttacagta aaaatccgta
     3661 cctgggcaga ttcaaaagtt tatagcatca gtggtttctg gttagcttac gtagaataat
     3721 aatttctgtt tcattttttt atatgccata tctaaataag atatggcatt tattttctta
     3781 agataaacga aatattattt tgaatttatt ttatgattac ggagaacaga atgaatttta
     3841 aaaatataag tctattagtt ttgcttgcac tatttattat agtttgtatt caaaatgtcg
     3901 aaatcatttc tgttcaattt ttattttgga aagcagagat atcaaaactt cttcttttaa
     3961 taatcacttg gataattggg ctattaattg gaatgattat tccgggattt ttgactaaat
     4021 ctaaaaaaga ggacaaaaca aaggataaat aaaaagggta ttaaaaccct ttttatcttt
     4081 aaaataaatt aaacttttag agtttttcag gttgtatttt tttctttgtg gaaataccga
     4141 ttaaactata aacaggacag aaatttatta atccagtacc aagaggtact aaacctacaa
     4201 ctccccacca gcttttaaaa taaaagccaa gagctataat taccactcct aaaactaatc
     4261 gaataatacg atctgcactt ccaacatttg ttttcataag aatctccatt aaggttatta
     4321 attataaaat agaataaaaa tttaattctt gtgtataaga tgctcagtga atgacaagga
     4381 tgcaagaagt caaattatat gacggaaagg gccattatct accgaaagga ataagaaaat
     4441 caaattttat ttcctttttg tttgctcaat aatgtgtttt ctctttaatc tttcgtctgt
     4501 ggcaggttct atatggatta gaacatccga aatgtttgga aatgactcaa ctaatgtatc
     4561 ttttacttta tgagatattt catgtccatt atgaacggat atgtctccat taacaattac
     4621 gtgcatatct atataatatt caaatcccat ttttctagca aaacacttat caattgctac
     4681 aacgccatca actttaagag aagtttcaat aactttcttt atcacttcag gtgatggtgc
     4741 ggtatccata atttcaaata atgcgggttt gaatagtcta taagcattaa agatgataat
     4801 cgcggaagcg cacaatgcag catagtcatc cgcttgttca taaccacgac cgccaatcag
     4861 tgcaatagca atacctataa aagcggctcc agaagtaata gcatcacttc tatgatgc
//
LOCUS       DASWBY010000780         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_107235, whole genome shotgun sequence.
ACCESSION   DASWBY010000780 DASWBY010000000
VERSION     DASWBY010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107235"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..887
                     /locus_tag="VF870_14160"
     CDS             147..887
                     /locus_tag="VF870_14160"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX9253386.1"
                     /translation="MKKFLIALGILFALILVLNYILLPLYVSGSEINVPNVVGMTEEE
                     AFKTLKDEDFTPSIADTSFGVSIPPGRVFLQKPESGKIVKEGRTVYLFISGGEQLISV
                     PLLKGKSVRDARLALERIGLKLGSVEELASTNPKDMVFDQQFAEGTNIRKGQSVGVSV
                     SIGKGDGEIEVPDLIGKSLTEATRILSDSTLTVGKVNYQISSTLLPNTVLDQYPAPGN
                     KLNSGDKVDLFITKEGTIKERSEFPSEN"
     gene            888..1544
                     /gene="rpe"
                     /locus_tag="VF870_14165"
     CDS             888..1544
                     /gene="rpe"
                     /locus_tag="VF870_14165"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX9253387.1"
                     /translation="MKLLAPSILSADLSNLAQQIRLVEMSGADWIHCDIMDGHFVPNI
                     TFGPVVVKAAKKCAKLPLDVHLMIKNPDIFLEDFRNAGADIISVHFEEVVHLNRTVNR
                     IKELGAKAGVVINPSTPVASLKDIVEYIDLLLIMSVNPGFGGQSFISNTERRIAEAVE
                     LRKSLNAKFLIEVDGGLNNKTIKSVLKAGCDVFVAGSSIFHNDNISAATAELKNLISN
                     "
     gene            1642..2472
                     /gene="folP"
                     /locus_tag="VF870_14170"
     CDS             1642..2472
                     /gene="folP"
                     /locus_tag="VF870_14170"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9253388.1"
                     /translation="MFDLKYNFKNKPLSFNSPKIMGIVNVTPDSFSDGGKYFSVDAAV
                     DHGLKLLDEGADIIDIGGESTRPGSDPVPVEEELRRTIPVIKNILKQRKDSIISIDTT
                     KSEVANRALDTGAELINDISGLTFDENMLLVAKEFDAGIVIMHIKGNPKTMQQNPEYK
                     DVVKEVYDFLLVQSLQAKQNNLDKIIVDPGIGFGKRIEDNFNLIKNLDYFKSLGYPVM
                     IGLSKKSFIGKTLNLDIDQREIGTVILETISILKSARIIRTHNVKYCNQIVKLASHIL
                     "
     gene            2479..3297
                     /gene="cdaA"
                     /locus_tag="VF870_14175"
     CDS             2479..3297
                     /gene="cdaA"
                     /locus_tag="VF870_14175"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX9253389.1"
                     /translation="MIDLFKIGFLTITLSDLIDIAIVSFIFYKLYTILRGTIAAQIFI
                     GLLVIILVSFIAQTLNFRAVSWLLRLITDIWVIAFIILFQPEIRRLLVLLARNPFFKV
                     FAKAESHEVADLIADAAFQLSQNQHGALIVLIRSAGIRGFAETGEILNARLSASLLTS
                     IFYPRSPLHDGAVIVNKNFIEAARCTLPLSQQTSIKGESLGMRHRAGLGISEQADVLS
                     IIVSEETGSISIAENGELTKGLSKDALKRILNKALRGPEKKGWKSFIDQLKSKE"
     gene            3272..3940
                     /locus_tag="VF870_14180"
     CDS             3272..3940
                     /locus_tag="VF870_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9253390.1"
                     /translation="MTSLNQKNKVCAVIPFYNEKDFLFDVVSQTLNFVDHIIAVNDGS
                     TDGSEKTVNNLERVQVISLNRNYGKGYALQIGFNESIKKNFTYTITLDADKQHNPAFI
                     PKFISQLNNFDIVIGNRLNDTKIMPIQRILSNKITSQLLSIKTGQRILDSQCGYRAYK
                     NEVLNKVKSISFGYEAESEIIIYSAREGFKIGSVNISTIYGNEKSKMNPVKAILGFIR
                     ILLK"
BASE COUNT         1400 a          580 c          725 g         1264 t
ORIGIN      
        1 ttttgtacat aaataaatct ctttattact tgcaatctaa ttattttaac tataaaagtt
       61 gggcactcaa ctgcaaatgt agtttgttaa aaatttgcac tattgatata tttgaatcaa
      121 tgaaaaagct actccaatat cgaatggtga aaaagtttct aatcgcttta ggaatacttt
      181 tcgccttaat ccttgtactc aattatattt tattgccatt gtacgtaagc ggatcagaaa
      241 ttaatgttcc caatgtcgta ggaatgactg aagaagaagc atttaaaact ttaaaggatg
      301 aggactttac accttcaatt gcagatacat ccttcggagt ttcaattccg ccaggtaggg
      361 tatttttaca aaagcctgaa tcaggaaaaa tcgttaaaga gggaagaaca gtatacttgt
      421 ttattagcgg cggtgagcag cttatttcgg tccctctatt aaaaggaaaa tccgttagag
      481 atgcaagatt agccttagaa agaattgggt taaaacttgg ttctgttgaa gaattggcct
      541 caacaaaccc taaagatatg gtctttgatc aacaatttgc agaaggaacc aatataagaa
      601 aaggacagag tgttggagtt tctgttagta ttggtaaagg agacggtgaa attgaggttc
      661 cggacttaat aggaaaatct ctaaccgaag caacaagaat tttatcagac agcacactta
      721 cagttggaaa agttaattat cagatctcaa gtacattact tccaaacaca gttcttgacc
      781 aatatcctgc accgggcaac aaattaaatt ccggtgataa agtcgatctt tttattacca
      841 aagaaggaac cattaaggaa agatcagaat ttccttcgga gaattaaatg aaactcttag
      901 caccgtcaat attatcagca gacctctcaa atcttgcgca gcaaatccga ttagtagaaa
      961 tgagtggggc tgattggatt cattgtgaca taatggatgg acactttgta ccgaatatta
     1021 ctttcggacc ggttgttgtt aaagctgcaa aaaaatgcgc aaaacttcca cttgatgttc
     1081 atttgatgat taaaaaccca gacatttttc ttgaggattt tcgcaatgcc ggtgctgata
     1141 ttatttctgt acactttgaa gaagttgttc atcttaacag gacagtaaat agaattaaag
     1201 aactgggtgc aaaagctggt gtagtaatta atccatccac tccagttgca tcattaaaag
     1261 atatagtcga gtatattgat ttattattaa ttatgagtgt taatcccgga tttggcgggc
     1321 agagttttat ttctaataca gaacggagaa ttgctgaagc ggttgaatta agaaaatcac
     1381 taaatgcaaa atttttaatt gaagtagatg gtggattaaa caacaaaaca ataaaatcag
     1441 tattaaaagc cggttgtgat gtatttgtag cgggatcgtc catttttcat aatgataata
     1501 tttctgctgc cactgctgag ctaaagaacc ttatttcaaa ttagaatttt gtctctttaa
     1561 attttaataa agcggcttat aaccgctttt tttatttaga aatgttttaa gtttagcgta
     1621 agattattta aataacagaa aatgtttgat cttaaatata attttaaaaa taaaccatta
     1681 agctttaact ctcccaagat aatgggaatt gttaatgtta caccagattc attttcagat
     1741 ggcggaaaat acttttctgt tgatgctgca gttgatcacg gattaaaatt attagatgaa
     1801 ggagcagata tcattgatat cggaggagaa tcaactcgtc cgggttctga tccggttcca
     1861 gttgaagaag aattaagaag aacaatcccg gttataaaaa acattctcaa acaaagaaaa
     1921 gattcgataa tttctatcga tacaacaaaa agtgaagttg ctaatcgggc acttgatacc
     1981 ggtgctgaat taataaatga tataagcggt ttaacatttg atgagaacat gctgctcgtt
     2041 gcaaaagaat ttgatgcagg aattgtaata atgcacatta agggtaaccc aaaaacgatg
     2101 cagcaaaatc ctgagtacaa agacgttgta aaagaagttt atgatttttt attagtgcaa
     2161 agcctacaag caaagcaaaa taatttagat aaaataatcg tggatccagg tataggattt
     2221 gggaaacgta ttgaagacaa ttttaatttg atcaaaaatt tagattactt caaatcactc
     2281 ggttatccgg tgatgattgg attatcaaaa aaatccttta taggtaaaac tttaaatctt
     2341 gatattgacc aaagagagat tggtacagta atattggaga caatatctat attaaaatca
     2401 gctagaataa ttcgcactca taatgtaaaa tattgcaatc aaatagttaa attagcttca
     2461 cacattcttt aatatttaat gattgatctt tttaaaatag gatttctgac aataacatta
     2521 tccgatctaa ttgatattgc tattgtttcg tttatctttt acaaactcta tacaattctc
     2581 cgcggaacaa tagctgcaca aattttcatc ggattacttg taattatttt agtttcattt
     2641 atagctcaga ctctaaactt tagagcggta agctggttat taaggctgat aactgatatc
     2701 tgggtaatag ctttcataat tttgtttcaa cctgaaatca gaaggctatt agttttatta
     2761 gcgcgtaatc cgttctttaa agtatttgct aaagcggaat cgcacgaagt tgctgattta
     2821 attgcagatg cggcctttca attatctcaa aatcaacatg gggcattaat tgtattaata
     2881 agatcagccg gaataagagg ctttgctgaa accggtgaaa tattaaatgc ccgattaagt
     2941 gcttcgctct taacttcaat attttatcca aggtctccgt tacacgatgg agctgtaatt
     3001 gttaataaaa attttataga agctgcaaga tgcacacttc cgctatcaca acagacttcc
     3061 ataaaaggtg aaagtttagg aatgaggcat cgtgctggat taggaatctc tgaacaggct
     3121 gatgttttaa gcattatagt ctctgaagaa accggcagta tatcaatagc agagaatggt
     3181 gagctaacaa aagggctgtc taaagatgca ttgaaacgta tattaaacaa agcattaagg
     3241 ggacctgaaa aaaaaggatg gaaaagtttt attgaccagc ttaaatcaaa agaataaagt
     3301 ttgtgcagta attccttttt ataatgagaa agatttttta tttgatgttg tttctcaaac
     3361 tttaaatttt gttgatcaca taattgctgt aaatgatggc tcgactgatg gttcagaaaa
     3421 aactgttaat aatttggagc gggtgcaggt aatttcgtta aatcgtaatt atggtaaagg
     3481 atatgcgctg caaattggat ttaatgaaag tataaaaaaa aacttcactt atacaattac
     3541 tcttgatgct gataaacagc ataatcctgc attcattccc aagtttattt ctcaacttaa
     3601 taattttgat attgtgattg gtaacagatt aaatgacact aagattatgc caatccagag
     3661 aatactcagt aataaaataa cgtcacaatt gcttagtatt aaaaccggac aaagaatttt
     3721 ggatagccag tgcggctatc gtgcctataa aaatgaagtt ttaaataaag taaaatcaat
     3781 ttcttttgga tatgaagctg aaagtgaaat aattatttat tctgctcgcg aaggttttaa
     3841 gattggatct gtgaatattt ctacaattta tgggaatgaa aaaagtaaaa tgaatcctgt
     3901 aaaagctata cttggtttta taagaatatt attaaaataa agattattat gaaacgcaaa
     3961 tgggaaatt
//
LOCUS       DASWBY010000781         5101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_32658, whole genome shotgun sequence.
ACCESSION   DASWBY010000781 DASWBY010000000
VERSION     DASWBY010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32658"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VF870_14185"
     CDS             complement(<1..1002)
                     /locus_tag="VF870_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX9253391.1"
                     /translation="MENIILFPFADYWLFYVGFTAFVLMMLALDLGVFHRDAHEVTFK
                     ESLTWSIIWISLALIFNFLFFKYAGWKFENDARLLAIPGFDGNAAANQVGLEFLTGYI
                     VEKTLAIDNIFVFVVVFSFFAVPLKYQHRVLFFGILGALIFRIIFIAMGSILLQYQWI
                     VIFFGAFLILTGFKIIFAPEKPIDPEKNPVIKLFKKFFPVTSTFEGQKFFIRKEGVLY
                     ATPLMVALLFIEVSDIIFAVDSVPAIFAITKEPLIVFTSNVFAILGLRAMYFMLAGVI
                     HKFRYLKYGLGIVLVFVGLKMAWLNEAFGGKFPISWSLIIIVTIIGSSILISLFKKQK
                     "
     gene            complement(1133..1606)
                     /locus_tag="VF870_14190"
     CDS             complement(1133..1606)
                     /locus_tag="VF870_14190"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX9253392.1"
                     /translation="MKISHIIKLIISILLPLSVGAIAGMFTSQAVPFWYASLNRPSFS
                     PPNWVFGPVWTSLYILLGISFFLIWKEEASKERDLAIKVFSIQMLLNFAWSFIFFYFN
                     LIGAALIEIILLWTSIAAMIYLFYKIKPVAAYMNIPYLLWVSFATILNAGYYFLN"
     gene            complement(1692..1934)
                     /locus_tag="VF870_14195"
     CDS             complement(1692..1934)
                     /locus_tag="VF870_14195"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX9253393.1"
                     /translation="MRNIIIKTQKQAKRLIIIVVGFTVLLLGVAMILLPGPAFIMIPL
                     GLGILATEFVWARTLLNKFKEKLGRITKTNFTDKTK"
     gene            complement(2017..2766)
                     /locus_tag="VF870_14200"
     CDS             complement(2017..2766)
                     /locus_tag="VF870_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253394.1"
                     /translation="MKNRFSFAVTLILLISSFLTASLNAQVILDFETGLVSTGYNDVR
                     IPNNQGTLISFKDDLTPSSGVFYRIRLNYTFSSAHTLSLLFAPLTVKSEGRINKDVNF
                     AGSVFPANQDLIGSYKFNSYRLTYRYDFVQNPTLEFGLGFTAKIRDARIALSSPEISA
                     EKSNVGFVPIINFRLFWHSYEKLGFLLEGDALAAKQGRAIDVLFAASYKLSNQLGIKA
                     GYRILEGGADNDEVYNFALFHYLSFTFSYTF"
     gene            complement(2852..3664)
                     /locus_tag="VF870_14205"
     CDS             complement(2852..3664)
                     /locus_tag="VF870_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX9253395.1"
                     /translation="MNREKIKQFIESQSIQNFIIGLIVLNSITIGFETSDKVMSSIGD
                     TLLLIDKIILAIFVLEITLKLYAYGFSFFKGGWNVFDFSIVVIALLPASGVLAVLRSL
                     RIFRSLRLIKNVPKLRFIVESLFHSLPSLVWIFVLLALVFYVFSVIGTKLFGADYPQW
                     FGTLAASMFTLFQIMTLEGWAEISREIMEKYPFANIYFILFILLASYTTLNIFIAIVV
                     NTMAEVQQKVSVEEVNKIGTIIQDENEDLKNDIKLLKEQIIKMEEKLTKRVN"
     gene            complement(3702..4259)
                     /locus_tag="VF870_14210"
     CDS             complement(3702..4259)
                     /locus_tag="VF870_14210"
                     /inference="COORDINATES: protein motif:HMM:NF020136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HEX9253396.1"
                     /translation="MNLPAIILSAILMITSIKQNQDGYLLNSSIMKTENKWRIVNDGV
                     MGGLSSSKAIVNDDNKIIFSGNVSLENNGGFASLRSAVKDYNFENFSGLEIKIIGDGK
                     RYSISMKETTYFSGYFYTSTFETKKDEWITIKIPFDQLKLYYFGKDAKSNKIIPLNNI
                     KEISLLVGDKQEGSFKVEIDYIKLY"
     gene            complement(4351..>5101)
                     /locus_tag="VF870_14215"
     CDS             complement(4351..>5101)
                     /locus_tag="VF870_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253397.1"
                     /translation="DWAKQQGIDLPLPFGIAANYIFMSRDVDVTDVTVQFLNRAPESI
                     NDFTSFAVRNNTSVAAVKFDAWILPLLNVYVSVGYASTIAKMNANITIERSILPLPPV
                     ELDIETETKIADLYWGIGSTLVAGYKSWFIMADANYGATSPDELNNSIDFTFLSARSG
                     FMGKIGQDNSMKAWLGAAYLFSTTTLEITVPSGSSLGDVLVKIEQQPVNPWTFHSGFM
                     ISLNKRFDFMTELGTNFKDASIAVLSATFRF"
BASE COUNT         1813 a          897 c          790 g         1601 t
ORIGIN      
        1 tttttgtttt ttaaataacg aaattaaaat tgatgaaccg ataatagtta caattattat
       61 tagagaccag ctaataggaa actttccacc aaatgcttca tttaaccacg ccatctttaa
      121 tccaacaaac acaagcacga ttccaagtcc atattttagg tagcggaatt tatgaattac
      181 cccggcaagc ataaaataca ttgcacgtaa gccaagaatt gcaaaaacgt tggaagtaaa
      241 aacaattagt ggttcttttg tgatagcaaa gattgcagga acagagtcaa ctgcaaaaat
      301 aatatctgat acttcgataa aaagcagcgc aaccatcaat ggagtagcat aaagcactcc
      361 ttcttttcgt ataaagaatt tttgtccttc aaaagtggat gtaacgggaa agaacttttt
      421 aaacaatttt ataacgggat ttttctccgg atcaatcggt ttttcaggcg caaagattat
      481 cttaaaacct gttagaataa gaaacgcacc aaagaaaata acgatccatt gatactgaag
      541 cagaatggaa cccatagcaa taaatattat acgaaatatt agtgctccga gaatcccaaa
      601 gaataaaact ctgtgctgat attttaacgg tacagcaaaa aaactaaata caaccacaaa
      661 aacaaaaatg ttatctattg ctaaagtttt ctcaactata tacccggtta aaaattccag
      721 tccgacttga ttggctgcgg cattgccatc aaaaccagga attgcaagaa gccgtgcatc
      781 gttttcaaac ttccagccgg catatttaaa gaaaagaaaa ttgaaaatga gcgcgagaga
      841 aatccaaata atactccacg ttaatgattc cttaaaagtt acttcgtgtg catctctatg
      901 aaatacaccc aaatctaaag ccagcatcat taaaacaaat gctgtaaatc caacataaaa
      961 caaccagtaa tctgcaaaag gaaacaggat aatgttttcc aaggtttaac tctctcctat
     1021 cataatctat ttattaaaaa aactcaacgg taaacgaatt taataagccc agtgcaataa
     1081 ataccaaatt ctttcggatg gttttattta taaaattacc ttcatggatc ttttaattta
     1141 agaaataata tccggcatta agaatagtag caaagcttac ccagagcaaa tatggaatat
     1201 tcatataagc tgcaaccggt ttaattttat agaacagata aatcattgca gcaatgctag
     1261 tccataaaag aatgatttct ataagcgcag caccaatcaa attgaaatag aagaatataa
     1321 aactccatgc aaagtttaag agcatttgga ttgaaaatac cttgatagcc aggtctctct
     1381 ctttcgatgc ttcctctttc caaataagaa aaaatgatat tcctaaaagg atatatagag
     1441 aagtccatac cggaccgaaa acccagttag gaggactgaa tgaaggtcta tttaatgaag
     1501 cataccaaaa aggtacagcc tgagaagtaa acatacctgc tattgctcca acggataatg
     1561 gtaataaaat ggatataatc agtttaataa tgtgagatat tttcataact atttttgtct
     1621 tgagttaatt ttatccggat taataaaaac aaagactttt gtgtaaaaca ctgcatctga
     1681 tatttaccac attactttgt tttatcagta aaattagttt tagtaattct gcctaatttt
     1741 tctttaaact tgtttaacag tgttcttgcc caaacaaatt ctgttgcaag aatgcctaag
     1801 ccaagcggta tcataatgaa tgctggtcca ggcagtagta tcatagctac tcctaaaaga
     1861 agtactgtaa aacccactac tattatgatt aaccttttag cttgcttttg agttttaata
     1921 ataatgttcc gcactaatta aaattctcct aactcaattg taaaggttta attttaaaat
     1981 acctgtaaag attatttgtt gtaaattcat aaaaaattaa aaagtatatg aaaaagtaaa
     2041 agacagataa tggaaaagag caaaattgta aacttcatca ttatctgcac ctccttctag
     2101 aatacgatac ccagctttaa ttccaagctg attggataat ttgtatgatg cggcgaaaag
     2161 aacatcaatt gctcttccct gtttagccgc gagagcatct ccttctaaaa gaaagccaag
     2221 cttttcatat gaatgccaga ataatctgaa attaataatt gggacaaaac caacattgga
     2281 tttctcagca gatatttctg gagatgaaag ggcaattctt gcatctctaa ttttagcagt
     2341 aaatcctaaa ccaaattcaa gggtaggatt ttgcacaaaa tcgtaacggt atgttagtct
     2401 gtaggaatta aatttatatg acccgattaa gtcttgattt gcaggaaata cactgccggc
     2461 aaagttaaca tctttattta ttcgtccttc agatttaaca gttaatggcg caaataataa
     2521 agataaagta tgggcagaac taaaagtgta attgagtctt atcctataaa aaacaccgga
     2581 acttggcgtc agatcatctt taaaggagat aagtgtgcct tgattattag gaatgcgcac
     2641 atcgttataa ccggtgctta caaggccagt ttcaaaatcc aaaataactt gggcatttaa
     2701 tgatgctgtt agaaaagagc ttatcaataa aattaatgtc acagcaaatg aaaatctatt
     2761 tttcattttt tataaatctg atttagttat cagcaaaatc tttaatagca ccataaaaca
     2821 ttttcttgat tgtagtataa aatatgatac atcaatttac tcttttcgtc aatttctctt
     2881 ccattttaat tatctgctct tttaaaagtt taatatcgtt tttcaagtct tcattttcat
     2941 cctgtataat cgttccaatt ttattaacct cttccacact aactttttgt tgaacctcag
     3001 ccatagtatt tacaacgatg gcaataaaaa tattcagtgt ggtatatgat gcaagaagta
     3061 taaagagaat aaaataaata tttgcaaacg ggtatttttc cataatttct ctcgaaatct
     3121 ctgcccatcc ttcaagtgtc attatctgaa ataatgtaaa cattgatgca gcgagtgtgc
     3181 caaaccattg aggatagtca gcaccaaaaa gctttgtccc aattactgaa aaaacataaa
     3241 atactaatgc cagcagaaca aagatccaaa ctaggcttgg gagcgaatga aataatgatt
     3301 caacaatgaa tcttagctta ggtacatttt tgattaatct aagtgaacgg aaaattctta
     3361 gagaccgcaa aaccgctaaa actccggaag ctggcagtaa agcaattacc actatcgaaa
     3421 aatcaaaaac attccagccg cctttaaaaa atgaaaatcc atacgcatat aactttaaag
     3481 ttatttctaa aacaaaaatt gcaagaataa ttttatcgat taaaagaagc gtgtccccaa
     3541 ttgaagacat tactttgtca gatgtttcaa atccaattgt aattgaattt aaaacaatca
     3601 atccgataat aaaattctgt atggattggc tttctatgaa ctgtttaatt ttttcccgat
     3661 tcatttctac aataattatt atttaacata tattaattta atcagtaaag ttttatatag
     3721 tcaatttcca ctttgaaaga accttcttgc ttatctccaa ccaataagga aatttctttg
     3781 atattgttaa gtggaattat tttatttgat ttagcatctt ttccaaagta gtaaagcttc
     3841 aattgatcaa aaggaatttt gattgtaatc cattcatctt tctttgtttc aaaagttgag
     3901 gtgtagaagt agccggaaaa gtaagtcgtc tctttcattg aaatgctgta acgctttcca
     3961 tcgcctatga tttttatttc taatccggaa aagttttcaa agttgtaatc ttttactgca
     4021 cttctgagcg aagcaaatcc gccgttattt tctaaagaaa catttccact gaagataatt
     4081 ttattatcat cattaactat tgccttagag gaacttaatc cgcccattac accatcgtta
     4141 acgatacgcc atttgttctc ggttttcatt attgatgagt tgagaagata accatcctga
     4201 ttttgtttta ttgatgtaat cataagaatt gctgataata taattgctgg taaattcatt
     4261 ttattgcttt atagttttag tgttttaatt aactatagaa caataataaa tattaattcg
     4321 ttcaatctaa tttttgaaaa acaaaccaaa ttaaaaacga aacgttgcgc ttaagacagc
     4381 aatagaagca tccttaaaat ttgttcccaa ctcagtcata aaatcaaatc gtttattcag
     4441 gctgatcata aatccggagt ggaatgtcca cgggtttacg ggctgctgtt cgatttttac
     4501 aagcacatct cctaagctag accctgaggg cactgtaatt tcgagagttg ttgtggagaa
     4561 tagatatgct gcaccgagcc aggctttcat agaattatcc tgacctattt ttcccataaa
     4621 accagagcgg gcagaaagaa atgtaaaatc aattgagttg tttaattcat ccggcgatgt
     4681 tgccccataa ttagcatctg ccataataaa ccagctttta tatccggcaa ccaaagttga
     4741 tccaattccc cagtagagat ctgctatttt cgtttctgtt tcaatgtcta actcaaccgg
     4801 aggaagagga agtatggatc tttcgattgt aatatttgca ttcattttcg caattgtgct
     4861 ggcatagcca acagacacat aaacatttaa taatggtaat atccaggcat caaatttaac
     4921 cgctgcaacg gaagtgttgt ttcttactgc aaaacttgtg aaatcattaa tgctttcagg
     4981 agcccgatta agaaactgaa cagtaacgtc tgttacgtca acatcgcgac tcataaaaat
     5041 ataatttgcg gcgattccaa atggtaaagg caaatcaatt ccttgctgct ttgcccaatc
     5101 a
//
LOCUS       DASWBY010000782         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_746969, whole genome shotgun sequence.
ACCESSION   DASWBY010000782 DASWBY010000000
VERSION     DASWBY010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746969"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1670
                     /locus_tag="VF870_14220"
     CDS             <1..1670
                     /locus_tag="VF870_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253398.1"
                     /translation="HNRNTSAKFIFSVAGRIDLIDGIVLEDFCEIDGSNQIELSGTAI
                     MTTQNNTKIKGLRISTMFGVELGGNNNIVGDFPYYNEFVNCYVGLTINGDNNKLFNNY
                     FGITQDGTLMPNQFGMIVSGSNNEIGKSDMANGNVVCGNSVVGIEIALGSGNKIQHNL
                     IGTTFDGRTDVGNGQGITIGGSDNNIIGGDNLVDGNVISGNSVYGIFISGAPPDSYSA
                     DNLIKNNNIGLNPDKTAAVPNARGITITNATMSAQIVDNIISGNTNEGIYIFGYDDET
                     FTNGHLIHGNSIGTNGAQGSFPNDGDGILIAGNVGTVKIGQDENGNYTGNTIIGNGSV
                     GVNIVPLLGYSPERITVRRNSLNQNLLTNLFVDTSANNRIREPYNLNYNAGLISGKHA
                     LPNAIIDIYSANKFELPASAYFRIGTTNTDANGNFSFSTNLNIEAIAVTATDFEGNTS
                     NFARLNIVTAVEDEKQIPAEFSLEQNYPNPFNPSTKISWQSPVGSHQTLKIYDVLGNE
                     VATLVDEYREAGSYEVEFNASLLPSGVYFYKLQEDGFVKTKKMTLIK"
     gene            1697..>1990
                     /locus_tag="VF870_14225"
     CDS             1697..>1990
                     /locus_tag="VF870_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253399.1"
                     /translation="MKKISTIVLILMFTLPYLKAQDSRQNYEGLKIPEPGSSINVSRD
                     KIMSGISNVQKSETITNPDNPLNTFTVTTNADDGVGSFRDAITKANETSGADVI"
BASE COUNT          704 a          299 c          392 g          595 t
ORIGIN      
        1 cacataacag aaacacttct gcaaaattca tctttagcgt tgcgggcagg attgatttaa
       61 tagatggtat tgtacttgaa gatttctgcg aaatcgatgg ctctaatcaa attgaactta
      121 gtggtactgc aataatgacg acccaaaata atacaaagat caaagggctt agaatttcta
      181 cgatgtttgg agttgaactt ggaggaaaca acaatattgt tggtgatttc ccctattaca
      241 atgaatttgt taattgctat gttggtctga ctattaatgg tgacaacaac aaattattca
      301 ataattattt cggtattact caagatggca ctctaatgcc aaatcagttt ggaatgattg
      361 tgagtggaag taataatgaa attggtaaat ctgatatggc aaatggtaat gtagtatgcg
      421 gcaactcagt cgttggaata gaaatagctt tagggagtgg aaacaaaata caacataatt
      481 tgatcggaac aacctttgat gggagaacag acgtcgggaa tgggcaggga ataacaattg
      541 ggggatcaga taataatatt attggcggtg ataatttggt tgatggaaat gtgatttcgg
      601 gtaattcagt ctatggaata tttattagtg gtgcaccgcc tgatagttat tctgcagata
      661 acctgataaa gaataataat attggattaa atcctgataa aaccgcagct gtaccgaatg
      721 caagaggtat aaccattaca aatgcaacta tgagtgccca aattgtagat aatataattt
      781 ccggcaatac gaatgagggt atctacattt ttggatatga tgatgaaact tttaccaatg
      841 gtcatttaat tcatggtaac tcaattggca ccaatggagc ccaaggttct tttccgaatg
      901 atggtgatgg aattctaatt gcaggtaatg ttggaacagt aaaaatcgga caggatgaaa
      961 atgggaatta caccggtaat acaataattg gaaatggatc ggttggagtt aatattgtac
     1021 cacttctggg ttactcgcct gagcggatta ctgtaagaag aaatagtcta aatcagaatt
     1081 tattaacaaa tcttttcgta gatacttcag ccaataaccg gattagagaa ccttataatc
     1141 ttaattataa tgctggattg atttccggaa aacacgcatt gcctaatgca attatagaca
     1201 tttacagtgc taataaattt gaacttccgg catcagctta ttttagaatt ggaacaacaa
     1261 atacagatgc aaatggaaat ttttctttta gtacaaatct taatatagaa gctattgcag
     1321 ttaccgcaac cgattttgag ggtaatactt ctaactttgc aagattaaat attgtaacag
     1381 ctgttgaaga tgaaaaacaa attcctgctg aattttcttt agaacaaaac tacccaaatc
     1441 catttaaccc aagtacaaag ataagttggc agtctccagt gggcagtcat caaacattga
     1501 aaatttatga tgtgctaggt aatgaggttg ctacacttgt ggatgaatac cgcgaagcag
     1561 gaagttatga agttgaattt aacgcaagct tattaccaag cggcgtttac ttctacaaac
     1621 tgcaagaaga cggtttcgtt aaaacaaaaa aaatgactct aatcaaatga ccaaattatt
     1681 attcaaggag tttactgtga aaaaaataag tacgattgtc ttaattttaa tgtttacttt
     1741 accttatcta aaagcacaag attcaagaca aaattatgaa ggactgaaaa ttccagaacc
     1801 cggatcttca attaatgtct caagagataa aattatgagc gggatttcca atgttcaaaa
     1861 atcagaaaca attactaatc cggataatcc tttaaataca tttacagtaa caaccaatgc
     1921 tgatgatggt gttggttcat ttagagatgc aattacaaaa gctaatgaaa ccagcggggc
     1981 tgatgttatt
//
LOCUS       DASWBY010000783         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_446583, whole genome shotgun sequence.
ACCESSION   DASWBY010000783 DASWBY010000000
VERSION     DASWBY010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446583"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VF870_14230"
     CDS             complement(<1..320)
                     /locus_tag="VF870_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253400.1"
                     /translation="MKKQNIKKKEAAPTQKEEKLKTPISEVESRRKFLQKLWKGLGVV
                     ASVEVIAVIFGFLFSGKKEDQTLKPKQLFEAGNVESFKTNTVTTFRGGRFYLARLLDG
                     GFIA"
     gene            complement(321..1622)
                     /locus_tag="VF870_14235"
     CDS             complement(321..1622)
                     /locus_tag="VF870_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX9253401.1"
                     /translation="MQVSENTQKPKRFSGIILHLHPKFINERALSFNRTFGLGGMAAF
                     LIVLLFVTGILLRFYYEPFPQKAYNSIVILQNNVLFGQLIRNIHHWCGVFLVIISFLH
                     LLRVFFTGGFQSSRKLNWIIGLALFVFVIASNFTGYLLPWDQLAYWAITVGTSLLHYV
                     PFIGDSIRKFILAGDEVNANTLLIFFNFHTAVFPISILLLMAYHFWRVRKAGGIILPK
                     EVEEKKMIEVYPSLVYREFIVALALIAVILVFSVLFNAPLLAKANPDVSINPTKAPWY
                     FAGIQELLMHFHPFVAAFLIPFTVVFALAYLPYIKLKEEHTGKWFYSEKGKDAAKYSA
                     IAAALITIFLVVINEFIPDFETLIPWLNSFISNGIFPLIILLALLWYYYRFVLRKFKL
                     EIIEATQTMFVFVTTAFIILTVIGIFFRGIDMALTFPWNVL"
     gene            complement(1757..2626)
                     /locus_tag="VF870_14240"
     CDS             complement(1757..2626)
                     /locus_tag="VF870_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX9253402.1"
                     /translation="MFLTLRLSAQDLDPRAYARVPVDATVLIAGFNYTYGGVVLDPTL
                     PIKDLRAKVESPMLGIARTFSLFGLTSQAYAVLPYSWAQASGKVVGEDSSITRNGFGD
                     ARLRVSVLFLGAPAATVEEFAKSSPQTVMGASITVTAPTGQFFSDKLINLGTNRWSFK
                     PEIGLSYYINKHWLFDLYAGVWLFTENKSFYTGTSIRSQDPLISFQTHVSYSFNPLMW
                     AAIDFTYYTGGQSSVNNVYQDDRQNNSRIGATFNFPVSRVSTIKLAYSTGAVIRIGGD
                     FSTISIAWQTVFF"
     gene            complement(2724..>3147)
                     /locus_tag="VF870_14245"
     CDS             complement(2724..>3147)
                     /locus_tag="VF870_14245"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEX9253403.1"
                     /translation="SVLEPYLSKSIYSNQGQRVVSGQRLIQGAPDIFLGWGEQGGMQY
                     YVRQLRDMKGGVEFDPKKVKTENMDQYSSLCAWALALAHAKSGDAAMIAGYAGKSDEL
                     DEAMVNFAFAYADQTDKDYQALSAAAKSGRIKVASVSE"
BASE COUNT         1042 a          604 c          534 g          967 t
ORIGIN      
        1 aaagcaataa aaccgccgtc aagtaatctt gctaaataaa atcttccgcc tctgaaagtg
       61 gttacagtgt tagttttaaa cgattcaaca tttcctgcct caaacaattg ttttggtttt
      121 agagtttgat cttctttttt accagaaaac aaaaatccaa agattactgc aatcacttca
      181 accgaggcaa caacacctaa tcctttccac aacttttgaa gaaacttgcg tctgctttca
      241 acttctgaaa ttggtgtttt aagcttttct tctttctgcg taggtgcagc ttctttcttt
      301 ttgatatttt gttttttcat ttataaaaca ttccatggaa aagtaagtgc catatcaata
      361 ccacggaaaa atattccaat aacagtaagt attataaaag ctgtggttac aaaaacaaac
      421 atagtttgtg ttgcctcaat aatttctaat ttaaactttc ttaatacgaa tctataatag
      481 taccataaca acgcaagtaa aataatcaat ggaaaaattc cattgctgat aaatgaattc
      541 aaccatggta ttaaagtttc aaaatcaggt ataaattcat ttattacaac aagaaaaatt
      601 gtaattagcg cagccgctat agctgaatat tttgctgcgt cttttccttt ttcagaataa
      661 aaccatttgc ctgtgtgttc ttcttttagt tttatgtatg gcaaatatgc aagtgcaaaa
      721 acgaccgtaa atggaattaa aaatgctgca acaaatgggt gaaaatgcat tagtaattcc
      781 tgtattcctg caaagtacca aggtgctttt gttggattga tactgacatc tggatttgct
      841 ttggcgagca ggggagcatt aaataaaact gaaaagacaa gtatcactgc tatcaaagca
      901 agtgcaacaa taaattctct gtacaccaga cttggataaa cttctatcat tttcttttct
      961 tctacttctt tgggcaggat tatgccgcct gcttttctaa ctctccaaaa atgatatgcc
     1021 attaaaagca gaattgagat tggaaagacc gcagtatgaa aattgaagaa gatcaataat
     1081 gtatttgcat ttacttcatc acctgccagt ataaactttc ttatcgaatc accaataaat
     1141 ggtacataat ggagtaagct ggtacctaca gtaattgccc aatatgcaag ctgatcccat
     1201 ggaagcaaat agccggtaaa gttggaggca attacaaaga caaatagcgc tagaccaata
     1261 atccagttta attttcgtga ggattgaaat cctcctgtaa aaaaaactct gagcagatgg
     1321 agaaaagaaa tgataaccag aaatacaccg caccagtgat ggatgtttct gattagctgt
     1381 ccgaacaaaa cattattttg tagaattact attgaattgt atgctttttg aggaaacggt
     1441 tcgtagtaaa atcttaaaag tattcctgtt acgaaaagca gtacaattaa aaatgcagcc
     1501 atgcctccta aaccgaaagt acgattgaaa gaaagtgctc tttcattaat aaacttagga
     1561 tgaagatgga gaattattcc tgaaaagcgt ttaggttttt gagtattttc tgaaacttgc
     1621 attaatataa ttaaaacata aaaagaaaaa tttgctctga gaaattaacc aaaatagttt
     1681 acatttttta attagttaag tataaatctt tttctcttca taattcaatt atttaattaa
     1741 tccaattaaa ttctatttaa aaaaacaccg tctgccaggc tattgatata gtagagaagt
     1801 caccgccaat tcttataact gcaccggtac tgtatgctaa cttaatagta ctcactctgc
     1861 taaccggaaa gttgaatgtt gctccgatac gtgagttatt ttgtctgtca tcctggtaaa
     1921 cattgttaac tgatgattga ccaccagtat aataagtaaa gtctattgcc gcccacatta
     1981 agggattaaa actatagctt acatgcgttt ggaatgatat taatggatcc tggctgcgaa
     2041 ttgaagtacc agtgtaaaat gatttattct cggtaaataa ccagactcct gcgtatagat
     2101 caaataacca atgcttatta atataatagg ataatccgat ttcaggttta aatgaccagc
     2161 ggttagtacc aaggtttatt aatttatcgc tgaagaattg tcctgtaggt gccgtgacag
     2221 taatacttgc acccatcaca gtttgaggcg aagattttgc aaactcttca acggttgctg
     2281 ccggtgcgcc taaaaataaa acagaaactc ttaaacgggc gtcaccaaac ccattacgtg
     2341 tgatgcttga atcttcaccc actacttttc cggaagcctg tgcccaggag taggggagca
     2401 cagcatatgc ctgtgaagtt aaaccaaaca gactgaatgt ccgggcaatt ccgagcatcg
     2461 gcgattcaac cttcgctctt agatccttaa ttggtaacgt tggatccaaa acaacaccac
     2521 cgtatgtgta attgaaacca gcgattaaaa ctgttgcatc cacgggaact ctggcataag
     2581 ctcttggatc aagatcttgt gctgacaatc taagtgttaa aaacaaagat atactgatta
     2641 acagcacgga gtaatattta aatcgcactc ctcatgtcct ttttaagtat tgattctata
     2701 gcatggttga aaatatttta taattattca ctcacagatg ccactttaat tcttccactt
     2761 tttgcggcag cggaaagtgc ctgataatct ttatcagtct gatcggcata tgcaaatgca
     2821 aaattcacca tagcctcatc cagttcatca cttttacctg cataaccagc gatcatagcc
     2881 gcatcaccgg atttagcgtg ggccagagct aatgcccagg cacataatga actatactga
     2941 tccatatttt cagtttttac ttttttagga tcaaactcaa cgccgccctt catatctctt
     3001 aactgacgaa cataatactg cattccacct tgttcacccc aaccaagaaa aatatcaggt
     3061 gcgccctgta tcagccgctg tccggaaact actctttgcc cttgatttga ataaatagat
     3121 ttagaaagat aaggttcaag cacagag
//
LOCUS       DASWBY010000784         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_596464, whole genome shotgun sequence.
ACCESSION   DASWBY010000784 DASWBY010000000
VERSION     DASWBY010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596464"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /locus_tag="VF870_14250"
     CDS             complement(<1..1729)
                     /locus_tag="VF870_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX9253404.1"
                     /translation="MIFLKSLLYNLFIAAIVFSQATFSQVIFRDLPNYKINTSDQLFF
                     DITDTREIISLNGNWKVYPSDDEKKEKVTIRVPSIFEGKGEFVFEKSFPLTNSQLNNH
                     KISLNFFGLNYSADISVNKVIIYRHPGGEFPFTVSLPRDILRSDKDNVISVKLSYALD
                     SQNTIPLKQRFLFPQNFGGIIRDVYLHLTPNVSISDFTYKTDIDVKSNKAVIFLKSVI
                     DNKEFKKINDTLDAQTNFSLRTQIFYPDGKTSLSISNGKAGSGSGINDNSFILTRNKQ
                     IEITNSVTIASPTLWSPDNPASYIVRLELWRGDQLIDRYDQSIALFKLESSKENFSLN
                     GNEFNLYGVTYTPSFYQYGSLSSYDRMESDLEKIKQAGFNSVRFSKSLPNPYYLRLCE
                     QIGLFAFVDLPIANLPEGLAGSQNFVVRSKNYLSSLLKAYENFSAIGGIGFGSSYLFN
                     SDAHRALLSDLSSQVKKNRNVLTYASFIDFEYQQIEKLDLYGIEFLNQSPADKQNLVE
                     TIIKELGKGRLFIGEATYTVNIGQTDGYVNKFSYEAQAKFFDDIFSFYENNNLAGFFA
                     NTMYDLRGDY"
     gene            complement(1850..2605)
                     /gene="tpiA"
                     /locus_tag="VF870_14255"
     CDS             complement(1850..2605)
                     /gene="tpiA"
                     /locus_tag="VF870_14255"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9253405.1"
                     /translation="MRKTVIAGNWKMNNDLAQSEKLIVELKNLLQNEKINCDVIVCPP
                     FTSLSEATKFLKGSHIKLGAQNMHFEENGAFTGEVSAAMLKSVGCEYVILGHSERRHI
                     FGENDEVINKKIKKALSAGLKPIFCVGELLEERENGTTNDVVKRQVLKGLAGISADDM
                     KNIIVAYEPVWAIGTGKTASPAQAQEVHEFIRDLIEIDYSLEVANDLVIQYGGSVKPD
                     NAKELISQKDIDGALVGGACLKADSFLEIIKGA"
     gene            2845..>3024
                     /locus_tag="VF870_14260"
     CDS             2845..>3024
                     /locus_tag="VF870_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9253406.1"
                     /translation="MKSKISKQVLILGMVSLFTDIASEMLYPVTPIFLTTVLGSSMAL
                     VGVIEGVAEVTAGLLK"
BASE COUNT          995 a          527 c          481 g         1021 t
ORIGIN      
        1 gataatctcc gcgaagatca tacattgtat ttgcaaaaaa tcctgcaaga ttattgttct
       61 cgtaaaaact aaaaatatca tcaaaaaact ttgcttgcgc ttcgtaggaa aacttattta
      121 cataaccatc tgtctgcccg atgtttactg tataagtcgc ttctcctatg aataatctac
      181 ctttgccaag ttctttgata atggtttcaa ccaagttttg tttatcagca ggagattggt
      241 ttaaaaattc gattccatat aaatcaagtt tttctatttg ctgatattca aaatcaataa
      301 aagaagcgta ggttaaaaca tttctgtttt tctttacctg tgatgaaaga tcgctcaata
      361 aggcccgatg agcatcagaa ttaaacagat aagatgaacc aaatccgatt ccgccaatag
      421 cagaaaagtt ttcataggcc tttaacaaac tcgaaagata atttttactt ctaacaacaa
      481 aattttgtga gccggcaagt ccttccggta aatttgcaat tggcaaatca acaaaagcaa
      541 ataggccaat ctgttcacac aatcttaggt agtatggatt aggaagtgat tttgaaaatc
      601 taacggaatt aaatcctgcc tgcttaattt tttctaaatc gctttccatt ctatcataag
      661 aggaaaggct tccgtattga tagaatgacg gtgtataagt aacaccgtaa agattaaatt
      721 catttccatt taaagaaaaa ttctcctttg atgattccag cttgaaaagc gcaatactct
      781 gatcatatct gtcaatcaat tgatcaccgc gccaaagttc caatcgcaca atataagagg
      841 ctggattatc cggactccat aatgttggag atgcaattgt aacgctgttg gttatttcaa
      901 tttgtttatt ccgtgttaaa ataaaactgt tatcattaat acctgaacca gaacctgcct
      961 tgccgttaga tatgcttaaa cttgtctttc catcaggata aaatatttgt gttctaagag
     1021 aaaaatttgt ttgagcatca agtgtgtcat ttattttttt aaattcttta ttatcaataa
     1081 ctgatttcaa aaaaatgact gctttatttg attttacatc aatatctgtt ttataagtaa
     1141 aatcagaaat actaacattc ggagttaaat gaagatatac atcgcgtatt attccgccaa
     1201 agttctgggg aaataaaaat ctttgcttta atggaattgt gttttgtgaa tcaagcgcgt
     1261 aagaaagttt tacagaaatt acattgtctt tatcagatct aagaatatct cttggaagcg
     1321 aaacagtgaa tggaaattca ccgccaggat gacgataaat tattactttg tttacggaaa
     1381 tatctgcaga gtaatttaaa ccaaaaaagt ttaaggaaat tttatgattg tttaactggg
     1441 agtttgtaag cggaaagctt ttctcaaaaa caaactctcc ttttccttca aagattgatg
     1501 gaacccggat ggttaccttt tcttttttct catcatctga tggataaacc ttccagttac
     1561 cgttaagaga aattatctca cgggtatctg tgatatcaaa aaaaagctgg tctgaagtat
     1621 ttattttata gttaggaaga tctctgaata taacctgtga aaaagttgcc tgagagaaaa
     1681 ctattgcggc tataaacaga ttgtaaagta aacttttgag gaagatcatt ctttgctaaa
     1741 aaactcctaa aaaataagat acaaatataa cctcaaatcg aagggattgc aatgaaatga
     1801 aagcccgatt taagctggaa atcgggcttt tgaaaagaaa ataaaatatt tatgcgcctt
     1861 ttataatttc caggaaagaa tcagctttta agcaagcacc gccaactaat gcgccatcaa
     1921 tatctttttg tgaaataagc tctttagcat tgtcaggctt tacacttccg ccgtattgga
     1981 taactaaatc attcgcaact tctaaggagt aatctatttc aattaaatca cgaataaact
     2041 cgtgcacttc ctgtgcttga gcaggacttg cagtttttcc tgttccaatt gcccacactg
     2101 gttcgtatgc tacaataata tttttcatat catctgcgga aattccagct aatcctttta
     2161 gaacctgtct ttttactaca tcattagtgg ttccattttc tctttcctct aaaagctctc
     2221 caacacaaaa aattggtttt aatccggcag acaaagcttt tttgattttc ttatttatca
     2281 cttcatcatt ctcgccaaaa atgtgtctgc gttcagagtg accaagaatt acatattcac
     2341 atccaacaga ttttaacatt gctgcagaaa cttctcctgt aaaagctcca ttttcttcaa
     2401 agtgcatatt ttgtgcacca agttttatat ggctgccttt taaaaatttt gttgcttcag
     2461 ataaagaagt aaaaggcgga caaacaatta catcacagtt tattttttca ttttgtaaaa
     2521 gattttttaa ctcaacaatt agtttttctg attgagccaa atcattattc attttccagt
     2581 taccggcaat tacagtttta cgcatttttt cctcttaggt ttaataaaat ttctttgtaa
     2641 aattaacgaa gaataaaagg ttaaacaatt tatattctat acttttaagt gttgaaacaa
     2701 accaagaatg tcatttagaa ctagcctgcc tgtcggcaga caggtcttcg actgagaaat
     2761 ccgtttaatt ataaaattca gatctctcac ttcggttgaa aatgactatc tgatttcaat
     2821 ttattaaaat ttatttttag actaatgaaa tcaaaaatat ctaaacaagt tcttattctt
     2881 ggaatggtaa gtctttttac agacattgcc agcgagatgc tatatccagt aaccccgatt
     2941 ttcttaacaa ccgttttagg ctcatcaatg gcgcttgtgg gggttattga gggagtagca
     3001 gaagttactg ccggattgtt gaaa
//
LOCUS       DASWBY010000785         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_520816, whole genome shotgun sequence.
ACCESSION   DASWBY010000785 DASWBY010000000
VERSION     DASWBY010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520816"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /gene="ilvA"
                     /locus_tag="VF870_14265"
     CDS             <1..1112
                     /gene="ilvA"
                     /locus_tag="VF870_14265"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX9253407.1"
                     /translation="QIVRSYKIRGAYNKIFHLNKTGIKNGVVCASAGNHAQGVAYSCN
                     LLKIKGVIFMPTPTPKQKVNRVKMFGNGYVDIVLVGDTFDDAHKEALEYSVKNGCEFV
                     HPFDDPLVIEGQGTVGIEILEQTKDQIDFLFLPVGGGGLASGVSSVFKELSAETKLIG
                     VEPEGAPSMTKSFEQNKLVILESIDRFVDGAAVQKVGDITFEICKANLDKMITVNEGE
                     ICSNILKLYNEEAIVVEPAGALSVTALNQFKDEIKGKTVVCILSGNNNDITRTEEIRE
                     RSLLYEGLKHYFIISFSQRAGALREFVNFVLGPTDDISYFEYSKKNNRERGPAVVGLE
                     LQSKNDFDGLIQRMKTYGIVYEYLNERPELFHFII"
     gene            1206..>2033
                     /locus_tag="VF870_14270"
     CDS             1206..>2033
                     /locus_tag="VF870_14270"
                     /inference="COORDINATES: protein motif:HMM:NF037611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253408.1"
                     /translation="MKKTFFLFLAFQLLVISQTKKTPADYVNPFIGTKDMGHTFPGAT
                     IPFGMVQLSPDTDTIMYSYGKGYNPEVYRYCSGYQYDDSSIVGFSHTHFNGTGHSDLG
                     DFLIMPTIGSLQLNPGTRDNPDSGYRSRYSHQTEEAHPGYYKVKLDDNNILAELTATE
                     RVGFHKYTFPKSDSAHIILDLTSGIYNYDGKVVWSSIRVENDTLVTGYRQTNGWARTR
                     LLYFAMTFSKPIQSYGLVNKEELIYKGFWRKFDQNKNFPEQAGHKIVSCFDFKTESGE
                     "
BASE COUNT          695 a          290 c          392 g          656 t
ORIGIN      
        1 tacaaattgt aagatcttat aaaattagag gtgcttacaa taaaattttt catcttaaca
       61 aaaccggaat taaaaatggt gtagtttgtg cgagtgcagg taatcatgca caaggtgtcg
      121 catactcttg taacctgctt aaaataaaag gtgtaatatt tatgcccacg ccaactccaa
      181 aacaaaaagt aaatagagta aaaatgtttg gcaacggtta tgttgatata gttttggtag
      241 gagatacttt tgatgatgca cataaggaag cattagagta ttctgtaaaa aatggatgtg
      301 aatttgtaca tccatttgat gatccattag ttattgaggg gcaggggaca gttggaattg
      361 aaattcttga acaaactaaa gatcaaatag attttctttt tttaccagtc ggcggcggtg
      421 gattggcgtc cggagtttca tcagtattta aggaattaag tgccgaaaca aaattaattg
      481 gcgttgaacc ggagggtgct ccttcaatga ccaagtcgtt tgaacaaaat aaattagtaa
      541 tacttgaatc aattgataga tttgttgatg gagctgcggt acaaaaagtt ggggatatta
      601 cttttgaaat ttgcaaggca aatcttgata aaatgattac agtaaacgaa ggtgagattt
      661 gttccaatat tttaaaactt tataatgaag aagcaattgt tgtagagcct gccggcgcat
      721 tatccgtcac tgcactaaat caatttaagg atgaaataaa aggaaagact gttgtttgca
      781 ttttatcagg taataataat gatattacaa ggacagaaga gatacgcgaa cgttcattgt
      841 tgtatgaagg tttgaaacac tatttcataa ttagtttttc acagagggca ggggccttaa
      901 gggaatttgt aaattttgtt ttaggtccaa ctgacgatat ttcatatttc gaatattcaa
      961 aaaagaataa tcgcgaaagg ggccctgcag tggttggatt agaacttcaa tcaaaaaatg
     1021 attttgatgg tttaatacaa cgaatgaaaa catatggaat tgtttatgaa tacttgaatg
     1081 aaagaccgga gttatttcat tttataattt agccgattaa taaaatcagg aatattcttc
     1141 aataatctta gctgatattt tattgctcgt atatatcttt gctaagatta ttttaaaaga
     1201 atattatgaa aaaaacattt ttcctttttt tagcatttca attattagtt atttctcaaa
     1261 caaaaaaaac tcctgctgat tatgttaatc cgtttatcgg aacaaaggat atggggcata
     1321 catttcccgg tgccacaatt ccttttggga tggtgcagct aagtcctgat actgatacaa
     1381 taatgtacag ttatggaaaa ggttacaatc cggaagtata cagatattgt tcaggctacc
     1441 agtacgatga cagttcaata gttggatttt ctcacacaca ttttaatggt accgggcatt
     1501 ctgatcttgg tgatttttta attatgccta ccattggttc attacagcta aatcctggaa
     1561 caagagataa tcctgattca ggttatcgat ctcgttattc acatcaaaca gaagaagcgc
     1621 atccaggtta ttataaagta aaattagatg ataataatat tcttgctgaa ttaactgcaa
     1681 ctgaaagagt tggatttcat aaatacactt tccccaaatc tgattctgca catattattc
     1741 ttgatttaac ttcggggatt tataactatg atggaaaagt tgtttggtca tcaatcaggg
     1801 ttgaaaatga taccctggta acaggttaca gacaaacaaa cggctgggct agaactcgat
     1861 tactttattt cgcaatgact ttctcaaaac ctattcaatc ttatggattg gttaataaag
     1921 aagaacttat ctataaagga ttctggagaa agtttgatca gaataaaaat tttcctgagc
     1981 aagccggaca caaaattgta agttgctttg attttaaaac tgaaagcggt gaa
//
LOCUS       DASWBY010000786         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_260522, whole genome shotgun sequence.
ACCESSION   DASWBY010000786 DASWBY010000000
VERSION     DASWBY010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260522"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1304)
                     /locus_tag="VF870_14275"
     CDS             complement(192..1304)
                     /locus_tag="VF870_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX9253409.1"
                     /translation="MAEAGLFTLGVEEEFQIVDPVTRELKSHIQQILEDGKMILAENV
                     KAEMHQSVVEMGTDICRDVKDAREQVTRLRTDLAKLAMKNGLRIAASGTHPFSHWKDQ
                     KITEHPRYKVIVDDMQQVARANLIFGLHVHVGVEDREVAIHIMNAARYFLPHIFALST
                     NSPFWLGRNTGFKSYRSKVFDRFPRTGIPDFFNGVSEYDKFINLLIKTGCIDNAKKIW
                     WDIRVHPVFKTLEFRVCDIPMRVDETIALTAIMQALVAKLHKLIKQNLGFRLYRRALI
                     AENKWRAARYGINGKLIDFGKNEEVDFKILVGELLDFIDDVVDELGSREEVNYLHKIL
                     EMGTGADRQLAVWDQSHDTKNVVDYIIEETHYGLKL"
     gene            complement(1320..2276)
                     /locus_tag="VF870_14280"
     CDS             complement(1320..2276)
                     /locus_tag="VF870_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253410.1"
                     /translation="MKKIGILFGQENTFPQAFVDRVNSKKENGIIAELAHINKIIQGE
                     PIGYDVLIDRISQDVPFYRAMLKNAAISGTGVINNPFWWSADDKFFNNALATKIGVAV
                     PKTVILPSNQHPTDTNERSFRNLAYPLDWEGIFEYIGFPAYFKPFAGGGWKNVYKLNN
                     MDEFFKAYNETGQLVMMLQEEIIFTEYFRCYCLDRQDVHIMQYDPKQPHEFRYVRNPK
                     PLEKKLLDDVHNGVLELNKALGYDFNTVEFAIRDGVPYAIDFCNPAPDADVNSVGAEN
                     FEWIVEAAANMAIRKAKAHKPGQNNLTWGSFVTSFANNKPLK"
BASE COUNT          717 a          483 c          379 g          796 t
ORIGIN      
        1 catacttcaa atttacgttc tgattatttt cacttacatc agagacaatc tctttaatac
       61 aacgcatgcc ttcgttacgt tcgttgttgt aaagatctat tgttgcaact ttaatttgtg
      121 gtttattcat aattagtctt cttttgaacc ccatccaaca ttccctttca gaaaggggag
      181 tatttggaag gttacagttt taatccgtaa tgtgtttctt caataatata atcaaccaca
      241 tttttagtat cgtgtgattg atcccaaaca gcaagttgtc tatcagcacc agtgcccatt
      301 tctaaaattt tatgaaggta attaacttct tctctagaac ccaattcatc cacaacgtca
      361 tcaataaaat ctaataattc gccaacaaga attttaaaat cgacttcttc attcttccca
      421 aaatcaatta atttcccatt tattccgtat cgtgcggctc gccatttatt ctcagcaatt
      481 aatgctctgc ggtataatct gaatccaaga ttttgcttaa ttagtttatg aagttttgca
      541 acaagtgctt gcataatagc agttagtgca attgtttcgt caacacgcat aggaatatcg
      601 caaactctaa attccaatgt tttaaataca ggatgaacac gaatatccca ccagattttt
      661 tttgcgttat cgatgcagcc tgttttaata agcaagttta taaacttatc atattcagaa
      721 actccgttaa aaaaatccgg aatgccggtt cttgggaatc tatcaaatac tttgcttctg
      781 taagatttaa agccagtatt tctaccaagc caaaacggag aatttgttga tagagcaaaa
      841 atatgcggca gaaagtatct tgctgcattc ataatgtgga ttgccacttc acgatcttca
      901 acaccaacgt gaacatgtaa tccaaaaatc aaattagcac gggcaacttg ctgcatatca
      961 tcaactataa ctttgtaacg cggatgctcc gttatttttt gatctttcca atgagaaaat
     1021 ggatgagtac cggaagcagc tatccttaat ccatttttca tagcaagttt tgcaagatca
     1081 gttctaagtc ttgtaacttg ttcacgtgcg tcttttacat cccgacaaat atcagttccc
     1141 atttcaacaa ccgattgatg catctctgct tttacatttt cagcaagaat catcttgcca
     1201 tcttcaagaa tttgctggat gtgagatttt aattcccttg ttactggatc aacaatctga
     1261 aattcttctt caactccgag tgtaaacaaa cctgcttcag ccataatgac tcttctttct
     1321 tatttcaaag gtttattgtt tgcaaacgat gtaacaaatg atccccaggt aagattattc
     1381 tgtccgggtt tgtgggcctt tgcttttctg atagccatat tagctgcagc ctcaacgatc
     1441 cattcaaaat tttctgctcc cactgaattt acatcagcat caggtgccgg attacagaaa
     1501 tcaatagcgt atggcacacc atcacggatg gcaaattcaa ctgtattaaa atcataaccc
     1561 agtgctttgt taagttcaag aactccgttg tgaacgtcat ccaataattt tttctcaagt
     1621 ggttttggat ttcttacata acgaaattca tgcggctgtt ttggatcgta ctgcataatg
     1681 tgcacatcct gtctatctaa acaataacag cggaaatatt ctgtaaaaat aatttcttcc
     1741 tgaagcatca ttacaagttg tccggtttca ttatatgctt taaagaattc atccatatta
     1801 ttcagtttgt atacattttt ccatccaccg ccggcaaaag gtttaaagta agcaggaaaa
     1861 ccgatgtact caaaaattcc ttcccagtcg agtggataag caagattacg aaatgaacgc
     1921 tcatttgtat cagttggatg ttgatttgat ggcagtataa ctgttttagg aaccgcaact
     1981 ccgatttttg tagcaagcgc gttattaaaa aatttatcgt ctgcgctcca ccaaaatgga
     2041 ttgttaataa cccctgtgcc ggaaattgca gcattcttta acattgcgcg ataaaaagga
     2101 acatcctggg aaattcggtc aatcaaaaca tcataaccaa tcggttcacc ctgaataatt
     2161 ttattgatgt gggcaagttc agcgatgatt ccgttttctt tttttgaatt tacacgatca
     2221 acaaaagctt gaggaaaagt gttttcctgt ccgaaaagaa ttcctatttt tttcattgtt
     2281 aactctcaaa ttattttaat aaatccgaat cctccttgct cctcccgtct cagaaaggga
     2341 agtaaggtga gattaaactt taattttgct taaat
//
LOCUS       DASWBY010000787         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_184090, whole genome shotgun sequence.
ACCESSION   DASWBY010000787 DASWBY010000000
VERSION     DASWBY010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184090"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VF870_14285"
     CDS             <1..249
                     /locus_tag="VF870_14285"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253411.1"
                     /translation="LTLLFDLEPSIGLNRIAKHDDRENNRLYVESLAFHESVREGYKE
                     VVKRYPQRIQVIDASKSQEEVTKNVLKVLRKSLDSRMK"
     gene            305..634
                     /locus_tag="VF870_14290"
     CDS             305..634
                     /locus_tag="VF870_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX9253412.1"
                     /translation="MKLIVAVVQDQDSSRLSSALTKGDFRATKLASTGGFLRSGNTTF
                     LIGTEDDLVSNALDLIRDNCRSREQMVAPVSPMGGNADSYIPYPVEVEVGGATVFVLP
                     IEQFHHF"
     gene            737..1741
                     /gene="holB"
                     /locus_tag="VF870_14295"
     CDS             737..1741
                     /gene="holB"
                     /locus_tag="VF870_14295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX9253413.1"
                     /translation="MNANLKDVRKLQPVVMKQLSTVFQKNRVAHAYLFEGAKGTGKVA
                     VMRSFVKLLLCEQPIENVSCETCRGCRRFESGNHPNFIQIEPDGQDIKKDQMLQLIFE
                     MNKTALEAGRKIYVLHRADRLNASAANTLLKFLEEPEGLVTAILLTEKAHAILPTIRS
                     RSQIISFPSIPLDERVKVMLDEGLTASMAATVSMVTNDLEESLRLAKDEQFAQVRKTV
                     LKLVEAIETNVHEAMLTVHEDWLPLLKEKEETEQALDLLLFAYRDLVAVKANSNATYT
                     YPDFLPFYQQRALHITYEQLSNKIEAILKAKQQLHRNMNRTLLMEQLMLNLQGGYSFV
                     "
     gene            1734..>1818
                     /locus_tag="VF870_14300"
     CDS             1734..>1818
                     /locus_tag="VF870_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation protein"
                     /protein_id="HEX9253414.1"
                     /translation="MYNVVGVRFKKAGKIYYFDPIDFPLEKD"
BASE COUNT          589 a          288 c          397 g          544 t
ORIGIN      
        1 ttaacattat tgttcgatct ggagccgtca atcggattaa atagaattgc aaagcacgat
       61 gatagagaga ataatcgact ctacgttgaa agtttggcat ttcatgaatc cgttagagaa
      121 ggatataaag aagtcgttaa acgttatcct cagcgtattc aagtgattga tgcatccaaa
      181 tcgcaagaag aagtgacaaa aaatgtactg aaggttctaa gaaaatctct tgactcacgc
      241 atgaaatgat ctgtcttttc atgttataat attaggaaga acttccggaa aaggggttga
      301 gctgatgaaa cttatcgtgg ctgttgtcca agatcaagat agtagtcgtt tatctagtgc
      361 actgacaaaa ggtgattttc gtgcaactaa gttagcgagt actggtgggt ttttacgttc
      421 aggaaataca acgtttttaa tcggtacaga ggatgattta gtatctaatg cacttgattt
      481 aattcgagat aattgccgtt ctcgtgagca aatggtagct cctgtatctc ctatgggagg
      541 caatgcggat tcatacattc cttatccagt tgaagttgaa gttggtggcg cgacagtatt
      601 cgtgcttcca atcgaacagt tccatcattt ttaaacgatt tattcacaat taattttatg
      661 tagccataca cgaaaatccc gtgttttccg gatatccgga gcacggtttt ttttgaaaga
      721 aagggtgagc aacgaaatga atgcaaacct aaaggacgta agaaaattac aacctgttgt
      781 tatgaagcaa ctttcaacgg tttttcaaaa aaaccgagtt gctcacgcat atctttttga
      841 aggagcaaaa ggcactggta aagttgcagt gatgcgatct tttgtgaaat tattgttatg
      901 tgaacaaccg attgaaaatg tttcatgtga aacatgtcgc ggctgtagga gatttgagtc
      961 aggcaatcat ccgaatttta ttcagattga gccagacggt caagatatta agaaagatca
     1021 aatgttacaa ctgatttttg aaatgaataa aacggctcta gaagcgggtc gtaaaatata
     1081 tgtattacac agggcagacc gattaaatgc ttcggctgcc aataccttac tgaaattttt
     1141 agaagaacct gaaggattgg ttacggctat attattgacg gaaaaagcac atgcgatttt
     1201 accaacgatt cgatcacgta gtcaaattat ttcttttcca tcgattcctc ttgatgaacg
     1261 agttaaagta atgttagatg agggtcttac agcatcgatg gcggcaaccg tttctatggt
     1321 aactaatgat ttggaagagt cattacgatt ggcaaaagat gagcaatttg cacaggttcg
     1381 aaaaacagtg ttaaaattgg tagaggcaat tgaaacgaat gtacatgaag cgatgctaac
     1441 agtacatgag gattggttac ctttgttaaa agaaaaagaa gaaacagaac aagcattgga
     1501 tttactttta ttcgcatacc gcgatttagt ggcagttaag gctaatagta atgcaacata
     1561 tacgtatccg gatttcttgc cattttatca acaacgtgct cttcacatta cgtatgagca
     1621 gttgtcaaat aaaattgagg ccatattaaa ggcgaagcaa caattgcatc gcaatatgaa
     1681 tcggacgctg ttaatggagc agttaatgct gaatttgcag gggggatatt catttgtata
     1741 atgtggtagg tgtccgcttt aaaaaagcgg gtaaaatata ttattttgat ccaattgatt
     1801 ttcctttgga aaaagatc
//
LOCUS       DASWBY010000788         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_596852, whole genome shotgun sequence.
ACCESSION   DASWBY010000788 DASWBY010000000
VERSION     DASWBY010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596852"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VF870_14305"
     CDS             <1..888
                     /locus_tag="VF870_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253415.1"
                     /translation="KTFKTSVAEPNWWSYRALWALTEGYDFIIKCEGNRSSRVKQGIS
                     KIIESIKKNIPEEKNEVTIEGIKLPDWLPFQFASDQAALLIITLSNYYKITGDKEILD
                     YINSLAHGIMMMQIKDSECEFNGAFLSWQNSWHGWGNSQAYALLKAYQIIENEELKKA
                     ALFELDNFYKRLFQIGFLSSFSVRKTDCKFEIIESNKFSQIAYDIRPMVYALLEAFKV
                     SSDTSYAIQAGQIAKWFIGKNPPSEVMYNPHTGIFYDGIENDNLINKNSGAESTIEGL
                     LTLLNISQNKSALISFQSE"
     gene            918..>2379
                     /locus_tag="VF870_14310"
     CDS             918..>2379
                     /locus_tag="VF870_14310"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX9253416.1"
                     /translation="MLKKIKLNDWNFSLLKNYKSSNLLIDKRLIPSRKFPATVPGTIH
                     TDLLDNKLIDDPFYSDNELKMDWIPECDWQYQTNFDFKGDLKNNIDLVFEGLDTISEI
                     YLNGLKLGGTDNMFVQYRYNVKNILKAKGNELKILIKSPIRYAADEENKYGRLPVALN
                     STRVYIRKAQYSFGWDWGPSFPTSGIWRNVYLEEWSVAKIENVTFRTISINKKTAEVE
                     VNVDVNISEKKEISLEISLVNEDIFYDQVISLTSSLKNKISFRIKEPKLWWPNGEGEQ
                     NLYLLKVKLLKDNIVLDEIQKNVGIRTVGLVLKAKDKSTFKFRVNNKDIYSKGVNWIP
                     ADSFLPRVSKNKYYELLSLAKQANMNIVRVWGGGIYEDDEFYNLCDKLGLLVWQDFMF
                     ACGSYPENDNFVASVSEEVTQNVFRLQHHACIAIWCGNNENEWIWYQEQKSSYKKMPG
                     YNIYHKIIPDILKVIDPSRRFWPSSPFGDDYDPNSFE"
BASE COUNT          870 a          334 c          434 g          741 t
ORIGIN      
        1 aaaactttta agacaagtgt tgcagaaccc aattggtggt cataccgcgc actctgggca
       61 ctgacagaag gatatgattt cattataaag tgtgaaggta atagaagcag cagggttaaa
      121 caaggcatat ctaaaattat tgaaagcatt aaaaaaaata ttcctgaaga aaaaaatgaa
      181 gtgacaattg aaggaatcaa actccctgat tggcttccat ttcaatttgc atcggatcag
      241 gcagcattgt taataataac cctttctaat tattacaaaa taactggaga caaagaaatt
      301 ctcgattata taaattcact tgcacacgga attatgatga tgcagataaa agattccgaa
      361 tgtgaattta acggagcatt tttaagctgg cagaatagct ggcatgggtg gggaaattca
      421 caggcctatg cgttactaaa agcctatcaa atcattgaaa atgaagagtt aaaaaaagcc
      481 gcattatttg aactggataa tttttataaa agattatttc agatcggatt tctaagttca
      541 ttcagtgtta gaaaaacgga ttgtaagttt gaaataattg aatcaaataa atttagtcag
      601 atagcatacg atattagacc tatggtttac gcattacttg aagcattcaa agtatcgtca
      661 gatactagtt acgctattca agcaggtcaa atcgcaaaat ggtttattgg gaaaaacccg
      721 ccaagtgaag ttatgtataa tccgcatact ggaatatttt atgacggaat tgaaaatgat
      781 aatctgataa ataaaaactc cggtgctgaa tctacaattg aaggattatt aaccctgctt
      841 aatatttcac aaaataaaag tgcattaata tcttttcaat ctgagtgaat gattataatt
      901 aaaatgaaat atgtgctatg cttaaaaaaa ttaaattaaa tgactggaat ttttctttat
      961 taaaaaatta taaatcatct aatttattaa ttgataaaag attaattccc tccagaaaat
     1021 tcccggcaac tgttcccggt acaattcata ctgatttatt agacaataaa ttaattgatg
     1081 atccatttta ttcagataat gagcttaaaa tggactggat accggagtgt gactggcagt
     1141 atcaaaccaa ttttgatttt aaaggggatt taaagaataa tattgatctt gtttttgaag
     1201 gtttagatac catcagtgaa atctatttga atggtttaaa actcggtggc actgataata
     1261 tgtttgttca atacagatat aatgttaaaa acattctgaa agctaaagga aatgaattaa
     1321 agattttaat taaatcaccg ataagatacg ccgctgatga agaaaacaaa tatggcagac
     1381 ttcctgtcgc cttaaattca acccgggttt atataagaaa agcacaatat tcattcggtt
     1441 gggactgggg accatctttt cccacctcag gtatttggcg taatgtatat ctggaagaat
     1501 ggtctgttgc aaaaattgaa aatgtaactt ttcgtacaat cagcattaat aaaaaaactg
     1561 cagaagttga agtaaatgtt gatgtaaata tttctgaaaa aaaggaaata tcattagaaa
     1621 tatctctcgt aaatgaggat attttttatg atcaagtaat ttcacttact tcatctttaa
     1681 aaaataaaat tagttttaga attaaagagc ctaagctatg gtggccaaac ggtgaaggtg
     1741 agcagaactt atacttatta aaagttaaac ttcttaaaga caatattgtt cttgatgaaa
     1801 tccaaaaaaa tgtcggtatc cggaccgttg gattagtctt aaaagcaaaa gataaatcta
     1861 cgtttaagtt tcgggtaaac aataaagata tttatagtaa aggtgtaaac tggattcccg
     1921 ctgattcgtt tctcccaaga gtaagcaaga ataaatatta tgagctgctt tcgttagcaa
     1981 agcaggctaa tatgaatatt gtaagagtct ggggcggtgg gatttatgaa gatgatgaat
     2041 tctataatct atgcgataaa ctcgggttat tagtttggca ggattttatg tttgcctgcg
     2101 gctcatatcc ggagaatgat aattttgtcg caagtgtaag tgaggaagta actcaaaatg
     2161 tttttcgtct acaacatcac gcttgtattg caatttggtg cggtaataat gaaaatgaat
     2221 ggatatggta tcaggaacaa aagtcttctt acaaaaaaat gccgggatat aatatttatc
     2281 ataaaattat accagatatt ttaaaagtaa ttgacccttc acgccgattc tggccttcct
     2341 caccatttgg agatgattat gatccgaatt catttgaaa
//
LOCUS       DASWBY010000789         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_33431, whole genome shotgun sequence.
ACCESSION   DASWBY010000789 DASWBY010000000
VERSION     DASWBY010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33431"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VF870_14315"
     CDS             complement(<1..602)
                     /locus_tag="VF870_14315"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX9253417.1"
                     /translation="MIINRIAILGLGLIGGSFAKAIKHSDASIHISAYDRPDILNKAL
                     NEKVIDEELKSINDSLNSDLIILALPIEQSLKVFKELSPRLKPNQIISDLCSVKGIFA
                     DEWKSVSSAGRYFGAHPMTGKEKSGYENSDLLLYENSVFIICTKDETDETLNSYINFI
                     KLIGARIVLLDAHLHDRITSHVSHLPQLLSVLLMNQANNK"
     gene            complement(599..>1716)
                     /gene="aroF"
                     /locus_tag="VF870_14320"
     CDS             complement(599..>1716)
                     /gene="aroF"
                     /locus_tag="VF870_14320"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX9253418.1"
                     /translation="KPGSLVDALSVFRDYQINMTKLESRPIIGNPWEEMFYLDFQGNI
                     QDQKVKNLLDEVGKHTRYLKILGCYPAKYVEKTKVERIQIKTDEIKENVVLPEATPQK
                     KSASKVSYKLASREYKSDDTIISVRDVKIGGGSFIIMAGPCSVESLNQILDCAKEVKD
                     NFAHILRGGCFKPRTSPYAFQGLGFEALDYLKSAGDDYDLPIITEVLSVDQVERVAEK
                     SDILQIGARNMQNFSLLSEVGKSFRPVMLKRGLMASIEELLNAAEYILARGNRQVILC
                     ERGIRTFETATRNTLDLSAIPVLKELTHLPIIVDPSHAVGKRDKVLPLAKAAKAVGAD
                     GIMVEIHPDPSKALSDGEQSMRFDQFRLMTQELEKMN"
BASE COUNT          516 a          337 c          278 g          585 t
ORIGIN      
        1 attttgttat ttgcctgatt cataagtaat actgaaagca actgcggtag atgcgaaacg
       61 tgggaagtta ttctatcgtg caaatgagca tccagtaaaa caattcttgc accaatcagt
      121 ttaatgaaat ttatgtacga atttaatgtt tcatcagtct catcttttgt gcagataata
      181 aaaacggaat tctcataaag taataaatca gaattctcgt aaccgctttt ctcttttcct
      241 gtcattggat gggcgccaaa atatctgccg gcactggaaa cagatttcca ttcatctgca
      301 aatattcctt ttacactaca aagatcagaa atgatttggt ttggttttaa tcttggagaa
      361 agttctttga agacttttaa actttgttca attggtaatg ctaatattat taaatcagaa
      421 ttcagagaat catttatact ttttaattct tcatcaataa ctttttcatt caaggctttg
      481 tttagaatat caggtctatc atacgcggag atatgaatac ttgcatccga atgtttaatg
      541 gcttttgcaa aagatccgcc aattaaccct aaacctaaaa tggcaattct gttaatgatc
      601 aattcatttt ctcaagttct tgtgtcatca atctaaattg atcaaatctc attgactgtt
      661 ctccatcact tagtgcttta gacgggtcag ggtggatctc aaccataatt ccatcggcac
      721 caacagcctt ggcagcctta gccaatggaa gaactttatc acgtttacca actgcatgtg
      781 atggatcaac aattatcggc aaatgagtca attcttttaa aaccggaatt gcgcttaaat
      841 ctagagtgtt acgagtagcg gtttcaaaag ttctaattcc gcgttcacat aaaattactt
      901 gtctgtttcc acgtgcaaga atatactctg ctgcattcag cagttcctct atagatgcca
      961 tcaatccgcg ttttaacata acagggcgaa atgatttacc aacttctgaa agcagagaaa
     1021 aattttgcat atttcgtgca cctatttgaa gaatgtcaga tttttcagct actctttcca
     1081 cttgatcaac tgataaaact tcggtaataa ttggaagatc atagtcatca cctgctgatt
     1141 taagatagtc aagtgcttcg aatcccagtc cctgaaatgc atagggtgaa gttcttggtt
     1201 taaagcatcc tccccgtaaa atgtgtgcaa aattatcctt tacttctttt gcacaatcca
     1261 gtatttgatt caaggattca actgagcagg gacctgccat aattataaaa ctaccaccgc
     1321 caatttttac atccctaact gaaattattg tgtcatcact tttgtattcc cggcttgcaa
     1381 gtttatagga aactttggaa gcagatttct tctgaggagt agcttcaggt agtactacgt
     1441 tttcttttat ttcatctgtt tttatttgga ttctttcaac cttagttttt tcaacatact
     1501 ttgcaggata gcacccaaga atttttaagt aacgtgtatg cttcccaact tcatccaaca
     1561 aatttttaac tttttgatcc tgaatgtttc cttgaaaatc gagataaaac atttcttccc
     1621 acggatttcc aattatcggc cgtgattcaa gctttgtcat atttatttga taatcacgga
     1681 atactgacaa agcatcaaca agagaacccg gcttat
//
LOCUS       DASWBY010000790         2920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_107784, whole genome shotgun sequence.
ACCESSION   DASWBY010000790 DASWBY010000000
VERSION     DASWBY010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107784"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VF870_14325"
     CDS             <1..306
                     /locus_tag="VF870_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9253419.1"
                     /translation="DVGEVVLITHEKCIGCKACVEACPYDARFIHPDGYADKCTFCIH
                     RVEKGQKPACVEVCPTYCMHFGDLDDPNSEVSKLLQSRKWHVNLQDAGTQPNIFYLV"
     gene            317..1273
                     /gene="nrfD"
                     /locus_tag="VF870_14330"
     CDS             317..1273
                     /gene="nrfD"
                     /locus_tag="VF870_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX9253420.1"
                     /translation="MNELLELTTTRNNPHVDPSMAMWEWQIPVYLFLGGMVAGMMIIS
                     GYFLFSGRYKESNCACFSIPFISVILLSLGMFALFLDLSHKLYVWRLYTTFQIKSPMS
                     WGAWILILVYPALLANILMRPTPWMLRIYPKLSEWAGKINQHPFLIKNIGITNMILGM
                     MLGAYTGVLLSSMGSRPLWNTSLLWVLFLVSGLSAAAAFVHLFAKNKSESELLAKADN
                     GFLVIELFVFVMIFLGFLSSTRPHMEAANLLLTGPYAPVFWVFVIGLGIVIPLIIQLL
                     AVNHKIRHTAIAPIMVILGGLILRFVIVSAGQYSHWFNAHFH"
     gene            1288..1869
                     /locus_tag="VF870_14335"
     CDS             1288..1869
                     /locus_tag="VF870_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEX9253421.1"
                     /translation="MNSLTKKITDAFEKDEGHEHIHINAKPYSNPYLVGIGLGLVLLA
                     AFVIMGRGLGASGALSTMVAVTVNTIAPEHAAGNSFYSEYIGDGNTNPLMDWLIFQTL
                     GVLVGGFISGTVSHRVIKGVEKGPRISNKMRYVYAFIGGGLLGYGAKLARGCMSGQAL
                     SGGAVLNLGSWVFMMMVFVGGYAAAYFMRRQWT"
     gene            1860..2471
                     /locus_tag="VF870_14340"
     CDS             1860..2471
                     /locus_tag="VF870_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEX9253422.1"
                     /translation="MDMNAPFFKFGLFNSEVSLIIAFIIGIAFGIALEKGGFGRATIL
                     ASQFYFTNMRVLKVMFTAIVTAMLGLYFLSVIGFVDLSLVYLTQTNLLPMAVGGLIAG
                     LGFIVGGYCPGTSMVAVATGKIDAVIYVVGMIFGIFAFGEMFPGIENFFNSGYMGTVT
                     LPAFFNLSYGLVVFLVVLMAVGAFAAAEWGEKKMAAKTGKEIL"
     gene            2468..>2920
                     /locus_tag="VF870_14345"
     CDS             2468..>2920
                     /locus_tag="VF870_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9253423.1"
                     /translation="MKKFFSNLSLNKKLALIAITLGVIALVAGDPYDRTTIKVNEKAI
                     ALSTVDNSDKVSVTELADWIIKDKSDFELIDLRSEEKFNEYSIPNAQSIPLPQISSSE
                     LLRNQKIVLFSDDDIEASQAWFILKSKNYKGVYILTGGLNAWKDKVLFP"
BASE COUNT          846 a          460 c          625 g          989 t
ORIGIN      
        1 gatgttggcg aagtcgttct aataactcac gaaaaatgta ttggttgtaa agcctgcgtt
       61 gaggcttgtc cttatgatgc acgttttatt catcctgatg gttatgctga taaatgtact
      121 ttctgtatac acagagttga aaaaggacaa aagccggctt gcgttgaagt ttgtcctacg
      181 tactgtatgc attttggtga tcttgatgat ccaaacagcg aagtcagcaa gctgcttcag
      241 tcgcgcaaat ggcatgtaaa tttacaagat gctggtacac aaccaaatat attttattta
      301 gtgtaaggga ttaaagatga atgaattact tgaattaact acaacaagaa ataatcctca
      361 tgtagatcca tctatggcaa tgtgggaatg gcagataccg gtttaccttt tcttaggtgg
      421 aatggtggct ggaatgatga tcatttcagg ttatttttta ttcagcggca gatacaaaga
      481 aagtaactgt gcctgctttt cgataccatt tataagtgtt atccttttaa gtcttggaat
      541 gtttgcttta tttctggatt tatcacacaa actttatgta tggaggttat acacaacatt
      601 ccaaatcaaa tcaccaatgt cttggggtgc atggatattg atccttgttt atccggcact
      661 gttagcaaac atcttaatgc gcccaacacc ctggatgttg agaatctatc ccaaactttc
      721 tgagtgggcc ggaaaaataa atcagcatcc atttttgata aagaacattg gaatcacgaa
      781 tatgattctt gggatgatgc tgggtgctta cacaggtgtg ctgctcagct ctatgggttc
      841 acgtccgtta tggaatactt ctttgctctg ggttttattt ttagtttcag gattatctgc
      901 tgcggctgca tttgttcacc tatttgcaaa aaataaatct gaaagtgaat tgcttgcaaa
      961 agctgataat ggatttcttg taatagaact ttttgttttt gtaatgatct ttcttggatt
     1021 tttatcatct acaagaccgc atatggaagc agcaaatctt ttattaacag gcccatatgc
     1081 tcctgttttc tgggtattcg taatcggttt aggaattgta attccactga taattcaatt
     1141 gcttgcagta aatcataaaa ttcgtcacac tgctattgca ccaataatgg taattcttgg
     1201 tggattaata ctaagattcg taattgtatc ggctggtcaa tacagccatt ggtttaacgc
     1261 acattttcat taatggagat taattaaatg aattctttaa ctaaaaagat cactgatgcc
     1321 ttcgaaaaag acgaagggca tgagcacata cacataaatg cgaaacctta ttcaaatcct
     1381 tacttagttg gaattggatt gggattagta ttgcttgcag cctttgtgat aatggggaga
     1441 gggttgggag cttccggagc actttcaacg atggttgcag taaccgttaa cacaattgca
     1501 ccagaacacg ctgctggtaa tagtttttat tctgaatata tcggtgatgg aaatacaaat
     1561 ccactaatgg attggttgat ttttcaaact ttaggggtct tagttggtgg ttttatttct
     1621 ggtacagttt ctcatcgtgt aataaaagga gttgagaaag gtccgcgcat ctcaaataag
     1681 atgagatatg tatacgcatt tattggcgga ggattgctcg gatacggcgc caaacttgca
     1741 cgtgggtgca tgagtggaca agcactttcc ggcggtgctg ttttaaatct tggcagctgg
     1801 gtatttatga tgatggtttt tgtgggcggc tatgccgctg cttattttat gaggaggcaa
     1861 tggacatgaa cgcaccgttt ttcaaatttg gtttatttaa ttcagaggta agtctaataa
     1921 ttgcatttat aatcggaatt gcttttggaa tcgcattgga aaaaggagga tttggaagag
     1981 caacaattct tgcttctcaa ttttacttta caaatatgcg agtattaaaa gttatgttta
     2041 cagcaattgt tacagcaatg ctgggattat attttttatc cgtaatagga tttgttgatc
     2101 tttctcttgt ttatttgacg caaacaaatt tattacctat ggctgttggt ggattgatcg
     2161 ctgggttagg atttattgtg ggcggttact gtccgggtac ttcaatggtt gcagtagcta
     2221 caggcaagat agatgctgtt atttatgtgg tcggaatgat ttttgggata tttgcatttg
     2281 gagagatgtt tcccggaatt gaaaactttt ttaactcagg ttatatgggc actgtaactc
     2341 ttccggcgtt ttttaatctt tcttatggtt tagttgtttt tcttgtcgta cttatggcag
     2401 tgggggcatt tgcagcagca gaatggggtg aaaagaaaat ggctgcaaag actgggaagg
     2461 aaatcttatg aaaaagtttt tctcaaatct tagcttgaat aaaaaacttg cacttatagc
     2521 aattacactt ggtgttatag ccctggttgc aggtgatcct tatgacagaa caacaattaa
     2581 agtaaatgaa aaggctattg cattatctac agttgataat tcggataaag tatcagtaac
     2641 ggaattagcc gattggatta ttaaagataa atcagatttt gagctaattg atttaagaag
     2701 cgaagaaaaa tttaatgagt actcaattcc caatgctcaa tctattccac ttccacaaat
     2761 ttcatcatct gaattattaa gaaatcaaaa aatagtttta ttctcagatg atgatattga
     2821 agcctctcaa gcttggttta ttcttaaatc aaaaaactac aaaggggttt atatcctcac
     2881 tggtggattg aatgcgtgga aagataaagt acttttccca
//
LOCUS       DASWBY010000791         3331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_747624, whole genome shotgun sequence.
ACCESSION   DASWBY010000791 DASWBY010000000
VERSION     DASWBY010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747624"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VF870_14350"
     CDS             <1..279
                     /locus_tag="VF870_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560151.1"
                     /note="ATP-dependent carboxylate-amine ligase; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX9253424.1"
                     /translation="NKWRAARYGINGKLIDFGKNEEVDFKILVGELLDFIDDVVDELG
                     SREEVNYLHKILEMGTGADRQLAVWDQSHDTKNVVDYIIEETHYGLKL"
     gene            341..1174
                     /locus_tag="VF870_14355"
     CDS             341..1174
                     /locus_tag="VF870_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253425.1"
                     /translation="MNKPQIKVATIDLYNNERNEGMRCIKEIVSDVSENNQNVNLKYD
                     VFETRYKGDIPDLNYDIFISSGGPGSPFEDEDKKWDKDYFTLLDKIWNHNETSEQKKY
                     IFFICHSFQMMARYFQFGEVNKRFINSFGVMPFQKTDEGKNDPILKDLTDPFYAADIR
                     HWQVVNPDQKKIAEIGAKILSWEIPEAENKDNPAITSIRISNEIVGTQFHPEADPESM
                     LYHFKQDERKKFIIDRYNEDRYWEMIGLLEHPDKIKLTRKTVIPAFLNNAINDLISVT
                     V"
     gene            1177..2358
                     /locus_tag="VF870_14360"
     CDS             1177..2358
                     /locus_tag="VF870_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253426.1"
                     /translation="MIPEIRKKFNNDFSEKVYQEFIDDLNSVLKYPPDFRVCETPLFL
                     SHELTSELVKACDDLIVQIQQEDFLKKSEQAIPAHLKVPNDIEHPPFLQLDFAITKNG
                     EKFWPKLIELQAFPSLYGYQAYLNDVIRKHFDIPVGFHSYFSGLNRTAYRSLLTKIIV
                     GNHDPENVILLEIEPEKQKTRIDFAATEELTGITTVCLSKIKKRGKKLFYVKDAKDIQ
                     VKRIYNRVIFDELERKNAKFDFDFKVEIDAEWAGHPNWFFKISKFSLPMLKGNYVPEC
                     YFLNEIDKYPENLKDFVLKPLFSFAGLGVDVEVTKEKLNAVENRSNYILQQKVEYAPL
                     IDTPDGFSKVEIRMMFLWENKDEKPILVNNLIRTSKGKMMGVDFNKNKTWIGSSIAYH
                     P"
     gene            2581..>3331
                     /locus_tag="VF870_14365"
     CDS             2581..>3331
                     /locus_tag="VF870_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253427.1"
                     /translation="MITYYFHITKDSRIKYKIEENLFSIKGNLLISDFRTARLLSEKI
                     NAVRRDEGKFELQVTAGQINALGLLHEIFHLLIRKYEEDNKKGVFKAGIDYLKNNLTE
                     EELDNTLLKFVEEFPPLPVYQNKLNAIEYLNAKTENKDNREILLEELIILNLENINPA
                     THQLKELYTDEKLLKETKYKEIIEHTELFFEREPKIGGFNLISFLRKPIIQSPYNIEE
                     QLDFIRANWSKFIDETFLSKILSGKDLIHEDY"
BASE COUNT         1220 a          509 c          569 g         1033 t
ORIGIN      
        1 aataaatggc gagccgcacg atacggaatc aatgggaaat taattgattt tggaaagaat
       61 gaagaagttg attttaaaat tcttgttggc gaattattag actttattga tgacgttgtt
      121 gatgaattgg ggtctagaga agaagtgaat taccttcata aaattcttga aatgggcact
      181 ggtgctgata gacagcttgc tgtttgggat caatcacacg acacaaaaaa tgtggttgat
      241 tatattattg aagaaacaca ttacggatta aaactgtaac cttccaaata ctcccctttc
      301 tgaaagggaa tgttggatgg ggttcaaaag aaaactaatt atgaataaac cacaaattaa
      361 agttgcaaca atagatcttt acaacaacga acgtaacgaa ggcatgcgtt gtattaaaga
      421 gattgtctct gatgtaagtg aaaataatca gaacgtaaat ttgaagtatg atgtttttga
      481 aactcggtat aaaggcgata tacctgattt aaattatgat atttttattt catcaggtgg
      541 acccggaagt ccttttgaag atgaagataa aaaatgggat aaagattact tcacattgct
      601 ggataaaatc tggaaccaca acgagacatc agaacagaaa aaatatattt tcttcatttg
      661 ccattccttt cagatgatgg caagatattt tcaatttggt gaagtaaata aacgatttat
      721 taattcgttt ggggttatgc cgtttcaaaa aactgatgaa gggaaaaatg atccaatctt
      781 aaaagattta accgatcctt tttatgcagc ggatataaga cattggcagg ttgttaatcc
      841 tgatcaaaaa aagattgctg agattggtgc aaaaattctt tcgtgggaaa ttcctgaagc
      901 agaaaataag gacaatcccg caattacatc aataagaatt tcaaacgaaa tagtgggtac
      961 acaatttcat ccggaagctg atcctgaaag tatgctctat cactttaagc aagatgaaag
     1021 gaaaaagttt ataatcgatc gctacaatga ggacaggtat tgggaaatga ttggattgct
     1081 tgaacatcct gataaaataa aacttactag aaaaactgtt attcctgctt ttttaaataa
     1141 tgctattaac gatttaattt ctgttacggt ttaaacatga ttcctgaaat cagaaaaaag
     1201 tttaataatg atttttcaga aaaagtttat caggaattta ttgacgattt aaattcagtt
     1261 ttaaaatatc ctcccgattt tcgcgtttgt gagactcccc ttttcttatc acatgaactg
     1321 acaagcgagc ttgtaaaagc ttgtgatgat ttaattgttc aaattcagca ggaagatttt
     1381 ttgaaaaaat ctgaacaagc tatccctgct cacttaaaag ttccaaatga tattgagcac
     1441 cctccctttt tgcagcttga ttttgctatt acaaaaaacg gagaaaagtt ttggccaaaa
     1501 ttaattgagc ttcaggcctt cccttctcta tatggttacc aggcttatct taatgacgta
     1561 ataagaaaac attttgatat ccctgtcgga tttcatagct acttcagcgg acttaacaga
     1621 actgcgtacc gctcattact tacaaaaata attgtcggca accacgatcc tgaaaacgta
     1681 atacttcttg aaatcgagcc ggagaaacaa aaaacaagaa ttgattttgc agcaacagaa
     1741 gagttaacag gaattactac cgtttgttta tcaaaaatta aaaagcgtgg taaaaaacta
     1801 ttctatgtta aagatgctaa agacattcaa gtaaagcgaa tatacaaccg tgtaatattt
     1861 gatgaacttg aacgaaagaa tgcaaaattt gatttcgatt ttaaagttga gattgatgct
     1921 gaatgggcag gtcatccaaa ctggtttttt aagattagta aattttcatt acctatgtta
     1981 aaaggtaatt atgttcccga atgctatttc ttaaatgaga tagataagta tcctgaaaat
     2041 cttaaggact ttgttttaaa accccttttt tcctttgcag gacttggtgt tgatgttgaa
     2101 gtaacaaaag aaaaactgaa cgctgtagaa aacagaagca attacattct gcagcaaaaa
     2161 gttgagtacg cacctttgat tgacacacca gatggttttt ctaaagttga aatccgaatg
     2221 atgtttttat gggaaaataa ggatgaaaaa ccaatactgg taaacaacct tatccgcaca
     2281 agcaaaggta aaatgatggg agtagatttt aacaaaaaca aaacctggat tggttctagc
     2341 atagcatacc atccttaatt ttattttgta tttatttatc cagatcgatc aggagatcga
     2401 tcgctgctat tcattcacag attttaacct tcgttcaaaa gagcaattaa tttttatttt
     2461 tatatagaat caagcgaaga tctattctaa aaaatttcaa tcattttata ccaataattt
     2521 attatatttc accccgaatc attgatattc tgttcaaaat ttaacctcaa gtaaaacatt
     2581 atgattactt attatttcca cattacaaaa gactcaagaa taaaatacaa gattgaagaa
     2641 aacctttttt caatcaaagg caatctttta atttcagatt ttcgcacagc aagattgtta
     2701 tctgaaaaaa ttaatgctgt tagaagagac gaaggcaaat ttgaacttca ggtaactgcg
     2761 ggacaaatta atgctctagg tttacttcac gaaatttttc atcttttaat tcgcaaatat
     2821 gaagaagata ataaaaaagg tgtctttaaa gccggaattg actatttaaa gaataatcta
     2881 actgaagaag aacttgataa tacattattg aaatttgtag aagagtttcc gccacttccg
     2941 gtttatcaaa acaaactaaa tgcgattgaa tacttaaacg ctaaaactga gaataaagac
     3001 aaccgtgaaa tacttcttga agaacttatc atattaaatc ttgaaaatat taatcctgct
     3061 actcatcaat taaaagagct atacacagat gaaaaacttt taaaagaaac taagtacaaa
     3121 gagataattg agcacacaga gttatttttt gagcgtgaac caaagattgg cggatttaac
     3181 cttatatcat tccttcgtaa acctataata caaagtccat ataacattga agagcagctt
     3241 gatttcattc gtgcgaactg gagcaaattc atcgatgaga catttttaag taaaattctt
     3301 tccggcaagg atttaataca cgaagattat a
//
LOCUS       DASWBY010000792         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_628163, whole genome shotgun sequence.
ACCESSION   DASWBY010000792 DASWBY010000000
VERSION     DASWBY010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628163"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VF870_14370"
     CDS             complement(<1..778)
                     /locus_tag="VF870_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253428.1"
                     /translation="MKELRKSFFVLCIILYSFIFSMNTTAQIRNKPLSEYLGAFAVPL
                     EIGNVWNFRNANFQPEFRVELFDTVFINGHKYYSLKRQDIEGYIYKNEYLRLREDGYF
                     VCFDSLYSFFPQQDYIFYKLNASKGDSWTQQDMTNKNYYHNIIDSIEISSFWGTYLLV
                     KIVEVTDSVLTTYWEYWSDEFGMIQQQTEEFGAGGWYYLWGCYINGQQYGDTVLVSVD
                     EANRKYLTDFKLHQNYPNPFNSNTIINYEVAKSAFVSIIVY"
     gene            complement(1147..>2743)
                     /locus_tag="VF870_14375"
     CDS             complement(1147..>2743)
                     /locus_tag="VF870_14375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253429.1"
                     /translation="AHHVIVQESNSLKYYKMDANGNSDNAITIESSSVVSPSICGDNN
                     RLYVVYRKSSENYIRTKFSSDGGSTWSYLSSNPQNSNANSMESVFSNNKLHVTFDVSN
                     VAYYSYYNTENPGWSSLQTVSYSENGTTPRITALYTSTQDRVMFLYKKTSTESRWREW
                     NVTNNSWATNPQTAFTVSSSYSSDPKGIAVDANYIYSYYEFTVYNPYGIYSQLQQRLR
                     SNNSLVTTSVAQSELSVPKIFSTTTTDNISHTAFYYNGVLEGGYEIGIMRNKHTGSGI
                     SADPAYPYEFGSNQPTMINISSASNDVFVVWKDGLSNYLKLVYDDQNPLAPANYSVSA
                     YQSGTNYYPRLTWSLNNEPDVRGNSTDAYKIERRTRPLNGTWSSWSELANLSGTTSSY
                     IDYTINNASGGDREAEYRITAIDIGDNTSSTQSVIIEYGQNILDKMKINGMVSNYALD
                     QNYPNPFNPSTKISYSIKEDGLVTLKVYDVLGKEIATLVNENKATGNYEVDFNASELP
                     SGVYIYTLQVNGFTSSKKMLLMK"
BASE COUNT          883 a          482 c          426 g          952 t
ORIGIN      
        1 cataaacgat tattgaaaca aaagctgatt ttgcaacttc ataattgata attgtatttg
       61 aattaaacgg gttaggataa ttttgatgta acttaaaatc agtaagatat tttcggtttg
      121 cttcgtcaac tgaaactaaa acagtatcac catattgctg cccatttata taacaacccc
      181 aaagataata ccagccacca gcgccaaatt cttctgtttg ttgctgaatc atcccgaact
      241 catctgacca atattcccaa taagtagtga gaacactatc agtaacttca acaattttta
      301 caagtaagta tgtaccccaa aaagatgata tttctataga atcaataata ttatgataat
      361 aattcttatt agtcatatcc tgctgtgtcc aagaatcacc ctttgaggca tttaatttat
      421 aaaaaatata atcttgttgt ggaaagaaag aatataaact atcaaagcaa acaaaataac
      481 catcttctct aagtcttaaa tattcgtttt tatatatata accttcaata tcttggcgct
      541 ttaatgaata atatttatga ccattaataa acaccgtatc gaatagttcc acgcggaact
      601 caggttggaa attagcattt ctaaaattcc aaacattacc aatttctaaa gggactgcaa
      661 aagctcctaa gtattccgag agtggtttgt ttcgtatttg tgcggttgta ttcattgaga
      721 atataaaaga atagagaatt atacaaagaa caaaaaatga ttttcgtaat tctttcataa
      781 agttacctct ttacttttta agtaaattat ttttatgttt atacgaatta aatgagaaaa
      841 ataaatgatg tgaaaaactc tgtgcgggga gggtatctta aaatggtttt ttagtaacat
      901 ctctccaccc caataaaatt tgtgcagcaa gagaaagtta aatatcgtgc cagaaaatct
      961 tcaacagaaa atattttcgg atacgaaacc accaatgcct cattaattaa ctactacaaa
     1021 cgaaaagaaa aaaaggcaca aagtcaaatt aaattttaat ctaaatgctt gccaatcacc
     1081 aaataacaaa ggcacgatct ctcgtgcctt taacttctaa ccgctagctt attacttctt
     1141 accctattac ttcattaaca acattttttt gcttgatgta aagccattta cctgtaaggt
     1201 gtagatgtaa acgccacttg gtaattctga agcattaaaa tcaacttcat aatttcctgt
     1261 agctttattc tcatttacta gcgttgcaat ttcttttcct aaaacatcat aaacttttag
     1321 tgttactaat ccatcttctt ttattgaata agaaattttt gtgcttggat tgaatgggtt
     1381 aggataattc tgatctagag cgtaattact taccattccg tttatcttca ttttatcaag
     1441 tatgttttgg ccatactcta ttattaccga ttgtgttgat gatgtattgt ctccgatatc
     1501 tatcgcggta attctatact ctgcttctct atcgccgcct gatgcattgt tgattgtgta
     1561 atcaatatat gatgatgttg tgcctgataa gtttgcaagt tctgaccagc ttgaccaggt
     1621 tccatttaat ggtcttgttc ttctttctat tttataggca tccgttgaat tacctcttac
     1681 atcaggttca ttgtttaaag accaggttaa tctgggataa taatttgtac cagactgata
     1741 agctgatact gagtaatttg ccggtgcaag aggattctga tcatcatata caagttttaa
     1801 gtaattgctt agaccatctt tccatacaac aaatacatca ttggaagcag aagatatgtt
     1861 tatcatcgtt ggttgatttg aaccaaattc atatggatac gccggatcgg ctgatattcc
     1921 actgcctgtg tgtttatttc tcattatccc tatttcataa ccgccctcta aaacaccatt
     1981 gtaataaaat gctgtatgag aaatattatc agttgttgtg gttgaaaata tttttggcac
     2041 tgataattct gactgagcaa cactcgtggt taccaatgaa ttattagatc ttaatctttg
     2101 ctgaagttgg gaatatattc catatggatt ataaactgta aattcataat aactgtagat
     2161 gtaatttgca tccactgcaa ttcctttcgg atcgcttgaa taacttgatg ataccgtaaa
     2221 tgctgtttgc ggatttgttg cccaactgtt atttgttaca ttccactcac gccagcggct
     2281 ttcggttgaa gttttcttgt ataaaaacat aactctatcc tgtgtgcttg tgtacaaagc
     2341 tgtaattctt ggcgttgtac cattttcaga gtaggaaacg gtttgcaatg aactccaacc
     2401 tgggttttca gtgttgtaat aagaataata agctacatta gaaacatcaa aagtaacgtg
     2461 taacttattg ttagagaaaa cggattccat tgaattggca ttactatttt gaggattgga
     2521 tgacaagtag ctccaggtac tacccccatc actgctaaat tttgttctga tataattttc
     2581 actgcttttt ctgtaaacaa catataacct gttattatcg ccgcaaatac ttggagaaac
     2641 aactgaagaa ctttcaatcg taattgcatt atctgaattg ccattagcat ccatcttata
     2701 atattttaga gaattacttt cctgaacaat aacgtgatgt gca
//
LOCUS       DASWBY010000793         5516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_33544, whole genome shotgun sequence.
ACCESSION   DASWBY010000793 DASWBY010000000
VERSION     DASWBY010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33544"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            547..1032
                     /locus_tag="VF870_14380"
     CDS             547..1032
                     /locus_tag="VF870_14380"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9253430.1"
                     /translation="MKWADAEVWKKVLGFSLAENDQKIKKLLYHLHQVQYAFYFLWNN
                     LPLEIPKQEEFVNLKSIAKWGFDYQYKLNEFLSTPAAKGKEKLIQVPWSVFIERKTGK
                     KVIPATLEETMLQVASHSTYHRGQINTRFRELGGEPAIVDLIVWVWSGKLKVNWMDVV
                     K"
     gene            1029..1970
                     /locus_tag="VF870_14385"
     CDS             1029..1970
                     /locus_tag="VF870_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9253431.1"
                     /translation="MKNISALIFVSIFFIFSVKAQTVKVPSYYQIDQKLSSDLQSIVH
                     ELHLDQNFDVGEDGIEQISLAVIDLTGNQPKLGGVNYDNFIYPASVYKMYVASEILNQ
                     VSQGKYSLYETYVAKEPNVVDRSKQIDNDPRPLLRDNDTVNVNYLLDLMITRSDNSAA
                     NCLIDIAQRKNIDSLLHSYGWYGSEVTRKYLKRKYEDPGYENIRGTETCALHAADFMY
                     KIYSNTLVNEWVSLQMKTLLGRQLDVSKLSKGLPQSAMFYHKTGWFSYWTNDVGIVDD
                     GEVKYIIACFIPLEEELALPKFKALSEKVYDLIKSRL"
     gene            complement(2062..2982)
                     /locus_tag="VF870_14390"
     CDS             complement(2062..2982)
                     /locus_tag="VF870_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9253432.1"
                     /translation="MQTILGSTGVIGTELAKSLLQYTYKIRLVSRNPKKVNPTDELFS
                     ADLLNADQVLKAVEGSDVVYLTAGLQYKISVWQTQWPIIMKNVINACKTYKTKLVFFD
                     NVYAYGLVKGWMKEDTLVNPSSKKGEVRAQIAQMIMSKVEKGQLDAIIARAADFYGPH
                     TPLSFATVTVFENLKKGKKAQWFLDASKKHSMTYTPDAGKATAILGNTNSAYNQIWHL
                     PTDKNVLTGKEFIELAAQAFGVEPKYTVLKKWMIQMVGIFVPVVEESIEMLYQNEYDY
                     LFDSTKFEQAFNFTPTSYKDGIAETVKSMR"
     gene            complement(3141..3647)
                     /locus_tag="VF870_14395"
     CDS             complement(3141..3647)
                     /locus_tag="VF870_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253433.1"
                     /translation="MKLYYLIFSLLIFFTACSGTDESNPVNTEISATGFVRGTVNGSN
                     WYSNKITTSKSNNTRIVKATQELPNNSKFSSSVMEIRISVSQAGIFGIGENEPGYTYY
                     IKAYYKLVSRNGIEDENYKAYFDNISYMTINRISDKDLNAVFNFNARTDDSTKTISLS
                     SGSIQIDY"
     gene            complement(3787..4608)
                     /locus_tag="VF870_14400"
     CDS             complement(3787..4608)
                     /locus_tag="VF870_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253434.1"
                     /translation="MKKIFFILPFIVLILGFTAGHIIDENLQALLKQFKIEEAAAKSD
                     VLYAVSGPSFYIPNVKVLRDLAVGDRISLIESIGKNVKEYVGSKEFVEKYNQLREDRK
                     PTPPEAPKYSAQLKEEQKQSLSNSIAEMEKSKSQMAKDQQATFDDIIKGLKDQLKEID
                     NPDNPVFTPEMDVYIKQGYDMQMSDYKNKVTEWEAEYPVNNPKPMIQKWINAFLEKSA
                     DINFDAKTTKNKDGMLKFVDQQYEYKDSQWKLYYRAGKESVTAARSFAQNWLKEL"
     gene            4766..5332
                     /locus_tag="VF870_14405"
     CDS             4766..5332
                     /locus_tag="VF870_14405"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX9253435.1"
                     /translation="MKTKIRCPWPANDKLMIEYHDKEWGVPLHSDRKLFEFLLLEGFQ
                     AGLSWRTILYKRQNFRKAFDNFDFNKVAKYDKRKINSLMKDAGIIRNQLKINSAVSNA
                     KVFIAVRKEFGTFDKYIWSFVNGKPIKNNFKSLKELPARTELSDLISKDLKKRGFNFV
                     GSTIIYAHMQATGMVNDHLKSCFRYNEL"
BASE COUNT         1781 a          915 c          937 g         1883 t
ORIGIN      
        1 ttaatggaaa aagaggcaag tgctctgtgg gcaaaaatgt cttcatttat tacttccatt
       61 gataataaat atggcaatcc cagaccagat cttggatatg ttaaaaagta attaatattt
      121 tttattcatc agccgataca aataatattt gtatcggctt ttttgtttaa taatatttca
      181 ttcgataatt aaaaaccttt ttcaaaagaa atatgctgac cttgaaatgg gtcctttatt
      241 aaaaaaacat cactatgagc aaagaatata aacctatagc ttgcggactt tatgatgaac
      301 ttgaactcag cgctttaagg aaataacccg ttaagcttgc ttttataaat tctaaaaatc
      361 aaactgaaat aattgagtgt atcattgttg atttgttttc aaaagataaa acagaatttc
      421 ttaaagcgga cgatggaaaa gtcattcgcc ttgatgatgt ggtggaactg gataatataa
      481 tttttaataa aaactgctga aagaattaat atgaattcgg gatcaataga aaccttaata
      541 aaccatatga agtgggcaga tgcagaagtc tggaaaaaag ttttggggtt ttcgcttgca
      601 gaaaatgatc aaaaaataaa aaaactttta tatcaccttc accaggttca atatgccttt
      661 tattttctct ggaacaattt gccacttgaa attccaaagc aggaggagtt tgttaatctt
      721 aaatcaattg ctaaatgggg atttgattat cagtataagt taaatgagtt tctttcaact
      781 ccggctgcaa agggaaaaga aaaattaatt caagttccat ggtcggtctt tattgaaagg
      841 aaaacaggga agaaagtaat tccggcaacc ttagaagaaa ctatgcttca agttgcttct
      901 cacagcactt atcatcgtgg acaaattaat acaagatttc gtgagcttgg tggtgagcct
      961 gcaattgtag atttaattgt ctgggtttgg tccggtaaat taaaagttaa ttggatggat
     1021 gtggttaaat gaaaaatata tcagcgctga tatttgtatc catatttttt attttttctg
     1081 taaaagcaca gactgttaaa gtgccctctt attaccagat agatcaaaaa ctttcttcag
     1141 atttacaaag cattgttcat gaacttcatc ttgatcaaaa ctttgatgtt ggtgaagatg
     1201 gaattgaaca aatctcttta gctgtaattg atttaaccgg caatcagcca aagcttggcg
     1261 gagttaatta tgataatttt atttatccgg cctccgttta caaaatgtac gttgcttcag
     1321 aaattctgaa tcaggtttca caagggaaat attctttgta tgaaacttat gttgcaaaag
     1381 aaccaaatgt tgtcgataga tcaaaacaga ttgataacga tccgcggcct ttgttacggg
     1441 ataatgatac tgtaaatgtt aattatttgc tggatttgat gattacgaga agcgataact
     1501 ccgctgcaaa ttgcttaatt gatattgcac agagaaaaaa cattgatagt ctgttacact
     1561 cttacggctg gtacggaagt gaagtaacaa gaaaatattt gaaaaggaaa tatgaagatc
     1621 ccggttatga aaacattaga ggaaccgaaa cctgtgcatt gcatgctgca gattttatgt
     1681 ataagattta ctctaacact ttagttaatg aatgggttag cctgcaaatg aaaactttgc
     1741 tgggaagaca attggatgtt tcaaaacttt caaaaggttt acctcaaagc gcaatgttct
     1801 atcacaagac tggctggttt tcttactgga caaatgatgt agggattgta gatgatggcg
     1861 aagtgaaata tataattgcc tgtttcattc ctcttgaaga agaattggca ttgccgaagt
     1921 ttaaggcgct atcagaaaag gtttatgatt tgataaaaag cagattatga tactgtttct
     1981 tggcgttctt agcgtttttg tctttgcggg ctttgcgtga actgctttaa cttccggtgt
     2041 acgcaaagat tattcagcga ctcacctcat tgatttaacc gtttcagcaa ttccatcttt
     2101 ataacttgtt ggcgtaaaat taaatgcttg ctcaaacttc gtactgtcga ataaataatc
     2161 atattcattc tggtataaca tttctatact ttcctccaca acaggaacaa atattccaac
     2221 catttgaatc atccattttt ttagcaccgt gtatttgggt tctacaccaa aggcttgtgc
     2281 tgccagttca ataaattctt ttcctgttaa aacattttta tctgtcggta aatgccaaat
     2341 ttgattgtaa gcagaatttg tattgcctaa aattgctgta gctttgccag catccggagt
     2401 gtatgtcata gaatgctttt tgcttgcatc cagaaaccat tgagcttttt ttcccttttt
     2461 taaattttca aacactgtta ctgtggcaaa acttaatggt gtgtggggac cataaaaatc
     2521 agcagctctg gctattatag catcaagctg tcccttctca accttactca ttatcatctg
     2581 tgcaatttgt gctcgtactt cgcccttttt acttgaggga tttacaagcg tatcttcttt
     2641 catccagcct tttactaaac catatgcata aacattatca aaaaaaacta atttagtttt
     2701 ataagtttta catgcgttta taacattttt catgataatc ggccattggg tttgccagac
     2761 actaattttg tactgcagcc cggcagttaa ataaacaaca tccgagcctt caacggcttt
     2821 caaaacttga tctgcgttta acagatctgc agaaaataat tcatcggttg gatttacctt
     2881 tttaggattt cggcttacaa gtctgatttt ataggtgtat tgtaatagtg attttgcaag
     2941 ctctgtaccg attactcctg ttgagcctaa tatcgtttgc atttaaacct ctgggtacga
     3001 ataataaatg taaatttatg ctaaatatcg gttaaggctt tgactttatt tttagcagaa
     3061 atcacattta atctaaaaac aacaaaggaa tagttaatta gtgcagtttt gtgatttgga
     3121 tcgcaaaaat ataattaagg ttagtaatct atctgaattg atccgctgct taatgagatt
     3181 gtttttgttg aatcatcggt acgagcatta aaattaaaaa cagcgttaag gtccttatca
     3241 gatattctat taattgtcat ataacttata ttatcaaaat aagctttata attttcatcc
     3301 tctattccgt tgcgagaaac aagtttataa taagctttaa tataataagt ataccctggt
     3361 tcattctcgc caataccaaa tatgcccgct tggcttacac ttattcttat ctccataact
     3421 gaagaagaaa atttggaatt gttaggaagt tcttgggtgg ctttaacaat acgggtgtta
     3481 ttgcttttgc tggttgttat tttatttgaa taccagtttg atccgttaac agttcctcta
     3541 acaaacccgg ttgcgcttat ttctgtattt acaggattcg attcatcagt accagagcat
     3601 gcagtaaaaa atatcagcaa agaaaaaatt agataatata gtttcatata ataatagtaa
     3661 gttttataat gactgtgaat ctaatctatt tggctgtaaa caaaaacgga ataatatttc
     3721 caaaagcaag atccctctct gaaaattagc agagagggat tgggtcggat gatcttaaaa
     3781 aaaatatcaa agttccttta accaattttg tgcaaaagat cttgccgcag taacggattc
     3841 tttgcctgcg cggtaataca atttccactg gctgtcttta tactcatact gctgatcaac
     3901 aaattttaac atgccgtctt tgttctttgt ggtttttgca tcaaaattaa tatctgcgga
     3961 tttttctagg aaagcattta tccacttctg aatcattggc tttggattat taaccggata
     4021 ttcagcctcc cactcggtaa ctttattttt ataatcactc atctgcatat catatccctg
     4081 ttttatatac acatccatct ccggtgtaaa tacaggatta tccggattat caatttcctt
     4141 taactggtcc tttaacccct ttattatatc atcaaacgta gcctgctgat cttttgccat
     4201 ttgtgatttt gatttttcca tctctgctat gctattactt aaactttgtt tttgctcctc
     4261 ttttaattga gcagaatact taggagcttc gggaggagtt ggttttctat cctctcttag
     4321 ctgattgtat ttttccacaa actcttttga tccaacatat tcttttacgt ttttaccaat
     4381 agattcaatc aatgaaattc tatctcccac tgcaagatcc cgaagaactt taacatttgg
     4441 aatataaaaa gaaggtccgc tgacagcata taaaacatcc gactttgcgg cagcttcttc
     4501 aattttaaat tgtttaagca acgcttgcag attctcatca ataatatggc cagccgtaaa
     4561 gccaagaatc aggactataa acggaagaat gaaaaatatt tttttcataa gacacactcc
     4621 tggttaaatt aatatttatg ctcactccaa aaattgatat acaattattt atctacaaat
     4681 tatatttaaa gtattttttg gatgattagg ctccggaatg ttatttttaa aatagattat
     4741 tattcaaaat aattatagta aaaagatgaa aacaaaaatc cgctgcccct ggcccgccaa
     4801 tgataaacta atgattgagt accacgacaa agaatggggt gttcctttac acagcgatag
     4861 aaaattattt gaatttcttt tactggaagg atttcaggca ggattaagct ggcgaaccat
     4921 actttacaaa agacaaaact tccgtaaagc atttgataac tttgatttta acaaagttgc
     4981 aaagtatgat aaacgaaaaa tcaactccct aatgaaagac gcaggaatta ttcgtaatca
     5041 attaaagata aattctgcgg ttagtaatgc aaaggttttt attgctgtta gaaaagaatt
     5101 tggaactttt gataaataca tctggagttt tgtaaatggt aaaccgatta aaaataattt
     5161 taaatcttta aaagagctcc cggctcgtac agaactttca gatttgataa gcaaagattt
     5221 gaagaaacga ggattcaact ttgtcggttc aacaattatt tatgcgcata tgcaggcaac
     5281 tggaatggtt aatgatcatc ttaaaagttg ttttagatat aacgagttat aataaaaata
     5341 attctttcct ccgattgact taaagtgctt ttatgctcat attgaaataa ctttgaaaat
     5401 tgagttttgc acaacaacta tttcaagaat aaacataaga ggcccggtat gaaaattttt
     5461 actattttct tttcaatttt gcttttctca accttaatgg ctcagagtca gccgca
//
LOCUS       DASWBY010000794         5447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_668078, whole genome shotgun sequence.
ACCESSION   DASWBY010000794 DASWBY010000000
VERSION     DASWBY010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668078"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VF870_14410"
     CDS             complement(<1..500)
                     /locus_tag="VF870_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9253436.1"
                     /translation="MLDKNITEIKESFYSDIKSVSSIQDVEEIRIKYFSRNGLVSKLF
                     EDLKDVVKEEKPILGKKLNLLRNEVSTYFEEVKERISSTQSSKENEIDLTLPGKEYII
                     GSKHILTQTLDEIKSIFKGLGFSVANGPELESDYYNFESLNFPADHPARDMQDTFFVS
                     KDFLLRT"
     gene            complement(569..916)
                     /gene="rplT"
                     /locus_tag="VF870_14415"
     CDS             complement(569..916)
                     /gene="rplT"
                     /locus_tag="VF870_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX9253437.1"
                     /translation="MPRSKNKVASHRRRRNQLKRAKGYWGNQGNVLTAAKNAIDKGLG
                     HAYRDRKLKKRVYRSLWIVRINAAARTNGTTYSRLIDALNKKGVDINRKVLASLAAEN
                     PTAFSEVVKFSIN"
     gene            complement(930..1124)
                     /gene="rpmI"
                     /locus_tag="VF870_14420"
     CDS             complement(930..1124)
                     /gene="rpmI"
                     /locus_tag="VF870_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX9253438.1"
                     /translation="MPKMKSNRGASKSFKKTASGSFKRKKAYKSHILTSKTTKRKRSL
                     RKGTLVSDAEQKRVRIMVQK"
     gene            complement(1126..1671)
                     /gene="infC"
                     /locus_tag="VF870_14425"
     CDS             complement(1126..1671)
                     /gene="infC"
                     /locus_tag="VF870_14425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX9253439.1"
                     /translation="MAGKQTVRINEEIRAAKVRVIDVDGSQLGVYTVRDAIRLSEERG
                     QDLVEIAPQAEPPVCKIIDFGKFKYEQQKRDKIQKKNQVVSILKEIRMHPNTDVHDFD
                     FKVKHAINFLEEGNKVKVSVMFKGREMAYTDQGEELLKRFIEKLEEVAKVESPIKLEG
                     RNMATILVPQSKKVKKTNKQG"
     gene            complement(1693..3630)
                     /gene="thrS"
                     /locus_tag="VF870_14430"
     CDS             complement(1693..3630)
                     /gene="thrS"
                     /locus_tag="VF870_14430"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9253440.1"
                     /translation="MNKIKITFPDGAVKEFDNGVTGYQIAQSISNRLADEVLAVKFSG
                     AIRDLHRPIKEDGSVKFLTFDDNEGKEVYWHSSSHLMAHAVQEIFPEAKFGVGPAIEA
                     GFYYDIDINTQLNEDHLKLIEDKMLELSKTDTRFEREELSQQEAIEFFKKKGDQYKVE
                     LLSEMDESKEKISIYKEGSFTDLCRGPHLPNPGKIKFVKLLNISGSYWRGDENNKQLQ
                     RIYGITFPKKKMLDEYLFMLEEAKKRDHRKLGKELELFFFSQSVGSGLPIWLPKGAIL
                     RETLERFLRDEQTKRNYLPVITPHIGNINLYKTSGHYPYYSDSQFPTMKFGDREEEYL
                     LKPMNCPHHFQIYASKPRSYRDLPIRYAEFGTVYRYEQSGELNGLTRVRCFSVDDSHM
                     FVRQDQLKDELCNVIDLIQYVFKVMGFADFTTQLSFRDENAEKYGGDLSQWEKAQAEI
                     KEAADIMKLNYEIEKGEAAFYGPKIDFMVKDALGRKWQLGTVQIDYVMPERFNLEYIG
                     SDGQKHRPVVIHRAPFGSLERFIGVLIEHFAGEFPLWLSPTQVVVIPVSQHFLEYGNK
                     VADELKNSGIRLEIDERNEKIGYKIRDWEMKKVPYMLIVGEKEQEAGSVSVRKHKEGD
                     QGSLALSEFIDKIASEIKNKV"
     gene            complement(3683..3756)
                     /locus_tag="VF870_14435"
     tRNA            complement(3683..3756)
                     /locus_tag="VF870_14435"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3720..3722),aa:Val,seq:gac)
     gene            complement(3838..3923)
                     /locus_tag="VF870_14440"
     tRNA            complement(3838..3923)
                     /locus_tag="VF870_14440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3887..3889),aa:Leu,seq:taa)
     gene            4259..4666
                     /gene="mce"
                     /locus_tag="VF870_14445"
     CDS             4259..4666
                     /gene="mce"
                     /locus_tag="VF870_14445"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX9253441.1"
                     /translation="MNISHIEHIGIAVKNIDQAIKFYEDILGLKCYAVEEVADQKVRT
                     AFFMVGQTKIELLESTSPDGPIGKFIEKKGEGIHHLAFAVEGLQNSLDELKSKNVVLI
                     DEKGRKGAEELNIAFLHPKSTFGVLTELCEKPE"
     gene            4716..>5447
                     /locus_tag="VF870_14450"
     CDS             4716..>5447
                     /locus_tag="VF870_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9253442.1"
                     /translation="MAMEDKIKELMSLRQQAKLGGGEKRIESQHKKGKLTARERLELL
                     LDEGSFEEFDMFVCHRSIDFGLDKESYLSDGVVTGYGTIDGRLVYVFSQDFTVFGGSL
                     SEMFAQKICKVMDKALKVGAPVIGINDSGGARIQEGVKSLGGYADIFQRNILASGVIP
                     QISAVFGPCAGGAVYSPALTDFIVMNNSASYMFVTGPKVVKTVTGETVNEDELGGAMV
                     HASKSGVAHFVSEDEEEGISLIRKLL"
BASE COUNT         1705 a         1008 c          842 g         1892 t
ORIGIN      
        1 gtacgaagca aaaaatcttt actaacaaaa aaagtatcct gcatatctct tgccggatga
       61 tctgcgggaa agtttagtga ttcaaaatta taataatcag attctaactc aggtccgttc
      121 gctactgaaa atcctaatcc tttaaatata gattttattt catcaagagt ttgagtaaga
      181 atatgtttac ttccaataat atattcttta ccgggtaaag taaggtctat ttcattttct
      241 ttacttgact gagttgaaga aattctttct tttacttctt caaagtatgt tgaaacctca
      301 ttcctaagaa gatttaactt cttacccagt ataggttttt cttctttaac aacatcttta
      361 agatcttcaa atagttttga aacaagaccg tttctgctga aatattttat ccttatttct
      421 tcaacatcct gtattgatga aacagatttt atatctgagt aaaaactttc ttttatttct
      481 gtaatgtttt tatcaagcat aagatatatt taaatagaaa ataccctcga cggatgtaaa
      541 taaaaacaaa tccgaagagg gtataaaatt agttgataga aaatttaact acttcagaaa
      601 acgctgttgg gttttcagca gctaaactag caagtacttt tctattgata tcaacacctt
      661 ttttattcag tgcgtcaatc aatctggaat aagttgtgcc gtttgttctt gcagcagcat
      721 taattctcac tatccaaagt gatctataaa ctctcttctt aagctttcta tctctgtaag
      781 cgtgtcctaa acctttatcg atagcatttt ttgcagcggt aagaacatta ccttgatttc
      841 cccaataacc ttttgcacgc tttagctgat ttcttcttct tctatgcgaa gcaactttat
      901 tttttgatct tggcattttt tatccttctc tatttttgaa ccattatcct tacacgtttt
      961 tgttcggcat cacttaccaa tgtgcctttt ctcaaacttc tttttctctt tgtagtttta
     1021 gaagtaagaa tatgactttt ataagccttc tttctcttaa aacttcctga tgcagtcttc
     1081 ttaaatgatt tagaagctcc gcggttactt ttcatttttg gcatgttatc cctgcttatt
     1141 tgttttcttt actttttttg attgtggaac caatatggtt gccatgtttc ttccttcaag
     1201 tttgatggga gattctactt tagcaacctc ttccaatttt tcaataaatc gttttaataa
     1261 ttcttcaccc tgatcagtat aagccatttc acgacctttg aacatcacac taaccttaac
     1321 tttatttcct tcttcaagaa aatttattgc gtgtttaact ttaaaatcaa aatcgtgaac
     1381 atcagtattt ggatgcattc taatttcttt cagaatggat acaacctgat tttttttctg
     1441 gatcttatca cgtttttgct gttcatattt gaacttgccg aaatcaataa ttttgcaaac
     1501 tggcggttct gcttgcggag caatctcaac aagatcctgt cccctttctt cagataatct
     1561 aattgcatct ctaacagtgt aaacacctaa ttgcgaacca tctacatcaa ttactcttac
     1621 tttagctgct cttatctctt cattaatacg aactgtttgt ttcccagcga tgcgaacctc
     1681 tcttaattat tattaaactt tgtttttaat ttctgatgca attttatcaa taaattctga
     1741 taaagccaaa gaaccctgat caccttcttt gtgttttcgt acagaaactg aacctgcttc
     1801 ctgttccttt tcacctacaa tcagcatata aggaactttt ttcatctccc aatctcgaat
     1861 cttatatcca attttttcat ttctttcatc aatttccaaa cgaattccag aatttttaag
     1921 ttcatcggca actttatttc catattctaa aaagtgttgt gaaaccggaa taaccacaac
     1981 ttgtgtaggc gacagccaca aaggaaattc acctgcaaaa tgctcaataa gaactccaat
     2041 aaatctttcc aatgaaccaa atggtgcccg atgaataaca acaggcctat gtttctgacc
     2101 atcgctaccg atatattcaa gattgaatct ctcaggcatt acataatcaa tctgaacagt
     2161 accaagctgc cattttctac ctaatgcatc tttaaccata aaatcaattt tgggaccgta
     2221 aaaagctgct tctccctttt caatttcata atttaatttc ataatatcgg ccgcttcttt
     2281 tatctcagcc tgggcttttt cccattgtga taaatctccg ccgtactttt ctgcgttctc
     2341 atctctaaag gatagctgag tagtgaaatc tgcaaagccc attactttaa atacgtattg
     2401 aatcaaatca attacattac acaattcatc ttttaactga tcctgtcgga caaacatatg
     2461 agaatcatca acagaaaaac atcgcaccct tgtcaatcca ttaagttcac cggattgttc
     2521 gtaacgatat acagttccaa actccgcata gcggataggt aaatctctat aacttcttgg
     2581 cttggaagca taaatctgaa aatggtgtgg gcaattcatt ggttttaaaa gatattcttc
     2641 ttctctgtcg ccaaatttca ttgtaggaaa ttgtgagtcg ctataatatg ggtaatgccc
     2701 gctggtttta taaagattta tattccctat atgtggagtg ataacaggca ggtaatttct
     2761 ttttgtctgt tcatctctta aaaatcgctc tagtgtttct cttaaaatag cacctttagg
     2821 taaccagatt ggcagtcccg aaccgacact ttgagaaaag aaaaataatt ccagttcttt
     2881 gccaagttta cgatgatccc ttttctttgc ttcctcaagc ataaaaagat attcatcaag
     2941 cattttcttt ttaggaaaag ttataccata aattctttga agctgcttgt tgttctcatc
     3001 accgcgccag taagaaccgg aaatgtttag aagcttaaca aattttattt taccaggatt
     3061 tggtaaatgc gggccacggc aaagatcggt aaatgatcct tccttataaa tgcttatttt
     3121 ttcttttgat tcatccattt cagaaagaag ctcaacttta tattgatccc ctttcttttt
     3181 aaaaaattct attgcttcct gttgtgataa ttcttctctt tcaaatcttg tatcagtttt
     3241 cgaaagctca agcattttat cttcaataag ttttagatga tcttcattaa gctgagtatt
     3301 tatatctata tcatagtaaa acccagcctc aatagccgga ccaacaccga actttgcttc
     3361 cggaaaaatt tcctgaacag catgagccat caaatgagat gaagaatgcc aataaacttc
     3421 tttaccctca ttatcatcaa aggttaaaaa tttaacactt ccatcttcct taattggtct
     3481 gtgtaaatct ctgattgctc cactaaattt aacagctaat acttcatcag ctaatctatt
     3541 cgaaatagat tgcgcaattt gatatccggt aacaccgtta tcaaattctt ttacagcacc
     3601 atcaggaaat gttattttaa ttttattcat ttaacttttc ataaaaaaaa tcggagaaaa
     3661 ctccgatata ttattcgatt cgtgggctct aaaggagtcg aacctttgac cttctgcacg
     3721 tcaagcagac gctctaacca actgagctaa gagcccaatt caattaacaa ttcataataa
     3781 aaggtgtaca attaagaatt tttaattgca gattatcatt tgttaattaa gttatagtac
     3841 cgagagccgg agtcgaaccg gcacgtccca aacgggacac aggattttaa gtcctgtgcg
     3901 tctaccaatt ccgccatccc ggcaaaccca ttaaaaaaat caaggtagaa tgattttttc
     3961 taattttagc gtgtaaaaat agagaaaatg attttttatt gcaagaatct tctaataact
     4021 ttttttctat gattttagca tattagaaag aatgattagc tttaaatgaa taagctattt
     4081 ctcacaacta agaatttaaa atttctatta ttcttaataa gaattgatta acaattgtca
     4141 atttgatcta ttaacattaa actcaatttc cagtaaataa ttgatgatgg catcaacttt
     4201 gccaaactgt agcgatttta attattttct aaacattaat aatcaataaa taaataatat
     4261 gaacatttca cacatagaac acattgggat tgctgttaaa aatattgatc aagcaattaa
     4321 attttatgaa gacattcttg gtctaaaatg ttacgcagtt gaagaggttg ctgatcaaaa
     4381 agtacgaact gctttcttta tggttgggca aactaaaatt gaattgctcg aatcaacatc
     4441 tccagatgga ccaatcggaa agtttattga aaagaaaggt gaaggaatac atcatcttgc
     4501 atttgcagtt gaaggactac agaactctct cgatgagtta aaatctaaaa atgttgttct
     4561 gatcgatgaa aaaggaagaa aaggtgcaga agagttgaac atcgctttcc ttcatccaaa
     4621 atctacattt ggagttctga ctgaactttg cgaaaaaccg gaatagaaat tattaaactc
     4681 tatactacat aaaatatatt taataggtac acactatggc aatggaagat aaaataaaag
     4741 aattgatgtc gcttcgtcaa caggcaaaac ttggaggcgg agaaaagcgc atcgaatctc
     4801 agcataaaaa aggaaaactc acagctagag aaagactgga acttttactt gatgaaggca
     4861 gctttgaaga atttgatatg tttgtttgtc atcgaagtat tgattttggt ttagataaag
     4921 aatcttattt atcagatgga gttgttactg gttatggaac cattgatgga agattagtct
     4981 atgtgttctc acaagacttt acagtttttg gcggttcatt atctgaaatg tttgcgcaaa
     5041 aaatttgtaa ggttatggat aaagcactta aagtcggtgc accagtaatc ggaattaatg
     5101 atagcggtgg tgcaagaatt caggaaggtg taaaatcact tggcggatat gcagatattt
     5161 tccagcgcaa cattttagca tcaggtgtta ttccacaaat ctctgcagtg tttggtccat
     5221 gtgcaggcgg tgcagtttat tcccctgctc taactgattt tattgttatg aataatagcg
     5281 caagttatat gtttgttacc ggaccaaaag ttgtaaaaac agttactggt gaaactgtaa
     5341 atgaggatga attgggtggg gctatggtac acgcttcaaa atcaggagtg gctcattttg
     5401 tttctgaaga tgaagaagaa ggaatctctt taataagaaa actgcta
//
LOCUS       DASWBY010000795         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_521451, whole genome shotgun sequence.
ACCESSION   DASWBY010000795 DASWBY010000000
VERSION     DASWBY010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521451"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VF870_14455"
     CDS             complement(<1..488)
                     /locus_tag="VF870_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX9253443.1"
                     /translation="MSEHQGGQIKGLPENAYTELKPGEEYKPLMPANTNPQEITVYSV
                     TMGLLMAVLFSAAAAYLGLKIGQVFEAAIPIAIIAVGVSTMLKIKGSLGQNVIIQSIG
                     QNSGLIVAGAIFTIPALYILNLQAEFYQIFLASAFGGVLGMLFLIPFRKYFVAEMHGK
                     FP"
     gene            complement(503..>1678)
                     /locus_tag="VF870_14460"
     CDS             complement(503..>1678)
                     /locus_tag="VF870_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253444.1"
                     /translation="CDADWLTLVQKKTYIRNDQVAAVNVKLDNSKIKEPGLYTTKISA
                     YRDDASKTPEFDMLATVVIPYELNSSNNYQIHWNNQTVNQGIIKRYFVKIPAGQNSMK
                     ITLSRDASSNKYSRCRNILYDNNGIQVGGSKILYSVNNDEKVENYYYDLEPGIYEIDV
                     DGFFLATDPSTYNLGVEFLSIQTVDAKELSSNDKRLEFINYFNETKTYNINAEMLGYQ
                     REYNLLVDGNDTYKMPFTIFKGEGSKEFSFTLSKEDYSKLTDFAFQILDETGKAISKG
                     GLSFRTGGGLSVDMPSDKDSAKFLLEIIPAFASKELNANVEVNEITYFPSPVSVDAKS
                     NGRASLTLYPNNIKYVDFNFAKPELTIPADAEGYGKLYFKSPSTEKTEYELPLNFKF"
BASE COUNT          511 a          307 c          284 g          576 t
ORIGIN      
        1 aaaggaaact tgccatgcat ttctgcaaca aaatattttc taaaaggaat cagaaaaagc
       61 attcctaaca ctccaccaaa agcagaagct aaaaatatct ggtaaaattc cgcctgaaga
      121 tttaaaatgt acaaagcggg aattgtgaag atagcaccgg caactatcaa tccggagttt
      181 tgcccgattg actgaataat cacgttctgg ccgagtgatc cttttatctt aagcatagtt
      241 gaaacaccaa ctgcaataat tgcaatcgga atagcagctt caaatacctg cccgattttt
      301 aaaccgagat aagctgcagc agcagaaaat aaaactgcca tcagcaaacc cattgttaca
      361 gagtaaacag tgatctcctg cggattagta tttgcaggca tcagtggttt gtattcttca
      421 ccgggcttaa gttcagtata agcattctcg ggaagtcctt tgatttgtcc gccttgatgt
      481 tcgctcatat tacgttcctt tattagaatt taaagttaag tggaagttca tattctgttt
      541 tttctgtcga tggtgatttg aaatataatt ttccatatcc ttctgcatca gcaggaatag
      601 ttaattcagg tttagcaaaa ttaaaatcaa catacttgat attatttgga tacaatgtta
      661 aggatgctct gccgttgctt tttgcatcga cagaaactgg agaagggaag tatgttattt
      721 catttacttc aacatttgca tttagctctt tgcttgcaaa tgcgggtata atttctaata
      781 aaaattttgc actgtcttta tcagaaggca tatcaacgct aagacctccg cctgttctga
      841 atgataaacc gcctttgctt attgctttgc ctgtttcatc aagaatttga aaagcaaagt
      901 cagtcaattt tgaataatct tctttgctta acgtaaatga aaactctttt gagccctcgc
      961 ccttaaaaat tgtaaaaggc attttgtatg tgtcattacc atcaactaat aaattatatt
     1021 cacgctgata tccgagcatc tctgcattga tattataagt ttttgtttcg ttaaagtagt
     1081 tgataaattc aagtctttta tcatttgatg aaagttcttt tgcatcaaca gtttgtattg
     1141 aaagaaactc aacgccaaga ttataagtag atggatctgt agctaagaaa aatccgtcaa
     1201 catcaatctc ataaattcct ggttcaagat cataataata attttctact ttttcatcat
     1261 tattaactga gtaaagtatt ttcgatccgc caacctgaat tccattattg tcgtaaagta
     1321 tatttctgca gcgcgaatat ttgtttgatg aagcatctct gcttaatgta attttcatgc
     1381 tgttctgtcc agccggaatt ttcacaaagt atcttttaat tataccctgg ttaactgttt
     1441 gattgttcca atgaatttga tagttattcg atgagtttaa ttcatatgga attactaccg
     1501 ttgccagcat atcaaactcg ggagtcttac ttgcatcatc acgataagcg gaaatttttg
     1561 tcgtgtagag tccgggttct ttaatttttg aattatctag tttaacattt acagcagcca
     1621 cctgatcatt tcttatgtaa gtttttttct gtaccagagt taaccagtca gcatcgca
//
LOCUS       DASWBY010000796         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_628239, whole genome shotgun sequence.
ACCESSION   DASWBY010000796 DASWBY010000000
VERSION     DASWBY010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628239"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VF870_14465"
     CDS             complement(<1..849)
                     /locus_tag="VF870_14465"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9253445.1"
                     /translation="MEKLVTSEIKLGIIAGGQLGKMLVLAASNWNVKSYVLDKDDHCP
                     ASSSCTFFFKGDQLHYADVYRFGQMVDVLTLELENVNIEALKKLKLEGKKIYPDPDKL
                     EIIQDKGLQKQFYDKIKVPSSSFLLFNSKQEILPAVESGKLQLPFVQKLRKGGYDGRG
                     VSIIRSKDDLISLLDGASVVEHLVDIEKEISAIVARNERGEVKCHPTVEMEFNPNANL
                     VEKLICPSTLAKETEEEAEKIAIKIITELDLYGLLAVEMFVDKNKNIWVNEVAPRPHN
                     SGHHTIE"
     gene            complement(849..>1516)
                     /locus_tag="VF870_14470"
     CDS             complement(849..>1516)
                     /locus_tag="VF870_14470"
                     /inference="COORDINATES: protein motif:HMM:NF017765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HEX9253446.1"
                     /translation="AAAYGSVSAVTFVAAVSFLEMQQISYGGHMVAVMALMESPAIVI
                     GVVMMMMFGNESAKGVNWKSIIQHSFTNGSVVMILGSLIIGIIADTKQAEGIKPFTND
                     IFKGFLAIFLLEMGMVAARRFSAFMKYGWFVSLFAIIIPIINGCIVAYISQFVTPDIG
                     NRFILAILSASASYIAVPAAMRIAAPEADPGLYIPMALGITFPVNITIGIPLYFTIVN
                     YFV"
BASE COUNT          487 a          322 c          248 g          459 t
ORIGIN      
        1 ttcgattgta tgatggccac tattgtgcgg acgtggtgca acctcattta cccagatatt
       61 tttattctta tcaacaaaca tttccacagc aagtaatcca taaagatcca actcagtaat
      121 aattttaatt gcaatttttt ctgcttcttc ttcagtctct ttcgctaaag tggagggaca
      181 aattaacttt tcaacaagat tggcgttcgg attaaattcc atttcaactg ttggatggca
      241 tttcacttct ccgcgttcat tgcgggcaac aattgcagat atttcttttt ctatatcaac
      301 cagatgttca acaacagatg caccatcaag caaactgatc agatcatctt tagaacggat
      361 aattgaaaca cctcttccat cgtatcctcc cttgcggagt ttctgaacga atggtaattg
      421 taactttccg gattcaacgg ccggaagaat ttcctgcttg ctattaaaaa gcaagaatga
      481 tgatgaagga acttttattt tatcatagaa ttgtttttgt aatcctttat cctgaataat
      541 ttcaagctta tcgggatcgg ggtaaatttt ttttccttcc agctttagtt tttttagtgc
      601 ttcaatgttc acattttcca attcaagagt aagaacatct accatttggc caaacctgta
      661 aacgtcagcg taatgaagct gatctccctt gaagaaaaac gtacacgaag aagaagcagg
      721 gcaatgatca tctttgtcaa gcacataact tttaacattc caattacttg cggcaagcac
      781 gagcatttta ccaagctgac cgccggcaat tattccaagt ttaatttctg acgttactaa
      841 tttttccatt atacaaaata gtttacgatt gtaaaataaa gaggaatacc aattgtaatg
      901 ttaacgggaa aagtaattcc caaagccatt ggaatataaa gccctggatc agcttctggg
      961 gccgcaatcc tcatagccgc aggaaccgca atgtaagatg cgcttgctga aagtattgca
     1021 agaataaatc tgttgccaat atccggagtt acgaattgac ttatgtatgc aacaatacat
     1081 ccattaataa tcggaataat tatggcgaac aaagaaacaa accaaccata tttcataaat
     1141 gctgaaaatc ttcttgcagc aaccattccc atttccaaaa gaaatattgc aagaaaacct
     1201 ttaaagatat cgttagtaaa aggtttaata ccttcggctt gttttgtatc tgcaatgatc
     1261 ccgattatca agcttcccag aatcataaca acacttccat tggtaaaaga atgctgaatt
     1321 atagatttcc aattcactcc ttttgctgat tcatttccga acatcatcat cataacaacg
     1381 ccaatcacaa tcgctgggga ttccattaat gccatcaccg caaccatgtg tccaccgtaa
     1441 gaaatttgct gcatctccag aaaagaaaca gcagcaacaa aagtaaccgc acttacagaa
     1501 ccatatgctg cggcta
//
LOCUS       DASWBY010000797         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_70655, whole genome shotgun sequence.
ACCESSION   DASWBY010000797 DASWBY010000000
VERSION     DASWBY010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70655"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="VF870_14475"
     CDS             <1..1296
                     /locus_tag="VF870_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX9253447.1"
                     /translation="MHHNGAFSVLMSFNFFKGFGKPRPEPFTKYPPPAEYDSPDSYNF
                     FLDHTPIKKLNEEILKNEIPFWDTMMAHGAYDYYWKSRSNLQYLKNIKPALLLVGGLY
                     DAEDMYGPLHIYKTIEQNDKPNDTRLVMGPWTHGSWIWAKGDSLGDFYFGGNTADYYR
                     KEILLPFFKYYLKGEGDLKLNDAYVFDTGKNEWSNYQAWPPKNGSELKYYFHSDEKLN
                     SQTPTDGKSFTEYISDPCNPVPYTAKFQDSKNFYNPVHLIEDQRYVSTRPDVLTFESG
                     ALDQDITIAGPIIADLFVSTTGTDADFVVKVIDVYPDSAKDPEPNPAKVEMGGYERLV
                     RTEIFRGKFRNSYENPEPFSQNKIAEVKINLNNAFHTFKAGHKIMIQVSSSYFPFFDV
                     NPNTFCDIYSADKDKFVKAMIKVYHDKDYSSSVSFKVIK"
     gene            1435..2424
                     /locus_tag="VF870_14480"
     CDS             1435..2424
                     /locus_tag="VF870_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX9253448.1"
                     /translation="MKKIIIDICLLIVFLSIPISPQSVFGDEPFAHTFSIVARDPQTG
                     EMGVAVQSHWFSVGSLVAWGEAGVGVVATQSFVNPSFGQRGLELLKTGKTAQEVVDAL
                     IASDEGRDVRQLAIVDAKGNSACYTGAKCIPQAGHIVGNNYSVQANMMLNNTVWGAMS
                     KAFENSKGPLAERLIAALEAAQKEGGDIRGKQSACLLVVKGKATGNLWEDRLIDLRVE
                     DNIQPIDEIKRLLKLYRAYEHMNNGDLATEKNDMKLAMDEYNAAMQMFPENLEMKYWT
                     AVSLANTGDADKSLPMFKEIFAKDKNWKELTKRLPAVGLLIVSDDILKKILAQ"
     gene            2537..>2767
                     /locus_tag="VF870_14485"
     CDS             2537..>2767
                     /locus_tag="VF870_14485"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9253449.1"
                     /translation="MLKLSTMFGLMFLFSVITITAQQNLTTPQASQQASVSQRVGLTD
                     IEVKYHRPAVNNRVIWGGLVPYDEVWRAGANEN"
BASE COUNT          908 a          427 c          544 g          888 t
ORIGIN      
        1 atgcaccaca acggagcttt ttctgttttg atgtctttta atttttttaa aggatttggt
       61 aaaccacgac cggaaccgtt cacaaaatat ccaccaccag cagaatatga ttctcctgat
      121 tcttataatt ttttcttaga tcatacacct ataaaaaaac tgaatgaaga aatattgaaa
      181 aatgaaattc ctttttggga tacaatgatg gcacatggag cttatgatta ttattggaaa
      241 tcaagaagca atttgcagta tctgaaaaat atcaaaccgg ctttgctgtt ggttggtggt
      301 ctttatgatg ctgaggatat gtatggtcca ttgcacattt ataaaacaat tgagcaaaat
      361 gataaaccta atgatacaag attagttatg ggtccatgga ctcacggctc atggatttgg
      421 gcaaaaggtg attcacttgg tgatttttat tttggcggca acactgctga ttattatcga
      481 aaagaaattt tattaccttt ctttaagtat tatttaaaag gtgagggaga cttaaaatta
      541 aatgatgcct atgtatttga taccggaaaa aatgagtgga gtaattatca agcctggcct
      601 ccaaaaaatg gtagtgagtt aaaatattat tttcattcgg atgaaaagtt aaattcacaa
      661 accccgactg atggaaaatc ttttactgaa tatattagcg acccatgcaa tccggttcca
      721 tacacagcaa agtttcagga ctcaaaaaac ttttataatc ctgtccattt aattgaagac
      781 caacgatatg tttcaactcg ccctgatgtt ttaacatttg agtctggtgc attagatcaa
      841 gatataacga ttgcaggacc tatcattgct gacctatttg tatctacaac aggaactgat
      901 gctgattttg ttgttaaagt aattgatgtt tatcctgata gtgcaaaaga ccctgaacca
      961 aatcctgcaa aagtcgaaat gggtggatat gaaagattag tccgtacaga aattttccgg
     1021 ggtaagttta gaaatagtta tgaaaatcct gaaccgttta gtcaaaataa aattgccgaa
     1081 gtcaaaataa atttgaataa tgcatttcat acatttaaag caggacacaa aattatgatt
     1141 caggtttcgt catcatattt cccatttttt gatgtgaatc caaatacatt ttgtgatatt
     1201 tactctgcgg ataaagataa gtttgtgaaa gcgatgatta aggtttatca cgataaagat
     1261 tactcaagtt ctgtttcatt taaagttata aagtaatagt ttatataaaa atatttatat
     1321 tggtgttggt cacacttcga cttcgctcag tgtgacttat gtttttgaat tacatatagt
     1381 atcatcctga gcgaagccga aggatgacct tgtttaattt taagaggtgg tgtaatgaaa
     1441 aaaattataa tcgatatttg tttgcttatc gttttcttgt ctatcccaat atctcctcaa
     1501 agtgtttttg gagatgaacc gtttgctcac actttttcaa ttgttgctcg tgatccacaa
     1561 accggagaaa tgggtgtcgc tgttcaatca cactggttct cagttggatc attagttgca
     1621 tggggtgagg ctggagttgg ggttgtggca actcagtcat tcgtaaatcc ttctttcggg
     1681 cagcgagggc ttgaactgct taaaacagga aagactgcac aagaagttgt tgatgcgctt
     1741 attgcatctg acgaagggag agatgtaagg cagcttgcta tagttgatgc caaaggtaat
     1801 tctgcttgtt acacaggtgc aaaatgcatt ccccaagcag ggcatattgt tggaaataat
     1861 tattctgttc aggcaaatat gatgcttaat aatacagttt ggggtgcaat gtccaaagct
     1921 tttgaaaatt caaaaggtcc gttagctgaa agattaattg ctgctttaga agctgctcaa
     1981 aaagaaggcg gtgatattcg aggtaaacaa tctgcctgct tactggttgt aaaagggaag
     2041 gcaacaggaa atctatggga ggatcgatta attgatctta gagttgaaga taatattcag
     2101 ccaattgatg aaataaagag attattaaaa ctttatcgcg cgtatgagca tatgaataat
     2161 ggtgatcttg ctacagaaaa gaatgatatg aaacttgcaa tggatgaata caatgcagca
     2221 atgcaaatgt ttccggaaaa tcttgaaatg aaatactgga ctgctgtttc attagctaac
     2281 accggtgatg cggataaatc tttaccaatg tttaaagaaa tttttgctaa agataaaaac
     2341 tggaaagaat tgactaaaag actgccggca gttggattat taattgttag tgatgacatt
     2401 cttaaaaaaa tacttgcaca ataatttcaa aaatgacttt taatcagttg gattagccgt
     2461 ttatcaatta aaaatattat tgattaaata tcactttatt ttctgatatt catttttcaa
     2521 aaaagagagg catgttatgt taaaactttc cacaatgttc ggattaatgt ttttattttc
     2581 agtaattaca attactgcac agcaaaatct aacaacacca caagccagtc agcaagcttc
     2641 agtttcacaa agagttggac ttacggatat tgaagtgaaa tatcatcgcc ctgccgttaa
     2701 caaccgcgta atttggggtg gtcttgttcc ctatgatgaa gtttggcgcg caggtgcaaa
     2761 tgaaaat
//
LOCUS       DASWBY010000798         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_748017, whole genome shotgun sequence.
ACCESSION   DASWBY010000798 DASWBY010000000
VERSION     DASWBY010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748017"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VF870_14490"
     CDS             complement(<1..704)
                     /locus_tag="VF870_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9253450.1"
                     /translation="MKFLLSFFLSVLIMTTNYSQQEKQTSHSFQNEVKINLSANYLLY
                     LPKDYDDSDKLFPLVLFLHGMGERGSDIEKVKTHGIPKLISEGKEFPFIVVSPQCPDE
                     YLWNDDVLIALLDEIEENYRVDKNRIYVTGLSMGGHGTWTLALAQPNRFAAIAPVCGW
                     GHPVEFCKLAHMPIWVFHGAKDFVVPLSLSEQIVEQLKACGADKVKFTIYPEATHDSW
                     TETYNNEELYKWFLEQS"
     gene            complement(764..1585)
                     /locus_tag="VF870_14495"
     CDS             complement(764..1585)
                     /locus_tag="VF870_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX9253451.1"
                     /translation="MKRKILSKIIIMLFALSSISFTNCSKDDSTTTPDNNVIPPIPGY
                     SLVWNDEFENPDIDLTKWEHEVNGDGGGNNELQYYTDRASNSYIENGSLVIQALKENY
                     QTKEYTSARLRTANKGDWLYGRFDIRAKLPFGQGLWPAIWMLPTDWEYGGWPASGEID
                     IMELLGHQPYKVYGTIHYGTSSNHQSSGASYQLPTSTFSADYHLFTIEWDATGIRWYV
                     DGNKYHTEVHGQPFDKRFHLLLNVAVGGNWPGSPNETTSFPQKMYVDYVRVYKKD"
BASE COUNT          557 a          360 c          312 g          649 t
ORIGIN      
        1 gattgctcaa gaaaccattt gtaaagttct tcgttattat aagtttctgt ccagctatca
       61 tgagttgctt cagggtagat tgtaaattta actttatccg caccgcaagc ttttaattgt
      121 tccacaatct gttctgaaag acttaacggt actacaaaat cttttgctcc gtgaaaaacc
      181 cagataggca tatgcgctaa cttacagaat tcgacaggat gtccccagcc gcaaacagga
      241 gcgatagcag caaaacggtt cggctgtgcc aatgcgagtg tccatgttcc atgaccgccc
      301 atacttaaac ctgttacata aatcctattt ttatctacac gataattttc ctcaatttca
      361 tctaataatg caatcaagac atcatcattc caaaggtatt catcagggca ctgaggcgaa
      421 accacaatga aaggaaattc ttttccttca cttattaact ttggaattcc atgtgttttt
      481 actttctcaa tgtctgatcc tctttctccc ataccatgca gaaaaagtac caatggaaat
      541 agtttgtcac tatcatcgta atcttttggc agatatagaa ggtaatttgc agataaatta
      601 attttaactt cattttgaaa gctatgtgaa gtttgttttt cctgttggga ataatttgtt
      661 gtcattatta aaactgataa aaaaaatgat aatagaaatt tcattatttc tccataatat
      721 aaagaagacg tattttataa taatatcatc ttgaaaagtt taactagtct tttttataga
      781 ctctcacata atcaacatac attttttgag gaaaactagt tgtttcattt gggctgccag
      841 gccagtttcc accgacggcc acatttaata ataaatgaaa ccgtttatcg aatggttggc
      901 catgaacttc agtatgatat ttatttccat caacatacca gcgaataccg gttgcatccc
      961 attcaattgt aaacaggtga taatcggcag aaaatgtact tgttggtaac tgataactgg
     1021 ctccgcttga ctgatgattg gatgaagtgc cataatggat tgttccataa actttatacg
     1081 gttggtgacc aagtaattcc ataatgtcta tttcgccgct tgcgggccat ccgccgtatt
     1141 cccaatcagt tggaagcatc catattgctg gccaaagtcc ttgcccaaaa ggcagcttag
     1201 ctcgaatatc aaatcggcca tagagccaat cacccttatt tgcggttcgt aatctagcag
     1261 aagtgtattc tttggtttga taattttctt ttaatgcctg aataaccaga gaaccatttt
     1321 ctatatacga gtttgaagca cggtcggtat agtattgaag ttcattattt ccaccaccat
     1381 cgccattaac ttcatgttcc cattttgtta aatctatgtc tggattttca aactcatcgt
     1441 tccatactaa agaataaccg gggattggag gaattacatt attatccgga gttgttgttg
     1501 aatcatcctt gctgcagttc gtaaatgaaa ttgagcttag ggcgaatagc attataataa
     1561 ttttagataa tatttttctt ttcatataat gtcctaacag ttttcttcgt catactgagc
     1621 gaagtcgaag tgtgacttta gttatgtccg gtatgatgtc atccttcttt ttcgctcggg
     1681 atgaattttg atcaaatccc ttattgaatt aattcaaatt gattttcttt tagatctgct
     1741 gaatttgttc caacataaac cttaaacatt ccaggttctg atttaaaatt cagatcagcg
     1801 gcagtaaatc ttaaatcctt ttcgtaaata taaaagtcaa ttgttttttc ttcacctcgt
     1861 tttagcatta ctttttta
//
LOCUS       DASWBY010000799         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_70741, whole genome shotgun sequence.
ACCESSION   DASWBY010000799 DASWBY010000000
VERSION     DASWBY010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70741"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VF870_14500"
     CDS             complement(<1..613)
                     /locus_tag="VF870_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX9253452.1"
                     /translation="MKIFLSLIISVLLSLNIYAQSNMEKVNSIIQTVKEEFAPDKRTA
                     IFNIEPAESENKIIIKGETNLAEAKSEFEMKMKEAGLNFEDEIEILPSKKLGDKIYGV
                     INLSVANFRSKPDHPAELVTQSILGTPVKVYKKGEDGYYLVQTPDGYISWLDDDGLTF
                     MNEDQWNEWKSSPKIIYTKEYGFSYSEADVNSQTVSDLVAGNLL"
     gene            complement(627..>1879)
                     /locus_tag="VF870_14505"
     CDS             complement(627..>1879)
                     /locus_tag="VF870_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX9253453.1"
                     /translation="LQFEKERLDRIRKDFNKTAEDVLKVVNKYYPDVKEKGLEKWEKD
                     GSLEYKVIDGKKWYFSRAASNLFRINNEAKAQKEKVDGYQKDGLDAFLEGYIPNVLDE
                     SANSQESLVKPITFKLNYKVTVDANAVPDGEIIKCWLPFPREGHQRQTDIKLLAVNND
                     EYIIASNDNPQRTFYLQKPAIKDQPTIFNMVLEVTNYNEVNFISPDLIKPYNKDSGLF
                     KTYTAERAPHIVFTDKIKKLSKEIVGDEKNPYLIAKKIFTWISDNIPWAGAREYSTIE
                     NISDYCITNMHGDCGIKTLLFMTLCRYNGIPVKWQSGWMLHPGEVNLHDWSEIYLEGY
                     GWVPVDQSFGLIESDQEDEKYFFLGSTDPYHLIVNDDYSKELFPAKTFQRSETVDFQR
                     GELEWRGGNIYFDKWDYHMDVEYK"
BASE COUNT          535 a          355 c          289 g          700 t
ORIGIN      
        1 ttaatagatt tcctgccaca agatcagaga cagtttggga attaacatcg gcttctgaat
       61 aagaaaatcc atattctttt gtgtagataa tttttggcga tgatttccat tcattccact
      121 gatcttcatt cataaaagtt aatccatcat catccagcca ggaaatgtaa ccatccggtg
      181 tttgtactaa gtaatatcca tcttcacctt ttttatagac tttaaccgga gtgcctaata
      241 ttgactgtgt aacaagctca gccggatgat ctggttttga tcgaaagttt gcgacggaaa
      301 gattgattac tccgtaaatt ttatcgccta atttttttga tggaagtatt tcaatttcat
      361 cttcgaaatt tagtcctgcc tctttcatct tcatttcaaa ttctgatttt gcttcagcaa
      421 gattagtttc gcctttgatg ataattttat tttctgattc ggctggttct atgttgaaga
      481 ttgcggttct tttatccgga gcaaattctt cttttaccgt ttgaatgatt gaattaactt
      541 tttccatatt actttgtgca taaatattta gtgacaatag taccgatata attaaactta
      601 aaaaaatttt cataatgtta tccattttat ttgtattcta catccatatg ataatcccat
      661 ttatcaaagt aaatatttcc accgcgccat tctaattcac cgcgctgaaa atcaactgtt
      721 tcacttcttt ggaaagtttt agcgggaaat aattcttttg agtaatcatc attcacaatt
      781 aaatgatagg ggtcagtgct tccaagaaag aaatattttt catcttcctg atctgattct
      841 atcaagccaa aagactgatc gaccggaacc catccataac cttctaaata aatttcactc
      901 cagtcgtgca gatttacttc gccaggatga agcatccagc cgctttgcca ttttacagga
      961 ataccattgt atctgcagag agtcataaat aaaagtgttt taattccgca atcgccatgc
     1021 atgtttgtaa tgcaatagtc tgaaatgttc tcaattgtag agtattctct tgccccggcc
     1081 cacggaatat tgtccgagat ccaagtgaag attttctttg caatcaggta aggatttttt
     1141 tcatctccaa caatttcttt ggaaagtttt ttaatcttat ctgtaaaaac tatatgtggt
     1201 gctctttctg cggtataagt tttaaataat ccggaatctt tattataagg tttaatcaaa
     1261 tctggagaaa taaaatttac ttcgttataa ttggttactt caagaaccat attaaaaatt
     1321 gttggttgat cttttattgc cggtttctgt aaataaaatg ttcgctgagg attatcatta
     1381 cttgcaataa tgtattcatc attattaact gccagcaatt ttatatctgt ctgtctttga
     1441 tgaccttcac gaggaaaggg gagccagcat tttattatct caccgtcagg gacagcattt
     1501 gcatcaacgg taactttata gtttaactta aatgttattg gctttaccaa actttcctga
     1561 gaatttgctg attcatctaa tacatttgga atgtatcctt caagaaaagc atcaagaccg
     1621 tctttctggt atccatccac tttttctttt tgagcttttg cctcattgtt tattctaaaa
     1681 agatttgaag cggcccgaga gaagtaccat ttcttcccat caatcacttt atactccagg
     1741 gatccatcct tttcccattt ttcaaggcct ttttctttta catcaggata gtatttattt
     1801 acaaccttca gaacgtcttc tgcagtttta ttaaaatctt ttctgatacg atctaatctt
     1861 tctttctcga attgaagct
//
LOCUS       DASWBY010000800         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_484744, whole genome shotgun sequence.
ACCESSION   DASWBY010000800 DASWBY010000000
VERSION     DASWBY010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484744"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1819
                     /locus_tag="VF870_14510"
     CDS             164..1819
                     /locus_tag="VF870_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253454.1"
                     /translation="MILKKIFLFLVFSSIAFAQVVYEPLHRDVYKFLSRLSQKSVIIF
                     DDQIRPLSRKFIAQKLIEAAEKSSELTKLEKEELGFYSRDFKLEFDMINNIKIDSSEM
                     TIVGYDAGDRLRLFGYRNNFFSLNLSPILGYKMGSRDNQKLTHFWNGIYTYGYIDKYI
                     GFSFDFRDNTEEGNSIDKHKNFTPVTGVDARTDENIVDYSPNKMEYSEINAVIGTDWS
                     WGSFSIGKGFHEWGYGESGLLVLSQKPPSYGFIQLDIKPVDWLRFNYMHGWLSSDIPD
                     SNSFFYNKAGTISFSFVDKFIATHSLIITPITGLDFAIGESIIYDEELEFLYLIPIMF
                     FRLADHQISNQTNAAGGNAQMFLSVSSKGQIKNTHLYGTLFIDEITLSGLFDSYKQRN
                     QLGFTLGGSITDLPIENLTAKIEYTKIYPFVYQHYIQSSDYKSSNYILGHWMGHNADL
                     IYASLNYRFLRGLQATAWGQYIRKGEDGNVEQQYYIQPQPPFLFGLRKNYTYFGAEIK
                     YEYTHELFAKFNFQTTLSSIQQEDLSFIDNRLSEFYFSIYYGL"
BASE COUNT          632 a          272 c          332 g          631 t
ORIGIN      
        1 tacaggaatg ttttaaggat cttggacaca agaaaggcga tttccctatt tcagaatttt
       61 cggcagatac atcaatcgct ttacctattt atccggaact tagcgatgaa cagctaattt
      121 atattgttga aacttttaaa aagttcttta atcaataagt taaatgattt taaaaaaaat
      181 atttctgttt cttgttttta gctccattgc atttgcgcag gttgtttatg aacctttaca
      241 cagagatgtt tataaattcc tctcaaggct tagtcaaaaa tctgtaatca tttttgatga
      301 ccagattaga cccctaagta gaaaatttat cgcacaaaaa cttattgaag cagcagaaaa
      361 atcttctgaa ttaaccaagc tggaaaaaga agagcttggg ttttatagca gggattttaa
      421 attagaattt gatatgatta ataatatcaa aatagactcc tcagaaatga caatagttgg
      481 ttatgatgca ggagatcgac taagattatt tggctatcga aataattttt tcagcttgaa
      541 tttaagtcca attttaggat ataaaatggg ttcccgtgat aaccagaagc taacacattt
      601 ctggaatgga atttacactt atggctacat tgataaatat attggcttta gttttgattt
      661 cagggataac acagaagagg gaaattctat tgataagcat aagaatttta ctccggtaac
      721 tggcgtcgat gcacgtacgg atgagaatat tgtagattat tccccaaata aaatggaata
      781 cagcgaaata aatgctgtca taggaactga ttggagctgg ggatcatttt ctattggaaa
      841 aggatttcat gaatggggtt acggtgaaag cgggctgctc gtgctttcgc aaaaaccgcc
      901 atcttatgga tttatacaac tggatattaa gccggtagat tggctaagat ttaattatat
      961 gcatggctgg ctttcttcag atatacctga ttcaaactct tttttttata ataaagccgg
     1021 aacaattagt ttttcttttg ttgataaatt tattgctacc cactctttga ttattactcc
     1081 gataactggt ttggattttg caattggaga atcaattatt tatgatgaag aacttgagtt
     1141 tttatactta ataccaatta tgttttttag attagcagat catcagataa gcaatcaaac
     1201 aaatgctgct ggtggaaatg cgcaaatgtt tttaagtgta agctcaaaag gacaaattaa
     1261 aaacactcat ctatatggaa ctctatttat tgatgaaata acattaagtg gattatttga
     1321 ttcttataaa caaaggaatc aactcgggtt tactctcggt ggctcaatta ctgatcttcc
     1381 tattgaaaat cttactgcaa aaatagagta tactaaaata tatccctttg tttatcagca
     1441 ttatatacaa tcctctgatt ataaaagctc aaactatatt cttggacact ggatgggaca
     1501 caatgctgac ctgatttatg cttctctaaa ttaccgattt ttgagaggat tgcaggcaac
     1561 agcttgggga cagtatataa gaaaaggtga agatggaaat gtggaacagc agtattatat
     1621 ccagccacaa ccaccgtttt tatttggctt aagaaaaaac tatacatact ttggtgcaga
     1681 aattaagtac gagtatacac acgaactttt tgctaaattt aattttcaga caacactttc
     1741 ttcaatacaa caggaagatt taagttttat tgataatagg ttgagtgaat tttatttttc
     1801 aatctattat gggttgtaat tttaatgaat gatttaagaa acaaaaaagt ttatttagca
     1861 ggtcata
//
LOCUS       DASWBY010000801         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_521871, whole genome shotgun sequence.
ACCESSION   DASWBY010000801 DASWBY010000000
VERSION     DASWBY010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521871"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="VF870_14515"
     CDS             <1..1183
                     /locus_tag="VF870_14515"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253455.1"
                     /translation="DETTLSYMLYSLSNALKNQSYDKKKYVEIINILLRKDLKSYPLS
                     LLAITLKNMNENQKAKDIAARLKKQVNEEKSFAFWGGEEWHYRWQNDNVQGTAFAVKA
                     ILNVEGNSDLIAKAVRWLLLKRQGYSWRSTQETSVVLFALTDYLKITKELDPDYSVKI
                     FLNDKEVFNKRFDKSDVFKQSENLKFSGSEMNTLKKGNNKLRLEKSGKGILYLSGLSE
                     YFTEDIKSVENNNGFKLRREYYVLETGEKDGHIIYFKKKFDGTVTNGQDIFVKTFIES
                     KSDNLEYFILEDMMPSGFEVVKDMDKYFIDGENNYQTYYDYYYDYMPWRWQYADREYR
                     DEKVAFFVTSCQNKMEFSYIIKAQIPGEYKIMPAQGYLMYYPELNGFSDVVNIKVIDM
                     K"
     gene            complement(1243..>1578)
                     /locus_tag="VF870_14520"
     CDS             complement(1243..>1578)
                     /locus_tag="VF870_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9253456.1"
                     /translation="DEITRLFTNLISNAIKYNRDQGIINIRLYQAENYLITEIKDSGI
                     GLKPEEKQKLFTEFFRAKNDKTKNISGTGLGLSIVKRIVDSYSGKIEVESDYGEGTTF
                     KVFLPKAFN"
BASE COUNT          547 a          214 c          291 g          526 t
ORIGIN      
        1 tgatgaaacg actttatctt acatgcttta ctcattaagt aatgctctaa aaaatcaaag
       61 ttatgataag aagaaatatg ttgaaatcat taatattctt cttcgtaaag atttaaaatc
      121 ttatccactt tctttacttg caattacatt aaagaatatg aacgaaaacc aaaaagcaaa
      181 agatattgct gcaagattaa aaaaacaggt caatgaagaa aaatcatttg cattctgggg
      241 cggggaagaa tggcattaca gatggcagaa tgataatgtg cagggaactg catttgcagt
      301 taaggcaatc ttaaatgtag aaggcaattc ggatttaatt gcaaaagcgg ttagatggct
      361 gcttcttaaa aggcaaggtt attcttggag atcaactcag gaaacttctg ttgttctgtt
      421 tgcattgacc gattatctga agatcacgaa agagctggat cctgattact cagttaaaat
      481 tttcttaaac gataaagaag tatttaataa acgatttgat aaatcagatg tatttaaaca
      541 atctgaaaat ctaaagttca gtggaagtga aatgaatact ttgaagaagg gaaataataa
      601 actaagactt gaaaaatccg gtaaaggaat tttatatcta tcaggcttga gcgaatattt
      661 tacagaagat ataaaatcgg ttgaaaataa taatggattt aagttaagac gcgaatatta
      721 tgttttagaa acgggcgaaa aagacggtca cattatttac tttaagaaaa agtttgatgg
      781 gaccgtaaca aacggacagg atatttttgt aaaaactttt attgaatcaa aatctgataa
      841 tctggaatat tttattcttg aagatatgat gccatcagga tttgaagttg tgaaggacat
      901 ggataaatat tttatcgatg gtgaaaacaa ttaccagact tactacgatt actattatga
      961 ttatatgccg tggcgctggc aatatgcgga tcgtgaatac cgcgatgaaa aagttgcttt
     1021 ctttgttaca agctgccaga ataaaatgga attttcatat atcatcaaag cgcaaatccc
     1081 cggagagtat aaaattatgc ctgcgcaagg ttatttaatg tattatcctg aactaaatgg
     1141 ttttagtgac gttgtgaata ttaaagttat agatatgaaa tagtctttgg ttgaagttag
     1201 gactgagaaa taagagttta aattgatttt ttattagata ttctaattaa atgcctttgg
     1261 taaaaatact ttaaaagttg ttccttcgcc atagtcagat tcaacttcta ttttgccgga
     1321 gtaagaatca acaattcttt taactatgga tagtcctaat cctgtgccgc ttatgttctt
     1381 ggtcttatca ttttttgcac ggaagaattc tgtaaagagt ttttgctttt cttccggctt
     1441 taaaccgatt ccgctgtctt taatctctgt aataagataa ttttcagcct gatatagcct
     1501 aatgtttatt atgccctgat cacggttata ctttattgca ttgctgataa ggtttgtaaa
     1561 taatcttgtt atttcatc
//
LOCUS       DASWBY010000802         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_521874, whole genome shotgun sequence.
ACCESSION   DASWBY010000802 DASWBY010000000
VERSION     DASWBY010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521874"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VF870_14525"
     CDS             <1..408
                     /locus_tag="VF870_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253457.1"
                     /translation="SVNYLLSKKRLLKFFKEAKNHLTENGVLTFDAALESNSYKHQKT
                     ATTKGKAKGFKYHRKSIYLPKSKIHKNIFTIFYPNGETFSETHKQKIYSYDTFFEMAE
                     KCGLYVVNCYKAFSHTRGKATSDRAQFIMKRKS"
     gene            408..1082
                     /locus_tag="VF870_14530"
     CDS             408..1082
                     /locus_tag="VF870_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9253458.1"
                     /translation="MLSIQDISFNYENQTLFENANLELAAGEFAFLIGKSGSGKTSLL
                     QMIYMNLLPQSGTVTVGKFNSKTIKKSELPLLRRKIGIVFQDFKLLTDRNVFENLSFV
                     LEVIATPKKEIKKKVNAVLNEVGLSHKRLNNPENLSGGEKQRVAIARAILNDPIIILA
                     DEPTGNLDPETSGEILDLLQKINKRGTAVLFATHNYDMVRKGNTRIFKIEDKKILKGF
                     LKQKDQ"
     gene            complement(1076..1735)
                     /locus_tag="VF870_14535"
     CDS             complement(1076..1735)
                     /locus_tag="VF870_14535"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX9253459.1"
                     /translation="MRLILFGSPGVGKGTQAKIISKNLNIPHISTGDILRKAVKDQTE
                     LGKRAGELMSQGELVPDDLMIGMIKEVLTSDECSHGFILDGFPRTKVQAEALDKLFEQ
                     IGIDNAILIHITADQNEIIKRLNNRRACKECGNIFNLKEIDGLDSCPNCGAKNSFYLR
                     NDDQEDVIKNRLDIFNSSTMPVLGYYENKDRVIVVNGFDTVENVNKKIIESLKVKNLY
                     Y"
     gene            complement(1808..2281)
                     /gene="tadA"
                     /locus_tag="VF870_14540"
     CDS             complement(1808..2281)
                     /gene="tadA"
                     /locus_tag="VF870_14540"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX9253460.1"
                     /translation="MLFSEDSYRYMYAALQEAQKAFDDDEVPVGAVVVHKNRIIGRGY
                     NQVEKLKDATAHAEMIAITSASNNLQGWRLNECSIYVTLEPCIMCTGALLFSRISNLF
                     FAASDSKFGACGSIHNLAEYSKTNHKIKVYSGILAKESEEMLKSFFAKKRLASKK"
     gene            complement(2509..2582)
                     /locus_tag="VF870_14545"
     tRNA            complement(2509..2582)
                     /locus_tag="VF870_14545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2546..2548),aa:Arg,seq:tcg)
     gene            2704..>3116
                     /locus_tag="VF870_14550"
     CDS             2704..>3116
                     /locus_tag="VF870_14550"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009095 - aromatic amino acid family
                     biosynthetic process, prephenate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX9253461.1"
                     /translation="MKEIREKINTIDDKMLQLLAERRELSVEIIKLKNEDKSAIRDRD
                     REKQLLIRLIEAGRNYGLDTHYVSKIFQEIISDSVRIQNRFVLNSSNESEKDDLIKVA
                     IQGIEGSYSYLTTKQFFSDSGKDIHFKKYNTFDEVV"
BASE COUNT         1087 a          517 c          519 g          993 t
ORIGIN      
        1 agtgtaaatt atttactaag taagaaacga ttactaaagt tttttaagga ggcaaaaaat
       61 caccttacag agaatggagt attaactttt gatgccgctc ttgaaagcaa tagttataaa
      121 catcaaaaga ctgcaacaac aaaaggtaaa gcaaaaggtt ttaagtatca cagaaaaagt
      181 atttacttgc cgaagagtaa aatccataag aacattttta caatttttta tccaaacgga
      241 gaaacttttt ctgaaacaca taagcagaaa atttactctt acgatacttt ttttgagatg
      301 gcagaaaaat gtgggttata tgttgtaaat tgttataaag ctttttcgca taccagagga
      361 aaagcaactt ctgatcgtgc tcaatttatt atgaagagga aatcttaatg ttatctattc
      421 aggatattag ttttaattat gaaaatcaga cgttatttga aaatgctaat ctggaattag
      481 cagcagggga gtttgctttt ttaattggaa aaagtggttc aggtaaaaca tctctattac
      541 agatgatcta tatgaacttg cttcctcaat caggaacagt aacggtgggc aaattcaatt
      601 ccaaaacgat aaagaaaagt gagcttccgc tattgagaag aaaaattgga attgtgtttc
      661 aggattttaa attacttact gatagaaatg tttttgaaaa cctttccttt gtgcttgaag
      721 taattgcgac accaaaaaaa gaaattaaga aaaaagtaaa cgctgtactt aatgaagtcg
      781 gcttatcgca caaaagattg aataatcctg agaatctttc cgggggggaa aaacagcgag
      841 tcgcaattgc acgcgcaata ttaaatgatc caataattat tcttgcggat gaacccacgg
      901 gtaatcttga ccctgaaaca tccggtgaaa tattggatct gctgcaaaaa attaataagc
      961 gtggaacagc agttttattt gcgactcaca attacgatat ggtaagaaag ggaaatacca
     1021 ggatatttaa gatcgaggat aaaaaaattc ttaaaggatt tttgaaacag aaagatcagt
     1081 aataaaggtt ttttactttt agtgactcaa taattttttt atttacgttc tcaacagtat
     1141 caaatccatt taccactata actctgtctt tgttctcgta atatcccaga acaggcattg
     1201 ttgatgaatt aaaaatatcc agtctgtttt ttataacatc ttcctggtca tcatttctta
     1261 aataaaaact gtttttcgca ccgcaatttg gacatgaatc cagaccgtca atctctttta
     1321 aattaaaaat gttaccgcac tctttacaag ctctgcgatt attcaatctt tttataattt
     1381 cgttttggtc tgcagtaatg tggataagaa ttgcgttatc tatcccaatt tgctcaaaca
     1441 atttatctaa agcctcagct tgtactttag ttctcggaaa tccatctaga ataaatccat
     1501 gactacattc atctgaagtc agcacttctt ttatcattcc aatcattaaa tcatcaggaa
     1561 caagttcacc ctgagacatt aattcgccgg ctcttttccc cagttctgtt tgatctttta
     1621 cagctttgcg caaaatatca ccggttgaga tatgcgggat atttaaattt ttagatatga
     1681 tttttgcctg ggtacctttg ccaacccctg gagaaccgaa tagaattaag cgcatataat
     1741 cttcattttt ttattgcaaa ttagaagtaa atggtttctt aagcaagaaa ttattacctc
     1801 gtaagaatta ttttttagat gcaagccgct tttttgcaaa gaatgatttt agcatctctt
     1861 cactctcttt ggcaaggatc cctgaataca cttttatttt atgattcgtc tttgaatatt
     1921 ctgccaaatt atggatactg ccgcacgcac caaacttgga atcacttgca gcaaaaaata
     1981 aattgctgat tcttgaaaac aatagagcgc ctgtacacat tatgcaaggt tcaagagtaa
     2041 cataaattga acattcattc aatcgccagc cttgtagatt gtttgatgct gaagtaattg
     2101 cgatcatttc tgcgtgagca gttgcatctt ttagcttttc aacctggtta taaccgcggc
     2161 caataattct gtttttatga acaaccacag caccgacagg aacctcatca tcatcaaaag
     2221 ccttttgtgc ctcttgaaga gctgcataca tatatcgata tgaatcttct gaaaaaagca
     2281 taggctaatg caaatttaaa tcaattataa attcatttac tgttaactat ctatctaaac
     2341 taaaagctta taagatattt ttaattcttc agtctgcggg gagccgaaga ctgataagct
     2401 aaaaaattca cacttcgact acgctcagtg tgacaaaatc taagcttttg taaaacaaaa
     2461 atgtacaatc aagttttact cttaattgta cattcttaat tgaaaaagta cgcccggaag
     2521 gattcgaacc ctcaaccctt tgatccgaag tcaaatgctc tatccagttg agctacgggc
     2581 gcgtggtgca aaattacaat taagtataat ttgttcaaaa ctgtactcaa actaatcttg
     2641 attaccagcc aaaataaagc gatattcgag cccgcaatta atatttaaat taaagattta
     2701 gtgatgaaag aaatcagaga gaaaataaat actattgatg ataaaatgct tcaacttctt
     2761 gctgaaagaa gagaattaag tgttgagatc atcaaactga aaaacgaaga taaatctgca
     2821 atacgagatc gtgacaggga aaaacaatta ttgataagac taattgaagc tggaagaaac
     2881 tatggtttgg atactcatta tgtttccaaa atatttcagg aaattataag tgattccgtt
     2941 cgtatacaaa atagatttgt gcttaatagt tcaaacgaat ctgaaaaaga tgatttaatt
     3001 aaagttgcaa tacaaggaat tgaaggctct tacagttact taacaaccaa gcagtttttt
     3061 agtgattcag gaaaagatat tcattttaaa aagtataata cttttgatga agttgt
//
LOCUS       DASWBY010000803         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_33977, whole genome shotgun sequence.
ACCESSION   DASWBY010000803 DASWBY010000000
VERSION     DASWBY010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33977"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VF870_14555"
     CDS             complement(<1..1019)
                     /locus_tag="VF870_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9253462.1"
                     /translation="MKQIALSPFNNIKKILLLLSLFSIVLSAQPKIYTIDEAIGQALR
                     NNKNISISQMDVEKAGATVKEAFGYALPKLDLSGNFSHFIKKPLMPFPDFEALLTNAT
                     YSILFDEGVIPRDESKYLPMETKLQSFALTNAYTTDLTLTQTLFSSAVFEGIGASKIY
                     YNLSKAELDNTVSKTVLDVQKAFYGVILSKKVLEIAQASFKNAQDNFANVLALYKQGM
                     VSEFDKLQAEVQVENIRPSVLQMENTLTNTKNRLKILLGEKQEQDIDVSAEIVYEPIE
                     NIDEEALIDEALQNNFDIKSLSLKKEVDHAFIQLDVSEYWPNIAAFGNYAYSGSSDQW
                     NFQNYS"
     gene            1372..>1584
                     /locus_tag="VF870_14560"
     CDS             1372..>1584
                     /locus_tag="VF870_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HEX9253463.1"
                     /translation="MDQYTAEKLKSIVGAPNFFDSKEDKLVYSYDGTPVYQQLPEAVL
                     FPQDEEQISKILKLANQEKFNVVPRGS"
BASE COUNT          506 a          256 c          259 g          563 t
ORIGIN      
        1 gagtagtttt gaaaattcca ttggtcagat gatccgctgt atgcataatt tccaaaagct
       61 gctatgttcg gccaatattc tgaaacgtct aactgaatga atgcatgatc aacttctttt
      121 tttaatgata ggctttttat atcgaaatta ttttgaagtg cttcatcgat caatgcttct
      181 tcgtcaatat tctcgattgg ttcgtacaca atttctgctg acacatcaat atcctgctcc
      241 tgtttttcac caagcagaat ctttaatctg ttctttgtat tagttaaagt attttccatc
      301 tgaagtacgg atggccggat attttctacc tgaacttcag cctgaagttt atcgaactca
      361 gacaccatac cttgtttata taatgcaaga acattagcaa aattgtcctg agcgttctta
      421 aaacttgcct gtgcaatttc cagcactttt ttagatagta ttacaccgta gaatgccttc
      481 tggacatcta agacagtttt ggatactgta ttatcaagtt cggctttaga taaattgtag
      541 taaatttttg aggcgccaat tccctcaaac actgctgaac tgaaaagtgt ctgggttaat
      601 gtaaggtctg ttgtatatgc atttgttaat gcaaaagact gcagcttggt ttccattggt
      661 aaatatttgc tctcatccct tggtataact ccctcatcaa acagaattga ataagttgca
      721 tttgttaata atgcttcaaa atcgggaaaa ggcattaatg gttttttaat gaaatgagaa
      781 aaatttcctg aaagatcaag ctttggcaaa gcgtaaccaa atgcttcctt tactgtggcg
      841 ccggcctttt caacatccat ctgcgagata cttatatttt tgttattcct tagtgcctgt
      901 cctattgctt catctattgt atagattttt ggttgagcag ataacacaat ggagaataag
      961 gataagagta aaagtatttt tttaatgtta ttgaaagggg ataaggctat ttgtttcatt
     1021 taattctcta aataattgaa cttagaatag actgtttaag atagttttat aaaaatgttt
     1081 aattctttta aatactaaaa attttttaat gaacaaatct aattaactat caaataaaaa
     1141 tgattccgtt gatcacaata ataagcgatt gtgacaagaa tcaacgtttt tgcattggca
     1201 acggttatta tttggcaagt tatttttctc aaaatttatg actcaatttt tttgaccact
     1261 taaaatttat tagaaaatat atttaattaa taccgatctg atatttataa gtatttaaat
     1321 actaaagatt tagaaaaatg agtaactttg tatagtaaaa acaggattgt tatggatcaa
     1381 tacacagcag aaaaacttaa atcaattgtt ggagcaccaa acttttttga ttccaaagaa
     1441 gataaattag tttattctta tgatggcaca cctgtttatc agcagctccc tgaagcggtg
     1501 ttattcccgc aggatgagga acaaatttct aaaatcttaa aacttgcaaa ccaggaaaaa
     1561 tttaatgttg ttccccgcgg ctca
//
LOCUS       DASWBY010000804         4020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_260855, whole genome shotgun sequence.
ACCESSION   DASWBY010000804 DASWBY010000000
VERSION     DASWBY010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260855"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..643)
                     /locus_tag="VF870_14565"
     CDS             complement(113..643)
                     /locus_tag="VF870_14565"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9253464.1"
                     /translation="MVKMLFVLLIALSVNTFSFDKNEINTKSNNAEYAVIKIDDNKAP
                     DFTLKSFDGKTVKLSDYKGKVVIIDFWATWCPPCRKGIPDLISIQNEYKNDVVVIGIS
                     LDSEKTLKDVPGFIKSYGINYPVVYGDEKVVSAYGGIEGIPTAFVVDKKGNVVDKHVG
                     LVPKDTYVNKIKDLLK"
     gene            complement(671..889)
                     /locus_tag="VF870_14570"
     CDS             complement(671..889)
                     /locus_tag="VF870_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253465.1"
                     /translation="MAKKNNTNRERKIGKQVIEKKPAMDPKLKSTIWTIITIVVLLIF
                     FIVNNTRNEPKEGPYPPNYVPSQSNTGK"
     gene            complement(998..2509)
                     /locus_tag="VF870_14575"
     CDS             complement(998..2509)
                     /locus_tag="VF870_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9253466.1"
                     /translation="MKKKSTNYPRLLEINARIWIKRFGDYCKLGSVPDEQINKWKELG
                     FDMIWLMGVWDNNKSVINEYCFEPELISSYNSALKDWSNEDVIGSPYSIDKYELNPLL
                     GNREDLLSFKKKLNDAGIKLILDFVCNHFSAKSSLIWTNKEIFLPADEFIFKNDPYTF
                     FQSPANNKEYLAHGRDPLFPPWKDTAQINFYSREARNYLTSVLIELTELCDGVRCDMA
                     MLPLNNIFYNTWIGVLKKYGYEKPEKEFWEEAISQVKIKRDDFIFIAETYWDLEWQLQ
                     NLGFDFTYDKRLTDRLIDGSIKSIKDHLSAEKDYRDKSVRFLENHDEDRAIVKLGREK
                     SMAAAVITSTIPGVAFYFDGQCDGKRIKLPVQLGREPEEKQDEKIKEFYRNLFRITKS
                     NIFRKGNWKLLESNPVADSDLSCENLLSWEWRLDNELRVVVVNYNNSTSRCRLKFNIQ
                     SNSDDIVLTDLVNSVTYKRSVKEIAEKGLFIELKAFNSHIFSFNENGSDQSIH"
     gene            2955..3938
                     /locus_tag="VF870_14580"
     CDS             2955..3938
                     /locus_tag="VF870_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253467.1"
                     /translation="MSITTLKQKDNTTKNLLNPDVANNELELELEEYTRSAVRNIQFT
                     VILKPAIISFIALFISFFLDLSSVPILGNVTTDLAKSLFPSWQPVKESVVPFSFWWLP
                     VVTYALFIFLAFLAYSKLKLEIIRTPASATIDRVIGSYNSVIDSIATALPLIGAAILL
                     ISIKLGEEIFLGLSVPFEVKALIVLAIGKLFEPVLDQLGMEFQAVVNHVSDIRDKYFS
                     RIQVENSKNILKQLNQGGSGAGGTELSLADVEKFKMLVEQSAKLSEIMNRNFTSISAL
                     ADKVNQIEGVSSQKIIELSGLANALNQASQSLGDEKTLTGLKHLEAIVLKK"
BASE COUNT         1335 a          665 c          587 g         1433 t
ORIGIN      
        1 ttttaagaag cggcttgtaa gattttttgc ataacgaaac ggatcaaaaa aaatccttat
       61 tcaaaaagtg aataaggatt ttttaattta aatgtattca gtgttgtagt tgttacttta
      121 agagatcttt aattttattt acataagtat cttttggaac aagtcctacg tgtttatcaa
      181 caacatttcc ttttttatcg actacaaatg ctgtcgggat tccttctatt ccaccataag
      241 cactaacaac tttttcatct ccatatacaa ccggataatt gattccgtaa ctttttataa
      301 atccaggaac atcttttaat gttttttcac tatccagcga aataccgatt acaactacat
      361 catttttgta ttcattctga atggagataa gatcaggaat tccttttcta caaggaggac
      421 accaggttgc ccaaaaatct ataattacta ctttaccttt gtagtcagat agttttactg
      481 tctttccatc aaatgattta agagtaaaat caggtgcttt attatcatca atttttataa
      541 ctgcatattc agcattgtta gatttagtat taatctcatt tttatcaaaa ctaaatgtgt
      601 ttacacttaa agctatcagt aatacaaata acatttttac cattactatc atttcctttc
      661 tgattaagat ttatttaccc gtgttacttt gtgagggtac ataattcggt ggataaggac
      721 cttctttggg ttcattcctt gtattgttta caataaaaaa tattagtaga acaacaattg
      781 taattattgt ccagattgta ctttttaatt ttggatccat agcaggtttt ttttcaataa
      841 cctgcttacc gatttttctt tcacgatttg tattgttttt ttttgccata attcaatccg
      901 tatgatgcct aaaatatttt ttggtttcgt taacttaaaa atgaaagttt ataattaaaa
      961 gaaaatataa ggtagaaatt attcccagcc tcttttacta atgtattgat tgatctgatc
     1021 cgttttcatt aaatgaaaaa atgtgactat taaatgcttt gagctcaata aataatcctt
     1081 tttccgcaat ctcttttact gagcgtttat aagttacact atttacaaga tccgtcaata
     1141 ctatatcatc agaatttgat tgtatattaa attttaatct gcatcttgat gttgaattgt
     1201 tatagttaac aacaacaacc cgtaattcat tatcaaggcg ccactcccat gaaagtaaat
     1261 tttcgcagct taaatcagaa tcagcgaccg gatttgattc cagtaatttc cagttgcctt
     1321 ttctaaaaat atttgatttc gtaatcctga ataaattacg atagaattct tttatttttt
     1381 catcctgttt ttcttcaggt tccctgccga gttgaacagg cagtttaatt cttttaccat
     1441 cgcactgtcc atcaaagtaa aaggcaacac cgggaattgt acttgtaata actgctgcgg
     1501 ccatggattt ttccctgcca agttttacaa ttgcacgatc ttcatcatga ttctctaaaa
     1561 atctaaccga cttatcccga taatcctttt cagcacttag atgatcttta atacttttta
     1621 tactcccatc gataagtcta tcagttaatc gtttatcata tgtaaaatca aatccaagat
     1681 tctgcaattg ccactcaaga tcccaataag tttctgcaat aaaaataaaa tcatctcttt
     1741 taatttttac ctgagaaatc gcttcttccc aaaattcttt ttccggtttt tcatatccat
     1801 attttttaag aacgcctatc caggtattat aaaaaatatt atttaacgga agcattgcca
     1861 tatcgcatct cactccatca cataattcgg taagttcaat caaaaccgaa gtaagataat
     1921 tacgcgcttc tctgctgtaa aaatttatct gcgctgtatc tttccagggt ggaaacaatg
     1981 ggtcacgtcc atgtgcaaga tattctttat tgttagctgg ggactggaaa aatgtatagg
     2041 gatcattttt aaaaataaat tcatctgcag gtaaaaatat ttctttgtta gtccagatga
     2101 gtgaagattt agcactgaag tgattgcaca caaaatcaag tataagtttt attcctgcat
     2161 cgtttaattt tttcttaaat gacagcagat cttccctgtt tccaagtaaa gggtttaatt
     2221 catacttatc tatagaatat ggtgaaccaa ttacatcttc attagaccag tcttttaatg
     2281 cactgttata ggaagaaata agttcaggtt caaaacaata ttcatttata actgatttgt
     2341 tattatccca aacccccatc aaccagatca tatcaaaacc aagctctttc cacttgttta
     2401 tctgctcatc tggtactgat cctaatttac aataatctcc aaatcgtttt atccaaattc
     2461 gtgcatttat ctcaagcaat cttgggtaat tagtgctttt tttcttcatt cctgacgatt
     2521 attttaaaac aaaatattaa tctttaagca aagataaata taatattgat tgcatttcat
     2581 tgtaatttat aatgatgatg agattaatat ttcaggtaat aattacagaa ttgtaaagaa
     2641 tttttaacaa attgcagaat gatttactac ttcagttaat attaaattta catgagtaat
     2701 ataattgtct ggtaattcaa tatttttaag cttaaaatta taatgttaat gttaaatagc
     2761 ttaatatttg attgaaaacc aagtattttt tctactaaaa gagattttct attacaagca
     2821 taattcccaa acctaaaaca atataaggaa cagtggtatg ataattgtcc caaataagaa
     2881 ccgttttggt taattaaaaa taactatgtg ctttttatac ataaaagttt taccaagaaa
     2941 atttgaggag actgatgtca attacaaccc taaaacaaaa agataataca actaaaaatc
     3001 ttttgaatcc cgatgtagca aacaatgaac tagaactaga acttgaagaa tatacgcggt
     3061 ctgcggtaag aaatattcag tttacggtaa tattaaagcc cgccattata tcatttattg
     3121 cgctttttat ttcctttttt cttgatcttt caagtgtacc gattttaggt aacgtaacca
     3181 ctgatctggc aaaatcactt tttccgagct ggcagccagt aaaggaatca gttgttccat
     3241 tcagcttctg gtggctgcct gttgttactt atgctctgtt tatctttctt gcttttctag
     3301 cctacagcaa attgaaactc gaaattataa gaacaccggc aagtgcaaca atagacagag
     3361 taattggatc ctataacagt gtaattgata gtattgcaac tgcattacct ttaatcggtg
     3421 ctgcgatatt acttataagc attaaacttg gagaagaaat atttcttggc ttgtctgtac
     3481 cgtttgaggt aaaagcttta attgtgcttg caatcggaaa attatttgaa ccggtactag
     3541 atcaacttgg aatggaattt caggcagtag ttaatcatgt ttctgatata agagataaat
     3601 atttttcaag aatacaagta gaaaattcaa aaaatatttt gaagcaattg aatcaaggcg
     3661 gctcgggtgc aggtggaact gaattatcac ttgcagatgt tgaaaagttc aagatgcttg
     3721 tagaacaatc tgcaaagctt agcgaaatta tgaacaggaa ctttacatca ataagtgcac
     3781 ttgcggataa agttaatcag attgaaggag taagttccca aaaaattatt gagcttagtg
     3841 gattagcaaa tgcattaaat caggcttcac aatcactggg tgacgaaaaa acattaaccg
     3901 gattaaaaca cctggaagca atagtattaa agaaataaaa agattttaaa atgataaata
     3961 aaaacagaga tagtaaattt gttatttacc aggtactgta catttttgtt attacagtgc
//
LOCUS       DASWBY010000805         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_70881, whole genome shotgun sequence.
ACCESSION   DASWBY010000805 DASWBY010000000
VERSION     DASWBY010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70881"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2042
                     /gene="metG"
                     /locus_tag="VF870_14585"
     CDS             <1..2042
                     /gene="metG"
                     /locus_tag="VF870_14585"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9253468.1"
                     /translation="ILAKEKILVTSALPYANGPIHLGHLSGAYLPADIYVRYKRLNGD
                     DVIYICGSDEHGVPITISADKEKVSPQVIIDRFHEANKKAFERFGMSFDNYSRTSLPI
                     HHETAKEFFLEFYKRGLFVERKSLQFFDDKANMFLPDRYVEGTCPKCGNEEARSDECE
                     NCGSLYDPSELLNPKSKISGGTPTLKETTHYYFPLGNYQPALEKYVDEMNAKYGWKDN
                     VLQYCRGWFKEGLKDRAITRDLDWGIKVPVDSAAGKVIYVWFEAVLGYISSTKELSQQ
                     KNDPELWKKYWLDPKTKYIAFIGKDNVVFHTIIFPAILMAWNDGGKEQYCLPQNVPAN
                     EFLNFEGKKFSKSRNWGIDVDEFLNLFASDLLRYTLAANLPETRDTDFYWKEFQLRNN
                     SELADILGNFINRTFTFVFKHFDGKVPALGKLEKIDENMIKEISEYPARVADLFEKYK
                     IRDGVNEIMNLARDGNKYFNNSEPWKTVKSNKEQCGTTLNICLQTIYTLSELFSPILP
                     FSTEKLFVMLNTQPVNWKDCGKPQLKEGHQLNQSEILFPKIEDEKIEVQINKLPKSGP
                     EKEKVELITYDDFMKIQLRTAEVIEAEKVSKSEKLLKLKVKLENEERQVVAGIAKSYQ
                     PEDLIGKRVIIVANLQPAKLMGLESKGMILAVETKDGALQVVDIPNSVNNGTRVK"
BASE COUNT          732 a          306 c          408 g          622 t
ORIGIN      
        1 taattttggc taaagaaaaa atactcgtga catctgcact gccttatgca aacggaccta
       61 ttcatcttgg acatttaagc ggcgcatatt taccggcaga tatttatgtt cgatataaaa
      121 gattaaatgg tgatgatgtg atttacattt gtggttctga tgagcacggc gtgcctatta
      181 caataagtgc agataaagaa aaagtttctc cgcaagtaat aatcgaccgt ttccacgaag
      241 caaataaaaa ggcatttgaa agatttggaa tgagtttcga caattattct cgtacaagtc
      301 ttccaattca tcacgaaact gccaaagaat ttttcttaga attctataaa cggggattgt
      361 ttgttgaaag aaaatctctt cagttctttg atgacaaagc gaatatgttt ttgccggatc
      421 gttatgttga aggtacctgt ccaaaatgcg gtaacgaaga agcacgcagt gatgaatgtg
      481 aaaattgcgg atcattgtac gatccatcag aattattaaa tcctaaaagt aaaatttccg
      541 gtggaacgcc aactcttaaa gaaacaacac attattattt ccctcttggt aattaccagc
      601 cagctttaga aaaatatgtt gatgaaatga atgcaaagta tggctggaaa gataatgtgc
      661 ttcagtattg ccgtggttgg tttaaggagg gtttaaaaga tcgtgcgatc actcgtgatt
      721 tggattgggg aataaaagtt ccagtagata gcgccgctgg caaagtaatt tatgtttggt
      781 ttgaagctgt gctaggctac atctcatcta caaaagaatt atcccagcaa aaaaatgatc
      841 cggaattatg gaaaaaatat tggctggatc cgaaaacaaa atacattgca ttcattggta
      901 aagataatgt tgtctttcat acaataattt ttcctgcaat tttaatggca tggaacgatg
      961 gcggtaaaga gcagtactgc ttgccgcaaa atgttcctgc taatgaattt ctgaactttg
     1021 aaggaaaaaa attttccaag agcagaaatt ggggaattga tgttgacgaa tttttaaatt
     1081 tgtttgcttc tgatttatta cgttatacac ttgctgccaa tctacctgaa acccgtgaca
     1141 cggattttta ttggaaagaa tttcagttaa gaaataacag tgagcttgct gatattcttg
     1201 gaaactttat caaccgtaca tttacttttg tgttcaaaca ttttgatggt aaggttcccg
     1261 ctcttggaaa gcttgagaag atagacgaaa atatgattaa agaaatttca gagtatcctg
     1321 cacgagttgc tgatctgttc gagaagtata aaattcgtga tggcgtaaat gaaattatga
     1381 atcttgcacg tgacggcaat aaatatttta acaatagtga accatggaaa actgtaaagt
     1441 caaataaaga acaatgcggt acaactttaa atatttgttt gcagactatt tatactttat
     1501 cggaattatt ctctccgatt cttccttttt caacagaaaa attgtttgtt atgttaaata
     1561 cacagcccgt taattggaaa gactgcggta aacctcaatt aaaagaagga caccaattga
     1621 atcaaagtga aattttattc cccaaaattg aagatgaaaa gatagaagta caaataaata
     1681 agctcccaaa atcaggacct gaaaaagaaa aagttgagtt gattacttac gatgatttta
     1741 tgaaaattca acttcgtacg gcagaggtaa tagaagctga aaaagtttcg aaaagtgaaa
     1801 aacttttaaa actaaaagtg aaattagaaa atgaagaaag acaggttgtt gcaggcattg
     1861 caaaaagtta ccaaccggaa gatttgattg gtaaaagagt tataatagtc gcaaatcttc
     1921 aacctgcaaa actaatggga cttgagtcta agggtatgat tttagctgtt gaaactaagg
     1981 atggtgcttt acaagttgta gatatcccga attcagttaa taatggaaca agagttaaat
     2041 aataatttat atgtagataa atgaaaaa
//
LOCUS       DASWBY010000806         6044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_369534, whole genome shotgun sequence.
ACCESSION   DASWBY010000806 DASWBY010000000
VERSION     DASWBY010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369534"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1972
                     /locus_tag="VF870_14590"
     CDS             <1..1972
                     /locus_tag="VF870_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9253469.1"
                     /translation="GFDQRRISVSINGIPQNDPEDHNVYWLDFPDLLASTELIQVQRG
                     AGSGSVGYPAIGGSINIITSPFSEKPQINLSAAYGSFNTRKYTAAFSSGLINNKYSIY
                     AKLSQIISDGYREKSWSKLNSYHLSAVRYDDKLTSQLNLFGGPISDGLAYTGIAKFAV
                     KDKQLRKDNYSYWEADKNGYTYTLDRRPEEIENFSQPHFELLNEYQINNKMKINSALF
                     LVLGSGFFDYDGSWSVYYDDYFRLKLNGYDSTVVPMNALIRAQVENKQYGWIPKFSLE
                     HANGKLIIGGEFRIHRSDHWGNINYAENIPIGVTKDYQYYFYNGAKNIFGGFIHESYN
                     LTEKINLLGEIQLAYHKYRLYNEKYIGNDFSVDGLFFNPRFGINYRLNENQNVFLSIA
                     RVTREPRLKNYYDAAESSAGEIPQFELNPNGTYNFNSPLVKPETMNDLELGFSHQTQN
                     LTFNLNLYYMLFENEIVKNGKVDRFGQPITGNVDQTTHVGVEMSAIVKIFNSLEIFAN
                     ASYSKNKITTGQYFISENESIDISNNNIGGFPDFLFNIGVQYNSKEVFLKLYTKYVGE
                     FYSDNFDKNLNNYLSDYPAFVAYSDNVNDAYFTMDFYGSYNFILFNGLTESKIFIQVN
                     NIFDKLYSAYAIGKEYFPAAERNFIAGIQVGL"
     gene            1975..2628
                     /locus_tag="VF870_14595"
     CDS             1975..2628
                     /locus_tag="VF870_14595"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEX9253470.1"
                     /translation="MRKCIIIANGKSPTRKVVDYFYARGFETIFCADGGANSAKKLGI
                     IPDFIIGDLDSAAPNTLKYFRSKSTIIKIKRQNDTDVEKCLKFAIKKKFDEVVLLGVT
                     GDRLDHTICNLGIVMKFFSKIKINLVSESSYLIPINKDTIFKSEIGETISIYAFDKNT
                     LITSTGLKYKFYKTALPFGVKESTSNVSVSSSVKLNVSGGIVFVIRDFNFMKKHDLF"
     gene            2615..4012
                     /locus_tag="VF870_14600"
     CDS             2615..4012
                     /locus_tag="VF870_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX9253471.1"
                     /translation="MISFSPLDVIIILLFFLTLLTIGFITSRRTKNDSEDYLLSNRKM
                     GLFLFVLVSVSTWYGGIIGVGEFTYRSGLVSWFTQGFPYYFFAFLFAIFFAKKIRDAS
                     LFTIPDKLGEVYGKQVGLVSAIVVFILVSPAPYLLMTANLISLVFGTNILVSLSIGII
                     LSASYLFKGGFRSNIYADAFQFFVMFAGFIAILVVSIFSFGGIDFLKQNLPASHLSIT
                     GGTSPAFIIVWFFIALWTFADPGFHQRAYAAKSGNVARNGILISIIFFALFDFLTTST
                     GLYAKAVLPDLSNPMMAYPLFAEKILGPGLKGIFYAAMFATIISTLNSFLFLSATTIG
                     RDFIFRLKKDSNENKIKSYTIIGIIISGIISIIIAYLIPSVVEIWYTIGSLFIPGIIL
                     PVISAYYPRLRISSKWILAEIIFAVSISMMWFDFRKSLSGVLNEIEPMLVGLFVAILV
                     HTIGLLRKSVSLDKR"
     gene            complement(3977..4903)
                     /locus_tag="VF870_14605"
     CDS             complement(3977..4903)
                     /locus_tag="VF870_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX9253472.1"
                     /translation="MITEYSFWFIFSAVIAVMLFIDLYVTDHRRGKITLKASLIWSGV
                     WIITALLFNLFLYIYLEDGHTKALEFITGYIIEKSLSVDNLFVFLMIFSVMNIKPENQ
                     PHILKWGILSAIVFRIAFIIAGVTLIKLFQPIIYVFAIILLFAAYKMAFGSEQKIDVE
                     NNWLIKIARKYFKLNTSYKGKKFFIKVDKKVYITTTFLTLLLIESSDIVFAVDSIPAI
                     IAITKDTFIIISSNIFAILGLRALYFALAGLVDLFVYLKYGVAIILFYVGIKMLISEF
                     YKIPTEVSLFIILLFLGGSIILSLIKRNRFSQ"
     gene            complement(4919..5464)
                     /locus_tag="VF870_14610"
     CDS             complement(4919..5464)
                     /locus_tag="VF870_14610"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEX9253473.1"
                     /translation="MYLPTKIFFTKGVGRHKEYLSSFELALRNARIEICNLVTVSSIF
                     PIGCKRVSIDEGVKLITPGQITHCVMARNSTNEPNRLIAASIGVAIPADTNAYGYLSE
                     HHPYGQPEKICGEYAEDLAATMLASTLGVEFDPEKDWSEREDIYKMSGKIVRTFNVTQ
                     SAEGDKNGLWTTVIAVGVFLP"
     gene            complement(5488..>6044)
                     /gene="tyrS"
                     /locus_tag="VF870_14615"
     CDS             complement(5488..>6044)
                     /gene="tyrS"
                     /locus_tag="VF870_14615"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX9253474.1"
                     /translation="TMPLLVGTDGVEKMSKSYDNYIGISDEPSQIFGRTLSIPDSLIY
                     QYFELATNVSNKKLTEIKSQLSDGTTNPRDIKRELARTLVTMYHNQEAALTAQEEFDN
                     IFINKGLPDEIEEFNVGDKREINILDLIILVNFAPSKGEARRLLTQGGVSIDGEKIND
                     VQSSISVKSGMILKVGKRKFIKLI"
BASE COUNT         1988 a          950 c         1016 g         2090 t
ORIGIN      
        1 cggatttgac cagcgaagaa tatcggtttc tattaatggc attccacaaa atgatcctga
       61 agaccataac gtttattggt tggattttcc tgatttactt gccagcacag aacttattca
      121 ggtacaacgg ggcgcaggtt ctggcagcgt tggatatccg gcaatcggcg gatcgattaa
      181 tattattaca tctccatttt cagaaaaacc acagattaac ctttcggctg cttacggaag
      241 ttttaatacg agaaaatata cggcagcttt ttctagcgga cttataaaca ataagtactc
      301 tatctatgca aagctttctc aaattattag tgatgggtac agggaaaaat cctggtcaaa
      361 actaaattca tatcatcttt ctgctgtgcg atatgatgat aagttaacgt cgcaattaaa
      421 tctttttggc ggaccaattt ctgacggtct tgcatatacc ggaatagcca agtttgctgt
      481 aaaggataaa caattaagaa aagataatta ctcctattgg gaagcagata aaaatggtta
      541 cacttataca ttggatagaa gaccagaaga aattgagaac ttttctcaac cacattttga
      601 gttgttgaat gagtatcaga taaataataa gatgaaaatc aattcagctt tattcttagt
      661 tcttggttcc ggtttttttg attatgatgg aagctggtca gtttattatg atgattattt
      721 cagattgaaa ttaaatggtt atgattcaac agtagttcca atgaatgctt tgatcagagc
      781 tcaagttgaa aacaaacagt acggatggat tccaaagttc agtttagaac acgccaatgg
      841 taagttgatt attggcggag agttcagaat ccatagatct gaccattggg gaaatattaa
      901 ttatgctgaa aatattccta tcggtgttac aaaagattat cagtattatt tctacaatgg
      961 cgcaaaaaat atttttggtg gatttataca cgaatcatat aatcttacag aaaagataaa
     1021 ccttcttggt gaaattcaat tggcttatca taaatacaga ttatacaatg aaaaatatat
     1081 aggaaatgat ttttctgttg atggattatt ttttaatccg cgatttggaa ttaattatcg
     1141 attaaatgaa aatcaaaatg tattcttatc tatcgcaagg gttacgcggg aaccccggtt
     1201 aaaaaattat tacgatgcag cagaatccag cgcaggagag attccgcaat ttgaattaaa
     1261 cccaaatggt acttataatt ttaattctcc attagttaag ccggaaacga tgaacgattt
     1321 agaactgggc ttctcacatc agactcaaaa tcttacattt aatcttaatt tatactatat
     1381 gctttttgag aatgaaatag ttaagaatgg taaagtcgat cgttttgggc agccaattac
     1441 aggaaatgtt gatcaaacaa ctcacgttgg tgttgagatg agtgcaatag ttaaaatatt
     1501 taatagctta gaaatatttg caaatgcaag ctacagtaaa aataaaatta ctaccggaca
     1561 gtattttatt tctgaaaatg aatcaataga tatttccaat aacaatatag gcggatttcc
     1621 ggactttctt ttcaatatcg gagttcagta taattctaaa gaagtatttc ttaaacttta
     1681 cactaaatat gtcggtgagt tttattctga taactttgat aaaaatttaa ataattattt
     1741 gagcgattat cctgcctttg ttgcttattc ggataatgta aatgatgcct attttactat
     1801 ggatttttat ggaagctaca acttcatttt atttaatggt ttgactgaat caaaaatatt
     1861 tatacaggtt aataatatat ttgataaatt atattcggct tatgcaattg gtaaagaata
     1921 ttttcctgct gcagaaagaa attttattgc cggaattcaa gtgggattat agttatgaga
     1981 aaatgtataa taatagcaaa tggtaaatca ccaaccagaa aagttgttga ttatttttat
     2041 gccaggggat ttgaaacaat attttgtgca gatggaggtg ctaattctgc taagaaactt
     2101 ggcattattc cagattttat tattggtgat ctggacagcg ctgcaccaaa tactctaaaa
     2161 tattttcgat ccaaatccac gataatcaaa ataaaaagac agaatgatac tgatgttgag
     2221 aagtgcttga agtttgcaat caagaaaaag tttgatgaag ttgttttact tggagttacg
     2281 ggtgacagat tagatcacac aatttgtaac ctgggaatcg ttatgaagtt ctttagtaag
     2341 attaaaataa atctcgtttc tgaaagttct tatcttatcc cgataaataa ggatacgatt
     2401 ttcaaatctg aaattggtga aacaatatca atttatgctt ttgataaaaa tactttgata
     2461 acttcaacag gattaaagta taagttttac aaaaccgctt taccatttgg tgtaaaggaa
     2521 agcacttcaa atgtttcagt ttcaagttca gtcaaattga atgttagcgg cggaattgtt
     2581 tttgtcattc gtgattttaa ttttatgaaa aaacatgatc tcttttagtc ctcttgatgt
     2641 aataattatt ctgcttttct ttttgactct cctcacgatt ggctttataa catccagaag
     2701 aacaaaaaac gattctgagg attatctttt atccaacaga aaaatgggat tgtttctttt
     2761 tgtattggtt agtgtttcta cttggtatgg tggaattatt ggagtagggg agtttactta
     2821 cagatctggt ttggtcagtt ggtttacgca aggatttcca tattactttt ttgcttttct
     2881 ctttgcaatt ttctttgcaa aaaaaatccg tgatgcttca ttatttacaa ttccggataa
     2941 acttggtgaa gtttatggaa aacaagttgg attagtatct gcaatagttg tatttatttt
     3001 ggtatcgcct gctccatatt tattaatgac agcaaatctt atttctttgg tctttggcac
     3061 aaacatttta gtatcgctga gtatcggcat tattctatca gcttcgtatt tatttaaagg
     3121 cgggtttcgt tcaaacattt atgcagatgc atttcagttt ttcgtgatgt ttgccggatt
     3181 catcgccatt cttgttgtaa gcattttttc atttggagga attgattttc tgaaacaaaa
     3241 tttacccgcc tcacatttat caattaccgg tggaacttct ccagctttta ttattgtatg
     3301 gttctttatt gcattatgga cttttgctga tcccggattt catcaacgtg catatgctgc
     3361 aaaatctggg aatgtggcaa ggaatgggat cctaatttcc ataatctttt ttgctttgtt
     3421 tgattttcta acaacttcaa caggactcta tgctaaagca gttttacctg atttatcaaa
     3481 tcctatgatg gcctatccat tatttgcaga aaaaatatta ggaccgggat taaaaggaat
     3541 attttacgct gctatgtttg cgacaataat ttcaactctt aacagctttt tatttttaag
     3601 tgctaccaca attggaagag attttatttt tagattaaag aaagattcaa atgaaaataa
     3661 gataaaatct tacaccatta tcggaataat tatttcaggt ataatttcaa ttattattgc
     3721 ttatttaatc ccttcagtag ttgagatctg gtacacgatc ggttcactat ttattccggg
     3781 aattatttta cctgtaataa gtgcttatta tccccgacta agaatatctt caaaatggat
     3841 attagcagaa attatttttg cagtaagcat atctatgatg tggtttgatt ttagaaaatc
     3901 tttaagtgga gtattaaatg aaattgaacc aatgctagtc ggattgtttg tagcaatttt
     3961 ggtccacaca attggactat tgagaaaatc tgtttctctt gataagagat aagataattg
     4021 atccaccaag aaatagaaga ataataaaaa gtgatacttc agtagggatt ttataaaact
     4081 ctgaaattaa cattttaatt cccacataaa ataaaataat tgccacacca tactttaagt
     4141 aaacaaacaa atcaaccaat ccggccaaag caaaatacag agcacgaaga ccaagtattg
     4201 caaaaatatt tgatgaaata attataaagg tatcctttgt aatcgcaatt atggcgggaa
     4261 ttgaatcaac tgcaaaaaca atatctgaag attctataag cagcaatgtt agaaacgtag
     4321 ttgtgatata aactttcttg tcaactttta taaaaaactt tttgccttta taagaagtgt
     4381 ttaacttaaa atattttctt gcgattttta tcagccagtt attctcaaca tcaatttttt
     4441 gttcactgcc aaacgccatt ttataggcgg cgaacagaag tataattgca aatacataaa
     4501 taattggttg aaaaagtttg atgagtgtaa cgccggcaat gataaaagct attctaaaaa
     4561 caatagcact taaaattccc cacttaagaa tatgcggttg attttcaggt ttaatattca
     4621 taacactgaa tatcattagg aacacaaaaa gattatctac tgaaagtgat ttttctataa
     4681 tgtagccggt tatgaattca agtgctttgg tatgtccgtc ttcaagataa atgtacaaga
     4741 aaagattaaa aagaagagcg gtgataatcc atacaccgct ccaaataaga cttgctttta
     4801 aagtgatctt tccgcgtcta tgatccgtaa catataaatc aataaataac attaccgcaa
     4861 taaccgcgga aaatataaac cagaaagaat attcagttat caatcaatcc ttcttttgtt
     4921 aaggaagaaa aacaccaaca gctataactg ttgtccacaa tccgttttta tcaccttctg
     4981 ctgattgagt aacattgaaa gtacgaacaa tttttcccga catcttataa atgtcttctc
     5041 tttcgctcca atctttttcc ggatcaaatt caacacccag agttgaagca agcattgttg
     5101 cagccagatc ttcagcatat tcaccacaaa ttttttcagg ctgtccgtat ggatgatgtt
     5161 cagataaata accgtaagca ttcgtgtctg cgggtattgc tacaccaata gaagcagcaa
     5221 tcagtctgtt tggttcattt gttgagttac gtgccattac acaatgcgta atttgtcccg
     5281 gagtaataag ttttacacct tcatcaatcg aaacacgttt acaaccgatt ggaaaaatac
     5341 tgctgacagt tacaagattg cagatttcga ttcttgcatt tcttaatgca agctcaaaag
     5401 agcttaaata ctctttgtgt cttccaacac ctttggtgaa aaatattttt gttggtagat
     5461 acaattgcgt ccctttctga tactaagtta aatgagttta atgaatttac gtttgccaac
     5521 tttaagaatc attcctgact taacagaaat actactttga acatcgttta ttttttctcc
     5581 atcaattgaa actccgcctt gtgttaacaa tctgcgtgct tctcctttgg atggagcgaa
     5641 atttactaaa ataattaaat ctaatatatt gatttctctt ttatccccaa cattaaattc
     5701 ttcaatttca tctggtaaac ctttgttgat gaaaatgtta tcaaattcct cctgagcagt
     5761 taaagctgct tcttgattat gatacattgt aactaatgtt cttgcaagtt ctcgttttat
     5821 atctcttgga ttagttgtac catctgataa ctgagatttt atttcagtaa gttttttgtt
     5881 cgatacattt gttgcaagtt caaaatattg atagataaga ctatctggaa ttgacaaagt
     5941 ccttccaaaa atctgagaag gttcatcaga tataccaata tagttatcat aagatttact
     6001 catcttttca acaccatcag ttccaactaa caatggcatg gtta
//
LOCUS       DASWBY010000807         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_108550, whole genome shotgun sequence.
ACCESSION   DASWBY010000807 DASWBY010000000
VERSION     DASWBY010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108550"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VF870_14620"
     CDS             complement(<1..627)
                     /locus_tag="VF870_14620"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9253475.1"
                     /translation="MIKRFTILLILINSFFIYSQNTGNLSGIVKDSKTGEALPGVNVI
                     IKGTYYGAATDINGKFLINNISVGSYNVDISLIGYKTVQYTGIQIESNKTKELTVKLE
                     ETVLTLDQDVVVIGEKPLMDVEETQSKKIISKEEIDIAVLENIADIVTQQAGVVKSDN
                     EIHIRGGRSYENAFLLDGVSVQDPLAGTGFGLQLSANAIEEVEVITGGF"
     gene            complement(624..>1596)
                     /locus_tag="VF870_14625"
     CDS             complement(624..>1596)
                     /locus_tag="VF870_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253476.1"
                     /translation="FGWNVDDLDGTSTILKINIALNDTTNANNIVSLDGSVRTITLRT
                     KDFTSANPLMDILIEGLESNIFSEKLPGLKMDDYNTFYVQAVDVAGASSKFIALPDTG
                     KTWFVKKPKGDIVIIDDYSTTDGAAAFYNSMMDSLGLSGKYDIYDYLNQKPPYTSVTF
                     LQTIKLFKYVLWYSDNNPSLELASATVQKYIDAGGKIAFSMQFPQNVDPYLLQGFLPI
                     STDTVYSKTTLLPNTIISADTTDFTYPKLTTITSLFRIKSFYLNPNGAIPIYYFPNNE
                     LSGFIGFFNNSKSLFFYGLPLSKSNGGAANVKSLLSKIFFEDFGVVP"
BASE COUNT          533 a          291 c          235 g          537 t
ORIGIN      
        1 gaatccaccg gtaataactt caacctcttc aattgcattt gctgagagct gtaaaccaaa
       61 ccctgtgcct gcaagcggat cctgaactga aactccatct aacaaaaaag cattttcata
      121 agatctgccg ccgcgaatgt gaatttcatt atcagatttt acaacacctg cctgctgggt
      181 aactatatct gcaatatttt ctaatacagc aatatcaatt tcttcttttg aaattatctt
      241 tttactttgt gtttcttcaa catccatcag gggtttctca ccaataacaa caacatcctg
      301 atcaagtgta agaacagttt cctcaagctt tacagtaagc tctttagttt tgtttgattc
      361 gatttgtatt cctgtgtatt gaactgtctt atatccaatt aaagaaatgt caacattata
      421 acttccgacg gaaatgttat taatcaagaa cttaccgttt atatctgttg ctgcaccgta
      481 atatgttcct ttaataataa catttacacc cggtaaagct tctccggttt ttgaatcttt
      541 tacaatacca gaaagattac ctgtgttttg agaatagata aagaatgaat taataagtat
      601 cagcagaata gtaaatcttt ttatcatggc acaactccga aatcttcaaa aaagatttta
      661 ctaagcagac ttttaacatt tgctgcccct ccattggatt tacttagtgg cagaccataa
      721 aagaataacg attttgaatt attaaaaaat ccaataaacc cactaagctc attattcggg
      781 aaataataaa taggtattgc accgttagga tttaaataaa acgatttaat cctgaataaa
      841 ctggttatag ttgttaattt aggataggta aagtccgttg tatcggctga gataatagta
      901 tttggaagca gtgttgtttt agaataaaca gtatccgtac tgattggcaa aaatccctgc
      961 agcaaataag gatctacatt ctgagggaac tgcattgaaa aggcaatctt tccaccagca
     1021 tcaatatatt tttgaactgt tgcagatgct aattccaatg aaggattatt atcactatac
     1081 caaagaacat atttaaacaa tttaattgtt tgtaaaaaag ttacgcttgt atatggcggc
     1141 ttttgattta aataatcata aatatcatat ttaccactta accctagact atccatcata
     1201 gaattataaa atgcggctgc gccatcagta gttgaataat catctattat aacaatatca
     1261 cctttaggtt tttttacaaa ccaggttttt cctgtatccg gtagtgcaat aaattttgaa
     1321 gatgccccag ccacatcaac cgcctggaca taaaaggtat tataatcgtc catctttaat
     1381 ccgggtaatt tttcagaaaa gatatttgat tcaagtcctt caattaaaat atccattaat
     1441 ggatttgctg atgtaaaatc ttttgttctt aaagttattg tcctaacact tccatccaaa
     1501 gaaacaatat tatttgcatt tgtggtatcg ttaagtgcaa tattaatttt aagtatagta
     1561 cttgttccat caaggtcatc aacattccaa ccaaaa
//
LOCUS       DASWBY010000808         6421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_401574, whole genome shotgun sequence.
ACCESSION   DASWBY010000808 DASWBY010000000
VERSION     DASWBY010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401574"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VF870_14630"
     CDS             <1..531
                     /locus_tag="VF870_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEX9253477.1"
                     /translation="RIDRYLISNGGGLLRLTNTNFSVSLSLAGDKLTSSSKKTNIQQD
                     EYLQASETSIYQGLYSNKDADFSIPWDISLNYYYNLSRPIPTKETTTSSISGSLNFNL
                     TPKWKFSATGSYDLQRKEFAAPQIRISRDLHCWVMNFTWNPIGTYRGYNFEIRVKAPQ
                     LQDLKITKRDQFYEGR"
     gene            534..992
                     /locus_tag="VF870_14635"
     CDS             534..992
                     /locus_tag="VF870_14635"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9253478.1"
                     /translation="MLNYIIDGNNLIGKISSLINLQKKDKQASREKLVYILDRYFIQK
                     KANVTLHLDGHPGNKINSSKMKIIYSENLTADEKIKKQVSQSKSPRNIVVITSDSNLA
                     QFAKVCAATVISSDEFSAEIKKSSDQVDEQSKIDSINNIEEFKKLFGVDD"
     gene            1093..3360
                     /locus_tag="VF870_14640"
     CDS             1093..3360
                     /locus_tag="VF870_14640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253479.1"
                     /translation="MHNSTFFIRNIFGVLVFFVISSNLFAQIDNPLTDHVPIEYIKHI
                     QGYQSDASDAVITDENGYDNFNLGNAFAEPHLVQNPNNPLQYFTSFNTNTAYRTNDGF
                     NWLISAPPFGASTYGDPVNAYDSLGNLFYENMSGSGTIQNSRVIRSTDNGTTWGASVI
                     AVLGNDKNWIAADQTSGPFANYLYTTMTNSGTGSFYRSTDNGATFSNTNTFTTQTLPG
                     MMVAVGPNTAAGDVPGGCVYVVTNSGSSFASTYTFYVSTDGGATFTLKSTQFFSGYVG
                     TNVGGRNSVENMRTRPYPFITADNSYGPNRGRLYLIYASNTPAGDFNKPDIFCRYSTD
                     QGATWSLPVVVNDDESTTANHQWHPSIWCDKTSGRLFVKWMDTRDTPTSDSAHIYASY
                     SDDGGLTFAPNQKITTAKMKINCSTCGGGGTPRYQGDYDAITAIDNQSLMVWTDFRTG
                     SFGSYVGYFPDFAMLVSPAIDTLVNDRDSLFYEINVPSVKLYDTDAIFSATITPTPAS
                     GSIEIDFPDGNTLSTFPGTVSMKVRTTSNVSVGTYTIVVTGKGPNGTPLHKRNVTLVV
                     GMAIPVELTAFNASINKNDVILNWTTATELNNLGFEIQRRNEGGSFESVGFIGGKGTT
                     TQTNNYSFVDNSVDAGIYTYRLMQKDFDGSFAYSQEVEVEINLPLEYSLDQNYPNPFN
                     PTTTIRYAIPQDNFVSIKLYDVLGNEVITLVNEQRQAGRYEMLFNAADIASGVYYYQI
                     KAGDFIRTKKLLLMK"
     gene            3530..5536
                     /locus_tag="VF870_14645"
     CDS             3530..5536
                     /locus_tag="VF870_14645"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9253480.1"
                     /translation="MKKFFSVLFILLLTSITFSQILDEQYFKDNHEVYFKFTLPDLTE
                     LNTLTRIISIDNLEGNTVYAYANETEFYEFQKLKYPYTLLTHPGKLIEPEMSSNIDGI
                     TAWDIYPIYDAYIAMMFQFQSNYPNICRIVDAGNTVQGRKILFAVISDNVNQRETEPQ
                     FMYTSSIHGDELTGYVLMLRLIDSLLTTYGTNSRITNIVNSAEIWINPLANPDGTYRS
                     GNSTVYGATRGNYNNYDLNRNFPDPIYGVNVNQQIETARFRAVAETNNFALVANFHGG
                     TEVVNYPWDSWTNEYPDYKHHPDETWYQYISHLYADTCQANAPSNYMNGYDDGTTNGG
                     AWYIITGGRQDYTNYYRWGREVTIEISDTKLIPAAQLPAHWNYNRKSFINYMESVIKG
                     VNGIITDTVGNPVKASVTVLNHDLDNSEVYSDSTGFYLRMLAPGNYSFRFEAPDYYPK
                     TINNVAVGSYNSLTILNVELVSSIIPVELISFTSSVNGNNVSLNWMTATETNNMGFNL
                     ERTQISSIQSPIGKDDWKSISFIHGNGTTTEPQSYFYQDENLVSGNYRYRLKQIDFDG
                     SFGYSNIVEIEIGSPDNFMLEQNFPNPFNPSTIIKYSLPINSNVILKVYDVLGKEVAI
                     LVDEEKSAGDYEAEYKAENLSSGIYFYELKIGDVILTKKMLLLQ"
     gene            5636..>6421
                     /locus_tag="VF870_14650"
     CDS             5636..>6421
                     /locus_tag="VF870_14650"
                     /inference="COORDINATES: protein motif:HMM:NF041895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HEX9253481.1"
                     /translation="MKNYIILIIIFISFITFAYSGLFEYDLLGGTQLNGSGCVCHTLE
                     IDTSVSVWIEGPDTLKTGETGLYRMYLANGPAEAGGYNVAGRFGTMVLVDSFSYWNYL
                     SPNELTQAFPLVFPTPQDTIYWEFGYTAPDSILADTLYSCGLSIVFDTIPDFRDRWNF
                     GPKFPVTIIENIVPVELISFSALQSGSDIKLSWFTATETNNLGFEIERAVNLTEGRNL
                     SWTVIGSIEGNGTTAGKSSYSFVDKNPNQGKSYYRLKQIDFNGS"
BASE COUNT         2121 a         1065 c         1168 g         2067 t
ORIGIN      
        1 aggatcgata gatatttaat aagcaatggc gggggattat taagattgac aaatactaat
       61 ttttctgttt ccttatcgct tgctggagat aaattgactt catcaagcaa gaaaacaaac
      121 atacaacagg atgaatattt acaggcatca gaaacaagta tttatcaggg attgtacagt
      181 aataaggatg ctgatttttc tataccctgg gatatttcac ttaattacta ttataattta
      241 tcgcgaccca ttcctacgaa agaaactact acatcaagta ttagcggtag tttaaacttt
      301 aatcttacac ccaaatggaa attttcagca actggcagct atgatttaca acgaaaagaa
      361 tttgctgcac ctcaaattag aatctcacgt gatcttcatt gctgggttat gaacttcacc
      421 tggaatccta ttgggacata tcgcggttac aattttgaaa tacgagttaa ggcaccgcag
      481 ctgcaggatt taaaaattac taaacgcgat caattctacg aaggaagata gagttgctta
      541 attatattat tgatggaaat aatcttattg gtaaaatatc ttctttgata aatcttcaaa
      601 aaaaagataa gcaggcttct cgcgaaaaac ttgtttacat acttgatcgt tattttatac
      661 agaagaaggc taatgttact ctgcatttag atgggcatcc gggtaataaa ataaactcat
      721 caaagatgaa aataatatat tcagaaaatt taactgctga tgaaaagatt aagaagcagg
      781 tatcacaatc caaatctcca agaaatattg tagttataac ttcagattcc aaccttgcac
      841 aatttgccaa agtttgtgca gcaactgtca tttccagcga tgaattttcg gctgaaatta
      901 aaaaaagctc cgatcaggtt gatgaacaat ctaaaattga ttctataaat aatattgaag
      961 agttcaaaaa gctttttgga gttgatgact aataaaaata atattcttca ttgatattta
     1021 tttctaaata tttcctatta tgcaactgcc tttttaaata tttaacttat taatatcctt
     1081 ggaggttcat tgatgcataa ttctacattt tttattagaa atatttttgg ggttttagtt
     1141 ttctttgtca tttcatcaaa tcttttcgca cagattgata atcccttaac tgatcacgtt
     1201 ccaatagaat atatcaaaca tattcaaggt tatcagagcg atgcttcaga tgcagtgata
     1261 acagatgaga atggttatga taattttaat ttaggtaatg ctttcgcgga gccgcatctt
     1321 gttcaaaatc ctaacaaccc attacaatat tttacttcct tcaacacaaa tacggcatat
     1381 agaacaaatg atggatttaa ttggttaata agtgccccac catttggtgc ctctacatat
     1441 ggagatcctg taaatgctta tgacagttta ggaaatctat tttatgaaaa tatgtcaggc
     1501 tctggaacaa ttcaaaattc aagagttatc agatcaaccg ataacggtac tacctgggga
     1561 gcttctgtaa tagcagtatt aggtaatgat aagaactgga ttgctgcaga tcaaacctca
     1621 ggaccttttg ctaattattt gtacactact atgacgaata gcggtactgg aagcttttat
     1681 cgtagtacag ataatggagc gactttttcg aacacaaata catttacaac gcaaacactt
     1741 cctggaatga tggttgcagt cgggccaaac actgcagctg gcgatgtacc tggtggttgt
     1801 gtttacgttg ttacaaattc aggaagttct tttgcatcaa cttatacgtt ttatgtttct
     1861 actgatggtg gtgcgacttt tacattaaaa tcaacacaat ttttttccgg gtatgttggt
     1921 acaaatgttg gcggcagaaa ttcagtggaa aatatgagaa ccaggccata tccttttatt
     1981 acagcagata acagttatgg cccaaataga ggaagactat atttaattta tgcttccaac
     2041 actcccgctg gtgattttaa taaaccggac attttctgta gatattctac tgatcaaggt
     2101 gcaacatggt cattacccgt agtagttaat gatgatgaaa gcacaacggc taatcatcaa
     2161 tggcaccctt ccatttggtg tgataaaacc tccggccgct tatttgtaaa atggatggac
     2221 acccgtgata ccccaacaag cgatagcgca catatttatg caagttattc tgatgatggc
     2281 ggattaactt ttgctccgaa tcaaaaaatc acaactgcta aaatgaaaat taattgttca
     2341 acctgcggcg gaggcggcac tccaagatat caaggtgatt atgatgctat aactgcaatt
     2401 gataaccaat cacttatggt gtggactgat ttcagaacag gatcatttgg aagttatgta
     2461 ggttattttc cggattttgc tatgctcgtt tcgcctgcaa tcgatacctt ggtgaatgat
     2521 cgggattcgc ttttttatga aattaatgta cccagtgtaa aactgtatga tactgatgca
     2581 atcttctcag caacgattac accaacacct gcatcgggat cgattgaaat tgatttcccc
     2641 gatggcaaca cactatctac ttttccggga acagtttcaa tgaaggttag aactacgagt
     2701 aatgtttccg ttggtaccta tactattgta gttacaggta agggtccaaa cggtacacct
     2761 ttacacaaac gtaatgtaac tttagttgtt ggaatggcca taccagttga attgacagca
     2821 tttaatgcat caattaataa aaatgatgta atcctcaatt ggacgacagc aactgagtta
     2881 aataatcttg gatttgaaat tcaaagaaga aatgaaggtg gatcttttga atccgttgga
     2941 tttattggcg gaaaaggtac gacaacacaa acaaataatt attcttttgt tgataactct
     3001 gttgatgccg gaatctatac ttacagatta atgcagaaag attttgatgg atcatttgca
     3061 tactctcagg aagttgaagt agagatcaat ctgcctcttg aatactcact agatcagaac
     3121 tatccaaacc cgttcaaccc aactacaaca ataaggtatg caattccaca ggataatttt
     3181 gtaagtatta aattatatga tgtcctgggt aatgaggtaa taacattggt taatgaacaa
     3241 agacaagccg gcagatatga gatgttattt aatgcagcag atattgcaag cggagtctat
     3301 tattaccaga ttaaagctgg agattttatt cgaactaaaa aattacttct gatgaaataa
     3361 ttggcaggtc ctcttttaga gaggaattat gcaggatatt ttaaaggcac ggtaacccgt
     3421 gccttttttt gttgccggat gatagcttta ttgatttcaa atcttcttaa ttttaacata
     3481 ataaaaatat taattttgct taatcacatt taaccaagca gagcctctta tgaaaaaatt
     3541 cttttctgtt ttattcatat tacttcttac atcaataaca ttttcgcaaa ttttagatga
     3601 gcagtatttt aaagataacc atgaggttta ttttaaattt actttacccg atctgacaga
     3661 attaaatact ttaacacgta taatctcaat tgataatctt gagggtaata ccgtttatgc
     3721 gtacgcaaat gaaactgaat tttatgaatt tcaaaagcta aaatatcctt acaccttgct
     3781 gactcatccc ggaaaattaa ttgaacctga aatgtcatca aatattgatg gaattactgc
     3841 gtgggatatt tatccaattt atgatgctta catagcaatg atgttccagt ttcaaagcaa
     3901 ctaccctaat atttgcagaa ttgtagatgc tggtaataca gtgcaaggaa gaaaaatttt
     3961 atttgcagta atttctgaca atgtaaatca aagggaaact gaaccacagt ttatgtacac
     4021 ttcttcaatt cacggagatg agctaaccgg atatgttctt atgttaaggc ttatcgattc
     4081 attactaaca acttatggaa caaattcaag aattacgaat attgttaaca gtgctgaaat
     4141 atggattaat ccattagcaa atccggacgg cacttacaga agtggcaata gcacagttta
     4201 tggcgcaaca aggggcaatt acaataatta tgatcttaac agaaactttc ctgatccaat
     4261 atatggtgtt aatgtaaatc agcagattga aacagcaaga tttcgagcag ttgctgaaac
     4321 aaataacttt gcccttgttg caaattttca cgggggaact gaggttgtaa attatccgtg
     4381 ggattcctgg acaaatgaat atccggatta caaacatcat ccagatgaaa catggtatca
     4441 atatatttca catttatatg ctgatacatg tcaggctaat gctccatcaa attatatgaa
     4501 tggttatgat gatggaacta cgaacggtgg ggcctggtat attataaccg gaggaagaca
     4561 agattataca aattattaca gatggggcag ggaagtaaca atcgaaattt cggatacaaa
     4621 actcattccc gcagcgcagc ttcctgctca ctggaattat aacagaaaat catttattaa
     4681 ttatatggaa tctgttataa aaggtgttaa tggaattata acagatactg ttggtaatcc
     4741 ggttaaagca agtgtaacgg tcctgaatca tgatttagat aattctgaag tatattctga
     4801 ttcaacagga ttttatttgc gtatgcttgc gcccggcaat tattcattca ggtttgaggc
     4861 tcctgattat taccctaaga ccataaataa tgttgctgtc ggttcttaca attcattaac
     4921 aatcttaaat gttgaacttg tttcttcgat aattcctgtt gaattaattt catttacttc
     4981 ttcagtgaat ggaaataatg tttcgttaaa ttggatgaca gcgactgaaa caaataatat
     5041 gggatttaat ttagaaagaa cacaaatcag cagtattcag tctccaattg gcaaagatga
     5101 ttggaagtca atcagtttta ttcatggaaa cggaactaca acggagccgc aatcttattt
     5161 ctatcaagat gaaaatttag tttcaggaaa ctaccgttat agattgaagc agattgattt
     5221 tgatggttct tttggatatt caaatattgt agaaattgag attggttctc cagataattt
     5281 tatgttagaa cagaactttc caaacccttt taacccttca acaataatta aatatagttt
     5341 accaattaat agtaatgtta ttcttaaagt ctatgatgtt cttggtaaag aagttgcaat
     5401 tttagtggat gaagaaaaat ctgccggtga ttatgaagca gaatataaag cagaaaacct
     5461 ttccagcgga atctactttt atgaattaaa aatcggcgat gttatcctga caaaaaagat
     5521 gttattactg caataaaata ttttgcggga tgctaaggct tcccgcatcc catcttttga
     5581 ataatctctt aattttatat cttacctaag aatatccgat aaataaatat tactaatgaa
     5641 aaactatatt attcttatta taatcttcat ttcctttatc acttttgcat actccggatt
     5701 gtttgagtac gatttacttg gtgggacgca gcttaatgga agtggatgtg tatgccatac
     5761 tctagaaata gatacaagcg tttctgtctg gattgaggga ccggatacat taaagactgg
     5821 tgagactggg ctgtatagga tgtaccttgc aaatggtcca gctgaagcag gcgggtataa
     5881 tgttgctggt cgatttggta caatggtatt agtagattca ttttcttatt ggaattatct
     5941 ttcaccaaat gagttaacac aagcttttcc cctggtattt cctactccgc aggatacaat
     6001 atactgggag tttggttata cagccccgga ttcaatttta gctgatactc tttattcctg
     6061 cggattgagt atagtctttg atactattcc tgattttaga gacagatgga actttgggcc
     6121 taagtttcca gttacaataa tagaaaacat tgtccctgta gaattaattt ctttttcagc
     6181 gttacagtca ggaagtgata ttaagttaag ttggtttacc gcaactgaaa caaataatct
     6241 gggttttgag attgaacgcg ccgtaaattt aaccgaaggg cgaaatcttt cctggacagt
     6301 tataggatct atagagggga acggaacaac agcaggcaaa tcatcttatt cttttgttga
     6361 taaaaatcca aaccagggca aatcttacta cagattaaaa caaatagatt ttaatggttc
     6421 a
//
LOCUS       DASWBY010000809         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_260905, whole genome shotgun sequence.
ACCESSION   DASWBY010000809 DASWBY010000000
VERSION     DASWBY010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260905"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF870_14655"
     CDS             <1..204
                     /locus_tag="VF870_14655"
                     /inference="COORDINATES: protein motif:HMM:NF017765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HEX9253482.1"
                     /translation="VTTDIGNRFILAILSASASYIAVPAAMRIAAPEADPGLYIPMAL
                     GITFPVNITIGIPLYFTIVNYFA"
     gene            204..1358
                     /locus_tag="VF870_14660"
     CDS             204..1358
                     /locus_tag="VF870_14660"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX9253483.1"
                     /translation="MEKLVTSEIKLGIIAGGQLGKMLVLAASNWNVKSYVLDKDHHCP
                     ASSSCTFFFRGDQLNYDDVYRFGQMVDVLALELENVNKEALKKLKQEGKKIYPDPDKL
                     EIIQDKGLQKQFYQKIKVPSSPFSLFNSKQEILSAVDSGKLQLPFVQKLRKGGYDGRG
                     VSIIRSKEDLINLLDGASVIEHLVDIEKEISAIVARNERGEVKCHPTVEMEFNPNANL
                     VEKLICPSTLSKETEEEAEKIAVKIITELDLYGILAVEMFVDKNKNIWVNEVAPRPHN
                     SGHHTIESAITSQFEQLLRAIFNLPLGSTKLKMPAVMINLLGESGYEGKVRYEGLTEC
                     MAVEGVKIHLYGKKITKPFRKMGHVTVLAPTIDEAKKKADFVKEKMKVIS"
     gene            1355..1855
                     /gene="purE"
                     /locus_tag="VF870_14665"
     CDS             1355..1855
                     /gene="purE"
                     /locus_tag="VF870_14665"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX9253484.1"
                     /translation="MKNILVSIIMGSDSDLEVMKEAGKVLKEFDVSFEITVVSAHRTP
                     KRLVEFSENAHKRGVQVIIAGAGGAAHLPGMVAAISPLPVIGVPVKSSNSLDGWDSIL
                     SILQMPGGVPVATVALNNAKNAGLLAAQMLSVADENLRNKVISYKEKLNSDVLKKIDK
                     VEKSFE"
BASE COUNT          681 a          339 c          428 g          622 t
ORIGIN      
        1 gttactacgg atattggtaa cagatttatt cttgcaattc tttctgcaag tgcatcttac
       61 attgcagttc ccgctgcgat gaggattgcg gccccagaag ctgatcccgg gctttatatt
      121 ccgatggctt tgggaattac ttttcccgtt aacattacaa taggtatccc tctttatttt
      181 acaatcgtaa actattttgc ataatggaaa aattagtaac atcagaaatt aaacttggta
      241 taatcgccgg aggccagctt ggtaaaatgc tcgtgctcgc ggcaagtaac tggaatgtta
      301 aaagttatgt acttgacaaa gatcatcatt gccctgcttc ttcttcgtgt acatttttct
      361 tcaggggaga tcagcttaat tatgatgatg tttacaggtt tggccaaatg gtggatgttc
      421 ttgctcttga attggaaaat gtgaacaaag aagcactaaa aaaactaaag caggaaggca
      481 aaaaaattta tcccgatcct gataaacttg aaattattca ggataaagga ttacaaaaac
      541 aattttatca aaaaataaaa gttccatcat caccattctc actttttaat agcaagcagg
      601 aaattctttc agccgttgac tcgggcaagt tacaattacc atttgttcag aaactccgca
      661 agggcggtta tgatggtaga ggtgtttcaa ttatccgatc taaagaggat ctgattaatt
      721 tgcttgatgg ggcgtctgtt attgaacatc tggttgatat agaaaaagaa atatctgcaa
      781 ttgttgcccg caatgaacgc ggagaagtga aatgtcatcc gacagttgaa atggaattta
      841 atccgaacgc caatcttgtt gaaaaattaa tttgtccctc tactttaagt aaagagaccg
      901 aagaagaagc agaaaaaatt gcagttaaaa ttattactga gttggatctt tatggaatac
      961 ttgctgtgga aatgtttgtt gataagaata aaaatatttg ggtaaatgaa gttgcgccac
     1021 gcccacacaa tagtggtcat catacaatcg aaagtgcaat cacctcgcaa tttgagcagc
     1081 tgttacgtgc aatttttaat cttcctcttg gcagcacaaa attaaaaatg cccgctgtaa
     1141 tgataaatct tttaggagaa tccggttatg aaggtaaagt cagatacgaa ggtttaacag
     1201 aatgcatggc agttgaggga gtaaaaattc atctttacgg aaaaaaaatt actaaacctt
     1261 tcagaaaaat gggacacgtt actgtgctgg ctccaactat tgatgaagcg aaaaagaaag
     1321 ctgattttgt aaaagaaaaa atgaaggtta tatcatgaaa aatattttag taagcataat
     1381 tatgggatct gattctgatt tagaagtaat gaaggaagcc ggaaaagttt taaaggaatt
     1441 tgacgtttct tttgagataa cagtcgtctc agctcatcgt acaccaaaac gacttgttga
     1501 gttttctgag aatgctcata aaagaggggt gcaggttata atagcaggag cgggaggcgc
     1561 tgcgcattta cctggaatgg ttgctgccat ttctcccctg cccgttattg gtgttcctgt
     1621 aaaatcttcc aactcacttg atggatggga ttctatactt tctattttac aaatgcccgg
     1681 tggagttccg gttgctacag tcgcactaaa taacgcaaag aatgccggat tactagctgc
     1741 acaaatgctt tctgttgcag atgaaaatct gaggaataaa gtaatctctt ataaagagaa
     1801 acttaactca gatgtgttga aaaagatcga taaggtagaa aagagtttcg aataaatatt
     1861 ttaaaaaagt ttctgtagaa gagaaaagct tttttgtgat ataagcggaa gatctcttgg
     1921 agaggttgca ggcgacataa aaaattttgt ttcaagaatc gaacttcctc caaatacaac
     1981 tattgaattt gccgggcaag ttgaacagca aacagatgca tttaaagatt tactgttact
     2041 tctcggatta agtattattc tggtttacat
//
LOCUS       DASWBY010000810         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_369568, whole genome shotgun sequence.
ACCESSION   DASWBY010000810 DASWBY010000000
VERSION     DASWBY010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369568"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VF870_14670"
     CDS             <1..928
                     /locus_tag="VF870_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253485.1"
                     /translation="HTWSAVKKVNDDNTTRHQFFTWMAIDQTTGILYFVFYDRRNTSN
                     NLTDVYVARSTDGGETFENFKVSESSFNPSSNVFFGDYTNIAAFNKKIYPIWMRLDTS
                     TLTVWTAIINDSSSVIPVELNNFFANVSDRNVNLYWQTSSELNNQGFEIQRLKDSKIE
                     KLKNWVSIGFIKGKGTTTDISDYSFVDEPISSGFYSYRLKQNDFGGSYKYSNVIDVNF
                     IIADDFRLSQNYPNPFNPSTTIEYQIPQSSFVTIKVYDALGKEVVTLVNEEKPAGIHE
                     VNFEPKDLTSGLYLYKIKAGSFEQTRKMILLK"
     gene            1130..>1531
                     /locus_tag="VF870_14675"
     CDS             1130..>1531
                     /locus_tag="VF870_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253486.1"
                     /translation="MKISATLVTILFVLIMVSMVSSRPDYNGATPGCAGSSCHTSQTG
                     IVTATVLTGNQVRITLTGTTGNVAGELVNSSGVVVAVINSTSSNPFTLNAPSAGSYTV
                     NAGFKNPSRQWGTTTVNIVAAPDAPSLLNSNP"
BASE COUNT          534 a          244 c          274 g          479 t
ORIGIN      
        1 acatacatgg agtgccgtta aaaaagttaa tgatgataac acgacaagac atcagttttt
       61 tacctggatg gcaatcgatc aaactaccgg aattttatat tttgtttttt atgatagaag
      121 gaatacttcg aacaatttaa ctgatgttta cgttgcccgt tcaactgatg gcggagagac
      181 ttttgaaaac tttaaagtaa gtgaatcatc ctttaaccct tcatcgaatg ttttctttgg
      241 tgattacaca aacattgcgg catttaataa aaagatttat ccaatctgga tgagattaga
      301 tacaagcact ttaactgttt ggacggcaat aattaatgat tcatcatctg ttattcctgt
      361 tgagttaaat aatttttttg caaacgtttc tgatagaaat gtaaatcttt actggcaaac
      421 atcgagtgaa ctaaataatc aaggatttga aattcaaaga ttaaaagatt caaagataga
      481 aaaattaaaa aattgggtat cgattggatt tataaaagga aaagggacca caaccgatat
      541 ttcagattat agttttgtag atgaaccaat atcttccgga ttttattcat acagattaaa
      601 acaaaatgat tttggcggaa gttataaata ttcaaatgtg attgatgtta attttataat
      661 tgctgatgat ttcaggttgt cgcagaatta tcctaatccg tttaatccga gtacaacaat
      721 tgaatatcaa attccgcaat catcttttgt aaccataaaa gtttatgatg cactaggtaa
      781 agaagttgta actttagtta acgaagaaaa acctgccggc attcacgaag taaactttga
      841 gccaaaagat ttaacgagcg gtttgtatct ctataaaata aaagctggaa gttttgaaca
      901 gacgaggaag atgattttgt taaagtagac tttccttaag taacacctta actgtatttt
      961 ataataagcc gtcagaaatg gcagtttttt atcaaaaatg aaaacaattc gtttctcttc
     1021 tctctacaat gataattgag ataaaacaat aataggaaat tgacaattgt ttattcggta
     1081 tggtatttgc gacttacaaa caattagtaa atatcaaaaa ggaacaaata tgaaaataag
     1141 tgccacatta gtaacaatat tgttcgtttt aattatggtt tcaatggtct catccagacc
     1201 agattataat ggagctactc ccggctgtgc cggatccagc tgccatactt ctcagacggg
     1261 aatcgtgact gcaacggttt taactggcaa tcaagtacgg ataacattaa caggaactac
     1321 aggcaacgtc gctggtgaac tggtaaattc aagcggagtg gtagtggcag ttataaattc
     1381 taccagcagt aatcctttta cacttaatgc tccttcggca ggcagttata ctgtcaatgc
     1441 gggttttaaa aatcctagtc gtcaatgggg aacaacaact gtaaacatag ttgctgcacc
     1501 agatgcccca agccttttaa attcaaatcc a
//
LOCUS       DASWBY010000811         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_597831, whole genome shotgun sequence.
ACCESSION   DASWBY010000811 DASWBY010000000
VERSION     DASWBY010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597831"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VF870_14680"
     CDS             <1..1163
                     /locus_tag="VF870_14680"
                     /inference="COORDINATES: protein motif:HMM:NF024500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HEX9253487.1"
                     /translation="GNVIAKQDTLTIKTPRGFYYGNLAKTRSNAGIYLDDKKVILTAD
                     SGEYFFNEDRAFFESKVKLYDTTTTLTSNELTYFQQQNRAVAVGDVTIFDGENEIIAD
                     TLEHFRNTKITFAFSNVRLINLKNNSEIYGEHLEDYRERGYSLIDVKPLFLQVDTTYT
                     ENEDGTKESKLDTLFIKSEVMEAYRDSSQRFFAIDSVSILKGEFASKNDLTVFYREKD
                     IIITKKINETTKQPVLWQENSQLTGDSITIYLNDNNIKQLDVDGTSFMLSQNEYNPER
                     YDQTSSAQIKIFFADNKIIRAEFYGNVYSIYYMYDGETANGLTKSNSAETTVLFEDNK
                     VAEVKLYKNPASEYYPEKQVSGNEKSYLLPRFVVLQDKPSKEEMYKLLNELR"
     gene            1169..1906
                     /gene="lptB"
                     /locus_tag="VF870_14685"
     CDS             1169..1906
                     /gene="lptB"
                     /locus_tag="VF870_14685"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX9253488.1"
                     /translation="MSTTLRSENLVKIYKKRIVVNKTSVQVSKGEIVGLLGPNGAGKT
                     TTFYMITGMIKPESGKVFLDDADITKVPMYKRARMGIGYLPQEASIFRTLSVEDNLLA
                     VLEMTNLNAKQRKEKCESLLEELTISHIRKSKGFQLSGGERRRTEIARALATDPSFIL
                     LDEPFAGVDPIAVEDIMNIVSNLKNRGIGVLVTDHNVHETLSIVDKGYILINGVIFKQ
                     GTADELANDEEVRKLYLGEKFKLDRYS"
BASE COUNT          724 a          283 c          369 g          601 t
ORIGIN      
        1 tcgggaatgt tattgcaaaa caagacactt taacgataaa gactccacgt ggattttatt
       61 atggtaatct agctaagaca agaagtaatg caggaattta tcttgatgat aaaaaagtaa
      121 tcttgacagc cgatagtggc gagtatttct ttaacgaaga tcgtgcattc tttgaatcaa
      181 aggtaaaact ttacgatacg acgaccacac ttacatcaaa tgaactgact tacttccagc
      241 agcagaatag ggcagtagcg gttggagatg ttactatatt cgatggagaa aacgaaatta
      301 tagcggacac gcttgagcac tttagaaata ctaaaataac ttttgctttt agtaatgtta
      361 gattgataaa tcttaaaaat aattctgaaa tttatggtga acaccttgaa gattatcgtg
      421 aaagaggcta ttcattgatt gatgtaaaac cattgtttct tcaggtagat acgacttata
      481 ctgaaaatga agatggaacg aaagaatcaa aacttgatac attattcatt aaatcagaag
      541 ttatggaagc atatcgcgat tcatctcaaa gattttttgc tattgattca gttagcattt
      601 taaaaggcga atttgcatca aagaatgatc ttactgtttt ctatagagaa aaagatataa
      661 taattacaaa aaaaataaat gaaactacaa agcagccggt actctggcag gaaaattctc
      721 agttaaccgg agattcaatc acaatttatc ttaatgataa taacatcaaa cagctagatg
      781 ttgatggtac atcgtttatg ctctcacaaa atgaatataa tccggaaaga tatgatcaaa
      841 cctcaagcgc gcagataaaa atattttttg cggataataa aattattaga gccgaatttt
      901 atggtaatgt ttactcaata tattatatgt atgatggaga aacagcaaac gggttaacaa
      961 aatcaaactc ggcagaaact actgtattgt ttgaggataa taaagttgct gaagttaaac
     1021 tttataaaaa tcctgcaagt gagtactatc ccgaaaagca ggtaagtgga aatgaaaaat
     1081 cgtatctttt accacggttt gtggtattgc aggacaaacc aagtaaagaa gaaatgtata
     1141 aacttttgaa tgagcttaga taaaaattat gagtactacc ttaagaagtg aaaatcttgt
     1201 aaagatttat aagaaacgaa ttgttgttaa taaaacttca gtacaggttt ctaaaggaga
     1261 aattgttgga ttgcttggac caaatggtgc aggtaaaaca acaacatttt atatgataac
     1321 cggaatgata aaacctgaat caggtaaagt ttttcttgat gatgctgata ttaccaaagt
     1381 cccgatgtac aaacgcgcaa gaatgggaat cggttattta cctcaggaag cttccatctt
     1441 cagaacttta tctgttgaag ataatcttct tgctgttctt gaaatgacaa atttgaatgc
     1501 taagcagcgt aaagaaaaat gcgaatcact tcttgaagaa ctaactatat cgcatattag
     1561 aaaaagtaaa ggatttcaat taagcggcgg tgaaagaaga agaaccgaaa ttgcacgcgc
     1621 acttgcaaca gatcccagtt ttattttatt ggatgaacca tttgctggag tcgacccgat
     1681 agcagttgaa gatattatga atattgtttc taatctcaaa aatcgcggta ttggtgtttt
     1741 ggttactgat cataacgtac acgaaacatt aagcattgtt gataaaggtt acatacttat
     1801 taacggtgta atatttaaac aaggcaccgc agatgaactt gctaatgatg aagaggttag
     1861 aaaactttat ttaggtgaga agtttaagct ggataggtat tcttaatttt attgtctttt
     1921 tacttttatt aaatatcctt tgttgtttga aaaacctgtc ataacggcca aatcttt
//
LOCUS       DASWBY010000812         3189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_748326, whole genome shotgun sequence.
ACCESSION   DASWBY010000812 DASWBY010000000
VERSION     DASWBY010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748326"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VF870_14690"
     CDS             complement(<1..373)
                     /locus_tag="VF870_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloenzyme"
                     /protein_id="HEX9253489.1"
                     /translation="MKSVLMIFIDGVGIGEKDYETNPFFKYGFKIFDELFKDTPHSQN
                     LFLEKDEMYVFPTDASLGVEGLPQSGTGQVSIFCGMNAPKFVGKHFGPFPYSTTIPII
                     EEKNIFRTFKDLGKSTYFANAY"
     gene            complement(370..834)
                     /gene="dtd"
                     /locus_tag="VF870_14695"
     CDS             complement(370..834)
                     /gene="dtd"
                     /locus_tag="VF870_14695"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX9253490.1"
                     /translation="MIALVQRVSEAGVYIAKKNFKSEIGKGMVILLGIKSDDSEIETN
                     FVADKCSGLRIFEDENEKMNLSLKDIDGEVLIISQFTLYGDAQKGNRPSFIEAARPEV
                     AIPLYEKFINRMRINLSEEKIKTGIFGEMMQVKIMNDGPVTIIVESKKQVNK"
     gene            complement(831..1685)
                     /gene="prmC"
                     /locus_tag="VF870_14700"
     CDS             complement(831..1685)
                     /gene="prmC"
                     /locus_tag="VF870_14700"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX9253491.1"
                     /translation="MITVLEVIKLSTEYLQKKGIESPRANAEILLAEILKCKRLELYL
                     SFDKPLAENEVQLYREAIRKRGLRIPLQYIIGNVEFYGLKLFVNENVLIPRPETELLV
                     EKIINESDKSVNLNILDIGVGSGNISLSILKNLPNSKVVAIDISENALNVAKQNAELN
                     LLQDKIEFKLFDIMNDDLNILGKFDLIVSNPPYVSENDYESLEPELKIHEPKIALTDN
                     SSGISFYKRIVEISDQIINKPGKIYFELGMGQSAQVQKIFEERNINNVSITKDYAGID
                     RIISGEII"
     gene            complement(1853..2887)
                     /locus_tag="VF870_14705"
     CDS             complement(1853..2887)
                     /locus_tag="VF870_14705"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEX9253492.1"
                     /translation="MEIKEYVKKYDPQNQFEVLINSYKQIEFAWNNQIKIDEIKSNEL
                     SNIIISGLGGSAISADLIKNFLGKEIEIPIIVNRNYNLPKFAGRNTLFIASSYSGNTE
                     ETISALYEAIEIGCKIICITTGGEVGEIVKKKNISCVIVQPGFQPRYSLGLSFFSLLK
                     VFQELKLIGDQTKIVEKVISLWKQKGIEYSHDGNYACTFAESLVGFIPVIYSVADTTS
                     AVGYRLKCQFNENSKLHAFHNEIPEMNHNEIIGWESYQEKVFHSKIINIIDEAYHPQI
                     QKRFEILKDIFSNSGVETLVLKSNEESYKVRLLDLIYLGDWISYYLGVLRGYDPSEID
                     NIYTLKERLK"
     gene            complement(2890..>3189)
                     /locus_tag="VF870_14710"
     CDS             complement(2890..>3189)
                     /locus_tag="VF870_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX9253493.1"
                     /translation="KTIDHIVKSAKESNIPVSICGEMAADTLAIPLLVGLGLDSLSMS
                     PGTILYAKRIIRGFQFKKAKALAEECLQCKTEVEVQKKVTKFFDDNNISRTRNIL"
BASE COUNT          999 a          596 c          428 g         1166 t
ORIGIN      
        1 gataagcgtt tgcaaaatag gttgacttac caagatcctt aaatgttctg aaaatatttt
       61 tttcttcaat tataggaata gttgttgaat atgggaaagg accaaaatgt tttccaacaa
      121 actttggagc attcattcca caaaatattg atacctgtcc agttccactt tgcggtaatc
      181 cttcaactcc caaactcgca tcagtcggaa aaacatacat ttcatctttt tctaaaaaaa
      241 gattttgaga gtgcggtgta tctttgaata attcatcaaa aattttaaat ccatatttaa
      301 agaacggatt ggtttcataa tctttttcac cgattccaac cccatcaata aatatcatca
      361 gcacagattt cacttattca cctgtttctt cgattcaact ataattgtca cagggccatc
      421 attcataatt ttcacctgca tcatttcacc gaatattcca gttttaattt tttcttcact
      481 aagatttatt ctcattctat taataaattt ttcataaagc ggaattgcaa cctcaggccg
      541 tgcagcttct ataaaacttg gacgattacc tttttgtgca tcaccataaa gtgtgaactg
      601 agaaataata agtacttcac cgtcaatatc ttttaaagat aagttcattt tttcattctc
      661 atcttcaaat attcttaatc ctgaacactt atccgcaaca aagtttgttt caatttcact
      721 atcgtctgat ttgatcccga ggagaattac catccccttt ccaatctcag atttgaaatt
      781 ttttttggct atataaactc ctgcttcaga aaccctttga acaagtgcaa tcatataatt
      841 tctccggata taattctatc tattccagca taatcttttg ttatagaaac attattaata
      901 tttctttcct caaatatctt ttgcacctgt gctgactgcc ccatacctaa ctcaaaataa
      961 attttaccgg gcttattaat tatttgatct gatatttcaa ctattcgttt ataaaatgat
     1021 atcccgctgg aattatcagt cagggcaatt tttggttcgt gaatttttaa ttcaggttct
     1081 aaactttcat aatcattttc agaaacgtac ggcggatttg acactatcaa atcaaattta
     1141 cctaatatat tcaagtcatc gttcatgata tcaaataact taaactcaat tttatcctgc
     1201 agcaaattca actctgcatt ttgttttgct acattcaatg cattttcact aatatcaatt
     1261 gcaacaactt tggagtttgg cagattctta agtatggata gagaaatatt tccactgccg
     1321 acaccgatat caagaatatt tagattaact gatttgtctg attcattaat tattttttca
     1381 acaagcaatt cagtttcagg tctggggata agtacatttt catttacaaa aagttttaat
     1441 ccgtagaatt caacattccc gattatgtac tgtaaaggaa ttctcaatcc tctctttcga
     1501 attgcttcac ggtaaagttg aacttcattc tctgcaagcg gtttatcaaa ggaaaggtag
     1561 agttctaatc gtttacattt tagaatttct gccagcagta tttcagcatt tgctcttggg
     1621 gattcaatac cttttttctg aagatattct gttgatagtt ttataacctc taaaactgta
     1681 atcatataat taatacttga attttaagaa attattaact cacattaaga aaattaatct
     1741 ttgactgtgc cttgagtgaa ttagttttct ttgattataa gttaacaatc atactatgca
     1801 cagccgaagt atgaacagaa gaatcaacat aaaaaaaagc ttacgtaatt ttttatttca
     1861 atctttcttt aagtgtgtag atattatcta tttcgctggg atcatatcct ctaagcaccc
     1921 cgagataata ggatatccaa tcaccgaggt aaattaaatc taacaatcta actttatatg
     1981 attcttcatt gcttttaaga accagagttt caactccgct gtttgaaaat atatccttaa
     2041 gtatctcaaa tcttttttgt atttgcggat gatatgcttc atcgataatg tttatgattt
     2101 ttgaatggaa aactttttcc tggtaggact cccatccaat aatttcattg tgattcatct
     2161 caggaatttc gttatgaaaa gcatgcagtt ttgaattttc attaaactga cattttagtc
     2221 tgtagccaac tgcactggtt gtatcagcga cagaataaat aacaggaata aatcccacta
     2281 aagattctgc aaaagtgcat gcataatttc catcatgaga atattcaatt cctttttgct
     2341 tccaaaggct gattaccttt tcaactattt tagtctgatc accaattagt tttaactcct
     2401 gaaatacttt caacagagaa aagaagctta aaccaagtga gtaccttggc tgaaaaccag
     2461 gttgaactat tacacaagaa atattttttt tcttaacgat ttctccaacc tcgccacccg
     2521 ttgtaatgca gataatttta catccaattt caattgcttc atataaagct gaaattgttt
     2581 cttcagtatt accggaatat gaagatgcaa taaatagagt gttcctgcca gcaaactttg
     2641 gaagattata atttctgtta actattattg gaatttctat ttccttacct aagaaattct
     2701 ttataagatc agcgctaatt gcagagccgc caagcccgct tataattata ttggataatt
     2761 catttgattt tatttcatca attttaattt gattattcca cgcaaattca atttgtttgt
     2821 atgaattgat caaaacttca aactgattct gcggatcgta tttttttacg tactctttta
     2881 tttccatagc tataaaatgt tccttgttct tgaaatatta ttgtcatcaa aaaactttgt
     2941 aactttcttc tgaacttcaa cttctgtttt acactgcaag cactcttctg ccaaagcctt
     3001 tgctttttta aactgaaatc cacgtatgat tcttttagcg tatagaatgg ttcccggtga
     3061 catactaagt gaatccaatc ctaaacccac aagtaacgga attgcaagtg tatctgctgc
     3121 catctcacca cagatactaa ccggaatatt actttctttt gcagatttaa caatgtgatc
     3181 aattgtttt
//
LOCUS       DASWBY010000813         5097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_668648, whole genome shotgun sequence.
ACCESSION   DASWBY010000813 DASWBY010000000
VERSION     DASWBY010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668648"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VF870_14715"
     CDS             complement(<1..290)
                     /locus_tag="VF870_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9253494.1"
                     /translation="MRKLIFDIETCSYPFETLAETQREYLLKYADKEIDPAKKEAMRG
                     EAIRYTSLYPFTAKCIAIGMYDVEKEKSYVYYESEKPEEWNSENTNVQYKGLP"
     gene            complement(375..668)
                     /locus_tag="VF870_14720"
     CDS             complement(375..668)
                     /locus_tag="VF870_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9253495.1"
                     /translation="MKSYYVYILTNKNNTVLYVGVTNDIVRRVWEHKSKLIEGFTKKY
                     NVDKLIYFEKCDNPLSAIAREKQLKAGSRKKKLDLINSFNKDWKDLFNLDQIY"
     gene            complement(714..1154)
                     /locus_tag="VF870_14725"
     CDS             complement(714..1154)
                     /locus_tag="VF870_14725"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9253496.1"
                     /translation="MDIKNFTHKTTIKVRFNEVDMLGVCNNAVYINFFEHARLEYVKE
                     AGLIPEGGIFSDGRLFFMVRNEINYRGHSFYDDELDVYSKVSYIKSSSFGYDHLIVRK
                     KTGEVIVDGKGVVVYVDPKTRKSTPLTEEFIEKIKKFEPCVQIL"
     gene            complement(1154..2176)
                     /gene="ltaE"
                     /locus_tag="VF870_14730"
     CDS             complement(1154..2176)
                     /gene="ltaE"
                     /locus_tag="VF870_14730"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEX9253497.1"
                     /translation="MIDLRSDTVTRPSEAMRKAMYNAEVGDDVFKEDPTVNKLEQYAA
                     ELLGKEAALYVSSGVMGNQICLNVLTNPGDEVICERDAHIFNYESGSPAALSGIQLFP
                     IEGNYGVITPEQVESIIRPTSAYYMPRTKVIEVENTHNRAGGTVWPIENIISLKHLAK
                     KYNLFYHLDGARIWNASVATGISVKEYASHFDSISCCLSKGLGAPVGSIIAGTKNFIK
                     EAYRVRKAWGGGMRQVGILAAAGLYALQNNIERLKEDHEKAKFLAERINANPNLEIDM
                     QAVQTNILLFHPKNISVEEGLKRCKDKGLLVSVGKIDLIRAITHLDVTFDEVKKAADI
                     IDEVFT"
     gene            complement(2277..3923)
                     /gene="lnt"
                     /locus_tag="VF870_14735"
     CDS             complement(2277..3923)
                     /gene="lnt"
                     /locus_tag="VF870_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX9253498.1"
                     /translation="MKSLFNRESISAEEKKHRRNDRLLLILSGVLFGISFTPFPFPFP
                     LFLLVAFVPYFFAIERRKTLLKVNSASYLTFFTMSLITVYWVGSWQSEADPFLMISGV
                     VLVFFLPCVMLINSTLYFLSRKVFKKDLSLYFFPFFWVTGEYILTLTDLKFPWLTIGH
                     GLAKFTSFIQIADIVGAFGLSFIVLWINIFIYKGLKSFKENVKVSTIYFSAAASIFIL
                     IIIYGFVKISSVNKEERKIKVGIIQPNIDPWNKWELGGLDEILNNYFSLSQKCVDEGA
                     KIILWPETALPVYLLSGTYQSEVDSIYLFLKKNNVSLLTGMPDFIIYNENAPRNAKYS
                     EVGKYHYVTYNSILLLQPNTDNIQRYGKMQLVPLGEHTPFVDQLPFLGDLLKWGVGIS
                     GWNVGQDTTVFKVVTDKDTIKIGGLVCYESVFPTFPNYFVDRGAEFLTVLTNDSWYGK
                     LSGPYQHKEFANLRAVENRRAVVRCANGGVSCLINKFGVTEVETEMFTRTHLVVDVPL
                     NEEKTFYTKNPLIIPVLSSAFSLWIFGINILLWMKKKFKL"
     gene            complement(3920..4531)
                     /locus_tag="VF870_14740"
     CDS             complement(3920..4531)
                     /locus_tag="VF870_14740"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9253499.1"
                     /translation="MFETILTQISNLTPFWIYLTIFFFAYIENLFPPSPSDIVVVIGG
                     SLVGTGTISFIPLFVSATIGSLAGFLTAFIIGWQFDKKLIHSGKIKFINIQSIDKVES
                     AFRKWGYYLIVANRFLPGTRAVISFFAGMSRLNVNKTSILSLLSSALWNLILIYLGIT
                     FGKNIDLIDNYLKTYSTIIWVITGIIILFFVVRYFIIKRKKSN"
     gene            complement(4524..5021)
                     /gene="ispF"
                     /locus_tag="VF870_14745"
     CDS             complement(4524..5021)
                     /gene="ispF"
                     /locus_tag="VF870_14745"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX9253500.1"
                     /translation="MKLNFRTGFGFDVHAFAEGRKLIIGGVEIPFEKGLEGHSDADVL
                     LHAICDAMLGALALGDIGMHFPNTDEKWKDADSSILLERVNVIINSNGYELGNLDCVL
                     AMEKPKISPYVDQIRKRISEILSADTNQISIKATTAEKLGFVGRTEGVVSFATVLLIK
                     KDINV"
BASE COUNT         1682 a          976 c          753 g         1686 t
ORIGIN      
        1 ggcaaacctt tgtattgcac atttgtgttt tcagaattcc attcttcagg tttttctgat
       61 tcgtagtaaa cgtatgattt ttctttttct acatcataca ttccaatggc aatacatttt
      121 gctgtaaagg gataaagtga tgtatagcgg attgcttcgc ctctcattgc ttcttttttg
      181 gcgggatcaa tttctttgtc ggcatatttg agcagatact cacgctgagt ctcagctaaa
      241 gtttcaaaag gataagaaca agtttcgata tcaaagatta gtttgcgcat tttagcctcc
      301 caataattta aagttgtcat tgcgaatccc gatcttgcct gccggcagac aggtaaatcg
      361 ggtgaagcaa tctctcaata aatttgatct aaattaaata aatctttcca atctttatta
      421 aaggaattaa ttaaatccaa tttctttttt cttgaaccag ctttcaactg cttttctctg
      481 gcaatcgcag aaagtggatt atcacacttt tcaaagtaaa taagtttatc aacgttatac
      541 tttttagtaa aaccttctat caatttcgat ttgtgttccc aaactctcct aacaatatca
      601 tttgtaacac ccacataaag aacagtatta tttttatttg ttagtatata gacgtaataa
      661 cttttcattt ttattacaga ttgcttcact cgaaggctcg ttcgcaatga ctattacaag
      721 atctgcacgc aaggttcaaa ctttttaatc ttctctataa attcttctgt tagcggagtt
      781 gattttctgg ttttaggatc aacgtaaaca actactcctt ttccatcaac aattacttcc
      841 cctgtttttt ttctaacaat caaatgatca tatccaaaag aagagctttt tatgtaagat
      901 acttttgaat aaacatcgag ttcatcatca taaaatgaat gacctcgata atttatttca
      961 tttcttacca taaagaataa tctaccgtca gaaaatattc caccttcagg aattaatcca
     1021 gcttccttaa catattccag ccgtgcatgc tcaaagaaat taatgtaaac tgcattgttg
     1081 caaacgccaa gcatatcaac ttcgttaaag cgaaccttta tagttgtttt atgagtaaaa
     1141 ttttttatat ccattaagta aacacctcat caattatatc cgcagctttt ttcacttcat
     1201 caaaggttac atctaaatgt gtaattgcac ggattaaatc aatctttcca actgaaacaa
     1261 gcaatccttt atctttacat cgtttcaatc cttcttcaac ggaaatattt ttaggatgaa
     1321 ataataaaat gtttgtttga actgcctgca tgtcaatttc cagatttgga ttagcattaa
     1381 ttctttctgc aagaaattta gctttctcgt gatcttcttt taatctctca atattatttt
     1441 ggagtgcata caaaccagct gcagcaagaa ttccaacttg cctcattcct ccgccccagg
     1501 ctttacgaac acgataagct tctttaataa agtttttcgt tccggctatg atagaaccaa
     1561 ccggagcgcc aagtcctttt gatagacaac aggaaatcga atcaaaatgt gaagcatatt
     1621 ctttaacgga aattcctgtt gcaacagaag cgttccagat tcttgcgcca tcaagatgat
     1681 aaaaaagatt atactttttt gccagatgtt ttaacgaaat aatattttca atcggccaaa
     1741 ctgttccgcc agcacgattg tgagtgtttt caacttcaat aacttttgtt cgcggcatat
     1801 aataagcaga agtcggtcga attattgatt caacttgctc gggtgtaatt actccataat
     1861 ttccttctat cgggaataat tgtataccac ttagcgcagc aggcgagcca gattcataat
     1921 taaaaatatg tgcatctctc tcacaaataa cttcgtcccc gggatttgtt aaaacattca
     1981 aacaaatctg gtttcccata accccgcttg agacatacaa tgcagcttct ttgcctaaaa
     2041 gttctgctgc atattgttca agtttgttaa ctgtgggatc ttctttaaaa acatcatcgc
     2101 caacttcggc attgtacatt gctttgcgca ttgcttctga tgggcgtgtt acggtatcgc
     2161 ttcttaagtc tatcataatc ttcctggtaa gtcattgcga ggagagactc gacgaagcaa
     2221 tccaaacatt tatttataga agatcacttc gctctgctcg tgatgacaat taaattttat
     2281 aatttaaact tttttttcat ccacaataaa atgttaatcc caaaaatcca taaagaaaat
     2341 gcggatgata aaaccggaat aatcaatgga ttttttgtgt aaaatgtttt ctcttcattc
     2401 aacggaacat caacgactaa atgagttctt gtaaacattt ctgtttcaac ttctgtaaca
     2461 ccaaatttat ttatcaagca gcttactccg ccatttgcac aacgtacaac tgcccttcta
     2521 ttttctactg cccgtaagtt agcaaattct ttatgctgat atggcccgct taactttcca
     2581 taccagctat cattggttag aactgttaag aactccgcac cacgatcaac aaaataattt
     2641 gggaaagttg ggaaaactga ttcataacaa accaatccgc caatttttat tgtgtcttta
     2701 tcagttacaa ctttaaagac tgttgtatcc tgtccaacat tccagccgct gattcccact
     2761 ccccatttta atagatcgcc aagaaatgga agctggtcca caaaaggagt atgctctcca
     2821 agagggacca attgcatttt gccatagcgc tgaatattat ctgtattcgg ttgaagaagc
     2881 aaaattgaat tgtatgtcac gtagtggtac ttaccaactt cactatactt tgcattacga
     2941 ggagcatttt cattatagat tataaaatcc ggcattccag ttaaaagcga aacattattt
     3001 ttctttaaga aaagataaat tgaatcaact tcactttgat atgttccgga taataaataa
     3061 acaggcaaag cagtttcagg ccaaagaatt atctttgctc cttcatcaac acatttttgt
     3121 gataagctaa aatagttatt aagaatttca tcaagtccgc caagttccca tttgttccag
     3181 ggatcaatgt ttggctggat gattccaact tttattttcc tctcttcttt attaacagaa
     3241 gatattttta caaatccata gattataatt aaaataaaaa ttgaagcagc agcagaaaaa
     3301 taaatcgtac tgacttttac attttctttg aacgatttca atcctttata aatgaaaatg
     3361 tttatccaca aaacgataaa tgataaacca aaggctccaa cgatatctgc tatctgaatg
     3421 aaggatgtaa actttgcaag cccatgtcca attgttaacc acggaaattt gagatcggta
     3481 agagtaagaa tatattctcc agttacccaa aagaaaggga agaaataaag tgataaatct
     3541 tttttaaaaa cttttcttga taaaaaatac aaagttgagt ttatcaacat cacacaaggt
     3601 aaaaagaaga ctaaaacaac tccgcttatc attaaaaatg gatcagcttc gctttgccag
     3661 cttcctaccc aataaacagt tatcaaactc atcgtaaaaa aagtcagata agaagctgag
     3721 tttactttta gtaatgtttt tcttctttct atcgcaaaaa aatatgggac gaatgcaact
     3781 aatagaaaaa gcggaaatgg aaaagggaaa ggtgtaaatg aaatcccaaa aagaactccg
     3841 gataaaatca gaagaagtct atcatttctt cgatgttttt tttcttcagc agaaatggat
     3901 tctctgttaa ataaagactt caatttgatt tttttctttt aatgatgaaa tatctaacta
     3961 caaaaaacaa aattattatt cctgtaatta cccaaattat tgtgctgtat gtctttaaat
     4021 aattgtcgat aagatcaatg ttttttccga atgttattcc gagataaatt aaaatcagat
     4081 tccataatgc agaactaaga agactaagaa ttgatgtttt atttacatta agcctgctca
     4141 ttcctgcgaa gaaagaaatt acagctctag tgccaggcaa aaatctgttg gcaacaatta
     4201 agtaataacc ccattttcta aacgcagatt caaccttatc tattgattgt atattaataa
     4261 atttaatttt tccggaatga attaattttt tatcgaactg ccagccaata ataaaggcag
     4321 ttaaaaatcc tgccaggctt cctattgttg cagaaacaaa aagagggata aaagaaatag
     4381 ttccggtacc aaccaaagat cctccaataa caacaacaat atcacttgga gatggcggga
     4441 ataagttttc tatataagca aagaaaaata ttgtaagata aatccaaaat ggcgtcaaat
     4501 tagaaatctg agtcagaatt gtttcaaaca ttaatatctt tcttgatcaa taaaactgta
     4561 gcaaaagaaa caacgccctc ggtcctgccc acaaacccaa gtttttctgc agtagttgct
     4621 ttaatggaaa tttgattagt atctgcactt agtatttctg aaatacgttt tcttatttga
     4681 tccacgtaag gagatatttt gggtttttcc attgcaagca cacaatcaag gtttcccaat
     4741 tcgtatccat tagaattgat tataacattt acacgttcta ataaaattga actatcagca
     4801 tctttccatt tttcatctgt gtttggaaaa tgcattccaa tatcaccaag cgctaatgca
     4861 ccgagcattg catcacaaat tgcgtgaaga agcacatctg catcagagtg tccttctaat
     4921 cctttttcaa atggaatttc aactccaccg ataattaatt ttcttccttc cgcaaatgcg
     4981 tgaacatcaa aaccaaatcc agttctaaaa tttaatttca atatctaacc tcaaatcgtt
     5041 taaattcatt attatattca gaccaatcga ctgaacaatt tgttacgtga gatttca
//
LOCUS       DASWBY010000814         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_485126, whole genome shotgun sequence.
ACCESSION   DASWBY010000814 DASWBY010000000
VERSION     DASWBY010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485126"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2238
                     /locus_tag="VF870_14750"
     CDS             <1..2238
                     /locus_tag="VF870_14750"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX9253501.1"
                     /translation="GYLDLSVKGLDEDFSLFSLLLSDEGMKNIKSGDFVFEGSVKGKT
                     LIEFPQIEIAFGLKDVELVNPVTKRRIKHLNLEGDFNSGKSDDWSDASLIVDTLYGDL
                     QDGRISLSGSVRNLKEPNVDINVFLSADVTGLEKVFKLGSISNLKGKVELIDRIKGKY
                     LIDEKKFVNENNTGRLYLENFGVKIPETITFDKVNGVIRRDNNDFYFDSLNIISEDTD
                     ILINGKVQNLAYLFLGVEKDIEADLDIKSSVFNLPNFLAFDPSIKRDFNHRILDVDVS
                     VNAKTTTSKATKFKSFPELDFDIRKLDATVEDFLPRIEINSGNYKISESILGFNMKFD
                     QFKTDFMGGKFNFSAEYNTSKYQPFYIKMKTDFNKVYLPDLFYNQDDTIPESLSGKLS
                     GSFFTEFQFPTDSTVLKFIKIKNAELVYEYSKDTIVTKNLELELNKIYFNDKISSNPL
                     ATLYTNGRVKSDNFKSSSFNFRDLNFYLNVINGDYEINSKTVSIFGDDAKGQVKITVA
                     PFADVPKFNITYNDISFRAEKMLSAFNENQVISGPLEISFNISSSGSEWDTIVSNLDG
                     TINLSGKNLVLNGLDADEMIDKFKRSQNFNLVDLGAVVLAGPVGLVVTKGTNFASIFV
                     FNSGKSTIINQLASNWTISNGVFEIQDAAFTTNKNRIALTGQIGFSNSSLDLIIALLD
                     EDGCSIFSQEVSGSLDNPTIGKVKVVGTVLAPVTNLVDDVTGKDCDVFYSGSVKHPIN
                     KQKKN"
     gene            complement(2328..>2547)
                     /locus_tag="VF870_14755"
     CDS             complement(2328..>2547)
                     /locus_tag="VF870_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253502.1"
                     /translation="ENYTVYTPLIGQPYAESERNWVPFLLLGGGFVQHVGPNASVYVE
                     VLFDVIQDDNSPYENWDPIISIGGGVGF"
BASE COUNT          879 a          370 c          464 g          834 t
ORIGIN      
        1 ggttatctcg atttatcagt taaaggattg gatgaagact ttagcttatt ctcacttttg
       61 ttaagtgatg aaggaatgaa aaatattaag tcaggtgatt ttgtctttga aggttctgtt
      121 aaaggaaaaa cattaattga gtttcctcag attgaaattg ctttcggact taaagatgtg
      181 gagcttgtta acccagttac taaaagaaga attaagcatt tgaatctcga aggggatttt
      241 aactcaggta aatctgatga ttggtctgat gcaagcttaa ttgttgatac tttatatggt
      301 gatttacaag atggaagaat tagtctctca ggttctgttc gcaatttgaa agaaccaaac
      361 gttgatataa atgtttttct ttctgcagat gtaaccggat tggagaaagt gtttaaactt
      421 ggatcaattt ctaatttaaa agggaaggta gaactaattg acagaataaa agggaagtat
      481 cttatcgatg agaagaagtt cgtgaatgaa aataatacag gcagacttta tttagaaaat
      541 tttggggtga aaattcctga gacaatcaca tttgacaaag taaatggtgt aatcagaaga
      601 gataataatg acttttactt tgacagcctc aacataattt ctgaagacac tgatattctg
      661 atcaacggta aagttcagaa tcttgcttat ttgttcctgg gtgtggaaaa agatatcgaa
      721 gcagatcttg atattaaatc atcagttttc aacttgccaa attttcttgc ttttgatcca
      781 tcaataaaga gggattttaa ccacagaatt ctggatgttg atgtctcggt taatgctaaa
      841 accacaactt ctaaggcaac aaaatttaaa tcatttcctg aactagattt tgatatcagg
      901 aaattggatg caactgtaga ggattttctg cctcgtatcg aaattaattc cggaaattat
      961 aaaatcagtg aaagtatcct cggatttaat atgaaattcg atcagtttaa aactgatttt
     1021 atgggcggta aatttaattt tagtgcggag tataatacgt caaagtatca gccattttac
     1081 ataaaaatga aaactgattt caacaaagtt tatttaccgg atctttttta taatcaagac
     1141 gatacaatac ccgaatctct cagcggtaaa ctttctggtt cattcttcac agagtttcaa
     1201 ttcccgactg actctacagt attgaaattt ataaaaatca aaaatgctga attagtttat
     1261 gagtattcaa aggatactat agttaccaaa aacctggagc ttgaattaaa taaaatatat
     1321 tttaacgata agatcagctc gaatccatta gcaactttat atacaaacgg cagagttaag
     1381 tcagataact ttaaatctag ttcattcaac tttagagatc ttaattttta tttgaatgtg
     1441 ataaacggag attatgaaat taattcaaaa acggttagca tttttggaga tgatgcaaaa
     1501 ggacaggtaa aaataacagt cgcacctttt gctgatgtac ccaaatttaa catcacttac
     1561 aatgatatta gttttcgtgc tgagaaaatg ttgagtgctt tcaatgaaaa tcaggtaatt
     1621 agcggaccgc ttgaaatttc atttaatatt tcatcttcag gatctgaatg ggataccatt
     1681 gttagtaatt tggacggcac aattaatctc tctggtaaaa atcttgtgct taacggactc
     1741 gatgctgatg agatgattga taaatttaaa cgcagccaga attttaatct tgttgatctt
     1801 ggcgctgttg tgcttgccgg acccgttgga cttgtcgtaa ctaaagggac taattttgcg
     1861 agtatctttg tatttaattc aggaaagtct acaattatta atcaattagc ttctaattgg
     1921 acaatcagta acggtgtttt tgaaatacag gatgctgcat ttacaacaaa taaaaatcga
     1981 attgcattaa caggacagat aggattttcg aacagcagtc ttgatttaat aatagctctt
     2041 ttagatgaag atggatgcag tatttttagt caggaagtct ccggcagcct ggataatcct
     2101 acaataggaa aagtaaaagt tgttggaact gttttggctc ctgttacaaa tttagtagat
     2161 gatgttactg gaaaagattg tgatgttttt tacagcggct cggttaaaca tccgataaat
     2221 aaacaaaaaa agaattgata attgaaataa aattattaca ataatacaca gattataaaa
     2281 aaaagacaga ataatattta ttttcttatt ctgtctttag taattaatta aaatcctaca
     2341 ccgccgccaa tgctaataat tggatcccaa ttttcatatg gtgaattgtc atcctggata
     2401 acatcaaaca aaacttcaac atatactgaa gcattgggtc caacgtgctg tacgaatcca
     2461 ccacctaaca ataaaaaggg aacccagttt ctttctgatt ctgcatatgg ttgaccaatt
     2521 aatggagtat aaacagtgta gttctca
//
LOCUS       DASWBY010000815         6958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_598087, whole genome shotgun sequence.
ACCESSION   DASWBY010000815 DASWBY010000000
VERSION     DASWBY010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598087"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF870_14760"
     CDS             complement(<1..306)
                     /locus_tag="VF870_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX9253503.1"
                     /translation="MKAKSIKGKSPEEIQTALTDSMADGFKPTLAIVFMSIKQEREII
                     CALMNNLGIQIFGSTTSGEFINGDISEGGITIMLLDINPTYFKIVFLETGNSSVYEMS
                     "
     gene            complement(362..1192)
                     /locus_tag="VF870_14765"
     CDS             complement(362..1192)
                     /locus_tag="VF870_14765"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX9253504.1"
                     /translation="MEILYTLLNIDPNYIIIGLFVVFFTFEQILNTPFKFDKRPKHFF
                     HNFLFQILFVIANYFFAIFVVFSVEWLNDHNIGLLYLFDIPFWPKLIIGVLLYDVTTY
                     WIHRATHKIPLLWRFHRVHHSDTSVDSSTFFRFHPIELILVFGIGNILTVLLFGTDLL
                     SMALYYLILNIFVFFEHSNLYYPYWLDKTFGWIITTPNLHKVHHEQTQFYTDSNFADI
                     FILWDRLFGTYKYLPVENINYGLKEFDEDKKQTFQYLILSPFINIKRITSEKAVNSDQ
                     "
     gene            complement(1253..4186)
                     /locus_tag="VF870_14770"
     CDS             complement(1253..4186)
                     /locus_tag="VF870_14770"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9253505.1"
                     /translation="MNAKKEKLLNVTYHKWMQAGLYDLPLAEINEYIDPKIMGYGTAI
                     DEKVLSISDYRELIKTQREQAVGLDMSIEVTPVFRSISNKEDSAIFVDEITVCIVANG
                     NKQKLFLRITTMLEYCDRNWKVVHWHGSIPTETTGETDTWLVNEWKRKNEELQKLVEE
                     KTSELLDKNRELEIESSLEKVRSVAMAMREPADMLDVCQTISLQLKSLGIKEIRNVQT
                     AIFYEQSGTYMNYEYYAKHKKTIITETSYTNHDMHIEFARKMLKGKGEFFVTHIKGKK
                     VKDWIAYQKTTNVFIDKYLETASSINYYWFSLGPVALGMSTYSPLADEEINLFKRFVK
                     VFELAYQRYLDIEKAEAQAKEAEIELALERVRARTMAMQKSEELNDVVMTLFGQMKDL
                     EIDIDGININILNDQLTGFDSWFTAPGYTKAVCMFTPYFDTPVMNDIFNAVKSKQDLL
                     SKIYQKKEKDAYFSYLYEHSDYKNLPDERKKMILGAEGWCLSVGIANNIGISIHSYTD
                     RSFSEKENDILKRFAKVFEQTYTRFLDLQKAEVQAKEAEIELALERVRARTMGMQKSD
                     ELKEVIKIVYQQLIQLNIKLDHTGFVVDYTPGGEWHFWIADEQDIPAKITHPYFESVW
                     ANQFNEAKEKGEDFFVTNLNFEQKNKFYQELLSHIPGLPEKSKEFYLNCPGLAATTVV
                     LDNIGLYIENFEGIPYTYEENNILMRFGKVFQQTYTRFLDLQRVEAQNKIIQAENERK
                     SKELEDARQLQLSMLPKELPKLPHLDISVYMKTATEVGGDYYDYNVDADGTLTFVVGD
                     ATGHGMMSGMMVSIMKSFFISNKSNLELKTFFESANNSIKEMQLGRLMMACMGVQITS
                     EKIIATNAGMPSLIYFRNKSQKAGEFVSHNFPLGGMKGTIYSLKKIRYEKGDTLLLMS
                     DGFAELRNDKNEQYGYPRIIKEFKSVVQKSSTEVVEYLKNSASQWINGLDPDDDVTFV
                     VIKVK"
     gene            complement(4221..>6958)
                     /locus_tag="VF870_14775"
     CDS             complement(4221..>6958)
                     /locus_tag="VF870_14775"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9253506.1"
                     /translation="SKMNKLFMTTLIRGGEIFNGKNKSVFEFENGKWKVSNILQEINA
                     IRDLKTDRVINDFKYQSIFFMKSSKYFPERLVFQVTALNQSSKYLNQLFSFENNKYKK
                     FDSFESGPAVALSEGVAMKTSKGIGIYYPNYSSMLTSKDGLLVTQSGIVTPYTDYSGL
                     VWFSYSYTDLPAYALTVDVGINVWDGKYLRTITEKDGLASNITFKTFQDSKKRVWITT
                     SKGLTLIREIRNSEGEQIFKINSIPNEDGKPYSTSNVLETKNGDIYTWQKYIRPASDN
                     LIGADPYFGRLEGDKLITIKSPFDETDNAKKYQLFDIKQDNEGRLWLFGIFSDNIKDI
                     TSVQSKIMLFDGKSWSKPPSSWNVPSEQLHYVGNLKNGMYFLTVGGFYVFNGNNFVNL
                     SDSVNENADFRILKGASVAGTATEIQAGEYLYIRLRNRGLVIFDGTNLKFYTKKDGLL
                     STNLSNPIIDEIREAVYFSSPSGALKISGNKFQTFYHDESVASGGPYISAMDGFGNML
                     EFYNGVGLYINKSEEKSYPLLISSVSVTGKSYFYNLPKELPYSENSFIFNYAALNYKD
                     PKQTTYEHFLEGFDKGWSKASTLPFAEYQSLPFGKYTFRVRGVTANGVKTNEASYSFR
                     VNPPIWRTWWAYGLYFLGFVGMLGGVRKYELERRKENENNKFLQIENDRKTKELEEAR
                     QLQLSMLPKALPSVPHLDIAVFMKTATEVGGDYYDFHVHMDGTLTIVLGDATGHGMMS
                     GMMVSIMKSLFMSDRTNKELKPFFENANEAIKDMQLGRLMMALTCVQISNNKVVTTNA
                     GMPPLFIYRKYSQTIEEVVINNMPLGAMKGNAYDIKEFKIERGDTLLLMSDGFAELKN
                     ENQEMYGYKRARNSFEESAKKEPEEIISDLRTEARSWTNDKEPDDDVTFVVIKVK"
BASE COUNT         2128 a         1307 c         1028 g         2495 t
ORIGIN      
        1 tgacatttca taaactgaac tgttacctgt ctctagaaaa actattttaa aataagtagg
       61 atttatatcg agtaacataa tcgtaatacc accttcgctg atatcaccat taataaattc
      121 acctgatgtt gtagaaccga aaatctgtat tcccagattg ttcattaatg cgcaaataat
      181 ttccctctcc tgttttatag acataaaaac aatggcaaga gttggcttaa aaccatcagc
      241 catagagtct gttaatgctg tttgaatttc ttcgggtgat tttcctttaa ttgattttgc
      301 tttcattctc tttttatttt ttgagtatta tgaaatttaa aatataatta caatatttta
      361 attattggtc gctattaact gccttttcgg aagtaatgcg ttttatgtta ataaaaggac
      421 ttaatatgag atattggaaa gtttgctttt tgtcctcatc aaattctttt agtccatagt
      481 taatattttc cactggtaag tatttgtatg ttccaaacag ccggtcccat aaaataaaaa
      541 tatctgcaaa attggaatcc gtataaaatt gagtttgctc atggtgaacc ttatgaagat
      601 ttggagttgt tataatccat ccaaatgttt tatcgagcca gtaaggataa taaaggtttg
      661 aatgctcaaa gaaaacaaaa atgttcaata ttagatagta gagcgccatt gatagtaaat
      721 cagttccaaa taacagtact gttaaaatat tgcctattcc aaaaaccaat attagttcaa
      781 ttggatggaa cctaaaaaat gtagatgaat ctactgaagt atcactatgg tgtacacgat
      841 ggaatctcca caacaatgga attttatgtg ttgctctgtg aatccagtaa gttgtcacat
      901 catataataa aacacctatt ataagttttg gccagaaagg tatatcaaac aagtataata
      961 gaccgatatt atgatcattt aaccactcaa cactaaacac tacaaatatt gcaaaaaagt
     1021 agttcgcaat aacaaagaga atctgaaaaa gaaaattatg aaaaaaatgc ttcgggcgtt
     1081 tatcaaattt aaagggtgta tttaaaattt gttcaaaagt aaaaaaaaca acgaacaaac
     1141 cgataataat ataattcgga tctatattaa gtaatgtata tagtatttcc atttttctcc
     1201 ttctataaat gattgtgctt ttctaatttt ttcatttaca attttattaa tatcatttta
     1261 cttttatcac cacaaaagta acatcatcat cgggatctaa tccgttaatc cactgagatg
     1321 ctgagttctt taaatactca actacttcag ttgagctttt ttgcactacg gacttaaatt
     1381 cttttattat tcgaggataa ccatactgtt cattcttatc attcctcagt tcagcaaatc
     1441 catcactcat aagtaaaagc gtatcacctt tttcatatct aattttcttc aaagagtata
     1501 tagtcccctt catccctcct aatggaaaat tatgactaac aaattctcct gccttctgag
     1561 atttatttct gaaataaatt aaagatggca ttcctgcatt tgtcgcaatt atcttttcgg
     1621 atgtaatttg aactcccatg caggccatca tcaatcttcc aagctgcatt tcttttattg
     1681 agttattggc gctctcaaaa aacgtcttta attctaaatt ggatttattt gaaatgaaaa
     1741 acgatttcat aattgaaacc atcattccgg acatcattcc gtgtcctgtt gcatctccaa
     1801 caacaaatgt aagtgttcca tccgcatcaa cattataatc atagtaatca ccaccaacct
     1861 ctgtagcagt tttcatatag acagaaatat cgagatgtgg aagtttagga agttcttttg
     1921 gcagcataga tagttgaagt tgacgcgcat cttcaagctc tttggattta cgctcatttt
     1981 ccgcctgaat aattttattc tgcgcttcta ccctttgtaa atcaagaaaa cgggtatagg
     2041 tttgttggaa tacttttcca aagcgcatta gtatattatt ctcttcatat gtataaggta
     2101 taccttcaaa gttttcgatg tacaggccta tattatccaa caccaccgtt gtagctgcaa
     2161 ggccagggca gtttaaataa aattcttttg acttctcagg taaccctgga atatgggata
     2221 aaagttcctg ataaaattta ttcttttgct caaaatttag gttggtaaca aagaaatctt
     2281 cgcctttttc ttttgcctcg ttgaattgat ttgcccaaac agactcaaaa tatggatgag
     2341 ttattttagc aggaatatcc tgctcatcag ctatccaaaa atgccattcg cctccaggtg
     2401 tatagtcaac aacaaaacct gtatgatcga gcttaatatt taactgaatt agttgctggt
     2461 aaactatttt gattacttct ttaagctcat cgcttttttg catacccatt gttcttgcac
     2521 ggaccctttc aagcgctaat tcgatttcag cttcttttgc ctgtacctct gctttttgaa
     2581 gatcaaggaa gcgggtatag gtttgttcaa atactttggc aaaacgtttt aaaatatcgt
     2641 tttccttttc tgaaaatgac ctatcagtgt agctatgtat acttatgcct atattatttg
     2701 caattcctac agacaggcac cagccttcag cgccaagaat catttttttc ctttcatccg
     2761 gcaggttttt ataatcagaa tgttcataca gataagagaa gtaggcgtct ttttcttttt
     2821 tttgataaat cttactgaga agatcctgtt tgcttttaac agcattaaaa atatcattca
     2881 ttacaggagt gtcgaagtaa ggagtaaaca tgcagacagc ttttgtataa ccaggagctg
     2941 taaaccagga atcaaaaccg gttagttggt cattcaagat gttgatatta attccatcaa
     3001 tatcaatttc caaatctttc atttgaccga ataatgtcat tactacatca ttaagttctt
     3061 cacttttttg cattgccatt gttcttgcac gaactctttc aagcgctaat tcgatttcag
     3121 cttcttttgc ctgagcttcg gctttttcga tatcaagata cctttgatat gccagttcaa
     3181 acactttcac aaatcgtttg aataaattaa tctcttcatc agccaatgga gagtaagttg
     3241 acattcccaa agcaacaggt ccgagtgaaa accaataata atttattgaa gaagcagttt
     3301 ccaggtattt atcaatgaaa acatttgttg ttttttgata agcaatccaa tctttaacct
     3361 ttttaccttt tatatgagta acgaaaaatt ctcctttccc tttaagcatt tttctggcga
     3421 attctatatg catatcatga tttgtataag atgtttcagt tatgattgtt ttcttgtgct
     3481 ttgcataata ttcgtaattc atataagtgc cactttgttc atagaagatg gccgtctgaa
     3541 cattacgaat ttcttttatg ccaagtgatt taagttgcag cgaaatagtt tgacacacat
     3601 ccagcatatc agcaggttct ctcattgcca tagcaacgga tcttactttt tcgagagatg
     3661 attcaatttc aagttcccgg tttttatcca acagttctga agtcttttct tcaacaagtt
     3721 tttgaagttc ttcatttttt cgtttccatt catttacaag ccaggtatca gtttcacctg
     3781 ttgtttcggt tggtatagaa ccatgccagt gaactacttt ccagtttcta tcacaatatt
     3841 ctaacatagt tgtaattctt aaaaagagct tctgcttatt accatttgca actatgcaaa
     3901 ctgtaatctc atcaacaaaa attgcggagt cttccttgtt cgatatactt ctgaaaacag
     3961 gagtaacttc aatactcata tcaaggccga ctgcctgttc tctttgtgtt tttatcaatt
     4021 cacgataatc ggatatggaa agtacttttt catcaatggc agttccataa cccataatct
     4081 ttggatcgat atattcattt atttcagcta gcggtaagtc atacaatcct gcctgcatcc
     4141 atttatggta agtaacgtta agtaattttt cttttttggc gttcataatt cataataact
     4201 ttattaataa atcttttaag ttattttact ttaattacaa caaaagtcac atcatcatcg
     4261 ggttctttat cattagtcca tgatcttgct tctgttctta aatctgaaat tatttcttcg
     4321 ggttctttct ttgctgattc ttcaaaagag tttcttgctc ttttgtatcc atacatttcc
     4381 tgattctcat tctttaattc agcaaaccca tcactcatta ataataaagt atcgcctctt
     4441 tcaatcttaa attccttaat atcatatgca tttcctttca ttgcgccgag aggcatgtta
     4501 tttattacaa cttcctcaat tgtctgagaa tattttctgt aaataaacaa tggcggcata
     4561 ccggcatttg tagtgactac tttattgttg ctaatctgta cacaggtaag tgccatcata
     4621 agtctgccaa gctgcatatc ctttatcgct tcattcgcat tttcaaagaa gggcttaagc
     4681 tctttgttag ttctatcaga cataaaaagc gatttcataa ttgaaaccat cattcccgac
     4741 atcattccat gtccggttgc atctcccaaa acaattgtta gggtgccatc catatgaaca
     4801 tggaaatcat aataatcacc gccaacttca gttgcagttt tcataaatac tgcaatatca
     4861 agatggggca cgcttggcaa tgctttggga agcattgaaa gttgaagttg tctggcttct
     4921 tctaactctt ttgttttcct gtcattttca atctgcagga atttattatt ttcattttct
     4981 tttcttctct ctaattcata ttttcttact cctccgagca ttccaacaaa tcctaaaaag
     5041 taaagtccat atgcccacca tgttctccaa attggcggat tgactctgaa cgaatatgat
     5101 gcttcattgg ttttaactcc gttagctgta acaccccgca ctctaaatgt gtacttacca
     5161 aatgggagac tctgatattc tgcaaatggc agagtacttg ctttcgacca gcccttatcg
     5221 aaaccttcaa gaaagtgctc ataggttgtt tgcttgggat ctttataatt taaagcagca
     5281 taattgaaga taaatgaatt ttcagagtag ggtaattctt tcggtaaatt ataaaaataa
     5341 gatttaccag ttactgatac agacgaaatt aatagtgggt aacttttttc ttcactctta
     5401 ttaatgtaaa gcccaacccc attatagaat tctaacatat tcccgaatcc atccatagct
     5461 gaaatataag ggccgccgct cgcaacgctc tcatcgtgat aaaatgtctg aaatttatta
     5521 ccgcttattt ttaatgcacc tgaaggagat gaaaaataaa cagcttctct gatttcatcg
     5581 atgattggat ttgagagatt agtggataga agaccatctt ttttagtgta aaattttaga
     5641 tttgttccgt caaatattac cagtcccctg tttctaagtc ttatatataa atattcaccg
     5701 gcttgaattt ctgttgctgt tccagcgaca ctcgcgcctt ttaatattct aaaatctgca
     5761 ttctcattaa cgctatcact tagattgaca aaattatttc cattaaaaac ataaaatcct
     5821 ccaacggtta aaaagtacat tccatttttt aagttgccca catagtgtaa ttgttctgaa
     5881 ggaacattcc agcttgatgg cggctttgac caagatttac catcaaacaa cattattttt
     5941 gactgtacag atgtaatatc cttaatattg tctgagaata taccgaatag ccatagtctt
     6001 ccttcattat cttgcttaat atcaaaaagt tgatattttt tagcattatc agtttcatca
     6061 aaaggtgatt ttatagtaat aagtttgtct ccttctaatc ttccaaaata aggatcagct
     6121 cctataagat tatccgaagc aggtcttata tatttttgcc aggtataaat atcaccattt
     6181 tttgtttcca gaacatttga agtactataa ggtttaccat cttcatttgg aattgaatta
     6241 atcttaaata tttgttcgcc ttcgctgttt ctgatttcac gaatcagcgt taacccttta
     6301 gatgttgtaa tccaaactct ctttttgcta tcctgaaacg ttttgaaggt tatattactt
     6361 gccagtccat ctttttctgt aattgttcgt aaatattttc catcccaaac attaattcca
     6421 acatcaacgg ttaaagcata agcaggtaaa tccgtatatg aatatgaaaa ccagactagc
     6481 ccgctatagt ctgtataagg agttactata cccgattgtg taaccagcag accatctttg
     6541 gaagttaaca tacttgaata atttggataa taaattccga tgccttttga ggttttcatt
     6601 gctacacctt cacttagtgc aactgcaggg ccggattcaa atgaatcaaa ttttttatat
     6661 ttattatttt caaatgagaa tagttgatta agatattttg aactttgatt cagggcagta
     6721 acttgaaata caagtcgctc cggaaagtat ttacttgatt tcataaagaa aatactctga
     6781 tatttaaaat catttattac tctatctgtc ttcagatccc tgattgcatt aatttcctgc
     6841 aagatatttg aaaccttcca tttgccattt tcaaattcaa aaacactttt attctttcca
     6901 ttaaaaatct caccgcctct aattaaagta gtcataaaca acttattcat ttttgaac
//
LOCUS       DASWBY010000816         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_108892, whole genome shotgun sequence.
ACCESSION   DASWBY010000816 DASWBY010000000
VERSION     DASWBY010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108892"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VF870_14780"
     CDS             <1..749
                     /locus_tag="VF870_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9253507.1"
                     /translation="LVQYGPIWENKSESKEKIKNLFDQINGLDLVIFPEMTLTGFTMK
                     SKEFAEDLNGESYQFFSSLAKQKKSALIYGVIEKGRKKNFNTLVHLNNQGKILNSYRK
                     IHPFSYSQEDEFYGKGKETVTTKVKGFKIGLSICYDLRFPELYRFYAKEKVDMIIDIA
                     NWPDTRIEHWRALLKARAIENQCYAVGVNRVGDDPKLHYNGFSSVFDPMGKEIIAVEN
                     EEKIIAAEIDKAYVDEVKNRLPFLSDIRLI"
     gene            839..1237
                     /locus_tag="VF870_14785"
     CDS             839..1237
                     /locus_tag="VF870_14785"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9253508.1"
                     /translation="MFTLKRKINFFDCDPAGIIFYSRIFEFCHSAYEQMISSFNLKEN
                     YWMNEDYVVPIIHSESEYVKPIKYGDEITVNVQVSQLKNSSFELTYVIKRENTLCCHA
                     KTVHVFVDKKSWKKKEMYKDLVEGLNLHKD"
     gene            complement(1234..>1599)
                     /locus_tag="VF870_14790"
     CDS             complement(1234..>1599)
                     /locus_tag="VF870_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminobenzoate-CoA ligase"
                     /protein_id="HEX9253509.1"
                     /translation="FYHTGDLGFVDDDGYLFIEARRNDLIVTGGENVNPLEVEKALLK
                     IPFVKDACVFPKQNKTWGQIVASAIVCYDSSVDGKFLREILKQNLAGYKIPKEFYFVD
                     KLPKTSLGKLERNKIRKMF"
BASE COUNT          589 a          216 c          269 g          525 t
ORIGIN      
        1 gtttggttca atatggtcca atttgggaaa ataaatctga gagcaaagag aaaataaaaa
       61 atttatttga tcagattaac ggattggatt tagtaatttt tcctgagatg actttaactg
      121 gttttacaat gaaatccaaa gagtttgcag aggatttaaa cggagagagt tatcagttct
      181 tttcttcact tgccaaacaa aaaaaatctg ctttgattta cggcgtaatt gaaaagggaa
      241 ggaagaaaaa ctttaacact ttagttcact taaacaacca gggaaagatt ttaaattcat
      301 acagaaagat tcatcctttc tcttactctc aggaagatga attttatggt aaaggtaaag
      361 agactgtaac tactaaagtt aaaggattta aaataggact ttcaatttgt tatgatttac
      421 gttttcctga attgtataga ttttatgcta aagaaaaagt tgatatgatt attgatattg
      481 caaactggcc cgatacaaga attgaacatt ggcgggcatt acttaaagca cgggcaattg
      541 aaaaccaatg ttatgcagtc ggagtaaata gagtcggcga cgatcctaag cttcattaca
      601 acggttttag ttctgttttt gatccgatgg gaaaagaaat tattgctgtt gaaaatgaag
      661 aaaaaattat agctgcagaa atagataaag cttatgttga tgaagtgaaa aacagattac
      721 catttctgag cgatataaga ttgatttaaa ttttcaaaac tatttatagc tgttaaaaaa
      781 acaataattg tttaaataaa agataataga aaaaaagaag ggttgatagc ttatcaatat
      841 gtttacatta aaaagaaaaa taaatttctt tgattgcgat ccggctggaa taatttttta
      901 ttccaggata tttgaattct gtcactcagc ttatgagcag atgatttcca gttttaatct
      961 taaagaaaat tactggatga atgaagatta tgttgttcct attatccatt cagaaagcga
     1021 atatgtaaag ccgataaaat atggagatga gattacagtt aatgttcagg tatcccaatt
     1081 aaaaaattct tcatttgagt tgacatatgt aattaagaga gaaaatacac tttgctgtca
     1141 cgcaaaaacg gttcatgttt ttgttgataa aaaaagctgg aagaaaaaag aaatgtacaa
     1201 agacttagtt gaaggactga acctgcacaa ggactaaaac atttttctga ttttattgcg
     1261 ttctaatttt cccaaacttg tttttggtaa tttatccaca aaataaaatt cttttggaat
     1321 tttatatccg gcaagatttt gctttaatat ttctcttaaa aattttccat ctacagatga
     1381 atcataacaa acaattgcag atgcaactat ttgtccccag gttttatttt gtttagggaa
     1441 aacgcaagca tcttttacaa atggaatttt aagtagtgct ttttcaactt caagaggatt
     1501 tacattttca ccaccggtaa caatcaaatc atttcttctt gcttcaataa ataaatatcc
     1561 atcatcatca acaaaaccca aatctccggt atgataaaa
//
LOCUS       DASWBY010000817         4403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_148097, whole genome shotgun sequence.
ACCESSION   DASWBY010000817 DASWBY010000000
VERSION     DASWBY010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148097"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VF870_14795"
     CDS             <1..526
                     /locus_tag="VF870_14795"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9253510.1"
                     /translation="KIYRGFNFVAGEIGHMMVQKNGPKCGCGKKGCFEALASRTAIVN
                     NIIRDVKRSKKKSVLSDLIKSNQRVKSGALKNALDKKDKVVTKRITDACEIIGDVLGS
                     IANFMNFDMIVLGGGVIEALEDFIMPIIKNEFNNHVLDASAEGIKIVSSKLGDDAAIY
                     GGLALAEEFLGIKI"
     gene            complement(575..2524)
                     /locus_tag="VF870_14800"
     CDS             complement(575..2524)
                     /locus_tag="VF870_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX9253511.1"
                     /translation="MIDLSNVSLQFGGKYLFKDVNYKISSGDKISLVGANGTGKSSLL
                     KIISGSLQPESGEILKQKRISIGYLPQDHVTHIGKTLLEEASSALTDIIELQKKETSL
                     AEALANPNLTEEEQMDLAHQLGEVHHKLDGLDSYSAESKVEKILIGLGFAEEDFNRLT
                     DEFSGGWQMRIALAKILISQNDILLLDEPTNHLDIDSLEWLIDFLKDYKGGLLIVSHD
                     KNFINQVTNRTLEIFLGKFYTFKGDYDSYIKYKTERDELTVHQYEQQQKKIKETQKFI
                     ERFRYKATKSRQVQSRIKQLDKVDLIELPEDKSEINIRFSEPPQSGRTPMKLVSIFKS
                     YGDKKVFEGIDFEIEKGEKIAFVGPNGAGKSTLAKIIAGVIDYNSGQRTLGHNTIISY
                     YAQDVADNLNPNLDIIETVDGIDENKTVGQLRSLLGSFLFSGDDVFKKVGVLSGGEKS
                     RIALCKILLTKANFIILDEPTNHLDYTSKLILQKALIDFSGSLILVSHDVDFLRPIAS
                     KIVDIRKGKLKTYLGDIDYFLSKRELSSLERDAVAVEKKEKVEGVNRKEQKRLEAELR
                     QQKHLATKELTKEISRWEIKISSYENLVKDLENKLADPSIYSDGEAAKDITNRFNKTK
                     SELELANKKWEELTEKLLKIESKFA"
     gene            complement(2534..>4403)
                     /locus_tag="VF870_14805"
     CDS             complement(2534..>4403)
                     /locus_tag="VF870_14805"
                     /inference="COORDINATES: protein motif:HMM:NF044821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX9253512.1"
                     /translation="NDITISGDTVYVATDFGISSIDSKNYIFYDTYFKFGSFTSNIKV
                     NNVLFSDIIYAATESGVAIQKAGATNLSAPESWNVYDQSSGLPTGGTNVLSFYNSTLI
                     AGTNSGLYSFDGSTWQPFISLAGNKILDLLVTSNSLNILTPTQVFRYDETNLSELWIT
                     YSQPVKLSYQENAGLITATKLGIYVNNNFVYPNGPAANQFPNMTVDKEGDLWSASGKD
                     VTGAGIYKYDGSEWETYNVENYPDLYTNAFYSIFSSSDNSIYGGSWGQGFVKINGDKI
                     LRYHTGNTEMVYTRNTANNTGFIVISGFAEDSKNNMWIINLNAADGKSLYMLTPDSTW
                     YSFINPLEQHLTFSEVKDLVIDQYGTKWYTMSNEGSLGLFYFNEKGTYTDPSDDVYGY
                     LTTSKGLSSNAIYSLAVDRRGDLWVGTSLGVNIITNLNTVLSSSNPQLRITNSFSVRQ
                     QTVNALAVDPLNQKWLGTNQGLFLLSTDGTQLLATLDTKNSPLLSDQIESIAIDEKTG
                     RVYVGSASGLTSFDTPSILPVESFNGLTIYPNPLVITDGSKLVTIDGLIRNTDIKIVT
                     VSGKLVREFSSPGGRTAFWDGRDDNGNLVSSGVYIVIAFDQEGNNVETGKIAVLRE"
BASE COUNT         1383 a          776 c          737 g         1507 t
ORIGIN      
        1 gaagatttat cgtggtttta attttgttgc cggtgaaata ggacatatga tggtccaaaa
       61 aaacggtcca aaatgcggct gcggaaagaa aggctgcttt gaagcacttg caagcaggac
      121 agcaattgta aataacatca ttagagatgt taaaagatca aaaaagaaaa gtgtactttc
      181 agatttaatt aaatctaatc aaagagttaa aagcggagct ttaaagaatg ctcttgataa
      241 aaaagataaa gttgttacaa aaagaattac cgacgcttgt gaaatcattg gagacgtact
      301 tggcagtatc gctaacttta tgaattttga tatgattgtg cttggaggag gagttattga
      361 agctcttgag gattttataa tgcccataat caaaaatgaa tttaataatc atgttctgga
      421 tgcatctgca gaaggaatta aaatagtatc ttcaaaactt ggcgatgatg ctgcaattta
      481 tggcggttta gctttggctg aagaattttt aggtattaaa atttaatgaa gtctttgcga
      541 gctttgctta tattttttag catctgattt tttatcaagc aaatttagat tcaatcttta
      601 gaagcttttc agttagttct tcccattttt tattagccaa ctcaagttca gatttagttt
      661 tattaaatct gttagtaata tcttttgcag cttcaccatc gctgtaaatt gatggatcgg
      721 ctaatttatt ttcaagatct ttaaccagat tttcataaga acttattttt atttcccaac
      781 gtgaaatttc tttcgttaat tctttagtag caagatgctt ttgctgccgt aattcggctt
      841 caagtctttt ttgttcttta cgattgacac cttccacttt ttcttttttc tcaaccgcca
      901 cagcatctct ttcaagtgat gataactccc tttttgaaag gaagtaatca atatcaccaa
      961 gatatgtttt taatttgcct tttcggatat caactatttt agatgcgatt ggtctcagga
     1021 agtcaacatc gtgagaaaca agaataagtg aaccactaaa atcgattaat gctttttgta
     1081 aaatcaattt tgaagtataa tcgagatggt tagttggttc atccagaatt ataaaattag
     1141 cttttgtaag taaaatttta caaagtgcta ttctgctttt ttctccacct gaaaggaccc
     1201 caactttttt aaatacatca tcaccggaaa aaagaaatga acccagtaat gaacgcagct
     1261 gtccgactgt tttattctca tcaattccat caacagtttc aataatgtct aaattcggat
     1321 taagattatc agcgacatcc tgtgcataat aagagattat tgtgttatgc cctaaagttc
     1381 tttggccgga attataatca attactccgg caataatttt tgcaagcgtt gatttaccag
     1441 ctccatttgg accaacaaac gcaatctttt cacctttttc tatttcaaaa tcaattccct
     1501 caaaaacttt tttatcgccg taagatttaa agatagaaac aagtttcatt ggcgttcttc
     1561 cgctttgcgg cggttctgaa aatctgatat taatttcact cttatcttca ggcaactcga
     1621 taagatcaac tttatcaagc tgcttaattc tgctttgaac ctgtctggat tttgtcgctt
     1681 tgtacctgaa ccgttcgata aacttttgag tttctttaat tttcttttgt tgttgttcgt
     1741 attgatgaac tgttaattcg tcccgctccg ttttatactt gatgtaggaa tcataatcac
     1801 ctttaaacgt ataaaactta ccaagaaaaa tttccagtgt acgattagta acctggttaa
     1861 taaagttttt atcgtgagag acaattagca aaccgccttt gtaatcttta agaaaatcta
     1921 taagccactc aagtgaatca atatcaaggt gatttgtagg ttcatccaaa agtaaaatgt
     1981 cattttgcga gataagaatt tttgcaagag caatacgcat ctgccagccg ccggaaaatt
     2041 catctgttaa tctgttgaaa tcttcttctg cgaaaccaag tccgattaat attttctcaa
     2101 ctttagattc tgcggaataa gaatctaatc catccaattt atgatgaact tcgccaagct
     2161 gatgtgcaag atccatttgt tcttcttcgg taagatttgg atttgctaac gcttcggcta
     2221 atgatgtttc ctttttttgc agttcaatta tatctgttaa tgctgatgac gcttcttcta
     2281 aaagagtttt accaatgtgg gtaacatgat cctgcggaag atatccaatt gaaattcgtt
     2341 tttgctttaa aatctcaccg gattctggct gaagagatcc ggagataatt tttagaaggg
     2401 aggatttgcc agtgccatta gcaccaacta aagaaatttt atcgccggaa gaaattttgt
     2461 aattaacatc tttaaaaaga tatttgccac cgaactgaag tgaaacattt gaaagatcaa
     2521 tcatattcta aaactattct cttaatactg caattttacc ggtttcaaca ttgttgcctt
     2581 cctgatcaaa agcgataacg atgtaaactc cgctgctgac taaattacca ttatcgtctc
     2641 gtccatccca aaaagcggtg cgcccaccgg gagatgagaa ttcgcgaact aacttgccgg
     2701 agacagtcac aattttaata tcagtatttc tgataagccc atcaattgtt actaatttac
     2761 ttccatcagt aatcacaagc ggatttggat aaatagttaa accattaaaa ctttcaactg
     2821 gcagaattga tggagtatcg aatgaagtta aaccagatgc actgcctaca taaactcttc
     2881 cggttttttc atcaattgca atgctttcta tttgatctga aagcagcgga ctattttttg
     2941 tatcaagtgt agcaagtaat tgagttccat ctgtgcttaa taaaaacagt ccctgatttg
     3001 tgccaagcca tttttgattt agcgggtcaa ctgcaagagc atttaccgtt tgttgtctta
     3061 cggaaaatga atttgttatt ctaagctgtg gatttgagga acttaaaaca gtattaagat
     3121 ttgttattat gttaactcct aagctcgttc caacccaaag atcacctcgg cgatcaacag
     3181 ctaaactgta tattgcatta ctcgataaac ctttactggt tgtcagatat ccatatacgt
     3241 catcagatgg gtcagtgtat gttccttttt cattaaaata aaataagcct aaactgcctt
     3301 cattactcat tgtgtaccat ttagtgccat actgatcaat aactaaatct ttcacttcgc
     3361 taaaagttaa atgctgttca agaggattaa tgaatgagta ccaagtacta tctggtgtta
     3421 acatatataa tgatttacca tcggcagcat ttaaattgat aatccacata ttgttttttg
     3481 aatcttctgc aaatccgctt ataactatga aacctgtatt gtttgcggta ttacgggtgt
     3541 ataccatttc tgtattacca gtatgatatc ttaaaatttt atcaccatta attttaacaa
     3601 aaccttgtcc ccagcttcca ccataaatag agttatcaga agaggaaaaa attgagtaaa
     3661 aagcattggt atataagtcg ggataatttt caacattata tgtttcccat tcagagccat
     3721 catatttgta aatcccagca ccagtaacat ctttaccgct cgctgaccat agatcacctt
     3781 ctttatcaac agtcatattt ggaaattgat ttgcagcagg accatttgga taaacaaaat
     3841 tattgttaac atagattcct aatttagtag cggttattaa tcctgcgttt tcctgatagc
     3901 taagtttgac tggctgcgaa taagtgatcc ataattcaga taaattagtt tcatcatatc
     3961 taaaaacttg agtgggtgta agaatattta aagaattact tgttacaaga aggtctaata
     4021 ttttatttcc cgctaagctt ataaatggct gccatgtgct tccatcaaaa gaatataagc
     4081 cggagtttgt tcccgcaatt aatgtgctgt tgtagaatga taaaacgtta gttccaccag
     4141 ttggaagtcc gcttgattga tcataaacat tccaggactc aggagcagat agattagtag
     4201 cgcctgcttt ttgtatcgcg acaccggatt cagttgccgc ataaattata tcagaaaata
     4261 aaacgttatt tacttttatg ttagaagtaa aacttccaaa tttaaaataa gtatcataaa
     4321 atatataatt ttttgaatcg attgaggaaa ttccaaagtc agttgcaacg tagacagtat
     4381 ctccagaaat agtaatatca ttt
//
LOCUS       DASWBY010000818         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_296311, whole genome shotgun sequence.
ACCESSION   DASWBY010000818 DASWBY010000000
VERSION     DASWBY010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296311"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VF870_14810"
     CDS             complement(<1..599)
                     /locus_tag="VF870_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253513.1"
                     /translation="MKLSKEERTVLKTGKSKFKTILLLVLLSEIISPQIPFKGFCKLN
                     SFEIDSGFTKLFSINYDNNEHSDLLVFNPAQKTAKIYFGSAGIKFSSNKTISFPLEVS
                     VIEPIILPSNMIESFAFTSRKNKSFGIYKFSTTGNPSLSAQIKFNSYPENISIVNNPD
                     NSKSFLISGNSFDGLSFISYRNNTLKEIKVSQKHIFQNA"
     gene            complement(550..1020)
                     /locus_tag="VF870_14815"
     CDS             complement(550..1020)
                     /locus_tag="VF870_14815"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9253514.1"
                     /translation="MPAAKKISDSQITMTELVLPNHTNQLGNLLGGQLMHWIDICAAL
                     SAAKHNQRVCVTASVDKIDFHHPIRLGDAVTLTSTINRVFKTSMEVGVKVYAQNFREG
                     TKIHTNSAYLTFVSVDNNGKPVEGIEGIPVTIDEKRRYDEALQRRENRLKDRKK"
     gene            complement(1020..1778)
                     /gene="bamD"
                     /locus_tag="VF870_14820"
     CDS             complement(1020..1778)
                     /gene="bamD"
                     /locus_tag="VF870_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX9253515.1"
                     /translation="MKNIFALIFVTLFLFACSSTLDTTNMAPEERLAYAIKLYQEEDS
                     EEAVKEFESIILQYPGSSIIDDAQYYLAMTRFQREEFILAAYQFSKLIKGMPSSEFLA
                     DAQYMLAECYYQLSPDFTLDQQYTKKAIEEYQVFIDFFPLNPKVADAEVKITELNDKL
                     ARKEFDAARIYEKMEYYNAAFKYYDSVMEIYHDTQYAPLALYKKINLLVERERESEAL
                     TEAQKFLQKYPQDENFGAVEKIKNSLESKLSVNQ"
     gene            complement(1848..2411)
                     /locus_tag="VF870_14825"
     CDS             complement(1848..2411)
                     /locus_tag="VF870_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253516.1"
                     /translation="MLLLQISVYSQTISNLDRFYTLIDSASSLLLKDLGDVKKVNLEL
                     NFGVDYSVFANQVRGKLLRNGVQLLSDNSEDKDFVRVNFVVDNSFVGYSEPEKDGIFG
                     DFFTERTIELSGNYFISNQSQVKDFNLSILDTINVEDVEYVENRSYPFTRGDLPAEPF
                     FSSLLEPIVAIGAAATVVILFFSVRSK"
BASE COUNT          799 a          419 c          399 g          870 t
ORIGIN      
        1 tgagcgtttt gaaaaatatg tttctgtgat actttaattt cctttagagt gttattccga
       61 taagatataa atgataagcc atcaaaagaa tttccggata tcaaaaatga ttttgagtta
      121 tctgggttat ttacaatact tatgttttca ggatatgaat taaattttat ttgagcagat
      181 aaagatggat tgcctgtagt actgaattta tatattccaa aacttttgtt ttttctggat
      241 gtaaatgcaa aagattcaat catattgctg ggaaggatga tcggttcaat tacggaaact
      301 tccagaggga atgaaatggt tttgttagag ctaaatttta ttccggcact tccaaaataa
      361 atctttgctg ttttttgtgc tggattaaaa actaataaat cagaatgctc attattatca
      421 taattaatac taaataactt tgtaaaacct gaatcaattt caaatgaatt taatttgcag
      481 aatcctttaa aaggtatttg cggagaaatt atttctgaga gcagaacgag tagtaaaatt
      541 gttttgaatt tactttttcc tgtctttaag acggttctct cttctttgga gagcttcatc
      601 atatcttctt ttttcgtcta ttgttacagg aattccctca ataccttcaa ctggcttgcc
      661 gttattatca acactaacaa atgtaaggta tgcggagttt gtatgaatct ttgtgccttc
      721 tctaaaattt tgcgcgtaaa cttttactcc aacttccatc gaggttttaa aaactctgtt
      781 gatagtggag gttaaagtaa cggcatcacc taatcttata ggatggtgaa aatcaatttt
      841 atccaccgaa gctgttacac aaactctttg attatgtttt gcagcactta atgcagcaca
      901 aatatcaatc cagtgcatca actgtccgcc caacaaatta ccaagctgat tagtatgatt
      961 tggcagaacc aattcagtca ttgtaatctg agaatcgctt attttttttg cagcgggcat
     1021 tattggttta ccgagagctt gctttctaat gaattcttaa ttttctcaac tgcaccaaag
     1081 ttttcatcct ggggatattt ctgcagaaat ttttgtgctt cagttaatgc ttctgattcc
     1141 cgttctcttt caactaataa atttattttt ttgtaaagag caagaggagc gtattgagtg
     1201 tcgtgataaa tttccattac agagtcataa tatttaaagg ctgcattgta atactccatt
     1261 ttttcataaa tgcgtgcagc atcaaattct tttcttgcca gtttatcatt tagttctgtt
     1321 attttgactt cggcatctgc tactttcgga tttagcggaa agaaatcaat aaacacttga
     1381 tactcctcaa ttgctttttt agtgtattgc tgatccaatg taaaatccgg tgatagctga
     1441 tagtaacact cagctagcat atattgtgca tctgcaagaa actcgcttga aggcattcct
     1501 ttaatcaatt tgctgaactg ataagctgca agtataaatt cttctctctg gaatcttgtc
     1561 atcgccagat aatattgtgc atcatcaata atgctgctcc cgggatattg aagaattatt
     1621 gattcaaatt cttttaccgc ttcctcggag tcttcttcct gataaagttt tattgcataa
     1681 gcaagtcttt cttcaggagc catattggtt gtatctaaag ttgaagaaca agcaaataaa
     1741 aataatgtta caaatattag cgcaaatatg tttttcatct taaagtttat aggttttttg
     1801 atgtgcaatt ataaccaaat tagcaaagcc ttcaaaggta tttatgatta cttagaacgc
     1861 acagaaaaga atagaatcac aacagtggca gcggctccaa ttgcaactat tggttctaaa
     1921 agactcgaaa agaaaggttc agctggaagg tcccctctcg taaatggata tgatctgttt
     1981 tccacgtatt caacatcttc aacatttatt gtatccagga tagaaagatt aaaatctttg
     2041 acctgagatt gatttgaaat aaaataattc cccgatagct ctatagttct ttcggtaaaa
     2101 aaatctccaa aaattccatc tttttcgggt tcagaatatc caacaaaaga attatcgact
     2161 acaaaattaa ctctgacaaa atccttatct tcagaattat cgcttaacaa ctgaacgccg
     2221 tttcttaaaa gctttcctct aacctgattt gcaaataccg aataatcaac tccaaaattt
     2281 aattccaggt taactttttt tacgtcccct aaatctttta acaataaaga agatgctgaa
     2341 tcaattaaag tataaaatcg atcgagatta gaaatggttt gtgaataaac agagatttgt
     2401 aaaagaagca aaaaaaataa aaaccggaga gattttttta tcactccggt tattttaaat
     2461 aaatcgtaaa tcatcttgag tcaaata
//
LOCUS       DASWBY010000819         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_369751, whole genome shotgun sequence.
ACCESSION   DASWBY010000819 DASWBY010000000
VERSION     DASWBY010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369751"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1329
                     /locus_tag="VF870_14830"
     CDS             160..1329
                     /locus_tag="VF870_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9253517.1"
                     /translation="MSKFIGVDYFNIDSLLSEEELMIRNTVREFVSEEVIPIIEEYNR
                     ESKFPHQIIPKMAEMGLFGPTLPAKYDCAELNNVSYGLIMQELERGDSGIRSFASVQS
                     ALVMYPIFTFGSEEQKSKWLSELAKGEKIGCFGLTEPDFGSNPGGLITKAEKADGGYI
                     LNGAKMWITNGTIADVAVVWAKLDGIVRGFLVEKGMKGFTAPEMKGKHSLRASITSEL
                     IFDNVFVPDENLLPKTEGLKNALMCLNQARYGIAWGVVGSMMACYDSALNYAKSRKQF
                     SKPIAGYQMTQSKLVYMVTEITKAQLLNLQLGRLKDKGTLKFTQVSMAKRNNCEIALD
                     IARMSRQILGANGILDEYPVMRHAANLESVITYEGTHEMHTLIIGEDLTGYQAFD"
     gene            1366..>2303
                     /locus_tag="VF870_14835"
     CDS             1366..>2303
                     /locus_tag="VF870_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9253518.1"
                     /translation="MIDKKFLEMGLTFDDVLLVPGKSAVLPREVDITTYLTPEIKLNI
                     PIISAAMDTVTESSMAIALAREGGIGILHKNLSIEDQCLEVDKVKRSESGMIRNPITL
                     TPDKTIGDALEIMKKYRVSGIPVIDKNNLLVGILTNRDLRFEPNKKLKVSDLMTKENL
                     ITAPIGTTLEKAEVLLQRYKIEKLPVVDKSGKLKGLITFKDILKKKKHPFACKDEQGR
                     LRVGAAVGVTYDSIDRIKALTDAGADAIIIDTAHGHSQGVLKTVKAVRKKFKYIQLIV
                     GNIATYEAALDLINCKVDAIKVGIGPGSICTTRVIAG"
BASE COUNT          784 a          338 c          481 g          700 t
ORIGIN      
        1 taaatatatc agatccaaaa ttaatttcca ttaactagtg attttttagt tgcaatctca
       61 ttcattatat aatcaaaaca ttatcataat tttgaaatta aaattgtttc tctaatcacc
      121 ttttaataat tttagccatt caaattttag aaagatacaa tgagtaagtt cataggagta
      181 gactatttta acatagattc cctgctttca gaggaagagt tgatgattag gaataccgta
      241 cgtgaatttg ttagtgaaga agtaattcct atcattgaag aatacaatcg cgaatctaaa
      301 tttccgcatc agattattcc taaaatggca gaaatgggtc tgtttggtcc gactcttcct
      361 gcaaaatatg actgtgcaga attaaacaat gtttcctatg gattgattat gcaggagttg
      421 gaaagaggtg atagcggaat cagaagtttt gcctcggtcc agagtgcatt agttatgtat
      481 ccaatcttta cttttggaag tgaagaacaa aaaagtaaat ggttatcaga attagcaaaa
      541 ggggagaaga taggctgctt tggtcttacc gaacctgatt tcggatcgaa tcccggtgga
      601 ctgattacga aggcagaaaa ggctgatggt ggttacatcc ttaatggtgc taagatgtgg
      661 attacaaatg gtaccattgc ggatgttgcg gttgtatggg caaaattaga tggtattgtt
      721 cgcgggtttc tagtcgaaaa gggaatgaaa ggttttacag ctcctgaaat gaaagggaag
      781 cattcactaa gagcgtctat tacctcagaa ttaatatttg ataatgtatt cgttccggat
      841 gaaaacttgc tcccgaaaac ggaaggcttg aagaatgctt taatgtgttt gaatcaagca
      901 agatatggaa ttgcatgggg agttgttggc tcaatgatgg cttgttatga ttcagctttg
      961 aattatgcca aatcaaggaa gcaattcagc aaacctatag ctggatatca aatgactcaa
     1021 agtaagttag tatatatggt tacagaaatt accaaagccc aattattaaa cttgcaatta
     1081 ggaagattaa aggataaagg aacattaaag ttcactcagg tttctatggc aaaaagaaat
     1141 aactgcgaga ttgctttaga tatagctaga atgtccagac agatattggg agcaaatggc
     1201 atattggatg aatatcctgt aatgcgtcat gctgctaatc ttgaatcagt tattacttat
     1261 gaaggcacac atgaaatgca tactctaatt attggtgagg acttaacagg ttatcaggcg
     1321 tttgattaat tttttctatt ttacttgtga acgaaaagga aatttatgat tgataaaaag
     1381 tttttagaaa tgggtttaac atttgatgat gttttactgg ttcctggtaa atctgctgtt
     1441 ctccctcgtg aagtagatat aacaacatat ctaactcctg aaataaaatt gaacattcca
     1501 ataatttctg cagcgatgga tacggtaaca gaatcctcaa tggctattgc tctggcaaga
     1561 gaaggcggca ttggtatatt acataaaaac cttagcattg aagatcagtg ccttgaagtt
     1621 gataaagtga agcgttctga aagcgggatg ataagaaatc caattactct cacacctgat
     1681 aaaactatcg gtgatgctct tgaaatcatg aaaaagtata gggtttcagg tattcccgtt
     1741 atagataaaa ataatttgct tgtaggtatt cttacaaaca gagatttacg ctttgaacca
     1801 aacaagaaac ttaaagtttc ggatttaatg actaaagaaa atctgattac tgcacctatt
     1861 ggtacaacct tggaaaaggc tgaagtatta ttacagcgat ataagattga aaaattaccc
     1921 gtagtggata agtctggcaa attaaaagga ttgataacat ttaaagatat tcttaaaaag
     1981 aagaagcatc catttgcctg taaagatgag cagggaagat taagagtggg agcagcagtc
     2041 ggagtaacct atgattctat tgatagaata aaagcactaa ctgatgctgg ggcagatgcc
     2101 attattatcg atactgctca cggtcattct caaggcgttc ttaaaacagt aaaagcagtc
     2161 aggaaaaaat tcaaatatat tcagttaatt gttggtaata tagctactta tgaggccgca
     2221 cttgatctta taaattgtaa ggtagatgcg atcaaagtcg gcatcggacc tggatcaatt
     2281 tgtactactc gagtgattgc agg
//
LOCUS       DASWBY010000820         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_598472, whole genome shotgun sequence.
ACCESSION   DASWBY010000820 DASWBY010000000
VERSION     DASWBY010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598472"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VF870_14840"
     CDS             complement(<1..1169)
                     /locus_tag="VF870_14840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9253519.1"
                     /translation="MINKTILHYNILSKIGEGGMGIVYKAEDTKLKREVAIKFLPQSI
                     TVSENDKEKLKTEAQIAASLNHPNIATVYAIEEYGDDTFIVMEYVIGNELKDVIRNSL
                     DKKLNLNHFLNYAIQITEGLNAAHKKGIIHRDIKTSNIMLSEDGRIRVMDFGLAHIHG
                     DPLVTKKGSTPGTTAYMSPEQIRGEEIDFRADIWSLGIVLFEMLTGQPPFQGNFDQAI
                     IYSILHEKPKSPKKLNPNTPDELEQTILSCLEKDRNKRIKSAEELLTQLKTIQSQASF
                     STTPKFSPSRLKIGKAGLIASTIIVAILIIIAVFIPLKKIFNPINVSELAQQLNDLVI
                     SEKYFAAYDLSKEYQDELKNNATFNKLSLIIYDTLSVITEPEGAKVYLKRFDPAN"
BASE COUNT          500 a          263 c          266 g          580 t
ORIGIN      
        1 tcattagcag gatcaaatcg ctttaaataa acttttgctc cttcgggctc agtgataaca
       61 gaaagtgtat cataaataat taagcttagc ttattgaagg tagcattatt tttcaattca
      121 tcctgatatt ctttagataa atcataggct gcaaaatatt tttctgatat tacaagatca
      181 ttaagctgtt gagctagttc tgaaacattt atgggattaa atattttttt aagcggaatg
      241 aaaacagcaa taataataag gatggccaca ataatagttg atgcaattaa tcctgcttta
      301 cctatcttta atctagaagg actgaattta ggagtagttg aaaaagatgc ttggctttga
      361 attgttttaa gttgtgttaa cagttcctct gcagatttaa ttctcttatt tcgatctttc
      421 tcaagacaag atagaattgt ctgttcaagt tcgtctggcg tattgggatt tagttttttt
      481 ggcgatttag gtttttcgtg aagtattgaa taaataatcg cctgatcaaa atttccctga
      541 aaaggtggtt gtcctgtaag catttcaaac aaaacaattc ctaatgacca aatgtcagca
      601 cgaaaatcaa tttcttcccc gcgtatttgc tcgggggaca tataagctgt tgtgccggga
      661 gtagaacctt tctttgttac caaaggatca ccgtgaatat gggctaagcc aaaatccatt
      721 actcttattc tgccatcctc tgacagcata atgttgctag tctttatatc acgatgaata
      781 attccttttt tatgtgcagc gttcagtcct tcagtaatct gaattgcata gttcaagaag
      841 tgattaagat ttagtttttt atcgaggcta tttcggataa catccttaag ctcattgcct
      901 atcacatatt ccataacgat gaaagtatca tcaccgtatt cttcaatcgc ataaactgta
      961 gctatgttcg gatgatttaa actggcagca atttgggctt cagtttttaa tttttcttta
     1021 tcattttcag aaacagtaat actttgcggg aggaatttaa tagcaacttc gcgctttagc
     1081 ttcgtatcct ctgctttata gacgatcccc atcccgcctt cgccgatttt agaaaggata
     1141 ttataatgta atattgtttt attaatcatt tacaactaaa aaatctatta cgttcaaata
     1201 tttattatta ggaaaataaa gaattgattg aaattatttt agttaaaata tcttgatttt
     1261 acaatcatta aaattaaaac ttgttataaa tcaaaaatac tttagggtat tcatttagct
     1321 tcaattaacg attgggaaaa ttcaagtaca gttttctaaa cgtttcgttt ctcgttcggg
     1381 gtgtgcaaaa aaacattaaa aaagccctga aatcagggct ttttaggtgg ctccgcaaac
     1441 ttaagtcact tagaattttc ttttccggca ataaatagct atttacctat caatattgtg
     1501 ttaaaatgtt ggctgggaag tgattttccc tgctttccat ttgcttactt cagcagcaac
     1561 atctttttag tcttaacaaa aatttgagat tgcaatttgt aaaaataaa
//
LOCUS       DASWBY010000821         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_749002, whole genome shotgun sequence.
ACCESSION   DASWBY010000821 DASWBY010000000
VERSION     DASWBY010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749002"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VF870_14845"
     CDS             complement(<1..553)
                     /locus_tag="VF870_14845"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HEX9253520.1"
                     /translation="MDSKKYNNTKLVIGIAKAVMSFILLFLFIILGYSLSLQNYIQTI
                     TENKYLSFLIFVFVIGIFSSVLFMPVNFYTGFYLEHKYKLSNQTFIKYIIENLKSMLV
                     GLVIGIPILLLFYYVLNQFGDLWWLVFASAMFLISVVLSQLFPILILPIFYKILPLDD
                     EELKTRIRNLAKGAGIKVENVFTF"
     gene            664..1407
                     /locus_tag="VF870_14850"
     CDS             664..1407
                     /locus_tag="VF870_14850"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9253521.1"
                     /translation="MKIIHISFLTAALCVIICTYQTNAQVTLQIGAGLGYSTPTGDYG
                     GNTTEFYNGTKYGIESGFNYHAKARVGLLFMNVFGEIGYTTFSGEGEATPGQGNIKLS
                     NNVFSMKVGPEFPITIPLSPITPYLMAFVAVNTFSGQVEFQGVSSVPSGTYDLASATR
                     VGIGGGGGVTFSAGPLKLDLNIQYHLINFTGNEYKIENVTSHERLDNYTSLNDGADPA
                     YVNGSDSHFIKDDRGIGAMEFKVTAMFGL"
     gene            complement(1485..2231)
                     /locus_tag="VF870_14855"
     CDS             complement(1485..2231)
                     /locus_tag="VF870_14855"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX9253522.1"
                     /translation="MEWITDPQALIALLTLTVLEIVLGIDNIIFISILSGKLPKEQRK
                     KGRLLGLALAMVTRILLLFSISLIVKLKEPFFTVFDLGISGRDLILILGGLFLLAKST
                     MEIHSKLEGEEEHHDIKAKVSFAGVIIQIMLLDIVFSLDSVITAIGMANDLAVMIIAV
                     IIAVIFMMFFSGSISDFVENHPTIKILALSFLLLIGFTLVVEGLHQHIPKGYIYFAMA
                     FSVFVEMLNLKMRKKKTEPIRLRKEIPGER"
     gene            complement(2386..2721)
                     /locus_tag="VF870_14860"
     CDS             complement(2386..2721)
                     /locus_tag="VF870_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253523.1"
                     /translation="MKNLLIVIALVTVSAFVVNAQSKDCCSKEKNETTKVEKSTQTDQ
                     TMKTGDKVETTTTTASTLTDGKETVNKEVVVTKNTMNKKENCDNKSSCCDVKKDKKTE
                     KVEKEVEKK"
     gene            complement(2804..>3269)
                     /locus_tag="VF870_14865"
     CDS             complement(2804..>3269)
                     /locus_tag="VF870_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX9253524.1"
                     /translation="IAKLNIKDRVDELPQFFIFILWEGMLASFSVSGWTYFVYSVFIL
                     LLLCVIAYFFSRSSTQQRLSFFSGVGLSALLILLIILLTVKINKEYNIKNGIIVENVI
                     TVKSSPDNSSKDEFVVHEGLKVRLEDKFDDWVKIRLADGKIGWISEKSLGVI"
BASE COUNT         1141 a          537 c          501 g         1090 t
ORIGIN      
        1 caaaagtaaa aacattttct acttttattc ctgcaccttt tgcaaggttt ctaatccgag
       61 tttttaattc ttcatcatcc aaaggaagaa ttttatagaa gattggaaga ataagaattg
      121 ggaataattg agagagaaca acagaaatca aaaacattgc agaagcaaaa acaagccacc
      181 acaaatcacc aaactgattt agcacataat agaagagtaa aagaatagga attccaatta
      241 ctaaaccgac taacatagat tttagatttt caattatgta tttaataaat gtctggttgg
      301 atagtttgta tttatgctca agataaaatc cggtataaaa attaacaggc ataaaaagta
      361 ccgagctaaa aattccaatt acaaatacaa aaatcagaaa tgataaatat ttattttctg
      421 taattgtctg aatgtaattt tgcagactta aactatatcc cagaatgatg aaaagaaaaa
      481 gtaaaataaa actcataacc gctttggcaa tcccgattac aagcttagta ttattgtatt
      541 tttttgaatc catatggtta aaaatagtaa ctgaatctct tgcaatcaat ttgataaaca
      601 ctgttatatc tgataatttt ttttcatatg tttaggaggt aaacaattta ttgagaggaa
      661 cttatgaaaa taattcacat ttctttttta actgcggcat tatgcgttat aatttgtact
      721 tatcaaacaa atgcacaagt aacattacaa attggtgcag gcctcggata ctcaacacca
      781 accggtgatt atggcggtaa cacaacagaa ttttataatg gtacaaaata cggaatagaa
      841 tcaggtttta attatcatgc caaagctcgc gttggacttt tatttatgaa tgtatttggt
      901 gaaattggtt acacaacttt tagcggtgaa ggagaagcga caccaggaca aggaaacata
      961 aaactctcaa acaatgtgtt ttcgatgaaa gttggaccgg aatttccaat cacaattccg
     1021 ctctccccaa ttactccata tcttatggca tttgtggcag ttaatacttt ttccggtcaa
     1081 gttgaatttc aaggtgtttc gagtgtgcct agcgggacat atgatcttgc aagtgctact
     1141 agagtcggaa tcggaggagg cggaggagtt acctttagcg ctggaccgct aaaattagat
     1201 ttaaacatac aatatcatct aattaatttt accggtaatg agtataagat tgagaatgta
     1261 acttcacacg aaagattgga taattatact tctttgaatg atggagcaga tccggcttat
     1321 gttaatggta gtgattctca ctttattaaa gatgatcgtg gtataggagc aatggagttt
     1381 aaagtaactg caatgttcgg tttataatct attttattaa tattaaaaca aacccagttt
     1441 ctcaatgaaa ttgggtttgt gattttaaac caatttaaaa ttaactatct ctcgcccgga
     1501 atttcttttc tcaatctaat tggttcagtt ttcttttttc tcatctttaa attcagcatt
     1561 tcaacaaaaa cgctaaacgc cattgcaaag tatatatagc ctttaggaat atgctggtgc
     1621 aaaccttcta caactaaagt aaaaccgatt aacagcaaga agcttaatgc aagtattttt
     1681 attgttggat gattctcaac aaaatcacta attgaacctg aaaagaacat cataaatatt
     1741 actgctataa ttactgcaat tatcattact gctaaatcgt ttgccatacc gattgctgtt
     1801 ataactgaat cgagtgagaa aacaatatca agcagcatta tttggataat tactcctgca
     1861 aatgaaactt ttgctttaat atcgtgatgt tcttcttcgc cttctaattt agaatggatt
     1921 tccatagtgc ttttggccag taaaaataat ccgccaagaa taagaattaa atccctgccg
     1981 gagattccca aatcaaaaac cgtaaagaat ggttctttta gttttacaat tagagaaata
     2041 gaaaagagta ataatatcct ggtgaccatg gcaagagcta atccaagaag tctgcctttc
     2101 tttctttgtt cttttggaag tttaccggat agaattgaaa taaaaattat gttatcaatt
     2161 ccaagtacta tttcaagaac agtcaatgtt agtaaggcaa ttaatgcctg cggatcagta
     2221 atccattcca ttttttttcc ctagttaaat tttattgagt aaaattagag agcgctttgt
     2281 ttaaaatgaa attggaggga tttatatctg gcacaaaaaa agattcccgc ttccgcggga
     2341 ataacaaaca caacataaat tatttatact taaaaataaa tgagtttatt ttttctcgac
     2401 ttctttttca accttttcag ttttcttatc tttctttaca tcacagcagg aagatttatt
     2461 atcacaattc tcttttttat tcattgtgtt tttggtaact acaacttcct tattaacagt
     2521 ctctttgcca tcagttaaag ttgatgcagt agtagtggta gtttcaactt tatcaccggt
     2581 tttcattgtt tgatcagtct gtgtggattt ttctacttta gtagtttcat ttttttcttt
     2641 agaacagcaa tctttcgatt gagcattaac tacaaatgcg gatacagtta caagagcaat
     2701 aacaataaga agatttttca ttatttacct ttctattttt tatttggtac ctgtcaaagc
     2761 taaagaaaac acaataattt acaaatgcaa atagttgggt tttctaaata acaccgagac
     2821 ttttttcact aatccaccca atttttccat ctgcaagtct gattttaacc caatcatcaa
     2881 atttgtcttc taatctaacc ttcaatccct catgaactac aaattcatct tttgaagaat
     2941 tatccggcga ggattttact gtgataacat tttcaacgat tattccgttt ttaatattat
     3001 actctttatt aatctttacc gttaatagga taattagaag aattaacaaa gccgataatc
     3061 caacaccact aaaaaaagat aatctctgct gtgttgaaga tcttgaaaag aaataagcaa
     3121 taacacaaag aagcagtaaa ataaagacag agtaaacaaa gtaagtccat ccagaaacag
     3181 aaaatgatgc aagcatacct tcccaaagaa taaaaataaa aaattgcggc agctcatcta
     3241 ccctatcttt tatatttagt tttgcaatt
//
LOCUS       DASWBY010000822         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_598502, whole genome shotgun sequence.
ACCESSION   DASWBY010000822 DASWBY010000000
VERSION     DASWBY010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598502"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VF870_14870"
     CDS             complement(<1..566)
                     /locus_tag="VF870_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX9253525.1"
                     /translation="MLNHPLPRIDKDEKLRKLLLPYSRFKEGTIWIDEKAGHKIACLD
                     VSKSDEVKKIFGKSKVKLSVQDPPYNFIAFTEKQNDDFIKWCKRWITLNKEISDKNSS
                     FYFWLGADQSNHFEPFGGFIEMMKSTGLSSRSFITMRNQRGYGTQHNWMAIRQELLYY
                     TNGKPMFNIKSEYTDIPKVLKGYYKEVGG"
     gene            complement(535..1374)
                     /locus_tag="VF870_14875"
     CDS             complement(535..1374)
                     /locus_tag="VF870_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX9253526.1"
                     /translation="MSSINLLFVGDIIGQPGMDIVQTWLPGLIQKYKADFVVVNGENI
                     SDGKGCTDKEGPKLFNLGVNVITGGNHTWDKHQSQDYLKAEPRSLRPINYPKGTHGNG
                     YHIADTKKGKVAVISLQGRTFMTPIECPFRTMDWILTRIKQETKVVFVDFHAEATAEK
                     MAMGHYLDGKVSAMVGTHTHIQTSDERIFPNGTGYITDVGMTGPYDSVIGMKTSAAVN
                     RFLFATPQKYETAKDNVHLTGMFFKIDAETGKTLELERIFFPEFDKLIVDKDAESSAA
                     QNR"
     gene            1575..>2794
                     /locus_tag="VF870_14880"
     CDS             1575..>2794
                     /locus_tag="VF870_14880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX9253527.1"
                     /translation="MKYENPLDIPLKKAAFTVVDVETTGLSANKNRIIEIALVKIEKL
                     KITDKLHYLINPQTYIPPFITSLTGIENDDVIGAPVFSEIVDEVISFTDNTVLTAHNF
                     PFDSSFLNSEFMLSGREFINEHSCCTLKIARKIYPTLKSKSLTSVAQSLNLKNSNAHR
                     ALGDAEITAKVLLKMIKELQTKDKITTVGELLSYQKGISESPLLNVKKELQEDFRALP
                     HASGIYYFTNKKDEIIYVGKAKSIRERVKTYFSPSAPRKAQKIIKQAARLKHVITNSE
                     LTALLTEAETIKLLEPKHNSQLKKFGNKYFIRITRTHQAPRIELTNHFDFDGNDYFGL
                     FISRRKATEILEFIDKAFAIRECSDKDFKKGKTCFLYDIHRCTGPCVEVELNKENYHD
                     ELEKVYEFLYGKNQ"
BASE COUNT          943 a          483 c          445 g          923 t
ORIGIN      
        1 ccgccaactt ctttgtaata tccttttaaa actttaggaa tatcagtgta ttcagatttt
       61 atattaaaca ttggttttcc atttgtgtag taaagaagtt cctgccgaat tgccatccag
      121 ttatgttgtg taccgtatcc acgctgattt ctcatagtaa taaagctgcg ggaagataat
      181 ccagttgatt tcatcatttc aataaatccg ccaaacggtt caaagtgatt tgactgatct
      241 gccccaagcc aaaaataaaa agatgaattc ttatcagaaa tttccttgtt tagagtaatc
      301 catctcttac accacttgat aaaatcgtca ttttgttttt cagtaaatgc gataaaattg
      361 tatggcggat cctgaacaga aagctttact ttactttttc caaaaatctt ttttacttca
      421 tcacttttgc tgacatccaa acaggcaatt ttgtgtcctg ctttttcatc aatccagatt
      481 gttccctctt taaatctgga ataaggaaga agcagttttc ttagcttttc atccttatct
      541 attctgggca gcggatgatt cagcatcttt atccacaatc agtttatcaa attcagggaa
      601 aaatattctt tctaattcta atgtttttcc tgtttcagca tcaattttaa aaaacattcc
      661 tgttaagtga acgttgtctt ttgcagtttc atacttttgc ggagtcgcaa ataaaaacct
      721 gtttactgcg gcggaagtct tcattccgat taccgaatca taaggtcctg tcattccgac
      781 gtcagtaatg taaccagtgc catttggaaa tattctttca tccgaagttt gaatgtgtgt
      841 atgagttcca accatagcac taactttgcc atctagataa tgacccatgg ccattttctc
      901 tgcagttgct tctgcatgaa aatcaacaaa aacaactttt gtttcctgtt taattcgggt
      961 aagaatccaa tccattgttc tgaatggaca ttcaatagga gtcataaaag tcctgccctg
     1021 taaactaatg actgcaactt ttcctttttt ggtatcagca atgtgataac catttccgtg
     1081 agtacctttt ggatagttaa tcggacgaag tgaacgaggt tctgctttta aataatcctg
     1141 tgattgatgt ttgtcccagg tatgatttcc accggtgatt acatttacgc caagattaaa
     1201 aagttttggt ccttctttat cagtacagcc tttgccatct gaaatatttt caccgttaac
     1261 aacaacaaaa tcagctttat atttttggat caatcccggc agccatgttt gaactatatc
     1321 cataccaggc tgaccaataa tatcaccaac gaaaagtaaa ttaattgaag acatgttttc
     1381 ctaaatcgtt taaagaaata tatctaaaat gagagtaatt gagaaataaa gatcccaaca
     1441 ttgatgcttt aaagtcatcc tgaaattatt tcagaaacta tcaaaagatt caggaagaag
     1501 ttcggaatga gtgtaaatga ataagaccga tcttattact ttgaatgaaa tgattttata
     1561 agttagattt aaatatgaaa tatgagaatc ctttagatat tcctttgaag aaagctgcat
     1621 ttactgttgt ggatgttgaa acaacaggct tatctgcaaa taaaaatcgt attatcgaaa
     1681 ttgctttggt taagattgag aaattaaaga taaccgataa acttcattac ttgataaatc
     1741 cgcagactta tattccaccg ttcattactt cgttaaccgg aattgaaaat gatgatgtaa
     1801 ttggagcgcc agtattttct gaaattgttg atgaagtaat ttcttttact gataatacag
     1861 ttttaactgc acacaacttc ccttttgatt catctttctt gaattcagaa tttatgctaa
     1921 gcggaagaga atttattaat gagcattcct gctgcacttt aaaaatagcc agaaagattt
     1981 acccgacttt aaaaagtaaa tcacttacat cggttgctca atcattaaat ttaaaaaatt
     2041 caaacgcaca tcgcgctctt ggagatgcag aaataactgc gaaagttctt ttaaaaatga
     2101 ttaaagaact tcaaacgaaa gataagataa ccacagtcgg ggaattactt tcatatcaaa
     2161 aaggaatttc tgaatcacct ctattaaatg ttaaaaaaga attgcaggaa gattttagag
     2221 ctttgccgca cgcttcagga atttattatt tcacaaataa aaaagatgaa ataatttatg
     2281 ttggtaaagc aaaatccatt agagaaagag ttaaaactta tttttctccc tcagctccgc
     2341 gtaaagcaca aaagattatc aagcaggcag caagactaaa acatgtaatt acaaacagtg
     2401 aattaacagc tttactaact gaagccgaaa ccataaaact tttagaacca aagcataatt
     2461 ctcagttaaa aaagtttggc aataaatatt ttatcagaat tacacgcact catcaggcac
     2521 caagaattga attaacgaac cattttgatt ttgatggtaa cgattatttt ggattattta
     2581 tttcacgaag aaaagccaca gagattttag aatttattga taaagcgttt gccattagag
     2641 aatgctcaga taaagatttt aaaaaaggta aaacttgttt tctgtatgat attcatcgct
     2701 gcacaggacc ttgcgttgaa gttgaattaa ataaagaaaa ttaccacgat gaattagaaa
     2761 aagtttatga gtttctttat ggtaaaaatc aatt
//
LOCUS       DASWBY010000823         4413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_148292, whole genome shotgun sequence.
ACCESSION   DASWBY010000823 DASWBY010000000
VERSION     DASWBY010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148292"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..714
                     /locus_tag="VF870_14885"
     CDS             301..714
                     /locus_tag="VF870_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253528.1"
                     /translation="MNIPIIDRSNYLKGLFITAKLDKELTDKEKDILKKISDKLGFAS
                     DFFQETIRSLLSNKYIIEEPIIFSDQEIARSFIKDAVKLACVNDLVTEAEISWLKKTA
                     DVNKISIEFVEKEFSSFKETPRSILGSEFALYSII"
     gene            844..1878
                     /gene="tdh"
                     /locus_tag="VF870_14890"
     CDS             844..1878
                     /gene="tdh"
                     /locus_tag="VF870_14890"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX9253529.1"
                     /translation="MKKMKALVKKYAEPGIWMDEVPVPEYGPNDVLIKIHKTAICGTD
                     IHIYNWDEWAQKTIPVPMHVGHEYVGTVEAFGSNVQDVKAGELVSGEGHIVCGTCRNC
                     RAGRGHLCPNTKGVGVNRPGAFAEYLVIPVQNIWHCDPKISTDILSVFDPFGNAVHTA
                     LSFDLLGEDVLITGAGPIGIMAAAVALHAGARNTVITDMNPYRLELARKIGVTRAVNV
                     SKEKLPDVVKELGMKEGFDVGLEMSGSIYAFNDMIDVMFHGGNIALLGILPSNAGVDW
                     TKIIFSGLNIRGIYGRKMFETWYKMQAMVQSGLDINPIITHKFKIDDYLEGFEIMKSG
                     NSGKVILEWN"
     gene            1897..3087
                     /gene="kbl"
                     /locus_tag="VF870_14895"
     CDS             1897..3087
                     /gene="kbl"
                     /locus_tag="VF870_14895"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX9253530.1"
                     /translation="MNTETKQHYTNILNTIENEGLFKKERIITTPQSAHIAVSTGQQV
                     LNMCANNYLGLADNPEIIKAAKDSFDKWGFGMSSVRFICGTQEIHKELEKKISEFLGM
                     EDTILYTSCFDANGGLFETLLGEDEAIVSDELNHASIIDGIRLCKAQRYRYKNCDMND
                     LEEKLKEAKSNNAKNILVATDGVFSMDGFIAPLKNICDIAEKYNAMVMVDDSHAVGFM
                     GKHGKGTHEHNDVMGRIDVITGTLGKALGGASGGYTSARKEIVDLLRQRSRPYLFSNT
                     VAPNIVAASLKVFEMLSATTHLRDKLEENTKYFREKIKAAGFNIKDGVHPIVPIMLGD
                     AVLAQTMAAKMLEKGVYVVGFFFPVVPKGTARIRVQISAAHSKQDLDFAVEKFAEVKK
                     EMNI"
     gene            3156..3641
                     /locus_tag="VF870_14900"
     CDS             3156..3641
                     /locus_tag="VF870_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253531.1"
                     /translation="MKKIMFVFLFVCSISFAQEQKITVGSTELKLGSDLEVVLDLLDK
                     SYYVEEDSKEYNLFSIWDSSKRNFNLGVIKFDKSNKLIFVNKFWGTAVNDSHSQIFEQ
                     IIKLLDTYKAAGEIKVDTKEIFEPNYNAKTLSFIQGNKTIELTLSNSRITLKEILKAS
                     E"
     gene            3689..4120
                     /locus_tag="VF870_14905"
     CDS             3689..4120
                     /locus_tag="VF870_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253532.1"
                     /translation="MKNIALVLVVILTLFACSSKKEYKTPESVIEANAAYMNDEDLEG
                     VMTTVHPKSPSYDGVETMAKQIFERYDLNYKIENIRVLEQNDNEAKVEFTQVTTKIKG
                     PDFKNNKTTGIHTLRKDGDSWKIYSTEMTNVEFLNKDESHN"
     gene            4104..>4413
                     /locus_tag="VF870_14910"
     CDS             4104..>4413
                     /locus_tag="VF870_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX9253533.1"
                     /translation="MNPIIEILKADITKLNVDAIVNTANTLLLGGGGVDGAIHRAAGS
                     KLLEECRKLNGCKTGEAKITNGYNLPAKFVIHTVGPVWNGGNYNEDELLSNYYYNSLK
                     L"
BASE COUNT         1577 a          654 c          842 g         1340 t
ORIGIN      
        1 ttcctgctta attcaatatg ataaacaaag ctcactctat cattttaaag taaaatggtc
       61 caaccctaaa ctaaaatcta ctttttgtgt ttatgtttaa taaataaatc gttagaattt
      121 catttataga tttgagacaa tgtagtcttg aatcgacttc ccatttaaat ggggcaaatc
      181 tttgtatctg gattaatttg tagaatttga tattttagtc tttgttactt tacaggcaca
      241 atagttgaaa ggttagccca tagtttttgt tacaattctt aaattattta ttggatagaa
      301 atgaatattc caatcataga ccgcagcaac tatttaaaag gtttatttat taccgcaaaa
      361 cttgataaag aacttactga caaagaaaaa gacattctca aaaaaatctc agacaaatta
      421 ggtttcgcat cagacttttt tcaggaaaca ataagaagcc tactgtccaa taaatacata
      481 attgaagaac caattatttt ttcagatcag gaaattgcac gttcttttat aaaagatgca
      541 gtaaaacttg cttgtgtaaa tgacctcgta acggaagcag aaattagttg gctaaaaaaa
      601 acagctgatg ttaataaaat cagcattgag tttgtggaga aagaatttag ctcattcaag
      661 gaaactccac gttcaatatt aggaagtgaa tttgcactct attcaattat atagcattaa
      721 atcgaataga aaaatcattt tattttcttt gccctttact ccccttattt ctaattttga
      781 ttagaacatt ttaataaata ttcctaatca aaaaatgtat ttacttttct tgaggatctt
      841 accatgaaaa aaatgaaagc actagtaaag aaatatgctg aacccggaat ctggatggat
      901 gaagttcctg ttcctgaata cggacccaac gatgtactaa ttaaaattca taaaacagca
      961 atttgtggaa cagatatcca catctacaac tgggatgaat gggcacaaaa aactattccg
     1021 gttccaatgc atgttggtca cgaatatgtt ggtactgttg aagctttcgg atcgaatgta
     1081 caagatgtaa aagcaggtga actggttagc ggtgaaggac atattgtttg cggtacatgc
     1141 agaaactgtc gagctggccg tggtcacctt tgtcccaata caaaaggagt cggtgtaaat
     1201 cgtccaggtg cttttgcaga atatttagta attccagttc aaaatatctg gcattgcgat
     1261 cctaaaattt caacagatat tttatctgta tttgatcctt ttggaaacgc cgtacataca
     1321 gctttaagtt ttgatctcct tggtgaagat gttttaataa ctggtgcagg accaatagga
     1381 ataatggctg ctgcagttgc tttacatgcc ggtgcaagaa atacagtaat tactgatatg
     1441 aatccataca gacttgaatt agcaagaaaa attggtgtaa cgcgtgctgt aaatgtttct
     1501 aaagaaaaac ttcctgatgt agtaaaagaa ctcgggatga aagaagggtt tgatgtcgga
     1561 ttagaaatga gcggaagtat ctatgcattc aatgatatga ttgatgtaat gttccatgga
     1621 ggaaacatag cattgcttgg tatccttcct tcaaatgccg gtgttgactg gacaaaaatt
     1681 atttttagcg gattaaacat tcgcggaatc tatggaagaa aaatgtttga aacctggtat
     1741 aagatgcaag caatggtgca gagcgggttg gatataaatc caattataac tcacaaattt
     1801 aaaattgatg actacctgga aggatttgaa ataatgaaat ccggaaattc aggtaaagtt
     1861 attttggaat ggaattaaaa ttttaaagag agaaaaatga atactgaaac caaacaacac
     1921 tacactaata ttctaaatac cattgaaaat gaaggcttat tcaaaaaaga aagaattata
     1981 acaacgcctc aaagcgctca cattgctgtt tcgaccggac agcaagtatt aaatatgtgc
     2041 gcgaataatt atctgggatt agcagataat cctgaaataa ttaaagcagc caaagatagt
     2101 tttgataaat ggggatttgg aatgtcttca gtaagattta tatgcggcac tcaggaaatc
     2161 cacaaagaac tggaaaagaa aatttctgag tttctaggaa tggaagatac aattctttat
     2221 acatcctgct tcgatgcaaa cggcggattg ttcgaaacct tgcttggtga ggatgaagca
     2281 atagtaagtg atgaattaaa ccatgcgagc atcattgatg gaataaggct gtgtaaagct
     2341 cagcgttacc gatacaaaaa ttgtgatatg aatgatcttg aagaaaagct taaagaagca
     2401 aaatcaaata acgcaaagaa tattttagtc gctactgatg gtgtgttttc tatggatggc
     2461 tttattgcac ccttaaaaaa tatttgtgac atagcagaaa agtataatgc aatggtgatg
     2521 gtggatgata gccatgctgt tgggtttatg ggaaaacatg gtaaaggtac tcatgagcac
     2581 aatgatgtga tgggaagaat tgatgttata accggaactc ttggcaaggc acttggtggt
     2641 gcaagcggag gctacacatc agcaagaaag gaaattgtag atttacttcg ccagcgttca
     2701 cgcccatatt tattttctaa tacagttgct ccgaatattg ttgccgcctc actaaaagtt
     2761 tttgaaatgc taagtgcaac aacccactta agagataaac ttgaagaaaa cacaaaatat
     2821 tttagagaaa aaataaaagc tgcaggattc aatattaaag atggagttca cccaatagta
     2881 ccaataatgc ttggtgatgc tgttttggct caaactatgg cagcaaagat gttagagaaa
     2941 ggtgtttatg ttgttggatt cttcttccct gtcgttccca aaggtacagc cagaattaga
     3001 gtacaaattt ctgcagcaca ctcaaaacag gatttggatt ttgcggttga gaaatttgca
     3061 gaagtgaaga aagaaatgaa tatttaattg cattgcattt aatttttcaa tatgtacggt
     3121 acacaaaatt tttataaaat aaattattaa gcgttatgaa aaaaataatg ttcgtttttt
     3181 tatttgtttg ttcaatatct tttgctcagg aacagaaaat taccgtgggt agtactgagc
     3241 taaaattagg atcagattta gaagttgtac ttgatttact tgataagtct tactacgttg
     3301 aggaagattc taaagaatat aatttatttt cgatttggga ttcaagtaag cgaaatttta
     3361 atttaggtgt cattaagttt gataaatcta acaagctgat ttttgtgaat aaattttggg
     3421 gcaccgcagt taatgacagt cacagccaga tttttgaaca aatcattaaa ctgcttgata
     3481 catataaagc tgcgggtgaa ataaaagttg ataccaaaga aatttttgaa cctaattata
     3541 atgcaaaaac attaagtttt atccagggta acaagacaat tgaacttact ctgtcgaaca
     3601 gcagaattac ccttaaagaa attttaaagg cttcagaata gcctatactg ttaattcaaa
     3661 ttatttttaa ttaaacctcc ggaaaaaaat gaaaaatata gctctggtac tcgttgttat
     3721 tttaacatta tttgcttgct cgagtaaaaa ggaatataaa acaccagaat cagttattga
     3781 agcaaatgct gcttatatga atgatgaaga tttagaaggt gtaatgacaa ctgttcatcc
     3841 taaaagccca tcttatgatg gagttgaaac aatggcaaag caaatttttg agagatatga
     3901 tttgaattat aaaattgaaa atatcagagt tcttgaacag aatgataacg aagcaaaagt
     3961 tgaattcaca caggtaacaa ctaaaataaa aggacctgac ttcaagaata ataaaactac
     4021 cggaatacat acacttagaa aagatggtga ctcctggaaa atctattcga ctgaaatgac
     4081 caatgttgaa tttctaaata aagatgaatc ccataattga gattcttaaa gctgatataa
     4141 ccaaacttaa tgtcgatgca attgtaaaca ccgctaatac tttactactt gggggaggcg
     4201 gagtagatgg tgcaattcat cgggccgcag gttccaaact acttgaggag tgcagaaagt
     4261 tgaacggttg taagaccggt gaagcaaaaa tcacaaatgg atataacctg cctgccaaat
     4321 ttgtaattca tactgtgggt cctgtttgga atggtggaaa ttataacgag gatgaattac
     4381 tttccaatta ttattacaac tcattaaagt tag
//
LOCUS       DASWBY010000824         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_261142, whole genome shotgun sequence.
ACCESSION   DASWBY010000824 DASWBY010000000
VERSION     DASWBY010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261142"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VF870_14915"
     CDS             <1..193
                     /locus_tag="VF870_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253534.1"
                     /translation="IDILIPDSVGCEIKSDAALSSRNYEGFIKINNDLYRSENFDKFT
                     KKIYIDIDCGVSSIDVKIY"
     gene            complement(264..617)
                     /locus_tag="VF870_14920"
     CDS             complement(264..617)
                     /locus_tag="VF870_14920"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX9253535.1"
                     /translation="MKKQKTSIRKKEKSKNDFFNKVYRITKKIPYGKVSTYGDIAEAC
                     GIRSSARTVGWALNGCGPDIPAHRVVNRFGALTGKIHFGDPNLMEELLRSEGVEFDKN
                     GCVKLEKHLWKPKKN"
     gene            693..1703
                     /locus_tag="VF870_14925"
     CDS             693..1703
                     /locus_tag="VF870_14925"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX9253536.1"
                     /translation="MASLKRKLLKYKIWFRELFRFCPVSKLKVDRENLFLVCGHRGSP
                     VNEPENTIPSFERALLEGVNSLETDLCVTKDKEVILWHDWNPDELVAIIREQGIEPEV
                     AYKPDFPAPFSGLRNKTSELTLSEIRKNFNYVRKGESISVNAHIPTLREFIDWIKDKN
                     QIKYVFLDIKVPKDEKELVIVILDQLDTLIKEFSLSTKFIIETFYSEVLEIAKKKYPE
                     LNYSLDVEIPPGFIFLPRIHSTVKVAAANKNNVAIIMRPRQITFASWVTFRRVIWYDV
                     RRKKLINKKSDSTKIDFLVGCTINNKKEMECLIKSGISGIQTDYPRRLINVIKEYRLR
                     IK"
BASE COUNT          608 a          247 c          292 g          558 t
ORIGIN      
        1 tattgatatt ctgattcctg attcagttgg atgcgaaata aaatcagatg cagcactatc
       61 cagcagaaat tatgaaggct ttattaagat taataatgat ttatatcgct cagaaaactt
      121 tgataaattt acaaaaaaga tttatatcga tatagattgc ggtgtttcat caattgatgt
      181 taagatatat taaatcttct actacttggg attagtataa ttaaaagtcc ttcccttttt
      241 aaaagggaag gatttagcat gtgttaattt tttttcggtt tccacaaatg cttttccaat
      301 ttcacacaac catttttatc aaattccaca ccttcacttc gtaaaagctc ttccattaaa
      361 tttggatcgc caaaatgaat tttaccggtt aatgctccaa atctattaac aactctgtgt
      421 gcaggaatat ccggaccaca gccatttaat gcccatccaa cagtacgtgc agaagaacgg
      481 atgccgcaag cttctgcaat atcgccataa gtagatactt ttccgtaagg tattttttta
      541 gtaatcctat atactttgtt gaagaaatca tttttagatt tttctttttt tcgtatagat
      601 gttttttgct ttttcattat ataaaaactt taatttttat cttaaacaag attaaaataa
      661 tttgtgatat aaaatcagaa taataaataa ttatggcaag tttaaaacga aaattactca
      721 agtataagat ctggtttaga gaacttttta gattttgccc ggtcagtaaa ttaaaagttg
      781 atagagaaaa tctgttttta gtttgtggac atcgcggctc acctgtcaat gaacctgaaa
      841 acacaattcc gtcttttgag agagcattgc tcgaaggagt aaattcactt gagactgatc
      901 tttgtgtcac aaaagataag gaagtaatac tctggcacga ttggaatcct gatgagttgg
      961 tcgcaattat acgcgagcaa ggaattgaac cagaggttgc ttataagccc gacttccctg
     1021 ctccatttag cgggttaaga aataagacaa gcgaattaac attatccgaa ataagaaaaa
     1081 attttaatta tgtgagaaaa ggtgaatcaa tttcggttaa tgctcacatt ccaaccttaa
     1141 gagaatttat agattggata aaagataaaa atcagatcaa gtatgttttt ttggatatca
     1201 aagttccaaa agatgagaag gaattggtta ttgtgatact tgatcaactt gatactttga
     1261 taaaagaatt tagtctatct acaaaattta taattgaaac attttactcc gaggtattgg
     1321 aaattgccaa gaaaaaatat ccggagctta attatagttt agatgttgag attcctccag
     1381 gatttatatt tttacccaga attcacagta cagtaaaagt ggcagctgca aataaaaata
     1441 atgttgcaat aattatgcgg cccagacaga ttacatttgc cagctgggta acttttagaa
     1501 gagtgatatg gtatgatgta agaagaaaga aattaataaa caaaaaatct gattcaacta
     1561 aaatagactt tctggttggc tgcaccatta acaataaaaa agaaatggaa tgtttgataa
     1621 agagcggtat aagcggaata caaactgatt atccaagaag acttattaat gttattaaag
     1681 aatataggtt aaggataaag tagtt
//
LOCUS       DASWBY010000825         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_185758, whole genome shotgun sequence.
ACCESSION   DASWBY010000825 DASWBY010000000
VERSION     DASWBY010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185758"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VF870_14930"
     CDS             complement(<1..462)
                     /locus_tag="VF870_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX9253537.1"
                     /translation="MNTKYEAVIGLEVHTQLLTDSKIFCGCSTKFGNDPNSNTCPVCL
                     GHPGVLPVLNKKVVEYTALMGLATNCRINEHSVFARKNYFYPDLPKGYQISQYEEPIC
                     ETGFIEITAKTGSTKKIGITRIHMEEDAGKSIHDHSSATLVDVNRCGVPLME"
     gene            626..2131
                     /locus_tag="VF870_14935"
     CDS             626..2131
                     /locus_tag="VF870_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX9253538.1"
                     /translation="MQRIQKLGFIVGLISFLIVLIFFNPDPQNPNVGKVAAVAVLMAV
                     WWITEALPMAATSLVPLVFFPLLGVLSGSEISGSYINSVRFLFIGGFMLAIAMENWGL
                     HKRIALKIISIFGGTANSILFGFMFSATFLSMWISNTATAVMMLPIALAVITKIENEF
                     GKDETKNFSKSVLLGIAYSCSIGGIATLIGTPPNLALVRIHKIAFPNAPQISFGDWML
                     LAFPITVILLILTAILLSKIIFRSKKSIKISKDFIANEYNSLGKFSFPEKAIAVIFSI
                     TSLLWIFRTDLNLGFIKITGWSSVFSTPEFIDDGTIAITMAFLLFLIPSKDEKQKTIL
                     GSNVFEQIPWGIILLFGGGFALATAFSSSGLSQFIGNNLRGLSHVPTFILVLIICFII
                     NFLTELTSNTATTQMILPILASVSVAIGINPLLLMIAATLSASMAFMMPVGTPPNTIV
                     FASKRLRISDMAKTGFVLNIISVILISILVYFIGSIIFDLNTFPDWAKISK"
     gene            2283..3380
                     /locus_tag="VF870_14940"
     CDS             2283..3380
                     /locus_tag="VF870_14940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253539.1"
                     /translation="MKLKIVGILLALICQSILAQTASAYFPSQAGYKWSYESIPLDSL
                     NNPVNSQKFFSIDSFAVVTPFNGKNSNVVLSKTGPEITINFQPFLDTSYYSFESSNGF
                     EYFDPKSVYDLLGNVDTTLGINFINFFNSLEGWYPYYRFASANNVEYQVFKKDTAVTV
                     NSVAATIRFELIGKKLADETINTAIGNFLCKKFVMEVRISYLLLNIFPVKLFGVEDSL
                     WFAPDNWKVRSFIPATNVDLSILGIPAFYIPGLETNIITPITNLGSEINAINNFELYQ
                     NYPNPFNPSTIIQYALGSREFVQLKVFDVLGNELATLVDEEKPAGNYEVEFTVSKFAD
                     GRSRPAIASGVYYYQLKVGNLTQSKKMILIK"
     gene            3445..>3763
                     /locus_tag="VF870_14945"
     CDS             3445..>3763
                     /locus_tag="VF870_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253540.1"
                     /translation="MFKYDLISKLFSTEAQTIEVPTRDEIEEQYKWNLKDIYSSDAEW
                     EKVFKFVEQSIIGYAQYEGRITDSAENLLACLKFDEEIGIKLERLYLYSMLAKDSNMR
                     DPKY"
BASE COUNT         1205 a          596 c          639 g         1323 t
ORIGIN      
        1 ttccattaac ggaactccgc atctgttcac atcaacaagc gtggcactgc tgtgatcgtg
       61 aatagattta ccggcatctt cttccatatg gattctcgta atcccgattt ttttagtcga
      121 accagtttta gcggtgattt ctataaaacc ggtttcacaa atcggttctt catactgaga
      181 gatttgatat ccttttggaa gatcgggata gaaataattt tttcttgcaa atacggaatg
      241 ctcgtttatc ctgcaattag tagccaatcc cattagagca gtgtactcga caaccttttt
      301 gttaagcaca ggcaatactc cgggatgacc taagcacact ggacaagtat tgctgttggg
      361 atcattacca aacttagttg aacatccgca aaaaattttt gagtctgtta aaagctgggt
      421 gtgaacttct aaacctatta ccgcttcgta ttttgtattc ataaaatgtg attcttttcg
      481 caactaaata tatggatgat aatctttata tagaaagatg aaaataaatc tattatgata
      541 gctggattaa atgttagacc tgcggtttta tattacttta gttataagta tttttttaca
      601 gcttttaata aattgatatg attttatgca gagaatacaa aaattaggat ttattgttgg
      661 gttaatatcc tttctgatag tcttgatttt ttttaatcct gatcctcaaa acccgaatgt
      721 aggtaaagtt gccgcagttg ctgttctaat ggcagtctgg tggattacag aggccctgcc
      781 aatggctgca acttcattag tacccttagt cttcttcccg ctgctgggag ttttatctgg
      841 ttcagaaatt tccggttcat acataaactc cgtaagattt ctgttcattg gcgggtttat
      901 gttagcaatt gcaatggaaa attggggatt gcataagcga attgccttaa aaataatttc
      961 aattttcggc ggcacggcca attctatttt atttgggttt atgttctcag ctacttttct
     1021 ttctatgtgg atttcaaaca cagcaactgc tgttatgatg cttcctattg cacttgctgt
     1081 aatcacaaaa attgagaatg aatttggaaa agatgaaaca aaaaattttt caaaatcagt
     1141 attgcttggc attgcttatt catgttcaat cggtggaatt gcaacattaa ttggaacccc
     1201 tccgaattta gctttggtgc gaattcacaa aatagctttc ccaaatgcac ctcaaatatc
     1261 tttcggtgat tggatgttat tggcgtttcc aattacagta attttattaa ttcttactgc
     1321 aatactttta tctaaaataa ttttcagatc aaaaaagagc atcaagatca gtaaagattt
     1381 tattgcaaac gagtataatt cactcggcaa gttttctttt cctgaaaaag cgatcgcagt
     1441 tatttttagt ataacttctt tactgtggat ttttagaact gatttaaatc ttggctttat
     1501 aaaaataaca ggctggtcat ctgttttttc aactccggaa tttattgatg acggaactat
     1561 tgcaatcact atggcttttt tactttttct gattccatca aaagatgaaa aacaaaaaac
     1621 tattctggga tcaaatgtat ttgagcagat cccctggggt ataattcttt tattcggtgg
     1681 tggatttgca cttgcaactg catttagttc aagtggatta tcacagttta taggaaataa
     1741 tttgcgagga ttgtctcacg tcccaacatt tatattggta ttaattattt gtttcataat
     1801 taattttcta accgagttaa catcaaatac agcaacaact caaatgatac ttccaatact
     1861 tgcatccgtt tcagttgcaa tcggaataaa tccattatta ttgatgattg cagcaactct
     1921 gtctgcctca atggctttta tgatgccggt tggaacgcct ccaaacacaa tagtttttgc
     1981 aagcaagcga ctgagaatat ctgatatggc aaaaacagga tttgttctta atatcattag
     2041 tgtaatttta atatcaattc tagtttactt tatcggttca atcatttttg atttgaatac
     2101 ttttcctgat tgggccaaaa tttctaaata gttcaaaacc ataaagtttt gttaaaatca
     2161 ataaaaaaca tattaatttt atagctcttg ttgatggggg caaaatttat tgatagtcaa
     2221 atactcgtaa taaaaaatta gttttcttat attactggtt gattaaaaaa cacttggata
     2281 aaatgaaatt aaaaattgtt ggaatattat tagcgttgat atgtcagtcg attttagcac
     2341 aaacagcttc agcatatttt ccttcacaag ccggctataa atggagttac gaatctattc
     2401 cacttgattc actaaataat ccagtgaact cgcaaaagtt tttttcaatt gattcgtttg
     2461 ctgttgtaac acctttcaat ggaaaaaatt caaatgttgt tttaagcaaa accggacctg
     2521 aaatcacaat caattttcaa ccttttcttg acacatctta ctacagtttt gaatcatcaa
     2581 atggatttga atattttgat cctaaaagtg tttatgattt attaggtaat gtggatacga
     2641 cacttggaat taattttata aactttttca attcactgga gggctggtat ccttattatc
     2701 gattcgcatc agcgaataat gttgaatatc aggtatttaa aaaagatact gctgtaacag
     2761 taaattctgt tgcggcaact attagatttg aattaatagg gaaaaagctg gctgatgaaa
     2821 caattaatac agcaataggt aattttctct gcaaaaaatt tgtaatggaa gtaagaataa
     2881 gttatttatt gttgaacatt tttccggtta aacttttcgg tgttgaagat tctctttggt
     2941 ttgcacctga taattggaaa gttagaagtt ttattccggc taccaatgtt gatttatcaa
     3001 ttttaggtat tccagctttt tatattccag gcttagaaac aaacatcata actcctatca
     3061 caaatttggg atctgaaatt aatgcgataa ataattttga actctatcaa aattatccaa
     3121 atccatttaa tccttctacc attattcaat atgctttagg aagcagggaa tttgtacaat
     3181 taaaagtttt tgatgtatta gggaatgagt tagcaacact tgttgatgaa gaaaaaccgg
     3241 ctggaaatta tgaagttgaa tttactgttt caaaatttgc cgatggcaga tcccgtcctg
     3301 caattgcaag tggtgtttat tactatcaac taaaagttgg taatctaacc caatcaaaaa
     3361 aaatgattct aatcaaatga ttattttatc ttttatgcaa ttaaaaaata taattaactt
     3421 ttattccact atctggggat acagatgttc aagtatgatc taatatcaaa attatttagc
     3481 acggaagctc aaacaatcga agttcccacc cgggatgaaa ttgaagaaca gtataaatgg
     3541 aatctgaaag atatttatag ttcggatgcg gaatgggaaa aggtatttaa atttgttgag
     3601 caaagtatta ttggatatgc acaatatgaa ggtaggataa ccgattcagc agaaaatctt
     3661 ttggcttgtc ttaaatttga tgaagagata ggaatcaaat tagaaagatt atatctctat
     3721 tctatgcttg ccaaagacag caatatgaga gacccaaaat atc
//
LOCUS       DASWBY010000826         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_749145, whole genome shotgun sequence.
ACCESSION   DASWBY010000826 DASWBY010000000
VERSION     DASWBY010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749145"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VF870_14950"
     CDS             <1..859
                     /locus_tag="VF870_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9253541.1"
                     /translation="IDYQLIVDDSTITDPRNPIVTPSGFGPHSQCAMPLFGIKSVREH
                     RNNIQHGTIDTLWFKSRQTSIMPRMLKIYKPANYDSLFSLPTLYVNDGFKAIEYNSYI
                     NILDNLIADKKIKHILVIFIDFIEGDQNLFLNKTDKYFKSVCDELVPLIDANYKTSQL
                     AKDRVLAGISAGGHISLLTSLKRSDIFLNAAGQSSTITEELFETVKNISKTNPNKKEL
                     KLYFDVGRYDLLNGAINNYSFLYANQLLDKEMKKAGINHTFKIVNDGHQWANWRESVD
                     EILIYFFNK"
     gene            900..2267
                     /locus_tag="VF870_14955"
     CDS             900..2267
                     /locus_tag="VF870_14955"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9253542.1"
                     /translation="MSAKLILLITTLTAFMTAFMGSSINIALPIIGIEFNSNALMLSW
                     LSTSYLVTTAALLLPVGRLTDIFGRTKFFKWGIVLFTMGSILSGAAPNTITLLLFRIL
                     QGVGSTFIFSTSTALLVSVFPQSERGKVLGINTAAVYIGLSSGPFGGGIITYNLTWRG
                     IFFINAFIGIALIILAWIYLKHEWQEIQIHKYDYRGAAIYIFSMLLLMLAVSLFPKPV
                     GFILLGISLISLITFYYSEKTTNDPVLNTELFSSNRTFSLSNLAALINYSATFAISFL
                     MSFYLQYVRNLTPQDAGIILITQPVLQALFSPIAGKLSDKTEPRFVASAGMFLLTIGL
                     LFFCFLNTETSYILIVSNLAMMGFGFALFSSPNVNAIMSSVEKKHYGVASSILASMRM
                     IGQMLSMGIVIIIFNIFIGPEKISSVNQKNFLISSNTAFILFSVLCFVGIFASLSRGK
                     IHSNK"
     gene            2336..3331
                     /locus_tag="VF870_14960"
     CDS             2336..3331
                     /locus_tag="VF870_14960"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX9253543.1"
                     /translation="MNNLNAMIYAASGALEFIFNPTSEDKILILTDTYSALIANAFKH
                     ASLKIGCGVESYEINNHDRPLKEIPKELEKMLAGKTIVLNIIKAYSEEIAFRIKWLFK
                     VEENKLIKMGHMPGITEEMMLRSVNVDFVTMKTTADKLISELKAATHLHITTEEGTDI
                     LLGVKGRMFSGDIGVSGGEMCNLPCGEIYCAPIETEADGVVVFNASIGDIGMFEHPLK
                     VFVSKGRIIKFESDNEDLVKRITELTSVDDDAWVIGELGIGVNPGAKITGNMLEDEKA
                     ICTAHIAFGNNADFPGGGKNNSKIHRDYLFYRPNIKAIFPDSTEKHIMSNGEVVL"
     gene            complement(3346..>4642)
                     /locus_tag="VF870_14965"
     CDS             complement(3346..>4642)
                     /locus_tag="VF870_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253544.1"
                     /translation="IENLFLPLDMNKSSFRGKEKYKDDIYKGYVYLNGKQNEFPFDFI
                     NDYPAGQMLTTINEFSNFMIMQLNGGMFEGRKIIDSLFIDEMQSVQFTHHPKLHSAVG
                     YGFFIDEVNGTRLLSHNGGYPGILTRMLIFPELKLAMFIAMNGYNSNFNAIITDAVMN
                     KFFPYQAPESKTKYPLTNLPEYDKNVDKFIGNYRFTRYSRNEIAKIGLLMGMIGAELP
                     IWKNDDGMLMMYDLNNKARRLIQVEPLLFQSIDDDYYIKFREDENGNITHLFTDGITS
                     LEKTPLFYTINFQRIVFAVVTIVFVLISLGGLVRSVIKKIKKSGNKKSNMFKLAEKIS
                     NTYLIYLLLFGLAMNVFLNPLEQMVGFPYGMPLYFYLFQLMPFVFIALTVLFVVKFFS
                     EIRNTPKLKFSIVFNGLFMIVCFTTIWFMNTWNLIGFKF"
BASE COUNT         1562 a          769 c          778 g         1533 t
ORIGIN      
        1 aattgattac caattaattg ttgatgacag cacaattacg gatccacgta acccaattgt
       61 aactccaagc ggatttggtc ctcactccca atgtgcaatg cctttgtttg gaattaaatc
      121 tgtaagggaa cataggaaca acattcaaca cggaacgatt gatactcttt ggtttaaaag
      181 ccgtcagaca tcaataatgc cgagaatgtt aaaaatttat aaaccggcta attatgattc
      241 tcttttttct cttcctacgc tttacgttaa tgatggattc aaggcaatag aatacaactc
      301 ttatataaat attcttgata atcttatagc agataaaaaa attaaacaca tacttgtcat
      361 ctttatagat tttattgaag gtgatcagaa tttatttctt aataaaacgg ataaatactt
      421 taaatcagtc tgcgatgagc ttgtcccatt aattgatgca aactataaaa cctcacaact
      481 tgcaaaagac agagtgcttg ccggaatctc agcaggcgga cacatttcgc ttctaacatc
      541 gcttaaacgt tctgatatat ttttaaatgc cgccggtcag tcttctacaa taacagaaga
      601 gctatttgaa acagtaaaaa atatttccaa aaccaatcca aacaaaaaag aacttaaatt
      661 gtattttgat gttggtagat atgatctttt gaatggggcg attaataatt attcgttttt
      721 atatgcaaac cagttattag ataaagaaat gaaaaaagca ggaattaatc atacatttaa
      781 aatcgttaac gatgggcacc agtgggcaaa ttggagagag agtgtggatg aaatattgat
      841 ttattttttt aataagtaaa ttaatttcca aaacagatat gaaatcagcc ccctcgcaga
      901 tgtcagcaaa gttaattctg cttattacaa cacttaccgc gtttatgact gcctttatgg
      961 gttcatcgat taatattgca ttaccaataa taggaataga gtttaattca aatgctttaa
     1021 tgttaagctg gctttccact tcttatcttg ttacaaccgc agcactgctc ttaccggtag
     1081 gaagattaac cgatattttt ggtaggacta aattttttaa atggggtata gttttattca
     1141 caatgggatc tattttgagc ggtgctgccc caaatactat aacactttta ttgtttagaa
     1201 ttttacaagg ggtgggttca actttcattt tcagcacctc aaccgcttta ctggttagtg
     1261 tatttccaca atccgaaaga ggcaaggtct taggtataaa tactgctgct gtttatatcg
     1321 gactttcatc agggccattt gggggcggaa taattactta taatttaacg tggcgcggga
     1381 tctttttcat caatgctttt attggaatag ctctgataat tttggcatgg atctatctta
     1441 aacacgaatg gcaagaaata cagatccaca aatatgatta cagaggtgct gcaatttata
     1501 ttttttcaat gctgctttta atgttagctg tttcattatt cccaaaacca gtaggcttta
     1561 ttttgcttgg tatatcgtta ataagcttaa taacatttta ttatagtgaa aaaacaacaa
     1621 atgatcccgt tttaaacact gaactattca gttcaaacag aaccttttct ctctcaaatc
     1681 tagcagcgct tataaactat agcgcaactt ttgcaataag ttttttaatg agcttttatt
     1741 tgcagtacgt caggaattta acaccacaag acgcagggat aattttaatc acacaacctg
     1801 tgttgcaagc attattttca cctattgccg gtaaattatc tgataaaact gaaccaagat
     1861 ttgttgcctc ggcgggaatg tttttgctta caataggatt attatttttc tgctttttga
     1921 acactgaaac aagttatatt ttaattgtct ccaatcttgc aatgatgggg tttgggtttg
     1981 cattattctc atccccaaat gtaaacgcta taatgagctc cgttgagaaa aaacattacg
     2041 gagttgcttc atcaatactt gcttcaatga gaatgattgg gcagatgctt tctatgggaa
     2101 ttgtaataat tatttttaac atttttattg ggcccgaaaa aataagttca gtaaaccaaa
     2161 agaatttttt aattagttct aacacagcat ttattttatt cagtgtttta tgttttgtcg
     2221 gaatattcgc ttcgctctca agaggaaaaa ttcacagcaa caaataagtt gttctaaata
     2281 aaagagatat attttatttt tgtgtatgct cttcaaacat tataagagga ctcatatgaa
     2341 taatcttaat gcaatgattt atgccgcatc tggagcttta gaatttattt ttaatccaac
     2401 ctcagaagat aaaattctta tcctcactga tacctattcg gcattaattg caaatgcatt
     2461 taaacatgct tcgcttaaaa ttggttgtgg tgttgaatca tatgagatta ataaccatga
     2521 taggccatta aaagaaattc cgaaagaact ggaaaaaatg ctagcaggaa aaactatagt
     2581 acttaacata ataaaagcat actccgaaga aatcgctttt agaattaagt ggctttttaa
     2641 agttgaagaa aataaactta taaagatggg acatatgccc gggataacag aggaaatgat
     2701 gcttcgctct gttaacgttg atttcgttac aatgaaaaca acagcagaca aactaattag
     2761 tgaactgaaa gctgcaacac atttacacat aacaacagaa gaaggcactg acattctgct
     2821 tggtgtgaag gggagaatgt tttcgggtga tattggagtg tcgggaggag aaatgtgtaa
     2881 tcttccttgt ggggaaattt actgtgcccc cattgaaaca gaagcggatg gtgttgttgt
     2941 ttttaatgcg agcatcggag atattggtat gtttgagcat ccattaaaag tttttgttag
     3001 taaaggtaga atcataaagt ttgaaagtga taatgaagat cttgttaaaa gaattactga
     3061 attaacttct gttgatgatg atgcctgggt aattggtgag cttggtattg gtgttaatcc
     3121 tggcgcaaaa attaccggca acatgcttga agatgaaaaa gcaatctgca cggcgcatat
     3181 agcatttggt aataatgctg atttccccgg tggcggaaaa aacaattcaa aaatacatag
     3241 agattattta ttctatcgtc caaacattaa agccatattt cccgatagta cagaaaaaca
     3301 cattatgtct aatggcgagg tagtcctata agtaatgaca taaatctaaa acttaaaccc
     3361 tatcagattc cacgtattca taaaccaaat agttgtaaag catacaatca taaacagtcc
     3421 attaaaaaca attgaaaatt ttaatttggg agtatttctt atttcgctga aaaatttaac
     3481 cacaaacaaa actgttagtg caataaatac aaaaggcatt aactgaaaca aataaaaata
     3541 caatggcatt ccgtaaggaa agcctaccat ctgttcaagc ggatttagaa atacattcat
     3601 agccaggccg aaaagcaaaa gatatatgag ataagtgttt gaaatctttt ctgccagttt
     3661 aaacatattg ctttttttat ttcccgattt ttttattttt ttaatgacac ttctaaccaa
     3721 tccgcccaac gatattaaaa caaacacgat tgtaacaact gcaaatacta ttctttgaaa
     3781 attaattgta taaaaaagag gagtcttttc aagagatgtt attccatctg taaacaaatg
     3841 agttatattt ccattctcat cttctctgaa tttaatgtag taatcatcat caattgattg
     3901 gaaaagaaga ggttcaactt gaattagtct tcttgcctta ttgttaagat catacatcat
     3961 cagcatacca tcatcatttt tccagattgg aagctctgct cctatcattc ccattaacag
     4021 accaatcttt gcaatttcat ttcttgaata acgtgtaaac ctgtaattcc cgataaactt
     4081 atctacattt ttgtcatatt ccggaagatt tgttaaagga tatttggttt ttgattccgg
     4141 agcctggtag ggaaaaaatt tattcattac ggcatcagtt ataattgcat taaagttaga
     4201 attgtagcca ttcatagcaa tgaacattgc aagtttcaat tccggaaaga ttaacattct
     4261 tgttaaaata ccgggatatc cgccattgtg tgataaaagt cttgttccat taacttcatc
     4321 gataaaaaat ccataaccca ctgccgaatg aagtttggga tgatgagtaa actgcactga
     4381 ctgcatttca tctataaaca aactatcaat aatttttctt ccttcaaaca ttccgccatt
     4441 caattgcatt atcataaagt tggagaactc attaattgtg gtgagcattt gtccagcagg
     4501 atagtcattt ataaaatcga acggaaattc attctgcttt ccattcagat aaacatatcc
     4561 tttataaata tcatctttat atttttcttt tcctctgaaa gaacttttat tcatatcaag
     4621 aggaagaaag agattttcaa tt
//
LOCUS       DASWBY010000827         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_148466, whole genome shotgun sequence.
ACCESSION   DASWBY010000827 DASWBY010000000
VERSION     DASWBY010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148466"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..2017
                     /locus_tag="VF870_14970"
     CDS             32..2017
                     /locus_tag="VF870_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX9253545.1"
                     /translation="MSEHQGGQIKGLPENAYTELKPGEEYKPLMPADSNPQEITVYSV
                     TMGLLMAVLFSAAAAYLGLKIGQVFEAAIPIAIIAVGVSTMLKIKGSLGQNVIIQSIG
                     QNSGLIVAGAIFTIPALYILNLQAEFYQIFLASAFGGVLGMLFLIPFRKYFVAEMHGK
                     FPFPEATATTEILVAGEKGGKQALVLVVSGLIGGFYDFIIATFGWWSETFTTRVVGFG
                     EMLADKFKLVFRLNVGAAVMGLGYIVGLKYAAIIAAGSFVSWYLLVPVISYIGAGLTE
                     PFGTGATKIIAAMSAEEIFRQYVRHVGIGGIAMAGIIGIIRSSKIIRDAVTLAFKEIF
                     EKKTESNTAKRTQLDISMKIIVGGLIITSILLFIFFQMGVVHNLGWAFAGLLIVLIIS
                     FLFTTVAANAIAIVGTNPVSGMTLMTLILSSIVLTSVGLKGESGMVAALIIGGVVCTA
                     LSMAGGFITDLKIGYWLGTTPRKQEQWKFLGVLVSALTVGFVIMILNETYGFVGEGSL
                     VAPQANAMAAVIQPLMDQQPAPWVLYIVGAILALVLTMIKVPALAFALGMYIPLELNT
                     PLLVGGLIAHYVSTRSKDEKVNNARRERGTLIASGFIAGGALMGVISAILRWQGFNWV
                     NPEWAESHSAEILGLVMFALISLYMIWDSLRGKPEAE"
     gene            complement(2099..>3718)
                     /locus_tag="VF870_14975"
     CDS             complement(2099..>3718)
                     /locus_tag="VF870_14975"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9253546.1"
                     /translation="HILMLRSGMVRMLSAGIYSFLPLGYKVMKKVSEIIREEMDAIGG
                     QEFHLPALNPTEIWEETNRVEAFGDTMFHLKNREYVLAPTHEEIMTFHARNVVKSYKD
                     MPQIWYQIQTKFRNEPRPRSGVIRGRQFLMKDAYSFDTSWEGLDKSYELHDKAYRKIF
                     DRCGLKYFVVGASSGAMGGTGSEEFMVRSAAGEDTVAYCESCGYAANVEVAVSKVDAV
                     ERENESKAVYEISTPNVHSIDELCEFLKIDETKCAKSRIYIHNEKPVLILMLGNDEVN
                     ETKLEKVLGGVVRPAHPEELKDISGADAGSIGPVGFNGRIIADNRLKDANNMFSGANK
                     NDYHIGGIDFKRDVPTIEYDDFRIVKSGEGCLRCEKHLQVFPAIELGHIFKLGTKYSD
                     SMGALFTDEKGEEHPIIMGSYGIGVERVMACYIEQNHDANGIIWDKAIAPFQVHLLGL
                     NLKKQEIVDACERIYNDLIKQGIEVLFDDRDVTAGFKFNDADLLGMPIQVVIGEKKLK
                     ENKCEVKVRNSGERFDVELSELINKIKELLK"
BASE COUNT         1070 a          722 c          691 g         1235 t
ORIGIN      
        1 ttaactttaa attctaataa aggaacgtaa tatgagcgaa catcaaggcg gacaaatcaa
       61 aggacttccc gagaatgctt atactgaact taagcccggt gaagaataca aaccactgat
      121 gcctgcagat tcaaatcctc aggaaatcac tgtttactct gtaacaatgg gtttgctgat
      181 ggcagttttg ttttctgctg cggcagctta tctcggttta aaaatcgggc aggtatttga
      241 agctgctatt ccgattgcaa ttattgcagt tggtgtatca actatgctta agataaaagg
      301 atcactcggc cagaacgtga ttattcagtc aatcgggcaa aactccggat tgatagttgc
      361 cggtgctatc ttcacaattc ctgctttgta cattttaaat cttcaggcgg aattttacca
      421 gatattttta gcttctgctt ttggtggagt gttaggaatg ctttttctga ttccttttag
      481 aaaatatttt gttgcagaaa tgcatggcaa gtttcctttt cctgaagcca cagctacaac
      541 agagatatta gttgccggcg aaaaaggcgg caagcaagct ttggtgcttg ttgtaagcgg
      601 attgatagga ggattttatg attttataat cgcaactttt ggttggtgga gcgaaacttt
      661 taccactcgt gttgttgggt tcggtgagat gcttgctgat aaattcaaac ttgtattcag
      721 gttaaatgtc ggcgctgctg taatgggatt aggctatata gtcggattaa aatatgcagc
      781 tataattgca gcaggatcat ttgtttcttg gtaccttttg gtgcctgtaa tttcttatat
      841 aggtgcagga ttaactgaac catttggaac tggtgcaact aaaattattg ctgcaatgag
      901 tgccgaagaa attttcagac aatatgttcg ccacgtggga attggcggaa ttgcaatggc
      961 aggtattatt ggaattatcc gatcatcaaa aattattcgt gatgccgtta cgcttgcctt
     1021 taaggaaatt tttgaaaaga aaactgaaag caacactgct aaaagaactc aattagatat
     1081 ctcgatgaaa attattgttg gcggattaat tatcacttca attttacttt tcattttctt
     1141 ccagatgggt gtggttcaca atctcggttg ggcttttgct ggattattaa ttgtgttaat
     1201 catttcattt ctgtttacaa cagttgctgc aaacgcaata gcgattgttg gaacaaatcc
     1261 tgtaagtgga atgacattga tgacattaat tctttcatca atcgttctta cttctgttgg
     1321 attgaagggt gagtccggaa tggttgccgc attaattata ggcggagttg tttgtacagc
     1381 actttcaatg gcaggtggat ttatcacaga tcttaaaatt ggttactggc tcggtaccac
     1441 cccaagaaag caagagcagt ggaaatttct cggtgttctt gtttcagctt taacggttgg
     1501 ttttgttatt atgatattaa atgaaaccta cggatttgtt ggtgaaggtt cactggttgc
     1561 tcctcaggca aatgcaatgg cagcagttat tcagccgcta atggaccagc agcccgcgcc
     1621 gtgggtgctt tacatagttg gagcaatttt agctcttgtt ttaacaatga ttaaagttcc
     1681 agccctggca tttgctctcg gaatgtatat ccctcttgaa ttaaacactc cacttctagt
     1741 tggcggattg attgctcatt atgtatctac acgcagtaaa gatgaaaaag ttaacaatgc
     1801 aagaagagaa cgcggaacat taatagcttc cggatttatt gcaggcggcg cgttaatggg
     1861 tgttataagt gcaatcttaa gatggcaggg attcaattgg gttaatcctg aatgggcaga
     1921 atctcactca gcagaaatac ttggattagt aatgtttgca cttatttccc tttacatgat
     1981 ttgggattcg ttgagaggaa aaccagaagc agaatagaac tagaagtttc gaaattgaca
     2041 aataaaaatg ccatatcaaa tttgatatgg cattttttaa tattaaatat ttctaattct
     2101 acttcagcaa ctccttaatc ttgtttatca attcgcttaa ctcgacatca aatcgttcac
     2161 ctgaatttct aactttaact tcacacttgt tttcttttag tttcttctcg cctataacaa
     2221 cctgtattgg cataccgagt aaatctgcat cattgaattt aaatccagcc gtaacatcac
     2281 gatcatcgaa taatacttct attccttgtt tgattaaatc attataaatt ctctcacaag
     2341 catctacaat ttcctgcttc tttaaattca gaccaagtaa gtgaacttga aatggagcaa
     2401 tagctttatc ccatataatt ccgtttgcat cgtgattctg ttcaatataa caagccatta
     2461 cacgctcaac accaataccg taacttccca taattattgg atgttcttca cctttttcat
     2521 cagtaaacaa agcgcccata gaatcggaat attttgtacc gagtttaaag atgtgcccaa
     2581 gttctattgc tggaaaaacc tgaagatgtt tttcacatcg caggcagcct tcacccgatt
     2641 ttactattct gaaatcatca tactcaattg ttggtacgtc gcgtttaaaa tcaattccgc
     2701 caatgtgata atcattttta tttgcaccgc taaacatatt gttagcatct ttcaaacgat
     2761 tatcagctat aattcttccg ttaaatccaa caggaccaat agatccggca tctgcaccgg
     2821 aaatatcttt tagttcttcc ggatgagcag gacgaacaac accgcctaaa actttttcta
     2881 actttgtttc attaacttca tcattgccaa gcatcaaaat taaaactggt ttttcattat
     2941 ggatataaat tctagatttt gcacatttcg tctcatcaat cttcaaaaac tcacaaagct
     3001 catcaataga atgaacattt ggtgttgaga tttcataaac agctttactt tcgttttctc
     3061 tttcaactgc atcgactttt gagactgcta cttcaacgtt tgcagcataa ccgcaagact
     3121 cacaataagc aactgtatct tcaccggcag cagacctaac cataaattct tcagagccgg
     3181 ttccgcccat tgcgccgctt gacgcaccaa caacaaaata ttttaatccg cagcgatcaa
     3241 atatttttct atatgcttta tcgtgcagct cataagattt atcaagacct tcccagcttg
     3301 tatcaaagct gtatgcatct ttcattaaaa attgtctgcc gcgaattact ccgcttctcg
     3361 gccttggttc atttctaaat ttagtttgta tctgatacca gatttgcggc atatctttat
     3421 atgattttac aacattgcgt gcgtggaatg tcattatctc ttcgtgcgtt ggagctaaaa
     3481 cgtattcgcg attttttaaa tgaaacattg tatcaccaaa agcttcaaca cgatttgttt
     3541 cttcccatat ttctgttgga ttaagcgccg gtagatgaaa ttcctgtccg cctattgcat
     3601 ccatttcttc gcggataatt tcacttactt ttttcataac tttatatcca agaggaagaa
     3661 atgagtaaat tcccgcagat aacattctaa ccattccaga acgaagcatt aaaatatg
//
LOCUS       DASWBY010000828         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_522911, whole genome shotgun sequence.
ACCESSION   DASWBY010000828 DASWBY010000000
VERSION     DASWBY010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522911"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VF870_14980"
     CDS             complement(<1..642)
                     /locus_tag="VF870_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9253547.1"
                     /translation="MKDIILKVENISYSVDKKTGLSGSSNSYSQSQTVEILKNISFEI
                     ERGKVLGISGQSGSGKSTLAKILAGIFPPTSGIIQNNFKRDWSKSLQKPVQILFQNDG
                     ELINPFRKVKDVLNEAFELKSKQNKVYTSEIDEIFDRFNLNTQLKESKGSQLSGGEKQ
                     RIALARIVIVGPEILVLDEPFSSQDVEAQLGILNLIKKLKEEFNLTIICISHDL"
     gene            complement(639..1388)
                     /locus_tag="VF870_14985"
     CDS             complement(639..1388)
                     /locus_tag="VF870_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9253548.1"
                     /translation="MLKVDLRKIILNTNGTNREILRNIGFILSEQKIYTILGKNGSGK
                     STLIKSLTALLDNDIYRIDGNVFWYDENIFKMDVPRLLEIRKKEIKYVLQDLTKNYDP
                     LKKLKYYFDISGLDENTISFQLKSFLLPENKIISNLHPYEISGGMAQRLSLMFAISFN
                     PKLLILDEPTSAIDYANINLIKLKLIEFKKTGGSALIVTQDINFAKEISDEIAYLDDN
                     NLSEFIEAGSFFGDNTQNPFSGILKSFKDLQ"
     gene            1462..>2026
                     /locus_tag="VF870_14990"
     CDS             1462..>2026
                     /locus_tag="VF870_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9253549.1"
                     /translation="MKKVNSLLLLVIIISFFISCNNEKPELLNRAVIAISADLETINP
                     VYSFSVDEGVVDETLFLSLVQFEWNDESGDLTPKPMLASSWEWASDSSSIIFNLRDDV
                     VWSDGIKFNAEDVVYSFDIYSDPIVQSRYYGAFKNLYKDYEEHIDINKTFEILNPYKI
                     KINFIRGSASSLLDVVYPIIPKHIYEKL"
BASE COUNT          643 a          310 c          319 g          754 t
ORIGIN      
        1 aagatcatgt gaaatacata taattgtaag gttaaattct tcttttaatt tttttataag
       61 attgagtatg cccaattgag cttcaacatc ctgcgaggaa aatggttcgt ctaaaactaa
      121 aatttcaggt ccaactatta cgattcttgc gagagcgatg cgctgctttt cacctccact
      181 taattgtgat cctttgcttt ccttaagttg tgtattgaga ttaaaacgat cgaaaatttc
      241 atcaatttcg gaagtgtaca ctttattttg tttagacttt aattcaaaag cttcattaag
      301 aacatctttt acttttctaa aaggatttat caattcgcca tcattctgaa atagtatttg
      361 tacgggtttt tgtaacgatt tgctccaatc tcttttaaaa ttattctgaa taatacctga
      421 tgttggcgga aaaatacctg ctaatatttt tgccagcgtg gatttgccgc ttccggattg
      481 accggaaata ccgagtactt ttcctctttc aatttcaaaa gaaatatttt ttaatatttc
      541 aacagtttga gattgggaat agctattgct gcttcccgat aggccagttt ttttatcaac
      601 cgagtaagaa atattttcaa cttttagaat tatatctttc attgcaaatc tttaaacgat
      661 ttaagaattc cgctgaatgg attttgtgta ttatctccaa agaaagaacc agcttctata
      721 aattcactta aattattatc atcaagataa gctatctcgt cagaaatttc ttttgcaaaa
      781 ttaatatcct gagtaacaat aagtgcagaa ccacctgttt tcttaaactc aatcagttta
      841 agttttataa gatttatgtt tgcataatct attgctgatg ttggttcatc taaaattagg
      901 agttttggat tgaatgagat tgcaaacatt aaacttagtc gttgggccat tccgccgctt
      961 atctcgtaag gatgaagatt agaaataatt ttattctccg gcagtaaaaa actttttagc
     1021 tgaaatgaga tagtgttttc atccagacct gagatatcaa aatagtattt aagttttttc
     1081 agaggatcat agttctttgt taaatcctgt agtacatatt tgatttcttt tttacgtatt
     1141 tcaagtaatc ttggcacatc catcttaaaa atattttcat cataccaaaa aacattacca
     1201 tcaattctat aaatatcgtt atctaaaaga gcagtaagtg atttgatgag tgtagattta
     1261 ccgcttccat ttttgccaag tatagtataa atcttctgct cactcagtat aaaaccaatg
     1321 ttgcgcagaa tttctctgtt tgtaccattg gtgtttagaa taatttttct aagatcaact
     1381 ttaagcatta ttaaactttt gtatttgttg atcaaaataa taaattaacc aaagatttat
     1441 tagcatttaa ttgtactaat tatgaaaaaa gttaattccc ttcttttgct tgtcattatt
     1501 atcagttttt ttatttcatg taataatgaa aaaccggaac tgttaaacag ggcggtaatt
     1561 gcaatatctg ctgatcttga gactattaat cctgtttatt cattcagcgt tgatgaaggg
     1621 gtagtagatg aaactttatt cttgagttta gtacaatttg agtggaatga tgaatcaggt
     1681 gatttaacgc ccaaaccaat gcttgcatca agctgggaat gggcgtcaga ttcaagttct
     1741 attattttta atcttcgtga tgatgttgtt tggagtgatg gcatcaaatt taacgctgaa
     1801 gatgttgttt actcatttga tatttattcc gacccgattg tacaaagcag atattacggg
     1861 gcatttaaaa atttatataa agattatgaa gaacatatcg atataaataa aacatttgaa
     1921 atactgaatc cgtacaaaat aaaaataaat tttatacgag ggtctgcatc ttctttgttg
     1981 gatgttgttt atcccataat tcccaaacac atttatgaaa agcttg
//
LOCUS       DASWBY010000829         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_402517, whole genome shotgun sequence.
ACCESSION   DASWBY010000829 DASWBY010000000
VERSION     DASWBY010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402517"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VF870_14995"
     CDS             <1..521
                     /locus_tag="VF870_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253550.1"
                     /translation="SMQHIPATVSTYILVGGRTSTMRGTAVTYDAGETWTLIDTLGCF
                     LINFASDEEGWGSQWGNNVIYNYAGPRIISSVEEEIIDVVPTGYSLLQNYPNPFNPST
                     TFRYSIPTQSKVVIKVYDILGNEVATLMDEEKSVGTYELTWNASSLPSGVYFYQLKAG
                     SYVDTKKMILLK"
     gene            906..>1845
                     /locus_tag="VF870_15000"
     CDS             906..>1845
                     /locus_tag="VF870_15000"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX9253551.1"
                     /translation="MQFNRLTHQEGFPARNILAMHQDSKGFLWMAAPDRLIRFDGKRQ
                     KLFRYSSSNEKSIGSNTIRFIYEDKSGVLWFATAGGGLNKYNSSQENFIRYTHNRNDL
                     NSISSDDVSSICEDSYGNLWVGTIGGGLNKFDRKTEKFIAYKNDPSNIQSLSNNNVSV
                     ICLDSRNNLWVGTTGGGLNIFDIASKKFVHFIHNTSNPNSLSNNNVTGIVEDANGSLW
                     VSTMGGGLNNLTFLSDYQNLVFIHYKNNHNDQYSISSNDISTLYLDREKILWIGTWGG
                     GLNKMIQHPNDNSLVGFISYKHDPLDIFSLIDDNVNC"
BASE COUNT          612 a          300 c          347 g          586 t
ORIGIN      
        1 caagtatgca gcatatcccg gcaacagtct caacttatat tttagttggc ggtaggactt
       61 caacgatgcg aggcacagcg gtaacttacg atgccggaga aacgtggact cttattgata
      121 ccttaggttg ttttttaatt aacttcgcat ctgatgaaga aggctggggt tcacagtggg
      181 gaaacaatgt tatctataat tatgctggtc ctcgaattat tagttcagtt gaagaggaaa
      241 taattgatgt tgttccaact ggatattcac ttttgcaaaa ctatccaaac ccattcaatc
      301 caagcacaac tttcaggtat tcaattccaa cgcaatcaaa agttgttatt aaggtgtacg
      361 atattctcgg aaacgaagtt gccactttaa tggatgaaga aaaatctgtt ggaacttatg
      421 agttgacctg gaatgcatca tcgcttccaa gtggagtata tttctatcag ttaaaagctg
      481 gaagctatgt agacacaaag aaaatgattc ttttaaagta aaattaattc tgttgggctg
      541 ggaaaatcac ttcccagcca acattttgac gcagtattga taggtaaaaa gcgctttatt
      601 gtataaaaag aaagttctta atgatttaag tttgcggagc cacctaaaaa gccctgattt
      661 cagggctttt ttaatgtttt tttgcacacc ccgaacgaga aatagaaccg aataaaaatt
      721 tctaatattt gccattcctt tatctttttc ttgcacatct catcaattct taattatttt
      781 tctgttagtt aaatcggaat aaggctcaaa tgagtgtgca agttcggttt ttcatattca
      841 tcctaatcca gtttttattt ttgtccgacc agacatcttc ccaatcttat aatccaaacg
      901 gcaaaatgca attcaatcgt ttaacacatc aagaaggatt tccagctaga aatatattag
      961 caatgcatca ggatagtaag gggtttttgt ggatggccgc tcctgatagg cttataagat
     1021 ttgatggcaa gagacaaaaa ttatttagat actcctccag caatgaaaaa agtataggat
     1081 caaacactat aagatttatt tatgaagata agtcaggtgt attatggttc gcgacagctg
     1141 gcgggggact aaataaatat aattcatctc aagaaaattt tataagatat acacacaatc
     1201 gtaatgattt aaactcaata agctcagatg atgtttcctc gatttgtgaa gatagttacg
     1261 gtaatttgtg ggtgggaact ataggaggag gattgaataa atttgatcgt aaaactgaaa
     1321 agtttatagc ttataaaaat gatccttcaa atatccaaag tctgagtaat aataatgttt
     1381 cagttatctg tttggattcc aggaacaatc tttgggtagg aactacaggt ggaggattga
     1441 acatattcga tattgcttca aaaaagttcg tacactttat ccataatact tctaatccaa
     1501 atagcctaag taacaataat gtaaccggaa ttgtagaaga tgcaaatggt tctttgtggg
     1561 tatcaactat gggaggtggt ttaaataacc tgacgtttct cagcgattat caaaacctag
     1621 ttttcatcca ttacaaaaac aatcataatg atcaatacag tataagcagt aacgatatat
     1681 ccacacttta cttagataga gaaaaaattc tttggattgg aacgtggggt ggcgggctga
     1741 ataaaatgat tcagcaccca aatgataatt ccttagtagg atttatctct tataaacacg
     1801 accctcttga tattttttct ttgattgatg ataatgtgaa ttgca
//
LOCUS       DASWBY010000830         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_34974, whole genome shotgun sequence.
ACCESSION   DASWBY010000830 DASWBY010000000
VERSION     DASWBY010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34974"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /gene="mtnA"
                     /locus_tag="VF870_15005"
     CDS             <1..740
                     /gene="mtnA"
                     /locus_tag="VF870_15005"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX9253552.1"
                     /translation="NLFYALDRMKKVFQSITDFSNSYQILLNEAKAIFEFEEHCSGKI
                     AEHGFNIFTKKSNVLTHCNTGALATAGFGTAFAVIKKAFDEGYVNHVYADETRPLLQG
                     LRLTSYELEKNGIPFTVQTDSMAAVLMQQNKIDLVITGADRIALNGDSANKVGTYNLA
                     VLCNYHNIPFYIAAPSTTIDRKIPTGADIEIEYRNSSEILSINGNQITDPNIKTFSPA
                     FDVTPSHLIRGIITEEDLYFFPYNFIK"
     gene            737..1477
                     /gene="recO"
                     /locus_tag="VF870_15010"
     CDS             737..1477
                     /gene="recO"
                     /locus_tag="VF870_15010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX9253553.1"
                     /translation="MSDIIKTEAVVLSKLNYGDTSSIVSLYTESDGKLSAIIKGGRGP
                     KSKNGKIIDPLNHLQVIIYKKASRDIQILSDANLISHFVNIKEELDATKYGFAIIELV
                     KNLTADHEANTKLFKGLIKILNMINDKKEHPAFLYGRFLLFFLSELGYELLINKCTIC
                     GNDVVANKSLGFDFNTGFVCADCFVSHSGLENVSAELFDLIFCLKTNRLAEKFNVDLI
                     DKFNLLMERYLKFHVSDFKGIQSLQIYK"
     gene            1555..3042
                     /locus_tag="VF870_15015"
     CDS             1555..3042
                     /locus_tag="VF870_15015"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX9253554.1"
                     /translation="MKVKGILGAVALVAVGVLFGALLVSGFGLVRPGWADINLGATNP
                     PVNLDADATSFSKAFIEVAEKVTPAIVQINVVAERDNPHQDFFFFPFDDKLPKEQQGS
                     GSGIIISDDGYILTNNHVVEKATKVTVGLSDKRTFNAKVVGTDPLTDLAVIKIDAENL
                     TSAYLGDSDNLKVGQWVMAIGNPLSLSSTVTAGIVSAIGRGQLGLIRDSYGVENFIQT
                     DAVINPGNSGGAMVDLSGAVVGINSAIATQGTGTYIGYGFAIPINLAKTVAKEIIAYG
                     KVNRGYIGINIGEVDDALAKSVGLDKPRGIIIQGIVEGGAASETDLKSGDIILSIDGR
                     EVNQPNELQSYVASKTAGTTINLKIFRDGKEIDRKITLKARDEDTKTTPVKMKDEEGS
                     KSNSKSNTISFESIGMTVKNLSEKDKESLNVDSGILITKVEDFGKAADQRLGSGLIIV
                     EADKKKINDVAAFEKIIDAKKGKAVLLKVQDKDGNSRFVGLEIPE"
     gene            3125..>3222
                     /locus_tag="VF870_15020"
     CDS             3125..>3222
                     /locus_tag="VF870_15020"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX9253555.1"
                     /translation="MIRFLTAGESHGKNLTTIVDGFPSNLEITEDY"
BASE COUNT         1106 a          492 c          636 g          988 t
ORIGIN      
        1 taaatctttt ttatgctttg gacagaatga aaaaagtttt ccaatcaatt actgattttt
       61 ctaattctta tcaaatatta ttaaatgaag caaaagcaat ttttgaattt gaagaacact
      121 gttccggtaa aatagctgag catggtttta acatctttac aaaaaaatct aatgtgttaa
      181 cgcactgcaa cacaggtgca cttgcaacag caggatttgg aactgcattc gcagtaatta
      241 aaaaagcatt tgatgaagga tatgtaaatc acgtttacgc tgatgaaacg agacccttgc
      301 ttcaaggatt acgtttaact tcatacgaac ttgaaaaaaa tggtattcct tttacagttc
      361 aaacagattc tatggcagca gtattaatgc agcagaataa aattgattta gttattaccg
      421 gtgccgatag aattgcactt aatggtgata gtgcaaataa agtcggcaca tacaatcttg
      481 ctgtgctttg caactatcat aacatacctt tttacattgc cgctccatct acaactatag
      541 atagaaaaat accgacaggt gctgatattg aaattgaata tagaaatagt tcggaaatct
      601 tatctataaa cggaaatcaa atcactgatc cgaacatcaa aacgttttct cccgcttttg
      661 atgtaactcc atcgcattta ataagaggaa taatcacgga agaagacctt tatttcttcc
      721 cttataattt tataaaatga gcgatataat taaaacggaa gccgttgtgc tttcaaaact
      781 taattatgga gacacaagca gtattgtttc actgtacaca gaatcagatg gaaaactttc
      841 tgcaattatt aaaggcggac gcgggcctaa atcaaaaaac ggaaaaataa ttgatccgct
      901 gaatcatttg caggtgatta tctacaaaaa agcatcaaga gatattcaaa tactttccga
      961 tgcaaatctc atttcacatt ttgtaaatat taaagaagaa ctcgatgcca ctaaatatgg
     1021 atttgcaata atcgaacttg taaaaaatct tactgcggat cacgaagcaa atactaaatt
     1081 gttcaagggg ttaattaaaa tccttaatat gatcaacgat aagaaagagc atcctgcatt
     1141 tttgtacggc agatttttac ttttcttttt atcagaactt ggttatgaat tgttaattaa
     1201 taaatgcaca atctgtggga atgatgttgt tgccaataaa tctttaggtt ttgatttcaa
     1261 tacgggtttt gtctgcgcgg attgttttgt atcacattcg ggtctggaaa atgtatcagc
     1321 ggaacttttt gatttaatct tttgtctaaa aaccaataga ttagcagaga aatttaatgt
     1381 tgatctgata gacaagttta atttattgat ggagcgttac ctgaaatttc atgtaagtga
     1441 ttttaaagga atacaatcat tacaaattta taagtagagg aactatagat ataagcgata
     1501 ccgatgatca caaagttgat ctaattataa acaaaaaaat atcggagaat taaaatgaaa
     1561 gttaaaggca ttttgggagc agttgctctt gtagcagttg gtgtgctttt tggtgcttta
     1621 ctcgtttcag gttttggatt ggtaagaccg ggctgggcag atataaatct tggtgcaaca
     1681 aatccgccgg ttaatctgga tgctgacgct acatcgtttt cgaaagcatt tattgaagtt
     1741 gctgaaaaag taactccggc aatcgtacaa ataaatgtag ttgccgaaag agataatcct
     1801 catcaggatt ttttcttctt tccatttgat gacaaacttc ctaaagaaca gcaaggttcc
     1861 ggcagcggaa taataatttc ggatgatgga tatattttaa caaataatca cgttgttgaa
     1921 aaagcaacaa aagttactgt cggattatcg gacaaaagaa cttttaatgc aaaagttgtc
     1981 ggcactgatc cgttaactga tcttgctgtt attaaaattg atgctgaaaa ccttacttca
     2041 gcatatctgg gtgattctga taatctgaaa gtaggacagt gggtaatggc gattggaaat
     2101 ccgctttcac tttcttcaac agtaactgca ggaattgtaa gtgctattgg aagaggccag
     2161 ctgggtttaa ttcgtgatag ttacggtgtg gaaaatttta ttcagacaga tgcagtaatt
     2221 aatcctggta acagcggtgg tgcaatggta gatctttcgg gtgcagtagt cggaataaat
     2281 tcagccatag ctacacaggg taccgggaca tatattggtt acggttttgc aattccgatt
     2341 aatcttgcaa aaaccgttgc aaaagaaatt attgcatatg gaaaagttaa tcgtggttac
     2401 attggtatta acattggaga agtagatgat gctctcgcaa aatctgttgg attggataaa
     2461 ccacgcggca taattattca gggtatagta gaaggcggcg ctgcatcaga aacagacttg
     2521 aaatccggtg atataattct ttcaattgac gggcgtgaag ttaatcagcc aaatgaactg
     2581 caaagttatg tagcctcaaa aacagctggt acaacaatca atcttaaaat ctttagagat
     2641 ggaaaagaga tcgatcgcaa aataactctt aaagcacgtg atgaagacac taaaactact
     2701 ccggttaaga tgaaggatga ggaaggctca aaaagtaatt ccaaatctaa cacgatcagt
     2761 tttgaaagta ttggaatgac cgtgaaaaac ttaagtgaaa aagataaaga gagcctgaat
     2821 gttgatagtg gcatacttat cacaaaggtg gaagattttg gaaaggctgc tgatcagcgt
     2881 ttgggaagcg gattgataat tgttgaagct gataagaaaa aaattaacga tgtagctgct
     2941 tttgaaaaaa taattgatgc taaaaaaggc aaagcagttt tgttaaaggt tcaggataaa
     3001 gatggtaatt ccagatttgt tggattagaa attcctgaat agttaaattt taatatttta
     3061 tttcaacgtc ccggtaaatt tcgggacgtt tttttttata tttgccaaaa gtattggaga
     3121 gttaatgatc aggtttctta ccgcaggtga atctcacgga aaaaatttaa caaccattgt
     3181 tgatggattt ccttctaatc tcgaaataac cgaagactac tt
//
LOCUS       DASWBY010000831         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_34976, whole genome shotgun sequence.
ACCESSION   DASWBY010000831 DASWBY010000000
VERSION     DASWBY010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34976"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VF870_15025"
     CDS             <1..343
                     /locus_tag="VF870_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253556.1"
                     /translation="NSFYIHTELLFNSNGKTENVGLFADEAYQLGMLSAAKWSLYQEF
                     SYDITPLLRGSIFSIINPDDKSFVLVPSISYSIITNLDIYLIALLFNGDTLTEYGDYG
                     HYFYARLKWSF"
     gene            462..>1805
                     /locus_tag="VF870_15030"
     CDS             462..>1805
                     /locus_tag="VF870_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9253557.1"
                     /translation="MKNNNKLPLFEVVQIESIQDMLLKSAKTYANKLALEDLTNYPIS
                     KVNYSELNEYILKFGSSLQKLGLKRRDHIAVIGENRVQWAIAFLTSMMFDFVIIPVDK
                     NLNHADILNIIHESDASTLIFSGSFAELFKEKRDVLSKVKFYINMDLAKKENDFHSMA
                     EMIKDTEYITPELPKLNNEDIAEIVYTSGSLGRAKGVMLSQKNIASNLMAMRKMITIY
                     PEDRFLGVLPMHHTYQCTCGFLCVLYSGASLHFARSLKTITEDLIKVSPTVILGAPLL
                     YNKIYNKIMKTVSAKKITSTVFKAMLTVSRLTQKIGWRNSKKIIFGKIHKTLGGSMRL
                     LIAGGAAPDPKVSKFFREVGFNVVQGYGLTETSPILALNRLEYFKDESAGLPLDGVEI
                     KINQPDKNGIGEIYSKGQNSMLGYYNNKAATEETFEGEWFKTGDLGYFDEDGFLFI"
BASE COUNT          618 a          301 c          320 g          566 t
ORIGIN      
        1 aaattcattc tatatacata ccgaactact atttaacagt aacggaaaaa cagaaaatgt
       61 cggattattt gctgatgaag cttatcagct tggaatgtta agtgctgcaa agtggtcatt
      121 gtatcaggaa ttttcttatg acatcactcc tcttttgcgg ggttcaattt tttcaattat
      181 aaatccggat gataaatcat ttgtactcgt tccctcaata agctattcca taataacaaa
      241 tcttgatatt tatttaatcg cattattatt caacggagat actcttaccg aatatggcga
      301 ttacggccat tatttttacg caagactgaa gtggtcattt tagtcccacc aaattatttt
      361 tcttaaaatt cattttagcg gcacttcagt ttttacaatt ccatgacaac ttcattttga
      421 atcttttcta atttacagcc agatgattag agaggtgtga aatgaaaaac aataataaat
      481 tgccattatt tgaagttgtt caaatcgaat caattcagga tatgcttctc aaatccgcta
      541 aaacttatgc aaataaacta gcattagaag atcttacaaa ttatccaatc tccaaagtaa
      601 attactccga actcaatgaa tatattttga aattcgggag ctcacttcaa aaacttggtt
      661 taaaacgtag agatcacata gcagttattg gcgaaaatcg tgtccaatgg gcaatcgcat
      721 tcctaacttc aatgatgttt gactttgtaa taattcccgt tgataaaaat cttaatcacg
      781 ccgatattct taatatcata cacgagtctg atgcaagcac attaatattc tccggatcat
      841 tcgcagaatt atttaaagag aaaagggatg ttctgagtaa agttaaattc tacatcaata
      901 tggatttggc gaaaaaggaa aatgattttc attcaatggc agagatgata aaggataccg
      961 aatacatcac accggaatta ccgaaattaa acaatgagga tatagctgaa atagtttata
     1021 catcaggctc actaggcaga gctaagggtg taatgctaag ccagaagaat attgcttcta
     1081 acctaatggc tatgagaaaa atgataacaa tataccctga ggataggttt cttggtgttc
     1141 ttccaatgca tcatacctat caatgcacct gcggattttt atgtgttttg tattcagggg
     1201 catcgcttca ttttgcaaga tcattaaaga ctataacgga agatttgatt aaagttagtc
     1261 ctactgtaat tcttggggct cctttgctct acaataaaat ttataacaag attatgaaaa
     1321 cagtttctgc taaaaagatt acttctacag tttttaaagc gatgctgaca gtctcccgct
     1381 taactcaaaa aataggatgg agaaactcga aaaaaattat cttcggaaaa attcataaaa
     1441 cccttggggg atcaatgaga ttattaattg caggtggtgc cgcccctgat ccaaaagttt
     1501 ctaaattttt cagagaagtt ggtttcaatg tggttcaagg atatggcctg acagaaacct
     1561 cacctattct agctttaaac aggttagagt atttcaaaga tgaatccgca ggacttccgt
     1621 tagacggagt agaaataaaa attaatcagc cggataaaaa tggaattggt gaaatttatt
     1681 caaaaggtca gaacagtatg ctgggatatt ataataacaa ggcagctacg gaagaaactt
     1741 ttgaaggtga atggttcaaa accggagacc ttggatattt tgatgaggat ggatttctat
     1801 ttatc
//
LOCUS       DASWBY010000832         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_598758, whole genome shotgun sequence.
ACCESSION   DASWBY010000832 DASWBY010000000
VERSION     DASWBY010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598758"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VF870_15035"
     CDS             complement(<1..695)
                     /locus_tag="VF870_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX9253558.1"
                     /translation="MDLIFHIIPLFGVLALLYTFWRSRWISKQDAGSEKMQVIAGHIA
                     EGAMAFLKAEYRVLIFFVIVVAALLAFSGANSADSSPLVGLSFVVGAFCSALAGFIGM
                     RVATKANVRTTQAARTSIGKALEIAFAGGSVMGLGVVGLGVLGLGGLMIIYGDMYGMS
                     SQENLTKVITIITGFSFGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDHHLN
                     PATIADNVGDNVG"
     gene            complement(717..1313)
                     /gene="pth"
                     /locus_tag="VF870_15040"
     CDS             complement(717..1313)
                     /gene="pth"
                     /locus_tag="VF870_15040"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX9253559.1"
                     /translation="MRVIVGIGNPGSRYKNNRHNVGFQFLDFFCAEKKLTFKASKFDY
                     HFSEGEFSGAPFVLIKPDTYVNNSGLAVLDCLNHYKVDVENLLVVVDDINLSLSDIRI
                     RKNGGDGGHNGLSSIIYHLQSKSFSRLRIGIGNDFQSGNLADYVLSDFGKNDFLLLEK
                     SFNLSIQLVDSFILDGYNKLLGTFSRLKNLDKLNKEPE"
     gene            complement(1320..1961)
                     /locus_tag="VF870_15045"
     CDS             complement(1320..1961)
                     /locus_tag="VF870_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX9253560.1"
                     /translation="MEKVVIEAQKRDKIDKASRSALRKEGKVPAIFYSKHHEPLPVQF
                     SERAIHPLVYTSKTSLITLNVDGKEELDCIIKDVQFDPVSEKIVHIDLLGLKKGEKIQ
                     IEVPIQLVGNAIGIKEGGILQHTLHKIEIECLPIDIPEHLEIDVTELKLGHAIHAGDL
                     KYNNFIVLNSPESIIASVTHPKVEKTAEPSAGEGAEVAEPEVIAKGKPQDKEE"
     gene            complement(1979..2923)
                     /locus_tag="VF870_15050"
     CDS             complement(1979..2923)
                     /locus_tag="VF870_15050"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX9253561.1"
                     /translation="MASSGYMIFAGNSNLPLAEKIAESIGLQLGALELKRFSDGEIWV
                     KFGENIRGREVFLIQSTNPPAENLLELLIMIDAARRASAKRITAIIPYFGYARQDRKD
                     QPRVSITAKLMANLITEAGADRVMTMDLHASQIQGFFDLPVDHLYGSSIFLDHLSNLS
                     DNLSVVSPDVGGIKIARAYAKMLHSGLIVIDKRRPKQNLAEVMNIIGEVEGKDILLVD
                     DLIDTAGTFVGAAKALKERGAKKIYGAVTHPLLSGPAFERLNSSDIDALYVTDTINFD
                     ENACDRIVKISAAPLFGEAIQRTFKNESISSLFNIDKG"
BASE COUNT          978 a          627 c          438 g          973 t
ORIGIN      
        1 tcgccaacgt tatcgccaac attatccgca atagtagcag gattaagatg gtgatcttca
       61 ggtatcccag cttcaacttt acctactaag tctgcaccaa catcagcagc tttagtataa
      121 attcctccgc ccacacgtgc aaataaagca attgaagatg ctccaaatga aaagcctgtt
      181 ataatcgtaa ttactttagt aagattttct tgtgaagaca ttccatacat atctccgtaa
      241 ataatcatca atccgccaag ccctaaaact cccaaaccaa caacacctaa acccattaca
      301 gaaccaccgg caaaagctat ttccaaagct ttacctatac ttgttcttgc tgcctgagtt
      361 gttctaacat ttgcttttgt agcgactctc attcctataa acccggcaag cgcagaacaa
      421 aatgcaccaa caacaaaaga taatcctaca agaggagaag aatccgcaga gttagctcca
      481 ctgaatgcta ataaagcagc aacgacaatt acaaaaaata ttaaaactct atattcagct
      541 ttaagaaatg ccattgcacc ttctgcaatg tgcccagcaa taacctgcat tttttcactt
      601 ccggcatcct gtttagatat ccagcgtgaa cgccaaaaag tgtaaagtaa agcaagcact
      661 ccaaatagtg gaataatgtg aaatattaaa tccatgttta tctcctcaat tcgagtttat
      721 tctggttctt tatttaattt atcaagattt tttaatcggc tgaaggtacc aagcaattta
      781 ttataaccat ctaatataaa actatcaact agttgaatac ttagattaaa agatttttcc
      841 agcagcaaaa aatcgttttt accaaagtca gatagaacat aatcggcaag atttcctgac
      901 tgaaaatcat tccctatgcc tattcttaat ctggaaaaac ttttactttg caggtgatag
      961 atgattgaac ttaatccatt gtgcccgcca tcccctccgt tttttctaat ccggatatct
     1021 gacaagctta ggttaatatc atcaaccaca actaataaat tttcaacatc aactttatag
     1081 tgattaagac aatctaatac ggctaaacca ctattattaa cataagtatc aggtttaatc
     1141 agaacgaagg gagcacctga gaattcccct tcagaaaaat gataatcaaa ttttgaagct
     1201 ttgaaagtca gttttttttc tgcacaaaaa aaatcaagga actggaatcc aacattatgt
     1261 ctgttatttt tataacgact gcctggattt ccaattccaa caataacgcg cacttaaaac
     1321 tattcttctt tatcctgtgg tttaccttta gcaataactt caggttcagc aacttctgca
     1381 ccttctccgg cagatggttc agctgttttc tcaaccttag gatgagtaac tgatgctatt
     1441 attgattcgg gagaattaag tacgataaaa ttattatact tgagatcacc agcatgaata
     1501 gcatgcccga gttttagttc tgtaacatct atttccagat gttccggaat atctatcggt
     1561 aaacattcaa tttcaatttt atgaagagta tgttgtaaaa ttccaccttc tttaattcca
     1621 atagcattgc caactaattg aatcggaact tcaatttgga ttttctcacc ttttttaagt
     1681 cctagtaaat ctatatgaac aatcttttcc gaaacaggat caaattgcac atctttaatt
     1741 atgcaatcta attcttcttt tccatcaaca tttaaagtaa ttaaacttgt cttggatgta
     1801 tatacaagcg gatgaattgc tctttctgaa aactgcacag gaagaggttc gtgatgcttt
     1861 gaataaaata tagcaggaac ctttccttct ttacgtaaag cgcttcttga tgctttatca
     1921 attttatctc gcttctgtgc ttctattact actttctcca ttttaactcc gggtcttatt
     1981 aacctttatc tatattaaat aatgaactaa ttgattcgtt tttaaatgtt ctttgaattg
     2041 cctctccaaa taaaggagca gcagaaattt taacaattcg gtcacatgca ttttcatcaa
     2101 aattaattgt atctgtaaca taaagtgcat ctatatcact gctatttaat ctttcgaacg
     2161 caggcccgga aagcagtgga tgtgtaaccg caccatatat tttttttgcc cctctttctt
     2221 taagcgcttt agctgctcct acgaaagtac ctgctgtatc tatcaaatca tctacaagta
     2281 aaatatcttt tccttcaact tcgccgatta tattcattac ttcagcaaga ttttgttttg
     2341 gtcttctttt atcaataaca attaaaccag agtgcagcat tttagcataa gctctggcta
     2401 ttttgattcc accaacatca ggagaaacaa ctgaaagatt atcagaaaga ttacttaaat
     2461 gatccaagaa aatcgatgac ccataaagat gatcaacagg cagatcaaaa aatccttgaa
     2521 tctgtgatgc gtgcaaatcc atcgtcatca ctctatctgc accggcttca gtaattagat
     2581 tagccattaa ttttgcggtt atggaaacac gcggctgatc ttttctatcc tgtcttgcat
     2641 aaccaaaata agggataatc gcagttattc tttttgcaga ggctcttctg gctgcgtcta
     2701 tcattattaa aagttctaaa agattttcag caggaggatt agttgactga attaaaaaaa
     2761 cttcccttcc tctgatattc tccccaaact tcacccaaat ttcaccgtca ctaaaccttt
     2821 ttaattctag cgcccctaat tgcaaaccaa tagattctgc aattttttct gctaacggaa
     2881 gatttgaatt tccggcaaaa atcatgtaac cgcttgatgc cattaggtaa tgctctatta
     2941 aaattaaaaa taggtgttaa atataaccaa agaggtaagt tatgtcaata aaattaagta
     3001 tttacacggc tataag
//
LOCUS       DASWBY010000833         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_523087, whole genome shotgun sequence.
ACCESSION   DASWBY010000833 DASWBY010000000
VERSION     DASWBY010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523087"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VF870_15055"
     CDS             complement(<1..461)
                     /locus_tag="VF870_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253562.1"
                     /translation="MNNNIALDFSLFDGFPDMCFLVNENGDIRVLNKSSIQRLDLLNS
                     KPEIFFDYVELCDRIEAESLFKKSFFSGQSSDFETRLFIKKNFIDVKLIYVPFKSNGF
                     DEKLILIIARDISDEKKKETDLIRFFNVAENTVNPLQITDLNGKMIYVNKA"
     gene            610..1092
                     /locus_tag="VF870_15060"
     CDS             610..1092
                     /locus_tag="VF870_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253563.1"
                     /translation="MNEFLNILKYRLVGLNLILCVVFTILSFYSFETAAFLFVLFSNL
                     FDILGYHFSLIRRTTQLPEKIIIRSYRINQFLFDLLLLILIGFLFDWMAALSGWIMKL
                     FGLQDILYYLFLQKELPEKWTWMKWTPLGFFKGNLSKNEILIQAVTGIVIAVILLLLR
                     "
     gene            1105..>1704
                     /locus_tag="VF870_15065"
     CDS             1105..>1704
                     /locus_tag="VF870_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9253564.1"
                     /translation="MDKKLKIGLVQYSPVWENKSDSKEKIKNLFAQIGGLDLVIFPEM
                     TLTGFTMKSKEFAEDLTGESYQFFSSLAKQKKCALIYGLIEKGRKKNFNTLVHLNNQG
                     KILNSYRKIHPFSYSQEDEFYGKGKETVTTKVKGIKIGLSICYDLRFPELYRFYAKEK
                     ADMIIDIANWPDTRVEHWRALLKARAIENQCYAVGVNRVG"
BASE COUNT          577 a          235 c          276 g          616 t
ORIGIN      
        1 aaagctttgt taacataaat cattttacca ttaagatcgg ttatctgcag agggttaaca
       61 gtattttcgg caacattaaa aaaccttatc agatcagtct cttttttctt ctcatcactt
      121 atatctcttg cgatgatgag aattagtttt tcatcaaacc cattactttt aaaaggtacg
      181 tatattaatt taacatctat aaaattcttt tttataaata atctagtttc aaaatcactc
      241 gattgtccgc taaaaaatga ttttttaaac aatgattctg cctcaattct atcacaaagt
      301 tcaacataat caaaaaagat ttcaggtttg ctgttaagaa gatctaacct ttgtatagag
      361 gacttattta aaactcttat atccccgttt tcatttacca gaaaacacat atccggaaaa
      421 ccgtcaaata aggaaaaatc aagagctata ttattattca tcggatttaa atttgttcaa
      481 aaaattgttg ggctaaaata agtaatcctg aactatttta taagcctaaa ttgtgaccct
      541 atcgttataa gactttagta aatttgatgt ataagttctt tatggatttt gttttaatta
      601 tttttctata tgaatgagtt tttaaatatt cttaaataca gattagttgg gctgaacctg
      661 atactttgtg tcgtttttac aattttatct ttttattctt ttgaaactgc tgcatttttg
      721 ttcgtactgt ttagtaatct gtttgatata cttggttacc atttttcgtt aattcgaaga
      781 acaacacaac tgccagagaa aattattatt agatcatatc gaatcaatca atttttgttc
      841 gatctgcttt tgttaattct tatcggattt ttatttgatt ggatggctgc actttctggc
      901 tggattatga aattgtttgg attacaggat attctttatt atttattcct gcaaaaagaa
      961 ttacctgaaa aatggacgtg gatgaaatgg actccacttg gtttctttaa aggcaattta
     1021 tctaaaaatg aaattttaat acaagctgta actggaatag ttatagcagt tatactatta
     1081 ttattaaggt gatgagagat tcttatggat aaaaaattaa aaattggttt ggttcagtat
     1141 agtcctgttt gggaaaataa atctgacagc aaagagaaaa taaaaaattt atttgctcag
     1201 atcggcggat tagatttagt aatttttcct gagatgactt taacaggttt tacaatgaaa
     1261 tccaaagagt ttgcagagga tttaacagga gagagttatc agttcttttc ttcacttgca
     1321 aaacaaaaaa aatgtgcctt gatctatggc ttaattgaaa agggaaggaa gaaaaacttt
     1381 aatactttag ttcacttaaa caaccaggga aagattttaa actcatacag aaagattcat
     1441 cctttctctt actctcagga agatgaattt tatggtaaag gtaaagagac tgtaaccact
     1501 aaggttaaag gaataaaaat agggctttca atttgttatg atttacgttt tcctgaattg
     1561 tatagatttt atgctaaaga aaaagctgat atgattattg atattgcaaa ctggcccgat
     1621 acaagagttg aacattggcg ggcattactt aaagcacggg caattgaaaa ccaatgttat
     1681 gcggtcggag taaatagagt cggc
//
LOCUS       DASWBY010000834         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_148625, whole genome shotgun sequence.
ACCESSION   DASWBY010000834 DASWBY010000000
VERSION     DASWBY010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148625"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..397)
                     /locus_tag="VF870_15070"
     CDS             complement(29..397)
                     /locus_tag="VF870_15070"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9253565.1"
                     /translation="MISYSFSVSALIQSAPKNVYGIIADYKNGHPKILPKPPFVSLVV
                     EKGGVGEGSVLKVQMKVAGKLQTFKTVVTEPEPGHVLVETNDTGYITTFTVEPHDNGK
                     NSYVTFTTEIPGNSGLSKKR"
     gene            complement(493..1215)
                     /locus_tag="VF870_15075"
     CDS             complement(493..1215)
                     /locus_tag="VF870_15075"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9253566.1"
                     /translation="MEQTIQNVPTSMPRIGDKAPSFKAVTTQGDINFPEDYSGKWAIL
                     FSHPADFTPVCTSEFMTFATMEKQFNEANCQLVGLSVDGLYSHIAWLRTIKEKIEYKG
                     MKNVEVTFPLIEDITMNVAKTYGMLQPGESTTKAVRAVFFIDPKGIIRAIIYYPLSLG
                     RNFDELYRVIIALQAADEFSIATPADWRPGDDVIIPTAGSCGTAKNRMEGKEKDLTCH
                     DWFFCTKPIAKEEVLKRVLNKK"
     gene            complement(1453..>2271)
                     /locus_tag="VF870_15080"
     CDS             complement(1453..>2271)
                     /locus_tag="VF870_15080"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9253567.1"
                     /translation="ITCVMSMVISSKLSRESIYTLKLVLRNIGLKEGMETNVMESIYV
                     KNVYRKDYESINFADNFSQIVNRIIRGKETDFPVVNKNGIVTGMISIHDVKDYLYEKD
                     SLENILIAGDLANTYFETLCPDDSCQTALDKFRKFSFNGLPVVDNQETRKLLGIIWRK
                     DIQDAYDKEIEKRELTSNLASLITMKEDEPMVHFMEGYSIIEIAPPPFFIGKSIRQLN
                     IRSAYGVDVLSIKTKEKKGEKINAIPSPEYIITDQDTLIIAGEIKNITFIRNLT"
BASE COUNT          685 a          398 c          370 g          818 t
ORIGIN      
        1 ggaagaatcc tcttactgaa aaagaactct atcttttttt tgataaccca gagttaccag
       61 gaatttctgt tgtaaatgtt acgtaagagt tttttccatt atcatgcggt tcaactgtaa
      121 aagtggttat gtatccggta tcgttcgttt ctaccagaac atgaccaggt tcaggttcgg
      181 ttacaactgt tttgaacgtt tgaagttttc ctgccacctt catttgaact ttaagaacag
      241 agccttcgcc cacacctcct ttttctacaa caagcgatac aaacggcggt ttgggtaata
      301 tttttggatg accatttttg taatctgcaa ttataccata aacatttttg ggagccgatt
      361 gtattaatgc tgatacagaa aagctataac ttatcatgaa ttaatctttc aatcgttaaa
      421 gaatatatct ttaaaataaa aaaaaccgca aaagaatcct tctgcggttt taatcttttc
      481 ataattagtt aactactttt tattaagtac tcttttaagt acttcttcct tagcaattgg
      541 ctttgtacag aagaaccaat catgacaggt taagtctttt tctttgcctt ccatgcggtt
      601 ttttgctgtt ccgcaagagc cggctgtagg aataataaca tcatcgcccg gtctccaatc
      661 agcaggagtt gcaattgaaa attcatctgc agcttgaaga gcaattataa ctctgtaaag
      721 ttcatcaaag tttctaccca ggcttaaagg ataataaatt attgctctga ttatgccctt
      781 gggatcgata aagaaaactg cacgcacagc ttttgttgtg ctttcgcccg gctgaagcat
      841 gccatatgtt tttgcaacat tcattgtaat gtcttcaatc aaaggaaaag ttacttcaac
      901 atttttcatt cccttgtact caattttttc tttaatggtt cttaaccagg caatatggct
      961 gtaaagacca tcaacagaaa gaccaacaag ttggcaattt gcttcattga attgtttttc
     1021 catagttgca aaagtcataa actctgatgt acatacaggt gtgaaatctg cgggatgact
     1081 gaaaagaatt gcccatttac ctgaataatc ttcaggaaaa ttaatatcgc cttgtgttgt
     1141 tacggccttg aatgaaggcg ctttgtcgcc gatacgaggc attgatgtgg gtacgttctg
     1201 aattgtttgt tccattatag cacttcctaa attttaatta ttaattttgt tgattatatg
     1261 atgaagggaa atacacatag attctaaatc aattgaaatt ttattatgta acgttataaa
     1321 attatttctg aaaagctatt actgaataaa atatttgatt tgcgtaatgg caactattgt
     1381 gagatgacta atgacaatta taaaccccaa ttttgtaata agaatttatt gattcagttt
     1441 gtgaagtaag tgctaggtaa gatttctaat aaaagtgatg ttcttaattt ctccggcaat
     1501 aattaaggta tcttgatctg taattatata ttcaggactt ggtatggcgt taatcttttc
     1561 tccttttttc tctttggttt taatagataa aacatcaaca ccgtaagcgc ttctaatatt
     1621 taactgtcta attgatttac ctataaaaaa tgggggaggt gcgatttcaa taattgaata
     1681 accctccata aagtgaacca tcggttcgtc ttctttcatt gtaatcaggc ttgcaaggtt
     1741 tgatgttaat tcccttttct ctatttcttt atcataagca tcctgaatgt cttttctcca
     1801 gatgattcct aataattttc tcgtttcctg attatcaact acaggtaaac cattaaatga
     1861 aaatttcctg aacttatcca aagcagtttg gcagctatca tcgggacaaa gtgtttcgaa
     1921 atatgtgttt gctaaatctc cggcgataag aatattttct aaagaatctt tttcatataa
     1981 ataatctttc acgtcgtgaa tagaaatcat tccggttaca attccatttt tattaaccac
     2041 gggaaaatct gtttcttttc cccgtataat tctattaacg atttggctga agttatcagc
     2101 aaagttgatt gattcataat cttttctgta aacattttta acatagattg attccatcac
     2161 attggtttcc ataccttctt tcaaaccgat atttcttaga accagtttta aagtatagat
     2221 tgattctctt gaaagcttgg aagatatgac catgctcatt acacaagtta t
//
LOCUS       DASWBY010000835         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_749335, whole genome shotgun sequence.
ACCESSION   DASWBY010000835 DASWBY010000000
VERSION     DASWBY010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749335"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VF870_15085"
     CDS             <1..589
                     /locus_tag="VF870_15085"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX9253568.1"
                     /translation="KEEKYFEDFKEMISFIEEMAVHTEKIFSFDDPQTHILKMKPLEV
                     RCDELAYKITKRLNKTFITPFDREDIFALVKRLDDISDMLLGATVRVDTFHIDKKIDY
                     ADKIALIIREQVKELGVAIQDLKVKRVNEMKAVKALEVEADKIYQQAIKELFEKEKDA
                     IELIKKREILDILERTSDKCQSTANVILSIFIKNS"
     gene            640..>1512
                     /locus_tag="VF870_15090"
     CDS             640..>1512
                     /locus_tag="VF870_15090"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX9253569.1"
                     /translation="MTELIVIIFLALAFDFINGFHDAANSIATVVSTRVLTPRVAVVW
                     AAFFNFIAFLAFGLHVAHTIGKGIVRTDIVDLTVIGSGLLAAIIWNLLTWWWGIPSSS
                     SHTMVGGFVGAAISKAGIGAIFVPEVLKITAFIFLAPLIGIVISVFFSIIVLHVVKDS
                     SPFKVDKYFRKLQLVSSAAYSLGHGSNDAQKMMGIIFVALISTGYLTANDEIPLWVVL
                     SCHAAIALGTLSGGWRIVKTMGQRITKLRPFEGFSAETAGAITLFGTAIAGIPVSTTH
                     TITGSIIGAGITKRM"
BASE COUNT          489 a          225 c          297 g          501 t
ORIGIN      
        1 taaagaagaa aagtatttcg aagactttaa agagatgata tctttcatcg aagagatggc
       61 ggttcatact gaaaagatat tttcattcga tgatccgcaa acccatatcc taaaaatgaa
      121 acctttagaa gttagatgtg atgagcttgc ttataaaatt actaaacgat tgaataaaac
      181 ttttatcact ccatttgatc gcgaagatat tttcgctcta gtaaaaaggc tggatgacat
      241 aagcgatatg cttcttgggg caactgttag agtagatact ttccatatag ataaaaaaat
      301 tgattacgca gataaaattg cactgattat ccgcgaacaa gtaaaggaac ttggtgtagc
      361 tattcaggat cttaaagtta aaagagtgaa tgaaatgaag gcagttaaag ctcttgaagt
      421 tgaagctgat aaaatttatc agcaagcgat aaaagaattg tttgagaaag aaaaagatgc
      481 aattgaactt ataaagaaac gggaaatatt agatattctt gaaagaacta gtgataagtg
      541 tcagtcaaca gcaaatgtta ttctttcaat ttttattaag aactcatagt aagaaaaaca
      601 ttattataca atacactaca acactttgat atttatatga tgactgaact tattgtaata
      661 atttttttag cattagcatt tgattttata aatggatttc acgatgctgc taattcaata
      721 gccaccgtag tttcaacaag agtgttaact ccacgggtgg cagtagtttg ggccgcattt
      781 tttaatttca tagcgttttt agcttttggt ttgcatgttg cgcatactat tggaaaaggc
      841 attgtaagaa ctgacatagt agatttgaca gtaataggta gtgggttact tgctgcaatt
      901 atttggaatc ttttaacatg gtggtgggga atcccttcta gttcatcaca cactatggtt
      961 ggtggatttg ttggtgctgc aatttcgaag gctggaattg gagctatttt tgttccagaa
     1021 gttttaaaaa taactgcatt tatttttctt gccccgttaa ttggaatcgt tatttctgta
     1081 ttcttttcaa ttatcgtttt gcatgtagtt aaagattcta gcccatttaa agttgataaa
     1141 tattttcgaa aacttcaact cgtctcttca gctgcataca gtttgggtca cggttctaat
     1201 gatgcccaaa aaatgatggg aattatcttt gttgcactaa tttctactgg atatttaaca
     1261 gccaatgatg aaatcccttt gtgggttgtg ctttcctgtc atgctgcaat tgcacttggt
     1321 acactttctg gtggatggag aattgttaaa acaatgggac aaagaattac caagcttcgc
     1381 ccatttgaag gatttagcgc tgaaactgct ggtgcaataa ctttatttgg aactgcaatc
     1441 gctggtattc ctgtgagtac aacccacaca attacaggaa gtatcattgg agcaggaatt
     1501 actaaaagaa tg
//
LOCUS       DASWBY010000836         5000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_749429, whole genome shotgun sequence.
ACCESSION   DASWBY010000836 DASWBY010000000
VERSION     DASWBY010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749429"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1689
                     /locus_tag="VF870_15095"
     CDS             34..1689
                     /locus_tag="VF870_15095"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9253570.1"
                     /translation="MKQIITQIKENEIFFKREDFQKNLLRKLEATKAKVIEGGGKRSI
                     EKHKAKGKLTARERVNLLVDDPKRFFELSTFAAYGMYEEYGGAPSSGTIYGIGKIHNR
                     DCVIVANDATVKAGAWFPITAKKNLRAQEIAMENHIPIIYLVDSAGVFLPLQDDIFPD
                     KEHFGRIFRNNAKMSSMGIPQIAAIMGPCVAGGAYLPIMSDEALIVEGEGSVFLAGAH
                     LVRAAIGEVIDNEKLGGAKVQSNISGVTDYVMENDEECITQIRSLVEKFGKNPTSGFD
                     RIESRQPKYPIKEILGVLPEDTLKPYDTYELLSRIVDNSEIDEYKAGYGKTIITAYAR
                     IDGWAVGIVANQRSVVKTELGEMQVGGVIYSDSADKATRFIMNCNQKKIPLLFLQDVT
                     GFMVGSKAEHGGIIKDGAKMVNAVANSVVPKLTIITGNSFGAGNYAMCGKAYDPRFIF
                     AYPNAKISVMGGNQASSVLLDIKLKQEEKSGHKFSDADKKKLLDDILKSYDEKSTPLY
                     AAARLWVDEIIDPSQTRKWISICLEVANNNPEMPKFNPGVIQT"
     gene            1770..2414
                     /locus_tag="VF870_15100"
     CDS             1770..2414
                     /locus_tag="VF870_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253571.1"
                     /translation="MKFLIIITLLITFQTIQAQQSKLSKEVNYISSYIATDHFSKLKN
                     DIGDVSAADSIFVEAINFTHGNVSEALLALMLATVPYREVPVQIPLINSILNYPLTSA
                     DEETFLKKNNNLPRNLFFDTPQNNYGDKDKLAHFFGSAFLSYESNFFDLGKLIGYFVE
                     AFEESFKVQSSVDIRDLDVNDYGRLFGDLLKENNNLLPSQIFLIRSIRFLRVTL"
     gene            2411..3766
                     /locus_tag="VF870_15105"
     CDS             2411..3766
                     /locus_tag="VF870_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9253572.1"
                     /translation="MKSVLVIDDELQICESINMILEYEGYAVDFTTSANEGLEKFSSQ
                     EFSAVLLDIQMPEMNGFEVLKKLKVQKPAVSVIIISAHGSVENAIKATRLGAFDFLEK
                     PIDRDKLLISVRNATETASLKEENEVIKKVWIGDGEILGKSKAIQNILEMIDKVAPLD
                     TRVLITGENGTGKELVARAIHRKSERKDKAFVEVNCAAIPNELIESELFGHEKGSFTG
                     AVQQRIGRFELAHKGTIFLDEVGDMSQQAQAKVLRAIEDGKIERVGGGKKIDVDVRLI
                     AATNKNLKEEIEKGNFREDLFHRLNVIPIHVPPLRERSEDIPILVERFTNDISLKHKK
                     PLTKFSDEAIQILKNQSWTGNVRELRNIIERIIIIVDKKEILAKDIDFMFAGNQKSVD
                     ELVDTSNSFQEFKEKAERVFILKQLRSNDWNISKTAEILEIQRSHLYNKMKKYGIEKE
                     E"
     gene            3766..4110
                     /locus_tag="VF870_15110"
     CDS             3766..4110
                     /locus_tag="VF870_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX9253573.1"
                     /translation="MSTIFSKIISKEIPANIVYETDDILAFKDINPQAPVHVLIIPKI
                     EIPKVTNLNGIEHASLLGKMIDAANHIAKDMKIDKDGFRLVFNCGDNGGQEVYHLHLH
                     LLGGRKMNWPPG"
     gene            complement(4193..>5000)
                     /locus_tag="VF870_15115"
     CDS             complement(4193..>5000)
                     /locus_tag="VF870_15115"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253574.1"
                     /translation="KEIWDNISLPISIGIGSNKTIAKIGSDCMKPKGITYIIPGMEKE
                     FLSPMPVETIPGVGKVMKQSLNSRGIYRIGDISKLSSDYFGTAFGKYGIDLWRKANGE
                     GTEYLTIQRTRKSISRETTFGSDVTSEDEIKNTLFHLTGKVAQSLRKKGWEASTIDIK
                     LRYTDFQTLTRAKTIKPADDDKVIFETAWDLMKKARTRRVGVRLIGVGVTNFSPLNEQ
                     EFLFEDYEVKRKKMLRAVTRIRDKFGYESLMFGNTGEDKKLKGMENYFQV"
BASE COUNT         1683 a          795 c          947 g         1575 t
ORIGIN      
        1 taattgcaag ggatttgttg aaagattaat tatatgaagc aaatcattac tcaaataaaa
       61 gagaatgaaa tattttttaa acgagaagat tttcaaaaga atttacttcg caaacttgaa
      121 gcgactaaag caaaagtaat agaaggcgga ggaaaaagat ccattgagaa acataaagcc
      181 aaaggaaagc taactgcacg tgaaagagta aatcttcttg ttgatgatcc aaaaaggttt
      241 tttgagttaa gcacttttgc agcttacgga atgtatgaag aatatggcgg tgcgccatct
      301 tccggaacaa tttatgggat tgggaaaatt cataatcgtg actgcgttat tgttgcgaat
      361 gacgcaacag taaaagccgg tgcctggttc cctatcacag ccaaaaagaa tttgcgtgct
      421 caggaaattg caatggaaaa tcatatccca attatttatt tggtagatag tgcaggagtt
      481 tttcttccat tgcaggatga tatttttccc gacaaagaac attttggaag aatctttcgc
      541 aataatgcta aaatgtcttc aatgggcata ccacagatcg ccgcgattat gggaccttgt
      601 gttgcaggtg gtgcatatct tccaataatg agtgatgagg ctttaattgt cgaaggagaa
      661 ggatcagtct ttcttgccgg tgctcatctg gttcgcgcag caattggcga agttattgat
      721 aatgaaaaac tgggtggtgc aaaagttcaa tcaaacattt ctggcgttac agattatgta
      781 atggaaaatg atgaagaatg tattacgcaa ataagaagtt tggttgaaaa gtttggaaag
      841 aatccgactt ccggcttcga taggattgaa agtagacagc caaaatatcc aattaaagaa
      901 attcttgggg ttttaccgga agacacttta aaaccctacg atacttatga gttactttca
      961 cgcattgttg ataattctga aattgatgag tacaaagccg gttatggaaa gactatcatt
     1021 acagcttatg cacgaattga tggctgggca gttggaattg ttgctaatca aagaagtgtt
     1081 gtaaaaactg agcttggaga aatgcaggtt ggcggagtaa tttattctga ttctgctgat
     1141 aaagcaacaa gatttattat gaattgtaat cagaaaaaaa ttcctttgct ctttttacaa
     1201 gatgtaaccg gatttatggt tggcagtaaa gccgaacacg gcggcataat taaagatggg
     1261 gcaaagatgg tgaatgctgt tgcaaattct gtagttccta aacttacaat tattacaggc
     1321 aacagttttg gtgccggcaa ttacgcgatg tgcggcaaag cttatgatcc acgatttatt
     1381 tttgcttatc ctaatgcaaa gatttctgtt atgggtggaa atcaagccag cagtgttttg
     1441 ttagatataa aacttaagca ggaagaaaaa agcggacata aatttagtga tgccgataaa
     1501 aagaaattgc tggatgatat tctaaaatct tatgatgaaa agagcactcc actttatgcc
     1561 gcggcaaggt tatgggttga tgaaataatt gatccgtcac aaactcggaa atggatttct
     1621 atatgccttg aagttgcaaa taataatccg gaaatgccta agtttaatcc tggtgtaatt
     1681 caaacataaa atatatatca gtatttaacc tttaaaatta cttagtttca atttgtatct
     1741 ttttcttgcc taaatgtttt agtttcaaga tgaaattctt aataattatt acactactaa
     1801 ttacgtttca aactatccaa gctcagcaaa gcaaattatc taaagaagta aattacattt
     1861 caagttatat tgccaccgat catttttcta agcttaagaa tgatattggg gatgtatctg
     1921 ctgccgattc aatttttgtt gaagctataa attttaccca tggaaatgtt tcggaagcac
     1981 tgcttgcatt aatgttagca actgttcctt atcgggaggt tcctgtccag attccattaa
     2041 taaattccat attaaactat cctttaacat cagccgatga agaaactttt ttgaagaaga
     2101 ataataatct tcccagaaac ttattttttg atacaccaca aaataattat ggagacaagg
     2161 ataaacttgc tcattttttc ggcagtgcgt ttttatccta tgaatcaaac ttctttgact
     2221 tgggaaagct aattggttat tttgtagaag catttgaaga aagttttaaa gtgcagtcaa
     2281 gtgttgatat acgcgacctt gatgtaaatg attatggaag attgttcgga gatcttctta
     2341 aagaaaacaa taatttatta ccatcacaaa tatttttaat acgttccatt agatttttaa
     2401 gagttacatt atgaaatcag ttttagttat tgatgatgaa cttcaaattt gcgaaagcat
     2461 aaatatgatc ctcgaatacg agggttatgc agttgatttt acaacaagtg caaatgaagg
     2521 tttagaaaaa ttttcatccc aggagttttc tgctgtctta cttgatattc agatgccgga
     2581 aatgaatgga tttgaggtac tgaaaaaatt aaaagtgcag aagccagctg tatctgtgat
     2641 tatcatttct gcgcacggca gtgttgaaaa tgctattaaa gcaacccgac tgggtgcatt
     2701 tgattttctt gaaaagccga ttgaccgtga caaactttta attagcgttc gaaatgcaac
     2761 tgagacagca tcactaaaag aagaaaatga agtaattaaa aaagtttgga tcggtgatgg
     2821 tgaaatcctt ggaaagagca aagctattca aaatattttg gagatgattg ataaagtggc
     2881 accattggat acccgtgttt taataacggg tgaaaatgga acaggaaaag aattggttgc
     2941 acgagcgatt catagaaaaa gtgagcgaaa agataaagca tttgttgaag ttaactgtgc
     3001 agcaattcca aacgaattaa ttgagtcgga attatttggg catgaaaagg gatcttttac
     3061 gggagcggta caacaacgaa ttggcagatt tgaacttgca cataaaggca caatattttt
     3121 ggatgaagtc ggtgatatgt cgcagcaggc gcaggcaaaa gttcttaggg ccatcgaaga
     3181 tggtaaaatt gaacgcgttg gcggaggtaa aaagattgat gtggatgtaa ggcttattgc
     3241 cgcgacaaat aaaaatctta aagaagaaat tgaaaaagga aattttagag aagatttgtt
     3301 tcacagatta aatgtaattc cgatacacgt tcccccacta cgtgaaagat ctgaagatat
     3361 accaattctt gttgaacgtt tcacaaatga tatttcgttg aagcataaaa aacctttaac
     3421 aaaattttca gatgaagcaa ttcaaattct caaaaatcaa tcctggaccg ggaatgtaag
     3481 agagttaaga aatattattg agcgaattat tatcatagtt gataaaaaag aaattttggc
     3541 aaaagatatc gattttatgt ttgctggtaa tcagaaatca gttgatgaac ttgttgatac
     3601 cagtaattca ttccaggaat tcaaagaaaa agcagagcgg gttttcattc tcaagcagct
     3661 tcgttctaat gattggaaca taagcaaaac cgctgagatt cttgagattc aacgcagtca
     3721 tctttataac aaaatgaaaa aatatgggat agaaaaggag gaataatgtc aactatcttc
     3781 tctaaaatta tcagtaaaga aattccagct aatattgttt atgagactga tgatattctt
     3841 gcatttaaag atattaatcc tcaagcaccg gttcacgttt taataattcc caaaattgaa
     3901 attcctaaag tgactaattt aaatggaatt gaacacgctt cgcttttagg taaaatgatt
     3961 gatgcagcaa atcatatagc aaaagatatg aaaatagata aagacggatt tagattagtt
     4021 tttaattgtg gtgataacgg cgggcaggaa gtttatcact tgcacctgca cttacttggc
     4081 ggcagaaaga tgaattggcc tccgggataa caagaattaa tcatcctgga tttatttcag
     4141 aatctttata gattccgaaa caagttcgga atgactgcac ttatatcgta catcaaacct
     4201 gaaaataatt ttccattcct ttcaattttt tatcttcacc tgtatttcca aacattaaag
     4261 attcatatcc aaatttatca cgaattctgg ttactgcccg cagcattttt tttcttttca
     4321 cttcataatc ttcaaacaaa aattcttgtt catttaacgg agaaaaattt gtgaccccaa
     4381 ctccaattaa cctgacgcca actctacgag ttcttgcctt tttcatcaaa tcccaggctg
     4441 tttcaaaaat aactttatca tcatcagcag gtttaattgt ttttgcacga gttaatgttt
     4501 gaaaatctgt ataacgaagt tttatatcaa ttgtagatgc ctcccatcct tttttacgta
     4561 atgattgagc tacttttcct gttagatgaa acaaagtgtt tttaatctca tcctcactag
     4621 taacatcact tccaaaagtt gtttctcgcg aaatactttt tctcgttcgc tgaattgtta
     4681 aatattctgt tccttcacca ttagcttttc gccagagatc tattccgtat tttccaaaag
     4741 ctgtcccaaa ataatctgat gaaagttttg agatatcacc aattctataa attccacgtg
     4801 agtttaaact ttgcttcatc actttaccaa cacccggaat agtttccacc ggcattggag
     4861 acaaaaattc cttttccatt cccggaataa tataagttat acctttaggc ttcatacaat
     4921 ctgagcctat ttttgctatg gttttgttgc ttccaattcc tatagaaata ggtaaactta
     4981 tattatccca aatttctttt
//
LOCUS       DASWBY010000837         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_523407, whole genome shotgun sequence.
ACCESSION   DASWBY010000837 DASWBY010000000
VERSION     DASWBY010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523407"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..573)
                     /locus_tag="VF870_15120"
     CDS             complement(67..573)
                     /locus_tag="VF870_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEX9253575.1"
                     /translation="MISAKMQKALNTHLNEEFYSSYLYLSMAAYFEAKNLKGFANWMR
                     IQSTEEQMHGMKFFNFILQKGGKVSLTDIGAPKTEWKSISEVFSDTLKHEQKISGLIN
                     KLVEVAMTEKDYATNTFLQWFVTEQVEEEANVEEIIQKIEMIGDNKSGLYMLDNELVS
                     RAAAPASA"
     gene            784..>1622
                     /locus_tag="VF870_15125"
     CDS             784..>1622
                     /locus_tag="VF870_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX9253576.1"
                     /translation="MAVKVAINGFGRIGRLVLRAMADKGVLGKEVDLVALVDITNDAK
                     YFAYQLKYDSVHGIFKGEITSKKSKADLEHDDVLVINGEETLCVLATKDPSQLPWKEL
                     GVDYVIESTGLFTDSEKAKGHIAAGAKKVIISAPGKGDVKTVVMGVNDNELKPEHTII
                     SNASCTTNCLAPVVHVLLKEGIGVEVGIMTTIHSYTATQKTVDGPSKKDWRGGRAAAI
                     NIIPSSTGAAKAVGEVLPSVKGKLTGMSFRVPTANVSAVDLTFRAAKDTSIKEIDELL
                     KKAS"
BASE COUNT          536 a          261 c          285 g          540 t
ORIGIN      
        1 agaaattatt aataaaaaaa tcctctcaaa tttgagagga ttttttgttt taaatcttaa
       61 aaaattttat gcactggcag gtgctgcagc ccttgatact aattcattat caagcatata
      121 aagaccgctt ttattatcac caatcatttc aatcttttga atgatttctt cgacattcgc
      181 ttcttcttca acttgctcag taacaaacca ttgcaagaaa gtatttgttg catagtcttt
      241 ttcagtcatc gcaacttcaa caagtttatt tatcaaacct gaaatttttt gttcgtgttt
      301 taatgtatct gaaaaaactt cagaaataga tttccattct gtttttggtg caccaatatc
      361 agttaaactt actttgccgc ctttttgtaa aatgaagtta aaaaatttca taccatgcat
      421 ttgctcttct gtggattgaa ttctcatcca gtttgcaaat ccttttaaat tttttgcctc
      481 aaaataagct gccattgata ggtataaata agaagaataa aactcttcat taagatgtgt
      541 gtttaaagct ttttgcattt ttgccgatat cataaataac ctcttatgtt ttaaatctgt
      601 tatagttctt aactcaaata taatttattt agttcaaaat cacctctttt aagtgtgaat
      661 atttttaacg gcttgtgttg gagtacacac gattcgggtt gaattgaatt gataataaaa
      721 agctctttga ctaattttgg atgggcttat tataaagatt aaattcatta aaggagttat
      781 aaaatggcag ttaaagtagc tattaacggg tttggaagaa ttggaagact cgttttacgt
      841 gcaatggcag ataaaggcgt tttgggtaaa gaggttgatc ttgttgcctt agtggatatt
      901 actaatgacg caaaatattt tgcttaccaa ttaaagtatg attccgttca cggaattttc
      961 aaaggtgaaa ttacatcaaa gaaaagtaaa gctgatcttg aacacgatga tgttcttgta
     1021 ataaatggcg aagaaacttt atgtgttctt gctacaaaag atccgtctca attaccatgg
     1081 aaagaacttg gagttgatta cgtaattgaa tcaaccggac tctttacaga ttctgaaaaa
     1141 gcaaaaggtc acattgctgc aggcgcaaag aaggtcatca tttcggctcc tggtaaagga
     1201 gatgttaaaa cagtcgttat gggcgttaac gataacgaat taaaaccaga gcatactatt
     1261 atttctaatg cttcatgtac aactaactgt ttagcacctg ttgttcacgt tttattaaaa
     1321 gagggaatag gagttgaagt tggaataatg acaacaattc actcatatac cgcaacacaa
     1381 aaaacagtag atggtccttc caaaaaagat tggagaggcg gaagagctgc tgcaataaat
     1441 attattccat caagcacagg cgcagcaaaa gctgttggtg aagttcttcc ttcggtaaaa
     1501 ggaaaattaa ccggaatgtc attccgtgtc cccaccgcaa atgtttcagc tgttgattta
     1561 acatttcgtg cagcaaaaga tacttctata aaagaaattg atgagctgct caagaaagct
     1621 tc
//
LOCUS       DASWBY010000838         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_749720, whole genome shotgun sequence.
ACCESSION   DASWBY010000838 DASWBY010000000
VERSION     DASWBY010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749720"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VF870_15130"
     CDS             complement(<1..401)
                     /locus_tag="VF870_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253577.1"
                     /translation="MFKKIAEKFKGDRKYFSIVFFILIILAGSGIVTESILNKTQKNW
                     NNILTEKISDLEVSIKSDFNKKQNDLEEKLNLARRNLRKSLAPENESYKEIVKIINDE
                     VFVDYSIEIFAPNGKLIAWNQIIAIDQNELFP"
     gene            complement(401..880)
                     /locus_tag="VF870_15135"
     CDS             complement(401..880)
                     /locus_tag="VF870_15135"
                     /inference="COORDINATES: protein motif:HMM:NF027349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HEX9253578.1"
                     /translation="MTDMGSRQTFLNMHKIFYILIIFLPLAVLFSQTSNLTGKTETKT
                     SSQQEESIFKEIESAIKEANVEQISKYFGQQTYFSFSNGTNGYYSSNQAFYVVEDFFK
                     LYRVTSFKLDHVKTDNNHSYATGKYNYDNRGQRSTAQVYISLKKIGTNWIITQFTIN"
     gene            complement(921..1736)
                     /locus_tag="VF870_15140"
     CDS             complement(921..1736)
                     /locus_tag="VF870_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX9253579.1"
                     /translation="MKKLSIFAILILTTLILSSCDVNDTDYYDYTPPSPPTGIQVLNG
                     DNRVDIFWNRNRESDVAGYNVYYNYTYEGKYTLIGSTSGTQYADIEAVNGNKYYYAVA
                     AYDFNGNESELSYDVIYSTPRPEGFNQPIYDAQRFPEYAGYSFQNYSVVAFNDNAADF
                     YFDIYEGVPYLNLWEVPAPTYIQDMGLTNDIYDIPYAPASGWVFQDYIRAYVGHTYVI
                     RTIENQFAKIRIKQINSDGIEFDWAFQTVPGEPQLKPIVRSGERNLDKLQLAR"
     gene            complement(1901..>2087)
                     /locus_tag="VF870_15145"
     CDS             complement(1901..>2087)
                     /locus_tag="VF870_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9253580.1"
                     /translation="IKEGIEANLTILDIDTVWTVDKKKFLSKSSNSPFDKRLLTGKSV
                     GVINKGMMFIEGSFSEI"
BASE COUNT          682 a          353 c          307 g          745 t
ORIGIN      
        1 ggaaataact cattttggtc gattgcaatt atctgattcc aggcaataag tttaccattt
       61 ggggcaaaaa tctctattga ataatcaacg aaaacttcat cgtttataat tttaactatc
      121 tctttataag attcattctc cggtgctagt gattttctaa gattacggcg ggccagattt
      181 aatttttctt caagatcatt ttgcttttta ttaaaatcag actttattga aacttcaagg
      241 tcagaaattt tttctgttaa aatgttgttc caattttttt gtgttttatt taaaattgat
      301 tcagttacaa tacctgaacc cgcaagtatt attaaaataa aaaagacgat agagaaatat
      361 tttctatcgc ctttaaactt ttcagctatc ttcttaaaca tcagttaata gtaaattgag
      421 ttataatcca attagtaccg attttcttta atgaaatata aacctgtgca gtgcttctct
      481 gtcctctatt atcataatta tattttccgg ttgcgtagga atggttgtta tcagttttaa
      541 catgatcgag tttaaacgag gtaactctat ataatttgaa aaagtcttct acaacataaa
      601 aggcctgatt gctgctgtaa tatccattag taccgttcga aaaactaaaa taggtttgct
      661 gaccaaaata ctttgaaatt tgttcgacat ttgcttcttt aattgcagat tcaatttcct
      721 taaaaattga ctcttcctgc tgactactag tttttgtttc ggtttttccg gtaagattgg
      781 atgtttgaga aaatagtacc gcaagcggta aaaatattat aagtatgtaa aatattttat
      841 gcatatttaa aaaggtttgt cgtgaaccca tatctgtcat tttcaaatac aagtccacga
      901 caaaagtaat aaattcttaa ttatctcgcc aattgtaatt tatctaagtt acgctcacca
      961 cttcttacaa taggctttaa ttgtggttca cccggaacag tttggaaagc ccaatcaaat
     1021 tcaattccat cagaattaat ttgtttaatt ctaattttag caaattgatt ttcaattgta
     1081 cgtataacat acgtatgccc aacatatgct ctaatataat cctggaatac ccatcccgat
     1141 gctggagcat atggaatatc ataaatatca ttagtaagac ccatatcctg aatataagta
     1201 ggagctggta cttcccatag atttaaataa ggaacccctt cataaatatc aaaataaaaa
     1261 tctgctgcat tgtcattaaa tgctacaacc gaataatttt gaaatgaata accagcatat
     1321 tcgggaaatc tttgagcatc ataaatgggc tgattaaacc cttccggtct tggagttgag
     1381 taaattacat cgtagctaag ttcactttcg ttgccattaa aatcataagc tgcaactgca
     1441 taataatatt tattgccatt tacggcttca atatcagcat attgagttcc tgatgttgaa
     1501 ccaattaagg tatattttcc ttcgtaagtg taattataat aaacattgta tccggcaaca
     1561 tcgctttccc tgtttcgatt ccaaaaaata tccactctat tatcaccgtt gagcacttgt
     1621 attccggttg gaggcgatgg cggtgtatag tcgtagtaat ctgtatcatt tacatcacaa
     1681 gaactcaaaa ttagagttgt taggattaaa attgcgaaga ttgatagctt tttcatttta
     1741 ttcaccttta actgtgtttt cattgtcaca gattataacc caaaataaat gccatataaa
     1801 aatgcggttt attaacatta aacgcagaat atttagactg gttgtacagt taattgaaca
     1861 atgattggac aattatttag ttcagtagag gctgaatcaa ttaaatttca gagaaactac
     1921 cttcaataaa catcattcct ttattaataa ctccaactga tttacccgtt agtaatcgtt
     1981 tatcaaatgg cgaatttgaa gatttggaca agaatttttt cttatcaact gtccagactg
     2041 tatcaatatc caaaattgtc agattagctt caattccctc ttttata
//
LOCUS       DASWBY010000839         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_110049, whole genome shotgun sequence.
ACCESSION   DASWBY010000839 DASWBY010000000
VERSION     DASWBY010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110049"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /gene="mnmG"
                     /locus_tag="VF870_15150"
     CDS             <1..1366
                     /gene="mnmG"
                     /locus_tag="VF870_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX9253581.1"
                     /translation="FGEQPALGITESLAKLGFEYGRLKTGTPPRLKINSINWEILEEQ
                     PGDENPQPFSHLTDMSNFPFLPQVSCHITYTDPTVHKILEKGFAASPLFTGLIKGVGP
                     RYCPSIEDKIVRFSDKERHQLFLEPEGLDSDLIYLNGFSSSLDADIQLEALHKIVGLE
                     NVDMVRPGYAVEYDFFPPHQVDLTLETKLIKGLYFAGQINGTSGYEEAAAQGLVAGIN
                     AALKIQGKPEFVLKRSEAYIGVLIDDLVTKSTDEPYRMFTSRAEHRLLLRQDNADRRL
                     MKYGYEFGLIPKSLYDEVDNREKAIISGINYCHIKKYHARDINQFLINSGSNEIDSTE
                     TISKLCKRPEFNLKQLLEINGTANDLEAVALLQDEKALEQAEIEIKYEGYILRQQEAA
                     GKLEKYESTKIPLNYDFSKIKQLSTEGKEKLNKVKPRSIAQASRISGVTPSDISILLV
                     YLKN"
     gene            1383..>1605
                     /locus_tag="VF870_15155"
     CDS             1383..>1605
                     /locus_tag="VF870_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX9253582.1"
                     /translation="MPPTKQDNYLKELQSFCWDNGFNPEPMRNERLAFFAQLVAEKNK
                     TVNLISRKDIDSIIENHIFISAYVTKYLPE"
BASE COUNT          579 a          251 c          301 g          474 t
ORIGIN      
        1 atttggtgaa caaccggctc tggggattac tgaatcttta gctaagcttg gatttgaata
       61 cggaagatta aaaacaggaa ctccaccaag attaaaaata aattcaatca attgggaaat
      121 tttggaagag caaccagggg atgaaaatcc acaaccattt tctcatctaa cagatatgtc
      181 taattttcct tttcttcctc aagtaagttg tcatatcact tacactgatc caacagtgca
      241 taaaattctt gaaaaaggtt ttgcagcatc gccattgttt accggcttga taaagggagt
      301 tggaccaaga tattgcccat caattgaaga taagatagta agattttctg ataaagaaag
      361 acatcagctt tttttggaac ccgaagggtt agactctgat ttaatatatc taaacggttt
      421 ttcatcttct cttgatgctg atattcagct tgaagcgctg cataaaattg tcggtttaga
      481 aaatgttgat atggtacgtc ctggttatgc tgttgagtat gatttctttc cacctcatca
      541 agttgatctg actttggaaa ctaagttgat caaaggactt tactttgcgg ggcagataaa
      601 tggaacttct ggatatgagg aagctgctgc acagggatta gttgcaggaa taaatgcagc
      661 attaaaaatt cagggaaaac cagaatttgt cttaaagcga agtgaggctt acatcggagt
      721 tttaattgat gatcttgtaa caaaatctac agatgaacca tacagaatgt ttacatccag
      781 agcagagcat agactattgc ttagacaaga taatgctgat agaagattga tgaaatatgg
      841 ttatgagttt gggctaattc ctaaaagttt atatgatgaa gttgataatc gagaaaaagc
      901 aataatcagt ggtatcaatt attgccatat aaaaaaatat catgctagag atattaatca
      961 atttctaata aactcaggtt caaatgaaat tgattcgaca gaaacaatat caaaactttg
     1021 taaaaggccc gaatttaatc taaaacaatt actggagatt aacggaactg ctaatgatct
     1081 ggaagcagtt gccttgctgc aagacgaaaa agcactcgaa caggcagaaa tagaaataaa
     1141 gtacgaaggt tatattttgc gacagcaaga agctgctggc aagcttgaaa aatatgaaag
     1201 tacaaaaatt ccattgaact atgatttctc aaaaatcaaa cagttatcaa cagaggggaa
     1261 agaaaaactg aacaaagtta aaccgcgatc tattgctcag gcatccagaa tctcaggtgt
     1321 aacaccttca gatatttcca ttttgttggt atatttgaaa aactaatttg taggattttt
     1381 taatgccgcc taccaaacaa gataattatt taaaagaatt acaaagtttt tgttgggata
     1441 atggttttaa tcccgaacca atgcgcaatg aaagacttgc attttttgcg caacttgttg
     1501 cggaaaaaaa caaaacagta aatttaattt ccagaaaaga tattgatagc attatcgaaa
     1561 atcatatctt tatttctgcc tacgtaacaa aatatttacc agaaa
//
LOCUS       DASWBY010000840         5758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_72508, whole genome shotgun sequence.
ACCESSION   DASWBY010000840 DASWBY010000000
VERSION     DASWBY010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72508"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VF870_15160"
     CDS             complement(<1..332)
                     /locus_tag="VF870_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9253583.1"
                     /translation="MTFYKNHKEKLELLKSGKLSLVENVKHFLKNIEDKKDLNAFNFV
                     FNDEAIESARTVEQKIKNNSAGKLAGMAIAIKDVLAYKDHPLTCSSNILKNFTSLYTA
                     TAVQKLID"
     gene            complement(450..644)
                     /gene="tatA"
                     /locus_tag="VF870_15165"
     CDS             complement(450..644)
                     /gene="tatA"
                     /locus_tag="VF870_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9253584.1"
                     /translation="MFGNLGAGEIILIVLVILLLFGAKKIPELAQGLGKGMKEFKKSL
                     KDVEDEIKKTDDEVKKTDKS"
     gene            complement(655..1356)
                     /gene="purQ"
                     /locus_tag="VF870_15170"
     CDS             complement(655..1356)
                     /gene="purQ"
                     /locus_tag="VF870_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX9253585.1"
                     /translation="MSLKFGVVVFPGSNCDHDAYYSLRKVLNYDVEFLWHKDTDLKQS
                     SVILLPGGFSYGDYLRTGAIARFSPIMNSVISFANKGGIVIGICNGFQILLEAGLLPG
                     VMIRNNSLKFACKDVYLKTENRETIFSAEISEATKALKIPIAHGEGNYFADIELLNEL
                     EANRQILFRYSDEDGNINDENNPNGSRLNIAGIVNKNGNVMGMMPHPERACDPTLGRT
                     DGQLIFKSIANSILN"
     gene            complement(1353..1604)
                     /gene="purS"
                     /locus_tag="VF870_15175"
     CDS             complement(1353..1604)
                     /gene="purS"
                     /locus_tag="VF870_15175"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX9253586.1"
                     /translation="MFKATVLIKRRPSILDPQGVAVEKGAKHLGISNIKGTRIGKLIE
                     FDVDVIDRAAAEKEVNEYCSKLLSNPIMEDFEFKLEEVK"
     gene            complement(1608..2327)
                     /gene="pssA"
                     /locus_tag="VF870_15180"
     CDS             complement(1608..2327)
                     /gene="pssA"
                     /locus_tag="VF870_15180"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX9253587.1"
                     /translation="MNRFRITPSVIPNLFTAMNMFSGFFSIISASQGNFIYAGWLIII
                     AAIFDTLDGFMARLTKSSSELGVELDSLSDVVSFGVAPSFLLYTTYFHQFETFGVIIS
                     SLPMIAGGFRLARFNVQLVGFDKSYFTGLPIPSSALTIVCFILSFYNNGYPEKLQQFI
                     IPMVLLLSFLMVSKVKYDTLPKFSISAIKKKPLIYIAYLIALVVLIIYKIKGLFFIFV
                     FMIAFGIFRQIFKYFSNKKPE"
     gene            complement(2331..2975)
                     /locus_tag="VF870_15185"
     CDS             complement(2331..2975)
                     /locus_tag="VF870_15185"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEX9253588.1"
                     /translation="MFTKYGYSTMGVVFVVAFILIAISIYLDNNFIRIPLLLLSAFLI
                     IFTLNFFRDPERTVPNKENIVVSPADGRVLFVKEVLDDKFLNGKAKLVSIFMSPLNVH
                     VNRIPISGKVEYVKYIQGEYLAAFEDKASERNERNEIGITSQAGKVFFTQIAGFVARR
                     IISDLKVGDSVNIGNRFGMIKFGSRVDIIVPEQWQVKVKKDDNVTAGETILFEY"
     gene            complement(2975..3655)
                     /locus_tag="VF870_15190"
     CDS             complement(2975..3655)
                     /locus_tag="VF870_15190"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9253589.1"
                     /translation="MIETLKRNDIPFDEGNYQMIEYLDFYSVNDTKKVKVKDIGKKVA
                     LTNAFFFDYLKEYHIPTTFLRKDGDSSLKFSVSKELPFRIKILNSADKRNSKIFGIKE
                     GAELNLPVFEFHYGCGKESVISESHLISFDLCNPEDMKLITRICSKINAVLKSFFERR
                     NEILAEICCHFGKQEDKVFLVGDFSPASIKIFPKDESVKWTNPYKLSSAAEVRKYTDH
                     LYNIASVK"
     gene            3789..4115
                     /locus_tag="VF870_15195"
     CDS             3789..4115
                     /locus_tag="VF870_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX9253590.1"
                     /translation="MKGGMQGMLKQVQKMQAEMQRVQGELANMTVSEEAGGGMIKAVA
                     NGMKELVSIEIDPQVINSEEKEILEDLVVAAVNKALQSANKMAEEEMGKVTKGMIPPG
                     LNIPGF"
     gene            4118..4714
                     /gene="recR"
                     /locus_tag="VF870_15200"
     CDS             4118..4714
                     /gene="recR"
                     /locus_tag="VF870_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX9253591.1"
                     /translation="MQIAQPLLIAIEELSKLPGIGKKTAQRLALHILKNEQDSVDKLV
                     KAIADLKIKLHFCKRCFNITEDDLCEICKSVKRDTTKICVVEEASDVIAIEKTNEYNG
                     LYHVLGGVLSPLSGITAHDLKIKELLTRFENENISEIILAINPDTEGETTSLYLARLI
                     KPLNVKVTRIARGIPIGGDLEFADEATIGRAMLNRIDL"
     gene            4711..5448
                     /locus_tag="VF870_15205"
     CDS             4711..5448
                     /locus_tag="VF870_15205"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9253592.1"
                     /translation="MKSEWFKDWFNTQEYLNVYQHRNESDAETHIKLILENVNIPLNA
                     KILDMACGSGRHAIILARKSFDVTAVDLSENLLSIAKQSALDENLKINFVQSDIRNFK
                     TTEKFNLVVNLFTSFGYFETDEENFSVLQKAYDLLVDGGYFILDFFNSQFLMQNLVES
                     SEEIIGGVKIHQFRKIKENRVTKKIVITKNGNLSQFEESVRMFTKDELTMAIQNIGFD
                     IYKTFGDFLGNDFEQFSSPRLILICKK"
     gene            5445..>5758
                     /locus_tag="VF870_15210"
     CDS             5445..>5758
                     /locus_tag="VF870_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253593.1"
                     /translation="MNKIIFITLVLISFTACDLFNTRNAEAPDQTRSNWQPPVEPAIV
                     IENLKNSLSDKNVQNYIACFVDTIFAHQNYNFSASSEATSRYDILQNWGLNEERRYFT
                     SIT"
BASE COUNT         1955 a          972 c          942 g         1889 t
ORIGIN      
        1 tcatcaatca gtttttgtac tgctgttgcc gtataaagcg aagtaaaatt ttttaatatg
       61 tttgatgagc aggtgagggg atgatctttg taagcaagaa catctttgat tgcaattgcc
      121 attcctgcaa gtttgccggc agaattattt tttattttct gttcaaccgt tctcgcagat
      181 tcaatagctt catcattaaa cacaaagttg aatgcgttaa ggtctttttt atcttcaata
      241 ttctttaaaa aatgctttac attttcaaca agactaagtt tacctgattt aagaagttct
      301 aatttttctt tgtggttttt atagaaagtc atcctattaa ttatgttttt tcattttaaa
      361 aaatagattc ccgcctaaag cgggaatgac aaaataattt ttagatttct ctcccgataa
      421 atcgggatcg aaatgacaca gcaagtgaac taagacttat cagttttctt tacttcgtca
      481 tcagtttttt tgatttcgtc ctcaacatct tttaaggatt ttttgaattc cttcatccct
      541 ttgcccaaac cctgagcaag ttcaggaatt ttctttgcac caaataatag aagtataaca
      601 agtacaatca gtattatttc gcctgcgcct aaatttccaa acataacttc acctttagtt
      661 taatatagaa tttgctatcg atttaaaaat taactgcccg tctgtcctgc caagagttgg
      721 atcgcaagct ctttcaggat gcggcatcat tcccattaca ttaccgtttt tgtttacgat
      781 tcctgcaatg ttaagtcgcg aaccatttgg attattttca tcattaatat ttccatcttc
      841 atctgaatat ctaaaaagaa tttgcctgtt tgcttcaagt tcgtttaaca attcaatatc
      901 tgcaaaataa tttccttcac catgtgcgat aggaatcttt agtgcttttg ttgcttcact
      961 aatctcagca gaaaaaattg tttctctatt ttcagtttta agataaacat ctttacaagc
     1021 aaacttaagg gaattattgc gtatcataac tcctggcagc aaaccagctt ccagcaaaat
     1081 ctgaaaacca ttgcagatgc cgatcacaat tccgcctttg tttgcaaagc ttattacaga
     1141 attcataatc ggagaaaatc ttgcaattgc tccggtgcga agataatcac cgtaagaaaa
     1201 tccacccggt aaaagtatta cgctgctttg tttcaaatct gtgtctttat gccagaggaa
     1261 ttcaacatcg tagttaagaa ctttgcgtaa agaataatat gcatcgtgat cacaattgga
     1321 accagggaaa actaccactc caaactttaa actcatttta cttcttcaag tttaaattcg
     1381 aaatcttcca taataggatt tgagagaagc tttgaacagt actcatttac ttctttttca
     1441 gcagcagcac gatcaataac atcaacatca aattcaatta gttttccgat tcttgttcct
     1501 ttaatatttg agatgccaag atgttttgcg cctttttcaa ctgcaacacc ttgcggatca
     1561 agtatggaag gtcttctttt aattagaact gtagctttaa acacttttta ctcgggtttt
     1621 ttatttgaga aatacttaaa aatctggcga aaaataccaa atgcaatcat gaaaacaaaa
     1681 atgaaaaata atcctttaat cttataaatt atcaaaacta caagggctat cagatatgca
     1741 atataaatca aaggtttttt cttgatggcg ctgatcgaaa actttggaag tgtatcgtac
     1801 tttactttgc ttaccattaa aaatgataat agtaaaacca ttggaattat aaattgttga
     1861 agtttttccg gatacccatt attataaaaa gacaagataa agcaaacgat tgttaacgct
     1921 gaagagggaa ttggtaaccc ggtaaaatat gatttatcaa atccgacaag ctgaacgtta
     1981 aatctagcca atctaaatcc gccggcaatc attggaagtg aactgattat cactccaaaa
     2041 gtttcaaact gatggaagta agttgtataa agcaagaaag aaggcgcaac accaaaactt
     2101 acaacatcag aaagtgaatc tagttcaaca ccgagttcac tgctggattt tgtgaggcgg
     2161 gccataaaac catcaagtgt atcaaagata gcggcgataa ttatcaacca gcctgcgtaa
     2221 ataaagtttc cctggcttgc gctgattatc gaaaaaaaac cgctgaacat gttcatagcg
     2281 gtaaataaat tcggtattac agaaggtgta attctaaatc gattcattca ttaatattca
     2341 aaaagaattg tttcgcctgc agtaacgttg tcgtcttttt ttacttttac ctgccactgc
     2401 tcgggaacaa ttatatcaac acgactccca aatttaatca taccaaatcg atttccaatg
     2461 tttaccgaat ctccgacctt aagatcactt ataattcttc ttgcaacaaa cccggcaatt
     2521 tgagtaaaga atacttttcc tgcctgactt gtgattccaa tttcatttct ctcgtttcgt
     2581 tcagatgctt tatcttcgaa tgctgccaga tattcacctt gaatatattt tacatattct
     2641 actttgccgg aaattggaat tctgtttacg tgaacattta acggagacat aaatattgaa
     2701 actaattttg ctttgccatt taagaattta tcatctaaaa cttctttaac aaataaaact
     2761 cttccatcag caggtgatac aacaatgttt tctttatttg gaactgttcg ttcagggtca
     2821 cgaaaaaagt taagagtaaa aatgatgaga aaagcagaga gtaataaaag cggtattcta
     2881 ataaaattgt tatctaaata aatgctgatc gcgatcaaaa taaacgcaac aacaaatact
     2941 actcccatag tactatagcc gtatttggta aacattattt aacgctcgct atattataaa
     3001 gatgatcggt atattttctt acttcagccg cagaagaaag cttatatgga ttagtccatt
     3061 ttactgattc atcttttgga aaaatcttta tacttgccgg agaaaaatca ccaaccagaa
     3121 aaactttatc ttcctgcttt ccaaaatgac agcagatttc ggcaagaatt tcatttcttc
     3181 tttcaaaaaa tgattttaaa accgcattaa tctttgaaca gattctggtg atcaatttca
     3241 tatcttcagg attgcacaga tcaaaagaaa ttaaatgact ttcgctgatt acactttctt
     3301 ttccgcagcc ataatgaaat tcaaaaacag gcaggttcaa ttcagcacct tcctttatcc
     3361 caaatatttt ggaatttctt ttatcagcag agttaaggat tttaattcga aatggaagtt
     3421 cttttgaaac agaaaatttt aaagatgaat caccatcttt tcttaaaaat gttgttggta
     3481 tgtgatattc tttcagataa tcaaagaaaa aagcattagt taatgctact tttttcccaa
     3541 tatcttttac cttaaccttt ttagtatcat taacagaata aaaatcaaga tattcgatca
     3601 tctgataatt accttcgtcg aaaggaatat catttcgttt taatgtttca atcatttttc
     3661 ctcgattatc aaaatttact aactaaaatt cagggcagaa attaggcata ttaaatgtat
     3721 aaagtcaatc gtttatatta tatttggctt ccaaaaaaca caaaaaatta taaaaacagg
     3781 agatatttat gaagggcgga atgcaaggaa tgcttaaaca ggtgcaaaaa atgcaggctg
     3841 agatgcaacg cgtgcaaggg gaacttgcca atatgacagt ttctgaagaa gctggcggcg
     3901 gaatgattaa agccgtggca aatggtatga aagagttagt ttctatcgaa attgatcctc
     3961 aggttataaa ttctgaagaa aaagagattt tagaagattt agttgttgcg gctgtaaata
     4021 aagcattaca atcagctaac aaaatggctg aggaagagat gggaaaagtc actaaaggta
     4081 tgatccctcc aggattaaac attccgggat tttagctttg caaatagcac aaccgctttt
     4141 aatcgcaatt gaagagttaa gcaaacttcc gggaatcggg aaaaagactg ctcaaaggtt
     4201 agcattgcac attttaaaga atgaacagga ttctgtggat aagcttgtga aagctattgc
     4261 agatttaaag ataaaattac atttctgtaa acgatgtttt aatataaccg aagatgatct
     4321 ttgcgaaatt tgtaaaagcg ttaagcgcga tacaacaaag atttgtgtcg ttgaagaagc
     4381 aagtgatgtt atcgcgattg aaaaaacaaa tgagtataat ggattgtatc acgttttagg
     4441 cggtgtgctt tcgcctcttt ccggtattac cgcacacgat ttaaaaataa aagagcttct
     4501 tacaagattt gaaaatgaaa atatttccga aataatactt gcaataaatc ccgatactga
     4561 aggagaaaca acttcacttt atttagcaag attaataaaa cccctaaatg ttaaggttac
     4621 aagaattgcc agaggtattc ccattggcgg tgatcttgaa tttgccgatg aagccacgat
     4681 tggcagggca atgctgaatc gaattgattt atgaaatccg aatggtttaa agattggttt
     4741 aatactcagg aatatttaaa tgtttatcag catcgaaatg aaagtgatgc agaaacacat
     4801 ataaaactca ttttggaaaa tgttaatatt cctttaaacg caaaaattct tgatatggcg
     4861 tgcggatccg ggagacatgc aattattctt gcaaggaaaa gctttgatgt tactgcagtt
     4921 gatttaagtg agaacttatt atctatcgcc aaacaatccg cattggatga aaatcttaaa
     4981 ataaattttg ttcagtctga tataagaaat tttaaaacca ctgagaagtt taatctggtt
     5041 gtaaatcttt ttacaagctt tggatatttt gaaactgatg aagaaaattt ttcagtactt
     5101 caaaaagctt atgatctttt agttgacggc ggttatttta ttcttgattt ttttaactcg
     5161 caatttttaa tgcaaaatct tgttgaatcc tctgaagaga ttattggtgg agttaaaatt
     5221 catcagttca gaaagatcaa agaaaacaga gtaacaaaaa aaatagttat tactaaaaat
     5281 ggcaatctta gtcagtttga agaatcggtt agaatgttta ctaaagatga attaactatg
     5341 gctatccaaa atattggatt tgatatttat aaaacatttg gtgattttct tggtaatgat
     5401 tttgagcaat ttagttcacc acgattgatt ttgatttgta aaaaatgaat aaaataattt
     5461 ttattacttt agttctcatt tctttcacag cttgtgatct ttttaataca cgtaatgcag
     5521 aagcaccaga tcaaacaaga tcaaactggc agcctccggt tgaacctgct attgttattg
     5581 agaacttaaa aaactctcta tcagataaaa atgttcagaa ctacatcgcc tgttttgtgg
     5641 atacaatttt tgcacatcaa aattataatt tttcagcatc aagtgaagct acctcacgtt
     5701 acgatatcct tcaaaactgg ggtttgaatg aagaaagaag atattttaca agtattac
//
LOCUS       DASWBY010000841         2798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_628964, whole genome shotgun sequence.
ACCESSION   DASWBY010000841 DASWBY010000000
VERSION     DASWBY010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628964"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VF870_15215"
     CDS             <1..641
                     /locus_tag="VF870_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX9253594.1"
                     /translation="SPFLSGIVSFIFLGGVVAGLAYGIGAKTIKNDNDVIKGMNKSME
                     TLGSYIVLVFFAAQFVAFFNWTNLGMIVAVEGANLLKSSGLGPIPLLISFIIISGFIN
                     LFIGSASAKWAIMAPVFIPMFMLLGYSPELVQGAYRVGDSVTNIISPMMSYFALIIAF
                     VGKYDSKTGIGTLIATMLPYTITFFIVWTIMFVVWFLLGIPVGPGAQLYINN"
     gene            complement(699..1217)
                     /locus_tag="VF870_15220"
     CDS             complement(699..1217)
                     /locus_tag="VF870_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX9253595.1"
                     /translation="MKYLMIITILSSIMFSQQNPPTTVKYVDLNKYVGLWYEISKIPN
                     SFQDQCIKGTTAKYIFSEDGEIRVINSCTDKNGEVDEADGLARVVDKKTNSKLEVSFF
                     SIFGWRPIWGDYWIIGLDENYQWAIVGTPNRKYGWVLGRTPKLESETMDKINQILKDQ
                     GYDPKKFEISKQ"
     gene            complement(1222..2358)
                     /locus_tag="VF870_15225"
     CDS             complement(1222..2358)
                     /locus_tag="VF870_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEX9253596.1"
                     /translation="MKPSERLLSLDVFRGITIMGMILVNNPGTWSAIYPPLEHAKWNG
                     CTPTDLIFPFFLFIVGVAISYSLSKRKAQGGDMKSLYLNIFRRTAILFGLGLILSGFP
                     FGLLFGHQFSLSTLRIPGVLQRIAVVYMISSVLFLSTNTKFQYWFTGSILVIYAMLMS
                     FIPVPGIGHANFEPTTNLSAWLDRLILGNHIWSGSKFWDPEGILSTLPAIGSAMLGIF
                     TGNWLRSEKDQTTKTVWLFVWGSILMVAGWVWDGWFPINKNLWTSSYVLYTAGLALNF
                     LAFCYWFIDVKKITWWIKPFQVYGMNAITVFFLSGIVGRIMYMVKWETADGTVVTIKD
                     YLFNTFFLSWLQPINASLAWAIVYILFWLGLMWILYAKKIFIKV"
     gene            2554..>2798
                     /locus_tag="VF870_15230"
     CDS             2554..>2798
                     /locus_tag="VF870_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253597.1"
                     /translation="MKKLILIVMIAISINAQTKFTDVNKFVSSGEFKKATEMIDKKLE
                     SNNLSYDEIFELQFEKERLDRIRKDFNKTAEDVLKVV"
BASE COUNT          911 a          511 c          459 g          917 t
ORIGIN      
        1 aatccccatt cttaagcgga atcgtttcat tcatatttct tggtggtgtt gttgccggtc
       61 tggcttatgg tatcggggca aagactatta aaaatgataa tgatgttatt aaaggtatga
      121 ataaatcgat ggaaactttg ggttcttaca tcgttcttgt tttttttgca gctcagtttg
      181 ttgcattttt taactggaca aaccttggaa tgattgtcgc ggttgaaggt gcaaatcttt
      241 taaaatcttc aggactcgga ccgataccac ttttaatttc attcatcatt atatccggat
      301 ttattaattt atttatcggt agtgcttctg ccaaatgggc aataatggct cctgttttta
      361 taccaatgtt tatgctttta ggatattcgc ctgagttagt tcaaggtgca tatagagtcg
      421 gtgatagtgt gacaaatata atctcaccca tgatgtctta ttttgctttg ataattgctt
      481 tcgttggtaa gtatgattct aaaaccggaa ttggaactct tattgccaca atgctaccct
      541 acacaataac attttttatt gtctggacta taatgtttgt ggtgtggttc ttattaggaa
      601 tacctgtagg accaggtgct caattgtaca taaataacta gaataaaata ttataatttc
      661 tcatgcccct actcaggagg ggctgagaaa gattcatttt actgcttact aatctcaaac
      721 ttcttgggat cgtaaccctg atcttttaaa atctggttga tcttgtccat tgtttcactc
      781 tccaattttg gagtacgacc taaaacccag ccatattttc tatttggtgt tccaacaatt
      841 gcccattggt aattctcatc aagaccaatt atccagtaat ctccccaaat aggcctccag
      901 ccgaaaatgc tgaagaaact tacttcaagt tttgaattag tttttttatc aacgactctt
      961 gcaagtccat ctgcttcatc tacttcgcca tttttatcag tgcaggaatt tattactcta
     1021 atctcaccat cttcgctaaa aatatatttt gctgtagtac ctttaataca ttgatcctga
     1081 aagctgtttg gtatttttga gatttcatac cacaaaccga catatttatt aagatcaaca
     1141 tatttaactg ttgttggcgg attttgttgt gagaacataa tgcttgataa tattgttata
     1201 atcattagat acttcattct gttaaacctt tataaagatt ttctttgcat agagtatcca
     1261 catcaaccca agccagaata aaatgtacac tatagcccag gctaatgatg cgttaattgg
     1321 ctgcagccac gataagaaaa atgtattaaa taaataatct ttaattgtaa ctactgttcc
     1381 atctgcagtt tcccacttaa ccatatacat aattcttcct actattccgg atagaaagaa
     1441 aactgtaatt gcattcattc catatacctg aaaaggtttt atccaccagg taattttttt
     1501 aacatcaatg aaccaataac aaaaagccag aaaatttaga gctaatccag cagtataaag
     1561 tacgtaagaa cttgtccaaa gatttttatt gattggaaac catccatccc aaacccagcc
     1621 ggcaaccatc aatattgatc cccaaacaaa aagccaaacg gttttagttg tttgatcctt
     1681 ttcacttctt agccaatttc ctgtaaatat accaagcatt gcactgccaa ttgcaggaag
     1741 agtagaaagt attccctcag gatcccaaaa tttactacct gaccaaatat gatttccaag
     1801 tattaatcga tctaaccatg cagaaaggtt tgttgtcggt tcaaaattag catgcccgat
     1861 acccggaact ggaataaaac tcattaacat agcatatata actagtattg agcctgtaaa
     1921 ccagtactga aactttgtat tcgttgaaag gaaaagtact gaagaaatca tgtacacgac
     1981 ggcaattctt tgcagcactc ccggaatgcg aagtgtgctt aaagaaaatt ggtgcccgaa
     2041 taataaccca aacggaaatc cggaaagtat taatcctaat ccaaaaagta ttgccgttct
     2101 tcgaaaaata ttcaggtata aacttttcat atcgccgcct tgagcttttc ttttactcag
     2161 cgaatatgat attgctactc caacaatgaa aagaaagaat ggaaaaatta aatcagtcgg
     2221 tgtgcaccca ttccatttag catgttctaa aggcggataa attgcactcc aggtcccggg
     2281 attgtttaca aggatcatcc ccattattgt tattccccta aaaacatcaa gcgaaagcaa
     2341 acgttcggaa ggtttcataa tatttccata agtagttgaa aattattgta acaaaaataa
     2401 gtaaagtttg atgaataatg tttaaagctt tgttattttt agatagttat tctaaacctg
     2461 tctgccaaaa agcaatcagc ttaggaatct caattataat tgtcaggatc ctgaaattaa
     2521 ttcaggatga ctatttatca gcctggaaaa tttatgaaaa aattaattct gattgtgatg
     2581 atagcaattt ccataaacgc acaaacaaaa tttactgatg taaacaaatt tgtcagctca
     2641 ggtgaattta agaaagcaac tgaaatgatt gataagaaac ttgaatccaa taatctgtct
     2701 tatgatgaaa tatttgagct tcaattcgag aaagaaagat tagatcgtat cagaaaagat
     2761 tttaataaaa ctgcagaaga cgttctgaag gttgtaaa
//
LOCUS       DASWBY010000842         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_261567, whole genome shotgun sequence.
ACCESSION   DASWBY010000842 DASWBY010000000
VERSION     DASWBY010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261567"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1299
                     /locus_tag="VF870_15235"
     CDS             154..1299
                     /locus_tag="VF870_15235"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX9253598.1"
                     /translation="MIISVPKEIMPGENRVACVPDVVSKLIKAGYEVQIEKDAGLSAG
                     FTNEQYQKAGAKIIDNLSDLYANADLVFKVQRPIDHPTAGKNEIDLMKKGTILVSFVY
                     VLHYSDIAKKCAEKGIDLISMDMIPRTTLAQKMDVLSSQANIAGYKSVLLSANELGKI
                     FPLMMTAAGTISPAKVVIMGAGVAGLQALGTAKRLGAVVEVSDIRTAVKEEVQSLGGR
                     FIEVEGAEDMQDAGGYAKEASQEFLQKQKETIFKHVTEADIVITTALVPGKKAPILVS
                     EEMVKHMRPGSVVLDMATEFGGNCEVSEKGKTVKKYGVTIIGESNLPSLVPTHASEMY
                     SKNILSLILHIGKEGKVALNLNDEIVKGSLITQNGEVINQRVKDLIK"
     gene            1324..1608
                     /locus_tag="VF870_15240"
     CDS             1324..1608
                     /locus_tag="VF870_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX9253599.1"
                     /translation="MEDYLFLMHVYVFALAIFVGFELITKVPPTLHTPLMSGSNAISG
                     ITIVGAILSAGLEQFTISTILGLIAVAFAMINVVGGFLVTDRMLKMFKKK"
     gene            1617..>2100
                     /locus_tag="VF870_15245"
     CDS             1617..>2100
                     /locus_tag="VF870_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX9253600.1"
                     /translation="MRIAIEITYLISSILFIFGIKFLGSPKTARKGNLYAAIGMFLAI
                     AATLLDQQVLTYEWIIIGALIGSVAGLIMAYKTPMTGMPQMVGMLNGFGGGASTLVAL
                     SEYFKKNPNYPVDTGLPFNLPVDQGIAIILSLLIGGVTFTGSMIAFGKLQGLITGKVV
                     K"
BASE COUNT          693 a          331 c          429 g          647 t
ORIGIN      
        1 caaacaatat acttgtctta aatgttgttg taattaaagc tgctgtaaaa aaatctgtta
       61 caataaatta agagtatttt tattggatta gctttcatta gtgcttagtt ttgtaactaa
      121 aattttaatt ctatcaacag aggagataat tctgtgataa tttcagttcc aaaagagata
      181 atgcccggtg aaaatcgtgt tgcctgtgtt ccggatgttg tttctaaact aattaaagcc
      241 ggatacgaag ttcagattga aaaagatgca ggacttagtg ctggttttac taatgaacaa
      301 tatcaaaaag caggcgcaaa aattattgat aatctttccg atctttacgc caatgctgat
      361 ttagttttta aggttcagcg acccatcgat catccaacag caggtaaaaa tgaaattgat
      421 ttaatgaaga agggaactat tcttgtttct tttgtttatg ttcttcatta ttcagatatc
      481 gcaaaaaaat gtgcggaaaa agggatcgat ttgatttcaa tggatatgat tccaagaaca
      541 acgcttgcac agaaaatgga tgtgcttagc tctcaggcaa acattgccgg ctataaaagt
      601 gttttacttt ctgccaatga acttggtaaa atttttccgc ttatgatgac tgcagccgga
      661 acaatttctc ccgctaaagt agtaataatg ggtgcaggtg ttgcaggatt acaagcactt
      721 ggtaccgcta aaagattagg cgccgtggtt gaagtttcag acattcgtac cgcagtaaaa
      781 gaagaagtac aaagtcttgg cggtagattt attgaagtag aaggtgcaga agatatgcag
      841 gatgccggag gctatgccaa agaagcatct caagaatttt tacagaagca aaaagaaaca
      901 atatttaagc atgttacgga agctgatata gttataacta ctgcactggt tcccggtaag
      961 aaagctccaa tactcgtttc cgaagaaatg gtaaaacata tgcgaccggg cagtgttgtt
     1021 cttgatatgg ctactgagtt cggtggtaac tgcgaagtaa gcgaaaaagg taaaaccgtt
     1081 aaaaaatacg gcgtaactat tattggcgaa tcaaatcttc cttctttagt cccaacgcat
     1141 gcaagcgaaa tgtattcaaa aaacatactt agtttaattc ttcatatagg taaagaagga
     1201 aaggttgctt taaaccttaa tgatgaaata gtaaaaggat ctttaataac tcagaatggt
     1261 gaagttataa accaaagagt taaagatcta attaaataaa gtttaaaaat ataaaggtag
     1321 attatggaag attatttgtt tttaatgcac gtgtatgtat ttgcacttgc gatatttgtg
     1381 ggttttgaac ttataaccaa agttccccca acactgcata cgcctcttat gtctggctca
     1441 aatgcaatat ccggaattac aattgttgga gcaattttaa gtgccgggct tgagcagttt
     1501 actatcagca caatcttagg attaattgcg gtagcattcg caatgataaa tgttgtcgga
     1561 ggatttcttg tcaccgaccg tatgttaaaa atgtttaaaa agaagtgagt tgaaaaatga
     1621 gaatagcaat agaaataaca tatctgattt cttcaattct cttcatcttt gggatcaaat
     1681 ttcttggatc accaaagacc gcaagaaaag gaaatcttta cgctgcaata ggaatgttcc
     1741 ttgcaattgc agcaacttta ctcgatcagc aagttttaac ttatgaatgg atcataattg
     1801 gggcattaat aggatcagtt gccggattga ttatggctta caaaactcct atgacaggaa
     1861 tgcctcaaat ggttggaatg cttaatggat ttggtggcgg tgcgtcaacg ctagttgcac
     1921 tttcggagta ctttaaaaaa aatcctaatt accctgtaga tactggatta ccgtttaatc
     1981 taccggttga ccaaggaatt gctattatac ttagtttact tattggtggt gttactttca
     2041 caggatctat gatagctttt gggaaacttc agggcctaat aaccggaaaa gtggttaaat
//
LOCUS       DASWBY010000843         7231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_750133, whole genome shotgun sequence.
ACCESSION   DASWBY010000843 DASWBY010000000
VERSION     DASWBY010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750133"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2415
                     /locus_tag="VF870_15250"
     CDS             <1..2415
                     /locus_tag="VF870_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9253601.1"
                     /translation="KFRFLGYKTRTVENIRISADQTTKLDAGLELEAIVGEEVIVVAK
                     KPLVEYNQTSSVSTVNKDDIKNLPIQSLSEIVNLQAGVIDGHFRGGRIGEVQYQVDGV
                     TVNNPFSNSSMLEIDRSVIEEVQVISGTFDAKYGQAMSGVVNTVLKTGTDKFEYSLET
                     YLGDYFTTDTDRYPHNDSYNPTTIQNYQLSFSGPTGLPQTTFFINGRKYLNDGYMFGT
                     RTFIPFDKNDFQNQIYNPTGDGELVSMGTSDEWNGQFKITNQTIKDLQFNYQLSYNYA
                     ERTGYNHAFRLNPDGIKTNYTNSLSHGLTVTQTLSPEMFYKINIRQNLFEYNDYKYED
                     LYDPRYLEAGEPKSDANYEDGAIVQGVDLGRYQQKTNSLIVKADYTWQANRFNMIEAG
                     LEGQYSDVLFGPPGFFISSFNDSTGAQVLVPVESAYHQPGLQSYYPKQFAAYLQDRVE
                     LGDLVIRAGLRFEYYDAAGQVPSDLQNPANSIDGAPTSTYVPTTPKTEIAPRLGLSFP
                     LTSAASVYFSYGHFYQLPALDYLYSNADYSILEDLQAGGVSYGVMGNPDLSPEFTIQY
                     EFGLKQTVSDFLGVQLSFFYKDIRDLLGVEFISTYTAAEYARFANTDFGSVYGFTLSL
                     FQRNLGYFNTSIDYTLQFAQGNSSDPRETANRAEAGQDPRPRNLPFNWDQTHTLNLSA
                     IYAAPNDYSLSAIFRFGSGQPYTPEMGTGFGATLETNSGRKASYVLLDLRAEKYFDFD
                     FINLSVFLRMFNVLNSTFVNGFVFNSTGSPDYTLNPASNPAALYDPSRFYQPRRIEFG
                     ISLRSN"
     gene            2418..5714
                     /locus_tag="VF870_15255"
     CDS             2418..5714
                     /locus_tag="VF870_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253602.1"
                     /translation="MKKINTLNIPKASKYSVGIMHLTYLVFLLIMILVSQAKAQILPD
                     PVPPEWRGAIDAEREGMHDANQIRTVFLNYGMVGDYPPDPGNVDLSIFHSVEIPKGSG
                     ENYSDGTTPFVLANVTMLNGNPAYIMETGYRERQGTSPITNKTMRFEPRFGYFQIDPN
                     INKGRSSAISNDPRTWPDEWYDKRNDPDDPGWSGSWNGFFGKAPKADQESYVVYDDNF
                     YDAWNYYPDSRDNTRRGLGMRVEQRGFQWANPQAGLVIFFHYDITNESTTSYNNNVIF
                     GLYMDSGVGGSALGPNGVYESDDDNAFFDKSAGLNLVYTWDNYGNGHIGPTGYLGYSY
                     LETPGNAVDGIDNDEDGILDERRDSGPGQQIIGQDAILNYVTANYDLAKFEAYNGAIT
                     DRPAFTEGVWWTGDEDLDWVAEYYDTGADGIFGTNDTGEKDGQPTNGETNFDKTDIDE
                     SDQIGLTGFKLNRIKAGAGNPSTVVDQIVFFDDGKQWPKKLYNIFTNPDSAFGAPEAL
                     NYNIGFLFASGPFYLGAGNRERFSLALGFGANLRELRVTTKVVQQIYKANYQFAIPPP
                     MPTVNAFTGDGFVTLTWDNAGERAFDPITNNNDFEGYRVYRSTDPAFLDPQVIYTATG
                     TRPFASKPIAQYDLINGISGFSTTTVEGIAYFLGEDTGIKHTFTDTTVVNGQRYYYAV
                     VSYDRGVDSSQIYPSENAITISQTLRGGTILPQNAVEVYPNPPSVGFQDAQTLNLVQS
                     EGRGSGEVSLQVLNSAIVPENHTFAIKFDNASDSVRAESYYLVDETLSDTLFTLGVDF
                     DGLGAGPSGGGILPIIYTPLVTEIDSQHTGLVSGSGTNIPLKIRYSDVLPINLKRPGF
                     PENIDVIFSDIVIDTSLADPVNFIPARPVKFTVIAKTEAGDQKLKFTFYGGSNSTNGI
                     LDAHGDYISILTAATETPTQRKPTWRLEVDSTGLNGAPIVPPTINDKYELRLIFPYSS
                     QDKFTFLTKAQYIDPNLAKQQFEKDPYVVPNPYVGAASFEPQRFAVSGRGERKIEFRN
                     LPANCTIRIYTVTGEFVKELAHDGNINEGIVSWDLRNSSNLEIAPGLYIYYVDGPGVG
                     TFIGKFAIIK"
     gene            5728..6765
                     /locus_tag="VF870_15260"
     CDS             5728..6765
                     /locus_tag="VF870_15260"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9253603.1"
                     /translation="MKKLIRINIKVVSTCFLIVFLFAGQNFGQNKTGTTIGQFLKIEP
                     SSRVVALGNAGASLSGEISNIFYNPASLGRLQGADIQFTFNKWLADITYNYMAAGINV
                     EGVGTFALVGTLLNSGEMDVRTVESPLGTGERFTVNNLALGLGYGLMLTDRVSVGIQI
                     NYINETIWHSSLSTFGLNFGVQYQVVEGGLTLGASVSNFGTSAAYDGRDLYLDYDFDP
                     DKYGDNDKLPAELRTDNFNLPTLFRAGVSYWFDFGENYKLLVAADAVHPNDNDQYLNL
                     GAEFNLLKIFSIRAGYRNWLMPEAEGGLVLGGGVQTNFNETNNIRFDYAYADYGRLAE
                     THRITLSVGFR"
     gene            6783..>7231
                     /locus_tag="VF870_15265"
     CDS             6783..>7231
                     /locus_tag="VF870_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253604.1"
                     /translation="MKQYFEMKTINTILLVVFLFVGCKAQQDQIVFKSNGKVDYPLSN
                     SDEKLLDSIQYRSFLFFKEQSNPENGLVKDRSASWAPASIAAIGFALPSYAVGVERNW
                     MSRDEAAMITLNTLNFFLNSVQSSDTNATGYRGFYYHFLRMNSGTRE"
BASE COUNT         2307 a         1224 c         1493 g         2207 t
ORIGIN      
        1 aagtttagat ttttaggcta taaaaccagg actgtcgaaa atatcaggat ctcagccgat
       61 caaacaacga agcttgatgc gggtttagaa ttagaagcta tagtgggtga agaagttata
      121 gttgttgcca aaaaaccact agttgaatat aatcaaacca gttctgtgtc aactgtaaat
      181 aaagatgata ttaaaaatct gccgatacaa agtttaagtg aaattgtaaa tctgcaggcc
      241 ggtgtaatag atggtcactt tagaggtggc agaattgggg aggttcaata tcaggttgat
      301 ggtgtaacag taaacaatcc attcagtaac agctcgatgc tggaaattga tcgttcggtt
      361 attgaagaag ttcaggttat cagcggtacg tttgatgcaa aatacggaca ggcaatgtcc
      421 ggcgttgtaa atacagtatt aaaaaccgga actgataagt ttgaatattc acttgaaaca
      481 tatttgggtg attattttac tacagataca gaccggtatc cacataacga cagctataat
      541 ccgactacta ttcagaacta tcaattatcg tttagcggcc caacagggct gcctcaaact
      601 acattcttta taaacggtag aaaatattta aatgatggat atatgttcgg aaccagaaca
      661 tttattccct ttgataaaaa tgattttcaa aaccagatct ataatccaac aggcgatggt
      721 gagcttgtaa gtatgggtac ttctgatgaa tggaatggac agtttaaaat cacaaatcag
      781 acaattaaag atctacaatt caactatcaa ctatcttata attatgcaga acgcactggg
      841 tacaatcatg cattcagatt aaatccggat ggaataaaaa ctaattatac aaattcgctt
      901 agccacggtt tgactgttac tcagacactt tctccggaaa tgttttataa aattaatatc
      961 agacaaaatt tatttgagta caacgattat aaatatgaag acttgtatga tccaagatat
     1021 ttagaagctg gcgagcccaa aagcgatgcg aattatgaag atggagcaat tgttcaaggt
     1081 gttgatctgg gaagatatca acaaaaaacg aactccttaa ttgtaaaagc agattataca
     1141 tggcaggcaa acagatttaa tatgattgag gcaggtttgg aaggacaata ttctgatgta
     1201 ttattcgggc caccgggatt ttttatctct tcattcaatg atagtactgg tgctcaagtt
     1261 cttgttccgg ttgagagcgc ttatcaccag cccggtctgc aatcatatta tcctaaacag
     1321 tttgcagctt atctgcaaga tagagttgaa cttggagatt tggttataag ggcaggttta
     1381 cgttttgaat attatgatgc tgccggacaa gttccgagtg atttacaaaa tcctgcaaat
     1441 tccattgatg gcgcaccgac ttcaacttac gtcccaacga ctcctaaaac agaaattgca
     1501 ccaagactcg gtttgagttt tcctttaact tcagcagcat cagtttattt ttcttacgga
     1561 catttttatc aattgcctgc cttggattat ttgtacagca acgctgatta ttctatactg
     1621 gaagatttac aggccggcgg agtaagttat ggtgttatgg gcaatccgga tttaagtccc
     1681 gaattcacaa ttcaatatga gtttggactt aagcaaactg tctctgattt tctaggagtt
     1741 caactttcat tcttttataa agatattcga gatcttctgg gtgtagagtt tataagtact
     1801 tacactgctg cagaatatgc aagatttgct aatactgatt ttggaagtgt ttacggattt
     1861 actttatcac ttttccaaag aaaccttggt tacttcaata cttcaattga ttatacactt
     1921 caatttgctc agggtaattc aagtgatccc agagaaactg caaatagagc agaagccggg
     1981 caagatcccc gtccgagaaa tttacccttt aactgggatc aaactcatac attaaatctt
     2041 tcggccattt atgctgctcc aaatgattat tcacttagtg caatttttag atttggaagc
     2101 gggcagcctt atactcctga aatgggtact ggttttgggg ctacgttgga aaccaactcc
     2161 ggaagaaagg ccagttacgt cttacttgac cttcgtgcag aaaaatattt tgattttgat
     2221 tttataaacc tgagtgtttt tctcagaatg tttaatgtac ttaactcaac ttttgtaaat
     2281 ggatttgttt tcaattcaac aggaagtcct gattataccc ttaacccggc ttccaatccg
     2341 gctgccttgt atgatccttc acgattttat cagccccgca gaattgaatt cggtatctct
     2401 ttgaggagca attaactatg aaaaaaataa acacattaaa tattccaaaa gcatcaaaat
     2461 attcagttgg aattatgcac ttaacttatt tagtgttttt actaataatg atattggttt
     2521 ctcaagccaa agctcaaatt ctacctgatc ctgttccacc tgaatggaga ggtgcaatag
     2581 atgctgaaag agaaggaatg catgacgcaa atcaaatcag aacagttttt ctaaattatg
     2641 gtatggtcgg tgattatcca cccgacccgg gaaatgtaga tctttcaata tttcattcgg
     2701 tggaaattcc taaaggctct ggtgagaatt acagcgatgg aaccacacca tttgttcttg
     2761 cgaatgttac tatgttaaat ggcaaccctg catatataat ggaaacaggt tatcgagaaa
     2821 gacagggaac aagtccaatc acaaataaga ccatgcgttt cgaacctcgt tttggatatt
     2881 ttcagattga tccaaatatt aataaaggaa gatcttctgc aatcagtaat gatccgagaa
     2941 cctggccgga tgaatggtac gacaaacgta atgaccctga cgatccgggg tggagcggct
     3001 catggaatgg gttttttggt aaagcaccaa aagctgatca ggaaagttat gttgtttatg
     3061 atgataactt ttatgatgca tggaattatt atccggatag cagggataat acaagacgag
     3121 gtctgggaat gcgcgtagag caaagaggat tccagtgggc aaatccacag gcaggattag
     3181 taattttctt tcattatgat ataacaaacg aaagtacaac ttcctacaat aacaatgtaa
     3241 tattcggact ttacatggat tccggtgtgg gaggttctgc attgggtcca aatggagttt
     3301 atgagtctga tgatgacaat gcattttttg ataagagtgc tggattgaat cttgtttata
     3361 cctgggataa ttatggaaac ggtcatattg gtcctacagg ttatcttggt tactcttact
     3421 tagaaacgcc tggtaatgct gtcgatggta ttgataatga tgaagatgga attttggatg
     3481 agagaagaga tagcggtccc ggacaacaaa ttataggaca agatgcaata ctaaattacg
     3541 taacagctaa ttatgattta gcaaaatttg aggcttataa tggagcaatc accgatagac
     3601 cagcttttac tgaaggtgtt tggtggacag gtgatgagga tcttgattgg gtagctgaat
     3661 attatgatac cggtgcagat ggaatctttg gcacaaacga tacaggtgag aaagatggac
     3721 aaccgacgaa tggtgaaacc aattttgata aaactgatat agatgaatct gatcagattg
     3781 ggctaaccgg atttaagtta aatcgaatta aagcaggagc aggtaatcca agtacagttg
     3841 ttgatcaaat agtatttttt gatgatggaa aacaatggcc taagaaatta tataatattt
     3901 ttacaaaccc tgatagtgcc tttggcgcac cggaagcact gaactataac atcggttttc
     3961 ttttcgcatc agggcccttt tatttggggg caggaaatcg tgaaagattt agtcttgcac
     4021 taggttttgg tgcaaatctt agagagctaa gagttactac taaagttgtg cagcagattt
     4081 ataaagctaa ttatcaattt gctattccgc cgccaatgcc aacagtaaat gcatttaccg
     4141 gggatggatt tgtaacttta acctgggata atgcaggtga acgtgcattc gatccaataa
     4201 ccaataataa tgactttgaa ggatatcgtg tataccgaag tacagacccc gcatttttag
     4261 atccgcaggt tatttataca gcaacaggaa ccagaccatt tgccagtaaa ccaatagcac
     4321 aatatgattt gataaacgga atcagcggat tttcaactac aactgttgaa ggtattgcat
     4381 attttcttgg agaagatacc ggtattaaac acacatttac tgatacgact gtagtaaatg
     4441 gacaaagata ttattatgct gtagtttcat acgatcgtgg tgttgattct tcgcagatat
     4501 acccttctga gaatgcaatt actattagtc aaacattacg cggcggcact atactccctc
     4561 aaaatgctgt ggaagtttat ccaaatccac cttcagttgg atttcaggat gcacaaactt
     4621 tgaatcttgt acaaagtgag ggaagaggct caggagaagt tagcttgcaa gttctaaatt
     4681 cagctatcgt tcctgaaaat catacctttg ctattaaatt tgataatgct tcggatagtg
     4741 ttcgcgcaga atcatattat ttagttgatg aaacacttag cgatacttta tttaccttag
     4801 gtgttgattt cgatggattg ggggcaggcc cttcgggggg tggaatactt cctattattt
     4861 acactccttt agtaactgaa attgacagtc agcatactgg attagtttca ggaagtggaa
     4921 caaatattcc tcttaaaatt agatactctg atgtactgcc aataaattta aagagacctg
     4981 gatttcctga aaacatagat gtgatattta gtgatatagt tattgataca tcactagctg
     5041 atccggtgaa cttcattcct gcaagacctg ttaagtttac agtaattgca aaaaccgaag
     5101 cgggtgatca gaaattaaaa tttacctttt atggtggcag caacagcact aatggaattt
     5161 tagatgctca tggtgattat atttcaattc ttactgcagc aactgaaacc ccaacacagc
     5221 gtaaaccaac ctggagatta gaagtagatt cgacaggttt aaatggtgct ccaattgtac
     5281 ccccaacaat caatgataaa tatgaactga gattaatatt cccatactcc agtcaggata
     5341 aatttacatt ccttacaaaa gcgcagtaca tagatcctaa tcttgcaaaa cagcaattcg
     5401 aaaaggaccc gtatgtagtg ccaaaccctt acgtaggtgc tgcaagcttt gaacctcaaa
     5461 gatttgctgt atcaggcaga ggcgagcgta aaattgaatt cagaaatctt ccggcaaatt
     5521 gcacaatcag aatttatact gtaacaggag aatttgtgaa agaattagct catgatggaa
     5581 atattaatga aggaattgta tcgtgggatt tacgcaactc aagcaatctg gaaattgccc
     5641 ccgggcttta tatatactac gttgatggtc caggtgttgg cacttttatc ggcaaatttg
     5701 caataattaa atgatgaagg tggaagaatg aaaaaattaa tacgaataaa tataaaagtt
     5761 gtttcaacct gcttccttat cgttttttta tttgcaggac aaaactttgg acaaaacaaa
     5821 accggcacaa caatcggtca atttttaaag attgaaccaa gttcgagagt ggtcgcattg
     5881 ggtaatgccg gtgcatcact ttcgggtgaa atctctaaca tcttttacaa cccggcaagt
     5941 ttaggaagac tgcaaggtgc tgatattcaa tttacattta ataaatggct tgctgatata
     6001 acttataatt atatggccgc tggaattaat gttgaaggtg ttgggacctt tgcattagtg
     6061 ggaacattac taaattccgg tgaaatggat gtcagaacag ttgaaagtcc attgggaaca
     6121 ggtgaaagat ttactgtaaa taatcttgct ctagggttag gatacggttt gatgctgaca
     6181 gacagagttt ctgttggtat tcaaattaat tatataaatg aaactatttg gcatagctca
     6241 ttaagcacct tcggattaaa ctttggagtt caatatcagg ttgttgaggg cggattaact
     6301 cttggtgcaa gtgtatctaa ctttggaaca agcgcagctt atgatggaag agatctttat
     6361 ctggattatg attttgatcc agataaatat ggtgataatg ataaacttcc ggcagaactt
     6421 agaactgata attttaatct tcccacatta ttcagagccg gtgtttcata ctggtttgat
     6481 tttggtgaga actacaaatt acttgtagct gcagatgcag tgcatccgaa tgataatgat
     6541 caatatttaa atctcggagc tgaatttaat ttgctcaaaa ttttttcgat tcgagccggg
     6601 taccgaaatt ggttaatgcc tgaagcagaa ggcgggttag tcctgggcgg tggagttcaa
     6661 acaaatttca atgaaacaaa taatattcga tttgattatg cttatgctga ttatggcaga
     6721 ttagctgaaa cacatcgcat tactttaagc gttggattta gataatataa atggaacggt
     6781 atatgaagca atactttgaa atgaagacaa ttaatacaat tctccttgtt gtctttctgt
     6841 ttgttggatg taaggctcag caagatcaga ttgtctttaa atcaaatgga aaagttgact
     6901 atcctctctc aaatagtgat gaaaaacttt tagattcaat tcaataccgt tcatttcttt
     6961 tttttaaaga gcagtcaaat cctgaaaatg gattagtaaa ggacagaagt gcaagctggg
     7021 ctcccgccag cattgcggca attgggtttg cattgccatc atacgctgtt ggtgttgaac
     7081 gaaattggat gtcccgtgat gaagctgcaa tgattactct taataccctg aacttctttt
     7141 taaactcagt tcaaagttct gatacaaatg caactggtta tagaggattt tattatcact
     7201 ttttaagaat gaactcagga acgcgcgaat g
//
LOCUS       DASWBY010000844         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_110449, whole genome shotgun sequence.
ACCESSION   DASWBY010000844 DASWBY010000000
VERSION     DASWBY010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110449"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1388)
                     /locus_tag="VF870_15270"
     CDS             complement(78..1388)
                     /locus_tag="VF870_15270"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9253605.1"
                     /translation="MKKILLLSVLYSLAVFAQVPIPETGCYHAAFTGNDSHASFESLA
                     GKNIAIEMFFTGWPSNMVPDFPLAKCTQIDNNGAVPHITWMPQVPGSPYPLNSIINGS
                     YDNYINGYAAQVKNWGKPLFIRLGHEFNGDWYTYGGANNGGGTLNGFGDPTKADGPER
                     FIAAYQRVHDLFEAQNVDNVVWVWCPNNGSTPNEAWNVPEAYYPGDDYVDWIGFDGYN
                     FGTSQTWSGWTTFFNVFNSLYTKFKNYGKPIMLGEFASVEEGGSKSQWITDGYLTWLK
                     FGYPKVKAATWFHVAKTEGTVYTDWRINSSAASLAAYQNAIADPYFLAAVPATDIKDE
                     KDIPEFFTLEQNYPNPFNPSTKIKWQSPFGSWQILKVYDILGNEVATLVNEYKPAGSY
                     EVEFNIGSDIKHLPAGNQGPASGIYFYRLQAGEYTATKKMILQK"
BASE COUNT          565 a          348 c          282 g          570 t
ORIGIN      
        1 tgcataaacg actcgaatac atgcaacgaa tctctgtgct tgtgattttt acctcttact
       61 tctggttaat tgccatatca tttctgtaaa atcatctttt tcgttgcagt atattccccg
      121 gcctgtaacc gatagaaata aatgccggat gctggaccct gattaccggc aggcagatgc
      181 tttatatcag atccaatatt aaattctacc tcgtatgagc cggcaggttt atattcgtta
      241 acaagagttg caacttcatt tcctaaaatg tcatatactt ttagaatttg ccaactgccg
      301 aatggggatt gccattttat ttttgttgaa ggattaaatg gattggggta gttttgttca
      361 agtgtaaaaa attccggaat atctttttca tcttttatat ctgtagccgg aacagcggcc
      421 agaaaatatg gatctgcaat tgcattttga tatgcagcta aagatgcggc tgaagaatta
      481 attctccaat ctgtataaac tgtaccttct gtttttgcaa catgaaacca cgttgcagct
      541 ttaacttttg gataaccaaa cttaagccag gtcagatatc catctgtaat ccattgactt
      601 tttgatccgc cttcctcaac tgaagcaaac tcgccaagca taatgggctt gccataattt
      661 ttaaattttg tataaagaga attaaaaaca ttaaagaatg ttgtccatcc tgaccaggtt
      721 tgagaagtgc caaaattgta tccatcaaaa ccaatccaat caacataatc gtcgccggga
      781 taatacgctt caggaacatt ccaagcttca ttcggagtac ttccgttatt cggacaccat
      841 acccaaacca cattatcaac attttgggct tcaaataaat cgtggactct ctgatatgct
      901 gcaataaatc tttcaggccc atctgcttta gtcggatcac caaatccatt taaagtacca
      961 ccgccattat ttgcaccgcc gtatgtgtac caatccccgt taaactcgtg ccctaatctt
     1021 ataaaaaggg gcttacccca atttttaact tgagctgcat atccatttat ataattatcg
     1081 taagaaccat taatgattga atttaaagga tatggactac cgggtacctg cggcatccag
     1141 gtgatatgcg gaactgcacc attattatct atctgcgtac acttggctaa aggaaaatcc
     1201 ggaaccatat tgctcggcca tccggtaaag aacatttcta tagcaatatt ttttcctgct
     1261 aaagattcaa agctagcgtg agaatcattt cctgtaaatg ctgcgtgata gcatcctgtt
     1321 tcaggaattg gtacttgtgc aaatacagca agtgaataca aaactgataa caataatatt
     1381 tttttcattt gaaatccttt tttcaaaaac taataatgca acaataaagc cagctttaaa
     1441 caaatcaata aatgggtttt tacttgttaa ttctatttta ctatgaacct attaaagttt
     1501 aactataatt taattattaa aataataatt tttttttatc aggttcataa ttcatttcaa
     1561 aatcattgcg attccgcttc tcaggagaaa caacctgcta ttagacttat ttcgaaaaaa
     1621 aagtgaattc tattattgaa aagcaataat taaaataaaa aacggaacga acgggagttc
     1681 gtttcctgca taattagatt cgcatctcac atcaaatctt cttaagtttc aataaataaa
     1741 aatgtctttt gggatacgac caaaa
//
LOCUS       DASWBY010000845         3957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_561527, whole genome shotgun sequence.
ACCESSION   DASWBY010000845 DASWBY010000000
VERSION     DASWBY010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561527"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VF870_15275"
     CDS             complement(<1..518)
                     /locus_tag="VF870_15275"
                     /inference="COORDINATES: protein motif:HMM:NF013246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HEX9253606.1"
                     /translation="MIVKRNFSPLKVWSYIKGPVILAIIWSFAVWLIANNIAAENFSL
                     NFSLVGILGTALAIFIAFKNNSSYSRWWEARTAWSSIQSSCRTFARLIITFTDSHSHQ
                     TNYVKERSESFKQEMIHKIIVWANSLRMELRNQNDWVSLQSFLTVEEMTQLKTKHNKS
                     NFIKLLIGQRIY"
     gene            complement(515..1279)
                     /locus_tag="VF870_15280"
     CDS             complement(515..1279)
                     /locus_tag="VF870_15280"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEX9253607.1"
                     /translation="MKSLNIFIAVFIFGQITYAQLTESDTTKFSWKLSSNGSWLTGNV
                     SRLLILSSADLTIIQEPWAIRFSNTYQYGTVSGKLTENDLFSRNFFYLFPRNEVYPYL
                     MGWIETNKRRNFSFRYQIGPGFSWGFVNNKSSSVKISFTATYEMTNYWNSVYSDEKYN
                     GENTIDLLRGTVRLFGKHKLFENKINIAYEAWGQQAFDDKENKRFYGNIVLEFPLTQL
                     LSFRTGYNYYYESLVLSGVKPQDGFLFFGISIGENK"
     gene            complement(1324..2070)
                     /locus_tag="VF870_15285"
     CDS             complement(1324..2070)
                     /locus_tag="VF870_15285"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX9253608.1"
                     /translation="MSANIDVFNSEKRKRILIIAANPSVSKQTGWPIGFWAAELTHPY
                     FEFTEKGYEVEIVSPDGGKLELDGFSDPKHESGYSAHDLISLGFLSSPNHSKLLENTK
                     SISQVKIEEYDALFLAGGQSPMYTFVDNTKLHKFVSDFYEAGKVVAIVCHATCVLLKA
                     KTKDGKLLVDGKTWTGFANSEEQFADNFVGMKIQPFWIEEEAKKLPNTNFIVNGMFKP
                     FAVRDGRLITGQQQYSGGAAAKLVIETLGV"
     gene            2214..2549
                     /locus_tag="VF870_15290"
     CDS             2214..2549
                     /locus_tag="VF870_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9253609.1"
                     /translation="MPRFEFRGKYYNNPVELSLDIIGGKWKMPILWRLKERSWRYGEL
                     KKNIGKITHKMLAEQLKELEQDGLISRKVYPEVPPKVEYSLTERGFSTIPIIEGLRAW
                     GSSFRENKI"
     gene            complement(2546..>3957)
                     /locus_tag="VF870_15295"
     CDS             complement(2546..>3957)
                     /locus_tag="VF870_15295"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9253610.1"
                     /translation="QSDKISQIFASLNGDHFSGKQVLAKVIQLIELYREEGYSFAEIQ
                     SIEYNPEEKRLKIYIDEGLISRIDVIGNKYTNSSVITREFSFEAGDVFQVKEVQKGLT
                     NLRSTKLFDNIDVLVKEENGQNILIISVDEKPSSLLRISFRTDNEYRAQFGLDIRDEN
                     LFGTGTELGLILFGGLENRAYTLEQKANRIFDTYFTYKINAFYKFNDVRVYSDVETGS
                     DDNFSREETGRYRQIFYGFSLGLGTQVGRFGNLIFEGRYQFDEIKNIDNEPVNPYKTK
                     IVSLKISTTIDTQDKYPFTENGVYFNGFYETAATVLGGEVGYSNISFEYKSYFSISSR
                     SVISPKISFGFADKTLPLSEQYSLGGQDSFFGMRNNEFRGRQIFLASLMYRYKLPFII
                     FFDTYLKVRYDLGSTWEQQEQIRFKDLRHGVGGAISFDTPIGPAEFAVGRSFLFKKDQ
                     PGVVSWGDVLFYFSIGYYY"
BASE COUNT         1257 a          746 c          608 g         1346 t
ORIGIN      
        1 ttataaattc tttgaccgat taacagcttt atgaaatttg atttgttgtg tttcgttttt
       61 agttgtgtca tttcttctac agtcaaaaag gattgaagac ttacccagtc attttgattt
      121 ctcaattcca ttctcaatga atttgcccag acaataattt tatgaatcat ctcttgctta
      181 aaagattcgc ttctttcctt cacatagttg gtttgatgag aatggctgtc tgtaaatgta
      241 ataatcaatc ttgcaaaagt tctgcaagag ctttggatac tgctccaggc tgttcgggct
      301 tcccaccatc gtgaatatga agagttattt ttaaatgcaa taaatattgc aagagcggtt
      361 cctaaaattc caaccaatga aaaattcaaa ctgaaatttt ctgcagcaat gttgtttgca
      421 atcaaccaaa ctgcaaatga ccaaattata gctaagataa caggaccttt tatgtagctc
      481 catactttta atggactaaa atttcttttt acgatcattt attttctcca atagaaattc
      541 caaagaaaag aaatccatcc tgcggtttca ctcccgaaag tacaaggctt tcgtagtagt
      601 aattatatcc tgttctgaat gaaagtaatt gtgttaatgg gaattcaaga acaatatttc
      661 catagaatct tttattctct ttatcatcaa aagcttgctg tccccatgcc tcatatgcta
      721 tatttatttt attttcaaat aacttatgct taccaaaaag cctgacagtt cctcttaaca
      781 aatctatagt gttctcgcca ttatattttt catcagaata aactgaattc cagtagtttg
      841 tcatctcata cgttgctgta aatgaaattt taactgatga ggatttatta ttaacaaatc
      901 cccagctaaa gcctggtcca atttgatagc gaaatgaaaa gtttctacgc ttattcgttt
      961 ctatccatcc catcagatat ggataaactt catttcttgg gaaaagatag aaaaaatttc
     1021 ttgagaatag atcattttcc gttagcttac cactcaccgt tccgtactga tatgtatttg
     1081 aaaatcttat cgcccaaggt tcttggatta ttgtaagatc agcagaacta agaattaata
     1141 atcttgagac attacccgtt aaccatgatc cgttactact taatttccag ctaaattttg
     1201 ttgtgtcact ttcggttaat tgtgcataag ttatctgtcc aaaaatgaat actgcaataa
     1261 agatgttcag tgatttcatc ataataataa agccccgatt caataatcgg ggcgtgttag
     1321 aaactaaaca cctaaagtct ctattactaa ttttgcagct gcgcctccgg aatactgctg
     1381 ctgaccggta attaatcttc catctctaac tgcgaatggc ttaaacattc cattgactat
     1441 aaagtttgta tttggaagtt tctttgcttc ctcttcaatc cagaatggct gaattttcat
     1501 tccaacaaaa ttatcagcaa actgttcctc actattagca aaccctgtcc aagtttttcc
     1561 atcaactaaa agtttaccgt cttttgtttt tgcttttagg agtacacagg tagcatgaca
     1621 aactattgcg acaacctttc ctgcttcata aaaatctgaa acaaatttgt gaagtttagt
     1681 attatcaacg aaagtataca taggtgattg gccgcccgct aaaaacagag catcatattc
     1741 ttcaatcttc acctgagaga tactttttgt gttttctagt aattttgagt gatttggtga
     1801 acttagaaat cccaagctaa tcagatcgtg cgcagaataa ccactttcat gttttggatc
     1861 gctgaaacca tcaagctcaa gttttccacc gtctggacta acaatctcaa cttcataccc
     1921 tttttcagta aactcaaaat aaggatgcgt taattctgca gcccagaatc caatcggcca
     1981 gccagtttgc tttgatactg atggatttgc tgcgataatt aatattcttt ttcttttttc
     2041 cgaattaaaa acatctatgt ttgcactcat aatttttcct ttcaaaaata tttaatagtt
     2101 ttatgtcaag atatttttga tatttaattg tatcaagaac gcacaaaaaa gtagcatact
     2161 tactaactag taagaaatga aatttcattt aaaactttac agggtaaatt tttatgccca
     2221 gatttgaatt ccgaggaaag tattataaca atccagttga actctcgctt gatataattg
     2281 gcggcaagtg gaagatgccg attttatgga ggttaaaaga aagaagctgg agatacggag
     2341 agctgaagaa aaacattggc aaaataactc ataaaatgct tgctgaacaa ttaaaagaac
     2401 ttgaacaaga tggattgatt tctcgaaaag tttatccaga ggttcctccc aaagttgaat
     2461 actctttaac tgaaagaggt ttttccacaa taccaataat agagggttta cgagcatggg
     2521 gaagcagctt cagagaaaat aaaatttagt aataatatcc aatagaaaaa tagaacagaa
     2581 catcgcccca gctaactaca ccaggttgat ctttcttaaa taaaaaactt cgtccaactg
     2641 caaactctgc tggcccaatt ggcgtatcaa atgaaattgc tccaccaaca ccatgtctaa
     2701 gatctttaaa tcttatttgt tcctgttgtt cccatgtaga tcctaaatca tatcttactt
     2761 ttaaataagt atcaaagaaa attatgaatg gaagtttata tcggtacatt aaagaggcaa
     2821 gaaatatttg tcttcctcta aactcattgt tacgcatgcc aaaaaaggaa tcttgtccgc
     2881 caagcgagta ttgttcgcta agaggcagtg ttttatctgc aaaaccaaag cttatttttg
     2941 gtgatataac agaacggctt gatatggaaa aataactttt gtattcgaag ctgatgttac
     3001 tataaccaac ttccccgcct aaaacagttg ccgctgtttc ataaaagcca ttaaaataaa
     3061 ctccgttttc cgtaaaggga tatttgtctt gagtatcaat tgttgttgaa atttttaaac
     3121 ttacaatttt cgttttatat ggatttaccg gttcgttgtc tatgttttta atttcatcaa
     3181 actgatatct gccttcaaaa attaaattac caaaacgacc aacctgtgtt ccaaggccaa
     3241 gcgaaaaccc gtaaaatatt tgtctgtacc ttccagtttc ttctctggaa aaattatcat
     3301 cgcttccagt ctcaacatca gagtatacgc gcacatcatt aaatttgtaa aaagcattga
     3361 ttttataagt aaaataagtg tcaaaaattc tgttagcctt ttgctcaagt gtatatgctc
     3421 tgttttcaag tcccccgaat aatattaatc caagctctgt tccggtaccg aaaagatttt
     3481 catctcttat atccaaacca aattgtgccc ggtattcatt gtcggttcta aaactaattc
     3541 taagcaggct tgatggtttt tcatcaacac tgataatgag aatgttttgt ccgttttctt
     3601 cttttacgag aacatcaata ttatcaaaca gttttgtgct tctgagattc gtcaatcctt
     3661 tttgcacttc tttaacctga aaaacatccc ctgcttcaaa actaaattca cgagttataa
     3721 cactgctgtt tgtatatttg tttccgataa catcgatacg agagattaac ccttcatcaa
     3781 tataaatttt aagtcttttt tcttccggat tatattctat gctttgaatt tctgcaaagg
     3841 aataaccttc ctcacgataa agctctataa gttgaattac ttttgccaga acttgtttcc
     3901 cggaaaaatg atctccgttt agtgaagcga atatctggct tattttatcg gattgaa
//
LOCUS       DASWBY010000846         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_187043, whole genome shotgun sequence.
ACCESSION   DASWBY010000846 DASWBY010000000
VERSION     DASWBY010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187043"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VF870_15300"
     CDS             <1..1065
                     /locus_tag="VF870_15300"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX9253611.1"
                     /translation="NGVVIDPTALLEEIELLKQNNIDVDGRLFISHNAHLIMPYHKLL
                     DSINESGNNKIGTTGRGIGPCYIDKYARKGIRIVDLLNRTELEKKIRANLKEKNEILQ
                     KIHSHEGLDVDAIVKEYLEFDLTIDKYIKDAPAFLNRAMDEGKSVLLEGAQGTFLDID
                     HGTYPYVTSSNPTSGGACTGSGIPPTRIDSVIGIVKAYTTRVGNGPFPTELLEDDGEK
                     LRKIGAEFGATTGRPRRCGWYDAFLVAYSKMINGITSVALTKLDVLSGFEKIKVCVGY
                     EVNGKKLKTFPTNVDQLMQVTPVYETLDGWNTDITGCENYDDLPAKTKDYLDFISRES
                     GIRIDIVSVGPKRKQTFYVE"
     gene            1152..2360
                     /locus_tag="VF870_15305"
     CDS             1152..2360
                     /locus_tag="VF870_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9253612.1"
                     /translation="MKMSGGPASVNFKIILLLIALAIAGGTLYYTQTLVTKLQQRERN
                     IVELYAKGIEYVANTNSPDVDLTFLFDNIIKPIDFPIILTNSNDKVNLSGSSDVKNIK
                     YDSTLSKTEIKSFLENYVKEMDEIHQPINVSYADTIILQRIHYGDSELIKQLKFYPYL
                     QIIIAALFIIIGYIGFSQIKRSEQSNIWVGMAKETAHQFGTPISSLMGWIEMMKMNYQ
                     KPDAVLDITDEISNDVDKLNRITYRFSKIGAKPEIKNHNVYEEVKKVTEYFNRRLPQT
                     GKTVELNVKGDQTCSAQMNSELFEWVMENLIKNALDAIETKNGQININIADAKKNIEI
                     EVSDNGKGIDLRRRKDVFRPGYSTKKRGWGLGLSLSKRIVEGYHGGKIFVKNSAPGEG
                     TTFKIMLKKS"
     gene            complement(2357..>3127)
                     /gene="nadA"
                     /locus_tag="VF870_15310"
     CDS             complement(2357..>3127)
                     /gene="nadA"
                     /locus_tag="VF870_15310"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX9253613.1"
                     /translation="DVIVFAGVHFMAETAKILNPDKLVLLPDLEAGCSLAEGCPADEF
                     KLFREKHPDHLAITYINCSADVKALSDIICTSSSAEKIINQIPEDQPILFSPDRNLGK
                     YLIKKTGREMLLWDGSCIVHETFSERKIIDLKIKYPNAKLISHPECEEPILMHSDFIG
                     STSRLLSFVQEDSAPQFIVATETGIIHQMKKAAPEKEYIPAPPEDDTCSCNECPYMRL
                     NTLDKLYLCMKNQYPEITMDDEIRKKALLPIERMLAMS"
BASE COUNT         1079 a          507 c          567 g          974 t
ORIGIN      
        1 aacggtgttg ttatagatcc caccgccctg ctggaagaaa ttgaacttct aaaacaaaat
       61 aatattgatg ttgacggtcg attatttata agtcacaatg ctcaccttat tatgccatac
      121 cacaaacttc tcgattcaat taatgaaagc ggtaataata aaatcgggac aaccggtaga
      181 ggaattggcc cctgctatat tgataagtat gcaagaaaag gaattagaat tgttgatctt
      241 ttaaatcgga ccgaattaga aaaaaaaatt cgcgcaaatc ttaaagaaaa aaatgagata
      301 ctacaaaaaa ttcatagtca cgaaggcctt gatgtagatg caattgtaaa agaatatctt
      361 gagtttgatc ttacaataga taaatatatt aaagatgctc ctgcattctt aaatcgtgct
      421 atggatgaag gaaaatctgt tttacttgaa ggcgcacagg gtacttttct ggatatcgat
      481 catgggacct atccttacgt tacatcaagc aatccaactt ctggtggagc ctgtaccgga
      541 agcgggatcc ctccaacaag aattgatagt gtaattggaa ttgtaaaagc atatactaca
      601 agagttggca atggtccgtt cccaaccgaa ttgctcgagg atgacggtga aaaacttaga
      661 aagattggtg ctgaatttgg tgccacaacc ggaagaccaa gacgttgtgg atggtacgat
      721 gcttttttag ttgcttattc caaaatgatt aatggaataa cttctgttgc cttaaccaag
      781 ttagatgttt taagcggatt tgaaaaaatt aaagtatgtg ttggttatga ggttaatggc
      841 aaaaaattaa aaacatttcc aacaaatgtg gatcagctaa tgcaagttac acctgtttat
      901 gaaacacttg atggatggaa tacggacatt accggctgcg aaaattatga tgatcttcct
      961 gcaaaaacaa aagactattt agattttatt tcaagagaat caggaatccg gatagatatt
     1021 gtttctgttg gtccaaagcg taaacaaact ttttacgttg aataaaaacg aattagagat
     1081 ttattttaag ctggattttg cttcctgata atacaatttt tgcctatcaa taacatcaga
     1141 ttgaatagac tatgaaaatg tccggcggtc ctgcttctgt aaatttcaaa attattctcc
     1201 tgttaattgc attagcaatt gccggaggaa ccctctacta cacacaaaca cttgtaacaa
     1261 aactccagca gcgcgaacga aacattgttg aactttatgc taaagggata gagtatgttg
     1321 caaacactaa ttcccctgat gttgacttaa catttctttt tgacaacata ataaagccaa
     1381 tagatttccc tataattctg actaatagca atgataaagt taatctttca ggcagttccg
     1441 atgttaaaaa tattaagtat gattctacac tatccaaaac tgaaataaaa tcttttcttg
     1501 agaattatgt aaaagagatg gatgaaatcc atcagcctat aaatgttagt tatgctgata
     1561 caattatttt gcaaagaatt cattacggcg attccgagct tataaaacag ttaaaatttt
     1621 atccttatct gcaaattata attgctgcac tttttataat tattggctac atcgggttca
     1681 gccagatcaa aaggagcgag caaagtaata tttgggtagg aatggcaaag gaaacagcgc
     1741 atcaatttgg tactcctatt tcaagtttga tgggatggat tgaaatgatg aaaatgaatt
     1801 atcaaaaacc tgatgccgtt ctggacataa ctgatgaaat ttctaatgac gttgataaac
     1861 tcaatagaat tacatataga ttttctaaaa tcggtgctaa accagaaatt aaaaatcata
     1921 atgtttatga agaagtaaaa aaagttactg aatactttaa tcgcagactg cctcaaacag
     1981 gtaaaactgt tgagctgaat gtaaaaggtg atcaaacctg ttctgcacag atgaatagtg
     2041 aattatttga atgggttatg gaaaatctta ttaaaaatgc tttagacgcc atcgaaacta
     2101 aaaatgggca aattaatatc aatatcgctg atgctaagaa aaatatcgaa atagaagttt
     2161 ctgataatgg aaaaggtatt gatctacgaa ggcgaaagga tgtttttaga cctggatata
     2221 gtactaaaaa acgaggttgg ggtttaggtt taagtctttc taaaagaatt gtggaaggct
     2281 accacggagg taaaatattt gtgaagaatt ctgctccggg agaaggaact acttttaaaa
     2341 taatgttaaa aaagagttag ctcattgcta acattctttc aattggtaat agtgcttttt
     2401 ttctaatttc atcatccata gtaatttctg gatattgatt tttcatacat aggtacaatt
     2461 tatctaaagt attcaatctc atgtaaggac attcattaca agaacaagta tcgtcttctg
     2521 gtggagcagg aatatattct ttttccggtg ccgctttttt catctggtga atgatcccag
     2581 tttcagtcgc aacaataaat tgaggcgcgg aatcttcctg cacaaaactt aataatctgc
     2641 ttgttgatcc aataaaatcc gaatgcatta aaattggttc ctcacattca gggtgtgaaa
     2701 tcagttttgc atttggatat tttattttaa gatcaattat ttttctttcg ctaaaagttt
     2761 catgaacaat gcaacttcca tcccatagca gcatttccct gcccgtcttt ttaatcagat
     2821 attttccaag atttctatca ggagaaaaaa gtattggctg atcttcagga atttgattaa
     2881 taattttttc agcactgctg gatgtacaaa ttatatcgct caatgctttt acatccgcag
     2941 aacaattaat gtatgttatt gcaagatgat ctggatgttt ttcacgaaac aacttaaatt
     3001 catctgccgg acaaccctca gctaaagagc agccagcttc taaatcaggt agcaaaacta
     3061 atttatctgg atttaatatc tttgctgttt cagccataaa gtgaacacca gcaaaaacga
     3121 ttacatc
//
LOCUS       DASWBY010000847         6754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_750457, whole genome shotgun sequence.
ACCESSION   DASWBY010000847 DASWBY010000000
VERSION     DASWBY010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750457"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VF870_15315"
     CDS             complement(<1..763)
                     /locus_tag="VF870_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX9253614.1"
                     /translation="MKEDTIVALATPAGVGAISIIRVSGPQSFSAVDNIYFGKSKIEN
                     AATHTLHYGNIKNQDDEHIDDVLVSVFRAPNSYTGEDSVEISTHGNPLITQKVIELLI
                     TSSDVRLAEPGEFTKRAFLNNRIDLTKAEAVADVINSRTEASFRGARNQLDGLLSKKV
                     NELRYQLLNSSSLVELELDFVEEDIQFVNQDELLKRIDSIIIEIDTLLESYEFGRVIR
                     DGVNVAIVGRPNVGKSSLLNYILKESRAIVSEIPGT"
     gene            1071..1535
                     /locus_tag="VF870_15320"
     CDS             1071..1535
                     /locus_tag="VF870_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9253615.1"
                     /translation="MTLVKFNPVRDLIDFEREFNKMFSSLENRFGISKSKEEDTEYEN
                     AVWMPLTDIYEDKDKYSLKLDLPGIKKEDVKISYVDGRLSIGGERTQEVEHKDSKCHR
                     IERSFGKYYRSFNLPEHIQIEKINAEFKDGQLTITVPKAEEAKPKEIEIKVK"
     gene            1632..2570
                     /locus_tag="VF870_15325"
     CDS             1632..2570
                     /locus_tag="VF870_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX9253616.1"
                     /translation="MEYKDYYKILGVEKSATQDEIKKAYRKLAMKYHPDRNAGNKSSE
                     EKFKEITEANEVLSDPEKRKKYDTLGANWKQYQHTGGQGFDDFFTNFGGGRKRSSGST
                     YEFSGNINDIFGDMGGGGFSDFFESFFGGGGRGFGGRTQQQNTAVDVEANMNVMLEDV
                     FNGSEKQISVDSKIIKIKINPGTKDGQRLRLKGMGRSKTADGTKGDLFLNIHILRHPF
                     YEIKDGTLFYNLDVDLYTAVLGGKENIKTLDGKTVSINIPEGTESEKKLRLKGLGLIE
                     NGVRGDLLVDIHVTVPKDLTKNEKELFKKLKSLRPE"
     gene            2672..4585
                     /gene="htpG"
                     /locus_tag="VF870_15330"
     CDS             2672..4585
                     /gene="htpG"
                     /locus_tag="VF870_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX9253617.1"
                     /translation="MTETHTTKFEFKAETKKLLDILVHSLYTSKDIFLRELISNSSDA
                     LDKLRFESNKGTDIADKDLPLEIKIGFDDKKNTITISDTGIGMTRDEMIANIGTIAKS
                     GSEEFLKQLSENKDAINNIIGRFGIGFYSVFMVAKEVIIKTKSYKKDEVAVEWKSDGL
                     GDYEISDIDEQINRGTTIEIFLKEEAKEFSEKYRLESIIKKHSNFISFPIYLENEKIN
                     TIAAIWREPKSNLKKEQYDEFYKFLTYDNEEPLETIHSSVDAPIQFNSLLFIPKKSYE
                     YWRFNREDYGLDLYVRRVLIQHQNKDLLPEYLSFVKGVVDSEDLPLNISRETLQENVI
                     FTKISQSVTGNVLTHLNKVAKDSPERYADFWKEHGKIFKLGYMDFTNAEKYQQLLRFN
                     SSVSKDEKELVSLEDYVGRMKKDQKEIYYSLGTSKEAIDLNPHLEIFKAKGLEVLYLY
                     DPVDEFVVSSIRKHKDFDFKSVDGVNLKDLDKLEDVVKDEEPKEKFSKEDDKAFGKLM
                     LKMKEILGDRVTEVHESKRLKGSPSCLINPDDTMSSTMQKILKMSNQGMAMPPQKRLM
                     EINKDHKLIRNLMNVFKKNESDQFIVDTTEQLYESALLLEGNLDDPHKLVNRLNKMLT
                     EASELYASSKNDK"
     gene            4655..>6754
                     /locus_tag="VF870_15335"
     CDS             4655..>6754
                     /locus_tag="VF870_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9253618.1"
                     /translation="MSFNFNRLTVKAQEILQSAIEIAQNYNNQIVEPEHLLAAIVQES
                     GNVAESIIKKTGGNFNAVKLKINQLLESLPKVSGTGLGNQQMGQNLGKLFDTAAEEAR
                     NLKDEYVSTEHILLSLANDKGKTGQLLRDNGITYNDLLAALKTVRGTQRVTSQNPEDT
                     YQSLEKYGRDLNDLVKQGKLDPVIGRDEEIRRVLQVLSRRTKNNPVLIGEPGVGKTAI
                     AEGIAHRIVAGDVPENLKTKRIVALDMGALIAGTQFRGQFEERLKAVIKEVQESNGEI
                     ILFIDELHTLVGAGATQGAMDAANILKPALARGELHAIGATTLDEYKKHIEKDAALER
                     RFQPVLVDEPSEEDTISILRGLKERYEVHHGVRITDGAIVAATQLSERYITDRFLPDK
                     AIDLIDEAASKLRIEIDSMPEELDTLERKVKQLEIEREALKREKDEASAKRLEELEQE
                     LSELNEERTQLRLHWDLEKEKIQKIRSMKSEIENLKVEAERFEREGNLGKVAEIRYGK
                     ITELEKKLKEETQRLAEAQKDKKMLKEEVDAEDIAEIVSKWTGIPVNKMLESEKSKLL
                     RLEDELHQRVIGQDEAVSAVSNAIRRSRSGLQDSNRPIGSFIFLGTTGVGKTELARAL
                     AEVLFDDEHAMIRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLL
                     DEIEKAHSDVFNVLLQVL"
BASE COUNT         2413 a         1066 c         1335 g         1940 t
ORIGIN      
        1 tagttcctgg aatttcgctt acaatggcac gtgattcttt taggatataa ttcagaagtg
       61 atgacttccc tacgtttggt ctgccgacaa ttgccacatt tacaccatct cgaataactc
      121 taccaaattc gtaactttcc agcaatgtat ctatttcaat aattatcgaa tcaattcgtt
      181 ttagcaattc atcctgattt acaaattgta tgtcctcttc aacaaaatcc aattctaatt
      241 caactaatga tgatgaatta agtaattgat atcttagttc atttactttt tttgataaca
      301 atccatcaag ctgatttctt gcacctcgaa atgaagcttc tgttctggag ttaattacat
      361 cagcaacagc ttcagctttt gtaagatcaa ttctattatt aagaaatgct cgcttagtaa
      421 actcgcctgg ttcagctaat cttacatcag aactggtaat caaaagttca atcacttttt
      481 gtgttatcaa cggatttcca tgcgtgctta tctctacact atcttcgcct gtgtatgaat
      541 ttggagccct gaaaacagac actaaaacat catcaatatg ttcatcatct tgatttttta
      601 tattaccata atgtaatgtg tgtgtagctg cattttcgat cttactcttg ccaaaataaa
      661 tattatccac agcagaaaac gattgagggc cgctaactct gataatcgaa attgcaccca
      721 ccccggctgg agtagcaagc gcaacgattg tatcttcttt cactttaata atgctcaaat
      781 tttaagagtg caaaaataat cattattgaa agggatatca attaaacaat aaatactgct
      841 ttttaatcga tgtttgtaat aatggcatat atttatgacg atattttata attatacaat
      901 tatatggcaa tatgtccgcc attttgtctt tttattcaca aatcaggttt aatacggtaa
      961 ctcattgttt tataatgggt tatggtgatt tttgttttcg gcacgctgat tggatatttg
     1021 aaatcgagtt taaaataaaa ttaacaacaa taaaaataag gagacataca atgacactag
     1081 taaaatttaa cccggtcaga gatcttattg atttcgaaag agaattcaat aaaatgttca
     1141 gttcacttga aaaccgattt ggtatttcaa agagcaaaga ggaagacacc gaatacgaaa
     1201 atgctgtgtg gatgccatta acagacatct atgaagacaa agataaatac agcctaaaac
     1261 tcgatcttcc aggaattaaa aaagaagatg taaagatttc ttatgttgat ggaagactta
     1321 gcattggtgg tgaaagaaca caggaagttg aacataagga ttctaagtgc cacagaattg
     1381 aaagatcttt tggaaaatat tacagatcat tcaatctccc agagcatatt cagatagaaa
     1441 agataaacgc tgaattcaaa gatggtcaac ttactattac ggttcctaaa gctgaagaag
     1501 caaaaccaaa agaaatcgaa atcaaagtga aataatataa gagtcgatga ttatatgaaa
     1561 agggtaactg tttggttacc cttttctttt atctaaattt caagtgagac tttctcttat
     1621 ttaggcttaa aatggaatac aaagattatt acaaaatact tggcgtagaa aaatccgcaa
     1681 cgcaggatga gattaaaaaa gcttaccgaa agcttgctat gaaatatcat cccgaccgta
     1741 atgccggtaa taaatcctct gaagaaaaat ttaaagaaat tacagaagcc aatgaagttt
     1801 taagcgatcc ggaaaaaaga aaaaagtatg atacccttgg tgcaaactgg aaacaatatc
     1861 agcataccgg cggacaaggc tttgatgatt tctttacaaa ctttggcggc ggaagaaaac
     1921 ggtcgtctgg ttctacttat gaattctcgg gaaacattaa tgacatattc ggtgatatgg
     1981 gtggtggtgg attttctgat ttctttgaat cattttttgg cggtggtgga agaggttttg
     2041 gaggtagaac tcaacagcaa aatactgcgg tagatgttga agcaaatatg aacgtaatgc
     2101 ttgaggatgt ttttaacggc agcgaaaaac aaatttctgt tgacagtaaa ataattaaga
     2161 tcaaaataaa tcccggaacc aaagacggac aaagattgcg tctaaaggga atgggaagat
     2221 ccaaaaccgc ggatggaact aaaggagatt tatttttaaa cattcatatt ctccggcacc
     2281 cattttatga aataaaagat ggtacattat tttataatct agatgttgat ctttacacag
     2341 ctgtgcttgg cggtaaagaa aatatcaaaa cattagatgg aaaaacagta agcattaata
     2401 ttcccgaagg aacagaatca gaaaagaaat taagacttaa gggacttggt ttgattgaaa
     2461 atggtgttcg tggtgattta cttgttgata ttcacgtaac tgttccaaag gatcttacta
     2521 aaaatgaaaa agaattattt aaaaaattaa aatcattaag accagaataa tgttattgcg
     2581 ggtcccgatt tataggaatg aagcagtctg atttgcatag attgcttctt cacttcattt
     2641 ctcgcaatga cttttaatta aaggagaaac tatgaccgaa acacacacaa caaaatttga
     2701 atttaaagct gagactaaaa agctattaga tattcttgtt cactcgcttt atacaagtaa
     2761 ggatattttt ctgcgcgaat taatttcaaa ttcctctgat gctttggata aacttcgctt
     2821 tgaatcaaac aaaggaactg atattgccga taaagactta ccattagaaa ttaaaatcgg
     2881 atttgatgat aagaaaaata caataaccat ttccgataca ggaattggaa tgactcgtga
     2941 tgaaatgatt gcaaatatcg gaacgattgc taaatccgga tcagaagaat ttttaaagca
     3001 actttcagaa aataaagatg ccattaataa cataatcgga agatttggta ttggtttcta
     3061 ctctgtgttt atggtggcaa aagaagtaat aatcaaaaca aaatcttaca aaaaagatga
     3121 ggttgctgtt gaatggaaat ctgatggact tggcgattat gaaatttctg atattgatga
     3181 acagataaat cgcgggacta caattgaaat atttcttaag gaggaagcaa aagagttttc
     3241 cgaaaaatat cgtttagaat caatcatcaa aaaacactct aactttattt cttttccaat
     3301 ctatttagag aatgaaaaga tcaacactat cgctgcaata tggcgtgaac caaaatctaa
     3361 tcttaaaaaa gaacagtatg atgagttcta caagttttta acttatgata atgaagaacc
     3421 gcttgaaacg attcactcct ccgttgatgc cccgatacag tttaacagct tgttattcat
     3481 tcctaaaaaa agctacgagt attggagatt taaccgtgaa gattacggat tggatttata
     3541 tgttcgtcgt gttctaattc agcatcaaaa caaagattta ttgcctgagt acttaagttt
     3601 tgttaagggt gttgttgatt ccgaagatct gccgcttaat atctccagag aaactttaca
     3661 ggagaatgta atctttacaa aaatttcgca aagcgttacc ggtaatgttc ttacgcacct
     3721 aaacaaagtt gcaaaagatt ctcctgaacg ttacgctgat ttttggaaag agcatggaaa
     3781 gatattcaag ttaggttata tggattttac caatgcggaa aaatatcagc agctactaag
     3841 atttaattct tccgtaagta aagatgaaaa agaactggtt tctcttgaag attacgttgg
     3901 aagaatgaaa aaagatcaaa aagaaattta ttactcgctt ggtacaagca aggaagcaat
     3961 cgatctcaat ccgcatcttg aaatttttaa ggctaaagga ttagaggttc tttatttata
     4021 cgatcctgtt gatgagtttg ttgtttcttc aattcgcaag cataaggatt ttgatttcaa
     4081 atctgttgat ggtgtaaatt taaaagatct agacaaactt gaagatgttg ttaaagacga
     4141 agagccaaaa gaaaaattca gcaaagaaga tgataaagct tttggcaagc ttatgttaaa
     4201 gatgaaagaa attctcggtg atagagttac agaagttcac gaatcaaagc gattaaaggg
     4261 cagcccatcc tgcctgataa atcctgatga tacaatgtca tcaacaatgc aaaagatttt
     4321 gaagatgtcg aatcagggaa tggcaatgcc tccgcaaaaa agattgatgg aaataaataa
     4381 ggatcataaa cttatcagaa atttgatgaa cgtatttaag aaaaatgaaa gcgatcagtt
     4441 tattgtggat acaacagagc agctttatga atcagcgttg ctgcttgaag gaaatttgga
     4501 cgatccacat aaattggtga acagactgaa taagatgtta acagaagcaa gtgagttata
     4561 tgcttcttca aagaatgata aatgatcagg attcgatgat aatttgtcac actgagcgaa
     4621 gtctaagtgt gaatttctag aataggaaaa taatatgtca tttaatttta atagactaac
     4681 agtaaaagcg caagaaattt tgcaaagtgc aattgagatt gcacagaact ataacaacca
     4741 gattgttgag ccggaacatc tgcttgctgc tattgtgcag gaaagcggaa acgttgctga
     4801 atcaataatt aaaaaaactg gtggtaactt caatgcagtt aagctaaaaa taaatcagct
     4861 gctcgaatct ttacccaaag taagtggaac tggattgggc aatcagcaaa tgggccagaa
     4921 tctgggaaag ctttttgata ctgccgcaga ggaagcgcgc aatcttaaag atgaatacgt
     4981 ttcaacagaa catattctcc tttcacttgc aaacgataaa ggaaaaacag gacaattact
     5041 ccgcgataat ggaattactt ataatgacct acttgctgca ctaaaaacag taagaggaac
     5101 acagcgagtt acttcacaaa accctgaaga tacttatcag tcgcttgaga aatatggaag
     5161 agatttgaac gatttggtca agcagggaaa acttgatcct gtaatcggca gagatgaaga
     5221 aataagaaga gtacttcagg tgctttcaag aaggacaaaa aataatcctg ttttaattgg
     5281 cgaacccgga gtggggaaga cagcaatagc cgaaggaatc gcacaccgta ttgttgccgg
     5341 tgacgttccc gaaaatttaa aaacaaaaag aattgttgcg cttgatatgg gtgcacttat
     5401 tgcgggaacg cagttccgag gacagtttga agagagatta aaagcggtta taaaagaagt
     5461 tcaggaatca aatggagaga tcattttatt tattgatgag cttcatactc ttgtaggtgc
     5521 aggtgcaact cagggtgcaa tggatgcagc aaatattttg aaacctgctc tcgctcgcgg
     5581 tgaacttcat gcaattggtg caacaacatt ggatgaatat aaaaagcaca tagaaaaaga
     5641 tgcggctctc gaaagaagat ttcaacccgt tcttgttgat gaaccttctg aagaagatac
     5701 aatttcaatc cttcgcggac tgaaagaacg ctacgaagtt catcatggtg ttcgcataac
     5761 tgatggagcc attgttgccg caactcaact ttctgaaaga tacataacag atagatttct
     5821 tcctgataaa gcaatcgatc tgatcgatga agcagcatca aaacttagaa ttgaaattga
     5881 ttcaatgccg gaagaacttg atactcttga acgaaaagta aaacagcttg agattgaacg
     5941 cgaagcattg aagagagaaa aagacgaagc ttccgcaaaa cggcttgaag agcttgaaca
     6001 ggaattgagc gaacttaatg aagagcggac tcagttaaga ttacattggg atcttgagaa
     6061 agaaaaaatc cagaagatca gatcaatgaa aagtgaaatc gaaaacctga aagtagaagc
     6121 tgaaagattt gagcgagaag gtaatctcgg caaggttgca gaaatccgtt atggaaaaat
     6181 aacagagctt gaaaagaaat taaaagaaga aactcaaagg cttgccgaag cacaaaaaga
     6241 taaaaagatg cttaaggaag aagttgatgc agaagatatt gccgagatag tttcaaagtg
     6301 gaccggaatt cctgttaaca aaatgcttga aagtgaaaaa agtaaacttt taagacttga
     6361 agatgaactt catcaacgtg tcattggaca ggatgaggct gtatctgcag tttcaaatgc
     6421 aattcgaaga tcacgctcag gtttgcagga ttcaaatcgc ccgattggtt catttatatt
     6481 tcttggaact actggtgttg gtaagactga acttgcacga gctttggctg aagttttatt
     6541 tgacgatgaa catgcaatga tcagaatcga tatgagcgag tatatggaaa agttctctgt
     6601 ttctcgattg ataggcgctc ctcccggtta tgttggatat gaagaaggcg gtcagttgac
     6661 tgaagctgtg cgaagaagac cctactccgt tgttcttctt gatgaaattg aaaaagcgca
     6721 tagcgatgta tttaatgttc tgctacaggt ttta
//
LOCUS       DASWBY010000848         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_370305, whole genome shotgun sequence.
ACCESSION   DASWBY010000848 DASWBY010000000
VERSION     DASWBY010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370305"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="VF870_15340"
     CDS             complement(<1..1328)
                     /locus_tag="VF870_15340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9253619.1"
                     /translation="MKLADVSIRRPVFATMMIMSLIVLGLFSYLKLNVDLYPDVDIPF
                     VVVTTILPGAGPEQIETDVTKIIEDAVNPVEGVDYIQSTSQENVSIVIIAFKLEINGK
                     DAAQDVREKIAAIRANLPTEIEDPVIQRYDPASFPIMSLTVSGNMSDKDITTFTKDVV
                     KKRLENIPGVGAVNLVGGSEKEVQIEVDVARLRAYNISIQDVIMNVGGQNVEIPGGNV
                     IEGKSQLLVRTMGKYNSVEDFNKVIVATPMGKPVYLSDVATVTDGVVEKTSLTRVNGK
                     IAVGLNIIKQSGSNTVQVAHAVNKQLEKIRNEIPEGVAINVALDNSIFIETSIDDVIF
                     DIVYGGLLAVIVIYLFLANFRATVISGLALPASIIASFIFMFALGFTLNMMSLLALSL
                     AVGLLIDDAIVVIENIYRHMSQGETPMEAAKSASEEIGLAVMATTFTIVAV"
     gene            complement(1368..2432)
                     /locus_tag="VF870_15345"
     CDS             complement(1368..2432)
                     /locus_tag="VF870_15345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9253620.1"
                     /translation="MKKLSTVKLDFIMVLLAAVSVFTLQSCWDKPKESEAADKTDYVD
                     TVSVEVQLVALKELSLTKTFSGTLEGEEQANIYAKIPERIMNIKVKVGDYVKAGNVLF
                     ELDKGGASSQFYQAQAAFLNAQKNYERMKNLLAEGAISQQAFDQVETGYEVAKANFDA
                     ARSTVEITSAIAGVVTAINVNVGDLANPQMPLATIANIGRMKAKFNVGENDVPNIYVG
                     QAAQIFSEMNPDVVQSGKIIQISNSANVQSRTFEMQAIFPNTKDRWFKPGMFCRVNVN
                     MKTKKDALVIPLTAIIKQNNDQGVFVINEGKALYKSIKTSITDGNLIEVTSGLKAGEK
                     IVTLGMNNLKDGTIVFISNK"
BASE COUNT          733 a          519 c          402 g          829 t
ORIGIN      
        1 actgcaacga ttgtaaaggt tgttgccatg acagcaagac caatttcttc agaagcagat
       61 tttgcagctt ccatcggcgt ttcaccttgc gacatatgac ggtatatatt ttcaatcacc
      121 actatcgcgt catcaataag caatccaacg gcaagtgata acgccagcaa cgacatcata
      181 ttaagagtga agccgagtgc aaacataaat atgaaacttg cgataataga ggccggtaat
      241 gccaagccgc ttataacagt agctctaaag tttgcaagga aaaggtagat aacaataaca
      301 gctaataaac ctccgtaaac tatatcgaat attacatcat caattgaagt ttcgataaaa
      361 atactattat caagcgcaac attaatagct actccttccg gaatttcgtt ccggattttt
      421 tctaactgct tgttaacggc gtgtgcaacc tgaaccgtat tgctgcccga ttgctttatg
      481 atgtttaatc caactgcaat tttgccgtta actcttgtta agcttgtttt ttcaacaaca
      541 ccgtctgtaa cagtcgcgac atcggaaagg tagacaggtt tgcccattgg tgtagctacg
      601 ataaccttat taaaatcttc tacactatta tatttaccca tcgttcttac aagcagctgg
      661 cttttacctt cgataacatt accaccggga atttcaacgt tctgtccgcc aacattcatt
      721 ataacgtctt gaattgaaat attatatgca cggagtctgg caacatcaac ttcaatttga
      781 acttcctttt cagatcctcc gacaagattt acagcaccaa cgccgggaat attttccaat
      841 cttttcttaa cgacgtcttt ggtgaaagtt gttatatcct tatcagacat attaccggaa
      901 acagttaaag acattatagg aaaacttgcg gggtcatatc tctgaataac ggggtcttcg
      961 atttcagtcg gcagatttgc tctgattgct gcaatttttt ctcttacatc ctgtgctgca
     1021 tctttaccgt ttatttccag tttaaaggcg ataataacaa tagaaacatt ttcctgcgaa
     1081 gtggattgga tgtaatcaac accttcaacg ggattaaccg catcttcaat aatttttgtt
     1141 acatcagttt ctatttgttc aggacccgca ccaggcagta tagttgttac cacaacaaac
     1201 ggaatatcaa catcggggta taagtcaaca ttaagcttta gataagaaaa aagtccaagt
     1261 acaatcaaag acataatcat cattgttgca aacacagggc gtcttattga tacatcagct
     1321 aatttcattt tttattccag aatttcttat tgtaaagttt ttataattta tttgtttgat
     1381 ataaatacga ttgtaccgtc tttaagatta ttcatcccta gtgttacaat tttttctccg
     1441 gcctttaagc ccgacgttac ttcgatcaga ttaccatcgg taattgaagt tttaatcgat
     1501 ttatataatg ctttaccctc attgatcaca aaaacccctt gatcattgtt ttgttttata
     1561 attgcagtaa gcggaattac aagagcgtcc ttttttgttt tcatattaac attaacccgg
     1621 caaaacatac caggtttgaa ccatctgtct tttgtgtttg gaaaaatcgc ctgcatttca
     1681 aatgtgcgtg attgaacatt tgcagaattt gatatctgaa ttattttccc tgattgaaca
     1741 acatccggat tcatttcgga aaatatctga gcggcctggc ccacataaat attaggcaca
     1801 tcattttcac caacgttaaa ctttgctttc attctgccta tgtttgctat ggttgcaaga
     1861 ggcatttgcg gatttgcaag atcaccaacg tttacgttta ttgcagttac tactccagct
     1921 attgccgaag taatttcaac agtacttctt gctgcatcaa aatttgcttt agcaacttca
     1981 tatcctgttt caacctgatc gaaagcctgc tgggatattg ctccttctgc caataagttt
     2041 ttcattcttt cataattctt ctgcgcgtta aggaatgcag cctgtgcctg gtagaactgt
     2101 gatgatgctc cgcctttatc taattcaaat aatacattac cggctttaac ataatcacca
     2161 actttaactt ttatattcat tatgcgctca ggtattttag cataaatatt tgcctgctct
     2221 tcaccttcaa gcgttccgga aaacgttttt gttaaagaca actcttttag tgcaaccaat
     2281 tgaacttcaa ctgaaactgt atccacataa tcagttttat cggctgcttc tgattccttg
     2341 ggcttatccc agcacgattg caaagtaaaa acagagaccg ctgctaaaag aaccattata
     2401 aaatctaatt ttactgttga taattttttc ataaaaataa tactcctatt ggcaattgtt
     2461 aaaaattaat cttcttcctt gtt
//
LOCUS       DASWBY010000849         6420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_370308, whole genome shotgun sequence.
ACCESSION   DASWBY010000849 DASWBY010000000
VERSION     DASWBY010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370308"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2584
                     /locus_tag="VF870_15350"
     CDS             <1..2584
                     /locus_tag="VF870_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX9253621.1"
                     /translation="SENGIASLSDFSERIAQKEHEKISIDHKVDAQVEILEQKKTALK
                     EQSDILLEKFKEITGRENELRNTIAILESKQVNIKKLNDKIQTLTNNIAKTVGFIEEL
                     ANDKDEIQKKIATAESLYLSKQNEKEKLEHELNKLKEKELEQRSLIKSVKDKITFIQN
                     LIDNLEGVSKGAKALLDNKDWAKNGSTILAHIGDSDEKFRFAVEAALKNNLNNVLVES
                     LEDLKRGIEYLNLNDIGKASFYFPHFEEFSNKGLLNKIQSFIEKRKAKKLEQENGFVS
                     WASSSIQTETKWKPFFEKILKNICIVDDLEKAFTLYSKYGSFSFATQNGDFVSRDGVI
                     EAGSVPRLDDSLFGRKQLLENLSTEFPKHELSLIETQKEITNKENLLAAIDLKVLSEQ
                     GRLLVNDLANVDKQIAQFEFEKKKADDEIEKIQHEIKEHAAESNLFDNKKIKLESLLN
                     TEIALRDSEERKKQTLDEEFTEVEKNYNNIRTQRNEINLSIERLVGEKRNTENAIKRA
                     EESIVVISNSISKRKEDIKTAETEIAEIAGQLVSQEQIYNELEETRKTLNTQLEDIEV
                     RHLNLRNDVNDKETNLRSLRKEREVVSDIIHQMDITIKELDIKSANLIDHIKENYSLT
                     LEKKQFDDLQSFDFKQRSEEVLDLKTKVKNLGPINLVAHSEYEEEKERLDFLHKQRDD
                     LVESEKDVVKTIEEINNTAQAQFSETFEKIRGHFVRIFRTLFNPGDEADLKIEENADP
                     LEAKIEIVAKPKGKRPTSIELLSGGEKTLTAIALLFAIYLVKPSPFCILDEIDAPLDD
                     ANIDRFTRILDEFSKDTQFIVVTHNKRTMEAANTLYGVTMQEEGISKLVSVRFNEDLD
                     FVNN"
     gene            2614..4572
                     /locus_tag="VF870_15355"
     CDS             2614..4572
                     /locus_tag="VF870_15355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253622.1"
                     /translation="MQHIKVLLVIFILIIFYYDTAQSQWVNDPSSNTKLVIDPVDPIN
                     ITALKDFSGGAYVFWEDKKGSSESDIYFIHFNKNGDVSFRADGKVVSTRSGVKENPVV
                     FLEPSGNSIVLWKGYDKKKNSDLYIQKLSKNGLRLWTNDGIQLTESKIEKVDYSLRVD
                     SRGYSHTSFITKNSAASNKFSVRYYRITPGGKTLSDSLKGVLYNSNNTISETEIIPDN
                     KGGSYIFWLENQNQKAVLLAQYVDSTGSKKWGSKPLIISKKGNSVLNYSVGKLGKAVY
                     TAITYQGTHKIIYQNLISDQGKLLWGNDGKLLTYQKGSQINPQFAFVDSSVVVSWTNE
                     FEKIKDVFVQRFDVKGNRMWGNNGKRIINIKGNQFGQRIVYDQKNGVVLAWIDKRESN
                     SDANLYIQKIDLGGNFVWDSSGVMISSSKNMEKSYLNLIPDGEGGAVAVFKGSLDKRN
                     DIYGQKIFSTGTYASQILGFTTQVIGDSVKIFWYAANENEGTTYSIYRAGVENPQESE
                     WKLAGSLVKNKKTNTNYYEFYDYPDINGSIYYRIVQNNDKIKPQYSGIEKVDYFRDVE
                     SIVLGQNYPNPFSDSTTISFYLPEEEEVSFEFFNSNIETVKKIEDVEYPAGKNEIVFN
                     AKNLPPGIYFYRLKRGKFVDVKKMIISE"
     gene            4569..5291
                     /locus_tag="VF870_15360"
     CDS             4569..5291
                     /locus_tag="VF870_15360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HEX9253623.1"
                     /translation="MIKRTLKLFVDFDGTITLEDVGEAIFRKFGEDEQVKKIIEDLLS
                     DKISSRKCWDDLCKSVNVINKSELDEFIDSLDIDPTFIPFVKFCNEKGIEMVVLSDGF
                     DYYIERLFNKTRLSGLKYYSNKLFVDDKGILKTEYPYFDNNSPTSANCKKNHIINHSS
                     DDDYTVYIGDGNSDKEAAQYCDFIFAKDGLSRFCSMEKISFYPFRDFAEVQNKLTELM
                     LKKNLRKRHQAQLKRKSAYMAE"
     gene            5294..5890
                     /locus_tag="VF870_15365"
     CDS             5294..5890
                     /locus_tag="VF870_15365"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9253624.1"
                     /translation="MNNIATKIFKYRSYTPIPFVILMLIFENATITSLVVGIIIAITG
                     ELIRVWGVSWAGSETRTTGGVGGTYLVISGPFAYVRNPLYVGNILMYLGLGIMSMALF
                     PYLQIVAILFFIVQYQFIVKEEEGFLITKFGKDYEKYLENVPRFLPRATAYKNESIPQ
                     PNYNLSAGLKSETRTLQAFSIVALLIVIRWYLSLNNIL"
     gene            5890..>6420
                     /locus_tag="VF870_15370"
     CDS             5890..>6420
                     /locus_tag="VF870_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX9253625.1"
                     /translation="MKSKNVLIIAGEVSGDLHGASLIRELKKLDGSLKIFGIGGNKMK
                     AEGMELNYHIDKMAFLGFVEVIKHLPFIKKVQRDILDEVKKRKVREVVLIDYPGFNLS
                     IAKKLKQLNPELKPELIYYITPQVWAWGKGRVNKIKELFNKVLVVFPFEEKFFKSKNV
                     NAEFVGHPLMQEISRYD"
BASE COUNT         2367 a          871 c         1242 g         1940 t
ORIGIN      
        1 ttctgaaaat ggaattgctt ctttatcgga tttttcggaa cgcattgcac agaaagagca
       61 tgaaaaaatt tcaatagatc acaaagttga tgctcaggta gaaattcttg aacagaagaa
      121 aaccgcactc aaagagcaat ccgatattct tctggagaag tttaaagaaa ttaccggcag
      181 agaaaatgaa ttacgaaata caattgcaat tcttgaatca aagcaggtaa atattaaaaa
      241 actgaatgat aaaattcaga cacttacaaa taatattgca aagaccgttg gttttattga
      301 agaacttgca aatgataaag atgaaatcca gaaaaaaatt gctactgcag aatctttgta
      361 tttaagtaag caaaatgaaa aagaaaaact tgaacatgag cttaataaat taaaagaaaa
      421 agaacttgaa cagcgcagtc tgataaaatc tgttaaagat aaaattacct tcatacaaaa
      481 tcttatagat aatcttgagg gcgtatctaa aggggcgaaa gcacttcttg ataataaaga
      541 ttgggctaaa aacggcagta caattcttgc gcacatcggt gattctgatg aaaaatttag
      601 gtttgctgtt gaagctgcac ttaaaaataa tcttaataat gtgcttgtag aatctctaga
      661 agatcttaag agagggattg agtatttaaa tttaaatgat attgggaaag catcttttta
      721 tttccctcat tttgaagaat ttagtaacaa gggcttgtta aataaaattc aaagctttat
      781 tgaaaaacgc aaagcaaaaa aacttgaaca ggaaaatgga tttgtaagct gggctagcag
      841 cagcattcaa acagaaacaa agtggaaacc attctttgaa aaaattctca agaacatttg
      901 tattgttgat gatcttgaaa aagcatttac tctgtattca aagtatggaa gtttcagttt
      961 cgcaactcag aatggagatt ttgtttcaag agatggtgtt attgaggcag gttcagttcc
     1021 gcgtttggat gattctttat tcggaagaaa acaactactt gaaaatctta gtaccgaatt
     1081 tccaaaacat gaattgagtt tgatagagac tcaaaaagaa ataacaaata aagaaaatct
     1141 tcttgcggct attgatctaa aagtattatc tgagcagggc agattgcttg taaatgatct
     1201 tgcaaacgtt gataagcaaa ttgctcagtt tgaatttgaa aagaaaaaag ctgatgacga
     1261 gattgaaaaa attcaacatg agataaaaga acatgcagct gagtcaaatc tttttgacaa
     1321 caaaaaaata aaacttgaat ctttactgaa cacagaaatt gcgcttcgtg attcagaaga
     1381 aagaaaaaaa caaactcttg atgaagaatt tactgaggtt gaaaaaaatt ataataatat
     1441 tcgtactcag agaaatgaaa taaatttaag tatcgaaaga ttggttggtg aaaaacgcaa
     1501 cacagaaaac gcaattaaac gtgcggaaga atcgattgtt gtaatcagca attcaatatc
     1561 aaaacgaaaa gaagatatta aaactgctga aacagagata gcggaaattg ctggacagct
     1621 tgtttcacag gaacagattt ataacgagct ggaagaaaca aggaaaaccc taaacacaca
     1681 attagaagat atagaagtcc ggcatttaaa tcttagaaat gatgttaacg ataaagaaac
     1741 aaatttaaga tcactgcgca aagaaagaga agttgtatcc gatattattc accaaatgga
     1801 tatcaccatt aaagagcttg atataaaaag cgcaaacctt attgatcata taaaagaaaa
     1861 ttattcgctt acacttgaga aaaaacaatt tgatgatttg cagtcattcg attttaaaca
     1921 aagatctgaa gaagtacttg atctgaagac taaagtaaag aacctggggc caataaatct
     1981 cgttgctcac tctgagtatg aagaagaaaa agaaagattg gattttcttc acaagcagag
     2041 agatgatctc gttgaatctg aaaaagacgt tgtaaaaact attgaggaaa ttaacaacac
     2101 tgcacaagca caattttcgg aaacctttga aaaaatcaga gggcattttg taagaatatt
     2161 cagaacacta tttaatcccg gcgatgaagc agacttgaag atagaagaaa atgcggatcc
     2221 tcttgaagca aagattgaga tagttgcaaa acccaagggt aaacgaccaa catctattga
     2281 acttttatcc ggaggagaaa aaacattaac agcaattgca ttgttgttcg cgatttatct
     2341 cgtaaagcca agtccgtttt gtattcttga tgaaattgat gcacctcttg atgacgcaaa
     2401 cattgatcgt tttacacgga tacttgatga atttagtaag gatactcagt ttattgttgt
     2461 aactcataac aagcgcacaa tggaagccgc taatacgctt tacggagtaa caatgcagga
     2521 agaaggaata tccaaattag taagcgtcag atttaatgaa gacctggatt ttgtaaataa
     2581 ttaatgttat ttgaatttat attgaagaaa acgttgcaac atataaaagt attgttagtc
     2641 atttttatac taataatatt ttattatgat actgcccaat ctcaatgggt taacgatcct
     2701 tcttcaaaca ctaagttagt tattgatccg gttgacccga ttaacataac tgcattaaag
     2761 gatttttcag gcggcgctta tgttttttgg gaagataaaa agggaagttc tgaaagtgat
     2821 atttatttta ttcactttaa taaaaacggt gatgtaagtt ttagagcaga tggtaaagtg
     2881 gtttctacaa gaagcggtgt aaaagaaaat ccagttgtat ttcttgaacc atccggaaac
     2941 tcaattgttc tttggaaagg atatgataaa aagaaaaatt ccgacttata tattcagaag
     3001 ttaagtaaaa atggattaag gctttggacg aatgacggaa tccaactgac tgaaagcaag
     3061 attgagaaag ttgattactc tttgagagtc gattcaagag gttattcaca tacaagcttt
     3121 atcacaaaaa actcggctgc ttcaaataaa ttttcagtta gatattatag aataacgcca
     3181 ggtggaaaaa cattaagtga ttctttaaaa ggtgttcttt ataattctaa taacactata
     3241 agcgagaccg agattatacc tgataataaa ggcggctcat atattttttg gttagagaat
     3301 caaaatcaaa aagctgtgct tctggcacaa tatgttgatt caacaggttc aaaaaagtgg
     3361 ggttctaaac cattaataat ttcaaagaag ggtaatagcg ttttaaatta ttcagttggt
     3421 aagcttggca aggctgttta cacagctatt acttaccagg gaacgcataa aattatttat
     3481 cagaatttaa tatctgatca gggaaaattg ctttggggta atgacggcaa actgctcact
     3541 tatcaaaagg gcagtcagat aaatccgcaa ttcgcatttg ttgattccag cgtagtagtt
     3601 agctggacga atgagtttga aaaaattaag gatgtttttg tccaaaggtt tgatgttaag
     3661 ggaaatcgta tgtggggtaa caatggcaag agaattatta acatcaaagg aaaccagttt
     3721 gggcagcgta ttgtttatga tcagaagaat ggagttgtgc ttgcatggat tgataagcgt
     3781 gaaagcaatt ctgatgcaaa cttatacatc caaaaaattg atcttggtgg taattttgtt
     3841 tgggattctt caggcgtaat gatttcatct tcaaagaata tggaaaaaag ttatcttaat
     3901 ttaattcctg atggtgaagg cggagccgtt gctgtattta aaggcagttt agataaacgc
     3961 aatgatattt atggccaaaa aatttttagc acgggtactt atgcctctca aatacttggt
     4021 tttaccacgc aggttatagg agattcagta aaaatatttt ggtatgctgc aaatgaaaat
     4081 gagggaacta catatagtat ttatagagca ggagttgaaa atccgcaaga gagcgagtgg
     4141 aaacttgcag gctcattggt gaagaataag aaaactaata ctaattatta cgagttttat
     4201 gattaccctg acataaatgg atcaatttat taccgcattg tgcagaataa tgataaaatt
     4261 aaaccacaat attcgggtat agaaaaagtg gattactttc gtgatgtaga atcaattgta
     4321 ttgggccaaa attatcccaa tcctttttca gattctacca caatcagttt ttatcttcca
     4381 gaagaagaag aagtatcatt tgaattcttt aacagcaata ttgaaactgt aaagaagatt
     4441 gaagatgttg aatatcccgc aggcaaaaat gagattgtat ttaatgctaa gaatttacct
     4501 ccgggaattt atttctatcg attaaaacgg ggtaaattcg ttgatgttaa aaaaatgatt
     4561 atatctgaat gattaagcgt actctaaaat tattcgttga ttttgatgga acgattaccc
     4621 ttgaagatgt tggtgaagct atttttagga aatttggtga agatgaacag gttaagaaaa
     4681 taatcgaaga tctgttaagt gataagattt cttcacgcaa atgctgggat gatctttgta
     4741 agtctgtaaa tgttattaat aaatcagagc ttgatgagtt tatagattcg cttgatattg
     4801 atccaacctt tattccgttt gtaaaattct gtaatgaaaa aggaatagag atggtagttc
     4861 ttagcgatgg atttgattat tacatcgaaa gactttttaa caaaacccgt ttatccggat
     4921 taaaatatta ttcgaataaa ctttttgtgg atgataaagg aatacttaaa acagagtatc
     4981 cttattttga taataattct ccgacttcag caaactgtaa gaagaatcat ataatcaatc
     5041 acagtagtga tgatgattat acagtttata taggcgatgg aaattctgat aaagaagctg
     5101 cacaatattg tgactttata tttgcaaaag atggattatc aagattttgt tcgatggaaa
     5161 agataagttt ttatcccttt agagattttg cagaagttca aaacaaacta acagagttga
     5221 tgcttaagaa aaatttaaga aaaagacatc aagcacaatt aaaaagaaaa tcagcctata
     5281 tggcggagta ataatgaaca acatcgcaac caaaatattt aagtacagaa gttatactcc
     5341 aatacctttt gttattctta tgctgatttt tgaaaatgca accatcacaa gtttagtggt
     5401 tggaattata attgcaatta caggcgaact aatcagagtt tggggcgtta gctgggctgg
     5461 cagtgaaaca cgcacaaccg ggggtgttgg cggcacttat cttgttatca gcgggccatt
     5521 tgcgtacgtt cgtaatccgc tttacgttgg aaatatttta atgtacctgg gacttggaat
     5581 tatgtctatg gctttgtttc catatttaca gattgttgcg atattatttt ttatagtgca
     5641 gtatcagttt atcgttaaag aggaagaagg atttcttata accaaatttg gtaaagatta
     5701 cgaaaaatat ttagaaaatg taccaaggtt tttacccagg gcgactgcct ataaaaatga
     5761 atcaattcca caaccaaatt ataatcttag tgctggattg aaatctgaaa ccagaacgct
     5821 tcaggctttt agtattgtgg ccctgctaat tgttattaga tggtatttaa gcttgaataa
     5881 tattttgtga tgaagagcaa aaatgtttta attattgcag gtgaagtttc tggtgattta
     5941 cacggtgctt ctttaatacg cgagttaaaa aagttagatg gttctttgaa aatttttggt
     6001 attggcggca ataagatgaa agccgaagga atggaattaa attatcatat agataaaatg
     6061 gcgtttcttg gatttgttga agtgataaaa catttacctt ttattaaaaa agttcagcgc
     6121 gatattcttg atgaagtaaa gaaaagaaaa gtaagagaag ttgtgttaat tgattatcct
     6181 ggttttaatc tgagcatcgc aaaaaaatta aaacaactta atcctgaact taaaccagaa
     6241 ctcatctatt atattactcc gcaagtatgg gcctggggaa aaggcagagt aaataagatc
     6301 aaagaactat ttaataaagt tttagttgtt tttccatttg aagagaagtt ctttaaatca
     6361 aaaaatgtta atgcggaatt tgttggtcat ccgttaatgc aggaaataag cagatatgat
//
LOCUS       DASWBY010000850         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_261935, whole genome shotgun sequence.
ACCESSION   DASWBY010000850 DASWBY010000000
VERSION     DASWBY010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261935"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..1429)
                     /locus_tag="VF870_15375"
     CDS             complement(299..1429)
                     /locus_tag="VF870_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX9253626.1"
                     /translation="MKQTRKDFFKTSGIIAAGSIAASSPLSTFASNIIKTKGSKMKFT
                     FRPYTLELKHVFTVAVNSRTTTPVMLTEIEYEGIKGYGEASMPPYLGESQETANAFLS
                     KVNLEQFTDPFEMENILDYVDSIAEKNTAAKASVDIALHDLVGKLMNQPWYKIWGYDK
                     TKTPFTTFTIGIDTPDVVRQKVKEAEEFKVLKVKLGRDNDKEMIDTIRSVTKVPLTVD
                     VNQGWKDKHFALDMINWLNERNIEFIEQPMPKEQVDDIAWLTQNSPLPIIGDESVQRI
                     PDVIKAHGIYSGINIKLMKCTGMREANKMLNLARSLNMKVMIGCMTETSCAISAASQL
                     SPMMDWADLDGALLIKNDPYEGMQVIDGKVTLTDYPGIGLKS"
     gene            complement(1497..>2284)
                     /locus_tag="VF870_15380"
     CDS             complement(1497..>2284)
                     /locus_tag="VF870_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX9253627.1"
                     /translation="LLRNMIEIYEEDNIAYLEDRVDELINYFQTQYPGKKDIPHVQKL
                     KGMMREWEEELVWAFFGFNTSSVSHMRLGFYQGDIFTENPEMDRDVIPIFNLLKKIKP
                     TIVTVALDPEGSGPDTHYKVLQATAEALKLYEKETGINTIKVWGYRNVWYRFHPAEAN
                     IFIPVSLNSMAIMENTFLNSYGSQRAASFPSWEYDGPFSRLAQRIQVEQYQTVRLCLG
                     KDYFLTHPHPRVQAAYGMVYLKELSQDEFYAHSSELRKLTENY"
BASE COUNT          680 a          453 c          375 g          776 t
ORIGIN      
        1 aagcagccca tttggcagaa ttaacttttg attgttatct cgacggaggg ggaaatctgt
       61 taataaaatg gtttagattt ttcactgact aaagtcggat tcgaaatgac ttttattctg
      121 aaatttcaaa cagagtgatg attcatttat catttgcagt ttaagatttt tcatcgaaaa
      181 tttctcttcg gaaggaatgc ccttggcaga aatgactttt cattgtcatt ttgacaaaag
      241 ggcgaaatct ggactaatac ataagattcc cgtattcgcg ggaatgacac acagtagatt
      301 aactctttaa cccaatacca gggtaatctg ttaaagtaac ctttccatca attacttgca
      361 taccttcata tggatcattc ttaataagta aggcaccatc aaggtcagcc caatccatca
      421 tcggagaaag ttgtgatgca gcagatatcg cgcagcttgt ttccgtcata catccaatca
      481 ttactttcat attaagtgaa cgggcaagat ttaacatctt atttgcttcg cgcattcctg
      541 tacacttcat caatttaatg ttaatgccgg aataaattcc gtgtgcttta attacatccg
      601 gaattctttg cacagactca tcaccaatta ttggaagagg agagttttgt gttaaccatg
      661 caatatcatc tacttgttct ttaggcattg gctgttcaat aaattctata tttcgttcat
      721 tcagccaatt gatcatatca agagcaaagt gtttatcctt ccatccctga tttacgtcta
      781 cagtaagagg aacttttgta actgaccgga ttgtatcaat catttcctta tcattatctc
      841 ttccaagctt tacttttaat actttaaact cttcagcttc tttaactttt tgtctcacaa
      901 catcaggagt atcaattcca attgtaaaag ttgtaaaagg agtttttgtt ttatcgtagc
      961 cccatatctt gtaccagggc tgattcatta atttcccgac aagatcatgc aacgcaatat
     1021 caactgaagc ttttgccgca gtgtttttct ctgctataga atccacataa tctaaaatat
     1081 tttccatctc aaaaggatca gtgaactgtt caaggtttac cttagataaa aatgcgttgg
     1141 cagtttcctg agattcgcca aggtagggag gcattgaagc ttcgccataa cctttaattc
     1201 cttcgtactc aatttcggta agcattactg gagtcgtggt tctggaatta accgcaaccg
     1261 taaaaacatg ttttaactca agtgtataag gtctaaaagt aaatttcatc tttgagcctt
     1321 tggttttaat aatattggat gcaaaagttg atagaggtga tgaagcagca atcgatcctg
     1381 ctgcaataat tccacttgtc ttaaaaaagt cttttcttgt ttgtttcata tttatcctat
     1441 taaatagcga gggacaggac taattctgtc ctacaaaaat tactgcattt tatttactaa
     1501 taattttctg ttaacttcct taattcggat gaatgagcat aaaactcatc ctgagataat
     1561 tctttaagat aaaccattcc atacgccgcc tgcactcttg gatgaggatg cgttaagaaa
     1621 taatccttac ccaaacataa tctaactgtt tggtactgct caacctgaat gcgctgtgca
     1681 agccgtgaaa atggcccatc atattcccag cttggaaaac ttgcagcacg ctgagaaccg
     1741 taactgttca agaaagtatt ttccatgatt gccatagagt ttaaactaac gggaataaaa
     1801 atatttgctt ctgccggatg gaatcgatac caaacatttc ggtatcccca tacttttata
     1861 gtatttatcc ctgtttcttt ttcataaagt tttagcgctt cagctgttgc ctgcagtact
     1921 ttataatgtg tgtccggtcc gcttccttca ggatccaaag caactgtaac aattgttggt
     1981 tttattttct tcaataaatt aaaaatcgga attacatctc tatccatttc agggttttct
     2041 gtaaagatat ctccctgata aaaaccaaga cgcatatgcg aaacagaact tgtattaaat
     2101 ccaaagaaag cccaaacaag ctcttcttcc cattcacgca tcataccttt taatttttgc
     2161 acgtgtggaa tatctttttt acctggatac tgagtttgaa aataatttat taattcatct
     2221 actctatctt caaggtaagc gatattgtct tcttcataaa tctcgatcat gttcctaagt
     2281 agtc
//
LOCUS       DASWBY010000851         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_670731, whole genome shotgun sequence.
ACCESSION   DASWBY010000851 DASWBY010000000
VERSION     DASWBY010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670731"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VF870_15385"
     CDS             <1..314
                     /locus_tag="VF870_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253628.1"
                     /translation="RQALSLEPENPVRLNTLAHFLIDKDLNQNEGLELVDKTLLLSPK
                     NYDYLDTKGLSLYKQGKYKEALDILQKSWDLRMKNAVYNHVAFLHLEAAKKAVAGMRN
                     N"
     gene            complement(606..679)
                     /locus_tag="VF870_15390"
     tRNA            complement(606..679)
                     /locus_tag="VF870_15390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(643..645),aa:Arg,seq:acg)
     gene            919..>1513
                     /locus_tag="VF870_15395"
     CDS             919..>1513
                     /locus_tag="VF870_15395"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX9253629.1"
                     /translation="MKKTARIILTSLLGLILLILVMIITVPVLFKNKIKTKVEQVINE
                     SVNARVKFDDFKLGFFRNFPNLSFSLDNLSVVGIDKFGNDTLAGFKSLDLVFNLTSLF
                     KKSGYEIKSVKIDKAVVNAIYLEDGSANWDIVKETTEVPVEEEAAAPEMKILLKKVSV
                     TNSSVAYIDKGSELEAYLKDLNFDLKGDMTLSQTELQI"
BASE COUNT          504 a          247 c          285 g          477 t
ORIGIN      
        1 ttcgacaagc gctctcatta gaacctgaaa atcctgtaag attaaatact cttgctcatt
       61 ttttaattga taaagatctg aaccaaaacg aggggctgga gcttgttgat aagacgctat
      121 tattaagtcc taaaaattat gattatttgg atactaaagg tttgagtctc tacaagcaag
      181 ggaaatataa ggaagcattg gatattctac aaaaaagttg ggatttaaga atgaaaaatg
      241 ccgtctataa ccatgttgca ttccttcatc tcgaagcagc aaaaaaggct gttgcaggga
      301 tgaggaataa ttaagttcag tcaaataagt catttactct taattgaaac tatacttatc
      361 cacagaaaac agctccggga attttatcca aaaataatta ccttagttta aaaggatcgg
      421 attactgatc ccttagcacg aggtgacgcc ctggcagttt tgttggggca tttttattta
      481 gaatcagttt aactaactgt cattttttaa tgagttttgt aatatgctgt agatttgttg
      541 taggaatagc tatgaataaa agaacgcaat caactgatgt tatgtttatt gcgtccttat
      601 atatgtgatc ccgaagggac tcaaaccccc aaccttctga tccgtagtca gatgctctat
      661 tcagttaagc tacgggacca ttattctgcg atcgcaaata taatgaattt ggagttactc
      721 tggtacagct ggcggataaa gtttggagtg actaaagtgt ggagtactta gagttgaata
      781 ataacaactc taggcactcc aggcactcca aaatcattcc atttttattt ccattgttaa
      841 ttaactactt ttaaatgtaa tattcatgaa aaatttcggt taatatatat ttgatattca
      901 aataaaacca atataaatat gaaaaaaact gccaggataa tattaacatc tttactggga
      961 ttgatattgc tcatcctggt aatgattatt acagttcctg ttctctttaa aaataaaata
     1021 aagacaaaag ttgaacaggt cattaacgaa tcagtgaatg ccagagtgaa atttgatgat
     1081 tttaaactcg ggtttttcag gaatttccca aacctgtcat tttctcttga taatctttca
     1141 gtagttggta ttgataaatt cgggaatgac acacttgcag gctttaaatc gttagatctg
     1201 gtttttaacc ttacaagcct gtttaagaaa tcaggctacg aaataaagtc agttaaaatt
     1261 gataaggccg ttgttaacgc tatttatctt gaggacggtt cggcaaactg ggatattgtg
     1321 aaagaaacaa ctgaagttcc tgttgaagag gaggcagctg caccagaaat gaagatactt
     1381 ctgaaaaaag taagtgttac caacagttct gttgcctata ttgacaaagg ttctgaactc
     1441 gaagcttacc tgaaagacct caatttcgac cttaaaggtg atatgactct cagccagact
     1501 gagctgcaga tat
//
LOCUS       DASWBY010000852         4420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_110868, whole genome shotgun sequence.
ACCESSION   DASWBY010000852 DASWBY010000000
VERSION     DASWBY010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110868"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..2781
                     /gene="uvrA"
                     /locus_tag="VF870_15400"
     CDS             1..2781
                     /gene="uvrA"
                     /locus_tag="VF870_15400"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9253630.1"
                     /translation="MTKEKQQKEKKIIVKGAREHNLKNLSFEIPRNKLVVFTGVSGSG
                     KSSLVFDTIYAEGQRRYVESLSSYARQFLERMNKPDVDLIQGISPAVAIEQKTGSRNS
                     RSTVGTTTEVYDYLRLLFARIGKTICFQCGKVVTKATTGTVADWLEEQKDGERFYLTF
                     PLHDHEGHSVKEEIELLKKRGFFRIYFNKKFYDLNEDEVKLPKNKKDVRVVVDRFKIT
                     KGEIREKLSDSIEVTFKEGENRIVLINADTNDQLEFNKFYECCGVRYEEPEPRFFSFN
                     NPFGACPVCQGFSKVVGVDMNLVIPNPNLTIADGAIAPFHGAKYSTYLRDLIQSARDY
                     KFPIHVPYKQLTEEQISLVKKGFGKYKGLDHFFSELERKTYKIHVRVMLSRYRGYTIC
                     PACKGSRLRREALQVKIDNKSIHEIVKLPIEHSLKFFEELKLSEYDYSIAERVLKEII
                     KRLTFLNNVGIGYLTLDRYSNTLSGGETQRINLATSLGSALVGTLYVLDEPSIGLHPR
                     DNAKLIKILKNLRDIGNSVLVVEHDADMMKEADLIFDMGPKAGVEGGEIVAIGSYSEI
                     IKNKNSLTGKYLSGEFSIPIPDKRNEKKNKSVKIIGAKENNLKNLTLEVPLNKFVVVT
                     GVSGSGKSTLIHDIFYAGLAKYLGNAPSFIGKYDDIKGGEYIDDVVIVDQSPIGKSPR
                     SNPISYIKAFELIRDLFSNTHQARARGYKPGFFSFNVPGGRCETCQGDGYIKVEMQFL
                     ADLYLECDDCNGTRYKKEIREITYRGKNLVDVLDMTVDESLLFFDGNDRIIKLLQVLA
                     DVGLGYIKLGQPSNTLSGGEAQRIKLASYLTSQRDRRHILFIFDEPTTGLHFHDISKL
                     LKCFQLLLERNNSVVIIEHNLDVIKCADYIIDLGPEAGEKGGEVVATGTPEEIVENGN
                     SWTGKYLKEYFN"
     gene            complement(2858..>4420)
                     /locus_tag="VF870_15405"
     CDS             complement(2858..>4420)
                     /locus_tag="VF870_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX9253631.1"
                     /translation="GLITAIIAGFIISFLGGSRVQIGGPTGAFIVIVYGIVQQYGMNG
                     LIIATIMAGVILVVMGLARFGSVIKFIPHPVVVGFTSGIALLIFSTQIKDFFGLAIDN
                     VPSEFFDKWIEYAVSFGSINYYSFGIAALSLFTIMIFPKITHKIPGSIVALIITTILV
                     ELFHLPVETIGSKFGELPSALPLPVFPQIDFATIKNLIGPATTIAILAAIESLLSAVV
                     ADGMIGGKHRSNMELVAQGVANIVTPLFGGIPATGAIARTATNIKNGGRTPVAGIVHT
                     LVLLLITLFFGQYAKLIPMATLAALLIVVAYNMSEWRSFLEIFKSPKSDLIVLLTTFG
                     LTVIFDLTIAIQIGMVLAVFLFMRNMAMVTNVGIITKELKDDDDESFDANAIQNKKVP
                     DGVEVFEINGPFFFGAVSKFRDALNIIENPPKVLIIRMRNVPAIDGTGIHTLEEVYRS
                     TIKHGCQVVLSGVHTQPLMALEQSGFLKIIGEKNVLGNIDDSLDRAREILGLEKLGRP
                     NDFEPTVRRDKK"
BASE COUNT         1505 a          733 c          828 g         1354 t
ORIGIN      
        1 atgactaaag aaaaacagca aaaagaaaaa aagataattg ttaaaggtgc gcgtgaacat
       61 aatcttaaga acttaagttt tgagattcca cgaaataaac ttgttgtttt tacaggtgta
      121 agcggcagtg gaaaatcatc attggttttt gataccattt atgctgaagg acaacggaga
      181 tatgttgaaa gtctttcttc ctacgcaaga caatttcttg agcgaatgaa taaaccagat
      241 gttgatttaa ttcagggaat ctctcctgcc gttgcaatcg aacaaaaaac gggttcacgt
      301 aattcccgtt caacagttgg aaccactacc gaagtttatg attatcttcg tttactcttt
      361 gctagaattg gtaaaacaat atgttttcaa tgcggtaagg ttgttactaa agcaacaact
      421 ggaacggtag ctgattggct tgaagaacaa aaagatgggg aaagattcta tctcactttt
      481 ccgcttcacg atcatgaagg acattctgta aaagaagaaa ttgagttgtt aaaaaaacgc
      541 ggatttttta gaatttattt taataagaaa ttttatgacc tgaacgaaga tgaagtaaag
      601 ttaccaaaga acaaaaaaga tgttagagta gttgttgata gatttaaaat tacaaaaggt
      661 gaaatacgcg aaaaactttc tgattcaatt gaagtaacat ttaaagaagg cgaaaataga
      721 attgttctga tcaatgctga cacgaatgat cagcttgagt ttaacaaatt ttatgaatgc
      781 tgtggagttc gttacgaaga accagaacca agattttttt cattcaacaa cccatttgga
      841 gcttgccccg tttgtcaggg attcagtaaa gtggtcggtg tggatatgaa tcttgtaatt
      901 cccaatccaa atcttacaat tgctgatgga gcaattgcac catttcacgg tgctaaatac
      961 agtacatatc ttcgcgatct tatccaaagt gcacgagatt ataaattccc gatccatgta
     1021 ccttataaac aattaacgga agaacaaatt tcccttgtta aaaaaggatt tggaaagtat
     1081 aaaggacttg atcacttttt ttcagaactt gaaagaaaaa catataagat tcatgttagg
     1141 gtaatgctta gccgttatcg gggatataca atttgtcctg cttgtaaagg ctctcgctta
     1201 agaagggaag cactgcaggt aaagattgat aacaaatcta ttcacgaaat tgtaaaactt
     1261 cccattgaac attcattaaa gttttttgaa gaacttaagt tatcagagta tgattatagt
     1321 attgcagaaa gagttcttaa agaaattatt aagcgattaa cttttctaaa caatgttgga
     1381 ataggttatc tgacattaga tcgttacagt aacacactat ccggcggcga aacgcagaga
     1441 ataaatcttg caacttctct cggttctgct ttggttggga cattgtatgt tttggatgaa
     1501 ccaagtattg gtttgcatcc gcgggataat gcaaagctta taaaaatatt aaaaaatctt
     1561 agagatattg gcaactcggt tttggttgtt gagcatgatg ctgatatgat gaaggaagca
     1621 gatcttattt ttgatatggg accaaaagca ggagttgaag gcggcgaaat tgttgcgatt
     1681 ggcagttatt cagaaataat taaaaataaa aattctttaa caggtaagta tctttccggt
     1741 gagtttagta ttcctattcc tgataaacga aacgaaaaga aaaataaatc agttaaaata
     1801 ataggagcca aagagaacaa tcttaaaaat cttacccttg aagttccatt aaataagttt
     1861 gttgtggtta ctggtgtaag cggttctggt aaaagtactc ttattcacga tattttttat
     1921 gcgggacttg caaagtatct tggcaatgct ccatcattta ttggcaagta tgatgacata
     1981 aaaggcggtg aatatattga tgatgttgtt atagttgacc aatcgccaat tggtaaatct
     2041 ccgcgttcaa atcccattag ctacatcaaa gcatttgaac tgattcgtga tttattttcg
     2101 aacacacacc aggcgagagc acgtggatat aaaccgggat ttttctcatt taatgtgccc
     2161 ggtggaagat gcgaaacctg ccagggtgat ggttacataa aagttgaaat gcagtttctt
     2221 gctgatcttt atcttgaatg cgatgattgt aatggtacac ggtataaaaa agaaattcgg
     2281 gaaattactt accgagggaa aaatctggtt gatgtgcttg atatgactgt tgatgaatca
     2341 ttattatttt ttgatggaaa tgatagaatt ataaaactat tgcaagtgct tgcagatgtt
     2401 ggtttagggt atattaaact tggtcagcct tcaaatacat tgtccggtgg agaagcacaa
     2461 cgaataaaac ttgcttctta tttaacttct caaagagaca gaagacatat actttttatt
     2521 tttgatgaac caacgaccgg attgcatttt catgatattt caaaattgtt aaagtgtttt
     2581 caacttctgc tggaaagaaa taattctgtt gtgattatcg aacataattt agatgtaatt
     2641 aaatgtgcag attacattat tgatcttggt ccagaagcag gtgaaaaagg cggtgaagtt
     2701 gtcgcaaccg gaacaccgga agaaattgtt gaaaatggaa attcatggac aggaaaatat
     2761 ttaaaagaat attttaatta agagttaggc cctaaaaatt taactgaggt catatcaaat
     2821 ttacgattaa ccctccgaac gaatagaaga gttgatgtta ttttttatct cttctaactg
     2881 tgggttcaaa atcatttggt cttcctaatt tttccagacc aagtatttct cttgcccgat
     2941 caagtgaatc atcaatattt ccaagtacgt ttttttcacc aataatcttt aaaaagcccg
     3001 attgttcgag tgccatcaaa ggctgtgtat ggacaccgga taaaactacc tggcagccat
     3061 gctttatagt cgaacgataa acttcttcta aagtatgaat tcccgtccca tcaattgcag
     3121 gtacattacg cattcttatt attaatactt tcggagggtt ttctataata ttcaaagcat
     3181 ccctaaattt agaaactgca ccaaaaaaga acggtccgtt tatttcaaat acttctactc
     3241 catcaggtac ttttttgttt tgtattgcat ttgcatcaaa agattcatca tcatcatcct
     3301 taagctcttt tgtaatgatg ccgacatttg taaccattgc catattacgc ataaacaaga
     3361 acactgcaag caccatcccg atttgaattg ctatagtcag gtcgaaaatt actgttaatc
     3421 caaaagttgt taacaaaaca atcaaatcac ttttaggact tttaaatatt tccaggaatg
     3481 atctccattc actcatatta taagcaacta caataagtaa agcagcaagt gtagccattg
     3541 gaattaattt tgcatactgc ccgaagaaaa gtgttatcaa tagaagaaca agagtatgca
     3601 caatgcccgc aacgggagtt ctgccgccat ttttaatatt tgtagcagtt cgtgcaattg
     3661 cgcctgttgc cggaattcca ccaaataaag gagttacgat gttggcaaca ccttgagcaa
     3721 ctaactccat atttgatcta tgtttgccgc caatcattcc atctgcaaca acggcagaaa
     3781 gtaatgattc aatcgcagcg agtattgcaa tagtagttgc aggtccaatt aaatttttta
     3841 tagtcgcaaa gtctatttga ggaaaaacag gtaatggtaa tgcagaagga agctcaccga
     3901 actttgagcc aatagtttct acgggtaaat gaaaaagctc aactaaaata gttgtaatta
     3961 ttaacgctac aattgatccg ggaattttat gagtgatttt tgggaaaatc attattgtaa
     4021 atagtgaaag cgcagcaatc ccgaatgaat agtaatttat agatccaaaa ctaactgcat
     4081 actcaatcca tttatcaaaa aactctgatg gaacattatc aattgctaat ccaaaaaaat
     4141 cttttatctg agtagaaaat attagtaaag caattccgct tgtaaatcca accacaacag
     4201 ggtggggaat aaattttatt accgatccga atcgtgccaa acccataact actaaaatta
     4261 ctccagccat aattgtagca atgataagtc cattcattcc atattgctga acaattccgt
     4321 aaacaataac aataaatgcg ccggtaggtc cgccaatttg tacacgactg ccgccgagaa
     4381 atgaaattat aaatccagct atgattgcgg tgatcaatcc
//
LOCUS       DASWBY010000853         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_223101, whole genome shotgun sequence.
ACCESSION   DASWBY010000853 DASWBY010000000
VERSION     DASWBY010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223101"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VF870_15410"
     CDS             <1..140
                     /locus_tag="VF870_15410"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX9253632.1"
                     /translation="PEPSNEDISITRKIVEAGKMMDIPVFDHLIIAGNTYTSFVERRL
                     I"
     gene            428..703
                     /locus_tag="VF870_15415"
     CDS             428..703
                     /locus_tag="VF870_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253633.1"
                     /translation="MNITKSLRTAVLFGSIALTTAFFSGCGGLSDAQIKELSDLKAKT
                     ASLEAEANSLRDERAKLEAQMEDTNRKLAECEKQKEEARANLEKLPK"
     gene            748..1425
                     /locus_tag="VF870_15420"
     CDS             748..1425
                     /locus_tag="VF870_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9253634.1"
                     /translation="MNLKIKLLGIVSLFLLLTVVNFAQEEMTEEQWESEMTAYKNKQV
                     ALETEIAALKTDIDNLKAMNLQDPEECIDELYQLVGATRNDVNNYRKAVNELDGKIKR
                     KEGPKADRQTDLNALKTNKISALPEFYNKVHNQMQKDLDNWVEAPTEINYTVVKGDCL
                     WNIAKKKEHYGNGFAWPVIYQANRDQIKNPDLIYPKQTFKIPNLTEEEKSKYEKLRVN
                     YKPAPVQ"
     gene            1501..2463
                     /locus_tag="VF870_15425"
     CDS             1501..2463
                     /locus_tag="VF870_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9253635.1"
                     /translation="MTTAEKKITLNGIDMLSLLGVNDANLHILEDRFNSTISVRGDIV
                     NIKGVLEEVELVEKVIKEMAYVLNTSGRLNRNDVDTILNLTVEGKEIINEDEFDSIVL
                     YTKNDVVKAKTPGQKKYLQVANKNDICFAIGPAGTGKTYLAVALAVSALKRGIVKRIV
                     LARPAVEAGESLGFLPGDFQEKIDPYLRPLYDALDDMMPSEKLKGYIEKSIVEIVPLA
                     YMRGRTLNNAFVILDEAQNATDIQMKMFLTRLGANSKAIITGDITQIDLPSKSKSGLI
                     QAKEILSSITGVGFVYFDREDVVRHKLVKDIIDAYDKFNGNGNS"
     gene            complement(2460..>2808)
                     /locus_tag="VF870_15430"
     CDS             complement(2460..>2808)
                     /locus_tag="VF870_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX9253636.1"
                     /translation="DWEKKEDVWKKVIEEIEEMHEIEKIKSQFPSKGTQEELQIKLEK
                     EVGDVFFAMVNYARFLGVNPEDALRLTNAKFIKRFNYVQQKVKETGKAINESNLEEMD
                     KYWNESKKLTGFD"
BASE COUNT          970 a          447 c          504 g          887 t
ORIGIN      
        1 atccagaacc aagtaatgaa gatatatcaa ttacacgaaa aatagtggaa gcaggcaaaa
       61 tgatggacat tcctgtgttt gatcatttaa ttattgccgg gaatacttat acaagttttg
      121 tggagagacg actgatttag atatttatta tcgaaggtta gtttttttcc attctaaaga
      181 tgtagtcctt ttttcatcct ttgggaggat tgaattaaat tgattatatc aacagtttaa
      241 ataagtcgtt aagtgttagt tttagtaggg caggttaatt tgaactgaaa aaagaattaa
      301 atttttagtc aatactttct tttatcaatt tttttcatat atttcaaaga gtgttaatca
      361 aatttttaga caaaaattga atatttcgta ttttttaatc actaaccata atattcggag
      421 gtatcgaatg aacataacaa aatcattgag aactgccgta ttgttcggca gtattgcact
      481 tactactgcc ttctttagcg gatgcggcgg attgagtgat gctcaaataa aggaattgag
      541 cgatcttaaa gctaaaactg catcacttga agcagaagca aattctttga gagatgaacg
      601 agctaaactt gaagctcaga tggaagacac aaacagaaaa cttgctgagt gcgaaaaaca
      661 gaaagaagaa gcaagagcta acctagaaaa actacctaag tagtaaatta attaatgatt
      721 tttaaaaata ttcagaggaa ataaataatg aatcttaaaa taaaattatt aggcatagtt
      781 tccctgtttc tacttttgac tgttgtaaat tttgcacagg aagaaatgac tgaggaacag
      841 tgggaatccg aaatgactgc ttacaagaat aagcaagttg ccttagaaac cgaaatagct
      901 gctttaaaga ccgatatcga caatcttaaa gctatgaatc ttcaggatcc tgaagaatgt
      961 atcgacgaat tatatcagct cgttggtgct acacgtaatg acgttaacaa ctatagaaaa
     1021 gctgttaatg aattagatgg taagatcaaa agaaaagaag gcccaaaagc tgacagacaa
     1081 actgatctta acgctcttaa gacaaataaa atttctgcat tgccagaatt ttataacaaa
     1141 gttcacaatc agatgcaaaa agaccttgat aactgggtag aggctccaac agagattaac
     1201 tacacagtag ttaaaggtga ttgtctttgg aatatcgcaa agaaaaaaga acactatggt
     1261 aatggatttg cttggccggt tatttatcaa gcaaacagag atcagattaa gaatcctgat
     1321 ttaatttatc caaaacaaac tttcaagatt cctaatctaa cagaagaaga aaaatcaaaa
     1381 tatgagaaac taagagtaaa ttataaacca gctcctgttc aataattgat ttcttgttct
     1441 cttaaaatta ttataagccc ttatctttgc tgatgagggc tttttatatt taaatctgat
     1501 atgacaaccg cagaaaaaaa aataacactt aatggcattg atatgctgtc gctgcttgga
     1561 gtgaatgatg caaatcttca tatccttgaa gaccgcttca actctactat tagtgtgcgt
     1621 ggcgatattg taaatattaa aggcgtactt gaggaagttg agcttgttga aaaagtaatc
     1681 aaagaaatgg cttatgtttt gaacacaagc ggcagattaa accgaaatga tgttgataca
     1741 attctaaatc ttacggttga gggaaaggaa ataatcaacg aggatgaatt tgattcgata
     1801 gttctctaca ctaaaaatga tgttgtgaaa gctaaaactc cgggtcaaaa aaaatattta
     1861 caggttgcca ataaaaatga tatctgtttt gctatcgggc ctgctggtac aggtaaaacg
     1921 tatcttgctg tggcgctagc tgtttccgcc cttaaaagag gaattgtaaa acgaatcgtt
     1981 cttgcccgtc ctgcagttga agcaggcgaa agcctcggat ttttacctgg agattttcag
     2041 gagaaaatcg atccctatct tcgtccatta tacgatgctc ttgatgatat gatgccgtct
     2101 gaaaaattaa aaggctacat tgaaaaaagt atcgttgaaa ttgtgccctt agcttatatg
     2161 cgcggtagaa cgttgaataa tgcatttgtc attcttgatg aggcacaaaa tgcaaccgat
     2221 attcaaatga aaatgtttct tactcgactt ggcgcaaact caaaagcaat aataaccggg
     2281 gatattactc aaatcgattt gccatctaaa tcaaaaagcg gattgattca ggctaaagaa
     2341 atattaagct caataactgg tgttggtttc gtttactttg acagagaaga tgtagttaga
     2401 cataaattag ttaaagatat tattgacgct tacgataaat ttaacggtaa cggaaatagt
     2461 tagtcaaatc cggttaattt tttgctttca ttccagtact tatccatctc ttcaagattt
     2521 gattcgttta tagctttacc ggtctcttta actttttgct gtacgtaatt aaatctcttt
     2581 ataaattttg cattcgtaag tctcagtgca tcttctggat ttaccccaag aaatcttgca
     2641 taatttacca ttgcaaaaaa aacatcgcct acttcttttt ctaactttat ctgtaattct
     2701 tcttgagtgc ctttagatgg aaattgtgat ttaatttttt caatctcgtg catttcttca
     2761 atttcttcaa tcactttttt ccaaacgtct tccttctttt cccaatca
//
LOCUS       DASWBY010000854         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_261964, whole genome shotgun sequence.
ACCESSION   DASWBY010000854 DASWBY010000000
VERSION     DASWBY010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261964"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1836
                     /locus_tag="VF870_15435"
     CDS             184..1836
                     /locus_tag="VF870_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX9253637.1"
                     /translation="MSGIDRRKFLRTSALSAAAVVTAPIFSKFGNIIASNKGDGIIRP
                     YPHPWMPKMDFVYLTDHNEDPFKSSIEITQDGIKLPSDIGDKKFGINSRWFIEGFGFV
                     YLTADNGGEHFTLREVQSREKLNLNYEYAKSRIIRNRNVRERYLKSGTLFSREVNHLV
                     NLSEELFEDATKKQNDAERCADYSDRALKYALIVGENIELEHARSEITRQNRKDKVHF
                     GCESRQMVWAQSEGFTKRFPELFNTATVTHYVWDSWYQLFEPSEGNYNWGIKDDIVNF
                     LMEHNITIEGRPLFWFHPTVTPDWLKQKSYSELKDYMEKHVYDLVTHYGDKVLEWEVM
                     NEYHDWANIFNHNPEQITEITRFAFDKTKEVNPNVKRISNNCCLWGEYAARGRMARMD
                     ATRPLRNARKYVEDILNAGVDVDVVGIQIYFSRRDLSDIVRMVERFEKFGKPIYITEI
                     GASSGPTNDTVFNGSRGIPKDPYDWRRPWDEELQADWLEEVYTIYYSRPLIKAINWYD
                     FSDFRPFIVNGGIVREDTSPKMSFDRLKNLLNGWNRLPGQNP"
     gene            1854..>2291
                     /locus_tag="VF870_15440"
     CDS             1854..>2291
                     /locus_tag="VF870_15440"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9253638.1"
                     /translation="MNILIIGGTGFLSSRIAEKLLSRGHNLTLLVRGRTKSKFAGNKN
                     LSFVYGDRNDKKILNELTSNSTFDVVYDMVAYSENDSQTAIDVFNNKIGRFIHCSTIS
                     VYMLAEEVRLPVTEDQINLPLMKEFRRSPFGWEYGINKIKCENV"
BASE COUNT          808 a          334 c          499 g          650 t
ORIGIN      
        1 ccgtgtgaat ggtgggttag tgagagaaga tagttcgcct aaattgtctt tcgacagatt
       61 gaaaaaactg ctcggtagtt ggaacagatt accaaatcag ggtaaataaa aaattataaa
      121 attaatattt tgataagctt aaaagaaaaa tggaattcac tttaattata atcagaggtt
      181 ttaatgagcg gaattgatag aagaaaattt ttgagaacat ctgccttgag cgcagcagcg
      241 gtagtaacag ctcctatatt ttcaaagttt ggaaatatta tagcttcaaa taaaggcgat
      301 gggataattc gaccataccc gcatccatgg atgccaaaaa tggactttgt ttatcttaca
      361 gatcataacg aagatccatt taaatcttct atcgaaataa cgcaggatgg aattaaactg
      421 ccgtcagata taggggataa aaagtttgga atcaactcgc gatggttcat tgaaggattt
      481 ggatttgtat acctgacagc tgataacggt ggtgaacact ttactttaag agaagtccag
      541 tcacgtgaaa aattaaatct gaattatgaa tatgcaaaaa gcaggattat tagaaatcgt
      601 aatgtaagag aaagatattt gaagtctggc acattgtttt cccgggaagt caatcatctc
      661 gtaaatcttt ctgaagaatt atttgaagat gcaactaaaa aacagaatga tgctgaacga
      721 tgcgcggatt attcagacag agcgttgaag tatgccttaa tagttggaga aaatattgaa
      781 ctagaacatg cgcggagtga aataacaaga cagaacagga aagacaaagt tcacttcgga
      841 tgtgaaagca gacaaatggt ctgggctcag tctgaaggat ttacaaaacg ttttcctgag
      901 ttgttcaata cggccactgt tacacactat gtgtgggatt catggtatca gttatttgag
      961 ccatcggagg ggaattataa ctggggaata aaagatgata tcgtgaactt cctgatggaa
     1021 cacaatatta caatagaagg ccgaccccta ttctggttcc acccaacagt aacacctgat
     1081 tggctgaaac aaaaaagtta ttccgaacta aaagattata tggagaaaca tgtttatgat
     1141 cttgtaactc attacggtga taaagtcctg gaatgggaag tgatgaatga atatcatgat
     1201 tgggctaata tatttaatca caatcctgaa cagataacag aaattacgag attcgctttc
     1261 gataaaacaa aagaagtaaa tccaaacgtg aagagaatat ctaataattg ttgtttgtgg
     1321 ggtgaatatg cagcaagagg cagaatggca aggatggatg caacgagacc attaagaaat
     1381 gccagaaagt acgtagaaga tatcctgaac gctggagttg atgtcgatgt tgttggaatt
     1441 caaatttact tttcgcgacg ggacctttca gatatagtta ggatggtaga acgatttgaa
     1501 aaatttggta aaccaattta cataactgaa ataggagcca gttcaggtcc tacaaatgat
     1561 actgtcttta acggttcccg gggaatacct aaagacccgt atgattggag aagaccttgg
     1621 gatgaagagc ttcaagcaga ctggcttgag gaagtgtaca caatttatta tagcagacca
     1681 ttaattaaag caatcaactg gtacgacttt tcagacttcc gtccgttcat cgtgaatgga
     1741 ggtattgtga gagaagatac ttcgccaaaa atgtccttcg acagattaaa aaatttattg
     1801 aatggctgga acagattgcc gggacaaaat ccttaattta ttttgaaatt attatgaaca
     1861 ttctcattat aggcggcacc ggctttttaa gcagcagaat tgcagagaaa cttttatcaa
     1921 ggggacataa tctgactttg ctagtcaggg gtaggacaaa atctaaattt gctggtaata
     1981 aaaatttaag tttcgtttat ggtgatagga atgataaaaa aatattaaat gaactaacat
     2041 caaatagtac atttgatgta gtttatgata tggttgctta ctcggaaaat gattctcaaa
     2101 cagcaataga tgtatttaat aataaaattg gaagatttat tcattgcagc acgatttcgg
     2161 tttatatgct agctgaagaa gtgagattac ctgtaactga ggatcagatt aatcttcctt
     2221 tgatgaagga attccggaga agtccctttg gctgggaata tggtataaat aagattaaat
     2281 gtgaaaatgt g
//
LOCUS       DASWBY010000855         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_561989, whole genome shotgun sequence.
ACCESSION   DASWBY010000855 DASWBY010000000
VERSION     DASWBY010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561989"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VF870_15445"
     CDS             <1..1035
                     /locus_tag="VF870_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX9253639.1"
                     /translation="CGVSVNDNSMMIPIKSVSAVIGVGKNVEKKNYECSICEIDFCYK
                     REKMHKISDIINTGKLLTSDGAWGTYLFKKGLPAGSCPEEWNLTHYDDVLDIAKSYVL
                     AGSDIISTNSFGSNVFKLSQYNLQEKLSEICQTSAEISRNAAGNDKIVMASLGPTGKF
                     LIMGDVTSQELYASFNQQAIAFEKGGADAVCIETFYALDEAEQAIKAVKENTSLEVIC
                     TFTFDKSENGYKTLMGVTPQQMAESLITYGADIIGANCGSGFEDMIEIVKMIRKVAAS
                     LPLIIQANAGLPVFENGELIYSETPEKIKEIIPKLIEAGANIIGGCCGTTPEHIKVIS
                     EIAGKFNQKS"
     gene            complement(1040..2080)
                     /locus_tag="VF870_15450"
     CDS             complement(1040..2080)
                     /locus_tag="VF870_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX9253640.1"
                     /translation="MSKISSPDINSLLKTLKREKVDYFPLAELGVHPKIKELFLAKKI
                     EGLKDEVDFWYKAGYDYIKLQPKADFNPMKIGLDNNVSYNDDGTIFRKWASESNGVIS
                     NFDDFEKYTFPIASDFEYTNFEKVKPLLPEGMGVIGQYGDIFTMTWEMMGFESFSIAL
                     FENEELINSLNERLGNLVLSMFEYFAQSNVVDAIWYSDDIAYTNSLMVSPQVLDKYFF
                     PWLKKIGDLAKQYNKPFIYHTDGILYDVMDRIIDCGVDAIHPIEPKAMNIADIKKRYG
                     NKLCLIGNIDVDLLSRGSTEEIKKNVYKNVEEAGYNGGYCVGSGNSIPEYVKLENYKA
                     MIEAVKEINSKL"
BASE COUNT          767 a          371 c          386 g          777 t
ORIGIN      
        1 tgtggagtat cagtaaatga taattccatg atgataccta taaaatctgt tagtgcagtc
       61 atcggagttg ggaaaaatgt tgaaaagaaa aattatgaat gttctatttg tgaaatagat
      121 ttttgttata agcgggaaaa aatgcataaa atatctgata ttataaatac aggtaaactt
      181 ttaacttctg atggagcctg gggaacatat ctttttaaaa aaggattacc ggctggaagt
      241 tgtcccgaag aatggaatct tacacattat gatgatgttc ttgatattgc aaaaagttac
      301 gttcttgctg gttccgatat aatctcaaca aatagttttg gttccaatgt atttaaactt
      361 tctcaatata atcttcagga aaaactttca gagatatgtc aaacatcagc agagatatct
      421 cgcaatgcag caggtaatga taaaatagta atggcttctc tcggtccaac gggtaagttt
      481 ttaataatgg gtgatgttac aagtcaggaa ttatatgctt catttaatca acaggctatt
      541 gcatttgaga agggcggtgc ggatgcagtt tgtatagaaa ctttttacgc gttggatgaa
      601 gcagaacaag caatcaaagc agttaaagaa aatacttcac tagaagttat ttgcactttt
      661 acttttgata aatctgaaaa tggttacaaa actttgatgg gtgtaacacc acagcaaatg
      721 gcagaatcat taatcactta cggtgctgat atcattggtg caaattgcgg ttcgggtttt
      781 gaagatatga ttgaaattgt aaagatgatt cggaaggtcg ctgcaagcct ccctctaatt
      841 atccaggcaa atgccggatt acctgttttt gaaaatgggg aacttattta ttcagaaact
      901 cctgaaaaga taaaagaaat tattccaaaa ttaattgagg caggtgcaaa tattatcggt
      961 ggatgctgtg gtacaactcc ggaacacatt aaagttattt ctgaaattgc tgggaagttt
     1021 aaccaaaaga gctaaccagt taaagctttg aattaatttc tttaactgcc tctatcattg
     1081 ctttgtaatt ctcaagttta acatattctg gaatcgaatt tccggatcca acacaataac
     1141 ctccattgta tccagcttct tcaacatttt tgtaaacatt tttttttatt tcttcggttg
     1201 agcctctgga aagtaaatca acatcaatat ttccaataag gcagagctta ttcccatatc
     1261 tttttttgat atcagcaata ttcattgctt taggttctat tggatggatg gcatcaacac
     1321 cgcaatcaat aattctgtcc ataacatcat ataaaattcc atctgtgtga tatatgaatg
     1381 gtttgttata ctgctttgca aggtcgccaa tcttttttaa ccaagggaaa aaatatttat
     1441 ccagaacctg tggtgatacc atcaaggaat ttgtgtaagc aatatcatcg ctgtaccaga
     1501 tcgcatccac aacattggat tgagcaaaat attcaaacat acttaaaaca agattaccca
     1561 aacgctcatt aagggaattt ataagttctt cattctcaaa aagtgcgatt gagaaagatt
     1621 caaaccccat catctcccaa gtcattgtaa aaatatcgcc atactgccca attactccca
     1681 ttccttcagg cagcaatggt tttacttttt caaagtttgt gtattcaaaa tcagaagcta
     1741 ttgggaatgt atatttctca aaatcatcaa aatttgaaat tactccattg ctttccgatg
     1801 cccattttct gaagatagtt ccgtcatcgt tataagaaac attattatcc aatccaatct
     1861 tcatcggatt aaaatcagct tttggctgaa gttttatata atcatatccg gctttatacc
     1921 aaaaatcaac ttcatccttt aatccttcaa tctttttagc tagaaatagc tcttttattt
     1981 ttggatgaac accaagttcg gcaagtggaa aataatcaac tttttcccgc ttaagtgttt
     2041 ttaataatga gttaatatca gggctgctta tttttgacat attagattaa attgaattat
     2101 tagtaataaa attaaatata atttatttgt aagttatatt gattttgaga ttaaaattga
     2161 taaatttcac atcaggacaa aacaataata ttgaaagcga tgctatgttt agaatattaa
     2221 tatcctttgt tttaaccgca tttctatttt taggaataat cggtactgct aaatatatgt
     2281 ttagttctgt gaaagcaaat g
//
LOCUS       DASWBY010000856         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_223276, whole genome shotgun sequence.
ACCESSION   DASWBY010000856 DASWBY010000000
VERSION     DASWBY010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223276"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VF870_15455"
     CDS             complement(<1..373)
                     /locus_tag="VF870_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253641.1"
                     /translation="MKKILLIIILISSLISAQNNPMSYQRWNIFDINKVRTEFSNTGL
                     LCNGNEQSSSLAREPSFEYPAGSGFSWGTGVGVMVGAPINQDPGAVGGFPNPQNNYSA
                     FCDATLDEGPAAFWDEEHFYPY"
     gene            complement(394..1437)
                     /locus_tag="VF870_15460"
     CDS             complement(394..1437)
                     /locus_tag="VF870_15460"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9253642.1"
                     /translation="MKYFLINKNVQRIFLIIIFSALTLQTQEINAQQVDYSGTSAANF
                     LKIGVGSRPMAMGDAAIATINSAEALYWNVAALTRIEDEFSFAVSTMEWLVDSRNSYV
                     AASYKADDLGSFGIDLQYLDYGKIEETTVYDQDGTGRFLSASDMVIGIGYARQLTDRF
                     SFGIKIKYINETIADATGNAFAIDLGTVFLTSFFNNKLRLAASLSNFGTKLKFIGDDL
                     SVTYTVPDNPGGKQVPAELTTLEWDIPLLLRFGISNYFIDNEDLSILAAYDIVDSRDY
                     EVRHNLGAEIGLYKMVYLRGGYKFNYDEIKYTVGIGLDFKNIIDYNLKLDYVFLDFGD
                     FGSLNQFSLIFNL"
     gene            complement(1450..>2147)
                     /locus_tag="VF870_15465"
     CDS             complement(1450..>2147)
                     /locus_tag="VF870_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253643.1"
                     /translation="WGSESFTDFQGGTHYIPLAQYDLENGLKGYYQTLPEYAWYYLGD
                     DKWNDLRFVVEGDSINGFDLGNYKLQHYTDGDTVNVFVDRDVLNGVEYKYYVAAYDTG
                     NGIIGPLENSASANPEEFNNTVSVRPELPVAIDNLSEVKVVPNPYIVSTIWESSWEEH
                     TVQFTGLSKQATIKIFNSSADLVKTLYKDNESSIQQWDLKNEYNQQVAPGVYFYYIDS
                     PIGSTSGKFFVIL"
BASE COUNT          658 a          449 c          351 g          689 t
ORIGIN      
        1 ggtaaggata aaaatgttct tcatcccaga atgctgcggg accctcatct aaagttgcat
       61 cacaaaaagc agagtagtta ttttgaggat tcggaaatcc gccgacagct cccggatcct
      121 gattgatcgg agcgccaacc attaccccta ctcccgttcc ccagctgaac ccgcttcctg
      181 ccggatattc gaaagaaggt tctcttgcaa gcgaacttga ctgttcgttc ccgttacaca
      241 gtaagcctgt gttgctgaac tcagttctga ctttattaat atcgaaaata ttccagcgct
      301 gatagctcat cggattattt tgagctgaaa taagtgatga aattaaaatt ataatcaata
      361 gaattttttt cattataaaa cctgacttct tagctataga ttaaatataa gcgagaattg
      421 attcagcgat ccaaaatcac caaagtctag gaagacataa tctaatttca gattgtaatc
      481 tattatgttt ttaaaatcca gtcctattcc aaccgtatac tttatttcat cgtaattaaa
      541 cttgtatccg cctcgtaaat aaaccatctt atatagtcct atttcagctc caagattatg
      601 acgcacttca taatctctgc tgtcgactat atcatatgca gcaagaatgg ataaatcctc
      661 attgtcaata aagtaatttg aaattccgaa tcttaataac aatggtatat cccactcaag
      721 agtggttaat tcagcaggta cctgcttgcc gcccggatta tcaggaacag tgtatgtgac
      781 agataaatcg tctcctataa atttcagctt ggttccgaaa tttgataatg acgccgccag
      841 ccttaattta ttgttgaaga aggaagtcag aaaaaccgta cccagatcaa ttgcaaaagc
      901 attgcctgtt gcgtcggcaa tagtttcatt tatatatttt attttaattc cgaaagaaaa
      961 cctgtctgta agctggcgtg cataacctat tccaattacc atgtcacttg ctgataaaaa
     1021 tcttcctgtc ccgtcctgat cgtagactgt agtctcttca atctttccgt aatcgagata
     1081 ctgaagatct attccaaaac ttcccagatc gtcagcttta tatgaagcag ctacgtatga
     1141 attccttgaa tcaacaagcc attccattgt tgaaacggcg aatgaaaatt catcttcgat
     1201 cctggttaag gctgcaacat tccaataaag cgcttcggca gaattgattg tggcaatagc
     1261 cgcatcaccc atagccattg gtcttgaacc aactcctatt ttcaaaaaat tagcagctga
     1321 ggtacctgaa taatcaacct gctgtgcatt aatttcttgg gtctgtaatg ttaaggcgga
     1381 gaatattata attaaaaata ttctctgaac attcttatta attaaaaaat acttcatagc
     1441 ttattcctgt taaagtatta caaaaaattt acctgatgtt gatcctatag gtgaatcgat
     1501 ataatagaaa tagactcccg gagcaacctg ttgattatat tcgttcttta aatcccactg
     1561 ctgtatgctg ctttcattgt ctttatatag agttttgacc aagtcagcgc ttgaattaaa
     1621 tattttgatg gtagcctgtt ttgataagcc cgtaaattga acagtatgct cttcccagct
     1681 tgattcccat atagttgaaa ctatataagg gtttggtaca actttgactt cgcttagatt
     1741 atcaattgcg acaggaagtt ccgggcgaac tgatactgta ttattaaatt cttccggatt
     1801 agctgaagca gaattttcca atggcccgat aattccgttt cctgtatcat aagcagctac
     1861 gtaatattta tattcaaccc catttaatac atctctatct acaaaaacgt ttacggtatc
     1921 tccgtctgta taatgttgta acttataatt tccaagatca aatccattta tagaatcacc
     1981 ctcaactaca aatcttagat cattccactt atcatctcct aaataatacc atgcatattc
     2041 aggcagtgtt tgatagtagc ccttaagtcc attttccaaa tcatactggg caagtggtat
     2101 ataatgtgtc cctccttgaa aatctgtaaa actttctgaa ccccatg
//
LOCUS       DASWBY010000857         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_187660, whole genome shotgun sequence.
ACCESSION   DASWBY010000857 DASWBY010000000
VERSION     DASWBY010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187660"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VF870_15470"
     CDS             complement(<1..1209)
                     /locus_tag="VF870_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253644.1"
                     /translation="MKNFAFTVLLASLLTAFLHSQSYNTTWIGGFYTPTKAFDVKVNG
                     KYAYVVGWEDDFYGFYGSFLIIDVSFPTVPYLVGSTYTMDAYGIDISGNYAFIAEGNS
                     GLRIYNITDPTNPFLIGQRILPSSAKDIKVVGDLAFVADEYAGLRIIDISNLQDPIEI
                     GFFDSPGRAFDVEVRDNIAYLADTDEGFLCIDISNPSNPILISEYPAEDVVVDIELKD
                     DLAYYTDGYSGIKVLDISNPNNLVLVGSLYMPYWCLGIKVSGNYAYIAAWEFGGLRIV
                     DISDPANMQEVGFYNTWGKSRDIDVVGSLAYIADWDGGGLQIINNNILNPLPVELVSF
                     DADYINGNVNLSWITATELNNSGFDVERKTEDGNWDKIIFIKGNGTTTETNHYFFSDD
                     IKKLNTSQLIY"
     gene            complement(1296..2282)
                     /locus_tag="VF870_15475"
     CDS             complement(1296..2282)
                     /locus_tag="VF870_15475"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9253645.1"
                     /translation="MSTKIKNKKVLITGGLGMIGSTLANKLVVMGAEVTLLDSLIKPY
                     GANIFNVEKISQLIKINISDIRDKESVKVLVQDKDIIINLAGQVSHNDSLQNPYLDTD
                     INYIGHLNVLEAVKNNNPTAKILYSGSRLQFGKIISNPVAEDHILNPLTPYAVNKTAA
                     ENLYLYYNRVFNIPVVLFRIANPYGPRCQMQHSKYAIINWFIRNAMEDKEITIFGDGS
                     QIRDYIYVDDLADAFILAGVDENIRSEIFNIGCGTGTSFRDMVQLVVDIVGKGKIKYQ
                     PWPQDYLNVETGDYITNISKITKMLNWVPKTDLKKGIEQTVAYYQEFGKHYW"
     gene            2356..>2917
                     /locus_tag="VF870_15480"
     CDS             2356..>2917
                     /locus_tag="VF870_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX9253646.1"
                     /translation="MISSKQRYNFCTIFDYSYFAKGLALYYSLTKVCDCHLYVFTPDE
                     RCKKALQEKQFQNLTIVDFHEVENKELLEVKSKRDVAEYFWTIKGSCLQFLFSKFNLE
                     IATYIDADTFFYSSPEPFFKEMENKSVLILPHNFSLKYQHEIVNGVYNAGYISFRNDK
                     DGLSALKWWTDKSIEWCFKKKEKGKFG"
BASE COUNT          993 a          527 c          434 g          963 t
ORIGIN      
        1 atatatcaat tgggatgtgt ttaacttttt aatatcatct gaaaagaagt aatgattagt
       61 ttctgttgta gttccatttc ccttaatgaa aataatttta tcccagttac catcttcagt
      121 ttttctttca acatcaaaac cagaattgtt caactcagtt gctgtaatcc acgaaagatt
      181 aacattgccg ttaatataat cagcatcaaa tgaaactagt tctacaggaa gaggattgag
      241 aatattattg ttaattattt gtaaacctcc gccatcccaa tcggctatgt aagctaatga
      301 gccaacaaca tcaatatcac gtgattttcc ccaggtatta taaaatccga cttcttgcat
      361 atttgcggga tcagaaatat ctacaattct taatccacca aattcccaag ctgcaatgta
      421 agcgtaattt ccagaaactt taattcctaa acaccagtat ggcatatata aagaaccaac
      481 caacactaaa ttatttggat tagaaatatc caaaactttt ataccggaat aaccatctgt
      541 gtaatatgct aaatcatctt ttagttcgat atctacaaca acatcttctg cgggatattc
      601 actaataaga atgggatttg aaggattact tatatcgata cataaaaatc cttcgtcggt
      661 gtcagctaaa tatgctatat tatctctcac ttcaacatca aatgcccttc ctggtgaatc
      721 aaaaaaaccg atttctatgg gatcctggag atttgaaatg tcaattattc ttaatccagc
      781 atactcatca gcgacaaagg ctaaatcgcc aactacttta atatcttttg cactacttgg
      841 taatattctc tgcccaatta aaaaaggatt tgtaggatct gttatattgt aaatccttaa
      901 tccggaatta ccctccgcaa tgaatgcgta attgccacta atatctattc catatgcatc
      961 cattgtataa gttgagccaa ctaagtatgg gacagtagga aatgatacat cgataattaa
     1021 aaaagagccg tagaaaccgt aaaaatcatc ttcccaacca acaacatatg catacttccc
     1081 gttaacttta acatcaaatg cttttgttgg cgtgtaaaat cccccaatcc aggttgtatt
     1141 ataagattgc gaatgcagaa aggctgtaag taaagaagct aataaaacag taaaagcaaa
     1201 atttttcatt atccctccct gagaaccaat ttaataaact gtccactttt caaacatata
     1261 ggttggtata ttcataagca agatccgata taatattacc agtaatgttt tccaaattct
     1321 tgataatagg caactgtttg ttcaatacct tttttaaggt cagttttggg aacccaattt
     1381 aacatcttag ttattttcga aatattagtt atataatcgc cagtttcaac gtttaagtaa
     1441 tcctgcggcc agggttgata tttaatttta cctttaccaa ctatatcaac cacaagctgc
     1501 accatatccc ggaaggatgt tccagtccca cagccgatat taaatatttc gcttctaata
     1561 ttctcgtcta cacctgcaag aataaatgca tctgctaaat catctacata aatataatct
     1621 ctaatctgac taccatcacc aaatattgtt atctctttat cttccatagc gtttctaata
     1681 aaccagttga taattgcgta tttgctatgc tgcatctggc atcttggtcc ataaggattg
     1741 gcaatccgga atagaacaac agggatgtta aacacccgat tataatataa atatagattt
     1801 tcagcagcag ttttgttaac agcatacggt gttaatggat ttagtatatg atcttctgca
     1861 actggatttg aaataatctt gccaaactgt aacctagaac cggaatacaa tattttagct
     1921 gtaggattat tattctttac agcttctaat acattaagat gtccgatgta attaatatct
     1981 gtatccaaat aggggttttg aagactatcg ttgtgactaa cctgcccggc aagattaata
     2041 ataatatctt tatcctggac aagaacttta acagattctt tatcccgaat atcagaaata
     2101 tttattttta tgagttggct tatcttttca acattaaaaa tatttgcacc ataaggttta
     2161 ataagtgagt cgagcaaagt tacttcagca cccatcacca ctaatttatt tgcaagtgta
     2221 cttccgatca ttccaagacc acctgtaatt aatacttttt tattttttat ctttgtactc
     2281 attgttacct gcttttaatt aatattgaaa ttttaaatca cgcttttgat ctgaaaacaa
     2341 ataatttata aataaatgat aagttcaaaa caaagatata atttttgtac gatatttgat
     2401 tacagttatt ttgcaaaagg acttgcactc tactattctc taactaaagt atgtgattgc
     2461 catttatatg ttttcactcc ggatgagagg tgtaaaaaag cactacaaga aaaacaattt
     2521 caaaacctta caatcgttga ctttcatgaa gttgagaata aagaacttct cgaggtaaaa
     2581 agcaaaagag atgttgcaga atatttctgg acaataaaag gctcttgcct gcaatttctt
     2641 tttagtaaat ttaatttaga aattgcgaca tatatagatg cagatacatt tttctactca
     2701 tcaccggaac cattttttaa agagatggaa aacaaatctg ttttgatttt accgcataat
     2761 ttttcactca agtatcagca cgaaattgtt aatggggtat acaatgctgg atatatttca
     2821 tttagaaatg acaaggacgg attatctgca ctcaagtggt ggacggataa atctatagag
     2881 tggtgcttta agaaaaaaga aaagggaaag ttcgggg
//
LOCUS       DASWBY010000858         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_36794, whole genome shotgun sequence.
ACCESSION   DASWBY010000858 DASWBY010000000
VERSION     DASWBY010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36794"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1218)
                     /gene="recA"
                     /locus_tag="VF870_15485"
     CDS             complement(160..1218)
                     /gene="recA"
                     /locus_tag="VF870_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX9253647.1"
                     /translation="MASDREQKMKIIDDAIAGIEKTYGKGAIMKLGDGIVNDIEAIPT
                     GALSLDIALGIGGIPRGRIIEIYGPESSGKTTLCLHIIAEAQKMGGLAAFIDAEHALD
                     VNYAKRLGVDTANLLISQPDFGEQALEITDTLVRSNALDIIVIDSVAALVPRSEIEGE
                     MGDATMAVQARLMSQALRKLTGAISKSKTSVVFINQLRSKIGVMFGNPETTTGGNALK
                     FYASVRIDIRRIAAIKDGEDVIGNRTKVKIVKSKVAPPFKQTEFDILYNEGISKTGDV
                     IDMATELGIIKKGGAWFTYGEDRIQGREQFRQKLIEFPEMYKKIEIEIKTKLGLPGFK
                     TESTTEDKIEEKPKGKKK"
     gene            complement(1218..1778)
                     /gene="thpR"
                     /locus_tag="VF870_15490"
     CDS             complement(1218..1778)
                     /gene="thpR"
                     /locus_tag="VF870_15490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX9253648.1"
                     /translation="MKNRLFVSLNLPDNVIEQIISLRDKICDDKSIKWESKEKLHLTI
                     KFIGDVSGGIMNEISNELSFIIDYPSIKCSFNRFGFFYRDGKPIILWAGLNVDDLVND
                     LISELDDKLKKYSIPIEHRKFNPHITLLRIKKDPGISFVNNFKNFTFEPMLFTANSIT
                     LYKSVLHPNGSKYFEIKNYKLKELEK"
     gene            complement(1768..3024)
                     /locus_tag="VF870_15495"
     CDS             complement(1768..3024)
                     /locus_tag="VF870_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX9253649.1"
                     /translation="MKAYLISIGDELLIGQTINTNVSFIGNLISDINILIVKSAAIAD
                     DLPAILEELDLAASKADIIICTGGLGPTHDDVTRNAFVQYFKTELVHNNEVLEDIKSM
                     LKRHGRELKTTHEDQAMVPKIAQVIRNENGTAPGYWIEKDKKIFIVMPGVPYEMKAMM
                     SNHVIPKLIERIGEPKEFIKKVTLQTTGLPESILAERLGNIDELLKGAQLAFLPNQYG
                     VRLRVTTKSSSEEEANNHLLEIEQKIRSLVGRFIYGRGDENLEEVVGRLLKERELRIS
                     VAESCTGGGLADRITNVSGSSKYFERGIVTYSNAAKVELLRVNEDVILEKGAVSAEVA
                     LQMAEGIKSISGVDIGVSLTGIMGPTGAVTDKPVGTVFIGYCDDKVFTAKRFQFGENR
                     ILNKNRATQAALEMVRRNLLGIVLEE"
     gene            complement(3028..3168)
                     /locus_tag="VF870_15500"
     CDS             complement(3028..3168)
                     /locus_tag="VF870_15500"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX9253650.1"
                     /translation="MDIIKPFPARTSEKLPGGLGIMIDDVISGFYSLIILHLIVYTFG
                     VY"
BASE COUNT          932 a          651 c          454 g         1134 t
ORIGIN      
        1 ccccgttatc aaaatagacc gatacattct tttcgtcttt acgggaaact ctttcaattt
       61 tcatttatga ttatccgcac tcattatcta tgaatattaa aaaatggatt ccgaatcaag
      121 tccggaatga caacatatgg attatatgga ttacttctac tatttctttt tcccttttgg
      181 tttttcttct attttatctt ctgtagtgga ttcagtttta aatcctggca aaccaagttt
      241 tgtttttatt tcaatttcaa ttttcttata catttccgga aattcaataa gtttctgtct
      301 gaattgttcc ctgccctgaa ttcgatcttc accataagta aaccaagccc cgcctttttt
      361 gataattcca agttctgttg ccatatcaat cacatcgccg gttttgctta taccttcgtt
      421 gtacaaaata tcaaattcag tttgtttaaa tggaggagca actttacttt taacaatttt
      481 aactttagtt ctgtttccaa taacatcttc accatcttta atagctgcaa ttcttctgat
      541 atcaattctg acagaagcat aaaacttaag tgcatttcca ccggtagtag tttccggatt
      601 tccaaacatt acaccaattt tacttctaag ctgatttata aatactacag atgtttttga
      661 ttttgaaatt gcaccggtca attttcttaa agcttgtgac atcaatcttg cctgaactgc
      721 cattgttgca tcacccattt caccttcaat ttcagaacgc ggaacaagcg ctgctactga
      781 atctataacg atgatatcta atgcattgct gcgtactaaa gtatcggtaa tttctaatgc
      841 ttgttcacca aaatcgggtt gtgatataag aagatttgca gtatcaacac cgagacgctt
      901 tgcataatta acatcaagag cgtgttcagc atcaatgaat gcagctaatc cacccatctt
      961 ttgagcttca gcaataatgt gaagacataa tgtggtttta ccacttgatt ccggcccgta
     1021 aatttctatt attcttccgc gtggaattcc tccaatgccg agagcaatat ctaaagacaa
     1081 tgctccggtt ggaattgctt ctatatcatt tacaattcca tcaccaagtt tcataattgc
     1141 gcctttgccg taagtttttt ctattccggc aattgcgtca tcaataattt tcattttttg
     1201 ttctctatca gaagccatca tttctccagt tcttttagtt tataattttt gatttcaaaa
     1261 tattttgatc cattgggatg cagaacactt ttataaagcg ttattgaatt agcagtaaaa
     1321 agcattggtt caaaagtaaa atttttgaag ttgttaacaa agctaattcc tggatctttt
     1381 tttattctta ataatgttat atgcggatta aattttctgt gctcgattgg aatagaatat
     1441 ttctttagtt tatcatccaa ttcagatatt aaatcattaa ccaaatcatc tacattcaat
     1501 cctgcccaaa ggattattgg cttaccatca cgataaaaaa atccaaacct attaaacgaa
     1561 cattttattg acggataatc aatgataaaa ctcaattcgt ttgagatttc attcattatc
     1621 ccgcctgaca catctccaat aaattttatt gtgagatgaa gtttctcttt agattcccat
     1681 tttatacttt tatcatcaca aatcttatca cgcagactga taatctgttc aattacatta
     1741 tcaggtaagt taagtgaaac aaaaagtcta ttcttcaaga acaattccca ataaatttct
     1801 tctaaccatt tcaagtgcag cttgtgttgc cctgttttta ttcaggatac ggttttcgcc
     1861 aaactgaaat ctttttgctg taaaaacttt atcatcgcaa tatccgataa agacagttcc
     1921 aacaggttta tcagtcacag cgccggtagg tcccataatt ccggttaatg aaacaccaat
     1981 atctactccg ctaatagatt ttatcccttc agccatttgt aatgcaactt ctgccgacac
     2041 agcacctttc tcgagtatca catcttcatt aactctaaga agttccactt tagcggcgtt
     2101 gctgtaagtg acaattcccc gctcaaaata tttactgctt ccactaacgt tggtaatgcg
     2161 atctgctaat ccgccacctg tgcaggattc tgcaactgaa attcttaatt ctctttcttt
     2221 taaaagtctt ccaacaactt cttctaaatt ttcatcacca cgaccataaa taaaccggcc
     2281 aactagactt ctaatttttt gttctatttc taaaaggtga ttatttgctt cttcctcgct
     2341 gctggatttg gttgttactc ttaacctaac tccatattga tttggtaaaa aggctagctg
     2401 agctcctttt aaaagttcat caatattgcc taagcgttct gctaaaattg attccggtaa
     2461 cccggtggtt tgaagtgtaa ccttttttat aaattctttt ggctcgccaa ttctttcaat
     2521 cagttttgga ataacatgat tgctcatcat agctttcatt tcataaggaa cacccggcat
     2581 aacgataaat atttttttat ccttttcaat ccagtaaccg ggtgcggttc cattttcatt
     2641 acggataacc tgagcaattt tgggaaccat agcttgatct tcgtgagttg tttttaattc
     2701 tcttccatga cgcttaagca tgctttttat atcttccagg acttcattgt tatgcactaa
     2761 ttcagtttta aaatactgca caaaagcatt ccttgttaca tcatcgtgag tcggccctaa
     2821 tccgccggta caaataataa tatctgcctt tgaggcagct aaatcaagtt cctctaagat
     2881 tgctggtaaa tcgtctgcta tagcggcaga tttaactatt aatatattaa tatcagagat
     2941 cagatttcca ataaaagata catttgtatt aatcgtttgc ccgattaata attcatcccc
     3001 gatggaaatt aaataagctt tcatttttta ataaactccg aatgtgtaaa caattaagtg
     3061 aagaataatt aaggaataaa aacctgaaat gacatcatca atcattatcc ctaacccgcc
     3121 cggtaatttt tcagaagttc ttgccggaaa cggtttaata atatccagcg c
//
LOCUS       DASWBY010000859         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_73659, whole genome shotgun sequence.
ACCESSION   DASWBY010000859 DASWBY010000000
VERSION     DASWBY010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73659"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VF870_15505"
     CDS             <1..1235
                     /locus_tag="VF870_15505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9253651.1"
                     /translation="MVYFDREKMETIIKNLLSNAFKFTPNKNEISFSLLETEESSFEI
                     KVKDTGIGISEEQLPKIFNRFYQVNGTHTREHEGTGIGLALVKELVELHHGSISVDSK
                     LGEWTEFKIILPLGKDHLAPQEIVEIDKQILPETLIVDKTEFVNSTYHNSSVENPITS
                     KNIILIVEDNADVREFIKDSLGNEYHFEEAENGEEGIIKAEEIIPDLIISDIMMPKID
                     GNEMTRRLKSDQKTSHIPIILLTAKSGQENKIEGLETGADDYLTKPFDAKELHIRINN
                     LINLRKKLQEIYGTEKITSVTKSKHKLKAIDAQFMERLLKVIEEHISEEEFTIENFGH
                     EVGMSRSQMFRKIKALTGKSCSVYLRSVRLAKAKIMLQNREATVSEIAYSVGFGSPSY
                     FAHCFKEEFGYAPSDLVS"
     gene            1421..1849
                     /locus_tag="VF870_15510"
     CDS             1421..1849
                     /locus_tag="VF870_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253652.1"
                     /translation="MKKLFLCLVVLFAFQICLLSQTPAELQNSGFNRVQIEDNYLAGL
                     NSDVPGLQVSAAYYLGEMKSERAVIPLMKMFRDENNAPGQRLMAAWSLLKIGDERGIY
                     LIKCQSEDQNCDYLRCLCEYFYKDHNLKAHSSINPYENLK"
     gene            2320..>2569
                     /locus_tag="VF870_15515"
     CDS             2320..>2569
                     /locus_tag="VF870_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253653.1"
                     /translation="MKNLFLSLALLFLIVFTACQENTITNPITNENIGKDINYYNNLS
                     SNTIKLNTSLLDPSQNPNIAFQIIGTIEYGVNYMPHQLS"
BASE COUNT          957 a          379 c          426 g          807 t
ORIGIN      
        1 tgatggtgta tttcgataga gaaaaaatgg aaacaattat taaaaatctt ctttcaaatg
       61 cttttaaatt cacccccaat aaaaatgaaa tatcgttttc acttttagaa acagaggaaa
      121 gcagctttga gataaaagtt aaagatactg gaattggaat atccgaggaa caattaccga
      181 aaatatttaa tcggttctat caggttaatg gaacacatac ccgtgaacat gaaggcacag
      241 gcataggttt ggctctggtt aaagaactgg tggaacttca tcatggttca atttctgttg
      301 atagtaaatt aggtgaatgg accgaattta aaatcattct tccgcttggg aaagaccatc
      361 ttgcacctca ggaaattgta gaaatagata agcagatact gcctgaaact ctgatcgttg
      421 ataaaactga atttgttaat tcaacatatc ataattccag cgttgaaaat ccgattacaa
      481 gcaaaaatat tattcttata gttgaagaca acgcagatgt aagagaattt attaaagatt
      541 cacttggaaa tgaatatcat tttgaagaag ccgaaaacgg tgaagaagga attattaaag
      601 ctgaagaaat tattcccgat ttaattatca gcgatattat gatgccgaag atagatggaa
      661 atgaaatgac ccgaagatta aaatcagatc aaaaaacgag tcacattcca attattctat
      721 taacggcaaa atccggacaa gaaaataaaa tagaaggatt agaaacaggt gcagatgatt
      781 accttacaaa accttttgat gcaaaggaac ttcatatcag aattaataat ctaattaatc
      841 ttagaaaaaa acttcaggag atttacggta ccgaaaaaat tacatccgtt acaaaatcaa
      901 aacataagtt aaaagcaata gatgctcagt ttatggagag attattaaaa gtaattgaag
      961 agcatatttc tgaagaagaa tttacgattg aaaattttgg tcatgaagtt ggaatgagcc
     1021 gttctcagat gtttagaaaa ataaaagcct taactggtaa atcctgcagt gtttatttaa
     1081 gatctgtaag attagcaaaa gctaaaatta tgcttcaaaa tcgtgaagcg accgtttcag
     1141 aaatcgcata ttcggtcgga tttggaagcc catcgtattt tgcccattgc tttaaagaag
     1201 aatttggtta tgccccgagt gatctggtta gttaaaaatt tctaccaccc aaaatccttt
     1261 tatttaacat ttgattttta ttaaaattta attcccgcta aaacttgcaa catagttgct
     1321 aatctttgaa acatatctga taggtagccc ccttcgtttt tttgtttatt tacagtgttc
     1381 tttaaatgaa aatttaataa acaaacaaaa ggagaaggaa gtgaaaaagc tatttttatg
     1441 tttagtagtt ctttttgcat ttcaaatctg tttgctttca caaacacctg cagaattgca
     1501 aaattccggt tttaaccgtg tacaaataga agataattac cttgccggac ttaattctga
     1561 cgtccccggt cttcaagtaa gtgcagcata ttatcttgga gaaatgaaat cagaacgcgc
     1621 agttattcca ttaatgaaaa tgtttagaga tgaaaacaat gctcctggtc agagattaat
     1681 ggctgcttgg tcactgttaa aaattggtga cgaacgtggt atctatttaa taaaatgtca
     1741 aagtgaggat caaaactgtg attatttaag atgcttgtgc gaatactttt ataaagatca
     1801 caacttgaaa gctcattcaa gcattaatcc gtacgaaaat ttaaagtaat ttttttctaa
     1861 actaaaacgg actggctagt gaaagattta aaaaatgata aaaaataact tgggggaaaa
     1921 tttaataacg ttaagaaaga ttgaattaga aataaaattc tttcattacc aaattaaaag
     1981 acatattaat ttgctatcaa aaacctgctt ttattcctcg cgttgctttt cctgaacttt
     2041 tgtctacaga aattaaaaaa atgaaactat taagataata gaacttttta atcctattat
     2101 acaaaataac agtgattcaa atgagtcgtt tttataatta atcatgaaat aacgtagaat
     2161 taaaattgag caaatatcgt tcattttgca ggtattagaa acttgcaaca tagttgctaa
     2221 tctttgaaac atatctgata ggtagctccc ttcgtttttt tgtttatttg cagtgttctt
     2281 taaatgtaaa atttaataaa caaacaaaag gagcctgcaa tgaaaaacct atttttatcc
     2341 ctcgcattac tttttctgat agtttttact gcctgtcagg aaaacacgat tacaaatcct
     2401 atcacaaatg aaaatattgg taaggatata aattattata acaatctgag cagtaataca
     2461 ataaaattaa atacgtcgct tttggatcca agccaaaatc ctaacatagc ttttcaaatt
     2521 atcggaacaa ttgagtatgg tgttaattat atgccgcacc agttaagtg
//
LOCUS       DASWBY010000860         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_370639, whole genome shotgun sequence.
ACCESSION   DASWBY010000860 DASWBY010000000
VERSION     DASWBY010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370639"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VF870_15520"
     CDS             <1..1479
                     /locus_tag="VF870_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253654.1"
                     /translation="PANKVAGPSFGNVGYDIVDQSQLTNDKYQVTFFKDSSSSSYSTF
                     WRLKNLTTGQTLVDSSLDYLYSDPAYIAVKSTDGFIIRVEEQIATLGTPNYTPSSNVW
                     YDNFDDASATGVFYVGKDIPQGTAVFNFRNRQSDYISADRLRKVELRFGTQGKAYRYL
                     NNYIGSSNLSRLNAYTFAGAVTAADTIGKGTVGNWNTTTDRANGYVDVPFTAWVVDDR
                     YPNDSRQLAVGFVERRVSNNFPGGNPDGNWDPGDSLNTSGEVIIIFDASYDQNGQQIE
                     YTGGEFNTPGGPVTVWSDLLRATTGLQTPPADAIGFTPEQRKIYLSPWFNAMYVVGLQ
                     KKDASMFFNNGDVLSTNITVYPYTSEDIYEFTTSSTTLNEDQQKALFEKVNVYPNPLY
                     GFNVATSYTNSSADEPFVTFSNLPEEVSIKVYSLSGQLLRTLGVEDKTSPTSPFLTWN
                     LQNESGLRVASGMYFAIVESPKYGEKILKFAIIMPQKQIQKY"
BASE COUNT          510 a          255 c          325 g          523 t
ORIGIN      
        1 cctgcaaaca aagttgctgg tccatctttc ggtaacgttg gttacgatat tgtggatcaa
       61 tctcaattaa ctaatgataa atatcaggta acattcttta aggattcttc atcctccagt
      121 tattctacgt tttggagatt aaagaattta acaactggtc agacattagt tgattcatct
      181 cttgattatc tctattcaga tcccgcttat attgcagtta aatcaactga tggttttatt
      241 ataagagtag aagaacaaat tgcaacatta ggaactccta attatactcc aagctctaat
      301 gtttggtatg ataattttga tgatgcctct gccacaggtg tattttatgt aggaaaagat
      361 attccacagg gtacagcagt atttaatttc agaaatcgac agtcagatta tatctcagct
      421 gatcgattga gaaaagtgga gctaagattt ggaactcagg gcaaagctta tcgatatttg
      481 aataattata tcggcagttc aaacctatca aggttaaatg cctatacatt tgcaggtgcc
      541 gttactgcgg ctgatactat tggcaaagga acagttggta attggaatac aacaactgat
      601 cgtgcaaacg gatatgttga tgtaccattt actgcctggg tagtagatga cagatatcct
      661 aacgatagca gacagcttgc tgtagggttt gttgaaagaa gagtctcaaa taatttccct
      721 ggcggcaatc ctgatggtaa ctgggacccg ggtgattcac ttaatacctc aggtgaagta
      781 atcattattt ttgatgctag ttatgatcaa aatggacagc aaatagaata tacaggtggt
      841 gaatttaata ctcctggtgg tccggtgaca gtatggtcag atttgctaag agcaacaacg
      901 ggcttacaaa cacctccagc tgatgctatt ggatttactc cggaacaaag gaagatatat
      961 ttatctcctt ggtttaatgc gatgtatgtt gtaggattgc agaagaaaga tgcttcaatg
     1021 ttttttaaca atggagatgt tctaagtaca aatattactg tgtatcctta taccagtgaa
     1081 gatatttatg aatttaccac ttcttctaca acgttaaatg aagatcaaca aaaagctttg
     1141 tttgagaagg taaatgttta tcctaatcct ctgtatggtt ttaatgtggc tacaagttat
     1201 actaatagtt cggcggacga accatttgta acattttcaa atcttcctga agaggtatca
     1261 attaaagttt attcgttgtc gggtcagttg ttgagaactt tgggtgttga agataaaact
     1321 tcaccaactt ctccgttctt aacctggaat cttcaaaatg aatctggatt aagagtagct
     1381 tcagggatgt attttgcaat tgtagaatca ccgaaatatg gtgaaaagat tctgaagttt
     1441 gctataatta tgcctcaaaa acagattcaa aaatattagg attattgggc ggtattatac
     1501 cgcccggtaa attttgaaaa aatttaggag tattagaaaa tgaaaaaaat tgtcacaata
     1561 attcttttag caatgttttc tgcttcagtt tatgcaggtg atgttgccag gaa
//
LOCUS       DASWBY010000861         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_111247, whole genome shotgun sequence.
ACCESSION   DASWBY010000861 DASWBY010000000
VERSION     DASWBY010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111247"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VF870_15525"
     CDS             <1..374
                     /locus_tag="VF870_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX9253655.1"
                     /translation="LAATLLCAPRSRMDILTNDELNNKINASKLVGKYSEVIDRESAY
                     EILNKKLETSREFDLQVPDSGKYKYEPQPSGKTSTRTRTEKSTVDKVINSPLSKQIGR
                     TLAREISRGILGVLGFGGRKR"
     gene            complement(387..1133)
                     /gene="nagB"
                     /locus_tag="VF870_15530"
     CDS             complement(387..1133)
                     /gene="nagB"
                     /locus_tag="VF870_15530"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX9253656.1"
                     /translation="MLVLIREDYAQVSLEASLQIASQIKMKPDSVIGFATGSTPLGLY
                     KELIRMHKDEGLDFSKVTSFNLDEYVGLPPSHPESYHYFMWENLFKHVNINPSNVNIP
                     MGMADNIEIFCNWYEEKIKSHGGIDIQILGIGANGHIAFNEPGSSLGSRTRIKTLGEK
                     TRLDNSRFFNNLDEVPKYAITMGVGTIMEAKKLLLLANGLNKAEAIKSTVEGPIRAKY
                     PATIVQYHRYATIIIDKEAASMLEGEYIDK"
     gene            1310..>1819
                     /locus_tag="VF870_15535"
     CDS             1310..>1819
                     /locus_tag="VF870_15535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253657.1"
                     /translation="MKSIFITILSLCFAFTIKSQTIFITGNIKDVKTNSPIDNAKVQI
                     KNISNGLIDSVFSDAVGNWQYDIATSVEDDIQYLPTTLEVLQNFPNPFNPSTKIGFLI
                     PADENVSIMVHNILGELVDVKSSFLTKGNYVVDWFSKGSAGVYFYTISYGNQSITKKM
                     IQLDGGNGIG"
BASE COUNT          611 a          309 c          291 g          608 t
ORIGIN      
        1 cccttgcggc aacattacta tgtgcaccta gatcgcgtat ggatatttta acaaacgatg
       61 agttaaataa taaaatcaat gcttcaaaat tagttggcaa gtacagtgaa gtaatagatc
      121 gcgagagtgc ttacgaaatt ctcaataaaa aattagaaac ttcaagggaa tttgatttac
      181 aagttccgga ttctgggaaa tataaatatg aaccacagcc atcaggaaaa accagtacaa
      241 gaacacgaac cgaaaaaagt actgttgata aagttattaa tagtccgctt tcaaaacaga
      301 ttggcagaac tttagcgaga gaaatttccc gcggaatcct cggcgtgctg ggttttggtg
      361 gaagaaagag ataatctaca agtgatttat ttatctatat actccccttc caacattgat
      421 gctgcttctt tatcaattat gatagttgca tacctatggt actgaacaat tgttgcagga
      481 tactttgccc taataggacc ctctacagtt gactttattg cttcggcttt gtttaacccg
      541 tttgccagca ataaaagttt ctttgcttcc ataatagtac caacacccat agtgattgcg
      601 tacttaggaa cttcatcaag attattaaag aatcttgaat tatctaatcg tgttttttct
      661 ccgagagttt ttattcttgt ccttgagcct aaagaagagc ctggttcatt gaaagcaata
      721 tgtccattag caccaattcc taaaatttga atatcaattc cgccatgact ttttattttt
      781 tcttcatacc aattgcagaa tatttcaatg ttatcagcca ttcccattgg aatattaaca
      841 tttgatggat tgatattaac atgcttaaac agattctccc acataaaata atgataactc
      901 tcaggatgtg aaggcgggag tccgacatat tcatccagat tgaatgatgt aactttagaa
      961 aaatccaaac cttcgtcttt atgcattcga attaattctt tatataaacc caaaggtgtg
     1021 cttccggtag caaatccaat aactgaatca ggtttcattt ttatttgaga tgcgatttga
     1081 agggaggctt ctaaacttac ttgagcgtaa tcttctctta tcaatactag cataatatct
     1141 cctactgata attatttttt gttataataa aattagaata aaattagttg tgattaaata
     1201 atttgataaa aaatttttat agaagaacca gttagtgctt aaatctaaaa tgcttatttt
     1261 ataagcaaat ttatatcgct ataattgaat taatattatt gaaagtctta tgaaatctat
     1321 cttcattaca atcttaagtt tatgttttgc ttttacaatt aaatcacaaa caatttttat
     1381 tactggaaat attaaagatg taaagaccaa ctcaccaatt gacaacgcta aagtacagat
     1441 aaaaaatatt tctaatggac tgattgattc cgtattttct gatgcagttg gtaactggca
     1501 atatgacata gcaacttctg ttgaagatga tattcagtat cttccaacca ccttagaagt
     1561 actgcaaaat tttcccaacc catttaatcc ctcaactaaa attggttttt taattcccgc
     1621 tgatgaaaat gtttcaatta tggttcacaa cattctaggt gaactggtcg atgttaaaag
     1681 ttcatttctt accaaaggca attacgttgt tgattggttc agcaaaggaa gtgcaggagt
     1741 ttatttctac acaatttcat atggaaatca atctataact aaaaagatga ttcagcttga
     1801 tggcggcaat ggaatcgga
//
LOCUS       DASWBY010000862         5111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_562431, whole genome shotgun sequence.
ACCESSION   DASWBY010000862 DASWBY010000000
VERSION     DASWBY010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562431"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VF870_15540"
     CDS             complement(<1..721)
                     /locus_tag="VF870_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9253658.1"
                     /translation="MTENSIINISLAILFLPLLGFVVTLLLGKKVKSIYLFEVFLISV
                     VLVASIVLVFNKLSYFIDDKIVSELEWFRLSDTLVIKLGFLLDNITVLMLFVVALISA
                     LVHYFSIAYMKGDERYNRYFAYLGIFTFSMNGIVLTNNILMMYIFWELVGLSSYLLIG
                     FWFEKKSAADAGKKAFLVNRIGDVGMFAGILILFLTYNTFSFDTIFQSISAGKLPFNS
                     DFWLTVTGILLFMGAIGKSAQF"
     gene            complement(725..1036)
                     /gene="nuoK"
                     /locus_tag="VF870_15545"
     CDS             complement(725..1036)
                     /gene="nuoK"
                     /locus_tag="VF870_15545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX9253659.1"
                     /translation="MIEVGLNHFLVVSTFLFAMGIYAIVTRKNAIMVLMGVELILNSA
                     NINFIAFTRFGNFGLQGHLIALFVIVLAAAEAAIALAIVLNIYKTLSTVNIDEVDSLK
                     D"
     gene            complement(1033..1527)
                     /locus_tag="VF870_15550"
     CDS             complement(1033..1527)
                     /locus_tag="VF870_15550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9253660.1"
                     /translation="MNVYDLIFYLFAAITVLSAFFVVTNRNIVYSAFFLLFTFFGVAG
                     IYVLLGADFVAIVQIIVYIGGILILLLFGVMLTNKITNVEIKSGTINIYPAVIGVGLL
                     SGALLAALITSSWKTFPTEAPLPTTAALGKMLLQDYALVFELLGIILLIALIGAASIA
                     RRKA"
     gene            complement(1529..2095)
                     /locus_tag="VF870_15555"
     CDS             complement(1529..2095)
                     /locus_tag="VF870_15555"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX9253661.1"
                     /translation="MKEYFGNIYTALVTIFIGMKITFKHLFTPAVTIQYPEVKPQLPE
                     RERNRLYVNMDDCIGCDQCSRACPVSCIEIETTKSIPGEDLGKTSNGKKKALWVTNFT
                     IDFAKCCYCQLCVFPCPTDCIYMTDVYEFSEWDREKLIYDFATLTPAERQEKRDNLAK
                     FNAEKEAEKLKAASEPKPEIKKDQPEGK"
     gene            complement(2095..3162)
                     /gene="nuoH"
                     /locus_tag="VF870_15560"
     CDS             complement(2095..3162)
                     /gene="nuoH"
                     /locus_tag="VF870_15560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX9253662.1"
                     /translation="MFDFFYNLTNSVIISGLLISVFPLTFILLYVIFAVYLERKVSGH
                     MQDRLGPMRVGYHGALQLIADMLKLIQKEDIVAKDNDKLLFNLAPIIVFTGSFAAMAA
                     IPLSSALFGANIDLGIVYIVAITGLGVAGILMAGWSSNNKYSLLGSMRSVAQIISYEI
                     PTLLVILSIIMITGTMNLNTLSEMQTSSIWNWMIFGGPGTGISKYLLIPFMFIGFLVI
                     YISTLAEVNRTPFDIPEAESELVSGYHTEYSGMKFAMFFLSEYANMFIVSAIVVALFL
                     GGYQSPFGYLGNLFHAPWLIPIEQFFWFAFKGIFFVFVQIWLRWTLPRLRVDQLMTVS
                     WKYLIPIAFVNLIIIGLITLL"
     gene            complement(3162..4310)
                     /locus_tag="VF870_15565"
     CDS             complement(3162..4310)
                     /locus_tag="VF870_15565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX9253663.1"
                     /translation="MSQDLITNTKSSGLRTEEMVLNMGPQHPSTHGVLRLELTLEGEI
                     VTKVMPHIGYLHRCFEKHCEAMTYPQVVPYTDRMDYLAPMGNEFGYAIAVEKLLGIQV
                     PERVEYIRVIMAELQRIASHLVAIGTYGIDIGAFTPFLYCFRDREKILNLFEITCGAR
                     LLYNYIWVGGLSHDLHPDFVRKTREFIKEFRPTIKEVNELLSYNKIFIDRTGGVGVLP
                     VETAVNFGVSGPNLRGSGMKWDIRKDDPYSIYHKFDFDIPVGDGRNGAVGDCWDRYFV
                     RVLEMEESLKIIEQALDQLPEGDVQSAIPKRIKPPIGHVYTRVENPKGELGYFIISDG
                     STSPYRIKVRGPSFVTLQIMDVLCKGHMVADIIAILGSIDIVLGEVDR"
     gene            complement(4312..4800)
                     /locus_tag="VF870_15570"
     CDS             complement(4312..4800)
                     /locus_tag="VF870_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9253664.1"
                     /translation="MKKAEEILQILKEKFPDSTLELKNDQPTEAVLIASPIEIDKICS
                     FLRDSSELLFDSLMNLSGVDEPKDSLLSVYYHLESTKLRHKITLKVSTDRNKPEVATV
                     TEVWKGADWHEREAYDLFGIIFLNHPDLRRILMPYDWDAGYPLRKDYENPEFYQGMKV
                     PY"
     gene            complement(4797..>5111)
                     /gene="nuoB"
                     /locus_tag="VF870_15575"
     CDS             complement(4797..>5111)
                     /gene="nuoB"
                     /locus_tag="VF870_15575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX9253665.1"
                     /translation="IPRPSPRQADIIIISGTVTLKMATRIKRLYEQMPDPKYIISMGS
                     CSNCGGPYWEHGYHVLKGVDRVIPVDVYVPGCPPRPEALLEGLLKLQEKIRNESMIKK
                     TA"
BASE COUNT         1725 a         1002 c          823 g         1561 t
ORIGIN      
        1 ggaactgagc tgattttcca attgcgccca taaataataa aatgccggtt acagttagcc
       61 agaaatcaga gttaaatgga agtttgcctg cgctaatgga ttgaaagatt gtatcgaagg
      121 aaaaagtgtt ataggtaaga aataaaatta aaatacctgc aaacattcca acatcaccaa
      181 tcctgtttac taagaatgct ttcttacccg catcggcagc ggactttttc tcaaaccaga
      241 atccaatcaa aaggtaagat gataatccaa ccagctccca gaaaatgtac atcatcaaaa
      301 tattattggt tagtacaatt ccattcatag agaaagtaaa gattccaaga tatgcaaagt
      361 aacgattgta tctttcatcg cctttcatat aggcgataga gaagtaatgc accagtgcgc
      421 taatcaaagc aacaacaaac aacatcagca cggtaatgtt atccaacaaa aacccaagtt
      481 taataactaa tgtatcacta agcctgaacc attccagttc ggatacaatt ttatcatcaa
      541 taaaatatga cagtttatta aaaacaagta caattgaagc gacaagaaca acagaaatta
      601 gaaatacttc aaacagataa attgacttta cttttttgcc gagcaaaagt gtaacgacaa
      661 atccgagcag cggtaaaaat aaaattgcaa gcgaaatatt tattatactg ttttctgtca
      721 tttactaatc tttcaagcta tctacttcat ctatatttac tgttgataaa gtcttataaa
      781 tattcaatac aattgctaaa gcgattgcag cttcagcagc agcaagtaca atcacaaata
      841 acgcgattaa atgtccttgt aatccaaagt ttccaaaacg tgtaaaggcg ataaagttta
      901 tgttagcaga attgagtatc agttccacgc ccatcaatac catgattgca ttttttcttg
      961 taacgatggc gtaaattccc atagcgaata aaaatgtgct aacaactaaa aaatgattca
     1021 atccaacttc aatcatgctt ttcttctcgc aatcgatgct gcaccaatta gtgcaatcaa
     1081 aagaataatt cctaacagtt caaatacaag agcataatcc tgcaaaagca ttttaccaag
     1141 tgctgctgtt gttggcagcg gtgcttctgt tggaaatgtt ttccagctag aagtaattaa
     1201 agcagccaat aaagcacctg aaagcaatcc aactccaatt acagcggggt aaatattaat
     1261 tgtacctgat tttatttcaa catttgttat tttattagtt agcatcacac caaataataa
     1321 taaaattaaa attccaccta tgtacactat aatttgaacg atggcaacga aatcggcacc
     1381 gagcagcaca taaatacctg caacaccaaa gaatgtaaac aaaaggaaaa acgctgagta
     1441 aacaatattc ctgttagtaa caacaaagaa tgccgataaa acagtgattg cagcaaacaa
     1501 gtaaaatatc aaatcgtaaa cattcacttt attttccctc tggctgatct tttttaattt
     1561 ctggtttcgg ttcagatgct gcttttaatt tttctgcttc tttttcagca ttaaactttg
     1621 caaggttatc tcttttttcc tgtcgttctg caggagtcag agttgcaaaa tcataaatta
     1681 atttttctct atcccactca gaaaactcat atacatcggt catataaata cagtctgttg
     1741 gacaagggaa aacacataac tggcagtagc agcattttgc aaaatcaata gtaaagtttg
     1801 ttacccagag tgctttcttt tttccgtttg atgttttgcc cagatcttca ccaggaatag
     1861 attttgtcgt ttcaatttca atacaactaa caggacaggc tcttgagcat tgatcacagc
     1921 ctatgcagtc atccatattt acgtaaagcc tgttgcgctc tctttcagga agttgaggtt
     1981 taacttctgg atattgaata gttacagccg gagtgaagag atgtttgaat gtaatcttca
     2041 ttccaataaa gattgtaaca agagctgtgt atatatttcc gaaatattct ttcattataa
     2101 aagtgtaatc aatccgatta ttataagatt aacaaatgca attggaatta aatatttcca
     2161 actcactgtc attaattgat caacgcgtaa tcttggcaaa gtccatctaa gccatatctg
     2221 aacaaaaaca aaaaatatac ctttgaatgc gaaccagaaa aactgttcaa ttggtatcag
     2281 ccagggtgca tgaaaaagat ttccaaggta tccaaaaggt gattgatatc cgcctaagaa
     2341 caacgcaaca acaatagctg atacaataaa catatttgca tattctgata aaaagaacat
     2401 tgcgaatttc attcctgaat attctgtatg atatccggaa acaagttctg attcagcttc
     2461 gggaatatcg aatggagttc tattaacctc agcaagagtg cttatgtaga tgactaaaaa
     2521 gcctatgaac ataaaaggta ttaaaaggta tttacttatt ccggttccag gcccaccaaa
     2581 tatcatccaa ttccaaatac tgcttgtttg catttcactt agagtattaa gattcattgt
     2641 acctgttatc ataataatac ttaaaatcac aagtaatgtt ggaatttcat aactgattat
     2701 ttgagctact gaacgcatag aacctaataa tgagtattta ttgttcgatg accagccagc
     2761 cattagaatc ccagcaacac ctaatccagt aattgcaacg atgtagacaa ttcctaaatc
     2821 aatattagct ccgaataaag cgctgcttag cggtatagca gccattgcgg caaaagatcc
     2881 tgtaaacaca atgataggtg caagattaaa aagaagttta tcattatcct tagcaacaat
     2941 atcttctttc tgaataagct tcagcatgtc tgcaataagt tgaagtgctc catgataacc
     3001 tactctcata ggaccgagtc tatcctgcat atgcccggaa acttttcttt cgagatacac
     3061 agcaaagatt acataaagca aaataaaggt tagcggaaaa acgctaatta ataagcctga
     3121 aattataacg ctgtttgtta gattataaaa aaagtcaaac attatctgtc aacctctccc
     3181 aaaactatat caatgctgcc aagaattgct ataatatctg caaccatatg acctttacac
     3241 aagacatcca ttatttgtaa agttacaaag gaaggaccac ggacttttat tcgatatgga
     3301 cttgtagaac catcactaat aataaagtag cctaattcac ctttgggatt ttctactcgc
     3361 gtataaacgt gaccaattgg aggttttatt cttttaggta ttgctgattg aacatctcct
     3421 tcaggcaact ggtccagagc ttgctcaata atctttaagc tctcttccat ttcgagtacg
     3481 cgaacaaaat atctgtccca gcaatcaccg actgctccat tacgtccgtc tccaacagga
     3541 atatcaaaat caaatttatg atagatagaa taaggatcat cttttctaat atcccacttc
     3601 attcctgatc ctctgaggtt tgggccactg acaccaaaat tcacagctgt ttcaaccggc
     3661 aaaactccaa cgccaccggt tctatcaata aaaattttat tataacttaa taattcattt
     3721 acttctttaa ttgtaggcct gaattctttg ataaattccc tggtttttct aacaaaatcc
     3781 ggatgcagat cgtgtgataa tccaccgacc cagatatagt tataaagcag ccttgcacca
     3841 caagtgattt caaataggtt aagtattttt tcacggtctc taaaacaata caagaatggt
     3901 gtaaatgcac ctatatcaat gccgtaagta ccgattgcaa caagatgtga agctatacgt
     3961 tgaagttctg ccattattac tctaatgtat tcaactcttt caggaacttg aattcctaaa
     4021 agtttttcca cagcgatggc ataaccaaat tcatttccca tcggtgcaag ataatccata
     4081 cgatctgtat aaggaactac ctggggataa gtcattgctt cacaatgctt ttcaaaacag
     4141 cggtgcaagt aaccgatatg gggcattacc tttgtaacta tttcgccttc aagggtcaat
     4201 tcaagcctaa gcacaccgtg tgttgacgga tgctggggac ccatattgag gaccatttcc
     4261 tctgttcgca aacctgagct tttagtattt gtaattaaat cttgagacat cttaataagg
     4321 tactttcatt ccctgataga attcaggatt ttcataatct tttctaagtg gataaccggc
     4381 atcccaatca tatggcatta atattcttct taagtctgga tgattaagga agattatccc
     4441 gaataaatcg tatgcttcac gttcgtgcca atctgcacct ttccaaactt ctgtaactgt
     4501 tgcaacttca ggtttatttc tgtctgtaga aacttttaaa gtaattttat gtctaagttt
     4561 tgtagattct aaatgataat aaacgcttaa taaagaatct ttgggctcat caacgccaga
     4621 taagttcatt aaagaatcga ataacaattc tgaagaatcc cgaaggaaac tacatatctt
     4681 atcaatttca atcggagaag ctattaaaac tgcttctgtg ggttgatcat ttttcaattc
     4741 cagtgtagag tcaggaaact tttcttttaa aatctgtagg atttcttcgg cttttttcat
     4801 gcagttttct ttatcatact ttcattacgg attttttcct gaagctttaa taaaccctca
     4861 agtaatgctt ccggtctcgg aggacatccc ggcacataaa catctaccgg aataactcta
     4921 tcaacacctt ttaaaacatg ataaccgtgc tcccagtatg gtccgccgca atttgagcag
     4981 cttcccatag agataatgta cttaggatca ggcatttgtt catataatct tttaatacgg
     5041 gttgccattt ttaaagtaac tgttccggag ataataataa tatcagcttg tctgggagaa
     5101 gggcggggaa t
//
LOCUS       DASWBY010000863         3038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_150239, whole genome shotgun sequence.
ACCESSION   DASWBY010000863 DASWBY010000000
VERSION     DASWBY010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150239"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VF870_15580"
     CDS             <1..907
                     /locus_tag="VF870_15580"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX9253666.1"
                     /translation="INSFLQFPAASQLTFLLAILMIFAGIGFKISIVPFHFWTPDVYE
                     GAPISITAFLSVASKAAGFAVLIRFLKITFAQGLDKSGYWQMLSYIDWQTLLILFSII
                     TMTFGNFAALWQDNIKRMLAYSSIAHAGYLMLGVVVLSDQGLMAVLIYFSVYLFMNLG
                     AFYVVMLIANKINSEEIDDYRGLGYSLPLLGTALGIFLVSLTGLPPTAGFIGKLYIFI
                     ALVDANMITVAIIALLNTVVSLYYYIRVLKAMFLVKSEKHVEVNLTLLNYIVLALLIA
                     PVLILGVYFTPLVNIAKESIQLLGF"
     gene            973..1380
                     /locus_tag="VF870_15585"
     CDS             973..1380
                     /locus_tag="VF870_15585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9253667.1"
                     /translation="MLKLSKKSEYALMAARYMAIKNHGNAVTAKEIAENYKISFELVA
                     KVLQNLTKNNIAISFQGVKGGYTLKKNPLEISLIDLIQAVEPDYQITECLRSEAPKND
                     CSYLDCCKIRDPLAEVQKKIDKVFYETKLAHII"
     gene            1380..2579
                     /locus_tag="VF870_15590"
     CDS             1380..2579
                     /locus_tag="VF870_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX9253668.1"
                     /translation="MNIKTPIYLDNNSTTPIDPRVLEAMLPYFQDRFGNSSSNHSFGW
                     FAKSAVENARDILANFLNVSAKELFFTSGATESINIVHLGLAELANPDSFHIITSNLE
                     HSASTESLNHLANKGFEVTFLNVDKSGIIDPSKIADAIKFNTKLISIIFANNEIGSIN
                     DIKAIGQICKKHNIFFHVDATQAVGKLKFDSKELNIDFLSFTAHKLYGPKGIGALYIN
                     SKNPGIKLSPILFGGAQEGNIRPGTLNVPSIVGFGKAIELCEEEMTRNFDHTKQLRDR
                     FYKNIISNLDGVCVNGSMKERLPNNINFYVEGVRADKLMLELRELAFSNSSACSSGSA
                     KPSKILKAIGLTDEQALCSVRFGFGRFNTVDEIDYASQKVIEAVNKIIAIKKNKSYNY
                     TQINEKP"
     gene            2576..2887
                     /locus_tag="VF870_15595"
     CDS             2576..2887
                     /locus_tag="VF870_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253669.1"
                     /translation="MSNNISKLFDDLKNESKIFLSYLKAKFPVFHNSNLFSRDFQFGL
                     KSYLEKKGFKITDNNLVSLSKQFSGYYENKGILIKTSDQGWKINYPEFVTTKPGDPFS
                     F"
BASE COUNT         1023 a          479 c          512 g         1024 t
ORIGIN      
        1 aataaattca tttcttcaat tccctgctgc cagccagtta acctttttat tggcaatatt
       61 aatgatcttt gcaggaattg ggtttaagat atcgatcgtt ccgttccatt tctggactcc
      121 cgacgtttat gaaggcgcac ctatctcaat tacagcattt ctatccgttg caagtaaagc
      181 cgccggattt gcagtgctca tcagatttct aaaaattaca ttcgctcaag gacttgataa
      241 atctggttac tggcaaatgt tatcctatat cgattggcag acattgttga ttttattctc
      301 gattatcaca atgacttttg gaaattttgc tgcgctatgg caagataata ttaagagaat
      361 gctagcctat tcaagtattg ctcacgccgg ttatttaatg cttggggttg ttgttctttc
      421 tgatcaggga ttgatggctg tgttaattta cttttcggtt tatctattta tgaaccttgg
      481 tgctttttat gttgtgatgt taattgcaaa taaaataaac tcagaagaaa ttgatgatta
      541 cagaggctta ggttactcgc ttcctttact aggaactgca ctcggtattt ttctagtttc
      601 tttaacagga cttcctccaa ccgccggctt tattggaaaa ctttacatct ttattgcatt
      661 ggtagatgct aatatgatca cagttgccat aattgctcta ttaaataccg ttgtttctct
      721 ttactactac ataagagttc ttaaagctat gtttttggtt aaatcagaaa aacatgttga
      781 agttaatctt actttattaa actacatcgt acttgcactg cttatagctc cggttttgat
      841 tcttggtgtt tactttactc cactcgtaaa catcgctaaa gaatcaattc aactattggg
      901 attttgatca catcaatttg caattaaact caatttctaa taagtttata cccgactata
      961 atagtcttat ttatgttaaa actatcaaaa aaatctgaat acgcacttat ggcagcacgt
     1021 tatatggcta taaaaaacca tggtaatgct gttacagcta aagaaatcgc tgaaaattac
     1081 aagatttctt ttgaattggt agccaaagtg ttgcagaatc ttacaaaaaa taacattgca
     1141 atttctttcc agggagttaa aggcggctat acgttaaaga aaaatcctct ggaaatatca
     1201 ttgatagatt taatacaagc tgttgaacca gattatcaaa ttacagaatg tttgagatcc
     1261 gaagcaccaa aaaatgattg ttcatatttg gattgctgta aaatacgtga tccattagca
     1321 gaagtacaga aaaagattga taaagtattt tatgaaacaa agttagcgca tattatctga
     1381 tgaatataaa aacaccaata tatctggata ataactcaac aactccgatt gatccaagag
     1441 ttttggaggc tatgctcccc tattttcagg atcggttcgg gaactcttca agtaatcaca
     1501 gtttcggctg gtttgctaaa agtgcagttg aaaatgcaag agatatatta gctaactttc
     1561 ttaatgtatc cgccaaagaa ttatttttta ccagtggtgc tacagaatca attaatattg
     1621 tgcaccttgg gttagcagaa cttgctaatc cggattcttt tcatatcatt acatcaaatc
     1681 ttgagcacag tgcttctaca gaatctttaa atcatttagc gaataaagga tttgaagtta
     1741 cttttttaaa tgtagataaa tcaggaataa tagatccatc taaaattgct gatgcaatta
     1801 aatttaacac taaacttatt tctatcattt ttgcaaataa tgagatagga tctataaatg
     1861 atattaaggc aattggccaa atatgcaaaa aacataatat tttttttcat gttgatgcga
     1921 cccaggcagt tggaaagcta aagttcgact caaaagaatt aaatattgat ttcctatctt
     1981 tcacagctca taaactttac ggacctaaag ggattggtgc tttatacatc aatagtaaaa
     2041 atcctgggat taaactttcg ccaattttat ttggcggagc tcaggaaggc aatataagac
     2101 ccggaacttt aaatgtccct tccattgttg gatttggaaa agctattgaa ctttgcgaag
     2161 aagagatgac aagaaatttc gatcatacaa aacaattaag agataggttt tacaaaaaca
     2221 taatatcaaa tcttgatggt gtttgtgtaa atggttcaat gaaagaaaga cttccgaaca
     2281 acataaattt ttatgttgaa ggagttcggg ccgataaact aatgctcgaa ttaagagaat
     2341 tagcattctc aaattcatct gcatgctctt caggttcagc taaaccgagc aaaattctta
     2401 aagcaattgg gttaacagat gagcaggctt tatgttcagt cagatttggt tttggtagat
     2461 tcaacacagt tgatgaaatt gactatgctt cccaaaaagt aattgaagcg gtaaacaaaa
     2521 ttatagctat taaaaaaaat aaatcatata actatacaca aataaatgaa aaaccatgag
     2581 caataatatt tcaaaacttt ttgatgatct aaaaaatgag agcaagattt ttttaagtta
     2641 cctaaaagca aagtttccgg tattccataa ttcaaactta ttctccagag attttcagtt
     2701 tggattgaaa agctaccttg agaaaaaagg ttttaaaatt acagataata atctagttag
     2761 tctctcaaag caattttctg gttattatga aaataaaggt atacttatta agactagcga
     2821 tcaagggtgg aaaataaatt atccggaatt tgttacaaca aaacccggcg atcctttttc
     2881 attttagaat ataaaataat ttttgttttg cgggtttagt gatcaagttt gtaattttta
     2941 aaagttattt aggaggcggt tactactgtt tcaactgagg aatactttag tttttacatt
     3001 agagcttaaa aatttggatt acaataaaaa taagttaa
//
LOCUS       DASWBY010000864         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_562495, whole genome shotgun sequence.
ACCESSION   DASWBY010000864 DASWBY010000000
VERSION     DASWBY010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562495"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VF870_15600"
     CDS             complement(<1..809)
                     /locus_tag="VF870_15600"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9253670.1"
                     /translation="MKEKVILQTNFPKLNFVKSGKVRDIYDLGEYFLIISTDRLSAFD
                     VIMGQGIPYKGKVLTKISEFWFDYSKDIIKNHLITTDVNKFPKECLEYAEVLDGRSML
                     VQKADVVQIESVVRGYITGSGWVDYKKTGEVCGIKLPTGLVESEKFPEPLFTPATKAE
                     IGLHDENISEEKAKEIAGVETIDFMKNKTIEIYKKAVEFALSKGIIIADTKMEFGKIG
                     NEIILVDELLTPDSSRFWPLDKYEKGKSQESFDKQFVRDYLISIKFNKQPPP"
     gene            complement(923..>1594)
                     /gene="amrB"
                     /locus_tag="VF870_15605"
     CDS             complement(923..>1594)
                     /gene="amrB"
                     /locus_tag="VF870_15605"
                     /inference="COORDINATES: protein motif:HMM:NF013992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX9253671.1"
                     /translation="EFIKSKLPKDDFIVSNKAHSIEHSIEALIPFLQFYNRDIKITPI
                     MVTQMTMERMEDVSNRLSDTINEYAKSKNLKLGEDIFFLISNDANHYGEDFSNSPYRL
                     DANAHKLATENDVRIINQDLVNKISNEKIYQAAKDILPDSSNKFTPLWCGRYPIIFGL
                     MTISKVVKVFDNNELYGKLFKYSDTFSEKVLPVKNTSMGLTAVFSYKHWCGWFTEGFY
                     LTKNN"
BASE COUNT          507 a          288 c          226 g          573 t
ORIGIN      
        1 ggtggaggct gcttgttaaa cttgattgag attaaataat cacgaacaaa ttgtttatca
       61 aagctttcct gtgattttcc tttttcatat ttatccaaag gccagaatct tgaagaatca
      121 ggtgttaata attcatcaac cagaattatt tcattaccaa ttttgccaaa ttccatttta
      181 gtatcagcaa ttataattcc tttgcttaaa gcaaactcga cggccttttt ataaatttct
      241 atggtcttgt ttttcataaa atcaatcgtt tcaacaccgg caatctcttt tgctttttct
      301 tctgaaatgt tttcatcgtg caaacctatt tcggcttttg tagcaggagt aaaaagtggt
      361 tccggaaatt tttccgactc aactaatcct gtaggaagct taattccaca aacttccccc
      421 gtttttttgt aatcaaccca tccagatccg gttatatatc cacggacaac gctttcaatc
      481 tgaactacat cagctttttg aacaagcata cttctgccat ctaaaacttc agcatactcc
      541 aggcactctt tgggaaattt atttacatct gttgtaataa gatgattttt aattatgtct
      601 tttgagtaat caaaccagaa ctcggaaatt tttgttagta ctttcccttt atacggaatt
      661 ccctgtccca ttattacatc aaaagcagat agcctgtcag tagagataat taaaaaatac
      721 tcaccaaggt cgtaaatatc tcttacttta ccgcttttta caaaattcaa tttaggaaaa
      781 tttgtctgaa ggataacttt ttctttcatg ctaaacctat attattatgg ttcaaatata
      841 tgcaggctta aagcggaatt caataaaaag taaaattgaa aattgaggat taaaatttaa
      901 tttgtcttaa aaatccacta attcagttgt ttttggttaa ataaaatccc tcagtaaacc
      961 agccgcacca atgtttgtaa gaaaatacag cggtcaaacc catgctggta tttttaactg
     1021 gtaaaacttt ttctgaaaaa gtatctgagt atttaaataa tttcccgtat agttcgttat
     1081 tatcaaatac tttaacaact tttgaaattg tcattaatcc aaaaatgatt ggataacgcc
     1141 cgcaccagag aggagtgaat ttatttgatg aatccggtaa aatgtctttt gccgcctgat
     1201 aaatcttttc attggatatt ttatttacta aatcctgatt tatgattcgc acatcatttt
     1261 ctgttgcaag cttgtgagca tttgcatcca atctgtaagg cgaattacta aaatcctcac
     1321 cataatgatt cgcatcattt gaaatcaaga aaaaaatatc ttcacccaat ttcaggtttt
     1381 tcgattttgc atattcattt attgtatcag ataatcgatt cgatacatct tccattcttt
     1441 ccatagtcat ttgagtaacc atgattggag tgattttaat atcacgatta taaaattgta
     1501 aaaatggtat aagcgcttcg atagaatgct caatgctatg cgccttattg ctaacaataa
     1561 aatcatcctt tggaagtttt gatttaataa attc
//
LOCUS       DASWBY010000865         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_562510, whole genome shotgun sequence.
ACCESSION   DASWBY010000865 DASWBY010000000
VERSION     DASWBY010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562510"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..410
                     /locus_tag="VF870_15610"
     CDS             105..410
                     /locus_tag="VF870_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253672.1"
                     /translation="MDPNEIFNNALSSKYIIIDENELKTILDNCEIVRVEDTHLSDFI
                     RVLNYENKLFVQETTFKQEILIRKMDSMKDVDSFVQERLDYYERKWDGCGCKIDYYE"
     gene            complement(554..2164)
                     /locus_tag="VF870_15615"
     CDS             complement(554..2164)
                     /locus_tag="VF870_15615"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX9253673.1"
                     /translation="MSIIKEIKNRKTFAIISHPDAGKTTLTEKLLLFSGAIQIAGAVK
                     SNKIRKTATSDFQEIEKQRGISVSTSVLSFKYKGVNINLLDTPGHKDFAEDTYRTLTA
                     VDSVILVIDCVKGVEEQTEKLMNVCRMRNTPVIVFINKLDREGRNPIELLDEIESKLN
                     IKVRPLSYTMGIGVSFRGVYNLYENSFSFFKANKQRIEDEIEKFNGVDDPKLIEYFGD
                     DALKLKEDIELIHGAYENFNIEDYLNGTTAPVFFGSAVNNFGIKELLDTFIKIAPQPL
                     PRETTMGQIKPDRDEFSGFIFKIHANLDPKHRNRIAFLRVCSGKFERNKFYHHVRLNR
                     DIKFPNPASFMAQDKTTIDEAFPGDVIGLYDVGNFKIGDTITEGENFMYKGIPTFSPE
                     IFKELHLEDPFKSKQLNKGILQLTDEGLAQVFIQNNGSRKVVGTVGDLQFDVLEYRLL
                     NEYGAACRFRPLNFYKACWVVYEKEEDISELMRIRSHNLFLDKHNKLVFLAESQYSIA
                     SAQEKNPKAKFLFSVEHNTQTHELENLE"
BASE COUNT          753 a          418 c          356 g          834 t
ORIGIN      
        1 tatctgattg cgagtgcccc gtaattgttg ttaactcttt ctaaaattac actgaatcct
       61 cgcctccctt tgtttatcta atccagtaaa aaaagaaaaa tcttatggat ccaaatgaaa
      121 tatttaataa tgcacttagc tcaaaatata ttatcataga tgagaatgag ctaaaaacta
      181 ttttagataa ttgtgaaatt gtaagggttg aagacacaca tctttcagat ttcattcgtg
      241 ttttaaatta tgaaaacaaa ctatttgtgc aggaaacaac ttttaaacaa gaaatattaa
      301 ttcgtaagat ggattcgatg aaagatgttg actcctttgt gcaggaaaga ttggattact
      361 atgaacgcaa atgggatgga tgtggatgta agattgatta ttatgagtga caattttgta
      421 ttatcagttt aactgtataa attttttgtc attcgaccag ggggagaaat ctgaacttta
      481 tattttaaca gatttctcag tcgcagactc cttcgaaatg actttaataa tgtgcttcaa
      541 tttcactaac ttttcattcc aaattctcaa gttcatgtgt ttgagtatta tgctcaacac
      601 tgaataaaaa tttagcttta ggattttttt cctgcgcact ggcaattgag tactgtgatt
      661 ccgccaagaa aacaagttta ttgtgtttat ctaaaaataa attatgtgaa cggattctca
      721 ttaactcact tatatcttct tccttttcat aaactaccca gcaagcttta taaaaattca
      781 gcggacgaaa tctgcatgcc gcaccatatt cattgagtaa tctatattca agcacatcaa
      841 attgaagatc tccgactgtt ccaacaactt ttctgcttcc gttattctgg ataaacactt
      901 gagcaagtcc ttcatcagta agctgcaata ttcccttatt aagctgtttg gatttaaatg
      961 gatcttcgag atgaagctct ttaaatattt ccggcgaaaa agtcggaata cctttataca
     1021 taaaattttc accttctgta attgtatctc cgattttaaa atttccaaca tcataaagac
     1081 cgattacatc gccgggaaat gcttcatcaa tcgttgtttt atcctgtgcc ataaaagatg
     1141 caggatttgg aaacttaata tcacgattta gtcttacgtg atgataaaac ttatttctct
     1201 caaactttcc ggaacataca cgaaggaatg caattctgtt acgatgcttt ggatccaggt
     1261 ttgcgtgaat cttaaaaata aaaccggaaa attcatctct atccggttta atttgtccca
     1321 ttgttgtttc tctgggcagc ggctggggtg caatctttat aaaagtatcc agcaattctt
     1381 taattccaaa attgtttact gcacttccaa agaataccgg agcagttgtg ccattcaaat
     1441 aatcttcgat attgaagttt tcataggcac catgaataag ttctatatct tctttaagct
     1501 ttaatgcatc atcaccaaag tattcaatta attttggatc atccactcca ttaaactttt
     1561 caatttcgtc ttcaatcctc tgcttgtttg ctttaaagaa tgaaaatgag ttttcataaa
     1621 gattataaac acctctaaag ctaacaccaa ttcccatagt ataacttaac ggacgaactt
     1681 taatgtttag cttgctttca atttcatcga gcagctcaat tggatttctt ccttcgcgat
     1741 caagtttatt tataaaaact ataacgggag tgttacgcat tctgcaaaca ttcataagtt
     1801 tttctgtctg ttcttcaact cctttaacgc aatcgataac aagaataaca ctatcaacag
     1861 cagttaatgt tctataggta tcctcagcaa agtctttatg cccgggagta tcaagcagat
     1921 ttatatttac ccccttgtat ttaaaactta atacagatgt agaaacggat attcctctct
     1981 gcttttcaat ttcctgaaaa tctgaagttg ccgtttttct tatcttattt gatttaacag
     2041 cacctgcaat ctgaatagca ccgctaaaca atagtaattt ttcagtcaat gttgtttttc
     2101 cggcatcagg gtgactaata attgcaaatg ttttccggtt ctttatttct ttaataatac
     2161 tcattataat tttcttttaa tttcaaatta gtttggataa ataaaacagt cggcttgtta
     2221 aaaagcctaa atagagttga aatgcgagct aataattttt attcttaaac atgctggtgc
     2281 aaatataaga aaaatcagtc aaactaaatt taacttgtag atgataatta tatcgtagtg
     2341 attggttcct taaaatattt t
//
LOCUS       DASWBY010000866         5348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_600016, whole genome shotgun sequence.
ACCESSION   DASWBY010000866 DASWBY010000000
VERSION     DASWBY010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600016"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VF870_15620"
     CDS             complement(<1..672)
                     /locus_tag="VF870_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9253674.1"
                     /translation="MKKKSTNYPRLLEINARIWIKRFGDECTLGSIPDEQINKWKELG
                     IDMIWLMGVWDNNKSVINEYCFEPELISSYNGALKDWRNEDVIGSPYSIDKYELNPLL
                     GTRKDLLSFKKKLNDAGIKLILDFVCNHFSAKSSLIWTNKEIFLPADEFIFKNDPYTF
                     FQSPANNKEYLAHGRDPLFPPWKDTAQINFYSREARNYLTSVLIELTELCDGVRCDMA
                     MLPLNN"
     gene            1118..2101
                     /locus_tag="VF870_15625"
     CDS             1118..2101
                     /locus_tag="VF870_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253675.1"
                     /translation="MSITTIKEKDNTTKNLLNPDVANNELELELEEYTRSAVRNIQFT
                     VILKPAIISFIALFISFFLDLSSVPILGNVTTDLAKSLFPSWQPVKESVIPFSFWWLP
                     VVTYALFIFLAFLAYSKLKLEIIRTPASATIDRVIGSYNSVIDSIATALPLIGAAILL
                     ISIKLGEEIFLGLSVPFEVKALIVLAIGKLFEPVLDQLGMEFQAVVNHVSDIRDKYFS
                     RIQVENSKNILKQLNQGGSGVGGTELSLADVEKFKMLVEQSAKLSEIMNRNFTSISAL
                     ADKVNQIEGVSSQKIIELNGLANALNQASQSLGDEKTLTGLKHLEAIVLKK"
     gene            2114..3061
                     /locus_tag="VF870_15630"
     CDS             2114..3061
                     /locus_tag="VF870_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253676.1"
                     /translation="MINKNRDSKFVIYQVLYIFVITVLALKGADLDLRRVALEENTVQ
                     KSVRDSLIAVLDSLYSLGIDFSIKIDPNVVVENEQMREQLAALNKKLETLKDYVPPPT
                     EKEPKEEKVEEQVLLQSPISAKQTYIQYTWNIARNTGSVPANIYDPRNMNSPIVVVPP
                     GQEKKFDLTDQTEVIVKYGSQEDRIKVLPNKPPEIKIERVTTKMNSADIYVKDLQRIT
                     AFKVTIVDQRPDQLKVTYTGPISVSGPTKDSNGNLIYNVSLKIASTENAFDSWLDKNG
                     NLREADGRYKVNFFFTAVDEISKDRVQVGDSFFFTEFSN"
     gene            3082..4254
                     /locus_tag="VF870_15635"
     CDS             3082..4254
                     /locus_tag="VF870_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253677.1"
                     /translation="MKASKLIFVFLTLVLILGFNSIYAQEEEEQIRYYQMEPLDDSLF
                     INIQVALFIDPPDPKAEIVVDLRDANNQTISIKSVLYPFLAVPPELRARIITYPFKLN
                     LEEDINYGSVFTRVLQKIRVSKVAQPPTKMQISPTLGYINPFFQLQGGERLGLSLKQD
                     VGLSMGVGTPYSGPLETNMVEANFHILGVRAGAFSHVDAMIEIIKDQNHNNLIVSEGF
                     QINYVIPFGNFFEFGYFKATGKIGESKKNAYISENEGSIALNEDSTIKYQARIIDNDQ
                     SYLNWELRYPVSILGSTRGRVYVSQYLDEWHFGFALREASLAGSTFDVSFDGMPGSKY
                     RQPQYNINFMVQKIMEGWGFSAFSIGPSASISRLDTGKIGFTKIFVNIRFKIGTSL"
     gene            4364..5125
                     /locus_tag="VF870_15640"
     CDS             4364..5125
                     /locus_tag="VF870_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253678.1"
                     /translation="MKKNFIILWLASFVIVFVTVYISNILDKDYPITSTFGIEGKKVS
                     YRFKKVHYGKEDYKVIIRTDIDELTGKLLWKSDTDINWRSKELEKSKLILSGTIPVLK
                     PENKIDYYLELNYKDKKYVLPTNKKVSLTFFGKIPVVVNVLEYLLLYLGLILAVRTGL
                     EFFNNGLKSKKFGFFTLALFLTLIALINPLYLTYKYGFINSSIPPISRLFLLSDILIF
                     ILWIATLVTIFRSEKFKFLPLISAVITLIIFVLFK"
     gene            complement(5135..>5348)
                     /locus_tag="VF870_15645"
     CDS             complement(5135..>5348)
                     /locus_tag="VF870_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9253679.1"
                     /translation="DCYGYYLLATGYADIMIDPIMSVWDSMALIPIINGAGGVITDYQ
                     GKDTTLGNSIVASNKVIHSEVIKLLN"
BASE COUNT         1866 a          844 c          898 g         1740 t
ORIGIN      
        1 attatttaac ggaagcattg ccatatcgca tctcactcca tcacataatt cggtaagttc
       61 aatcaaaacc gaagtaagat aattacgcgc ttctctgctg taaaaattta tctgcgcagt
      121 atctttccag ggcggaaaca atgggtcgcg tccatgcgca agatattctt tattgttagc
      181 tggtgactga aaaaatgtat agggatcatt tttaaaaata aattcatctg caggtaaaaa
      241 tatttctttg ttagtccaaa tgagtgaaga ttttgcactg aagtgattgc acacaaaatc
      301 aagtataagt ttgattcctg catcgtttaa ttttttctta aatgataata aatctttcct
      361 tgttccaagt aaggggttta attcatactt atcaatagaa tatggtgaac caattacatc
      421 ttcattacgc cagtctttta atgcaccgtt ataggaagaa ataagttcgg gttcaaaaca
      481 atattcgttt ataactgatt tgttattatc ccaaactccc atcaaccaga tcatatcaat
      541 accaagttct ttccacttgt ttatctgctc atctgggata gatcctaatg tacattcatc
      601 tccaaatcgt tttatccaaa tgcgggcatt tatctcaagc aatcttgggt aattagtgct
      661 tttctttttc attcctgaag attagtttaa aacaaaatat taatctttaa gtaaagataa
      721 atataatatt aattgcattt gattataaaa tagaatgagg atgagattaa tatttcaggt
      781 aataattaca gaattgtaaa gaatttttaa ccaataggta aatggttcat aacttaattt
      841 gaaactaaaa ttaatatgtg taaataattg tctggtaatt caatattttt aagcttaaaa
      901 ttataatgtt atattaaata gcttaatatt tgattgaaaa ccaagtattt tttctacaaa
      961 aatagatttt cgattacaag cataattccc aaacataaaa acaatataag gaacagtgtt
     1021 atgataattg tttcaaacaa gaaccgtttt ggttagttaa acataactat gtgcattttt
     1081 tacataaaag ttttaccaag aaaatttgag gagactgatg tcaattacaa caataaaaga
     1141 aaaagataat acaactaaaa atcttttaaa tcccgatgta gctaacaatg aactagaact
     1201 tgaacttgaa gaatatacta ggtctgcggt aagaaacatt caatttacag taatattaaa
     1261 gcccgccatt atctcattta ttgcactgtt tatttccttt tttcttgatc tttcaagtgt
     1321 gccgatatta ggtaacgtaa ccactgatct ggcaaaatct cttttcccga gctggcagcc
     1381 agttaaggaa tcggttattc catttagctt ctggtggttg cctgttgtta cttatgctct
     1441 gtttatcttt cttgcttttc tagcatacag caaattaaag ctcgaaatta tcagaacacc
     1501 ggcaagcgcc acaatagata gagtaattgg atcttataac agtgtaattg atagtattgc
     1561 aactgcatta cctttaatcg gtgctgcaat attacttata agcattaaac ttggagaaga
     1621 aatctttctt ggcttgtctg taccgtttga ggtaaaagct ttaattgtgc ttgcaatagg
     1681 aaaattattt gaaccggtac tcgatcaact tggaatggaa tttcaagccg tagttaatca
     1741 cgtttctgat ataagagata aatatttttc aagaatacaa gtagaaaatt caaaaaacat
     1801 tttgaagcag cttaatcaag gcggctcagg tgtaggtgga actgaattat cacttgcaga
     1861 tgtcgaaaag ttcaagatgc ttgtagaaca atctgcaaag cttagcgaaa ttatgaacag
     1921 gaattttaca tcaataagtg cacttgcaga taaagttaat cagattgaag gagtaagttc
     1981 acaaaaaatt attgagctta atggattagc aaatgcatta aatcaggctt cacaatcact
     2041 gggtgatgaa aaaacgttaa ccggattaaa acacctggaa gcaatagtat taaagaaata
     2101 aaaagatttt aaaatgataa ataaaaacag agatagtaaa tttgttattt accaggtact
     2161 gtacattttt gttattacag tgcttgcgct taaaggagct gatcttgatt tgcggagagt
     2221 tgcacttgaa gaaaatactg tacaaaaatc tgttagagat tcactgattg cagtattaga
     2281 ttcgctttat tcactcggta tagacttttc aatcaagatt gatcccaatg tagtggttga
     2341 aaatgaacaa atgagggaac aattagcagc tctaaacaaa aaattagaaa ctctaaaaga
     2401 ttatgttccc cctccaacag aaaaagaacc aaaggaagaa aaggttgaag aacaagttct
     2461 tcttcagtca ccaatttctg caaagcaaac atatattcag tatacctgga acatcgcaag
     2521 aaatacagga tcagtccctg caaatattta tgatccaaga aacatgaata gcccaattgt
     2581 tgttgtaccg ccgggccagg aaaagaaatt tgatcttaca gatcaaacag aggtaatagt
     2641 aaagtacgga tcgcaggaag acagaataaa ggttttgcct aacaaaccgc cggaaattaa
     2701 aattgaacgt gttacaacaa aaatgaattc cgctgatatc tacgtaaaag atttacagcg
     2761 tattacagct tttaaagtta caattgttga tcaacgacct gatcagctta aagttactta
     2821 cacaggacca atatccgtaa gtggaccaac caaggacagt aacggaaatt taatttataa
     2881 cgtatcatta aaaattgcat caacagaaaa tgcattcgat agctggcttg ataaaaacgg
     2941 taatcttaga gaagcagatg gaagatataa agttaacttc ttctttaccg ctgtcgatga
     3001 aatatcaaaa gacagagtac aggttgggga ctctttcttc tttacagagt tttctaatta
     3061 attattcagg catatataat aatgaaagca tctaaactaa tttttgtatt cctaactctt
     3121 gtactgattc tcggatttaa ttctatctat gcacaggaag aagaagaaca aatcaggtat
     3181 tatcaaatgg aaccattgga tgatagtttg ttcatcaaca ttcaggtagc tcttttcata
     3241 gatccccctg atcctaaagc agagatagta gttgatttga gagatgctaa caatcaaact
     3301 atttcaataa aaagcgttct gtatccattc ctggctgtac ctccggaatt aagagcaaga
     3361 attatcacat atccatttaa attaaatctt gaggaagata ttaattatgg aagtgttttt
     3421 accagggtat tacaaaaaat acgggtaagt aaagtggctc agcctccgac aaaaatgcag
     3481 atatcaccta cacttggtta tataaaccct ttctttcagc tgcagggcgg tgaaagatta
     3541 ggtctatcat taaagcagga tgttggtcta tctatgggtg ttggaacccc ttattcaggt
     3601 ccccttgaaa cgaatatggt tgaagctaat tttcatatac tcggggtcag ggctggtgcc
     3661 tttagccacg ttgatgcgat gatagaaata attaaagatc aaaatcataa taatctgatt
     3721 gttagtgaag gatttcagat aaactatgtt attccgtttg gtaacttttt tgaatttgga
     3781 tacttcaaag caaccggtaa aataggtgaa agtaagaaaa acgcttacat tagtgaaaat
     3841 gaaggctcaa ttgccttgaa tgaagattca accataaaat atcaagctag aattattgat
     3901 aatgatcaat cctatcttaa ttgggaattg cgctatcctg tttcaatact tggttcaacc
     3961 cgagggaggg tttatgtgtc ccagtattta gatgaatggc attttggttt tgcactaaga
     4021 gaagcatcac ttgccggaag tacttttgat gtaagttttg acggaatgcc gggtagtaaa
     4081 tatcgccaac ctcaatacaa tataaatttc atggttcaga agataatgga aggatggggt
     4141 tttagtgctt tttcgattgg cccatcagcc agtatttcca gacttgacac gggtaaaatt
     4201 ggatttacaa aaatatttgt aaacatcaga tttaaaatcg gtacttctct ttaataaaat
     4261 tattatttat aagccccggt taagctgggg cttttttatt ttttatcttt ccgcctgaat
     4321 aaaatctact taaatttgtt tataacatta atacccgata gaaatgaaaa agaattttat
     4381 aatactttgg ttagcttctt ttgtaattgt atttgttact gtttacatct caaatattct
     4441 cgataaagat tatcctataa cttctacctt tggaatagaa ggcaaaaaag tttcttatag
     4501 atttaaaaaa gtgcattacg gcaaagaaga ttataaggtt ataatacgaa cggatattga
     4561 tgaacttacc ggaaaattac tttggaaaag tgataccgat atcaactggc gatcgaaaga
     4621 attagaaaaa tctaaattga ttttatcagg taccatcccg gttttaaagc ctgaaaataa
     4681 aatcgattat tatctggaat taaattacaa agataaaaaa tatgttctgc cgactaataa
     4741 aaaagtcagc ctaacattct ttggaaaaat tccggtagtt gtaaatgtac ttgaatattt
     4801 actgctttat ttggggttga ttttagcggt cagaacagga ttagaatttt ttaataatgg
     4861 attaaaatcc aaaaaatttg gtttctttac cttggcttta ttcttaacac ttattgcatt
     4921 aataaatcca ttatatctta cttataaata tggatttata aattcctcta tacccccaat
     4981 cagcagactc tttttattaa gcgatatttt gatatttatt ttatggattg ctacgcttgt
     5041 tacaatattc agatctgaaa aatttaagtt tttaccgttg atatcagcag taataacttt
     5101 aataattttt gttcttttca agtaagcaca atcattaatt taaaagtttt ataacttcag
     5161 aatgaattac tttatttgat gcaacaatgc tatttcctaa agttgtatct ttaccctgat
     5221 aatctgttat cactccgccc gctccattga taattggaat taatgccatc gaatcccaaa
     5281 cactcataat cggatcaatc ataatatctg catatccggt agcaagcaaa tagtagccgt
     5341 aacaatcc
//
LOCUS       DASWBY010000867         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_629643, whole genome shotgun sequence.
ACCESSION   DASWBY010000867 DASWBY010000000
VERSION     DASWBY010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629643"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VF870_15650"
     CDS             <1..248
                     /locus_tag="VF870_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253680.1"
                     /translation="IGGVKIHQFRKIKENRVTKKIVITKNGNLSQFEESVRMFTKDEL
                     VKAIQNIGFDIYKTFGDFLGNDFEQFSSPRLILICKK"
     gene            245..826
                     /locus_tag="VF870_15655"
     CDS             245..826
                     /locus_tag="VF870_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253681.1"
                     /translation="MNKIIFITLITISFTACDLFNTRNAETPDQTRSNWQPPVEPAIV
                     IENLKNSLSDKNVQNYIACFVDTIFAHQNYNFSASSEATSRYDILQNWGLNEERRYFT
                     SITNKVPADFPISLTLSNQNFSNLSGDSLIYSATYFLNLPLSSGDPFPQNYAGSLQFN
                     MLRDSRSEWVIYFWKDTKSETLPSWSELKGSSY"
     gene            849..1325
                     /locus_tag="VF870_15660"
     CDS             849..1325
                     /locus_tag="VF870_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253682.1"
                     /translation="MISFIGCTNPFAPGLDENNDNFKPPISDQKNIDGVFQNFQYAYT
                     FKDSTIYGGLITNDFTFTYRDYDQGFDVTWGRDEEMRTTYGLFQNTQRLDLIWNNIVL
                     ITEDSLSANIVRSFNLTIIFNPTDVVRVDGKVNLTMNKNTDSGVWKINKWIDESNF"
     gene            1350..2213
                     /locus_tag="VF870_15665"
     CDS             1350..2213
                     /locus_tag="VF870_15665"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX9253683.1"
                     /translation="MILFWIKEAFKLIARSKFSFLLALTSITLSVILITISVFIIRFS
                     NHFEDQLKSNIVISVFIKDKITNDEIDQIKTELHQLKYLSSSEFIDKDKAAEIFIKET
                     GEDFRKILDYNPLPASFNLKLKNEYANRDSIKKIINTLSGYNWSDEVIFRQDFYQKIL
                     FYIDQAKVYVFALTGLIFLVSLYLVYSTVRLILNSKYSELETMKFVGAKLSTIKMPII
                     LNSAITGLLSGLIAFGLIWALYHYIKELLITIDAVIPNKVEFIVLIMSLGPIIGILVT
                     ILSLRKISLKI"
BASE COUNT          788 a          336 c          368 g          803 t
ORIGIN      
        1 atattggtgg agttaaaatt catcagttca gaaagatcaa agaaaacaga gtaacaaaaa
       61 aaatagttat tactaaaaat ggcaatctta gtcagtttga agaatcggtt agaatgttca
      121 ccaaagatga gttagttaag gctatccaaa atattggatt tgatatttat aaaacatttg
      181 gtgattttct tggtaatgat tttgagcaat ttagttcacc acgattgatt ttgatttgta
      241 aaaaatgaat aaaataattt ttattacctt aattaccatc tcttttacag cttgtgatct
      301 tttcaataca cgtaatgcag agaccccaga tcaaacaaga tcaaactggc agcccccggt
      361 tgaacctgcc attgttattg agaacttaaa aaactctcta tcagataaaa atgttcagaa
      421 ctacattgcc tgttttgtag atactatttt tgcgcatcaa aattataatt tttcagcatc
      481 aagtgaagct acctcacgtt acgatatcct tcaaaactgg ggtttgaatg aagaaagaag
      541 atattttaca agtattacta ataaagttcc tgctgatttt cctatttcat taacactgtc
      601 taaccagaac ttcagtaatc tcagcggtga ttcattaatt tattcagcaa catatttttt
      661 aaatcttcct ctatcctctg gtgacccatt cccgcagaac tatgcaggta gcttgcaatt
      721 taatatgctg cgcgattcac gttctgagtg ggttatttac ttttggaaag atactaaaag
      781 tgaaacatta ccaagttgga gtgaattaaa aggaagttca tattaaaatt ttatttagca
      841 tattgctatt gattagtttc atcggttgta caaatccttt tgcacccggg ctggatgaaa
      901 ataatgataa ctttaaaccg ccgatttcag atcaaaaaaa tatcgacgga gtatttcaga
      961 actttcaata tgcttatact tttaaggatt ctactattta tggtggattg attacaaacg
     1021 atttcacttt tacgtatcgt gattatgatc agggatttga tgttacctgg ggcagagatg
     1081 aagagatgcg caccacatat ggattattcc agaatacaca aagattggat cttatctgga
     1141 ataatatagt gctgattaca gaagattcgc tttcagccaa cattgttagg agctttaatc
     1201 ttacgattat attcaatcca acggatgttg taagagtgga tggaaaagtt aatttaacaa
     1261 tgaataaaaa tactgattcc ggcgtttgga aaattaataa gtggattgat gaatcaaatt
     1321 tctaaaaaca tttttagtaa aattacgata tgattttatt ctggataaaa gaagcattta
     1381 aattaattgc aagatcaaag ttttcatttt tgcttgcact tacttcaatt acactatcgg
     1441 taattttaat aactatatca gtttttataa ttcgtttttc aaaccatttt gaagatcaat
     1501 taaaaagtaa cattgttatt agtgttttta taaaagataa aattaccaac gatgagattg
     1561 accagattaa aactgaatta catcagctta aatatttaag ctcatctgag tttattgata
     1621 aagataaggc tgctgaaata tttatcaagg aaacaggtga agattttaga aaaattctgg
     1681 attataatcc attacctgca tcgtttaatc ttaaactaaa aaatgaatac gctaaccggg
     1741 actcaattaa aaaaatcatt aatactctat cgggttataa ttggtccgat gaggttattt
     1801 tcaggcagga tttttatcag aaaattttat tttatatcga tcaggcaaaa gtttatgttt
     1861 ttgctttaac aggattgatc tttttagttt cgctttacct ggtttatagt acggtaagat
     1921 taattcttaa ctcaaaatac tcagaactag aaactatgaa gtttgtgggt gcaaaattat
     1981 ctaccatcaa aatgccaatt attcttaaca gtgctattac cgggctgcta tcaggtctga
     2041 ttgcatttgg cctaatctgg gcactttatc actatataaa agaactttta atcacaatcg
     2101 atgcggtgat ccctaataag gttgaattta tagttttaat tatgtcgcta ggacccataa
     2161 taggaattct tgttacaata ctttctttaa gaaaaatttc gcttaagatt tgatttaatt
     2221 tatatttaag tataaagatt taaatctgat taattatcaa taacttatct aacaacattg
     2281 gttatagttt ggcta
//
LOCUS       DASWBY010000868         7587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_487417, whole genome shotgun sequence.
ACCESSION   DASWBY010000868 DASWBY010000000
VERSION     DASWBY010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487417"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /gene="feoB"
                     /locus_tag="VF870_15670"
     CDS             complement(<1..1280)
                     /gene="feoB"
                     /locus_tag="VF870_15670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX9253684.1"
                     /translation="MKTASAEKLHLITLVGPPNSGKTTLFNQLSGKNFKTVNYPGSTV
                     EYSTCKIQSKFGLDADVLDSPGIISLNPNSPDEEVSINSLYNHPKFGSPHLLIATADA
                     SQISRHLLLAKQLIESGFNVIVVLTMNDILAKRGYEIDENKLAEELGCGVIKVDGRTG
                     KGIDKLLSCVKPKLSTQDLESTRSLQQVFNNISKERLLSFYEDIERIEKNVIVPVKEI
                     TLPNLKKANEKLKILNPTLKYGSNRPDERTLKIDRFLLSRQWGLLFFFLIMAFTFTSI
                     FWLANPFMELVDEFFGFLSISAAGLLGHTWYSDLISDGIISGTGSVLVFLPQILILFL
                     ILGLLEDTGYLARGAMLIDKPLSKIGLNGKSFVPMLSGFACAIPAMLAARTISNKRER
                     LLTIFIIPLMSCSARLPVYALLIAFLTPADKAWLA"
     gene            complement(1277..1498)
                     /locus_tag="VF870_15675"
     CDS             complement(1277..1498)
                     /locus_tag="VF870_15675"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX9253685.1"
                     /translation="MKTASELNFGERAIISDIDNSHPSFRRLLEIGFTPGQEIELINS
                     SAFNDPLAFALRGTLIAIRKHEADCIILS"
     gene            2106..2639
                     /locus_tag="VF870_15680"
     CDS             2106..2639
                     /locus_tag="VF870_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253686.1"
                     /translation="MEIAKLAFLETYTLSENGGVMGAILVTDAETKPLEFRVTAPIRP
                     TSFQKTLYGDVLLEHILVELISVPLLNAVNEQIDLIVVKDPLFLGANNKQGIRVVRLL
                     NDEKEKSRSNTSVELLNTPLNGSEKGFVETSKKFEEELKGIKERLEKIAESRNLSEPF
                     ERLKAACEQVQLQRTND"
     gene            2655..5369
                     /locus_tag="VF870_15685"
     CDS             2655..5369
                     /locus_tag="VF870_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEX9253687.1"
                     /translation="MIRTAREKIATKVSKLEVADDFHFAKWLRPPKIHTADVTELYSI
                     DEVENFPVEQLTAPNSIKLKFRLAAYKIPEVRHSYLLDKPKAYLIFFGLTRPRLFTMK
                     EKILSAPEIFNIKSAIPQAPEIIDQSELFIAKAPELIDQSSKFIATPPASYDKSSQFF
                     FIQDPSYDLNFTFSNVKVHETPLLMSPPKITDELANLTPEIYNIELLNVEKFDHTLIN
                     SNDINIEKFPIPALIKIDVPGIDNLVESITESYAVPFEEMLQADVKFYSPSFEIAHPV
                     FKVKIELPRAIVKKVVIKQKNLEPIIIDALNDASVSEFAKNNIMSLLSSFRELSWQEY
                     SKMINPLTEYQLQSSEFLTSNNFAVLNDELGYEKFDQTSAALGYLAKKGNIKSVLLIS
                     DNIRYNDYWTPSLKAFVKDLKLKKVEPGITKKVKGTSVIWFLDVNDFGKIEIKDFDKF
                     DLVLFDELINIKSAADQVTQLITKIEPGYIWFLTAVINDKAVKKFLEEFDFTSKVTFA
                     AFGKSLADLQGEEPVTTIKNIWLELDEMQSFEYSEALNQSKEELNVLLESPNPLRFQS
                     NIFNIIHRLKQILNFSSFRNISPKANLLIEQVEAIANNKKKAMIFTQYDVNGMKKIEK
                     ALEMNNVKFVVGRNGMSAEELKQTLDGFYERRDVTVFLTNLKPSRININLTKVPYIFN
                     FDQWWNPITQWQNYDEMGISEIVHSPVVVYNYNIKNTFEEELSSLIKEKGLSNRYLFD
                     NLKSETLSELITMEDWLYIFGMNAQFNKMLNTERTKLVKKLQSINLNNYKALIKYFFS
                     FLGYRDISIMDIDDEPMFYIIGTARKGTTPVNLHGKCLLTTDVKKEDYEEVIHFKPSA
                     NEIKRKFVITNGEFAERIKNGTTYIDCKDLANFILTLGLKSHVNSKKN"
     gene            complement(5389..6327)
                     /locus_tag="VF870_15690"
     CDS             complement(5389..6327)
                     /locus_tag="VF870_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX9253688.1"
                     /translation="MQTAVITGGSGFLGSHLCDRLLKEGMNVVCIDNLLTGSLDNIQH
                     LFGNPKFKFIHHDVTNFIHVAGSVDYILHFASPASPLDYHLLPIQTLKVGSLGTHKAL
                     GLAKEKGARFLLASTSEVYGDPLMHPQKEDYWGNVNPIGPRGVYDEAKRFAEALTMAY
                     QRYHKLETRIVRIFNTFGSRMRLNDGRAIPAFFSQALNNQPITVFGDGSQTRSFIYID
                     DEVEGILRLLLSNEKEPVNIGNPEEITVKQMAEEIIELTGSKSKIIYEPLPVDDPQVR
                     QPDISKAKRVLGWEPKVQRSEGLKNTMEYFKLRIKG"
     gene            complement(6519..7220)
                     /locus_tag="VF870_15695"
     CDS             complement(6519..7220)
                     /locus_tag="VF870_15695"
                     /inference="COORDINATES: protein motif:HMM:NF045041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253689.1"
                     /translation="MKNYFSIIIIGLTLIINSPSINSQTAESTKSIKVTEEKSFGQEY
                     FPTEIKKTLIYDSSFGELELKVTKENNVKLFSYDSDKFKYRQKLFVNDKGLFVNETYQ
                     KIKLLLFITKEGNYVYDKPLLRIPFPVQVGQEWTWDGKEFVNDETHTVNIKGKATKFE
                     TITLPAGTFETLKVETTLQTSNGTKNVLTEWYAKDYGMVKMLVSIEGGGMLGFARDIL
                     GYGTIKFELKEVRDK"
     gene            7348..>7587
                     /locus_tag="VF870_15700"
     CDS             7348..>7587
                     /locus_tag="VF870_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9253690.1"
                     /translation="MNYLDKLEKVITQNCSIIAKELYLNSAVLIPIVILNDTEYVLFQ
                     KRSSTVRQPSEVSFPGGHYDSNCDKDFLSTAIRETC"
BASE COUNT         2558 a         1274 c         1217 g         2538 t
ORIGIN      
        1 gccagccatg ctttatcagc aggagttaaa aatgcaataa gcagggcata aacaggcagt
       61 cttgcactgc agctcataag cggaataata aatattgtca gtaaacgttc acgtttgtta
      121 gaaattgttc ttgctgccag catagcaggt attgcgcaag caaatccaga tagcatcggt
      181 acaaatgatt taccgttcag tccaatttta gaaagtggtt tgtctataag catggccccg
      241 cgtgctaagt atccagtatc ttcaagcagt ccaagtatta gaaataaaat aagtatctgg
      301 ggtagaaata ctaaaactga cccagtaccg ctgattatac catctgagat taaatcacta
      361 taccaggtat gccctaataa tcccgcagca gaaattgaga gaaagccgaa aaattcatca
      421 acaagttcca taaatggatt tgctaaccag aaaattgatg tgaatgtaaa tgccattatt
      481 agaaaaaaga ataataaacc ccactgtctg cttaataaaa atcgatctat ttttaaagtg
      541 cgttcatccg gtctattact tccatatttt aaagtagggt ttagaatttt taatttttca
      601 ttagcctttt taagattagg caaagtgatt tccttaacag gcactattac atttttttca
      661 atcctttcaa tatcctcata aaaacttaac agtctctctt tactgatatt attaaagact
      721 tgctgtaaag atcttgtgct ttctaaatct tgtgtgctca attttggctt tacacagctt
      781 aatagtttat ctatgccttt accagttcta ccatcaactt ttataacgcc gcatcctaat
      841 tcttcggcta atttattttc atcaatttcg taacctcttt tagctaggat gtcattcatt
      901 gtcagtacaa caattacatt aaaacctgat tcaattaatt gttttgcaag taataagtga
      961 cgtgaaatct ggctggcatc agctgtggct attaataaat gcggcgatcc aaatttcgga
     1021 tgattgtata gagaatttat cgagacttct tcatcgggtg agttagggtt taaactaatt
     1081 attccaggcg agtctaatac atctgcatct aatccaaatt tgctctgaat tttacaagtt
     1141 gaatattcta cagtagagcc gggataattt acagttttaa aattctttcc gcttagctga
     1201 ttaaataatg ttgtcttacc ggagttcggc ggacccacca atgtaataag atgtaacttc
     1261 tctgcggaag cggttttcat gacaatatta tacagtcagc ttcgtgttta cgaattgcaa
     1321 ttaaagtacc tcttaacgca aatgcaagag gatcgttaaa ggcagaagaa tttataagtt
     1381 ctatttcctg gccgggagta aaccctattt ctaataatct tctgaatgag gggtgtgaat
     1441 tatctatatc gctgataatt gctctttcgc caaaatttaa ttctgaagct gttttcataa
     1501 ttttaacata ttatttagat ttagtctata taaagttaag ctaactattc ataactgtca
     1561 agatatttta taagatttct tttaatctca tcaaaaacct accgggagat ttaacattat
     1621 tgtgtttaga gggtgaaacc gaaagcgaga acaagaagaa atagggtgta aatatagata
     1681 attcggttga tcataatcga taaaatggca gttttcttgg tttcatctgc attctttaat
     1741 tcgattttca attctcaatt cctaattatt atttcgtccc ggtaaaactt tcaagacttg
     1801 taaaattatt tacacttgta tgatttttac gtaattattg aattgctcaa cccataagta
     1861 gatatgtaag gaaatatttg gaaagtttgt tcagattata tctctttcta aaaatcattc
     1921 gatttaattc cataaagata tttttaaacg acatttttgt ctggtcccat tttccggttt
     1981 cttacttttt tacttgaatt atggttaatt acaatagcga caatcttgtc aaatcatggc
     2041 actaatcttg aaaaacacta atgcaaataa ttcattaaat aatttgaaat aatcggagat
     2101 agaaaatgga aatcgcaaaa ttagcatttc ttgaaaccta tacactcagc gaaaatggtg
     2161 gtgtaatggg agcaattttg gtaactgatg cagaaacaaa accattagaa tttagagtta
     2221 cagcacctat tcgtccaaca agttttcaaa aaactttgta cggcgatgtt ttacttgaac
     2281 atattctagt tgagttaatt tctgttccgc tgctaaatgc cgttaatgaa cagattgatc
     2341 ttatagttgt taaggatccg ctatttcttg gcgccaataa taaacaagga attagagtag
     2401 ttcggcttct taatgatgaa aaagaaaaat caagaagtaa tacatccgta gaattattga
     2461 atacaccact taatggctct gaaaaaggat ttgttgaaac atctaaaaag tttgaagagg
     2521 aactaaaggg aattaaagag cgtcttgaga agatagctga atcaagaaat ctttctgagc
     2581 cttttgaaag acttaaagcc gcttgtgaac aggttcagtt gcaacggaca aatgattaat
     2641 tatagtatta tcagatgatt cgcacagcaa gagaaaaaat tgctactaaa gtatccaaac
     2701 ttgaagtagc tgacgatttc catttcgcta aatggcttcg tcctcctaaa attcacaccg
     2761 ctgatgtaac agaactttat tctattgatg aggttgaaaa ttttcccgta gaacaattaa
     2821 cagcacccaa ttcaatcaaa ctaaaattcc gtttggcagc ttataagatt ccagaggtaa
     2881 ggcatagcta tttgcttgat aagccaaagg cgtatttgat tttctttggg ctgacacgtc
     2941 ctcgtctatt tacgatgaag gaaaaaatac tttctgcacc cgaaattttt aatattaaat
     3001 ccgcaattcc acaggcaccc gaaataattg atcaatcaga attatttatt gctaaggctc
     3061 ctgaattaat tgatcagtct tcaaaattta ttgcaactcc accggcttcg tatgataaat
     3121 cttctcagtt tttctttatc caggatccat catatgattt gaacttcaca ttttcaaatg
     3181 ttaaggtgca tgaaacacca ttgctcatgt caccgccaaa gattacagat gaactggcaa
     3241 accttactcc ggaaatttat aatatcgaat tgctgaatgt tgaaaagttt gatcacacat
     3301 taattaattc taatgatatt aatatagaaa agttcccaat ccctgcatta atcaaaattg
     3361 atgtacccgg tattgataat cttgttgaaa gtataacaga atcttatgct gtcccttttg
     3421 aagaaatgct gcaggctgat gtaaaatttt actcgccttc ttttgaaatt gcacatcctg
     3481 ttttcaaagt taaaattgag ctgccaagag cgatagttaa aaaagttgta attaaacaaa
     3541 aaaatcttga accgatcatt attgatgctc ttaatgatgc ttctgtttct gaatttgcga
     3601 agaacaatat tatgtcatta ttatcctctt tccgcgaact tagctggcaa gagtattcta
     3661 aaatgattaa ccctctgaca gaatatcaac ttcaatcatc tgaatttctt acttcgaata
     3721 attttgccgt tcttaatgat gagcttggct atgaaaaatt tgatcagaca tctgcagcac
     3781 ttggatacct ggctaaaaag ggtaatataa aatccgtact tttgattagc gacaatatta
     3841 ggtacaatga ttattggact ccttcattaa aggcattcgt aaaagacctt aaacttaaaa
     3901 aagttgaacc cggaatcacg aaaaaagtta aaggcacatc cgtaatctgg tttcttgatg
     3961 taaatgattt tggtaaaatt gaaattaaag actttgataa atttgatctt gttctgtttg
     4021 atgaactaat aaatataaaa tcagcagcag atcaggttac ccaacttatt accaaaattg
     4081 aacccggtta tatctggttt ttaactgcag tgataaatga taaagcagtt aaaaaatttc
     4141 ttgaggaatt tgactttacc tcaaaagtta cttttgctgc ctttggtaaa tcactagctg
     4201 atttacaggg agaagaaccc gtaacgacta ttaagaacat ttggttagaa ctggatgaga
     4261 tgcagtcttt tgaatattct gaagctctca atcaatccaa agaagaactt aatgttcttt
     4321 tggaaagccc aaatccatta aggttccaat ctaatatttt taatattatt catagactaa
     4381 aacagatact gaacttttca tcattccgta atattagtcc caaagcaaat ctgctaattg
     4441 aacaggtaga ggcgatagca aataataaaa agaaagccat gatatttaca cagtatgatg
     4501 taaatggaat gaagaaaatt gaaaaagcgc ttgaaatgaa taatgttaaa ttcgttgttg
     4561 gcagaaatgg aatgtcagct gaggaactta aacagactct tgatggattt tatgagcgac
     4621 gtgatgttac tgtattttta actaacctaa agccatctag aataaatatt aatcttacta
     4681 aagtacctta tatatttaac ttcgatcaat ggtggaatcc aattacgcaa tggcagaatt
     4741 atgatgaaat gggtatttcg gaaattgtgc attctccagt agtagtttat aactataaca
     4801 ttaaaaatac ttttgaagaa gaattgagca gtttgataaa ggaaaaaggt ttaagtaatc
     4861 gttacctgtt tgataatttg aagagtgaaa ctttatctga acttattaca atggaagatt
     4921 ggttgtacat ttttggtatg aacgcgcagt ttaataaaat gcttaatact gaaagaacca
     4981 aactagttaa aaagcttcag tcgattaatc taaataacta caaagcatta attaaatatt
     5041 tcttcagctt cctcggatat cgtgatataa gtataatgga tattgatgat gagcccatgt
     5101 tctatattat agggacagct agaaaaggta caacacctgt taaccttcac ggaaaatgct
     5161 tacttacaac agatgttaaa aaagaagact atgaagaagt tatacatttt aaaccatctg
     5221 caaatgagat taaaagaaaa ttcgtaatta ccaatggcga atttgctgag agaatcaaaa
     5281 acggcacgac ctacattgat tgcaaagatc tggcaaactt catattaacc ctaggtttaa
     5341 aatctcatgt taattctaaa aagaattaat atttagaacg atttgatact aaccttttat
     5401 tcttaactta aaatactcca tagtgttttt caaaccctca gacctttgaa ctttaggttc
     5461 ccagcccagg acacgttttg ctttggatat atcaggttgt ctaacctgag gatcatcgac
     5521 cggcaatggt tcataaatta ttttactctt tgaaccagtt aattcgataa tctcttctgc
     5581 catctgcttt acagttatct cttccggatt accaatgttt actggttctt tttcattgga
     5641 taacaataac cttaatattc cttcaacttc atcatctata tatataaagc tacgtgtctg
     5701 actaccatca ccaaaaactg tgattggctg gttgtttaat gcctgcgaaa agaaagcagg
     5761 tattgcacga ccgtcgttca atctcatccg agaaccaaat gtattaaata ttcttactat
     5821 tcttgtttct aatttatgat accgctgata agccattgta agcgcctctg caaatcgctt
     5881 agcttcatca tatactccgc gcggaccaat tggatttaca tttccccagt aatcctcttt
     5941 ctgcggatgc attaacggat caccataaac ctcagaagtg gaagcaagta gaaaacgtgc
     6001 acctttttct tttgctaatc ctaatgcttt atgagttcct aaggaaccaa ctttaagtgt
     6061 ctgtattgga agcaggtgat aatcaagtgg gctggcaggg gaggcaaagt gtaatatgta
     6121 atcaacactg cctgcaacgt gaataaagtt tgttacatcg tgatgtataa atttaaattt
     6181 aggatttcca aataagtgtt gtatgttgtc taaacttccg gttaataaat tatctataca
     6241 aactacattc attccctctt tcaataatcg atcacaaaga tgagaaccca aaaaaccaga
     6301 tccgccagtt ataactgctg tttgcatata aatcctttta agaattaaaa attaatagtt
     6361 tagaattaaa ttttatttta tagtaaattt agtgaaagat tatttaaagg gaattattaa
     6421 gaagaattct taataacttt aattaacaaa tcaaatttaa actcttctac agaatcctgg
     6481 agaaactttt tagtttcttt gtatacagaa aaaaaaactt atttatccct aacctctttc
     6541 aattcaaact taattgtgcc ataacctaaa atatcacggg caaaaccaag cattccgccg
     6601 ccttcaatcg atacaagcat tttaaccata ccataatctt ttgcatacca ttctgtcaaa
     6661 acatttttag ttccattaga agtttgcaga gttgtttcaa cttttagcgt ttcaaatgtt
     6721 ccagcgggca atgtgatggt ctcaaattta gtggctttgc ctttaatgtt tactgtatga
     6781 gtttcatcat taacaaattc ttttccatcc caggtccatt cttgaccgac ttgaacaggg
     6841 aagggaattc ttaaaagtgg tttatcataa acataattac cttcttttgt tataaataaa
     6901 agaagcttga ttttttgata ggtctcattt acaaaaagac ctttgtcatt tacaaaaagt
     6961 ttttgtcggt atttaaattt atctgaatca taactaaaaa gcttaacatt gttttctttt
     7021 gttactttta attcgagttc tccaaaactt gaatcatata tcaatgtttt ttttatttca
     7081 gtcggaaaat attcttggcc gaagcttttt tcttcagtta ccttaataga ctttgtagat
     7141 tctgcggttt gcgaattgat actgggacta ttaataatta aagttaaacc aataatgatt
     7201 atcgaaaagt agttcttcat atatctcttt tagaattatt attgtaacaa taccaagtaa
     7261 aatgtgtttt tgcaatgtag taaatcatct atccaattaa cttaattata tttgctccta
     7321 ctataaaaaa tgatttaaat tagggaaatg aattatcttg ataaactcga aaaagtaata
     7381 acacaaaact gttctattat cgcaaaagaa ctatatctta attcggctgt gttaatacca
     7441 atagttattt taaatgatac tgaatatgta cttttccaaa aaagatcttc tactgtaagg
     7501 caacccagtg aagttagttt tcctggagga cattatgatt caaattgcga taaagatttt
     7561 ctttccactg caattagaga aacttgt
//
LOCUS       DASWBY010000869        13924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_371048, whole genome shotgun sequence.
ACCESSION   DASWBY010000869 DASWBY010000000
VERSION     DASWBY010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 13924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13924
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371048"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /gene="dinB"
                     /locus_tag="VF870_15705"
     CDS             complement(<1..858)
                     /gene="dinB"
                     /locus_tag="VF870_15705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9253691.1"
                     /translation="MRTIFHLDLDAFFVSVERILDPKLNGKPVIVGGDPKFGRGVVAA
                     CSYEARAFGLHSAMPIRTAYKLCPQGIYIHGHGDEYTRFSKAVKIVLEQYAPLMEQAS
                     IDEFYLDMTGTQKMYGSMFGFATRLQKEIWDNISLPISIGIGSNKTIAKIGSDCMKPK
                     GITYIVPGMEKEFLSPMPVETIPGVGKVMKQSLNSRGIYRIGDISKLSSDYFGTAFGK
                     YGIDLWRKAQGEGTEYLTIQRMRKSISRETTFGNDVTSEEEIKNTLFSLTGKVAQSLR
                     KRGWEAATVD"
     gene            complement(889..1455)
                     /locus_tag="VF870_15710"
     CDS             complement(889..1455)
                     /locus_tag="VF870_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253692.1"
                     /translation="MKMTLFLISFLASFLFAQQNSNGSISMVFNNEKIDLPITTISIH
                     KDNGLILSFKAEIEDSIIYQMVKFELGFNELSSKLDAETLDGTRIDISTRNNKTNSGK
                     YLSMWFDGPSGEDNDYFEAANYGIFNKGEKISWGINSVSMQINITDVQYINGALHING
                     ELKGTFKSTSAPEGQVAEIKDCKFEVII"
     gene            complement(1683..2609)
                     /locus_tag="VF870_15715"
     CDS             complement(1683..2609)
                     /locus_tag="VF870_15715"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9253693.1"
                     /translation="MKPKYNIGTAGWSYKDWVPNFYPKNQSGGFDWLQFYSHYFNCVE
                     VNSTYYTYISPKVVDAWIKKVENSNDFIFHIKLHQDFTHKRKFDEQNIKSVRYNLDLL
                     NKSERLGGLLIQFPYSFSYDGNSVRHIQKLRDIFSDINCFVEVRHSSWKNNRALEFFK
                     ENDLTFCTIDQPQIGQAIEFNPIITNDKAYIRFHGRNVGAWKKSLSNFGKPQTYEEQS
                     SRYSYLYSPGELIEIEQKIKLIQQKVKEVNVIMNNHPQGDAVANAFELIHLLDEKTKV
                     EMPDSIVKAYTRLEEIFLNDSTLISNYSYCVK"
     gene            complement(2658..3281)
                     /gene="lexA"
                     /locus_tag="VF870_15720"
     CDS             complement(2658..3281)
                     /gene="lexA"
                     /locus_tag="VF870_15720"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX9253694.1"
                     /translation="MQKELTAIQKNILDYLIDQIKGKGIPPTLADVAKHFGYKNRATV
                     QQHFQAIEKKGYIKKNPKLSRGIELTLEDKYFVPRPILGEVAAGNPLTIYPDAIDTVQ
                     LPTIARMPQDSFLLRVKGDSLKDAYIFSNDIVIVNPNLDPKNGQIVVAILDDAAVVKR
                     FYKKRSEIELVSENPEYKPIVINKKYSSFKVVGIVVGIYRSMDKKAG"
     gene            complement(3417..6992)
                     /locus_tag="VF870_15725"
     CDS             complement(3417..6992)
                     /locus_tag="VF870_15725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9253695.1"
                     /translation="MEIKNKEWLDILGRNSDYHGDALVILDDSFEIVFTNQKAATLFF
                     IDDFNISLEQVFEKETVQELTDIIGPVINSFQKKSYKNFSVNFKTGNSAVFDLFVEPI
                     QADSITNILIVFRNSENVNANEFLSRIKTDSLKNLLADKVNPVTILISELQKLAPFTI
                     ISLKRIQNLIDQNEYPIWVKDVESKLIAINNSYASILNVETSFAAGKKHETFLPPNQK
                     IIYKMMDQYVILNLQQIILDGLSKKVKNIGVYKSVIQIPIVDNFNKILAIVGLIVDNK
                     DDYYNFWGEKEFFGSLVNASSKPSAFLSSDGVIEGSNNRFKEFFNVENEQIVGKRIEE
                     ILSANVVNKINSFIQNDLTEESISLIEETDVSESSVNNDQLLLLKISNLKKYKSNIFI
                     SVETPDEIEKSENELQNILMHRGTMFETLIQKNPEPIFVYEKENLKFLEVNDAAIKLY
                     GYNRDEFLQMDLTDLYAPEDIQTLLDSFGDESSEARFSKPFRHRKKDGSNILVEISKT
                     SFRYNDKEAHFNIIKDITDSVEKDKQYQMLRAIFNESDLLVFNTDASGFIKFINNNVI
                     KNLGYKSEEILQLSFASLVSDDDRGIVNTSIFQSHIKDSVILESKIKDSLGNYFDAEI
                     YASPILDFDGGIDSFTIIVKRSSEKLVAEKPKEIIKEVVKEVFVERPGSTSSQKTIIP
                     DSNFLSGMFHEILTPINVIIGFSQELISSTENPTEEQLEASEIINQNRIKMMDTMNAV
                     VEYSDIMQNKSPLKIEDITITDVIEKLDDNIKDITGINDIQFAYGKISSSLRFKSDKQ
                     KFENFILSLIKVVSRLSKDKKVYFSAFSVDAETFLIGISDQYGNPSEYVSNVLEQVFV
                     NDKDPKDFGLPKLTTYLARILLKLLDGKFYKSSKDTYRKETGFIFPIVLIAGPENKVY
                     ESVQEVISSTYSSEDFNEQTDNIQAQNDIAREEESEENLDQLVDQNKDENYTKDDTAE
                     LLSDESDDIFKPVQPIAQELLSKIDDEKYIENDSLQQEIESFSISDQNQTEELIDNEY
                     NKKGSDIQENLSDEIKAELESDTDNFQQVKQPQSLDLTKLKCLYIEDQVDSQILFKVQ
                     MKELHDVKFAVSFEEAQVVLLNHHFDFIVMDINLQGEYNGLDALKIIKTMPAFSSIPI
                     IAVTAYVLPGDKEKFIAAGFDDFISKPIFKEKMIESLEKIFLSKD"
     gene            complement(7011..8279)
                     /locus_tag="VF870_15730"
     CDS             complement(7011..8279)
                     /locus_tag="VF870_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX9253696.1"
                     /translation="MNKNFTTRGKAFTVFIIGLLFISQSCADLSSDPNQNSNQSNSKI
                     QISSPANNGTFTEGNNEIIYSLAQPYSIKFLELYVNDTFVKNIPPNSNGTAPKVNIQL
                     DSTYIGQKLSLYLIYYDNDGTSQRSNKVTEVLVTKDNRIPFKPYNITLLKFNDGSVNI
                     SWKDSSKFVEKYELWRKINLDGEYLLHKELSGKSNNTNDENLDTSKIYFYKVRGYKSS
                     GFSEFSNEVNTKGIVTSGNLNPPSDLYASVSGISSVVLNWKDNSDNENYFAVQRSISG
                     GIFTRVAAVTKNTTTYLDSGNGLVVGATYLYRIVAYSNEDSAISNTVSIKITTGILLP
                     PTNLTATYNSTVRVIQLNWNSIDNTILYFDIERKTDSGAYTLLRRIPAVDKLYLDFTV
                     EQNKVYTYRVRGYDLNRFSEYSNEVTISTF"
     gene            complement(8315..9937)
                     /locus_tag="VF870_15735"
     CDS             complement(8315..9937)
                     /locus_tag="VF870_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253697.1"
                     /translation="MKKHFKIFPTLIILCGILFLQNSVLAQPKIAVLYSRLTEKIDNT
                     TSNQVFNAITNWELFLMQDKIPYSVIYDEDIESGIEDEFDILILPSVNFISNEQMEEL
                     QKYMTSGNSIICSGSKLLFQDKSLTDFQNLKTLFGLNDIKTIPTENMSYLHSLVPSPI
                     NQFNAYDDKILQIANKNQTLFCGKIDSKVSACGFILGENDFNSSESSILYGVVGSSRF
                     LWTGFDLGDIIGGNSDMQTFKRLISDAISWMDNPSDTYIANFNESLSAPVIVTLQYNN
                     ALESELVDILKKNNIQPILIISPDQKVSKEIFNKFANDEIVLDLSGNSKLTSKSTVEF
                     INNFNQDNEVNISTILVDKQFLEINDLILISENGISKMCYIDQAPGIPELLNKDLLTI
                     PFVTSKDVPKSREVINYLNYIPKVNCDINPEDELLKQINQIKSSKYNFVTPESLKKWW
                     SVKQKITTEIKYINDNEIEIWLSNNNLSKVSELEVFLNYINKIDKKSLTISLNNSLLE
                     YSFDDASGAVVINLENILPNSLNKIKIKFDLE"
     gene            complement(9957..11855)
                     /locus_tag="VF870_15740"
     CDS             complement(9957..11855)
                     /locus_tag="VF870_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9253698.1"
                     /translation="MAVKNFFGIRINQFFIFVSGFVILISAILVYYFILRGIYGRFDT
                     SEILPNTNIIEDFLFGKQPEVAILYSKYTENTLPVGSTWLNDNISTWKKFLDNLDLKY
                     EIISDQKIENGDHQKYKLLVLPGTKSLSEKEALQIKKYLENGGSVFATGGIGTYSNDG
                     KWRGWEFLNQVFGIQFSHELKPDEFSKIHTVCGSLPITANIPTGFPLKVATWDRPMAV
                     EVLDPRTTQVSFWYNYRMDDGLVREEIKKSAGIAYGNFGRGRFMWMGFELNSIIGVQE
                     DYIFFERLFNNSIKWLMYFPIAYVRDWPTGYEAAAVVAPVLTNDVNNINNILPIFKSE
                     GISATFFVDPVTAEINRELVRKISSYGEIAALVDIGYLASINDKFNKLNSYSEQLKKL
                     NAAKQKLEKISSSSVSGFLPYYGLFDQNTLNAVINADYKYVLTDSLTDRSVPKTIIHG
                     DKRIVTMTKTARDDYEIIRDFGLTLPEFQFYTYQEDLDRVLFEGGMYILKLHTEYQCR
                     SDYANVLKDVIKDLKKKKFWIATANEIQKWYEKRDYIEIKADRRGKTRVALKVSNPGI
                     ESINNLVIDLDLNDFAQNISIGSEIIGTKTASVKHTAGSQLVYLYIDDLKPGESRQYY
                     IDYDKASF"
     gene            complement(11846..12298)
                     /locus_tag="VF870_15745"
     CDS             complement(11846..12298)
                     /locus_tag="VF870_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253699.1"
                     /translation="MELIPILSTIILVATICTFILAIGAYILYKVREGKPQYGYQTKP
                     ASVQGELVVPSEAAYTKDSTRLDRGIIKYVEPGITKEEYTKQRQPSQSKYTPEPRGYR
                     IEEEKFEKQKVRVRPQERFSDENKFLKYTSEGYIPTQEDRSSGEVKWR"
     gene            complement(12307..13731)
                     /locus_tag="VF870_15750"
     CDS             complement(12307..13731)
                     /locus_tag="VF870_15750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9253700.1"
                     /translation="MRINQLVDRVKINISSREDEKKGENLEQKYLRRILVSGIVSLLL
                     ILVIVYTLPYTILSYSSLFEYSTIIALVIFLFILLVRYFAILIMAYLYINEYTFSNTN
                     GYAPFVSIIVPVFNEGKIIRKSILSLLNLDYQNYEIIIVNDGSTDDTKQAAESMVGFQ
                     EGKYGKIKISLINQPNLGKARALNAGIYFSKADFVLCMDGDSQLAPESIKVAVRHFKN
                     PKIGAVAGNVKVLNRKRFFTDLQALEYIEGLNMARSAQSYVKLVNIIPGPIGIFRRKA
                     IEQAGFYSSDTYAEDADLTLKILSKGWKIYYEPKSISYTEAPVTLQQLLKQRYRWTRG
                     ILQSIRKHKKIMLNPTVNFGQTFILWIMSYEALIWPTMNIAANAFFIFAALFFGFSHL
                     IFFWWAGLAILDLVTALYCVAVEKEEFRLIWYAIIYRMFFILIIDICKTMSTIEEFLG
                     IRMTWGKLERSSSPKLNTIAVNNN"
BASE COUNT         4522 a         2411 c         1954 g         5037 t
ORIGIN      
        1 atcaacagta gccgcctccc atcctctttt ccttaaagat tgagcaactt ttccagttaa
       61 actgaacaat gtatttttaa tctcttcctc acttgtaaca tcatttccaa aagttgtttc
      121 gcgcgaaata ctttttctca ttcgctggat tgttaaatat tctgttcctt caccttgagc
      181 ttttcgccag aggtctattc cgtatttccc aaaagctgtt ccaaaataat ctgatgaaag
      241 ttttgagata tcaccaattc tataaattcc ccgtgagttt aaactttgct tcatcacttt
      301 accaacaccg ggaatcgttt ccaccggcat tggagataaa aattcctttt ccattccggg
      361 aacaatgtag gtaatccctt tcggcttcat acaatctgag cctatttttg ctatggtttt
      421 gttgcttcca attcctatag aaataggtaa acttatatta tcccaaattt ctttttgcag
      481 tcttgtagca aagccaaaca ttgaaccata cattttttgt gtaccggtca tatcaagata
      541 aaattcatca attgaagctt gttccatcaa aggagcatat tgctctaaaa caatttttac
      601 tgcctttgaa aatcttgtgt actcatctcc atgtccgtga atataaattc cttgtggaca
      661 aagcttgtag gctgttctga taggcatagc agaatgtaaa ccaaaagcac gtgcttcata
      721 agagcaggca gctactactc ctctgccaaa ttttggatca cctccaacaa taaccggttt
      781 gccattaagc ttaggatcta gaattctctc tacagaaaca aagaaagcat caagatctaa
      841 atgaaaaatt gtacgcatag tttaagtcaa aattaaaaat caaagaagtt aaataattac
      901 ttcaaatttg cagtctttta tttctgcaac ctgcccctct ggagctgagg ttgatttaaa
      961 ggttcctttt agctcgccat ttatgtgcag tgctccattt atatattgaa catctgtaat
     1021 attgatttgc attgacacag aattgattcc ccagctaatc ttttcccctt tattgaaaat
     1081 accgtaatta gctgcttcaa aataatcatt atcttctcca gagggaccgt caaaccacat
     1141 agataaatat tttccagaat tagttttatt atttcttgtg ctaatatcaa tcctggtacc
     1201 atcaagagtt tcggcatcca gctttgatga tagttcatta aacccaagtt caaactttac
     1261 catctgataa ataattgaat cttctatttc tgctttaaag cttaatatta aaccattatc
     1321 cttgtggatg gagatggtag ttatcggtag atctattttt tcattattaa aaaccatact
     1381 tatactacca ttagaatttt gttgtgcaaa caggaaagaa gctaaaaatg agattaaaaa
     1441 taatgtcatt ttcattttaa cacctcttaa tttttaatta aacatgatga ctaaatattt
     1501 attttgatat ttttgagaaa catgaaaatc ttatgaatgt ctttaattca caatgctcat
     1561 atacttattg aatttcttaa tccttaaaaa ttcgatgatt aagttcactt gtcatgaagt
     1621 aatcataaaa ttataagaaa ctattgataa taagttgttg tataagcgat aaaaaatctt
     1681 aattatttta cacaataact gtaatttgag atcaaggttg aatcattaag aaaaatctct
     1741 tccagcctgg tataagcctt tacaattgaa tccggcattt ccacttttgt tttttcatca
     1801 agtaaatgaa tcagttcaaa agcatttgcc actgcatctc cctgtggatg attattcata
     1861 atcacattta cttctttaac tttttgctgt attaatttaa tcttttgttc aatctcaatt
     1921 aattcacctg gagaataaag ataactgtaa cgcgaacttt gttcttcata agtctgaggc
     1981 ttaccaaagt tagataatga ttttttccaa gctccaacat ttcttccgtg aaatcggata
     2041 taagctttat cgttagttat tattggattg aactcaattg cctgcccaat ttgaggctga
     2101 tcaattgtac aaaaagtcaa atcattttct ttaaagaatt ctaatgcccg attgtttttc
     2161 caggaagagt gacggacttc cacaaagcaa tttatatcgg aaaaaatatc tcttaatttt
     2221 tgaatatgcc gaacagaatt tccatcataa gaaaaggaat aaggaaactg aatcaataac
     2281 ccgccaagtc gttctgactt atttaacaga tctaaattgt aacgaacaga ctttatgttt
     2341 tgttcatcaa attttctttt atgtgtaaaa tcctggtgaa gtttaatatg aaaaataaaa
     2401 tcatttgagt tttcaacttt ttttatccag gcatcaacaa ctttgggact tatataagta
     2461 tagtaagttg agttaacttc aacacaatta aagtaatgcg aataaaactg aagccagtca
     2521 aatccaccag attgattttt gggatagaaa ttcggcaccc agtctttata agaccagcca
     2581 gcagtaccga tattgtattt tggtttcatt ttaattctat ttctcccccc ataagaatgg
     2641 actggaaata aattctccta accagctttc ttatccatac ttctatatat cccgaccact
     2701 ataccaacaa ctttaaaaga tgaatatttt ttattaatta caatcggttt gtattccgga
     2761 ttttctgaaa cgagttcaat ctcacttcgc tttttataaa atctttttac aactgctgca
     2821 tcatcaagaa ttgcaactac aatttgccca ttctttggat caagatttgg atttacaatt
     2881 actatgtcat tgctaaatat gtaagcgtct tttaagctat cgccttttac acgaagcaga
     2941 aaagaatctt gaggcattct ggctattgta ggaagctgca ccgtatcaat tgcatcagga
     3001 taaattgtta atggattgcc agcagctact tctccaagaa ttggtctcgg aacaaaatac
     3061 ttatcctcta aagtaagttc tataccccta gaaagttttg ggtttttttt aatgtatcct
     3121 tttttttcaa tcgcttgaaa atgctgttga actgttgcgc gatttttata tccgaaatgt
     3181 tttgctacat cagctagtgt tggcggaata cctttccctt ttatctgatc tatcaaataa
     3241 tctaaaatat tcttttgaat tgcagttagc tctttttgca taattacacc tatatataat
     3301 aattgccata catttgtatg ctaacttaat tcgaaaatgg aaaaatgtca agagaggaat
     3361 aaaaaaaccc cttaattctt agaattaaag ggtttaaaca taaaatgcaa aataatttaa
     3421 tcttttgata ggaatatctt ttctaacgat tctatcatct tttctttaaa gattggctta
     3481 gagataaaat catcaaatcc agcggcaata aacttttctt tatcaccagg caaaacgtac
     3541 gcagtaactg caataattgg aatagaacta aaagccggca ttgttttaat tattttcaat
     3601 gcatcaagtc cgttatactc gccctgcaaa tttatatcca taacaataaa atcaaaatga
     3661 tgatttagaa gtacaacctg cgcttcttca aaacttactg caaacttaac gtcatgtaac
     3721 tctttcatct gaactttaaa gagaatctgt gaatcaacct gatcttcaat ataaagacat
     3781 tttagttttg ttaaatctaa agattggggc tgtttaacct gctgaaaatt atcagtatca
     3841 gattccaatt cagctttaat ttcgtcagaa aggttttcct gaatatcaga tcctttttta
     3901 ttgtattcgt tatctattaa ttcttctgtt tgattttgat cagatatact aaaagattcg
     3961 atttcctgct gtaatgaatc attttcaata tacttttcat catcgatttt tgatagcagt
     4021 tcctgtgcaa ttggttgaac aggtttaaag atatcatcag attcatcaga taataattct
     4081 gcagtatcat cttttgtata attttcatct ttattttgat caactaactg atccaaattc
     4141 tcttctgatt cttcttcccg ggcaatatca ttttgtgcct gaatgttatc agtctgttcg
     4201 ttaaaatcct ctgaggagta tgttgaggat atgacttctt gaactgactc ataaacttta
     4261 ttttctgggc ctgcaataag tacaattgga aaaataaatc ctgtttcttt tctataagta
     4321 tctttagaag atttatagaa ttttccatct aataatttta aaagtattct tgctaagtat
     4381 gtcgttaatt ttggtaaacc aaaatcctta ggatctttat catttacaaa gacctgctcc
     4441 agtacatttg aaacatattc tgatggattg ccatactgat cgcttattcc aataagaaat
     4501 gtttcagcat caacagaaaa agctgagaag taaacttttt tatctttact taatcttgat
     4561 acaactttta ttaaggaaag aataaagttc tcaaatttct gtttatcgga tttaaacctt
     4621 agtgatgatg atattttacc gtacgcaaac tggatatcat ttattcctgt tatgtcttta
     4681 atgttatcat ccagcttttc aataacatct gttatcgtta tatcttcaat ttttaatggc
     4741 gatttattct gcattatatc agaatactca acaacagcgt tcatcgtatc catcatttta
     4801 atgcggttct gatttatgat ctcagaagct tccaattgct cttcagttgg attttctgta
     4861 ctgcttatta attcctgaga aaaaccaatt ataacattaa tcggggttaa aatttcatga
     4921 aacatacctg aaagaaagtt tgaatcaggt attattgttt tctgtgatga tgtactacct
     4981 ggtctttcaa caaaaacttc ttttacaact tcttttataa tttcttttgg tttttcagca
     5041 actaattttt ctgatgaacg ctttacaata attgtaaagg aatcaattcc gccatcaaaa
     5101 tcaagaatcg gagatgcata aatttcagca tcaaaataat ttccaagcga gtctttaatt
     5161 tttgattcga ggataacaga atctttgata tgcgattgaa atattgaggt gtttactatt
     5221 ccacgatcat catctgaaac aagacttgca aaggataatt gaagaatttc ttcgctctta
     5281 tagccaagat tttttattac attattatta ataaatttta taaaaccaga agcatctgta
     5341 ttaaatacca gtagatcaga ttcattaaat atagccctaa gcatctgata ttgtttatct
     5401 ttttcaacac tgtctgtaat atccttaata atattaaagt gtgcttcctt atcattatac
     5461 ctaaatgaag ttttgctgat ttctacaaga atgtttgatc catctttttt tctgtgcctg
     5521 aatggtttac tgaatcttgc ctctgaggat tcatcgccaa aagaatctaa taaagtttga
     5581 atatcttccg gagcataaag atcggtaaga tccatttgaa gaaattcatc acggttgtaa
     5641 ccgtataatt ttattgcagc atcgtttact tcaagaaatt ttaaattttc tttttcataa
     5701 acaaaaattg gttcaggatt tttttgaatt aatgtttcaa acattgttcc tctatgcatt
     5761 aaaatatttt gtagttcatt ttcagatttt tctatttcat ccggtgtttc aactgaaata
     5821 aaaatattag atttatattt ttttaaattc gaaattttta aaagcagcag ttggtcgtta
     5881 tttacagaag actcagaaac atcagtttct tcaatcagac taatagattc ttctgtcaaa
     5941 tcattttgaa taaaggaatt gattttatta acaacattag cagataaaat ctcttcaatc
     6001 cgcttaccaa ctatctgttc attttcaaca ttaaagaatt ctttaaatct attattactc
     6061 ccttcaatca ctccatctga actcaaaaat gctgagggtt ttgaggaagc atttactaat
     6121 gaaccaaaga attctttttc accccaaaag ttataataat catccttatt atcaacaatt
     6181 agaccaacta tagcaagtat tttgttaaaa ttatctacaa tcggaatctg aataacgctt
     6241 ttataaactc caatattttt aacttttttg ctcaatccat ccagaattat ttgctgcaga
     6301 ttaagaataa cgtattggtc catcatttta taaattatct tctgattcgg cggtaaaaat
     6361 gtctcatgtt ttttacccgc ggcaaaagaa gtttcaacat ttaaaatgct tgcataagaa
     6421 ttatttatag caataagttt actttcaaca tccttaaccc aaatcggata ttcgttctga
     6481 tcgattaaat tttgaattcg ctttaaacta attattgtaa atggagccag tttttgtaat
     6541 tcagaaatta agattgttac aggattaact ttatcagcaa gaagattttt taacgaatca
     6601 gttttaattc tgcttaaaaa ttcattagcg ttaacgtttt cactgtttct gaaaacaatt
     6661 aaaatattcg tgatgctatc tgcttgaatt ggctctacaa ataaatcaaa tactgcacta
     6721 ttaccagttt taaaattaac agagaagttt ttatatgatt ttttttgaaa tgaatttatg
     6781 actggcccga ttatatcagt tagttcctga acagtttctt tttcaaaaac ttgttctaaa
     6841 gaaatattaa agtcatcgat aaaaaataac gtggcagctt tttgattagt aaaaacaatc
     6901 tcgaatgaat catccaaaat aacaagtgca tcgccatggt aatctgaatt tctgccaaga
     6961 atatcgagcc actctttatt ttttatttcc accgtaataa aaccaattta ttaaaacgtt
     7021 gatattgtca cttcattaga atattctgaa aatctattca gatcatatcc tctgactctg
     7081 taagtgtaaa ctttattttg ttcaactgta aaatccagat acaatttatc gactgctgga
     7141 attcttctta aaagagtata agcacctgaa tcagtttttc tttcaatatc aaaatacaaa
     7201 atggtattgt ctatactatt ccaatttaac tggattactc tgactgtact gttgtaagta
     7261 gcagtaagat tagtcggagg taataatatt ccagtagtta ttttaatact aactgtgttg
     7321 cttattgctg aatcttcatt actgtatgca acgatcctgt aaagatatgt tgcgccaact
     7381 actaaaccgt ttccggaatc caaatatgta gttgtgttct tagttactgc tgcaacccgt
     7441 gtgaaaatac ctccagaaat acttctttga acagcaaaat agttttcatt atcactgtta
     7501 tctttccaat ttagaaccac agaggaaatt ccagaaacac ttgcataaag atcgctagga
     7561 ggattaagat ttccagatgt tactattcct tttgtattta cttcattact aaactcggaa
     7621 aatcctgaac tcttatatcc gcggacttta taaaaatata ttttgctggt atctaaattt
     7681 tcatcattag tgttattaga cttacccgaa agttctttat gcagcaagta ttcaccatcc
     7741 agatttattt tgcgccacaa ctcatacttt tcaacaaatt tagaagaatc tttccagctg
     7801 atattaacag agccatcatt aaattttagt aatgtgatgt tatatggttt aaatggaatt
     7861 ctattatctt ttgtaaccaa tacttctgtt accttattac ttctttgcga tgtgccatca
     7921 ttatcataat aaattaaata taggcttaac ttttgtccga tatatgttga atcaagttgg
     7981 atgttcactt ttggtgcagt gccgttacta ttaggcggaa tattttttac aaaggtgtca
     8041 ttcacatata attcaagaaa ttttattgag tatggttgtg ctaatgaata tataatttca
     8101 ttattacctt ctgtaaaagt tccattgttt gcaggtgaag aaatttgaat ttttgaattg
     8161 gattgatttg aattttgatt tggatcagaa cttagatcag cacaagattg cgaaataaat
     8221 aaaagaccaa ttataaatac cgtaaatgct ttccctcttg tagtaaaatt tttattcatt
     8281 ttttatctaa acgtaattgg tattattcca aagtctattc cagatcaaat ttaatcttaa
     8341 ttttatttaa tgaattaggt agaatatttt ctaaattgat cacaactgca ccggaagcat
     8401 catcaaatga atattcaagt aatgagttat ttaaagaaat agttaaagat tttttatcaa
     8461 ttttatttat atagtttaag aaaacttcaa gctcactaac tttagataga ttattattac
     8521 tcaaccaaat ctcaatctca ttatcgttaa tatatttaat ctcagttgta attttttgtt
     8581 taacactcca ccattttttt aatgattcag gagtaacgaa attatatttg gatgatttta
     8641 tttgattaat ctgttttagt aattcatctt ctggatttat atcacaatta accttcggta
     8701 tataattcaa ataattaatt acttccctgg attttgggac atcttttgaa gttacaaatg
     8761 gaattgttaa caaatcttta ttcaataatt caggtattcc aggtgcctga tcaatataac
     8821 acattttact gataccattc tcacttatca agatcaaatc attgatttct aaaaattgtt
     8881 tatcaacaag aatagtggat atatttactt cattatcttg attgaaatta ttaataaatt
     8941 caactgttga tttagaagta agcttactat ttcctgatag atcaagaact atttcatcat
     9001 tggcaaactt gttaaaaatc tcttttgaaa ctttttgatc agggctaata ataagaattg
     9061 gttgaatgtt gtttttcttt aaaatgtcaa caagttcaga ttcaagagcg ttattgtact
     9121 gaagtgtaac tattacagga gcggataaac tttcattaaa atttgcaatg taggtgtcag
     9181 atggattatc catccaacta attgcatctg agattagtct tttaaaggtc tgcatatctg
     9241 agttaccacc aattatatca ccaagatcaa aaccggtcca taaaaatcta ctgctgccaa
     9301 ctactccata cagaatagaa gactcacttg aattaaaatc attttcacct agaataaatc
     9361 cgcaagctga aaccttacta tcaattttac cacagaacaa ggtctgattc ttatttgcga
     9421 tttgtaaaat tttatcatca tatgcgttaa actgattaat ggggcttggc acaagtgaat
     9481 gaagataact catattttct gttggaatag ttttaatatc atttaatcca aatagtgttt
     9541 tgagattttg aaaatcagtt aaacttttat cttgaaacaa taattttgat ccagaacaaa
     9601 ttattgaatt accacttgtc atatactttt gcaattcctc catctgctca tttgaaataa
     9661 aattaacaga aggtaaaatc aatatgtcaa actcatcttc aattccggat tcaatatctt
     9721 catcataaat aactgaatat ggaattttat cctgcattaa aaataactcc caattggtta
     9781 ttgcattgaa aacctggtta gaagttgtgt tgtcaatttt ttcagtcagc ctggaatata
     9841 aaactgcaat tttaggttga gctaaaacag aattctgcag aaataaaatc ccgcatagga
     9901 taattagggt tggaaatatt ttaaaatgct ttttcaattt gtaatttcat ttgctattaa
     9961 aagctagcct tgtcataatc gatataatat tgtcttgatt caccgggttt taaatcatct
    10021 atatacaaat aaaccaattg tgagcctgct gtatgtttta cgcttgctgt tttagtcccg
    10081 attatttctg aaccaattga gatattttgg gcaaaatcat tcagatcaag atcaataacc
    10141 aaattgttta ttgattcaat tcctggattc gagactttta atgcaacgcg ggttttacct
    10201 cttctatcag ctttgatttc aatataatct cttttttcgt accatttttg tatttcattt
    10261 gccgttgcaa tccagaattt tttcttttta agatctttga ttacatcttt taaaacattt
    10321 gcataatcac ttctgcactg atattctgta tgaagtttta agatatacat tccgccttca
    10381 aaaaggacac gatctaaatc ttcctgataa gtatagaact gaaattccgg aagtgtcaat
    10441 ccaaaatctc tgataatttc ataatcatcc cgagcagttt ttgtcatagt aacaattcgt
    10501 ttatccccat gaattatagt ttttgggact gatcgatctg taagtgaatc tgtaagaaca
    10561 tatttataat cagcattaat aaccgcattc aatgtattct gatcaaacaa gccgtagtat
    10621 ggtaaaaaac cagagacgct gcttgaagaa attttttcaa gtttctgttt tgcggcatta
    10681 agttttttta actgctccga gtaagaatta agtttattaa acttatcgtt tatcgaagct
    10741 aaataaccaa tatcaactaa tgcagctatt tcaccgtacg aagaaatttt tctaacaagt
    10801 tctctgttga tttcggctgt cacaggatca acaaaaaagg ttgcagagat tccttcactt
    10861 ttaaatattg gtaaaatatt atttatgtta ttgacatcat ttgtaagtac aggcgcaaca
    10921 acagctgcag cttcatatcc ggtgggccaa tctcttacat aagcgatagg aaaatacatc
    10981 agccatttaa ttgagttatt aaaaagtctt tcaaaaaaaa tgtaatcttc ctgcacaccg
    11041 atgatagaat ttaattcaaa tcccatccac ataaatctgc ctctcccaaa attgccgtaa
    11101 gctattccag cagatttttt tatctcttca cgaacgagtc catcatccat acggtaattg
    11161 taccaaaaac taacctgcgt tgttcttgga tccaaaactt caacagccat aggtctatcc
    11221 caggttgcaa cttttagtgg aaagccggtg ggaatgttag cggttatcgg taaacttccg
    11281 cacacagtat gaatttttga aaattcatcg ggtttaagtt catgggaaaa ctgtatacca
    11341 aaaacctgat ttagaaattc ccaaccgcgc cattttccat cattagaata cgtacctatt
    11401 ccacctgtag caaatacact tccgccgttt tccagatatt ttttaatctg cagtgcttct
    11461 ttttcactta atgattttgt ccccggcaaa actaataact tatacttttg atgatcccca
    11521 ttttcaattt tctgatctga aattatttca tatttcagat caagattatc aagaaatttt
    11581 ttccaggttg atatgttgtc atttaaccag gtactgccaa ccggcaaagt attttctgta
    11641 tatttggaat atagtatagc tacttcaggc tgtttaccaa ataaaaaatc ttcaataata
    11701 tttgtatttg gaagaatttc agatgtgtcg aatcggccat agataccgcg aagaataaaa
    11761 taataaacga gaatcgctga gataagaata acgaaaccag agacaaagat aaaaaattga
    11821 tttatcctta taccaaagaa attctttacc gccacttcac ttcccctgaa cttctatctt
    11881 cctgagtggg tatataaccc tcagaagtgt atttcaggaa tttattttca tcactaaatc
    11941 tttcttgcgg tctgacccga accttttgtt tttcaaattt ttcttcctca attctatatc
    12001 ctctgggttc tggtgtatat ttagattgtg atggttggcg ctgttttgtg tattcttctt
    12061 tcgtaattcc cggctcaaca tatttaatta tgccacgatc taaccgggtt gaatcttttg
    12121 tataagcagc ttctgatgga acaacaagtt ctccttgcac agatgccggc ttagtttgat
    12181 atccgtactg aggtttcccc tcgcgaacct tgtaaaggat atacgccccg atagccaata
    12241 tgaaagtaca tattgttgct acaagaataa tggttgaaag tatcgggata agttccattt
    12301 aatttcctaa ttattattaa cagcaatagt atttaattta ggtgaactac ttctctctaa
    12361 ttttccccat gtcattctta ttcctaaaaa ctcttcaatt gttgacattg ttttacaaat
    12421 atctataatc aaaataaaaa acattcggta aatgattgca taccaaatta atctaaattc
    12481 ttctttttct actgctacac aataaagcgc agttacaaga tccagaattg ctaatccggc
    12541 ccaccagaaa aatattaagt gtgaaaaacc aaaaaataat gctgcaaaaa taaaaaatgc
    12601 atttgctgca atattcatag tcggccagat caatgcttca taagacataa tccataaaat
    12661 aaatgtttgt ccaaaattta cagtaggatt taacattatt ttcttatgtt ttcgtatcga
    12721 ttgaagaatt cctcttgtcc atctatatct ttgttttaaa agctgctgca gggtcactgg
    12781 tgcttctgtg taagaaatag attttggttc ataataaatc ttccaccctt ttgataaaat
    12841 ctttaaagtt aaatcagcat cttctgcata agtatcactt gagtaaaatc cagcctgctc
    12901 aatagctttt cttctaaaaa tgccgatggg ccccggaata atgttaacaa gtttaacata
    12961 actctgtgcg cttcgagcca tatttagtcc ttcaatatac tccagcgctt gaagatcagt
    13021 aaaaaatctt tttctgttca gtacttttac attacctgca accgccccta ttttaggatt
    13081 tttaaaatgt cttactgcaa ctttaattga ttcaggcgct aattgcgaat ctccatccat
    13141 gcaaagaaca aaatcagctt ttgaaaaata aatacctgca tttagtgcac gagctttacc
    13201 caaatttggc tgattgatta atgaaatttt tatctttccg tatttgcctt cctgaaatcc
    13261 aaccatagat tcagcagctt gcttagtatc atctgttgaa ccatcattaa ctattatgat
    13321 ttcataattt tgataatcaa gattcagcaa agatagaatt gatttcctaa ttattttgcc
    13381 ttcgttaaac accggaacaa ttatggaaac aaaaggagca taaccatttg tattggagaa
    13441 agtgtattca ttaatatata aatacgccat aatcaaaatg gcaaaatatc ttaccagaag
    13501 gatgaaaaga aaaattacaa gagcaatgat tgttgaatac tcaaaaagtg aactgtatga
    13561 caaaattgtg taaggtaaag tataaactat tactaggatt agtaaaagag aaacgatacc
    13621 gcttactagt attcttcgta aatatttttg ctctaggttt tctccttttt tttcatcttc
    13681 ccggcttgaa atattaattt ttacacgatc tacaagctga tttattctca tttttccggt
    13741 tgatggtttg tgtcaatttt tcaataataa tgccatcatc ttcagtttat ttaactttta
    13801 attctaataa attaacaaaa tatcattacc aatattggac tttaatttaa tctgtatcat
    13861 tatcaaacaa gggtgataat taagttttgg agataaaatc ttaaaaatag ctggttacaa
    13921 tccc
//
LOCUS       DASWBY010000870         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_74375, whole genome shotgun sequence.
ACCESSION   DASWBY010000870 DASWBY010000000
VERSION     DASWBY010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74375"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..632
                     /locus_tag="VF870_15755"
     CDS             111..632
                     /locus_tag="VF870_15755"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9253701.1"
                     /translation="MHFKDLLLLLGLSIILVYMVMAAQFESLLDPFIIMFSVPFAFSG
                     VFIGLFVTGTAFSVIAFLGSVILVGIVVKNAIVLVDFTNIIRARGVELRKAIIYSGRN
                     RLRPVLMTTFTTLLGLIPLAISTGDGSEVWRPLGISTIGGLFFSILITLVLVPVLYSV
                     FETRFKKKKKKID"
     gene            812..934
                     /locus_tag="VF870_15760"
     CDS             812..934
                     /locus_tag="VF870_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253702.1"
                     /translation="MLTVIKDDLVDPLLEKVKEINKEAEEQGIQAFVWNVEKMF"
     gene            complement(1012..1536)
                     /locus_tag="VF870_15765"
     CDS             complement(1012..1536)
                     /locus_tag="VF870_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253703.1"
                     /translation="MKDKKIELYFNSEDFKEKMKELEENLKNMPVPPNPPDVDIDFQI
                     DMDAFKEGMKNFKESFKNFDIKIDSSEFDMNELRSNMKELKKNMKGIKIEMHDLKGEM
                     KKLDSFLDELKSELVKDGYIKSENEKFDLEMNAAFTKVNDRVVKPEHQKKYLELYKSY
                     FDKEIEGTIKIDKD"
BASE COUNT          517 a          276 c          283 g          618 t
ORIGIN      
        1 cggaagatct attggagagg ttgcaggcga cataaaaaat tttgtttcaa gaatcgaact
       61 tccgccaaat acaactattg aatttaccgg gcaagttgaa cagcaaacag atgcatttta
      121 aagatttact gcttcttctc ggattaagta ttattctggt ttacatggta atggccgcac
      181 aatttgaatc tctgcttgat ccgtttataa ttatgttttc tgttccgttt gcattttcag
      241 gagtgttcat cgggttgttt gtaaccggaa cagctttcag cgttattgca tttcttggaa
      301 gcgtaatact tgttggtata gtagttaaga atgcaattgt acttgttgat tttacaaaca
      361 ttatcagggc aagaggtgtt gaactaagga aagcaattat ttattcagga aggaacagac
      421 ttcgtccagt gttgatgaca acattcacaa cgttgcttgg tttaattcca cttgcaataa
      481 gcacaggcga cggttcggaa gtgtggcgac cacttggtat ttctaccatt ggcggattgt
      541 ttttctcaat cctaataacg cttgttttgg tacctgtgct gtattcagta tttgaaacaa
      601 gatttaaaaa gaaaaagaaa aaaattgatt agaagatttt tatgaaagca gtaatgatag
      661 tttataatca tggtattact gaggaagtta atgaagcctt ggaaaaattg gaaattcggg
      721 gttatacaaa attcctgaat gttcacggac gaggtagtta aaaagatgat ccacatttgg
      781 gacacatatc tggccaagcc agaatgcggt tgtgttaaca gtaataaaag atgatctcgt
      841 tgatccattg cttgaaaaag taaaagagat taataaagaa gccgaagaac aaggcattca
      901 ggcttttgtt tggaatgtcg aaaagatgtt ttaaatattt ctgactggct aacctaaaaa
      961 gcctgcaaat tactctgcag gctttagtgg aggagagata attttatata actaatcttt
     1021 atctatttta atagttcctt ctatttcctt atcaaaataa cttttgtaca attctaaata
     1081 tttcttttga tgttccggtt taacaactct gtcatttact tttgtaaatg cagcattcat
     1141 ttcaagatca aatttttcat tttctgattt tatatagcca tctttaacaa gttcagactt
     1201 gagttcatcc aaaaacgaat ctaatttttt catttcacct ttcaggtcgt gcatttctat
     1261 tttaattcct ttcatgttct tctttaactc tttcatattg ctgcgcaatt cgttcatatc
     1321 aaactcagaa ctgtcaattt taatatcaaa attcttaaaa gattctttaa aatttttcat
     1381 accctcttta aatgcatcca tgtctatttg aaagtcgata tcaacatccg gcggatttgg
     1441 gggaacaggc atattcttta agttttcttc cagctctttc attttttctt taaagtcttc
     1501 tgaattaaag tatagttcta tctttttatc tttcaagtgt tttaaacttt catccagttt
     1561 ttacatgttt ttttcaaatt catcttcatc aaactcaagt ttaaagtgca tgaatttgtc
     1621 attatcaaaa tcatttttaa actttttcat atcatcacca aacttatcca tatcaaagta
     1681 gaacttgtga acat
//
LOCUS       DASWBY010000871         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_74399, whole genome shotgun sequence.
ACCESSION   DASWBY010000871 DASWBY010000000
VERSION     DASWBY010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74399"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VF870_15770"
     CDS             <1..582
                     /locus_tag="VF870_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9253704.1"
                     /translation="DIILLAGDIVDDKAKVLDERRIGESFTKLKSKYGVYSINGNHEF
                     INGVDSCVRYAEKFGIKFLRDGYELIDSSFYIIGREDSSMPQFTGTQRKSLEEIVKNI
                     PANYPKILLDHTPFKLEQAQQNNIDLQLSGHTHHGQIWPANLITKMIYEISWGYKKKG
                     NTHYYVSSGAGTWGPPVRTGSSSEIVNIKVKFE"
     gene            complement(628..1542)
                     /locus_tag="VF870_15775"
     CDS             complement(628..1542)
                     /locus_tag="VF870_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253705.1"
                     /translation="MYYYRLLIITLAMLISIPVYSQDDSTKTKDDWEWHWDDMDDWEN
                     WVVDYGFKKSRPAISLQYGLAEMSRTDIQSQFVDPALLELKLGYIKERSAWQTDNIIK
                     HSYKYLYLSNESNKLAGKEATGTEIESDMWRFGVGWSSGYGYKLGENAAIIPYYSYTL
                     NWSNINFMDSDVLTLVPDPDVEKLNLYDESFRFGTSAEGGVRVKLIENLMLDAGYERS
                     IVFQRHLFWKWAGSALIEVAAQGLLDSFINEVFKSSPAAGPVVNFLLKNALAYGIYEL
                     RQEKMNWPFPSEPPIAYDQFKFGMTFVF"
BASE COUNT          570 a          326 c          294 g          515 t
ORIGIN      
        1 gatataattt tacttgccgg tgatatcgta gatgacaaag caaaggttct ggatgaacgg
       61 agaatcggag aatcatttac aaaattaaaa tctaaatacg gtgtttattc aataaatggt
      121 aatcacgaat ttataaacgg agttgattcc tgtgtaagat atgctgagaa gtttgggata
      181 aaatttttac gcgatggata tgaattaatc gacagcagtt tttatataat tggtagagaa
      241 gacagctcaa tgcctcagtt tacaggcacg caaagaaaat cattagaaga aattgtaaag
      301 aatattccgg cgaactatcc aaagatttta ttagaccaca cgccatttaa attggagcag
      361 gctcagcaaa acaatattga tctgcagcta tcgggtcata ctcatcacgg acaaatatgg
      421 ccggccaact taattacaaa aatgatatat gaaattagct ggggctataa gaaaaaaggg
      481 aacacacatt actacgtttc atccggtgca ggtacctggg gaccccctgt aagaacggga
      541 agcagttctg agatagtaaa tatcaaagtg aaatttgagt aattgaaata aaaaacccaa
      601 ttcatttatt gaattgggtt tgcaaattta gaatacgaaa gtcattccaa acttaaactg
      661 atcgtaagca attggcggtt cacttgggaa tggccagttc attttctcct gtctaagttc
      721 ataaattcca taagccaaag catttttaag caggaagttt acaaccggac ctgccgcagg
      781 tgatgattta aaaacttcat taataaaact gtctaacaat ccctgtgctg caacttcaat
      841 taatgcactg cctgcccatt tccagaacag gtgccgctga aatacaatcg atctctcata
      901 accggcatcc agcataaggt tttcgattag tttaactcta acaccgcctt cagcgcttgt
      961 accaaatctg aaagattcat cgtacagatt taatttttca acgtcaggat cagggacaag
     1021 agtcagcaca tccgagtcca taaagtttat gttagaccaa tttaacgtat aggaatagta
     1081 tggaataatt gcagcgtttt caccaagctt atatccatat ccgcttgacc atccaacacc
     1141 gaaacgccac atatcagatt caatctcagt tcctgtagct tcttttcctg caagcttgtt
     1201 ggactcatta ctcagatata aatatttgta gctgtgtttt attatgttat ctgtctgcca
     1261 ggcacttcgc tctttaatat agcctaattt taattccaac agagccggat caacaaactg
     1321 cgattgaata tccgttctgc tcatctcagc taatccatac tgaagtgaaa ttgccggtct
     1381 tgacttttta aatccataat ctactaccca gttttcccag tcatccatat cgtcccaatg
     1441 ccattcccag tcatcctttg ttttagtgga atcatcctga gagtaaacag ggattgagat
     1501 aagcatagct aaagtgataa ttagtaacct atagtaatac atttgacctc ctgtataaaa
     1561 atatcatttg aatagtctat gaataatgaa tcatttattt gctttactta aaacttgttc
     1621 atttacccat ctaaaaatat taaacgataa tctcatttaa cagtaattta taataactaa
     1681 tgaaatgtac tgataaatgg taatt
//
LOCUS       DASWBY010000872         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_332170, whole genome shotgun sequence.
ACCESSION   DASWBY010000872 DASWBY010000000
VERSION     DASWBY010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332170"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VF870_15780"
     CDS             <1..1104
                     /locus_tag="VF870_15780"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX9253706.1"
                     /translation="GSAADANNGFRVQMRFPYNGGWSPWLTVGYWKTNILGSYSKPTY
                     GGGYIDYDNVKLNSYQNSWQFKVIMKRNSASVESPTIHKLSFFVSDSKTTDSVNINSI
                     VNDSPAAVFIPTTFIYQYGVDPGIGGDICSPTSVAMILKSYNIDVDPRQFALDNYDTN
                     FGMFGIWPRVVQNAAEYGLDGAVTRYRTWSEAREVLANGGRISMSVGPPLYTGHLMML
                     AGFTSDGRPIVHDPAKSNGYSYIFNKTLLSQSWFNKGGVAYTFYFPDSATVNVENSNP
                     NIIADDYQLLQNYPNPFNPSTSISFSLPQAGLIKLTVYDILGNEIEVLVNDFLNAGIY
                     TTTFNAANLSSGVYIYRIQTENFVKAKQMILLK"
     gene            complement(1216..>1979)
                     /locus_tag="VF870_15785"
     CDS             complement(1216..>1979)
                     /locus_tag="VF870_15785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253707.1"
                     /translation="WQTASGQDANSVSKEVFFVSTTDLHLTGSSNGDFELAGTPIAGF
                     TTDIDGDTRSTTDPYKGADEASLPVPVELVAFNASLVQGDVVLTWKTATEINNRGFEI
                     ERGTNGEFATIGFISGSGTSTQEQSYTFIDKEVAVGIHTYRLKQIDFDGSYSYSNIIE
                     VDVTTPLTFSLSQNYPNPFNPTTKISYSVPFDSKVIISVYSVTGELVMELVNDNVSAG
                     SYSVDFNGSNLASGMYIYKMTAGSFTQSNKMMLMK"
BASE COUNT          621 a          345 c          360 g          653 t
ORIGIN      
        1 ggaagtgcgg ccgacgctaa taatggattt agagttcaga tgcgatttcc atataacggc
       61 ggttggtcac cctggttaac tgttggatac tggaaaacca atatattagg tagttattca
      121 aaaccaactt acggcggcgg atatatagat tatgataatg taaaattaaa ttcttatcaa
      181 aactcatggc aatttaaagt aattatgaag cgtaattccg cttctgttga gtcgccaact
      241 atacataaat taagtttttt tgtcagcgat agtaaaacaa ctgattcagt taatatcaat
      301 agcattgtca atgattctcc tgctgcggtt ttcattccca caacttttat ttatcaatat
      361 ggtgttgatc caggaattgg cggtgacata tgttctccaa cttcagttgc gatgatatta
      421 aaaagctata atattgatgt tgatccgcgc cagtttgcac ttgataatta cgatacaaat
      481 tttgggatgt ttggtatctg gccaagagtt gtgcaaaatg ctgctgagta tggattagat
      541 ggtgctgtta cccgttaccg aacctggagt gaagcaaggg aagtgcttgc taatggaggc
      601 agaatctcta tgtcagtcgg cccaccactt tacacaggtc atttaatgat gttagctgga
      661 tttacttcag atggcagacc gatagttcat gatcctgcaa aatcaaacgg ttattcttat
      721 atatttaata aaacgttatt atcgcaatcc tggtttaata aaggcggagt tgcatataca
      781 ttttatttcc cagacagcgc tactgtaaat gtagaaaatt caaacccaaa tattattgca
      841 gacgattatc agcttttaca aaattatcct aatccattta atcctagtac atcaatttct
      901 ttctcacttc cgcaagccgg attgattaaa ttaaccgtct atgatattct tggaaatgaa
      961 attgaagttt tagtaaatga ttttcttaac gcaggcatat acacaactac atttaatgct
     1021 gctaatttat caagcggtgt ttatatttat agaatccaaa ctgaaaattt tgttaaagct
     1081 aaacagatga tattgttaaa ataaaatact tatcgagttt agatgaatta ttttcatata
     1141 agcgttgaaa ggttagaaat aaaaaaagtc ccgacgaatc gggacttttt tataactaat
     1201 ttgaagttaa gaatattact tcatcaacat cattttgttg gattgagtaa agctgcctgc
     1261 agtcattttg taaatgtaca taccgcttgc aagattactt ccattgaaat caactgaata
     1321 ggaacctgct gaaacattat catttactaa ttccattacc aattcacctg taactgaata
     1381 aacagaaata attaccttgg aatcaaatgg aacgctgtaa cttatctttg ttgtaggatt
     1441 gaatgggttt ggatagttct gacttaaact gaatgttaat ggtgttgtta catcaacttc
     1501 aataatattt gagtatgagt aactgccatc aaaatcaatt tgttttagtc tgtaagtatg
     1561 tataccaaca gccacttctt tatcaataaa tgtataggat tgttcttgtg tgcttgtacc
     1621 tgagccggaa atgaatccta tagttgcaaa ttcaccattt gttcctcttt caatctcaaa
     1681 tccacgattg tttatttccg ttgcagtttt ccaggtcaat acaacatcac cttgaacgag
     1741 tgaagcatta aatgcaacta attcaacagg tactggaaga gaagcttcat ccgcaccttt
     1801 gtatggatct gttgtgcttc tagtatcgcc atcaatatcg gttgtgaaac cagcaattgg
     1861 agtaccggca agctcgaaat ctccatttga ggagcctgtc aagtgtaagt ctgtagttga
     1921 tacaaaaaag acttcttttg acacagagtt cgcatcctgt ccggaagctg tctgccaat
//
LOCUS       DASWBY010000873         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_563169, whole genome shotgun sequence.
ACCESSION   DASWBY010000873 DASWBY010000000
VERSION     DASWBY010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563169"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VF870_15790"
     CDS             <1..788
                     /locus_tag="VF870_15790"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9253708.1"
                     /translation="SENKLTTLKVKIMKTQNKNITFFQFGFIVLLYMFFIAGYNYAQV
                     DIKSLENSVRKTIASYYDQQFNISADATGQVTISGKVNTLFDKLKIGELISQVNQVTG
                     IKNKLEILVDEPTADGIIKANIENEYQLNNTILEPEKIIVSVTNGIVNLSGTVSYFRE
                     KLMAQSIASWQDGVIDMTSSIKVLPHKLIVSDQNLKDLISDIMNKHFLIENNIKYDVN
                     NGVVTLYGTAKNLYAIDHLQEEIQHLLGVKEVINQLTLSKNEE"
     gene            810..1610
                     /locus_tag="VF870_15795"
     CDS             810..1610
                     /locus_tag="VF870_15795"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX9253709.1"
                     /translation="MKKINAFIKAACFSYIILLMSLSPGCASSQEEINNASNQAYNVA
                     DLNSYGEWINTDNYGSVWQPYAVIGWMPFDNGHWAYANENWTWVSYEPFGWIVYHYGY
                     WYDDPLYGWVWIPSDGIWSPANVSWFQYGDYIGWAPLGPRGIDYGNPFDRDHDKYWHI
                     VRSEDFTRDNIRDFRIKDPVKNENSKEIIYKQPDRKFIEKLSGKLVNEVKMQHENVTL
                     PPRQIQKMNLPPQEHQRVEQNSQRVRKEVLVPKEEFHRQQSERMKKQK"
     gene            1996..>2363
                     /locus_tag="VF870_15800"
     CDS             1996..>2363
                     /locus_tag="VF870_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9253710.1"
                     /translation="MNKKNLGNGTLKISALGLGCMGMSEFYGDTNDEESIKTIHRALE
                     LGINFFDTADMYGVGKNEILVGKAMKGKRDQFILATKFGNVRGNDGSFLGVNGKPEYV
                     KQACEASLKRMENDVIDLYY"
BASE COUNT          868 a          320 c          454 g          721 t
ORIGIN      
        1 taagcgaaaa caaattaaca actttgaaag tgaagatcat gaaaacgcaa aataaaaata
       61 ttactttctt tcaatttggg tttatagtcc ttttgtatat gttttttatt gctggataca
      121 actatgcgca ggtcgacatt aagtcacttg aaaattctgt tagaaaaact atcgcaagtt
      181 attatgacca acagtttaat atttctgcgg acgcaaccgg acaagttact ataagtggta
      241 aagtaaacac tttgttcgat aaacttaaaa ttggggaatt aatatcccaa gttaatcaag
      301 taacaggaat aaaaaataaa cttgagatcc tggtagatga acctacagca gatggaataa
      361 ttaaagcgaa cattgaaaat gaatatcaat tgaacaatac aatacttgaa cctgaaaaaa
      421 ttattgtaag tgtgactaat ggtatagtaa atctttcagg taccgttagt tattttcgtg
      481 aaaaacttat ggcgcagtca atcgcatcat ggcaggatgg agtgattgat atgacaagca
      541 gtataaaggt gttaccccat aagctaattg taagcgatca aaatttaaaa gatcttattt
      601 cggatattat gaataaacat tttttaatag aaaataatat caagtatgat gtcaacaatg
      661 gtgtagtgac tctttacggt actgcaaaaa atttatatgc tatagatcat ttgcaggaag
      721 agattcaaca cttgcttggt gtaaaagagg ttatcaatca attaacatta agtaagaatg
      781 aagaatagat tagaaaaaag gagttcgaaa tgaaaaagat aaatgctttt atcaaagcag
      841 catgtttttc ttatataatt ttactaatgt ctttatcacc gggttgtgct tccagccagg
      901 aagaaattaa taatgcatca aatcaagcat ataatgtagc ggatctaaat agttacggtg
      961 aatggataaa tactgacaat tacggcagtg tatggcagcc ttatgcggtt attgggtgga
     1021 tgccttttga caatggtcat tgggcgtatg ctaacgaaaa ctggacgtgg gtttcttatg
     1081 aaccattcgg ctggattgta tatcattacg gctactggta cgatgatccg ctttatggat
     1141 gggtctggat tccttcggat ggtatttggt caccagctaa cgtatcatgg tttcaatatg
     1201 gtgactacat tggttgggca cctttaggtc cacgaggtat tgattacgga aatccttttg
     1261 atagagatca tgataaatat tggcatattg taagaagtga agattttacc agagataata
     1321 ttcgtgattt tagaatcaag gatccggtta aaaatgaaaa cagcaaagaa ataatttata
     1381 agcagcctga tcggaaattt attgaaaaac taagtggcaa actggtaaat gaagttaaaa
     1441 tgcagcacga aaatgtgaca ttacctccac gacaaattca aaaaatgaat ttaccaccgc
     1501 aagaacatca aagagtagaa caaaattcac agagggttcg aaaggaagtt ttggttccaa
     1561 aagaggaatt tcatagacaa caaagcgaga ggatgaaaaa gcaaaaataa aaataattag
     1621 ccttttatat tcgtttttta ataagagacc atttctattg atttttggaa agtagattag
     1681 tattagaaat ttttcatgat aatttttctt tctataaatg tcattgagat tgtctcataa
     1741 ttcgagtttt atttctcatt aatgtgaaag cctgcctcca ctggcaggca tgtgggaatc
     1801 cataataaat aaaatagatt cgtaaattct cacgaagaaa tgcctatgac agtgctaata
     1861 attatttatt tttaattttt aaaacagcct tcaaaatact tttccactcc tgtatatttt
     1921 attaaaacta tatttaaatt ttagttatta gaaaagatat atctcaaaac attataaaaa
     1981 gaagaggtca taattatgaa caaaaagaat ttaggtaacg gaacattaaa aatatccgct
     2041 ttaggcttgg gctgcatggg aatgtctgaa ttttacgggg atactaatga tgaagaatca
     2101 ataaaaacaa ttcatcgtgc attggaactg ggaataaatt ttttcgatac agcagatatg
     2161 tatggtgttg gtaaaaatga aatattagtc ggcaaagcaa tgaaagggaa gagggatcaa
     2221 ttcattcttg caacaaagtt cggaaatgtt cgcggcaatg acggcagctt tttaggagta
     2281 aacggaaaac cggaatacgt taaacaagcc tgtgaagcaa gtttgaagag aatggaaaat
     2341 gatgttatcg atttatatta tca
//
LOCUS       DASWBY010000874         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_671950, whole genome shotgun sequence.
ACCESSION   DASWBY010000874 DASWBY010000000
VERSION     DASWBY010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671950"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(607..915)
                     /locus_tag="VF870_15805"
     CDS             complement(607..915)
                     /locus_tag="VF870_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253711.1"
                     /translation="MSVFESSIKNHRYDPIKINPIIKYGIKIYIKGNDWDLISRVRPA
                     INICEIIIRRMTTLKLSNFDPKTIIYLSDRKTKSIENVEKRVINNSDPFVRISNSVRM
                     "
BASE COUNT          526 a          389 c          258 g          548 t
ORIGIN      
        1 cattgatccg cagaaagctt gtatagatat ttcagatttt aaacatgaag agatgtataa
       61 tagtattgtt ccgccacctc caccgccacc ttttgttcag caacataaat ttatcccccc
      121 gataagtaaa taaaaaacac aaatgctaac acggacggta taattaataa gctcgtcatg
      181 gtttttgctt ttctcctact ttcttgacag aaaacaatac cctgaccaag cctcgcgccc
      241 caatctcgca ggctcgacat agggcgctcc cactggttaa ttcttttaag ttactgcatc
      301 aggatcatca actaactccc agacacattg taccgcgaaa cgcctatagg caagacaaat
      361 tgcacatgga aggcaaaaac cacgccgtgc ttcgttcaat cggcctcatg ggcattatgc
      421 caaaaaacct ggacactacc ttccggcctc ctttcactca gcaacgctta ctaaattata
      481 ccgccgggcc gttaggcgta agtaacaaac aaagaccatt atactaccat agatgaaaaa
      541 acataatttc tattcaatac tcggactagt atccacaggc attatactga ttagttatct
      601 tatttattac attctcactg aatttgatat tcttacaaat ggatcgctgt tattgataac
      661 ccttttttca acattttcaa tactttttgt ttttctatca ctcagatata taatcgtttt
      721 tggatcaaaa ttggacaatt tcaaagttgt catcctcctg atgattattt cacagatatt
      781 aatcgcaggt ctaactctgc tgattaagtc ccaatcattc cccttaatat atatcttaat
      841 tccatattta attatcggat tgattttaat aggatcatac ctatggttct ttatactgct
      901 ttcaaaaact gacaaaacag aaattagagg attatcattt cttcaatact ttggaataac
      961 cctattactg ttagttatac ttagtgtaat atttttattt actactaaag gagaaaataa
     1021 aactttctta tcaattaatc aattgtttga agctgttcct tatatctttt tagttttatt
     1081 ttttaccaaa catattaatc atcctactga taaattaaaa aatttaccta acgcctaaca
     1141 cgaacaataa agttcatttg tttgtcacac tttttgcaat ttgcttctac taccaagaca
     1201 ggtttgtaat aacacaaacc gcgaccgcgc caatgcttcc aatatagctt cgtatacctt
     1261 gacttgtgac ctgctctcaa taaaacttgt accgcgacac tagtaaaact cacttagttt
     1321 tgtaccatga tggcaaaaaa tgcgccatgc ttcgttcatt acggctggta ccaggactct
     1381 accacgattc ggatttcctg ctccagcctg cattcactca gcaacacaaa cgcactttat
     1441 tgccgggccg ttagcaaaaa tgcttgcagc ggtttgaaat ggcctcgcgc cgtacccaga
     1501 tagtattctg atgacactaa aattctcttg tatccagaca gaaaacaccg acactccggt
     1561 ggctgcggga cagatttgtt tgaaatttaa attaactggt acattgacag atattgtcgc
     1621 cggccatttc ttttatcctg actaatctgt atcgcacttt tgctaacggc acagattaaa
     1681 attttatgga aatcctacgt tcctgcagca aaaatgattg g
//
LOCUS       DASWBY010000875         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_111968, whole genome shotgun sequence.
ACCESSION   DASWBY010000875 DASWBY010000000
VERSION     DASWBY010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111968"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..1328)
                     /locus_tag="VF870_15810"
     CDS             complement(285..1328)
                     /locus_tag="VF870_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX9253712.1"
                     /translation="MIKSKFRLPAEWEYHEATWIGWPNNKQDWPGKFQPIPWVYGEIV
                     RYLSRGEKVRVIVQSKDHQKKAEQVLKSVDADFNNVEFYKLETDRNWLRDAGPQFVKN
                     QKNETVLINFKFNAWAKYDNYKLDAKIPKMISKKLKLDLIKAKHQNREVVLEGGSIDY
                     NGLGTIITTEECLMDNEIQVRNKNFAKKDYENVFNKYLGTTNVIWLNKGIAGDDTHGH
                     VDDITRFVSPKTVVTVIESHPNDPNYIPLMENREILEGAKLEDGSGLEIIELPMPSPV
                     IFKGERLPASYANFYISNYAVLVPTFNDPNDRVALGILSELFTDRPIIGIHAVDLVWG
                     LGTLHCLTHEQVL"
     gene            complement(1325..>1847)
                     /locus_tag="VF870_15815"
     CDS             complement(1325..>1847)
                     /locus_tag="VF870_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9253713.1"
                     /translation="DDPSYYEKYYFTPGDLGFKSFDTEFGNIGILICWDQWYPEGARL
                     TALQGASILFYPTAIGWHPYEKKEHGKPQFESWQTIQRSHAIANGVYVASVNRIGLEK
                     ETKDSAGIEFWGKSFICDPQGIIIAEASHDKEEIIIAEVDLDRIEYIRRNWPFLRDRR
                     IDAYGDITKRFLK"
BASE COUNT          556 a          361 c          289 g          641 t
ORIGIN      
        1 atccgattat tgcgttaatg cttggctgga tattcttaaa tgagcagtta aatattttta
       61 ttcttcttgc atcagttgtg ataatagccg gtgttgtact tgttaagatt ggaagcaaca
      121 aaagaacgct tcttaaaaga ttttaatagt taagacgcct taattttgga ttttgccttg
      181 tgtcattccc ctgaaaacgg gaagccaaag gctcttaata aaagattccc actttcgtag
      241 gaatgacaaa ctaattaaaa caaattatga attagaatta gtaattataa aacctgttcg
      301 tgtgttaaac aatgtaatgt tccaagtccc catactaaat caacagcgtg aatcccaatt
      361 ataggtctat cagtaaataa ttcggataaa ataccaagag ctactctgtc gtttggatca
      421 ttaaatgtag gaacaagtac tgcataattt gagatataaa aattagcata acttgccgga
      481 agtctttcgc ctttaaaaat aacaggagaa ggcattggca gttcaataat ctctaaacca
      541 gaaccatctt ctaactttgc accttcaaga atttccctgt tctccattaa cggaatatag
      601 ttgggatcat tcggatgtga ttcaataact gtcacaacag tttttggact tacgaatcta
      661 gttatatcgt caacgtgtcc gtgagtatca tctccggcaa ttcctttgtt taaccagatt
      721 acatttgtag taccaagata tttattaaaa acattttcat aatctttctt agcaaaattt
      781 ttatttcgga cttgtatttc attatccatc aaacattctt cagtagtaat aattgttcca
      841 agtccattgt aatcaatgct tccgccttca agtactactt ccctattttg atgttttgct
      901 tttattagat caagtttaag ctttttagaa atcatttttg gaatttttgc atccagtttg
      961 taattatcat acttagccca ggcattaaac ttaaagttga taagcacagt ttcattcttt
     1021 tgattcttaa caaattgtgg tccagcatcc cgcagccaat ttctatcagt ttcaagtttg
     1081 taaaattcaa cattgttaaa atctgcatct acagatttta aaacttgttc agcttttttt
     1141 tgatgatctt ttgattgaac aatgactcta actttttcac cacgagatag gtaccgtaca
     1201 atctcaccat acacccaggg aataggctga aactttcccg gccaatcctg cttgttattt
     1261 ggccagccaa tccatgttgc ttcgtgatat tcccattctg cggggaggcg aaattttgat
     1321 tttatcattt taaaaatctc ttagttatat caccataagc atcaattctt ctgtcacgca
     1381 agaaaggcca attgcgccga atgtattcaa ttctatcaag atctacttct gcaataatta
     1441 tttcttcttt atcgtgtgat gcttcagcaa ttataattcc ctgaggatcg cagataaaac
     1501 tctttcccca aaattctatt cctgctgaat ctttagtctc tttttccaaa ccgattctgt
     1561 ttacagatgc aacataaact ccatttgcaa tagcgtgcga gcgctgaatt gtctgccagc
     1621 tttcaaactg cggtttaccg tgttctttct tttcgtatgg atgccagccg attgcagttg
     1681 gataaaataa aatactggca ccttgcaacg ctgtcaatct agcgccttca ggataccatt
     1741 gatcccagca gattaatatt ccgatgtttc caaattctgt atcgaaagat ttaaatccta
     1801 aatcaccggg agtgaaataa tatttttcat aataagatgg atcatca
//
LOCUS       DASWBY010000876         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_600513, whole genome shotgun sequence.
ACCESSION   DASWBY010000876 DASWBY010000000
VERSION     DASWBY010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600513"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VF870_15820"
     CDS             <1..293
                     /locus_tag="VF870_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253714.1"
                     /translation="KNFPYKIDEAIKRTDELIKWLYKHQSQQQRKHLKNRLFVALYSK
                     DGEHWKLKSEITWLKDRIEKYMLGFNPNYLLKFNFEKDKETRSDVIWAIRDK"
     gene            497..1192
                     /locus_tag="VF870_15825"
     CDS             497..1192
                     /locus_tag="VF870_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253715.1"
                     /translation="MKKLLLISITLIISITFISCSDSSKDKSDKSGKESTSEFQKRYG
                     IKSGVIEYIITGSQEGTKTLYFDNWGMRQAEYTRSILSVSGFTKPLNLVNIIDGEYQY
                     MINIDQNSGTKTRNPILKSIEQLKGQKGFNEFGEQMLLSMGANKIGSESFLGKDCDVY
                     EMKNTGTKLWVWEWLTLKSETKSGGLNINLTATRINEGGSVPTEKFRIPEKVVLNEVD
                     IDNIENEMREENK"
     gene            1189..2553
                     /locus_tag="VF870_15830"
     CDS             1189..2553
                     /locus_tag="VF870_15830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9253716.1"
                     /translation="MISFLPIGGGNEIGANSYYLNFNGNGIILDCGIHPQKTGLESLP
                     NFDLIKDKSVETCLVSHAHQDHIGSLLFLVKRFPYVKIITTPQTRALAELTLHNFVSI
                     LKKEIEEDDFEFYSHDEVDLLIQMIEYKEYQNQFELNSYHQQKDAKVNCTFYDAGHIL
                     GSAGILLENNNHKIFYTGDINLSSQTLQPAAQLPETKIDTLILETTYGATDSELLLDW
                     NSESLRFAKEANSILNNGGSILIPVFSLGKMQEMLGTIWQLMLKRKLTTVDVFTGGLG
                     TKINRAYDYNRFVVKRVDSEFELNTIQQKDYYEVSNPEDFFKHPCIVLASSGMMIKGT
                     SSFTFAKRWLKQKDSAIFTVGYINTKTPGYVIANAERGDKIQLTGFDELIEVNCTIKN
                     FKFSAHARREDLLKIVDKLRPDNVILTHGDEDALDWIGSSILKKYKNIKIQTAESRKE
                     LKFF"
     gene            2671..>3362
                     /locus_tag="VF870_15835"
     CDS             2671..>3362
                     /locus_tag="VF870_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX9253717.1"
                     /translation="MSRLKKQVQDKIEAWRPRTTRLLKEFGNVKVDEVDIGQVIGGAR
                     DIKSLVTDISYLDPFEGIRFRGFTIPEVLEKLPKVPGGEMPYVEGFFFFLLTGDIPSM
                     EDVQEVAEEFRKRSAVPKYVFDILKTMPADSHPMVMFSTAILAMQRESEFVKEYNTGK
                     LKKNDYWAPTYEDGLNLLAKLPGIAAFIYRLKYKNGELIDSDPKLDLGGNFAHMMGIA
                     KPYDDVARMYFIL"
BASE COUNT         1213 a          515 c          623 g         1011 t
ORIGIN      
        1 ctaaaaactt tccgtataaa attgatgaag caataaagag aacagatgaa ttaattaaat
       61 ggctgtacaa acatcagtct caacagcaaa gaaaacattt aaagaacagg ttatttgtag
      121 ctctttattc aaaagatggc gaacattgga agttgaaatc agaaattacc tggctgaaag
      181 atagaattga gaaatatatg ctagggttta atccaaatta tttattaaaa tttaattttg
      241 aaaaagataa agaaacacgg tctgatgtaa tttgggcgat aagagacaaa taaaatttgc
      301 accttttcat cctgagcaaa gtcgaaggat gaaaattgaa atataacaga gtgtcacact
      361 tcgacttcgc taggtgtgac aagctaattt accacgacaa aattgacaat aaatttttat
      421 tacttctaaa ttttaattga agtaagattg cactttactt attttcaatt actgaaaaac
      481 ataaagaaat aagacaatga aaaaattatt attaatttca attactttga tcatctcaat
      541 aacttttata agctgtagtg atagcagcaa agataaatct gataagtcag gcaaggaatc
      601 tacgtctgaa tttcagaaga gatatggaat taaatctggc gtgattgaat acataattac
      661 aggctcgcag gaaggaacaa aaactcttta cttcgataac tggggaatga gacaagctga
      721 atacacaagg tctatcttaa gtgtaagcgg atttacaaaa cctttaaatc ttgtaaatat
      781 tattgatggc gaatatcagt atatgattaa tattgatcag aacagcggaa ctaaaacaag
      841 aaatccgata ttaaaatcta tagaacagct caagggccaa aaaggattta acgaattcgg
      901 ggagcaaatg ctgcttagca tgggcgcaaa taaaattggt tctgaatctt ttcttggtaa
      961 agattgcgat gtttatgaaa tgaaaaacac aggcacaaaa ctttgggttt gggaatggct
     1021 gactttaaaa tctgaaacca aatcaggggg attaaatatc aatcttacag caacaagaat
     1081 taatgaaggc gggtctgttc ccacggaaaa atttaggatc cccgaaaaag tagttttaaa
     1141 tgaagttgat attgacaaca ttgaaaacga aatgagagaa gaaaataagt gatttctttt
     1201 cttccgattg gcggtggaaa tgaaattggt gcaaacagct actacttaaa ttttaacggc
     1261 aacggaatta ttcttgattg cggtattcat cctcagaaaa caggactaga atctcttcca
     1321 aactttgatt taattaaaga taaatcggtt gaaacctgcc tggtttctca cgctcatcag
     1381 gatcatatag gctcacttct ctttcttgta aaaagatttc cctacgtaaa gatcataaca
     1441 actccccaaa ccagagcact tgcagaactt acgctacaca acttcgtatc aattctgaaa
     1501 aaagaaatag aagaagatga ttttgaattt tattcacatg atgaagttga tttgctaatc
     1561 cagatgattg agtacaagga atatcaaaat caatttgaac ttaattctta tcatcagcaa
     1621 aaagatgcaa aagtaaattg cactttttat gatgcagggc atatacttgg ttcggcggga
     1681 attcttttag agaataataa tcataaaata ttttatactg gagacattaa tctttcttca
     1741 caaacacttc aacccgcggc acaacttcct gaaactaaga tcgatacttt gattcttgaa
     1801 acaacttacg gggcaacaga ttctgaatta ctgttggatt ggaattcaga atcactgcgt
     1861 tttgcaaaag aggcaaatag tattttaaat aacggcggtt ccattctaat tccagttttt
     1921 tctctaggta agatgcagga aatgcttggc acaatctggc agttgatgct aaaacgaaaa
     1981 cttacgacgg tagatgtttt tacaggtgga ctcggcacaa aaataaaccg tgcttatgat
     2041 tacaatcgtt ttgttgttaa gagagttgat agtgagtttg aattgaacac aattcaacaa
     2101 aaagattatt atgaagtttc taatccggaa gacttcttta aacatccctg catcgttttg
     2161 gcatctagcg gaatgatgat taaaggcaca tcatctttta catttgcaaa aagatggttg
     2221 aagcaaaaag attctgcaat ctttactgtt ggatacataa atactaaaac tcctggttat
     2281 gtgattgcaa atgctgaacg tggagataaa atccagctaa ctggttttga tgagctaatt
     2341 gaagttaact gcacaattaa aaactttaaa ttttctgcac acgcaagaag agaagacttg
     2401 ttaaagattg ttgataaatt aaggcctgat aatgttattt taacacacgg ggatgaagat
     2461 gccttggatt ggataggtag ttcaattctt aaaaagtata aaaacataaa aattcaaact
     2521 gccgagtcga gaaaagagtt aaaatttttt taattgccaa acgcaatagt taaattttct
     2581 ttgtatatta aaatcaaata aaattcattt tcctttttta atagaaggaa ataaaaatag
     2641 cacaatttat atttatctca ggaggagaaa atgtcccgct tgaaaaaaca agtccaggat
     2701 aagatagaag cctggcgtcc gcgcaccaca cgtctgctta aagaatttgg taatgtaaaa
     2761 gttgatgaag ttgacatcgg tcaagtaatt ggcggtgcaa gagacattaa gagtctggtt
     2821 acagatattt cttatcttga tccatttgaa ggaattcgtt ttcgcggatt tacaattcca
     2881 gaggttttag aaaaacttcc taaagtgcca ggtggagaaa tgccatatgt agaaggcttt
     2941 ttcttctttc tgcttaccgg cgatattcct tcaatggaag atgtgcagga agttgctgaa
     3001 gagtttagaa aacgatctgc agtaccaaaa tatgtttttg atattttaaa aactatgccc
     3061 gcagattcac atccaatggt tatgttctcg actgctattc ttgctatgca gcgggaatct
     3121 gaatttgtaa aagaatataa tacaggcaag cttaaaaaga atgattactg ggctccaact
     3181 tatgaagatg gtttaaatct tcttgcaaaa cttcccggta tagcggcatt catctataga
     3241 ttaaaatata agaatggtga attaatagac tcagatccta aacttgatct tggcggtaac
     3301 tttgctcata tgatgggcat tgcaaaacct tacgatgatg ttgcaagaat gtattttatt
     3361 ct
//
LOCUS       DASWBY010000877         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_371441, whole genome shotgun sequence.
ACCESSION   DASWBY010000877 DASWBY010000000
VERSION     DASWBY010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371441"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2039)
                     /locus_tag="VF870_15840"
     CDS             complement(<1..>2039)
                     /locus_tag="VF870_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX9253718.1"
                     /translation="NILKKIQTLLVIGLVILPTNIYAQSNDDCLMCHSDNELTMEKKG
                     KEISLFVNENVFKGSTHSKLSCVACHKGFDPESMPHAENIQEINCVSCHQKVLTQHSF
                     HPQILKSKGLGKTADTDCKNCHGIHDVVSPKDPKSKWSSKNLIVTCGNCHKNQSDSYL
                     TSNHAAAFKSGDKSAPNCITCHKSPITKIAFGDNKVEMKKAQEKLCLSCHLDDKDVRE
                     RITPSAGFIHAYDKSVHGYALNVKNNGDAANCINCHSAHDIIIGTDSRSTVYKLNIPN
                     TCGKCHTEIKNEYLESIHGQLVMKGNGDAPSCTNCHGEHNILNIKDPNSPVAFQNVSR
                     QVCSPCHESMKLSEKYGLAGNRYKTFSQSYHGLALSGGSASVANCGSCHGVHNIKPSS
                     DPTSMINKNNLVKTCGKCHPGANETFTQGNIHVTLDKADEPILYWISTTYIILLFSVL
                     SGMFLHNLLDLIKKSKIRKLKQLGEIAEEDHGHSLYLRMTVSERIQHATMAISFMILV
                     LTGFMLRYPDSWWVSHIRDLSSHVVEYRSWIHRIAAVAMILVSFYHIYYISFTKRGRE
                     LVKDLFPKLSDFTDAIGVAKYNLGISKDKPKLDRFSYVEKAEYWALVWGTIVMSLTGL
                     LMWIYIDYIGVFSKIDWDIARTIHYYEAWLAFLSIVIWHFYFVIFNPDIYPMSLA"
BASE COUNT          648 a          371 c          329 g          691 t
ORIGIN      
        1 caggcaagac tcataggata aatgtcagga ttgaatatta caaagtagaa atgccatata
       61 actatggaca ggaatgctaa ccaggcttca taatagtgaa ttgttctagc aatatcccaa
      121 tcaatttttg aaaagacacc aatgtaatcg atgtaaatcc acattaataa tcctgttaaa
      181 ctcattacaa tagtacccca aaccaaagcc caatattctg ctttttcaac ataactaaat
      241 ctgtctagct taggtttatc ttttgagatt ccaagattgt actttgcaac accaattgca
      301 tctgtaaaat cacttaattt tggaaacaga tcttttacta attctctacc tctttttgta
      361 aagctaatgt aatagatatg gtaaaaacta actaagatca ttgcaactgc tgcaatacga
      421 tgaatccaac ttctatattc aacaacatga ctggataaat ccctgatatg acttacccac
      481 caggaatcag gatatcttaa cataaatccg gttaacacta aaatcataaa gctaatagcc
      541 attgttgcgt gttggattct ttcactaact gtcattctaa gatataatga atgtccatga
      601 tcttcttctg caatctcacc aagctgtttt aattttctta ttttagattt tttaataaga
      661 tctaaaagat tgtgtaaaaa cattccgctg agtacagaga aaagcagtat tatatatgtt
      721 gtagaaatcc aataaagaat aggttcgtct gccttatcta aagtaacatg tatgttacct
      781 tgagtaaaag tttcgtttgc gccaggatga cactttccgc aagttttaac aagattattt
      841 ttattaatca tcgaagttgg atctgaagat ggtttaatat tatgtacacc gtggcaacta
      901 ccgcaatttg ctacagaagc agatccgccg cttaaagcaa ggccatgata tgactgagaa
      961 aaagttttat atcgatttcc ggccaagcca tatttttcag aaagtttcat agactcatgg
     1021 cagggagagc agacttgacg tgagacattt tggaatgcaa ctggtgaatt aggatcttta
     1081 atatttaata tattatgctc tccatgacaa tttgtacagg aaggggcatc accattgccc
     1141 ttcattacaa gttgtccgtg aatgctttct aaatactcat ttttaatttc tgtatgacat
     1201 ttaccgcacg tatttggaat atttaattta tatacagttg atctggaatc tgtacctata
     1261 ataatatcat gggcagaatg acaatttata caattagcag catccccatt attcttcaca
     1321 ttaagagcat atccgtgcac actcttatca tacgcatgaa taaagcctgc tgatggagtt
     1381 attcgttctc tgacatcttt atcatctaag tgacaggaaa gacaaagctt ttcctgtgct
     1441 tttttcattt caactttatt atcaccaaag gcaattttag ttatcggcga tttatgacaa
     1501 gtaatacaat tcggtgcgct tttatctcca cttttaaaag cagcagcatg attagaagtt
     1561 aaataactat cgctttgatt tttatggcag tttccgcagg ttacaataag gtttttacta
     1621 ctccatttgc ttttaggatc ctttggagaa acaacatcat gtattccatg acagttttta
     1681 caatcagtat ctgctgtttt tcccagtcct ttcgacttta aaatttgcgg gtgaaaagaa
     1741 tgttgtgtta gtactttttg atgacaactg acacaattaa tttcctggat attttccgca
     1801 tgcggcatgc tttcaggatc gaatccttta tgacaggcaa cgcagcttaa tttagagtga
     1861 gtcgaacctt taaaaacatt ttcattaaca aacagggaaa tttcttttcc cttcttttcc
     1921 atagtgagtt cattatcact atggcacatc aaacaatcat cattcgattg agcatatata
     1981 tttgtgggta gaataactaa tccgattaca aggagggttt gtattttttt taagatgtt
//
LOCUS       DASWBY010000878         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_74736, whole genome shotgun sequence.
ACCESSION   DASWBY010000878 DASWBY010000000
VERSION     DASWBY010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74736"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="VF870_15845"
     CDS             <1..1829
                     /locus_tag="VF870_15845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX9253719.1"
                     /translation="VQGYTIYVDEYTELRGPNNTTVIFSFFQLNDLVQVQADSRGDGT
                     YLAARVKSDDGAGGGNENEVELIGYVTEVGTSSFAINGTAFFVDVNTEYRGRHGNTFS
                     FEQIVPGILLEVKATLQTNGDLLATRVKTEDDENQHESELEITGLIESLGANSLTVGQ
                     QEFFADAQTIVLNHNNMPINFSDLNIGDKVEVKAYKQSDGSYLAVRIKLEDLPGNEIE
                     FTAQIESIVGPDVTINGIVFSTDSSTVFLDDNRMPVTIASLAVGMWVEVKGLKRQDGS
                     YYASRIQIEDFVRTEIEIKGNITELTASYIVVGGITFSVDNTTQVFDHLNNIIQYSSL
                     QAGKRVEVKGDKTGEATAKASVIKVEGIEDIEIFGKITGINSNSIELNSRVVFVNSNT
                     IYLNRANMPITLNDLAVDQFVEVKMVKNTDNTLTALRIKIEDGTSFTKVNGFSGTITG
                     SSILLPTGSYTINGQTVVIDNNYNVISANQLTTGQQVIVWAVVDPSTNKTALQIQSKI
                     STPTGIDESPVVVNNFVLEQNYPNPFNPSTIISFTIQADQLVTLKVFNALGEEVTTLV
                     NGNLAKGSHSINFNANGLSSGFYLYRLESGNQVQVRKMMLMK"
     gene            1931..2353
                     /locus_tag="VF870_15850"
     CDS             1931..2353
                     /locus_tag="VF870_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253720.1"
                     /translation="MKTQKLIGSITMLLILTLFASCSEDQTTAPPVNTNVPLAKFSDI
                     KAKVFVNCTGAQCHSSSGNQGNLILESSVAYNNLVGVQSVLFPQFKRVEAGNSANSLL
                     IKILKGEVSPRMPLNGNPLSAATIDSIAKWIDLGALNN"
     gene            2384..>2572
                     /locus_tag="VF870_15855"
     CDS             2384..>2572
                     /locus_tag="VF870_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253721.1"
                     /translation="MIARTFFSSMMVLLFIIQLTVLASPQHENVTINFTNMSPHINQN
                     LYLRIVDKSTLKESDRTFV"
BASE COUNT          900 a          413 c          488 g          771 t
ORIGIN      
        1 ttgttcaagg ttatactatt tatgttgatg aatatacaga actccgcggg ccaaataaca
       61 caaccgttat cttttctttc ttccagttaa atgacctggt acaagtccag gctgatagca
      121 ggggcgatgg aacttatctt gcagctagag taaaatccga tgacggtgcc ggaggtggaa
      181 acgaaaatga agtggaatta atcggttatg taacagaagt tggtacctca tctttcgcaa
      241 ttaacggtac ggcatttttt gttgatgtaa atactgaata tcggggcaga catggaaata
      301 ctttttcctt tgaacaaatt gttcccggaa ttctgctcga agtaaaagcg actttacaaa
      361 ctaatggaga tttactagct actcgtgtta aaaccgaaga tgatgaaaat cagcatgaaa
      421 gcgaacttga aataaccggt ttaattgaaa gtcttggagc aaacagttta accgttggac
      481 aacaggagtt ttttgcagat gctcagacca ttgttcttaa tcataataat atgcctataa
      541 atttttcaga tcttaatatt ggtgataaag tcgaagttaa agcatataaa caatctgatg
      601 gttcttactt agctgttaga attaagttag aagatttacc gggaaatgaa attgaattta
      661 ctgcacagat tgaaagtata gttggcccgg atgtaacgat taacggtatt gtattttcca
      721 ctgattcaag cactgttttt cttgatgata acagaatgcc tgttacaatt gcttctttgg
      781 cagttggaat gtgggttgaa gtaaaaggtt taaaaagaca ggacggctct tattatgcaa
      841 gcagaattca gattgaagat tttgttagaa ccgaaataga aatcaaaggc aacattactg
      901 aactaacggc ctcttacata gttgttggcg gaataacttt ttcagtggat aacaccaccc
      961 aggtttttga tcacttaaat aatataattc aatattcttc actacaagcc ggcaagcggg
     1021 ttgaggtgaa gggagataag actggcgaag caacagccaa agcatcagta attaaagttg
     1081 aaggtattga agacattgaa atatttggaa aaattactgg tataaattct aatagcattg
     1141 agttaaatag tcgtgttgta tttgttaatt caaataccat ttatttaaat cgtgccaata
     1201 tgccaattac tctgaacgat ttagccgttg atcaatttgt tgaagtaaaa atggtaaaaa
     1261 atactgacaa tactcttacc gcattaagaa tcaagattga agatggaacg agctttacta
     1321 aagttaatgg ttttagcgga acaataacag gttcatcaat tcttcttcct acaggcagct
     1381 acacaattaa cggccaaacc gtggtaattg ataacaacta taatgttata tctgcaaacc
     1441 aattaactac gggacaacaa gtaatagttt gggctgttgt tgatccatca acaaacaaaa
     1501 ctgctttaca gatccaatca aaaattagta caccaaccgg aatagatgaa tcacctgttg
     1561 ttgtaaataa ctttgtttta gaacaaaatt atccaaatcc atttaatccc tctacgataa
     1621 tctccttcac aatacaggca gatcaattag ttacattaaa agtatttaat gctcttggtg
     1681 aagaagtaac aactcttgta aatggaaatc ttgctaaagg atcacacagc ataaatttta
     1741 atgcaaatgg tttgagttca ggattttatc tatacagatt agaaagcgga aatcaggtac
     1801 aggttaggaa gatgatgttg atgaaataat tgctgagttg taaaagttaa atattcaaaa
     1861 aggcgattat aattaatcgc ctttagtata ggaatgaaca aaactattta taaaaaaaag
     1921 gaaattaata atgaaaacac aaaaacttat cgggtctatt acgatgttgt tgatattgac
     1981 cttgtttgcg tcctgttcag aggatcaaac taccgcacca cctgtgaata ctaatgtgcc
     2041 acttgcaaag tttagtgata ttaaagcaaa agtgtttgtt aattgtacag gagcacaatg
     2101 tcattcgtca tcaggaaatc agggtaatct tattcttgaa tctagtgttg cgtacaataa
     2161 tctggttggt gtacagagtg tgctctttcc tcaattcaaa agagttgaag ccggtaacag
     2221 tgcaaacagc ttactgataa aaatacttaa gggagaagta tctccaagaa tgccgttgaa
     2281 cggaaatcct ttatcagcag caacaataga cagtatagca aagtggatag atctcggagc
     2341 attaaataac taattaacaa aaataaagta aaaaggagcc gctatgattg caagaacatt
     2401 cttttcatcg atgatggtac tgttatttat catacaatta actgtgcttg cttcacccca
     2461 gcacgaaaat gttacaatta actttaccaa tatgtccccg catattaatc agaatcttta
     2521 cttacggatt gttgacaaaa gcaccttaaa agaaagtgac agaacttttg ta
//
LOCUS       DASWBY010000879         9214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_332493, whole genome shotgun sequence.
ACCESSION   DASWBY010000879 DASWBY010000000
VERSION     DASWBY010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332493"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VF870_15860"
     CDS             <1..312
                     /locus_tag="VF870_15860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9253722.1"
                     /translation="MVIGSFIFLSVGITLQGLTNITTENIFYLLWLAVVNTALAFTIW
                     NYTLRTLTAMESSIINGTMLIQIALLAWIFLGEDISFKEIIGMLIASSGAVLVQLKFK
                     K"
     gene            complement(428..1327)
                     /locus_tag="VF870_15865"
     CDS             complement(428..1327)
                     /locus_tag="VF870_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX9253723.1"
                     /translation="MKKSFSLLILIVLLAFACKQTPTADPNYVKEINEWDARRVNRLK
                     ADDGWLNLVGRFWLKQGESTFGSSKDNVIVIESSKLPEHIGSFLFNDTTVTFKAKDDV
                     EVLLEGKPVKEIVLIDDQKKDMTVLQIGSIKFNLIVRDTLYGIRFRDLNSDLVKNFKG
                     VERFPIDESWKLNATFETYNPPKEIEVPNVLGQISKEKSPGAVVFERDGKKYKIDAVD
                     EGGDRLFLIIADETSGEETYGGGRFMYVNKPDSTGTILLDFNKAYNPPCVFTKYATCP
                     MPPLQNYLKLRIEAGEKVYGEGH"
     gene            1420..2883
                     /locus_tag="VF870_15870"
     CDS             1420..2883
                     /locus_tag="VF870_15870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX9253724.1"
                     /translation="MKKNPLEILKEFFGYDSFRGKQEEIIKRITEEQKHSLVLMPTGS
                     GKSLCYQVPALYFDNSTIVISPLIALMQDQVDVLRKKNIPAAFINSTLSKQEREKRLE
                     QFASGKIKLLYVTPERFRKPDFIEQIKKVKIDLLAVDEAHCISEWGHDFRPDYSRIGE
                     FRELLRNPLTIALTATATHDVQEDIIKKLHINKDDIKLFHQGIERPNLRLEAVDVFDD
                     KEKLREIISTLEKFEGCGIIYFSLIKTLERFSEILGDKGFKHGVYHGKLEDRQRKSMQ
                     RDFLSGKQKLILATNAFGMGIDKSDIRFVIHAEVPSSIESYYQEIGRAGRDGKDSLCL
                     LLYNQEDLYTQMEFIKWSNPDANFYSALYDILKRETGLINSSGVDYIREQISFKNKND
                     FRVETALAMLDRYGVTEGQIEKQNLQIVTELPDILQDEDHLKEKLLNDNKKLLSVVNY
                     FRGEKCRRVFISDYFGFPGEKPCGNCDNCTINGITKI"
     gene            2901..4436
                     /locus_tag="VF870_15875"
     CDS             2901..4436
                     /locus_tag="VF870_15875"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9253725.1"
                     /translation="MKSENQKIKTLLLSISLINLSVCLITQAFFSYGIFRDELYYIAS
                     TNHLDFGFVDHPPLSIFILAAWKLLFGDSVFVIRIVPAIISSLTIFILGLFTNKLGGN
                     KTAVIISTTTFMFSAIFLGMNTIYSMNTFDFLFWILSAYIYLRLIQTENKKIWLLLGI
                     ILGFGLLNKTSLLWLGAGIFIGTIFTPLRKDLKTKYPYLAALIALVIFSPYIIWNITH
                     DFAHLEFMRNAATRKYGGLTPFTFIVDQILILNPLTVLIWLSGIIFFFFRNESKNVRA
                     LGFIWLTTFFILLINGHSKGEYMAASYQLLFAGGAVLIEKWSSFNKRSWIKYVITVPV
                     IATEILLAPFARPLLSPENFLKYQSAMGLKPPSNEGHELVGLPQFYSDMFGWEDLAKN
                     VSSVYSTLSDEQKHNTFVYCSNYGKAGAIEYYSKKYPLPKVICPHNSYWYWWPDIKSD
                     ATIIIIGGEIKDHLQALNEVKEVGFHHTEYAMPYENNLKIFIGRGLKISLQEIRQTDK
                     AFI"
     gene            complement(4495..5151)
                     /gene="msrA"
                     /locus_tag="VF870_15880"
     CDS             complement(4495..5151)
                     /gene="msrA"
                     /locus_tag="VF870_15880"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9253726.1"
                     /translation="MKKILKTTYTLFLITFIIIGCNESKSQANKEMKNLMNKNNPNLE
                     IATFGSGCFWCTEAIFERVNGVTSVVSGYSGGTVANPTYKEVCDGTTGHAECTQITFD
                     PSVVSYDELLEIFWKTHDPTTLNRQGNDVGTQYRSVIFYHNDEQKQKAEYYKNKLTEE
                     KIWDKPIVTEITKFEKFYPAEDYHQEYYDNNPNQGYCAYVITPKVEKFEKIFKDKLKK
                     "
     gene            complement(5280..5921)
                     /locus_tag="VF870_15885"
     CDS             complement(5280..5921)
                     /locus_tag="VF870_15885"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9253727.1"
                     /translation="MKVGILGSGVVGQTLATGFIKYGHQVKIGTSNAGKLNNWLSNAG
                     PNGSAASFADAASFGELIVLAVKGTAALNVLEKAGKENLKDKTVIDATNPIADAAPVN
                     GVLKFFTDLNSSLMEELQNKYLQANFVKAFSCVGNAMMVNQDFGGTKPTMFIAGNNDD
                     SKNVVKEILDTFGWEVEDMGKAEAARAIEPLCILWCIPGFLQNRWTHAFKLLK"
     gene            complement(6165..7418)
                     /locus_tag="VF870_15890"
     CDS             complement(6165..7418)
                     /locus_tag="VF870_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253728.1"
                     /translation="MKRLLIINNVLLFFFLLSTSSLFAQSDYEMVQSFKERYQQISDG
                     IKTAANLEDLNNLSAEIDNLKRDFSAKRVILDQSLYPENFNSTFENLNTALELRKEDF
                     TSITVLQTEVTTLKSEVDLLNRRNNELINQISVLETQRRKDAATIEKLEKLVSELRTT
                     LLKRDQLIFSIVDSLTPKLAGDVSSMTQQDKEQVYSQVERNNLLVSVKRSLRDNSRFL
                     EMTSLKAADLESVKEQEQNFVTMWRKIGPKLVDVYANKKDKSAELKEIDNLFNVWANR
                     INKEAWESINEEFSLNNINLQNFNNGKEFVNVITQYISDEIKNYGVKDKSESERAYTV
                     FTDSVWFKNISNEWMPYLLENKLLSVEQKDTIEKKLAEWKSIVSPQDYTWLYAVAGLG
                     LLFVIVLIFILKKKKTPTDTASLKS"
     gene            complement(7415..7873)
                     /locus_tag="VF870_15895"
     CDS             complement(7415..7873)
                     /locus_tag="VF870_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253729.1"
                     /translation="MKHKKIFLVFTSIILLTACTSLLLEPAQFAWPLESVLKVDKEGF
                     VKEERFALNFNTKALFFEETQDSLAYAGKTIRLIRNNEGYYFMTAPNFKNVYVFSVDK
                     SAFSLKTKILISETTGLLNPAFNQRSPYIELLDDGKSIKLTSEGIQEGVK"
     gene            complement(8006..8215)
                     /locus_tag="VF870_15900"
     CDS             complement(8006..8215)
                     /locus_tag="VF870_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253730.1"
                     /translation="MSEKKKFLLRIDEKIYAALEKWAADDLRSINAQIEFLLTDVLKK
                     SGRILQNSSAPQNDNPETDTENPGN"
     gene            complement(8250..8759)
                     /locus_tag="VF870_15905"
     CDS             complement(8250..8759)
                     /locus_tag="VF870_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253731.1"
                     /translation="MKPLSSYIGEDLVLIQPSFFKREYEFRSSSELLAKMYFPSFFSL
                     TAVVEGFDDKYEIIRPSFWKKDIAIRKFAYDMSFASLTTNFFRTKGSIDLQNGKKLNL
                     KFGAFRKSCQVFSETDELLLLFQNKLSFKEKNIVTIQKSSSLIDENPWVVMMIWYLIL
                     QNRKNGAHG"
     gene            complement(8830..>9214)
                     /locus_tag="VF870_15910"
     CDS             complement(8830..>9214)
                     /locus_tag="VF870_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9253732.1"
                     /translation="YDTNEGEKESLRSSPTEVAENLQKELQKRLEVAGVTIVEARITH
                     LAYAQEIAQAMLRRQQAQAIVAARFKIVEGAVGMVQQALQDLSDKKIVNLDEERKAQM
                     VNNLMVALVADHEAMPVINTGTIYQ"
BASE COUNT         2888 a         1623 c         1440 g         3263 t
ORIGIN      
        1 atggtcattg gttcttttat ttttttaagt gtgggaatta ctctgcaagg attaaccaac
       61 attacaactg aaaatatttt ttatcttcta tggcttgctg ttgtaaatac tgctttggct
      121 tttacaatct ggaactacac acttcgtaca ttaactgcga tggaatccag cataattaac
      181 ggaacaatgc ttatacaaat tgctttgctt gcatggatat ttcttggaga agacatttcg
      241 ttcaaagaaa tcatcggaat gttgattgct tcttcgggtg cggttttagt acaacttaag
      301 tttaaaaaat gatttttcct ttgcgatttt tgctaaaaac ctttgcggac tttgcgtgaa
      361 atgcgcttaa ggaatttctc gcaaaggacg cagagtaaaa catgcgaagt tctctaagaa
      421 ttatttctta gtgcccttcc ccgtaaactt tttcaccagc ctcaattctt aacttaagat
      481 aattctgtaa tggaggcatt gggcaggttg catactttgt aaacacacaa ggcggattgt
      541 aagctttatt aaaatctaaa agtattgttc ccgttgagtc tggtttgttt acatacataa
      601 atctgccgcc gccgtaagtt tcttctccac ttgtttcgtc tgcgataatt agaaacaacc
      661 tgtcgccgcc ttcatcaaca gcatctattt tatatttttt gccatcacgt tcaaacacaa
      721 cggcgccagg ggatttttct ttgcttattt gtccaagaac atttggcact tcaatttctt
      781 taggaggatt atatgtttca aaggttgcat taagtttcca gctctcatcg attggaaatc
      841 tttctactcc cttaaagttt tttacaagat cggaattaag atcacggaaa cgtatgccgt
      901 aaagcgtatc tctaacgata agattaaatt taatagaacc aatttgtaaa acggtcatgt
      961 cttttttctg atcatcaatc aaaacaattt ctttaaccgg ttttccttcc agcaaaactt
     1021 caacatcgtc tttagcttta aatgttaccg ttgtgtcatt aaataaaaac gaaccgatgt
     1081 gttcgggcag ttttgaggat tcgattacaa taacattatc tttagaggaa ccaaaagtac
     1141 tttctccttg ctttaaccag aatcttccta caagattaag ccagccgtca tccgccttta
     1201 acctgttaac ccgccttgca tcccattcat taatttcttt aacatagttt gggtctgcag
     1261 ttggtgtttg cttgcaggca aatgcaagta aaacaattaa tatcaaaagt gaaaatgatt
     1321 ttttcatttt aagtcctgta ctttcagtaa atttcttgtt gttaagctac aaattaatta
     1381 ctaattgaaa aatacaattt tcatttacat agaggccgat tgaaaaaaaa tccattagaa
     1441 atattaaaag aatttttcgg gtatgattcc ttccgcggca agcaggaaga aataataaaa
     1501 agaattactg aagaacaaaa acattcactt gttcttatgc caacaggcag cggtaagtct
     1561 ttgtgttacc aggtgcctgc tctatatttt gataacagca caatagtgat tagtcccctt
     1621 attgcattaa tgcaggatca ggttgatgta ctgcgaaaga aaaatattcc agccgctttt
     1681 atcaattcaa ctttatccaa acaggaaaga gaaaaaagac ttgagcagtt tgcgtctggt
     1741 aaaattaaat tgctttatgt gacaccggaa agattccgca aaccggattt cattgaacag
     1801 attaaaaaag ttaaaataga cttacttgct gttgatgaag cacactgtat ttcggaatgg
     1861 ggacatgatt ttcgtcctga ttattcacgc attggtgaat tcagagaatt attaagaaac
     1921 cctctaacaa ttgcactcac tgcaaccgct acccacgatg ttcaggaaga cataataaag
     1981 aaactccaca ttaataaaga tgatattaaa ctttttcatc agggaattga acgcccaaat
     2041 cttagattag aagctgttga tgtttttgat gataaagaaa aacttcgtga aataatttca
     2101 actctggaaa aatttgaggg atgtggaata atttattttt ctttaatcaa aaccttagaa
     2161 agattttctg agattcttgg cgacaaagga ttcaaacatg gagtttatca cggtaagcta
     2221 gaagacaggc aaagaaaatc aatgcagaga gattttctaa gcggaaaaca aaaacttatt
     2281 cttgcaacaa atgcctttgg aatgggaata gataaatctg atattagatt tgttattcat
     2341 gcagaagttc cttcgtcaat cgaatcatat tatcaggaaa ttggtagagc cggaagagat
     2401 ggaaaagatt cgctttgtct tttgctctac aatcaggaag atctttacac acagatggaa
     2461 tttattaaat ggtccaatcc tgatgcaaat ttttattccg ctttatatga tattttaaaa
     2521 cgtgaaacag gcttgattaa ttccagcgga gttgattaca tccgtgaaca aataagcttt
     2581 aaaaataaaa atgattttag agttgagaca gcacttgcta tgctggatcg atatggcgtt
     2641 acagaaggcc aaattgaaaa gcaaaacctg caaattgtga ctgaactgcc cgatattcta
     2701 caggatgaag atcacttaaa agaaaaactt ttaaatgata ataaaaaact tctttctgtt
     2761 gttaactatt ttagaggtga gaaatgccgg agggttttta tttcagatta ttttggtttt
     2821 cccggtgaaa agccctgcgg taattgtgat aactgtacga ttaatggaat tacaaaaatt
     2881 taatttcaaa aataaattgt atgaaatctg agaatcaaaa aataaaaact cttttgctgt
     2941 cgatatctct tattaatctt tctgtctgct taattaccca ggcattcttt tcttacggta
     3001 ttttccggga tgaactttat tacatagcat caacaaacca tttagacttt ggctttgttg
     3061 atcatcctcc attatcgatc tttattcttg ctgcttggaa actactattc ggtgattctg
     3121 tttttgtaat aagaattgtt cccgcaataa tctcgtccct tacaattttt atacttggtt
     3181 tattcacaaa caaactaggt ggtaataaga cggctgttat catttcaaca actaccttta
     3241 tgttttctgc tattttttta ggaatgaaca cgatttattc aatgaatact tttgattttc
     3301 ttttctggat tttatctgct tatatctact tgcgtttaat ccaaacagaa aataaaaaaa
     3361 tatggttact acttggaata attcttggtt ttggattgtt aaacaaaacc agtttgttat
     3421 ggcttggtgc cggaattttt atcggaacaa tttttacccc cttgagaaaa gatttaaaaa
     3481 caaaatatcc ttatttggca gctctaattg cgctggttat tttttctcca tatataatct
     3541 ggaacatcac acacgatttt gctcatcttg agtttatgag aaatgcagca acaagaaaat
     3601 atggaggtct gactcctttt acatttatag ttgatcaaat attaattctt aatccgctaa
     3661 ctgttctaat atggctctcc ggaataatat ttttcttctt tcggaatgaa tcaaaaaatg
     3721 ttcgtgcact tggatttatc tggttaacta cattttttat tttattaatc aacggacata
     3781 gtaaagggga atatatggct gcgtcatatc aattactttt tgcgggcggc gcagttttga
     3841 tagaaaagtg gagttcgttt aataaaagat cctggatcaa atatgtaatt actgttcctg
     3901 taattgccac tgaaatattg ctggctcctt ttgcaaggcc tttgctctca ccggaaaatt
     3961 ttttaaagta tcaatctgct atgggattaa agccgccaag taacgaagga catgagcttg
     4021 ttggtttacc ccagttttat tcagatatgt ttggatggga agaccttgca aaaaacgttt
     4081 cttcggttta ctctacttta tctgatgaac aaaaacataa tacttttgtc tattgcagta
     4141 actacggtaa agccggtgcg attgaatact atagtaaaaa atatccgtta ccaaaggtaa
     4201 tttgtccgca taacagttac tggtattggt ggcccgatat aaaaagcgat gcaactatta
     4261 ttataattgg aggagaaatt aaagatcatc tgcaagcttt aaatgaagtt aaagaagttg
     4321 gatttcacca tacagaatat gcaatgcctt acgaaaacaa tcttaaaata tttatcggcc
     4381 gcggattaaa aatatctcta caagaaataa gacaaactga taaagcattt atataaatag
     4441 aaaatattaa tgatgctggt aatccaaaaa caagataatg ttacctgact ttaattactt
     4501 cttcaactta tccttaaaaa tcttttcaaa cttttctact ttaggagtaa taacgtaagc
     4561 acaataacct tgatttggat tgttatcgta atattcctga tgataatctt ctgctggata
     4621 aaatttttca aattttgtta tctcagttac tattggttta tcccaaattt tttcttctgt
     4681 tagcttattc ttataatatt ctgctttttg tttctgttca tcattgtgat aaaaaattac
     4741 ggatctgtac tgtgtaccaa catcattgcc ctgcctgttt aaagtggttg gatcgtgtgt
     4801 cttccagaat atttccagca gctcatcata gctcacaact gaaggatcaa aagttatttg
     4861 tgtacattcc gcgtgtccgg tagttccatc acagacttct ttataagttg gatttgctac
     4921 agtcccacca gaataaccag atacaacaga ggtaactccg tttactcttt caaaaattgc
     4981 ctcagtacac caaaagcatc ctgatccaaa tgttgctatt tctaaatttg ggttattctt
     5041 attcataaga tttttcattt ctttatttgc ttgtgatttg ctttcattac aacctattat
     5101 aatgaaggtt ataaggaata aggtatatgt tgtttttaat atctttttca tttgttttct
     5161 catcgattct ctgtgcttct ctaatttctt cttagtgatc ctctatgaaa tagttttaaa
     5221 tactatgtac aaagatccgc ggagaattat atagataaat acagattaat ttatattttc
     5281 tactttaaca acttaaatgc atgtgtccat ctgttttgta aaaatccagg tatacaccac
     5341 aaaatgcata gcggttcaat tgcacgtgct gcctctgctt ttcccatatc ttccacttcc
     5401 cagccaaaag tatccagaat ttcttttact acatttttag aatcatcatt attgccggca
     5461 ataaacattg ttggttttgt tccaccaaaa tcctgattaa ccatcattgc gtttccaaca
     5521 cagctaaatg ctttaacaaa attggcttgt aaatatttgt tctgaagttc ttccatcaaa
     5581 gatgaattca aatcagtaaa aaacttaaga actccattta caggtgccgc gtccgcaata
     5641 ggatttgttg catcaataac tgttttatct tttaagtttt ccttaccggc tttttctaaa
     5701 acattaagtg cggcagttcc ttttactgca agaacgatta gctcgccaaa acttgctgca
     5761 tctgcaaaag aagcagccga cccattggga ccagcattac taagccaatt atttaatttg
     5821 ccggcattgc tcgtgccaat ttttacctga tgaccgtatt taataaatcc ggttgcaaga
     5881 gtttgtccaa ctacaccaga acctaatatt ccaactttca tattccctcc gaaaaatttt
     5941 aattattaaa aaatggtttt tacttatact tcgatataaa cataaaacaa taaattttta
     6001 atataaattc catttgtcct tcttacctga ctttatgata gttatcacat ttgaacaatt
     6061 aattgcttgc ttacggagag atgggtgcgc tgctatttag aatacaaaaa agccctgaaa
     6121 aaaaatcagg gctttctaaa atccaattat gaataattta atctttaact ttttaatgaa
     6181 gctgtatctg ttggcgtttt tttcttttta agtataaata ttaatacaat tacaaaaagt
     6241 aaacctaatc cggcaacagc ataaagccat gtataatcct gcggtgaaac tattgatttc
     6301 cattctgcta gtttcttttc tatggtatct ttctgttcaa ctgaaagcaa tttattctcc
     6361 agtaaatatg gcatccattc gtttgaaata tttttgaacc aaacgctatc agtaaaaaca
     6421 gtgtacgctc tttcagattc acttttatct ttaactccgt agttttttat ttcatctgaa
     6481 atgtattggg taataacatt aacaaattct tttccgttgt taaaattctg aagattgata
     6541 ttgtttaaag agaactcttc atttatggat tcccatgctt ctttatttat tctattagcc
     6601 caaacattaa acaaattatc aatttctttt aattccgcag atttatcttt tttatttgca
     6661 tacacgtcta caagcttcgg tccaatcttg cgccacatag taacaaagtt ttgttcctgt
     6721 tcttttacag attcgagatc agcagcttta agggatgtca tttcaagaaa tcggctgtta
     6781 tcccgcaaag atctttttac tgaaacaagt agattatttc tttcaacctg agagtacact
     6841 tgttccttat cttgctgagt catagacgaa acatcacctg caagtttggg tgttaagcta
     6901 tctacaatac taaaaattaa ttggtctctt ttaagaagag ttgttcttaa ttcagaaaca
     6961 agtttttcta atttttctat tgtagcggca tcttttcttc gttgtgtttc cagaactgaa
     7021 atttgattta taagttcatt gtttcttctg tttaaaagat caacttcaga ttttaatgtt
     7081 gtaacttctg tttgtaaaac ggttatcgaa gtaaaatctt cttttcttaa ttcaagcgca
     7141 gtgttaagat tttcaaaagt agaattaaaa ttttcgggat agagactttg atccaggata
     7201 actcttttgg cagaaaaatc tctttttaaa ttatcaattt ctgcggataa gttatttaga
     7261 tcttccaaat ttgctgctgt tttaatacca tcagaaattt gctgatatct ttccttaaaa
     7321 ctctgcacca tttcataatc tgattgagca aataatgatg aggttgataa tagaaagaaa
     7381 aaaagtaata cattattaat aattaataat cttttcattt tacaccttcc tgaattccct
     7441 cagaagtaag ttttatactt ttcccatcat ctaaaagttc aatatatggg cttcgttgat
     7501 taaaggcagg atttaataat ccggttgttt cgctgattaa gatttttgtc ttaagactaa
     7561 atgcactttt atcgactgaa aaaacataaa catttttaaa gttaggagct gtcataaaat
     7621 aataaccttc attatttcta ataaggcgta tagtttttcc agcataggct aatgaatcct
     7681 gtgtttcttc aaagaaaagc gctttagtat taaaatttaa agcaaacctt tcttctttaa
     7741 caaaaccttc cttatcaacc ttaagaacgg actcaagcgg ccaggcaaat tgtgccggct
     7801 ctaacagcaa agaagtacat gccgtgagta atataattga agtaaatact aaaaatattt
     7861 ttttgtgttt cattttctct ccattgtaaa atcgtgagtg taaacttaag aattccaaat
     7921 acatcgcgaa agaccaaaat tctaaaatta tattctaaaa cttgaatttt tgaggttttt
     7981 tagtgggata aagcttgaaa taacattaat tacccggatt ttcagtatca gtttctggat
     8041 tatcgttttg gggtgctgaa ctattctgaa gaattcttcc tgattttttt aatacatcag
     8101 ttaacaaaaa ttctatctgc gcatttatac tgcgcaaatc atcagccgcc catttttcca
     8161 gagcagcata aattttttca tctattctta atagaaattt tttcttctca ctcatattat
     8221 caccaataca catttataaa atataatcat caaccgtggg ccccgttttt tctgttttga
     8281 agtatcaaat accaaatcat cattacaacc catggatttt catcaattaa agaagaagat
     8341 ttttgaattg ttacaatatt tttttcttta aaagataatt tgttttgaaa caataatagc
     8401 agttcatccg tttccgaaaa aacctggcag gattttctga atgcaccaaa tttcagatta
     8461 agtttttttc cattctgtaa atcaatcgat ccttttgttc taaaaaagtt agttgttaaa
     8521 gatgcaaagg acatatcata agcaaacttt cgaatggcaa tatctttttt ccagaagcta
     8581 ggtcttataa tctcgtattt atcatcaaat ccttctacaa cggctgtcag actaaagaag
     8641 cttggaaagt acatctttgc cagaagttcg cttgaagatc tgaactcata ctcacgttta
     8701 aagaatgacg gttgaataag tactaaatct tcaccaatat aacttgaaag tggtttcatt
     8761 ttttttactg atttacgtta aatagagatg acatctttca aaaagatgtc atctcttaat
     8821 attgatttat tattggtaaa tagttcccgt attaataaca ggcattgctt catgatctgc
     8881 aaccaatgct accatcaggt tatttaccat ctgtgctttt cgttcctcat caagattaac
     8941 aattttttta tcactcaaat cctgcaatgc ctgctgtacc attcccacag cgccttcaac
     9001 aattttaaat cttgcagcaa caattgcctg tgcctgctgt ctgcgaagca ttgcttgcgc
     9061 aatttcctgt gcgtatgcaa ggtgagtaat tcttgcttca acaattgtaa ctcctgcaac
     9121 ttccaatctt ttttgaagtt ctttctgaag attctcggca acttctgttg gactgcttct
     9181 taaagattct ttttctcctt cgtttgtgtc gtaa
//
LOCUS       DASWBY010000880         2633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_332515, whole genome shotgun sequence.
ACCESSION   DASWBY010000880 DASWBY010000000
VERSION     DASWBY010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332515"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VF870_15915"
     CDS             <1..381
                     /locus_tag="VF870_15915"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX9253733.1"
                     /translation="IIVSLIGTEFDIDYSNKIIFIEEVGEEPYRIDRMLTQMIQAGKF
                     ENAAGVMMGIFRKCEVKKESDLTAKSFTLMEVLQDRFSGLKIPVIYGMSFGHVKDKFT
                     IPFGALAELDADKSTFALLEKAVN"
     gene            476..1018
                     /locus_tag="VF870_15920"
     CDS             476..1018
                     /locus_tag="VF870_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253734.1"
                     /translation="MKKTLIILSLIIIIQLTGCIVFHSVSYEVNVNDDGTGTAMLTIE
                     DINTDAATKEMIDEDVKSILEYGLKSQEFVDDQKKDGKMITSRNLMVEREKLNALVRY
                     DFNDLSEVEGLQFDDPYYYLTIPAEDSIISTNGQITKTSEYQRIVWDKSIKTLKFKMY
                     SDDTSREGLTSLAPYYLKEN"
     gene            1018..>2633
                     /gene="priA"
                     /locus_tag="VF870_15925"
     CDS             1018..>2633
                     /gene="priA"
                     /locus_tag="VF870_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX9253735.1"
                     /translation="MFVEVVFPLPFRKAFTYSVPKDLQEFTKVGVRAVAPFGKRTLSG
                     FIINIPASISLNKDEVKPITDILDDKPIFTNKTLKFYEWLSEYYLCSLGEALKLLVPQ
                     GTDVETKRKIIVDKDFITELLSKEKKKDSIKYKILLELSKRDELSFSTLQKSVKKKNI
                     YSQIRLLHNQGAVTIVDEVEGVKVKAKKVKFVKLAKEIAEIYASFPELDRKSPKQVKI
                     LLKMIEAKGIALPVAELLHKTESSQSSLSGLEQKGFVTVFEKEVDRRYKEQYEEDHQK
                     LMLTKQQQEVVDNVLDVLNKNAFHTFLLHGVTGSGKTQVYIELAKKTLAKNKTVLILV
                     PEISLTPQITSRFFNNFGDTVTVVHSRMSAGERFDSWRRVLKGKSKIVIGARSALFAP
                     LSNIGLIVVDEEHDASYKSADMIPKYNARDAAVVLGNIFSCPVLLGSATPSIESMHNA
                     ETGKYKLLSLLTRIDDAKLPKITFVNISHEKNKTKSETVFSKILIDKIDDRLKKNEGV
                     IILQNRRGFSTQIFCNDCGEVEMCENCSVP"
BASE COUNT          954 a          376 c          506 g          797 t
ORIGIN      
        1 attatcgttt cactaattgg gacggagttt gatattgatt actccaataa aataattttt
       61 attgaagagg ttggcgaaga accttatcga attgatagaa tgttaacgca gatgattcaa
      121 gccggaaaat ttgaaaatgc agcaggagta atgatgggaa ttttcaggaa gtgtgaggta
      181 aaaaaagaaa gcgacttaac tgcaaaatca tttacattga tggaggtttt acaggatagg
      241 tttagcggtt taaaaattcc tgtcatttat ggaatgtcat ttgggcatgt aaaagataaa
      301 tttacaattc cttttggtgc gctggcagaa cttgatgcag ataaatcaac atttgcatta
      361 ttagaaaaag cagttaatta attttataat aaataactac cctgagcaaa aaggagtatt
      421 gcttcgtaaa ccttgtgatt cttctttggt aacttcatgg taaaggagat ttaaattgaa
      481 aaaaacacta attattttat cactaataat cataatccag cttaccggtt gtatagtgtt
      541 ccattctgtt tcttacgaag ttaatgtaaa tgatgacgga accggaacag caatgctgac
      601 tattgaagat attaatacag atgcagctac aaaggaaatg attgatgaag atgtaaaaag
      661 tattcttgaa tatgggttaa aaagtcaaga gtttgttgat gatcagaaaa aagacggtaa
      721 gatgattaca tcacgaaatc ttatggttga aagagaaaaa ctaaatgctc tggttcgtta
      781 tgattttaat gatttaagtg aagttgaagg attgcaattt gatgatccat attattattt
      841 aaccattccg gcagaagaca gtataatttc tacaaatggg cagatcacaa aaacctctga
      901 gtatcagaga attgtttggg ataaatctat taaaacatta aaatttaaaa tgtacagcga
      961 tgatacaagc agagaaggtt tgacatcttt agccccgtac tacttgaaag aaaattaatg
     1021 tttgttgaag ttgtttttcc attgccattc aggaaagcat ttacttactc ggttccaaaa
     1081 gatttgcagg aatttactaa agtaggagtg cgtgctgttg caccatttgg aaaaagaact
     1141 ttatcaggat ttataataaa tattcccgct tcaatttcat taaataaaga tgaagtaaag
     1201 cccattacag atatacttga tgacaaacca atcttcacaa acaaaacatt aaagttttac
     1261 gaatggcttt ctgaatatta tttatgctca cttggtgagg cattaaaatt acttgtaccg
     1321 caggggaccg atgttgaaac gaaaagaaaa attatagtag ataaagattt tataacagaa
     1381 cttctttcaa aagaaaagaa aaaagattca ataaagtata agatactttt agagctttct
     1441 aaaagagacg aattaagttt ttcgacactt caaaaatcag taaagaaaaa aaatatttac
     1501 tcacaaattc gtttacttca caatcagggg gcggttacta ttgttgatga agttgaaggc
     1561 gtaaaagtaa aagcaaagaa agtaaagttt gtaaaacttg caaaagaaat cgctgaaatt
     1621 tatgctagct ttcctgaact ggacagaaaa tcaccgaagc aggtaaagat tttacttaag
     1681 atgattgaag caaagggtat tgcacttccg gttgctgagt tgcttcataa aacggaatct
     1741 tctcaatcat cattaagcgg acttgagcag aaaggttttg taacggtatt tgaaaaagaa
     1801 gttgatcgca gatataaaga gcaatacgaa gaagatcatc agaaactgat gctgacaaaa
     1861 caacagcaag aggttgtaga taatgtttta gatgtcctaa ataaaaacgc atttcatact
     1921 tttcttttgc acggagtaac tggaagcggt aaaactcagg tttacattga gcttgcaaaa
     1981 aaaactctcg caaaaaataa aactgtttta attcttgttc ctgagatttc tttaaccccg
     2041 cagattacat ctcgtttttt taacaacttt ggagatacag taacagttgt tcacagtcga
     2101 atgtctgctg gagaaagatt tgattcctgg aggcgggtgt taaaaggaaa atctaaaatt
     2161 gtgattggtg cacgctcagc attatttgcg ccgctaagca atattggatt gatagttgtt
     2221 gatgaggaac acgatgcgag ttataagagc gcagatatga ttcccaaata caatgcccgg
     2281 gatgctgcag ttgtgcttgg aaatatattt tcttgtcctg ttcttcttgg ctctgcaact
     2341 ccatcgattg aaagtatgca caatgctgaa accgggaaat acaaattact ctcgcttctc
     2401 actagaattg atgatgcaaa acttcccaaa ataacgtttg tgaatatctc acacgaaaaa
     2461 aataaaacta aatcagaaac tgttttttca aaaattctta tagataaaat tgatgatcga
     2521 ttaaagaaaa acgaaggtgt aataattctt caaaacagaa gaggattttc aactcagatt
     2581 ttttgcaatg attgcggtga agttgaaatg tgcgaaaatt gttctgtgcc aat
//
LOCUS       DASWBY010000881         5003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_563400, whole genome shotgun sequence.
ACCESSION   DASWBY010000881 DASWBY010000000
VERSION     DASWBY010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563400"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VF870_15930"
     CDS             complement(<1..1431)
                     /locus_tag="VF870_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253736.1"
                     /translation="MNQKLQNISNIIRQSEKLDKEQKEILLKAIKDVDKELEITAFKL
                     ERTEKVKKTTAILLEETIEELEQKRKAVEEHNRELEIESSLERVRAVAMSMSRSEDLL
                     NICEASFKEFKKLDFNNIRNAIIHIPNDEQKYFMDYDYSEFTGGAITKIEYGSHPIVD
                     EYLEKIRSAEDAYFEVVITKDQLSGWKDFRKKSGQQDDPRLEDSTALYYYLFSIGIGD
                     IGISTFNQIDESQIKILKRFRNVFDLAYRRYNDIALAEAQAREAEIQLALERVRARAI
                     AMHKSDELLEAGELLYREITRLGIVCLTSGYVLIDEKENIGWNYAASPKDGKIMSVPV
                     GIPHLETEVMCTVTTSWKKGEPYSIIQLDPEATIKHQTFIAERSTNFPLTAKELIAIS
                     PEQVVLHNFNFKHGYLLIVGGVLLTAEQIDMMVRFSKVFEMTYRRFLDLQKAEAQARE
                     AQIEAALEKVRSRTMAMHQSYELADSA"
     gene            complement(1440..2579)
                     /locus_tag="VF870_15935"
     CDS             complement(1440..2579)
                     /locus_tag="VF870_15935"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX9253737.1"
                     /translation="MKVKSIKGKSTEEIKNELTKSLADGFKPTLAIVFLSVSQDRNAI
                     CKLLDSEDIAIFGATTSGEFIDEVTEKGTVVMLFLDIKKDNFQILFDEYPEKKYRETA
                     AAIALKAKSKFSNPAFLISGSHMETDAEQLLFGFEDVAGKDVNVFGGMASDDYTFTEQ
                     FVFSNGWETNRGIVALALDEDKIRIKGKATCGWKAVGTEKTVTKSEGNHVYTVDNIPV
                     LDLTSKYGGLENVSPDNAGLLLDIAANLPLQLQREKGDPVMRPGLVVDWDDRSFYCSG
                     SVPQGAKVRFSIPPDFDVMEKVIKGAEDLKSAEMPEADALVVFSCAGRILAFGPMMNM
                     EIEGLKKIWNVPMVGMFSNAELARATGGNLEMHNLTTCCVVLKEK"
     gene            complement(2684..3823)
                     /locus_tag="VF870_15940"
     CDS             complement(2684..3823)
                     /locus_tag="VF870_15940"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX9253738.1"
                     /translation="MKAKSIKGKSAEEIQSALTESIADGFKPTLAIVFLSVKLDRDSI
                     CKIFDKKGITIFGSTAAGEFISGDLEKESAAILLLDMDKNNFLVHIEERSGKSKLRTA
                     AEIGKKGLDRFSNPAYLVATGGSIIDGETIISGINSTSNKNSIVYGGIAGDELRGTET
                     HLFTNNKSTQDGIIALIVDHDKIQLTGLASHGWKPIGTIRTITDCEDCKVYTIDDEPA
                     LDIMTKFLGISLEDYPGDELINNVGNMDPIQLMRDDASTITRAIRNLNKKEKSILLAG
                     PVTRGTKIRFSLPPDNNIIQQVTEECRYLKEIYQPQAEAMIMFSCIGRYYSLGPLVSS
                     EIEEVKSVWNSPMAGLFCYGEIGNSLKGKQEFHNNTCCIIVMKER"
     gene            complement(3916..>5003)
                     /locus_tag="VF870_15945"
     CDS             complement(3916..>5003)
                     /locus_tag="VF870_15945"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX9253739.1"
                     /translation="DSMADGFKPTLAIVFMSIKQNREIICALLNNLEIQIFGSTTSSE
                     FINGDISEGGITIMLLDINPTYFKIVFLETGNSSVYEMSKQLGASGKNSFVRPAFIIA
                     SGWINIDGEEIIKGIEDGFEGVPTIFGGMAGDDISLIGPIVFTNNKNSISGLVVLIID
                     EDRVSVNGIATCGWKPVGVTKTVTKSSGKIVYTIDNQPAFDLVIKYLGLNKEDMSKVT
                     DSIVNFGANYPLQLERDDAPPVMRTAQLGNLADRSIICAGNVQQGTKFRFSVPPDFEV
                     IDTVVNECSDMKNHGAHDADVVIMFSCISRYYSLGIMASEEIQRVMEIWNAPFIGFFT
                     YGEYGKSKKGKHEFHNNTCCVVALKEK"
BASE COUNT         1410 a         1061 c          769 g         1763 t
ORIGIN      
        1 agcagaatcg gcaagttcgt aactctgatg cattgccata gttcttgatc ggactttttc
       61 taatgccgct tcaatttgtg cttcccttgc ctgtgcttca gctttttgca gatcaagaaa
      121 acggcgataa gtcatttcaa aaacttttga aaatcttacc atcatatcaa tttgttctgc
      181 agttagtaaa actccaccaa caattaataa ataaccgtgt ttaaagttga aattatgcag
      241 cactacttgt tcgggtgaaa ttgcaataag ttcttttgca gttagcggaa agtttgtgct
      301 tcgttctgca ataaatgttt gatgttttat agtagcttca ggatcgagct gaattatact
      361 ataaggttct ccttttttcc aacttgtggt tactgtgcac attacttccg tttccaaatg
      421 aggaattcca actggcacag acatgatttt tccatcttta ggactagctg cataattcca
      481 tccaatattt tctttttcat caatcagcac ataaccgctg gtaagacata caattcctag
      541 tctggtaatt tctctgtaca ataactcacc agcttcaagc agttcgtcac ttttatgcat
      601 agctattgca cgtgcacgca ctctttcaag agccagctga atttcagctt ctcttgcctg
      661 tgcttctgca agtgctatat cattgtacct tcggtatgct aaatcaaata catttctaaa
      721 tcttttaaga attttaattt gtgattcgtc aatttgatta aatgtagata tgccgatatc
      781 tccaattcca atagagaaca gatagtaata aagtgctgtt gaatcttcta atcgtggatc
      841 atcctgctga ccggattttt ttctgaagtc tttccatccg ctcagctgat ctttagttat
      901 aacaacttca aaataagcat cttccgcact tctgattttt tccagatatt catcaacaat
      961 gggatgtgaa ccatattcaa ttttcgttat tgcaccacca gtaaattccg aatagtcata
     1021 atccataaaa tatttttgtt catcatttgg aatatgaatt atagcattcc ggatattatt
     1081 aaaatctaac tttttaaatt ctttaaatga tgcttcacaa atatttaata aatcttcaga
     1141 tctactcata ctcatcgcaa cggctctgac tcgttcaaga gaagattcaa tttcaagttc
     1201 acggttgtgt tcttcaactg ctttcctttt ttgttcaagt tcttcaattg tttcttcaag
     1261 cagaattgct gttgtctttt taactttttc agttctttca agtttaaatg cggttatctc
     1321 taattcttta tccacatctt ttattgcttt taagagaatc tctttttgct ctttatccag
     1381 tttttcagat tgccgaataa tgttagatat attttgaagt ttttgattca tttaaatttt
     1441 tatttttctt ttaaaactac acaacatgtc gtcaaattgt gcatttctaa atttcctcca
     1501 gtcgctctgg ccaattctgc attggagaac atccctacca tgggtacatt ccaaattttc
     1561 tttaatcctt ctatttccat attcatcatg ggtccgaaag caagaattct gccggcacaa
     1621 ctaaaaacca ccagtgcatc agcctcaggc atttcagcac ttttgagatc ttcagctcct
     1681 ttaatgacct tttccatcac atcgaaatca ggtggaattg aaaaccgtac cttggctccc
     1741 tgaggtactg aaccgctgca ataaaaagat cgatcgtccc agtcaaccac taggccggga
     1801 cgcatgactg ggtccccttt ttcccgttgt aattgcaaag gaagatttgc ggcaatatcg
     1861 agtaacaatc cggcattatc cggtgaaaca ttttctagtc caccgtattt tgaggtgaga
     1921 tcaagtacag gaatattgtc cactgtgtat acatgatttc cctcactttt agttaccgtt
     1981 ttttcagtgc ccacagcttt ccagccacag gttgcctttc ctttgatcct gattttgtcc
     2041 tcatcgagcg ctagtgcgac gataccccga tttgtttccc acccattgct aaaaacgaat
     2101 tgttcggtga aggtataatc atcagaggcc atgccgccaa aaacattcac atccttgcct
     2161 gctacatctt caaatccaaa cagtaattgt tcggcatcgg tttccatatg ggagccggaa
     2221 atcaggaagg caggatttga aaatttactt tttgctttta aagcaatggc tgctgctgtt
     2281 tccctgtatt ttttttcagg atattcatca aacaagattt ggaaattatc ttttttgata
     2341 tccagaaaca gcatcactac agttcctttt tctgtcacct catctatgaa ctcaccgcta
     2401 gtggtagcgc caaaaatcgc aatgtcttca gaatcaagca acttgcatat cgcattcctg
     2461 tcctgactca ccgacaggaa cacaatggcc agggttggtt taaatccatc agccaagctt
     2521 ttcgtcagtt catttttaat ttcttctgtt gattttcctt taattgattt tactttcata
     2581 atctatttta ctttttatta ttaaatattt acttgaattg tcacactgag ctttacgaag
     2641 tgtgaacaat tcttagtcat ccttcgactt gctcatgatg aaattatctt tctttcatca
     2701 ctattataca gcaagtatta ttatgaaatt cctgcttacc ttttagcgaa tttccgattt
     2761 cgccatagca gaagagacct gccattggag aattccaaac actttttact tcttcaatct
     2821 cagatgacac taatgggccc aaagaataat atcttccaat acaggaaaac attatcattg
     2881 cctcggcttg aggttgatat atttctttca aatacctaca ctcttctgta acctgctgaa
     2941 taatattatt atccggaggt aaagaaaatc tgatttttgt tccgcgcgtt actggaccag
     3001 caagtaatat tgatttttct tttttattaa gatttctgat cgctctggtt attgtacttg
     3061 catcatccct catcaattgt ataggatcca tattccctac attatttatt aattcatctc
     3121 ctggataatc ttctaaagaa ataccaagaa attttgtcat aatatcaagt gctggttcat
     3181 catcgattgt gtaaaccttg caatcttcgc aatctgtaat tgttcttatt gttccaattg
     3241 gtttccaacc atgtgaggct aaaccggtta attgtatttt atcatgatct acaattaatg
     3301 caattattcc atcctgagtg gatttattat ttgtaaataa atgtgtttca gtgccgcgca
     3361 actcgtcacc ggctatacct ccatatacaa tagagttttt attcgaagta ctatttatgc
     3421 cgctaataat tgtttcgcca tcaatgattg accctcctgt tgcaactaga tacgcaggat
     3481 ttgaaaatct atctaaaccc tttttgccga tttctgccgc agttcttaat ttactttttc
     3541 cgcttctttc ttcaatgtgc actaaaaaat tattcttatc catatcaagc aaaagaattg
     3601 cagcagattc tttttctaaa tctccactaa taaattctcc tgcagcagtt gatccaaata
     3661 ttgtaattcc ttttttgtca aatattttgc agatagaatc tcgatccagt tttacagata
     3721 aaaatacaat tgccaatgtt ggtttaaatc catcagcaat actttcagtc aatgccgatt
     3781 gaatttcttc ggctgatttt cctttaattg attttgcttt cataacattt tttgtattta
     3841 tttaatgatc atattatttg ttacaatttg acccgcttcg actcctcagc gaggacagca
     3901 tgttctgtgt gacatttatt tttctttcaa cgctactaca caacacgtgt tgttgtgaaa
     3961 ttcgtgtttc cccttttttg attttccata ttcaccgtaa gtaaagaaac caataaaagg
     4021 ggcgttccaa atttccatta ctcgttgtat ttcttcgctt gccattatgc ctaatgaata
     4081 atagcgactg atgcaggaaa acataattac gacgtcggca tcatgagcgc catgattttt
     4141 catatccgaa cattcattga caactgtatc gatgacctca aaatctgggg gcacagaaaa
     4201 acggaatttg gtgccctgtt gcacattgcc cgcacaaatt attgaacggt ctgcaagatt
     4261 tcccaattga gctgttctca ttacaggagg cgcatcatct ctttcaagtt gtagcgggta
     4321 attggctccg aaattgacta tggaatcagt aactttgctc atgtcttcct tattgagccc
     4381 aagatatttt attaccaggt caaaggccgg ctgattatct attgtgtaaa caatttttcc
     4441 ggaactttta gtaacagttt tggttacacc tacaggcttc cagccgcaag ttgcaatacc
     4501 attaacactc actctatctt cgtcaattat taagacaacc aatcctgaaa tactgttttt
     4561 attatttgta aaaactatag gtccaattaa agaaatatca tccccagcca tgccgccaaa
     4621 aatggttggg actccttcaa aaccatcttc aattccttta ataatctcct ctccatcaat
     4681 atttatccac ccggaagcaa taataaaggc aggccgaaca aaactgtttt taccgctggc
     4741 gcccaattgt tttgacattt cataaactga actgttacct gtctctagaa aaactatttt
     4801 aaaataagta ggatttatat cgagtaacat aatcgtaata ccaccttcgc tgatatcacc
     4861 gttaataaat tcactggatg ttgtagaacc gaaaatctgt atttccagat tgttcaataa
     4921 tgcacaaata atttccctat tctgcttaat agacataaaa acaatagcaa gagttggttt
     4981 aaaaccatca gccatagagt ctc
//
LOCUS       DASWBY010000882         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_298540, whole genome shotgun sequence.
ACCESSION   DASWBY010000882 DASWBY010000000
VERSION     DASWBY010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298540"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VF870_15950"
     CDS             complement(<1..563)
                     /locus_tag="VF870_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX9253740.1"
                     /translation="MKIAVCVSHVPDTATRIKIGADRKAIDPAGVIYIINPYDEFAVE
                     EALKTKEKIGGDSIVYAISVGSDANKESIRKALAMGVDEGVLLKDDNPRDSFGIAHAL
                     ADEIKSLGCEIAFFGKQSVDFDNSITGQVTAELLGYNCVPVVVDFKLDGSKITAEREI
                     EGGREVVETELPAVITTQKGLNEPRYAS"
     gene            725..1015
                     /locus_tag="VF870_15955"
     CDS             725..1015
                     /locus_tag="VF870_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX9253741.1"
                     /translation="MELKPDETRQVEITTEETSDVNLSSRVVLYNDDWHTFEDVIAQL
                     IIATQCSFEKARGFAFEVHVKGKAIVFSGSMSACLKVSSVLEEIALHTQILT"
     gene            complement(1012..1422)
                     /locus_tag="VF870_15960"
     CDS             complement(1012..1422)
                     /locus_tag="VF870_15960"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX9253742.1"
                     /translation="MIANNLIVRDSSVHGLGLFSAVEHKEGQIITIINGDLINADECV
                     RREEEGNVYIFYKNEDEYIDASNHSQLRYLNHSCDYNCDIDEDENGNLILFAVTDIQP
                     GEELTIDYGYEEIYNYCSCNDCENKSEIVSEGNH"
     gene            1890..2435
                     /locus_tag="VF870_15965"
     CDS             1890..2435
                     /locus_tag="VF870_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX9253743.1"
                     /translation="MKNFAYFLTALLFAPFIFIQAQNGNTYVGVETCGMCHKTEKQGS
                     QLSIWQNSAHSKAFQTLKTEKANQIATEKGFKTPAAETPECLKCHVTGYNLDASMLGK
                     KFKVEDGVQCETCHGPGSAYKDMKVMKDKDLAVKNGLVMHDKLENFCIGCHNAESPTY
                     TDMNIEEAWQKIKHTIPKTEN"
     gene            2469..>2744
                     /locus_tag="VF870_15970"
     CDS             2469..>2744
                     /locus_tag="VF870_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253744.1"
                     /translation="MKKFILLLFLGATAFISAQNINGRFSSSLYTFERYDSLNSSEQY
                     LRAYEMLNLNLNYDKVSIRSYLNYETDVKNDLETDPRLRFYNLYLEAR"
BASE COUNT          963 a          460 c          416 g          905 t
ORIGIN      
        1 gaagcatatc ttggttcgtt taatcctttt tgagttgtga ttaccgcagg cagttcagtc
       61 tccacaactt ctcttccgcc ttcaatttca cgctccgcag taatttttga tccgtccaat
      121 ttaaaatcaa cgacgactgg aacgcaatta taaccaagta attcagcagt cacctgtccg
      181 gtgatcgaat tatcaaaatc aacagattgt ttaccaaaaa aagcaatctc acaacccaga
      241 gatttaatct catcagcaag agcgtgggca attccaaaag aatctcttgg gttatcatct
      301 tttaacaata caccttcatc aacacccatc gctaatgctt tgcgtattga ttctttattt
      361 gcatcactcc caacactgat agcataaact atcgaatcgc cgccaatttt ctctttagtt
      421 ttaagtgctt cttcaactgc aaattcatca taaggattta tgatataaat aacgccagct
      481 ggatcaattg cttttctatc cgctccgatt tttattcgag tagcagtgtc tggtacatga
      541 ctaacacata ctgctatttt catttaacct ccgtaaagtt cttagttttt gatacaaaaa
      601 tataaagaca cagtgctcta aaatcaatta agtgaggatt tattatttgc taattaatta
      661 tttaggtatt gtataacttt aattaaaact tttataaaaa aaatcaaatc agtatttttt
      721 agctatggaa ttaaaacctg atgaaaccag gcaagttgag ataactacag aagaaacttc
      781 ggatgtaaat ctttcttcaa gagttgtatt atacaacgat gattggcata catttgaaga
      841 tgttatcgcc cagctaatta tagccacaca atgcagcttt gaaaaagcac gaggttttgc
      901 atttgaagtt cacgtaaaag ggaaagcaat agtattcagc ggttcaatga gtgcctgctt
      961 aaaagtttcc tccgttctgg aggaaatagc tttacacacg caaatcttaa cttagtggtt
     1021 accttcagaa actatttcgg atttattttc acaatcattg caggaacaat aattatatat
     1081 ctcttcgtaa ccataatcaa ttgtcagttc ttcgcctggc tgaatatccg taacagcaaa
     1141 taatattaaa ttaccattct catcttcgtc aatatcgcaa ttgtaatcgc aggaatgatt
     1201 taaatatctt aattgcgaat gattggaggc atcaatgtat tcatcttcgt ttttatagaa
     1261 tatataaaca ttaccttctt cttctcttct cacgcattca tcagcgttaa ttaaatcacc
     1321 gtttattatt gttataatct gaccttcttt atgctcaacc gcagaaaaaa gccccaaacc
     1381 atgcactgat gaatctctga caattaaatt atttgcaatc atatataata ctttatagat
     1441 atttaagttt aattagaaaa tagttttgtt taatcctgat ctgatttata aaatgggaag
     1501 ctgtataaat cacctgaagt tgaccagata taaaaattat aaaagaaaat ttttatactg
     1561 cacaatcata aataaaaaaa atcacttata ttagtccaat tatccttaat agaggacctg
     1621 cttatctaca aaatattttc aatttccgtt taacaattat cagtaatggg agagtatcga
     1681 tataattttc caaaacaaag aattaagatt gtatgagaat aataaaattg gactattttt
     1741 ttggtaccta aattgtttta cagaataggt taaagattta gtgcaattaa aaaaaatcga
     1801 tttttataat aatgaacgtt gtttttagtt gcctttatta ataattcttt gaatattaca
     1861 atagaattaa tcatcattta ggagatctta tgaaaaactt tgcttatttc cttaccgcac
     1921 tattattcgc accattcata tttatccagg ctcaaaacgg taacacttat gttggtgtag
     1981 aaacatgtgg catgtgccat aaaactgaaa aacaaggcag ccagctttca atatggcaga
     2041 acagtgctca ctcaaaagca tttcagactt taaagactga aaaagctaat caaattgcaa
     2101 cagaaaaagg atttaaaaca cctgcagcag aaactccgga atgtctaaaa tgtcatgtta
     2161 ccggttataa cttagatgca tcaatgctag gtaagaaatt taaagttgaa gatggtgttc
     2221 aatgcgaaac atgtcatgga cctggatcag cttacaaaga tatgaaagtg atgaaagata
     2281 aagatttagc tgtaaagaat ggtttagtaa tgcatgataa acttgaaaat ttctgtatag
     2341 gttgtcataa tgctgaaagt ccaacatata ctgatatgaa tatagaagaa gcttggcaga
     2401 agattaaaca cacaatacca aaaacagaaa attaatttta ttagagaata aatcttaaag
     2461 aaaattaaat gaaaaaattt atacttctgc tctttctagg agctactgca tttatttcgg
     2521 ctcaaaatat taatggtaga ttttcaagct ctctttatac ttttgaaaga tatgattctc
     2581 taaattcctc cgaacagtat ttgagagctt atgaaatgtt gaatcttaat cttaattatg
     2641 ataaggtttc tataaggtca tatctgaatt atgaaacaga tgtgaagaat gatctggaaa
     2701 ccgatccaag gttaagattt tataatctgt atttggaagc acgg
//
LOCUS       DASWBY010000883         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_298667, whole genome shotgun sequence.
ACCESSION   DASWBY010000883 DASWBY010000000
VERSION     DASWBY010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298667"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3241
                     /locus_tag="VF870_15975"
     CDS             <1..3241
                     /locus_tag="VF870_15975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9253745.1"
                     /translation="LWVSTGGAGLHINDKKQKQFKLYANQPNNKYSLSSTAVMSVYED
                     RTGTRWIGVRDGGLNRMEKGSNKFIHYKHNPLDPNSLSDNNVSSIYEDKQGILWVGTS
                     NGGLNKFDRKTEKFFNYKHNPSNPKSISDNRILTITEDNSGKLWIGTNFGGLNQFNRD
                     DETFTHYRFDKDDPNSISSDFVSELYIDLSGRLLVATKEGLDEFDQKNKKFLHYNLNE
                     KGSDKIRHTRVTALYQQKNGILWIGTFDRGLIKYQRELGVTNIYTTKNGLPIDYITGI
                     LDDDHGNLWVSTTIGVSKFDPNVGTIRTYIYEDGIQGMGETTPMKSKTGELLFGGVMG
                     LSIFHPDSIKNYDHFPPVYFTDLYLFNKQVHIGFDSLNNRTILSKSLIETDVLILNYD
                     DNVMSFDFAIIDFHSIFKWNRFAYKLEGFNKDWIYSETSRSVNNRSVTFTNLSPGEYT
                     LKVKAANSDDIWNENYASLKLIILPPWYRTIWAYLIYFILIGSIIYFTWKSQLKRITA
                     KQEVEMSKFEAQKLHEVNELKSRFFTNISHEFRTPLTLILGPVKQMIERTTDQRFRDD
                     LSMVHRNAFRLLELVNQLLDISKLESGSMKLQTVPQNIIPLLKALVLSFSSYAERKKI
                     TLQFNCDVDGIIVYLDKDKFEKIINNILSNAFKFTPSGGKIEVTVTPFFSSNTRHDHS
                     ELVSESSNDIKKPKRVQLDNNVEIKISDTGIGIPKDKLSKIFDRFYQVNSSHTREQEG
                     TGIGLALTKELIDLHKVKINVESEEGRGTIVTVNIPLGKDHLKPDEIIEQIEKQEHNK
                     PKSFENIIQVEGIGENHKNIYKEIGNDSLPLLLIVEDNSEVRKYIKDNLDSEFRVIES
                     NDGEDGWNKSVENIPDLIISDVMMPKMDGFKLCEKLKTDERTSHIPVILLTAKAAKQD
                     KLDGYELGADEYIMKPFETDELKARIKNLIQQRERIHQHFKDHGLIELNLTKINSTDK
                     KFLQSIYELVINNISEQDFTVDQLAEMINISRSVMHRKIVSLIGETPGELIRRIRINK
                     AAELIEKRFGNLSEIALEVGFNSPAYFSEAFKKQFGVTPSQYQHKFSNF"
     gene            3407..>3713
                     /locus_tag="VF870_15980"
     CDS             3407..>3713
                     /locus_tag="VF870_15980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253746.1"
                     /translation="MINHLVPSAFYLGQNYPNPFKEKTIIKYCVAFTSNVQITVYDAE
                     GKEVKKLVDEEHQPGTYQVEFSTTQCLSRKNGNLKDGYYCYRMTAGSYSSEKKWFCKN
                     "
BASE COUNT         1381 a          572 c          691 g         1069 t
ORIGIN      
        1 gctatgggtc tcaactggcg gagccgggct gcacattaat gataaaaagc aaaagcagtt
       61 caaactttat gctaatcagc ccaacaataa atattcgcta agttcaacag cagttatgtc
      121 tgtttatgaa gatagaacgg gaacaaggtg gataggtgtg cgagatggtg gattaaacag
      181 gatggaaaag ggaagtaata aatttattca ttacaagcac aaccctcttg atccaaacag
      241 tttgagtgat aataatgttt cctctatcta tgaagataaa cagggcattt tatgggtcgg
      301 aacctctaat ggagggctta acaaatttga tagaaaaaca gaaaagtttt ttaattataa
      361 acacaatcct tccaatccta aaagtataag tgataataga atattgacta tcactgaaga
      421 caattcggga aaattgtgga tcggaacaaa tttcggtggg ttgaatcagt ttaaccggga
      481 tgatgaaact tttactcatt atagattcga taaggatgat ccgaacagta ttagttcgga
      541 ttttgtttca gaattgtata ttgatctttc gggaagactg cttgtggcaa ctaaagaagg
      601 actggatgaa tttgatcaaa agaataaaaa gtttttgcac tataacctta atgaaaaagg
      661 atcagataaa attaggcaca ccagggtcac agccttatat caacaaaaga atggcattct
      721 ttggatagga acttttgata gaggattgat taaatatcaa agagaacttg gagttaccaa
      781 catctacact actaaaaatg gtttacctat agattacata acagggatat tagatgatga
      841 ccacggaaat ctatgggtaa gcacaacaat tggagtttca aaatttgatc ctaatgttgg
      901 aacaattaga acgtatatct atgaagatgg aatacaggga atgggggaaa ccactcctat
      961 gaaaagtaaa accggcgaat tacttttcgg tggtgtaatg ggactgagta tttttcatcc
     1021 tgatagtatt aaaaactatg atcattttcc gccagtttat tttaccgatc tttatttgtt
     1081 taataaacag gtacacattg gatttgatag tctaaacaat cgtaccattc ttagtaaatc
     1141 attgatcgaa actgatgtac ttatattgaa ctatgatgat aacgtaatgt catttgattt
     1201 tgccattata gattttcact caatatttaa gtggaacaga tttgcttata aactcgaagg
     1261 gttcaataaa gattggattt attccgaaac cagcagatca gttaacaata gatcagtcac
     1321 gtttacaaac ttaagtcccg gtgaatacac ccttaaagta aaagcagcaa acagcgatga
     1381 tatttggaac gaaaattatg catcacttaa actcataatc ctaccgccct ggtacagaac
     1441 tatatgggca tatctgatat atttcattct gatcggaagc attatctatt tcacctggaa
     1501 atctcaacta aaaagaataa cagcaaagca agaagttgag atgagtaaat tcgaggcaca
     1561 aaaactgcac gaagtaaatg aactgaaatc tcgattcttt actaatattt cacacgagtt
     1621 tcgaacacct ctaactctta tccttggacc ggttaaacaa atgattgaaa gaacaacaga
     1681 tcagagattt agagatgatt taagtatggt tcatagaaat gcttttcgtt tgcttgaatt
     1741 agtaaatcaa ctgcttgata tttcaaaact tgaatccggt agtatgaaac tccagacagt
     1801 tccgcaaaac atcattccgc ttcttaaagc attggtgtta tctttttcgt cctatgcgga
     1861 aagaaaaaag attactctgc aattcaattg tgatgtggat ggaataatcg tttatctcga
     1921 taaggataag ttcgaaaaga tcatcaacaa catactatct aatgcattta agtttacacc
     1981 ttccggcggt aagattgaag taactgtaac gccttttttc tcatcgaaca ctcgtcacga
     2041 tcattccgaa cttgtttcgg aatcttcaaa tgatatcaag aagccgaaac gagttcagct
     2101 tgacaataat gttgaaatta aaatatcgga taccggcata ggcataccta aggacaaact
     2161 ttctaaaata tttgatcgtt tttatcaggt taatagcagc cacacaagag agcaggaagg
     2221 aaccggtatc ggacttgcat tgaccaaaga attaatcgat ttacacaaag taaaaataaa
     2281 tgttgaaagt gaagaaggaa gaggaacaat agttactgta aatataccgc tgggtaaaga
     2341 tcatctaaaa cctgatgaaa ttattgagca gatcgaaaaa caagaacata ataaacccaa
     2401 atcatttgaa aacattatcc aggttgaagg aataggcgag aaccataaaa atatttataa
     2461 ggaaatagga aatgactctt tgcctctgtt acttatcgtt gaagataatt ctgaagtacg
     2521 taagtatatc aaagataatc tggattcaga atttagagta atagaatcca atgacggtga
     2581 agatggatgg aataaatcag ttgagaatat tcctgattta attattagcg atgttatgat
     2641 gcctaagatg gatggattta agctatgtga aaaattaaaa actgatgaaa gaacaagtca
     2701 tattcctgta attctgttaa ctgcaaaagc tgcaaagcag gataaattgg atggttatga
     2761 gctaggtgcg gatgagtata taatgaaacc atttgaaact gatgaattga aagccagaat
     2821 taaaaatctg atacagcaaa gggaaagaat acatcagcac tttaaagatc acggattgat
     2881 tgaactgaat cttacaaaga ttaattcaac ggacaaaaag tttttgcaat caatttatga
     2941 attagtaata aataatattt ctgaacagga ttttactgtt gatcagcttg cggagatgat
     3001 taatatcagc agatctgtaa tgcacagaaa aatagtatct ctaattggtg aaacaccagg
     3061 agaattgata agaagaataa gaattaacaa agctgctgaa ctgattgaaa aaagatttgg
     3121 caatttatcc gaaattgcct tagaagttgg atttaatagt ccggcgtatt tttctgaagc
     3181 atttaagaaa caattcggtg taactccctc acaatatcag cacaaattta gcaactttta
     3241 atactcgtga aataattcta aagcacgttg acaaaaaagt gtaagtaatt gacagaaatc
     3301 cggtagataa cgcctccata atatcttaca atatatccgt tcattaaaaa taattttggc
     3361 aacaaacaat ccgcttttct gtgtgatcca ttaaataaag gtaaaaatga taaatcatct
     3421 ggttccatca gcattttatt tgggacagaa ttaccctaat ccgtttaagg aaaaaacgat
     3481 aataaaatac tgtgttgcat ttacaagcaa tgttcaaata acagtttatg atgctgaagg
     3541 taaagaagta aaaaaacttg tagatgaaga acatcagccc ggtacttatc aagtagaatt
     3601 ttcaaccaca caatgtcttt cccgaaaaaa cggaaatcta aaggacggat attattgcta
     3661 ccgtatgaca gccggaagtt attcaagcga aaaaaaatgg ttttgtaaaa acg
//
LOCUS       DASWBY010000884         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_74976, whole genome shotgun sequence.
ACCESSION   DASWBY010000884 DASWBY010000000
VERSION     DASWBY010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74976"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VF870_15985"
     CDS             complement(<1..1146)
                     /locus_tag="VF870_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HEX9253747.1"
                     /translation="MDRKKILWVDDEIELLRSHIIFLSEKGYEVDTVTNGEDAITSVK
                     EKVYDIIFLDEMMAGMGGLETLAQIKNINNSIPVVMITKSEEETLMNEAIGGKISDYL
                     TKPVNPSQVLLVCKKILEGKKISGQYAAKDYLQDFNQITQALSSGLDFTEWISVYQKL
                     VNWSIELDKHREIGLEQSLNDQFKEANKEFSKFVEKNYISWLDANDKSDAPNLSPQIT
                     EKYVLKHLRDEGKSIFYFVLDCLRLDQWLVMENHLTDLFKIEKDYYYSILPTATPYAR
                     NSLFAGLYPADIEKNYPNLWVSGNDDENSMNKYEKELLQLLIDRKRIKLRNDLKYIKI
                     IDPEVGRNFEQNIHSYQNTHLTAVVVNFLDMIAHGRSDSDILKEIAPD"
     gene            complement(1215..>2746)
                     /locus_tag="VF870_15990"
     CDS             complement(1215..>2746)
                     /locus_tag="VF870_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HEX9253748.1"
                     /translation="NYDFRQEIVGRSAGFSSKSTIGLTMRWKSLFDPFFKEEDSKDKP
                     PVGNLVGKDIRLNPELKIPKLDSLGNPIAVVDSAQILDSLLFESKKPSIISRGVSFLI
                     AAMKYVFLDYEMISINFSNDNTLSKSGLKSYGTGFANFWGFSYDVNDGPTRGFMLGIS
                     SDAGPRASSGNTNLSDVTSQKNSIDFKTSRPLWEGAKIDLTWKSGWSVNKNSTINVDE
                     DGTIFVTNVNSTGTLNRSFLTIPPVFALSIFKSGINQVAELYNPQDPNSNLSQAFIEG
                     FETFPWLSKIPFLQDFANYIPRANWRITWDGLERLPLLKSLADRISLDHAYSSTYTEG
                     WKLNREGTEEIQVQKIEYGFAPLIGLNLTFGQLWGGNFSGNLKYSTRNSFDLGITTSN
                     ITENFSKDIGFTLQFSKSGFELPLFGLSLKNDIEFSLAYSSTRSAAIRYDMRAYTEEG
                     TPQDGSTRVTIEPRIKYTISAKVTLSIFYKRSSVTPEGASRIPPTKTNEAGLDVNISI
                     N"
BASE COUNT          863 a          525 c          426 g          932 t
ORIGIN      
        1 atccggagca atttctttta atatatctga atcggatctc ccgtgtgcaa tcatatccag
       61 aaaatttacc acaactgcag taagatgtgt gttttgatac gaatgaatat tctgttcaaa
      121 gtttctgcca acttcgggat caattatttt tatatacttg agatcattgc gcagtttaat
      181 tcttttccta tcaattaaaa gctgaagcaa ttctttttca tatttattca tgctgttttc
      241 atcatcattg ccggataccc acaagtttgg ataatttttt tcaatatccg ctggatataa
      301 tcccgcaaac aaagaatttc ttgcataagg agttgctgta ggcaatatag aataataata
      361 atccttctca atcttaaata aatcagtgag atgattttcc attactaacc attgatcaag
      421 acgaagacaa tcaagaacaa aataaaaaat cgatttaccc tcatcacgca gatgcttaag
      481 cacatatttt tctgttattt ggggtgaaag atttggagca tcagatttat cgtttgcatc
      541 aagccaggaa atgtaatttt tttcaacaaa ttttgagaac tctttatttg cttctttaaa
      601 ttgatcattt aacgactgct ctaaacctat ctcacgatgt ttatcaagtt cgatggacca
      661 gttaactaat ttttgataaa cgctaatcca ttcggtaaaa tcaagacctg aacttagagc
      721 ctgagtaatc tgattaaaat cctgcaagta atcttttgca gcatattgtc ctgatatttt
      781 tttaccttca agaatttttt tacaaactag aagtacctga cttggattta caggtttggt
      841 cagataatca gaaatctttc cgccgatcgc ctcgttcatc agagtttctt cctcagattt
      901 cgtgatcatc actaccggaa ttgaattatt aatatttttt atttgtgcaa gtgtttcaag
      961 tccgcccatt ccagccatca tctcatcaag aaaaattata tcgtaaactt tttctttaac
     1021 agatgtgata gcatcctcac catttgttac tgtatcaact tcgtaccctt tttcagaaag
     1081 gaaaataata tgagatctaa gcaattcaat ttcatcatcc acccaaagaa ttttttttct
     1141 atccattcta taaaaccttt ttaatttttt aatctaaagt aagaataata tttacccgta
     1201 accaatacaa aaaatcaatt tatagagatg ttaacatcta aacctgcttc atttgtcttt
     1261 gttggtggaa ttcttgatgc accttcaggt gtaacagaag atcttttata gaaaattgat
     1321 aaagtaactt ttgcgcttat cgtatattta atgcgcggtt ctatagttac ccttgttgat
     1381 ccatcctgtg gtgttccttc ttcagtatat gcacgcatat catatcttat tgcagcactt
     1441 cttgtactgg aataggcaag actaaattca atatcatttt ttaatgacaa accaaacaac
     1501 ggaagttcaa atcctgattt agaaaactgc aaagtaaatc caatatcctt agaaaaattc
     1561 tcggttatgt tacttgttgt aattcccaga tcaaatgaat ttcttgttga atactttaag
     1621 ttacctgaaa agttgccgcc ccacaattga ccaaatgtta aattcaatcc gattaatgga
     1681 gcaaaaccat attcaatttt ttggacctga atttcctcag taccttcacg atttagtttc
     1741 cagccttcag tataagttga tgaatatgcg tgatcaagtg aaattctatc agccaaagat
     1801 ttaagcagag gaagtctttc caaaccatcc caggttatcc tccagttagc gcgtgggata
     1861 taattagcga agtcctgcag gaacggtatt tttgaaagcc aggggaaagt ttcaaatcct
     1921 tctataaacg cttgtgaaag atttgaatta gggtcctgcg gattatagag ttctgcaacc
     1981 tgattaatac cactcttaaa aattgaaaga gcaaataccg gtggaatggt taagaatgaa
     2041 cggttcaacg ttccggttga atttacattt gtaacaaata tagttccgtc ttcatcaaca
     2101 tttatagttg aatttttatt aacagaccaa ccgctcttcc aggtaagatc aatttttgct
     2161 ccttcccaaa ggggtcttga tgttttaaaa tcgatactat ttttttgaga agttacatcg
     2221 cttaaatttg tattgcccga tgatgctcgc ggacccgcat cgctgcttat tcccagcata
     2281 aagcctcgag ttggaccgtc atttacatcg taagaaaatc cccagaaatt ggcgaaacct
     2341 gttccatagg attttaatcc ggactttgaa agagtattat cattactaaa gttaattgaa
     2401 atcatctcat aatctaaaaa tacatacttc atggcggcaa tcagaaaact aacaccacga
     2461 gaaattatgg atggtttctt tgactcgaat aataatgaat ccagaatctg agcagaatca
     2521 actactgcaa ttggattccc taatgaatct aattttggaa tttttaattc tggatttaag
     2581 cggatatctt ttccaacaag attacccacc ggtggtttat ccttggagtc ttcttcctta
     2641 aaaaatggat cgaataaaga tttccatctc attgttaatc caattgtgga tttactacta
     2701 aatcccgcgc tcctaccaac tatctcctgg cggaaatcat aattcc
//
LOCUS       DASWBY010000885         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_371766, whole genome shotgun sequence.
ACCESSION   DASWBY010000885 DASWBY010000000
VERSION     DASWBY010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371766"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..637
                     /locus_tag="VF870_15995"
     CDS             158..637
                     /locus_tag="VF870_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253749.1"
                     /translation="MNNENFKVSKNYFQILNIIFIALLSGQTIYFFVGLFLIQSGNIN
                     GFGGLNTMFMFVTPVVILSSILASKFIYTKQVTEFDKKLSLENKMASYRSAKIIQLAL
                     LEGANIFNISIMIITTNYFFAALFIIIIFLFFFNRPTREKFIMDYEVSAEDALKILV"
     gene            687..1913
                     /locus_tag="VF870_16000"
     CDS             687..1913
                     /locus_tag="VF870_16000"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9253750.1"
                     /translation="MKKFQIPNIYRSSFITRIKNTRKDFDPRKKDYTPTLLDFGPVRF
                     YLARHFGFCYGVENAIEIAYKTIDENPGKRIYLLSEMIHNPGVNNDLEKLGVKFLMDT
                     SGKHLVEWSELSKDDIVIIPAFGTTLEIEKKLNEIGINPYTYNTTCPFVEKVWNRSEQ
                     LGNDDFTVIIHGKSYHEETRATFSHSKTNASSVIVRDIEETKLLSSFIANERPASEFY
                     EIFKGKYSDDFDVEKDLQKIGVVNQTTMLATETQAIADLLKEVMIKKYGVENIKKYFA
                     DTRDTLCYATNDNQEATYGLLKENADFAIVVGGYNSSNTSHIVELCEEKFPTYFISSE
                     EKILSAELIKHFNIHQHKEIESENYLPQKAVVDIILTSGASCPDAVVEAVLRKVHSFF
                     PTAKDVDEVVEIYLTK"
BASE COUNT          699 a          270 c          315 g          647 t
ORIGIN      
        1 catcggaaag ctgcgagttt atttgatcaa atcttatctt aattttttct aatcgttcta
       61 ataaattcat ttcatttctc taaatttgga aagcaaatat actgaacaag ctacagattt
      121 ctaagacttc atatatcata aatcaacggt atttattatg aataacgaaa attttaaagt
      181 atctaaaaac tactttcaga tattaaacat tatatttatt gcccttttat ccggacaaac
      241 aatttatttt tttgtaggat tatttcttat tcaatcaggt aatattaatg gatttggcgg
      301 attaaatacc atgtttatgt ttgtcactcc cgttgttatt ctttcaagca ttcttgcttc
      361 aaagtttatc tatacaaaac aggtaactga gtttgataag aaactatcat tagagaataa
      421 aatggcatct tatcgaagtg cgaaaattat tcagttagcc cttttggaag gcgctaatat
      481 ttttaacata tcaattatga taattactac taattacttt tttgcagctc tttttataat
      541 cataatattt ttgttctttt ttaacagacc gactagagaa aaatttatta tggattatga
      601 agtttccgca gaagatgcat taaagatttt agtttgattt agaactttag aataactaca
      661 gatgtttatg ataaaaagat agctaaatga agaaatttca gataccaaat atttatagaa
      721 gctcatttat tacaaggatt aaaaatacaa gaaaggattt tgatccccgt aaaaaagatt
      781 atactccaac ccttttagat tttggtccgg ttagatttta tctggcaaga cactttggct
      841 tttgttacgg agtagaaaat gcgattgaaa ttgcttacaa aacaatcgat gaaaatccgg
      901 gcaaaagaat ttatttatta agtgagatga ttcacaatcc cggcgtgaat aatgatcttg
      961 aaaaactagg tgttaaattt ttaatggata ccagcggcaa acatttagtt gaatggagtg
     1021 aacttagcaa agatgatata gttatcattc cggcttttgg gacgacgctt gaaattgaaa
     1081 aaaaattaaa tgaaatcgga attaatcctt atacctataa tactacctgt ccatttgtag
     1141 aaaaagtctg gaacagaagc gaacaattag gtaatgatga ttttacagtt attattcatg
     1201 gtaaatctta tcacgaagaa acacgggcga ctttttctca cagcaaaact aatgcttcat
     1261 cagtaattgt aagagatatt gaagaaacta aattgctctc ttcttttatt gctaatgaaa
     1321 gacctgcaag tgaattttat gaaatattta agggaaaata ttctgatgat tttgatgttg
     1381 aaaaagattt acaaaaaatt ggagttgtaa accaaactac aatgcttgca acagaaacac
     1441 aggcaatcgc tgatttgctt aaagaagtta tgataaaaaa atacggagtt gaaaatatta
     1501 aaaagtattt tgccgataca cgcgatacgc tttgttatgc aactaacgat aaccaggaag
     1561 caacatatgg gttgctaaaa gaaaatgctg attttgcaat tgtggttggc ggatacaaca
     1621 gttcgaatac atcccacatc gttgaactgt gcgaggaaaa atttccaaca tattttatct
     1681 catctgaaga aaaaatttta agtgctgaac taattaaaca tttcaatatt catcagcata
     1741 aagaaattga atccgagaat tatcttccac aaaaagcagt tgttgatatt attcttacta
     1801 gtggtgcaag ttgtccggat gcagttgtag aagcagtatt aaggaaggtt cattctttct
     1861 ttccaacagc aaaagacgtt gatgaagtgg ttgaaatata tttaactaaa taatactaga
     1921 aattatatga g
//
LOCUS       DASWBY010000886         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_112549, whole genome shotgun sequence.
ACCESSION   DASWBY010000886 DASWBY010000000
VERSION     DASWBY010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112549"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            547..1587
                     /locus_tag="VF870_16005"
     CDS             547..1587
                     /locus_tag="VF870_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253751.1"
                     /translation="MKDFILFLTTFGLLTFGFGCSSGKYASSENEYTIDKESISSSQL
                     YKLLDVSVRNVSIETEETKFLVEKVCEVNDSSVTIISAPDYEEYEKAVEKFKEDYFNG
                     KVTSDDYPKRKPADTLIIAISAIQSLNYSISNPNQYSENDPEFTVPVTGLEFDFRKDE
                     YTVGFFMQMQINFYRPIIEANTSFNSSKENIWISSSALYTMGYSFIAMMIVKTIKGEP
                     INGAINELAMVKVPGPNSSNDLLSFLIFLPLLLTNAEHHILIINPITPEGTNLLGLSI
                     FAEFRTDIYAPDWIVYSPGYGIQLEFVRGKNEVKDSYYSMYLQTGLEYPYNFQFNNFQ
                     KPKLFAGLKFSF"
     gene            1599..1976
                     /locus_tag="VF870_16010"
     CDS             1599..1976
                     /locus_tag="VF870_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253752.1"
                     /translation="MKRYISAILIPCLLLQLYGCYSWRVVEKPEPDSHIKIYIKNNQV
                     IEPKNWHYENGNFIFETDKEKLKGYKAVKVETMLDQKKIYRIEEEYLNITNTSLLVAG
                     IILFVGLLVYTTAFIDWANDGFK"
BASE COUNT          709 a          316 c          299 g          691 t
ORIGIN      
        1 gtttgggctt ccaatcacat atatattatc atccttgtca attgccatag attttatctc
       61 tatatagctt aaatgttcat cagaagccgt gttaaatatc aatccgcaac ttgttaaaat
      121 aaatgaacat aaaaccaaca aaaggatata tattttcgtt tgcataatct aatccttaaa
      181 cccaatttga ttctttaaaa tcctatatgg aatataaatc caaataaaga tctacttgtc
      241 ataaaattgt caaagatttc acaaaagaat ttaaggtgca ataattctac attctgaatt
      301 cacagcccca tcctatcttc cacagctaaa ccaaaaatac cgccttcact tatttagctt
      361 attataattc tatatgttta tattttttat aatcatcctc acttaatcgt ataatcactc
      421 tttccaacct ttaatggtaa atagactttc attaattgtt aacactaaag taatttttga
      481 caactttttg acaactccac ttttgctttt tcttatcttt taagcgagta agttatgagg
      541 tctgtaatga aagattttat tttatttctg actactttcg gattactcac tttcggtttt
      601 ggttgttctt caggcaagta tgcaagtagt gaaaacgagt acacaataga taaggaatca
      661 atatcaagct cacaacttta taagttactt gatgtttcag taagaaatgt gagcattgaa
      721 actgaagaaa ccaaattctt agttgaaaaa gtatgtgaag taaatgattc atctgttact
      781 atcataagtg caccggatta tgaagaatac gaaaaggcag ttgagaaatt caaagaagac
      841 tatttcaatg gtaaagttac ttcagatgat tatcccaagc gtaaaccagc agatacctta
      901 ataatagcaa taagtgcaat tcagtcactc aattactcca taagcaatcc aaatcaatat
      961 tcagaaaacg atccggagtt tacagtccca gtaactggat tagagtttga cttcaggaag
     1021 gatgaatata ctgtgggttt ttttatgcag atgcaaatta atttttatcg accaattatt
     1081 gaagccaata caagctttaa ttcaagtaag gaaaatatct ggatatcatc ttctgcatta
     1141 tatactatgg gatattcctt tattgctatg atgattgtca aaactataaa gggagaacct
     1201 ataaatggag ctattaatga attagctatg gtaaaagttc cgggacctaa ttcaagcaat
     1261 gatttattat cgtttcttat attcttaccg cttctattaa caaatgcaga acaccacatc
     1321 ttaattatta acccgattac tcccgaaggt actaatcttt tgggattatc aatttttgca
     1381 gaattcagaa ctgatatcta tgcccccgac tggattgttt attctccggg atatggtatt
     1441 cagttagaat ttgtacgtgg taaaaacgaa gtaaaagatt cttattattc aatgtattta
     1501 cagactggtt tagaatatcc atataacttt cagttcaata atttccaaaa gcccaaactg
     1561 tttgcaggat taaaattcag tttttgagga catttaaaat gaaaagatat atttctgcaa
     1621 ttttaatccc ttgcttgctt ttgcaattat atgggtgtta tagttggcga gtagttgaga
     1681 aacctgaacc cgactctcat atcaaaattt atataaaaaa taatcaggtt attgaaccta
     1741 aaaattggca ttatgaaaat ggaaatttca tttttgaaac ggataaagaa aaacttaaag
     1801 gctataaagc agtcaaagta gaaacaatgt tagatcaaaa aaagatttat aggattgaag
     1861 aagaatatct taatattaca aatacatcat tgttagttgc tggaattatc ctatttgttg
     1921 gtcttttggt ttatactact gctttcattg attgggcgaa cgatggattc aaataattta
     1981 gttaaaataa atcaaaaaca aacgtatgtt tcttt
//
LOCUS       DASWBY010000887         5789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_371984, whole genome shotgun sequence.
ACCESSION   DASWBY010000887 DASWBY010000000
VERSION     DASWBY010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371984"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VF870_16015"
     CDS             complement(<1..668)
                     /locus_tag="VF870_16015"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9253753.1"
                     /translation="MRVPLLDLKPQYQSLKKELDEAVIKVAESQYFILGPEVSAMEKE
                     FCNYLGCKHALGVSSGTDALLLALMAIDIKPGDEVIVPTYSFFATAGVVSRLFATPVL
                     VENDPVTFNIDPKDFEKKITPKTKAVIPVHLYGQSAELDEIVNIAKSHNIIVIEDAAQ
                     AIGTQYKDGRFVGTIGDIGCFSFFPSKNLGCYGDGGLVTTNNDELAKILTIKRVHGGE
                     PKYY"
     gene            complement(692..1240)
                     /gene="rfbC"
                     /locus_tag="VF870_16020"
     CDS             complement(692..1240)
                     /gene="rfbC"
                     /locus_tag="VF870_16020"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX9253754.1"
                     /translation="MEVIKTELEGLLIIRPKIFGDERGYFFESYNQDSYRNAGIDYIF
                     LQDNISKSKKGTIRGLHYQVGNKAQGKLCKVIYGKVLDVAVDIRFGSPTFGKYFSSEL
                     SEENHTQLWIPPGFAHGFSVLSEEAIFSYKCTELYSKEHERAILFNDPSLNINWKVDN
                     PIISEKDLKAALLKDIQKDFIY"
     gene            complement(1245..2126)
                     /gene="rfbA"
                     /locus_tag="VF870_16025"
     CDS             complement(1245..2126)
                     /gene="rfbA"
                     /locus_tag="VF870_16025"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX9253755.1"
                     /translation="MSTDKKTKGIILAGGSGSRLYPITKVYSKQLALIYDKPLIYYPL
                     SILMLGGIKDVLIISNEETIPLYKQLFNSGSTIGMNINYAVQPAPNGIAEAFIIGEKF
                     IGDDNVCLVLGDNIFYGDLTFFYEGIKKNSGATIFGYQVNDPERYGIVEFDKTGNALS
                     IEEKPENPRSQYAVPGLYIFNNEVVNISKNLKPSPRGELEITDVNKEYLNRKQLRVEK
                     IGRGIAWLDTGTPEALLQASNFFGVIEDRQGLKVACIEEIAYHKGFINKVQFTDLVNS
                     IPKSLYRSYLEKILKEG"
     gene            complement(2220..3599)
                     /gene="mgtE"
                     /locus_tag="VF870_16030"
     CDS             complement(2220..3599)
                     /gene="mgtE"
                     /locus_tag="VF870_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX9253756.1"
                     /translation="MLSRLLQPEIQSLIEERQLGVLKEILTDWTPADIAELLVELSEK
                     ERVIIFRLLSRELAADTFEYMDFDTQMELLKSMGREESAAILNEMDPDDRTALFEELP
                     SSAAKQLIQLLSPDERKVAVTLLGYPENSVGRLMTPDYIAIKPSFTIQQTLDYIREFG
                     KNKETLNIVYVVDEKGKLIDDVKIKDFLLAPVDQTVADLMDENFVALHVFDDQETAVE
                     AFKKYDRVALPVVDRAGILIGIVTVDDVFDVAEEEVTEDIHKQAAVEALDEPYSTIPL
                     LSMIKKRAGWLAILFLGEMFTASAMAFFEDEIAKAVVLALFVPLIISSGGNSGSQAAT
                     LVVRAMALGEVTIKDWFFVIKRELVTGLTLGSILAVIGFLRIFAWQMASHIYGDYWLF
                     VAITVSISLVGVVIWGTLMGSMLPFILKKLGFDPASSSAPFVATLVDVTGLVIYFSVA
                     IITLSGKLL"
     gene            3733..4872
                     /locus_tag="VF870_16035"
     CDS             3733..4872
                     /locus_tag="VF870_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HEX9253757.1"
                     /translation="MFRLIVDENIAFADKAFSQFGEVKLIHGRDITNSVLMDADILIV
                     RSITTVDETILKNTPIKFVGTATIGTDHIDLEYLKQNKIAFADAKGCNAFSVAEYVVA
                     ALLKLAVKFEFSLMEKSIGIVGVGNVGSKVAAFAEALGMEVLLNDPPLKRSGDKRKFV
                     ELDKILNCDIVSLHTPLNLEGTDKTFHLLDNNNLNKLKDGVILINTSRGAVINNNDLL
                     DSIIKKNLKVVLDVWENEPSINLGLLNEVLIGTPHIAGYSYEGKVNGTKMIYESLCEF
                     LGMNKTFSFNLPEVQNSLPHIAESNKTETDIDNLVSIIYSINNDNKRMRKMITMNEEE
                     RIKYFDLLRKNYPGRREFNNYTIESNNLNKSTKAILKNLRFNLST"
     gene            complement(4865..5677)
                     /locus_tag="VF870_16040"
     CDS             complement(4865..5677)
                     /locus_tag="VF870_16040"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX9253758.1"
                     /translation="MLLIMLVGLTINLSAQSDTVKTYFPNGNLESIVIYEKNVRQGEA
                     IFYWENGNVKEKRNYVNDKVFGTVTSFYENGKMKELYSIEDGRRDGPATYYDSTGSVI
                     EDVMFENGVRKGQEFLLVGEYREEDYQKLLAQWKERQVQKEKQNGQEDLNLPPIEEDK
                     NDMDDDPAYYSSVEVMPEPIGGMNTIYKKLVYPVEAKEKKIEGTVKILAFIEKNGEVS
                     SAQVVEGIGYGCDENARLSVYYTRFKPGLQKGKRMKVQMIIPIEFKLDKETK"
BASE COUNT         1924 a         1075 c          870 g         1920 t
ORIGIN      
        1 tgataatatt ttggttcgcc gccgtggact ctttttattg taagaatttt tgcaagctcg
       61 tcattatttg ttgtaactaa tccgccatca ccatagcagc caaggttttt acttggaaag
      121 aacgagaaac atccaatatc accaattgtc ccaacaaatc ttccatcttt gtattgtgta
      181 ccgattgcct gtgcagcatc ttcaattaca attatgttat gtgattttgc aatattaact
      241 atctcatcta actcagcact ttgcccatat aaatgaactg gaataactgc tttagttttg
      301 ggtgttattt tcttttcaaa atcttttgga tctatattaa aagtaaccgg atcattttca
      361 actaaaacgg gagtagcaaa taatcttgaa acaactccag ccgtagcaaa gaaagaataa
      421 gttggaacaa taacctcatc gccgggtttt atatctatcg ccatcaaagc taatagtaaa
      481 gcatctgtac ccgaagaaac tccgagtgca tgtttacatc caagataatt acaaaattct
      541 ttttccattg cagatacttc tggtcccaaa ataaaatact gggattctgc aactttaata
      601 actgcttcat caagttcttt ttttagagat tgatattgtg gtttaagatc taatagtggt
      661 acgcgcattt tatttcctct gtgataaaaa attaataaat aaaatctttt tgaatgtctt
      721 ttaatagagc ggcttttaaa tctttctccg aaattattgg attatcaact ttccaattaa
      781 tatttaaaga tggatcatta aaaaggattg cacgctcgtg ttctttgctg tatagttctg
      841 tacatttata agagaagatt gcttcttcag aaagcacaga aaatccgtga gcaaatcccg
      901 ggggaatcca aagttgagta tgattttctt ctgataattc tgatgaaaaa tattttccga
      961 aagtcggcga cccaaaacga atgtccacag ctacgtctaa aactttacca taaatcactt
     1021 tgcaaagctt accctgagct ttattgccga cctggtaatg taaacctctg attgttccct
     1081 ttttagattt ggaaatgtta tcttgtaaaa aaatataatc tattccggca ttacgataag
     1141 aatcttgatt ataactctca aaaaaatatc cgcgttcatc accaaaaatt tttggtctga
     1201 taattaacaa tccctctaat tctgttttta ttacctccaa ctaactatcc ttcctttaga
     1261 attttttcaa gataagaacg atacaatgat ttgggaatgg aatttaccaa atcagtaaac
     1321 tgaactttat taatgaagcc tttgtgatat gcaatctctt ctatacaggc aactttcaac
     1381 ccctgtctgt cttcaatcac accaaaaaag tttgatgcct gcagtaatgc ttcaggagtc
     1441 cctgtatcaa gccacgcaat tcctcttcct atcttttcaa ctctcagttg ctttcgatta
     1501 agatattcct tgttaacatc ggttatttct aactcaccgc gtggagaagg tttaagattt
     1561 ttagagatat ttacaacttc attgttaaag atgtaaagtc ccggcacagc atattgtgat
     1621 cttgggtttt caggtttttc ttcaatagac aaagcattac cagttttatc aaactcaact
     1681 attccataac gttcaggatc atttacctga taaccaaaaa tagtagcgcc tgaatttttt
     1741 ttaatccctt cataaaagaa agttaaatct ccataaaaaa tgttatcacc taagacaaga
     1801 cagacattat catcgccaat aaatttttct ccgattataa atgcttcggc aattccgttt
     1861 ggggctggtt gtacagcata attgatattc attccaattg tactaccgct attaaacaat
     1921 tgcttataaa gcggaatggt ttcttcgttt gaaatgatta atacatcttt tattccaccg
     1981 agcatcagaa ttgatagagg ataatatatt aatggtttat cataaataag agctaattgt
     2041 ttgctgtaaa cttttgtgat aggataaagc cgagacccgg aaccaccggc taaaattatc
     2101 ccttttgttt ttttatctgt actcactttt taaaccttat tattataaat aatgctgtaa
     2161 atataaaaat aatacctaaa tcaatgttga atttaataca aattaaaatg gtattctgat
     2221 tataaaagct taccacttaa agtgataatg gcaacggaaa agtaaataac taatccagta
     2281 acatccacta aagttgccac aaacggtgcg gaagatgatg caggatcaaa tccaagcttt
     2341 tttagtataa atggaagcat tgatcccatt agggttcccc aaataactac tcccactaat
     2401 gaaatagaga cagtaatagc tacaaaaagc caataatctc cgtaaatatg tgaggccatt
     2461 tgccaggcaa aaattctcaa aaatccaata accgccagaa tagatcctag ggttaaacca
     2521 gtaactaatt ctcttttaat tacaaagaac cagtctttaa ttgtaacttc accaagtgcc
     2581 attgctctaa ccaccaatgt agccgcttgt gaacctgaat ttccgccgct ggaaataata
     2641 agtggaacga acagagctaa tacaactgct tttgctattt catcttcaaa aaaagccatt
     2701 gcagaagccg taaacatttc ccccaaaaat aaaattgcta accatcctgc tctcttttta
     2761 atcatactca atagtggaat tgttgagtaa ggttcatcca acgcctcaac tgcggcttgc
     2821 ttatggatat cctcggtaac ttcttcttca gcaacatcaa acacgtcatc aactgttact
     2881 attccaatta gtattccggc acgatctaca actggcagag caactctgtc atatttctta
     2941 aaagcttcaa cagccgtttc ctggtcatca aaaacatgca atgcaacaaa gttttcatcc
     3001 atcagatcag ctaccgtttg atctacgggt gcaagtaaaa aatccttaat tttaacatca
     3061 tcaattaatt tccccttttc atcaacaacg taaacaatat ttaatgtttc cttgttcttt
     3121 ccaaactcac ggatataatc aagggtctgc tgaattgtaa aggaaggttt gatagcaatg
     3181 taatccggag tcattaacct tccaacagaa ttttctggat atcccaacag agtgacagcg
     3241 acttttcttt catccggaga aagtagctga attagttgct ttgcagccga tgaaggaagt
     3301 tcttcaaaca gagctgtacg atcatcggga tccatttcat taagtattgc tgcgctttct
     3361 tcacgcccca tagatttaag cagttccatt tgagtatcaa aatccatata ctcaaaagta
     3421 tcggctgcta attctcttga tagcaaacga aatattatta cacgttcttt ctccgaaagt
     3481 tcaacaagga gttcggcaat atcggcgggc gtccaatcgg taagtatttc tttcaataca
     3541 ccaagttgtc tctcttcaat aagagattgg atttctggtt gtaataatct gcttagcatc
     3601 ggaaaatcct tattttagag aaaacaattt gtttttacat gttttagatt tgtattgttt
     3661 aattggcttg caaaaaataa ttgtattatg ttcaaatcac aatctaaaat ttagagatta
     3721 aagttaataa ttatgttcag attaattgtt gatgaaaata ttgcttttgc agataaagca
     3781 tttagtcaat ttggcgaagt taagctaatt cacggacgtg atattactaa ttcagtttta
     3841 atggatgctg atattttaat tgtccgttca attacaacgg ttgatgaaac gattttaaaa
     3901 aacactccaa ttaaatttgt tggaaccgca acaattggaa ctgatcatat tgatttagaa
     3961 tatcttaagc aaaataaaat tgcttttgca gatgctaaag gatgtaatgc tttctccgtt
     4021 gcagaatatg tagttgcagc attactaaaa ctagctgtta aatttgaatt ttctcttatg
     4081 gaaaaatcaa taggaattgt tggagttgga aatgtgggaa gtaaagttgc agcatttgct
     4141 gaagcactcg gaatggaggt cttattaaat gatccacctt taaaaagaag cggtgataaa
     4201 cgaaaatttg ttgaattaga taaaatttta aattgtgata tagtttccct tcatacacct
     4261 ttaaatcttg aaggcaccga taaaactttt catcttcttg ataataataa tctaaataaa
     4321 ctaaaagatg gagtcatact tattaatact tcgcgtggcg ctgtaattaa taataatgat
     4381 ttgcttgatt caatcattaa gaaaaattta aaagtggttt tggatgtttg ggaaaatgag
     4441 ccaagtataa atttgggttt attaaatgag gtgcttattg gaacaccaca cattgcagga
     4501 tattcatatg aagggaaagt aaacggcaca aaaatgattt acgaatctct gtgtgagttt
     4561 ttagggatga ataaaacttt ttcattcaat ttacctgaag tacaaaattc actcccgcat
     4621 attgctgaat caaataaaac tgaaacagat atagataatt tagtctcaat tatatattca
     4681 attaataatg ataacaaacg aatgcgtaaa atgattacaa tgaatgaaga agaaagaata
     4741 aaatattttg atttactaag aaaaaattat ccgggaagaa gagagttcaa caattacaca
     4801 attgaatcaa acaatcttaa taaatcgaca aaagctatac ttaaaaatct tcgttttaac
     4861 ttgtctactt agtttcttta tcaagtttaa actcaatcgg aattatcatt tgaactttca
     4921 ttctcttccc tttttgtaat ccgggtttaa aacgtgtgta atagactgaa agtcttgcgt
     4981 tttcatcaca gccataacca attccttcaa caacctgtgc agaagatacc tcaccatttt
     5041 tttcaataaa tgccaagatt tttacagtcc cttctatctt cttctctttc gcttcgactg
     5101 gataaaccaa ctttttatag atggtattca ttccaccaat cggttcaggc ataacttcaa
     5161 cacttgaata ataagcagga tcatcatcca tatcattttt atcctcttca atcggcggaa
     5221 gatttagatc ctcctgacca ttctgctttt ccttttggac ttgcctttcc ttccattgcg
     5281 ctaaaagttt ttgataatct tcttcacgat actcaccaac caataaaaat tcctgaccct
     5341 tacgaacacc attttcaaac attacatctt caataacact accagtagaa tcataataag
     5401 ttgcaggacc atcgcgcctt ccatcttcaa tactataaag ctctttcatt ttaccatttt
     5461 cataaaacga agtaaccgtt ccgaatactt tatcattcac ataatttctt ttttctttaa
     5521 catttccgtt ttcccaataa aatatcgctt ctccctggcg tacatttttt tcataaatga
     5581 cgatagattc caaatttcca ttcgggaaat atgttttaac cgtatctgat tgcgcagaca
     5641 gattaatcgt taaaccaact aacattatta ataacaaaga aaacttcatc taaataaacc
     5701 tggtaaattt ttgtggcaaa agataatatt taattctgag taatgagagg cataataaga
     5761 gtgataataa aaatgataat tgcaatccc
//
LOCUS       DASWBY010000888         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_526065, whole genome shotgun sequence.
ACCESSION   DASWBY010000888 DASWBY010000000
VERSION     DASWBY010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526065"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VF870_16045"
     CDS             complement(<1..989)
                     /locus_tag="VF870_16045"
                     /inference="COORDINATES: protein motif:HMM:NF015089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9253759.1"
                     /translation="MRTENKISTDVFLLTGEWSDYSGKNVLKFIGTSDELGAVEIIIT
                     NNKPVFFVDKEAILDPLTKTYLRKETKLKSFNNRPVDAVYFNTQKALTVLADTIQQSN
                     QTTYESDVDPLRRFLMEKSINSQLKITGVYEKRDSIVRFTNPAIIPCDTSPKFVIASI
                     DIETGAKTNQLYSIAVHITGKNGGHKKVFMIGEKPKRNSELISFYYNERELLSNFFLW
                     FKEIDPDIIIGWHVIGFDLLFLDNKCRELGISLDIARTNGRVSIRQRKPSGHFITITG
                     RVVIDGPTMLRASFFSFEDFKLETVAQELLGTGKTITPDENKIEEIDRLFKED"
     gene            complement(989..>1517)
                     /locus_tag="VF870_16050"
     CDS             complement(989..>1517)
                     /locus_tag="VF870_16050"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX9253760.1"
                     /translation="ENLTPNVFAFVKPIKDNSREITDGVKVILEKDLRWLRCDIKSTS
                     LLPSVMANQSAIVKKAYEAILFRNDFITEGSHTNFFAVKNNKVFTAPLSNYILSGVTR
                     KVVLELCSKNKIDFSEEYIKPGDIKTFDEFFITGTTTEITPIVQIDDWIVGDGKPGIL
                     TRTLQEKFFKAVNNI"
BASE COUNT          457 a          270 c          249 g          541 t
ORIGIN      
        1 ttatcttctt taaataatct gtcaatttct tcaattttat tttcatcggg agtaatagtt
       61 ttccctgttc ctaaaagttc ctgcgcaaca gtttcgagtt tgaaatcttc aaaactaaaa
      121 aatgaagctc gaagcatagt agggccatcg attactactc tgcctgtaat agtaataaaa
      181 tgtccgctag gttttctttg tcttatcgaa actctgccgt ttgtgcgtgc aatatccaaa
      241 ctgattccaa gctcacggca tttgttatct aaaaagagaa ggtcgaatcc aataacatgc
      301 cagccgataa ttatatcagg atcaatttct ttaaaccaca aaaagaaatt tgaaagcagt
      361 tctctttcat tataataaaa tgaaataagc tctgaatttc gttttggttt ttcaccaatc
      421 ataaaaactt ttttatggcc gccgtttttc ccggtaatgt gaaccgcaat agaataaagc
      481 tgattagtct ttgcgcctgt ttcaatatcg attgaggcaa ttacaaattt tggggaagtg
      541 tcgcagggta taattgcagg atttgtaaat cgaactatgg aatctctttt ttcgtaaact
      601 ccagtaattt tcaattgcga gttaatggat ttctccatta aaaatcttct taaagggtca
      661 acatcagatt cataagttgt ttggttggat tgctgaattg tatcagctaa aacggttaac
      721 gctttttgag tattaaaata aacggcatca acaggcctgt tattaaagct tttaagtttg
      781 gtttcttttc taagataagt ttttgttaat ggatccagaa ttgcttcctt atcaacaaag
      841 aaaacaggtt tgttatttgt aataataatt tcaacagcgc caagttcatc acttgtgccg
      901 atgaacttaa gtacgttttt cccgctgtaa tcgctccatt caccggttaa aagaaatacg
      961 tccgtggata ttttattttc agttctcatc aaatattgtt tacagcttta aaaaattttt
     1021 cctgtaatgt tctagtcagg attcctggtt tgccatcacc aacaatccaa tcatcaattt
     1081 gaacaattgg cgttatctcc gttgttgttc ccgttataaa aaactcatca aatgttttta
     1141 tatcacctgg cttgatgtat tcttcagaaa aatcaatttt atttttgctg caaagttcca
     1201 gcacgacttt tcttgtaaca ccgctgagaa tataattaga taatggggca gtaaatactt
     1261 tattgttttt gaccgcaaag aaattggtat gagatccttc ggtaataaaa tcattccgga
     1321 ataaaattgc ttcataagcc ttcttcacaa tagcactttg attggccatt acggatggta
     1381 aaagtgaagt agatttaata tcgcatctta accagcgaag gtctttttcc agaattactt
     1441 ttacaccatc tgttatttct ctgctgttat ctttgatcgg ttttacaaat gcaaaaacat
     1501 ttggagttaa gttctct
//
LOCUS       DASWBY010000889         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_263065, whole genome shotgun sequence.
ACCESSION   DASWBY010000889 DASWBY010000000
VERSION     DASWBY010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263065"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VF870_16055"
     CDS             <1..388
                     /locus_tag="VF870_16055"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9253761.1"
                     /translation="EAHKYIFTVGLGILLILTIKSVTSNHAEITFNTILTESLSNFPS
                     GHTFIATVMYLAIARYFSSSRNSRLANKYLYISAAIIIALVGISRFVGAGHTVTEVIA
                     GWSLGLCWYTFAQMFLQLDQKSFTNK"
     gene            498..>1554
                     /locus_tag="VF870_16060"
     CDS             498..>1554
                     /locus_tag="VF870_16060"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9253762.1"
                     /translation="MNELLSKTFLGNSYFDYLIALATFVGGLILIFLFKKILLVRLKK
                     WADSTDTTIDDLLVKAIQKSLIPVFYFGIFYISIKNLALSKSFENGLNVIIVIITTFL
                     VVRTIISIINYFLGSYLKNTEDSEQREKQLKGIRGLVNLVIWSIALIFMLDNLGVKIS
                     AVVAGLGIGGIAVALAAQAFLGDLFSYFAIFFDKPFEIGDFIIVGDKLGAVENIGIKT
                     TRVRAIGGEQLVFSNHDLTNSRIHNYKKMERRRVVFKLGVIYQTSSEKLKQIPKIVKD
                     IIDQQGDATFDRGHFAAYGDFSLNFEFVYYVTGADYNKYMDIQQAINLSIFEAFENEK
                     IEFAYPTQTVFVNKENFK"
BASE COUNT          516 a          216 c          272 g          550 t
ORIGIN      
        1 agaagcccat aaatatattt ttacagtagg acttggaatt ttgttaatcc taactattaa
       61 atctgttaca tcaaatcatg ctgagataac ttttaatact attcttactg aatcattatc
      121 aaactttcca agtggacata cgtttattgc aacagtaatg tatcttgcaa tcgccaggta
      181 tttcagctcg agcagaaata gcaggctggc caacaaatat ttatatattt ctgcagcaat
      241 tataattgct ttagttggca taagcagatt tgtcggagca ggccacactg taactgaagt
      301 tattgccggt tggtctttag gtttatgctg gtatactttt gcacaaatgt ttttacaact
      361 cgatcaaaaa tcatttacga acaagtaatc ttaaccttct atcaaatctg cgaatcatta
      421 ctaaaattat ttagttgcat ctgaatattt tttttatata atgtaatatg tttttcaaat
      481 aatctaagag gtatataatg aatgagcttt taagcaagac ttttctcggt aattcttatt
      541 tcgattactt aatagctttg gcaacttttg tcggaggttt gattttaatt tttctattca
      601 agaagatatt attggtgaga ttaaaaaaat gggcagactc aactgatacc acaattgatg
      661 atctacttgt taaagcgatt cagaaatctc tgattccggt tttttacttt ggaatatttt
      721 acatatcaat aaaaaacttg gcgctatcaa aatcatttga gaatggatta aatgtgataa
      781 tagtaattat aaccactttt ttggttgtaa gaacaattat atctattatt aattattttt
      841 taggaagcta tctaaaaaat acagaagatt ccgagcagag agaaaaacaa ttaaaaggta
      901 ttcgtggttt agtaaattta gtgatatggt ctattgcgct gatttttatg ttagataatt
      961 tgggagttaa aatttcggca gtggtagcag gacttggaat tggtggcatt gccgttgctt
     1021 tagcggcaca agcttttctt ggagatctat ttagttactt cgcaattttc tttgataaac
     1081 cttttgaaat tggagacttc ataatcgtcg gagataaact tggggcggtt gaaaatatag
     1141 ggataaaaac taccagagta cgtgcaatag gtggagagca acttgtattt tcaaatcacg
     1201 atttaactaa ctctcgtatc cataactata aaaaaatgga acgacgtcgt gtggttttta
     1261 aacttggagt catctatcaa acctcatctg aaaaacttaa gcaaattcct aaaatcgtaa
     1321 aggatataat tgatcaacag ggggatgcta cttttgatag aggacatttt gcagcttatg
     1381 gtgattttag tctaaatttt gaatttgttt actatgtaac aggtgcagat tataataagt
     1441 atatggatat tcaacaggca attaatttat caatcttcga agcttttgag aatgaaaaaa
     1501 ttgaatttgc ttatcctaca cagacagtat ttgttaataa agaaaatttt aaag
//
LOCUS       DASWBY010000890         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_112787, whole genome shotgun sequence.
ACCESSION   DASWBY010000890 DASWBY010000000
VERSION     DASWBY010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112787"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /gene="dnaE"
                     /locus_tag="VF870_16065"
     CDS             <1..1528
                     /gene="dnaE"
                     /locus_tag="VF870_16065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9253763.1"
                     /translation="PMEFIDDFIDRKFGRKEVKYSHKILEPILKETYGIIVYQEQVIQ
                     LANKVGGMSLADADLLRRAMGKKDLKVMEDQKLVFVDGAIKNGVDKKTAHEIFETIFK
                     FANYGFNKSHAVAYSFIAYQTAYLKAHFPAEFLAANLKNEFENTDKVTKFLDDCRKLK
                     IEVLPPDVNDPSLHFEAEAGKIKFGMSAIKNVGVPAVKEIINAKNNLGRDFKSIYDFC
                     ANTDNRIVSKRALEGLVLAGAFYSINKNRAQLFEAIEPALDYAHKIQNSKMLSSDSLF
                     GEAEDVQLKEPALPYVKPWTEKEFLAQERKVIGFYLTDHPLRRYELEYKSFSTIHLGE
                     TEDIIEKSDVRVCGVVTELKTKFDKAGRTMAFFKLDDLTGSCECLMFSKAYDEFGKFV
                     EDEEPIFAVGNLESSGDAVKMQVNKVIPMKNIANELTESLKLQISKEKISTEKLIELK
                     NILIDHEGSIPVFINLTENGSDKGKLFSLQEIRVKINSELLKLLTDLLGEESVLLKSK
                     "
     gene            1618..2445
                     /locus_tag="VF870_16070"
     CDS             1618..2445
                     /locus_tag="VF870_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9253764.1"
                     /translation="MKRSDRQEFWALILGASSGFGEATALRLANDGFNILGVHLDRQA
                     TMPNVERIINEVKSSGVEAVFYNANAADEFKRNEIISEIKSLLNGKPLVKVIMHSLAF
                     GTLKPFVPSETEQAITKAQMEMTLDVMAHSLVYWVQDVVSNNLLADSARIFAMTSSGS
                     HSAIPYYGAVSAAKSALESHVRQLSVELGSRFGASVNSIMAGVTDTPALRKIPGNLDM
                     IEIAQRKNPHGRLTTPEDVAKVISLLCKDGGEWISGGLIHADGGEDIVNYVGQGKKS"
     gene            2489..>2608
                     /locus_tag="VF870_16075"
     CDS             2489..>2608
                     /locus_tag="VF870_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9253765.1"
                     /translation="MKPITFTDDNFEKEVLQSDKPVLVDFWAVWCGPCKIIAPY"
BASE COUNT          919 a          389 c          515 g          785 t
ORIGIN      
        1 tccaatggaa tttattgatg attttattga tagaaagttt ggaagaaaag aagttaaata
       61 ttctcacaaa attctagaac caatcttaaa agaaacctac gggattattg tctatcagga
      121 acaagtgatt cagcttgcaa ataaagtagg tggaatgagt ctggcagatg cagatcttct
      181 tagaagagca atgggaaaaa aagatcttaa agtaatggaa gatcaaaaat tagtttttgt
      241 tgatggtgca attaagaatg gtgttgataa aaaaactgca catgaaattt ttgaaacgat
      301 attcaaattt gcaaactatg gatttaataa aagtcatgca gtcgcctata gttttattgc
      361 ttatcaaacg gcttatctta aagctcattt tcctgcagaa tttttagcgg caaacttaaa
      421 aaacgaattt gagaacacag ataaagtaac aaagtttctt gatgattgcc gtaagttaaa
      481 aatagaagta cttcctccgg atgttaatga tccatcattg cactttgaag ccgaagccgg
      541 aaaaataaaa tttggaatgt ccgcaattaa aaacgttggt gttcctgcag ttaaagaaat
      601 tattaatgca aaaaataatc ttggcagaga ctttaaaagc atttatgatt tttgtgcgaa
      661 tacagataac agaattgtat caaagcgggc gcttgaaggt ttggttcttg ctggagcatt
      721 ttattccata aataaaaatc gtgcacaact ttttgaagca atagaacccg cactggatta
      781 tgcacacaaa atccaaaatt caaaaatgct ttccagtgat tcattgtttg gtgaagctga
      841 agatgtgcag cttaaagaac ccgcattgcc ttatgtaaag ccctggacag aaaaagaatt
      901 tcttgcacag gaacgaaaag tgataggatt ttatttaaca gatcatccgc ttcgccggta
      961 tgaattggag tataaatcct tctcgacaat tcatttgggt gaaactgaag acataattga
     1021 aaaaagtgat gttagagttt gcggcgttgt taccgaactt aaaactaagt ttgataaagc
     1081 cggaagaaca atggcatttt tcaaacttga tgatttaaca ggaagctgtg aatgcctgat
     1141 gttttcaaaa gcctatgatg aatttggaaa atttgttgaa gatgaagagc caatatttgc
     1201 tgttggaaat ttagaaagca gcggtgatgc agttaaaatg caggttaaca aagttattcc
     1261 aatgaaaaat attgcaaacg aacttacaga aagtctaaaa ctgcaaatca gtaaagaaaa
     1321 aatctcaaca gaaaaactga ttgaattaaa aaatatttta attgatcacg aaggaagcat
     1381 tcctgtgttt ataaatctta cagaaaatgg aagtgataag ggaaagttat tttcattgca
     1441 ggaaattcgg gtaaagatca actccgaatt gttaaaatta ttaacagacc tgctcggtga
     1501 agaatcagtt ttattaaaat ctaagtaaat tcaaaattat tatttgttgt ttcactttgt
     1561 tcgattttgt attattgttc ccgttataaa acacaataca tataggaaaa tatttaaatg
     1621 aaacgtagcg ataggcaaga attctgggcg cttattttag gtgcctcatc tggctttgga
     1681 gaagcaacag cattaagatt agccaatgac ggatttaata ttctcggcgt gcacttagac
     1741 cgacaggcta ctatgcctaa tgttgaaaga attatcaatg aagttaagtc atcaggcgtt
     1801 gaagcggttt tttataatgc caatgctgct gatgaattta agagaaatga aataatatca
     1861 gagataaaat ctctgttaaa cgggaaacct ttagtaaaag ttataatgca ctctcttgca
     1921 tttggtacct taaaaccatt tgtaccaagt gaaactgaac aggcaattac taaagcacag
     1981 atggaaatga ctcttgatgt aatggcacac tcacttgttt actgggtgca ggatgttgtt
     2041 tcaaataatc ttcttgctga tagcgcaaga atttttgcaa tgacaagttc aggcagtcat
     2101 tctgcaattc catattacgg cgcggtatca gctgctaaat ctgcattaga atctcacgta
     2161 agacaattat cagttgaact tggaagtaga tttggtgcct ccgtcaattc aattatggca
     2221 ggtgtaacag atacgccggc gctccgcaaa attccaggca acttagatat gatcgaaata
     2281 gcacaaagga aaaatccgca cggaagatta actactcctg aagatgttgc aaaagtaatt
     2341 tcactgcttt gtaaagacgg cggtgagtgg atttctggcg gcttgattca tgccgacggc
     2401 ggtgaagata ttgttaatta tgttggacaa ggaaaaaaat cataagcata tcataaaaaa
     2461 ttttattatt aatttttaag gatctgaaat gaaaccaatt acttttactg atgataattt
     2521 tgaaaaagaa gttttacaat ctgataaacc tgtactagtg gatttttggg ctgtttggtg
     2581 cggcccttgc aaaataattg ctccttat
//
LOCUS       DASWBY010000891         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_333163, whole genome shotgun sequence.
ACCESSION   DASWBY010000891 DASWBY010000000
VERSION     DASWBY010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333163"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..2323)
                     /locus_tag="VF870_16080"
     CDS             complement(125..2323)
                     /locus_tag="VF870_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9253766.1"
                     /translation="MNSLSRRKFLKISGATIVTAAALAGSAKTIVNAAESYSKPKGLQ
                     IIPSYCDLCFWKCGLLAYVKDGELWKVEGNPKDPLSNGRLCPRGTGGVGAHYDKERLK
                     SPLIRKSERGEEKWVEVTWDEAIDYIAVKMNKLKADYGPESMALFSHGIGGSFIKHMM
                     RAYGSPNETAPSFAQCRGPREVGFELTFGDVVGSPERTDIENAKCIVLIGSHLGENMH
                     NTQVQEFSRAVEKHASIIVVDPRFSVAASKAKYYLPIKPGTDMALLLAWMSVIINEKL
                     YDVDYINNYGYGFKEFAAEVSKYTPEWAYIETGIDPEVIRETAIEMARYKPATLIHPG
                     RHSTWYGDDAQRSRAIALLNALLGSWGRKGGFYIPYSYSIPKYPYPPYPELKKGPLDN
                     PNGKYPFATGEQISTGIREATISGQPYPIKGWFIYGTNLMQALPNRNETIKAIQNLDL
                     MVVVDVIPSEIAGWADVVLPESVYLERYDDLNVSPFREGFVGIRQPAVNEPNDQKPNW
                     WMAKKLGEKLGLGSYFQWKNIEEYLDTRLKLAGHSIDEIKKSGIIKGPEKPIYFEDGV
                     EPEFATPSGKIEFYSTQLAEAGFDPVPRYTKHPEPPSGYFRLLYGRAPVHTFSKTQSN
                     PILADLMSENEIWINADLAERYGIKSGDRIKLKNQDGVVSNSIKVKATERIRQDCVYM
                     VHGFGQKSRQLRSTYKKGASDAELVTKYNTDPLMGGTAMNVNFVTFEMEG"
     gene            complement(2320..2769)
                     /locus_tag="VF870_16085"
     CDS             complement(2320..2769)
                     /locus_tag="VF870_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9253767.1"
                     /translation="MLNEVREDLPIYPIRTAAKLLDISVHTLRMYEKEHLILPFKKST
                     SHRLYSQSDIDRINCIRSAINDSKISINGIKTIYSMMPCWDVMNCSKEDRSKCLAYLR
                     HSDPCWAVKGGDTICANTDCRNCSVYRNSTECGSIKKFIRNKLSSLK"
BASE COUNT          792 a          609 c          438 g          984 t
ORIGIN      
        1 ttcggttatg atccaatctc tattgtaacc taccgggaca ttgttttcag ttttgcaggc
       61 aataatgcaa tcctgacagc cgacacattt acgagtatct attaccattc cgtatcttgg
      121 catcttatcc ctccatctca aaagttacaa agtttacatt catagcagtt cctcccatca
      181 acggatcagt attatatttt gttacaagtt ccgcatcgct tgcacctttt ttataagtgc
      241 ttcttagctg tctggatttt tgtccaaatc cgtgaaccat ataaacacaa tcctgtctaa
      301 tccgctctgt tgctttaact ttgattgaat tgcttacaac accatcctga tttttaagtt
      361 tgattctatc accgcttttt attccatatc tttctgccag atcagcattg atccatattt
      421 cattttcact catcaaatct gcaaggattg gattcgattg tgttttacta aaagtatgaa
      481 ccggtgcacg accatataat aatctgaaat atccggaagg tggttcagga tgttttgtat
      541 atctcggaac cggatcaaac cctgcctctg caagttgagt tgagtaaaat tctatcttac
      601 ctgatggagt agcaaattca ggttccactc cgtcttcaaa atagattggt ttttcaggtc
      661 ctttaataat gcctgacttt ttaatttcat caatcgaatg accggcaagc ttcagtcttg
      721 tatcaagata ttcttcaata tttttccact gaaaatatga tcccaatcct aacttttcac
      781 cgagtttctt tgccatccac caatttggct tttgatcatt tggttcgttg acagccggtt
      841 gtcttattcc aacaaaccct tctctgaatg gtgagacatt caaatcatca tatctttcaa
      901 gataaacgga ttccggaagc acaacatctg cccaacctgc tatctcactt ggaataacat
      961 caacaacaac cattaagtct aagttttgta tagctttaat agtttcattt ctgttcggca
     1021 atgcctgcat caaatttgtt ccatatataa accagccttt aatagggtac ggttgccccg
     1081 aaattgtcgc ttctctaatt cccgttgata tttgctcacc agttgcgaaa ggatattttc
     1141 cgtttggatt atcaagaggt ccttttttca attcaggata aggaggataa ggatatttgg
     1201 gaattgagta agagtaaggt atataaaaac cgccctttct tccccagctg cccaacaatg
     1261 catttaacag tgcaattgct ctgctccttt gtgcatcatc accataccag gttgagtgtc
     1321 tgcccggatg aataagtgtc gcaggtttat atctggccat ttctattgct gtttctctta
     1381 ttacttcagg atcaattcct gtttcaatat aagcccattc cggagtatat ttagaaactt
     1441 ctgctgcaaa ctctttaaat ccatatccat aattatttat gtagtcaaca tcatagagtt
     1501 tttcattaat aattactgac atccaggcaa gtagtaaggc catatcagtt ccgggtttga
     1561 taggcagata atattttgct ttacttgcag caacagaaaa tcttggatct acaacaataa
     1621 ttgaagcatg tttttctact gcacgggaga attcctgtac ttgagtattg tgcatattct
     1681 caccaaggtg tgaaccgatt aacacaatac attttgcatt ttcaatatca gttctttcgg
     1741 gtgaaccaac tacatcacca aaagttagtt caaatccaac ttctcttgga ccacgacact
     1801 gagcaaagct tggtgccgtt tcatttggcg atccgtaagc tctcatcata tgtttaatga
     1861 aacttccgcc aattccgtgt gagaataacg ccattgattc aggcccataa tctgctttta
     1921 gtttattcat ctttactgct atgtaatcaa tagcttcatc ccaggttact tccacccatt
     1981 tttcttcacc acgttcactc tttcgaatta aaggtgattt cagtctttct ttatcgtaat
     2041 gagcaccaac tcctcctgtt ccccttgggc aaagtcttcc attacttaat ggatcttttg
     2101 gattcccttc aactttccaa agttcaccat cttttacata tgctaataaa ccgcatttcc
     2161 aaaaacataa atcgcaataa cttggaatta tctgcaaacc ttttggtttt gagtaacttt
     2221 cagcagcatt tacaattgtt tttgctgatc cggcaagtgc agcagcagtt acaatcgtag
     2281 cgcctgaaat ctttaaaaat tttcttcttg ataatgaatt cattttaaac tcgataattt
     2341 atttcttata aattttttga tacttccaca ttcggtacta ttccggtaaa cagaacaatt
     2401 tctacaatcc gtatttgcac aaatagtatc tcctccctta actgcccagc aaggatcact
     2461 atgtcttaaa taagccaggc atttactcct gtcttcttta ctgcagttca tcacatccca
     2521 gcaaggcatc atagaataaa ttgttttaat gccatttata ctgatcttag aatcatttat
     2581 tgctgatctt atacagttta ttctatctat atcggactgt gaatagagac gatgacttgt
     2641 tgatttttta aaaggaagta taagatgttc tttctcatac attctaagtg tatgaacaga
     2701 aatgtcaagc agcttcgcag cagttcttat tggataaata ggtaaatctt ctcgtacttc
     2761 gttgagcatt tagaacacag catttaaaaa ttgatattta tagataccaa cttatttgcc
     2821 aaa
//
LOCUS       DASWBY010000892         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_333227, whole genome shotgun sequence.
ACCESSION   DASWBY010000892 DASWBY010000000
VERSION     DASWBY010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333227"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1665
                     /locus_tag="VF870_16090"
     CDS             277..1665
                     /locus_tag="VF870_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX9253768.1"
                     /translation="MFDKQVYIDRRAELKKNFKTGVILFLGNDESAMNYADNTYAFRQ
                     DSSFLYYFGLDVPNLVGIIDIDENKEILFGYEFNLEDIVWMGPQPKLAQLAEQIGVAQ
                     SETIDTLKKYLKSKISKKVKIHFLPTYRGDQTLKLADLVDENPYKLKSKFSKKLVEVV
                     VTQRSIKGEEEVAEIEYAMEIAFQMHTTAMRMAKPGVIERDIAGAIEGIALSLGSGVS
                     FPPIVSKNGQTLHNHYHGNVLKEGDLLVCDAGAESLLHYASDITRTTPVGGKFSYKQR
                     EIYEIVLTAQKNAINMIKPGVKHIDVHLTAAKIIAAGLSQIGIMKGDLNAAVKAGAHA
                     LFFPHGLGHMMGLDVHDMENLGEVYVGYDDKTKRSDQFGLAYLRLAKELQPGFVFTCE
                     PGIYFIPELIDMWKKSKKHKKFINYDKVEEYRNFGGIRIEDNILVTQNGYKVLGRPIP
                     KEVDDVEAWAKS"
     gene            1774..>2266
                     /locus_tag="VF870_16095"
     CDS             1774..>2266
                     /locus_tag="VF870_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9253769.1"
                     /translation="MKIFLSVIVLTIFLLSNINTAQSLKSPEEFLGYKVGTDYKIADY
                     ETIQKYFKHLGENSKMIVYEEIGKTVQNRNMFMAVLSSEDNIKNLSKYQNIVKRLSDP
                     RTISDLEAKQLSIDGKVVVLVTCNIHSTEIASAQMSMELCYNLITSKAPEKVNEALNN
                     VIFI"
BASE COUNT          780 a          356 c          414 g          716 t
ORIGIN      
        1 ttgaaaaagg gatgattctg gatatgggcc aacttctaag tataccattc gtattaggcg
       61 gattgtattt tatatttaga aaagaaaaag cgattgccaa taaaaatcta aagactaaga
      121 cataacgaca attattatta atttaccttg gtccctttag atcttaataa aattgatata
      181 atcatcatac catttttctt ctcttatgga aatccattct tttattccat aatttaacat
      241 cagatataat tatatatatt cttttaagga gcatttatgt ttgataaaca agtttacata
      301 gatcgcaggg cggagttaaa gaaaaatttt aaaaccggtg ttatcttatt tcttgggaat
      361 gatgaatccg caatgaacta tgcagataac acatatgcct tcagacagga tagctcattt
      421 ctttactact ttggacttga tgtaccaaat ctggttggaa taattgatat cgatgaaaat
      481 aaagaaattc ttttcggata cgagtttaat cttgaggata ttgtctggat gggtccgcag
      541 ccaaaacttg cgcagctggc agaacaaatt ggtgttgcac aatctgaaac aattgatacg
      601 ctgaaaaaat atctcaaatc aaaaatcagt aaaaaagtta aaattcattt tttgccaact
      661 taccggggtg atcaaacact taagctggca gatttagtcg atgaaaaccc atacaaactt
      721 aaaagtaaat tttctaaaaa attagtggaa gttgttgtta cccaacgttc tataaagggc
      781 gaagaagaag ttgctgaaat agaatacgca atggaaattg cttttcaaat gcatacaact
      841 gcaatgcgta tggcaaaacc cggtgttatt gagcgtgata ttgccggcgc tattgaagga
      901 attgctcttt ctttagggag tggtgtttca tttcctccta ttgtatctaa aaacggacaa
      961 acgcttcata atcattatca cggaaatgtt ttaaaagaag gcgaccttct cgtttgtgat
     1021 gcgggtgctg aatcacttct gcattatgca agtgatatta cccgaacaac tcccgttggc
     1081 ggaaaatttt cttataagca gcgcgagatt tatgagatag ttttaacagc acagaaaaat
     1141 gctattaaca tgattaagcc gggagtaaag catattgatg ttcacttaac tgctgctaaa
     1201 attattgctg ccggtttatc ccaaatcgga attatgaaag gcgatttgaa tgctgctgtt
     1261 aaagctggtg cacacgctct cttctttccg catggacttg gacatatgat gggacttgat
     1321 gtacacgata tggaaaatct tggagaagtc tatgttggtt atgatgataa aactaaacga
     1381 agcgatcaat tcggattggc ttatttgagg ttagctaaag aattacaacc aggatttgtt
     1441 tttacatgcg aacccggtat ttattttatt cctgaactaa tagatatgtg gaaaaaatct
     1501 aaaaaacata agaagtttat taattatgat aaggttgaag agtatcgcaa ctttggcgga
     1561 ataagaattg aagacaacat tcttgttaca caaaatggtt ataaggtact tggccgccct
     1621 attccaaaag aagttgatga tgtggaagcc tgggcaaagt cttgatagaa attttatagc
     1681 ttatacatag agcattattt ttatgctctg cgattaattt atatttactt aaaaaatatt
     1741 tcctttcttt atccatttag cacgggagat ggcatgaaaa tttttctatc agttatcgtc
     1801 ctcaccattt ttctactctc taacatcaat acagcacaat cattaaaatc cccagaagaa
     1861 tttttaggtt ataaagtagg cacggattac aaaattgcag actatgaaac tattcaaaaa
     1921 tattttaaac atcttgggga aaattctaag atgattgtgt acgaagaaat tggaaaaact
     1981 gttcaaaaca gaaatatgtt tatggctgtt ttaagcagtg aagataatat taaaaactta
     2041 agtaaatacc agaatatagt taagaggcta tcagacccaa gaacaatttc cgatttagaa
     2101 gcgaagcaac tttcgattga tggcaaagta gtcgttcttg ttacatgcaa tatacattct
     2161 accgaaatcg catcggcaca aatgtccatg gaactttgtt ataatttgat tacttctaaa
     2221 gcacccgaaa aagtaaatga agctcttaac aatgtaatat ttataa
//
LOCUS       DASWBY010000893         3652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_263167, whole genome shotgun sequence.
ACCESSION   DASWBY010000893 DASWBY010000000
VERSION     DASWBY010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263167"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1711)
                     /locus_tag="VF870_16100"
     CDS             complement(179..1711)
                     /locus_tag="VF870_16100"
                     /inference="COORDINATES: protein motif:HMM:NF007790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HEX9253770.1"
                     /translation="MEFGILDISVFVLFFLVVVGTSMYKSRKEETGEDYFLASRSLIW
                     PIIGISLIAANISSEQFVGMSGQSAGNVGLAIASYEWMAAITLVFVAFFFLPRFLSSG
                     IYTIPEFLQYRYNSTARSLMAFYTMVIYVGVTISAVIYSGAITIYTIFNVDIEIAVWG
                     IGIIAALYTTWGGLKAVAWADVFQGTALIIGGAIVLYFGLNAVGGLDSFFSHNADKLH
                     MVLPADHPVLPWTALVIGLWIPNIYYWGLNQYITQRTLAAKSLREGQKGIIFAAAIKI
                     IIPFVIVFPGIMAAQLYSDSMSTSDAAYPTLIKNLIPTGLKGFMLAAIAGAVISSLAS
                     MLNSASTIFTLDLYKVYVKKEASQKNLVFIGRMMTLLFVLIGCLIAPSLGDPRFMGIF
                     TYIQEFQGYISPGILAAFVFGIIFKKAPPMAGVVALLACVPVYGFLQWQFGEIAFLNR
                     MAITFGIILSLMAIITIVKPLKEPKQLPKRTDIDLTPTPQLIWFGSAVILVTLVLYGI
                     FW"
     gene            complement(1782..2657)
                     /locus_tag="VF870_16105"
     CDS             complement(1782..2657)
                     /locus_tag="VF870_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9253771.1"
                     /translation="MNEGRLVILAGGISSRMKKPVNSNLNVEEKLIEDADKKSKSMIG
                     VGNNYRPFLDYLLFNARQSGFNDIVLVVGEKDSSIKEYYGSSENNNNFNGINISYAVQ
                     PIPLGRNKPLGTADALLWGLKSKPEWKGSKFTVCNSDNLYSQEALRLMLNSPHIAALI
                     DYDRSALEFDISRIERFAVTTKDENGFLIDIIEKPTPEIIESVKSKSGFIGVSMNIFS
                     FSYDVIFPILEKVPLHPERKEKELPGAVKILADEIKESVYAYPLSEHVPDLTSKEDII
                     QVKKYLEKKYKDLLF"
     gene            complement(2654..>3652)
                     /locus_tag="VF870_16110"
     CDS             complement(2654..>3652)
                     /locus_tag="VF870_16110"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253772.1"
                     /translation="ISLRISITGSRRNDNIININLPNISRKQSFNISEPIVYTEDRDY
                     FKSSFKVLLRNSFTFSSGFECIVDGKIPINAGTSSSSALIVTWINFLARMSDQSVILS
                     PEALSRYAYEAEVLEFSEPGGMMDQYSTSFGGIIAIDFFPDTLVTRLDAKLKTFVLGN
                     SNEPKDTMYILANVKDRILLIDSKLKNVDPKFSLQNVSLDELPKFKNNLSDSEFTLLE
                     GTIKNRDITRIARTELNKKDIDHRFIGALLNDHQKILRDVLGISTPKIDGMIEAALNA
                     GAYGAKINGSGGGGCMFAYAPEDPEKVKAAIEDAGGEAFIIISDSGSREEPAEEYK"
BASE COUNT         1149 a          728 c          580 g         1195 t
ORIGIN      
        1 atttaaaatg tttactcact tttatcagtt gagccgcaga aaaatttatt caagatgaat
       61 tcaagtttaa cttataagtg cctatgtgtt tttcttcaat aaccaatatt tttcccgatc
      121 aatcgttata aaaatcggaa ttaattttat aataatatta attctgtttt ttaattcatc
      181 accagaaaat tccgtacagc accaatgtta ccaatattac tgcactcccg aaccagatca
      241 attgcggagt tggtgttaga tcaatatctg ttctcttcgg aagttgtttt ggttccttta
      301 atggttttac aattgtaatg attgccatta aagatagtat aataccaaat gtgattgcca
      361 ttctgttgag gaaagctatt tcgccaaact gccattgtaa aaacccatag accggaacac
      421 aggctaataa agctacaaca ccagccatag gcggagcttt cttaaatatt attccaaaaa
      481 caaatgccgc taagattccc ggtgaaatgt atccctggaa ttcctgtatg taagtaaata
      541 tccccataaa tcttggatca cccaaagacg gtgcgattaa gcagccaatc aaaacaaaaa
      601 gtagagtcat catcctgcca atgaaaacaa ggtttttctg tgaggcttct tttttaacat
      661 agactttata caaatccaaa gtaaaaattg tcgaagccga atttagcatt gatgcaagcg
      721 aacttatcac agcaccagca atggcggcaa gcataaatcc tttaagaccg gttggaatca
      781 agtttttaat aagggtggga taagcggcat ccgatgtgct cattgaatca ctatagagtt
      841 gtgctgccat aattcccgga aacacaatca caaatggaat tattatttta attgctgctg
      901 caaaaattat ccccttttgt ccttctctaa gacttttggc cgcaagagtt ctttgtgtta
      961 tatattgatt tagtccccag taataaatat ttggtatcca taacccgatt accaatgctg
     1021 tccaaggcaa caccgggtgg tctgcaggta aaaccatatg aagtttgtca gcgttatgac
     1081 tgaagaaact atctaatccg ccgactgcat ttaatccaaa gtaaagaaca atagcgccac
     1141 caattattaa tgctgtaccc tgaaatacgt ctgcccacgc aactgctttt aatcctcccc
     1201 aggttgtgta gagagcggca attatcccaa taccccaaac cgctatttca atatctacat
     1261 taaaaattgt ataaatagtt attgctcccg agtatatgac cgcagagatt gtaactccta
     1321 cataaataac catcgtataa aacgccatca atgaacgggc agttgagtta tatctgtatt
     1381 gcagaaattc ggggatggtg tatatgccgc tacttaaaaa tcttggtaaa aaaaagaatg
     1441 ctacgaagac tagcgtaatt gcagccatcc attcatagct agcaattgcc aaaccaacat
     1501 tgccggcact ctgccctgac attccaacaa attgttcgga tgatatattt gctgcaatta
     1561 gcgatattcc aattattggc caaataagac ttcggcttgc aagaaaataa tcttcgccgg
     1621 tttcttcctt gcgacttttg tacatacttg taccaacgac gaccaggaaa aacagcacaa
     1681 atacactaat atcaagaatc ccaaattcca taaaattttc cttttaccga ttttattttc
     1741 ttgaatgaac taaagattaa ttcagttgtt actgattaag attagaataa aagatcttta
     1801 tatttttttt ctaagtactt tttaacctga ataatatctt ctttgctcgt taaatcaggt
     1861 acgtgttctg acaaaggata tgcataaacc gattctttaa tctcatctgc taatattttc
     1921 acagctccag gtaattcttt ttcttttctc tcaggatgaa gaggtacttt ttcaaggatt
     1981 ggaaagataa catcataact gaatgaaaaa atattcatac ttacaccgat aaaacctgat
     2041 ttacttttta cactctcaat aatttcaggt gtgggttttt caattatatc aataagaaaa
     2101 ccgttttcat cttttgtggt aactgcaaat ctttctattc tggatatgtc aaattccaag
     2161 gcagaccggt cataatcaat tagagccgca atgtgaggag agtttaacat caatcttaat
     2221 gcttcctgag aataaagatt atcactgttg cacacagtga acttacttcc cttccattcc
     2281 ggttttgatt ttaatcccca caacaaagca tccgctgttc ctaaaggctt gtttctgccc
     2341 aatgggattg gctgaactgc atacgaaata tttattccgt taaaattgtt attgttttca
     2401 gaagatccat aatactcttt gattgaacta tctttttcac caacgacgag tacaatatca
     2461 ttaaatcctg attgcctggc attgaataat aaataatcta aaaatggtct ataattatta
     2521 ccaaccccaa tcatagattt tgatttttta tctgcatctt cgatcagctt ttcctcaaca
     2581 ttaaggttac tattaacggg ttttttcatt cgtgatgaaa taccgcccgc aagaataacc
     2641 aatctgcctt cgttcattta tattcctccg ccggttcttc acgggatccg gaatcggata
     2701 taataataaa agcttcacca cctgcatctt caattgctgc tttcactttt tcaggatctt
     2761 caggtgcgta ggcaaacata catccgcctc cgccggagcc gtttatttta gctccatatg
     2821 caccagcatt caaggcagct tcaatcattc catcaatctt aggagttgaa attccaagta
     2881 catctcttaa tattttttga tgatcattca gtaaagcccc tataaaccta tgatcaatat
     2941 cttttttatt taattcggtt cttgcaattc ttgtaatgtc acgattcttt attgttccct
     3001 caagcagtgt aaattcagag tctgataaat tatttttgaa cttaggcaat tcatcaaggc
     3061 ttacattctg aagtgaaaat ttaggatcta catttttaag tttactatca attaaaagta
     3121 tccggtcttt aacatttgct aaaatataca tagtatcttt aggttcattt gaatttccta
     3181 aaacgaatgt tttaagtttt gcatccagtc tcgttactaa tgtatcgggg aaaaaatcta
     3241 tagctatgat tccaccgaat gatgtggaat actggtccat cattccgcct ggttctgaaa
     3301 actccagcac ttcagcttca taagcatatc tcgataacgc ttccggcgat aagattacag
     3361 actgatcgct cattctcgca agaaaattaa tccaggtaac aataagtgca gatgaacttg
     3421 atgtccccgc attgatcgga attttaccat ccacgatgca ttcaaatccg gaagaaaatg
     3481 taaatgaatt tcttagaagt actttgaatg aacttttaaa atagtctcta tcttcggtgt
     3541 agacgattgg ttcagaaata ttgaatgatt gtttacgtga gatattaggc agatttatat
     3601 ttattatatt atcattcctt cgactgccgg taatagaaat tcttaatgat at
//
LOCUS       DASWBY010000894         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_449486, whole genome shotgun sequence.
ACCESSION   DASWBY010000894 DASWBY010000000
VERSION     DASWBY010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449486"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VF870_16115"
     CDS             <1..478
                     /locus_tag="VF870_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9253773.1"
                     /translation="KEIIEKHGGTISVESEYGRGTEFVFTLPIASSNILIVDDNKTDR
                     LLYSKILKNISPEYNVEVASDGKEAIQKILSSPPALVITDHGMPVMNGYEFVVELKKM
                     NIKGKPPVIVLSSDIDRAVINDYTELGIEFVFQKPVNLGSFKLAIEKSLQKGLSED"
     gene            complement(531..>1934)
                     /locus_tag="VF870_16120"
     CDS             complement(531..>1934)
                     /locus_tag="VF870_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9253774.1"
                     /translation="IEIPFKDSLRGKFNTWNPYERNHWGYMTAAFFAPEAYYSENIRE
                     IEFNKWSGADAKAVTDFKDMVKAFHKENIAVIMDVVYNHLSEYEFGNLKEIDREYYFR
                     LNPDGSYCAVSGCGNDLRTEAPMLRRLIVESILYWMKEYHVDGFRFDLGKLLDWQTIE
                     TIIYEAKKINPDVVFVCEPWGGGYDPVGFSLRGWGAWNDQIRNGIKGENPINGHGWIF
                     GSWYGNNSPQRIKSYVNGTLTKDSLGLFQKPDHSVNYLESHDGYTFGDFIRLALGDVK
                     PDEVIKDVDKNAKLTPTQLKLNKLGALFLFTSRGITMIHSGQEYARSKVLPLTNQNDP
                     HAGMIDHNTYDKDNEVNYINYNHAKMNQDLWDYYKGLIELRKEFKSFRRVEYNDIKFL
                     SVKDNDFALGYEVTHGDENFVVLFNAHPLKEVVWDLPKGKWEVLVNSEKAGIKQLETI
                     RGKIKIPTSSGLVLKKK"
BASE COUNT          622 a          379 c          276 g          657 t
ORIGIN      
        1 taaagaaatt attgaaaaac atgggggaac aatttccgtg gaaagcgaat acggcagggg
       61 aacagaattt gttttcactt tgccgatcgc ttcttcaaat attttaatag ttgacgataa
      121 taaaacagat cgattacttt attcgaagat cctaaaaaat atttcacctg aatacaatgt
      181 tgaagttgca tctgatggaa aagaagcaat tcaaaaaatt ctatcatcac ctcccgccct
      241 tgtaattact gaccatggaa tgccggttat gaacggatat gaatttgttg tagaacttaa
      301 aaaaatgaat ataaaaggta agccgcctgt aattgtttta agcagtgata ttgatcgtgc
      361 agttattaat gattacacag aactaggaat tgaatttgtt ttccaaaaac cggttaatct
      421 tggcagtttc aaattggcga tagaaaaatc gcttcaaaaa ggattaagcg aggattagaa
      481 ttaatttacc ttcgaggttc tattcaaaac cccgaaggtt atttttaata ttattttttc
      541 tttagaacta aacccgatga tgttgggatc ttaatttttc cacgaatagt ttcaagctgt
      601 tttattccag ctttttcaga attcactaaa acttcccact tcccttttgg caaatcccaa
      661 acaacttctt ttaacggatg ggcattaaat aaaacaacaa aattttcatc cccgtgtgtc
      721 acttcataac caagtgcaaa atcattatcc tttactgata aaaattttat atcattatat
      781 tctactcttc ggaatgattt aaattctttt ctaagttcga tcaatccttt atagtaatcc
      841 cacaaatcct gattcatctt tgcgtgatta taattaatat aattcacttc attgtcttta
      901 tcataagtgt tgtgatcaat cattcctgcg tgtggatcat tttgatttgt taaaggaaga
      961 actttacttc tcgcatattc ctgcccgctg tgaatcattg taattccgcg agaagtaaac
     1021 aaaaatagcg caccaagttt gttaagtttt aattgtgttg gagttagttt tgcgttttta
     1081 tcaacatctt taattacttc atctggtttt acatcgccaa gagctaatct tataaaatct
     1141 ccaaatgtat atccatcatg agattctaaa taatttaccg aatgatccgg cttctggaat
     1201 aatccaagcg aatcttttgt aagagttcca ttaacataac ttttaattct ctgcggtgaa
     1261 ttattaccat accagcttcc aaaaatccag ccgtgtccgt taattggatt ttcgccttta
     1321 attccatttc ttatctgatc attccatgca ccccaaccgc gtaaagaaaa tcctacagga
     1381 tcataacctc caccccaagg ctcgcaaaca aaaactacat caggatttat tttttttgct
     1441 tcatatatta tggtttcaat tgtttgccaa tctaataatt taccaaggtc aaatctaaat
     1501 ccatcaacat gatactcctt catccagtac aaaatactct caacaattaa tcttcttaac
     1561 atcggtgctt cagttcttaa atcattacca cagccgctaa ctgcacaata gcttccatct
     1621 ggatttaagc gaaaataata ttcccgatca atttctttta agtttccaaa ttcatattca
     1681 gagagatgat tataaaccac atccattatc actgcaatgt tttctttatg aaatgctttc
     1741 accatatctt taaaatccgt tacagccttt gcatccgcac cactccattt gttaaactca
     1801 atttcacgaa tattttcgga ataataggct tcaggggcaa agaatgcagc ggtcatatat
     1861 ccccaatgat tgcgctcata aggattccaa gtattaaact ttccgcgtaa agaatcttta
     1921 aatggaattt caat
//
LOCUS       DASWBY010000895         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_299452, whole genome shotgun sequence.
ACCESSION   DASWBY010000895 DASWBY010000000
VERSION     DASWBY010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299452"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VF870_16125"
     CDS             complement(<1..533)
                     /locus_tag="VF870_16125"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX9253775.1"
                     /translation="MRKLIMWNIITLDGYFEGKQNWDLSHHEKIWGPELETLSLEQLH
                     TADYLVFGRVTYEGMAAYWTKAEGENAEIAELMNKIPKLVFSNTLKSVEWNNSTLIKE
                     NASAEISRIKTQGNGDMYVFGSANLSETFINDNLFDEYRIGIVPVILGGGRPLFRQGI
                     AYRSLSLISTQQLSHGGV"
     gene            complement(562..942)
                     /locus_tag="VF870_16130"
     CDS             complement(562..942)
                     /locus_tag="VF870_16130"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9253776.1"
                     /translation="MNIFLWILQILLAAQFLWHGWIMVFPPPELLDIINANVGTWQRI
                     FIGVSEALGALGLILPGLTRTLPRLTVWSAVGLMIVAISASVYHLMRGEISSSIYTAV
                     LFVIILFVAIMRWKVKPTANKEIQ"
     gene            complement(963..1382)
                     /locus_tag="VF870_16135"
     CDS             complement(963..1382)
                     /locus_tag="VF870_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9253777.1"
                     /translation="MNNSKITIQADISANTKKVWDYYTNPEHITKWNFASDDWHCPNA
                     ENDLRVGGKLKSRMEAKDGSFGFDFEATFDEVNPGKNIIYTIADGRKVEINFEDLGNK
                     TKITTIFEAENQNPVEMQRAGWQAILDNFKIVVESNQ"
     gene            complement(1416..1778)
                     /locus_tag="VF870_16140"
     CDS             complement(1416..1778)
                     /locus_tag="VF870_16140"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9253778.1"
                     /translation="MNIKDIAANFLQLVVAGNIDEAYEKFVDMNGKHHNIYFPSGLSS
                     LQDAMKENHKQFPNKKFKIKNIIAEGNMVAVHSSISLGEMQLSVVHIFRFENGRIIEM
                     WDIGQEIPEKIPNKDGAF"
BASE COUNT          596 a          378 c          280 g          690 t
ORIGIN      
        1 acaccgccat gagaaagctg ttgtgtagaa ataagcgata aactcctgta agcaattccc
       61 tgtctgaaca aaggacgtcc gccgcctaat ataacaggta caattcctat acggtattca
      121 tcaaatagat tatcattgat aaatgtctct gaaaggttag cactgccgaa tacatacata
      181 tctccattgc cttgagtttt aattctggaa atttcagcag aagcattttc tttaatcaga
      241 gtcgaattat tccattcgac tgatttcaat gtattagaaa atacaagttt cggaattttg
      301 ttcattagtt cagcaatttc agcattctct ccttctgctt ttgtccagta agccgccatt
      361 ccttcataag ttacacgacc aaaaacgaga tagtcggctg tatgtaattg ttcaagactt
      421 agtgtttcaa gttccggtcc ccaaatcttt tcatgatgag atagatccca gttttgcttt
      481 ccttcaaaat aaccatcaag tgtaatgata ttccacatta ttagttttcg catataaatt
      541 tctttcaatt tttaaattga tttattgaat ttctttattg gctgtgggtt tcactttcca
      601 ccgcataatg gctacaaata aaattattac aaaaagaaca gcagtataaa ttgaactgct
      661 aatttcacca cgcattaaat gataaacgga agcactaata gcaacaatca tcaaaccaac
      721 tgcagaccaa acagttagac gaggcaatgt gcgggttagt cctggtaaga taagtccaag
      781 tgctccaagt gcttcagaaa ccccaataaa aatacgctgc caggtaccaa catttgcatt
      841 tattatatct aacaattcag gaggtggaaa caccataatc cagccatgcc ataaaaattg
      901 agcagcaagt aaaatttgta atatccacaa aaaaatattc ataatttttc tctctattaa
      961 tttcattgat ttgattcgac aacaatttta aaattatcaa gaattgcctg ccaaccggct
     1021 ctctgcattt caactggatt ttggttttca gcttcgaata tcgtagttat ttttgtttta
     1081 tttcccaggt cttcaaaatt aatttctacc ttccttccgt cagcgattgt atatataata
     1141 ttttttccag gatttacttc atcaaatgtt gcttcaaagt caaatccgaa actgccatct
     1201 ttagcctcca ttctgctttt aagttttccg ccaactctta aatcattttc tgcatttggg
     1261 caatgccagt catcagatgc aaagttccat tttgtaatgt gttcaggatt tgtatagtag
     1321 tcccaaactt tttttgtatt tgcagaaata tctgcttgaa tagttatttt agaattattc
     1381 attactgact tccttctatt tttaatttat aagttttaaa atgctccgtc tttatttgga
     1441 attttttccg ggatttcctg accgatatcc cacatctcga taattcttcc attttcaaat
     1501 ctaaaaatat gaacaactga taattgcatt tcaccaagac ttatacttga atgtacagca
     1561 accatatttc cttcggcaat tatattttta attttgaatt ttttattcgg aaactgttta
     1621 tggttttctt tcattgcatc ttgtaacgaa gaaagaccgg atggaaaata aatattatgg
     1681 tgtttaccat tcatatcaac aaacttttca taagcttcgt caatatttcc ggcaacaacc
     1741 aattgtaaaa aatttgcggc gatatcttta atgttcatat tttaaccttt aaacgctttt
     1801 tccaaatcag caatattaaa cttcttcata ggtaagaatg tttgtgtaag ccgatcaatt
     1861 tgttctcttg tacctttact catcatttta cacaggattg ctggtgatat ttgccaggat
     1921 actccgaatt tatctttgca ccag
//
LOCUS       DASWBY010000896         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_405836, whole genome shotgun sequence.
ACCESSION   DASWBY010000896 DASWBY010000000
VERSION     DASWBY010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405836"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VF870_16145"
     CDS             <1..1155
                     /locus_tag="VF870_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9253779.1"
                     /translation="IINDNEIEKVIDKKQTEYQIRWLYSSDNNDIANYYKQIINGASF
                     DSLFKIQLNDSVLLDDRQLTSSLFNIYLKNPVFAQIIDTLKPGVISNPIHTNDGWYII
                     KIDNIWKSLITNQTEYEKLKYESEQAITKSKLDILSDEYVKSLFVDENPIIKRDAFNL
                     LRSYLGKYVLAPEKYSEWGLDDKLDAALGNLGLNRSDKYPGLTLVECKSKNISLDEFI
                     IWYRVREQYLKFIKDDLAGFSKSLEDLIWLMVRDKLVTNQAFQKGYDKSDWVVRQSDW
                     WKQKICYSAYLNELANSITLDSEEIKLADEKNRSQSELLSEKLSKKILYKVLELKKNY
                     KITVNKELLDDIKVSSENDKKAIDMYIVKRGNLVPRQAFPTIDNEWVNWE"
     gene            1162..>2045
                     /locus_tag="VF870_16150"
     CDS             1162..>2045
                     /locus_tag="VF870_16150"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9253780.1"
                     /translation="MKTFSQIITLILLTYVLTGCNNNNQNSRDAILYWCSNNSDEIKF
                     AKDITENWNKNNPNQQVKYQPVPEGRSSEEIILAAVVGNTTPDIYSNMWQGDVEAYAQ
                     AGVLIALDTLEGFNEFLYDRCDSSVIEEIKSLDGHIYQIPWKINPIMLIYNVNLINEL
                     ELQYPPKTYSEFFNASKKFQKDFDGDGYVDRWFGYCEVEVTWWQRFFDFYPLYLAASG
                     GAPLVKNNQAAFNNKYAVEVFSFLQEMYKKNYFSKEKLSARQDVFLSGVTATRFTGPW
                     EIAHAEKFKPKGFEYEYA"
BASE COUNT          744 a          292 c          375 g          634 t
ORIGIN      
        1 attataaatg ataatgaaat cgagaaagtc attgataaaa aacaaacgga atatcaaata
       61 agatggcttt actcaagtga taataatgat atcgcaaatt attataagca gattataaat
      121 ggtgcttctt tcgattcatt gttcaaaatt cagcttaatg attcagtgtt actagatgac
      181 agacaactta caagcagtct cttcaatatt tatttaaaga atcctgtctt cgcccaaatc
      241 attgatactc ttaaacctgg agttatttca aacccaattc atactaatga tggatggtat
      301 ataatcaaaa ttgataatat ctggaaaagt ctgataacaa atcagacgga gtatgagaaa
      361 cttaaatatg aatcagaaca ggctattaca aaaagtaaac tagatattct atcggatgag
      421 tatgttaaaa gtctttttgt agatgaaaat cctattatca aacgtgatgc atttaatttg
      481 ctcagatcat atctgggaaa atatgtcctt gccccagaaa aatattctga atgggggtta
      541 gatgacaaat tggatgctgc acttggcaat cttgggttaa atcgtagtga taaatatcca
      601 ggattaacat tagttgaatg taaatcaaag aatatttctt tagatgaatt tataatttgg
      661 tatcgggttc gtgagcaata cctaaagttt ataaaagatg atttggccgg attctcaaaa
      721 tccttggaag atcttatctg gttgatggtt agagataagt tggtaactaa ccaagctttt
      781 caaaaaggat atgacaaaag cgattgggtt gtcagacaat ctgattggtg gaaacaaaaa
      841 atatgttaca gtgcttatct taatgaacta gctaattcaa ttactttaga ttctgaggaa
      901 ataaaattag ctgatgaaaa gaatagatct caatctgaat tattaagtga aaagctttca
      961 aaaaagattc tttataaagt ccttgagctg aaaaagaatt ataaaattac tgtgaataaa
     1021 gaattacttg atgatattaa agtatcttct gaaaacgata aaaaagctat tgatatgtat
     1081 atagtcaaaa gaggaaattt ggtgccaaga caagcattcc cgacaattga taacgaatgg
     1141 gttaattggg aataaaacat aatgaagact ttttcacaaa ttataacact aatcttactc
     1201 acttatgttt taaccggttg caataataat aaccagaatt cgcgtgatgc gatattgtac
     1261 tggtgttcta ataattctga tgaaataaaa ttcgcaaaag atattactga aaactggaac
     1321 aaaaataatc caaatcaaca agttaaatac cagcctgttc ctgagggacg atcgagtgaa
     1381 gaaattattc ttgctgcggt tgtaggaaac acaaccccgg atatttattc aaatatgtgg
     1441 cagggtgatg tagaagccta tgctcaggca ggcgtgttaa tcgcactcga tacgcttgaa
     1501 ggttttaatg aatttcttta tgatcgatgt gatagttcag tcattgaaga aataaaatca
     1561 cttgacggcc acatctacca gataccttgg aagattaatc ctataatgct gatatataat
     1621 gttaatctta taaatgaatt ggaattgcag tatccgccta aaacatacag tgaattcttt
     1681 aacgcctcaa aaaaatttca aaaagatttt gatggcgacg ggtacgtgga tagatggttc
     1741 ggctattgcg aagttgaagt tacatggtgg caaagatttt ttgattttta tccattatat
     1801 ctggcagcat ccggtggcgc cccgttagtt aaaaataacc aggcagcctt taataataaa
     1861 tatgcagttg aagttttttc ttttcttcaa gaaatgtata agaaaaatta tttttctaaa
     1921 gaaaaacttt ctgcaagaca ggatgttttc ttaagcggtg taacagcaac cagatttaca
     1981 gggccctggg agatagctca cgctgaaaaa tttaaaccca agggttttga atatgaatat
     2041 gcatt
//
LOCUS       DASWBY010000897         6593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_526399, whole genome shotgun sequence.
ACCESSION   DASWBY010000897 DASWBY010000000
VERSION     DASWBY010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526399"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VF870_16155"
     CDS             <1..227
                     /locus_tag="VF870_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253781.1"
                     /translation="EKDTGPHTFQIKGKSVNCLHCGNTQFLQKQILLNTPGMTFFSLD
                     WANKTASVLICTKCSQILWFNEEPEVILEM"
     gene            425..1084
                     /locus_tag="VF870_16160"
     CDS             425..1084
                     /locus_tag="VF870_16160"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX9253782.1"
                     /translation="MISILTIIVLSILTIIINFILVENSSSEISKGKPIVQTDRIKEA
                     LLVIDIQEGITGKASSDDFFTSHSEMLIKTVNQIIDTSARYNIPIVYVKNEISNPLIN
                     ILNNSLAKGSPGAELDSRLKILSNYVINKDKADAFSNPLLDSILITNGINKLVFTGLD
                     LAQCVNSTILAAVNRNYKICLISDAVITKPDSLKNGILEKFRQSGLEIISSEEYIKRI
                     N"
     gene            1297..1866
                     /locus_tag="VF870_16165"
     CDS             1297..1866
                     /locus_tag="VF870_16165"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9253783.1"
                     /translation="MNPKIIVIISIAYLYGFFEYYLSLRQRQKTKIVRKDDRGSLWLL
                     LISITIGYLLSFSIAATKIGRIYHWNLLFTLGLFIIGIGLVIRVISIWQLRQYFTYSV
                     SKVENHLLIDKGFYKFIRHPGYLGQLLIFTGIAISLSNWLAVMLMLLSTLVGYTNRIR
                     VEEKFMIEQMGDIYSNYQKRTKKLIPKIY"
     gene            2004..2699
                     /locus_tag="VF870_16170"
     CDS             2004..2699
                     /locus_tag="VF870_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEX9253784.1"
                     /translation="MVFIHSEKHRTQHIGWLRAAVLGANDGIVSIASLVVGVAAAQTA
                     TNDILIAGVAGLVAGAMSMAAGEYISVSSQADTEKADIARERMELEKNVESEKEELAS
                     IYVKRGLDSELALKVAEQLMAHDSIGAHARDELGISEILTARPIQAALSSAGTFSVGA
                     IMPLLAVLVFPKSYLIPAVSLTSLFCLALLGMLSAKIGGSPIVKATLRVTFWGALAML
                     ITAGIGALFGIAV"
     gene            complement(2715..2918)
                     /locus_tag="VF870_16175"
     CDS             complement(2715..2918)
                     /locus_tag="VF870_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253785.1"
                     /translation="MRASFRSFLTIRKEHYNNSFTELNNLDMIDALDKCIDVALMWHR
                     INAAVKAQLLQSNVHNFLKIYGG"
     gene            3419..4357
                     /locus_tag="VF870_16180"
     CDS             3419..4357
                     /locus_tag="VF870_16180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9253786.1"
                     /translation="MKTRLVSIQIIYLFFLTTFYYGQSNNVSEFEGLKEINSTQLFFK
                     VIGNGEPLLIVHGGPGLGHDYLFEPFKQLADNNTLIFFDQRGCGRSEQLNTSDSVDMN
                     TLVDDIEQIRKEFNISKLNLAGQSWGALISIEYTVKYPENVSKLLLLEPAPGSNEYLG
                     EFQQTIMNRLSQNDKESIAELSQNPKLKFDPILFDEFMSLRFKGYSLDTTFVSKMHMD
                     YFDSLRVLKFFSSAAKFGPYLMNFNLYEKMKSINCPTLIIHGENDPIPNDAIKKMGSN
                     IKNAELHIIKDSGHFVHIEKPKVYFELIRSFLKKSE"
     gene            4572..5282
                     /locus_tag="VF870_16185"
     CDS             4572..5282
                     /locus_tag="VF870_16185"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HEX9253787.1"
                     /translation="MKALVLGASGATGKLVVQQLIKNNIEVRIVVRESALIPSYVSEN
                     NTIEIVKGNINDFGITKFKDLLKDCDSIICCLGHNISFKGIFGPPHKLVYNTVVRIIE
                     AVKSLKLNPKYILMSTTAYTNNKIGEVNTVREKIVFSLLYVLLPPHKDNMHAANHLVY
                     KVGLQSSIEWVVVRPDSLFDEENVSNYEIHNKKIRSPIFDAGKTSRINVAHFMIELLL
                     NEELWNKWKFKMPVIYNR"
     gene            5346..6278
                     /locus_tag="VF870_16190"
     CDS             5346..6278
                     /locus_tag="VF870_16190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9253788.1"
                     /translation="MKRITISAIVMILGFSSIGYGQKEFIDVNGKKMEIFIAGLENNQ
                     KDKPVIVFENGMATKLDKWKTIIEEVSKTSAVFSYNRPRIGESENDSLPPTTKHIVDN
                     LRKMLLEKGLKPPYLLVGHSFGGAYIRSYASYYPDEIAGLIFVDPVDFTKKKGDGDLP
                     YLEIGLTQHQIDSMFAKPYKDFVENLYADMPNFYVEEVKILRQLTKTEFKECDSIPLP
                     DVPVHFIQAGGYPKTPDDRGATSFDKLKLFRIDNNLKMKRWLALLNPLQYGKYFYCSN
                     SGHYMQEDEPETIISSIELALNDYDKIQKAKKIN"
BASE COUNT         2326 a          965 c         1206 g         2096 t
ORIGIN      
        1 cggagaaaga cacaggcccg catacatttc aaataaaagg aaaatcagtt aattgtctac
       61 attgcggaaa cacacagttt ttgcaaaagc aaatattatt gaatacgcct ggaatgacat
      121 tcttttcttt ggattgggca aacaaaacag catcagttct tatatgcaca aaatgcagtc
      181 agattttatg gtttaatgaa gagccggaag taatacttga gatgtaaaaa aaagatactc
      241 ctggaaaagg cacaggctta tattgcgaat tataaaatat tgtttgctaa tttttaatgg
      301 atactgaaaa ataaggtaag tttaaataag taattgtgaa cgatccctta taagtattag
      361 ttaataattt tttctcatat ttagtagctg tgttgatata aaaggctgga ttatgaagaa
      421 gataatgatt tcaattttaa ccattattgt tctgagtatt ttgacaatca taataaattt
      481 tattttggtt gagaatagtt catccgaaat atcaaaaggt aaacctattg tacaaacgga
      541 tcgcataaaa gaagcattgc ttgtaataga tatacaggaa ggcataaccg ggaaagcatc
      601 cagtgatgat tttttcacat cacactccga aatgttaatt aaaactgtta atcaaattat
      661 tgatacatct gccagatata atatccccat cgtatatgtt aagaatgaaa tatcgaatcc
      721 tttaataaat atccttaata attctttggc taaagggagt cctggtgccg aactggattc
      781 aagattaaaa attttatcaa attatgttat aaataaagat aaagctgatg cttttagtaa
      841 tccactactc gatagcattc ttataacaaa tggtattaac aaacttgtat ttacaggact
      901 tgatttagca cagtgtgtta acagtacgat attagctgca gtaaatagga attataaaat
      961 atgtttgata agtgatgcag taattacgaa accagattcg cttaaaaatg gtatacttga
     1021 aaaattcaga caaagtggac ttgaaattat atcaagtgaa gaatatatca agagaataaa
     1081 ttagttatga aaaataactg tgcccaacag ccattaaaga gtttataata tatcgactaa
     1141 tatctttaat ctgccacagg cggacaggca agaggatata gaagtattgt tcaaactgtt
     1201 gatgcggctt gctaaaagag ttacttaaca ttgtgatggc tgtgccagaa attttactaa
     1261 caacaataaa tacaataaaa aatatcgagt atctaattga atcctaaaat aatcgttatt
     1321 ataagtattg cataccttta cggatttttt gagtattatt taagtttacg acaaagacaa
     1381 aaaacaaaga ttgtccgcaa agatgataga ggaagcttat ggctgctgct tatatccata
     1441 acaattggtt atttgctttc tttttctatt gctgctacaa aaattggcag aatttatcat
     1501 tggaatcttt tgttcacgtt aggattattt atcattggga ttggattagt tattagggtt
     1561 atttccattt ggcaactcag gcaatatttt acatattcgg tttccaaagt tgaaaatcat
     1621 ttactaattg ataaagggtt ttataaattc attaggcatc ccggttattt aggtcaattg
     1681 ctaattttta ctggcattgc tatttcatta tcaaattggt tagctgtaat gcttatgctg
     1741 ctatcgactc ttgttgggta tacaaatcga attagagttg aagaaaaatt tatgattgaa
     1801 cagatgggtg atatatattc caattatcag aagagaacaa aaaaactaat accaaaaatt
     1861 tattaaaata aatatactct aactgctttt ataagcataa aaggaaattc gaaaataaat
     1921 tatatggtat tcgtgtttaa gataacaatt cgataggatt caagagttgt agagtaaata
     1981 atattcccat tttaggagtt taaatggttt ttatacatag cgaaaaacat cgtactcaac
     2041 atattggttg gttacgagca gctgtcttgg gagccaatga tggaattgta tcaattgcaa
     2101 gcctcgttgt tggagttgcg gcagcacaaa ctgcaacaaa tgatatttta atagccggag
     2161 tcgctggtct tgttgctggg gcaatgtcaa tggctgctgg tgaatatatt tcagttagtt
     2221 cgcaagcgga tacagaaaaa gccgatatag cacgcgaacg aatggaactg gaaaaaaatg
     2281 ttgaatcgga aaaagaagaa cttgcatcaa tttatgtaaa acgtggatta gattcagagc
     2341 ttgctttaaa agttgccgaa caattaatgg cgcatgactc aattggtgct catgctagag
     2401 atgagcttgg tatttctgaa atacttacag cgcgtcctat tcaggccgca ttatcttcag
     2461 ctggaacttt ttcagtaggt gccattatgc ccttattagc tgtactagta tttccaaaat
     2521 cttatttgat accagccgtg tctttgacct cattattttg tctggcttta ctgggaatgc
     2581 tttctgctaa aattggtggt tcccccatag ttaaggctac cttacgtgtt acattttggg
     2641 gtgcacttgc aatgttgatt acggcgggta ttggagcttt gtttggaatt gctgtttaaa
     2701 tatttataat ataatcatcc tccataaatc tttagaaaat tatgaacgtt tgattgaagt
     2761 aattgtgcct taacggctgc atttattcta tgccacatta atgctacatc aatacattta
     2821 tctaaagcat caatcatatc aagattattt aattctgtaa aagaattatt ataatgttct
     2881 tttcttatcg taagaaaaga acgaaaagaa gcacgcactt taaaaataat tgctagaatt
     2941 ttctttcatt acgctgaggc gaatgaactc gcccgcaaag ccgggctcaa acagcattcg
     3001 cctttatacg ctgcattaca ggaaattctt taacgcaatt cttttaatgt gcaaacacca
     3061 gagcattaaa aaaagatttt atcgcttaag gcttcgttca acaattcttc gggcgctgag
     3121 cttttgatgt caattcaaat tcatttttta atcagttgtt ttgaggagtg gtggtgaaga
     3181 tgaaagagga aatggagaag aagaagagag attcacagaa gttaaactga agaattatta
     3241 aatgcattgc aataagtttt attgatattt ttaatttgat tgtataagtt tgaagcaggt
     3301 tgcatataaa atctgtatac aaaatgatgt acgggcagta aattatattt tatatccctg
     3361 gtgtttcttt aagcggcagg tatttttttc cgcgagaatt aagcaaaaag aggtgagtat
     3421 gaaaactaga cttgtctcaa ttcaaattat ttatcttttc tttcttacaa ctttctatta
     3481 cggtcaatcc aataatgttt ctgagtttga gggtttaaag gaaataaatt ccactcaatt
     3541 atttttcaaa gtaatcggga atggtgaacc actgctaatc gtacacggtg gaccgggact
     3601 tgggcacgac tacttatttg aaccttttaa acaattagca gataataaca ctttaatatt
     3661 ttttgatcag cgaggctgcg gaagatctga acagttaaat accagcgact ccgttgatat
     3721 gaatacttta gtagatgaca ttgaacaaat ccgtaaagag tttaacataa gtaagcttaa
     3781 tcttgccggt caatcttggg gtgcattgat aagcatagag tacactgtta aatatcccga
     3841 aaatgtttct aaacttttac tgctagaacc agcaccaggc agcaatgaat atcttggtga
     3901 gtttcaacaa acaattatga atcgtttatc acaaaatgat aaagaaagta tagcagaatt
     3961 atcgcaaaat ccgaaattaa aatttgatcc gattctcttt gatgaattta tgagcttaag
     4021 gttcaaaggt tattcactag atactacttt tgtgtcaaaa atgcatatgg actattttga
     4081 tagtttaaga gtgttaaaat tcttttcaag cgctgcgaaa ttcggaccat atttaatgaa
     4141 ttttaattta tatgaaaaaa tgaaaagcat aaattgtcct acgcttataa tacacggtga
     4201 aaatgatcca ataccgaatg atgctattaa aaagatggga tcaaatataa aaaatgcgga
     4261 attgcacata attaaggata gcgggcattt tgtgcacata gaaaagccaa aagtatactt
     4321 tgagttaata cgatctttct taaagaagag tgaatagcac taaatggtaa ttgaataatt
     4381 aaagaatagt tgaatgatga aataatttac aggagtattg ataatcaaat aataaaagat
     4441 tttattattt attttttctt aagggattta attaatagac tgctgcgaca aaaatagaaa
     4501 ttaagcgtaa agctgggtta ggcaccatgt cattaggcat cgtataaagt atttattcat
     4561 tgtgaggata tatgaaagca ttagttctgg gtgcaagtgg tgcgacgggc aaattagttg
     4621 tacaacagct tatcaaaaat aatattgaag ttcgaatagt cgtaagagaa tctgcattaa
     4681 tccccagcta tgtgtcagag aataatacaa ttgagatagt aaaagggaat ataaatgact
     4741 ttggaattac aaaattcaaa gacctgctaa aagactgtga ttcaattatc tgctgtcttg
     4801 gacataatat aagttttaag gggatttttg gcccgccgca taaactagtt tacaatacag
     4861 ttgtgagaat aattgaagct gtaaagtctc ttaaactgaa ccccaagtac atcttaatga
     4921 gcacaacggc atatacaaac aacaaaattg gagaagtaaa cactgtcaga gaaaaaatag
     4981 tattctcgtt attatatgtc ctattacctc ctcacaaaga taatatgcac gctgcaaatc
     5041 atcttgttta caaagtgggc ttacaatcca gtattgaatg ggtagtggtt cgtcctgatt
     5101 cgctttttga tgaagaaaat gtgagtaatt acgagataca taataagaaa ataagaagcc
     5161 caatatttga tgccggcaaa acaagcagaa taaatgttgc tcattttatg attgagttat
     5221 tgttgaatga agaattatgg aacaagtgga aatttaaaat gccagtgata tataatcggt
     5281 aatattaagt agtgtaataa caaagtataa atatcatcta aggaatatag ttaatgagaa
     5341 atgaaatgaa acgaataaca atttcagcaa ttgtaatgat tttaggattt agcagtattg
     5401 gttatggaca gaaagaattc atcgatgtga atggaaagaa gatggaaata tttatcgctg
     5461 gattagagaa caaccagaag gataaaccag taattgtatt cgaaaatgga atggctacta
     5521 agttggataa atggaaaaca ataattgaag aagtttctaa aacatcagcg gtattttctt
     5581 ataaccgtcc aagaattgga gaatccgaaa atgatagtct tccaccgaca acaaaacata
     5641 tagttgacaa cttaagaaag atgcttttag aaaaaggtct taaaccgcct tacctattag
     5701 tggggcattc tttcggaggc gcgtatataa gaagttatgc atcctattat cctgatgaaa
     5761 ttgcggggct tatttttgta gatcccgtag atttcacaaa aaagaaggga gatggagatc
     5821 ttccatattt ggaaattggt ttaacacaac atcaaattga ttcaatgttt gccaaacctt
     5881 acaaagattt tgttgaaaat ctttatgctg atatgccaaa tttctatgtg gaggaagtta
     5941 aaatacttag gcaactaact aaaacggaat ttaaagaatg tgatagtatt cctctgccgg
     6001 atgttccagt tcattttatt caagcgggtg gctacccaaa aacacccgat gacagaggtg
     6061 ccacaagctt cgataaattg aagcttttta gaattgacaa taatttaaaa atgaaacgat
     6121 ggctggctct tctaaatccg ttgcagtatg gtaaatattt ttactgttct aattccgggc
     6181 attatatgca agaagatgaa ccggaaacaa taatttcaag tatcgagtta gctttgaatg
     6241 actatgacaa aattcaaaaa gcgaaaaaaa taaattaata gtcagaacac acataacccg
     6301 caaatcaacc ggacgccctt tatatttaag taaaacaaaa agtgcaatag tctaaccatc
     6361 tattgcactt ttcaattttg tccgctctat cctctggaac tcctttcata ggtcagctgg
     6421 ccgccaggca ggtgaaaaat caaggcgatt cttaggatca ccttttttat tgctgcttaa
     6481 tcatacttgc taatctgcaa tcgattcttc ttgacttaat caaaaattaa tcgctattta
     6541 caagtagaga atttgtaatt tatcacaaat tttgaaatgt catcgtaatt ctc
//
LOCUS       DASWBY010000898         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_300070, whole genome shotgun sequence.
ACCESSION   DASWBY010000898 DASWBY010000000
VERSION     DASWBY010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300070"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1002
                     /locus_tag="VF870_16195"
     CDS             112..1002
                     /locus_tag="VF870_16195"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9253789.1"
                     /translation="MKIIFTILLAVVLMQSFLLTQTKDETKTLLIRCDDLGMSHAVNA
                     AFKELMESGLKFSASVMFPCAWYQEAVDILKANPQITVGIHLTLNAEWRNYRWGPISG
                     KDKVPSLVDENGYFFPSRAKFFANNPKPEEVEIELRAQIERAMNTGLKITYMDYHMGT
                     AVDKPELRKIVEKLAGEYNLGISRYFGEIDTPLMYAVPIPEKQDSLFKLVENLEPDKI
                     NLLVCHIGKDQPELQAMIDMNSFGLPEMSKHREAELKALITAQQQNLFDSYSIKLINY
                     SDLIKQIGLGNMKSPIDTGY"
     gene            1252..1680
                     /locus_tag="VF870_16200"
     CDS             1252..1680
                     /locus_tag="VF870_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253790.1"
                     /translation="MKTLIIVLIAFVLVPVALPQTVLSQEQKSGAVNNLTEGIKSTNY
                     GLRTSSADVLFDLIDDGYLKSDDASKAMIPLLVMLEKGQTDEERISAALALFNLGNSI
                     GIYRLRGVAIFDDKERVSKICKNLYYSYHKLHGTEYLIDF"
     gene            1898..>2166
                     /locus_tag="VF870_16205"
     CDS             1898..>2166
                     /locus_tag="VF870_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9253791.1"
                     /translation="MKYRKLGKSDVEVSEISLGCWTMGGLNWVNGTPNGWANVDEKEI
                     SQAINYAIDHGVNHFDNADVYGNGRAERMLARILDKRTNNFIIAT"
BASE COUNT          749 a          322 c          382 g          713 t
ORIGIN      
        1 cggagaaatt tttgtgttaa gttaccagga ctggcaaatc cattatgttg ttgatgaatt
       61 aaaaaagaaa gttaaaatcc ttaatatcat ttttgttgat aggaaaacac tatgaaaata
      121 atttttacaa ttttacttgc tgttgttttg atgcagtcat ttttacttac tcaaacaaaa
      181 gatgaaacca aaactctctt aattcgttgt gatgatcttg gaatgtctca tgctgttaat
      241 gcagccttta aagaattaat ggaatctgga ttaaaatttt ctgcatctgt tatgtttcct
      301 tgtgcatggt atcaggaagc tgtggatatt ttaaaagcta atccgcaaat aaccgttggc
      361 attcatttaa cattaaatgc agaatggaga aattatcgtt ggggaccaat ttccggtaaa
      421 gataaagtcc catctctggt agatgaaaat ggatatttct ttccatctcg tgctaagttt
      481 tttgctaata atcccaaacc agaagaagtt gagattgaat tacgagcaca gattgaaaga
      541 gcaatgaaca ctggattaaa aataacatac atggattatc atatgggcac tgctgttgat
      601 aaacctgagc taagaaaaat cgttgaaaaa cttgccggcg aatataattt aggtatctca
      661 agatattttg gggagataga tactccgctt atgtatgcag tcccaatacc tgaaaagcag
      721 gattctttgt ttaagcttgt tgaaaattta gaaccagata aaattaactt gcttgtatgc
      781 cacattggaa aagatcagcc tgaattgcag gctatgattg atatgaattc ttttggattg
      841 cctgaaatga gcaaacaccg tgaggctgaa ttaaaagctt taataaccgc acaacaacaa
      901 aacctttttg atagttatag tatcaaattg attaactatt ccgatttgat aaaacaaata
      961 ggattgggaa atatgaaatc acccattgat acaggatatt aaattatctc ctctaaaaaa
     1021 ttggattaat atcttttgtt tagtgtttaa ttatatccaa aaattaacgt cgaaggataa
     1081 acggttaatt ttgattatgg tcaaaaatta atattttttt catcataact tttcttccta
     1141 aaattttact gtcagcaatc agaaattacc actcataaca acttagtagc caacctttgt
     1201 tttaaaatta tcttagcggt gtagttttaa taatttaaaa aggagcaaaa aatgaaaact
     1261 ctgattattg tgttaatcgc atttgtactt gtaccggttg cattaccgca aaccgtgctg
     1321 tctcaggaac aaaaatctgg cgccgttaac aacctgacgg aaggaatcaa atccactaac
     1381 tatggtcttc gtacaagttc cgcagatgta ttatttgatt tgattgatga tggatatctt
     1441 aaaagtgatg atgcatctaa agcaatgata ccattattgg ttatgcttga gaaaggacag
     1501 actgatgaag agagaatttc tgctgcattg gcattgttca atttgggaaa tagtattggt
     1561 atttaccgct taagaggagt tgcaattttt gatgataaag agagagttag taaaatttgc
     1621 aaaaacttat actatagtta tcacaagctg catggcacag aatatctcat agatttttaa
     1681 ttttagttaa atacaatgtg ttatctctcc taacgtcagg ttaagaataa tgatttttaa
     1741 cctgctgcaa tttttatatt caattattta atatttcctt tcttaaccaa cagttcgatt
     1801 caattctaaa acagtaaaat attaattatt gttaattaga gttcaatatc ctccaattct
     1861 tttatatttt tgtttacaca tttaagaggg aaataaaatg aaatacagaa aacttggtaa
     1921 atccgatgta gaagtatcag aaattagtct tggctgctgg actatgggcg gacttaattg
     1981 ggttaacgga acgccaaacg gatgggctaa tgttgatgaa aaagaaatct cccaagccat
     2041 caattatgcc attgatcacg gagttaacca ttttgataat gctgatgttt atggaaatgg
     2101 cagagcagaa agaatgctgg cccgaattct tgacaaacga acaaacaatt ttataatagc
     2161 tacaaa
//
LOCUS       DASWBY010000899         5535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_406484, whole genome shotgun sequence.
ACCESSION   DASWBY010000899 DASWBY010000000
VERSION     DASWBY010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406484"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="VF870_16210"
     CDS             <1..1499
                     /locus_tag="VF870_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HEX9253792.1"
                     /translation="RVRKARKTIVELLVENHPQDCLICVRNKNCELQDLSEQYGIREH
                     RYAGETKSHAIDISSASMERDPAKCILCGRCVRVCHEVQKVGAIDFTKRGFTSIVTTP
                     YNKGLNVSDCILCGQCIIVCPTAALREKSSVKEVANAIYDRKKYCIAQVAPAVRASLG
                     EEYNMPLGTDVTGLLVTGLRRLGFKKVFDTNFAADLTIMEEATELINRVQNGGSLPMF
                     TSCCPGWVKYVEMNRPEVLEHVSSCKSPHEMEGAVLKTYYAKKMGIDPSDIYVVSIMP
                     CTVKKYESNRAELSENAMPDVDAVLTSRELVRFFKIAGIDFNDLPESEFDNPLGESTG
                     AAAIFGTSGGVMEAALRTAYFKMTGTEPEHLEFEEIRGMAGVKEATIKIGELDVNIAV
                     VNGIGNVDPILDQVQNGTSKHHFIEVMACPGGCINGGGQPIHQKPEKVMKRIKVLYKI
                     DENAKTRRSHENESVKTLYKEFFGEPNSHKAHEILHTEYFDKKKILKN"
     gene            1600..3612
                     /locus_tag="VF870_16215"
     CDS             1600..3612
                     /locus_tag="VF870_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEX9253793.1"
                     /translation="MNHAIVSTITDRCKRCYSCIRECPAAAIRVIDAQAKVIEERCIA
                     CGHCVKVCSQDAKQIFSEIDDTYQLLQSGNAIAIVAPSFAASFPDEYKKVPAALRKLG
                     FTKVIETAFGADLISNDYMEVIKADNDKTVISSACPAVVSFIQKYYADLVPNLAQVVS
                     PMIALGRYLKKNLGEDVNIVFIGPCVAKKHEALDEDVVGVIDSVLTFTELRQMFDENG
                     INVSELEDSDFDEPHAMMGKAYPLAGGLIKTIDISGDILEKDIIVVEGKKKVLEIIEE
                     IANNHINAKFTDILFCEGCISGPAIDTTLNYYARREKVINYIDEKINNVDKRVWKSNL
                     YNARKLNLKRTFRVDNQRRPYPTEEKIKEILAQTKKYSVKDELNCGACGYPTCREYAV
                     AIAKDLAEKEMCLPYVLDELKIAYDNLSDTEEQLRVAEKLASIGQLAAGVAHEINNPL
                     GTILLYASMLKRDLEKIYNDDQRTEDLELIVEEANRCKNIVANLLNFARQGKLNLKEF
                     DLTETLREVLKPFTLNPVYRQIEFKFDILDNSYSITGDEEQLKQVFINIIKNACDAMI
                     ETDHAKELKVNLSSDNGYVKIEITDTGNGIPKENHSKVFTPFFTTKSMGKGTGLGLAI
                     SYGIIKMHKGNITFTTEIGKGTAFKVLLPRHQVYQTNEGSEGAKAL"
     gene            3609..4016
                     /locus_tag="VF870_16220"
     CDS             3609..4016
                     /locus_tag="VF870_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9253794.1"
                     /translation="MRTKRKILLVDDDLDLLEQNKLLIESKGYEVITANSGKEGFEVF
                     KKTRPDACVVDLIMEEHDSGFILCYRIKKDEYGKNIPVFILTSATYDTGFKFGASTSE
                     EKEWIKADALLNKPVIIEEFVQKLEAFFERQKS"
     gene            4205..>5535
                     /locus_tag="VF870_16225"
     CDS             4205..>5535
                     /locus_tag="VF870_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9253795.1"
                     /translation="MNTYKPKVLIVDDEKGLRIGAQRLLASEGYDVSTAENGTEGIKL
                     GTETEFDLAIIDLKMPDFDGLKVLQQIKKKYPHTICFIATAYASYETAVEATRIGAHS
                     YIPKPFTPEELLSNLKDGYQKRLINLEAQKWQKEREERLLEVAFEKTRMNTIVNSITD
                     GVLVVNRDGLAVLYNPAALRFLELPVISVEEFILDKLRPEILVLFNKLVNDASTEHKS
                     YTIQIELKSNREFFIEVTAAAVRYPDENIAGVVFVFKDITSHKKIEFVKSQFVSMVSH
                     ELKAPIAAVYGFLKLFNDDSIKLTPEQRKEYEKRSMIRLDGLLKMVNDLLDISRMELK
                     TVQREIKKVCLHEIISSILELFQVEISKKNIQVVFDKDNSAHCINADADEITRLFTNV
                     ISNAIKYNSDNGIININLYQADNYLVTEIKDSGIGLKPEEKQKLFTEFFRA"
BASE COUNT         1972 a          854 c         1110 g         1599 t
ORIGIN      
        1 caagagtacg caaagcaaga aagacaatag tagaattgct tgtagaaaat catccacagg
       61 attgtttgat ctgcgtgcgc aataaaaatt gcgaacttca ggatctttcc gaacaatatg
      121 gaattcgcga acatcgttat gctggtgaaa caaaatctca cgcaatagat atttcaagtg
      181 catcaatgga acgcgatcct gcaaaatgta ttctttgcgg cagatgtgtt cgtgtttgcc
      241 acgaagtaca aaaagtcggt gcaatagatt ttacaaaacg tggtttcact tctatagtaa
      301 ctacacctta taataagggt ttaaatgtta gcgattgcat attatgcgga caatgtataa
      361 tagtatgtcc aactgctgca ttgcgagaaa agagctctgt taaagaagtt gccaatgcaa
      421 tttatgatag aaaaaaatat tgtatcgctc aggttgcacc tgcagtgcgt gcttcacttg
      481 gtgaagaata caatatgcct cttggtactg atgtaacagg attgcttgta accggtttaa
      541 gaagattagg atttaaaaaa gtattcgata caaactttgc tgctgatctt acaattatgg
      601 aagaagcaac agaacttatt aatcgtgttc agaatggcgg aagcttacca atgtttacaa
      661 gctgctgtcc tggctgggta aagtatgttg agatgaacag accggaagtt ttggaacacg
      721 tttcaagctg caaatctcct cacgaaatgg aaggtgcagt tttaaaaact tactacgcaa
      781 agaaaatggg tattgatcca agtgatattt atgttgtttc aataatgccc tgcacagtca
      841 aaaaatatga atcaaatcgt gcagaacttt ctgaaaatgc tatgcctgac gttgatgccg
      901 ttttaacatc aagggaactt gttagatttt ttaagattgc aggaattgat tttaatgatc
      961 tgccggaaag tgaatttgat aatccgcttg gcgaatcaac aggtgcggct gctatttttg
     1021 gaacaagcgg cggagttatg gaagctgctt tacgtactgc ttactttaaa atgacaggaa
     1081 ccgaacctga acatcttgag tttgaagaaa ttcgtggaat ggctggagtt aaagaagcaa
     1141 caataaaaat cggtgaactt gatgttaaca ttgcggttgt taatggaatt ggaaatgttg
     1201 atccgattct tgatcaggtg cagaatggaa cttcaaaaca ccattttata gaagtaatgg
     1261 cttgtcccgg tggatgtata aatggcggcg gtcagcctat tcatcaaaaa ccagaaaagg
     1321 ttatgaagcg tataaaagta ctttacaaga ttgatgaaaa tgctaaaacg agaagatcgc
     1381 acgaaaatga atctgtgaaa acattgtata aagaattttt tggtgaaccg aattcacaca
     1441 aggcgcacga gattcttcat acagaatatt ttgataagaa aaagatttta aagaattaga
     1501 aataagaatg ttattctgag tcccgactta tcgggacgaa gaatctgcta tacaaaacag
     1561 attcctcact tcgttcggaa tgacaattat gagaacataa tgaatcacgc aatagttagc
     1621 acaataacag atagatgcaa aagatgttac tcttgtatta gagaatgtcc tgctgcagct
     1681 atacgcgtta ttgatgcaca ggcaaaagtg attgaggaaa gatgcattgc ctgcggacac
     1741 tgcgttaaag tctgctcaca ggatgcaaaa caaatttttt cagaaattga tgacacatat
     1801 caacttcttc aatcaggaaa tgctattgca atagttgctc cttcttttgc ggcttcattt
     1861 ccggatgaat ataaaaaagt accggctgct ttaagaaaac ttgggttcac aaaagttata
     1921 gaaacagcgt ttggcgctga tcttatctcg aatgactata tggaagtgat taaagccgat
     1981 aatgataaaa cagttatcag ctccgcttgc cctgctgttg taagttttat tcaaaaatat
     2041 tatgcggatt tagtccccaa tcttgcgcaa gtggtttccc caatgatagc gcttggcagg
     2101 tatttaaaaa aaaatcttgg tgaagatgtt aacatagttt ttattggtcc ttgcgttgca
     2161 aagaaacacg aagctctgga tgaagatgtt gttggtgtta tagattctgt cttaacgttc
     2221 acagaattaa gacagatgtt cgatgaaaat ggaattaacg ttagtgaact tgaggacagt
     2281 gattttgatg aaccgcatgc tatgatgggt aaagcatatc cgctcgcagg tggattaatt
     2341 aaaactatag atattagcgg cgatattctt gaaaaggata tcattgtagt tgaagggaag
     2401 aaaaaagttt tagagataat tgaagagatt gccaacaacc atataaatgc aaagttcacg
     2461 gatatattat tttgcgaagg ctgtataagc ggacctgcaa ttgatacaac attaaattat
     2521 tatgcaagac gtgaaaaagt aataaactac atcgatgaaa aaattaataa tgttgataaa
     2581 cgtgtttgga aaagcaatct atacaatgca agaaaactaa atctgaagag aacattcaga
     2641 gttgataatc agcgaagacc atatcccacc gaagagaaga ttaaagaaat attagcacag
     2701 actaaaaaat actctgtaaa agacgaatta aattgcggag cgtgcggtta tcctacctgc
     2761 agagaatatg cagttgcaat tgcaaaagat cttgcagaga aagaaatgtg cttaccttat
     2821 gttctggatg aattaaaaat agcttatgat aatctaagcg atactgaaga acagctaaga
     2881 gttgctgaaa agcttgcatc aatcgggcag cttgcggcgg gagttgcaca cgaaataaat
     2941 aatccgcttg gtacaattct gctttatgcc tcaatgttaa aaagagattt agaaaaaatt
     3001 tataacgatg atcagcgaac tgaagattta gaattgatag ttgaagaagc caacagatgt
     3061 aaaaatattg ttgctaatct tttaaatttt gcacgacagg gaaaattaaa tctaaaagaa
     3121 tttgatctta cagaaacatt aagagaagtt ttaaaaccat ttacactgaa tcctgtttac
     3181 aggcaaatag aatttaaatt tgatatttta gataatagtt attcaataac aggagatgaa
     3241 gaacaattaa aacaggtgtt cataaatatt attaagaacg cttgcgatgc tatgattgaa
     3301 actgatcatg caaaagaatt aaaagtaaat ttatcatccg ataatggtta tgttaaaatt
     3361 gaaataactg atacaggaaa tggtattccc aaagaaaatc atagcaaagt ctttacacct
     3421 ttctttacta caaaaagtat gggcaaaggg acaggattgg gattagctat ttcttacggc
     3481 ataattaaaa tgcataaagg aaacattaca tttacaactg aaattggaaa aggtactgcg
     3541 tttaaagtcc tacttccaag acatcaagta tatcaaacaa atgaaggaag cgaaggagcg
     3601 aaagctttat gagaacgaaa aggaaaatac ttttagtaga tgacgattta gatcttcttg
     3661 aacaaaataa attattaatt gaatctaaag gttacgaagt aattacagca aacagcggca
     3721 aagagggatt tgaggttttc aagaaaacaa ggcctgatgc ctgtgtcgtt gatttaatta
     3781 tggaagagca tgattcaggt tttatacttt gttacagaat taaaaaagat gaatacggaa
     3841 aaaacatccc tgttttcatt cttacatctg caacttatga tactggtttt aagtttggcg
     3901 catcaacatc tgaagaaaaa gaatggataa aggctgatgc attattaaac aaaccagtta
     3961 taattgaaga gtttgttcaa aagcttgaag cattttttga aagacagaag agttagattc
     4021 tttatgtcat cttgagcgaa gacgaaagat gactttagat tatttatttg tcacacttcg
     4081 acttcgctca gtgtgacaca aaatgtcatt gcgaatcccc gattcatcag ggtgaagcaa
     4141 tctgtaaaat cagcggattg cttcgtcatc ccttcggtct tcttcgcaat gacgttaagg
     4201 aaaaatgaac acatacaaac caaaagttct gattgttgat gacgaaaaag gtcttcgcat
     4261 tggtgcacaa aggctgcttg ccagtgaagg ttatgatgta tcaactgcag aaaacggtac
     4321 tgaaggaatt aaactaggta ctgaaacaga atttgatctt gcaataatcg atttaaaaat
     4381 gccggacttt gatgggctta aagttctgca gcaaatcaaa aaaaaatatc cacacactat
     4441 ttgttttata gcgacagctt atgcaagcta tgaaacagct gtagaagcaa cacgcatagg
     4501 agcacacagt tacatcccaa aaccttttac acctgaagag ttgttatcta atctaaagga
     4561 tgggtatcaa aaaagattaa taaatcttga agcgcaaaaa tggcagaagg aacgtgaaga
     4621 acgtttgctt gaagttgctt ttgaaaaaac aagaatgaac acaattgtta actcaattac
     4681 agatggggtt cttgttgtca acagggatgg gcttgccgtt ctttataatc ctgcagcttt
     4741 aagattttta gaattacctg ttatttctgt ggaagaattt attctggata aacttcgtcc
     4801 ggaaattctt gtgcttttta acaagcttgt taatgacgca tcaacagagc ataaatctta
     4861 tacgattcaa atagagttaa aatctaacag ggagtttttt atagaagtta ctgccgctgc
     4921 tgttcgttat cccgatgaaa atattgccgg tgttgtgttt gtatttaaag acataacctc
     4981 acacaaaaaa attgaatttg taaaatctca attcgtctct atggtatcgc acgaacttaa
     5041 agctccaatt gccgcagtgt atggattttt aaaattgttt aatgatgatt caataaaact
     5101 aacgcccgag caaagaaaag aatatgaaaa acgttcgatg ataagattgg acggcttgct
     5161 taaaatggta aatgatcttc ttgacatttc acgaatggaa ttaaaaactg ttcaacgtga
     5221 gataaaaaaa gtttgtctgc acgaaattat ttcttctata cttgaactat ttcaagttga
     5281 aatcagcaaa aaaaatattc aggttgtttt cgataaagat aattctgcgc actgcataaa
     5341 cgctgatgct gatgaaatta cacgtctgtt tacgaatgtt attagtaatg ctataaaata
     5401 taacagcgat aatggaataa taaacattaa tctttaccag gcagataact atcttgttac
     5461 agaaattaaa gacagcggaa ttggattgaa gccggaagaa aagcaaaaac tctttacaga
     5521 attcttccgt gcaaa
//
LOCUS       DASWBY010000900         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_334151, whole genome shotgun sequence.
ACCESSION   DASWBY010000900 DASWBY010000000
VERSION     DASWBY010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334151"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /gene="purH"
                     /locus_tag="VF870_16230"
     CDS             complement(<1..1154)
                     /gene="purH"
                     /locus_tag="VF870_16230"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX9253796.1"
                     /translation="MKKLALLSVSDKSKIVEFANGLVKNGYLIIATGNTAKVLSENKI
                     DVTEISSITGFPEIFDGRVKTLHPKIFGGILFRRDNYSDIKQATENSIETIDIVCVNL
                     YPFVKTVANPKSSLDEIIENIDIGGPSLVRASAKNYKFVSILTNPDQYDSFLDELNAG
                     SISEETRKKLAVAAYSHTANYDTHIANYLENKFSLPVTHIRVNEKLSQSLRYGENPHQ
                     NAGIYGNFEEYFSVFHGKEISYNNILDLVAAVELCEDLGETACTIIKHNNPAGAAIGK
                     NPFEAYIRALKCDPVSSFGGIVAFNKEVDIETATELNKIFLEIVSAPSFSDGALEILK
                     KKKDRRLVIQKKSVLVEGKTFRSIPGGVLAQDSDSISLKEDELKFVTVKKP"
     gene            complement(1193..1798)
                     /gene="purN"
                     /locus_tag="VF870_16235"
     CDS             complement(1193..1798)
                     /gene="purN"
                     /locus_tag="VF870_16235"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX9253797.1"
                     /translation="MKIAVFVSGRGSNLNAILNFPDLKKIIEIIAVVSDKIDCKAFDI
                     AREFGIQTFTIGDGEGKIAQNKITSTLKKLDVNFIVLAGYLKLIPTELIEKFENRIIN
                     IHPALLPSFGGKGMYGLNVHRAVFNSSAKVSGATIHMVDKTYDTGKIVAQKCVDISDV
                     KSPEEIAERILKIEHQLLPEVIKAFAEDRVKFESDRIVIIK"
     gene            complement(1875..>2860)
                     /locus_tag="VF870_16240"
     CDS             complement(1875..>2860)
                     /locus_tag="VF870_16240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9253798.1"
                     /translation="AANLVGITADKELGLTYVNKTVKKGKLSKIDAQYYLSQLYSRVI
                     VDHPAAKELLSNLVRRYPKNLLFNFSLAWVEYELNDLTNAEKHLRFVIEADDDLYPFV
                     VSNSHYLLGNVFFTRNQFDSARVYYQNYLKLSINDDYKGYANYQIGLCYEISGNRKEA
                     VKYYEKSSDGNADIEEDLFAERKGDEFKDRKLTEDEIDLIKFSNLIGQNKLAAAKDSL
                     KDFIDQKTNTNDVKAEALLYLSQICYKQKKYQESLDYAVQCLKVEIKNEQWVHAYAYY
                     FGAWSSFQQKKFIDAKLFLLQINDMDDYDFRNSLENKIYTLQRLLPEERNK"
BASE COUNT          970 a          533 c          370 g          987 t
ORIGIN      
        1 ggtttcttaa cagttacaaa ttttaattca tcttctttta aagatatgga atcagaatcc
       61 tgagcaagta ccccacccgg aattgaacga aatgtttttc cttcaactaa aacagatttc
      121 ttttgaatta cgagccttct atcttttttc ttcttcaaaa tttccaaagc gccgtcacta
      181 aaagatggag cacttacaat ctctaaaaat attttattca attcagtagc tgtttcaata
      241 tcaacttctt tattaaaagc cactattccg ccaaaagaag aaacaggatc acatttcaat
      301 gctcgaatat aagcttcaaa tggattctta ccaatagcag caccagcagg attattatgc
      361 ttaattattg tgcacgcagt ttctccaaga tcttcacaaa gttcaactgc cgcaactaga
      421 tcaagaatat tattgtaaga aatctctttc ccatgaaata cagagaaata ttcttcaaag
      481 tttccgtaga taccagcatt ctgatgcgga ttttctccgt aacgtaaaga ctgactcagt
      541 ttttcattaa ctcgaatatg tgttactgga agcgagaatt tattttctaa ataatttgca
      601 atgtgagtat cataatttgc agtgtgcgaa taagccgcga cagcaagttt ctttctcgtt
      661 tcttcagaaa tacttcccgc atttaattca tctagaaaag aatcatattg atctggattt
      721 gtaagaattg aaacaaactt atagtttttt gcagaagctc ttactaaact cggtccgcca
      781 atatcaatat tttcaattat ctcatccaaa gaagattttg gatttgcaac agttttaaca
      841 aatggataca gattaacaca aactatatcg atggtttcaa tggaattttc agttgcttgt
      901 ttgatatcag aataattgtc ccttctaaaa agtattccgc caaatatttt tggatgcaaa
      961 gtttttaccc gtccatcaaa tatttctgga aaacctgtaa tcgaactaat ttcagttaca
     1021 tctattttat tttctgaaag aacttttgct gtgttgccgg ttgctattat taaataacca
     1081 tttttcacta agccatttgc aaattctaca attttagatt tatcagaaac acttagtaac
     1141 gctaacttct tcaattcaaa ttcctatttt gtaagaagat aaaaaataca tcttatttta
     1201 ttattacaat tctatctgat tcaaacttaa ctctatcttc agcaaaggcc ttaataactt
     1261 ccggcagcag ctgatgttca atttttaata ttcgctctgc tatctcttcc ggacttttca
     1321 catcagaaat atcaacacac ttctgagcaa caattttccc cgtgtcataa gtcttatcaa
     1381 ccatatgaat tgttgcacca ctcacttttg ccgatgaatt aaatacagca cgatgaacat
     1441 tcaatccata cattcctttt ccgccaaaag aaggaagcaa cgccggatga atattaatta
     1501 ttctgttttc aaatttttca atcaattcag ttggaataag tttaagatat ccggcaagca
     1561 caataaaatt aacatcaagt tttttcaaag tagaagtaat tttattttga gctatcttac
     1621 cttctccatc accgatcgta aaagtttgga ttccaaattc ccgtgcaata tcaaatgcct
     1681 tgcagtcaat cttatcgcta acaactgcga taatttcaat tatttttttt aaatcaggaa
     1741 aatttaaaat agcatttaga ttagaacctc tgccagatac aaaaactgca attttcaatt
     1801 aaaaatacct gaaatgttga cgaaaattag ctaatcaata aaaaagaagc aatgaaagaa
     1861 atgctgatat taaattattt attacgttct tccggtaata atctctgcaa agtgtaaatc
     1921 ttattttcta aagaatttct aaaatcgtaa tcatccatat cgtttatctg taaaagaaag
     1981 agttttgcgt caataaattt tttctgctga aaactactcc aggcaccaaa ataataagca
     2041 taagcatgaa cccattgttc atttttaatt tcaactttaa gacactgtac tgcataatca
     2101 agtgattcct gatacttttt ctgcttataa caaatttgag ataaataaag taaagcttct
     2161 gctttcacgt catttgtatt agttttctga tcgataaaat ctttaagcga atctttggca
     2221 gcagctaatt tattttgccc gatcagattt gaaaatttaa ttaaatcaat ttcatcttca
     2281 gttaattttc tatctttaaa ctcatcgcct tttcgttctg caaataagtc ctcttctata
     2341 tcagcattac catcagaaga tttctcataa tattttacag cttcttttct attaccggaa
     2401 atttcataac acaaaccaat ctgataatta gcgtatcctt tgtaatcatc attaatactt
     2461 agttttaaat aattctggta atatactctg gcagaatcaa attggttacg agtaaaaaaa
     2521 acattaccta aaagatagtg tgaattagac accacaaaag gatataaatc atcatcagct
     2581 tcaattacaa atcgtaaatg tttttctgca tttgtaaggt cattcaattc atattcaacc
     2641 caggccaaag agaaattaaa taacaaattt ttcggatacc ttctaaccaa attgctcaat
     2701 aattcttttg ctgcaggatg atcgacaata actctggaat aaagttgaga caaataatac
     2761 tgtgcatcaa ttttagagag cttgcctttc ttaacggttt tattaacata agttaaccct
     2821 aattctttat cagcagtaat accaacaaga ttagcagccc
//
LOCUS       DASWBY010000901         2501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_300565, whole genome shotgun sequence.
ACCESSION   DASWBY010000901 DASWBY010000000
VERSION     DASWBY010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300565"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VF870_16245"
     CDS             complement(<1..726)
                     /locus_tag="VF870_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9253799.1"
                     /translation="MNGDKIIEINSLTKDFKDLRAVNSLNLNVFEGDVFGFLGPNGAG
                     KSTTIRMLLSLIKPTEGSIKLFNKTLEKNRIEILKRIGAIVEKPDFYGYLSAYKNLEI
                     LGRISGKEISKNRIMEVLELVGLSKRDNSKVKTFSHGMKQRLGLGQALLHNPDLIILD
                     EPTTGLDPQGMKEIRDLILHLSKVEKKTIFLSSHILYEVELVANRMIIINKGTTKVEG
                     YVKDLLNAKRLKVSFEVNDEEKTT"
     gene            1147..>2501
                     /locus_tag="VF870_16250"
     CDS             1147..>2501
                     /locus_tag="VF870_16250"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559815.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX9253800.1"
                     /translation="MQINRFFTEQGKDPLSSFEFSKRTSEIKNPDGSSVFRMENVSVP
                     KQWSQVATDIIAQKYFRKAGIPKLLKKVNEKGVPKWLQPSVADAERLQELPENDRFTS
                     ETDSKQVFHRLAGTWTYWGWKANYFDSEDDAKNFYDELMYMFANQMAAPNSPQWFNTG
                     LNWAYGITGPSQGHFYVDYQTGELTKSDDAYTHPQPHACFIQSVSDDLVNEGGIMDLW
                     VREARLFKYGSGTGSNFSELRGAHESLSGGGKSSGLMSFLKIGDRSAGAIKSGGTTRR
                     AAKMVTLDLDHPDIEEYINWKVIEEQKVAAIVAGSKLCNLHLNNIMKACYDEHPENDR
                     FNKKLNKKLSYAVLEARKAQISNNYIERVIHLAKLGFKSIEFPIYDTDWNSEAYATVS
                     GQNSNNSVRVTNEFMTAVLTDGNWNLYWRTEKRKAKKEKRTPKPCKTLKARDLWDQIA
                     YS"
BASE COUNT          855 a          404 c          419 g          823 t
ORIGIN      
        1 tgttgttttt tcttcatcat ttacttcaaa agacacttta agccttttag cgttaagtaa
       61 atccttaaca taaccttcga cttttgtggt ccctttattg ataataatca ttctatttgc
      121 aacaagttca acctcataaa gtatgtgtga agaaagaaaa attgtcttct tttcaacctt
      181 gctgagatgt aaaatcagat cccggatttc cttcattcct tgcggatcta aacctgtagt
      241 aggttcatca agaattatca aatcgggatt gtgaagtaat gcttgcccta atcccaatct
      301 ctgtttcatt ccgtgtgaaa aagttttaac tttactatta tcacgtttgc tcaaaccaac
      361 aagttcaagc acttccataa ttctgttctt acttatttct ttacctgaaa ttcttccaag
      421 aatttcaaga tttttataag ctgataaata accgtagaaa tcgggttttt ctacaatcgc
      481 accaattctt ttaagaatct ctattcgatt tttttcaaga gttttattaa ataattttat
      541 gcttccttcg gttggtttaa taagcgataa aagcattctt atagttgtgc ttttacccgc
      601 gccatttggg cctaaaaacc caaatacatc accttcaaaa acattaagat taagactatt
      661 tacagcacgc aaatctttaa agtctttggt taatgagtta atctcgatta tcttgtcgcc
      721 gttcaatata aatctttcaa aaaattgatt aaaatcatga taaagatagt aactaatcaa
      781 gaattaaaag atatatttat tttgagcggt aattattttt gatgaagaag ttgtgatttt
      841 cgaatgatgc taaaattaat ttattgtcaa gagtaagttg tataaaattt aatttggttt
      901 ttaaaaaaat tattttcatt ttcgatttga aaattaactg ataaataaaa aataaaatta
      961 ttctactttc agttttttat tgataatttt ttttaatagt ttctgatgtg aattgctgat
     1021 gagtgatgta aaaaaaatat taagtttttt taattgcaaa acatcattca aaaattccgg
     1081 atcacttcta accttaatta acataaatag tttttttatt aactagtcta tcctctggag
     1141 agttggatgc agattaatcg attttttacc gagcagggaa aagatcctct atcatctttt
     1201 gaattttcta aacgaacctc agaaattaaa aatcctgatg gttcaagtgt atttagaatg
     1261 gaaaacgttt cggtgccaaa acagtggtct caagttgcca cagatattat tgctcagaag
     1321 tattttagaa aagccggtat tccaaaactt cttaaaaaag taaatgaaaa aggagttcca
     1381 aaatggcttc agccttctgt tgctgatgcg gaacgattac aggaacttcc agaaaatgat
     1441 agatttactt cagagacaga ttccaaacaa gtgtttcata gattagccgg aacatggact
     1501 tattggggtt ggaaagccaa ttactttgat tctgaagacg acgcaaaaaa cttttatgat
     1561 gaattaatgt acatgtttgc caatcaaatg gcagcaccaa atagtccgca atggtttaac
     1621 accggattaa actgggctta tggaataacc ggaccttctc agggtcattt ttatgttgat
     1681 tatcaaaccg gagaattaac aaaatccgat gatgcatata ctcatcctca gccgcatgct
     1741 tgttttattc aatcagtaag tgatgacctg gttaacgaag gcggtataat ggatctttgg
     1801 gtccgtgaag caagattatt taaatatggt tccggaacag gatcaaactt ttctgaactt
     1861 agaggcgctc atgaatcttt aagcggcggt ggaaaatctt ccggtttaat gtctttctta
     1921 aaaatcggtg atagatctgc aggagcgatt aaatccggcg gaacaaccag acgcgcagct
     1981 aaaatggtaa ctcttgatct ggaccatccc gatattgaag aatatataaa ttggaaagtt
     2041 attgaagagc aaaaagttgc tgcaattgtg gcaggctcaa agttatgtaa tcttcatctt
     2101 aacaacatta tgaaagcttg ttatgatgaa catcctgaaa atgatagatt caataaaaaa
     2161 cttaacaaga aattaagcta cgcagtttta gaagccagga aggcacaaat atctaataat
     2221 tatatcgaac gggttataca tcttgcaaaa ttaggattta aatctatcga gttccctatt
     2281 tatgacactg attggaattc agaggcctat gcaactgtat caggacaaaa ttcaaacaat
     2341 tcagtgcgcg ttacaaacga atttatgacc gcagtactta cagatggaaa ttggaatcta
     2401 tattggcgaa ctgaaaaaag aaaagcgaag aaagaaaaaa gaactccaaa accttgtaaa
     2461 actttaaaag caagagattt atgggatcaa attgcatatt c
//
LOCUS       DASWBY010000902         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_334811, whole genome shotgun sequence.
ACCESSION   DASWBY010000902 DASWBY010000000
VERSION     DASWBY010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334811"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..728
                     /locus_tag="VF870_16255"
     CDS             321..728
                     /locus_tag="VF870_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253801.1"
                     /translation="MKNKIVIIDQFIELRALGYTFDEIVKELGVSKVTLLRWNKKHND
                     EIDATKKEMVRSAAEKIALKNAYMISQFADLVYRDNRKEKKRQVIDEADIKRFYKRVF
                     QVYAREMKGLSVNFDKDGKPKNVVIEWKDGKSD"
     gene            complement(1090..2973)
                     /locus_tag="VF870_16260"
     CDS             complement(1090..2973)
                     /locus_tag="VF870_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253802.1"
                     /translation="MKSEIVLVLILIFNSVYILAQNALPTGTSVLFNGSGNCILCHQG
                     NGSAMTWNGQDVSPITYWRSTMMGNASKDPLWRAMVAEEVNNFPQHQQMIETTCTKCH
                     SPMGFTQAIYNGQSGYSMAELKQDPLANDGVSCTVCHQIKPDNFGTPQSYSGNYIIHA
                     DSILYGPYENSDTTLMRAIIGYKARYSSHINQSELCATCHTLFTPTLNEQGNVVGSFA
                     EQTPYLEWKNSVYPSQNVQCQDCHMPKIYDPIKISGMGSFPNRTPFWRHTFVGGNVYM
                     LNLLKNNIDSLGLTAEPNHFDSTISRTEFNLMNQAVELTVSSGYQNEMLQVKVFIKNL
                     TGHKIPTGIPFRRIWIHLKVQDQNSNTIFESGGWDNTGKIINYNSDYEPHYDLITSED
                     QVQVYEGVFTDVNNQVTHTLLKAAQYVKDNRLPPIGFTTTHPSYDSIKIVGNANDDTN
                     FNRYGTYQGGTGGDSVTYLVPALADNNYNITVEVCYQSVKTQLIDHIRNIDLVDINRF
                     VSMYDALPNFPFIMKREVLDIVSGVDDESNLVNSFSLEQNYPNPFNPSTIIKYRVPSI
                     SHVSLKVYDVLGNEVATLVNEEKPAGSYELNFDAPQLSSGIYFYKLQSESFVETKKML
                     LLK"
     gene            complement(3004..3777)
                     /locus_tag="VF870_16265"
     CDS             complement(3004..3777)
                     /locus_tag="VF870_16265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253803.1"
                     /translation="MKNIFTIKIIFCFLISLTLFRGGFSQSSIEFQTGTTIEVTSGAD
                     ICAETITINGTYSGDGTKCNGPLPVELSLFTATISGGDVRLNWKTETEINNFGFEIER
                     QNGLLESSNSKFEKIGFVNGNGNTNSPKEYSFRDQRPTGGNKFLYRLKQIDNDGQFEF
                     SDIVEVEIVPVKFELSQNYPNPFNPSTTIRFSLPVQTHLKINIYNMLGELVKTISEGT
                     YEAGYYSVSFDAAELSSGTYIYRIESENFTETKKMILMK"
     gene            complement(3779..>4744)
                     /locus_tag="VF870_16270"
     CDS             complement(3779..>4744)
                     /locus_tag="VF870_16270"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEX9253804.1"
                     /translation="LGYQSLYLNLIGSQNTAIGSNSLRSNTGSSNTAVGYSALYTNSN
                     GTQNTAVGYNSLLVNSTGSYNTALGYNSLGSNSTSSYNVAVGFNSLANNSTGTQNTSI
                     GGYAGSTVSTGSNLTLIGYNSSPSIANATNEVTLGNNQVTTLRCNVQTISSLSDLRDK
                     KNIKDLSLGLDFIMKVKPREFNWDRRDWYENGVPDGSKMSEQPTAGFIAQEFDSLQTS
                     EHAEWLTLVLKNNPDKWEATYGNLLPVIVKAIQDLKKEKDELQNKYEKLLSFNQKLAI
                     NNELLTKEINSIKTSISGEIEFQVKSILSKTQKQENGNNKLTLGN"
BASE COUNT         1546 a          802 c          846 g         1550 t
ORIGIN      
        1 gatattgcgg cttaaatgtc attctttatg agataaattc gataaaaagg taggcaaaag
       61 tgggcaaaaa agtcagcaat agtcagcaat agtcagcaaa aatatttaat ttgtgatgaa
      121 aagcgctttt tttgcttaaa attgaatgct tttggagcag aggttattat aaaagtttac
      181 tatatgtata ctaaacaaca aatttataga cgtaatttct aataatttta tcagttttaa
      241 taaaaaaatg ataccaaaag ttcactaaaa gctaaatatg atataaagta ttttcattca
      301 ataattacga cggaggatta gtgaaaaata aaatagtgat catagatcaa tttatagagt
      361 taagggcact tggatatact tttgatgaaa ttgtaaagga gctcggagta agcaaagtaa
      421 ctttactaag atggaataaa aaacataatg atgaaatcga tgcaactaaa aaagagatgg
      481 tacgatcggc tgcggaaaag atagctctaa agaatgcata tatgatatcg caatttgcag
      541 atttggttta cagggataat cgaaaagaga aaaagagaca agtaatagat gaagctgaca
      601 taaaaagatt ttacaagcgt gtttttcagg tgtatgccag ggaaatgaaa ggtttatcag
      661 taaattttga taaagatggg aaaccgaaaa atgtagtgat agaatggaaa gatggaaaga
      721 gtgattagtg aacaatatac gtgagatgag aaacgaaagt cgtgagatga ggtgattagt
      781 aaatagtgag tagtgaatcg cggatcttgc aggcaggtgc tggatactgg atgctggata
      841 ctggaataaa aattaagagt taagaaataa gaattaagaa ataaaaatat aggggaaaaa
      901 ggccgattag aatctgcgca agtagtaata aaaaaaggga tttacagtgg acaggacacc
      961 ataaatccct tggtgctccg caaactcaaa tctcttagaa ctttcttttc cggcaataaa
     1021 gagctattta cctatcaata ctgtgttaaa atgttggctg ggaggtaatt ttcccagctt
     1081 tccatttgct tacttcagca gcaacatctt tttagtctca acaaaacttt cagattgtaa
     1141 tttgtaaaaa taaattcctg atgataattg cggtgcatca aaatttaact cataacttcc
     1201 ggcaggtttt tcttcattaa caagagtagc cacttcattc ccaagcacat cataaacttt
     1261 aagggacacg tgcgagatgc tgggtactcg atacttgatt atagtagaag ggttaaatgg
     1321 attaggataa ttctgctcaa gagaaaaact gtttactaaa ttactttcat catcaacacc
     1381 ggaaacgata tctaatactt ctctcttcat tataaatgga aaattcggca gagcatcgta
     1441 catactcaca aatctgttaa tatcaacaag atctatgttt cttatgtgat cgatcaattg
     1501 agtttttacc gattggtaac aaacctcaac tgtaatgtta taattattgt ctgcaagagc
     1561 cgggactaaa taagtaacac tatctccacc tgtacctccc tgatatgtac cgtatctgtt
     1621 aaagttagta tcatcatttg catttcctac aatttttata gaatcataac ttgggtgagt
     1681 cgtagtaaat ccgattggtg gaaggcgatt atcttttaca tactgagcag cttttagaag
     1741 ggtatgtgta acttgattat taacatctgt gaaaacgcct tcataaacct gaacttgatc
     1801 ttcagatgta atcaaatcat agtgaggttc ataatcagaa ttgtagttaa taatctttcc
     1861 tgtattatcc catccaccgg actcaaagat tgtgttacta ttctgatctt gaactttcaa
     1921 atgaatccag attcttctga aagggatacc agttggaatt ttatgaccgg ttaaattttt
     1981 tataaagacc tttacttgca gcatttcatt ttgataaccc gatgaaacag taagttcaac
     2041 cgcctgattc ataagattaa attcagtcct tgaaatagta gaatcaaaat gatttggctc
     2101 ggctgttaat ccaagtgaat caatattatt ctttagcagg ttaagcatat agacattacc
     2161 accaacaaaa gtatgccgcc agaatggtgt cctgttagga aagcttccca ttcctgaaat
     2221 cttaatcgga tcataaatct taggcatatg acaatcctga cattgaacat tttgcgaagg
     2281 atatacacta ttcttccatt caagatatgg ggtttgttct gcaaaacttc caactacatt
     2341 accctgctca ttaagggttg gtgtaaatag agtgtggcag gtcgcgcaaa gttcagattg
     2401 atttatatgt gaactgtacc gggctttata accgataatt gctctcatta gtgttgtgtc
     2461 agaattttca taaggtccgt ataaaatact gtcggcatga ataatataat ttccggagta
     2521 cgattgcgga gttccaaaat tgtcaggttt tatttgatga catacagtac agctaacccc
     2581 atcatttgct aatggatcct gtttcaattc agccatcgaa taacctgatt gtccattata
     2641 aattgcctga gtaaatccca ttggcgaatg acattttgta caggttgttt ctatcatttg
     2701 ctgatgttga ggaaagttgt ttacttcttc tgcaaccatt gcccgccata gaggatcttt
     2761 gcttgcattg cccatcatag ttgaacgcca ataagtaata ggtgaaacat cctgaccatt
     2821 ccaggtcatt gcacttccat tgccttgatg acaaagaatg caattgccag aaccattgaa
     2881 caaaacagag gttcctgttg gcaaagcatt ttgtgccaat atataaactg aattaaaaat
     2941 aagaattaaa actaaaacta tttcactttt cattaacgta atccggaatt tttttaagac
     3001 aagttatttc atcaggatca ttttcttagt ttctgtaaaa ttttcacttt ctattctgta
     3061 gatatatgta ccgcttgata attccgcggc atcaaaagaa acgctgtaat atcccgcttc
     3121 atacgtccct tctgaaatgg tttttactaa ctcaccaagc atattgtaaa tatttatctt
     3181 taaatgtgtc tgaacaggta aagaaaaccg tatagttgtg ctggggttga acggattggg
     3241 atagttctgg cttaattcaa atttaacggg aactatttca acttcaacaa tatccgaaaa
     3301 ttcgaactgt ccatcattat caatttgctt tagtctgtat aaaaatttat ttccacccgt
     3361 gggcctttga tctctaaaag aatattcttt cggtgaattt gtattaccat taccatttac
     3421 aaatccaatt ttttcaaatt tgctgtttga agattcaagt aagccatttt gtctttcaat
     3481 ttcaaatcca aaattattta tctctgtttc tgttttccag tttagcctaa catcaccccc
     3541 agaaatcgtt gctgtaaata aagagagttc aacaggaagc ggaccattgc acttcgttcc
     3601 atctccagag taagttccat taatagtaat agtttcggca caaatatctg ctccgctagt
     3661 aacctcaata gtagtcccgg tttgaaactc tattgaactt tgactaaatc caccacgaaa
     3721 caaagtcagt gagattagga aacaaaaaat aattttaata gtaaagatat ttttcatatc
     3781 agtttcccaa tgtcagttta ttatttccat tttcttgctt ttgagttttt gataaaatac
     3841 tttttacctg gaattctatc tctccagaaa ttgaagtttt tattgaattt atttctttgg
     3901 ttagcaattc attgttaatt gccaatttct gattaaagga aagtagtttc tcatatttat
     3961 tctgaagctc atctttctct tttttcaaat cctgaatagc ttttacaatt acgggtaata
     4021 aatttccgta cgttgcttcc catttatctg gattgttttt taacacaagg gttagccatt
     4081 ctgcgtgctc tgatgtttga agtgaatcaa attcctgggc aataaaacct gctgttggtt
     4141 gttcactcat tttgcttcca tctggaactc cgttttcata ccaatctctt ctgtcccaat
     4201 taaattctct tggtttcact ttcataataa aatccaatcc gagagacagg tctttgatat
     4261 tctttttatc tcttaaatct gaaagtgaag atattgtctg aacattgcaa cgaagtgtag
     4321 taacctgatt attaccaagt gtgacctcat tcgttgcatt cgcgattgaa ggggatgagt
     4381 tatatccaat gagtgtaaga ttactcccgg tagaaacggt gcttccagca taacctccaa
     4441 tagatgtgtt ctgtgttcca gtggaattgt ttgcaagaga gttaaagcca accgcaacat
     4501 tgtatgaact tgtagaatta gatcccagag aattataacc caatgcagtg ttataacttc
     4561 ctgttgaatt aactaacaac gaattataac cgaccgctgt gttttgtgtg ccatttgaat
     4621 ttgtgtataa agctgaatag ccgactgcgg tattagaaga accagtattc gaacgcagag
     4681 aattgctacc gattgcagtg ttttgtgaac caattaaatt caagtacaaa gattggtatc
     4741 caag
//
LOCUS       DASWBY010000903         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_450844, whole genome shotgun sequence.
ACCESSION   DASWBY010000903 DASWBY010000000
VERSION     DASWBY010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450844"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..81)
                     /locus_tag="VF870_16275"
     CDS             complement(<1..81)
                     /locus_tag="VF870_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEX9253805.1"
                     /translation="MHWIILFIAGLFEVAWAIGLKYTDGFS"
     gene            complement(83..2200)
                     /locus_tag="VF870_16280"
     CDS             complement(83..2200)
                     /locus_tag="VF870_16280"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX9253806.1"
                     /translation="MKNQFSCRSLFFALVFIANFAFAQTYYGINPDTVKAQKFDMGKM
                     WTFENPPLDYFEKEYGFRPSQELLEKFQKSALKFGGGCSASFVSEDGLIMTNHHCVRG
                     ILSTVQKDDENILRDGFYAKTLEEERTIPNLKVEQLIIVKDVTKEIQSAMDKVKTDAE
                     KIEARDKKIEELKKINSDQHPELTFKITSLYNGGKYSLYGYKVYSDIRLVFVPELFVA
                     KLGGDPDNFTYPRYGLDCAFLRAYEDGKPIKTDYFFSWNNDGVVEDQPVFVVGNPGRT
                     NRINTMVQIEYARDIQYPMLVGMFKEMYSIYEEMVNETNAEDFKLIARLYTIGNALKV
                     YSGTYKALLDPFLVARKKDFEKNFKESVDKNPVLKEKFGHIWSELETSRNQARKDAHK
                     IYAYTISNFYSPQYLIMAKSLVNYANQLKDNEITKEKYDSLVTQLFPADFNKELQDKL
                     LLVQLNILKNNLTSEDEIVKQLIGNNPTDQAADNIISKSSLTSKEKFLDLAEGGYEAV
                     LNSKDPFIYYVLNTQDELKKMQDENQARVDREEILNQALGEALYKVYGDAIPPDATGT
                     LRLADGIVKGYDYNGTRAPIKTTFYGALDRYYSFDKKFPFNLPKYFENLPKEFDPSTP
                     LNFVSTNDIVGGNSGSAVLNKNAEIVGLAFDGNIESLPSNFIYTTEANRTVCVSALGM
                     IEAIRDLYKATRLNDEITNGKLR"
     gene            2479..2814
                     /locus_tag="VF870_16285"
     CDS             2479..2814
                     /locus_tag="VF870_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEX9253807.1"
                     /translation="MNIQIIGTKKCKETQKAERYFKERRIPFHYRDLTEKGLAKGELN
                     NISRVIPLDDLIDRESKRYKDRGMQFMVFDVEEELLTDPLLLKTPIVRNERFVTVGYE
                     PNVWKEWIK"
BASE COUNT          944 a          522 c          466 g         1069 t
ORIGIN      
        1 tgaaaagcca tctgtatatt ttaatccgat agcccaggcg acttcaaaca atccggcaat
       61 aaataaaatt atccaatgca tattatctta gctttccgtt tgttatttca tcgtttaatc
      121 ttgtggcctt gtaaagatca cgaattgctt cgatcattcc aagtgcactt acgcatacag
      181 ttctgtttgc ttctgttgta tatataaaat tacttggtaa actttctatg tttccatcaa
      241 aagctaatcc aacaatctct gcatttttat ttaacactgc actacctgag ttaccgccaa
      301 caatatcatt tgtgctgaca aagtttaaag gagttgatgg atcaaactct tttggtaaat
      361 tttcaaaata cttaggaaga ttaaagggga attttttatc gaaagaataa tagcgatcta
      421 atgctccgta aaatgttgtt tttattggtg cccgggttcc attgtaatca taccccttta
      481 caataccatc tgccaaacgt aaagtgcctg tcgcatcggg tggaatagca tcaccataca
      541 ccttataaag tgcttcacct aaggcttgat ttaatatttc ttccctatct actctggcct
      601 gattttcatc ctgcattttt ttcagttcat cttgagtatt aagcacatag taaataaaag
      661 gatctttaga atttaatact gcttcgtatc cgccttcagc taaatctaaa aacttttctt
      721 tgcttgtaag actggatttt gaaattatat tatctgctgc ctgatctgtt ggattattgc
      781 ctatcaattg tttaactatt tcgtcttccg aagttaaatt attcttaagg atgtttaact
      841 gaacgagaag taatttatcc tgtaattctt tattaaaatc cgctggaaat aattgagtta
      901 caagtgaatc atatttttct tttgtgattt cattatcctt taactgattt gcataattta
      961 caagactttt agccattata agatattgag gtgaataaaa gttacttatc gtgtaagcat
     1021 agattttatg tgcatctttt cttgcctgat tgcgagatgt ttcaagctcg ctccagatat
     1081 gtccaaactt ttcttttagc acgggatttt tgtctactga ttccttgaaa ttcttttcaa
     1141 aatctttttt acgggcaact aaaaaaggat caagaagcgc tttgtatgtg ccggaataaa
     1201 ctttaagagc atttcctatt gtgtacaatc ttgcaattaa tttaaaatct tcagcatttg
     1261 tttcattaac catttcctca tagatactgt acatttcttt aaacattcca acaagcattg
     1321 gatattgaat gtctctagca tattcaattt gtaccatagt gtttattcta tttgttctgc
     1381 ccggatttcc aacaacaaac accggttgat cttcaactac accatcatta ttccagctaa
     1441 agaaataatc tgtcttaatt ggcttaccat cttcatatgc acgaagaaaa gcacaatcca
     1501 gtccgtaacg cgggtaagta aaattatcgg gatcaccacc aagcttcgct acaaataatt
     1561 ccggtacaaa aactaaacgt atatcgctat aaactttata gccgtacaaa gaatatttgc
     1621 cgccgttgta tagtgaagta attttaaaag ttaattcagg atgttgatct gagtttattt
     1681 tttttaactc ttcaattttc ttatcccggg cttctatttt ttcagcatca gtttttactt
     1741 tatccattgc tgattgaatt tcttttgtta catccttaac gattatcagc tgttcaactt
     1801 taagatttgg tattgttcgc tcttcctcta aagtttttgc ataaaatcca tcacgtaaaa
     1861 tgttttcatc atctttttga acagtggaaa gtattccacg tacacaatgg tgattagtca
     1921 ttattaatcc atcttccgaa acaaatgaag cagaacatcc gccgccaaat tttaaggcgc
     1981 ttttttgaaa tttctctaac aactcctgtg atggtctaaa tccatattct ttttcaaaat
     2041 aatcaagtgg agggttttcg aacgtccaca tttttcccat atcaaatttt tgcgctttaa
     2101 ctgtatcggg atttatacca taataagtct gcgcaaatgc aaaatttgca ataaaaacta
     2161 gtgcaaaaaa taaactgcgg cagctgaact gatttttcat tttacatttc caatttgatt
     2221 ttatattcat cgcaaattaa aaatttaaga tgggaattgg aaaccatttt ggtggattca
     2281 agcaaaatcc gtatcaaata ctttagtctg tgtttgagat tctttacgtt ttatttacaa
     2341 ctaaatttta gtttcgttat tatttcatta tataaaacaa taagcagact aaagtctact
     2401 ctacgttaat ataaaaatga gttttatatt tcactacaga attttgtcat ccttgttata
     2461 ttaagtaaag gattaaatat gaacatccag attatcggaa caaagaagtg taaagaaaca
     2521 caaaaagctg aacgctactt taaagaaaga agaattccat ttcactatag agatttgact
     2581 gaaaaaggat tagcaaaagg cgagcttaat aatatttcaa gagtaattcc tttagatgat
     2641 ttaatagata gagaaagtaa aagatataaa gatcgtggta tgcagtttat ggtttttgat
     2701 gttgaagaag aattgttaac agatcctttg cttcttaaaa ctccaattgt aagaaatgag
     2761 agatttgtta ctgttggata tgaacctaat gtttggaaag agtggataaa gtgaaagtca
     2821 ttgcgaacga ggctgcgagt gaagcaatct gcatattgca aaaacagatt gcttcgttgc
     2881 actcgcaatg acaagcaaga actaattaaa catcgtaata caaaccgaac tcgtatgggt
     2941 gcggctgaag tgccattggt ttcacttctt tatccatttt atattttatc caggtttcaa
     3001 t
//
LOCUS       DASWBY010000904         4862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_632303, whole genome shotgun sequence.
ACCESSION   DASWBY010000904 DASWBY010000000
VERSION     DASWBY010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632303"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF870_16290"
     CDS             complement(<1..256)
                     /locus_tag="VF870_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX9253808.1"
                     /translation="MFDKQVYIDRRIELKKNFKTGVILFLGNDESAMNYAGNTYAFRQ
                     DSSFLYYFGLDVPNLVGIIDIDENKEILFGYEFNLEDIVWM"
     gene            complement(408..1220)
                     /gene="lgt"
                     /locus_tag="VF870_16295"
     CDS             complement(408..1220)
                     /gene="lgt"
                     /locus_tag="VF870_16295"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX9253809.1"
                     /translation="MIISSIFWNVSPEIVKLGPISLRWYGLLFASGFVLGYLILTKIY
                     KLEKKPQADLEQLSIYVILGTVIGARLGHCLFYDPVYYLSNPLEIIKVWQGGLASHGA
                     AIGILTSLYLLTKKQKDKTMLWILDRVVIVVALAAVLIRLGNLFNSEIIGKPTDLPWA
                     FIFARVDNVPRHPAQLYESIFYLISFIILYFVYYKTEKKSKQGFIFGLFLVLIFGFRF
                     FVEFVKMEQSAFEKGMILDMGQLLSIPFVLGGLYFIFRKEKAIANKNLKTKT"
     gene            complement(1457..3802)
                     /gene="topA"
                     /locus_tag="VF870_16300"
     CDS             complement(1457..3802)
                     /gene="topA"
                     /locus_tag="VF870_16300"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX9253810.1"
                     /translation="MAKNLVIVESPAKAKTIEGYLGKDFLVTSSYGHIRDLEKKNKGV
                     EIENEFKPVYKISEDKKKVVTELKKLSKKAETVWLATDEDREGEAISWHLKEALDLEE
                     DKIKRIVFDEITKTAILNAIKNPRKIDYHLVDAQQARRILDRLVGFEISGILWRKIKS
                     KLSAGRVQSVAVRLIVEREREIDAFVPESRFKVVANFTVTDEKGNKTNLRAELARYLK
                     NEKEVEEFLKSCSTSDFIVDDVEKKPVKRSPAAPFTTSTLQQEAARKLRYSVSRTMLL
                     AQRLYESGKITYMRTDSVNLSDFALDAAEQAIKNDFGKQYFEKRQFKTKSKSAQEAHE
                     AIRPTDFNKENVDGPKDEQALYELIWKRAIASQMSDAEIERTTAKIKITGNKDVLTAK
                     GEVIKFDGFLKVYLEDTDDENLENGDDAILPPLKVGQKLDMNEIIATERYTRPSARFT
                     EASLVKKLEELGIGRPSTYAPTISTIQKRGYVHKEERMGIERKFRVLSLNKKKEINRE
                     TKTEITGADKGKLFPADIAMIVTDFLNQHFPQIMDYQFTALVEDELDEIALGKLSYSK
                     MLKEFYFPFHEKVEHTTETSERASGERILGVDPKTKKTVSVRMARFGPVAQLTDPNDP
                     EAKPEYAGIRRDQKLELITLEEALELFKLPRVVGTFEDKDIVAAIGRFGPYLRHDGKF
                     YSLKKEYDPHTVSEENAIIVIEAKRVADAEKFIKVFDEDPTFQILNGRWGPYLKAGKK
                     NIKLPKDRDPASFTYEECVELDKNALESKGRKRTFRKKKEE"
     gene            4149..4394
                     /gene="yidD"
                     /locus_tag="VF870_16305"
     CDS             4149..4394
                     /gene="yidD"
                     /locus_tag="VF870_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX9253811.1"
                     /translation="MIKKYIDPFLKILAVPFIVLIKVYQLLISPLFPSSCRYTPTCSH
                     YTAEALKKYGIIKGGWLGIKRISRCHPWGGSGYDPVP"
     gene            4405..>4862
                     /locus_tag="VF870_16310"
     CDS             4405..>4862
                     /locus_tag="VF870_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9253812.1"
                     /translation="MAKKPSSKKISFRKRIFNTFKWILKFAVIFFITTVLLVFAMRWI
                     NPVTSSIMVQRQISGLFSGEFELVNYHWVNYDDVSKFMPIAIVAAEDQNFPNHFGFDF
                     KQIEKALKENKRGRRVRGASTITQQVAKNLFLWEGKSFVRKGIEAYFTLL"
BASE COUNT         1528 a          853 c          763 g         1718 t
ORIGIN      
        1 ccatccagac aatatcctca agattaaact catatccgaa aagaatttct ttattttcat
       61 cgatatcaat tatcccaacc agatttggta catcaagtcc aaagtaataa agaaatgagc
      121 tatcctgtct gaaggcatat gtgttacctg catagttcat tgcggattca tcattcccaa
      181 ggaataagat aacgccggtt ttaaaatttt tctttaattc aatcctgcga tctatgtaaa
      241 cttgtttatc aaacataaat gctccttaaa agaatatatg taattatatc tgatgttaaa
      301 ttatggaata aaagaatgga tttccataag agaagaaaaa tggtatgatg attataacaa
      361 ttttattaag atccaaaggg atcagggtat attattaata attgtcgtta tgtcttagtc
      421 tttagatttt tattggcaat cgctttttct tttctaaata taaaatacaa tccgcctaat
      481 acgaatggta tacttagaag ttggcccata tccagaatca tccctttttc aaaggcagat
      541 tgttccatct ttacaaactc cacaaaaaat ctgaaaccaa aaatcagtac aagaaacaat
      601 ccaaagataa atccttgctt agattttttt tcagttttgt aataaacaaa atataaaatg
      661 ataaaagata tcaggtaaaa aattgattca taaagttgtg ccggatgtct tggtacatta
      721 tcaactcttg caaaaataaa tgcccatgga agatcagtgg gtttaccaat aatttcggaa
      781 ttaaataaat ttcctaatcg aataagaact gcagcaagag caactacaat taccacgcgg
      841 tcaagaatcc ataacattgt tttatctttt tgcttttttg taagaagata caatgaagtt
      901 agaattccaa ttgcagcacc gtggcttgcg agtccgccct gccaaacctt aataatctct
      961 aatggatttg ataaataata aacaggatca taaaataaac aatgacccaa acgtgcaccg
     1021 ataacggttc caagtataac atagattgaa agttgttcaa gatctgcttg tggtttcttt
     1081 tcgagcttgt aaatttttgt aagtatcaaa tatcctaaaa caaatccgga tgcgaaaagt
     1141 aatccatacc agcgaagtga aattggtcca agctttacta tttcagggct gacattccaa
     1201 aatattgacg agatgatcat tatagtctgt aaaattgttt atgatttaat tgtaaatata
     1261 ctttgatgat tttagtgatg caaagaataa aagaatcata ctaaagaagg tcatttcgac
     1321 gactataagg aggagaaatc tgtaacctaa tattttcttt gaaatctgtt tttgttgaaa
     1381 tcagatttct tagtcgaaga ctccttcgaa atgactatta aaaagatcct gggtcaagcc
     1441 cgggatgaca taattattat tcttctttct tttttctaaa agttcttttc ctgcctttgg
     1501 attctaaagc atttttatca agctctacac attcctcata agtaaaagat gcaggatcgc
     1561 gatccttagg aagcttaata tttttctttc ccgctttgag atatggtccc catcttccgt
     1621 taagaatctg gaatgtcgga tcctcatcaa aaacttttat aaatttttca gcatcggcta
     1681 cgcgctttgc ctcaataact ataattgcat tttcttcaga aacagtgtgt gggtcatatt
     1741 cttttttcag tgaataaaat tttccgtcgt gtcttaagta aggtccaaat cttcctattg
     1801 ccgcaacgat atctttatct tcaaatgttc caacaactct gggaagttta aataactcca
     1861 gcgcttcttc aagagtaata agttctaatt tctgatctct tctaatgcca gcatattccg
     1921 gttttgcttc aggatcattt ggatcagtca attgtgcaac tggaccaaag cgtgccatac
     1981 gaaccgaaac agtttttttt gttttaggat caaccccaag aattctttca ccggatgcac
     2041 gttcacttgt ttcagttgtg tgttcaactt tttcgtgaaa tgggaaataa aattctttaa
     2101 gcattttact gtagcttaat tttcctagtg caatttcatc aagctcatct tcaacaaggg
     2161 ctgtaaactg ataatccatt atctgcggaa aatgttggtt aagaaaatca gtaacaatca
     2221 ttgcaatatc agcagggaaa agtttgcctt tatccgcacc ggtaatttct gttttagttt
     2281 ccctattaat ttcctttttc ttatttaaac ttaaaactct aaacttgcgt tcaattccca
     2341 ttctttcttc tttgtggaca taaccgcgct tctgaattgt gctgatggtt ggtgcgtatg
     2401 ttgatggtct tccaattcca agctcttcta atttcttaac aagacttgct tcggtaaatc
     2461 ttgcagaagg acgagtatat ctttcagttg caataatttc attcatatca agtttctgcc
     2521 cgacttttaa cggagggaga attgcatcat caccattttc taaattttca tcatcagtat
     2581 cttcaagata aacttttaag aaaccgtcaa actttatcac ttcgcctttt gctgttaaaa
     2641 catctttgtt gcccgtgatt ttaatttttg cagttgttct ttcaatttct gcatcactca
     2701 tttgggatgc tattgcacgt ttccagatca attcatacaa agcctgttcg tcttttggtc
     2761 catcaacatt ttctttatta aaatctgtcg gacgaattgc ttcgtgtgct tcctgagcag
     2821 atttagattt tgttttaaac tgtctttttt caaaatattg tttcccaaaa tcatttttaa
     2881 ttgcttgctc tgcggcatca agagcaaagt cagaaagatt tacagagtca gtacgcatat
     2941 aagtaatctt accggattca taaagtcttt gtgcaagcag catagttctg gaaactgagt
     3001 atcgtaattt gcgtgctgct tcctgctgca gagtagaagt tgtaaatggt gcggctggtg
     3061 aacgttttac aggcttcttt tcaacatcat caacaataaa atctgatgtt gaacagcttt
     3121 ttaaaaattc ttcaacttct ttttcgtttt ttaaatatcg tgcaagttct gccctaagat
     3181 tagttttatt tcctttttca tctgttactg taaagtttgc aacaacttta aatcgtgatt
     3241 ctggaacaaa agcatcaatc tctcgctcgc gctcaacaat taatctaacg gcaacagatt
     3301 gaactcttcc tgcggagagt tttgatttaa tttttctcca taaaattccc gagatttcaa
     3361 atccaaccag gcgatccaaa attcttcttg cctgctgtgc atcaacaaga tggtaatcaa
     3421 ttttccttgg attttttata gcattaagaa ttgccgtctt tgtaatttca tcaaaaacaa
     3481 ttcttttgat cttatcttct tcaagatcta atgcttcttt aagatgccat gaaattgctt
     3541 caccttcacg gtcctcgtca gttgcaagcc aaacagtctc ggccttttta gaaagttttt
     3601 ttaattcggt aacgactttc tttttatctt cagaaatttt ataaaccggt ttaaattcat
     3661 tttctatctc aacgcctttg tttttctttt caaggtcacg gatgtgtccg tagcttgatg
     3721 tgacaagaaa atctttacct aaataacctt caattgtttt tgctttagca ggagattcta
     3781 caataactaa gttttttgcc attatgtttt cttttattca tcttatgcaa ttttttataa
     3841 aataagatga ttaaattatt tcactctttc acttcgctta gagtgactaa tttgtttgat
     3901 tatttatata gtcatactta gcgaagtaga agtatgacat atgctattca tattttaaat
     3961 taagaaatat tataattcag tataaataaa ttctttaact ttaaaaaaac gcttgcaaat
     4021 taaccgataa ataaaacaaa attcaaagtt atttggtaag cctcatcatt tatttggcaa
     4081 ttagtccgtt tttatgatga aaaatttgat aatatttgat gtgcaaatca caattattga
     4141 ttgaattgat aataaaaaaa tatatagacc cgtttttaaa aattttagca gttccattta
     4201 tcgtactcat taaagtttat caactgctga tttctccgtt atttccatca tcctgcagat
     4261 atacaccaac ttgttcgcat tacacagcag aggcattaaa aaagtatgga ataattaaag
     4321 gcggttggtt gggaataaaa agaatttcac gctgtcatcc ctggggaggc agtggttatg
     4381 atcctgtacc gtaaataaaa ttctatggca aaaaaaccat catcaaaaaa aatatctttc
     4441 agaaaaagaa tctttaacac ctttaaatgg attttaaagt ttgcagtaat tttctttatt
     4501 acaacagtgc ttcttgtttt tgcaatgaga tggattaatc cggttactag ttcaattatg
     4561 gttcaaagac agatttccgg attgttctct ggagaatttg agttagtaaa ttatcactgg
     4621 gttaattacg atgatgtttc taagtttatg ccgattgcaa ttgtagctgc cgaagatcaa
     4681 aactttccaa atcattttgg atttgatttt aaacagatag aaaaagcttt aaaagaaaat
     4741 aaacgcggaa gaagagttcg cggtgcaagc acgattacac agcaggtagc aaaaaatctt
     4801 tttttgtggg aaggaaaaag ttttgtgcgc aaaggaattg aagcatactt tacattatta
     4861 at
//
LOCUS       DASWBY010000905         4969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_335756, whole genome shotgun sequence.
ACCESSION   DASWBY010000905 DASWBY010000000
VERSION     DASWBY010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335756"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VF870_16315"
     CDS             complement(<1..270)
                     /locus_tag="VF870_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253813.1"
                     /translation="MKAIIYSAAVTITVLACSIYSVNAQDASSILKRMDDVMYSPKDL
                     TGKNRIVLIDKNGKEEIREATIQQKGNDKRIFRFTAPASQAGISVL"
     gene            complement(296..2548)
                     /locus_tag="VF870_16320"
     CDS             complement(296..2548)
                     /locus_tag="VF870_16320"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9253814.1"
                     /translation="MERFAQKILKNRWIIILSVIGITVFFGLQTRFLKINSDIISSLP
                     DDDPAALMFKNIGNQFGGNEMGMIVLESDNIFKADLLQHVKQITDSLKITHGVSTVTS
                     LTNILDIKSTEEGIEIGKLVDEYNLPVEQSRLDSLKTYIYSKEMYKGAIVSEDGTATV
                     IMFTLIPDADKQAVANEIKNMVEGLDLPESVYFGGLPFMINDVSDLIVSDIVWLLPIV
                     FIVIALILYISFRSLRGLLMPLLTAGIAVIWTVGIIVTAGHELTIILNIVPVILLAVG
                     SAYTIHVINSFNLFRAGDFNQTITKALGYVIIPVILAALTTAVGFVSFVLGAYLTMIK
                     DFGIFTAIGVIVSLILAIFFVPALTSIFSKNDRKVTVDLPEKKTILSHFILQPLSNLL
                     FKHPKYIFSIWGTFLVISIVGMFLIKTSTNMADYFKKDNPTRISEDLMQKKFGGSFPV
                     FVVFDGDVQSPEVLNLMIKTEHFMKQDPNISITQSVGDLIEQMNDAMLEGKRIPDDQS
                     KIEQLWFLLDGQDVMTQLVSEDLDRGIIQSKFASVENQEIEAFTKKMQLFIEENTSET
                     CKIELTGMPSVYVRLNDSLIKSQFSSLLIALVLVVLIVGLIFRSFSKGVYATIPIIAT
                     ILILFGFMGFTGIPLDIATVLVGSIALGIGIDYSIHFISGFNSHLKENGDAEKAIERT
                     LMTSGKAIIINVTSVAAGFLVLLFSQLVPLQNFGLLVAISMIGSGLGSLTLLPVILIL
                     VNRKRKIVAN"
     gene            complement(2652..3236)
                     /locus_tag="VF870_16325"
     CDS             complement(2652..3236)
                     /locus_tag="VF870_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9253815.1"
                     /translation="MSEINVRNLIVNSASKYFSKFGFHKTTMDEIAKHIHKAKGVLYY
                     YFKGKEELFNEVLKQELNAVKIELKKIVDAENDSLITLKEYTLTRLRLLHKAVNYHET
                     LKADFFEKYLFVKDVREDFTEFEREQLTIILERGKTEGYLDFRNINSTISIFMMVING
                     IEIPLYLQEKYSEYESTIDELSSMIVNSLRTQRK"
     gene            complement(3370..4134)
                     /locus_tag="VF870_16330"
     CDS             complement(3370..4134)
                     /locus_tag="VF870_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253816.1"
                     /translation="MGTNARNTVGLKLIQLSSDTYVIQHQCLLAPMKTVKIGEELLIV
                     YPDTIDPLIYPARLLELWQEGDVLFLKLWNYHKFKWQLYTQDLTTENCLFSFMSIAFI
                     TKLADVMTDLRIHESRMVNKLSQNTKGQSLITNDTLKNKPNLPQPFYNSSGNMVLKYI
                     SESEIAVDLPFILTQNKRLKLNMPYLKTESKLSGNDISAIRLLDFELNEGIIILFVQE
                     LKSKKTYSLEWNLDYAGSYWLWSLSNFETILGITQK"
     gene            complement(4136..4525)
                     /locus_tag="VF870_16335"
     CDS             complement(4136..4525)
                     /locus_tag="VF870_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253817.1"
                     /translation="MKIMDSNYPANLDELVHDMEMHPYQYQHLKIFLRRESNTGIPDF
                     GEQYLAVSHDGFALYRLDSVDYQDGNIILSLTEQSTNQPVKFSIDINNAQPSCIFIRW
                     TDIWNLVLADILRSDVDDIDLIEIIED"
BASE COUNT         1557 a          921 c          825 g         1666 t
ORIGIN      
        1 taatactgat atccccgcct gtgacgctgg tgctgtaaat cgaaatattc ttttatcgtt
       61 tcctttctgt tggattgtcg cctctctgat ttcttctttt ccattcttat caataagaac
      121 aatcctattc ttacctgtta aatctttcgg tgagtacatt acatcatcca ttctttttag
      181 aattgaagaa gcatcctgag catttacaga gtatatacta catgccagaa cagtaatggt
      241 aactgccgct gaataaataa ttgctttcat attttaatgt ttatttgtga ataaattaat
      301 ttgcaacgat tttacgtttc ctgtttacca aaatcagtat tacaggcaaa agtgtcaatg
      361 agccaagtcc agaaccaatc atgcttattg ctacaagcaa tccgaaattc tgtaagggaa
      421 ctaactgaga aaatagcaat accaagaatc cggctgcgac cgatgtgaca ttaattatga
      481 tagccttgcc gcttgtcatt aacgttcttt cgatagcttt ttcagcatcg ccattttctt
      541 taaggtgtga attaaatcct gaaataaaat gtatcgaata atctatgcct attcccaatg
      601 caatactacc aacaaggaca gtagcaatgt caagaggaat tccagtgaat cccataaacc
      661 cgaataagat aagtattgtt gcaattatcg gaatggtcgc atagactccc tttgaaaagg
      721 aacggaatat taatcctaca atcagtacca ccagaaccaa ggctatcaaa aggctgctga
      781 actgactttt aatcagactg tcatttagtc tgacataaac agaaggcata cctgttaatt
      841 caattttgca agtctcactt gtgttttcct caatgaacag ttgcattttt ttggtgaatg
      901 cttctatttc ttggttttca accgaggcaa attttgactg gataattccc ctgtccaaat
      961 cttccgaaac gagttgagtc ataacatcct gcccgtcaag aagaaaccat aactgctcga
     1021 ttttagactg gtcatcaggt atccttttac cttcaagcat tgcatcattc atttgctcta
     1081 tcaagtcgcc aactgattga gttatgctga tattcgggtc ttgtttcatg aagtgttcgg
     1141 tctttatcat gaggtttaaa acctccggac tttgcacatc accgtcaaaa acaacaaaaa
     1201 caggaaatga acccccaaat tttttctgca tcaaatcctc tgaaattctt gttggattat
     1261 ccttcttgaa ataatcagcc atgtttgtac ttgttttaat aaggaacatg ccgacaatgc
     1321 ttataaccag aaaagtgccc cagattgaaa atatatattt agggtgttta aaaagcagat
     1381 tagaaagagg ttgaaggata aaatgagata aaatggtttt cttttcaggt aaatctactg
     1441 ttaccttgcg gtcatttttt gaaaatatgg aagtaagagc cggaacaaaa aatattgcaa
     1501 gaatcagaga tacaattact cctatagctg taaaaatgcc aaaatcttta atcattgtca
     1561 gatacgcacc aagaacaaat gacacaaatc caacggctgt agttaacgca gcaagaatca
     1621 caggaatgat tacatatcca agtgcctttg ttattgtttg attgaaatct cctgctctga
     1681 atagattaaa gctatttatt acatgaattg tgtaagcact tcccactgca agcagaatga
     1741 caggtacaat attcaggata atggtaagtt catgacccgc agtaacaatg attcctacag
     1801 tccatataac ggcaatcccg gcagtaagga gtggcatcag taagcctctg agagacctga
     1861 aacttatgta taaaataagc gcaataacta tgaaaactat tggcagaagc catactatat
     1921 ctgaaacaat taaatcagaa acatcattta tcataaaagg tagtccgcca aaatagactg
     1981 attcaggcaa atccagtcct tcaaccatgt tcttgatttc attggcaact gcctgtttat
     2041 cagcatcagg aattaaagtg aacattataa ctgttgccgt tccatcctct gatacgattg
     2101 cacctttata catctctttt gagtatatat aggttttcag gctgtctaac cgggactgct
     2161 caaccggaag gttatactcg tcaacaagtt taccgatttc aattccttct tcagtgcttt
     2221 tgatatcaag tatgttagtc aggcttgtga cagtagaaac gccatgagta attttcagac
     2281 tgtctgtaat ttgtttaacg tgctgtagca aatcagcttt aaaaatattg tctgattcaa
     2341 gaactatcat gcccatttca ttaccgccaa actggttgcc aatattttta aacatcaagg
     2401 cagccgggtc atcatccggt aatgaactga ttatatcaga attgattttt aagaatctgg
     2461 tctgaagacc aaagaatact gttataccaa ttacacttag tataattatc catctgtttt
     2521 tgaggatttt ctgtgcaaat cgttccatat attaaatatt gactattatt gttttaaagt
     2581 cagttcaaaa ttaaaaatag attttaacta aaaatgtttt ttgttcaaat tttaacaaaa
     2641 aaaatttacg attattttct ttgtgttctt aaactattga caatcattga tgatagctca
     2701 tcaatagtgc tctcgtattc tgaatatttt tcctgaagat ataatggtat ttcaatccca
     2761 ttaatgacca tcatgaatat acttatggtc gaatttatgt ttctgaaatc aagataaccc
     2821 tctgttttcc ccctttcaag tattattgtt aattgctccc tttcaaattc agtaaaatct
     2881 tcacgaacat ccttcacaaa tagatatttt tcgaaaaaat cagccttaag tgtttcgtga
     2941 tagttgacag ctttatgtaa taaccttagc cttgtcaggg tgtattcttt gagtgtaata
     3001 agggaatcat tttcggcatc aacaattttc tttaactcta tcttaactgc gtttaactcc
     3061 tgctttagta cttcattaaa aagctcttcc ttccctttaa aataataata taatactcct
     3121 ttggctttat ggatgtgttt ggcaatctca tccattgtgg ttttatggaa accaaatttt
     3181 gaaaagtact tcgaagctga atttacaatc agatttctta cgttaatttc tgacattata
     3241 tttgactatt ttgattttca ggtcaaattt acaagttttt taattgttat ctcaattaag
     3301 cgacaggatg ctgacaaata aagtgtttaa tgcaaaacta ttggtaatta taattcaaaa
     3361 tttattactt cacttctgcg tgatacccaa gatagtttca aaattactta aactccataa
     3421 ccaatagctc cctgcataat ccaaattcca ttccagtgaa taagttttct tgcttttcag
     3481 ttcctgaacg aataatatga taattccttc atttaattcg aaatccaata acctgatggc
     3541 actaatgtca ttgccagata atttactctc ggttttaagg tatggcatat tcaatttcaa
     3601 tcttttattt tgagtcaaaa tgaacggtag gtcaaccgca atttcagatt cagatatata
     3661 tttcaatacc atattcccag aactgttgta aaatggttgt ggtaggtttg gtttgttttt
     3721 taaggtgtca ttggttatca aagattgtcc tttggtgttc tggctcagtt tattaaccat
     3781 ccgtgattca tgaattctta ggtcagtcat tacgtccgcc aatttggtaa taaaggcaat
     3841 tgacatgaat gaaaagagac agttttctgt tgttaaatcc tgagtgtaaa gttgccattt
     3901 aaacttgtga tagttccata atttcaaaaa gagcacatcc ccttcctgcc acagttccag
     3961 taatcttgcc ggataaatca atgggtcaat ggtatccgga tatactataa gcaactcttc
     4021 cccaattttt acagttttca taggggcaag caagcactga tgttgaatca cgtaagtgtc
     4081 agatgataat tggattagct ttaaaccaac ggtatttctt gcatttgttc ccattctaat
     4141 cttcaattat ttcaatcaag tcaatatcgt ctacgtcact tctcaagata tcagctaaca
     4201 ccaagttcca gatatccgtc cagcgaatga atatgcagct tggttgagcg ttattaatgt
     4261 cgatggagaa tttaactggt tgatttgttg attgttcggt taatgacagt atgatattac
     4321 catcctgata atcaacagaa tctaacctgt ataaagcaaa tccatcatgg ctgactgcga
     4381 gatattgctc tccgaaatcc gggataccag tgttgctttc acgtctcagg aaaattttaa
     4441 gatgctgata ttgatacgga tgcatttcca tatcatgaac aagttcatca aggtttgctg
     4501 ggtaatttga atccattatc ttcatttaaa tttattgctc tatacgggta tgtaccaccg
     4561 taacccccgg taaggggcgt tccccccata gtagtgccaa tataagccgc tggtggggcg
     4621 ggtgtttatc cgcttaactt taatataaat acagaaaaaa atgaattagg gtcgggattc
     4681 aggtcttgaa aaaacttttt aattgaaaaa atctggaaaa aatttttgaa atccagaatg
     4741 ttacaatgtt tcttctttgt aatgaagttg attttttttc aaaaaaaatt tggaaaatat
     4801 accagttgac gaacataatt ttatattgca aattttattg caaataaaaa aggactcgca
     4861 atagttaact ttgcaagtcc ttatatatgc ctgattgtca ggtcttattt tgagcgggaa
     4921 aagggattcg aaccctcgac cctcagcttg ggaagctgat gctctacca
//
LOCUS       DASWBY010000906         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_408421, whole genome shotgun sequence.
ACCESSION   DASWBY010000906 DASWBY010000000
VERSION     DASWBY010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408421"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VF870_16340"
     CDS             complement(<1..502)
                     /locus_tag="VF870_16340"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEX9253818.1"
                     /translation="MRSIKGNFLQLVLFLSISAAALLVFSCSDSTSPETGQGQLKITM
                     VDAPAAYDQINIAVNRVEVHRSGSDSSSGWFVINNNPATYNLLDLRNGASVILGDNSL
                     DAGSYTQIRLIIGTGSNIVVDGITYPLEVPSGEQSGIKLNHEFEIQSGLIYELMLDFD
                     AEHSIVL"
     gene            complement(749..2134)
                     /locus_tag="VF870_16345"
     CDS             complement(749..2134)
                     /locus_tag="VF870_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253819.1"
                     /translation="MNTTDNESKKFDGRKISPRKFVKTISLIVGTIFLVCALSFVFFP
                     DPFINTFVKNKITNAFTEAYPEYSLQIGGMHYSILKNRLECDSIRLKANDSTFTCRLD
                     SFSISGISWMKIITQSGFKPNIISNSIIGAQNIVFSFNKSQDELRIGRLHISLPDSGM
                     LIDSIRFHPLINDEHFFAKSQFRQTRFRLDISQIKIIGFDFLSYLERDTYSAEKIILK
                     DVFADILLNKDKPSDENLANPRMPNEIFSSMKEIICIDSLQIINGQLKYYERVAVNLP
                     PGVVTFSKFKISATGIANHTAQPETTIIRGDGLFMNSAAMKVFMAIPLTSKDFSLHYS
                     GSLGRMNASDLNAFIEPVENRLIKSGVIQNANYNINVNSGNASGNLRVAYDDLAIAIS
                     NKKTGFDLGLLNHISNFIGDRFIIRTTNLPDEKGSMKIGEIKHTRNPGDYFFQYLWFS
                     LRSGVGDVVGF"
     gene            complement(2131..>3041)
                     /locus_tag="VF870_16350"
     CDS             complement(2131..>3041)
                     /locus_tag="VF870_16350"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9253820.1"
                     /translation="EKHIPSGFAVFIVMTGMISIIILIGAQIATSFSSFLTELPSLQS
                     RIREQVSDLTAIISSKDFVIKEKYFLDYINPESIMKLTANLLSGFSSVLSDLVLILMT
                     VTFILLEVSSFPIKLRKILGDPKQKFPQFTKFSDDMRRYMVIKTLISLSTGILIAFWL
                     YIFGVDYPILWGFLAFLFNYIPNIGSMIAAIPALVLAFIQLGIGSTLIVALGYIAVNF
                     IIGNVIEPRLMGRRLGLSTLVVFLSLIFWGALLGMVGAILSIPLTMTLKYAFENHEST
                     KWIAVLLESEKVDEQAGSFLKRKKKS"
BASE COUNT         1036 a          539 c          493 g          973 t
ORIGIN      
        1 taagtactat tgaatgctcg gcatcaaaat caagcatcaa ttcgtaaatg agccctgatt
       61 gaatttcaaa ttcgtgattt aattttatac ctgattgttc tccacttgga acctctagag
      121 gataagtaat tccatcaaca actatattgc ttcctgtacc aattattaat cttatttgcg
      181 tgtaggaccc ggcatcaagt gaattatcac caaggataac acttgcaccg tttcttaaat
      241 caagcaaatt ataagttgct ggattattgt ttataacaaa ccaaccactg cttgaatcgg
      301 aacctgatct gtgaacttca acccgattaa ctgcaatatt aatctgatca taagctgcag
      361 gtgcatcaac catagttatt ttcaactgtc cctgtcctgt ttcgggtgaa gttgaatctg
      421 aacagctaaa gacaagcaaa gccgctgctg aaatgcttaa gaatagaact aattgtaaaa
      481 aatttccttt aattgatctc atcgaaaaaa ctccttaata aataaattaa aataaaataa
      541 atctaacttt tggttaagat tgttctaaag gattctggta aatcgtctta ttgaacgtga
      601 agtaaactac tctactatgg ctgggtaatc aataaggcga agggatgaaa aaaggattag
      661 acctttttat ttggattctt ttcaagaagt aattaaaaat aatttgtgac atatttttat
      721 taaacaaaaa ttaaatgact aggcatcttc aaaatccaac aacatccccg acaccgcttc
      781 gaagagaaaa ccataaatat tggaaaaaat aatcaccagg atttcttgtg tgttttattt
      841 caccaatttt cattgagcct ttttcatcgg gcagattggt tgtgcgtata ataaacctat
      901 cgccaataaa atttgaaatg tgatttaaaa gtcctaaatc aaatccggtt tttttatttg
      961 aaatagcaat tgccagatca tcgtaagcaa ctcgcaaatt accgcttgca ttgcccgaat
     1021 taacattgat attataatta gcattttgaa tgactcctga ttttatgagt ctgttttcaa
     1081 caggctcaat aaatgcattt aaatctgaag cattcattct gccaagtgaa cctgaatagt
     1141 gcagagaaaa atcttttgaa gttagcggaa ttgccataaa cactttcatc gcggctgaat
     1201 tcataaaaag cccatctcca cgaataattg tagtttcagg ttgtgctgtg tgatttgcaa
     1261 ttccggttgc ggatattttg aatttactga atgtaacaac ccccggtggt aaattaacag
     1321 caactctttc ataatatttc aattgcccat ttataatttg taaactatct atgcatatta
     1381 tttccttcat tgaagaaaat atttcattcg gcattctagg gttggctaaa ttttcatcgc
     1441 ttggtttatc tttgtttaaa agaatatcag caaaaacatc cttcagaata attttttcgg
     1501 cgctgtaggt atctcgctca aggtacgata gaaaatcaaa acctattatt tttatttgag
     1561 aaatatcaag acgaaacctt gtctgcctga attgactttt cgcaaaaaag tgctcatcgt
     1621 taattaaagg atggaacctg atcgaatcaa tcaacattcc agaatcaggc aatgatatat
     1681 gcagccttcc tattctaagt tcatcctgtg acttattaaa actgaataca atattttgag
     1741 cgccaattat tgagtttgaa atgatgtttg gtttaaaacc gctttgggta ataattttca
     1801 tccagctaat tccgctaata gaaaacgaat caaggcggca ggtaaaggtt gagtcattag
     1861 cttttaatct tatagaatca cactctaacc tattcttcaa gatactataa tgcatcccgc
     1921 caatctgtaa agaatattcc ggataagctt ctgtaaatgc gttagtaatt ttgtttttta
     1981 caaatgtgtt gataaaagga tccggaaaaa aaacaaagct taatgcacag acgagaaata
     2041 tagtgccgac aataagacta atggtcttta caaatttccg cggcgatatt tttctcccat
     2101 cgaacttttt tgattcatta tctgttgtgt tcatgatttc ttttttcttt tcaaaaatga
     2161 accagcttgt tcatcaactt tttccgattc gagtaaaaca gcaatccatt tagtgctttc
     2221 atgattttca aatgcatact tcaaagtcat agttagtggt atagagagaa ttgcacctac
     2281 cattccaagt aaggctcccc aaaaaataag tgaaagaaaa actactaatg tagagagtcc
     2341 aagccttctc cccatcagtc gaggttcaat aacattacct attataaaat taactgctat
     2401 atatccaagt gcaacaatca aagtacttcc aattcctagt tgaataaaag ctaaaaccaa
     2461 agcaggaata gcagcaatca tagatccaat gttaggaata tagttaaaca gaaaagccag
     2521 aaagccccag agtattggat agtcaactcc gaatatatac aaccagaacg ctattaaaat
     2581 acccgtggac aaactgataa gagttttaat taccatatat cgcctcatat catcagagaa
     2641 ctttgtaaac tggggaaatt tttgttttgg gtcaccgagt atttttcgga gctttattgg
     2701 aaagcttgaa acttcaagca aaataaaagt tactgtcatt agaattagaa caagatcaga
     2761 aagtacagaa ctaaatcctg ataataagtt tgctgttagt ttcattattg attcgggatt
     2821 gatgtaatct aaaaaatatt tttccttaat aacaaaatcc ttactggata taattgccgt
     2881 gaggtctgaa acctgctctc gaatgcgaga ttgcaatgat ggtaattctg ttaaaaagct
     2941 gctaaaagat gttgcgattt gagcgccgat taaaattata atggaaatca ttccagtcat
     3001 tacaatgaac accgcaaatc cggaaggaat atgtttttct t
//
LOCUS       DASWBY010000907         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_408456, whole genome shotgun sequence.
ACCESSION   DASWBY010000907 DASWBY010000000
VERSION     DASWBY010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408456"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..893
                     /locus_tag="VF870_16355"
     CDS             117..893
                     /locus_tag="VF870_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253821.1"
                     /translation="MKNFISLLIIFVLSLQLNYAQGTAGENAKYEYRQLIDMPTAGVI
                     QKGSFGLSTELLPLGTLIVKVEAGIIENLSIGLSYGGSNIIGSGDINWYKFPPGVNMR
                     FRLFDESLVIPALTLGFDTQGKGEYFDDVKRYEIKAPGFFAAACKNFSILGYLSLHGE
                     INYSILEDKDGDNFINLMVGAEKTIGSSFSVLAEYNFAFNDNSTNEFGEGKGYLNMGI
                     RWSIADGVTFGFDLRDLLGNSSFDTNAADRGLVIEFIQKI"
     gene            1059..>1699
                     /locus_tag="VF870_16360"
     CDS             1059..>1699
                     /locus_tag="VF870_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253822.1"
                     /translation="MKTYILIFLSLTTLLIMTGCYSQLATSESDYGNWSREQDNNDYQ
                     AASSEAMDSLGVDDYAAQYDESYVDSEGNVTNYYFYGYPSYRKYFWDYYPAVSISIAA
                     GWYYDPWYWYSWYPSYWYYPSVYYYYPTYWYPNYCYGYYDTYPYYDNGYYDTYKYRTN
                     DGYKIRNNSGGRNSGGDRNLYTSTDRTSTLRDQLKTREKQIVSGRDDKNRELTG"
BASE COUNT          546 a          257 c          329 g          567 t
ORIGIN      
        1 tctctgttgc ctgaatatat tgaaatcgaa atacttgctt taactgtttg aattttaagc
       61 atcaaagaaa aattattttt attttgtggt taagttatta atccaaacga ggaaaaatga
      121 aaaattttat atcattatta ataatctttg tcctatctct gcaattaaac tacgcacaag
      181 gcacggccgg agaaaatgct aagtatgaat acaggcagtt aatcgatatg ccgacagcag
      241 gtgtgataca gaaaggttct tttggtttaa gtactgaact gctgccgctt ggaacgctta
      301 ttgtgaaagt tgaagccgga atcattgaaa atcttagcat cggactttct tacggcggct
      361 caaatataat tggctccggt gacatcaatt ggtacaaatt tcctccgggt gttaatatgc
      421 gattccgttt atttgatgaa tcgctggtaa tacctgctct tactcttgga tttgatacgc
      481 aaggaaaggg agaatatttt gatgatgtga aaagatatga gataaaagct cccggatttt
      541 ttgccgctgc ctgcaaaaat tttagcattc ttggatattt aagcttacac ggagagataa
      601 actattcaat tcttgaagat aaagatggag ataattttat caatttaatg gtcggtgcgg
      661 aaaaaactat tggaagttct ttctcggtgc ttgccgaata taattttgct ttcaatgata
      721 attcaaccaa tgaatttggt gaaggcaaag gctacttaaa tatgggtatt cgttggtcaa
      781 ttgctgacgg agttactttt ggattcgatc ttagagactt acttggaaac agttcttttg
      841 atacaaatgc tgcagataga ggattagtaa tcgaattcat tcagaagata taaattaaac
      901 tgattcttta tgtgtttttt ttagtacaaa atagtttact ccagtataca cttttcattt
      961 aagagatacg attttttagc aatccggtat ttatttaacc acttttttaa ggctcgatat
     1021 ttgcaatttt tgcattaatt gaaatccaga ggtttaaaat gaaaacatat attttaattt
     1081 ttttgtcact tacaacactc ttgataatga caggatgtta tagtcagctt gctacttcag
     1141 agtcagatta tggaaactgg agtcgtgagc aggataataa cgattatcaa gctgcaagtt
     1201 ctgaagcgat ggacagttta ggtgttgatg actacgcagc tcaatatgat gaatcatatg
     1261 ttgatagtga aggcaatgtt accaattatt atttctatgg atatccatca tacagaaagt
     1321 atttctggga ttattatccg gctgtatcaa tatcaatagc tgccggctgg tattatgatc
     1381 cttggtactg gtattcatgg tacccaagtt attggtacta tccatccgtt tattactatt
     1441 atccaactta ctggtatcca aattattgtt atggatatta tgatacatat ccatactatg
     1501 acaatggtta ctatgatact tataaatacc gaacgaatga tggatataag atcagaaata
     1561 attccggagg cagaaacagc ggaggagata gaaatttata tacttccacc gacagaactt
     1621 caacattgag agatcaactt aaaacaagag agaaacaaat tgtatccggg cgtgatgata
     1681 aaaatcgtga attgactgg
//
LOCUS       DASWBY010000908         5129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_408531, whole genome shotgun sequence.
ACCESSION   DASWBY010000908 DASWBY010000000
VERSION     DASWBY010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408531"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VF870_16365"
     CDS             complement(<1..930)
                     /locus_tag="VF870_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9253823.1"
                     /translation="MQIEFIGGARTVTGSQHLLHINGKKILLECGLFQGRRSETYEKN
                     KNFKFNPAEIDALILSHAHIDHSGNIPNLVSKGFDGLIYATSATVDLCQIMLRDSAHL
                     QEKDIEYVNKKKNKKGEEPFEPLYTLEDVEKAMQQFVGVQYNRTIDLFPGIKFMFRDA
                     GHILGSAGVRLEIEEAGGKKISLGFSGDIGRPDSPVIKSPDVLRDLDVLIMESTYGNR
                     LHSPAEEVEEDFVSTINPVINRGGKIIIPAFAVGRTQTIVYMLHKLFDQNRIPEVPIY
                     VDSPLAVEATNIFRSHPECLDRETYRIFLQNGED"
     gene            complement(981..1556)
                     /locus_tag="VF870_16370"
     CDS             complement(981..1556)
                     /locus_tag="VF870_16370"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX9253824.1"
                     /translation="MTKNRLEAFSDGVLAIIITIMVLELKVPHDPSLKALLPILPVFL
                     SYILSFIYVGIYWNNHHHLLHTVSKINGGILWSNLNLLFWLSLLPFVTGWMGENHFAL
                     VPTAFYGLILLMAAIAYYILQLTIINQQGDNSILAKALGTDLKGKLSIVIYLISISLV
                     FINPFISQGLYALVAFLWVIPDKRIERVLSE"
     gene            complement(1584..2123)
                     /locus_tag="VF870_16375"
     CDS             complement(1584..2123)
                     /locus_tag="VF870_16375"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX9253825.1"
                     /translation="MIKYIAFLKAINVGGHTVKMDNLKKLFEKMGFEKVETFIASGNL
                     VFETKSKSMDSIKKKIESELEKSLGYNVPTFIRTTGEIKTILEYKPFKESEINNPQNS
                     LYIGFLDKAPSKENQNKLYSLQDEANQFHFNKKELYWLCRKRFSDSGITGNKLEKALG
                     MKTSVRNSTTIRKMASKFS"
     gene            2304..3611
                     /locus_tag="VF870_16380"
     CDS             2304..3611
                     /locus_tag="VF870_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9253826.1"
                     /translation="MKYILIFSIFAVSLFAQVNAGDVKSFTLKNGMKIFVLEDNSIPN
                     ANMYFFYKVGSRNEYIGITGISHFFEHMMFNGAKKYGPKEFDRVMEANGGSNNAYTSE
                     NITAYTDWFPSSSIEVMFDLEADRIANLNFDPKMIESERGVILSERSTGLENNPLEQL
                     WQEVQATAYIAHPYMWPVIGWESDIKNWTKEDLENYFHTYYAPNNCVVVISGDVKFNE
                     VEILAKKYFEPIPAGPKPRSLHTIEPEQTGERRVFVKREVPNPYLMITYHVPQSGSED
                     YYALDLLNSILSEGESSRLYNSIVDNQQLALEVGSFYPDAFDPTLFYFYGICNDGIAA
                     SQLEKAVVDEVDKIIKDGISENELQKVKNQKLMSFYKTTETINGMSNTIGTYELFFGD
                     YKKMFTAPDAYKKVTKEDIQRVAAKYLTKQNRTVGILNTEEAQ"
     gene            3608..4987
                     /locus_tag="VF870_16385"
     CDS             3608..4987
                     /locus_tag="VF870_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9253827.1"
                     /translation="MKRISLILFISIVLVSLTSAQFKLPKYEVAALDNGLTIYLMEKK
                     DVPIISFSVVFDAGAVRDGAQNGLASFTAEALKFGTANYSKNQIDSIFNFYGSSLNTY
                     ARLDYSGLYTQFMKDNFDQLLPVIKEVMTSPAFPEEEIVKRKQRWIAELDQAKESPRQ
                     VIGSYYNKFLFQDSPYGNPVDGTKKTIELIDRKAISTFYSENIRPENCALAIVGDFDL
                     NKMKSELTEYFAGWSVSTPRLINDLRFINQELKEPAVYLINKDNARETTFMIGGFGVP
                     MNNQDQIQIDVINTILGGRFTSWLNDELRVNAGYTYGARSRFASYRTAGTFYISTFTA
                     TKNTEAAIDLALKTYNRLFEKGIDEATLKSAKNYVKGQFPPEYETAGSLAGFLTQKYI
                     YGLDDSYINDFEKAVDGMTVEKANEIIKKYFPKNNLQFVLIGKTDNIRETAMKYGRVI
                     EKNIGEDGF"
BASE COUNT         1728 a          866 c          883 g         1652 t
ORIGIN      
        1 gtcttcgcca ttctgtaaaa agatacgata tgtttctcta tcaaggcact ctgggtgcga
       61 acgaaaaata tttgtagctt caaccgctaa gggtgaatcc acgtaaatcg gtacttcagg
      121 aattctattc tgatcgaaaa gtttatgaag catgtaaaca atagtctggg ttcttccaac
      181 ggcaaatgcc ggaataataa tcttgccgcc tctgttaata accggattta ttgttgatac
      241 aaaatcttct tcaacttctt ctgccggcga gtgtaatctg tttccataag ttgattccat
      301 aatcaacaca tcaagatccc gcaaaacatc gggagatttt attacgggtg aatctggcct
      361 tcctatatct cctgaaaatc ctaaactaat ttttttacct cctgcttctt ctatttctaa
      421 acgcacacct gcagaaccaa gaatatgtcc cgcatctcta aacataaatt ttattccggg
      481 aaacaaatca atcgttctgt tgtattgaac tccaacaaat tgctgcatag ctttttcaac
      541 atcttccaat gtataaagcg gctcaaatgg ttcttctccc tttttgttct ttttcttatt
      601 tacatactca atatcttttt cctgcagatg tgcagaatca cgaagcatta tctgacacaa
      661 atctacggtt gcagatgttg cataaatcaa tccatcaaac cctttgctta caagatttgg
      721 gatgtttccg ctgtgatcga tatgcgcgtg tgaaagaatt aatgcatcta tttctgcggg
      781 attaaatttg aagtttttat ttttttcata cgtctcactt ctcctgccct gaaataaacc
      841 gcattcaagt aaaatctttt ttccgttgat gtgaagtaaa tgctgcgaac ctgtaacggt
      901 tcttgcgccg ccgataaatt ctatttgcaa aataattctc ccgaagatct ataattaaaa
      961 atttgaaaaa aatatactta ttattctgat aaaactcttt cgattcgttt gtcaggaatc
     1021 acccacaaga atgcaaccaa cgcatacaag ccttgtgaaa taaaagggtt tatgaagacc
     1081 aaggaaatgg atatcagata aataacgata gacaactttc ctttaagatc ggttcccagg
     1141 gctttggcaa gaattgaatt atcgccctgt tgattgatga ttgttaattg caaaatataa
     1201 taagcgattg ctgccattaa tagtattaac ccataaaatg cagttggcac aagtgcaaaa
     1261 tggttttctc ccatccatcc agttacaaat ggaaggagag ataaccaaaa cagtaaattt
     1321 aaatttgacc acaaaattcc accattaatc ttgctgactg tgtgaagtaa gtgatgatgg
     1381 ttgttccaat aaattccaac gtaaataaaa cttaaaatgt agcttaagaa aaccggcaat
     1441 attggcaaca aagctttcaa actgggatcg tgcggaacct ttaattccag caccataata
     1501 gtgattatta tagccaaaac gccatcacta aaagcttcta aacgattttt tgtcatatgt
     1561 taaattatac ttaaaagaaa ttattaagaa aactttgacg ccatttttct tattgttgtg
     1621 gagttgcgca cagatgtctt cattccaaga gctttctcaa gtttatttcc cgtaattcct
     1681 gaatcactaa atctttttct gcataaccaa tagagttctt ttttattaaa gtgaaattga
     1741 ttggcctcat cctgtaacga atataattta ttctgattct ctttagacgg cgctttatct
     1801 aaaaatccaa tatataaaga attttgggga ttatttattt cggattcttt aaagggtttg
     1861 tattctaaaa tggttttaat ttcaccggtt gttcttatga atgttggaac attataacca
     1921 agagattttt ctaattcgct ttctattttc tttttgatag aatccatact ttttgatttt
     1981 gtttcaaaca caagatttcc gcttgcaata aaggtttcaa ctttttcaaa acccattttt
     2041 tcaaaaagtt ttttcaggtt atccatttta acggtgtgcc cgccaacgtt tatagcttta
     2101 agaaatgcga tatattttat cattattgtt ttttgtcgtt aataattatt cattacaaag
     2161 attaatataa aaatataact cttattattt aaaacaatac tggtttgcga atgaaaaatt
     2221 aaaaccactc atcaagttat tctttatata cattatacaa ttcgtatttt agaacactta
     2281 tttaaataat ttttggaata tttatgaaat acattctaat cttttccatc tttgcggttt
     2341 cattgtttgc acaagtgaat gcaggtgatg ttaaatcctt tacgcttaag aacggaatga
     2401 aaatttttgt tcttgaagac aactcaatac caaatgcaaa tatgtacttc ttttataaag
     2461 tcggatcacg aaatgagtac attggaataa ccggcatctc acatttcttt gagcacatga
     2521 tgtttaatgg ggcaaagaaa tatggaccaa aagaatttga ccgtgttatg gaggccaatg
     2581 gcggatcaaa caatgcttat acttcagaaa acataacagc ttacacagat tggtttcctt
     2641 caagttcaat cgaagtgatg tttgatcttg aggctgacag aattgcaaac ctaaacttcg
     2701 atcctaaaat gattgaaagc gaacgcggag taattctttc ggaaagaagt acgggtcttg
     2761 aaaacaaccc tttagaacag ctctggcagg aggtacaagc cactgcatat atagcacatc
     2821 catatatgtg gccggtaata ggatgggagt ctgatataaa aaactggact aaagaagatt
     2881 tagaaaatta ttttcatact tattacgcac caaataattg tgttgttgta attagcggcg
     2941 atgtaaaatt taacgaagtt gaaattcttg caaaaaaata ctttgaacct attcccgcag
     3001 gaccaaagcc aagaagcctg cacacaatag aaccagagca aaccggcgaa cgaagagttt
     3061 ttgttaagcg cgaagttcct aatccatact tgatgattac ttatcacgtc cctcaaagcg
     3121 gcagtgaaga ttattatgca cttgacttat tgaactcaat tttatcagaa ggcgaatcct
     3181 caagacttta taattcaatt gtagataatc aacaattagc attagaggtt ggaagttttt
     3241 atccggatgc ttttgatccg acactgtttt atttttatgg aatttgcaac gatggtatag
     3301 cggcatcgca attagaaaaa gctgttgtgg atgaagttga taaaatcatt aaagatggaa
     3361 taagcgaaaa cgaacttcag aaagtcaaaa atcaaaagct aatgagcttt tataaaacaa
     3421 ctgaaactat aaacggaatg tctaacacta tcggcaccta tgaattattt ttcggcgatt
     3481 ataaaaaaat gtttaccgca ccagatgctt acaaaaaagt taccaaagaa gacattcaaa
     3541 gagttgccgc aaaatatctt accaaacaaa acagaacagt tggaattttg aatacggagg
     3601 aagcacaatg aaaagaattt cattaatatt atttatcagt atagttcttg tttctttaac
     3661 aagcgcacag ttcaaacttc caaagtatga ggttgctgcc cttgataatg gattaacaat
     3721 ctatttgatg gaaaagaaag atgttcctat aatttctttt tctgtcgtgt ttgatgccgg
     3781 cgcagtaaga gatggtgcac aaaacggatt agcttcattt actgcggaag cattaaagtt
     3841 tggaacagcc aattattcaa aaaatcaaat cgattctatt tttaattttt acggaagcag
     3901 tttgaatact tatgcaagat tggactactc cggtttatat acccaattta tgaaagacaa
     3961 ttttgatcaa cttcttcctg ttataaaaga ggtaatgaca agccctgctt ttcccgaaga
     4021 agaaattgtt aaaagaaaac aaagatggat cgctgaactt gatcaggcaa aagaaagtcc
     4081 acgacaggta attggaagtt attacaataa atttttattt caagattctc cttatggtaa
     4141 cccggttgac ggaacaaaaa aaacaattga attgatagac aggaaagcaa tttctacttt
     4201 ttattcagaa aacatcaggc ctgaaaattg tgccctggcg attgttggtg attttgattt
     4261 aaataaaatg aaatctgaat taactgaata ttttgcaggg tggagcgttt ctactccaag
     4321 acttataaat gacttaaggt ttataaatca ggagttaaaa gagcctgctg tttatttgat
     4381 caataaagat aacgctagag aaactacttt tatgatcggc ggatttggtg ttccaatgaa
     4441 caatcaagat caaattcaga tagatgtaat caatacgatt cttgggggca gatttacttc
     4501 ctggctaaat gatgagcttc gggtaaatgc aggatatact tatggtgcaa gaagcagatt
     4561 tgcatcatac agaaccgcag gtacctttta tatcagcacc tttacggcaa ctaaaaatac
     4621 tgaggcggct attgatcttg ctttaaaaac gtataacaga ctttttgaaa aaggtattga
     4681 tgaagcaact ttaaaatcag caaagaatta tgttaaagga caatttcctc cggaatatga
     4741 aactgccggt tcgcttgctg gatttttaac acaaaaatat atctatggat tagatgactc
     4801 ctacataaat gattttgaaa aagcagttga tggaatgact gttgaaaaag caaatgagat
     4861 cattaaaaaa tattttccta aaaacaatct gcaattcgtg ctgattggaa agacagataa
     4921 cattcgtgaa acagcgatga agtacggaag ggttattgag aagaacattg gtgaggatgg
     4981 attttagtcg attaatattt ttgaattagg aattaaaagc acgaaagaat ttttttcgtg
     5041 cttttttatt taaagtaaaa agttatcgca tcaacataaa ttttaatttt acttagtatc
     5101 ggattttttg tggaaacggt tttatctcc
//
LOCUS       DASWBY010000909         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_409012, whole genome shotgun sequence.
ACCESSION   DASWBY010000909 DASWBY010000000
VERSION     DASWBY010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409012"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1647
                     /locus_tag="VF870_16390"
     CDS             190..1647
                     /locus_tag="VF870_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9253828.1"
                     /translation="MARLLILGAGIAGQTAALHAKNKLGNKHEVVVISPNSKWNWIPS
                     NIWVGVGRMKPEQVTFQLAPVYKKTGITFYQAKAVSIHPEGDQSSQSPFVTIEYTSPE
                     KTGTQEKITYDYLINATGPKLKFEATKGLGPNFNSLSVCTYDHAAQTSKALDELTEQM
                     KRGEKKNIVIGTGHGNCTCQGAAFEYLFNVEYELRKRNVRDKANIIWISNEQELGDFG
                     IGGMHITRGGYVTHSRVFTESLYTERGINWITRAHVKEVEKNKLIYENLDGEEKMVSF
                     DFAMLLPPFSGVGMKAFNKSDEDISSDLFAANGFMIVDADYSKKTYDDWSPADWPSTY
                     QSPKYKNIFAVGIAFAPPHAISKPMTSPNGTPIFPTPPRTGMPSGVMARQAAYNIADM
                     MNGKANEPTRKASLANMGAACIASAGAGFFSGTAVSMTMYPIIPDFKKYPDTGRELKY
                     TSGEIGLAGHWIKHFLHYAFIYKAKANPFWRVVPE"
     gene            1719..1865
                     /locus_tag="VF870_16395"
     CDS             1719..1865
                     /locus_tag="VF870_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253829.1"
                     /translation="MNERKPYEQNYYPPVPTGFTKFMRTNLIWQFFRFLVINIKMLRM
                     VRKH"
     gene            complement(1872..2372)
                     /locus_tag="VF870_16400"
     CDS             complement(1872..2372)
                     /locus_tag="VF870_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9253830.1"
                     /translation="MYRNLEDFINDWTYESESTLKIFNNLTDESLTKKISENVRTAER
                     LAWHITTSIGEMANRTGLTFKTVDEKSEVPLSAKEIVNAYKETSENLLTSIKSNWNDE
                     TLLIEDDMYGEKWKRGTTLGVIISHQIHHRAQLTVVMRLAGLKVPGVYGPAKEEWVQY
                     GMPAQE"
     gene            complement(2476..>4218)
                     /gene="valS"
                     /locus_tag="VF870_16405"
     CDS             complement(2476..>4218)
                     /gene="valS"
                     /locus_tag="VF870_16405"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX9253831.1"
                     /translation="HKLEMINIFNDDATTNGNVPEEFRELDRYEVRKKVVARLEELGL
                     MFKIEDYQNKVGYSERGNVPIEPYLSEQWFMKMDDLAKPAVDVVHEGKVKFYPKHWEK
                     TYFHWMEGIRDWCISRQLWWGHRIPVWYHNTTHEIYCDVNPPKDLQNWRQDEDVLDTW
                     ASSWLWAHAIFKTDEERKYYYPTNTLVTGPDIIFFWVARMIMAGMHFQKDIPFSDVYF
                     TSIIRDSQGRKMSKSLGNSPDPLNVIKDYGADALRFTVIYLAPLGQDVLFSTDKCELG
                     RNFANKIWNAGRFLLMNAENIPAKKELINSHIDFADEWIISRLNKTLQQLNKAMDEFE
                     VNNAIKLIYSFIWNDFCDWYVEMIKNRLYADDEEIKSAVLTRAIAIFEDALKMLHPFM
                     PFITEELWQLMDERKDSESISTSSYPVADENLIRDSADEEMELVKDIITAIRNIRGEM
                     NIAPSKKVNAMIKSSSVKEYQIEYIKKLAKVEDLKVDANIQKPKASASAVLRGVEIFI
                     PLEGLIDLEVERQKIQKEITRLEGSLAGIEKKLSNEKFVANAAPEVVEKERAKQKDWL
                     DNIGKLKEILANLN"
BASE COUNT         1327 a          800 c          699 g         1392 t
ORIGIN      
        1 cttgcaatta aagatgtaat caatctaagc tttgataaaa aatatgtctg gaaataatgt
       61 gccggtttag attcattatc acccaaaatc aacaaaaata atttacaaac ctaatatttt
      121 atcaaagtat tatttaattt actgccataa ttgtttttgt gttcctaaaa attaatctta
      181 ggagtaacaa tggcccgctt acttatactt ggtgctggaa tagccggaca aacagcggca
      241 ctccacgcaa agaacaaact tggcaataaa cacgaagttg ttgttatctc acctaacagc
      301 aaatggaatt ggattccatc aaacatttgg gttggcgttg gcaggatgaa acctgaacaa
      361 gttacttttc aacttgctcc tgtttataaa aaaacaggaa tcacatttta tcaggctaaa
      421 gctgtaagca tacatccgga aggtgaccaa tcttcacaat ctccttttgt tactattgaa
      481 tatacatcac ccgaaaaaac aggtacgcaa gaaaaaataa cttacgatta tcttatcaac
      541 gcaacgggac caaaattgaa atttgaagca accaaagggt tgggaccaaa ttttaatagt
      601 ttatctgttt gtacttatga tcatgcagcg caaacatcaa aagcgttgga tgaacttaca
      661 gaacaaatga aacgcggtga aaagaaaaat attgtaattg ggaccggaca tggtaattgt
      721 acctgccagg gtgcagcttt tgagtatcta tttaatgttg aatatgaatt aagaaaaaga
      781 aatgttagag ataaagcaaa cattatatgg atatctaatg aacaggaact tggtgacttt
      841 ggaatcgggg gaatgcacat cacacgcggc ggttacgtaa ctcactccag ggtttttact
      901 gaatctcttt acactgaaag aggtatcaac tggattacac gggcacacgt aaaggaagtt
      961 gaaaagaata aattaattta tgaaaatctt gatggcgaag aaaaaatggt tagttttgat
     1021 tttgcgatgc tgcttccacc tttttctggt gttgggatga aagcatttaa taaatccgat
     1081 gaagatattt catcagattt atttgctgcc aatggattta tgattgttga tgcagattac
     1141 tcaaaaaaaa cttatgatga ctggagtccc gctgattggc cctcaacata tcaatcgcca
     1201 aagtataaaa atatttttgc agttggaatc gcctttgcac caccgcacgc aatatcaaaa
     1261 ccaatgacaa gtccaaatgg aactccaata tttccgactc caccaagaac cggaatgcct
     1321 tcaggcgtaa tggcaagaca agccgcttat aacattgcag atatgatgaa tggaaaagca
     1381 aatgagccaa caagaaaggc ttcacttgct aatatgggcg cagcttgtat tgcttcagct
     1441 ggcgctggat tttttagcgg cactgctgtt tcgatgacga tgtatccaat tattcctgat
     1501 tttaaaaagt atcctgatac cggaagagag ttaaaatata cttcgggaga aatcggctta
     1561 gccggacact ggataaaaca ctttttacat tatgcattta tctataaagc aaaagcaaat
     1621 ccattttgga gagttgtgcc tgaatgatat tttaatgata gtttaaaact gtcacactta
     1681 aacttcgctc agtgtgacat taaaattttg gagattttat gaacgaaaga aaaccatacg
     1741 aacaaaatta ttatccgcct gtgccgactg gttttacaaa atttatgaga actaatttaa
     1801 tctggcagtt cttcagattc ctggttataa atattaaaat gttaagaatg gttaggaagc
     1861 actaggagtt tttattcttg tgccggcatt ccgtattgca cccattcttc tttagctggt
     1921 ccgtaaacac ccgggacttt taagccagct aatctcatca caacagtaag ctgtgcacga
     1981 tgatgaattt gatgcgagat gataacgcca agagtagttc ctcttttcca tttttcaccg
     2041 tacatatcat cttcaattaa taaagtttca tcattccaat ttgatttaat agatgtaagt
     2101 aaattttcag aagtttcttt atatgcatta acaatttctt ttgcagataa cggaacttca
     2161 gatttttcat caactgtttt aaaggtcagt cctgttctat tagccatctc tccaattgaa
     2221 gttgtaatgt gccaggcgag tctttctgct gttcttacat tctcactaat tttttttgtt
     2281 aaagattcat ctgtgagatt gttaaatatt tttaatgttg attcagactc ataagtccaa
     2341 tcgtttataa agtcttctaa attacgatac ataattattt acccgattct tagtcacgtt
     2401 tcgacaggct caacgggaca atgtttttac aaatacactg atgtcatcct tagcttgtcg
     2461 aaggataaca gaaaattaat ttaaatttgc taatatctct tttaacttgc cgatattatc
     2521 taaccaatct ttttgcttag ctctttcttt ttcaacaact tcaggggcag catttgcaac
     2581 aaatttttcg ttggagagct tcttttcaat tccggctagt gatccttcaa gtcttgttat
     2641 ttctttctgg attttctgtc tttcaacttc aagatcaatc aaaccttcaa gcggaataaa
     2701 tatctctact ccccttaaaa ctgctgatgc acttgctttc ggtttttgaa tatttgcatc
     2761 gacttttaag tcttcaacct ttgcaagttt tttaatgtac tcaatctgat attctttaac
     2821 tgaagaagat ttaatcattg catttacttt tttagaagga gcaatgttca tctcgccgcg
     2881 aatatttctt atagcagtaa ttatatcttt aacaagctcc atttcttcat cagcagaatc
     2941 tcttataaga ttttcatcag caaccggata agatgatgtt gaaatactct cactatcttt
     3001 tctttcgtcc atcaattgcc agagctcttc tgtaataaac ggcataaatg gatgaagcat
     3061 tttaagtgca tcttcaaaaa ttgctatggc acgagtgaga acagctgatt tgatttcttc
     3121 atcatcagcg taaagcctgt tcttaatcat ttcaacatac caatcacaga agtcattcca
     3181 tataaaactg tagataagct ttattgcatt gttaacttcg aactcatcca ttgctttgtt
     3241 tagctgctgc aaagttttat ttaatcttga aattatccat tcatctgcaa aatcgatatg
     3301 agaatttatt aattcttttt tagctggaat attttctgca ttcattaaaa gaaatcttcc
     3361 ggcattccag attttgtttg caaagtttct tccaagctca catttatcag tactgaaaag
     3421 aacatcctgc ccaagcggcg caagataaat tactgtaaag cgaagagcat cagcaccata
     3481 atctttaata acattaagtg gatcaggaga attgccgaga gatttactca tctttcttcc
     3541 ctgtgaatca cgaatgatac ttgtaaagta aacatcactg aaaggaatat ctttttgaaa
     3601 atgcattccg gccataatca ttcttgcaac ccaaaagaaa attatatccg gtcctgtaac
     3661 aagcgtattt gttggataat aatattttct ttcttcatca gttttaaata ttgcgtgcgc
     3721 ccacaaccag cttgaagccc aggtatcaag tacatcttca tcctgtctcc aattttgtaa
     3781 atccttaggc ggattgacat cacaatatat ttcatgtgtt gtgttatgat accacacagg
     3841 aattctatgt ccccaccaaa gctgacggga aatacaccaa tcgcgtattc cttccatcca
     3901 atgaaaataa gttttctccc aatgcttagg ataaaatttt acttttcctt catgtacaac
     3961 atcaacagcg ggttttgcaa gatcatccat cttcataaac cattgttcgc ttaagtacgg
     4021 ctctatagga acgttgcctc gttcagagta tccaacttta ttttgataat cttctatctt
     4081 aaacattagt ccaagttctt ctaatctggc aacaactttt tttctaacct cataacgatc
     4141 aagttcacga aattcttccg gcacgtttcc atttgttgtt gcgtcatcat taaagatgtt
     4201 tatcatttca agtttatg
//
LOCUS       DASWBY010000910         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_633440, whole genome shotgun sequence.
ACCESSION   DASWBY010000910 DASWBY010000000
VERSION     DASWBY010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633440"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /gene="dacB"
                     /locus_tag="VF870_16410"
     CDS             <1..1163
                     /gene="dacB"
                     /locus_tag="VF870_16410"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX9253832.1"
                     /translation="GSTLYGDLVIQGRGDPTISGRFYNNDINYVFDTWIDSLLDMGIK
                     NIKGNIVGDDNLFDDIGLGNGWSWDYETDWYAAQSSAISFNDNCVDITIYYDKKYDSV
                     FVSSKPGLRSIVILNNVKPAVGSEKTNIEVVRERGTNVITVSGKFRKDADSLVTYATV
                     QNPTQFAMLVFKNRLESRGIRVNGYAIDIDDYERTINKSDLELLFISYSEKLCEIIKV
                     INKGSQNFFAEQLLKTIGLEKFGLGSVANGVAAVQEVFAEIGLNPENIVMADGSGLSY
                     MNMVTPKQVVELLKYMYTNKKVYTDFYNSLPIAGIDGSLGKRMQNTTAQNNVRAKTGY
                     ISHVRSLSGYALTADNEPIAFSMIANNFNVPVKLAENIQDLVCLRLTNFRRK"
     gene            1166..>2592
                     /gene="lysS"
                     /locus_tag="VF870_16415"
     CDS             1166..>2592
                     /gene="lysS"
                     /locus_tag="VF870_16415"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX9253833.1"
                     /translation="MENNAQLSVNDLIKRRYEELEELTKKGVETFAYSFDVNSDSQKI
                     KSNYKDGVEMNVRIAGRIMAIRRMGKASFAHIQDHNGRIQVYLKKDEIGESYDAFKLM
                     DIGDIIGVEGFVFITKTGEISVHTRELKLLSKSLQPLPIAKEVVDEQGNKVIYDQFAD
                     KELRYRRRYVDLIVNPDIKDVFIKRSKIISAIRNYLDTSGYLEVETPILQPLYGGAAA
                     RPFVTHHNALDTELYLRIADELYLKRLIVGGFNGVYEISKDFRNEGMDRSHNPEFTMM
                     ELYVPYKDYNWMMEYVEKMFEHVCNTVFNTLSFNVDGTEINFKAPWKRVSMIEAIEEK
                     TGLNVLNCSMDDLKSASKKVGIDVGLIHSRAKFIDELFSVKVEPELIQPTFVIDYPLE
                     LSPLAKKHRTKEGVVERFEGYVFGREICNAFSELNDPIDQRNRFEDQGKMKEAGDDEA
                     HVIDEDFIRALEFGMPPTAGLGVGI"
BASE COUNT          912 a          331 c          524 g          825 t
ORIGIN      
        1 cggggtcaac actttatggc gatttagtta ttcagggaag aggtgaccca actatctcag
       61 gaagattcta taataatgat ataaactatg tttttgatac ctggatagat tcattgcttg
      121 atatgggtat taaaaatatt aaaggcaata ttgttggcga tgacaatttg tttgatgata
      181 tcgggttggg aaatggctgg tcatgggatt atgagactga ttggtacgcg gctcaatcaa
      241 gtgcaatatc ttttaatgat aattgtgttg atattactat ctattacgat aaaaaatatg
      301 attctgtttt tgtatcctcg aagccaggtt taagaagcat tgtaattctt aataatgtta
      361 aacctgcagt tggaagtgaa aagactaata ttgaagttgt tcgtgaaaga ggaacaaatg
      421 taattactgt ttcaggcaag tttagaaaag atgctgacag tttagttact tatgctacag
      481 ttcaaaatcc tacgcaattt gctatgttag tttttaagaa ccgattagaa agcaggggaa
      541 tcagagttaa tgggtatgct atcgatatag atgactatga aaggacaatt aataaatccg
      601 atcttgaatt attatttata agttattcag aaaaactttg cgaaattatt aaagtaataa
      661 ataaaggcag tcagaatttt tttgctgaac agttattaaa aactattgga ttagaaaaat
      721 ttggattagg ttcagttgca aacggcgtag ctgcggttca ggaagtgttt gcagaaatag
      781 gattaaatcc tgaaaatatt gttatggctg atggaagtgg tttatcatat atgaacatgg
      841 ttacacccaa gcaggttgtt gaactgttaa aatatatgta caccaataaa aaagtatata
      901 ctgattttta taattcatta ccgattgcag gtattgatgg ctcgctggga aaaagaatgc
      961 aaaacacaac tgcacaaaac aatgttagag ctaaaaccgg ttatattagt cacgttcgca
     1021 gtctatctgg ctatgctctt acagcggata acgaaccaat agcattttca atgattgcaa
     1081 ataattttaa tgtacctgta aaacttgcag aaaatataca agaccttgtc tgtttacgat
     1141 taacaaattt caggagaaaa taattttgga aaacaatgcg caactatctg ttaatgattt
     1201 aattaaaaga cgctacgaag agcttgaaga attaactaag aagggtgtag aaacattcgc
     1261 ttattcattc gatgtaaatt cagattcaca aaagattaaa agcaattata aagatggtgt
     1321 tgaaatgaat gtcaggattg ccggaagaat tatggcaatt cgtagaatgg gaaaagcatc
     1381 attcgcacac attcaggatc ataatggcag gatacaagtt tatcttaaaa aagatgaaat
     1441 tggagaatct tatgatgcat ttaaattaat ggatattggt gacattattg gagtagaagg
     1501 atttgtattt attacaaaaa ctggtgagat ttctgttcac acacgtgagt taaaattatt
     1561 aagcaaatca ctgcagccgc ttccaatagc aaaagaagtt gttgatgaac aaggtaataa
     1621 agttatttat gatcaatttg ctgataagga acttcgatat agaagaagat atgttgatct
     1681 tattgttaat cctgatatta aagatgtatt tataaaaaga agtaaaatta tttctgcaat
     1741 cagaaattat cttgatacca gcggatatct tgaagttgaa actccaatac ttcagcctct
     1801 ttatggaggt gctgcagcac gtccatttgt aacacatcac aacgctctgg atacagaatt
     1861 gtacttaaga attgctgatg aactttatct taaaagatta attgtcggcg gatttaatgg
     1921 tgtttatgaa atatcaaagg attttagaaa tgagggaatg gatcgttcac ataatcctga
     1981 gtttactatg atggaacttt acgttcctta taaagactat aactggatga tggaatatgt
     2041 agaaaaaatg tttgaacatg tttgtaatac tgtgtttaat actttgtctt tcaatgttga
     2101 tggaactgaa attaatttta aagcaccctg gaaaagagtt tcaatgattg aagccattga
     2161 agaaaaaact ggtcttaatg ttctaaattg ctcaatggat gatctgaaat ccgcatcaaa
     2221 aaaagttgga atagatgttg ggttgattca ttcacgtgct aagtttatag atgagttatt
     2281 cagtgttaaa gttgagcctg aattaataca gccaactttt gtaattgatt atccacttga
     2341 attatcacca ctcgctaaaa aacatcgtac aaaagaaggc gtggttgaaa gatttgaagg
     2401 atatgttttt ggcagagaaa tttgtaatgc attttctgaa ttaaatgatc caattgatca
     2461 aagaaacaga tttgaagatc agggaaaaat gaaagaggcc ggagatgatg aagcacatgt
     2521 aattgatgaa gattttattc gtgcacttga atttggtatg ccgccaacag ctggtttagg
     2581 tgtgggcatt ga
//
LOCUS       DASWBY010000911         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_409272, whole genome shotgun sequence.
ACCESSION   DASWBY010000911 DASWBY010000000
VERSION     DASWBY010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409272"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..662)
                     /locus_tag="VF870_16420"
     CDS             complement(213..662)
                     /locus_tag="VF870_16420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEX9253834.1"
                     /translation="MIIDKLSNSNLYSGLGERISKAFTYLKQTDFSKLELGKYEIDGD
                     NIFALVNEYNTKDQSEGKLEAHKKYIDVQFIAKGRELMGYAPLGNQKVIDEYNEQNDI
                     TFFSGAKSFTQVNEGMFAIFFPTDVHLPGIKVNEKSYVKKVVIKVKV"
     gene            complement(779..2035)
                     /locus_tag="VF870_16425"
     CDS             complement(779..2035)
                     /locus_tag="VF870_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253835.1"
                     /translation="MKAVILFLSLFSFLLLTDKTYSQGEAAVPFLLLQPSPSLAAMGQ
                     TGTALPTEDPFGFLWNPAQLGYTSQNNNLSFIFYPSKTEWLPQFNLDLELKGVAFNAG
                     YNFKDIINFPLSVGFGYSNFEINFGEFIRYDEQGNPIGTIHSKDYYDAYSIGVGFDYY
                     VQFNAGLTSKSVTSILSDRPAGEEEAYGSWKGTAYDFGLLINVPGLEIINEEMQIKLE
                     NDMLLKPDFNFSVGYSKSNLGDEVYYIDKNQADPLPRVDRIGYGITTGFDLLAENFQM
                     KLLNFALTVEADDILIDRDSTGWDYQSTLSDLQFVKNLINVEGTEDIVSHIGLKIDLF
                     ETVSFSTGHFSGRGFMDVAETGGYELRAKGIFKLWALWAKGPFTDFLRDHIDIRYYNT
                     NYFADDWRETKMTGLALYVHNINSLF"
BASE COUNT          766 a          427 c          369 g          796 t
ORIGIN      
        1 gttgcaatga tttgtaagtt ctatcattat agatgaagga taggaaactt ttagactttt
       61 tgatttttcg agaagaagtg tttgaatttt attattggaa tatcttaaaa gatttattcc
      121 ttttggtaag tgctgaatgc gttttattgc ttttagtaat tttcttttag tcataactta
      181 aaataaggtt taacgctaaa aacaaaaagc aattaaacct taactttaat cacgactttc
      241 tttacataag atttttcatt caccttaatt cccggtaaat gaacatcggt ggggaaaaag
      301 atagcaaaca ttccctcatt tacttgggtg aatgattttg ctccgctgaa gaatgttata
      361 tcattttgtt cgttgtattc atcaataact ttttggtttc ctaacggagc gtagcccatt
      421 aattctctgc cttttgctat aaactgaaca tcgatatatt ttttatgtgc ttccagcttt
      481 ccttcgcttt gatcttttgt gttgtactca ttaactaaag caaaaatatt gtcaccatca
      541 atttcgtatt tacctaattc tagtttagaa aaatcagttt gttttagata ggtaaaagcc
      601 ttgctaattc tttcaccaag tccggaataa agatttgagt ttgaaagttt atctattatc
      661 atttttatcc ttttacttgt tcgtcattac gaatctcgac ttgtcgggat gaagcaatcc
      721 atattataaa ttgcgacgtc gctatgctcc tcgcaatgac agttaaattt tatttattct
      781 aaaataaaga atttatgtta tgtacgtaaa gagcaagccc agtcattttt gtttcgcgcc
      841 aatcgtcagc aaagtaattt gtgttgtaat atcgtatatc aatatgatca cgtaagaaat
      901 ctgtaaatgg tccttttgcc cataatgccc aaagcttaaa aattcctttt gctcttagtt
      961 catacccccc ggtttctgct acatccataa atccacgacc cgaaaaatgt ccggtgctga
     1021 aagagacagt ttcgaaaaga tcaatcttta atccgatatg tgaaactata tcttcagtgc
     1081 cttcaacgtt tattaaattt tttacaaact gaagatcgct taaagtggat tgataatccc
     1141 aacctgttga gtctctgtca attaaaatat catctgcttc aacagttaaa gcaaagttta
     1201 atagtttcat ctggaaattt tctgcaagta aatcgaatcc ggttgtaata ccgtaaccaa
     1261 ttctatccac tcttggaagc ggatcagcct gatttttatc aatataatat acctcatcac
     1321 ctaaattgga tttagaatat ccgaccgaga aattaaaatc aggtttcaat aacatatcat
     1381 tttctaattt tatttgcatt tcctcattga tgatctccaa acctggaaca tttattagta
     1441 aaccgaaatc atatgctgtc cctttccaac ttccataagc ttcttcttca ccagcaggtc
     1501 gatctgagag aatagatgtt acacttttag atgtcaatcc cgcattaaat tgtacgtaat
     1561 aatcaaatcc aacaccgata gaataagcgt cgtaataatc tttagaatga atcgtaccaa
     1621 tcggatttcc ttgctcatca tatctaataa actcgccaaa gtttatttca aaatttgaat
     1681 atccaaatcc aacacttaag ggaaaattaa ttatgtcttt aaaattatag cctgcattaa
     1741 acgctactcc ttttaattcc aaatctaagt tgaactgtgg aagccattcg gtcttcgaag
     1801 gatagaaaat aaatgaaaga ttattgtttt ggctcgtgta accgagctgc gcgggattcc
     1861 ataaaaatcc aaatgggtct tctgttggta aggcagtgcc tgtctgtccc attgcagcaa
     1921 gtgatggtga aggctgaagt aataagaacg gaacggcggc ttctccctgg gagtatgttt
     1981 tatcagtaag taaaagaaat gaaaaaagac tgagaaaaag aattacagct ttcataactc
     2041 tccctgaata ttgatatcaa aactatataa actgaactaa aataaaacat ataaaaatgt
     2101 ttgcactaat tctctgtcaa cctgaactcg ttttaggccc ttcaaattaa taaagactaa
     2161 aacttagatt ccgaagcaag tctggaatga cattattcgc tgatgctata agttacaaca
     2221 gctgaaagct tataactgaa ctccaattgc aagcttacca attgtttgtt ttctgaaatc
     2281 accggagaaa agatgtgaat cagcaattgc taaatcagcc gagaaaatat catagttaaa
     2341 tcttaagccg ccaataaa
//
LOCUS       DASWBY010000912         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_633596, whole genome shotgun sequence.
ACCESSION   DASWBY010000912 DASWBY010000000
VERSION     DASWBY010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633596"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VF870_16430"
     CDS             <1..276
                     /locus_tag="VF870_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253836.1"
                     /translation="ILTVSSCQKYEIFPSTGYEEVTLKNLTGLDGCGFVFQKVDNKYL
                     EATNMKNFINTYVDGQKYWIKYKIASDAASICMVGDIIEIVRLRDRP"
     gene            744..>1778
                     /locus_tag="VF870_16435"
     CDS             744..>1778
                     /locus_tag="VF870_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253837.1"
                     /translation="MKWPNLILMIIIIIVFSNCERDETDDHPDWIYTAYKFLILDSNK
                     VEYNTRAKFFRLNDSEFFLNGYNNLTQKDCFSKLMFIGNNLEIKNMKSLIYSTPRDIK
                     DISFPNSTIGYLLMNNKEAGTGIDSKLFQTFDGGESWNEKTINLQNTFRWIHFITPDS
                     GIAIRENYTGISYDIYSTTNSGNNWQKIVNEYFVNGRTLSDFYFIPQKPRICFLSSSG
                     QLFYSSNGGFNWKLHSIHNADIVSMSFLNENVGFIVNSKVTYPESLLSTANSIYKINE
                     IGGSYKKVYTGDWDILKIEALNYNEIYINKWYSMYYTSDGFTTVKLMKIQDPLPKVPG
                     DGLVVDFSIYS"
BASE COUNT          617 a          300 c          292 g          569 t
ORIGIN      
        1 atattgactg tatcatcctg tcaaaagtat gagattttcc catcaactgg ttatgaagaa
       61 gtaacactaa aaaatcttac tggattggat ggatgtggct ttgtctttca aaaagttgat
      121 aataaatatc ttgaagcaac aaatatgaaa aattttatca atacttatgt tgatgggcaa
      181 aaatattgga ttaaatataa aattgcctct gatgctgcaa gtatctgcat ggttggagac
      241 attattgaga ttgtaagatt acgcgaccga ccatgataat cttaaaaaac tagctagaac
      301 acggacaata aagttcattt gtttgtcaca ctttttgcat tttgtacctg cacccatgac
      361 agttttgtaa tagcttatac cgcagccgcg ccagtgacct caataaaact ttgtatacct
      421 tgacttgtta cctgctccca atcaaccttg taccgcgaca ctcgtatagc tcagacagat
      481 tgtacctggc ctccttcgcc aaggctacgg aggctaaaag acggcaaaaa gtgcgccatg
      541 gtttgttcat ttcggctgtg tatcatgatt ctaccccgat tagacatttc tactcacagc
      601 ctccattttc aaaccaacac aaacgcactt tattgccggg ccgttaggca acattaatgt
      661 ccctagcaag acgaatataa gagccagaat aatgtttact aatttcgatt aaacttaggt
      721 attgtacaat taaaacaatt gatatgaagt ggccaaattt gattttaatg ataattataa
      781 taattgtctt ttcaaactgt gaaagagacg agactgacga tcatccagac tggatatata
      841 cggcatataa attccttatc ttggatagta ataaagtcga atataataca agagcaaaat
      901 tctttagatt gaatgattct gaattctttt tgaatggata taataatttg actcaaaaag
      961 attgtttctc taaactgatg tttattggta acaatttaga aattaagaat atgaaatcat
     1021 taatatatag cactccaaga gatattaagg atatatcatt ccccaatagt acaattggat
     1081 accttttgat gaataataaa gaagcaggga caggtataga ttcaaaacta tttcaaacat
     1141 ttgacggagg cgaatcatgg aacgaaaaaa ctataaacct ccaaaacaca ttccgctgga
     1201 ttcatttcat tactcctgat tcaggaattg caattagaga aaactacact ggaataagtt
     1261 atgatattta ctctactact aatagtggaa ataattggca aaaaattgta aatgaatatt
     1321 tcgttaatgg tcgaacttta tctgacttct actttattcc acaaaagccc agaatttgct
     1381 ttctttcatc ttcaggtcaa cttttctatt caagtaacgg aggttttaat tggaaattac
     1441 atagtattca taatgcagat attgtctcaa tgtctttcct aaatgaaaat gtaggcttca
     1501 ttgtcaatag caaagtgact tatcctgaat ctttacttag tactgccaat agtatttata
     1561 aaataaatga aattggggga tcttataaaa aagtatatac aggagattgg gatatcttga
     1621 aaattgaagc acttaattat aatgaaattt atatcaataa atggtattca atgtattaca
     1681 cctctgatgg tttcactact gttaagctaa tgaaaattca agatcccctt ccaaaagttc
     1741 caggagacgg acttgtcgta gatttctcaa tatattca
//
LOCUS       DASWBY010000913         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_409431, whole genome shotgun sequence.
ACCESSION   DASWBY010000913 DASWBY010000000
VERSION     DASWBY010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409431"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /gene="nosZ"
                     /locus_tag="VF870_16440"
     CDS             complement(<1..1566)
                     /gene="nosZ"
                     /locus_tag="VF870_16440"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HEX9253838.1"
                     /translation="MKNISRKTLLSAFGILFLIALTYAGCNQSKTSLSGNEAQQVYVA
                     PGTYDEFYLFTSGGFSGQMGVYGLPSGRHFKTISVFAQNPETGYGYSEETKAMLNTTY
                     GFIPWDDSHHPEFSMTDGVPDGRFIFINGNNTPRVAKIDLKEFETTEIIEIPNSAGNH
                     ASPFVTENTEYIIASTRFSIPIPQSDVSISSYKENFKGTISFIKLDPKTEKMNLEFQI
                     IVPGFNYDLAHAGKGPSHDWAFFTSYNSEQANSLLEINASKNDKDFVAAVNWKKAEQY
                     LKEGKAKTYDAEYYRNYVDHKTQIAVSEINDQVKVLDPKDCPGMMYYLPTPKSPHGVD
                     VDPTGEYIVAGGKLASVIPVHSFTKMMKAIEEKQFDGEIAGIPILKYDAVVAGEVKDP
                     GLGPLHTEFDDNGYAYTSAFISSEIVKWKIGTWEVVDRIPTYYSIGHLCIPGGDSKKP
                     YGKYVIALNKITKDRYLPTGPELTQSAQLIDITGEKMKLLLDFPTVGEPHYAQAIPSD
                     LIMKNSVKFYKIEE"
     gene            complement(1587..>2010)
                     /locus_tag="VF870_16445"
     CDS             complement(1587..>2010)
                     /locus_tag="VF870_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9253839.1"
                     /translation="CGDNAPQDPSKFSNTKKVENAGGLSGFELENGIGPIKQKIELKA
                     IDPILVKQGQGIFETKCAACHKLDERYVGPAQRDVFKRRSPEYILNFMLNPEENYKRH
                     PEAKKLLGEYMTQMPNQNLTIDEAKAMLDYFRKIADEK"
BASE COUNT          562 a          380 c          349 g          719 t
ORIGIN      
        1 ttcttcgatc ttgtaaaact taactgagtt tttcataatt aagtctgaag gaattgcttg
       61 tgcataatgc ggctcaccaa cggttggaaa atcaagaagc agtttcatct tttctcccgt
      121 aatatctata agctgtgctg attgagttaa ctcaggtcct gttggaagat atctgtcttt
      181 tgtaatttta ttcaacgcga taacatattt gccataaggc tttttactat cgccaccagg
      241 aatacacaag tgtcctatcg aatagtaggt tggaattcta tcaacgactt cccaggtacc
      301 aatcttccat tttactattt cagaagagat gaatgctgag gtgtatgcat atccgttgtc
      361 atcaaactct gtatgtaatg gtccaagacc tgggtcttta acctcgccgg caactactgc
      421 atcatatttt aatattggta tgccagcaat ttcaccatca aattgttttt cttcaatagc
      481 tttcatcatt ttggtgaatg aatgaacagg aattactgat gcaagttttc caccggcaac
      541 aatgtattca ccagtcggat caacatcaac gccgtgtggt gattttggtg ttggcagata
      601 atacatcata ccggggcaat ctttaggatc aagaactttt acctgatcat ttatttctga
      661 aacagctatt tgggttttat gatcaacata atttctataa tactcggcat cgtatgtttt
      721 agctttacct tcttttagat attgctcagc ttttttccag ttaaccgctg caacgaaatc
      781 tttatcattt tttgaagcat taatttctag aagtgaattt gcctgttcgc tattgtagct
      841 agtaaaaaaa gcccaatcgt gtgatggacc cttaccagcg tgtgcaagat cataattaaa
      901 tccgggtaca attatctgaa attccagatt catcttttcc gtctttggat caagtttaat
      961 aaaactgatt gttcccttaa aattttcttt gtaggacgaa attgagacat cactttgagg
     1021 aattggaata ctaaatctag ttgaggctat aatgtattct gtgttttcag taacgaatgg
     1081 tgaagcgtgg ttgcccgcag aatttggtat ttcaataatt tctgttgttt caaattcctt
     1141 taaatcaatc tttgcaacac gtggagtatt atttccgtta ataaaaataa atcttccgtc
     1201 gggtacaccg tctgtcatag aaaattctgg atgatgtgaa tcatcccatg ggatgaatcc
     1261 atacgttgta ttaagcatag cttttgtctc ttcagaatat ccataacctg tttctggatt
     1321 ttgtgcaaac acagaaattg ttttaaagtg cctgccagaa ggtaatccat atacacccat
     1381 ctgtccgctg aatcctcctg aagtaaaaag ataaaattca tcataagtac ctggtgctac
     1441 ataaacttgt tgtgcctcat ttcctgaaag tgaagttttt gattgattgc atccagcata
     1501 ggttagtgca attaaaaata gaattccaaa agcactcaac aaagtttttc ttgaaatgtt
     1561 tttcataagc tgcctttttt tattggttat ttttcgtctg ctatttttcg gaaataatca
     1621 agcatcgcct ttgcttcatc tattgtaaga ttttgatttg gcatctgagt catatattcg
     1681 cctaataatt tttttgcttc cggatgtcgt ttataatttt cctcagggtt aagcataaag
     1741 ttcaagatgt attccggact tcttctttta aaaacatcac gctgagctgg gccaacatac
     1801 ctttcatcaa gtttatggca tgctgcacat ttagtctcaa atattccctg accttgttta
     1861 acaagtatcg gatcaatagc ttttaattct attttttgct tgattggtcc aataccattt
     1921 tctaattcaa agcccgaaag accacctgca ttttcaactt tttttgtatt gctaaatttt
     1981 gatgggtcct gaggagcatt atcgccgcag
//
LOCUS       DASWBY010000914         5408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_336481, whole genome shotgun sequence.
ACCESSION   DASWBY010000914 DASWBY010000000
VERSION     DASWBY010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336481"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VF870_16450"
     CDS             <1..1338
                     /locus_tag="VF870_16450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9253840.1"
                     /translation="ANSTNTDIYLMPLGGTQNVNITLANDAWDGAPTFSPDGNYIAYR
                     TQLIPGFEADRFRVAVFNTKTLQSEVLTEDFDYTTNDLSWSADSKSIYFVADYKGYSP
                     VYKVDLDTKKVVQVTDDKAVRGYQVSKDDQVVYLTFSTVDKPAEMFSYTVNSNAYAQI
                     TYFNKKLTEEVDIRPAEQMWVEGADGVPVHVFIVKPHNFDEYKRYPLIINVHGGPQMQ
                     WMDSFRGDWQVYPGSGYVVAFFNPHGSTGYGSKYTEAISKDWGGKVYEDVMKVTDALE
                     KLRYVDSDRMGAMGWSFGGYMMNWLQGQTKKFKCLASMMGIFDLESMWGSTEELWFAN
                     WDLGGQPLNSELYKKYSPSNYVQNFSTPTLIITGEKDYRVPYTQSIQYFTTLRSLGID
                     SRLIIFKNDGHWPNNVKSMPLYYNAHLEWFHKYLDGDPAPYDSKELVKNNVFK"
     gene            1537..1986
                     /locus_tag="VF870_16455"
     CDS             1537..1986
                     /locus_tag="VF870_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253841.1"
                     /translation="MVRNILGVVLGYVAIFIFVFITFTVLYFILGADGSFEPGTYEVS
                     FIWIVLSFILGLVAAVLGGYICVLISKNQKTALVLAGFVLVLGIVMAIPALGDTTNEV
                     QEMRSADVPNMEAMQKAKQPAIMLLLNPIIGALGVFAGSKLRKEETV"
     gene            complement(2048..2299)
                     /locus_tag="VF870_16460"
     CDS             complement(2048..2299)
                     /locus_tag="VF870_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9253842.1"
                     /translation="MSTKLFVGSLPWSVDDEALREAFEGHGSVVSAKVVKDRDTGRSR
                     GFGFVEMENSSEASNAMKALNESELKGRNIVVNEAKSRS"
     gene            2543..2941
                     /locus_tag="VF870_16465"
     CDS             2543..2941
                     /locus_tag="VF870_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX9253843.1"
                     /translation="MNIDITTPALLFPAISLLLLAYTNRFLTLATIIRSLHQNYKEKP
                     NHVLLGQIENMRKRVHLIKHMQGVGVGSLFLCVLCMFLLFAGQYELGKYIFGLSLVML
                     LISLILSLREIAISVDALELHLSDIEKKGS"
     gene            complement(3018..3350)
                     /locus_tag="VF870_16470"
     CDS             complement(3018..3350)
                     /locus_tag="VF870_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9253844.1"
                     /translation="MNIFVGNLSNDVTEEDLNNLFSEFGQVKEVKIIRDMFSQQTKGF
                     GFVEMPGLADAQKAINGLNTKEVKGKKLVVNEARPKTDSRKRFNGGGGGGGRSGGSGG
                     GQNNRRRY"
     gene            complement(3598..>5408)
                     /locus_tag="VF870_16475"
     CDS             complement(3598..>5408)
                     /locus_tag="VF870_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX9253845.1"
                     /translation="LIEFAETIKKKDKSSIGEEKKEDWRNVPVEERLKHALVKGIVDF
                     IDVDIEEARKRYPQPIEVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKKAVAIL
                     IPFIEAEKEKNKRTREQGKVLMATVKGDVHDIGKNIVGVVLGCNNYNVIDLGVMVHTE
                     KILQTAVDEKVDVIGLSGLITPSLDEMVHVAKEMERRGMKLPLLIGGATTSRIHTAVK
                     IAPNYSGAVIHVLDASRSVPVVSNLLNENEEERKKYVQSFKDEYAQAREDYSKRKSDK
                     KYISLDKARENRLKIDWQKTEIKKPNKLGITILKEFNLETLRSYIDWTPFFMTWELKG
                     KYPAIFDDKNVGPEAKKLFDDANKLLDKIISEKLLTANGVIGLFPANSVGCDDIEIYA
                     DESRSGVKRVLHTIRQQIEKSKDEPNIALADFIAPKETGVEDYIGMFAVTAGGGIENI
                     VAQFEKDHDEYNSIMVKAIADRLAEAFAEHLHKLVRKEYWGYAADENYSNEELIKEKY
                     IGIRPAPGYPAQPDHTEKPIIFSLLDVEKNAGIKLTESMAMYPAASVSGLYFSHPEAK
                     YFNVGKIEKDQVLDYHRRKGISVEELEKWLSPILNY"
BASE COUNT         1597 a         1019 c          930 g         1862 t
ORIGIN      
        1 gcaaactcca ctaataccga catttattta atgcctttag gaggcacgca aaacgtaaat
       61 ataactcttg caaatgacgc gtgggatggt gcaccaacat tttcaccaga cggaaactat
      121 attgcatatc gaactcagtt aatacccgga tttgaagcgg acagattccg tgttgctgta
      181 ttcaacacaa aaactttgca atccgaagta ttaacagaag attttgatta caccactaat
      241 gatctttctt ggtccgcgga ttccaaatca atttattttg tagcagatta taaaggttat
      301 agcccggttt ataaggtcga tctggataca aaaaaagttg tacaggttac tgatgataaa
      361 gcggtgcgtg gatatcaggt aagcaaggat gatcaggtgg tctatttaac tttttctact
      421 gttgataagc cagctgaaat gttttcatat actgtaaatt ctaatgctta tgcccagata
      481 acatatttca acaaaaagtt aacagaagaa gttgacattc gtccggctga acaaatgtgg
      541 gttgaaggag ctgatggtgt tccggttcac gtttttattg ttaagccgca taattttgat
      601 gaatataaaa gatatccatt aataataaat gtacatggtg gtcctcaaat gcaatggatg
      661 gattccttca gaggtgattg gcaggtttat ccgggctctg gttatgttgt tgcgtttttt
      721 aatccacatg gatcaaccgg ttacggaagt aaatacacag aagctatttc aaaagattgg
      781 ggtggaaaag tttatgaaga tgttatgaaa gtaaccgatg cgctggaaaa attacgttac
      841 gtagatagcg atagaatggg cgcaatggga tggtctttcg gcggatatat gatgaactgg
      901 ttgcaggggc aaacaaaaaa atttaagtgc ctggcttcaa tgatgggaat atttgatctt
      961 gaatctatgt ggggatctac tgaggaatta tggttcgcaa attgggattt gggtggacag
     1021 ccgttgaatt ctgagcttta taaaaaatat tcgccatcta attatgtaca gaatttttct
     1081 actccaactt taataatcac tggtgaaaag gattatcgtg ttccttatac acaaagcata
     1141 caatacttta ctacattacg atcattaggc atagacagcc gtttaataat attcaagaac
     1201 gatgggcatt ggccgaacaa tgttaaatca atgcctttat attacaatgc acatttagag
     1261 tggttccata aatatcttga cggtgaccct gcgccgtacg attctaaaga gttagttaag
     1321 aacaacgttt ttaaataaaa attaatatta agaggcgtat tatttattac gcctctcttt
     1381 ttttatcata acctcaattt ctcattaaca ttattgattt ataaaatcta caattttgtt
     1441 aaccaaacag ttgcctcttg ttaaataaaa aattagtttc gctttcaaac ctggagaaaa
     1501 atcaacccaa acaataaaat tatttaggag gctttcatgg ttcgcaatat acttggtgta
     1561 gttttggggt atgtggcgat atttattttt gtgttcatca cctttactgt tctttatttt
     1621 attcttggtg cggacggatc ttttgaaccg ggtacatatg aagtttcgtt tatttggatt
     1681 gtattgagtt ttatacttgg tttagtcgca gctgttttgg gcggatatat ctgtgtttta
     1741 atttccaaaa atcaaaagac agctttggtg ttagctggat ttgttttagt gctcggcatt
     1801 gtaatggcaa ttcctgcatt gggtgataca acaaatgaag ttcaagaaat gagaagtgcc
     1861 gatgttccaa acatggaagc aatgcagaaa gctaaacagc cggcaattat gttattatta
     1921 aatcccataa ttggcgcact tggagttttt gctggatcga agttgagaaa agaggaaacc
     1981 gtataagtct ttaaaattaa aaaggcgtcc ataggacgcc tcaagtaagt caaatcagaa
     2041 actgttatta gctgcgtgat tttgcttcat taactacaat gtttcttcct tttaattccg
     2101 actcatttaa tgctttcata gcattacttg cttcggagga gttctccatt tcaacgaaac
     2161 cgaatcctct ggatctgccg gtatcgcggt ctttaactac ttttgctgaa acaacacttc
     2221 cgtgtccttc aaatgcttct cttaatgctt cgtcgtcaac cgaccatggg agagaaccca
     2281 caaataactt agtactcact agaacctcta aaaataaata gtaaaaaaat tccggcctat
     2341 taccggaggg ccaaaaataa aactaatacc gagaataacc taattaattc aagcatattg
     2401 tatcttggta agataatctg tgattttaat ttttatcaaa gccttaatta ttgggataat
     2461 taacataatt gattctttgt gttaattata tatataacta ataatctata cttttgtgta
     2521 ttaattttaa aaggctttta taatgaatat agatatcaca acgccagcat tattatttcc
     2581 cgcaatttca ttattacttc ttgcatatac taacagattt ttaacccttg ccactattat
     2641 tcgatcttta catcagaatt ataaagaaaa acctaatcat gttttactgg ggcaaattga
     2701 aaatatgcgc aaacgagttc atcttataaa acatatgcag ggtgtgggag ttggcagttt
     2761 gtttctttgc gtgttatgta tgtttctttt gtttgcggga cagtatgaat tgggaaaata
     2821 tatttttggt ttaagccttg taatgctttt aatttctctg atactatcgc tcagagaaat
     2881 tgccatatca gtagatgcac ttgaattaca cttaagtgat attgaaaaga agggttctta
     2941 actttttatt acgaacttgg aaaaacaacg ccggtttgtt ctttattaca aagtacaaat
     3001 cggcgtttaa taaataatta atatctgcgt ctgttgttct gaccgccgcc gctgccgccg
     3061 gatcttccac caccgccacc gccgccatta aagcgttttc tgctgtcggt tttagggcga
     3121 gcttcattaa caacaagttt ctttccctta acttctttag tgtttaaacc gttaatcgct
     3181 ttttgcgcat ctgcaagtcc tggcatttct acaaaaccaa atcctttagt ttgctgcgaa
     3241 aacatatcgc gaataatttt aacttcttta acctgtccga actctgaaaa aagattattt
     3301 aaatcttctt ccgtaacatc gtttgacaaa ttgccaacga agatattcat agtaactcct
     3361 gataattata aaaaaatatt aataacgaga gagcatattg tttatgcggc tcttcttcct
     3421 gtggattaaa aatagaacga ctatttgtgg tatgacttca aaattttaat caggcatttg
     3481 aaagatagtt taattaattc tgccaaacaa cataactcgc ggaacttatt aacatcaatt
     3541 acagaacatg aaagttatcc tggcttccga ttcattgaaa tattgattaa aacgatatta
     3601 ataattcaat ataggactta accacttttc aagttcttca acactaattc ctttgcggcg
     3661 atgataatca agtacctgat ccttttcaat ttttcccaca ttaaaatatt ttgcttcagg
     3721 atgggagaaa tacaatccgc ttacacttgc tgcgggatac atagccatgc tttcggttag
     3781 tttaattccc gcattctttt caacatcaag caatgaaaaa ataatcggtt tttctgtgtg
     3841 atcaggctga gccggatagc ctggcgcagg acgaatacca atatattttt cttttattaa
     3901 ctcttcattt gaataatttt catccgctgc atagccccaa tattcttttc tcacaagctt
     3961 gtgaagatgt tccgcaaaag cttctgctaa tctatctgca atcgctttta ccattatgct
     4021 gttgtattcg tcatgatctt tttcaaattg tgccactata ttttctattc ctccgccggc
     4081 agttaccgca aacattccta tgtaatcctc aactccggtt tcctttggtg cgataaaatc
     4141 tgcaagtgca atgtttggtt cgtctttaga tttttcgatt tgctgtcgta ttgtatgcaa
     4201 aactcttttt actccgctcc gcgattcgtc tgcatatatt tctatgtcat cacagccaac
     4261 agaatttgca ggaaataatc caatcactcc atttgcggta agtaattttt ctgaaattat
     4321 tttatcaaga agtttatttg catcatcaaa aagtttcttg gcttctggtc caacgttctt
     4381 gtcgtcaaat attgctggat acttgccttt taattcccac gtcataaaga atggagtcca
     4441 atcaatataa ctgcggagtg tttccagatt aaattcttta agaatagtta ttcctaattt
     4501 atttggtttt ttaatttctg ttttttgcca atcaattttt aatctattct ctcttgcttt
     4561 atccaacgag atatatttct tatcagattt ccgttttgaa taatcctctc ttgcctgagc
     4621 atactcgtct ttaaacgact gtacatattt ttttctttct tcctcatttt catttaaaag
     4681 gtttgataca accggaacgc tgcgagacgc atcaagaaca tgaataactg caccactata
     4741 gttaggtgca attttaaccg cggtgtgaat gcgtgaagtg gtggcgccgc caataagtaa
     4801 aggcaatttc attccgcgtc tttccatttc tttggcaacg tgtaccattt catcaagcga
     4861 gggagttata agtccgctta agccaataac atcaactttt tcatctacag ctgtctgaag
     4921 aattttttct gtatgaacca ttactccaag atcaataaca ttatagttgt tacacccaag
     4981 tacaacgccg actatgtttt ttccaatgtc atgcacatca cccttaactg ttgccattaa
     5041 aaccttgcct tgttcgcgtg tccttttatt tttttctttc tcagcttcaa tgaacggaat
     5101 taatattgcc acggccttct tcataacgcg tgcacttttt acaacctgag gcaaaaacat
     5161 tttgcccgcc ccaaatagat ctccaaccac attcattcct gccattagag gaccttctat
     5221 aacttctatt ggctgcggat atctttttct agcttcttca atgtctacat caataaagtc
     5281 aactatgcct ttaaccagag catgttttag cctttcttca accggaacat ttctccaatc
     5341 ttcttttttc tcttcaccaa tggaagattt atctttcttt tttattgtct ctgcaaattc
     5401 aattaacc
//
LOCUS       DASWBY010000915         9019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_676784, whole genome shotgun sequence.
ACCESSION   DASWBY010000915 DASWBY010000000
VERSION     DASWBY010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676784"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..2396)
                     /locus_tag="VF870_16480"
     CDS             complement(186..2396)
                     /locus_tag="VF870_16480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9253846.1"
                     /translation="MKFIIYSFFVLIISMSTTAQSLKGNIYELDDKGEKVPLIGTNVF
                     WEGSQVGTTTDENGFFDLKKIDSDHLYLIVSYIGYEPVDLEIPEDQDTVEIILSINRE
                     LKEVVVTGTSLSKFIDELDAKQTEVITSKELLKAACCNLSESFTTNASVDVQFQDAVT
                     GAKQIQLLGLSGLYTQMLLENIPTLKGLGSTFGLGYVPGPWMTSISVSKGAASVVNGY
                     ESVTGQINLEYKKPDDLERYYFNAFQSSHFKTDLNANAATHLNDNLSTMILAHIDFNQ
                     KAMDENGDTFKDQPNVKQFNFINRWKYQSFKGFESQFGVQVLNEERRGGQISDVHSNS
                     VVDKPYQINVDTKRYEVFAKNGYVFDDDAYKSIGLILNAQWHDQKSLVGLRQYNGNQK
                     TFYANLLFENKSEDDVHALTLGGSYAYDNYNESIDGKESSRNESRPGIFAEYNYSPSN
                     VLSIVPGARVDFHNLYGTFFTPRLHVKYNIDENTTLRASAGKGFRSVNLFAENLNYLA
                     SSRDFVIINNPTYEEAWNYGLNLTRYIPIKDRDLRISAEYYRTDFLKQTIVDIDSDIR
                     EVRFYDLIGKSFSNNYQIEIAYQPIERLDVSAAVRYTDVKANYGDELLIKPLSSKYKG
                     LLTISYSTEQSGWLFDSSFLLNGGGRIPGTKENPIEYQREDSFPAYLTVNAQITKKIE
                     MLDIYLGVENLTNYKQDNPIIASDDPFGEYFDASMIWGPIDGTKFYLGVRLSVL"
     gene            complement(2517..3029)
                     /locus_tag="VF870_16485"
     CDS             complement(2517..3029)
                     /locus_tag="VF870_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253847.1"
                     /translation="MKILVKNILTTIVASALLIGSTGFQVYKHTCSTHNISGVSLIEI
                     PVCEKDNQSIEETADCCKPVIEEPTEDSCCETETSERVNQVSLAPQDINCCISTIEKS
                     QLQDNLFSPVQKKSLITEIANDLVPLFFSIKQNAEQNLVLRNNDLPPPIFGKTLLCTI
                     HQLKIDTPTC"
     gene            3299..4198
                     /locus_tag="VF870_16490"
     CDS             3299..4198
                     /locus_tag="VF870_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9253848.1"
                     /translation="MSHNHNHLSTSNNYNKAFLAGIILNVLYILVEVSYGLLINSMAL
                     LADAGHNLSDVLGLLLAWGAAYLAKTASNEKRTYGLRKSTILAALFNAILLMIAVGAI
                     SIEAIRKFISPEPVQGLTMMVVAGFGVLINALTAVFFMKGRDKDLNIKGAFLHMAADA
                     GVSLGVVIAGFLIYSTGWLWVDPAISLLIILVITIGTWGLLKDSFYLSMDAVPKDIDF
                     QEVENYLNGINGVTEVHDLHIWAMSTTETALTAHLVIPDETKDDYFLKKICDDLHSKF
                     GIEHSTIQTEKSAQSSNCKNNYV"
     gene            4376..5020
                     /locus_tag="VF870_16495"
     CDS             4376..5020
                     /locus_tag="VF870_16495"
                     /inference="COORDINATES: protein motif:HMM:NF039641.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HEX9253849.1"
                     /translation="MNFPFTTEQFLDVFKVYNLTVWPAQIFLLILALMAIFLAIYRTR
                     YSDAVIALTLAFYWLWIGIVYHLLFFTKINTAAYGFAALFVIQSILFFYLGFIKHSLQ
                     FKYSHNTNGIIAIVLFLYALIFYPLLGYYFGHYYPATPTFGLPCPTTIFTLGMLLLLE
                     GKIRKGLFIIPVLWSIIGFTAAIKLGILQDIGLLVAAVLTVAVLVFGRTNKRDA"
     gene            complement(5097..6596)
                     /gene="nrfA"
                     /locus_tag="VF870_16500"
     CDS             complement(5097..6596)
                     /gene="nrfA"
                     /locus_tag="VF870_16500"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HEX9253850.1"
                     /translation="MKPISELTKNKPWLGWLLFFATAVIVFLVGLFASSIIERRSEAV
                     TLQMVKPINDWEPRNEVWGENYPREFESYLKTLDTTYASKHGGSVKRDYLEEYPELVV
                     MWAGYAFSKEYNQGRGHGHAIEDIRNILRTGDNENSPMPATCWTCKSTDVPRVMNEIG
                     ASNFYKKKWIDLGAEIVNPIGCQDCHDPKTMNLRITRPALIEAFKRQGKDMSSFSRQE
                     MRSLVCAQCHVEYYFKGKEEKYLTFPWDRGFSADSMEAYYDAMEFSDWTHALSRAPML
                     KAQHPDYELFKTGIHAIRGVSCSDCHMPYKSEGGVKFTDHHIQSPLNNIANTCQVCHR
                     EETTRLMQDVYDRQDRIEEIRRIAEKTLAAAHIEAKVAWDNGATEEQMKPILKLIRHA
                     QWRWDWVAAANALGFHAPVEALRVLGTSIQKGEQARREIAILFVKQGWKYPVDLPDIS
                     TKEKAQKYIGLDMQKLNAAKQEFLRTTTVKWDEEAKKRQGFLYKYKIKE"
     gene            complement(6620..7219)
                     /gene="nrfH"
                     /locus_tag="VF870_16505"
     CDS             complement(6620..7219)
                     /gene="nrfH"
                     /locus_tag="VF870_16505"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HEX9253851.1"
                     /translation="MKILNIIHSFIPPPQWRIAIIFSLGVLGGLLFLVLHLAKATSYL
                     SDKPETCVNCHVMAPQYATWQNGSHGRVTVCNDCHVPQDNFIKKYFFKASDGLRHSFM
                     FTFRLEPQVIQIKQAGKNAVQQNCIRCHSNVIHPISLRAISNQKIQEEGDGYCWDCHR
                     EVPHGRVNSLSSTPYARIPGLSDPIPEWIKKSLELNKDN"
     gene            complement(7453..8574)
                     /locus_tag="VF870_16510"
     CDS             complement(7453..8574)
                     /locus_tag="VF870_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9253852.1"
                     /translation="MTTLSEIKKKKKIIRNDEKGIQKTRFIVQSLFAALCVWIGIEFY
                     LFNQYLETNGATAFHSRPPGVDGFLPISSFMSFYLFITTGEIHSAHPAGLFIFLSIVL
                     MSLVFGKSFCSWFCPIGYISELIGDFGEKIFKRRLKLPKWLDYSLRSLKYLLLGFLVY
                     SVVFLMSNLALKAFLDSPYNLVSDVKMYYFFANISRAALIVIAALFLLSIFIRSFWCR
                     FLCPYGALLGIMSLLSPNKIQRIAKSCIDCGLCNKACPSFIKVDSVKTVISDECSTCL
                     NCVDACPVKDTLELKSLFPIKKTINKKFVAIGVVAIFMIITGFAMLTGNWQNKITKEE
                     YLMHYKYMNSYGHPTGVKEFKKLNEDVEIQNQQKKLNNK"
     gene            complement(8592..>9019)
                     /locus_tag="VF870_16515"
     CDS             complement(8592..>9019)
                     /locus_tag="VF870_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9253853.1"
                     /translation="VFFSKKCEYGLQAVLFMAASENNIVYPAEEISRKLKIPKEFISK
                     ILQSLTESGIIESKKGKSGGFKLAKHPSKIKLIDIVDAIDGLESFNSCVLGFPKCSPD
                     KPCPVHDQWGSLRTKAYEMLSSETLDKFKEKTLNKINNV"
BASE COUNT         2857 a         1659 c         1381 g         3122 t
ORIGIN      
        1 tgtcttgtgc aaaactcaac acacttaaac agaatccaac tgcaattact aacacgaatt
       61 gttttaacat tttttactcc tttttatttt ttacaattgt cacattgagc gaagtcgaaa
      121 tgtgacattt ttaattcaat aggaattgtc atccttcgac tccgctcagg atgacaattg
      181 atttcttaca atacacttaa tctaacacct aaataaaact tagtaccatc aatcggaccc
      241 cagatcattg atgcatcaaa atattcacca aaaggatcat cggatgcaat tatcggatta
      301 tcctgtttat agtttgtaag attctcaact ccaagataaa tatcaagcat ctcaattttt
      361 ttagttatct gtgcattcac agttaagtaa gcaggaaaac tatcttccct ttgatactca
      421 ataggatttt cttttgtacc tggaattcgt ccaccgccat ttaacaaaaa tgatgaatca
      481 aacaaccagc cgctttgctc agttgaataa gatatagtca gcaatccttt gtacttactc
      541 gaaagcggtt ttattaaaag ttcatcaccg taatttgctt ttacatcagt atatctaaca
      601 gcagcggaaa catcaagtct ttcaataggc tggtaagcta tttctatctg ataattattt
      661 gagaatgatt ttcctattaa atcgtaaaat ctaacttctc tgatatccga atcaatatca
      721 acaatcgttt gctttagaaa atcagttcga taatactctg cagaaattct taaatcacga
      781 tctttaatag gaatatatct cgtgagattc aatccgtaat tccatgcttc ttcataggtt
      841 ggattgttta taattacaaa atctcttgag cttgcaaggt aattaagatt ctcggcaaat
      901 aaatttactg atctgaatcc ttttccggca gatgctctga gcgttgtgtt ctcatcaata
      961 ttatacttta catgcaacct tggcgtaaaa aatgttccat ataaattatg aaaatccacc
     1021 ctggcccccg gaacgattga taatacattt gatggcgagt aattgtattc agcaaatatt
     1081 cctggtcgtg attcatttct gctagattct tttccatcaa ttgattcatt ataattatca
     1141 taagcataac ttccaccaag tgttaatgca tgaacatcat cttccgattt attttcaaaa
     1201 agaagattcg cataaaatgt tttttgattt ccgttatact gccttaatcc aaccaaggat
     1261 ttttgatcat gccactgagc atttagaatt aaaccaatac ttttgtacgc gtcatcatca
     1321 aatacataac cattttttgc aaacacttca tatcttttag tatcaacatt tatttgataa
     1381 ggtttatcaa cgacagaatt cgagtgcaca tcacttattt gtccgcctct tctttcttca
     1441 ttcaatactt gaacgccaaa ttgtgattca aatcctttaa atgattggta cttccatctg
     1501 tttatgaaat taaactgctt aacattcggc tgatctttaa aagtatctcc attttcatcc
     1561 atagcttttt gattaaaatc gatgtgagct aaaatcattg tgcttaaatt atcatttaaa
     1621 tgagtcgctg cattagcatt caaatcagtt ttaaagtgcg aactttgaaa agcattaaag
     1681 taataccttt caagatcatc gggtttttta tattcaagat ttatttgccc tgttactgat
     1741 tcatatccat taacaacaga cgctgcacct tttgatacac tgattgatgt catccatgga
     1801 cccggaacat aacccagacc aaaagtgcta cccaagcctt ttagagttgg aatattttcg
     1861 aggagcattt gagtatataa acctgataaa ccaagcaatt ggatttgttt ggcacctgtt
     1921 accgcatcct gaaactgaac atcaaccgaa gcatttgttg taaagctttc agataaattg
     1981 caacaagcag cctttaacaa ctcttttgat gtgattactt ctgtctgctt tgcatcaagt
     2041 tcatcaataa actttgaaag agaggtaccc gttacaacaa cttccttcag ttcacgattt
     2101 attgataaaa taatttcaac tgtatcctga tcttcaggaa tttctaaatc aacaggttca
     2161 tatccaatgt agcttactat cagataaaga tgatctgaat caattttctt taaatcgaaa
     2221 aaaccatttt catccgtcgt agttccaacc tggctacctt cccaaaatac attagttcca
     2281 ataagtggaa ccttttctcc tttgtcatca agttcgtaga tatttccctt taaactctga
     2341 gcggttgtgc tcattgatat tatcagaaca aaaaaagaat aaattataaa tttcatttta
     2401 caccttattg ttatacagtt gaattatgca gtcattcata gcaagttatc gaacggacga
     2461 atccaatact gccatggatt ccttactaaa tttggaatga catctctaca agaattctaa
     2521 caggtaggtg tatctatttt aagttgatga attgtacaaa gaagtgtttt gccaaatatt
     2581 ggcggaggca gatcgttatt gcgtaaaact aagttctgtt ctgcattttg ttttatacta
     2641 aagaaaagtg gaactaaatc attcgctatc tctgtaatca aagatttctt ttgaacaggt
     2701 gaaaataaat tatcctgtaa ttggcttttt tcaattgtcg atatacagca attgatatcc
     2761 tgcggagcta atgaaacttg attaactcgc tcactggttt cagtttcaca gcaactgtct
     2821 tcagtaggtt cctcaattac cggcttacag caatcagcgg tttcttcaat tgattgatta
     2881 tccttttcac agacagggat ttcaatcaag gatacaccgg aaatattatg agtcgaacaa
     2941 gtatgcttat aaacctgaaa tccggttgac ccaattagta gtgccgatgc aactattgtt
     3001 gtaaggatat ttttaactaa tattttcaat tgctaattat ataaagttga tttatttctg
     3061 taattaaaat aaaactaata gctttaatct caaatggaat ttttaatcac tttcgcaaaa
     3121 atcacttaaa aaatacatca aagcgttggg ttagttttat aactattaaa aatctcaaaa
     3181 gtggagtgaa aaattgaatt ccaccttatt tagataatga gaaaacaatt actggaattt
     3241 tgttagtgat tttagaaata gctggttatt ttttagcata aaatttttga tattaaaaat
     3301 gtctcataac cataatcatt tatcaacatc aaataattat aataaagcat ttctcgccgg
     3361 cataatacta aatgtactat atattttagt tgaagtttcc tacggacttc ttattaattc
     3421 tatggcattg cttgcagatg ccgggcacaa cttaagtgat gtacttggat tattacttgc
     3481 ctggggtgct gcatatcttg caaaaactgc ctctaatgaa aaacgaacat atggtttaag
     3541 aaaatcaaca atactagcag cgttatttaa tgcaattctc ttaatgattg cagtcggagc
     3601 aatatcaatt gaagcaatta gaaaattcat ttcgcctgaa cctgtgcagg gattaacaat
     3661 gatggttgtt gcaggttttg gagttttaat aaatgctctt accgcagtat tttttatgaa
     3721 gggaagagac aaagatctta atattaaagg agcttttttg catatggcgg cggatgctgg
     3781 cgtatcgctt ggagttgtga ttgccggatt tctcatatat tcaaccggct ggctttgggt
     3841 tgatcctgct ataagtctgc tgattattct agtaattacc ataggaacct ggggattgct
     3901 aaaagattca ttttatcttt caatggatgc cgttccaaag gatattgatt tccaggaagt
     3961 tgaaaattac ctgaacggaa ttaatggagt tactgaagta cacgatttac acatctgggc
     4021 aatgagtaca actgaaactg ccctcacagc tcatcttgta atacctgatg aaacgaagga
     4081 tgattatttt ttaaaaaaaa tatgtgatga tctgcattca aaatttggga tagaacattc
     4141 tacaattcaa acagaaaaat ctgcacaaag ttcaaattgt aaaaataatt atgtttaatt
     4201 ttttttgatt ataaaaaagt taccttcatg gtagttcatt aataccacat ccttagttga
     4261 atttatttaa cgtgatacta atacagtttt aattgtataa atattaatat tgtgatgtaa
     4321 tttttatatt ttttaagtac ggagtgagaa atttaaaact ttattggagt attaaatgaa
     4381 ctttccgttt accaccgaac aatttcttga tgttttcaaa gtctataatc ttactgtctg
     4441 gccggcacaa atattcttat taatattagc tctgatggca atctttcttg caatttacag
     4501 aactagatac tctgatgcag ttatagcact aacgctggcg ttttattggt tgtggattgg
     4561 tatagtttat cacttattgt ttttcacaaa aataaatact gcggcttatg gttttgcagc
     4621 attatttgta attcaatcta ttttattttt ttatcttggc ttcattaaac actcattaca
     4681 atttaaatat tcacataata ctaatggaat aatagcaatt gttctcttct tgtatgcatt
     4741 gatattttat ccgcttcttg gatattattt cggacattat tacccggcca caccaacatt
     4801 cggactccct tgtccaacaa caatttttac gttaggtatg ttacttctat tggaaggaaa
     4861 aattagaaag ggattattta ttatccctgt gctctggtct attattggat ttactgctgc
     4921 aattaaatta ggaattcttc aggatatcgg tttacttgtt gctgctgttt taaccgtcgc
     4981 agttttggtg tttggaagaa ctaataaacg agacgcctag atttaaaaat aaaaatgatg
     5041 acttctttaa gaaaagacgt catcataatt aattaaaaca ttatagataa aaagcattat
     5101 tcttttattt tatacttata taaaaatccc tggcgttttt tagcttcttc atcccactta
     5161 acagttgtag tcctcaagaa ttcttgtttg gctgcattta atttttgcat atccagcccg
     5221 atatattttt gtgctttttc tttggtagaa atatctggaa gatcaacagg atatttccac
     5281 ccctgcttta caaatagaat cgcaatttct cttcttgcct gctcaccttt ttgaattgat
     5341 gttcccagaa ctcttaatgc ttcaaccgga gcgtggaaac caagtgcgtt tgctgcagca
     5401 acccaatccc atctccattg agcatgacgg attaacttta atataggttt catctgctct
     5461 tcagttgcac cattatccca tgcaactttt gcttcgatat gagcagcagc taaagttttt
     5521 tctgcaattc gtcttatctc ttctattcta tcctgtctat cgtaaacatc ttgcattaat
     5581 cttgtggttt cttccctgtg gcaaacctga caagtgttgg caatgttatt taaaggtgat
     5641 tgtatatgat gatctgtgaa tttaactcca ccttcagatt tatatggcat atgacaatca
     5701 gaacaggaaa ctccacgaat agcatgaatt cctgttttaa acaattcata atcgggatgc
     5761 tgtgctttta acattggagc tctgcttagt gcatgagtcc aatcagaaaa ttccatagca
     5821 tcgtaataag cctccatgga atcagcacta aatcctctat cccatggaaa tgttaaatac
     5881 ttttcttctt tacctttaaa ataatattca acgtggcatt gtgcgcaaac aagtgatcgc
     5941 atctcctgtc tgctaaagct gctcatatct ttcccctgtc ttttgaatgc ttcaatcaat
     6001 gcaggacgag taattcttaa attcatagtt ttgggatcgt ggcaatcctg acagccaatt
     6061 gggttaacaa tttctgctcc cagatcaatc cattttttct tgtaaaaatt tgatgcgcca
     6121 atttcattca taactcttgg tacgtctgta cttttgcagg tccagcaggt tgcgggcatt
     6181 ggactgtttt cattatcccc ggttcgtaaa atatttctga tatcttcaat tgcatgcccg
     6241 tgccctctgc cctggttata ttcttttgaa aaggcataac ctgcccacat tacaacaagt
     6301 tcaggatatt cctcaagata gtctcgttta accgaaccgc catgcttact tgcataagtc
     6361 gtatccaatg ttttaagata agattcaaac tcacgaggat aattttctcc ccaaacttcg
     6421 ttgcgtggtt cccaatcatt tattggctta accatttgaa gagttacagc ttcacttctt
     6481 cgctctatga tcgatgaagc aaataatcca actaaaaaaa caattaccgc agtagcaaag
     6541 aataaaagcc atcccaacca gggtttgttt tttgttaatt cgcttattgg tttcatttta
     6601 tatacctaaa ttttatttat caattatctt tattcaattc caaacttttt ttaatccatt
     6661 ccgggatagg atcagataaa ccgggaattc tagcatatgg agttgatgat aggctattaa
     6721 ctcttccatg cggaacttca cgatgacaat cccagcaata accatcgcct tcttcctgaa
     6781 tcttttgatt actaattgct ctaagtgaaa tcggatgaat aacatttgaa tgacatctaa
     6841 tgcaattttg ctgcactgca tttttaccag cctgctttat ttgaattacc tgcggttcca
     6901 aacgaaatgt aaacataaat gaatggcgca atccatccga ggctttgaag aaatattttt
     6961 ttataaagtt atcttgtggt acatggcaat cattacaaac tgtaactctg ccatggcttc
     7021 cattctgcca ggttgcatat tgcggcgcca taacatggca gttaacacat gtttccggtt
     7081 tatcggacaa atatgaagtt gcttttgcaa gatgaagaac taaaaataaa agtccaccta
     7141 aaacaccaag actaaatatt atagcgattc gccattgcgg aggaggaata aatgagtgaa
     7201 ttatgttcaa aattttcatt ctgtactctc cccattccct ttaaaaattt taatttattt
     7261 gtatctgtag atatttactg aatttataat cgatcaaact cttttgagca aattaaaaat
     7321 gaacacctga ttttaagctt tttcatttta acgattgaat gatttttacc tcgataagat
     7381 aaaccgtttt agctctaatt tcaataaagt gaaagaatag aattttaagc gcagacctat
     7441 aaaatttaaa ttttatttat tattcaattt tttttgttga ttctgaattt ctacgtcttc
     7501 atttaatttt ttaaactctt ttactccggt tggatgccca taactgttca tatacttata
     7561 atgcatcagg tactcttctt tagtgatttt attctgccag ttacctgtta acatagcaaa
     7621 ccctgttata atcataaata ttgccacaac tccaattgct acaaattttt tgttaattgt
     7681 tttcttaatt ggaaaaagag attttaattc caaagtatct ttaacagggc aagcatcaac
     7741 acaattcagg catgtggaac attcatcaga aattactgtt ttaacactat caacttttat
     7801 aaaagatggg caagctttat tacaaagtcc gcaatcaatg cagctctttg caattctctg
     7861 aatcttgtta gggcttaata aggacattat gccaagtagt gccccgtaag gacaaagaaa
     7921 tctgcaccaa aaacttctga taaaaattga tagcaaaaat aaagcagcaa ttacaatcaa
     7981 tgctgcccgg gaaatatttg caaagaaata atacatctta acatctgaaa caagattgta
     8041 cggactatca agaaaagctt taagtgctaa attggacatt aaaaagacta ccgaataaac
     8101 aagaaaccca agcaataaat actttaaact tcttaaagaa taatccaacc actttggaag
     8161 ttttaacctt cttttaaaaa ttttttctcc aaaatctcca atcaattcag agatatatcc
     8221 aattgggcaa aaccaactgc agaatgattt accgaagact aaagacatca aaacgattga
     8281 aagaaaaata aataatccgg caggatgtgc ggaatgaatt tctccggtag taatgaaaag
     8341 ataaaaactc ataaaagaac ttataggaag gaatccatct acacccggcg gtcgtgaatg
     8401 aaatgcagta gcaccatttg tttccaaata ttgattgaag agataaaatt caattcctat
     8461 ccagacacaa agtgcggcaa ataatgattg aacaataaat cttgtttttt gtataccctt
     8521 ttcatcatta cgaattattt tctttttctt ttttatttca gatagcgttg tcatacactc
     8581 attaaaaact attatacatt atttatttta tttaaagttt tttctttgaa cttatctaaa
     8641 gtttcagagc ttaacatttc ataagctttg gttctcaaag aaccccactg gtcatgaacc
     8701 gggcacggtt tatccggcga gcatttcggg aaaccaagca cgcaggaatt aaaactttcc
     8761 aatccatcaa ttgcatccac tatatctatt aattttattt tagatggatg cttagcgagt
     8821 ttgaaaccgc cagatttacc tttttttgat tcaatgattc cgctttccgt caggctttga
     8881 agaatcttcg aaataaactc ttttggtatt tttaactttc tagagatttc ttcagccggg
     8941 taaactatat tattctcact agcagccata aaaagaactg cctgcaaacc atactcgcac
     9001 ttttttgaga aaaaaactg
//
LOCUS       DASWBY010000916         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_411161, whole genome shotgun sequence.
ACCESSION   DASWBY010000916 DASWBY010000000
VERSION     DASWBY010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411161"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VF870_16520"
     CDS             complement(<1..935)
                     /locus_tag="VF870_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9253854.1"
                     /translation="MKNIIILLALVLTIVSCSQKQNPQKDSEEVTMLKEKIAKFVPVE
                     IQYDESLLTDREKVVLEKLYRASKIIDELFLEQVYIENDQIKSDLISKINNKSILENE
                     PELKPQLELFNIMFGPFDRLEDNKPFIGKNTKPLGANFYPADMSKEEFERWIKDNPKD
                     EKAFTSEFTIIRREDDKLIAIPYSEFFKDKLTAASNLLKEAAEYSDNPSLKKYLNSRA
                     DAFLSNDYYRSDMDWMDVKNNNIEVVIGPYEVYEDELFNYKASFESFVTIKDPVESKK
                     LEVFAKYLNDMEKNLPLDDKHKNFSRGSESPIVVA"
     gene            1106..2770
                     /locus_tag="VF870_16525"
     CDS             1106..2770
                     /locus_tag="VF870_16525"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX9253855.1"
                     /translation="MRYCLYLKPFCQRIAHEFFLFPLLIIILFLQAPSFAEANELDSL
                     SIYSKAPVIIDGHVLFSVRGISSFPAEERANAIRKRIEDVAGDNSIPANSIEIVPGKS
                     SDDLYAQDKFLLSVFDFDSKLEGVRREVVAIGIKKRITEAIYSYRYERKYETLLRKSI
                     YALGATLLTALILLIIFRIMNKVNLVFERRLKVKLDTIETKSFQLVKSNQLWLSLYGL
                     IKGIKFLIVLILVFIYIQYVLGLYPWTRFVSISLFGFFINPVSAIGTALLNFIPDLAF
                     LIVIFFVAKYLLKLVKILFDGLGKGLITISGFDPDWAVPTFKIMRVLLIAFAVIVAYP
                     YIPGSDSAAFKGVSLFLGVLFSLGSSSVVGNLIAGYSMVYRRTFKVGDLVKIDDHLGQ
                     VIESKLFATRLRTPKNEEVIVPNSIVLNTHVINYSTLAKTRGLILHTVVGIGYETPWR
                     QVEGMLKLAAERTSGILKEPPPFVLQKLLGDFAVNYELNVYCNDPINIMKHYTALHQN
                     VLDVFNENNVQIMTPAYEGDPAEPKVVPKDQWFTPVAGKSSETENK"
     gene            2928..>4453
                     /locus_tag="VF870_16530"
     CDS             2928..>4453
                     /locus_tag="VF870_16530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX9253856.1"
                     /translation="MTKRKFILFYTLAALSIAITIFPQSPKRSDVPDILKWNLSDIYP
                     AVSDWQTDIKKVESEIDQFASYKGKLAESSQSLLTALNSYFGMLKTLYKASTYAGNLS
                     NEDVRISENQALLQQISAVGTKLGETASFFEPEILTIPKEKIENFFREKPELNTYSIY
                     IDNIQRLRPHTLSEAEEKILASFGLIAGNQNTVYSIFADGEKPNPKITLSDGKEVELS
                     PATYTGVRSLEIRSDRSKVFETFFNSYGDFKNTLGANLVGKVKKDWVFAKNRNYKSSL
                     ESALNGDNLPPDVYTTLINQVNKSLPTLHRFLDLKKRMLGVDTLHYYDLYTSLVKKVD
                     LKFAVEEGKQVILNALKPMGSDYLSTVKKSFDDRWIDFIPTEGKRSGAYSSGSAFDVH
                     PYILMNWNDDYESLSTLAHELGHTMHSFYSNTNQPFAKSDYSTFVAEIASTTSETLLN
                     NYMVANVKSDEEKLFLLGSYLDLLRTTIFRQTSFAEFELEIHDRIEQGQPLTGDDLCN
                     "
BASE COUNT         1392 a          750 c          769 g         1542 t
ORIGIN      
        1 tttgcaacta caattggcga ctctgagccg cgtgaaaagt ttttatgttt atcatcaagc
       61 ggaagatttt tttccatatc gtttaaatac tttgcaaaca cttcaagttt tttactttca
      121 acaggatctt taattgttac aaaactttca aacgacgctt tgtagttaaa cagttcatct
      181 tcataaactt catacggacc aattacaacc tctatattat tattcttcac atccatccaa
      241 tccatatcgc ttcgataata atcattactt aaaaacgcat ctgcacggga gttcaaatat
      301 ttttttaatg atggattatc agaatactcc gctgcttctt ttaaaagatt tgatgcagct
      361 gttaatttat ctttgaaaaa ttcactatat ggaatagcaa tcaatttatc atcttctctt
      421 cggattatcg taaactcgct tgtaaatgct ttctcatctt ttggattatc ttttatccat
      481 ctttcaaatt cctctttgga catatcagcg ggatagaaat ttgctccaag cggcttggtg
      541 tttttcccga taaatggttt gttatcttca agcctgtcaa aaggaccgaa cattatatta
      601 aatagttcga gttgtggttt aagttcgggc tcattttcaa gaattgattt gttgttaatc
      661 tttgaaatca aatctgattt aatctgatcg ttctcaatgt aaacctgttc aaggaacaac
      721 tcatcaataa tttttgaggc acgataaagc ttttccaata caaccttttc tctgtctgtt
      781 aaaagacttt catcatattg tatttcaacg gggacaaatt tggcaatctt ttcttttagc
      841 attgttacct cctctgaatc tttttgcgga ttttgttttt gtgaacagct tactattgtt
      901 agcacaaggg ctaatagaat tattatgttt ttcattgcat accttgcttt ttaattataa
      961 gaatgtttaa tgtaaattta gtgaagattt taagaaaagt attcctttct atttcttagt
     1021 atttgttttt gattatctgc tttgtttatt tgcggtgtgt ttttgattcg attgttgtaa
     1081 tcaataaaat ttatccggag agaatatgcg ctactgttta tatttaaaac ctttctgcca
     1141 gcgtattgcc cacgaattct ttttgtttcc gttattaata attattctgt ttcttcaagc
     1201 accatcattt gctgaggcaa acgaacttga ttctctttca atttactcaa aagcaccggt
     1261 aataattgat ggacacgttt tgttttcagt acgagggata tcttcatttc ctgccgaaga
     1321 aagagcaaat gcaattcgta aaagaataga agatgtggct ggcgacaatt ctataccggc
     1381 aaactcgatt gaaattgttc caggaaaatc aagtgatgat ctatatgcgc aagataaatt
     1441 tttgctcagt gtttttgatt ttgactcaaa acttgaagga gtaagacgag aagttgtagc
     1501 aataggaata aagaagagaa ttacagaggc aatatattca tacagatatg aaagaaaata
     1561 tgaaactctg ttaagaaaat caatttatgc tttaggggca acacttttaa cggcattaat
     1621 tttacttata atcttccgga ttatgaataa agttaacttg gtttttgaga gaagattaaa
     1681 agtaaagctt gataccattg aaactaaatc atttcagctt gttaaatcta accagttatg
     1741 gctatcgctt tatggtttaa taaaagggat aaagttttta attgttctga tactcgtttt
     1801 tatatacata caatatgttt tgggtttata cccctggacc agatttgttt ctatttcatt
     1861 atttggtttt tttataaatc cagtaagcgc tattggtacg gcgttgctta attttattcc
     1921 tgaccttgca tttttaattg taatcttttt tgttgcaaaa tatttattaa aacttgtaaa
     1981 gatactgttt gatggccttg gcaagggatt aattacaata tccggattcg atcctgattg
     2041 ggcagtgcca acattcaaaa taatgagggt tcttttaatt gcgtttgctg taattgttgc
     2101 ctatccctat atccccggct ccgattcagc ggcatttaaa ggggtttctt tgtttcttgg
     2161 tgttttgttt tcattaggtt cttcttcggt agtcggaaac ctgattgcag ggtactcaat
     2221 ggtctataga agaaccttta aagtcggcga cctggttaaa attgacgatc atctggggca
     2281 agttatagaa tcaaaacttt ttgcaacccg acttcgcact cccaaaaatg aagaggtcat
     2341 tgttccaaac tcaattgtat tgaataccca cgttataaat tattcaaccc tggctaaaac
     2401 acggggattg attcttcata cagtggttgg aattggatat gaaacaccat ggagacaagt
     2461 agaaggaatg ttaaaacttg ctgccgaacg aacttcggga attttaaaag aaccgcctcc
     2521 gtttgtactt caaaaattac tcggggattt tgcagttaac tatgaattaa atgtttattg
     2581 taacgatcca attaatatta tgaaacatta tactgcatta catcaaaatg ttttagatgt
     2641 ctttaatgaa aacaatgtgc agattatgac tcctgcttat gaaggggatc cagcggagcc
     2701 aaaggttgtt cctaaagatc aatggtttac tcccgttgct ggtaaaagta gtgaaacgga
     2761 gaataagtaa ctcggttttt attcttattt ataccggaga aatgataaaa agtttttccg
     2821 gtttaacaat tatttttata taaccactca tcacttagtt tttctaacat tgtgcatttc
     2881 tctgtaagtt tgttttgcaa aaaaaacaaa tacttacagg tggagaaatg accaaacgaa
     2941 aatttattct tttttacaca ctcgctgcgc tttcaattgc aatcaccata tttcctcaat
     3001 ctcctaagag atcggatgtt ccggatattt taaagtggaa cctttctgat atttatccag
     3061 cagtaagcga ttggcagacc gatataaaaa aagttgaatc ggaaatcgat caatttgctt
     3121 cttacaaggg gaagcttgca gaaagctctc aaagtttatt gactgcttta aattcatatt
     3181 ttggaatgtt aaaaactctt tataaagcct caacttatgc gggaaattta agtaatgaag
     3241 atgtacggat ttctgaaaac caggcattgc ttcaacagat ttctgctgtt ggaacaaagc
     3301 ttggtgaaac agcatcattc tttgaacccg aaattctaac cattccaaaa gaaaaaatcg
     3361 aaaatttctt tagagaaaaa cctgagctta atacatattc aatctatatt gataatatcc
     3421 aaagactgcg cccgcatact ttatcagagg cggaagaaaa aatattggca agctttggtt
     3481 tgattgccgg aaatcaaaac acagtttatt caatttttgc cgatggtgaa aaaccaaatc
     3541 caaagattac tctttctgac ggtaaagaag ttgaactaag tccggctact tataccggag
     3601 taagatcact tgaaattcgc agcgacagaa gtaaagtttt tgaaactttc tttaatagtt
     3661 atggtgattt taaaaacact ttgggcgcca atcttgttgg taaagtaaaa aaagattggg
     3721 tttttgctaa gaacagaaat tacaaatcat cactggaatc tgcgcttaat ggcgataatc
     3781 ttcctccgga tgtttacaca actttaataa atcaggttaa caaaagcctt ccaacgttac
     3841 atcgcttttt ggatttaaag aaaagaatgc ttggagtgga tacacttcat tattatgatt
     3901 tatacacatc ccttgttaaa aaagttgatt taaaattcgc tgttgaagaa ggtaaacaag
     3961 taatacttaa tgcactaaaa ccaatggggt ctgattattt atcaacagta aaaaaatcat
     4021 ttgatgacag atggattgat tttattccga ctgaaggaaa acgaagcggt gcttactcga
     4081 gtggctctgc attcgatgta catccttata ttctaatgaa ctggaatgat gattatgaat
     4141 ccttatctac acttgcacat gagcttggac atacaatgca cagtttttat tcaaacacta
     4201 atcaaccgtt tgcaaaatcg gattactcaa catttgttgc tgaaatcgct tctaccacta
     4261 gcgaaacttt actcaataat tatatggttg caaatgtaaa gagcgacgaa gaaaaactat
     4321 ttttacttgg tagctatctt gatctcttgc gtaccacaat tttccgtcaa acttcttttg
     4381 ccgaatttga acttgagatt cacgaccgga ttgaacaggg gcaacctcta acaggggatg
     4441 acctttgtaa tat
//
LOCUS       DASWBY010000917         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_338271, whole genome shotgun sequence.
ACCESSION   DASWBY010000917 DASWBY010000000
VERSION     DASWBY010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338271"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VF870_16535"
     CDS             complement(<1..221)
                     /locus_tag="VF870_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9253857.1"
                     /translation="MFIKSKIYFLLLLAISITIFPQTKRAINVDDLWAMKRIGTYDVS
                     PDGKKIAYTLTSYTFEANKGNTDIYLIDA"
     gene            290..1078
                     /locus_tag="VF870_16540"
     CDS             290..1078
                     /locus_tag="VF870_16540"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9253858.1"
                     /translation="MLRNNKFNLKILIANFVTIILQQNHMQETQNIFLPGGFKQFRVL
                     KSKYVLSDKKVLVIGSNSEKIAEKMIESGAKTVSIIVNDYDSLINSRLNLTKDSVIGI
                     KMMDFDNTDFNAEEFDLIYAQASISLLHRNKIVKEIKRILKSEGTLCVGEITALTKDY
                     PPFVRNIFETSDILPLLNENCSSYYTERKFTILYEEDLSSSLKSFYENTSELLDINID
                     KLTDKEKSYYKKLLNKISHESNAYLKLGADKYIGFKMLILKKNS"
     gene            1216..>2214
                     /locus_tag="VF870_16545"
     CDS             1216..>2214
                     /locus_tag="VF870_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX9253859.1"
                     /translation="MNIKKGDIVEFQIDKYAFEGKGIAKVSKNELLGLTEENGNEKNY
                     VVFVQGSYPGDKVRARLLKIKNSYAEALAINVITPSSERVKAKCKYFGSCGGCKQQDL
                     DYNAQAKYKQQQVEEIFNKLGGFTNFEIESIIPSQNVFHYRNKMEFSFGDKRWLTKEE
                     IAVEGTLDRDFALGLHIPRIFDKVLDVDECFLQSEISNNILNFTRDFFRKKNTSIYST
                     KTHTGFLRNLVIKQASLTNDLMVNLVTSEENDELVGEYSDELRKEIPQVTTVINNINK
                     KFAAVAVGDYEKVIYGSGFIYDEIGRHKFRISANSFFQTNTLQAEKLYQTALDFAEL"
BASE COUNT          808 a          319 c          404 g          683 t
ORIGIN      
        1 tcagcatcaa tcaaataaat atctgtattg cctttattag cttcaaaagt gtagctggtt
       61 aaagtatatg caattttttt tccatccggc gaaacatcat aagttccaat acgcttcatt
      121 gcccaaagat catcgacatt aattgctctt ttggtttgag gaaatattgt gattgagatt
      181 gcgagcaaga gtaagaaata aatttttgat tttataaaca ttgggtctcc gaagtgttca
      241 aattgttaat ccaaaaataa gactgttaat agttaaagac aaagttagaa tgttacgaaa
      301 caataaattc aatcttaaaa tccttatagc taactttgtt acaataattt tacaacaaaa
      361 tcatatgcag gaaactcaaa atatattctt accaggcgga tttaagcagt ttagagtgct
      421 taaatcaaag tatgtgcttt ccgataaaaa agttcttgta attggatcga acagtgaaaa
      481 gattgcagag aagatgattg aatctggagc aaaaactgtt agcattattg taaatgatta
      541 cgattcttta ataaactcaa gattgaatct caccaaggac agcgtaattg ggataaagat
      601 gatggatttt gataacactg attttaatgc tgaagagttc gatcttattt atgcacaggc
      661 ttcaatttct ttgctgcata gaaataaaat tgtaaaagaa ataaagcgca tccttaaatc
      721 cgaaggaact ctttgtgtgg gagaaattac agccttaaca aaagattatc cgccatttgt
      781 aagaaatatt tttgaaactt ctgacatttt gcctctttta aatgaaaact gttcctcata
      841 ttacactgaa agaaaattta cgattttgta tgaagaagat ttgtcttctt ccttaaaaag
      901 cttttatgaa aacacttctg aattattgga cataaacatt gataagctta cggataaaga
      961 aaaaagctat tacaaaaagt tattaaataa gatcagtcac gaatccaatg catacttaaa
     1021 acttggtgcg gataagtata ttggatttaa aatgctgata ttgaagaaaa actcgtaagt
     1081 cattgcgagg atggcacagc cagacgaagc aatctatcga acacttagca gttcatttaa
     1141 gaaggcttac atttattttg cggattgctt cgccgactaa agtcggactc gcaatgacaa
     1201 tcacagaaaa agaaaatgaa tattaaaaaa ggcgacatag tagaatttca aatcgataag
     1261 tatgcatttg aagggaaagg aattgcaaaa gtttctaaga atgaattgct tggattgaca
     1321 gaagaaaacg gcaacgaaaa aaattacgtt gtgtttgttc agggttcata tcccggcgat
     1381 aaagtaagag caagactact aaaaattaaa aattcttatg ctgaagcgtt agccataaat
     1441 gttattactc cttcaagtga aagagtaaaa gcaaagtgta aatactttgg atcttgcggc
     1501 ggatgcaaac agcaggattt agattataat gcacaggcaa aatacaagca gcagcaagtg
     1561 gaagaaattt ttaacaagct tggcggattt acgaattttg aaattgagtc aatcattcca
     1621 tcccaaaatg tttttcatta tagaaacaaa atggagtttt ctttcgggga taagcgctgg
     1681 ttaacaaaag aagaaattgc tgttgaagga acacttgata gagattttgc acttggtctt
     1741 catattccaa gaatatttga taaagtcctg gatgttgatg aatgcttcct gcaatcagag
     1801 ataagtaata atattttgaa ttttacccgg gattttttca ggaagaaaaa tacttctatc
     1861 tattcaacaa aaacccacac aggattttta cgtaatcttg taatcaagca agcttcttta
     1921 acaaatgatt taatggttaa tcttgttact tctgaagaaa atgatgaatt ggttggtgag
     1981 tattctgacg aattgagaaa agagattcca caagtaacaa ctgttataaa taacatcaac
     2041 aaaaagtttg ctgctgttgc ggttggtgat tatgaaaaag ttatttacgg ttcgggtttt
     2101 atatatgatg aaataggaag acataaattc cgcataagtg ctaactcgtt tttccagaca
     2161 aatactttgc aggcagaaaa actttatcaa actgctctgg attttgctga gtta
//
LOCUS       DASWBY010000918         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_636590, whole genome shotgun sequence.
ACCESSION   DASWBY010000918 DASWBY010000000
VERSION     DASWBY010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636590"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="VF870_16550"
     CDS             complement(<1..1680)
                     /locus_tag="VF870_16550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9253860.1"
                     /translation="MSDELIRKYKSKELLELQTASVALADKAFKILWFNKSFKKDVGT
                     GKIKGTSLKSLFNINTPDQKNIADSAKSFVVPLADKNKNIIITPLFSKSKKEVDAYFL
                     EMLPIVDSDYKQSEEKEKLRRNLVFQNELQSILVLLVKEKSVENIAEEILVRCIDVTS
                     SDFGVIVFHHGEDVQNFLYSDNYNLIADKSEVEKSIKYNSSFINKWLEINKRPLLALN
                     HHNNIGYGLTQVLNCESLCISPCYFDNILHAKIFVGKKSGTFSSLDISDIEQFSILLS
                     FTISNIETRALNAALESRLLQAQKLETIGKLSSGMAHDFNNLLSSIFGSIHLLRSRVP
                     DSENVGKLIDNIENCSVRARDLTKGLLSFGKPTAKRKEIVMPNFLLSEIAKVVSQTFP
                     ANIILEEKVDTGLHNIIGNGTEIYQILLNLCVNAKESIEGTGKIVLSASNLLIDDTNI
                     INYPLLDRGKYVKFSVSDTGSGIDEENLSKIFDPYFSTKQKDSGSGLGLYVSYGIIKA
                     HKGHIDVTSNKGAGTTFDVYLPVFEPQKSAKPKESDKIIMLADDEEMLSELL"
BASE COUNT          527 a          301 c          266 g          614 t
ORIGIN      
        1 aagaagctca cttagcatct cttcatcatc ggctaacata attattttat ctgattcctt
       61 aggctttgct gacttttgcg gctcaaaaac ggggagatag acatcaaagg tggtccctgc
      121 gcctttattg cttgtaacat caatgtgtcc tttgtgtgct ttaattatac cgtaagaaac
      181 atataatcct aagccggaac cactatcttt ttgcttagtt gaaaaatatg gatcaaatat
      241 tttggataaa ttttcctcat caattccgct gcctgtatca cttaccgaaa atttaacata
      301 cttgcctcga tctaaaagcg gataattaat aatattagta tcatcaatta gaaggtttga
      361 agcacttaaa actattttcc ctgtgccttc aatcgattct ttagcattaa cgcataagtt
      421 taaaagtatt tgatagattt cagttccatt gccaattatg ttatgaagtc ctgtatcaac
      481 tttttcttca agaatgatgt ttgctggaaa tgtttgcgat acaacttttg caatctcgct
      541 taatagaaaa ttaggcatca cgatttcttt gcgcttagcc gttggtttac caaacgaaag
      601 caaccctttt gtaaggtccc ttgctcttac tgaacagttt tcgatattat caataagttt
      661 acctacattt tctgaatcgg gaacacggct gcggagtaaa tgtatacttc caaaaatact
      721 tgaaagcagg ttgttaaaat catgagccat tccactgctc aatttcccaa tcgtctctaa
      781 tttttgtgct tgaagcaacc ttgattcaag tgcagcattt agtgctcgag tttcgatatt
      841 gctaattgta aatgaaagta gaatagagaa ttgttcaata tcactaatat ccaaagaaga
      901 aaatgttccg gatttttttc caacaaatat tttagcatga agtatgttat caaaatagca
      961 aggggaaata caaagcgatt cacaattaag cacctgcgtt aatccatatc caatattatt
     1021 atgatggttt aatgcaagaa gcggacgttt attaatttcc agccatttgt tgataaatga
     1081 agaattgtat tttatactct tttcaacttc actcttatca gcaattaaat tatagttgtc
     1141 gctgtacaag aaattttgaa catcttcacc atgatggaaa acaataactc caaaatcact
     1201 acttgtaaca tctatgcatc ttacaagaat ttcttctgca atattttcaa ccgatttttc
     1261 ttttactaat aaaacaagaa tgctctgaag ttcattttga aaaacaagat ttcttctgag
     1321 tttttccttc tcttcactct gcttatagtc tgaatccaca atcggaagca tctctaggaa
     1381 atatgcatca acttcttttt ttgattttga aaagagcggg gtgattatga tatttttatt
     1441 tttatctgca agtggtacaa caaaagattt tgcagaatct gcgatatttt tttgatcagg
     1501 tgtatttata ttaaacagag attttagtga ggtcccttta atttttccgg ttccaacatc
     1561 ttttttgaag cttttattga accataaaat tttaaatgct ttatcggcaa gagcaacaga
     1621 ggctgtctgt aattcaagaa gctcttttga tttatatttt ctgattaatt catcagacat
     1681 tcagtagtta atcttgttta agttcgta
//
LOCUS       DASWBY010000919         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_636600, whole genome shotgun sequence.
ACCESSION   DASWBY010000919 DASWBY010000000
VERSION     DASWBY010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636600"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /gene="glgP"
                     /locus_tag="VF870_16555"
     CDS             complement(<1..1520)
                     /gene="glgP"
                     /locus_tag="VF870_16555"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEX9253861.1"
                     /translation="MAKKENETIFFTDKEDPGSFSLSNQFAEHLEFTLVKDKNTATGA
                     DAYTALSLAVRDRMVRSWLRTQKTYHDKDAKRVYYLSLEYLMGRILGNALINLDYYNE
                     CREILDRDGYDLEEIMEKEHDMGLGNGGLGRLAACYLDSMATLQLPAFGYGIRYEYGI
                     FEQDIEHGYQVERADNWLRDGNPWDLLRRSLTYKVQFYGKTETYDKGNGELGFKWVNT
                     ENVYALAYDVPVPGYKNKTVNNLRLWQAKAAIDFEFSDFNKGNYVESVANKNDSETIS
                     KVLYPNDTYVEGKFLRLKQQYFFVSATLQDIIRKYKIGHKKFNKFSEKTTIQLNDTHP
                     VVAIPELMRILIDDENHSWDEAWNITSKTFAYTNHTVVPEALEEWSEQIFGELLPRHL
                     QIIYEINRRFLEEVKANYSKDQNVLDKLSIISGGKDKRVRMANLAIVGSFAVNGVAAL
                     HTNILKKRIFPDFNKIYLKKFVNVTNGITPRRWIVTANPLLTQLISEKIGQEWIKDL"
BASE COUNT          504 a          325 c          252 g          602 t
ORIGIN      
        1 agatctttta tccattcctg tccgattttt tcagaaatca actgagttaa aagcggattg
       61 gctgtcacta tccatcgcct tggagtaatt ccgtttgtaa cgtttacaaa ttttttgaga
      121 taaattttat taaaatcagg aaagattctt ttctttaaaa tatttgtgtg cagtgcggct
      181 acaccattaa cagcaaagct tcctacaata gccagatttg ccattctaac gcgcttatct
      241 ttacccccgg aaataattga aagtttatct aaaacattct gatctttaga ataatttgcc
      301 tttacttctt ctaaaaacct tctgttgatc tcataaatta tttgaagatg tcgcggaagc
      361 aattctccaa aaatttgttc agaccattct tccaaagctt cgggcaccac agtgtgattt
      421 gtatatgcaa atgttttaga agtgatgttc caggcttcat cccagctatg attttcatca
      481 tcaattagta tgcgcattaa ctccggtatt gctacaacgg gatgggtatc atttaattgt
      541 atagttgttt tttctgaaaa tttattaaat tttttgtgcc caatcttata ttttctaata
      601 atatcctgaa gagttgcaga aacgaaaaag tactgctgtt taagtcttaa aaattttcct
      661 tcaacataag tgtcattagg ataaagaact tttgaaattg tttcagaatc atttttgttt
      721 gcaactgatt caacataatt ccctttgtta aaatcagaaa attcaaaatc gattgcagct
      781 tttgcctgcc acaaacgcag gttgttaaca gtcttatttt tatatccggg aaccggaaca
      841 tcatatgcta aggcatagac attctcggta tttacccatt taaaaccaag ttcaccattg
      901 cctttatcgt aagtttcagt ctttccgtaa aattgtactt tgtatgttag tgatcgtctc
      961 agcaaatccc atggatttcc atctcgcagc caattatcag cgcgctcaac ctgataccca
     1021 tgttcaatat cctgttcaaa aattccatac tcgtagcgta ttccatagcc aaatgcagga
     1081 agctgaagtg ttgccataga atcaagataa caggccgcta accttcccaa accgccgtta
     1141 cccagaccca tatcgtgttc tttttccatt atttcttcaa gatcatatcc atcacgatcc
     1201 agaatttcac ggcattcatt atagtaatca agatttatta gtgcgttacc cagtattctt
     1261 cccattagat attctaacga aagatagtaa acacgttttg catctttatc gtgataagtt
     1321 ttttgagttc ttaaccagct tcttaccatt ctatctctta cagcaagcga aagtgctgta
     1381 taagcgtctg caccggttgc agtattttta tccttaacta aagtaaactc taaatgctct
     1441 gcaaattgat ttgaaagtga aaaacttccc ggatcttcct tgtctgtaaa aaagatagtt
     1501 tcattttctt ttttagccat tatatcaaac tccttgaact ttaatctcta taaatgttga
     1561 ggcaaattta gttgcatata attttaatac aaaataataa tgcttttgaa tatttgttgg
     1621 atttagttat gaattttttt gtgcgtgtga ttgaattgat ataataaaca aacccaattc
     1681 ttt
//
LOCUS       DASWBY010000920         4268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_636910, whole genome shotgun sequence.
ACCESSION   DASWBY010000920 DASWBY010000000
VERSION     DASWBY010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636910"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VF870_16560"
     CDS             <1..1068
                     /locus_tag="VF870_16560"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9253862.1"
                     /translation="RKELLARYLRSKNNRIDIFSVDQIWVPRFARWAVPLQKYLDSEE
                     INSIIPNALETCYYNDSLVAVPLYIDIALMFYRDDLLRKFSDYPELKKQINESITWEK
                     FIELGKRFNINSNPFYTFQADDYEGLICSFTELMANQNNPVVDAKGNLRLDTNEGKKS
                     LQLLVDLVNKYNVSPKNVAFLKENESYRFFAQHNGVFLRSWPSMIDDNNEFMSDEIKS
                     NLKMAPVPHFTGTKPGSVYGGWNLMISKFSEKIPEVIKFTKFLLSEESQKIMFEEGRY
                     LPTSSSLYENDLFISKYPELSIFKKLYKTGVHRPFLESYTSVSNVLSFYLNKAIKEEM
                     SVDEALKEATKKINEDAILVK"
     gene            1074..2552
                     /locus_tag="VF870_16565"
     CDS             1074..2552
                     /locus_tag="VF870_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9253863.1"
                     /translation="MNNKKLFEKIKEYHFQFKHVTVLFIILFAFQLIVSFINKAAVKS
                     FLSTTQEWYQKDSAEEIANLTTTSLELVLESIDTKAVISREEENKVIQAFNIIFSQQQ
                     LKHNIQGLYILVRFGDEVYAIDDGRALYTFLLGHSKVEEPTDSKKKEVVEMYKKIESE
                     LSSNEQIKSIITDKKTFNIFVPFVLRGEYIGAIYMQNSPDFSFISNQVISNYDETSVI
                     YLSLILFGLLAMYFISSYTVKERDDTQKMLFEEHETNLKKQINYEKELVFTKRIYHTH
                     HKAEKIMGFIKEDLRTLSAENINDIKYRVSKYSNFISRVIYDMKWFDPPLQTIRNQMF
                     RTNLNEVIKFIVDNIFLRITSKSNAYEIKMETDPKLPLVPINEFVVWEIIEPLIQNSI
                     DHGGEKNIVIKCKTKYDELKKKSYIIIEDNGVGIKKELLNTNEDGIKNLFLENVTSKE
                     TGLQNSGYGCYISYEISKRCGWDIDAENLNEGGCRFTITISN"
     gene            2567..4039
                     /locus_tag="VF870_16570"
     CDS             2567..4039
                     /locus_tag="VF870_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9253864.1"
                     /translation="MIFDNKRINLLLIEDEDYDVRRVMNTVKPFEERIKVLDIVSNGM
                     AALELLQAKKGKFDVVIMDYQIAGGLMGENLISKIKEIDLSVQIIVITKMTINLTDYN
                     FANKLIKAGAFWYCTKYPGDIEEFIYQPTDFILSIFNAYEKSILERERFRSYQKLHKN
                     VEDTLSQKKIVGESPIMIELKEEIKKFAQSNVNTLIRGSSGTGKELVAFNIHYTSNRK
                     YENFVIINCGSLPSQLVESELFGYEKGAFTGADKKKLGLFEIANHGTIFLDEITELPL
                     PAQVKLLRVIQEGEIEKIGRTEKIKVDVRIIAATNRNVEEEVKEKRFREDLYYRLNVL
                     PIFVPDLKKRVTDIPLLIDYFLSSISIDMGKDKPEIPDETMSVFLNYEWPGNVRELKN
                     VVQRLLFSSEVRITPMLAKRALGIQDSASEANEFGFGDIFNIENILPLKDMEKVLRER
                     YFKFVRLNSDSDTDAAKKLGLAPPNYYRMAKELGLKKNEP"
BASE COUNT         1599 a          571 c          767 g         1331 t
ORIGIN      
        1 aggaaagaac tcttagccag atatcttaga agtaaaaata atcggattga tattttttct
       61 gttgatcaga tctgggtccc gcgatttgca cgatgggctg ttcctttgca aaaatatttg
      121 gacagtgagg aaattaattc aatcattccc aatgccttag agacttgtta ctataatgat
      181 tcactggttg cggtacctct ttacatcgac attgcactaa tgttttacag agatgattta
      241 cttagaaaat tttcggatta tccagaattg aaaaaacaga ttaatgaatc aataacctgg
      301 gaaaagttta ttgagctagg taaaagattt aatattaact caaatccatt ttatactttt
      361 caggcggatg attatgaggg attgatttgt tcgtttacag aattaatggc taatcaaaat
      421 aatccggttg tagatgccaa gggtaattta agattagata ctaatgaagg gaaaaaatca
      481 ttacagcttc ttgttgattt ggtaaacaag tacaatgttt caccaaagaa tgttgccttt
      541 ttaaaagaga atgagagcta cagattcttt gcacagcata atggtgtatt tcttagaagc
      601 tggcctagta tgattgatga taataatgaa tttatgagcg atgaaataaa atcaaattta
      661 aagatggctc ctgtccctca ttttacagga acaaaaccgg gctccgtgta tggcggctgg
      721 aatttgatga tttctaaatt ctcagaaaaa attcctgaag taataaagtt tacaaagttt
      781 ttgttgagtg aagaatcaca aaaaattatg tttgaggaag gcagatatct tccaacttct
      841 agtagtcttt atgaaaatga tttattcata tcaaaatatc ccgaacttag catttttaag
      901 aagctatata aaaccggagt tcacagacca tttttagaaa gttatacaag tgtttcaaat
      961 gttttatcat tctatttaaa taaggctatc aaagaggaaa tgtcggttga tgaagcgctt
     1021 aaagaggcaa ctaaaaagat taatgaagac gcgattttag ttaaatgatg tttatgaata
     1081 acaaaaaatt atttgaaaaa ataaaagaat atcattttca gtttaagcac gttacagtgc
     1141 tttttataat tctatttgct tttcaactga ttgtttcatt cattaataaa gcggcagtaa
     1201 aaagtttttt gagcacaact caggaatggt atcaaaaaga ttcagctgaa gaaattgcaa
     1261 atctaactac aacatcttta gagcttgtac ttgagtcgat cgatactaaa gcggtaatca
     1321 gcagggaaga agaaaataaa gttattcagg ctttcaatat tatatttagt cagcagcagc
     1381 ttaaacataa tatacaagga ctttatattc ttgttcggtt tggcgatgaa gtttatgcca
     1441 tagatgatgg gagagcatta tacacatttc ttttaggtca cagtaaagta gaagagccaa
     1501 ctgattcaaa gaaaaaagaa gtcgtggaga tgtacaaaaa aattgagtct gagctaagtt
     1561 ctaatgaaca gataaaaagt ataatcaccg ataaaaaaac atttaatatt tttgttccct
     1621 ttgtcttaag aggcgagtac atcggggcaa tttatatgca gaattctcct gattttagtt
     1681 ttatcagcaa ccaggtaatc agcaactatg atgaaacttc tgttatatat ctttcgctta
     1741 ttttatttgg attgcttgca atgtatttca tttcatctta cacggttaag gaaagagatg
     1801 acacacaaaa aatgctattt gaagaacacg aaactaatct taaaaaacaa atcaattatg
     1861 agaaagaact tgtttttaca aaaaggattt atcacacaca tcataaagct gaaaagataa
     1921 tgggatttat taaggaagac ttaagaactt tatcagcaga aaatattaat gatatcaaat
     1981 atcgggtttc aaagtactct aattttattt cgcgtgtaat ttatgatatg aaatggtttg
     2041 atccgccttt gcaaacaata cgcaatcaga tgttcagaac taatttgaat gaagtaatta
     2101 aatttattgt tgataatata tttttaagaa taacatccaa atcaaatgct tatgaaataa
     2161 aaatggaaac agatcctaaa ttaccacttg ttccaattaa tgaatttgta gtttgggaaa
     2221 taattgaacc gctgattcag aatagtattg atcatggcgg tgaaaagaat attgttataa
     2281 aatgcaaaac aaaatatgat gaactgaaaa aaaaatcata tattattatt gaagataacg
     2341 gtgttggaat aaaaaaagaa ttattaaaca cgaatgaaga tggaattaaa aatctctttc
     2401 ttgaaaatgt aacttcaaaa gaaacaggtt tgcaaaattc aggctatggc tgctacattt
     2461 cttatgaaat atctaaaaga tgcgggtggg atattgatgc tgaaaattta aatgaaggtg
     2521 gatgccggtt tacaataaca atctctaatt aaatggaaaa tgtaaaatga tttttgataa
     2581 taaaagaatt aacctgctgt taattgaaga tgaagattat gatgtaagaa gagtgatgaa
     2641 cacggtcaaa cctttcgagg agcgaataaa agttcttgat atagtctcta acgggatggc
     2701 tgcacttgaa ttgcttcaag ccaaaaaagg caagtttgat gtcgtaatta tggattacca
     2761 gattgccggc ggactaatgg gtgaaaattt aataagtaaa attaaagaaa ttgatctatc
     2821 ggtacagata attgtaataa ccaaaatgac aatcaatctt actgattaca attttgccaa
     2881 taaacttatt aaagcaggtg cattctggta ctgcacaaaa tatcccggtg atattgaaga
     2941 gtttatttat cagccaactg attttatact gagtattttt aatgcttacg aaaaatctat
     3001 acttgaacgc gagcgcttta gatcttatca gaaacttcat aaaaatgttg aagatacatt
     3061 atcacagaaa aaaattgtcg gtgaatcacc aataatgatc gagctgaaag aagaaattaa
     3121 aaagtttgca cagagcaatg ttaacacact aatcagaggt tcatcaggga cgggtaaaga
     3181 actggtcgca tttaatattc attatacaag caatcgtaaa tatgaaaatt ttgtgataat
     3241 aaattgcgga agtttgccga gccagttagt ggaaagtgaa ttatttggat atgaaaaagg
     3301 tgcatttaca ggtgccgata aaaagaaact tggtctattt gaaattgcaa atcacggtac
     3361 aatttttttg gatgagatta ccgaactgcc tttgcctgcg caagtaaaat tgcttcgtgt
     3421 tatacaggaa ggtgaaattg aaaaaatcgg gcgtacagaa aaaataaaag ttgatgtcag
     3481 gattattgcc gcaacaaaca gaaatgttga agaagaagta aaggaaaaga gatttagaga
     3541 agatctttat tacagattga atgtactacc gatctttgtg cctgatctga aaaaacgagt
     3601 tacagatatc ccgctgctga ttgattattt cttatcaagc ataagtatag atatgggcaa
     3661 agataaaccc gaaatacctg atgagacgat gagtgtattc ttaaattatg aatggcctgg
     3721 caacgtaaga gagttaaaaa acgtagtaca aagattacta ttcagctccg aagtaagaat
     3781 aactccaatg cttgcaaaac gtgctcttgg aattcaggac tcagcatctg aagcaaatga
     3841 gtttggtttc ggagatattt ttaacatcga aaatattctt ccgttaaaag atatggaaaa
     3901 agttttacga gaaagatatt ttaaatttgt caggttaaat tctgattctg atactgatgc
     3961 tgctaaaaaa cttggcttag ctcctccaaa ttattatcga atggcgaagg aattaggact
     4021 taaaaagaat gagccctaaa aagaattaat tattattata ataatcataa attattactg
     4081 taataagacc taataaaaag tattcgaatc ctcactaaat ataattgaaa atcaattact
     4141 tttgagatca gcaatggtat attaattgta atttattatg tattataatc cacagaatta
     4201 attaaatctc atagaaatct taaggtgaac tatgaaaaat attacactac tttttaatac
     4261 cattcttt
//
LOCUS       DASWBY010000921         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_637167, whole genome shotgun sequence.
ACCESSION   DASWBY010000921 DASWBY010000000
VERSION     DASWBY010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637167"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..438
                     /locus_tag="VF870_16575"
     CDS             145..438
                     /locus_tag="VF870_16575"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253865.1"
                     /translation="MEAERVLKFFEKERPNDKRPRNAIEAIKAWAEDQRKLGMKDVRK
                     LSLDAHAAARDVKSDDAKFAAHAAGQAVGTWHVPTHALGAFGYAGRAIIANKK"
     gene            813..1787
                     /locus_tag="VF870_16580"
     CDS             813..1787
                     /locus_tag="VF870_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX9253866.1"
                     /translation="MSNWIKILRINPVKKLLSSGDDTLTFYVKRDLLDDFSCKKPHNK
                     EIDRLINKQQSNGSWIFKSSSVDNYPSINHNLVETFKSIRILVGKYEMNKSYEVISKC
                     AEYIFSCQTKEGDIRGILGTQYMPYYCGLFVEYLIRAGYECDGRIESAMKYLILYRQN
                     DGGWVVPLQTVKINKLDDETYRSDPMVADKSLPSSHIATGMTLRAFAVHSSYRKTDIA
                     KKAGELLKSRFFKPDKYNDRKAPEYWIKFQYPYWWTSLISSLDCLYHLGFSIEDKDIK
                     KGIEWLLINQNESGLWATKYDKGDKDKLELSQLWICYSICKVLKLFLK"
BASE COUNT          662 a          304 c          344 g          535 t
ORIGIN      
        1 taaaaagatg gttttctacg atattgaaat aaatatttaa tttaacagta taccgtcatt
       61 aaaatgataa aagcaccaaa acccaaagac agtaaattat ggcgggattc ttattaccat
      121 aaactattcg ggtataaagc agcgatggaa gccgagagag ttttaaaatt ctttgagaaa
      181 gaacgaccaa atgacaagcg tccgcgtaat gctattgaag caatcaaagc atgggcagaa
      241 gaccagcgca aattagggat gaaagatgtt cgtaaacttt ctcttgatgc acacgcagcg
      301 gcccgtgatg taaaatctga tgatgcaaaa tttgctgccc atgctgctgg acaagccgta
      361 ggaacctggc atgttcccac tcatgcttta ggtgcttttg gttatgctgg tagagccatt
      421 attgccaaca agaaataatt gtacgtataa ataaataatc aaacgccgaa ctgctaacac
      481 ggacaataaa gtaaataaac ttgtcacggt ttttgcacat ttttttctac taccgagaca
      541 gttatgtaat tcatgtaaaa ccggaccgcg ccagaaattc taacttttgg tgtaaaccag
      601 gacagttata gaattcgcaa ggaacattgt accgcgacac gcaaaatgct aagaaaaata
      661 ttacccggat ggcaaaaaca cgtgccatgc ttcgctcacg ccggcctcaa aacatcgtat
      721 tttctaagat gattattacc atacagcctc catttgctca gtatcgttta cttgctttat
      781 taccgggtcg ttaggcaaaa tggggaaaca aaatgagtaa ttggattaaa atattaagaa
      841 taaatcctgt caagaagtta ttatcatcag gtgatgacac cttaaccttt tatgtaaaaa
      901 gagacctact tgatgatttt agttgtaaaa aacctcacaa taaggagata gaccgactaa
      961 taaataaaca acaatcaaac ggttcatgga tcttcaaaag tagtagtgta gacaattatc
     1021 cttcaataaa tcataattta gttgaaactt tcaaatcaat tagaatatta gttggcaaat
     1081 atgaaatgaa taagtcatat gaggtaatct caaaatgtgc agagtatatt ttcagttgtc
     1141 aaacaaaaga gggcgatata agaggaattt taggtaccca atatatgcca tattattgtg
     1201 gtttgtttgt tgaatacctg atccgtgctg gatatgaatg tgatggaagg atagaatcgg
     1261 caatgaaata cttaatttta tacagacaaa atgatggagg ttgggttgta cctttacaaa
     1321 cagtgaagat taacaaactt gatgatgaaa cgtatagatc tgatcctatg gttgctgata
     1381 aatctttacc atcttcccat atagcaactg gaatgacatt aagagctttt gcagtacatt
     1441 cctcttacag aaaaactgat attgcgaaaa aggcaggcga attacttaaa agtagatttt
     1501 tcaaaccaga taaatataat gacagaaaag cacctgaata ttggatcaaa ttccaatatc
     1561 cttactggtg gacatcattg atatcttctc tcgattgctt ataccattta ggtttttcta
     1621 ttgaggataa agacattaag aaaggaattg aatggctttt aatcaaccaa aatgaatctg
     1681 gactctgggc tactaaatat gataagggag ataaagataa attggaatta agccaactat
     1741 ggatttgcta tagtatttgt aaagttttga aactattttt aaaataaaca ctttgcctaa
     1801 cacggacaat aaaattaata ggtttgtcac aagttttgct cccga
//
LOCUS       DASWBY010000922         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_637691, whole genome shotgun sequence.
ACCESSION   DASWBY010000922 DASWBY010000000
VERSION     DASWBY010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637691"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1354)
                     /gene="nuoD"
                     /locus_tag="VF870_16585"
     CDS             complement(104..1354)
                     /gene="nuoD"
                     /locus_tag="VF870_16585"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX9253867.1"
                     /translation="MDRLTKDDVHPKILQKLLEDSDITIEDALENEMILNMGPQHPAT
                     HGVLRLLLRLDGETVIGCVPDVGYLHRGYEKMAENMSYYEYIPHTDRLDYISPLANNV
                     AWVLAVEKLAGIEVPVRAQFIRMIVAELARVASHLVAIGSFAMDVGALTVFLWTLRER
                     EKILDIWDILCGARFTNSYTRIGGVANDAQPDALALVKWFVDQLDDNLAECEKLLNTN
                     RIFIERLDGIGVISKEDALSYGYTGPNLRASGVEFDLRRATPYLQYDKVDFKIPTFTE
                     GDSLARYFVRADEIRESAKIVRQVLQLMKPGEIKANLPKSVLPQKAEVYSKMEELIHD
                     FMIVNFGINPPVGEVYHAIEGSKGELGFYLVSKGEGHPWKCKIRSPSFIHIQSLPHLI
                     KGRMISDVIAIIGSIDPIMGEADK"
     gene            complement(1361..1849)
                     /locus_tag="VF870_16590"
     CDS             complement(1361..1849)
                     /locus_tag="VF870_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9253868.1"
                     /translation="MELKELISQKLQANFPEAEFEFSEYRNEFNVKFNKKFVNPICRF
                     LKEDSELEFLLCEDITAVDWAKRKNRFTVVYHIFSLKHNFRLVLKADVDETDCAIDTV
                     SSVWKTSNWQERETYDMYGIKFNNHPDLRRIYMPEEFEYHPLRKDFPLMGIPGSLSLP
                     KK"
     gene            complement(1852..2373)
                     /gene="nuoB"
                     /locus_tag="VF870_16595"
     CDS             complement(1852..2373)
                     /gene="nuoB"
                     /locus_tag="VF870_16595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX9253869.1"
                     /translation="MGLEAKLNSDGFLVTQLDAVVAWARKNSVYPMPMGISCCAIEMM
                     ASADPKYDIARFGSEVMRFTPRQCDLMIVAGTVTYKMSHVVRKIYEQMPDPKWVIAMG
                     ACTSTGGMYRSYSVVQGIDQFLPVDVYVAGCPPRPDNLLNALIQIQNKIGRTRARDFQ
                     NLKLPELNVIQNN"
     gene            complement(2364..2732)
                     /gene="ndhC"
                     /locus_tag="VF870_16600"
     CDS             complement(2364..2732)
                     /gene="ndhC"
                     /locus_tag="VF870_16600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX9253870.1"
                     /translation="MIETYIPIFIVLAIGVVFAVATVMSSVIFGPQRPNREKLSTYES
                     GMKPIGTTHERISIKYYLVAMLFIIFDLEVIFIYPWAIEFKKLYGEIGISVFYSMLVF
                     LIVLELGYLYVYKKGGFKWD"
     gene            2920..4860
                     /locus_tag="VF870_16605"
     CDS             2920..4860
                     /locus_tag="VF870_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253871.1"
                     /translation="MKLNNIKSNNSVLSIYIITAVIILFVSPSSFSQTRVDSLEYLGK
                     FFNVNLFNTLFDKQLNTFHLNSQLQLYGNYNDVLIRINENFGSTFIRNETNNTRDEQH
                     FNLNAKYLFRKDVSFGILGSSSLLSDNRSLGINESAVNYASVFSEVQPIDKLQLNPFG
                     GYSSNTQVGITDNGALYGIEGVLERMNFSDLNISSELRYRNEDILPRRNLIRFIGLSV
                     GNVFDRSVSNNIRTFYSQNRKDFYFEADSITSAEFSIDKNIQSRIETAYQFFDRLSYD
                     GFLDIFSLDLAAGINWRTIDRDNRYKTAELATNIIYDTRIDELKLEIDGATRYNSKSF
                     DGIFRFNYYERDEKHQVKRFDGMTETIYEQKSELEEQKDNNSNRATLSFYGNFKIARS
                     DQFTFSLYQSKLVYDTQSDLNDDDRDELLSIIRLRYSKLLNPYFSAFINAEGTFGHTV
                     YLFASRSSNNNQNRIIRLRTGGDYSGSIVKSFNSFEVSANYTVYDYEDITTNYQSYSF
                     RQFTGIDSTTIKLTIDASLFLYGYIKLSEIGDFNWDNFTARPTRFLQELYLEPRLILN
                     YERTIFSVGLRLFGLNTYFYEQNTRKLSTEYLSLGPIALIDILAWRNLNVVLRGYYEF
                     ITNTDIPDKQQASLLMQVNWKF"
     gene            4908..5318
                     /locus_tag="VF870_16610"
     CDS             4908..5318
                     /locus_tag="VF870_16610"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9253872.1"
                     /translation="MTEPKYYLEIESSPNNLITVEEFVNYFAVELGLDQEKLNGLLLA
                     ITEATTNAIIHGNKNNQLKMVCIYVYVEGKTLTIKIKDEGKGFDPSIVPDPTDPENLL
                     KDSGRGLYLMRVYMDGLTYNQTPEGTEAILQLEL"
BASE COUNT         1809 a          953 c          847 g         1788 t
ORIGIN      
        1 atatatagcg ttgccataac tgcaggtttc ttaacaggat actttgatat ctctttattt
       61 attcgttcaa ggttttcgac agtaaattta aagctgttat tcattactta tccgcctctc
      121 ccataattgg atctatactt ccaattattg ctataacgtc agaaatcatt ctgcctttta
      181 taagatgtgg caaagattga atatgtataa acgagggtga acgtattttg catttccagg
      241 ggtggccttc acctttgcta acaagataaa atcctaattc tcctttggaa ccttcaattg
      301 catggtaaac ttcaccaacc ggaggattga ttccaaaatt tactatcata aaatcatgta
      361 taagttcttc catcttagaa taaacttcgg ctttttgtgg aagaacagat tttggaagat
      421 tagctttaat ttctccgggt ttcattaact gtaatacttg tctaacaatt tttgcgcttt
      481 cgcgtatctc atcggcacgg acgaaataac gtgctaaact atccccttct gtaaatgttg
      541 gaattttaaa atccacttta tcatattgta aatatggcgt tgctcttctt aaatcaaact
      601 caactccact tgcccgaagg tttggacccg tgtatccata acttaaagca tcttctttgg
      661 agataactcc aattccatct aatctttcaa taaaaattct gtttgtgtta agcagttttt
      721 cacattcagc taaattatca tccagttggt caacaaacca ctttactaaa gcaagggcat
      781 caggctgagc atcgtttgca acgccgccga tacgcgtata actatttgta aatcttgcac
      841 cgcaaagtat atcccaaata tcaagaattt tttcgcgctc tcgcaaagtc catagaaata
      901 ctgtaagagc accaacatcc attgcaaaag atcctatcgc aaccaggtgt gaagcgactc
      961 tggcaagttc agcaacaatc attcttataa actgagcacg aacaggaact tcaattcctg
     1021 ctaacttttc aacagctaaa acccaggcaa cattgtttgc taatggtgag atataatcta
     1081 atcgatctgt atgagggatg tactcataat aactcatgtt ctctgccatt ttctcatatc
     1141 cgcgatgaag atatccaaca tcaggaacac agccgattac agtttcaccg tcaagtctta
     1201 gtaacaatct taatacacca tgagttgcag gatgctgagg acccatatta aggatcatct
     1261 cattttccag agcatcttca atagtaatat cagaatcttc taataatttt tgtaagattt
     1321 ttggatgaac atcgtctttt gttaatctat ccataaactt ttatttcttt ggtaatgata
     1381 atgatccggg aatgcccatt agaggaaaat cttttctcag tggatgatac tcaaattctt
     1441 ccggcatata tattcttcgc agatcaggat gattattaaa ttttattccg tacatatcat
     1501 aagtttctct ttcctgccag ttagatgttt tccaaactga agaaacagta tcgattgcac
     1561 aatcagtttc atcaacatct gcttttagga ccaggcgaaa attatgtttt agagaaaaga
     1621 tatgatacac aactgtaaat ctgtttttcc gttttgccca atcaacagca gttatatctt
     1681 cacagagtaa aaattccagt tcggaatctt ctttaagaaa tctgcagatt ggatttacaa
     1741 attttttgtt gaactttaca ttgaattcat ttctatattc agaaaattcg aattcagctt
     1801 caggaaaatt tgcttgtaat ttttgcgaga ttaattcttt caattccata attaattatt
     1861 ctggattaca ttcaattctg gtaattttaa attttgaaaa tctcttgcac gcgttcttcc
     1921 gattttattt tgaatttgaa ttaatgcgtt taaaagatta tcgggacgag gaggacagcc
     1981 ggccacataa acatcaaccg ggagaaactg atcaatcccc tgaacaactg agtatgagcg
     2041 atacattcct cctgttgaag tacatgcacc catagcaatt acccatttag gatcaggcat
     2101 ttgctcataa atttttctaa caacgtgaga cattttataa gtaactgtac ctgccacaat
     2161 cattaaatca cattggcgcg gtgtaaaccg cataacttca gaaccaaatc tggcaatatc
     2221 atattttgga tccgcggaag ccatcatttc aatagcacag caggaaattc ccataggcat
     2281 aggataaaca gaattttttc tagcccatgc aacaactgca tctaattgtg ttactaaaaa
     2341 tccatcagaa tttaattttg cttctaatcc catttaaatc cacctttttt ataaacatat
     2401 aaatatccaa gctcaagcac gattaaaaat accagcatag aatagaaaac agaaattcca
     2461 atttcaccgt acaatttttt gaattcaata gcccatggat aaataaatat cacctcaaga
     2521 tcgaatatta taaaaagcat tgcaacaagg taatatttaa tagaaattct ttcgtgggta
     2581 gtaccaatcg gtttcattcc gctttcataa gtagaaagtt tttcacggtt tggtctttgc
     2641 ggtccaaaga ttacggagga cattacagta gcaaccgcaa atacaacccc gattgctagt
     2701 acaataaaaa taggtatgta ggtttcaatc atcttttttt atgtaaaaat tgttgccaaa
     2761 gttatccaaa aaatgaacga ttgtcaattt aaactcctgc accataattt agatttttca
     2821 accgtttaga aaatatttct gctaaaagtt aagtggttta ttgttgctat aaacattata
     2881 attgcctcag gattttgaat aaaagtgaac gaataatttt tgaaacttaa taatataaaa
     2941 tctaataatt ctgttttatc tatctacatt attactgcag taattatctt atttgttagt
     3001 ccatcctcct tttctcaaac acgagtcgat agtcttgaat atcttggaaa gttttttaat
     3061 gtcaacctgt tcaatacgct tttcgataaa caattaaata catttcatct taattcgcaa
     3121 ttacagcttt atggaaatta taatgatgtt cttatcagga ttaacgaaaa ttttggatcg
     3181 acttttatac gtaacgagac caataacaca cgggatgaac agcattttaa ccttaatgca
     3241 aaatatttat tccgtaaaga tgtttcattc ggaattttag gcagcagctc acttctttca
     3301 gataaccgct ctttaggaat aaatgaatcc gcagtcaatt atgcttctgt ttttagtgag
     3361 gtgcagccga ttgataaatt gcagttaaat ccatttggcg gatactcaag caacacacaa
     3421 gttggaatta ccgacaacgg agctttatac ggtatagaag gtgtccttga aaggatgaat
     3481 ttttcagatt taaatatcag ctccgaactg cgttatagga atgaagatat acttccaaga
     3541 agaaacttaa tcagatttat aggattatca gttggaaatg tttttgatag aagcgtatca
     3601 aataatattc gtacgtttta ctcgcaaaac agaaaagatt tttattttga agctgattca
     3661 attacatctg cagagtttag tattgacaaa aatatccaaa gcagaattga aacagcatat
     3721 cagttttttg atagactttc ctacgatgga tttttagata ttttttctct tgatcttgca
     3781 gcaggaataa attggagaac aattgatcgt gataaccgat ataaaactgc ggagttagcc
     3841 actaatatta tatatgatac cagaatcgat gaacttaaac tcgagattga tggagcaaca
     3901 cgttacaatt caaaatcttt tgatggaata tttagattta attattatga aagagatgaa
     3961 aaacaccagg ttaaaagatt tgatgggatg acagaaacaa tatatgagca aaaatctgaa
     4021 cttgaggagc aaaaagataa taactcaaat agagccacac tttcatttta tggcaatttt
     4081 aaaatagccc gttccgatca atttactttt agcctttacc agagcaaact tgtttatgat
     4141 actcaaagcg acttaaacga tgatgatcgc gatgaattgc tttctataat cagattaaga
     4201 tattcaaaat tgttaaatcc atatttttca gcatttataa atgctgaagg cacgtttggg
     4261 catacagttt atttatttgc aagcagaagc tcaaataata atcaaaacag aattattaga
     4321 ttacgaaccg gcggtgatta ttcgggaagt atagtaaaat cttttaacag ttttgaggtt
     4381 tcggcaaatt atactgttta tgactatgaa gatattacaa ctaattatca aagttattcg
     4441 ttcagacaat ttacaggtat cgattctaca accataaagc ttacaatcga cgcatcatta
     4501 tttttatatg gatatataaa attatctgaa ataggcgatt ttaattggga taattttaca
     4561 gccaggccta caagatttct gcaagaatta tatcttgagc ccagactaat attaaactat
     4621 gaaagaacca ttttttcggt tggtttaagg ttatttggtt taaatactta cttttatgag
     4681 caaaacacaa gaaaactttc tactgaatat ttaagtctcg ggccaattgc tctaatagat
     4741 atactagcct ggagaaatct taatgttgtt cttaggggct actacgaatt tataacaaat
     4801 acagatattc cggataaaca acaagcaagt ttattgatgc aggttaattg gaaattttaa
     4861 ttgaaaccaa tatattcaca ccacttaaat tataacaatt aaacgaattg actgaaccta
     4921 aatattattt agaaattgag agctcaccta ataacttaat tacggtagaa gaatttgtta
     4981 attattttgc tgttgaatta ggcttagacc aggaaaaact caatggatta cttctagcca
     5041 ttacagaagc cacaacaaac gctatcattc atggcaataa gaataatcaa cttaaaatgg
     5101 tgtgtattta tgtttatgtt gagggtaaaa ctcttactat caaaataaaa gatgaaggaa
     5161 agggatttga tccatcaatt gttcctgatc caacagatcc tgaaaatctt ttaaaggatt
     5221 ccgggcgtgg tttgtactta atgcgtgttt atatggatgg tttaacttac aaccaaacac
     5281 ccgaaggtac cgaagcaatt cttcaactgg aattataagt atttaaaagt ccagcaattt
     5341 tctgattttc ttctttatcc cattactctc tttttcaaaa caccatcatt attcttt
//
LOCUS       DASWBY010000923         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_637719, whole genome shotgun sequence.
ACCESSION   DASWBY010000923 DASWBY010000000
VERSION     DASWBY010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637719"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VF870_16615"
     CDS             <1..966
                     /locus_tag="VF870_16615"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9253873.1"
                     /translation="KRLSELLGKEVKLAPDCIGSETKAMVEAMKDGDVLILENVRFHA
                     EEEKNDPAFAKQLAELGDVYINDAFGSAHRAHASTEGVTKYIKICAAGYLMQKELDYL
                     GGAITNPKRPYVAILGGAKISGKIDVINNLLTKVDTLIVGGGMAYTFYKAEGKEIGTS
                     LLEAEKIDLAKEVLANAKKSGIKFLLPKDVVVASEFNNDSHSTVVSVDAMPADKMGLD
                     IGPETIKLFSDELRNAKTIVWNGPMGVFEMENFAKGTNAIAQVLADVTATGSITVIGG
                     GDSAAAITKAGLKDKVSHVSTGGGASLEFLEGKALPGVVALNDVK"
     gene            980..>1614
                     /locus_tag="VF870_16620"
     CDS             980..>1614
                     /locus_tag="VF870_16620"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9253874.1"
                     /translation="MSNYYRPRGYGGGFFSFPPVIKNLLIINGVVFFIQLLMNNLTFD
                     GFSAQLILNKWFALNPINFKDYTSWPFNFQVWQLITYQFMHGGFWHIFFNMFILWMFG
                     ASIEDIMGSRKFLIFYLLAGVSAGLFQLFITPLLGASEAVTIGASGSIFGVMIAYAMF
                     FPDNLIYVYFLIPVKAKYFIGFLVIIEFLAVDSASSGVAHLAHLGGALFGFL"
BASE COUNT          504 a          228 c          346 g          536 t
ORIGIN      
        1 aaaagattaa gcgaattact tggtaaagaa gttaaattag ctccggattg cattgggtca
       61 gaaaccaaag ctatggtcga agcaatgaaa gatggcgatg ttctaattct tgaaaatgtt
      121 cgctttcatg ctgaagaaga aaaaaatgat ccggcttttg cgaaacaatt agcagagctt
      181 ggagatgttt atataaatga tgcgtttgga agtgcacaca gagctcatgc atctacagaa
      241 ggtgtaacaa agtatataaa aatttgtgct gccggatatt tgatgcagaa agaacttgat
      301 tatctgggcg gagcaataac aaatccaaaa cgcccttacg ttgctatttt aggcggcgca
      361 aaaatctcag gtaaaattga tgttataaat aatcttctta ccaaagttga tacactaatt
      421 gtcggtggcg gaatggccta cactttttac aaagcagaag gtaaagaaat tggaacatca
      481 ttacttgaag cagaaaagat tgatcttgca aaagaggttt tggcaaatgc aaaaaaatct
      541 ggaataaaat tcttgcttcc aaaagatgtt gtagttgcta gtgagtttaa taatgattca
      601 cattcaacag tcgtaagtgt tgatgcaatg cccgctgata aaatggggtt ggatattgga
      661 cctgaaacta ttaagttatt ctcagacgaa ttgagaaatg ccaagacaat tgtttggaac
      721 ggaccaatgg gcgtttttga aatggaaaac tttgcaaaag gtacaaatgc tattgcacaa
      781 gttttagctg acgtgactgc aaccggttca attactgtaa ttggcggcgg tgattcagca
      841 gcagcaatta ctaaagcagg attaaaagat aaagtatcac acgtatcaac tggcggcgga
      901 gcttctcttg aatttcttga aggcaaagct ttacctggtg ttgttgctct taatgatgta
      961 aaataaatta aggaatagtt tgagtaatta ttatagacca agaggttatg gaggaggttt
     1021 tttttcattt cctccagtga ttaaaaattt gcttatcata aatggagttg tattttttat
     1081 acaacttttg atgaataatc ttacatttga tggtttttcg gcgcagttga ttttaaacaa
     1141 atggtttgca ttaaatccaa taaattttaa agactatact agttggccat ttaattttca
     1201 agtgtggcaa ctaataactt atcaatttat gcatggcggt ttttggcaca tattttttaa
     1261 tatgtttatt ctatggatgt ttggagcaag cattgaagat attatggggt caaggaagtt
     1321 cttgatattt tatctgttag cgggtgtaag tgccggattg tttcagttgt ttattactcc
     1381 attattaggt gcttcagaag ctgtaacaat tggtgcttca ggttctattt ttggtgtgat
     1441 gatagcttac gcaatgtttt ttccagacaa tttaatatat gtgtactttc taattcctgt
     1501 taaggcaaaa tattttatcg gatttttagt tattatagaa tttcttgctg ttgatagtgc
     1561 ttcaagcggt gttgcgcatt tagcacattt aggcggagca ctattcgggt tcct
//
LOCUS       DASWBY010000924         3225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_413324, whole genome shotgun sequence.
ACCESSION   DASWBY010000924 DASWBY010000000
VERSION     DASWBY010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413324"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="VF870_16625"
     CDS             complement(<1..1778)
                     /locus_tag="VF870_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253875.1"
                     /translation="MENIYHKFHIPVMGTGHSIDSPIRVAHLGIASVMSIVDDILVEK
                     IYRHYAQKFSIDIEEIAKNDPYARSKRITAYLNLVDKIVALKLDEIKQLTFAQGNEKT
                     KYFELLPDESLLKVKYNYFLTIKDQVEKENVAKELTTLMRAGSIDVNIMSKLDRINYA
                     KDGSILSDEFTDAKAALRGYAESTLTSSIVFSAGFNRSLLGYLTQFKDFYRDENGEIK
                     KKIIIKVSDFRSAMIQGKFLATKGLEVSEFRIESGLNCGGHAFASQGYLLPSILREFR
                     DKRSTLAQEFLPLIKAYYEKQNWSFNESNFMCEPLLTVQGGIGTSGEAQRMIKDFGCD
                     STGWGSPFLLVPQATCVDDDTLNLLMNAEKDDLYLSGASPLGVPFNNLRNTGSENWTK
                     NRAESGKPGSACPKGFLISDKQYTDKPICTASRQFQKNKFEEITALQLSNSEKDELLD
                     SMFAKVCLCDHLANGALIKLGISPKTKSPQSICPGPNIVWFNRQYSLQEMTDHIYGRG
                     ESLVSSDRPHMFCQELELYVNYFEKLIGTMGSSEAEIKYLEIFKENLEKGIEYILEIS
                     DETAYPNENLKSIPEFVNAQHQRLKS"
     gene            2198..>3225
                     /locus_tag="VF870_16630"
     CDS             2198..>3225
                     /locus_tag="VF870_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253876.1"
                     /translation="MKKVLCTIIVLIFSASLGYSQVTTLWEKSATAGTKPAWETGSTT
                     RGIGYGLVGANQRLFVVNRNAAFGGKQIFIFNATTGDSVGMLDTTGISGGTLPVNDVE
                     VSSDGKIFVCNLAAGGFFKVYKYDSEVAPPVAVINVDATGRRLGDKITITGTTADNSI
                     IIWAVSADADGEVLKFTTTDNGVSFTSQVVNIGTLASFGSAAVGPLSSGDFYFNAQGL
                     NAKKFSSTGSDLGTVPNTVLTTGGSAIRYLSAILGDEYIVANELSASTNNAKFIKVPG
                     GVLASAAVYGTTPVFGTTSAGGLGDVSVQKVSAYVYNIFVLATNNGFGAYRITIKPSL
                     AGDYYIGAP"
BASE COUNT          995 a          575 c          564 g         1091 t
ORIGIN      
        1 attgatttta atctctgatg ctgcgcattc acaaattccg gaatagattt taggttttca
       61 tttggataag ctgtttcatc ggaaatttcc aggatatatt cgataccttt ttcaagattc
      121 tctttaaata tttccaaata cttaatttca gcttcgctgc tgcccatagt tcctataagt
      181 ttttcaaaat aattaacgta tagttcaagt tcctggcaga acatatgcgg tctgtctgaa
      241 gatactaatg attcacctct tccataaata tgatctgtca tttcctgtaa ggaatattgc
      301 ctgttgaacc aaactatatt tggtcccggg cagattgatt ggggtgattt tgttttggga
      361 gatatcccaa gtttaattag cgcaccattc gcaagatgat cacacagaca aacttttgca
      421 aacattgaat ccaataattc atctttttct gaattagaaa gttgtaaagc tgtgatttcc
      481 tcaaatttat ttttctgaaa ttgacgtgat gcggtacaga ttggtttatc agtgtattgc
      541 ttatctgaaa ttaaaaatcc tttaggacaa gcagaacctg gtttgccgct ttcagcacga
      601 ttttttgtcc aattttctga gccagtattt ctaagattgt taaacggaac tccaagtggt
      661 gatgcaccgc ttaaatacaa atcgtctttt tcagcattca ttaataaatt taatgtatca
      721 tcatcaacac aggttgcttg cggcactaac aaaaacggac ttccccaacc tgttgaatcg
      781 catccgaaat cttttatcat tctttgtgct tcaccgctcg ttccgatgcc accctgaaca
      841 gtaagtaatg gctcgcacat aaaattggat tcattaaaac tccaattttg tttttcataa
      901 tatgctttta ttaacggtaa aaattcctgt gcaagtgttg atcttttatc tctaaactca
      961 cgcaggattg aaggaagcaa atagccttgc gaagcaaaag catgtccgcc gcagtttaat
     1021 cccgattcaa ttctaaactc actaacttca agcccttttg ttgctaaaaa tttcccctga
     1081 atcatcgcag atctaaaatc acttacctta ataataatct tcttctttat ctcaccattt
     1141 tcatcacgat aaaaatcttt aaactgagtt agatatccta atagacttct gttaaatcct
     1201 gcagaaaaaa ctattgatga agtaagagta ctttcagcat atccgcgtaa tgcagctttt
     1261 gcatccgtga actcatcact taaaatacta ccgtcttttg cataatttat cctatcaagc
     1321 ttagacatta tatttacatc tatagagccg gcacgcatta aagttgttaa ctcttttgcc
     1381 acattctctt tttcaacttg atcttttata gtcagaaaat aattatactt tacttttaac
     1441 agactttcat cgggcagcaa ttcaaaatat tttgtttttt catttccttg tgcgaaagtc
     1501 agttgtttta tttcatcaag cttaagggca actattttat caactaagtt cagataagca
     1561 gtaattcgtt ttgatcttgc ataaggatca ttttttgcaa tctcttcaat atcaatagaa
     1621 aacttttggg cataatgtct atagattttt tctacaagga tgtcatccac gatagacatt
     1681 acggaagcta ttccaagatg ggctactcgt atcggggagt caattgaatg ccctgttccc
     1741 ataaccggga tatgaaattt atgatatatg ttttccatta tattattctt cctgtcaaaa
     1801 ctcgaaacaa ataattctaa cctatgtttt ctatgaattt gtaattaaat cacaagctaa
     1861 gctaaataat taatgaagta taaaaagaga gaaaaaaggg gttttacaat acttttacaa
     1921 acaaagaact tggctaggtt tataaattct gatcagttta aatttcagag attaaaatat
     1981 aaaaataatc gttttgaaga ttgtaaaatt gcttctaatt cttggtaatt agtagtcctc
     2041 attcattatt gcgaaagata ataatgcatt tatataatta ttttcataag tttgagtgag
     2101 cataattaat attactattt caaccaatga aaaaaacaat tcaaatttcc gttctatcat
     2161 taaattcaat ttattatcaa ttattagcga ggaacaaatg aagaaagtat tgtgtactat
     2221 aattgttttg attttttctg cgagtttagg atactcgcaa gtaacaacct tgtgggaaaa
     2281 atctgcaacc gctggaacca aacctgcgtg ggaaacgggc agtacaacta gaggaattgg
     2341 ctacggtcta gttggtgcga atcaaagact ttttgtggtt aacagaaatg cggcatttgg
     2401 tggcaaacaa atcttcattt ttaatgctac aaccggagac tctgttggaa tgttggacac
     2461 taccggaatt tccggaggca ctcttccggt taatgatgtt gaagtttctt ccgatggaaa
     2521 gatatttgtt tgtaatcttg ctgcgggtgg attttttaag gtttataaat atgattccga
     2581 agttgcaccg cctgttgctg taataaatgt tgatgcaaca ggtcgaagac tgggagataa
     2641 aattacaatt actggcacaa cagcagataa ttcaattatc atatgggctg tttctgcaga
     2701 tgctgacggc gaagtattaa aatttacaac aacggataat ggagtttcgt tcacatcaca
     2761 agtagtaaat atcggtactt tagcaagctt tggaagtgct gcagttggtc cactgtcaag
     2821 cggagatttc tattttaatg cccaaggatt aaatgcaaaa aagttttctt caaccgggtc
     2881 tgatctgggc acagttccga atacagtgct gactacagga ggaagcgcta tcagatattt
     2941 gtcggctatt ttaggtgatg aatatatagt tgcaaatgaa cttagcgcaa gtacaaacaa
     3001 tgcaaaattt ataaaggtac ctggaggagt tctggcttca gcagcagttt acggtacaac
     3061 ccctgtattt ggtacgactt cagcgggtgg acttggggat gtatctgtcc aaaaagttag
     3121 tgcttatgtt tataacatat ttgtcttggc aacaaacaat ggttttggcg cttatcgtat
     3181 aacaattaaa ccaagtcttg ccggtgatta ttatatcggt gcccc
//
LOCUS       DASWBY010000925         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_637926, whole genome shotgun sequence.
ACCESSION   DASWBY010000925 DASWBY010000000
VERSION     DASWBY010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637926"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VF870_16635"
     CDS             complement(<1..436)
                     /locus_tag="VF870_16635"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX9253877.1"
                     /translation="MQIIHNSSDILAGSVDVYVNDGLAIDDFGFREATPFLELPAGVT
                     LNIGIAAGNSTSVDDTIANFPVVLSADEKYVVFANGLLTDGYTSNPDGRNTDFTLLVK
                     TMARESAAGTGVDLFVLHGSTDAPTVDVKVRELNSATIVDDAA"
     gene            complement(603..1091)
                     /gene="purE"
                     /locus_tag="VF870_16640"
     CDS             complement(603..1091)
                     /gene="purE"
                     /locus_tag="VF870_16640"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX9253878.1"
                     /translation="MSKEIQVGIIMGSDSDLSIMKEASDVLNEFGITNEMKIISAHRS
                     PHLLAEYASNAHERGMKVFIAGAGAAAHLPGVTAAYTPLPVIGVPIKSKSLDGLDSLL
                     SMVQMPSGVPVATVAINGAKNAGILAAQIMGTGNVDLQKKIIDFKKKLSEESIRKNEK
                     MK"
     gene            complement(1115..>1598)
                     /locus_tag="VF870_16645"
     CDS             complement(1115..>1598)
                     /locus_tag="VF870_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253879.1"
                     /translation="QFLDKMKIKKSIYEKKSFYVGDAKIYLLNDSYSYSYKSFSSNDK
                     SGVINIVYGKNSFLFVGDCEHPAEYFLASNFDKMLDSDVLKVGHHGSATGSSEAFLNL
                     VSPDISLVSVGIKNKFNHPAESVIKSLEKINSEIYRTDQLGAVLLKSDGKNIKQVAWK
                     "
BASE COUNT          480 a          319 c          226 g          573 t
ORIGIN      
        1 atgctgcatc atctactatc gttgcactgt ttaattctct tacctttaca tcaactgttg
       61 gtgcatctgt tgatccgtgt aatacaaaca aatctactcc tgtgcctgct gcactctctc
      121 ttgccattgt ctttactaac aatgtgaagt ctgtatttct tccatctgga tttgatgtat
      181 agccatctgt taatagtccg tttgcaaata ctacatattt ctcatctgca cttaatacta
      241 ccggaaagtt cgctattgtg tcatctactg aggtactatt gcctgcggct atacctatat
      301 ttaaggttac tcctgctggc aattcaagga atggagttgc ctctctaaat ccgaaatcat
      361 ctatggctaa tccatcattt acatatacgt ctacactacc cgctagtata tctgatgagt
      421 tatgtattat ttgcactttc gcagtctagc tataaataaa tgactgtgaa gcgaataata
      481 aagcaatgaa aaaatagaat gtttttctca ttttggtctc ctgattagtt attaattatg
      541 aatttaatgt tgtctaatca agtaaacccg aacattttag aaaaagttcc tcacaatgat
      601 aattatttca ttttttcatt tttacgaatt gattcttcag acaacttctt tttaaaatca
      661 attatttttt tctgaagatc tacattgcct gttcccataa tttgtgctgc aagtataccc
      721 gcattttttg caccatttat agctacagta gctaccggaa ctccagaagg catctgaacc
      781 atagataata atgaatccag cccatccaat gattttgact taataggaac acctataacc
      841 ggcagcggag tataggcagc agtaactccc ggtagatgtg ctgcggctcc agcgcctgca
      901 ataaatactt tcattcctct ttcgtgagca ttgcttgcat attctgcaag cagatgagga
      961 ctgcggtgag ctgatattat tttcatttca tttgttattc caaattcatt taaaacatct
     1021 gatgcttctt tcattattga aagatcggaa tcactgccca ttattattcc gacttgaatt
     1081 tcttttgaca taattatcct ttcacatttt aaaattattt ccaagctact tgtttaatat
     1141 ttttcccgtc agattttaat aaaacagcac caagctgatc agttctgtaa atctcagaat
     1201 ttattttctc taatgattta ataacacttt cagcaggatg attaaattta ttttttattc
     1261 ccacactaac caaagatata tccggagata ctaagtttaa aaaagcttca gatgaaccag
     1321 tggcactgcc atgatgcccg acttttaaca catctgaatc aagcatctta tcaaaatttg
     1381 atgctagaaa gtactcggct ggatgttcac aatctccaac aaataaaaat gaatttttac
     1441 cgtacacaat atttataact ccgcttttat cgttacttga gaaactctta taggaataac
     1501 tataagaatc atttaataaa tatatttttg cgtctccaac ataaaaagat tttttttcat
     1561 aaatactctt tttgattttc attttatcaa gaaactgc
//
LOCUS       DASWBY010000926         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ignavibacteriaceae bacterium isolate SMAG_U2694
            k141_638635, whole genome shotgun sequence.
ACCESSION   DASWBY010000926 DASWBY010000000
VERSION     DASWBY010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960345
            Sequence Read Archive: SRR10260012
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteriaceae bacterium (soil metagenome)
  ORGANISM  Ignavibacteriaceae bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria; Ignavibacteriales;
            Ignavibacteriaceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,332
            CDSs (total)                      :: 3,300
            Genes (coding)                    :: 3,293
            CDSs (with protein)               :: 3,293
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Ignavibacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638635"
                     /isolate="SMAG_U2694"
                     /isolation_source="soil"
                     /db_xref="taxon:2651163"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.89667 N 120.74278 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VF870_16650"
     CDS             <1..424
                     /locus_tag="VF870_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9253880.1"
                     /translation="GTVIDSINLVAVTDTAASYGRLPDGSANWEILVPRTRGFSNVVT
                     GVEDNFNSITEYRLNQNYPNPFNPTTTISFKIPATGNVSLKVFNVLGKEVATLINETR
                     SAGNYSIDFNAAGLSSGVYFYQLTTDNFTATKKFTLMK"
     gene            complement(498..2189)
                     /locus_tag="VF870_16655"
     CDS             complement(498..2189)
                     /locus_tag="VF870_16655"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9253881.1"
                     /translation="MKDVLHILYYKTLIFLKVNSPFNFSAVVKSLGSGIVYTLFAYGC
                     FVMTSNTIEYLLVNVKIGSFLLHRFILVVLFIFFIAINVGNMVVSFSTLYKSKEVFHL
                     ITKPISFTKLFLIKFLDNFFYSSTTLLLIITAVLLGYGYYFNLGFWFYPFALFFLILP
                     FMFTAGSAGVIVLLIILKLSGRWGVKKVLSSVGLIYLVSVISFYFISNPIQLVQRVFD
                     YYPNIDQYFGFLESGLVKFLPNYWIAESLYWISQSKFERAIPFIYANLVTSIFVFGIT
                     LFLAKKWYYETWLTSLKVNAELKNTSNKNRGFLGFHKKSYLNGFEESIVKREFLLFFR
                     EPSQWLHLLVMIFLITIFISSISGIDIIILKAYNEYLKTLVYLIVSLFNVFLVASLSL
                     RFVFPLISLEGEGLWKIRSAPINFNDLLLKRLIIYFVIIFFIGQLISFFSNFQFPVML
                     SIVAQINAALTTITLISLNFGMGGIFANYKEKNAIRLASSQGASITFLFTLLYLVLII
                     AILFIPVSNYFYAIQRGINISIWNLLSTSIVLLAMTIIISTVSIKFGLNAFKKDV"
     gene            complement(2189..>2835)
                     /locus_tag="VF870_16660"
     CDS             complement(2189..>2835)
                     /locus_tag="VF870_16660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9253882.1"
                     /translation="TGDFFGFLGQNGAGKTTTIKMISGLYTATSGRILIDGYDIYKEP
                     IKAKSLIGYIPDQPFIYEKLTGREFLFFSGGLYSIEKNKLKNKIDQIIEKLEIGDWVD
                     KRTEEYSQGMKQRITIASALLHDPKLLVVDEPMVGLDPQSALIVKNILKEKANNGAAV
                     FMSTHSLNVAEEICTRIGIIKDGNMIFSDTTEVLEKIKGSDHQNLESLFIHLTK"
BASE COUNT         1031 a          463 c          439 g          902 t
ORIGIN      
        1 aggaacagtt atagacagta ttaatcttgt cgcggttact gatacagcag cctcttatgg
       61 aagattaccg gacggaagtg caaactggga aattttagtt ccaagaacgc gaggtttttc
      121 aaatgttgtt actggtgttg aagataattt taattcaatt actgaatatc gattaaatca
      181 gaactatccg aatccattta atccaacaac tacaattagt tttaaaatac cagcaactgg
      241 taatgtatca ctgaaagttt ttaatgttct tggtaaagaa gttgctactc ttattaatga
      301 aactagaagt gcagggaatt attcaattga ctttaatgct gctggcttaa gcagtggtgt
      361 atatttttat caattaacaa cggacaattt tactgcaaca aagaaattta ctttgatgaa
      421 gtaaaatatt tattaaacag aataatgtta aaagttcatt tcacttttgt gggatgaact
      481 ttttttttgc tttattgtta aacatccttc ttaaaagcat ttaacccgaa cttaattgaa
      541 acagttgaaa tgatgattgt catcgcaaga agcactatgc ttgtgcttag aagattccaa
      601 atggaaatat ttattccgcg ttgtattgca taaaagtaat ttgaaaccgg gataaatagt
      661 attgcaataa tcagaacaag ataaagaagt gtaaatagga aagttattga agctccctgt
      721 gaggaagcta atctaattgc attcttctcc ttataatttg caaatattcc tcccatccca
      781 aagtttaagc ttatcaaagt tattgttgtt agtgctgcat ttatctgcgc aactatagaa
      841 agcataactg ggaattgaaa atttgagaag aaacttatca gctgtccgat aaagaaaatt
      901 attacaaaat aaattataag tcgttttaaa agtaaatcat taaaatttat tggagcactt
      961 ctaattttcc acaaaccttc tccttcaaga ctaatcaagg gaaatacaaa ccttaatgat
     1021 agtgaagcaa ctagaaatac attaaataaa gaaacaatca gataaaccag agtcttcaaa
     1081 tattcattgt aagctttaag aataattata tcaattccgg aaatacttga aataaagatc
     1141 gtgattagaa aaatcattac cagaaggtga agccactgac ttggttctcg gaaaaacagc
     1201 aagaattcac gttttacaat agactcttca aatccattta aataagattt tttgtgaaat
     1261 cccaaaaacc cacgattctt attactcgta ttctttagtt ctgcattaac ctttagtgat
     1321 gttaaccatg tttcataata ccatttcttt gcaagaaaaa gtgtaatccc aaaaacaaag
     1381 attgatgtaa caagatttgc ataaatgaat ggaattgccc gttcaaattt actttgagaa
     1441 atccagtaaa gagattctgc tatccaatag ttcggcaaaa atttaaccaa accgctttcc
     1501 aaaaaaccaa agtactgatc aatatttgga taataatcaa aaacccgttg taccagttgt
     1561 attggattac taataaaata gaatgatatt acactgacta aataaatcaa ccctacagat
     1621 gataaaactt tttttacacc ccatctcccg gataatttca atataatcag cagaacaatt
     1681 actccggcag aacctgccgt aaacatgaaa ggcaatatta gaaagaaaag tgcaaacggg
     1741 tagaaccaga aaccaagatt aaagtaatag ccgtaaccaa gaagaacagc tgttattata
     1801 agcaataaag ttgttgagct gtagaagaaa ttatccaaaa atttaatcaa aaataatttt
     1861 gtgaatgaaa taggttttgt tattagatgg aaaacttctt ttgatttata aagcgtagaa
     1921 aatgaaacta ccatattgcc gacatttata gcaataaaaa aaataaacag cactacaagt
     1981 ataaaccggt gcaatagaaa tgatccgatt tttacattta caagaagata ctctattgta
     2041 ttagatgtca taacaaaaca tccgtacgca aaaagcgtat agacaatacc tgatcccaga
     2101 ctttttacga ctgcggaaaa attaaaaggg gagtttactt ttaagaaaat aagcgtctta
     2161 taataaagta tgtgtaaaac atctttcatt attttgttag atgaataaag agagattcta
     2221 aattctgatg atctgatcct ttgatttttt cgagtacttc agtggtatcg gaaaaaatca
     2281 tgttaccatc ttttattatt cctattcgcg tacaaatttc ttctgcaaca tttaaactgt
     2341 gtgttgacat aaagacagca gcaccattgt tcgctttttc cttaagaata tttttaacaa
     2401 tcaacgcgct ttgaggatct aaaccgacca tcggttcatc aacaactaat aatttgggat
     2461 cgtgaagtaa agctgatgca atagttattc tttgcttcat tccctgtgaa tattcttcag
     2521 ttcgtttatc aacccaatca cctatttcca atttttcaat tatctgatca attttatttt
     2581 taagtttatt tttttcgatc gagtataatc caccactgaa aaaaagaaac tcacgaccag
     2641 taagcttttc atatataaat ggctgatcag gaatgtaccc aattaaagat ttagctttta
     2701 ttggttcttt atagatatca tacccatcga taagtatcct tccggaagtg gcggtgtata
     2761 aaccggaaat cattttgatc gtagtggttt tgcccgcgcc attctgccca aggaatccaa
     2821 aaaaatctcc ggttt
//
